LOCUS       DASWQN010000001         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2375501, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000001 DASWQN010000000
VERSION     DASWQN010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2375501"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VGC82_00005"
     CDS             <1..210
                     /locus_tag="VGC82_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421688.1"
                     /translation="GGWPDQKSGRARPHGLGDAKQIEDREHLLRREIAGLFDQFQHRR
                     PALTLQGLRIRQFSVKHAIRRYRFA"
     gene            925..1320
                     /locus_tag="VGC82_00010"
     CDS             925..1320
                     /locus_tag="VGC82_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0421689.1"
                     /translation="MIKSAVKKMKQRSAGKPDIELGKRIRLRRVEQRISQAELGDQLG
                     VSFQQVQKYEKGVNRVGAARLQQIATALDVPVTFFYDGDGKAREVESLLFLDSAFSLR
                     LLRAYSKIKDQTVQRQLVSLMESIAANED"
BASE COUNT          331 a          483 c          502 g          326 t
ORIGIN      
        1 ggcggttggc ctgatcaaaa atccggccgc gcacggcccc acggcctcgg cgatgccaag
       61 cagatcgagg accgcgaaca cttgctgaga cgcgaaatcg cagggctatt tgaccagttt
      121 cagcaccgga ggccggcgct cactctgcag ggacttcgaa tccggcaatt ttcggtcaag
      181 cacgccattc ggcgctatcg cttcgcatag ggccttcaat tccgaatcgg caatttcatg
      241 cagcttcatg cggagatcga gaattgccac agacaccagc agtgcggtct cgcagttgcc
      301 gtcgtctctt agcgctgcgc ggcattcttc gagcctggcg agaaccgaag gcaatcgttc
      361 gtttgaattc gccaccacct tgttttccgt tggtcgttgg tgaggtaaac ttcatcggac
      421 agcatatccc aagccggccg ataagtcgca cagaaacctg aattcgtgat tacggggatc
      481 gccagacccg gatgaggatg aaagcgccgc gttgcgagcc cgccgtcctc gcggtttgtc
      541 cgcattctgg aaattttagc gtgaaaggat agtccggccc ttggcggccc gggcagcggg
      601 taagcccgtg cccagaggta aatcggccgc accctcccgg aatcggagtt ttccccagct
      661 ctgctcatgt gccgggcacc gaaatcgcac ctctggaggt tgaattacga cagccatatg
      721 atatctggtt gtcgtaccct tcgcggatgc cgggcgcatc cccggggtgc gggatttatt
      781 gagtgtttgc aaatacgtcc gaccgcggaa aaatcccggt ccaacgacga atttgaccca
      841 ttcaagtgag ttcaaggcgg tatataagta tgaatccctg acctatctgt tccggggctt
      901 ttcgaccgaa ggatggtaac cagaatgatc aaatcagcgg taaagaagat gaagcagcgc
      961 agtgcgggca agcccgacat tgagctgggc aagcgaattc ggctgcggcg cgttgagcag
     1021 cggatttcgc aggcggagct tggcgatcaa cttggcgtca gcttccagca ggttcaaaaa
     1081 tacgagaagg gcgttaaccg tgtcggcgcg gcacgtctgc aacagatcgc cacggcgctc
     1141 gatgttcccg tcacgttttt ctatgacggc gacggcaagg cgcgtgaggt cgaaagtctg
     1201 ctgttcctcg acagcgcgtt cagcctgcgg ctgctgcggg cctacagcaa gatcaaggac
     1261 cagaccgtac aacgtcagct ggtgtccttg atggaatcga tcgctgccaa cgaagactga
     1321 cgcagggtcc gctatcgccc ggcggtggcg gaaccatccg gcgttgttcg gttgcgtcgc
     1381 gatcgtggac tagtgagggg ccgggcgaag ccggcttcca cttttctgac cgcgcggccc
     1441 gcgatcgagg cggcttgacc gcacgcccgc ctgctgtgcc tgcggcgtcc cgacagggca
     1501 tcgaacggca atccaccgac gaggtatcgc cgctggcccg tcggccgcgg ccataccagc
     1561 tgttcgccac agaaagttgc gtttccggac gacatcgatg cgtccccgcg cagcggtggg
     1621 gaaagccccg ctggctgccg cg
//
LOCUS       DASWQN010000002         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_132419, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000002 DASWQN010000000
VERSION     DASWQN010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132419"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VGC82_00015"
     CDS             <1..893
                     /locus_tag="VGC82_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY0421690.1"
                     /translation="NQVVRAVDDVSLSIAHGEVLGLVGESGCGKSTLGRMVANIAPPT
                     RGEVLFNGRDIAALPPNEAKAASLKVQMIFQDPYASLNPRLRVADIVGEAPLVHGLVS
                     RANYDNYLDAQLRQAGLDPDYKRRYPHQFSGGQRQRIGIARALAVQPEFLVCDEAVAA
                     LDVSIQAQILNLFMDLRARLNLTYLFISHDLGVVEHLSDRVVIMYLGRVVEEAATEEI
                     FAHANHPYTQALLAEVPRIESRKRRFTAVSGEIPSPLDPPSGCHFHPRCPHAMPRCSV
                     EIPELREIAAGHRSACHLNG"
     gene            1011..1493
                     /locus_tag="VGC82_00020"
     CDS             1011..1493
                     /locus_tag="VGC82_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0421691.1"
                     /translation="MTIKVGDTIPSMKLMVATADGPKETSTDEIFKGKKVVLFAVPGA
                     FTPTCSVKHLPGFVQNADAMKAKGVDTIACIAVNDVFVMGAWGKEQGTGGKITMLADG
                     AAAFAKAIGLELDLNARGMGWRSQRYALVAEDGKVTHLGVEAPGGFELSTADAVLAAL
                     "
     gene            complement(1615..>1781)
                     /locus_tag="VGC82_00025"
     CDS             complement(1615..>1781)
                     /locus_tag="VGC82_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421692.1"
                     /translation="PDKVQEYRSGKDKLFGFFVGQTMKAMAGKGNPGLINETLKRRLG
                     AAAAHPRPGS"
BASE COUNT          310 a          558 c          588 g          325 t
ORIGIN      
        1 cgaaccaggt ggtcagggca gtcgatgacg tcagcctgag catcgcgcat ggcgaggttc
       61 tgggactcgt gggcgaatcc ggctgcggca agtcgacgct tggccgcatg gtggccaaca
      121 tcgcgccgcc aacaaggggc gaagtgctgt tcaatggccg ggacatcgcg gccctgccgc
      181 cgaacgaggc gaaggcggcg tccctgaagg tgcagatgat cttccaggac ccctatgcct
      241 cgctgaatcc aaggctgcgg gtggccgata tcgtgggcga ggcgccctta gtccacggcc
      301 tggtatcccg ggcgaactac gacaactacc tggatgcgca actgcgccag gcgggcctcg
      361 atccagacta caagcgtcgc tatccgcatc agttttccgg cggccagcgc caacgcatcg
      421 gcatcgcccg agcgttggcg gtgcaaccgg agttcctggt gtgcgatgag gcagtggccg
      481 cgttggatgt ctcgatccag gcgcaaatcc tgaacctgtt catggacctg cgggcgcgcc
      541 tgaacctgac ctacctgttc atcagccacg atcttggtgt ggtggagcat ctgtcggatc
      601 gggtcgtgat catgtacctc gggcgggtgg tcgaagaggc agcgacagaa gagatcttcg
      661 cccatgcgaa ccacccctat acgcaggcgc tgctcgccga ggtgccgcgc atcgagtcgc
      721 gcaaacgccg gttcaccgcg gtcagcggag agatccccag cccactcgat ccgccttcag
      781 gctgccattt tcacccccgc tgcccgcacg cgatgccgcg ttgttcggtg gaaatccccg
      841 aattgcggga gatcgccgcg ggacaccgca gtgcctgcca cctgaacgga tgaagcggcc
      901 gggcgtggga ggcgtggggg tgacttccgc caccggcccg gctaggaact ggattctgtc
      961 gcgcttttgg tgttcgcggc atctcacgaa ccctgcggga gcaattaatc atgacgatca
     1021 aggtcggcga tacgattccg tccatgaaac tgatggtcgc gacggcggat ggtccgaaag
     1081 agacctccac cgacgagatc ttcaagggca agaaagtggt cctgttcgcg gtccccggtg
     1141 cctttacccc cacctgcagc gtcaagcacc tgcccggctt cgtgcagaac gcggacgcga
     1201 tgaaggccaa gggcgtcgac accattgcct gtatcgccgt caacgacgtt ttcgtcatgg
     1261 gtgcctgggg caaagaacag ggcaccggcg gaaagatcac catgctcgct gatggtgctg
     1321 ccgcctttgc caaggcgatc ggcctggaac tggacctgaa cgcccgcggc atgggctggc
     1381 gcagccagcg ttacgctttg gtcgccgagg acggcaaggt cacccatctg ggggtcgaag
     1441 cacccggcgg attcgaattg agcacggcgg acgcggttct ggcggcgctt taggtcccga
     1501 cccggtggcc cgcgtggccg ccggtgaagc ttatggtccg ggcccggcgg cccccgtggc
     1561 ccgcgttgat gcttatgatc ccggcccggt ggcctgcgtg gccgccggtg aagcttatga
     1621 tccgggccgt ggatgcgccg ccgcggcgcc gaggcggcgt ttcagggttt cgttgatcag
     1681 accaggattg cccttgccgg ccatcgcttt catcgtctga ccaacgaaga agccgaacag
     1741 cttgtccttg cctgaacggt attcctgcac cttgtccggg t
//
LOCUS       DASWQN010000003         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_264589, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000003 DASWQN010000000
VERSION     DASWQN010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264589"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1130
                     /locus_tag="VGC82_00030"
     CDS             336..1130
                     /locus_tag="VGC82_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY0421693.1"
                     /translation="MPLTTNTTKRRLAAGQMGLGFGVHHLRTVAAPVLAAATGHDWMF
                     IDAEHGAFNIQEITQLCIAALPTGVTPIVRVCAGALDEATRALDNGALGIVVPHVDTA
                     AEARQIAGAFHYPPMGHRSWGGPPAVYGYQPPSTAEAQAAINGEILTIVMLESPEAVE
                     NADAIAAVAGVDVLFIGTSDLTAELGISGQMGHPKVIDAYHAVGEACRKHGKTLGMGG
                     VYDEENASRYVRMGARFLLTGSDHSYLMSGANQRSSFFNALPGAPG"
     gene            1177..>1794
                     /locus_tag="VGC82_00035"
     CDS             1177..>1794
                     /locus_tag="VGC82_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0421694.1"
                     /translation="MATFKSLLDGFEAATTPITAAALGVAPPLQFVGPAALAAPTGGY
                     SAIYSFGDSLSDAGNVSLATLGNLPVAGIYSGGRFTNGNVWVQDLSQNLGLPAVKPSL
                     AGGTDYAYGGAETGTTAVHANNPTDLPSQLGQFAASVPNPSPTALFTVWAGANDVLDI
                     ANSTETPVQQQASVHQAVVNETAFVAGLVAHGAKDLVVMGVPDLAK"
BASE COUNT          338 a          581 c          547 g          328 t
ORIGIN      
        1 aggtttggag cttcctttcg ccgttttgga agccgggcag gcgcagaatc gtgattcggg
       61 cttcgcgtaa agccaaggcc taaaatttct gcgacgaccc tcgcatcata gttcgtgagc
      121 attttgtccg cttttgatca attgggattg agtagcgccc catgtggtcg gcagcaaaga
      181 ggaaatcgag atggcggcgt gccctgatgg agcagtgctg ccgtcagcca gtatcctttc
      241 tccctcgggg tcgtaagccg accgaccggt aacgccgcgg attgaccgcc gcccgcgccc
      301 atgctgctaa caggcaaaca aggaaagtgg aaacgatgcc cttgacaacc aacacgacaa
      361 agcggcgcct cgcggctggc cagatgggcc tgggtttcgg cgtccaccac ctgcgcaccg
      421 tcgccgcccc ggtcctggcc gcggcaacgg gccacgactg gatgttcatc gacgccgaac
      481 acggcgcctt caacatccag gagatcaccc agctctgcat cgccgcccta ccaaccggcg
      541 tgacgcccat tgtgcgcgtt tgcgccggag ccctcgatga ggcgacaagg gcgctggaca
      601 acggcgcgct tggtatcgtc gtgccgcatg tcgataccgc cgccgaagca aggcagattg
      661 ccggcgcctt ccactatccg ccgatgggcc atcgaagctg gggtggccca ccggccgtct
      721 atggctacca gccgccatcc acggccgagg cgcaggccgc gatcaatggc gaaattctca
      781 ccatcgtgat gctggaaagc cccgaggccg ttgaaaacgc cgatgccata gccgccgtcg
      841 ccggcgtgga cgtgctgttc atcggtacct ccgacctgac ggccgaactg ggaatttcgg
      901 gccagatggg acatcccaag gtgatcgacg cctaccacgc ggtcggcgag gcctgtcgca
      961 aacacggtaa gacgctcggc atgggcggcg tctatgatga ggaaaacgcg tcgcgctatg
     1021 tccgcatggg cgcacgcttc ctgctgaccg ggtcggacca cagctacctc atgagcggcg
     1081 cgaaccagcg ttcatcgttc ttcaacgctc tgccaggcgc ccccggttaa accgtagtta
     1141 atgcaactgt gccgtagcat ttggcgttga ggcggcatgg caaccttcaa atcccttctc
     1201 gacggcttcg aagccgccac cactccgatc accgccgccg cgctcggtgt agcaccgccg
     1261 cttcagttcg tcgggcccgc cgctttggcc gcgccgaccg gcggatacag tgcaatctat
     1321 tccttcggcg acagtctgtc cgatgccgga aatgtctccc tcgccacgct tggcaacctg
     1381 ccggttgctg gcatttacag cggcggacgg ttcaccaacg gcaatgtctg ggtgcaggat
     1441 ctgtcacaga atctgggcct gccggcggtg aaacccagcc tggccggcgg aaccgactat
     1501 gcctatggcg gagcagagac cgggaccacc gcggttcacg ccaacaaccc gacagacctg
     1561 ccgtcgcagc tcggccagtt tgctgcttcc gtgccaaatc cttcgccgac cgccttgttt
     1621 acggtttggg cgggagcgaa cgatgtgctg gatatcgcga actcgaccga gacgccggtg
     1681 cagcagcagg ccagcgttca ccaggcggtc gtgaacgaga cggcttttgt cgctggcctg
     1741 gtggcacatg gtgcaaaaga cctggtcgtg atgggcgtcc ccgatctggc aaaa
//
LOCUS       DASWQN010000004         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1980282, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000004 DASWQN010000000
VERSION     DASWQN010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980282"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VGC82_00040"
     CDS             <1..1324
                     /locus_tag="VGC82_00040"
                     /inference="COORDINATES: protein motif:HMM:NF033540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEY0421695.1"
                     /translation="LISRYESFFRTRTRTCAAIAARYLRGLAQAEVCQLAAMAAVVEG
                     GCAQQFQHFISNSPWDHKPVVAQIGRDADQLLGDKPDSCLILDECSFPKQGDRSVGVA
                     RQWSGRLGKVDNCQVAVFGVLTDGARHAPVDMRLYLPKVWIDDPARCDGAEVPQTARK
                     LTSKSEHALDIVRQARARGVRFGWVGVDAGYGKEPAFLRALDTMQEVFVADVHRTQRV
                     WPEEPELRVPPSRPGRGRPASQRQAATDPVSVEALVKTFAAADWTRCVLRDSTRGELR
                     VDIASRRIWLWDGEEATPQCWHLIVRREVKSSETIKYTLSNAPADTPVRRLGQMQGHR
                     YWIERTFEDAKGQCGLGDYQVLGWRAWHHHVTMVMLAMLFIAEQRAAHQPGLALLTPR
                     DIVEMLKESLPCKPEGRDALIARINQRHARRRGAIESRYRTQQKTVPP"
BASE COUNT          360 a          575 c          622 g          328 t
ORIGIN      
        1 cctgatcagc cggtacgaga gcttctttcg cacccgcacg cgcacctgtg cggcgattgc
       61 cgcccgctac ctgcgcggac tggcgcaagc cgaggtctgc cagttggccg cgatggccgc
      121 cgtggttgag ggcggctgcg cgcagcagtt ccaacatttc atcagcaact ctccgtggga
      181 ccacaagccg gtcgttgcac agatcggccg ggacgccgat cagctgcttg gcgacaaacc
      241 ggatagttgc ctgatcctcg atgagtgcag ctttccgaaa cagggagacc gttcggtcgg
      301 ggtggctcgg caatggtcgg ggcgtctggg gaaggtggac aactgtcagg tggcggtgtt
      361 cggcgtgtta acggacggtg cgcgacatgc gccggtcgac atgcgacttt atttgccgaa
      421 ggtctggatc gatgatccgg cacggtgcga tggggccgag gtcccgcaga ccgcgcgtaa
      481 actgacctcg aaaagcgagc acgcgctgga catcgtgcgt caggcgcgtg cccgcggcgt
      541 gcggttcgga tgggtgggtg tcgatgccgg ctacgggaag gaaccggctt tcttgcgcgc
      601 cctggacacc atgcaggagg tgttcgtcgc ggacgttcat cgcacccagc gggtttggcc
      661 ggaagaacct gaactgcggg tgccgccgtc caggccaggc cgtgggcgtc cggcatcgca
      721 gcgacaggca gcgactgacc ccgtcagcgt cgaagctctg gtcaaaacat ttgccgcggc
      781 cgactggacg cgctgcgtcc tgcgcgacag cacgcgtggt gagttacgtg tcgacatcgc
      841 aagccggcgc atttggctgt gggatggcga ggaagccacg ccgcagtgct ggcatctgat
      901 cgtccgccgc gaggtgaaat cgtcagagac gatcaaatac actctgtcca atgctccggc
      961 tgatacgccg gtgcggcgtc tgggccagat gcaggggcac cgctattgga ttgagcggac
     1021 cttcgaagat gccaaaggcc aatgcggcct gggagactac caggtgctgg ggtggcgcgc
     1081 ctggcaccac cacgtgacga tggtgatgct tgcgatgcta tttatcgccg agcagcgcgc
     1141 ggcgcatcaa ccgggcctgg cactgctgac accgcgcgat atcgtcgaaa tgctgaagga
     1201 gagtttgccg tgcaagccgg agggccggga tgccctgatt gcccgcatca accagcgcca
     1261 cgcacgcaga cgcggcgcca ttgagtcccg ctaccggacc cagcagaaga ccgttccgcc
     1321 ttgagccaaa tccacgcctg gcaggctgcc gggcccgggc aaaccgggca cgcgaagcgg
     1381 ccgagttagc caccctccct ataacgatcg tgatgctggc aatgctgttc atcgccgggc
     1441 aagcccccgc gtatgaccag ggcctggaat tggcgatgcc acaatcaatt gccacgctgc
     1501 ggagcgaggc tacccggcaa atggccaggc gtatcgaacc gaagcgatac cctggtcgcc
     1561 tcgatgggtt ggcgaaacgc gtccagccat ggggcattgt acctcattgc cgttgtcagc
     1621 agacaacgga ccaaagcacg agcacaaatc gtgccaagta atgcgactct gtcaaactaa
     1681 gggcctgttt gggaaagaac gctccggttc gggctcggtc gcccgatacc gtcatggccg
     1741 gcgcaggccg gccacccacg actttgttga gacaaacact gcaagtcgtg gatggcgggc
     1801 cttcgcccgc catgacgatg aaaattgtct gcctcgctcc cgccgattcg gatatttcct
     1861 gacaagtcct cagaggcacg cacag
//
LOCUS       DASWQN010000005         4722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_924872, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000005 DASWQN010000000
VERSION     DASWQN010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924872"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..838)
                     /locus_tag="VGC82_00045"
     CDS             complement(11..838)
                     /locus_tag="VGC82_00045"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0421696.1"
                     /translation="MRRFLPGRLRTLLSGESAEPQKAVAKFELRPPAPQNAVDIFAGK
                     WASDLTPVCDVTGTGSAALFRDVRPGQAADSLGHDGRLDGFRLLELGPLEAAHSYQLE
                     QLGASEIIAVEANTEAFLKCLVVKEILGLKNVRFLCGDIVAYLKSCTRRFDMIFCSGV
                     LYHMSDPLELIRLMSLHTDKCFVWTHYFSNEASNDKMERTARTVSRFGFSATYHELEY
                     PDPGFDRFWGGNRPVQAWMEKNEIFAAFAHFGFAHCKLLDEDPDHPAGRCMTFAAYR"
     gene            1140..1994
                     /locus_tag="VGC82_00050"
     CDS             1140..1994
                     /locus_tag="VGC82_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY0421697.1"
                     /translation="MDRQFPRLAVLPTMVLMLLVFGVPLLFSAWLSLEAWSPDQTLFG
                     GKFAGLANYQDLLTDPEFTSTLTLTLGYTAAVVAAELGVGLGIALLLNIDLPWIGLFR
                     TLLIVPMMITPVVAAFCWKLLLDPDHGVINFFLQQHIVWLGRPDTALLSVSVVNIWQN
                     APYVSILLLAGLRSLPHEPMEAARIDGASRLQMFWHITLPLLRPYLLVALLLRMIFEF
                     RSFDNVYVMTSGGPANATMMLSMYTYLASFVRFDLSLGAAASWMMLLISLIMCGCFIA
                     MVRRRDAN"
     gene            1994..2872
                     /locus_tag="VGC82_00055"
     CDS             1994..2872
                     /locus_tag="VGC82_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0421698.1"
                     /translation="MAAAGRRQMAAAGRRRAAGRKRMEALAVYVALSVLTVAFLFPLL
                     WIGGLSLKTRLQVFASPPLFLWWPTFENYAATLSRADFGQAFVNSLLISAGAVSLSLL
                     VGVPAAYALARFSFAGRNFLFFALLSMRMLPPIAVLVPMYVLFSAFGLTTTRTSVVLA
                     YTTFSLPLVVWIMRGFFEDLPRELEESAWVDGASRLQAFVHVVLPLIKPGLVAACILC
                     LQLAWNDFLFAAVLTNNATRTLPVLMAAFSGGDTGVDWGGMTASGVLVILPVILFSFA
                     AQRHLVAGLSSGAVKG"
     gene            2878..4170
                     /locus_tag="VGC82_00060"
     CDS             2878..4170
                     /locus_tag="VGC82_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0421699.1"
                     /translation="MTGLNRRQLLAASAASTLPLPALGQGKPDKLVYVGDNGPWHWAL
                     VEEVAPAFEKATGIKIDFTLLPVDPWTARLKAELSSGSSGIDIVQWSVGMAGWISPHM
                     LDHEVVASEIMAKDPSWNWDDFLSGSKRAATYDGKMAGIPYRVTTGVMHYQKALLEQA
                     GITKLPETFAELEKAAIAANTPPQRYGFGISGRQGPAILSSFLPWLYSCGGNLLDFKT
                     GEILINNEKGVAALDFWTGLILKDKVVPPEAMTWEYDEIVAGGQNDRYAMVQTFAPYG
                     TLINDPKISKTGGKWTWATVPGQTAKDQGRTWVDGHFMGIPKYTRNKDWSLAFIQMAC
                     SKDWMRRSMVRGNAPPRGSVLRDPEMVETIGWPPTAATAIETGFPTPAQPVWSTLQLS
                     LRTGLSQAVLGQKTAKQALDDVAVDWQRGLRRAGVGKG"
     gene            4167..>4722
                     /locus_tag="VGC82_00065"
     CDS             4167..>4722
                     /locus_tag="VGC82_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0421700.1"
                     /translation="MKRRSLLTAGVALALPSVARAGAKPEKLVFVGDNGPWHWCLVEE
                     VAPAFEKATGIKIDFTLLPIDALTARLKGELNSGSPNIDIIQWTAPFAGWLAPHMADH
                     SKLLADNAGRHPDFDWDDFLPSVRDMASYQGQLLGIPYRVTTSILNYQKQLLSDAGFA
                     TPPDNWDSFLAAAQATTKPPERYGL"
BASE COUNT          857 a         1471 c         1464 g          930 t
ORIGIN      
        1 aaccccgcca tcaacgatag gcggcgaacg tcatacatcg accggcagga tggtccgggt
       61 cttcatcgag aagcttgcag tgcgcgaacc cgaaatgcgc aaacgcggcg aagatttcat
      121 ttttttccat ccatgcctga accgggcggt tgccgcccca aaagcggtca aacccgggat
      181 ccggatactc cagttcatgg taggtcgcgc tgaacccgaa ccgagacacg gtgcgcgccg
      241 tccgctccat cttgtcgttg ctggcttcgt tcgaaaaata atgcgtccag acaaagcact
      301 tgtcggtatg cagggacatg agcctgatca gttccagcgg atcggacata tggtaaagga
      361 cccccgagca gaagatcatg tcgaaacgcc gggtacagga ttttagataa gcgacaatgt
      421 cgccgcaaag aaaccgcaca ttcttcagcc ccaggatttc cttaacgacc aagcatttaa
      481 gaaatgcctc cgtgttggct tcgacggcga tgatctcgga cgcaccgagc tgctccagtt
      541 gatagctgtg ggcggcttca agcggaccca attcaagcag acgaaagccg tccagccggc
      601 catcatgacc caacgaatcc gctgcctgcc ccggccggac atcgcgaaaa agcgcggcac
      661 tcccggttcc ggtcacgtcg caaaccgggg tgaggtcgct ggcccacttc ccggcgaaga
      721 tgtccaccgc gttttgcggg gcgggaggac gaagttcgaa cttggccacg gctttttgcg
      781 gttcggccga ttctccactc aagagcgtcc gcagccggcc cggaagaaat cgtctcatgt
      841 catcattccc aattgctcgc tcgggcttcc aagcgaatat ctcctaccgc agggtcattg
      901 tcacgacgat ggcccccctg gggtcttcca aatcctggat gcctcctgaa ggcatcgcgg
      961 caagccggcg cgcgaggtgg cgttcccgtt cggcctgatc taacgtcctg ctgaacgtct
     1021 gcctgcgaac ggagcggccg ggaggaacga caaagacgat gggaggaacc acacaggtcg
     1081 ctgctggcgg cagcctcgcc caccttacta ggcgccgggc caggaaacgt acgagctggg
     1141 tggatcgcca gtttccccgt ctcgcggtgc tgccgacgat ggtgctgatg ctgctggtgt
     1201 ttggcgtccc gctgctgttt tccgcttggc tgtcgctgga ggcatggtcc ccggaccaaa
     1261 ccctgttcgg cggcaaattc gccggcctcg caaactacca ggacctgctg acggacccgg
     1321 agttcaccag caccctgacc ctgaccttgg gatataccgc ggccgtagtg gcggctgaac
     1381 tcggcgtcgg actgggcatc gccctgctgc tgaacatcga cctgccgtgg atcggcctgt
     1441 tccgcaccct gctgatcgtg ccgatgatga tcacgccggt cgtcgcggcg ttctgctgga
     1501 agctgctgct cgaccccgat cacggggtca tcaacttctt cctgcagcag cacattgtct
     1561 ggctgggccg gcctgatacg gccctgcttt cggtgtcggt ggtgaacatc tggcagaacg
     1621 ccccctacgt ctcgatcctt ttgttggccg ggttgcgctc gctgccgcat gaaccgatgg
     1681 aggccgcacg gatcgacggc gccagccggt tgcagatgtt ctggcacata accctgccgc
     1741 tgctgcggcc ttatttgctg gtcgccctgc tgctgcggat gattttcgag ttccgctcct
     1801 tcgacaacgt ttacgtcatg accagcggcg gccccgccaa cgccacgatg atgctgtcga
     1861 tgtacaccta cctcgcgtca ttcgtgcggt tcgacctcag cctgggtgcc gcggcgtcat
     1921 ggatgatgct gctgatctcc ttgatcatgt gcggctgctt catcgcgatg gtccgccggc
     1981 gggacgccaa ctgatggccg cggcgggacg ccgacaaatg gccgcggcgg gacgccgacg
     2041 cgcggcgggg cgcaagcgca tggaggcgct ggcggtttat gtggccctca gtgtgttgac
     2101 cgtcgcattc ctgttcccgc tgctctggat cggtggtctg tcgttgaaaa cacggctgca
     2161 agtgtttgcc tcgccgccat tgttcctgtg gtggccaacg ttcgagaact acgccgccac
     2221 gctatcgcgt gccgatttcg gacaggcatt cgtcaacagc ctgctgatct cggcaggagc
     2281 cgtttcgttg tcgctgctcg taggcgtgcc tgccgcctat gcgctggctc ggttcagttt
     2341 cgcgggccgg aactttctgt ttttcgccct gctgtcgatg cggatgctgc cgcccatcgc
     2401 cgttctggtt ccgatgtacg tgctgttcag cgcgttcggc ctgaccacga cccggacatc
     2461 ggtggtgctc gcctacacca ctttcagcct gcctctggtg gtctggatca tgcgcggctt
     2521 cttcgaggat ctgccgcgtg aacttgagga atccgcctgg gtcgatggcg ccagccgcct
     2581 gcaagccttc gtccacgtcg tgctgccgct gatcaagccc ggcctggtgg cggcctgcat
     2641 cctgtgcctg caacttgcct ggaacgattt tctgttcgcg gctgtgctca ccaacaatgc
     2701 cactcgcacc ttgcccgtgc tgatggcggc attttccgga ggcgataccg gggtcgattg
     2761 gggcgggatg accgcgtccg gcgtcctggt gatcctgccg gtcatcctgt tctcgttcgc
     2821 ggcgcaacgc catctggtag ccgggctatc ctccggcgcg gtcaaggggt aatccgaatg
     2881 acagggctga acagacgcca attgctggct gcctcggctg catcgacact accattgccc
     2941 gcgctggggc agggcaagcc ggacaaattg gtctatgtcg gcgacaacgg cccctggcac
     3001 tgggctttgg tcgaagaggt cgcgccggct ttcgaaaaag ccaccggcat caagatcgac
     3061 tttacccttc tgcccgtcga tccctggact gcccgattga aggccgaact tagttcgggg
     3121 tcgagcggca tcgacatcgt gcaatggtcg gtcggcatgg ccggctggat cagcccgcac
     3181 atgctggacc acgaggtcgt ggccagcgag atcatggcca aagatccgtc ctggaactgg
     3241 gacgatttcc tgtccggcag caaacgggcg gccacctacg acggcaagat ggccggcatt
     3301 ccgtatcgcg tcactaccgg cgtcatgcac taccagaagg cgctgctgga gcaggcagga
     3361 atcacgaaat tgcctgaaac cttcgccgaa ctggaaaagg ccgcgatcgc cgccaacacc
     3421 ccgccgcagc gctatggctt tggcatctcc ggacgccaag ggccggcgat cctaagcagc
     3481 ttcctgccat ggctgtattc ctgcggcggc aacctgttgg atttcaaaac cggggagatc
     3541 ttgatcaaca acgaaaaagg cgtcgcggca ctggatttct ggaccggact tatccttaag
     3601 gacaaggtcg tgccgcccga ggcgatgacc tgggaatatg acgagatcgt cgccggcggc
     3661 caaaacgacc gctacgctat ggtgcagacg ttcgcgccct atggcacgct gatcaacgat
     3721 ccaaagatct ccaagaccgg aggcaaatgg acctgggcca cggtgcccgg ccagacggcg
     3781 aaggatcagg gccgtacctg ggtcgatggc cacttcatgg gtatcccaaa atacacgcgg
     3841 aacaaggact ggtcgctggc attcatccag atggcgtgca gcaaggattg gatgcgccgg
     3901 tcgatggtgc gcggcaacgc gcctccccgc ggctctgtgc tgcgcgatcc ggagatggtg
     3961 gagacgatcg gctggccgcc aaccgcggcc accgcgatcg agaccggctt tccgacaccc
     4021 gcccaaccgg tctggagcac gttgcagcta tcgctgcgca cgggcttgtc acaggcggtt
     4081 ttgggacaga aaaccgcgaa gcaggcgctg gacgatgtag ctgtggattg gcagcgcggc
     4141 ctgaggcgag cgggagtggg caagggatga agcgccgaag tcttctcaca gctggcgtcg
     4201 cgctggcact gccttcggtg gcgcgggcgg gcgccaagcc cgaaaaactg gtgttcgtcg
     4261 gcgacaacgg accatggcac tggtgcctgg tggaggaagt cgcgccggcc ttcgaaaaag
     4321 cgaccggcat caagatcgac ttcaccctgc tgccgatcga tgcgctgact gctcggttga
     4381 aaggggagct gaactccggt tcgccgaata tcgacatcat ccagtggaca gccccgttcg
     4441 ccgggtggct ggcgccacat atggcggatc attcgaaact gctggcggac aatgccgggc
     4501 ggcatcccga tttcgattgg gacgacttcc tgccgtcggt gcgcgacatg gccagctacc
     4561 agggccagtt gctgggcatc ccctaccggg tgacgaccag catcctgaac taccagaagc
     4621 agttgctttc ggatgcgggt tttgccacgc cgccggacaa ctgggacagc ttcctggcgg
     4681 cggcgcaagc cacgaccaag ccgcctgagc gctacggcct gg
//
LOCUS       DASWQN010000006         3581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1452901, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000006 DASWQN010000000
VERSION     DASWQN010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452901"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VGC82_00070"
     CDS             complement(<1..250)
                     /locus_tag="VGC82_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0421701.1"
                     /translation="MLDIAFAKPALPKSGALVLLMHEGEEPSGLWAQLDEATGGAIAR
                     AFVVAEFKGGKGKNCMILAPGANITRVLAVGLGKPDEVT"
     gene            complement(355..1977)
                     /locus_tag="VGC82_00075"
     CDS             complement(355..1977)
                     /locus_tag="VGC82_00075"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY0421702.1"
                     /translation="MLAETDTYDFIVTGAGSAGCVLAARLTESGRYKVLLLEAGGKDT
                     NPWIHIPMGYAKTFVDPKVNWKFESEPEKELNGRTMYQPRGKVLGGTSSINGMIYMRG
                     NAADYDQWRQLGNEGWDYASVLPYFRKAEDNERGSDDFHGSGGPLRVSNQPYEWEIAK
                     VLLEACKQAGIPFNPDFNGAKQEGCGYYQTTTRDKRRWSTAAAYLRTAKRRPNLTVKT
                     RAHATRVLFDGNKAVGVEFRTPRGIEVARAGREVIVSGGAYGSPHLLQLSGVGPAQHL
                     TDLGIDVVRDMAGVGSNLQDHFNTYCTYRISKNLSLNALHYSLSHRLLAGAQYLFLRS
                     GPMSGNGMYVGALVRSDKRLERPDLQFNISAWSTIDRNATGIISHPFPGISISPVHLA
                     PEGRGTVRLKNRDPFAPPEIKFNFLRNESDMRVMIAGVRIARDIARQHALQKLMVEET
                     APGVATRTDQEIAEDVRRRGVSNLHPVGTCGMGQGPMAVVDPRLRVHGIAGLRVVDAS
                     IMPVIVAGNTNAPTIMIAEKASDMIQEDARAA"
     gene            complement(2052..>3581)
                     /locus_tag="VGC82_00080"
     CDS             complement(2052..>3581)
                     /locus_tag="VGC82_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0421703.1"
                     /translation="SGRYRVLLLEAGPPDKNPWIHIPLGFAKTYVDSAVNWKFETEPQ
                     PQMQNRRLYLPRGKTLGGSSSINGMVYIRGNHADYDEWRQRGCTGWDWDSVLPFFKKA
                     ENQTRGADAFHGVGGPLHVSEQAEKGELSRAMLEACVQAGIPANPDFNGAQQEGCGYY
                     QTTSSNKRRWSAAKAYLSNPPANLTIRTNAHATRVLIDKGRATGVEYQTGAGRQVSSA
                     GREVIVSGGAYGSPQLLLLSGLGPAQHLQDMGIQVVRDMPAVGSNLHDHFNTVVSWRC
                     SKPITLNDLQNSSLRKLAAGVRYGLFRSGPMASNGVTAGLFTRSDPRLERPDIQVNLF
                     EWSTKERSRNAVIAHAFPGFTLTPVHLRPDGRGTVRLGSPDPFAWPAVLFDYLRTEYD
                     IGAALTGLRLVRRIADQPALRPYVVNEIYPGVDVTSDDDLLDYVRRTGVSNQHPTSSC
                     AMGTGTNSVVDPRLRVHGIAGLRVADASIMPVVVGGNTNAPTIMIGEKAAAMILEDAT
                     A"
BASE COUNT          621 a         1166 c         1091 g          703 t
ORIGIN      
        1 gcgtaacttc gtccggtttc ccgagcccga ccgccagaac ccgcgtgatg ttggcgccgg
       61 gcgccaggat catgcagttc ttgcctttgc cacctttgaa ctcggcaact acgaacgcgc
      121 gcgcaatcgc cccgcctgtg gcttcatcga gttgcgccca cagcccagag ggttcctcgc
      181 cctcatgcat cagcagcacg agcgcgccgg atttgggcaa tgccggcttg gcgaaggcaa
      241 tatcgagcat ttaaatgatg tctccagtcc gtcgtcagtc caggatagtg cgtttcgggg
      301 gccgctgaaa gcaaccggtc aatgccttag cctttgcggt gcccccggca cgcttcacgc
      361 tgcccgggca tcctcctgga tcatatccga tgccttctcc gcgatcatga tcgtcggcgc
      421 gttggtgttg cccgcaacga tcacgggcat gatcgacgca tcgacgactc gtaacccggc
      481 gatcccatgc acgcgaagac gcgggtcaac gaccgccatt ggcccctggc ccatgccaca
      541 cgtgccgacg ggatgcaggt tcgacacgcc acgccggcgc acgtcctcgg cgatttcctg
      601 atcggtgcgg gtggcgacgc cgggtgcggt ttcttccacc atcagcttct gcaacgcgtg
      661 ctgccgagca atatcccggg cgatacgcac ccccgcgatc atcacccgca tgtcgctttc
      721 gttgcgcaag aagttgaact tgatctcggg cggagcgaac gggtcccggt tcttcaggcg
      781 gaccgtgccg cggccttcag gtgcgaggtg cacggggctg atggaaatcc ccgggaacgg
      841 gtgggatatg atccccgtag cattgcggtc gatcgtgctc caggcggaga tgttgaactg
      901 cagatccggc cgttccagac gcttgtcgct acgaaccagc gcgccgacgt acatgccgtt
      961 cccggacatc gggccggagc ggaggaacag gtactgcgcg ccggccagca atcggtgcga
     1021 caggctgtag tgcagcgcat tcaatgacag gttcttcgag atccggtagg tgcagtaagt
     1081 gttgaaatgg tcctgcaggt tggagccgac gccggccatg tcgcgcacca cgtcgatgcc
     1141 caaatccgtc aaatgctgcg ccgggcccac gccggataat tgcagcaagt gtggagaacc
     1201 atacgccccg ccagatacga tcacctcgcg tccggcacgg gccacttcga tcccccgggg
     1261 cgtgcgaaat tccaccccga cggccttgtt gccatcgaac agcacacgcg tggcgtgggc
     1321 ccttgtcttg accgtcaaat taggccgccg cttcgccgtg cgaagataag cggcggccgt
     1381 gctccagcgg cgcttgtccc ttgtggtggt ttgatagtag ccgcagcctt cctgcttggc
     1441 gccattgaag tcggggttga acggaatccc ggcctgcttg caggcttcca gcaatacctt
     1501 ggcgatttcc cattcgtacg gctggttgga cacgcgcaac ggcccgccgg agccatgaaa
     1561 atcatccgaa ccgcgctcgt tgtcctcggc cttgcggaag tacggcagga ccgaagcgta
     1621 gtcccagcct tcgttgccaa gctgtcgcca ctgatcgtag tcggccgcgt tgcctcgcat
     1681 gtagatcatg ccgttgatgg aactggtgcc gcccagcacc ttgccccgag gctggtacat
     1741 cgtgcggccg ttcagctcct tctctggttc ggattcgaat ttccaattta ctttcggatc
     1801 gacaaaggtc ttcgcatacc ccatcgggat gtggatccag gggttggtgt ccttgccgcc
     1861 ggcctccagc agcaggacct tgtaacgccc ggattcggtc agccgcgcgg ccagtacgca
     1921 ccccgccgat cccgcaccgg tcacaatgaa atcgtaggtg tctgtttccg ccagcatgtt
     1981 tctttccccg tccgttccgc tggagattac cccgcccgct cggaagcgcc accccttgcg
     2041 cggccagcgg atcaggccgt ggcgtcttcc aggatcatcg cggcggcttt ttccccaatc
     2101 atgatcgtgg gtgcgttggt gtttccgccg accacgaccg gcatgatgga cgcgtccgcg
     2161 acccgcaagc cggcgatgcc atgcacgcga agccgaggat cgacaacgct gttcgtgccg
     2221 gtccccatgg cgcaggaact tgtcgggtgc tggttggaaa caccggtccg gcgcacgtag
     2281 tccaacagat cgtcgtcgct ggtaacatcc acgccgggat aaatttcgtt caccacgtac
     2341 ggccgcaacg ccggctggtc ggcgatcctg cgcaccagcc gcaagccggt cagcgctgcc
     2401 ccaatatcgt attcggtgcg caagtagtcg aacagaaccg cgggccaggc aaacgggtcc
     2461 gggctgccaa gccggacggt gccccggccg tcaggacgca gatgcaccgg cgtcaaggtg
     2521 aagccgggaa acgcatgtgc gatcacggcg ttgcggctgc gttccttggt gctccattcg
     2581 aacaggttca cctggatgtc gggccgttcc aatcgtgggt cagatcgggt gaacaggcca
     2641 gcggtaacgc cgttgctggc catcggtccc gagcggaaca ggccgtatcg caccccggca
     2701 gcgagttttc gcagcgaact gttttgcagg tcgttcagcg tgatcggctt gctgcaccgc
     2761 cagcttacga cagtgttgaa atgatcgtgc aggttggacc ctacggccgg catatcgcgc
     2821 accacctgga tgcccatatc ctgcaagtgc tgcgccggcc caagtcccga cagcagcagc
     2881 aattgcggcg agccgtaagc accgccggat acgatcacct cccgtccggc gctgctgacc
     2941 tggcgcccag ccccggtctg gtattcgacg ccggtggcgc ggcccttgtc gatcagcacg
     3001 cgggtcgcat gcgcgttggt ccggatggtc aggtttgccg gaggattgct gagatacgcc
     3061 ttggccgcgc tccaccggcg tttgttgctg gacgtggtct ggtagtagcc gcaaccctcc
     3121 tgctgggccc cgttgaagtc ggggttcgcc ggaattcccg cctggacgca ggcttccagc
     3181 atagcgcggg acaactcgcc cttttccgcc tgttcggata cgtgcaacgg gccgccgacg
     3241 ccatggaacg catccgcgcc gcgggtctgg ttctcggcct tcttgaagaa cggcagcacc
     3301 gaatcccagt cccagccagt gcatccacgc tgccgccatt catcgtaatc ggcgtggttg
     3361 ccgcgtatat acaccatgcc attgatagag ctggagccgc caagggtctt gccccgaggc
     3421 agatacaacc tccgattctg catctgcggc tgcggctcgg tttcgaactt ccagttcacc
     3481 gccgaatcga cataggtctt ggcaaacccc agcgggatat gaatccatgg attcttgtcc
     3541 ggcggcccgg cttccagcag cagcacgcgg tagcggccgg a
//
LOCUS       DASWQN010000007        10025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1848795, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000007 DASWQN010000000
VERSION     DASWQN010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 10025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10025
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848795"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2135)
                     /locus_tag="VGC82_00085"
     CDS             complement(<1..2135)
                     /locus_tag="VGC82_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEY0421704.1"
                     /translation="MTELAAVSLEVVFDAIPVGLGIVDPSGRIILMNRAFRDSLALPL
                     DAFPPGTLVVDAVRASALRGVYGPGDPEAQVSAIMAADRSRPGRLRRRTFAGRSHDLY
                     NTPLPDGGYVVSAVETTAWQDANAEAEAALAQTSTALAASRIGLAVFDLQRRLVLANP
                     RFAALLALPPDRIGAGTSFDGMLALMQTREAFASPDGTAFIASLREAASGRAGTIRHR
                     RADGRSIDIMFDPLPDGGCTIAVSDITAQARAEDDAWRRARLLDLVLLNVPHGICVYG
                     PDHRVAMFNETYNKVMAGAPLQIGDSLSDVIHRRAEAGEYGAGAPEAVIAAQMAHDIS
                     RPQMRRRLRPNGTAIDIRTAPLPDGGHISVVTDISALVQAEAELRRHAEDMSTMLDNI
                     RHGIMLWGSDRRLIASNPVAADLLDLPADLLIPGRAEAEVIDKISELGRFGAGEPAPL
                     ARKLLDLDRSVPFGREIATPSGRILYAQSNPSPDGGWITTFTDITRMRQTELELRRAK
                     EQAEAANLAKSRFLATMSHELRTPLNAIIGYSDAMVRENADVPAALVAEYSGQINEAG
                     KQLLSLINVILDVARIESGQFEPGGDIVDIGKAVHGAVRQVDAAATAGQVSIDLTLAD
                     DLAALHGDERQITQAVAQLLSNAVKFTDAGGSVTIKAGLTAEADVFVSVADTGIGIPE
                     IELERAFEPFTQLDASLSRRYSGTGLGFS"
     gene            complement(2132..3247)
                     /locus_tag="VGC82_00090"
     CDS             complement(2132..3247)
                     /locus_tag="VGC82_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY0421705.1"
                     /translation="MPFDVRDFPNASRKDRAATPQSRRLVAGWLFTVAAMIMVMILLG
                     GATRLTGSGLSIMEWAPLMGTLPPFSDAEWQRLFALYQKIPQYTLVHEGFGLAGFKQI
                     FWLEWTHRLWGRLIGVVFLLPMLWLWATGRIERRLLPRLALLFVLGGLQGAIGWFMVA
                     SGFLPDATAVSPYRLVIHLVLALVLYAAIVWTGMSALYPHRHKAAVPLMVRGLAIVSL
                     TMVSLTIVAGGFTAGLHAGLTYNTFPLMDGHLIPDGYAMLHPLIRNLTENVAAVQFDH
                     RLLATMTLIVVSALAAAGWRSGSLRWQMGCLGFAVVAQYILGVTTLLLVVPVSVATLH
                     QFGAVILLTTVLVLVHRLGNAAHRPEPALPPAETAVT"
     gene            complement(3334..4233)
                     /locus_tag="VGC82_00095"
     CDS             complement(3334..4233)
                     /locus_tag="VGC82_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0421706.1"
                     /translation="MPSLPATVADTAKLLSENGYIADLELATTVHLALAMQRPLFLEG
                     EPGTGKTEIAKVLATGLKRRLVRLQCYDGMDLSAAAYEWDHARQLMAIRLAEAAGHTD
                     RDELSRDIYTREFLQARPLLSAIDPDLPPAVLLIDELDRADEPFEAFLLELLADFQIS
                     IPEFGTVRAVTRPLVILTSNRTREVHDAIRRRCLYHWVDYPDASRERAILATRAPGVP
                     EKLAQQVVGFVQRLRGEELFKLPGVAETIDWAQALTYLNKTELTAGDVDDTLGVLLKY
                     QDDIAKVRGAEAARLITEVAASG"
     gene            complement(4233..4901)
                     /locus_tag="VGC82_00100"
     CDS             complement(4233..4901)
                     /locus_tag="VGC82_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEY0421707.1"
                     /translation="MDMTGERRIPAPRQTVWEALNDPDVLKASIPGCESLEKLADNQM
                     KATAAVKVGPISARFTGKVQLTDIDPPNGYRISGEGQGGVAGFAKGGANVTLADDGAD
                     TLLSYQVNAQVGGKLAQLGGRLIDATAKQMADAFFDRFSKQVQALSPAPDASRPPNVA
                     AEGAVSKPGAETRSTATSPAHEPVSVSFWSLIPKEAFGLPLAFWLGGGVFAVMVLLML
                     GSML"
     gene            5005..5604
                     /locus_tag="VGC82_00105"
     CDS             5005..5604
                     /locus_tag="VGC82_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421708.1"
                     /translation="MAKNALRPLAFPALDRLAALDRLAALDGEGQPGDHCPEGGTDTK
                     GWIAKMGRWPRLVLLGLAVLDCLATPRPAGAREAVTQKSCRTITQAQVGALFDQWNRA
                     LATKRTEAVVAEYAPYATLLPTVQNGPLTGAEAIGRYFAHFLKQSPQATIETRVIHTG
                     CNIAYDIGLYSFMVDGNQPGARKQVKARYTFVYAPVHGM"
     gene            complement(5644..6408)
                     /locus_tag="VGC82_00110"
     CDS             complement(5644..6408)
                     /locus_tag="VGC82_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0421709.1"
                     /translation="MIRIGTAGWTIPKPDRAAFPVQGTHLERYGQRFAAVEINSSFYR
                     PHKLDTYTRWAASVPPDFRFAAKLPREVTHARGLIAAAEPLERFLSEIKGLGDRLGPL
                     LIQLPPALSFNEASVGLFFQEFRSRFDGLAVCEPRHASWFTDRVEALLVRYRIARAAA
                     DPAPVPKARTPGGWPGLIYRRLHGSPRMYYSAYPPEYIEATAALMGEIPDRDREHWCI
                     FDNTALGEATGNALALKRRLEQAPMPPANVPSARLL"
     gene            complement(6435..6674)
                     /locus_tag="VGC82_00115"
     CDS             complement(6435..6674)
                     /locus_tag="VGC82_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421710.1"
                     /translation="MPHLSNSFAALGPPQGEEAQQRLKKGLTFLKHRVFLSVRSGIVA
                     AGLTIRDYMTIRRLYDDRPARFIRRVHEAHGARPQ"
     gene            6728..7450
                     /locus_tag="VGC82_00120"
     CDS             6728..7450
                     /locus_tag="VGC82_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY0421711.1"
                     /translation="MISACCAFPAWAGAPFVTDDADTPEANHFEINLAAQYTKFQGGS
                     VGAIPSLEVNYGITDKLQVAILTPLAMAQTDGSGTNIGLGDVELGVKYRFIDSDDWGW
                     RPGIAFAPSIVMPSGSEARGLGSGQIQAFLPIWLSKEFGQWTVFGGGGYNINPGSDRL
                     NWWFTGIGVARELSPKWTVGAEIFHATPTDRGAKDDTAFNVGAIYNISDTHHLMMSIG
                     RNIINAKENNALSVYIGYQLTL"
     gene            7514..8038
                     /locus_tag="VGC82_00125"
     CDS             7514..8038
                     /locus_tag="VGC82_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421712.1"
                     /translation="MTNKRIFGAGALILAMSISSVGARAFDMAGFKSRLETTKAETVT
                     KTLTDSKATLARLDEMIKIGVVGAKEYADKQPAFAKLMAAVIADADPMKGLSDAEIEA
                     KWGEQGTGGDALGIPLKSLGQFDDTRAAVELIVSPAHAYIYVKKWESAQKARWLDQAR
                     DELNELSEHLKQVR"
     gene            8119..9849
                     /locus_tag="VGC82_00130"
     CDS             8119..9849
                     /locus_tag="VGC82_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0421713.1"
                     /translation="MLTRATRSVRNVSITKKSVAVFASLLIVVLGLGAQSILRLGMVK
                     TAGDTISARWMPGVQRAGEIAEAANTFRDAESTMVLTPDDDSVSEMKKSLALSLKQIE
                     AARAALATMPMTTEEAGFIGEFNDTWNAYLTLSNRIVEMVAQKKIADAADLLTGDSAY
                     QFTKAKNFAGRLVVAKVKGGDDAIAGAAALYQATIPLMAGAIVVAMLLCLGAGAFVIA
                     GVARPLTSMSRVVRRMAGGELDIPDDDMPGLGRGDEFGALAQALSVLRDSARARVRLE
                     REAAESRALADTRQSEMERHTQDFGLSVSGVMVMLSKAAEGISDSAAIMTRTADETRS
                     QADATASNSLRAAESLSAVAAAAEEMAVTASEVGRRIGDVTTSTDAAVEAAVRSEKIV
                     TALVGAVAEIGSVVQLIGDIAGQTNLLALNATIEAARAGDAGKGFAVVASEVKMLAMN
                     TRKATEEVNTRIMSVRASTTEAQQAIEGVTTAIGRVHEAAAEIAASIEQQSLATREIA
                     SSVQNVSIQTDGTTRAMSGLSVVADETSSASKEVLRAAGQVREQTVTLRQEVGSFLAA
                     TRAAGGDRRP"
BASE COUNT         1981 a         3112 c         3215 g         1717 t
ORIGIN      
        1 catgaaaaac ctaatcccgt ccccgaatat cgccgcgaca gcgatgcatc aagctgcgtg
       61 aacggttcga aagcccgctc cagctcgatc tccggaatcc caatgccggt atcggcgaca
      121 gaaacgaaaa catccgcctc agccgttagt cccgctttta tggtgaccga accacccgca
      181 tcggtgaatt tgacggcgtt cgacagaagc tgtgccacgg cctgggtgat ttgccgctca
      241 tcgccgtgca gcgcagcaag atcgtcggca agcgtcagat cgatcgagac ctgcccggcc
      301 gtcgccgcgg catccacttg ccgcacggcg ccgtgaacgg ccttgccgat gtcaacgatg
      361 tcgccaccgg gctcgaactg gccgctctcg atgcgcgcca catccaggat gacattgatc
      421 agcgacagga gttgctttcc ggcctcattg atctggccgc tatattccgc caccagcgcc
      481 gccggcacgt cggcgttttc acgcaccatc gcgtccgaat agccgatgat cgcgttcagc
      541 ggcgtgcgaa gctcatggct catggtcgcg aggaaccggg atttcgccag attcgccgcc
      601 tctgcctgtt ccttcgcccg ccgcagttcc agttccgtct gccgcattcg ggtgatgtcg
      661 gtgaaagtgg taatccatcc gccatccggc gaggggttgg attgggcata gagaatgcgg
      721 cctgatggcg ttgctatctc acggccgaac ggcacggaac gatcgaggtc gagaagcttg
      781 cgtgccaagg gagcgggttc gccggcgccg aaacgcccaa gttcggaaat cttatcgatc
      841 acttcggctt cggcccgacc ggggataagc agatcggccg gcaaatccag aaggtcggca
      901 gcaaccgggt tgctcgcgat caatctccgg tcggaacccc acagcatgat gccgtgccgg
      961 atgttgtcca gcatcgtgct catgtcttcg gcatgccggc gtagttcggc ctccgcctga
     1021 accagcgcgg agatatccgt cacgacgcta atatgacccc cgtccggcag cggcgcggtg
     1081 cggatgtcga tcgcggtgcc gttcggacgc agccgccgcc gcatttgtgg gcgtgagata
     1141 tcgtgcgcca tctgcgcggc gataacggct tccggggcgc ccgcgccgta ttcgcccgct
     1201 tccgcacgcc ggtggatcac gtccgacagc gagtcgccga tctgcagcgg cgcgccggcc
     1261 atcaccttgt tgtatgtctc gttgaacatc gcgacccggt ggtccgggcc gtaaacgcaa
     1321 atgccatgcg gcacattcaa cagcaccaga tccagcaagc gagcccgccg ccatgcgtca
     1381 tcctccgctc gggcttgcgc tgtgatgtcg ctgaccgcaa tggtgcaacc accgtcgggc
     1441 aacgggtcga acatgatatc gatcgagcgg ccatcggccc gcctatgccg gatggtcccc
     1501 gcacggccgg atgcggcctc gcgcaaggaa gcgatgaacg ccgtgccgtc cgggctggcg
     1561 aaggcttcgc gggtttgcat cagcgccagc atgccgtcaa aactggtgcc ggccccgatc
     1621 cggtccggcg gcagcgccag caaggcagcg aacctggggt tggccagcac cagccggcgc
     1681 tggagatcga ataccgccag gccgatccgc gatgccgcca gtgcggtcga ggtctgcgcc
     1741 agcgccgctt cagcctcggc attcgcgtcc tgccaagccg ttgtttccac cgcgctgacc
     1801 acgtagccac cgtccggtaa cggggtgttg taaaggtcgt ggctccggcc cgcgaaggtt
     1861 cgccggcgta aacggcccgg ccggctgcga tccgccgcca tgatcgccga cacctgtgcc
     1921 tcgggatcgc ccggaccata aacaccgcgc aaggccgagg cgcgcacggc atccaccacc
     1981 agcgtgccgg gcgggaaggc atccagcggc agtgcgagcg agtcccggaa cgcccggttc
     2041 atcaggatga tgcgacccga cggatcgacg atgccaaggc caacggggat cgcgtcgaac
     2101 acgacctcca gagagaccgc ggccagctca gtcatgtcac cgccgtttcg gccggcggca
     2161 gtgctggttc cggccggtgt gcggcattgc caaggcgatg caccagcacc agcaccgtgg
     2221 tcagcaggat gactgccccg aactggtgca gtgtcgctac ggataccggg accaccagca
     2281 aaagggtcgt cacgcccagg atgtattggg ccacgaccgc gaaaccgagg catcccatct
     2341 gccagcgaag cgaacccgag cgccagcccg cggccgccag ggcggaaacc acgatcaaag
     2401 tcatcgtcgc cagcaagcgg tggtcgaact gcaccgccgc gacgttttca gtcaggttac
     2461 ggatcagcgg atgcagcatc gcgtagccat ccgggatcag gtgcccgtcc atcaggggga
     2521 aggtgttgta cgtgagtccg gcgtgcagcc cggcggtgaa accaccggcc acgatcgtca
     2581 ggctgaccat ggtaagcgaa acgatcgcca gcccgcgcac catgagggga accgccgctt
     2641 tatgccggtg cggataaagg gccgacattc cggtccaaac gatcgcggca tacagcacca
     2701 gcgccagcac cagatggata accagccgat acggcgaaac cgcggtcgca tcgggcagga
     2761 aaccggaggc aaccatgaac cagccgattg ccccctgcag tccgcccaga acgaacaata
     2821 gggccaggcg cggcagcagc cggcgctcga tccggccagt ggcccacagc cagagcatcg
     2881 gcagcaggaa cacgacaccg atcagcctgc cccataagcg gtgggtccat tccagccaga
     2941 atatctgctt gaatccggcg agaccgaagc cctcgtgcac cagcgtgtac tgcggaattt
     3001 tctggtagag cgcgaacaag cgctgccatt ccgcatcgct gaatggtggc agagttccca
     3061 tcagcggcgc ccattccatg atcgacaggc cggagccggt caatcgtgtg gcgccgccga
     3121 gcaggatcat caccatgatc atggccgcga ccgtgaacag ccagcccgcg acgaggcggc
     3181 gggattgcgg cgtcgcggcg cgatccttgc gggaggcatt cgggaaatca cgcacatcga
     3241 atggcatgga ccgagtatag agcctgggcg aatattaaag aagcatttcg cggtagaacc
     3301 gctcggtgct ccagtttggc gtcggccaaa ggtttagccc gaagcggcga cctcggtgat
     3361 cagccgggca gcctcggcgc cgcggacttt cgctatatcg tcctggtatt tcaacagcac
     3421 gccgagcgtg tcgtccacgt caccggcggt cagttcagtc ttgttcaggt aggtcagcgc
     3481 ctgcgcccag tcgatcgtct ctgccacgcc cggaagtttg aacaactctt ccccccgcag
     3541 ccgttgcacg aagccgacca cctgttgtgc cagcttctcc ggcacgccgg gcgccctggt
     3601 ggccaggatc gcccgctcgc ggctcgcatc ggggtaatca acccagtgat acaggcagcg
     3661 gcggcggatc gcgtcatgta cctcgcgcgt gcggttggag gtcaggatca ccaacggacg
     3721 cgtgacggca cggacagtgc cgaactccgg aatggagatc tggaaatcgg ccagcagctc
     3781 cagcaggaag gcctcaaacg gctcgtctgc ccggtcgagt tcgtcgatca gcagcacggc
     3841 gggaggcaga tccggatcga tcgcggacag cagcgggcgg gcctgcagga actcccgtgt
     3901 gtagatgtcg cgggacaatt cgtcgcggtc ggtgtgcccg gcggcctcgg ccaagcgaat
     3961 ggccatcaat tgacgggcgt gatcccattc gtaggctgcg gcggacaggt ccatcccgtc
     4021 gtagcattgc aggcgcacca accggcgctt caggccggtc gccaacacct tggcgatctc
     4081 ggtcttgccg gtcccgggct caccttccag gaacaaggga cgctgcatgg ctagtgccag
     4141 atgcaccgtc gtcgccagtt cgaggtccgc gatatagccg ttctcagaga gcagcttcgc
     4201 agtgtcggca accgtggcgg gaagcgacgg cactagagca tgcttcccag catcagcagc
     4261 accatgacgg caaacacgcc cccaccaagc cagaaggcga gcggaaggcc gaaggcttcc
     4321 ttcggtataa gggaccagaa cgagaccgag acgggctcat gggccggaga agtcgcagtg
     4381 gagcgggttt cagccccagg tttggacact gcgccttcgg cggcaacgtt gggcgggcgg
     4441 cttgcgtccg gcgccgggga caacgcctgc acctgtttac tgaaccggtc gaagaacgcg
     4501 tccgccattt gcttcgcggt ggcatcgatc agccggccgc cgagctgcgc cagcttgccg
     4561 ccgacctgcg cattgacctg gtagctcagc agcgtgtcgg cgccatcatc cgccagcgtc
     4621 acgttcgcgc cacccttggc gaagccggcg acaccaccct gcccttcacc gctgatccga
     4681 tagccgttcg gcgggtcgat gtccgtaagt tggacttttc ccgtgaatct ggcggagatc
     4741 gggccgacct tgaccgcggc ggtcgccttc atctggttgt cggccagctt ctccaggctc
     4801 tcgcacccgg gaatgctggc cttcagcacg tccggatcgt tgagtgcttc ccaaacggtc
     4861 tggcgcggtg ccggaatgcg ccgctcgcct gtcatgtcca tacgaaaacc accccttact
     4921 gagcgcggcg gacgttaccg gtagggtccg agccatgcaa ccgacccatc ccgggccgtt
     4981 cgggcaggcc tgggcctgca tggcttggcg aaaaacgcgc tgaggccctt ggcgttcccc
     5041 gcgcttgatc ggctggcggc gcttgatcgg ctggccgcgc ttgatggcga ggggcaaccg
     5101 ggcgatcatt gtccggaggg cggaacggat accaaggggt ggatagcgaa gatgggacga
     5161 tggcctcgac ttgtgctcct gggattggcg gttttggatt gtctcgcaac gccaaggccc
     5221 gcgggtgcgc gagaagcggt tacgcagaaa tcgtgccgga ccattaccca ggcgcaggtc
     5281 ggcgccctgt tcgaccagtg gaaccgcgcc cttgcgacga aacggacgga agccgtggtc
     5341 gcggaatacg cgccctacgc aacgctgttg ccgacagtgc agaacggccc gctgaccggt
     5401 gcggaagcga tcggccggta tttcgcccat tttctgaagc agtctccgca agcgacgatc
     5461 gagaccaggg tcattcatac cggatgcaac atcgcctatg acatagggct ttattcgttc
     5521 atggtggatg gtaaccagcc aggggcccgc aagcaggtga aagcccgcta tacattcgtt
     5581 tatgcgccgg ttcacggcat gtgactgatt gtccaccacc actcctcggc cgacccggtg
     5641 gcttcacaat aggcgggctg acggcacgtt cgctggcggc ataggggcct gttccagccg
     5701 tcgcttcagg gcaagcgcat tgccggtagc ctcgcctagc gcggtgttgt cgaagatgca
     5761 ccaatgttcc cgatcacgat ccgggatctc gcccatcagg gcggcagtcg cctcgatgta
     5821 ttccggtggg taagctgagt aatacatgcg cggcgagccg tgcagccggc ggtaaatcag
     5881 tccgggccaa ccgccggggg tcctcgcctt tggcaccggg gccggatcgg cagcggcccg
     5941 cgcgatccga taccgcacaa gcagcgcttc gacccggtcg gtaaaccagg aggcgtggcg
     6001 gggttcgcaa accgccaggc catcgaatcg cgagcggaac tcctggaaaa acagtccgac
     6061 actggcctcg ttgaagctga gcgcgggagg caattgaatc agaagcgggc ccaggcgatc
     6121 gcccagcccc tttatctccg atagaaagcg ctcgagcggt tcggctgcgg cgataagccc
     6181 gcgagcatgg gtgacttccc gaggaagttt ggcggcaaag cgaaaatccg gcggaacgct
     6241 cgcggcccag cgcgtgtagg tgtccagctt atgcggccgg tagaacgagg aatttatttc
     6301 gaccgccgcg aaccgctgtc catagcgctc gaggtgagtg ccctgaaccg gaaaggccgc
     6361 gcgatccggc ttcgggatgg tccagccggc ggttccaatc ctgatcatgg ctggggccct
     6421 ggctacctcc gtcgtcactg tggcctggct ccatgggctt catgcacgcg gcggatgaac
     6481 cgcgccggac gatcgtcata taggcgccgg atcgtcatgt agtcccggat cgtcaaaccg
     6541 gcagcgacga tacccgaacg aacggacaga aaaacacgat gtttgaggaa ggttaacccc
     6601 tttttaagac gttgctgcgc ttcttcgccc tgcggcggcc ccaaagccgc gaaggagttc
     6661 gaaaggtgcg gcaatggctt tgatcacgtc aagacgacct aaatgcacag gccttttggc
     6721 atgggcggtg atatcggcct gttgcgcttt cccggcctgg gccggagcgc ctttcgtaac
     6781 ggacgatgcc gacacccccg aagcgaatca tttcgagatc aacctcgcgg cgcaatacac
     6841 aaaattccag ggcggctcgg tcggcgcgat tcccagcctg gaggtaaatt acggcattac
     6901 cgacaagctg caggtcgcga tcctgacccc gctggcgatg gcccagaccg acggcagcgg
     6961 gacgaacatc ggccttggcg atgtcgaact cggtgtgaaa taccgcttca tcgactccga
     7021 tgactggggc tggcggccgg gaatcgcgtt cgcgccgtcg atcgtcatgc caagcggcag
     7081 cgaagcgagg ggccttggaa gtgggcagat ccaggcgttc ctgccgatct ggttatcgaa
     7141 ggaattcggt cagtggaccg tcttcggcgg cggtgggtac aatataaacc caggtagcga
     7201 tcgcctgaac tggtggttta ctggtattgg cgtcgcccgc gaactttcac cgaaatggac
     7261 agtcggcgct gaaatcttcc acgcgacgcc gacggatcgt ggcgccaagg atgacacggc
     7321 gttcaacgtc ggtgcgatct acaatatcag cgatacccat catctgatga tgtcgatcgg
     7381 ccgcaatatc atcaacgcca aagagaacaa tgctttgtcg gtctatattg ggtaccagct
     7441 taccctctga ctgcgagccc ttatcaatcc ctggccgtgg gcaagcggca ttcgcgcgac
     7501 tcaggagtaa tcgatgacga acaaacggat ttttggcgcc ggcgcgttga tactggcaat
     7561 gagcattagc tcggtcggcg cccgggcctt cgatatggcc ggcttcaaaa gccggttaga
     7621 aacaaccaag gcagaaaccg tcacgaaaac actgacggac agcaaggcga cgctggcgcg
     7681 gctggacgaa atgatcaaga tcggcgtcgt cggtgccaag gaatacgccg ataagcaacc
     7741 ggcgttcgcc aaactgatgg ccgctgtgat agccgacgcg gatccgatga agggcctgtc
     7801 cgacgccgag atcgaagcca agtggggcga gcaaggcacc ggtggcgacg cccttggcat
     7861 cccattgaaa tcgttgggtc agttcgacga tacgcgcgcc gcggtggagt tgatcgtaag
     7921 cccggcccac gcctacattt acgtcaagaa atgggagtcc gcgcagaagg ctcgatggct
     7981 ggatcaggcg agggatgagt tgaacgaact cagcgagcac ctgaagcagg ttcgataacc
     8041 tccgccccag cggccgcccg ccaggacaag gggcggctac agacctatct tgccacagcc
     8101 gaggctgcca gcatgaaaat gctcacacgc gcgacccgaa gtgtacggaa cgtatcaata
     8161 acaaaaaagt cggtcgccgt tttcgcgagc ctgcttatcg tggtgcttgg tcttggcgcc
     8221 caatccatct tgcgtttagg aatggtcaag acggccggcg acacgatcag tgcccgctgg
     8281 atgcccgggg tccagcgcgc cggcgagatc gccgaggcgg cgaatacctt ccgcgatgca
     8341 gaatcgacta tggttttgac accggacgat gattcagtat cagagatgaa aaaaagcctc
     8401 gcgctgtcac tcaagcaaat cgaggctgcc cgagcggcgc tcgccaccat gccgatgacc
     8461 accgaggagg cgggattcat tggtgaattc aacgacacct ggaacgctta cctgacactc
     8521 agcaaccgta ttgtcgaaat ggttgcgcaa aagaaaatcg ctgacgcggc ggatttgctg
     8581 accggcgact cagcctatca gttcaccaag gccaagaact tcgccggccg tttagtcgtt
     8641 gcaaaggtca agggcggtga cgatgccatc gctggcgccg ccgcacttta ccaagcgacg
     8701 atacccctga tggccggcgc tatcgtggta gcgatgttgc tgtgcctcgg tgcgggcgcc
     8761 tttgtgattg ccggcgtcgc tcggccattg acgagcatgt cccgggtcgt gaggcgtatg
     8821 gcaggcggag aactcgatat acccgacgac gatatgcccg ggctggggcg aggcgacgaa
     8881 ttcggtgcgc tcgcacaagc cttgtctgta ctccgggatt ccgcgcgagc gcgcgtgcgg
     8941 ctggaacgtg aagccgcgga atcgcgagcc ctggcggata ccaggcagtc ggagatggaa
     9001 cgccacaccc aggattttgg actgtccgtg tccggcgtca tggtgatgtt gagcaaggcg
     9061 gccgaaggaa tcagcgactc ggctgcgatt atgacccgca ccgcggacga aacgcggtct
     9121 caggcggatg cgacggcatc caattccctg cgcgcggcgg aaagcctttc ggcggtggcc
     9181 gcggcagccg aggagatggc cgtgaccgca agcgaggtcg gtcgccgcat cggtgacgtg
     9241 acaacgtcaa ccgacgccgc ggtcgaagcc gcggtccgtt cggaaaagat cgtgacggca
     9301 cttgtgggcg ccgtagccga aatcggatcc gtcgtgcagt tgatcggcga catcgcgggc
     9361 cagactaacc tgctggcctt gaacgccacc atcgaggccg cccgcgccgg cgacgctggc
     9421 aaaggttttg ccgtcgtggc cagcgaggtc aaaatgctgg ccatgaatac gcgtaaggcc
     9481 actgaagagg tgaacacccg gatcatgagc gtcagggcct cgaccacaga ggcgcagcag
     9541 gcgatcgaag gcgtcacgac ggccatcgga cgggtgcacg aggcagccgc agagattgcc
     9601 gcatcgatcg agcagcagag tctggcgacg cgggaaatcg catcgtcggt gcagaacgtt
     9661 tcgatccaga ccgatggaac gacccgcgct atgtcgggat tgtccgtggt cgcggatgaa
     9721 accagttccg ccagcaagga ggtgttgcgg gcggccggac aggttcgcga acagacggtt
     9781 acgctgcggc aggaagttgg cagttttctg gccgctaccc gggcggcggg cggcgaccga
     9841 cggccgtgag cgacttgctg atcggagcct ggaggcgaca ctacgccgtg gtgggcagga
     9901 cacgccccgg tggctcccgg tcgtcgatat ctcgcgagga ggcgtcgctt tggagggcga
     9961 ggtgaattat ccagccgcaa ccgatacgac gatcgagatc gccggcgtca accggcctat
    10021 cgccg
//
LOCUS       DASWQN010000008         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_661347, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000008 DASWQN010000000
VERSION     DASWQN010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661347"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2071
                     /locus_tag="VGC82_00135"
     CDS             <1..2071
                     /locus_tag="VGC82_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY0421714.1"
                     /translation="RDSGHLFKDVDLDVSFRSTAPVLRLVDAVFADPAARPGVVPDGE
                     TLTHYADRAGHAGMVEFWPLAPRPDPAPHRPWTVPEQNQSQTSARQLLADTLADWIRA
                     QTGGSVLLESKGRPLTAGDVLILVRRRDEFARALVRALKTRGVPVAGLDRLMLTDQPA
                     VQDLMALADALLLPLDDLTFGCLLTSPLGGFTDDELSELAIDRPGSLWDALRDRASER
                     PSWQRAADFFQVLLARVDYVSPYTLFSEALGPLGGRARLFARLGAEAAEPIDELLNAA
                     LTYAARHPPSLQGFLHWLRRSGAEVKREPEAAGNLVRIMTVHGAKGLQAPLVIVPDTS
                     GLPKDDGSIVWADDSVTGRAVPLWAPRKEFRCDALDRLRAASRQRQMEEHNRLLYVAL
                     TRAEDRLLVCGWSAAHAPKDECWHSLVKRGFEAASAETEPFGPWEGVALRLSAAQAVP
                     AERPDVSARADHAGDLPAWLGAAPGWSPAPPPPEPARPVPLAPSRPEGVDLGPVPATE
                     SPLAEDDAYGNRFRRGQLIHSLLQHLPSVVEPNRRATAMRFLDKPGHGLAANEAEQVA
                     DEVLAVMMHPELAPLFGPGSRAEVPLTGAVGNSVIGGLVDRLVVLPDVVLVADFKTNR
                     RTPVRVEDTPVLYLRQMASYRAVLRAIFPGRAVRCALIWTREAKVTMLPDESLDPHEP
                     GHARDAA"
     gene            2127..2459
                     /gene="trxA"
                     /locus_tag="VGC82_00140"
     CDS             2127..2459
                     /gene="trxA"
                     /locus_tag="VGC82_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007283421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY0421715.1"
                     /translation="MGANTVAVTDQSFSTDVVEKSKSGPVLVDFWAEWCGPCKMIGPA
                     LEELAGEFAGKLTVAKVDIDNNPMSPNQFAVRGIPTLILFKDGKPVSTQVGALPKSKL
                     KDWIAGNI"
BASE COUNT          454 a          909 c         1044 g          545 t
ORIGIN      
        1 ccgtgattcc ggtcatctgt tcaaggatgt ggatctggac gtttcgttcc gctccaccgc
       61 gccagtgttg cggttggtcg atgccgtgtt cgccgacccg gcggcgcgtc cgggcgtggt
      121 cccggacggc gagaccctga cccattacgc cgatcgggcc ggccatgccg ggatggtgga
      181 attttggccg ctggccccgc gccccgatcc ggcaccgcac cggccctgga cggtgccgga
      241 acagaatcag tcgcagacct ccgcccggca gttgctggcc gacacgctgg ccgactggat
      301 ccgcgcccaa accggcgggt cggtgctgct ggagagcaag ggccgcccgt tgaccgcggg
      361 cgatgtcctg atcctggtcc gccgccgcga cgaatttgcc cgagccctgg tgcgggcgct
      421 gaagacgcgc ggcgtgccgg ttgcggggct ggaccggctg atgctgaccg atcagcctgc
      481 ggtgcaggac ctgatggcgc tggcggacgc tctgctgctg cccttggacg atctgacgtt
      541 cggctgcctg ctgaccagcc cgctgggagg tttcaccgat gacgaactgt ccgaactcgc
      601 gatcgaccgc ccggggtcgc tgtgggatgc gctgcgcgac agggcgagcg aacgcccttc
      661 ctggcagcgg gcggcagatt tcttccaggt cttgctggcc cgggttgact acgtttcgcc
      721 gtacacgctg ttctctgaag cgcttggtcc gctgggcggg cgagccaggt tgttcgcccg
      781 cctgggagcg gaggcggcgg aacccatcga cgaattgctg aacgccgcgc tgacctatgc
      841 ggcccggcac ccgccgtcgc tgcaggggtt cctccattgg cttcgccgct caggagcgga
      901 ggtgaaacgg gaacccgaag ccgcgggcaa cctggtccgc atcatgacgg tgcacggtgc
      961 caaaggcttg caggcgccgc tggtcatcgt gccggacacc tcgggattgc cgaaggatga
     1021 cgggtcgatc gtttgggcgg acgacagcgt taccggccgc gccgttccgc tgtgggcgcc
     1081 acgcaaggag ttccgctgcg acgcgctgga ccgcctgcgc gccgccagcc ggcaacggca
     1141 gatggaggag cataaccggc tgctctacgt cgcgctgact cgagccgagg atcgcttgct
     1201 ggtctgcggc tggagtgctg cccatgcgcc caaggatgag tgctggcaca gcctggtgaa
     1261 gcgcggtttc gaggcagctt cggcggaaac ggaaccattc ggcccgtggg agggtgtggc
     1321 gttgcgatta tccgccgctc aggccgtgcc ggccgaaagg cccgacgttt ccgcgcgcgc
     1381 ggaccatgcc ggggatctgc ccgcttggtt aggagctgca cccggttggt cacccgcgcc
     1441 gccaccgccc gagccggccc ggccggtgcc cttggcgcca agtcggccgg agggcgtcga
     1501 tctgggaccg gtgccggcaa cggagtcgcc gctggcggag gatgatgcct acggcaaccg
     1561 gttccgccgc gggcagttga tccattcgtt gctgcagcac ctgccctcgg tcgtggaacc
     1621 aaaccgccgg gcgaccgcga tgcggtttct cgacaagccc ggacatggac tggcggcaaa
     1681 cgaggccgaa caggtcgcgg atgaggtgct ggcggtgatg atgcatcccg aactggcgcc
     1741 attgttcgga cccggcagcc gggcggaggt tccactgacc ggcgcggtgg gaaattcggt
     1801 gatcggcggt ttggtcgatc ggctggtggt gctgcccgac gtggtcttgg tggctgactt
     1861 caagaccaac cggcggacac cggtccgggt ggaggacacg ccggtgctct acctgcggca
     1921 aatggcttcc tacagggctg tgctgagggc gatttttccc ggacgggcgg tgcggtgcgc
     1981 cttgatatgg acgcgggaag ccaaggtgac gatgctgccg gacgagagcc tcgatccgca
     2041 cgaacccggc catgcgcgag acgctgcttg acccggtaaa cggcccgtcc caccttccat
     2101 tcaactgaac ttctgaggat ccgaacatgg gcgcaaatac ggttgcggtg acggaccaga
     2161 gcttctccac ggatgtggtg gagaaaagca aatccggtcc ggtgctggtg gatttctggg
     2221 cggaatggtg cggcccgtgc aagatgatcg ggccagcgct cgaggaactc gccggtgagt
     2281 ttgccggcaa gctgacggtg gcgaaggtcg atatcgacaa caatccgatg tcgccgaacc
     2341 agttcgcagt gcgcggtatc ccgaccttga tcctgttcaa ggacggcaag cccgtgtcca
     2401 cgcaggttgg cgcgctgccg aagagcaagc tgaaggattg gatcgccggg aacatttgaa
     2461 cgcgggctgt ttcagtgccc cgagcggccg gtcgtaagag gcggttcggg gaaagccggt
     2521 gcggcgggcg ttcttagata gggtatgccc ctgttgctgc gtatggtgct tgtcgtcggt
     2581 ggcgacggtt ccgcccgacc gggcgcgcct ttggccgatc gttccgcaga ccctgagttt
     2641 cctcgcctga accgacggtc gggttactgg atctgcgccc gaacatcatt ttgacgcgca
     2701 ccaattttcc atcatgcatg gatatccctg gagcacccca gtggaggcga cagtcggggc
     2761 gcaggttgga gggttctcgc ggccggtggt gcgctgcttc agagcgaaat ccgtgctgag
     2821 agacccactg cctccacttg aggccttgtt ttcatgagca tcttgatccg gtaggtgatc
     2881 cgactggtcg aatgatcctc ttggcgtccg gtggcggctt tgccgttggt tgccgatccg
     2941 ctgcgagtct ca
//
LOCUS       DASWQN010000009         2929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1189273, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000009 DASWQN010000000
VERSION     DASWQN010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189273"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VGC82_00145"
     CDS             <1..187
                     /locus_tag="VGC82_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0421716.1"
                     /translation="AMFVGLLPMAIGISEGSEQNAALARAVLGGISVGTCSTLLFVPF
                     LYALLRRGEAKPLEDYV"
     gene            184..1446
                     /locus_tag="VGC82_00150"
     CDS             184..1446
                     /locus_tag="VGC82_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0421717.1"
                     /translation="MTETPPPPRKRILLFVLVPVVLLLGLGGYLHWRTYADAAETQKQ
                     QQDFVPNVRTATAKKEEAAVKLTLPGQTEAFDTANLFPRATGYVKERRVDIGSRVHAG
                     DLLLRIEAPDLDQQLAQAQAQIGQNQAAVAQAQAQVQSAEANAKLADTTKYRTTTLAS
                     QGWETKQNADNATANQSVQTAGIANARAGIAVAEANLKAQQATVERLLALQAFEQVKA
                     PFAGVITARNVDTGDLLTQDSLGGNPMFSIVRDDVLRIAVYVPQSSAIGIRDGLEAEV
                     TVPEMPNRVFKGRVARSAVALQAASRSMLTEVDVPNPDGVLRAGLFVNVVFAIPREAP
                     AIVVPDEALVFNANGLQVATVDQDGQVRFRKVSVYRDFGTSAELRDGLQGGETLILSP
                     PTELADGSKVRVANPSGNQDTGKQTASR"
     gene            1519..2799
                     /locus_tag="VGC82_00155"
     CDS             1519..2799
                     /locus_tag="VGC82_00155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0421718.1"
                     /translation="MNFRRGWVCLFLFTLTTINYADRVALSVAATPVREEFGLSQVQM
                     GYLLSSFLWTYVFCLIPVGLLVDRFGGKLVNGLGIGLWSAATVLTGFAPNFLFMLGTR
                     MVMGMGESTSWPASNRIIREWFPASERGLANGIFGAGAAAGPAIGAIVISYLVAAFGW
                     RTGFIAAGSVGFIWLLAWVLLFDRPERVGWLGETERQKIIHERDGSRDAGVVEASSLG
                     YLLRQRSVWGLFLTQGTEVYGGYMLLTWLPSYLQQAKGLSIMNAGLLTAVPFALASLF
                     GIALGRISDWVLTPEAVQAGRRRTMIAVMLVGVSTILVVPLIDQLWVIVVILAFVRSL
                     GLAGSALNFALVTDLVRSRADIGKVTAITVVGGNSFGVMAPIVTGYIIALTGSFDRAF
                     IVAGLLPLAGAAATFLMTRSPIEPRGAFGVPNRG"
BASE COUNT          456 a          869 c         1062 g          542 t
ORIGIN      
        1 cgcgatgttc gtgggattgc tgccgatggc gatcggcatc agtgaaggca gcgagcagaa
       61 cgccgccctg gctcgggctg ttctgggcgg gatcagcgtc ggcacctgtt cgacattgct
      121 gttcgtgccg tttctttacg cgctgctgcg acgcggggaa gccaaaccat tggaagatta
      181 cgtatgaccg agacgccgcc gccgccgcgc aagcgcatcc tgctgttcgt gctggtgccg
      241 gtcgtgttgt tgctcgggct gggcggctac ctgcactggc gcacctatgc ggacgccgcg
      301 gaaacccaga agcagcagca ggatttcgtg ccgaacgtgc gcacggcgac ggccaaaaag
      361 gaggaagcgg cggtgaaact gacgcttccg ggccagacgg aggcgtttga cacggccaat
      421 ctgttccccc gggcgaccgg ctatgtgaaa gagcggcggg ttgatatcgg cagccgggtc
      481 catgccggcg acctgctgct gcggatcgag gcccccgatc tggaccagca actcgcacag
      541 gcgcaagcac agatcggtca gaaccaggcc gccgtggcgc aggcgcaggc gcaggtccaa
      601 tcggccgagg ctaacgccaa gctggcggat accacgaaat accgcaccac gaccctggcc
      661 agccagggct gggagaccaa gcagaacgcc gacaatgcga cagccaatca atcggttcag
      721 acagcaggga tcgccaatgc ccgggcgggc atcgcggtgg ccgaggccaa cctgaaggcg
      781 cagcaggcga ccgtcgagcg cctgctggcg ctgcaggcgt tcgagcaggt gaaggcgccg
      841 ttcgcagggg tcatcaccgc gcgcaacgtg gacaccggcg atctgttgac gcaggacagc
      901 ctgggcggca acccgatgtt cagcattgtc cgcgacgatg tcctgcgtat cgccgtctat
      961 gtgccacaaa gcagcgccat cggcatccgc gacggtttgg aagccgaagt caccgtgccg
     1021 gagatgccga accgggtgtt caagggtcgc gtcgcgcgca gtgcggtggc attgcaggcg
     1081 gcatcgcgct ccatgctgac cgaggtcgat gtgccgaacc cggatggcgt gctgcgggcc
     1141 gggctgttcg tcaacgtagt gttcgcgatc ccccgcgagg cccctgccat cgtggttccg
     1201 gatgaggcgc tggtgttcaa cgcaaacggg ctgcaggtgg cgacggtgga ccaggacggc
     1261 caggtgcgct tccgaaaggt ctcggtgtac cgggatttcg gcacctcggc ggagttgcgc
     1321 gatgggctgc aaggtggcga aacgctgatt ctgagtccgc ccacggaatt ggctgacggc
     1381 agcaaggtgc gggtggccaa tccttcgggc aaccaggata ccgggaagca gaccgcgagt
     1441 cgttgacagg cggaatgagg cgcaacaggg tttgggccga cgggcggcgt ccagatcgcc
     1501 ccttggggct ggatcgccat gaattttcgc cgcggctggg tttgcctgtt tctgttcact
     1561 ctgaccacga tcaactacgc cgaccgggtc gcgctgtcgg tcgccgcgac gccggtgcgc
     1621 gaggaattcg gcctgtcgca ggtgcagatg ggctacctgc tgtcgtcctt tctctggacg
     1681 tatgtgttct gcctgatccc ggttggactg ttggtggatc gtttcggcgg caagctggtc
     1741 aacggcttgg gcatcggcct gtggtcggcg gcaacggtgc tgaccgggtt cgcaccgaat
     1801 tttctgttca tgctgggcac ccgcatggtc atgggcatgg gcgaatccac cagttggccg
     1861 gcgtccaacc gcatcatccg cgaatggttt ccggcgtccg agcgtgggct ggcgaacggg
     1921 attttcggcg ccggggcggc cgccggaccg gccatcgggg cgatcgtgat ctcgtatctg
     1981 gtggcggctt ttggctggcg caccggcttc attgccgccg gcagcgtcgg gttcatctgg
     2041 ctgctggcct gggtgctgct gttcgaccgg cccgagcggg tgggctggct gggggaaact
     2101 gaacggcaga agattataca tgagcgcgac ggctcgcggg atgccggcgt ggtggaggcg
     2161 tcctcgcttg gctatctgct gcgccagcgc agcgtctggg gcctgttcct tacgcagggg
     2221 accgaggtct atggcggcta catgctgctg acctggctgc cgtcctatct gcagcaggcc
     2281 aaaggcctca gcatcatgaa cgccggcctg ctgactgccg tgccgttcgc gctggcctcg
     2341 ttgttcggga ttgcgctcgg ccggatcagc gactgggtgc tgacgccgga ggcggtgcag
     2401 gcggggcggc ggcggacaat gatcgcggtg atgctggtgg gggtgtcaac gatcctggtc
     2461 gtgccgctga tcgaccagct ctgggtcatc gtggtgatat tggccttcgt ccgctcgctg
     2521 gggctggccg gcagcgcgct gaacttcgcg ctggtgacgg acctggtgcg cagccgggcg
     2581 gatatcggca aggtgacggc gatcaccgtg gtgggcggca acagctttgg cgtgatggcg
     2641 ccgatcgtga ccggctacat catcgcgctg accggcagct tcgatcgcgc gttcatcgtg
     2701 gcgggcttgc tgccgctggc gggcgcggcg gcgactttcc tgatgacgcg ctcgccgatc
     2761 gagccgcgcg gcgcgttcgg cgtgccgaac cgggggtgag tccggttttt aagccgtggc
     2821 gtgggccgtg cattggctat ccgggcgggc ggtggccata atcatctggt ggcctgccgc
     2881 catgatcatg ttcgtcgtgt ggccgggaat cgtgatccca gtcaccagg
//
LOCUS       DASWQN010000010         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1717209, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000010 DASWQN010000000
VERSION     DASWQN010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717209"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGC82_00160"
     CDS             <1..235
                     /locus_tag="VGC82_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421719.1"
                     /translation="IQANPALARARSALDGLNVFHFPVAFPEVFLRRRPGFDVILGNS
                     PWQEVTLEELVHLAGSGGPVRHGHQRQHLGGRK"
     gene            356..1639
                     /locus_tag="VGC82_00165"
     CDS             356..1639
                     /locus_tag="VGC82_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY0421720.1"
                     /translation="MVSLRFRSEWFKSRSRLGYGADWPLDWREMWHYYGEAEQALKIA
                     GPVTYPWGPKRPRYPYRAHELNGAAELLARGAEALGYQWTATPLATVSAPRGLSPPCV
                     YRGFCRFGCSTNAKQSQLVTYIPRALAAGAEIRDLAMVGRIETGPDGRATGVQYYREN
                     QWRFQKARNVVVAGYAVETPRLLLASANDRYPDGLANSSGLVGKNLMTQSNQAVFGTM
                     ENEVRWNKGPPSLSITEHWNYDDRKNFSGGYCWMSQGPLPIEWVTVQTGSRGLWGEAL
                     RHEMEKYNHQVGFKMVGEMLPDERNRVTLADETDQYGLRVARVTYSWGDNDKALIQHA
                     LGQMEASLEAVGAHDIFRQENDTNHLAGTARMGDDPRTSVVNADCRSWDIPNLWVCDG
                     SVFPTTGGVNPSLTITSIALRTADRIAQLATRGDL"
     gene            1679..>2122
                     /locus_tag="VGC82_00170"
     CDS             1679..>2122
                     /locus_tag="VGC82_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0421721.1"
                     /translation="MARIVVITGAGAGVGRATVAEFARNGCDVALLSRDPDRLESAAE
                     EARRFGVRALPIPTDVADAQAVEAAASRVEAELGPIDVWVNVAMATVFAPVSSLTPEE
                     VARGTAVTYLGQVHGMMAALKRMRGRNRGAIVNVGSALAYRSVPLQ"
BASE COUNT          381 a          642 c          729 g          370 t
ORIGIN      
        1 cattcaggcc aaccccgcac tcgccagggc gcgctcagcc ttggacggcc tcaacgtgtt
       61 tcacttcccg gtcgcgttcc cggaggtctt cctgcgccgg cggccggggt tcgacgtgat
      121 ccttgggaat tcgccgtggc aggaagtgac ccttgaggaa ctagtacacc tcgccggaag
      181 tggcggcccg gttcggcatg gccaccaaag gcaacacctt ggcgggagga aatgatccca
      241 atagagttcg gacctccggc ggggtgtact agggtgatcg acggcgagaa cccgatcatc
      301 atgggggggc aagaacagcg gcaaggcggt aggcggcagt acggtgcatt tcgcgatggt
      361 ttcgctgcga ttccgctccg agtggttcaa gtcacggtcc cggctgggtt acggcgccga
      421 ctggccgctc gattggcggg agatgtggca ctactacggc gaagccgaac aggcgctgaa
      481 gatcgcgggt ccggtgactt atccttgggg cccgaagcgt ccgcgctatc cctatcgcgc
      541 gcatgagttg aatggggcgg ccgaactgct ggcgcggggc gccgaagcgc tgggctacca
      601 atggacggcg actccgcttg cgacggtttc cgccccgcgt ggcctgtcgc cgccctgcgt
      661 gtaccgcggt ttctgccggt ttggctgctc gacaaacgcc aagcagagtc agcttgtcac
      721 ctatattccc cgcgcgctgg cggccggcgc cgaaatacgc gatctggcca tggtcggacg
      781 gatcgagacc ggaccggacg gccgcgccac cggggtgcag tattaccggg aaaaccagtg
      841 gcgcttccag aaggctcgca acgtcgtggt cgccggttat gcagtggaaa ccccgcgcct
      901 gctgctggct tcggcgaatg accgctatcc ggatggtctg gcgaacagtt ccggcctggt
      961 cggcaagaac ctgatgacgc agtccaacca ggccgtcttc ggcaccatgg aaaacgaggt
     1021 tcgctggaat aaaggaccgc cgtcgctcag catcaccgaa cattggaact atgacgaccg
     1081 caagaacttc tccggcgggt actgctggat gtcgcagggg ccgctgccaa tcgagtgggt
     1141 gactgtgcaa accggttcgc ggggcctgtg gggcgaggcg ctgcggcacg agatggagaa
     1201 atacaatcat caggtcggct tcaagatggt cggcgagatg ctgccagacg agcgcaaccg
     1261 cgtgacgctg gctgatgaga ccgaccaata cggtctgcgc gtcgcccgtg tgacctatag
     1321 ctggggagac aatgacaagg cgctgatcca gcatgcgttg ggacagatgg aggcgagcct
     1381 ggaagccgtg ggcgcacacg acatcttccg ccaggagaat gacacgaacc acctcgccgg
     1441 aaccgcgcgc atgggcgatg atccgcgcac cagtgtcgtg aacgccgact gccgcagttg
     1501 ggacattccc aatctctggg tttgcgacgg ttcggtgttt ccgaccaccg gcggggtcaa
     1561 tccttcgctg accataacca gcatcgcgct gcgcaccgcc gatcgcatcg cacagctcgc
     1621 aacgcggggc gacctgtagc ggtagctgcc gcccttttcc aaaccaaggg atcacgaaat
     1681 ggcacgcatt gttgtcatca caggcgccgg tgccggggtc ggccgagcga cggtggccga
     1741 attcgcgcgc aacggctgcg acgtggcact gctgtcgcgg gatccggatc ggctcgaatc
     1801 cgcggcggag gaggcgcggc gatttggggt acgtgcgctg cctattccaa cggacgttgc
     1861 cgacgcgcaa gccgtcgagg cagccgcttc ccgcgtcgag gccgagcttg ggccgatcga
     1921 cgtgtgggtc aacgtcgcga tggcgaccgt gttcgcgccg gtttcgagcc tgacgccgga
     1981 ggaggtcgcg cgaggaaccg ctgtcaccta tctggggcag gttcatggca tgatggcggc
     2041 gctgaagcgg atgcgagggc gcaaccgtgg cgccattgtc aacgtgggct ccgcgcttgc
     2101 gtatcggtcg gttccgctgc ag
//
LOCUS       DASWQN010000011         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1453395, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000011 DASWQN010000000
VERSION     DASWQN010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453395"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            519..782
                     /locus_tag="VGC82_00175"
     CDS             519..782
                     /locus_tag="VGC82_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY0421722.1"
                     /translation="MDLIAVVADGTDLPRTKAGEVVDAVFAAITDALKSKEEVRLVGF
                     GTFAAAIRKASTGRNPRTGEEMAIPESTTVRFKAGKGLKDSVG"
     gene            865..939
                     /locus_tag="VGC82_00180"
     tRNA            865..939
                     /locus_tag="VGC82_00180"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:897..899,aa:Val,seq:tac)
     gene            1080..1156
                     /locus_tag="VGC82_00185"
     tRNA            1080..1156
                     /locus_tag="VGC82_00185"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1114..1116,aa:Asp,seq:gtc)
     gene            1346..1711
                     /locus_tag="VGC82_00190"
     CDS             1346..1711
                     /locus_tag="VGC82_00190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY0421723.1"
                     /translation="MQPVFFGYLPILVFLVIAGGIAIAMVGGSLLAAHQKPYPEKLSA
                     YECGFEAFDDARRRFDVRYYLVAILFIIFDLEVAFLFPWAVALKGIGAFGFWSMIAFL
                     GVLTVGFIYEWCKGALEWE"
     gene            1718..>2094
                     /gene="nuoB"
                     /locus_tag="VGC82_00195"
     CDS             1718..>2094
                     /gene="nuoB"
                     /locus_tag="VGC82_00195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEY0421724.1"
                     /translation="MSDLTMTAWNRDHLPPGPEQDAVIKGITGEIADKGFLVANLDKV
                     VNWARTGSLWPMTFGLACCAVEMIHAYMPRYDLDRLGIIPRPSPRQSDVMIVAGTLTN
                     KMAPALRKVYDQMPEPRWVISMG"
BASE COUNT          360 a          646 c          671 g          417 t
ORIGIN      
        1 cctggcacgc cgtccaatcc cgatcgagtg ggaagagccg gcggacactc cggtgccccc
       61 gccggccgcg cagccagcgg ctgcttcgct gccgcactag ggaaaatcag gccgggcgcc
      121 acagcccccg gcctgatcca cctccgggac gtcgttggag catttcagcg tgcctgcaca
      181 atgttctaac tggccgttgg tcgcatagcc cacgtagatc ggacgttccg tctggcgtga
      241 tcatgccctg gcgacgtggc cggagccagt tctccacatc tcgctgcgac ccgagtaggc
      301 catgacctgc ttcgggctga cggctgttcg gctccccgca ccgggcgggg cttggcctat
      361 tcacagcctg acctgcctcg agacccggtt ctccccagaa atccgccgtc tgagcattga
      421 cgtggccgaa tccggccaag tagtgtccgg ccactctccg agcctgttgc cggagaatcg
      481 agatcttcca caattgagag ggaaacgctg tgaacaaaat ggacctcatt gccgtggtcg
      541 ctgacggtac cgacctcccg cggacgaagg cgggcgaagt cgtcgatgcg gtgttcgccg
      601 ccattaccga cgccctgaaa agcaaggaag aggttcggtt ggtcggcttc ggcacgtttg
      661 cggccgcaat tcgcaaggcc agtacgggcc gcaacccgcg gaccggcgag gaaatggcga
      721 tccccgagtc gaccaccgtt cgcttcaagg cgggcaaggg cctgaaggat tccgtcggct
      781 gatcgggcaa cgggctggca tcgggccttc gcagcaacca gtacagcgag tatgtggcct
      841 gggggccaag aattcgtaag taaagggcgg ttagctcagc ggtagagcgt ctcgtttaca
      901 ccgagagggc cggcggttcg aatccgtcac cgcccaccag gttcccaggg aactcctatg
      961 cggtgccagg ttgccgccgg cccgctttga tcagtcgctt tcgggctgaa agctcgtcga
     1021 tcggtctctt cgcgcttgac gtgatcaatg caccggccta atccctcgcc cctgccgctg
     1081 cgggtgtagc tcagttggtt agagcgccgg cctgtcacgc cggaggtcgc gggttcgagc
     1141 cccgtcactc gcgccagcag cgtgcagttc ttgtgaatgg ttttccagtc agtctggaca
     1201 gttttccaca tttccgcgta tggcggtcgc tgcgggatgc gggtaatgtc cgccgcgtgc
     1261 tgcattgcaa ccggactggc tggcgtcgcc gacgcccgac cgcgtccgaa actcaaggcg
     1321 gcaccggatt tatcgcggac ctgtcatgca gccagttttc tttgggtatc ttcctatcct
     1381 ggtgttcctt gtgatcgccg ggggaatcgc aatcgccatg gtgggtggat cattgcttgc
     1441 cgcccatcag aaaccatatc cggagaagct atcggcctac gagtgtgggt tcgaagcgtt
     1501 cgacgatgcg cggcggcggt tcgacgtacg gtattacctc gtcgcgatcc ttttcatcat
     1561 cttcgacctg gaagtcgctt tcctgttccc gtgggccgtg gccctgaagg ggatcggcgc
     1621 gtttggcttc tggtcgatga tcgcgttcct tggcgttctc accgtgggtt ttatctacga
     1681 gtggtgcaaa ggcgcgctgg agtgggagta gcaacacatg agtgacctga ccatgacggc
     1741 ctggaatcgg gatcatctcc cgccggggcc ggagcaggat gcggtcatca agggcattac
     1801 cggagaaatc gccgacaagg gcttcctcgt cgccaacctg gacaaggtgg tgaactgggc
     1861 gcgcaccggc agcctgtggc caatgacctt cgggctggcc tgctgcgccg tcgagatgat
     1921 ccacgcctac atgccgcgct atgatctgga ccggctgggc atcatcccgc ggcccagtcc
     1981 gcggcagagc gacgtgatga tcgtcgcagg cacgctgacc aacaagatgg ccccggcgtt
     2041 gcgcaaggtg tacgaccaga tgccggagcc gcgctgggtc atcagcatgg ggag
//
LOCUS       DASWQN010000012         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_529709, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000012 DASWQN010000000
VERSION     DASWQN010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529709"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(397..1365)
                     /locus_tag="VGC82_00200"
     CDS             complement(397..1365)
                     /locus_tag="VGC82_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0421725.1"
                     /translation="MKLHPFGSLFPVSTLTLGGGGLGMLWGQTTAEECIATVHAAVAA
                     GINLLDLAPRYGDGKAERVVGDAFGGRLPQGLHITSKCNLGNPAPDRVESVLRQSIET
                     SLRLLQVDKLDLFFLHSNLVPDGHPMWNAADVSRFTSVSLFRSRVRPAFEKLQAEGLI
                     GAWGLTGIGHPDAIIEALAEDPKPAAVQCIANLLDSPGGLKFYDGPAKPRDVMAAARA
                     NGVGVMGIRAVQAGALTAAIDRELPDDHAEVKDFARAAGFRTLCAEIGEDPAIVAHRY
                     ALGLPIDTLVLGVKNRTELAGCVAAAEAGRLPAELVSRIDATVVRS"
     gene            complement(1604..>2439)
                     /locus_tag="VGC82_00205"
     CDS             complement(1604..>2439)
                     /locus_tag="VGC82_00205"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0421726.1"
                     /translation="ALAGHQQDQAMTQLVVRGIGKTYSDGTEALRGVDLHAGDGEIVA
                     ILGGSGCGKTTLLRLIAGLDQASNGNIAVGGQVLTGTHASISAVFQEPRLLPWLNAKA
                     NIAFGGRFLARAERDERADRLLDRIGLTGYGARWPRELSGGQQQRIAIARALIGHPGV
                     LLLDEPFSALDPFTRSSLHDLLLDLWRDMRPTVLMVTHDIEEAVVLGDRIVVMRPHPG
                     RVQETLSVDLPGPRDRLAAAFDLTKRNVLRALNRSLAGAPMGTPASQGQVTGPGVSPW
                     V"
BASE COUNT          465 a          864 c          684 g          426 t
ORIGIN      
        1 cggaacaacc ggtcggaggc gtctttatcc atgagcattg cgagaaggtc acggcttcac
       61 ggcactttct gctcgatcgt cagttgttcc gcgatccggt gaaggatcta cgcgaattca
      121 ccgatgccac ccaactaacg atccgccgct cggggtagat cacgatgatt ccgatctatt
      181 ggactccgct gcttatactt cattcaccac cggagcgcga ccagcgcccg cgttaaaggt
      241 tgagctcgat cgatcgggca gaagccaatg cttcctcctc ggcgctgacg ccccagccgg
      301 acctacccac tcatcgcgca gcctgacctg ccattgcccg atccaaccgt gcgatacgcc
      361 aaaccaaaat tccaccaaga tctgtcagcc atccgatcaa ctacgaacca ccgtcgcgtc
      421 gatccgcgat accaactccg ccggcagcct cccggcttcg gccgccgcca cgcatccggc
      481 caactcggtg cggttcttga cgccaagcac caacgtgtcg atcggcaacc ccagcgcata
      541 acggtgtgcc acaatggccg gatcctcgcc aatctcggca cacagcgttc gaaaacccgc
      601 agctctggca aagtccttca cttccgcatg gtcgtcaggc aattctcgat cgatcgccgc
      661 ggtcagagcg ccggcctgaa cggctcgaat gcccatgacc ccgaccccgt tcgctcgtgc
      721 agccgccatc acgtcgcgcg gcttggccgg cccgtcatag aatttcagcc cgcccggcga
      781 gtccagcagg ttggcgatac actgcaccgc cgccggcttc gggtcctcgg ccagcgcctc
      841 gataatcgca tccggatggc cgattccggt cagcccccag gcgccgatca gcccctcggc
      901 ctgcagtttc tcgaacgccg gccgaacccg cgagcgaaat aaggacaccg aagtaaaccg
      961 actgacatcg gcagcattcc acatcggatg tccgtcaggc accagattgg aatgaaggaa
     1021 aaagaggtcc agcttgtcca cctgcaacag ccgcagactg gtctcgatgg attggcggag
     1081 cacgctctcc acacgatccg gcgccggatt ccccaaattg catttgctgg tgatgtgcag
     1141 gccttgcggc aggcgaccgc cgaacgcgtc accgaccacg cgttccgcct ttccgtcgcc
     1201 atagcgtggc gccaggtcga gcaggttgat gccggctgcg accgccgcat ggacggttgc
     1261 aatgcattcc tcggccgtcg tttggcccca cagcataccc aggccgccgc cgcccagcgt
     1321 cagggtcgag accgggaaca aagaaccgaa tgggtgcagc ttcatcgcat gtccttactg
     1381 ccatttcgcg gatcactcgc aggttgtccg cgcttggtcc agaggctggg ttcgcatacc
     1441 cagagccttt tcagcccgac gatacagggc gaaagtgctc cgcctctttg ttaaccgcat
     1501 gcttcacacg cttcgggcgt tctgcccagc gtcaacgtgc tttagcgaac catcagaacc
     1561 aggggcgcat cagaccccag ggcgcatcag aaccaggggc gcatcacacc cagggcgaca
     1621 cgccgggccc cgtaacctgc ccttggcttg caggcgttcc catcggcgcc cctgccaggg
     1681 accggttcaa cgcccgcagc acattccgct tcgtcaggtc gaacgccgcg gccaatcgat
     1741 cccgcgggcc gggaagatcg acgcttaatg tctcctgcac ccggccggga tggggtcgca
     1801 tcaccacgat acggtccccc agcacgacgg cttcctcgat atcgtgcgtc accatcagga
     1861 ccgtcggccg catgtcccgc cacaaatcca gcagcaggtc gtgcaggctg ctccgggtga
     1921 acggatcgag tgccgagaac ggctcatcca acagcaacac cccgggatga ccgatcagcg
     1981 ccctggcgat ggcgatgcgc tgttgctggc caccggacag ctcacgcggc caccgcgcgc
     2041 cgtatccggt cagcccgatg cggtccagca agcgatctgc gcgctcgtca cgctcggctc
     2101 gggcaagaaa ccgcccgccg aacgcgatgt ttgccttggc attcagccac ggcaacaacc
     2161 gaggctcctg gaaaacggcg ctgatgctgg catgggtccc ggtcagtacc tgccctccga
     2221 ccgcgatgtt tccgttactg gcttggtcca ggccggcgat caggcgcagc aatgtcgtct
     2281 tgccacatcc cgaaccaccc aggatcgcca cgatctcccc atcgccggcg tgcagatcga
     2341 cgccgcgcag agcctcggtg ccgtcgctgt aggtcttgcc gatgccgcgg acgacgagct
     2401 gggtcatagc ctgatcctgt tggtgtcctg ccagcgcag
//
LOCUS       DASWQN010000013         4074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1849432, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000013 DASWQN010000000
VERSION     DASWQN010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849432"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1194)
                     /locus_tag="VGC82_00210"
     CDS             complement(19..1194)
                     /locus_tag="VGC82_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEY0421727.1"
                     /translation="MPIINSIAAFHPEMTAWRHDLHAHPELSMHEVRTSGVVRDKLAE
                     FGVDEIITGMATHGVVGVIRAGSSDRAIGLRADMDALPIHEETGLAYASSSPGVMHAC
                     GHDGHTTMLLGAAKYLAETRNFDGTVYLIFQPAEEFEGGADMMVKDGLFDRCPMDKVY
                     GLHNWPDAKAGTFLWRTGPVMAAVGFFDIVVTGLGSHGAAPHQGIDPIVVSAQIINAL
                     QTIVSRSIEPIEGGVVSVGSIAGGEAYNILPERVAMKGTARWYRPEVGDKIEAGMHRI
                     VKGIAESFGATAELRFFRHAPATVNDAEATALAVDAAQWIAGAEGVREMRAPTMGGED
                     FAFMLNAKQGAYLMLGGARSSNDPLLHHPKYDFNDEILPVGASWWATLVEQQLAKPR"
     gene            complement(1214..2116)
                     /locus_tag="VGC82_00215"
     CDS             complement(1214..2116)
                     /locus_tag="VGC82_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY0421728.1"
                     /translation="MRRGICVSAAMIDPVYVIGASGRSGSALCRSLLADGIPVVPVVR
                     DPARWTALGLAGESAAAARQADLRQPEALRAALAGARRIVSCAHARHTAAIIAASPQA
                     TLVLLGSTRKFTRWPDAHGNGVIAGEAALLGSRQAGVMLHPTMIYGAEGEDNVQRLAG
                     LMRRLPVLPLPGGGRFLVQPIHQSDVTRSIRAALERRWEGPESVVIAGRQAVRYADFA
                     RAVAEAAGLRKPFVIPFPVGPLILGARMTRHVTALPTIQPEEIRRLIEDKAFDIAPMQ
                     AMLGVQPISLTQGLALTFASPHEC"
     gene            2179..3399
                     /locus_tag="VGC82_00220"
     CDS             2179..3399
                     /locus_tag="VGC82_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0421729.1"
                     /translation="MTIQRRAVLGAAAAAAATPILRGRAQSRPVIKIGVMNDQSGPYR
                     DVNGPTGVICTQQAVLEFAAQGFDVEVLTADHQNKPDVAVSLAREWFDQRGVDIIMDI
                     AATSCALALASIVKEKNKIMIATSTASSDVTGKACTPNSMHWVFDTFMEAKSTGGAMV
                     KAGGDTWYFITPNYAFGEATQRDTTKFVEAGGGKVLGSQLYPFPETTDFSAFLVKAQA
                     SGAKILGICGAGADLINIIKQAHEFGLPRTMNLAAMVAYTTDMRSVGIEMAAGTRLSE
                     TYYWDLNDRTRGFQKRIQPKVALWPNMAQAGNYSCTLHYLKTVKDMGAAQAKLSGAET
                     VARMKAMPTDDDCFGPGKIREDGRKIHPAYLFEVKQPSESKHQWDLYKLIGTTPAEDA
                     FRPLSDNACPMVKV"
     gene            complement(3428..>4074)
                     /locus_tag="VGC82_00225"
     CDS             complement(3428..>4074)
                     /locus_tag="VGC82_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEY0421730.1"
                     /translation="AILCRPRVFAGLAVMVVAWPLLPGSLSATTRGVVAWDIGVLVFL
                     ALSAQLFMTETPDHMPAAAEAQQEGEWTIFWLTLAVVIVSFVAVSSEFSAIKGTPSDS
                     KGWQVSLVAVTVFLSWLVTHVTFAFRYAHEFYARDAGGPDVDRGLEFPEEKEPDYLDF
                     FYFALVLGMTFQVSDVQITSRKLRRVATLHGLLSFLFNTVIVAFTVNIAAGLMG"
BASE COUNT          812 a         1392 c         1200 g          670 t
ORIGIN      
        1 ctctcgagga taaggccgtt acctgggctt cgccagttgc tgctccacca gtgtcgccca
       61 ccacgatgcc cccaccggca gaatctcatc gttgaaatca tatttcggat gatgcagcag
      121 cggatcgttc gagcttctgg ctccgcccag catcagatat gcgccctgct tcgcgttcag
      181 catgaaagcg aaatcctcgc cgcccattgt cggtgcccgc atctcgcgca cgccttcggc
      241 cccggcaatc cactgtgccg catcgaccgc cagcgctgtc gcctcggcat cgttcaccgt
      301 cgccggggca tggcggaaaa accgcagctc cgccgtggca ccgaagctct cggcgatgcc
      361 cttcacgatc cgatgcatcc cagcctcgat cttgtcccca acctcaggcc tgtaccatcg
      421 cgccgtgccc ttcatcgcca cccgctcggg caggatattg taagcttcgc cacccgcgat
      481 actgccgaca gagacgacgc cgccctcgat cggctcgatc gaccgggaaa cgatcgtctg
      541 cagcgcattg atgatctgtg ccgagaccac aatcggatcg atgccctgat gcggcgccgc
      601 gccatggctg cccaacccgg tcaccacgat gtcgaaaaat cccactgctg ccatcaccgg
      661 cccggtccgc cacagaaacg tcccggcctt tgcgtccggc cagttgtgca ggccgtacac
      721 cttgtccatc gggcaacggt cgaacaaccc gtccttgacc atcatgtctg cgccgccctc
      781 gaactcttcc gcgggctgga aaatcagata cacggtgcca tcgaagttgc gcgtctccgc
      841 gagatacttc gccgccccca acagcatcgt ggtgtggccg tcatgcccgc aggcatgcat
      901 cacacccgga ctggagctgg catatgccaa cccggtctcc tcgtggatcg gcagcgcatc
      961 catatccgcc cgcaacccga tcgcccggtc cgacgaaccg gcccggatca ccccgacaac
     1021 gccgtgcgtc gccatcccgg tgatgatctc gtccacgccg aattccgcta gcttgtcgcg
     1081 caccacgccc gacgtccgca cctcgtgcat cgacagctcc ggatgagcat gcagatcatg
     1141 ccgccaagca gtcatctcgg ggtgaaaagc ggcgatgctg ttgatgatgg gcataaaagg
     1201 tctccgaaac ggtttaacat tcgtggggcg acgcgaaggt cagtgcaagc ccttgcgtca
     1261 gcgaaatcgg ctggacaccc agcatggcct gcatcggggc gatgtcgaat gccttatcct
     1321 cgatcagccg gcggatttcc tcgggctgga tcgtcggtag tgcagtgacg tgccgggtca
     1381 tcctggcgcc caggatcagc ggccccacgg gaaacggtat cacgaacggc ttgcgcaatc
     1441 cggccgcctc ggccaccgct cgtgcaaagt cggcataccg gacggcctgc cgacccgcga
     1501 tcacgacgct ttccggcccc tcccaccggc gttccagcgc tgcccggatg ctccgcgtca
     1561 cgtcggactg gtgaatcggc tgcaccagga agcggccgcc acccggcaaa ggcagcacgg
     1621 gcagccgccg cataagcccc gccaggcgct gcacattatc ctctccctcg gcgccgtaga
     1681 tcatcgtggg gtgcaacatc acgcccgcct gcctgctgcc caacagcgcc gcctccccgg
     1741 cgatcacccc gttgccatga gcgtccggcc agcgggtgaa cttgcgcgtg ctgcccaaca
     1801 gcaccagcgt tgcctgaggg gaagcagcga tgatcgccgc cgtgtgcctc gcatgggcac
     1861 aggagacgat acgccgagcc ccggcgagtg ccgcccgcag ggcctcgggc tggcgcagat
     1921 cggcctgcct ggccgcggcc gccgattcgc cggccaagcc cagtgccgtc cacctggccg
     1981 ggtctcgcac caccgggacc accggaatgc catcggccag cagggaacgg cacagcgcgg
     2041 atcctgaccg gccggaggca ccaatcacat aaaccggatc gatcatcgcg gctgaaacgc
     2101 atatcccgcg ccgcatgaca aggctctcgc atgaagtgcc agtttcgctt aacctacggc
     2161 aaaattaggg agctccacat gacgatccaa cgacgcgccg ttctgggtgc cgccgcggcg
     2221 gctgccgcga ctccgatttt gcgcggccgc gcccagagca ggcctgtcat caagatcggc
     2281 gtcatgaacg accagtccgg cccataccgc gacgtgaacg gccccaccgg cgtgatctgc
     2341 acgcagcagg cggtgctgga attcgccgcg caaggcttcg acgtggaagt gctgacggcg
     2401 gaccatcaga acaagccgga cgtcgccgtc tcgttggcgc gcgagtggtt cgaccagcgc
     2461 ggcgtggata tcatcatgga catcgccgcg acttcgtgcg cgctggcact ggcgtcgatc
     2521 gtcaaagaga aaaacaagat catgatcgcc accagtacgg catcgtccga cgtgaccggc
     2581 aaggcctgca cgccgaactc gatgcactgg gtgttcgaca ccttcatgga ggcgaaatcc
     2641 accggtggcg ccatggtgaa ggccggcggc gacacctggt acttcattac tccgaactat
     2701 gcgttcggtg aggcgaccca gcgcgacacg acgaagttcg tcgaagccgg cggcggcaag
     2761 gtgcttggtt cgcagcttta tccgtttccc gaaacgactg atttctcggc cttcctggtg
     2821 aaggcgcagg cgtccggagc taagatcctt gggatctgcg gcgccggtgc cgacctgatc
     2881 aacatcatca agcaggcaca tgagttcggg ctgccgcgca ccatgaacct cgcggcgatg
     2941 gtggcctata ccacggacat gcgctccgtc ggaatcgaga tggcagccgg cacgcgcctg
     3001 tcggaaacct attactggga cctgaacgac cgcacccgcg gcttccagaa gcggatacag
     3061 cccaaggtgg cgctgtggcc caatatggcg caggcgggga actattcctg cacgctgcat
     3121 tacctgaaga cggtcaagga tatgggggcc gcacaggcca agctaagcgg cgcggagacg
     3181 gttgcccgga tgaaggccat gccgaccgac gacgattgct tcggtcccgg gaagatccgc
     3241 gaggacggcc gcaagatcca cccggcctat ctgttcgagg tgaagcagcc gtccgaaagc
     3301 aagcaccaat gggatctgta caagctgatc ggcaccacgc cggcggaaga cgcattccgg
     3361 ccattgtcgg acaatgcttg tccgatggtg aaggtatagg cgacgcacgc cgctcggtgg
     3421 tttgccatca acccataagc ccggcggcaa tgttcaccgt gaatgcgacg ataacggtgt
     3481 tgaacaagaa actcagcagc ccgtgcaggg tcgcaacccg acgcaacttg cggctggtga
     3541 tctgcacgtc cgagacctgg aacgtcatcc ccagcaccag cgcaaaatag aagaaatcca
     3601 gataatcggg ctctttttct tcgggaaatt ccagcccgcg atccacgtcg gggccgcccg
     3661 catcacgcgc gtagaattca tgcgcgtagc ggaaggcaaa cgtcacatgc gtcaccagcc
     3721 acgagagaaa cacggtgact gccaccaacg agacctgcca tcccttgcta tccgatggcg
     3781 ttcccttgat cgccgagaat tcgctgctga ccgcgacaaa actgacgatc accacggcca
     3841 gcgtcagcca gaaaatcgtc cactcgcctt cctgctgtgc ctcggccgcg gcgggcatat
     3901 ggtcgggtgt ctcggtcatg aaaagctgcg ccgacagcgc caggaacacg agcacgccga
     3961 tatcccaggc gacgacgccg cgcgtcgttg ccgacaggga accgggcagc agcggccagg
     4021 caacgaccat gacagcgaga ccggcaaaca ccctgggccg gcaaagtatc gccc
//
LOCUS       DASWQN010000014         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2113411, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000014 DASWQN010000000
VERSION     DASWQN010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113411"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VGC82_00230"
     CDS             <1..294
                     /locus_tag="VGC82_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY0421731.1"
                     /translation="EVYTTQVAGYPSVFAHGMLTMGLTGKMLTNYVGDGRLTKFGVRF
                     TNQVWPGDTLQSKATVAAIREEGGQHYVDLTLSTTNQDGKEVVSGSATARLDP"
     gene            complement(417..1487)
                     /gene="ada"
                     /locus_tag="VGC82_00235"
     CDS             complement(417..1487)
                     /gene="ada"
                     /locus_tag="VGC82_00235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEY0421732.1"
                     /translation="MQNTDNIDLAAEEHLWDQVQLRHGGADFLYAVTTMGVYCRPACP
                     SPRPLRKNVRFFRSPTEAEANGFRACRRCDPKGERARIAQDVVRDASAFIEASEDMPS
                     LDALAKRSGYSRFHFLRMFRDHTGLTPRGYAEGVRARRLHVALAGGNRVVDAVAEAGF
                     GSESRVYEKTASLLGMTPGAARRGGEGERIRTAFADCQFGRLLVGATDKGVCFIGFAE
                     PDEALMGDLRRRFPRATISSDDAALGGTVRAVLDFLREPKQALDLPLDLRGTAFQQRV
                     WRTLCGIPAGETRTYAQLAAMAGNPKAIRAVARSCATNPVALAVPCHRVVGSDGGLSG
                     YRWGIPRKKALLEREQTVVEQA"
     gene            complement(1564..>2368)
                     /locus_tag="VGC82_00240"
     CDS             complement(1564..>2368)
                     /locus_tag="VGC82_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0421733.1"
                     /translation="QGLQPTRADGTIRTWGVMANAIFDMDAGFPWMYPYIGIGAGYQW
                     TKFNNISAVGGPFAFSSNTQSGALAGQAIVGASFPVPNMPGLSLTADYRFMDILGGEK
                     YGAAVTTGGPAVPAQLQLHNQFNHDVVVGVRYAFNAPPPPAPPTAPAPVAAPAPAPAR
                     SYLVFFDWDKAGLTERAKSIIKEAADNSSRVQVTRIEVNGYADTSGKAEYNQALSVRR
                     AQAVAGELVRDGVPRNAIDIQGFGDTHLLVSTGPGVREPQNRRVEIVMR"
BASE COUNT          435 a          756 c          780 g          397 t
ORIGIN      
        1 gaggtctaca ccacccaggt cgcaggttac cctagcgtgt tcgcgcacgg gatgctgaca
       61 atgggcctga ccgggaaaat gctgacgaac tacgtcggcg acggcaggct gacaaagttt
      121 ggcgtgcggt tcaccaacca ggtctggccc ggcgacacgc tgcagtcgaa ggctaccgtc
      181 gcggcgatac gagaagaggg cggtcagcat tacgttgatc tgacgctctc gaccaccaat
      241 caggatggca aggaagtggt ctcggggtcg gcgaccgcgc gactcgatcc ctgagaacag
      301 tcaatcccga gaacagccaa tccccgagaa cagccaaccc cggggaacag ccaagcccgg
      361 agggcggtca atccggagag tatgggcggc cggtcggttc cggccgccgt ccgcggtcag
      421 gcctgttcga ccactgtctg ctcccgctcc agcagcgcct tcttgcgggg aattccccag
      481 cggtaccccg atagtccgcc gtcactaccg acgacccggt ggcatggaac tgccagcgca
      541 acgggattgg tcgcacagga ccgcgctacc gccctgatcg ccttgggatt gccggccatc
      601 gccgcaagct gggcgtacgt gcgcgtttca ccggccggaa taccgcacag ggtgcgccag
      661 acccgctgct gaaacgcggt tccccgtaaa tcgagtggta gatccagcgc ctgctttggc
      721 tcgcgaagga aatcgaggac cgcgcggacc gtgccgccga gcgctgcatc atctgaactg
      781 atcgttgcgc gcgggaacct ccggcgcaga tcgcccatca gcgcctcatc cggttcggcg
      841 aagccaatga agcagacgcc cttgtcggtg gcgccgacca gtagccggcc gaactggcag
      901 tcggcgaaag cggtgcggat gcgctcgcct tcgccgcccc ggcgtgccgc gcccggtgtc
      961 atgccgagca aggatgcggt cttctcgtaa acgcggcttt cggagccgaa gccggcctcg
     1021 gcaaccgcat cgacgacccg gttgccaccg gccaatgcca catgcaggcg acgggcacgc
     1081 acgccttcgg cgtacccgcg cggagttagg ccggtatggt cacggaacat ccgcaggaaa
     1141 tggaagcgcg aatagcctga ccgtttcgcc agtgcgtcca aggagggcat gtcctcactg
     1201 gcttcgatga acgcgctggc gtcgcgcacc acgtcctggg cgatcctggc gcgctcaccc
     1261 ttcggatcgc agcgcctgca ggctcggaag ccattggctt cggcttccgt cggagagcgg
     1321 aagaaccgca cgttcttgcg taatggccgg ggcgacgggc aggccggccg gcaatacacg
     1381 cccatggtgg tgacggcata aaggaaatcg gcgccgccat gacggagctg gacctggtcc
     1441 cagagatgct cttccgcagc caggtcgatg ttgtctgtgt tttgcatgtt tgcaatctgg
     1501 tcccggacgg caatctggcg ccatccggct attgcggcga ctcagacagg acggccgaac
     1561 ggatcagcgc atgacgatct cgacccgccg gttctgcggc tcccgcacac ctggacccgt
     1621 ggataccagc agatgggtat cgccaaatcc ctggatgtcg atggcgttcc ggggcacgcc
     1681 atcgcgcacc agttcgcccg cgacggcctg agcgcgccgg accgacaaag cctgattata
     1741 ttcagccttg ccggaggtat cggcgtagcc gttgacctcg atccgggtta cctggacgcg
     1801 gcttgagttg tccgccgcct ccttgatgat ggacttcgcc cgctctgtca ggccggcctt
     1861 gtcccaatcg aagaacacca ggtacgaccg agccggagcg ggcgccggag cggcgacggg
     1921 ggcaggggct gttggcgggg caggcggagg cggcgcattg aaggcgtaac gaacgccgac
     1981 aaccacgtcg tggttgaact ggttatgcaa ttgcaattgg gccggcacag ccgggccccc
     2041 ggtggtcacg gcggcaccgt atttttcgcc gcccaggatg tccatgaacc gatagtcggc
     2101 ggtcagcgac aggcctggca tgttcggcac cgggaaagac gcgccgacga tggcttgtcc
     2161 ggcgagggct ccgctttggg tgttggagct gaacgcgaag ggaccgccaa cagcgctgat
     2221 gttgttgaac ttcgtccact ggtagccggc accgatgccg atatagggat acatccaggg
     2281 aaacccggca tccatgtcga agatggcgtt ggccatcacg ccccaggttc ggatcgtgcc
     2341 atccgcccgg gttggttgca ggccctga
//
LOCUS       DASWQN010000015         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2191, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000015 DASWQN010000000
VERSION     DASWQN010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2191"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VGC82_00245"
     CDS             complement(<1..503)
                     /locus_tag="VGC82_00245"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEY0421734.1"
                     /translation="MGPSQHSIGSAAIQAVRFAAAISRWLGLTLAAVSFFGFTVTANA
                     QGAVKSVHGDWQIRCDTPPGAQAEQCALIQSVVAEDRSNAGLTVIVLKTADQKSKLIR
                     VVAPLGVLLPSGLGLKLDNQDVGRAGFVRCLPIGCVAEVVMDDKLLGQLRNAKTATFI
                     IFETPEEG"
     gene            961..>1509
                     /locus_tag="VGC82_00250"
     CDS             961..>1509
                     /locus_tag="VGC82_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HEY0421735.1"
                     /translation="MKMSKGQIGRRLLGLAVAGVALVAGGTAFAESGQPAPWEYKLQE
                     AASPVMENIVWFHNWLLIIITIITLFVLALLIAVVVKFNARANPIPSRTTHNTLVEVA
                     WTLIPVLILVAIAVPSFRLLFMELDIPKADLTIKATGKQWYWSYAYPDNGKFEFDSLM
                     AQDKQPRLLGVDNEMVVPVNRVI"
BASE COUNT          265 a          443 c          502 g          299 t
ORIGIN      
        1 ccctcttcgg gggtctcgaa gatgatgaag gtcgcggtct tggcgttgcg cagctggccc
       61 aggagcttgt cgtccatcac cacctctgcg acgcagccaa tgggcaggca gcggacaaaa
      121 ccggcgcggc cgacgtcctg attgtcgagt ttcaggccca gtcccgaggg cagcagcacg
      181 ccgagcgggg ccaccacccg gatcagcttg ctcttctgat cggcggtttt cagcacgatg
      241 acggtgaggc cggcattgga gcggtcttcg gccaccacgc tctggatcag cgcgcattgc
      301 tccgcctgcg cgccgggcgg tgtgtcgcag cggatctgcc agtcgccatg gaccgacttg
      361 accgcgccct gcgcgttggc ggtcacggtg aaaccgaaaa acgatacggc ggcaagggtc
      421 agccccagcc agcggctaat cgcggcagcg aatcgcacgg cctggattgc ggcgcttccg
      481 atgctgtgct gcgaaggtcc catcagggtc gatcccttag atatttcctt cggataggtc
      541 ctcaggcggt cgatcgggct gccgaccgga ttattggcaa tggcggcgtc ctgaagcgat
      601 tacagcccgg ctggccgcgc cgcaaccggc gaatcaagtt cctgtggaac ggctgacctg
      661 tgcctacatg gggcaacagg cctgtcaagc gccgcgagcc taggaaagcg cggctttgct
      721 ggatttcgcg cgaaaatgca atctcggcga tccggcggcg atgcctcgct ttggcgatgt
      781 actactgcat tgcgacaggc tgcgaattat ggtttgagag gcgggatttc gacgcgttcg
      841 agcgatctgg ccggggtact tccaagtgct tcccaaggcc gtttgccagg ggcggatcgt
      901 gcagcacttc ctgctatctg gcggagtgct ttggagtttt atcaagggag cgcgggcggc
      961 atgaagatgt cgaagggcca aatcggccgg cgattgctgg ggttagcggt ggctggcgtt
     1021 gcgctggtcg ctggcggaac ggcattcgcc gaatcggggc agccggcgcc gtgggaatac
     1081 aagctgcagg aagcggcctc gccggtgatg gagaacatcg tttggttcca caactggctg
     1141 ctgatcatca tcaccattat cacgctgttc gttctggcgc tgctgatcgc ggtcgtggtg
     1201 aagttcaatg cccgggccaa cccgatcccc tcccggacca cccacaacac gctggtcgag
     1261 gtggcctgga cgttgattcc ggtgctgatt ctggtggcca tcgcggtgcc gtcgttccgg
     1321 ctgttgttca tggagctgga cattcccaag gccgacctca ccatcaaggc gaccggcaag
     1381 cagtggtatt ggagctacgc ctatcccgac aacggcaagt tcgaattcga ttcgctgatg
     1441 gcccaggaca agcagccgcg gctgctcggc gtcgacaacg agatggtggt gccggtcaac
     1501 agggtgatc
//
LOCUS       DASWQN010000016         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1717665, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000016 DASWQN010000000
VERSION     DASWQN010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717665"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            387..1577
                     /locus_tag="VGC82_00255"
     CDS             387..1577
                     /locus_tag="VGC82_00255"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEY0421736.1"
                     /translation="MPWIVALFAVVVLLVVLQDAFEVMLLPRRVHRRVRLTRFYFLIA
                     WAIWCRLASRIQAGVRRERFLSVFGAASMVVLFSLWASALIVAFGSLEWAVQEAAGAS
                     AFREQVYMSGVTFFTLGYGDVVPHSAAARLVTVIEAGTGIGFIAVVIGYLPVLYQLFA
                     RREAHVIQLDARAGSPPTATTMLGRHAAGGGLDKLEHLLRDWEIWCSDLLESHLSYPM
                     LVYYRSQHDNQSWLAALTAVMDCCALILTGVEDLQPLQARMTFAMARQVIVEMARSFR
                     IRPSRYTGGDRLSHADFERMEELLLQSGLHWKGGSQAEETLTGLRATYEPLLDGLATA
                     LLLPLPAWIAAAGAADHWQRGHRGLIASRLIEQLSEGGGESGMQAGGQARFAQRLRSR
                     LRRD"
     gene            complement(1666..>2151)
                     /locus_tag="VGC82_00260"
     CDS             complement(1666..>2151)
                     /locus_tag="VGC82_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY0421737.1"
                     /translation="QLQIWGERTGTPVVAGAQNADSAGLAFDALTKAKATGADVLLID
                     TAGRLHNKAALMDELAKIIRVLRKQDVTAPHSVLLVLDATTGQNAVQQVKVFKEMVAV
                     TGLVVTKLDGSARGGIVVALAETFGLPVHAVGVGEKAGDLRPFDAGEFARGLVGAYSG
                     T"
BASE COUNT          364 a          677 c          665 g          445 t
ORIGIN      
        1 ggggcgggct tgagaaggac gccgtcggct gtcttcgacg gtcagacgtg taccggtgcc
       61 ccatcgccaa cggtcacgaa ccaccttcgg caagatgact tgtcccttgg tagatatcgt
      121 ggtgctgagg cgacccaaca aaggcatatc cgctccatca gcgtaagaca aatgtaagat
      181 gatatgctgg cgccgtgaac gcaagctgtt cgcctggatg caggtcgtcg ccggccggtt
      241 caaacctgat ttcgttcttc ggcaacctga tcgcggtgtc gtcgttcggg tcaatgtcgg
      301 gccgcacgac aacggtgaat gccatttacc gtcaactccg catcggccaa tacgcgatcg
      361 ttcgcggtca ctgaaaggga caaggcttgc cgtggatcgt cgccctgttc gcggtcgtcg
      421 ttctcctcgt cgttctgcag gatgcgttcg aggtcatgct gctgccacgc cgcgttcatc
      481 gccgcgtgcg gctgacgcgc ttctatttcc ttatcgcgtg ggccatctgg tgccggcttg
      541 caagccgcat acaggccggc gtgcgccgcg aacgttttct gagcgtattc ggtgccgcct
      601 cgatggtcgt gttgttcagt ctttgggcca gcgccctgat cgttgctttt ggctcattgg
      661 aatgggctgt gcaggaagcc gccggggcat cggccttccg agagcaggtt tacatgagcg
      721 gcgtgacctt ttttaccctc ggctacggcg atgtcgtgcc gcatagcgcc gccgccaggc
      781 tggtaacggt catcgaggcc ggcaccggca tcggtttcat cgccgtggtg atcggctacc
      841 tgccggtgct gtatcaactg ttcgcccgaa gagaggcgca tgtcattcag ctcgacgccc
      901 gagccggatc gccgccgacg gcaacgacga tgctcggccg gcatgcggcg ggcggcggcc
      961 tggacaagct ggaacacctg ctgcgggatt gggaaatctg gtgctctgac ctgctggaaa
     1021 gccatctgtc gtatccgatg ctggtgtatt accgctcaca acacgacaac cagtcctggc
     1081 tggcggcgct gacggcggtg atggattgtt gcgccttgat cctgaccggc gtggaggatt
     1141 tgcagccgtt gcaggcaagg atgaccttcg ccatggcacg gcaggtgatc gtcgagatgg
     1201 cgcggtcgtt tcgtattcgc ccgtcgcgct acaccggcgg cgaccgcctg tcgcacgccg
     1261 attttgagcg catggaggaa ctacttctgc agtccggcct ccattggaag ggcggttcgc
     1321 aggccgagga aacgctgacg gggctacgtg cgacttatga gccgttgctg gacggcctcg
     1381 ctacggcgct gcttctgccg cttccggcat ggattgccgc ggcgggcgcg gccgaccatt
     1441 ggcaacgtgg ccatcgtggc ttgatcgcca gccggcttat cgaacaatta tcggaaggtg
     1501 gcggcgaaag cgggatgcaa gctggcgggc aagcgcgctt cgcgcaacgg cttcggtccc
     1561 gcttgcgccg ggattgagct acctttttcg atcgcatgat tcacacgctt cggacgttcc
     1621 gcccagcgtc atcatgctct cgtctccgga aaaacagaac aggcactacg tgccggaata
     1681 agccccaacc agtccacgcg cgaactcgcc ggcatcgaac ggacgcaggt cgccagcctt
     1741 ttcacccacg ccaaccgcgt gaaccggcaa gccaaatgtt tccgccaacg cgaccacgat
     1801 gccgccccgg gcgctgccgt ccagcttggt gacgaccaaa ccggtcacgg ccaccatttc
     1861 cttgaacact tttacctgct gcaccgcgtt ctggccggtc gtggcgtcca gtaccagcag
     1921 cacggaatgc ggcgccgtca catcctgttt gcgtaacacc cggatgattt tcgccagctc
     1981 gtccatcagc gcggccttgt tgtgcaaccg cccggcagta tcgatcagca gcacgtccgc
     2041 accagttgct ttcgccttcg tcaacgcgtc gaacgcgagg cccgccgagt ccgcgttctg
     2101 tgcgccggcg acgaccggag tgccggttcg ctcaccccag atctgcaact g
//
LOCUS       DASWQN010000017         7070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_530088, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000017 DASWQN010000000
VERSION     DASWQN010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530088"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VGC82_00265"
     CDS             complement(<1..230)
                     /locus_tag="VGC82_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421738.1"
                     /translation="MPQVFLPIALAISQLNDPTFRGVLLRSLAWSVACLAALYAGSIW
                     TLHHLVALHGWLAWAVDILGSIGASLLAFWLFL"
     gene            379..1953
                     /locus_tag="VGC82_00270"
     CDS             379..1953
                     /locus_tag="VGC82_00270"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY0421739.1"
                     /translation="MTYVPSPHFTQNWVLSKPSAQGRRGIVVSQVRSAAEAGVAILDA
                     GGNAVDAAVATALSLAAVEPWNSGLGGVGFALVHRAGEKLAEVVDFGPAAPRGLNPAA
                     FKLTGRMKQDLFAWPEVEDDTNIHGPLSFAIPASTAGYAKMHASWGRLPLAEVIAPAI
                     ALANRGLPQDWFTTLKIGSSASVLRRYPESARIYLPDGLPPVAPYQGSTGFFRLGALP
                     GTLEQLGRAGLRDQYDGDIAAAIIADVRDMGGVLSAEDLRSCQARILPATQFPWRAHT
                     LQLAGGLTAAPTMVRVLEQIAGLETGPRPDAGWYVAFARAMKQAYKERLEGIGDADPL
                     AAETCTTHLTVCDEDGTMVAMTTTLLSSMGSRVVLPTSGILMNNGVMWFDPRPDQANS
                     IAPGKRPLTNMSPVILRDGDRPWLAAGASGGRRILASVTQMLALITDFGMTPDEAAHY
                     PRVDVSGPDGLSADRRLPPDVIDALAADGPITIVEHGVMPINFACPNIIVSNLEGHRT
                     GISDVASPWSAALAQR"
     gene            2060..4129
                     /locus_tag="VGC82_00275"
     CDS             2060..4129
                     /locus_tag="VGC82_00275"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEY0421740.1"
                     /translation="MFRVASFCVAFSLATSAPVAFASTSFIDGPALPQSQKPVVAYAQ
                     WNELIRKALDGDAQAAVEVGKLYAHGSGAAHDVPEAMRWLSRGVDLDSDEARRELGLL
                     LLKGEGTRKDPEHAAALLHQAADAGDAEAEAALGVMYAYGDGVPQDWALAVDWSLRAA
                     DQDDPWAQANYGLFVESGHAGPADPHQAAVWYRRAASHGFALAQVRLGILYQQGTGVE
                     QDPVHAFDLYLQAARDDNAIAERLLALAYHDGIGTERNVAEGVAWLKRAAQHGDAEAM
                     YLLSDRYAQGQGVVRDPQISARYLLDAAEHGHTLGAARLGVEYVAGSGHDQAARWLQV
                     AIGQAAKEADFLPEDADTYKGRQLTLARYHLGMLLLAGDGVEKDVARAVAMLRLASDR
                     HCAVATDQLAKMYRGGIGVPRDSSMAAKLYEAAAEHGVGSAAASLAEGYMTGWAGERS
                     KAIALRWYRRSAALGFPDAWLALGRFYEESSGVARDNDEALTWYQMAGNNNVAEAQLR
                     LGDIARLGELGQPRNDKVALLWYGLAADQGSSSAEERIGDMYWQGSANLPLDRAKAVY
                     HYGVAAERGVASSARKVALAYANGEGVAPDDAQMLLWERKAAEHGDVVAAGMLGYAIM
                     IGLDGTYDLVEAATWLTLAAENSRPTEWGVHAAVYSRDAQARLTPPEQEAFRARLARL
                     RSSWDGE"
     gene            complement(4136..4492)
                     /locus_tag="VGC82_00280"
     CDS             complement(4136..4492)
                     /locus_tag="VGC82_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0421741.1"
                     /translation="MSLLGKLLGRPTAGPVMWLETDELSAILNGPTPPLVIDVRGAGE
                     FTGPLGHIEAAENIPLDVIPARTEDLLGRQRPLVLVCHTDRRSSAAAEHLRRAGGSDI
                     AVLRGGMVAWRSRPAA"
     gene            4580..5746
                     /locus_tag="VGC82_00285"
     CDS             4580..5746
                     /locus_tag="VGC82_00285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0421742.1"
                     /translation="MDFALTDAQQQIRDEVLRLCARFDDTYWLERENTATFPHAFFDA
                     MAAGQWLGIAMPEAYGGAGLGITEAAILAQAVAESGAAMSGASAIHINIFGLNPVVVY
                     GTEAQKSRMLPPLIEGKDKSCFGVTEPNAGLNTTEITTRADLRGDHYLINGTKIWTST
                     AQVANKILLLARTTPLDRVKRKSQGMTLFYTDLDKTHADVRLIHKMGRHAVDSNMVFF
                     EDMRVPVEDRIGEEGEGFRALLHGLNPERVLIAAEAVGIGRAALAKAARYARERVVFG
                     RPIGQNQGIQHPLAKNWAELEAANLLTFKAAWMYDTGRECGAEANAAKYLAAEAGYRA
                     AEQAVLTHGGMGYAQEYHVERLFRESMLLRIAPVSRELILSYLAERVLDLPKSY"
     gene            5915..6475
                     /locus_tag="VGC82_00290"
     CDS             5915..6475
                     /locus_tag="VGC82_00290"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0421743.1"
                     /translation="MSLVAPQTDGYYSREEFRRWYDAQPNGRFERVDGRIVAMAPERG
                     AHLRVKGAIYRALDRAVTLAGIPCQALPDGATVETGDSDYQPDALVNCGEPMADDAIA
                     APNLVIIVEVLSPGTASTDTGGKLADYFRVPSVVHYLIVHPTRRTITHHRRTDSGIDT
                     RIVVNGSITMAPPGIVITTEEIYGAE"
     gene            6754..>7070
                     /locus_tag="VGC82_00295"
     CDS             6754..>7070
                     /locus_tag="VGC82_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0421744.1"
                     /translation="MPRYIDELNDIGGLSGTDIANIADVSKATVSRWKSGSVKPQPNT
                     QLVLSDLHDFVGWLSEYYAAQQIRIWLIALHPQPDNERAIDLINSNRLVEVPCILDRL
                     DADV"
BASE COUNT         1330 a         2218 c         2249 g         1273 t
ORIGIN      
        1 aggaacaacc aaaacgccag caacgacgcc ccgatcgatc cgaggatatc caccgcccag
       61 gccagccaac cgtgcaaagc cactaggtga tgaagcgtcc agatcgagcc tgcatacagc
      121 gcggccaggc acgccaccga ccaggcaaga cttcgcagca atacgccgcg gaacgtggga
      181 tcgttcaact ggctgatcgc aagggcgatg ggcagaaata cctgtggcat ggttccggtt
      241 ccgcaaatgc tcggccgatc ggccgcgagg aggcccggat cgtcactccg cacggcagaa
      301 cccaagcctg agtggattcc gcgccggagg gaatgtcgcc tatgatctcc cggtgttcaa
      361 ccgccggacg gagatccgat gacctacgtt ccctcgcccc attttaccca gaattgggtc
      421 ctgtccaagc cgtcggccca aggccggcgg ggcattgtcg tgtcgcaggt tcgcagcgcg
      481 gccgaagcgg gggtcgccat cctcgacgcc ggcggcaacg cggtcgatgc agccgtggcg
      541 actgctttgt cgctggcggc ggtcgagccg tggaactccg gcctgggcgg tgtcggcttc
      601 gcccttgtgc accgcgcggg agagaagctg gcggaggttg tcgattttgg tcccgcggca
      661 ccgaggggcc tcaatccagc ggcgttcaag ctgaccgggc gcatgaagca ggacctgttc
      721 gcctggcccg aggtcgaaga cgataccaat attcatggtc cgttgtcgtt tgcgatccct
      781 gcttcgaccg cgggttacgc caagatgcac gcttcctggg ggcggctgcc cctggcggag
      841 gtgatcgccc cggcgatcgc gctcgccaac cgaggactgc cgcaggactg gttcaccacc
      901 ctgaaaatcg gctcatccgc atcggtcctg cgccgctatc ctgaaagcgc ccgcatctac
      961 ctgcccgatg gcttgcctcc ggtcgcaccc tatcagggct ccactggttt cttccgtctc
     1021 ggcgcgctcc ctggcacgct cgagcaactg ggtcgtgccg gactgcgcga ccagtatgac
     1081 ggcgacatcg ccgccgccat catcgccgac gttcgtgaca tggggggcgt actttcagcc
     1141 gaggatcttc ggtcctgcca agcccgcata ctgcccgcga cgcagtttcc ctggcgggcc
     1201 cacacgctgc aactggccgg tgggctgacc gctgctccaa ccatggtccg cgtgctggag
     1261 cagatcgcag ggctggaaac cggaccaagg cccgatgccg gctggtatgt cgcgttcgca
     1321 cgagcaatga agcaggccta caaggagcgg ctggaaggaa ttggcgatgc cgacccgctg
     1381 gccgccgaaa cctgcaccac tcatctgact gtgtgcgacg aggacggcac catggtcgcc
     1441 atgacgacaa ccctgctgtc gtctatggga agccgggtgg tgctgcccac atccggcatc
     1501 ctgatgaaca acggggtcat gtggttcgat ccgcgccccg accaagccaa ctcgatcgca
     1561 cccggcaagc ggccgctgac caacatgtcg ccggttattc tgcgcgacgg tgatcgtccc
     1621 tggctcgcgg ccggagcctc gggcgggcgg cgcatcctcg cttccgtcac gcagatgctg
     1681 gcattgatca cggatttcgg catgaccccc gacgaggcgg cgcattatcc gcgggtcgat
     1741 gtatccggtc ccgacggcct gtccgccgac cggcgcctgc cgcccgacgt gatcgacgcc
     1801 cttgccgcgg acggacccat cacaattgtg gaacacggcg tgatgcccat caacttcgcc
     1861 tgtccgaata ttatagtatc gaatctagaa ggacatcgta ctggaatcag cgacgtagcc
     1921 tctccttggt cggccgctct ggcgcaacgg taacgttatg attttgcaca aacatctgcg
     1981 ggaaccgccg cagaagaatt aacttcactt tttcgtgtgt ttcagatcaa atacacgaaa
     2041 tcgtgaggag ccactctgca tgtttcgtgt cgccagtttc tgtgtcgcat tcagcttggc
     2101 aacttcggct cccgttgctt tcgcctcgac atcgttcatc gacgggccgg ccttgccgca
     2161 atcgcagaaa ccggtcgtcg cctatgcaca gtggaatgaa ctgattcgta aggcactgga
     2221 tggcgatgcg caagcggcgg tcgaagtcgg caagctgtac gcgcacggct caggggcagc
     2281 gcacgatgtg ccggaggcga tgcgctggct tagccgcggc gtcgatctgg attcggacga
     2341 ggcacgccgt gaactggggc ttctgctcct gaagggtgag ggcacccgga aggatcccga
     2401 acatgccgct gcactgcttc accaggctgc cgatgccggc gatgccgagg cggaggccgc
     2461 actcggtgtc atgtacgcgt atggcgacgg agtgccgcag gattgggcgc ttgccgtgga
     2521 ctggtcgctg cgggcggcgg atcaagatga cccatgggca caggcaaact atggcctgtt
     2581 cgtggaatcg ggccatgcgg gtcccgcgga tccccaccaa gccgccgtct ggtaccgcag
     2641 ggcagccagt catggcttcg ccttggcgca agtacggttg gggattctct accaacaagg
     2701 caccggggtc gagcaggatc ccgtccacgc ctttgatctg tatctgcagg ccgcgcgaga
     2761 cgacaacgcg atcgcggaac gccttctggc cttggcctac cacgatggca tcggcaccga
     2821 gcgtaatgtg gccgaaggag tcgcctggct taaacgcgcc gcgcagcacg gtgacgcgga
     2881 ggccatgtac ctgctctccg atcgttacgc ccaagggcaa ggcgtcgtcc gcgacccgca
     2941 gatttcagcg cggtacttgc tcgatgcggc ggagcacggc cataccctgg gtgcggcgcg
     3001 ccttggcgtt gaatacgtcg ccggctccgg tcatgaccag gcggcgcgat ggctccaggt
     3061 tgccatcggc caggccgcga aagaagcgga ttttctgcct gaagatgccg acacctataa
     3121 agggcgtcag ttgacgttag cccgttatca cctcggcatg ctcctgctgg ccggcgacgg
     3181 tgtcgagaaa gatgtggctc gggccgtggc gatgttgcgc cttgcttcgg atcgtcattg
     3241 tgccgtggcc accgatcagc ttgcaaagat gtatcgcggc gggatcggcg ttcctcgcga
     3301 cagcagtatg gcggccaagc tgtacgaagc ggcggcggaa cacggtgtcg gttcggcggc
     3361 ggccagcctc gctgaaggct acatgaccgg atgggctggc gagcggtcca aggcaatcgc
     3421 gctgcgctgg taccggcgat ccgccgccct gggttttccc gatgcctggc ttgctcttgg
     3481 ccgtttctat gaagaatcgt ccggcgtcgc acgggacaac gatgaagcgc tgacctggta
     3541 tcagatggca ggcaacaaca acgtggccga ggcccagctt cgtcttggcg acatcgcccg
     3601 gctcggtgag ttgggccagc cccgcaacga caaggtcgcc ctgctttggt acggcttggc
     3661 cgcggatcaa ggcagcagtt cggccgagga gagaatcggc gacatgtatt ggcagggctc
     3721 cgccaatcta ccgctcgacc gtgctaaagc ggtatatcat tacggcgtgg ccgccgagcg
     3781 tggggttgca tcaagcgcgc gcaaggtggc cctcgcctat gccaacggtg aaggcgtcgc
     3841 cccggatgac gcccagatgc tgctgtggga gcgtaaggcg gcggaacacg gagacgtcgt
     3901 ggcagccggc atgttgggtt acgccatcat gatcggcctc gatgggacct acgacctcgt
     3961 ggaggcggcc acttggctga ccttggcggc cgaaaactcc cgcccgaccg aatggggcgt
     4021 ccacgcggcg gtttactccc gggacgcgca agcgagactg acgccgcctg aacaggaggc
     4081 attccgcgcg cgccttgccc ggttgcggtc ttcctgggac ggcgaatagc gccgatcaag
     4141 cggcgggacg agaccgccac gccaccattc cgccgcgcaa gaccgcgatg tcggatcctc
     4201 cggcgcgccg caaatgctca gcggccgccg aggagcgccg gtcggtgtga cagaccagaa
     4261 ccaggggccg ttgtcggccg agcagatcct cggttcgtgc cgggataacg tccagcggga
     4321 tgttttccgc cgcctcgata tgacccagtg gcccggtaaa ctcaccggcg ccgcggacat
     4381 cgatcaccaa aggcggggtc gggccgttca atatcgctga cagttcatcg gtctccagcc
     4441 acatgaccgg cccagcggtc gggcggccca acagcttgcc cagcaggctc attccagttc
     4501 ctttcctgca gtcgatttcg caccgtcgcg gtggcgcggt agagtggcgt ttggcagcga
     4561 acccggaaag gtcccgatca tggatttcgc actgaccgac gcgcaacagc aaatccgaga
     4621 cgaagtcctg cgtctgtgcg cccggttcga cgacacctac tggctggaac gcgagaacac
     4681 cgcgaccttc ccgcacgcgt tcttcgatgc catggcggcc ggccagtggc taggtatcgc
     4741 gatgcccgag gcttacggag gcgctggcct cggaatcacc gaagccgcca tcctggcgca
     4801 ggccgtcgcc gagtcgggtg ccgcgatgag tggcgcgagc gcgatccata tcaacatctt
     4861 cggcctcaac ccggtcgtcg tttatggcac cgaggcacag aaaagccgga tgctgccgcc
     4921 gttgatcgag ggcaaggaca agtcgtgctt cggggtaacc gaaccgaatg ccggcctcaa
     4981 cacgacagag atcaccacgc gggcagacct gcgcggcgac cattacctga tcaacggcac
     5041 caagatatgg acgtccaccg cacaggtcgc gaacaagatc ctgctgttgg ctcgcaccac
     5101 gccgctcgat cgggtcaagc gcaagtcgca gggcatgacg ctgttctaca ccgacctgga
     5161 caagactcac gccgacgtgc ggttgatcca taagatgggc cggcacgctg tcgattcgaa
     5221 catggtgttt ttcgaggata tgcgtgtgcc ggtcgaggat cgcatcggcg aggaaggcga
     5281 gggattccgc gccctgctgc acggcctgaa tcccgaacgc gttttgatcg ccgccgaagc
     5341 cgtcggtatc ggccgggcgg cgctggccaa ggccgcacgc tatgctcggg agcgggtggt
     5401 ttttggccgg ccgatcggcc agaaccaggg catccagcac ccgctggcga agaactgggc
     5461 cgaactggaa gccgccaacc tgctgacgtt caaggccgcg tggatgtatg acaccggccg
     5521 cgaatgcggg gcggaagcga acgccgcgaa atatctcgcg gccgaggctg gttatcgcgc
     5581 cgccgaacag gctgtgctga cgcatggcgg gatgggctat gcgcaggaat accacgtcga
     5641 gcggctgttc cgcgaaagca tgctgctgcg catcgccccg gtcagccgcg aactgatcct
     5701 gagctatctc gcggaacggg tgctggacct gccaaagtcg tactgaggct tggcaggttg
     5761 cgagggcttc ctgatcacgc cgcggcatat gagcaactgt gggattcgac gcggaacggc
     5821 cgctgctaac ccataccggt ccgtcgccct gagcggtcgc aaatgaccgg caaagcaatt
     5881 cctgatacca tcaggcgcaa gttggagggt tccgatgagc ctcgttgcac cccagacgga
     5941 tgggtactac tcgcgggagg agttcaggcg ctggtacgac gcgcagccga acggccgctt
     6001 tgaacgcgtg gatggccgga ttgtggccat ggcacccgaa cgcggggcgc atctgcgggt
     6061 caagggagca atctatcggg cgctggaccg ggcggtgact ctggccggaa ttccttgcca
     6121 ggcattgccc gatggcgcga cggtcgaaac cggtgacagc gattaccagc cggacgccct
     6181 ggtgaattgt ggagagccaa tggccgacga cgcgatcgcg gcgccaaacc tggtgatcat
     6241 cgtggaagtt ctttcgcccg gcacagcgtc caccgacacc ggcggcaagc tggctgacta
     6301 ttttcgggtc ccatccgtgg tgcattacct gatcgtccat ccaacccgcc gcacgattac
     6361 ccatcaccgc agaaccgatt ccggcattga cacgcggatc gttgtcaacg gatcaatcac
     6421 gatggctccc cccgggatcg tcatcaccac tgaagaaatc tacggcgctg aataggcttg
     6481 gcgtcggtac taccaggcgc ggaggcggat tttgtggacg gctgggtcgc tgctttcgat
     6541 ctgaagccaa gtttcgcggc tacctgaagg ctcggggtca tggagtactt gacgcatagt
     6601 tgtttcttgg aggacgcttc ctgaaacaac tacgcgccgc tcgatgatga atgagcaaat
     6661 cctgatgatg actgatcaag ccctgccatt ttcaaaggcg gcttttaata caagatccag
     6721 tcagaattca ccattagcac caagtcaggg gcgatgcccc gctacattga cgagttaaac
     6781 gacataggcg gcctcagtgg aaccgacatc gccaacatcg cggacgtgtc gaaagcgact
     6841 gtctcacgat ggaagtccgg ttcggtcaaa ccgcagccga acacccagct ggttctctcc
     6901 gacctccacg actttgttgg ttggctcagc gagtattatg cggcgcaaca aatccgaatc
     6961 tggctcatcg ccctccatcc ccagcctgac aacgaacgcg ccatcgacct catcaattcg
     7021 aaccgcttgg ttgaagtgcc ttgcatcctt gaccgcctcg acgcggacgt
//
LOCUS       DASWQN010000018         4945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_134390, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000018 DASWQN010000000
VERSION     DASWQN010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134390"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VGC82_00300"
     CDS             complement(<1..267)
                     /locus_tag="VGC82_00300"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0421745.1"
                     /translation="MFIAMNRFKVLPGAESAFEEVWTSRDTHLPGVPGFVSFHLLRGP
                     VREDHVLYSSHTIWRDKAAFEGWTTSEAFRQAHRGAGGNKPLYLG"
     gene            complement(329..991)
                     /locus_tag="VGC82_00305"
     CDS             complement(329..991)
                     /locus_tag="VGC82_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0421746.1"
                     /translation="MDDVVFLFDVDNTLLDNDQVQSDLSDHLANDYGADARDRYWTLF
                     EDLRGTLGYADYLGALQRYRLEDLHNPKVLRIANWMADYPFAERLYPRALDVVGHIGA
                     WGKTVILSDGDAAFQPRKVERSGLWRAFADNVLIYVHKEQALDDVERLYPARHYVMID
                     DKLRILEALKQQWGDRVTTVFPRQGHYAFDPEELRKRRPADIAIDAIGDLMGFGADRF
                     TA"
     gene            complement(1000..1980)
                     /gene="lpxK"
                     /locus_tag="VGC82_00310"
     CDS             complement(1000..1980)
                     /gene="lpxK"
                     /locus_tag="VGC82_00310"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY0421747.1"
                     /translation="MPVAPAFWSHDGTLARALAPFSVLGAVLTARRVARPGWRAPVPV
                     VCCGNVTVGGAGKTTLVLDLAHRLAGRGVHILLRGYGGSSRGVHQVAPDDPASLVGDE
                     ALLLVQAAPTWIGADRAASAKAAIEAGAKILLLDDGLQNPTLEKTASILVIDGRSGFG
                     NGRVLPAGPLREPVADAASRCQAAVLIGPDLAQVSTCLPRWLPVLRADLVQDPSIAAL
                     AGRLVVAFAGIASPEKFFAPLRQAGAILAVAQAFPDHHVYTARELDALVRDARDRQAI
                     LVTTPKDAVRLPPAIRSQVTIVGVSLAWREPAQIDQFLDNAVAGTPFIVR"
     gene            complement(1983..3260)
                     /locus_tag="VGC82_00315"
     CDS             complement(1983..3260)
                     /locus_tag="VGC82_00315"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEY0421748.1"
                     /translation="MSIVTGDALSTGWAAAAALLAPALRLHLRRRAANGREIAGRLPE
                     RRGIDATPRPAGPLLWMHAASVGETLSILPVLEVLRHRAKILLTTGTVTSQTLLDRRI
                     PEHGLAGDVLHRFAPLDVPAWIGRFLSHWRPDAACFVESELWPNQIAACRTRGIPLML
                     LNARMSERSFKRWRYAPRFARHLLGGFVQIQARTDEDAERYRTLGASHVDSPGDLKFA
                     TPPLPVDRTEFDRLKRLLGDRPVWLAASTHPGEEACIAATHRLVATTHPELLTIIAPR
                     HAVRGAEIAAELGAPRRSAGQDPPEGGIWIADTMGELGLWYRLARIAFVGRSLIAPGG
                     GQNPLEPARLGCAIAVGPYIGNFTDHVALLRDAGGLVDVADGAALARFVSAMLDNPDQ
                     RRRLGDHAAACVTRHSDLPARMAEALASLLPAA"
     gene            complement(3248..3922)
                     /locus_tag="VGC82_00320"
     CDS             complement(3248..3922)
                     /locus_tag="VGC82_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0421749.1"
                     /translation="MKFLLRTRPAQAILAFLLGSYLRFALRTTRWTLDGQENFAPHGA
                     GVPAVFAIWHEFLPLMPALSLIARTLPFYRPTPIRSLVSQHRDGRFIGAVVRRFGIIP
                     IMGSSTRGGAAGLRNLLAALRRGHLIGITPDGPRGPRRQAAFGVAQVAALSGVPVVPL
                     AARTTWRIQLNTWDRMPVPLPFGRGVMVCGRAIDVPRHDWKNAVPLIADALNKAAARA
                     DELCPS"
     gene            complement(3999..>4945)
                     /locus_tag="VGC82_00325"
     CDS             complement(3999..>4945)
                     /locus_tag="VGC82_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0421750.1"
                     /translation="FHGSKGLFSNRSILNQAVHNIIPLLRSTLGANVGISIKASPVLS
                     PALADLTQLELAILNLAINARDAMPDGGSITIETADVALGEPVMPEEPASGDYVAVSV
                     SDTGTGIPDAVREKIFEPFFTTKDVGKGSGLGLSQVLGVVKQLGGGLTVRSAPAEGTR
                     ISIFLPRADEASITVTKGPYGEISREASIRPDASQRCCLLLVDDDTDVRSIAADMLLE
                     SGYDVVEASSGAAALKVLERADNRVELILADVVMPGINGVELASIVRRDWPALPVLLM
                     TGYADSGLLRQSGKMDVLRKPFGSAELEEKLQQILARK"
BASE COUNT          971 a         1661 c         1524 g          789 t
ORIGIN      
        1 tcccaggtac agcggcttgt tgccgcccgc gccacggtgc gcctggcgaa atgcctcgga
       61 tgtcgtccaa ccctcgaatg ctgccttgtc tcgccagatg gtgtgcgagg aatacaacac
      121 atgatcctcc cgcaccggcc cgcgcaacaa atggaacgac acaaagcccg gtacaccggg
      181 caaatgggtg tcgcgtgaag tccagacttc ctcgaacgcc gactctgcgc ccggcagaac
      241 cttgaagcga ttcatcgcga tgaacatggg cgtctccttt ggacctgctg atagtctggt
      301 gatccacgcc tgcgatcgcc acccatgatc acgcggtaaa ccggtccgct ccgaagccca
      361 tcaggtcacc gatcgcatcg atggcgatat ccgccggccg ccgcttgcgc agttcctccg
      421 gatcgaaggc atagtgcccc tggcgcggga agaccgtcgt cacccggtcg ccccattgct
      481 gcttcagcgc ttccaggatg cgcagcttgt cgtcgatcat gacataatgc cgagccggat
      541 acagccgctc gacatcgtcc aaagcctgct ccttgtggac gtagatcagc acgttgtcgg
      601 cgaacgcccg ccacagtccg gaacgttcga ccttacgcgg ctgaaaggct gcgtcgccat
      661 ccgaaagaat aaccgtcttg ccccaggcgc cgatgtggcc aacgacatcc agcgcccgag
      721 gatacagtcg ttcggcgaac gggtagtccg ccatccagtt ggcgatacgc agcactttcg
      781 gattatgcag atcctccagc cggtagcgct ggagcgcgcc gaggtaatcg gcatacccca
      841 aggttcctcg cagatcctcg aacagggtcc aatagcgatc gcgggcgtca gcgccgtaat
      901 cgttggcgag gtgatcgctc aggtcgctct ggacctgatc gttgtccagc agcgtgttgt
      961 cgacatcgaa cagaaaaacc acatcgtcca tggcgggcat taacgcacga taaagggggt
     1021 gcctgcgacc gcgttgtcga gaaattgatc gatctgcgcc ggttcccgcc acgccaggct
     1081 gacgccgacg atcgtcactt gggaccggat ggccggcggc aggcggaccg cgtctttcgg
     1141 ggtggtgacc aggatcgcct gccggtcacg cgcatcgcgc accagtgcgt caagttcgcg
     1201 cgcggtatag acgtgatgat ccgggaacgc ctgggcgacc gcaagtatgg cgcccgcctg
     1261 gcgcaacgga gcgaagaatt tctccggcga ggcgatcccg gcgaacgcga cgaccaggcg
     1321 gcccgcgagc gcggcgatcg agggatcctg caccagatcc gcccgcagca ccggcaacca
     1381 acgcggcagg caagtcgaaa cctgagccag atccggaccg atcaataccg ccgcctgaca
     1441 gcgggaggca gcgtcagcca cgggttcccg cagtggaccg gccggaagca cgcggccatt
     1501 gccgaacccg gagcggccgt cgattaccag gatcgaggca gtcttttcca acgttggatt
     1561 ttgcaggccg tcatccagca gcagaatttt cgccccggcc tcgatcgcgg ctttcgcact
     1621 tgcagcccga tcggcaccaa tccaggttgg tgcggcctgc acaagcaaca gcgcctcatc
     1681 cccaaccagc gacgccggat catccggcgc cacctgatgc accccgcgtg aggagccgcc
     1741 ataacctcgc agcagaatgt gcacgcctcg gccggccagc cggtgcgcga ggtccagcac
     1801 caaagtggtc ttcccggccc ctccgacggt aacgttgcca cagcagacca ccggcaccgg
     1861 cgcacgccag cctggccgcg ccacccgccg cgccgtcaaa accgccccca gcaccgagaa
     1921 cggagccagc gctcgggcca aagttccatc atgtgaccag aaggcaggtg ccaccggcat
     1981 cgtcaggctg ctggcagcag ggacgccagc gcctcggcca tacgagcggg caggtccgaa
     2041 tggcgtgtaa cgcaggcggc cgcatgatcg cccagacgcc gccgctggtc aggattatcg
     2101 agcatcgccg acacgaaccg cgccagggcg gcaccatccg ccacatccac aagcccgccg
     2161 gcatcgcgca acagcgccac gtggtcggta aagttgccga tatacggccc gaccgcgatc
     2221 gcacatccca ggcgcgccgg ctcaagtgga ttctgtccac cgcccggggc gatcaggctc
     2281 cggccgacga acgcgatccg tgcaagccgg taccataatc ccagttcacc catcgtgtcg
     2341 gcgatccata tcccgccttc aggcggatcc tgccccgccg accgccgcgg agcaccaagt
     2401 tctgccgcga tttcggcgcc tcgcacggcg tgtcggggcg cgatgatcgt caacagttct
     2461 ggatgcgtcg tcgcgaccaa tcggtgggtg gcggcaatac acgcttcctc cccgggatgg
     2521 gtgctcgcgg cgagccatac cgggcgatcg cccagaagcc gtttcaaacg atcgaattcc
     2581 gtccgatcca ccggcagcgg cggggtggcg aatttcaggt ctcctggaga gtcgacatgg
     2641 cttgcaccga gcgttctgta gcgttcggca tcctcgtcgg tgcgagcctg gatttggacg
     2701 aagccgccca gcaaatggcg ggcaaaccgc ggggcgtatc gccaacgctt gaagctgcgc
     2761 tcggacatcc gcgcattcag caacatcaac gggatgcctc tggttcggca cgccgcgatc
     2821 tggttgggcc aaagctcgct ttcgacgaaa caagcggcgt cgggccgcca atgcgacaga
     2881 aaccggccga tccatgcggg gacgtccagc ggcgcaaacc ggtgcagcac gtctccggcc
     2941 aaaccgtgct ccggaatgcg ccggtcgagc agcgtctggg acgtcaccgt gccagtcgtc
     3001 agcagaatct tcgcgcgatg gcgcagcacc tccagaaccg gcaggatcga gagcgtttct
     3061 cccacgctgg cggcatgcat ccacagcaaa ggtccggccg gccgaggagt ggcatcgatg
     3121 ccgcggcgtt cgggcaaccg gccggcgatt tccctcccgt ttgcggcgcg gcggcgcagg
     3181 tgcaggcgaa gcgccggagc cagcagggcc gccgcggccg cccagccggt ggacagagca
     3241 tcgccggtca cgatggacac aactcatccg cccgggcggc agccttgttc agggcatcgg
     3301 cgatcagggg gacggcgttt ttccaatcgt gccggggcac atcgatcgcc cggccgcata
     3361 ccatcacccc tcggccgaac ggcagcggca cgggcatccg gtcccaggta ttgagctgaa
     3421 tccgccacgt cgtgcgcgcc gcgagcggca ccaccggcac gcccgaaagt gccgcaacct
     3481 gggccacgcc gaaagcggcc tgacggcgcg ggccgcgcgg gccatccggc gttataccaa
     3541 tcagatggcc ccgccgaagg gccgccagca ggttgcgcaa gccggcagcc ccaccgcgcg
     3601 tggaggatcc cataatcgga atgatgccaa agcgccgcac caccgcgccg atgaaccgtc
     3661 catcgcgatg ctggcttacc aggctgcgga tcggtgtcgg gcggtagaac ggcagcgtgc
     3721 gcgcgatcag cgacaaggcc ggcatcagcg gcagaaattc atgccaaata gcgaagaccg
     3781 cggggactcc ggcgccatgc ggggcaaaat tttcctgccc atccaatgtc cagcgcgtgg
     3841 tacggagagc gaacctcagg tagctgccca gcaaaaacgc caggatggct tgggcgggcc
     3901 gtgtgcgcag caggaacttc atcggcgggc cggtagcacg gggaaccgat gttacggaag
     3961 tggcgaaaag cgtcctgtcc caacggaccc cacgcggatc atttccgtgc caggatctgc
     4021 tgcagttttt cttccagttc agcggacccg aatggcttcc tgagcacgtc cattttgccg
     4081 ctttggcgca acaggccact atccgcatac ccggtcatca ggagaaccgg caaagcgggc
     4141 caatctcgcc ggacaatcga cgcaagctcc acgccgttga tccccggcat cacaacgtct
     4201 gccaggatca gttcgactcg gttatcggcc cgctccaaca ccttgagcgc tgcggcgccc
     4261 gatgacgctt cgaccacgtc gtaacccgat tcaagcaaca tgtcggcggc gatcgatcgc
     4321 acatcggtat cgtcgtccac gagaagaaga cagcagcgtt gcgatgcatc ggggcggatg
     4381 gaggcttcgc gcgaaatctc gccgtacggc ccctttgtca cggttatcga agcctcgtca
     4441 gcccgcggta ggaagatgga gatgcgggtc ccttcggcag gtgcggatcg gacggttaag
     4501 cctccgccaa gctgtttcac cacgcccaga acctgagaga gtccaaggcc agatcccttg
     4561 ccaacgtctt tcgtggtgaa gaaaggctcg aaaatcttct cgcgcaccgc gtccggtatc
     4621 ccggttccgg tgtcggatac gctcacggca acatagtccc ctgaagccgg ctcctcgggc
     4681 atgacgggtt cgccaagagc gacatcagcg gtttcgatgg tgatcgaacc gccatccggc
     4741 atcgcatccc gcgcattgat cgccaggttc aggatcgcca gctccaactg cgtcagatcg
     4801 gcgagcgcgg gcgacaggac aggagacgcc ttgatgctga tcccgacatt ggcaccgagc
     4861 gtgctccgca gcagcggtat tatgttatga accgcttggt tcaaaatcga ccggttcgag
     4921 aacaatcctt tgctgccgtg aaaac
//
LOCUS       DASWQN010000019         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_266420, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000019 DASWQN010000000
VERSION     DASWQN010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266420"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1236
                     /gene="rutA"
                     /locus_tag="VGC82_00330"
     CDS             157..1236
                     /gene="rutA"
                     /locus_tag="VGC82_00330"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HEY0421751.1"
                     /translation="MQIGVFIPIGNNGWLISENSPQYWPSFALNKQVTLTAEKHGLDF
                     VLSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTTRIKLFATCATLVIPPAIAARMAS
                     TIDSISDGRFGLNLITGWQKPEYSQMGLWPGDEYFGRRYQYVSEYIQIMRQLWETGHS
                     DFKGEFFKMEDCRLSPRPQVPMKIICAGQSEAGMAFSAQYADYNFCFGKGVNTPVAFA
                     PTVAKLMAANARTGRDVRAYALFMIIADETDEAARAKWEHYKAGTDLAAVAWLVEQSD
                     ADKSSGGDTNVRQMVDPVSVVNINMGTLVGSYETVARLLDEVDTVDGCDGVLLTFDDF
                     IKGTEDFGARIQPLMKSRHTAKAAA"
     gene            1233..>1648
                     /locus_tag="VGC82_00335"
     CDS             1233..>1648
                     /locus_tag="VGC82_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY0421752.1"
                     /translation="MNASVATGYTAPDARTESITLPARPEPLRLKPSETAVIVVDMQN
                     AYASVGGYIDIAGFDVSGAPPAIAKTRTVLDAARAAGVTVIFFQNGWDKEYQEAGTTG
                     SPNWHKSNALKTMRARPELQGQLLARGGWDYAIVDAL"
BASE COUNT          334 a          528 c          491 g          295 t
ORIGIN      
        1 aaccgctgtc gtagggcggc gatttcagcg cgcaccatga caccatcatc aaccgctgtc
       61 gtgggtcggg caagccgagc gccggccatg acaccatcat caaccgctgt caggggcggc
      121 tgtgcctgaa gcggttattt cggagtaggt ctaagcatgc aaattggcgt ctttatcccc
      181 atcggcaaca atggctggct gatttcggag aactcgccgc agtactggcc cagtttcgcg
      241 ctgaacaagc aggtcacgct cacggctgaa aaacatggcc tggatttcgt cctgtcgatg
      301 atcaaactgc gcggctttgg cggcaagacg gagttctggg accacaacct cgaatccttc
      361 accctgatgg ccggccttgc ggccgtcacc actcgcatca agctgttcgc cacctgcgca
      421 acgctggtga tcccgccggc gatcgccgcg cgcatggcct ccacgatcga ctccatctcg
      481 gacgggcgct tcggcctcaa tctgatcacc ggctggcaga agccggaata ttcccagatg
      541 ggtttatggc cgggcgacga atatttcggc cgccggtacc agtacgtctc ggaatacatc
      601 cagatcatgc ggcaattgtg ggaaaccggc cactccgact tcaaaggcga attcttcaag
      661 atggaggatt gccggctgag cccccgcccg caggttccga tgaaaatcat ctgcgccggc
      721 caaagcgaag ccggaatggc attctccgcc cagtatgccg actacaattt ctgcttcggc
      781 aagggtgtca atacacctgt cgcgttcgcg cccacggtcg ccaagctgat ggcggcgaac
      841 gccaggaccg gacgggacgt gcgcgcctat gccctgttca tgatcatcgc cgatgaaacc
      901 gacgaagccg cgcgggccaa atgggagcat tacaaggccg gcaccgatct tgccgccgtc
      961 gcctggctgg tcgaacaatc ggacgccgac aaatcctccg gcggcgacac caacgtgcgg
     1021 cagatggtcg atccggtttc cgtcgtgaac atcaacatgg ggacgcttgt ggggtcatac
     1081 gaaaccgtcg cccgcctgtt ggatgaggtc gataccgtgg atggctgcga tggcgtgctc
     1141 ctgactttcg atgacttcat caagggcacc gaggatttcg gcgcccgcat ccagccgctg
     1201 atgaaatccc gccacaccgc aaaggccgcc gcatgaacgc atccgttgcc accggctaca
     1261 ccgcgcccga cgcacgaacc gagagcataa ccctgccggc tcgccccgaa ccgctgcggc
     1321 ttaaaccgag cgaaacggcg gtgatcgtgg tcgatatgca gaacgcttat gcgtccgtcg
     1381 ggggctatat cgacatcgcc ggcttcgacg tgtccggcgc gccgccggcg atcgccaaga
     1441 ccaggaccgt gctcgatgcg gcacgcgccg ccggagtgac ggtgattttc ttccagaacg
     1501 gctgggacaa ggagtaccag gaggccggca ccaccggctc ccccaactgg cacaagtcca
     1561 acgccctgaa gacgatgcgg gcacgccccg agcttcaggg gcaattgctg gcacgcggcg
     1621 gctgggatta tgcgatcgtc gatgcact
//
LOCUS       DASWQN010000020         1829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1190103, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000020 DASWQN010000000
VERSION     DASWQN010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190103"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..1663)
                     /locus_tag="VGC82_00340"
     CDS             complement(266..1663)
                     /locus_tag="VGC82_00340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0421753.1"
                     /translation="MEIQTHFRRWWILAVVVGGQFMFVVDAFIVNVAIPSIRADLQAS
                     GGAMEAVIAIYQIGFATLVIVGGRLGDMFGRKRLFLIGLLGFTAASIDCGLANSAFML
                     IVARLGQGAAAALMSPQVLATIHTLFPDAARSRAFAIFGITLGLGGATGFLLGGWLIQ
                     LDIGGLGWRTVFFVNGPVGVALAAAAVVLMPADTGRRAGGLDLRGAAFLFVGLLSLIG
                     PVLAARDLNYAWWLWILEALGAAILVAFLGLERRTEQAGGTTLIDPALLEDRIFLLGI
                     AATACFFAANMSVYLVVTLFLQNSLGWSAFEAGCSVLPLALAFVVASRHGGRRAATRG
                     LRALIEGCLLEAAGLVVVALVVAGGAASLPMILALGVFGYGQGLVMAPLFGAVLTTVK
                     HAHAGSGAGMLTTVQQAANAAGVAVIGGLYFAVQPMVGDTGALLASLLDAVIALLATT
                     ALLRCMGRVALRVAA"
BASE COUNT          394 a          607 c          568 g          260 t
ORIGIN      
        1 agcatgaagc agggttttag cgggacgttc gcaatgctcg acgaatactt ggctaagctg
       61 caaggtggag cggcctaacg ggttgatcct gccccgcaac gccgcggtcg cttcacacca
      121 tatccgttgg acggatgccg ctatgcgttc tagccgcgtg tgctttggcc gccacccacg
      181 atttgccttc gaggcaaagt tttgggtggc ggcgttaaga acggccaaga cacgatattc
      241 aacctgactc cggcgggcgt tcgcctcagg ccgcgacccg gagggccacc cggcccatgc
      301 accgcaagag cgccgttgtg gctaacaggg caatcacggc gtcgagcagc gatgccagca
      361 aagcaccggt gtctcccacc atcggctgaa ccgcgaagta caagccgccg atgaccgcca
      421 cgccggcggc gttggccgcc tgctgtaccg tggtcagcat cccggcgccc gacccggcat
      481 gggcgtgctt cacggtggtt aacaccgcac cgaacagcgg tgccatcacc agcccctgcc
      541 cgtagccgaa cacgccgagt gcgaggatca tcggcaacga agcggcaccg ccggccacca
      601 ccagggcgac aaccaccagt cccgcggcct cgagcaggca gccttcgatc aatgcacgaa
      661 gacctcgggt ggccgcgcgc cgaccgccgt ggcgggatgc gacgacgaat gccagggcca
      721 gcggcaggac gctgcatccg gcctcgaacg ccgaccagcc caggctattt tgcaagaaca
      781 gcgtcaccac cagatagacc gacatgttgg cggcgaagaa gcaggcggtg gcggcgattc
      841 ccagcagaaa aattctgtcc tccagcaggg ctggatcgat cagcgtcgtg ccgccggctt
      901 gttcggtacg gcgttccaac ccgaggaatg cgaccaggat cgcggctccg agcgcctcga
      961 ggatccagag ccaccaggca tagttcaggt cgcgcgcagc cagcaccggt ccgatcaggg
     1021 acaacaggcc gacaaacaga aacgcggcgc cgcgcaaatc cagcccaccg gcgcggcggc
     1081 ctgtatcggc cggcatcagc acgaccgccg ccgccgccag ggcaacgccc accggtccgt
     1141 tgacgaaaaa cacggtcctc cagcccaggc cgccgatatc cagttggatc agccagccgc
     1201 ccagcagaaa accggtggca ccaccgagac ccagcgtgat gccaaatatc gcgaaggcgc
     1261 gggatcgggc ggcatccggg aacagggtat ggatggtcgc caggacttgg ggcgacatca
     1321 atgccgccgc cgcaccctgg cccagtcgcg cgacaatcag cataaaggcg gagttggcga
     1381 ggccgcagtc gatcgacgcc gccgtgaagc ccagcaggcc gatcaggaac agccgcttgc
     1441 ggccgaacat gtcgcccaac cggccgccaa cgatgaccag ggtggcgaag ccgatctggt
     1501 agatcgcgat gactgcctcc atcgccccgc ccgaggcctg aaggtcagcc cggatggacg
     1561 ggatggcgac gttcacgatg aacgcgtcga cgacgaacat gaactggccg ccgaccacga
     1621 cggccaggat ccaccagcga cgaaagtgcg tttggatttc cattggatgc ctcctgggtg
     1681 ttagccgcga gacatcagag gaaaccaggt agctgtgtcg attacctgac aggtaatgcc
     1741 ggcgtgccag tggaaaccag tataaaccag tacaaaccag ttcgaacact tatcccatcg
     1801 tcagcatgat cttgccgata tgcgcgctt
//
LOCUS       DASWQN010000021         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_134472, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000021 DASWQN010000000
VERSION     DASWQN010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134472"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VGC82_00345"
     CDS             complement(<1..1001)
                     /locus_tag="VGC82_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0421754.1"
                     /translation="MAAAATVKALGMALLLAAYATAAHAADAVQEPVYGAELQGFTYP
                     WPVAHFRFNSQGEALDMAYLDVTPKEPNGQVAVLLHGKNFCAATWQATITVLADAGYR
                     VIAPDQIGFCKSTKPSHYQYSFQQLAGNTHMLLASLGIAKTIMAGHSTGGMLAIRYGL
                     IYATEVSQLVLVDPIGLEDWKANGVPWQSVDEWYRQEKQNTAERIRDYERSTYYAGSW
                     QPAYETWVQMLAGMYRGPGRDVVAWNSALLYDMIYTQPVVYELPHLAMPVLLVIGDKD
                     TTAIGKALAPATIRPTLGNYPVLAKEAAATIPNAKLVEFPELGHAPQIQAPDAFHDAL
                     "
     gene            1146..1385
                     /locus_tag="VGC82_00350"
     CDS             1146..1385
                     /locus_tag="VGC82_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421755.1"
                     /translation="MSEQSNDTSKNPGADKDEQHETARQLTEDALGAYARGEREKGDK
                     MVEEAKHTDSSAIEEVIQDLDEDAGSDHTVPKGNG"
     gene            complement(1550..>2225)
                     /locus_tag="VGC82_00355"
     CDS             complement(1550..>2225)
                     /locus_tag="VGC82_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0421756.1"
                     /translation="EGRPRPIKMLRHETRFMNLLPHIPMYVSGFGPRAMALAGEHGDG
                     LVFAIPPRGVPVQDALSHARQGAARNGRDMTGFRNATLTNVALLQPGEAADSDRIKQT
                     IGPNVMASVYYFYDMVHETGMEPPEFLRRIWKPYCALVEQTPPEHRHFRTHEFHYTDL
                     HPGEAELIDEQLIRDVCLVGTADELIERIQGLERDGLQEMVFAVGNTAKWRLAEEFPR
                     TVMARL"
BASE COUNT          417 a          688 c          704 g          416 t
ORIGIN      
        1 agcgcgtcat ggaaggcgtc gggcgcctgg atctgcggcg cgtggccgag ttcggggaac
       61 tcgaccagtt tcgcattcgg gatcgttgcg gcagcttcct tggcgagcac cggatagttg
      121 cccagggtcg ggcggatcgt ggccggtgcc agcgccttgc caatggcggt ggtgtccttg
      181 tcgccgatga ccagcagcac cggcatggcg aggtgcggca gctcataaac caccggctgg
      241 gtgtagatca tgtcgtacag cagggccgag ttccaggcga cgacatcgcg gcccggaccg
      301 cggtacatcc cagccagcat ctgcacccag gtctcgtagg ctggctgcca actgccggcg
      361 tagtaagtgg agcgctcgta gtcccggata cgctcggcgg tgttttgctt ttcctggcga
      421 taccactcat cgacgctttg ccagggcacg ccgttcgcct tccaatcctc cagcccgatc
      481 ggatcgacca gtacgagctg actgacctcg gtcgcataga taagtccgta gcgaatggcc
      541 aacatcccgc cggtggaatg gccggccatg atcgttttgg cgatgccaag cgacgccagc
      601 agcatgtgcg tgttcccggc gagttgctgg aagctgtact ggtagtggct tggcttggtg
      661 gacttgcaga agccgatctg gtcgggcgcg atcacacggt agccggcatc cgccaggacc
      721 gtgatcgtgg cctgccatgt cgccgcacag aaattcttgc cgtgcagcag gaccgccacc
      781 tgcccgttcg gttccttcgg cgtcacgtcg aggtaggcca tgtcgagcgc ttcgccctgc
      841 gaattgaagc ggaaatgcgc cactggccac ggataggtga agccttgcag ttccgcccca
      901 tagactggct cctgaaccgc gtcggccgcg tgcgcggcgg ttgcgtaggc cgccagcagc
      961 agggccatgc caagagcttt gacggtagcg gcggcggcca tgttgaacct ttggtggaat
     1021 ctccgtcctc aacgcatcgc gtccaggcgc gttcgctctg acaatcccgg tggtgaccgc
     1081 gagcattggg aaccggcggc atcggccggc attcaggcat gatcgcagca accaggagcg
     1141 ccgcaatgag cgagcaatcg aacgacacct cgaaaaatcc aggcgccgat aaggacgagc
     1201 agcacgagac cgcccgtcag cttaccgagg atgcgcttgg cgcgtacgct cggggcgagc
     1261 gcgaaaaagg cgataaaatg gttgaagagg ccaaacacac agatagttcc gcgatcgagg
     1321 aagtcattca agatctcgat gaggatgccg gatccgacca caccgtaccc aagggaaatg
     1381 gctgacagtc tgctccgaaa cagtcgcttc gggcacggtt tagtcaccgc cttgccgggg
     1441 gtgtcctcgg gtcgaagggg ttatttgcca ccaagtcaac aagcctgcgc tggccgttcc
     1501 gatgggaagc gaacggccag cccattcgct gctgcgccgg cgggatagcc tacaacctgg
     1561 ccatcaccgt ccgcgggaat tcctccgcga gccgccattt cgcggtattg ccgacggcaa
     1621 ataccatttc ctgcagaccg tcccgttcca ggccctggat cctctcgatc aattcatccg
     1681 ccgtgccgac cagacacacg tcgcggatca gttgctcatc gatcaattcg gcctcgccag
     1741 gatgcagatc ggtgtaatgg aactcatggg tgcggaagtg ccgatgttcg ggaggagtct
     1801 gttccaccag cgcgcaatac ggcttccaga tgcgtcgcag gaattccggc ggctccatcc
     1861 cggtttcgtg gaccatgtcg taaaagtaat aaacactggc catgacattg ggaccgatcg
     1921 tctgcttgat ccggtccgaa tccgccgctt cgcccggttg cagcaacgcc acgttcgtca
     1981 gtgtggcgtt gcggaacccg gtcatgtccc ggccgttgcg cgccgcgccc tgcctggcgt
     2041 ggctcaaagc gtcttgcacc ggcacgcccc gaggcggaat ggcgaaaacc aggccgtcgc
     2101 catgttcccc agccagtgcc atcgcgcgcg ggccgaagcc ggatacgtac atgggaatgt
     2161 gcggaagcag gttcatgaac cgcgtctcgt gccgcagcat cttgattggg cgagggcgtc
     2221 cctcg
//
LOCUS       DASWQN010000022         4169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1190305, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000022 DASWQN010000000
VERSION     DASWQN010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190305"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..488
                     /locus_tag="VGC82_00360"
     CDS             213..488
                     /locus_tag="VGC82_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421757.1"
                     /translation="MNARRPPELDMTVEGEFVSPPTPPVSSRILMWAMVIAIVAGGLS
                     LAAFALWLALLILPVAFGAAVVAYVLFRYRVWRAQHSVSSQRNVWRR"
     gene            complement(496..1536)
                     /locus_tag="VGC82_00365"
     CDS             complement(496..1536)
                     /locus_tag="VGC82_00365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0421758.1"
                     /translation="MIRLSVLDQSTIVSNRSPDTSIRESIRLAQHCEALGYHRYWCAE
                     HHNSDSQAGTAPEILIAAIAATTHRIRVGSAGVMLPHYSALKVAEQFRVLDAIAPGRI
                     DLGLGRAPGSDGLTAYALNPRADTAADQFPANVRDLLAWLAGEKLLEGHPFRDIRAQP
                     SGPTMPEVWVLGSSDYGAQVAAYFGLPFCFAHFISDGQGAAQALGLFRDSYRPSVTHP
                     APHAAICVWAMAAETQEEAAYHFRSREMWRLNRDRGLFTALMSPQEAAALKVSESEQA
                     RLDRLRARSFYGTPGVVGNRLRALAAEHGVEEIAILTTLHDPEARRRSYSLLAEEFGL
                     AEELGLQVPRAE"
     gene            complement(1549..2835)
                     /locus_tag="VGC82_00370"
     CDS             complement(1549..2835)
                     /locus_tag="VGC82_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEY0421759.1"
                     /translation="MTRPDLQNLDPDILARRSLRNHRLFASGLLVLMAALTIATCFAP
                     RNWWSGLLEASARAGFIGGIADWFAVTALFRHPLGIPIPHTAIIPNQRGRLGQALGRF
                     VANHVFTSEEVSIVLRRLDLPGILHRFFADPGAAHPAAVALSGMLPKMLASVEDGRAR
                     RVLARVVPRIVGGPATGRIMARALHSLVDGGRHQEVFGFILSQLKNALASREDALRAA
                     IEERVREQGGRLVGWAMGASIARRVLGLINAELDKMSPDGSELRAAFDEWVRREINRM
                     ETEPERAAEVALALRRVVAHETVQAWMWDIWARIRSGVEADIARPNGRVVAYVEGALG
                     NIGTMLEADPAARDRLQIAAEGIIRSLLPSAQVRIAEFIGQVVASWDTATIVDRMELR
                     VGRDLQYIRMNGTIVGFLIGGLLYAVLDTLFGYTNF"
     gene            2997..3659
                     /locus_tag="VGC82_00375"
     CDS             2997..3659
                     /locus_tag="VGC82_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0421760.1"
                     /translation="MITLHDVELRHGTQIVLSGLSGRFESGSRTALIGANGSGKTTLL
                     RAIAGLHKLTSGQIDRDGLKQTDIALLAQGSHLDRSFPITCRDVVALAATHLGPFRSI
                     GNDRLTATNDALDRVGLAEMALRPIQALSAGQFQRILFARTIVQDAKLILLDEPFTAV
                     DAATTRLLLSVIAEWDAQGRTVIAVLHDIELVRRHFPHTLLLGHQSGQWDALPHSGLQ
                     AA"
     gene            3659..>4169
                     /locus_tag="VGC82_00380"
     CDS             3659..>4169
                     /locus_tag="VGC82_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY0421761.1"
                     /translation="MLDFLWEPFALGFMRRALAGCLALSAAAPPLGVFLVLRRMSLMS
                     DVLQHGVLPGIAVGAILTGLSVWAMGLGGIVAGFAITVVAGWLTRRTGGREDSQLAGM
                     YLMALAAGVALISTQRSIDLTHILFGNVLAVDDAALISMAGVATLTLPVLALIWRPLV
                     LESLDPGFMA"
BASE COUNT          715 a         1375 c         1301 g          778 t
ORIGIN      
        1 agcgattcaa gctctgtgcg gcttagctcg gaaagcgtga atgatgaagg cacatgcgca
       61 aaaaatcgca ccgagtcgag ccggagcaag cgaaaaatgc atggtcgccc ggagttttgc
      121 cccttttacc tttcatggtc gtactctcct gtgcatcgtc gcgcgctacg accggggttg
      181 aacccgaacc agtcgcgagc cacattcatc acatgaacgc acgtcgtccg cctgaactgg
      241 acatgaccgt cgaaggcgag ttcgtctcgc cgcccacgcc gccggtctca agccgcatac
      301 tgatgtgggc gatggtgatt gcgatcgtcg ccggcggtct gtcattggcg gcctttgccc
      361 tttggctggc actgctgatc ttgccggttg cgttcggtgc cgcagtcgta gcgtatgtgc
      421 tcttccgcta tcgcgtctgg cgagcccagc attcggtcag cagccagcga aacgtttggc
      481 gccggtaagc ttccgctact ccgcgcgagg gacctgtagt ccaagctctt cggcgagccc
      541 aaattcttcg gctaacaacg aataggatcg ccgccttgcc tcggggtcat gcagggtcgt
      601 caggatggcg atttcctcca cgccatgttc cgccgccagt gcgcgcaggc gattgcccac
      661 cacacccggc gttccataaa acgaacgcgc ccgcaaccgg tcgagccgtg cttgctcgct
      721 ctcggacacc ttgagcgcgg cggcctcctg cggtgacatc agcgcggtaa acaggccacg
      781 atcgcggttc aaccgccaca tttcgcgcga acggaaatga tatgcggctt cctcctgggt
      841 ttccgccgcc atagcccata cacagatcgc cgcgtgcgga gcagggtggg tgacgctcgg
      901 gcggtagctg tcccggaaca gtcccagagc ctgggctgcc ccctgtccat cgctgatgaa
      961 atgggcaaag cagaacggca gcccgaaata ggccgccact tgcgcgccgt agtccgaact
     1021 gcccagcacc cagacttccg gcatggtcgg tcctgaaggc tgagcacgga tgtcccggaa
     1081 cggatgcccc tccagcagtt tctcccccgc caaccaagcc agcaagtcac gcacattggc
     1141 cggaaattga tctgccgcgg tatccgcccg cgggttcagc gcataggccg tcagtccgtc
     1201 ggatcctggc gcccggccta gcccgaggtc gatccggccc ggcgcgatgg catccagaac
     1261 gcgaaactgc tccgccacct tcagcgccga gtaatgcggc agcatcacgc ccgccgatcc
     1321 tacgcgaata cgatgggtcg tcgccgcgat ggccgctatc aggatttccg gcgccgtccc
     1381 ggcctggctg tcagaattat ggtgctcggc gcaccaatac cgatggtagc ccaatgcctc
     1441 gcaatgctgg gccagacgga tgctttcgcg gatcgacgtg tcgggggagc ggttcgagac
     1501 gatagtcgat tgatccagaa ccgaaagccg tatcatgcgc cgctccgttc agaaattggt
     1561 ataaccgaac agcgtgtcca gcaccgcata gagcagaccg ccgatcagga agcccacgat
     1621 ggtcccgttc atgcggatat attgcaagtc gcgcccgacc cgaagctcca tccggtccac
     1681 gatcgtcgca gtatcccaac tcgcgaccac ctgaccgatg aattcggcga tgcgaacctg
     1741 ggcgctcggt aacagcgatc gaatgatgcc ctcggccgcg atttgcagcc ggtcccgggc
     1801 ggccggatcg gcttccagca tcgtgccgat attgcctaac gccccttcga cgtaagccac
     1861 cacccggccg ttcggcctgg cgatgtccgc ctccacgccg gagcggatgc gggcccagat
     1921 atcccacatc cacgcctgga cggtctcgtg cgccacgacc cgccggagtg ccaaggccac
     1981 ttccgccgcc cgctccggtt cagtctccat gcggttgatc tcgcgtcgta cccactcgtc
     2041 gaaggcggcg cgcagttccg atccgtccgg gctcattttg tccagttccg cgttgatcaa
     2101 ccctagcacg cgccgagcga tcgacgcccc catggcccag ccgaccagcc ggccaccctg
     2161 ttcgcgcacc cgctcctcga tcgccgcccg cagagcgtct tcccggcttg ccagcgcatt
     2221 tttcagttga ctgaggatga agccgaacac ctcctgatgc cgccccccat ccaccagact
     2281 gtgcagcgcg cgcgccatga tccggccggt ggcggggccg ccgacgatgc gcggcacgac
     2341 ccgagccagc acccgccgtg cgcggccatc ctccacgctc gccagcatct tgggcagcat
     2401 gccggacagc gccaccgccg cgggatgcgc cgcgcccgga tcggcgaaga accggtgcag
     2461 gatgccgggc aggtccagcc ggcgcagcac gatggaaact tcctcgctgg taaaaacgtg
     2521 attcgccacg aaccgcccca aggcctggcc gagacggccg cgctggttgg gaatgatggc
     2581 ggtatgagga attggtatgc ccaaaggatg ccgaaacagg gccgtcaccg cgaaccaatc
     2641 ggcgatgccg ccgatgaagc cagcgcgggc ggaggcctcc agcaagccgc tccaccagtt
     2701 ccggggcgcg aaacaggtcg cgatggtcag tgcggccatc agcaccagca agcccgaggc
     2761 gaacaggcga tgattgcgca gggagcgccg ggcgaggatg tcgggatcga ggttttggag
     2821 atcgggtcgg gtcatcgccg ccctgtttaa gcgcccggct cccttccgtc caacccggtg
     2881 gaatgttata ctgtaaccat gatcgaccca ttatccccga ctgccggcgc ggccgcccgc
     2941 cttttgctgg tgggtttggt gctgtgctgc atctgggcgg cggtaatttg ggcgctatga
     3001 tcacgctgca tgacgtggaa ttgcgccatg gcacgcagat cgtcctgtcg ggtttgtcgg
     3061 gacggttcga gtccggcagc cggactgcgc tgatcggggc aaacggctcc ggcaaaacca
     3121 cattgctgcg ggcgatcgcc gggctgcaca agttgaccag cggccagatc gaccgggatg
     3181 ggctgaagca aaccgatatc gcgctgctgg cgcagggcag tcacctggac cggtcgtttc
     3241 caataacctg ccgcgatgtg gtggccttgg ccgcaaccca cctggggccg ttccggtcga
     3301 tcggcaacga tcggctgacg gcgacgaacg acgccttgga ccgcgttggc ctggccgaaa
     3361 tggctttgcg tccgatccag gctttgtcgg cgggtcagtt tcaacgcatc ctgttcgccc
     3421 gcactatcgt gcaggatgcg aagctgattc tgctggacga acccttcact gccgtcgatg
     3481 cggcgacaac ccggctgctg ctttcggtca tcgcggaatg ggacgcccag ggccgcaccg
     3541 tcatcgcggt actgcacgat atcgaactgg tacggcggca ttttccgcac acgctgttgc
     3601 ttggccacca gtcgggccag tgggatgctt taccgcattc ggggctgcaa gcagcctgat
     3661 gctcgatttt ctgtgggaac cgttcgcgtt gggcttcatg cgccgcgcat tggcgggctg
     3721 ccttgctttg tcggccgccg cgccgccgct gggtgtcttc ctggtgctgc gccgcatgag
     3781 cctcatgagc gacgtgctgc agcacggtgt ccttcccggc atcgccgtcg gcgcgatcct
     3841 gaccgggcta tcggtttggg ccatgggtct gggcggcatt gtcgctggct tcgcgatcac
     3901 cgtcgttgcg ggttggttga cacgccgcac gggtggccgg gaggacagcc aactcgccgg
     3961 tatgtacctg atggcactgg cggcgggcgt tgcgttgatt tcgacgcagc gcagcatcga
     4021 cctgacccat atcctgttcg gcaacgtgtt ggcggtggac gatgccgcgt tgatcagcat
     4081 ggccggtgtg gcaacgttga cgcttcccgt gctggcgctg atctggcgtc ccctggttct
     4141 tgaaagcctg gaccccggtt tcatggcgg
//
LOCUS       DASWQN010000023         8069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2114197, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000023 DASWQN010000000
VERSION     DASWQN010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2114197"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(344..1657)
                     /locus_tag="VGC82_00385"
     CDS             complement(344..1657)
                     /locus_tag="VGC82_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008853755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY0421762.1"
                     /translation="MDTMVSDRPCLIDVRACRQAYHKDANADLVVLDDVNLTLHEGEI
                     VALLGRSGSGKSTLLRIVSGLLKPTSGSVTWRGTAVDGPTEGVAMVFQSFALFPWLTV
                     QENVEIGLEARGVHRAEREKRAETAIDMIGLGGFENAYPKELSGGMRQRVGLARALVV
                     HPDLLLMDEPFSALDVLTAETLRTDLIDLWMEGKLPVKSVLMVTHNIEEAVLMCDRIL
                     VFSSNPGRVANELKVPFPHPRNRLDPEFRQLVDDIYGIMTRRAPAGPKAATAIPVSPL
                     AMPLPRVGTNVMSGLMETLATEPYDGRADLPALAASLQYEVDDLLPLGEMLQLFRFAV
                     LEEGDIQLTDLGRSFVEADTEDRKRIFAIAMRQHVPLVNMIRQVLDERWNHRASAVRF
                     RDELEDHMSPDYAADTLRTVIGWGRYAELFSYDEEAEQFSLEDIE"
     gene            complement(1791..3518)
                     /locus_tag="VGC82_00390"
     CDS             complement(1791..3518)
                     /locus_tag="VGC82_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0421763.1"
                     /translation="MSASTYHSMAAGWRLPNVWDAVAFLCVIGALVGVGHVAEGTMIR
                     IDAPGATEITLDPTHLPEYAIRTTMRMFAALAASLLFTFTYGTAAAKRRRAALVLVPV
                     LDVLQSVPILGFLTFTVVFFMGLFPGQVIGLELAAIFAIFTSQAWNMAFSMYQSLKTV
                     PADLYEASISFHLTAWQRFWKLEVPFAAPGLVWNTMMSMSGGWFFVVASEAVSVGDNN
                     WKLPGIGSYVAVALERRDLVAVAWAILAMLVVILAYDQLLFRPLVAWSAKFRFETTAG
                     ATASDPWMLRLMRRTRLLKLFGEMVGRTASFVGGMRFSFTGRVRSAASRQPSRAVDAA
                     WIAVLLLLVAVAGWRVVLFASRGLTWGNVEDAVGLGLITLVRVVVLMILASLVWVPIG
                     VWLGLRPVWARRAQPIAQFLAAFPANLLFPPFVLFIVYFRLSPDIWLTPLMVLGTQWY
                     ILFNVVAGAAAFPGDLKEAASNFRIGGFLWWRKVIIPGIFPYYITGAITASGGSWNAA
                     IVAEVASWGDTKLRAHGLGAYIADATERGDMPRVVLGVVVMSAFVVLFNRLLWRPMYA
                     YAQRRLTLA"
     gene            complement(3628..5085)
                     /locus_tag="VGC82_00395"
     CDS             complement(3628..5085)
                     /locus_tag="VGC82_00395"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEY0421764.1"
                     /translation="MTLRCDLIIRDATVFDGLGNPRTRTDVGVTGDRIVAVGDLGGSE
                     ADRDISADGLALAPGFIDAHTHDDRAVLCGPECMLCKMSQGVTTVVVGNCGISLSPVR
                     MKSRPVPPLDLLGDESWFAFDSFAAYADRLARDPSPVNTYALIGHMSLRVEAMNGDTQ
                     RAATDQEAARMQARLRQALAEGASGFSTGLYYPPNMMAPTDEVIAVAEALRASGGLYV
                     THMRDEANDVLLSIEETLKIGRAVDVPVVISHHKCSMPENFGRSAETLPAIDKGAAGQ
                     KVDFDIYPYAAGSTVLMPHRLRQDVPVQITWSVPHPELAGRMLDDIAREWNLEPKAAA
                     ERLVPAGAIYFQMDEPDVQRIMAHRLAMIGSDGLPHDSYPHPRLWGTFPRVLGHYARD
                     LGLFSLEEAVRKMTGHTASVFGMVDRGVIRPGAYADLVLFDPATVRDLSTYDAPTRPA
                     QGILETWINGQSAYVFGQGATSARAGRLVTRNAAE"
     gene            complement(5569..6885)
                     /locus_tag="VGC82_00400"
     CDS             complement(5569..6885)
                     /locus_tag="VGC82_00400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0421765.1"
                     /translation="MDEAIEKSAMRKIYVRLLPFAIFTYFLAYIDRINVSFAGLTMQG
                     DLQMSAAAFGFAAGTFYWGYFIFEVPSNVIMEKVGARLWIARIMITWGIFAGITALVT
                     GSTSFAVVRFLLGVAEAGFFPGIVLYFTYWFPSHHHARIVSGFLIGLPIAVAGGAPIS
                     TAILSLDGLFGLRGWQIMYIAEAIPTILIGIVTLFVLTDRPEQAKFLTPEEKNWLAAK
                     LLAERRAKEAVKTFSMWQAMFDPKVLLLALNYFGIVVASLGMLIFIPQIIKSLGAGYG
                     NMAVGWLTMIPYICGGIALVVWGRVSDRMQERRWNLLGTCLVAAIGLFVAGYSMGTWW
                     VLVRMSIAAMGFYGSKGPFWAMPPMFLTGTGAAAAIAWINSIGNLGGFFGPWYVGFMK
                     DLTGSYTGGLYGLALLCLGSAIVCALFLHIPNVVAVRPQAVGSQAD"
     gene            7348..7674
                     /locus_tag="VGC82_00405"
     CDS             7348..7674
                     /locus_tag="VGC82_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421766.1"
                     /translation="MISDEPDDDDDLDDEAALDSYLGASDLPKRDAVSVELPDWPAPT
                     KRDVGLDLDIDTLAWFKQNHTDWRREMRFVLRAWMVAKMTQPRAALTLEHIIPAIEYD
                     RLQNRP"
     gene            complement(7704..>8069)
                     /locus_tag="VGC82_00410"
     CDS             complement(7704..>8069)
                     /locus_tag="VGC82_00410"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY0421767.1"
                     /translation="SKLRAAPAVKKRPRAGAPKAAPVARATAEAARAEAVRAEAARAE
                     WATDLVFDVREPKLAVSLRLDADVLRFFRGFGAGYQTRINEVLRAFMQARGGSEPIGR
                     AVVGARANRGAASNGKRAR"
BASE COUNT         1597 a         2651 c         2458 g         1363 t
ORIGIN      
        1 cacgacctgc aggaacaatc ctccggccca gttcgaagcc ttggcgaagc cgaccgccat
       61 cacggccacc agtaccgcac ccacccagaa gaccactcgg cgccaccaca ggcggggtga
      121 aaccagaggc atcctgccca cccggcgcag acgcatcagt cgcccgcttc gcccaacgtc
      181 catgatgttc cctgcgattt caattggcga cgccgccatg tccatcgtgt tccgaggcgg
      241 gcgatgctgt cgatacctgc tccggtatca cgcgccgggt tggcccccgc caggacggcg
      301 gaggaccggc ccgacgtgtc gatcttggtc gggaggtacc agtttactcg atatcctcca
      361 ggctgaactg ctctgcttct tcatcgtagc tgaacaattc ggcgtaccgt ccccacccga
      421 tgaccgtgcg cagcgtatcg gccgcgtagt ccggcgacat gtgatcttcc agctcatcac
      481 ggaaccgcac ggcggaggct cggtggttcc accgctcgtc cagcacctgg cggatcatgt
      541 tcaccagcgg cacatgctgg cgcattgcga tggcgaaaat ccgcttgcga tcctcggtgt
      601 cggcttccac aaaactgcgg ccgaggtcgg ttagctggat gtcgccctcc tccagcaccg
      661 cgaaccggaa cagttgcagc atttcgccaa gcggcagcag gtcgtccacc tcgtattgca
      721 gcgatgccgc cagcgccggc aggtcggctc gcccgtcata gggttcggtc gccagggtct
      781 ccatcagacc ggacatcacg ttggtgccca cacggggcag cggcatcgcc agcggcgaaa
      841 ccggtatcgc ggtcgcggcc tttggaccgg ccggggcgcg acgcgtcatg atcccgtaga
      901 tgtcgtcaac caactggcgg aattccgggt ccaggcggtt ccggggatgc gggaagggca
      961 ctttcaactc attggctacg cgtccgggat tggaggaaaa taccaggatg cggtcgcaca
     1021 tcagcaccgc ttcctcgatg ttgtgggtca ccatcagcac cgatttgacc gggagcttgc
     1081 cctccatcca caggtcgatc aggtcggtgc gaagggtttc cgccgtcagc acgtccaatg
     1141 cgctgaatgg ctcgtccatc agcaacaggt cgggatgaac caccagggcc cgagccaggc
     1201 cgacgcgctg gcgcatgcca cccgacaact ccttcgggta ggcgttttcg aacccgccca
     1261 gaccgatcat gtcgatcgcc gtttccgccc gtttctcccg ctcggcccgg tgcacgccgc
     1321 gtgcttccag cccgatctcg acgttttcct gcaccgtcag ccaggggaac agcgcgaagc
     1381 tctggaacac catcgccacg ccttcggtcg ggccgtccac cgcggtcccg cgccaggtca
     1441 ccgacccgga ggtcggtttc agcagccccg acacgatccg cagcaaggtc gattttcccg
     1501 aaccggagcg accgagcagc gccacgatct cgccctcgtg aagcgtgagg ttgacgtcgt
     1561 ccagcaccac cagatcggca ttggcatcct tgtgatacgc ttgccggcac gctcgcacgt
     1621 cgatcaggca aggccggtcg ctgaccatag tgtccatgac ggtgttccta aaaggatgat
     1681 cgggccggtg ggaggtccgg gccgcaggaa attcatagcc agcgcacagc cgtcacacca
     1741 atcccgagct tatcagtcgg ctcttcggcc gccggcatca agccaggata tcaagccagg
     1801 gttaggcgcc gctgcgcata ggcatacatg gggcgccaca gcaagcgatt gaacagcacg
     1861 acgaaagcgg acatcaccac cacgccgagc accaccctgg gcatgtcccc ccgttcggtg
     1921 gcgtcggcga tataggcacc cagaccgtgg gcccgaagct tggtgtcgcc ccatgacgcc
     1981 acctcggcga cgatggcggc gttccacgat ccgccggaag cggtgatcgc accggtgatg
     2041 tagtagggga aaatccccgg gatgatcacc ttgcgccacc acaggaaccc gccgatgcga
     2101 aaattgctgg cggcttcctt caaatccccg gggaaggcgg ccgcgccggc caccacgtta
     2161 aacaggatat accactgcgt acccagcacc atcagcggcg tcagccagat gtccgggctc
     2221 aggcggaagt acacgatgaa cagcacgaat ggcggaaaca gcaggttggc cgggaacgcg
     2281 gccaggaact gggcaatcgg ttgcgcccgc cgagcccaca ccggacgaag tcctagccag
     2341 accccgatcg gcacccagac caggctcgcg aggatcatca gcacgacgac ccgcaccaac
     2401 gtgatcagcc ccagcccgac ggcatcttca acattgcccc aggtcagacc ccgggaggcg
     2461 aacagcacca cccgccaccc cgccactgcc accagtagca gcagcaccgc gatccatgcc
     2521 gcatccacgg cccgggatgg ctgcctcgaa gccgccgacc gcacacgtcc ggtaaacgaa
     2581 aaccgcatcc cgccgacgaa ggatgcggtt ctgccgacca tttcaccaaa aagcttcagc
     2641 aaccgggttc gccgcatcag ccgcagcatc cacgggtcgg aggctgtcgc cccggcggtc
     2701 gtttcgaacc ggaatttcgc cgaccatgcc accagcgggc gaaacagcaa ttgatcgtat
     2761 gccaggatca ccaccagcat cgccaggatc gcccacgcga ccgcgaccag atcccgccgt
     2821 tccagcgcca ccgcgacata ggacccgatg ccgggcagct tccaattgtt gtcgccgacc
     2881 gacactgctt cggatgcgac gacgaagaac cagccgccgg acatcgacat catcgtgttc
     2941 cagacgaggc caggggcggc gaacggcact tccagcttcc agaaccgctg ccatgcggtc
     3001 aggtggaaac tgatcgaggc ctcgtacagg tcggctggga cggtcttgag cgactggtac
     3061 atgctgaagg ccatgttcca cgcctggctg gtgaagatcg cgaagatcgc cgccagttcc
     3121 agtccaatca cctgccccgg aaacaggccc atgaagaaca ccacggtgaa ggtcaggaac
     3181 cccaggatcg gcaccgattg cagcacgtcc agcaccggca ccaggaccag ggccgcgcgc
     3241 cggcgtttgg ccgccgcggt gccgtaggtg aaggtgaaca gcaacgacgc agccagggcg
     3301 gcaaacatcc gcatggtggt gcggatcgcg tattccggca ggtgggtcgg gtcgagggtg
     3361 atctcggtcg ctcctggcgc gtcgatgcgg atcatggtcc cctccgccac atgaccgacg
     3421 ccgaccagtg cgccgatcac gcacagaaac gcgaccgcgt cccaaacatt gggcaggcgc
     3481 catccggcgg ccatcgagtg gtaggtcgag gctgacatgt cgctgaaggg tgtttccggt
     3541 agggcgctga aagaaggccc tgttagcttc cccgtcgcaa ggtgtcacgc gcgaatggta
     3601 ttgctgttcg atgacgcaag gatcggatca ttcggctgcg ttacgggtaa ccagcctgcc
     3661 ggcccgggca ctggtcgcgc cttggccgaa aacataggcc gattggccgt tgatccaggt
     3721 ttccaggatg ccctgcgctg ggcgggtcgg cgcatcgtaa gtcgatagat cccgtaccgt
     3781 cgccggatcg aacaacacca ggtccgcata ggcgccgggc cggatgacgc cccggtccac
     3841 catgccgaag accgacgccg tgtgcccggt catcttgcgc actgcttcct ccaggctgaa
     3901 cagcccaagg tcgcgggcat agtgaccgag aacccgaggg aaggtgcccc acaggcgcgg
     3961 atgcgggtag ctgtcgtgcg gcaatccgtc gctgccgatc atcgccaaac ggtgagccat
     4021 gatccgctgt acgtccggtt cgtccatctg gaagtagatc gctccggcag gcaccagccg
     4081 ttcggcggcg gctttcggtt ccagattcca ttcacgagcg atgtcgtcca gcatccggcc
     4141 ggcgagttcc ggatgcggca ccgaccaggt gatctgcacc ggcacgtcct ggcgcagacg
     4201 gtgcggcatc agcacggtgg aacccgccgc gtacgggtag atgtcgaaat cgactttctg
     4261 cccggcggcg cccttgtcaa tcgccggcag ggtttcggca ctgcggccga aattctccgg
     4321 catcgagcat ttgtggtggc tgatcacgac tggcacatcg acagcacggc cgatcttcag
     4381 ggtttcctcg atcgacagca ggacgtcgtt cgcctcgtcg cgcatgtgcg tgacatacag
     4441 gccgcccgag gcgcgcaacg cctcggcgac ggcgatcacc tcgtcggtcg gcgccatcat
     4501 gttgggcggg tagtacagcc cggtggaaaa acccgaggca ccctcggcca gcgcctggcg
     4561 cagccgcgcc tgcatccggg cggcctcctg gtcggtggcg gcgcgctgcg tgtcgccgtt
     4621 catcgcttcg acgcgcagcg acatgtggcc gatcagcgcg taggtgttga ccggcgacgg
     4681 atcacgcgcg agtcggtcgg cgtatgcggc gaagctgtcg aaggcgaacc agctttcatc
     4741 ccccagcagg tccagcggcg gcaccgggcg ggacttcatc cgcaccggcg agagggagat
     4801 gccgcaattc cccacgacaa ccgtggtgac gccttgggac attttgcaca acatgcattc
     4861 cgggccgcac agcaccgccc ggtcgtcatg cgtgtgggcg tcgatgaagc ccggagcgag
     4921 cgcgaggcca tccgcactga tatcgcgatc cgcctcggac ccacccaggt cgccgaccgc
     4981 gacgatccgg tcgcccgtca cccccacatc ggttcgcgtc cgagggttgc cgaggccgtc
     5041 gaacacggtc gcatcgcgga tgatcaggtc gcagcgcagc gtcataagct tgttccgttc
     5101 atgcattggc cgagtagcgg tgaaagttta attggaacgt gcctttgtga ccatcgccgc
     5161 tctttgccgg ctgacggctg tgtgtctcca accggttgat tggccggcgc cccgttgagt
     5221 acacaatttg cgcataagag tggtatcgtg gctggtccgg agcatgaaac acacaagcat
     5281 tcacagtcgg tcggagtgcg aaccaggtgc tcttcggggg aagataagac tggcgccggc
     5341 cttcgataca cttccgcagg acgtccttgc cgccatggag ggccgggaag agtgaggatc
     5401 ctcctcgaca cccatgcttt cctttggtgg ctgggcgacg atgaccgaca cggcggacac
     5461 ggcttgcggt gctaaatctg gcacagacct ggatgatggg ccttcgccca ccatgacggg
     5521 atggttgggt tctgcatcaa tcggagcagt tgatcatgac tggacacctc agtccgcttg
     5581 cgaacccacc gcctggggcc gaaccgccac cacgttcggg atatggagaa acagcgcaca
     5641 gacgatggcg gagcccaggc acagcagggc cagcccataa aggccaccgg tgtagctgcc
     5701 ggtcaggtcc ttcatgaagc cgacatacca gggcccgaaa aatccgccga ggttgccaat
     5761 cgaattgatc catgcgatcg ccgccgcggc tccggtgccg gtgaggaaca ttggcggcat
     5821 cgcccagaac gggcctttcg agccatagaa gcccatagcc gcgatggaca tgcgaacaag
     5881 cacccaccag gtgcccatcg agtagccggc gacgaacaac ccgatcgccg cgacgaggca
     5941 tgttccgagc aggttccatc ggcgttcctg catcctgtcg gacacgcggc cccaaacgac
     6001 caacgcgatc ccgccgcaga tgtacgggat catcgtcagc cagccgacgg ccatgttgcc
     6061 atagcccgcg ccgagcgact tgatgatctg cgggatgaaa atcagcattc caagactggc
     6121 gacaacgatg ccgaagtagt tcagcgccag cagcagcacc ttcgggtcga acatcgcctg
     6181 ccacatactg aaggtcttca ccgcctcctt cgccctgcgt tcggcgagca acttggcagc
     6241 cagccagttc ttttcctccg gggtcaggaa tttcgcctgc tccggccggt ccgtcagcac
     6301 gaacaacgtc acgatgccga tcaggatggt cgggatagcc tcggcgatgt acatgatctg
     6361 ccaaccgcgc aggccaaaca ggccatcgag gctcaggatt gccgtcgata tcggtgcacc
     6421 gcccgcgacc gcgatcggca ggccgatcag gaacccggat acgatccgcg cgtggtgatg
     6481 gctggggaac cagtaggtga agtacaacac gatgcccgga aagaaaccag cttcggctac
     6541 acccagaagg aaccgcacaa ccgcgaaact tgtcgatccc gttaccaggg ccgtgatgcc
     6601 ggcgaagatc ccccaggtta tcatgatccg agcgatccac agccgcgccc cgaccttttc
     6661 catgatgacg ttgctgggca cttcaaagat gaaatagccc cagtagaacg tgccggccgc
     6721 gaaaccgaag gcggcggcgg acatctgcaa atcgccttgc atcgtcaatc cggcgaagct
     6781 tacgttgatg cggtcgatgt acgcgaggaa gtaggtgaag atggcgaacg gcaacagtcg
     6841 cacgtagatt ttgcgcattg ccgacttctc gatggcttcg tccatttgct ctcccggcgc
     6901 aaccttgcgg ccaccgatgc tccggcggcg cgggtttggc ttgattgaga caagacgtta
     6961 cgacatcggg cgctaccacg caatccggct cgttgccgcg aaaccaatca gcatgatcca
     7021 ttcagcccgg gccggcgaag cgcgaactgc aagcgcctga tcgtttgagc ggtttcacgg
     7081 tgactgggaa acggggtacc gttgtggtcg caaaggaagt cgtgggtggc cctcccaagc
     7141 ccggccgtga cgtttatgcg tcaggcagga aaccggcccg ccaccctttc caactagccc
     7201 cctggcgccc cgttcagtgc tatatagaac tattaacgat ccattaacgt ttctaagata
     7261 gtctgcgttg agcgcccgat cccgcgtcgc ctggcaagcg ggaccgcagg cgcttaatcc
     7321 tcaatttcag cacgagttcg atcaccaatg atttcagatg aaccggatga cgatgacgac
     7381 ctcgatgatg aggcagcatt ggacagctat ctgggcgcca gcgaccttcc gaagcgggat
     7441 gccgtttcgg tggagcttcc ggactggcct gcgccgacaa aacgcgatgt tggactggat
     7501 ctcgacatcg acacgttggc ttggttcaag cagaaccaca cggactggcg gcgggaaatg
     7561 cgctttgtgc tacgagcgtg gatggtcgcc aagatgacgc agccgcgtgc agccctgacg
     7621 cttgagcaca taattcctgc tatcgagtat gaccgactgc aaaaccggcc ataacacgaa
     7681 ctaaatgcgg tgcggccgat acgtcagcgc gcgcgcttgc cgttgctggc cgccccgcga
     7741 tttgcccgag cgccaacgac ggcacgccca atcggttcgg aaccgcctct tgcctgcatg
     7801 aaagccctca ggacctcgtt gatgcgggtc tggtaaccgg caccgaagcc gcggaagaag
     7861 cgaagcacat ccgcgtccag gcgcaggctc acggccagtt tcggctctcg gacatcgaac
     7921 accagatcgg tcgcccattc ggcacgcgcc gcttccgcac gcactgcttc cgcacgggcc
     7981 gcttccgccg tcgcgcgggc gaccggtgct gccttgggcg caccggcacg aggccgcttc
     8041 ttgacggccg gtgctgcacg cagcttgga
//
LOCUS       DASWQN010000024         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_926826, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000024 DASWQN010000000
VERSION     DASWQN010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926826"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1628
                     /locus_tag="VGC82_00415"
     CDS             <1..1628
                     /locus_tag="VGC82_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0421768.1"
                     /translation="LNQDLPLLLSSVLEHGATTHGAVKVIGRYANEAIDTDYAWLEAR
                     ARRLASALQRLGYGPDQFVGSLAWNTHRHLELFYGVSGVGAALHTANPRLPPAQIAYT
                     VNFTGYQTLFIDADTLALAEQLAPQLETVRRYVMMAPAGTLPPTSLPSLLSYEDLLAS
                     GDSGFTWPVLDERSACLLCFTSGTTGPPKGALYSHRGTVLNALSTGGGNAWGLSADDA
                     IIAIPGFFHCNGWAVPFFGPMYGARLVLPGRRADSGFLHALIVEHGITVAPGVPTIFL
                     DLLRHCHEMGAGLGRLNRLFSGGTAPPLAMMEAYLRDHGVRTIHGWGMTETTSGATMS
                     FAPHDLPPDAALAAMRTQGKPVFGAAIRIVGEDGGAAARDGITPGHLQVRGHWSAAGY
                     FRRPEVEAMTADGWLQTGDVATIEPDNTLHLIDRTKDVIKSGGEWISSQALEEAASRH
                     PAVREAAVIAIPHPRWQERPLLVVVAAAPVTEQDLRAHIATFVPKWWLPDAVVFIEAL
                     PHGPTGKLAKRELRQWLEEGRLGVVPAPMEPAG"
     gene            complement(2384..3154)
                     /locus_tag="VGC82_00420"
     CDS             complement(2384..3154)
                     /locus_tag="VGC82_00420"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0421769.1"
                     /translation="MRLQGKVALLTGAAAAIKGELMGFGGAAAHLFVREGAKVVLTDI
                     RDELGERAAAILRANGHDVRYMHLDVTSEQNWAQVVDAVMAAHGRLDILFNNAGVSFP
                     GSVEDTTVDIWDRELGVHAKGVFLGTRTAIPAMRKGGGGSIINTSSVMGIVGSPTSPA
                     YSAAKGAITLFTKSAALQYARENIRINSVHPGYADTPLTEKRFSDPAARQVLLDRTPM
                     GRLGTAEDIANGVLFLASDESSWVTGSELVIDGGMTAQ"
BASE COUNT          582 a         1125 c         1120 g          625 t
ORIGIN      
        1 ggcttaatca ggatctgccg ctgctgctgt cgagcgtgct ggagcatggc gccacgaccc
       61 atggcgcggt gaaggtgatc ggccgctatg ccaacgaggc aatcgacact gactatgcgt
      121 ggctggaagc gcgggcgcgg cggcttgcct cggcgctgca acggctgggc tatgggccgg
      181 accagttcgt agggtcgctg gcgtggaaca cccatcggca cctggagttg ttctatggcg
      241 tgtcaggcgt cggcgcggcg ctgcacaccg ccaatcctcg actgccgccg gcgcagattg
      301 cctacacggt caatttcacc ggctaccaga ccctgttcat cgacgccgat acgctggcgc
      361 tggcggagca gttggcgccg cagttggaga ccgtgcgacg ctacgtgatg atggcgccgg
      421 ccgggacgtt gccgccgact tcactgccat cgttgctgtc gtatgaggac ctgctggcga
      481 gcggcgactc gggcttcacc tggccagtgc tggacgagcg gtcggcgtgc ctgctgtgct
      541 tcacgtcagg caccaccggg ccgccgaagg gggcattgta cagccatcgc ggcacggtgc
      601 tgaacgcgct gtccaccggc ggcggcaatg cctggggact gagcgcggac gatgccatca
      661 ttgccatccc cgggttcttc cactgcaacg gctgggcggt gccgttcttc gggccgatgt
      721 acggcgcccg gctggtgctg ccgggacgcc gcgcggacag cggcttcctg cacgcgctga
      781 tcgtcgagca cgggatcacc gtcgcacccg gggtgccgac catcttcctt gatctgctgc
      841 ggcattgcca cgagatgggg gcggggctgg gccggctcaa tcggctgttc tccggcggta
      901 cggcaccccc gctggcgatg atggaggcat acctgcgcga ccacggtgtg cgcacgatcc
      961 atggctgggg catgaccgag acgaccagcg gtgcgacgat gtcgttcgcg ccgcacgacc
     1021 tgccgccgga tgcggcattg gcggcgatgc gcacgcaggg caagcctgtg ttcggcgcgg
     1081 cgatccgcat tgtcggtgag gacggcggcg cagccgcgcg cgacgggatc acgcccggcc
     1141 acctgcaggt gcgtggccac tggtcggccg ccggctattt ccgccggccg gaggtcgagg
     1201 cgatgaccgc ggatggctgg ctgcagactg gagacgtcgc aacgatcgag ccggacaaca
     1261 cgctgcattt gatcgaccgc accaaggacg tcatcaagtc gggcggcgaa tggatcagca
     1321 gccaggcgct ggaggaagcg gcctcccgcc atcccgcggt acgcgaggca gcggtgatcg
     1381 caatcccgca tccgcgctgg caggaacgtc cgctgctggt cgtggtcgca gcggcgccag
     1441 tcaccgaaca ggacctgcgt gcccacatag caacctttgt gccgaagtgg tggctgccag
     1501 acgctgtggt gtttatcgag gccttgccgc acggtccaac cgggaagctc gccaagcggg
     1561 agctgcgcca atggctggag gagggtcggc tgggggttgt tccggcgccg atggaaccgg
     1621 ctggataggg aggtcggtgt cgttgctcca gagaagtttg gatcccagta cctaccgctg
     1681 ctccagtccc cttccccaca aactttctcc gctggatttg gagccgctaa aaaggccagc
     1741 ttccgggcgg ttttgcgggt gaggcttggg gtttcaaagc cgaaacgata cgtgaattct
     1801 ctctccgaag cgcggtctct ctccggagct tggaggttgg ccgatttact ctacaagcgt
     1861 tttcttcagc attttcaata taattttatt tacaagtgac tgagaagttg gtgttgcgtg
     1921 cgctccctca aagctgcggc acgaaccttt tcttcggccc caagctgaag atcggcgtcg
     1981 ccgcgtcacc aatgcgtccc gcactctaca ggaaagcgat gcggcctcgc cgccattcca
     2041 ccgctgtcaa gcactgtatc ggctgcctgc tcccgacccc gcgcggtcct ccatggccca
     2101 gcccaatcgg ccggtatcga ccgagagcgg aatggcagcc ttcaagccac cggacgggaa
     2161 acctgccgtt cgttccggcc aaaccgttcg gccggtttcg agccaattcg gtcgcttgcc
     2221 caatcaaggc gacctcttcg aagcagacgc gtgggcaacg gtgcggcacg ggcaaccacg
     2281 gcagtgcagg tgttcagagc aaacaccacc tactttcctg atttggtcca cgggacggcg
     2341 ggccgcccag cggcgggctg cccggccgcg ggccgatttg cgcttactgt gccgtcatgc
     2401 cgccatcgat gaccagttcc gagccggtga cccaggatga ttcgtcggat gccaggaaca
     2461 gcacgccatt ggcgatgtcc tcggcggtgc cgagccgacc catcggcgtg cgatcgagca
     2521 gcacctggcg cgcggccgga tcgctgaagc gcttctcggt cagcggcgta tccgcatagc
     2581 ccggatgcac cgaattgatc cggatatttt cccgcgcata ttgcagcgcc gccgacttgg
     2641 tgaagagcgt aatggcgccc ttggccgccg aataggccgg cgaggtggga ctgccgacaa
     2701 tgcccatcac cgaggaggta ttgatgatcg acccgccacc gcctttgcgc atggccggga
     2761 tcgccgtgcg ggtgcccagg aagacgccct tggcatgaac gccaagctcg cggtcccaga
     2821 tgtccaccgt ggtgtcttcg acactgccgg gaaaggacac cccggcgttg ttgaacagaa
     2881 tatccagccg tccgtgcgcc gccatcacgg cgtcgaccac ctgggcccag ttctgttccg
     2941 atgtcacgtc gagatgcatg tagcgcacgt catggccgtt ggcgcgcagg atcgccgccg
     3001 cgcgctcgcc cagctcatcg cggatatcag tcagcaccac tttcgcgccc tcgcgcacga
     3061 acagatgcgc cgcggcgccg ccgaagccca tcaactcgcc cttgatcgca gccgccgcgc
     3121 cggtcagcag cgccaccttg ccctgcagtc gcatcctgtc ctcccatgtc attgttgatc
     3181 gccgggatga tgcgccattt cccatcctgc ctccaccgcc gcaccgcggc ccgatttgaa
     3241 ttgacgctcg cgcgcagtgg ttgcttccaa gctaaaggtt ccccgcggcg cttatggaaa
     3301 gctcagccca gcgatcgcat gactggcgga ccgtcatctg gccattccag gttccttgtg
     3361 ccaccgtgcg gcaaacacag ccggaggaat gccatgagcc agctcgaggt cgccgacaac
     3421 aaggtttcag cagatgcgaa tggaaccaag tc
//
LOCUS       DASWQN010000025         4840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1190762, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000025 DASWQN010000000
VERSION     DASWQN010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190762"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(410..1804)
                     /locus_tag="VGC82_00425"
     CDS             complement(410..1804)
                     /locus_tag="VGC82_00425"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0421770.1"
                     /translation="MISAPASEPLRRFLQVARGAGLRVSAAEGIDAARAVDIVGFEDR
                     TVLKDTLGLLLAKTPDEKAAYDEVFELYFKRDEFAAKDDLDESSSAERDPFSNSADGT
                     GGDGMGGQGGQSLGSLLAEDDRAALATAMEQAARDAGIENIRFFTQKNLYARRILDRM
                     GLRALERDLEAMRQSGTPEGLGRAQFLDGKIEALRDSVRDFVERNLLLFAKGDTEKFR
                     EELLKSTRLSNVERRDLERMRVLVRQMAKKLAARYAKTRRRRLRGQLDTRRTLRRNMG
                     WGGIPFITVWKQKRIEKPRVMVLCDVSGSVAAMAQFLLMFMYAINEALSDIRSFAFAG
                     SLIEVSEILEKEPVEEAITKIMSLIGFGSSNYGNCLADFEDGWMKFVTNKTTVIILGD
                     ARGNRTDPRTDVMGRLSQRSKRIIWLNPEYRSAWGTGDSDMFRYAPFCNLVTVCNTLR
                     HLERAVTDILEGAT"
     gene            complement(1801..2763)
                     /locus_tag="VGC82_00430"
     CDS             complement(1801..2763)
                     /locus_tag="VGC82_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0421771.1"
                     /translation="MNDAPPLGVLPLRSIDAVEYGLSEAGYIANRQISTAIYMAHHLQ
                     KPILVEGPAGVGKTELAKSVASWLKLPLIRMQCYEGLDESKALYEWKYGKQLLYTQIL
                     KDKINSVIGDADDLATALDKLHAFGDVFFSQQFLEPRPLLRSLQQPGGSVLLIDEIDK
                     SDQEFEAFLLEILSDYQVTIPEIGTVGALVPPIVLLTSNSTRDLGDALKRRCLHLHIG
                     FPDEKLEARIIASRVPGIDGRLLRQLVSFAQQLRALDLKKVPSVSETIDWARIMLLLH
                     ASVLEVDLVRDTLNVLLKFEADIEAAGKQLAGLTHKARQEAGVA"
     gene            complement(2842..3378)
                     /locus_tag="VGC82_00435"
     CDS             complement(2842..3378)
                     /locus_tag="VGC82_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0421772.1"
                     /translation="MIGGLRIRVHASDPVRRRGLLAMLEAAGHILTGETPDVELCDMD
                     RPSSPLPKEAEAPVVALTTGTPPGAPAGILPPDATAEQLDAALRAVFAGLLVRFATAP
                     EPRGFAPAEDVPLLTPREREILGLIGEGLSNKGMARRLGISVHTVKFHLEALFTKLDA
                     TSRAEAVTKGLRGGVIEL"
     gene            complement(3375..4262)
                     /locus_tag="VGC82_00440"
     CDS             complement(3375..4262)
                     /locus_tag="VGC82_00440"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEY0421773.1"
                     /translation="MDMIEQFSSGLADRIAAAASFTVGIQTGKRPVSGILWRPDVVVA
                     SEQMLPDDASALHVVRGGVRVDATLSGRDPSTNVAVLKLETPLDGALPETAEALRVGN
                     LALMVGADPAGNATGRLAMVHSLGEAWHSMAGGRIDAMIRLDGRLGADEGGPVLSLAG
                     TLIGMSTSGPRRRTIVIPTTTLARVIDPLLAEGRITRGWLGIGLQPVMIPDSFRPAAE
                     RENGLMVVGLAGGGPAETAGILPGDIVLDIDGAPAGRPRALTASLGPDRIGQTVALRV
                     LRAGGVQTVSVIIAARPAA"
     gene            4407..>4840
                     /locus_tag="VGC82_00445"
     CDS             4407..>4840
                     /locus_tag="VGC82_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0421774.1"
                     /translation="MPLDVLDHELDPYSAQVVQAFDRVGPAVVHVMAFAKDSRPVGQG
                     SGVIFTPDGYVLTNSHVVANAARLSASLTDGQSFDATLVGEDPATDIAVLRLSGTDLP
                     HAELGASASLKVGQLVVAIGNPLGFTCTVTAGIVSALGRTLRT"
BASE COUNT          866 a         1611 c         1493 g          870 t
ORIGIN      
        1 ccgggtgaga ccgaaccagg cggctgcggg aacctggggt ccgacacggc agccggccac
       61 gcggcgcggg caccgctcag ccgccagcga aagagcggca ccaagccgcg ggaggatgga
      121 gccacgttgg ggttgaagaa gtgttggccg tcgaaatggt ccgagagctt gtagcggttg
      181 gtcatcggcg gccgttagac cgcttcaggt cgaagggatc aagccttcgc attttatcga
      241 ggcgtttatt ccgcggcaac caggaatgcg tcgtgttcgg gggccacgat ggccggacgt
      301 tcgtgatcac cgatcgagga aagcgtcgca acgccatgcc gtcgcaagat tcccgcgatg
      361 gtaaaccgca tcgccgggca gccgaagctt ggcccgttgc cgttgctggc tacgtcgcgc
      421 cttccagaat atccgtgacc gcgcgctcca gatggcgcag cgtgttgcag acggtgacca
      481 ggttgcagaa cggcgcgtag cgaaacatgt cggagtcgcc cgtgccccag gccgaacgat
      541 attcggggtt cagccagatg atgcgctttg accgctgcga caaccgcccc atgacatctg
      601 tccggggatc ggtccgattg cccctggcat cgcccaggat gatcacggtc gtcttgttcg
      661 taacaaattt catccagcca tcttcgaaat cggcaaggca gttgccgtag ttcgaggacc
      721 caaatccaat cagcgacatg atcttggtga tcgcttcctc gaccggctct ttttccagga
      781 tttccgacac ctcgatcagc gatccggcga acgcgaacga ccggatgtcc gacagcgcct
      841 cgttgatcgc gtacatgaac atcagcagga actgcgccat cgccgcgacc gaccccgaga
      901 cgtcgcacag caccatcaca cgcggctttt cgatgcgctt ctgcttccat acggtgatga
      961 acggtatccc gccccagccc atgttccgcc gcagcgtccg ccttgtgtcg agttgcccgc
     1021 gtagccggcg gcgccgggtc ttggcatagc gggcagccag tttcttcgcc atctgccgca
     1081 ccagcacgcg catgcgttca aggtcacgcc gctccacgtt ggacagccgc gtcgatttca
     1141 gcagttcctc acgaaatttc tcggtatcac ccttggcgaa aagcagcagg ttccgttcga
     1201 cgaagtcacg aacagaatcg cgcagcgctt cgatcttccc gtccaggaac tgagcgcgtc
     1261 ccaggccctc cggcgtgccg gactggcgca tcgcctccag gtcgcgttcc agcgcgcgca
     1321 ggcccatccg gtccaggatg cggcgggcgt aaaggttttt ctgggtgaag aagcggatgt
     1381 tctcgatccc cgcatccctt gccgcctgtt ccatcgccgt ggccagggcg gcacggtcgt
     1441 cctcggccag cagcgaacca agcgactgac cgccctggcc gcccatgcca tccccgcccg
     1501 tgccgtcggc tgagttggag aaaggatccc gctcggcaga cgacgactca tcgaggtcgt
     1561 ccttggcggc gaattcatcg cgcttgaagt aaagctcgaa aacctcgtcg taagccgcct
     1621 tttcgtccgg cgtcttcgcc agcagcaggc ccagcgtgtc cttcagcacc gtccgatcct
     1681 cgaaccccac gatgtccacc gctcgggctg cgtcgatccc ctcggcagcc gatacgcgca
     1741 acccagcacc gcgcgccacc tgaaggaagc ggcgcaacgg ctcgctggcg ggcgccgata
     1801 tcatgcgacg cccgcttcct gcctcgcctt gtgggtcagg ccggcaagct gtttgccggc
     1861 ggcttcgata tccgcctcga atttcaacag cacgttcagc gtgtcgcgta ccagatcgac
     1921 ctccagcacc gaggcgtgca gcaacagcat gatcctggcc cagtcgatcg tctcgctgac
     1981 cgaaggcacc ttctttaggt ccagtgcccg aagctgttgc gcgaaactga ccagttgccg
     2041 caataaccgg ccatcgatgc cgggaacgcg ggaggcaatg atccgtgcct ccagcttttc
     2101 gtccgggaag ccgatatgca gatgcaggca acggcgcttc aaggcatcgc cgaggtcgcg
     2161 cgtgctgttg gaggtcagca gcacgattgg cggcacgagc gcgcccaccg tgccgatctc
     2221 cgggatcgtc acctggtagt cggaaaggat ctccagcagg aatgcctcga actcctggtc
     2281 cgacttgtcg atctcgtcga tcagcagcac cgaaccgcct ggctgctgca acgaccgcag
     2341 caagggccgg ggctccagga actgctgcga gaagaagaca tcgccgaacg cgtgcagctt
     2401 atccagcgcg gtggccagat cgtcggcatc gccgatgacc gagttgatct tgtccttcag
     2461 gatttgcgtg tagagcagct gcttgccgta tttccactcg tacagtgcct tggactcgtc
     2521 gagaccttcg tagcattgca tccggatcag cggcagcttc agccaggacg caacggactt
     2581 cgccagttcg gtcttgccga cgccagccgg cccctccacc aggatgggct tctgcagatg
     2641 gtgcgccatg tagatcgcgg ttgagatctg gcggttggcg atatacccgg cttcactcag
     2701 gccgtattcg acagcatcga tcgatcgcag gggcaacacc cccagcggtg gcgcgtcatt
     2761 cacatgcaac tccaggctac tgttcagtaa ccggatcgca tcgctgcgac ggaatgtcaa
     2821 acgcttgcga atggcaaccc gctaaagctc gatgaccccg ccccgcagcc ctttggtcac
     2881 cgcctccgcc cggctggtag cgtccagctt ggtgaacaag gcttccaagt ggaacttgac
     2941 ggtatggacc gagatcccaa gacggcgcgc catgcccttg ttactgaggc cttcaccgat
     3001 gaggcccagg atctcgcgct ctcgcggtgt cagcagcggc acgtcctcgg ccggtgcgaa
     3061 gccacggggt tcgggtgctg tggcgaagcg gaccagcagg ccggcaaaga ccgcccgcag
     3121 cgccgcatcc agttgctccg ccgtcgcgtc gggcggcaag attccggccg gggcaccggg
     3181 tggggtgccc gtcgtcagcg ccacgaccgg tgcctccgct tccttcggca agggcgaaga
     3241 cggccggtcc atatcgcaca gttccacatc cggcgtttcg cccgtgagga tatgcccggc
     3301 agcttccagc atcgccagca gacctcggcg gcgaaccggg tccgacgcgt ggacccttat
     3361 gcgaaggccg ccgatcatgc cgccggtcga gccgcaataa taacgctgac cgtttgcacc
     3421 ccaccggctc ggaggacccg cagcgccacc gtctgaccga tgcggtccgg gccaagggag
     3481 gcagtcagcg cacgcggccg gccggctggc gcgccatcga tgtccagcac gatgtcgccc
     3541 ggcaatattc ctgctgtttc agcgggaccg ccgccagcga ggccgaccac catcaggccg
     3601 ttttctcgtt cggcggccgg gcggaaacta tcgggaatca tcaccggctg cagtccaatt
     3661 cccagccacc cgcgagtgat ccggccctcg gccagcagtg gatcgataac gcgggcgagt
     3721 gtggtggtgg gaatcacaat ggtccggcgc ctcggcccgg atgtggacat tccgatcaga
     3781 gtgcctgcaa gcgacagcac cggcccgccc tcgtcggcac caagccttcc atccaggcgg
     3841 atcatggcgt cgatgcggcc accggccatg ctgtgccagg cctcgccgag cgaatgcacc
     3901 atcgccagac ggccggttgc attgccggct gggtcggccc caaccatcag ggcaaggtta
     3961 ccaacccgga gcgcttcggc cgtttcgggc agcgctccat ccaaaggcgt ctccaatttc
     4021 agcactgcga cgttggtgct gggatcgcgc cccgagagcg tcgcgtcgac gcgcacgccg
     4081 ccgcgcacca catgcagcgc cgacgcatcg tcgggaagca tctgttccga tgccacgacc
     4141 acgtccgggc gccacaggat accactgact ggccgcttgc cggtttggat gccgacggtg
     4201 aatgacgccg cggcggcaat gcggtcagcc aggcccgatg agaactgctc gatcatgtcc
     4261 atgcgttagc tcctttgcgc gacggcatca tgcgccggcc cgcagatcgg tggcatcggg
     4321 tggatgaccg ggcggacgcc tcgccagatg gccgggttca gcgacctttc cagcctcccc
     4381 atttgggggg cgaaaggaag acttccatgc cgctcgatgt tttagaccat gaactcgacc
     4441 cttattccgc gcaggtggtg caggctttcg accgcgttgg tccggcggtc gtgcatgtca
     4501 tggcgttcgc caaggatagc cgtccggtag ggcagggttc cggcgtgatc ttcacgcccg
     4561 atggctacgt gcttaccaac agccatgtcg tcgccaacgc cgcgaggcta agcgccagcc
     4621 tgaccgatgg acaaagcttc gacgccacgc tggttggaga ggatccggcg accgacatcg
     4681 ccgtactgcg cctttccggc accgatctgc cgcatgccga actgggcgcc tcggcgtcgt
     4741 tgaaagtcgg acagttggtg gtggcgatcg gcaatccgtt gggtttcacc tgcaccgtca
     4801 cggccggaat cgtcagcgcc ttgggccgta ccttgcgcac
//
LOCUS       DASWQN010000026         5504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2246507, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000026 DASWQN010000000
VERSION     DASWQN010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2246507"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..3882
                     /locus_tag="VGC82_00450"
     CDS             82..3882
                     /locus_tag="VGC82_00450"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEY0421775.1"
                     /translation="MGSRESTILTTDVVGSTALLRRYPNDMLAAMDLHDQILHAAIRR
                     RSGDPFRHTGDGVLAIFERPLDAVLAAIDAQREMRAAAWGPTGRLQIRYGIHTGLTRA
                     RPGGDFFGPSLPTASRLQSAANADQILLSGVTAEQLASDRLKTAFELSDLGEHHFKGI
                     EPIRVYQVRTSDLPSAFAPIAGKRETASGNLPANLSSFLGRERELADLAQMARESRLL
                     TLVGSGGIGKTRLAIEFARSLESTFPEGAWLVDLSALERDSDVWPAIAQALLIPPLPG
                     VEPRIQVLERLSDARAILLMDNCEHVLDPIADAVTELGSGCGSLFLINTSRRSLGVEG
                     EALYQVSSLDSGLQNGPGQSAAVRLFIERGRLADRTFQPTRDNLAVIQRICTHLDCIP
                     LAIEIAAGHLRRLPLESIESGVIKPLDLRSGNFRRRAGRQQTLRQALEWSYDLLEPNS
                     RQVLQRLSVFSGPFHEEQALAVCAADTLSESDVLRGIDELVESSLLTRDIGGHQLLRM
                     LQTVQAFGREKLDQTGLLQTVETRHGEVYAARCRELGQQIASVNEAKAASAIYDELSN
                     LRAAFDRAVTRNLKLAADLAAPLFLFNYFHRGAETGNWYERIMARPGADELEQAPILL
                     AGAAGHAFHDEGDQKKAAAFIERGFQSETDGSRSSDGWLSGVAGQMAQWSGDTTSCIK
                     YHATAVEQARRVGNSACEITSLCLAAFVKARTGDLDGAGELLREVTQTGQTAVQPTLM
                     GYIHYARGSVESFRDPNRAIEEYQTSEEWANMAGNHLGAQRVRQLIADLRAAQAAPSE
                     ALAIHLRTLKELPNHGATLYTWLTIRSLLLPLCELEADEDVAVLAGALHASPLKLNRA
                     AKNAVDKSKERLGDIAFELSAARGSGFGVTRARAYIIDVRESVGQQSSAAVSGEDLQE
                     SAGTAAMEGGMDISSEANSPEAERLVKAVISDFPDHKAGTRPVHTIGVGVEGHFVASD
                     VARHFCIAEHFRGDPVKATVRFSNGSGSPVQHDGWSDVRGMATRFHLPDGRATDLIAM
                     TLGEFFTPTVADFLAFTEASRPQPVQRRNPWRKILDLLQLRIPVPDPMPGEKASAGPG
                     MLGYANRHRFAQLAVSQAGGIGAPESYVRATYHAVHTFVVTGPDKVRRNVRFSWQPVA
                     GVRTNKPSTKDKYLRDELEKRLARWPARFMLMMTIGEAGDAFDDPTRPWPAKRVRVAM
                     GTLTLTKVADDQEATGERISFNPWRLLPGIEPSADPILHARRDAYEVSRQMRGGTRCP
                     FNRS"
     gene            3887..4603
                     /locus_tag="VGC82_00455"
     CDS             3887..4603
                     /locus_tag="VGC82_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421776.1"
                     /translation="MQDGRSWFERLVDGLPEKPLFQFLAARILVPYWAWRTPVKRQPG
                     GPHFSIQPGDNVQRMMNLVMPLKDKSAIGRAKAAGAIAANKDEIYAGLDNVGTVHFAR
                     FVIVDNNICMLSVYDGDFTNYIRDFIATIGSVFDAVVELVEGGEDVIPSEHNVEKFID
                     WVHAHDLYQVPDLATDVMRDQERLDGDNAAAGNDDLLLLGRRLILQLDVNKDVSLGSG
                     YRAYPGYSVAQVRKQLGIGW"
     gene            4600..>5504
                     /locus_tag="VGC82_00460"
     CDS             4600..>5504
                     /locus_tag="VGC82_00460"
                     /inference="COORDINATES: protein motif:HMM:NF044706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421777.1"
                     /translation="MSAAVFDTTDVQGNIVRGYRMPLVRYLVLEVADRTAARRWLADC
                     ISGRSDGVPQITLEAPWTTKPKCCFNIGLTYEGLRALGTPDRSLDSFPTEFKEGMSAR
                     DRALKLGDVGPSAPENWPAPFDDPRRVHLIATIYADETAELDRAQNQALGGGKAMKLL
                     GSRDGWSTRDNYVHFNYRDNLTQPRFAEVHDDERYEDGQPKAPLGTVLLGYENNFEGL
                     LWRVPYPDTLGTNGAFNAFRVLAQDVFGFEAYLDKAATDLLSHPKVDEVLSPGAEARI
                     GDGLSRHAALREVVAASMCGRWRNGV"
BASE COUNT         1050 a         1742 c         1738 g          974 t
ORIGIN      
        1 ttgggtacgg ggaaagagca gggctggttt tctttgacga aatacccatc caggaccggg
       61 tcggacgggg cggcttcacc agtgggcagc cgggaaagca cgattctcac gaccgatgtc
      121 gtgggcagca ccgccctgct ccgccgctat cccaacgaca tgctggctgc gatggatttg
      181 cacgaccaga ttctccacgc cgcgatccgc cgccgttccg gcgatccgtt caggcacacc
      241 ggcgacggcg tgttggcgat tttcgagcgg ccgctggacg cggtgctggc ggcgatcgac
      301 gcacaacgcg agatgcgcgc cgccgcgtgg ggcccgaccg gacggcttca gatacggtat
      361 ggcatccaca ccggcctgac acgggcgcga cccggaggcg acttcttcgg gccctcgctg
      421 ccaacggcct cgcggttaca gagcgccgca aacgctgatc aaatcctgct gtcgggcgtc
      481 accgcggagc agctggcgag cgaccgcctg aagacggcct tcgagttgtc cgacctcggc
      541 gaacaccact tcaaaggcat cgaaccgatc cgagtctatc aggtccggac atcggatctg
      601 ccgagcgcct tcgcaccgat cgccggcaag cgggagaccg caagcggcaa cctgcccgca
      661 aatctcagtt cattcctcgg ccgcgaacgc gaactggccg atcttgctca aatggcccgc
      721 gagtcgaggt tgctgaccct cgtgggctcc ggcggaatcg gcaagacgcg gctggccatc
      781 gagttcgccc gttccctgga atccaccttc cccgagggcg catggcttgt cgatctgtcg
      841 gccctcgagc gcgacagcga cgtatggccc gcgatcgcgc aggccctgtt gattccgccg
      901 ctgcccggcg tggagccccg aatccaggtg ctcgagcggc tgtccgacgc ccgggcgatt
      961 ctgctgatgg ataactgtga gcacgtgctc gaccccatag ccgacgccgt caccgaactg
     1021 ggctcgggct gtggctcgct gttcctgatc aacaccagcc ggcgctcgct cggcgtggag
     1081 ggcgaggcgc tctatcaagt ctcctcgctg gattcaggtc tgcagaacgg cccggggcag
     1141 tcggcggccg tccgcctgtt catagaacgg ggccggctgg ccgatcgaac gtttcagccg
     1201 actcgcgata atctcgccgt catccagcgg atttgcaccc atctcgattg cattcccctc
     1261 gccatcgaga ttgcggccgg tcacctgcgc cggcttcccc tggagagcat tgaaagcggg
     1321 gtgatcaaac ccctggacct gcgctcgggc aatttccggc gccgcgccgg ccgccagcaa
     1381 acattgcgac aggccctcga atggtcttac gaccttcttg agccaaacag ccgccaggtt
     1441 ctgcagcggc tgtccgtatt ctccggcccc ttccacgaag aacaggcgct ggctgtctgc
     1501 gccgccgata cgctcagcga atccgacgta ctccggggaa tcgacgagct cgtcgaatcc
     1561 tcgcttctga cgcgcgacat cggcggccat cagctgctga gaatgcttca aaccgttcag
     1621 gccttcggac gggaaaagct tgaccagacc ggcctgctcc agaccgtgga aacccggcat
     1681 ggcgaggtct atgcagcgag atgccgggag ctcggccaac aaatcgcgag cgtcaatgaa
     1741 gccaaggcgg cgagtgcgat ttacgacgag ctgtctaatc tgcgtgcggc cttcgaccgg
     1801 gcggtaacgc gcaatttaaa attggcggca gacctggcag cccctctgtt cctgttcaac
     1861 tacttccacc gcggcgccga aaccggcaat tggtatgagc ggatcatggc aaggccgggc
     1921 gccgatgagc tcgaacaggc gccgatcctc cttgccggag ccgccggcca cgccttccac
     1981 gacgagggcg atcaaaagaa ggccgccgct ttcatcgaac ggggcttcca aagcgaaact
     2041 gacgggtccc gatccagcga tggctggctt tccggtgtcg ccggtcagat ggcgcagtgg
     2101 tccggggaca cgacaagttg catcaagtat cacgcgaccg cggtcgagca ggcgcggcgc
     2161 gtcggcaaca gcgcctgtga gatcacgtcg ctatgtttgg cggcgttcgt gaaggcgcga
     2221 acgggagacc tcgatggcgc gggcgaactg ctcagggagg tcactcagac cgggcagacg
     2281 gccgtgcagc cgaccctgat gggatacatc cattacgcgc gaggcagcgt agaaagcttc
     2341 agggacccca accgggcgat cgaggagtac cagacgtccg aggaatgggc gaacatggcg
     2401 gggaatcatc tcggagctca acgggtcaga caattgatcg ctgatctgcg cgccgcgcag
     2461 gccgcgccga gcgaagcatt ggccattcat cttcgcacgt taaaggagct tcctaaccac
     2521 ggtgcaacct tgtacacttg gctgacgatc agatcgctgc tcctgccatt atgcgagctc
     2581 gaagccgatg aagatgttgc cgtactggcg ggtgcgctgc atgcttcacc cttgaagctg
     2641 aacagagcgg caaagaatgc tgttgataaa tccaaggaga gactgggcga cattgcattt
     2701 gagttgtccg ctgcccgcgg atccgggttc ggcgtaacgc gcgcgcgagc gtacattatc
     2761 gatgtccggg aaagcgtggg gcaacagtcg agcgcagccg tatctggcga ggatctccag
     2821 gaaagtgctg gcacggctgc aatggagggg ggcatggaca tttcatccga ggccaattcg
     2881 cccgaagccg agcggctggt caaagccgtc atttccgatt tcccggatca taaagccggg
     2941 accaggccgg tccacacgat cggcgtcggc gtggaagggc attttgtagc gtcagatgtc
     3001 gcgcgccatt tttgcatcgc cgaacacttc aggggagacc cggtcaaggc gaccgttaga
     3061 ttctccaacg gatcgggcag tccggtccag catgatggat ggtccgacgt ccgcggaatg
     3121 gcaacgcgtt tccacctgcc ggacggacgc gcaaccgacc tgatcgcaat gaccttgggg
     3181 gaattcttca cacccactgt cgccgacttt cttgcgttca ccgaggcttc gcggccgcag
     3241 ccggtgcaga ggcgaaatcc gtggcgcaaa atcctcgacc tgctgcagct caggattccc
     3301 gttcccgacc cgatgccggg agaaaaagcc agcgcgggtc cgggcatgct gggctacgcc
     3361 aatcggcaca ggttcgcgca actggccgtg tcgcaggccg gggggatcgg cgcccccgag
     3421 agctatgtcc gcgccaccta ccacgctgtc catactttcg tggtgaccgg tcccgacaag
     3481 gttcgtcgca acgttcgttt ttcctggcaa ccagtggccg gcgttcgcac caacaagccc
     3541 tcgacgaaag acaagtacct gcgcgacgaa cttgaaaaac gcctggcgcg gtggcctgcg
     3601 cgtttcatgc tgatgatgac gatcggcgaa gcgggcgacg cgttcgacga tcccacccgg
     3661 ccctggcccg ccaaacgcgt ccgcgtcgcc atgggcaccc tgacgctcac caaggtcgcc
     3721 gacgatcagg aagccacggg ggagcgcatc agttttaacc cgtggcgcct gttgcccggc
     3781 atcgagccct cggcggatcc aatcctccac gcccgccgcg acgcctacga ggtctcccgt
     3841 caaatgcgcg gcggcacccg gtgcccgttc aatcggagtt gagctgatgc aagatggcag
     3901 gagctggttc gagcgacttg tggatgggct gcccgagaaa cccctcttcc agttcctggc
     3961 ggcccggatc ctggtgccct attgggcatg gcgcacgccg gtaaagcgcc agcccggcgg
     4021 cccccatttt tccatccagc ctggcgacaa cgtccagcgc atgatgaacc tggtgatgcc
     4081 gctgaaggac aagtcggcga tcggccgtgc caaagccgca ggcgcgatcg cggcaaacaa
     4141 ggatgagatc tacgccggcc tggacaatgt cggcacggtg cactttgcgc gtttcgtgat
     4201 cgtcgacaac aatatctgca tgctctccgt ctatgacggg gatttcacca attacattcg
     4261 tgatttcatc gccacgatcg gcagcgtgtt cgacgccgtc gtggaattgg tcgaaggggg
     4321 cgaggacgtg attccttccg agcataatgt cgagaaattt atcgattggg tgcatgccca
     4381 tgacctgtat caggtgcccg atctggcgac cgatgtgatg agagaccagg aaaggctgga
     4441 cggcgacaat gcggctgccg gaaatgacga tctgctcctg ctggggcggc gtctcatcct
     4501 ccagctcgac gtcaacaagg acgtatcgct cggcagcggc taccgcgcct atcccggata
     4561 ttccgtagct caggtccgca agcagctggg gatcggctgg tgagcgccgc cgtgttcgac
     4621 accaccgatg tgcagggcaa catcgtgcgt ggctaccgca tgcccctggt acgatacctg
     4681 gttctcgagg tcgctgatcg caccgccgcc cgccgctggc tcgccgactg catatcgggc
     4741 cgctccgatg gcgttccgca gatcacgctg gaggcaccct ggacgaccaa gccgaaatgc
     4801 tgtttcaaca tcggcctgac ctatgaggga ctgcgggccc tgggcacgcc cgaccgctcc
     4861 ctcgatagct tcccgaccga gttcaaggaa ggcatgagcg cgagggaccg ggccttgaag
     4921 ctcggcgacg tcgggccgag cgcgccggaa aactggccgg ctcccttcga tgatcccagg
     4981 cgagtccatc tcatcgcgac catctatgcc gacgagacag ccgagctgga tcgcgcgcaa
     5041 aatcaggcgc tcggcggcgg caaggccatg aagctgcttg ggtcgcgcga cggctggagt
     5101 acgcgcgaca attacgttca cttcaactat cgcgacaacc tcacgcaacc gaggttcgcg
     5161 gaagtccacg acgacgaacg ttacgaggat gggcagccca aggcgccgct gggcaccgtg
     5221 cttttgggat atgaaaacaa tttcgaaggt ctgctgtggc gggttcctta tcccgacacg
     5281 ctgggaacca acggcgcctt caatgccttt cgggtgctcg cgcaggacgt gttcggcttc
     5341 gaggcctatc tcgacaaggc ggcgacggat ttgctcagcc acccgaaggt cgatgaagtg
     5401 ctgtcgcccg gcgcggaagc aaggatagga gacggcctct cacgtcatgc ggcgttgcgc
     5461 gaggtcgtcg ccgccagcat gtgcgggcga tggcggaacg gcgt
//
LOCUS       DASWQN010000027         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_927175, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000027 DASWQN010000000
VERSION     DASWQN010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927175"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGC82_00465"
     CDS             <1..234
                     /locus_tag="VGC82_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421778.1"
                     /translation="IYDDRVGETTRFTYRPNHEWYWFPQQKPTEVSMLKCYDSMTDGS
                     VSRWSFHTACIDPTAPADARCRKNVVVRSFVFF"
     gene            428..2068
                     /locus_tag="VGC82_00470"
     CDS             428..2068
                     /locus_tag="VGC82_00470"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0421779.1"
                     /translation="MNDRVESARESSAGSATDFDAIVIGAGVSGLYQLYRLRELGLTA
                     RVFEAGSNVGGTWYWNRYPGARFDSESWTYGYSFSQELLDEWDWEEHFAGQPETERYL
                     NHVADKFDLRRDIQFKTTVTAAHYQEDSRSWKVTLDNGASCTARFLITAVGVLSASVL
                     PRIPGLGSFKGQSCHTHNWPKEPVSFAGKRVAVIGTGATGIQTITEVAKTAGHLTVFQ
                     RTAQWSAPLHNSKVTPDEMREIRKNYADIFARCQETVGCFIHAADPRSVFEVTEEERE
                     AFWEKRYGEPGFGIWMGNFRDVLVDREANKLMSDYLARKIRQRVKDPAIAEKLVPKTH
                     GFGTRRVPLESGYFEVYNQPNVQLVDLTETPIERVTPEGIRTSDADYQFDMIIYATGF
                     DAITGSFDQIDIRGVGGVSLKDKWANGPVTLLGILVDGFPNFLMVMGPHAGLGNFPRA
                     VEYTSDWVTALIRYARDTGVTRMDATPEAAKAWTDHVIETNEGLLFTEVDSWMTGINR
                     NLEGKQVRRVMRYSGGHPAFRERCEAVSAGGYRELSMA"
     gene            complement(2140..>2454)
                     /locus_tag="VGC82_00475"
     CDS             complement(2140..>2454)
                     /locus_tag="VGC82_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY0421780.1"
                     /translation="IAAVEGIDMIAYGHSDLSARLGVHLQLEHPTFKAAVRRIAKACN
                     AHGKLARGSAETEAQIEEYWQLGCKVLNLPGNDVSTYLDGLKARASRAQARLKSIGVP
                     TP"
BASE COUNT          466 a          773 c          783 g          432 t
ORIGIN      
        1 atctacgacg accgggtcgg tgagaccacc cgcttcacct accggcccaa ccacgagtgg
       61 tattggtttc cccagcaaaa gccaaccgaa gtctcgatgc tcaagtgcta tgactcgatg
      121 accgatggct ccgtctcgcg ctggtcgttc cataccgcct gcatcgatcc gaccgcgcct
      181 gccgacgcca gatgccgcaa gaacgtcgta gtccggtcct tcgtcttctt ttaggaaaac
      241 gggagggcgc ggcgggcgcg ccgtgcgtcg caacggtacg ccggagcagt gggtggcgga
      301 ccagcggcga tccaggttat cctgccgcta aggtcgggga acctccggcc gcccgatctc
      361 aggcgggccg gcgggccggc tcaacgtcca aaggggccga gaggcttatc aggggagaca
      421 ggtgcgcatg aacgacagag tggaaagcgc gcgtgagtca tctgcagggt cggccaccga
      481 tttcgacgcg atcgtcatcg gcgccggtgt ttccggcctc tatcagctct accggctgcg
      541 tgaactcggc ctgacggcgc gggtgttcga ggctggctcc aatgtaggcg gtacctggta
      601 ctggaatcgt tatcccggcg cccggttcga ctccgaaagc tggacctatg gctactcctt
      661 ttcgcaggaa ctgctcgacg aatgggactg ggaggagcat ttcgccggcc agccggaaac
      721 cgagcggtat ctgaaccacg tcgccgacaa gttcgacctc cggcgcgaca tccagttcaa
      781 aaccacagtg accgcagcgc attaccagga agacagccga agctggaagg tcacgctcga
      841 caacggagcg tcctgcaccg cgagattcct gatcaccgcc gtcggcgtgc tgtcagcgtc
      901 tgttctgccg cgcattcccg gcctagggag tttcaagggc cagtcctgcc acacgcataa
      961 ctggcccaag gaacccgtca gttttgcagg caagcgggtc gcagtgatcg gcactggcgc
     1021 caccggcatc cagaccatca ccgaagtcgc caagactgcg ggccacctga ccgtgttcca
     1081 gcgcaccgcg caatggtcgg cgccgttgca caactccaag gtcacgcccg acgagatgcg
     1141 tgaaatccgc aagaactacg ctgatatctt cgcgcgctgc caggaaaccg tcggctgctt
     1201 catccatgcc gccgatcccc gctcggtgtt cgaggtgacg gaagaagaac gcgaggcgtt
     1261 ctgggaaaag cgttatggcg aaccgggctt cggcatctgg atgggcaatt tccgcgacgt
     1321 gctggtcgat cgcgaggcca acaagctgat gtcggactat ctggcgcgga agatccgcca
     1381 gcgagtcaag gatccggcaa tcgcggaaaa gctggtcccc aaaacgcacg gcttcgggac
     1441 gcggcgggta ccgctggaaa gcggctattt cgaggtttac aaccagccga acgtccaatt
     1501 ggtcgacctg accgaaacgc cgatcgagcg tgttacgccg gaggggatcc gcacctcgga
     1561 cgccgattac cagttcgaca tgatcatcta cgcgacgggg ttcgacgcga tcaccgggtc
     1621 attcgatcag atcgacatca ggggcgtggg cggcgtatcg ctgaaagaca aatgggccaa
     1681 cgggccggtg acgctgctgg gcattctcgt tgacgggttc ccaaatttcc tgatggtgat
     1741 gggcccgcac gccggcctcg gcaacttccc tcgggcggtg gaatacacgt ccgactgggt
     1801 caccgcgctg atccgttacg cgcgcgacac cggcgtgacc cgtatggatg cgacgcccga
     1861 ggccgcgaaa gcctggaccg atcacgttat cgaaaccaat gagggattgc tgttcaccga
     1921 ggtcgactcc tggatgaccg gcatcaaccg caacctggag ggcaaacagg tccgccgcgt
     1981 tatgcgctac agcggcggcc atccggcgtt ccgcgaacgc tgcgaagcgg tttcggcggg
     2041 aggctatcgg gaactgtcga tggcgtagcg tagcgtggcg tagcggtcag accggcggct
     2101 gccccggcga cgtttcgggt cccctgagcg ccgcgccttt cacggggtcg gcacaccgat
     2161 cgatttgagc cgggcctgtg cgcggctggc acgcgccttc aggccatcaa gatatgtgct
     2221 gacgtcgttg cccggcaggt tcagcacctt gcaccccaac tgccagtact cctcgatctg
     2281 cgcctcggtt tccgccgacc ctcgggcgag tttgccatgc gcgttgcagg ccttcgcgat
     2341 ccggcgcacg gcggccttga acgtcgggtg ctcaagctgg agatgaacgc cgaggcgcgc
     2401 actgaggtcg gagtggccat aggcgatcat gtcgatgccc tccaccgccg cgat
//
LOCUS       DASWQN010000028         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2114802, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000028 DASWQN010000000
VERSION     DASWQN010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2114802"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            419..724
                     /locus_tag="VGC82_00480"
     CDS             419..724
                     /locus_tag="VGC82_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421781.1"
                     /translation="MTRISSLFVATTLAILPLSAFAQTAPAKTAAPTNMTTTTNTTAP
                     AAGKTSEAAAGKTSQPAKPAVKDPAADTKTQVHGMNTVKTHHAKAPVSAKTADPLKS"
     gene            877..>1657
                     /locus_tag="VGC82_00485"
     CDS             877..>1657
                     /locus_tag="VGC82_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0421782.1"
                     /translation="MDAQATSLTIALPNRHAERRWLPAAWSGLLIVILSFLILYPTAM
                     LLIGALTTTNPVVEGYHLADLSIGNFLKVATNPNVASALGNSLIACGGGTIVAVVIGL
                     SFAWVVVRTNTPWKGLIASAGILPLFVPPLVAGVAWSILGSPKTGLLNLLAMKAGIPF
                     HIDLYTMPGMIFVFGIYYAPYVYMFTAAALRNMDPSLEEAAEISGASAARTMATVTFP
                     LILPAIISGMLLSFVVMLGIYGVPAVLGSPANISVLTTYIFT"
BASE COUNT          305 a          549 c          477 g          326 t
ORIGIN      
        1 gcgcctggtg gcggaatgat gccgtgccag ccctgcagcc cgatccgcca gagcggtttc
       61 acggtggctc gaaacagagt ctcctgtggc tgcgagaaag tcgtgggtgg ccgagccaag
      121 cacggccatg acacatcgac cgcaggcggc aaactggctc agatcctttc cagtcagcgt
      181 aaagggattc tgaccgcgcc gttacaggcc ggtcagcggc acagatttca tccaatggtt
      241 ggtcggtcca ctgtttcatc acgcggccgg tctgcgaagg tgtcggatta ttaatccgaa
      301 ttcgcaccgt ccgccacttt tccgccgccg gtccatctcg aaggcgtcac attcctatgg
      361 ccttatcctc ctcagccggg aacaaccaac gtatcccccg gcgaatagga aaacgaccat
      421 gacccgcatt tccagcctgt tcgtcgccac gacacttgcg atcctgccgc tcagcgcgtt
      481 cgctcagacc gcgccggcca agaccgccgc gccgaccaat atgacgacga caacgaatac
      541 gaccgcgcct gccgctggca aaacgagtga agccgcagcg ggtaagacga gccaacctgc
      601 caaacccgcc gtgaaggacc cggcagccga caccaagacc caggtgcacg gcatgaatac
      661 cgtcaagact catcacgcca aggcgccggt ttccgcgaaa accgccgacc ctctgaagtc
      721 ctaaccactg gacgcctcgg gcgaggcaac gcattccgcc tgtcgcttat gggacctggc
      781 cgccagcgcg gttgggttcc atttgcgttt ccgccgttgc acttcctcgc gcgccatgtg
      841 acacgaccct ccggaaggat gccggaggaa ccaagagtgg atgcacaggc caccagcctg
      901 accatcgcgt tgccgaaccg gcacgctgaa cgtcgttggc tgccggcggc atggtccggc
      961 ttgctgatcg tcattctgag cttcctgatt ctttatccca ccgcgatgct gctgatcggt
     1021 gccttgacca caaccaatcc ggtggtcgag ggctaccacc tcgcggacct ctcgatcggc
     1081 aatttcctga aggtcgcgac caacccgaat gtcgcttcgg ccctggggaa ttcgctaatc
     1141 gcctgcggcg gcggcaccat cgtcgcggtg gtcattgggc tgtccttcgc ctgggtggtc
     1201 gtgcgcacaa acacgccgtg gaagggcctg atcgccagcg ccggcatact gccgctgttc
     1261 gtcccgccgt tggtcgcggg tgtcgcatgg agcatcctgg ggtcgccgaa aaccgggctg
     1321 ctgaatttgc tggcgatgaa ggctggaatc ccctttcaca tcgacctcta caccatgccg
     1381 gggatgattt tcgtcttcgg catctactac gcgccttacg tttatatgtt cactgcagcg
     1441 gcgctgcgga acatggaccc gtcgctggag gaagccgccg agatttccgg tgccagcgcc
     1501 gcgcgcacca tggcgaccgt gacgtttccg ctgatcctgc cggcaatcat ctccggcatg
     1561 ctgctgtcgt tcgttgtcat gctgggtatc tacggcgtgc ccgcggtact gggatcgccc
     1621 gcgaatattt cggtgctgac gacttacatc ttcaccc
//
LOCUS       DASWQN010000029         2828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1191124, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000029 DASWQN010000000
VERSION     DASWQN010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191124"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VGC82_00490"
     CDS             <1..743
                     /locus_tag="VGC82_00490"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY0421783.1"
                     /translation="HRASWERMGNPLFLIPPLAAIVIPVGLILKEPNLGTAAITAVLG
                     GVMFFAAGVRWWKFVLVVLPIPLLGQLAYTHLHEYQRARIDTFLNPDSDKLGAGYNII
                     QSKIALGSGGMWGRGFLQGTQAHLDFLPEKQTDFIFTMIGEEFGFAGGIALMALLGLI
                     IVGGMLIALRCRHSFGRLVALGISCNFCLYVFANIAMVMGVIPVGGVPLPLVSHGGSA
                     MLTVMVGFGVLMSVHVHRDVGFAAADER"
     gene            987..1589
                     /locus_tag="VGC82_00495"
     CDS             987..1589
                     /locus_tag="VGC82_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421784.1"
                     /translation="MSRSPSKAAIAAEERGLLERQRVFRSVADRLAVALSRCQAVEAI
                     ALIGSVARPLRREVPRFSPYRQLRLPIAHECKDVDLAAWVSRLDGLEELRRVRVAQDI
                     VAENGYGPAVQEMEVFLLEPGTNRYLGRVCHFRQCPAAKRDCLATGCGREPFLKQHEE
                     FSFWPQAIADGMAIRLYDRTGGGMIAHAADLPSVILDAGP"
     gene            complement(1831..2202)
                     /locus_tag="VGC82_00500"
     CDS             complement(1831..2202)
                     /locus_tag="VGC82_00500"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0421785.1"
                     /translation="MKTDTTLQSDHDTLVALNRDYIASVQQGDVQRFEDILADDFLCS
                     NPDGSLLDKAQFLTQTAQPVTISFLVAEDVRIRLLGDIAIIHACTRYHTANGEQRQGR
                     YTDVWARRGTRWLAVSAHVTR"
     gene            complement(2427..>2828)
                     /locus_tag="VGC82_00505"
     CDS             complement(2427..>2828)
                     /locus_tag="VGC82_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0421786.1"
                     /translation="GMNVEIQEMDWNTLAQRRTKADPIDKGGWSIFHNWWLGTSIANP
                     AISTVVRGLGAKGWAGWYSNEKIEALTADWTHASTDEARHSLARSIHQEAMNSVPTVV
                     LGRFFILTAHRKGLRGLLQGTSPYPWNLSWT"
BASE COUNT          474 a          879 c          875 g          600 t
ORIGIN      
        1 ttcatcgcgc atcctgggag cgaatgggca acccgctgtt cctgatcccg cccctggccg
       61 ccatcgtgat ccccgtcggc ctgatcctga aggagcctaa cctgggcacc gcggcgatca
      121 ccgccgtgct gggcggcgtg atgttcttcg ccgccggcgt ccgctggtgg aagttcgtcc
      181 tggtggtgct gccgatcccg ctgcttgggc aactggccta tacgcatctg cacgagtacc
      241 agcgtgctcg gatcgacaca ttcctgaatc cggacagcga caagctgggc gccggctaca
      301 atatcattca gtcgaagatc gccctgggct ccggcgggat gtgggggaga ggattcctgc
      361 agggcaccca ggcacatctg gattttttgc cggagaagca gaccgacttc atcttcacga
      421 tgatcggcga ggagtttggc ttcgctggcg gcatcgcgct gatggcgctg ctgggcctga
      481 tcatagtcgg gggaatgctg atcgcgctgc gttgccggca ctcgttcggc cggttggtgg
      541 cgctgggcat ttcctgcaat ttctgcctct atgtgttcgc caacatcgcc atggtcatgg
      601 gcgtgatccc ggttggcggc gtgccgctgc cgctggtgtc ccatggtgga tcggcgatgc
      661 tgacggtcat ggtcgggttc ggcgtgctga tgtcggtgca tgtgcatcgc gacgtggggt
      721 tcgctgccgc cgatgagcgg tagaggcagg ctgtcacgac atggcggttg tcaaagcgcc
      781 cctgcttcag ggcaaggcgg cgccagactg gccatgcatc cacccaggca ttaataaggt
      841 cgaaccggcc cgaccgaatc taaccccgat caggatggtt cggaggccgg cgatgctgtt
      901 tgaaacaggc ggcgtcctcg gttcttggga cgatatcaag agtcacggca cgaccgcgga
      961 ggcatgttgg tgccataaag gaggatatgt cccgctctcc aagcaaagcc gctattgccg
     1021 ctgaggaacg cggactgctc gaacggcagc gggtttttcg ttccgtggcc gatcggcttg
     1081 cagtcgcatt atcgcgatgc caggctgtcg aggccattgc gttaatcggc tctgttgccc
     1141 gcccgcttcg gcgcgaggtg ccacgttttt caccctacag gcagctacgc ctgccgatcg
     1201 cccatgagtg caaggacgtc gatcttgccg cgtgggtttc gcgactggac ggattagaag
     1261 aactgcggcg tgttcgcgtc gcccaggaca tcgtcgccga gaacggttat ggaccggccg
     1321 ttcaggaaat ggaggttttc ctgctcgagc cgggcacaaa ccgctatctc ggccgagtct
     1381 gccactttcg gcaatgcccg gcagccaaac gcgattgtct cgccaccgga tgcggccgcg
     1441 agccattcct gaagcaacac gaagagtttt cgttctggcc gcaagctatc gccgatggca
     1501 tggccattcg gctctacgac cgcaccggtg gcgggatgat cgcgcatgcc gccgacctgc
     1561 cttcagtcat ccttgatgct ggtccctgag aagtcggccg cttggtgaag gctgctttcg
     1621 accgggttgc cgatcgtttc gcgctggcgc tggcgccacg tccacggctg ctttcgaagc
     1681 atggcaagcg acagacctgc gcggtatctt ctcgcctccg cccgaaagac catgatcgtt
     1741 tgaggttgaa ccctccacca cgtccgatca tggtctaacc cgatgtgatc gcgtgattcg
     1801 cgccttctga ctgggtcaac ccctggccgg tcaacgcgtc acatgcgccg acacggcaag
     1861 ccatcgcgtg ccgcgacggg cccacacatc ggtatagcgc ccctgtcttt gttcgccgtt
     1921 cgcggtgtgg tagcgggtgc aggcgtgaat gatggcaata tcgccgagca ggcggatccg
     1981 gacatcctcc gcgacaagga agctgatcgt caccggctgt gctgtctgtg tcaggaattg
     2041 agccttgtcg agaagtgagc cgtccgggtt cgagcacagg aaatcgtcgg ccagaatgtc
     2101 ctcgaagcgt tgtacgtcgc cctgctgaac cgatgcgatg taatcgcggt tcagcgcaac
     2161 cagcgtgtca tgatcgctct gcagggtggt gtcggtcttc attggcgttc tcctgatggc
     2221 gacgcttcgg caatcctgac acggccgcca actactgaaa tgtcccagcg ttaccgcgaa
     2281 ggctggccct gaccttcgcc cctgtgtccg cagcagcacc gcccgcgtgt gtcgttctca
     2341 acgctgtgtt tacgcaacac cacggaccat acgtcttgta tgccggcccg cgcgatccac
     2401 cgcacgcacc cgaacggcgt tcagcctcat gtccaggata ggttccacgg gtagggggaa
     2461 gttccttgca gcagtccgcg caatcccttc cggtgtgccg tcaggataaa gaaccgtccc
     2521 aacaccaccg tgggcaccga gttcatcgct tcctgatgga tcgaccgcgc caggctgtga
     2581 cgggcctcat ccgtcgatgc gtgggtccag tcggctgtca gcgcctcgat cttctcgttg
     2641 ctgtaccacc cggcccagcc cttggcgccc aacccgcgca ccaccgtgct gatggccgga
     2701 ttggcgatcg aggtgcctaa ccaccagttg tggaagatcg accacccgcc tttgtcaatt
     2761 ggatcggcct tcgtgcgccg ttgcgccagg gtgttccagt ccatttcctg aatttcgacg
     2821 ttcatgcc
//
LOCUS       DASWQN010000030         4759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_135500, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000030 DASWQN010000000
VERSION     DASWQN010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135500"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VGC82_00510"
     CDS             complement(<1..355)
                     /locus_tag="VGC82_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY0421787.1"
                     /translation="MPKVLISDKLSPAAVEIFRDRGIEVDLKPGLSPAELRAIVGAYD
                     GLAIRSATRVTKELLDAAVNLKVVGRAGIGVDNVDVAAATKRGVMVVNAPQSNILSAA
                     EHTVALLLAQARTIPQ"
     gene            complement(370..1545)
                     /locus_tag="VGC82_00515"
     CDS             complement(370..1545)
                     /locus_tag="VGC82_00515"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEY0421788.1"
                     /translation="MANTATPDIRPSNPNFSSGPCAKRPGFTLEALTGAFLGRSHRAA
                     GPKARLAEVISLSRSVLGMPAGWRLGIVPGSDTGAVEMALWSMLGARGVDIVTFESFG
                     EGWATDVVRHLKLSDARVLSAAYGALPDMAAVGPDRDVVFPWNGTTSGVRVPNADWIA
                     ADRRGLAICDATSAAFAMALPWDKLDVVTWSWQKVLGGEGGHGMLALSPRAVERLQTY
                     TPAWPLPKIFRLTSNGKISEGIFAGETINTPSMLCVEDAIDGLRWAERIGGLPALIAR
                     SEANLAAVAAWIERSDWAAFLAEDPATRSCTSICLKITAPWFVALPADGQAKAAKQLA
                     ALLEKQGVAYDIASYRDAPPGLRLWAGATVETSDIEALLPWLDWAAARVAAIFAPAQ"
     gene            1904..2242
                     /locus_tag="VGC82_00520"
     CDS             1904..2242
                     /locus_tag="VGC82_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421789.1"
                     /translation="MPRNPSRTGTTIVLAALVAATSPTLAQNPPGASPANPTVPVAPP
                     AAPAAPAEKIVPPDGGTPSNRLSRQGGVINPPNIDPGMTVNPPKSGSTTTPVIPPPGS
                     PGGNPSVVPK"
     gene            complement(2225..3310)
                     /locus_tag="VGC82_00525"
     CDS             complement(2225..3310)
                     /locus_tag="VGC82_00525"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0421790.1"
                     /translation="MKSEIDEAVACALSSGWYILGNELRAFEAEFAAWLEVSSVIGCG
                     NGTDAIALALRALDIGSGCSVVTVSHTAVATVAAIEMTGATPLLIDIDPARYTMDPGA
                     LRFVLENPPPDLAPIKAVIPVHLYGQPADLDQIVPLCRKHGVALIEDCAQAHGARLHG
                     QRVGTFGDAATFSFYPTKNLGALGDGGAVAVSDPNLAERVAALRQYGWHKHYISNEVG
                     VNSRLDEIQAAILRVKLPHLDRQNARRAEIAAAYTAALAGGTLLPPRQSDGVSHVFHL
                     YVTRSAHRDRLQARLKADGVGTGIHYPVPVHLQPAYAGRVALGPGGCRETERTATEIL
                     SLPVYPELTDLEIATICAALRALLRDN"
     gene            complement(3346..4317)
                     /locus_tag="VGC82_00530"
     CDS             complement(3346..4317)
                     /locus_tag="VGC82_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0421791.1"
                     /translation="MNWSGRKVFVTGGAGFLGSNLCHALAARGARVTALDGFLFGGGA
                     NPRNLDGADVDFVRGDIRELDLRPLCEGAEVIFNLAAQTSHMGGQADPLADIAVNAVA
                     QVRLIQAAREAAPDAIVVHASTRQFYGRVEKLPVDENQPVAPPDANGVSKFAGEQYWM
                     LENRVRNRPVVSLRLTNCYGPRLRIRDARQTFLGIWIRCVLEHRPFEVWGGAQLRDLT
                     YVDDVTDAFLAAAEQPSCHGGIFNIGGSPPASLQELADLVVRLSGGTGSYTTREFPAD
                     RARIDIGSYHADDSAFRAASGWKPETSLQDGIGRTLDWYRTRLADYL"
     gene            complement(4401..>4759)
                     /locus_tag="VGC82_00535"
     CDS             complement(4401..>4759)
                     /locus_tag="VGC82_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY0421792.1"
                     /translation="DPGFMAATTRSGGIWHLIFLMLVVLCVVSGFVALGTLMSVGLMM
                     LPAISARHWSDSLAGQVRVAVLLACGSSAGGLILSFNLDIPAGPAIVLTAGGLWVASL
                     LTGPHASLWRRAMPTA"
BASE COUNT          931 a         1546 c         1519 g          763 t
ORIGIN      
        1 cttgggggat ggtgcgcgcc tgcgcgagca aaagggcgac ggtgtgctca gcggcagaga
       61 ggatgttcga ctgcggggcg ttgacgacca tgacgccgcg cttggtggca gcggccacat
      121 cgacgttgtc cacgccgatc ccggccctgc cgacgacctt cagattcacg gctgcatcca
      181 ggagttcttt cgtcaccctg gtggcggaac ggatggccag cccatcatag gctccgacaa
      241 tggcgcgcag ttcggcgggc gacagacctg gtttgagatc cacttcgatt ccacgatcgc
      301 gaaaaatctc gacagcagcc ggcgacagct tgtcgctgat caataccttg ggcatatcaa
      361 gtcctatcgt tactgagcag gagcgaatat ggcggcaacc cgggcagcgg cccagtcgag
      421 ccatggcaac aatgcctcga tgtcgctggt ctccacggtg gcgccggccc agaggcgcaa
      481 cccgggcgga gcgtcgcggt aggacgcgat gtcgtaagcg acaccctgct tttccagcag
      541 cgcggccagt tgcttcgccg ccttcgcctg accgtccgcc ggtagcgcga caaaccacgg
      601 agcggtgatt ttcaggcaga tggaggtgca agagcgggtg gcgggatcct ctgccagaaa
      661 ggcggcccag tcgctgcgct cgatccaggc cgcaacagcg gccagattgg cctcagagcg
      721 ggcaatcagc gccggaaggc cgccgatacg ttcggcccag cgcaagccgt cgatcgcgtc
      781 ctccacacac agcatcgacg gggtgttgat cgtttcgcct gcaaaaatgc cctcggaaat
      841 cttgccgttg gaggtcaggc ggaagatctt cggcaatggc cacgccgggg tgtaggtttg
      901 cagccgttca accgcgcgcg gcgacaaagc aagcatgccg tgcccacctt ccccgcccag
      961 cactttttgc cacgaccatg tcaccacatc cagcttgtcc cagggcagcg ccatggcaaa
     1021 ggcggccgaa gtggcatcgc aaatcgccag gccccgccgg tctgcggcga tccaatcagc
     1081 atttggcact cgcacaccgg acgtcgtgcc gttccacggg aagaccacgt cccggtcggg
     1141 accgaccgca gccatatcgg gaagcgcgcc ataggcggcc gacagcacgc gggcgtcgga
     1201 caacttcagg tgcctgacga cgtcggttgc ccaaccctcg ccgaaacttt caaaggtcac
     1261 gatatcgacg ccccgtgcgc ccagcatcga ccatagcgcc atttcaacag caccggtgtc
     1321 cgacccgggg acgattccca gccgccatcc ggcgggcatg ccgagaacgg aacgtgaaag
     1381 cgaaataact tcggccagcc tggcctttgg tccggcggca cggtggctgc ggccgaggaa
     1441 ggcgcccgtc aaggcctcca gggtgaagcc cggccgcttg gcgcatgggc ccgatgaaaa
     1501 attggggttt gacggacgga tgtccggtgt cgcggtattc gccattgcgg ctctcccttt
     1561 cagaagagaa gcgtcccggt ggggggacgt gacccgccag ccggtctatg caagcggtcg
     1621 aggcgcgtca acagttttcc gctgcatcgc agcaatcgac tgcgaattca gcgtgttgac
     1681 ggcctgcgcc ggccagaacg ggtccaggcc gctaacgtga ctgcggcaaa cgcggaacga
     1741 cgcgcgcgcc tacgcgttag atgccgaagc aagcaaacaa gcgccgcggc tcctggcaac
     1801 agttcggcgc gccggacctt ccgccgacgt ggttgtattc aggtggccgt gcttccgccg
     1861 ccatacaaac aggagcggtg gccatgctga aaggatcata tcaatgccgc gaaacccttc
     1921 ccgcaccggc acgaccattg tcctggccgc cttggtcgcg gccacctcgc cgacgctggc
     1981 gcagaacccg ccaggggcgt cgcccgccaa ccccaccgta ccggtggcgc cgccagcagc
     2041 ccctgcagcg cctgccgaga agatcgtgcc tccggacgga gggaccccca gcaaccggct
     2101 gtcccggcaa ggcggcgtga tcaacccgcc taacatcgat cccgggatga cggtcaaccc
     2161 gcccaaaagc ggcagcacca cgacgcctgt catcccgccg ccaggctcgc caggcggcaa
     2221 tccgtcagtt gtcccgaagt agggctctta gcgcggcgca aatcgtcgca atttcgagat
     2281 cggtcagctc cggatagacg ggcaaactaa gaatctccgt cgccgtacgt tccgtttccc
     2341 ggcacccgcc agggccgaga gcgactcggc cggcatacgc tggttgcaga tggacgggga
     2401 cgggatagtg gattcccgta cccacaccgt ccgctttgag cctcgcctgc agccggtcgc
     2461 ggtgggcgga tcgcgtcacg taaagatgga acacatggct gacgccgtcg ctttgccttg
     2521 ggggcagcag cgtcccgccg gccaaggcgg cggtataggc cgcggcgatc tccgcacgcc
     2581 gggcgttctg ccggtcgagg tggggcaact tgacccgtag gatggccgct tgaatctcat
     2641 caaggcggct gttcacgccg acctcattgc tgatgtaatg cttgtgccag ccatattgcc
     2701 gcagcgccgc gacacgctct gccagattcg ggtcggacac cgcgaccgca ccgccatcgc
     2761 caagcgcacc gagatttttg gtgggataga agctgaaggt ggcggcatcg ccaaaggtgc
     2821 cgactcgctg gccatgcagg cgcgcgccgt gggcttgggc acagtcttct atcagcgcga
     2881 cgccatgctt gcggcaaagc gggacgattt ggtcgagatc ggccggttgg ccataaagat
     2941 gaaccggtat aacagctttt atcggtgcca ggtccggcgg cggattttcc agcacgaaac
     3001 gcagcgcgcc cgggtccata gtgtagcgag ccggatcgat gtcgatcaac aacggggtcg
     3061 cgccggtcat ttctatagcc gcgaccgtcg ccacggcggt gtgggagaca gttaccacgg
     3121 agcaacccga accaatgtcc agggcgcgca aggcaagcgc gatggcgtcg gtgccgttgc
     3181 cgcacccgat cacggaagac acctcaagcc aggcggcgaa ttccgcttcg aaggcgcgga
     3241 gttcgttgcc gaggatgtac cagccggagg acagggcgca ggcgacggcc tcatcgattt
     3301 cggacttcaa tgcgcggtat ccggcgccgg ggttcgcttg cggaatcata ggtaatccgc
     3361 caaccgcgtc ctgtaccaat ccagcgtgcg gccgatacca tcctgaaggg aagtttcggg
     3421 cttccagccg gatgcggcac gaaacgcgct gtcgtcggcg tggtagctac cgatgtcaat
     3481 acgggcccga tcggcgggaa actcgcgggt ggtgtagctt ccggtgccgc cggacaggcg
     3541 caccacgagg tcggccagtt cctgcagcga tgccgggggg gagccgccaa tgttgaaaat
     3601 gccaccgtga cacgaaggtt gttcggctgc cgccaggaaa gcatccgtca cgtcgtcaac
     3661 ataggtcagg tcgcgaagct gggcgccgcc ccacacctcg aacgggcgat gctccagcac
     3721 gcagcggatc cagataccca ggaaagtctg ccgcgcgtcg cggatgcgca gccgcggccc
     3781 gtagcagttg gtcaggcgga gagagaccac cggacggttg cgtacacggt tttccagcat
     3841 ccagtattgc tcgccggcga attttgaaac gccgttggcg tcgggcgggg cgaccggctg
     3901 gttttcatcg accggcagct tttcgacgcg gccgtaaaac tgccgggtcg aagcgtggac
     3961 gacgatggcg tccggcgcag cctcgcgggc cgcctggatc agccggacct gggcgacggc
     4021 gttgaccgcg atatcggcca gaggatccgc ctgcccgccc atatggctgg tctgcgcggc
     4081 caggttgaaa atcacctcgg caccttcgca aagcgggcgg agatcgagtt cccggatgtc
     4141 gccgcggacg aaatccacat ccgcgccgtc gaggttgcgc ggattggcac caccgccaaa
     4201 caggaatcca tccaatgccg tgaccctggc gccccgcgca gccaaagcat ggcataggtt
     4261 ggagccgagg aagccggcac cccccgtgac gaaaaccttt cggcctgacc agttcatgag
     4321 ccctccttac agcccggttc tgaagctaca aaatggccag aatacggttt gtagtcgtta
     4381 tgtttaaggc gaaaccgcgt ctatgcggtc ggcatcgccc ggcgccacag gctcgcgtgt
     4441 ggccccgtca acaggctggc gacccacaat ccaccggcgg tcagcacgat cgccggccct
     4501 gccggaatgt ccaggttaaa ggatagaatt aggccaccgg ccgacgagcc acaggccagc
     4561 agcaccgcga cgcgcacctg acccgccaga gaatcggacc aatgtcttgc cgagatagcc
     4621 ggcaacatca tcaggccaac cgacatcagg gtgccaagcg cgacaaagcc cgacacgacg
     4681 cagagcacga ccagcatcag gaaaatcaag tgccaaatcc ccccgctcct ggttgtggcc
     4741 gccatgaaac cggggtcca
//
LOCUS       DASWQN010000031         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_927272, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000031 DASWQN010000000
VERSION     DASWQN010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927272"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VGC82_00540"
     CDS             <1..762
                     /locus_tag="VGC82_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421793.1"
                     /translation="RVHDPKLAELLTPRGYFIGERRIIAENGYLEIFNRDDVSLIDVK
                     SNPIMEITPSGLKTRNKEYPLDILIFATGFDSGTGAMMKVDLRGKSGQRFRNKWADGP
                     CTYLGLMANGFPNMFMIAQPGSPSIRSQVLVSIEQHVDWIFEMLEHARQLDVVEIEAS
                     RAAEDAWTQHVAAVSAKSLFARAETQYVGANIPGKPRVYLAYLGGVGVYRKTCDTVRE
                     NGYEGFILKTMTGVLPGSKVWSGATETPLVWGSAV"
     gene            complement(936..>2405)
                     /locus_tag="VGC82_00545"
     CDS             complement(936..>2405)
                     /locus_tag="VGC82_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0421794.1"
                     /translation="ALSSVDTVFNTSLVTTNHGFAANDTLFGINAKREISGQLAEGYA
                     IEDDGKTYVIRLREGLKFHNGEPVRAQDAVQSLKRWAGRETFGQTLAKFVNEWGVKDD
                     LTIRITLTRPVPIFLEAITRGSASIPFIIPEHIAKTDPFTQITDPTGCGPYKFVREEF
                     VAGSFAAYARNPDYKPAKGTPDWTSGAKIAHFERLEWIAMPEQATAAAALRSGEIDWY
                     EQVQPDLIAQLKNSGSIVVGTANPTNFNGILRFNHLHPPFNNPAVRRAVLMAVNQKDY
                     MEALTGGDSVVDPNCKSMFPCGTTYGRQVGGGAMGGDLDKARAMLKASGYDGAKAVVL
                     SPADVPTIHPMGEVTFDLLQKLGMNAEFAAMDWATLVGRRASKEPVDKGGWSIFHTWA
                     PSSILGTPVEHFPMRGLGQKGWAGWFDDQRIEELTAEWTVATSEEARSAAIDELQKRG
                     FDLVPFILCGQFPIRTAYRNYLTGMIEGGAAYMWNIRRI"
BASE COUNT          477 a          678 c          725 g          525 t
ORIGIN      
        1 cgcgtgcatg atccaaaact ggccgagctg ttaacgccga gaggctactt catcggcgaa
       61 cggcgaataa tcgccgagaa cggatatctt gagatcttca accgagacga cgtcagtctc
      121 attgatgtca agagcaatcc gatcatggag attacgccgt ctgggttgaa gacacgcaat
      181 aaagaatatc cattggacat tctgatcttt gcgaccggtt tcgacagcgg aaccggcgca
      241 atgatgaagg tggacttacg aggcaagagc ggtcagcggt ttcgcaataa gtgggccgat
      301 gggccatgca cctatctagg tctcatggcc aatggattcc caaacatgtt tatgatcgct
      361 cagcctggta gtccgtctat tcgttcgcag gtcctggttt cgattgagca gcacgtcgac
      421 tggatttttg agatgctgga gcatgcgcgt caactcgatg tcgtcgagat tgaggccagc
      481 cgagcagccg aggacgcatg gacgcaacac gtcgcagccg tgtcggcaaa gtcgttgttt
      541 gcgcgtgctg aaacgcaata tgttggtgcg aatatccccg gcaagccgcg cgtctatctt
      601 gcctatctcg gtggtgtcgg ggtctatcgg aagacctgcg acactgtgcg cgagaacggt
      661 tatgagggat tcattctcaa gacgatgacc ggagttctac ccggaagcaa ggtatggtca
      721 ggcgcaaccg aaacaccctt ggtgtggggt agcgcggtat aggctggtgg gattcacgcg
      781 atgcgtggcc gcggcaagat ccgcccgcgc gtcagcattg aggagcgcat cgttcttgtc
      841 gccgcgccgg caggttcacg cttcaagccg atggggctgg ggggatcgcc gctgttatgg
      901 ggcacttcgg tagcacctcc aggttctgga cgggttcata tccgccttat gttccacatg
      961 taggccgcgc cgccctcgat cataccggtc aggtagttcc ggtaggcagt tcggatcggg
     1021 aactgtccgc acagaataaa cggcaccagg tcgaagccgc gcttctgcaa ttcgtcaatt
     1081 gcggcgctgc gcgcctcttc cgaggtggcc acggtccact cggcggtcaa ttcctcgatc
     1141 cgttggtcgt cgaaccaacc ggcccagcct ttttgaccga gaccgcgcat cgggaaatgc
     1201 tccactggcg taccgaggat ggaagacgga gcccaggtgt ggaagatcga ccagccgcct
     1261 ttgtcgaccg gttccttgct ggcgcggcgg ccgaccaggg tggcccagtc catcgcggcg
     1321 aattcagcgt tcatgccgag cttttgcaac aggtcgaacg tcacttcacc catcggatga
     1381 atggtcggga catcagccgg acttaagacc actgctttgg cgccgtcata tcccgatgcc
     1441 ttcagcatcg cccgtgcctt gtccagatca ccacccatgg cgccaccgcc gacctgccgg
     1501 ccatacgtcg taccgcaggg aaacatggat ttgcagttcg gatcgactac ggaatcgccg
     1561 ccggtcaggg cttccatgta gtccttctgg ttcaccgcca ttaacacagc gcggcggacc
     1621 gccgggttat tgaacggcgg atgcagatgg ttaaaccgca ggatgccgtt gaagttcgtg
     1681 ggatttgcgg tgccgacgac aatgctcccg ctgtttttca gttgcgcgat cagatcgggc
     1741 tgcacctgtt cgtaccagtc gatctcacca gaccgcagcg cggcggcagc ggtggcctgt
     1801 tccggcatgg cgatccattc cagtcgttcg aaatgcgcga tcttcgcacc ggaagtccaa
     1861 tccggtgtgc ctttcgccgg cttgtaatcc gggttgcggg cataagcggc aaagctgccg
     1921 gcgacgaatt cctccctgac gaacttatac ggcccgcagc cggtggggtc cgtgatctgc
     1981 gtgaaaggat cggtcttggc gatatgttct ggaatgatga aagggatcga cgcgctgcct
     2041 cgggtgattg cctccaggaa aataggcacc ggacgagtca gtgtgatgcg gatcgtgagg
     2101 tcatccttca cgccccattc gttgacgaac tttgccaacg tctgaccaaa ggtttcacgg
     2161 cctgcccatc gtttcaggct ttgcaccgcg tcctgcgcgc gcaccggttc gccgttgtga
     2221 aatttcaaac cttcccgcag cctgatgaca taggtcttac cgtcatcctc gatcgcgtag
     2281 ccttcggcga gctggcctga aatctcgcgt tttgcattga tgccgaacag ggtgtcgttg
     2341 gctgcgaaac catgattggt ggtgaccagg gaggtgttga aaaccgtgtc gaccgaggac
     2401 agcgc
//
LOCUS       DASWQN010000032         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2510804, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000032 DASWQN010000000
VERSION     DASWQN010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2510804"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VGC82_00550"
     CDS             <1..1167
                     /locus_tag="VGC82_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEY0421795.1"
                     /translation="IPTDIWNEEIGEIDYTPVGAPKYGPDPAEIRKAAAAILAAKRPV
                     IYAGTGVHWAEAWPQLREFAELLGAPVTTSLGGKSAFPENHPLSLGSGGNAVPKPVHH
                     FVANSDLIIGIGCSFTETNFGIKFPAGKKFIHATLDPNHLNKDVISAIGLVGDAGLTL
                     SALIGELRQTIKANRDPSAVVAEITTVREEWMAQWRPLLNSNEAPLSPYRVIWELMNT
                     VDPKNTIITHDAGSPRDQISPFWVSTEPLSYIGWGKTTQLGMGLGLAMGAKLAKPDKL
                     CINLWGDAAIGFTGMDFETCVRERIPILSILLNNFSMAIELKVMPVSTEKYRSTDISG
                     DYAAMARAFGGYGERVTQPGDIKAAIQRGIEQTQAGKPALLEFLTAKETRVSKF"
BASE COUNT          290 a          467 c          481 g          276 t
ORIGIN      
        1 atcccgaccg acatctggaa cgaggaaatc ggcgagatcg actatacgcc ggtcggtgca
       61 ccgaaatacg gtcccgatcc ggccgagatc cgcaaggccg ccgccgccat cctggccgcc
      121 aagcgtccgg tcatttatgc cggcaccggc gtccactggg cggaagcctg gccgcaactg
      181 cgcgaattcg ccgagctgct cggcgcgcct gtcaccacca gcctcggcgg caagagcgcc
      241 ttccccgaga accatcccct ttcactcggt tcgggcggca acgccgtgcc gaagccggtg
      301 caccacttcg tcgcgaattc cgaccttatc atcggcatcg gatgctcgtt taccgaaacg
      361 aatttcggca tcaagttccc tgccggcaag aagttcatcc atgccacgct ggatccgaac
      421 cacctgaaca aggacgtgat cagcgccatc ggtctggtgg gcgatgccgg actgacgttg
      481 tcggccttga tcggcgaact gcgccagacc atcaaggcga accgcgatcc gtccgccgtg
      541 gtggccgaga tcaccaccgt ccgcgaggaa tggatggcgc agtggcggcc gctgctgaat
      601 tccaacgagg ccccgcttag tccctatcgg gtcatctggg agctgatgaa cacggtcgat
      661 ccgaaaaaca ccatcatcac ccacgatgcg ggcagtccgc gcgatcagat ttcgccgttc
      721 tgggtgtcca ccgaaccgtt atcctatatc ggctggggca agaccaccca gcttggaatg
      781 gggctggggc tggccatggg cgccaagctg gcgaagcccg acaagctgtg catcaacctg
      841 tggggcgatg cggccatcgg cttcaccggc atggacttcg aaacctgcgt tcgcgagcgg
      901 atccccatcc tgtcgatcct gctgaacaac ttctccatgg cgatcgagtt gaaggtgatg
      961 ccggtttcca ccgagaaata ccggtccacc gatatctccg gcgactatgc ggcgatggct
     1021 cgggcgttcg gcggctatgg cgagcgggtg acccagccgg gggatatcaa ggcggcgatc
     1081 cagcgcggca tcgagcagac acaggctggc aaaccggcgc tgctagagtt ccttaccgcg
     1141 aaggaaacgc gggtttcgaa gttctgaggc cagagtcggg cagcgttgcg ggcgggcgct
     1201 gcccgaactc gggcatgtgg agaaagggtt tcgggttccg gcgcaccttg atgcgaggta
     1261 gatttcgggg attttgacgc tggcggatcg tggggccgcg ccgctaggcc cgtggttggg
     1321 tctgaccgtg gcttttaaca cggagatcac cgcgggcact gaggtcacgg agaagattag
     1381 acagcttcgc cgggatattg gaactcgtgg ttgctggaca acggggagcc acgacggaaa
     1441 gttcccgacg atctctgctt ccttggcgcg ctctgcactc tattacctgt ggcacgcacg
     1501 agcggcgcca gatt
//
LOCUS       DASWQN010000033         4310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_531434, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000033 DASWQN010000000
VERSION     DASWQN010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531434"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VGC82_00555"
     CDS             complement(<1..602)
                     /locus_tag="VGC82_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0421796.1"
                     /translation="MSWQRVAQSMALVLALFAAACSPQPASARSAPESLSPLVKKVLP
                     AVVNIAVTETVSGGDMLSELPPELRDTPLGREFRRRFGNRRSQVAGAGSGFIIDPSGI
                     IVTNNHVVDHADKIVVSLTDGRQLPARVLGRDELTDVAVIKVQTNEALPSLTWGDSRR
                     AEVGDWILAAGNPFGLGGSVSVGIISAQGRDLGSGPFDNFL"
     gene            complement(723..3158)
                     /gene="hrpB"
                     /locus_tag="VGC82_00560"
     CDS             complement(723..3158)
                     /gene="hrpB"
                     /locus_tag="VGC82_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEY0421797.1"
                     /translation="MAPDFALPVADALPALQSALNAGRNAVLVAPPGAGKTTLVPLAL
                     LDAPWRGDGRILMLEPRRLATRAAATRMAFLIGQPVGQTIGYRTRLDGAGSAETRVEV
                     ITEGLLLRRLQSDPGLDGVVAVILDEIHERSLESDLGLALCLDLQRQLRPDLRLLAMS
                     ATADGARLSGLMNAEIVESAGRMFPVTVEHQKRDIPHIRDLSDAVARAIRSALADHVG
                     DILAFLPGMAEIRRTQSALGGCGALVLPVHGDLPPGEQDRALQPAETRRVVLATSIAE
                     TSLTVPGVRIVVDGGWRRTPRLDAATGLTRLTTARISRAAADQRAGRAGREAPGIAIR
                     VWSPALHRGLPAFDRPEILEAELSGLVLECAAWGTPPADLSFQDKPPAGALAAAAALL
                     QDLGALDPTDRITNTGQQMARIGAHPRLAAMMLAAETHEQAALAADLAALLEERDPLR
                     SQDAPADIATRIAAIANGAPDADRGALHRIRQTADQYRRRLRIPRNTEAAGEPGPLLA
                     AAFPDRIAQRRGEPGSFRLSGGGSARLPITDPLSKAGLLVAASLELKAASRIRLAAVL
                     DPDALPPSLAARVTEQVEAGFDPVTGTVLARRRRRLGTLILSDRTEPGDPVRITEALA
                     NAIAAQDLRPLPWSEATRQLQARVAMMRQIEPAGDWPDIGDKALNASVQDWLTPYLAG
                     MTRLSDVQKLDLHAILHGMMSRDLAARLDRDLPSHLSLPTGRAAIDYNEAPPLASAKA
                     QAFYGMTATPALAGGRVPLRLALLSPAGRPIAVTADLGGFWKGAWADARRDMRGRYPR
                     HHWPEDGAAPVEP"
     gene            3581..>4310
                     /locus_tag="VGC82_00565"
     CDS             3581..>4310
                     /locus_tag="VGC82_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0421798.1"
                     /translation="MSFTTQANTPNNLLASLPPQDRAHLASISRLAHPLQGQVLTSRS
                     DTGNDVWFPHTGIVALTAIDADGRSVQTGLVGRDGCVGLEALFDRVPTLPDAIVQIDG
                     PMSVIPATQLRFAVRDRLSIQVPMSRFLYGLAAQSLQTIACNRLHSLLARCCRWLLTM
                     RDEAGSDDLPLTQEALATLLGGGRPRINGLLATLETNGWVQRRRGWIHVLAKSELEAH
                     SCDCYRIVRHTCASLRLGAARNPTV"
BASE COUNT          739 a         1346 c         1520 g          705 t
ORIGIN      
        1 aggaaattgt cgaacgggcc ggagccaagg tcgcgcccct gcgccgagat gatgccgacg
       61 gaaacggaac cgcccagccc aaacggattg ccggccgcga ggatccagtc tccgacctcg
      121 gccctgcgcg agtcgcccca ggtgaggctg ggcagcgcct cattcgtttg caccttgatg
      181 acggcgacat cggtcaactc gtcgcggcca aggaccctag ccggcagttg gcggccatcg
      241 gtcagggaga cgacgatctt gtccgcgtga tccaccacat gattgttggt cacgatgatc
      301 cctgaggggt cgataatgaa gccggatccg gcgccggcca cttgcgagcg gcggttgccg
      361 aaacgccggc gaaattcgcg gccgaggggc gtgtcgcgca gttcgggagg cagttcgctg
      421 agcatgtcgc cgcccgacac cgtctcggtg acggcgatgt tcaccaccgc cggcaggact
      481 ttcttgacca gcggcgacag gctttccggt gcggagcggg cgctggctgg ttgcggcgag
      541 cacgccgcgg cgaacagcgc gaggacaagg gccatgcttt gggcgacccg ctgccaggac
      601 atggcgttcg gccgaaacga tgctggggcg gctggcatgg tgaggacggg ttctccaacg
      661 caacactttg tcaaatggca tggaaccaag gctgtaacaa gaaaccccgg atcggttttt
      721 gatcagggtt ccaccggagc ggcgccatcc tccggccaat ggtgtctggg gtagcggccc
      781 cgcatgtcgc ggcgggcgtc agcccaggcg cctttccaaa atccgccaag gtccgcggta
      841 acggcgatcg ggcgtccggc gggggacaac aacgccagcc ggagcggaac gcgcccgccc
      901 gccagtgccg gtgtggccgt catgccatag aacgcctggg cctttgccga ggccagcggc
      961 ggtgcctcgt tatagtcaat tgccgctcgg ccggtcggaa gcgacagatg ggacggcagg
     1021 tcgcggtcaa ggcgggcggc gagatcgcgg gacatcatac cgtgcaagat cgcgtgcagg
     1081 tccagctttt gcacgtccga caagcgggtc atgccggcaa gatagggcgt cagccaatcc
     1141 tggaccgagg cgttcagcgc tttgtcgccg atgtccggcc aatcgccggc gggttcgatc
     1201 tgccgcatca tcgcaaccct ggcctggagt tgccgcgttg cctcggacca gggcagcggg
     1261 cgaagatcct gtgcggcaat ggcgttcgcc agcgcctcgg tgatccgaac agggtcgccc
     1321 ggttccgttc ggtcggacag gatcagcgtg cccagacgcc ggcggcggcg tgccagcacc
     1381 gtgccggtga ccggatcgaa gccggcctcg acctgttctg ttactcgggc ggcgagcgac
     1441 ggcggcagcg catcggggtc gagcacggcc gcgagccgga tgcgcgaggc ggctttcagt
     1501 tccagcgacg cggcgaccaa caggccggcc ttggacagcg ggtcggtgat gggcagccgt
     1561 gcgctgccgc cgccggagag tcggaaagag cccggttcgc cgcggcgctg ggcgatgcgg
     1621 tcagggaacg cggcggcgag cagcggtccc ggctccccgg cggcctcggt gtttctggga
     1681 atgcgtaggc gacggcggta ttgatcggcg gtttggcgga tgcggtgcag cgcgccccgg
     1741 tccgcgtcgg gcgcgccgtt ggcgatggct gcaatgcggg tggcgatgtc ggccggagcg
     1801 tcctgcgaac gcagcgggtc acgctcttcc agcaacgctg cgagatcggc ggccagggcg
     1861 gcctgctcat gggtttcggc cgccagcatc atcgccgcca gacgcggatg ggcgccaatc
     1921 cgagccattt gctggccggt gttggtgatg cggtcagttg ggtcgagcgc gccgaggtcc
     1981 tgcagcaatg ctgcggcggc ggcaagagcg ccggccggcg gcttgtcctg gaacgacagg
     2041 tctgccggag gtgttcccca cgcagcgcat tcaagcacca ggccagagag ttccgcctcc
     2101 aggatttccg gacggtcgaa ggccggaagg cctcgatgca gcgcgggcga ccacacacgg
     2161 atcgcgatgc ccggcgcctc gcggccggcc cggccggcgc gctggtcggc ggcagcgcgg
     2221 gagatgcggg cagtggtcag ccgggtcaaa ccggtcgccg cgtcaagcct gggcgttcgc
     2281 cgccagccgc cgtcaacgac gatgcggacg ccgggaacgg tcagcgacgt ttccgcgatg
     2341 gacgtggcga ggacgacgcg gcgggtttcg gcgggctgca acgcgcgatc ctgttccccc
     2401 ggcggcaggt ccccgtgcac cggcaggaca agggcgccgc atccgcccaa agcggattgg
     2461 gtccggcgaa tttcggccat gccgggcagg aaggcaagga tatcgcccac atggtcggcc
     2521 aacgcggatc ggatggcgcg ggcgacggcg tccgacaggt cgcggatgtg cgggatgtcg
     2581 cgtttctgat gctcgacggt caccgggaac atgcgaccgg cgctttcgac gatctcggcg
     2641 ttcatcaggc cggacaggcg ggcgccatcg gcggtggcgg acatcgccag caggcgcaga
     2701 tcgggacgca gttgccgctg caggtcaagg cacagcgcca ggccgaggtc ggattccagc
     2761 gagcgttcgt ggatttcatc caggatgacc gcgacgacac cgtcgaggcc ggggtcggat
     2821 tgcagccggc ggagcagcag gccttcggtg atcacctcga cccgggtttc ggctgatccg
     2881 gcgccgtcga ggcgggtgcg gtagccgatg gtctgcccaa cgggttggcc gatcaggaac
     2941 gccatgcggg tcgcggcggc gcgggtggcc aagcggcggg gttccagcat caggatccgg
     3001 ccgtcgcccc gccagggcgc gtcgagtagg gcgagtggga cgagcgtggt cttgccggca
     3061 cccggcgggg cgacgagcac cgcattgcgg ccggcgttca gcgcagactg cagcgcggga
     3121 agagcatcgg cgacgggaag cgcaaaatca ggagccataa ggaccggttt aagccgcccg
     3181 gcatgccgtg gctattcagg cggggccgca accagatcga ggcgccgccc gatccgcacg
     3241 atttgcgggt cgatccggtc cacgcggcgg ttaagggaca gcgggcaatc acgctctaaa
     3301 tcctccaaat aaggattcat ttcgctggtc gggtgcgaag gatcttcgaa tgtgccgcat
     3361 cgcggcgaaa aacttgctgg cagcctgtcg gattaagact gtcacacaac acgggctttc
     3421 ttccacggcc gatcgaacag tccgcacctc tgtcatgtcg cctatgcgct aattggataa
     3481 ttccgcactt ttcccgcaga taccaaccgg ctatgcatca tgtccggttc ttgaagcact
     3541 ttacgagcga accgacagct gcaaggtgcg ttcgttcgcc atgtcgttta caacacaagc
     3601 caacacgccg aacaatctgc ttgccagtct tccgccgcag gatagggccc atctggcatc
     3661 gatcagccgt ctcgcgcatc cactacaagg ccaggttctg actagccgtt ccgacaccgg
     3721 taacgatgtg tggttcccgc ataccggaat agtcgcgctg accgcgatcg acgccgacgg
     3781 acgaagcgtg cagaccggat tggttggccg tgacggatgt gtcggattgg aagcgctatt
     3841 tgatcgcgtg ccgacgttgc ccgacgcaat cgtgcagatc gacggaccga tgtcggtcat
     3901 tcccgcaacg caactgcgat tcgcggttcg cgaccggctg tctatccagg tcccgatgtc
     3961 acggtttctc tacggtttgg cggctcagtc gttacagacg atcgcctgca accggctgca
     4021 tagcctgctc gcccgctgtt gccgctggct gctgacgatg cgcgacgagg ctggaagcga
     4081 cgatctgccg ttaacgcaag aagcactcgc gacgctgctc ggaggcggca ggccccgcat
     4141 caacggtctg ctggcgacat tggaaaccaa cggctgggtg caacgccgac gcgggtggat
     4201 ccatgtgttg gcgaagtcag agctggaagc acattcgtgc gactgctatc gcatcgttcg
     4261 tcacacctgt gcatcactac gattaggtgc cgcgcgaaat cccactgtca
//
LOCUS       DASWQN010000034         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1191307, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000034 DASWQN010000000
VERSION     DASWQN010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191307"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..500
                     /locus_tag="VGC82_00570"
     CDS             78..500
                     /locus_tag="VGC82_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421799.1"
                     /translation="MRATNLLKVAAEAEILRIQFMLKRQGVRAAFGLVALIFVLGVLV
                     LANVAAWQVLRLYIEPIYATLVMLGVNLVIAVIFGILAARSSPSSTERDALQVRQRAL
                     REARSSLALGALVPVAGVLLRSRRNNAAKKVPLWRRLS"
     gene            complement(493..1533)
                     /locus_tag="VGC82_00575"
     CDS             complement(493..1533)
                     /locus_tag="VGC82_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY0421800.1"
                     /translation="MASHDIIVIGGSTGSIPVLRQLCSDLPVDLPAAVFVVVHVGAQG
                     QDLLAGILNGSGPLPVATAQDGEAIRRGRIYVAPGDRHLLVGDDVIRLGRGPRENMTR
                     PAIDPLFRSAGVNYGPRVIGVVLSGNLNDGSAGLAAIKRCGGLAVVQSPSEAEANEMP
                     CGALQSCDVDYQARVSDLGSLLVHLAAEQAGPAVPVPPGIALEVSFALGRNSDSHTLR
                     QIAAPSTIPCPACGGVLSEVHDKDALRFRCQIGHAYTAECLDREQESSTHDALGIALR
                     TLEARATLLERMAEDARDKGRAQSASDFAARGNEYRVHAGTIQQAMLNLPPGGQGGGG
                     QSGGGQGGRRSN"
BASE COUNT          365 a          639 c          645 g          362 t
ORIGIN      
        1 cgcgttcgaa cagccgttga ttacgttgct gctgtcttgt caggccgggt acttggccgc
       61 tcgtttagga cgccgccatg cgcgccacta atttactgaa agtagccgcc gaggcggaga
      121 tcctgcgcat tcagtttatg ctgaaacggc agggcgtgcg tgcggcgttt ggtcttgtcg
      181 cgcttatttt tgttctgggc gtgctcgtcc tggcaaatgt cgcggcatgg caagtcttgc
      241 gactgtatat cgagccaatc tacgcgacgc tggtcatgct cggtgtcaat cttgtgatcg
      301 cggtaatatt cggaatactg gcggccagat cttcgccgag cagtaccgaa cgcgatgcgc
      361 ttcaggtacg acagcgggcc ctgcgggagg cgcgcagttc gcttgcgttg ggtgcgctcg
      421 tgccggttgc cggcgtcttg cttcggtccc gccgtaacaa tgccgccaag aaggtaccgc
      481 tctggcggcg gcttagttag accgccgccc gccttggccg cccccgcttt ggccaccccc
      541 gccttggccg ccaggcggca ggttcagcat ggcctgctgg atcgtcccgg cgtggacgcg
      601 gtactcgttg cctcgggctg cgaagtcgga tgcggattgg gcgcggccct tatcgcgggc
      661 gtcctctgcc atccgttcca gcagggtggc gcgcgcctcc aaagtacgta gcgcaatccc
      721 taacgcgtcg tgggtcgaac tttcctgctc ccgatcaaga cattcggcgg tgtaggcgtg
      781 gccgatctgg cagcggaacc gtagcgcatc cttgtcgtgc acctccgaca gaacgccgcc
      841 gcaagcggga cagggtatcg tgctcggtgc cgcgatttgc cgcagtgtat gcgaatcgct
      901 gttgcgtccg agtgcgaagc tcacctccag agcaatgccg ggcggcacag ggaccgcggg
      961 ccccgcttgt tccgccgcca ggtggaccag cagcgatccc agatcggaaa cccgcgcctg
     1021 atagtccacg tcgcaggact gaagcgcacc gcacggcatc tcgtttgcct ccgcctccga
     1081 tgggctttgc acgaccgcga ggccgccaca acgctttatc gccgccagcc cagccgaacc
     1141 atcgttcagg ttgccgctga ggacgacgcc gatcacacgc ggcccgtaat ttacgcccgc
     1201 agatcggaac aatggatcga tcgcgggccg cgtcatgttc tcgcgcgggc cccgccccaa
     1261 ccgaatgacg tcatccccga ccaggagatg acggtcccct ggcgcgacat agatccggcc
     1321 gcgccggatc gcctcgccgt cctgcgctgt cgcgaccggg agcggaccgc ttccgttcag
     1381 aatgccggcg aggaggtcct gaccctgcgc cccgacatgg acgaccacga aaaccgcggc
     1441 tggcaggtca acgggcaagt cggaacaaag ctgcctcagg acagggatcg agcccgtgga
     1501 cccgccgatg acgatgatgt cgtgcgaagc catgacgggt cccttgaaaa atccgtggac
     1561 aacaacggcg catttccaac tgccacgcaa gacggcgcct gcacaagtct ggcgaacctg
     1621 ggttcggcca ggccgttaaa tgaagaactg aaagggacac aggacatgga cgagcaggct
     1681 gacgaaaaga acccctcgaa ggagcaagga ctgccgcaac gcggccccgc ccagcccgcg
     1741 ggaccagatg acccgcgcga gcaatccgtt ccgcggcata cggcaggggg tgcgccaact
     1801 gatgccgcac atcaggacgc gggcgatggt gcgttgacgg gctctgtgcc cgccgggttg
     1861 actgcaaagg aactgttgga cgggtcgcgg gatgtcggtc gagacgatcc cgcgaccgaa
     1921 taaaccgtgt tcggcacgac accgcgtcaa gcctgattca agtgtagaaa aagcgatggc
     1981 gcgaacgatg cgttggctgc tgtttctggt t
//
LOCUS       DASWQN010000035         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2378827, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000035 DASWQN010000000
VERSION     DASWQN010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2378827"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VGC82_00580"
     CDS             complement(<1..336)
                     /locus_tag="VGC82_00580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0421801.1"
                     /translation="MKICVVTPYFQTEEAWVRHAHDSVRTQTIPARHILVCDGSAPAQ
                     IASFQGTHILLGRNYQDYGNTPRLIGCFKAITEGADAIAFLDTDNWFQNDHLESLARF
                     CLENNLDACS"
     gene            582..1085
                     /gene="ruvC"
                     /locus_tag="VGC82_00585"
     CDS             582..1085
                     /gene="ruvC"
                     /locus_tag="VGC82_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY0421802.1"
                     /translation="MVRLLGIDPGLLFTGWGLIDVDGNRLKHIADGVIATNSTDSVPA
                     RLKLLHDGLMALLDLHRPAEAAVEETYVNRNGTATLKLGYARGVALLTPALAGIPVAE
                     YAAKTVKLAVVGTGGAEKAQVAMMVRRLLPGATLRRADAADALAVAICHAHHRSSRLA
                     WAMASTG"
     gene            complement(1095..1580)
                     /locus_tag="VGC82_00590"
     CDS             complement(1095..1580)
                     /locus_tag="VGC82_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY0421803.1"
                     /translation="MDALLPLAERIGARLKERGETIGIAESSTGGLLSAALLSIGGAS
                     VYFRGGGVIYTAYARKAFLEIPNPLPAPIERASTEPYALLLADTVRARLDSHWGLGET
                     GATGPTGNRYGDKAGHTCIAVTAPGFSKVITLETGSPDRIANMRAFGVRALELLAEAL
                     G"
BASE COUNT          392 a          628 c          698 g          377 t
ORIGIN      
        1 cgaacacgcg tcgaggttat tttccagaca aaatcgcgca aggctttcga ggtggtcgtt
       61 ctgaaaccag ttgtcggtgt ccaggaacgc gatcgcgtcg gcgccctcgg tgatcgcttt
      121 gaagcacccg atcagtcgcg gcgtgttgcc ataatcctgg tagttgcgcc ccagcaggat
      181 atgcgtcccc tggaatgagg ctatctgggc aggcgcggat ccgtcgcaaa caaggatgtg
      241 cctcgccggg atggtctgcg tgcggacgct gtcatgggcg tggcgcaccc aggcttcctc
      301 ggtctgaaaa tacggcgtta caacgcaaat cttcatggcg gctactcgtc cggctggcaa
      361 tttcttcctt ggccgtaatg agacaatgac gcacccattg gccggagcga cacttagacc
      421 atgttcgtgc tgcgtagtga gcccgaacat ggtctaatgc cacacggcgt tgacgccgaa
      481 tcgaatctgc gcagggttgg tgtggcggat gcgtgaaaga tcaaccagtt accagccggc
      541 cgacccccgg tgaacaagcc gcgggagaat tcgatttggc catggtcagg ctgttgggca
      601 ttgatccagg actgcttttc accggctggg gcctgatcga cgtggacggc aatcgcctga
      661 aacatatcgc cgacggggtg atcgccacca acagcaccga ctcggttccg gcccggctga
      721 agctgttgca cgacgggctg atggcgctgc tcgacctgca ccgcccggcc gaggccgcgg
      781 tcgaggaaac ctacgtcaac cgcaacggca ccgcgacctt gaagttgggc tatgcaaggg
      841 gcgtagcgtt gctgacgccg gcactggcgg gaataccggt cgcggaatac gccgccaaga
      901 cggtgaaact cgcagtggtc ggcaccggtg gggcggagaa ggcccaggtc gcgatgatgg
      961 tacgacggct gcttccaggc gccacgctcc gccgagcaga tgcggcggac gcgctggcgg
     1021 ttgcaatttg ccacgcgcac catcgttcgt cccgcctggc ctgggccatg gcatcgacgg
     1081 gttgacgaga agaactatcc cagcgcctcg gccagcaact ccagcgcccg aacgccgaat
     1141 gcccgcatgt tggcgatgcg gtcgggactg ccggtttcca gggtgatcac cttggaaaag
     1201 ccgggcgccg tgacggctat gcaggtgtgg cccgctttat cgccgtaccg gttgccggtc
     1261 ggccccgtgg cgccggtttc acccagtccc caatgcgaat ccaagcgagc ccgaacggta
     1321 tcggcgagga gcagggcata aggctccgtg gacgcccgct cgatcggtgc cggcagcggg
     1381 ttggggatct ccaggaacgc cttgcgtgcg taagcggtgt agatcacgcc gccgccccgg
     1441 aaatagaccg aggcgccgcc gatcgacagc aaggcggcgg acagcaggcc gccggtggaa
     1501 gattcggcga tgccgatcgt ttccccccgt tccttcaggc gggcgccgat cctctcggcc
     1561 aggggcagca aggcatccat cgggtttccc tccgcttttg atggtggata ttcgcccgga
     1621 ctgcggtccg tgtcgatacg cagctgacgg attgaaggcg acggccgggt gactgcggct
     1681 cactccgtag tgattctgcg ggtggggaag aaaagtcgcg cggcgcgttt cctgctctgc
     1741 gggtccgact ccggtccgat ccacttgacg atgtgtaccg gccgatgcgt accggccacg
     1801 gagatctgag atgcaacggc taaaaaatac cgggaacgtg cctttgcctc gcccgacggg
     1861 acactggctg gcaggcatga tgatcgggtc gatcgtcatg accatggcca ctcatgcaat
     1921 gatactgggc gccggggcgg cgatcagcgg ctctacgttg aagaatcgat gatcctgtta
     1981 cagcgccgga tggaggtagc gaacacggcg cccgccgtgc gacaaggggc ccatgccgga
     2041 cgaccgtatc gacagtgcta atacgggaag cgataacgta gcggtcgccg cggag
//
LOCUS       DASWQN010000036         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_663384, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000036 DASWQN010000000
VERSION     DASWQN010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663384"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..954
                     /locus_tag="VGC82_00595"
     CDS             76..954
                     /locus_tag="VGC82_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0421804.1"
                     /translation="MTPLRQRMIEEMQIRNLAPHTQRTYVEQVARFARHSGRSPELLG
                     PDDIRAYQLYLVQEKRLSASSVVVAVSALRFLYNVVLKRGWDVDEVIPTCQTSQRLPV
                     VLSREEVSQLLAAVKDLHHRVILTICYAAGLRVSEVVRLKPTAIDSQRMVIRVEAGKG
                     RKDRYVMLSPKLLDLLRDYWRVVRPKEWLFPGDLPGQPISRFAVEEACRKARHQANIT
                     KPVTPHSLRHAFAVHLLEAGVDLRTIQLLLGHRSLSTTAHYLRLTTTKVCATASPLDA
                     LATVSRAMAAPVPVPA"
     gene            964..2166
                     /locus_tag="VGC82_00600"
     CDS             964..2166
                     /locus_tag="VGC82_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEY0421805.1"
                     /translation="MRPELEVADIFRRYGPAYREAHGDHLDGTARRVMAAIEACRTAT
                     LGGHVEQCTECGFVRYAYNSCRDRHCPKCQGLVRAEWLEARQTELLPVPYFHVVFTVP
                     APVADLAFFNKEVVYGILFHASAGALREVAGNRRYLGAEIGAIAVLHTWGQALHHHPH
                     VHCIVPGGGIAPDQTRWVSCQPGFFLPVRVLSRCFRDLFVQHLRTAYASGELRFPDAL
                     ADLADPAAFAARLDALARMEWVVFAKPPFGGPVQVLAYLGRYTHRVAIANSRLVRLAD
                     GQVSFTWKDYRHDGKTKVMTLEADEFIRRFLQHTLPDGFHRIRHIGFLANCHRAEKLA
                     LCRELLAAPPPAVPNEAPAPRRWQDRLRELTGQDVEVCPCCGGRMMTCGVLKPQPPPR
                     PPMWCDSS"
BASE COUNT          386 a          919 c          737 g          415 t
ORIGIN      
        1 gatcgcccgg cggcgccttc cgcgggacca tgtggttgcg ccctgcgcgc acttcattcc
       61 gcaaaggagc cgccaatgac gccgcttcgc cagcgcatga tcgaggaaat gcaaatccgc
      121 aacctcgcgc cgcatacaca acgcacatac gtcgagcagg tcgcccgatt tgcccgccac
      181 tccggtaggt cgcccgagct gctggggccg gatgatattc gcgcctacca gctctacctg
      241 gtccaggaaa agcgcctctc ggccagttcg gttgtggtcg cggtctccgc tctccgcttc
      301 ctctacaacg tcgttctcaa acggggctgg gacgtcgacg aggtcatccc cacctgccag
      361 acctcgcaac ggctgcccgt ggtgctgagc cgggaggagg tcagccagct cctcgccgca
      421 gtgaaggacc tgcaccatcg cgtcattctg accatctgct acgcagcggg tctgcgcgtc
      481 tccgaggtcg tccgcctgaa accaaccgcc atcgacagcc aacgcatggt cattcgcgtc
      541 gaagccggca aggggcggaa ggaccgctac gtcatgctgt cgccgaagct gctggacctc
      601 ctgcgcgact actggcgtgt ggtccggccg aaggaatggc tgttccctgg cgacctgccc
      661 ggacaaccga tctcgaggtt cgccgtggag gaggcctgcc gaaaggcgcg gcatcaggcc
      721 aacatcacca aacccgtcac gccgcactcg cttcgccatg cctttgccgt tcacctgctc
      781 gaggccggcg ttgatctgcg caccatccag ttgctgctcg gtcatcgcag cctcagcact
      841 acggcccact acctgcgcct gacgaccacc aaagtgtgcg ccaccgccag cccgctggat
      901 gccctggcaa cggtctcccg ggccatggcc gcccccgttc cggtgcccgc ctgagccgac
      961 gcgatgcgcc ccgagctgga ggtggcggac atcttccgcc gctacggacc ggcgtaccgc
     1021 gaggcccacg gcgaccatct tgatggcacc gcgcgccgcg tcatggcggc gatcgaggcc
     1081 tgccgcaccg ccaccttggg cgggcacgtc gagcaatgca ccgaatgcgg cttcgtccgc
     1141 tacgcctaca actcatgcag agaccgccac tgtccgaagt gccagggcct cgtgcgcgcc
     1201 gaatggctgg aggcgcgcca gaccgaactg ctgccggtgc cctacttcca tgtcgtgttc
     1261 accgtgccgg cgccggttgc cgacctcgcc ttcttcaaca aggaggtggt ctacggcatc
     1321 ctgttccacg cctcggccgg ggccctgcgc gaggtcgccg gcaaccgtcg ctaccttggc
     1381 gccgagatcg gcgcgatcgc cgtgctgcac acctggggac aggcactgca tcaccatccg
     1441 cacgttcact gcatcgtccc cggcggcgga atcgcgccgg accagacccg gtgggtttcc
     1501 tgccagccgg gtttcttcct gccggtgcgg gtcctgtccc gctgcttccg cgacctgttc
     1561 gtgcagcatc tccgcaccgc ctacgccagc ggcgagctgc gttttcccga cgcgctcgcc
     1621 gacctggccg atccagccgc cttcgctgcc cggctcgatg ccctggcgcg gatggagtgg
     1681 gtcgtctttg ccaagccacc gttcggcgga cccgtgcagg tgctcgccta tcttggccgc
     1741 tacacccacc gcgtcgccat cgccaacagt cgcttggtca ggcttgcgga cggccaggtg
     1801 agcttcacct ggaaagacta ccgccacgat ggcaaaacca aggtgatgac actggaggcc
     1861 gatgagttca tccgccgctt cctccagcac accctaccgg acggcttcca ccgcatccgc
     1921 catatcggct tcctcgctaa ctgccaccgc gccgagaagc tggcgctctg ccgtgagctg
     1981 ctcgccgcac cgccgccggc ggtgccgaac gaggccccgg caccacggcg ctggcaggat
     2041 cgcctgcgcg agctaaccgg ccaggacgtc gaggtctgcc cctgctgcgg cggtcggatg
     2101 atgacctgcg gggtcctcaa gccacaacca ccgccgcgcc ctccgatgtg gtgcgacagc
     2161 tcatgaccca gcgcccgtcc ttcctcatcc acccagtcct agcaggtaac ggcgatcgcg
     2221 gcgccgacca gcgcatgccc cgcctcacct gggccgtcgc cagcaggccc gcggagcttg
     2281 ccgacggcgc cgatctgcca ctctatgcgc cgtcagggat ccatggagcc tgtcagactt
     2341 ggaccagcct ccgtgtgaac ttcgcgaagc gtgcccatgc ggcgccccat cggacggcag
     2401 cagaatgcaa tccccatagc cgccgacggc ccgtggttta gtccaacccg ttttttg
//
LOCUS       DASWQN010000037         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2511072, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000037 DASWQN010000000
VERSION     DASWQN010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2511072"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..926)
                     /locus_tag="VGC82_00605"
     CDS             complement(171..926)
                     /locus_tag="VGC82_00605"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:NF002198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEY0421806.1"
                     /translation="MLIGSHVHQEDPLAAAAADGADVVQFFLGNPQSWKKPKPREDAE
                     VLKASSVPLYVHAPYLINVASANNRIRIPSRKILQDTCDAAAEINATAVIVHGGHADD
                     NDMEAGFQRWEKALAYLETDVQVYLENTAGGDHAMARYFDTIGRLWDHIGDSGIGFCL
                     DTCHAWAAGEGLIDAVERIKALTGRIDLVHCNDSRDAAGSGADRHANFGTGQIDPDLL
                     VAVVTAADAPVICETADAGRKDDIAFLREQTSH"
     gene            1015..>2331
                     /locus_tag="VGC82_00610"
     CDS             1015..>2331
                     /locus_tag="VGC82_00610"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEY0421807.1"
                     /translation="MLSVGLSVGRGIADITGEATECGMLGYGKIEQRTAGIHLRLRSR
                     AFVFQAGPQEANSRLLLVVAELPLPMQNVTDEVLRRLANSYGDTYCARNTLITTTHTH
                     SAPGGYCGHLLYNLSTSGFRPATFGAIVDGIVESVSYAHQDVAPAEVTLSHGELRDAN
                     INRSPSSFDRNPLADKDFFPGRIDPLTTLVRIDRGDRTVGAIHFFATHGTSMTNRNLL
                     ISGDNKGYAAYHWERIIGGADYLAGQPDFIAAFAQTNPGDMSPHVDGPIVGGPSASQD
                     ELDNTRRIGLSQFGDAVAQLNTGTPIGTGIDSRFTYVDLGAVQVRGEYTPDGQVHRTG
                     RALIAAATMAGTDEGEGFRGFRQGRNPFWDRLSDTIYRFAAALRAAQAPKGIVVPAHL
                     LNRLQPFVQEIVPVHLVRIGRLYLIGIPGEPTIVSGLRLRRTVASIV"
BASE COUNT          420 a          763 c          726 g          422 t
ORIGIN      
        1 ccggccggca ggtagatccg gaagctgcga tcgtgtccgc cgatgttgat gtggtggatg
       61 ctggtgccgg caacgaagcc cgtcggcagc atccaatcct gcaccgccga gcaacccgcg
      121 actaacagga ctacgccgaa cagggctgcg atgcggcgga tcacgcctgg tcagtggctg
      181 gtttgttcgc gcaggaaggc gatgtcgtcc ttacggcctg cgtcggcggt ctcgcagatc
      241 accggcgcat cggcggccgt gacgacggca acgagcaggt cggggtcgat ctgaccggtg
      301 ccgaagttgg cgtgccggtc ggcacccgaa ccggcggcgt cccgagaatc gttgcagtgc
      361 accaggtcga tgcgcccggt caacgccttg atccgctcga cagcgtcgat cagtccctcg
      421 cccgcggccc acgcatggca ggtgtccaga cagaagccga tcccgctgtc gccgatgtga
      481 tcccagagcc ggccgatggt gtcgaagtag cgggccatcg catgatcacc gcccgcggtg
      541 ttttccaggt agacctggac atcggtttcc aggtaggcca gcgccttttc ccatcgctgg
      601 aagccggcct ccatgtcgtt gtcgtcggcg tggccgccat gcacgatcac ggccgtcgcg
      661 ttgatttccg cggcggcgtc acaggtgtcc tgcaaaatct tgcgcgacgg gatcctgatg
      721 cggttgttgg ccgatgccac gttgatgagg tatggcgcgt ggacgtacag cggcacgctg
      781 gacgccttca acacctcggc gtcctcacgc ggcttgggtt tcttccaact ctgcgggtta
      841 cccaggaaga actggaccac gtcggcgccg tctgcggcag ccgcggccag aggatcttct
      901 tgatggacat gtgaaccaat aagcacccag ccagtctaat gaagactgcc agttcccatg
      961 cgggcctgcc gcatagggtg tgtgtcggta ggcaacaggt tggagggcga gccaatgctt
     1021 tctgtggggc tttcggtagg acgaggcatc gccgatatca ccggtgaagc gaccgaatgc
     1081 ggcatgctcg gctacggcaa gatcgagcaa cgcaccgccg ggatccacct ccggttacgg
     1141 tcacgcgcgt ttgttttcca agccggaccc caagaggcga actcgcgcct gctactggtg
     1201 gtcgccgagc tgccgctgcc catgcagaac gtgaccgacg aagtgctccg ccggctggcg
     1261 aattcgtacg gcgacaccta ctgcgcacgg aacaccttga tcacaactac ccacacccac
     1321 tcggcgcccg gcggttactg cggacacctg ctgtacaacc tgtcgacgag cgggtttcgg
     1381 cccgcgacat tcggggcgat cgtcgacggg atcgtcgagt cggtctccta cgcccaccag
     1441 gacgtggcgc cagccgaagt gacgctgtca cacggcgaat tgcgcgacgc gaacatcaat
     1501 cgctcgccct cgtccttcga tcggaacccg ttggcggaca aggatttctt ccctggccgc
     1561 atcgacccgc tcaccacgct ggtgcgcatc gatcgcggtg accggacggt gggtgcaatt
     1621 catttcttcg caactcacgg caccagcatg accaatcgca acctgctgat ctccggcgat
     1681 aacaagggtt acgcggccta ccactgggaa cgaatcatcg gcggcgcaga ctatctcgcc
     1741 ggtcagcccg acttcatcgc cgcgttcgcc cagaccaatc cgggcgacat gagcccccac
     1801 gtcgacggac ccatcgtcgg cgggccgtct gcttcacagg acgagctgga caacacccga
     1861 cgtatcggct tatcccaatt cggcgatgct gtcgcacaat tgaacaccgg cacacccatc
     1921 ggcaccggca tcgattcccg gttcacctac gtcgatctcg gtgctgtgca ggtccgtggc
     1981 gaatacacgc cggacggaca agtacaccgc acgggacgcg cgttgatcgc cgctgcgacc
     2041 atggcgggta ccgatgaagg ggaaggcttc cgcggctttc gtcagggccg aaacccattc
     2101 tgggacaggc tatccgacac catctaccgg ttcgctgcgg cgttgcgggc agcacaggcc
     2161 ccgaagggca tcgtggtgcc cgcccacctg ctgaaccggc tgcagccgtt tgtccaggag
     2221 atcgtgcccg tccacctggt gcgaatcgga cggctctatc tgattggcat cccgggagaa
     2281 ccgaccattg tctcgggact ccgactgcgg cgcacagtgg cctcgatcgt t
//
LOCUS       DASWQN010000038         5774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1587356, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000038 DASWQN010000000
VERSION     DASWQN010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587356"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..716)
                     /locus_tag="VGC82_00615"
     CDS             complement(63..716)
                     /locus_tag="VGC82_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0421808.1"
                     /translation="MHIDLNFLARLFPALLEGAEVTVELAVLTMAICIVWGLVVALGQ
                     ATRGPLGWLSGIYIQVVRNTPLLIQMYLVYFGLSLAGIPLSGFASGLLALCLQNGGYV
                     AEIYRGGLQSVSIRQWEAGRALGMRRWTLYRSVILPQVIARVIPPLANQGISITKDTS
                     QVAAIAVMEVMKVAQVWVESSANTYDVFFAVALIYLVLTSVISLGARVAERQLAFVS"
     gene            complement(720..1553)
                     /locus_tag="VGC82_00620"
     CDS             complement(720..1553)
                     /locus_tag="VGC82_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0421809.1"
                     /translation="MRRLQLLPAVLLILSAIAPARAQDSLLDTVLKRDKIIVATYSTS
                     PPLAYVDDKGDLVGFEIDMAHEIAKDLLGDPKKVEFVVVQSDGRFPAALSGKVDFGLC
                     STTIYPDRAVRIAFTRPYLDTGNSLVARKDAKIKTIQELNDPKYTYAILNVPPAIERA
                     KQLLPKVKTLLLDSPSALFLAVKTGRATAFNIDKPIADFYEHENEDLMRLDVAGTPFA
                     YVAQDAIFMKPGDFKWWLFLDTWVGELRYGSRYNKYQEWYQTWLHKDAPPQRGYDFNK
                     Y"
     gene            1965..5543
                     /locus_tag="VGC82_00625"
     CDS             1965..5543
                     /locus_tag="VGC82_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY0421810.1"
                     /translation="MWQFWIDRGGTFTDIVARDPEGKLSTHKLLSENPERYKDAAIAG
                     IRSILGLMPDAAIPPNLIESVKMGTTVATNALLERKGDRTLLLVNKGFADALRIGNQA
                     RPRLFDLAITLPTMLYERVAEIGGRVGMDGQEIEALDADLAREVCAKARADGIDACAI
                     VLMHAWKYPEHEQRLTDLAREAGFTQISASHAVSPLLRLVPRGDTTVVDAYLSPILRR
                     YVDQVASELSGTRLYFMQSNGGLTEAHSFQGKDAILSGPAGGIVGAARTAGMAGLDQI
                     IGFDMGGTSTDVALYAGSFERAFETAVAGVRMRAPMMAINTVAAGGGSILHFDGARMR
                     VGPDSAGANPGPACYRRGGPLTVTDANVCVGKIQPAHFPPIFGPHGDQPLDAEVVTGK
                     FAALAGEIGGDRTPRQVAEGFLRIAVANMANAIKQVSVQKGHDVSRFALQCFGGAGGQ
                     HACLVADALGMETVFIHPYAGVLSAYGMGLADQTTMKEQAVEIPLGAGTLAEIETLAE
                     KLATDAIGALEAQGANPADVTTAKSLHLRYAGTEAALIVPLRDLETIISDFTAAHRTR
                     FGFATPDRPVVVETVAAEATLPGSVATEATLPARETGEPAIIDRVRVFTGGTEIEAPV
                     LERTSLLAGDRIAGPALVREANATTVIEPGWTAEITALDHMILRRTEPLPSRVPAGSE
                     RPDPVLLELFNNLFMNVAEQTGAVLQNTSMSVNIKERLDFSCAIFDADGNLVANAPHV
                     PVHLGAMGESVRTVLEHRRGTLKPDDVVALNNPFNGGTHLPDITVITPVFDAGGTEIL
                     FFVGSRGHHADIGGITPGSTPPMSRTLEEEGVVIDDFLLVDGGHFRETEFRALLAGAR
                     YPARSPDVNVADVKAQVAANEKGVQELQRVVAQYGWPVVSAYMRHVMDNAEESVRRVI
                     ERLGSGRFDYKMDNGRDLCVSISVDRENRSATVDFTGTGPQHEGNFNSPPAVTRAAVL
                     YVFRCLVGDDIPLNDGCLKPLTLIIPPGTFLSPLPGAAVVAGNTEVSQATCNAIFGAL
                     GVIACSQATMNNFLFGDATRQYYETICGGTGAGPGFNGTSAIQTHMTNTRMTDPEILE
                     LRYPVRQEFFGIRHGSGGKGKWTGGDGSIRRIRFLEPMTAVIVSSRRKVAPFGLDGGS
                     DGAAGRQWVERADGSRETLTGTDSAELAPGDLFVIETPGGGGYGRA"
     gene            5583..>5774
                     /locus_tag="VGC82_00630"
     CDS             5583..>5774
                     /locus_tag="VGC82_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0421811.1"
                     /translation="MTNEIGYGVLTDAQWAELAPLIEQCRPPHKTAHDNLRRTIEAII
                     WRHDNGAKWRSIPSHLGPWW"
BASE COUNT         1100 a         1868 c         1775 g         1031 t
ORIGIN      
        1 cagcgtcatg cccgcgccgc ggaacaagta ccagtagctg tgcaaaagcg gggcaaaggt
       61 cttcaagaca cgaacgccag ttggcgctcg gcgacgcggg cacccaggct gatgacactc
      121 gtcagcacca ggtagatcag cgccaccgcg aagaacacgt cgtaggtatt cgccgaagac
      181 tccacccaga cctgtgccac cttcatcacc tccatgactg cgatggcggc cacttgcgag
      241 gtatccttgg tgatgctgat cccctggttc gccaacggcg ggatgaccct ggcgatcacc
      301 tgcggcaaaa tcacgcttcg gtacagcgtc caccgccgca ttcccagtgc ccgtcctgcc
      361 tcccactggc gaatgctgac cgactgcagt ccgccgcggt aaatctcggc cacgtacccg
      421 ccattctgca ggcaaagcgc cagcaagccg gaggcaaagc ccgagagtgg aattccagca
      481 agcgagagcc caaaatacac cagatacatc tgtatcaaca gcggcgtgtt ccggacgacc
      541 tggatgtata tcccggacag ccagcccaga ggcccgcgtg ttgcctggcc cagggctacc
      601 acaaggcccc agacaataca gatcgccatc gtcagaaccg cgagttcgac cgtcacctcg
      661 gcaccctcca gcaaggctgg aaacaggcgc gcgaggaaat tcaggtcgat atgcacgagt
      721 tagtatttgt tgaagtcata gccccgctgc ggcggcgcgt ccttgtgcag ccatgtctgg
      781 taccattcct ggtacttgtt gtagcgggag ccatagcgga gttcgcccac ccaggtgtcc
      841 aggaacagcc accacttgaa gtcgccgggt ttcatgaaga tcgcgtcctg tgccacgtag
      901 gcgaacggcg tgccggctac gtccaggcgc atcaggtctt cattctcatg ctcataaaaa
      961 tcggcgatcg gcttatcgat attgaatgca gtcgcacgcc cggtcttcac cgccaggaac
     1021 agcgccgagg ggctgtccag cagcagcgtc ttcaccttcg gcaaaagctg cttcgcccgc
     1081 tcgatcgccg gcggcacgtt caggatggca taggtgtatt tcggatcgtt gagttcctgg
     1141 atcgtcttga tcttggcatc cttccgagcg accaggctgt taccggtgtc gaggtaaggc
     1201 ctggtgaagg cgatccgtac agcccggtcc gggtagatgg tggtggagca caggccaaaa
     1261 tccaccttgc ccgacagggc ggccgggaac cggccgtccg actgcacgac cacgaactcg
     1321 accttttttg ggtcgcccag caggtccttg gcaatttcat gcgccatgtc gatctcgaat
     1381 ccgaccagat cgcccttgtc atcgacataa gccaacggcg gggatgtact gtaggtcgcg
     1441 acgatgatct tgtcgcgttt caacacggta tccagcagcg agtcctgtgc cctcgccgga
     1501 gcgatggccg acaaaatcag caggacggcg ggcagcaatt gcaatcggcg catgaggact
     1561 cccttttctc gacctgtagc gaaaaccgtg ccggccggac ttgtccagcc tcgggctcaa
     1621 ggatggttaa ttatttgctc aagggccgac agatcaggcg gttgaaggtg gacgaagcag
     1681 cggaccggca ggccgtcgga ttggcgatcg aagttcaccg gcaacttggg ttcaatctcc
     1741 tgctgccgat ttgttacgct tctccgccat ggattggcat tccgaggccg tccacgttta
     1801 gcctgaagga ggtatctttg ctacgagcac atgctgctga cggttgattg cgagcgatgt
     1861 cgtggaaccg ttcctggcga tttgagccct gacaccgggg cttgcctcac ccgaccagat
     1921 tctgtcctca tgcggccggc agcaaccagc aggacgacaa cacaatgtgg caattctgga
     1981 tcgatcgcgg cggcaccttc accgatatcg tcgcgcgcga tcccgagggc aaactttcca
     2041 cccacaagct gctgtcggag aacccggaac gctacaagga cgccgccatc gccgggatca
     2101 ggtcgatcct cggacttatg ccggatgccg ccatcccgcc caacctgatc gagtcggtca
     2161 agatgggcac gacagtcgcc actaacgcgc tgctggaacg caagggcgat cgcaccctgc
     2221 ttctggtgaa caaaggcttc gcggatgcac tgcgcatcgg caaccaggcc cgcccgcgcc
     2281 tgttcgacct ggcgatcacc ctgccgacga tgctgtacga acgagtggcc gaaatcggcg
     2341 gccgcgtcgg catggacggc caggagatcg aagcgctcga cgctgacctg gcccgcgaag
     2401 tttgtgcgaa agcccgagcg gacgggatcg atgcctgcgc catcgtgctg atgcacgcct
     2461 ggaagtatcc cgaacacgag caacgtctga cggacctggc acgcgaggcc ggcttcaccc
     2521 agatttccgc cagccatgcc gtcagcccgc tgttgcgcct ggtgcctcgt ggcgacacca
     2581 ccgtggttga cgcctacctg tcgcccatcc tgcgccgcta cgtcgatcag gtcgcatcgg
     2641 aactgagcgg cacccgcctg tatttcatgc agtccaacgg cggcctgacc gaggcgcaca
     2701 gcttccaggg caaggatgcc atcctgtcgg gaccggccgg gggcattgtc ggcgccgcgc
     2761 gcactgccgg catggccggg ctggaccaga tcatcggctt cgacatgggc ggcacctcga
     2821 cggatgtcgc actctatgcc ggatcctttg aacgcgcgtt cgaaacggcc gtcgccggcg
     2881 tgcgaatgcg cgcaccgatg atggcgatca acaccgtcgc ggccggtggc gggtcaatcc
     2941 tgcattttga cggcgcccgc atgcgcgtcg gccccgattc ggcgggcgcc aacccgggac
     3001 cggcctgcta tcgccgcggc ggcccactga cggtgacgga tgccaatgtc tgcgtcggca
     3061 agatccaacc cgcgcatttc ccgccgatct tcggcccgca tggcgaccag ccactcgacg
     3121 ccgaggtggt gacggggaaa ttcgccgcgc ttgccgggga aatcggcggc gaccggaccc
     3181 cgcggcaagt ggcggaaggc ttcctgcgga tcgccgtcgc caacatggcc aatgcgatca
     3241 agcaggtctc ggtgcagaag ggtcatgacg tgtcgcggtt cgcgctgcaa tgcttcggcg
     3301 gggccggcgg ccagcatgcc tgcctggttg ccgatgcgct gggcatggag acggtcttca
     3361 tccatcccta tgccggcgtg ctgtccgcct acggcatggg cctcgcggac cagaccacga
     3421 tgaaggagca ggcggtcgaa atccccctgg gcgccggcac gctggcggag atcgagacac
     3481 ttgccgagaa gcttgcaact gatgcgatcg gtgccctgga agcccaggga gccaatcccg
     3541 cagatgtcac caccgccaaa tccctgcatc tgcgctacgc cggtaccgaa gccgcactga
     3601 tcgttccttt gcgcgacctg gaaacgatta tatccgactt caccgccgcc caccgaaccc
     3661 gcttcggatt cgcgaccccg gaccggcccg tcgttgtcga aacggttgcc gcggaagcca
     3721 cattgcccgg ctccgtggcc acggaggcga ccttgcctgc ccgcgaaacc ggcgagcccg
     3781 ccatcatcga ccgggtccgc gtcttcaccg gcggcaccga gatcgaggca ccggtgctgg
     3841 agcggacctc gctactggcg ggcgatcgca tcgccggccc ggcgctggtc cgcgaggcca
     3901 acgccacaac cgtcatcgaa cccggttgga ccgccgagat caccgcgctc gaccacatga
     3961 tcctgcgccg gaccgaaccc ctgcccagcc gcgtgccggc cggcagcgaa cgccccgatc
     4021 cggtattgct ggaactgttc aacaacctgt tcatgaacgt cgcggaacag accggcgcgg
     4081 tactgcagaa cacatccatg agcgtgaaca tcaaggagcg actggatttc tcctgcgcca
     4141 tcttcgacgc cgacggcaac ctggtcgcca atgcgccgca cgtcccggtc catctaggcg
     4201 cgatgggtga aagcgtccgc accgtgctgg agcaccgtcg cggcaccctg aagccggacg
     4261 atgtcgtcgc gctgaacaac ccgttcaacg gcggcactca tttgccggac atcacggtca
     4321 tcacgccggt gttcgacgcc ggcggaacgg agattttgtt ctttgtcggc agccgcggcc
     4381 atcatgccga catcggcggc atcacccctg gctccacccc gccaatgtcg cgcacgctgg
     4441 aagaagaagg cgtcgtcatc gacgatttcc tgttggtgga cggcggacat ttccgcgaaa
     4501 ccgagtttcg ggcgttgctc gccggggccc gctatccggc tcgcagtccg gatgtgaacg
     4561 tcgccgacgt caaggcgcag gtggctgcga acgaaaaggg cgtgcaggaa ctgcaacggg
     4621 ttgtcgccca gtacggctgg ccggtcgtca gtgcgtatat gcgccacgtt atggacaatg
     4681 ccgaggaatc ggtccggcgc gtaatcgaac gcctgggttc cggccgtttc gactacaaga
     4741 tggacaatgg ccgcgaccta tgtgtctcga tcagcgtcga tcgggaaaac cgtagtgcaa
     4801 cggtggattt taccggcacc gggccgcagc acgagggcaa cttcaattcg cccccggcgg
     4861 tgacccgagc ggcggtgctg tatgtgttcc ggtgtttggt gggggatgat attccgctga
     4921 acgacggctg cctcaagccg ctgaccttga tcattccacc gggaacgttc ctgtcgccgc
     4981 tccctggcgc ggctgtcgtc gcaggcaata ccgaggtttc acaggcaacc tgcaatgcca
     5041 tcttcggtgc gttgggcgtc atcgcctgct cccaggccac gatgaacaat ttcctgttcg
     5101 gcgacgcgac ccgccaatac tatgaaacca tctgcggcgg caccggcgcc gggccagggt
     5161 ttaacgggac gtcggccatc cagacccata tgaccaacac ccgcatgacc gatcctgaga
     5221 ttctcgaact ccgctatcct gttcgtcagg agttcttcgg catccggcac ggctccggcg
     5281 ggaaaggtaa gtggactggt ggggacggtt cgatccgccg catccgcttc ctggaaccga
     5341 tgaccgcggt gattgtctcc tcgcgtcgca aggtcgcgcc gtttggcctg gacggcggtt
     5401 cagatggtgc cgctgggcgg cagtgggtgg agcgggcgga tggatcgcga gaaacgctca
     5461 ccggcaccga cagcgcggaa ctggcgccag gcgatctgtt cgttatcgaa acaccaggtg
     5521 gcgggggcta tggacgagcc tagagtctgt tagctcgtaa tgtctttccc aatattgggg
     5581 ccatgacaaa tgaaatcggc tacggcgttt tgacagatgc ccaatgggct gagctggcac
     5641 cgctgatcga gcagtgccgc ccgccccaca agaccgcgca cgacaacctc cgacgcacca
     5701 tcgaggcgat catctggcgg cacgacaacg gcgcgaaatg gcgcagcatt ccgtcccatc
     5761 tggggccgtg gtgg
//
LOCUS       DASWQN010000039         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_795706, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000039 DASWQN010000000
VERSION     DASWQN010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795706"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VGC82_00635"
     CDS             <1..502
                     /locus_tag="VGC82_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0421812.1"
                     /translation="HWRGRKRHRQLSDIDRRRLDILPQQARLADLTVVRHPATPEEVS
                     SSDTLHAVLFDSGRPLLIAPQVAPTTIGRRICLGWNGSAESTAAVEAAMPWLQRAEAV
                     RILWAEEYQRRGPRAPELQAYLAMHGVPADLKMFKPIDNIVGAGLLRACQGSIARSER
                     LEGLMV"
     gene            complement(454..1680)
                     /locus_tag="VGC82_00640"
     CDS             complement(454..1680)
                     /locus_tag="VGC82_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEY0421813.1"
                     /translation="MIDPVPIRDRFTALSPHLNERDRRLFAATEAATAGYGGIAAVSA
                     ATGIAVSTIGRGLKDLVEAGGLLAGRVRRSGGGRKSLVARNPGLLPDLMALVEPGERG
                     DPMSPLRWTCKSLRQLATELVARGHQVSRTVVGELLKAQKFSLQANRKTNEGGDRPDR
                     NAQFSHINQRVTAALAAQQPVISVDTKKKELVGDFKNAGREWRPQGQPEEVRVHDFLI
                     KELGRAVPYGIYDLAANAGWVSVGMNHDTSAFAVQSIRRWWQEIGRRRYPDAERLTIT
                     CDGGGSNGSRVRLWKLELQKLANELCIDITVHHLPPGTSKWNKIEHRLFSFITMNWRA
                     KPLVSYQVIIDLIGATTTKTGLKVICELDNTHYPKGIVVSDEQLAAINMVRADFHGEW
                     NYTIKPSNRSDRAIDP"
BASE COUNT          346 a          574 c          551 g          391 t
ORIGIN      
        1 ccattggcga ggccgaaagc gccaccgcca gctttctgat attgaccggc ggcggctgga
       61 tattctcccg cagcaggcgc ggctcgccga tcttaccgtg gtgcgtcatc ccgccactcc
      121 ggaagaggtg tcctcctccg atactctgca cgctgtgctg tttgattccg gccgccccct
      181 gttgattgcc ccccaggtcg cgccgacgac gattggccgg cggatatgcc ttggttggaa
      241 cggctctgct gagtccaccg ccgctgttga ggcggctatg ccttggctgc agcgcgcaga
      301 agctgtgcgc attctctggg ctgaagaata ccagcgccga ggtccgcggg cgccagaact
      361 tcaagcctat ttggcgatgc atggcgtgcc tgctgatttg aagatgttca aaccaatcga
      421 caacatagta ggagccggcc tgctaagggc ctgtcaggga tcaatcgctc gatctgagcg
      481 attggaaggc ttgatggtat agttccattc gccatgaaaa tctgcccgga ccatgtttat
      541 ggcggccagt tgttcgtcgg agacgacgat gcctttggga taatgggtat tgtcgagttc
      601 gcagatcacc ttcaggccgg tcttggtcgt ggtggccccg atcaggtcga tgatgacttg
      661 gtagctgacc agcggcttgg cacgccagtt catggtgatg aaggaaaaca accgatgctc
      721 aatcttgttc cacttgctgg ttcccggcgg caggtggtgg accgtgatgt caatgcaaag
      781 ctcgttggcc agcttttgta gttcgagttt ccacagccgc acgcgagatc cgttgctgcc
      841 accgccgtcg caggtgatcg tcagacgttc ggcgtcgggg tagcgccggc ggccgatctc
      901 ctgccaccag cggcggatcg actgtacggc gaacgcggag gtgtcatggt tcatgccgac
      961 gctgacccaa ccggcgttgg cagccagatc atagatcccg tacggcaccg cccgccccag
     1021 ttccttgatg agaaaatcat gcacgcgaac ctcttcgggc tggccttgcg gacgccattc
     1081 ccggccagcg tttttgaaat ctccaaccag ctcctttttc ttcgtatcga ctgaaatcac
     1141 cggctgctgt gcggccaacg cggccgtcac gcgctgatta atatggctga attgcgcatt
     1201 ccgatcggga cgatcgccac cctcattggt cttgcggttg gcttgcagac tgaacttctg
     1261 tgctttcagc aattcaccga ccacggtgcg actgacctga tgcccacggg ccacaagctc
     1321 cgttgccaac tgtcgcaagc tcttgcaggt ccagcgcaac ggcgacatcg gatcgccccg
     1381 ttcgccaggt tcgaccagtg ccatcagatc aggcaacaag ccagggttcc tcgcgaccag
     1441 cgacttgcgt ccgccacctg aacgccgtac ccggcctgcc aatagaccgc ctgcctcaac
     1501 caggtctttc aggccgcgtc cgatcgtact gaccgcaatg ccggtcgctg ctgagacggc
     1561 ggcgatccca ccataccctg ccgtggcggc ttccgtggca gcaaaaagac gtcgatcgcg
     1621 ctcgttgagg tgaggcgata gagcggtaaa gcggtcgcga atcgggactg gatcgatcat
     1681 ggcgcaacat taccgaccgc cgcttctgtc gagaatcccg cctctatagg tcagccggcc
     1741 gcgagtcgat tgttcccgga caggccctta gggtaccgga gcggcaggcc gcatgacacg
     1801 cttccatcgt ggccgttctg atccggggca tgccccggat cagaacaaga acgaagcgga
     1861 ca
//
LOCUS       DASWQN010000040         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_927661, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000040 DASWQN010000000
VERSION     DASWQN010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927661"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VGC82_00645"
     CDS             complement(<1..1260)
                     /locus_tag="VGC82_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEY0421814.1"
                     /translation="MRDAFAIGPVLTLRPLRSHQQRLVGVVAAIASGQTEARDILAAV
                     TPGGGKSLLPVLAAAQLIAAGVVERICWVVPRDSLRLQAEEAFADPAWRSALGHSLSV
                     RAADNTPDPSRGVAGYITTYQGVAAAPDLHLSEFRRYRTLLVVDEVHHLPALAETDPT
                     TAAQTEEGGEDPASGWSRALLPLLECAAVRLLLSGTLERTDGKGILWLPYRTGPKART
                     REVELDAPGWAVIGYSRRQALAERAVLPVTFGALDGEATWRDENGIEAGPHRLGGSYP
                     VETTRPALFTALRTGFAEALLREAFLAIRDLRARRRQERGLAPGTAARGLGKLLVVAP
                     DQTAARHYLEIVRGWIPAAQADQVAQLATSDMAGAHEILAAFRLWPEPSVLVTVAMAY
                     EGLDVPEVAVVAALTHIRSRPWLEQMVA"
BASE COUNT          275 a          565 c          512 g          231 t
ORIGIN      
        1 cgctaccatc tgttcaagcc agggccgcga gcggatatgc gtcagtgccg ccaccacagc
       61 cacttccggt acgtccagcc cttcatacgc catggcgacc gtaaccagaa ccgacggttc
      121 cggccatagc cggaaggcgg ccaggatctc gtgcgcaccc gccatgtcgg acgtcgcaag
      181 ttgcgccacc tggtccgcct gggcagccgg gatccaacct cggacgatct ccagataatg
      241 ccgagccgcg gtttggtccg gcgccaccac cagtagtttg ccaagtccgc gcgcggcggt
      301 tccgggtgcc agtccacgct cctgccgccg gcgcgcgcgc aggtcgcgga tggcgaggaa
      361 cgcttcgcgc agcagcgcct cggcaaagcc ggtacgaagc gcggtgaaca acgccgggcg
      421 cgttgtttcg accgggtagc tgccgcccag ccgatgcggg cccgcctcga tgccattttc
      481 atcgcgccac gtcgcctctc catccagcgc gccgaaggtc acaggcaaga cggcacgttc
      541 ggccagcgcc tggcggcgtg agtagccgat gaccgcccat cccggcgcat cgagctccac
      601 ctcccgcgtg cgcgccttcg gtcccgtgcg atacggcagc cacaggatgc ccttcccgtc
      661 cgtccgctcc agcgtgccgg acaacaacag gcggacggcg gcacactcca gcaacggcag
      721 cagggcgcgc gaccagccgc tggccggatc ctctccgcct tcctccgtct gcgccgctgt
      781 cgtggggtcg gtttcggcca gcgccggcag gtggtgtacc tcgtccacca ccagcaaggt
      841 ccggtagcgt ctgaactcac tcagatgcag atcgggagcc gcggcaacgc cctggtaggt
      901 ggtgatgtag ccggcaacgc cgcgactcgg atcgggcgtg ttgtcggccg cccgaacgga
      961 gaggctgtgt cccagtgccg aacgccaggc cgggtcggcg aacgcctcct cggcttgcag
     1021 ccgcaggctg tcgcgcggca ccacccaaca gatccgctcg accacgccgg ccgcgatcaa
     1081 ctgggcggct gccagcaccg gcagcagcga ctttcccccg ccgggggtca ccgctgcgag
     1141 gatatcgcgc gcctcggtct gtccactggc gatggcagcg acgacgccga cgaggcgctg
     1201 ttgatgggaa cggagcgggc gaagcgtcag gacaggacct atagcgaaag cgtcacgcag
     1261 agtcttgagt cctcaggcct cggctgtcca gccacatcat atcggcaagc cgaaagtttt
     1321 tcgtagattt atggcggcaa cacagtccaa cggcacgtag gccgtgccag aaacaccggt
     1381 ccaacgcaca tggcttccca cttaccccgg gaaagtccta atcatgggaa gaacgggagg
     1441 aacacggcga tgccagcacg caccggggcg gaattcctgc acggcctgcg cgacgaccgc
     1501 gaactctggc tgggcggtca gcgcgtcagc gacccgatgg agcacccggc actccgtggc
     1561 gcggcccggg ccatcgccga cgt
//
LOCUS       DASWQN010000041         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_531858, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000041 DASWQN010000000
VERSION     DASWQN010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531858"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VGC82_00650"
     CDS             complement(<1..656)
                     /locus_tag="VGC82_00650"
                     /inference="COORDINATES: protein motif:HMM:NF037542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0421815.1"
                     /translation="MPKNPAGASMAGHIRPAMASVRRDLGCLFAAGFQFRLIVIGCPL
                     NPCNNSPALDRSLDMSSNPFFSDLLASISERGRTLLRRAGPSDGKHDAAGLVELCEAL
                     LSGRGEASGTAMAREVLDRYRHLDEASRLSFFETLARGYGPDRERLARAIENWRTQPN
                     DADASDLHFASEPRRQELIRRLNRAPGGTSELVAMRADLLRLMNGHKDLAALDRDVVH
                     "
     gene            717..1892
                     /locus_tag="VGC82_00655"
     CDS             717..1892
                     /locus_tag="VGC82_00655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0421816.1"
                     /translation="MNSPSRVIAFVNAAHFIDHYAMLIFAAAVIVMGPALGMAYSELL
                     PYATPGFIAFGAGSLLTGWLGDRWSRRHMMVIFFAGIGASMIAVGVVQTPLQLGVALL
                     SIGLFASIYHPVGTAMIVSYADRLGREMGLNGVFGNLGVASSALVTGVVGQFLGWRWA
                     FILPGIVTILIGLVFARSVSHEDRSGHKQAAAQARIAKNDMWRVLLALLIVVIAISTT
                     FNAVTVALPKLFAERLSDLTRSPALLGAIAACVYVFGAMTQYTIGKLIDRYSLKAVSL
                     PLSFLLAPFLYLAATLSNLPLIIAAIGIVMGAFGQVTVNDAMVGKYTSEQWRSRAYSV
                     RYFVGFTAAGASVGMVAWLYEQGGFVTMLHAFAGLCLLVIAAAIILPPEIKVPAAGR"
BASE COUNT          345 a          663 c          717 g          401 t
ORIGIN      
        1 aaatgcacga catcgcgatc aagcgccgcg aggtccttgt ggccgttcat cagacgaagc
       61 aggtcggcgc gcatcgccac cagttcgctg gtgccgccgg gcgcgcggtt gagccggcga
      121 atcagttcct gccgccgcgg ctcggaagca aaatgcaggt cactggcgtc tgcatcgttg
      181 ggctgcgttc gccagttctc gatcgcccgt gccagccgtt cgcggtccgg accgtagccg
      241 cgcgccagcg tttcgaagaa agagaggcgg ctggcctcgt cgaggtgacg gtagcggtcg
      301 agaacctctc gcgccatcgc ggtgccggag gcctcgcccc ggcccgacaa cagcgcctcg
      361 cacagttcga ccaggccggc cgcgtcatgc ttgccgtcgg acggcccggc gcggcgaaga
      421 agggtccggc cccgctctga aatcgaggcg agaaggtcgg aaaagaaggg gttactgctc
      481 atgtcgaggc tgcgatccag ggcgggggaa ttgttgcatg gatttaaggg acagccgatc
      541 acaatcaagc ggaattgaaa ccccgcggca aataaacagc ccaggtcgcg gcgcacggac
      601 gccatcgccg gccggatatg gccggccatg ctggcaccgg ccgggttttt cggcaatatt
      661 ggccggccgg tcccttcaag aattcgtacc gaacgccggc cccaggatct agatccatga
      721 acagtccgtc ccgggtgatt gctttcgtca acgcggcgca tttcatcgac cattacgcga
      781 tgctgatttt cgctgccgcg gtgatcgtga tggggccggc gctcggcatg gcttattccg
      841 agctgctgcc ctacgccacg ccgggcttca tcgcgttcgg cgccggatcg ctgctaaccg
      901 gctggctcgg cgatcgctgg agccgccgcc acatgatggt gatctttttc gcgggcatcg
      961 gagcttcgat gatcgcggtt ggcgtggttc agacgccgct gcagctcggc gtggcgctgc
     1021 tctcgatcgg cctgttcgcg tcgatctatc atccggtcgg caccgccatg atcgtatcct
     1081 acgccgaccg gctgggccgc gagatgggac taaacggtgt gttcggcaat ctcggcgtgg
     1141 cgtcgtcggc gctggtcacc ggcgtggtcg gccagttcct gggctggcgt tgggctttca
     1201 tccttccagg catcgtcacc atcctgatcg gtttggtctt cgcgcgctcg gtgagccatg
     1261 aggaccgctc cggccacaaa caggcggcgg cgcaggcgcg catcgccaag aacgacatgt
     1321 ggcgggtgtt gctggcgctg ctgatcgtgg tgattgcgat atcgaccacc ttcaacgcgg
     1381 tgacggtcgc gctgcccaag ttgttcgccg agcggctgag tgacctgacc agaagcccgg
     1441 cactgctggg ggcgatcgcg gcgtgcgtct atgtgttcgg agcgatgacg caatacacca
     1501 tcggcaagct gatcgaccgc tattcgctga aggcggtttc gctgccgctg tcgttcctgc
     1561 tggcgccatt cctgtatctg gcggccacct tgtcgaacct gccgctgatc atagccgcga
     1621 tcggcattgt gatgggagca ttcggccagg ttaccgtcaa cgacgccatg gtcggcaagt
     1681 acaccagcga gcaatggcgc tcgcgggcct attcagtgcg gtatttcgtc ggcttcaccg
     1741 cggccggcgc ctccgtcggg atggtggcgt ggctgtacga gcagggcggt tttgtcacga
     1801 tgctacacgc gttcgcaggg ttgtgcctgc tggtgatcgc cgcggcaatc atcctgcctc
     1861 cggaaatcaa ggtaccggcc gcgggccgtt agttatcgcg aagcgccggt agccagcccc
     1921 ggggttgtac agatgacggt aggggcgcgt cagcgcgggg cgccctgggt cgcggccgcg
     1981 aacgacgtca gcggccattc gctggacatc agcctgccgg cgaccacgat cagggtcacg
     2041 gcaagcaaca gccgcagcgc cgtttccggc acgcgggtgg cgaaatagct gccgatcacg
     2101 atgccgggca gggaacccat cagcag
//
LOCUS       DASWQN010000042         3343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_4120, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000042 DASWQN010000000
VERSION     DASWQN010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4120"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /gene="pufM"
                     /locus_tag="VGC82_00660"
     CDS             complement(<1..951)
                     /gene="pufM"
                     /locus_tag="VGC82_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009521 - photosystem [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center subunit M"
                     /protein_id="HEY0421817.1"
                     /translation="MAEYQNIFTRVQVRGPVHGGIALPRGSWNRLGKPFQSRLLGLIG
                     DAQVGPIYLGFAGVASLICGFFAIEIIGLNMWASVNWDPIQFIRQLPWLALEPPPPAY
                     GLTILAPLAQGGWWQMAGFFLTTSILLWWVRMYRRAIALGLGTHVPWAFASAIWLYLV
                     LGFIRPIMMGSWAEAVPFGIFPHLDWTAAFSIRYGNLFYNPFHMLSIVFLYGSAVLFA
                     MHGATILAVSRFGGEREVGQITDRGTAAERAALFWRWTMGFNATFESIHRWAWWFAVL
                     CPLTGGIGILLTGTVVDNWYLWGVKHGIAPMYPQIFEHVTD"
     gene            complement(973..1800)
                     /gene="pufL"
                     /locus_tag="VGC82_00665"
     CDS             complement(973..1800)
                     /gene="pufL"
                     /locus_tag="VGC82_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009521 - photosystem [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center subunit L"
                     /protein_id="HEY0421818.1"
                     /translation="MAMLSFERKYRVRGGTLIGGDLFDFWVGPFFVGFFGVTTAFFSI
                     LGTALIVWGAAIGPTWNVWRINIPPPDLSYGLAFAPLQEGGLWQLITICATGAFCSWA
                     LRQAEIARKLGMGMHIPFAYAFAILAYVCLVIVRPLLMGAWGYGFPYGIMSHLDWVSN
                     TGYQYLHFHYNPAHMLAVSFFFTNALALALHGGLILSAASPGPGLTVKSAEQENAYFR
                     DTIGYSIGTLGIHRLGLFLAVSASFWSAVCMIISGPFWTRGWPEWWSWWLNLPIWAH"
     gene            complement(1910..2095)
                     /gene="pufA"
                     /locus_tag="VGC82_00670"
     CDS             complement(1910..2095)
                     /gene="pufA"
                     /locus_tag="VGC82_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="light-harvesting antenna LH1, alpha subunit"
                     /protein_id="HEY0421819.1"
                     /translation="MWRLWLLFDPRRVLVALSVFLFVLALLIHFILLSTDRFNWLEGP
                     HHGVNAAQMAPLPPAVK"
     gene            complement(2108..2332)
                     /gene="pufB"
                     /locus_tag="VGC82_00675"
     CDS             complement(2108..2332)
                     /gene="pufB"
                     /locus_tag="VGC82_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030096 - plasma membrane-derived
                     thylakoid photosystem II [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="light-harvesting antenna LH1, beta subunit"
                     /protein_id="HEY0421820.1"
                     /translation="MTPTSTSVTGLTEPEAKEFHGIFITSFIVFTVIAIVAHFLAWQW
                     RPWLPGPRGYATSWLYDAGGTIVHTISQLV"
     gene            complement(2468..>3343)
                     /locus_tag="VGC82_00680"
     CDS             complement(2468..>3343)
                     /locus_tag="VGC82_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HEY0421821.1"
                     /translation="TFPLGAHIDDIQRLPQADVNVCMYREFGRKLCEELDRPYLQAPI
                     GLFSTTNFLRTLGKLTGADPEPFIEREKHTTIKPIWDLWRSVTQDFFATASFGAVATD
                     TYARGLQRFLQDEMGIPCTLAASRCAGTKPDNAAIRAALRAKPPLVLFGSYNERMYAA
                     EAGNRSMYIPASFPGAIIRRATGTPFMGYSGATYIVQEFCNALFDALFNILPLGTDLD
                     RIEPTPSRLSASCPWDEDAQTLFDDYLETEPFLVRISAAKRLRDRVEAAVRGAGEDRV
                     TAGHVSDALAARQPA"
BASE COUNT          712 a         1017 c         1049 g          565 t
ORIGIN      
        1 atcggtcaca tgctcgaata tttgcgggta catcggggct atgccgtgtt tgacgcccca
       61 caggtaccag ttatcgacga ccgttccggt cagcaggatg ccgatgccgc cggtcagcgg
      121 acacagcacg gcgaaccacc aggcccagcg atggatcgat tcgaaggtgg cgttgaagcc
      181 catcgtccag cgccagaaca gcgcagcgcg ttccgccgcg gttcctcgat cggtgatttg
      241 tcccacctcg cgctcgccac cgaagcggct gaccgcgagg atggtcgcgc catgcatcgc
      301 gaacaacacc gccgatccat aaaggaacac gatcgaaagc atatgaaacg ggttatagaa
      361 cagattcccg tagcggatcg aaaatgccgc ggtccagtcg aggtgcggga agatgccgaa
      421 cggcacggcc tcggcccagc ttcccatcat gatcgggcga atgaagccca acaccaggta
      481 cagccagatc gcggacgcga atgcccaggg gacatgcgtg ccaaggccga gggcgatcgc
      541 tcgccgatac atccgaaccc accatagcag gatggatgtg gtcagaaaga acccggccat
      601 ctgccaccag ccgccctgcg cgagcggagc caggatcgtc aggccgtagg ccggtggtgg
      661 cggttccaga gccagccagg gcaattgccg gatgaactgg atcgggtccc agttgaccga
      721 ggcccacatg ttcaggccga tgatctcgat ggcgaagaag ccacagatca acgacgcgac
      781 gccggcaaac cccagataga tcggtccgac ctgggcatcg ccgatcaggc ccagcagacg
      841 ggactgaaac ggttttccga gccggttcca gctgccgcgc ggcaatgcga tgcccccgtg
      901 cacgggtccg cgaacctgca ctcgggtaaa gatgttttga tactctgcca ttgtttcctc
      961 cccgcttccg gatcaatgcg cccagatcgg aagattgagc caccagctcc accattcagg
     1021 ccacccacgc gtccagaacg ggccactgat aatcatgcac acggcgctcc agaacgatgc
     1081 cgacacggcc agaaacagcc ccaggcggtg aatacccagc gtgccgatcg agtaaccgat
     1141 cgtatcgcgg aagtaggcat tctcctgttc cgccgacttc actgtcaggc cggggccggg
     1201 actggcggcc gatagtatga gtccgccgtg cagcgccagc gccagcgcat tggtaaagaa
     1261 gaaactgacc gcaagcatgt gcgccgggtt gtagtggaag tgcagatact ggtatccggt
     1321 gttcgatacc cagtcgagat ggctcatgat gccatagggg aaaccgtagc cccaggcgcc
     1381 catcagcagc ggccggacga tcaccaggca cacgtaggcg aggatggcga aagcgtaagc
     1441 gaacgggatg tgcattccca tgccaagctt gcgggcgatc tcggcctggc gcagtgccca
     1501 ggagcagaac gccccagtcg cacagatggt gatcaactgc caaagcccgc cttcctgcag
     1561 tggcgcgaaa gccaggccat aactaaggtc cggcggtggt atgttgatgc gccaaacatt
     1621 ccaggtcggg ccgatcgccg cgccccaaac gatcagggcg gtgccgagga tcgagaagaa
     1681 cgccgtcgtt acgccaaaaa atccgacaaa gaacggtcct acccaaaaat cgaacaggtc
     1741 accgccgatc agggtgccac ccctgacgcg gtatttcctt tcaaaactga gcatagccat
     1801 cgtcagctct cctatcgccg gatcgtcgcc aggcgtatgg atggaaggcg ggaagcggtg
     1861 acgggcaagg cgcgcaggat gccacgcccg ccaccgcggc cggacacggt tatttcacag
     1921 cgggtggcag tggtgccatc tgcgccgcgt ttacaccgtg atgcggacct tccagccagt
     1981 tgaaccggtc ggtgctgagt agaatgaagt gaatcagcaa cgccaggacg aacaggaaga
     2041 cgctcaaagc gacgagcacc cgcctggggt cgaacagcag ccaaagtctc cacatgttcg
     2101 cttcctccta aacgagttgc gagatcgtgt gaacgatcgt tccgccggcg tcatacagcc
     2161 atgatgtcgc atagccgcgt gggccgggca gccaaggacg ccactgccac gcgaggaaat
     2221 gcgcgacgat ggcgattacg gtgaagacga tgaagctcgt gataaagata ccgtggaact
     2281 ctttcgcctc cggctctgtc aaccccgtta ccgaggttga cgttggggtc atagccatgt
     2341 ggttactctc cagtctcggc ctcgcggccg ttgtcgcgcc catcccgcgc gactgggtgt
     2401 gccgcgaaga cgggccgcgg cgcccattcc gctgagtttg tcagcggaat ctcgatacaa
     2461 ccgacgatca tgcgggctgc ctcgcggcaa gcgcatcgga aacatggccg gcggtgacgc
     2521 gatcctcgcc cgccccccgt accgctgctt cgacacgatc gcgcagtcgt ttcgcggcgg
     2581 agatgcgcac caggaacggt tcggtctcca ggtaatcatc gaagagtgtt tgcgcgtctt
     2641 catcccacgg gcagcttgca ctcagccgtg acggcgtggg ctctatcctg tcgaggtcgg
     2701 tgccgagcgg caggatgttg aacaatgcgt cgaacagggc gttgcagaac tcctgcacga
     2761 tgtaggtcgc gcctgaatag cccatgaatg gggtgccggt ggcgcggcgg atgatggcgc
     2821 ccgggaagga cgccggtata tacatgctgc gattgccagc ctcggccgcg tacatccgtt
     2881 cgttgtacga tccgaacagc accagcggcg gcttggcgcg aagtgccgcc cgaatcgcgg
     2941 cattgtctgg cttggtgcca gcgcagcgcg atgcggcgag ggtgcacggg atgcccatct
     3001 catcttgcag gaagcgttgc aatccgcggg cataagtgtc cgtcgcgacc gcgccgaacg
     3061 acgcggtcgc gaagaaatcc tgcgtcacgc tgcgccacag atcccagatg ggcttgatgg
     3121 tggtgtgctt ctcgcgctcg atgaacggct ctggatcggc cccggtcagt ttgcccaggg
     3181 tccgcaagaa gttggtggtg gaaaaaagcc cgatcggcgc ctgcaaatac ggacggtcaa
     3241 gttcctcgca gagcttgcgg ccgaattcac ggtacatgca gacattcacg tcggcttggg
     3301 gaaggcgttg aatgtcgtcg atgtgggcgc cgagcgggaa ggt
//
LOCUS       DASWQN010000043         4716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_268133, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000043 DASWQN010000000
VERSION     DASWQN010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268133"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VGC82_00685"
     CDS             <1..239
                     /locus_tag="VGC82_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421822.1"
                     /translation="SYRATALGFLLILASKDVKDTALPGRACNLKSPAHTQGGYRGAK
                     NLTQSKNLTQSKISLGIVRLPILYLFVRPHNRAR"
     gene            complement(256..3075)
                     /locus_tag="VGC82_00690"
     CDS             complement(256..3075)
                     /locus_tag="VGC82_00690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003618204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY0421823.1"
                     /translation="MTASLALPDAPSVVAGHGRAAILTPDGELLILPAPQAAELLRTL
                     PPPILVHAPATFRRLGMRPGPAFDLLELFAFVRPAQTAAPTPRGLAMALDDDHPPIGL
                     EAEVTRLADLAAALLNQLFQGRDTLRNRDAAVLAARMQKAGWGWGGYVTHALGRPDAA
                     SSNEPLKVWKKLPDWEDSAPLPPPSAHPVSEAEARVRLAAMLGPHAEQRPGQGDYAGA
                     ATAAFAPREVRGDPHLVLVEAGTGTGKTLGYLAPASVWAEKNKGAVWISTFTRHLQRQ
                     IDGELERLIPDPTERRRRIVVRKGRENYLCLLNLEEAVSSASGGFMNQQVIPLGLIAR
                     WAMATDDGDVQGGDLPGWLSELFGGGLIASLADRRGECIHAACPHWRRCFIEHTIRRS
                     RTAELVVANHALVMTQAAWGGLDDNAVPSRYVFDEGHHVFDAADSAFSASLSGGETAE
                     LRRWLRGAEGGRSRARGLRKRLEDLVADRPALVAPLEAALQASSALPPSDWASRMQEE
                     GAELEGVEPARPNPSEAFLRLIRRQVLARTAGSDARPAFGSPAFGAVECDVFPLHEDA
                     AAAADRLARALSRITEPLATLRERLLARLDDEAEDMDEATRNRIEAIGRSLTRRALNP
                     LQAWQSMLWSLTEPQPDPGQRPTHVLFLRLDRRDGLRDVDVGLHRHWLDPTIPFAATL
                     ASPAQGLLITSATLRDSGDHDPEVAWASAEARVGAPHLPSPSIRAAVASPFDYAEQTR
                     AFVVSDVAPGNLDQLAAAYRALFLASEGGALGLFTAISRLRAVHAKIAPELEQAGFPL
                     YAQHVDAMGNATLVDIFRAEEESCLLGTDAMRDGVDVPGNALRLVVFERTPWPRPDIL
                     HRERRVHLSDGDPKGYDDRIVRLRLRQAFGRLIRRTSDRGVFVLLDRQAPTRLMSAFP
                     PGVVVQRVGLARAVAETGAFLRSRA"
     gene            complement(3310..4254)
                     /locus_tag="VGC82_00695"
     CDS             complement(3310..4254)
                     /locus_tag="VGC82_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421824.1"
                     /translation="MVIANREYGGHHSLTVRAMESAPLVSATRWNCRGGTELEVDPRG
                     WSWTWLSPDVIVDTDRDGIRFPGVLPGMVNRLRLRLRNRGNAVASDIRVKFWWQPAGP
                     QLSSWAWTPVAEASCVRLQPNDEEWVAVEWVPPPEQPGLSGWCIKAEIVVPNDPNTDD
                     KIVLSGVAGVWDAVPDEASEAGGASTWAGGDTRTELRLLARTPGYRCTITPAPANGDA
                     KARTRQFVVTDGIARPADPLPRDIKLAFVPSDDLTEWNGQRETCLPESKVFYPVVPGT
                     LPENLQRDGAMALATLVSVVDGLAVCGATFILPGRPGK"
     gene            complement(4333..>4716)
                     /locus_tag="VGC82_00700"
     CDS             complement(4333..>4716)
                     /locus_tag="VGC82_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421825.1"
                     /translation="PDRREVVSQETTWGNWLVANYLRGMKSATEDRRFQYQEDNEATP
                     PQRTQYWTSELLHARVLPGDAQTLDPAGEIRFNPSDREPPCWGGARYYRVLLTNRASL
                     VKISVATPDGSKADPVTQVLLLGPD"
BASE COUNT          783 a         1552 c         1629 g          752 t
ORIGIN      
        1 gatcgtatcg cgcgaccgca ttgggatttc tcctgatctt ggcttcaaaa gatgtaaagg
       61 atacagcgtt gccgggtcga gcgtgcaacc taaaatcgcc cgctcacact cagggcggtt
      121 atcgcggcgc taaaaatctc acccagtcta aaaatctcac ccagtctaaa atctcacttg
      181 gtatcgtcag gctgccaata ctctaccttt tcgttcgacc gcataatcgt gcgcggtaag
      241 cgttccaccg cgcgatcatg ccctggaccg caggaaagcg ccagtctcgg cgaccgcgcg
      301 cgccaagccg acccgctgca ccaccacacc cggcgggaag gcagacatca gccgcgtcgg
      361 ggcctggcga tccagcagca cgaatacacc gcgatcggag gtccggcgga tcagccgccc
      421 gaacgcctgg cgcagccgca gccgcacgat tcggtcgtca taacccttgg ggtctccgtc
      481 cgacaggtga acccgccgct cgcgatgcag gatgtcgggg cgcggccacg gcgtgcgctc
      541 gaacaccacc agtcgcaggg cgttgccggg aacgtccacc ccgtcgcgca tggcgtcggt
      601 gccaagcagg cagctttcct cctcggcgcg gaagatgtcc accaatgtgg cattgcccat
      661 cgcatcgacg tgctgcgcat acaacgggaa gccggcctgt tccagctccg gcgcgatctt
      721 cgcatggacc gcgcgcagcc ggctgatcgc ggtaaacagg ccaagcgcgc cgccttcgga
      781 ggctaggaac agcgcccgat aagccgccgc aagctggtcg aggttgcccg gcgccacatc
      841 gctgaccacg aaggctcggg tctgttcggc gtagtcgaac ggcgaggcga cggcggcgcg
      901 aatcgacggt gacggcaggt gcggtgcgcc cacgcgggcc tcggccgagg cccaggcgac
      961 ctccgggtca tggtcgccgg agtcgcgcaa ggtggcagac gttatcagca gcccttgtgc
     1021 gggtgacgcc agggtggcgg cgaacggaat ggtcgggtcc aaccaatgcc ggtgcagccc
     1081 aacatccacg tcacgcaatc cgtcacggcg atccagacgc aggaacagca catgggtggg
     1141 gcgctggccg gggtccggct gtggttcggt caggctccag agcatcgact gccaggcttg
     1201 cagcgggttc aaggcacgac gggtcaagct gcggccgatc gcctcgatcc ggttgcgggt
     1261 ggcttcgtcc atgtcctctg cttcgtcgtc gaggcgggca agcagccgtt cgcggagcgt
     1321 ggcgagcggt tcggtaatac gggatagcgc gcgcgccagg cgatcggctg ccgcggcggc
     1381 atcctcgtgc aacggaaaga catcgcattc aacggcgccg aaggctgggg aaccgaatgc
     1441 cgggcgggcg tcggagccag cggtacgtgc cagcacctga cggcggatga gacgcaggaa
     1501 cgcctcggac ggattgggcc gagcgggttc gacgccctcc agttccgcac cctcttcctg
     1561 catacggctg gcccagtccg agggcggcag ggcgctgctg gcctggagcg cggcctccag
     1621 cggggcgacc aaggctgggc ggtcggcaac gaggtcctcc aggcgcttgc gaaggccgcg
     1681 ggcacgtgac cggccgccct ccgcaccccg cagccagcgg cggagttcgg cggtttcgcc
     1741 ccccgagagc gaagccgaaa acgcgctgtc ggcggcatcg aagacgtggt ggccttcatc
     1801 gaatacgtat cgggacggaa ccgcgttgtc gtccagcccg ccccacgcag cctgggtcat
     1861 caccaaagca tggttcgcca cgaccaattc ggcggtgcgg gagcggcgga tcgtatgttc
     1921 gatgaagcag cggcgccaat gcgggcaggc ggcgtggatg cactcgccgc gacggtcggc
     1981 gagcgaggcg atcaggccgc cgccgaacag ttccgacagc cagccgggca ggtcgccgcc
     2041 ctggacgtcg ccatcgtcgg tcgccatcgc ccagcgagcg atcaggccca acgggatcac
     2101 ctgctggttc atgaacccgc cggaggcgct gctgaccgct tcctccaggt tcagcaggca
     2161 caggtaattc tcccggccct tgcggaccac gatccgccgc cggcgttccg tcgggtcggg
     2221 aataaggcgc tcgagttcgc cgtcgatctg gcgctgcaag tggcgggtga aggtgctgat
     2281 ccacaccgcg cctttgtttt tctccgccca gacgctggcc ggggcgagat agccgagggt
     2341 cttgccggtg ccggtgccgg cctcgaccag taccagatgc ggatcgccac ggacctccct
     2401 tggcgcgaag gctgcggtgg cggcgccggc atagtcgcct tgtccgggcc gctgttcggc
     2461 atggggaccc agcatggcgg cgaggcgaac gcgggcctcg gcttcggaga cggggtgtgc
     2521 cgagggcggc ggcaacgggg cgctgtcttc ccagtccggc agttttttcc agacctttag
     2581 cggctcgttc gacgaagctg cgtcggggcg tccgagcgcg tgggtgacgt agccacccca
     2641 gccccagccg gctttctgca tgcgggcggc gaggacggcg gcgtcgcggt tccgcagggt
     2701 gtcacggccc tggaaaagct ggttgagcag cgcggcggcg agatccgcaa gacgggtgac
     2761 ctcggcttcc aggccgatcg gcggatggtc gtcgtcgagc gccatggcga ggccgcgggg
     2821 ggttggcgcg gcggtctgcg cgggccggac gaaggcaaag agttccagca gatcgaaggc
     2881 ggggccgggg cgcatgccca ggcggcggaa agtggcgggg gcatgaacca ggattggcgg
     2941 cggcagggtg cggagcagtt cggcggcctg tggtgcgggc agtatcagga gttcgccatc
     3001 cggcgtcagg atggcggcac gaccatggcc cgccactacg gatggtgcgt caggcagggc
     3061 gagggatgcg gtcacccggc ttatgtagcc gggaacgggt gcggtgtcat cgcgacaacg
     3121 cgcgcttcca ggctgtcgcg cccgatgggg ccaatcctgc cgcagaggcg ggcggcgcag
     3181 cgtgcgcagg ctagccagaa cgattgaacg tcggtggaca gacatcgaca ggccgccggg
     3241 ccgcgggctg ccaacccggg gcatccgatc gccggtccct cggtgcgttt ttccgaccgt
     3301 gatcggacgc tatttccccg ggcggccggg caggatgaac gtggcgccgc aaaccgccag
     3361 cccgtccacg accgacacca gggtagccag agccatggcg ccgtctcgct gcaagttctc
     3421 cggaagtgtt cccggcacca ccggatagaa aaccttggac tccggcaagc aggtttcccg
     3481 ctgcccgttc cactccgtca ggtcgtcgct aggaacgaac gccagcttta tgtcccgcgg
     3541 aagcggatcg gcaggccgcg caattccatc ggtgacgacg aactgtcttg tccgtgcctt
     3601 ggcgtcgccg ttggcgggcg cgggggttat cgtgcagcga tagccggggg tccgagccaa
     3661 cagtcgcagc tcggttcggg tgtcgccgcc tgcccacgtg gatgcaccgc cggcttcgct
     3721 ggcttcgtcc gggacagcat cccagacgcc ggcaacaccg ctcagcacga tcttgtcgtc
     3781 ggtgttcggg tcgttcggca cgacgatttc ggccttgatg caccagccgg acaaacccgg
     3841 ctgttccggt ggcggcaccc attccacagc cacccattcc tcgtcgttcg gctgcaaccg
     3901 aacgcacgat gcctccgcca ccggcgtcca ggcccaggac gaaagctgtg gtcccgccgg
     3961 ttgccaccag aacttcaccc ggatgtcgct ggcgacggcg ttgccccggt tgcgcagccg
     4021 caaccgcaac ctgttgacca tgccgggcaa cacgccggga aagcggatgc catcgcggtc
     4081 cgtatccacg atcacgtcgg gcgacagcca cgtccatgac catccgcgcg gatcaacctc
     4141 aagctcggtg ccgccgcggc agttccaccg cgtcgcgctg accagcggag cactttccat
     4201 cgcccgaacc gtcaggctgt ggtggccgcc gtattcgcgg ttggcgatca ccaccacgat
     4261 ttcctgaagc ccggcggcgt tgatggttcg cgtccattgc ggccggtcgg aacgaatgat
     4321 gtcgcgaacg cgctagtcgg ggccgagcaa caggacctgc gtcacgggat cggccttgga
     4381 gccgtccggc gtagcgacgg aaattttcac gagggaggcc cggttggtca gcagcacgcg
     4441 gtagtagcgg gcgccgcccc agcaaggagg ttccctgtcc gatgggttga acctgatctc
     4501 cccggccggg tccagcgtct gtgcgtcgcc tggaagcacg cgggcatgaa gcagttcgga
     4561 tgtccaatac tgcgtgcgct gcggcggcgt ggcttcattg tcttcctggt attgaaaccg
     4621 ccggtcctcg gtggccgact tcatgccccg caagtaattt gccaccagcc agttgcccca
     4681 ggtcgtttcc tgcgaaacaa cctcgcgacg atcggg
//
LOCUS       DASWQN010000044         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1191738, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000044 DASWQN010000000
VERSION     DASWQN010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191738"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..431
                     /locus_tag="VGC82_00705"
                     /pseudo
     CDS             177..431
                     /locus_tag="VGC82_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116814.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(438..836)
                     /locus_tag="VGC82_00710"
     CDS             complement(438..836)
                     /locus_tag="VGC82_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421826.1"
                     /translation="MNVEFVATDWGSVVQRRTSKEPVEKGGWSVMHTWRPSTIGYTPM
                     EHSQIRGLGATAWFGWYKDEEMEVLTRRFVESTDPKEQDALVLAIQKRAFDQVPYVPI
                     GTFQIRKAYRKNLVGMVEGTAPYFWNIRRV"
     gene            complement(848..1999)
                     /locus_tag="VGC82_00715"
     CDS             complement(848..1999)
                     /locus_tag="VGC82_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0421827.1"
                     /translation="MLQGAVAAPFVPAKFAIAQPASARTLVFVPQANLTLLDPIFTTA
                     QPTVHHGWAIYDLLFAVTSKLEVKPQMAEGYTLSDDSRTYMIKLRNGLKFHNGEPVRA
                     QDCAPSLVRWARRETIGQTIWQYVDDCVAVDDRTIKITLKQPIAVFLEAIARGGASVA
                     FMMPEHVAKTDPFKQITEYIGSGPYRFLPDEYNSGAHVGYARFDDYVPRQEKAEWMSG
                     GKVAHFDRIEWRVIPDSATAAAALQSGEVDWYEQVQPDLVPLLKKNPAIEIGFHNPTG
                     YNGVLRFNHLHPPFNNVAVRRAVMMAVNQDDYMASVTAGDASAYTVCKSVFPCGSRYG
                     VEVGGEAMQANIAKAREMLAASGYKGEKAVLISPTDLTTVGLAMSPMTC"
     gene            complement(2096..2203)
                     /locus_tag="VGC82_00720"
                     /pseudo
     CDS             complement(2096..2203)
                     /locus_tag="VGC82_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345585.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          422 a          691 c          671 g          465 t
ORIGIN      
        1 gcagtagcct ctctgcgctt gtggcgttgg tggaggagag tgcgctgatg ttgtatttta
       61 accgccccga tgcatgagca agaactcacc tcggcagacc tcaccctcca ctgcaggccg
      121 gcgctcgacg cgtgagcctg atcgcgaaat gccggccgtc atcgcgtgtc gtcagcatgt
      181 ctgctcatct ggcaaattgc tgcccgtggg cgattcggcc cgggaggatc atccacatcg
      241 acataatgcc gtctatgctt tcgtagagca acgcgacaac tcgcgcctgc agggcattcc
      301 gtcggcccgt tctgtgttgg cgcgaacgcg gggtcgtagc ggcggcaagc tatgaagcca
      361 ggcgcttcgg cgtccgctcc gccacaccat cgattatggc acagctgcag cgccataatg
      421 tgatggtgcc gatggcgtca gacgcgccgg atgttccaga aatacggtgc cgtgccctcc
      481 accataccga caaggttctt acgataggcc ttgcggatct ggaatgtgcc gatcggcacg
      541 tagggaacct ggtcgaaggc ccgcttttgg attgccagca cgagagcgtc ctgttccttc
      601 gggtcggtgc tctcgacgaa gcggcgggtc agcacctcca tctcctcgtc cttgtaccag
      661 ccgaaccaag cggtagcccc gaggccgcgg atctgactgt gctccatggg cgtgtagccg
      721 atggtcgatg gccgccaggt atgcatcacg gaccagccac ccttctctac tggttcctta
      781 ctggtacgcc gctgcaccac gctgccccag tcggtcgcca caaactccac gttcatgccg
      841 atcttcttca gcaggtcatt ggtgacatcg ccaaaccgac cgttgtgagg tccgtggggc
      901 tgatcagcac ggccttctca cccttgtagc cggaagcagc gagcatttcc cgcgccttgg
      961 cgatattcgc ctgcatggct tcccctccaa cctccacgcc atagcggcta ccgcagggga
     1021 aaaccgattt gcagactgta taggcgctgg catccccagc ggtaacggac gccatgtaat
     1081 catcctggtt tacagccatc atcaccgcgc gacgtactgc gacgttgttg aacggcggat
     1141 gcaaatggtt gaagcgcagc accccattgt agccggtggg gttgtggaaa ccgatttcaa
     1201 tagcagggtt tttcttcagc aacgggacga gatccggctg cacctgctca taccagtcca
     1261 cttcaccgct ttgcagcgcg gcagcggcag tggcggagtc aggaataacc cgccattcga
     1321 tccggtcgaa atgcgcaacc ttgccgcctg acatccattc cgccttttcc tggcgcggca
     1381 cataatcgtc gaaacgggca tagccgacat gggcgccgga attgtactcg tccggcagga
     1441 agcggtaggg gccggagccg atgtactcgg tgatctgctt gaacgggtca gtcttggcga
     1501 cgtgttcagg catcataaag gcgacggaag cgccgccgcg cgcgattgct tccaagaaga
     1561 ctgcaatcgg ctgcttcaag gtaatcttga tggtgcggtc gtcaactgcg acgcagtcat
     1621 cgacatattg ccagattgtc tggccgatgg tctcccgccg tgcccatcgg accagactgg
     1681 gtgcgcaatc ctgcgcccgc accggttcac cattatggaa tttcagaccg ttgcgcagct
     1741 tgatcatgta ggtacggcta tcatcggaca gcgtataccc ttccgccatc tgcggcttga
     1801 cttccaactt ggaggttacg gcgaatagca ggtcgtagat cgcccagccg tgatgtaccg
     1861 tgggttgcgc tgtggtgaaa atcggatcca gcagagtcag attggcctgc ggcacgaata
     1921 ccagcgtgcg ggcgctggcg ggctgggcga tggcgaactt ggctggcacg aacggcgcgg
     1981 cgacggcacc ttgcagcaag gtcctgcgct tcatggtgtc tgctccctgt cgattttagc
     2041 cggaccttgg cggaccagca ggcttattgg ggacactata tacgatgatc gtcgggtcga
     2101 ggaacaccag gggatagacc ggctccagcg ggcgcctccg ccacgccagc aggtcctcgt
     2161 gcacggcgtc cgtcatccgg ctgatcaggt ccggcgatac ctccacctgg tccatctgca
     2221 gcagggccgc ggccaagacc cgctatctc
//
LOCUS       DASWQN010000045         4811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_4263, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000045 DASWQN010000000
VERSION     DASWQN010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4263"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VGC82_00725"
     CDS             complement(<1..1162)
                     /locus_tag="VGC82_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421828.1"
                     /translation="MTAPSIAVAQSVPVRSGALDRVWWLALTGLCVVLLAPLLVVDVP
                     PLLDYPNHLARVFVLASLPHDEVLGRFYASNWSIIPNVALDLVAPPLIRVLPVHVVGR
                     MLIAVAVLLPVFGAVAYNTAIGGRWWGFGVGLVAYNSCQLYGFLNFEIALGVALLLAA
                     AWLRWREDRPARAILLAVPGALVLFACHLMGLVFFGALIGGAELWRLCCERTAACVIR
                     RSAALALVFAAPCALYTASALERLGGDAQYLTFGEKLLQLVTTFVNYVLPLDMATAAV
                     AIGVPAICLLVRKGRLPGSAAAAAALLLIAFLAAPYAWKGTFVLDTRFAIMLGFMLFA
                     GFVPARWSSGFGLAVTAALGLLFVARMAVLTTAWTSHRSDIADLRTALAPVQP"
     gene            1396..2397
                     /locus_tag="VGC82_00730"
     CDS             1396..2397
                     /locus_tag="VGC82_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0421829.1"
                     /translation="MPAIAKGAARDVTIAGPDGWFASYFDAAILASFRKAHPDIRVRY
                     DPAGNAFQLLTLLRGQRANPSVDVVLMEAGVAARATAEGLFDPLTRDTMPVMKDLIPQ
                     AILPAVAGPAMILDSLALGYDPAQVSKVPKSWRNLWDTAYGRRIALQTPPDPLGLAIT
                     AVAAALFGSGTPRNSLDIAMNALTQLAPRVVLWDPVPDIYTAIVAGDADIGPCWNARA
                     QSPAAQTRGRFAAETPSEGSPFLATTVNLVKHSAQTDAAQALIAWLLGTQAQRLLTET
                     MFFAPVNARADIPTASLARAGATPEMADRRMEMDWVAVTAIRDQITAAWRARKLASH"
     gene            2406..3356
                     /gene="thiL"
                     /locus_tag="VGC82_00735"
     CDS             2406..3356
                     /gene="thiL"
                     /locus_tag="VGC82_00735"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY0421830.1"
                     /translation="MVLPAEFSLIARHFRPLAGPGALELRDDAALLTPPPNRQLVLTA
                     DAMVAGVHFLPGDPPDLIGRKLLRVNLSDLAAKGAVPLSYLMTVSAPTQTPEAWFAGF
                     ASGLAQDQATYGVTLLGGDTTSTPGPISLSLTIIGHVAPGTAVHRFGARDGDGIWVTG
                     TIGDGALGLAVALGKLADPSGFLLDRYRLPQPRVGLALAGVASAAMDVSDGLVQDLGH
                     LCRASGLAADIDVANVPLSQAARSAGPEWLMTCLTGGDDYELLLAVPPDRSDALRMAA
                     QRVGIAVTRVGHFHSGPPEVMVRQPDGEPLALKAGGWSHF"
     gene            3435..4604
                     /locus_tag="VGC82_00740"
     CDS             3435..4604
                     /locus_tag="VGC82_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0421831.1"
                     /translation="MNKNVWLLFACQALMNAVMSGQTVMASLVGYKLSGTALNTLPMA
                     IQMVSVMSASVVAGYAFPRFGRRGGFWLGCAIMMCGSLTFALGIYTGNFVVYCLGAIP
                     AGLGFGISQHLRFAAAEVADPHAKARAISLVLAGGVLAAIVGPEMVKRTNALIPDYVF
                     LATYLYLPMLPVITAILLSFVRVPPAQKAVGVPVPFRTIFARPAFVTAVVSSLVGYGT
                     MNLIMASTPLQMLLCGYGVAASADVIRAHSIAMFAPGFVTGRLIQKFGVHRIIMIGGL
                     LTLSCVAVNIYEPPLFATFMVALALLGVGWNFMFVGGTTILTTAYAPEERVRIQATHD
                     FIVFGSVALTALTSGAVQATQGWNFLNLTVVPPVLIAFVVAGWHWIARARTAVVA"
     gene            complement(4629..>4811)
                     /locus_tag="VGC82_00745"
     CDS             complement(4629..>4811)
                     /locus_tag="VGC82_00745"
                     /inference="COORDINATES: protein motif:HMM:NF045247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421832.1"
                     /translation="LTGWYPGGKLEMDEAQRSRKTTKFGSVKWVFPKERMTEMRAALQ
                     ASLACRLPMTRVLYWT"
BASE COUNT          822 a         1582 c         1579 g          828 t
ORIGIN      
        1 ccggctgcac cggcgccagg gccgtgcgca ggtcggcgat gtcgctccgg tggctggtcc
       61 aggcggtcgt cagcaccgcc atccgggcca cgaacagcag cccaagggcg gcggttacgg
      121 cgaggccgaa gccgctggac cagcgcgcgg gcacaaaccc ggcgaacagc atgaagccca
      181 acatgatggc gaagcgcgtg tccagaacga aagtcccctt ccacgcatac ggtgcggcga
      241 ggaatgcgat cagcagcagc gccgccgccg cggcggcaga gccgggcaat cttcccttgc
      301 gcaccagcag gcagatcgcc ggcacgccga tcgccacggc cgcggttgcc atatccagcg
      361 gcaggacata gttgacgaac gtcgtcacaa gctggagcag tttctcgccg aacgtgaggt
      421 actgggcatc gccacccagc cgttccaggg ccgaggcggt gtacaatgcg caaggcgcgg
      481 caaataccag cgccagtgcc gcgctgcggc gaatgacgca cgcggcggtt cgttcgcagc
      541 acaggcgcca cagttcggcg ccgccgatca gcgccccgaa gaataccagg cccatcaaat
      601 ggcaggcgaa caggaccaaa gcaccgggaa cggccagcag gatcgcccgc gccggccgat
      661 cctcccgcca gcgcagccat gcggcggcga gcagaagggc gaccccgagc gcgatctcga
      721 aattcagaaa gccgtagagc tggcaactgt tgtaggcaac caggccaaca ccgaaccccc
      781 accatcttcc gccgatcgcg gtgttgtagg cgacggctcc gaacaccggg agcaggacgg
      841 cgaccgcgat cagcatccgg cccaccacat gaacgggcag cacgcggatc agcggtggcg
      901 ccaccaggtc aagcgccacg tttggaatga tcgaccagtt cgacgcgtag aagcggccca
      961 ggacctcatc gtggggcaac gaggccagca cgaacacccg agccagatgg ttgggatagt
     1021 ccagcagcgg cggcacatcc accaccagca acggcgccag cagcacgacg cacaggccgg
     1081 tcagggccag ccaccagacc cgatccaggg cgccggaccg aaccggaacc gattgcgcca
     1141 cggcgatgct gggtgccgtc attcgttagc cgggtccgca agctggtgat atacagcgcg
     1201 gctcaatagc ggctcgcggc ggcaaccgat acgtcgaatc tatttcaacg tcgagatttt
     1261 cgcttcgagc aagcacttcg gcaacaggac actatgcgcg tctggacgcc atggcttgca
     1321 tcaccgccgt tgccgctgcc tttgcctggt ccgcaactcc agcgccgggc ttttgccgct
     1381 ggggcgctcc tggcaatgcc ggctatcgcc aaaggggcgg ctcgtgatgt caccatcgcc
     1441 ggcccggatg gctggttcgc gagctatttc gacgcggcaa tcctcgcctc cttccgcaag
     1501 gcccatcccg acatccgcgt ccgctatgac ccagccggca acgcgttcca gttgctgacc
     1561 ctgctgcgtg gccaaagggc aaatccatcg gtcgatgtgg tgttgatgga ggcgggtgtg
     1621 gccgcgcggg cgaccgccga aggcttgttc gatccactga cccgcgacac catgccggtc
     1681 atgaaggacc tgatcccgca ggcgatcctg cccgccgtcg ccggacccgc gatgatcctg
     1741 gacagcctgg cgctgggcta cgatcccgcg caagtgtcca aggttcccaa gtcctggcgc
     1801 aacctttggg acaccgccta tggacgccgg atcgccctgc agaccccgcc cgatccgctg
     1861 ggcctggcga taaccgcggt ggcagccgcg ttgttcggta gtggcacccc gcgaaactcg
     1921 ctggacatcg cgatgaacgc tttgacccaa ctggctccgc gcgtcgtgct atgggatccg
     1981 gttccggaca tttacaccgc cattgtggcc ggcgacgccg acatcggtcc ctgctggaat
     2041 gcccgcgccc aaagcccggc ggcgcaaaca cggggacgct tcgcggcgga aacccccagc
     2101 gaaggaagcc cgttccttgc aacaaccgtc aacctggtaa agcacagcgc gcaaaccgat
     2161 gccgcgcagg ccttgatcgc ttggttgctc ggtacgcaag cccagcgtct gctgaccgag
     2221 acgatgttct tcgcgccggt caacgccagg gcggatattc cgactgcgtc gctggcgcga
     2281 gccggcgcca cgccggaaat ggcggaccgg cggatggaga tggactgggt tgccgtgacc
     2341 gcgatccggg atcagatcac cgccgcctgg cgggcccgca agctcgcgag ccattgaagg
     2401 aagacatggt tctaccggca gaattctcgc tgattgcccg gcactttcgc ccgctggccg
     2461 gtccgggcgc attggaactg cgggacgatg ccgccctgct gacgcctccg ccgaaccggc
     2521 aactggtcct gaccgcggac gcgatggtcg ccggggtgca tttcctgccc ggcgatccgc
     2581 ccgacctgat cggccgcaaa ctgctgcggg tcaacctttc cgacctcgcg gcaaaagggg
     2641 cggtgccgtt gagctacctg atgacggtgt ccgctccgac gcaaacaccc gaggcctggt
     2701 tcgccgggtt cgcctccgga ctggcgcagg atcaggcgac ctacggcgtg acgcttctgg
     2761 gcggcgacac gacctcgacg cccgggccga tttcgctgtc gctgacgatc atcggacatg
     2821 tcgcgccagg caccgccgtg catcgctttg gcgcccggga tggcgacggg atctgggtca
     2881 ccggcacgat cggcgacggt gcactcgggt tggccgtcgc gctgggaaag cttgccgacc
     2941 cgagcgggtt cctgctggac cgttaccgct tgccgcagcc gcgggtggga ttggcgttgg
     3001 ccggcgtcgc ctccgccgca atggatgtat cggacggcct ggtgcaggac ctcggccatc
     3061 tctgccgagc cagcgggctg gccgcggata tcgacgtggc caatgtcccc ttgtcccaag
     3121 ccgctcggtc tgccgggccg gagtggctga tgacttgcct gaccggtggc gacgactacg
     3181 agcttctcct tgcggtgccg ccggaccgat ccgacgcgtt gagaatggct gctcagcgtg
     3241 tcggcatcgc tgtcacgcgg gtcggacact tccattccgg tccgccggag gtcatggtgc
     3301 ggcaaccgga tggagaaccc cttgctctga aagcgggtgg ctggagtcat ttttagcgcg
     3361 aggcttgtgt tgggcagaat gtcccggcgg attggtcgtg cggtcaaaca aatacgaatg
     3421 ggcgtcctgg aaaaatgaac aagaatgtct ggttgctgtt cgcctgtcag gcgctgatga
     3481 acgctgtcat gtcgggccag acggtcatgg cgtcactggt cggctacaaa ctgtccggca
     3541 ccgcgttgaa cacgctgccg atggcgatcc agatggtctc ggtcatgagt gcgtcggttg
     3601 tcgccggcta tgccttcccc aggttcggcc ggcgcggcgg attctggctg ggttgcgcga
     3661 tcatgatgtg cggcagcctg accttcgcct tgggcatcta caccggcaat ttcgtggtct
     3721 attgcctggg tgcgatcccg gcgggtctcg gcttcggtat ctcccagcat ctacgcttcg
     3781 ccgcggccga ggtcgccgac ccgcacgcca aggctcgggc gatttcgctg gtgctggcag
     3841 gcggcgtgct ggcggcgatc gtcgggccgg agatggtcaa gcgcaccaac gcgctgatcc
     3901 ccgactatgt gttcctggca acctatctgt atctgccgat gctgccggtc ataacggcga
     3961 tcctgctgag tttcgtgcgg gtgcctccgg cgcagaaggc tgtcggcgtc ccggtcccct
     4021 tccgcaccat ctttgcccgc ccggcgttcg tcaccgcggt cgtcagctcg ctggtgggct
     4081 acgggacgat gaacctgatc atggcctcga ccccactgca gatgctgctt tgcggctacg
     4141 gcgtggcagc gagcgccgat gtgatccggg cgcattcgat cgcgatgttc gcgccagggt
     4201 tcgtgaccgg gcgactgatc cagaaattcg gcgtgcaccg gatcatcatg atcggcggcc
     4261 tgctgacgct gagctgcgtg gcggtcaaca tctacgaacc gccgctgttc gccaccttta
     4321 tggtggcgct ggcgctgctg ggggtgggat ggaacttcat gttcgtcggc ggaacgacga
     4381 ttctgaccac cgcctatgcg ccggaggaac gggtccgcat ccaggcgacg catgatttta
     4441 tcgtgttcgg cagtgtcgcg ctgacggcgc tgacatccgg cgcggtgcaa gcgacgcaag
     4501 gatggaattt cctcaatctg accgtggttc cgccggtgct gatcgccttc gtcgttgccg
     4561 gttggcactg gatcgcccgg gcgcgcacgg cggtcgtggc ttgacgggga acccatacgg
     4621 tagcggcctt aggtccagta caggactcgc gtcataggca gccggcaagc aagcgaggct
     4681 tggagggctg cacgcatctc ggtcatccgt tccttcggga acacccactt caccgaaccg
     4741 aactttgtcg tcttgcgtga ccgctgagcc tcatccatct cgagcttgcc gccgggatac
     4801 cagccggtga g
//
LOCUS       DASWQN010000046         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_663930, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000046 DASWQN010000000
VERSION     DASWQN010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663930"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VGC82_00750"
     CDS             complement(<1..1236)
                     /locus_tag="VGC82_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY0421833.1"
                     /translation="MTAVGHDTLKTRRTLNVEGKGYEYFSLPEAAKTLGDISRLPVSL
                     KILLENVLRFEDGRSYKVDDAKAIAGWLEKASSSREVPFKPARILLQDFTGVPAVVDL
                     AAMRDGITRLGGDPKKVNPLVPVDLVIDHSVQVDAFARADALTRNVDIEFERNGERYK
                     FLRWGQDAFDNFRVVPPGTGICHQVNLEYLGQCVWTSTNTGTTFAYPDTLYGADSHTT
                     MVNGLGILGWGVGGIEAEAAMLGQPIAMLIPDVIGFRLTGKMPEGTTATDLVLTVTQM
                     LRRKGVVGKFVEFFGPGLDDLGLPDRATIGNMAPEYGATCGFFPVDKVALDYLRLSGR
                     DTHRIALVEAYLKAQGMFREPGTPDPTFTDTVELDLSTVEPSVAGPKRPQDRVLLKDV
                     ASSFKADLTKGLGVPANEAG"
     gene            1394..1987
                     /gene="ccmA"
                     /locus_tag="VGC82_00755"
     CDS             1394..1987
                     /gene="ccmA"
                     /locus_tag="VGC82_00755"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEY0421834.1"
                     /translation="MLVAEDAAVLRGERLVFRDLSLTVPAGGALVLAGPNGSGKSTLL
                     RLLSGLVRPAAGRILWDGGDALLDLAAHGRRVAYLGHQDAVKPGLTVAENLEFAAAVS
                     GRPIDPALTAVGLEALAELPARMLSAGQKRRLALSRLLLSAAPLWLLDEPTLGLDTAS
                     IHRFGDVLAAHREAGGMVVAATHVPLPLPNAAELRLG"
     gene            1987..2652
                     /gene="ccmB"
                     /locus_tag="VGC82_00760"
     CDS             1987..2652
                     /gene="ccmB"
                     /locus_tag="VGC82_00760"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY0421835.1"
                     /translation="MRVFRAVLVRDLRLSLRHGADTLAALLFFMVTASLFPLAIGPAP
                     DTLGRIAPGIVWVCALLAALLPLDRLFGADLEDGSLDLLLLSGLPAFAVAAAKTLAHW
                     LVTGVPLLLAAGPIAIMLRMPGDAIPALLLGLLPGGFLLSLFGTMVASIVLGARRGGV
                     LLPLLVLPLVTPVLIFGAAAADAAAGGFSARPHLLLLGAMLAAAAPLCPLAAGAGLRA
                     AVE"
     gene            complement(2663..>3523)
                     /locus_tag="VGC82_00765"
     CDS             complement(2663..>3523)
                     /locus_tag="VGC82_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010669017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY0421836.1"
                     /translation="ALRQLEGALGALYQDWGDRLRGILAYQEALIDFPDEDLPPEVEG
                     QLLTALRSVRAEIATHLNDAGRGEKLREGLFFAITGAPNVGKSTLINALAERDVAIVS
                     EIPGTTRDAVEARIILGGVPVTLVDTAGLRETTDSIEAEGVRRALARARDADLAMTVI
                     EAGWPAPDNPGHLLIANKADLARPGPDGALRVSAKTGLGLPELRAHLATAARQMTESS
                     GPPPLTRARHRAALLTAVEHLDAADQADLPELRGEDLRLAMRALGRITGHVGVEDILD
                     TVFSRFCIGK"
BASE COUNT          533 a         1171 c         1158 g          661 t
ORIGIN      
        1 ccctgcctcg tttgccggca cgcccaggcc cttcgtcaga tccgccttga aggaagaagc
       61 aacgtctttc agcagcaccc gatcctgcgg ccgcttcggc cccgccacac tcggttccac
      121 ggtggacaga tcaagttcca cggtatcggt gaatgtcggg tcgggtgtcc cgggctcccg
      181 gaacatgccc tgggccttca ggtaggcctc aaccaacgcg atgcggtggg tatcgcggcc
      241 ggacaggcgc agatagtcca gtgcgacctt gtccactggg aagaatccgc aagtagcgcc
      301 atactcggga gccatgttgc caatggtggc acgatctggc agccccagat cgtccagccc
      361 cggcccgaag aattcgacga atttcccgac gacacccttt cggcgcagca tttgcgtcac
      421 cgtcagcacc agatcggtgg cggtcgtccc ttcaggcatt ttgccggtca gacggaagcc
      481 gatcacgtcc gggatcagca tggcgatggg ctgccccaac atggcagcct cggcctcgat
      541 cccgccgacg ccccacccga ggatacccag gccattcacc atcgtcgtgt ggctgtcggc
      601 gccatacagc gtgtccggat aggcgaatgt cgtgccggta ttggtggatg tccacacgca
      661 ctgaccgagg tattccagat ttacctgatg gcagatgccg gtacccgggg gcacgacccg
      721 gaagttatcg aacgcgtcct ggccccagcg caggaatttg taacgctcgc cgttacgctc
      781 gaattcgata tcgacgttcc gggtcagcgc atcggcgcgg gcgaacgcgt ccacctgaac
      841 cgagtggtcg atgaccaggt caaccggcac cagcgggttt accttcttcg gatcgccgcc
      901 cagccgggtg atgccgtcgc gcatggcggc caaatcgacg accgccggca caccggtaaa
      961 atcctgcaac aggatgcgcg cgggcttgaa cggcacctct ctcgaagacg acgccttctc
     1021 cagccagccg gcgatcgcct tggcgtcatc caccttgtag ctacggccgt cttcaaagcg
     1081 cagcacgttt tccagcagga tcttcaggct gaccggcaag cgggatatgt cgccgagggt
     1141 cttcgccgct tccggcagcg agaaatattc gtatccctta ccctcaacgt ttaatgtgcg
     1201 gcgagtcttg agggtatcgt gcccgactgc ggtcatgcgc ccgtttcgcc ccggttcgcg
     1261 gggtgccttc cgttgaggcg gcgtaaggtg gagacgccgc ggatgaaatg ttcacgatgg
     1321 cgggctttaa ccacaccggc cggtagccgt ccatccaggg ctgccgcgac gcagatccga
     1381 tcggaggaca aacgtgcttg tggctgagga cgcggcggta cttcgaggcg agcggctggt
     1441 gtttcgcgac ttgtcgctga ccgtgccggc cggtggcgcg ctggtgctcg ccggaccgaa
     1501 cggatcgggc aaatcaaccc tgctgcgcct gctgtccggc ttggtgcggc cagcagcggg
     1561 gcggatcctg tgggacggtg gcgacgcgct tctcgacctc gcggctcatg gccggcgcgt
     1621 ggcttacctt ggtcaccagg acgcggttaa gccgggcctg accgtcgccg agaacctgga
     1681 gttcgccgcc gccgtgtccg gccgcccgat cgaccccgcc ctgacggcgg tgggactgga
     1741 ggcactggcg gaattgcccg cccgcatgtt gtcggcggga cagaagcggc ggctggcgct
     1801 atcgcggctg ttgctgtccg ccgcgccgct ctggctgctg gatgagccga cgctcggtct
     1861 cgacaccgcc tcgatacatc ggttcggcga tgtgctggcc gcacaccggg aagcgggggg
     1921 aatggtcgtg gcggcgaccc atgtgccgct gccgttgccg aacgcggccg aactgcggct
     1981 gggttgatgc gcgtattccg ggcggtcctg gtccgcgact tgcgcctgtc gttgcgccat
     2041 ggtgcggaca cgctggcagc actgttgttc ttcatggtga cggcgtcgtt gttccctttg
     2101 gcgatcggac ccgctcccga tacgctggga cggatcgcgc ccggcatcgt ctgggtgtgt
     2161 gcgctgctcg cggctttgct gccgctggac cggctgtttg gcgccgacct ggaggatggg
     2221 tcgctggacc tgttgctgtt gtcagggctg ccagcttttg cggtcgccgc ggcaaagacc
     2281 ctggcgcact ggctggtcac cggcgtgccg ctgcttctcg ccgccggccc cattgccatc
     2341 atgctgcgca tgccaggcga tgccatccct gccctgttgc tcggtctgct tcccggcggc
     2401 ttcctgttgt cgctgttcgg caccatggtc gcctcgatcg tgctgggtgc tcgccggggc
     2461 ggcgtgctgc ttccgctgct ggtcctgccg ctggtgactc cggtgttgat cttcggcgcc
     2521 gccgccgccg atgccgccgc gggcgggttc tccgcgcgac cccatctgct gttgttgggc
     2581 gcaatgctgg ccgcggcggc gccattatgt cccctggcgg cgggcgctgg attgcgagcg
     2641 gcggtcgagt aaccgaccag cgttacttcc cgatgcagaa gcgcgaaaac accgtgtcga
     2701 ggatatcttc caccccgaca tggccggtta tgcgtcccag ggcccgcatc gccagccgca
     2761 gatcctcgcc acgcagttcc ggcagatcag cctgatcggc ggcatccaga tgctccaccg
     2821 ccgtcagcaa cgccgcccga tggcgggccc gggtcagtgg aggcgggccg ctcgactccg
     2881 tcatctgccg agcagcggtg gcaagatggg cgcgaagttc gggcaggccc aggccggtct
     2941 tcgcgctgac gcggagcgcc ccatccggac ccgggcgagc aagatcggcc ttgttggcga
     3001 tcagcaggtg gccggggttg tcaggcgccg gccagccggc ctcgatcacg gtcatcgcca
     3061 gatccgcatc ccgcgcccga gcaagggcac ggcgcacgcc ttcggcctcg atgctgtccg
     3121 tggtttcccg caaaccggcg gtatcgacca gcgtaaccgg cacgcccccc agtatgatgc
     3181 gggcttccac tgcatcccgg gtagtgccgg gaatttcgga aacaatggcg acgtcgcgtt
     3241 cggccagtgc gttgatcaag gtcgatttgc cgacattcgg cgcgccggtg atggcgaaga
     3301 acaggccctc gcgcagtttc tcgccgcggc cggcatcatt gaggtgagtg gcgatttccg
     3361 cccgaaccga gcgcagtgcg gtcagaagct gaccttcgac ctccggcggc agatcttcgt
     3421 ccggaaaatc gatcaacgct tcctgatacg ccaggatccc tcgtaaccga tctccccagt
     3481 cctgatacaa cgcgccaagc gcgccctcga gctggcgcag tgc
//
LOCUS       DASWQN010000047         1880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1455731, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000047 DASWQN010000000
VERSION     DASWQN010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455731"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VGC82_00770"
     CDS             <1..479
                     /locus_tag="VGC82_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY0421837.1"
                     /translation="RERRRKRGACIDPHGFDAGKLTKGKKRHILVDTQGLMLHSLVTS
                     AGVQDHDGGILLLSTLFGLFPFLKKLFADSAYEGPIFRTALAKIFPGLEVEIVKRSDQ
                     TKGFVVLPKRWIVERSLAWLNRCRRLAKDWENFNRSACAFLRLASIRLMLRKLCNG"
     gene            complement(1102..>1880)
                     /locus_tag="VGC82_00775"
     CDS             complement(1102..>1880)
                     /locus_tag="VGC82_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0421838.1"
                     /translation="QGIDYILGVRERSTAEVRSTVIDDDGVAVPLTIPRQKGETDLAI
                     KDVVVAGRRYVLCRNAEQARKDAETRTALLASLERKLTQGDKALVGNAGYRRFLADPA
                     GDGFTVDAAKVEADARFDGLFVLRTNTKLTALQVVLRYRNLLAVEEGFLAAKTLLATR
                     PIFHRTDAAIRGHIFCTFLALVLRKELMDRLAARHIAMPHWQQVVDDLADLSEIEVEQ
                     DGRRALLRTAPGPTIDALCRAVAVTLPPVFQELPRAEAAS"
BASE COUNT          357 a          557 c          577 g          389 t
ORIGIN      
        1 agcgtgaaag gcggcgaaaa aggggggcct gcatcgatcc gcacggcttt gatgcgggca
       61 aactgaccaa gggcaagaaa cggcatattc tggttgatac gcagggcctt atgctgcaca
      121 gtctggtcac gtctgccggg gttcaggatc acgatggcgg cattttgctg ttatcgacgt
      181 tgtttggcct gtttccgttc ctgaagaaat tgttcgccga tagtgcctat gaagggccga
      241 tcttccgcac cgctctggcc aaaatcttcc ccggcctgga ggtcgagatc gtcaaacgct
      301 ccgatcagac caagggcttc gttgttctgc ccaaacgctg gatcgttgaa cgctcgcttg
      361 catggctcaa ccgctgccga cgcctcgcca aggattggga gaatttcaac cgcagcgcct
      421 gcgcttttct gcgcctcgct tcaatccgcc tcatgctgcg aaaactctgc aatggttgag
      481 attctttcgg aacggacact gaaattggcc ggtgcgtaat tggttcgcac gcggcattca
      541 tcgtcgcgga agatcatgtc cagcacccag tgcaggctgt ggtgggtagg ccggcgggat
      601 ttcacctccg gcctctcgca gaaccctgcg tgagactctc acctcacacg gctcccatca
      661 ggcgaacgcg ctttctgcca atgtgcgaag atatacgggt cttccgtgag atggtctcga
      721 ggaagatgct tgcccgtgtc ctatgaccag cgaggcgttt gtacttccgc ctggcccatg
      781 ccaccaggac aggttgacat ggcggaacat ctgatacagc gctgagggac tgtagcgccc
      841 gtaatactcg atccagcctg gtatgactgg attgatccca ttggcgacgt ctttcagcgt
      901 gatctgggtt ctgttgcgca agttggtctt ccggatcgtc tcccgcatgg cggtgagcac
      961 cgcctggctc agcgcgggcg tgaagctcca gaacaggctg ccctgacctg acccaacttt
     1021 cccaccagtt cgaaaatata gtggttagaa cgaggcaata gaggccctgt tatgatttag
     1081 gcaccacagt tccggggggt atcaggacgc tgcttcagcg cgcggaagtt cctgaaacac
     1141 cggcggcagg gtcacagcga cagcgcggca aagcgcgtca attgtcggtc cgggcgcggt
     1201 gcgcagcaac gcgcgccgtc cgtcctgctc gacctcgatc tcactgagat cggcgagatc
     1261 gtcgacgacc tgctgccagt gcggcatggc gatatgacgg gctgccagcc ggtccatcag
     1321 ttctttgcgc agcaccaggg cgagaaaggt gcagaagata tggccgcgga tagcggcatc
     1381 ggttcggtga aagatcggcc ttgtagccag cagcgtcttg gcagcgagga agccttcctc
     1441 caccgccagc aagttgcgat accgcagtac cacctgcaag gctgtcagct tggtgttggt
     1501 gcgcagcacg aacaggccat cgaagcgagc atcggcctcg actttcgcgg catcgacggt
     1561 gaatccgtca ccggcgggat cagccaggaa gcggcggtac ccggcattgc caacgagagc
     1621 cttgtcgccc tgtgtcagct tgcgttcgag actggccagc aatgcggtcc gtgtctcggc
     1681 gtccttgcgg gcctgttcgg cgttgcggca cagcacgtag cggcgcccgg caacaacgac
     1741 atccttgatg gcgaggtcgg tctcgccctt ctggcgcggg atggtcagcg gcaccgccac
     1801 gccgtcgtca tcgatcaccg tgctgcgcac ctcggcagtt gagcgctcgc gcacaccgag
     1861 gatgtagtcg atgccctgcg
//
LOCUS       DASWQN010000048         1786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_400473, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000048 DASWQN010000000
VERSION     DASWQN010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400473"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VGC82_00780"
     CDS             complement(<1..184)
                     /locus_tag="VGC82_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421839.1"
                     /translation="MTASGFGRAHLVAGGFPPGSSAGHDHDFARLRLLGLLSEQDVPA
                     SVANDFSDIEKWLPVSR"
     gene            407..1177
                     /locus_tag="VGC82_00785"
     CDS             407..1177
                     /locus_tag="VGC82_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0421840.1"
                     /translation="MSDVVSPRPFEGRTALVTGSGQNIGRAIALAFARGGANVVINGH
                     RNLAAIEHVADEARGLGVRAIAVLADVGDPEAVQKMVDRAVEAFGSVDIAVSNVSTRL
                     HTKFLDISIEEWQRILNSNLSASFYLARATLPLMQARGWGRIIHISGRDGFAVIPNRA
                     HNVTCKAGVFALSKAIAVEFGAFGITANTVAPGIIDTTRDLTQYPHFRDGYGDRIQQI
                     PVRRIGHVDEIAEACTYLAGAGFVTGQVLHVNGGDGLF"
     gene            complement(1214..1393)
                     /locus_tag="VGC82_00790"
     CDS             complement(1214..1393)
                     /locus_tag="VGC82_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421841.1"
                     /translation="MPGIGSKVGVKHIMPEQEATYRPRQLSLECCNGNMWGSFAETEG
                     LPREPPASAKIRLVA"
BASE COUNT          310 a          572 c          580 g          324 t
ORIGIN      
        1 accggctaac cggcagccat ttctcgatgt cggaaaaatc gttggcgacc gaggcgggaa
       61 cgtcttgttc ggacagcagc ccaagcagcc tcaggcgggc gaagtcgtgg tcgtgcccgg
      121 cgcttgaacc gggagggaaa ccaccggcga cgagatgcgc gcggccgaat cctgatgctg
      181 tcatcggatc actccccctc ggttcggcgc gggcaatcgc ccgtgctcgt ttgtctgtgc
      241 cccatcgtag cccgcgccgg ccgcgcatgc caaaccgccg cggccacaaa ccaggagatc
      301 gtgtgcctgg cgacggtgcg cggcactatc gcgccggcgt ttacagcatg cgcgtcgaac
      361 gccatcctga ggctcttgat gcacgccagt ccggaggttc cacgcgatgt ccgatgtcgt
      421 ctcgccccgg ccgttcgaag gccgcaccgc gcttgtcacc ggctcgggac aaaatatcgg
      481 gcgggccatc gcactggcat tcgcgcgcgg aggtgcgaac gtggtcatca acggccaccg
      541 gaacctggcc gcgatcgagc acgttgcgga cgaggcgcgc ggcctgggcg tacgggcgat
      601 cgcggtgctg gccgatgtcg gtgatcccga agcggtgcaa aagatggtgg accgcgcggt
      661 tgaggcgttc ggcagcgtcg atatcgcggt atcgaacgtt tcgacccggc tgcataccaa
      721 attcctcgac atctcgatcg aggaatggca gcgcatcctc aattccaacc tcagcgcgtc
      781 gttttatctg gctcgggcga cgctgccgct gatgcaggct cggggttggg ggcgaatcat
      841 ccacatctcc ggccgcgacg gcttcgcggt gattccaaac cgagcgcaca acgtgacctg
      901 caaggcaggc gttttcgcgc tgtcgaaggc gatcgcggtg gagttcggcg cgttcggcat
      961 taccgcgaat accgtggcac cgggcatcat cgacacgacg cgcgatctga cgcagtaccc
     1021 gcatttccgc gacggctacg gcgaccggat acagcaaatt ccggtgcggc gcattggcca
     1081 tgtggatgag atcgcggagg cgtgcaccta cctggcgggt gccggcttcg tcaccggcca
     1141 ggtgctgcac gtcaacggcg gtgacgggct gttctgatga accggctgcc ccacgggttc
     1201 cggcgccgac cggctacgca accagacgta tcttcgcgct ggccggcggt tcgcgcggca
     1261 gcccttcggt ctcggcaaac gacccccaca tgttgccgtt gcagcattcc agcgaaagct
     1321 gacgcggacg gtaggttgcc tcctgctccg gcatgatgtg tttcacgccg accttcgaac
     1381 caatcccggg catgtgcttg tcgcaacaag ccgtattcac tggcacccgg tccggttgac
     1441 aatcggtgcc gtcgctgcgt tgcgggcttc gtatgcggga ccccaacccg gttccaatcg
     1501 ttgctgattt cccccgtgac tgcataaatc caatgtccgc acccccgatt atcaattccg
     1561 ctgaataggc ccatctaccg tgcaacttgg ttgggcagag cggatacatg gaacaggcaa
     1621 tgacggcgga acgggccatg gacggcgggc tacggggcat tcgcatccga accggcgcgg
     1681 ctcgccacaa gggactgttg aagcagatcg cgtttggggc gtttggcgtg ctgggcctgg
     1741 ccgcggccgc ccattacggc acggactact ggcgcaacgg ccgatt
//
LOCUS       DASWQN010000049         2560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1060173, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000049 DASWQN010000000
VERSION     DASWQN010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060173"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VGC82_00795"
     CDS             complement(<1..838)
                     /locus_tag="VGC82_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEY0421842.1"
                     /translation="MLASLVALLGAATIAVPLTRRFGLGSVLGYLLAGVAIGPSGLRL
                     VSDIDQIAGIASLGVVMLLFLIGLELRPRRLWVMRQAVFGLGSAQVVLSGVALAILAH
                     FTGVPWPGAIVLGAGLAMSSTAIVLPMLAEAELLQSRAGRDGFAVLLFQDLAFIPLVA
                     IVPLLAEHTLPDHVPWHDVARAAVAVAVILVGGRFVIPRLIRAVGGARTREIFTTASL
                     LIVAGTAYIANFAGLSMSLGAFMAGVLLSESEYRHELQADINPFEGLLLGFFFISVGM
                     SAD"
     gene            complement(838..1818)
                     /locus_tag="VGC82_00800"
     CDS             complement(838..1818)
                     /locus_tag="VGC82_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY0421843.1"
                     /translation="MRIHLQNPHNDPLFDFSLTMWEAAAARAPDIGEGHTVSIGATPA
                     DFFSAMREAEALVTDVSVIKARFPCDAPHLKLIFLTNAGLDGLAPFTWLPPGVILLNN
                     RGVHAAKSGEFAIMSILMLANRVPAMVSNQRQGLWRKLWGSVLAGRNLTVVGLGTLGG
                     ATAEHAARFGMRVTGVRANPSPHPFCQHVVGTEALDEILPETEFLVLACPLTDATRGL
                     MNRQRLNCLPSSAGIVNIGRGELLDQDALCDLLDQEHLSGAVLDVFTPEPIPPHHRLW
                     STPNLIISPHTSADDPATYNPRSLDIFLDNLRAWRDQRALPNRFDIARGY"
     gene            1920..2363
                     /locus_tag="VGC82_00805"
     CDS             1920..2363
                     /locus_tag="VGC82_00805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0421844.1"
                     /translation="MTGTPVQIVSRHDMSIIDIDRLEDRLYDHNRRATGSSDGKGLAF
                     VALDEQGVQIGAIAGYSWARMAEIKQLWVDEIERGRGLGRGLLEAAIAEAVVRGCQSI
                     WVMSYDFQAPNFYERLGFRREAELADWPPGHTHIILRRRLQDDAA"
BASE COUNT          519 a          764 c          874 g          403 t
ORIGIN      
        1 gatccgccga catgccgacg gagatgaaga aaaaaccgag cagaagtccc tcgaacggat
       61 tgatgtcggc ctgcagttcg tggcggtatt ccgactcgga cagcagcacc ccggccatga
      121 acgcaccgag cgacatggac aggccagcga agttagcgat gtaggcggtg ccggcaacga
      181 tcagcagcga ggcggtggtg aaaatttcgc gcgtgcgggc gcctccgacg gcgcggatca
      241 gccgcgggat gacaaaccgc ccgcccacca ggatgacggc gacggcgacc gcggctcggg
      301 cgacgtcgtg ccaagggacg tggtcgggca gcgtgtgttc ggccagcaac ggcacgatgg
      361 caacaagcgg gataaacgcc agatcctgga acagcagcac cgcgaatccg tcccgtccgg
      421 ctcgcgattg cagtagctcg gcctccgcca gcatcggcag cacgatggcg gtggaggaca
      481 ttgccagccc ggcgccaagc acaatggcac cgggccatgg gactcccgtg aagtgtgcca
      541 ggatcgccag tgcgaccccg ctcaacacga cctgcgccga tccgagtccg aacacagcct
      601 gacgcatgac ccataatcgg cgagggcgaa gctccagccc gatcagaaac agcagcatga
      661 cgacgccgag ggaggcgatg ccggcaatct ggtcgatgtc gctgacgaga cgaagaccgg
      721 aggggccgat cgccaccccc gccagcagat agccgagcac gctgccgagt ccgaaccgcc
      781 tggtcagcgg gacggcgatg gtcgcggcgc ccagcagggc gacgaggctc gccagcatca
      841 atagccccgg gcgatgtcga agcgattggg aagggcgcgc tgatctcgcc aggcgcgcag
      901 gttgtccagg aaaatgtcga ggcttcgcgg gttataggtc gccggatcgt cggccgacgt
      961 gtgtggactg atgatcaggt tcggggtgga ccacaagcgg tggtgcggcg ggattggctc
     1021 cggcgtgaaa acatccagga cggcgccgga cagatgctcc tgatccagca ggtcgcaaag
     1081 cgcatcctga tcgagcaact cgcctcgacc gatgttcacg atgccggcgc ttgagggcag
     1141 gcagttcagc cgctggcggt tcatcaggcc gcgtgtggcg tcagtcaggg gacaggcgag
     1201 gacgagaaac tccgtctccg gaagtatttc atcgagcgct tccgtgccga cgacatgctg
     1261 gcagaatgga tgtggcgaag ggttggcgcg gacgccggtt acccgcatgc cgaagcgggc
     1321 agcatgttcg gcagtggctc cgcccagtgt tccgaggccg acgaccgtca ggttgcggcc
     1381 ggccaggacc gagccccaca gcttgcgcca cagtccttgc cgctggttgc tgaccatggc
     1441 tggaacgcgg tttgccagca tcaggatcga catgatcgcg aactcgccag acttcgcggc
     1501 atggacgcct cggttgttca gcaggatgac accgggggga agccaggtga acggggcaag
     1561 gccgtcgagg ccggcgttgg tcaggaagat cagcttcagg tgcggcgcgt cgcacgggaa
     1621 gcgggctttg atgacgctga catcggtgac aagcgcctcg gcttcccgca tggcggagaa
     1681 gaaatcggct ggcgtggcgc cgatgctgac agtgtgcccc tccccaatat ccggagcacg
     1741 cgccgcggcg gcttcccaca ttgttaggga gaaatcgaac agtgggtcat tgtgggggtt
     1801 ctgcaaatgg atgcgcatgg agcgaaagtg ccatagcgca tggattatgg cgacaccgat
     1861 ttggttaccg gcctgcgatc gcgacagact gtatgactgc catggccaca ggaggaaaca
     1921 tgaccggaac gccggtgcag atcgtatcgc gccacgacat gtcgatcatc gacatcgacc
     1981 gcctggaaga ccgactctac gatcataacc gccgggcgac gggatcgagc gacggcaaag
     2041 gattggcatt cgtagctctt gacgaacagg gtgtccaaat aggtgcgatt gccggttaca
     2101 gttgggctcg tatggccgag atcaagcagc tctgggtgga tgaaatcgag cgtggccgag
     2161 gtttggggcg aggcctgctg gaggctgcga tcgccgaggc tgtcgttcgt ggctgccaat
     2221 ccatatgggt gatgtcctac gacttccagg cgccgaactt ctacgagcgg ctcggttttc
     2281 gccgcgaggc cgaactggcc gactggcctc cgggccacac ccacatcatc ctgcgccgcc
     2341 gacttcagga cgatgcagcc tgacttgctg gcccttcttg ccgtcgcggc agcatgtcag
     2401 ggtgtgcgaa gtcgccgcga accgcgggtg tcacgggggt tctctgcagc cgaaggcaac
     2461 gatcgctagc cggactccag aagcctcgcc cggcagagac cccaggacga ccgttcaatc
     2521 gaccagcgcg aagaagcggg gattgcacac cattccatgc
//
LOCUS       DASWQN010000050         2920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_268585, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000050 DASWQN010000000
VERSION     DASWQN010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268585"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="VGC82_00810"
     CDS             complement(<1..1201)
                     /locus_tag="VGC82_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY0421845.1"
                     /translation="MRLLVRFFGFLFAAGTVLFLVGVAAVAGLIWHYSKDLPDYSQLQ
                     DYEPPVMTRVHAADGALLGEYSKERRLYLPIQAVPKLVVNAFLAAEDKNFYEHGGIDF
                     TGMARAAVLFAQNYGSNRRPQGASTITQQVAKNFLLTNEVSFTRKIKEALLAMRIERA
                     YSKDKILELYLNEIYLGLGAYGVAAASLVYFDKSVNELTVAEAAYLAALPKAPAALHP
                     VRNRDRAIERRNYVIDRLLENGWIKQADADKARKDQMIVTSRSNAAHTFAGEYFAEEV
                     RRDVFERYGEKKLYEGGLSVRTTLDPKVQVMARKTMAAGLVTFDEQQGWRGAISKIDI
                     SGDWGVKLADSKSLSDISPWRMAVVLETSDQSARIGFQPGRELGGAISKERQTGLITL
                     EGVKWAKA"
     gene            complement(1408..>2920)
                     /locus_tag="VGC82_00815"
     CDS             complement(1408..>2920)
                     /locus_tag="VGC82_00815"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY0421846.1"
                     /translation="WNLLLKVPALHREAESDTTGSFSHQPLLHTWIERPVQGKRRWAA
                     TPCLIRWKCDKVRLTLWSYWGKRLLGGTDSVAGRTNSRVWLGCALMCAAAIVCPDVTA
                     VRAAQSQPPSSAAANFPIASDVRLAGDDKKTRFVLDLDQAVQFRAFTLTDPYRVVVDF
                     PQVSFQMPAGVGTAGRGLIRAFRYGMVMPGGSRMVLDLTGPARIANSYMLDAANGQPP
                     RLVVELEQTDAAAVPSHASEGWPELRPAIADASAATLPAAVISSKPRAPADPRPVVVI
                     DPGHGGIDNGTQAGGESEKTLVLGFALALRDRIEKSGKYRVVMTSTDDTFIPLADRVR
                     IARTQSAALFVSIHADALPRHEGDAQGATIYTLSDRGSDAEAERLAEAENKADAIGGV
                     NLTEEPTEVADILIDLAQRETRTFSNRFARVLMGEMKRTVRMHKHPLKSAGFKVLKAP
                     DIPSVLVELGYVSNKGDLEHLVSESWRSRTVGAMAQAIDAFFAKRLAIAGPTD"
BASE COUNT          535 a          930 c          911 g          544 t
ORIGIN      
        1 ccgccttggc ccatttcacg ccctcaaggg tgataagccc ggtctgccgt tccttgctga
       61 tggcaccgcc cagttcacga ccgggctgga agccgatccg tgcggactgg tcgctggttt
      121 ccagcaccac ggccattcgc cagggcgaga tatcggagag cgatttggag tcggcaagct
      181 tgacgcccca gtcgccggag atgtcgatct tgctgatcgc accgcgccaa ccctgctgtt
      241 cgtcaaaggt gacgaggccc gccgccatgg tcttgcgcgc catcacctgg accttgggat
      301 caagcgtggt cctgaccgac agaccgcctt catagagttt cttttcgccg taacgctcga
      361 acacgtcgcg gcgaacctcc tcggcgaaat attcgccggc gaaggtgtgc gcggcattgc
      421 tccggctggt cacgatcatc tgatccttgc gggccttgtc cgcatcggcc tgcttgatcc
      481 agccattctc aagcagccgg tcgatgacgt aattgcgccg ctcgatggcg cggtcgcggt
      541 tgcgcaccgg atgcagcgcc gccggcgcct tcggcagcgc cgcaaggtag gccgcttcgg
      601 cgaccgtgag ttcgttcacc gacttgtcga aatacaccag cgacgccgca gcgacgccat
      661 acgcgccaag gccgaggtag atttcattga ggtagagctc aaggatcttg tccttggaat
      721 aggcgcgctc gatccgcatc gccagcaagg cttccttgat cttgcgggtg aacgacacct
      781 cgttggtcag caggaagttc ttggcgacct gctgggtgat cgtggacgcg ccctgtgggc
      841 ggcggttgga gccgtaattc tgtgcaaaga gcacggccgc gcgcgccatg ccggtgaaat
      901 cgatgccgcc atgctcgtag aagttcttgt cctcggccgc caggaacgcg ttgacgacga
      961 gcttggggac cgcctggatc ggcagataaa ggcggcgctc cttggaatat tcaccgagca
     1021 gcgcgccgtc cgccgcgtga acgcgggtca tcaccggcgg ctcataatcc tgaagctgcg
     1081 aatagtccgg cagatccttg gaataatgcc agatcagtcc ggccacggcg gcgacgccga
     1141 ccagaaacag gacggttccg gcggcaaaca ggaaaccaaa gaaccgcacc agcaaacgca
     1201 ttagcgaata tccgttccaa cccatgcgcc gtaacgaccg gcgttttttc ctcagcggac
     1261 agtgccgcgg aaccaactgc catcccgcgc gggcctacca tacggcggcc gagagggaat
     1321 ttgcatcgat tccaaagacc tcgcggcggt ttttatagga cggccgctgt ggctaaacta
     1381 gggcttccgc ttcggggagc ccgctgttca gtcggtcggc cccgcaatgg ccagtcgttt
     1441 ggcaaagaag gcgtcgatgg cctgcgccat cgcgccaacg gtgcgcgagc gccagctttc
     1501 cgagaccaga tgttcgaggt cgcccttgtt cgagacataa ccgagctcga ccagcaccga
     1561 cggtatgtcc ggtgccttga gtaccttgaa gccggcggat ttcaggggat gtttgtgcat
     1621 ccgcaccgtg cgcttcatct cgcccatcaa cacccgcgcg aaccggttcg agaaggtccg
     1681 ggtctcgcgc tgggcaaggt cgatcaggat gtcggcgacc tcggtcggct cctcggtgag
     1741 gttgacgccg ccgatggcgt cagccttgtt ctcggcctcg gcgagccttt cggcctccgc
     1801 gtcggagcct cggtcggaca gcgtgtagat ggttgcgccc tgggcatcgc cctcgtgacg
     1861 cggcaaggcg tcggcgtgga tcgacacgaa cagtgccgcc gactgggtgc gggctattcg
     1921 gacgcggtcg gcgagcggaa tgaaggtatc gtcggtgctg gtcatcacca cgcgatattt
     1981 gccgctcttc tcgatgcggt cgcgcaacgc cagcgcaaat ccaaggacca gcgtcttttc
     2041 gctttcgcca cccgcctgcg tgccattgtc gatgccgccg tggccgggat cgattaccac
     2101 cacaggacgc ggatcggcag gcgctctcgg cttggatgaa attaccgccg caggcagcgt
     2161 cgcggccgac gcgtcagcga tcgccggcct gagttcgggc cagccttcag acgcgtgcga
     2221 cggaacagcg gcggcgtctg tttgctccag ctccaccacc agccgcggcg gctggccgtt
     2281 agccgcatcc agcatataag agtttgcgat ccgggccggg ccggtgagat cgagcaccat
     2341 ccgcgaccct cccggcatca ccatgccgta tcggaaagcc ctgatcagcc cgcgcccggc
     2401 ggtgccgacg ccggcaggca tctgaaagct gacctggggg aagtccacta ccacacggta
     2461 tggatcggtc agcgtaaagg cgcgaaactg gacggcttgg tcgaggtcga ggacgaatcg
     2521 ggtcttcttg tcgtccccgg ctaggcggac atcggacgcg atcgggaaat tcgcggcggc
     2581 ggaggatggc ggctgacttt gggccgcccg aacggcggtg acatccgggc acacaattgc
     2641 tgcggcgcac ataagggcgc atcccagcca aacacgacta tttgtgcggc ccgccaccga
     2701 atccgtgcct ccgagcagcc tcttacccca ataggaccac agggttaatc gaaccttatc
     2761 gcacttccac cgaataaggc agggtgttgc cgcccagcga cgcttccctt gcacaggtct
     2821 ctcaatccac gtatgtaaaa ggggctgatg gctaaaactc ccggttgtgt cgctttcagc
     2881 ctcccggtgc agcgccggaa ccttcaacag aagattccag
//
LOCUS       DASWQN010000051         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1456116, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000051 DASWQN010000000
VERSION     DASWQN010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456116"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VGC82_00820"
     CDS             <1..818
                     /locus_tag="VGC82_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0421847.1"
                     /translation="AANRAAGRRRMMRHDLETLRAGSTSADMLLLIHGINPISPKAPF
                     VAALARTRNVIAPSHPGFGASPLPPDFDTMYDLVNLYLDVLDNLPDGEIAVAGFGFGG
                     WIAAELAVAGHRRLRQLILVDPVGIKIGGREDRDIVHMFNTNPIELNRRSWHDPAARP
                     AGIYGLGWQAVIDDSMTDAEMISLARNWDSLCLFAWRPHMFNPQLKHWLHRISVPTLV
                     LWGESDRIVTPDYGRHYAGLIPSAIFETIAEAGHHPELEQPEAFVASVKRFLS"
     gene            815..1183
                     /locus_tag="VGC82_00825"
     CDS             815..1183
                     /locus_tag="VGC82_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421848.1"
                     /translation="MIFIIRTEAGQVPVLDAKAVLDIKMVRRTPSIVPDSAGRIDCFC
                     LGDPAAAQAAGWPVVPVHDGWYADDTNGGMAIWGQGMFWEVRDTPFTVRQACGPEASP
                     SAPRHDEDMDLNVRQAIFRG"
     gene            complement(1215..2213)
                     /locus_tag="VGC82_00830"
     CDS             complement(1215..2213)
                     /locus_tag="VGC82_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0421849.1"
                     /translation="MTNSILRIGIAGAGHFGRYHALKVAASDRAKLMGIYDPDLERAK
                     TVGWEAGASEIGFEDMLPMIDALIVAAPAEVHHELSIRALRAGKHVLVEKPIAATLRQ
                     ANEMAALAKERGLVLQVGHLERFSGAYRAIAGRIGVPLYIEAMRIAPFKPRGTDVSVI
                     LDLMIHDLDLVLALVDSEIESVDAVGAPVASPHEDIANARLRFCNGAVATVTASRISS
                     RTERRMRIFAQDSYLAADFSARSLTVIGRAAGDPPAGQQPVPGLAGFAIEEVSWEDHD
                     SLAAEHAAFVASVLDRAPVLVDAGVGRRALAAALAVGDSMRTSRERMSASGLIRAG"
     gene            2389..>3104
                     /locus_tag="VGC82_00835"
     CDS             2389..>3104
                     /locus_tag="VGC82_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421850.1"
                     /translation="MLSLALLLFIPLSLILKYGLAADPIWLFLTGAIAIATLADWIRR
                     ATEQLAERAGSAIGGLLNVSFGSISELLLSLFVLAQGDVAVVKAQITGSIIGTSLLGL
                     GLAMLIGGFKRERQTFNRANTGLLSTLLILVVVALLLPAVFDLTARAGTRDTFPTDER
                     LSIGASAVLLLLYSANLVFTLITHRDIFAGDEPRATNGWSLAKSLGVLIGGTILVALQ
                     AELVSEALTATASQLHVSKA"
BASE COUNT          534 a         1041 c          937 g          592 t
ORIGIN      
        1 ctgccgccaa tcgagcagcg ggccgccgcc gaatgatgcg ccatgacttg gaaaccctgc
       61 gtgccggttc gacttcggcc gacatgctgc tgctgatcca cggcatcaat ccgatcagtc
      121 ccaaggcgcc attcgtcgcc gctctggcgc gcacgcgcaa cgtcatcgca ccgtcgcatc
      181 ccggtttcgg cgcctcgccg ttgccaccgg atttcgatac catgtacgac ctggttaacc
      241 tttatctgga cgtgctggac aatttgccgg acggcgaaat cgccgttgcc ggtttcggct
      301 tcggcggctg gatcgcggcg gagctggcgg ttgccggtca tcgccgcctg cggcaactga
      361 tcctggtcga tccggtcggc atcaagattg gtggccgcga agaccgcgac atcgtccaca
      421 tgttcaacac caacccgatc gagttgaacc gccgaagctg gcacgatccg gcggcgcgtc
      481 ccgcgggcat ttacggcctg ggctggcagg cggtcatcga cgacagcatg accgatgcag
      541 aaatgatttc cctggcgcgc aactgggatt cgctctgcct gtttgcctgg cgcccgcaca
      601 tgttcaaccc acaactgaag cactggctgc accgcatcag cgtcccgaca ctcgtcttgt
      661 ggggtgagag cgatcgtatc gtcactcccg attacgggcg tcactacgcc ggcctgattc
      721 ccagcgcgat cttcgaaacg attgccgagg ccggccacca tccggaactg gagcagcccg
      781 aggcttttgt cgccagcgtg aaacggttcc tgtcatgatc ttcattattc gcaccgaagc
      841 cggtcaggtg ccggtgttgg acgcgaaagc ggtgctggac atcaagatgg tccggcggac
      901 gccttccatc gtgccggaca gcgccggccg gatcgattgc ttctgcctcg gtgacccggc
      961 ggcagcacag gccgccggct ggccggtggt gccggtgcat gacggctggt acgccgacga
     1021 taccaacggt ggtatggcga tctgggggca ggggatgttc tgggaggtgc gcgatacgcc
     1081 cttcaccgtc aggcaggcct gcgggccgga ggcttcgccc tcggcgccgc ggcatgacga
     1141 agatatggat ctcaacgtgc ggcaggcgat cttccggggg tgacctaacc cgcggaaccg
     1201 ctgcggcagg cttgttagcc ggcgcggatc agacccgaag cgctcatgcg ttcccgcgat
     1261 gtccgcatgc tgtccccgac ggccagtgcc gccgccagcg cccgccgccc aacacctgcg
     1321 tccaccagga ccggcgcccg gtccagcacc gacgccacga acgccgcatg ctccgccgcc
     1381 agcgaatcat gatcctccca cgaaacttcc tcgatggcga acccggccag acccggcact
     1441 ggttgctgcc cggctggcgg gtcgcccgcg gcacgtccga tcaccgtcag gctacgcgcc
     1501 gaaaaatccg ccgccagata ggaatcctgc gcgaatatcc gcatccggcg ttcggttcgc
     1561 gatgaaatcc ggctggcggt gaccgtcgcc acggcaccgt tgcagaaccg cagccgagcg
     1621 ttggcgatgt cttcgtgtgg actggcaacc ggggcaccaa cggcatcgac gctctcgatc
     1681 tcgctgtcca caagcgccag caccaggtcc agatcgtgaa tcatcaggtc cagaatgacg
     1741 gatacgtccg ttccacgcgg cttgaacggc gcgatacgca tcgcctcgat gtacagcggc
     1801 acgccaatcc ggccggcgat ggcccgatac gcgccggaga accgctccag gtgcccgacc
     1861 tgcagcacaa gcccccgctc cttggctagc gcggccattt catttgcttg ccgcaacgtc
     1921 gccgcaatcg gcttttcgac cagcacatgc ttgccggccc gtaaggctcg gatggacagc
     1981 tcatgatgaa cctcggccgg cgcggcgacg atgagcgcat cgatcatggg cagcatgtct
     2041 tcaaagccga tttcgctcgc tccggcctcc catcccacgg tttttgcacg ctccagatcc
     2101 ggatcgtaaa tgcccatgag cttggcccgg tccgatgcgg cgaccttcag cgcgtggtaa
     2161 cggccgaaat ggccggcacc ggcaattcca atgcggagga tggaattcgt catgactgca
     2221 acctgggtac cgatgatggc cgaacctagc tgcacacaca ttctgtctga ccgtctcctg
     2281 tcaagagggt gcgatcaaac gcggcggcgg gtcggcggcg ttgttgaggc ggttcaatta
     2341 ccgcccgccc gccatcaccc gccacgttca tcaaaggtcc tggtcgagat gctttcactc
     2401 gcgcttctgc ttttcatccc gctatccctg atcctgaaat acgggctcgc cgccgatccg
     2461 atctggttgt tcctgacagg agcgatcgct atcgcgacct tggccgactg gatccggcgc
     2521 gccaccgagc aattggccga acgcgcaggc agtgccatcg gcgggctgct gaacgtcagt
     2581 ttcggcagca tttccgagct actgctgtcg ctgttcgtcc tcgcgcaggg cgatgtggct
     2641 gtcgtgaagg ctcagatcac cgggtcgatc atcggtacca gcctgcttgg cctgggcctg
     2701 gcgatgctga tcggcggctt caagcgcgaa cggcaaacgt tcaaccgggc caacaccggg
     2761 ttgctttcta cgctccttat tctggtggtc gtcgcgctgc tgctacctgc cgttttcgat
     2821 ctcaccgccc gggccggaac acgcgatact tttcccaccg atgaacggct tagcatcgga
     2881 gcttcggccg tcctgctgct gctctattcc gccaatctcg tcttcaccct gatcactcat
     2941 cgcgacatct tcgccggcga cgaaccacgc gcgaccaacg ggtggagcct ggcaaaatcg
     3001 ctgggcgtat tgatcggcgg cacgatcctt gttgcgctac aggcggaact tgtgtcggag
     3061 gcgctgaccg cgacggcctc ccagcttcat gtgtcgaagg cctt
//
LOCUS       DASWQN010000052         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2116165, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000052 DASWQN010000000
VERSION     DASWQN010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116165"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1327
                     /locus_tag="VGC82_00840"
     CDS             170..1327
                     /locus_tag="VGC82_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0421851.1"
                     /translation="MTHNGSRGTVHAAKPMRVLMVHWDGGGNMPPQRALARELKRRGH
                     DVHVLTHNTLAETVMADGGTFHALDTAGQHDPTQPRSYDEENAFVQQHVCSSPAFAAD
                     FLNTYDALSPDVCLVDAMLITTLNAAIERGLNFAAVNHLAWNPAGAAFLNSFAAVLPG
                     RAAGSTFYGLLDRVPLVLATSYPEFGTQPDVAPHIHFVGPIREPVAPAPWPRRFPDRP
                     FVLVSLSSIFQEQDATLRRICDALAPLPLEVLVTTGRSIAPEALAVSGCVEARSFVPH
                     DAVLPFVDLVVTHAGLGTSMYSAGTGVPCLCLPNGRDQDDNAARLVALGLGVILSPAA
                     TPAEIRDTVMAMLGDKVLLAACRSFAARVNRFGDLMRAADLVVECVLARPS"
     gene            complement(1241..>1603)
                     /locus_tag="VGC82_00845"
     CDS             complement(1241..>1603)
                     /locus_tag="VGC82_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY0421852.1"
                     /translation="HDVAVVLVETASRFARDLAVQLTGHAVLRARGIELVPVDAPTHF
                     TDPTPTAEMVRQILGAVSQFEKASLVAKLRHARERVKATTGRCEGSTRSRWPGEHTFH
                     DKISGAHQVAEAVHAGRE"
BASE COUNT          264 a          520 c          513 g          306 t
ORIGIN      
        1 gaatacataa gcgcagccga ttatgacggc gcgaaggcgc attaacgtct tcatatatta
       61 agttgcatat tcgacatatt tagcgtccgc gagccacaaa gtgggcgcca aatctgctgg
      121 cggatcctga ttgccgcatc atcaacagca tcacgggagg ggcgctgaca tgacacataa
      181 cgggtctcgt ggcacggtgc atgccgccaa gccgatgcgg gtgctgatgg ttcactggga
      241 tggtgggggg aacatgccgc cacagcgtgc cctggcacgc gaactgaaac gccgcggcca
      301 tgacgtccac gtgctgacgc ataacacact ggccgaaacc gtcatggcgg atggcgggac
      361 gtttcacgcg ctggacaccg cgggccagca cgacccgacc caaccgcgtt cctatgatga
      421 ggaaaacgcg tttgttcagc agcacgtttg cagctcgccg gccttcgctg cggattttct
      481 caacacctat gatgcgctta gtccggacgt ttgcctggta gatgccatgc tgatcaccac
      541 gctgaacgcg gcgatcgaac gcggactcaa ctttgccgcc gtcaatcacc tggcctggaa
      601 tcccgcaggc gcggcattct tgaactcgtt cgccgcggtc ttgccaggac gcgcggcggg
      661 ttcaaccttc tacggcctgc tcgatcgcgt tccgttggtg ctggccacca gctatccgga
      721 gttcggcacg cagccggacg ttgccccgca catccacttc gtcggcccaa tccgggagcc
      781 ggtcgcaccc gcgccgtggc cccggcgatt tccggacagg cctttcgtgc tggttagcct
      841 gagcagcatc tttcaggagc aggacgcgac gttgcggcgt atctgcgacg ccttggcgcc
      901 cctgccgctg gaggtcctgg taacgaccgg ccggagtatc gcgccggagg cgctggccgt
      961 ttcgggctgc gtggaagcgc gatcatttgt cccgcatgac gcggtgctgc cattcgtgga
     1021 tctggtcgtc acccatgcgg ggctcggaac gtcgatgtac agcgcgggga caggcgtgcc
     1081 gtgtctgtgc ctgcccaacg ggcgcgacca ggacgacaac gccgcccgtt tggtggcgct
     1141 ggggctggga gttatcctct cgcctgccgc aacgcctgcc gagatacgcg acacggtcat
     1201 ggccatgctt ggcgacaaag ttttgctggc agcctgtcga tcattcgcgg cccgcgtgaa
     1261 ccgcttcggc gacctgatgc gcgccgctga tcttgtcgtg gaatgtgtgc tcgcccggcc
     1321 atcttgatcg agtgcttccc tcgcagcggc ccgtcgtggc tttcacccgc tcgcgggcat
     1381 ggcgcagctt ggccaccagg ctggccttct cgaactggct gaccgcgccc aggatctggc
     1441 gcaccatctc ggccgtcggc gtcgggtcgg tgaagtgggt tggcgcgtcc accggcacca
     1501 gctcgatccc gcgggcccgc aagaccgcat ggccggtcag ttgcacggcc aggtcacggg
     1561 cgaagcggct ggcggtctcg accagcacca ccgccacgtc gtg
//
LOCUS       DASWQN010000053         4554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1852303, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000053 DASWQN010000000
VERSION     DASWQN010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852303"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VGC82_00850"
     CDS             <1..580
                     /locus_tag="VGC82_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0421853.1"
                     /translation="FDFRMLDPEHPENGAELARRWPIAKFPLLVDGETTVFETSAIIE
                     HLAAFHPGQVKLIPEDAKAAVPVRMLDRVFDSHVMGPMQAIVSDALRPEEARDPYGVT
                     QARTALDTIYAWLDQALVGRQWAAGDTFTLADAAAAPALFYADWAHPIGGGRSTLKAY
                     RARLLARPSFARCIDDARPYRPFFPLGAPDRD"
     gene            625..1092
                     /locus_tag="VGC82_00855"
     CDS             625..1092
                     /locus_tag="VGC82_00855"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY0421854.1"
                     /translation="MIMTSENLELSVTRLIHAPVETVWKIATERMEEWWCPKPWTVEI
                     IEQDWRAGGRSAIVMRGPNGEEMPQEGVFLEVSPGKRFVFTDAFATGWHPKAPLMVGT
                     MEFADEGGKTRYTGSARHWTKEAYDQHKTMGFEQGWTAVAEQLAALAEAETKA"
     gene            complement(1128..1991)
                     /locus_tag="VGC82_00860"
     CDS             complement(1128..1991)
                     /locus_tag="VGC82_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY0421855.1"
                     /translation="MELGRLGVWYSPDKHADVAAMGAFVRTVEKLGYDTLWYPESRGY
                     ESFSVAGYMLSQTTTLKLGSSIASIYARDAFTARRGMISLNQLYGGRFILGLGVSHVP
                     MVEGLRGHVYEKPVPAMRAYLNGITKGEAGAADFPLCIAALGPLMLKLAGQMTRGAVP
                     YNTTPRHTAEAARILGPGKWLAIEQKVLLETDPAKARALGREELARYLTLDNYRNSWL
                     GMGFTEADLSNGGSDAFIDAMVLWGSADQVKAGLRAHFAAGATHVAIQPLHAAGNTAG
                     RDEILAALVDT"
     gene            complement(2004..3191)
                     /locus_tag="VGC82_00865"
     CDS             complement(2004..3191)
                     /locus_tag="VGC82_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY0421856.1"
                     /translation="MARAFEGIRIIDITHVLAGPFAAYQLGLMGADVIKVEDPNDPDQ
                     SRDSGTDNDLNHAGMGTGFLTQGSNKRAITLNLKTEQGRDILKKLVLTADVLVENYRP
                     GAFEALGLGYEDLRKINPKLIYASFSAFGQGGPKRQRTAYDHVIQSTSGIMAMTGTPE
                     VNPIKIGAPAIDYATGTMGAYALSAALFQRERTGQGQRVDMAMLDVAMILMASHVTGY
                     LRNGKHPRPSGNRTGHATSMAYHTAEGMVMLGASNLRQQKRLWTVLERPDLIKRTNDE
                     RDVDHDREMRTLAELMLTRTAAEWEAYLQERHVPASRVRTMGEALADPQLGSRGLLHR
                     HEGAPGIPGSVTVPVAAFKFDHDGPKVDRPPPMFGQHNDEVLAELGYSQADIAGFRSA
                     KII"
     gene            complement(3537..4376)
                     /locus_tag="VGC82_00870"
                     /pseudo
     CDS             complement(3537..4376)
                     /locus_tag="VGC82_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          840 a         1502 c         1376 g          836 t
ORIGIN      
        1 gttcgacttt cgaatgctcg atccagagca tccggagaac ggcgcggaac tggcacggcg
       61 ctggccaatc gccaagttcc cattactcgt tgacggcgag acgacggtgt tcgagaccag
      121 cgcaatcatc gagcatctcg cagcatttca ccccgggcag gtgaagctga tcccggagga
      181 tgcgaaggcc gccgtgccgg tacggatgct cgatcgcgtg ttcgacagcc atgttatggg
      241 ccccatgcag gctatcgtgt cggatgcgct ccgccccgaa gaagcgcgcg atccttatgg
      301 cgtcacgcaa gcccgaaccg cactcgatac gatctacgcg tggcttgacc aagccctggt
      361 ggggcggcaa tgggcggccg gcgatacttt cacgctggcc gacgcagctg ccgcgcctgc
      421 gctgttctac gccgactggg cgcacccgat cggagggggt cgctcgacgc tcaaggccta
      481 tcgcgcgcgc ctgctcgcgc gtccctcatt tgcccgctgc atcgacgacg cccgtcccta
      541 ccggcccttc ttcccgctcg gcgcccctga ccgggactga cccctgaccg ggactgaccc
      601 tgaccggggc tgatatcaag gagaatgatc atgaccagcg aaaacctcga actctccgtc
      661 acccgcctga tccacgcacc cgtcgagacc gtctggaaga ttgccacgga acgcatggaa
      721 gaatggtggt gccccaagcc ctggacagtc gagatcattg agcaggactg gcgcgccggc
      781 gggcggtcgg cgatcgtgat gcgcgggccg aatggcgagg agatgccgca ggagggggtg
      841 ttcctggagg tgtcgcccgg caagcgcttc gtcttcaccg acgcgttcgc gaccggctgg
      901 cacccgaagg ctccgctcat ggttggtacg atggagttcg ccgacgaggg cggaaagacc
      961 cgctataccg gcagcgcacg ccactggacc aaggaagctt acgaccagca taagacgatg
     1021 ggctttgaac agggctggac cgctgttgcg gagcaactcg ccgcgctggc ggaagccgaa
     1081 acgaaggcat agatacggct acgttcagac ccacccaccg ctggcgttta cgtatccacc
     1141 agcgccgcca gtatttcgtc gcgcccggcg gtattcccag ccgcgtgcag cggctggatc
     1201 gcgacatgcg tcgccccggc ggcgaaatgc gcccgcaacc cggccttcac ctgatccgcc
     1261 gaaccccaca gcaccatcgc atcgatgaat gcatcggacc cgccgttcga aagatcagcc
     1321 tccgtgaacc ccatgccaag ccacgaattc cggtagttgt ccaacgtcag gtaccgagcc
     1381 aattcttccc gccccaatgc ccgcgccttt gccggatcgg tttcgagcaa gaccttctgc
     1441 tcgatcgcca gccacttccc cggccccagt atcctggccg cctccgccgt atgcctcggc
     1501 gtggtgttgt acggcacggc tcctcgcgtc atctgcccgg ccagcttcag catcagcgga
     1561 cccagcgcgg cgatacaaag cggaaaatcc gccgctccgg cttccccctt cgtgatcccg
     1621 ttcaggtaag cccgcatcgc cggcacgggc ttttcgtaaa catgcccgcg cagcccctcg
     1681 accatcggga catgactcac ccccaggccc agaatgaacc ggccgccata caactggttc
     1741 aggctgatca tgcctcgccg ggcggtaaac gcatcccgag catagatcga ggcgatcgaa
     1801 ctgccgagct tcagggtcgt tgtctgcgac agcatgtagc cggcgaccga gaacgactca
     1861 tagccacgcg attccggata ccaaagcgtg tcgtatccca gcttctccac cgtcctgacg
     1921 aacgcaccca tggccgccac gtcggcgtgc ttatcgggcg aataccaaac cccgagacgt
     1981 cccagttcca tgttcagatc ctgtcaaatg atcttcgcgg accgaaaacc cgcgatgtcg
     2041 gcctggctgt agccgagttc cgccaggacc tcgtcattgt gctggccaaa catcggcgga
     2101 ggccgatcca ccttcggccc gtcatgatcg aacttgaagg cggcaacggg aaccgtgaca
     2161 gatcccggga tgcccggcgc gccctcgtgc cggtgcagca ggccgcgcga accaagctgc
     2221 gggtccgcca gcgcctcgcc catcgtccgc acgcgggacg ccggcacatg gcgctcctgc
     2281 aaatacgctt cccactccgc cgccgtacgg gtcagcatca attcggccag ggtgcgcatt
     2341 tcccggtcat gatccacgtc gcgttcatcg ttggtgcgct tgatcaggtc gggccgctcc
     2401 agcaccgtcc acagccgctt ctgctgccgt aaattcgacg ccccgagcat caccatgcct
     2461 tcggcggtgt ggtaggccat cgaggtcgca tgtccggtgc ggttgcccga cggcctcgga
     2521 tgcttgccgt tgcgcagata cccggtcaca tggctcgcca tcagaatcat cgcgacatcc
     2581 agcatcgcca tatcgaccct ttggccctgt ccggtccgct cccgctggaa tagtgcggcg
     2641 gacagcgcat aggcgcccat cgtgccggtc gcgtagtcga tcgccggggc gccgatcttg
     2701 atcgggttga cctcgggcgt gccggtcatc gccatgatgc cggaggtgga ctgaatgacg
     2761 tggtcgtagg ccgtccgctg ccgcttcgga ccgccctggc cgaatgccga aaacgatgcg
     2821 tagatcagct tcggattgat cttgcgaagg tcttcgtagc ccaaaccgag tgcctcgaac
     2881 gcgccgggcc ggtaattctc caccagcaca tccgccgtca gcaccagctt cttcaggatg
     2941 tcccgcccct gctcggtttt caggttcagc gtgatggcgc gcttgttgga cccctgggtc
     3001 aggaagccgg tgcccatgcc ggcatggttc aggtcgttat cggtgccgga atcccggctt
     3061 tggtccgggt cgttcgggtc ctccaccttg attacgtcgg cccccatcag cccgagctga
     3121 tacgccgcga acgggccggc aaggacgtgg gtgatgtcga tgatgcggat accttcgaac
     3181 gctcgggcca tcggtgtccc tcctgggttc cgccgacggt gccgcctggg cagcgaggcg
     3241 tcaaccggcg ggcggtggcg tggtggctaa ggccgggcgt acggtacgct gccggggaca
     3301 tcgccataac ggcgcgtgtc tgccgttgtc cggaatggat acaagtggga aggatcaagc
     3361 cgaccggagc tggccgaaag cggaatggca ggttccggct tacatacggc acaaagcaga
     3421 cattggcgca atgatggcgg ccggcagctg acggcccagg caagtcactg tagagcttcg
     3481 cggatgattt aaaagcgtac attcacgagc gaccttgctg agagcctgac tcgaaatcac
     3541 gctgctgtct catagtccct ggcgatcctg cgtaccagca ggaaagccag ggacagcatg
     3601 aaccaagcct gtgacgaaga gatcaacgcc tcgaagtcct ttgccaggcg gcgggctcgg
     3661 tttacccagc cgaatgtccg ctcgacgatc cagcgcttcg gcagcacgat aaaccccttg
     3721 accgcgcgat cggtgcgttt gaccacgctc agcctgatcc ggctcgcctc gtatgcggcg
     3781 cgggcggttt cgtcaccagc atatccgcca tcgacaaacg cgagtttaac ccaaggactt
     3841 ttctggatca cggcttccag caagtccggc aacgcgtcgc gatcctgaac gtccgccgtt
     3901 gtcacctgac acttgatcgg caggccgaga gtatcgaccg cgatgtggcg tttgcggccc
     3961 ttgacctttt tgcccgcgtc gtagcccttg gggccacccg cctcggtggt cttgacggac
     4021 tggctgtcga cgatgacgac gctcggcgcc gcgtcccgtc cttccgcctc acgaacagcc
     4081 atgaccagga cgctaacgat ctattcccac agaccgctgt cgcgccaggc atagaagcgg
     4141 ttctgcacgg tggtgaaggg cggaaaatcc tttggcaggt agcgccacgg gcagcctgcg
     4201 cggaccaggt aaaacaaggc attgaggatg gcgcgtggat cggttggctt gcgtccggac
     4261 ggtttgggca cgggcagcaa tggctgcacc aatgcgaact cctcatccga caggtcgctt
     4321 gcgtagcgct ggcggatcac cgcatatttc tctcgatcgg tctcgttcca aggcatggcg
     4381 aatcgctcgt cttgttgcag agcaagcgaa tcgcacccaa cgagaccggt caacctctca
     4441 atgtttggac ctgagcgatt tgattccgat accgtttccg gtcaggctct gattaagatg
     4501 cggacggagg agcacaattg cactgaaaca ggctctgagc ggcctaacgt cttg
//
LOCUS       DASWQN010000054         6182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_928676, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000054 DASWQN010000000
VERSION     DASWQN010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928676"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..366
                     /locus_tag="VGC82_00875"
     CDS             106..366
                     /locus_tag="VGC82_00875"
                     /inference="COORDINATES: protein motif:HMM:NF019324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEY0421857.1"
                     /translation="MGAQLNIKSDNAYHLASRLSELTGESLTTVVTKALQTELDREQR
                     LRDKAALRARLLALADEISADMPEDVTSNHDWLYDEETGLPR"
     gene            363..758
                     /locus_tag="VGC82_00880"
     CDS             363..758
                     /locus_tag="VGC82_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0421858.1"
                     /translation="MILDSSAILAIVLREPDRFRLVDAMLETVPRRMSVANWLEATMV
                     VDRRGGALSISRFEDFLRNAEIELIPVSVSQATLARRAWRTFGRGVHPARLNYGDCFA
                     YALAKETREPLLFKGNDFAQTDIEPALKD"
     gene            761..2812
                     /gene="glyS"
                     /locus_tag="VGC82_00885"
     CDS             761..2812
                     /gene="glyS"
                     /locus_tag="VGC82_00885"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY0421859.1"
                     /translation="MPELFLELFSEEIPARMQRGGAAELERAVTGALAAIAPANIATW
                     FGPRRIALRAEIAAEVAAAASIERGPRASAPEQALTGFLRKHNASREQLRQEGDYWVL
                     EKNAEAVSAAALIAGVMPGLLRRFPWPKSMRWGGSSSFVWVRPLRRIVCLLDGDVVPF
                     DLRDGADDGHGLASANLTEGHRFHAPGAFPVVSAANWQESLTARQVLVDAAHRKRVIA
                     DRIAGLAAEKSLTVVDDPGLLEEVAGLVEWPVPHLGRIADEHMDLPPEVMQVSMRVNQ
                     RYFALRTTGGSAAPWFAFVANIQADDNGAAIIAGNERVLRARFSDARHFWDLDRKTRL
                     ETRIPALDKIVFQARLGTQGDRVRRLSGLAGTIAPLVGANSARAARAAELAKADLVSG
                     MVGEFPELQGVMGRYYALHDGEPPEVADAVRDHYAPRGPAEAAPSAPVSIAVALADKL
                     DQLTGFFAIGEKPTGSGDPYALRRAALGVIRLIRENGLRVHLRQLLAATGAGQATVHA
                     VFEFVMERLVVQLRADGARYDVLNAVFGAKGPGREQDDDLVALLGRTDSITGLLQGPD
                     GANLLTAYRRASNILRIEERKDGPFDDAPDPGLYREAAENDLDRALAASPVVLRLLSE
                     ENYAGAMDTMARLRAPLDLFFEKVTVNAPEPDLRRNRLRLLSQVRSIMDQIADFSRIE
                     G"
     gene            2825..5494
                     /gene="ppdK"
                     /locus_tag="VGC82_00890"
     CDS             2825..5494
                     /gene="ppdK"
                     /locus_tag="VGC82_00890"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEY0421860.1"
                     /translation="MTKWVYSFGAGHKEGRADMRNLLGGKGANLAEMATIGLPVPPGF
                     TITADVCTAFYDNNRTYPEDLKDQVDAALAVIEKAVGLNFGDKAHPLLVSVRSGARVS
                     MPGMMDTVLNLGLNDITVEGLATAANDPRFAWDSYRRFIQMYGSVVLGVDHHRFEEII
                     EHAKLEANVIEDTALTAQHWRDVVEGYKDLVHQETGKPFPQDPQEQLWGAIGAVFGSW
                     MNPRAVTYRRLHDIPAAWGTAVSVQAMVFGNMGNDCATGVCFTRDPSTGENMFYGEYL
                     VNAQGEDVVAGIRTPQPLSSSVAKPGEIPLEQAMPNAYAELMQVRSTLEKHYKDMQDI
                     EFTVQQNKLYMLQTRNGKRTAAASLRMAVEMAREGLIDEAEAVMRVNPSSLDQLLHPM
                     LDPNAARTLFGKGLPASPGAASGMVVFSADEAESRAAKGEAVILVRIETSPEDIHGMH
                     AAKGILTTRGGMTSHAAVVARGMGRPCVAGAGGISVDYNSQVLAAGGKSIRAGEIITL
                     DGATGEVFVGPVAMIEPAMSGDFGTLMQWADKSRRMGVRANAETPMDAETARKFGAEG
                     IGLCRTEHMFFNPERILAVRQMIISNAESGRRTALARLLPFQREDFRQLFTIMAGLPV
                     TIRLLDPPLHEFLPHAEQEMQEVADSLGTDINTIRQRAEELKEANPMLGHRGCRLGIA
                     YPEIYEMQARAIFEGAIAVAKETGKAPHPEIMIPLVGMKKELEITRAQVEKIAAEVFA
                     ESGTTIEYSVGTMIELPRAAILADKIAEVADFFSFGTNDLTQTVFGLSRDDAGKFLPQ
                     YVEAGILPKDPFVSIDVEGVGEMVRIAAEKGRQTKKQLKMGICGEHGGDPASIAFCEQ
                     VGLDYVSCSPFRVPVARLAAAQAALGTASDRTA"
     gene            complement(5909..>6182)
                     /locus_tag="VGC82_00895"
     CDS             complement(5909..>6182)
                     /locus_tag="VGC82_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421861.1"
                     /translation="GSHGVVIAAWAGGLACEVEFDEPRHLLVTIKAANLTVTMPNATK
                     PELIRISSFICSIRRVVILYPARLRLFRAQELIDALKRYLSEPSAR"
BASE COUNT         1144 a         1869 c         2028 g         1141 t
ORIGIN      
        1 acgctgtccg cagcaaatgt gccgcactcg ccggaaggtg catgacattc agcagccgat
       61 atcctattct accggttagg tgacatattc taccaaggtg aggctatggg tgctcaattg
      121 aacatcaaaa gtgacaacgc gtaccatctg gcgtctcgct tgtccgaatt gacgggtgaa
      181 agcctgacga cggtggtcac gaaagcgctt cagacggaac tcgatcgcga gcagcggctg
      241 cgcgacaagg cggcgcttcg agcgcgactg ctggcgctgg cggatgaaat cagtgcggat
      301 atgcccgagg acgttacctc taaccatgat tggctgtacg atgaggagac gggcttgcct
      361 cggtgattct cgactcctcg gctatccttg ccatcgtttt gcgggagccg gatcggtttc
      421 gcctcgtcga tgcgatgctg gaaaccgtgc cgcgccggat gtctgtcgcg aattggctgg
      481 aggccaccat ggttgtcgat cgcaggggtg gcgcgttgtc gataagccgg tttgaggatt
      541 ttctacgaaa cgccgaaatt gaactgatac cggtgtcggt gtcgcaggcg acgctcgcgc
      601 gacgcgcctg gcgtaccttc gggcgcggcg tccatccggc tcggttgaac tacggcgatt
      661 gcttcgccta cgcgcttgcc aaggaaaccc gcgaaccgtt gctgttcaag ggcaacgact
      721 tcgcccagac cgacatcgag ccggcactga aagactgaac atgcccgaac tttttctgga
      781 actgttcagc gaggaaattc ctgcccgcat gcagcgcggc ggggcggcgg agcttgagcg
      841 tgccgtgacg ggtgcgcttg ccgccattgc cccggccaac atcgccactt ggttcggccc
      901 ccgccgcatc gcacttcggg ccgaaatcgc cgccgaggtt gccgccgccg cgtccatcga
      961 acgcgggccg cgcgccagtg cgccggagca ggcgttgacc ggcttccttc gcaagcacaa
     1021 cgccagccgc gaacagcttc gccaggaagg cgactactgg gtgctggaga aaaacgctga
     1081 ggctgtatcg gccgccgcat tgatcgcggg tgtcatgccg ggcctgctgc gccgcttccc
     1141 gtggccgaaa tccatgcgct ggggcggctc cagcagcttc gtctgggtcc ggcccttgcg
     1201 ccggatcgtc tgcctgctcg atggtgatgt cgtcccgttc gacttgcgcg atggcgccga
     1261 cgacggtcac gggctggcta gtgccaattt aacggagggc catcgctttc acgcccctgg
     1321 cgcgttcccc gtcgtcagcg cggccaattg gcaggagagc ctgaccgccc gccaggttct
     1381 ggtggacgcc gcccatcgca aacgcgtcat cgccgaccgc attgccggtc tggcggcgga
     1441 gaagtcgctg acggtggtgg acgatcccgg cctgctggag gaggttgccg gcctggtcga
     1501 gtggccggtc ccgcatctgg ggcgcatcgc ggatgagcac atggacctgc cgccggaggt
     1561 catgcaggtg tcgatgcggg tgaaccagcg ctacttcgcg ttgcggacca ccggtggttc
     1621 ggcggcgcct tggttcgcct tcgttgccaa catccaggcc gacgacaatg gtgcggcgat
     1681 catcgccggg aacgaacgcg tactgcgcgc ccggttttcg gacgcaaggc atttctggga
     1741 cctcgatcgc aagacacggc tggaaacccg tattcccgca ctggataaga tcgtttttca
     1801 ggccagactg ggcacacagg gcgaccgggt gcgccggttg tcggggctgg cgggaacgat
     1861 cgcgccgctg gtgggggcca attctgcgag ggccgcacgc gccgccgaac tggccaaggc
     1921 tgacctggtc tccgggatgg tgggggagtt tccggagctg caaggtgtga tgggccgcta
     1981 ctatgctctc cacgacgggg aaccgcctga agtcgccgac gcggtccgcg accactacgc
     2041 cccgcgcggt ccggcggagg ccgctccgtc cgccccggtt tccatcgccg tcgcactggc
     2101 ggacaaactg gatcaactca caggtttttt tgcaattggc gagaagccga ccggctctgg
     2161 tgacccgtac gcgctgcgcc gcgcggcgtt gggcgtgatt cggctgatcc gcgagaacgg
     2221 cttgcgggtg catctgcgtc aactgctggc cgccaccggc gctggccagg cgacggtcca
     2281 cgcggtgttt gagtttgtca tggaacgcct ggtcgtccag ttgcgggccg atggcgcccg
     2341 ctatgacgta ttgaacgcgg tgttcggtgc caagggcccg ggtcgcgagc aggacgacga
     2401 ccttgttgcc ctgctgggtc ggaccgattc catcactggt ctgctgcaag gtccggacgg
     2461 cgcaaatttg ctgaccgcct accgccgagc ctccaatatc ctgcggatcg aggagcgcaa
     2521 ggatggtccg ttcgacgatg cgcctgatcc cggcctgtac cgggaggctg cggaaaacga
     2581 cctcgatcgg gcgcttgccg cttccccggt ggtattgcgt ctgctgagcg aggaaaatta
     2641 tgccggcgcc atggacacca tggctcgctt gcgtgcaccg ctggatttgt tcttcgaaaa
     2701 agtaaccgtg aacgcccccg aaccggattt gcgccgcaat cgtttgcgtt tgctcagcca
     2761 ggtccgatct atcatggatc agatcgccga cttttcccgt atcgaaggct aatgaggtcc
     2821 atacatgacc aagtgggttt acagcttcgg cgccggccac aaagaaggcc gagcggatat
     2881 gcgcaacctg ctggggggaa agggggcaaa cctggcggag atggcgacca tcggcctgcc
     2941 cgtcccgccg ggcttcacca tcaccgccga cgtatgcact gcattctatg acaacaaccg
     3001 cacctatccg gaggacctga aggatcaggt cgatgccgcg ctggccgtga tcgagaaagc
     3061 cgtcggcctg aacttcggcg acaaggcgca tccgttgctg gtgtcggtcc gctccggcgc
     3121 gcgcgtttcg atgccgggca tgatggacac cgtgctgaac ctcggcctga acgacatcac
     3181 cgtcgagggc ttggccacgg cagccaacga tccgcggttc gcctgggatt cctaccgccg
     3241 cttcatccag atgtatggat cggtggtgct tggggtggat catcaccggt tcgaggaaat
     3301 catcgagcac gccaagctcg aagccaacgt gatcgaggac actgccttga ccgcgcagca
     3361 ttggcgggac gtggtagaag gctacaagga cctggtacac caggaaaccg gtaagccgtt
     3421 cccgcaggat ccgcaggagc aattgtgggg tgccatcggc gcggtgttcg gatcatggat
     3481 gaacccgcgt gcggtcacct atcggcgcct gcatgacatc ccggcagcct ggggcaccgc
     3541 cgtgagcgtc caggccatgg tgttcggcaa catgggcaac gactgcgcga ccggcgtttg
     3601 ctttacccgc gacccctcca ccggggagaa catgttctat ggcgagtacc tggtgaatgc
     3661 gcagggtgag gacgtggtcg ccggcatccg cacgccgcag ccgctgtcgt cgtccgtggc
     3721 caagccaggt gagatcccgc tcgaacaagc catgcccaac gcctatgcgg aactgatgca
     3781 agtccgttcg accttggaaa aacactacaa ggacatgcag gacatcgagt ttaccgtgca
     3841 gcagaacaag ctgtacatgc tgcagacacg caacggcaag cgaactgccg cggcctcgct
     3901 gcgcatggcg gtggagatgg ctcgcgaagg cctgatcgac gaggccgagg cggtgatgcg
     3961 ggtcaaccca tcctcgctgg accagctttt gcacccgatg ctggacccga atgccgcgcg
     4021 aacgctgttc ggcaaggggc tgcctgccag cccgggcgcg gcctccggca tggttgtgtt
     4081 ctccgccgat gaagccgaat cgcgtgccgc caagggtgag gcggtgatcc tggtccggat
     4141 cgagacaagc cccgaagaca tccacggcat gcacgccgcc aagggcatcc tgaccacccg
     4201 tggcggcatg accagccatg ctgccgtggt ggcgcgcggc atgggccggc cttgcgtcgc
     4261 cggtgccggc ggcatcagcg tcgattacaa cagccaggtg ctcgcggccg gcggcaagtc
     4321 tatccgagcc ggtgagatca tcacactgga tggcgctacg ggtgaggtct ttgtcggccc
     4381 cgtcgccatg atcgagccgg cgatgtccgg cgatttcggc acgctgatgc agtgggccga
     4441 caagtcccgc cggatgggcg tccgggccaa cgcggaaacc ccgatggacg cggaaaccgc
     4501 gcgcaaattt ggcgccgagg ggatcggcct gtgccgcacc gaacacatgt tcttcaatcc
     4561 cgagcgtatt ctggcggttc gccagatgat catctccaac gccgagtccg ggcggcgcac
     4621 ggcgctggca cgcctgctgc cgttccagcg ggaggacttt cggcaactgt tcaccatcat
     4681 ggcggggctc ccggtgacga tccggctgct cgatccgccg ctgcacgaat tcctgccgca
     4741 tgcggagcag gaaatgcagg aggtcgcgga cagcctgggc accgatatca acaccatccg
     4801 ccagcgggcc gaggagctga aggaagccaa tcccatgctc ggtcatcgcg gttgccggct
     4861 gggaatcgcg tacccggaga tctatgaaat gcaggcgcgc gcgatcttcg agggtgccat
     4921 cgccgttgcc aaggaaaccg ggaaggcgcc gcatccggaa atcatgattc cgctggttgg
     4981 catgaaaaag gaactggaga tcacccgggc acaagtggaa aagatcgccg cggaagtatt
     5041 cgccgagagc ggtacgacga tcgagtacag cgtcgggacg atgatcgaac tccctcgggc
     5101 cgcgatactg gcggacaaga tcgcagaggt cgccgatttt ttcagcttcg gcacgaacga
     5161 ccttactcag acggtctttg ggctgtctcg tgacgacgcg ggcaaatttc tgccgcagta
     5221 tgtcgaggcg gggatcctgc cgaaggaccc gttcgtttcg atcgacgtcg agggcgtagg
     5281 cgagatggtc cgcatcgcgg cggaaaaagg tcggcagaca aaaaagcagt tgaagatggg
     5341 aatctgtggg gagcatgggg gcgatccggc ctcgatcgcg ttttgcgagc aggttgggtt
     5401 ggactacgta tcgtgcagtc cgttccgggt gccggtcgcg cgtctggcgg cagcacaggc
     5461 ggctttgggt acggcgtccg accggacggc ttgaaaggtc ggtactagat tccagacctt
     5521 atgatcatcg agacttgcat ctggacgacc gcggttcgag gcagactcgc tgattacaac
     5581 cttgctccgg gatgcgacct gttacggcag gctcgggaac ataaggtcgg tacgagttgg
     5641 cgtagccgct gaagaggaac ggatcggtca tcaccccgtc gatcatcggc tgataaagtc
     5701 cgccgcgctg ccgtcgcggc catcttcgct tccgcagccg agtgatacgt gccacggatc
     5761 acacatgata ctgtcgttca gggacaagag gacggcagcg gttgtccgtg gagaacttcc
     5821 gagaacttgt ccgccccacc tgctgacggc gaagcggcgg aagctgggtt gtctggacgc
     5881 agcagccact cttgaggatc tgcgatcact acctagcgct gggttcggag aggtagcgct
     5941 ttagcgcatc aatcagttct tgcgcccgga acaatcgaag ccgtgcagga taaagaatta
     6001 caactcttcg gattgaacag ataaacgaac ttatgcggat caactcgggc ttagtggcgt
     6061 tcggcatcgt cacggtcagg ttcgcggcct tgatcgtgac cagcaggtgg cgaggttcgt
     6121 cgaactccac ttcacatgcc agtccaccag cccaagccgc gatgaccacg ccatgtgatc
     6181 cg
//
LOCUS       DASWQN010000055         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2512296, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000055 DASWQN010000000
VERSION     DASWQN010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2512296"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VGC82_00900"
     CDS             complement(<1..577)
                     /locus_tag="VGC82_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemopexin repeat-containing protein"
                     /protein_id="HEY0421862.1"
                     /translation="MLTQLTGAVALPDDRCILFSDTLYWTLDLVSGSLADGPLAIADD
                     FAGVWPSGKVVPVYWGCGQVFFFSNGQFTRYDLAARKVQYDAPLPVAGAWPGMWPEGP
                     DAAFNGLNGKVYFFRGGEYLRYDITLDRTDPGYPRNIADMWSGVWADGVDAALCPDGM
                     QVLFFRGTDYLVFNLAADAVTAGPLPVSGIAL"
     gene            882..1691
                     /locus_tag="VGC82_00905"
     CDS             882..1691
                     /locus_tag="VGC82_00905"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0421863.1"
                     /translation="MAASQENQDPSGAIPALDYDAIVIGAGMSGLYQLYRLRELGMRV
                     RVLEAGTGVGGTWYWNRYPGARFDSESYSYSYSFSPELLEEWNWSEHFAGQPETLRYL
                     NHVADKFDLRRDIQFRSRVTAAHYQEATRSWDVTLEDGTTFRSRFLITAIGPLSAPTM
                     PRIEGIDTFQGQSFHTARWPHEQVSFAGKRVAVIGTGATGVQTIQEVAKTVGHLTVFQ
                     RTPNWCAPLHNSKIDPERWRRSKPITRGCSNAVRKRSPASSIPRIPAARSR"
     gene            1589..2521
                     /locus_tag="VGC82_00910"
     CDS             1589..2521
                     /locus_tag="VGC82_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421864.1"
                     /translation="MAKIKADYPRMFERCQETFACFLHTPDPRGTFEVTPEEREAFFE
                     KRYSEPGFGIWQGNFRDILTDPKANDAITDFVARKIRQRVRDPKVAEKLIPTNHGFGT
                     RRVPLETKYYEVYNQDNVRLVDIKETPIERITPTGIKTSDADYEFDMIIYATGFDAIT
                     GSFDRIDIRGRDGLRLKDKWAQGPKTYLGVQVEGFPNMMMLMGPHTALGNIPRSIEYN
                     VDWVTRLIAAMRQRNLTRVEATPEGVASWTDHVKALGVGLLSNEVNSWMTGVNTNVEG
                     KQTRIIARYSGSAPAYRARCDEVAANGYRELALA"
BASE COUNT          558 a          881 c          840 g          423 t
ORIGIN      
        1 ccagggcgat cccgctgacc ggcagcggcc cggcagtaac ggcatcggct gcgaggttaa
       61 aaacgaggta gtccgtgccc cggaagaaca gcacctgcat cccatcggga caaagcgccg
      121 catcgacgcc gtcggcccaa acgccgctcc acatgtcggc gatgttgcgc ggatagcccg
      181 ggtcggtgcg gtcgagcgtg atgtcatagc gaagatattc gccgccgcga aagaaataga
      241 ccttgccgtt gagcccgttg aacgcggcgt cgggtccttc cggccacatc ccgggccagg
      301 cgccggcgac aggcagcggc gcgtcgtact gcaccttgcg cgccgccagg tcgtagcgcg
      361 tgaactgccc gttcgagaag aagaagacct gaccgcagcc ccagtaaacg ggaacaacct
      421 tgccgcttgg ccaaacgccg gcgaagtcat cggcgatcgc cagcgggcca tcggccaggc
      481 tcccggacac caggtcgagc gtccagtaaa gcgtgtcgct gaacaatatg caccggtcgt
      541 ccggcagcgc caccgcgccg gtcagttgcg tcaacattgg ctactctccg atgaggtgtg
      601 tccgcctgac ctgtacgcgt cggtgccgct gcgcgtccgt gcgcccgatc acagtgccta
      661 cggaatcgag gtcagcttgc gcaaaacctg gtcctggcgc ggcggattcg actccggcca
      721 accaacgggc gttcggaccg aagcaccgtc ttgttgttga acgcccacat cccggataaa
      781 cctctgcgtt aaatggacgg aaggcctcgg cgcgacgggc gcggcgccga tcgaatgaaa
      841 ccgacttgga ctttcgaccc cgcatgcagg agcagcgaca aatggccgcc tcgcaggaaa
      901 accaggaccc atccggcgcg ataccagctc tggactacga cgccattgtt atcggtgcgg
      961 ggatgtcggg cctctaccag ctctaccgct tgcgggaact tgggatgcgc gtgcgggtgc
     1021 tggaagccgg caccggcgtc ggcggcacct ggtactggaa ccgttatccc ggcgcgcgct
     1081 tcgactccga aagctactcc tacagctact ccttctcgcc ggaactgctg gaggaatgga
     1141 actggtccga acatttcgcc ggccagccgg agacgctgcg ctacctcaac cacgtcgccg
     1201 acaagttcga cctgcgccgc gatatccagt tccgcagccg tgtgaccgcg gcgcactacc
     1261 aggaggcgac gcgaagctgg gatgtgacgc tggaagacgg cacaacgttc cgctcgcgct
     1321 tcctgatcac cgccatcggg ccgctgtcgg cgccgacgat gccgcggatc gaggggatcg
     1381 ataccttcca gggccaatcc ttccacaccg cgaggtggcc acacgagcaa gtcagcttcg
     1441 cgggcaagcg cgtggccgtc atcggcaccg gtgccaccgg cgtccagacg atccaggagg
     1501 tcgccaagac cgtcgggcat ctgacggtgt tccaacgcac gccgaactgg tgcgcgccct
     1561 tgcacaacag caagatcgat ccggaacgat ggcgaagatc aaagccgatt acccgcggat
     1621 gttcgaacgc tgtcaggaaa cgttcgcctg cttcctccat accccggatc cccgcggcac
     1681 gttcgaggtg acacccgagg agcgtgaggc gttcttcgag aagcgctaca gtgaaccggg
     1741 cttcggcatc tggcagggca attttcgcga tatcctcacc gaccccaagg ccaacgacgc
     1801 catcaccgat ttcgtcgctc ggaaaatccg ccagcgagtg cgtgacccga aggtcgccga
     1861 gaagctgatc ccgaccaacc acggcttcgg cacccgccgc gtgccgctgg agacgaagta
     1921 ttacgaggtg tacaaccagg acaacgtaag gctggtggac atcaaggaga cgccgatcga
     1981 gcggattacc ccgaccggca tcaagaccag cgatgccgac tacgaattcg acatgattat
     2041 ctatgccacg ggtttcgacg ccatcaccgg cagcttcgat cgcatcgaca tcaggggccg
     2101 cgacggactg cggctaaagg acaaatgggc gcaggggccg aaaacctacc tcggcgtcca
     2161 ggtcgaggga tttcccaaca tgatgatgct gatgggccca cacaccgcgc tgggcaacat
     2221 cccgcgcagc atcgaataca acgtcgattg ggtgacgcgc ctgatcgccg ccatgaggca
     2281 gcggaacctg acccgcgtcg aggcgacgcc agagggcgtg gcgagctgga ccgaccacgt
     2341 caaagcgctc ggggtggggc tgctgtccaa cgaggtcaac tcctggatga ccggcgtcaa
     2401 caccaatgtg gagggcaagc agacccgcat catcgcgcgc tacagcggca gtgcgcccgc
     2461 ctaccgcgcg cgatgcgacg aggtggcggc aaacggatat cgggaactcg cgctggccta
     2521 aatggaaaca gccgaagtcg atgctgcgcc tagtctaggc aaacgccttg atgacgagcg
     2581 tggccacgcc gccgatcagg acagccagct gccacatcac gagcgtcaag cgaccgccga
     2641 tttctcaatc ttcaggtcga tgttggcgaa gcgcatctcg tagggcgtga ttgcctctgc
     2701 cg
//
LOCUS       DASWQN010000056         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1984348, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000056 DASWQN010000000
VERSION     DASWQN010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984348"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VGC82_00915"
     CDS             <1..699
                     /locus_tag="VGC82_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0421865.1"
                     /translation="ASQFGLLAEQIMPAPTAVGMAAWRVTSSGELLRDIAVSTWRAFA
                     GFAIGGSIGFSLGMLNGLSGLSEKLTDSTLQMVRNIPHLALIPLVILWFGIGEEAKLF
                     LVALGVFFPIYVNTLHGVRSVDPQLMEMGRAYGMSERAIFWRVVFPGAMPSIFVGLRY
                     GLGIMWLTLIVAETISASSGIGFMAMNAREFMMVDVVVVSILIYAALGKLADSVARLL
                     ERTCLSWNPVYAEA"
     gene            703..1509
                     /locus_tag="VGC82_00920"
     CDS             703..1509
                     /locus_tag="VGC82_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0421866.1"
                     /translation="MNISVLDHASDTRIQFSSAGSAMHLRGVSKAFGEHQVLHGIDLE
                     IPAGQFVAIVGRSGCGKSTLLRLLAGLDTPTGGRIELDGRTAGWRDSVRLMFQEPRLL
                     PWQRVAANVEVGLSHASAGDGRRRQAIEAIAQVGLSGRDTEWPSVLSGGQKQRVALAR
                     ALVSHPRILAFDEPLGALDALTRIEMQALIEQVWLEKGFTAVVVTHDVTEAVALADRI
                     LLLEAGRVAMDVAVDLPRPRRRGDPAAAAIEGQILNRLLGDRSAATVMAS"
     gene            2200..>3739
                     /locus_tag="VGC82_00925"
     CDS             2200..>3739
                     /locus_tag="VGC82_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY0421867.1"
                     /translation="MRPAGMTGWLTALGLAVTFGMLVLGGVILFDLRAGAWRQAEKAS
                     DNLILMLSQDIARNITLYDLSIQGVIKAQAEPALAKADPIIRYMALFDTAATADYLGS
                     LILANAHGDVLANSESLDFEPYNIAFREHFSVHEQRSDIGLFISRPFDGRMVRDKIIV
                     LSRRISNPDGSFAGVASGGMRIAYFRDLFDKLKLGTNGAISLLRSDGRLIMRRPFHAE
                     DIDKDLYYASTFQTYLTAPHGHFVGTAVLDGMERLYTFHRIDNLPLYLSIAVPTDDIL
                     AEWWPKAMSIGVILIALCVTSVVLCLLFRREIQRRFVAENSLREAAGNLLVMAATDGL
                     TGIPNRRSHEDALAQEWRRAIRNETSLGVLMIDVDCFKLFNDHYGHQMGDEVLKAVAS
                     CFSTTLRRPGDIAARYGGEEFVALLPETELAGAMNVAEAVRAAVADLAVAHVRSPSGY
                     VTVSVGVAIARPVPGDPSAALVREADDALYQAKRSGRNRVANNGRGIDPGLDLENLSA
                     VGCYQ"
BASE COUNT          664 a         1154 c         1176 g          745 t
ORIGIN      
        1 gcatcccagt tcggcctgct cgccgagcag atcatgccgg cgccgacggc cgtgggcatg
       61 gctgcgtggc gagtgacaag cagcggcgaa ttgctccgcg acatcgccgt cagcacctgg
      121 cgggccttcg ccggctttgc catcggcggc agcatcggtt tctcgctggg catgctcaac
      181 ggcctgtccg gactgagcga gaagctgacc gacagcaccc tgcaaatggt ccgcaacatc
      241 ccgcatctgg cactgattcc cctggtcatc ctttggttcg gcatcggcga ggaagccaag
      301 ctgttccttg tggccctcgg cgtcttcttc ccgatctatg tcaacacgct gcacggggtg
      361 cgttcggtcg atccgcaatt gatggaaatg ggccgggcat acggcatgtc ggaacgggcg
      421 atcttctggc gcgtggtgtt tcccggtgcg atgccgtcga tcttcgtcgg tctgcgctac
      481 ggcctgggca tcatgtggct gacgctgatc gtggcggaaa cgatctccgc atcctccggc
      541 atcggcttca tggcgatgaa cgcccgggag ttcatgatgg tcgatgtcgt cgtcgtgtcc
      601 atcctgatct atgccgcgct gggaaaactc gccgacagcg tcgcacgctt gctggaacgc
      661 acatgcctgt cctggaaccc tgtttacgcg gaagcctgag ccatgaatat cagcgtcctc
      721 gaccatgcgt ccgatacaag gatccagttc agctccgctg gatcggcgat gcacctgcgc
      781 ggcgtgagca aagcgttcgg tgagcaccag gttctgcacg gaatcgacct cgagatcccg
      841 gctggccaat tcgtcgccat cgtcggccgc agcggttgcg gcaagagtac gctgctgcgc
      901 ctgctggccg ggttggacac acccaccggc ggacggatcg agctcgatgg ccggaccgcc
      961 ggctggcggg acagcgtccg cctgatgttc caggagcccc gattgttacc ttggcagcgc
     1021 gtggcggcaa acgtggaggt tggattatcc catgcttccg ccggcgatgg ccgccgccga
     1081 caggcaatcg aggcgattgc ccaggttggc ctgtccgggc gcgacacgga atggccttcg
     1141 gttttgtcag gcggccagaa acagcgcgtt gccctggcac gcgcgctggt gagccatcct
     1201 cggatcctgg ctttcgacga accgctcggc gcactggacg ccctgaccag gatcgagatg
     1261 caggcgctga tcgagcaagt ctggctggag aagggtttca ccgccgtcgt ggtgacccac
     1321 gacgtgacgg aggcggtcgc cctcgccgat cgcatcctcc tgcttgaggc aggccgggtg
     1381 gcgatggacg tggcggtgga tctgccccgt ccccggcgac gaggagatcc ggcggcggcg
     1441 gcgatcgaag ggcaaatctt gaaccggttg ctgggcgata gatcggctgc gaccgtgatg
     1501 gcgtcctaaa cacctcgtgt agagcgcaat cggccgaggt taatccagcc ttcgacggaa
     1561 atgctctttc agggtctgat gaaaccgctt cgaccagctg ttcctgcccg gcatcgccgt
     1621 gcatgtggac tgatatagtc gcgatcacgc ggcctttgcc cccgatcggt cgcacacagc
     1681 atcaggtcgc gctggcgcga ggttctccgg aaactcttgc ttcgcgtcgg cttacaccgg
     1741 tgctggggca gcctttgtgc tggaagggcg atcgcgctcc gcagggcgcc cagggtgatc
     1801 acgccgggcg gacgtccgac gcgtttgaat catgcgatca gacaaaggtg gagcattttc
     1861 ggcctgtacc gtcaggctga aatgctctag cagcgcgcgg cacgccccgc cgcgatcccg
     1921 atggacgccg ctatctaccg gccggcgcgg gaaattgcga tagagtcaca accgccttaa
     1981 ccctttgttc acggatacgc tttaggacgt tgcttgaacg atatgtgtat cggcatcaag
     2041 tgcggatgaa cgaacggctt acgcgacaac cgatcgccag agcgatcgac ccccatcggc
     2101 attgcccgga aacgtccaac atagtcccat cgatcggcgc atgttttgaa catatctcat
     2161 accacccgaa acatggttca aatcgtacga ctcggtgcca tgcggccggc cggcatgacc
     2221 ggctggctga ctgcactcgg cttggcggtc acgttcggga tgctggtcct gggcggcgtg
     2281 atcctgttcg atctgcgggc tggcgcgtgg cggcaggccg aaaaggcatc cgacaacctt
     2341 attctcatgc tcagccagga catcgcccgc aacatcacgt tgtacgactt gtcgatccaa
     2401 ggcgtgataa aggcccaggc cgaaccggcg cttgccaagg ccgatccgat aatccgttac
     2461 atggctttgt tcgacacggc ggcgaccgcg gattacctgg gttcgctgat tctggcgaat
     2521 gcgcatggcg acgtcctcgc aaactccgaa tcgctggatt tcgaacctta caacattgca
     2581 ttccgggagc atttttcagt ccacgaacag cgatcggata taggcctttt catcagccgt
     2641 cccttcgacg gccgcatggt gcgcgataag atcatcgtgc tgagtcgtcg catcagcaat
     2701 ccggacggca gcttcgccgg cgtcgcctcg ggcggcatgc ggatcgccta ttttcgcgat
     2761 cttttcgaca agctcaaact tgggaccaac ggcgccattt cgctgctgcg cagcgacgga
     2821 cggcttatta tgcgtcgccc attccacgcc gaggatatcg acaaggatct gtactatgct
     2881 tccacgtttc agacatatct gaccgcaccg cacgggcatt tcgtcggtac cgccgtgctc
     2941 gacgggatgg agcgcctata cacgttccat cgcatcgaca atctgccgct ttacctaagc
     3001 atcgccgtcc cgaccgacga catcctcgcg gagtggtggc ccaaggcgat gagcatcggc
     3061 gtgatcctga tcgcgctgtg cgtaacctcg gtggttttgt gcctgttgtt ccggcgagag
     3121 attcaacggc ggttcgtggc cgagaattcg ctgcgggaag cagccggaaa cctgttggtg
     3181 atggccgcca ccgacggcct gaccggcatt ccgaaccgca ggagccacga ggacgcctta
     3241 gctcaggaat ggcgccgcgc gatccgaaac gagacgtcgc tcggcgtcct gatgatcgac
     3301 gtggactgct tcaaactgtt caacgatcat tacggtcacc agatgggcga tgaagtcctc
     3361 aaggccgtcg cctcgtgctt ttcgacgacc ttgcgccgtc cgggcgacat cgcagcgcgc
     3421 tatggcggtg aggaattcgt cgcactgttg cccgaaacgg agctggcggg tgcgatgaac
     3481 gtcgcggaag ctgtccgggc cgcggttgcc gatcttgccg ttgcccatgt ccggagtccg
     3541 agcggctatg tgaccgtaag cgtcggcgtg gccattgcgc ggccggtgcc cggagatcca
     3601 tcggccgcgc tggtaaggga ggccgatgac gcgctttacc aggccaagcg ttccggccgg
     3661 aaccgcgtcg ccaacaatgg ccgaggcatc gatccggggt tggacctcga aaacctctcc
     3721 gcggtgggct gctatcagg
//
LOCUS       DASWQN010000057         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1984574, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000057 DASWQN010000000
VERSION     DASWQN010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984574"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VGC82_00930"
     CDS             <1..542
                     /locus_tag="VGC82_00930"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0421868.1"
                     /translation="KGVTPQEVAYDMLIADEGKGFIFTCLVNYANYDLEAVKTMYSRQ
                     HVIPGLSDGGAHVAFISDASFPTFLFSYWGRDVPDGRMELHDLVRRQTREPAHFAGLH
                     DRGVLAPGMKADINVIDFPNLALERPNMVVDLPAGGRRLMQKARGYAATVKAGQVTYR
                     NGEATGALPGGLVRGQCVG"
     gene            complement(725..1417)
                     /locus_tag="VGC82_00935"
     CDS             complement(725..1417)
                     /locus_tag="VGC82_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0421869.1"
                     /translation="MRGSLGEKIGEGATADIHAWAPGQVLKLFKPGVSQQHSWWEARM
                     TRAVFAAGGLAPEVLDEVTLEGRFGIVLPYLDGPTLLQLLRARAMTPEQVGAILATVY
                     ISVHKTPPPPELLSLRDWFAAVSRQSGDMLSKHIATGILALIERLPPGDGLCHGDLHP
                     SNVIMTADGPRIIDWIGSVRAGAALDLARCHVTFCDLVYAPEGADPELPRALNAAVQS
                     EYARLAGMSPRR"
     gene            complement(1813..>3018)
                     /locus_tag="VGC82_00940"
     CDS             complement(1813..>3018)
                     /locus_tag="VGC82_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY0421870.1"
                     /translation="LIPMAFTITLTMLPRSKQAVGLAMFAVSATFAPAIGPTIGGYLT
                     ETYGWQYIFYVNLIPGAVMLATLWPTLRPAPMNLSLLRRGDWFGIATLAIGLASLQTV
                     LEEGNKDDWFGSSFIVRLSVIAAVSLTLFVWIELTSRNPLLNLRLLMRRNFGLGSLAT
                     VILGMALYGSVYLLPVYLSQMQGYNAEQVGMVLAWTGLPQLVLIPFVPLLMRHVDARV
                     LVGIGFGLFALSCFLNLGITSDYAGDQFFVPNLVRAVGQALVMTPLSALATGGIEREN
                     AGSASAMFNMMRNLGGSVGIAVLQTFVSKREQFHSSIVTARVSLLDEATRQRIASLQA
                     HFLAGEASDPATAWHDAVVQIGRTIRAQSFLLAYSDAFYLMGAALLLALATSLAMRKS
                     SGGGGGGAH"
BASE COUNT          579 a          964 c         1007 g          468 t
ORIGIN      
        1 ggaaaggtgt cacgccgcag gaagtggcgt acgatatgct gatcgccgat gaaggaaagg
       61 gcttcatctt cacctgcctg gtcaactacg cgaactatga cctggaggcg gtgaaaacga
      121 tgtattcccg ccagcatgtc attcccggcc tgagcgacgg cggcgcgcat gtcgcgttca
      181 tctccgacgc gtctttcccg accttcctgt tcagctactg gggccgcgac gttccggacg
      241 gtcgcatgga gctgcatgat ctggtgcggc gtcagacgcg cgagccggcg cacttcgccg
      301 ggttgcacga ccgcggcgtg ctcgccccgg ggatgaaggc cgacatcaac gtgatcgact
      361 tcccgaacct ggcgctggag cgtccgaaca tggtcgtgga tctgccggcc ggcgggcggc
      421 ggctgatgca gaaggctcgg ggttatgcgg cgacggtcaa ggcgggtcag gtcacctacc
      481 gcaacggcga ggcgacgggt gccttgccgg gcgggctggt gcgagggcag tgcgtgggct
      541 gaccgtaggt ctgggcaatc catcggccag cgtgcgggcg atggcctcgg gctggccgat
      601 cagtcctctg aacgcagggc ggcttccacg cgctggatca gccgctcccg ccgggcaggg
      661 ttggtcgccc tttcggcaag ggcgaaggcg cggaggatgg gcaaataggg ctttatcgcc
      721 gccgtcagcg ccgcggggac atgccggcca gccgcgcata ctcggactgc acggccgcat
      781 tgagcgcgcg cggcagctcc ggatcggcgc cttccggggc gtagacgaga tcgcaaaagg
      841 tgacgtggca gcgcgcaagg tcgagggcgg cgcccgcgcg gaccgaaccg atccaatcga
      901 tgattctcgg accatccgcc gtcatgatca cgttgctggg gtgaaggtcg ccatggcaca
      961 gcccgtcccc cggcggcagg cgctcgatca aggcaaggat gccggtggcg atgtgcttcg
     1021 aaagcatatc gccggactgc cgcgacacgg cggcgaacca gtcgcgaagg gagagtaact
     1081 ccggcggcgg gggcgtcttg tgaacggata tatagacggt cgcgaggatt gcgcccacct
     1141 gctcgggcgt catggcgcgg gctcgcaaga gctgcagcag ggttggtccg tcgaggtacg
     1201 gcagcacgat gccgaagcgg ccctccaggg tgacctcgtc cagcacttcc ggcgccaggc
     1261 cgccggcggc gaagacggcg cgggtcattc gcgcctccca ccaactgtgc tgctgcgaga
     1321 cgccgggctt gaacagcttc aggacctgac cgggcgccca ggcatgaatg tccgccgtag
     1381 cgccttcgcc gatcttctcc cccagggatc cccgcatcgt ttgtcctccc ccacagggag
     1441 caaacaccga cggcgggcaa agcaaaaccc ccttctcaaa gccaaaggcc gctcctggca
     1501 gctgggtcac ggccgggagc cgatctaacc cacaggccac gaatgtcgcg tttgggccac
     1561 aaggtcggct gtctcgtgaa gggtctcgcc gaccggtaga taatcccgaa ccttaagacg
     1621 cggccggcga tcctttgccg actccggcta aaacatcaga gcggtttcgc ggtgactgga
     1681 aacaggggtt cttttgtggc tgcagagaaa gtcttgagtg gcgtgccaag cacggccatg
     1741 acatgccgac gccaagcggc gaactggccg gcacctttcc agtcaacgtg aaaacgctcc
     1801 aatgggcgcc ggctaatggg cgccgccgcc gccgccgccg ctcgacttgc gcatcgccag
     1861 ggatgttgcc agcgccagca gcagggccgc gcccatcagg tagaaggcat cgctgtaggc
     1921 cagcaggaag gattgcgcgc gaatggtgcg cccgatctgc accacggcat cgtgccaggc
     1981 ggtggcgggg tcgcttgctt cgccggccag gaaatgcgcc tgcaaggagg cgatgcgctg
     2041 acgggtcgcc tcgtccagca aggatacgcg tgccgtgacg atgctggagt ggaactgctc
     2101 gcgcttggac acgaaggtct gcagcacggc gatgccgacg gagccgccca ggttgcgcat
     2161 catgttgaac atcgccgatg ccgaaccggc gttttcccgc tcgataccgc cggtcgccag
     2221 cgccgacagc ggcgtcatca ccaaagcctg ccctacggcg cgcaccaggt tcggcacaaa
     2281 gaactggtcc ccggcgtaat cggacgtgat cccaaggttc aggaagcagc tcagcgcaaa
     2341 cagaccaaag ccgatcccaa ccagcacacg agcatcgacg tggcgcatca gcagcgggac
     2401 gaacggaatc agcaccagtt gcggcaatcc cgtccaggcc aggaccatcc ccacctgttc
     2461 ggcattgtag ccctgcattt gcgacagata gaccggcagc agatacacgg acccatacaa
     2521 cgccattccg agaatgacgg tggccaggct gccaaggccg aaattccgcc gcatcagcag
     2581 gcgcaggttc agcagcgggt tgcggctcgt cagctcgatc cagacgaaca gcgtcagcga
     2641 gacggcggcg atcaccgaca accgcacgat gaacgaagaa ccgaaccagt cgtccttgtt
     2701 gccttcctcg agcaccgtct gcaatgacgc gaggccgatc gccagggtgg cgatgccgaa
     2761 ccagtcgccc cgccgcagca gcgacagatt catcggcgcc ggccgcaggg tcggccagag
     2821 tgtcgccagc atgaccgcgc cgggaatcag gttgacgtag aaaatatatt gccagccata
     2881 ggtctcggtc aggtagccgc cgatggtcgg tccgatcgcc ggcgcgaacg tggccgacac
     2941 cgcgaacatc gcgagcccga ctgcttgctt cgaccgaggc agcatcgtca atgtgatggt
     3001 gaatgccatc gggatcag
//
LOCUS       DASWQN010000058         5109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2512687, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000058 DASWQN010000000
VERSION     DASWQN010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2512687"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /gene="hemH"
                     /locus_tag="VGC82_00945"
     CDS             complement(<1..818)
                     /gene="hemH"
                     /locus_tag="VGC82_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY0421871.1"
                     /translation="MQGNKLGLERGDHGKGVAPDGGISAPVVPVVVVPERDEIPETDA
                     SVPVRRKVAIVLFNLGGPDRPEAIKPFLLNLFRDPAILRVPFFVRPFLARIIARERVA
                     PATANYEILGGKSPLLGLTQEQGSALEAALPEVEAKCFIAMRYWHPFSLEAARAVKAW
                     GPDEVVLLPLYPQFSTTTTGSSLAAWRDAAVRVGLTADTTRVCCYPVDPAYATATTAL
                     VKLAYRNARAALDPVVPLRVLFSAHGLPEVIIQRGDPYQWQVEQTVAAVIGAWG"
     gene            complement(808..1839)
                     /gene="hemE"
                     /locus_tag="VGC82_00950"
     CDS             complement(808..1839)
                     /gene="hemE"
                     /locus_tag="VGC82_00950"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY0421872.1"
                     /translation="MKRLLRTLRGEAVWPPPVWLMRQAGRYLPEYRAVRAGVKDFVAL
                     CTTPALAAEVTLQPIRRYGFDAAILFSDILMLPWALGHGLEFKEGEGPVLPKLRDEAA
                     VAALDPSRVAARVAPIMETVRLVRSGLAAEGFADTTLIGFAGAPFTVACYMVEGGGSK
                     DFAAVRAMAYAQPALFQRLMNVLTEASIEYLSAQVVAGAEVLMLFDSWAGVLSPSLFR
                     MHCVAPTARIVSALKARHPGVPVIGFPRLAGVMTRDYAATGVDGVGVDTGSDIAAVAA
                     MLPAQMAVQGNLDPLAVVAGGSAMASEAAAILAAMRGRPFIFNLGHGIVPQTPPEHVA
                     ALLELVRAG"
     gene            complement(1867..3504)
                     /locus_tag="VGC82_00955"
     CDS             complement(1867..3504)
                     /locus_tag="VGC82_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0421873.1"
                     /translation="MASPELLSAATQRLSAGAWLEAEVAARAALADDPDDPQAALLLG
                     LAIAAMGEHARAAPILISAAAMRPDGDHPCVEMARLMPPLPRSVVARQFAACMRLTRA
                     DDRLRLDFAAFLLDTDQPARAEAILADGPDTAACHHLKGLARAELERFSAAADSFQRA
                     VALDPDAAASWTNLGMMLKVEGRFAESVAAHDRAVALEPNNHRFRVNRAIALLKMGNW
                     QPAWRDYEARLPMAGGPPIDALRLMPSLRPGDSLVGLTVLALHEEGFGDTLQFLRYLP
                     LLAERGARVVVCVPAALVRILRMVPGVSDVVSDIRHVPAHDFVCPIFSLPRVFGTTIA
                     TIPPVPVLAMDESLTQRWAARLPSKDLRVGLVWAGQARPSAPGFGTLDRRRSAGLAAF
                     APLADIPGLTFVSLQAGPAARQKRPPYLVLANPMQDVEDFADTAAIIASLDVVVSVDT
                     SVVHLAGLVGKPVFLMDRYDGCWRWLSDRIDSPWYPGLTIFRQEQPGDWSGAMTRAAG
                     SLLAMALFRGFGSASRGTKEAGVLDLTREPIAWRQAV"
     gene            3838..4884
                     /locus_tag="VGC82_00960"
     CDS             3838..4884
                     /locus_tag="VGC82_00960"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0421874.1"
                     /translation="MHSEPSPPQQAGAAPDRRTLLASLGGFQSPCTRRSIAQLAVTAA
                     PYAALVAFMYYAYYHISPWLSLALALPAAGFVVRLFIIQHDCGHGAYFRSRWANETVG
                     WLCSLPTFTPYHFWRRHHANHHAVWNNLDKRPTGGDIFSGCLTLAEYDALSPLRQRLY
                     RAALNPLVSQILLPPVVFLLVNRIPFDTPKKGWKKERTSVYLTNLAIAVVLLVMTLLL
                     GWRPVLLVQLPAIAVASIIGAWLFSVQHRFEHSVWERQSEWTAAGAALHGSSWLRLPK
                     VLQWFTGNIGFHHVHHLMPRVPNYRLQAAHHAQPAFAANVTGLTLWQALRAPSFTLWD
                     EARGAMIRFPRRRR"
BASE COUNT          946 a         1819 c         1508 g          836 t
ORIGIN      
        1 tcgccccacg ccccgatcac cgcggcgacc gtctgctcca cctgccattg atacgggtcg
       61 ccacgctgga tgatcacctc gggcaagccg tgcgccgaga acagcacccg cagcggcacc
      121 acagggtcca atgccgcccg agcattccga tacgccagct ttaccagggc cgtggtcgcc
      181 gtggcatagg caggatcgac agggtagcag cacactcttg tcgtatccgc cgtcaatccg
      241 acccgcaccg cggcatcgcg ccacgccgcc agcgagcttc ccgtcgttgt tgtggaaaac
      301 tgaggataca gcggcagcag caccacctca tccggccccc acgccttcac ggcgcgtgcc
      361 gcttccaggc tgaacggatg ccaataacgc atggcgatga agcacttcgc ctccacctcg
      421 ggcagcgccg cctccaacgc cgaaccctgc tcctgcgtca gtcccagcag cggcgacttg
      481 cccccaagga tttcgtaatt cgccgtcgcc ggcgccaccc gctcccgcgc gatgatcctg
      541 gcaagaaagg gccgcacgaa gaacggcacc cgcagaatgg ccggatcccg gaacaggttc
      601 agcagaaacg gcttgatcgc ctcgggccgg tctggcccgc ccagattgaa caggacgatc
      661 gcgaccttac gccgaaccgg caccgatgca tccgtttccg gaatttcgtc cctctcgggc
      721 accacgacga caggaactac cggcgcggag atcccgccat ccggcgcaac gcccttgccg
      781 tgatcgcccc gttcaagccc gagcttgtta ccctgcacgc accagctcca gcagcgccgc
      841 cacatgctcg ggcggcgttt gaggcacgat cccgtggccc aggttaaaga tgaacggccg
      901 cccgcgcatc gccgcgagga tcgccgcggc ctcggacgcc atcgccgatc cgccggccac
      961 cacggccaaa ggatccaaat tcccctgcac cgccatttgt gccggcaaca tagcggcgac
     1021 cgctgcgata tcgctgccgg tgtccacgcc gacgccatcg accccggtcg ccgcataatc
     1081 ccgggtcatc accccggcca gcctgggaaa cccaatcacc ggcaccccgg gatgccgtgc
     1141 cttcagcgcc gatacgatcc gcgccgtcgg tgccacgcaa tgcattcgga acaacgacgg
     1201 cgacaacacg ccagcccagg aatcgaacag catcagcacc tcggccccgg caacaacctg
     1261 agccgacagg tactcgatgg aagcctcggt cagcacattc atcagtcgct ggaacaatgc
     1321 cggctgtgcg taagccatcg cgcgcacggc agcgaagtct ttcgatccac caccctcgac
     1381 catgtagcag gccacggtga acggggcgcc ggcgaagccg atcagcgtgg tgtcggcgaa
     1441 gccttcggcc gccaatccgg acctgaccag ccgcaccgtc tccatgatcg gtgccacccg
     1501 ggcggcgacg cgtgatgggt ccagcgccgc cacggcggcc tcatcgcgca gcttcggcag
     1561 aaccggcccc tcgccctcct tgaactccag cccgtggccg agcgcccagg gcagcatcag
     1621 gatgtcgctg aacaggatcg ccgcatcgaa accatagcgg cggatcggct gcaacgtgac
     1681 ctcggccgcc agcgccggcg ttgtgcaaag ggcgacgaaa tccttcactc cagcccgcac
     1741 ggcgcgatat tccggcagat agcgccctgc ctgacgcatc agccaaactg gtggcggcca
     1801 caccgcctcc ccgcgcaggg tccgcagcaa ccgtttcatg ttgacgtcca ttcaccccag
     1861 ataggcctaa accgcttgtc tccaagctat cggctccctg gtcaagtcga gaacgccagc
     1921 ttctttcgtc ccacgcgacg ccgaaccgaa gccacggaac agtgccattg ccaataatga
     1981 tccggcagcc cgggtcatgg cgccggacca atcccccggc tgctcctggc ggaagatcgt
     2041 caggccggga taccacgggc tgtcgatgcg atcactcagc catcgccagc agccatcgta
     2101 gcggtccatc aggaagaccg gcttgccgac aagccctgcc agatgcacga cagaggtatc
     2161 gacgctgacc acgacatcca ggctggcgat gatcgctgcg gtgtcggcga aatcctcgac
     2221 gtcttgcatc ggattggcca gcaccaggta aggcggccgt ttctgcctcg ccgccggccc
     2281 cgcctgcagg ctgacgaatg tcaatcctgg aatgtccgcc aatggtgcga aggccgccag
     2341 tcccgcgctg cggcggcgat ccagggtgcc gaaacccggc gccgagggcc ttgcctggcc
     2401 agcccaaacc agtccgaccc gcaaatcttt gctcggcagc cgcgccgccc agcgctgcgt
     2461 cagcgactcg tccatagcca ggaccggcac cggtggaatc gtcgcgatcg tcgtgccaaa
     2521 aacccttggc aggctgaaaa tcgggcaaac gaagtcgtgc gccggtacgt gccggatgtc
     2581 gctcaccacg tccgagaccc cgggcaccat ccgcaagatc cgcactaaag ccgccggcac
     2641 gcagaccacg accctcgcgc cccgctccgc cagcagcggc aggtagcgca gaaactgcaa
     2701 cgtgtcgccg aagccctcct catgcaatgc caggacggtc aggcccacga gactatcgcc
     2761 tggacgcagc gagggcatca gccgcagggc atcgatcggc ggtcccccgg ccatcggcaa
     2821 ccgagcttcg taatcccgcc acgcgggctg ccagttgccc attttcaaca aggcaatggc
     2881 gcggttgacg cggaaccggt ggttgtttgg ctccagcgcg accgcacgat cgtgtgcggc
     2941 gacggattcg gcgaaccgtc cttcgacctt cagcatcatg cccaggttcg tccaactcgc
     3001 cgccgcatcc ggatccagcg caacggcgcg ctggaagctg tcggccgccg cggaaaaccg
     3061 ctcgagctcc gcccgcgcca aacctttcag gtgatgacag gcggcggtgt ctggtccatc
     3121 cgccaggatc gcttccgccc gcgccggctg gtccgtatcg agcaggaacg ccgcgaaatc
     3181 cagccgcagc ctgtcgtcgg cccgggtcaa tcgcatgcag gcggcaaatt gccttgccac
     3241 caccgatcga ggcagcggcg gcatcaagcg cgccatttca acgcaggggt gatcgccgtc
     3301 tggccgcatt gccgcggcgc tgatcaggat cggggcggcg cgggcatgct cgcccatcgc
     3361 ggcgatcgcc aggcccaaca gcaacgcggc ctgcggatca tccggatcat cggcgagcgc
     3421 ggcccgcgcg gcgacttcgg cttcgagcca agcgccagcc gacaaccgct gggtcgccgc
     3481 tgacaagagt tcaggtgacg ccatgggtcg gcctctgtca aaaaaggcca aggtgacagc
     3541 caaaggttaa cgcgaagtta ccggtccggc cggttttcgt ttcttaaaat taacccttaa
     3601 tcgatctgtc ggcggcgagg atcggtattg cgccaggcgg ccgcgcgacc atctctgaga
     3661 cggcttgaga tcaagcgcgc cacatatttg gtttgacaat cgataccttg tgtgtgaaac
     3721 aatgagaaat cgattggtcg tgttcccgca acatcttttc tgctacacaa ttgcgtgatc
     3781 accctgcagc tgtccaactc cggacgccgc caaccccggc cgacgaaaag accctttatg
     3841 cattccgaac catcaccgcc gcagcaagcc ggagcggcgc ctgaccggcg gacgctgctg
     3901 gccagccttg gcggctttca gtctccgtgc acccgtcgca gtattgccca gctcgcggtg
     3961 accgctgccc cgtacgccgc gctggtcgcg ttcatgtatt acgcctatta tcatatatcg
     4021 ccgtggctga gcttggccct cgccctgccg gcggcgggct tcgtggtccg gttgttcatc
     4081 atccagcacg attgcggcca cggtgcgtat ttccgctcgc gctgggcgaa cgagaccgtg
     4141 ggctggctct gcagcctgcc gaccttcacg ccgtaccatt tctggcgccg gcaccatgcc
     4201 aaccaccatg cggtatggaa caatctcgac aagcgcccca ccgggggcga catcttctcc
     4261 gggtgcctga cgctcgcgga atacgatgcg ctgtcgccgc tgcgtcagcg cctctaccgc
     4321 gctgccctca accccctggt ttcccagata ttgttgccac cggtggtgtt cctcctggtc
     4381 aaccgaattc cgttcgacac gccaaagaag ggttggaaga aggagcggac cagcgtttac
     4441 ctgaccaacc tcgccatcgc cgtagtcctt cttgtcatga ctttgctgct cggctggcgg
     4501 ccggttctgc tggtccaact gccggccatc gccgttgcgt ccattatcgg tgcctggctg
     4561 ttctcggtgc agcaccggtt cgagcactcg gtctgggagc gccagtcgga gtggacggcc
     4621 gccggcgccg cgctgcacgg cagttcctgg ctcaggctgc ccaaagtcct gcaatggttc
     4681 accggcaata tcggcttcca ccacgtccat catctgatgc cgcgggtgcc gaactaccgg
     4741 ttgcaggcgg cgcaccacgc tcagcccgcg ttcgccgcga acgttactgg attgacgctg
     4801 tggcaggccc tgcgcgcgcc cagttttacg ctttgggatg aagctcgtgg cgcgatgatc
     4861 cggtttccgc gccggcgccg gtagcagccg ccggcggcac ctgaagcgcc ggcggggtgt
     4921 agccgcgaac ccaaagaccc gcgtaggaca cacaagctcg ggtaggacat tcggcccggg
     4981 taggacattc aggcccgggt aggacacgca ggccgggtag gacacgcagg ccgggtagga
     5041 cacacaagcc tgtaccacgt tggtccgtga tacgatatga tcccatcgct cgatatgatc
     5101 ccatcgctc
//
LOCUS       DASWQN010000059         5030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2380683, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000059 DASWQN010000000
VERSION     DASWQN010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2380683"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1265
                     /gene="metZ"
                     /locus_tag="VGC82_00965"
     CDS             72..1265
                     /gene="metZ"
                     /locus_tag="VGC82_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEY0421875.1"
                     /translation="MTFDPSSYRTATRLVHGGVQRTQHGETAEALFLTSGYVYDDSEQ
                     AEATFAGTIDHYQYSRFANPTLTMLERRLCLIEDAEDCRTTATGMAAVNAALLSHLKA
                     GDRVVASRALFGSCHWIVSTLLPKFGIAADFVDGSDLAQWQAALSRPANLVLVESPSN
                     PMLEMVDLPAVAELAHAAGAIVVVDNVFATPLLQQPLKLGADVVVYSCTKHMDGQGRV
                     LGGAVLGTKKWINEVLQPFIRNTGPALSPFNAWVLLKGIETLALRVHAAGRSAAAIAD
                     FLAEQPAVTRVWYPMRADHPQQALALKQMTGGGTVVTFEVAGGKDGAFRVMNGFRLIA
                     VSNNLGDSKSLVTHPATTTHMRIGEQERARLGITDGVIRVSVGLEDVDDIRDDLRQAL
                     ERLRT"
     gene            1293..2501
                     /locus_tag="VGC82_00970"
     CDS             1293..2501
                     /locus_tag="VGC82_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0421876.1"
                     /translation="MTYAGLTRRSALAGAGIVGCGGARAQDAPPPLVIGVLTDQSGLG
                     RSVSGPPLVQAVRMAVRDTGPLPDGRSLSVITDSFELKPDDALAIARRWFDQGVSAIV
                     DVPGSAAALAVQALARSRRRSSLITGSVNPDLTGPACSSFGSSWNVDIATVAAALVRA
                     VAHAGTTSWFLVVPDSVLGLAMEAAAIQAIEENGGQLLGRSRHPAAATDFASTVTQAK
                     ASGAGAVGLCDIAPGLANQLGQFRDGGLSDQNRRVAAFLPAITDIHAAGANVADGLLL
                     ANSFYWNQNDQARSFSSRFIAAAGQMPDAAHAAAYVAVRHYLRAVVVTGSLDNDLINQ
                     EMRRAPVYFFGRSARLRLDGRLAADLSLLRVKPPDAMHGPWDHYEQIGVVPASEGYRP
                     LHRTGCALGT"
     gene            2576..3064
                     /gene="apaG"
                     /locus_tag="VGC82_00975"
     CDS             2576..3064
                     /gene="apaG"
                     /locus_tag="VGC82_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY0421877.1"
                     /translation="MSSEDSKPGRIKRPASPVVQRPGRSGGNARADYSRTTRDVIVSV
                     RSFFLADQSQPDENHFVWAYRVKIENQGPEPVQLLRRTWQITDARGRTQHVHGAGVVG
                     EQPLLEPGESFEYTSGTPLDTPSGFMVGAYHMLVPSSGESFDVAIPAFSLDSPHQSGR
                     VH"
     gene            3154..3813
                     /gene="folE"
                     /locus_tag="VGC82_00980"
     CDS             3154..3813
                     /gene="folE"
                     /locus_tag="VGC82_00980"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY0421878.1"
                     /translation="MNIVTPASPRTSGRDSETSIATVDRPTREQAEAAVRTLLQWAGD
                     DPDREGLRDTPSRVARSYLEFFIGYGIDPIALLERTFEETDGYDEIVLLRDIRLESTC
                     EHHMAPIIGRVHVAYLPHHRVVGISKLARVVDAYAKRLQIQEKLTAQIANTIQDVLEP
                     RGVAVVIEAAHQCMTTRGVHKPGVTMVTSRMLGAFRDNAATRRELLAMIGNRGSTIMG
                     E"
     gene            complement(3905..4492)
                     /locus_tag="VGC82_00985"
     CDS             complement(3905..4492)
                     /locus_tag="VGC82_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421879.1"
                     /translation="MQTNNPTRRLMLLLPLAVAACGGEPEPVFDPLRYNYLPPIQLNV
                     AAIEIQQRFIPAGVAPDVSNQDPVPPIEALRAMANDRLQAFGTANKGIFTILDASLMR
                     EADIVTGSMAVSLAILDDAGAQLGTAEARVQSRHSGQIRNIRPVLYDLTKGMMADMNV
                     EFEYQIRRNLKNWLTEGAAPDTPVEQAPLDQPTMR"
     gene            complement(4692..>5030)
                     /locus_tag="VGC82_00990"
     CDS             complement(4692..>5030)
                     /locus_tag="VGC82_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEY0421880.1"
                     /translation="EAAQEESWHTATTLAATLTERELLDDNLPSQRLLYRLFHTEGVA
                     SERARALSFGCRCSRARLASILEGFPADDLDHMAIDDDIVMTCEFCNYDFRFPRQAVQ
                     GQMPSDPKPG"
BASE COUNT          840 a         1561 c         1702 g          927 t
ORIGIN      
        1 gaggccagcg ttgagttcaa cggactctcg ctgctcattg gccgggcctc gcgtgcaagg
       61 gccctgacat catgacgttc gatccatcca gctaccgcac cgcaacgcgc ctcgttcatg
      121 gcggcgtcca gcgaacccaa cacggcgaaa cggcggaagc gctgttcctg acctccggct
      181 acgtttatga cgactccgaa caggccgagg cgactttcgc cggaacgatc gaccattatc
      241 agtattcccg cttcgccaat ccgacgctga ccatgctcga gcggcggctt tgcttgatcg
      301 aggacgccga agactgccgc acgacggcga ccgggatggc cgcggtcaat gccgcgctgc
      361 tgtcgcattt gaaggcgggc gaccgggtcg tggcgtcacg ggcgttgttc ggatcctgcc
      421 attggatcgt ttccacgctg ttgccgaaat ttggcatcgc ggcggatttc gttgatggct
      481 cggacctggc ccagtggcag gctgcgctat cgcgtcccgc caacctggtg ctggtggaat
      541 cgccctccaa tccgatgctc gagatggtcg atctacccgc ggtcgccgaa ctggcgcacg
      601 ccgccggcgc catcgtggtg gtggacaacg tcttcgcgac cccgctgttg cagcaaccgc
      661 tcaagctggg tgccgacgtc gtggtctatt cctgcaccaa gcacatggac ggacagggca
      721 gggtgctcgg tggcgctgtc ctcggcacca agaaatggat caacgaggtc ctgcagccgt
      781 tcatccgcaa caccgggccg gcgctgtcgc cgttcaacgc ctgggtcttg ctgaaaggaa
      841 tcgagacgct ggcgctgcgc gtccacgccg ccggccggtc cgccgccgcg atcgcggatt
      901 tcctggccga acagccagcg gtgacgcggg tctggtatcc gatgcgcgca gatcatccgc
      961 agcaggcttt ggcgctgaag cagatgaccg gcggcggcac cgtcgtgacg ttcgaggtcg
     1021 ccggcggcaa ggatggcgca ttccgggtga tgaatggctt ccggctgatc gcggtgtcga
     1081 acaacctggg cgactccaag tcgctcgtga cgcatccggc gacaaccacg catatgcgca
     1141 tcggcgagca ggagcgcgcc cgcctgggca ttaccgacgg cgtcatccgc gtttcggtcg
     1201 gcctcgagga tgtggacgac atcagggacg atctgcggca ggcgctggag cggttgcgga
     1261 cctaaattcg gctgccggag atcgggctcc cggtgacgta tgccggactg acgcgccgat
     1321 cagcgctcgc cggtgcggga atcgtgggct gcggaggcgc gcgggcgcaa gatgcaccgc
     1381 cgccgctggt gatcggggtg ctgacggacc aaagcggact cggccgctcg gtttcaggac
     1441 cgccgctggt tcaggcggtg cggatggcag tccgggatac cggcccatta ccagatggcc
     1501 gctcgttgtc ggtgatcacg gacagcttcg agctcaagcc ggacgatgcg ctggcgatcg
     1561 ccaggcgttg gttcgatcaa ggggtgtccg cgatcgtcga tgttccggga tcggcagccg
     1621 cgctcgcggt gcaggcgctg gcccggtcgc gtcgccggtc gagcctgata accggcagcg
     1681 tcaatcctga cctgaccggg ccggcgtgct cctcgttcgg atccagttgg aatgtggaca
     1741 tcgccacggt ggctgccgcg ctggtgcgcg cggtggcgca cgccggcacc acgagctggt
     1801 ttctggtggt gcctgattcg gtcctgggcc tggcgatgga ggccgcggcg attcaggcga
     1861 tagaggagaa tggcgggcaa ctcctgggca ggtctcgcca tccggcggca gcgaccgatt
     1921 tcgcctccac cgtgacgcag gcaaaggcct cgggcgcggg cgccgtgggc ctgtgcgaca
     1981 tcgcaccggg gctggcgaac cagcttggac agttccgcga cggcgggttg tccgaccaaa
     2041 accgcagggt cgcggcgttc ttgccggcga tcaccgatat tcatgcggct ggcgcgaacg
     2101 tggcggacgg gctgctgctc gcgaattcat tttactggaa tcaaaacgat caggccaggt
     2161 cgttctccag caggttcatt gcggctgcgg gccagatgcc ggacgccgcg catgccgcgg
     2221 cttatgtggc ggtcaggcat tatctgcgcg ccgtggtggt cacgggcagc ttggataacg
     2281 acctgatcaa ccaggaaatg cgccgtgcac cggtttattt cttcggccgt tcggctcggt
     2341 tgcgcctgga cgggcgcttg gcggccgact tgtcgctgct gcgggtcaag ccaccggatg
     2401 cgatgcacgg gccatgggat cattacgagc aaatcggtgt cgttccggct tccgagggtt
     2461 accggccgtt gcaccggact ggctgcgcct tggggacgtg acaccgctgt cctggacaag
     2521 tcgggtggat tcgtccttga ttcactgcct cgacaaggca agaaggtatg gtgtcatgtc
     2581 gtctgaagat tccaagcccg gccggatcaa gcgtccggcg tcgcccgtgg tccagcggcc
     2641 tggccgctcc gggggaaacg ctcgcgcgga ctattcgcgg accacccgcg atgtcattgt
     2701 ttcggttcgg tcgttcttcc tcgcggatca gtcgcagcct gatgagaacc attttgtctg
     2761 ggcgtaccgg gtaaagatcg agaaccaggg gcccgaaccg gtccaactgc tgcgccgcac
     2821 ctggcagatc accgatgcgc gcgggcgcac gcagcatgtg catggggccg gcgtggtggg
     2881 agagcagccg ttgctcgaac ccggggaaag ctttgaatac acgtccggca ccccgttgga
     2941 cacgccgtcc ggcttcatgg tcggcgctta tcacatgctt gtcccgtcct ccggggaaag
     3001 ctttgatgtc gccattcccg ccttcagtct ggacagcccc caccaaagcg gtagggtcca
     3061 ctagctgaca tgagatagct tgcgggcttc gaatagaccc ccatgtcggc cggggcagtt
     3121 aagcccatgg aacaagcggg aggattattc gcggtgaaca tcgttacgcc tgcctcgcct
     3181 cgaaccagcg gccgggactc ggaaaccagc atcgccacgg tggaccgtcc cacgcgggag
     3241 caggccgagg ccgccgtacg taccttgctg caatgggccg gggacgatcc ggaccgggaa
     3301 gggctgcgcg acacgccatc ccgggtcgca cggtcgtatc tggagttctt catcggctac
     3361 ggcatcgatc cgatcgcgct gctggaacgg accttcgagg agaccgacgg ctacgacgag
     3421 atcgtcctgc tgcgcgacat ccgcctggag agcacctgcg agcaccacat ggcgccgatc
     3481 atcgggcggg tgcatgtcgc ctatctgccg catcaccggg tcgtcggcat cagcaagctg
     3541 gcccgcgtgg tggatgccta tgccaaacgt ttgcagatcc aggaaaagct gacggcccag
     3601 atcgccaaca cgatccagga cgtgctggaa ccgcgcggcg ttgccgtggt gatcgaggcc
     3661 gcgcaccagt gcatgacgac ccgcggcgta cacaagcccg gcgtgacgat ggtcacgagc
     3721 cgtatgctgg gcgccttccg cgacaatgcg gcgacgcggc gggaactgtt ggcgatgatc
     3781 gggaaccgcg gttccaccat catgggcgaa tagagcctgc tggctgagcg aatgaagccg
     3841 gctggctggg ctccgcccgc gcagcttgac tgacgagagg ccgcccgaga cgaccttccc
     3901 ggccttatcg catcgtcggt tgatccagcg gggcctgctc gaccggggtg tccggggcgg
     3961 cgccttcggt tagccagttc ttcaggttgc gacggatctg gtactcgaac tcgacgttca
     4021 tgtcggccat catccccttg gtcaggtcgt acagcaccgg ccggatgttg cgaatttggc
     4081 cgctgtggcg cgactgcacc cgtgcctcgg cggtgcccaa ttgggctccg gcatcgtcca
     4141 gtatggcgag cgatacggcc atggatcctg tcacgatatc ggcctcgcgc atcagcgacg
     4201 catcaaggat ggtgaaaatg cctttgttgg cggtgccaaa cgcctggagc cggtcgtttg
     4261 ccatcgccct cagtgcctcg atcggcggca ccggatcttg attgctgacg tcgggtgcga
     4321 cgccagccgg gataaatcgt tgctggatct cgatcgccgc gacgttcaac tggattggcg
     4381 gcagatagtt gtagcgtaac gggtcgaaaa ccggctccgg ttccccaccg cacgcggcaa
     4441 cggcgagtgg cagcaacagc atgaggcggc gggtcgggtt gttggtttgc atgaaggcgc
     4501 tgcgtccctt gctggtcccg gtggatgaat tgccggaagg cttcggcgtg atctgatggg
     4561 ccgatttacc tgaccctcgg gcaccaggcg gccgatacaa gagccgccgt cgaagcagcg
     4621 ttggctagaa gcattcgcag gctgccgcct atcgggcacg aacctgagtt ggctcgcctg
     4681 cgaaccgcgg ctcatccggg ctttggatca gagggcattt gtccctgtac ggcctgacga
     4741 ggaaaccgga aatcgtaatt gcagaattcg caggtcatga cgatgtcgtc atcgatcgcc
     4801 atatggtcca ggtcgtccgc cgggaagccc tccaggatgc tggcgaggcg cgcgcgcgaa
     4861 cagcggcagc cgaacgacaa cgcccgggcg cgttcgctgg cgacgccctc ggtatggaac
     4921 aaccggtaaa gcagccgttg gctcggcagg ttgtcgtcca acaattcccg ttcggtcagg
     4981 gtggcggcca gggtcgtggc ggtgtgccag ctttcttcct gcgccgcctc
//
LOCUS       DASWQN010000060         1736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_5641, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000060 DASWQN010000000
VERSION     DASWQN010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5641"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VGC82_00995"
     CDS             complement(<1..775)
                     /locus_tag="VGC82_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0421881.1"
                     /translation="MSQALNGIRIIDMTHNQAGPACAQILGFLGADVIKLEEPKGGDV
                     ARTNLRDRPDSDSLFFLILNANKRSVTLNLKTEDGKALFRRLIAESDVLLENFGPGAL
                     DRLGLGYETLAKLNPKLIYATIKGFGTYGPYSGYKSFEPIAQAMGGAMAVTGFPENPP
                     TVVFPAIGDSGTGMHMAIGILAALQQRHTSGKGQHVEVSMQDAVVNLIRVSLRDHQRY
                     GHPPPRVGNQLGRVVPGTTFACSPGGPNDYVYIFAQPQMW"
     gene            complement(847..>1736)
                     /locus_tag="VGC82_01000"
     CDS             complement(847..>1736)
                     /locus_tag="VGC82_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0421882.1"
                     /translation="VFTGAPLGGFLGGQLVAQLLPIYGWTVIFAIGGVLPLLLIPVVL
                     FWLPESPRFLLKKGRLTERSRQLLQYLKIDTEQTGHPVDVVTGNPVAGLFRDGLAPTT
                     VLVWILYFANLISLYLIGYWLPTVLNMSGLSPADSVFAASLRDAGPLVSIFLLAPLSQ
                     RFSPQSVLAFSLAAGIVAIGAVGLLNLPYVALLATIFLIGCCTTGSMTVINGMTAALY
                     PARVRNTGMGWALGIGRLGGIGGPWLGGLLLGLGWPPRQIFLFACVTALVAAIAVLLL
                     RSQARRVGSQPILRRALGS"
BASE COUNT          357 a          565 c          524 g          290 t
ORIGIN      
        1 tccacatctg cggctgagca aatatataga cgtaatcgtt cggaccgcct ggcgaacagg
       61 cgaaggtcgt gccgggaacc acgcgaccga gttgattgcc gacgcgcggc ggcggatgtc
      121 cgtagcgctg atggtcccgc aggctgacgc ggatcaggtt caccacggca tcctgcatcg
      181 acacctcgac atgctgccct ttcccgcttg tatgccgttg ctgcagcgcc gcgaggatcc
      241 caatcgccat atgcatgccg gtgccggaat cgccgattgc cggaaacacc acggtcggcg
      301 gattttccgg aaagccggta acggccatcg caccgcccat cgcctgggca attggctcga
      361 agcttttgta cccgctgtac ggcccgtacg tcccaaaacc cttgatcgtc gcatagatca
      421 gcttcgggtt cagtttcgcc agcgtctcgt accccagtcc caggcgatcc agtgccccag
      481 gcccgaaatt ctccagcagt acgtcggatt cggcgatcag acggcgaaac agcgccttgc
      541 cgtcctcggt cttcaggttc agcgtcacgc ttcgcttgtt ggcgttcagg atcaggaaaa
      601 acaggctgtc gctgtccggc ctgtcgcgca ggttggttcg ggcgacgtcg ccgcccttgg
      661 gctcctccag cttgatcacg tcggcgccca ggaaacccag gatctgtgca caggctggac
      721 cggcctggtt gtgcgtcatg tcgataatgc gaatgccgtt caacgcctgg ctcatgatta
      781 tttccctttt tcaggcttcg acgaatgcgg ctgccgttcc gagcgtcatc ccgatcatcg
      841 aatcgatcag ctccccaaag cgcgccgcag gattggctgc gaaccgacac gccgggcctg
      901 agaccgcagc agcagtacgg cgatcgcggc gaccagtgcc gtgacgcagg cgaacaggaa
      961 gatttgacgc ggaggccagc ccaggcccag caacaagcca ccgagccacg ggccgccgat
     1021 gccacccagg cggccgatgc caagtgccca gcccattccg gtgttgcgca cgcgcgccgg
     1081 atacagcgcc gcggtcatgc cgttgatgac cgtcatgctg ccggtcgtgc agcagccgat
     1141 caggaagatc gtcgccaaca gcgcgacgta gggcaaattc aacagcccga ccgcgccgat
     1201 cgcgacgatg ccggcggcaa ggctgaaagc cagcacgctc tgcggcgaga agcgctgact
     1261 gagcggcgcc agaaggaaaa tgctgaccag cggtccggcg tcgcgcaaac tggccgcgaa
     1321 caccgaatcc gccggggaca gtcccgacat gttcagcacc gttgggagcc agtagccgat
     1381 gagatacaag ctgatcagat tggcgaagta caagatccag actagcaccg tggtcggcgc
     1441 aaggccatcc cggaacagtc cggcgaccgg attgccggtg acgacatcca ccgggtgccc
     1501 ggtctgttct gtgtcgatct tcaaatactg caacaactgt cgtgagcgct ccgtcaggcg
     1561 tccctttttc agcaggaacc ggggcgactc cggcagccag aacagcacca ccgggatcaa
     1621 gagcagcggc aaaacgccgc caatggcaaa gatcaccgtc cagccgtaga tcggcagcaa
     1681 ctgtgccacc agttgcccgc cgagaaagcc gccgagggga gcaccggtga aaacga
//
LOCUS       DASWQN010000061         4885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_5670, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000061 DASWQN010000000
VERSION     DASWQN010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5670"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VGC82_01005"
     CDS             complement(<1..589)
                     /locus_tag="VGC82_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421883.1"
                     /translation="MNTKRRGVLVAVILGAGLIAGAGTLSAQQASQPLSAAQIAQILG
                     SQDRTAADRTNDLRRKPELMLAFIGIRPGIVALDLSAAGGYTTELLARAVGPSGIVYG
                     QSRPPDAGRTATPPAAPEGNSHPPATPSAAPATPTSAPRPSTVALAERDKNLRNAGVP
                     AAPIVAVSRPFEDPVPPELASDRIDLVTLMFNYHDL"
     gene            complement(676..1512)
                     /locus_tag="VGC82_01010"
     CDS             complement(676..1512)
                     /locus_tag="VGC82_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0421884.1"
                     /translation="MSYETIVVKPATVAIGAEVSGVDLSRPLGNQQFQEVHDALMAHQ
                     VLFFRDQKLTLDQHEAFGRQFGELAIHPNTPGPKGHPAILPIHADANSKRIAGERWHS
                     DVSCDPEPPMGSILNLKVVPPVGGDTIFASMYAAFDALSSGMKTYLSALTAIHDGSMN
                     YSRTNRLIGAEGPKTFPRAEHPLIRTHPVTGRHAIYVNPVFTMAIKDVPENESAAILE
                     FLYEHCGRPHWQVRFRWEPDSVAFWDNRCVQHIAMWDYFPDVRSGFRVTIKGDKPFYR
                     PH"
     gene            complement(1646..2731)
                     /locus_tag="VGC82_01015"
     CDS             complement(1646..2731)
                     /locus_tag="VGC82_01015"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEY0421885.1"
                     /translation="MRSWIQHSRGRHVRQARVGLGDLREEHISRQGFFGPVAMIYREH
                     GPNEIVRVEGTLAPRRVVDTTRVEPSDMTDPRGAPEVLLSNPDVSVAISRRTATMPYA
                     YRDTDGDLLYFVHKGTGQFATEFGRILYEPGDYVMIPKGITFCVIPDAEDSHMLVVES
                     PAPLALTEHRQVGRHMPVDTTVLVLPELHDYRWPRQDEYELRVKHGGAHSSIFYRNNP
                     LESVGWKGDLFPYKLNIRDIIPISSDRIHVAPSAWCTFEAAGFVVITFVPQIAVSDLN
                     AEELPSYHRNVDNDESVFVHHDESSSRQPGTLSHTPQGILHGANEAAREAFQKRRTPG
                     MRRTLCGVSVDTDRPLSVSAAYHRLAE"
     gene            3104..4054
                     /locus_tag="VGC82_01020"
     CDS             3104..4054
                     /locus_tag="VGC82_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEY0421886.1"
                     /translation="MQPTLAFAFGSVLALTLSLTPALAQGKKELALVTNAAADFWTIA
                     KRGVEKAQKEHPDYSMQVLVTGQATAAEQRRELDNLLARGVAGVSISAIDPKNSTEEF
                     NKVAAKAVLFTTDSDAPQSNRVAYIGTDNVAAGRQAGAEIKKALPGGGKVMMFVGTMD
                     ADNARERVQGIKDVLAGGNIQIVDIRTDGVDFAKAKSNVQDTLAKGGVDCLVGLYSYN
                     TPQIYSAVKEAGKTGTIKIVGFDEDPQTLRGVADGTIQSTIVQQPYEFGYQSMIDMIK
                     YIGGDRSFIPPNKLIIIPTQVIEKSNVAAFQDSMKQLLKK"
     gene            4071..>4885
                     /locus_tag="VGC82_01025"
     CDS             4071..>4885
                     /locus_tag="VGC82_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0421887.1"
                     /translation="MTSGSLLELAGITKTYPGVTALDEITLSVGRGEVLGLIGENGAG
                     KSTLMKILGGIVAPTAGTIHIDGQAHAMFSVNQSMRAGIAFVHQELNLFDNLDVAANI
                     GMGREPLSFGKLMLINRQRLRDRVRPLLDRLGADFGPDTPVERLSIAQRQLVEIAKAL
                     SIDARIIIMDEPTSSLTLQETERLLRVIAELKADGVSIIYISHRLGEVKACADRVACL
                     RDGRLVGELAREDIDHAAMIRLMIGRDLKSLYIPPAVEPRDGGCDLRAVVSQG"
BASE COUNT          944 a         1496 c         1544 g          901 t
ORIGIN      
        1 cgaggtcatg gtaattgaac atcaaggtca cgaggtcgat gcggtccgac gccagttccg
       61 ggggaaccgg atcctcgaat ggccgggaca ctgcgacgat cggcgcggcc gggacgccag
      121 cgtttcgcaa attcttatcg cgttcagcca aggcgacggt ggacggacgt ggcgcgctcg
      181 tcggcgtcgc cggcgccgcg cttggcgtgg ccggcggatg gctgttgccc tccggggcgg
      241 caggaggcgt ggccgtacgg ccggcgtcgg gcggccggct ctggccgtaa acgatgcccg
      301 agggcccgac cgctcgggct agtaattccg tagtatagcc gccggccgca ctgagatcga
      361 gtgcaacgat accagggcga atcccgatga aggccagcat tagctccggc ttgcggcgca
      421 gatcattggt gcgatccgcc gcagtgcgat cctggctccc caggatctgt gcgatctgcg
      481 ccgccgaaag gggctgcgaa gcctgctgcg ccgacaacgt gcctgcgccc gctatcaggc
      541 cggctcctaa aatgactgca accagcacgc ctcgccgctt ggtgttcatt ggcttgtcct
      601 tcgcgttagt tgactgacgg ccaaccatgt ccccggcagg cagcgccatc tcatggaaca
      661 catcaggtga ggccgttagt gcggccgata aaatggcttg tcccccttta tcgtgacgcg
      721 aaagccggac cgcacgtcgg ggaaatagtc ccacatggcg atgtgctgca cacatcgatt
      781 gtcccagaag gccacgctgt ccggctccca ccggaaacgc acctgccagt gtggccgccc
      841 gcaatgctcg taaagaaact ccaggatcgc cgcgctttcg ttctccggga cgtccttgat
      901 cgccatggtg aacaccgggt tcacgtagat cgcatggcgg cccgtcaccg ggtgcgtgcg
      961 gatcaaggga tgctccgccc tcgggaacgt cttcggtcct tccgcgccaa tcaggcggtt
     1021 ggtgcgactg tagttcatcg acccgtcatg gatcgccgtc agcgcggaaa gataggtctt
     1081 cattcccgag ctcagcgcgt cgaaggcggc gtacatgctg gcaaagatcg tgtccccgcc
     1141 cactggcggc acaactttca ggttcaggat ggaacccatc ggcggttcgg gatcgcagga
     1201 gacatccgag tgccagcgct caccggcgat ccgcttcgag ttggcgtcgg cgtggatcgg
     1261 gagaatggca gggtgcccct ttggccccgg cgtgttggga tggatggcga gttccccgaa
     1321 ctgcctgcca aacgcctcgt gctggtcgag cgtcagtttc tgatcccgga agaacaacac
     1381 ctggtgcgcc atcaaagcgt cgtgtacttc ctggaactgc tggttgccaa gagggcgtga
     1441 caggtcaacg cctgaaacct cggcgccgat tgcgacggtg gccggcttca cgacgatagt
     1501 ctcgtaactc atgttgcgtt tcctcgtttc gttcggggct tgagcaggcg cgtccggtat
     1561 ggcgagccgt cgcatggtgc tgaccccagc accttcatat ccgcttcaca accggaatcg
     1621 cgcgtactgg ctttcgcgat cacgcctact ccgccaggcg gtgataggcc gccgagaccg
     1681 acagtggccg atcggtatcg acgctgaccc cgcacagcgt gcgccgcatg ccgggggtgc
     1741 gccttttctg aaaggcttcc cgcgccgcct cgtttgcgcc atgcaggatg ccctgtggcg
     1801 tgtgtgatag ggtcccgggc tggcgggaac tggattcgtc gtggtggacg aacacgcttt
     1861 cgtcgttgtc cacgttgcga tgataggacg ggagttcctc ggcattcaga tcagatacgg
     1921 cgatctgcgg cacgaaggtg atcaccacga aacccgctgc ctcgaacgtg caccaggccg
     1981 agggcgcgac atgaatgcga tcggagctga tcgggatgat gtcccggatg ttcagtttgt
     2041 acggaaacag atcgcccttc cagccaaccg actccagcgg attgttgcgg tagaatatgg
     2101 agctgtgcgc cccgccatgc ttgacccgca attcgtattc atcctgtcgc ggccagcgat
     2161 aatcgtgcag ttccggcagc accagaacgg tggtatcgac cggcatatgg cggccgacct
     2221 gccggtgttc ggtcaatgca agcggcgccg gcgattcgac gaccagcatg tggctgtcct
     2281 cggcatcggg gatcacgcaa aaagtaatgc ccttggggat catcacgtag tcgcccggtt
     2341 cgtagaggat gcgtccgaac tcggtggcga actgcccggt gcccttgtgc acgaaataca
     2401 acaggtcgcc gtcggtgtcg cgataggcgt acggcatcgt ggccgtgcgg cggctgatcg
     2461 cgacactgac atccgggttg gataacagca cctcgggcgc gccccgcgga tcggtcatgt
     2521 cggacggttc gacccgcgtg gtatcgacaa cgcgccgcgg cgcaagcgtg ccttcgaccc
     2581 gcacgatctc attcggacca tgctctcgat agatcatcgc gaccgggccg aaaaaacctt
     2641 ggcggctgat gtgttcctcg cgcaagtcgc caagtccgac cctcgcctgg cggacatgcc
     2701 gccctctgct gtgctggatc caggatcgca cgatacggca ctcctttctg atgttcctcc
     2761 agggtcggca cggggcgccg gcgtgcatcg gcatggcacc cgcgcctgaa atcggggggc
     2821 ttgaaatcgg ggggggccga aatcgggcgg ccgaaatcgg gccgcacgta ctttgcgccg
     2881 ctttacatga aggtgtcgag aacaggcggt agcgtcggta ggtcgggcgt cggtgatgac
     2941 cgggccgatc cgcaaccgac caaggggcag tcgcgtccgc gcgggtcggc cccgaatgtc
     3001 gttgccgata tgtgaagcaa gtgagtcacc caacgcggcg attgccggga agcgcgaatg
     3061 caccttaaat gctgggaacc ggcaacgtct caggaaacaa aatatgcaac cgaccctcgc
     3121 attcgcgttc ggcagcgtgc ttgccctcac gctgtccctg acgccggcct tggcccaggg
     3181 taagaaagag ctcgccctcg tcaccaacgc cgccgccgat ttctggacga ttgccaagcg
     3241 cggggtcgaa aaggcccaga aggagcatcc cgattacagc atgcaggtgc tggtaaccgg
     3301 ccaggccaca gccgccgaac agcggcgaga attggataac ctgctggccc gaggtgtcgc
     3361 cggcgtctcg atttcagcca tcgacccgaa gaattccacc gaggagttca acaaggtcgc
     3421 ggcgaaagcc gtcctgttca ccacggacag cgatgcaccg cagtcgaatc gggtcgccta
     3481 tatcggcacc gacaacgtcg ctgccggacg gcaagcgggt gccgagatca agaaggcgct
     3541 tccaggtggc ggcaaggtca tgatgttcgt cggcaccatg gacgcggaca acgcgcgcga
     3601 aagggtgcag ggcattaaag acgttctggc cggcggcaat atccagatcg tggacatccg
     3661 caccgacggc gtcgacttcg ccaaagccaa gagcaacgtg caggatacgc tggcgaaagg
     3721 cggcgtcgat tgtcttgttg gcctgtattc ctacaacacg ccgcagatct actcggcggt
     3781 gaaggaagcg ggaaaaaccg gcacgatcaa gatcgtaggc ttcgacgagg atccgcagac
     3841 cctgcgcggc gtcgctgatg gcaccattca gtcaaccatc gtgcagcagc cttacgaatt
     3901 cggctaccag tcgatgatcg acatgatcaa atacatcggc ggcgacaggt cgttcattcc
     3961 gcccaacaaa ctgatcatca tcccgaccca ggtgatcgag aaatcgaacg tcgcggcgtt
     4021 ccaggattcg atgaagcagc ttttgaaaaa atagcctggg caacggaacc atgacgtccg
     4081 gcagcctgct tgaactagcc ggcatcacca agacttatcc cggcgtcacc gccctggacg
     4141 aaatcaccct gtcggtcggc cgtggcgaag tcctcggcct gatcggcgag aatggcgccg
     4201 gcaaatcgac gctgatgaag atcctcggcg gcatcgtggc gccgacggcc ggcacgattc
     4261 atatcgacgg ccaggcgcac gcgatgttca gcgtcaacca gtcgatgcgc gccgggatcg
     4321 ccttcgtcca ccaggagctc aacctctttg acaacctcga tgtcgccgcc aatatcggca
     4381 tgggaaggga accgctcagc ttcggcaagc tgatgctgat aaaccgccag cgcctgcgcg
     4441 accgcgtaag accgttgctt gatcgcctcg gtgctgattt cggccctgat actccggtcg
     4501 aaagattgtc catcgcccag aggcaactgg tcgagatcgc caaggcgctg tcgatcgacg
     4561 cgcgcatcat cattatggac gagcccactt ccagcctgac gctgcaagaa accgagcggc
     4621 tgttgcgggt gatcgccgaa ttgaaggcgg atggcgtgtc gatcatctac atctcgcacc
     4681 gcctgggtga ggtgaaggcc tgtgcggatc gtgtggcctg cctgcgcgac ggacgactcg
     4741 ttggcgaact tgcgcgcgaa gacatcgatc atgccgccat gatccggctg atgatcggac
     4801 gcgacctgaa gtcattgtac attccgccgg ccgtcgaacc tcgggatggc ggatgtgatt
     4861 tgcgtgccgt ggtatcccaa ggctt
//
LOCUS       DASWQN010000062         7623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1457176, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000062 DASWQN010000000
VERSION     DASWQN010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457176"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VGC82_01030"
     CDS             <1..246
                     /locus_tag="VGC82_01030"
                     /inference="COORDINATES: protein motif:HMM:NF018464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HEY0421888.1"
                     /translation="RCTVRTETGFIGHAYVAGRDEKRAELAALTDALMQDPDRTVEIE
                     RNIIAPLEWEQNARATRRAEKAAATRVQFFAMQTMRT"
     gene            243..815
                     /gene="phnH"
                     /locus_tag="VGC82_01035"
     CDS             243..815
                     /gene="phnH"
                     /locus_tag="VGC82_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HEY0421889.1"
                     /translation="MSSNLSPGFADPVTSAQACFRAVLDAMAHPGRIKTAPEVTAPAP
                     LCAASGAVLLTLVDPETPLWLDPEAATARAWIGFHTGAPLEVATRATFAMALSLPDLG
                     ELPNGSDEAPETSATVILQVRSLTAGQHYWVTGPGLREPADLLVDGLPPDFADIWQRN
                     HAQFPRGVDLILCAGNTLTALPRGVTVRKV"
     gene            819..1889
                     /locus_tag="VGC82_01040"
     CDS             819..1889
                     /locus_tag="VGC82_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HEY0421890.1"
                     /translation="MAYVAVKGGEQAIDNAHAWLAEERRGDPSTPALSIPQIQQQLGR
                     SVDRVMAEGSLYDRELAALAIKQAQGDLIEAAFLLRAYRTTLPRFAHSRPVETAKMRI
                     ERRVSAIFKDLPGGQVLGPTYDYTHRLLDFGLLADEAIASGAAGEADRTTMPRVPDIL
                     GHEGLLEPDAASDDEPGDLTREPLSFPADRDVRLQALARGDEGFVLGLGYSTQRGYGG
                     SHPFAGEIRMGEVSVEIEPAELGFAIDIGDLVLTECQMVNQFAGSKTVPPQFTRGYGL
                     VFGFGERKAMAMALVDRAMRAGELGEDVVAPAQNVEFVLHHSDNIEATGFVEHLKLPH
                     YVDFQSELQNVRRMRAQAEAAE"
     gene            1886..2752
                     /locus_tag="VGC82_01045"
     CDS             1886..2752
                     /locus_tag="VGC82_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HEY0421891.1"
                     /translation="MKGYNFAYLDEQTKRMIRRGLLKAVAIPGHQVPFGSREMPLPYG
                     WGTGGIQVTASILGAGDVLKVIDQGADDTTNAVSIRRFFARTAGVATTTATTEATVIQ
                     TRHRIPEKQLREDQIIVYQVPQPEPLFRLEPSRVETKRMHGLADYGLMHVKLYEDISR
                     YGQISISYDYPVMVNERYLMSPSPIPAFDNPKMDMMPALQLFGAGREKRIYAIPPWTR
                     VRSLDFHDHPFRPINAPQPCSLCGAADAYMDEIVTDNVGGRLFVCSDTDHCAERRAAG
                     HVGSEGLPEAAE"
     gene            2782..3558
                     /gene="phnK"
                     /locus_tag="VGC82_01050"
     CDS             2782..3558
                     /gene="phnK"
                     /locus_tag="VGC82_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HEY0421892.1"
                     /translation="MCEPTLLSATGLDLNFAGITALDDVDVDLWPGEILAIVGESGSG
                     KTTLLNVLSGRAAPDRGIVRYRDPAGQSHDVHAMPAPALRTLHRSDWGFVHQDPRQNL
                     RMAVSAGGNVGERLMAQGARHYGNIRDAALDWLAQVEIDADRIDDLPRTFSGGMLQRL
                     QIARTLVTHPRLVFMDEPTGGLDVSVQARLLDLIRSLVTRLHIAVVLVTHDIAVARLL
                     AHRIAVMQRGRIVETGLTDQVLDDPSHPYTQLLVSSVLTA"
     gene            3555..4259
                     /gene="phnL"
                     /locus_tag="VGC82_01055"
     CDS             3555..4259
                     /gene="phnL"
                     /locus_tag="VGC82_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HEY0421893.1"
                     /translation="MSDAIMLQTRGLAKSFTLHLRGGLLIQVLSNVDLDVRGGECVVL
                     SGPSGAGKSTLMRSLYGNYRAESGSILIRHRGQTVDIATVDPRTILAVRHETLGYVSQ
                     FLRVVPRVPALDVVAEILLARGIDQPTAREKARALLLKLNIPSRLHAIPPATFSGGEQ
                     QRVNLARGFIAGHPILLLDEPTASLDAENRAVVIDMVRDAKANGTAIVAICHDADVRD
                     AIADRVFTLTPLQDAA"
     gene            4256..5395
                     /locus_tag="VGC82_01060"
     CDS             4256..5395
                     /locus_tag="VGC82_01060"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HEY0421894.1"
                     /translation="MTHETVLTNAILVLRDATLFGTIVMHGRTIADMQTGSSRVAGAI
                     DMDGDYVIPGVVDVHTDNLERQVQPRSLARWPSRSAMVAHDAQCAAAGVTTVLDALCL
                     GDLGFDKDRIQTFQDGVVDLDALTAAGLLKSDHFLHLRCEVPAEGVLELLDPVADHPL
                     VRMISLMDHSPGVGQYADMDFYRKLRRQGGLDEATLERRIGEMQAQRVRLRDANRRAL
                     LDRVRGTSIALASHDDRTEEEIAENAADGIRISEFPVSMIAAVAAKKAGMEVIAGAPN
                     IVRGGSHSGNVAAADLLQAEAVDAFASDYVPSSLVEAAFQCAREKGINLPQAIAMISD
                     HPARMSGLPDRGRLENGLRADVVRVRLHGSLPIVRQVWRAGERII"
     gene            5395..6072
                     /locus_tag="VGC82_01065"
     CDS             5395..6072
                     /locus_tag="VGC82_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HEY0421895.1"
                     /translation="MSPAARVAIYYAPLPDDPLAALGASWLGRDPATNAPVPQPMLHG
                     IAEITAEPRTYGFHATLKPPMRLAGDWAEFMAAVRAAAAAIAPFDLPPLAVADLRGFL
                     ALRETRPCTALQALADACVVQLDPFRVPPSEDELTRRRTARLTPEQDAMLMRWGYPYV
                     LGTWFFHMTLTRRLSDIEKSAFLPAAKAWFAPALTFPRRVEDICVFTQTTPGAAFTLA
                     ERTKLRG"
     gene            6502..7272
                     /locus_tag="VGC82_01070"
     CDS             6502..7272
                     /locus_tag="VGC82_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421896.1"
                     /translation="MLQPGVTLNEAVTAPLLAAGWQCGAVTFKGTDLNPFRYVMPGPA
                     DDANHVAYFTEPRAPRGTTRIEQANATFGWADGKPFIHCHAAWSEPDGRRRGGHILPL
                     ETIVAALGEATAWGFRDVRIATSLDLETNFTLFQPSGFQPSGAGQSTGAGLVARIKPN
                     EDIITSMETIARDYGICNAAVRGSLIGACFKDGKRVADLATEVLVREGYIRDGKACLD
                     LLVVDMRGQVHAGWLQRGENAVCITFDLIMEDQGSTTF"
     gene            7398..>7623
                     /locus_tag="VGC82_01075"
     CDS             7398..>7623
                     /locus_tag="VGC82_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421897.1"
                     /translation="MEDDDTALTQLIAVLDILSHIARMMHPRRLTQLIAQIGDRDAAL
                     GVALNGLDPDSPIDRQIEIAATLASRTCAGL"
BASE COUNT         1357 a         2552 c         2394 g         1320 t
ORIGIN      
        1 cgttgcacgg tgcgaaccga gaccggcttc atcggccacg cctacgtcgc cggacgcgac
       61 gaaaagcgtg ccgaactggc cgccttgacc gatgccttga tgcaggaccc cgaccgcacg
      121 gttgagatcg agcgtaacat aatcgccccg ctcgagtggg agcaaaatgc ccgtgcgacc
      181 aggcgcgccg agaaggccgc ggcaactcgg gtgcagttct tcgccatgca gacgatgcgg
      241 acatgagcag caacctctcg cccgggttcg ccgatccggt caccagtgcc caagcgtgct
      301 ttcgcgccgt actcgatgcc atggcccacc cgggacgcat caagacggca cccgaggtca
      361 ccgccccggc acctctgtgc gctgcctcgg gcgccgtgct gttgaccctg gttgacccgg
      421 agacaccgct ttggctggat cccgaagccg ccacggcgcg cgcctggatc ggcttccaca
      481 ccggtgcgcc gcttgaagtc gccacccgtg cgacgttcgc catggcactg tccctgcccg
      541 acctgggtga gctcccgaac ggcagcgatg aagcgccgga aacctcggca accgtgatct
      601 tgcaggtcag gtccctgacg gccgggcaac attactgggt gaccggaccc ggcctgcgcg
      661 aacccgcgga tctgctggtg gacggcctgc cgccggattt cgccgacatc tggcaacgca
      721 accacgccca gttcccgcgc ggcgtcgatc tgatcctgtg cgcgggcaat acgctgaccg
      781 cgctgccgcg cggcgtcacc gtgcgaaagg tctgaacgat ggcttatgtc gcggtcaagg
      841 gcggtgaaca ggcgatcgac aacgcccacg cgtggcttgc cgaggaacgc cgaggcgatc
      901 cctccacccc cgccctttcc attcctcaaa ttcagcagca actgggccgt tcggttgacc
      961 gcgtcatggc cgagggctcc ttgtacgacc gggaactggc tgccctggcg atcaagcagg
     1021 cgcaaggcga cctgatcgag gccgccttct tgttgcgcgc ctaccgcacc acgttgccgc
     1081 gcttcgccca ctccagaccg gtcgagaccg cgaagatgcg gatcgagcga cgggtgtcgg
     1141 cgatcttcaa ggacctgccc ggcggacaag tcctggggcc gacctacgat tacactcatc
     1201 gcctgctgga tttcgggctg ctggccgacg aggccatcgc gagcggagcg gccggcgagg
     1261 ccgatcggac gacgatgcca agggttcccg acatcctggg tcacgagggc ctgctggagc
     1321 cggacgcggc atcggacgat gaacccggcg acctgacccg ggagcccttg tcctttcccg
     1381 cggatcggga tgtgcgcctg caggccctgg cccgtggcga cgagggcttc gtcctggggc
     1441 tgggctactc cacccagcgc ggctacggcg gcagccaccc gtttgccggc gaaatccgca
     1501 tgggcgaggt atcggtcgag atcgaaccgg cggagcttgg ttttgccatc gacatcggcg
     1561 acctggtcct gaccgaatgc cagatggtca accagttcgc aggctcgaaa accgttcctc
     1621 cgcagttcac caggggctac ggcctggtgt tcggctttgg cgagcgcaag gcgatggcga
     1681 tggccctggt cgatcgcgcc atgcgcgccg gagaacttgg cgaagacgtc gtcgccccag
     1741 cgcaaaacgt cgaattcgtg ctccaccatt cggacaatat cgaagcgacc ggtttcgtgg
     1801 agcacctgaa actgccgcac tacgtcgatt tccagagcga gttgcaaaac gtccggcgga
     1861 tgcgcgcgca ggcggaggcc gccgaatgaa gggctacaac ttcgcctatc tggacgagca
     1921 gaccaagcgg atgatccggc gcgggctgct gaaggcggtt gcgatccccg gtcaccaggt
     1981 gccgttcggc tcgcgggaga tgccgttgcc gtacggctgg gggacgggag gaatccaggt
     2041 gaccgcgtca atccttggcg ctggcgatgt tctgaaagtg atcgaccagg gtgccgacga
     2101 caccaccaat gcggtatcca tccgccgctt cttcgcccgc accgccgggg tggcgacgac
     2161 aaccgcgaca accgaggcga cggttatcca gacgcggcac cgcatcccgg agaagcaact
     2221 gcgcgaggac cagatcatcg tctatcaggt gccgcaaccc gaaccgctat ttcgtctgga
     2281 gcccagccgc gtcgagacga aacgaatgca cgggctcgcg gattacggcc tgatgcatgt
     2341 aaagctgtac gaagatattt cccgctacgg ccagatatcc atcagctacg actacccggt
     2401 gatggtgaac gaacgttact tgatgtcgcc cagcccgatc ccggcgttcg acaatccgaa
     2461 gatggacatg atgccggcgc tgcagctttt cggcgccggc cgggaaaaac ggatctacgc
     2521 gatcccgccc tggacccggg tcagatctct cgatttccac gaccatccat ttcggccgat
     2581 caatgcgccg cagccatgca gtctgtgcgg cgcggccgac gcatacatgg acgagatcgt
     2641 caccgacaat gtgggtggcc gcctgtttgt ctgctcggac accgatcatt gcgccgaacg
     2701 ccgcgccgcc ggccatgtcg gcagcgaagg cttgccggag gccgccgaat gatgtccggc
     2761 cccatcgaag ctgcctccgg gatgtgcgaa cccacgcttt tgtccgccac gggcctcgac
     2821 ctgaactttg ctggaatcac cgccctggac gacgttgacg tggacttgtg gcctggcgaa
     2881 atcctggcga tcgttggcga gtcgggctcc ggcaagacca cgcttctgaa cgtgctgtcc
     2941 ggccgcgccg cccccgaccg cggcatcgtc cgctaccgtg atccggccgg ccaatcgcat
     3001 gacgtacacg ccatgccggc accggccttg cggaccttgc accgatccga ctggggcttc
     3061 gttcaccagg acccgcgcca gaacctccgc atggcggtca gcgccggcgg caatgtcggc
     3121 gaacgcctga tggcccaggg cgcgaggcac tacggcaaca tccgcgacgc cgcgctggat
     3181 tggctcgcac aagttgagat cgatgccgac cgcatcgacg acctgccacg cacattctcc
     3241 ggcggcatgc tgcagcggtt gcagatcgcc cggacgttgg tgacgcatcc gcgcctggtg
     3301 ttcatggacg aacccaccgg cgggctggat gtgtcggtcc aggcacggct tctcgacctg
     3361 atccgctcgc tggtcaccag gctgcacatc gccgtcgtgc tggtgaccca cgatatagcc
     3421 gtcgcccggc ttctggcgca ccggatcgcg gtcatgcagc gcgggcgcat tgtggagacc
     3481 gggctgaccg accaggtgct tgacgatccc agtcacccat acacgcagct gctcgttagc
     3541 tcggttttga ctgcatgagc gacgcgatca tgctgcaaac gcgcgggctg gcgaaaagct
     3601 tcacgctcca tttgcgcggc ggcctgctga tacaggtgtt gtccaacgtc gatctcgatg
     3661 tccgcggcgg cgagtgcgtc gtgttgtccg gcccgtccgg agccggcaag tccactttga
     3721 tgcgcagcct ttacggcaac taccgcgccg aaagcggcag catcctgatc cgccaccgcg
     3781 ggcagacggt cgatatcgcg accgtcgacc cgcggaccat tttagcagtt cgccacgaaa
     3841 ccctgggcta cgtcagccag ttcctgcgcg tcgtcccacg cgtgccggcc ctggatgtgg
     3901 tcgccgaaat actgctggct cgcggcatcg atcaaccgac cgcgcgggaa aaggcccgcg
     3961 cgctgttgct gaagctcaat attccgtccc gcctgcacgc gatcccgccc gccactttct
     4021 ccggcggcga gcagcagcgc gtaaatctgg ccagaggctt catcgccggc catccgatcc
     4081 tgctgctgga cgagccgacg gcgtcgctcg atgcggaaaa cagagccgtt gtgattgaca
     4141 tggtccggga cgccaaagca aatggcaccg ctatcgtcgc gatctgccac gatgccgacg
     4201 tccgcgatgc catcgcggac cgggtgttca cgttgactcc cctccaggat gccgcatgac
     4261 ccacgagacg gtgctgacca acgcgatcct ggtgcttcgc gacgcgacgc tgttcggcac
     4321 catcgtgatg catggccgga cgatcgccga catgcagacc gggtcgtctc gggtggccgg
     4381 agcgatcgac atggacggcg actacgtcat tcctggcgtc gtcgatgtcc acaccgacaa
     4441 cctggaacgc caggtgcagc ctcgaagcct ggcgcgctgg ccatcgcgct ccgccatggt
     4501 cgcgcatgac gcgcaatgtg ccgcggccgg tgttacgacg gtgctggatg cgctttgcct
     4561 gggcgacctc ggcttcgaca aggatcgcat ccagacgttc caggacggcg tggtcgatct
     4621 ggacgccctg accgcagccg gactgctgaa atccgaccat ttcctgcatc tgcgttgcga
     4681 agtcccagcg gagggcgtgc tggaactgct cgatccggtc gcggatcatc ctttggtgcg
     4741 catgatcagc ctgatggatc acagtccggg cgtcggccag tacgccgata tggatttcta
     4801 ccgcaagctg cgccggcagg gcggactgga cgaggccacg ctcgaacgcc gcatcgggga
     4861 gatgcaagct cagcgcgtac gcctgcgcga tgccaaccgc cgagcgttac tcgaccgcgt
     4921 gcggggcacc tcgatcgcct tggccagcca tgacgatcga accgaggaag aaatcgctga
     4981 gaacgccgcc gacggtatcc ggatcagtga gtttccggtg tccatgatcg ccgctgtcgc
     5041 cgcgaaaaaa gccggcatgg aggtgatcgc cggtgcgccc aacatcgtcc gcggcggctc
     5101 gcactccggc aatgtcgcgg cagccgacct gctgcaagcc gaagcggtgg atgccttcgc
     5161 ctcggactat gttccgtcca gcctggtcga ggccgccttt cagtgcgctc gtgagaaagg
     5221 catcaacctg ccgcaagcaa tcgccatgat ctccgaccac cccgcccgga tgtcggggct
     5281 gcctgatcgc gggcgcctcg aaaatggcct gcgcgccgat gtggttcgcg ttcgcctcca
     5341 cggtagcctg cccatcgtgc gccaggtgtg gcgcgccggg gaacggatca tctgatgtcg
     5401 ccggctgcca gggtcgccat ctactacgcc cccctgccgg acgatccgct agccgccctt
     5461 ggtgcctcct ggctcggtcg cgatccggcg accaacgccc cggtgccgca gcccatgctg
     5521 cacggcatcg ccgaaatcac cgccgaaccg aggacttacg ggttccatgc gactctgaaa
     5581 cccccgatgc gcctggccgg ggactgggcc gaattcatgg ccgccgtgcg ggccgcggcg
     5641 gcggccatcg ccccgttcga tcttccaccg ctggccgtgg ccgacctgcg ggggtttctg
     5701 gcgctgcggg aaacacgccc ctgcaccgcc ctgcaggcgc tggccgatgc gtgcgtggtg
     5761 cagctcgatc cattccgggt cccgccgtcc gaggatgaat tgactcggcg ccggaccgct
     5821 cgcctaaccc ccgaacaaga cgccatgttg atgcgctggg gctatccgta tgttctaggc
     5881 acatggttct tccacatgac gctgacacgg cgcctgtcgg acatagaaaa atccgcgttc
     5941 ctgccagccg ccaaagcctg gttcgcccca gcgttgacct tcccgcgccg cgtcgaggac
     6001 atctgcgtgt ttacccaaac cacgccgggt gccgccttca ccctggcgga acggacgaag
     6061 ctgcgcggct gagggtttga cgacaaatca cgttgcaagg cgcgtgatcc tgcccagcaa
     6121 cgtgacggtg ggcttcaaac gaagtatgtc ggacttgtcg ccactgcgcg gtctttgctg
     6181 attatgtcgc ggccttgtgc cggccaccca cgacctcgtg ttgagacaag gtcgtgggtg
     6241 accgcgccaa gcacggccat ggcactgtca tcaaacactg cagccttcgt tcaccatgac
     6301 cgagacctga cctcgcgttt gaagcggtta tctccgagcg gaccctgacc gtagcatcac
     6361 gggtttttgc acgttccgcg aagcgcgtgc atgcttggaa gtcaatcgtc gagaatccgt
     6421 atgcgaacgt tgcgacagcc gggcccggcc cacccaagcc gaatcggttg ctttcggggc
     6481 gaaatccaac cgctgcactt catgttgcag cctggtgtca cgttgaacga ggcggtcacc
     6541 gctccgctgc tcgcagcggg ttggcaatgc ggcgcggtaa ctttcaaggg taccgacctg
     6601 aacccattcc gctacgtcat gccaggcccg gcggacgacg cgaatcacgt cgcctatttc
     6661 accgaacccc gggcgccccg cggcaccacg cgcatcgaac aggccaacgc gacgttcggc
     6721 tgggcggatg ggaagccatt catccactgc cacgccgcct ggtccgaacc cgacggccgc
     6781 cgccgcggcg gccacatcct gccgcttgaa accatcgtcg ccgcacttgg cgaggcaacc
     6841 gcgtggggtt ttcgtgacgt tcggatcgca accagcctgg acctggaaac caatttcacc
     6901 ttattccagc cctcggggtt ccagccctcg ggcgccggtc agagtaccgg cgcaggcctg
     6961 gtcgcccgca tcaagccgaa cgaggacatc atcacgtcga tggaaacgat cgcccgcgac
     7021 tacgggatct gcaatgccgc cgttcgaggc agcctgatcg gcgcatgctt caaggatggc
     7081 aagcgggttg ccgacctcgc gaccgaggtt ctggtacgcg aagggtatat ccgtgacggc
     7141 aaggcttgcc tggacctact cgtggtggac atgcgtgggc aagtgcatgc gggttggctt
     7201 cagcgcggcg aaaacgccgt ctgcatcacg ttcgacctga ttatggagga tcagggctcc
     7261 accacgttct aagccgggat caaggcccgg cacagggcga gcgtgcgatc accggagggc
     7321 aattggtaga tcattaagcg tcgatcgcgc ggacctgtaa ggaactggag ctgcgacggc
     7381 aggaaaggca caagccggtg gaagacgacg atacggcttt aactcagctt atcgcggtgt
     7441 tggacatcct gagccacatc gctcgcatga tgcacccaag gcgattgacc caattgatcg
     7501 cgcaaatcgg cgatcgagac gcggcgctgg gcgttgctct caacggcttg gacccggact
     7561 ccccgataga caggcagatc gaaatcgccg ccactttggc atcccgaacc tgcgctggat
     7621 tac
//
LOCUS       DASWQN010000063         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1853332, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000063 DASWQN010000000
VERSION     DASWQN010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853332"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VGC82_01080"
     CDS             <1..515
                     /locus_tag="VGC82_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0421898.1"
                     /translation="TLISVGGLIERKGHHRTIGAMRQLPGFHLMIVGDGPERERLSAL
                     ISAFGLADRVRLLGPRPHKDLPALYGAADASILASSREGWANVLLESMACGTPVVAAN
                     IWGNPEVVRAAAAGVIYEPNTADGIAAAVRRLFSNPPDRAATRAYAEPFSWDETTEGQ
                     LRLFRRVIGR"
     gene            627..1490
                     /locus_tag="VGC82_01085"
     CDS             627..1490
                     /locus_tag="VGC82_01085"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0421899.1"
                     /translation="MVTLTDILETTDLVRVATGFKFTEGPLWHPDGFFYFVDLPSNLL
                     FRMVPGQAPEKVRTTQGGNGTTFDLQGRLLNCEGEGRRVTRADHDGTVSVLIDRFEGK
                     RLNRPNDVICHSDGSIFFTDPSLRVPPAERELDHAGIYRIAPDGSVSLVAKVEYPNGL
                     ALSKDERTLFVANTRWTQYIHALELDTSGNLVRRRIFADMSADGTNGVPDGMKIDESG
                     RVFCTGTGGVWVFEPDGSRIGIIETPEVCANVAFGGPDLRTLLLTASTSVYTMRVKVP
                     GLPHPFGKRAL"
     gene            complement(1485..>1607)
                     /locus_tag="VGC82_01090"
     CDS             complement(1485..>1607)
                     /locus_tag="VGC82_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEY0421900.1"
                     /translation="QGGGHAWFGGSPSGSYTDPRGPDATNEMLRFFLAHPQGQS"
BASE COUNT          280 a          469 c          547 g          311 t
ORIGIN      
        1 cgacgctgat ctcggtcggt ggcctgatcg agcgcaaggg acaccaccgg acgatcgggg
       61 caatgcgcca gcttcccggc ttccacctca tgatcgtggg cgatggaccg gagcgggaac
      121 ggttgtcggc tttgatcagc gcattcggtc tggcggaccg ggtgcggctg ttggggcctc
      181 ggccccataa ggacctgccc gcgctgtatg gggcggcgga tgcgtcgata ttggcttcat
      241 cgcgggaagg ttgggccaac gtgctgctgg agtcgatggc ttgcggcacg ccggtcgtcg
      301 ccgccaatat ctggggcaac cctgaagtgg tacgggcagc cgcggccggc gtgatctacg
      361 agccaaacac ggcggatggc atagcagcgg cggtgcggcg tctgttttcc aatccaccgg
      421 atcgggcggc gacgcgggcc tatgcggaac cattcagctg ggatgagacc acggaaggcc
      481 agttgcgtct gttccggcgg gtgatcgggc ggtagcatca gccggcatcg gcgtgctgga
      541 cgttggtacg aatgcggtgt tgtgttcgct ggtgcacggg ttcaggtcgc gggacatccg
      601 ccgccacaag aaaaacgggg gaaacgatgg ttacattgac cgatattctt gaaaccacgg
      661 acctggttcg ggtggcgacc gggttcaagt tcaccgaagg gccgctatgg catcccgatg
      721 gattcttcta cttcgtcgat ctcccgtcga acctgctatt tcgcatggtt ccaggccagg
      781 ctccggagaa agtccgcacg acgcagggtg gcaacggcac gacgttcgat ctccagggta
      841 ggttgctcaa ctgcgaaggc gaggggcgcc gcgtgacccg cgccgatcac gacggaacag
      901 tctcggtgtt gatcgaccgg ttcgagggca agcggctcaa tcgcccgaac gacgtgattt
      961 gccattccga tggatcgatc tttttcaccg acccgtcctt gcgggtgccg ccggcggagc
     1021 gcgaactcga ccatgccggg atctaccgga tcgccccgga cggctccgtt tcgttggtgg
     1081 cgaaggtcga atatccgaat ggcttggcgt tgtcgaagga tgagcggacg ttgttcgtcg
     1141 ccaatacaag atggacgcag tatatccacg ccttggaact cgacacctcc ggcaacctgg
     1201 tgcgacgccg catttttgcc gacatgtcgg ccgatgggac gaatggcgtt cccgacggca
     1261 tgaagatcga cgaatccgga cgcgtcttct gtaccggcac gggcggcgtg tgggtgttcg
     1321 agccggatgg cagccgcatt gggatcatcg aaacgccgga ggtttgcgcc aacgtcgcct
     1381 tcggcggtcc cgatcttcgc accttgttgc tgaccgcctc gacatccgtc tatacaatgc
     1441 gggtgaaggt gccgggcctg ccgcatccct tcggaaagcg cgcgctatga ctggccttgg
     1501 ggatgcgcca gaaagaagcg cagcatctcg ttggtcgcat ccggtccgcg tgggtcggtg
     1561 tacgatccgg acgggctgcc gccaaaccag gcatggccgc caccctg
//
LOCUS       DASWQN010000064         1974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2381222, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000064 DASWQN010000000
VERSION     DASWQN010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2381222"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..898
                     /locus_tag="VGC82_01095"
     CDS             77..898
                     /locus_tag="VGC82_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421901.1"
                     /translation="MVPLEPEFIVPQDGHDKQDCESRALRRWLERHGRRHARLKPIYL
                     GDDLFSRQPVCEAVRAVDGHFLFVCKPSSHQTIEEYLTGIDLPTLSRKVKRGRERFDY
                     RYRWLSDVPLRGDADAMTVNWLMIEIVNAVGAVTYRNSFITDLEVGPDTVAELAACGR
                     ARWKIENESFNVLKNNGYHLEHNFGHGKVNLSAVFVSLNLLAFAFHTVCDLAEDLWRK
                     AMEKMGARGRFFENLRSITTFLIFPPGTTFWQRWPSPDLRPWPFDHRAIPSIRPI"
     gene            1054..1875
                     /locus_tag="VGC82_01100"
     CDS             1054..1875
                     /locus_tag="VGC82_01100"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0421902.1"
                     /translation="MVLTKDQCDFFWRNGYLAIPSITTSDELVKIRRIYDRLFEDRAG
                     WEDGNYFDFAGYSEKDADFKVPQLQMMTNYAPELFETLYYANAEAASRQLLGHRAKHL
                     FDHGIRKPARTGGETPWHQDIAFLDGDSYFESLTVWMPLQAVNAVNGCMSFVPGSHLG
                     PVVEHRSPGGDNRANGLEAVSPDVSKAVSCPLPAGGATFHHFRTLHYAAGNHSDQLRR
                     VLSWGFGIRRTEPVVSGTFPWIAAKDNERLHRWNSNRVLQRAKQAVVTHLNRFLT"
BASE COUNT          386 a          629 c          591 g          368 t
ORIGIN      
        1 caagcgcggc caggacagga ccgagcactt ccacgcgatg gtgtcggcga cggtcgtggc
       61 acccgatcac aaccgggtgg tgccgctgga gccggaattc atcgtgcccc aggatggtca
      121 cgacaagcag gactgcgaaa gccgggcgct tcggcgctgg ctggagcgtc acggccggcg
      181 ccacgcccgg ctgaagccga tctatctggg cgacgacctg ttctcccgtc agccggtctg
      241 cgaggcggtg cgggcggtcg acggacattt cctgttcgtg tgcaagccgt cgtctcatca
      301 gacgatcgag gaatacctca ccggcatcga cctgccgacg ctgagccgca aggtcaagcg
      361 cgggcgcgag cgcttcgact accgctaccg ctggctgagc gacgttccgc tgcgcggcga
      421 tgctgatgcc atgaccgtca actggctcat gatcgagatt gtcaacgccg tcggcgccgt
      481 tacctaccgc aacagcttca tcaccgacct cgaagtcggc cccgacacgg tggccgaact
      541 ggccgcctgt ggccgggcgc gctggaagat cgagaacgaa agcttcaacg tcctgaagaa
      601 caacggctac catctcgaac acaatttcgg gcacggcaaa gtcaatcttt ccgccgtgtt
      661 cgtctccctg aaccttctgg ctttcgcctt ccataccgtc tgcgatctgg ccgaagatct
      721 ctggcgcaag gcgatggaga agatgggagc ccgcggccgg ttcttcgaaa atctgcgatc
      781 catcaccacc ttcctgatct tcccccctgg gacgaccttc tggcaacgct ggccttcgcc
      841 agacctccgc ccatggccct ttgatcaccg tgctatcccg agtatcagac caatttagaa
      901 ctgctggaga atagtatagc gtggagattg gcatcctccc tacctttcaa atgtgagcgc
      961 ggggcacaga caatgcaact gtcatcgatc agggatgtca ttcatccgcg ccaggttgtt
     1021 cggtcgttgt tgcatccgaa acaagcgccg acgatggttc tgaccaaaga tcaatgcgac
     1081 ttcttctgga gaaatgggta cctggcgata ccgtcgatca ccacgtcgga cgaactcgtg
     1141 aagatccgcc ggatctacga ccggctgttc gaggaccgtg cggggtggga ggacgggaac
     1201 tatttcgact tcgccggata cagtgaaaag gatgcagact tcaaggtccc acaactccag
     1261 atgatgacga attatgcgcc ggagttgttc gagacgctgt actatgccaa tgccgaggcg
     1321 gcgtccaggc agctgctggg tcaccgggcc aagcacctgt tcgatcacgg catccgcaag
     1381 ccggcccgca ccgggggcga gacaccgtgg catcaggaca tcgcgttcct cgacggcgac
     1441 agctacttcg aatccctgac ggtgtggatg ccgttgcagg cagtgaacgc ggtcaatggc
     1501 tgcatgagct tcgtacccgg aagccatctc ggcccggtgg tcgagcaccg cagccccggc
     1561 ggcgacaacc gtgccaatgg cctcgaagct gtctcgcccg acgtgtccaa ggcggtgtcg
     1621 tgcccgctgc cggcgggtgg cgccaccttc catcatttcc gcacgctgca ctacgcggcc
     1681 ggcaaccatt ccgaccaact gcgacgggtg ctgtcctggg gcttcggcat ccgccggacg
     1741 gaacccgtcg tcagcggcac cttcccctgg atcgcggcga aggacaatga gcggctgcac
     1801 cgctggaact cgaaccgtgt cctgcaacgc gccaagcagg cggtcgtcac ccacctaaac
     1861 aggtttctga cctgaagggg ataaggcggc cggcttttcc cttccacctc cccagctccg
     1921 gcgcagtcat atggagatgt cccgatcaat gttgcaaatc ggaggtgagc gttg
//
LOCUS       DASWQN010000065         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_666106, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000065 DASWQN010000000
VERSION     DASWQN010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666106"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /gene="parA"
                     /locus_tag="VGC82_01105"
     CDS             complement(<1..541)
                     /gene="parA"
                     /locus_tag="VGC82_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HEY0421903.1"
                     /translation="MAVVITVAQQKGGTGKTTLAANLAAALAPSRSVALVDIDPQHSL
                     ARWNERRPTTAAKLMFLEVSGWRVTGELDRLKAAYDIVLIDSPPQIDTDARLAIRGAD
                     LVLIPVQPSPPDIWAAEGTLKLAAVEKRKAAMVLNRVPSAGKLRVAIAAQLRQEGKPL
                     LTATIGNRTGFATAFAEGLG"
     gene            693..1133
                     /locus_tag="VGC82_01110"
     CDS             693..1133
                     /locus_tag="VGC82_01110"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0421904.1"
                     /translation="MPTDPPKGEGCYRLAAPRCDQDWHAYHGIRRKVFHLPRPDEPAS
                     PDCHALLLFLEDRPIGAIQVDDLRNEAAALRLVAIDPAFQGGGHGRAMLERAEQFVRG
                     LGCSKAVVYATPEAAGFYQTAGYDEEDWDEICMGGIVQMLKKLD"
BASE COUNT          294 a          521 c          558 g          311 t
ORIGIN      
        1 cacccaggcc ttcagcgaac gcggttgcaa agccggtgcg gttgccgatg gtcgcggtca
       61 gcagcggctt gccctcttgc cgaagttgcg cggcgatcgc cacccgaagt ttaccggcgg
      121 acgggacgcg gttcagcacc attgccgcct tacgcttctc gacagcggcg agcttcaagg
      181 tgccttcggc tgcccagatg tcgggcgggc tgggctgaac cggaataagc accagatcgg
      241 ctcctcgtat agccagcctg gcatcggtgt cgatctgggg cgggctgtcg atcaggacga
      301 tgtcataggc ggccttcagg cggtccagtt cgccggtaac gcgccagccg gacacttcaa
      361 ggaacatcag cttcgcagcc gtcgtcgggc gacgttcatt ccaacgggcc aggctgtgtt
      421 gagggtcgat atcgaccagc gccacgctgc gcgacggtgc cagggcggca gccaaattcg
      481 ccgccagggt cgttttcccg gtgccgcctt tctgttgcgc gactgtgatg acaaccgcca
      541 tgcgatccag ctccgtccca atggtttcat acagttccag aaccggcgat cggacgccag
      601 gacctcgtcc gagggcggct cgcttttccg ccggaccttc cgtatcttcc gagagccgtc
      661 gcaatgacgg cgtgcagggc aggaaaggca gaatgccgac tgatccacca aagggcgaag
      721 gctgttaccg ccttgctgcg ccgcgctgcg atcaggactg gcacgcgtat cacggcatcc
      781 ggcgcaaggt ttttcatttg ccccgtcccg atgagccagc cagccctgat tgccatgccc
      841 tgctgctgtt tctggaggac cggccgatcg gcgccattca ggtggatgac ttgcgcaatg
      901 aagctgccgc actgcggctt gtggccatcg acccagcctt tcagggcggc ggccatggcc
      961 gagcgatgct ggagcgggcg gagcagttcg tacgcggcct ggggtgcagc aaagcggtcg
     1021 tttatgcaac gccggaggcg gcgggctttt atcagaccgc tggctatgac gaggaagact
     1081 gggacgagat ctgcatgggc ggcatcgtgc aaatgctgaa gaagctggac tgacgaagcg
     1141 gcgaaacatg gacgaccgag ccggtgtcgc actgtcagcg cgtttaccgc ctcgttgcta
     1201 tcggacaaca ccgtcggctt cctgccatcc gacggcagga cagcaggaag ccgccgcgaa
     1261 actcggacgc ggtggcgagg ctggttatgc tcgcaagggc tccggcgggc catcgggcgg
     1321 aatcggtatc ggcgacggcg gttcctcgac cggcaccggt tcgtccggat ccggcaatac
     1381 cggcggatcg ccagggggaa ccggcggatc cgacagaacc agggttcggc gcgtcgtcaa
     1441 tggtgattcc attccggttg tttctgacac cataggtcct cacgtaggaa agccgcgtcg
     1501 gcgttcggga attcgccggg gatcgatgca ccggaccagg aaagccagcg gtttgaccct
     1561 cgttgcgtat gcagcataac ccgcggcctc tcagctccgt cattcacgga cgtgtcgttc
     1621 cggttcccgg cgacagggcc atttccgttt cgggaccggg ctcaggtgtc gccaccggaa
     1681 tcgg
//
LOCUS       DASWQN010000066         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_138548, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000066 DASWQN010000000
VERSION     DASWQN010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138548"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="VGC82_01115"
     CDS             <1..1276
                     /locus_tag="VGC82_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421905.1"
                     /translation="IYIQGYDPRGLAQYYRMFRTELRKFGRLYQLSATLGRPQGTPDG
                     EIASWTIETKADDWQTRTSYDFLRFEDFIQRDLAQPIWRTVLQAVWIYWRLVFAGTIA
                     RFWRANWRFATFITYPHFLLLLEAIGAAGLAFGFAKGLDALGIPDLFSIAAAIAVFVA
                     VLGTVLKYTENQTYLLYVLSDTIWTWEFSHRARPEWDQRIDRFAQHLVKTARESDAEE
                     IVVVGHSSGSFLGTEMLARALQLDPDLGRHGPRIVLLTIGGNFPIVGFHAVSQPFRDH
                     LRMLAVEPSIDWIDCQSRKDVMNFFPFDPIRGHDIDVGSNRRNPSIVSVRFRDIIRPE
                     HYNVFRWKFFRVHFQFVMANERPHAYDFFMIVCGPIPLTARVAAPDAALAIATGDPAA
                     RERAWKSIETAKTDASVAAELSGIEPSARRSG"
     gene            1637..>2445
                     /locus_tag="VGC82_01120"
     CDS             1637..>2445
                     /locus_tag="VGC82_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEY0421906.1"
                     /translation="MTSFSKTPLLDTIRTPDDLRKLKIEQVQQVADELRQETIDAVSV
                     TGGHFGAGLGVVELTTAIHYIFDTPRDRLIWDVGHQAYPHKILTGRRDRIRTLRTGGG
                     LSGFTKRTESDYDPFGAAHSSTSISAGLGMAVARDLSGGKNNVIAVIGDGAMSAGMAY
                     EAMNNAGAMNSRLIVILNDNDMSIAPPVGAMSAYLSRLYSGKTYRTLRDAAKQLGQHL
                     PKIIANRASRVEEYSRGFMMDGGTLFEELGFYYVGPIDGHNLDHLLPVLKNV"
BASE COUNT          450 a          815 c          719 g          461 t
ORIGIN      
        1 catctacatc cagggctatg acccgcgcgg gctggcgcaa tactaccgca tgttccggac
       61 cgaactgcgg aagttcggcc ggctgtatca gctgtccgcc accttgggcc gcccgcaggg
      121 cacacccgat ggcgaaatcg cgtcctggac catcgaaacc aaggcggacg actggcagac
      181 ccgcaccagt tacgacttcc tgcgctttga ggatttcatc cagcgcgacc ttgcccagcc
      241 gatctggcgt acggtgctgc aggcggtctg gatctactgg cggctggtgt tcgccggcac
      301 catcgcgcgg ttctggcgcg cgaactggcg gtttgcgacc ttcatcacct acccgcattt
      361 cctgctgctg ctggaagcga tcggcgcggc cggcctcgct tttggcttcg cgaaagggct
      421 ggacgcgctc ggcatccccg atttgttcag cattgcggcc gcgatcgcgg tgttcgtcgc
      481 cgtgctcggc accgtgctca aatacaccga aaaccagacc tacctgctct atgtgctgtc
      541 ggacacgatc tggacctggg aattctcgca ccgcgcccgc cccgaatggg atcagcgcat
      601 cgaccgcttt gcgcagcacc tggtcaagac cgcgcgcgag agcgacgccg aggaaatcgt
      661 cgtcgtcggc cacagttcgg gatcgtttct cggcaccgag atgctggcgc gcgcgctgca
      721 actcgacccc gatctcggac gccacggccc acgcatagtg ctgctgacga ttggcggcaa
      781 tttcccgatc gtcggtttcc acgccgtatc gcagccgttc cgcgaccatc tgcggatgct
      841 ggcggtcgag ccctcgatcg actggatcga ctgccagtcg cgcaaggacg tgatgaattt
      901 ctttccgttc gatccgatca ggggtcacga catcgacgtc ggttcgaaca gacgaaaccc
      961 cagcatcgtg tcggtgcggt ttcgcgacat catcaggccc gagcattaca acgtgttccg
     1021 ctggaagttc ttccgggtcc acttccagtt cgtgatggcc aacgaacggc cgcacgccta
     1081 cgattttttc atgatcgtct gcgggccaat ccccctcacc gcccgggtgg ccgcccccga
     1141 cgccgcgctg gcgatcgcca ccggcgatcc ggcggcccgc gaacgggcct ggaagagcat
     1201 agaaacggct aaaacggatg cctcagttgc cgccgaattg agcggcattg aaccttctgc
     1261 ccgccgcagc ggctaaaaag cggaggtatt tcagtccttg tcagcccttt ggcccggaac
     1321 cgggcctgcg ccggcgggtt ggctttggcg gcgggtttgg tgtacaaatt gcagctctat
     1381 gggctgagga gcgcggcctg ccgctgaatt ccagcggctg caagcacttc aaatagctaa
     1441 agaaactgac cgcgaagggt taaagctacc aacgtgatgc atatcacatg gttcgatccg
     1501 gagtgcatct aggctttcaa atcaggtacc ggccagccga gatggcaccc ggtgtctgga
     1561 ttttcacagc gcggttaacg cgcaccccat ctcgcgtcgg gccgttcgcc ctgacatgca
     1621 aaattggaat atcgctgtga cctcatttag taaaacaccg cttctggaca ccattcgtac
     1681 cccggacgac cttcgcaagc tcaagatcga gcaggtgcag caggtcgccg acgagctgcg
     1741 gcaggaaacc atcgacgcgg tctcggtcac gggcggccat ttcggcgccg gcctcggcgt
     1801 ggtcgaactg accaccgcga tccattacat cttcgatacc ccccgcgatc gcctgatctg
     1861 ggacgtcggc caccaagcgt acccgcacaa gatcctgacc ggacgccgcg accgcatccg
     1921 cacgctgcgc accggcggcg ggctatccgg cttcaccaag cgcaccgaga gcgactacga
     1981 tccgttcggc gcggcgcact cctcgacctc gatctccgcg ggtctcggca tggcggttgc
     2041 ccgcgacctc tccggcggca agaacaacgt gatcgccgtg atcggcgacg gcgcaatgtc
     2101 ggccggcatg gcctatgagg ccatgaacaa tgccggcgcg atgaactcgc gcctgatcgt
     2161 gatcctgaac gacaacgaca tgtcgatcgc gccgccggtc ggcgccatgt cggcctatct
     2221 gtcccgcctc tactccggca agacctatcg caccttgcgc gatgcggcca agcagctggg
     2281 ccagcatctg ccgaaaatca ttgccaaccg cgccagccgt gtcgaggaat attcccgcgg
     2341 cttcatgatg gacggcggca cgctgttcga ggaactcggc ttctactatg tcggcccgat
     2401 cgacggccac aacctcgacc atctgctgcc cgtcttgaag aacgt
//
LOCUS       DASWQN010000067         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1458530, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000067 DASWQN010000000
VERSION     DASWQN010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458530"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VGC82_01125"
     CDS             complement(<1..1458)
                     /locus_tag="VGC82_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEY0421907.1"
                     /translation="MTLRSKLTWASVAVLGAISFAIVALDRGEPVNAAWLVVASVCIY
                     FIAYRFYALFIANRVFGIDPARPTPAHRHNDGLDYVPTNRYVLFGHHFAAIAGAGPLV
                     GPVLAAQMGYLPGTLWILAGVVFAGAVQDMTVLFLSTRRDGRSLGDMIRTELGPVPGA
                     IAGIGVLLICIIILAVLALVVVKALMGSPWGTFAVFATIPIALLMGLYTRFIRPGRVG
                     EMSVIGAVLLVVALIGGRGVSETPELAAWFNLSGETLAVIIIGYGFVASVLPVWLLLA
                     PRDYLSTFLKIGTISLLAIGIVIVRPDLEMVAVTRFVDGSGPVWAGGVFPFLFITIAC
                     GAVSGWHALIASGTTPKMIENESQIRFIGYGGMLMESFVAIMALIAASVIHPGVYFAM
                     NSATGVIGTDPAHAAQVISSWGFTVTPDVLTGVAHEVGESTILSRTGGAPTLAVGMAS
                     ILSGFLGGPALAGLWYHFAILFEALFILTTVDAGTR"
BASE COUNT          352 a          522 c          524 g          252 t
ORIGIN      
        1 gcgggtgccg gcgtccaccg tcgtcaggat gaacagcgcc tcgaacagaa tggcgaaatg
       61 ataccagagg ccagccagcg ccgggccgcc gagaaacccg gacaggatgc tggccatgcc
      121 gacggccaat gtcggtgcgc cgccggtgcg tgagagaatg gtactttccc ccacctcatg
      181 tgcaacgccg gtgagcacgt caggcgtgac tgtaaacccc caggaagaga tgacctgggc
      241 cgcatgtgcc ggatccgtgc cgatcactcc ggtggcgctg ttcatggcaa agtagacgcc
      301 gggatgaatg acactcgcgg caatgagcgc catgatagcg acaaacgact ccatcaacat
      361 gccgccatac ccgatgaagc ggatctggct ttcgttctcg atcatcttcg gggtcgtgcc
      421 agatgcaatc agggcatgcc agccggacac tgccccgcag gcgatggtga tgaacaggaa
      481 tgggaatacg cctccggccc agactggccc actgccgtca acgaagcggg ttactgcgac
      541 catctccagg tcaggacgca cgatgacgat gccgatcgcc agcaagctga tcgtcccgat
      601 cttgaggaag gttgatagat agtcccgcgg tgccagcagg agccataccg gcagcaccga
      661 ggcgacgaag ccgtagccga tgatgatcac cgccagcgtc tcgccgctca gattgaacca
      721 ggcagcgagc tcgggcgtct cggatacacc gcgcccgccg atcagcgcta ccaccagcag
      781 cacggcgccg atcactgaca tctccccgac gcgtccaggc cggatgaatc gggtgtagag
      841 acccatgagc aaggcgatgg gaatggtggc gaacacagcg aaggtgcccc aggggctgcc
      901 catcagggcc ttgaccacga caagcgccag caccgcaagg atgatgatgc agatcagcag
      961 tacgccaatg ccggcaatgg ccccgggcac tggtccaagc tcggtgcgga tcatgtcgcc
     1021 gagcgaccgc ccgtcgcgcc tggtggagag gaacagcacg gtcatgtcct gcacggcgcc
     1081 ggcgaagacc acacccgcca agatccagag cgtgcctggc aagtagccca tctgggcggc
     1141 aagcaccggg ccgaccagcg gcccggcgcc ggcgatcgcg gcgaaatggt ggccgaacag
     1201 cacgtagcgg ttcgttggga cgtaatcgag gccatcgtta tggcgatgcg cgggtgttgg
     1261 ccgggcaggg tcgataccga acaccctgtt ggcaatgaaa agcgcgtaga accggtaggc
     1321 aatgaaatag atgcacacgg aggcaacaac cagccaggca gcgttgactg gctctcctcg
     1381 gtccaaggcg acgatggcaa aggaaatcgc gcccaagacc gccacactgg cccatgtcag
     1441 cttcgatcgc agcgtcatcg ttccgctccc tgcagcggtc tttggccgga ccaagccagc
     1501 cggccgctgg caactctcct acccagtagc cttgaacgac tgttgttctc cctagttgcg
     1561 tttgcatgac cggggcgccg tgcgggtcgg ggcttccgtc tgacgccggc ggaacgcggc
     1621 aaggcctcct atcgcctcga gtgcttgatc
//
LOCUS       DASWQN010000068         4942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1722494, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000068 DASWQN010000000
VERSION     DASWQN010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722494"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1685)
                     /locus_tag="VGC82_01130"
     CDS             complement(225..1685)
                     /locus_tag="VGC82_01130"
                     /inference="COORDINATES: protein motif:HMM:NF037725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HEY0421908.1"
                     /translation="MFDTIVPDRTAPRTIVVLPSLSIDQDVMSRIAGIHHYEERMLCM
                     LMLLRLPRTRLIYLTSQPIPETIIDYYLHLLQGIPARHARERLTMLACYDGSARSLTE
                     KILSRPRLLNRVREAIGDPGTAYITAFAVTSQERTLSVTLGIPVYGCDPALQALGSKS
                     GGRKLMREAGVAIPDGVEDLIDEDGIAAGLVELKSRDPGLHRAVVKLNEGFSGEGNAV
                     FRFDDAPPGEALMPWVRDRLPHLAFEARGMTWEAFLEKAREMGSVVEAFIEGDEKQSP
                     SAQFRIDPFGYVEPVSTHDQVLDGQVFLGCRFPADDAYRLAIQDEGAKVAALLGQRGV
                     LGRLAVDFVSVRRGDSWSNYGIEVNLRKGGTTHPFLMLQFLTGGAYDPVSGLFRTPAG
                     QLRFYYASDNLEAPHYKGLTPADLVDVAVLNGLHYDGTVQEGVVFHLIGALSEFGKLG
                     VVCVGGSPKRAEVLYRRTVEILDREQMPDYGGSVPD"
     gene            complement(1782..2705)
                     /locus_tag="VGC82_01135"
     CDS             complement(1782..2705)
                     /locus_tag="VGC82_01135"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0421909.1"
                     /translation="MASKQSQQLATLYKGWIAALAANPDMPLDELRSMFEHWGDVTAE
                     PGGVDYIETASAGIPALWAVPKSCAQDRVLLCAHGGGYVTGSMYSHRKVYGHFAKAIG
                     CRALIVHYGRAPENVHPGPVNDMAAAYGWLLDQGIEPKHIALTGDSAGGGLAITTLLR
                     VREQGLELPAASMPLSPWLDMDGAGASFDSNRERDVLVAREIIQGMSATFLGEEGDRR
                     HKLANPLYADLRGLPPVYIQVGGYETLLDDSHALAEALRLVDGEVKLDVYPEMQHVFH
                     FLAGVAPEADEAIGRLAAWVRPKLGLAQHPG"
     gene            complement(2720..4090)
                     /locus_tag="VGC82_01140"
     CDS             complement(2720..4090)
                     /locus_tag="VGC82_01140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0421910.1"
                     /translation="MFEIGVFHNGASSLPVVLTKDGVAVNDGSLAEVHAAAQEVLINQ
                     VRQGVLAEKLGFQSFWLTEHHFQPEGAEMSPNPLFTQMAIAVRTKKIRLGQCANIIVW
                     HHPVRIAEQIAQLDVISGGRVECGMGRGYQPRENETLGRPYGSTIQDQERNRKSFEEA
                     IAIIRKCWTEPSFVHRGENFSIPPSYTKWNHKQTIAYFGMDKVERRLEDVIAIGAPDM
                     YSAGNPVQATTTTLKELQVFPQPVQKPHPQIWEPLTSPRSIKWAAEHGVNGVFIVEPN
                     DRLKRNIDMYYEAAEKAGWPDMRDRGRFKYGWDAEKRRGIMTSRYIHITTPGKEKQAM
                     ERAARAMELQFDYYGPFGFGAVVARLDEPMFDLNKRVTAEMLREREIAIQGSKQFVID
                     KIMKMKKEVGYEDFCFLGWFELGGFEGREIEDQMQLFAEEVMPVIARECGGKVELPEC
                     GIDYGD"
     gene            4618..>4942
                     /locus_tag="VGC82_01145"
     CDS             4618..>4942
                     /locus_tag="VGC82_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY0421911.1"
                     /translation="MTERAVIGDSPKRREDARFVTGHGAYLDDLHFDQALHAVFLRSP
                     HAHARINAIDTADALHSPGVIAILTAKEVEADGLKPLRPTIEANVQTNEPFRFLPQPL
                     LAEDTV"
BASE COUNT          917 a         1641 c         1422 g          962 t
ORIGIN      
        1 caagcacggc catgatacat cggcgtcggg cggcgaaccg gccggcacct tttccggtca
       61 ccgtgagatt gctctagatt ggccaggcga acctgccggt cagaagccaa cgaagcctct
      121 tttccgtggc gatccgtgga gtcatctcgc ttctccgtga tgagacctgc gaacctggca
      181 aacccgccac cgccaaccaa aacgcgactc acgcccgccc tgtttcaatc cggcacagaa
      241 ccaccgtagt ccggcatctg ctcgcgatcg aggatttcca ccgtccggcg atacagcact
      301 tcggcccgtt taggcgatcc accaacacag acgacaccaa gtttaccaaa ctccgacagc
      361 gcgccgatca agtgaaacac caccccttcc tgcacggtgc cgtcgtagtg cagaccgttc
      421 agcaccgcga catccaccag atccgctggt gtcaaaccct tgtagtgcgg cgcttccaga
      481 ttgtccgacg catagtagaa acgcagttgt ccggccggcg tgcggaacaa gcccgagacc
      541 gggtcataag caccgccggt caggaattgc agcatcagga acgggtgcgt ggttccgcct
      601 ttgcgtagat tgacctcgat gccgtagttg ctccaactat cgccgcgccg caccgatacg
      661 aagtccaccg cgagccggcc cagcacacca cgctgcccca gcaaagccgc gactttcgcc
      721 ccttcatcct ggatcgcaag ccgataggca tcgtcggccg gaaagcggca gcccaggaag
      781 acctgcccgt ccaatacctg gtcatgagtg gacaccggtt cgacatagcc gaatggatcg
      841 atgcggaact gcgccgacgg cgactgtttc tcatcgcctt cgataaatgc ctcgaccacc
      901 gaacccattt cccgcgcctt ctccagaaag gcctcccagg tcatgccccg agcctcgaat
      961 gccagatgcg gcagtcggtc gcggacccag ggcatcaggg cctcgcccgg aggggcgtca
     1021 tcaaatcgga acaccgcgtt gccttcgcct gaaaatcctt cgttcagttt caccaccgct
     1081 cggtgcagcc ccggatcacg gctcttcagt tcgaccagcc ccgccgcaat gccgtcttca
     1141 tcgatcagat cctcgacgcc atccgggatc gccacaccgg cctcccgcat cagtttccgg
     1201 ccgccgcttt tggagcccag ggcctgcaac gcgggatcgc agccatacac aggaataccc
     1261 agggtgacag aaagcgttcg ctcctgcgac gttaccgcga aagcggtaat gtaggcggtt
     1321 cccggatcgc cgatcgcctc gcgcacgcgg ttgagaaggc gggggcggga caggatcttt
     1381 tccgtcagcg accgggcgct gccgtcgtaa caggcgagca tcgtcagtct ttcccgagcg
     1441 tgccgggcgg ggatgccctg cagcaagtgc aaatagtaat cgatgatcgt ttcgggaatt
     1501 ggctggctgg tcaggtagat cagccgggtg cgcggcaacc tcaacagcat cagcatacac
     1561 agcatccgct cctcgtaatg gtggatgccg gcgatcctgg acatcacgtc ctggtcgatg
     1621 ctcaggctcg gcagcaccac gatggttcgc ggggcggtgc ggtctggaac gattgtatcg
     1681 aacatgtccc gcaagcaggc ctgaagccga tcgaattcgg cctgctcctc gaccgagccg
     1741 ggtgtgattc gcctgccagg caagagcgtt ggctcctcaa gtcaaccggg gtgttgcgcc
     1801 agacccagct ttggccgaac ccaggctgcc agccgcccga ttgcctcgtc tgcctcgggt
     1861 gcgacaccgg ccagaaagtg gaatacgtgc tgcatctcgg gatacacgtc cagtttcacc
     1921 tcgccgtcta cgagccgtag cgcctcggcc aacgcatgac tgtcgtccag caaggtctcg
     1981 tatccgccga cctggatata gaccggcggc agaccgcgca ggtcagcata cagcggattg
     2041 gccagcttgt gacgtcgatc accctcctcc cccaggaacg tcgccgacat tccctggatg
     2101 atttcgcgcg caaccaggac atcgcgctcg cggttgctat cgaagctggc gccggcgccg
     2161 tccatgtcga gccacgggga caatggcatg cttgccgccg gcaattccag gccttgctcg
     2221 cgcacgcgaa gaagcgtggt gatcgccaag ccgccgccgg ccgaatcgcc ggtcagcgcg
     2281 atatgtttcg gctcaatccc ctgatccagc agccatccat aggcggcggc catatcgttc
     2341 accggccccg gatgcacatt ctccggcgcc cgcccgtaat gcacgatcag cgctcggcaa
     2401 ccgatcgcct tggcaaaatg tccgtacacc ttgcggtggg aatacatcga ccccgtaacg
     2461 tagcctccac catgcgcgca cagcagaact cgatcctgcg cgcagctctt cgggaccgcc
     2521 caaagcgctg gaatcccggc tgaagcggtc tcgatataat cgaccccgcc cggctccgcc
     2581 gttacgtcgc cccagtgctc gaacatcgac cgcaattcat ccagcggcat atcgggattt
     2641 gccgccagcg cggcgatcca gcccttatac agggtcgcga gttgttgact ttgtttgctg
     2701 gccattggcg cctccctttt cagtcaccgt aatcgatgcc gcattcaggc agctcaacct
     2761 tgccgccgca ctcccgggcg atcacaggca tgacctcctc ggcgaaaagc tgcatctgat
     2821 cctcgatctc gcggccttcg aagccgccaa gttcgaacca gccgaggaag cagaaatcct
     2881 cgtatcccac ttcctttttc atcttcatga tcttgtcgat gacgaactgc ttggatcctt
     2941 gtatggcgat ttcccgctcc cgcagcatct ccgccgtgac gcgtttgttc agatcgaaca
     3001 tcggctcatc caggcgggca acgaccgctc cgaagccgaa cgggccatag tagtcgaatt
     3061 gcagttccat cgcccgcgcg gcgcgttcca tcgcctgctt ctccttgccg ggcgtggtga
     3121 tgtggatgta gcggctggtc atgatgccgc ggcgcttttc ggcgtcccag ccgtatttga
     3181 acctgccgcg gtcgcgcata tcgggccatc cggccttttc agcggcctcg tagtacatgt
     3241 cgatgtttct cttcaaccgg tcgttgggct cgacgatgaa cacgccattt acgccgtgtt
     3301 cggccgccca cttgatggag cgcgggctgg tcagcggctc ccagatctgc ggatgcggtt
     3361 tttgcacagg ctgtgggaag acctgcagtt ccttcagggt cgtcgtcgtc gcctgcaccg
     3421 gattgccggc ggagtacatg tccggggcgc cgatcgcgat cacgtcctcc agccgccgtt
     3481 ccaccttgtc catgccgaaa taggcaatgg tctgcttgtg gttccacttg gtgtatgagg
     3541 gcgggatgga aaagttctcg ccgcgatgca cgaacgacgg ttcggtccag catttgcgga
     3601 tgatggcgat cgcttcctcg aacgatttgc ggttccgctc ctggtcctgg atcgttgacc
     3661 cataggggcg gcccagcgtc tcgttctcgc gcggctgata cccgcgcccc atgccgcatt
     3721 cgacccgacc tccgctgatc acatcaagct gcgcgatctg ttccgcaatc cggaccggat
     3781 gatgccagac gatgatgttg gcgcactggc ccaggcggat tttcttcgtc cgaacggcga
     3841 tggccatttg ggtgaacagc gggttcggac tcatttccgc gccttcgggc tgaaaatgat
     3901 gttcggtgag ccagaacgac tggaagccaa gtttctcagc aaggacgccc tggcgaacct
     3961 gattgatcag gacctcctgc gcggcggcgt gcacctcggc gagcgaacca tcgttcaccg
     4021 ctacgccatc tttcgtgagg accacgggaa gactggacgc gccattgtgg aagacgccaa
     4081 tctcgaacat cgcactccct ccttatgatt tctggccctt tgactcttgg tccttggact
     4141 ttcggttgtc gctgacgctg ccggtcgaac cgtcgcggcg tcagtaaccg cctggtgtct
     4201 gcggaccgca agcggcctgg aggttgggac gccgatcaat cacccaatcg tgtgtttggc
     4261 gacggcattg ccctgggcgc ttagctgaca agtggcagca gcgctgcgta ggcgcctatc
     4321 agcccgatga tggttaccac gatctcgaca tagacgcgca tgggaagcct ccgtgtgcct
     4381 gtggcagcaa acatgccggt tcgcgtcgag gcggcgctgt ggcctgcctc acacatgcaa
     4441 actggtcagc gtccgtggcc gatgattgga gatgtgaagg taagattgtg gcagtgccgc
     4501 cgggcccggg cggccatgga tcgcggcgat gccatgcgaa tgtcagctga agccttgccc
     4561 gcctccccgg gctcggatca gactgttgaa ccccggaccc tcaaggaact gccacccatg
     4621 accgaacgcg cagtgatcgg cgactccccg aaacggcggg aggatgcccg tttcgtcacg
     4681 gggcacggcg cctatctgga cgatctgcat ttcgatcaag ccctccatgc cgtcttcctg
     4741 cggtcgccgc acgcccatgc ccgcatcaac gccatcgaca cggccgatgc gcttcattcg
     4801 ccaggcgtca tcgcgatcct cacggcaaag gaggtcgagg ctgacggcct gaagccgctg
     4861 cgtccgacca tcgaggccaa cgtccagacc aacgaaccgt tccgcttcct gccgcaaccg
     4921 ctgctggccg aggataccgt cc
//
LOCUS       DASWQN010000069         3348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1458675, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000069 DASWQN010000000
VERSION     DASWQN010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458675"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            572..1768
                     /gene="pimC"
                     /locus_tag="VGC82_01150"
     CDS             572..1768
                     /gene="pimC"
                     /locus_tag="VGC82_01150"
                     /EC_number="1.3.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018515 - pimeloyl-CoA dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-CoA dehydrogenase large subunit"
                     /protein_id="HEY0421912.1"
                     /translation="MDLNFTQEEIAFRNEARHFFRTAVPESIRAKVAEGEGLTRDDMI
                     NAQRILNARGWATVNWPVEWGGQDWTPIQVYLYQDEMQQANVPPPIAFNVSMVGPVIA
                     QFASQEIKQRFLPATANVDIFWCQGFSEPGSGSDLASLRTKAEKKGDKYIVNGQKTWT
                     TLAQYADWIFCLVRTDPSAKSQEGISFLLIDMKTPGITVRPIVTIDGGREVNEVFFDD
                     VEVPVENLVGQENKGWDYAKFLLGNERTGIARVGASKAKIARIKELAALEFVGERALI
                     ESPRFQEKLAAIEVELKALEMTQLRVVSDERKMAKGRQNPASSILKLKGSVIQQQLTE
                     LLMEVVGPYVMPYQRDFDGNNEPVIGPEYASEAAPMYFNYRKVSIYGGSNEIQRNIIA
                     KAILGL"
     gene            1806..2954
                     /locus_tag="VGC82_01155"
     CDS             1806..2954
                     /locus_tag="VGC82_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0421913.1"
                     /translation="MDFDLSEDQRLLKDSVDRLVADQYQFEQRKKYAREPTGYSAAMW
                     DQITEMGLLGLPFAETLGGFGGGAVETAIVMDAFGRGLVLEPFFATVILGGGLLRRAA
                     SDSVLGALAPKVAAGKMKLAFAHVERHSRYDLADVGTTARKDGSGYILDGAKSVVIHG
                     DSADRVFVTARISGDRRRRDGIGLFLVDPATQGFSRRGYPTQDGQRAAEITLSGARVG
                     VEDLISDNALPMIEHVVDEAIAALCAEAVGAMQSMQDLTLEYLKTRKQFGRPIGSFQV
                     LQHRSVDMLVAVEQARSMAMFAAVMAAEENPIERRRAISAAKVQIGRSGRHVGQEAIQ
                     LHGGIGMTNEYAVGHYFKRVTMIDQLYGDADTHLAYLAANGGLFGKVA"
     gene            2951..>3348
                     /locus_tag="VGC82_01160"
     CDS             2951..>3348
                     /locus_tag="VGC82_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY0421914.1"
                     /translation="MKQAVLLDLDGTLSNSRPGIEACFRHMLAELGHDPAVAGDLTWA
                     VGPPIAVSIGRLLAKYSDDRVDLGVTTYRACYTASGLYQCAVYPGVPEMLAALTEAGH
                     ALCVATSKRSDYAERVVDYLGLRQYLPQVYG"
BASE COUNT          659 a         1007 c         1052 g          630 t
ORIGIN      
        1 gcggtaagtc agtgtccatc ggcgtgcggg agcccgtgga cggcgcgcaa tggctgcaca
       61 atctgtgtag tccaggcaag ccatcgatgt gtggctcagc gacgtgggcc tgccgggtgg
      121 cacgaatgga tggcagcttg ccgatgcggc aggggttttc ctcccccggt ccaaggtcct
      181 gttcgtcacc ggctatgccg gaaccgccgg ctcggcgatg gcgtgctgca gtcgaacatg
      241 cgggtgatca cgaagccttt tgcgctggaa atgctggcta ccaaatctat gctactctct
      301 gaaacgaccc ggtcgtgcca atggtgccct gcggatgttc acatccgctc ccatttcttc
      361 ggaaaattcg attcacgcca gccagcgttg ggttagacca tggggtaggt tggcatggca
      421 gcggcgtgaa aatgacacca taattggcga ccggccggcg ccgaagcgtc aacgtttccg
      481 cttgtccatc tacgtcagcg cgggtcgcac cgggtttgac gccagccagc cggccctata
      541 tcgagccagt caagcaaagg gagccgcctc catggacctg aatttcaccc aagaggaaat
      601 cgccttccgc aacgaagcgc gccatttttt ccgcaccgcc gtgccggagt ccattcgagc
      661 caaggtcgcg gaaggcgagg gcctgacgcg cgacgacatg atcaacgcgc agcgcatcct
      721 gaacgcgcgc ggctgggcca cggtgaactg gccggtcgaa tggggtggcc aggactggac
      781 gcctattcag gtctatctgt accaggacga gatgcagcag gccaacgtgc cgccgccgat
      841 cgccttcaac gtgtcgatgg tcggtccggt catcgcccag ttcgccagcc aggagatcaa
      901 gcagcggttc ctgcccgcca ccgccaacgt cgatatcttc tggtgccagg gcttttctga
      961 gcccggttcc gggtccgacc tcgcgtcgct gcgcaccaag gcggagaaga agggcgacaa
     1021 gtatatcgtc aacggccaga agacctggac gactttggcg cagtatgccg attggatatt
     1081 ctgcttggtg cgcaccgacc cgtcggcgaa gtcgcaggaa ggtatttcct tcctgctgat
     1141 cgacatgaaa accccgggca tcacagtccg cccgatcgtc accatcgacg gcggccgcga
     1201 agtgaacgaa gtattctttg acgacgtcga ggtcccggtc gaaaaccttg tcggccagga
     1261 aaacaaaggc tgggactatg cgaagttcct gctgggcaat gaacgcactg gcatcgcccg
     1321 cgtcggcgca tcgaaggcca agatcgcccg catcaaggag ctcgccgcgc tggagttcgt
     1381 gggcgagcgg gcgttgatcg aaagcccccg ctttcaggaa aagctggctg cgatcgaagt
     1441 ggagttgaag gcgctggaaa tgactcagct tcgggtggtt tcagatgagc gcaagatggc
     1501 caaggggcgg caaaatccgg cgtcctcgat ccttaagctg aaaggatcgg ttatccagca
     1561 gcaactaacc gaactgctga tggaggtcgt cggaccctac gtcatgccct atcagcgtga
     1621 cttcgacggc aacaacgaac ccgtcattgg tcccgaatac gcttcagagg ccgcgccgat
     1681 gtacttcaac tatcggaagg tctcgatcta cggcggatcg aacgaaatcc agcgcaacat
     1741 catcgccaag gcaatcctgg gcctttgaga tgcggcacgg cgactgaacc aagaaggtca
     1801 agacaatgga tttcgatctc tccgaagacc agcgcctgct gaaagacagc gtggatcgcc
     1861 tggtcgccga ccagtatcaa ttcgaacaac gtaagaaata tgcccgcgaa cccaccggct
     1921 acagtgctgc catgtgggac cagatcaccg agatgggcct gctcggtttg ccgttcgcgg
     1981 aaacacttgg cggctttggc ggcggtgcgg tggaaaccgc catcgtcatg gacgcgttcg
     2041 gccgcggtct ggtgctggaa cctttctttg ccacggtgat cctcggcggc ggcttgctgc
     2101 ggcgtgcggc atcggactcc gtcctgggtg ccctggcgcc gaaggtagcc gcgggcaaaa
     2161 tgaaactggc gtttgcccat gtcgaacggc attcccgcta cgatctcgcc gacgtcggca
     2221 ccaccgcgcg taaggacggc agcggataca tcctggacgg cgccaaaagt gtcgtaatcc
     2281 atggcgatag cgccgaccgc gtcttcgtca ccgcccgtat ctccggcgac cgccgccgcc
     2341 gtgacggcat tgggctgttc ctggtcgatc ccgcgacaca aggcttcagc cgacgaggct
     2401 atccgacgca ggatggccag cgcgccgccg agatcacgtt gagcggtgcc cgcgtcggcg
     2461 tggaagacct gatcagcgac aacgccttgc cgatgatcga gcacgtggtc gatgaagcca
     2521 ttgccgccct ctgcgccgag gccgtcgggg caatgcagtc gatgcaggac ctgacactgg
     2581 agtatttgaa gacccgcaag cagtttggcc gcccgatcgg gtcgttccag gtgttgcagc
     2641 accggtctgt ggacatgctg gtggcggtgg aacaggcccg cagcatggcg atgttcgccg
     2701 ccgtgatggc cgctgaggaa aaccccatcg agcgtcgccg agcgatctcc gctgccaagg
     2761 tgcagatcgg ccgctcgggg cgccatgttg ggcaggaggc gattcagctg catggcggca
     2821 tcggcatgac caacgaatac gctgtcggac actacttcaa gcgcgtgacg atgatcgacc
     2881 agctttacgg cgatgccgac acgcatctgg cgtatctggc cgcgaatggc ggcctgttcg
     2941 gcaaagtcgc gtgaaacagg cagtattgct cgacctggac gggactcttt cgaattcccg
     3001 ccccggaatt gaggcctgct ttcgtcacat gctggctgaa ctgggtcacg atccggcggt
     3061 cgcgggcgac ctgacatggg cggtgggacc gccgatcgcc gtgtcgatcg gtaggctgct
     3121 ggcgaaatat agtgacgacc gggtggacct tggcgttacc acgtaccgtg cctgctatac
     3181 cgcctctggt ctctaccaat gcgctgtcta tcctggtgtg ccagagatgc tggcggcctt
     3241 gaccgaggcc ggccacgccc tgtgcgttgc tacatccaaa cgcagcgact atgccgaacg
     3301 ggtcgtcgac tatctcggtt tgcggcaata cttgccacaa gtttacgg
//
LOCUS       DASWQN010000070         8006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1326938, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000070 DASWQN010000000
VERSION     DASWQN010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326938"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..940
                     /locus_tag="VGC82_01165"
     CDS             158..940
                     /locus_tag="VGC82_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0421915.1"
                     /translation="MNWRIVLALLALLAPFHVSADIGYAPGQATSQLCRQAIAAAERA
                     HGIPAHLLAAIARVESGRRDQTSGAFNPWPWTTNVDGVGAFYETKAQAVAASVSIRPK
                     VVRSIDVGCMQISLTYHPDAFSSMDQAFDPASNAEYGARFLSQLFEKTASWPRAVELY
                     HSATPDLGRDYQLKVYAGLPEEQRIADVAQPPALANAWAATIHHPLPSIATGSHPMPS
                     MSSRQSPPHIIALASSSPNGSTPGRSLDSYRSMPVRIAFRGP"
     gene            complement(973..2163)
                     /gene="metC"
                     /locus_tag="VGC82_01170"
     CDS             complement(973..2163)
                     /gene="metC"
                     /locus_tag="VGC82_01170"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEY0421916.1"
                     /translation="MTDDIKKPGFRTTLSHVGRATKREHGFVNPPLLRGSTVLVPTVK
                     ERQAMQAKRGERVLAYGTGGSATHWALEDAIAAIEGGTRCYIVSTGLAAVTTPLLAWL
                     KAGDHMLMPDHVYGPARNFASGMLRGYGVETTFYTPEIDEAGLSALMRPNTKVVYTES
                     PGSHTFEVQDVPAIARAAHAGGAKVFMDNTWGIHHFQPFTHGVDVSIQAATKYLAGHS
                     DVLIGSITVNNEADWQILLAAGRTLGQYASPDDCWLTLRGIRTLAVRLQTQMESGMQV
                     AHWLRQQPEVAQVLHPGLPGAPGHDLWRRDFSGACSLFGIEFKPSFSAQDTHRFVEAL
                     DLFGIGASWGGYESLAVPTTGFVTRTAGSGTFAGPVVRMHIGLEDADDLIADLRHGLV
                     ALRS"
     gene            complement(2166..3005)
                     /gene="sseA"
                     /locus_tag="VGC82_01175"
     CDS             complement(2166..3005)
                     /gene="sseA"
                     /locus_tag="VGC82_01175"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HEY0421917.1"
                     /translation="MGPLVTTAWLAGELGKPDLVVFDATKYLPNEPKDGAAEFIRVHI
                     PGARYFDIDQIADPDTDLPHMVPTQGRFAKLMAALGVANTSRIVFYDQKGLASAARGW
                     WLMGLFGHDDAAVLDGGLPKWLKEGRPVQEGEPEVPRRSEFRPDYRASRVRGVGDMLR
                     NVLTRSEQVLDARAAGRFTGAIPEPRAGMRSGHIPGSISLPYTDLLQEDGTFRPAAEV
                     RRRFEAAGVESGRPLVTSCGSGVTACILTLGLRVAGLPEGAVYDGSWTEWGGRSDTPI
                     EVG"
     gene            complement(3098..4432)
                     /locus_tag="VGC82_01180"
     CDS             complement(3098..4432)
                     /locus_tag="VGC82_01180"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0421918.1"
                     /translation="MDLPYRTKPQPQVNQLIASLHPRHLGAELTQGLASARVVNIVGP
                     RQAGKTTLVRDLFKGGRFVTLDDENVLAALDDDPVGQLDALVAAAGDLPLVIDEAQRS
                     KRLALSIKRIVDERRRMGQFVLTGSSNIFTTAQVADSLAGRVQTLTMLPLSAAEIHHA
                     GPALLFDWAQQDGVALASLPDPAIWTRNEYIDLLLRGGYPEIRLLHNRARQRRYHDYV
                     NTIVDRDVADILRIRKTDSMRRLIEQLAARTSYEVNIQELGGIIGIQRPTMDAYLDVL
                     AKLSLVTRLPAWTSGETGRDIRQPKVHLVDTGIVSALRGMGPDTFLADANPSALGGLL
                     ETFVYVEILKNLPYQREHWRLYHWRSSRGREIDILAEAGRTLIGFEVKASATVTSEDF
                     RHLRWFAEDGPGFGWNVFGIVIYLGNRPLSFGPRLIALPLSTFWAYPQQPEA"
     gene            4636..5352
                     /locus_tag="VGC82_01185"
     CDS             4636..5352
                     /locus_tag="VGC82_01185"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0421919.1"
                     /translation="MIVADVMTRNVISVAPDATVEEAAKLMLSRYVSGLFVVDKAGDL
                     VGVVTEGDLLHREEIGTQRHRPWWLRLLASPAKQAADFTHASGRYVRDVMTKDVLSIA
                     KSGSLDDVVTTMEKYRIKRLPVTDGGRVIGVVSRADLLRALIGRVQNPEASVTDDLSI
                     RAAILNALEAQPWAPITTLNVTVTDGVVDVWGTITNEQERRAIHVVAENTAGAKSVRD
                     HLACIEPYSGIVMSGPDETP"
     gene            5354..6883
                     /gene="glpD"
                     /locus_tag="VGC82_01190"
     CDS             5354..6883
                     /gene="glpD"
                     /locus_tag="VGC82_01190"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEY0421920.1"
                     /translation="MSIDFDLAVVGGGINGTGIARDAAGRGLSVLLLEQGDLAGATSS
                     ASTKLIHGGLRYLEYHEFRMVREALAEREVLLRAAPHVIWPLRFVLPHHAGLRPWWMV
                     RAGLFLYDHLGTLWGSGRLLRKTRVVDLRTDPTGVPLKPEFTAGFEYSDCWVDDARLV
                     VLNARAAAELGADIRTRTACLSARRIDGIWSLALGSGEEVRARVLVNAAGPWVSHFLS
                     GALGYHASTRVRMVKGSHIVVRRLFQHDRCYIFQNRDGRVCFAIPYERDFTLIGTTDS
                     DFEGDPYAASVSPVEEAYLLSAVSSYLRVPVDPASIVWRYAGVRPLRDDGSDKAQEAT
                     RDYVLELDGAPPVLSVFGGKITTYRRLAEAAMAKLAPFFPNMRKPWTANARLPGGDFP
                     WDGATALRDSLQRRYPFLAPTTIERFVRSYGTLTIEMLGDAASAAALGTVVGADMTER
                     EIVWLMRTEWARSADDILWRRSKLGLRFDGTQRAKLEALVAKAGSSAAHSPGASIPRT
                     A"
     gene            6967..7164
                     /locus_tag="VGC82_01195"
     CDS             6967..7164
                     /locus_tag="VGC82_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421921.1"
                     /translation="MSASDSRYTAQNLMTVHGLRAQAVVHERIAEARQQGDTSGLERW
                     QNVEAAISELRKTAPRDASKG"
     gene            complement(7166..7240)
                     /locus_tag="VGC82_01200"
     tRNA            complement(7166..7240)
                     /locus_tag="VGC82_01200"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7206..7208),aa:Gln,seq:ttg)
     gene            complement(7290..>8006)
                     /locus_tag="VGC82_01205"
     CDS             complement(7290..>8006)
                     /locus_tag="VGC82_01205"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY0421922.1"
                     /translation="ALSLSVTGPFATALDADADNLVLRAARALAARAGCPALARLVLE
                     KNLPVASGIGGGSADAAAALRLLCRLWRIEPSVEDLAQLACDLGADVKVCLVARPALM
                     SGVGEVLVPAPSLPVAGLVLVNPGVAVSTPSVFRARSGEFSGRAGFPAHGWRDVRALV
                     ADLAATRNDLERPARMLAPAIGTALDALAATPGCRLARMSGSGATCFGLFDTPEAARR
                     ATGGIVRDGWWVWGGGMNDQ"
BASE COUNT         1552 a         2562 c         2442 g         1450 t
ORIGIN      
        1 ccaatccagg tctcgatgac gagtaagtat cgtcaggatt cgagacgcgt ccaatgctga
       61 gaatatgatg gcaaggctct caagtacagt cgcatgtatc ttgtgtcgta acgtccgcat
      121 caaccttttg ttcatatttg accaataaac caagggtatg aattggcgta tcgtccttgc
      181 ccttctggcc cttctggctc cattccatgt ctctgcggac atcgggtacg cgccagggca
      241 ggcaaccagc cagttgtgcc ggcaagcaat cgcggcggcc gaacgggcgc atggcatccc
      301 ggcccatctg ctggcagcga tcgcacgcgt cgaaagcgga cgcagggacc agacgtcagg
      361 cgcgttcaat ccctggccct ggacgactaa cgtcgatggc gtgggcgcct tttatgaaac
      421 caaggcgcag gccgtggcgg catcggtttc aatacgtccc aaggtcgtgc ggtcgatcga
      481 cgtcggctgt atgcagatca gcctgacgta tcacccggat gcattctcat cgatggatca
      541 ggcattcgat ccggcctcca atgccgaata cggagcccgc tttctgtcgc agcttttcga
      601 aaaaaccgct tcctggccgc gggcggtgga actgtaccat tccgctacgc cggacctggg
      661 ccgggattat cagctcaagg tttatgctgg tttgccggaa gaacagagga tcgcggacgt
      721 cgcacaaccc cctgcactgg ccaacgcatg ggcggctacc atccaccatc cgctgccatc
      781 gatcgccacg ggcagccacc caatgccatc aatgtcttcg cggcagagcc caccgcatat
      841 cattgcgctg gcatcaagtt ctccgaatgg atcgacgccc ggccgatcgc tcgactccta
      901 tcgatcgatg ccggtacgaa tcgcgttccg ggggccatag aacactgcct ggccttcgac
      961 agccagccgc gctcaggagc gtagcgccac gagaccgtgc ctcaggtcgg cgatcagatc
     1021 gtcagcgtcc tccagcccga tatgcatccg caccaccggt ccggcgaacg tgccgctgcc
     1081 cgccgtgcgg gtgacaaacc cggtggtcgg tacggcaagg ctttcatagc cgccccagga
     1141 tgccccaatc ccgaacagat ccagcgcctc cacgaagcga tgcgtatcct gtgctgagaa
     1201 gctcggcttg aactcgatcc cgaacagact gcaggcgccg gagaaatcgc gccgccacaa
     1261 atcatgcccg ggcgcaccgg gcagacccgg gtgcaggacc tgggcgacct ccggttgctg
     1321 gcgcagccaa tgcgcgactt gcattccaga ttccatctgg gtctgcagcc gcaccgccaa
     1381 cgtacgaatc cctcgcagcg tcagccagca atcgtcggga ctggcatact gccccagcgt
     1441 ccggccggcc gcgagcagga tctgccaatc cgcttcgttg ttcaccgtaa tggagccgat
     1501 caatacatcc gaatgcccgg ccagatactt tgtcgccgcc tggatcgaga catcgacacc
     1561 atgtgtgaac ggctggaagt ggtggatgcc ccaggtgttg tccatgaaca cttttgcccc
     1621 tccggcatgg gcggcacggg cgatcgccgg cacgtcctgg acctcaaacg tatggctgcc
     1681 cgggctttcg gtataaacca ccttggtgtt cggccgcatc agcgcgctta aaccggcctc
     1741 gtcgatctct ggtgtataga aggtcgtctc cacgccgtaa ccgcgcagca tgccagaggc
     1801 aaaatttcgc gccggaccgt acacatgatc cggcatcaac atgtggtccc cggccttcag
     1861 ccatgccagc agcggcgtcg tcaccgctgc cagaccggtg gagacgatat agcagcgggt
     1921 gccaccctcg atcgcggcga tggcatcctc gagcgcccaa tgagtcgcgg acccgccggt
     1981 gccatacgcc agcacccgct cgccccgttt ggcctgcatc gcctgacgtt ccttgacggt
     2041 aggaaccagc acggtcgagc cacgcagcag cggcggattg acgaacccat gctcccgctt
     2101 cgtcgctcgg ccaacatgcg aaagggtcgt gcggaagccg ggtttcttga tatcgtcagt
     2161 catgctcaac ccacctcgat cggggtatcg gagcgaccgc cccattccgt ccatgatccg
     2221 tcatacaccg cgccctcggg aaggccggcg acgcgcagtc ccaatgtcag gatgcaggcg
     2281 gtgacgcccg agccgcagct tgtgaccaga gggcgtccgc tttccacgcc ggcggcctcg
     2341 aagcggcgcc ggacttccgc agccggccgg aacgtgccgt cttcctgcag cagatcggta
     2401 taaggcaggc tgatcgagcc gggaatgtgg ccgctgcgca tgccggcgcg tggctccgga
     2461 atcgcgccgg tgaagcggcc ggcagcccgg gcatccagca cctgctccga ccgggtgagc
     2521 acgttgcgca gcatgtcgcc gacgccacga acccggctgg cgcgatagtc ggggcggaat
     2581 tccgaccgcc ggggcacctc gggttcaccc tcctgcaccg gtcggccttc cttcagccat
     2641 ttcggcaggc caccatccag caccgccgcg tcgtcatggc cgaacaggcc catcaaccac
     2701 cagccgcggg cagccgaggc cagacccttc tgatcgtaga acacaatccg ggaggtgttg
     2761 gcgaccccaa gcgccgccat cagtttcgcg aagcggcctt gggtcgggac catatgcggc
     2821 aagtcggtat cagggtccgc gatctgatcg atgtcgaaat atcgagcgcc aggaatatgg
     2881 acgcggatga attcggctgc gccatctttc ggctcgtttg gcaggtattt cgttgcatcg
     2941 aacaccacca agtccggctt gcccagttcc ccggccagcc acgcagttgt aacgagagga
     3001 cccatgttta ttcccattgg cgaaagccgc catcctaaag gcagtcgcga ccagatcaaa
     3061 ggtgacgcga ccgacatcag tgggccacat cgcgacatca tgcctctggt tgctgtggat
     3121 aggcccagaa cgtcgaaagc ggcagggcga tcaaacgcgg tccaaagctg agcgggcggt
     3181 tgccgagata gatcacgatg ccgaagacgt tccagccaaa cccaggcccg tcttccgcaa
     3241 accagcgaag atgccggaaa tcctcggagg ttacagtcgc cgaggccttg acctcaaagc
     3301 cgatcaaggt gcggccggcc tcggccagga tatcgatctc ccgtcctctg gagctccgcc
     3361 aatgatacag gcgccaatgc tcgcgttgat aaggcaggtt cttcaggatt tcgacataca
     3421 caaaagtctc cagcagtcct ccaagcgcgc tgggatttgc gtcagcgagg aaagtgtccg
     3481 gacccatgcc gcgtaaagcc gacacgattc cagtgtcgac gagatgaacc tttggctggc
     3541 ggatatcgcg tccggtctcc ccggatgtcc atgccggaag ccgcgtcacc agcgatagct
     3601 tggccagaac gtcgaggtag gcgtccatcg ttggccgttg gataccgatg atcccgccca
     3661 gttcctgaat attgacctcg taggatgtgc gggcggcaag ctgctcgatc aggcgccgca
     3721 tcgaatcggt cttccggatc cgcagaatat ccgccacgtc gcggtccaca atcgtgttca
     3781 cataatcgtg gtagcgccgc tgccgcgctc ggttgtgcaa aaggcggatc tccggatatc
     3841 cgcctcgcag caacaggtcg atgtactcgt ttcgcgtcca tatggccggg tcaggcagcg
     3901 aggccaacgc aacgccgtct tgctgtgccc aatcgaacag gagtgccggc cccgcgtgat
     3961 gaatttcagc agcgctcaaa ggcagcatcg tcagcgtctg cacccgcccc gcaagagagt
     4021 cggccacctg agcggtggtg aaaatgttgg atgatccggt caagacgaac tggcccatcc
     4081 gccgccgctc atcgacgatt cgcttgatgg aaagtgccaa gcgtttagag cgctgcgcct
     4141 catcgatgac aagcggtaag tccccggccg ccgcgaccag cgcatcgagt tgccccacgg
     4201 gatcgtcgtc caaggccgcc agcacgtttt cgtcgtcaag tgtgacgaac cgcccgccct
     4261 tgaacaaatc cctaaccaaa gtggtcttac cggcctgccg gggccccacg atgttgacta
     4321 cccgagcgga tgccagaccc tgcgtgagtt ccgccccaag atggcgcgga tgaagggatg
     4381 cgatcagttg atttacctgt ggttggggct tggttcgata gggtaaatcg ataccagaaa
     4441 cctggcagaa tgtatattag aaagaaggta aaacatacaa cagagacagg gtggaattca
     4501 ccttacctgg tttttcggga taattgaatc ttgacctggc ctcgcatcgg cgtcgcgcgc
     4561 aatgctcgac acgggatggc ttaccggctt gctgcacagg tgtatcctac tccgatacac
     4621 cggagggggc gcacaatgat cgttgccgac gttatgaccc gcaacgtcat ctcggttgca
     4681 ccggacgcga ccgtggagga agcggccaag ctgatgctgt cccggtacgt ttccggactg
     4741 ttcgtcgtgg acaaggccgg cgacctcgtc ggcgtcgtga cggaaggcga ccttctgcac
     4801 cgcgaagaaa ttggcactca gcgccaccgt ccgtggtggc tgcgcctact tgcctctcca
     4861 gccaaacagg cggcggattt tactcatgcc tctggccgtt acgtccgtga cgtgatgacc
     4921 aaagacgtgc tcagcatcgc caagagcggg tcgcttgacg atgttgtcac gacgatggaa
     4981 aaataccgta tcaagcgcct gcccgtgact gacggcggcc gtgtcatcgg tgtggtcagc
     5041 cgagctgatt tgttgcgggc gttgatcggg cgggtgcaga atcccgaagc atccgtaacc
     5101 gatgatctga gtattcgtgc cgcgatcctg aatgcactgg aggctcagcc ttgggcgccg
     5161 atcaccacgt tgaacgtcac cgtcaccgat ggcgtcgtcg atgtctgggg aacgatcacc
     5221 aacgaacagg agcgccgcgc cattcatgtt gtcgcggaaa acaccgctgg ggccaaatcc
     5281 gtccgtgacc atctggcgtg tatcgagccc tattccggca ttgtgatgtc aggaccggac
     5341 gaaacgccat agcgtgagca tcgactttga cctcgccgtc gttggtggcg gcatcaatgg
     5401 cacgggcatt gcccgcgatg ccgctggacg ggggctgtcg gtgctgctgc tggaacaggg
     5461 cgacctcgcg ggcgctacgt cctcagcctc gaccaagctg attcatggcg gcctgcgcta
     5521 cctggaatac catgaattcc gcatggtgcg cgaagccttg gcagagcggg aggtgctgct
     5581 gcgcgccgcc ccgcacgtca tctggccgct tcgtttcgtg ttgccgcacc atgctgggtt
     5641 acgaccatgg tggatggtcc gggcgggttt gttcctctac gaccatctcg gtaccctttg
     5701 gggtagcggt cgcctgctgc ggaaaacccg agtggtggac ctacgaacgg accctaccgg
     5761 cgtgccgcta aagccggagt tcaccgccgg cttcgagtat tccgactgct gggtggatga
     5821 cgctcgtctg gtggtgctga atgcccgcgc cgccgccgaa ctgggcgccg acatccgcac
     5881 ccgcaccgcc tgcctttccg cccggcgtat cgatggcatc tggtccctgg cacttggctc
     5941 tggtgaggaa gtccgggccc gggttctggt caacgccgcc ggaccttggg tatcccactt
     6001 tctgtcgggc gcactcgggt atcatgcatc aacccgggtg cgcatggtga aaggcagcca
     6061 catcgtcgtc cgccgtctgt tccagcatga ccgctgctac attttccaga accgcgacgg
     6121 ccgcgtctgc tttgccattc cctacgagcg ggatttcacc ctgatcggca ccaccgactc
     6181 ggacttcgaa ggcgatccgt acgcggcctc cgtttccccc gtcgaggaag cctatctgct
     6241 ttccgccgtc agcagttacc tgcgcgtccc ggtcgatccg gccagcatcg tgtggcgtta
     6301 cgccggggtg cgtccgctac gcgatgatgg atcggacaag gcgcaggaag ccacgcggga
     6361 ttatgtgctg gaactggacg gcgcgccgcc ggtgctgagc gtgttcggcg gcaagatcac
     6421 gacataccgc cggctcgccg aggcggccat ggccaagctg gcaccattct ttcccaacat
     6481 gaggaagcct tggaccgcga acgcccggtt gcccggaggc gattttccat gggacggggc
     6541 gactgcgttg cgggactcgc tgcagcggcg gtatccgttc ctggcgccga cgactatcga
     6601 acggttcgtg cgcagctacg gcacgctgac gatcgagatg cttggggatg ctgccagcgc
     6661 ggccgccctg ggcaccgtcg ttggggcaga catgacagaa cgcgagatcg tctggctaat
     6721 gcgcaccgag tgggctcgat cggcggacga tatcctctgg cggcggagca aactgggtct
     6781 gcgctttgat ggcacgcagc gcgcgaagct ggaggcgctg gtggcaaaag ctggttcctc
     6841 ggccgcgcat tctcctgggg cgtcgatacc acggacagcc tgatgcggtg cggacaatac
     6901 gcccgccgat tggacatccg agcctggata acccattttc cggagatggt tgggaggaca
     6961 cgcgtcatga gcgcatcaga cagtcgatac accgcgcaga acctgatgac cgtgcatggc
     7021 ctgcgcgccc aggcggttgt gcatgaacgg atcgccgaag cgcgtcagca gggcgataca
     7081 tcggggctgg agcgttggca gaacgtcgaa gcggcaatca gcgaactacg caaaaccgcg
     7141 cctcgggatg cttccaaggg gtgactggct ggggcgggag ggatcgaacc tccgtatgac
     7201 ggaatcaaaa tccgttgcct taccgcttgg cgacgcccca acgtccggac gtgataggtc
     7261 ggtcgccggc ctgcgtaaag ccagaaccgc tactggtcgt tcattccccc accccagacc
     7321 caccagccat cgcgcacgat accgccagta gcccggcgcg cggcttccgg cgtgtcgaac
     7381 agtccgaagc aagtggcgcc tgagccactc atgcgcgcca gccggcagcc gggcgtggcg
     7441 gccagtgcat ccagtgcggt tccgattgcc ggcgccagca tgcgggcagg tcgttcaagg
     7501 tcgttccggg tggcagcaag gtcggcgacg agagctcgga catcccgcca accatgggca
     7561 ggaaacccgg ctcgacctga aaactcgcct gatcgggcac gaaaaaccga cggcgtcgag
     7621 accgccacgc caggattgac caggacaagg cctgccaccg gcaatgatgg agccggcacc
     7681 aacacctcgc caacccccga catcaacgct gggcgggcaa caaggcaaac tttcacatcc
     7741 gcacctagat cacaggccag ttgggcaaga tcctccacgg acggttcgat ccgccacaac
     7801 cggcacagca gccgcaacgc cgccgccgcg tccgccgatc cgccaccgat accggatgcg
     7861 accggaagat ttttctcaag caccagtctc gccaacgcgg ggcacccagc acgagcggcg
     7921 agtgctcggg cagcccgcag caccaggtta tcagcgtcgg cgtcgagggc ggtggcgaaa
     7981 ggcccggtca ccgacagcga tagcgc
//
LOCUS       DASWQN010000071         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_930979, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000071 DASWQN010000000
VERSION     DASWQN010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930979"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1617
                     /locus_tag="VGC82_01210"
     CDS             241..1617
                     /locus_tag="VGC82_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0421923.1"
                     /translation="MPVEAVIAAARDFLGDRITVNAALRDHHSHGQDTQPPVLPDAVA
                     FVETTEETCRLMALCYAEHVPVVPWGAGTSLEGHVTPVRGGITIDLSRMNRVLDISQA
                     DMDCRVEAGVTRDQLNTHLRDVGLFFPVDPGTSACTIGGMSATRASGTNAVRYGTMRD
                     NVMGLTVVMPDGRLIRTGGRVRKSSSGYDLTRLFVGSEGTLGLITEIQLRLHGIPEAV
                     SSATCQFQTLRDAVETVIAILQIGIPVARMELLDDVQMAACIAYSKLEGLAALPTLFF
                     EFHGTPAGVAEQAGQAEEIALGNEGLGFAWATEPASRTKLWQARHDAYWAALAWKPGH
                     RAIATDAIVPISRLNEAILGAKEDIAASGLTAPIVGHVGDGNFHTVIMVPPEPDGMAR
                     AWELDKKIVARALSLGGSCSGEHGVGIGKREFLEQEHGAEAMAVMRAVKQALDPRGVM
                     NPGKIFLN"
BASE COUNT          417 a          626 c          683 g          405 t
ORIGIN      
        1 tcgagttcga acagacggtc gtaaaacaga gcggcgatcc gcggggcgat cggcaccacc
       61 ttagcaaacg aatcgcggac gagtgcctga ttttccggcg tcatagggaa cccctgttgt
      121 tgactcggac cttatgcaaa aggtgcacct gtgagcaaga agtggaccgt cggccgaagc
      181 gcttgccacc acatcggtcg gggtccaatt ctgtgcctaa cccgcagccg agagatatcc
      241 atgcctgtcg aagccgtcat cgccgccgcc cgggacttcc tcggcgatcg cattactgtc
      301 aacgcggcat tgcgcgacca ccactcgcac ggccaggaca cgcagccgcc ggttctgccg
      361 gacgcggtcg cattcgttga aaccactgaa gaaacttgcc ggctgatggc cctgtgttac
      421 gccgaacatg tgcccgtcgt gccctggggc gccggaacct cattggaagg ccacgtaacc
      481 ccggtccgcg gcggcatcac gatcgatctt tcgcgcatga accgggttct ggatatcagc
      541 caggcggaca tggattgccg ggtggaggcc ggcgtgacgc gggaccagtt gaacacccac
      601 ctgcgcgacg tcgggctgtt ctttcccgtc gatcccggca cctcggcctg cacgatcggc
      661 ggcatgtccg ccacccgagc atcgggcacc aatgcggttc gatatggcac gatgcgagac
      721 aacgtgatgg gcctgacggt ggtgatgccc gacgggcggt tgatccgcac cggtgggcgg
      781 gtgcgcaaat cgtcctcggg ctacgatctg acacgtttgt tcgtcgggtc cgaggggacg
      841 ctaggcctta ttaccgagat tcagctccgg ctgcacggca ttcccgaagc ggtgtcatcc
      901 gcgacctgcc agttccagac gctgcgcgac gcggtggaga cggtcatcgc aattctgcag
      961 atcggtatcc cggtcgcgcg gatggaactg ctcgacgacg tgcagatggc cgcctgcatc
     1021 gcttattcga agctggaagg acttgccgcg ctgccgacgc tgttcttcga gtttcacggc
     1081 acgccggccg gcgtcgccga acaggccggt caggccgagg aaatcgcgct cggcaatgaa
     1141 ggcctcggtt tcgcctgggc caccgaaccc gcttcccgaa cgaagctgtg gcaggcgagg
     1201 cacgatgcgt attgggcggc cctggcctgg aagcccgggc atcgggcgat cgcgacagac
     1261 gcgatcgtgc cgatttcccg gctgaacgag gcgatcctgg gagcgaagga agatattgcc
     1321 gcctccggcc tgaccgcgcc gatcgtcggg catgtcgggg atggcaactt ccacaccgtg
     1381 atcatggtgc cgccggagcc ggacggcatg gcgcgggcct gggaattgga caagaagatc
     1441 gtcgcgcggg cgctctccct cggcgggtca tgcagcggcg agcacggtgt cggtattggc
     1501 aagcgcgaat tcctggagca ggagcatggt gccgaggcga tggctgtgat gcgggcggtg
     1561 aagcaggcgc tggacccgcg gggggtgatg aatcccggga agatttttct gaattagggc
     1621 cgcagccgga ggatcgatgt attgactgag caatgattct aacttcttgg cactaatact
     1681 gctggtcagt caccctccga atggaccgcc ttcatggcaa gcagacgtcg atggcggctc
     1741 accctgagga caggcggtca gttggcatgg aggtcttttg ttccgaaatc gttactatcg
     1801 cagagatcac ggaatctgag acgaagtgag acgtccataa atgggctttt tactgcatga
     1861 gtccggcgcg ttggattatc ctcgaccata gtacccgatg ctgtggcgta tggttggata
     1921 aagcgctgcg ataattcaaa aaatcggcgt tcaaccctga caaggatgag ctcgaaatga
     1981 aggaatgcca cgcagcagcc ttattcaagc tgattgtcga ccctatccgg ctagcgcgat
     2041 caagatgcga aacccttggc tggttgatcg ccagcatcct gacagccgga accgttactc
     2101 tgacccggtt agcacctcac attgacagcg c
//
LOCUS       DASWQN010000072         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1591278, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000072 DASWQN010000000
VERSION     DASWQN010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591278"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /gene="sufD"
                     /locus_tag="VGC82_01215"
     CDS             <1..843
                     /gene="sufD"
                     /locus_tag="VGC82_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY0421924.1"
                     /translation="DGAALHVPAGFDAGLVQLVSIAGGAADFHPRHSIRLSQGARLTV
                     LEISAGEGAYLLNTVAEIHVAEGAHLTHVRLQDEAPTAFHVSTTYVDVAAGGTYDSFT
                     LNLGARLSRTEVHAQLSGKGAMAHLNAAQILAGSQHGDFTTVVGHTAPGCTSRQTVKN
                     VLAGRSRGVFQGKIEVARHAQKTDGYQMNQALLLSSDAEVNSKPELEIFADDVKCSHG
                     ATVGELDAEQMFYLRSRGIPETEARSILVRAFLAEALDAVADETIRRRLDSAVEQWWE
                     RQEV"
     gene            849..>1573
                     /locus_tag="VGC82_01220"
     CDS             849..>1573
                     /locus_tag="VGC82_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0421925.1"
                     /translation="MTNPVALDVERIRRDFPILTQTIRGKKLVFLDSAASAQKPRAVI
                     DAMVYAMENQYANVHRGLHWMSERTTESYEAARDAAAALMNAGDRSEIVFTRNSTEAI
                     NLVAHSYGRGVLKRGQAVVISGMEHHSNIIPWQLLRDAHGIEMRVAPVTDAGELDMAA
                     YEALLEDGKVGLVAITHMSNVLGTVVPAERVVQIAHAHGAKVLFDGSQAIVHRKVDVR
                     ALGCDFYAWTGHKLYGPTGIGVL"
BASE COUNT          304 a          464 c          504 g          301 t
ORIGIN      
        1 gacggcgccg cgctgcatgt gccggcggga tttgatgccg gcctggtgca gcttgtcagc
       61 atcgcgggtg gcgccgccga ttttcatccg cgtcacagca ttcgcctgag ccaaggcgcg
      121 cgtctgaccg ttctggaaat atccgccggc gaaggcgcct atctgctcaa cacggtcgcc
      181 gagattcatg tcgcagaggg tgcgcacctg actcatgtcc ggctgcagga tgaagcgccg
      241 actgccttcc atgtctccac gacctacgtg gatgtggcag ccggcggcac ctatgacagc
      301 tttacgctca atctgggtgc gcggctttcg cggaccgagg ttcatgcgca actgagcggc
      361 aagggcgcga tggcccatct taacgcggcg caaattcttg ccgggtcgca gcacggcgat
      421 ttcaccaccg ttgttggaca tactgcaccg ggatgcacat cgcgccagac ggtcaagaat
      481 gtcctcgccg gccgctcccg cggggtcttt caaggcaaga tcgaggttgc tcggcatgct
      541 cagaagaccg acggttacca gatgaaccag gcactgctgc tgtcgtccga tgccgaggtc
      601 aactcgaaac cggaactgga gatcttcgcc gacgacgtaa aatgtagtca tggcgcgact
      661 gttggcgaac tggatgcgga gcagatgttc tatctgcgca gtcgcggcat tcccgaaaca
      721 gaggctcgat ccattctggt gcgggccttt ctcgcggaag cattggatgc cgttgccgat
      781 gagacgatcc gaagacggct cgattccgcc gtggagcagt ggtgggagag gcaggaagta
      841 tgaatgtgat gaccaatcct gttgccctgg acgtcgagcg tatccggcgc gacttcccga
      901 ttctgacgca aaccatccgc ggcaagaagc tggtgttcct tgacagcgcc gcttcggccc
      961 agaagccgcg tgcggtgatc gatgccatgg tgtacgccat ggaaaatcaa tatgccaacg
     1021 tccatcgcgg cttgcactgg atgagcgagc ggacgaccga atcctatgaa gccgcccgcg
     1081 atgccgctgc cgccttgatg aacgccggcg accgttcgga gatcgtattt acgcgcaaca
     1141 gcaccgaagc gatcaacttg gtagcccaca gctatgggcg cggggtgctg aagcgaggcc
     1201 aggccgtggt gatttcaggg atggagcacc actccaatat cattccctgg cagctgctgc
     1261 gggatgcgca tggcatcgag atgcgggtcg cacctgtgac cgatgcgggt gaactcgaca
     1321 tggcggcgta cgaagcgttg ctggaagacg gaaaagtcgg gctggtggca attacccaca
     1381 tgtcgaacgt gctgggcacc gtcgtcccgg ccgaacgagt ggtccagatc gcccatgccc
     1441 atggcgccaa ggtgttgttc gacggatcgc aggccatcgt gcaccgcaag gtcgatgtcc
     1501 gcgccctcgg gtgcgacttc tatgcctgga ccggccacaa gctctacggt ccgaccggga
     1561 tcggcgtgct gtg
//
LOCUS       DASWQN010000073         3819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_799758, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000073 DASWQN010000000
VERSION     DASWQN010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799758"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VGC82_01225"
     CDS             complement(<1..816)
                     /locus_tag="VGC82_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0421926.1"
                     /translation="MGAILVRRLLGALPILLIVSLITFGMIHLIPGDPAAAIAGMSAT
                     PEQIANIRRDLGLNEPLPAQLFHWYVNLLHGDLGRSLLLGQPVMQATMQRLPVTLALS
                     AYALVLTLLFGLASGIVAALRQNTWVDQAAMVLAMLGISVPSFYLGLLLIIVFAVDLR
                     WLPTGGYIALTDDPVGWLATSTMPAISLALLLAGLLARITRSTMLEVLRQDYIRTARA
                     KGLANRTVVMKHALANALIPIVTVVGIIVSLLISGSVVVETLISIPGIGQLLTQ"
     gene            complement(886..2460)
                     /locus_tag="VGC82_01230"
     CDS             complement(886..2460)
                     /locus_tag="VGC82_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0421927.1"
                     /translation="MKSGFLAPVELILAAGLSVAAARAEPFKCPHVGGNFVFGQEANI
                     NSLDQMTSATISTRNIAMNIYESLMTRDENNHPILELAEAMIEAPDHLTYTFKLRQGV
                     HFHNGKAMTSADVVASFDRYGKIGNQRSMLSNVDGWDAPDAATFVIHMKKIQPTFLEA
                     LSSFSVPIVIIPAENRDVPAQQLTQPIGTGPYQLVESVPGSMVKLKRFEAYRPNTSFQ
                     DRTGFGGYKQACFDTVTFRIVTEPGARVAGLRTGELQGVEDVPSRSVPELKQDSRIRI
                     LPLANWWIQIANPNTSNPPTDKLLVRQAIQAALDMDEIMDAASDGNYQLNVGFQYPNQ
                     PDYTDAGKETYNLHNPELAKKYLVQAGYQGEPVVLLTNKDYPPMYNSALVMQQQLQAV
                     GINAQMKVVDWPTSVQMSLNTTEGWNFHFTGWGTQPALGGLATMQFLVQPNATYKPQE
                     GKDDPDILAAWNDMNTLPTAAGRQQAFAAMQKLVLERVYAIPFGSFTKVQAVRSNVNG
                     FVPFRIPRMTNVWFDR"
     gene            complement(2875..>3819)
                     /locus_tag="VGC82_01235"
     CDS             complement(2875..>3819)
                     /locus_tag="VGC82_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421928.1"
                     /translation="IVTGTTAAITDASGNKWTITTGGQVAINGKADTTTGSVIELAYV
                     GGKIWQENSSKLWWGETLPNASWAPTAGTATSPLPVPINPSANNTMVTGTTAAITDAS
                     GNKWTITTGGQVAINGKADTTTGSVIELAYVGGKIWQENSSKLWWGETLPNASWAPTA
                     GTATSPLPAPITIAATQTSATISASRVSVTSTSGNHMVFISGTGDIVALAGGTNTITD
                     TGKGNTYVIPAAGKGYDNFTSNILNTGDTLDLRTALAATNWTGTTATLAKYLSVVDNS
                     QGAVLSIAPTSGGAGVAIATINGATTASLTTLLAHSIA"
BASE COUNT          670 a         1191 c         1218 g          740 t
ORIGIN      
        1 ctgcgtcagc agttggccga tgcctggaat ggaaatcagc gtctccacga caacggaacc
       61 cgagatcagc aggctgacga tgatcccgac aacggtgacg atcgggatca gcgcgttggc
      121 cagcgcgtgc ttcatcacca ccgtccggtt cgccagcccc ttggctcggg cggtgcggat
      181 gtagtcctgg cgcagaacct ccagcatcgt cgagcgcgtg atgcgcgcca gcaggccggc
      241 caacagcaac gccagggaga tcgccggcat ggtggacgtc gccagccagc caaccggatc
      301 atcggtcagc gcgatgtatc ctccggtcgg tagccagcgc agatcgaccg cgaacacgat
      361 gatcaacagc aacccgaggt aaaagctcgg taccgagatc cccagcatcg ccagcaccat
      421 ggcggcctga tcgacccagg tgttctgccg cagcgccgcg acgatgccgc tggccaagcc
      481 gaacagcaag gtcagcacca gcgcgtatgc ggacaaagcc agggtcacgg gcaaacgctg
      541 catcgtcgcc tgcatcaccg gctggccaag cagcagcgaa cgaccgaggt cgccatgcag
      601 caggttgacg taccagtgaa aaagctgcgc cggcagcggc tcgttcaggc caagatcacg
      661 gcggatgttg gcgatctgct ccggcgtcgc ggacatcccg gcgattgccg ccgccggatc
      721 gccggggatc aggtggatca tcccgaacgt gatcaggctg acgatcagca ggatcggcaa
      781 ggcgcccagc aaccggcgaa ccaggatggc ccccatcagg cgcctggccc cccgcggaac
      841 gtcaattcgc ggaacgtcat gcgcgcaacg ccgctgcgcc gagcgtcaac gatcgaacca
      901 gacgttcgtc atcctgggaa tccggaacgg aacgaacccg ttcacattgg aacggacagc
      961 ctggaccttg gtgaaggagc cgaacgggat ggcgtaaacc cgctccagca ccagcttctg
     1021 catggcagcg aacgcttgtt gccggcccgc ggcggtcggc agcgtgttca tgtcgttcca
     1081 ggcagccagg atgtcgggat cgtccttgcc ctcctggggc ttgtaggtcg cattcggctg
     1141 caccaggaac tgcatcgtcg ccagcccacc caatgctggc tgcgtgcccc agccggtgaa
     1201 gtggaagttc cagccctcgg tggtgttcag cgacatctgc accgaggtgg gccagtccac
     1261 gaccttcatc tgcgcgttga tgccgaccgc ctgaagctgc tgctgcatca ccagcgccga
     1321 gttgtacatc ggcgggtaat ccttgttggt cagcaacacc accggctcgc cctgatatcc
     1381 cgcctgcaca aggtatttct tcgccagttc cggattatgc aggttgtagg tctccttgcc
     1441 ggcatccgtg tagtcgggct ggttggggta ctggaagcca acattcagtt ggtagttgcc
     1501 atccgacgcg gcgtccatga tctcgtccat gtccagcgcc gcctggatcg cctggcgcac
     1561 cagcaacttg tcggtcggcg ggttcgaggt attgggattg gcgatctgga tccaccagtt
     1621 ggccagcggc aaaatccgga tcctgctgtc ctgcttcagc tccggcaccg atctgcttgg
     1681 cacatcctcg accccctgca gctcaccggt ccgcaagccg gcgacgcggg caccaggttc
     1741 agttacgata cggaacgtca ccgtgtcgaa gcaggcctgc ttgtagccgc cgaagccggt
     1801 gcggtcctga aagctggtgt tgggcctgta ggcctcgaag cgcttcagtt tcaccatgct
     1861 gcccggcacc gactcgacca gctggtacgg cccggtgccg atcggttggg taagctgctg
     1921 ggcgggaacg tcgcggttct cggccggaat gatcacgatc ggcacgctga aggacgacag
     1981 cgcctcgagg aaggtcggct ggattttctt catgtggatc acgaaggtcg cggcgtccgg
     2041 cgcatcccag ccgtccacgt tgctcaacat gctgcgctgg ttgccgatct ttccgtatcg
     2101 atcgaacgac gccaccacgt cggccgaggt catcgccttg ccgttatgga aatggacgcc
     2161 ctggcgcagc ttgaacgtgt aggtcaggtg gtccggcgct tcgatcatcg cctcggccag
     2221 ttcaaggatc ggatggttgt tctcgtccct cgtcatcagc gactcgtaga tgttcatcgc
     2281 gatgttgcgg gtggaaatcg tggccgacgt catctggtcc aggctgttga tattggcttc
     2341 ctgaccgaag acgaaattgc cgccgacatg cgggcatttg aacggttcag cccgcgccgc
     2401 ggcgacggaa aggccggcgg caagaatcag ttcgacaggg gcaagaaatc cgcttttcat
     2461 ttatctgtcc cccggctggt aggaacaccc ttctgcggac ttagggttgg tccgtcaata
     2521 aatgcttcga gccgagcgtg cgccggtaga tccctcatgg tgggccttcg cccagggggt
     2581 gtttggatca tgcaccgatt ggggcggcta gtcatcaccg gacccttgtg ccgcgtgcag
     2641 tcaagtttgg tcacgcaggt ccgggacctt tgggatcaaa ccgactgccg agatcaaact
     2701 ggctgcgcgg attgcggcgc aacaatgcgg ccgggatcga acagcgggat cgcgggcatc
     2761 gccatgatcg aactgaagtc ccgcctggcc gctgggaaag tgcgggcagg ctgcatcaac
     2821 ggcggacgaa cgcaccgcca ggggagattt ccccggcggt gcgcctgccg atgctcaggc
     2881 gatcgaatgc gccagcagcg tcgtcaaact cgccgtcgta gccccgttga tcgtggcaat
     2941 cgccacaccg gcgccgcccg aggttggcgc gatcgacaga acggcccctt ggctgttgtc
     3001 aacgacgctg agatatttcg ccaacgtcgc ggtcgttcct gtccagttcg tcgccgccag
     3061 cgcagtccgc agatcgagcg tatcgccagt gttcaggata ttgctggtaa aattgtcata
     3121 tcccttgccg gcggccggaa tgacataggt gttgcccttg ccggtatcgg tgatcgtgtt
     3181 ggtgcctccc gcgagagcga cgatgtcgcc ggtgccgctg atgaacacca tgtggttgcc
     3241 ggatgtcgat gtcaccgaaa cccgactggc actgatcgtg gcgctggtct gtgtggcggc
     3301 tatcgtaata ggggcgggca acgggctggt ggcggttccg gcagtcggcg cccaggacgc
     3361 gttcggcagg gtctcgcccc accaaagctt gctgctgttc tcctgccaga tcttgccgcc
     3421 cacataggcc agttcgataa cgctgccggt ggtggtatcg gccttgccgt tgatcgccac
     3481 ctgaccgccg gtggtgatgg tccatttgtt gccgctggcg tcggtgatcg ccgcggtcgt
     3541 tcccgtcacc atcgtgttgt tggccgacgg gttgatcggc accggcaacg ggctggtggc
     3601 ggttccggcg gtcggcgccc aggacgcgtt cggcagggtc tcgccccacc aaagcttgct
     3661 gctgttctcc tgccagatct tgccgcccac ataggccagt tcgataacgc tcccggtggt
     3721 ggtatcggcc ttgccgttga tcgccacctg gccaccggtg gtgatggtcc atttgttgcc
     3781 gctggcgtcg gtgatcgccg cggtcgttcc ggtcacgat
//
LOCUS       DASWQN010000074         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_7952, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000074 DASWQN010000000
VERSION     DASWQN010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7952"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VGC82_01240"
     CDS             <1..348
                     /locus_tag="VGC82_01240"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY0421929.1"
                     /translation="AAPLVFRSWQPGERLVPGVWSIPVPADGEVVQPDVLLVPLVGYD
                     HQAFRLGYGGGFYDRTLAAMPHRPTTLGVGFSLASLPTVHPQPHDIPMDVIVTEQQST
                     VRVPDSLGPSPDP"
     gene            361..828
                     /locus_tag="VGC82_01245"
     CDS             361..828
                     /locus_tag="VGC82_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0421930.1"
                     /translation="MQPGMEHFAENLLAGMSEAIVHADANGVIRFWNRGATRIFGFNE
                     TEALGRSLDIIIPEGLRPRHWQGYRTTMQTGQSRYGDGHLLSVPAVRKDGSRISVDFT
                     IVPFTDDAGHMTGIAAIMRDGTARFEELRALRKGLAARSASASASGPGSTPNR"
     gene            complement(927..1244)
                     /locus_tag="VGC82_01250"
     CDS             complement(927..1244)
                     /locus_tag="VGC82_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421931.1"
                     /translation="MDLQTLLLLVAFRNRIREPDLDSGLSRFVEVVGRNIDPEDFREA
                     LSRALAAGDIHDPVQLPAGALQCHWHLSLTSTGVSKVRALLRAHPALGTSCSGHMVNP
                     PMN"
     gene            complement(1515..>2687)
                     /locus_tag="VGC82_01255"
     CDS             complement(1515..>2687)
                     /locus_tag="VGC82_01255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0421932.1"
                     /translation="LDVTPTERRTLLFSATIPREIAALAKRYQHEALRIAATGEGQAH
                     RDIEYRAITVAPRETDRALVNILRWYNERALVFCATRAAVTRLHGNLTERGFSAVALS
                     GELTQAERNRALQALRDRRAQVCVATDVAARGIDLPDLGLVVHAELPRDKDTLLHRSG
                     RTGRAGRKGTAVLLVPYPRRRVAERLLANAHVRATWAAAPSAEEIHGRESERLVKEAI
                     AAAETASAEPELVALGRSVLEAQGAEAVAAALVRALGASLPAPEELEMPQAESQLQAP
                     PNPRAPVRRRDGDGTDEGIWFRLNVGHNRNADPRWLLPFLCRRGHVTRQEVGRIQVME
                     RETRVEIASWAAPRFTDAARHQEADDEDIRIEPMRGPAIPHGRGGARHTRAAAPRR"
BASE COUNT          433 a          899 c          880 g          475 t
ORIGIN      
        1 gcggccccgc ttgtctttcg ctcctggcaa cccggtgagc gtctggtgcc cggcgtatgg
       61 agcattccgg tgcccgcgga tggagaggtc gtgcagcctg acgttttgct ggtgccgctg
      121 gtcggctacg accaccaggc attccggctt ggctacggcg gtggcttcta cgaccggacg
      181 ctggccgcca tgccgcatcg gccaaccaca ttgggggtag gcttcagcct ggcgtcgctt
      241 cccaccgttc accctcagcc acacgatatc ccgatggatg tcatcgttac cgaacaacaa
      301 tcgacggtga gggtgccgga ttcgcttggc ccttcgcccg atccatagcg gaggaagtcg
      361 atgcaacctg gaatggagca tttcgccgaa aatctgctgg ccggaatgtc cgaagcgatc
      421 gtccatgcgg acgcgaacgg cgtgatccgg ttttggaacc gcggggcaac ccgaattttc
      481 ggcttcaacg agacggaagc gctaggccga tcgctcgaca tcatcattcc tgaaggcctg
      541 cgcccgcggc actggcaagg ctaccggacc accatgcaaa ccggtcagtc gcgttacggg
      601 gacggccatc ttttgtcggt gcctgcggtg cggaaggatg gctcgcggat ctcggttgac
      661 ttcacgatag tgccatttac tgacgacgca gggcacatga ccggaatcgc cgcgatcatg
      721 cgggacggca cggcgcggtt cgaggagttg cgcgcgctgc gcaagggact cgcggcgcga
      781 tcagcgtctg cgtctgcctc tgggccgggc agcacgccaa accgctgagc cgcggccggc
      841 ctgatcgcag caccgccgga cacgccctgc gacttgttta caggccatgg gccacggaca
      901 gcactattct gccagtttgg cggcggttaa ttcatcggcg gattcaccat gtgcccagag
      961 caggatgtac ccaaagcagg atgtgcccga agcagggccc gcaccttgct cacgcctgtg
     1021 gatgtgaggc tgaggtgcca atgacactga agagccccag ccggaagctg caccgggtca
     1081 tgaatatccc ccgccgccag cgcacgggat agtgcctcgc gaaaatcctc agggtcgatg
     1141 ttccttccga cgacttccac gaagcgcgac aggccgctgt cgagatcagg ttcacgaatc
     1201 ctgttccgga aggcaaccaa cagaagaagc gtctgcaaat ccatgcaacc cagcctgtcc
     1261 tgaaccggct tcgccgtcca gatgacgcat ggcgcgctgg gtccaacttc gccggcacaa
     1321 ggtctgcacc gagcagatca ggcaaagcct ccaggccgat caggctacgt ttccggcacc
     1381 gttgcggcgg ccgatccggc gccggccttc gccaatgcgt ggcagccggt cgcacccatg
     1441 gcgtccgcgg ccttgggcct gtgccagttt tgggcctgtg ccagttttgt cggatcttcc
     1501 ggccgagcat tggatcagcg gcgtggggcc gctgcacgcg tgtgccgcgc gccgccgcgc
     1561 ccgtggggta tggctggccc acgcatcggt tcaatccgga tgtcctcgtc atccgcttcc
     1621 tggtggcggg cggcatcggt gaagcggggc gcggcccagg aggcgatttc cacccgcgtt
     1681 tcgcgctcca tcacctggat gcgtccgact tcctgacgcg tcacatgccc ccggcggcaa
     1741 aggaagggca gcagccagcg tggatcggcg ttacggttgt gcccgacatt gaggcggaac
     1801 caaatccctt cgtcggtgcc atcgccatcg cgcctgcgca ccggcgcgcg cgggttcggc
     1861 ggggcttgta gctgggactc cgcctgcggc atctccagtt cctccggtgc gggcaaggac
     1921 gcgccgaggg ctcgtaccaa cgctgccgcg acggcttccg caccctgcgc ttccaggacc
     1981 gagcggccca acgcaaccag ttcgggttcc gcggatgcgg tctcggccgc cgcgatcgcc
     2041 tccttgacca accgctcgct ctcgcggccg tggatctctt ccgccgaggg cgccgctgcc
     2101 caggttgcgc gaacgtgggc gttcgccagc agccgctccg cgacccggcg gcgcggatac
     2161 ggcaccagca gtactgcggt gcccttgcgg cccgcacggc cggtacggcc gcttcggtgc
     2221 agcaatgtgt ccttgtcgcg cggcaattcg gcatgcacca ccagcccgag atcgggcagg
     2281 tcgatcccgc gtgccgccac gtcggtggca acacaaacct gggcgcggcg atcgcgtagc
     2341 gcttgcaggg cgcggttgcg ttctgcctgg gtcagctcac cggaaagggc aaccgcgctg
     2401 aagccgcgct ccgtcaggtt tccatgcaac cgggtcacgg ctgcccgggt ggcacagaac
     2461 acgagggcgc gctcattgta ccagcgcagg atgttgacca gcgcccggtc ggtttcccgt
     2521 ggtgcgacgg tgatggcgcg gtactcgata tcgcgatgcg cctgcccctc cccggtcgcc
     2581 gctatgcgca acgcctcgtg ctggtagcgc tttgccaatg ccgcgatctc gcgcgggatc
     2641 gttgcggaaa acagcagcgt ccggcgctcg gtcggcgtga cgtccag
//
LOCUS       DASWQN010000075         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_667728, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000075 DASWQN010000000
VERSION     DASWQN010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667728"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VGC82_01260"
     CDS             complement(<1..743)
                     /locus_tag="VGC82_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HEY0421933.1"
                     /translation="MRTQYSLTRRLAMLGMVGVAVTRKQLLAQPASASDAWSILAAQI
                     FNNRPIGDGRAVLQIEAPYRAEDAAVVPITIRIVPPAADARSVDQITLVIDENPSPVA
                     ATFTLGANSGIRSLSTRVRVDSYTNFHAVAALSGGELCAVDRFVKAAGGCSAPAAKQE
                     ADSIPLGTMRFRQFSGDPTATTREVQLLIRHPNYSGMQMNQVTRLYVPAHFVSSVRIW
                     QGDELLLGVESGISISENPEFRFDYRPNGA"
     gene            1039..1929
                     /gene="pqqB"
                     /locus_tag="VGC82_01265"
     CDS             1039..1929
                     /gene="pqqB"
                     /locus_tag="VGC82_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HEY0421934.1"
                     /translation="MAVVLGSAAGGGFPQWNCGCHLCRLVRQRDPRVQAATQASVAIT
                     GDGVDWLLVGASPDIRQQLSQTACLWPRTSTRDSPVRGVVLTGGDIDAIAGLLVLRER
                     QPFVIYAPRPVLDLLADNDVFNVLDPALVQRVEVTPMQPISCGRGLTLTLLPMPGKVP
                     LYREDRNAAAAEAGSAYAALVEAAGHTIVVALACAEITDAVLANLRRADVLFFDGTLF
                     TDNEMIAAGLSEKTGARMGHVSISGPQGTLARLADLPARRILLHINNTNPILLRDSPE
                     RRQVEAAGFEVAYDGMEVPL"
     gene            1926..2666
                     /gene="pqqC"
                     /locus_tag="VGC82_01270"
     CDS             1926..2666
                     /gene="pqqC"
                     /locus_tag="VGC82_01270"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEY0421935.1"
                     /translation="MSDLLTHAQLEARLREIGAERYHIRHPFHRMLHSGQCSRGQVQA
                     WALNRYYYQAMIPLKDASLIGRTEDQSLRREWRKRLEDHDGDEQRPGGIERWLKLTDG
                     LGLDRADVVSTRLLLPATRFAVQAYVHYVRERSMLEAVASCLTELFSPQIITERMSGM
                     LANYDYVTPDILSYFDKRPPQAERDATFALEWVKQHARTAEQQQAVLDTLKFKCDVLW
                     CMMDALHYSYVTPALVPPGAFVPRASDD"
     gene            2659..2937
                     /gene="pqqD"
                     /locus_tag="VGC82_01275"
     CDS             2659..2937
                     /gene="pqqD"
                     /locus_tag="VGC82_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEY0421936.1"
                     /translation="MTDPLVPERPKLAPGVRLHLDKTRDAWVLLSPERIIEAEGPASE
                     ILRRCDGAHTVSEIIDELAVVYAADRDVIARDVNDMLAELTGKRLLIA"
     gene            2934..>3491
                     /locus_tag="VGC82_01280"
     CDS             2934..>3491
                     /locus_tag="VGC82_01280"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0421937.1"
                     /translation="MTGPAPLGLLAELTHRCPLACPYCSNPLDLENRADELDAAARAR
                     VFQQAAAMGVLQIHLSGGEPASRRDLIEITQAASDAGLYTNLITSAIGLDADRLGQLK
                     AAGLDHVQVSIQDTDPAAADRVAGYDGAHARKQVFAQAVVGLGMALSVNAVIHRANIA
                     HLSVLVDLAASLGAGRVEIAHAQYYG"
BASE COUNT          653 a         1103 c         1084 g          651 t
ORIGIN      
        1 gccccgtttg gtcggtagtc gaaccggaat tccggattct ccgagatcga aatcccgctc
       61 tcgacgccca ggagcaactc gtccccctgc caaatccgga ccgacgagac gaaatgcgcc
      121 ggcacgtaaa gccgcgtcac ctggttcatc tgcatcccgg agtaattcgg gtgcctgatc
      181 agcaattgca cctcacgcgt ggtcgcggtg ggatcgccgg aaaactggcg gaaccgcatg
      241 gttccgagtg ggatcgaatc cgcctcctgc ttggcggctg gcgccgaaca tccgccagcc
      301 gccttaacga agcgatcgac cgcgcacagc tcgccgccgg acagcgcggc aaccgcatgg
      361 aaattggtat aggaatccac gcgaacacgg gtcgacaagg accggatgcc gctgttcgca
      421 cccagggtga aggtcgcggc aaccggtgac ggattttcat cgatcactaa ggtaatttga
      481 tccacggatc gtgcgtcggc agccggtggc acgatgcgga tggtgatagg gacgacagcc
      541 gcatcttcgg cacggtaagg cgcttctatc tgcagaacgg ccctgccgtc tccaattgga
      601 cgattgttga agatctgcgc tgccagaatc gaccatgcat cggacgccga cgctggctga
      661 gccaagagct gtttcctggt gacagccacg ccaaccatcc caagcatcgc caagcggcgc
      721 gtcagcgaat actgagttcg catgtttgtg atccctccca agctgcgcct cggcaaccca
      781 gcaggctata tctggcgcgt cggaaaaacc gctgcaagag gcggtgccgc gccctggcgg
      841 cgaggctccg attgcatccc gcggtcgcga cgcttagact gccaatcgct cgagaaaaca
      901 aggagacaac caatggcctg gacccgcccg attcttcgcg aaatctgcat cggcatggaa
      961 atcaatgggt atttcgctgc gcggatgtag ttcgcctctt ataaactgcc acgcatgacg
     1021 gagcgacacg ctgaaccgat ggccgttgtg ctcggttcag cagcaggtgg tggattcccc
     1081 cagtggaatt gcggatgcca cctgtgtcgg ctcgttagac agcgtgatcc gcgtgtgcaa
     1141 gcggccactc aggcgagcgt cgccatcacc ggcgatggag tggactggct gctggttggt
     1201 gcgtcgcctg atattcgcca gcaactctcc caaactgcgt gcttgtggcc ccggacatcg
     1261 acccgtgatt cgccggtccg cggggtggtg ctgaccggcg gggatatcga cgcgatcgcc
     1321 ggcctgctgg tgttgcgtga acgccagcca ttcgtgatct acgcgcctcg tccggtgctg
     1381 gacttgctcg ccgacaacga tgttttcaac gtgcttgacc cggcactggt gcagcgggtg
     1441 gaagtgacac cgatgcagcc gatctcatgc ggccgaggcc ttacgctgac gttgctgccg
     1501 atgccgggaa aagttccgct ctaccgtgag gaccggaacg ccgccgcggc ggaagccggt
     1561 tcggcctacg ctgccctggt cgaggcggct ggccacacca tcgtcgtggc actcgcttgc
     1621 gccgaaatta ccgacgcggt gcttgcgaac ctgcggcgag cggacgtgct gttcttcgac
     1681 ggcacgttgt tcaccgataa tgagatgatt gcggcgggct tgagcgagaa gaccggcgcc
     1741 cgcatggggc atgtctccat cagtggcccc cagggcaccc ttgcgcgtct tgccgatttg
     1801 ccggcgcggc gcattcttct gcatatcaac aacaccaatc caatcctgct gcgcgattcg
     1861 cccgaacgcc ggcaggtcga ggcggcgggc ttcgaggttg cctacgatgg catggaggtg
     1921 ccgctatgag cgatcttctg acccatgccc aactggaagc ccgattgcgc gagatcggcg
     1981 ccgagcgtta tcacatccga catcccttcc atcggatgct tcacagcggc caatgcagca
     2041 ggggccaggt ccaggcctgg gcgctcaatc ggtattacta ccaggccatg atcccactga
     2101 aggacgcgtc cctgataggg cgcaccgagg atcagtcgct tcgccgcgaa tggcgcaaac
     2161 gcctcgaaga ccacgatggc gacgaacaac ggccgggcgg gatcgagcgg tggcttaaac
     2221 tgaccgatgg cctgggtctc gatcgtgccg acgttgtctc cacacgcctt ctgttgccgg
     2281 ccacccgttt cgccgtgcag gcctatgtgc actacgtacg cgagcggagc atgctggaag
     2341 ccgttgcctc ctgcctgaca gaactgttct caccgcagat cattaccgaa cgcatgagcg
     2401 ggatgctcgc caactatgac tatgtgacgc ctgacatcct gtcgtatttc gacaaacggc
     2461 cgcctcaggc cgaacgggat gcgacgttcg cgctcgaatg ggtcaaacag cacgcgcgaa
     2521 cggccgaaca acaacaggcg gtgctggaca cgctcaagtt caagtgcgac gtactgtggt
     2581 gcatgatgga tgcactgcat tattcctatg tgaccccggc gctggtgcca cccggcgcct
     2641 tcgtgccgcg tgcgtccgat gactgacccg ctcgtgccgg aacgtcccaa actggcgccc
     2701 ggcgtccgtc tgcacctcga caagactcgt gatgcgtggg ttctgttgag tcccgaacgg
     2761 atcatcgagg ccgaaggacc tgccagcgaa attctccgcc gctgcgacgg cgcccacacc
     2821 gtctcggaaa tcatcgacga actcgcggtc gtttatgcgg ccgaccggga tgttatcgcg
     2881 cgggacgtca acgacatgct ggctgaactg accggcaagc ggctgctgat cgcatgacag
     2941 gcccggcccc gctcggtttg ctggcggaac tgacccaccg ctgtccgctg gcctgtccct
     3001 actgctctaa cccgcttgac ctggagaacc gggccgacga actggatgcc gccgcgaggg
     3061 cgagggtgtt ccagcaggcc gcggcgatgg gggtcctgca gatccatctc tcaggcggcg
     3121 agccggcatc ccgccgcgac ctgatcgaaa ttacccaagc ggccagtgac gccggacttt
     3181 acaccaacct gatcacctcc gcgatcgggc ttgatgccga caggctgggg cagctgaagg
     3241 cggcgggact ggatcatgtg caggtatcca tccaggatac cgatcccgcg gcggccgacc
     3301 gcgtcgccgg ctatgatggc gcacacgccc gcaagcaggt gttcgcgcag gccgttgtcg
     3361 gcctcgggat ggcattgtcg gtcaatgccg ttattcatcg cgccaacatc gcgcacctgt
     3421 cggtgctggt ggatttggct gcttccctgg gagcgggccg agtggagatt gcccatgccc
     3481 aatattatgg c
//
LOCUS       DASWQN010000076         4879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_8156, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000076 DASWQN010000000
VERSION     DASWQN010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8156"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..1451)
                     /locus_tag="VGC82_01285"
     CDS             complement(234..1451)
                     /locus_tag="VGC82_01285"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY0421938.1"
                     /translation="MAKIKVKTPVVELDGDEMTRIIWGFIKEKLILPYLDIDLKYYDL
                     GIEYRDQTDDQVTVDAANAIAQYGVGVKCATITPDEARVTEFGLKRMYRSPNGTLRNI
                     LDGTIFREPIICRNVPRLVPHWTQPIVIGRHAYGDVYRAVEMQIPGPGTVTMSYQPAD
                     GGPVRTLEVHKFTEGGGVAMSMHNTRVSIEGFARASFNYAIMRGWPLYFSTKNTILKT
                     YDGYFMEIFATIYENEFKAEFAKRGLTYEHRLIDDMVACALKWNGAYVWACKNYDGDV
                     QSDTVAQGFGSLGLMTSVLLSPDGTKVEAEAAHGTVTRHYRQHQQGKETSTNPIASIF
                     AWTRGLIYRGQFDNTPDVTAFAETLEKVCIETVESGHMTRDLSILIGPDQPWLNTQAF
                     LAKLDENLQKAMK"
     gene            1772..4831
                     /locus_tag="VGC82_01290"
     CDS             1772..4831
                     /locus_tag="VGC82_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEY0421939.1"
                     /translation="MKPDKHSLTAVGILIAAACLIALTWVGTTQAIHAQRTENTIRVT
                     ATLANQALTLAEQINRQILGLDQTLRILVRAWENNPRDFDLEAWRNQAVVLNGLSRDM
                     LLTDENGIIRQSSVNEAINQNASSLDYFRALADGAADNGDRMYIGPATIDGIMRQWHM
                     NVARALHYPDGSFAGAIDADYRIAAITDVFSQTDLGQGALVTLAGLEDGMLRGAVSSA
                     TIDPNVSVADTPMFAALQSADHGIWTGPSASDAVLRIHAFRQIPGRQLAVMVGMSEAE
                     AMRPAAIWRQQAQLFAGCITTLLVGLALLLIHGTRLARQREALILEDRAMLAGANAQL
                     EVARALAAAKAEQLEATLAGMSDGVSMIDAHMCLVEWNARFPEIAGVPAGILRVGLPM
                     EEILRSQVKTGQFGRVQDAEAEVERRMARLRVAPFGVAQRQRPDGHTLELRRNRLPDG
                     GFVTLYSDVTEHKLAEEALRKAQAAAEAANAEKSRFVAIVSHEIRTPLNALLNTVRLL
                     HDSDLSRAQQSLLAMARQSGDVLFGLINDILDMSQMEAGKLSIRPSLFELGPMLDGCV
                     EMFAGQAAQRGLSLHVSIAEGTPNTLLTDPGRLRQVQLNLLSNAVKYSNPGEVWLLAE
                     PGSGDHEAVRLTVKDNGPAIAPEARDRLFRPFARLERKGFDDAAGTGLGLSICHHLVT
                     LMGGTIGCDTWRSDEGREGNAFWSTLPATALPSLGADGAITPMADAESHLTPIDLSQR
                     QPHRARILLTEDIVANQVVTATLLRRLGHHVDIAANGPASVEAMKTTPYDLVFMDIFM
                     PGMSGQEATQIIRGLPEPACSTPIIALTANVAPEDEALFRAAGMNGLLGKPVSSAEML
                     AVLDKFASQAGHRRADFVPPPALNTAGPPALNTVKPPALNTVKPSAKTVLSAERINEL
                     RTNLAPATFVRLMEECLVDMDHRLPALRRALVAGSPGAVTAHAHAMVGMAAGYGMAAL
                     EDRLRQVMDAARIGDLSPLGPGIVAALEKDFEQAVRGLRDMLRTELV"
BASE COUNT          879 a         1547 c         1552 g          901 t
ORIGIN      
        1 gtcggtatga agctcttgcc cctcgacccg ttgggtggcc agcgtaaaac cgctgatcag
       61 cccggtcatc agcatgccgc ggcgcgccag tgggtaatcc cgcagacccg caagtccggt
      121 tacgtcgtcc atttccacct ccccggaata aagcatccgt cgcggcttcc ggcaggatcg
      181 aaaaatgcgg aacggatcat gcctgaaccg cgatccagaa gctgctttga acgctacttc
      241 atcgcctttt gcaaattttc gtccaacttt gccaagaacg cctgcgtgtt cagccacggt
      301 tggtcgggac cgatcaggat cgacaggtcg cgggtcatgt ggccggattc caccgtttcg
      361 atgcacactt tttccagcgt ctcggcaaag gcggtcacat cgggggtgtt gtcgaactgt
      421 ccgcgataga tcaggccgcg cgtccaggca aagatcgacg cgatcgggtt cgtggacgtt
      481 tccttgcctt gctggtgctg acgataatgc cgcgtgaccg tgccatgcgc cgcttccgct
      541 tccaccttcg ttccgtccgg gctgagcagc accgaggtca tcagccccaa tgacccgaac
      601 ccctgagcca cggtgtccga ctgcacatcg ccgtcgtagt tcttgcaggc ccagacatag
      661 gctccgttcc acttcaaggc gcaggctacc atgtcgtcga tcagccgatg ctcgtaggtc
      721 aggccgcgct tggcgaattc cgccttgaac tcgttttcat agatcgtggc gaagatttcc
      781 atgaagtaac cgtcgtaggt cttcaggatc gtgttcttgg tcgagaaata cagcggccag
      841 ccgcgcatga tggcatagtt gaaactggcc cgggcaaagc cctcgatgga cacgcgggtg
      901 ttgtgcatgg acatggcaac gccgccgccc tcggtgaact tgtgcacttc cagagttcgc
      961 actggcccgc cgtcagcggg ctggtagctc atggtgacgg tccccggacc cgggatctgc
     1021 atttccaccg cgcgatagac gtcgccgtac gcatggcggc cgatcacgat cggctgcgtc
     1081 cagtgcggca ccaggcgtgg cacgttccgg cagatgatcg gctcgcgaaa gatcgttcca
     1141 tcaaggatgt tgcgcagcgt gccgtttggg ctgcgataca tgcgtttcag gccaaattcg
     1201 gtcacccgag cctcatcggg cgtgatcgtg gcacatttga cgccgacgcc gtactgggcg
     1261 atggcgtttg ccgcgtcaac agtcacctga tcgtcggtct ggtcgcggta ctcgatgccg
     1321 agatcgtagt atttcagatc gatgtcgaga tagggcagga tcagcttttc cttgatgaac
     1381 ccccaaataa tgcgggtcat ctcatcccca tccagttcga caaccggggt cttaaccttg
     1441 atttttgcca tcatatcctc gtgggcggaa catagccggg accggttctc cggcctgccg
     1501 aaacgcccgg acaatcgcac cgcgcgttcc ccggggcaag ccggtgcctg tatccgccgc
     1561 tgcacaagtc gcccctgagg ggcgttggct gacggggcgt tggctgacgg ggcgttgggt
     1621 tcacccgccc ggcggcctgc cgcggccatg cccgccgagg aaaatcgctg aagctgcgta
     1681 actaagttga aattcgaagt gcgttacggc taggtaatgc cgccatggca ccgcccgagc
     1741 cgttacctaa actgaaattt ttgttcggat catgaaaccg gacaagcatt ccttaaccgc
     1801 cgtcgggatc ctcatcgctg ccgcttgtct gatcgcgctg acctgggtcg gcacgacgca
     1861 agccatccat gcgcaacgga cagagaatac gatccgcgtt accgcgacgt tggccaacca
     1921 ggcgctgacc ttggcggagc agatcaatcg gcagatcctc ggcctcgacc agaccctgcg
     1981 cattctcgtc agggcctggg agaacaatcc acgcgacttc gacctggagg cctggcgcaa
     2041 ccaggccgtg gtgctgaacg ggcttagccg ggacatgctg ctgaccgatg aaaacggcat
     2101 cattcggcaa tcctcggtga acgaggcgat caatcagaac gcttccagcc tggattattt
     2161 ccgcgcgctg gcggacgggg cggcggataa cggcgacagg atgtatatcg gtccggctac
     2221 gatcgacggc atcatgcgcc agtggcacat gaatgtagcg cgtgcgctgc actatccgga
     2281 cggatcgttc gccggcgcca tcgacgccga ctaccggatc gccgcgatca ccgacgtgtt
     2341 cagccagacc gacctgggtc agggcgcgtt ggtgacgctg gccggcctgg aggacggcat
     2401 gctgcgcggc gcggtcagtt cggccacgat cgaccccaac gtcagcgttg ccgacacgcc
     2461 gatgttcgcc gccctccagt cagccgacca cggcatctgg accggcccat cggccagcga
     2521 cgcggtgcta cggatccacg cgttccgcca gatccccggt cgccaactgg cggtcatggt
     2581 cggcatgagc gaagcggagg ccatgcggcc ggccgcgatc tggcgtcagc aggcgcagct
     2641 cttcgccggc tgcatcacca cactgctggt gggtctggcg ctgttgctga tccatggcac
     2701 gcggttggcg cgccagcgcg aggcgttgat cctggaagac cgagccatgc tggcgggtgc
     2761 caatgcgcag cttgaggttg cccgcgcgtt ggccgcggcg aaggcggagc agcttgaggc
     2821 gacgctggcg ggcatgagcg acggtgtgtc gatgatcgac gcccacatgt gcctggtgga
     2881 gtggaacgcc cgtttcccgg aaatcgccgg ggttcccgcc gggatcctgc gcgtcggcct
     2941 cccgatggag gagatccttc gctcccaggt gaaaaccgga cagttcggcc gggtgcagga
     3001 tgccgaggcc gaggtcgagc gccggatggc gcgcttgcgg gtcgcgccgt tcggcgtggc
     3061 gcagcgccag cgccctgatg gacacacgct tgaactgcgg cgcaaccggt tgccggatgg
     3121 cggcttcgtc acgctctatt cggatgtcac cgaacacaag ctcgccgagg aagcgctgcg
     3181 gaaagcccag gcggcggcag aggcggcgaa cgctgaaaag tcccgcttcg tggcgatcgt
     3241 cagccacgag atccgaacgc ccctgaacgc gctgctcaat accgttcggc tgctgcacga
     3301 cagcgatctg tcgcgggccc agcagtcgct gctggcgatg gcgcgccagt ccggcgacgt
     3361 gctgttcggg ctgatcaacg acattctcga tatgtcgcag atggaagccg gcaagttgtc
     3421 gatccggccg agcctgttcg agctgggccc gatgctggac ggttgtgtgg aaatgttcgc
     3481 gggccaggcg gcccaacgag gcctgtcgct gcacgtctcc atcgccgaag gcacgccgaa
     3541 caccctgctg accgaccctg gccggctacg ccaggtacag ctcaatctgt tgtcgaacgc
     3601 ggtaaaatat tccaacccgg gcgaggtctg gctgcttgcc gaaccaggca gcggcgatca
     3661 cgaggccgtc cgcctgactg taaaagacaa cggcccggca attgcgcccg aggcgcgtga
     3721 caggttgttc cggcctttcg cgcggctgga acggaagggc ttcgacgacg cggccggaac
     3781 cggcctgggc ctgtcgatct gccaccacct ggtgacgctg atggggggca cgatcggctg
     3841 cgacacctgg aggtcggacg aagggcggga aggcaatgcg ttctggagca ccttgccggc
     3901 cacggcgctg ccgtccttgg gcgccgatgg cgcaatcacc ccgatggcgg acgccgaatc
     3961 gcatttgaca ccgatcgacc tatctcagcg ccagccgcac cgcgcgcgga tcctgctgac
     4021 cgaggacatt gtcgcgaacc aggtggtcac cgcgaccctg ctgcgccgtc tgggtcatca
     4081 cgtcgatatc gcggcaaatg gtccagcttc cgtcgaagcg atgaagacga caccgtacga
     4141 cctggtgttc atggacatct tcatgcccgg catgagcggc caggaagcca cgcaaattat
     4201 ccgcgggctg cccgaacccg cttgctcgac cccgatcatc gccctgactg cgaatgtcgc
     4261 gcccgaggac gaagccttgt tcagggccgc cggtatgaat gggctgctcg gtaagccggt
     4321 atcatcggcc gaaatgttgg ctgtcctgga caagtttgcg tcgcaggcag gccatcgccg
     4381 cgcggatttc gttccgccgc ccgccttgaa caccgccggg ccacccgccc tgaacactgt
     4441 caagccaccc gccctgaaca ccgtcaagcc atcggccaag accgtgcttt ccgcggagcg
     4501 gatcaacgag ttgcggacca atctggcgcc cgcgaccttc gtcaggctga tggaagaatg
     4561 tctcgtggac atggaccacc gactgccggc gctacggcgc gcgctggtag cgggatcgcc
     4621 gggcgcggtc accgcccatg cgcatgccat ggtgggcatg gctgccggct acggcatggc
     4681 cgcgctggaa gatcggttgc gccaggtcat ggacgccgcc cggatcggcg acctaagccc
     4741 acttggtccc ggaatcgtgg cggcgctgga aaaggatttc gagcaggcag tcagaggctt
     4801 gcgcgacatg ctgcggaccg aactggtctg acgccgcgtt aacgggttgt ttacacctat
     4861 acttgtaggt tacccgcat
//
LOCUS       DASWQN010000077         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_140136, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000077 DASWQN010000000
VERSION     DASWQN010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140136"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1005)
                     /locus_tag="VGC82_01295"
     CDS             complement(184..1005)
                     /locus_tag="VGC82_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0421940.1"
                     /translation="MQTELVSIETETLPLDGALHLPDGPVTGSVLLFHGNTMNFYVGA
                     PRFLPPALTAMGFACLAFNRRGHDILSIRDSRTAEGAAFQLTHEGIEDNRIAAQWMAE
                     KGFRTPVVIGHSNGGMLAVPHVVAHPDTPAMVLLSAHGGGRDGVRRSSQSGLMAADRL
                     EEITAQAHAMVAAGRGKDLMLMPGWWYVITAESFLDRSSRMPDILELAPRITCPVLYL
                     RGDAESAENYPAEAFRDRAAGPCEVDIIRDCDHFYRGREAAVTARVTTWLAKTFL"
     gene            1004..1375
                     /locus_tag="VGC82_01300"
     CDS             1004..1375
                     /locus_tag="VGC82_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421941.1"
                     /translation="MAATSRRAQTVFVAKSAFHDLGKRFFRQRNRTAAANPIGSRIRK
                     CGGWVAIHQRAGGLQDQPTETDDAHIRRTKVLRRSIGDRTLAVLNAGVLFGHPLDPGV
                     AFSLLFGPVDQIIISRRRMGM"
     gene            complement(1339..>2337)
                     /locus_tag="VGC82_01305"
     CDS             complement(1339..>2337)
                     /locus_tag="VGC82_01305"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0421942.1"
                     /translation="GGNTMLTEADNILLTRTGPKTPMGDLFRRFWQPVLLSAQLPDRN
                     GPPLRLTVLGEDLRAFRVTDGSVGLVSPNCPHRGADLFFGRNEDGGIRCAYHGWKFAT
                     DGRCLAMPTMDPGSARDRAELNVRLLAYPVQEAGGFVWAYLGPRKHMPPLPELEFAML
                     PASQIYVSKKLQQCNWAQACEGGLDTAHFSFLHMAVSDADDVTSRLLTKVSSGTDTVR
                     WMRDDGAPRFTIADHPAGLVLGAARRGDPGQTYWRISQFLMPNHGLTPNACPGENYHG
                     QCWVAIDDTSCWIYNYTWNPERPLTNAERERFAAGSGIHARTDAGYIPIRRREMII"
BASE COUNT          430 a          712 c          761 g          434 t
ORIGIN      
        1 cggctgctgc gctgggggga cgaagccggg actacgacta aggaagcgaa acggcattgc
       61 agagtcggag ctgcaacgcc agggcatctt ctgcccgatc atacaggtgg aatatgcccc
      121 tcgtttgatt ttgtgcttct cgtgccgggg acgtcgcact cggcgagacc atgatccagt
      181 tccttacagg aacgttttgg cgagccaggt cgttacgcgg gcggttaccg ccgcttcccg
      241 gccgcggtag aaatgatcgc agtcgcggat gatatcgact tcgcacgggc cggcagcccg
      301 atcgcgaaac gcttccgcag ggtagttttc ggcactttcg gcatcgccgc gcaggtacaa
      361 taccgggcat gttatgcggg gagccaactc caggatatcc ggcatcctgg agctcctgtc
      421 gaggaaactt tccgcggtga tgacatacca ccaacctggc atcagcatca gatccttgcc
      481 ccggccggca gccaccatcg catgggcctg ggccgtgatt tcttccaagc gatcggcggc
      541 catcaggccc gactggcttg accgccgtac tccgtcgcgt ccgccgccat gcgccgaaag
      601 cagaaccatc gcaggggtgt cgggatgagc aacgacatgc ggcacagcca acatgccgcc
      661 gttgctgtga ccgataacga cgggcgtgcg aaagcccttt tcggccatcc actgcgcggc
      721 tatgcggttg tcctcgatgc cctcgtgcgt gagttgaaag gcggcacctt ccgcggtccg
      781 gctgtcgcgg atgctcagga tgtcgtggcc gcgccggttg aacgcgaggc atgcaaagcc
      841 catcgcggtc agcgccggcg gcaggaatct cggcgcaccg acgtagaagt tcatggtgtt
      901 gccgtggaac agcagaacag agccggtgac ggggccgtcc ggcaggtgca gtgcgccgtc
      961 gagcggcagt gtttccgtct cgatcgaaac gagctcagtt tgcatggctg cgaccagtcg
     1021 acgcgctcag accgtgttcg tggccaaatc ggcctttcat gacctcggca aacggttctt
     1081 ccgccagcgt aaccgcaccg ccgccgcgaa cccgataggc agccggatcc gcaagtgcgg
     1141 cgggtgggtg gccattcatc agcgcgcggg cggactccag gatcagccga cggaaacgga
     1201 tgacgcccat atccgtcgga ccaaggtgct ccgtcgttct atcggcgatc ggaccttagc
     1261 tgtcctgaat gcaggcgtcc tgttcggaca ccccctcgat cccggtgtag ctttcagtct
     1321 tctgtttggc ccggtcgatc agataatcat ttcccggcgg cgaatgggga tgtagccggc
     1381 gtcggtccgg gcatggatac cggacccggc ggcaaacctt tcccgttcgg cgttggtcaa
     1441 aggccgttcc ggattccagg tatagttgta gatccagcac gatgtatcgt cgatcgccac
     1501 ccaacactgg ccgtggtagt tctcgccggg acacgcgttg ggggtcaggc catggttggg
     1561 catcaggaac tggctgatgc gccaataggt ctggccagga tcgccgcggc gggcagcacc
     1621 caggaccagc ccggctgggt gatcggcaat cgtgaagcgc ggggcgccgt cgtcgcgcat
     1681 ccagcggacc gtatctgttc cggacgatac cttggtcagc agccgtgagg tcacatcatc
     1741 ggcgtcggaa acggccatat gcaggaagga gaaatgtgcg gtgtccaggc ccccctcgca
     1801 ggcctgcgcc cagttgcact gctggagctt tttcgaaacg taaatctgtg aggctggcag
     1861 catcgcgaat tcgagttccg gcaagggtgg catgtgcttg cgcgggccga gataggccca
     1921 gacgaagccg ccggcttcct ggaccggata agcgaggagg cgaacattca gttcggcgcg
     1981 gtctcgggct gatcccgggt ccatcgtcgg catcgcgagg caccttccgt cggtggcgaa
     2041 tttccagcca tgatacgcac agcgtatgcc tccgtcttcg ttgcggccaa agaacaggtc
     2101 ggccccccga tggggacagt tcggggagac caggccgacc gatccatcgg tgacgcgaaa
     2161 ggcccggaga tcctcgccca gaacagtcag gcgcaacggc ggaccattgc ggtcgggaag
     2221 ctgcgcgctg agcaggacgg gctgccagaa gcggcgaaac aaatcgccca tcggggtttt
     2281 cggaccggtt cgggtcagca gaatgttgtc ggcttccgtc aacatggtgt tccctcc
//
LOCUS       DASWQN010000078         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1855573, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000078 DASWQN010000000
VERSION     DASWQN010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855573"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="VGC82_01310"
     CDS             complement(<1..1583)
                     /locus_tag="VGC82_01310"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEY0421943.1"
                     /translation="MSDEQSQREALPEREAMEFDVVIVGGGPAGLSAAIRLKQLNPEM
                     AVCLVEKGGEIGAHILSGAVIEPRALNELIPDWKEKGAPLDTPAREDRFIFLTETRSF
                     KLPTPPQMHNHGNYIVSLGNVVRWLGQQAEELGVEIYPGFAASELLEDDGRIVGIATG
                     DMGVGKDGQPTGNFQRGMELRARYTLFAEGCRGSLSKQLMHRFNLRDGKDPQTYAIGI
                     KELWEVPAANHKPGLIEHSLGWPLDRSTYGGSFLYHFGDNLVSFGFVIGLDYSNPWLS
                     PFDEMQRFKTHPAVRQHFEGGRRISYGARALNEGGLQSIPKLSFPGGLLIGDAAGFLN
                     VPKIKGTHTAMKSGMLAAEAIAEALAGDHPAEVTGYSDRLQASWVWEELSAVRNVRPA
                     FAKFGMWGGLAYSALDTYVLRGKAPWTMHHPHADNDTLVGADTAPRIVYPKPDGVLTF
                     DRLSSVFISNTNHEEHQPAHLVLADPSKAIDINWNRFRSPEVRYCPAGVYEIVGAEEG
                     APKLQINAQNCVHCKTCDI"
     gene            1700..2506
                     /locus_tag="VGC82_01315"
     CDS             1700..2506
                     /locus_tag="VGC82_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421944.1"
                     /translation="MSANDALLLVGHGSARYPDAGNILCRQVDRLRANGLFAQIEMGM
                     LNGSPSVFDALSAIRSPLIRVVPFFMEDGYFNRIAIPRAIGAAIPGNGVVEVPWNPTA
                     TALAERAGRQRILLRPPVGTHDGMAGIIEQQALAGCAQLDVASRATAVLVIGHGSSTA
                     PGRVLALHRHTARVASTGLFARVEAACLEEAPFVADTLSMLRAHPVVVIGFFANLGGH
                     VRDDVPALIAAERVARGATGRAVLFHGSVPEDPAMTQIILDQAFGDPEGK"
BASE COUNT          448 a          818 c          783 g          520 t
ORIGIN      
        1 ttgatgtcgc aggtcttaca gtgcacgcaa ttttgcgcat tgatctggag cttcggcgcg
       61 ccctcctccg cgcccacgat ttcatacacc ccagccggac aatacctaac ctcagggctg
      121 cgaaatcggt tccagttaat gtcgatcgcc ttcgacggat ccgcgagtac cagatgcgcg
      181 ggttggtgtt cctcgtggtt ggtgttgctg atgaagaccg acgacagccg gtcgaaggtc
      241 agcaccccgt ccggcttcgg gtagacgatc cggggcgccg tatcggcccc gacaagcgta
      301 tcattgtccg cgtgcggatg atgcatcgtc cacggcgcct tgccgcgcag cacgtaggta
      361 tcgagtgcgc tgtaggccag cccgccccac atcccgaact tggcaaaggc cgggcgcaca
      421 ttgcgcacag cagacaactc ctcccatacc cagcttgcct gcagccgatc ggagtaacct
      481 gtcacttcgg ccggatgatc acccgccaga gcctcagcga tggcctccgc cgccagcatg
      541 ccggacttca tcgccgtgtg ggtgcccttg atcttcggca cgttcagaaa cccggctgca
      601 tctccgatca acaggcctcc ggggaaactg agtttgggga tcgactgcag cccgccctcg
      661 ttcagtgctc gcgcaccgta cgatatccgg cggccgccct cgaagtgttg gcgcacggct
      721 gggtgggttt tgaagcgttg catctcatcg aatggcgaca accacggatt gctgtagtcc
      781 agcccgatga caaagccgaa cgagaccaga ttatcgccaa agtggtatag gaacgacccg
      841 ccataggtgg aacggtcgag cggccaaccc aggctgtgct cgatcaaccc gggtttatgg
      901 ttggcagcgg gaacctccca cagttctttg atcccgatcg cgtaggtctg gggatccttg
      961 ccgtcccgca ggttgaagcg atgcatcaac tgtttggaca gcgaaccgcg gcagccctcg
     1021 gcgaacagcg tatagcgggc tcgcaactcc atgccgcgct gaaaattgcc ggtgggctgg
     1081 ccgtccttac cgacgcccat gtccccggtg gcgatgccga cgatgcggcc atcgtcttcc
     1141 aacaattcgg acgcggcaaa cccgggatag atctcgaccc ccagttcttc ggcctgctgc
     1201 cccagccagc gcacgacatt gccaagcgac acgatgtagt tgccgtgatt gtgcatctgc
     1261 ggcggcgtcg gcagcttgaa ggatcgggtc tcggtcaaga aaatgaagcg atcctctcgc
     1321 gccggagtat cgagcggagc acctttttcc ttccagtccg ggatcagctc gttcagcgcc
     1381 cgcggctcga tcaccgcgcc ggacaggatg tgggcgccaa tctcgccgcc cttttcaacc
     1441 aagcaaaccg ccatttcggg attaagctgt ttcagccgga tcgccgcgga caggccggcc
     1501 ggcccgccgc cgacgatcac cacgtcgaat tccattgctt cccgttcggg tagcgcttcc
     1561 cgctgggatt gctcgtcgga cattcgcggc tccttggcgt ctgctgtatc ttcgcttcag
     1621 gcttatgtgc ttcagattac ctcgcggggc aaaccggcaa tgcctcccga agatttaatc
     1681 gcttgctcag gagcttcgct tgtccgcgaa tgatgccttg ctgctggtcg gccatggttc
     1741 cgcccgttat ccggatgcgg gaaacattct gtgccggcag gtggaccggc tgcgcgcaaa
     1801 cgggctgttc gcccagatcg aaatgggcat gctgaacggc tcgccctcgg tcttcgatgc
     1861 gttgtcggcg atccgttccc cactgatccg cgtcgtgccg ttcttcatgg aggacggtta
     1921 tttcaaccgc atcgcgatcc cgcgggcgat cggtgccgcc atacccggaa atggggtcgt
     1981 cgaggttcca tggaatccca ccgcaaccgc actggcggag cgcgcgggcc gtcagcggat
     2041 tctgttgcgc cctccggtcg gtacccatga tggcatggca ggcatcatcg agcagcaggc
     2101 gttggccggc tgcgcgcagc ttgacgtcgc ttcccgcgcc acggccgtac tggtgatcgg
     2161 ccatggctct tcaaccgcgc cgggccgggt cctggcactg caccggcaca cagcgcgtgt
     2221 agcttcaact ggattgttcg ctcgggttga agcggcgtgc ctggaggaag cacccttcgt
     2281 cgccgacacg ctgagcatgc tgcgcgcgca ccccgtggtg gtgatcggct tcttcgccaa
     2341 tttgggcggc catgtccgcg atgacgttcc ggcgctaatt gcagccgaac gggtagcccg
     2401 cggagctact gggcgagcgg tgctgtttca tggttctgtg cccgaggatc cggcgatgac
     2461 gcagatcatc ctggatcagg cgtttggcga tcccgagggg aaatgacatg tttgcttacg
     2521 tcggcggcta caccacaccc gatcggaatg ggcggggaaa cgggatcaa
//
LOCUS       DASWQN010000079         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1196017, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000079 DASWQN010000000
VERSION     DASWQN010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196017"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VGC82_01320"
     CDS             complement(<1..641)
                     /locus_tag="VGC82_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0421945.1"
                     /translation="MNITVPPATLPPAAATRYPIPAPEPDMTADRLFERAVALRERLR
                     EQQDEADERGAYSPEMHQAFLQAGFYRVTQPKLFGGYEFDLGTNYRLIVEISRGHPAA
                     GWCLSLGGSHAYVVGSHWPEQAQRELFGPGGHFIAPHRPAAIGTLKPVEGGYLLNGEW
                     NYCSGIPYATHLLCGAMLEGPNAGGAAGARQVVVPRSAFEVLDDWGGDRTLGMR"
     gene            752..1732
                     /locus_tag="VGC82_01325"
     CDS             752..1732
                     /locus_tag="VGC82_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEY0421946.1"
                     /translation="MTRLTPELANRFASVALGHVTREYPNKLDHVLAGPGDVAGPRDL
                     HPVFYGSFDWHSCVHGYWMLARLYRRFPAMPAAPAIRTLFDSHLVDGLIGRECDYLER
                     PGSAGFERPYGWAWLLKLAAELGLHQTAEGQAWSAALSPLADLFAARFMRFLPLATYP
                     VRAGVHSNTAFAVRLAVDYQDAALRSLLSDTARRWYANDADCQAWEPGGDEFLSPALI
                     EAECMRAALPDAEFRPWFARFLPRLGQLQPETLFTPATVSDRSDGKIAHLDGLNLSRA
                     WCWKALADFSPDPEAVRRTAEAHIDASLPHVSGEYMGEHWLASFAVLALE"
     gene            complement(1780..>2407)
                     /gene="trpA"
                     /locus_tag="VGC82_01330"
     CDS             complement(1780..>2407)
                     /gene="trpA"
                     /locus_tag="VGC82_01330"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY0421947.1"
                     /translation="IQAAGKRGLAAGVKVGNVLTMVRDFRMEDAATPVVLMGYLNPIL
                     SYGPERFCSDAAAAGVDGLIIVDLPTEEADLLLPHSNASGLDFIRLIAPTTDDERLPH
                     VLNGSSGFVYYVSITGITGTSSATAEHLEAAIPRLRKATPLPIAIGFGVRTPEQAATA
                     SRVADAAVVASALIDTLSANLDADGRPLPGAVGKVLDQVRALAAAVRR"
BASE COUNT          402 a          756 c          828 g          421 t
ORIGIN      
        1 cgcatcccca gggtgcggtc gccgccccag tcgtccagca cctcgaacgc cgaacgcggc
       61 acgacgacct ggcgagcgcc cgccgctccg cccgcgtttg gcccttccag catcgcgccg
      121 caaagcagat gcgtggcata ggggatgccg gagcagtagt tccattctcc gttcaggagg
      181 tagccgccct cgactggctt cagcgtgcca atggcggctg gacggtgcgg ggcgatgaag
      241 tggccgccgg gaccgaacaa ttcacgttgc gcctgttccg gccagtgcga tccgacgacg
      301 taggcatgcg atccaccaag cgaaaggcac cagccggctg cggggtgacc acgggaaatc
      361 tcgacgatca ggcggtagtt ggtcccgagg tcgaactcgt acccgccaaa cagcttcggt
      421 tgcgtcacgc ggtagaagcc ggcctgcagg aaggcctggt gcatctcggg cgagtaggca
      481 ccgcgttcgt cggcctcgtc ctgttgttcg cgcaaccgct cgcgcagtgc gacggcccgt
      541 tcgaacagcc gatcggcggt catgtcgggt tcgggagccg gaatcggata acgtgtcgcg
      601 gcggcgggcg gcagcgtggc gggcggcact gttatattca cgtaaggcac tccaggatgg
      661 tcgggcaaat gtgcggccgg ttcgccgatc tcgcaatcgc ggcgatttca tccaccaggc
      721 gttccgcccg gcgcaacccc ggaggcagtc gatgacgcga cttacgcctg aattggcaaa
      781 ccgcttcgcc agcgtcgcac tggggcacgt cacgcgcgaa tatccgaaca aactggatca
      841 tgtgctggct ggacctggcg atgtcgcagg gccgcgagac ttgcatccgg tgttctatgg
      901 cagcttcgac tggcactcct gcgtgcacgg gtactggatg ctggcgcggc tttatcggcg
      961 ctttcccgcc atgccggcgg ccccggcaat ccgaaccctg ttcgactccc acctggtcga
     1021 tggcctgatc gggcgggagt gcgactacct cgagcggccc ggttcggccg ggttcgagcg
     1081 gccctacggg tgggcgtggc tgctgaagtt ggccgcggaa ctggggttgc atcagacggc
     1141 ggagggccag gcctggtccg cggcgctgtc cccattggct gacctgttcg ccgctcggtt
     1201 catgcggttt ctgccgttgg cgacctatcc ggttcgggcc ggagtccatt ccaataccgc
     1261 cttcgcggtg cggctggcgg tggattatca ggatgccgca ttgcgcagcc tgctgtcgga
     1321 taccgcccgg cggtggtacg cgaacgacgc ggattgccaa gcctgggagc cgggcggcga
     1381 tgagttcctg tccccggcgt tgatcgaggc ggagtgcatg agggcggccc tgcctgacgc
     1441 cgagttccgg ccgtggttcg cgaggttctt gccccggttg ggtcagcttc agccggaaac
     1501 gttgttcacc cccgccacgg tcagcgatcg gagcgacggc aaaatcgcgc atttggacgg
     1561 attgaacctg agccgggcgt ggtgctggaa agcgttggcg gatttctcgc cggacccgga
     1621 ggcggttcgg cggaccgccg aagcgcatat cgatgccagc ctgccgcatg tgagcgggga
     1681 atacatgggc gagcactggc tggccagctt cgcggtgctg gcgctggaat gagctgaaac
     1741 aatacggcaa aggagaggcg gcagaggatg gaagaatggt taacgccgca cggcagcggc
     1801 caacgcccgc acctgatcga gtactttccc aactgctcca ggcagcggcc gcccatccgc
     1861 gtcaagattt gccgaaagcg tgtcgatcag cgccgaggcc accaccgcag catcggcgac
     1921 gcgggatgca gtcgcggcct gttcaggcgt gcgcacccca aagccgatcg cgatcggcag
     1981 gggggtggct ttgcgcagcc gggggatcgc cgcttccagg tgttcagcgg tcgcgctgct
     2041 tgtgccggta atgccggtga tgctgacgta atagacgaaa ccggaactgc cattcagcac
     2101 gtgcggcagg cgttcgtcgt ccgttgtggg cgcgatcagg cggataaaat ccaagccgct
     2161 ggcattgctg tggggcagca gcaggtcggc ttcctcggtc ggcagatcga cgatgatcag
     2221 cccgtccacg ccggcggcag cggcatcgct gcaaaaccgt tcagggccgt acgacaggat
     2281 cgggttgagg tagcccatca gcaccaccgg cgtggctgca tcctccatcc gaaagtcacg
     2341 caccatagtc agcacattgc caaccttgac tccggcggcc aagccccgct tgcctgcggc
     2401 ctggatg
//
LOCUS       DASWQN010000080         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_800625, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000080 DASWQN010000000
VERSION     DASWQN010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800625"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VGC82_01335"
     CDS             <1..438
                     /locus_tag="VGC82_01335"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0421948.1"
                     /translation="TEIAHPILSPNGCGLSPDGKTLYAADTEGARLWAFDIEAPGVLR
                     QPAPYAHHSGRVIAGLGGNARFDSLAVLANGNIAVATLNTGYITEISPNGDIVQAVKM
                     PDTYPTNICFGGPDMRTAYITLSDSGRLGVMQWSSPGLKLNYG"
     gene            complement(529..>1563)
                     /locus_tag="VGC82_01340"
     CDS             complement(529..>1563)
                     /locus_tag="VGC82_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0421949.1"
                     /translation="RAALTLSVTLMCAGSFLIAIAPTYAVIGLGAPILLLVARLIQGI
                     SLGGEYGTSATYLSEIAVSKHRGFYSSFQYVTLIGGQVLGSLTLLAMQQSLTAHELEA
                     WGWRIPFAIGAALSVFGLYLRRNLEETDAFKKAAGKPRGSPIRELLKYPSQILLVFGL
                     TMGGTTAFYTYTTYMPTFLVNTVKLTRDQATQISFITLVIYAALQPVFGAISDKVGRR
                     PVLMCFGILGTLCTIPILTTLETTRDYMTALGLIMAALMIVSGYTSINAVVKAELFPA
                     GVRALGVGLPYAVAASIFGGTAPYVALAFKDNGHEAWFYYYVTVLIFGSLLVYTFMGD
                     TKRLSQIDRD"
BASE COUNT          323 a          463 c          511 g          266 t
ORIGIN      
        1 acggaaatcg ctcaccccat actcagcccg aatggctgcg gcctgtcgcc ggacggcaag
       61 actctatatg ccgccgacac cgagggcgct cggctgtggg ccttcgacat cgaggccccg
      121 ggcgttctgc gccagcccgc accctatgca catcacagcg gccgggttat cgccgggctt
      181 ggcggcaatg cccggttcga cagcctggcg gtactggcga acggaaatat cgcggttgcc
      241 acgttgaaca ccggctacat cacggagatt tcgccgaacg gcgacatcgt gcaggcggtg
      301 aagatgccgg acacctatcc gaccaatatc tgctttggtg ggccggatat gcggacggcc
      361 tacattacct tgtcggacag cggccggctt ggggtgatgc agtggtcatc gccgggactg
      421 aagctgaact acggctgaac ccgaagccgg ccctttggcg cgactgcgaa aatgcacttc
      481 cgtccgcagg cgcctggagt gtccccgtct tgcgatcgaa gcggcgcttc agtcgcggtc
      541 gatctgcgaa agtctcttcg tatcgcccat gaacgtgtaa accagcagtg acccgaagat
      601 cagcaccgtc acatagtaat agaaccaggc ctcgtgcccg ttgtccttga acgccaacgc
      661 gacatagggc gcggtgccgc cgaaaatgga tgccgccacc gcatagggca gccccacgcc
      721 aagggcacgc acgccggcgg gaaacagctc ggctttgacc acggcgttga tcgaggtgta
      781 gccggacacg atcatcagcg ccgccatgat caggcccagg gcggtcatgt agtctcgagt
      841 ggtctccagg gtggtgagga tcgggatggt gcaaagcgtg ccgaggatgc cgaagcacat
      901 caacaccggg cggcggccga ccttgtcgga aattgcaccg aacacgggtt gcagcgcggc
      961 atagatcacc agggtgatga aggagatttg cgtcgcctga tcgcgcgtga gcttgaccgt
     1021 gttgaccagg aaggtcggca tataggtggt gtaggtatag aaagccgtgg tgccgcccat
     1081 cgtcaggccg aacaccagca ggatctggct gggatattta agcagctccc tgatcgggct
     1141 gccgcggggc ttgccggcgg cttttttgaa agcgtcggtt tcctccaggt tccggcgcag
     1201 atagaggccg aacactgaga gggcagcgcc gatggcgaat gggatgcgcc agccccaggc
     1261 ctccaactca tgggctgtaa ggctttgctg catcgccagc agggtcaggc tgccgagtac
     1321 ctggccgccg atgagggtga cgtactggaa cgaactgtaa aagccccggt gcttcgagac
     1381 ggcgatctcg ctgagatagg tggccgaggt accgtattcg ccgcccagcg atatgccctg
     1441 gatcaagcgg gcgaccagca acaggatagg cgcgccgagg ccgatcaccg cataagtcgg
     1501 ggcgatggcg atcaggaaag aaccagcgca catcaaagtc accgacaggg tcagcgcggc
     1561 acg
//
LOCUS       DASWQN010000081         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1196452, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000081 DASWQN010000000
VERSION     DASWQN010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196452"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1045
                     /gene="hrcA"
                     /locus_tag="VGC82_01345"
     CDS             11..1045
                     /gene="hrcA"
                     /locus_tag="VGC82_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY0421950.1"
                     /translation="MPPGLDARSAAVLREIVEQYVATGEPVGSRTLARRLPMSLSPAT
                     IRNVMADLTDAGLLFAPHTSAGRLPTDQGLRLFIDGLLNFGELNEDERESISAALAAS
                     GRSLEDTLAEASSLLSGLSAAAGLVLAPKSEGAVKHIEFVPLGPGRALVVLVNSDGQV
                     ENRVIETPPGLPPSALQQASNYLNARLSGLPLAELRRVVGEEIAANRTELDALSTLVV
                     SAGLATWTGEGRSGSLIVRGQARLLSDVTQLDRITAIQALFERLEAQETMLRLLELAE
                     QSDGVRIFIGAESGVFNTSGVSMVVAPARNEAGRIVGAIGVIGPSRINYGRIIPVVDY
                     TARVIGQLLG"
     gene            1226..1819
                     /locus_tag="VGC82_01350"
     CDS             1226..1819
                     /locus_tag="VGC82_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003623820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY0421951.1"
                     /translation="MSEQTAAAPDAAPQQGDHQSELTPDQALQAVTDRIVALEAELAE
                     MKERWMRAEAENANVRSRARKDIDDTRQYAVQKFAADVVEAAENLKRGIDSLPPASND
                     QPAIVAKVREGLEGMERSFVGTLERNGIKQEDPVGQPFDPNLHQAMAEQESETHAPGT
                     VVQAWSKAWTLNGRLLRPAMVVVAKAREAELSGPVKG"
     gene            1998..>3299
                     /gene="dnaK"
                     /locus_tag="VGC82_01355"
     CDS             1998..>3299
                     /gene="dnaK"
                     /locus_tag="VGC82_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY0421952.1"
                     /translation="MSKVIGIDLGTTNSCVAILEGKDVRVIENAEGARTTPSMVAFAE
                     GGERLVGQSAKRQAVTNPTNTLYAIKRLIGRRYDDPLVAKDKAMVPYAIVRGDNGDAV
                     VESRGGKYSPSQVSAFILGKMKETAEAYLGEPVSQAVITVPAYFNDSQRQATKDAGRI
                     AGLDVLRIINEPTAAALAYGMDKKSAGTIAVYDLGGGTFDISVLEIGDGVFEVKSTNG
                     DTFLGGEDFDLRVIDYLADEFRKDQGIDLRKDKLALQRLKEAAEKAKIELSSSKETEI
                     NLPFITADASGPKHLVMKLSRAKLESLVDDLISRTMDPCRAALKDAGVTANEIQEVIL
                     VGGMTRMPKVIEAVKAFFGKEPARNVNPDEVVAIGAAVQGGVLKGDVKDVLLLDVTPL
                     SLGIETLGGVFTRLIDRNTTIPTKKSQTFSTADDNQQAVTIK"
BASE COUNT          656 a         1037 c         1087 g          519 t
ORIGIN      
        1 gccgccaccg ctgccgcccg gcctggatgc gcgctccgcc gcggtgctcc gcgagatcgt
       61 cgaacaatac gtcgccaccg gcgaaccggt cgggtcccgt accctggcgc gccggttgcc
      121 catgagcctg tcgcccgcga caatccgcaa cgtcatggcc gacctgacag atgccgggct
      181 gctgttcgcc ccgcacacct ccgccgggcg gctgccgacc gaccaggggc tccgcctgtt
      241 catcgacggg ctgctgaatt tcggcgaact gaatgaagat gaacgtgaat cgatctccgc
      301 ggcgctcgcc gcttccggcc gcagcctgga agacacgctg gccgaggcca gttcgctgct
      361 ttcaggtctg tccgccgccg ccgggctggt gcttgcccca aagtcggaag gggcggtgaa
      421 acacatcgag ttcgtcccgc ttggaccggg tcgagcgctg gtggtgctgg ttaacagcga
      481 tgggcaggtg gaaaaccggg tgatcgagac accgccgggg ctgccgccgt cggccttgca
      541 acaggccagc aactacctga acgccaggct gtccggcctg ccgctggccg aacttcgccg
      601 tgtggtgggg gaggaaatag cggcgaaccg gaccgaactc gatgccctct cgaccctggt
      661 cgtctctgcc gggctggcca cctggactgg tgaaggccgc tccggcagcc tgatcgtccg
      721 cgggcaggca cgattgctct ccgacgtgac gcaattggat cgcatcaccg cgattcaagc
      781 gctgttcgag cggctggagg cgcaggagac aatgctgcga ctgctcgaac tggcggaaca
      841 atccgacggc gtgcggattt tcatcggcgc ggaaagcggt gtgttcaaca cctccggcgt
      901 gtcgatggtg gtggcgccgg cgcgaaacga ggccggacgt atcgtcggcg cgatcggggt
      961 gatcggtcca agccggatca actacggccg gatcatcccg gtcgtggact ataccgctcg
     1021 ggtaatcggg caattgctgg gttgagcccc aaggcgggac cacgtccaag cgggaccaca
     1081 tccaagcggg accacaccca gggcgggcca cagcgaaaca aacgagtcga atcaggttgt
     1141 tctcccctgc catggtgcct aatttaaggc cgcaaccgac gcgatgcgcg aaatgaccat
     1201 tgaacctcag cccattcagg ataccatgtc agaacaaacc gcggccgcgc cagacgccgc
     1261 gccccaacaa ggcgaccatc aaagcgagct aacgccggac caggcgctgc aagcggtgac
     1321 cgaccggatc gtcgcgctag aggccgaatt ggcggaaatg aaggaacgct ggatgcgggc
     1381 cgaggcggag aacgccaacg ttcgcagccg tgcccgcaag gatatcgacg atactcgcca
     1441 gtacgcggtg cagaaatttg cggccgatgt ggtggaagcg gcggagaacc tgaagcgcgg
     1501 gatcgacagc ctgccgcccg cctcgaacga tcagccggcg atcgtcgcca aggtccggga
     1561 gggcctggaa gggatggaac gcagcttcgt cggcacgctg gagcggaacg ggatcaagca
     1621 ggaagacccg gtcggtcagc cgttcgaccc caatctccac caggccatgg cggagcagga
     1681 aagcgagacc cacgcccccg ggaccgttgt ccaggcgtgg tccaaggcct ggacgctcaa
     1741 tggccggctg ctgcgcccgg cgatggtcgt cgtcgcgaaa gcccgggaag cggaactcag
     1801 cggtccggtc aaggggtagc ctgatccagc atggtgacgc ccattgtgat cccatggccc
     1861 aatattgtga cgctgcggaa cgaaaatttt agcttttacc cttgtcccgg cggcgtcagt
     1921 ctcatacatc cagaccattc acaaaaggga cagcgccggt gcttcgggcc gccgccgtcc
     1981 gctgaaagga gctttcgatg agtaaagtga tcggtatcga tctcggtacc accaattcct
     2041 gtgtcgccat cctggaaggc aaggacgtcc gcgtcatcga gaacgccgag ggcgcgcgca
     2101 cgactcccag catggtggcc tttgccgagg gcggcgagcg gctggtcggc cagtcggcca
     2161 agcgccaggc ggtcaccaac ccgaccaaca ctctgtatgc gatcaagcgc ctgattgggc
     2221 gccgctacga cgatccgctg gtcgccaagg acaaggcgat ggttccgtat gccatcgtcc
     2281 gcggcgacaa tggcgatgcc gtcgtcgaaa gccgtggtgg caaatattcg cccagccagg
     2341 tcagcgcgtt catcctcggc aagatgaagg agacggccga agcttacctc ggtgagccgg
     2401 tgtcccaggc ggtgatcacc gtcccggcct atttcaacga cagccagcgc caggcgacca
     2461 aggatgccgg ccgcatcgcc ggacttgacg tgctgcgcat catcaacgaa ccgacggcgg
     2521 ccgcgctggc ctacggcatg gacaagaagt ccgccggcac catcgcggtg tacgaccttg
     2581 gcggcggcac cttcgacatc tccgtgctgg aaatcggcga cggcgtgttc gaagtgaagt
     2641 ccaccaacgg cgacacgttc ctgggcggcg aggatttcga cctgcgcgtc atcgactatc
     2701 tggccgatga attccgcaag gaccagggca tcgacctgcg caaggacaag ctggcgctgc
     2761 agcggctgaa ggaagccgcc gagaaggcca agatcgagct gtcctcgtcg aaagagaccg
     2821 agatcaacct gccgttcatc accgcggatg cctctgggcc gaagcacctg gtgatgaagc
     2881 tgtcccgcgc gaagctggaa tcattggtcg atgacctgat cagccgcacg atggacccgt
     2941 gcagggcagc gttgaaggac gccggcgtca ccgccaatga gatccaggaa gtaatcctgg
     3001 tcggcggcat gacccggatg ccgaaagtca tcgaggcggt gaaggcgttc ttcggcaagg
     3061 agccggctcg caacgtcaac ccggacgagg ttgtcgcgat tggtgcggcg gtgcagggcg
     3121 gcgtgctcaa gggcgacgtc aaggacgtgc tgctgctgga cgtgacgccg ctgtcgctgg
     3181 ggattgagac gctgggtggt gtgttcaccc ggttgatcga ccgcaatacg acgatcccga
     3241 cgaaaaagag ccagaccttc tccaccgccg acgacaacca gcaggcggtg accatcaag
//
LOCUS       DASWQN010000082         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1592641, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000082 DASWQN010000000
VERSION     DASWQN010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592641"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VGC82_01360"
     CDS             <1..873
                     /locus_tag="VGC82_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421953.1"
                     /translation="PRNIADMWSGVWADGVDGALCPDGMQVLFFRGTDYLVYDLAADA
                     VTAGPLPVSGIALDPRPAGMVRPARELSLVQANGIVGYLMDKGQMSLKSGTTPPAPPD
                     HLVISPAALAGVRFAGVGATSADFIDNVDQRMAVALWRLARWLNASSENVTTIRHMGI
                     GHGNGSDDDCHNTGRAIDFAGVDGDVDGVAFAVQVQRDWGSKPSSDPAAYRLSDADQP
                     ACSLFKRAYTFGAFECESRGRGKEAWPPLEIGEAGYVIYPDYYGTGTPDNLRLRAAHS
                     NHIHMQVGPTHGPY"
     gene            1037..2395
                     /locus_tag="VGC82_01365"
     CDS             1037..2395
                     /locus_tag="VGC82_01365"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0421954.1"
                     /translation="MSLPPIVASILQSGAPTLLAAIALPPPFNLIASAVVSGVLGQFL
                     PEGDAPAQSKPGGGAALSPAQVTQVVEAHAKDPDFLPKLREAEAKLKEYEAANDLKFA
                     ELQVEDKNRAGRFQIATGTAAALLRWGMGIVVVAILAMAAVVAGSIMLVSGGWELRPD
                     KAQLAVGVFGLIGTVVGYISGYGAQIIGFYYGSSQGSKEKSESLDAVLQQQGQQLGRA
                     AAAATTAAATSAATTATTVSAAAASAAQTAVATIAARAGGSAAEPVQWKQGPFGGVRW
                     RLTQAGILVEGETAPARTVGEPATVRRIWRDFGDIIKGVCAPLGVPAEVVVTTIAVES
                     RGVIAATLHEPDGRTSVGLMQTLTETASQVMGRNITAADLEKPEISIEAGTRYIASQR
                     AKTQYDPILVAAAYNAGGIDPPRPGDDNPWKLRCTGDHLTRTKLYYNDAVAVAKEDGW
                     FK"
BASE COUNT          504 a          864 c          886 g          415 t
ORIGIN      
        1 ccgcgcaaca tcgccgacat gtggagcggc gtctgggccg atggcgtcga tggggcgctt
       61 tgtcccgatg ggatgcaggt actgttcttc cggggcacgg actacctggt ttacgacctc
      121 gcggccgatg ccgttaccgc cgggccgctg ccggtcagcg ggatcgccct ggatccgcgt
      181 cccgccggga tggtccggcc cgctcgggag cttagcctcg ttcaggccaa cggcatcgtc
      241 ggatacctga tggacaaggg gcaaatgagc ctgaagtccg gcaccacccc gccggcaccg
      301 ccggatcatc tggtgatctc gcctgccgcg cttgccggcg ttcgcttcgc cggcgtcggc
      361 gccacgtcgg ccgacttcat cgataatgtt gatcagcgca tggccgtggc gctttggcgc
      421 ctcgcccgct ggctcaacgc ttcgtcggag aacgtgacga ccatccggca catgggcatc
      481 ggccacggca acggatccga cgacgattgc cacaacaccg gccgcgccat cgacttcgcc
      541 ggtgtggacg gcgacgtgga cggggtggcc ttcgccgtcc aggtgcagcg cgactggggg
      601 tcaaaacctt cgagcgaccc ggcggcctac cggttgtcgg acgccgacca accggcctgt
      661 tcgctgttca agcgcgccta caccttcggc gccttcgaat gcgagagtcg aggccgcgga
      721 aaggaggcat ggccgccgct ggagatcggc gaggccggct atgtcatcta cccggactac
      781 tacggcaccg gaacgcccga caacctgcgg ctgcgggcgg cccattcgaa ccacatccat
      841 atgcaggtgg gcccgaccca cggaccgtac tgaaccgcgc cactgagccg cgccgggcga
      901 ttggctcgcc cggacccccg gcccgatgtc tgcgggacac gaagcgtccg aacaatacac
      961 tgctttgcct gtgattactt gctgcccacc gatgcggttc ccgcgcggcg gcacggaacg
     1021 aagaggaagc cctgaaatgt cgttacctcc aatcgtcgcc tccattctgc agtcgggtgc
     1081 gccgacgttg ctggccgcga tcgcattgcc gcctccgttc aacctgatcg cgagcgcggt
     1141 ggtcagtggc gtgcttggcc agttcctgcc cgaaggcgac gccccggcgc agagcaagcc
     1201 aggcggcggc gcggcgttgt cgccggccca ggtcacgcag gtcgtcgaag cgcatgcaaa
     1261 ggacccggac ttcttgccga agctgcggga agccgaagcg aaactgaagg aatacgaagc
     1321 cgcgaacgac ctgaaattcg ctgagcttca ggtcgaagac aaaaaccggg caggccggtt
     1381 ccagatcgcc acgggcaccg ccgccgcgct cctgcgatgg ggaatgggca tcgtcgtcgt
     1441 cgcgatcctg gcaatggccg ccgtcgtggc cggcagcatc atgctcgtca gcggcggctg
     1501 ggagcttcgg ccggacaagg cgcaactcgc ggtcggcgtc ttcggtctga tcggcacggt
     1561 tgtcggatac atcagtggct acggcgccca gatcatcggc ttttactatg gctccagcca
     1621 gggctcgaaa gaaaaaagcg aatcgctcga cgctgtactg cagcagcagg gccagcagct
     1681 cggacgggcc gccgcggcgg cgacgacagc cgcggccact tcagccgcga cgaccgcgac
     1741 cacggtgtcc gcggccgctg cctcggctgc ccaaacagcc gttgcgacga tcgccgcccg
     1801 agccggtgga agtgcggctg aaccggtgca gtggaaacag ggaccgttcg gcggcgtgcg
     1861 ctggcgtctg acccaagccg gcatcctggt ggaaggtgaa accgctccgg cgaggaccgt
     1921 tggcgaaccg gcgaccgtgc gaaggatctg gcgcgatttc ggggacatca tcaaaggtgt
     1981 atgcgccccg ctgggcgttc cagccgaggt cgtcgtgacc acgatcgcgg tggaatccag
     2041 aggcgtcata gcggcgactc tgcacgagcc tgacgggcgg acatccgtcg gcttgatgca
     2101 gacgttgacc gagacggctt cccaggtgat ggggcggaac atcaccgccg ccgacctgga
     2161 gaagccggag atatcgatcg aagccgggac ccggtacatc gcttcgcagc gagcgaagac
     2221 gcagtacgac ccgatcctgg tcgcggcggc ctataatgcc ggaggcatcg atccgccgcg
     2281 cccaggggac gacaatccgt ggaagctgcg atgcaccggc gatcacctga ccagaacgaa
     2341 actctactac aacgacgcgg tggcggtagc caaggaggat ggctggttca agtaataccg
     2401 acccgccgag ggattgacgc atcggtagcg gctcccgccg tcatggtggt caaacgcacg
     2461 cggaaggcat gatgccgacc ttcatcggca cgacgaggtc atactgccgg aggttcgata
     2521 ccaatgcaga agcagtacag gaaacgcccg attgcatggc gataccaatt gaagaagcac
     2581 cggccaacta cgggccatac cgcccaaaaa ttcataccag ataagtaatg ggggagagaa
     2641 agctgcagcc agcgccgcat gggcctggt
//
LOCUS       DASWQN010000083         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_668819, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000083 DASWQN010000000
VERSION     DASWQN010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668819"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1030
                     /gene="argF"
                     /locus_tag="VGC82_01370"
     CDS             137..1030
                     /gene="argF"
                     /locus_tag="VGC82_01370"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY0421955.1"
                     /translation="MDLRDFDSATLRQMLDVASGFKRAGGVSSRPLAGKTLALIFEKP
                     STRTRVSFEVGMRQLGGDVVMLSGEGTQLGRGESVADTARVLSRYVDAIMLRTDKASK
                     LHELAQYATVPVINGLTEASHPCQLMADVLTFEEHRGPIAGQVVAWCGDGNNVARSWI
                     EAAVRFGFTLRLATPDGLRPPEDLVAWARAQGGRIELTDDPGAAVAGARCVVADTWVS
                     MSDDPNESRHNLLAPYRVTSRLMAQAAPDALFMHCLPAHRGEEVDADVIDGPQSVVFD
                     EAENRLHAQKGVLAWVMGAAR"
     gene            1239..1787
                     /locus_tag="VGC82_01375"
     CDS             1239..1787
                     /locus_tag="VGC82_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0421956.1"
                     /translation="MSAPPIVIIAEPDPRISSILRVEFSHWDFAVFLASSGLEAEEYA
                     AHAVAHLIVLDTKLHLGAYDACARIRRRAGYATKPIVLTANEISPTLKMVAAKVGATV
                     LLPKPYSVAGLFGAIRPFLSSNDLLLTHRARGPGMSQAREWMPAPPPNMRSGGDSALT
                     RNAQLLPIVRSQGVAIPLVRNR"
BASE COUNT          347 a          680 c          725 g          397 t
ORIGIN      
        1 ccatgctgcg cgctgcggcg acgaaggtgg cggctgcggt ggcgagcaag gccgcggcac
       61 agtgaccggg gccgccgttc gcaggtcgga cgtccgcggc gagtcctcgg agaataaggc
      121 cgccccgcgg catttcctgg atctgcgtga cttcgattcg gcgacgctgc ggcagatgct
      181 ggatgtcgct tcgggcttca agcgggcagg gggcgtgtcg tcgcgccccc tcgccggcaa
      241 gaccctggcg ctgatcttcg agaagccatc gacgcggact cgcgtgtcgt tcgaggtcgg
      301 catgcggcaa cttggtggcg acgtcgtcat gttgtccggc gaaggcacgc agcttggtcg
      361 cggcgagagc gtggccgaca ccgcacgcgt gctgtcccgc tatgtcgatg cgatcatgct
      421 gcggacggac aaggcctcga agctgcatga actggcgcaa tacgcgaccg tgccggtgat
      481 caacggcctg accgaggcgt ctcatccatg ccagttgatg gccgacgtgc tgacgttcga
      541 ggagcatcgc ggcccgatcg cgggccaggt cgtggcgtgg tgcggcgacg gcaacaacgt
      601 cgcacggagc tggatcgagg cggcggtgcg gttcggattc acgttgcgcc tggcgacccc
      661 ggacgggctg cggccgccgg aggatctggt ggcctgggca cgcgcccagg gcggccggat
      721 cgagctgact gacgaccctg gcgcggcggt agcgggggca cggtgcgtcg tggccgacac
      781 ctgggtatcg atgtcggatg atccgaacga gagccggcac aatctgctgg cgccgtaccg
      841 ggtgaccagc cggctgatgg cacaggccgc ccccgacgcg ctgttcatgc actgcctgcc
      901 ggctcatcgc ggggaggaag tggacgccga cgtgatcgac gggccgcagt cggtggtgtt
      961 cgatgaggcg gagaaccggc tgcatgctca gaagggtgtt ctggcgtggg taatgggcgc
     1021 ggcgcggtag ggcgttccag cctgacttta caggccgaaa aatgccctac cttttggttt
     1081 gatcgcatga ttcatacgct ttggacgttc cgcccagcgt gatcatgctt tggacgacgc
     1141 cggtatcctg tcgaagacat cctcggacgc tgcgatcgta cacccagggc ctttgcgcct
     1201 taacgcttcg tcaacctttt gccggcatgt tgcttcgcat gtcagcccct cccatcgtca
     1261 tcatcgccga accggacccc cgcatcagca gcatcctgcg ggtcgagttc agtcactggg
     1321 atttcgctgt gtttcttgct tcctccgggt tggaggcgga ggaatacgcc gcccatgccg
     1381 ttgctcatct gatcgttctc gacaccaagc tgcacctggg ggcctatgac gcctgcgcac
     1441 gtatccgccg ccgggcaggg tatgccacca agccgatcgt gctgaccgcg aacgagatat
     1501 cacccacgct caagatggtt gccgccaagg tgggtgcgac agtcctgttg ccgaagccct
     1561 attcggttgc cggtctgttt ggcgcgatca ggccttttct ctcgagcaac gacctgctgc
     1621 tgacgcatcg cgcgcgcggg cctggcatga gccaagccag ggaatggatg ccggcgccac
     1681 cgccgaacat gcgatccggc ggcgactcgg ctttgacgcg gaacgcgcag ttgttgccga
     1741 ttgtccgtag ccagggtgtg gcgatcccgc tggttcggaa tcggtagttc ggagacacgt
     1801 ggctcttgcc gacctgctca agcgcgattc cggcttcagc agccagcgat gccgccgcct
     1861 tctgcagacc acaaccgggc acaccacaac cgaatcgtag catgacagca ccccggcgat
     1921 ccggtcgtcg gaccgctctg tctgcgcgcc tgccatagca ccctcgacca tggtggcatt
     1981 ggatgacgat gctacagcct gtttggttcc ggctcgtccg gctcaggagt aggggtagca
     2041 gcacgcgaac cgcctggatc gcaggatgac accattggcg tcgaactccg gcaggtggcg
     2101 ccatcgcgtg accatccgat aaaccgccgc cgccaactcg gtcgtccgc
//
LOCUS       DASWQN010000084         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1988547, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000084 DASWQN010000000
VERSION     DASWQN010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988547"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1534
                     /locus_tag="VGC82_01380"
     CDS             <1..>1534
                     /locus_tag="VGC82_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421957.1"
                     /translation="RVALDGTVTVLGWAVCAVGIANIGIYIDGVKVGDAQLALPRPDV
                     AGEFPNNPMAHLSGFQCKARIPELAGGTHELLIVVRNGMDDVLEETDTITFNPPVEPR
                     SALSAEPSEFRFELDNPRVVDGVMAEPVTGRLTIEGWVVAQSGISHIEVRLDGQRLGQ
                     AHYGLARQDVGIAFPEMPNSLRSGYAFHCPPRSLRSGIHVVTLIVHAKNGTELVHRFD
                     IEVKRPDGEDELVSIRRRITHVEATVLAGVLEDLRFHPRFRLVLRQTGTATLAQLRAT
                     VASLRTQIIPDWHLTILPADGQTADGVREVLAECADDLLERIAIADDADMAFADGEDA
                     SDETATFYGALCPGDELGCDALAEFAVAAGLHREADFLYADETRFSAASRLREPFFKP
                     DFSPDLLLSTNYIGRPWFAAADLLRRCGISSSTLFSSGEYDLVLRLTENARSVHHVPK
                     LLAKRGPEPRDDEEACHAALAEAANRLGFDAEVLNGCLPGTFRLKRTQRATGKVSIII
                     PT"
BASE COUNT          269 a          513 c          485 g          267 t
ORIGIN      
        1 cccgcgtggc cctggacggc acggtcaccg tgctcgggtg ggcggtgtgc gcggtcggca
       61 tcgccaacat cggtatctac atcgacggcg tgaaagtcgg cgacgcccag ttggccctgc
      121 caaggcccga tgtcgccggt gaattcccaa acaatcccat ggcgcacctt tccggcttcc
      181 aatgcaaggc gcggatcccc gagctcgccg ggggaaccca cgagctactg atcgtggtgc
      241 gcaacggcat ggacgatgtc ctggaagaaa ccgacacgat caccttcaac cctccggtcg
      301 aaccacgatc cgcgctatcc gcggaaccgt ccgagtttcg cttcgaactc gataacccgc
      361 gcgtggttga cggcgtcatg gcggaaccgg tgaccggccg tctgaccatc gagggctggg
      421 tggtggcgca atccggaatc agccacatcg aggtgcggct ggacggccaa cgcctcggtc
      481 aggcgcatta tgggctggca cgccaggacg tcggcatcgc cttcccggaa atgcccaatt
      541 cgcttcgcag cggctatgcg ttccattgcc caccccgatc gctacgttcg ggaatccatg
      601 tcgtaacgct gatcgtgcat gcaaagaacg gcacggaact ggtccaccgc ttcgacatcg
      661 aggtcaagcg ccccgacggc gaggatgagc tcgtcagcat acgccggcgc atcacgcatg
      721 tggaagcgac cgtgctggcg ggcgtgctgg aggatctgcg gttccatccc cggttccgcc
      781 tggtgctgcg acagaccggg accgcgacgc tcgcccagtt gcgcgctacc gtggcgtcgc
      841 tgcgcactca gatcataccg gactggcacc tgacgatcct gccggcggac gggcaaaccg
      901 cggacggggt gcgcgaggtt ctcgcggaat gtgccgacga cctgctggaa cgcattgcga
      961 tcgccgacga tgcggacatg gcgtttgccg acggggaaga cgcgtccgac gaaaccgcga
     1021 cgttttatgg cgcgctgtgt ccgggcgacg aactgggctg cgatgcgctg gccgagttcg
     1081 ccgtggccgc gggactgcac cgggaggccg atttccttta tgctgacgaa acccggttca
     1141 gcgccgccag ccgcctgcgt gaaccgttct tcaagcctga tttctcgccc gaccttctgc
     1201 tttccaccaa ttatatcggc cgcccctggt tcgcggccgc cgacctgctg cggcgctgcg
     1261 gcatttcctc aagcacgctg ttttcgagcg gcgagtacga tctggtcctg cggctgacgg
     1321 agaacgccag gtcagttcat catgtgccga agctgctggc aaagcgcggc cccgaaccac
     1381 gggacgacga agaagcatgc cacgccgcgc ttgccgaagc cgccaatcgc ctcggcttcg
     1441 atgcggaagt cctgaacggc tgcctgcccg gcactttccg gctgaaacgc acgcagcgcg
     1501 ccaccggcaa ggtctccatc attatcccga cctg
//
LOCUS       DASWQN010000085         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_669174, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000085 DASWQN010000000
VERSION     DASWQN010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669174"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VGC82_01385"
     CDS             <1..1341
                     /locus_tag="VGC82_01385"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0421958.1"
                     /translation="ALPPAVVDRAKGVTLQALTSALVAHDMPASKQALALMQEEEAGG
                     GGNATVLCSGARLTKAGAAFVNAEMILAGGKWDTFRMLTHPGTAILPAALVAAEITGC
                     SGKAFLAGVAAGYEVMERMAAEFIPTVMSRGFHAGPVFGIFGAAVAAAKIQGLDENQV
                     NGAIAQCVNLAAGNLEGARSGGRSLREGGAVRNALLAVAMAKHGTPGGETTLEGEAGF
                     YHAYAGNNLGRLSYSFTGHTQTELGRITKDLGRNWVFLETLYRIYSTAGFNIAHIDVT
                     ARLCEEHSLTYADIDRIEAVVNWLETEYPSPAFPTPGSDGRPRVGSTQYFTAYGAVER
                     GYPLLRGGQPGPGESDPPEVLDLMQRVTLIPMVRRTLFGPRITIFTRDGRSFTKEGTG
                     REFIWDFEEQARRIQPVVPGLAIGGAQFSDLIDTCRNLETADAAAHRLISLTLP"
     gene            complement(1449..>2036)
                     /locus_tag="VGC82_01390"
     CDS             complement(1449..>2036)
                     /locus_tag="VGC82_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0421959.1"
                     /translation="AMVFGFVKQSGGHIKIYSEPNEGTSVKLYLPRAVGVAVSAGQRS
                     GVPADLPRGSATILLVEDEAGVREVAVAILRSLGYRVLEAQDGEDGLSVFDANAPEID
                     MLLTDVVLPGKVRGRELAERVTATRPEVKVLFMSGYTENSIVHHGRLDDGVHLLGKPF
                     KREQLARKVADVLGTGAGVEAVAEPGKVVNLRARQ"
BASE COUNT          377 a          665 c          636 g          358 t
ORIGIN      
        1 gcactgcccc cagccgtggt tgaccgcgcc aagggcgtca cgctccaggc actgacctcg
       61 gcgttggtcg cgcacgacat gccggcgagc aagcaggccc tggcactgat gcaggaggag
      121 gaagccggcg gcggcggcaa cgcgaccgtg ctttgcagcg gcgcccggct gacgaaggcc
      181 ggagccgcct ttgtcaatgc ggaaatgatc ctggcaggcg gtaaatggga caccttccgc
      241 atgctgaccc atcccggtac ggcaattctg cccgctgccc ttgtggcggc ggagatcacc
      301 ggctgctccg gcaaggcgtt cctggccggc gtcgccgcag ggtatgaggt gatggagcgg
      361 atggccgccg aattcatccc aaccgtgatg tcgcgcgggt tccacgccgg accggtgttc
      421 ggcatctttg gcgcggcagt cgcggcagcg aaaattcagg gcctcgacga gaaccaggtc
      481 aacggcgcca tcgcgcaatg cgtcaatctg gcagccggca atctggaagg cgcacgaagc
      541 ggtgggcgtt cactgcgcga agggggcgct gtacgcaatg cgttgctcgc ggtggcaatg
      601 gccaagcacg gcacacccgg cggcgaaacg acgctggagg gagaggccgg cttctaccat
      661 gcctacgccg gcaacaacct cggcagattg tcatacagct ttaccgggca tacccaaacc
      721 gagctcggtc ggatcaccaa agatttgggc cgcaactggg tttttctgga aacgctgtat
      781 cgcatctact cgaccgcagg cttcaacatc gcccatatcg atgtaacggc aaggctttgt
      841 gaagaacaca gcctgaccta cgcagacatc gaccgcatcg aagcagtggt caactggctc
      901 gagacagaat acccgagccc cgccttcccg acgcccggca gcgatggcag accacgggtc
      961 ggcagcacgc agtattttac tgcctacggc gccgtggagc gcggctaccc gttgttgcgc
     1021 ggcgggcagc ctgggcccgg agagtcggat ccaccggagg tgctggacct gatgcaacgg
     1081 gtgacgctga tccccatggt gcggcggact ttgttcggcc caaggatcac gattttcacc
     1141 agggacggaa gaagcttcac taaagaaggc accggccgcg aattcatttg ggacttcgaa
     1201 gagcaggcca ggcgtatcca gcccgtggtc cctggacttg ccattggcgg cgcgcagttc
     1261 tcagatctga tcgatacctg ccggaacctt gagaccgccg atgccgctgc tcataggttg
     1321 atttcgctta cattgccgtg agcgtttgcc gtccgtcgga tcgtgaccaa tcggtgatgg
     1381 cgcgtcccac cgcagctgga gggctgcccg tcattctcat tggccgccat tctcactgcc
     1441 cgtcattctc actggcgcgc ccgcagattg acgaccttcc caggttcggc gactgcttcg
     1501 acaccggcgc ccgtgcccag aacgtcggcg accttcctgg ccagttgctc gcgtttgaag
     1561 ggctttccca gcaggtggac cccgtcatcc agccggccgt gatggacaat cgagttctcg
     1621 gtgtatccag acatgaacag gaccttgacc tcgggccgcg tggcggtgac gcgctccgcc
     1681 aactcccgtc cgcgcacctt gcccgggagc acgacgtcgg tcagcagcat gtcgatttca
     1741 ggcgcgttgg cgtcgaagac cgacaggccg tcctcgccgt cctgcgcttc cagtacgcgg
     1801 tagccgaggc tgcgcaggat tgccaccgcg acttcccgca cccctgcctc atcttcgacc
     1861 aacaggatgg tcgccgaccc acgcggcaga tcggccggaa cgccgctacg ctgtcccgcg
     1921 gaaacggcaa ccccgacagc gcgcggcagg tacagcttca ccgaagtgcc ttcgttcggc
     1981 tccgagtaga ttttgatatg gccgcccgac tgcttcacga agccaaacac catggc
//
LOCUS       DASWQN010000086         3313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_141602, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000086 DASWQN010000000
VERSION     DASWQN010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141602"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /gene="malQ"
                     /locus_tag="VGC82_01395"
     CDS             complement(<1..1522)
                     /gene="malQ"
                     /locus_tag="VGC82_01395"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY0421960.1"
                     /translation="MSDEVVRDLARRAGIAVEWQDYIGQSHVVEPPVLRRLLAALGLP
                     ADSSRELSSSRRLLTKRSSLVDLPPLVTAVAGRPTRLDVGGSEAQPAELILENGETRH
                     VALLPARGRLRIPAIAETGYHRLRVEDREIVLAVSPARCRSIEDVVPDARLWGVAAQL
                     YGLSHPGDVGIGDLAGAADLARAAGAKGADAIALSPMHALFAANPSQFGPYSPSSRLF
                     LNPLHAAPQLVFGQVESVETGPTNGLIDWPSASANKYALLRRLFESFLDGGDWNGPLG
                     ADFARFRADQGSLLYEHACFEALQAARTPQLRWREWPTDLRDPRGPAVSNFASSQPDE
                     ILYHQFLQWVSDRSVAAAQDTARQSGMRIGLIGDLAVGMDPNGSHAWSRQSDILLGLT
                     IGAPPDLLNPRGQDWGLTGFSPRAMEENGFAPFLATLRAAMRHAGGIRVDHAMGLARL
                     WLIPEGASPADGAYLNFPVGDLLRLLALESVRHNVVVIGEDLGTVPEGFHDMLEQSG"
     gene            complement(1519..>3313)
                     /gene="treZ"
                     /locus_tag="VGC82_01400"
     CDS             complement(1519..>3313)
                     /gene="treZ"
                     /locus_tag="VGC82_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEY0421961.1"
                     /translation="EEDSMTNISASASEFGAKRLPDGAVRFRLWAPAQETVWVAIDGG
                     NLLPMERTSDGWFEAVADVPDGTRYQYRLSDGLMVPDPASTGQADDVHGPSIVQTDTF
                     SWKHPDWLGKPWTEVVLYEVHAGAAGGFRGIRDDLPRLKELGITAIELMPVNDFPGKR
                     NWGYDGVLPYAPDSAYGTRDDLKELVDTAHGLGLMMFLDVVYNHFGPDGNFLNSYAPQ
                     FFRDDVKTPWGPAIDFRRREVRSFFTSNVLMWLTEFRFDGLRFDAVHAIQEQDWIEEM
                     AATVRATIARGRPTVAKDRPMVAQDRPMVAKDRYVHLVLEHHNAASHLRKAVDAQWND
                     DGHNVLHVLLTDESGGYYADYAADPATKLARVLAEGWVYQGEHSAYLEGPRGEPSADL
                     PPYAHVLFLQNHDQIGNRAFGERLTSLTSAAALEAAIALQLLCPQIPLIFMGEETASQ
                     TPFLFFTDHHDELADAVREGRRQEFAGFAEFADPQKRERIPDPNAAETFAASVPRSGS
                     SERFVFYQRLLALRMTEIVPRLAGTKSLGATAIGPKAVFARWRLGDGSTLAIVTNLGS
                     DTVKFQAPAGRLLFATGDAGPMTAAYLEVAE"
BASE COUNT          652 a         1102 c          994 g          565 t
ORIGIN      
        1 tcccgctttg ttcgagcata tcgtggaaac cttcgggaac ggttcccagg tcttcgccga
       61 tcaccaccac gttgtgccgc accgactcca gcgccagcaa ccgcagcaga tctccgaccg
      121 ggaagttcag ataggcgcca tccgccggtg aggcgccttc gggtatcagc cacagccgcg
      181 ccaggcccat cgcatgatca acccttatcc cgccggcatg acgcatggct gcccgcagcg
      241 tcgccaggaa cggcgcaaac ccattttcct ccatcgcccg cggcgagaac ccggtcaggc
      301 cccagtcctg cccgcgcggg ttgagcagat ccggcggcgc accgatcgtc aggccgagca
      361 aaatatcgct ttggcggctc caggcgtggc ttccattcgg gtccatgcca acggcaagat
      421 cgccgatcaa gccgatccgc atgcccgatt gccgcgccgt gtcctgcgct gcggcgacag
      481 agcggtcgga aacccactgc aggaactggt ggtacagaat ttcgtccggt tgcgacgaag
      541 cgaagtttga aaccgcgggg ccgcgaggat cgcgcagatc ggtcggccac tcccgccacc
      601 tcagttgcgg cgttcgcgcc gcctgcaaag cctcgaaaca cgcatgttca tacagcagcg
      661 agccctgatc ggcgcggaaa cgagcgaaat ccgccccaag cggtccgttc cagtcgccgc
      721 catccagaaa ggactcaaac agcctgcgta gcaaagcgta cttgttggcg gatgccgagg
      781 gccagtcgat caggccgttg gtcggtccgg tttcgacact ttcgacctgc ccgaatacaa
      841 gctggggtgc ggcatgaagc ggattcagga acagccggct cgatggggaa taaggcccaa
      901 actgggacgg gttggctgcg aacagcgcgt gcatcgggct gagcgcgata gcatccgccc
      961 ctttcgcccc agccgctcgg gccagatccg cggcgccagc aagatcgccg attccaacgt
     1021 cgcccggatg gctcaggcca tagagctgcg ccgctacccc ccaaagccgc gcatccggaa
     1081 caacgtcctc gatcgagcgg caccgcgccg gtgacaccgc cagcacgatc tcgcggtcct
     1141 cgacccgcaa gcggtggtat ccggtctcgg cgatggccgg gatgcgaagg cgaccccgcg
     1201 ccggaagcag tgcaacgtgc cgagtctccc cattttcgag gatcagctcc gccggctgtg
     1261 cctcgctgcc gccaacatcc aggcgggtcg ggcgtccggc aaccgccgtc accagcggcg
     1321 gcaaatccac caggctgctg cgtttcgtca gcagccgtcg gctggaagac agttcgcggc
     1381 tggagtcggc gggaagcccg agagcggcca ggagacgacg cagaacgggc ggctccacga
     1441 cgtgggattg acctatgtaa tcctgccact ccacagcgat accggcccgc cgggccaggt
     1501 ctcgaacgac ttcatcgctc attccgcaac ctccagatac gcggctgtca tcggaccggc
     1561 gtcgcctgtc gcgaacagca ggcggccagc cggtgcttga aatttcaccg tgtcgctacc
     1621 caagttcgtt acgatcgcca aggtggaccc atcgcccagc cgccatcgag caaacactgc
     1681 cttcggaccg atcgcagtcg cccccaggga ctttgttccc gcaagccgcg gcacgatttc
     1741 agtcatgcgc aacgccagca acctttgata gaaaacgaat cgctcgcttg aaccggatcg
     1801 cgggaccgaa gcagcaaagg tctcggcggc attcggatcg gggatacgct cacgcttttg
     1861 cggatcggcg aattcggcaa atccggcgaa ttcctgccgg cggccttcgc gcacggcgtc
     1921 tgccagttca tcgtgatggt cggtgaaaaa caggaatggc gtctgcgacg ccgtctcctc
     1981 acccatgaag atcagcggta tctgcggaca aagtagctgc agcgcgattg ccgcttccaa
     2041 cgccgccgcc gaggtcagcg aagtcagccg ttcgccgaat gcgcggttgc cgatctggtc
     2101 gtggttctgc aggaacagca catgggcgta tggcggcaga tcggcggacg gctccccgcg
     2161 cgggccttcc agatatgccg agtgctcgcc ctgatagacc catccctcgg cgaggacccg
     2221 cgccagcttt gtcgcgggat cggcggcgta atccgcataa tagccgccgc tctcgtcggt
     2281 caggagcaca tgcaggacgt tatggccgtc atcgttccac tgtgcatcca cggctttgcg
     2341 aagatgggag gcggcgttgt ggtgctccag cacgagatgt acatagcgat ccttggcgac
     2401 catggggcga tcttgcgcga ccatggggcg atccttggcg accgtggggc gaccccgggc
     2461 gatcgtggcg cggacggtgg ccgccatttc ctcaatccag tcctgctcct ggattgcgtg
     2521 cacagcatcg aagcgcagac cgtcgaacct gaattcggtt agccacataa gaacattgga
     2581 tgtaaaaaag ctccggacct cccgtcggcg gaagtcgatc gccggccccc agggtgtctt
     2641 cacgtcatcg cgaaagaact gcggcgcgta ggaattcagg aagttcccgt cgggaccgaa
     2701 gtgattgtaa acgacatcaa ggaacatcat aagcccaagc ccgtgggcgg tatcgaccaa
     2761 ctccttgaga tcgtcgcggg tgccatacgc cgaatccggc gcataaggca gcaccccgtc
     2821 gtaaccccag tttcgcttcc ccgggaagtc attcaccggc atgagttcga tcgccgtgat
     2881 ccccagttcc ttcaaccggg gtagatcgtc acgaatacct cggaagccac ccgcagcacc
     2941 cgcgtgtacc tcatacaaga ccacttcagt ccacggcttg ccaagccaat cgggatgttt
     3001 ccaagaaaaa gtgtcggtct ggacgatact tgggccgtga acgtcgtccg cctgcccggt
     3061 ggatgcaggg tccggcacca tcaacccatc cgataagcgg tactggtagc gcgtgccatc
     3121 cggcacatcc gccacggctt cgaaccaacc gtccgaagtg cgctccatcg gcagcagatt
     3181 gccgccgtcg atggccaccc aaacggtctc ctgcgctggg gcccacagcc gaaaacgcac
     3241 cgccccgtcc ggcaaacgct tcgcgccgaa ttcgctggcg gaggctgaaa tatttgtcat
     3301 ggaatcctct tcg
//
LOCUS       DASWQN010000087         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_9673, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000087 DASWQN010000000
VERSION     DASWQN010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9673"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..817
                     /locus_tag="VGC82_01405"
     CDS             26..817
                     /locus_tag="VGC82_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0421962.1"
                     /translation="MVEDDAGERLGITELLKGDGIDIVTAATGEEAIAQMISSPPDCV
                     VLDLRLPDMSGFDILERIKADSTLCDIPVIVFTGRELSPEEDAQLHTMARSVVVKGVE
                     SPERLLDETALFLHRVFADMALDKQRMLERLHSSDENLVGQKVLLVDDDARNIFALSS
                     VLERRGMQVLTATTGREAITLLEQTPGVAIVLMDIMMPEMDGYETMQVIRGKPEFRRL
                     PIIALTAKAMIGDREKCLEAGASDYLAKPVNTEQLLSALRSWLHR"
     gene            821..2488
                     /locus_tag="VGC82_01410"
     CDS             821..2488
                     /locus_tag="VGC82_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0421963.1"
                     /translation="MMDKVNILLVDDQPAKLLSYEVILEELGENLIKAGSAREALEQL
                     LKNEIALVLTDVSMPETDGFELAAMIREHPRFQSTAIIFVSAIHLTDPDRLRGYAAGA
                     VDYLPIPLVPELMRAKVKVFAELYRKTRELELLNDELERRVAERTAELATANAQLEQR
                     VEERTREREAALAQVHEMQKMESLGQLTGGVAHDFNNLLMAVLGNLELLKKRLPDNPG
                     LVRLVDGAIAGADRGATLTKRMLAFARRQELKPATVQLHSLVEGMEDMLRRTLGSTIS
                     IEIKMDAGLPPVLVDPNQFELALLNLALNGRDAMPNGGRLTIAAHRDEGAALVAGLSS
                     GEYVCILVADSGLGMDEQTLKRATEPFFTTKGVGKGTGLGLPMVHGVVAQSGGAMQIA
                     SQVGLGTSIRLWLPADRTGVAAETRQPVAQSQSMRARRILVVDDDILILSSTAAMLED
                     LGHAVIEACSGSKALEILKADPKVDVVVTDFAMPGMTGLELADLIRQQWPWIPVILAS
                     GYADLMAIQKTPLERLAKPYNQAEIACCIEKVFEERKIVLINAARSA"
BASE COUNT          518 a          727 c          838 g          509 t
ORIGIN      
        1 ccggccccgc cgcaagcagt tgctggtggt ggaagacgat gcgggggaac ggctgggcat
       61 cacggagctt ctaaaaggcg acggcatcga catcgtcacg gcggccacgg gcgaagaggc
      121 gattgcgcag atgatctcgt cgccgcccga ctgtgtcgtg ctcgatctgc ggctgcccga
      181 tatgtccggc ttcgatattc tcgagcggat aaaggccgac agcacgctgt gcgacatccc
      241 ggtcatcgtg ttcaccgggc gcgagctatc gccggaggag gatgcgcagc ttcacaccat
      301 ggcccgcagt gtcgtcgtga aaggtgttga gtcacccgaa cggctgctgg atgagacggc
      361 cctgttcctg catcgggttt ttgccgatat ggcgttggat aagcagcgca tgctggagcg
      421 gctgcatagt tccgacgaaa atttggtagg ccagaaagtt ctgctggtcg atgacgatgc
      481 gcgcaacatc ttcgcccttt caagcgtcct tgagcggcgg ggcatgcagg tgctgactgc
      541 gacgaccggc cgtgaagcaa tcaccttgct ggagcaaaca cctggcgtcg ccattgtcct
      601 gatggacatc atgatgcctg aaatggacgg ctacgagacg atgcaagtca tccgcggcaa
      661 gccggagttc cgtcgtctgc cgatcatcgc gttgaccgcc aaggcgatga tcggtgatcg
      721 ggaaaagtgt ctggaagcgg gtgcatcgga ttatttggcc aagcccgtca ataccgaaca
      781 gcttttatcc gcgcttcgta gctggctgca ccggtagtcg atgatggaca aggtcaacat
      841 cctgttggtc gacgaccaac ctgccaagct gctcagctac gaagtgattc tggaagagct
      901 gggcgagaat ctgatcaagg ccggctccgc gcgagaggcg ctggagcagt tgctgaaaaa
      961 cgagatcgcg ttggtcctga ccgatgtctc catgccggag accgatgggt tcgagctggc
     1021 cgccatgatc cgtgaacatc cgcgcttcca aagcaccgcc atcatctttg tgtccgccat
     1081 tcatctgacc gatccggatc ggctacgtgg ctacgcggca ggcgcggttg attatcttcc
     1141 cattccgctg gtgccagagc taatgcgggc gaaagtgaag gtcttcgccg aattatacag
     1201 aaaaacccgc gaactggagc tgcttaacga cgaactcgag cgtcgcgtgg ccgaacggac
     1261 cgccgaactg gcgacggcga acgcacagct tgagcagcgg gtcgaagagc gtacccggga
     1321 gcgtgaggcc gcgctcgccc aggtccacga gatgcagaag atggaaagcc tgggccaact
     1381 caccggcggg gtggcgcatg atttcaacaa tctccttatg gcggtcctgg ggaatctgga
     1441 gctgctcaag aagcggctgc ccgacaaccc tggcttggta cggttggtcg acggcgcgat
     1501 cgcaggtgcc gatcgcggcg ccaccttgac gaagcggatg ctcgccttcg cgcggaggca
     1561 ggagttgaaa cctgcgaccg tacagctcca ttctctggtt gagggcatgg aagacatgct
     1621 gcgacggacc ttggggtcca cgatcagcat cgagatcaag atggatgccg gtcttccgcc
     1681 ggtcctggtc gaccccaacc agttcgagtt ggcgctgctc aatctggcgc tgaatgggcg
     1741 cgatgcgatg ccgaacggtg gacgactgac cattgcggcg catcgggacg aaggtgcggc
     1801 tctcgttgcc gggctttcga gcggcgagta tgtctgcatc cttgtcgccg attccggatt
     1861 gggaatggac gaacagacgc tcaagcgcgc caccgagccg ttcttcacaa ccaaaggcgt
     1921 ggggaagggc acgggactcg gtctgcctat ggtgcatggg gtggtagcgc aatcgggcgg
     1981 cgcgatgcag attgcaagtc aggtgggtct gggcacctcg atccggctat ggctgcctgc
     2041 ggatcgcacc ggcgtggcgg ccgaaacccg ccagccggtg gcgcagtccc agagcatgcg
     2101 cgctcggcgg attctggtgg tcgatgatga cattctgatc ttgtccagca cggcggccat
     2161 gttggaagat ctgggccacg ccgtcattga ggcatgttcg gggagcaagg ctctggaaat
     2221 tctcaaagca gatcctaagg tcgatgtcgt cgttactgac ttcgcgatgc ctggtatgac
     2281 cggactggaa ctcgccgact taatacgcca gcaatggccc tggattccag tcatcctggc
     2341 cagcggctat gctgatttga tggctattca aaagaccccg cttgagcggc tggcgaagcc
     2401 atacaaccag gcggaaatcg cctgctgcat cgaaaaagtc ttcgaggagc gaaagatcgt
     2461 tctgatcaat gcagcacggt ccgcgtagcc attcttgcta tctcggttga taccctcgca
     2521 acggctcgat cgagcgcgta ggcgagcgcc gcgatcccag gtttcgacga tttccggcgg
     2581 gtcggatggc tg
//
LOCUS       DASWQN010000088         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2516865, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000088 DASWQN010000000
VERSION     DASWQN010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2516865"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VGC82_01415"
     CDS             <1..354
                     /locus_tag="VGC82_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0421964.1"
                     /translation="RRPSKEPLDKGGWSMFPAGAPGPEYVDPLLANTLRSNGAKAWFG
                     WPDDPALEAAYDSWVDADNDAERRKQEVAFQTAAFRSVPSIPLGQYLPHAAWRSNVTG
                     LVKGSAPVFWGARKA"
     gene            complement(586..1341)
                     /locus_tag="VGC82_01420"
     CDS             complement(586..1341)
                     /locus_tag="VGC82_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421965.1"
                     /translation="MQQQHLPFINARYWTAIVAASMCGANTGDFASRILHLGHTRGLL
                     PLALIFGLVLWAEARNKSATELYYWMAIIALRTAATNLADLATHDLKLGYVAVELGLA
                     GLMILILLVDRARGSTRRGVRDDDGQWQNLPATDASYWIVMLAAGTLGTALGDWVADE
                     LGLGLGLGSVVLVAVLGVVILLSTRYGRMTKPWYWLSIVAARTAGTTLGDFVASRHGL
                     DFGLPISTLSTSLLLVAILVLWKNDRASRLREV"
     gene            complement(1563..>2224)
                     /locus_tag="VGC82_01425"
     CDS             complement(1563..>2224)
                     /locus_tag="VGC82_01425"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEY0421966.1"
                     /translation="ALGDARNWDVFESLLRGGPCSAFPSETGFASLTADAATQREAAY
                     AAVADLLARADTTRFVLSVEAFVARRGWRNALPGEALSSLTEPVTGFAAESLARLHRR
                     VRKRGKALLELPPEERHGVRIALKNLRYAADFFGDLFGPADAVKSYVREAARLQDALG
                     SFNDMVMVTALVRQLDIDAALPARAAGIISGWYAHGARANDADLHDVWRGFRKVRPFW
                     T"
BASE COUNT          451 a          698 c          706 g          369 t
ORIGIN      
        1 cgccgcccat cgaaggaacc gttggacaag ggcggctggt cgatgttccc agccggcgcg
       61 ccaggtcccg aatatgtcga tccgctgctg gcgaacacgc tgcgcagcaa cggcgcgaaa
      121 gcctggttcg ggtggccgga cgacccagcg ctggaggcgg cctacgattc ctgggtcgat
      181 gccgacaacg acgccgaacg ccgcaaacag gaggtcgcgt tccagacagc ggcgttccgg
      241 tcggtgccaa gcattccgct tgggcaatat ttgccgcatg cggcgtggcg gtcgaatgta
      301 acgggattgg tgaaaggatc ggcgccggtc ttctggggcg cgcgcaaggc gtagccaacg
      361 ataggctatc gagccgacac catgcgcctc gatcctaacg ttgcacgttg agtgacggtc
      421 gaattgcttt ggcgatgatg aaatcggtcc aatccgaagt agctcggtcc atggttcgcg
      481 aaggtatctc gtgagaaaac gatttacgat cgaaatgccc cattcggcgg cagacatcgc
      541 tgctatgatg ccggtgcggt cgccgcgctc gcgttcgatt gcactctaca cctcgcgcaa
      601 tcggctggct ctgtcgttct tccacagcac caggatggca accagcaaca ggctcgtgga
      661 tagcgtgctg atcggcaggc cgaaatcaag cccatgacgg ctggctacga aatccccgag
      721 cgtcgtgccg gcagttcgcg cggcgacaat gctgagccag taccaaggct tcgtcatgcg
      781 tccgtagcgc gtcgaaagca gtataacgac accaagaacc gcaaccagga cgactgatcc
      841 taggcccaga ccaagcccaa gctcgtcggc aacccaatcg ccaagtgccg tccccaacgt
      901 accggctgcc agcatgacga tccagtaact ggcgtccgtg gccggcagat tttgccattg
      961 tccatcgtca tcccgcacgc ctcggcgcgt cgagccgcgc gcacgatcga cgaggagaat
     1021 caagatcatt agtcccgcca agcccagttc gacggctaca taacccagct tcagatcatg
     1081 ggtggcgagg tcggcaaggt tcgtcgccgc ggtgcgcagg gcgatgatcg ccatccaata
     1141 gtagagctcc gtcgcgcttt tgttccgcgc ttcggcccat aaaacgagtc cgaaaatcag
     1201 cgcgagaggc aggagcccgc gcgtatggcc cagatgcagg atacgggaag caaagtcgcc
     1261 ggtgttggcg ccgcacatgc tggcagccac gatggcggtc cagtaacggg cattgataaa
     1321 cggaaggtgt tgctgctgca tggttgtccg gaattacggg tggggttgaa ggtcgcgcat
     1381 cgatggcgaa gccgcttgct tcaggcaagg cggcagatag caaaccgctc gcaccgacac
     1441 cgtggcgtaa acatggccgg cccgaggcaa gcgagagcgg gctgtgggcg cttgattgcc
     1501 actgatggac cgtgccgggc ctatgcagga aaggccgcct cgacaggaca ggcagagatc
     1561 catcaggtcc agaatggccg gaccttgcgg aagccgcgcc aaacgtcgtg cagatcggcg
     1621 tcgttcgccc gggcgccgtg ggcataccag ccgctgatga tccccgcggc ccgtgccgga
     1681 agggcagcat cgatatccag ttgccgcacc agcgcggtca ccatcaccat gtcgttgaag
     1741 ctgcccaagg catcctgcag cctggcagct tctcggacat aagacttgac ggcgtcagcg
     1801 ggaccgaaca ggtcgccgaa gaaatcggcc gcatagcgca ggttcttgag cgcgattcgg
     1861 acgccgtggc gctcctccgg cggcaactcg agcagcgcct ttccacgctt gcgcacccgc
     1921 cggtgcaaac gtgccagact ctctgccgcg aagccggtca cgggttccgt caggctggac
     1981 aaggcttcgc ccggcagggc gttgcgccag ccacgccggg cgacgaaggc ttccacggac
     2041 aatacgaaac gggtggtatc cgcacgtgcc agcaggtcag cgacggccgc atacgccgcc
     2101 tcccgctgtg ttgccgcatc ggcagtcagg ctcgcgaacc cagtttcgga cgggaacgcg
     2161 ctgcagggcc cgccgcgcag aagactctcg aacacatccc agttgcgagc atctcccagt
     2221 gccg
//
LOCUS       DASWQN010000089         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1988935, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000089 DASWQN010000000
VERSION     DASWQN010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988935"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1615)
                     /locus_tag="VGC82_01430"
     CDS             complement(<1..1615)
                     /locus_tag="VGC82_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0421967.1"
                     /translation="MFRLVAYPTRSHRLGLVVPASLVCALAMAAFDPPFSTGIAAHVA
                     ATSFSKLAQGGAAFGFPGITAAVIVGSAAVLILMLAGLIFGRHRRQALAIQARRFRTL
                     ADATFEGLVFERDGRIVDVNRMMCRLAGSDAATLIGLRLADLIPGMAITPRRNEPAME
                     FALLLPDGQTRPVEAIWRDDFDHLGRRGHIVAIRDLSQQKAAEARIEQLANFDSLTGL
                     ANRDLFEQQLQNALDLRGQSPNGVALLYVGLDRFETLNEALGPLPGQQILLQTARRLS
                     AIVQLADIVARLGHDEFAVIRPMTEPAGSVVLAERIVTQMALPFLVDGQPVGLTASVG
                     VARYPADGTTAGQLMKYAALALLQAKHDGSGQWRHFEPEMDMALRTKRSLEHDLRAAL
                     REGQFSLHYQPFIAMQTGACAGYEALLRWNHPERGQISPVDFIPWAEKSGLIVPIGSW
                     VLATACAEAVSWDNPAIISVNLSPIQFVQPGIVAIVADVLRRTGLPAERLELEITEGT
                     LMDDTENALRTLTALKALGVKIAMDDFGTG"
     gene            complement(1720..2196)
                     /gene="pal"
                     /locus_tag="VGC82_01435"
     CDS             complement(1720..2196)
                     /gene="pal"
                     /locus_tag="VGC82_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY0421968.1"
                     /translation="MLAGCSSSDDSKNAGGSGGVTGVSGSGSGSGGPGGAGRAALGSE
                     EDLVQSAGDRIFFDTDRNTLTPQATATLDRQAGWLQQYPQVNVWVAGNCDERGTEEYN
                     LALGQRRAAADRDYLVAHGINRSRIETISYGKSRPIDAASTPQAWAQNRNAITSVR"
BASE COUNT          459 a          820 c          820 g          471 t
ORIGIN      
        1 agccggttcc gaagtcatcc atggcgatct tcacccccag cgccttcagc gccgtcaggg
       61 tgcgcagcgc gttctcagtg tcgtccatca acgtgccctc ggtgatttcc agctccagac
      121 gttccgctgg caaaccagtc cgccgcagta catcggctac gatggcgacg atgccgggct
      181 gcacgaactg gatcggggac agattgaccg atatgatggc cggattgtcc catgacaccg
      241 cttcggcgca ggcggtcgcc aatacccagc ttccgatcgg gacgatcagc ccggatttct
      301 ccgcccacgg tatgaaatcg accggggaaa tctggccgcg ttcgggatga ttccatcgca
      361 gcaatgcctc gtagccggca catgcgccgg tctgcatcgc tatgaagggc tgatagtgca
      421 ggctgaattg accctccctg agagcggccc gcagatcgtg ttccagcgat cgcttcgtcc
      481 gcagcgccat gtccatctct ggctcgaaat gccgccattg gccgctgccg tcatgctttg
      541 cctgaagcag ggccaatgcg gcatatttca tgagttgtcc cgctgttgtt ccgtcagccg
      601 gatagcgcgc gaccccgacg ctggcggtca gtcctaccgg ctgcccatcg accaggaacg
      661 gcagcgccat ctgggtgacg atccgctctg cgagcacgac cgaacccgcc ggctccgtca
      721 ttggccggat aaccgcgaac tcatcgtgcc ccaaccgagc gacgatatcg gctaactgta
      781 cgatggcgct gagccggcgc gcggtctgga gcaggatctg ctggccgggc aaagggccca
      841 gcgcttcgtt cagcgtttcg aagcggtcca aaccgacata gagcagcgcg acgccattcg
      901 gactttgtcc tcgtagatca agcgcgtttt gcagttgctg ctcgaaaaga tctcggttcg
      961 caaggcccgt tagcgagtcg aagttggcca gttgctcgat tcgtgcctcg gcagccttct
     1021 gctgggacag gtcgcggatc gccacgatat ggcctcgccg gccgagatgg tcgaaatcgt
     1081 cccgccagat cgcttcgacc ggacgggtct ggccgtcggg gagaagcaag gcgaactcca
     1141 tcgcgggttc gttccgccgc ggtgtgattg ccattcctgg gatcagatcg gctagccgca
     1201 ggccgatcag cgtcgcggca tcgcttccgg ccaagcggca catcattcgg ttgacgtcca
     1261 cgatacggcc gtcgcgttcg aacaccaatc cctcgaaggt cgcgtcggcc agggttcgga
     1321 agcggcgcgc ctgaatggcg agagcctgcc ttctgtgccg gccgaaaata agccctgcca
     1381 gcatcaggat cagcacggcc gcgctgccga cgatgacggc cgcggtgatt ccgggaaagc
     1441 cgaacgcggc gccgccctgc gcgagcttcg agaagcttgt cgcggcaaca tgggccgcga
     1501 tgccggtaga aaacggcggg tcgaaggccg ccatagccag cgcgcagacc agggacgctg
     1561 ggacgaccag ccccagccta tggctccgcg tgggatatgc gacaagccgg aacatgatga
     1621 tcccttccgt tcaggaaagg atcatcggct tcaagtatta tcagacggtt aacgatagcg
     1681 gtgttttacc cccgcttttc tccgcggccc gcgtgaagat cagcggaccg aggtgatcgc
     1741 gttgcggttc tgcgcccagg cctgcggcgt tgacgcggca tcgatgggcc gggatttgcc
     1801 gtagctaatg gtttcgatgc ggctgcggtt gatgccgtgg gccaccaggt aatcgcgatc
     1861 ggccgcggcg cgccgctggc cgagggccag gttgtactcc tccgtgccgc gctcatcgca
     1921 gttgccggcg acccagacat tgacctgggg atattgctgt agccagcccg cctgccggtc
     1981 gagggtggca gtcgcctgcg gggtcagcgt attgcggtcg gtgtcgaaga agatgcggtc
     2041 gccggccgac tgcaccaggt cttcctcgct tcccagcgcg gcccgtccgg cgccgcccgg
     2101 gccgccggat ccggatccgg agccggatac gccggtaacg ccgcccgatc cgccggcgtt
     2161 tttggagtca tcggatgacg agcagccggc cagcaaagcc gcgcagagca ggcacgcaag
     2221 agtccaatgt ttcacgaagc atctccagtc atgtgtttgg ccgggtcatc atagcggcat
     2281 cgccacgatt cgcggacgtc agcgggtcac gccagtgttt gtaaagggag tcgtgggtgg
     2341 cggtgccaag cacggccgtg acacatgggc gccagacggc aaaaccggct cacacctttt
     2401 cagccagcct gacgcgcttt agtccgccag agcggcataa accatgtcgc ggaagaggat
     2461 ccggtaatgt tcgcgttgca gcggcgcctg gaccatcagg accgcatata gccgttccgc
     2521 cggatcgacc cagaacgtgg tcccggccgc tccgccccag tagtagttgc
//
LOCUS       DASWQN010000090         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1329459, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000090 DASWQN010000000
VERSION     DASWQN010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329459"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /gene="priA"
                     /locus_tag="VGC82_01440"
     CDS             <1..1059
                     /gene="priA"
                     /locus_tag="VGC82_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY0421969.1"
                     /translation="LSRHGGASMPRIDLVDLRETPPDRGRFLSPPLIEAAKQTLARGE
                     QAMLFLNRRGYAPLTLCRHCGHRMQCPNCTAWLVEHRTRSRLLCHHCGHTVPIPPECP
                     VCSAKDSLTPVGPGIERITEEAALVFPDARRLIMASDTIAGPYAAAAAAHAIEDREVD
                     LIIGTQLVAKGWHFPHLTFVGVVDADLGLAGGELRASERTVQLLHQVAGRAGRAEAPG
                     RVMLQTFIPDHPVMQALLQGDLDAFMQSEAESRRDGEWPPYGRLAALIVSADTAIMAD
                     TVARDLGWAAPSGDGIIVLGPAPAPMSLLRGRHRRRLLLKVRRDVAVQPILRAWLAAV
                     RVPRGGRIDVDVDPISFL"
     gene            1421..>2486
                     /locus_tag="VGC82_01445"
     CDS             1421..>2486
                     /locus_tag="VGC82_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY0421970.1"
                     /translation="MLRYLGDTKLATRIFIVMGLLALTSVMVGWSGLHTARIYSEKVA
                     AMQAASARAIIGEQVNGLINAVVMDSRGIYGAKDAAQIEKFSQPLLENLRRIDKRMSD
                     WSDLVSGEGRPLFDQCAAAVGNFIGLRVAIVEAGRTQGAAAADKLGNNDMARANRETV
                     NRDVVALAARNAADVERVVVELARFHETMATLVPALTAGGIVLVAVLAVLLVMRGITG
                     PLGRITVATGQVAEGKLDIEVPARGKTDEIGRIASALETFRLQAIENRALTGVRLAEQ
                     QRAEAEKQTALTNMAETIEATANAAMMEIGQRNVATTLAAEKMLELAGSIGGAASGAA
                     EAASVASANAQTIAEAAEQLA"
BASE COUNT          442 a          766 c          874 g          404 t
ORIGIN      
        1 ctgagccggc acggcggcgc ctcgatgccg cggatcgacc tggtggacct gcgggaaacg
       61 ccgccggatc gcggccggtt tctgtcgccg ccgttgatcg aggcggcgaa gcagacgctg
      121 gcgcgcggcg aacaggcgat gctgttcctc aaccgccgcg gctatgcgcc gctgaccctg
      181 tgccggcact gtgggcaccg gatgcaatgc ccgaactgca ccgcctggct ggtggagcac
      241 cggacgcgaa gccggcttct gtgtcaccat tgcgggcaca ccgtgccgat cccgccggaa
      301 tgcccggtat gttcggcgaa ggacagcctg acgccggtcg gccccggcat cgaacggata
      361 actgaagagg ccgcactggt atttcccgac gcgaggcggt tgatcatggc cagcgacacc
      421 atcgccggac cgtacgcggc ggcggcggcg gcacatgcca tcgaggacag ggaagtcgat
      481 ctcatcatcg ggacgcaact ggtggccaag gggtggcact ttccgcactt gacgttcgtc
      541 ggcgttgtcg atgccgattt gggcctggcc ggcggagaac tgcgggcgtc cgaacgcacg
      601 gtgcagttgc tgcaccaggt cgccggccgg gccggccgtg cggaggcgcc aggacgggtg
      661 atgctgcaaa ccttcatccc cgatcatccg gtgatgcagg cgctgctgca gggcgacctg
      721 gacgcattca tgcaaagcga ggccgagagc cggcgggatg gcgaatggcc gccgtatggc
      781 cggttggcgg ccctgatcgt cagtgccgac accgcgataa tggcggacac ggtggcgcgc
      841 gatctcggct gggccgcgcc cagcggcgac gggatcatcg tgctcggacc ggcgccggca
      901 ccgatgtccc tgttgcgcgg gcggcatcgg cggcgcttgc tgctgaaggt gcggcgggat
      961 gtggcggtgc aaccgattct gcgggcatgg ctggccgcgg ttcgcgtgcc tcggggtggg
     1021 cgtatcgatg tggatgtgga cccgatctcg tttctataag cctttcggcc ggatggaagc
     1081 ctcttgaact gcagcccgaa gggctgactg gcatgtgcct agggttggca tggcggctgt
     1141 gtcaaaccga taagataccg accgcccctg acccggcgac ggtcagcatt tagggccgtt
     1201 ggtatgagcg ccctcgcgct gctttgcact ggacgtcaat agcctgatga agaaacgaaa
     1261 acgacgtctt ggcggcagcc acggcaacga tcctgaacca gtccggtgcg caccgcgatt
     1321 gcccgaagca gtgaacgggc ggagttaacc gttccttaat cttgcgccgc tatcagccgc
     1381 cacaggttta cggccaacct tcctttagga tgcatcaggt atgctgcggt atttgggcga
     1441 tacgaagcta gcgaccagga ttttcatcgt catgggcctt ctggccctga cgtcggtgat
     1501 ggtcggttgg tcgggcctcc acaccgcgcg gatctacagc gagaaagtcg ccgccatgca
     1561 ggccgcgtcg gcacgggcga tcatcggcga gcaggtgaat ggactgatca acgcggtcgt
     1621 catggattcg cgcggcatct acggcgcgaa ggatgccgcc cagatcgaga aattctccca
     1681 gccgctgctg gagaacctgc ggcgcatcga caaacgtatg tccgactggt cggaccttgt
     1741 ttcaggtgag gggcggccgt tgttcgatca atgcgcggca gcggtcggca acttcatcgg
     1801 cctgcgcgtc gcgatcgtgg aggccggccg cacccagggc gccgccgccg cggacaagtt
     1861 gggcaacaac gacatggcgc gcgcgaaccg tgaaaccgtc aaccgggacg ttgtggcgct
     1921 ggcggcacgc aatgccgcgg atgtcgagcg cgtggtcgtt gaactcgcgc gattccacga
     1981 aaccatggcg acgctggttc cggccctgac ggcgggcggc atcgtcctgg tcgcggttct
     2041 ggccgtgctg ctggtcatgc gcgggatcac cgggcccttg ggccggatca ccgtggcgac
     2101 cgggcaggtc gcggagggca aactcgacat cgaagtgccc gcacgcggca aaaccgacga
     2161 aatcggccgc atcgcctccg cactcgaaac gttccgactg caggcaattg aaaaccgtgc
     2221 gctgaccggc gtgcggctcg ctgagcagca gcgtgccgaa gcggaaaagc agacggcgct
     2281 gaccaatatg gcggaaacga tcgaggcgac cgccaacgcc gcgatgatgg agatcgggca
     2341 gcggaacgtg gccacgacac tggccgccga gaaaatgctg gaactggccg gaagcatagg
     2401 cggggcagcg agcggcgcgg cagaggccgc atccgtcgct tcggcgaatg ctcaaaccat
     2461 cgccgaggcc gccgaacaac tagcgg
//
LOCUS       DASWQN010000091         6668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1329771, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000091 DASWQN010000000
VERSION     DASWQN010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329771"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            515..1333
                     /locus_tag="VGC82_01450"
     CDS             515..1333
                     /locus_tag="VGC82_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0421971.1"
                     /translation="MPPLDKVYDYSQYGGLRVEKQNKVMTITLNAPESMNAFSAEMHY
                     SMSRIWEDVQDDRDVNVVVLTGAGRAFSAGGNLIAMQQKIDRPELWDATSLPEAKRII
                     FRMLECDKPIIARVNGHAVGLGATVALMCDIIIAADTAKIGDPHVNAGLVAGDGGALI
                     WPQLIGFARAKEYLFTGDLMTAAEAHRIGLINHVVPPDELDEKVYGLARRLAAGAMKS
                     IRRTKQVINIPLRQIAHASMDLSLSLETQSNLSKDHQEAVAAFTAKRTPKFTGE"
     gene            1657..2820
                     /locus_tag="VGC82_01455"
     CDS             1657..2820
                     /locus_tag="VGC82_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HEY0421972.1"
                     /translation="MPRGLCTDCGISRTEDPKRCGKACQFIKPDYPGLETLVHGRPRD
                     PARPDELFFGPFRRMLRASLAAPLANAQWTGIATRIAERLLETKAVEAVLTMAPDPND
                     RWRPVPVLVTEAAGMAHCRGMRMGYAPLLALLEPARARGYTRLAVIGIPCQVYALRAL
                     EQELGFERLYVIGTPCSDNTTTERFHEFLGLLAADPDTISYLEFRADYHVELRFFDGR
                     IKEIPFLQLPISKLPADFFPLTCRTCVDYTNVLADITVGYMGGGGEQWLLVRNERGEE
                     LLAALGNEIRTAAPGSAGKRKAPVTGFMKNAERAAGGLPLRAMPDWLRPVVGWLMPRI
                     GPRGLEFARARIEMKAIETVLHLRRAHPRRLRNMVPDHVWALVKPYGLTRDPE"
     gene            2817..3734
                     /locus_tag="VGC82_01460"
     CDS             2817..3734
                     /locus_tag="VGC82_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0421973.1"
                     /translation="MMPMVVLLVILGILGGGAWLYTPDKPREALEAIYAEPPSAFLPI
                     AGLRMHVRDTGPKTAPAVILLHGLGSSLQTWEPWAQALSARFRVIRCDLPGFGLTGPD
                     PTGDYSDDRAIFVLGALMDALSVGRVSLIGNSMGGKLAWQFAAAHPERVNKLVLISPD
                     GFASPGFEYGKPATVPMIARLLPYTLPTAMLRMNLSPAYADKSRLAETTVTRYRDLML
                     APGVRRALLERTAQVRLEPPEPWLRRIEAPTLLVWGEKDALIPFSNARDYLEAIPHVR
                     SVSFADLGHVPQEEDPAASLPPVEAFLAE"
     gene            4033..4455
                     /locus_tag="VGC82_01465"
     CDS             4033..4455
                     /locus_tag="VGC82_01465"
                     /inference="COORDINATES: protein motif:HMM:NF020360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HEY0421974.1"
                     /translation="MPLVLDAFRNALAALRAVQAKSEDAAFMATLDTVTREAIRSGAI
                     QHFEFTFELAWKFMRRRLEADIGRSSVDGIPRRDLFRIGAENGLIDDVEAWFQYHLAR
                     NRTSHTYDPSIVASLYARSLDFANDAVALLAVLEARNV"
     gene            4448..4756
                     /locus_tag="VGC82_01470"
     CDS             4448..4756
                     /locus_tag="VGC82_01470"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY0421975.1"
                     /translation="MFDISPRDLAIVQTILRKHVPDHEVWAFGSRTKGTARRYSDLDL
                     AIITATPLSYQIRGDMEADLSESDLPFRVDILDWATTQENFRRIVEQDRVVVQPGKQT
                     "
     gene            complement(4803..5585)
                     /locus_tag="VGC82_01475"
     CDS             complement(4803..5585)
                     /locus_tag="VGC82_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0421976.1"
                     /translation="MDVRLDGRTAIITGSSKGLGLAMAKEFAASGANVAILARSPDGL
                     AAARATVSEGASGKAATFQCDVSKAADISKTFEAVINTFGQVDILINNAGMSRAMPSD
                     QISDDIWQEDLDLKLFAAIRLARLCWPGMKARKWGRIINVLNTGAKAPRANGAPTAVS
                     RAAGMALMKVMSAEGAPHGILVNGMLVGQIVSDQIARRYKTEGSNEPFEAYVAKAGAG
                     IPVGRMGKAEEFAAMACFLCSDHAGFTTGTAINMDGGASPVV"
     gene            5674..>6668
                     /locus_tag="VGC82_01480"
     CDS             5674..>6668
                     /locus_tag="VGC82_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEY0421977.1"
                     /translation="MSSFEFVSALLLLAAILGIVNHLYVHLPRTIGVMVGSLILSIII
                     ILIDRSIDVVELREWWQQLVVSTDLPRVFLDGLLAFMLFAGSLHVDMDSLRAHKWTVL
                     VLATVGVVMATALYGFGIWFIFRDTVPLAWCFALGALLAPTDPIAVGGLLKQAGLPPG
                     LLAVVNGESLFNDGVAVVVFAVTLEWANGHVATPATMAADLLIEAGGGIVLGLATGYL
                     AYRALRLIDEPGLELTITLALVTVTYSVASALHVSGPLAVVVAGLLTGHRSTRFAMTD
                     RSRREVILFWDMMDELLNTVLFLLMGFALLSVDIDTHLLLGAAGGIGLSLVTRLI"
BASE COUNT         1213 a         2097 c         2081 g         1277 t
ORIGIN      
        1 attgaataac gcgattgaaa cagtctcaat aaaagcgcaa ttgtatcgta gtcgcgatgc
       61 tcattggcgg cgatgaagcg aaggcggctg agtcataagt ggcgggccca agtttatggc
      121 tggaaacacg ccggtggcgt agccgtagat gatccgtcca agcaggggtt gcgggtggta
      181 gggcgcctcg tccaacccac ggtagcgacc ggtcatggcg cagatcaagg ccttcgatca
      241 cctcaaccac gaaccggcca gggccattgt ggcaaccgtt catccaccga tggcggcatc
      301 acaagcccgt gtcgcggtcg atcaggcgga agttgctcat gcgccaggtg tccagactgg
      361 tatgcgaagc gcgcgcccgt cgaccgcaca cctccgtgtc gcgttaagtc cgacgggctg
      421 ccagcagatg acatcagtga cgagccggcc ggttgacatc ataatccgcg cgaccgccgt
      481 tgaccggcgc aagcaacagc agggagcgaa cgccatgccg ccgctagaca aagtatatga
      541 ctacagccag tacggcgggt tgcgggtcga aaagcagaac aaggtgatga ccatcacctt
      601 gaacgctccg gaatcgatga acgcgttctc cgccgagatg cattattcga tgtcgcgtat
      661 ctgggaggac gtgcaagacg accgggatgt aaacgtggtt gtcctgactg gtgccggacg
      721 cgcgttctcg gcgggcggca atttgatcgc aatgcagcag aagatcgatc ggccggaact
      781 gtgggacgcc accagcctgc cggaagcaaa acgcatcatt ttccgcatgc tggaatgcga
      841 caaaccgata atcgctcggg tcaacggtca tgccgtcgga ctaggcgcca cggtggcgct
      901 gatgtgcgac atcatcatcg ccgccgatac ggccaagatc ggtgacccgc atgtaaacgc
      961 gggtttggtg gctggcgacg gcggagcgtt gatctggccg cagttgatcg gtttcgcgcg
     1021 ggccaaggaa tacctgttca ccggcgacct gatgaccgcg gccgaggcgc accggatcgg
     1081 gctgatcaac catgtcgtgc cgccggacga actggatgag aaagtctatg gccttgcacg
     1141 ccggttggcc gccggagcga tgaagtcgat caggcggacg aagcaggtga tcaacattcc
     1201 gctgcggcaa atcgcacacg cgtcgatgga tctcagcctc agcctggaaa cgcagtcgaa
     1261 cctttcgaaa gatcaccagg aggcggtggc ggccttcacc gccaagcgaa cgccgaagtt
     1321 caccggcgaa tagaaggcgt cgggtgcttg cactcgttgc ccgaaagccg aattgtcgct
     1381 agagcgtgat cacgctgggc ggaacgtccg tcgtccaaag gacacgctgt tcgcaccatg
     1441 catgtgaatc acgcgaagga cacgctgttc gaaccatgca tgtgaaggac gcgctgttcg
     1501 cacgatgcgt atgatccgca cgataaaaca aggatataaa ccattgcggc ctgcacaatc
     1561 aggccgggaa tggtctagcg gggcagcggt gggggcagcg ggcacggcaa tcaacagaaa
     1621 cccgactctg tctgggctcc tccactgcgg gcagcattgc cgcgtgggct gtgcaccgat
     1681 tgcggcattt ctcgcaccga ggatcccaaa cgctgcggca aggcctgcca gttcataaag
     1741 ccggactacc cgggcctgga aaccctggtc catggccgtc ctcgcgaccc cgcccgaccc
     1801 gacgaactgt tcttcggccc gttccgccgc atgttgcgag catccttggc cgcaccgctg
     1861 gcaaatgcgc aatggaccgg gattgccacc cgcatcgccg aacgcctgct ggaaaccaag
     1921 gctgtcgagg cggtgttgac catggcgccg gacccgaacg accgctggcg tccggtaccg
     1981 gtcctggtga ccgaagccgc cggcatggcg cactgccgcg gaatgcggat gggttacgcg
     2041 ccattgctgg cgttgcttga acccgcacgg gcgaggggct acacgcgcct ggcagtgatc
     2101 ggcattccct gccaggtcta tgcactgcgc gctctggaac aggaactcgg gttcgaacgc
     2161 ctatacgtta tcggcacgcc ctgttccgac aacacgacga cagaacggtt ccacgagttc
     2221 ctcgggttgc tcgccgccga tcccgacacc atctcctatc tggagttccg cgccgactac
     2281 catgtggaac ttcgtttctt tgacgggcgc atcaaggaaa tcccgttttt gcaactaccg
     2341 atttcgaagt tgccggccga tttcttcccg ctgacctgcc gcacttgcgt tgactacacc
     2401 aacgtcctcg ccgacatcac ggtcggctac atgggcggcg ggggcgagca atggctgctc
     2461 gtccgaaacg agcgcggcga agaattgctt gccgccttgg gcaatgaaat ccgcaccgcc
     2521 gcacctggca gtgccggcaa acgcaaagcc ccggtcaccg gattcatgaa gaacgccgaa
     2581 cgcgccgccg gcggcctgcc gctgcgcgcg atgcctgact ggctgcgtcc cgtcgtcggc
     2641 tggctgatgc cccgcatcgg tccgcgcggc ctggagttcg cccgggcgcg gatcgagatg
     2701 aaggcgatcg agacggtgct ccacctgcgc cgcgcccacc ccagacgcct gcgcaacatg
     2761 gttcccgacc acgtttgggc gctggtgaag ccgtatggat taacccgaga tcccgaatga
     2821 tgccgatggt cgtgttgctc gtgatcctcg gaatcctcgg cggcggtgcc tggctctata
     2881 caccggacaa gccacgggaa gcactggagg cgatttacgc cgaaccgccc tcggcgttcc
     2941 tcccgatcgc cggtcttcgg atgcatgtcc gcgacaccgg gccgaagacc gcgccggcgg
     3001 tgatcttgct gcacggcctt ggatccagtc tgcagacctg ggagccttgg gcgcaggcgc
     3061 tgtcagcccg cttccgggtg atacgctgcg acctgcccgg cttcggcctg actggacccg
     3121 acccgaccgg cgactacagc gacgaccgcg ccatcttcgt gctgggggcg ttgatggacg
     3181 cgctatcggt cgggcgcgtg tccttgattg gcaacagcat gggcggcaaa cttgcctggc
     3241 agttcgcggc ggcgcatccc gagcgggtga acaaactggt gctgatctcc cccgatggct
     3301 tcgccagtcc cggcttcgaa tatggcaagc cggctaccgt tcccatgatc gcccggttgc
     3361 tcccgtacac gctgccgacc gcgatgctgc gtatgaacct ttcaccggcc tatgccgaca
     3421 aatcgcggct ggcggagacg accgtcacgc gctaccgcga cctgatgctg gcccccggcg
     3481 tccggcgggc cctactggaa cgcacggcgc aggtgcgact ggaaccgccg gagccgtggc
     3541 tgcggcgaat cgaggcgcca accctgctcg tctgggggga aaaagacgcg ctgatcccgt
     3601 tcagcaacgc tcgcgactat ctggaggcca ttccgcatgt tcggtcggtc tcgttcgcag
     3661 acctcggcca tgttccgcag gaggaagacc cggccgcatc gctgccaccg gtagaggcgt
     3721 tcctcgcaga ataacgcctt tttgtcgcgg caagcgggga aggctgttac agatcgtcct
     3781 gccgatggat gtcttgccgg gcgaatgcca gatgtccccg gagtgccgtt tcgcctgctc
     3841 aagtacggga cagcggcatc agcggacggt gaagctggcc cgccacagga ggggcgcgtt
     3901 gcagcaacgc tttcgccgcc ccaggaatta gcattgtcga gtcacgccgg atgtcccagg
     3961 caatcagggc ggggtaatga acttcgacaa tagccgagga cgggctgtcg gcatcatcgg
     4021 aaaggacagc gcatgccatt ggtactcgac gcctttcgca acgcgcttgc tgccttgcga
     4081 gcggtccagg ccaagagcga ggatgcggct ttcatggcaa cattggacac ggtaacgcgc
     4141 gaagccattc gatccggcgc gatccagcat ttcgagttca cgttcgaact cgcctggaag
     4201 tttatgcggc gcaggcttga agcggatatc ggacgatcca gcgtcgatgg aataccaagg
     4261 cgcgatctct ttcggatcgg cgccgagaac ggcctgatcg acgatgtcga ggcttggttt
     4321 cagtaccatc ttgcgcgtaa tcggacgtcg catacctacg atccctcgat cgtggccagc
     4381 ctatatgctc gcagcctgga cttcgccaat gatgcggtcg cattgctggc ggttcttgag
     4441 gcccggaatg tttgatattt cgccgcgcga tctggccatc gttcaaacca tcctgcgcaa
     4501 gcatgtgccg gatcacgaag tctgggcctt cggttcgcgt acgaaaggca ccgcacggcg
     4561 ctactccgac ctcgatcttg cgatcatcac cgcaaccccg ctgtcgtacc agatccgggg
     4621 tgatatggaa gcggatcttt cggaatccga cctgccgttc cgggtggata tcctggattg
     4681 ggcgacgacg caggaaaact tccggcgcat cgtcgagcag gatcgggtcg tggtgcagcc
     4741 tggaaagcaa acatgagttc gccgaacggg ggatcagcct catgctgaac ccccacacgg
     4801 ggtcagacca ccgggctggc gccgccatcc atgttgatcg ccgttccggt cgtgaatccg
     4861 gcatgatccg aacacaggaa gcaggccatc gccgcgaatt cctcggcttt gcccatccgc
     4921 ccgactggta tccccgcgcc ggccttggcg acataagcct cgaacggctc gttgctgccc
     4981 tcggtcttgt agcgccgggc gatctggtcc gacacgatct gccccaccag catgccgttg
     5041 accaggatgc cgtgcggcgc gccctcggct gacatcacct tcatcagcgc catgccggcc
     5101 gcgcgcgata ccgccgtcgg cgccccgttc gcccgcggcg ccttcgcccc ggtgttcagc
     5161 acgttgatga tccggcccca tttgcgcgcc ttcatccctg gccagcacaa ccgggccagg
     5221 cggatcgcgg cgaacagctt caggtccaga tcctcttgcc agatgtcgtc gctgatctgg
     5281 tcggacggca tcgcccgcga catcccggcg ttgttgatca ggatgtcaac ctggccgaac
     5341 gtgttgatca ccgcctcgaa cgttttcgag atgtcggcag ccttcgacac gtcgcactgg
     5401 aacgttgcag ccttcccgga cgccccttcg gataccgtcg cccgagccgc ggccagtcca
     5461 tcgggactgc gggcgagaat ggcgacattg gcgccggagg ccgcgaattc ctttgccata
     5521 gccagtccga ggcccttgct ggacccggtg atgatcgcgg tacggccgtc gaggcgtacg
     5581 tccatcggtt ttctccccct gtcgaaaatc ctactttgcg gaagataccc gaaccgtgca
     5641 acgcgtgttg ccgaacacgg agttggctgc cttatgtcgt ccttcgaatt tgtctcggcc
     5701 ttgctgctat tggcggccat cctggggatc gtgaaccacc tttacgtgca tctgccgcga
     5761 accataggtg tgatggtcgg atcgttgatc ctttccatca tcattatcct gatcgaccgc
     5821 tcgatcgatg tcgtggaact gcgggagtgg tggcaacaac tggtcgtttc gaccgacttg
     5881 ccgcgcgtgt tcctggatgg gctgctggcg ttcatgctgt tcgccggcag cctccatgtc
     5941 gatatggaca gcttgcgggc gcacaagtgg accgtgctgg tattggcaac ggtcggcgta
     6001 gtgatggcga cggccctgta cggtttcggc atctggttta tcttccgcga caccgttcca
     6061 ctggcttggt gcttcgccct gggtgcattg ctcgcaccca ccgatccaat cgcggtcggc
     6121 ggcctgttga agcaagccgg cctcccgccc gggctgttgg ctgtcgtgaa cggcgagagc
     6181 ctgttcaatg acggtgtcgc cgtcgtggtc ttcgccgtta ccttagaatg ggccaatggc
     6241 catgtcgcca cacccgccac gatggcggcc gacctgctga tcgaggccgg cggcgggatc
     6301 gtcttgggct tggcgacagg ctacctcgcg tatcgggcgc tgcgcctgat cgatgaaccg
     6361 gggctggaac tgacgatcac cctggccctg gtcaccgtca cctatagcgt ggcgtcggcg
     6421 ctgcatgttt cggggccgct ggctgttgtc gtcgcgggcc tgctgaccgg ccaccgatcg
     6481 acccggttcg ccatgaccga ccgctcccgc agggaggtca tattgttctg ggacatgatg
     6541 gacgaattgc tgaacaccgt actgttcctg ttgatgggat tcgcgctgct gtcggtggac
     6601 atcgacaccc acctgctgct gggtgctgcc ggcggtatcg gcctctcgct ggtcaccagg
     6661 ctgatcag
//
LOCUS       DASWQN010000092         6024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1330058, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000092 DASWQN010000000
VERSION     DASWQN010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330058"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(730..1749)
                     /locus_tag="VGC82_01485"
     CDS             complement(730..1749)
                     /locus_tag="VGC82_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0421978.1"
                     /translation="MNTASMTQPLRPLLDVRGLRKYFPIANGLLRRVSGQVRAVDDVS
                     FTVNEGETLGLVGESGCGKTTVSRCILRAVDPTGGQVLYRTRDNRIVDLAPMSRQDLA
                     PLRKEIQMIFQDPFGSLNPRMTLLDNVGEPLLVNGVRSRRERTERVGELLRLVGLRPE
                     FMHRFPHAFSGGQRQRIGIARALATNPRLVVADEPVSALDVSVQAQVLNLLLELQASL
                     RLTFLFVAHDLSVVRHISDRVAVMYVGQLVELAPTAAIFERPYHPYTAALLLAVPVPD
                     PRVRNDVGVLRGEVPNPAAAPSGCYFHPRCGFAEDRCRVEAPALQEIAPGRFARCHFA
                     GQLEL"
     gene            complement(1746..2786)
                     /locus_tag="VGC82_01490"
     CDS             complement(1746..2786)
                     /locus_tag="VGC82_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0421979.1"
                     /translation="MPPIHTATDPLLRVRDLRVSFKLDEGTVRAVDGLSFDVFPGQVV
                     GIVGESGCGKSVTVKAILQLIEPPGQITSGQIRYRQDNETIDLVRLPPRGSAMRALRG
                     AEIALIPQEPMAAFSPVHTVGDQIIEAIRLHEKSNGRPVSRRAARARTIELFRDVGIS
                     MPEQRVEAYSWQLSGGLRQRAMIAMALSCRPRLLIADEPTTAIDVTTQAQILALLRDL
                     QGKYGTSIIFITHDLGVIAQMANHVVVMYLGRVMEEGPVDEIFHAPKHPYTRALLRSM
                     PSMHGETRVALPIISGTLPHPFNRPPGCPFHPRCPEAMANRCNLRVPVPREIEANRVV
                     SCFLYHDEAEAQ"
     gene            complement(2761..3879)
                     /locus_tag="VGC82_01495"
     CDS             complement(2761..3879)
                     /locus_tag="VGC82_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0421980.1"
                     /translation="MSDAIAEIGAVTEERIAIAPNWKLVWWRFRRHHLAMFSAVVLAG
                     FYLVVLCPDFFSTQSPEQTDARQAFIPVQTLHLFDEGGWNPWVPAIVGKRNPVTLRME
                     WIADPARRVRVGFFAPGPSYRIFGLFPASTHLIAAATPGQRIFLLGSDRLGRDQWSRI
                     MHGTQTSMTVGLVAVTLSVIFGVVLGGISGYFGGMADQIIQRLIELLQSVPTIPIWLA
                     LSAALPRDWTPIQVFFAITVILSLIGWTTLGREVRGRFLALREEDFVLAARLAGCGRM
                     RIILRHMMPTFLSHIIATSSLAIPVMIINETSLSFLGLGIRPPAISLGVLLQEAQNIQ
                     TLALAPWLLMPGVVVIVAVLAFNLVGDGLRDASDPYSH"
     gene            complement(3879..4865)
                     /locus_tag="VGC82_01500"
     CDS             complement(3879..4865)
                     /locus_tag="VGC82_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0421981.1"
                     /translation="MIRYIAQRLLLLPFLMLVYSFVIFVIIKAPPGDFLTAYVANLSA
                     SGTSITAEMIQALRHDYGLDQPFIVQYFHWMVNLLHGNMGLSLEYQRPNIDLISEQLV
                     LTIALALMSFVLTWVIAVPAGIYSATHQRSVIDHIFTVINYIGVATPNFMLALILMWL
                     AFAYFGVSVTGLFSPDYVDAPWSFARLLDLLAHIWLPAIVLGIAGTARLTRIMRANLL
                     DELNKPYVMTARAKGMREWRLVLRYPVRLALNPLISTIGWYLPALFSGSLIVATVMNL
                     PNIGPLLLRALINQDMYLAGGILLIYSFLTIVGTLLSDVLLALIDPRIRMEN"
     gene            complement(4884..>6024)
                     /locus_tag="VGC82_01505"
     CDS             complement(4884..>6024)
                     /locus_tag="VGC82_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0421982.1"
                     /translation="AISRVSDPVFTLRRNPYFIGVDPAGNQLPYINEVRFTYFADVQA
                     LNLQAIAGNFDMQARHIQMTNYPVFKEQEKNGKYRVITWPTFGGSDAVIAFNQTYTAD
                     PAIGKLMATKAFRVALSLAINRDEIRESVFLGLGENRQAVPAPWHPNFPGQEWAVKYT
                     EFNPGEANKMLDALGLTKRDPQGIRLMDNGKPATIEISVVPAFGAWPDVAQLVAKDWE
                     SVGVRTVVQIRERALHFSMRDANQLMSEIWNEDSTGFPFTGNSKIDPRNSPILTLGPL
                     FTTWINSQGKEGVEPTPPIKRIMELVDTARRVGPDQQAKAAQELFRIWADEVYEIGTV
                     GLTPMIQGVVVVNSKFHNVPSTIGNDWPLRTPGNARTEQFFFASH"
BASE COUNT         1246 a         1837 c         1845 g         1096 t
ORIGIN      
        1 gtggcggcgg gggaagtgtc ttcaacgctt cgtcgttgat tgccgaatag ccctttccgg
       61 caatcttctc ggttgccttg cctgcgcgtg tgttgaagat cttcaagccc gtggcttcgt
      121 cggttctgat tggcgccgtc atacagggtt aactcccacg atcgggttga acacgttcgg
      181 gcgggctcgc cggcggccat cgtgcggcac gataatcatc ggcccatgac tctacaaccg
      241 tcgcctctca gccatcatca tcccatcaat cgatcggcac caggcggtag ctgggttcgg
      301 ctgatccgtc accatgacgc ttaagagcga aatccgtgct gacagacaca aggcttccat
      361 cctggccctt gttttcgcga gcgtctttat ccggtcagtg atcccacatg gccggatgat
      421 gttctagcca ggtggcagcg cctcggcgag cttgacgacc tcgataccgg cggtccgcac
      481 aggtcgcttt tgtttcggga tcgcgcaaca cggccacgac cagactacga gcaacccggc
      541 ggtgccaatc atattggcag ggcaaagctc agagtctgat ccagggtaaa atacgccagg
      601 acggtgcggc aaagccgtcc agatggcgcc tggtggcaga caaaacccac ggcggtggcg
      661 cgacctgtct cttggaagtc attccgtcag ataggacgga ttcgattgcg atggcctgct
      721 acatatcaac taaagctcaa gttggcccgc gaagtggcag cgcgcgaagc ggcctggggc
      781 gatctcttgc aacgcgggcg cttccactcg gcagcgatcc tcggcgaacc cgcaccttgg
      841 atgaaaatag catcctgatg gggccgccgc cggatttggc acttcgcccc gaagaacgcc
      901 gacgtcattg cgcactcgtg gatcgggcac cggaacggcg agcaggagtg ccgcggtgta
      961 cgggtggtaa gggcgttcga aaatggccgc ggtcggggcc agttcgacca gttgaccgac
     1021 atacatgacc gcgacccggt cggagatgtg acgcaccacc gacaggtcgt gcgcgacgaa
     1081 cagaaatgtc agccgcaacg acgcctgaag ttcaagcaac aggttcagca cctgtgcctg
     1141 aaccgacacg tcgagggccg acaccggctc gtccgccacg accagcctgg gattggtcgc
     1201 cagggcacgc gcgatgccga tccgctggcg ttggccgccg ctgaaggcat gcggaaaccg
     1261 atgcatgaat tcagggcgca gtcccacaag ccgtagcagt tcgccgaccc gttccgtgcg
     1321 ctccctgcgg ctgcgaacgc cattgaccag cagcggttcg ccgacattgt ccagcagggt
     1381 catgcggggg ttcagggacc cgaatggatc ctgaaagatc atctggattt cttttcgcag
     1441 cggcgccagg tcctgacgcg acatcggcgc gagatccacg atccggttat cccgggtgcg
     1501 atacagcact tgcccgccgg tcggatcgac cgcccgcagg atacagcgcg aaaccgtcgt
     1561 cttgccgcag ccgctttcgc cgacgagacc gagcgtctcg ccctcgttga cggtgaagct
     1621 gacgtcgtct acggctcgca cctggccgga cacgcgccgg agcaatccgt tggcgatcgg
     1681 aaagtatttc cgcaagccgc gaacgtcgag cagcggtcgc aacggctggg tcatggatgc
     1741 cgtgttcatt gcgcctccgc ctcatcgtga taaaggaagc agcttacgac ccggttggcc
     1801 tcgatctcgc gcggaacagg cacgcgcagg ttgcatcggt tcgccatcgc ttcgggacag
     1861 cggggatgaa acggacagcc gggggggcga ttgaacggat gcggcaatgt gccgctgatg
     1921 atcggcagcg cgacccgggt ttccccatgc atgctcggca tcgatcggag cagcgccctt
     1981 gtgtagggat gttttggcgc atggaagatc tcgtccaccg gcccctcctc catgacccgg
     2041 ccgaggtaca tcaccacgac atggttcgcc atctgcgcga tcactccaag gtcatgcgtg
     2101 atgaaaatga tagaggttcc gtactttcct tgcagatcac gcagcaatgc cagaatttgc
     2161 gcctgggtgg tgacatcgat cgccgtcgtt ggttcgtcgg caatcagcaa acgcggccgg
     2221 caggacagtg ccatcgcaat catcgcgcgc tggcgcagtc cgccggacaa ttgccacgag
     2281 taagcctcga ctctctgttc cggcatggaa ataccgacgt cgcggaacaa ctcgatcgtc
     2341 ctggcgcgcg ccgctcgccg cgaaaccggc cgtccatttg atttctcgtg gagcctgatc
     2401 gcctcgatga tctggtcgcc aaccgtgtgt accgggctga acgcggccat cggttcctgc
     2461 gggatgagcg cgatctcggc gccacgcagc gcccgcatcg cggacccgcg cggcggcaaa
     2521 cgaacaaggt cgatcgtctc gttgtcttgc cgatagcgga tctgcccgct ggtgatctgg
     2581 cctggcggct cgatcaattg caggatggct ttcacggtga cgctcttgcc gcaaccgctt
     2641 tcgccgacga tgcccaccac ctggccggga aagacgtcga agctcaaccc gtcgaccgcg
     2701 cggaccgtgc cctcgtccag tttgaacgat acgcgcaggt cgcgaacccg tagcagggga
     2761 tcagtggctg tatggatcgg aggcatcgcg aagtccgtca cccaccaggt tgaacgccag
     2821 cacggccacg atcaccacga cgccgggcat gagcagccac ggggccagtg cgagggtctg
     2881 gatattctgt gcctcctgca gcagcacgcc caaactgatc gccggaggcc ggatgccaag
     2941 tcccaggaag gacagcgagg tttcattgat gatcatcacg ggaattgcca gcgaactggt
     3001 ggcaatgatg tggctgagga aggtcggcat catatggcgc aggatgatcc gcatccggcc
     3061 gcagcccgcc agccgcgcgg ccagcacgaa atcctcctcg cgcagcgcca ggaagcggcc
     3121 tcggacttcg cggcccaaag tggtccagcc gatcagcgac agaatgaccg taatggcaaa
     3181 gaatacctgg atcggcgtcc agtcacgcgg cagagcagcg gacagcgcga gccagatggg
     3241 aatggtcggc accgattgca gcagttcgat cagccgctgg attatctggt ccgccatgcc
     3301 accgaaatag ccggagatcc cgccgagaac tacgccgaaa atgacgctga gggtgaccgc
     3361 caccaggccc accgtcatcg aagtctgcgt accgtgcatg atccgcgacc attgatcgcg
     3421 ccccagccgg tccgaaccaa gcagaaagat ccgctgcccc ggcgtggcgg cggcgatcag
     3481 atgggtgctg gcgggaaaca ggccaaaaat ccgataggac ggcccggggg cgaagaaacc
     3541 gacccgcacc ctgcgcgccg gatcggcaat ccattccatg cgcaacgtga ccggattgcg
     3601 cttgccgacg atcgccggga cccagggatt ccatccgccc tcatcgaaca gatgcagtgt
     3661 ctgcaccggt atgaaggcct ggcgcgcatc cgtttgttcg ggactttgcg tgctgaaaaa
     3721 atcgggacac aacaccacga ggtaaaaccc ggccaggacg acggcgctga acatagccag
     3781 gtggtgcctg cggaaccgcc accacaccag cttccagttc ggcgcgatcg cgatgcgttc
     3841 ctcggtcacc gcgccaattt cggcgatcgc gtccgacatc agttttccat ccggatgcgc
     3901 gggtcgatca atgccaatag aacatcggac aacagagtac cgacgatggt caggaacgag
     3961 tagatcagca aaatgccgcc cgcgagatac atgtcctggt tgatcaacgc gcgcaacagc
     4021 aacggaccaa tattcggcag gttcatcacc gtcgcgacga tcaggctgcc agagaacaac
     4081 gccggcaaat accagccgat cgtgctgatc aggggattga gcgccaggcg gaccgggtag
     4141 cgcagcacca accgccactc tcgcatgccc ttcgctcgcg cggtcatcac gtatggcttg
     4201 ttcagttcgt ccagcaagtt cgcccgcatg atcctggtca gccgcgcggt ccccgctatg
     4261 ccaagcacga tggccggcag ccagatatgc gcgagcaaat ccagcagtcg cgcaaaactc
     4321 cagggcgcat cgacgtagtc aggggagaac aggccggtga cgctgacacc gaaataagcg
     4381 aatgcgagcc acatcaggat cagcgccagc atgaaattcg gcgtcgccac gccgatgtaa
     4441 ttgatcacgg tgaagatatg gtcgatgacc gaacgctgat gcgtggcgga atagatgccc
     4501 gccggaaccg cgatcaccca ggtaaggacg aaggacatca gcgcaagggc gatggtcaat
     4561 acgagctgct cgctgatcag gtcgatgttg ggccgctgat actccaacga caggcccatg
     4621 ttgccatgca ggagattcac catccagtgg aagtactgga cgatgaacgg ctgatcgagc
     4681 ccataatcgt gccgcagcgc ctgtatcatc tcggcggtga tggaagtgcc ggatgccgac
     4741 aggttggcga cgtacgcggt taggaagtcc cctggcgggg ccttgatgat gacgaagatg
     4801 acgaacgagt aaacaagcat caggaacggc agcagaagca gtcgttgcgc gatgtagcgg
     4861 atcatgctcg aacgtcgggc gcgctagtga gaggcaaaga agaactgttc ggtgcgggcg
     4921 tttccgggcg tgcgcaacgg ccagtcgttg ccgatcgtgg acggcacgtt gtggaatttg
     4981 gaattgacga ccacgacgcc ctggatcatc ggcgtcaagc cgacggtgcc gatttcatag
     5041 acctcgtcag cccaaatacg aaacagttct tgcgcggcct tggcctgctg gtcgggacca
     5101 acccttcggg ccgtgtccac gagctccatg atccgtttga tcggaggtgt cggctcaaca
     5161 ccttctttcc cttgagaatt gatccaggtg gtaaacaacg gaccaagcgt aaggataggg
     5221 gaattgcgcg gatcgatctt ggagttgcca gtgaagggaa aacccgtcga gtcctcgttc
     5281 cagatttcgc tcatgagttg attggcgtcg cgcatgctga agtgcaacgc ccgttcgcga
     5341 atctgaacga ccgtcctgac cccgaccgac tcccagtcct tggcgacgag ctgggctacg
     5401 tccggccatg cgccgaaggc cgggacgacg ctgatttcaa ttgtcgccgg cttgccgttg
     5461 tccatcagcc ggataccctg cgggtcgcgc ttcgtcaggc caagcgcatc caacatcttg
     5521 ttggcttcgc cgggattgaa ttcggtgtat ttcaccgccc actcctggcc gggaaaattc
     5581 gggtgccaag gtgccggaac ggcctgacgg ttttccccca aacccagaaa caccgattcc
     5641 cggatctcat cgcggttgat cgcgagcgac aacgcgaccc ggaatgcctt cgtcgccatc
     5701 agctttccga tcgccgggtc ggcggtatag gtctggttga acgcgatcac ggcatccgaa
     5761 ccaccgaacg tcggccaggt gatgacacgg tacttgccgt tcttttcctg ctccttgaac
     5821 accggatagt tcgtcatctg gatatgccgc gcctgcatgt cgaaattgcc tgcgatagcc
     5881 tgcaggttca gcgcttgtac atcggcgaag taggtaaacc tgacctcatt gatgtacggc
     5941 agttggttgc cggcgggatc aaccccgata aagtacgggt tgcgccgcag cgtgaacacc
     6001 ggatcgctga cgcggctgat cgcg
//
LOCUS       DASWQN010000093         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_142629, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000093 DASWQN010000000
VERSION     DASWQN010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142629"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..602)
                     /locus_tag="VGC82_01510"
     CDS             complement(75..602)
                     /locus_tag="VGC82_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0421983.1"
                     /translation="MRWRHKAAIWLAASVGFVFGLLTASSALMPAGTRPSAQVAAAGS
                     RWTWYLGVACAVLVVACAGVAGWQFSEASQRTEVARSLTGGDPARAPVLMIRYGCGGC
                     HTIPGVPGADGQVGPNLSGLRARVFVGGVARNDAANLIHWIVNPRDLSPRTAMPSTGI
                     SPAEARDVAAFLYTR"
     gene            complement(586..1518)
                     /locus_tag="VGC82_01515"
     CDS             complement(586..1518)
                     /locus_tag="VGC82_01515"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY0421984.1"
                     /translation="MKRFGLVLLLTPAPACAHAPALAHAPALAHAPAFAHEDIGNSLW
                     TFDPWITVPLLLSAALYATGALRLRRRGVTGRRTRFWRGLAYTAGWCSLAAALVSPLH
                     WFGEQLFTAHMIEHEVVMTIAAPLLVLARPGGVLLWAFRTNTRREVGRLARGSSARRC
                     WEVLTRPLTATVLHGMAIWTWHVPLLFDAAVADIVVHRLQHLTFFATAILFWWAMLWR
                     SNPAVAAGEIFITMIHTGILGALMTFAPHVLYGTQSINAEYWGLTPLEDQQLAGLVMW
                     IPVGTIYAGAAIGLLGRWVKRSGKSWKVADALAP"
     gene            complement(1515..1853)
                     /locus_tag="VGC82_01520"
     CDS             complement(1515..1853)
                     /locus_tag="VGC82_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421985.1"
                     /translation="MNIASRPGLAACAGLVAAPLLWAVNMQASQILPYFDCGKSIRIT
                     AVLSLFSLLLALGSGWVSWRARRFLTNSNDSARPLRFVAMLGCLAAVTFAFALLLQAA
                     AGLTLTGCER"
     gene            complement(1895..>2079)
                     /locus_tag="VGC82_01525"
     CDS             complement(1895..>2079)
                     /locus_tag="VGC82_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C oxidase subunit III"
                     /protein_id="HEY0421986.1"
                     /translation="SNAYGSIVWTMLGLHSTHLITDLADTLVLAVLMFTRHGRNPRRF
                     GDLQDNAMYWNFVVLT"
BASE COUNT          448 a          642 c          691 g          298 t
ORIGIN      
        1 tgcgcggcca tgatactatt ttgatgcgtg gtcatgacgc tacaaccaag cacggccgtg
       61 acactgcaac ccatctaacg cgtatagagg aacgcggcta cgtcgcgtgc ttcggctggt
      121 gagatgccgg ttgagggcat cgcggttcga ggcgacaaat cgcgtgggtt gacgatccag
      181 tggattagat ttgccgcgtc gttgcgggcg acgccgccga cgaagacgcg ggcgcgcaat
      241 ccggagagat tcgggccgac ctgaccgtcg gcaccgggaa cgccagggat cgtgtgacag
      301 ccgccgcagc catagcggat catcaggact ggtgcccggg cgggatcgcc gccggtcagg
      361 gaacgcgcga cttcggtcct ctggcttgcc tcgctgaact gccatccggc aaccccggcg
      421 caggcaacca cgagaacggc gcacgcaaca cccagatacc aggtccatcg agacccggcc
      481 gcggcgactt gtgctgacgg ccgggtgccg gccggcatta gggcggagct tgcggtgaga
      541 aggccgaaca cgaagcctac cgacgcagcc agccagatcg ccgctttatg gcgccaacgc
      601 atccgccacc ttccatgact tgccggagcg cttcacccac cggcccagca agccgatcgc
      661 cgcacccgcg tagatcgtgc caaccggtat ccacatcaca agcccagcca gttgctggtc
      721 ctccagcggc gtaagtcccc aatactcggc gttgatcgat tgcgtgccgt agagcacatg
      781 cggcgcaaag gtcatgagcg cccccaggat tccggtatgg atcatggtga tgaaaatctc
      841 tcccgccgcg acggccggat tacttcgcca gagcatcgcc caccagaaca agatggcggt
      901 cgcgaagaag gtcagatgct gcagccggtg cacgacgatg tctgcgaccg ccgcgtcgaa
      961 cagaagtggc acatgccagg tccagatcgc catgccgtgc aaaaccgtcg cggtcagagg
     1021 ccgggtcagg acctcccagc accggcgtgc cgaggagccg cgcgccaagc ggcctacttc
     1081 acggcgggtg ttggtgcgga atgcccaaag cagcacgccg ccgggccgag ccagcaccag
     1141 cagcggtgcg gcaatggtca tgacgacttc atgctcgatc atgtgggcag tgaaaagctg
     1201 ttccccgaac cagtgcagcg gcgagacgag cgcggccgcg agtgaacacc agccggcggt
     1261 ataggccagg cctcgccaga accgtgtacg ccgccccgtg acgcctcgcc ggcggagtct
     1321 gagtgcgccg gttgcgtaca acgcggcgga gagcaggaga ggaacggtta tccaggggtc
     1381 gaacgtccac aaggaattcc cgatatcctc gtgggcaaag gcgggagcgt gggccaaggc
     1441 gggggcgtgg gccaaggcgg gggcatgcgc acaggcgggc gccggagtca gaagcaaaac
     1501 caggccaaag cgcttcatcg ttcgcatccg gtgagggtca aacccgccgc cgcctgcagc
     1561 agtagcgcga aagcaaaggt cacggcggcg aggcagccca gcatcgcaac gaaccggagg
     1621 ggacgcgccg agtcgttgga attggtcaga aaccgcctgg cgcgccacga aacccagcca
     1681 ctgcccaggg ccagtagaag cgagaacagg gacaggaccg cggtaatccg aatcgacttg
     1741 ccgcagtcga agtaaggcaa gatctggctg gcctgcatat ttaccgccca aagcagtgga
     1801 gcggccacca gcccggcgca agcggcgagt ccggggcgag atgcgatgtt cattgcagac
     1861 gcggcagaaa atagatgcag aaatagaccg gcagtcaggt caggacgacg aagttccagt
     1921 acatggcgtt gtcttgcaga tcgccgaagc ggcgcgggtt tcggccatgg cgcgtgaaca
     1981 tcagcacggc cagaaccagc gtgtcggcaa gatcagtgat aaggtgcgtg ctgtgcagac
     2041 caagcattgt ccagacgatc gacccatagg cgttgctgt
//
LOCUS       DASWQN010000094         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2386012, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000094 DASWQN010000000
VERSION     DASWQN010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2386012"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1866)
                     /locus_tag="VGC82_01530"
     CDS             complement(202..1866)
                     /locus_tag="VGC82_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY0421987.1"
                     /translation="MNDGRNISEIDVQIMWNRLLSVVEEQGQTLVRTAFSTSAREAGD
                     ISAGVFDLRGRMLAQAVTGTPGHINTMAASVGHFLDVFPVADMQEGDVYITNDPWKGT
                     GHLYDLVVVSPTFLDGRIVALFACTSHLVDMGGVGQTPEGRQIWHEGLFIPLLRLVRA
                     GTMNEDLLAMIRANVREPVQVIGDVYALIACNEIGSRRLVSMMREFGLLDLDRLGEEI
                     INRSRRAMSEAIGRLPKGTWTNTMRVDGYEAPLDLVASVTIGADTIHVDFTGTSGMST
                     YAINCPLCYTEAYTTFGINCVIAPTIPNNAGTLAAVKVTAPAGTIVSATHPAAVYARS
                     SIGHMMPDVVYGALEQAIPGRVPAEGTSNLWSLKMVAGHGLTGVGDQAGTQFMVMSFH
                     SGGAGARPHQDGLSATPFPSGVRNVPVEATEAITPLVIWRKELRQDSGGPGRYRGGLG
                     QVMEVGSREDAAFGLFAGFERVKYPARGRNGGGSGQCGSVSLKSGAALKPKGLQVVPS
                     GERLLIEMPGGGGMGPASERDPESVKRDVRLGYLSVEAAKRDYGVG"
     gene            complement(1863..>2206)
                     /locus_tag="VGC82_01535"
     CDS             complement(1863..>2206)
                     /locus_tag="VGC82_01535"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY0421988.1"
                     /translation="TTYARLFGRIIPGLEIEAVTWTLALSQPYELPAAVLPVATRTPA
                     KPSSIRRMVEAAGGQIVDAPVYTRSALRPGARLSGPAAIVEDGTTSVVPVGFRAHIAA
                     GGEIVIEEGSA"
BASE COUNT          399 a          737 c          683 g          387 t
ORIGIN      
        1 aaggccgagg cgttgcagcc agaacggacc gtcgcaagct attagcgact agcgtgccag
       61 cgagggggtc gcggtgactg gaaacagggt tcttttgtgg ctgcaacgaa agtcgtgggt
      121 ggcccaggcc aagcccggcc gccgtcactt ggacgtcagg cggcaatctg gctcgcacac
      181 ttccagtcaa cgtaaagcgc ttcaacctac tccataatcc cgcttcgctg cctcgaccga
      241 cagatatcct agccgcacgt cccgtttgac ggattcggga tcacgctccg atgccggacc
      301 catcccgccg ccgccgggca tttcgatcag cagccgctcg cccgagggaa ccacctgcaa
      361 tcccttcggc ttcaacgccg caccggattt tagcgatacc gaaccacact ggccggaacc
      421 gccgccgtta cgcccccggg cgggatactt gacgcgttcg aaaccggcga agaggccgaa
      481 ggctgcatcc tcgcgggaac cgacttccat cacctggccc aggccgccgc gatagcgtcc
      541 tggaccgccg gaatcctgcc gcaactcctt gcgccagatc accagcggcg tgatcgcttc
      601 tgtcgcttcc accgggacgt tgcgcactcc ggaaggaaac ggggtcgccg acagcccatc
      661 ctggtggggc ctggcgccgg cgccgccgga gtggaagctc atgaccatga actgggtgcc
      721 ggcctgatcg ccgacccctg tcaggccgtg accggcgacc atcttcaggc tccaaaggtt
      781 ggatgttccc tcggctggca cgcgtcctgg aatcgcttgc tccagcgcgc cgtacacgac
      841 gtccggcatc atgtgcccga tcgaggaacg ggcatagacc gccgccggat gggtcgcact
      901 gacgatcgtc cccgccggtg ccgtcacttt caccgcggcc aaagtaccgg cgttgtttgg
      961 aatggtcggt gcgatgacgc agttgatgcc gaaggtcgta taggcttccg tatagcagag
     1021 cgggcagttg atcgcgtagg tggacatccc ggatgtgccg gtgaaatcga catggatggt
     1081 gtccgcgccg atggtaacgg atgcaaccag atcgagcggc gcctcatagc catcgacccg
     1141 catcgtgttc gtccatgtgc ctttcggcaa ccggccaatc gcctcgctca tggcccggcg
     1201 gctgcggttg atgatctcct cgccgagcct atcgaggtcg agcaggccga attcgcgcat
     1261 catcgagacg aggcggcggc taccgatttc gttgcacgcg atcagcgcgt aaacgtcgcc
     1321 gatcacctgc accggttcgc gcacattggc tcggatcatc gccagcagat cctcgttcat
     1381 cgtcccggcg cgcaccaggc gcagcagcgg tatgaacagg ccttcgtgcc agatctgccg
     1441 gccttcgggg gtctggccga caccgcccat atcgaccaga tgcgacgtgc atgcgaacag
     1501 cgccacgatc cgcccgtcca ggaaggtcgg cgacaccacc acaaggtcgt aaagatggcc
     1561 ggtgcctttc cacgggtcgt tggtgatata gacatcgcct tcctgcatat cggcgaccgg
     1621 aaacacgtcc aggaaatgcc ccaccgatgc ggccatcgtg ttgatgtgcc cgggcgttcc
     1681 ggtgacagcc tgggccagca tccgtccacg caggtcgaac acgccggcgg agatgtcgcc
     1741 ggcctcccgc gccgaggtgc tgaaggccgt ccgtaccagg gtctgtccct gctcctcgac
     1801 gaccgacagc agccggttcc acatgatctg cacgtcgatt tccgagatat tgcggccgtc
     1861 gttcatgctg agccttcctc gatgacgatt tcgccgccgg cggcgatgtg ggcgcggaac
     1921 ccgactggaa caactgacgt ggttccgtcc tcgacgatcg cggcggggcc ggacagcctg
     1981 gcgccgggac gcaatgccga gcgcgtatag acaggcgcat caacgatctg gccgccggcg
     2041 gcttccacca tccgccggat tgacgacggc ttggctgggg tccgtgtggc caccggcaaa
     2101 accgccgccg gcaactcgta aggctgcgag agcgccaatg tccacgtgac tgcctcgatc
     2161 tccagtccgg gaatgatacg gccgaacaac ctggcgtagg tggtct
//
LOCUS       DASWQN010000095         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_275002, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000095 DASWQN010000000
VERSION     DASWQN010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275002"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..797)
                     /locus_tag="VGC82_01540"
                     /pseudo
     CDS             complement(264..797)
                     /locus_tag="VGC82_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(810..1676)
                     /locus_tag="VGC82_01545"
     CDS             complement(810..1676)
                     /locus_tag="VGC82_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0421989.1"
                     /translation="MLTAAAITADSQTAAAILARLDRLPATRHIWAMVTLLSLGGMFE
                     FYDLFMTGYVTPGLVKAGLLTGVSIGMFSGPALFVASTFFGLFIGTFVFGFVADKYGR
                     RAIFTFSMLAYCAATLVMAFQTTGFGVCLWRMIAGIGIGVELVTIDTYVAELVPKHMR
                     GQAFAFNQCVQFSVVPVVAFLSYVLVPQAPLGWDGWRWVVAIGAIGALFVWFLRRAIP
                     ESLRWLLTHGRLAEAEAITSQIEAHVLADRQGAALPPPAAHEVEDPSARGTFGEMWQP
                     PLRSRTIMLMVF"
     gene            complement(1816..>2171)
                     /locus_tag="VGC82_01550"
     CDS             complement(1816..>2171)
                     /locus_tag="VGC82_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0421990.1"
                     /translation="TRAPNDPVEAWVVTSLERPLMREGRRPDRAAGDAERKGDDVLRL
                     AGVVTGPFGDRAIFVLPGVAKPVVANVGGQLGDFVVRSIEPGRVVVEANGVARAYRPM
                     YSGSNRKPMDALKRS"
BASE COUNT          458 a          685 c          681 g          347 t
ORIGIN      
        1 gatcggtttg atggctacat cccggcctgc ggctaatttc gcaattgaac cgtaagccgc
       61 cgtgacgagg ctctttgcta cgaacattct gcggcaccct ggttcgctgg cagaggagcg
      121 gaacagctac gtgataatgc ggcgaagcgc tggccgcatg cacccgacag tatcccgcgt
      181 tgggagtaac cggcaccaga tcgctatagc atcattgcag ttcctatgtc gtgtcgtggt
      241 ctagactact cctgccccgc agttcaacgc gaaatccgct ccaattccag attgctcgtc
      301 cgcggcccaa agatcccgat cgacagaacc accatcagca tggcgaaggc gatcagcccg
      361 aacacccccg tggtgccgtt gaacgcggca aagaagaacc cgatgatgaa gcttgcgaac
      421 accgtgctga tgcggctgaa cgcgtaggta aacccgacgg cccgggcgcg cacgcgggtg
      481 ggaaacagct ccgattggta gctgtggaac gagtaggaca gcacattgtt cgatagcgtg
      541 atcagcacgc ccaacaggat cagcatcggc atggtggact ggtaggagaa cagcacgccg
      601 aataccgcga tgcagatcgc cgaccccacc agctgccatt tgcgttcgaa cctgtcggcg
      661 aagctcatgg ccagaagcgg gccgaacggg ttggcgcagg cgacgatgaa cgcatactgc
      721 aggctggcgc ccaggttgat gccgacctgc ttggcgatta gcgtcggcac ccaggcggcg
      781 aacccgtaga aaccaaaggt ctggaagaac tagaacacca tcagcatgat ggtccgggat
      841 cggaggggcg gctgccacat ctcgccgaag gttccgcggg cggatggatc ctcgacttca
      901 tgggcggcgg gcggcggcaa ggcggcgcct tggcggtcgg cgaggacatg agcctcgatc
      961 tggctcgtga tcgcttcggc ttcggccagg cggccgtgcg tcagcaacca gcgcaggctt
     1021 tccggtatgg cgcggcggag gaaccacacg aacagcgcgc cgatcgcgcc gatcgccacg
     1081 acccagcgcc agccgtccca gcccagcgga gcttgcggca ccaggacgta ggacaggaat
     1141 gcgacgaccg gtaccaccga gaactgcacg cactgattga acgcaaaggc ttggccgcgc
     1201 atgtgcttgg gcacgagttc agcgacgtag gtatcgatgg tcaccagttc gacgccgatg
     1261 ccgatgccgg cgatcatgcg ccataagcag accccgaagc cggtggtttg gaacgccatg
     1321 accaatgtgg cggcgcaata ggccagcatc gagaaggtga agatcgctcg gcggccgtat
     1381 ttgtcggcga cgaagccgaa cacgaatgtg ccgatgaaca ggccaaagaa ggtggaagca
     1441 acaaatagcg ccggacctga gaacatgcca atggacacgc cggtcagcag ccccgccttg
     1501 accagcccgg gagtgacgta gccggtcatg aacaggtcgt aaaactcgaa catgccgcca
     1561 agggatagca gcgtgaccat ggcccagatg tgccgcgtcg ccggcaaccg gtccagccgc
     1621 gccaggatgg cagctgccgt ttggctatcc gctgtaatgg ccgcggccgt caacatgtcg
     1681 ctcctccgct tgttcgccca gccttgcggg gcgatccggc gagcaggaat ctcagacccc
     1741 ggacgtgcca ctaacgctgt cagagcacgg cggggtgcat gcggggcgtc ttatgcccgc
     1801 agatggccgc tgacgttacg aacgcttcag agcgtccatt ggcttgcgat tactcccgga
     1861 gtacatcggc cggtatgcgc gcgccacgcc gttggcctcc accacaaccc ggcccggctc
     1921 gatcgaccgg accacgaagt cgcctaattg tccgccaacg ttggccacga ctggcttggc
     1981 tacgcccggc agcacgaaga tcgcgcggtc gccgaacgga cccgtcacga caccggctaa
     2041 cctaaggaca tcgtcgccct tccgttcggc atcaccagcc gcccgatccg gccgccggcc
     2101 ctcgcgcatc aacgggcgct ccagagaggt tacaacccac gcttcaacgg gatcgtttgg
     2161 tgcacgtgtc g
//
LOCUS       DASWQN010000096         2708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_11204, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000096 DASWQN010000000
VERSION     DASWQN010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11204"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1047
                     /locus_tag="VGC82_01555"
     CDS             178..1047
                     /locus_tag="VGC82_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0421991.1"
                     /translation="MLQLVLFNVTNGLIIGAFYVLMALGLSLILNLSNVINFAHGNFL
                     ALGGYIAYVLTPYVGFWGALIISPVLTAAFGFLIERLMIRRVYTRDPVYSLLLTFGLA
                     FIVQDVCRYIWGPNGLPMSIPSVLSSPLTPDLFFITWYRVFMVAIVIIAVAGLFAFLR
                     FTRVGIRIRAGTLDLDTVAALGVNVGMLRSLNFAVGSLLAGLAGVLAAGQIGLNPNMG
                     DDLLMPSFIAIIVGGVGSLTGTLLGGLLIGVAGALTTVFWPAASEAIIYVIMLVVLVA
                     RPRGLLGEEGMMA"
     gene            1044..2030
                     /locus_tag="VGC82_01560"
     CDS             1044..2030
                     /locus_tag="VGC82_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0421992.1"
                     /translation="MSVTTVAEAPKPAASVRPWLTAAIVWSLLITVPLWLPMLGGYTA
                     LAGRVIVLGLAAMSFNLLLGFTGVMSFGHAAYFGLGAYGAGLTLKFIAASTPLALLAG
                     ILLGGLAGTLFGLLIVRRRGVYFAMVTVAFGQIAFYIAYSWNDLTGGYDGLRGFSRAP
                     LNLGITTIDITNNGTAFYYFLLVVFAIATGLQGLLLASPFGRTRLAIRENERRARFLG
                     IPIERHIWLSFSISCCFTALAGALYALLNNFADPLGLHYSLSGEIVIMTVMGGMRAFW
                     GPLVGAALFVVLQDYISSMTVNWMSFVGLIFVLVVLFFPRGLLGMLQGSSRK"
     gene            2033..>2708
                     /locus_tag="VGC82_01565"
     CDS             2033..>2708
                     /locus_tag="VGC82_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0421993.1"
                     /translation="MLDVRDVTKKFGNLVAVSGVSMQVAPGELRAIIGPNGAGKTTFF
                     NMISGFFAPTAGDIVFDGTAVTKLAVTERVAMGMARTFQITEIFPELTVRENIRIGVE
                     SAQGFRLRFWLGARHKADVDQGIEEALTQTGLTGKADRLVGELSHGDQRAAEIATALT
                     LKPRLLLLDEPTAGMGEHETYAVANLIRRLHRNSAYTIVLIEHDMRVVFNLADKITVL
                     GEGKMLA"
BASE COUNT          438 a          862 c          815 g          593 t
ORIGIN      
        1 ctccacggat gctcctgatc gcgttcggca gcgtccgccc ggcgcgatca tgctttagcc
       61 agagcgtgat cacgctggcg gaacgtccga agcgtgtaaa tcgcgcgata aaacaaggag
      121 gtagactatt tgcggcctgc acagtcagga tgaaaatggt ctagccggag ggtgccgatg
      181 ctacaactcg tactgtttaa tgtaaccaac gggctgatca tcggtgcgtt ctacgtgctg
      241 atggcgcttg gcctgtcgct gattctcaac ctgtcaaacg ttatcaactt cgctcacggc
      301 aactttctgg cgctcggcgg ctacatcgcc tacgtgctga cgccctatgt cgggttctgg
      361 ggcgcgctga tcatatcccc tgtgctgacc gcggcgttcg gcttcctgat cgagcgtctg
      421 atgatacgcc gcgtctatac tcgcgatccc gtgtacagct tgctgctgac cttcggcctg
      481 gcctttatcg tccaggacgt atgccgctat atatggggcc cgaacggcct gccaatgagc
      541 ataccctcgg tcttgtcgtc gcctctgaca ccagacctgt tcttcatcac ctggtatcgc
      601 gtcttcatgg tggcgatcgt cattatcgcc gtcgccggcc tgttcgcctt cctgcgcttc
      661 acccgcgtcg gcatccgcat ccgcgccggc acgctggacc tcgatacagt agcggccctc
      721 ggcgtcaacg tcggcatgct gcgatccttg aatttcgccg ttggttcgtt gctggctggg
      781 cttgccggcg tcctggcggc cggccagatc ggccttaatc cgaacatggg cgacgacttg
      841 ctgatgccca gtttcatcgc catcatcgtc ggcggggtcg gcagcctgac gggcacgttg
      901 ttgggcggcc tgctgatcgg ggtcgccgga gcattgacga ccgtgttctg gccggcggcc
      961 agcgaggcca tcatttacgt catcatgctg gtcgttctgg tggcgcgtcc gcgcgggctc
     1021 ctgggcgaag aggggatgat ggcatgagcg tgacaaccgt cgccgaggca cccaaacccg
     1081 ccgcctcggt gcgtccctgg ctcaccgccg ccattgtctg gagcctgctc ataaccgtgc
     1141 cgctttggct accgatgctc ggcggctaca cggccctggc cggacgggtg atcgtgctcg
     1201 gcctcgccgc gatgtcattc aacctgttgc tgggcttcac cggcgtcatg agcttcggcc
     1261 acgccgccta ctttggcctc ggtgcttacg gagccggcct gacgctgaag ttcatcgccg
     1321 ccagcacccc tttggcgctg ttggcgggca tcctgcttgg cggcctcgcc ggcacattgt
     1381 tcggcctgct gatcgttcgc cgccgcggcg tttacttcgc catggtcacc gtcgcctttg
     1441 ggcagatcgc attctacatc gcttatagct ggaacgacct gactggcgga tacgacggct
     1501 tgcgtgggtt cagccgggcg ccgctgaacc tcggcatcac cacgatcgat atcaccaaca
     1561 atggcaccgc cttctattac ttcctgctgg tggtcttcgc gatcgccacc ggactgcagg
     1621 gcctgctgct cgcctcaccc ttcggccgga cgcgtctggc gatccgcgaa aacgagcgcc
     1681 gcgcgcgctt cctgggcatc ccaatcgagc ggcatatctg gctgtcgttc tcgatctcgt
     1741 gctgctttac cgcactggcc ggcgcgctat acgctttgct gaacaacttt gccgacccgt
     1801 tgggactgca ttattcgctg tcgggagaga tcgtgatcat gaccgtcatg ggcggcatgc
     1861 gtgccttctg gggcccgctg gtcggggccg cactgttcgt cgtgctgcaa gactatatct
     1921 cttcgatgac ggtcaactgg atgtccttcg tcggcctgat cttcgtcctg gtcgtcttgt
     1981 tctttccccg gggccttctg ggaatgctgc aaggaagcag ccggaaatga acatgctgga
     2041 tgttcgcgac gtaacgaaga agttcggcaa cctggttgcg gtcagcgggg tctcgatgca
     2101 ggtcgccccg ggcgaactgc gcgccatcat cggtcccaac ggggccggca aaaccacatt
     2161 cttcaacatg atctccggct ttttcgcccc caccgcaggc gacatcgtct ttgacggaac
     2221 cgccgtcacc aagctggcgg tgaccgaacg ggtcgccatg ggcatggcgc ggacgttcca
     2281 gataaccgag atttttccgg aattgaccgt ccgcgaaaac atccgcatcg gtgttgaaag
     2341 tgcccagggg ttccgcctgc gcttctggct cggggccagg cataaagccg atgtggacca
     2401 gggtatcgag gaagccctta cgcagaccgg gctgaccggc aaggccgacc ggcttgttgg
     2461 cgaactgtct catggcgatc aacgcgcggc ggaaatcgcc acagcattga cgctgaaacc
     2521 gcgcctgctg ctgctcgatg agcctaccgc cggcatgggc gagcacgaaa cctatgccgt
     2581 cgccaacctg atccgccgcc tgcacaggaa cagcgcctat acgatcgtgc tgatcgagca
     2641 cgacatgcgc gtcgtcttca atctggccga caagatcacc gtcctgggag agggcaagat
     2701 gctggcgg
//
LOCUS       DASWQN010000097         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_803133, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000097 DASWQN010000000
VERSION     DASWQN010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803133"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..906
                     /gene="flgG"
                     /locus_tag="VGC82_01570"
     CDS             121..906
                     /gene="flgG"
                     /locus_tag="VGC82_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEY0421994.1"
                     /translation="MGSLDIAGTGMRAQQTNVEVISNNMANMTTTGFKRRRAEFQDLI
                     YQNLRRVGANSSDSGTVVPSGAQVGLGVKTAAIYRINEQGNLQQTSNTLDLAIQGNGY
                     FQVTAPGGETAYTRDGTFALAPDGTLVSADGYTIQPGVQIPPAATGVTINTSGQVQVT
                     LAGQTAPQTVGQIQLAAFPNEAGLDAQGGNLFIQTAASGAPVTSNPGTAGFGSVMQGF
                     VETSNVNVVSEITNLITAQRAYEMNSKVITSSDEMMSTLTNLR"
     gene            911..1876
                     /gene="flgA"
                     /locus_tag="VGC82_01575"
     CDS             911..1876
                     /gene="flgA"
                     /locus_tag="VGC82_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEY0421995.1"
                     /translation="MRILACFLAALVCGQAQAAVLRGMTTLHGPDVCLKDLFDDAGNN
                     ADRVLGPGPEPGGRIIVEAAQLNAIARQYSVKWRSVSSADRAVLEWPGRPLRKEEVLD
                     AVRVAIAAAGAGGDVEIDVSGFTTLLVPAEATPAVTVAQLDHDSSTGHFTAALTVMAE
                     GMNPINTRVSGRAEEMMEAPVAVTRLLPDTVLRLNDVRVARVRTASLQNDVARSMDQI
                     VGMQLRRPVAAGQPLRLADLSRPPLVQRGTTVQIELTSAGLSVTGQAVAMDAGADGEK
                     IRVQNITSKAFLYARVVGPGQVRVTPDAQAAQLTAPARFDSRNDP"
     gene            1873..>2448
                     /locus_tag="VGC82_01580"
     CDS             1873..>2448
                     /locus_tag="VGC82_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEY0421996.1"
                     /translation="MRTWTLVRLGVIAMSLAGCGTLDRLSTIGRDPAMTGTEDPTKDP
                     KWRPVSMPMPAAHLAPSEANTLWRSGSRAFFKDQRAAQVGDIVTVLVRMNDNANLKNV
                     TSAARSSTESGGIPNFFGLEAILPKAVNPATLISASSSNNNTGTGQIQRNEAVTLRLA
                     GIVTQVLPNGNLVVAASQEFMVNSELRDLRVT"
BASE COUNT          470 a          776 c          794 g          408 t
ORIGIN      
        1 gttgggcacg ctgaaaatgg tctagcgatc attcaccgta ctatgcctgc atgatcggcg
       61 cgctttgggc gctccattgg catgatcgat ccgtgacaaa atgccacagg agaccaacca
      121 atgggttcgc tcgatatcgc cggcaccggg atgcgagccc agcagaccaa cgtcgaagtc
      181 atttccaaca acatggcaaa catgaccacg acgggcttca agcgccggcg ggcggagttc
      241 caggacctga tctaccagaa cttgcgccgg gtcggcgcca acagttccga tagcggcacc
      301 gtggtgccgt ccggcgcgca ggtcggcctc ggggtgaaga ccgcggcgat ctaccggatc
      361 aatgaacagg gcaacttgca gcaaacatcg aacacgctgg atctcgcgat ccagggtaac
      421 ggatacttcc aggtcaccgc gcccggtggc gaaaccgcct atacccgtga cggcacgttc
      481 gccctggcgc ctgacggcac tcttgtatct gcggatggct atacgatcca gccgggcgtt
      541 cagatcccgc cggccgcgac aggcgtgacg atcaacacgt cgggccaggt gcaagtgacg
      601 ctcgcgggcc agaccgcgcc gcagaccgtc ggccagattc aactggcggc gttccccaac
      661 gaagccggcc tggatgcgca gggcggcaac cttttcatcc agacagcggc ttccggcgca
      721 cccgtgacga gcaaccccgg cacggccggt ttcgggtccg tcatgcaagg gttcgtcgag
      781 acctccaacg tcaacgtggt cagcgagatc accaacctca tcaccgctca gcgcgcctac
      841 gaaatgaaca gcaaggtcat cacctccagc gacgagatga tgtccacact gaccaacctg
      901 cggtaacgcg atgcgcattt tggcttgttt cctggcggcg ctggtttgcg gccaggcgca
      961 ggcggccgtg ttgcgcggca tgacgacgct gcatgggcca gatgtctgcc tgaaagactt
     1021 gttcgacgac gcgggcaaca acgccgaccg cgtacttgga ccaggtcccg aacctggcgg
     1081 ccggatcatc gtcgaagcgg cgcagttgaa cgcgatcgcc cgccagtaca gtgtcaaatg
     1141 gcgatcggtc tccagcgccg accgcgccgt gctggaatgg ccgggccggc ctttgcgcaa
     1201 ggaggaagtc ctggacgccg tccgtgtcgc catcgcggca gccggtgccg gcggcgatgt
     1261 ggaaatcgat gtgtccggct ttaccacctt gctggtgccc gcggaggcaa caccggcggt
     1321 taccgtcgcg cagctcgacc acgacagcag caccgggcat tttaccgcgg cattgacggt
     1381 catggccgag ggaatgaacc cgatcaacac cagggtcagc ggacgggccg aggaaatgat
     1441 ggaggctccg gtggcggtaa cgcgactgtt gccggacacc gtgcttcggc tgaacgatgt
     1501 tcgggttgct cgcgtgcgga cggcttcgct gcagaacgac gttgcccggt cgatggatca
     1561 gatcgtcggg atgcaactcc gccggccggt ggcagcggga cagcccctgc gcctggcgga
     1621 tctgtcgcgt ccacccttgg tgcagcgtgg gacaacggtg cagatcgagc tgacgtcggc
     1681 gggcctgtcg gtcaccggcc aggccgtggc gatggatgcc ggggccgatg gcgagaagat
     1741 ccgggtgcag aacatcacat ccaaggcttt cctctatgct cgggtcgtcg gtccagggca
     1801 ggttcgggtg actcccgacg cccaagcggc ccagttgacc gccccggccc gcttcgacag
     1861 ccggaacgac ccatgagaac ctggacgctt gtgcggctgg gcgtcatcgc gatgtcgctg
     1921 gccgggtgcg gcacgctgga ccgtctttcg acaatcggtc gcgatccggc gatgaccgga
     1981 accgaggacc cgacgaaaga ccccaaatgg cggccggttt cgatgcccat gccggcggcc
     2041 catctcgcac cgagcgaggc gaacacgcta tggcgttcgg gttcccgagc tttcttcaag
     2101 gaccagcgcg ctgcgcaggt gggggatatc gtgacggtcc tggtcaggat gaatgacaac
     2161 gccaacctga agaacgtgac gtccgccgcc cgcagcagca ccgaatcggg tgggattccc
     2221 aactttttcg gtctagaggc aatcctgccg aaagcggtca atccggctac gctgatttcg
     2281 gcaagcagct cgaacaacaa taccggcacg gggcagatcc agcgcaatga agcggtcacg
     2341 ctgcgcctgg ccggcatcgt gacccaggtc ttgcccaacg gcaatctggt cgttgccgcc
     2401 tcgcaggagt tcatggtcaa cagcgagctg cgggacttgc gggtgacc
//
LOCUS       DASWQN010000098         3007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_11268, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000098 DASWQN010000000
VERSION     DASWQN010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11268"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VGC82_01585"
     CDS             complement(<1..517)
                     /locus_tag="VGC82_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0421997.1"
                     /translation="MTTVTGILPRASAVVVAIALALSHSPAASAEMTMRIGHFPNITH
                     IQALVAHGFSRQGKGWFEQRLGADVKIEWYIYNAGPSAMEAMFANSIDVTYVGPSPAI
                     NAYAKSGGREVRIIAGSVNGGSALVIQGDSTLKTPGDFRGKKIATPQFGNTQDVAARA
                     WLIAGGLKITQT"
     gene            complement(972..2141)
                     /locus_tag="VGC82_01590"
     CDS             complement(972..2141)
                     /locus_tag="VGC82_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0421998.1"
                     /translation="MRLGLALLIGSIGSVGMWSVVVVLPVVQAEFAATRGAVSLAFTL
                     TMLGFGLGGVLTGRITDRFGIVTAMALSIATLGSAYILAGLSSTLWQFILVHCLIGLG
                     TSATFGPLMAEASHWFERYRGLAVTIVASGNYVGGTIWPPLVNRGIQVADWRTTHIGI
                     GIFCAVAMTLVLLLLRAQIGGAKPRDHDNAPPPRIDLRLSTNMLTALLSVASIACCVA
                     MAMPQVHIVAYCGDLGYGVARGAEMLSLMMACGIVSRIGSGFLADRIGGIRTLLIGSA
                     AQGFALLFYLFFDSLTSLYIISAMFGLFQGGIVPSYAIIVRESMPAREAATRVGIVIM
                     ASVVGMSFGGWVSGVIFDATGSYAAAFANGLGWNALNITIMVALLLRARQRLALA"
     gene            2607..>3007
                     /locus_tag="VGC82_01595"
     CDS             2607..>3007
                     /locus_tag="VGC82_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEY0421999.1"
                     /translation="MVDVTAGQGAGSSGTGFPSLGEIGTILKRGDLALAFGILTILVV
                     LILPLPSLVLDLFLAISITLSILILMTSLFIQAPLEFSSFPTILLISTMLRLSLNLAS
                     TRLILARGHEGTAAAGHVIEAFGNFVMGGNFV"
BASE COUNT          578 a          975 c          945 g          509 t
ORIGIN      
        1 ccgtttgcgt gatcttcagc ccgccggcga tcagccaggc gcgcgcagcg acgtcctggg
       61 tattgccgaa ctgcggagtt gcgatcttct tgccacgaaa gtcgccagga gttttcaacg
      121 tggaatcgcc ctggatgacc agcgccgagc ccccgttgac ggacccggcg atgatgcgaa
      181 cctcccgccc gccggatttg gcataggcat tgatcgccgg actgggaccg acataggtta
      241 catcgatgga gttcgcgaac atcgcctcca tcgcgctcgg accggcattg tagatgtacc
      301 attcgatctt gacgtccgct ccgagccttt gctcgaacca gcccttgcct tggcgagaaa
      361 agccgtgggc gaccaaggct tgaatatgcg tgatattcgg aaaatggccg atccgcattg
      421 tcatctccgc gctcgcggca ggcgaatgcg acaacgcgag agcgattgcg acgaccacgg
      481 cggaagcgcg cggcagaata ccggtcaccg tcgtcatgag gaatttctcc gaaggaccgc
      541 caagtctttg ccgtcagctg ggcggcagcg ccatgtgcat gatgcagtca tgcgtatgca
      601 aggctcttcg gaagataaaa tcaaaagtca catgtttggt ttcggaacgg aaaggaaata
      661 gtccggaatg gcggcgcggc agcatttttg ccgcaccctt cctgtccacg acgccggcct
      721 ccctcagtta ccatcggtcg atgcaggtcg atgcaggtcc cgcgctggcg tgacgccgac
      781 gcgccacccc ggtttcgacg tggcgtaatc tgtccgtcag cgctcgaaca aagagcgacg
      841 ggcgcgtcct cttcgcgccg gacagccgat gcggctgctg ttcatgttgg cagtcgactc
      901 cggcgcgggc caacagtcgt gccttgacct tggtcgggat catgcgccgg acgtccgcgc
      961 gccgccctcg cctacgccag tgccagccgc tgccgcgccc ggagcaggag cgccaccatg
     1021 atggtgatgt tgagcgcatt ccaccccagc ccgttggcga acgccgcggc gtaggagccg
     1081 gtcgcatcga agatcacgcc ggacacccag ccgccgaacg acatgccgac caccgaggcc
     1141 atgatgacga tgccgacgcg ggtcgcggcc tcgcgcgccg gcatcgattc ccggacgatg
     1201 atggcgtagc tcggcacgat gccgccctga aacaggccga acatcgcgga gatgatgtag
     1261 agcgaggtca ggctgtcgaa aaacaggtag aacaacagcg caaagccctg cgccgccgac
     1321 ccgatcagaa gggtgcggat gcccccgatc cggtccgcca gaaatcccga accgatccgg
     1381 cttacgattc cgcacgccat catcaaggag agcatttcgg cgccgcgcgc gacgccgtag
     1441 ccaaggtcgc cgcaataggc gacgatatgg acctgcggca tggccatcgc cacacagcag
     1501 gcgatgctgg cgacgctcaa cagcgcggtc agcatattgg tcgagagccg caagtcgatc
     1561 cgcggtggcg gcgcattgtc gtgatcgcgc ggcttggcgc cgccgatctg ggctcgcagc
     1621 agcagcagca cgagcgtcat tgccaccgcg cagaaaatgc cgatgccgat gtgcgtggtg
     1681 cgccagtcgg caacctggat tccccggttc accagcggcg gccaaatcgt gccgccaaca
     1741 taattgccgc tggcgacgat ggttaccgcc agcccgcgat agcgttcgaa ccagtgcgag
     1801 gcctccgcca tcagcggccc gaacgtcgcc gaggtgccga gcccgatcag gcaatgcacc
     1861 aggatgaact gccacagcgt cgacgaaaga ccggcgagga tataggccga tcccagggtc
     1921 gcgatgctga gcgccatcgc cgtcacgatt ccgaagcgat cggtgatcct gccggtcaga
     1981 acgccgccaa ggccgaagcc gagcatggtc agcgtaaaag ccagcgatac cgcgccgcgg
     2041 gtggctgcga attcggcctg cacgaccggc agcaccacca ccaccgacca catgccgacg
     2101 ctgccgatcg agccgatcag gagcgcaagt ccaagcctca cccacgcctg acgcgagtca
     2161 ggggtgaaga cagccgattc gcgtggctcg gaaggggcgt gcacaggcgg caacttcgtc
     2221 gcgcccagcc ccgaggtcaa gcgacattgg cgcgataata ggcatgcaga ggcggcgtga
     2281 cggcccgggt cgcatgaagc ctcgctgctg ctccgtccct cggggcgata cgccagtatc
     2341 gaacccggaa tgacgaggtt ccccgatgcg cgattgcgca tctgaggttc gccgctgacg
     2401 cgacctcccg gaacgacggc cgttaacgct ttgctaacca tataccgggc aaaaattgcc
     2461 cagtggagtc gagtgtcgtc gaacgcgtaa gacggggagt acccgtggac gccagtgcgg
     2521 tgcctcacct tcgaaacggt ggtccttcgg ccgccgcgca gacgtttggc gcccgcgacc
     2581 ggcatacgcg gggggaagca gcaaccatgg tggacgtgac ggcaggtcag ggggctggga
     2641 gctcagggac cgggtttcca agcctcggcg aaatcggcac catcctcaag cgcggcgatc
     2701 tcgcgctggc cttcggcatc ctcaccatcc tggtggtgct gatcctgccc ctgccctcac
     2761 tggtgctcga tctttttctg gcgatctcga tcacgctgtc gatcctgatt ttgatgacct
     2821 cgctgttcat ccaggcgccg ttggaattct cgtcgtttcc gaccatcctg ctgatctcga
     2881 cgatgttgag gctgtcgctc aacctggcct cgacccggct gatcctggcg cgcggccatg
     2941 agggaaccgc cgccgcaggc cacgtcatcg aggcgttcgg caatttcgtc atgggcggca
     3001 atttcgt
//
LOCUS       DASWQN010000099         5173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_407067, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000099 DASWQN010000000
VERSION     DASWQN010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407067"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1179)
                     /locus_tag="VGC82_01600"
     CDS             complement(256..1179)
                     /locus_tag="VGC82_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY0422000.1"
                     /translation="MARHPSTITRRGIISAAAAAAAIVPSTVRSAPVWTKAAFEHAMA
                     ESGRPVSLSDAQFDAIQKRKPIAMKRIEAYLKDHLGAADPAVLAAFEAVPREYYHYDY
                     PDHRATPGDAYEDSPKPWALGYGSALSDYLGQAYMTQVCKPKPGEVTLEVGTGSGFQS
                     SLLSRIVKRSYSIEIIEPLGKAVGRIFAPIGYDNVRTRVGDGYYGWPEVEGGFDIIIV
                     TCAATFAPPDLFKQLKPGGRMIIPIGQPFKRGQVLYVYTKDAAGKIHSHRDIGVFFIP
                     MTGAIAKNQPVKPETVSDHPADKPITDAVPE"
     gene            complement(1140..3581)
                     /locus_tag="VGC82_01605"
     CDS             complement(1140..3581)
                     /locus_tag="VGC82_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422001.1"
                     /translation="MNLIPLFLISLATVGYETALTRYFAVAKWSEYGYWVISIVMVGF
                     ALSGVILALWRDFFARRAAILMAALPAILVLTAAAGYRFTTTNPFNPLQLQNPATWQP
                     QIWNIAGYYAALLPFFFLAGLFISLSFVRYADRIGRVYAFDLTGAGAGAASVLAAMYA
                     VHAFNLVPLLLVPLALAAAFIAGPYRVKATLAAILALCAAEAVLLLDNRAEYNDFKAI
                     YAPLHTPDAKIVESVNSPRGNYALLDDFTERVDTDISNNAGMLGVEGPPRSFGLYRDG
                     NRIAALPKEQPIQSGYAAAALDALPFQLIPHARVMLAGASGGFRALEILRLGASQIRV
                     LEPEPQLLRIMRDGLGPVLPIGQRPNVHVAGNGPLADAGRGVYDVVDLSADFLDASDT
                     NTTAFTREAIVSYLHVLAPGGMVSIPVSIRDFPVYALRMLATVRAALLSVGITDPAAH
                     VVVYRSAWSVRILVARDGWDAERIAAARKFCDDRSFDLSWYPGMDVAQARSNLYNDLP
                     AVSFDKGEVEADGSDDAIADEAHAVLSGDPTPSREQFNLSPITLDRPFFYAVLRLDRI
                     GTILRRLEILPQPEVGALVNLAVLAQAIVIAALVLLVPLAAPGRIKDPRAGVLRSVIY
                     FPALALGFLFIEIYLIEKASFWLGDRTSGFALVLTGMLVFSGLGSLRTDHYAAHPRHG
                     IALVAAVVLVWCVAALVVLEPLILATLDWPFVARAGLLLLLIAPLSVALGLPFPLGLN
                     RAGTGGLLPWAWGLNGAFSVVATPLANLIAREAGFSRVLLCAAILYGIALAAFPVMRK
                     SSVWHDIPAPSPAAE"
     gene            complement(3578..4486)
                     /locus_tag="VGC82_01610"
     CDS             complement(3578..4486)
                     /locus_tag="VGC82_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0422002.1"
                     /translation="MRRPLLLVSSLLFGLLTAPSLARQGALPSVAQQGALPSEVTDHF
                     FLSTDGVRLHYLEAGPPAARTLVFVPGWTMPAWIWLPQIQAFSRRYHVVAFDPRGQGD
                     SAVPASGYEPVRRGRDLAELLSRLGSRPVVVIGWSLGVLDTLASIQVAGDTNLAGLVL
                     VDNSVGEEPPPAYTPEPRHTGRPPSHPEAMKAFVRQMFHRPRPTLYLDRLTQATLRTP
                     EHASRLLLAYPVPRSYWKQALYATKVPVLYVIRPYWVAQAGNLMRNRPNTETEVFSDA
                     GHALFVDEPARFNAVTESFLRRRIWP"
     gene            complement(4563..>5173)
                     /locus_tag="VGC82_01615"
     CDS             complement(4563..>5173)
                     /locus_tag="VGC82_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY0422003.1"
                     /translation="KAVEKAGYRIGEDITFALDPASTEFFKDSKYHLEGEGKVLDSDG
                     IVRYYADLCARYPIVSIEDGCSEDDWETWKLLTDTLGKTVQLVGDDLFVTNPERLRRG
                     IELGSANAILIKVNQIGTLSETLETVELAHRAGWNAVMSHRSGETEDSTIADLAVATN
                     CGQIKTGSLARSDRTAKYNQLIRIEQQLAGAARYAGSTILRR"
BASE COUNT          953 a         1550 c         1822 g          848 t
ORIGIN      
        1 aagcccggcc atgacacgat ggtcgatgac cacgaatggc tcgttctact ccgggacaca
       61 gccatgacgc ggtggtcgat gaccacgaat ggctcgttct actccgggac acagccatga
      121 cacgatggtc gatgaccacg aatggctcgt tctactccgg gacacggcca tgacgcggta
      181 gtcgatgacc acgaacggct ctttctactt gaggcacggc cgtgacgcta tcgtcgataa
      241 cgccaacggc ttgtactact ccgggacggc gtccgttatc ggtttgtcgg cgggatggtc
      301 ggacacagtc tcgggcttga ccggctggtt cttggcgatc gcgccggtca ttgggataaa
      361 gaatacgccg atgtcgcggt gcgagtggat ctttcccgca gcatccttgg tgtacacata
      421 cagcacctgg ccgcgcttga atggctggcc gatggggatg atcatccggc cgccgggctt
      481 gagctgcttg aacaggtccg gcggggcgaa tgtcgccgca caagtaacga tgatgatgtc
      541 gaagccgcct tcgacctcgg gccagccgta gtagccatcg ccgacgcggg tgcggacgtt
      601 gtcgtatccg atcggtgcga agatgcggcc gactgccttg cctagcggct cgatgatctc
      661 gatcgagtag gatcttttga cgatccgcga cagcaggctg ctttggaagc cgctgccggt
      721 gccgacctcc agcgtgactt cgccgggctt cggcttgcac acctgggtca tgtaggcctg
      781 ccccaggtaa tccgacagcg ccgatccgta gcctagcgcc caaggcttcg gactgtcctc
      841 gtaagcgtcg ccgggcgtcg cccggtggtc ggggtagtcg tagtggtagt attcgcgggg
      901 cacggcctca aacgcggcca gaacggcggg atcggcggcg cccagatggt ccttcagata
      961 agcctcgatc cgcttcatcg cgatcggctt acgcttctgg atcgcgtcga actgggcatc
     1021 gctcaggctg accggacgac cggattcggc catcgcgtgc tcgaacgccg ccttggtcca
     1081 gacgggcgcc gaacggacag tggacggaac gatggctgcc gcggcggcgg cggccgagat
     1141 tattccgcgg cgggtgatgg tgctgggatg tcgtgccaaa ccgaactctt cctcatgacg
     1201 ggaaatgcag ccaaagcgat gccataaagg atcgcggcgc acaaaagaac cctgctgaag
     1261 ccggcttcgc gggcgatcag gttcgccagc ggcgtcgcca caacggaaaa cgctccgttc
     1321 agcccccacg cccacggcag caacccgcct gtccccgctc ggttcaggcc cagcgggaac
     1381 ggcagcccga gggctacgga tagcggggcg atcaacagca gcagcaggcc ggcgcgggcg
     1441 acgaacggcc agtccagcgt ggcgaggatc aacggttcca ggaccaccag ggccgcgacg
     1501 caccatacca ggaccaccgc ggcgaccaag gcgatgccgt ggcgcgggtg tgcggcataa
     1561 tggtcggtgc gcaggcttcc gaggccggag aacaccagca tgcccgtgag cacgagcgcg
     1621 aagccgctgg tgcggtcgcc cagccagaag ctggctttct cgatcaggta gatctcgatg
     1681 aacagaaaac cgagtgccag ggccgggaag tagatcacgg aacgcaacac accggcgcgt
     1741 gggtccttga tgcggccagg cgcggcgagc gggaccagca gcaccagggc ggcgatgacg
     1801 attgcctggg ccaggacggc gaggttgacc agggcgccga cctctggctg cggcaggatc
     1861 tccagccgcc tgaggatggt tccgatgcgg tccagccgca gcaccgcgta gaagaacggc
     1921 cggtcgagcg tgatcgggga caggttgaac tgctcgcgcg atggcgtcgg atcgccggaa
     1981 agtacggcgt gcgcttcgtc ggcgatggcg tcgtccgaac cgtcggcttc aacctcccct
     2041 ttgtcgaagg atacggcggg gaggtcgttg tacaggttgg accgcgcctg ggcgacatcc
     2101 atgccgggat accaggacag gtcgaaggac cggtcgtcgc agaatttgcg tgcggcggcg
     2161 atgcgctcgg cgtcccagcc atcgcgggcg accaggatgc ggacgctcca ggcggagcga
     2221 tagacaacga catgggcggc cggatcggtg atgcctaccg agagcaaggc ggcgcggacg
     2281 gtagcgagca tgcgcaaggc gtaaaccggg aagtcgcgga tggagacagg gatcgacacc
     2341 atcccgccgg gcgccaggac gtgcaggtag gacacgatgg cttcgcgggt gaaagccgtt
     2401 gtgttggtgt cggacgcatc gaggaagtcg gcggacaagt caacgacgtc ataaaccccc
     2461 cgtccggcgt cggcgagcgg gccgttgccc gcgacgtgga cgttcggccg ctgaccgatc
     2521 ggcagcacgg gaccgagccc atcgcgcatg atgcggagca gttgcggttc gggctccagc
     2581 acgcggattt gggacgcgcc gaggcggagg atctcaagcg cgcggaagcc cccggacgcg
     2641 cccgccagca tgacccgagc gtgcgggatc aactggaacg ggagtgcgtc gagggcggct
     2701 gcggcgtagc ccgactggat cggctgctcc ttgggcagcg cggcgatgcg gttgccgtcg
     2761 cgatacaggc cgaagctgcg cggggggccc tcgacgccca acatgccggc gttgttggag
     2821 atgtcggtgt caacgcgttc agtgaagtcg tccagcagcg cgtaattgcc ccggggcgag
     2881 ttgacagact ccacgatctt cgcatccggt gtatgaagcg gggcgtagat cgccttgaag
     2941 tcattgtatt cggcccggtt gtccagcaag agcacggcct cggcggcgca tagtgccagg
     3001 atcgcggcga gggtcgcctt gacgcggtat gggccggcga tgaaggcagc ggcgagggcc
     3061 agcgggacca gcagcaacgg cacgaggttg aacgcgtgca cggcgtacat cgcggcgagg
     3121 acgctggcag cccctgcccc ggcgccggtc aggtcgaacg cataaacgcg gccgatgcga
     3181 tcggcgtagc ggacgaaact caggctgatg aacaggccgg caaggaagaa gaacggcagc
     3241 aacgcggcgt agtacccggc gatgttccag atctgcggct gccacgtcgc cgggttctgc
     3301 aattgcagcg ggttgaacgg attggttgtc gtgaagcggt aaccggcggc ggcggtcagc
     3361 accaggatcg ccggaagggc cgccatcagg atcgccgctc gacgcgcgaa gaagtcccgc
     3421 cacagcgcca ggatcacgcc gctcagggcg aacccgacca tgacgatgga gatgacccag
     3481 tagccatatt cggaccactt ggcgaccgcg aagtagcggg tcagcgcggt ctcgtagccg
     3541 acggtggcga gggagatgag aaacagcggg atcaggttca tggccagatg cggcgccgca
     3601 ggaagctctc ggtcacggcg ttgaagcgcg ccggctcatc gacaaacaac gcgtggccgg
     3661 catcgctgaa cacttccgtt tcggtgttcg ggcggtttcg cataaggttc ccggcctgcg
     3721 caacccagta tggccggatt acgtaaagta ccggcacttt cgtggcatag agcgcttgtt
     3781 tccagtagga tcgcggcacc gggtaagcca gcagcaggcg gctggcatgt tccggagttc
     3841 gcaacgtggc ctgcgtcagc cggtccaggt acagcgttgg ccgcggccgg tggaacatct
     3901 gccgcacgaa ggccttcatc gcctccgggt ggctgggggg gcgtccggtg tggcgcggct
     3961 ccggcgtata cgccggcggc ggttcctcgc cgaccgagtt gtccaccagc accaggccgg
     4021 ccagattggt gtctccggcg acctgaatgg aggcaagtgt gtccagcaca ccaagcgacc
     4081 agccgatgac gacgacgggg cggctgccca ggcggctgag cagttcggcg aggtcgcggc
     4141 cacggcgcac cggttcgtag ccggaggcgg gaacggcgga gtcgccttgg ccgcggggat
     4201 cgaaggcgac gacgtgatag cggcgggaga atgcctggat ctgcggcagc catatccagg
     4261 ccggcatggt ccagccgggc acgaaaacca gggtgcgggc ggctggtggt cctgcttcca
     4321 ggtaatgcaa tcggacgccg tcggtggaca ggaagaaatg gtccgtcact tcgcttggca
     4381 gcgcgccttg ctgcgcgacg cttggcagcg cgccttgccg ggcgagcgac ggcgcggtca
     4441 gcaggccgaa cagcagactc gagaccagta gaagcgggcg gcgcatcggt cccagaagac
     4501 ctgcgggggt gcgccggtca atataaatcc gattgcaacg cgttaatctt ctggcggccg
     4561 cttcaccggc gcaggatcgt cgatcctgcg tagcgggcgg cgccggcgag ttgctgctcg
     4621 atgcggatga gctggttata cttggcggta cggtcgctgc gagccaggct gccggtcttg
     4681 atctggccgc agttggtggc gaccgccagg tcggcaatgg tggagtcctc ggtctcgccg
     4741 gaccggtggc tcatgacggc attccagccg gcacgatgcg ccagttcgac cgtttccaat
     4801 gtttccgaca atgtgccgat ctggttgacc ttgatcagga tggcattcgc cgatcccagt
     4861 tcgatgccgc ggcgcagacg ctccggatta gtcacgaaca ggtcgtcacc aacaagttgg
     4921 acggttttgc caagcgtgtc ggtcagcagc ttccaggttt cccagtcgtc ctccgaacag
     4981 ccatcctcga tcgagacgat gggatagcgg gcgcaaagat cggcgtagta gcggacgatg
     5041 ccgtccgaat cgagaacctt gccttcgcct tcaaggtgat atttgctgtc cttgaaaaac
     5101 tcggtcgagg ccgggtcgag ggcgaaggtg atgtcctcgc cgatccggta gccggctttc
     5161 tcgacggcct ttg
//
LOCUS       DASWQN010000100         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1990631, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000100 DASWQN010000000
VERSION     DASWQN010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990631"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="VGC82_01620"
     CDS             complement(<1..1229)
                     /locus_tag="VGC82_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422004.1"
                     /translation="MAETERFLSPTWYRVAALKPRLRPHVTVRMHRYRGQLWYVIGDG
                     LRNRVHRVSPAAYVLVASMDGTNSLDSLWSEAAVTLGEQAPSQDQVIELVGQLHSNDL
                     VAGDVPPDARELFERQSQAERSKLLQWLLNPLALRFPLLDPNEFLTRTLPFVRPLLGR
                     LGAIAWLATVLPALILAARYWPELTDNIADRVLPAENLLLIAAVYPLIKLMHELGHGY
                     TTRAHGGAVHELGVMLLVLLPMPYVEVSAAAGFRGKWTRCLVGAAGILVELFIAAVAL
                     YVWLLVEPGMVRAVAFNVMLTASISSLLFNGNPLLRYDGYYILSDAVEVPNLAQRGRQ
                     YWSYLVRRYLFGTPQTRDFASTMGERIWFLLYTPLSSAYRISVTIGIALFLMHEYPTA
                     GALLAVWGVATGVVLPVA"
     gene            complement(1233..>1682)
                     /locus_tag="VGC82_01625"
     CDS             complement(1233..>1682)
                     /locus_tag="VGC82_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY0422005.1"
                     /translation="EEKLRRSRIVSPIDGQVVSGDLSQLLGSPVERGKTLFEIAPLDR
                     YRVVLRADEREMRYISAGLQGSLLLAGMPNDSERFTITRITPIAEAKDGRNEFRVEAS
                     LNEPPGPGLRPGLEGVAKIETGPHRVIWVWSHGLFDWLRVTAWKWLP"
BASE COUNT          343 a          555 c          504 g          280 t
ORIGIN      
        1 gccaccggca ggacgacgcc agtcgccacc ccccaaacgg ccagcaacgc gccggcggtg
       61 ggatattcgt gcatgaggaa cagcgcgatc ccgattgtca ctgaaatccg gtatgccgac
      121 gacagcggcg tatacagcag gaaccagatc cgttcaccca tggtcgaggc aaagtcgcgg
      181 gtctggggcg tcccaaacag atagcgacgc accaggtacg accagtattg ccgtccgcgc
      241 tgtgccaagt tcggcacttc aaccgcatcc gataggatat aatatccgtc gtagcgaagt
      301 agcggattgc cgttgaacag cagggacgag atactggctg tcagcatgac gttgaaagcg
      361 acagcccgga ccatcccggg ctccaccagc agccagacat aaagcgcgac cgcggcgatg
      421 aagagctcga ccaggatacc cgccgcaccg accaggcaac gtgtccattt gccgcggaaa
      481 cctgccgccg cgctcacctc gacatacggc atgggcaaca gcaccagcag catgacgcca
      541 agttcgtgta ccgcgccgcc atgcgcacgg gtggtgtagc cgtggccaag ttcgtgcatc
      601 aacttgatta agggatacac cgcggcgatc agcagcaggt tttccgccgg cagcacgcgg
      661 tcagcgatat tatcggtcaa ttcgggccag taccgtgcgg ccaggatcag cgccggcaac
      721 acggttgcca gccatgcaat cgcgccgagg cgccccagca gaggccgaac gaagggcagg
      781 gtgcgggtca ggaattcgtt cgggtccagc agcgggaagc gcaacgccag tggattcagc
      841 agccattgca gcaacttgct acgctcggcc tggctctgac gctcgaacaa ctcacgcgca
      901 tcgggcggga catcgcccgc caccaggtcg ttgctgtgca actgccccac caactcgatc
      961 acctggtcct ggcttggggc ctgctcacca agggtcacgg ccgcttccga ccacagtgag
     1021 tccaggctgt ttgtcccatc cattgatgcg actaggacgt aggcggcggg ggaaacgcga
     1081 tgcacgcggt tgcgcaggcc gtcgccgatg acataccaaa gctgcccacg atagcgatgc
     1141 atccgcaccg tcacatgcgg gcgcagccgc ggcttcagcg ccgcaacgcg ataccaggtt
     1201 ggactgagaa agcgttccgt ctctgccatg gttcagggca gccacttcca ggctgtcacg
     1261 cgcagccagt cgaacagccc gtgactccac acccagatca cccggtgggg gccggtttcg
     1321 atcttcgcca cgccctccag cccgggtcgt agtcccggtc ccggcggctc attcaggctc
     1381 gcttcgacgc gaaactcatt tcggccatcc ttggcctccg caatcggcgt gatccgggtg
     1441 atggtgaaac gctcgctgtc gtttggcatg cccgccagca acaggcttcc ttgaagtccg
     1501 gctgagatat agcgcatctc ccgttcatcc gcgcgtaaca ccacgcgata gcggtcgagt
     1561 ggcgcgatct cgaacaaggt cttgccgcgc tcgacgggcg accccagcag ttggctgaga
     1621 tcgccgctga cgacctggcc gtcgatcggc gatacgatcc gtgaccgtcg caacttttcc
     1681 tc
//
LOCUS       DASWQN010000101         2667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_671185, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000101 DASWQN010000000
VERSION     DASWQN010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671185"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VGC82_01630"
     CDS             <1..270
                     /locus_tag="VGC82_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422006.1"
                     /translation="AWTQAYCDNFSDRTPLVSRIALAATKLGNDPHNQEIAQCMLMDF
                     GTNRQPDRDKLLLAAAYHTAMHRKYGDAQEPSRRFGHAMGLAELH"
     gene            506..1399
                     /locus_tag="VGC82_01635"
     CDS             506..1399
                     /locus_tag="VGC82_01635"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0422007.1"
                     /translation="MTLSIKSIDPVSRPFFGGEVSGIDITRPLTADEAAGIEQGMDQF
                     GVLVFHGQRFDDESQLAFSRNFGPLEEASGDLNWGKPRRIESALVNDISNLDADNQVL
                     ARDNRRRLFSLGNRLWHSDSSFKEVPAKFSLLSARRIPSAGGNTEFADMRAAYDSLDQ
                     ATKAEIEDLVCEHSQLYSRALLGFADFTDEDRIRFKPVLQRLVRRHPSTGRKSLYLAS
                     HAGTIVGWPVPEARALLRDLIEHATQRQFVYAHEWKQWDLVMWDNRATMHRARPFDAK
                     EVRDMHRTTVGCERSTMAEAA"
     gene            1453..1905
                     /locus_tag="VGC82_01640"
     CDS             1453..1905
                     /locus_tag="VGC82_01640"
                     /inference="COORDINATES: protein motif:HMM:NF023125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HEY0422008.1"
                     /translation="MRASLLRAYRETTYETTCYETTGYGTARYETAGYETGDIVVRIG
                     RRVPAMDKLLSSAGAKQAVFITAYNPFSRVMPPGWNNRMQARLAEALRRYPLLPARGH
                     YRDWSERHLLALADVRVMRRLARRFRQNAIVIVRWRQPVRLLVTSSLM"
     gene            2039..>2667
                     /locus_tag="VGC82_01645"
     CDS             2039..>2667
                     /locus_tag="VGC82_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0422009.1"
                     /translation="MQNIDFSTRQRDVSEQTLDHPPRRRTSAAIYIHDLSPGGVERQS
                     LVLADELRARGVDIVLVLHQLRGELIHLLPPGIPVVNLRSARTLQDVFRLRRYLQTEQ
                     PDVLVANVDHNNIAAALANILAGTKTKLIICQHNPLSAGFHATVNWKHRLVPMFYKVL
                     SSRIDHAVAVSDGIARELINAGLMPAKVSTILNAVIGDDFHDRSREPVS"
BASE COUNT          517 a          821 c          810 g          519 t
ORIGIN      
        1 gcctggacgc aggcgtattg cgacaacttc agcgatcgca ccccgctggt cagccgcatt
       61 gcgttggcgg ccaccaagct tggcaacgat ccgcacaatc aggaaatcgc gcagtgcatg
      121 ctgatggatt tcggcaccaa ccggcagccc gatcgcgaca agctgttgct ggccgctgcc
      181 taccacaccg cgatgcaccg caaatacggc gatgcgcagg agccatcccg ccgcttcggc
      241 cacgccatgg gcctggcgga actgcactaa gggttagtcc gtcactaaat gctttgggtc
      301 gagcgcgcgc cggtggatcc gtcatggtgg tgggaccatg cactaatcgg agcggttgat
      361 catgactgga cctggaaccc cctgacagcc gtgcaagctg ggtgttcctg tcccgcaacg
      421 gtgtggcggg cgccaagcat gggcctgacg ccatgatcaa ccgctgccgg tgcttgatgc
      481 cgggttacga cgaggaaaca gcaccatgac gctctcgatc aaatccattg accctgtttc
      541 ccgcccattc ttcggcggtg aggtctcggg catcgatatc acccgcccgt tgaccgccga
      601 cgaggctgcc ggcatcgagc aaggcatgga ccagttcggc gtgctggtgt tccacggcca
      661 acgcttcgac gacgagtcgc agttggcgtt ctcccgcaac ttcggtccgt tggaggaagc
      721 cagtggggac ctgaactggg gcaagccgcg gcggatcgaa tcggcgctgg tgaacgacat
      781 ctccaacctc gatgccgaca accaggtgct ggcgcgcgac aaccgccggc gcctgttcag
      841 cctgggtaac cggttgtggc actcggacag ttccttcaaa gaggtcccgg ccaagttctc
      901 gctgctatcg gctcgtcgta tcccatcggc cggcggcaat accgagttcg ccgacatgcg
      961 cgccgcctac gattccctcg atcaggcgac gaaagccgag atcgaggatc tggtgtgcga
     1021 acacagccag ctttattctc gcgcgctgtt aggcttcgcc gatttcaccg acgaagaccg
     1081 catccgcttt aagccggtgc tgcaacgcct ggtgcgccgg catccgtcca ccgggcgcaa
     1141 atctctgtac ctggcctctc acgccggcac catcgtcggc tggccggtgc ccgaggcgcg
     1201 cgccttgctg cgcgacctga tcgagcacgc cacccagcgt caattcgttt atgcgcatga
     1261 atggaaacag tgggacctgg tgatgtggga caaccgggcg accatgcacc gtgcacgtcc
     1321 gttcgatgcg aaagaagtgc gcgacatgca ccgtacgacc gtcggctgcg aacgttcgac
     1381 gatggcagag gcggcctgag gccagccaaa ctccgcccgg tcctgcaacg ccggtgcggg
     1441 acaacgccgc cggtgcgggc ctcgcttctg agggcgtacc gcgagaccac atacgaaacg
     1501 acctgttacg agacgaccgg ctacgggacg gcccgctacg aaacggccgg ctacgaaacg
     1561 ggcgatatcg ttgtccgcat cgggcggcgc gttccggcga tggacaagtt gctgtcgtcc
     1621 gctggtgcaa aacaggctgt cttcatcacc gcctacaatc cgttttcccg ggtgatgccg
     1681 ccagggtgga ataatcggat gcaagcgcgg ttggccgagg cgttgcgacg gtatccgtta
     1741 ttgccggctc gggggcacta tcgagactgg tcggaacgcc acttgctggc gctggcggat
     1801 gttcgcgtga tgcggcggct ggcacgccga tttcggcaaa acgccattgt gatcgtccgc
     1861 tggaggcaac ccgttcgact tctggtaact tccagtctca tgtgatcacg tcggcttcgc
     1921 tggaacgctg aaggtccggc attgcggggc gcattgcctg gacttcggtc tcgcccatta
     1981 ttccatgccg tttgcgacaa attaaattca ccccggcttg cctcagcacg gagttactgt
     2041 gcagaacatc gatttcagta cccgccaaag ggacgtgtca gaacaaaccc tggaccaccc
     2101 accaagaaga cgcacttcgg cggcgatcta tattcacgac ctgtcgcctg gcggtgtcga
     2161 acggcagtcc cttgtgctgg cggacgaact gcgggcacga ggcgtcgata ttgtgctggt
     2221 tttgcaccag ttgcgcggcg aattgattca cctgttgccg cctggcattc cggtggtgaa
     2281 cctaagaagc gcgcgaacct tgcaggacgt ttttcgtttg cggcgttatc tacaaaccga
     2341 gcagcccgat gtgctggtgg cgaatgtaga tcacaataat atcgcggcgg cactggccaa
     2401 tatattggct gggacaaaga caaagttgat catctgccag cataatccgc taagcgccgg
     2461 ctttcacgcg acagtgaact ggaaacaccg tcttgttccc atgttctaca aagtgttgtc
     2521 atcccgcatc gatcacgcgg tcgcggtatc cgatggcatt gcgcgggaac tgatcaacgc
     2581 gggtctgatg ccggccaagg tcagcaccat tcttaacgcg gtgatcggcg acgatttcca
     2641 cgaccgatcg agggaaccgg tcagcca
//
LOCUS       DASWQN010000102         5562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1199240, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000102 DASWQN010000000
VERSION     DASWQN010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199240"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VGC82_01650"
     CDS             complement(<1..322)
                     /locus_tag="VGC82_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422010.1"
                     /translation="MMSIIRSALVAAFLAPACAAAAQENDLREFRVGMPVSALPATGY
                     TAFTCAGDPSKMFSTWGDYRSCPPAADGTRAVGFRYGDTGTLDSDKTQVGGQPVNLAL
                     LIDDQ"
     gene            complement(324..1031)
                     /locus_tag="VGC82_01655"
     CDS             complement(324..1031)
                     /locus_tag="VGC82_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0422011.1"
                     /translation="MTIRNPGHRTAISFAVAGLAWVLAVAGSTGLSAQSPVPNDGVRI
                     FKSANCVGCHKWSGEGGGSYGGAAANLRKTSLTMEQIEETIRCGRPMTGMPHFQADAY
                     ADGRCFGLRQSDLPDGKMPPEPDHPLRPADIQTVATYVVASIKGRGEPTFGECQAYFG
                     TGSRVCDIYAKQDGNSVRASSSPDSTPPSGSAMPSGQMSSGQTPPGQMPAGQMPAGHM
                     KVEAATDANAAGTSPGK"
     gene            complement(1129..2889)
                     /locus_tag="VGC82_01660"
     CDS             complement(1129..2889)
                     /locus_tag="VGC82_01660"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEY0422012.1"
                     /translation="MSKSTRVAAIVALVLSGQASAFAAGEVTADQLLKADSDSANWLT
                     HHRDYSGQRFSPLNQINKDTVKNLHVAWTLQLGGVEGGGIWSHGGLEGTPIVQNGFMY
                     VTDGWGTVYKIDTKGGQAKVLWKMDPKTDHDWAGAVACCGVDNRGVALSGNLVLSHSL
                     DGRLIATNKDTGEVVWQRQVADPDKSEVITAAPLVVKNLVITGVAGAEYGIRGWIAAT
                     DLVTQKEVWRTYTIPAKGDPGIETWKDGANAAATGGGSTWVTGTYDPSSNTLFWGVGN
                     PGPDWDSQYRPGDNLYTDSTLALDLDTGKIKWHFQHTPNDAFDYDSVSENVLVDAAVN
                     GRPMHLDLEADRNGFAYALNRDNGSFVWGVPFVKKLTWTKGLDQETGKPVEYDPKAQV
                     QKYNASVTPSRDNKETMICPGNMGGKNWPPTAYNPLLKQWYIPVIESCNRITVKPEVE
                     GQAVKPRDLFTGGGPTQPERITGSVTAIDVTTGKQVGKYETPFPMLGGLLATPDLVFY
                     GQPDGKVAALDAKSLQELWSFNTGGGVNAPPMTFTSDGKQYIAILVGQGGAWDKWFID
                     STPELKAIHPSSALYVFSLN"
     gene            complement(2935..3216)
                     /locus_tag="VGC82_01665"
     CDS             complement(2935..3216)
                     /locus_tag="VGC82_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422013.1"
                     /translation="MRKLSLSIVLSTALIFSLSLSNAVLADDAAESAKTRTSGSVKPG
                     LGSGPAEQKSAPSSAPPATTTRTTGATDQSNTVKTMNKNENAKLDVEGK"
     gene            complement(3241..5535)
                     /locus_tag="VGC82_01670"
     CDS             complement(3241..5535)
                     /locus_tag="VGC82_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0422014.1"
                     /translation="MNANFEPRIENVSRRTLLKGMGITAGLVLATPVLSRSGMAAYST
                     GAPNMPHGTVNDPRVFVSIAPDGIVTIIAARSEMGTGVRTSLPLIVAEEMEADWTRVH
                     VRQAPGNEEKYGNQDTDGSRSTRHYLIPMRQVGAAARMMLESAAAKRWNVAVTEVKAV
                     NHEVVHTGTGRKLGFGELAADAANQPVVEASKLRLKDPKDFRYIGKGQVGMVDLHDIT
                     TGKAMYGGDTRLPGMKYAAIARPPVTGGKLVSFDATDAMKVSGVEKVIEVQGWPWPSK
                     FQPLGGVAVIARNTGAAIMGRDALKVVWDDGPNAKYDSVSYRKELEEASRKPGLVVRD
                     AGNAERALQSADKVVVGEYYLPHIAHVSMEPPVATAHVANGKAEIWAPVQSPGGTRDD
                     VAKTLGIPAENVTINVPLLGGGFGRKSKCDFALEAALISRAINAPVKVQWTREDDIRH
                     DFIHTVSAERIEAGLDKSGKVVAWRHRSVAPTIGSTFAAGANHQKPFELAMGLVDLPF
                     DIANIRCENPEAAAHTRIGWFRSVSNIPHAFAVQTMVAEIAQQTGRDPKDVLLELIGP
                     ARIVDLSSSVKDPWNYGEPFDSYPIDAGRLRRVVELVAEKGEWGRSLPKGHGLGIAAH
                     RSFVSYVATIVEVAVDDKGKLTVPRVDTAIDCGTYVNPERIRSQIEGAAIMGLSLAKW
                     GELTFKDGRVVQGNFDDFPVVRIDESPQVTNVHIMEAGPDVPPSGVGEPGVPPFAPAL
                     CNAIFAATGKRIRSLPIGKQLEST"
BASE COUNT          986 a         1775 c         1633 g         1168 t
ORIGIN      
        1 cctggtcgtc gatcagcaaa gcaaggttga ctggctggcc gccgacctga gtcttgtcgc
       61 tgtccagggt gccggtatcg ccgtaacgga agccgactgc tcgtgtgccg tccgccgccg
      121 gtggacatga acggtagtca ccccaggtcg agaacatctt tgacggatcg ccagcgcagg
      181 tgaatgccgt atacccggtg gccggcagag ctgataccgg catgccgacg cggaattccc
      241 gcaggtcatt ctcctgcgcg gccgcggcac aggccggggc cagaaaagcg gcgacaaggg
      301 cgctgcggat gatactcatc atgtcacttt ccgggcgatg tgccggccgc attcgcgtcg
      361 gtagccgcct ccaccttcat atgtcccgct ggcatttgcc ccgctggcat ctgtccgggt
      421 ggcgtttgtc ccgaggacat ttgtcccgag ggcatggccg agcctgacgg tggggtggaa
      481 tctggcgaag atgaggcgcg aacactgttg ccatcctgct tggcgtagat atcgcagacc
      541 cgcgaacctg tcccgaaata cgcctggcat tcgccaaaag tcggctcgcc cctgcccttg
      601 atcgatgcca ccacgtatgt cgccacggtc tgtatgtcgg caggccgcag cgggtgatca
      661 ggttccggcg gcatcttccc atccggcaaa tcggattgcc tgagcccaaa gcagcgcccg
      721 tcggcgtagg cgtctgcctg gaaatgcggc atgcccgtca tcgggcgtcc gcaacgaatg
      781 gtttcctcga tctgctccat cgtcaggctg gtctttcgaa gatttgccgc ggcaccgccg
      841 taactgccgc cgccctcgcc cgaccatttg tgacagccca cacagttcgc cgacttgaaa
      901 atgcgaaccc cgtcattcgg gacgggggac tgagcgctta accctgtcga acctgcaacc
      961 gccagtaccc atgcgagccc ggcaactgcg aacgagattg cggttcggtg tccaggattc
     1021 ctgatcgtca tcgagatttt cctgacgcag tgggccggcg cgaccacttc cgggcaatcc
     1081 gaaatggtca cgacgccacc ggcaagtcca agccggcttc gaaagccgtt agttcagcga
     1141 aaagacataa agcgcggaac tcgggtgtat cgccttgagt tccggagtcg agtcgataaa
     1201 ccatttatcc caagccccac cctgacccac caggattgcg atgtattgct tgccatcgga
     1261 ggtgaatgtc atcggcggcg cgttgacgcc accacccgta ttgaagctcc aaagctcctg
     1321 caacgacttt gcatccaaag cagcgacctt accgtctggc tggccgtaga acactaggtc
     1381 cggagtcgcc agaaggccgc ccaacatcgg gaacggcgtc tcgtatttgc cgacctgctt
     1441 tccagtggtg acatcgattg cggtgacgct gccggtgatc cgctcgggct gggtcgggcc
     1501 accgccggtg aacagatccc gcggcttgac cgcctgacct tctacctcgg gtttgaccgt
     1561 gatgcggttg cagctttcga tcacggggat ataccattgc ttgagcagcg ggttatatgc
     1621 cgtcggcggc caattcttgc cgcccatgtt gcccgggcaa atcattgttt ctttgttgtc
     1681 gcggctaggt gtgaccgacg cattgtattt ctgcacttgc gcctttgggt cgtattcgac
     1741 cggtttgccg gtttcctgat ccagcccctt ggtccaggtc agtttcttga cgaacggcac
     1801 gccccacaca aacgaaccgt tgtcgcggtt aagcgcgtag gcgaagccat tgcggtcggc
     1861 ctccaggtcc agatgcatcg ggcgtccgtt taccgcggcg tccaccagca cattctccga
     1921 cacgctgtca tagtcgaagg cgtcgttcgg cgtgtgctgg aaatgccact tgatcttccc
     1981 ggtatcgaga tccaacgcga gcgtggagtc cgtgtacagg ttatccccgg gccgatactg
     2041 cgaatcccaa tccggtccgg gattgccgac accccagaac agcgtgttcg acgacggatc
     2101 gtaggttccg gttacccagg tcgagccgcc gccggtcgcg gctgcgttgg cgccatcctt
     2161 ccaggtctcg atgccgggat cacctttcgc gggtatggtg taggtgcgcc atacctcttt
     2221 ctgggtcacc aggtctgttg cggcaatcca gccgcgaatg ccgtactctg caccggcaac
     2281 gccggtgatg acgagattct tgaccacgag tggggcagcg gtgatgacct cacttttgtc
     2341 gggatcagcg acctgccgct gccagacaac ctcaccggtg tccttgttcg tcgcgatcag
     2401 gcgtccatcc aacgagtgcg agagcaccaa attgcccgac agggccacgc cgcggttgtc
     2461 cacgccacag caggcaacgg cgccggccca gtcgtgatcg gtctttggat ccatcttcca
     2521 taacaccttg gcctggccgc ccttggtgtc gatcttgtaa accgtgcccc aaccgtccgt
     2581 tacatacatg aacccgttct gaacgatcgg agtgccttcc aatccgccat gcgaccagat
     2641 gccaccgcct tcgacgccgc cgagttgcaa ggtccacgcg acatgcaggt tcttgacggt
     2701 gtccttgttg atctggttca gcggcgagaa gcgctggccg gaataatcgc gatgatgcgt
     2761 aagccagttc gccgaatcag aatcagcctt cagcagttgg tccgccgtca cctcgccggc
     2821 ggcgaaggca ctcgcctgac cggaaaggac aagggccacg attgccgcta ctcgcgtgct
     2881 cttggacatt tgcttccatc ccctcaaaga ttggcccctc agattcgtcc gacgctactt
     2941 tccttcgacg tctaatttcg cattttcatt tttattcatc gtcttgacag tgttgctctg
     3001 gtccgtcgcg ccggtcgtcc tggtggtggt cgcgggcggc gccgatgaag gcgccgattt
     3061 ctgctcagcc ggcccgctgc ccaaacccgg tttgacgctg ccggaggtcc tggtcttagc
     3121 cgattcggct gcatcatccg ccaggacagc attgctcagc gacagcgaaa agatcagcgc
     3181 tgtactgaga acgatcgata atgatagttt cctcatggat cgcgccccac tgttcaccgt
     3241 tcaggttgac tcgagttgct tgccgatcgg aaggctgcga atacgcttgc cggtcgccgc
     3301 aaagatcgca ttgcatagcg ccggcgcaaa cggcggcact cccggctcac ccactccgct
     3361 cggtggcaca tcaggacctg cctccatgat gtgaacgttc gtcacctgcg gcgactcgtc
     3421 aatccgaact acagggaagt catcgaagtt tccctgcacc acgcggccgt ccttgaatgt
     3481 cagctcgccc cacttcgcga ggctgaggcc catgatcgca gcaccctcga tctgcgaccg
     3541 gattcgttcc gggttgacgt aggtgccgca gtcgatcgcc gtatccacgc gcggaaccgt
     3601 cagcttgcct ttgtcgtcca ccgcgacttc gacgatcgtt gccacgtagc tgacgaaact
     3661 ccgatgggcg gcgatgccca atccatggcc cttcggcaga ctgcgccccc actcgccttt
     3721 ttcggcaacc aattcgacca cgcggcgcag tcggccggca tcgattggat agctgtcgaa
     3781 tggttcgccg taattccaag ggtccttgac cgatgagctg agatcgacga tgcgcgccgg
     3841 gccgatcaat tccagcaaaa cgtccttcgg atcacgcccg gtctgctggg cgatctcggc
     3901 gaccatcgtt tgaacagcga atgcgtgcgg gatattcgaa accgaccgaa accaaccaat
     3961 tctggtatgc gccgccgctt ccggattttc gcagcgaata ttggcaatgt cgaatggcag
     4021 atcgaccagt cccatagcga gttcgaaggg cttttggtga ttggcgccgg cggcaaaggt
     4081 ggagccaatc gtcggcgcga cgctgcgatg gcgccaggca acgactttcc cgcttttatc
     4141 cagaccggct tcaatccgtt cggccgatac cgtatggatg aagtcgtgcc gaatgtcgtc
     4201 ctcccgggtc cattgcactt tcaccggcgc gttgatggcg cgggaaatga gtgcggcttc
     4261 cagggcaaag tcgcacttgg acttccggcc aaaaccgccg ccgagcaatg gcacgttgat
     4321 ggtgacgttt tctgccggga tgccaagcgt cttggcgacg tcgtcgcgcg ttccgccagg
     4381 actttggaca ggtgcccaga tctccgcctt gccattggcg acatgcgccg ttgcgacggg
     4441 cggctccatg ctgacatggg cgatgtgggg caagtagtat tcgccgacga cgaccttgtc
     4501 ggcggactgc aaggcgcgtt cggcgttccc cgcatccctg accacgagcc ccggcttgcg
     4561 ggaagcctct tccagctcct tgcgatagct gaccgagtcg tatttcgcgt tcggcccgtc
     4621 gtcccacacc actttcaggg cgtcgcggcc catgatcgcg gcgccggtgt tgcgcgcgat
     4681 caccgcaacg ccgccaagcg gctgaaactt cgatggccag ggccagccct ggacctcgat
     4741 cactttctcc acacccgaca ccttcatggc atcggtggca tcgaatgaaa ccagttttcc
     4801 gccggtgaca ggtggccgcg cgatcgccgc gtacttcatg ccgggcagcc gcgtatcgcc
     4861 tccatacatg gccttgccgg tggtgatgtc gtgcaggtcg accatcccga cctggccctt
     4921 gccgatgtag cggaaatcct tcgggtcctt gagccgaagc ttggatgcct cgacgaccgg
     4981 ctggttggcg gcatcggccg ccagttcgcc aaagcctagt ttacgtccgg ttccggtatg
     5041 gacgacctcg tggtttaccg ccttgacttc agtaaccgcg acgttccaac gcttcgccgc
     5101 ggcggattcc agcatcatcc tggccgcggc acccacctgc cgcatcggga tcagatagtg
     5161 ccgtgtgctg cgtgatccgt cggtgtcctg gttgccgtat ttctcctcgt tgcccggcgc
     5221 ctggcggaca tgcacgcggg tccagtctgc ctccatttcc tccgcgacga tcagcggcag
     5281 gctggtgcgg acaccggttc ccatctcgga ccgagcggca atgatggtaa cgatgccgtc
     5341 gggtgcgatg gacacgaaga cccgcggatc gttgactgtg ccgtgcggca tgttgggggc
     5401 accggtcgaa taagctgcca taccactccg cgacagcacc ggcgtcgcaa ggacaagtcc
     5461 ggcggttatc cccatgccct tcagcagcgt gcgcctggaa acgttctcga tccgaggctc
     5521 gaagttcgcg ttcatggctc aggtccccgt agctgcgaga cg
//
LOCUS       DASWQN010000103         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_143805, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000103 DASWQN010000000
VERSION     DASWQN010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143805"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..>1574
                     /locus_tag="VGC82_01675"
     CDS             132..>1574
                     /locus_tag="VGC82_01675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0422015.1"
                     /translation="MTIAPPDRSSTKPDNSAFRQLLAVAALVVALTALRVIYASLIDL
                     RTDEAYYWTWSKENVLSFLDHPPMIAWFIRFGTAIFGDTNFGVRFAGILAMLVTQLLL
                     ADIVRRVTHDVRAVVLAVLMPEAALYYGLLMAKVSPDVALIPFAVAMVWALIRLNESG
                     DARWWLAAGVFAGLALLSKFTAVMLIPAVLAFMLVPDWRRRWLCSPYPWLAALMAVVL
                     FLPVLIWNAGHDWASFRFQFVRATATHEWSLRTVGDFIGLQFGLVGFILLPVVLSGLG
                     LTALRGYRRGDAVAILLSTAVIVPFAYFFWKSLTLRVGDTWPMFMWPIGFAAAAINVA
                     MLPREGRPAWMVKSTISWAVAAIASGIVMVVLIFLYYVVSPWNFIGRTDPIGGEAGYE
                     QVVDRAQAELQKIGATWIATTDYRTYAMLRWYFRDRVPVIQINERGRFLGFRDPGMSL
                     IRGHTGLYVGREPDNANPLWASTTAVREPLE"
BASE COUNT          247 a          445 c          514 g          368 t
ORIGIN      
        1 tatcgtttgg atgatggggt agaggccttc tgcaacaata aacgcccgtg gttctgggtc
       61 ccggcgttgg ccgggacgac gcgagagtgt gagtaagttg acacctccgg aggagtcggg
      121 gagaaagcat cgtgacgatt gccccgcccg atagaagcag caccaaaccg gacaattctg
      181 cctttcggca attgcttgcc gttgccgcct tggtcgttgc gctgacggcg ctgcgggtga
      241 tttacgcaag cctgatcgat ctgcgcaccg atgaggccta ttactggacc tggtcgaagg
      301 agaatgtgct ttccttcctc gatcatccgc caatgattgc ctggttcatt cgcttcggca
      361 ccgcgatctt cggcgacacc aatttcggcg taaggtttgc cggcatactt gcgatgctgg
      421 taacgcaact gctgcttgcc gatatcgtcc ggcgcgtgac gcatgacgtc cgcgcggtgg
      481 tgcttgcggt gctgatgccg gaggccgcgc tttattacgg cttgctgatg gccaaggtct
      541 cgcccgacgt tgccctgatt ccgtttgccg tcgcgatggt gtgggcgctg atccggctca
      601 acgaaagcgg cgatgcgcgg tggtggctgg ccgcgggcgt gttcgccggg ctggcgctgc
      661 tgtcgaaatt caccgcggtg atgctgatac cggcggtgct ggcattcatg ctggtgccgg
      721 actggcgccg gcgctggctg tgcagcccgt atccctggct cgcggcgctg atggcggtcg
      781 tgttgttcct gccggtcttg atctggaatg ccgggcatga ctgggcatcg ttcagattcc
      841 agttcgtacg tgcaaccgcg acccatgaat ggtcgttgcg caccgtcggc gatttcatcg
      901 gcctgcagtt tgggctggtc gggtttatcc tgctgccggt ggttttgtcg ggcctggggt
      961 tgacggcctt gcggggatat cgccgaggtg atgccgtggc cattctgctc tcgaccgctg
     1021 tgatcgttcc cttcgcttat tttttctgga aatcgctgac gctgcgggtc ggcgatacct
     1081 ggccgatgtt catgtggccc atcggatttg ccgccgccgc catcaacgtc gcaatgctgc
     1141 cgcgggaagg ccggccggca tggatggtca aatcgaccat ctcatgggcc gtcgcggcga
     1201 tcgcctccgg catcgtcatg gtggttctga tctttctcta ttatgtcgtc agcccctgga
     1261 atttcatcgg caggaccgat ccgatcggcg gcgaggcggg ttacgagcag gtcgtggatc
     1321 gggcgcaggc cgaattacaa aagatcggcg ccacctggat cgcaactacg gactaccgga
     1381 cctatgccat gctgcgctgg tattttagag atcgcgtgcc ggtaatccag atcaacgagc
     1441 gcgggcggtt tctgggcttt cgcgatcccg gcatgagctt gatcagaggt cacaccgggc
     1501 tctacgtcgg gcgtgagcca gacaatgcca atccattatg ggcttccacg acagcggtca
     1561 gagagccgct cgag
//
LOCUS       DASWQN010000104         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1331341, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000104 DASWQN010000000
VERSION     DASWQN010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331341"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1387
                     /locus_tag="VGC82_01680"
                     /pseudo
     CDS             281..1387
                     /locus_tag="VGC82_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
BASE COUNT          302 a          424 c          531 g          320 t
ORIGIN      
        1 aacatttctt gtaacgtatt gatatgtttg caaagtaagc ttctacgaaa aaggactata
       61 gactaaatcg tggataccga ttctctctta tataacaaca ggctgtcctg ctgtttagaa
      121 gatttctgca agaggctcag aggagattca gcgcttcgac gactaatact acagttgcgg
      181 ttttccgttt gcgattttcc gccaagacac catgtatcat tgcgagtgca gcgcagaagg
      241 ctgcgggttg aggcgtttga gggtattgat cgacgtgggc atggtggtga agccggtcgc
      301 tttgggcgag tggcgggatg aactagaaca ggtgcaggat cggctgggcg agctcttcgt
      361 gcggccggag ccgcggcagc aggcgggtct ttatctggaa gccctgctga gttcggccga
      421 acgcaagaat ggatggcagt tggccgaaca gatcggcgac gcccggccct ggcgcaccca
      481 acgggtattg tccgacgcgt tatggagcca ggacaaggcc cgcgccttgt gtcgcagcta
      541 tgtgatcgaa catttgggcg ctccggatgg tgttctggtc gtcgacgaga cgggcttcct
      601 gaagaaagga accaaatcgg tcggtgtcgc ccgccaatat tccggcacgg cggggcgggt
      661 tgaaaattgc cagatcgggg tgttcgtgag ctatggcagc agcaagggcc atgctctgat
      721 cgaccgggaa ctctacctgc ccaaggcctg gaccgacgat cgcgagcgct gtgacgcggt
      781 cagcgtgccg ccggagcggg cgttcatgac caagccacag ttggcgcagc tcatgatcga
      841 gcgcgccatc gctgcgggcg ttccgttctc ctgggtggtc ggcgacgagg tctacggcaa
      901 tgatcgccga ttgcggctgt ggctggagca gcaggagcgg ccgcatgtgc tcgcggtcag
      961 ggcgacggaa caggtgtggg cgcggattgc tgagcacgag cctgcgccaa tcccggtcaa
     1021 ggatctggcg acggcggagc cggcggacgg ctggcagcgg ctgagtgccg gggccggcag
     1081 caaaggcgag cggctgtacg actgggcgcg ctggcgcctg tttcgccagc aggagcagcc
     1141 gtgggaccat tggctgctgg tgcgccggaa gatcgccaat cccgaagagc tggcgtacta
     1201 cgtggtgttc ggtccggccg acaccagcct gcagaccttg gcgcaggttg ccggtcagag
     1261 gtggcgcatt gaagaatgtt tcgaagccgc caagcaggaa gtcggcctcg atgactacga
     1321 ggtccggagt tggcatggct ggtaccggca catcaccttg tcgatgctgg cgctggcctt
     1381 cctggcggtg atgtgcgtca aggccaatgc tgaaaaaggg gcgccagatc tgacggattt
     1441 cttccaattg ctttcagtat gccggagatc cgccgcctca tcgctcggtt ccaattgacc
     1501 gtcagcctct gcctcgatca catcctgcaa tggtccgtct ggcgccgcct ccatcaagcc
     1561 gtcgctaaat ggtgcca
//
LOCUS       DASWQN010000105         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1463583, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000105 DASWQN010000000
VERSION     DASWQN010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463583"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..821
                     /locus_tag="VGC82_01685"
     CDS             6..821
                     /locus_tag="VGC82_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0422016.1"
                     /translation="MWLVEADPNELEAAILNLAVNARDAMPDGGRLTIETSNAHLDEV
                     HVSAHSEVLPGQYIAISVTDTGTGMDARTIAQAFEPFFTTKPVGKGTGLGLSQVYGFV
                     KQSGGHVKICSKVGEGTTIKIYLPRLLGHPAGSEAFTDTPVPEGANEETILVLEDDDD
                     VRTYSVETLRELGYRVIEAHDGPSALRLLERQLRVDLLFTDVVLPGGITGAQVAAEAR
                     ALRPELRVLFTTGYARNAIVHHGRLDKGVQLITKPFSFPDLAAKVRDVLDEPS"
     gene            973..1542
                     /locus_tag="VGC82_01690"
     CDS             973..1542
                     /locus_tag="VGC82_01690"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0422017.1"
                     /translation="MHASPATADRILHVATEAVQSGENALLSALDQLSAAIYVTDPDG
                     LITYYNSACIEFAGRVPMVGRDRWCVTWKLYTEDGAFLPHDRCPMAVAILERRPIRGV
                     AAVAERPDGSRVNFMPYPTPIFDTEGYLLGAVNILVDVTDSRHADFLRSQARKCRRLA
                     ISVGDQQTADTLTLMAAEYEQKANEFCEV"
BASE COUNT          342 a          525 c          521 g          335 t
ORIGIN      
        1 acggtttgtg gctcgtcgag gcggacccca acgagctgga agccgcaatc ctcaatctcg
       61 cggtcaacgc gcgcgatgcg atgccggacg gtggccggtt aacgatcgag accagcaatg
      121 cgcacctcga tgaggtccac gtttctgcac attccgaagt gttgccgggt caatacatcg
      181 ccatctcggt caccgacacc ggaaccggaa tggacgccag gacgatcgcg caagcgttcg
      241 aacccttctt caccaccaag ccggtcggca agggcaccgg gctcggcctg agccaggtct
      301 atggcttcgt caagcagtcc ggcggccacg tgaaaatctg ttcgaaggtg ggcgaaggaa
      361 ccacgatcaa gatctatctt ccgcgactct tgggccatcc tgccggttct gaggctttca
      421 ccgatacgcc ggtgccggaa ggagcgaatg aggaaaccat cctcgtcctc gaagacgacg
      481 atgatgtgcg aacctattcg gtggagacgc tgcgcgaact gggttatcgg gtgatcgaag
      541 cccatgatgg cccgtctgcg cttcgcctgc tggaacggca actgcgcgtc gacctgttgt
      601 ttacggacgt ggtgctcccc ggcggcataa cgggcgcgca ggtcgctgcc gaggcgcgcg
      661 ccttgcgtcc ggaattaaga gttctgttca ccaccggcta tgcccgcaac gccattgtcc
      721 atcacggccg cctcgacaag ggagtgcaac tgatcaccaa gccgttcagc tttcccgatc
      781 tcgccgccaa ggtgcgggat gtgctggacg aaccatcgtg aatgattctg gcacagaccc
      841 ggcgtaatac tttgcaaggt gcggttcaat gctaccaatt cggatatata attgatcgga
      901 tggaactttc cggcgggttg cccgtcgggc attactgggt agacttggtc cgccggggct
      961 tggataaacg agatgcacgc atcgcccgca accgcggacc ggattctgca cgtggccacc
     1021 gaggcggtgc agagcggtga gaacgctcta ctatcggcgc tcgaccaact gtccgcggcc
     1081 atttacgtga ccgacccgga tggcctcatc acctactaca attcagcgtg cattgaattt
     1141 gccggcaggg tgccgatggt cggcagggat cgctggtgtg tgacctggaa gctttacaca
     1201 gaggacggcg ccttccttcc gcatgaccgc tgtcccatgg ccgtggcgat cctcgagagg
     1261 cggcccatca gaggcgtcgc ggcagtcgct gagcggcctg acggaagccg cgtgaacttc
     1321 atgccctacc ccacgccgat cttcgacacg gaaggctatc ttctcggtgc ggtgaacata
     1381 ctggtcgacg tcaccgacag ccggcatgcc gactttcttc gatctcaggc gcgcaaatgc
     1441 cgccgcctcg ccatctcggt tggcgaccag cagaccgccg acaccttgac gctgatggcc
     1501 gccgaatatg agcagaaggc caacgagttc tgcgaagtgt aaattccggc aatcgtcggg
     1561 ccttgctcct cgcattgccg gccggtgccg ggatgcggcg atcgcgcttg cggcacgatg
     1621 gggccacgct gatcaatccc agcaatttgc gttgactacg ataaggaccg gcccagaatg
     1681 gcgttccact ctcaacatag gaggcgagta tgtcatgcaa att
//
LOCUS       DASWQN010000106         3977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_540206, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000106 DASWQN010000000
VERSION     DASWQN010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540206"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1438)
                     /gene="eno"
                     /locus_tag="VGC82_01695"
     CDS             complement(92..1438)
                     /gene="eno"
                     /locus_tag="VGC82_01695"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY0422018.1"
                     /translation="MTDTTIAYVHGRRVWDSRGRPTVEADVLLEGGAVGRAIAPAGAS
                     TGSGEALDLRDGGSAFGGYDVTQAVGNVNTIIAHGVAGMDATDQEAVDERLIELDGTR
                     GKSRLGANALLSVSMAVAHAAASAAGQPLYRYLGDADTVLIPLPQIQIFGGGAHAGRR
                     VDIQDFMVLCPAADSFAQALDWTAEIYRAAGRLMEEAGGLAGVADEGGWWPSFSSNEQ
                     ALDTLVKAIEHAGFRPGDQVGIGLDVAASQFGRNGRYKLALDDKELTSEGMIEMLTGW
                     IDRYPILSIEDPLAEDDLAGFIAFSQAVGAKVQIVGDDFLVSDADRLRDAAAKGAGNA
                     VLLKPNQRGTLTETLDTRRAAADCGYATIASARSGETEDVTVAHLAVGWGTGQLKVGS
                     FTRGERMAKWNEMLRIEEALGGKAHFAGRSVLGRSGLSQSGPGQSVPGPLGPSART"
     gene            complement(1435..2436)
                     /locus_tag="VGC82_01700"
     CDS             complement(1435..2436)
                     /locus_tag="VGC82_01700"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY0422019.1"
                     /translation="MKMLPDNIIEALDRMHLLAGTVPTGEPLAGGVSSDIWRIDLPSG
                     PICFKRALAKLRVKADWFAPVERNIFEARWMRIAGQAVPGGTPELLGQDKVTGSLAMT
                     YLAPERYPLWKTVLRSGTVDTGFAASVASALARVHAATASNPLLAREFPTDAIFHDIR
                     LEPYLTHAGRAHPELAGRMQLLIETTIGNKHALVHGDVSPKNILCGPDGPVFLDAECA
                     WWGDPAFDLAFCLNHLMLKCLWIKEHQKALLESFEAMAATYLRLVTWELPETLEIRAA
                     HLLPALFLARVDGKSPVEYITQDVQRDQVRAVACRLLADPPDRLADVAQAWKEEVAA"
     gene            2532..3356
                     /locus_tag="VGC82_01705"
     CDS             2532..3356
                     /locus_tag="VGC82_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0422020.1"
                     /translation="MSRQRSGTMRQDRTSAPGLGTPHPPALLVRRRHVLPIAVSLSVA
                     AHIVAALLVVFLPRIFPPEDRLQEQGTVELLMVEQKGGEPRQGALQDAKPAAEPPPTG
                     AAPKQEDHEPGTPDHVLSEQGNEPVPPQVQQASPPRGEVDNQPTQRQMQAESVPARPP
                     EAPVFNLEGTESETNARALGDRILPAMPDNRFRNRPPVYPAEAEMKGDHGSVLLVIHV
                     AENGTAASVSISRSSGVKSLDEAAKAAIMKWRFHPAMHEGRTVPFDMPFQVIFEAN"
     gene            3366..3920
                     /locus_tag="VGC82_01710"
     CDS             3366..3920
                     /locus_tag="VGC82_01710"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY0422021.1"
                     /translation="MVRIDRVVTKGGDNGETSLGDGTRVRKTDPRIEAMGAVDEANAS
                     IGVLRIQTRSHPVEDAMLARIQNDLFDVGADLCVPGSAEDRLRLAGTAVSRLEAEVVR
                     MNEALPPLTSFILPGGTDAAAFAHVARVTARKAERAVLDVDDVNPVVQRYLNRLSDHL
                     FVLARVLNDSAVRDVLWIPGANRS"
BASE COUNT          776 a         1256 c         1212 g          733 t
ORIGIN      
        1 cctatttggg ggaaacaggg gacatttcta ctttgggttg acaggcgatt gatgcatctt
       61 ggtgcgcccc tctggtgatg agcacgatgc gtcaggtccg agcgctcggg cccaaaggac
      121 ccggcactga ttgacccggc cccgattggc tcaggcctga ccggcccagg accgaccggc
      181 cggcaaaatg ggctttcccg ccaagcgctt cctcgatacg caacatctca ttccacttcg
      241 ccatgcgttc gccgcgggtg aagctgccga ccttcaactg cccagtgccc cagccgacgg
      301 caagatgcgc gacggttacg tcctcggttt cacccgatcg agcagaggcg atcgtggcgt
      361 aaccacaatc cgcggccgct cgacgagtat ccaaagtctc ggtcaaggtg ccgcgctggt
      421 tgggtttcaa taaaacggcg ttgcccgcac ctttggcggc ggcatcgcga agccgatcgg
      481 cgtcgctgac cagaaaatca tcgccaacga tctggacctt tgcgccgaca gcctgtgaaa
      541 acgcaatgaa gcccgccaga tcgtcttccg ccaacgggtc ctcaatcgac aggatcggat
      601 agcggtcgat ccagccggtc agcatctcga tcatgccctc ggacgttagt tccttgtcgt
      661 ccagtgccag tttgtagcgt ccattcctgc cgaactgcga ggcggctaca tcaagcccaa
      721 tgccaacctg atcacctggg cggaagcctg cgtgttcaat ggccttcacc agtgtatcaa
      781 gcgcctgttc attgcttgaa aaggaaggcc accagccgcc ttcgtcggcc acgccggcga
      841 gaccaccggc ttcctccatc aaccggcccg cggcgcgata gatttctgcc gtccagtcca
      901 gcgcctgggc gaagctgtcg gcagccgggc acagcaccat gaaatcctgt atgtccaccc
      961 gccgcccggc atgagcaccg ccgccgaaaa tctggatctg cggcagtggg atcaggacgg
     1021 tgtcggcgtc ccccaggtag cggtacaaag gctgtccggc agccgatgcg gcggcatgtg
     1081 cgacggccat ggagaccgac aacagcgcat tcgcgccaag ccggctcttg ccgcgcgtcc
     1141 cgtccagttc gatcagccgc tcatccaccg cctcctgatc ggtcgcatcc ataccggcca
     1201 cgccgtgggc gatgatggta ttcacgttcc cgacggcttg cgtcacgtcg tagccgccga
     1261 atgccgaacc gccgtcgcgc agatccagcg cctcgcctga accggtggag gcgcccgcgg
     1321 gagcgatcgc gcgtccgacg gcaccgcctt ccagcaacac atcggcctcc accgttggcc
     1381 gtccccggct atcccaaacg cggcggccgt ggacataggc gatcgtcgtg tcggtcatgc
     1441 ggcgacttcc tctttccagg cttgggctac atcggccagg cgatcgggcg gatcagcgag
     1501 aagacgacat gcaacggcgc ggacctggtc gcgctgaacg tcctgggtga tatattccac
     1561 cggagatttc ccatccaccc gtgcaaggaa cagtgccggc aaaagatgcg ccgctcggat
     1621 ttctaatgtt tcaggcaatt cccatgtgac aagccgcaga tatgtggcag ccatcgcttc
     1681 gaagctttcc agcaatgcct tttggtgttc ctttatccat aagcacttca acatcagatg
     1741 gttcaggcag aatgccaggt cgaaggcggg atcgccccac caggcgcatt ccgcatcgag
     1801 gaataccgga ccgtccggac cacatagaat gttcttgggg ctgacatcac catgcaccaa
     1861 agcatgtttg ttgcctatcg tcgtttcgat caacaactgc atacgacctg ccaactcagg
     1921 gtgggcgcgt cctgcgtggg tcagataggg ctccagccgg atgtcatgaa aaatggcgtc
     1981 cgttggaaac tcccgggcca atagggggtt gctcgccgtc gcggcatgaa cccgcgccaa
     2041 cgccgatgcg acggacgccg caaaacctgt gtccacggtg ccgctccgca aaactgtctt
     2101 ccacaatggg taccgctccg gtgccaaata ggtcatcgcg agacttccgg tcaccttgtc
     2161 ctggcccaag agctccggcg tacctccggg gactgcctgt ccggcgatcc gcatccatcg
     2221 cgcttcaaag atgttgcgtt cgactggcgc aaaccagtcg gctttcacgc gtaacttggc
     2281 caaggcccgc ttgaagcaga tcggcccact gggaaggtcg atccgccaaa tgtcggaaga
     2341 tacgccgccg gccaagggct cgccagttgg cacggtaccg gcaagcaaat gcatccggtc
     2401 gagcgcttct atgatattgt cgggcagcat tttcatcgtg gccgtgatcc tacctcgctt
     2461 gcctcgtgcg gtcgaagtgc gaggatcgac acgcaggtcc tgtgtatgaa aagccgggag
     2521 atatggctac gttgagcagg cagcgctcgg gcacaatgag gcaggacagg acgtcggcac
     2581 cagggttggg gacccctcat ccgcccgcgc tgttggtccg gcgccggcac gtcctgccga
     2641 tcgcggtctc gctttcagtg gcggcccata tcgtagctgc cttgttggtc gtattcctgc
     2701 cgcgcatctt cccgccggag gatcgtttgc aggaacaagg caccgtggaa ttgctgatgg
     2761 tggaacaaaa aggcggagaa ccacgccagg gcgcgttgca ggatgccaaa cctgcggcag
     2821 aaccgccgcc aacgggcgcg gcaccaaagc aggaagacca cgagccgggc acgccggatc
     2881 atgttctatc cgagcaaggc aacgaaccgg ttcctccgca agtccagcag gcgtcgcctc
     2941 cgcgaggtga agtggacaac cagccgaccc aacggcagat gcaggcggaa tccgttcctg
     3001 cgcgtcctcc ggaagcaccg gtcttcaacc tggagggcac cgaaagcgaa accaacgcac
     3061 gcgcgctcgg tgaccgaatc ctgccggcca tgccggacaa caggttcagg aatcggccgc
     3121 cggtttatcc ggccgaagcg gagatgaagg gcgatcacgg ctcagtcctg ctcgtgatcc
     3181 atgtggcgga aaatggaacg gcggcaagtg tgagcatcag cagaagttca ggggtgaaat
     3241 cgctggatga ggccgcaaaa gcggccatca tgaaatggcg cttccacccc gccatgcacg
     3301 agggccggac cgtcccgttc gacatgccgt tccaggttat ctttgaggcg aattaggggg
     3361 ctagcatggt gcgtattgat cgcgttgtga ccaagggcgg cgacaatggg gagacctcgc
     3421 tcggcgatgg gacacgggtg cggaagaccg acccgcggat cgaggcgatg ggcgcggtcg
     3481 atgaggcaaa cgccagcatc ggagtccttc gcatccaaac caggagccat ccggtagagg
     3541 atgcgatgct ggcgcggatc cagaacgatc ttttcgatgt gggcgccgat ctgtgcgtgc
     3601 cgggatcggc ggaggaccgg cttcgccttg ccggcacggc cgtatcgcgc ctggaagccg
     3661 aggtcgtgcg catgaacgag gcgctcccgc ccctgacgag cttcatattg ccgggaggga
     3721 ccgatgcggc ggctttcgcc catgtggccc gcgtgaccgc ccgaaaagcg gagcgagccg
     3781 tgctggacgt cgatgacgtt aatccggtgg ttcaacgtta tctcaaccgg ctttcggacc
     3841 atcttttcgt tcttgcccgc gtgctcaacg atagtgccgt ccgcgatgtg ctttggatac
     3901 ccggcgccaa ccggtcgtga cggcatgcac ttgacagccg ccttagctct ggtttcgcat
     3961 ccgctcttct ggtctgg
//
LOCUS       DASWQN010000107         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1727630, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000107 DASWQN010000000
VERSION     DASWQN010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727630"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..773)
                     /locus_tag="VGC82_01715"
     CDS             complement(303..773)
                     /locus_tag="VGC82_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422022.1"
                     /translation="MNMIASRTLSQDYAPSGTSDIPTSSVVAIVSDNPATIENLAPVC
                     EFLDLKIELVSTAADLSHVLREHRPMAVISEVEGAGQDGFHTMKQVALYCRDLPILLL
                     TGGDNVLMGAADAIQDLWGLTAVASTSEFPIAGQLVAFLFNAGRRAGRLRLMSV"
     gene            complement(1060..>1559)
                     /locus_tag="VGC82_01720"
     CDS             complement(1060..>1559)
                     /locus_tag="VGC82_01720"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY0422023.1"
                     /translation="TGPSSEAVPEAKLPEKLPEPEKPEGAPARLPRFVSLRSDDVNMR
                     AGPGTRYPIEWVYKRRDLPVEIEREFDVWRWVRDPEGIQGWVHQATLIGRRNFIVQKA
                     DATLRSDPADSAAAVAILKPGVIGRIRACDAKSEWCSVQTGSYRGYLRRTQFWGLLPG
                     EAINP"
BASE COUNT          315 a          492 c          456 g          296 t
ORIGIN      
        1 gcaaagctac agcaacgaca cgctcgagcg gatgggatta cgaacgatcc gggcgaactg
       61 actcaagcgc attgggaagc ctatcatgct tcgatcgtgg agccgaacag tcggccaaac
      121 cggcccttcg gccgctatgc agcggatgct cgcaaacagc ggcaccaatg cacccgcgag
      181 ccggctacag cggattgatc gccgctgggc gtattccgtc cgcatagttc attcaatccg
      241 cgtgtcgggc gttctgccca gcgcgatcac gccctggtgc gacgaacttc ggaggcagga
      301 cactagaccg acatcaaccg caagcggccg gcacggcggc cagcattgaa caggaaggca
      361 acgagctggc ccgcgatcgg gaactcgctg gtgctggcga ccgccgtcag gccccacaaa
      421 tcctggatgg catcggccgc tcccatcaac acgttgtcgc cgccggtgag caaaaggatc
      481 ggcaggtcgc ggcaatacaa cgccacctgc ttcatcgtat gaaagccatc ctgaccggca
      541 ccttcgactt cgctgatcac cgccattgga cgatgttccc gcagaacatg cgaaagatcg
      601 gcggcggtcg aaacaagctc gattttaaga tcgagaaatt cgcatacggg agccaggttc
      661 tcgatcgtgg ccgggttgtc gcttacgatg gcgacaacgc ttgacgtcgg aatgtcggag
      721 gttcccgaag gggcgtaatc ctgagacagc gtccgactgg cgatcatatt catggcggtt
      781 ctgcttcctg caaacggagc tggcttctgc caggcgacag atcacagtct ggtgaaaata
      841 cccctgtagg tgtagcctcc gtggggataa ctctgcgttt gattacagta attctccgtt
      901 aattaccgtt aacgcgggga cggatccgcc aatcgtgaag gtgccgctta tgaggttgac
      961 ctgtaccgtg acggacgccg tggatatgct atttgtctga cgcgcagccg ccacaccaat
     1021 cccgcgcgga taccagccag ttcttggaac cacttggtat caggggttga ttgcttcgcc
     1081 cggcagcaag ccccagaact gcgtccgccg aagatatccc cgatacgatc cggtttgcac
     1141 gctgcaccat tcggatttgg cgtcgcaggc acgaatccgg ccgatcaccc ccggcttcaa
     1201 gatcgccact gccgccgccg agtcggcagg gtccgaccgc agcgtcgcgt ccgccttctg
     1261 cacgatgaag ttgcgtcgcc caatcagcgt tgcctggtgt acccaaccct ggatcccctc
     1321 ggggtcgcga acccaacgcc acacatcgaa ctcccgctcg atctccaccg gaaggtcgcg
     1381 gcgcttgtac acccattcta tcggatatcg tgtgcctggc ccggcgcgca tattgacgtc
     1441 gtcggagcga agcgagacga agcgtggcag cctggctggc gccccctccg gcttctcggg
     1501 ttccggcagt ttctccggta gcttggcttc aggaacagcc tcgctcgagg gacccgtcg
//
LOCUS       DASWQN010000108         5143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1727642, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000108 DASWQN010000000
VERSION     DASWQN010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727642"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            478..702
                     /locus_tag="VGC82_01725"
     CDS             478..702
                     /locus_tag="VGC82_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422024.1"
                     /translation="MPEVIFNHVNQFELLAALADAEWEMYELGSCLNIPGTLRLRLLQ
                     AAERARQLIDPEDEIGVYLIPDRGMTLRLP"
     gene            1150..1569
                     /locus_tag="VGC82_01730"
     CDS             1150..1569
                     /locus_tag="VGC82_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422025.1"
                     /translation="MTIGTNPPGGLAMAHRNSAHSKNFSTAPAPGRYHAVVWLDSRQA
                     RVTYVSRDDSEEGTVRRVDPPCQLHIEAARTSSKRAADAPAFYRDLAEACDKAKAVLL
                     MGPSTAKEDFLRYLHQHSPATFELVSRAAGDSPSATR"
     gene            1745..3070
                     /locus_tag="VGC82_01735"
     CDS             1745..3070
                     /locus_tag="VGC82_01735"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY0422026.1"
                     /translation="MRITMIGGGYVGLVSGACFAEFGTDVTVVETDPRKLSALRDGRM
                     PIYEPGLEQLVAENVRAGRLSFTSELAAAVDGTEAVFIAVGTPTRRGDGHADLTYVYA
                     AAEQVANALTGYAVIVTKSTVPVGTGRRIQEIVGATRPDLDFDVASNPEFLREGNAIG
                     DFMRPDRVVIGAESERSREIMRRLYRPLYLIEAPIVFTSIETAELTKYAANAFLAMKV
                     TFINEMADLCEKVGADVHDVARGIGLDGRIGRKFLHPGPGYGGSCFPKDTLALVRTAQ
                     DYGAPSRLVETVVAVNEARKSSMAMRVINACGGSVRGKTIAVLGLTFKPETDDMRDSP
                     SLPIVSRLAGDGATIRVFDPEGMEQARPLLPESVRYCADPFEAVAGADALVLITEWNE
                     FRAMSPSRLAETMRGRVMVDLRNVYDPVAMRQAGFEYHGIGRAVLPADG"
     gene            3063..4298
                     /locus_tag="VGC82_01740"
     CDS             3063..4298
                     /locus_tag="VGC82_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY0422027.1"
                     /translation="MADPTRESAFDLLTAILERHRTLEDALASLPPQDARDRAAAHRL
                     AAAVLRRMGTLDAVLEPFLRKEPPEPVRNVLRIGAAGLLLLETPEHAAVATAVALAKR
                     RGLAPFAGLVNAVLRKVATAGSDALGDLNPVRLDTPAWLWTSWGSAARAIALAHQTEA
                     PLDVTLKPGSVPPKGGVLLPTGSVRFPAGTPVSDIPGFAEGDLWVQDAAAALPARLLA
                     AQPNERVADLCAAPGGKTAQLAAAGAAVTAIERDAGRMERLTANLKHWRLHADVINAD
                     ATAWRPPVPFDAVLLDAPCSATGTIRRHPDVARIKRPRDVQTVTQAQDKLLQAAAGML
                     RQGGRLIYAVCSLQPEEGPPRIAAAAKFGLRHDPFSADELAALPEARTRDGFLRTHPG
                     LWPERGGMDGFFAARLIKA"
     gene            4342..5013
                     /gene="rpe"
                     /locus_tag="VGC82_01745"
     CDS             4342..5013
                     /gene="rpe"
                     /locus_tag="VGC82_01745"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY0422028.1"
                     /translation="MDVSESRHVTIAPSLLAADFGRLRDEVNAIEAAGADWLHLDIMD
                     GHFVPNISFGPTVLGALRKHTKLPFDVHLMIAPVDPFLDAFIDAGADHILLHPEAGPH
                     LHRSLQHIRNAGKKAGIVLNPATPPEAITWALDLTDIVLVMTVNPGFGGQSFLPSQLP
                     KIGRLRQMIDAADHPIALAVDGGVTKKTAPAILAAGADTLIAGTSVFRAPDYAIAISD
                     LRGGA"
BASE COUNT          965 a         1606 c         1565 g         1007 t
ORIGIN      
        1 cgcgacccga accctttgcc cgaacttatt gtcggcaagg tgaacgccgg gaagtgttct
       61 tcacatttat gggcaggtgt ctccacccca tgtcagagca acaatcgcaa gccaacaatc
      121 gccagcccaa caatcgtttg acgtttttac gtcaatacaa caggcctcat cggccttatt
      181 tgaagtttga cggtcgcttg gcagcttagc cgtccggata gtttatgtga aatgaacttg
      241 acaacaaact tgtaatttca ccgcagatcg cacggtcacg gatctgtaga attatgaatt
      301 ggtcgaatag cctcaccgga tactcgatct agtgatgacg gttttatacg gatccgtgtt
      361 aatatcttga aggttccgtt ccgatgggtt tcatatgcgg tatcgaacgc attcaaacca
      421 acggcgcggg tccgcaacga tggcacacgt ccagggtcgc ttcgacaaga ggttatcatg
      481 ccggaagtta tctttaacca tgtcaatcag tttgaacttc tggccgcact ggccgacgcg
      541 gagtgggaga tgtacgaatt aggttcgtgc ttgaatattc ccgggacatt gcgcctccgg
      601 ttactgcagg ccgccgaacg tgcgcgccaa ctgatcgacc cggaggatga aatcggcgtg
      661 tatttaatcc cggacagggg catgacccta cgtttgcctt gatcctcgac aaatcatctt
      721 cagcgcctac tttcttgaac tgacggatct ttaactgatg ggtctcttct cgccaactta
      781 tttggtagcg agtcatggtg acgattaaaa attattctac cggcgaataa tgataataag
      841 aaccacaagc tgaacttaca aacttcggtc cagttacgat tagctgctcc aattggtgca
      901 cgatcaggcc acctcttttt gatgggcgac ggccaaccat ccgtgcctgc cggatttagg
      961 cctgtgccag atttagcacc gcaagccata catggtggac cttcccacca tgacggatct
     1021 cccgggacgc gctcgacaat ctgttgacgg ccagcaccgt cttgctcgtg gccggactcg
     1081 gattgctggt agccgtccaa cttgatacgc gccggtcagc tcaacttgac cagaagatag
     1141 caacgaacga tgacgatcgg aaccaaccct ccaggaggtc tggccatggc gcacagaaat
     1201 agcgctcatt cgaagaattt ctcgactgct cccgcgccgg gccgctatca tgccgtcgtg
     1261 tggctcgaca gccgacaggc gagagtcact tatgtcagca gggatgacag tgaggaggga
     1321 acggtgcggc gcgtcgatcc accgtgtcaa ttacatatcg aagcggcaag aacctcatcg
     1381 aagcgcgcgg ccgatgcgcc cgcattctat cgcgatctcg ccgaggcgtg cgacaaagcc
     1441 aaggccgtgc tgctgatggg cccctcaacg gcgaaggaag acttcctgag gtatctgcat
     1501 cagcattctc ccgcgacctt cgagcttgtt tcaagagccg cgggtgactc gccgtccgct
     1561 actcgctgaa gccctgcgta cgcttccagc cgctgacgtt tgggcggcct gtggtcaatc
     1621 gcgcggtcgg cagccgcttg agccacccgt tcatagcttt tcgccaggtg cggattgtaa
     1681 attactggaa tcctcccgcg ctatgtgctg aatcagccct gtattgcctt ggggaacatt
     1741 gcacgtgcgg ataacgatga ttggcggcgg ctacgtgggt ttggtgtccg gcgcgtgttt
     1801 tgccgagttc ggcaccgatg tcacggtcgt ggaaacagat ccgcgcaaac tatcggcgct
     1861 gcgggatggc cggatgccga tctacgagcc aggcctggaa caacttgtcg ccgaaaacgt
     1921 cagggcgggc cggctctcct tcaccagcga gctggccgcg gcggtcgatg ggaccgaagc
     1981 cgtctttatt gccgtcggga ccccgacccg gcgcggcgat gggcatgcgg atcttaccta
     2041 cgtctatgcg gcggcggagc aggtggccaa tgcgctgacc ggctacgccg tgatcgtcac
     2101 caaatcgacc gtcccagtcg gcaccggccg ccgcatccag gaaattgtcg gcgccacacg
     2161 ccctgatctg gatttcgatg tcgcatcgaa ccccgagttc ctgcgtgaag gcaatgcgat
     2221 cggcgatttc atgcgcccgg accgggtggt gatcggcgcg gagtcggagc gttcgaggga
     2281 gatcatgcgc cgcctgtacc ggccgctata cttgatcgag gcgcccattg tcttcacctc
     2341 aatcgagaca gccgaactga ccaaatacgc cgccaacgcc ttcctggcga tgaaggtcac
     2401 gttcatcaac gaaatggccg atttgtgtga aaaagtcggt gccgacgtgc acgacgtggc
     2461 gcgcggcatc ggcctggacg gacgcatcgg ccgcaaattc ctgcatcccg ggccgggcta
     2521 tggcggctcc tgctttccca aggacacgct ggcgctggtt cgcacggcac aggactacgg
     2581 cgcaccatcc cgcctggtcg agactgtcgt cgcggtcaac gaggcgcgca agtcgagcat
     2641 ggcgatgcgc gtgatcaatg cgtgcggcgg ttcggtacgc ggtaaaacta tcgcggtact
     2701 cggcctgacc ttcaaacccg aaacagacga catgcgggat tcgccgtcgc tgccgatcgt
     2761 ctcccgtctc gccggcgacg gcgcgacgat ccgcgtcttc gatcccgagg gcatggagca
     2821 ggctcggccg ctgttgcccg agagcgtgcg ttactgcgcc gatccgttcg aagctgtcgc
     2881 tggcgcggat gccctggtgc tgattaccga gtggaacgaa ttccgagcga tgtcgccaag
     2941 ccgtcttgcc gaaacgatgc gcggccgggt gatggtggat ctccgcaacg tttatgatcc
     3001 ggtcgccatg cggcaagccg gctttgaata ccatggtatt ggccgagcgg tgctgccggc
     3061 tgatggctga cccgacgcgt gagtctgcgt ttgacctgct gacggcgatc cttgaacggc
     3121 accgcacctt ggaggacgcc ctggcttcgc tgccacccca ggatgccagg gaccgcgccg
     3181 ccgcccatcg gctggccgcc gcggtgctgc ggcgaatggg caccttggac gcggtgctgg
     3241 agccattcct gcgcaaggaa ccgccggaac cggtccgcaa cgtgttgcga attggcgccg
     3301 ccggattgct gctgctggaa acgcccgaac acgccgcggt ggccacggcg gtggcattgg
     3361 ccaagcgtcg aggcctcgct ccgttcgctg gtttggtgaa tgccgttctg cgcaaggttg
     3421 cgacagcggg ttcggatgcc ctcggcgatc tgaatccggt gcggctcgat acgccggcgt
     3481 ggctctggac atcgtggggc tccgccgccc gcgccatcgc tttggcgcat cagaccgagg
     3541 caccgctgga tgtaaccttg aagcccggaa gcgtgccgcc caaaggaggc gtattgctgc
     3601 ccaccggttc ggtgcgcttc ccggcgggaa cgccggtttc cgatatcccc ggattcgcgg
     3661 aaggcgacct ttgggtacag gacgccgccg ccgcgctgcc ggcacgcctg ctggcggcgc
     3721 agccaaacga gcgcgtggcc gatctttgtg cggcaccagg cggcaagacg gcgcaacttg
     3781 ccgcggcagg cgcggccgtt acggcgatcg agcgcgatgc cggccgcatg gaacgcctca
     3841 ccgccaacct caagcattgg cgcctacacg cggatgtgat caacgccgac gcgaccgcct
     3901 ggaggccgcc cgtcccgttc gacgccgtgt tgctggacgc cccgtgcagc gctaccggca
     3961 cgatccggcg tcatcccgac gttgcccgca tcaagcgacc aagggatgtg cagaccgtga
     4021 cccaggctca ggacaagctg ctgcaggccg ccgccggcat gcttcggcaa ggtggccgcc
     4081 tgatctacgc ggtttgctcg ttgcagcccg aggaaggccc cccgcgcatc gccgcagccg
     4141 ccaaattcgg tttgcggcat gacccgttca gcgcggatga actggccgcg ttaccggaag
     4201 cccgtacccg tgacgggttc ctccggaccc accccggact atggccggag cgcggcggca
     4261 tggacggatt cttcgccgcc agactgatca aggcctgatg ttgccttgtg tgacatcggc
     4321 cacggtgtct aaaaacatgc catggatgtt tccgagtcgc ggcacgtcac gatcgccccg
     4381 agcctgcttg ccgccgactt cggccggctg cgggacgaag tcaatgctat cgaagcggca
     4441 ggagccgatt ggctacacct ggatatcatg gacgggcatt tcgttcctaa catcagcttt
     4501 ggccccacgg tcctgggcgc tttacgcaag cacacgaaac taccgttcga cgttcacctg
     4561 atgatcgccc ccgtcgatcc ctttctggat gcattcatcg atgccggggc cgaccacatc
     4621 ctgctgcacc cggaagctgg gcctcatctg catcggtcgc tgcagcacat ccggaacgcc
     4681 ggcaaaaagg ccggcatcgt gctgaatccg gcaacgccac ccgaggcgat tacctgggcg
     4741 ctggacctga ccgacatcgt gttggtcatg acggtaaatc cgggcttcgg cggccaaagc
     4801 ttcctgccct cccagttgcc gaaaatcggg cggctacggc agatgatcga tgccgccgac
     4861 cacccgatcg cactggcggt tgacggcggc gtcacgaaaa aaaccgcgcc cgcgattttg
     4921 gccgccggtg ccgatacctt gatcgccggc acctcggtgt tccgtgcccc cgactacgca
     4981 atcgcgatct cggatctccg aggaggcgca tgaacgtggc agctccaaga acctggatgc
     5041 gtggcgcgcg gcgagcgatg gcgcgcttgc caagccttcg catgggacgc gtacccgatg
     5101 cccccgggct gtcggtccgc gatccctggc cgggcgatcc cgc
//
LOCUS       DASWQN010000109         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_540349, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000109 DASWQN010000000
VERSION     DASWQN010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540349"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..865
                     /locus_tag="VGC82_01750"
     CDS             269..865
                     /locus_tag="VGC82_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY0422029.1"
                     /translation="MKIGIVSDIHCNAIGLQLALDAMGHVDEVLCLGDAIFEYQFSND
                     VVRLLRDRGAHIIQGNHEEVFLSSAGSRARERPGLDPDLIAFLAEQPLRKELLVGGKK
                     LLMVHSTPWEPRGEYVHPHSTKLVRFAETDAHYVLYGHTHVQVVRRVGRVLVINPGSA
                     GDGRDSNNDRQLSCATLDTATDEVEVLNFPDPRFAPKP"
     gene            complement(906..2474)
                     /locus_tag="VGC82_01755"
     CDS             complement(906..2474)
                     /locus_tag="VGC82_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422030.1"
                     /translation="MPRTIFVLAACLCLSFPPVAVAQTLHVGVGGAFTSIDPHYHNVG
                     PNNVLTEHVFDRFLHFDPNFHVVPGLAVSWTPVDDRTWEFKLREGVTFHDGTPFTADD
                     VVFTFGRIPLVVNSPSSFNFAVKPIDRIEVVDPQTIRLHTQTPSPLLAYNLAAVRIVS
                     RKNGQGAGTGDYNALKAAIGTGPYRVTEFAAGERVRLERNDEWWGKKPLWTTVDYRSI
                     VNDASRNAALQAGEVDIIDQVPTRDVEDLKKNPKVVVLSAPGQRLIYLSPDIGQPRNA
                     FATDIAGLPLPVNPLRDVRVRKALSLAINRAGIRDRIMDGFSVPTGQLMPLGASGYEP
                     SLAPDPYEPEAAKKLLAEAGFPHGFGLTLHGPNNRYVNDSKIMEAVAQMWTRIGVKTT
                     VDAMPSATFFSRVLRREFAIRLTGWASDTGEASSDLTELLASSNPAKGRGAVFDPQRY
                     ANDNVDGIVEQALATLDLDKREVLYREAERRAMPDYPIIPLHHQVNVWAVRKGILFHP
                     RMAEGIRAWDVQPE"
     gene            2655..>2871
                     /locus_tag="VGC82_01760"
     CDS             2655..>2871
                     /locus_tag="VGC82_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422031.1"
                     /translation="MVDHKYVMAAPCALSLRLEKLRARLPPGVDVSWPSLARPLTTEV
                     VGQNFGQVDKWKVCLKAATMPQIRSEHG"
BASE COUNT          569 a          891 c          866 g          545 t
ORIGIN      
        1 catcagcagt ctatattaag ggaattgccc gctattttgc ctgtaatggt atctatttta
       61 aaattatgct tgcgaataga tgctgcaggc gctgatggct caaaaattac attgcgttac
      121 attacgaaac gagatcgtga cgatagccgg gttcgagcac cctctcgtcg tccgccgccc
      181 accgtccgcc cccgatacgc attgaaacca gacccacctt ctttatggtt cggcagccgg
      241 aggcggtacc ggaagggaga atttttgcgt gaagattggt attgtctcag acatccattg
      301 caacgccatt gggctgcaac tcgcactgga cgcgatgggg catgtcgatg aagtactttg
      361 cctcggggat gcgatcttcg agtaccagtt ctccaacgac gtggtacggc tgcttcggga
      421 tcgcggcgcg cacatcatcc aaggcaacca cgaggaggtt ttcctttcct cggcgggaag
      481 ccgggctcgt gaacggccgg gactggaccc ggacctgatc gcattcctgg ccgaacagcc
      541 gctccgcaag gagttgctgg tcggcggcaa aaagctgctg atggttcact cgacgccgtg
      601 ggagccgcgc ggagagtacg ttcatccgca cagcacgaag ctggtccgct tcgccgaaac
      661 cgacgcgcac tacgtattgt atgggcacac ccatgtgcag gtggtgcgcc gggttggacg
      721 cgtgctggtg atcaacccgg gatccgcggg agacggacgg gacagcaata acgaccgcca
      781 attgagttgt gcaactttgg acacggcaac cgatgaagtg gaggtgctga attttcccga
      841 tccgcgcttc gcgcccaagc catgacgccg ggtaaaaccg cttccccgcg cggcggacgc
      901 gaacgttatt ccggctggac atcccaggcg cggattccct cggccattcg cggatgaaac
      961 aggatgccct tgcgcaccgc ccacacattc acctgatgat gcagcgggat gatcggatag
     1021 tccggcatcg ccctccgttc ggcctcccgg tacagcacct cgcgcttgtc caggtccagc
     1081 gtcgccagtg cctgttcgac gatcccgtcc acattgtcgt tggcatagcg ctgcggatcg
     1141 aataccgcac cacgcccctt cgcggggttg gaggaagcga gcagttccgt aaggtcactg
     1201 ctggcctccc cggtgtcgct cgcccacccg gtcaggcgga tcgcgaactc ccgccgtagc
     1261 acgcgactga aaaacgtcgc cgacggcatg gcatcgaccg tcgtcttgac gccgatccgg
     1321 gtccacatct gggcgactgc ctccatgatt ttcgaatcgt tgacataacg attattcgga
     1381 ccatgcagcg tcaggccgaa accgtgcgga aaccccgcct cagccagcaa ttttttcgct
     1441 gcctccggtt catacggatc gggcgccagc gaaggttcgt atccgctggc gccaagcggc
     1501 atcaactggc ccgtcgggac ggagaacccg tccatgatcc ggtcacgaat gccagcgcga
     1561 ttgatcgcca gcgacagcgc cttccgcacc ctgacgtccc gcagcggatt gaccggcagc
     1621 ggcaggcccg cgatgtccgt ggcgaatgcg ttccgcggtt ggccgatatc cggcgaaagg
     1681 tagatcaggc gctggccggg cgccgacagc acgacaacct tggggttctt tttcagatcc
     1741 tccacgtcgc gagtcggcac ctggtcgatg atatccactt cgcccgcctg cagtgcggcg
     1801 tttcgcgacg cgtcgttgac gatcgagcgg tagtccaccg tcgtccagag cggcttcttg
     1861 ccccaccatt cgtcgttacg ctccagccga acccgctccc ccgcggcaaa ctccgtcact
     1921 cgatacggac cggtgccgat cgccgccttc agcgcgttgt agtcgcccgt gccggcgccc
     1981 tggccgttct tgcgggacac gatccgcacc gccgccagat tgtaggccaa caatggagat
     2041 ggcgtctggg tgtgcagccg aatggtttgc ggatcgacca cctcgatccg gtcgatcggc
     2101 ttcaccgcga agttgaagct ggacggactg ttcacgacca aggggatgcg cccaaaggtg
     2161 aacaccacgt catcggcggt gaacggcgtg ccatcgtgga acgtgacgcc ctcgcgcagc
     2221 ttgaactccc acgtcctgtc atcgaccggg gtccaggaca ccgccaggcc cggaaccaca
     2281 tggaagttcg ggtcaaagtg caggaaccgg tcgaaaacgt gttccgtcag gacattgttc
     2341 gggccgacat tgtggtagtg cgggtcgatc gaggtgaatg ctccaccaac cccgacatgc
     2401 agcgtttgcg ctacagccac cggcgggaac gagaggcaaa ggcaggcggc aagaacaaag
     2461 atcgttcgag gcatgaggca atcccgattt ggctgcttac aggaccatgc cgaggttttc
     2521 gccgcggtgc cagcacctga ccaaggcttc gctacgaaac aaccgctttg ggcgccgtgt
     2581 ccaaacccga tggggccggc gaacgcctca cagagacaag aacatgacgc tccagcggtg
     2641 cctggcaccg ggtagtggtt gaccacaaat acgtcatggc cgcgccttgt gcgctttcac
     2701 tccgactgga aaagctgcga gcccgtttgc cgcctggtgt cgatgtgtca tggccgagct
     2761 tggcgcggcc actcacgaca gaggtggtcg gccaaaattt cggacaggtc gataagtgga
     2821 aagtctgcct gaaagcggcg acgatgccgc agatcaggag cgagcatggc a
//
LOCUS       DASWQN010000110         5377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2255744, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000110 DASWQN010000000
VERSION     DASWQN010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2255744"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            554..1597
                     /locus_tag="VGC82_01765"
     CDS             554..1597
                     /locus_tag="VGC82_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY0422032.1"
                     /translation="MFSRLLGFMSADMAIDLGTANTLVYVKGRGIVLAEPSVVAIADV
                     RGKKQVIAVGEEAKMMLGRTPGYITASRPLRDGVIADFEVAEEMIKHFIRKVHNRRRF
                     ASPLIIVCVPSGSTAVERRAIQESAESAGARRVQLIEEPMAAAIGAGLPVTEPSGSMI
                     VDIGGGTTEVAIISLGGIVYSRSVRVGGDKMDEAIISYMRRNHNLLIGETTAERIKMD
                     IGAAASLDNGDEGPYREVRGRDLMNGVPREVVVSQREIADSLMEPVSAIVEAVKVALE
                     NTPPELAADIVDRGIVLTGGGAMLYRLDQVLRDATGLAVAIAENPLQCVALGTGRALE
                     ERKRLSNVLTSMY"
     gene            1649..2509
                     /gene="mreC"
                     /locus_tag="VGC82_01770"
     CDS             1649..2509
                     /gene="mreC"
                     /locus_tag="VGC82_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY0422033.1"
                     /translation="MIRLSIQARQALVRLTLPVLIVFSFAIMLIGKADTLLAERARTA
                     LADGLVPIYAALANPLGHLHATIAETAALWNMREENARLRAENEKLRRWQSVALALDA
                     ENQRMKASLRWIPDPDVSFVTARVVADAGGVYAKAVLLSVGPNHGIRKGEIAVDERGL
                     VGRITEVGSRTARVLLLTDLNSRIPVILETSRAHAILAGTNGTRPRLQYWPEGVVPLE
                     GERIVTSAEANAFPANLPIGTVRYTNSGAPEVEPAAMLQKLEVVRIFDYGLNGVTAPE
                     ALSRPTPGRH"
     gene            complement(2578..2931)
                     /locus_tag="VGC82_01775"
     CDS             complement(2578..2931)
                     /locus_tag="VGC82_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422034.1"
                     /translation="MTSSQIANEATPGHGPKNNPSPDEAAMRQRLPHGQGQAMDSQQR
                     EHPDSERQVTQKVHRQAKQKDARRGQMIRAIQRPEPDAAQGDSGQQRRLFGNAQRRLR
                     QQHQQDGDAARNADP"
     gene            2930..3064
                     /locus_tag="VGC82_01780"
     CDS             2930..3064
                     /locus_tag="VGC82_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422035.1"
                     /translation="MLLSFRLLSPYPALFMAILTVAIFPILAVPFAAAHRLIADPATA
                     "
     gene            3144..4982
                     /gene="mrdA"
                     /locus_tag="VGC82_01785"
     CDS             3144..4982
                     /gene="mrdA"
                     /locus_tag="VGC82_01785"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY0422036.1"
                     /translation="MFTRRALLLMGGQVAVFGVLGARLYQVQVREGARYTMLADENRL
                     SARMIAPPRGRVLDRFGEVLAGSSTNWRAVLIAEQVNDVAATLDAFSALLPLGEYKRG
                     RIDRDLHRKRKFVPIMVREFLTWDEMAAIGVNAPDLPGIVADAGTTRHYPFAGKLAHV
                     VGYVAPPNEADVADEPLLGLPGLRIGRAGMEKYHDLGLRGRAGAVQLEVNAVGRVIRE
                     LDRQEGVPGEDVGLTLDSGLQLSVLSTLRDESASAVVMDCRNGEVLAMVTNPSFDPSV
                     FNSGVSQAQWLDWTRDRRTPLINKVTSGLYSPGSTFKMAVALAGIESRAIAPTDRFEC
                     PGFLDIGDARFHCWRKDGHGKQDLRGGLKHSCDVYFYEVARRTGIDRIAAMSNRLGLG
                     IELELDLPNQRAGLIPTREWRASHGHAWNMGDTIVSGIGQGFVQVTPLQLATYASRIA
                     TGRLVQPHLTRKLAGVMQPGAEPDDWPVLGVPEKLLHMVREGMWAVVNEPGGTAPLAR
                     LADPQVQLAGKTGSAQVRRVSRELRESGHFDSEKLPWEYRPHALFVAFAPYEAPRYAL
                     SVVIEHGNAGAAAAAPMAREIMTEVLRRDPANRTDAPPAQVAEARS"
     gene            4982..>5377
                     /locus_tag="VGC82_01790"
     CDS             4982..>5377
                     /locus_tag="VGC82_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY0422037.1"
                     /translation="MDRRLWREPSFDLMGKVLRINWLYVILLCCLAAIGYAALYSAAG
                     GLPEPYATRHAERFAIGVLIMLVIGLIDIRFIAKLAWPAWIGGVVLLVLVLRMGHVGK
                     GAQRWLEIGGLQLQPSELMKLALVLALASW"
BASE COUNT          905 a         1709 c         1794 g          969 t
ORIGIN      
        1 cttaatgcag gctcccgcat ccccggatgc acgcctcgca gcagacacgc agtcctcagc
       61 ggggcaacaa tcaaatcaac ggccgatgtt cgtcggaagg gcaaccaccg tatttcgacg
      121 ctaccgggtg acggccccac ccactgcgcc gaggccgcca ccgaccaaag cgccggttgc
      181 cggcctgcct cccgtcaccg ccgagacgcc ggcacccgcg cccgcaccca acaggccgcc
      241 ggtcacagcg cgagtaccgg tgctgctacc gcagccagcc agccctacag atgcgctgac
      301 gagcaagaca aaagcggcta atcgcatcga attcctccct tgagtgctat tttcacatgg
      361 gtctgcattc gcccgatttc ccgccggaat acgggcaaat gtgcggcttg ccatcaaacc
      421 attgtcaaga aacccttgtt cagccgacac gtcggggcta agccgaaccc tccccgaacg
      481 ccccctccgt gtccggccaa cactcatccg agccgccgcc tgctcccggc gcttcacgac
      541 aaaggttttc cgaatgttct ctcggctgct cggcttcatg tcggccgaca tggccatcga
      601 cctcggcacc gccaatacac tcgtctatgt caagggccgg ggcatcgttt tggccgaacc
      661 gagcgtcgtg gcgatcgccg atgttcgcgg caaaaagcag gtcatcgcgg tgggcgagga
      721 agccaagatg atgctcggcc gcacgcccgg gtatatcacc gccagccgtc cattgcgcga
      781 cggggtgatc gcggacttcg aagtcgccga agagatgatc aagcacttca tccgtaaggt
      841 gcataaccgg cgcaggttcg cttccccact gatcatcgtt tgcgtgccct ccggttcgac
      901 cgccgtcgaa cgccgggcca ttcaggaaag tgccgaaagc gccggcgccc gccgggtgca
      961 actgatcgag gaaccgatgg ccgcggcgat cggcgccggc ctgccggtca ccgaaccatc
     1021 cggcagcatg atcgtcgata tcggcggcgg caccaccgaa gttgcgatca tctcgctcgg
     1081 cggcatcgtt tattcgcgca gcgtgcgtgt cggcggcgac aagatggacg aggcgatcat
     1141 cagctatatg cgccgcaacc ataacttgtt gatcggtgaa accacggccg aacggatcaa
     1201 gatggacatc ggcgccgccg cctcactcga taacggcgac gaaggcccgt accgggaagt
     1261 gcgcggccgc gatctgatga acggcgtccc gcgggaggtc gtggtcagcc agcgtgagat
     1321 cgccgacagc ctgatggagc cggtttcggc gatcgtcgaa gcggtcaagg tggcgctgga
     1381 aaacacgccg ccggaactgg ctgccgacat cgttgatagg ggcatcgtgc tgaccggcgg
     1441 cggtgcgatg ctgtaccggc tcgatcaggt gcttcgcgat gcgaccggcc ttgcggtcgc
     1501 catagcggaa aacccgctgc aatgcgtggc actgggcacc ggacgcgcac ttgaggaacg
     1561 aaagcgtttg agtaacgtgc tgacgagcat gtattgatat ctccaacccc gttcggcgcc
     1621 ggcaagcgcc cgcaggctaa aggaccgcat gatccggcta tcgatccagg cacgccaggc
     1681 gttggttcgg ctgaccctgc cggtccttat cgtcttttcg ttcgccatca tgctgatcgg
     1741 caaggcggat acgctgctgg cggaacgggc gcggacggca ctcgccgacg gcttggtgcc
     1801 gatctatgca gcgctggcaa atccgctcgg gcaccttcat gccacgatcg cggaaaccgc
     1861 ggcgctgtgg aacatgcgcg aggaaaacgc ccgccttcgt gcggagaacg aaaagctgcg
     1921 gcgctggcaa tctgtcgctt tggcgctgga cgcggaaaac caacgcatga aagccagcct
     1981 gcgctggatc ccggatccgg acgtgtcgtt cgtcaccgcc cgtgtcgtgg ccgatgccgg
     2041 cggggtttat gccaaggccg tgctgctgtc cgtcggcccc aaccatggca tccgcaaggg
     2101 cgaaatcgcg gttgacgaac gcggtctggt cggccgcatc accgaggttg gatcgcgcac
     2161 cgcgcgcgtg ttgttgctca ccgacctcaa cagccggatc ccggtcatcc tggaaacttc
     2221 gcgcgcccac gcgatcctgg cgggcaccaa cggcacacgg ccccggttgc agtactggcc
     2281 cgaaggcgtt gtgccgctgg aaggcgagcg gatcgtgacc agcgccgagg cgaatgcgtt
     2341 tccggccaac ctgccgattg gaacggtgcg ctataccaat agcggcgcac ccgaagtcga
     2401 gcccgcggcg atgctgcaga aactggaagt cgtgcgaatc ttcgattacg ggctgaatgg
     2461 cgtgaccgcg ccggaagcgc tttcgagacc gacccctggc cggcactgat ggcccaaatc
     2521 gaccggcgtc cgggcatccg gccacgcctc agtcagggcc gccgcctcga tatcgcatca
     2581 cgggtcagcg ttccttgcgg cgtcaccgtc ctgctgatgt tgctgacgca ggcgccgctg
     2641 ggcattcccg aacaggcggc gttgctgccc gctgtcacct tgtgctgcgt ctggttctgg
     2701 tcgctgtatc gcccggatca tttgcccgcg ccttgcgtct ttttgcttgg cctggcgatg
     2761 gaccttttgg gttacctgcc gctcggagtc ggggtgttca cgctgctggc tgtccatggc
     2821 ctggccctgg ccgtgcggca accgttgtcg catcgcggct tcgtctgggg atggattgtt
     2881 tttggggccg tggccgggcg tcgcctcgtt ggcgatctgg ctgctggtca tgctgctgtc
     2941 gttccggctg ctgtcgccat atccggcgtt gttcatggcg atcctgaccg tcgcgatctt
     3001 cccaatcctg gcagtcccct ttgccgccgc gcatcgccta attgccgatc cggcgacggc
     3061 ctgatgagga acgggaatga ccgccgcgca tttccggccg tgaaacgccg ttcgccgtga
     3121 aacgagacgc ggaacggacc ggcatgttca cccgccgggc gcttctgctg atgggcggcc
     3181 aggtcgccgt gttcggtgtc ctgggtgccc ggctgtatca ggtgcaggtg cgcgagggcg
     3241 cccgctacac catgctcgcc gatgagaatc gcctgagcgc gcggatgatc gcgccgcccc
     3301 gcgggcgcgt tctcgatcgc tttggcgaag ttttggccgg cagcagcacc aactggcgcg
     3361 ctgtcctgat cgccgagcag gtgaacgacg tggcggcgac gctggatgct ttctcggcgc
     3421 ttctgccact cggcgagtac aaacgcggcc gcatcgaccg cgacctgcac cgcaagcgca
     3481 aattcgtgcc catcatggtc cgggaattcc tgacctggga cgagatggcg gcgatcgggg
     3541 tcaacgcccc ggatctgccg ggcatcgtcg ccgatgccgg caccacgcga cactatccgt
     3601 tcgccggcaa actcgcccat gtcgtcggct atgtcgcgcc gccgaacgag gctgatgtcg
     3661 cggacgaacc attgctggga ttgcccggcc tgcgcatcgg ccgtgcgggc atggaaaaat
     3721 accacgacct tggattacgc gggcgcgccg gggcggtgca actggaggtg aatgcggtcg
     3781 ggcgcgtgat ccgggagttg gaccgccagg agggcgttcc gggggaggat gtcggcctga
     3841 cgctcgattc cggcctacaa ctatcggtgc tgagcacgct gcgcgacgag agcgccagcg
     3901 cggtggtcat ggactgccgg aacggcgaag tgctggcaat ggtcaccaac ccgtcgttcg
     3961 atccgtccgt gttcaactcc ggcgtgtcgc aggcgcagtg gctggactgg acgagagacc
     4021 gccgtacgcc gttgatcaac aaggtgacgt ccgggcttta ctcgcccggg tctactttca
     4081 aaatggctgt cgcgctcgct ggcatcgagt ccagggcgat cgcaccgacc gaccggttcg
     4141 agtgtccggg cttcctcgat atcggcgacg cgcggtttca ttgctggcgc aaggacggtc
     4201 acgggaagca ggatctgcgc ggcggcctga agcatagttg cgacgtgtat ttctatgagg
     4261 tcgcacggcg caccggcatc gaccggatcg cggcgatgtc gaaccggctg gggttgggga
     4321 tcgaactgga gctggatttg ccgaaccaac gtgcgggcct gatcccgacg cgagagtggc
     4381 gggccagcca tggtcacgcc tggaacatgg gcgacacgat cgtcagcggt atcggacagg
     4441 gtttcgtcca ggtgacgccg ttgcagctcg cgacctatgc ctcccgtatc gccaccgggc
     4501 ggctggtgca gccgcatctt acccgcaagc tggcgggagt gatgcagcct ggggcggagc
     4561 cggacgattg gccggtgttg ggcgtacccg aaaagctgct gcacatggtg cgcgagggta
     4621 tgtgggcagt ggtaaacgag ccaggcggca cggcgccgct tgcccgcctg gccgatccgc
     4681 aggtgcaact ggccggcaag accggttcgg cgcaggtgcg gcgcgtctca cgggagctgc
     4741 gggaaagcgg gcacttcgac agcgagaagc tgccctggga ataccgtccg catgcgctgt
     4801 tcgtcgcctt cgcgccgtac gaagcgcccc gctacgcgct gtcggtggtg atcgagcacg
     4861 gcaatgccgg tgcggcggcg gcggcaccaa tggcgcggga aatcatgacc gaggtgctgc
     4921 ggcgcgatcc cgccaatcga accgacgcgc cgccggcgca ggtggcggag gcgagatcct
     4981 gatggaccgt cgattgtggc gcgagccgtc gttcgacctg atgggcaagg tgctgcggat
     5041 caactggctt tatgtaatcc tgctgtgttg cctggccgct atcggttatg ccgcgctgta
     5101 cagcgcggcc ggcggcttgc ccgaaccgta tgcgacgagg catgccgaac ggttcgcgat
     5161 cggcgtcctt atcatgctgg taatcggact gatcgatatc cggttcatcg cgaaactggc
     5221 ttggccggcg tggatcggtg gcgtcgtcct gctggtcctg gtgctgcgga tgggccacgt
     5281 cggcaagggt gcgcaacggt ggctggaaat cggcgggctg cagctgcagc cttcggaact
     5341 gatgaagctg gcgctggtgc tggcgctggc gtcctgg
//
LOCUS       DASWQN010000111         8050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1596053, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000111 DASWQN010000000
VERSION     DASWQN010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596053"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VGC82_01795"
     CDS             <1..521
                     /locus_tag="VGC82_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422038.1"
                     /translation="AAIGGDGIAWTGGGGITCEGGESEAVVAGAGTAGPGGAGTASAA
                     DVGRDGLPSAIAEGPGAIAEGIAAVGDAAVAAGGGGGIAAAGGEGTDATGAASGAGGE
                     AIDGRSGVEAAEKGNGLVAGCDEAAKGAPGATGSSPSAIGGVAGRASAWRSEAWRSGA
                     WRSGAWRSGACG"
     gene            554..799
                     /locus_tag="VGC82_01800"
     CDS             554..799
                     /locus_tag="VGC82_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422039.1"
                     /translation="MLRSKFTLQPRYLQLQSSHAVGLIGGLIRRQVGHGMLHERVPLI
                     LYFSQINRRLAIEGEAQEDQAGYPCHGRYSKAQRLFL"
     gene            1012..1503
                     /locus_tag="VGC82_01805"
     CDS             1012..1503
                     /locus_tag="VGC82_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0422040.1"
                     /translation="MPQEFHDQLISFMPKMRVWALALTRNRAAADDLAQDVATKALVA
                     KDCFIPGTNFSAWVHRIMINHFISSVRSKREFTDMENMPEIAVAAAHEDRTALRELGW
                     ALDRLPPDQREALFMIVLQEKSYEDAAAQTGCAIGTLKSRVHRARLQLRAFLIGDETK
                     MAA"
     gene            complement(1695..2648)
                     /gene="egtD"
                     /locus_tag="VGC82_01810"
     CDS             complement(1695..2648)
                     /gene="egtD"
                     /locus_tag="VGC82_01810"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEY0422041.1"
                     /translation="MPNDAEFLPPQTRSDVLGIALAGLTATPKTLPAMLFYDDEGCRL
                     FYQITRLPEYYLTRTETTLLNRIASRVVPDGFRDAVLVEFGGSDESKALILLDRPDTP
                     FATYVSIDVAAPALGALQARLSQSHPGLKVVPIVADFLHPVPLPPLGAPRMGFFPGST
                     IGNLAPAAATAFLAAARTSLGGDAWFLLGADLRKSPKMLLPAYNDAAGVTAAFNLNLL
                     TRLNREAGADFDLGAFRHEAVWNDRESRIEMHLFSTRDQTVVLGGERIAFTEGESIHT
                     ENSYKHTPEKLMDIARSGGWETQQMWQEDAGLFGVFLLRCC"
     gene            complement(2661..3863)
                     /gene="egtB"
                     /locus_tag="VGC82_01815"
     CDS             complement(2661..3863)
                     /gene="egtB"
                     /locus_tag="VGC82_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEY0422042.1"
                     /translation="MDDIGLYPETARASDLSRQFEVVRGAMEALVAELTEEDQCVQSM
                     PDASPAKWHRAHTTWFFEQFVLIPHVPDYRVFDPSFCFLFNSYYNAVGPRHPRPSRGL
                     LTRPPAHQVTRYRQHVDAALHVAIPNLPADVCAIVELGLHHEQQHQELLVTDMLHAFA
                     AHPLVPAMITDWREPLGGGDETVFVESEGGLHRFGFNTDGFCFDNETPRHRAYLQPYA
                     IASRLVRNRDWLGFIQDKGYQTPSLWMSDGWAAVQEAQWSAPLYWRDVDGAWFQAGPG
                     GLAPLLLDAPVRQISWYEADAFARWSQARLPTEFEWEAASTHPSIRETTGHVWQWTES
                     AYRPYPGYRAAPGAIGEYNGKFMINQMVLRGGSLATQPGHSRPTYRNFFHPDKRWQFS
                     GLRLAKDL"
     gene            4018..4887
                     /locus_tag="VGC82_01820"
     CDS             4018..4887
                     /locus_tag="VGC82_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY0422043.1"
                     /translation="MANRPIWRGHLRLALVSCPIALHTVVRASSGLHFHLINPKTGHR
                     IRMVSQDAETEQEVSRQDLIKGFEFEKDRYVLLDDDDFKQARIESSSILTIGKFVDVA
                     TIDPIFFDTSYYVTPDGEAGQDVFVVLREAIQKSRAAALSRVVIAQRERAVAILPSGK
                     GLVCHTLHEPSDLWDAGPLFADIGDMKPEADMVALATQLIERQNGKFQPEDTQDRYEA
                     KLRDVIDAKLKGEGITPEEPSEPDRGNVIDLMAALKASLGPEKSRRGSAAKAPGGGTA
                     KAPAAKKSPRKRA"
     gene            4960..5517
                     /locus_tag="VGC82_01825"
     CDS             4960..5517
                     /locus_tag="VGC82_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY0422044.1"
                     /translation="MANIAGLKIAVLATDGVEQVELTEPVKALKALSAQVQVIAPKGG
                     QIQGMNHDEKGDQLPVDQELASVSPEQFDALVLPGGVTNPDTLRTNPQAVAFVKHFVV
                     ANKPIAAICHGPWTLIEAEGVHGRRMTSWPSLHTDLKNAGAKWEDNSVVVDGNLVTSR
                     KPDDLPDFCREMVKLFEAGQSRKAA"
     gene            complement(5658..7307)
                     /locus_tag="VGC82_01830"
     CDS             complement(5658..7307)
                     /locus_tag="VGC82_01830"
                     /inference="COORDINATES: protein motif:HMM:NF004780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0422045.1"
                     /translation="MRHIATKVLIAGGGPIGLTMAMDLAWRGIPVLVVESRAPGEPPS
                     VKCNHVSARTMEIFRRLGVAQAVRNAGLPADYPNDVAFRTTTTGIELSRIPIPCRADR
                     YTATGGPDTWWPTPEPPHRINQIYLEPVLFEHAKSMQGVRIINRMQLIAFEQHQNGVS
                     ATAADLDGGENLTVSADYMIGADGAHSMVRRAIGARLTGDPMLEQRQSSYIKAPDLLR
                     ATPTKPAWANTSLNPRRSANMFAIDGRETWLVHNYMRPGETFDSVDREHCIRTILGVG
                     PSFPIEILAKEDWTARRMIADRFRDRRVFLCGDAAHIWVPNAGYGMNAGIADAMNLSW
                     MLAGVLKGWASPAILDSYEAERMPITEQVSHYAMDTSTALARQRGGGIPDNIKEPGPE
                     GDAARARVGQDAYDINVGQFCCGGLNFGSFYENSPIIAHDDSAAPEYTIYDFTPSTVP
                     GCRTPHIWLGEGLSLYDAMGADYTLLRFDPQVDVSRMLAAAQNGGVPLVLLDVTTREP
                     VYTEKLVLSRPDQHVAWRGNAAPSDPLALIDRIRGAGPSRP"
     gene            complement(7354..>8050)
                     /locus_tag="VGC82_01835"
     CDS             complement(7354..>8050)
                     /locus_tag="VGC82_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422046.1"
                     /translation="SIAIVNENTDYGTSVGGAIEAEAKKMGIPVSIRVPYSANGADVS
                     TQVLQLKEKNPSVALFVSYASDAILFLRTMKSLDYLPPMIIGDSAGFSDPAFLPAVGG
                     LAKGLVNRSVWSKGPAGSLSNGVNAIYQAKTGNEIGDVSGRCMQAFFVLADALNRAGS
                     ADPAKLREALQATAMPHSAMMVGYRGVRFDETGQNVESATYLTQLQGKDYDTIWPDDA
                     AAAKVNWPMTGWR"
BASE COUNT         1523 a         2506 c         2548 g         1473 t
ORIGIN      
        1 ccgccgcaat cggaggagac ggcatcgcct ggaccggtgg aggcggcatc acctgcgaag
       61 gcggcgaaag cgaagcggtc gtcgccggcg ccggaacagc gggtcccggt ggagcaggca
      121 cagcctcggc tgccgacgtc ggcagggacg gcctgccgag tgcgattgcg gaaggtccgg
      181 gtgccattgc ggaaggtatt gcagcggtgg gcgatgccgc tgtcgccgcc ggcggcgggg
      241 gcggcatcgc tgctgccggc ggagaaggca cagacgcaac cggcgccgcc tccggggccg
      301 gcggggaagc catcgacggc agatctggcg tggaagccgc tgaaaaaggc aatggcctcg
      361 tcgcgggctg cgacgaagcc gccaaggggg cgccgggtgc taccggcagt tcgccatcgg
      421 ctatcggcgg cgtggctgga cgcgcctcgg cttggcgatc cgaggcttgg cgatccgggg
      481 cttggcgatc cggggcttgg cgatctggcg cctgcggttg agcgttcaac acgtcaggct
      541 gtggcaccgg cccttgctgc gcagcaagtt cactctccag ccgcgctacc tccagttgca
      601 atcgagccac gcggtcggcc tcatcggtgg cctgatccgc cggcaagtcg gccacgggat
      661 gctccatgaa cgcgtcccac tcatcctgta cttttcgcag ataaaccgcc ggctggccat
      721 agaaggcgag gcccaggagg atcaggccgg atacccctgc cacggtcgat acagcaaagc
      781 gcaacgttta ttcctctaaa gtatcacgga accgagaaca acgattagcc acctaaacaa
      841 gccgatccgc gtgcactgcg gcacgccgtc acggcatcat accagttaca tctgacgtaa
      901 catgtaacgc gaaccgtcca tcaccgtcct gtcaattgcc ttgcacagcc ctattccgcg
      961 ccacagttgt aaccggcaag ccgtgctcgc atgctcagga ggctccgtcc aatgccgcag
     1021 gaatttcacg atcaacttat ttcatttatg ccaaaaatgc gtgtctgggc cctcgcttta
     1081 acacgaaacc gagcagccgc cgacgatctt gcacaggatg tggcgacgaa ggcgttggtc
     1141 gccaaggatt gctttattcc cggaacgaat ttttcggcct gggttcaccg catcatgatc
     1201 aaccatttca tcagcagcgt gcggagcaag cgcgagttca cggacatgga gaatatgccc
     1261 gaaattgcgg tcgcggcggc ccatgaggac cgcaccgcat tgcgcgaact gggatgggcc
     1321 cttgaccgtc tgccgcccga tcagcgcgag gcgctattca tgatcgtgtt acaggagaaa
     1381 tcctatgagg atgcggcggc gcagaccggc tgcgccatcg gcacgctaaa aagccgcgtg
     1441 caccgcgcca ggctgcagct ccgggcgttt ctgattggcg atgagaccaa gatggccgcc
     1501 taaagcgaaa tccgcgctga tagacgcacg gcttccgctt ccgctttagc cttgttttca
     1561 cgagcagctt tatccggtag gtgatcccac ttggttggat aatgccctgg tgatccgaac
     1621 ctggcgcctg gcttccaggc gtcaggtcaa tcggccacca gatcagcgta cggtaggttg
     1681 tcgccgacga aatttcagca gcagcgcaat agaaacacgc cgaacaggcc ggcatcctcc
     1741 tgccacatct gctgcgtttc ccagcctccg cttcgggcga tgtccatcag cttttcgggc
     1801 gtgtgcttgt agctgttttc ggtgtggatg gattccccct cggtgaacgc gatcctttcg
     1861 ccgccgagaa ccaccgtttg gtctcgggtc gagaacagat gcatctcgat ccgactttcg
     1921 cggtcgttcc aaacggcctc gtgcctgaag gcgccgaggt cgaagtcggc cccagcctcc
     1981 cgattcagcc gggtgagaag attgaggttg aaagccgcgg tgacaccggc ggcatcgttg
     2041 taagccggca gcagcatctt ggggcttttc cgcagatcgg caccgagcag gaaccaggca
     2101 tcgccaccca atgaagtccg tgccgccgcg aggaatgccg ttgccgcggc aggagcgagg
     2161 ttgccgatcg tggaccccgg aaagaacccc atacgcggcg cgcccagcgg cggcaagggc
     2221 accggatgca agaagtcagc aacgatcggc acgaccttca ggccgggatg gctctggctc
     2281 aaccgcgcct gcagcgcacc tagtgccggc gcggcgacat cgatcgacac ataggtggcg
     2341 aacggcgtat cgggccggtc gagcagaata agtgccttgc tttcatcgct gccgccaaac
     2401 tcgaccagga cagcatcgcg gaatccatcc ggcacgacgc gggacgcgat ccggttcagc
     2461 agcgtggttt ccgtcctggt cagataatac tccggcaacc gggtgatctg ataaaacaac
     2521 cggcagccct catcgtcgta gaacagcatc gccggcagcg ttttaggggt cgcggtcaac
     2581 ccggcgaggg caatcccaag cacatccgac cgcgtttgcg gcggcaaaaa ttcagcatcg
     2641 ttcggcacgt cagcctccgg ttaaaggtct tttgccagac gaaggccact gaactgccat
     2701 cgcttgtcgg ggtgaaagaa attccggtaa gtcgggcggc tgtgtccggg ctgtgttgcc
     2761 agcgagccac cccgcaacac catctgattg atcatgaact ttccgttgta ctctccgatt
     2821 gccccggggg ctgctcggta tccggggtaa ggccggtagg cgctctcggt ccactgccag
     2881 acatggcccg tcgtttcgcg aatcgatgga tgggtactgg cggcctccca ctcgaattcg
     2941 gtaggcaacc gggcttggct ccagcgcgcg aaagcatccg cttcgtacca gctgatctgg
     3001 cgaaccgggg catccagaag cagcggcgca agcccgccgg gaccggcctg gaaccaggcc
     3061 ccatcgacgt cgcgccaata caacggcgcc gaccattgtg cctcctgcac cgctgcccat
     3121 ccatcggaca tccacagcga cggggtctgg tagcctttgt cctgaatgaa gccaagccag
     3181 tcgcggttgc gcaccagacg agacgcaatg gcgtacggct gcaggtaagc ccgatgccga
     3241 ggcgtttcgt tatcgaagca gaatccatcc gtattgaatc cgaaccggtg caggccacct
     3301 tcggactcga cgaacacggt ttcgtcgcca ccgccaagcg gttcacgcca gtccgtaatc
     3361 atcgccggaa ccagcggatg ggcagcgaaa gcatgcagca tatcggtgac cagcagttcc
     3421 tgatgctgct gttcgtgatg cagcccaagc tcgacgatgg cgcagacatc cgctggcaga
     3481 ttcgggatcg cgacgtgtag tgcggcatcc acgtgctggc ggtatcgcgt cacctggtgc
     3541 gccggcggac gggtcagcaa tccgcgtgac ggccgcggat gacgaggccc cactgcgttg
     3601 tagtaagaat tgaacagaaa gcagaatgac ggatcgaata ctcggtagtc cggcacgtgg
     3661 ggaatcagca cgaactgctc gaagaaccac gtcgtatgcg cgcggtgcca tttcgccggg
     3721 ctggcgtctg gcatcgattg gacgcactgg tcctcttcgg tcagttcggc gaccagcgcc
     3781 tccatcgcgc cacgtacgac ttcgaactgc ctggacaggt cactggcgcg ggccgtttca
     3841 gggtatagcc caatatcgtc catgtttcct ccggaggacc ctcaacgtcc ctggttgttc
     3901 cgcggtaccg tcggcgcgaa cattttctcg ttattccgtg aacgagccgc gccactttag
     3961 aaggaattgg cagaagttca aaaaccgcaa cccctgaagc agcgttcctt caggatcatg
     4021 gcaaaccggc ccatctggcg cggccacctg cgtttggcgc tggtgtcctg ccccatcgcc
     4081 ctccacaccg tcgtccgcgc gagcagcggc ctgcatttcc acctgatcaa tcccaagacc
     4141 ggccaccgga tccgcatggt gtcgcaggat gcggagaccg agcaagaggt gtcccgccag
     4201 gacctgatca agggcttcga gttcgagaag gaccgttacg tcctactgga cgatgacgac
     4261 ttcaagcagg cgcgcatcga aagctcgagc atcctgacca tcggcaagtt tgtcgatgtc
     4321 gcgacgatcg atccgatctt cttcgacacg tcctactatg tgacccctga cggcgaggcc
     4381 gggcaggacg ttttcgtggt gctgcgcgaa gcgatccaaa agtcccgcgc cgccgcgttg
     4441 tcccgcgtcg tgattgccca gcgggaacgc gcggtcgcga tcctaccttc gggcaagggg
     4501 ctggtttgcc atacgctgca tgaacccagc gatctttggg atgccggccc gctgttcgcc
     4561 gatatcggcg acatgaagcc ggaggccgat atggtggcgc tcgcgacgca gttgatcgaa
     4621 cggcagaacg gcaagttcca gcccgaggac acccaggacc gctatgaggc caagctgcgg
     4681 gacgtcatcg atgcgaagct gaagggtgaa ggcatcacgc cggaggaacc ttcggaaccc
     4741 gaccgtggca acgtcatcga cctgatggcc gcgttgaagg ccagccttgg acccgaaaaa
     4801 tcccgccgag gaagtgccgc gaaggcgccg ggagggggta ccgcgaaggc gccggcggcg
     4861 aagaaaagcc cccgcaagcg ggcgtgataa tggccgggtt ttcacgcaac cccgcggcgg
     4921 cggcctcgtt ccttgggctt gcaaccaagg agaccgcaga tggcgaatat cgccgggctt
     4981 aaaatcgctg ttctggcgac cgatggcgtg gagcaggtcg aactcaccga acccgtgaag
     5041 gcgctgaaag cgctatcggc tcaggtccag gttatcgcac cgaagggcgg ccagatccag
     5101 ggcatgaacc acgacgagaa gggcgatcaa ctgccggtcg atcaagaact cgccagcgtt
     5161 tcacccgaac agttcgacgc gttggtgctg ccgggcgggg tcacgaaccc tgacacgctg
     5221 cggacaaatc cgcaggccgt ggcgttcgtg aagcatttcg tcgtagccaa caagccgatc
     5281 gcggcgatct gccacgggcc atggacgctg atcgaggccg agggcgtgca tggccgccgg
     5341 atgacgtcgt ggccgtcgtt gcataccgac ctgaagaacg ccggggcgaa gtgggaggac
     5401 aattcggtgg tggtggacgg gaaccttgtg acctcccgca agccggacga cctgcccgat
     5461 ttctgtcggg agatggtcaa gttgttcgaa gcgggacagt cgcgcaaggc ggcttgatcc
     5521 ggacgacggg gcgaccacgg taggccgcct tcaactagac cgttttccgt tcgcctgggc
     5581 tcactgaaaa cggtcaagct cattgtttta gcgagtatct tcacccgttc agataatcca
     5641 tctgaacggg atctgcccta aggtcgtgac ggcccagcac ctcggatccg gtcgatcagc
     5701 gcgagcggat ccgatggtgc ggcgttgcct cgccaggcga catgttggtc aggtctcgag
     5761 agcaccaatt tctcggtgta aaccggttcc ctggttgtaa catcgagtaa aaccagcggc
     5821 acacctccgt tttgcgccgc agcgagcatg cggctgacat ccacttgggg atcgaagcgc
     5881 agcagagtgt agtcagcgcc catcgcgtcg tacagtgaaa gtccttcgcc caaccagata
     5941 tggggtgtcc ggcagcccgg cacggtggac ggcgtgaagt cgtagatcgt gtattcaggc
     6001 gccgccgaat cgtcgtgagc gatgatcggc gagttctcgt aaaacgagcc gaagttcagt
     6061 ccgccacagc agaactggcc gacattgatg tcataggcat cctgtcccac ccgcgcccga
     6121 gcggcatcgc cctcgggtcc gggttccttg atattgtccg gtatcccgcc gccacgttgc
     6181 cgcgccagag ccgtggaggt atccatcgcg taatgggaca cctgctcggt gatcggcatc
     6241 cgttcggcct cgtaagagtc caggatcgcc ggcgacgccc agcccttcag cacccccgcg
     6301 agcatccatg aaaggttcat ggcgtcggcg atcccggcgt tcatgccgta gccggcgttt
     6361 ggcacccaga tatgggcggc atcgccacac aggaaaaccc gccggtcgcg gaagcggtcg
     6421 gcgatcatgc gccgagcggt ccagtcctcc ttggcgagga tctcgatcgg gaacgatggg
     6481 ccgacaccta ggatcgtgcg gatgcagtgc tcgcggtcca cgctgtcgaa ggtttcgccg
     6541 gggcgcatat agttgtgcac cagccatgtc tcgcggccgt ctatcgcgaa catgttggcg
     6601 ctgcgccgcg ggttcagcga cgtgttcgcc caggcgggct tcgtcggcgt cgcccgcagc
     6661 aagtccggtg ccttgatata gctggactgg cgctgctcca gcatcggatc gccggtcagc
     6721 ctcgcgccga tcgcacggcg gaccatcgag tgggcgccgt ccgcgccgat catgtaatcg
     6781 gcggacacgg tcaggttctc gccgccgtcc agatcggcgg ccgtcgcgga gaccccgttc
     6841 tgatgctgct cgaaggcaat gagttgcatc cgattgatga tgcgaacgcc ttgcatcgat
     6901 ttcgcatgtt cgaacagcac cggttccagg tagatctggt tgatgcgatg cggtggctcc
     6961 ggcgttggcc accatgtatc cgggcctccg gtcgcggtgt agcggtccgc tcggcagggg
     7021 atcgggatgc gggacagttc gatacccgtg gtcgtggtcc gaaaggcgac gtcgtttgga
     7081 tagtcggctg gcaagccggc gttgcgcacc gcctgcgcca cgccgaggcg gcggaaaatc
     7141 tccatggtcc tggcgctgac gtggttgcac ttgacgctgg gaggctcgcc gggggcgcgg
     7201 ctttccacga ccaggaccgg aatcccacgc caggcgaggt ccatcgccat ggtcaatccg
     7261 atcggaccgc caccggcgat caacaccttt gtcgcgatgt gcctcatgtg gctgtctcct
     7321 gatccctggc ggcagtctgc ccgcggggaa aggttatcgc cagcccgtca tcggccagtt
     7381 tacctttgcc gccgcggcat cgtctggcca gatggtgtcg tagtccttgc cctgaagctg
     7441 agtcagatat gtcgcacttt caacgttctg gccggtttcg tcgaagcgaa cgccgcgata
     7501 gccgaccatc atcgcgctgt gcggcatggc ggtcgcctgc agtgcctccc gcagcttcgc
     7561 cgggtcggcc gaaccggcac ggttcagcgc gtcggccagg acgaagaacg cctgcatgca
     7621 ccggccgctg acatcgccga tctcgttccc ggtcttggcc tggtagatcg cgttgacccc
     7681 gttggacagg ctgccggcgg gccctttgct ccagacgctg cggttgacca agcccttggc
     7741 caggccgccg acagccggca ggaacgcggg gtcggaaaaa cccgcgctgt cgccgataat
     7801 catgggcggc aggtaatcca ggcttttcat cgtccgcagg aacaggatcg cgtcggaggc
     7861 gtagctgacg aacagcgcca ccgacggatt cttctccttc aactgcagca cctgggtgga
     7921 tacgtcggca ccattggcgc tgtatggcac gcgtatggac accgggatgc ccatcttctt
     7981 cgcctcggct tcgatcgctc cgccgaccga ggtgccgtag tcggtgttct cgttgacgat
     8041 cgcgatcgag
//
LOCUS       DASWQN010000112        10904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1464106, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000112 DASWQN010000000
VERSION     DASWQN010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464106"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..4178)
                     /gene="glgX"
                     /locus_tag="VGC82_01840"
     CDS             complement(15..4178)
                     /gene="glgX"
                     /locus_tag="VGC82_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY0422047.1"
                     /translation="MTGNKGKMPIVPSYRSADRGSPDTLGVTVTGDGINVAVHAPEAD
                     AVAVCLFDAADQETSRIRLPARTGPVHHGGITGVPPGTLYGLRAFGSWNPGNGHRFNP
                     AKLLLDPWATAIDRPFRLHPLLFDRDGPRPEDTAALMPKAVVETPLAAPADNRPTFDW
                     DRQVIFETHVRGFTMTHPDIPPAIRGTFAALGHPASIRHLSRLGITTIELMPSAAWVD
                     ERHLPPLNLSDYWGYNPIAFLAPDPRLAPGGWPEVRAAVDALHGAGISVILDVVLNHS
                     GESDELGPTLSLRGLDNAGYYRLAADRSLYVNDAGCGNVLAMDRPAALRLGMDALRAW
                     ALYSGMDGFRLDLAATLGRRPSGFDPQAPLLSAIEQDPILSRRVMIAEPWDIGPGGYQ
                     LGAFPARWGEWNDRYRDTARRYWRGDAGMVGELASRFAGSADIFGARPLSRSINYITS
                     HDGFPLADLVSYEAKRNYANGEDNRDGSNDNQSWNNGMEGPSTDPAVLAARTRDVRAL
                     LATLLLSRGTPMLSMGDELGRTQHGNNNAYAQDNAGSWVDWAAADQSLIDFTAAAIAL
                     RRQLAPLFDGRTLRGVPEGDQVVPDVTWRDADGNVMSEAEWNRSSNRTLIAELFAAGV
                     RAVLVFNADSKPAEICVPATVSGYDWSRVFESIGGGIGLTVAARSVTAFQEKASGSDP
                     GLAARIPATSADAPIPDRPAATADHSATFRRGRGTATVGVADAELDRLADLAGIDLIW
                     WDVEGGYHKVSPDSKQVLLAAMRLPAATQADLRDSLARLTLKPALPPALTVGRQDSIK
                     LQLGQPRPVWVTLLRDDGSIDRFHTSGENLVLPPQPIGRHRLLNEDQPEHFCHLIVAP
                     EGCYLPPPLSAGEHRFGIAAHLYALRSKDDQGVGDFTTLARFAAEAAHAGACIVGLNP
                     LHALFPHDRSRASPYHPSDRRFLDPIYIDVSGLPGGAGIASPAGAVDYRAVWDRKRAV
                     LQRAFSPGDQAIASALQRFSTFEAIADALGSSDWQNWPTELRHPDSPGVLAFAAQHRD
                     AVAFHAFLQNVADQQLSTAAASASRDGLTLGFYRDLAIGSAPDGAEAWSAQDTLMRGV
                     SVGAPPDPFSAGGQVWGLPPPDPLAMRRDGYSAFNDLLIANMRHAGALRIDHVMGLRR
                     LFVVPEGAGASDGAYVTYPMQDLLAQVALQSQRARCLVVGEDLGTVPEGMSEALTASN
                     ILSYSVLWFERRCDGKLREPAEWRRLAAACVSTHDLPTLAGWWSGADIAERRNLSLLD
                     DPGAEQARLTAKAELVALLRAENLLIADVDLAQPIPPPVAAAVHAFVSSTPSLLAFVQ
                     ADDLAGETVAVNLPGTDRERPNWRRRLDPDVSVLCRTPLARAILVALRARSASASTGS
                     ESPVMPASP"
     gene            complement(4545..4976)
                     /locus_tag="VGC82_01845"
     CDS             complement(4545..4976)
                     /locus_tag="VGC82_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 family protein"
                     /protein_id="HEY0422048.1"
                     /translation="MSNYDIDILEWSERQASLLRRRGAGELVNDADLDWSNIAEEVED
                     VGRSSLRACRSLLLQALLHDLKAQAWPLSRDVPHWRSEARVARINAADAYAPSMRQRI
                     DVQDLYAKALRAMPETIDDQPPLPVPTVCRVTLDNLLADDP"
     gene            complement(5011..6486)
                     /gene="glgA"
                     /locus_tag="VGC82_01850"
     CDS             complement(5011..6486)
                     /gene="glgA"
                     /locus_tag="VGC82_01850"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEY0422049.1"
                     /translation="MRVLSVASEIFPLVKTGGLGDVTGALPQALGAAGATATSMVPGY
                     PAVLVALTDAVAIHRFPDLFGGPATVLRGSAGSLDLIVVDAPHLYARAGNPYLGPDGA
                     EWADNGIRFAALGAAAAALATGAVPGLTFDIVHAHDWQAAMAAVYLRFHEGVRPGTVL
                     TVHNLAFQGRYRANLFPRLGLPPAAFALDGLEYHGDVSFLKGGLSSADRLTTVSPTYA
                     HEITSLDNGMGLDGLLRHRGGVLSGILNGIDDTVWNPATDPLIPSRYARGKSVPAKRV
                     ANKAVLQQRMGLDKKPDALLIGVVSRLTSQKGLDMLVDQLDDLEAAGMQLALLGSGDP
                     DLERAFVAAAEWYLGSVGCVLGYDEELAHLIQAGSDALLVPSRFEPCGLTQLYALRYG
                     AIPVVSRVGGLADSVIDANEAALAARVATGIQFWPATPDGLQWALQRLSMLWRNRPAW
                     EQVQRNGMATDVSWRGPARRYIALYRSIQEKRGLYPPEQRA"
     gene            complement(6486..7757)
                     /gene="glgC"
                     /locus_tag="VGC82_01855"
     CDS             complement(6486..7757)
                     /gene="glgC"
                     /locus_tag="VGC82_01855"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY0422050.1"
                     /translation="MVQRPGLHAPIVRDAMAYVLAGGRGSRLLELTDVRAKPAVYFGG
                     KSRIIDFALSNALNSGIRRIGVATQYKAHSLIRHLQRGWNFFRPERNEGFDILPASQR
                     SGDNWYAGTADALYQNIDIIEGYNPEYIVVLAGDHVYKMDYAVMLTQHVERGADVTVG
                     CIEVPRMEATGFGVMHVDETDRIIAFVEKPVDPPAIPGHPDTALASMGIYVFNTRFLI
                     EQLHRDEADKSSSRDFGKDIIPYLVTHGKAIAHRFTSSCVTSGAEREAYWRDVGTLDA
                     YWEANIDLTAIIPALDLYDRDWPIWTYAEITPPAKFVHEEIGRRGEAISSLVSGGCIV
                     SGSFLRQSLLFTGVHVHSYARVEHAVILPDADIGRAARLRNVIVEKGVRIPPGLVVGE
                     DAIEDARRFRRTERGICLITQPMIDRLDRQG"
     gene            complement(8242..10668)
                     /locus_tag="VGC82_01860"
     CDS             complement(8242..10668)
                     /locus_tag="VGC82_01860"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEY0422051.1"
                     /translation="MKALQTAILEKLTYQVGKRRAIATDRDWLMATAFALRDQIVDRW
                     LAGIDAAYQQGSKRIYYLSLEFLIGRLLFDNLNNLNQLDTMRTALAGLDVDLDKLRAL
                     EPDAALGNGGLGRLAACFMESMATLDVPAHGYGIRYEHGLFRQVMKNGWQQEFPEDWL
                     ALGNPWEFHRPEVNYQIGFGGAIEAVPISEAAVRHVWHPAETIEAVAYDTPSVGWRGR
                     RVNTLRLWSARSADPLRLDTFNHGDHAGALAEQVKAESIAKVLYPSDATPAGQELRLR
                     QEYFFVSASLQDLVRRHIKAFGHIGNLADKTSIQLNDTHPAIGVAEMMRILLDDHQIA
                     WEEAWRMTRATFSYTNHTLLPEALEVWPVPLMERLLPRHMQIIYLINALHLGNVRQEH
                     PNDDELLTSVSMIDEQQGRRVRMSHLAFLGSHRANGVSALHTGLMRQTVFRDLHNLFP
                     DRIVNVTNGIAFRRWLHEANPRLTRLLVEAAGSEVLDNENALTRLRPLANDRGFQKRF
                     AASRYVNKLALADLIRKELEIRVDPAALFDVHIKRIHEYKRQLLNILEAVALYNTIRA
                     RPMEDRTPRVKIFAGKAAASYHQAKLIIKLIHDVARVINSDPTVRNVLKIVFLPNYSV
                     SLAEVIIPASDVSEQISTAGMEASGTGNMKFALNGALTIGTLDGANIEILEHVGKDNM
                     FIFGLTSDQVAARRRSGSDSSDLIAASPVLAEVLDSVEAGVFSPDEPDRYRGLVNSLR
                     HHDHFLVCADFDEYYETQRRVGDLWRRPDDWWQAAILNTANVGWFSSDRSIREYADLI
                     WNVPTHSSQR"
BASE COUNT         1990 a         3474 c         3578 g         1862 t
ORIGIN      
        1 cctgcctgcc ggcactacgg cgaagccggc atgacggggg attcgctgcc agtggaagcg
       61 ctcgccgaac gagcgcgaag ggcgaccaag atggcccggg cgagcggtgt ccggcacagc
      121 accgatacgt ccggatcgag acgccgccgc cagttgggac gctccctgtc ggtgcccggc
      181 aggttcaccg ccaccgtctc gccggcaagg tcgtcggcct ggacgaaagc cagcaaggac
      241 ggcgtggatg aaacgaaagc gtgaaccgcg gcggcaaccg gcggagggat cggctgcgca
      301 agatcgacat ccgcgatcag caggttttcc gcccgcagca gcgccaccag ttcggccttc
      361 gccgtcaatc gagcttgttc ggcgcctgga tcgtccagca gagacaaatt ccgcctttcg
      421 gcgatgtccg cgccgctcca ccatccggcc aaagtgggca gatcatgggt cgacacgcag
      481 gcggcggcca ggcggcgcca ttccgccggc tcgcgaagct ttccgtcaca gcggcgttca
      541 aaccacagta cgctgtagga cagtatgttg gacgcggtca gggcttccga catgccttcc
      601 ggaactgttc ccaggtcctc gcccaccacg aggcaccgtg cccgctggct ctgcagcgcg
      661 acttgcgcca gcagatcctg catcgggtat gtgacatagg caccatcgct ggcgccggcg
      721 ccctcgggga cgacgaacag gcggcgcaga cccatgacgt gatcgatccg cagagcgcct
      781 gcgtgacgca tgttggcgat cagcaggtca ttgaaggccg agtagccgtc acgccgcatg
      841 gcgagcgggt ccggcggcgg caggccccag acctggccgc cggcggaaaa cggatccggt
      901 ggcgccccga ccgacacacc gcgcatcaat gtgtcctggg ccgaccaggc ctcggctccg
      961 tcgggcgccg aaccgatggc gaggtcgcgg taaaagccta gtgtcaggcc gtcccgactg
     1021 gcggatgcgg cagcggtgga gagttgctgg tctgccacgt tttgcaggaa ggcgtggaac
     1081 gcgacggcgt cgcgatgttg ggcggcgaag gccaaaacgc cggggctgtc gggatgacgc
     1141 agttcggtgg gccagttctg ccagtcggac gatcccagcg cgtccgcgat cgcctcgaaa
     1201 gtcgaaaacc gctgcagcgc ggaggcaatc gcttggtcgc cggggctgaa agcgcgctgt
     1261 aataccgccc gcttgcggtc ccaaaccgcc cggtaatcga ctgcacccgc cggggaggcg
     1321 atgccggcgc caccgggcag gccggagaca tcgatataaa tcggatcgag gaaacgccgg
     1381 tcggacgggt gatacgggct ggctcggctc cggtcgtgcg gaaaaagcgc gtgcagcggg
     1441 ttcagaccga cgatacaggc gccggcatgg gcggcctccg cggcgaagcg ggcaagcgtg
     1501 gtgaaatcac cgaccccctg atcgtccttg gaacgcaacg cataaagatg agcggcgatg
     1561 ccgaagcggt gttcccccgc cgaaagcggc ggtggcaggt agcagccctc tggcgccacg
     1621 atcaggtgac agaagtgttc gggctggtct tcattcaaca atcgatgccg gccgatgggc
     1681 tgcggcggca acacgagatt ctcgccggag gtgtggaagc ggtcgatgct gccgtcatcg
     1741 cgcagcagcg tgacccagac ggggcgaggc tggccaagct ggagcttgat gctgtcctgg
     1801 cggccgaccg tcagcgccgg aggcaaggcg ggcttcaagg tcaaccgtgc caggctgtcg
     1861 cgaaggtccg cttgcgtggc ggccggcaag cgcattgcag ccaacagcac ctgcttgctg
     1921 tcggggctga ccttgtgata gccgccctca acgtcccacc agatcaggtc gatgcccgca
     1981 aggtcagcta accggtcgag ctccgcatca gccacgccga ccgtcgcggt gccacggccg
     2041 cggcgaaaag ttgcgctatg atcggcggtc gccgcgggcc tgtccggtat cggcgcatca
     2101 gcgctggtag ccggaatcct agccgccaag ccgggatctg acccgctggc tttttcctga
     2161 aacgccgtca ccgaccgagc tgcgacggtt aaaccgatac cgccgccaat gctctcgaag
     2221 acgcgcgacc aatcataccc ggacacagtc gccggcacgc aaatttccgc cggtttggaa
     2281 tccgcgttga acaccagcac ggcccgcacg ccagccgcga acaactcggc gatcagcgtg
     2341 cggttgctgc tccggttcca ctccgcctcg gacatcacgt tgccgtccgc gtcgcgccag
     2401 gtcacgtccg gcacgacctg atcgccctca ggcacgccgc gcaaggtccg gccgtcgaat
     2461 aacggtgcca gttgccggcg cagcgcgatc gcggcggcgg tgaagtcgat cagcgactga
     2521 tccgcggcgg cccaatccac ccaggagccg gcattgtcct gcgcgtaggc gttgttgttg
     2581 ccgtgctgtg tccgcccaag ctcgtcgccc atcgacagca tcggcgtgcc gcgcgacagc
     2641 agcagcgtcg ccagcagcgc acgaacatcc cgggtgcgtg cggcaaggac ggcggggtcc
     2701 gttgacggac cctccatccc gttgttccag gactgattat cgttcgatcc gtcgcggtta
     2761 tcctcgccat tggcgtagtt tcgcttggcc tcgtaggaaa ccaggtcggc aagcgggaag
     2821 ccatcatgcg acgtaatgta gttgatgctc cgcgagagcg gccgggcacc gaagatgtcg
     2881 gccgacccgg caaagcggga ggcgagttcg ccgaccatgc cggcatcacc gcgccagtag
     2941 cgccgcgcgg tgtcgcggta gcggtcgttc cattcgcccc atcgggcggg aaatgcgcca
     3001 agctggtagc ctccgggtcc gatgtcccag ggttcggcga tcatcacgcg gcgggacagg
     3061 atcgggtcct gttcgatcgc cgacagcaac ggcgcctgcg gatcgaagcc ggatggccgc
     3121 cggcccagcg tggcggcaag atccagccgg aacccgtcca tcccgctgta aagcgcccag
     3181 gcccgcagcg cgtccatgcc caggcgcagc gcggccgggc gatccatcgc cagcacattg
     3241 ccgcagccgg cgtcgttcac gtaaagcgag cggtcggcgg ccagccggta gtatccggcg
     3301 ttgtccagcc cgcgcagcga caaagtcggg ccaagctcat cgctctcgcc ggaatggttc
     3361 agtaccacgt ccagaatgac gctgatgccg gcgccgtgca gcgcatcgac ggcggcgcgc
     3421 acctcgggcc atccgccggg agccagccgg ggatcggggg cgaggaaggc aatcgggttg
     3481 tagccccagt aatccgacag attgagtggt ggcaagtgcc gttcgtccac ccaagcagcg
     3541 ctgggcatca gctcgatcgt cgtgatgccc aggcgcgaga gatgccggat gctggcggga
     3601 tggccgagtg cggcgaaggt tccccggatc gccggtggga tgtccgggtg cgtcatggtg
     3661 aagccacgga cgtgagtttc gaagatgacc tggcggtccc aatcgaaagt agggcggttg
     3721 tcggccggtg cggccagtgg ggtttccacg acagccttcg gcatcagcgc ggcggtgtcc
     3781 tcggggcgtg gcccgtcgcg gtcgaacagc agcggatgca gccggaatgg ccggtcgatc
     3841 gcggtcgccc aaggatcgag cagcaacttc gccggattga agcggtgacc gttgccggga
     3901 ttccaggagc cgaaggctcg caggccgtag agcgtgccgg gcgggacgcc ggtaatgccg
     3961 ccgtggtgaa cggggccggt ccgggcggga aggcggatgc gggacgtttc ctggtcggct
     4021 gcgtcgaaca ggcagaccgc gacggcgtcc gcctccggcg catgaaccgc aacgttgatc
     4081 ccgtcgccgg tgacggtgac ccccagcgta tccggcgatc cccggtcggc tgaacgatat
     4141 gaaggaacga ttggcatttt gcccttgtta cctgtcatgc tcgcctcccg ttccggcgcg
     4201 acccataggt ttccaaggcg tatgtcgagc attcagaggc atctgtgtcc gatccgagga
     4261 acatgatcca attgggagtc gcggtatccg gcaggggcgg tgcgaatcag gagtggtctg
     4321 ctgacgacgg catatcggca agatccgcaa gtactacggc gccaatcgac cgtgcatgcg
     4381 gtgctgttca ttcacgtcaa tgaccggtgc aagcttattg cgatcatgct gtgttacgcc
     4441 gggccgaaaa taacgaccgg cggaaggccg gacgatcctg gatcgcttgg acgacgaagc
     4501 cgtgtcgggc aaataagcaa acgcttcgcc tgctgcaggt tgaatcaggg atcgtcagcc
     4561 aacaggttat ccagggtaac gcggcagacg gtcggtaccg gcaagggcgg ttgatcgtca
     4621 atcgtctccg gcatcgcacg caacgctttg gcgtatagat cctgcacatc gatccgttgt
     4681 cgcatcgatg gcgcataagc gtcagcagca ttgatccggg cgacccgggc ctccgaacgc
     4741 cagtgcggca catcgcgtga cagcggccaa gcctgggcct taaggtcatg cagtaaggcc
     4801 tggagcagca aggatcgaca cgcgcgcagg ctgctgcgtc ccacgtcctc tacctcctcg
     4861 gcgatgttgg accagtccag atccgcgtcg ttgaccagtt cgccggcgcc gcggcggcgc
     4921 agcagcgagg cctggcgctc cgaccattcc aggatatcga tgtcatagtt gctcatcgga
     4981 tgttctccat gagtgcggag gcggtttgaa tcaagcacgc tgctcaggag gatataagcc
     5041 gcgcttctcc tgaatcgacc ggtacagcgc gatatagcgt ctcgcggggc cgcgccagga
     5101 gacgtcggtt gccatgccgt tgcgctgcac ctgttcccac gctggccgat tgcgccacaa
     5161 catcgacaat cgctgcaatg cccactgcag tccgtccggt gtcgcgggcc agaactgaat
     5221 accagtcgcc actctcgcgg ccagggcggc ctcattggcg tcaatcaccg agtccgctag
     5281 gccgccgaca cgcgaaacca ccgggatggc gccgtagcgc aaggcataga gttgggtcag
     5341 tccgcagggc tcgaaacggg acggcaccag caacgcgtcg ctgccggcct ggatcaggtg
     5401 ggccagttct tcatcataac cgaggacgca tccgacggag cccaggtacc attccgcggc
     5461 agcgacgaag gcgcgctcca ggtcggggtc tcccgatccc agtaaggcaa gctgcatgcc
     5521 ggcggcttca agatcgtcaa gctggtccac cagcatgtcc agtcccttct gcgaggtcag
     5581 gcggctaacg acgccgatca gcagcgcgtc cggctttttg tcgagaccca tgcgctgctg
     5641 cagcacggcc ttgttggcca ctcgtttggc cggcacggat ttgccgcggg catagcgcga
     5701 tgggatcagg gggtcggtcg ccgggttcca gacggtgtcg tcgatgccgt tcaggatgcc
     5761 cgatagcacg ccgccgcggt ggcgcagcaa gccgtccagt cccattccgt tgtccaaact
     5821 ggtgatctcg tgcgcatagg tgggcgagac cgtggtcagg cgatcggccg aggacaggcc
     5881 gcccttcagg aagctgacgt cgccatggta ttccaagcca tccagggcga atgccgccgg
     5941 cggaagcccc agccggggaa acaggttggc gcgataacgg ccttggaaag ccagattgtg
     6001 gacggtcagc acggtgccgg gccgcacacc ctcgtggaac cgcagataca ccgcggccat
     6061 cgccgcctgc cagtcatggg cgtgcacgat atcgaatgtc agcccgggga cggcgccggt
     6121 cgccaatgcc gccgccgccg caccgagtgc cgcgaaacga attccgttgt cagcccattc
     6181 cgcgccgtcg gggccgagat acgggttgcc ggcacgggca taaaggtgcg gcgcatcgac
     6241 cacgatcaga tccagtgatc cggcgctgcc tcgcagcacc gtggccggac ctccgaacag
     6301 gtcggggaag cgatgaatcg cgacagcgtc ggtcaaagcg accagaacgg caggatagcc
     6361 cggcaccatc gaggtggctg tggcgccggc ggcaccgagc gcctggggca gggcgccggt
     6421 gacatcgccc aacccgccgg tcttgaccag cgggaagatc tccgacgcga ccgagagaac
     6481 gcggatcaac cttggcgatc caggcggtcg atcatcggct gcgtgatcag gcaaattccc
     6541 cgctcggtgc ggcggaaccg gcgagcgtcc tcgatcgcgt cctcaccgac gaccaggccc
     6601 ggtggaatcc tgacgccctt ctcgacgatg acgttacgta accgcgcagc ccggccgata
     6661 tctgcgtccg gcagaatgac cgcgtgctcg acgcgggcat aagagtggac atgcacgccg
     6721 gtaaacagca gcgactgccg caggaacgaa cccgacacga tgcatccgcc cgacaccagc
     6781 gaggaaatcg cctcaccccg ccggccgatt tcttcgtgga cgaacttggc cggcggggtg
     6841 atctcggcat aggtccagat cggccagtcg cggtcgtaaa gatccagcgc agggatgatc
     6901 gcggtcagat cgatattggc ctcccagtac gcatccaggg tgccgacatc gcgccagtag
     6961 gcctctcgtt cggcgcccga ggtgacgcag gaactggtga aacgatgggc gatggctttg
     7021 ccgtgcgtca ccagatacgg aatgatgtcc ttgccgaagt cccgactcga ggatttgtcc
     7081 gcttcgtcac ggtggagttg ctcgatcagg aacctcgtgt tgaagacgta gatgcccatg
     7141 ctggccaggg cggtgtcggg atggccggga atggcgggtg gatcaacagg cttctcgacg
     7201 aaggcgatga tgcgatccgt ctcgtccaca tgcatcacgc cgaagccggt ggcctccatc
     7261 cgcggcacct cgatgcagcc gaccgtaacg tccgctccgc gttcgacgtg ctgtgtcagc
     7321 atcaccgcgt agtccatttt gtagacatgg tccccggcca ggacgacgat gtattcgggg
     7381 ttgtagccct cgatgatatc gatgttctgg tacagcgcgt cggcggtgcc ggcataccag
     7441 ttatcgccgg atcgctgcga cgcgggaagg atgtcgaagc cttcgttccg ttccggccga
     7501 aagaagttcc agccgcgttg caggtgccgg atcaggctgt gcgccttgta ctgcgtggcg
     7561 acgccaattc gccggatgcc ggaattgagc gcattggaca acgcgaagtc gatgatccgg
     7621 cttttgccgc cgaaatagac cgccggcttg gcgcgcacat ccgtcagttc cagcaaccgc
     7681 gaccctcggc cgccagcaag gacgtacgcc atggcgtcgc ggacgatcgg ggcatgcaat
     7741 ccgggccgct gcaccatcaa gccgtctcct gtctgcggtt cttgtcgttg ccctaaggta
     7801 agcgtgaacc cggaggcatg ccaggatgtg gcggatcggt atctgtcacg gagcggtgtg
     7861 gcgccggcca gcgcaggtga gtgccgtggc gcaacatttc ccgtggggcg ccttcaagtt
     7921 ggctgggaaa gttgcgagcc ggtttgccgc ctgacgtcca tgtgtcatga ccgtgcttgg
     7981 cgcggccacc cacgtttttg tctgcttgcg gaacgtcgcc ggggtggcgg cacaagggca
     8041 cgcaggacag gaccgtaatg atcgtgccag agcggcccga ccgaacaatc cacctgcgcc
     8101 aaacctcagc ggaaaatatg cttcggcatc aaaccgcgct acatagcgtg gacccggcgc
     8161 tgaactcacg gcctgccgct gccacagcca acgagaagcc gtcgatcggc ctccgtcatg
     8221 cggacggacc gcacgccacc gtcagcgctg gctactgtgc gtgggcacgt tccagatcag
     8281 gtcggcgtat tcgcggatcg agcggtcgga agaaaaccag ccgacattcg cggtgttcag
     8341 gatggcggcc tgccaccagt cgtccggccg ccgccacagg tcgccgaccc ggcgctgggt
     8401 ttcgtaatac tcgtcgaaat ccgcgcaaac caggaagtgg tcgtggtggc gcagcgaatt
     8461 gaccaggcca cgataccggt ccggctcgtc gggggaaaac accccggcct ccacgctgtc
     8521 cagcacttcc gccagaaccg gggacgcggc gatcaggtcg ctggaatcgc taccggaacg
     8581 tcgccgcgcc gcgacctgat cggaggtgag gccaaagatg aacatgttgt ccttgccgac
     8641 atgttccagg atctcgatgt tggcgccatc gagcgtgccg atcgtcagtg caccgttcag
     8701 agcgaacttc atgttgccgg tgccggacgc ctccatcccg gcggtggaga tctgctccga
     8761 cacgtcggac gccggaatga tgacttccgc caggctgacg gagtagttcg gcaggaacac
     8821 gatcttcagg acgttgcgga cggtcggatc gctgttgatc accctggcga cgtcgtggat
     8881 cagcttgatg atcagcttcg cctgatgata gctggccgcc gccttgcctg cgaagatctt
     8941 gaccctgggc gtgcggtctt ccatcggccg cgcccggatc gtgttgtaca gcgcgacggc
     9001 ttccaggata ttgagaagtt gccgcttgta ctcgtggatg cgcttgatgt ggacatcgaa
     9061 tagcgcggcc ggatcgacgc ggatttccag ttccttgcgg atcaggtccg ccagcgcgag
     9121 cttgttgacg tagcggctgg cagcgaagcg cttctggaaa ccgcggtcgt tcgccaacgg
     9181 gcgcagtctg gtcagcgcgt tctcgttgtc caacacctcg gagcctgcgg cctcgaccag
     9241 cagccgggtc agcctgggat tggcttcgtg cagccagcgc ctgaaggcga tgccgttggt
     9301 gacgttgacg atgcgatcgg gaaacaggtt gtgcaggtcg cggaacaccg tctgccgcat
     9361 caggccggta tgcagcgccg acacgccgtt ggcccggtgc gaccccagga acgcgaggtg
     9421 gctcatgcgc acgcgccggc cctgttgctc gtcgatcatc gacacgctgg tcagcaactc
     9481 gtcgtcgttc ggatgttcct ggcggacgtt accaagatgc agcgcgttga tcaggtagat
     9541 gatctgcata tggcgcggca gcaaccgctc catcagcggc accggccaaa cctccagcgc
     9601 ctccggcagc agggtatggt tggtgtagct gaaggtggcg cgcgtcatgc gccaggcttc
     9661 ctcccaggca atctgatggt cgtcgagcag gatgcgcatc atctcggcga cgccgatcgc
     9721 cggatgcgtg tcgttcaact ggatagacgt cttatcggct aggttgccga tatggccgaa
     9781 cgccttgatg tggcggcgca cgagatcctg cagcgatgcc gagacgaaga aatactcctg
     9841 ccgcagcctc aattcctgcc cggccggcgt tgcgtcggac ggatacagca ccttggcgat
     9901 tgattcggct ttgacctgtt ccgccagggc accggcatga tcgccatgat tgaaggtgtc
     9961 cagccgtaac ggatcggccg accgcgcgga ccacaaccgc aacgtgttga cccgccgccc
    10021 gcgccagccg accgagggcg tgtcataggc cacggcctcg attgtttccg ccgggtgcca
    10081 gacgtgccgc accgcggctt ccgaaatcgg caccgcctcg atcgcgccgc cgaagccgat
    10141 ctggtagttc acctcgggcc gatgaaactc ccacgggttt ccaagcgcca gccagtcttc
    10201 gggaaattcc tgctgccagc cgttcttcat cacctggcga aacagcccgt gctcgtagcg
    10261 gatcccatag ccgtgggcgg gaacgtcgag cgtcgccatg ctctccatga agcacgccgc
    10321 aagccggccg agacccccgt tgcccaacgc ggcatcgggc tctagcgctc gcagcttgtc
    10381 cagatcgacg tccagaccgg ccaacgccgt ccgcatcgta tccaactggt tcaggttgtt
    10441 caggttgtcg aacaacaggc ggccgatcaa aaactccagc gacaggtagt agatccgttt
    10501 cgatccctgc tggtaagccg cgtcgatccc agccaaccat cgatcgacga tctggtcgcg
    10561 cagtgcgaag gccgtcgcca tcagccaatc gcggtcggtg gcaatcgccc ggcgctttcc
    10621 cacctggtac gtgagtttct ccaggatcgc ggtctgtaac gctttcacag ccgcgtcatt
    10681 gcttgcggta tcatgggcgt ctgcctcatc cgcgaacccg gccggcacgt tcgattcaag
    10741 ctccacgctg cttcaccttt tcgcgatgta ggccataggc caacgaaagc atgtttaacg
    10801 cctccgcttc aaccatttgc tcccgatgtt tcccatttca ccgaaatgag gcaaaaattt
    10861 caccgagtct cgaaccgcgg ggcgaaaaaa taggcagtta tggc
//
LOCUS       DASWQN010000113         1743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2124296, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000113 DASWQN010000000
VERSION     DASWQN010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124296"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1181)
                     /locus_tag="VGC82_01865"
     CDS             complement(225..1181)
                     /locus_tag="VGC82_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0422052.1"
                     /translation="MYLNIFAQGTAPLDMTGSEEFRSLLASAADLGIPHAPDVPYRSC
                     NIVLRQMRFHFLEWGRADAPVIVLLHGGHQSAHSWDLVSLHLAARYRVLALDQRGHGD
                     SEWARDVNYSNNEMSLDAEAFMAAMGLQKPIIIGHSMGGRNTMLLLRRNPAVARAAVI
                     VDVGPELSEKGRTAIAGFVQGNQEFDDLEHFVRNVRQYDPYRPREHIERTVKYNMLER
                     ADGKYVSKCDSNPRRLGIVRGSGPLENITLQDAAMFDLPVLLVRGANSNILAPDAAER
                     FAAALPRGRMVTVPDCGHNVHGQNTKGFIGALGEFLNQVGSL"
     gene            1300..>1743
                     /locus_tag="VGC82_01870"
     CDS             1300..>1743
                     /locus_tag="VGC82_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0422053.1"
                     /translation="MTQSALDRARLYQDELSAIRRDIHAHPELGLEEYRTADLVAQKL
                     AEWGIEVHRGVGKTGVVGVLRNGNGQVAVGLRADMDALPILEATGLPHASQNPGRMHA
                     CGHDGHTTMLLGAAKYLAETRNFSGTVNFIFQPAEEGVGGAQEMLK"
BASE COUNT          338 a          578 c          514 g          313 t
ORIGIN      
        1 ctggcaatgg ctaattggag cgttttcacg ctgactgaaa aaggtgccag ccagttcgcc
       61 gcctggcgcc tatgtgtcat ggccgtgctt ggcgcggcca cccacgaatt tcttgcaagc
      121 cgcgagagaa gccctgtttc cagtcaccgt gaaaccgccc tgacccggtg cgccgcaggc
      181 tgcgcggccg agaaacggga cgctgcgcaa tcactacatc atcgtcaaag cgaaccaacc
      241 tggttcaaaa attcccccaa ggcgccgata aatcccttcg tattctgccc atgaacgttg
      301 tgtccgcaat ccggcaccgt gaccatccgt ccgcgcggca gtgccgcggc aaaccgttcg
      361 gctgcatccg gcgccaggat gttggagttc gcaccgcgta ccagcagcac cggcagatcg
      421 aacattgcgg cgtcctgcag cgtgatgttt tccagcggcc cggagccgcg aacgatcccc
      481 agccgtcgag gattggaatc gcatttgctg acgtatttgc catcagcacg ctccagcatg
      541 ttgtacttga ccgtccgctc gatgtgctcg cgcgggcgat acggatcgta ttgacgcacg
      601 ttgcgcacaa aatgctccag gtcgtcgaac tcctgattgc cctgcacaaa ccccgcaatc
      661 gccgttcggc ctttctccga cagttctggc ccgacatcga cgatgactgc cgcccgtgcc
      721 acggctggat tgcgccgcag cagaagcatc gtgttgcgcc cgcccatcga gtgaccgatg
      781 atgatcggct tttgcaagcc catggcggcc atgaacgctt cggcatccaa cgacatctca
      841 ttgttggaat agttcacatc ccgagcccac tcgctgtcgc cgtggccgcg ctggtccagt
      901 gccaggacgc ggtaacgcgc cgccaaatgc aggcttacca ggtcccagga atgcgccgac
      961 tgatggccgc catgcagcag cacgatcacc ggcgcgtccg cccgccccca ttccaggaag
     1021 tggaaccgca tctggcgcag cacgatgttg cacgaacggt aggggacgtc cggcgcatgc
     1081 ggaattccca aatcggcggc actggccagc aggctgcgga actcctccga accggtcatg
     1141 tccagcggcg ctgtcccctg ggcgaagata ttcagataca tcgtgccgtc gctcccgtgg
     1201 cttccggcaa caacaccctg accggtctgg catggcaaga cccgcgtggt atgctggttg
     1261 cttgaaccgt tcctttgaac ccttggccag gatccgccga tgacccaatc cgcgctcgac
     1321 cgtgcccgcc tttatcagga cgaattatcc gcgattcgcc gcgatatcca cgcccatccc
     1381 gaattgggcc tggaggaata ccgaacggcc gatttggtcg cccaaaaact ggcggaatgg
     1441 gggatcgagg tgcatcgcgg cgtcggcaag accggcgtgg tgggcgtgct gcgcaacggc
     1501 aacggccagg tcgcggtcgg gctacgggca gacatggacg cgctgccgat cctggaggcg
     1561 accggcttgc cgcatgccag ccaaaacccc ggccgcatgc acgcctgcgg ccatgacggc
     1621 cacaccacga tgctgctggg tgccgcgaaa tacctggctg aaacgcgcaa cttcagcggc
     1681 accgtcaact ttatcttcca gccggccgag gaaggcgtcg gcggcgcgca ggaaatgctg
     1741 aag
//
LOCUS       DASWQN010000114         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1332536, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000114 DASWQN010000000
VERSION     DASWQN010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332536"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VGC82_01875"
     CDS             <1..499
                     /locus_tag="VGC82_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422054.1"
                     /translation="GVSLATAMTIFGFLAAVNAMKRSAAIGQTDAGIAFAVVPLSGIL
                     FFAVVFTLAVAATGRPETHKRLMLLAGISILDAAVARWFLTFLAPPGPPGPPPVEVTI
                     VPALIAYLLLVAAIIFDWRTRGRPHPVYIYGGAALVAVKLLNWPISVTPAWHSFAGGI
                     LALAQ"
     gene            845..>1531
                     /locus_tag="VGC82_01880"
     CDS             845..>1531
                     /locus_tag="VGC82_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY0422055.1"
                     /translation="MPNDDVIVVSDEALLKAESFVEAEEGAANRLMGWAGRISTAIAV
                     VMSLFHLYAAYAIVPTQELRYTHVAFTLVLSFLLFPLATRFRNRVRWWDVVPGIFAVA
                     TIVYALWGGDDFTDRATSPDRWDVIVGIVFIVLLLEATRRTTGLIMPVVSLLFIAYAM
                     LGPHLPAPWTHRGYDLPRLVGHLFITLEGIFGVAVDVSATLIILFTIYGAFLQQSGAG
                     KFFIDFSLALM"
BASE COUNT          243 a          496 c          446 g          346 t
ORIGIN      
        1 cggtgtatcg ctggcgaccg cgatgacgat cttcggtttc ctggcggcgg tcaatgcgat
       61 gaagcgttcc gcggcgatcg gacaaaccga cgcaggaatt gcgtttgctg tcgttccgct
      121 cagcggcatt ctgttcttcg cggtggtgtt tacactcgcg gttgccgcca ccggtcggcc
      181 cgagacgcac aagagattga tgctgctggc cggcatctcg attctggatg cggcggtggc
      241 ccgctggttt ctcacgttcc tggcgccgcc cggaccaccg ggcccgcccc cggtcgaagt
      301 caccatcgtc ccggccttga tcgcctatct gctgctcgta gccgcgatca ttttcgactg
      361 gcgcacgcgc ggtcgtccgc atcctgtgta catctacggc ggcgctgccc tcgtggccgt
      421 gaagctgctg aactggccca tcagtgtcac gcctgcctgg cactccttcg caggtggaat
      481 attggcgctc gctcagtagc gcgaggcagg gttatggctc cctgctttcg taagggacga
      541 cgagaaagac caactcacct tgagcgtaac cccgccatcg accaccagtt ctatcccggt
      601 gacgtatttt ggattcgtct gaagccagaa accgcattgg cgacgtccca ggctccccca
      661 tgttcggcaa tctccatggc gcaccgtgcc aataatccca aagtctctga tccgatgccc
      721 ttgccaccgt ttgcccaaac cggcatcaat gcgcctgcaa ccaacgccac ggccagttag
      781 ccaggccatg taagagcgtt ccggggggag catggccgct gacaatccat cgccaggcaa
      841 ttcattgccc aatgacgacg ttatcgtcgt ttccgacgaa gcgttgctca aggcggaatc
      901 gttcgtcgaa gccgaagagg gcgcggccaa ccgcctgatg ggctgggcgg ggcgaatctc
      961 gaccgccatt gccgtcgtga tgagcctgtt ccatctctac gcggcctacg caatcgttcc
     1021 gacgcaggaa cttcgctaca cccacgttgc attcacgctg gtgctcagct tcctgttgtt
     1081 tccgctggcg acgcgttttc gcaaccgtgt gcgctggtgg gacgtcgtgc ccggcatttt
     1141 tgcagttgcg actatcgtct atgccttgtg gggcggtgac gatttcaccg accgcgccac
     1201 ctcgcccgac cgctgggacg tcatcgtcgg catcgtgttc atcgtgttgt tgctggaggc
     1261 gacacggcgc accaccggcc tgatcatgcc cgtggtgtcg ctattgttca ttgcctatgc
     1321 gatgctcggg ccgcatctgc cggcgccgtg gacccatcgc ggctacgacc tgccccgtct
     1381 ggtcggtcac ctcttcatca cgctggaagg cattttcggc gtcgcggtcg atgtgtcggc
     1441 gaccctgatc atcctgttca cgatttacgg cgcgttcctg cagcaatccg gcgcgggcaa
     1501 attcttcatc gatttctcgc tggcgctgat g
//
LOCUS       DASWQN010000115         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_277044, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000115 DASWQN010000000
VERSION     DASWQN010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277044"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /gene="tcuA"
                     /locus_tag="VGC82_01885"
     CDS             complement(<1..1226)
                     /gene="tcuA"
                     /locus_tag="VGC82_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEY0422056.1"
                     /translation="MADRTKTYDVLIAGGGNAALCAAITARQAGASVLLVESAPKAFR
                     GGNSRHTRNLRAMHDLPNEVLTDAYKEDEYWDDLLRVTGGNTDEALARMTIRASSGVF
                     GWMKSAGVRFQPSLTGTLNLGRTNAFFLGGGKALVNAYYLTAAKLGVEILYDSEVVSL
                     NMDDGSVRSIDITYKGFPETVHARCVVASSGGFQANLDWMREYWGDAVDNFVIRGTPY
                     NRGRVLKNLLGQGVASIGDPTQCHAVALDARAPKFDGGITTRLDSVPYSIVVNRNGDR
                     FYDEGEDVWPKRYAIWGRLIAQQPGQIAYSICDAKAFPLFMPSVFPAIKADTLEELAI
                     KMKLDPTKVRETIDGFNKAIKPGQVFDNLKLDGNATDGLTPNKTNWARSLDKPPFYGY
                     PLRPGITFTYLGVRVN"
     gene            1322..1855
                     /locus_tag="VGC82_01890"
     CDS             1322..1855
                     /locus_tag="VGC82_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HEY0422057.1"
                     /translation="MAEATEERAWYEIVRDVLKRHDVSLVTFVPDNVLRPLISAIEAD
                     PFFTAFACTREEEALGINAGAWMAGVKGILLMQTSGFATLPNALASLSVPFQIPTLMM
                     VSERGTLGEFNIGQSLVARTMRPVLDALAIENHTMTRLDETEFIVDRSLTQAFATMAP
                     VCFILSPLLTGGKVFSK"
     gene            1869..>2269
                     /locus_tag="VGC82_01895"
     CDS             1869..>2269
                     /locus_tag="VGC82_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0422058.1"
                     /translation="MADLSNQPVGRDQAKVMNRYNISKRMIGLLKQDEAVIGGIGYTN
                     FDLWAASASASGRRQQNFYMLGSMGLAVPIALGVAIAQPHRKVFALEGDGSVLMQLGS
                     LSTVAARRQKNLCIVIMDNGAYQITGGQRTMT"
BASE COUNT          403 a          653 c          756 g          457 t
ORIGIN      
        1 tcgttcacgc ggacgcccag gtaggtaaag gtgatgccgg ggcggagcgg gtaaccgtag
       61 aatggcggct tgtccaggga gcgagcccag ttggtcttgt tcggggtcag cccgtcggtg
      121 gcgttgccat ccagtttcag gttgtcgaac acctggccgg gcttgatggc cttgttgaag
      181 ccgtcgatgg tttcgcgaac cttcgttggg tccagcttca ttttgatggc gagttcttcc
      241 agtgtatccg ccttgatggc ggggaagacc gatggcatga acagcggaaa ggctttcgcg
      301 tcgcagatcg agtaggcgat ctggccaggt tgttgcgcaa tcaggcggcc ccagatggcg
      361 tagcgcttcg gccagacatc ctcgccttcg tcgtagaagc ggtcgccgtt gcggttgacc
      421 acgatgctgt agggcacgct gtccagacgc gtggtgatcc cgccgtcgaa tttcggcgcg
      481 cgggcgtcga gggctacggc gtggcactgt gtgggatcac cgatcgatgc cacaccctgg
      541 ccgagcaggt ttttcagcac gcggccgcgg ttatacggcg tgccgcggat gacaaagttg
      601 tccaccgcgt cgccccagta ttcgcgcatc cagtccaggt tcgcctggaa gccgccggag
      661 gatgccacca cgcaacgcgc atgcaccgtt tcgggaaaac ccttgtaggt gatgtcgatc
      721 gatcgcaccg aaccatcgtc catgttcagc gacacgactt cactgtcgta caggatctcg
      781 acaccgagtt tcgccgcggt caggtagtag gcgttcacca gcgccttgcc gccacccagg
      841 aagaaggcgt tggtgcggcc gaggttcagg gttccggtca gcgacggttg aaacctcaca
      901 ccggcgctct tcatccagcc aaacacgccg ctggaggccc tgatcgtcat ccgagcaagg
      961 gcttcgtcgg tgttgccgcc ggtgacgcgc agcaggtcgt cccagtattc atcttccttg
     1021 taggcgtcgg taagcacctc gttcggcaag tcgtgcatgg cgcgaagatt gcgcgtgtgg
     1081 cggctgttgc cgcctcggaa cgccttgggg gcgctttcga ccagcagcac gctggctccc
     1141 gcctgacgcg cggtgatcgc ggcgcacaag gcggcattgc cgccgcccgc aatcagcacg
     1201 tcgtaggttt tcgtccgatc agccatccac tccctccgcc gccggttctt tcctacgaac
     1261 ggttgcggga tcgtgcaagg cgtgcgaatc cggcgatcgg gttcgaatga tgggggacga
     1321 gatggcggaa gccaccgaag agcgcgcttg gtacgagatc gttcgcgatg tgctgaagcg
     1381 ccacgacgtc agcctggtta ccttcgtgcc ggacaacgtg ctgcgtccgc tgatatcggc
     1441 gatcgaggcc gatcccttct tcaccgcttt cgcatgcacg cgggaggaag aggcgctggg
     1501 catcaatgcc ggtgcctgga tggccggggt aaagggaatt ctgctgatgc agacctccgg
     1561 cttcgcgacg ttgccgaatg cgctcgcatc gctgtcggtg ccgtttcaga tcccgacgct
     1621 gatgatggtg tcggaacgcg gaacgctcgg cgaattcaac atcggccagt cgctggtggc
     1681 gcggaccatg cgcccggtgc tggatgcgct ggcaattgaa aatcacacga tgacgcggct
     1741 ggacgagacg gagttcatcg tggaccgcag cctgacacag gcgttcgcaa caatggcgcc
     1801 cgtttgcttc atcctgtcgc cattgctgac cggcggcaaa gtgttttcga aataggcggg
     1861 ggtgcttcat ggccgatttg tcgaatcaac cggtaggccg ggaccaggcc aaggtgatga
     1921 accgctacaa catcagcaag cggatgatcg ggctgctgaa acaggacgag gccgtcatcg
     1981 gcggcatcgg ctacacgaat ttcgatttgt gggcggcttc cgcctcggcg tcgggacggc
     2041 ggcagcagaa cttctatatg ctgggcagca tgggattggc ggtgccgatc gcgttggggg
     2101 tggcgattgc tcagccgcat cgaaaggtat ttgccctgga aggggacggg tcggtgctga
     2161 tgcagcttgg atcgctgtcc acggtggcag cgcgccgtca gaagaacctg tgcatcgtga
     2221 tcatggataa tggcgcatac cagatcaccg gtggacagcg gacgatgac
//
LOCUS       DASWQN010000116         7936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1332567, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000116 DASWQN010000000
VERSION     DASWQN010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332567"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(381..764)
                     /locus_tag="VGC82_01900"
     CDS             complement(381..764)
                     /locus_tag="VGC82_01900"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEY0422059.1"
                     /translation="MKATLCAAALIVGAALVPASAQPPHSNASSPSPEMSTHGTGRSP
                     SSDAFRAADERMMHDMAAPMTGDADRDFVAGMLPHHRGAIEMAKVELQYGKDKELRRL
                     ATAIVQAQEKEIAQMKAWQVKNQGK"
     gene            complement(803..3142)
                     /locus_tag="VGC82_01905"
     CDS             complement(803..3142)
                     /locus_tag="VGC82_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY0422060.1"
                     /translation="MDEHHDHHHAGNVTDEVVTVIDPVCGMKVNAETSRHRFQHTGKT
                     FHFCSAGCRTKFEADPDKYLRPRAPAPVEAGATYTCPMHPQIRQPGPGSCPICGMALE
                     PLELTAEAAPNHELIDMTRRFWIGLAFALPVFVLEMASHIPGLGKHDLVPPQASVWIQ
                     FALSTPVVFWAGWPFFQRGWASVVSRNLNMFSLIALGTGAAYLYSLVATFAPGSFPTG
                     FRGMDGMVAVYYEAAAVITVLVLLGQVLELRARERTGDAIRALLNLAPKTARRIPDSG
                     EDEDIAIDQVKVGDRLRVRPGEGIPVDGEVLDGSSAVDEAMVTGESMPAAKHTGDKLI
                     GGTVNGTGSLVMRAQKIGSDTILARIVAMVADAQRSRAPIQRLADIVAGWFVPAVLAV
                     AVLSFAAWAAWGPSPSLAYGLVAAVSVVIIACPCALGLATPMSIGVGIGKGAGAGVLI
                     RSAEALERLEKVDTIVVDKTGTLTEGKPSVIEVITAPGTSRARLLALAASLEQSSEHP
                     LAAAIMAAAKAERVTVPAAADFASATGKGVTGKAGGQAVALGNAGLMADLGVGLDDLE
                     GRADALRRDGATALFVGVDGRATGIIAVADPIKATTRAAIGALKASGLHIVMLTGDNK
                     TTAEAVARALGIDDVRADVLPDDKHRIVKALKANGKIVAMAGDGVNDAPALAEADVGI
                     AMGTGTDVAIQSAGITLVKGDLAGIVRARTLSRMTMRNIRQNLVFAFAYNIVGIPVAA
                     GILYPAFGILLSPVVAALAMSLSSVSVITNALRLRAARL"
     gene            3289..3744
                     /gene="cueR"
                     /locus_tag="VGC82_01910"
     CDS             3289..3744
                     /gene="cueR"
                     /locus_tag="VGC82_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HEY0422061.1"
                     /translation="MNIGEAAKASGVTAKMIRYYEDVGLTPPAERSRAGYRSYNDRDV
                     DTLRFIRRARDFGVSMDQVRLLVGLWQNKNRPSREVKEIALRQVAELTAKIAELTAMK
                     DALGELADACRGDSRPDCPILKDLAGVALTPSHPGPASRSRKSGQRFAG"
     gene            3879..4127
                     /locus_tag="VGC82_01915"
     CDS             3879..4127
                     /locus_tag="VGC82_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422062.1"
                     /translation="MRTAILGLALLGAVTIGTSSQASSLSGNSPTVQPVYWNGDYCGP
                     RCQEHQWRRHERWEARRHAWQFRRGEERRYGYYAHPRY"
     gene            4400..5974
                     /locus_tag="VGC82_01920"
     CDS             4400..5974
                     /locus_tag="VGC82_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422063.1"
                     /translation="MGLFSERERQIRATRRELLALAGLGFGLAAGSGRAAGSSPQGQL
                     TYGIHVSLAPTWFDPAETTGLITPFMVLYAVHDGLVKAMPGQLQAPSLAESWSATEDG
                     LTYDFVLRNDVLFHNGEKVTADDVKFSFARYRGSDHSLLKQRVEAIETPDPRHVRIKL
                     KQPWPDFLTFYTGATGAGWIVPRKYVEKVGDDGFRKAPIGAGPYKFVSFNPGVELVLE
                     AFEPYWRNTPQVKRLVLKVIPDESTRLAALKRGEIDIAYSIRGELAEELRQTSGLTLK
                     PVVVQGTFCVYFADQWDPKSPWHDQRVRLAASLAIDRETINQALTMGQSHITGSAIFP
                     DTFEFYWQPPTPVYDPKQAKQLLAAAGFPNGFDAGSYFCDSSYTNIAEAVVNNLLEVG
                     IRTQLRPIERAGFIKGYTEKTYKNLIQAGPGAFGNAATRLESLVVQGGPFTYGSYPDI
                     DALFPQQAVELDHGRREAILHKMQQMVHERTMYAPIWQLAFINGVGPRVAESSFGRIA
                     GFPYTAPYEDLVLKGT"
     gene            6259..6570
                     /locus_tag="VGC82_01925"
     CDS             6259..6570
                     /locus_tag="VGC82_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY0422064.1"
                     /translation="MIDTPSRAERLHTALTRAFAPTILQVQDDSAQHEGHAGAQAGGQ
                     THYSVLLVSVAFQGVNRVARSRAVHSALAAEFGPAEQGGMHALALTLRTPEEHAAQAG
                     R"
     gene            6682..7146
                     /locus_tag="VGC82_01930"
     CDS             6682..7146
                     /locus_tag="VGC82_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0422065.1"
                     /translation="MVVDKDIDRLDREILRLISVDASLSLADIAAKVGLTPTPCWKRI
                     RRMEQSGIIQRRVAILDADKVGLPVSVFVAVETADHSSEWLSRFAKVIQDMPEIVDAW
                     RMSGDVDYLLHVVVADIASYDQFYRRLIGAVPLRNVSSRFSMERMKAAPLPV"
     gene            7383..7679
                     /locus_tag="VGC82_01935"
     CDS             7383..7679
                     /locus_tag="VGC82_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEY0422066.1"
                     /translation="MTISRLVNRNVVAERGRTSMRLEPELWDMLSEICEREAQDMSTL
                     VRQIEAAGHAGGRTSAVRVYIANYFKAAATEVGHATVGHGPILRRLPELTRSAA"
BASE COUNT         1512 a         2439 c         2471 g         1514 t
ORIGIN      
        1 acgctagaat gtgcggcacc aaaggaaaag gccgagcgcc atacggcggc gatcgagtga
       61 aggagtgcgt ggcaactggc caagatgcgc tgaatctatg ctggcgcgat caagcacatg
      121 acgcgtaacc tttgcgaacc gcaaccagcc aggacccaca tgcccgcggc gcaaggccgc
      181 ggctgggacg atgactccca acagccctcg ctgcccgggt ggatgttcac ctctaccaat
      241 gacccttaaa gttgacctgc aagggcaggc caacggccgc tggcataaga attgttcttg
      301 ctcgccgccc accaacccgg cgcgatacca acggccgtta ctgatcgaag ggccatcagt
      361 attagtaagg gccgttggca ttacttccct tggttcttga cctgccacgc cttcatctgg
      421 gcgatctcct tttcctgcgc ctggacgatg gccgttgcca gccgtcgtag ttccttgtct
      481 ttaccatatt gcaattcgac cttggccatt tcgatggcac cccgatgatg cggcagcatg
      541 ccggcgacaa aatcccgatc ggcatcgccg gtcatcggtg ccgccatgtc gtgcatcatc
      601 ctctcatccg cggccctgaa ggcgtcggac gacgggctgc gacccgtgcc gtgcgtggac
      661 atctccggcg acggggacga agcattggaa tgcggcggtt gggcggaagc tgggaccaaa
      721 gctgccccga caatcagggc ggcagcacaa agcgtggcct tcattttgta actcctgtgc
      781 aggggcgggg tcagcgggtt tatcacagcc tggcagctcg cagccgcaag gcgttggtaa
      841 tgaccgacac cgaacttagc gacatcgcca aagcggcgac cacggggctc agcaggatac
      901 cgaacgccgg gtaaagaatc cccgcggcga cgggaatgcc gacaatgttg taggcgaacg
      961 caaacaccag gttctggcgg atgtttcgca tggtcattcg gcttaatgtc cgcgcccgga
     1021 caatgccagc gaggtcaccc ttcaccaagg tgatgccggc gctctggatg gccacatccg
     1081 taccggttcc catggcgatc ccgacatcgg cttccgccag cgcaggcgcg tcgttcacgc
     1141 cgtcgcctgc catggcgaca attttgccgt tcgccttcag cgccttgacg atccgatgct
     1201 tgtcatctgg cagcacgtcg gcgcgcacat cgtcgatccc aagcgcacga gcgaccgctt
     1261 cggcggtcgt tttgttgtcc cctgtcagca tcacgatgtg caagccgctc gcctttagcg
     1321 caccgatagc ggcgcgcgtg gtcgccttga tggggtccgc gaccgcaatt atcccggtcg
     1381 cccggccatc cacaccgacg aacagcgccg tcgctccatc gcgacgcagt gcgtccgccc
     1441 gaccctcaag atcatccagg ccgacaccca gatcggccat gagaccggcg ttgcccaggg
     1501 cgacggcttg gccgcccgcc ttgccggtaa cacctttgcc ggtggccgag gcaaaatcag
     1561 cggccgctgg aaccgtgaca cgttccgcct tcgccgcggc cataatcgcg gctgccagtg
     1621 gatgttcgct cgactgctca aggctcgccg ccaaggcgag caagcgggca cgcgatgtgc
     1681 ctggggcagt gatcacctcg atgacggagg gctttccctc ggtcagcgtg ccggtcttgt
     1741 caacgacaat ggtatcgacc ttctccagcc gctccagcgc ttcggccgaa cggatcagca
     1801 cgccggcacc cgcgccctta ccaatgccga ctccgatcga catcggcgtc gccaacccca
     1861 atgcgcaagg acaggcgatg atgacgacag agaccgccgc gaccagacca taagccagcg
     1921 acggggatgg tccccaggcc gcccaggcgg cgaacgagag cacagcgaca gcgagcaccg
     1981 ccgggacaaa ccagcccgct acaatatccg ccaaccgctg gatcggtgct cggctgcgct
     2041 gcgcgtccgc caccatcgcc acgatgcgcg ccagaatggt gtcggaaccg attttttgcg
     2101 cgcgcatcac cagtgagccg gtgccgttca cggtgccgcc gatcagcttg tcacccgtat
     2161 gtttggcagc gggcatggac tcgcccgtca ccatcgcctc atcgacggcg ctgctgccgt
     2221 cgagcacttc tccatccacc gggatgcctt cgccggggcg aacgcgcagc cggtcgccga
     2281 ccttcacctg gtcgatcgcg atgtcctcgt cttcgccact gtcggggatc cggcgggccg
     2341 ttttaggtgc caggttcagc agggcgcgaa tggcgtctcc ggtccgctcg cgggcgcgca
     2401 gttccagcac ctgcccaagc agtactaaga cagtgatgac cgcggcggcc tcgtaataga
     2461 cggcgaccat cccatccatg ccgcgaaacc cggttgggaa gctgccgggc gcaaacgtgg
     2521 cgaccaggct gtagagatag gccgcgcccg ttcccaatgc gatcagcgag aacatgttga
     2581 ggttgcggct gacaaccgag gcccagccac gctggaaaaa cggccagccc gcccagaaaa
     2641 cgaccggcgt ggacagggcg aactgaatcc agacggaggc ctgaggcgga accaaatcat
     2701 gcttgccgag acccgggatg tggctggcca tctccaggac gaacaccggc agggcgaacg
     2761 cgaggccgat ccagaagcgg cgcgtcatgt cgatcagttc gtggttcggc gccgcctcgg
     2821 cggtcagttc gagtggttcg agcgccatac cgcagatcgg acagcttccg ggtcccggct
     2881 gccggatttg cggatgcatc ggacaggtgt aggtggcgcc ggcttctacg ggtgccggcg
     2941 cccgcggccg gaggtatttg tccggatcgg cctcgaattt cgttctgcat ccggccgagc
     3001 agaaatggaa tgttttgccg gtatgctgaa agcggtgcct ggaggtttcg gcgtttacct
     3061 tcatcccgca cacgggatcg atcaccgtga ctacttcatc tgtaacgttt ccagcatggt
     3121 gatggtcatg atgttcgtcc acggcgcgtc tccctgactg accggttttg ccggatcggg
     3181 tgggtcggga ccaacatggg gcttcccacc gtgggaaggt caagccttac cgcgggcatc
     3241 ttgtgcttcc catggtggga acctcataag ggccgcgggg aggggtgcat gaacattggt
     3301 gaggcggcca aagcgtcggg ggttaccgcc aagatgatcc ggtattacga ggatgtgggc
     3361 ctgacccctc cggccgagcg aagcagggca ggctaccgga gctacaacga ccgcgatgtc
     3421 gatacgctgc ggtttatccg gcgcgcgcgg gacttcggcg tgtcgatgga tcaagtgagg
     3481 ctgctggtcg ggttgtggca gaacaagaac cggccgagcc gcgaagtgaa ggagatcgcc
     3541 ttacgtcaag tggcggagct gacggcgaag atcgcagaac taacggcgat gaaggatgcg
     3601 ctcggtgagc tggccgatgc ctgccgtggc gatagccgcc cggattgtcc gatcctgaag
     3661 gatctggcgg gggttgcttt gacgccgtcg catcccgggc cggcttcgcg ttccaggaaa
     3721 tccggccagc gcttcgcggg ctgatattgc gctggggcgc gactggtcaa agtggcgtga
     3781 gagcaaccga tggggtccgt tatcctattt ttgacaccgt tgcgcccagt cttcgcctca
     3841 gctcattccc aactccttcg cgggaaggag gacaagacat gcgtaccgcg atactgggat
     3901 tggcgcttct aggcgccgtg acgatcggaa catcctcaca ggcgagcagc ctgtctggaa
     3961 attccccaac ggtgcaaccg gtttactgga acggcgacta ttgtggccca cggtgccagg
     4021 agcatcagtg gcggcgacat gagcgttggg aggctcgccg ccatgcctgg caattccgcc
     4081 ggggggagga acgccgctac ggctattacg cacatccccg ctattaggag gcgttcatgc
     4141 gagcggtttt gggactgttt ctgctgatcc tgatcggcgg atcggtggcg ggatgtgtca
     4201 tcgaagaacc agggtggcgc caccaccatc acttcgaacg ctattaaatc tcggtcgatg
     4261 cttccccgat tccgattgcc cgcttaaagc cgggatcggg cgcaccggca ccgccaagca
     4321 gatagggctg ccggatcgag taaagctcct ctaagctggt cacaggtctc cgccagagag
     4381 atcaaccagg gagaggacaa tgggcttatt ttcagagcgg gagcgacaaa tccgcgcgac
     4441 gcggcgtgaa ctacttgccc ttgccggcct gggcttcggg ctcgcggctg gctcgggccg
     4501 ggcggccggg agctctccgc agggacagtt gacctacggc atacatgttt cccttgctcc
     4561 gacatggttc gatccggcgg aaaccacggg gttgatcacc ccgttcatgg tgctgtacgc
     4621 ggtacatgac ggcctggtga aggcgatgcc cgggcaactc caggcgccga gcctcgccga
     4681 gtcgtggtcg gccacggagg atggtcttac ctatgacttc gtcctgcgga acgacgtgct
     4741 gttccataat ggggagaagg tgacggcgga cgatgtgaag ttctcattcg cacgctaccg
     4801 agggtccgac cacagcctgc tgaagcagcg cgtcgaggcg atcgaaacgc ccgatccgcg
     4861 gcatgtgcgc atcaagctga aacagccatg gccggacttc ctgacgtttt ataccggcgc
     4921 caccggtgcc ggctggatcg tgccgcggaa atacgtcgag aaggtcggtg acgacgggtt
     4981 caggaaagcg cctattggtg cgggacctta caagttcgtc tcgttcaatc ctggtgtcga
     5041 gctggtgttg gaagcattcg agccgtactg gcgcaacacg ccccaggtaa agcgtctggt
     5101 gctgaaggtg atcccggacg agtcgacgcg actggccgca ctaaagcgag gcgagatcga
     5161 catcgcctat tcgattcgcg gcgaactcgc cgaggaattg cggcaaacct ccggcctgac
     5221 cctgaagccg gtcgtcgtgc agggcacctt ttgtgtttac ttcgcagatc aatgggaccc
     5281 gaaatcgccg tggcacgacc agcgggtgcg gttggccgcc agtctcgcca tcgaccgcga
     5341 gaccatcaac caggcgctga ccatggggca atcccacatc accggcagtg ccatctttcc
     5401 ggacacgttc gagttctatt ggcagccgcc cacaccggtg tacgacccga agcaggccaa
     5461 acagttgctg gcagccgccg gttttcccaa cggcttcgat gcgggctcct atttctgcga
     5521 ctcctcgtac accaacatcg ccgaggcggt ggtcaacaac ctgctggaag tcggcatccg
     5581 cacccagcta cggccgatcg agcgcgccgg tttcatcaag ggctacaccg agaagaccta
     5641 taagaacttg attcaggccg gacctggcgc gttcggcaac gcagcgaccc gtcttgagag
     5701 cctggtcgtg caaggcggcc cgtttaccta tggcagctat cccgacatcg acgcgctgtt
     5761 tccgcaacag gccgtggaat tggaccatgg ccggcgtgaa gcgatcctgc acaaaatgca
     5821 gcaaatggtg cacgagcgga cgatgtatgc gccgatctgg cagctcgcgt tcatcaacgg
     5881 cgtcgggccg cgggtggcgg agtccagctt cggacggatc gccgggtttc cctataccgc
     5941 gccatacgag gacctggtct tgaagggcac ctgacggctt cttgccccga cttgccgggc
     6001 acgctggacg gacgttgcgg cgtccgatcc agcacccgac cccgatggcg aaagatccgt
     6061 ccatggcgtg cgacgggttg aacttgcgta aggtgtccag aggcccaatc aaacccgtag
     6121 caagggcgtg cataatcgcc agtcaatagc aagattggct ccaagacggt tttcggttcg
     6181 gtttcgagct ccagcgcatt cgcccagctg gttgcattgc ggctttgcgg ccgatgcaaa
     6241 agattggata atcaggccat gatcgataca ccctcccgcg ccgaacgact gcacacggca
     6301 ctcacccggg cgttcgcgcc aaccatcctg caggtgcagg atgatagtgc ccagcacgag
     6361 ggccatgccg gcgcacaggc cgggggccag acccactaca gcgtgctgct agtcagcgtt
     6421 gcgttccagg gcgtcaaccg ggtcgcacgc tctcgcgccg tgcacagcgc gttggcggct
     6481 gagtttgggc cggccgaaca gggcggtatg catgcactgg ccctgactct gcgcacgcct
     6541 gaggaacatg ccgcccaggc aggaagataa ttttctggta tccctcagat ccggaatttc
     6601 gattattttc ttctccagat ggacgcgtta aggatcgacc tcaaaaaatt tttgctgggt
     6661 ttttgaggaa ggctatgccg catggttgtg gacaaggata tcgaccgcct cgaccgtgag
     6721 atccttcgct tgatatcggt ggatgcctcg ctgtccttgg ccgacatcgc ggcgaaggtt
     6781 gggctgaccc cgacgccgtg ttggaagcgc attcgccgga tggagcagag tggcatcatc
     6841 cagcgccggg tcgccatcct ggacgcggat aaggtcggcc taccggtctc ggtcttcgtt
     6901 gcggtcgaaa ccgccgatca ctccagtgaa tggctcagtc gcttcgccaa ggtgatccag
     6961 gacatgcctg aaatcgtcga tgcatggcgc atgagcggcg atgtcgatta cctgctgcac
     7021 gttgtcgtgg ccgacatcgc gtcgtacgat cagttctacc gcagattgat cggagcggtt
     7081 ccactgcgaa atgtcagcag ccggttcagt atggagcgaa tgaaggccgc ccctctgccg
     7141 gtgtaatccg cacttcccgc tgcagcgccg accatgttgc gcgcctgata acccgcgcgg
     7201 ctgttcgtct tgatgcgcat agcatcttct ggtgctgctg gtcgaccatg atctgcttca
     7261 acaccaaaca aatttcgacc gattgtgtgt attcggcgct aatctgtcca tattttttcg
     7321 ttttatcaac gttctattaa ctcatttctg gtttcatcgg cctcggcaat aggaggtggg
     7381 ccatgacaat cagtcggcta gtcaatcgta atgtcgtcgc tgagcgaggc cggaccagca
     7441 tgcggctgga gcccgagctc tgggatatgt tgagcgagat atgcgaacgc gaagcgcagg
     7501 atatgagcac tttggtgcgt caaatcgaag cggccgggca cgcagggggc cgcacaagcg
     7561 ctgtccgcgt ttatatcgcg aattacttca aagccgccgc gacggaggtg gggcacgcaa
     7621 ctgtcggtca cggcccgatc ctgcggagat taccggaact gacccgctcc gccgcttgac
     7681 catgacgaac gggcgaatcg ccgttccaat tcgaccgcag gtgccaacaa aatgctgggc
     7741 ttgcggcctt cggccccagt ggatcaggcc gcgctcgatt cctgcctggc caattcgcgc
     7801 ttcctgtaac ggacgcccgg cacgatatcc tggctcgcga tcgttatgat gcgatcgtta
     7861 tagcgggcgg gcgggtctga tcaacgcttg atcgcatcga tggccgactg gatcattttc
     7921 gcgtcgtcgc cgccct
//
LOCUS       DASWQN010000117         3210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_13332, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000117 DASWQN010000000
VERSION     DASWQN010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13332"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..269
                     /locus_tag="VGC82_01940"
                     /pseudo
     CDS             24..269
                     /locus_tag="VGC82_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492304.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
     gene            complement(372..1499)
                     /locus_tag="VGC82_01945"
     CDS             complement(372..1499)
                     /locus_tag="VGC82_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="depolymerase"
                     /protein_id="HEY0422067.1"
                     /translation="MEQTKLVTAARHAARALLIATALGFATRAWAAPPLGRYRVDPTQ
                     VSVSGISSGAFMANQLHIAHSAGIMGAAMIAGGLYGCPVLAVQQDGVYALASQAIGAC
                     MSSPSLLNSVDGYQQTVNELATRGWIDPPSNLAYGRVYMFTGQADQVVNSKTVELGED
                     LYEALGVPVAQIKFDDRDLPGPGAGHSWVTSNFGKACDANASPYINNCHYDQAGIELQ
                     WIYGPLQKRAATPTGRIVAFDQTEFVPGHAAVANGMLDTGYLYVPKTCEPGTAQFCRL
                     QVALHGCLQSAEALKDEFYTRIGLNEWADTNRIIVLYPQAHATTVGELSRQDALSLVN
                     ANPKGCWNWWGYAYDTQFLTKQGVQVSAIWSMIQRIDGQGN"
     gene            complement(1527..1922)
                     /locus_tag="VGC82_01950"
     CDS             complement(1527..1922)
                     /locus_tag="VGC82_01950"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY0422068.1"
                     /translation="MQMRNMRALSTIQQQMLDGLSMLAQQLSQITASSLRRAFGTAQA
                     ASSPSDLRELVGTRIDDLKSSILDAQANSNAISEIMARSLGEAASTLQTRTMAALDEC
                     KTLLEQVVPDSVSLARPGAASPMVVSQPN"
     gene            2770..3171
                     /locus_tag="VGC82_01955"
     CDS             2770..3171
                     /locus_tag="VGC82_01955"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY0422069.1"
                     /translation="MATRPAASKKAPARKAVAKQPAPAAAKSKSMTPAPKPKATAAPV
                     VTLKAVFEQLGEAHALQKKQAHTLLADFVAAVTTHLKQGARVRMSGLGILAIKTREAR
                     MGRNPATGESIQIKASRKVGFRPAKELKDAI"
BASE COUNT          593 a         1033 c          965 g          619 t
ORIGIN      
        1 taccgtaaca acaccataaa cgtttgataa gttttgtgaa tatccatgca ccacgctgac
       61 gtgcggagcc aatctcgacg gcggtcactg aaagcacggt gcaatggctg ccacaccggc
      121 ggatgaacgc ccagcagcag atgcgttgga cgccgcgcgg tgcgcacttg atgctcaagg
      181 ttcgatgcgc ggtgatgagc ggtactctgg aacacgatca cgctgttgcc gaagggcaag
      241 cctgtcgtcc cttccggcgc gcggcgtgac ccccaggttc gagacggtct ctatccgcat
      301 taatgaccgc aaacggggtt ggggtcgatg cgcctacacc cttgatccgt gcgcctcgct
      361 gccctgcgct gtcagttgcc ctgcccatcg atccgctgta tcatcgacca aatcgccgaa
      421 acctgcacgc cctgcttggt gaggaattgc gtgtcgtaag cataccccca ccaattccag
      481 caccccttcg gattggcatt caccagcgac agcgcgtcct gccgcgacag ttcgccgacc
      541 gttgtcgcat gagcctgcgg gtagagcacg ataatccggt tggtatcggc ccactcgttc
      601 aaaccgatcc tggtgtagaa ttcgtccttc agcgcctcgg cagactgcag gcagccgtgc
      661 agcgccacct gtaaacggca aaactgcgcg gtcccgggct cgcacgtctt ggggacatag
      721 agatatcccg tgtccagcat cccattcgcc accgccgcgt gtccgggcac gaattcggtt
      781 tggtcgaagg cgacgatccg tccggtcggt gtcgccgcac gcttctgcaa cggcccgtag
      841 atccactgca gttcgatccc ggcctgatcg tagtggcaat tgttaatgta cggcgaggcg
      901 ttggcgtcgc acgccttgcc gaaattgctg gtcacccagg agtgcccggc gccagggccg
      961 ggcaggtcgc gatcgtcgaa cttgatctgc gcgacgggca ctcccagcgc ctcataaagg
     1021 tcctcgccaa gctccaccgt ctttgaattg accacctggt cggcctgtcc cgtgaacatg
     1081 tatacgcggc catacgccag attggacggc ggatcgatcc aacctcgcgt ggccagctcg
     1141 ttgactgttt gctggtagcc atcgacgctg ttcagcagcg acggcgacga catgcaggcg
     1201 cctatggcct gcgacgccag cgcatagaca ccatcctgtt gcactgcgag gacggggcag
     1261 ccatacagcc cgcccgcgat catcgcggca cccatgatgc ctgcggaatg cgcgatgtga
     1321 agttggttag ccatgaacgc gccggacgag ataccggaca ccgacacctg cgtcggatcg
     1381 acccgatagc gcccgagcgg cggtgcggcc catgcgcggg tggcgaagcc taacgccgtg
     1441 gcgatcagta gcgcccgcgc cgcgtgacgc gcggccgtca ccaatttggt ctgttccatg
     1501 gaaatggttc cttcgcgtca ggcggcctaa ttcggctggc tcaccaccat ggggctggcc
     1561 gcgcccggcc gtgccagtga aacgctgtcc gggaccactt gctccagcag cgttttgcac
     1621 tcatcgagtg ccgccattgt ccgcgtctgc agggtgctgg cggcttcgcc gaggctgcgg
     1681 gccataatct ccgagatcgc gttggagttt gcttgcgcat ccaggatcga cgacttcaga
     1741 tcgtcgatgc gcgtgccgac tagctcgcgg agatccgatg gggaagatgc cgcttgggcg
     1801 gtgccgaagg cgcgacgcag cgacgatgcc gtgatctgcg atagctgttg ggccagcatc
     1861 gataagccgt caagcatctg ctgctgtatg gtcgagagcg cccgcatgtt gcgcatctgc
     1921 atgccgacca gttcggtgat acaacccatg ccgtgcggcg attgaacggt attatccata
     1981 tcgatactcc tcgtttttta agatttcgtg tgcctatcgg cagctctgga ccttgcggca
     2041 agccactgcc gcacaggcga gcagtctcgg ccagaacagt cgggtttaac ctggcattct
     2101 ctgcgcgtgc ggccgccatc tggttaggca ataggctgcg cctccgcgag cagcgccgtt
     2161 ttttgtgcgc tcccgacgct cgatccgacc cagtaggtgg catcccgagt gctgctaagc
     2221 aattgatatc atgcgtttct tgcgccccct gtgctttcga tggggagcat accgaggtat
     2281 agccggtcca tctgtgaacg ccatcacact cggcaggacg gccgtatcgc gacattccac
     2341 cgcagcattg atgaatgtcg ctttagctcc gctgtcttcg gacagccagc cgattaattc
     2401 gattaggcgc gataaccgct cttatcgcgc gtgggtgggg gaaacgtgaa gaacaacagc
     2461 agaaaacctc gctggaggcg caagccacgc cggcgacccc tgccctgccg ccgtccgcgg
     2521 cctggccagc ttctcgtcct acccatggcg gccgatgcgg cggtccgctc tcgcgtctgg
     2581 ctgacctcga tcctcgatcg tgttccgcgc cgcccgtggt gcgcgggccg ttgcttccag
     2641 ttggcattgt ccacggcagc atcggcgtct ggtgatgctc aacagggcgc gcggcagcgg
     2701 aaagtccctt gccggccagc cgattcgcgt gattgcgtcg gtcccaccac cacaagcgag
     2761 tttgtagtca tggccacccg ccctgccgcc agcaagaaag cccctgccag aaaggcggtt
     2821 gccaaacagc ctgcacccgc ggcggccaaa tcgaagtcca tgactcctgc ccccaaaccc
     2881 aaggccaccg ctgcgcccgt ggtcacgctg aaggcagtct tcgagcagct cggggaggcg
     2941 cacgccctgc aaaagaagca ggcccacact ctgctggcgg acttcgtggc cgcggtgact
     3001 acgcatctga agcagggcgc gcgggtccgc atgagcgggc tcggtatcct tgcaatcaaa
     3061 acccgcgagg cccgcatggg ccgcaatcca gccaccggtg aaagcatcca gatcaaggcc
     3121 agcaggaagg tgggatttcg cccagccaag gaactcaaag acgcgattta aatcgcgtcc
     3181 agtttggaaa ccgtagtctc tcgtcgtgcg
//
LOCUS       DASWQN010000118         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_409048, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000118 DASWQN010000000
VERSION     DASWQN010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409048"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VGC82_01960"
     CDS             complement(<1..449)
                     /locus_tag="VGC82_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0422070.1"
                     /translation="MNRTHQILIGSACALLLVLFSFTAPMLVNDYFVRVMTVMCINAI
                     LVLSMGLTNGFTGVFSLGQVGFVAVGAYVSGVLSLPAAAKAAYLPNLPGWIAVINLGF
                     FSSTIVAGLICILLAFLVGRPLMRLSGHFVSVATLGFLIIVNNVLINA"
     gene            complement(446..1348)
                     /locus_tag="VGC82_01965"
     CDS             complement(446..1348)
                     /locus_tag="VGC82_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0422071.1"
                     /translation="MSIELYIAQQLINAISLGSLYALVAIGLSLIFGILRMANFAHGD
                     MMMVGAFATLVLQWLGCDFGWAVIGGIAVGALAGVLVERVAYRPVRGAPEVTMLLTSF
                     AVTYILENLGILIFTSSPRNFPLPEWLNSAFQLFDGQITFTQINILTVVLTTVALAAL
                     GWFVTRTDVGLGMRAAAEDMPAAHLVGLDVNRLIIVAFAIASGFAGLAGILWAAQSGV
                     VEPHMGFTPLLKAFVGAIIGGFGSIPGAVIGGYLLGALEICIVAFMPSQVSPYRDAIV
                     FALLIAFLLVRPNGIMKPTQEIKL"
     gene            complement(1345..>1669)
                     /locus_tag="VGC82_01970"
     CDS             complement(1345..>1669)
                     /locus_tag="VGC82_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0422072.1"
                     /translation="PAGTLSGGEQQMLALGRALMAKPRLLLLDEPSLGLAPLLVKEIF
                     RKLAEFKAAGVTMLVVEQNINLALDLADRAYVLRTGEVSLAGPAVDLKADYEKVAAAY
                     LGVRP"
BASE COUNT          382 a          498 c          540 g          249 t
ORIGIN      
        1 gcattgatca gaacgttgtt gacgatgatg aggaaaccca gtgtcgccac ggatacgaag
       61 tggcctgaca gccgcatgag cggcctgccc accagaaagg cgagcaagat acagatgagg
      121 cccgccacga tggtcgaaga gaagaatccg agattgatga cggcgatcca accgggcaga
      181 tttggcaggt atgcggcctt tgccgcggca gggagcgaga gcacgcccga gacataggcg
      241 ccaacggcca cgaatcccac ctggcccagc gagaagactc ccgtgaaccc atttgtcagc
      301 cccatggaca acaccaggat ggcgttgatg cacatgaccg tcatgacacg cacgaaatag
      361 tcattcacga gcatcggcgc tgtgaagctg aataggacca aaagcagtgc acatgcggag
      421 ccgatgagaa tctggtgcgt gcggttcaca gcttgatctc ttgagtgggc ttcataatgc
      481 cattgggccg gaccaggagg aaggcaatga ggagcgcgaa gacgatggcg tcacgatagg
      541 gcgagacctg cgaaggcatg aaggcgacga tgcagatctc gagcgcaccg agcagatagc
      601 ctccgatgac ggcgcccggg atgctcccga accccccgat aatggcaccg acgaaggcct
      661 tgaggagcgg cgtgaagccc atatgcggct cgaccacgcc cgattgggcg gcccagagaa
      721 tccccgcgag acccgcgaaa cccgaagcga tggcgaaagc gacgatgatc agccggttga
      781 cgtcaaggcc gaccaggtgc gcggccggca tgtcctcggc ggccgcgcgc atgcccagcc
      841 cgacgtctgt ccgggtgacg aaccagccga gcgcggcgag cgctaccgtc gtcagcacca
      901 cagtcaggat gttgatctgg gtgaaggtga tttggccgtc gaagagctgg aaggcgctgt
      961 tcagccactc gggaagcgga aagttgcggg gtgaactggt aaagatgagg atgccgaggt
     1021 tctccaggat ataggtgacg gcgaagctgg tgagcagcat cgtcacttcc ggtgcgccgc
     1081 gcaccggtcg ataggcgacc cgctcgacca gcacaccggc gagcgcaccg acggcaatgc
     1141 caccgatgac ggcccagcca aaatcgcagc cgagccattg cagcaccagc gtcgcgaagg
     1201 ctcccaccat catcatgtcg ccatgggcga aattggccat gcgcaagatg ccgaagatca
     1261 gcgatagccc aatggcaacg agcgcataaa ggcttccaag gctgatcgcg ttgatgagct
     1321 gctgcgcgat gtagagctcg atactcatgg ccgcactccg agataggcgg ccgccacctt
     1381 ttcatagtcg gccttgaggt ccaccgccgg tcccgcgagg ctgacctctc cggtgcgcag
     1441 cacataggcg cgatcggcga gatcgagggc caggttgatg ttctgctcga ccaccagcat
     1501 ggtgacgcca gcggccttga actcggccag cttacggaat atctccttga ccagcagcgg
     1561 cgccagaccc agggacggct cgtcgagcag cagcaaccgt ggcttggcca tgagggcgcg
     1621 gccgagggca agcatctgct gctccccgcc cgaaagcgta cctgcgggc
//
LOCUS       DASWQN010000119         6394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2256512, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000119 DASWQN010000000
VERSION     DASWQN010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2256512"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VGC82_01975"
     CDS             complement(<1..905)
                     /locus_tag="VGC82_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422073.1"
                     /translation="MKNAKPPSVVSGEGLPSTPSVGAAMPVEGRAAATTPAGTLSRRT
                     AIGIGLAAGSATIVSRAARAQAPAEVKIAMLVPLSGPWARQGILEQMGARLAIEDING
                     AGGIKSMGGAKLKLIEYDTQDSAEKAKDAAQRMVAQEPDLVGGFGCWLSTFTLAATEV
                     TERVELPWLTLSYSDMITGRGFKHVFQSSPTADRQAEELLPMIVELAQKASGKKPTKF
                     AYIGDNTASSVSFMKSIRGQSAKDLGLTLVADEVYTPPLADATTMIQHIRSARPDFVG
                     FQSTNVPDDKLLADKFAEFGMKSDK"
     gene            complement(936..1646)
                     /locus_tag="VGC82_01980"
     CDS             complement(936..1646)
                     /locus_tag="VGC82_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0422074.1"
                     /translation="MLEVVNLSVSYGGLRALNDVSLSVREGQFVTVVGPNGAGKSTLF
                     KTISGIVPATSGQITFMGEDLFRLAPARRAHLGIAHVPEGRQVFKTLTVLENLEMGAY
                     PKAGREAWHHTLDRIHTLFPILSERAAQLAGTLSGGEQQMVAIGRGLACAPRLLMLDE
                     PSMGLAPAVADTIFERVSQIHREDGVTVLLVEQRVAEALELSDHGYVLETGHMVLDGP
                     YETLLRDDRVRKSYLGLS"
     gene            complement(1639..3405)
                     /locus_tag="VGC82_01985"
     CDS             complement(1639..3405)
                     /locus_tag="VGC82_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEY0422075.1"
                     /translation="MRYGSAPRLRRDLITLGIVSAAYLAVSTLVSNSYYQLILTLVPI
                     WAAFGVSWNILSGYGGQLSFGHASFFGIGAYVVTLALVYWNLTPWLGIPLGMIVAGCA
                     AVLIGLPTFRLRGHYFALSMLAYPLAILYFLQYLGFQEMSLPMHRENPIAYLEFTNPH
                     YYTVVAVLLLVAGLFVCMVVENSRFGLALLAIRQNELAAEAAGINTRLWKMRALVVSG
                     MIAASAGGFYACVLLVVTPDSVFGMLVSAQAVVVTLFGGVASIWGPVIGAAILVPLAE
                     SLNAEIGNYLPGIQGVVYGFAMIGIMLVSPDGLFWTLRDRFFTPKAAAPLPASLAAGA
                     PAMARGAAAGTTLLKVEALSRSFGGLRAVSQVSFEVYAGEILGIIGPNGAGKTTLFNV
                     LNGVLPPDEGSAILGGESMFGKKTFQVCRMGVGRTFQVVRSFPRLPLLDNVIVGAYGA
                     GFTDADAIASAAHALTRVGLGDQAATPAGQLTNKQLRLMELARALAGAPRLLLLDETL
                     AGLGREECDDVLDVLVRLRGEGMTVVIIEHTMHAMMRIADRFVVLDHGRVLASGLPRH
                     VVEDRSVIEAYLGKKWAAKQDA"
     gene            complement(3405..4361)
                     /locus_tag="VGC82_01990"
     CDS             complement(3405..4361)
                     /locus_tag="VGC82_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0422076.1"
                     /translation="MDLEGLIENVLNALAAGITIGCIYGLMCIGLGLIFGIMRIVNFA
                     QGELLMLGMFSSLYLVTGGGVLAFLGPYIGPFVGALLAGPIVFAGGALLHKFLIARVS
                     GLRTTGSLDEGHFGQLIVTLGISLILQNGGLIVFGSTPMTVRTPLASSAIEVGPLYGD
                     ATVFLNKARLIACAVAIIVAVSLYLLLEFTRMGKALRAAADNPTAATYMGIDTDKAYR
                     IAFGLGSGITAIAGGLMATYLSFGPFIGLDYVIIMYSGVVLGGMGSILGAFWGGLTVG
                     FVQQCSALILPPQLQNAAIFVVFLMIVLLRPQGLFGRSAERA"
     gene            4594..6006
                     /locus_tag="VGC82_01995"
     CDS             4594..6006
                     /locus_tag="VGC82_01995"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEY0422077.1"
                     /translation="MPTTQANSAAAPRSWNPNSWRDCPIRQVPTYPDLTKLAEMETRL
                     GKYPPLVFAGEARRLKAQLALAAEGRAFVLQGGDCAESFSDFTANVIRDTFRVLLQMS
                     VVLTFGASLPVVKMGRMAGQFAKPRSSDSETQGAVTLPSYRGDIVNGPEFTPESRIPD
                     PARMEFGYFQSASTLNLLRAFSSGGYADLHEVHRWNLDFVDRSPLAERYRDLSSRIDE
                     TLSFMAACGMTSATTRDLRETDFYISHEALLLPYEQALARVDSTSGDWYGCSAHFLWI
                     GDRTRQAEGAHVEFLRGLQNPLGMKVGPTQEADDLLKLLDILNPKNEAGRITLISRMG
                     ADKVEAKLTPLVRAVERSGHKVVWLCDPMHGNTISTSSKMKTRNFDSILHEVRSFFDI
                     HAAEGTWAGGVHVEMTGQDVTECIGGAHRLSEADLGGRYETFCDPRLNAEQSLELAFL
                     VAEELKTRRAPAAAPAIAAQ"
     gene            6016..>6394
                     /locus_tag="VGC82_02000"
     CDS             6016..>6394
                     /locus_tag="VGC82_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY0422078.1"
                     /translation="MAQAGDHEGRDLAADIAARTDSYFNRTKTIVARFGDRQVTYAVF
                     LRRPVICAPRLMLDWLKMVAELRATDIDVDLIHQEGDWVGAGEPIAYLTGSLVHLSDL
                     ETILLQKVGPVCVAAHNAYQMALS"
BASE COUNT         1269 a         2085 c         1967 g         1073 t
ORIGIN      
        1 atcttgtcgc tcttcatgcc aaactcggcg aacttgtccg ccagcagctt gtcgtccggg
       61 acattcgttg actggaagcc gacgaaatcc gggcgcgccg agcggatgtg ctggatcatc
      121 gtcgtggcgt cggccagcgg cggcgtatag acctcatcgg cgacaagggt cagccccaga
      181 tcctttgccg actggccacg gatcgacttc atgaagctga cggacgacgc ggtgttgtcg
      241 ccgatatagg caaacttcgt cggcttcttg ccggacgcct tctgggcaag ttcgacgatc
      301 atcggcagaa gttcctccgc ctggcggtcg gcggtcgggg acgactgaaa aacgtgcttg
      361 aagcctcggc cagtgatcat gtcggaataa gacagcgtca accatggcag ttcgacgcgt
      421 tcggtaacct cggtcgcggc cagagtaaag gttgacagcc agcagccaaa gccgccgact
      481 aagtctggct cctgtgcgac catgcgctgg gcggcatcct tggccttctc cgcactgtcc
      541 tgggtgtcgt attcgatcag cttcaacttg gcgccaccca tcgacttgat accgccggcg
      601 ccattgatgt cctcgatcgc cagcctcgca cccatctgct ccaaaatgcc ttggcgcgcc
      661 cagggtccgg acagcggcac caacatcgcg atctttacct ctgcgggcgc ttgtgctcgg
      721 gccgcgcgag acacgatcgt tgcgctgccg gcggcaagtc cgatcccaat ggctgttcgg
      781 cgggataggg tgcccgctgg ggtcgtggcg gctgcacgac cctcgacagg catggctgct
      841 ccaacagaag gcgtggatgg caggccttcc ccggacacga cggatggggg cttggcattt
      901 ttcatggcgc ttctccggtt cttggtggcc aagcgtcagc tcagcccgag gtaggatttc
      961 cgcacgcggt cgtcccgcaa caaagtctca tagggtccgt cgagcaccat gtgaccggtt
     1021 tccagcacat agccatggtc ggacaattcc agtgcctcgg ccacccgttg ctcgaccagc
     1081 aggacggtga caccgtcttc cctgtggatc tgcgagaccc gttcgaagat cgtgtcggcc
     1141 accgcgggtg cgaggcccat cgacggctcg tccagcatca gcaaccgcgg tgcgcaggcc
     1201 agaccgcggc cgatcgccac catctgctgc tcgccaccgg aaagcgtgcc ggcaagctgt
     1261 gccgcccgtt cggacaggat cgggaacagc gtgtggatgc ggtccaacgt gtggtgccac
     1321 gcttcccgcc cggccttcgg ataagcgccc atctccaggt tttccagcac cgtcagggtc
     1381 ttgaacacct gccggccctc cgggacatgg gcgataccca gatgcgcccg ccgcgccgga
     1441 gccaagcgga aaagatcctc ccccatgaag gtgatctgcc cggaagtcgc cggcacgatg
     1501 cccgagatcg tcttgaacaa ggtggacttt ccggcaccgt tcggcccgac gacagtgacg
     1561 aactggccct cccgcaccga caacgagacg tcgttcagcg cgcgcagccc gccgtaggag
     1621 accgatagat taacaacctc aagcatcctg tttcgccgcc catttcttgc cgagataggc
     1681 ctcgatgacg ctgcgatcct ccactacatg ccgcggcaag ccgctggcca ggacccgccc
     1741 atggtccagc accacgaagc ggtcggcgat ccgcatcatc gcgtgcatgg tgtgctcgat
     1801 gatgacgacg gtcatgcctt cgccgcgcag gcgcaccaac acatccagca cgtcgtcgca
     1861 ttcctcgcgc cccagccccg ccaacgtttc atccagcaac aacagccgcg gcgcccctgc
     1921 caacgctcgt gccagttcca tcagccggag ttgcttgttg gtgagctggc cggctggcgt
     1981 cgcggcttgg tctccaagcc cgacacgcgt cagcgcatgc gcggccgagg caatcgcatc
     2041 ggcatcggtg aaaccggcgc cataggcccc gacgatcacg ttgtcgagca gcggcaggcg
     2101 tgggaaactg cgtaccacct ggaacgtgcg gcccacgccc atgcggcaga cctgaaaagt
     2161 ctttttcccg aacatcgact cgccgccgag gatggcgctg ccctcgtcgg gcggcaggac
     2221 gccgttcagc acattgaaca aggttgtctt gccggcaccg ttgggaccga taatgcccag
     2281 gatctcgccg gcataaacct cgaagctgac ctggctgacg gcgcgcaggc cgccaaacga
     2341 gcgtgacagg gcctcgacct tcagcagcgt cgtccccgca gccgcaccac gcgccatggc
     2401 cggggcgcct gctgccagcg acgccggcag cggggcagcc gccttcggtg tgaagaaccg
     2461 gtcgcgtaag gtccagaaca acccgtcggg cgagaccagc atgatgccga tcatcgcaaa
     2521 gccataaacc acgccctgga tgccaggcag gtaattgccg atttccgcat tgagggattc
     2581 ggccagcggc accaggatgg ccgcgccgat gaccggcccc cagatggagg cgacaccgcc
     2641 gaacagcgtc acgaccaccg cttgcgcgga caccaacatg ccgaacacgg aatccggtgt
     2701 gaccaccagc agtacacagg catagaaccc gccggccgag gccgcgatca tgcccgatac
     2761 gaccagcgcc cgcatcttcc acagccgcgt gttgatgccg gcggcctcgg ctgccagttc
     2821 gttttgcctg atcgccagta aagcaaggcc gaaacgggag ttttccacga ccatgcagac
     2881 gaacaggccc gcgaccagca gcaagacggc gaccaccgta tagtaatgcg ggttggtgaa
     2941 ctccaggtat gcgatcgggt tctcccgatg catcggcagg gacatctcct ggaagccgag
     3001 atactgcagg aagtacagga tcgccagcgg gtaggcgagc atcgacagtg cgaagtaatg
     3061 acccctcagg cggaaggtcg gcagcccgat cagcaccgcg gcgcaaccgg cgacgatcat
     3121 ccccagcggg atgcccagcc agggcgtcag gttccagtaa accaacgcca gagtaacgac
     3181 gtaggcaccg atgccgaaga aggacgcgtg gccgaacgac aactgaccgc cgtagccgga
     3241 cagaatattc caggacacac cgaacgcggc ccagatcggc accaaggtca ggatcaactg
     3301 atagtagctg ttggaaacca gcgtcgaaac cgccagatag gccgccgaca cgatgcccag
     3361 cgtgatcagg tcgcgccgaa gccttggggc ggagccatag cgcatcaggc gcgctccgcc
     3421 gaccggccga acaggccctg aggacgcagc aggacgatca tcaggaagac cacgaagatt
     3481 gccgcgttct gcagttgcgg cggcaggatc agggccgaac attgttgcac gaacccgact
     3541 gtaaggccgc cccagaacgc gcccaggatg ctgcccatgc cgcccagcac gacgccagaa
     3601 tacatgatga tgacgtagtc caggccgatg aacggaccga acgacaggta tgtggccatc
     3661 agtccgccgg cgatggcggt gatgccgctg cccagtccga acgcgatgcg ataagccttg
     3721 tcggtgtcga tgcccatgta ggttgccgcc gtcgggttgt cggccgccgc gcgcagcgct
     3781 tttcccatgc gggtaaattc aagcagcaga tacagcgaca ccgcgacgat gatcgccacg
     3841 gcgcaggcga tcaaccttgc tttgttgaga aacaccgtgg cgtcgccgta gagcggaccc
     3901 acttcgatcg cactggaggc caacggcgtg cgcaccgtca tcggcgtgga gccgaacacg
     3961 atcaggccgc cattctgcag gatcagcgag atccccagcg tcacgatcaa ctggccgaaa
     4021 tgtccctcgt cgagggaacc ggtggtgcgc aggccggaca cgcgggcgat caagaatttg
     4081 tgcaacagag cgcctcccgc gaagacaata ggtcccgcca gcagagcgcc cacgaatggg
     4141 ccgatatagg gtccgagaaa ggccagcacg ccgccgccgg tcaccaaata aaggctggag
     4201 aacataccga gcatcagcag ctcgccttgg gcgaagttga cgatgcgcat gatgccgaag
     4261 atcagcccaa ggccgatgca catcagtccg tagatacagc cgatcgtgat ccccgcggcg
     4321 agcgcgttca gcacgttctc gataagacct tcaaggtcca ctggttccgc tgcccccgaa
     4381 ccggctgccc agttgggctg cttctgtttc gggtggtgtc ttaaccggtg ttgagccctg
     4441 gctcaagcgg cggggccgat ggcgcggtag ggcagtctcg gcttgttccg aaaggggcag
     4501 cttctggcgc gtcatccaag cggagcagcc gatttgcgga agcagtagcc atttccgccg
     4561 attcgcgaca taaagcagcc tgaggagatc gttatgccca ccactcaagc caacagcgct
     4621 gctgccccgc gttcctggaa ccccaattcc tggcgcgact gtcctatccg tcaggtcccc
     4681 acctatcccg acctgacaaa actggcggag atggaaacac gcctgggcaa gtatccgccg
     4741 ctggtgtttg ccggggaggc gcgccgtctg aaagcccaac tggcgcttgc cgccgagggc
     4801 cgcgcgttcg tactgcaagg cggcgactgc gcggaaagct tctccgactt caccgccaac
     4861 gtcatacgcg acacctttcg ggtgttgttg cagatgtcgg tcgtgctgac gttcggtgcg
     4921 tccttgcccg tggtgaagat gggtcgcatg gcgggacaat ttgccaagcc acggtcgtcc
     4981 gacagcgaaa cccagggtgc cgtgacgctg cccagctatc gcggcgacat cgtcaatggc
     5041 cccgaattca cccccgaatc ccgcatcccc gatcccgctc ggatggagtt cggctatttc
     5101 cagtccgcga gcacgctgaa cctgctgcgg gcattctcgt ccggcggtta tgccgatctg
     5161 cacgaggtgc atcgctggaa tctcgacttc gtggaccgtt ccccgctggc cgagcgctac
     5221 cgcgacctgt cgtcgcgcat cgatgagacg ctgtccttca tggccgcctg cggcatgacc
     5281 agtgctacca cccgcgacct acgtgaaacc gacttctaca tcagtcacga ggcgctgctg
     5341 ctgccgtatg agcaggcact ggcccgcgtg gattcgacct ccggcgactg gtatggctgt
     5401 tcggcgcatt tcctgtggat aggcgatcgc accaggcagg ccgagggcgc ccatgtcgag
     5461 ttcctgcgcg ggctgcaaaa tccgctggga atgaaggtcg gcccgaccca ggaggcggat
     5521 gacctgctga agctgctgga catcctcaac ccgaagaacg aggcgggccg cattaccttg
     5581 attagccgca tgggcgccga caaggtcgag gcgaagctga cgccgctggt gcgcgcggtt
     5641 gaacgctccg gccacaaggt ggtctggctg tgtgacccga tgcacggcaa caccatcagc
     5701 acatcgtcga agatgaagac ccgcaacttc gactcgatcc ttcacgaagt ccgcagcttc
     5761 ttcgatatcc atgccgccga aggcacctgg gccggcggag tccacgtaga aatgaccggt
     5821 caggacgtca ctgaatgcat cggcggggcg caccggttgt ctgaagcgga ccttggcggc
     5881 cgctatgaga cgttctgcga cccacgcctc aacgccgaac agtccctgga actggcattc
     5941 ctggtggccg aggaactgaa gacgcgtcgc gcgcccgccg cggcgccggc aatcgccgcg
     6001 caatagggcc agcaggtggc ccaagccggt gaccacgagg ggcgggatct cgcggccgat
     6061 atcgccgcca ggaccgacag ctacttcaac cgcaccaaga cgatcgtggc gcggtttggc
     6121 gaccggcaag ttacctacgc agtgttcctg cgccggccgg tgatctgcgc gccccggttg
     6181 atgctggatt ggctgaagat ggttgccgaa ctaagagcta cggacatcga cgtcgatctg
     6241 atccaccaag aaggagattg ggtcggcgcc ggcgaaccga tcgcctatct cactggcagc
     6301 ctggtacatc tatcggatct ggaaacgatc ctgctgcaga aagtaggacc agtctgcgtc
     6361 gccgcgcata acgcatacca gatggcgctg tcat
//
LOCUS       DASWQN010000120         3492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_672860, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000120 DASWQN010000000
VERSION     DASWQN010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672860"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2129)
                     /locus_tag="VGC82_02005"
     CDS             complement(<1..2129)
                     /locus_tag="VGC82_02005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0422079.1"
                     /translation="MSQSLRVSRIHLAATVDIVLWRALAGARRISRHSPWLATLLRRL
                     VLLLWWTATSQLHVHARYWLRARRLRRVVPAPVPAPVIDIVDPSRLNVPLSDDPVVSV
                     IVPTYGQIQFTLRCLASIAEHKPVLPIEVIVVDDAWGGADAASLTLVRGIRLIRNETN
                     LGFLHRCNKAAALARGQYLYFLNNDPQVRGEWLDPMVSLFRQRPDTGAVGAKLLYPDG
                     RLQEAGGIVWQDASGWNFGRLEDPANPAYNYVREVDYCSGAALMVKRTVFDTLGGFDR
                     RYAPAYFEDTDLCFRLRRIGLKTLYQPAAQVVHHEGVSHGRDTAVGIKSYQVTNRRIF
                     VETWAEILAESHYPSGNNVFRARDRAMHRKVVLVVDHYVPTPDHDAGSRAIMAVLEVL
                     VEAGFVVKFWPHNRSYSAGYTETLQAMGIEVFHGPGDVAFATWIKMVGNELDTVLVSR
                     PDVAEDVIPLIRRHSKARVVYYGHDLHFQRMRLQAEVTSDSRLLLEAEHMREREYAIW
                     RQADLSLYLSDEEARIASELQPNARIGFVVPYCFDRFAAPRAAPAGQEIVFVAGFGHP
                     PNEDAASWFVEEILPLIRAEVPAAHLSIIGSNPTARVRALAANGIAVYANVNDAELAA
                     SYDRARVAVVPLRFGAGVKLKVVEALRDGVPLVTTGVGAQGLPGLSGVAAVEDAATSF
                     AAAVVALLRDDAEWEKCCHRQVMYAEER"
     gene            complement(2192..2977)
                     /locus_tag="VGC82_02010"
     CDS             complement(2192..2977)
                     /locus_tag="VGC82_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0422080.1"
                     /translation="MQHGTYEHIAVDVAGNIATIEVRRGPNNFIDTDMVAEIADALDI
                     YDRTPDVRAIVLCSEGKHFCAGADFGSRGPDGVSRAAKRGRHLYKEAQRLWRTGKPIV
                     AAVHGAAIGAGVGLAVMADFRVACPEARFSTNFTRLGFHPGFGLTATLPRLIGEQNAA
                     MMMYTGRRLTGQEALAIGLVDYCVPLDQVRIKAIEIATEIAGSAPLAIVATRETLRRG
                     LADAVARATEREYEEQDWLRNTADFKEGTKAMGERRPPNFQAR"
     gene            complement(3073..>3492)
                     /locus_tag="VGC82_02015"
     CDS             complement(3073..>3492)
                     /locus_tag="VGC82_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0422081.1"
                     /translation="SAGWYRYEKGDRTPHPDPEVAAIIKSVAAEMGVPQKVFTDTEIL
                     QRLLFASVNEACRILEEGKAYRASDIDVMWLHGFGFPRYRGGLMYWADGIGVRDVYNQ
                     IANWHQQYGERWAPSKLLRELAETNTPFRDAKPAPFA"
BASE COUNT          643 a         1139 c         1093 g          617 t
ORIGIN      
        1 cgttcctcgg catacatcac ttgtcggtgg caacattttt cccattcggc gtcgtcgcgc
       61 agcaaagcaa ccactgcagc ggcgaacgac gtggctgcgt cctccaccgc ggccaccccg
      121 gacaaccccg gcaggccttg cgcgccaacc ccagtggtga cgaggggaac gccatcgcgc
      181 agcgcttcca ccactttcag cttcacgccc gcgccgaaac gcagcggcac aacggccacg
      241 cgcgcacgat cataggaagc tgccagttca gcgtcgttga cattggcgta aacggcgatg
      301 ccgtttgccg ccagggcccg cacccgcgcg gtggggttgg agccgataat cgacagatgc
      361 gccgccggga cttcggcgcg gatcagcggc aggatttctt ccacgaacca cgatgcggcg
      421 tcctcgttcg gcggatggcc gaagcctgcc acgaacacga tctcctgtcc cgccggcgcg
      481 gcccgcggtg cagcaaaccg atcgaagcag taaggcacga cgaaaccgat cctggcattc
      541 ggctgaagtt cggatgcgat cctggcctcc tcgtccgaca gatacagcga caggtcggct
      601 tgtctccaga tcgcgtattc ccgttcgcgc atgtgttcgg cctcaagcag aagcctcgag
      661 tcgctggtaa cctcggcctg cagccgcatt cgctggaaat gcaggtcgtg gccgtaatac
      721 accacgcgcg ccttggaatg ccgccggatc aaggggatca cgtcctcggc gacgtcagga
      781 cggctgacca ggacggtgtc gagttcgttg cccaccatct tgatccatgt cgcgaacgcc
      841 acatcgccgg ggccgtggaa gacctcgatg cccatggcct gcagcgtttc ggtgtatccc
      901 gcgctgtaac ttctgttgtg cggccagaac ttcacgacga agcccgcttc gaccaggact
      961 tccaggaccg ccatgatcgc gcgcgaaccg gcatcatggt cgggcgttgg aacgtaatgg
     1021 tcaacgacca gaacgacctt gcggtgcatc gcccggtcgc gggcgcgaaa gacgttgttg
     1081 ccgctgggat agtgactctc tgccaggatt tccgcccatg tctcgacgaa aattcgccgg
     1141 ttcgtcacct ggtaggactt gatgcccacg gcggtatccc ggccgtgcga cacgccctca
     1201 tgatgcacca cctgagccgc cggctggtac aaggtcttca ggccgatccg ccgcaggcgg
     1261 aaacatagat cggtatcttc gaaataggcc ggtgcgtagc gtcggtcgaa gccgcccagg
     1321 gtgtcgaaca ccgtccgctt caccatcaac gcggcgccgg aacagtagtc cacttcccga
     1381 acatagttgt acgcagggtt tgcgggatcc tcgagacgac cgaaattcca gcccgacgca
     1441 tcctgccaga cgatgccgcc ggcttcctgc aagcgcccgt caggatagag cagcttggca
     1501 ccgaccgccc ccgtgtccgg ccgctgccgg aacagcgaca ccatcgggtc cagccattcg
     1561 ccccgcacct gcgggtcatt gttgaggaag taaagatact gtcctcgcgc cagcgctgcc
     1621 gccttgttgc aacggtgcag gaaccccagg ttcgtttcgt tccgtatcag ccggatgccc
     1681 cgcacgaggg taagggatgc cgcgtccgca ccgccccagg cgtcatcgac gactatcacc
     1741 tcgatcggca atacaggctt atgttcggcg atcgaggcca ggcagcgcag ggtaaactgg
     1801 atttgcccgt aggtgggaac gatcaccgag accaccggat cgtcggaaag cggcacgttc
     1861 aggcggctag gatcgacaat gtcgatgacg ggggcgggta ccggcgcggg aacgacacgg
     1921 cgcaggcgcc gcgcccgaag ccagtaccgg gcatgaacgt gaagctgtga tgtggcggtc
     1981 caccagagca gcaacaccag gcggcgaagc agcgtcgcga gccatggcga gtgccggctg
     2041 atgcgacgtg cgccagccag ggcacgccac agcacaatat cgaccgtcgc cgcgagatgg
     2101 atgcgtgaaa cgcggagtga ctggctcacc caaggcccct gtcctggagg ccccgtccta
     2161 gcggcggagt gttaacaagc ccttaaggtc gttaccgagc ctggaagttc ggcggccggc
     2221 gctcgcccat ggccttggtg ccttccttga aatcggcggt gttgcgcagc cagtcctgct
     2281 cttcgtactc acgctcggtc gcccgggcga cggcatcggc cagacctcgg cggagcgttt
     2341 cgcgcgtggc gacgatcgcc agcggcgccg atccggcgat ctcggtggcg atctctatgg
     2401 ctttgatccg cacctggtcc agtggcacgc agtaatcgac caggccgatc gccaaggctt
     2461 cctgcccggt caggcgtcgt ccggtgtaca tcatcatcgc cgcgttctgc tcgccgatca
     2521 ggcgcggcag cgtggcggtc aggccaaagc cgggatgaaa acccaggcgg gtgaagttgg
     2581 ttgaaaagcg cgcttccggg caggcaacac ggaaatccgc catcaccgcg agaccgacgc
     2641 cggccccgat cgcggcgcca tgcacagcgg cgacaatggg tttgccggtg cgccacaacc
     2701 gctgcgcttc cttgtagaga tgccggccgc gcttggccgc gcgggaaacc ccgtcggggc
     2761 ctcggctgcc aaaatccgcg ccggcgcaga aatgcttgcc ttcagagcaa aggacgatcg
     2821 cgcggacatc cggtgtgcgg tcgtagatgt ccagcgcatc ggcgatttcc gccaccatgt
     2881 cggtgtcgat gaagttattc gggccgcgcc gcacctcgat cgtcgcgatg ttgccggcca
     2941 catcgaccgc gatgtgctca taggtgccgt gctgcatggt gatttcctgc cccgttgcgt
     3001 taggaatgga agtcttaggt gagtggggtg acagcggcaa tccgacacgg ccggcgttgc
     3061 cgaacggcac cttcaagcga atggtgccgg tttcgcgtca cggaacggcg tgttggtttc
     3121 ggctagttcc cgcaacagct tcgatggcgc ccagcgctcg ccgtactgct gatgccagtt
     3181 cgcgatctga ttatagacgt cgcggacgcc gatcccgtcc gcccagtaca tcaggccgcc
     3241 gcgatacctc gggaacccga acccgtgcag ccacatcaca tcgatgtcgc tggctcggta
     3301 ggcttttcct tcttccagga tacggcaagc ctcgttgacg gaggcgaaca gcaagcgctg
     3361 caggatttcg gtgtcggtga aaaccttctg cggcacgccc atctcggctg cgaccgattt
     3421 gatgatcgcg gcgacttccg gatcgggatg cggcgtgcga tcacccttct cgtaacgata
     3481 ccatccggcg ct
//
LOCUS       DASWQN010000121         3504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2520443, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000121 DASWQN010000000
VERSION     DASWQN010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2520443"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VGC82_02020"
     CDS             <1..324
                     /locus_tag="VGC82_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422082.1"
                     /translation="GGWNIFHTWHAGADCVNPAPYIALNASGDTAWFGWPKSDLVQEK
                     IAAWYDAPDLAAEKAVIADLNKAAMDHVIYVPTGFFKGYQAWRKNLSGVVKAPFPVFW
                     DVVKT"
     gene            489..1430
                     /locus_tag="VGC82_02025"
     CDS             489..1430
                     /locus_tag="VGC82_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0422083.1"
                     /translation="MLSYIARRIAATIPVVLMVALFVFSLLYIAPGDPAAIIAGDQAT
                     PADVDRIRASLGLDRPFLIRFFDWFWHVLQGDLGTSIFTNLPVTHMIAQRIEPTLSLM
                     TLTLILSLSCAIPMGVIAAWKHGTWIDRTVMMVAVLGFSTPVFVIGYVLAYVFALRLD
                     WLPVQGFTSISVGVLPFLRNLILPAVALGLVYMALIARITRATMLDVLAQDYVRTAKA
                     KGVGQHDVLFIHALKNAAVPIITIIGIGFAALIGGAVVTESVFAIPGLGRLTVDAILR
                     RDYPVIQGVVLLFSFVYVLVNLGVDLLYTVFDPRIRY"
     gene            1496..2359
                     /locus_tag="VGC82_02030"
     CDS             1496..2359
                     /locus_tag="VGC82_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0422084.1"
                     /translation="MPGILPVPKERGRIGGFIYRNPTIAVGGLLVGLMILIAILAPYL
                     GTKDPTSLAPARRTREPSALYWFGTDMLGRDVYSRVLYGARVSLMVGFSVAFCASILG
                     TFIGLVAGFIRQIDAVVMRVMDGLMSIPPILLAIALMALTRASVQNVIVAISIAEFPR
                     VSRLVRGVVLSLREQPYVEAAVASGTRVPVIIWKHILPNTLAPLMVQATFICASAMIT
                     EATLSFIGAGTPPIIPSWGNIMAEGRALWQVKPYIVFFPAIFLSITVLAVNLLGDGLR
                     DALDPRLAKRL"
     gene            2363..3352
                     /locus_tag="VGC82_02035"
     CDS             2363..3352
                     /locus_tag="VGC82_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0422085.1"
                     /translation="MALLEVEKLQTHFGTMDGVVRAVEGLSFHIDAGETLGIVGESGC
                     GKSVTSMSILRLIQEPPGKIAGSIRFEGRDLLKVSEQEMRAIRGNAISMIFQEPMTSL
                     NPVLTVGQQIGETVQLHQGMNSRDAEAKAVEMLTLVGIPAPGRRVREYPHQLSGGMRQ
                     RVMIAMALACNPRLLIADEPTTALDVTIQAQILDLMRDLKTRLGSAIMLITHDLGVVA
                     EMAQRVVVMYAGRKVEEAPVNEIFARPMHPYTRGLLGAVPKLGSSLQDGRRTRLAEIG
                     GLVPSLRKPIVGCAFAGRCPMVTDFCRTVAPAIEPKAAGHSVACHYAGHKVAA"
BASE COUNT          580 a         1079 c         1168 g          677 t
ORIGIN      
        1 ggcggctgga acattttcca cacatggcac gcgggcgcgg attgcgtgaa cccggcgcct
       61 tacatcgctt tgaacgcaag cggcgatacc gcctggtttg gctggccgaa gtcggatctg
      121 gtgcaggaaa agatcgccgc ctggtacgac gcacccgatc tggcggcgga gaaagcggta
      181 atcgccgatc tgaacaaagc ggcgatggac catgtgatct atgtgcccac cggattcttc
      241 aagggatacc aagcctggcg gaaaaacctg tcgggcgtgg tgaaggcgcc gttcccggtg
      301 ttctgggacg tggtcaagac atgagcggga gttgtggcgg gacgaagtcg tgggtggccg
      361 gccaggcccg gccatgacac atgtggcgca acggcaagac acaggcatgg cacggatagc
      421 gcagcggcgt gacaagggtg gcgaaaaggc atgaggcgca gatgaccccg agcaggaccc
      481 cccgctagat gttgtcctat atcgcccgcc gcatcgcggc cacgataccc gtggtgctga
      541 tggtggcact gttcgtcttc agtcttctct acatcgcgcc cggcgatccg gcggcgatca
      601 tcgccggcga ccaggccaca ccggcggatg tcgaccgcat ccgggcatcg ctcggtctgg
      661 accggccatt cctgatacgc ttcttcgatt ggttctggca tgtcctgcag ggcgacctgg
      721 gcacctcgat cttcaccaac ctgccggtca cccacatgat cgcgcagcgg atcgagccaa
      781 cgctttcgct gatgacgctg acccttatcc tgtcgctgag ctgtgccatt ccaatgggcg
      841 tgatcgccgc atggaagcac ggaacctgga tcgaccggac cgtgatgatg gtggcggtgc
      901 tgggcttctc caccccggtt ttcgtcatcg gctacgtgct ggcctacgtg ttcgcactgc
      961 ggctggattg gctgccggtg cagggcttca cctcgatcag cgtcggcgtg ctgccgttcc
     1021 tgcgcaacct gatcctgccc gccgtcgcgc ttggactggt gtatatggca ctgatcgcgc
     1081 gcatcacccg ggcgacgatg ctggatgtgc tggcacagga ttacgtgcgg accgccaagg
     1141 cgaagggcgt cggccagcac gacgtgctgt tcatccacgc gctgaagaac gcggcggtcc
     1201 cgattatcac catcatcggc atcggtttcg cggcgttgat cggcggcgcg gtggtgaccg
     1261 agagcgtgtt cgccattccc ggcctcggcc ggctgaccgt cgatgccatc ctgcggcgcg
     1321 actatccggt cattcagggc gtggtactgc tgttcagctt cgtctatgtg ctggtcaacc
     1381 tcggcgttga cctcctttac accgtgttcg acccgaggat ccgctattga gcgccaccac
     1441 ccccgaattc gccacccccg aattcgccac ctccgacttc accgccgctc cggacctgcc
     1501 aggcatcctg ccggtgccga aggagcgtgg tcgcatcggc ggcttcatct accggaatcc
     1561 gacgatcgct gtcggtggcc tgctggtcgg cctgatgatc cttattgcga tcctggcgcc
     1621 ctacctgggc accaaggacc ccacctcgct ggcgccggcc aggcggaccc gggaaccttc
     1681 ggcactctac tggttcggca ccgacatgct cggccgggac gtttattccc gtgtgctcta
     1741 tggcgcgcgc gtatcgctga tggtcggatt ctcggtggcc ttctgtgcct cgatcctggg
     1801 cacgttcatc ggcctggtcg ccggtttcat ccggcagatc gacgcggtgg tgatgcgggt
     1861 catggacggc ttgatgtcga tcccgcccat cctgctggcg atcgcactga tggcgctgac
     1921 gcgggcgtcg gtgcagaacg tcatcgtcgc catcagcatc gccgaatttc cccgggtttc
     1981 gcggctggtg cgcggcgtgg tgctgtcgct gcgtgagcaa ccctatgtcg aggccgcggt
     2041 cgcttccggc acgcgggtgc cggtcatcat ctggaagcac atcctgccca acacgctggc
     2101 gcccttgatg gtgcaggcga cgttcatctg cgcctccgcc atgatcaccg aggcaacatt
     2161 gtccttcatc ggcgcgggca cgccaccgat catcccgagc tgggggaaca tcatggccga
     2221 agggcgggcg ctgtggcagg tgaagccata catcgtgttc ttcccggcga ttttcctgtc
     2281 gatcacggtg ctggcggtca acctgctggg tgacgggctg cgggacgcgc tcgatcctcg
     2341 tttggcgaag cggttgtaat cgatggcgct gctggaggtc gaaaaactgc agacccattt
     2401 tggcaccatg gacggcgtcg tccgggctgt cgaaggcttg tcctttcaca tcgacgcagg
     2461 cgaaacgctg ggcatcgtcg gcgagtccgg ctgcggcaag tcggtgacct cgatgtcgat
     2521 cctgcggctg atccaggagc ctcccggcaa gatcgccggc agcatccgct tcgaaggacg
     2581 cgacctgctg aaagtcagcg aacaggaaat gcgggcgatc cgcggcaatg cgatctcgat
     2641 gattttccag gagccgatga ccagcctgaa cccggtgctg acggtcgggc agcagatcgg
     2701 cgaaacggtg cagctgcacc aggggatgaa ttcccgcgat gccgaggcga aggcggtcga
     2761 gatgctgacg ctggtcggga ttccggcgcc ggggcggcgt gtgcgggagt atccgcacca
     2821 attgtccggt ggcatgcgcc agcgtgtcat gatcgcgatg gcgctggcct gcaatcccag
     2881 gctgctgatc gccgacgagc cgaccacggc gctggatgtg acgatccagg cgcagatcct
     2941 ggacctgatg cgcgatctga aaacccggct ggggtcggcg atcatgctga tcacgcacga
     3001 ccttggcgtg gtcgcggaaa tggcgcagcg ggtcgtggtc atgtacgccg ggcgcaaggt
     3061 cgaggaggcg cccgtcaacg aaatcttcgc ccgcccgatg catccctata cacggggcct
     3121 gctgggcgcg gtgccgaaac tcggctcatc gctgcaagat ggcaggcgca cgagattggc
     3181 ggagatcggg gggctggtgc cgagcctgcg gaagccgatc gtggggtgtg cgttcgccgg
     3241 acgatgcccg atggtgaccg atttttgccg cactgtggcg ccggcgatcg agccgaaggc
     3301 ggccggtcac agcgttgcct gccactatgc cggacacaag gtggcggcat gagtgccggc
     3361 cgcgccctgc tcgaagtaaa tgcgctcaag aaacactttc cgatccatgg tgggttgctg
     3421 ggccaggtga ctgccaaggt ctatgccgtc gatggcgtaa cgttcgatat caggcgcggc
     3481 gaaacgttgt cgctggtggg ggag
//
LOCUS       DASWQN010000122         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1464692, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000122 DASWQN010000000
VERSION     DASWQN010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464692"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1684
                     /locus_tag="VGC82_02040"
     CDS             197..1684
                     /locus_tag="VGC82_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0422086.1"
                     /translation="MPAKPIVRSTAVSRQTRLTALIVASALFMQNLDSTVIATALPTM
                     ARSFGYDPVRMNVALTSYLLSLTVFIPASGWVADRFGTRTVFRLAIVVFTIGSILCGL
                     SDGLVELVASRVLQGIGGAMMVPVGRLLLLRTAEKSELVAAMAWLTVPALLGPVVGPP
                     VGGFIVTYFNWRWTFFINIPVGLIGLVAVTLFIQDFREPPRGKFDIRGLVLTGLALVC
                     GMFGLETVGRGLVSPEVTGAMIAAGLAMALLYYLHARRTAAPLLDFTLMRLPCFGLSF
                     SAMMLFRTGIGAIPFLLPMMLQIGFGNSAAQSGLITFASSAGALVMKPAAQHALRLFG
                     FRDTLIWNGVLSGIMLTMCALFRPTWPAAAIYLVLLVGGFLRSLQFTAYNTLAYGDVP
                     RPQMSAATSLYTAGQQLAATIGVSIGALALEVARTLDHHDTPEAADFSAAFVVVGLMT
                     LAAVPIAFLMPASAGDDLTGRHTPAPLSPTSSSSSPSPPMPRAGE"
     gene            complement(1685..2596)
                     /locus_tag="VGC82_02045"
     CDS             complement(1685..2596)
                     /locus_tag="VGC82_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY0422087.1"
                     /translation="MTRRFYTLDVFTGQVLAGNPLAVVLDAQGLDGRRMQAIAREFNL
                     SETVFVAAPADPAHRAGIRIFTPGREVPFAGHPTVGTAVLLGLLDRRGQVGRVELVLH
                     EKIGPVSCQVEVLGSEHGHASFSLPRLPERIGTLPDNAILARGLGLEPEDIGFGAHEP
                     AVYSAGNPFRCVPLRSRDAVTRARPQGEAFVRAFDGLADGFTNAYVYCAEPLDPMHAF
                     HVRMFAPSVGVPEDPATGSAAAAFAGVFMDFEKPGDGQHTFVIEQGDAMGRPSRITVS
                     LDVAGGMLRDARVGGEAVIVSDGRLRE"
     gene            2818..3030
                     /locus_tag="VGC82_02050"
     CDS             2818..3030
                     /locus_tag="VGC82_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422088.1"
                     /translation="MRLNSAHRYEELAEHIITLIDNGTLANSNCLRRSCGQTWDERMQ
                     TGLRIPGAWASRAIAELDGHPKPKRR"
BASE COUNT          513 a         1061 c          968 g          591 t
ORIGIN      
        1 cggcgctgtc ccttcctggc ctcggcgaga tgctcaaaaa tccgccgtta cgccgggacg
       61 cctgggcggg cttgcgcttg ctgcgtcgca ccatcgatgg gactcccccc ttgccagtcc
      121 aacagccata aacgcacggg atcgacgatt ggccttgggg cttccccgcg gcctcctgcc
      181 gcgcgacact ggcggaatgc cggccaaacc catcgtccgt tcgaccgcgg tttctcgtca
      241 aacccgcctg accgcgctga tcgtcgcttc tgcgctgttc atgcagaacc tcgactcgac
      301 ggtgatagcg accgccctgc ccaccatggc gcggtcgttc ggctatgacc cggtgcggat
      361 gaacgtggcg ctgacatcgt acctgctgag tctgactgtg ttcattccgg ccagcggctg
      421 ggtggccgac cgcttcggca cgcggaccgt gttccgactg gcgatcgtgg tgttcaccat
      481 cgggtccatc ctgtgcggcc tgtccgacgg cctggtggag ctggtcgcgt cacgcgtcct
      541 gcaaggcatc ggtggggcga tgatggtgcc ggtcggccgc ctgctgctgt tgcggaccgc
      601 cgagaagtcg gagcttgtcg ccgccatggc ttggctgacg gtgcccgccc tgctggggcc
      661 ggtggtcggt ccgccggtcg gcggcttcat cgtcacctac ttcaactggc gctggacgtt
      721 cttcatcaac atcccggtcg gcctgatcgg ccttgtcgct gttacgctct tcatccagga
      781 ctttcgcgaa ccgccccggg gcaaattcga tattcgtggc ttagttctga cagggctggc
      841 gctggtgtgc ggcatgttcg ggctggagac ggtcggtcgt ggcctggtct cccccgaggt
      901 gaccggcgca atgatcgccg ccggcctggc catggccttg ctttactatc tgcacgcacg
      961 ccgaacggcc gctccgctgc tggatttcac attgatgagg ctgccgtgtt tcgggttgtc
     1021 gttcagcgcc atgatgctgt tccgcaccgg cattggcgcg atcccattcc tgctgccgat
     1081 gatgttgcag atcggtttcg gcaattccgc ggcgcagagc ggcctgatca cgttcgccag
     1141 ttccgccggc gcattggtga tgaagcccgc ggcgcaacat gccctgcggc tgttcgggtt
     1201 ccgcgacacg ctgatctgga atggcgtgtt gtccggcatc atgctgacca tgtgtgcctt
     1261 gttccgcccg acctggccgg ccgccgcgat ttacctggtc ctgctggtcg gcggcttcct
     1321 gcgctcgttg caattcactg cctataatac cctggcctac ggcgacgtgc cccgcccgca
     1381 aatgagcgcc gcgaccagcc tttataccgc cggtcagcag cttgccgcga ccattggggt
     1441 ttccatcggc gcattggcgc tggaagtcgc ccgcacgttg gaccaccacg acacgccgga
     1501 ggcggccgac ttcagcgccg ccttcgtcgt ggtcgggctg atgacactgg cagccgtgcc
     1561 gatcgcgttc ctgatgccgg ccagcgccgg tgacgacctg accggacggc atacccctgc
     1621 cccgttgtcc cccacatctt cgtcctcctc accttcgccc cccatgcccc gggccggcga
     1681 gtagctattc cctcagccgg ccatcgctga cgatcaccgc ttcaccgccg actctcgcgt
     1741 cccgcaacat gccccccgca acatcgagcg acaccgtaat gcgggacgga cggcccatcg
     1801 catcgccctg ctcgatcaca aatgtatgct ggccgtcgcc gggcttttcg aagtccatga
     1861 acacgccggc gaaggcggca gccgcggaac cggtcgccgg atcctccgga acgccaacgc
     1921 ttggtgcgaa catccgcaca tgaaaggcgt gcatgggatc gagcggttcg gcgcaataga
     1981 cataggcatt ggtaaaacca tccgccaacc catcgaacgc ccgcacgaac gcttccccct
     2041 gcgggcgcgc ccgggtcacc gcgtcccggc tgcgcaacgg cacgcaccga aacggattgc
     2101 cggcagagta aacggcgggc tcgtgtgcac cgaagccgat atcttccggc tccagaccca
     2161 ggccgcgtgc caggatcgcg ttgtccggca atgtcccgat ccgttccggc aggcgcggca
     2221 ggctgaacga agcgtggccg tgttcggatc ccagcacctc gacctggcat gacaccggcc
     2281 cgatcttctc gtgcagtacc agttcgaccc gcccaacctg acctcgccgg tccagcaagc
     2341 cgagaagcac ggcggttccc acggtcgggt gcccggcgaa cgggacttcg cgaccggggg
     2401 tgaaaatgcg gatgcccgct cggtgtgcgg gatcagccgg agcggcgacg aacacggtct
     2461 ccgaaaggtt gaactcccgc gcgatggcct gcatccgccg gccgtcgagg ccttgcgcat
     2521 ccagcaccac ggccagcggg tttcccgcca gcacctgtcc ggtgaagaca tccagcgtgt
     2581 agaagcgtcg ggtcatgctt gcagtgcctt agggttgctg cacgttcgaa ggatccaggc
     2641 atatccgcgg ctaacggttc gcgacggctt cagcgcgcac ggtccaggcc ggtccgattt
     2701 gatgcacgcc atgcacaatg ctgtatccgg tccgtcggca ccataacaga tacaggttcc
     2761 tggaattgtg cacagcacag tggtagcgac ggcggacggc cgccaaaggc gaagttcatg
     2821 cggctgaatt cagctcaccg ttatgaagaa ctggcagagc acatcataac gctgatcgac
     2881 aacggcaccc tggcgaacag caactgcctg cgacgtagtt gcggccagac ctgggacgaa
     2941 cggatgcaga ccgggctgcg aatcccgggc gcatgggcga gcagggcaat tgccgaactg
     3001 gacggccatc ctaaaccgaa gcgccggtaa tccaagcgta atgcaggttc attagtggcc
     3061 aacatcgaca acttcactga cccggttacc gaccgtgcag gttcccggcg cgatgtctct
     3121 cgcgaaggtt tcg
//
LOCUS       DASWQN010000123         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_936813, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000123 DASWQN010000000
VERSION     DASWQN010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936813"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VGC82_02055"
     CDS             complement(<1..599)
                     /locus_tag="VGC82_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0422089.1"
                     /translation="MTTAAMTKVQESAGAPSGLPDMKVSVRQVFGIDSDLEVPAYSEV
                     DPHVPDVDTDYRFDRATTLAILAGFARNRRVMVTGYHGTGKSTHIEQVAARLNWPCVR
                     VNLDSHISRIDLVGKDSIVVRDGKQVTEFRDGILPWALQHNIALVFDEYDAGRPDVMF
                     VIQRVLEVSGRLTLLDQNKVIKPHPSFRLFSTANTVGLG"
     gene            907..>1544
                     /locus_tag="VGC82_02060"
     CDS             907..>1544
                     /locus_tag="VGC82_02060"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY0422090.1"
                     /translation="MASFLDPLIAFVSANAWLAYLTLFLAALLEAVPVAGSVIPGSTI
                     ILALSGLIPGGELKLEWVMAAAIAGALLGDGAAFWIGHRAQREMLKAWPLKNYPGLVA
                     QSEAFFHRWGALAVFFARFVAPIRAVVPITAGALDMPPRHFYPVNFVAILLWAPAHIL
                     PGIVAVSALHRYGGIAHDTGIGRHYWILAVIGGAVMIALASWIIRRRRHGAA"
BASE COUNT          248 a          465 c          514 g          317 t
ORIGIN      
        1 tcgccgaggc ccaccgtgtt ggcggtcgag aacagccgga acgacggatg cggcttgatc
       61 accttgttct gatccagcag cgtcaggcgg ccggagactt ccagcacgcg ctggatcacg
      121 aacatcacgt cgggacggcc ggcgtcgtat tcgtcgaaca ccagcgcgat attgtgctgc
      181 agcgcccagg gcaggatgcc gtcgcggaat tccgtgacct gcttgccgtc cctgacgacg
      241 atggagtcct tgccgaccag atcgatgcgg ctgatgtggc tgtcgagatt gacccgcacg
      301 cacggccagt tcagccgcgc ggcgacctgc tcgatatggg tcgatttgcc ggtgccgtgg
      361 tagcccgtga ccatgacgcg gcggtttctt gcaaagccgg ccaggatggc gagcgtggtg
      421 gcgcggtcga agcggtaatc ggtatcgaca tcaggcacat gcgggtcgac ttcggaatag
      481 gccggcacct cgaggtcgct atcgataccg aatacctgac gcaccgacac tttcatgtcg
      541 ggcaaaccgg agggcgctcc ggcagattcc tgcactttgg tcatggcggc ggtcgtcatc
      601 aatcctccga ggtcccggat gctcccgaaa ccagatttgc ggcatggctg cggatgggtg
      661 ctcacggaac ctatcagaga gccacggctg gatgaagccc gccgcgcaat caaagttccg
      721 tcgctatttc atcaagatag gcgtggggtc cggtttttgg aaggctgccc tgcgaatcac
      781 gccgcacttg ccgcccaacg gggccgcctc tcccaaggcg ggcctggccc ggatggcact
      841 ttccatcctg ctgactaggt atgtcaggtc ggcgcccttc ccagacaatg ccgcgacagg
      901 atttttgtgg cttcctttct cgatcccttg attgcgttcg tttcggcaaa tgcgtggctt
      961 gcctatctga cgctgttcct tgccgccttg ctggaggccg ttccggtggc gggttcggtg
     1021 atcccgggct cgaccatcat cctggcgctg agcgggctga tccccggtgg agagttgaag
     1081 ctggaatggg tgatggcggc ggcgatcgcc ggtgcgttgc tgggcgacgg agcggcgttc
     1141 tggatcggac atcgcgccca gcgcgagatg ctcaaggcat ggccgctgaa aaattatccc
     1201 ggactggtgg cgcaaagcga ggcgttcttt catcgctggg gcgcgctggc ggtgttcttt
     1261 gcccgtttcg tggcgccgat cagggcggtg gtaccgatca cggcgggcgc gctggacatg
     1321 ccgccgcggc acttctaccc cgtcaatttc gtggcgatcc tgctatgggc accggcccat
     1381 atactgccag gcatcgtggc ggtatcggcg ctgcatcgct atggcggcat tgcccacgat
     1441 accggcatcg gcaggcatta ctggatcctg gcggtgatcg gcggcgctgt gatgattgcg
     1501 ctggcgagct ggatcattcg ccgccggcgc catggcgccg cgat
//
LOCUS       DASWQN010000124         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2520494, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000124 DASWQN010000000
VERSION     DASWQN010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2520494"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VGC82_02065"
     CDS             complement(<1..320)
                     /locus_tag="VGC82_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEY0422091.1"
                     /translation="MEPHSHVFLGKGHEHAEYRTWAVIWLCGAMMAVEVSGGWLFGSI
                     ALVADGMHMSTHAGALLLAALAYRYARRHAADTRFTFGTGKLGDLAGFTSAIVLAMIA
                     LLIA"
     gene            559..1182
                     /locus_tag="VGC82_02070"
     CDS             559..1182
                     /locus_tag="VGC82_02070"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0422092.1"
                     /translation="MEQLPGLDQAAFDKLDRAPDAAFYAQPRFVTHIDDAAIAVVTQA
                     YRELLPANGAVLDVMSSWVSHFPAECEYAAIGHGMNAEELAANPRLSRWFVQDLNIDP
                     ALPLGDDAFDAACLCVSVQYLQRPVEVFREVARVLRPNGRFIVSFSNRCFPTKAVAIW
                     QEFSGPDQQHLVAAYMRAAGFANVATRTSIPAWGDPVWLVIGETGGA"
     gene            1389..2243
                     /locus_tag="VGC82_02075"
     CDS             1389..2243
                     /locus_tag="VGC82_02075"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0422093.1"
                     /translation="MNTVVQTAYDAGHAITQLDLWLFHSINQWSGNFGPFDRLVWFQD
                     NIFKSGLLMGAYWWFWFVPEAQRERRHEIIWTFLGTIIALVAARALALLLPLRVRPMY
                     TDGIDYHSPLVLDPAVTHLNMETWSSFPSDQAALFCALAFGLWRLCRPVGFVALAVSG
                     IWVCLARVYLGIHFPSDVLVGAFIGIGSVYLAGRLGSHVLSAPVLNFERSFPQAFYPA
                     MFLVTYEFGILFDDLRHMMHGLMRAMRGLGLASANLEEALAALVGLGAIAALTLVFSI
                     RWLGKRRR"
BASE COUNT          422 a          719 c          738 g          516 t
ORIGIN      
        1 cacgcgatta ggagtgcgat catcgccagt acgatcgcgc tcgtgaatcc ggccagatcg
       61 cccagcttgc cggtaccgaa ggtgaaccga gtatcggcgg cgtgccggcg ggcatagcga
      121 taggctaaag ccgccagcag caatgctccg gcgtgggtgc tcatgtgcat cccgtcggcg
      181 accagcgcga tcgatccgaa cagccatcca ccggagactt caaccgccat catcgccccg
      241 cagagccaga tgacagccca ggtgcgatat tcggcgtgtt cgtggccttt gccgaggaaa
      301 acgtgactat gcggttccat gcctacctct tcctatagtg tccaccggat acctcgggcg
      361 gcttcgatgc atcgcccctg ctgaattgag cagaaggggg cggcgaggtt cgccaccacc
      421 tcgggcgtcc aatcctgcta tggcacaacg cctgaagccg acgactgtcc ggcgaataat
      481 ggcgtgacga tgacacgtcc aggtcaacag gaactcaagg tgattggcat gcggccgcga
      541 agcgacgata caccccgtat ggaacaatta ccgggccttg atcaggcggc gttcgacaaa
      601 ctcgatcggg caccggacgc ggcgttttac gctcaaccgc gtttcgtcac ccacatcgat
      661 gacgcggcga tcgcggtggt gacacaagcg tatcgcgagt tgctgccggc gaacggcgcg
      721 gtgctcgatg tcatgagcag ttgggtaagc cacttcccgg cggagtgcga gtacgcagcc
      781 atcggccacg gcatgaacgc tgaggagctg gcggcgaacc cgcgactctc gcgctggttc
      841 gtccaagacc tgaacatcga cccggcgctg ccgctcggtg acgatgcgtt cgacgcggca
      901 tgcctgtgtg tatcggtgca gtatttgcaa agaccggtgg aggtattccg cgaggtcgct
      961 cgcgtgctgc ggccgaacgg gcgcttcatc gtgtcgttct ccaaccggtg cttcccaacc
     1021 aaggcagttg ctatctggca agaattttcg gggccggacc agcagcatct ggtcgcggcc
     1081 tacatgcggg ctgccggctt cgccaatgta gccacgcgga cttcaatacc ggcttggggc
     1141 gatcccgttt ggctggtgat tggcgaaacc ggcggcgcgt gaccgtaggt tgcctcgata
     1201 tggatgtggt gaaataaacc acggcgctga aactatcggc cgtatttccg actcgccttc
     1261 agcggctcga cattcattcg gataacggcc tgcgacggcc ggccgaatcc cgttgtgcgg
     1321 cgcgctcgtg ccagagtaac gtcgcgggat tgaggtaacg acagtcagac cgggaaccga
     1381 acagcctgat gaatacggtt gtgcaaactg cttatgatgc aggccatgca attacccagc
     1441 ttgacttgtg gttgttccat tcgatcaatc aatggagcgg gaacttcggc ccgttcgatc
     1501 gtttggtctg gttccaggac aacatcttta aaagcggcct gcttatgggc gcctactggt
     1561 ggttctggtt cgtgccggag gcgcagaggg aacgcaggca cgagataatc tggacgtttc
     1621 tcgggaccat catcgctctg gtcgccgccc gcgcgcttgc tctattgctg cctcttcgtg
     1681 ttcgtccaat gtacaccgac ggcattgatt accattcgcc gctggtactg gacccggccg
     1741 tcacgcattt gaacatggag acctggagct cgttcccgag cgatcaggca gccctgtttt
     1801 gtgccctggc cttcggactt tggcggctgt gccgcccggt cggtttcgtc gccctcgccg
     1861 tctccgggat ttgggtatgt ttggcccggg tttatctcgg gatccacttt ccttcggatg
     1921 tgttggtcgg ggcgtttatc gggatcggca gcgtttatct cgccggccgc ctgggcagcc
     1981 atgtcctgtc cgcgccggtc ctgaacttcg aaaggagttt tccgcaggcc ttttatcccg
     2041 caatgttctt ggttacttac gaattcggca tattgttcga cgacctgcgc cacatgatgc
     2101 atgggttgat gcgagcgatg cgtggcctgg ggctggcttc cgcgaacctg gaggaagcgc
     2161 tggccgcgct tgtcgggctg ggcgctatag ctgccctgac gctggttttt tcgatccggt
     2221 ggctgggaaa gcgaaggcgc tgacgcaagc ctgcgcccaa tcgtgctgcc agggcaatct
     2281 cagcccgacc ctaccggccg ccaatgctct tccgctttcg tctgtcgcgt gatcgacatg
     2341 cttccaacgt tccgcccagc gctcccgcgg gtaacccgct acggcgcttc atagc
//
LOCUS       DASWQN010000125         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_13866, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000125 DASWQN010000000
VERSION     DASWQN010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13866"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..632
                     /locus_tag="VGC82_02080"
     CDS             420..632
                     /locus_tag="VGC82_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422094.1"
                     /translation="MLRLPSRFAAVILTFAPMFVQQRTWWHAQLLLLGAILTPGQRTV
                     SQLVAGFDVNRGGGHIDIEMTNRSSA"
     gene            complement(744..1214)
                     /locus_tag="VGC82_02085"
                     /pseudo
     CDS             complement(744..1214)
                     /locus_tag="VGC82_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1142..1651)
                     /locus_tag="VGC82_02090"
     CDS             complement(1142..1651)
                     /locus_tag="VGC82_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0422095.1"
                     /translation="MMPNRVGSNLLEDQMAKTQCWQTIPTQLALTEFEQFVLPHLVRG
                     SRGPAPKLSLYKVFNYVLKMLYLGCQWKELPIDKDDRGKPEVHYTRIYRSWRRWVDMG
                     CMDAIFAGSVAKLHEDGRLDPTVIHGDATTTAAKKGATTSASAGTRRSRATRSSPSAT
                     VTAMSSPHS"
BASE COUNT          378 a          564 c          603 g          380 t
ORIGIN      
        1 tcggcccccg ccgtgtgccg cgggctgaaa ccagtcggaa agccggatgc gggaaatccg
       61 cacgtccggt ttgatgagcg gggacgggaa acggagcgtt gccgcatggc ccaagccacc
      121 gcgcccgtcc tcgactctac gcgcgccccg acggcgcgta tcaccagtgg aggagggttc
      181 cacccggaga gttgtcgatt gctccggtag ctgacgagcg gcttggtcgg gtaactgacc
      241 gggccggagc ctcgtgacaa aggccgcctt gggcggctga gcaatccagc gggccgtaac
      301 gcgcgtgaag cctgagttcg actttggcca tttggcctcc cccgctgagc ggtggggtat
      361 gttgggtagc ggagcggaag gaggcataga ctggggtatc cctgccagga gacgccacca
      421 tgcttcgcct gccgtcccgc ttcgccgccg tcattctaac atttgcaccg atgtttgtgc
      481 agcagcgcac ttggtggcac gcgcaattac tgttgctggg tgcgatcctg acaccgggcc
      541 agcgcacggt cagccaactg gttgcgggtt tcgatgtgaa tcgaggcgga ggacatattg
      601 acatagaaat gacgaacagg agtagtgcgt gattcgtgac atagcgcaac gctattcgac
      661 gttttgggtc gcttttgagc gtgaaccgga caagcgcgat agcgccggaa tgaaatcctc
      721 cgcctggccg tcagcttttt agatcagctc tggcaatagt gccgcagatt gatcatcgtg
      781 tagccaaggg tcttgaaggc atagtgcagg ccgctgatgc gttcgaaacg cagcaacagc
      841 cggcggaact tgtcttccca ggcaaacact cgttcgatgg tcctgaagcg ctctgcgaaa
      901 atggatggct cgaagatcac cttgcgtccc cgcttcggct gctttcgacc gcgcggattg
      961 gggttgatgt tgggaatcat accgcgattg aagatcgcct tgcggttggc ccggcagtcg
     1021 tagacgccat cgagactgac gatgctgcct tgaaggtcca tgccgaccgc ccaggcgatg
     1081 cgcgtcacgt cggacagcgc ttcacgcaga agcggcgatt cgttgcggtt gcccggcgcc
     1141 gttacgaatg gggcgatgac attgcagtga cggtcgcaga aggcgacgac cttgtcgccc
     1201 ttgaccttct tgtgcccgct gaagccgagg ttgtcgcccc ctttttcgcc gccgtcgtcg
     1261 tggcgtcgcc atgaatgacc gtgggatcga ggcggccgtc ctcatgcagc ttggcgaccg
     1321 aaccggcgaa aatggcgtcc atgcagccca tgtccaccca cctccgccag ctacggtaaa
     1381 tgcgtgtata gtggacttcc ggcttgccac ggtcatcctt gtcgatcggc aactccttcc
     1441 actggcagcc caaatatagc atcttcagaa cgtagttgaa taccttgtaa aggctcagct
     1501 tcggtgcagg accgcgactc ccgcggacga ggtggggcaa gacaaattgc tcgaattccg
     1561 tcagagccaa ttgcgtcgga atggtttgcc agcactgggt tttagccatt tggtcctcca
     1621 aaagattgga ccctacccgg ttcggcatca tcggcataaa tcccaggacc aaggccgctt
     1681 acggccaatg ccgaccgccg ccagaaacca gcaaccagtt gcatgcgttc aggccggcct
     1741 cgacagggtg gcggatcagc tgaagtgagc gaaccggcga atagccggac gaggccacca
     1801 tgcgaggctt ggggcgcgat gaccgatatc aaccaccgca ttctgttgca gacccggccg
     1861 acgggaattc cagagccggg caatttcaca gccgatagcg tgccgattcg ggcgccggtt
     1921 acagg
//
LOCUS       DASWQN010000126         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_277870, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000126 DASWQN010000000
VERSION     DASWQN010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277870"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VGC82_02095"
     CDS             complement(<1..871)
                     /locus_tag="VGC82_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422096.1"
                     /translation="MDAEPNTIPDMNDPDLNRADLNGADPNDANLVLGFESIGDNCEL
                     GLLQRRAGVEPLGLLRFAGAPLRNLVHALNARFAGLADPARIRIEAENGEYLVKLAAY
                     DFTHHADVKIGEMEPAAVLQQQCRVVRFLTAKLIDDLENPNKILVFRQNESLAAGDLV
                     DLRIALAAYGPNILLWVQEACAGHPPGSVDVADERMMVGYVRRLARRHDVPDLDVASW
                     LQVLRQARAIAAKAVDRRLVRPPPNAAGRTVLTFGREGNAGPCLGAGWSDPEPGYTWA
                     VGERSTVTVANPGD"
     gene            complement(1206..2627)
                     /gene="nuoN"
                     /locus_tag="VGC82_02100"
     CDS             complement(1206..2627)
                     /gene="nuoN"
                     /locus_tag="VGC82_02100"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY0422097.1"
                     /translation="MNWTLALPEIVLACVGLATLVFGVLRKQDSTLLCTMFTIGGYLV
                     AGLLVLTRTNGFGFHGQFVADPFSAFNQILIISGAALSSIMALDWNRSQGIARFEFPV
                     LMLFSTVGMLIMAGASNLMTLYLGLELQSLALYVLAAFARDDLRSSEAGLKYFVLSGL
                     ASGLLLYGISLVYGFSGTMDLESLHGLLVQPSSATPGLVVGIVFVLVGLAFKVSAVPF
                     HMWTPDVYEGAPTPVTVFFATAPKVAAMAVLLRVMGTSFGSLVGAWQSLVIIVSIASM
                     LLGALAAIGQSNIKRLMAYSSIGHMGYALIGLAVGNAEGVRGTLVYMVIYVFMTAGTF
                     GCILAMRRKGRAVEQISDLSGLASNDPALALALAVFMFSLAGIPLMSGFFAKLYIFLA
                     AVQGGLWTLAVVGVLTSVVGAFYYIRIIKVMYFDAPAEHFDARPASLSFVVAATGLFT
                     GFFFLFPAPVVAAAQAAASVLFR"
     gene            complement(2624..>3679)
                     /locus_tag="VGC82_02105"
     CDS             complement(2624..>3679)
                     /locus_tag="VGC82_02105"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY0422098.1"
                     /translation="IGVWGGPRRVYAAVKFFLFTLTGSVLMLLALVAMWLQAGTTDIA
                     TLMHTPFPPGMQNWLFVALFASFAVKVPMWPVHTWLPDAHVEAPTAGSVILAGVLLKM
                     GAYGFLRFSVPMLPQAAASFAPFMFTLSVVAVIYTSLVALAQEDMKKLIAYSSIAHMG
                     VVTIGIFTFNVQGISGALFQMLSHGVVSAALFLVVGVVYDRIHSRDIARYGGLADRMP
                     GYALVFMLFMMASVGLPGTAGFVGEFLVIVGSLQVNFWLSLLGGMGMILGAAYMLYLY
                     RRVIFGRITRDDLRNILDLSPREWAVFAPLIVLTLWMGIYPSSFTSYFDATVGAMVQQ
                     HTAALAATTKLAGIVHQ"
BASE COUNT          821 a         1203 c         1098 g          557 t
ORIGIN      
        1 catcgccggg gttcgcgaca gtaacggtgc tacgttcgcc gacggcccat gtgtaaccgg
       61 gctccgggtc agaccagccg gcacccaggc agggtccggc attcccttcc cggccgaacg
      121 tcagcaccgt ccggccggcg gcgttcggcg gtggacgaac cagccgccgg tccacggcct
      181 tcgcggcgat cgcgcgcgct tggcggagca cctgcaacca tgacgccaca tccaggtcgg
      241 gcacgtcgtg ccgccgggcc agccggcgga catagccgac catcatccgc tcatccgcca
      301 catcgactga tcccggcgga tggccggcac aggcttcctg aacccagagc aggatgttcg
      361 gaccataggc agccagtgcg atccgcagat cgaccagatc gccggccgca agcgattcat
      421 tctggcgaaa aaccaggatc ttgttcggat tttcgaggtc gtcgatcaac ttggcggtca
      481 gaaaccggac cacgcggcat tgctgttgga gcacggccgc cggctccatt tcgccgattt
      541 tgacgtcggc gtggtgggtg aagtcgtagg cggccagctt gaccaggtac tcgccgttct
      601 ccgcctcgat acggattcgg gcggggtccg cgagaccggc aaaacgcgca ttcagggcgt
      661 gcaccaggtt gcgcagcggc gccccggcaa agcgcagcaa tcccagcggt tcaacgccag
      721 cccggcgttg cagcagcccc agctcacaat tgtcgccgat gctttcgaac ccgagcacca
      781 ggttcgcgtc gttcgggtcc gccccgttca ggtccgccct gttcaggtcc gggtcgttca
      841 tgtccggaat ggtattcggt tcagcgtcca ttccgcgtat cgcatctcat gcagaccatg
      901 cgaacgatgc tatagggata tggtggcgcg cttcaagaaa aactcgtttc tggagcattt
      961 tcagcgtgac cgtacaggct gaaaatgctc tatctctttg tttgatcgca tgatttacac
     1021 gctttgaacg ttccgctcag cgtgatcatg ctctaaccag gtcggttgca ttcggaccag
     1081 caaagcgact ggcaggtttc cggtcatgcc ggtggtggtc cggcgaaagc gagactcaag
     1141 caggcaaatt tgccaaacca cgcttcatcc gcggctgcag tcttgcgccg gtgtgactca
     1201 agacgctacc ggaacagcac gctcgctgcc gcctgcgccg cggcgaccac cggggcggga
     1261 aacaggaaga aaaatccagt gaacaaccca gtcgccgcca ccacgaagga taacgaggct
     1321 ggccgtgcat caaaatgttc cgccggcgcg tcgaaataca tgactttgat aattcggatg
     1381 taataaaatg ccccgaccac gctggtcagc acgcccacca ccgccagcgt ccacaatccg
     1441 ccctgcactg ctgccaggaa gatatacagc ttggcgaaga acccggacat cagcggaatg
     1501 ccggccagac tgaacatgaa caccgccagg gccagcgcca aagccggatc gttcgacgcc
     1561 aatcccgaca gatcggaaat ctgctccacc gcccgtccct tgcgccgcat tgccaggatg
     1621 cagccgaacg tgcccgcggt catgaacacg tagatgacca tatagaccag cgtaccgcgc
     1681 acgccctccg cgttgccgac ggccagacca atcagcgcgt agcccatatg gccgatcgag
     1741 gagtaggcca tcagccgctt gatgttgctc tggccgatcg ccgccaacgc gcccagcagc
     1801 atcgaggcga tcgaaacgat aatcaccaga gactgccacg cgccgaccag cgagccgaac
     1861 gaggtgccca tcacccgcag cagcaccgcc atcgccgcga ccttcggggc ggtcgcgaag
     1921 aataccgtca ccggggtcgg cgcgccctca tacacatcgg gcgtccacat gtggaacggc
     1981 accgcggaga ctttgaaggc caatccgacc agcacgaaca cgatccccac caccaagccg
     2041 ggcgtcgcac tggaaggctg caccagcagc ccgtgcagcg attccagatc catggtgccg
     2101 gagaacccgt acaccagaga aatgccataa agcagcaggc cggacgccaa gccgctcagg
     2161 acaaaatact tcaggccggc ctcagacgat cgcaggtcgt cgcgggcgaa tgcagccaac
     2221 acgtaaagtg ccagcgattg cagttccaga cccaggtaca gcgtcatcag gttcgacgcc
     2281 cccgccatga tcaacatgcc gaccgtggaa aacagcatca gcaccgggaa ctcgaaccgg
     2341 gcgatgccct gggagcggtt ccagtccaat gccatgatgg aggacagcgc cgcgccggag
     2401 atgatcagaa tctggttgaa cgcgctgaac ggatcggcca cgaactggcc atggaagccg
     2461 aaaccgttgg tgcgcgtcag caccagcagg ccggcgacca gatagccgcc gatggtgaac
     2521 atcgtgcaca gcagcgtgct gtcctgtttg cgcaggacgc cgaagaccag cgtcgccagg
     2581 cccacgcagg cgaggacgat ctcgggcagg gccaaggtcc agttcattgg tgcacgatcc
     2641 cggcaagctt ggtggttgca gccaaggcgg ccgtgtgctg ctgcaccatc gccccaacgg
     2701 tcgcgtcgaa atagctggtg aagctggacg ggtagatgcc catccacagc gtcagcacga
     2761 tcagcggtgc gaacaccgcc cattcgcgcg ggctgaggtc caggatgttg cgaagatcgt
     2821 ccctggtgat acggccaaaa atcacccggc gatacaggta cagcatgtag gccgcgccca
     2881 ggatcatgcc catgccgcca agcagggaca gccagaaatt cacctgcaac gagccgacga
     2941 tgaccaggaa ctcaccgacg aagccggcag tacccggcag gccgaccgag gccatcatga
     3001 acagcatgaa aaccaacgcg taaccgggca tccggtccgc caggccgccg tagcgggcaa
     3061 tgtctcgcga gtggatgcgg tcataaacga cgccgacgac gaggaacaag gccgcggaaa
     3121 cgacaccgtg cgacaacatc tggaacagcg cgccggagat accctgcacg ttgaaggtaa
     3181 agataccgat cgtcacgacc cccatatggg cgatcgagga ataggcgatc agtttcttca
     3241 tgtcctcctg tgccagcgcg acgagcgacg tgtagatcac cgccacgacc gacaacgtga
     3301 acatgaacgg cgcaaaggac gccgccgcct gcggcagcat aggcaccgag aagcgcagga
     3361 acccgtaggc gcccatcttc agcaacacgc ctgccaggat gactgagccg gcggtcggcg
     3421 cctccacatg cgcatccggc agccaggtgt ggaccggcca catcggcacc ttgaccgcga
     3481 aggaggcaaa cagcgcaacg aacagccagt tttgcatgcc gggcggaaac ggggtgtgca
     3541 tcagcgtggc gatgtcggtg gtgccggcct gcagccacat cgcgaccagc gccagcagca
     3601 tcagcaccga accagtcagc gtgaagagga agaacttaac cgccgcgtaa acccgtctcg
     3661 gccctcccca gacgccgat
//
LOCUS       DASWQN010000127         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1993129, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000127 DASWQN010000000
VERSION     DASWQN010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993129"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VGC82_02110"
     CDS             complement(<1..373)
                     /locus_tag="VGC82_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0422099.1"
                     /translation="MSRNGLPALGFHAAFIAFMLAPLFAVCLVAFTPENYLSVPYDAF
                     STRWFRTLLHRPDFLRAAWLSVRLAAAASTLAIVVSVPASLVSARYRFAGQNALGALF
                     MSPLTIPHVVLGIALLRYFIQI"
     gene            complement(370..1224)
                     /locus_tag="VGC82_02115"
     CDS             complement(370..1224)
                     /locus_tag="VGC82_02115"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0422100.1"
                     /translation="MMRLVRVWLPNAPALVLYTGFLVTPLIMTGLLSFQQNNQAAEVL
                     GPLTLANWREVFADEYYLGIFARTLRIACLVTGFAIGLGATEAYILSRMRAPWRSLFL
                     LIVLTPLLISVIARTLGWALLFGPNGPLGNLPVWLGLSRQPLQFLFSETGIEVALIHV
                     LTPFMVLSVWASLQQLDPQVENAATSLGASRLAAFRRVILPQILPGVLSGAIIVFTFS
                     SSAFATPAIIGGRRLKVVSVLVYDEFVHQMNWPLGAALSMALLLSLGLATAAASRLVE
                     RRATRGGE"
     gene            complement(1221..>1610)
                     /locus_tag="VGC82_02120"
     CDS             complement(1221..>1610)
                     /locus_tag="VGC82_02120"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY0422101.1"
                     /translation="FVAQFLGRTNLLPGRVRSESGRDHLQVGEACWALPYSHDSGSVL
                     VTVRPERVVFTSFDGNGLLGTVRARIFHGHEWLHRVETCGGIVTVVTPNGSLPMPAEG
                     ETVRLYWDAGDITLTQDAGIIAREPRE"
BASE COUNT          377 a          485 c          504 g          244 t
ORIGIN      
        1 caatctggat gaaataccgt agcaaggcga ttcccaggac cacatgagga attgtcagcg
       61 gcgacataaa caaggcgccc aaggcgtttt ggcccgcgaa gcgatagcgt gcggagacca
      121 gggaagctgg taccgaaacg acaatggcca gagtggatgc ggcggcggcg agccgaacgc
      181 tgagccacgc cgcacgcagg aaatcaggcc ggtgcagcag cgtacggaac cagcgtgtgc
      241 tgaaagcatc gtacggcacc gacaggtagt tctccggcgt gaacgcaacg aggcatactg
      301 cgaaaagcgg cgccagcatg aaagcgatga aagccgcatg aaaacccagc gccggcaggc
      361 cattgcggct cattcgccgc cccgggtcgc tcggcgttcg accaagcgac tggcagcggc
      421 ggtcgccagt ccgagtgaca gcagcagcgc catcgacagg gcggcgccga gcggccagtt
      481 catttggtgg acgaattcat cgtacaccag cacggacacg accttaagcc gccggccgcc
      541 gatgatcgcc ggagtcgcga aggcgctgga agaaaaagta aacacgatga ttgctccgga
      601 cagaacgccc ggcagtatct gcggcaggat cacgcggcgg aacgccgcca gccgggatgc
      661 gccaagggaa gtcgcggcgt tctcaacctg tggatcaagc tgctgtagcg aggcccacac
      721 tgacagtacc atgaatggcg tcagtacatg aataagcgcg acctcgatcc cggtttcact
      781 gaacaggaac tgcagcggct gccgcgataa accaagccac accggaagat tcccgagcgg
      841 tccgttcggg ccaaaaagca atgcccagcc caaggtgcga gcaatgaccg agatcagcaa
      901 gggagtcagc acgatcaata gaaacagcga gcgccatggc gctcgcatgc ggctcaggat
      961 gtaggcctcg gttgctccca agccgatcgc aaaacccgtg accaggcatg cgatcctcaa
     1021 ggtgcgtgcg aagatgccga ggtagtattc atcggcaaag acctcgcgcc agttcgcgag
     1081 cgtcaacggg cccaaaacct ccgcggcctg gttattttgc tggaaggaga gcagcccggt
     1141 catgatcaaa ggcgtgacca ggaaacccgt ataaagcaca agtgccggtg cattgggtag
     1201 ccagacccgg accaggcgca tcattcccgg ggttctcgtg ctatgatgcc tgcatcctgc
     1261 gtcagtgtaa tgtccccggc gtcccagtac aggcggacag tttcgccctc ggccggcatt
     1321 ggcaggcttc cgttcggcgt gacgacggtt actatgccgc cacacgtctc tacccggtgc
     1381 agccattcat ggccatgaaa gatgcgggcg cgcacggttc caagcagtcc gttcccgtcg
     1441 aagctggtaa atacgacccg ttccgggcgg accgtgacca agaccgagcc gctatcgtgg
     1501 gagtagggca gggcccagca ggcttcccca acctgcagat ggtctcgtcc gctctcgctg
     1561 cgaacacgcc ctggcagcag gttggttcgg ccaagaaact gggcgacgaa
//
LOCUS       DASWQN010000128         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1465506, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000128 DASWQN010000000
VERSION     DASWQN010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465506"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VGC82_02125"
     CDS             <1..374
                     /locus_tag="VGC82_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422102.1"
                     /translation="WMPINYLLIESLYRFGSFYGSDTRFECPTGSGQYRTLPEIAEEL
                     ARRLTRLFLRGPDGRRPVLGFNDQLQMDPAFRDHVPFHEYFHGDTGAGLGAGHQTGWT
                     GLIALLLFPDLQARIRPPPYE"
     gene            complement(544..747)
                     /locus_tag="VGC82_02130"
                     /pseudo
     CDS             complement(544..747)
                     /locus_tag="VGC82_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612221.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(824..1438)
                     /locus_tag="VGC82_02135"
     CDS             complement(824..1438)
                     /locus_tag="VGC82_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HEY0422103.1"
                     /translation="MSLPLLPGFLERHPLIRPEWHFENRRVDLVTEGYDAVIGAGFDL
                     SPGLIAHTLAPAHVIAVASPAYLEGRLLPDDPTGLRAFQGIVMRSLRTGRLQQWTMRH
                     AGGAEKPGPLTASIVVNDPDAMQEAALLGLGVTMLAVPDVLPALETGRLLRLVPGWYA
                     DAGAISLYYNSRTLLPAKTRAFVDWVTEAFKDRRLAERFAGSVV"
BASE COUNT          331 a          585 c          625 g          318 t
ORIGIN      
        1 tctggatgcc gatcaactac ctgctgatcg agtccctcta tcgatttggc agcttctacg
       61 gcagcgatac gcggttcgaa tgcccgacag gatccgggca ataccgcacc ctaccggaga
      121 tcgctgagga actcgcacgc cgcctgacgc gcctgttcct gcgcggtccg gatgggcgcc
      181 gtccggtgct tggattcaac gaccagctcc aaatggaccc ggccttccgc gaccacgtgc
      241 cattccacga gtacttccac ggcgacaccg gggcagggct tggggcaggg catcaaaccg
      301 gatggaccgg gctcatcgcg ttactgctgt tccccgatct acaggctcgg atacgccctc
      361 caccctacga atagggccag agttccgctt ctgaccaatt ttgctgagaa cgcagggtac
      421 ggctggagcg gcccacgcga cggagttcga aggagaaggg ttccctccag ggtctggact
      481 cgatctgcac cagaaggcga tcacggcagt gcgtgcgatg gccggggcct cgttgcgcgc
      541 gagcttgtcg tagcgggtgg cggtgcgcgg accgtattcc gccgggcagt cacgccagcg
      601 gcagcctgag gtcagcagat gcacgatgcc cgacaggatc atccggtcgt ccttgcgttc
      661 ggggccgggc tgattgacgg gcatgcgggc atgaatggcg cgatcaccgc ccactgcgca
      721 tccgagatgc cagaataggt ccgccatcac cgccgccatg ctgcaaacgg ccgccctgaa
      781 tcacagaacg aagcggtcgc aaaaccgtgg ttcggttcag attctagacc acgcttcctg
      841 cgaagcgttc tgcaagacgc cgatccttga aagcttcggt gacccagtcg acgaaagcgc
      901 gtgtcttggc aggcagcagc gttcggctgt tgtagtagag agagatcgct cccgcatctg
      961 cgtaccaacc cggcaccagc cgaagcagac ggccggtttc cagcgcaggc aggacgtcgg
     1021 ggaccgcgag catggtgacg cccagcccga gcagggcagc ttcctgcatc gcgtccgggt
     1081 cgttgacgac gatggatgcg gtgaggggcc ccggcttttc cgcgccccct gcatgacgca
     1141 tggtccattg ctggaggcgg ccggtccgga gcgagcgcat gacgatgcct tggaacgctc
     1201 ggagtccggt tggatcgtcg gggagcagcc ggccttccag gtaggcgggc gaggccacgg
     1261 caatgacgtg cgccggggcc agggtatggg cgatgaggcc gggggacagg tcgaaccctg
     1321 caccgatcac ggcgtcgtag ccctccgtga cgagatcgac ccgacggttc tcgaagtgcc
     1381 attccgggcg gatcagggga tggcgctcga gaaaaccggg cagcaacggc agggagatgc
     1441 atcgtgccga atgtcggggc catgctgacc ttgagggtgc cggccggctc gcctcctacg
     1501 gtggcgatgc cggcgatcgc cgcctgaatg ccctccagcc cgtcggcgat cgcgaggcgg
     1561 aaggtttcgc ccgcttccgt caggctcagc ttccgcgtcg agcggtggaa cagacgcacg
     1621 ccgaggttcc gctccagcat ggcgacgttc cggctgatgg cggccggcgt cagcgacaag
     1681 cgccgggcgg cttcggagaa gctgccgtgc tcggcgctgc ggatgaagga ttccaggttg
     1741 gccagggttt ccatttggcg ccactgacag cggtgttaca tcgcctcgcc atgccgacag
     1801 agtgcgcacc gacccgctaa gatatcctga atttctgcgc gactattccg ccataaggg
//
LOCUS       DASWQN010000129         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_14563, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000129 DASWQN010000000
VERSION     DASWQN010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14563"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..795)
                     /locus_tag="VGC82_02140"
     CDS             complement(82..795)
                     /locus_tag="VGC82_02140"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0422104.1"
                     /translation="MTDTIRVMTWNVHGTFNLNPNFDLEAVCSIIRKWSPDIVALQEV
                     DSRGRTDDVFGRLAATVGDYRVEARSIVTRDGDYGQVLLSRWPFASEPKVTDVSYQEK
                     EPRRAIAARVRAPLWEVTVVATHLGLSIYERYAQAQALAALVDAPRTIIIGDFNDWFW
                     VKSVRRVLATRCPIRTRLRTFPSRFPMLRLDRIYATPSGTIMKAWTDKEAARCSDHLP
                     VFADIAFEARGEKGETRKV"
     gene            complement(792..>1560)
                     /locus_tag="VGC82_02145"
     CDS             complement(792..>1560)
                     /locus_tag="VGC82_02145"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY0422105.1"
                     /translation="FAHTPALTDVVQPIADPRHPLNLHRAARQMWNRRTVIAAAGTAV
                     ALAGLALAWRTTSLSHYTDPGYVSSLIAQYAQSAFGPLYAVALFVLGGLLVFPVIILI
                     AATAAALGPWIGALSATVGVLLSSLLLFMIGRLLGHKRLQGVLGTRALRIQNRIVGRG
                     VVAVAMIRMVPIAPFTLVNVLAGSSQLRLSDFLIGTLLGMAPGIITMALLGAQIADFS
                     RNASWSNALPLALTIVLWITVCLAVQFLVTWITGRRR"
BASE COUNT          349 a          465 c          456 g          290 t
ORIGIN      
        1 gtggatggcg gcaccaatac ccccgtgccg gcgtctcttt gacgcctgct gactatccag
       61 agaaaatcaa acccttgttc actacacttt tcgtgtttcg cccttctcgc cccgtgcctc
      121 aaacgcaatg tcggcgaaca ccggcaaatg atccgaacag cgcgccgctt ccttatcagt
      181 ccaagctttc ataattgttc cactcggcgt ggcgtagatc cgatctagac gcagcattgg
      241 aaatcgcgat ggaaaggttc gcagtcgcgt tcgtatggga cagcgtgttg ccagcacccg
      301 ccgcacagat ttcacccaga accagtcgtt gaagtcgccg atgatgattg ttcggggcgc
      361 gtccaccagt gctgcaagcg cctgcgcctg tgcatagcgc tcgtatatgc tcaagccgag
      421 atgggtcgct acgacggtta cctcccatag cggtgcacgg acgcgcgcgg cgattgcccg
      481 gcgcggctcc ttttcctgat atgacacgtc ggtcaccttg ggttcgctgg cgaacggcca
      541 ccggctgagc aggacctgac cataatcgcc gtctcgcgtg acgatagaac gggcttcaac
      601 gcgatagtcg cccacggtgg ctgccagtcg tccaaacaca tcgtcggtgc ggccgcgcga
      661 gtcgacttcc tgaagggcga cgatgtcagg cgaccatttg cgaatgatcg aacagacggc
      721 ttcaagatca aagttgggat tgagattgaa cgtgccgtgc acattccatg tcatgacgcg
      781 gatcgtatcg gtcatctgcg tcggccggtt atccaagtca ccaggaattg taccgcaaga
      841 cacaccgtga tccacaatac gatcgtcagg gcgagcggca gcgcattcga ccaggaggca
      901 tttctggaga agtccgcgat ctgcgcgccc agcaaggcca tcgtgatgat gcccggcgcc
      961 attccaagca gggttccgat caaaaagtcg cttagccgaa gttggcttga gccggccaga
     1021 acattgacca gcgtaaaagg agcgatcgga accatccgga tcatcgccac cgcgacaacg
     1081 ccacggccga cgatgcgatt ttggatacgc aaggcgcggg tgccgagcac accctggagg
     1141 cgtttgtgac caagcagacg accaatcata aacagcaaga gtgagctcaa gagaaccccg
     1201 accgtagcac tcaaggcgcc gatccatggc cccagcgccg ccgccgttgc ggcaataaga
     1261 atgattacgg gaaatacgag cagcccgccc agcacaaaaa gggctaccgc atagagtggc
     1321 ccaaatgccg attgtgcgta ttgcgcaatg agggaggata cgtatccggg atcagtatag
     1381 tggctcaacg acgtggtgcg ccaagcgagg gccagccccg cgagcgcgac cgccgtaccc
     1441 gccgccgcta tcacggtacg cctattccac atttgacgcg cggcgcgatg aaggttgagg
     1501 ggatgcctcg gatctgcgat gggctggacg acgtcagtca gtgccggtgt atgcgcgaaa
//
LOCUS       DASWQN010000130         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2125873, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000130 DASWQN010000000
VERSION     DASWQN010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125873"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VGC82_02150"
     CDS             complement(<1..425)
                     /locus_tag="VGC82_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0422106.1"
                     /translation="MALSHQPSAVRNRLLAALPPDVLAQFLPKLHPISLPLRKVLNSP
                     GEPIEAVYFVESGWVSMVTHLDEGAQAEVGLVGREGLVGASIATGIDTAFAEAYVQAP
                     GEALQMETKAFQRELDTHPALFRRLLRLYRGDARSGHAD"
     gene            882..1265
                     /locus_tag="VGC82_02155"
     CDS             882..1265
                     /locus_tag="VGC82_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0422107.1"
                     /translation="MASSVLVFVVEDQEIIRHVLEEALTDGGFAVAMATDGEQAMAML
                     DAKEADYSALVTDVNLPGKFSGWDVAKHAREINDKIPVIYITGASAHDWASRGVPNSQ
                     LLPKPFAVVQVVTAVSQLINTANSG"
     gene            complement(1473..2654)
                     /locus_tag="VGC82_02160"
     CDS             complement(1473..2654)
                     /locus_tag="VGC82_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422108.1"
                     /translation="MLAGTSAVKAQPAPVATESVKLGMILDMGSLYADVTGPGSELAA
                     RMAVADFGGKVLGRPIEVLVADHQTKADIAASIATKWFDTENVVALLDVAASSPALAV
                     MNVANIRGKIVLLNGPGASSITNESCIATAVHYAYNTYATAHAVTKAIIDQGGKNWYY
                     LTADYTFGHQLESDSATVVKANGGEVLGDSKAPINTSDFSSFLLEAQQSKAQVIGLAN
                     AGSDLINSVKQAAEFGITRHGSKLASLAANINDIYGIGLDATQGMILSDAFYWDTDDQ
                     TRAFSKRFFDKIKKMPNMLQAAVYSSTMHYLQAVQASGTTDSGPVMQKMRDTPIHDFF
                     AHDGHIRVDGLMVHDMYLFQVKTPTEAKGPWDLYKRLATIPGDQAFQPLSLSRCPLVK
                     K"
BASE COUNT          622 a          878 c          831 g          654 t
ORIGIN      
        1 ctgtctgcat ggcctgagcg tgcatcgcct cggtatagcc gaagcagtcg ccggaacaag
       61 gcgggatgcg tgtccaattc gcgctgaaat gccttcgtct ccatctgcaa agcctcaccg
      121 ggggcttgca cataggcttc agcgaaagct gtatcgattc cggtggcgat agaggcacca
      181 accaagcctt cccgaccgac caatccaacc tcagcctgag cgccttcgtc taaatgcgtc
      241 accatcgaga cccagccact ttcgacaaag taaacagcct cgatcggctc tccagggcta
      301 ttaagaactt tgcgcagagg cagggaaatc gggtgcagct taggcaggaa ttgagccaga
      361 acatcaggcg gtaaagccgc gagaagacgg ttgcgaacgg cggatggctg atgggacagg
      421 gccatattgg ctcctcgcac ttgtgtggcg ggagcactgg cctctctcaa gcacccgttg
      481 ccgaaggccg cggcgggcga taactcgata gtggcgattg cctggagggc atgcaacccg
      541 ttggttaatc gcaaaattga actgcggttg cgcggccttg gtacgaagat ccgcaagatc
      601 ataatggtgg cggacaaatt ctgtatgttg tgctcgcacg ctcccgctca tgcgacacgc
      661 ttatgcgaaa tcaccggcgc atattgcctg ttctgttaaa ttctttcgtg tatcgaaatt
      721 ttcaagattt gcacgaaggt ggataagggg ccacgtcctc ggggcctcgt cataccgtcc
      781 cgctgtgtta gggttataaa gatcataggt catccggtcg tgtctgggag ttccggttcc
      841 tcgagccaca tgctccccat cgtccggcaa ggagtaacgc tatggcctca tccgttctcg
      901 ttttcgttgt cgaagatcag gagattattc ggcatgtgct ggaagaggcg ctaacagatg
      961 gtggttttgc cgttgctatg gcgactgatg gcgaacaagc aatggccatg ctggacgcaa
     1021 aggaagcaga ctatagcgcc ttggttacgg atgtgaacct tcccggaaaa ttctcgggct
     1081 gggacgtggc taaacatgcc cgcgaaatca atgataagat acctgtcatt tacataaccg
     1141 gggcgagcgc acacgactgg gcctcaagag gagtccccaa tagccagctg ttgccaaaac
     1201 ctttcgccgt cgtacaagtc gtaacggctg tttcacagct catcaacacg gcaaactctg
     1261 gctaaaggac agctcactgg tcaaattctt gatatttgcg tgcgcccatc tctgcggcgc
     1321 caccgccctg ggctctgatt ctcaggcgat tcccgaaact cgagaagtac gattctctcg
     1381 ccgttagact atatgcctgg gggtcggcgc acgcgcggta agctgcgcga cgctgacccc
     1441 gagttcttca accaaccgga gttttccgcc agttattttt tcaccagtgg gcagcgggat
     1501 aacgagagcg gctggaacgc ctgatcgccc ggaatggtag ccaggcgctt atacaggtcc
     1561 catggtccct tagcttccgt cggcgtcttc acctggaaca aatacatgtc gtgcaccatc
     1621 agcccgtcca cgcgaatatg gccatcgtgg gcaaagaaat cgtggatcgg cgtgtctcgc
     1681 atcttctgca tcacagggcc actgtccgtg gtgccggacg cctgtactgc ctgcaggtag
     1741 tgcatggtcg acgaatagac cgccgcctgc agcatgttcg gcatcttctt tatcttgtcg
     1801 aaaaaccgct tcgagaacgc gcgggtttgg tcgtccgtgt cccaatagaa cgcgtcgctc
     1861 aggatcatgc cttgggtcgc gtccagccca atgccgtaga tgtcgttgat gttggcggcc
     1921 agactggcaa gcttgctgcc gtggcgggta ataccgaact ctgcggcctg cttgaccgag
     1981 ttgatcaagt cgctgccggc attggccaag ccgatgacct gcgccttgga ttgctgggcc
     2041 tccagcagga acgacgaaaa gtccgatgtg ttgatcggcg ccttcgaatc gccgagcact
     2101 tcgccgccgt tggccttgac gaccgtggcg ctgtcgctct ccaactgatg cccgaaggta
     2161 taatcagcgg tcaggtagta ccagttcttg ccaccctggt caatgatggc cttggtcaca
     2221 gcgtgcgccg tcgcgtaggt gttgtaggca taatggacgg cggtcgcgat gcacgattca
     2281 ttggtgatcg acgacgcgcc tggtccgttc agcaacacga tcttgccgcg gatattagcg
     2341 acgttcatga ccgccagcgc gggcgacgaa gccgccacat cgagcagcgc aaccacattt
     2401 tcagtgtcga accatttcgt cgcaatcgac gcagcaatgt cggccttggt ctggtgatcg
     2461 gctaccaaca cctcgatcgg ccgacccagc accttgccgc caaaatccgc gaccgccatc
     2521 cgcgccgcca attcactgcc cgggccggtc acatcggcat acaggctacc catgtcgagg
     2581 atcataccga gctttaccga ttcggttgct actggcgccg gttgcgcctt aacggcgctg
     2641 gtgccggcca acacaaagca ccccaaaaga gcaagatata aatgtctcat aggcatttct
     2701 ttcccaccgt cccccaacta tcggctggct tcacggctct gcaaagcccg tgtctcaagg
     2761 gacggccaag agcttcacac cgcatcgctg ccgttgttgg caaaagtctt gagttttttc
     2821 aaaggccgcc ttagggtttt tgtcatatga cgaaattctt ccatcggcgg taatcgccca
     2881 gttgcccccg gccgtgtttc tccgccaacc ctaacggctg cccgcatccg tgcgcgacat
     2941 gcgcacccag ccgcgcaacg tctccgccgt gcagccgatc ttggc
//
LOCUS       DASWQN010000131         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2389651, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000131 DASWQN010000000
VERSION     DASWQN010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2389651"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="VGC82_02165"
     CDS             <1..1199
                     /locus_tag="VGC82_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0422109.1"
                     /translation="MLRSNVSRATSPFWNTDWRNSSVAAILESIQRAAQGQRAAQGQN
                     AAQGGKSPSDFPSAVVAETMRQDAALIEGIAAYRRHPAYRTLEDPPCIWSEGSTRLLD
                     YGPPAGRPVVFVPSLVNRAYVLDLAEGNSMLRWLALRGIRPLLLDWGWPEETERRFNL
                     TDYVAGRMERALTAAVRAVGVPPVLAGYCMGGTLAVAAAQRRPDLIGGLALLAAPWDF
                     HAPEADRATQLAKTLPLLEPALAFSRALPVDLLQSLFALLDPWSVADKYRAFARLPPE
                     SERARLFVALEDWLNDGVPLAADVARVCLREWYGENAPARGTWRIAGLPVDPASIATP
                     TFVAVPDRDRIVPPESARPLSRLIPGAVLHQPAAGHIGMAAGARAEAVLWRPLLDWLE
                     TLGRSI"
     gene            1266..>1869
                     /locus_tag="VGC82_02170"
     CDS             1266..>1869
                     /locus_tag="VGC82_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEY0422110.1"
                     /translation="MAGKQPVAIVFDTFGTVVDWRSSLIADLSAYGSSRGVTADWPAL
                     IDAWRASYHPSMDRVRKGQLPWTTLDNLHRASLDHLVAEFGIKGLSEDDLVHINLGWH
                     RLHAWPDAVPGLTRLKSRFIIGPLSNGNVALLLNMAKFAGIPWDMIFGSDLFGHFKPD
                     PETYLGVARMLNLRPDQVMMGAAHNGDLGAARSCGLMTAFF"
BASE COUNT          286 a          590 c          643 g          350 t
ORIGIN      
        1 cgatgctgag atcgaacgtc tcgcgcgcca cgtcgccctt ctggaacaca gactggcgga
       61 actcgagcgt ggccgcgatc ctggagtcga ttcaacgcgc agcccagggt caacgagcag
      121 cccagggtca aaacgcagcc cagggcggaa aaagccccag tgacttccct tccgccgtgg
      181 tggccgagac catgcgccag gatgcggcac tgatcgaagg catagcggcg tatcgccggc
      241 atcccgcgta ccggacgctg gaggatccgc cttgcatctg gtcggagggc agcacccggc
      301 tactggatta cggccctccg gccgggcggc cggtcgtgtt cgtaccaagc ctggtcaatc
      361 gagcctatgt gttggatttg gctgaaggga actcgatgct gcgctggctc gcgttgcgcg
      421 gcatccgtcc gctgctgctg gattggggct ggccggagga aaccgaacgg cggttcaacc
      481 taaccgacta tgttgcgggc cggatggagc gggcgctgac tgccgccgtt cgtgccgtcg
      541 gtgtaccgcc ggtgctggca ggctactgca tgggtggcac tttggcggtg gcggcggcgc
      601 aacgccggcc cgacctgatc ggtggcctcg cgctgctggc ggcgccgtgg gatttccacg
      661 cgcctgaagc ggatcgggcg acccagttgg caaagacctt gccgctgctg gagccggcgc
      721 tggccttcag ccgtgcgctt cctgtcgatc tgctgcaatc gctgttcgcg ctgctcgatc
      781 cctggagcgt tgccgacaaa taccgcgcct tcgctcggtt gccgcctgaa agcgagcggg
      841 cccggttgtt tgtggcgctg gaagattggc tgaacgacgg cgttcccctc gcggccgatg
      901 ttgcacgcgt gtgtctgcgc gaatggtatg gcgagaatgc accggcgcgg ggcacctggc
      961 ggatcgccgg cctgcctgtc gatcccgcat caatcgcaac gccgactttc gtcgcggtgc
     1021 cagaccgcga caggatcgtg ccaccggaat cggcccgtcc gctctcccgg ttgattccgg
     1081 gcgctgtgct gcatcagccg gctgcgggtc acatcggcat ggccgccggt gcgcgggccg
     1141 aggcagtgct gtggcggccg ctgctcgact ggcttgaaac cctggggcga tcaatctgac
     1201 tggcgggcct gcttgaccgc gaaccgccgt ggggttctcc tgcggcgaca agtggggagg
     1261 ttctgatggc cggcaaacaa cccgtggcga tcgtcttcga tacgttcggc acagtggtcg
     1321 attggcgatc cagtttgatc gccgacctca gtgcctatgg gtccagccgt ggtgtgaccg
     1381 ccgactggcc ggcgctgatc gatgcttggc gggcctccta ccatccgtcg atggaccgcg
     1441 tccgcaaagg gcaactgccc tggacgacgc tggataacct gcaccgcgcc tcgctggacc
     1501 acctggtggc cgaattcggc atcaagggct tgtcggagga cgatcttgtc cacatcaacc
     1561 tcggctggca caggttgcac gcatggccgg acgccgtgcc cgggctgacg cggttgaaat
     1621 cccggttcat catcgggccg ctttcgaacg gcaacgtcgc cctgctgctg aacatggcga
     1681 aattcgcggg catcccgtgg gacatgatct tcgggtcgga cctgttcggg catttcaagc
     1741 cggacccgga aacctatctg ggcgtcgcga ggatgctgaa cctgcggccg gaccaggtga
     1801 tgatgggggc ggcacataat ggggacctgg gcgccgcccg atcctgcggg ttgatgactg
     1861 cgttcttcc
//
LOCUS       DASWQN010000132         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_674207, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000132 DASWQN010000000
VERSION     DASWQN010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674207"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VGC82_02175"
     CDS             complement(<1..300)
                     /locus_tag="VGC82_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0422111.1"
                     /translation="MPKLIPVPDALSKPFWDAVNQRRLVLQHCAACDRLQYPPQQACQ
                     VCDAAGGLTWQEVEGRGHIAAYIVIEDGRLNRRMPDQPYNLAMVTLDADPRVIFYS"
     gene            complement(305..2278)
                     /locus_tag="VGC82_02180"
     CDS             complement(305..2278)
                     /locus_tag="VGC82_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0422112.1"
                     /translation="MSDTHARPALARVAGAGHFPNCPQLRGRSHRRGSWETARGRSFG
                     APIRRPTARLRAEETPSMTIDVATGISRILKQEGVGWVSTFPVCRVNNAFGREGMPML
                     MMRDDRYAVAVADAFSRIMGGSEGGSPGENSRIGVCTFQGGVNAAGMQYAYAGLAQAY
                     EDGSPVLCITDGVPAGSSGNSQYDVASAMKTVSKWFGYLDKPERTPELMRRAFTMLRS
                     GSPGPVILAIPNATATYDETADPYRPVKGWKSAPDPADVRAAVDQLLAARNPLIYAGE
                     GVIYAGASEDLRALAELVNVPVITTLKAKGAFPENHKLFVGVRGDHVAHYLNKSDLIL
                     AIGSSLSPGRFSHAIPDAAKKTIIHCNIDELHINKMYSTAYAVIGDARLTLQALRQEV
                     SDRTEPSKAAVIPLDAAIQANLRRATTSALASSGAGQTMGDMAARNVAAEVKAARDEA
                     LGRYRQVMVSDEKPINPYRVYGDLMQVLDLRNSFVTHESGNTRDQLSTVFDTLIPRGF
                     LGWGNVSTLGFSLAAAIAAKKAFPDRASVAVTGEAGLGYMLGNLEVLLREKLGITVVH
                     ISNGGFAGYGPGFWGPGHDPFTHQVMGYDEVDMSKVIGALGFHSERVSEPAEVIPALR
                     RALDVNTSGRPAYIEFICSQYPVYGQWVGRSAE"
     gene            2225..2377
                     /locus_tag="VGC82_02185"
     CDS             2225..2377
                     /locus_tag="VGC82_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422113.1"
                     /translation="MTGTGHPGKSRSGMCVGHEIIPDKNGCVNEATAGLTLGLFVMPF
                     QDVAFG"
BASE COUNT          515 a          885 c          849 g          493 t
ORIGIN      
        1 ggaatagaaa atgacccgcg gatccgcgtc gagcgtcacc attgcaaggt tatagggctg
       61 atccggcatg cgccggttga ggcggccatc ctcgatcacg atgtaggccg cgatatggcc
      121 gcgtccctcc acctcctgcc acgtcagacc gccggccgcg tcgcagacct ggcaggcctg
      181 ctgcggcgga tactgcagcc tgtcgcaggc agcgcagtgc tgcaaaacaa gccgccgttg
      241 attgacggcg tcccagaacg gcttgctcaa agcatcgggg acggggatca gcttcggcat
      301 cgtttcactc cgcgctgcgt ccaacccact ggccgtaaac cgggtactgg gagcatatga
      361 actcgatata ggccgggcga cccgacgtat tgacgtcaag cgcccgccgc agcgccggga
      421 tgacctcggc cggctcgctc acccgttcgg agtgaaagcc cagggcgcct atcaccttcg
      481 acatatccac ctcgtcatac cccatcacct ggtgtgtgaa gggatcgtgt cccggtcccc
      541 agaagccggg tccatagccc gcaaagccgc cattgctgat gtgcacgacc gtaatcccga
      601 gtttctcgcg aagtagaacc tcaaggttgc cgagcatata gccaaggccc gcctcgccgg
      661 ttaccgccac ggatgccctg tccggaaaag ctttcttggc ggcaatggcg gcggccaggc
      721 tgaagccaag cgtggaaaca ttgccccagc ccaggaaacc gcgcgggatg agcgtgtcga
      781 agacggtgct caattggtcg cgcgtgttgc cggactcgtg cgtcacgaac gaattgcgca
      841 gatccagcac ctgcatgaga tcgccgtaca cccggtaagg gttgatcggc ttctcgtcgg
      901 aaaccatgac ctgccggtat ctgccgagcg cctcgtcgcg tgccgccttc acttccgccg
      961 caacgttacg cgccgccata tcgcccatag tctggccggc accggacgac gccagagcgc
     1021 tggtggtcgc ccggcgcaga ttagcctgga tcgccgcgtc caacgggatg accgccgcct
     1081 tggacggctc cgtgcggtcg gacacttcct ggcggagcgc ctgcaaggtc aggcgtgcat
     1141 cgccgatcac cgcgtacgcc gtggaataca tcttgttgat gtgcagctcg tcgatgttgc
     1201 agtggatgat agtcttcttc gccgcatcag gaatggcatg gctgaagcgg cccggcgaca
     1261 ggcttgagcc tatggcgagg atgaggtcgc ttttgttcag gtagtgtgcc acgtgatcgc
     1321 cgcgcacgcc gacaaacagc ttatggtttt ccggaaacgc ccctttggcc ttgagagtcg
     1381 tgatcaccgg cacgttcacc agctccgcca gcgctcgcag gtcttccgaa gcgcccgcgt
     1441 agatgacccc ctcgccggcg tagatcagcg ggttccgggc tgccagaagc tggtcaaccg
     1501 cggcgcgtac atccgccgga tccggcgctg atttccagcc cttcacggga cggtaggggt
     1561 cggcggtctc gtcatatgtc gccgtggcat tgggaatagc caggatcacc gggcccgggc
     1621 tgccgctacg cagcatggtg aaggcgcggc gcatgagctc aggtgtgcgc tcgggcttat
     1681 cgagatagcc gaaccacttc gacacggtct tcatcgccga tgcgacatcg tactggctgt
     1741 tgcccgagct gccggccggc acgccatcgg tgatgcacag aacgggcgag ccatcctcat
     1801 aggcctgcgc cagcccagca taggcgtact gcatgccggc ggcattgacg cccccttgga
     1861 aggtgcatac cccgatccgt gagttctccc cgggagatcc cccctcggag ccccccatga
     1921 tgcgcgagaa cgcgtcggcg accgcgacgg catagcggtc gtcgcgcatc atcagcatcg
     1981 gcatcccttc ccggccgaac gcattgttca cccggcagac agggaacgtc gaaacccaac
     2041 ccacaccttc ctgcttgaga atgcggctaa tgccggtggc tacatcgatc gtcatggacg
     2101 gggtctcctc tgcacggagc cgagccgtcg gcctccgtat gggcgcaccg aatgatcggc
     2161 cgcgtgccgt ttcccaactg ccgcggcggt gtgatcggcc tcgcaattgc gggcaattag
     2221 gaaaatgacc ggcaccggcc accctggcaa gagccggtcg ggcatgtgtg tcggacatga
     2281 gatcatcccg gacaagaacg gctgcgtaaa cgaggcaacg gctggcctta cgctcgggct
     2341 cttcgtgatg ccgtttcagg acgtcgcgtt cggctgactg ggacgcgacg gagcgtctcg
     2401 ccgcccctga acgccaagag atgggtgaca agcatggcga tcggcgcttg agccaaagcg
     2461 ggtcaatccc caatcaaaaa gaccctgatc gaaggcagga tgcacttcgt tgcgcttggg
     2521 cgctggccgc cgactggagc gacaagcttg tgagcacgac tcatgcgggt ctgtaagtta
     2581 agacgtagca atatcgaaat agcccgctgc cgtgctcggc tcgccgtgaa ccgcttgtcg
     2641 ttcagctacg ggaaaatcac cgtccgggtg cgcctgatca ggaacgcgtc gttgcgcagc
     2701 aaaaaatcag ccacgacttc cgcgtacgcc tcgggacgct cc
//
LOCUS       DASWQN010000133         4563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_542563, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000133 DASWQN010000000
VERSION     DASWQN010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542563"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1753
                     /locus_tag="VGC82_02190"
     CDS             <1..1753
                     /locus_tag="VGC82_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY0422114.1"
                     /translation="IARETGLRVVGPNCLGVVNALLDSRVTFMDITPIPKPERNAVGI
                     ISQSGALGMALAQGVVRGLSVSHVLTSGNSCDVDMADFVNYLATDPSCGSIACVFEGM
                     ATPERLLLAAENAWAADKPLVIFKMATGEQGAQAAMSHTGSLAGSHASYRAVFQRAGA
                     IVVDDFEALMETAAFFAKAPPPVARGAAVVAASGGAAIMATDRAEQHDVPMPQPTPDV
                     KAILETRIPEFGSSRNPCDVTAQVLADPESLGICAGALLSDPQYGVLVSPLTYGYAPS
                     AKRPQVYDALAKQSGKMAVVVWQTEWQDGPGVIEANQCDRVALFRSMNACFAALAAWH
                     WRADKRAAGAQVVPPTAPDVVARTKALVAEAGGRTLTERQAKEVLGHYGVPVVGEKLA
                     RSADDAVAAAAILGYPVVLKVESADLPHKTEAGVIRLNLRDENEVRAGYAAVMANAAK
                     VSPPPRINGVLVQPMVPQGIEMVVGARNDPLFGPLIVVGLGGILVEVLKDTALSPAPV
                     TPVQAEGLLRQLKGVKLLEGFRGMPAVDIGRLAQVISDVSRFAADHRDTVAELDVNPL
                     ICAGDRITAVDALIVVS"
     gene            complement(1842..2570)
                     /gene="lexA"
                     /locus_tag="VGC82_02195"
     CDS             complement(1842..2570)
                     /gene="lexA"
                     /locus_tag="VGC82_02195"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY0422115.1"
                     /translation="MLTRKQYELLVYIDRHLKQTGFSPSFEEMKDALSLKSKSGIHRL
                     ISALEERGYLARRHHRARALDVLRLPDNMVTRETPAQEAPADHETAHGFAPNVIRGDF
                     TQRLAGVRAASDAGAIQLPMYGRIAAGLPIEALRDTDSFIEVPMALLGAGEHYGLQVA
                     GDSMIEAGILEDDTVIILKGDTAENGQIVVALVDDTEVTLKRLRRRGNSIALEPCNVR
                     HETRIFPADRVKVQGRIVGLMRRY"
     gene            complement(2714..3919)
                     /gene="glp"
                     /locus_tag="VGC82_02200"
     CDS             complement(2714..3919)
                     /gene="glp"
                     /locus_tag="VGC82_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY0422116.1"
                     /translation="MISVEEARLRILHTLRPTPAEIVALADAWNRVTAQPVAARLTQP
                     PVDVSAMDGYALRAADGTLKARLRVAGAAPAGHPFDGVVRAGETVRLFTGSVVPAGAD
                     AILLQEDATRDGDGVLVNEAVIAGRHIRRAGQDFVIGDVVLPPGKRITARDVGLAAAA
                     NHPWLAVHRRPRVAILATGDEIAMPGEPIPPGGIVSSNSHALAALVRAAGGEPMVLPI
                     AVDDHDAIGAVADSVAGMDILITTGGASVGDHDLVIEALKARGMTLDFWQIAMRPGKP
                     LLFGQLGAMPVLGLPGNPVSAMVCGILFLLPALLRLSGLPAAPPPVSAAILGAAVKAN
                     DHRADHLRASVSNDETGRIVVMPFPVQDSAMLRRLAHADALILRAPLAPALPEGAEVS
                     IIRLDSLGL"
     gene            4168..4380
                     /locus_tag="VGC82_02205"
     CDS             4168..4380
                     /locus_tag="VGC82_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HEY0422117.1"
                     /translation="MADHVYKIVELVGSSEESVTKAIDRAISKAAVTLRHLGWFEVVQ
                     VRGQIDNGKVAQYQVTIKAGFRLEDE"
BASE COUNT          787 a         1450 c         1528 g          798 t
ORIGIN      
        1 gatcgcgcgg gaaacagggt tgcgcgttgt cggacccaat tgcctcggcg tggtcaacgc
       61 gctgctcgac agccgcgtga cgttcatgga catcacgcca atccccaaac cggagcggaa
      121 cgcggtcggc atcatcagcc agtccggcgc gctgggcatg gctctggcgc agggcgtggt
      181 gcggggcctg tcggtcagcc acgtgctgac ttcgggcaat tcgtgcgacg tggacatggc
      241 ggacttcgtc aactacctgg cgaccgaccc gtcctgcgga tcgatcgcct gcgttttcga
      301 aggcatggcg acaccggagc ggttgctgct ggccgcggaa aatgcctggg ccgccgacaa
      361 gccgctggtt attttcaaga tggcgaccgg cgagcagggc gcccaggcgg cgatgtccca
      421 caccggctcg ctggccggtt cgcatgcatc ctatcgggcg gtgttccagc gggcgggtgc
      481 gatcgtggtg gacgatttcg aggcgctgat ggaaaccgct gcgttcttcg ccaaggcgcc
      541 gccgccggtg gctcgtggtg ccgccgtcgt ggccgcttcc ggcggggcag cgatcatggc
      601 gacggaccgc gcggagcagc acgacgtgcc gatgccgcag ccgacgccgg acgtcaaagc
      661 gatcctggag acgcgcattc cggagttcgg atcctcgcgc aatccatgcg acgtcaccgc
      721 ccaggttctc gccgatcccg agtcactggg gatctgcgcc ggcgcattgc tgagtgatcc
      781 gcagtacggc gtgctggtct cgccgctgac ctatggctac gcaccgtcgg caaagcggcc
      841 gcaagtgtat gacgccctgg cgaagcagtc gggcaagatg gccgttgtcg tctggcagac
      901 cgaatggcag gacgggccgg gcgtgatcga ggcgaaccag tgcgaccggg tggcactgtt
      961 ccgctccatg aacgcctgtt tcgcggcttt ggcggcttgg cattggcgcg cggacaaacg
     1021 tgcggcgggt gctcaggtcg ttccacccac cgcgcccgat gtcgtcgcgc gaacaaaggc
     1081 gcttgtcgcc gaggcgggcg ggcggacgtt gaccgagcgg caggcgaagg aggtactggg
     1141 acattacggc gttccggtgg ttggcgaaaa gctggctcgg tcggcggacg acgcggtcgc
     1201 tgccgccgcg atcctgggct atccggtggt gctgaaggtc gaatccgccg acctgccgca
     1261 caagaccgag gcgggagtga tccgcttgaa cctgcgcgac gaaaacgagg tgcgggccgg
     1321 ttatgccgcg gtgatggcga acgccgccaa ggtgtcgccg ccgccgcgga tcaatggcgt
     1381 cctggtacag ccgatggtgc cgcaggggat cgagatggtt gtcggcgccc gcaacgatcc
     1441 gctgttcggt ccgctgatcg tggtcgggtt gggcggcatc ctggtggagg tcctgaagga
     1501 taccgcgctg tcccccgccc cggtgacccc ggtgcaagcc gagggcctgt tgcggcagtt
     1561 gaagggtgta aagctactgg agggcttccg cgggatgccg gctgtggaca ttgggcggtt
     1621 ggcccaggtt atcagcgacg tgtcccgctt cgccgccgat caccgcgaca cggtggcgga
     1681 gcttgacgtg aacccgctga tctgcgcggg agaccgcatc acggcggtgg atgcgctgat
     1741 tgttgtttct taatcagtag agggggtttc ggcgggacga attgacatga cggctggaac
     1801 attgtcgcgc ggaatgagcc ggacgactgg cgcagccggt ctcagtacct ccgcatcagg
     1861 ccgacaatcc ggccctgtac cttcacccgg tcggccggga agatccgagt ctcgtggcgg
     1921 acattgcagg gctccagcgc gatcgagttg ccacgccggc gcaggcgctt cagcgtgacc
     1981 tcggtgtcgt ccaccagtgc caccacgatc tgcccgttct ccgcggtatc gcccttcagg
     2041 atgatcaccg tatcgtcttc caggatccca gcctcgatca tcgagtcgcc ggcgacctgc
     2101 agcccgtaat gttcgccggc accgagcaga gccatcggca cttcgataaa gctgtcggtg
     2161 tcgcgcagcg cctcgatcgg caggcccgcg gcgatgcggc catacatcgg aagctggatg
     2221 gcgcccgcat cgctggcggc ccggacgccg gcgagacgct gcgtgaagtc gccgcgaatg
     2281 acattgggag cgaacccatg cgccgtttcg tggtcggcgg gtgcttcctg cgccggcgtt
     2341 tcccgcgtca ccatgttgtc cggcaagcgt aggacatcca gcgcacgcgc ccggtgatgc
     2401 cgccgcgcca gatagccgcg ttcctcgagc gcggaaatca accggtgaat accggatttc
     2461 gacttcaggc taagcgcgtc cttcatctcc tcgaagcttg gggagaagcc ggtctgcttc
     2521 aggtgccgat cgatataaac cagcagttca tattgcttgc gagtcaacat ggcggacttc
     2581 cttgaatgac cgccggaaca aaccggcaac cggccccggc tgttctatgt tggttccagg
     2641 atcttcgtca agcgaaactg tgtagaagat gaaaaacccg cgcctgtccg cgaatttatt
     2701 cgaactgatg tcgttacaat cccagggaat cgaggcgaat gatgctgacc tcggccccct
     2761 ctggcagcgc cggcgccagt ggcgcgcgca ggatcagcgc gtcggcgtgt gccagtcggc
     2821 gcaacatcgc cgagtcctgc accggaaacg gcatcacgac gatacggccc gtttcatcgt
     2881 tgctgaccga cgccctcagg tgatccgcgc ggtgatcgtt cgccttgacc gccgccccca
     2941 gaatggcggc ggacaccggt ggcggtgcag cgggcagccc ggatagacgc aacagcgccg
     3001 gcaacaggaa aaggatcccg caaaccatgg ccgagaccgg attgcccggc agccccagca
     3061 ccggcatggc gcctagctgc ccgaacaaca acggctttcc cggccgcatc gcgatctgcc
     3121 aaaagtccag ggtcatgccc cgggctttca gcgcctcgat gaccaggtcg tggtcaccga
     3181 ccgaggcacc gcccgtggtg atcaggatat ccatgcccgc caccgagtcc gcgaccgccc
     3241 cgatcgcgtc atgatcgtca acggctattg gcaggaccat cggctcgcct cccgcggctc
     3301 gcaccagggc ggccagcgca tgcgagttcg aactgacgat gccgccgggt gggatcggtt
     3361 cgcccggcat ggcgatttcg tcgccggttg ccaggatagc gacccgcggc cggcgatgca
     3421 ccgcgagcca gggatgattc gcggcagccg ccagtccgac atcgcgggcg gtgatccgct
     3481 ttcctggcgg caggaccacg tctccgatga cgaaatcctg accggcacgg cggatatgcc
     3541 ggccagcgat aacggcctcg ttcaccagca ccccatcgcc atctcttgtg gcgtcctcct
     3601 gcaacaggat tgcatcggcg ccagctggaa caacgctgcc ggtaaacagc cgcacggtct
     3661 cgcccgccct cacgacgcca tcgaacggat gcccagcggg cgccgcaccg gctacgcgaa
     3721 gccgagcctt cagcgtgcca tccgccgcac gcagcgcata tccgtccatg gcggacacat
     3781 caaccggagg ctgcgtcagc cgagcggcca cgggctgcgc ggtgacgcgg ttccacgcat
     3841 cggcgagggc gacgatctcg gcgggcgtgg ggcgcagcgt gtgcaagatg cgcaagcggg
     3901 cttcctcgac actgatcata cccgcgctcc attggttgcg acgtgcgtgt ggctgcgacg
     3961 tgcgtgtggc tgcgacgtgc ggggcatttt gtgcgatggt gacgccgccc tcaagccggg
     4021 tctgccggaa caggcggttc tggacgaccg ggcggggcac gctcgcgatt tcgtgcccac
     4081 catgccttgc tgggctgccc taacattggc gaggaacccg ggaataaacc cgacggagca
     4141 acatcgatct tgtagggagc gtctccaatg gccgaccatg tttacaaaat tgtcgaactt
     4201 gttggttcat ccgaagaaag cgtcacaaag gcgatcgacc gggcaatttc gaaagccgcg
     4261 gtcacactac ggcatctggg gtggtttgag gtggtgcagg tccgtggcca gatcgacaat
     4321 ggaaaagtgg cgcagtatca ggtcacgatc aaagcaggct tcaggttgga agatgaataa
     4381 caccgccttt gcctgagatg cggtgacact cctgcgtcgg tagcggatac gcattcggcg
     4441 gttggcttgc gcgcatccgt acgatgggcg cacaactgcc gttgccggct tccccgcctc
     4501 gttgctggcc aaacgccttc ggcctatcct ggtctctgcc tgtaatggct taaggacccc
     4561 ggc
//
LOCUS       DASWQN010000134         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1862432, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000134 DASWQN010000000
VERSION     DASWQN010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862432"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..656)
                     /locus_tag="VGC82_02210"
     CDS             complement(312..656)
                     /locus_tag="VGC82_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0422118.1"
                     /translation="MAHPPMTLDEIRASRPAVDRTRIAATTEADIRRNKIEDGEDPDA
                     PLPPFRPIPNVRAIRTKLRMTQEAFANAIGVPVATVRNWEQSRTSMDPAVRSLLLVVE
                     REPVAALRALAE"
     gene            complement(638..862)
                     /locus_tag="VGC82_02215"
                     /pseudo
     CDS             complement(638..862)
                     /locus_tag="VGC82_02215"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
     gene            complement(971..1288)
                     /locus_tag="VGC82_02220"
     CDS             complement(971..1288)
                     /locus_tag="VGC82_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422119.1"
                     /translation="MMRFTAVAALFPDLRETELHAWVERGWVRPAGAAPDWIFQEIDI
                     ARVRLIHDFRHAMAVPEETMPLVLSLLDQVYTLRGQMRAVARAVEGQPEAVRAAILAA
                     LRE"
     gene            complement(1285..2178)
                     /locus_tag="VGC82_02225"
     CDS             complement(1285..2178)
                     /locus_tag="VGC82_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY0422120.1"
                     /translation="MKDPYEILGVQRTADEAAIRAAYRKLAKTHHPDVNPGKPDAATR
                     FGEISSAYDLLSDKDKRARFDRGEIDAEGHEVYQQRQYYRDTPGTENYQSAGGFSQED
                     LEAFFAQQFGQAGRRSDWGATGRVRPGRDMQYSLTVSFVDAASGTTRRLSLPDGKTLD
                     VRIPPGTEDGHVLRLRGQGGPGYNGGPAGDALIEVTVAPDPRFHRDGDDIITDVPVSL
                     SEAVLGTSLEVPTIHGPVRLSIPPGSGTGTRMRLRGKGIRQGHQFVQLQVVVPPGDEP
                     ELAEFLKTWVPKASFNPRDKS"
     gene            complement(2214..2990)
                     /locus_tag="VGC82_02230"
     CDS             complement(2214..2990)
                     /locus_tag="VGC82_02230"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY0422121.1"
                     /translation="MNEILLRHDNGGIATLTLNRPSARNALSMDLMETLDAELSGIAQ
                     DRTVKVVIIAGNGPAFCAGHDLREMRASPTRSAYEQVFALCSRLMQRIVRLPKPVIAR
                     VHGVATAAGCQLVASADLAVAADTARFATPGVDIGLFCSTPMVALTRSIGRKAAMEML
                     LTGDMITAARARELGLVNRVVPEALLEGATLALAEQIAAKSPLTVALGKEAFYRQAEM
                     DLSAAYDHAAQVMTRNMLARDAGEGIDAFLAKRQPQWTGT"
     gene            3167..>3291
                     /locus_tag="VGC82_02235"
     CDS             3167..>3291
                     /locus_tag="VGC82_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY0422122.1"
                     /translation="MPSNTAYPRGELSLRTVAMPADTNPAGDIFGGWIMSLMDLAA"
BASE COUNT          569 a         1030 c         1097 g          595 t
ORIGIN      
        1 cagtgaagtt cgacgttgac tgcgatgcgg atcccgccaa gctggagcgg atgattcaaa
       61 ccaccgaacg ttactgcgtt atcctgcaga cgctgaagtc gccgccgaaa ctggcgatgg
      121 agatcgcctg agtcagggat gatcgagccg agcggaatgt cgcagaaccg cttggatcga
      181 acgttgacga tcgtacggtt catggagagt cgcgccgtca acaacggagg atccaacacc
      241 gcctgccttt ccaagaccac atcctcgctc gtttgcgagt aattgtggcg gtgtccagga
      301 cggcgctggg attactctgc cagcgctcga agtgcggcga ccggctcgcg ttccaccaca
      361 agcagcaggg accggacagc cgggtccatg ctggtgcggg actgctccca atttcgcacc
      421 gtcgccacgg gcacgccgat cgcattggca aaggcttcct gcgtcatccg aagcttcgtc
      481 cggatcgccc gtacgttcgg aatcggacgg aacggtggaa gtggagcgtc gggatcctcg
      541 ccatcctcga ttttattccg gcggatgtct gcctcggtgg tagccgcgat cctggtgcgg
      601 tccacggccg gccggctcgc cctgatctcg tccaatgtca ttggtggatg tgccactggt
      661 gccgttcctt cctgtccgcc agtcgggccg agatgatgcg gcgaacatca ccccaaacgg
      721 taaatacgac atggagcacg atgccgtcgg tctcttcaat cgttcgcaca cgggcttcgt
      781 tgtcgtcccg ccggctgtcc cgccactgga tcgtgtcgcc ctcgaaaatc aacgcggcga
      841 agccgaagcc aaatccccgc tcccgccgat tccgctagtc cttcgcggca tcccacttga
      901 agcgcatggg caaatctacg ctaacagcgc agcagcgtaa acgagaatcc gaaccaacaa
      961 ccggcgaaca ctattcccgc agtgccgcca ggatcgccgc gcgaactgcc tccggctggc
     1021 cctctaccgc gcgggcgacg gcacgcatct ggccgcgcag cgtatagacc tgatcgagca
     1081 gcgacagcac cagcggcatc gtttcctcgg gcactgccat cgcatggcgg aagtcgtgga
     1141 tcagccgcac ccgagcgatg tcgatctcct ggaatatcca gtcgggggcg gcacctgcgg
     1201 ggcggaccca gccgcgttcc acccaggcgt gcaactcggt ctcgcgcagg tcggggaaca
     1261 atgccgcgac ggcggtgaag cgcatcatga cttgtctcgc gggttgaagc tcgcctttgg
     1321 cacccacgtc ttcaggaact ccgccagttc cggctcatcg cccggaggca ccacgacctg
     1381 gagctggacg aactgatgcc cctggcggat ccccttgccg cgcagccgca tccgggttcc
     1441 ggtgccggaa ccggggggga tcgacaggcg gaccgggcca tggatcgttg gcacctccag
     1501 cgacgtgccc aggaccgctt cggacaacga aaccggcaca tcggtgatga tgtcgtcgcc
     1561 gtcacgatgg aaccgcgggt cgggggcgac ggtgacctcg atcagcgcat cccctgccgg
     1621 gccgccgttg tatcccgggc cgccctggcc acgcaggcgc agcacgtggc cgtcctcggt
     1681 gcccggcggg atgcggacgt ccagcgtctt gccgtccggc aacgaaagcc gccgcgtcgt
     1741 gccacttgcg gcgtcgacga agctgacggt cagagagtac tgcatgtcgc ggcctggtct
     1801 gacgcggccg gttgcgcccc aatcagagcg tcggccggcc tggccgaact gctgggcgaa
     1861 gaatgcttcc agatcttcct ggctaaagcc gccggccgac tgatagtttt cggtgccggg
     1921 ggtgtcgcgg taatactggc gttgctgata aacctcgtgg ccctcggcgt cgatctcgcc
     1981 gcgatcgaag cgggcacgct tgtccttgtc ggacagcagg tcgtaggcgg agctgatctc
     2041 gccaaagcgc gttgcagcgt ccggtttgcc cgggttgacg tcgggatggt gcgtcttcgc
     2101 cagcttgcgg taggcggccc ggatggctgc ctcatccgcg gtgcgctgga cgccaaggat
     2161 ctcgtacggg tctttcatta cagttggtca ggtagcgatg aggacatgca cgatcaagtc
     2221 ccggtccatt gcggttggcg ctttgccagg aacgcgtcga tgccttcgcc ggcgtcacgg
     2281 gccagcatgt tccgggtcat cacctgcgcg gcgtggtcgt aggcggcgga aaggtccatc
     2341 tcagcctggc ggtagaaggc ttccttgccc agcgccacgg tcagcgggct tttcgccgcg
     2401 atctgttcgg ccaacgcgag ggtcgcgcct tccagtaggg cctctggcac gacgcggttg
     2461 accaggccga gttcgcgggc gcgggctgcg gtaatcatgt cgccggtcag cagcatctcc
     2521 atcgccgcct tgcggccgat cgaacgggtc agggcgacca tgggggtgga gcagaacaaa
     2581 ccgatatcga cgcctggcgt ggcgaaccgc gcggtgtcgg cggcaacggc caggtcggcg
     2641 gaggcgacca actggcaacc cgcggcggtg gcgacgccat ggacccgggc gatcacgggc
     2701 ttgggcaatc ggacgatgcg ctgcatcagg cgtgagcaaa gggcgaacac ctgctcgtag
     2761 gcggatcgtg tgggggaagc tcgcatttcg cgtagatcat ggccggcgca gaaggcggga
     2821 ccgttgccgg ctataatgac gactttgaca gtgcgatcct gggcgatgcc ggatagttcg
     2881 gcgtccagcg tctccatcag gtccatcgac agggcgttac gggcggacgg gcggttcagc
     2941 gtcagggtgg cgatgccacc gttgtcgtgg cggagcagga tttcgttcat ggtctggagt
     3001 ttagaccagg atcggatggg gttgaagcgg cctcggcgta tgctgggttc cgccgatagg
     3061 gttcgccggg ccgatcaagc tgccatcaac gccgggtgcg gccgtatgaa gcctaaatgg
     3121 cgatatggac gttgtagcgt ggcacaacca aacgaaggag cctgccatgc cgtcgaacac
     3181 cgcttatccg cgcggcgagc tgtcgttgcg aaccgtcgcg atgccggccg ataccaatcc
     3241 ggccggggat atattcggtg gctggatcat gtccctgatg gacctcgcgg c
//
LOCUS       DASWQN010000135         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_674539, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000135 DASWQN010000000
VERSION     DASWQN010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674539"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..908)
                     /locus_tag="VGC82_02240"
                     /pseudo
     CDS             complement(256..908)
                     /locus_tag="VGC82_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612315.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            complement(920..1780)
                     /locus_tag="VGC82_02245"
     CDS             complement(920..1780)
                     /locus_tag="VGC82_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0422123.1"
                     /translation="MSPLRRRMIDDMQIRNLTPNTQRVYVAQVVRFACHFRKSPGLLG
                     PAEIRTYLIHLTQERRLAASSIIVAVSALRFFFTVTLKRPWIVEDDIPAGRQAKKLPV
                     VLSQEEVARFLGAVDNQKHRVILTVCYATGLRISEAVRLKPGAIDSKRMVIRVEQGKG
                     RKDRYVMLPPKLLDILRDYWRRTHPGEWLFPGRYPGQPISPLSIDRTCREVRLLCGID
                     KPVAPHSLRHAFAVHLLEAGTDLRTIQLLLGHRNLSTTAQYLMIATSTVCATASPLES
                     LPTVPDLVPA"
     gene            complement(1949..2088)
                     /locus_tag="VGC82_02250"
                     /pseudo
     CDS             complement(1949..2088)
                     /locus_tag="VGC82_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611353.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          443 a          626 c          664 g          355 t
ORIGIN      
        1 gcgtgatcgt caaggcgtgg tccgatgggc ccgcttcaat gaactcctgg cacggcaccc
       61 actgccttca gtcaagatct tccatggcta cgccgccgcg agcgaatctc tctcgtgaag
      121 aaccggatgc gggaaatctg cacgtccggg tctgtgaggg atgggggcgg aaacgtcccc
      181 atctactcgg cactcggctc gctatgccgg tgtcaggtag cgcctgaaca tcgcgggctc
      241 atgcaggtga cgatgttcgg agaagaagtt cagccgccgg cggtaaacgc cttctccagg
      301 tagcgcagga atagcccccg aaacatccga gacagcaccc ggaccggcag gaagaacccc
      361 ggtcggcagg cgatccagct ttgcccgtcc aacgcgatcc cgccgccggg aacaagaaaa
      421 tgcagatgcg gatggtggtt gagcgcttgt ccccaggtgt gcagcacacc gaggaaaccg
      481 atctccgcac ccaaatgctc gggatcagcg gcgatgctgc gcaaggtctc cgacgcagcc
      541 cggaacagga tatcgtacac cacggtctga ttctggaacg cgatgacctc aatctgcgtc
      601 ggcacggtga acacgacgtg gaaatagggc acgtcgagca gttccgcctg acgatcctcc
      661 agccattgcg cccgcgccag accctggcat ttagggcaat tgcgattgcg acacgagttg
      721 aaggagacgc gctgatggcc gcaatcgccg cagcgctcga catgaccacc gagcgcagcg
      781 gtgcggcaac tctcgatcgc ggccatggca tgccgctgtg ctgacgacag cgatccacca
      841 tgctgatcac ggaaagcagg gccgaagtgg cggaacacat ccgccacttc cagcccctcg
      901 cgagccacga acccccgcgt caggcgggta ccaagtccgg cacagttggc aaagactcca
      961 gcggactggc ggtagcgcag accgtgctgg tggcgatcat gagatactgg gcggtggtgc
     1021 tcagattgcg atggccgagc agcaactgga tggtgcggag atcggtgcct gcttccagca
     1081 gatgaacggc gaaggcatgg cgcagtgaat gaggggcgac cggtttgtcg atgccgcaca
     1141 gcagcctcac ctcccggcag gtgcgatcga tggacagcgg agaaatcggc tggccgggat
     1201 agcgacccgg aaacagccac tctccgggat gggtgcgcct ccaatagtcc ctcaggatgt
     1261 cgagaagctt gggcggcagc atgacgtagc ggtctttccg gcctttaccc tgttcgacac
     1321 ggataaccat gcgcttgctg tcgatcgcgc cgggcttgag gcggaccgct tcggagatcc
     1381 gcagaccggt cgcgtaacag acggtcagga tcacccggtg cttttgattg tcgacggcgc
     1441 cgaggaaacg cgcgacttct tcctggctca gcacaacggg aagcttcttc gcctgacggc
     1501 cggcggggat gtcgtcctcg acaatccagg gccgcttgag cgtgacggtg aagaagaacc
     1561 gcagcgcgga aaccgcgacg atgatcgaac tggcggcaag gcgcctttcc tgcgtcaggt
     1621 ggatcagata cgtccgaatc tcggctggcc cgagcagtcc gggcgacttg cgaaagtggc
     1681 aggcaaaccg gacgacctgc gcgacataca cgcgctgcgt gttcggggtc aggttgcgga
     1741 tctgcatatc gtcgatcatg cgacggcgaa gcgggctcat tgacaactcc tttgcgagat
     1801 ggacagcgcc acatggcgcc agagcatcgt ctcgcagttg agttgtcaga ccagacagga
     1861 cgtgttccgc agcttacgca aacaaagcca aatagcatta gaggaacgag gtctaagtca
     1921 cagaactacc gcgaagcggt ttagtgcaac ggcctttgcc gtccatgctg atgcggatac
     1981 cgctgcttgc cagcacgccg gtgaacgcct cgctggtgaa ctgggcgccc tgatcggtgt
     2041 tgaaaatatc aggcgaaccg taacgatcca gcgcgtcctg caaggcct
//
LOCUS       DASWQN010000136         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_806992, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000136 DASWQN010000000
VERSION     DASWQN010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806992"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VGC82_02255"
     CDS             <1..198
                     /locus_tag="VGC82_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEY0422124.1"
                     /translation="HLPVQLLDDRIRIRADHVIGEMIEGLGGHVDLISAPFDPEAGAY
                     SGGGHHHHHHHHADDDDHPVA"
     gene            137..934
                     /locus_tag="VGC82_02260"
     CDS             137..934
                     /locus_tag="VGC82_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HEY0422125.1"
                     /translation="MAGIIIIIITMPMTMTILSPEVTRHEATNTRKPFVPSCLGGGEA
                     RPDDALTPPALLRLMAWLSPAFPTGAFAYSHGLEWAVEAGDVTNETYLLAWLCDVIAD
                     GSGRTDTILLRHAHRSAGNPGALTEVANLACATSPCRERQSESLDQGMAFLLAAAAWG
                     TPATPERMPYPVAVGAVAGVHRIPEDAAAAAYLQATVTNLISAAVRLVPLGQTAGLRV
                     LAALEPAILGVAAKTKDATLDDLGGCTFRSDLAAMRHETQYTRLFRS"
     gene            931..1560
                     /gene="ureG"
                     /locus_tag="VGC82_02265"
     CDS             931..1560
                     /gene="ureG"
                     /locus_tag="VGC82_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEY0422126.1"
                     /translation="MTISPHGPLRVGIGGPVGTGKTALMDALCKHFRDRYDIAAITND
                     IYTKEDAEFLTRAGSLPVERIMGIETGGCPHTAIREDASINLAGVADLRKRFPKLDLI
                     LIESGGDNLAATFSPELADLTIYVIDVSAGDKIPRKGGPGIIRSDLLVINKIDLAPYV
                     GASLDVMDRDAKKMRGGRPFVFANIRAGTGVAEIAAFVEKQGGLRPVRA"
BASE COUNT          300 a          515 c          519 g          323 t
ORIGIN      
        1 cacctgccgg tgcaattgct ggacgatcgc atccgcatcc gcgcggatca tgtgatcggc
       61 gagatgatcg aggggctggg cggacatgtg gatctgatca gcgcaccctt cgaccctgaa
      121 gccggtgcct attcgggtgg cgggcatcat catcatcatc atcaccatgc cgatgacgat
      181 gaccatcctg tcgcctgaag tgacccgcca cgaagccacc aacactcgaa aaccctttgt
      241 gccttcgtgt cttggtggtg gggaagctcg gcctgacgac gcactgactc cacccgcttt
      301 actccggctg atggcgtggc tgtcaccggc ttttccgacc ggcgcattcg cctattcgca
      361 cggcctggaa tgggcggtgg aggccggtga cgttacgaat gaaacctatc ttctggcctg
      421 gctgtgcgat gtcatcgctg atggttcggg gcgtaccgac acgatcctgc tccggcacgc
      481 acaccgctct gccggcaatc cgggcgcgct gacggaggtc gcgaaccttg cctgcgcgac
      541 ctccccgtgc cgtgagcggc aaagcgaatc cctcgatcag ggcatggcct ttctgttggc
      601 ggctgccgcg tggggaacac cggcaacgcc ggagcgcatg ccatatccag ttgccgttgg
      661 cgcggtcgcc ggcgtgcatc gcatcccgga ggacgctgcc gccgcggctt accttcaggc
      721 gaccgtcacc aacctgatat cggccgcggt tcgtcttgtg ccgcttggcc agacggctgg
      781 gctgcgtgtg ctggctgcac tggaaccggc gatactgggc gttgccgcga agaccaagga
      841 cgccactctc gacgatcttg gcgggtgcac cttccggtcc gatcttgctg ccatgcggca
      901 tgaaacccag tacacgaggt tgttccgttc atgaccatct ccccccacgg cccgttgcgc
      961 gttggtatcg gcggccccgt cggcaccggc aagaccgcgc tcatggacgc gttgtgcaag
     1021 cacttccgtg accggtacga catcgcagcg atcaccaacg atatctatac caaggaagat
     1081 gctgagttcc tgactcgagc cggctcctta ccggtcgagc gtatcatggg catcgaaacc
     1141 ggcggctgtc cgcataccgc gatccgcgag gacgcgtcca tcaatctggc tggggtcgcg
     1201 gatctgcgga agcggtttcc aaagctcgat ctgatcctga ttgagtcagg gggcgataat
     1261 cttgccgcca ctttcagtcc ggagctggcg gacctgacga tttacgtcat cgacgtgtcg
     1321 gccggggaca agattccgcg caagggcggt ccgggcatca taagaagcga tctgctcgtc
     1381 atcaacaaga tcgaccttgc gccttatgtc ggtgccagtc ttgacgtcat ggaccgcgac
     1441 gcgaagaaga tgcggggtgg gcgaccgttc gtgttcgcca atatccgggc cggcacgggc
     1501 gtcgccgaga ttgcggcatt cgtggaaaag caaggcggac tccgtccagt tagagcatga
     1561 tcacaccggg cggaacgtac ggagcgtgtg aagcgtacga tgaaacaaag ggctcgagca
     1621 ttcgcagccc gtacagtcag gcggacacgc tctggct
//
LOCUS       DASWQN010000137         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2390095, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000137 DASWQN010000000
VERSION     DASWQN010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2390095"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="VGC82_02270"
     CDS             complement(<1..1491)
                     /locus_tag="VGC82_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0422127.1"
                     /translation="MSLRRPYTSAIAALLVVLLGVLSLTGMIVDIFPVIDIPVVLVAW
                     NYPGLTTEDMERRVVFISERAYSTTVNGISRIESQSIPGIGILKVYFQPGTEIGGAIA
                     QITSVNNSILRSAPPGMQPPAVIQFNASNVPVVQLTMSSKTLTEQQIYDYSLNFIRVK
                     LFTIPGLSTPGPFGGKQRQMNVDVDPGLLAAKGFSLTDVVNALQASNVIVPAGTARIG
                     DREYNVQLNSSPAAVERFNDLPIGVFDGRPVTIGDVGRVTDSFANQGNIVHVDGKRAV
                     YLAILKHADASTLAVVDATRSALPEVQAAAPAGLELKLDFDQSVFVRGAVENVVREAI
                     ISSILVSVLILVFLGSWRNTVIVSASIPLSVFAGIIGLFLTGNSINLMTLGGLALAIG
                     LLVDNATVVIENIHRNLMLGKKLTVAILDGSAEVIQPLTVATLAICIVFFPVVMLFGV
                     ARYLFIPLAATVVFCMLASYALSFTVVPSFARLLLASDKTHHAPPKG"
BASE COUNT          375 a          500 c          484 g          274 t
ORIGIN      
        1 gcctttcggc ggagcatgat gcgtcttgtc cgacgccagc agcagccgag caaaactcgg
       61 caccacagta aagctcagtg cataggacgc gagcatgcag aatacgacgg tggcggccag
      121 cgggatgaac aggtagcggg ccacgccgaa cagcatcacc actgggaaga acacgatgca
      181 gatcgccagg gtagcgaccg tcagcggctg aatcacctcg gcggagccat ccaggatggc
      241 gaccgtcagc ttcttgccca gcatcaaatt ccgatggatg ttctcgataa cgacggtcgc
      301 gttgtccacc agcaggccga tcgccagcgc caggccgccc aaggtcatca ggttgatgct
      361 gttgccggtc aggaacagtc cgatgatgcc ggcgaacacc gacaacggga tggatgccga
      421 cacgatgacc gtgttgcgcc agcttcccag gaacaccagg atcagcaccg aaaccaggat
      481 ggaggagata atcgcctccc gcaccacgtt ctccactgcg ccccggacga agaccgactg
      541 gtcgaaatcg agcttcagct ccagtccggc aggtgccgcg gcctgcacct cgggcagtgc
      601 cgaacgggtc gcgtccacca ccgcgagggt cgaggcgtcg gcgtgcttca agatcgccag
      661 atagaccgcg cgcttgccgt cgacatgcac gatgttgccc tggttggcga agctgtcggt
      721 aacgcgcccg acatcgccta tggtgaccgg acgcccgtcg aaaacgccga tcggcaggtc
      781 gttgaagcgt tccactgccg ctgggctgga attgagctga acattatact cgcgatcgcc
      841 gatccgggcg gtacccgcgg gaacgataac gttcgacgcc tgcagtgcat tgaccacatc
      901 ggtaagcgaa aagcctttcg ccgcgagcag gcccggatcc acatcgacat tcatctgccg
      961 ttgcttgccc ccgaacggcc ccggcgtgga aagacccgga atggtgaaca gcttcacccg
     1021 gataaaattc aaactatagt cataaatctg ctgctcggtc agtgtcttag acgacatcgt
     1081 caactgcacg accggcacgt tggaggcgtt gaactggatc accgccggcg gctgcattcc
     1141 aggcggtgcg ctacgcagga tcgagttgtt caccgacgtg atctgcgcga tggcgccgcc
     1201 gatttcggtt cccggctgga aatacacctt caggatgccg atgccaggaa tcgattgcga
     1261 ttcgatgcgc gagatgccgt tgacggtggt ggaataggct cgctcgctga tgaagacgac
     1321 acgccgttcc atgtcctcgg tggtcaagcc aggatagttc caagcaacca ggacgacggg
     1381 aatatcgatg accggaaaga tgtcgacgat catccccgta agcgacagca cgcccagaag
     1441 aacaacgagt aaagccgcga tcgcggacgt gtaaggccgg cgcaacgaca tacgcaccag
     1501 accaatcatt aattaccccc cggtagcagc caagcctgcg tcaccaaagg atagtattgc
     1561 cgggagataa cgccttgcca agcgccacgg gcgacagcaa acggcaacgc ttccgatcat
     1621 tgtcggcccc att
//
LOCUS       DASWQN010000138         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2522227, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000138 DASWQN010000000
VERSION     DASWQN010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2522227"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1475
                     /locus_tag="VGC82_02275"
     CDS             216..1475
                     /locus_tag="VGC82_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422128.1"
                     /translation="MGETEVEQTVSRRSVVGLGLAVGLVTAVPRIARAADQTIRIGVL
                     TDMAGTYASQTGPGSVLGAQLAIDDFRKQHPEIRVELLAADMQLKPDVALAIASDWYD
                     NKGVDVLTDLPLSSAAIGVGEMAKTKDKLAIYTGAASSILTGERCGPNHLHWSYDTWG
                     NPHAVVDATVREGGDTWFFITADYAYGQQLARDSASFVVAGGGKVLGEIRHPFPGTTD
                     FSSFLLAAKASGAKVIAFANGGADSDNCIKQAAEFGLVAGGIKVVGVGLLVNSLPSIG
                     LTAAQGVRFSEPYYWDLNDDTRAFGRRYQAQIKGASMPNSAQAGQYSAVSHYLKAVVA
                     MGPEKAKASGRATVAQMKMIPIQDQIFGKSEIREDGRVIHAMYLFEAKAPNESKEQWD
                     VVKVKSTIPMEKAFRPINEGKCSMIRT"
BASE COUNT          325 a          414 c          489 g          293 t
ORIGIN      
        1 actatgggtt tcaaactgga cgcggtttgt gacgccccag acggcagcgg ccacgcaatc
       61 atctgcattc gaacatcgcc ggattgcgct attcaggcga aggatcggct ggcgaggtag
      121 agcggagcga actgtctcgc gctggttgtc accgacggcg cgacgggcca gactgatgtc
      181 acgacaacgg atgacccagg aaacgggata tcgtcatggg ggaaacggaa gtggagcaga
      241 ccgtcagccg tcgttcagtg gttggtttag gacttgccgt gggccttgtg acggcggtgc
      301 cgcggatcgc gcgagcggcc gaccagacta tccgtattgg cgtcctgacc gacatggcgg
      361 gcacctatgc gtcgcagacc ggccctgggt cggttctcgg tgcacaactc gcgattgatg
      421 atttccggaa gcagcatcct gagatcaggg ttgaattgct tgccgcagac atgcagttga
      481 agccagatgt ggcgctagca atcgccagcg attggtacga caacaagggc gtcgacgtgc
      541 tgactgacct accgctgtcc tccgccgcaa ttggcgtagg agaaatggcc aaaacgaagg
      601 acaagctggc gatttatacc ggcgctgcgt cctcgatcct gactggcgaa cgttgcgggc
      661 ccaaccatct gcactggtca tacgacacct ggggcaatcc acatgcggtg gtcgatgcga
      721 cggttcgtga aggtggcgac acttggttct tcatcactgc ggactatgcg tatggccagc
      781 agctggcacg agactccgcc tcgttcgtgg tcgcaggcgg ggggaaggtg ctgggggaaa
      841 ttcggcatcc ctttcccggc acgaccgact tttcgtcctt tctactggcc gccaaggcaa
      901 gcggcgcgaa agtgatcgct ttcgcaaatg gaggagccga ttccgataac tgcatcaagc
      961 aagcggccga gttcggcctt gtagctggag gtatcaaggt tgtcggcgtt ggactgctcg
     1021 tcaattcgct gcccagcatt ggcctgacgg ccgcacaggg cgtgcggttc tccgagccgt
     1081 attattggga cctcaacgac gacacgcgtg ccttcggtcg gcgctaccaa gcgcagatca
     1141 agggtgcttc gatgccgaat tcggcccagg cgggtcagta ttcggccgta tcacactacc
     1201 tgaaagcggt ggtggcaatg ggaccggaga aggccaaagc cagcggacgg gcgaccgtcg
     1261 cccagatgaa gatgatccct atccaggatc agatcttcgg caaaagcgag atccgcgagg
     1321 acggccgcgt gatccacgcg atgtatctgt tcgaggcaaa ggcgccgaac gagtcgaagg
     1381 aacaatggga cgtggtgaag gtgaaaagca cgatcccgat ggaaaaggca tttcgaccaa
     1441 tcaatgaggg caagtgctca atgattcgaa cgtagctggt gtaacaggga cctctctggc
     1501 cgacgatcct tttacgtgcc c
//
LOCUS       DASWQN010000139         3663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_410999, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000139 DASWQN010000000
VERSION     DASWQN010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410999"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VGC82_02280"
     CDS             <1..784
                     /locus_tag="VGC82_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0422129.1"
                     /translation="SAQNARSVAIRIVSRVGEAVLGLGLLSARTKADVEATLRGAGHS
                     GTSALRLFVGAKIMLMLALPAGIWLLAAGMDVSFPIEALAIAAAVIGGMLAPDSVVRK
                     LRNGTMKRIQDELPDALDMMVICAQAGLGLGTTIVRVAQELRVTHKAVAVELAQTANE
                     LQMMTDAKLPLIGFGERCGVDSAKRLASTLVQSAQYGTPLTESLRGLAAELRTEMIMR
                     FEARAARMPVLMTLPMVAFILPSLFLVIGGPAVIQVIHGFNS"
     gene            768..1508
                     /locus_tag="VGC82_02285"
     CDS             768..1508
                     /locus_tag="VGC82_02285"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0422130.1"
                     /translation="MVSIRKIPLVVSLLLAIAGCSSPAPTESSKEATISDAALAAGTP
                     DIALHLADDALAHDPGDASALTRRGLALTALGRLQEARESLRKVVTSQPRNSRALLAL
                     GRVELPVDPASAEGRFQAILLQEPRNAAALNNLGIARDLQDRHGDAEPAYRAALAASP
                     DMTAAKVNLALCLAIRGQGSEAIALLRPMADAPTATRKMQQDFAAVLAMSGERVEAER
                     ILSTDMAMNDIAPALAELASARVGNVTR"
     gene            complement(1532..2863)
                     /locus_tag="VGC82_02290"
     CDS             complement(1532..2863)
                     /locus_tag="VGC82_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY0422131.1"
                     /translation="MTDYVSLVSELIRQARAAGADTADAVLVNGTSLSVASRQGKIEH
                     LERSEGRDLGLRVFVGKRAAIVSSTSLDPAGFAGLVERAVAMAKVVPEDPFAGLAETW
                     ATPDDTDLDMADPAEPAPDVLSARAAAAEEAAFSVKGVTNSEGAEAGYGRSEVVLVTS
                     AGFAGRRVGTSHSISATALAGTGTDMQRDYDYHTTVYFDDLDDPVKIGKSAGERAIAR
                     LNPTRPKTARIPVIYDPRVAGGLLGHLAGAINGAGVARGTSFLKEKLGQRIFAAGVNI
                     HDDPRRVRGLRSRTFDGEGTPTHARALIQDGVLTTWLLDSRSARQLGMTPTGHASRGT
                     SGPPSASTTNLYLAAGSMTPQALMADIKEGLYITEMIGMGVNGVTGDYSRGAAGYMIR
                     DGALAEPVAEVTVASNLLEMFANMVPASDLVFRRGTDAPTVRIDGMTMAGG"
     gene            3077..>3663
                     /locus_tag="VGC82_02295"
     CDS             3077..>3663
                     /locus_tag="VGC82_02295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY0422132.1"
                     /translation="MARLDGCYNVFDLREAAKRRLPKAVFEFVDRSTEDELALRNNRH
                     AFEKIKLKHRSLVDVSGRSTKTTLFGKEISLPMAIAPTGAAGLCWHEGELELAKAAAR
                     AKIPFTLATGAMTSMEKIAKEANFRLWFQLYVWKQRELSYQLIERAKNNGFEALIVTT
                     DTIVPPNREYNAKNGFLLPFHPTWRFTWDIMQHPT"
BASE COUNT          644 a         1156 c         1206 g          657 t
ORIGIN      
        1 atcggcgcaa aacgcccggt cggtcgcgat ccggatcgta tcccgtgtcg gtgaggcagt
       61 gctcggcctt ggcctgttgt cagcccgcac taaggccgac gtggaagcca cgctgcgggg
      121 ggctggtcat tccggaacct cggccttgcg tctgttcgtc ggcgccaaga tcatgctgat
      181 gctggccctg cccgccggca tctggctgct tgcggccggc atggatgttt cgttcccgat
      241 cgaggctctc gccatcgccg cggcggtgat cggtggcatg ttggcgcccg attcggtcgt
      301 acgaaagctg cgcaatggca caatgaaacg gattcaggat gagctgccgg acgcgctgga
      361 tatgatggtg atctgcgccc aggcgggcct gggcctcgga acgacgatcg tgcgcgtggc
      421 gcaggagttg cgcgtgactc acaaagcggt cgcggtggaa ctggcacaga cagcaaacga
      481 gctgcagatg atgaccgacg ccaagctgcc gctgatcggt tttggtgagc gttgcggcgt
      541 cgatagcgcc aaacggctgg cctcgacgtt ggtgcagtcg gcccaatatg gcacgccgct
      601 gacggagtcc ctgcgcggcc tggcagcgga gctgcgtacc gaaatgatca tgcggttcga
      661 ggcgcgagcc gcgcgcatgc cggtgctgat gaccttgccg atggtcgcat tcattcttcc
      721 cagccttttt ttggttatcg gcggacccgc cgtcattcag gtgatccatg gtttcaattc
      781 gtaaaatccc gcttgtcgtg tcgctgctgt tggcaatagc cggctgttcg tccccggcgc
      841 caaccgaatc atcgaaggag gcgacgatca gcgatgccgc gttggccgcc ggaacccccg
      901 acatcgcgct gcatctcgcc gacgacgcgc tggcgcacga tccaggcgat gccagcgcac
      961 tgacccgccg cggactggca ctcaccgcgc ttggccggct gcaggaggcg cgtgaaagct
     1021 tgcgcaaggt ggtaacgagc cagccgcgga attcacgagc gctgctcgca ctggggcggg
     1081 tggagcttcc ggtcgatccg gcgtcggcgg agggacgatt ccaggccatt ctgctgcagg
     1141 agccgcgtaa tgccgctgcg ctcaacaatc ttggcatcgc gcgggacctg caggatcgtc
     1201 atggcgacgc cgaaccagcc tatcgtgccg ccttggccgc gtcgccggat atgacagcgg
     1261 cgaaagtgaa tctggcactg tgcctggcga ttcgcggcca gggcagcgag gctatcgcct
     1321 tgctgcgccc catggcggac gccccgacgg cgacccgcaa gatgcagcag gactttgccg
     1381 ccgtcctggc tatgtcaggc gaacgcgtgg aggcggaacg tatcctgtcc accgacatgg
     1441 ccatgaacga tatcgcgccg gcactcgctg aactggcatc ggcgcgggtg gggaacgtaa
     1501 cgcgatgaca gggtgatcct cggtttgtgg ttcagccgcc cgccatcgtc attccgtcga
     1561 tccgaaccgt gggcgcgtcc gtgccgcgcc ggaacacaag atccgatgca ggcaccatat
     1621 tcgcaaacat ttccaatagg ttactcgcga ccgtgacctc ggcgaccggc tcggccagtg
     1681 caccatcacg gatcatgtag ccagcggcac cccggctgta gtcgccggtg acgccgttca
     1741 cgcccatgcc gatcatctcg gtgatataca ggccttcctt gatgtcggcc atcagcgcct
     1801 gaggcgtcat gcttccggcc gccagataca ggttggtggt cgaggccgac ggcgggccgc
     1861 tggtgccgcg cgatgcgtgc ccggttgggg tcatgccgag ttgccgagcc gaccggctat
     1921 ccagcagcca tgtcgtcagc acgccgtctt ggatcagcgc ccttgcatgg gtgggggttc
     1981 cctcaccgtc gaaggtcctg gaccgcagtc cgcgaacccg gcgcgggtcg tcgtggatgt
     2041 tgacgccggc ggcgaagatg cgttggccca gcttctcctt caggaaactg gtgcctcgtg
     2101 ccaccccggc gccattgatc gcaccggcga ggtgacccag caggccgcca gcgacgcggg
     2161 gatcatagat gaccgggatc ctggcggtct tcgggcgcgt cgggttcagg cgggcgatgg
     2221 cgcgttcgcc ggcgcttttg ccgatcttca ctggatcgtc cagatcatcg aaatagaccg
     2281 tggtgtgata gtcgtagtca cgctgcatgt cggttccggt ccccgccagc gcggtggcgg
     2341 agatcgaatg gctggtgccg acacgccgtc cggcgaaacc cgcggacgtc accaggacca
     2401 cctcgctacg gccatagccg gcttccgcgc cctcggaatt ggtaacgcct ttgacagaga
     2461 aggcggcctc ctcggccgcg gcggcacgag cggacaggac atccggcgcc ggttcggcgg
     2521 gatcagccat atccaggtcg gtgtcatccg gcgtggccca ggtctctgcc aagccggcga
     2581 acggatcctc gggtaccact ttcgccatcg ccacggcgcg ttcgaccagg ccggcgaaac
     2641 cggcgggatc gagcgacgtg gaggacacga tagccgcacg cttgccgacg aaaacccgca
     2701 ggcccaggtc ccggccctcc gatcgttcca gatgctcgat cttcccctga cgggacgcca
     2761 cggacagaga agtgccgttt accagcaccg catccgccgt atcggctccg gcggcgcgcg
     2821 cctggcggat caactcggat acgagtgaga cgtagtcggt catgcagtca gttcctccgc
     2881 ggcgggccgt gcggcgttca tagaagattt gcagccggag ccatagatag tgtggcgaca
     2941 aaggattgca gcacggaccg cggcaggaat gcctttcgcc aggctcctgg gggactcggg
     3001 gagtcccggt caccaaccaa atgccttgac accgccctgc cccgcttcat cattccgcca
     3061 tcaaggggga acggatatgg ccaggctcga cggttgctac aacgttttcg atctgcgcga
     3121 ggcggcgaag cggcgcttgc cgaaagccgt gttcgagttc gtggaccggt ccaccgaaga
     3181 tgaattggcg ctccgcaaca atcgccatgc gttcgaaaag atcaagctga agcaccgctc
     3241 gctggtggat gtttctggcc ggtccaccaa gaccacgctg ttcggcaagg agatcagcct
     3301 gccgatggcg atcgcgccga ccggagcggc cggcctgtgc tggcacgaag gggaactgga
     3361 actggccaag gcggccgctc gggcgaaaat ccccttcacc ttggcgaccg gcgcgatgac
     3421 atcgatggag aagatcgcca aggaggcgaa tttccgtctg tggttccagc tttacgtctg
     3481 gaaacagcgg gaattatcgt atcagctgat cgagcgtgcc aagaacaacg ggttcgaggc
     3541 gctgatcgtg accaccgaca cgatcgtgcc gccgaaccga gagtataacg cgaagaacgg
     3601 atttttgctg ccgttccatc cgacctggcg gttcacctgg gacatcatgc agcacccgac
     3661 ctg
//
LOCUS       DASWQN010000140         3447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2258544, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000140 DASWQN010000000
VERSION     DASWQN010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258544"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VGC82_02300"
     CDS             <1..730
                     /locus_tag="VGC82_02300"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0422133.1"
                     /translation="ADTAEIPEGNGHGGARSMHMGGAALFKAAQEVIEKGKPVAARLL
                     QAQPDQVVFAAGRFSAGDRSVDLLTVARDTSLDTYVWNLLDIITFPNGCHVAEVEIDP
                     ETGQVTLDRYTAVDDYGTLINPLLTIGQVQGGVAQGIGQALTEHTVYDPDSGQLLSGS
                     FMDYQIPRAIDLPDLDITLTGIPTTANPLGVKGSGQAGAIAAPQTIISAILNALAPLG
                     VTHIDMPATPERVWRVIQQAGNRA"
     gene            727..2406
                     /locus_tag="VGC82_02305"
     CDS             727..2406
                     /locus_tag="VGC82_02305"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEY0422134.1"
                     /translation="MTRIAAEDYLEALAAHGIEHLFVNPGTDFAPIVEAFSRAARSNR
                     KVPRPMVVPHENAAVGMAHGYTMVTGKPQAVMLHTNVGTANAINMLINASRDRVPMLL
                     TSGRTPFTEHGAEGSRSAHIQWAQEMFDQAGMLRELVKWDYEMKRGDQAAAVVDRALE
                     IAGASPQGPCYLSLPREVLGEVLPNPAESNRAHRAIPRPPEARSSDIALLADWIAAAK
                     NPLIITGMLGKDPRDSVVLSRLAERYALPVVPYNTRYFALSADHPMFQGSMPEPLLRD
                     ADLVIVFETDVPWLPSKMSPPPGARVVQIGEDPLHARLPMRGFPSDLTLTATCLSVLE
                     ALEAALPGRPAPHVADRDKRLRERSRKLHDTWRQEAESAGQAEIITLPWLNHCLRAVV
                     DADTKVISEYSFRQEYCPLHSPGSLFALSSAGGLGWGFPASLGAKLASPQSLVLSVLG
                     DGAYMFANPTACHYTAQMQKLPVLTIIYNNALYGAVRHATLDMYSKGIASEADGRLLA
                     NLPSVSFEKIVEAHDGYGEKVVLPSDLPAALQRAVAAVGSGRQALLNVVCA"
     gene            complement(2443..>3447)
                     /locus_tag="VGC82_02310"
     CDS             complement(2443..>3447)
                     /locus_tag="VGC82_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0422135.1"
                     /translation="MTLSLNAAVFVYHDGPEIAFEALCQQSLGRPDFLISGERWLFLR
                     PPFDLMPEGNRMPRCFCCGPTSFDRRGLLKTAFWATALSAAGLPALGQAPPASAGPAN
                     AALLEDLVAANRILYDQGVVDGFGHVSVRHDKDSARYLLSRSMAPALVTADDILEYDL
                     DSNPTDPKGRTSYLERFIHGEIYKARPDIVAVVHSHSPAVIPFADTTVPLRPMNHIGG
                     FLGSGAPVFEIRDAAGPASDMLIRNPSLGHALALSLGTHSVALMRGHGSVAVAQSIRH
                     VVFRAVYTEVNARMQSEAMKLGTPTFLNPEEAAAAAKTNDGLVDRPWALWKQRAMGKT
                     "
BASE COUNT          633 a         1123 c         1112 g          579 t
ORIGIN      
        1 ggccgatacc gccgagatcc ccgaaggcaa cggccacggc ggcgcccgtt cgatgcacat
       61 gggcggcgct gccttgttca aggcggcaca ggaagtcatc gaaaagggca agcctgtcgc
      121 cgccaggctg ctgcaagccc aaccggacca ggtggtattc gccgccggac gtttctccgc
      181 cggcgaccgc tccgtcgatc tgctgaccgt cgcgcgcgac acgtcgttgg acacctatgt
      241 ctggaacctg ctcgacatca tcaccttccc caacggctgc cacgtcgccg aggtcgaaat
      301 cgatccggaa accggccagg tgacgctgga tcgctacacc gcggtcgatg actacggcac
      361 gctgatcaac ccgttgctca ccatcggcca ggtccaggga ggcgtcgccc aaggcatcgg
      421 ccaggcattg acggagcata cggtttacga ccccgattcg ggccagttgc tgagcggatc
      481 gttcatggac tatcagattc cgcgcgccat cgacctgccc gacctcgaca tcacactgac
      541 cggcattccg accaccgcca atccgctcgg tgtgaagggt tcaggccagg ctggggcaat
      601 cgccgcgccg caaaccatca taagcgccat actgaacgcg ctcgcgccgc tcggcgttac
      661 ccatatcgac atgcccgcca cgccggaacg ggtctggcgc gtcatccagc aggcaggaaa
      721 tcgcgcatga cccggatcgc tgccgaagac tatctggaag ccctcgccgc ccatgggatc
      781 gaacatctgt tcgtcaatcc cggcaccgat ttcgccccga tcgtcgaggc gttttcccga
      841 gcggcacggt ccaatcgtaa ggtgccccgg cccatggtgg tgccgcatga aaatgccgcc
      901 gtgggcatgg cgcacggcta cacgatggtg accggcaagc cgcaggcggt gatgctgcac
      961 accaatgtcg gcacggccaa tgcgatcaac atgctgatca acgccagccg ggaccgggtg
     1021 ccgatgctgc tgacctccgg ccgcacgcca ttcaccgaac atggcgccga ggggtcgcgc
     1081 agcgcgcata tccagtgggc gcaggagatg ttcgaccagg ccggcatgct gcgtgagctg
     1141 gtcaagtggg actacgaaat gaagcgcggc gaccaggccg ctgcggttgt cgatcgcgcg
     1201 ctggagatcg ccggcgcctc gccgcaagga ccgtgctacc tcagtctgcc gagggaagtg
     1261 ttgggcgagg tcctacccaa cccggccgag agcaatcggg cacatcgggc gattccgcgc
     1321 ccaccggaag ctcgttcaag cgacatcgcc ctgctggcgg actggatcgc ggcggcaaag
     1381 aacccgctga tcatcacggg aatgctgggc aaggatccgc gcgacagcgt ggtgctgtcg
     1441 cggttggccg aacgctacgc gctgcccgtc gtgccctata acacgcgata cttcgcgctg
     1501 tcggcggacc acccgatgtt ccagggttcg atgcccgaac cgctgctgcg ggacgccgac
     1561 ctggtgatcg tgttcgagac cgacgtgccg tggcttccca gcaagatgtc gccgccgccg
     1621 ggtgcgagag tggtgcagat cggcgaagac ccgctgcacg cccgcctgcc gatgcgcggt
     1681 ttcccctccg acctgaccct gacggccacc tgtttgtccg tgctagaggc gctggaggct
     1741 gccttgccag gccgccctgc cccgcatgtc gccgaccgcg acaagcgcct gcgggagcgg
     1801 tcgcgcaagc tgcacgacac ctggcggcag gaggctgaaa gtgcgggcca ggcggagatc
     1861 atcaccctgc cctggctgaa ccattgcctg cgcgcagtgg tcgatgccga cacgaaggtg
     1921 atcagcgagt actcgttccg ccaggagtat tgcccgctgc attcccccgg cagcctgttc
     1981 gccttaagct ccgccggcgg gctgggatgg ggattcccgg cctccctggg tgccaagctg
     2041 gcctcgccgc aaagcctcgt gctttcggtg cttggcgatg gcgcctacat gttcgccaat
     2101 ccgaccgcat gtcactacac ggcgcagatg cagaagctgc cggtgctgac gatcatctac
     2161 aacaacgccc tgtacggcgc ggtgcggcat gcgacgttgg acatgtacag caagggcatc
     2221 gccagcgagg cggatggcag gctgttggcc aaccttccct cggtatcctt cgagaagatc
     2281 gtggaggcac acgatggcta cggcgagaaa gtcgtactcc ccagcgacct tcccgccgcc
     2341 ctgcaaaggg cagttgccgc cgtcggttcg ggtcggcaag ccctgttgaa cgtcgtctgc
     2401 gcctgatcgt cgagaccagc gaaaccggga gcggcggggt cgtcaggttt ttcccatcgc
     2461 gcgctgcttc cacagtgccc atggacgatc gacaagcccg tcgttggtct tcgccgccgc
     2521 agcggcctcc tcggggttca ggaacgtcgg agtccctagc ttcatcgcct cggactgcat
     2581 ccgggcattc acttccgtat agacggcgcg gaatacgacg tggcggatcg actgtgccac
     2641 cgcgaccgaa ccgtgtccgc gcatcaaggc aacggaatgg gtgccgagcg ataaagcgag
     2701 cgcatgaccc agggagggat tgcggatcag catgtcggag gcgggcccgg cggcgtcgcg
     2761 gatttcgaac accggggctc cgctgcccag gaagccgccg atatggttca tcggccgcaa
     2821 aggcactgtc gtatcggcga acggaatgac cgcgggggag tggctgtgca cgactgcgac
     2881 gatgtccggc cgcgccttgt agatctcgcc gtggataaag cgctcgaggt aggaggtcct
     2941 gccttttgga tccgttgggt tgctgtcgag gtcgtattcc aggatgtcgt cggcggtaac
     3001 cagggccggc gccatgctgc gggacagcag ataccttgcg gagtctttgt cgtgccgcac
     3061 gctgacatgg ccgaatccat cgacgacccc ttggtcatag aggatgcggt tggcagcgac
     3121 caaatcctcc agcagcgcgg cattggccgg cccggctgaa gccggtggtg cctgccccag
     3181 cgccggcagg ccggcagccg aaagagccgt ggcccagaac gcggtcttga gcaagccacg
     3241 acggtcgaac gacgtgggtc cgcagcagaa acatctgggc attcggtttc cttccggcat
     3301 gaggtcgaac ggcggacgca agaacagcca gcgttcaccc gaaatcagga agtccggccg
     3361 gccaaggctt tgttgacaga gcgcttcgaa cgcgatctca ggtccgtcat gataaacgaa
     3421 gactgcagcg ttcaaagata gtgtcat
//
LOCUS       DASWQN010000141         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_15896, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000141 DASWQN010000000
VERSION     DASWQN010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15896"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VGC82_02315"
     CDS             complement(<1..712)
                     /locus_tag="VGC82_02315"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0422136.1"
                     /translation="MSEPSRRLFLSLTAAALATGRAAIAQQPAPPSVITSAPRAWGRD
                     APPAMYPDPDVIVVDPAFLPLRVGNTAIHRVASGFQWAEGPAWCSEGHYLVFSDVVAG
                     VQYRFIWDDQRVSRFRAPSANSNGNAFDFQGRQISAQHYNRRVVRWEHDGTMTVIADT
                     FEGKHLNSPNDLVAHPDGSIWFTDPPYGGRLSEGHPDDAGGPTNPTGAFNPRIGAEDV
                     VPTSKRELPTNVYRWDPTG"
     gene            complement(859..1329)
                     /locus_tag="VGC82_02320"
     CDS             complement(859..1329)
                     /locus_tag="VGC82_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422137.1"
                     /translation="MKSLIYGLAALCLGGAVSPSRAGDVYVVDNAKGKRPMVTQKDGF
                     ARIPVPVTNGGQNQGYSSAMMFATPATGVTVYTGHMDPGGKIAHHEGGTVYVLYVVRG
                     TGKLVNVDPAGATTSEIAYKPDDVIVFQPNTHHLWENGPEPFDFVGVSQPQPKP"
     gene            complement(1862..2104)
                     /locus_tag="VGC82_02325"
     CDS             complement(1862..2104)
                     /locus_tag="VGC82_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422138.1"
                     /translation="MARIRSLACCTATVFVISRTAAFAALYAMWILSLPMMPAIKDRL
                     TTDPPPLAIIAGTACLMPRNPLVALMAMMRCQASVL"
     gene            2122..2304
                     /locus_tag="VGC82_02330"
     CDS             2122..2304
                     /locus_tag="VGC82_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0422139.1"
                     /translation="MGTSARTADPAVRAERTRVIPMRRPGEVDEVGYAILFLASNKSS
                     YVTGSEIHVDGGAIAI"
BASE COUNT          422 a          716 c          784 g          402 t
ORIGIN      
        1 taccggttgg atcccatcgg tacacgttgg tcggcagctc gcgttttgag gtgggcacga
       61 cgtcctcggc gccgatgcga gggttgaacg cccccgtcgg attggtcggg ccacctgcat
      121 cgtcgggatg tccctccgac aggcgcccac catagggcgg atcggtgaac cagatcgatc
      181 cgtcggggtg cgcgacaagg tcgttgggcg aattgaggtg cttgccctcg aacgtatcgg
      241 caatgacggt catggtgccg tcgtgttccc agcgcacgac ccggcggttg taatgctgcg
      301 ccgagatctg ccgcccctgg aagtcgaacg cgttgccatt gctgttcgcg gacggcgcgc
      361 ggaaccggct gacccgttgg tcgtcccaga tgaaacggta ttgcaccccc gcgaccacgt
      421 cgctgaagac caggtaatga ccttcactgc accaggctgg gccttccgcc cactggaagc
      481 cggaggcaac gcggtggatc gcagtgttgc cgacacgcag cggaagaaac gcaggatcga
      541 cgacaatcac atcgggatcg ggatacatcg ccggcggcgc gtcgcgtccc caggcccggg
      601 gcgcagaggt gatgacgctt ggcggcgcag gctgctgggc gatcgcggcg cggccggtgg
      661 ccagcgccgc ggcggtgagg cttaggaaca ggcggcggga cggttccgac atgggcttcc
      721 tccggttgtt attgcctccc cggcgcattc tctcgctcgg ggcggtggct ctggcgccgt
      781 acgaggtcgc ggttgaaggc gggcaggggc ggcaagacac ctcagtctga tgtcagcgta
      841 tatccgagac gagccctctc agggcttcgg ctgtggctgg ctgaccccga cgaagtcgaa
      901 cggctcaggc ccattttccc acaggtgatg ggtgttcggt tggaacacga tcacatcgtc
      961 gggcttgtag gcgatctcgg aggttgtggc acccgccggg tcaacgttga ccagtttgcc
     1021 ggtgccacgc accacataga gaacgtaaac cgtgccgccc tcatgatggg cgatcttgcc
     1081 gccaggatcc atgtggccgg tataaaccgt gactcctgtt gccggcgtgg cgaacatcat
     1141 cgccgagcta tagccctggt tttgaccgcc gttggtgaca gggaccggaa tgcgcgcgaa
     1201 accgtccttc tgcgtcacca tggggcgctt gcccttggcg ttgtcgacca cgtagacgtc
     1261 gccggcccga ctcggcgaaa cggcaccgcc caggcagagc gcggccaatc catagatcaa
     1321 ggacttcatt gtttcctccc atgtcgtgcg tcagcggcgc ccgcgccgat ggcgaacccg
     1381 atgagtatcg cgccgcgccg ccaatgagaa gtcatcggcc tcgccgggca gcggccacga
     1441 catggcgctt cttgtggcag cagcccaacc ggctgggcgg gctggcatca gtgcccattc
     1501 gggcgatgct tcctaacctc cggccggcgt ggcagcgtgt atccaagaga accagcgggg
     1561 ggagcaataa ccatgagacg caagggaaag atcgctttga tttccggcgc ggcatcggga
     1621 aagggcgcgg ccacgacgcg aatgtttgcc cgcgaaggcg ccaaagtcgt gatcgcggac
     1681 atgttggctc atgaaggccg gcaggtggcg gatgccatcg gcgctgccgc gcggttccag
     1741 gctttggacg tgaccaaaga agagaacggg gaagccgtcg tgggcgaaat cggcggcagt
     1801 tcggcaagcc ggatatcctg gtcaacaatg ccggcgtctc cggcagcgcc gaacaggatc
     1861 tttacagcac cgatgcctga catcgcatca tggccatcaa tgcgaccagc gggttccttg
     1921 gcatcaagca cgccgttccg gcaatgatcg ccaacggagg cgggtcggtc gtcaatctgt
     1981 ccttgatcgc cggcatcatc ggcagcgaga ggatccacat ggcgtacaat gccgcgaagg
     2041 cggcggtgcg gctgatgacg aaaacggtcg cggtgcagca cgccagggac cggattcgcg
     2101 ccaactcaat ccatgccggc gatgggcacg tccgcccgca ccgccgatcc cgcggtacgg
     2161 gccgaacgga cgcgcgtcat tcccatgcgc cggccgggag aggtcgatga ggtcggctac
     2221 gcgatcctgt tcctggcgtc gaacaaatcg tcatacgtca ccggctcgga aatccatgtg
     2281 gacggcggcg ccatcgcgat ctgaagcgaa agggtaattc cgcc
//
LOCUS       DASWQN010000142         3772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2390817, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000142 DASWQN010000000
VERSION     DASWQN010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2390817"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VGC82_02335"
     CDS             complement(<1..488)
                     /locus_tag="VGC82_02335"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY0422140.1"
                     /translation="MDDPRPMPVSNMPEYTVSEISGAVKRTLETTFGRVRVRGELTEV
                     KRYPSGHIYLSLKDENAKISGIVWRTSVPRLGMVPENGTEVIATGRISAFSERSSYQL
                     IIERMEYAGVGALLARIEMIRQRLAAEGLFDADRKRVLPGLPRVIGVVTSERGAVIQD
                     IK"
     gene            594..1862
                     /gene="purD"
                     /locus_tag="VGC82_02340"
     CDS             594..1862
                     /gene="purD"
                     /locus_tag="VGC82_02340"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY0422141.1"
                     /translation="MRVLVVGSGGREHALCWAVSGSPLLTKLYCAPGNPGIAAVAECV
                     NIGVLEIPTLVAFVQDNAIDLVVPGPEAPLVAGLADAMEAAGIACCGPSRAAARLEGS
                     KSFAKEIADAASIPTALWERFDDAGAAREFVRRRGAPIVVKANGLAAGKGVVVATTID
                     QAVAAIDAMMDQKIFGEAGVSVVIEECLTGKEVSLFALCDGEDALLLGSAQDYKRAGD
                     ADTGPNTGGMGAYSPVPGFPPKLEEACLEAIIRPALKEMARRGMKFRGILFAGLMLTD
                     DGAKLIEFNVRFGDPECQALLPRLKSDLLPALQAACDGELRDFDLRWNDQASVAVVMA
                     ARGYPDAPRHGSVIAGLEEAAAKPGAMVFHAGTQAGPDNTLLAAGGRVLTICGTAADV
                     PRARAVAYAAVDAIKWPEGFCRRDIGASTP"
     gene            1859..2626
                     /locus_tag="VGC82_02345"
     CDS             1859..2626
                     /locus_tag="VGC82_02345"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY0422142.1"
                     /translation="MSTTEAQRGDRIAKWLSRAGVASRRDAERMLAEGLVSLNGKTVK
                     HPATFIAAGDVVQVNGKVVGQPERTRVWRYHKPEGLVTTHKDPEGRPTVFEKLKHQLP
                     RVISVGRLDLTSEGLLLLTNDGGLARRLELPSTGWLRRYRVRVFGTPHEAALKSLAHG
                     VTVEGVRYGPIDAEIDARKGDNVWLTVSLREGRNREIRKVMEHLGLSVSRLIRVAYGP
                     FQLGVLERGGIEEIPARVLREQLPADAGAPPPLPRHR"
     gene            2628..3296
                     /locus_tag="VGC82_02350"
     CDS             2628..3296
                     /locus_tag="VGC82_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEY0422143.1"
                     /translation="MRIVAGAWRGRTLVAPPGQGTRPTADRVRQALFDMLLHAAWGGR
                     AVIEDAMVLDVFAGTGALGLEAMSRGAAFGCFIESDAAALQALRANVAACKAGDRVEI
                     LARDALDVGVLPRDRPASEPVGTDQAGRMWLAGPDAREVPKFSPSMGESKTRPASLVF
                     LDPPYGRNLVPRALTHLREAGRIAPNAVIVAEIGGGETWCPEQDLLAERRYGAARVLV
                     FRAT"
     gene            3303..>3772
                     /locus_tag="VGC82_02355"
     CDS             3303..>3772
                     /locus_tag="VGC82_02355"
                     /inference="COORDINATES: protein motif:HMM:NF038017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0422144.1"
                     /translation="MVNSVHLALALLWAHEPALLDTVALSLAVSVSASVAAAVVGLPL
                     GTALAIFEFRGRSILILIANALLGLPPVVVGLVLYLLLSRSGPFGGIGILFTPIAMTA
                     AQFLLALPIVVALSHRAMTVVWQEYGDQLLVDGIRRLQAIPHVLTIGRLGALTA"
BASE COUNT          599 a         1100 c         1381 g          692 t
ORIGIN      
        1 atcttgatgt cctggatcac cgcgccgcgt tcgctggtga cgacgccgat gacccgcggc
       61 aaaccgggca gcacccgctt gcggtcggcg tcgaacagcc cttcggcggc cagtcgctgg
      121 cggatcatct cgattcgcgc cagcagggct ccgacgccgg catattccat gcgctcgata
      181 atgagctgat aggatgaacg ctcgctgaag gccgagatcc ggccggtcgc gataacctcg
      241 gtgccgtttt ccgggaccat cccgagtcga ggaacggagg ttctccagac gatgccggaa
      301 atcttcgcgt tctcgtcctt cagcgacagg taaatgtggc cggacgggta gcgcttcacc
      361 tcggtcagct cgccgcgcac ccgcacccgg ccgaaagtgg tttccagcgt gcgtttcacc
      421 gcgcccgaga tctcggaaac ggtgtattcc ggcatatttg aaaccggcat ggggcgtgga
      481 tcgtccatgc ctgtagccta tgctagaccg tcccggagcg gtccagatct ttgttcgatc
      541 gcatgaccgg cccgcgttca tgctccaggg cgtggtgggc agaggagagt ccgatgaggg
      601 tgctggtggt cgggtcaggc gggcgggagc acgcgttgtg ctgggccgtt tccggcagcc
      661 cgctgttgac gaagctgtat tgcgcgccgg ggaacccagg aatcgcggcg gtggcggaat
      721 gcgtgaacat cggcgtgctg gagatcccga cgctggttgc gttcgtgcag gacaacgcga
      781 tcgacctggt ggttcccgga ccggaagcgc ctttggtcgc tggcctggct gatgcgatgg
      841 aggcggccgg catagcctgc tgcggcccct cacgagcggc agctcggctg gagggaagca
      901 agagcttcgc gaaggaaatc gccgatgcgg ccagcattcc cacagcgttg tgggagcggt
      961 tcgacgacgc cggcgcggcc cgcgaattcg tgcggcggcg gggggcgccg attgtggtga
     1021 aggccaatgg gctggcagcg ggcaagggcg tggtggtggc cacaactatc gatcaggcgg
     1081 tggccgcgat cgacgctatg atggaccaga agatatttgg cgaagcggga gtgtcggtgg
     1141 ttatcgagga gtgcctgaca gggaaggaag tttcactttt cgccctgtgc gatggcgagg
     1201 acgcactgct gttgggatcg gcgcaggatt acaaacgcgc cggggatgcc gataccggac
     1261 cgaatacggg tgggatgggg gcgtactcgc cggtgccggg ttttccgcca aaattggaag
     1321 aagcctgcct ggaagcgatc atccggccag cactgaagga aatggcgcgc cgggggatga
     1381 agtttcgcgg catcctgttc gccggcctga tgctgaccga cgacggagcg aaactgatcg
     1441 agttcaatgt ccggtttggc gatccggagt gtcaggcgct gctgcccagg ctgaaatccg
     1501 acctgttgcc ggcacttcag gcggcctgcg acggggagtt gcgcgatttc gacctgcgct
     1561 ggaatgacca ggcttccgtc gccgtggtga tggccgctcg ggggtacccg gacgcaccgc
     1621 ggcatggttc ggtcattgcc gggttggagg aggccgcggc aaagcctggc gcgatggtgt
     1681 ttcatgcggg cacccaggcg gggccggaca acacgcttct ggcggccggc ggacgcgttc
     1741 tgaccatttg cggcacggcg gcggatgtgc cgcgggcacg ggcggttgcc tacgcggcgg
     1801 tcgatgccat caagtggccc gaagggtttt gccgccgaga catcggcgcg agcacaccat
     1861 gagcactacc gaggcgcaac gcggcgaccg gatcgccaag tggctttctc gtgccggggt
     1921 cgccagccgg cgggacgcgg agcggatgct ggcggagggc ttggtcagcc tgaacgggaa
     1981 gaccgtcaag catccggcga ccttcatcgc cgcgggggac gtcgttcagg tcaatggcaa
     2041 ggtcgtgggg cagccggaac gtacccgggt gtggcgttat cacaagcctg aaggcctggt
     2101 gacgacgcac aaggaccccg aggggcggcc gacggtgttc gaaaagctca agcaccaact
     2161 gccgcgcgtg atcagcgttg gacggctcga cctgaccagc gaggggctgt tgctgctgac
     2221 caatgacggc ggcctggccc gccggctgga gctgccgtcc accggctggc tgcgccgcta
     2281 ccgggtgcgc gtgttcggca cgccgcacga ggccgcgctg aaatcgctgg cccatggcgt
     2341 gacggtggaa ggtgtgcgct acgggccgat cgacgccgaa atcgacgcca ggaaaggcga
     2401 caatgtttgg ctgaccgtca gtctgcgtga agggcgaaac cgcgagattc gcaaggtcat
     2461 ggaacacctc gggcttagcg tgtcccggct gatccgtgtc gcctatgggc cattccagct
     2521 cggcgttctc gagcgcggcg gcatcgagga gattccggca agggtgctgc gcgagcaatt
     2581 gccggcggat gcgggggcgc ctccgccatt gccgcgccac cgctaggatg cggattgtcg
     2641 ccggtgcctg gcgcggccga acgttggttg cgccgccggg gcaggggacg cggcccacgg
     2701 cggatcgagt acgccaggca ttgttcgaca tgctgttgca cgccgcgtgg ggcgggcgtg
     2761 ccgtgatcga ggacgcaatg gtgctcgatg tctttgccgg gaccggggca ttgggactgg
     2821 aggcgatgtc gcgcggcgcg gccttcgggt gtttcatcga gtcggatgcg gcggcgttgc
     2881 aggccttgcg ggcgaatgtc gcagcatgca aggcggggga tcgggtggag attttggcgc
     2941 gggatgcctt ggacgtcggg gttttgccgc gtgatcgccc ggcgagcgaa ccagtgggga
     3001 cggatcaagc tggccgcatg tggcttgcgg gaccagatgc ccgcgaggtc ccaaagttca
     3061 gcccgagcat gggggaatcg aaaacgcggc cggcatcgct ggtttttctc gacccgcctt
     3121 atggccggaa tctcgtgccg cgtgcgctga cgcatctgcg tgaagccggc cgtattgcgc
     3181 cgaatgccgt gattgtcgcc gaaatcggtg ggggcgagac gtggtgcccg gaacaggacc
     3241 tgctggcgga acgccggtat ggagcggccc gcgtcctggt attccgtgcc acttgatggg
     3301 acgtggttaa ctccgtccat ctggcgctcg cattgctgtg ggcgcacgaa ccagcgctgc
     3361 tggacaccgt cgcgctttcg ttggcggtga gcgtgtccgc atccgtcgcg gctgccgtgg
     3421 tcggactacc ccttgggacc gcattggcga tattcgagtt tcgcggccgg tccatactga
     3481 tcctgatcgc aaacgcgctg cttgggctgc cgccggtcgt cgtcggtctg gtgctctatc
     3541 ttctgttgtc acgctccgga ccgtttggcg gcattggaat tctgttcacg ccgatcgcca
     3601 tgacggccgc gcagttcctg ctggctttgc cgatcgtggt ggcgctcagc catcgcgcga
     3661 tgaccgtggt ctggcaggaa tatggggacc aactgctggt tgacggcata cggcggctcc
     3721 aggcgattcc gcatgtcctg actatcggcc ggctcggcgc ccttacggcc ag
//
LOCUS       DASWQN010000143         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_411561, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000143 DASWQN010000000
VERSION     DASWQN010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411561"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..681
                     /locus_tag="VGC82_02360"
     CDS             13..681
                     /locus_tag="VGC82_02360"
                     /inference="COORDINATES: protein motif:HMM:NF006623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0422145.1"
                     /translation="MDEQHGPRAGLFRLFTQIWNSVSLNDDADVTALPLPSERRLSNI
                     TPLPRQAFLLLSLEGFSEEEVAYVLGTDVAETRKLADSAGREMAAEIATDVLIIEDET
                     FIAMDLESLVKNLGHNVIGVARTHADAVALAKNKKPGLILADIQLADGSSGLDAVNEL
                     LRTFEVPVVFITAYPERFLTGERPEPAFLISKPFQPAMVSAVASQALFFQRNSRNRVP
                     RPAA"
     gene            1059..1433
                     /locus_tag="VGC82_02365"
     CDS             1059..1433
                     /locus_tag="VGC82_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422146.1"
                     /translation="MTLTLGAVPLALGRDLTGASGDVQAATAPAGIISTGVNRTAKTN
                     RVDAAARSATPTRTISLQVNGLAETSVLVRIPVADAAARRAPAPMLIKSGERKSTTVA
                     CEPMVSVLTEVAKQLQPGRCVT"
     gene            complement(1462..>1661)
                     /locus_tag="VGC82_02370"
     CDS             complement(1462..>1661)
                     /locus_tag="VGC82_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422147.1"
                     /translation="ACCGLGFWTGPLLRSASAGSQFNALAVAVALAFPDPAAEIFGLR
                     AHRDHWTRIRRRGGHFAEQRP"
BASE COUNT          300 a          563 c          513 g          285 t
ORIGIN      
        1 gacccgtcgc tgctggatga gcagcatggg ccgagggcgg gacttttcag gcttttcacc
       61 cagatctgga attcagtgtc gctcaatgat gacgcggatg tgacggcgct gccgctgccc
      121 tccgagcgcc ggctttccaa catcaccccg ttgccgcgtc aggccttttt gctgctgtca
      181 ctggaaggct tctccgagga ggaggtggcc tacgtcctgg ggaccgacgt cgccgaaacc
      241 cgcaagctgg cggattccgc cggacgggaa atggcggcgg aaatcgccac cgacgtcctg
      301 atcatcgagg acgagacctt cattgcgatg gatctcgaga gcctggtgaa gaatctcggc
      361 cacaacgtca tcggcgtagc ccgcacccat gccgacgcag tggcgctggc caagaacaag
      421 aagccgggcc tgatccttgc cgacatccag ctggcagacg gcagttcggg tctggacgcc
      481 gtcaatgaat tgctgcggac cttcgaggtg cccgtggtct tcatcaccgc ctatccggag
      541 cgtttcctga ccggcgagcg cccggagccg gcgttcctga tctccaagcc gttccagccc
      601 gccatggtct ccgcggtggc gagccaggcg ctgttcttcc agcgcaactc ccgcaacagg
      661 gtgccccgcc cggcggcctg aacggatcgc tcgaaatgaa tcaggccggc gcgccgcaag
      721 gcacgccggc ctcgcctttg gcgaaccgta aatgatcaaa cgaatgtcta tcggggaata
      781 aaagcagggc gcggctggca tcaccacagc cgcgccctac gtcgccggtc aatggggcag
      841 gggggataac cggctgccgt gaagacgcac agcccccctg actcgttcca gccccgacca
      901 gatttttttc cagccccgac cagatttttc aaacacggtt aagtgataac gcatttggcg
      961 aacccgctcg gtccggtcgc gttgtggtgg tgatcgccat ggggcgaggg gccaggcaca
     1021 tgtcacagtt ttcccgggga attttcgcag caatcgccgt gacgctgacg ctgggcgccg
     1081 tcccgctggc gttgggccgt gacctcacgg gcgcatcggg tgatgtccag gcggctaccg
     1141 ctcccgccgg catcatttca acgggtgtca accgtaccgc caagaccaac cgcgtcgacg
     1201 ccgccgcacg ttccgccacg cccacgcgga ccatttcgct ccaggtgaac ggccttgcgg
     1261 agacatcagt gctggtgcgg atcccggtgg ctgacgcggc cgctcgccgc gctcccgctc
     1321 ccatgctcat caaatccggc gagcgcaagt caacgacggt cgcctgcgag cccatggtga
     1381 gcgtcttgac tgaagtcgcc aagcagctcc agcccggccg ctgcgttacc tagcctcctg
     1441 cgaactcaag gccctgcgaa cttaaggccg ttgttcggca aaatgcccgc cgcggcgccg
     1501 tatccgcgtc cagtggtcgc gatgagcgcg cagcccaaag atctccgccg ccgggtcggg
     1561 aaacgccaac gccaccgcca ccgcgagcgc attgaattgc gagcccgcgc tggctgacct
     1621 caagagcggt cctgtccaaa agccgaggcc gcagcaagcc g
//
LOCUS       DASWQN010000144         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2127271, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000144 DASWQN010000000
VERSION     DASWQN010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2127271"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..2410
                     /locus_tag="VGC82_02375"
                     /pseudo
     CDS             1..2410
                     /locus_tag="VGC82_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438956.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
     gene            2448..>2692
                     /locus_tag="VGC82_02380"
     CDS             2448..>2692
                     /locus_tag="VGC82_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY0422148.1"
                     /translation="MATDTLQRPNVAAAKEENQVLVTLTVGDQLCGVPVLAVRDILGE
                     QVITRIPLAPPEIAGSLNLRGRIVTAIDLRRRLRLPPA"
BASE COUNT          503 a          909 c          843 g          437 t
ORIGIN      
        1 atcctgggga agctgcgcga ccgcagcctg accgtcaccc cggatatcgt cacacaggtg
       61 ctgagcgcga tcgaccagat caaggcaatc gtgacgtgcc tgggcgcaac cggcgccgaa
      121 cccgccggtg acgacgcgga attgatcgcc gcactgaatc tcaccgcggc gaacggcatt
      181 cgctcggccg tcgaccaacc gctggcgctc gaattcgatc agctcgccac tccggctgaa
      241 gcggttgtcg aagccgtcgc ggcggagcgg gcggaaaccg ccgtcccggc agcccgcgcc
      301 gaaccttcca cggctacgcc cgccgcggcg ccggccaccg caccttccgc ttcgcagacg
      361 atccgtgtga ccgtcgatgt gctggaagat ctgatgaccc tggtcagcga actggtgctg
      421 acccgcaacc agcttctgca attggcgcgc aaccaggaca acggcgcctt cacggttccg
      481 ctgcagcggc tgtcgcatat cacctcggat ctgcaggaag gcgtcatgaa gacccgcatg
      541 cagcccatcg gcaacgcctg gaacaagctg ccgcgactgg tgcgcgatct ttccaacgac
      601 ttgaacaaga agatcgagct caccatgctc ggtgccgaaa cggagctgga ccgtcaggtg
      661 ctcgagctga tcaaggaccc actgacgcac atgatccgca acagcggcga tcatgggctg
      721 gaaactccgg cggaacggcg ggccgccggc aagccggagg ccggcaacat cgtgctgaac
      781 gcctaccacg aaggcggcta catcatcctt gagatatccg atgacggccg aggcctggca
      841 gtggaccgca tccgcgcgaa ggcactctcc gccggcctgg ccaccgaggc ggagctgtcg
      901 gcgatgaccg acggacaaat ccagcgcttt atcttccgcg ccggtttttc caccgcggca
      961 acggtttccg ccgtatccgg ccgcggtgtc ggcatggacg tggtgaagac caacatcgaa
     1021 aagatcggcg gcacgatcga cctgaaaagc atccaaggcc acgggacgac gtttaccgtc
     1081 aagattccgc tcacgctggc gatcgtctcg gcattgatcg tcgaagccgg aaaagaacgc
     1141 ttcgcgatcc cacagctcag cgttgtcgaa ctggtccggg cgcagcgcag cttctccacc
     1201 ggtggccgcg aaagcgtcgg cagcagcgtg atcgaacgga tcaacgacac gccggtcctg
     1261 cgcctgcgcg accgcctgct gccgctggtc agcctgaccg aactgctgca tctcggatcg
     1321 gagcacgcgg ccgaaggccc cgcccatgtc gtcgtggcgc aagtcggccg gcacatcatg
     1381 ggcatcatcg tgggacgggg tgttcgacac cgaggaaatc gtcgtcaaac cggtcgcccc
     1441 aatccttcgc cacgtcacga tgttcagcgg caacacgatc ctgggcgacg ggtcggtcat
     1501 catgatcctg gaccccaacg gcatcgcccg agccagcagc gtcgtcgccg ggtccgagag
     1561 ccgcctgacg cgctctgcct tggtcgatac ccaaagctcc gccgacaaga cggcgatgct
     1621 gttgttccgg accggcgaga acacgcgcat ggcggttccg ctcgggctcg ttgcccgcct
     1681 ggaagacatc ccgcgcgaca agatcgaatc gtcctgcggc tctccggtca cacaatatcg
     1741 cggcaagctg atgccgttga tctcgatcga cggcagcgtc gatcagcaga agccgacaca
     1801 gccattgctg gtcttcgccg agggcgaccg catcatgggc ctgatggtcg atgagatcat
     1861 cgacgtggtg gaagacagcc tgcatatcga actcgccggc gctcgtccgg gcctgcttgg
     1921 atcggctgtc atcggcggcc tggccaccga cgtgctggat accggctact ggctgatgca
     1981 ggcgtgggaa gactggttcc gcggtgttcc gcgccaaggt gcctcggccg gccagatgca
     2041 tatcctgctg gtggacgaca gcgagttctt ccgtcagttg acggttccaa tgctcggggc
     2101 atccggcttc cgggtgaccg ccgtcgccag cgcgaccgag gcgctgcgcc tgcgcgatgc
     2161 cggcaacaag ttcgacgcca ttgtctccga catcgagatg ccgggaatgg acggcatcga
     2221 attcgcccgg gcggttcgcg ccgaaggtcc gtggaagcgg ctgccgttga tcgcgatgac
     2281 cgcccattcc gacatggaga acgctcgcat cgggcgcgaa gccggattca ccgattacgt
     2341 cgccaaattc gagcgtgaag cgctggtcgc cagccttcgt gccagcctcg cggaacccgc
     2401 ttacgcctga tcgcccgctc gcgcccgatc aaccaaagga accagccatg gccaccgata
     2461 cattgcagcg gccgaacgtt gcggccgcaa aggaagaaaa ccaagtcctg gtcaccttga
     2521 ccgtgggcga ccagctttgc ggcgttcccg tgctcgcggt gcgcgacatc ctgggtgagc
     2581 aggtcatcac ccgcattcca ctggcgccgc cggagatcgc cggcagcctg aacctgcggg
     2641 gccgcatcgt taccgcgatc gacctgcgcc ggcggctgcg cctgccgcct gc
//
LOCUS       DASWQN010000145         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1863555, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000145 DASWQN010000000
VERSION     DASWQN010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863555"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VGC82_02385"
     CDS             <1..675
                     /locus_tag="VGC82_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEY0422149.1"
                     /translation="AALAGLLHDGMTIMSGGFGLCGIPAALIEAIRDSGVKDLTIISN
                     NAGIDDVGLGLLLQTRQVRKMISSYVGENATFARQYLAGELEIEFNPQGTLAERIRAG
                     GAGIPAFFTATGAGTLIAEGKPQQEFDGRLYVQERGLRADLAVIHAWKADPEGNLIYR
                     KTARNFNPIMATAGETVVVEVEEIVGPGQIDGDHIVTAGIFVNRIVKLDTISKRIEQR
                     TVRKRA"
     gene            713..1369
                     /locus_tag="VGC82_02390"
     CDS             713..1369
                     /locus_tag="VGC82_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEY0422150.1"
                     /translation="MPWTRDQMAARAAKELEDGFYVNLGIGIPTLVANHIPEGMSIQL
                     HSENGMLGMGAFPFEGEEDADLINAGKQTVTELPTTSFFDSAASFAMVRGGHIDISIL
                     GALQVAENGDLANWMIPGKMVKGMGGAMDLVAGVRRVVVLMEHTAGGKAKLLKRCNLP
                     LTGAGVVDLIITDLGVFKVAAKGKDNGLMLLEMAPGVTLAELHEKTEAPFTLAEGVAA
                     "
     gene            complement(1392..2384)
                     /locus_tag="VGC82_02395"
     CDS             complement(1392..2384)
                     /locus_tag="VGC82_02395"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0422151.1"
                     /translation="MSRKPSQLRLKPGISWWRCNHNRAGWAAWEGKAVEAGFLGVGNM
                     GLPMAGKLMDGGHSLTIHDISEAAMLPLLQRQARRAASPKDLSDQCEIVFVSLPTLAA
                     FRSVAFGADGLARGKAMKLLVNTCTVGVPFVKEIEQAMAAQGVTVVDCPISGGPPGAR
                     AGTLSVMVSGDPAAVDRVRPMISLWGHTLTVAGNKPGAAQVLKLTNNILSAVALAATA
                     EAFVMGAKGGLDPEVILSAINAGSGRNSATEAKFPAAVLTRSFDYGAEMHILMKDIDL
                     AIAQGEDLGVPMWVCQAARLVFKHAMYQGAAHQDLTAIVKYVEQDAGFEIPKTR"
     gene            complement(2326..>2523)
                     /locus_tag="VGC82_02400"
     CDS             complement(2326..>2523)
                     /locus_tag="VGC82_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0422152.1"
                     /translation="GEILRCPWHGWEFDVRTGQSFCDPSKISVRSYPVEVADGQQVGQ
                     GPYVAETIPVTVEARYIVVEM"
BASE COUNT          474 a          799 c          816 g          434 t
ORIGIN      
        1 gcggcgctgg cgggactgct gcacgacggc atgacgatca tgtccggcgg gttcggcctg
       61 tgcggcatcc ccgcggcgct gatcgaggcg atccgagaca gcggagtgaa ggatctgacc
      121 atcatctcca acaacgccgg cattgacgat gtgggactgg gcctgctgct gcaaacgcgg
      181 caggtgcgca agatgatcag ctcctatgtc ggcgagaacg cgacattcgc ccggcaatac
      241 ctggccgggg agctggagat cgaattcaac ccgcaaggca cgttggcgga gcggatccgt
      301 gcgggaggcg cgggcatccc ggcgttcttc acggcgaccg gggcagggac cttgatcgcc
      361 gaaggcaagc cgcagcagga attcgatgga cggctgtatg tgcaggagcg cgggttgcgc
      421 gccgacctgg ccgtcatcca cgcctggaag gccgaccccg aaggcaacct gatctatcgg
      481 aagaccgccc ggaatttcaa tccgatcatg gccaccgccg gagaaacggt ggtggtggaa
      541 gtcgaggaga tcgtcggacc cgggcagatc gacggcgacc acatcgtcac cgccggcatc
      601 ttcgtcaacc gcatcgtcaa gctggatacg ataagcaaac ggatcgaaca acgcaccgtg
      661 cgcaagcgag cctgaaccgg accgattgca cgttttggag gagccgacag atatgccctg
      721 gacccgtgac cagatggccg ctcgggcggc aaaggaacta gaggacggct tctacgtgaa
      781 ccttgggatc ggcattccga ccctggtggc caaccacata cccgaaggca tgtcgatcca
      841 gttgcactcg gaaaacggca tgctcggtat gggcgccttc ccgttcgaag gcgaggaaga
      901 cgccgacctg atcaacgcag gcaagcagac cgtaacggag ttgccgacca ccagcttctt
      961 cgacagtgcc gccagcttcg cgatggtgcg cggcggacac atcgatatct ccatcctcgg
     1021 cgcattgcag gtggccgaga acggcgacct cgcgaactgg atgatccccg gcaagatggt
     1081 gaaggggatg ggcggagcga tggacctggt tgccggcgtg cgccgcgtgg tggtgctgat
     1141 ggaacatacg gcgggcggca aggcgaagct gttgaagcga tgcaatctgc cgctgaccgg
     1201 ggccggcgtg gtggacctga tcatcaccga cctcggggtt ttcaaggtgg cggcgaaggg
     1261 caaggataac gggctgatgt tgctcgagat ggcgcctggc gtgacattgg ccgagctgca
     1321 cgaaaaaacc gaggcgccgt tcactttggc cgaaggtgtg gctgcctgaa tcagcccgtc
     1381 ggccccctcg gtcagcgggt cttcggaatt tcgaagcccg cgtcctgctc gacgtatttg
     1441 acgatcgcgg tcaggtcttg gtgcgctgcc ccctggtaca tcgcgtgctt gaaaaccaac
     1501 cgagcggctt ggcagaccca catggggaca ccgagatcct cgccctgagc gatggcaagg
     1561 tcgatgtcct tcatcaggat gtgcatctcg gcgccgtagt cgaagctgcg tgtcagcacc
     1621 gccgcgggga actttgcctc cgtcgcgctg ttgcgcccgc ttccggcgtt gatcgcgctc
     1681 agtatcacct cggggtccag accgcccttg gctcccatga cgaaggcctc ggcggtggcg
     1741 gccaaggcga cggccgacag gatgttgttg gtcaatttca gcacctgcgc cgcgcctggc
     1801 ttgttcccgg cgacggtcaa cgtgtggccc cacagcgaga tcatcggccg cacccggtcc
     1861 accgcggccg gatcgcccga caccatgacg gacagcgtcc cggcccgcgc acccggcggt
     1921 ccaccggata tcgggcaatc cacgaccgtg acaccttgcg ctgccatggc ctgctcgatc
     1981 tccttcacga acggcacgcc gacggtgcag gtgttcacca gcagtttcat cgctttgccc
     2041 cgggccaggc cgtcggctcc gaacgcgacc gaacgaaacg ccgccagcgt gggcagcgag
     2101 acgaacacga tctcacactg gtcagagaga tccttcggcg atgcggcccg ccgggcttgc
     2161 cgttgcagca gcgggagcat ggctgcttcg ctgatgtcgt ggatggtcag gctgtgcccg
     2221 ccgtccatca gcttgccggc catgggtaat cccatgtttc cgacccccaa gaatcctgcc
     2281 tccactgctt ttccctccca agcggcccaa ccggcccggt tatggttaca tctccaccac
     2341 gatataccgg gcttcaaccg taactgggat ggtttccgcg acatacggtc cctgtccgac
     2401 ctgctgcccg tcggccactt ccacagggta gctgcggaca ctgatcttcg aaggatcgca
     2461 gaagctctgg ccggtgcgga catcgaactc ccagccgtgc caggggcagc gcaggatttc
     2521 gcc
//
LOCUS       DASWQN010000146         9369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_675568, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000146 DASWQN010000000
VERSION     DASWQN010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9369
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675568"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VGC82_02405"
     CDS             <1..469
                     /locus_tag="VGC82_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422153.1"
                     /translation="EQGDFLSLAGDAVSPNLLWGRLFAGWAPCAIAIAAGTAFKIADR
                     IGSRAAAVYQDQFDRDGAVAHAWLTRRGIELGGPVLRQIADLFDASPLQSDWRSSAIP
                     EQRLAGLVADPSMPAAHRRDAAEALLRFRARTAEMPPFPRLVTRPLGMLMLVP"
     gene            466..4119
                     /locus_tag="VGC82_02410"
     CDS             466..4119
                     /locus_tag="VGC82_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422154.1"
                     /translation="MMLDLEDCILAGSAITAPFLRDGLARVLDLGRDAADGWESVRLS
                     LRIGGAERVHRHVTMKLASAFGYGRPVRQSDVTTREGPEDGGWLLRASGGKQLRAWSI
                     PSDVDLDGLEQSPRSYRSSPTRIAQRVLLARGERAGLLTNGEVLRLLLSHPSRADSHL
                     SIGIGLWRQAPLPPDSFRVLKALAGVDGLARLPAVLEAARLHQARVTTELRRQAKEAI
                     VGFINALPSRTGLDPAALWHEGLVLVYRLLFILKLESPAEAGGGFSFASTRLWREALS
                     PNRALGPLVRRRLDHGHDTGRMLEAGLRSLFVAFRDGLRCSELHIAPLGGALFGSGTT
                     PLLDRLDWGDQAVAILLDRLIWLVSPRGERVRVHYGSLDVEDLGSIYEGLLEQQPGIA
                     TEPLIRVRHGKTEAVVPAARQQADIETGAFFLRSGMGRKASGSFYTPHDFVRFLVRET
                     LDSKIATLSPPDDPQPARLLTLKIVDPSTGSGHFLVEACRYLGEALLTACRLCDKKGL
                     HDRIAALPDPDQTIAAYLPSRGFSEARARAICRRLVAVHCLYGVDRNKLAVELAKLSL
                     WLESYAEGLPLTFLDHRLVHGDALSGPFFAALATLPVTGGALDPLLARGVADRLDCSL
                     RQARMLVAELNASIGRDLTDLGTKQVAKDRLDRLLHPLRQLARAWAGAAMLRERDSDD
                     VWLGLATHVAAKGCWPGTLSGGQAALLAAGSDAVAWDLVFPEVFPDGFSVVLGNPPWD
                     VLLPNTREFVADYDPTILDARTRSERAAIEHAALAQPAVAAAFSAYRDGFERMKNVAR
                     RLYRHQRVQAGGETTSGNMDLFRLFAERNMDLAAPTGAIGVLMPSAFHANEGTTGIRR
                     LYLQEADLSWCLSFENRRRIFDIDSRFKFDLIVAHRPGPTRSLRCGFYLDRIEDINDP
                     RKIMCYSMAFLYRTGGAGLAPLELRGNVDQRIADILFAQPERLGAWCADRHIRFGCDL
                     HMTADAGCFQPAGQGNTVLYEGKTFHQYSDTWDSKPRYSVASEALKPAIAEAARHYRL
                     AFRDIARSNDERTMIACIVPPGVVFGHTATVEKHPGARSVADALVLCALFNSFPFDWL
                     VRQKTATHLSLYILQALPVPRFGEAENSFLAQAALRLSCNNDGYANLWRDQVGGRQTA
                     LQQCWELRAAIDAVVAEAYGLDRDQYEHLLRSFSHRSDPGAPSLCLAAFDRLQAMGLA
                     DFCPHGGTEASAG"
     gene            complement(4108..5040)
                     /locus_tag="VGC82_02415"
     CDS             complement(4108..5040)
                     /locus_tag="VGC82_02415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0422155.1"
                     /translation="MKRLLLADRVKGRTDSFLAPLIPALEQHYEVRFIAAGPGDGLAE
                     AIGWADLVWLEWCWDHAVWATRQALPPGKLCVVRLHSIEALQTDYPARMDWTRVSRLI
                     TVADDIKDVLLTAHPGIADAVRIEVIPNGIDTARFRPGSPDRFRVGWVGHLEPKKNPM
                     LLMQIAHRLRQRDARFSFHVAGAFTDLRTARYLRQMQSALGLAGAVHFNGHVADMPAW
                     YADKGVLLSTSMYESFGMNIGEAMAAGAFPVVHGFPGADRLWPHECLFASIDEAVALT
                     GAARTGLYTDWVRDRYGLDRQIASVLDLLRGLSG"
     gene            5269..6219
                     /gene="lpxC"
                     /locus_tag="VGC82_02420"
     CDS             5269..6219
                     /gene="lpxC"
                     /locus_tag="VGC82_02420"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY0422156.1"
                     /translation="MDGLTQLVVDESGASSEWRQRTLKTAISCVGVGVHSGRRVNLTI
                     QPAAADHGIVFRRTDLDRVIEARFDNVCDTRLSTVLSDPAMASARVGTVEHLMAALSA
                     LGIDNALIDVDGPEIPILDGSAAPFVFLLDCAGWVEQQAPRRVIEIRRTVRVSAGNAW
                     AELRPLGPAVRAMRPVLELELSIDFAASAIGRQSASLRLTPDSFRHEIASARTFAQAA
                     EVEQLQAAGLARGGSLDNAIVVDGADILNAGGLRMANEFASHKLLDAVGDLALAGGKL
                     HGRFVASRTGHELNNRLLRSLFADETAWASVVTEPLVAAA"
     gene            6324..7193
                     /locus_tag="VGC82_02425"
     CDS             6324..7193
                     /locus_tag="VGC82_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007283526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY0422157.1"
                     /translation="MNTSRPTSFRSARGLFLGATTLLPLLSGCGSATDDSAKKGPSGP
                     VEELYNNGVDALSARRFSTAGDQFNAVEQNYPYSTWAVNAQLMSGYSLYLQNKYTEAI
                     STLDRFIQLHPAHRDVAYAYYLRALCYYEQIADIQRDQKGTEQAMNALREVVTRFPDT
                     TYARDAKLKIDLCFDHLAGKEMEIGRFYQKQHLYEAAIGRFQRVVDDFQTTNHVPEAL
                     ARLTEVYLALGLKDQARKTSAVLGYNYPGSEWYDASYGQLVDSGIASPADRSPSQEPE
                     RRGFLSRTWHAVF"
     gene            7227..8942
                     /gene="recN"
                     /locus_tag="VGC82_02430"
     CDS             7227..8942
                     /gene="recN"
                     /locus_tag="VGC82_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY0422158.1"
                     /translation="MLTALSIRDVVLIERLDLAFDHGLTVLTGETGAGKSILLDSLGL
                     ALGARAESGLVRASAEQASVTACFSSPNHPSIPSMLDEHGLTADAEIVLRRVVTKDGR
                     SRAFINDHPVGVGLLRRVGALLVEVQGQHEQMSLADPASHAGLLDAFGVPAELRIGVS
                     ETWHAWRNALRRLEQAREAIAAAERDEEWLRHAVDELATLAPRIGEEETLARERQRLQ
                     QSERRAEAIAAALAELTPRDRRNAGPAASLRSASRALQRLVPSGQPEAGGNPADTALA
                     ALERAEEALAEAETVLSRLIADADVDPRLLEQSEERLFALRAIARKHAVAVPDLPELL
                     DTLAARLTALETGASEIAALEQATRDTKDRYMAAAAALSAAREAAGGKLQKAVGKELP
                     PLRLDKARFVVEVGALPEAGWGPGGMDQVRFLIATNPGQEPGALARVASGGELSRLML
                     AMKVVLSAGSAVPTLVFDEVDSGIGGATAAAVGERLVRVAEGVQVLVVTHSPQVAARG
                     AAHLRVAKAATRDRTATTVDHLGPDARREEIARMLAGEIISDAARAAADDLLRGQRPQ
                     QGLLV"
     gene            9029..>9369
                     /locus_tag="VGC82_02435"
     CDS             9029..>9369
                     /locus_tag="VGC82_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY0422159.1"
                     /translation="MLEATARQAVETLTEAEAAEELEHLAREIAHHDAAYHTHDAPEI
                     SDADYDALRRRNSAIEARFPELIRADSPSSRVGGAPEPGFAKLRHRVPMLSLDNAFDA
                     AEFAEFCNRAKR"
BASE COUNT         1551 a         2970 c         3159 g         1689 t
ORIGIN      
        1 ggagcagggc gatttccttt cgctcgccgg tgatgccgtc agccccaact tgttgtgggg
       61 ccgcctgttc gcgggctggg cgccttgcgc catcgccatc gccgccggta ccgctttcaa
      121 gatcgcggac cggatcggca gccgggcggc tgccgtatat caggaccagt tcgaccgcga
      181 cggtgcggta gcccacgcct ggttgacccg gcgggggatc gaacttggcg ggccggtgct
      241 tcgtcagatt gctgacctgt tcgatgccag cccgctgcaa agcgactggc gatcctctgc
      301 catacccgaa cagcggcttg ccggcctggt tgccgatcca tcgatgccgg cggcacatcg
      361 ccgggacgca gccgaagcct tgcttcgatt ccgtgcccgg acggcggaaa tgccgccgtt
      421 ccctcgtctc gtcacgcggc ccctcggcat gctgatgctg gtgccgtgat gctcgatctg
      481 gaggactgca tcctggccgg ttcggcgatc accgcgccgt tcctgcgcga cgggctcgcc
      541 cgggtactgg acctggggcg cgatgccgcg gatggatggg aaagcgtgcg cctgtcgctg
      601 cgcatcggcg gggcggagcg cgtccatcgc catgtcacga tgaagctggc atcggctttc
      661 ggatacggcc ggccggtgcg ccaaagcgac gtcacgaccc gcgagggacc ggaagacggc
      721 ggctggctgc tgcgggcatc cggtggaaag cagttgcgcg cgtggtcgat ccccagcgat
      781 gtcgacctgg acgggttgga acaatcgccg cgtagctacc ggtcaagtcc aacccggatc
      841 gcgcaacgcg tcttgctggc ccgcggcgag cgcgccggac tgctgaccaa cggggaggta
      901 ctccgtctgc tgctgagcca tccatcccgt gcggatagtc acctgtcgat tggcatcggc
      961 ttgtggcgtc aggcgccact gccgccggat tccttccgcg tcttgaaggc gctggccggg
     1021 gtagatggcc tggctcggtt gcccgccgta ctggaagcgg cgcggctgca tcaggcgcgg
     1081 gtgacgacgg aactgcggcg ccaggcgaag gaagccatcg ttggtttcat caacgcgctc
     1141 cccagccgca cggggcttga tccggcggct ctgtggcatg agggcctggt tctggtttac
     1201 cggctgctgt tcattctgaa gctggaaagc ccggcggaag caggcggtgg gttcagtttt
     1261 gcctccaccc ggctgtggcg ggaggccctg tcgccaaacc gagcgctggg gccgttggtg
     1321 cgacggcgcc tggatcacgg gcacgacacg ggccggatgc tggaagccgg tttgcgttcc
     1381 ctgttcgtgg catttcgcga cgggctgcgc tgcagcgagc tgcatatcgc gcccctgggc
     1441 ggtgcgctgt tcggttccgg cacgacgccg ttgctcgatc gcctggattg gggcgaccaa
     1501 gccgtggcaa tcctgttgga ccggctgatc tggcttgttt cgccacgcgg cgagcgggtt
     1561 cgggtgcatt acggctcgct ggatgtggag gatctcggca gcatctacga gggattgctg
     1621 gagcagcagc cggggatcgc aacggaaccc ctgatccgcg tgcggcatgg caagacggag
     1681 gctgtggtgc ccgccgctcg tcaacaagcc gatatcgaga ccggtgcgtt ctttctgcgt
     1741 tcggggatgg gacgcaaggc ctcggggtcg ttctacacgc cgcacgactt cgtccgcttc
     1801 ctggtgcgcg aaacgctgga ttccaagatc gcgaccctga gtccgcccga cgatcctcag
     1861 ccggccaggc ttctaacgtt gaagatcgtc gatccctcga ccggcagcgg ccatttcctg
     1921 gtggaggctt gccgttattt gggggaggcg ctgctgaccg cctgccgcct gtgcgacaag
     1981 aagggtctgc acgaccgcat cgccgccctg cccgatccgg accagacgat cgcggcctat
     2041 ctccccagcc gaggattttc cgaagctcgg gcgcgcgcga tctgccggcg tctggtcgcc
     2101 gtgcattgcc tgtatggtgt ggaccgcaac aagctcgccg tcgaattggc aaagctgtcg
     2161 ctgtggctgg agtcctatgc cgagggattg ccgctgacct tcctggacca ccggctggtg
     2221 cacggcgatg cgctgtccgg gccattcttc gctgccttgg cgacgctgcc ggtgaccggc
     2281 ggggcgctcg atccgttgct ggcgcgtggc gtggccgacc ggctggattg cagcctcagg
     2341 caggcccgca tgttggtggc cgaactgaat gcatccatcg gccgcgacct gaccgatctt
     2401 gggacgaaac aggtggcgaa ggatcggttg gatcgattgc tgcatccgtt gcggcagctc
     2461 gcacgcgcct gggcgggtgc ggcgatgctg cgggaacgcg acagcgacga tgtctggctg
     2521 ggactggcga cccatgtcgc ggcaaagggt tgctggcccg gcactttgag cgggggtcag
     2581 gccgcgctgc tggccgcagg gtcggatgcg gtggcctggg atctggtatt cccggaggtg
     2641 tttcccgatg gtttctcggt cgtactgggc aatccgccct gggatgtgct gctgccgaac
     2701 accagggagt tcgtcgccga ttacgatccc accatattgg atgcacgcac gcgttcggaa
     2761 cgggccgcga tcgaacacgc ggcgttggcg caaccggccg tcgccgcggc attcagcgcc
     2821 taccgcgacg gcttcgagcg gatgaaaaac gtcgctcggc ggctgtatcg gcatcagcgg
     2881 gttcaggccg gcggggaaac aacgtcgggc aacatggatc tgtttcgcct gttcgcggag
     2941 cgcaacatgg acctggccgc gccgaccgga gcgattggcg tgctgatgcc gtcggccttt
     3001 catgccaatg agggcaccac cggcatacgg cggctgtatc tacaggaagc ggatctaagc
     3061 tggtgcctgt cgttcgagaa ccgtcgccgc atcttcgaca tcgacagccg cttcaagttc
     3121 gacctgatcg tggcgcaccg ccccggtccg acacggtcgt tgcgctgcgg gttctacctg
     3181 gaccgtatcg aggacatcaa cgatccccgc aagatcatgt gctacagtat ggcgtttctg
     3241 tacaggaccg gcggcgccgg tttggcgcca ttggaactac gtggaaacgt tgaccagcgg
     3301 attgccgaca tcctgttcgc acagcccgaa cgactgggcg cgtggtgcgc ggatcgccac
     3361 atccggttcg gctgcgatct gcacatgacc gccgatgccg gctgcttcca gcccgccggc
     3421 cagggcaaca cggtcctgta cgaaggcaag acgtttcacc agtacagcga cacgtgggac
     3481 tcgaagccgc gctacagcgt ggcgtcagag gcactcaagc cggcaatcgc cgaggccgcc
     3541 cggcattacc gcctggcgtt ccgcgacatt gcccgatcca acgatgaacg gaccatgatc
     3601 gcctgcatcg taccgccggg cgtggtgttc ggtcataccg cgaccgtgga gaaacatccg
     3661 ggcgcgcgct ccgtcgctga tgcgttggtt ttgtgcgcgc tgttcaactc gtttccgttc
     3721 gactggttgg tgcgccagaa gacagcgacg catctcagtc tttatatcct gcaggctctg
     3781 ccggttcctc ggtttggcga ggctgaaaac agctttttag cacaagcggc gctgcggttg
     3841 tcctgcaaca atgatggcta tgcgaaccta tggcgggatc aggtcggcgg ccgacaaacc
     3901 gcgttacaac agtgctggga gttgcgcgcc gccatcgatg cggttgtggc ggaggcatac
     3961 gggctggatc gcgatcaata cgaacatcta ctccgaagtt tcagccaccg ctccgatccc
     4021 ggggcgccat cgctctgcct tgccgcattc gatcggttgc aggcgatggg gcttgcagat
     4081 ttctgccccc atggtggaac cgaagcgtca gccggataac ccccgcagca agtccagcac
     4141 gcttgctatc tgccggtcca ggccgtagcg gtccctgacc cagtcagtat aaaggccggt
     4201 gcgagccgcg ccggtcaatg cgaccgcttc atcgatcgaa gcgaacaggc actcgtgcgg
     4261 ccagagccgg tctgcgccgg gaaatccatg caccacgggg aatgcgccgg ccgccatcgc
     4321 ctcgccgatg ttcatgccga agctttcgta catggacgtc gagagcagca cgcccttgtc
     4381 ggcgtaccag gccggcatgt cggcgacatg gccgttgaag tgcaccgcgc ccgccagccc
     4441 gagggcagat tgcatttgcc gcaggtagcg cgctgtccgc aggtcggtga aggctccggc
     4501 gacgtggaag ctgaaccgag cgtcacgctg ccgcaggcga tgcgcgatct gcatcagcag
     4561 catcggattt ttcttcggct ccagatgccc cacccagccg acgcggaagc gatccggaga
     4621 acccgggcgg aagcgggcgg tgtcgatacc attggggatg acttcgatcc gcaccgcgtc
     4681 ggcgatgccg ggatgcgcgg tcagcaagac gtccttgatg tcgtcggcga ccgtgatcag
     4741 ccgggacacc cgcgtccagt ccatccgcgc cgggtagtcc gtctgcaacg cctcgatcga
     4801 gtgcagccgc acgacacaga gcttacccgg tggcagcgcc tgccgtgtcg cccagaccgc
     4861 atggtcccag caccattcca gccagaccag atccgcccag ccgatcgcct cggccagccc
     4921 atcaccgggg cctgcggcga tgaagcgaac ttcatagtgc tgttcaagcg ccggtatcaa
     4981 aggggcaagg aaactgtcgg tccggccctt gacccggtct gcgagcaaca agcgtttcat
     5041 caaagcatcc gtagattccg ccggatgcta tgccgcgtcc ggttaaccaa agatgaacgc
     5101 gacggataac gctgatgtcg ttttcgatgc gccgcaacaa aagctgtaat tgcagtgctt
     5161 tgacacgtta caacgggcaa caatcattga ctcggcgcag ccggtggaac cagctaacca
     5221 agagccttgg tgcccgcgat cccacgggcg ccgtatggaa tgcccttgat ggacggcttg
     5281 acccagttgg tagttgacga atccggcgct tcctcggaat ggcggcaacg cactctgaag
     5341 acggcgatca gttgcgttgg cgtcggggtc cattccggcc gtcgcgtcaa cctgaccatc
     5401 cagcccgccg cagccgacca cggcatcgtt ttccgccgca ccgacctgga tcgcgtcatc
     5461 gaagcgcgct tcgacaatgt ctgcgacact cgcttaagca ccgtactcag cgatccggcc
     5521 atggcatccg cccgggtcgg caccgtcgag catctcatgg ccgcgctttc cgcgctcggt
     5581 atcgacaatg ccctgattga cgtggacggc ccggaaattc ccatcctgga cggttccgcg
     5641 gcgccgtttg tgttcctgct cgattgtgcc gggtgggtgg aacaacaggc tccacgccgg
     5701 gtcatcgaaa tccgccgtac cgttcgggtt tccgccggca acgcctgggc ggaattgcgg
     5761 ccgttgggcc cggccgtccg agcaatgcgg ccggtactgg aactggagct gtcgatcgac
     5821 ttcgccgcca gcgcgatcgg ccgccagtcc gcttccctgc gcctgacgcc cgacagtttc
     5881 cgccacgaga tagcttcggc ccgcaccttc gcgcaggcgg cggaggtcga gcagctgcag
     5941 gctgccggcc tggcgcgcgg cggcagtctg gacaacgcga ttgtcgtcga tggagcagac
     6001 attctcaatg ccggcggtct gcgcatggcg aacgagttcg ccagtcacaa gctgctggac
     6061 gcggtgggtg atttggcgtt ggccggcggc aagctgcatg ggcgcttcgt cgccagtcgc
     6121 accggccacg agttgaacaa tcgcctgttg cggtcgttgt tcgcggatga aaccgcctgg
     6181 gcctcggtgg tcaccgaacc tctcgtcgcc gccgcctgat ccaggcagtt ccagcatggc
     6241 tgcaagcgcg cgttggccag cgcgtgggaa caaacgcttt tccccgtatt cgcgaggccc
     6301 gagggcatga tatagggcgc gggatgaaca cgagtcgccc tacgtccttt cgctccgcac
     6361 gcggcctttt cctcggtgca accacgcttc tgccgctgct gtcaggctgc ggttcggcga
     6421 cggatgacag tgccaagaaa ggtccgtccg gaccggtgga ggaactgtac aacaacggcg
     6481 tggatgcttt gagcgcccgc cgcttctcca ccgccggcga ccagttcaac gcggtggagc
     6541 agaactatcc ctactcgaca tgggcggtga acgcccagtt gatgtccggc tactcgctat
     6601 acctgcagaa caaatatacc gaggcgatca gcacgctgga tcgcttcatc cagctgcatc
     6661 cggcgcatcg cgacgtcgcc tatgcctatt acctgcgcgc gctgtgctac tacgagcaga
     6721 tcgccgacat ccagcgcgac cagaagggca ccgaacaagc gatgaatgcg ctgcgggagg
     6781 tggtgacccg gtttccggac accacctacg cccgcgacgc caagctgaag atcgacctgt
     6841 gtttcgacca cctcgccggc aaggagatgg agatcggccg cttctatcag aagcagcatc
     6901 tgtatgaggc cgcgatcggc cgcttccagc gggtggtgga tgatttccag accaccaacc
     6961 atgtgcccga ggccttggcc cggttgaccg aggtttatct cgcgctgggg ttgaaggatc
     7021 aggcgcgcaa gacatccgcc gtcctgggct acaactatcc cggcagcgag tggtatgacg
     7081 ccagctacgg ccagctcgtg gacagtggaa ttgcctcccc ggccgacaga tcgccttccc
     7141 aggagcctga gcggcgtggt tttctctccc gaacatggca tgcggtgttc tgatcccacc
     7201 cgattagggt ctgtgggcga cgtagcatgc tgaccgcgct gtcgatccgt gacgtcgttc
     7261 tgatcgaacg gctcgacctg gcgttcgacc atggactgac cgtgctgacc ggcgaaaccg
     7321 gcgccggaaa gtcgatcctg ctcgacagcc tcggcctcgc ccttggtgcg cgggcggaaa
     7381 gcggcctggt tcgcgcgtcc gccgaacagg ccagcgtgac cgcgtgcttt tcatcgccga
     7441 accatccttc catcccgtcc atgctggacg agcatgggct gacagccgac gccgagatcg
     7501 tgctgcgccg cgtcgtcacc aaggacgggc ggtcgcgcgc gttcatcaac gaccatccgg
     7561 tcggtgtcgg actgctcagg cgcgtcggcg cgctgctggt ggaagtgcag ggccagcacg
     7621 agcaaatgag cctggccgac ccggccagtc atgccggtct gctggacgcg ttcggcgtac
     7681 ccgcggagtt gcgcatcggt gtttccgaaa cgtggcacgc ctggcggaat gcgctgcgcc
     7741 ggctggagca ggcccgcgaa gccatcgccg ccgccgaacg cgacgaggaa tggctgcgcc
     7801 acgcggtgga cgaactcgcg actttggcgc cgaggattgg tgaggaggaa accctggccc
     7861 gcgagcgcca gcggttgcag caaagcgaac gccgggccga ggcgatcgcc gccgctctgg
     7921 cggaactgac gccgcgagac cggcggaatg cggggccggc ggcttcgctg cgatcggcgt
     7981 cacgagcttt gcagcgcctg gtgccatccg gccagcccga ggcaggggga aatccggctg
     8041 acacggcgct tgccgcgctg gaaagggccg aggaagcgct ggccgaggcg gaaaccgtgt
     8101 tgtcgcgcct gatcgcggat gccgatgtcg atccgcgctt gctggaacag tcggaggaac
     8161 ggctgttcgc cttgcgcgcg atcgccagga agcacgctgt cgcggtgccc gacttgccgg
     8221 agttgctgga cactttggcg gctcggttga cggcactgga aaccggcgcg tccgaaatcg
     8281 cggcactgga gcaggcgacg cgggacacca aggaccggta catggccgcc gccgccgcgt
     8341 tgtccgccgc gcgagaagcc gccggcggga aattgcagaa ggccgtgggc aaggagctgc
     8401 cgccgctgcg cctggacaag gcccgctttg ttgtcgaagt gggcgcactg ccggaagccg
     8461 gctggggacc aggaggcatg gatcaggtaa ggttcctgat cgcgacgaac ccgggccagg
     8521 agccgggcgc tttggcacgg gtggcctcgg gcggcgagtt gtcgcggttg atgctggcga
     8581 tgaaagtggt gttgtcggcc ggttcggctg tgccgacact ggtgttcgat gaggtggatt
     8641 ccggcatcgg gggtgcgacg gccgcggctg tgggtgagcg tttggttcgg gttgccgagg
     8701 gggtgcaggt gctggtggtg acgcatagtc cgcaggttgc cgcacgcggg gccgcgcatt
     8761 tgcgggtcgc caaggccgct acccgcgatc gcaccgccac caccgtggat catctgggac
     8821 cagatgctcg gcgggaggaa atcgcgcgga tgctggccgg ggagatcatc tccgacgccg
     8881 cccgagcagc ggccgacgat ctgctgcgtg ggcaacggcc gcagcagggg ttgctggtgt
     8941 gatggctgct acggatccgc ggcgcaatag ccgcctcggg cgcggtcttg gattccgacg
     9001 tggttaggcc aatgcgacga tccgcgcgat gctagaagca acggcacgac aagccgtcga
     9061 gacgctgacg gaggcggaag ccgccgaaga actcgagcat ctggcgcggg aaattgcgca
     9121 ccacgacgct gcgtatcata cccatgatgc gccggagatt tccgacgccg actacgatgc
     9181 gctgcgacgc cggaattcgg caatcgaggc gcgttttccg gaactgatcc gggctgactc
     9241 tccgtccagc cgtgtcggcg gggcgcctga gcccggcttc gcgaaacttc gccaccgcgt
     9301 tccgatgctg tcgctcgaca acgccttcga cgcggccgaa tttgccgaat tttgcaaccg
     9361 agccaagcg
//
LOCUS       DASWQN010000147         3484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1071776, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000147 DASWQN010000000
VERSION     DASWQN010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071776"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2287
                     /locus_tag="VGC82_02440"
     CDS             <1..2287
                     /locus_tag="VGC82_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0422160.1"
                     /translation="VISSNIDIEQRFAYPDFVKDAGVKALVNVIIIGSEGKPPYGILQ
                     VDSRTPREFSEGDTNFLRSYANLLAAAVERLRIKAEADRVQALGRESETRYRAIVESA
                     SDYAIITLDPQGRITGWNRGARTIFGWEEADVLGQPTGLIFTLEDRAANMPAKEMRKA
                     RATGRAEDERWHVKRDGTPFWASGQLTLLDEGGLHGYLKILRDHTEQRVVAERLRTSE
                     ERFRSLAEGIPQLVWRSRSSGERTWGSPQWIVYSGLSEGESLGFGWLDAVHPDDHATT
                     MAAWAKAEASGEFAAEYRVRQAADGSYRWFQSRATPVHDAIGHIVEWLGTSTDIDEQV
                     RAREVLARGREELERLVAARTSDLALALDSLRAEAAERGNAEEALRQSQKMEAVGRLT
                     GGIAHDFNNMLQGIAGSVEIARRRIGEGRNNEALRFLNTTRVAVDRAAALTHRLLAFA
                     RRQRLDPRPVDPDGLIAGMAELIRRTMGPGIRVELNLRDGVWSVLCDPSELESALLNL
                     CINARDAVPDGGKLIIGTADKHFGPEEAAEHEEASPGEYIAITVTDTGEGMPPDVMTR
                     VFEPFFTTKPLGQGTGLGLSQIYGFVRQSKGVVQIQSALGQGTTVRISLPRHERATEQ
                     LEQASSPEPEQARVGETVLLVDDEDGVREPASEYLRELGYNVLEAEDGFAALRILESI
                     SRLDLLVTDVGLPNGMNGRQVAEAVRQQRPRLPVLFITGYAGTALEPGTEVVRKPFDL
                     DAFARRVQALLPNQGAASGAR"
     gene            2460..3326
                     /locus_tag="VGC82_02445"
     CDS             2460..3326
                     /locus_tag="VGC82_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEY0422161.1"
                     /translation="MYFHDTRLQYPVRVTTPDPLFARQLQQAIGGIEGEIRVCLQYFF
                     QAWGARGPTNKYRDMLLHTATEEIGHIEMLATAVALNLENAPATLQESAVQSSSLVSA
                     VMGGGERPRHMVEGMLQKHLLSTGMAAFPGNSDGLPFDCSHIYASGNLAADMFSNVAA
                     ESTGRLLAVRLFNAAHDEGMRDMLSFLIARDTMHQQQWLAVVEELGGAAALPIPNSFD
                     QSQEKQEFSYVFLATNREGIPPAVGRWNEGPSLDGKGTFTARQNEPLGQEPVLGPARP
                     DSGAQREQMEER"
BASE COUNT          673 a         1062 c         1158 g          591 t
ORIGIN      
        1 ggtcatctcc agcaatatcg atatcgagca gcggttcgcc tatcccgact tcgtcaagga
       61 cgccggcgtg aaagccttgg tgaatgtgat catcatcggc tcggagggta agccgccata
      121 tggcatcctg caggtggaca gtcgcacccc acgggaattc agcgaaggcg acaccaactt
      181 cctgcgcagc tacgccaact tgctcgcggc ggcggttgag cgcctgcgta tcaaagccga
      241 ggcggaccgt gtgcaagctt tgggccggga gagcgaaacc cgataccgcg cgatcgtgga
      301 aagtgcgagc gactatgcca tcatcaccct cgatccgcag ggacggatca ccggctggaa
      361 ccgcggcgcg cggactatat tcggctggga agaagcggat gttctgggac aaccgacagg
      421 cctgatcttc accctcgagg atcgcgccgc caacatgcct gcgaaagaaa tgcgaaaggc
      481 gcgggccact ggtcgtgccg aggacgagcg gtggcatgtg aagcgtgacg gcacgccgtt
      541 ttgggcaagc ggccaactga cgctgctgga cgaaggcggc ctgcacgggt acttgaagat
      601 cctgcgggat cacaccgaac agcgtgtcgt agcagagcgt ttgcggacca gcgaagagcg
      661 cttccggtcg ctggctgaag gtatccccca gctggtttgg cggtcccgca gttccggcga
      721 acgtacttgg ggcagcccgc aatggatcgt ctattcaggc ctgtccgagg gggaaagcct
      781 gggcttcggc tggcttgatg cggttcaccc cgacgaccac gccacgacga tggctgcctg
      841 ggccaaagcc gaggcatcgg gcgaattcgc cgccgagtat cgcgtacgcc aggcagcgga
      901 tggtagctac cgctggtttc aaagtcgcgc cacgccggta cacgacgcaa ttgggcacat
      961 cgtcgagtgg ctgggtactt cgaccgatat cgacgaacag gttcgtgccc gcgaggtgct
     1021 cgcccgcgga cgtgaggaac tcgaaaggct cgtggccgcg cggaccagcg acctggcgct
     1081 tgcactggac tcactgcgcg ccgaggcggc cgaacggggc aacgccgaag aggcgctgcg
     1141 ccaaagccag aagatggaag cggtcggccg gctaaccggt ggcatcgcgc atgatttcaa
     1201 caacatgctc cagggtatcg ccggcagcgt cgaaattgcg cggcgtcgga tcggcgaagg
     1261 gcgcaacaac gaggcgctgc gcttcctcaa cacgacgcgg gtcgcggttg accgggcggc
     1321 ggccctgacc caccggttgc tggcgtttgc ccggcgccag cggctggacc ctcggccggt
     1381 ggatcccgac ggcctgatcg ccggaatggc agagttgatc cggcgcacca tgggaccggg
     1441 aatccgggtg gagttgaacc tgcgcgacgg cgtctggagc gtgttgtgcg atccaagcga
     1501 gctggaaagc gctttgctga acctttgtat caatgcgcgc gacgccgtgc cggacggcgg
     1561 caagctgatt atcggcacgg ccgacaaaca tttcggtccc gaagaagccg cggagcacga
     1621 ggaagcctct ccaggcgagt atatcgcgat cacggtcacc gacaccgggg aaggaatgcc
     1681 gccggacgtg atgacgcggg tattcgaacc gtttttcacc accaagccgc tgggccaggg
     1741 aaccggcctt ggcctcagtc agatctacgg ttttgttcgg cagtcgaaag gcgttgtcca
     1801 gatccaaagc gcgctgggac agggcaccac ggtacggatc tccctgcccc ggcacgagcg
     1861 ggcgacggag cagcttgaac aagcttcgtc gccggagccg gagcaggcgc gggtcggcga
     1921 aacggttctg ctggttgacg acgaggatgg cgtgcgcgag cccgcctccg agtatctacg
     1981 ggagctcggc tacaacgtgc tggaagccga ggatggtttc gccgcgctgc ggatcctgga
     2041 atccatctcc cggctggacc tgctggtgac ggatgttggc ctgcccaacg ggatgaacgg
     2101 gcgccaggtg gcggaagcgg tgcgccagca gcggccacga cttccggtgc tgttcatcac
     2161 cggctatgcc gggacggcac ttgagccggg gaccgaggtg gttcgcaagc cattcgacct
     2221 cgacgcgttc gccaggcggg ttcaggcgct tttgcccaac caaggtgccg catccggcgc
     2281 gcgctgacgg cgccgccgct tgccgattaa ttcttatcag taatatcaat ttgaacgaca
     2341 gataatccgg cgctcgcggg aacgtgccgg ccatgccgct gcgccccggc cgaaccgcag
     2401 cgaatgacac tcgtttgcag tttgtccgcg gctcttcgcc gacactcaag gagcattgaa
     2461 tgtacttcca tgatacacgg ctgcagtatc cggttcgggt caccacgcca gatcccttgt
     2521 tcgcgcgcca acttcagcag gcaatcggcg gcatcgaagg cgaaatacgc gtttgcctgc
     2581 aatacttctt tcaagcgtgg ggcgcgcgcg gtcccaccaa caagtatcgc gacatgctgc
     2641 ttcataccgc gacggaggag atcgggcaca tcgagatgct ggcgacggcc gtcgcgttga
     2701 atttggagaa cgccccggcc acgctgcagg aatccgcggt gcagtcgagt tcgctggtca
     2761 gcgcggtgat gggtggcggt gaacggccgc ggcacatggt ggaaggcatg ctgcagaagc
     2821 atctgttgtc cactggaatg gcggcgtttc cgggtaactc cgacggcctg ccgttcgact
     2881 gttcgcacat ttacgccagc ggcaatctcg ccgccgacat gttctccaac gtagccgccg
     2941 agtcgaccgg ccgcttgctc gccgtgcgcc tcttcaacgc cgcgcacgac gagggcatgc
     3001 gggacatgct gagcttcctg atcgcgcgcg acacgatgca ccagcagcaa tggctggcgg
     3061 tggtcgagga actcggtggc gccgctgcat tgccgattcc gaacagcttc gaccagagcc
     3121 aggaaaagca ggaattcagc tacgtcttcc tcgccaccaa ccgcgaaggc attccgccgg
     3181 ccgtcggccg ctggaacgag ggaccgtcgc tcgacggcaa gggaacgttc accgcccggc
     3241 aaaacgagcc gctgggtcag gaacctgtac ttggcccggc tcgcccggac agtggcgcac
     3301 agcgcgagca gatggaagag cgctgaaagc agggctctct ccgcggcagt tgatagttgt
     3361 gttgccgtgc ctggcgcggc cacccacgac ttttcttaac agggaaagtc gtgggtggta
     3421 cagccgccgg taaagaccca agcggcaaag acattcgatc gcgatcagag cgttttcacg
     3481 ctga
//
LOCUS       DASWQN010000148         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_148436, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000148 DASWQN010000000
VERSION     DASWQN010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148436"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VGC82_02450"
     CDS             complement(<1..694)
                     /locus_tag="VGC82_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422162.1"
                     /translation="MPITRRTALSAAASSLLLPAVRTRAQSRPVIRIGVINDMSGTYR
                     DQTGPTGVACAKQAMQEFNTSGAFAVEVLSADHLNKPDVAVSIARKWFDQDGVDILLD
                     CAASSAALAMASLCKEKNKVMIATSTATSDLTGKACTPNSLHWVYDTYMVAKSTGTAT
                     VKAGGNKWYFVYPNYAFGEALHRDTSRFVKEAGGIIAGAETYAFPETHDFSSALIKAR
                     ASGANVLGFCGSG"
     gene            1272..>1764
                     /locus_tag="VGC82_02455"
     CDS             1272..>1764
                     /locus_tag="VGC82_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422163.1"
                     /translation="MTDKPKKLSATARMLLTTAAMRADHLIHPPQLPIAAARQVARSL
                     LGSGLAEEMPTPDKAADFAWPVGDGGRSLMLRATAIGLARIADRANVTAASVANDAMA
                     EAATRLTEAGSDDGNTATALAPTTGQQSSASACHPDQDAQAAERAPETPRRARHPDLL
                     WEAA"
BASE COUNT          344 a          555 c          539 g          326 t
ORIGIN      
        1 tgcccgatcc acaaaacccg aggacgttgg cgcccgaggc gcgagccttt atcagggcac
       61 tggaaaagtc gtgggtctcg ggaaaggcgt atgtttctgc tcccgcaatg atgccacctg
      121 cttctttcac gaagcgcgac gtgtcccgat gtagcgcttc gccgaaggca tagttgggat
      181 atacgaaata ccatttgttg ccgccggctt tcaccgtcgc ggtgcccgtt gatttcgcta
      241 ccatgtaggt gtcgtacacc cagtgcagcg agttcggtgt gcaggccttg cccgtaaggt
      301 cggacgtcgc cgtgctggtc gcgatcatta ccttgttctt ctccttgcag agcgacgcca
      361 tagccaacgc ggcggaagag gcggcacagt ccagcaggat gtccactccg tcctgatcga
      421 accacttacg cgcgatgctg accgcgacgt cgggcttgtt cagatgatcg gccgacagca
      481 cctcaacggc gaacgcgcca ctggtgttga actcctgcat tgcctgtttg gcacaggcca
      541 cgccggtggg accggtctgg tcgcgatagg tcccggacat atcgttgata accccaatgc
      601 ggatgacagg cctgctttgc gcacgagtgc gaaccgccgg cagaagcaac gacgaggccg
      661 cggccgacag tgcagtccgg cgggtaattg gcatggttgc aggtccttgt gacgctttcc
      721 ctatcatagt gtcgccttat ggtcgcggcc gcacaaggcg gccatggcga tgacgttcaa
      781 gagcacctcg gtgcagatct tgcttccggc gggaaaaacc gttctgccga gacagcgacg
      841 gtatcgctaa ttgcagagcc tgctgtctgg cgcatgtctg caataacgtc cggcgcctac
      901 gatccgcgat cgcggtagcc acgaaagatc aatgagatga tcagcttatt cgcttggctg
      961 cgccgcgatc cagagcatgt atactcagcg acagcgggct gatcctaccc agtggaacca
     1021 cagacaacgg cacagcggcc accttatctg attgcaggac ttgaaaatct gagcgttcgg
     1081 cgcggcggcc ggatacgaca tcgaccttgg acgagcgcga cttcttggca ctcgcccgga
     1141 tgaccgcagc aggtgaacat cgccgcctgt cacatggatt gcgaccccgc ctttatgctc
     1201 cgaccgaact cagcccggcg gggctcggcc tggtagcggt gcctgatcgg cccgcttcct
     1261 ggagttccat catgactgac aaaccgaaaa aactttccgc cacggcgcga atgctgctga
     1321 ccacggcggc gatgcgcgcc gaccacctga tccatcctcc acagctgccg atcgctgcgg
     1381 cacggcaggt tgcgcgatcg ctgctgggtt ctgggctggc cgaagagatg ccgacgcccg
     1441 ataaggcggc agactttgca tggccagttg gtgacggcgg cagatcgctc atgcttcggg
     1501 cgacggcgat cgggctggct cgtatcgccg accgcgccaa cgtaacggcc gcctcagtcg
     1561 cgaacgacgc aatggctgag gcggcaacgc gcctgacgga ggctggatcc gatgacggca
     1621 acacggcaac cgcgctggcg ccaaccacag gacaacaatc gtcagccagc gcgtgccacc
     1681 cagatcagga cgctcaggct gccgagcgag cgcccgagac tcctcggcga gccagacacc
     1741 cggacctcct ttgggaagcc gctc
//
LOCUS       DASWQN010000149         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1335828, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000149 DASWQN010000000
VERSION     DASWQN010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335828"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VGC82_02460"
     CDS             complement(<1..947)
                     /locus_tag="VGC82_02460"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422164.1"
                     /translation="MRSNRQEPAVFVASDDATLNGLLGALPIFLPDHRILALPAWDSL
                     PYDRTRPSRPNTGTRVASLAWLADAHDQPALVLTTPEALPQRIPLPERLRERSVRLTT
                     GDQLDPAWLRATLEAFGYALEERVDGPGEAALRPGAVDIFPAGQTDPVRLELDEGRIV
                     AIRPFDPLTQRGTGDSGPLHLLPASEAFTVPGEADPAALTRTAERLLPDGALASLFDL
                     IPGAALGLMQDTEDRIADWYALIRESYAVALDAVRAGHQAPPVPDTLFLPEAELAAEL
                     TRRGNVRFIMDAEQAGAALPPGPAVRRAASCLEKSEAVIL"
     gene            complement(1242..>1715)
                     /locus_tag="VGC82_02465"
     CDS             complement(1242..>1715)
                     /locus_tag="VGC82_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0422165.1"
                     /translation="GLGYVPEERRIFADLTVTENLEVGRQPPRAGRAPWTEQRLFDLF
                     PNLGRMRARLGGRMSGGEQQMLTIARTLMGNPTLLLLDEPSEGLAPVIVEEMAHAILA
                     LKRDGVTILLAEQNLHFAARVAGRAAVIEKGRIVWTGTMPELQADDAARAAYLAV"
BASE COUNT          286 a          533 c          597 g          299 t
ORIGIN      
        1 agtatgaccg cttcggattt ttccaggcat gaggccgccc gccgcacagc ggggccaggc
       61 ggcagagcgg cccctgcctg ttcggcatcc atgatgaatc gcacattgcc gcggcgcgtc
      121 agttcggctg cgagctccgc ctctggcagg aacagcgtgt ctggcaccgg cggcgcctga
      181 tggccggccc gtaccgcatc gagcgccacg gcataacttt cgcgtatcag tgcgtaccag
      241 tcggcgatgc gatcttcggt atcctgcatc agcccaagtg ctgcgcccgg tatcaaatca
      301 aacaggctgg cgagtgcgcc gtccggcaac aggcgctctg ctgttctcgt taacgcggct
      361 gggtccgcct cgccgggaac ggtaaacgcc tcgctggctg gcagcaggtg aagcggaccc
      421 gagtcgcctg tcccacgctg agtcagcgga tcaaacgggc ggatcgcaac gatgcgtcct
      481 tcatccagtt ccaggcgcac cggatcagtt tgccctgccg gaaagatatc gaccgcgccc
      541 gggcggagcg cagcctcacc tggcccatcc actcgttcct caagggcata cccaaacgcc
      601 tccagcgttg cgcgcaacca tgccggatcc agctggtcgc ctgtcgtgag gcggaccgaa
      661 cgttcccgca gccgttccgg gagcggaatt cgctgcggaa gcgcctccgg cgtcgtcagt
      721 acgagggcgg gttggtcatg cgcgtcagcc agccaggcca gcgaggcaac gcgcgtgccg
      781 gtgttggggc gggacgggcg cgtccgatcg tagggaaggg aatcccaggc tggcagcgca
      841 agaatgcggt ggtccggcag aaagatcggc aacgcaccca gcagcccgtt gagggtcgca
      901 tcgtctgagg cgacgaagac ggcaggttcc tggcggttac tgcgcaccca tctggcaatc
      961 gcgagcggga gcactccttc agtgatatgg acattggctg gcgcagcctc ggcgcgtgcc
     1021 ggagcgggtc cgatggtcgt cgtgttgttt tgcatctaat cggtcaggtg gtcttgccgg
     1081 caagagatca tctatcagca tgaacaacgc cccacctgct ggtcgttgct tcacgacatt
     1141 ggcacctggt caatcccaag cgattgtcca tgaaattccg ggcggcttgg ctggcgaacc
     1201 gtgacaggcg cggtgggatt ggcttgaccg aggcggatac cctaaaccgc cagataggcg
     1261 gcgcgagcgg cgtcatcggc ttgtagctct ggcattgtgc cggtccagac gatgcggcct
     1321 ttctcgatga ccgcggcgcg gccggcaacg cgggcggcga aatgaaggtt ctgttccgcg
     1381 agcaggatgg tgactccgtc gcgcttcagg gcgaggatgg cgtgggccat ttcttccacg
     1441 atgacggggg cgaggccctc actgggttcg tcgagcagca gcagcgtggg gttgcccatc
     1501 agcgtgcggg cgatggtcag catctgttgc tcgccgccgc tcatgcggcc gccgaggcgg
     1561 gcgcgcatgc ggccgagatt agggaacagg tcgaacaggc gctgctcggt ccacggggcg
     1621 cggccggcgc gggggggctg gcgaccgacc tccaggttct cggtcacggt caggtcggcg
     1681 aagatgcggc gttcctcggg gacgtagccg aggcc
//
LOCUS       DASWQN010000150         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_544350, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000150 DASWQN010000000
VERSION     DASWQN010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544350"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1197
                     /locus_tag="VGC82_02470"
     CDS             163..1197
                     /locus_tag="VGC82_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010505528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY0422166.1"
                     /translation="MGEVITIGLDIAKSVFQVHGVDVAGAVVIRKRVSRAKVLEYFGE
                     LPRCLVGIEACPSAHHWGRELQAFGHTVRLIPPSYVKAYLKRSKNDANDAAAICEAVT
                     RPSMRFVPIKTTEQQTALMLHRTRQLLVRQRTMLSNALRGHLAELGIVSAKGRNGTAE
                     LLRIIADGEDSRVSPAVRGILDVLARQYDAVGAEIASIDKSIISLHRACEASRRLAEI
                     PGIGPIGATALVAEIGDWNTFSSGRSLAAWIGLVPKQHSTGGKDRLGSITKQGNRYLR
                     WLLVVGAMAVIRYARKHGTERRPWLARLMERRPTKVAAVALANKIARMAWAIMVRGER
                     FKEPKLSLAA"
BASE COUNT          416 a          568 c          653 g          413 t
ORIGIN      
        1 cacttggccg ctttggcgga tgtccgctgt tagtggtcaa gcggaccttg agcaatcgcg
       61 gcttgaggtc agtttttgat gctgtgggcg gcgcccactc tgcggcatcg aggtgccata
      121 gggtggttgc gttagagcaa accacattga tgggagccgt ccatgggcga ggttatcacg
      181 attggtctcg atattgcgaa gtcggtgttt caagtgcacg gagtcgatgt tgcgggcgcg
      241 gtcgtgatcc gcaagcgcgt cagtcgcgca aaagtgctgg agtattttgg cgagctgccg
      301 cgttgcctgg tcgggatcga agcatgtccg tcagcgcatc attggggccg cgagcttcaa
      361 gcgtttggtc atacggtcag actgatacct ccgagctatg tgaaggctta cctcaaacgc
      421 agcaagaacg atgcaaatga tgcggctgcg atctgtgagg ctgtgacgcg gccttcgatg
      481 cggtttgtcc cgatcaagac gacggagcag cagaccgcct tgatgctgca ccggacccgg
      541 caattgctgg tgcgccagcg tacaatgttg agcaatgccc tgcgcgggca cctggccgaa
      601 ctcggcatcg tgtcggccaa aggccgcaat ggaacggcgg agttgctcag gatcatcgcg
      661 gatggtgagg atagccgggt gtcgccggct gtgcgcggta tcctcgatgt gttggcgcgg
      721 cagtatgatg ctgtcggcgc cgagatagca tccatcgaca agagcattat ttccctgcac
      781 cgggcctgtg aggcgagccg gcggctggcg gagatcccag gcatcggccc gatcggtgcc
      841 accgcactgg ttgccgagat cggtgactgg aatactttct cgtcggggcg cagtcttgcg
      901 gcctggatcg ggttggtgcc gaagcagcac agcaccggtg gcaaagacag gctcggcagc
      961 atcaccaagc agggcaatcg gtatttgcgg tggctgctgg tcgtcggggc aatggccgtt
     1021 atccgctacg cgcgtaagca cggcaccgaa aggcggccgt ggctcgcacg attgatggag
     1081 cgtcgaccca ccaaggtggc ggccgtagcg ctggccaaca agattgcgcg gatggcctgg
     1141 gccatcatgg tccggggcga gagattcaag gaaccgaagc tgtcgctggc ggcatgaggc
     1201 aggccagcag ctaatcggca acaccaattg gcgagggcat gacgacgtaa tgcagatacg
     1261 gtcgttccgg ggatcgggag aacccgcttg ggtcaacgca caccttctaa gtgcgcgttt
     1321 ttgtttggga cccgatccgc gcagagcatt agggccagcg gccatacctg ccgcatcaac
     1381 aggccggaca cgtgaccgca cctgaccaat gctgcaaaac gtcagaaagt tcttgccagg
     1441 gcgccgtcca cacgtgaccc aaaaccgaca tcgaggacca agaacgttca tcagattgat
     1501 gcacctcacc ttcgacacct cgccactggc gctcgttgta ttcctgatag ttctttgact
     1561 tgcatcgggc aggtcttaca cgtagatggg tgattgcatg tggatcatct ctcacgcgcc
     1621 gacctccgca accgtggctg ccaccgtcgt gctgttagtt tgcaatcaga cgcaaggatt
     1681 ggctcggggc agccaggttg gacacgacga cccctgggac gtggaacgaa tcaatcgtct
     1741 gcctgacgag gttcggaccg ctgttattcg catgtgccga actacgccgc gggccggtca
     1801 ctactttgcg acgtatatag acaactcacg cgtaatgaag ctgcacttcg aacatctgca
     1861 ctgtggtgga cagactccat tttgcaagga tcgtacttgc ttgcgccagg aatatgtctc
     1921 cacgggaggc cactatcagc tcaagaagag ctactacggc ggcaatgacg actgagcgcc
     1981 ggggggtgca ttacccccgg ccagccgcga gttcctcatg atccgaaacc gacacggatt
     2041 cctgataaag
//
LOCUS       DASWQN010000151         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2391647, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000151 DASWQN010000000
VERSION     DASWQN010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2391647"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VGC82_02475"
     CDS             <1..796
                     /locus_tag="VGC82_02475"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY0422167.1"
                     /translation="AVALLGMLTGYGLSLWAVPASMADFKEFQWEIRNRLAAFLLQDG
                     VFTPLSDKLTVYVRSRDQEGNLLGILVDDARDPTAHATILAERGRMLDGPTGPRVVLL
                     DGSRQEIDHQTGRLDILTFKQNEIDLAEATKGEAARPIDMSELPLASLLDPHPFFERD
                     RAKWKAEGHKRLTMPLTTMSYAMVGLFSALGGIFRRHGGVVRPLVTVGVMVGLLALGL
                     AFGTLAARDSDLLFLMWLHATVPGVVCAWLLLGPKLRSYQTQSAEA"
     gene            801..1913
                     /gene="lptG"
                     /locus_tag="VGC82_02480"
     CDS             801..1913
                     /gene="lptG"
                     /locus_tag="VGC82_02480"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY0422168.1"
                     /translation="MTAAITLSLYVVRQFALSVVSMLLALAGLVAMFDFIELLRRSAS
                     HPEVSFGMVAQIAALRLPYVAMQILPFAILLGGILCFWRLTRSSELIAARAAGISAWE
                     FLTAPTLCALLFGVIATALISPVSSVMLARAEAMDNAYLRTGGGPLALNGGQLWLRQS
                     DRQTSPRGVAIIHAQQVELHGKQLTAQQVSVFRLDESDKLLSRIEAARATLGSGSWRL
                     EDARTIRPDQLPEPPELLSLPTDLTVSRVQESFASPDTLSFWALPGFIELLDRSGFSS
                     IRHRLHFQALLALPLLAATMTLVSAGFSMRPARRGGVAKMIGSGVAAGFALFVVSKIA
                     EEIGQSGALPVVLAAWAPAASGLMLAVALLLHTEDG"
     gene            1961..>2267
                     /locus_tag="VGC82_02485"
     CDS             1961..>2267
                     /locus_tag="VGC82_02485"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEY0422169.1"
                     /translation="MITRSLTTLAVLLALGNITCTRPARAQMGVLTNPGAGHTLDKSE
                     PITFSADEVDYDKDKALVTATGHVEAWQNGHVMRADKVTFDRNTGVAAATGHVVMLEP
                     "
BASE COUNT          352 a          744 c          728 g          443 t
ORIGIN      
        1 agcggtggcg ctgctgggga tgctgaccgg ctacggcctc agcctgtggg cggttccggc
       61 ctcgatggcg gacttcaagg agttccaatg ggaaatccgc aaccgtctag ccgctttttt
      121 gctgcaggac ggcgtgttca ccccgctatc cgataaactg accgtttacg tccgttcgcg
      181 cgaccaggaa ggcaacctgc tcggcatcct ggtggacgat gcgcgcgacc ccaccgccca
      241 cgccacgatc ctggcggagc gcggacgcat gctggatggc ccaaccgggc cacgcgtggt
      301 gcttctggac ggcagccgcc aggagatcga ccatcagacc ggccggctcg acatcctgac
      361 cttcaagcag aacgagatcg acctcgcgga ggccaccaag ggcgaggctg cacgcccgat
      421 cgacatgtct gaattgccgc tggccagcct gctcgatccc catccatttt tcgagcgcga
      481 ccgggcgaaa tggaaagccg aggggcataa acggttgacc atgccgctga ccacgatgtc
      541 ctacgccatg gtcggtctgt tctcggcgct cggcggtatc ttccgacgcc acggcggcgt
      601 cgtccggccg ctggtcacgg tcggagtcat ggtcgggttg ctcgctttgg gcctggcctt
      661 cggcacgctc gctgcccggg acagcgacct gttgttcctg atgtggctcc acgcgacggt
      721 gccgggcgtc gtgtgcgcct ggctgctgct gggcccgaag ctgcgctcgt atcaaacgca
      781 atcggcggaa gcctgaccgc atgacggccg ccataacgct ttcgctctat gtcgtccgcc
      841 agttcgcgct ctcggtcgtc agcatgcttc tggcacttgc cggcctggtg gcgatgttcg
      901 atttcatcga actgttgcgc cgatccgcca gccaccccga ggtcagcttc ggcatggtcg
      961 cgcagatcgc cgcgctgcgg ctgccgtatg tcgccatgca aatcctgccg tttgcgatcc
     1021 tgctcggcgg gatcctgtgc ttctggcggc tgacccggtc ctcggaactg atcgccgccc
     1081 gcgccgccgg gatctcggcg tgggagttcc tgaccgcacc gacgctgtgc gccttgctct
     1141 tcggtgtgat cgccaccgcc ttgatcagcc cggtttcgtc cgtgatgctg gcgcgcgccg
     1201 aggcgatgga caacgcctat cttcgcaccg gcggcggtcc attggcgctc aatggcggtc
     1261 agctctggct gcgccaatct gatcggcaga cgtcgccacg gggtgttgcc atcatccatg
     1321 cgcagcaggt ggaactgcat ggcaagcagc tgaccgccca gcaagttagc gtgttccgtc
     1381 tggacgaatc cgacaagctg ctttcccgga tcgaggccgc ccgtgctacc ctggggagcg
     1441 gcagttggcg gctggaggac gcccgcacga tccgccccga ccagctgccg gagccaccgg
     1501 aacttctgag tttgccgacc gacctcacgg tttcccgggt gcaggaaagc ttcgcctcac
     1561 ccgacacgct gtcgttctgg gcgctgcccg gctttatcga actgcttgac cggtcgggtt
     1621 tttcatcgat tcgccatcgg ttgcatttcc aggcgctgct ggccctgccg ttgctcgcgg
     1681 cgaccatgac cctggtatcc gccggcttct ccatgcgacc ggctcggcgg ggcggcgtgg
     1741 caaaaatgat cggtagcgga gtcgccgccg gattcgcgct gttcgtggtg tccaaaatcg
     1801 ccgaggaaat cggccagtcg ggcgcgctgc ctgttgtact ggcggcttgg gcgccggcgg
     1861 catccggcct tatgctcgcc gtcgccctgt tgctgcatac cgaagacggc tgaggatgga
     1921 tcacgtggat tgttgttcgc aacgaggcac cttgctggcg gtgatcacgc gctcactgac
     1981 taccctggcg gtgttgcttg ccttgggaaa catcacctgc acaaggccgg cacgcgcgca
     2041 gatgggtgtg ttgaccaatc ccggagccgg tcacacgctc gacaagagtg agcccatcac
     2101 gttttccgcc gatgaggtgg actatgacaa agacaaggcc ctggtcaccg caacgggcca
     2161 cgtcgaggca tggcaaaacg ggcatgtgat gcgtgccgac aaggttacat tcgaccgcaa
     2221 caccggcgtg gccgccgcca ccggccatgt ggtgatgctg gagcccg
//
LOCUS       DASWQN010000152         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1468311, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000152 DASWQN010000000
VERSION     DASWQN010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468311"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..355)
                     /locus_tag="VGC82_02490"
     CDS             complement(119..355)
                     /locus_tag="VGC82_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422170.1"
                     /translation="MSQSSKPSKGTMRAVLHETEPGLYRAEYSGELNPEDSDARDFPD
                     SHIGTDADGVRQWVETMATNLGYDKVVWDSLDQA"
     gene            complement(414..542)
                     /locus_tag="VGC82_02495"
     CDS             complement(414..542)
                     /locus_tag="VGC82_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422171.1"
                     /translation="MAQTVEDFIRDRLYQPGVRRILGNPGDGIDGLFGMATPRKAA"
     gene            844..1614
                     /locus_tag="VGC82_02500"
     CDS             844..1614
                     /locus_tag="VGC82_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0422172.1"
                     /translation="MRQTLVLCLAVVLPLQAAWGGCAPVALGEPHVWRAALLDDVVEI
                     TFLGHASFAIVSPAGVRAVTDYNGINIPVDPPDIATMNHAHSTHYTLDPDQRIAHVLH
                     GWAEGGRTPAVDLTVRDMHVSNLPTNIRTWGGETELYGNSIFVFETGGLCIAHLGHLH
                     HLLQPADLNTLGHIDVAMVAVDGGYTMSQQDAAAVVEQLQPRIVLPMHYFTRGVLERF
                     LDLERGTYVIDVRDSPEMELSRTSLPSAPTVIVLPGGY"
     gene            complement(1639..>1819)
                     /locus_tag="VGC82_02505"
     CDS             complement(1639..>1819)
                     /locus_tag="VGC82_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422173.1"
                     /translation="DPSLAEGWWAVERQGSSIRRWTNGNALLPLGGVAGATMLEIHVS
                     GDMRYVAKPKERDAA"
BASE COUNT          319 a          552 c          567 g          381 t
ORIGIN      
        1 gcggtttcgc ccgctcgttc tcccttggcc agcgcaacat ttgccccccg accgtgggca
       61 tggccactac agccagcgct gatccggtca tctgaccgag gccgatagga tcggccgctc
      121 aggcctgatc gagtgaatcc caaacaacct tgtcatagcc cagattggtc gccatcgttt
      181 ccacccactg cctgacgccg tcggcatcgg tgcctatatg ggaatccggg aaatcgcggg
      241 cgtccgagtc ttcaggattg agttcgccgc tgtattccgc ccggtacagc cccggttcgg
      301 tctcgtgcag caccgcgcgc atggtgcctt tcgacggttt cgacgactgg ctcatggtgt
      361 tagctccgtt tggggactaa tatggatggc gagttccttg ggggtgccga tgatcacgct
      421 gccttcctcg gggtcgccat tccgaacaga ccatcgatcc cgtcgcccgg attgccgaga
      481 atgcggcgaa cacccggctg gtagagccgg tcccggatga aatcctcaac agtctgcgcc
      541 atctccgttc cctgcttact gcgatgcggc gggaaccgtc ccggagcagc ggcggttccc
      601 cggatagcgg ctaattcatg cggtatttgt cttggctggt agaccgcgcc tcgggatcgg
      661 cgagagcgtg atcacagtgg gcggaacgtc cgaaccgagt gaatcacgcg ataagacaag
      721 gagatagatc atttgcggcc tgcacgatca ggctggggat ggtctaccaa caggccagct
      781 tcggtcagtt gaagcgatgg ccgcatggcc gcatgatcgt ccggacctga aggatgctgg
      841 cggatgcgac aaacccttgt gctgtgtctt gcggtcgtcc tccctttgca agcggcctgg
      901 ggcggatgtg cgccggtcgc gctgggcgag ccccatgtct ggcgagcggc gctgttggac
      961 gatgtcgtgg agattacctt cctgggacac gccagtttcg cgatcgtttc gccggccggc
     1021 gtccgcgccg tgaccgacta caatgggatc aacattcccg tcgatccgcc ggatatcgcg
     1081 acgatgaacc atgcgcactc gacgcactac acgcttgatc cggaccagcg catcgctcac
     1141 gtcttgcacg gctgggcaga aggcggacgg acgccggcgg tcgatctgac ggtccgggat
     1201 atgcacgttt ccaacttgcc gacgaatatc cggacctggg gcggcgaaac ggagttgtat
     1261 gggaactcca ttttcgtgtt cgagacaggc ggattgtgca tcgcgcacct tggtcatctg
     1321 caccatctgc tgcaaccggc ggacctgaat acccttgggc acatcgatgt tgcgatggtc
     1381 gccgttgacg gcggctatac catgagccag caagacgccg ccgcggtggt ggagcagctt
     1441 cagccccgca ttgtgctgcc gatgcactat tttacacgtg gcgtgttgga gcgtttcctt
     1501 gatctggaac gcgggacata cgtcatcgat gtgcgggata gtccggagat ggagctgtcc
     1561 cgaacaagcc tgccttcagc accgacggtg atcgttctgc cgggcggtta ctgatcgcct
     1621 gggctcaacg gcaaattttc aggccgcatc gcgttctttc ggcttggcta cgtaccgcat
     1681 gtcgccggaa acgtggattt ccagcatggt tgctccggcc acgccgccca gcggcaacag
     1741 ggcgtttccg ttggtccagc ggcggatcga ggagccttgc ctttcaacgg cccaccaacc
     1801 ttccgcgagg gaaggatca
//
LOCUS       DASWQN010000153         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2392051, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000153 DASWQN010000000
VERSION     DASWQN010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392051"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VGC82_02510"
     CDS             <1..245
                     /locus_tag="VGC82_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422174.1"
                     /translation="YLDDQINRIIERRLTTPRPVIVEGISLLQLMKRIHRVPDFSIYV
                     ANPRRSGGEVMDRRLATYEAAFSPSTIANMVVKIEH"
     gene            351..767
                     /locus_tag="VGC82_02515"
     CDS             351..767
                     /locus_tag="VGC82_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422175.1"
                     /translation="MNKKFFIAWIVLFIVWMAGSFVVHGVLLHDDYARLSNLFRPEAD
                     AQRYFPWMIVAHVILSGALAWIYSRGAEATPWLPQGLRFGLAIALLTIIPTYMIYYVV
                     QPMPGMTVVKQIVFDGILMLILGVVVAFVYRTPERS"
     gene            complement(882..>2085)
                     /locus_tag="VGC82_02520"
     CDS             complement(882..>2085)
                     /locus_tag="VGC82_02520"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HEY0422176.1"
                     /translation="PFRFRMLSPTHGTFGEETPDPMVTLTTAAEFVSQQVPATMEAGG
                     SVAVSVTFKNTTSVTWTAADGYSLVPAGPSGNTLWGISTVALPAPVAPGESVTFSFTV
                     IAPSALGTYNFQWQMNLQSSGPFGQISPATAVQVIAAGPPNFEGLWWNAPAGSEAGWG
                     INLAHQADIIFATWFTYDLTGKGLWLTLTAPRTATNTYSGTLIKATGPAFDAVPFNPA
                     QVVGTSVGSGTLTFTDINNGTFAYTVNGISQTKTITRQVFGPLPTCRFGALANLTLAT
                     NFQDLWWNSPPQSEAGWGINLTHQGTRIFATWFTYDHDRTPMWLVATMLNTAPATYVG
                     TLYRTTGPPFNAVPFNAANVVGTTVGTATLTFADGATAAFSYTVNGVSQTKPITREVF
                     AAPGTVCQ"
BASE COUNT          362 a          649 c          667 g          407 t
ORIGIN      
        1 tctacctcga cgaccagatc aatcgcatca tcgagcgccg cctcacgacg ccgcgcccgg
       61 tcatcgtcga gggcatttcg ctgcttcagc tcatgaagcg cattcatcgc gttccggatt
      121 tctccatcta cgtcgccaac ccccggcggt cgggcggtga agtcatggat cggcggcttg
      181 ccacctacga ggcggcgttt tccccatcga cgatcgcgaa tatggtggtt aagatcgagc
      241 actgagggcg gcatggccgc gataggagaa acaacgtgac cgccgccgga cggcagactg
      301 cacgcccggc agccacggaa tgccgctcgt ttccatacca agggggaatc gtgaacaaaa
      361 aattcttcat cgcctggatc gtgcttttca tcgtgtggat ggccggaagt ttcgtcgtcc
      421 atggcgtgct gctgcacgat gattacgcca ggctgtccaa tttgttccgc cccgaggcgg
      481 acgcgcagcg ctacttcccg tggatgatcg tcgcccacgt cattctgtcg ggcgccctcg
      541 cctggatcta ctcgcgcggc gcggaggcca cgccctggtt gccgcagggc cttcgcttcg
      601 gcctggcgat tgcgctattg actatcatcc cgacctacat gatctattac gtcgtgcagc
      661 cgatgcccgg catgaccgtg gtcaagcaga tcgtgttcga tggcattctg atgctgatac
      721 tcggggtggt cgtggcgttc gtgtatcgca cgcccgagcg aagctgaacc gtcgcgccgg
      781 cccaatccgc cgctggtgcg agctccgcgc cccgccagtc gaggcaccgc ttcgccgacg
      841 ccgtgtcgaa gtcgactgat cacaggcagc ggcatgcgct gctattggca caccgttccc
      901 ggcgccgcga acacttcgcg ggtgatgggc ttggtctgcg aaacgccgtt gaccgtgtag
      961 gaaaacgccg ccgtggcgcc gtcggcgaag gtcagcgttg ccgtgcccac tgtcgtgccg
     1021 accacgttcg ccgcattgaa gggcaccgcg ttgaacggcg gacccgtggt ccggtacagc
     1081 gttcccacat acgtggccgg cgcggtattg agcatggtcg cgaccagcca catcggcgtg
     1141 cgatcgtgat cgtaggtgaa ccaggtggcg aatatcctcg tcccctggtg ggtcaggttg
     1201 atgccccaac cggcttcgga ctgcggcggt gagttccacc acaaatcctg aaaattggtc
     1261 gcgagcgtca ggttggcgag cgccccgaat ctgcaggttg gaagcgggcc gaatacctgg
     1321 cgggtgatgg tcttggtctg cgagatgccg ttgacggtgt aggcgaatgt accgttattg
     1381 atatcggtga aggtgagggt cccgcttccg accgaagttc cgaccacctg cgcagggttg
     1441 aacggcactg catcgaacgc cggcccggtg gccttgatca acgtcccgct gtaggtgttg
     1501 gtcgcggtcc tcggcgcagt cagggtcagc cacaggccct tgccggtcag gtcataggta
     1561 aaccaggtgg cgaagataat gtcagcctga tgcgccaggt tgatacccca gcccgcctcc
     1621 gatccggcgg gagcgttcca ccacaggcct tcgaaattcg gaggtcctgc ggcgatgacc
     1681 tgcactgcgg tcgccggcga tatctgaccg aacggaccgc tcgactgcaa attcatctgc
     1741 cactggaaat tgtaggtgcc cagagccgag ggcgcgatca ccgtgaagct gaaggtcacc
     1801 gattctccgg gtgcaaccgg cgcgggcaac gccaccgtgc tgatccccca gagggtgttg
     1861 ccggaggggc ccgcgggtac caagctgtag ccgtcagcgg cagtccaggt gacgctggtg
     1921 gtgttcttga atgtgacgct gacggccacc gaccctcccg cctccatcgt tgccgggact
     1981 tgctgcgata cgaattccgc cgccgtcgtg agcgtgacca tgggatcggg cgtctcctcg
     2041 ccgaacgttc cgtgggtcgg cgagagcatg cgaaatcgga aaggc
//
LOCUS       DASWQN010000154         3382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2260490, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000154 DASWQN010000000
VERSION     DASWQN010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2260490"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VGC82_02525"
     CDS             complement(<1..425)
                     /locus_tag="VGC82_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0422177.1"
                     /translation="MPSGNRLLGKVAIISGGASGIGAETARVFAAQGASVVLCDLQDD
                     LGQMVAKEISDSGGTADYQTLDVCSEKQWIALVAATEQKYGKVNVLGNIAGISGRPPS
                     MTVQTGTIAGTALAEQTLESWNRIMEVNATGVFLGTKSV"
     gene            complement(728..1576)
                     /locus_tag="VGC82_02530"
     CDS             complement(728..1576)
                     /locus_tag="VGC82_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0422178.1"
                     /translation="MMVERGGWRVRTLRLWLPMGLFMIFTLFPFYWMAVTSLKPNSEL
                     YNRKIMPLIVYHPTLKHYVDLLSETNFLLWTWNTFLVAVVSTAISLVFGVMLAYPLAR
                     MRFAGSALISFGVAATYLVPQPLLFIPLADIITNLGLNDTLTSVILTYPTLLIPFCAW
                     LLMGYFRSVPTELEDAARIDGATRLQTLFKVFLPLCTPGLISAGIFAFTLAQNEFLYA
                     LIFLTQTEVRTVPVGAIAELVKGDVFYWGQLMAAALLGSVPVAVIYSFFVEHYVAGLT
                     AGAVKQ"
     gene            complement(1573..2409)
                     /locus_tag="VGC82_02535"
     CDS             complement(1573..2409)
                     /locus_tag="VGC82_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY0422179.1"
                     /translation="MIIPPVVFLLLLVGYPFIYGIWLSLENRPVARAGTFVGLANFVA
                     DYHDPVFWQVTRNTFVYTIAATLLKMMGGLGLALVMNQDFRFKNMVRALMLLPFIVPT
                     VLSTIAWMWILDPAFAPVNWFLRNLGIANPGPSWLGNASLAMMSLIIVNVWRGLPFYG
                     ITLLAGLQTISPELYEAATIDGANTWDRFRFVTLPLLRPVILIVTMFSIIFTFADFQL
                     VYVLTRGGPANATHLFATYAFDVAMGGGQLGAGASIALAMLPPLALIIFALSLYMKPA
                     KS"
     gene            complement(2520..>3382)
                     /locus_tag="VGC82_02540"
     CDS             complement(2520..>3382)
                     /locus_tag="VGC82_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0422180.1"
                     /translation="PFGNVGQLMNYRMDWFNEVGVKEFPDTWDGLLEAGIKLKKAGRP
                     FGFELGHGFGDNHGWLYPLLWSYGGREVDTDGKTVAIDSAETARAVDFCRDFYQKTMF
                     EDVLGWTDVSNNKAYLSEQISCTNNAESILYIAKKDFPDIGKVTGQAQNPKGPTGQRF
                     HILAPWSHSIFTHAQDVKAAKDFLVWLMDPKQVGGWYDVAVSYYAPFLHMFDNAPLWS
                     AEPRNLPYRDSLTTSHLPGWPAPISRPQSESVAKYVVVDMFAKACAGRSTKDVIADAS
                     AQLKQIFKSA"
BASE COUNT          742 a          993 c         1011 g          636 t
ORIGIN      
        1 atcacgcttt tggtgcccag gaacacgccg gtggcgttca cctccatgat gcggttccaa
       61 ctctccaggg tttgttcggc cagggccgtg ccggcgatgg tgcccgtctg aaccgtcatg
      121 ctaggcgggc ggccggagat cccggcgata ttgcccagca cgttgacctt gccgtatttc
      181 tgttccgtgg ctgccaccag cgcgatccat tgtttttcgc tgcatacgtc gagcgtctgg
      241 taatcggcgg tacctccgct atcggatatc tcctttgcca ccatttggcc aaggtcatcc
      301 tgcaggtcgc atagaacaac gctggcacct tgtgccgcga atacccgcgc ggtttccgcg
      361 ccgatgccag aggcgccgcc ggagatgatc gctaccttgc cgagaagtcg atttccagat
      421 ggcatgtgtg tttcctcttt ctttaagacg gccggcccga cggcaccccg gtgcgatgta
      481 cgcagtcagt gcgttatcgt agacttcgaa aaaggcaccc atcggcagca ggattaccaa
      541 gccttgtgta tagcgcgtag ccggcaggcg accgatccga gccgcgattg tcgcggtctg
      601 gttgttggac atgatcggtt cttcccttgg cggtgcgact ttcggccgcc ctcgtgcttc
      661 aactatgaca cgctggcaca ggtgcgacgg ctggaccaag agcgaggggt tacttcaagc
      721 ggtggactta ctgcttcacg gcgccggctg tcagacccgc cacatagtgc tcgacgaaga
      781 aggaatagat gaccgccacc ggcaccgaac caagcagcgc ggcggccatc aactggcccc
      841 agtagaacac gtcgcccttg accaattcgg cgatggctcc gacgggcacg gtacgaacct
      901 cggtctgggt cagaaagatc agcgcgtaga ggaattcatt ctgagccaaa gtgaaggcga
      961 agatgcccgc cgatatcaag cccggtgtgc acagcggcaa gaacaccttg aacagcgtct
     1021 gcaatcgcgt ggcaccgtcg attcgagccg catcctcaag ctcggtcgga acggagcgga
     1081 agtatcccat cagcagccaa gcgcagaacg ggataagcaa ggtaggatag gtcaagatga
     1141 ctgaagttag cgtatcgttc aggccaagat tggtgatgat gtcggcgagc gggatgaaca
     1201 acagcggctg cggcaccaga tatgtcgcgg cgacgccgaa ggaaatcagt gccgatccgg
     1261 cgaaccgcat gcgcgccagc ggataggcca gcataacccc gaaaaccaac gaaatcgcgg
     1321 tggagacgac cgcgaccagg aacgtgttcc aggtccacag cagaaaattg gtctccgaca
     1381 acaggtccac gtaatgtttg agggtcggat gatagacgat cagcggcatg atctttcggt
     1441 tgtacagctc actgttcggc ttcagcgaag tcaccgccat ccagtagaac ggaaatagcg
     1501 tgaagatcat gaacaggccc atcggcagcc acagccgcag ggttctgacc cgccatccgc
     1561 cgcgttcgac catcatgatt tggcgggctt catgtaaagg gacagggcga atatgatcag
     1621 cgcaagcggc ggcagcatcg ccagtgcgat ggacgctccg gcgcccagtt gcccgccgcc
     1681 catcgcgaca tcgaaagcat aggtcgcgaa caaatgggtg gcgttggccg gaccgccgcg
     1741 ggtcagcaca tagaccaact ggaagtcggc aaaggtgaag atgatcgaga acatcgtcac
     1801 gatcagaatc accggcctga gcaagggaag cgtcacgaac cgaaagcgat cccaggtgtt
     1861 ggcgccatcg atcgtcgccg cctcatacag ttccggcgag atggtctgca gcccggccag
     1921 aagcgtgatc ccgtagaacg gcaatccgcg ccaaacattg acgatgatca gcgacatcat
     1981 cgcaagggat gcgttgccaa gccatgacgg gccgggattg gctatcccga ggttgcgcag
     2041 gaaccagttg accggcgcga acgccgggtc gaggatccac atccaggcga tcgtcgatag
     2101 cacggtcgga acgataaatg gcagcaacat caacgcgcga accatgttct tgaacctgaa
     2161 gtcctggttc atcaccaggg ccagacccag tccgcccatc attttcagca gcgtcgcggc
     2221 gatcgtgtaa acgaaggtat tccgcgtaac ctgccagaac accggatcgt ggtagtctgc
     2281 gacaaagttt gccagcccaa cgaacgttcc cgctctggcg accgggcgat tctccaggct
     2341 gagccagatg ccgtagatga acggataacc gaccaacagc agcaggaaga ccacgggagg
     2401 aatgatcatc aaccaactga ataccgcctc ccgctcggtc cagcggcgaa agccggaagc
     2461 cttgcgggct ggcggggcgc ccagtgtcgc gcttgaggca atcgttgttg ctgacacgtt
     2521 caggccgact tgaagatctg cttcaactgc gcggaggcat cggcgatgac gtctttggtg
     2581 gaccggccgg cgcaggcctt ggcgaacatg tcaaccacaa cgtatttcgc cacgctttcc
     2641 gactgtgggc ggctgatcgg ggccggccaa cccggcaaat gcgacgtcgt cagcgagtcg
     2701 cggtacggca ggttgcgcgg ttcggcggac caaagtggcg cgttgtcgaa catgtgcagg
     2761 aatggtgcgt agtagctgac ggcgacatca taccagccgc ccacctgctt cggatccatc
     2821 agccacacca ggaaatcttt cgccgccttg acatcctggg cgtgggtgaa gatcgaatgg
     2881 ctccagggtg ccaggatatg aaagcgctgc ccggtcggac ctttcggatt ctgcgcctgg
     2941 ccagtgactt tgccgatgtc ggggaaatcc ttcttggcga tgtaaagaat gctttccgca
     3001 ttgttggtgc aggaaatctg ctccgataga taggctttat tgttgctgac gtcggtccag
     3061 ccgaggacat cctcgaacat cgtcttctgg tagaagtcgc ggcagaaatc gacggcgcgc
     3121 gcggtttcgg ccgagtcgat ggcgaccgtc ttgccgtcgg tatccacctc tcgtccgcca
     3181 taagaccaca gcaatggata cagccagccg tggttgtcgc caaaaccgtg gccaagctcg
     3241 aatccaaacg gccggcccgc ttttttcagc ttgatgccgg cttccagcaa tccatcccag
     3301 gtgtccggga actccttgac gccgacctca ttgaaccagt ccatccgata gttcatcaac
     3361 tggccgacgt tgccgaacgg aa
//
LOCUS       DASWQN010000155         3496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2524259, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000155 DASWQN010000000
VERSION     DASWQN010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2524259"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VGC82_02545"
     CDS             <1..556
                     /locus_tag="VGC82_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422181.1"
                     /translation="IWNIGAIAKSGHPRALDTVRRIMLASAAIPGAFPPTMFDVTLDG
                     KPYQEMHVDGGTFAQAFLYPAAVMQSRRARVLRHQAVLPVIAYVIRNARLDPEWAAVE
                     RRTLSIAERAITTMIAASGYNDVIRIYNNTLRDGIGYNLAYIGRDFTEVLPSPFDQKY
                     MRDLFDYGYQKALHGYVWASQPPF"
     gene            complement(1025..1234)
                     /locus_tag="VGC82_02550"
     CDS             complement(1025..1234)
                     /locus_tag="VGC82_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422182.1"
                     /translation="MRKVPMARRLCEARAAALLPSARMVERRIARPLMEALAMALRYI
                     VVRYMPAPFTGHMSEHPITVQLSRA"
     gene            complement(1361..2158)
                     /locus_tag="VGC82_02555"
     CDS             complement(1361..2158)
                     /locus_tag="VGC82_02555"
                     /inference="COORDINATES: protein motif:HMM:NF021391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HEY0422183.1"
                     /translation="MQRMMTTHLTGLYVAVAMCLAIPGGSLAVAATPPAPAISEDARA
                     AVAQMGKTLHGKEFSFRARTIRPYANENGAMLHIEHDFKVTVRRPDRMLIDANGDDGP
                     VKLFYDGKTVALALDAAGSQYAALPVPDTITMMMHVVMGRFGLDFPLADFLTDAPDKA
                     FLQGVTSGQEINKVTIDGVSCVHLVFSQPPGIELELWLEADRGVPRRLIVIDNNSPGK
                     PNFSAEMSDWDFTVHPADADFAFTPPTGAKEVDFGAALSGQLTGAPQ"
     gene            complement(2674..2922)
                     /locus_tag="VGC82_02560"
     CDS             complement(2674..2922)
                     /locus_tag="VGC82_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422184.1"
                     /translation="MTTIAGIDRGEDPSMTTSTRRPMPSNARTTLTELRNARIEQAIR
                     QLPDASATRIAGLAECSPTAVVNWRKARAALARSEAAD"
     gene            complement(3078..3257)
                     /locus_tag="VGC82_02565"
     CDS             complement(3078..3257)
                     /locus_tag="VGC82_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422185.1"
                     /translation="MQTVHHRARNRDEAIRVALHKIGPISDEWVTGPNIGAFELPIEV
                     ARRLVAEVDGAYDGE"
BASE COUNT          689 a         1059 c         1081 g          667 t
ORIGIN      
        1 gatctggaac attggcgcca tcgccaaaag cggacatccg cgcgcactgg atacagtgcg
       61 ccgcattatg ctggcatcgg cggctattcc cggcgccttc ccgccgacca tgttcgacgt
      121 caccctggac ggcaagccct atcaggaaat gcacgtggac gggggcacct tcgcgcaggc
      181 gtttctctat cccgcagcgg tgatgcaaag ccggcgggcg cgggtgctgc gccatcaagc
      241 ggtgctgcca gtgatcgctt acgtcatacg caacgccagg ctggaccccg aatgggcggc
      301 ggtggagcga cgcacgctaa gcatcgccga acgggcaatc accaccatga tcgccgccag
      361 cggctacaac gatgtcatcc gcatctacaa caacacgctg cgcgacggca tcggctacaa
      421 ccttgcttat atcggcaggg attttactga ggtgctgccg tcgccgttcg accagaaata
      481 tatgcgcgac ctgttcgact acggctatca aaaggctttg catggctacg tctgggcgag
      541 ccagccgccc ttctgaatcc gtttgaccaa gttggaattc catgaggctc gacccatgtg
      601 tggcgcccta cgaataggaa ggctgcggca aaacctatcg aataaggata ttttcctatg
      661 aattagtatc tttgtaggtg cagacttgga ttcttcatgg tgctttttca tgaaaaatgt
      721 accgtgcgtc gactttgagt ggattccaga catcataggt aggagggtcc gtcaccggcc
      781 gcttcgcgcc aatccaggcc gtgagggcgg ccccgcgaag gtcggctttg ggtcgactgc
      841 agcggtttcg ggcgggtgct ggctcagccc cgcattggtc aaccagccaa aatcactgtg
      901 tggacgtact agcggccaca gtagtcccaa tacccctggt tgtgcgcggg gctcgaccag
      961 aaccagcaca aggttggtga cggtgggggt ggtggcgtcg tgagagcgat tacggtgcca
     1021 attgtcacgc ccgcgacaac tgcaccgtaa tagggtgctc ggacatatgg cctgtaaacg
     1081 gcgccggcat ataccggacc acgatatacc ggagcgccat agccagggct tccataagcg
     1141 ggcgggcgat acgccgttcc accattcggg ccgacggcag cagcgccgcc gcgcgggcct
     1201 cgcacagccg ccgcgccatt gggaccttgc gcatagccgc cgcccctggg accagtcgtt
     1261 gcggtgccgc cgcgaggacc ggtcacagtt ccccatccga atgcctgcaa tggagcggca
     1321 aaaccaaaca aaacggcgag cgagatccga atgaagcggt tcattgtgga gctcctgtaa
     1381 gctgcccgct gagagcagcg ccgaaatcca cctccttggc accagttggc ggagtgaaag
     1441 caaagtctgc atcggccgga tgcacggtga agtcccaatc ggacatttcg gcgctgaagt
     1501 tcggcttacc gggggaattg ttgtcgatga cgatgagtct tcgcggcacg ccgcggtccg
     1561 cctcaagcca aagttccagt tcgatgcccg gcggctggga aaacaccagg tgaacgcatg
     1621 acacgccgtc gatcgtcacc ttgttgatct cctggccgga cgtgacgccc tgcaagaatg
     1681 ccttgtcagg cgcgtcggtg agaaagtcgg ccaaaggaaa gtcgagcccg aagcgaccca
     1741 tcacaacgtg catcatcatc gtgatggtgt cgggaaccgg cagcgccgcg tactggctgc
     1801 cggcggcatc gagcgcaagg gcgacggtct tgccgtcgta gaacaacttg accggtccat
     1861 catcgccgtt ggcgtcaatc agcatccgat ccggccggcg aacagtgacc ttgaagtcgt
     1921 gctcgatatg cagcatcgca ccgttctcgt ttgcataggg ccggatagtc ctggcccgaa
     1981 acgagaattc cttgccgtgc aatgtcttgc ccatctgcgc gacagcggcg cgagcgtctt
     2041 cactgatcgc tggggccggc ggagtggcgg caacagcaag tgagccgccc ggtatcgcca
     2101 ggcacatggc aacggcaacg tacaggcctg taagatgcgt cgtcatcatg cgttgcatgg
     2161 ttcttctccg acggttatcc ggtatggagt tcagtttgat accttggcat tgccccgccc
     2221 taactccatt gccggagcgt gaaccgcgcc gcattggcgc ggaatgcgag tccgaaggac
     2281 caagaagcac tccggctttc aacttactat gcattctcgg cggaaatgcc aaacgccccc
     2341 tttgtcgccg ggcgattcac ccgcagacag tcggaaaccg cataatctcg gtgtatgaca
     2401 agcccgctcc gttaccgtgc aacgaacaga cgggcccgcg gtcacgccgg catatttttg
     2461 tcatatcacg taattctcac gacagcccgt cgcgcggcgt tatttcgtga ggtgacaaaa
     2521 tatacgagcg agcggctgac gatggtcgaa acgcaggtcg gcggcctggt gggtgcggaa
     2581 cagtcgcatt atgccagcgc gatacagcgc ttcgacagca tggagcggcc gatggccagg
     2641 atcgagcgac ggctggacct gaccgacacg ccctcagtca gcagcctcgg accgggccaa
     2701 cgccgcccga gccttgcgcc agtttacgac cgcagtcggg ctgcactccg ccaggccggc
     2761 gatccgcgtg gcggatgcgt cagggagttg ccggatggcc tgctcgatcc tggcgttgcg
     2821 caattctgtg agcgtcgtgc gcgcattgct gggcatgggc ctgcgggtcg aggtcgtcat
     2881 cgacgggtct tcgcctcgat caatcccagc gatagtcgtc atgtcgcttc agccccagat
     2941 gctggaggat tggcagccgt ggcttttgcc gggcgtcgca tacatcagtg accagggcag
     3001 gatcgaggcc acaagcggtt gcccaggctt ggatactgcc ggccttgtgg cactcgcggc
     3061 gaagcatgtg tcgcacttca ctcgccgtca taggcaccgt ccacttccgc gaccaaccgc
     3121 cgcgcgactt caattggcag ttcgaatgcg ccgatgttcg gtccggtaac ccactcatcg
     3181 ctgatcgggc caatcttgtg cagagcaacg cgtatcgcct catcgcggtt gcgagcgcga
     3241 tgatggacgg tctgcacacg tgattctcca ccgtagaccg ccgcccggga cgcgggaacg
     3301 gtcacgatga aaagcggcat caccacgtta gcggcgaccc agatgaaatc gctcgcggaa
     3361 ctgcgcggcg gttgtgccgt aatccgggcg agatggcggc tcgtatggca cgtagggcga
     3421 cctcaatcgc ctcggcggtg gcgggaggtg cagcttaggg ccggccaagt cgtctggttt
     3481 tccgccgttt tgcacg
//
LOCUS       DASWQN010000156         5975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_412993, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000156 DASWQN010000000
VERSION     DASWQN010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412993"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..956)
                     /locus_tag="VGC82_02570"
     CDS             complement(141..956)
                     /locus_tag="VGC82_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEY0422186.1"
                     /translation="MRILFLGDVLGRSGREAVAARLPGLRHDLRIDLTVVNAENASHG
                     FGLAPDMARALFAAGADVITLGNHAWDRKEIIPYIAETPRLLRPLNYPPGTPGAGSVL
                     VTLQDGRKALVLQAMGRLYMDPLDCPFRGTAEILSKYRLGASVQAIVADIHAEASSEK
                     MAYGHIFDGQMSFVVGTHTHCPTADAQILPGGTAFQSDAGMCGDYDSVIGMAKDGATL
                     RFWRKMPGEKLGPAEGEATVCGVFVETDDATGLSRRIEPVRVGGRLAPAMPSG"
     gene            complement(958..1518)
                     /locus_tag="VGC82_02575"
     CDS             complement(958..1518)
                     /locus_tag="VGC82_02575"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY0422187.1"
                     /translation="MTSALDAAKQAAREAALARRHGLDAKSAGHALAAHVLRDCPPPS
                     DAVVSGFWPLGDEIDIRPLLLALHGRGNRVVLPVTPRRGERLTFRGWQPGDAMVPERF
                     GTMRPIGDILEPDMLLIPLLAFDLAGGRLGYGGGFYDRTLAGLPNRFRLGCAFAAQQV
                     DAVPIGPYDARLDAVATENGIIRCGG"
     gene            complement(1534..1690)
                     /gene="ssrS"
                     /locus_tag="VGC82_02580"
     ncRNA           complement(1534..1690)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VGC82_02580"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1825..2685
                     /locus_tag="VGC82_02585"
     CDS             1825..2685
                     /locus_tag="VGC82_02585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY0422188.1"
                     /translation="MRLNAFFPTRDIGSDPAKIRDWAQAAEDLGYADIEVPDHVFGAT
                     ARGDWKPIYTEADPFHETFTTLAFLAAVTKTIKLTSGVLILPQRQTGVVAKQAAEVDI
                     LSGGRLRLGIGVGWNHVEYQSLGAEWHTRGVRQAEQIEVMRRLWTEDLVTFNGRFHDL
                     QSVNIVPPPIQRPIPIWFGGSSDAVTKRAARLGDGWMPIMAPDEQGEAKLEEVRGHLK
                     AFGRDPATFGIEGWVRMTEPNPDGWAEAVAGWRRLGADMVMHYPMYRLPTFDQQIETL
                     RRFKDAAGAA"
     gene            2725..3870
                     /locus_tag="VGC82_02590"
     CDS             2725..3870
                     /locus_tag="VGC82_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0422189.1"
                     /translation="MSQQKQWIGTWTAAPAPAEGVAFSNITLRMNARVSLGGDVIRVR
                     LSNAHGAKPLTIGAAHVGLRASGPAIQPDTNRRLSFGGDGAATIAAGSLLISDPVELE
                     VPALGDVVVTVHLPDDLPASFGITGRYARQTNYISPLGNFAAEAVMPVGRITDDWYFV
                     SGIDVLAEPTTGGIVALGDSLTDGNISTHDAFASWPSQLARRLASRAGGRTLGVMNQG
                     LGGNRILHDIRGDSGLRRFDRDVLAAPGVTHVIVVLGTNDLRNRWRKPEEEATAPSMI
                     AGLKQLAVRAHARGIKIFGGTIPPFENETFLPGAWSPAREAVRQAVNAWMREANAFDS
                     VIDFDAGLRDPEHPTSMLPRYDCGDHLHPSDAGYHRMGDIIDLSLFG"
     gene            complement(3948..4274)
                     /locus_tag="VGC82_02595"
     CDS             complement(3948..4274)
                     /locus_tag="VGC82_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY0422190.1"
                     /translation="MPQVTLRINGYSYTIGCKEGEESHLEAMGAEVTRRIEGVRLAAG
                     PSGEARMLVMAALLMADDIFELRRKLEETTPAEAQAKPDPKLSRKLNRMAKRAEEIAE
                     GLEGPV"
     gene            complement(4294..4509)
                     /locus_tag="VGC82_02600"
     CDS             complement(4294..4509)
                     /locus_tag="VGC82_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422191.1"
                     /translation="MAEANMTDEPEDPEAAADRLEAALERIARLAAIQPAQTNAPVPD
                     SELSIPEIAERLDALISRLRAALGKTT"
     gene            4607..5038
                     /locus_tag="VGC82_02605"
     CDS             4607..5038
                     /locus_tag="VGC82_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422192.1"
                     /translation="MIVHPAVIVHGLDDARAALAPGLPVTLLSAPAAALFAGCLWWRQ
                     MVAAARLDYKDTEAIDVLDCADGSGMAMAALRSGVCRLVVWPNAPGWDRLAAIAGRQG
                     GFIVTRAPSALDLAERNAIRRLHLWLLDSGRAGGDSSASVR"
     gene            5204..5851
                     /gene="thiE"
                     /locus_tag="VGC82_02610"
     CDS             5204..5851
                     /gene="thiE"
                     /locus_tag="VGC82_02610"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY0422193.1"
                     /translation="MDSGNDQGCRVYLITPPKLDPRLFADLLAAALDAGDVAAVQLRL
                     KDVDDDAWRRAIDALRPVCQSRDVAFLLNDRADLVRPTGCDGAHVGQDDMPAKDARRL
                     LGADLMLGVTCKGSRDLAMQAGEDGADYVAFGAFYPSTTKEVTRLLDPEILRWWSEMM
                     ELPSCAIGGITPENCGPLVQAGTDFLAVVGCVWNHPDGPAAGVKALNAAIKDAGR"
BASE COUNT         1049 a         1882 c         1956 g         1088 t
ORIGIN      
        1 gcttgataga gtcgttgccg agcagatcgg acaggatctg cgtcaaggtg gcaagccgtt
       61 cctgctctgt cataaccaat cagatcctgc atcttcatgc tcggatcgtg atgccacacg
      121 aagccgtgag atgccaagac ttaaccgctt ggcatcgccg gcgcgaggcg cccaccgacg
      181 cgaacaggct ctatccggcg agacaacccg gtggcgtcgt cggtttcgac gaacacgccg
      241 cagacggttg cttcgccttc cgccgggccg agtttctccc ccggcatctt gcgccagaag
      301 cgcaaggtgg caccgtcctt tgccatgcca atgacgctgt cgtagtcgcc gcacatgccg
      361 gcgtcggact ggaacgccgt gccgccggga aggatttgcg cgtccgcggt cgggcagtgg
      421 gtgtgggtgc cgacgacaaa tgacatctga ccgtcgaaga tatgcccgta cgccatcttc
      481 tcgctgctgg cttcggcatg gatatccgcc acgatcgcct gcaccgaggc acccaggcgg
      541 tatttcgaca ggatctcggc ggtgccgcgg aacgggcagt ccagcgggtc catgtacaag
      601 cgtcccatgg cctgcagcac gagtgccttg cggccgtcct ggagcgtcac cagaaccgac
      661 cccgccccgg gcgtgccggg cggataattc agcggccgca gcaaccgagg cgtttcggcg
      721 atgtagggga tgatctcctt gcggtcccag gcatggttgc ccagcgttat gacgtcggcc
      781 ccggcggcaa acagcgctcg ggccatgtca ggcgccaggc cgaaaccgtg cgacgcgttc
      841 tcggcattga ccaccgtcag atcgatccgc aagtcatggc gcagaccggg caatcgggca
      901 gcgacggcct cccgtccgct gcgccccaga acgtctccca aaaacagtat tctcaattca
      961 gcctccgcag cggatgatgc cattttccgt cgcgacggca tccagccttg cgtcgtaagg
     1021 gccaatgggc acggcgtcca cctgctgcgc ggcgaatgcg cagcccaggc ggaaccggtt
     1081 cggcaatccg gccaaagtcc ggtcgtagaa gccgccacca taacccagtc gcccgcccgc
     1141 gagatcgaag gccagaagcg ggatcagcag catgtctggc tccaggatgt cgccgatcgg
     1201 gcgcatcgtg ccgaaccgct ccggcaccat cgcatcaccc ggctgccatc cgcgaaaggt
     1261 gagccgctct ccgcggcgtg gcgtcaccgg caggacgacc cggttccccc ggccatgcag
     1321 cgccagcagc agcggcctga tgtcgatctc atcgcccagc ggccagaagc cggaaacaac
     1381 agcatcggac ggtggcggac agtcacgcag cacatgggca gcgagggcgt gcccggctga
     1441 cttggcatcc aaaccatgac gacgcgccaa tgccgcctcg cgcgctgcct gcttggctgc
     1501 gtcgagcgca ctcgtcatgt gggaaaaaat ctgtgcggag ccaccatggc cgttggcgta
     1561 tcatcctccc agagcctgct tgtgcaggtg ggcaccggat accgagacca agatcccgac
     1621 agagccagct cccgtgggag atgcttactg gccccgggga tgagttacct gacgcaccgg
     1681 gtagccccgc acgatctact tagggatcag caatcgaata agcaaggcat tgcgccataa
     1741 cgcttcggct gcttttggtt gagcgggttt ggcaggaacg cgcttcggga ccagactgct
     1801 ttggaacctg cggtggagtg tgccatgcgc ctcaatgcct tcttcccgac acgcgatatc
     1861 ggcagcgatc cggcaaagat cagggactgg gcgcaggcag ccgaggatct cggctatgcc
     1921 gatatcgaag taccggatca cgttttcggc gccaccgcgc ggggcgactg gaagccgatc
     1981 tataccgagg ctgatccgtt ccacgagacg ttcacgacgt tggccttcct cgccgctgtc
     2041 accaagacga tcaagctgac gagcggggtg ctgatcctgc cccaacgcca gacgggcgtg
     2101 gtcgccaaac aagcggcgga agtggacatt ctcagtggcg ggcggttgcg gttgggcatc
     2161 ggcgtcggct ggaaccatgt cgaataccaa agtctcggcg cggagtggca cacaagaggc
     2221 gtgcgccaag ccgaacagat cgaggtcatg cgccggctgt ggaccgagga tctggtgacc
     2281 ttcaacggtc ggttccatga tctgcaatcc gtcaacatcg tcccgccgcc gatccagcgc
     2341 cccattccaa tctggttcgg cgggtcttcg gacgccgtga ccaagcgtgc cgcacggctc
     2401 ggggacggtt ggatgcccat catggcgccc gacgaacagg gcgaagcgaa gctggaagaa
     2461 gtgcgcggcc acctgaaggc gttcgggcgc gatcctgcca ctttcggaat agagggctgg
     2521 gtgcgcatga cagagccgaa cccggacggt tgggccgaag ccgtggctgg ttggcggcgc
     2581 ctcggggcag acatggtgat gcactatccg atgtatcgcc tgccgacctt cgaccagcag
     2641 atcgaaaccc ttcggcgctt caaggacgca gccggagccg cctgagccaa gtccgcgtcg
     2701 cgttgaatga tcagggagga aacgatgtcc caacaaaaac aatggatcgg cacctggacg
     2761 gcggcgccgg ccccagccga gggcgtggcc tttagcaaca tcaccttgcg catgaatgcg
     2821 cgcgtcagcc tgggcgggga cgtgatccgg gtgcggctgt ccaatgccca cggcgccaaa
     2881 ccgctgacca tcggcgctgc ccacgttggg cttcgcgcat cgggacccgc catccaaccc
     2941 gacaccaatc gccgcctgag cttcggcggc gacggcgcgg cgacgatcgc cgccggatcg
     3001 ctgctgatca gcgaccccgt cgaactcgaa gtgccggcat taggcgacgt ggtcgtgacg
     3061 gttcacctgc ccgacgatct tcccgccagt tttgggataa ccgggcggta cgcccgccaa
     3121 accaactaca tctcgccgct gggcaacttc gccgcggagg cggtgatgcc ggtcggccgc
     3181 atcacggacg actggtattt cgtcagcggc atcgacgtgc tggcagaacc gacaaccggg
     3241 ggcatcgtcg ccctcggcga ctcgttgacc gacggcaaca tctcgaccca tgacgccttc
     3301 gccagttggc ccagccagct tgcgcgccgg ttggcatcca gggccggcgg ccgaacgctt
     3361 ggggtgatga accaggggct gggtggtaac cggatcctgc atgatatccg cggcgacagc
     3421 gggctgcgcc gcttcgatcg ggatgtgctc gccgcgcctg gcgttacaca tgtcatcgtc
     3481 gtgttgggca ccaatgacct gcgcaaccgc tggcgtaagc cggaagaaga ggcgacggcg
     3541 ccgtccatga tcgccggtct gaagcaactg gctgttcgtg cccatgcgcg cgggatcaag
     3601 attttcggcg gcacaatccc gccgttcgag aacgagacct tcctgccagg cgcctggagc
     3661 ccagcgcggg aggccgttcg gcaggcggtc aacgcctgga tgcgcgaggc aaatgctttc
     3721 gattcggtga tcgatttcga cgctggactg cgcgacccgg agcacccgac cagcatgttg
     3781 ccgcgttacg actgtggcga ccatctgcat ccgagcgatg ccgggtatca ccgaatgggc
     3841 gatatcatcg acctgtcgtt gtttggctag tcgcacggtc gcgctagtcg cacggtcgcg
     3901 ccgggcggaa cgtccacagc gcgtgaatca ggcgggggaa atatagccta taccggtcct
     3961 tcaagacctt cggcgatctc ttccgcgcgc tttgccattc ggttgagctt gcggctcaat
     4021 ttcggatcgg gcttcgcctg ggcctcggcg ggcgtggttt cctcgagttt ccggcggagc
     4081 tcgaaaatgt cgtccgccat caacagcgcc gccatcacca gcatccgcgc ctcgccgctt
     4141 ggtcccgccg cgaggcgaac gccctcgatt cgcctggtga cttcggcacc catggcttcg
     4201 agatggcttt cctcgccctc tttgcagcca atcgtgtacg aataaccatt gatgcgaaga
     4261 gtgacctgcg gcatcgcgtc atcctcctgt aattcaggtt gttttgccga gcgcggctcg
     4321 caaacggctg atcagcgcgt cgaggcgttc cgcgatctcc ggtatcgaca gctcggaatc
     4381 cggcactggc gcattggttt gcgcgggttg gatggcggcc agacgcgcga ttcgctccag
     4441 agccgcttcc aaccggtctg ccgccgcctc cggatcctcg ggctcgtccg tcatgttcgc
     4501 ctctgccatc tcccctctgc catctccctt ctgccgtctc ccttcggcca tcacccgatg
     4561 ctgatgccgc ttgaactcgg cgcggtcaac cggttgaacg tcgcttatga ttgttcatcc
     4621 ggcggtaatc gtccatgggc ttgacgatgc gagggcagcg ctggctcctg gtttgccggt
     4681 gacgctgctg tcggcgccgg ccgcggcgct gttcgcgggt tgcctttggt ggcggcagat
     4741 ggtcgccgcg gctcggttgg actacaagga caccgaggcg atcgacgtcc tggactgcgc
     4801 cgatggatcg ggaatggcga tggcggcgtt gcgcagcggc gtttgccggt tggtcgtgtg
     4861 gccaaatgcg cccggctggg atcgcctcgc cgcgatcgcc ggacgccagg gtggcttcat
     4921 cgtgacgcgg gcaccatccg cgttggacct tgccgaacgg aacgcgatcc ggcgactgca
     4981 cctctggctg ctggattccg gccgggccgg tggtgacagc agtgcgtctg tgcgttagtc
     5041 acgccaatac ggtcggaagc gtctcctctg gaaaggacga attcctatgt gcatcacccg
     5101 gaaggtaaag acggtcgtcc cttggtatag atacggtaac tccggtacga ggtcccgcct
     5161 tacccgcatc ctatctagtg gcagggtctg aatcttggca gcgatggaca gtgggaatga
     5221 ccaaggctgc cgcgtctatt tgatcacgcc gccgaagctg gatccccggc tatttgctga
     5281 cctattggca gcagccctgg acgcgggcga tgtggcggcg gtgcagcttc ggttgaaaga
     5341 cgtggacgac gacgcctggc ggcgggcgat cgatgcgtta cggccggtct gccagtcccg
     5401 cgacgtggca ttcctgctca acgaccgtgc cgatctggtt cgcccgacag ggtgtgacgg
     5461 tgcccatgtc ggtcaggacg atatgccggc caaggatgcg aggcggctgt tgggcgcgga
     5521 tctgatgctt ggcgtaacct gcaaaggcag ccgggatctg gcgatgcaag ccggagagga
     5581 tggcgcggat tacgtggcat ttggggcgtt ctacccgtcc accacgaagg aggtgacgcg
     5641 gttgctggat cccgagatcc tgcgatggtg gtcggaaatg atggaactgc cgagttgcgc
     5701 gatcggcggc ataacgccgg agaattgtgg tccactggtt caggcgggga cggatttcct
     5761 ggcggtcgtc ggatgtgtct ggaatcatcc ggatggaccg gcggcggggg tcaaggcgtt
     5821 gaacgcggcc atcaaagacg cgggtcgcta gcgcgggtgg ggccacaccg acttgtccag
     5881 ctagctgcag gactgcccga ggcgactaaa tggaagacgc ccagcatgtc catcgtgcgg
     5941 gctggtttcc tcgggtgatg gcgggggcgc atctg
//
LOCUS       DASWQN010000157         4097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_809403, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000157 DASWQN010000000
VERSION     DASWQN010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809403"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1143)
                     /gene="modC"
                     /locus_tag="VGC82_02615"
     CDS             complement(64..1143)
                     /gene="modC"
                     /locus_tag="VGC82_02615"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEY0422194.1"
                     /translation="MSFSLALRHRFPSFELDIDFTVQSPGVTVLFGPSGSGKSTIINA
                     AAGLLRPDTCRIELDGRVLADRRAGIWLQPEQRRAGLVFQDSRLFPHMSVATNLRFGM
                     RRAPPGSVDFDETTELLGIAGLLGRRPHTLSGGERQRVAIGRALLSQPHLLLMDEPLA
                     SLDATRKAEILPYLARLKTALNLPVLYVTHALDEAVQLADSLVLVEAGRVVASGTVSE
                     VAARADLPLARRDDAGALLICRVQENDVARALTRLEGGGAMFWVPLLDLPVGAGCRIR
                     IPAREVILAGRPPDAISLHNVVPGTVRRIAEDVVRRSVVVEIGLPEGGLLSRVTPDAV
                     VRLGLTPGSPVLALIKSTSIEVLSG"
     gene            complement(1140..1850)
                     /gene="modB"
                     /locus_tag="VGC82_02620"
     CDS             complement(1140..1850)
                     /gene="modB"
                     /locus_tag="VGC82_02620"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEY0422195.1"
                     /translation="MVYTAFMPMLSPDEWQAVRLTLTVAARAVGFGLPVAVLMAWVLA
                     RCRFPGRTILNALVHLPMVLPPVVTGWMLLIIFGLRGPVGSLLNDWFGVRLVFTSTGA
                     TLACAVMTFPIMVRSVRLSLDSIDGGLEQAARTLGAGWLDRLVNITLPLASPGILVGA
                     IVGYATCLGEFGAVITFAANIPGQTQTLPLAIYTALQVPGGEAKAATLSMVSLTLALL
                     GLLLSEWIGKRVNSALGR"
     gene            1978..2385
                     /gene="msrB"
                     /locus_tag="VGC82_02625"
     CDS             1978..2385
                     /gene="msrB"
                     /locus_tag="VGC82_02625"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY0422196.1"
                     /translation="MKVKTYPVERTEAEWRQMLTPEQFYVMRAHGTEAPGSCALLAEK
                     RPGHFTCAGCGQKLFETKLKFESGTGWPSFNDPIEGSVETSVDSSHGMIRDEVHCATC
                     GSHLGHVFPDGPPPTHLRYCINGIAMNFTADKG"
     gene            2388..3254
                     /locus_tag="VGC82_02630"
     CDS             2388..3254
                     /locus_tag="VGC82_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016738520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0422197.1"
                     /translation="MTQHPALAAGRVAVITGAASGIGLAAAVRFAGMGLKVCLADLSP
                     EALERAAETVAAASPSGTGAVLTVPTDVSQLASVQGLRDAVYAKFGEVGILMNNAGTS
                     PGGGPWDNIDRWRRVLEVNLWGVINGVQTFTPAMLAQRNPCAVVNTGSKQGITCPPGD
                     TAYNVSKAGVKVMTEALAHSLRNEEGARITAHLLVPGSTFTGMTRGGRTEKPPGAWTP
                     DQVVDMLIAGMNQGDFYIICPDNDVTRTIDNRRILWAAQDITRNRPPLSRWHPDYKAS
                     FEAFLNAAEEPE"
     gene            complement(3258..>4097)
                     /locus_tag="VGC82_02635"
     CDS             complement(3258..>4097)
                     /locus_tag="VGC82_02635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0422198.1"
                     /translation="GFGSIIGGQLFAIVGPYGWRYVMFAGVLPAIILVLLRRGLEEPE
                     HFQAVDARRNSIRAQRERSLEDQEYMRFVPAQLFSPALRYSTFIGVLFCVGTLLSIWT
                     SAIWLPTIQGQLLALQGITGPAAAPFIGHGMTLWGLGGIVGYATFGFIADVIGRRATI
                     IFYNVGAIVAGLYMYLWLDTYDLYPYVLPVFGYFVFGVFSGHAVYLPELFPTHVRATA
                     VSFCNGSGRVITSFGPLVAGLLAGSLNGAFNQATAVMTCFAGLSIVAALLGRETRDDE
                     LPK"
BASE COUNT          842 a         1399 c         1243 g          613 t
ORIGIN      
        1 gccgatctcg aggcttgatc cgcggtgacc ttcagtcgct caggatctcg cccgcggcga
       61 ccttcagccg cttaggactt caatcgacgt agacttgatc aacgccagca cgggggatcc
      121 tggtgtcagc cccagccgta ccaccgcatc cggcgtaacg cgcgacaaca ggccgccctc
      181 gggcaacccg atctccacca caaccgaacg ccggaccaca tcctccgcga ttctgcgcac
      241 tgtccccggc acgacgttgt gcaaactgat cgcgtcgggc ggccgtcctg ccagtatgac
      301 ctctctggcg gggatgcgga tgcggcagcc agccccgacc gggaggtcga gcaacggtac
      361 ccaaaacatc gcgccgccac cctccaggcg ggtcaacgcc cgcgcgacat cgttctcctg
      421 cacgcgacag atcaacaacg ccccggcgtc gtcgcgccgc gccagtggca gatcggcccg
      481 agcggccact tcggaaacgg tgccgctggc cacgacgcgg cccgcttcga caagtaccag
      541 cgaatccgcc agttgaacgg cctcgtccag cgcatgcgtg acataaagca ccggtaggtt
      601 cagtgccgtc ttcaagcgag ccagatacgg caggatctcg gccttccgtg tggcatccag
      661 gctggccagg ggctcatcca tcagcagcaa atgcggctgc gagagcaaag cccgcccgat
      721 cgcgaccctt tggcgttcgc cccccgacag tgtgtggggc cgccggccca gcagccctgc
      781 gatgcccaaa agctcggtgg tttcatcaaa atcaacgctc cccggcgggg ctcttcgcat
      841 gccgaaacgg aggttcgtgg cgaccgacat atgcgggaac agcctggaat cctgaaacac
      901 cagccccgcc cgccgttgtt ccggctgcaa ccatatcccg gcgcgccggt cggctaatac
      961 cctgccgtcg agctcgatgc ggcaggtgtc gggacgcagc aaacccgcgg cggcattaat
     1021 gatggtcgat ttgccggatc ccgacgggcc gaacaagacc gtgaccccag gagactgaac
     1081 cgtgaaatcg atgtccagct cgaacgatgg aaaccggtgc cgcaacgcga gggaaaagct
     1141 catcgcccca acgcgctgtt cactcgcttg ccgatccatt ccgacagcaa cagccccaag
     1201 agcgccagcg taagcgaaac catcgacaac gtcgctgctt tcgcttcgcc acccggaacc
     1261 tgcagtgcgg tgtaaatcgc cagcggcagg gtttgcgtct ggccagggat gttggcggcg
     1321 aaggtgatca ccgccccgaa ctcgccgagg caggtggcgt agccgacgat tgcccctacc
     1381 aaaatgcccg gcgaggccaa cggcaacgtg atgttgacca accggtccag ccatccggcg
     1441 cccagcgtgc gagccgcctg ctccaatccg ccatcgatcg agtccagcga cagcctgacc
     1501 gaacgcacca tgatcgggaa cgtcatcacc gcacaggcaa gagtcgcgcc ggtggaggtg
     1561 aacaccagcc ggacaccgaa ccagtcgttc agcaggctcc cgaccgggcc gcggagtccg
     1621 aagatgatca gcagcatcca gccggtgacg accggcggca ggaccatcgg cagatggacc
     1681 agtgcattca ggatcgttct gccgggaaag cggcagcggg ccagcaccca ggccatcagg
     1741 acagcaaccg gcaaaccaaa accaaccgcg cgcgccgcca cggttagcgt cagccggacc
     1801 gcctgccatt cgtccggcga tagcatcggc atgaaagcgg tataaaccat gatcgcgccg
     1861 ggcggaacgc cggcggcgca tgaaacgctc tgacggcgaa gaaacagcgg ccaagccgtc
     1921 acgtattgtc ctatggctgc ctggcgccaa atacaggtct gcgagaagga gcctgtcatg
     1981 aaggtgaaaa cctacccagt ggaacggacc gaagccgaat ggcgtcagat gctgacgcca
     2041 gagcagttct acgtgatgcg cgcgcatggc accgaagcgc ccggtagctg cgcattgctt
     2101 gccgagaagc gacccggcca cttcacttgc gcgggatgcg gccagaagct gttcgagaca
     2161 aagttgaagt tcgagagcgg caccggctgg cccagcttca acgacccgat cgagggctcc
     2221 gtggaaacgt cggtggacag cagccacggg atgatccgcg acgaggtgca ttgcgcgacc
     2281 tgcggcagcc atcttgggca tgtgttcccg gacggtccgc cgccgaccca tctgcggtat
     2341 tgcatcaacg gcatcgcgat gaatttcacc gccgataaag gatagccatg acccagcacc
     2401 ccgccctggc cgccggccgt gtagccgtca tcaccggtgc cgccagcggc atcggtctcg
     2461 ccgccgccgt tcgcttcgcc ggcatgggcc tgaaggtttg tctcgccgac ctgtcgcccg
     2521 aggcgctgga acgtgccgcc gaaaccgttg ccgccgcgtc gccgtccggg actggcgcgg
     2581 tgctgacggt gcctaccgat gtcagtcaac tggcctccgt gcaggggctt cgcgatgccg
     2641 tttatgcgaa attcggcgag gtcggcattc tgatgaacaa tgcgggcact tcgccgggcg
     2701 gtggcccatg ggacaacatc gaccgctggc ggcgcgtgct cgaggtcaat ctatggggcg
     2761 tcatcaacgg cgtgcagact ttcacacccg cgatgctggc gcagcggaac ccgtgcgccg
     2821 tcgtcaacac ggggtcgaaa caaggcatta cctgcccacc cggcgacacc gcctataacg
     2881 tcagcaaggc cggcgtgaag gtgatgacgg aggccctcgc ccatagcctg cgcaacgaag
     2941 aaggtgcccg catcacggcg catctgctgg ttccgggctc gaccttcacc ggcatgacac
     3001 ggggcggacg cactgaaaag ccgcctggtg cctggacgcc ggaccaggtg gtggacatgc
     3061 tgatcgccgg catgaaccag ggtgactttt atatcatctg cccagacaac gacgtgaccc
     3121 gaacgatcga caaccgtcgg attctctggg ccgcgcagga catcacccgg aaccggccgc
     3181 cgctgtcgcg ttggcacccg gactacaaag ccagcttcga ggccttcctg aatgcggcgg
     3241 aggagccgga atagggttta tttcggcagt tcgtcgtccc tcgtttcccg ccccagcagc
     3301 gccgcgacga tgcttaatcc ggcgaagcag gtcatcaccg ccgtcgcctg gttgaacgcg
     3361 ccattcagcg atcccgccag caggccggcg accagcggtc cgaaactggt gatcacccga
     3421 ccggagccat tgcagaacga taccgccgtc gcccgcacgt gcgtcgggaa caactccggc
     3481 aggtacaccg catggccgga gaagaccccg aacacgaagt acccgaacac cggcagcacg
     3541 tacggataca ggtcatacgt gtccagccac agatacatgt acagcccggc cacgatggcg
     3601 ccgacattgt agaagatgat cgtcgcgcgg cggccaatca cgtccgcgat aaagccgaac
     3661 gtcgcgtagc cgacgatgcc gcccagtccc cacaacgtca tgccgtgccc gataaatggt
     3721 gccgccgccg gcccggtaat gccttgaagc gccagaagct gcccctggat cgtcggcagc
     3781 cagatcgcgc tcgtccagat cgacagcagc gtgccgacac agaacagcac gccgatgaac
     3841 gtgctgtagc gcaaggccgg cgaaaacaac tgcgccggca caaaccgcat gtactcctgg
     3901 tcctccagcg agcgttcccg ctgcgcccgg atcgaattgc gtcgggcatc caccgcctgg
     3961 aaatgctccg gctcttccag cccacggcgc agcagcacca ggattatcgc cggcagtacc
     4021 cccgcgaaca tcacgtaacg ccagccatac ggtccgacga tcgcaaagag ctggccgccg
     4081 atgatgctgc cgaagcc
//
LOCUS       DASWQN010000158         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_149369, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000158 DASWQN010000000
VERSION     DASWQN010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149369"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VGC82_02640"
     CDS             <1..849
                     /locus_tag="VGC82_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0422199.1"
                     /translation="AAAGLHRGWVQSTGWRPQGREHDGLDVNMTDEILYNRRDGIGTI
                     TFNRPQARNALTFPMYERLAEIAADPGGVRAVIITGSGEKAFAAGTDINQFRDFSSAD
                     DALAYEARIDRVLGAIERCPVPTIAAIAGACTGGGAAIAAACDLRIAAANARFGFPIA
                     RTLGNCLSMSNLVRLSALVGSARVIEMIFTARLYSAAEALSAGLVTEVLPDQQGLETR
                     ALELGNLLAGHAPLTMRATKEALRRMRDHLPPDEDLIRLCYTSQDFKEGMDAFLTKRP
                     PAWRGA"
     gene            918..1556
                     /locus_tag="VGC82_02645"
     CDS             918..1556
                     /locus_tag="VGC82_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0422200.1"
                     /translation="MKYLTDFADQAVVLPLVLAVAVALAVQGWRRGAVVWLVVVAATL
                     LATLAFKLMFLSCSPLFGPMDVHSPSGHVAAATVVCGGLAAMLTRHRATILPAAFLAA
                     IVIGISRIVLGAHSLPEVVVGALIGLLGAAALLRFAGPPPRLRIAPLIVVIVIMAGIF
                     HGMHLPAEAAIRHSAFRAAQFIPACRGAPVFQHPRDETQLPPQPVPIAPRLA"
     gene            1617..>1912
                     /locus_tag="VGC82_02650"
     CDS             1617..>1912
                     /locus_tag="VGC82_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HEY0422201.1"
                     /translation="MPGTTRRGLGLGLAATLASRSARADLAALEAAARREGSVTWYVA
                     QMSGEAAEAMARRFTQRYPGVSVSAIRTTGQVAYERLQQELKNNTPTCDVFSST"
BASE COUNT          295 a          634 c          612 g          371 t
ORIGIN      
        1 gcagccgccg gcttgcatcg cggctgggtc caatctacgg gatggcgtcc ccagggacgc
       61 gagcatgacg gactggacgt aaacatgacc gacgaaattc tctacaaccg tcgcgatggc
      121 atcggaacca tcaccttcaa ccggccgcag gcgcgcaacg cgctgacgtt tccgatgtat
      181 gagcggttgg cggaaatcgc cgccgatccg ggcggcgtcc gcgccgtcat catcaccgga
      241 agcggtgaga aggcatttgc cgccggcacc gatatcaatc agttcaggga tttctccagc
      301 gccgacgacg ccctggcgta tgaagcccgc atcgaccgcg ttcttggtgc catcgagcgt
      361 tgtccggttc ccaccatcgc cgcgatcgcc ggcgcctgta ccggcggagg tgcggcgatc
      421 gctgccgctt gcgacctgcg gattgccgcc gccaatgctc gcttcggctt tccgatcgcc
      481 cgcaccttgg gtaactgcct ctcgatgtcg aaccttgtcc ggctatccgc gctggtcggg
      541 tcagcccggg tcatcgagat gatcttcacc gcacgcctgt actccgcggc cgaggcgctg
      601 tcggccggcc tggtgaccga agtgcttccc gaccagcaag gactggaaac gcgtgcgctg
      661 gaactcggca atctgcttgc cggacacgcc ccactgacga tgcgggccac caaggaagcg
      721 ttgcgtcgca tgcgggacca tttgccgccg gacgaagacc tgatccgcct ttgctacacc
      781 agccaggact tcaaggaagg aatggacgca ttcctcacca aacgcccgcc ggcatggcgc
      841 ggcgcctgat gaggtttttt ccgccatgac gccgatctgt accctttgtt aatattcggt
      901 ggtctataca ggtcggcgtg aagtacctga ccgattttgc cgatcaggcg gtcgtcctgc
      961 cgctggttct tgccgtcgcc gttgccctgg ccgtccaggg ctggcggcgc ggcgccgtcg
     1021 tctggcttgt cgtcgtcgcg gctacgctgc tcgccacgtt ggcgttcaag ttgatgttcc
     1081 tgtcatgctc gccgctgttc ggcccaatgg acgtgcatag cccaagcgga catgtcgcgg
     1141 cggcgacggt cgtgtgcggg gggctggccg cgatgctgac ccgtcatcgg gccaccattc
     1201 tgccggccgc attcctggcg gcgatcgtga tcggaatctc gcgcatcgtg ctgggcgcgc
     1261 actcgttgcc cgaggtggtt gtcggcgcct tgatcggcct actgggtgcg gcggcgctgt
     1321 tgcgcttcgc gggaccgccg ccacgtctga ggatcgcgcc gctgatcgtc gtgatcgtaa
     1381 tcatggcggg gatctttcac ggaatgcatc taccggccga ggcggcgatt cgccactccg
     1441 ccttccgcgc ggcgcagttc atcccggcct gtcgaggtgc tccggtgttc cagcatccgc
     1501 gggacgagac ccagctacct ccacaacctg ttcccatcgc tcctcgcctg gcgtagcctg
     1561 cttgcctgtc ggggcctgct tgcctgtcgg ggcgtgacgg caatgagggg gagacgatgc
     1621 ccggtacgac acgacgaggc ctgggcttgg gcttggctgc cacactagcc agccggtcgg
     1681 cccgcgctga cttggcggcg cttgaagccg ctgcgaggcg cgaaggttcg gtcacctggt
     1741 atgtcgcaca aatgagcggc gaggcagccg aggcaatggc acgtcgcttc acccagcgct
     1801 accccggcgt ctcggtctcg gcgatccgca cgaccggtca ggttgcctat gagcgtctgc
     1861 aacaggaact gaaaaacaac acgcccacct gcgacgtgtt cagttccacc ga
//
LOCUS       DASWQN010000159         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2128925, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000159 DASWQN010000000
VERSION     DASWQN010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2128925"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1334
                     /locus_tag="VGC82_02655"
     CDS             162..1334
                     /locus_tag="VGC82_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEY0422202.1"
                     /translation="MPIVNRVADLQPDIQAWRRDIHQHPELQYDVHRTAAFVADRLRE
                     FGCDEVATGLGRTGVVGVIKGRKPAGNRDIRVIGLRADMDALPIEEATSLPYASTTPG
                     KMHACGHDGHTAMLLGAARYLAETRNFAGDAVVIFQPAEEGGAGAAAMIEDGLFDRFA
                     IDQVYGMHNGPGIPVGSFAIRPGPIMAATDAIDIRIEGMGGHAARPHKCIDPVLAGAQ
                     LITALQGLVARNVDPLESAVISICEFHAGNARNVIPQTAELKGTVRTLTAEVRELVEK
                     RVREVVAGVAQVTGTKIDLEYTRGYPVVVNHGPQTDFATQVAREVAGDANVQDMAPLM
                     GAEDFAYMLEQRPGAFIFCGNGDSAGLHHPAYNFNDEAIVFGTSYWIKLVETSLAA"
     gene            1481..>1922
                     /locus_tag="VGC82_02660"
     CDS             1481..>1922
                     /locus_tag="VGC82_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0422203.1"
                     /translation="MTAKDKKLADLFLDTLKDIYFAEKQILKALPKMAKAAQSDKLRA
                     AFEKHRDETEGQIERLEKVFELIDKPARGKTCEAIQGILEEGKEIMDEFKGSDALDAG
                     LVSAAQAVEHYEIARYGTLKQWAQQLGMQDAVRLLEQTLQEEKKT"
BASE COUNT          374 a          605 c          618 g          325 t
ORIGIN      
        1 cttgcagtct tctcggcgtt tcagaattcc gcggcgtcaa accgtctctc ctatctcccc
       61 ggcagaaagc ccggccatgg gggcctttgt caatcaccgt tggggggatc gcggccgcgt
      121 gctacaccgg ggaaaacctc acccatgacc ccgagatttc catgcccatc gtcaaccgcg
      181 tcgccgatct gcaacccgac attcaggcct ggcgccggga tatccaccag catccggagt
      241 tgcagtatga cgtgcatcgc acggcagcat tcgttgcgga tcggttgcgg gaattcggct
      301 gcgacgaggt ggctaccggt ctcggccgca ccggcgtcgt cggcgtcatc aagggccgca
      361 agcctgccgg caaccgcgat atcagagtga tcgggctgcg cgccgacatg gacgccctgc
      421 cgatcgagga ggcgaccagt ctgccttacg cctccacgac gccgggcaaa atgcacgcct
      481 gcggccatga cggacatacc gcaatgctgc tcggcgccgc gcgttatctt gcggaaacgc
      541 gcaatttcgc cggcgatgca gtcgtgatct tccaacccgc ggaggagggc ggcgccggtg
      601 ccgcggcgat gatcgaggac gggctgttcg accgttttgc aatcgaccag gtgtacggca
      661 tgcataacgg cccgggaatc ccggtcggct cgttcgcgat ccgccccggc ccgatcatgg
      721 ccgcgaccga cgccatcgac atccggatcg aagggatggg cggccatgcg gcccggccgc
      781 acaaatgcat cgatcccgtg ctggcggggg cccagctgat tacggcgctg cagggcctgg
      841 tggcacgtaa cgtggacccg ctggaatcgg ctgtcatctc gatatgcgaa ttccacgccg
      901 gcaatgcgcg gaacgtgatt ccgcaaaccg ccgagctgaa aggtaccgtc cgcaccctga
      961 ccgccgaagt ccgggaactg gtcgagaagc gggtccgcga ggtcgtggcc ggcgtggcgc
     1021 aggtcaccgg caccaagatc gacctggaat acacccgcgg ctatccagtg gtggtcaacc
     1081 atggcccgca gaccgatttc gcgacccagg tggccaggga agtggccggc gatgccaacg
     1141 tccaggatat ggcgccgctg atgggcgccg aggatttcgc ctacatgctg gagcagcggc
     1201 cgggcgcctt cattttctgc ggcaacggcg acagcgccgg cctgcaccac cccgcctata
     1261 atttcaacga cgaggccatc gtgttcggca cctcgtactg gatcaagctg gtcgagacct
     1321 cgctggcggc gtgagcctcg cggtggtttg ttcgctgcgc cgacggcctg gattggccgc
     1381 cgcaggctct tttgcagttc ccggaacatt gctctggagg gcacgttgga aggcggggga
     1441 ccttcccaaa tcccagtgac atcctacgat ggagtttgcc atgaccgcca aagacaagaa
     1501 gcttgccgac ctgttccttg atacgctcaa ggacatctat ttcgccgaaa agcagatttt
     1561 gaaggcgctt ccgaagatgg cgaaagccgc gcagtccgac aagctgcgcg ccgccttcga
     1621 aaagcaccgc gacgagaccg aaggccagat cgagcgtctc gagaaggtct ttgaattgat
     1681 cgacaagccg gcgcgcggca agacctgcga ggcgatccag ggcatcctcg aagagggcaa
     1741 ggagatcatg gacgagttca aaggtagcga cgcgctggac gccggcctgg tttccgccgc
     1801 gcaggccgtc gagcactacg agatcgcccg ctatggcacg ttgaagcagt gggcacagca
     1861 gcttggcatg caggatgcgg tgcgactgtt agaacagacc ctgcaggagg aaaagaagac
     1921 cg
//
LOCUS       DASWQN010000160         4147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_677113, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000160 DASWQN010000000
VERSION     DASWQN010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677113"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2091)
                     /locus_tag="VGC82_02665"
     CDS             complement(<1..2091)
                     /locus_tag="VGC82_02665"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY0422204.1"
                     /translation="MIAAQLLIIICGLAALGYGLITGRQVLAADAGTARMQEISAAVQ
                     EGARAYLNRQYTTIGIVGVVILVLLALVLGAHTAVGFAIGAVLSAAAGYVGMNVSVRA
                     NVRTAQASRRGLAAGLDVAFKAGAITGMLVVGLGLLGVAGYYFYLRAGYSSAMTDDQL
                     RKVLEAMVALGFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIA
                     DNVGDNVGDCAGMAADLFETYAVTIVATMLLAGIFFTGATRDALLMLPLGICAICILT
                     SIAGTYFVKLGPDNGIMWALYKGLLVTGGLSVVGVALIILITVGFTAPLELVGGGAID
                     GWQLFLCALAGLAVTGLIVVITEYYTSTEYRPVKSIAQSSTTGHGTNVIQGLAISMEA
                     TALPVIVISIGIIFAFMVAGLFGIAVAATTMLSLAGMIVALDAYGPVTDNAGGIAEMA
                     DLPKEVRVTTDALDAVGNTTKAVTKGYAIGSAGLASLVLFAAYTQDLTHYFPALTVDF
                     SLTNPFVVIGLFIGGLLPYLFGAMGMTAVGRAAGAVVVEVRRQFREIPGIMEGTAKPE
                     YGKAVDLLTKAAIKEMILPSLLPIGAPIVLWVVIDIIAGRSAAFASLGAMLLGTIVTG
                     LFVAISMTSGGGAWDNAKKYIEEGNHGGKGSEAHKAAVTGDTVGDPYKDTAGPAVNPM
                     IKITNIVALLMLAIL"
     gene            complement(2344..2517)
                     /locus_tag="VGC82_02670"
                     /pseudo
     CDS             complement(2344..2517)
                     /locus_tag="VGC82_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            2644..4077
                     /locus_tag="VGC82_02675"
     CDS             2644..4077
                     /locus_tag="VGC82_02675"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEY0422205.1"
                     /translation="MCAMTKHRIVPVILSGGSGTRLWPVSRESFPKQLWPLVSERTMM
                     QETALRAASAEFAPPIVVCNNEHRFLIAEQLRAAGIADARIMLEPVGRNSAPAIAAAA
                     VLVAEEDPDAVLWMMAADASIADNAALQVALQAAAKAARAGRVVTFGMKPTAPETGYG
                     YIEIGAPMAGVEDVFEVARFLEKPDAKTAARFVSSGQHLWNSGMFVFTAGTLLEELDR
                     HAPEVLSAVRRAVTARTADLDFIRLDVAAFTACPSISIDYAVAERTSRAAVVPADLGW
                     SDVGSWSALWELGTKDAAGNVALGDVVLEGAENCYVRSDGMLAAVVGLKDAVVVVTED
                     AVLAMHRDLAQDVKKIVQRLKAAGRPEAVAHNRSYRPWGYYESLIKGDRFQVKRIVVT
                     PGRQLSLQSHFHRAEHWVVVNGTARVTRDAETVILRENESIYLPLGCQHRLENPGRIP
                     LTLIEVQSGAYLGEDDIVRLQDTYGRT"
BASE COUNT          769 a         1351 c         1319 g          708 t
ORIGIN      
        1 cagaattgcc agcatcagta acgccacgat gttggtgatc ttgatcatcg ggttcaccgc
       61 cggtcccgcg gtgtccttgt acgggtcgcc cacggtatcg ccggtcaccg ccgccttgtg
      121 cgcttcggag cctttgccgc catggttgcc ctcctcgata tacttcttgg cgttatccca
      181 cgcgccgccg cccgaggtca tcgagatcgc gacgaacagg cccgtcacga tggtgcccag
      241 cagcatcgcg cccagcgaag cgaacgccgc cgaccggccg gcgatgatgt cgatcaccac
      301 ccacagcaca atcggggcgc cgatcggcag cagggacggc aggatcattt ccttgatcgc
      361 cgccttggtc agcaggtcca ccgctttacc atattccggc ttggcggtgc cttccatgat
      421 gcctggtatc tcgcggaact ggcggcgaac ctccaccacg accgcgccgg ccgcacgtcc
      481 taccgccgtc atgcccatcg cgccgaacag gtacggcagc aggccaccga tgaacagtcc
      541 gatcaccacg aacgggttgg tcaggctgaa atcgaccgtc agggcgggaa aatagtgcgt
      601 caggtcctgg gtgtaggccg cgaacaacac cagtgacgca aggccggcgg acccgatggc
      661 atagcctttg gtcaccgcct tggtagtgtt tcccaccgcg tccagcgcat ccgtcgtgac
      721 ccgcacttct ttcggcagat ccgccatttc cgcgatgccg ccggcgttgt ccgtcaccgg
      781 cccataagcg tccagcgcca cgatcattcc ggccagcgac agcatggtcg tcgccgcaac
      841 ggcgatgccg aacagccccg ccaccatgaa ggcgaagatg atgccgatgc tgatcacgat
      901 caccggcaac gccgtcgctt ccatcgaaat cgccaacccc tggatcacgt tggtgccgtg
      961 accggtggtc gagctttgcg cgatggactt caccgggcgg tattcggtcg aggtgtaata
     1021 ctccgtgatc accacgatca ggcctgtaac ggccaggccg gccaaggcgc aaagaaacag
     1081 ctgccacccg tcgatcgccc cgccgccgac cagttccagc ggcgcggtaa agccgaccgt
     1141 gatcaggatg atcaaggcga ctccaaccac ggaaagtccg ccggtaacga gcaagccctt
     1201 gtacagcgcc cacatgatcc cgttatccgg tcccaatttc acgaagtagg tgccggcgat
     1261 ggaggtcagg atacagatcg cgcaaatgcc aagcggcagc atcagcaaag cgtcacgggt
     1321 cgcgccggtg aagaaaatcc ccgccagcag catcgtggcg acgatggtca cggcataagt
     1381 ctcgaacaaa tccgcggcca tgccggcgca gtcgccgaca ttgtctccga cgttgtcggc
     1441 gatcaccgcc ggattgcggg gatcatcctc gggaatgccg gcttctacct tgcccaccag
     1501 gtcggcaccc acgtcggcgc ccttggtgaa aatgccgccg ccgagacggg cgaagatgga
     1561 gatcagcgag ccgccaaacc ccagcgcgac catggcttcc aacaccttgc gaagttggtc
     1621 gtcggtcatc gccgaactgt atccggcacg cagatagaag tagtagccgg ccacgcccag
     1681 cagacccagg ccgaccacca gcatgccggt aatggcgccg gccttgaagg ccacgtccaa
     1741 tccggcagcc aaacccctgc gggacgcctg tgcggtgcgc acgttggcgc gaaccgatac
     1801 gttcatgccg acataaccgg cggcagccga cagcaccgca ccgatggcaa accccacagc
     1861 ggtgtgggcg cccagaacca gggccagcag caccaggatg accacgccga cgatgccgat
     1921 ggtggtgtat tgccggttca agtaggcgcg cgcgccttcc tggacggcag ccgaaatctc
     1981 ctgcatgcgg gccgttccgg catccgccgc gagaacctgc cggccggtga tcagaccata
     2041 acccaaagcc gcaaggccgc agattatgat cagcagttgc gccgcaatca tcagggtttt
     2101 cccctcctca tggtttcgct taggttcgcc cgcaagaccg ggaacgcggg cgcgaccgtg
     2161 gccgcggcgt atgccagaac caaaggcatc cggcaaatcg gcgttcaacg tttattataa
     2221 cggatgcggt cgccgtcacg cggcagactc cgcgctggat cgggcatcgg acgatggatc
     2281 aggccccccg gggatcaggc ctcctgggga tcaggcctcc tggagatcag gcccccaaag
     2341 ggatcaggcc aaacttgcca ccacttcctg ttccgtcgga aaacggcccg tcgccttctt
     2401 ggaaaaggct gggcgaccgt ccacgacgat ctcgaaaacc ccgccgccgc ttttgcgcaa
     2461 ggcaacccgg gcggtcgggt cggttttcag gatcaggtcc cgtgccgcta tcgcgcgcgg
     2521 ttcgtagcct cacattccgc agtattcgat ttctatccgc atcttttgaa catcctttgc
     2581 cgttgatggc gcttctagct tggcagcgat gccgtggttg ggcaagaaag gccgcgtatc
     2641 tggatgtgcg ccatgaccaa acaccgtatt gttcctgtta tattgtccgg cggctccggc
     2701 acgcggctgt ggccggtctc gcgggagagc tttccaaagc agctttggcc gttggtgtcg
     2761 gagcggacga tgatgcagga aaccgcgctg cgggctgcct cggccgagtt cgccccgccc
     2821 atcgtggtgt gcaacaacga acacaggttt ctcatcgccg aacagcttcg ggccgcaggg
     2881 attgccgacg cccggatcat gctggagccg gtcggccgca acagcgcccc ggcgatcgcc
     2941 gccgcggccg ttctggtggc ggaagaggac cccgacgcgg tgttgtggat gatggcggcc
     3001 gacgcttcga tcgccgacaa cgcggctttg caggtggcac ttcaggccgc cgcaaaggcc
     3061 gcccgcgccg ggcgcgtggt gacgtttggc atgaagccga ccgcaccgga gaccggctat
     3121 ggctacatcg agatcggtgc gccgatggcc ggcgtcgagg acgtcttcga ggtggcccgt
     3181 ttcctcgaga aacccgatgc gaaaaccgcc gcgcgcttcg tctcgagcgg ccaacacctt
     3241 tggaactccg gcatgttcgt cttcaccgcc ggcaccttgc tggaggaact ggaccgccac
     3301 gcgccggagg tgttgtccgc ggttcgccgg gcggtcacgg cgcggacggc ggatctggat
     3361 ttcatccgcc tggatgtcgc ggccttcacc gcctgcccct cgatcagcat cgactacgcg
     3421 gtggccgaac ggaccagccg ggcagcggtg gtgccggcgg atttgggctg gtcggatgtc
     3481 ggaagttgga gcgcgctttg ggaactcggc acgaaggacg ccgccggcaa cgtggcgctg
     3541 ggcgacgtgg tgctggaggg agcggagaat tgctatgtcc gcagcgacgg catgctggcc
     3601 gccgtggtcg gactgaagga tgccgtggtg gtggtgaccg aggacgcggt tctggccatg
     3661 catcgcgacc tggcccagga cgtcaagaag atcgtccagc gcctgaaagc cgccggccgg
     3721 cccgaggcgg tggcgcataa ccgcagctac cgcccctggg ggtactacga gagcctgatc
     3781 aagggcgacc gcttccaggt caagcggatc gtggtgacgc cgggccggca gttgtcgctg
     3841 caaagccatt tccatcgggc ggagcattgg gtcgtggtga acggcacagc gcgggtcacg
     3901 cgcgatgcgg agaccgtcat cctgcgtgag aacgagagca tttacctgcc gcttgggtgt
     3961 caacaccggc tggaaaaccc cggccggatt ccgctgacac tgatcgaggt gcaatcgggc
     4021 gcctaccttg gcgaagacga catcgtccgg cttcaggaca cgtacggacg gacgtagacc
     4081 tgtccgtgcc cgtccgcctc agggcggatt tcgacgggcg agcggttcga tgaactgggg
     4141 ttcggtg
//
LOCUS       DASWQN010000161         3151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1337002, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000161 DASWQN010000000
VERSION     DASWQN010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337002"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..965)
                     /locus_tag="VGC82_02680"
     CDS             complement(255..965)
                     /locus_tag="VGC82_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0422206.1"
                     /translation="MGDGWAAEATPGPRTAILFAGDPHGNFAPILRACTQHPPGTLIL
                     VGDCDLPSRLDRVFAPAIGQGWDVCWILGNHDTATEASYDNLTGCPGDLGLGVTTIGG
                     ARIAGLPGVFKPRVWTPDEAPAFVTRSAFLANLRPGEAWRGGLPLFHRDTIFPEDFDR
                     LARLRCDILVTHEAPSSHPHGFHAIDALAQACGARLIVHGHHHQSYAAVLPNGIAVRG
                     LGLAEPWFHAPAVPGTFS"
     gene            complement(958..2229)
                     /locus_tag="VGC82_02685"
     CDS             complement(958..2229)
                     /locus_tag="VGC82_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0422207.1"
                     /translation="MTNDLISVTVKAAVCLIQDERLDPLDFMEAYLDRIASHEPTIKA
                     FAHFDPTQARAAAARARPGPLQGIPIGVKDVLDTADMPSQYGSPIWAGHQPKADAAPV
                     AWARAAGAVVIGKTVTTEFATRKPGPTMNPANPGHTPGGSSSGSAAGVAAGLFPLAYG
                     TQTAGSIIRPAAFCGAVGYKPSFGMINRTGMKLMSDSLDTVGTIARTVADCALFAGAV
                     SGRDLGDPDIKPDATPRIGICQSPAWHTAAPETTALLARVTQALSRAGASVSQRELPS
                     SFNALVEAHPIIMNNESARALGWELAHHPEQISEGLRERLAFGLSQSQSALENAYSVF
                     RNTQSAFPGAMGGLDVLVTPSAPGEAPKGLDWTGDPAFNAIWTSLHVPCVTVPAGTGP
                     NGLPLGIQIVGRRGDDRAVLAWARWVEAAIG"
     gene            2662..>3151
                     /locus_tag="VGC82_02690"
     CDS             2662..>3151
                     /locus_tag="VGC82_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEY0422208.1"
                     /translation="MFLRQTRRTKDGKTHSYWSVVENQRLDGGRVVQRPVLYLGEINS
                     SQATVWRKAIEVLDEDAGRPQTLALFPEDRAAAVVSDNAVVQLRLSEMRLCRPRQWSA
                     CWLARQLWGELQLDQFWSDRLPPNRKGTRWDQVLQVLASYRLIAPGSEWKLHRDWFGR
                     SAM"
BASE COUNT          560 a          946 c         1079 g          566 t
ORIGIN      
        1 ataccatggg tgtaacggca gattgcagac gcagacagtg cggcaccgaa cgaaacaacg
       61 ccgagttccg gtgaacccgc gatagtttgc cggccaaccg tgcgcgggga ttcgtttctt
      121 gcgccgtgat ccacgcatcc cagccgatgt ccaaccgacc acgccggcct gcgaaagctt
      181 agttgcctgg ggctgaacag gactggcatc acagcgcctt cggcgcaatt gcggtggcgc
      241 gcgtcaatcg atcgttatga gaaggtgccc ggaaccgctg gtgcgtggaa ccatggttcg
      301 gccagcccga gaccgcggac ggcaatcccg ttcggcagga ccgccgcgta ggactgatgg
      361 tggtggccat gcacgatcag gcgcgcgccg cacgcttggg ccagtgcatc gattgcatgg
      421 aagccgtgcg ggtgactgga cggggcttca tgggtcacca gtatgtcgca acggagcctg
      481 gccagacggt cgaaatcctc ggggaagatc gtgtcgcggt ggaacagcgg caagccgccg
      541 cgccaagcct cacccggacg caaattggcc aggaaggcgc ttcgggtcac gaacgcgggc
      601 gcttcatccg gcgtccagac tctcggcttg aacacgcccg gcagaccggc gattcgagcc
      661 ccgccgatcg tggtgacgcc caggcccaga tcgccggggc aaccggtcag gttgtcatag
      721 gatgcctcgg tcgcggtgtc gtggttgccg aggatccagc atacgtccca gccctgcccg
      781 attgcaggcg cgaagacccg gtccaggcga gaaggcagat cgcagtcgcc gacgagtatc
      841 agcgtgccag gggggtgctg ggtgcatgcc cgcaagatag gcgcgaagtt tccgtgcgga
      901 tcaccggcga acaggatcgc ggtgcgggga cctggagtcg cctcagccgc ccaaccgtca
      961 cccaatcgcc gcctccaccc agcgcgccca cgccagcacc gcccgatcgt cgccccggcg
     1021 gccgacgatt tggataccga gcggcaggcc gttcgggccg gtgccggcgg ggacggtgac
     1081 gcagggaacg tgcaacgagg tccatatggc gttgaacgcc gggtcgccgg tccagtccag
     1141 gcctttgggc gcctcgcccg gtgccgaagg cgtcaccagc acatccagcc cgcccattgc
     1201 gccggggaac gcggattggg tatttcggaa gacggaatag gcgttttcga gtgcggactg
     1261 gctttggctc aggccgaacg caaggcgttc gcgcaagcct tcgctgatct gctccggatg
     1321 gtgcgccagt tcccagccca gtgcgcgggc gctttcgttg ttcatgatga tgggatgggc
     1381 ctcaaccagg gcattgaagc tgctgggcag ttcgcgttgg ctgaccgagg cgccggcgcg
     1441 gctgagcgcc tgcgtgacgc gagccagcaa agctgttgtt tccggcgccg cggtatgcca
     1501 ggcaggcgac tggcagatac cgatccgcgg cgtggcgtcc ggcttgatgt cgggatcacc
     1561 gaggtcgcgg ccggatacgg cgccggcgaa cagcgcacag tcggcgacgg tgcgagcgat
     1621 cgtgccgact gtgtccaggc tgtcggacat cagcttcatg ccggttcggt tgatcatccc
     1681 aaaactgggc ttgtagccga cggcaccgca gaacgccgcg ggacggatta tgctgccggc
     1741 ggtttgcgtg ccgtaggcca gcgggaacag gccagcggca acgccggcgg ccgaccctga
     1801 ggacgatccg cccggcgtat ggccggggtt cgccgggttc atggtgggtc cgggctttcg
     1861 cgtcgcgaac tcggtggtga cggttttgcc gatcacgaca gcacccgcgg cccgtgccca
     1921 ggcaacgggg gcggcgtcgg ctttgggttg gtgaccggcc cagatcgggg agccgtattg
     1981 gctgggcatg tcggcggtgt ccagcacgtc ctttacgccg atgggaattc cctgcaacgg
     2041 accggggcgt gcccttgcag cggctgctcg cgcttgggtg gggtcgaaat gtgcgaaggc
     2101 ctttatggtt ggctcatggc tcgcgatgcg gtcgagatag gcttccataa aatccagcgg
     2161 gtccagccgc tcatcctgga tcaagcaaac tgctgctttc acggtaacgc tgatgagatc
     2221 gttcgtcata ggcaaagctc catttttggc gcagggcaac accagcgtgg cggcgcgtca
     2281 actcgtcggt cagaaggatg tggacctttg aacaccctaa gccggtgaaa acgaccggcc
     2341 ggttcggtgt cagcctacag cgatgtcaca cgaccggcca actcgatggg tccgaataat
     2401 cctggatgaa tgcctgttcg ataggcttaa gatcgaattc ttccgtcaag ctggtagtat
     2461 aatccgaaat catgtcataa caaactgcta tcttagcccg ctttcgcgga ttcgcccccg
     2521 gaaaatcgat tttagcccga atggctccgg gtaaacctat gaccttgcgc tgttatatgg
     2581 agcggatgcg gccgaaaacg gctgtcgtga agacctaccc tgttccaagg agcagattct
     2641 gttcgctaga ctcgccgggt catgtttttg cggcagaccc ggcgaacgaa ggacggcaag
     2701 acgcatagct actggagcgt cgtcgagaac cagcgtctcg atggtggccg cgtggtgcag
     2761 cgccccgtgc tgtatctggg tgagatcaac tcgtcgcagg cgacggtatg gcgcaaggcg
     2821 atcgaggttc tcgatgagga tgccgggcgt ccgcagacac tggcgctgtt ccccgaggat
     2881 cgcgccgctg cggtcgtgtc cgacaacgcg gtcgtgcagc tccgcctgtc cgaaatgcgg
     2941 ctgtgccggc cgcgacaatg gagtgcgtgc tggctggccc ggcagttgtg gggcgagttg
     3001 caacttgatc agttctggtc cgatcgcctg ccaccgaacc gcaagggcac gcgatgggat
     3061 caggtcttgc aggtgctggc gtcgtaccgg ctgattgcgc cgggcagcga atggaagctg
     3121 catcgcgact ggttcggccg cagcgccatg g
//
LOCUS       DASWQN010000162         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2392764, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000162 DASWQN010000000
VERSION     DASWQN010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392764"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1885)
                     /gene="treY"
                     /locus_tag="VGC82_02695"
     CDS             complement(<1..1885)
                     /gene="treY"
                     /locus_tag="VGC82_02695"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEY0422209.1"
                     /translation="MTPRATLRLQLHKAFTFSDAAALVPYLADLGISHVYSSPILTAR
                     AGSIHGYDVVDPTSVNPELGGEPGFREFVAALRARGLGLIVDIVPNHMAVGGSDNPWW
                     TDLLRHGRASRYADFFDVDWETDDPDLRGKILAPFLGRPYGEALDAGEIRLAHSSSGE
                     PVVTYFDSTFPIDPSDYAHIAECGLESFDPGNPSGRCQIHTLLERQHYRLAWWAIAGD
                     EINWRRFFDINGLAGLRIEVPAVFEETHATLFRLYADGLIDGFRVDHVDGLSDPPGYC
                     RRLRQRLQELAPDRYAYLVIEKILGAGESLPTDWGVDGTSGYDFMNEVSALQHDPGSA
                     AALGRLWHQLTQRPAEFEEEEVAARREILRSGFDAQLKAVTAALHRIARSDRHTRDFS
                     AAKIRDGLVNLLAHFPVYRGYDAGSKRSRMDAASFVKALKAAKQSSPESTHKVLDQLD
                     RWLGGEPGNKVANTRFQQLSAPVAAKSVEDTAFYRYGRLLSRNDVGFDAARLGGSVAD
                     FHRACADRLATFPHAMLATATHDHKRGEDLRARLAVISEVPEEWTAFLEQCGAARSSG
                     LDPADEIMLYQMIVGAWPPELDYSDAAGCRAFAERLAAWQQKALREAKLHTNWTAPNE
                     TYER"
     gene            complement(1882..>2675)
                     /locus_tag="VGC82_02700"
     CDS             complement(1882..>2675)
                     /locus_tag="VGC82_02700"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY0422210.1"
                     /translation="EENGFAPFLSTLRAAMRHAGGIRVDHAMGLARLWLIPEGASPAD
                     GAYLNFPVGDLLRLLALESVRHNVVVIGEDLGTVPEGFHDMLEQSGIHGMRVLWFERD
                     AQTGFIPPRGWGSTDVAMTSTHDLPTVAGWWKGIDIDVRHKHGKLGEGVDPDAMHKER
                     EQDRPKLWNAFVREQVGEGSVPTPDETDRVVDAAVRYIAKTEVPLSLIPLEDLIGQVE
                     QPNLPGTVQEHPNWRRRLPMQADAVLETEAVARRIDFVAAERPRQ"
BASE COUNT          499 a          882 c          806 g          488 t
ORIGIN      
        1 tcctttcata ggtttcgttc ggcgccgtcc agttcgtgtg cagcttggct tcgcggaggg
       61 ccttttgctg ccacgcggcc agtcgttcgg cgaacgccct acatcctgct gcatcggaat
      121 aatccagttc cggtggccag gcaccgacga tcatctgata cagcatgatc tcatcggcgg
      181 gatcgaggcc ggaagatctg gccgcgccac actgctccag gaatgcagtc cattcttcgg
      241 gaacttcgct gataaccgcc agccgagcgc ggagatcctc gccacgctta tggtcatggg
      301 tagcggtggc cagcatggcg tgcggaaaag ttgccaacct gtccgcgcag gcccgatgaa
      361 aatccgctac cgatccgcca agccgcgccg catcgaagcc gacatcgttg cgcgacagca
      421 ggcgaccata gcgatagaag gcggtgtcct cgacggattt cgccgcgacc ggcgcgctaa
      481 gctgctgaaa tcgcgtgttg gcgaccttgt ttcccggttc accgcccagc caacggtcca
      541 actgatccag aactttgtgc gtcgactccg gcgaagattg tttggccgcc ttcaacgctt
      601 tcacgaacga cgcggcgtcc atcctggacc gcttcgaccc ggcgtcatag ccgcgataga
      661 ccgggaaatg cgccagcagg ttcaccaggc catcacgaat tttcgccgcg gaaaaatcgc
      721 gggtgtgacg atcggagcgg gcgatgcgat gcagcgccgc ggtgacggcc ttcagttggg
      781 cgtcaaagcc ggaccgcaat atttcccgcc tggcggcgac ttcctcctcc tcgaattccg
      841 ccggccgctg agtaagctgg tgccacaacc gcccgagtgc cgcggcgctg ccggggtcat
      901 gctgcagcgc acttacctcg ttcatgaagt cgtagccgct ggtcccgtcc acgccccaat
      961 cggtcggcaa cgactctccc gcgccgagga ttttctcgat caccagataa gcgtaccggt
     1021 cgggcgccag ttcctgcagc cgctggcgca gccggcggca gtaacctggc ggatcggaaa
     1081 gtccgtccac atgatcgacc cggaagccgt cgatcaagcc gtcggcgtag aggcggaaca
     1141 aggtcgcatg ggtttcctcg aacaccgccg gcacctcgat ccgcaggcca gcgaggccgt
     1201 tgatgtcgaa gaaccgccgc cagttgatct cgtctccggc gatagcccac cacgcgaggc
     1261 gataatgctg ccgctccagc agcgtatgga tctggcagcg tccactcgga ttgcccgggt
     1321 caaaggactc cagcccgcat tcggcaatgt gcgcgtagtc gctgggatcg ataggaaagg
     1381 tgctatcgaa ataggtgacg accggctcgc cggatgaact atgcgccaag cggatttccc
     1441 cggcgtccaa tgcttcgccg taagggcggc caaggaacgg tgccaggatt ttcccgcgca
     1501 gatcggggtc atcggtttcc cagtccacgt cgaagaagtc cgcgtagcgg ctggctcgtc
     1561 cgtgccgcag caagtccgtc caccacgggt tgtcggagcc gccgaccgcc atgtgattcg
     1621 gcacgatatc gacaatcagg cccaggccgc gggcgcgcag cgcggcgacg aattcacgga
     1681 accccggttc cccacccagt tccggattga ccgaggttgg atcgaccaca tcgtagccgt
     1741 ggatggaccc cgctcgggcc gtcaggattg gggacgaata gacgtggctg atcccaagat
     1801 cggcaaggta agggaccaag gcggccgcgt cggagaaagt gaaggccttg tgaagttgca
     1861 gccgcagggt cgcacgaggt gtcattggcg cggcctttcc gcagccacga agtcgatgcg
     1921 ccgtgccacc gcctcggttt ccagcaccgc atccgcctgc atcggcaagc ggcggcgcca
     1981 gttcggatgt tcctgtaccg tcccgggaag attcggctgc tcaacctgtc ctatcagatc
     2041 ctcgagcgga atcagggata gcggaacttc ggttttggcg atgtagcgca ccgcggcatc
     2101 gacgacgcga tccgtctcgt ccggcgtcgg caccgatccc tcgccaacct gttcgcgcac
     2161 gaaggcattc caaagcttcg gccgatcctg ttcacgttcc ttatgcatcg cgtccggatc
     2221 gacgccctcg ccgagcttgc catgcttgtg gcgcacatcg atatcgatgc ccttccacca
     2281 acctgctacc gtcggcaaat catgcgtgga cgtcatcgcc acgtcggtac tgccccagcc
     2341 gcgcggcggg ataaatccgg tctgtgcgtc gcgttcgaac cacaggacgc gcatcccatg
     2401 tatcccgctt tgttcgagca tatcgtggaa accttcggga acggttccca ggtcttcgcc
     2461 gatcacgacg acgttgtgcc gcaccgattc cagcgccagc aaccgcagca ggtctccgac
     2521 cgggaagttc agataggcgc catccgccgg ggaggcgcct tcgggtatca gccacagccg
     2581 cgccaagccc atcgcatgat cgacccttat cccgccggca tgacgcatgg ccgcccgcag
     2641 cgtcgacagg aacggcgcaa agccattttc ctcca
//
LOCUS       DASWQN010000163         4839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_149726, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000163 DASWQN010000000
VERSION     DASWQN010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149726"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VGC82_02705"
     CDS             <1..412
                     /locus_tag="VGC82_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422211.1"
                     /translation="IEARLLDNSRAAGRPKFLTYHKLDVIRHRAARQPARGAWQLLDL
                     AAAGLLAAARAPILSAMFVVTEADTAAIRDVFERDGELSAAIELRRRFPGITDNAQAR
                     ACVRTIARWTPQASPPPKVTRLRQPGRQPGPESG"
     gene            587..1798
                     /locus_tag="VGC82_02710"
     CDS             587..1798
                     /locus_tag="VGC82_02710"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0422212.1"
                     /translation="MTETSPSPLSLPLAGVTVIDFGQIFQGSYASLLMAKAGANVIKI
                     EPPHGEPLRQRAPPGRNSTLPFAMLNANKRGITLNLKSARGRELLLEMVRRADVLLEN
                     FAPGAMDALGVGWSVLHEVNPRLIYATGTGYGISGADKDNLAMDLTVQAASGIMSVTG
                     FPDRQPVRAGITVADFMGGTHLYAGIVTALYERDRSGVGRLVEIAMQEAVYYTLAAAF
                     EQLSRTGKVPPRTGNDSGGSAAPYGLYPLKDGLLAIHPGTEQHWRNILKAAGRDDLLE
                     DPKFRTMHDRSRHLDEVTAIVTAWTSQLTKEEATEQARRFRIPCSPVRDVSEVMHDPH
                     MHARGMLEWVEHPDLGRVVLPTTPIRLHDTAVAATMPSPRLGQNNDDVYGGWLGLAAD
                     EIVALRAEGVI"
     gene            2061..2288
                     /locus_tag="VGC82_02715"
     CDS             2061..2288
                     /locus_tag="VGC82_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY0422213.1"
                     /translation="MPDGRLSLPAGLRKKHGLIKGGEVIVEDTGDAIMLRTFDQVVAR
                     AQALSRKLVEGKAGSSVDDFLSDRAREAEAE"
     gene            2285..2674
                     /locus_tag="VGC82_02720"
     CDS             2285..2674
                     /locus_tag="VGC82_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0422214.1"
                     /translation="MTDVVLDASALLALLLDEPGGDKVKAMLDGALLGAVNLAEIVSH
                     YAKLGAARSDIEALLQPLPVRVVPIDERLSYEAGMLRPVTLKAGLSLGDRYCLALAKR
                     EGVPAVTAERRWPDIAAAAGVTVDLIR"
     gene            3089..4669
                     /locus_tag="VGC82_02725"
     CDS             3089..4669
                     /locus_tag="VGC82_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0422215.1"
                     /translation="MSTSPNLSALSRPKLEALTVEVMDENAALKQMVNQQREEIAWLK
                     GLKGRPDIKPSGMEKGTEPAKPSRQDKRPTRGKVRPRVNVEDRIIKAIVPPGSRFKGY
                     ETYQVQDLVLAVHAVRYCRERWVTPEGKTIIAPLPEGTRGHFGPDLRRFVLMQYHQGQ
                     TTLPRLATLLQSLGVAISEREIQRLLTEGLEQFLVENRDVLRTGLQTSSWISVDDTGA
                     RHQAKNGYCTQIGNQHFTWFGTRSSKSRLNFLDLLRAGHSDFMLNDAAFDYMRSRALP
                     ASLIARLAAAADTIFADQAAWSAHLARLGFTGLTTTPDPVQIATEGAIWGSVHAHDFL
                     RNAVVLSDDAGQFAVGRHALCWVHAERLVHKLDTFTDQHRDAQQTVRKLIWDFYADLK
                     AYQANPDKRCRPALRARFDRIFRRRTGFVTLDRLLARLHANKAELLMVLERPEIPLHT
                     NGSENDIRCQATRRKVSAGTRSDLGRDCRNAFLGLGKTCAKLGVAVWDFLGNRLGVPG
                     HLLVPSLPELIRCRGQPA"
BASE COUNT          914 a         1535 c         1541 g          849 t
ORIGIN      
        1 tatcgaagca agattgctag acaatagtcg tgctgccggt cgcccgaagt ttctgactta
       61 ccataaactg gacgttatac ggcaccgggc ggcacggcag ccggcgcgcg gcgcttggca
      121 gctgctcgat ctcgcggctg ccggcttgct tgccgcggcc cgcgcgccca tcctttccgc
      181 catgtttgtc gtcactgaag ccgacaccgc tgccatccgc gatgtcttcg agcgggacgg
      241 tgagctatcc gcggcgatcg agctccgccg ccggtttcct ggcatcaccg acaacgccca
      301 ggcgcgggcg tgcgtgcgga cgatcgccag atggacgccg caggcttcac caccgcccaa
      361 ggtgacgcgg ttgcggcagc ccggacgcca gcccggcccc gagagcggct aacgcgacgc
      421 gcgcgtggtc ggggcagggg cggcggccgc ttcatcacga cgaagccgga ctgctccagg
      481 tggtgaagcc gagatagcct cccccgctcc tgctgcctta gcatgcatgc cgttttggac
      541 agatcgcgta gcaaacctga ggaatcgaag ttttggaacg tcaagaatga ccgagacgtc
      601 accatccccg ctgtccctgc cgcttgccgg tgtgacagtt atcgacttcg gtcagatctt
      661 tcagggctcc tacgccagcc tcttgatggc gaaggccggg gccaatgtga tcaagatcga
      721 gccgccacat ggcgagcccc tacgccagcg cgcaccgccc ggccgcaaca gcacactccc
      781 ctttgccatg ctgaacgcca acaagcgcgg catcacgctt aacctgaagt ctgcccgcgg
      841 gcgggagctg ctgttggaaa tggtgcgccg ggccgatgtg ctgctggaaa atttcgcccc
      901 gggcgcgatg gatgcgttgg gtgtcggctg gtccgttctg catgaggtca atccacgcct
      961 gatctatgcg accggcaccg gctatgggat ttccggggcg gacaaggaca atctggcgat
     1021 ggacctgacc gtgcaggccg cctcggggat catgagtgtc accgggttcc cggaccgcca
     1081 accggttcga gccggcatca cggtcgcaga tttcatgggc ggcacgcatc tttatgctgg
     1141 gatcgtgacg gcgctctacg agcgcgaccg cagcggtgtg ggccgactgg tggagatcgc
     1201 catgcaggag gctgtgtact acaccctcgc ggcggcgttt gagcagttga gccggacagg
     1261 gaaggtcccc ccgcgcaccg gcaacgactc cggcggctcg gccgcaccct acgggctcta
     1321 cccgctgaag gatggcttgc tggcgatcca cccaggaacc gagcagcact ggcgcaacat
     1381 cctgaaggcc gcgggacggg atgacctact ggaggatccg aagttccgga ccatgcatga
     1441 ccgctcccgt catctcgatg aagtcacggc catcgtcact gcctggacgt cgcagttaac
     1501 caaagaggaa gccacggaac aggcgcgacg gttccgcatt ccctgctcgc cggtgcgcga
     1561 cgtctcggag gtgatgcacg atccgcacat gcacgctcgt ggcatgctcg agtgggtgga
     1621 acatcccgac ctgggccggg tcgtgctgcc gacaacgccg attcggctgc atgacacggc
     1681 cgttgccgcg accatgccga gcccgcggct cggtcagaac aacgacgatg tctatggcgg
     1741 ctggctgggg ttggccgcgg acgaaatcgt ggcgttgcgc gcggagggcg tgatctagac
     1801 ccctgtgggc ggcgccggat gcgcgatacg ctcggccggc aggggctggt gcagcagaag
     1861 aggcgagggc aggcatcgtc tgcggggcag ggggcctgcg cgctgaaatg cgacacggca
     1921 ggtgtttccg aaaagatttg cgcgacaagc gcccttttaa gggcaggtat tcagtccact
     1981 atgctggcgt gagcttgtac atatggtgta ggctcgtaag taagccatac tcctggagac
     2041 atgccatgaa ggctagagtc atgcctgatg gtcggcttag ccttcccgca gggcttcgca
     2101 aaaagcacgg cctgatcaag ggcggagagg tcatcgtcga agataccggc gatgcgatca
     2161 tgctacgcac attcgaccaa gtggtcgcac gagcccaggc actcagccga aagctggttg
     2221 aaggcaaggc cggctcctcg gttgacgact ttctgtcaga tcgggcgcgt gaggcagaag
     2281 ctgagtgacg gatgtcgtgc tcgacgcttc ggcgctactc gccctgcttc tggacgagcc
     2341 cggcggcgac aaggtgaagg cgatgctgga tggcgcgcta ttgggagcgg tgaatttggc
     2401 cgagattgtc agccactacg ccaagctcgg ggccgctcga tctgacatcg aggcgttgct
     2461 acagccactg ccggtccgcg tggtgccgat agacgagcgt ctgtcctacg aagcggggat
     2521 gctccggccg gtgacgctca aggcggggct gtcgctcggc gatcgctact gcctggcact
     2581 ggcgaagcgg gaaggcgtcc cggctgtgac agcggagcgt cgctggcccg acatcgccgc
     2641 cgccgcgggc gttacggtgg atctgatccg ttagccctaa aggccgaaga acccttggtc
     2701 cgcccgatat gcgacgcgcc gtcggtcgct aaatcacagc aattttccac aaacattggt
     2761 tcgtctacac ttccccccca cctacgcgcg ataagcgcgg ttatcgcgcc caatcgaatt
     2821 aatcggctgg ctatccgaag acagggcagc taaagcgaca ttcatcaatg ctgcggtgga
     2881 atgtcgcgat acggccgtcc tgccgggtgt gacggcgttc acagacggac cggctatacc
     2941 tcggtatgct ccccatcgaa agcaccgggg gcgcaagaaa cgcatgatgt caattgctta
     3001 gcagcactcg gggtaatacc ggcaaaatct cgggcgcagc cgcatttttc gcttggtgcg
     3061 acacggcggt gtacgattca agctatgcgt gagcacgtcc cccaacctct ctgcgttaag
     3121 ccggccgaag cttgaagcgc tcacggtcga ggtcatggat gagaacgccg cactgaagca
     3181 gatggtcaac cagcaacgcg aggagatcgc ctggctgaaa ggactgaagg gccgcccgga
     3241 catcaaacca agcggtatgg agaaaggcac cgaaccggcc aaacccagca ggcaagacaa
     3301 gcgccccacg cgcggcaagg tccgtccccg ggtcaatgtc gaggatcgga tcatcaaggc
     3361 catcgtcccg cccggatcac gcttcaaggg gtacgagacc tatcaggtcc aggacctggt
     3421 gctggcggtg cacgcggtcc gctactgccg cgagcgttgg gtcacgccag agggcaagac
     3481 gatcattgca ccgctgcccg agggcacccg gggacacttc ggtcccgacc tgcgccgctt
     3541 tgtgttgatg cagtatcacc agggtcagac gacgttgccc cgtcttgcga cgctgttgca
     3601 gtcgctcggc gtggcgatct ccgagcgcga gatccaacgg ttgctgaccg aaggcctgga
     3661 gcaattcctt gtcgaaaacc gcgacgtgct gcgcaccggc ctgcaaacat cgtcctggat
     3721 atcggtggat gacaccggcg ctcgacatca agccaagaac ggctactgca cgcagatcgg
     3781 caatcagcat ttcacctggt tcggcacaag gtcatcgaag agccggctga acttcctcga
     3841 tctgctgcgc gccggtcact ccgacttcat gttgaacgac gcagcattcg attacatgcg
     3901 cagccgcgct ctgcctgcca gcctgatcgc ccgcctggcc gcagcggcgg acaccatctt
     3961 cgccgatcag gccgcctggt cggcgcatct cgcccggctg ggcttcaccg gactgactac
     4021 cacacccgac ccggtgcaga tcgccaccga aggcgccatc tggggcagcg tccacgcaca
     4081 cgactttctg cgcaatgccg tggtgctgag tgacgatgcc ggacagttcg cggtcggccg
     4141 gcacgccctg tgctgggtgc atgcggaacg gctggtgcat aagctggaca ccttcaccga
     4201 tcagcaccgt gacgcgcagc aaacggtgcg caagctaatc tgggacttct acgccgatct
     4261 caaggcatac caagctaatc ctgacaaacg ctgccgtcct gcgctacggg cgcggttcga
     4321 ccgcatcttc cgccgccgca ccggctttgt caccctcgac cgcctgctgg cgcgactgca
     4381 cgctaacaag gccgagctgc tgatggtgct ggagcgaccc gagattccgc tgcataccaa
     4441 tggctcagag aacgatatcc gctgccaggc cacaaggcgg aaagtaagcg ccgggacacg
     4501 cagcgatctc gggcgggact gccgcaatgc cttcctgggc ctcggcaaga cctgcgccaa
     4561 actgggcgtg gcggtctggg actttctcgg caaccggctg ggcgtccccg gacaccttct
     4621 cgttccgtca ctgccggagc ttattcgttg ccgtggtcag ccggcctgat cgggccggct
     4681 gcccggagtt gtgccggtat tacgatgtgt gcatccccta gccccgccat gacggtcgcg
     4741 gcagtcgatc gccttgttca ccattccatc atcctggaga tgaacaccaa gagctaccgg
     4801 cgaaaggtcg ccgaagccaa cagcccgaga gcgccggtc
//
LOCUS       DASWQN010000164         4197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1073162, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000164 DASWQN010000000
VERSION     DASWQN010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073162"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1316)
                     /gene="paaK"
                     /locus_tag="VGC82_02730"
     CDS             complement(18..1316)
                     /gene="paaK"
                     /locus_tag="VGC82_02730"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HEY0422216.1"
                     /translation="MSSVEPIETASVDELRAIQLVRMRQALQRAYERVPHTRSRFDAM
                     GVHPDDLLRLEDIAKFPLTAKDDLRQNYPFGMFAEPMEKIVRIHASSGTTGKPTVVGY
                     TRNDIETWSMLMARSLRAAGGRPSDIIHVAYGYGLFTGGLGAHYGGEYLGAAVIPVGG
                     GFTERQVQIIRDFKPDIIMVTPSYMLAIADEFERQGVDPGGCSLRLGIFGAEPWGEGM
                     RTELERRMGLQAFDVYGLSEVMGPGVAQECLDSKGALTIWEDHFYPEIIDPVSGVPVA
                     EGAFGELVLTTLTKEGMPVIRYRTRDLTRLLPPATRAMRRIDRISGRSDDMLIIRGVN
                     VFPSNIEAVLAKEQRLAPHYLLELRRPGSLDELDVVVETRTALGGKLSGAELAELERR
                     TEHSIKALVGITTKVRVVEPGTLERSQGKAKRVLDLRPKD"
     gene            complement(1380..2447)
                     /locus_tag="VGC82_02735"
     CDS             complement(1380..2447)
                     /locus_tag="VGC82_02735"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0422217.1"
                     /translation="MPQPNNRYGLTAARSHSRPGRESRPKSLTVDIHSHVLVPDAASY
                     ARPHQAPDPRAKVYTEETRTLTRMQDEDRTPNLVDLSLRMRDFDAMGVDAQVISPAPP
                     QCYYGVPAEVGIKAARMVNDGVAEIAAKMPDRIPAAMGSVPLQAGGEAAANELEYAIK
                     TLGFKGVEVLAHVGELELSDPSFEPFWAKAEALGAVVFIHPNSFTEPRRFARFYFSNV
                     IGNPLDTTMALHHLIFDGVLERYPNLKIIASHGGGYLPAYSGRIDHAWGARSDAHGAL
                     PKPPSFYLKKIFLDTIVFTPDQLEALVKLFGVEKILLGTDYPYDMGEYDPIGHIASVA
                     SFSEADRAAIAGGNAKTLFGF"
     gene            2599..3471
                     /locus_tag="VGC82_02740"
     CDS             2599..3471
                     /locus_tag="VGC82_02740"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0422218.1"
                     /translation="MAKPPASRLPVILSESATEILSDWLARLNTRPDPRLAAAELETQ
                     CDGIVRRLAQACRDGNVTDIDAPSFAALRDILAELSRTRALAGYSPTETAIFILSLKE
                     PLLARLRSVLAAEPDRLSDDTATTGELVDKLGLFMMETFQRAREEVIIRQQMEISELS
                     TPVVRLWQGILALPLIGTLDSARTQVVMENLLDSIVRYEADIAIIDITGVPTVDTLVA
                     QHLLKTVAAARLMGADCIISGIRPQIAQTMVHLGVELNVISKSSLADAFALALRRLGK
                     TVVNQRRAAPAEPV"
     gene            3475..3873
                     /locus_tag="VGC82_02745"
     CDS             3475..3873
                     /locus_tag="VGC82_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0422219.1"
                     /translation="MDRIPILKLGEALLVTIQVDMHDQLALALEEDLTERIVEFGAKG
                     VLIDISALEIVDSFIGRTLANIAMIARILDADTVVVGMRPAVAITLVELGLSLPGVRT
                     ALNAERGMAMIKARIDKAEMTRADEDEGGS"
     gene            3863..>4197
                     /locus_tag="VGC82_02750"
     CDS             3863..>4197
                     /locus_tag="VGC82_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0422220.1"
                     /translation="MARSTVPIQTGEDVVRVRQEVRRFAIQAGLGLVDQTKVVTAASE
                     LARNTLDYGKGGRATMDVVQNGARQGVRIVFEDDGPGIADIDLALKDGYTSGKGMGLG
                     LGGARRLCS"
BASE COUNT          800 a         1384 c         1234 g          779 t
ORIGIN      
        1 cttgcaagtc catcgcatca atcctttggc cgcaggtcca ggacgcgttt cgccttgccc
       61 tgcgagcgct cgagcgttcc cggctcgacc acgcgaacct tggtcgtgat gcccaccaac
      121 gcctttatcg aatgctccgt ccggcgctcc agttcggcca gttcggcacc gcttagtttg
      181 ccgcccaacg cggttcgcgt ctcgaccacc acatcgagtt cgtccaggct tccgggccgg
      241 cgcagttcca gcaagtaatg cggcgccagc cgttgttctt tcgcaagtac cgcctcgatg
      301 ttcgacggaa acacgttgac cccgcgaatg atcagcatgt cgtcgctgcg gccgctgatc
      361 cggtcgatcc gccgcatcgc acgcgtcgcc ggcggcagca gccgagtcag gtcgcgcgtg
      421 cggtaacgga tgaccggcat cccttccttg gtcagcgtcg tcaggaccag ttcgccgaac
      481 gctccctcgg caacaggcac gcccgagacc ggatcgatga tctcgggata aaaatggtcc
      541 tcccagatcg tcagcgcgcc tttggaatcc aggcattcct gcgcgacgcc gggccccatg
      601 acctccgaca gcccgtaaac gtcgaacgcc tgcaacccca tgcgccgttc cagttccgtt
      661 cgcatgcctt cgccccacgg ttcggcaccg aaaatgccca gccgaagact gcatccaccg
      721 gggtcaacac cttgccgttc gaattcgtcg gcgatcgcca gcatgtagga cggcgtcacc
      781 atgatgatat ccggcttgaa gtcgcggatg atctgcacct gccgttcggt gaaccctcct
      841 cccaccggaa tgaccgccgc gcccagatat tcgccgccgt aatgcgcacc cagcccgccg
      901 gtgaacaagc catacccata ggcgacatgg atgatatccg acggtcgtcc gccggctgcc
      961 cgcagcgacc gggccatcag catcgaccag gtctcgatgt cgttcctggt atagccgacg
     1021 acggtgggct tgccggtggt gccgctggac gcatgaatgc ggacgatctt ctccatcggt
     1081 tcggcgaaca tcccgaacgg atagttctgc cgcaggtcgt ccttcgccgt cagaggaaat
     1141 ttcgcgatat cctcaagccg cagaagatcg tccggatgca cccccatcgc gtcgaagcgg
     1201 gatctggtat gcgggacccg ttcataagcg cgctgcaacg cctgccgcat ccgaacaagc
     1261 tgtattgccc gcagctcatc gacgctggcg gtctcgattg gttcaaccga gctcatgact
     1321 ccctacccat acattccgtc tcccgccgcg tccaaggcag catccgcggc ggcgctgccc
     1381 taaaacccga acagtgtctt ggcattgccg ccggcgatcg ccgcgcggtc cgcctcgctg
     1441 aacgatgcca ccgacgcgat gtggccgatc ggatcgtact cccccatatc gtacggataa
     1501 tcggttccaa gcagtatctt ctccacgcca aacagtttga ccagggcctc taactgatcc
     1561 ggtgtgaaaa caatcgtatc cagaaaaatc ttcttcaagt aaaagctcgg cggcttcggc
     1621 aatgcgccat gcgcatcgct gcgggcgccc caggcatggt cgatccgtcc ggaataggcc
     1681 ggcaggtacc cgccaccatg cgaggcgatg atcttcaggt tcggataccg ctccagcacg
     1741 ccgtcgaaga tcaggtgatg cagtgccatc gtcgtatcca gcgggttgcc gataacattg
     1801 ctgaagtaaa acctggcgaa acgcctgggt tcggtgaagc tgttgggatg aatgaacacc
     1861 accgcgccca acgcctcggc cttggcccag aagggctcga acgacggatc cgaaagctcc
     1921 agctcgccga catgcgccag tacctcgacg cctttgaagc ccagcgtctt gatcgcgtat
     1981 tccagttcat tggccgccgc ttcaccgccg gcctgcagtg gcaccgaacc catggcggcg
     2041 ggaatccggt caggcatttt cgccgcgatc tcggcgacgc catcattgac catgcgagcc
     2101 gccttgatgc ccacctcggc cggcacgcca tagtaacatt gcggcggggc gggactgatc
     2161 acctgcgcat ccacccccat cgcatcgaag tcgcgcatcc gcagcgaaag atccaccaga
     2221 ttgggcgtcc ggtcttcgtc ctgcatgcgg gtcagggtgc gggtttcctc ggtgtacacc
     2281 ttcgcccgcg gatcaggtgc ctgatgcggc ctggcataac tggccgcatc aggcaccagc
     2341 acatgcgaat ggatatccac cgtcaggctt ttgggcctgg actcccggcc cgggcggcta
     2401 tgagaccgag cggcggtaag gccgtagcga ttgttgggtt gaggcactga tggttcctcc
     2461 gcaaacgtcc gataaagcat caaagccgcg ccaagccgcc aaatcaatca cgcccgagtg
     2521 agatcgtgcg cgttactctg gctattttcg ttcgaccccg tagctttgtg cgcctcagat
     2581 acaggatcga gcacgatcat ggccaaaccg cctgcatctc gccttcccgt catactttcg
     2641 gaatcggcga cggaaatcct ctctgactgg ctcgccagac tgaacacgag gccggatccc
     2701 cgcctggcgg ccgctgaact ggaaacccag tgcgacggca tcgtcaggag actggcgcag
     2761 gcttgccgcg atggcaatgt cacggacatc gacgcgccat ccttcgccgc gttgcgggat
     2821 attctcgcgg agctatcgcg aacccgagcc ctggccggat actcgccaac cgaaaccgcg
     2881 atcttcatcc tgtcgctgaa ggaaccattg ctggcccgcc tgcgcagcgt gctggccgcc
     2941 gaacctgacc ggctgtccga tgacaccgcg acgaccggcg aactggtcga caagctcggc
     3001 ctgttcatga tggagacttt ccagcgggca agggaagaag tcatcatccg ccagcagatg
     3061 gagatctcgg agctatcgac gccggtggtc aggctgtggc agggcatcct cgcattgcca
     3121 ctgatcggca cgctggattc cgcccgcacg caggttgtga tggagaacct gctggacagc
     3181 atcgttcgat acgaggccga tatcgcaatc atcgatatca ccggtgtgcc gaccgtcgat
     3241 accctcgtcg cccagcatct gctcaagacc gtcgctgccg ctcggcttat gggtgcggac
     3301 tgcatcatca gcggcatccg gccgcagatc gcccaaacca tggtgcatct cggcgtcgaa
     3361 ctcaacgtga tctccaagtc cagcctcgca gatgcgttcg ccttggcgct ccgccgcctg
     3421 ggcaagacgg tcgtcaacca gcgccgcgcc gcgccggccg aaccggtata acagatggac
     3481 cggatcccta tcctgaagct gggggaggcg ctgctcgtca ccatccaggt tgacatgcac
     3541 gaccaactgg cgctggcact ggaagaagac ctgaccgaac ggatcgtcga gtttggcgcc
     3601 aagggggtgc tgatcgacat ctcggcgctt gagatcgtgg acagtttcat cggccgcacc
     3661 ctggccaaca ttgccatgat cgcaagaatt ctcgatgccg acacggtggt cgtcggcatg
     3721 cgccctgctg tcgccatcac cttggtggag cttggcctgt cgctcccggg tgtgcgcacg
     3781 gcgctgaacg cggagcgtgg catggccatg atcaaggccc ggatcgacaa ggcggaaatg
     3841 actcgagcgg acgaggacga gggtggctcg tagcacggtg cccatccaaa ccggcgagga
     3901 tgtcgtgcgg gttcgccagg aggtacgccg cttcgccatc caggccggac tcggcctggt
     3961 tgaccagact aaggtcgtaa ccgccgccag cgagcttgct cgcaatactc tcgactacgg
     4021 caagggcggc cgtgcgacga tggatgttgt acaaaacggc gcccgacaag gcgtccgcat
     4081 cgtgttcgag gatgacggcc cgggtatcgc cgatatcgat ctggcgttga aggatgggta
     4141 caccagcggc aaaggcatgg gccttggtct aggcggcgcc aggcgcctgt gcagcga
//
LOCUS       DASWQN010000165         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1205492, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000165 DASWQN010000000
VERSION     DASWQN010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205492"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VGC82_02755"
     CDS             <1..678
                     /locus_tag="VGC82_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422221.1"
                     /translation="DYGSNFFNADVLFSNANEPAANSSGGSTEFYGVYRGQFSPDKIF
                     GLNTAIGPFSAINFEVGGDAETENSAFGPNKKLLVAGPNFHVNMQAGFFDIGVHISKE
                     WNHNGFSSCPKSCGKGGPIDFDPAPEFEFVWLYPLGFTGLPLDFRGFMNIVLPKGTDG
                     FGNHTYTEILARPQLQLDVGKMVFNKSHKPDVYLAVEFWHHKFGNSSAVSGSDQIAPT
                     IGIDYHF"
     gene            761..1960
                     /locus_tag="VGC82_02760"
     CDS             761..1960
                     /locus_tag="VGC82_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY0422222.1"
                     /translation="MIDTLLDWISLLVRWTHVIAGIAWIGSSFYFIALDAGLQANPRL
                     DPRVKGEAWQVHGGGFYNIQKFTVAPDFMPEHLTWFKWEAYGTWIFGFALLVLTYYLN
                     PTVYLIDPAVMNIDAADAVPAAAASLVIGWFVYDLLCRSWFGRETARLAISGFVLLVG
                     VTYAYTHVFSGRGAFIQVGALVGTIMAANVLIIIIPGQRKVVASLLKGEEPNPIYGYQ
                     AKQRSIHNNYLTLPVVFTMLSNHYAFTYNSRWSWLILACIFVASFLVRHWFNTMHTGA
                     KPDWRLWPAAAVPIVCAIVLGMIGQPSLPLADAGAAPVTFADVRQIVDQRCHICHAAK
                     PRFPGFAEAPKGVMFDTPAQIKRQAPQIMAQAVKTRTMPLGNVTEITEAERSTLGSWI
                     AAGSKLN"
BASE COUNT          409 a          662 c          634 g          465 t
ORIGIN      
        1 gactatggct cgaacttttt taacgctgac gtgctgttct cgaacgccaa cgagcccgcg
       61 gcgaactcat cgggcggatc gaccgagttc tacggcgttt accggggcca gttcagcccc
      121 gacaagattt ttggcctcaa cacggcgatc gggccatttt cggcgatcaa ctttgaagtc
      181 ggcggtgacg ccgaaaccga aaattcggcg ttcggaccaa acaagaaact gctggtggca
      241 ggcccgaatt ttcatgtgaa catgcaggcc gggtttttcg acatcggcgt tcatatctcc
      301 aaggaatgga accacaacgg cttctcaagc tgccctaaat cgtgcggtaa gggcgggccg
      361 atcgatttcg atcccgcgcc cgagttcgaa ttcgtttggc tttatccgtt gggcttcacg
      421 ggcttgccgc tggacttccg gggattcatg aacatagtcc tgccaaaggg caccgacggc
      481 ttcggcaacc acacctacac cgagattctt gctcggccgc agcttcaact ggatgttggc
      541 aagatggtgt tcaacaaatc gcataagcct gacgtctatc tcgcggtgga attctggcac
      601 cacaagttcg gcaattcctc agcggtatcg ggttcggatc aaatcgctcc gacgattggt
      661 atcgactatc acttctgata ggctggtccg cggcggtctt cggatcgccg cgaaccatga
      721 gcccaacgga tcatgagcct gacggaccgg gaacccgccg atgattgaca cgctgctcga
      781 ctggatcagc ctgctggtcc gctggacgca tgtgatcgcc ggcatcgcct ggatcggatc
      841 atcgttctat ttcatcgcgc tcgacgctgg tttgcaggcc aatccgcggc ttgatccgcg
      901 ggtcaagggc gaagcctggc aggttcacgg cggcggtttc tataacatcc agaaattcac
      961 ggtcgcgccg gatttcatgc ccgagcacct gacctggttc aagtgggagg catacggcac
     1021 ctggatcttc ggcttcgcgc tgctggtgct gacctactac ctcaacccaa ccgtttacct
     1081 catcgacccc gcggtgatga acatcgacgc cgcggacgcc gtgcccgcgg cagccgcatc
     1141 cctggtgatc ggctggtttg tctatgactt gctgtgtcgc tcctggttcg gtcgcgaaac
     1201 tgcacgcctg gccatctccg gcttcgttct gctggttggg gtgacgtatg cctatacgca
     1261 tgtgttcagc ggtcgcggcg ctttcatcca ggtcggcgcg ctggtcggca cgatcatggc
     1321 ggccaacgtc ctgatcatca tcattcccgg acagcgcaag gtggtggcgt cgctgctgaa
     1381 gggcgaggaa cctaatccga tctatggcta ccaggcaaag cagcgctcca tccacaacaa
     1441 ctacctgacg ttgccggtcg tgttcacgat gctgagcaac cactatgcct tcacttacaa
     1501 ttcgcgctgg agctggctga tcctggcgtg catcttcgtc gccagcttcc tcgtaaggca
     1561 ctggttcaac accatgcaca cgggtgcgaa gcccgattgg cgtctgtggc cggccgccgc
     1621 cgtgccgatc gtctgcgcga tcgtcctggg gatgatcggt cagccatcgc tgccgctggc
     1681 ggacgccggc gcggcaccgg tcaccttcgc cgacgtcagg cagatcgtcg atcagcgctg
     1741 ccacatctgt cacgccgcca agccgcggtt cccggggttc gccgaggctc cgaagggggt
     1801 gatgttcgac acgccggccc agatcaagcg ccaagcgccg caaatcatgg cccaggccgt
     1861 gaagacccgc accatgccgc tgggcaacgt gaccgagatc accgaggcgg agcgatcgac
     1921 cctggggtcg tggatcgccg ccggatcgaa gctgaactag ccttgccgtg tccggaccac
     1981 cctcgatgca cggtgccggt gcgacaacgc catgacacac gtatgacgaa tgccttctgc
     2041 gaggtcatat atatggaaca aacggtcgca ttttgatcaa agttgatgca acaggtagtc
     2101 cgggagtctg tgcggcgttt cttcgttgac cggtttgcga caatcggcct tagtttcatc
     2161 ctgcgtgacc
//
LOCUS       DASWQN010000166         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_545970, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000166 DASWQN010000000
VERSION     DASWQN010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545970"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..592)
                     /locus_tag="VGC82_02765"
     CDS             complement(23..592)
                     /locus_tag="VGC82_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0422223.1"
                     /translation="MISLRKARVADAIAIGAVHVAAWRSTYPGILPDGFLAKMSVSRQ
                     AAFYDAAIRGGTGVTVATASGADVPSGSGPRIIGFTTAGRARNVDFAGKRLAEGEVET
                     LYVLDDWRERGVGRRLMRAAGTHLVDIGCKSAFLWVLRDNPSRWFYQRLGGRPAAEQT
                     IQIAGQTVAQTAFVWNPIELLLTASPQAS"
     gene            complement(589..1878)
                     /locus_tag="VGC82_02770"
     CDS             complement(589..1878)
                     /locus_tag="VGC82_02770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY0422224.1"
                     /translation="MTPAARIAAAIDLLEVIEGAPKRPADAVANDFFRSRRYIGSGDR
                     RAVSDRVWIVLRARRRLGWWLGGAAQSSRLLVAASLLLEGWAKAGVAQAFSGGQFAPM
                     PLSGAENAVLSRIAGHTLNHPTMPEAVRLEVPDWLLPKLVARFGNSLAAEMAALSEPA
                     ALDMRVNLLKGDREQARAALAAEGWEAELTKLSPWGLRIEGRRPVTSGPTFQSGLIEI
                     QDEGSQLIAAMVGATPGMRVVDWCAGAGGKTLALAGAMANRGQIVACDVSAPRLEGAV
                     RRLRRAGAHNVERHLVEPGDKWLKRRTGTFDRVLVDAPCTGTGTWRRNPDARLRLQET
                     DLVELLAKQAAILDTAQSLVRKGGRLVYATCSLLEEENEAQVSAFLLRHPAFSVVPLA
                     RAWPLDQPPPNSGEFLSLTPARHGTDGFFAAVLELTT"
BASE COUNT          444 a          814 c          744 g          380 t
ORIGIN      
        1 gctggaccac caaacccccg gattaagacg cctgcggcga agccgtcagc agcagctcga
       61 tcggattcca cacaaatgcc gtctgggcga cggtctgtcc ggcgatctga atcgtctgtt
      121 cagccgccgg tctgccgcca agccgctgat agaaccaccg gctcgggttg tcgcgcagca
      181 cccacaggaa cgccgatttg cagccgatat ccaccagatg agttcccgcg gctcgcatca
      241 ggcggcgacc gacaccgcgc tcccgccagt cgtccagcac gtaaagcgtc tcgacttcac
      301 cctcagccaa gcgtttgccg gcaaaatcga cgttcctggc ccgtccggcg gtggtaaagc
      361 cgatgatccg cggaccgcta cccgacggca catccgcccc cgaggcagtc gcaaccgtga
      421 cgcccgtgcc accgcggatc gcggcatcat agaaggccgc ctgccgggag accgacatct
      481 tggcgagaaa cccatcgggc aagatgcccg gatatgtgct gcgccacgcc gcaacatgga
      541 ccgcaccgat cgcgatggca tcggccaccc gagctttacg aagggaaatc aagtcgttag
      601 ctccaaaacg gcggcgaaga agccatcggt gccgtgccgg gcgggcgtta acgacaagaa
      661 ctcgccgcta ttcggcggcg gttgatccag cggccatgcc cgcgccagcg ggacaacgga
      721 aaacgccgga tgacggagca gaaaggcgga cacctgtgcc tcgttctcct cttccagaag
      781 ggagcaggta gcgtacacca aacgccctcc tttgcgaacc aaactctgtg ccgtgtcgag
      841 aatggcggcc tgcttggcca gcaactcgac caaatcggtc tcctgtaacc gcagtcgggc
      901 gtcggggttg cggcgccatg tgccggtgcc ggtgcatggc gcatcgacca gcacccggtc
      961 gaacgtgccg gttcggcgtt tcagccattt gtcgccgggt tccaccaggt ggcgctcgac
     1021 attatgggcg ccggcgcggc gcaggcggcg gacggctccc tccaggcggg gtgcggagac
     1081 atcgcaagcg acgatctgcc cgcgatttgc catcgccccg gccaaagcca gggttttgcc
     1141 gccggcgccg gcgcaccagt ccaccacccg catgccgggc gtggcgccga ccatggcggc
     1201 aataagctgg ctgccttcgt cctggatctc gatcagtccg gactgaaacg ttggcccgct
     1261 ggtaaccggg cggcggccct cgatgcgaag tccccagggc gagagtttgg ttaattcagc
     1321 ctcccaaccc tcggcggcca gggcggcgcg ggcctgctcg cggtcgccct tcagcaggtt
     1381 gacgcgcata tcgagcgcgg ccggttcgga cagggccgcc atttccgcgg ccaggctatt
     1441 gccgaaacgg gccacgagtt tgggcagcag ccagtcgggc acttccaggc gcaccgcttc
     1501 cggcatggtg ggatggttca gcgtgtgccc ggcgatccgc gacaggacgg cgttctccgc
     1561 cccggacagc ggcatgggtg cgaactggcc gcccgaaaat gcttgcgcca cgccggcttt
     1621 cgcccagccc tccagcagca gagatgccgc gaccagcagg cgcgacgact gtgcggcccc
     1681 gccgagccac cagcccaaac ggcggcgggc tcgcagtacg atccagaccc ggtccgatac
     1741 ggcgcggcgg tctcccgaac caatataacg gcgcgagcgg aagaaatcgt tggcgacggc
     1801 atcggccggt cgtttcgggg cgccttcgat gacctccaga agatcgatcg cggcggcgat
     1861 gcgtgcggcg ggagtcacgc cggcaggtcg ggcggttccg acagattagg cactttatag
     1921 tcaccttttg accaaggaaa gaagaggatc ggatacaccg gcgcagctcg tttgaccatt
     1981 tgaattcata tcccaaattg cgatgacctc aactccccag caattccagc tttgcttccg
     2041 tggcgctacc gaggttcgaa cggagtttga cgatcggacg ccgtccagtg gatcggcccc
     2101 gcgcgtgccg atccgacgat cgcttcaacg ccgagtgcgc caaggacgct gagatcgcgt
     2161 tgcccgtgcg cctcaagctg cgccacttgt gcaccctcac acaagccgcg aagcggccca
     2221 agccaaaggt cccgaccgtc aacagcgacg cagactttcc tgctccgcgc cctccaggat
     2281 ctccgtgcgc tccatgtcga aagccttgag caacctccgc acaggcctca aacgtcgccc
     2341 tccgggagta tttccggtct gcgagatcag gctgcaggcg ct
//
LOCUS       DASWQN010000167         3242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_677837, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000167 DASWQN010000000
VERSION     DASWQN010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677837"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..>3242
                     /locus_tag="VGC82_02775"
     CDS             216..>3242
                     /locus_tag="VGC82_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0422225.1"
                     /translation="MELVKYALKFRITFYVMAVLILLSGVGGYIIMPKDVLPEVNIPV
                     VTIVWTYTGLDTPEMEKRVTTYTEFALSNNVNGIRDMESTTLQGVTVTRIYFQPDVSI
                     DLALSQVTASTNSIRAALPPGIQPPVVMRFSASTVPVIQLALSSQQENERQLYDYGQY
                     RIRQTLTTVPGSTLPSPYGGQPRQIMVDLDLHALQAYGLTPADVTSAMTAQNITVPSG
                     LAKFGAMQYVIRLNSTPEAIATLNEVPIKVVNGSPILVRDVANVRDGGPPQQNIVRQD
                     GMRGVLLTVLKNGNASTLSVVNTVKSLLPGIREAAPKDLKITPLFDQSVFVSGAIDDV
                     IREGAIAAGLTGMMILLFLGSWRSTLIVLISIPLSILTSLAVLGALGETVNIMTLGGL
                     ALAVGILVDDVTVAIENTYRLLDEGRDFRQAVVEGAAGIAKPALISTLSICAAFVSVM
                     FLTDAPKYLFTPQALAVVLAMLASYVLSRTLVPVLIDVLVKREHEKRGDADKKPPGFF
                     GRVHAGFERRFKTFQNAYVGLLHVVILRRWITLGAAGTTIAVAAVLFMFVGTDYFPQI
                     DAGQMTLHVRAPPGMRIEQAEKLFQQVEDTVRQVVPPKEIGTIIDNIGLPASNYNFAF
                     GNGTFVAYNDGQVLVTLAPGHGPTLNYQKRLRALLQQRFPEAVFYFEAADMITQILDF
                     GVPSQIDVQVSGRQAGKDLQVAQAIANKLRMVRGAVDVHIQQITNAPEFFVKVDRQRA
                     AELGLTEQQVASDLNVSLSGSFQVSPNFWTDPKTGIPCQVWVQTPEYRNASIDALANT
                     PLLISAGSGTGPTALQLLSNVATMQRGSEQTVANHVNTQPTYDVYAAVQNRDLGSVES
                     EVNQIVAEQQKQLQPPDKIAVRGQIQSKDQAFLHLGIGLAVALVAVYLLMVVNFQTWG
                     DPFVVLAALPLAFCGIVASLFITQTSFSIPSLMGAIMSVGVASANSILLVTFAREHRE
                     RTGCGAMEAAIAAGGTRLRPVLMTAGAMFVGLLPMAI"
BASE COUNT          605 a          987 c         1015 g          635 t
ORIGIN      
        1 acactctttg tgaccacggc agtctttgcg gccacgacag tctttgtagt cataagcata
       61 gccacgtcgg tcttcatagt cacgggcacg gctatgacac tttttgtatt caccggcgca
      121 gctatgacag tcgttgcagt cacatgcaca ggcagagcgg cggaacggga gaccgtgccc
      181 gaagcagcta ttttgaagcg gttgcctagc gcgacatgga actcgtcaag tatgcgctga
      241 agttccggat cacgttctac gtgatggcgg tcctgatcct gctgtccggc gtcggcggct
      301 atatcatcat gccaaaggac gtgcttccgg aagtcaacat cccggtcgtt acgattgtct
      361 ggacatacac tggcctcgac acgccggaaa tggaaaagcg cgtcaccacc tacacggagt
      421 tcgcgctcag caacaacgtc aacggcatcc gcgacatgga gagcacgacg ctgcaaggcg
      481 tcaccgtaac ccggatctat ttccagccgg atgtcagcat cgacctggct ctgtcgcaag
      541 tgacggcgtc caccaactcg atccgagcgg cgttgccgcc tggcatccag ccgccggtgg
      601 tgatgcggtt ttcggcatcc accgttccag tgatccagtt ggcgctcagt tcgcagcagg
      661 agaacgagcg gcagctctat gactatggac agtatagaat ccggcagact ttgacgacgg
      721 ttccgggttc gacgctgcct tcgccgtatg gcggccagcc gcgtcagatc atggtggacc
      781 tcgaccttca cgcgctgcag gcttatgggc tgacgcccgc ggatgtcacc tccgcgatga
      841 ccgcacagaa cattaccgtg ccatcgggcc tggcgaagtt cggtgccatg caatacgtca
      901 tccggctgaa ttccacgcct gaagcgatcg ccactttgaa cgaggtgccg atcaaggtgg
      961 tgaacggctc accgatcctg gtgcgggatg ttgccaatgt ccgtgacggc ggcccgccgc
     1021 agcagaatat cgtgcgccag gacggaatgc gcggcgtgtt gctgacggtg ctgaagaacg
     1081 gcaacgcctc gacgctttcg gtggtcaaca cggtgaaatc actgctgccg ggcatccgag
     1141 aggcggcacc gaaagatctg aagatcacgc cgctgttcga tcagtcggtg ttcgtttccg
     1201 gcgccatcga cgacgtgatc cgggagggcg cgatcgccgc cggactgacc ggaatgatga
     1261 tcctgctgtt cctcgggtca tggcgctcga ccttgatcgt gctgatctcc attccgctgt
     1321 cgatcctgac ctcgctcgcg gtgctggggg cgctggggga gaccgtcaac atcatgaccc
     1381 ttggcggtct ggcgctggcg gtgggtattc tggtggacga cgtgactgtc gcgatcgaga
     1441 acacctatcg gctgctggat gagggccgcg acttcaggca ggccgtggtc gaaggcgcgg
     1501 ccgggatcgc caagccggcg ctgatctcga cgctgtcgat ctgcgctgcc ttcgtctccg
     1561 tcatgttcct gaccgacgcg ccgaaatacc tgttcacgcc gcaggccctg gccgtggtgt
     1621 tggcgatgct cgcttcctac gtgctgtcgc ggaccctggt ccctgtgctg atcgacgttt
     1681 tggtgaaacg cgaacacgaa aagcgcgggg atgccgacaa aaagccgccc ggattcttcg
     1741 gccgcgttca tgcggggttc gaacggcgat tcaagacatt ccagaatgcc tatgtcgggt
     1801 tgctgcacgt cgtgatcctg cgccgctgga taacgcttgg cgccgctgga acgaccatcg
     1861 cggtcgccgc cgtcctgttc atgttcgtcg gcaccgacta cttcccgcag atcgatgccg
     1921 ggcaaatgac gctgcatgtg cgggcaccgc cggggatgcg catcgagcag gcggaaaagc
     1981 tgttccagca ggtcgaggac actgtccgcc aggtggtgcc accgaaagaa atcggcacca
     2041 tcatcgacaa tatcggcctg ccggcttcca actataactt cgcgttcggc aatggcacgt
     2101 tcgttgccta caacgacggc caggtgctgg tcacgctggc gcccggccat ggcccgaccc
     2161 tgaactatca gaaacggctg cgcgctttgc tgcagcagcg ttttccggaa gcggtgttct
     2221 acttcgaggc cgcggacatg atcacgcaga tcctggattt cggtgtgccg tcgcagatcg
     2281 acgtgcaggt cagcggccgg caggcgggca aggaccttca ggtcgcacag gccatcgcca
     2341 acaagctgcg gatggtacgg ggggcggtgg acgttcacat ccagcagatc accaacgccc
     2401 ccgagttctt cgtcaaggtc gatcgccagc gggcggcgga gctcggtttg acggaacagc
     2461 aggtcgccag cgatctgaat gtgtccttga gcggcagctt ccaggtcagc ccaaacttct
     2521 ggaccgatcc gaaaaccggc attccgtgtc aagtttgggt gcagacgccg gaataccgga
     2581 acgcttcgat cgatgccctt gccaacacgc cgctgctgat ctcggcagga tcgggaaccg
     2641 gtcccactgc cttgcagttg ctgtccaacg tcgcaaccat gcagcgcggc agcgagcaaa
     2701 cggtggcgaa ccatgtcaac acgcagccga cctacgacgt ttatgcggcg gtgcagaacc
     2761 gcgacctggg aagtgtcgaa agcgaggtca accagatcgt cgccgaacag cagaagcagt
     2821 tgcagccgcc cgacaagatc gcggtgcgcg gccagatcca gagcaaggac caggcttttc
     2881 tgcatctcgg catcggcctg gccgtcgcac tggtggcggt ctatctgctg atggtcgtca
     2941 acttccagac ctggggcgat ccattcgtgg tcctggccgc attgcccctg gcgttctgcg
     3001 gcatcgtggc cagcctgttc atcacccaga ccagtttctc catcccgtcg ctgatgggcg
     3061 ccatcatgag cgtgggcgtt gcctcggcga actccatcct gctggtgacc tttgctcggg
     3121 agcaccggga acgaacgggg tgcggcgcga tggaagcggc cattgccgcc ggagggacca
     3181 ggctgcggcc ggtgctgatg accgccggcg cgatgttcgt gggattgctg ccgatggcga
     3241 tc
//
LOCUS       DASWQN010000168         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_414181, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000168 DASWQN010000000
VERSION     DASWQN010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414181"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..2184
                     /locus_tag="VGC82_02780"
     CDS             283..2184
                     /locus_tag="VGC82_02780"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEY0422226.1"
                     /translation="MLLPEEAAVLDAVQMPGRYQVRVDRSRDALLTDFGRATLTDRYL
                     MPGEEFQDLFARVASQYGDNQAHAQRLYDYMSKLWFMPSTPILSNGGTNRGLPISCFL
                     NEATDSLDGILGLWNENVWLASKGGGIGSYWGNLRSIGEKVGAVGKTSGVIPFIRVMD
                     SLTLAISQGSLRRGSAAVYLPVWHPEIEEFIEIRRPTGGDPNRKALNLHHGIQIPDAF
                     MRAVEADEPWSLLSPKDKSVVRSISARALWVRILTARIETGEPYLVFSDHVNNARPEH
                     HKLAGLEVKTSNLCSEITLPTGEDHLGNQRTAVCCLCSLNLENWAEWEGHPGFIEDIL
                     RFLDNVLQDFIDRAPPGMEKAKYAAMRERSVGLGVMGFHSFLQALNVPWESVVAKVWN
                     KKIFRHIRAQADAASVKLAEERGACPDALEYGVKERFSHKMSIAPTASISIIAGNSSP
                     GIEPIAANVFLQKTLSGSFSVRNRHLQKLLAEKGYDNDAVWSSITTTKGSVQHLDFLS
                     EQEKAVYKTAFELDQRWIIEHAADRAPSVCQSQSVNVFLPANVHKRDLHQIHFMAWKK
                     GVKSLYYCRSLSIARADTASDRGVAPAAFTGEMIGATLPASDKQGQLPLVAMGSGNAA
                     DYEECLACQ"
BASE COUNT          430 a          613 c          707 g          434 t
ORIGIN      
        1 ggaaaacaca ctggtcacgg accggacccc tgcccgctcg ttcatgcggt tcgacgggtt
       61 cgattgattt cgcccgtcgt tcgatagaac atacagcgaa catcattatc ggcagtgtct
      121 tgtatgatga gagacgttta acggctggcg tcatcctctg gtttctggga cggtcgtggc
      181 aatccagcca caaaatgctc tatatagtgg atttgatggg ctgctacccc atatatgggt
      241 tgtgccgaat ccatcagcct gaggagaagg gtcattcgag atatgctgtt gccagaggag
      301 gccgccgtgc ttgatgccgt tcagatgcca ggtcgctacc aggttcgggt ggaccgctcc
      361 cgcgatgcgt tgctgaccga ctttgggcga gcgaccctga ccgaccgata cctgatgccg
      421 ggcgaagagt ttcaggacct gtttgctcgc gtcgcctcac agtacggcga caaccaggcg
      481 catgcgcagc ggctgtacga ctacatgagc aagctgtggt tcatgccgtc cacgccgatc
      541 ctgtctaatg gcggcaccaa ccgtggcctc ccgatctcct gcttcctgaa cgaagcaacc
      601 gacagcctgg atggcattct gggcctgtgg aacgagaatg tctggcttgc gtccaagggc
      661 ggagggatcg gctcgtattg gggcaatttg cgctcgatcg gcgaaaaggt cggcgcggtc
      721 ggcaagacct cgggcgtcat cccgttcatc cgcgtgatgg atagcctgac cctggcgatc
      781 agccaggggt cgcttcgccg cgggtcggca gcggtttatc tcccggtctg gcatcccgag
      841 atcgaggaat tcatcgaaat ccgccgccct accggcggcg atccaaaccg caaggcgctg
      901 aacctgcatc acggcatcca gatccccgac gccttcatgc gtgcggtgga agccgatgag
      961 ccgtggtcgc tgctttcgcc gaaagacaaa tccgtggtgc ggtcgatctc ggcgcgcgcg
     1021 ctatgggtgc gcatcctgac ggcgcggatc gagaccggcg agccgtacct ggtgttctcc
     1081 gaccatgtga acaatgcgcg ccccgagcac cacaagctgg ccgggctgga ggtgaagacc
     1141 tccaatcttt gcagcgaaat caccttgccg acaggggaag atcacctcgg caatcagcgc
     1201 accgcggtgt gctgcctgtg cagcttgaac ctggagaact gggcggagtg ggaaggccat
     1261 cccgggttca tcgaggacat cctgcgcttc ctggacaacg tgctgcagga cttcatcgat
     1321 cgggcgccgc ctggaatgga aaaggcgaaa tacgcggcga tgcgcgagcg ttcggttggc
     1381 ctcggggtaa tggggttcca ttcgttcctg caggcgctga acgtgccgtg ggaaagcgtc
     1441 gtcgccaagg tctggaacaa gaaaattttc cgccacatcc gcgcacaggc ggacgcggcg
     1501 tcggtaaaac tggcggagga gcggggcgct tgtccggacg ctctggaata tggggtgaag
     1561 gagcgtttct cgcacaagat gtcgatcgcg ccgacggcat cgatttccat catcgcaggc
     1621 aactcatccc ccgggatcga gccgatcgcc gcgaacgttt tcctgcaaaa gacgctgtcg
     1681 ggaagtttct cggtccgtaa ccggcatttg cagaagctgc tggcggaaaa gggctacgac
     1741 aacgacgcgg tctggtcgtc catcaccacg acgaaaggca gcgtgcagca tttggatttc
     1801 ctgagcgaac aggagaaggc ggtttacaag accgctttcg agttggacca gcgttggatc
     1861 atcgagcatg cggccgatcg tgctccgtcg gtttgtcaga gccagtcggt gaacgtgttc
     1921 ctgccggcga acgtgcacaa gcgggatctg catcagatcc atttcatggc ctggaagaaa
     1981 ggcgtcaaat cgctgtacta ctgccggagt ttgtcgattg cccgggcgga tacggcgagc
     2041 gatcgaggcg tggcgccggc ggcgttcacc ggcgagatga tcggggcgac gctgccggcg
     2101 agtgataagc agggacagtt gccgcttgtg gcaatggggt ccgggaacgc ggcggattat
     2161 gaggagtgtt tggcctgtca atag
//
LOCUS       DASWQN010000169         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2525570, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000169 DASWQN010000000
VERSION     DASWQN010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2525570"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VGC82_02785"
     CDS             complement(<1..1160)
                     /locus_tag="VGC82_02785"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEY0422227.1"
                     /translation="MLTKSPSPEAAVEALIVLHDQAATGLKACLERFFETREPPTAAE
                     RQGFRYPELMVHYEAQGLQPSIARAYAKFQGPGTYVTTVARPAHFRRYLIEQLTHLVR
                     DYGANLEVGISEQEIPYPYVLENGDDLAGDGTTAAELALHFPVPKLALVGDEIADGQW
                     DHTPGHPFPLALFDAVRIDYSLKRLQHYTGTHWSNIQPWILLTNYHRYVGQFIMRGIE
                     QIGSVGPYNALHLPGGGTLKRGMTAEDMNQIVEASLWHRFQMPAYHLSRNDGHGGVTL
                     VNIGVGPSNAKNITDHLAVLRPHCWLMIGHCGGLRQSQRIGDYVLAHAYLRQDRILDS
                     LVPPDIPIPALAEVQVALQDAAASVTGESGEALKQRLRTGTVVTNDDRNWELR"
     gene            1212..1287
                     /locus_tag="VGC82_02790"
     tRNA            1212..1287
                     /locus_tag="VGC82_02790"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1245..1247,aa:Ile,seq:cat)
     gene            1397..>1665
                     /locus_tag="VGC82_02795"
     CDS             1397..>1665
                     /locus_tag="VGC82_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HEY0422228.1"
                     /translation="MPDTTHEQGHIHGADRWKHNGIRVIKGDRLDDNTPQTPGMYRQA
                     AINHARVGAQKIWAGTVSIQPDAKTGVHHHGELESVIYVVRGRARL"
BASE COUNT          348 a          476 c          514 g          327 t
ORIGIN      
        1 cggagctccc agttccggtc gtcgttggtc actacggtgc cggtgcgaag ccgctgtttc
       61 aaggcttcac cgctctcgcc ggtgaccgag gctgcagcgt cctgcagcgc cacctgcact
      121 tcagccagcg ccggaatcgg aatatcgggc ggcactaggc tatcgagaat acggtcttgc
      181 cgcagatagg cgtgggccaa tacataatca ccaatccgct gggattggcg gagcccgccg
      241 caatggccaa tcatcagcca gcaatggggc ctgagcactg ccagatggtc ggtgatgttc
      301 ttggcgttcg atggtcccac accgatgttg acgagagtga cgccgccatg tccatcgttc
      361 cgcgatagat gataggccgg catctggaaa cggtgccaga gagaggcttc gacaatctgg
      421 ttcatatctt cagcggtcat gccgcgcttc agcgttccgc cgcctggcag atggagcgca
      481 ttatacggtc ccacgctgcc gatctgttcg ataccccgca taatgaactg gccaacgtaa
      541 cgatggtaat tggtgagcag gatccatggc tggatgttgc tccagtgggt gccggtgtag
      601 tgttgcaaac gcttcaacga atagtcgatc cgcaccgcat cgaacagcgc caagggaaag
      661 ggatgtcccg gcgtgtggtc ccattggcca tcggcgatct catcgccgac cagggcaagc
      721 ttcggcacgg gaaaatgcag cgccagctcg gctgcggtcg ttccatcgcc cgcaaggtcg
      781 tcaccgttct ccagaacgta gggataggga atttcctgtt cggaaatccc gacttcaagg
      841 ttggcgccat aatcgcgaac gagatgagta agctgctcga tcaaatagcg ccggaaatgc
      901 gccgggcgcg ccactgttgt cacatatgtg cctgggcctt gaaacttggc ataggcgcgg
      961 gcgatcgagg gctgaaggcc ctgcgcctca taatgcacca tgagttcggg ataccgaaac
     1021 ccttgacgtt cggctgcggt tggaggttcg cgggtctcaa aaaaccgctc gagacacgcc
     1081 ttgagaccgg ttgcagcttg gtcgtgaagc acgatcagcg cctcgacggc ggcttccggg
     1141 gaaggggatt tggtgagcac cgaagcgcta tagcatgaca ggaaaaattg ctgtagccac
     1201 tgcctccgcg tgggcccgta gctcaatggt tagagctgac ggctcataac cgtccggttc
     1261 taggttcaag tcctagcggg cccaccacgc agtctggtct ccagtgcaga tttaatttca
     1321 ttttgagaaa ccccattttt cgcgcggcaa ttggcgctat ttgccgtcac cttctgcagc
     1381 cggctggagg ggctgcatgc ctgatacgac gcacgagcaa ggacacattc acggcgccga
     1441 ccgctggaag cataacggca tccgggtgat caagggcgac cggctggacg acaatacgcc
     1501 ccagacgcct ggcatgtacc gtcaggcggc gatcaaccat gcgcgcgtgg gcgcccaaaa
     1561 aatatgggct gggaccgtgt cgatccagcc cgatgcgaag acgggggtgc atcatcacgg
     1621 cgaactcgaa agcgtgatct acgtggtgcg aggcagagcg cggct
//
LOCUS       DASWQN010000170         2957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_546337, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000170 DASWQN010000000
VERSION     DASWQN010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546337"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1653
                     /locus_tag="VGC82_02800"
     CDS             <1..1653
                     /locus_tag="VGC82_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HEY0422229.1"
                     /translation="LLTLPPYGFYWFLLSEVDDWPSLHTPAPEPMPEYQTIVMRQALP
                     DALIAARSVIEREVLPPYLAKRRWFAMKDQVLRSARIALMTRVPAEDAVLLEIETESA
                     SGTARWLLPLGIVWDDTTAAPLPAQLALARVRRGPKVGLLTDAFALPGFPLAVLAGLA
                     QKDIIAIEEGMIKFEPTSRMAAISVPEGVEMIWISAEQSNSSVTVGDIAIIKMFRRIT
                     DGPHPEAEMGRYLTENGFANTPALLGEVVRVDRAGISHALAIAQAFVRNQGDCWTWTL
                     DLLMRGLSDISAGDEATVADAASHADYVRFARLLGQRLGEMHLVLARPTAEPAFAPMQ
                     GDAAAAAALARRVEEQLEAAYRAFEASEEDDRTTMQSARKALFDRLPALAATVKGATL
                     TRIHGDLHLGQVLVCNGDVAIIDFEGEPAKPVDIRRSKDHPLRDVAGMIRSFDYAAAV
                     VKRRSQSSHAHLPDEHVEAFLDSFVQRATESFQAGYREAVSSGDERPPMEAEAELLDL
                     FLIEKAAYEVVYEAANRPTWIDVPMHGLARYTRHLLGVEAVA"
     gene            1720..>2957
                     /locus_tag="VGC82_02805"
     CDS             1720..>2957
                     /locus_tag="VGC82_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0422230.1"
                     /translation="MNDAVVIPPVSPEQAWQLASGSLRDPFAVLGPFETGIGRIVRTF
                     QPGASGVEVLARSNGRRLGVLSSTQPDGLFTGRVDSNEPYFFRIQWSGAFQETEDSYS
                     YDLLLSETDLHLFNEGRLFEIAFTLGANPVVIGGVHGTRFAVWAPNARAVSVIGDFNT
                     WDNRRHLMRLRYPSGIWELFVPRVGPGARYKFAIVAADGTRLADKADPLAKATEAPPA
                     TASIVADPVPYVWHDQTWMQTRAKRHSADAPISVYEVHAASWFRPGPGQVASWDALAE
                     RLVPYVAEMGFSHLELMPVAEHPFGGSWGYQPLGLFAPSGRFGPSEGFCRFVDACHRA
                     GIGVIIDWVPAHFPTDAHGLARFDGTPLFEHADPKEGFHQDWNTYIYNFGRREVQGFL
                     IASGVHWLEHFHVDGLRVDAV"
BASE COUNT          511 a          904 c          962 g          580 t
ORIGIN      
        1 ctgctgacgt tgcccccgta cggcttctat tggtttttgc tttctgaagt tgacgactgg
       61 ccatccctcc acacaccggc accggaaccc atgccagaat atcagaccat tgtcatgcga
      121 caggccttac ccgatgccct catcgcggcg cgttcggtga tcgagagaga ggtcttgccg
      181 ccgtatctcg cgaaacggcg ctggttcgcg atgaaggatc aggtcctgcg ttcagcccgc
      241 atcgcgctga tgacccgggt ccccgccgaa gacgcggtct tgctggaaat cgagaccgag
      301 agcgcatccg gcaccgcgcg gtggctactg ccgcttggca tcgtctggga cgataccacc
      361 gcggcaccct tgccggccca actcgcgctg gcgcgcgtgc ggcgtggacc aaaggtcggg
      421 ctgctgacgg acgcctttgc tttgccgggc ttcccactcg cggttcttgc cggactggcg
      481 cagaaggata tcattgctat cgaagaagga atgatcaagt tcgagccgac gtcgcgcatg
      541 gctgcgatca gcgtacctga gggcgtcgag atgatctgga tctccgccga acagtccaat
      601 tcctcggtga cggtgggcga catcgcgatc atcaagatgt tccgccggat taccgatggg
      661 ccgcatccgg aggccgagat gggccgctac ctgaccgaga acgggttcgc caatacgcct
      721 gcccttctgg gtgaggtggt gcgggtcgat cgggctggaa tttcacatgc cctggcgatc
      781 gcccaggcat tcgtgcgcaa tcaaggcgac tgctggacct ggacgctgga cctgctgatg
      841 cgcgggctat ccgatatcag cgccggggat gaagccacgg tcgccgacgc cgcaagccat
      901 gccgattacg tccggttcgc aaggctgctt ggccagcggc tgggggagat gcaccttgtt
      961 ctggcgcgtc cgactgccga accggcgttc gcacccatgc aaggggatgc cgccgccgcg
     1021 gcggctttag cacgccgggt cgaggaacaa ctcgaggccg cctatcgagc gttcgaggcg
     1081 tccgaggaag atgatcgcac gacgatgcag tcggcgcgaa aggcactgtt tgacaggctc
     1141 ccggcgctgg ctgccaccgt caaaggtgcg accctgacac gcatccatgg cgacctgcat
     1201 ctgggccagg tgctggtctg caatggcgac gtcgccatta tagattttga aggcgagcca
     1261 gctaagcccg tcgatatccg tcgctccaaa gatcatccgt tacgcgatgt cgccgggatg
     1321 attcgttcgt tcgactacgc cgcggccgtc gtcaaacgac gcagccagtc cagtcacgcg
     1381 catctgccgg acgaacatgt cgaggcattc ctggacagtt tcgtgcaacg ggcaaccgag
     1441 tcgttccagg ccggttaccg cgaagcggtt tcgtccggtg acgaacggcc gcccatggag
     1501 gctgaagcag agttgctcga tttgttcctg atcgaaaagg cagcgtatga agtggtctat
     1561 gaggcggcaa accggccgac ctggatcgac gtgccgatgc acggactagc gcgctacacg
     1621 cggcatttgc tgggcgtcga ggctgtagcg tgacccggag ccgcggcgtg acccggagcc
     1681 gcagcgtgac tggggccgta gcgtgatcgg ggccggggca tgaatgacgc cgtggttatt
     1741 ccgccggtct cgcccgagca ggcttggcaa ctggccagtg gcagcctgcg ggacccattc
     1801 gcggtcctgg gccccttcga aaccggaatt ggccgaatcg tgcggacgtt tcagcccggc
     1861 gcatcgggcg tggaggtgct cgcccggtcg aacggcaggc gcctgggggt attgtcatcc
     1921 acccaaccgg acggattgtt caccgggcgg gtggacagca acgaaccata tttcttccgt
     1981 atccagtggt ccggtgcgtt ccaggagacc gaggactcgt atagctacga cttgttgctc
     2041 agcgagaccg acctgcacct cttcaatgaa gggcgtcttt ttgagatagc ttttaccctg
     2101 ggcgccaatc cggttgtgat cggaggggtc cacggcaccc gttttgccgt ttgggcaccc
     2161 aacgcgcgcg ccgtttcggt gatcggggac ttcaacacct gggacaaccg gcgccacctg
     2221 atgcggctgc gttatccgtc cggcatttgg gaattgtttg ttccgcgcgt cgggccgggt
     2281 gctcgatata aatttgccat tgtagcggcg gatggcaccc ggctggccga caaggcggat
     2341 ccgctggcga aggccacgga ggcgccgcct gccaccgcat cgatcgtcgc cgacccggta
     2401 ccctatgtct ggcatgatca aacctggatg caaacccgag ccaaacggca cagcgcggac
     2461 gcgccgatct cggtttacga ggtccacgcc gcttcctggt tccggccggg cccgggacag
     2521 gtcgcaagct gggacgcgtt ggctgagcgg ttggtcccat acgttgccga gatgggtttt
     2581 tcccacctcg agctgatgcc ggtggcggag catccgttcg gcggatcatg gggttaccag
     2641 ccgttgggtc tgttcgcgcc gtccggccgc ttcggaccat cggaaggttt ctgccggttt
     2701 gtggatgcct gccatcgcgc tggcatcggg gtgatcatcg actgggtccc ggcgcatttc
     2761 ccaaccgatg ctcacggact cgcccgcttc gacggcacgc cgctgttcga acatgccgat
     2821 cccaaggagg gatttcacca ggactggaac acctacattt acaatttcgg gcggcgggaa
     2881 gtgcagggat tcctgatcgc cagcggtgtc cattggttgg agcatttcca cgtcgatggc
     2941 ctgcgcgtcg atgcggt
//
LOCUS       DASWQN010000171         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1733886, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000171 DASWQN010000000
VERSION     DASWQN010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733886"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..151
                     /locus_tag="VGC82_02810"
     CDS             17..151
                     /locus_tag="VGC82_02810"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422231.1"
                     /translation="MDVVAEGTSLRLTQRQDYASYLTHNNNNIDALIAAMKSQVSQGS
                     "
     gene            complement(220..705)
                     /locus_tag="VGC82_02815"
     CDS             complement(220..705)
                     /locus_tag="VGC82_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0422232.1"
                     /translation="MLRTGRLVLTPVGGADLGDLRAIKSDPLVYAVMLGGVRDHTQTA
                     EDLARDVAAWGRFGFGMWAVREAGRFVGVVGLQHRADGRGIALRFALWPEAQGRGLAR
                     EAAYATLRFGHDRAGLPRIVAVARESNFGSRIVLGGIGMKETSTFGQFGYTMILYESL
                     S"
     gene            complement(745..1488)
                     /locus_tag="VGC82_02820"
     CDS             complement(745..1488)
                     /locus_tag="VGC82_02820"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0422233.1"
                     /translation="MDAETGIQSDIDYQARLDLAAVYRLLALHGWGDVIFNHAAMRSP
                     SNPRHFLIKRHELLYTEVTASNLVEVSMDDDLDERAGVNRPGFTLHSGVLRGRPDVQC
                     SVHVHPEEGIALSGMKQGLRPLSQNAMRFYNRVGYHDYEGLTDSFDERERIVAALGKN
                     RALVMRHHGITTVGETAREAFSLMKEFVRAARIQLQMQATGAALLEIPPEVCERVAAQ
                     YEKHDRGRASAEWPAYLRQLDTIDPGYRA"
     gene            1706..2107
                     /locus_tag="VGC82_02825"
     CDS             1706..2107
                     /locus_tag="VGC82_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0422234.1"
                     /translation="MPIKKGYKHLLAEANAEIQTLPAGQAAELLGKPDVVFVDLRDPR
                     EIEREGKIPGAIACTRGMLEFWIDPESPYAKPVFSEPKRFIFFCAGGWRSALAAKTAQ
                     DMGLENVAHVDGGFVAWKQTGAPVEAPKPKT"
BASE COUNT          456 a          747 c          676 g          411 t
ORIGIN      
        1 gacacagccc aggatcatcg acgtcgtggc cgagggaacc tcgcttcggc tgacccaacg
       61 ccaggactat gcgtcctacc tgacacacaa caacaacaat atcgacgcgc tgatcgcggc
      121 gatgaaaagc caggtttcgc aaggcagtta gatcattccc aggcggaccg ctgggcgcgg
      181 gatcagcgac ggctgagcgc gatgatcggg ccatcctgct caggacaggc tctcataaag
      241 gatcatcgtg tagccgaact gcccgaaggt gctggtctcc ttcatgccga ttccgcccag
      301 cactatccgc gacccgaagt tgctctcacg ggcgacggcg acaattcgcg gcaaccccgc
      361 gcgatcgtgg ccgaagcgca gcgtggcgta cgccgcttcc cgagccaggc cccgcccttg
      421 cgcttctggc cagagtgcaa aacgcaaggc gatccctcgc ccatccgctc gatgctgcaa
      481 tccgacgacc ccgacaaatc ggcccgcttc ccggaccgcc cacatcccga atccgaagcg
      541 cccccaggcg gcaacgtcac gggccaggtc ctcggcggtc tgagtgtgat cgcgaacgcc
      601 gccgagcatt accgcataga ccagtgggtc ggattttatc gctcgaaggt ctccgaggtc
      661 ggcgccgccg accggagtga gaaccagccg acccgtgcgt aacacccaag ccgggttcat
      721 gacggaccca gctggttgat gcgatcaggc gcggtaaccg gggtcgatgg tgtcgagttg
      781 tcgaagatag gccggccact cggccgaggc ccgcccccga tcatgcttct cgtactgcgc
      841 ggcaacccgt tcacatacct ccggagggat ctccagcagt gcggccccgg tggcttgcat
      901 ctggagctgg attcgcgcgg cgcgaacgaa ctccttcatc aaggagaacg cctcgcgtgc
      961 ggtctccccg accgtggtga tcccgtgatg ccgcatgacc agcgcccggt tcttgcccaa
     1021 cgcggcgacg atgcgctcgc gttcgtcaaa gctgtcggtc aggccttcat agtcgtgata
     1081 gccgactcgg ttgtagaacc gcatggcgtt ctgcgacagc ggtcgcaagc cttgtttcat
     1141 gcccgagagc gcgatgcctt cctctggatg gacgtggacg gaacactgca catccggccg
     1201 tccgcgcagg actccgctgt gcagggtgaa gccaggacgg ttgactcccg cccgctcgtc
     1261 caggtcgtca tccatgctga cctcgaccag gttggaagcg gtgacctcgg tgtacagcag
     1321 ttcgtgccgc ttgatcagga aatggcgcgg attggatggc gaacgcatgg cggcatggtt
     1381 gaagatcaca tcgccccacc cgtgcaaagc caacagccgg taaacggcgg caagatccag
     1441 cctggcctga taatcgatgt cggattggat tccggtttcg gcgtccatcg ctgtttctcc
     1501 cgttcaggct gtttctcccg ttcacggaac tagatactca accgtgggca taggggcaaa
     1561 ttccagggta ttcgccgccc atcggcgtcg ataagaatca gggacgccac ctggcaccgt
     1621 ggccaaagca tccgacaacg atctatattc gtcgcggaac gctgcttaat tgggttcgca
     1681 gcatccaggc caaggaagca acactatgcc gatcaaaaaa ggctacaagc acctgcttgc
     1741 ggaagccaat gctgaaattc agacacttcc cgccggacag gccgccgagc ttctgggcaa
     1801 acccgatgtg gttttcgttg atctccgcga cccccgggag atcgagcgcg aaggcaagat
     1861 accaggtgcc attgcctgca cccgaggaat gctcgaattc tggatcgacc cggaaagccc
     1921 ctacgccaag ccggtgtttt ccgaaccgaa gcgattcatc ttcttctgcg ctggcggctg
     1981 gcgttcggcg ctcgccgcga agaccgcgca ggacatgggt ctggaaaatg tcgcccatgt
     2041 cgatggtggc ttcgtcgcct ggaagcaaac cggcgccccg gtcgaggcgc caaaacccaa
     2101 aacctgacgt ctgatcaggt tcgcgatccc ggcgacagca attccgctgg caaccacacc
     2161 ctctgcacgc gccacgcgcc acgccgcagg tccatctcca gcttcactgg ctccgtttgc
     2221 ccgggcgctt gcaggctgac catgaacgtc gttgggctgt cgaagaaggc ccaattgaca
     2281 tgcacgtcgg
//
LOCUS       DASWQN010000172         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1602001, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000172 DASWQN010000000
VERSION     DASWQN010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602001"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1398)
                     /locus_tag="VGC82_02830"
     CDS             complement(232..1398)
                     /locus_tag="VGC82_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0422235.1"
                     /translation="MNTMDRVVTLRGCRAAGAIAVLAALAACGTVPPHQDAVRESARY
                     AAHAKHNYVPPGPSEDPWGPYIREASGRFDIPESWVRSLMRVESGGREYVDGQLITSG
                     AGAMGLMQVMPVTYDELRARYNLGDDPFDPHDNIMAGVAYMREMYELYGSPAFLAAYN
                     AGPSRLDDYLSNVRPLPDETRRYVAMIGPNLRGVFPNNRSAGEELAMNALPINIPPGR
                     RYARPVMLAGAKTGGGGRVPQRAPVEVAQLAEPQHRGGNRSGQQVVLVLPPVAPPAGP
                     ASGSRGFRLIASANAADAAPAHHGPAAPGQWAIQVGAFGNQGQANSALLMAHSHAQVE
                     LAVAHPFVASVHQGRTILWRARMTGMSRETAVQACEKITRGRSSCIVLSPDTQS"
     gene            1697..>2392
                     /locus_tag="VGC82_02835"
     CDS             1697..>2392
                     /locus_tag="VGC82_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0422236.1"
                     /translation="MSSAVTTIAPATELNLRAEMTFAARNRWAMADVASGIRLWRLAW
                     ALGWLDIRLRYRGSMLGPFWLTISTGVMVTALGLLYSTLFNINVRDYLPFLALSQVLW
                     GFLAAIVSEGCSIFTDAEGVIRSVRMPFFVFSMRALIRNSIALGHNILVIVVVFAIFS
                     MWPGMDALMAIPGLMIWVVDALALTLMLGAFCARFRDIQPIVNSVMQIAFFMTPVIWK
                     PDQLGPDSASKLAF"
BASE COUNT          414 a          743 c          767 g          468 t
ORIGIN      
        1 gccaggatgc gaactgccgc ctggaggcag aaacggcggg cggcatcgcc acgaacgcgc
       61 cttcggaagt tctgagcctc aagggaagca gcaaggcgct ggccggcaag cttggggtca
      121 gagggcagaa tacgacattt agcctcaaga ccggagaggc ggacgactag cgcggctgtc
      181 tgaacgcgcc cttccacgtg ccatggacgt tccgcgcacc gcaacctcgc cttatgactg
      241 agtatccggc gacagcacga tgcagctcga cctgccccgg gtaatcttct cgcacgcctg
      301 tactgcggtc tcacgcgaca tcccggtcat ccgagcacgc cacaggatag tgcggccttg
      361 atggacgctg gcgacgaatg ggtgggcaac ggcgagttcg acctgcgcat ggctgtgcgc
      421 cattagcagc gcgctgttgg cctggccttg attgccgaag gcgccgacct ggatcgccca
      481 ctggccgggc gcggcgggac cgtggtgggc aggtgcggcg tcggctgcgt tggccgaagc
      541 aatcagacgg aagccgcgcg atccgcttgc gggcccagcg ggaggagcca ctggaggcag
      601 caccaggacc acctgctgcc cggatctgtt tccgccgcga tgctgcggtt cagccagttg
      661 cgcgacctcc accggtgccc gctgcggcac gcgtccgccg ccgccggtct tcgcacccgc
      721 caacatgacc gggcgggcat agcgtctgcc gggcggtatg ttgatcggca gcgcgttcat
      781 cgccaactcc tccccggcgg agcggttgtt cgggaaaacg ccccgcaggt tggggccgat
      841 catcgcgacg tagcggcggg tttcgtcagg cagcggccgg acgttggaca ggtaatcgtc
      901 caagcgcgat gggccggcat tgtacgccgc cagaaatgcg ggcgagccgt agagttcgta
      961 catctcccgc atataggcga cgccggccat gatgttgtcg tgcggatcga acggatcgtc
     1021 tccgaggtta tagcgggccc ggagctcgtc atacgtgacc ggcatcacct gcatcaggcc
     1081 catcgcgccg gcgccggacg taatgagctg gccgtccacg tattccctgc cgcctgactc
     1141 cacccgcatc agcgagcgaa cccagctctc cggtatatcg aatcttccgg atgcctcgcg
     1201 gatataggga ccccaaggat cttccgacgg tccaggtggg acgtagttat gcttggcatg
     1261 ggccgcgtag cgggcggact cgcgcaccgc gtcctggtgc gggggaacgg tgccgcacgc
     1321 ggcgagggcg gcgagcacgg caatcgcgcc ggcggcacgg cacccgcgaa gcgtgacaac
     1381 tcgatccata gtattcataa cgatggcgtc tccgtggcag cgcgacagtt tcatatgcgg
     1441 ctgcctagcg gtcgccgatt atctaatgac taaagaagat actctaagcc attaacccac
     1501 ttccatcttt cctgcaagta atctcgccct ggtggcgtaa accgctttcg ctgggaattc
     1561 acgcactacg ggcatcaggc agccttcaga ttcaagaagt ctgtcctccg caacatcttt
     1621 cacgattgcg gcccggatgc ggcggcagtt acggtctgtt gtgccacatc cctgaatggg
     1681 gtaagcgccc cgcgacatga gcagcgccgt aacaacgatt gcccccgcca ccgaattgaa
     1741 cctgcgagcg gagatgacct tcgccgcgcg caaccgctgg gcgatggcgg atgtcgcgag
     1801 tgggatccgc ctttggcggc tggcttgggc attaggatgg ctggacatcc gcctgcgcta
     1861 tcgcgggtcg atgctggggc cattctggct gaccatatcc accggcgtaa tggtgacggc
     1921 gctgggtctg ctgtactcga cgttgttcaa tatcaatgtt cgggattacc tgccgttcct
     1981 ggccctttct caggtacttt ggggattcct ggctgccatc gtttcagaag gctgctcgat
     2041 ttttaccgat gccgaaggcg tgatccgatc ggttcgcatg ccgttcttcg tcttctccat
     2101 gcgcgcgctg atccgcaaca gcatcgccct gggccacaac atccttgtga tcgtcgtggt
     2161 gttcgcgatc ttctccatgt ggccgggcat ggatgcgttg atggcgattc ccggcctgat
     2221 gatctgggtg gtcgacgcgc tggcgctgac cctgatgctg ggggcgttct gcgcccgctt
     2281 ccgggacatc cagccgatcg tgaacagcgt gatgcagatt gcctttttca tgactccagt
     2341 gatctggaag ccggatcagc ttggcccgga cagtgccagt aaattggcgt tc
//
LOCUS       DASWQN010000173         4291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_546545, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000173 DASWQN010000000
VERSION     DASWQN010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546545"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VGC82_02840"
     CDS             complement(<1..500)
                     /locus_tag="VGC82_02840"
                     /inference="COORDINATES: protein motif:HMM:NF021141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HEY0422237.1"
                     /translation="MPASEPSANEAVVYVIGRGWHTDIGLPVEEIAGPLAALKADFPG
                     VRYLTFGFGERQFLVNRSTDFGAMLRALLPSQSALLVTALGATPEAAFGRANVVVLGV
                     SRAGLERIEDGIWRQLVSPPGDDPVRLAEGPYDGSVFYASQGSYNAFFTCNTWTARTL
                     RAGGLA"
     gene            805..2025
                     /locus_tag="VGC82_02845"
     CDS             805..2025
                     /locus_tag="VGC82_02845"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0422238.1"
                     /translation="MIPRIELDPTAPAPLDGIRVVDLSRLVAGNMVSLQLADQGAEVI
                     KIEDPKVGDPLRAWRVKGLSLHWKVYARNKKSLAINLRPQAGRDALLDLLASSQVLIE
                     NYRPGTLEAMGLGPETLHARNPSLIIVRITGFGQDGPYRDRPGFGTLVEAMSGFAAKN
                     GFGDRPPVLPPLALADMVSGLYGAYAVMVALRVVEHGGRGQTIDLPLLEPMISVLGPD
                     AASYRVSGEKPQRTGSRSLTTSPRNVYGTSDGRFVAISASIQAMAERLFRAIGRADVI
                     DDPRFRTNTDRVRNIDACDQIVADWIGERTLAENMAVFEAAEVTATPIFEIDQLLDDA
                     HVQARGVFVEVPDEEAGSVLMHNVIPRLSETPGRLRSPAPRLGQHTRSVLESIGYDAA
                     RLATLAAEGVIKEG"
     gene            2028..2918
                     /locus_tag="VGC82_02850"
     CDS             2028..2918
                     /locus_tag="VGC82_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY0422239.1"
                     /translation="MRDDLPVWRSLMFVPVTAEKFVRTGADRGADAIILDLEDAVAPS
                     QKAHARTLIEGAIPQVSRNGADVVVRVNRPWRLLVRDLEAAVIPGVTALMLTKVDSPE
                     HVLACAEIVAELEAERGLPLGGTKFIALVENANGFFRIEQIAKSHPRVVGLSLGSEDF
                     AADVGMQSEPEGLFYPKQHTIFAARAAGIMPMGFIGSIADFRDQEAFRAIVRRSRRLG
                     FMCASAVHPLQVTVLNEEFSPDPAEVQRAEKMIAAYDDAYAKGLGAVQYEGAMIDVPV
                     VERARSVVKRAIALRDRLRA"
     gene            complement(2959..3879)
                     /locus_tag="VGC82_02855"
     CDS             complement(2959..3879)
                     /locus_tag="VGC82_02855"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0422240.1"
                     /translation="MHDTAYEHGRLFFELYASSACRTVVELGSQDVNGSLRDHCWPGV
                     HYIGLDMVAAKGVDLVVDPGEPLPLRSETADAVVTSSAFEHDVCFWDTFLDLIRILRP
                     GGLLYVNVPSNGGFHRHPLDCWRFYPDAGAALVQWAARRGFQVELVESFIGRPETERW
                     ADFVAVFRRAGGPPLIRKGRIADRVACINIHDLGMAADAPLQAENADMPDMQLQTDLR
                     RELAELRAATVEDLAAAAHRGATLSEELAASRTEADGLRQKLSKMERRTAELEAECAD
                     TGEELSAMQNSVSWRLTAGPRRLMQALRGR"
BASE COUNT          812 a         1304 c         1415 g          760 t
ORIGIN      
        1 atcgccagcc cgcccgcccg caaagtccgc gcagtccagg tgttgcaagt gaaaaacgcg
       61 ttgtagctgc cctgcgaggc atagaagaca ctgccgtcgt acggaccctc ggccagccgc
      121 actggatcgt cgcctggtgg agagacaagt tgccgccaga tgccgtcctc gatgcgttcg
      181 aggccggcgc gggatacgcc cagcaccacg acgttcgccc gaccaaaagc cgcttccggc
      241 gtggcaccca atgcagtcac cagtaacgca ctctggctcg gcaacaaggc gcgcagcatt
      301 gcgccgaagt cggtgctccg gttcaccagg aattgccgtt cgccgaagcc gaacgtcagg
      361 taccgcacgc cggggaaatc agccttcagt gccgccagcg ggccggcgat ttcctcgact
      421 ggcaagccga tgtcggtatg ccagccgcgc ccaatcacat agacgactgc ctcgttcgcc
      481 gacggttcgg atgccggcat tgaacacgat ccggccaaaa cgccgagcgc gaggcaggca
      541 aaggcagccc ggcggtatga catgtggccg atcgcctctg cttggacgcc tggagcaaga
      601 atgcgtggag cctccgccgc ccggtgaagc atgaacaccg gcagacatcc agatcattct
      661 caacccggac gcacgggctc aacccggacg cgcggacgat atacccattg cggcgttcga
      721 tctggggcaa ggagcccgcc gccatgcagc ccttgcgcac cggccgcgtc tgacgggaca
      781 ataccgcgtc caagaaggcc gccaatgata ccacgcatcg aactcgaccc gactgcacct
      841 gccccgctgg atggcatccg cgtcgtggac ctgtcccgcc tcgtggccgg caacatggtc
      901 agcctgcagt tggccgatca aggtgccgaa gtcatcaaga tcgaggaccc gaaggtcggc
      961 gacccgctgc gtgcctggcg cgtcaagggg ctctcgctgc attggaaagt ctatgcgcgg
     1021 aacaagaaaa gcctggcgat caacctgcgt ccacaggccg gccgcgatgc ccttctcgat
     1081 ctgctggcca gcagccaggt gctgatcgag aactatcggc ccggcacgct ggaggcgatg
     1141 ggactgggtc cggaaacgct gcacgcgcgt aatccgtccc tgatcatcgt gcgcatcacc
     1201 ggatttgggc aagacgggcc gtaccgtgat cggcccgggt ttggtaccct ggtcgaggcg
     1261 atgtcggggt tcgcggcaaa gaacggattc ggcgaccggc ctccggtgct gccgccattg
     1321 gcgctggcgg atatggtttc gggattgtac ggcgcctatg cggtgatggt ggcgttgcgc
     1381 gtggtggaac atggcgggcg aggccagacg atcgatcttc cgctgctcga accgatgatt
     1441 tccgtgctgg gtccggatgc ggcaagctat cgcgtttccg gcgaaaaacc gcagcggact
     1501 ggcagccggt cgctgacgac atcgccacga aacgtgtacg gcacgagcga cgggcggttc
     1561 gttgccatct ctgcctctat ccaggccatg gcggagcggc tgttccgagc gattgggcgg
     1621 gcggacgtga tcgacgaccc gcggttccgc accaacaccg accgggtgcg gaatatcgat
     1681 gcctgtgacc agatcgtcgc ggactggatc ggggagcgca cgctggcgga aaacatggcg
     1741 gtgttcgaag cggccgaggt gacggcgacg ccaatctttg aaatcgacca gttgctcgac
     1801 gatgcgcatg tgcaggcgcg cggcgtgttc gtggaagtgc ccgacgagga ggccggatcg
     1861 gtgttgatgc acaatgtcat cccgcgactg tcggaaacgc cgggacgatt gcgaagcccc
     1921 gctccccggc tgggacagca cacgcgctcg gtcctggaat cgatcggtta cgatgcggct
     1981 cggctggcca ccctggcggc cgagggggtg atcaaggagg gctgacaatg cgtgacgatc
     2041 tgccggtatg gcggtccctg atgttcgtac cggtgaccgc ggagaaattc gtccgcaccg
     2101 gcgccgatcg cggcgcggac gcgatcatcc tggatctgga ggatgcggtt gctccttcgc
     2161 agaaggcgca cgcacggacg ctgatcgagg gcgccatccc ccaggtgtcg cgcaacggcg
     2221 ccgacgtggt ggtgcgggtg aaccggccgt ggcggctgtt ggtgcgcgac ctggaggcag
     2281 cggttatccc cggtgtgacg gcgctgatgc tgaccaaggt ggatagcccg gaacatgtgc
     2341 tggcgtgcgc cgaaatcgtt gccgagctgg aagcggaacg cgggctgccc ttgggtggca
     2401 cgaagttcat cgcgctggtg gagaatgcga atggcttctt ccgcatcgag cagatcgcga
     2461 aatctcatcc acgcgtggtg gggttgagtc tgggttccga ggattttgcc gccgatgttg
     2521 gcatgcagtc cgagccggag gggctgttct atcccaagca gcatacgatt ttcgccgctc
     2581 gggcggccgg gatcatgccg atggggttca ttggatcgat cgccgacttc cgggatcagg
     2641 aagcctttcg agcgatcgta cggcgatccc ggcggctggg atttatgtgc gccagcgcgg
     2701 tacatccgct gcaggtgacc gtactgaacg aggaattttc tccggacccg gccgaagtgc
     2761 agcgggcgga gaagatgatc gcggcctatg acgatgccta tgcaaaaggg ttgggcgcgg
     2821 tccagtatga aggcgcgatg atcgacgtac ccgtcgtgga acgggctcgt tcggtggtga
     2881 agcgggccat cgcattgcgg gaccgcctga gggcctgaca gccacgggac ggaaggaggt
     2941 tcgggaacgg ggccggctct aacggcctcg taacgcctgc atcagccggc gcggaccggc
     3001 ggtcaggcgc catgaaacac tgttttgcat ggcactgagt tcctccccgg tgtcggcaca
     3061 ttcggcttcg agttcggccg tccgcctctc cattttactc aatttctggc gcaggccgtc
     3121 agcctcggtc cggcttgccg cgagttcttc ggacagcgtc gcgccgcgat gcgccgcggc
     3181 ggccaaatcc tccactgtcg cggcgcggag ttcggcaagt tccctgcgga ggtcggtctg
     3241 aagttgcata tccggcatat cggcgttctc cgcttgaagc ggcgcgtcgg ctgccatgcc
     3301 taggtcgtgg atgttgatgc aagcgacgcg atcggcgatg cgtcctttac ggatcaacgg
     3361 cggcccgcct gccctgcgga agacggccac aaaatcggcc cagcgctcgg tttccggccg
     3421 tcctatgaaa gattccacca gttccacctg gaaaccccgg cgggcggccc attgcaccag
     3481 ggcggccccg gcgtcgggat agaagcgcca acagtccagc gggtggcgat ggaaaccgcc
     3541 gttgctggga acgttgacat acagcaggcc gccggggcgc agaatgcgaa tcagatccag
     3601 gaacgtgtcc caaaagcaga catcatgctc gaaggccgaa ctggtcacga cggcatcggc
     3661 agtttcagac cggagaggaa gcggctcgcc gggatcgacg acgagatcga ctcctttggc
     3721 cgctaccatg tccaggccga tatagtggac gccaggccag caatgatcgc gcagcgagcc
     3781 gttgacgtcc tgacttccaa gttcgacgac cgtgcggcag gcactcgacg cgtagagttc
     3841 gaaaaatagc cggccatgtt cgtaagctgt gtcgtgcacg ttcgtttcga ccttcggtag
     3901 agttctttaa taacctgcta tcatcaacgg ctacaaacac tgataagcgg tgaacccgac
     3961 atcgcacccg tgatcgtgcg cgaagatcga cggctgcgca cagcgagtgg ctatgcccac
     4021 ctctatgatc gaccagcatg tcctagcggc ataaggtctg gtcgagttgc cactgcaagc
     4081 ttggaatcac gagccttagc cagacgcgat acggatccga aattgaagac gtcagtgcct
     4141 cgaccaattc cctgccgaca tcaaattcaa aatgcattga aacagaagca ggctgcgatt
     4201 accgtgcggc atggtcggcc acccttcaag ccatatcgcc ggttccatgt ggcggctgcg
     4261 acagttgctc ggtggtagtc cagcgtcggg c
//
LOCUS       DASWQN010000174         4264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2130127, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000174 DASWQN010000000
VERSION     DASWQN010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2130127"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..535
                     /locus_tag="VGC82_02860"
     CDS             371..535
                     /locus_tag="VGC82_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEY0422241.1"
                     /translation="MPKRVAANVSLTTELAAFVAAKVASGQYSSASEVVRAALRLLVA
                     SEDLPAKPIE"
     gene            552..3755
                     /locus_tag="VGC82_02865"
     CDS             552..3755
                     /locus_tag="VGC82_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0422242.1"
                     /translation="MPDSSPGAVAAPGNLSDLHRLVADRLGVLPNFFCTAALVPGLID
                     RLWDFAKSSYFDNPLPSLFKERLFVHLSRFCEVRYCIIRHVGFLVGQGNPAGDANCPP
                     QDIEQVVALLRRPVPSEAEFVKALQRLQAAPFRAQEMPEPATQGEADLFDALTLIFLE
                     PRQSALARRAVASAVGEANLEILTALLAFIRTAHYWTETHPDLEYEPDMLGLMSQHAE
                     LAQLLLNQSEAERTWSPMERARMLAALEERDAQQRLLLSLVQAQREADDPTRIMVIAA
                     EAVGRHLGTSVAGFVVPGEGAVTVSASWPAAGSAAIGSLAASGAGVGIVADLQAGRTL
                     RIADTEADPLTAGLGLAEAGLRALVGVPLLRNGRWQGSFVAGHAGVRHWTDAEVALIQ
                     DAGDRTADAAERARAETALRASEEMFRKALEIGTVGIIFFDAAGDITNANDAFLRMSG
                     FSREDQIAGRLRWDAMTPAEWMPASLRAIDELVTLGSTTPYEKEYIRKDGSRFWALFA
                     AKLLGETAGVEFIIDITERKNAEIALQETERRLRTLVEGIPQLVWRAGQAGEWSWVSP
                     QWTAYTGQSTEDSLGDGWLAALHPADRPGALAAWEIAEADGLFEVDYRIRHAKSGSYR
                     WHQTRGIPVHNEAGRIAEWLGTSTDIDDQMSARAVLMRGSVELETRVAERTAELEQAL
                     ATLRDEVRARAQAEEQLRQSEKLKAIGQLTGGIAHDFNNMLQAISSSLSMILTRVQQG
                     RTADVETYVERAQKGAARAAGLTHHLLAFSRQQTLAPKPVSLDRIVRDMEDMIRRTVG
                     PGVQVELQLSDEKWLVMCDPNQMESALLNLCVNARDAMPDGGWLTISTQELNLTEADL
                     SGVEDAPPGPYTVLVVSDTGTGMPPDVVAHIFEPFFTTKPIGQGTGLGLSQIYGFVRQ
                     SGGFVQVETSPGAGTTVRLLMPFHEINPHSDPGVLPALGRTVLLVEDEPDVRELTAEH
                     LRDFGYRVLEADNAAAALRLVQAGTHIDLLLTDFGLPGGMNGRQLAEAVLQRHVGLPV
                     IVITGYAAGEPMRGMEVLRKPFELAALTDLVKARLDP"
     gene            4007..>4264
                     /locus_tag="VGC82_02870"
     CDS             4007..>4264
                     /locus_tag="VGC82_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422243.1"
                     /translation="MSGTADRANVVIQPPLAWALAFVAGLAVDWFYPSPFVPTAIPRA
                     WIGGGVFAVGLALAIWAIVAIRKAGTRVEANKPTTAIVADGP"
BASE COUNT          761 a         1391 c         1403 g          709 t
ORIGIN      
        1 agacgaaccc aaccggaacc gcgctgattc tggaaaacca ggatccgttc ggaatctccg
       61 cttcgagttc atgattaagc agtgtgacct gccgcggtga tcgtgacgac caacctgccg
      121 ttctccgagt ggtcaccctc aatccgcggc tgtgtaagcg gcacctgccc ggctgctgca
      181 cgacgatgcg gcgacgctgg cgttcgttga cggcctcatt ctgtcagacg acgaaaaact
      241 ttagttacgc agaatcacgc aactacccaa agcgccgtcc ccgtagccac gcttttcacg
      301 ttgcagtgtt tgctacccgg atcctgtgtt acagactggc accactattc ctggcctgcg
      361 atagcgaggc atgcccaagc gcgttgcggc gaatgtctcc ctgacaaccg agctggcagc
      421 cttcgttgcc gcgaaggttg cctctggcca gtacagcagt gccagcgagg tggtcagggc
      481 cgctctgcgg cttctggtgg cgtcggagga tctcccggcg aaaccgatcg aatagtctga
      541 tgaggtccac aatgcccgat agcagtcctg gcgcggtcgc tgcgcccggg aacctcagcg
      601 atctgcaccg ccttgtcgcc gaccggctcg gcgtcctgcc gaacttcttc tgcaccgccg
      661 cgctggtgcc cgggttgatc gaccgtctct gggatttcgc caagtcgtca tatttcgaca
      721 acccgctccc gtccctgttc aaggagcggc tgttcgtcca cctgtcccgc ttctgcgagg
      781 ttcgctactg catcatccgc cacgtcggct tcctggtcgg tcagggcaac ccagcaggtg
      841 acgcgaactg tccgccgcag gacatcgagc aggtcgtggc cctgctccgc cgtccggtgc
      901 cgagcgaggc tgagttcgta aaggcgctgc agcggctgca ggccgccccc ttccgcgcgc
      961 aagagatgcc cgaacccgca acccaaggcg aggccgacct gttcgatgcc ctcaccctga
     1021 tctttctgga gccgcgccag tccgcgctgg ctcggcgcgc tgtcgccagc gcagtcggcg
     1081 aggccaatct ggagatcctc accgcgctgc tggccttcat ccgcaccgcg cactactgga
     1141 ccgagacgca cccggatctg gagtacgagc cggacatgct cgggctaatg agccagcacg
     1201 ccgagctggc gcagctgctg ctcaaccaat ccgaagccga gcgtacctgg tctccgatgg
     1261 agcgcgcgcg catgctcgcc gcgctcgagg aacgcgacgc ccagcagcga ctgctgctaa
     1321 gcttggtgca ggcgcagcgc gaggcggacg atccgacccg catcatggtg attgccgcag
     1381 aggcggtcgg acgccatctc ggcacaagcg tggccggctt cgtcgtccct ggggagggcg
     1441 cggtgaccgt ctcggcgtcc tggccggccg cggggtcggc ggccatcggt tccctcgccg
     1501 cctccggtgc gggcgtgggt atcgtggcgg acctgcaggc gggacgcacg ctccgcatcg
     1561 ccgacacaga agctgacccg ctcaccgcag gactggggct cgccgaggcc gggctgcggg
     1621 cactggttgg ggtgccgctg ctgcgtaacg gccggtggca gggcagcttt gtggccggcc
     1681 atgccggcgt ccggcactgg acggacgccg aggtcgcgct gatccaggat gcgggcgacc
     1741 gcaccgcgga tgcggcggag cgcgcccgcg ccgaaacggc gttgcgcgcg agcgaggaga
     1801 tgttccgcaa ggcgctggag atcgggacag tcggcatcat cttcttcgac gctgccggtg
     1861 acatcaccaa cgccaacgac gcgttccttc gcatgagcgg gttcagccgg gaggatcaaa
     1921 tcgccggccg gctgcgttgg gacgcgatga caccggcgga atggatgccg gcatccctcc
     1981 gcgcgatcga cgaactggtg acgctcggca gcacgacgcc ctacgagaag gagtacatcc
     2041 gcaaggacgg ctcccgcttc tgggcattgt tcgccgccaa gctgctgggc gagacggcag
     2101 gcgtggagtt catcatcgac atcaccgagc gcaagaacgc cgaaattgcc ctgcaggaga
     2161 ccgagcgccg gctgcgcacg ctggtggagg gcatcccgca gctggtctgg cgcgccgggc
     2221 aggcgggcga gtggagctgg gtcagccccc aatggacggc ttatacagga cagtccaccg
     2281 aagacagcct gggcgatggc tggctggccg ccctgcaccc agcggaccga ccgggcgcat
     2341 tggccgcatg ggagattgcc gaggcggacg gcctgttcga ggtggactac cgcatccgtc
     2401 acgcgaagtc cggctcctac cgttggcacc agacccgcgg catcccggtg cacaatgagg
     2461 ccggccggat cgccgagtgg ctcggtacct cgaccgacat tgacgatcaa atgtccgccc
     2521 gcgctgtgct catgcgcggc agcgtagagc tggaaacgcg ggtcgcggaa cgcaccgccg
     2581 aactggagca ggcccttgcc acgttgcgcg acgaggtgcg ggcgcgcgcg caggccgagg
     2641 agcagctgcg gcagagcgag aagctgaagg ccattggcca gctcaccggc ggcattgcgc
     2701 atgacttcaa caacatgctg caggccatca gcagcagcct cagcatgatt ctcacccggg
     2761 tccagcaagg ccgaactgcc gacgtcgaaa cctacgtgga acgtgctcag aaaggcgcag
     2821 cgcgggcagc gggcctgacc caccatctgc tggccttttc gcgccagcag acgctggcgc
     2881 ccaagcccgt gagcctggac cgcatcgtgc gcgatatgga ggacatgatc cggcgcaccg
     2941 ttgggccggg ggtgcaggtc gagctgcagc tttccgatga gaaatggctg gtgatgtgcg
     3001 accctaacca gatggagagc gcgctgctca acctctgcgt caatgcgcgc gatgccatgc
     3061 ccgacggcgg ctggctgacg atcagcacgc aggagctgaa cctcacagag gccgacctgt
     3121 ccggcgtcga ggatgcaccg cccgggccct atacggttct cgttgtctcc gacaccggaa
     3181 ccggcatgcc gccggatgtg gtggcgcaca tcttcgaacc gttcttcacc accaagccga
     3241 tcggccaggg aaccgggctg ggcctgagcc agatctacgg ttttgtgcgg cagtcgggcg
     3301 gcttcgtgca ggtcgaaacg tcacccgggg cgggcaccac cgtgcgcctg ctcatgccgt
     3361 tccatgagat caatccacac agcgatcctg gcgtgctgcc cgccctgggc agaacggtgc
     3421 tgctggtcga ggacgaaccc gatgtgcgcg agctgaccgc cgagcatctc cgtgatttcg
     3481 gctaccgggt gctggaggcc gacaacgccg cggctgcgct acgcctcgtg caggccggga
     3541 cccacatcga tcttctcctc accgatttcg ggctgccagg cggcatgaac ggccgtcagc
     3601 ttgctgaagc ggtgctccag cgccacgtgg gcctgcccgt gatcgtgatc accggctacg
     3661 cggccggcga gccgatgaga gggatggagg tgctgcgtaa gccgttcgag ctggcggcat
     3721 tgaccgactt ggtgaaagcc aggctggatc catgaagcaa agtcgccatt aggctttctt
     3781 caacacaatt caccaagttc agacacaggt gtcgagccat gcggttacca cctgtcacga
     3841 aaatcgagaa aaacgcagca cgcaaaatcg aagaaacagc tgcgagaatg gaaaatgaca
     3901 ggaacggccc ccatcaggcg cgatttgacg taatgagccc ttacgcggcc agggaggcta
     3961 agtcgggcga gcccgatttc gaaagcgggc attgaggaac gatcgtatga gtggcaccgc
     4021 cgatagggcc aacgtggtca ttcaaccccc gctcgcgtgg gcgctcgcgt ttgtcgcggg
     4081 tctcgcagta gactggttct atccatcgcc gtttgtcccg acagccattc cgcgcgcctg
     4141 gatcggcggc ggcgtgttcg cggtcggtct cgcgctcgcc atttgggcaa ttgtagccat
     4201 ccggaaggca ggcacccggg ttgaagccaa caaaccgacg acggcgattg tggcggatgg
     4261 accc
//
LOCUS       DASWQN010000175         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1470069, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000175 DASWQN010000000
VERSION     DASWQN010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470069"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1361
                     /locus_tag="VGC82_02875"
     CDS             171..1361
                     /locus_tag="VGC82_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY0422244.1"
                     /translation="MASTDLPLPVAAAAWSQDSTVFVALELSRKSWLVGVSAPGDTKI
                     SKYTVAAGDGPGLLALVAKLQMKAAQRLGRPVTVVTIQEAGLDGFWLHRLLLSAGYDS
                     QVVDPASIAVDRRQRRRKTDAIDVEALLRTLMAWARGERRVCSMVHAPSPEDEDRRRP
                     SRERAALITEKTRHTNRIKGLLAGQGIFDFEPLRLAQRERLETMTTGDGRPLPPQLKA
                     EIRRHLARLDAVIRDIAAVEAERDALLAAPAITEPPTPTAADVLPAPAVLLLTLKGIG
                     PEFASVLCLEAFYRTFANRREIASYAGLTPSPWKSGRIDRDQGISKAGNPRLRTTMLE
                     LAWMWIRYQPDSVISRWFVERVGTTRGRVRRIAIVAVARKLLVALWRFVTQGLVPEGA
                     VMKA"
BASE COUNT          406 a          692 c          747 g          352 t
ORIGIN      
        1 ttcgcaggca tcatcgcctc cgataaggcc attccatact tcatcaacag ccaaaccgcc
       61 agcctcgtcc cgcaacacac ccttgacgct gcactagaga tcctatgacg gtggtgagcg
      121 gcggccggtt cggtagaatg cggttgccat tagcatcaag gaggtgaccg atggccagca
      181 ccgatcttcc ccttccggtt gcggccgccg cctggtcgca ggatagcacc gtctttgtgg
      241 cgctggaact cagccgcaag agctggctgg tgggggtcag cgcgccgggt gacaccaaga
      301 tctccaagta cacggtggcg gccggtgacg gccccgggct gctggcgctg gtggcgaagc
      361 tgcagatgaa ggccgcgcag cggctcggcc ggccggtcac ggtggtgacg atccaggaag
      421 ccggcttgga cggtttctgg ctacatcgac tgctgctgag tgccggctac gacagccagg
      481 tcgtggaccc ggcctcgatc gcggttgacc ggcgccagcg gcggcgcaag acggatgcga
      541 ttgatgtcga ggcgctgttg cgcacgctga tggcctgggc gcgtggcgag cgccgggtct
      601 gttcgatggt gcacgcgccg agcccggagg acgaggatcg ccggcggccg tcgcgcgaac
      661 gcgcggccct gatcacggag aagacccggc acaccaaccg gatcaagggt ctgctggccg
      721 gccaggggat cttcgatttc gaaccgttgc gcctggcgca gcgggagcgg ctggagacga
      781 tgacgaccgg cgacggccgc ccgttgccgc cgcagctcaa ggccgagatc cgccgtcacc
      841 tggcacggct cgatgccgtg atccgggaca tcgcggcggt cgaagccgag cgcgacgcac
      901 tgctggcggc gcccgcgatc accgaaccgc cgacaccgac cgctgccgac gtcctcccgg
      961 ccccggccgt gctcctgctg acgctcaagg gcatcggccc ggagttcgcc tcggtgctgt
     1021 gtctggaggc gttctaccgc acgttcgcca accgccgtga gattgcgtcc tatgccggcc
     1081 tgacgccgag cccgtggaaa agcggtcgca tcgaccgtga ccaggggatc tcgaaagccg
     1141 gcaatccgcg cctgcgaacg accatgctgg agctggcctg gatgtggatc cgctaccagc
     1201 cggactcggt gatcagccgt tggtttgtcg agcgcgtcgg gacgacgcgc ggccgggtgc
     1261 ggcgcattgc catcgtcgcc gtggcacgca agcttctcgt ggctctgtgg cgatttgtga
     1321 cccagggcct cgtgccggag ggcgccgtga tgaaggcctg aaccccggca cgagcgacgc
     1381 catccgcggg ccggttccgg atcggttccg cgggtccggg cgggcggtgg tcgtagcgaa
     1441 ccatggctgc aaaacgccgt ctcagagaat ggccccgccc atcccgagcc cctcgccctc
     1501 gtgcaagagg gatcatggta cgggtgatac gcacccaccg gatgtgaggt gatgcggccg
     1561 atcgggtcgc atactgtaac tcgggctcga ggcttggact tgaggactgg cagtgaagga
     1621 attcgaatga acagggcaag atcaaaatcc ccctcgccgg ggtggcatgc ctggtccgat
     1681 gaatggacca ggcaggacga tgatacggga gggctctacg tcaagagggt tccacccatc
     1741 ccagacgcca agaccgccca aatgggctcc gatgtcgccc aaggcgggct aggcgtctgg
     1801 cacgggtccc tgcgaaccct cgtgacgact gctgcagcgc cgcagcaatg aaagtattca
     1861 caaaaggctg accataggaa aggaatcccg aataggacga tgcgccacgt gacgatctgc
     1921 acacaccggc ctgcggagaa gcaggtccgg acgaatagtt acatgcaatc gaccagcgta
     1981 aaggaatgaa acgacgtact tgacagaaca caccatcatg tgaggttcgc ctgtaacatt
     2041 tttgtgcatc cgtgcaccaa agcaccggca cgctgcaatg ccggggcgac acgatcggca
     2101 tggtgaagtc ggcctacacc gtgacgcgtg atttcagcgc ctagtcgccg atggggcggg
     2161 tcggcaatgg cagggagcgc cgctggcgct gcgaacc
//
LOCUS       DASWQN010000176         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1602183, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000176 DASWQN010000000
VERSION     DASWQN010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602183"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VGC82_02880"
     CDS             complement(<1..305)
                     /locus_tag="VGC82_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0422245.1"
                     /translation="MRIAIIGAGGVGGAFGAALAKAGADVTFVARGAHLKAMRERGLR
                     IEGDRGETGIEPVRATDDPATIGPVDFVMFCVKLWDVESAGAAIKSLIGPTTAVIPL"
     gene            complement(296..943)
                     /locus_tag="VGC82_02885"
     CDS             complement(296..943)
                     /locus_tag="VGC82_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0422246.1"
                     /translation="MTPDPSRLTLYGSFTSSSSYKPMLYLALSRLPFSFRTVNLKNGV
                     QKLPGYLAINRYGQVPALRHNGLTIVQSNIVLDYLARTTGHFAGATEQDRWTAREWLS
                     WEADNITAVAKVRHYSRFRTVDPAVMAYFRPLADAAVSFVDRALEGRDWLVGDSLTIA
                     DIGCWGRMVFMAEGGMEIANWPNVHAWSRRLGALPGFALPYDLIPKKDTEFEPCA"
     gene            1293..1685
                     /locus_tag="VGC82_02890"
     CDS             1293..1685
                     /locus_tag="VGC82_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY0422247.1"
                     /translation="MKIERVALACVLGILMAFPAAAQTMQTTPGRSSSPSITTTPGPA
                     TTAPAPRGPEAPTTTRAPAPASSSALVDINSASSTDLDALPGIGKARAAAIIKNRPYK
                     GKDDLLSRHIIPPNVYKGIADKIIARQG"
     gene            complement(1760..2587)
                     /locus_tag="VGC82_02895"
     CDS             complement(1760..2587)
                     /locus_tag="VGC82_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0422248.1"
                     /translation="MSSEIARYYGKTGLLDRILAALAQAGKDIDHLTIDDLAPVDEFH
                     SRRRLATRELAAMLAPSASEQVIDIGSGLGGPSRYLAATYGCRVSGIDLTPEFVEVAT
                     ALTARTGLTGLVDFRVGSALDLPFADASFDLAWSQNVAMNIADRPRWYAEIHRVLKPG
                     GRLAIQDVVTGNGDALDFPVMWADRAEISFLRTPEDTRAMLEAAGFRVLQWVDNTAAA
                     LAEAAAERVRSAEHPPNPPVLGIHLVVGPSFREKMANGQSATATGRTRLINAVLSRP"
     gene            complement(2620..>2895)
                     /locus_tag="VGC82_02900"
     CDS             complement(2620..>2895)
                     /locus_tag="VGC82_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0422249.1"
                     /translation="EPYLRQYVRGSHGIDYKERIKIMKLLWECVGTEFGGRHELYERN
                     YAGGWEDIRAQTLMGAEKGGDMAKMEALVDQCMADYDENGWTTDTWT"
BASE COUNT          539 a          927 c          915 g          514 t
ORIGIN      
        1 tgcagcggga tcaccgcggt ggtaggaccg atcagtgact tgatggcagc cccggcggac
       61 tcgacgtccc aaagcttgac gcagaacatc acgaaatcca ccggcccgat cgtggctgga
      121 tcgtcggtgg cgcgaaccgg ctcgatgccg gtttcgccgc ggtcgccctc gatgcgcaga
      181 ccgcgttcgc gcatggcttt caaatgtgcc ccgcgggcga cgaaggtgac gtcggcgccg
      241 gctttcgcca aggcggcacc gaacgcgccg ccgacaccgc cggctccgat gatggctatg
      301 cgcatggttc gaactccgta tctttcttgg ggatcaggtc gtagggcagg gcgaagccag
      361 gcaacgcgcc caggcggcgg gaccatgcgt gtacgttggg ccagttggcg atctccatgc
      421 cgccctcggc catgaatacc atgcggcccc agcagccgat atcggcaatg gtcaggctgt
      481 cgccaaccag ccagtcgcgg ccctcaagag ccctgtccac gaagctgacc gccgcgtcgg
      541 cgagcgggcg aaaatacgcc attaccgccg ggtccaccgt ccggaagcgg ctgtaatggc
      601 ggaccttggc caccgcggtg atgttgtcgg cttcccagga aagccattca cgcgcggtcc
      661 agcgatcctg ttcggttgct ccggcgaagt ggccggtggt gcgggcgagg tagtccagga
      721 caatgttgga ctgcacaatg gtcagcccgt tgtgcctcaa ggcgggcacc tgcccgtagc
      781 ggttgatggc gagatagcca ggaagctttt gcacgccgtt cttgaggttg acggtgcgga
      841 agctgaacgg caggcgcgac agcgccagat aaagcatagg cttatacgag gaactggagg
      901 tgaagctgcc atacagtgtc aggcgggagg gatctggtgt cattgcgcgg gcagggtgct
      961 tgtgaatcgg ctggaaggca aatcgggccc agccttgcag ctgggtagcc ttgcagctgg
     1021 gcagccttgc agccggggca ttccactgag gcctgacgat cctggctccg atgttcgatc
     1081 acgccgttta acgaaagtcc catggcgttc ccgcgattca ggccgctcgg agaatgaggg
     1141 gcctgggacc ctgggaatta cttgccctca cgtcaaactt cgaggaggcg gatagaaacc
     1201 cttggtcaac ccggcctgca aatatagcat cggcagacga cgggacgctt ggccagaccg
     1261 agcgccagga cattgttgga caaaggatag ccatgaaaat cgaacgagtt gcactggcct
     1321 gcgtgcttgg gattcttatg gcgtttccgg cggcggcaca gaccatgcag acaaccccgg
     1381 ggagatcctc atcgccgtcg atcacgacaa cgccggggcc agcaaccacc gcccccgccc
     1441 ctcgcggccc cgaggcgccc accaccacgc gtgccccggc tcctgcctcg tcctcggccc
     1501 tggtggatat taactcggcg tcatcgacgg atctcgacgc gctgcccggg attgggaagg
     1561 cccgggcggc agcgatcatc aagaaccggc cctacaaggg taaggacgat ttgctgagcc
     1621 gtcatatcat tccgcccaac gtgtacaagg ggatcgccga caagatcatc gcccggcaag
     1681 ggtgaaccgc aagccattcc tgggtgccgg accaaggttc tcgaccccgg tcccgttccg
     1741 atggcaacca cgttcgcggc tacggacgcg acagcacggc attgatcagg cgggtgcgcc
     1801 cggtggcggt ggcgctctgt ccgttcgcca tcttctcgcg gaaactcggg cctacgacca
     1861 gatggatgcc gagaacgggc gggttcggcg gatgctctgc cgaccggacc cgctccgctg
     1921 ccgcttcagc caatgccgcc gcggtgttgt ccacccattg caggacccgg aagccggccg
     1981 cctccagcat ggctcgcgta tcctccggcg tccggaggaa actgatctcc gcccggtcag
     2041 cccacatcac cggaaaatcg agagcgtcgc catttcctgt tacgacgtcc tggatcgcga
     2101 gccggccacc aggcttgaga acccggtgta tttcggcata ccagcgcgga cgatcggcga
     2161 tgttcatggc gacgttctgc gaccaggcca ggtcgaagct ggcatcggca aacggcaggt
     2221 ccaacgcgga accaactcgg aaatccacca ggccggtcag gccggtgcgg gcggtcagcg
     2281 ccgtggcgac ctcgacaaac tccggcgtaa ggtcgatgcc gctgacccgg cagccgtagg
     2341 tggcagccag atagcgcgac ggcccgccga gtcccgaacc gatatcgatt acctgttcac
     2401 tggccgaggg agccagcatg gcagccagtt cccgggtcgc caggcggcgg cgggagtgaa
     2461 attcatccac cggcgccaga tcgtcgatgg tgaggtggtc gatgtccttg cctgcctgcg
     2521 caagcgcggc gagaatacgg tccagaaggc cggtcttgcc gtagtagcgg gcgatttcac
     2581 tggacatttc gttcccttta caagtagaac taccgcggct caagtccacg tatccgtcgt
     2641 ccacccgttc tcgtcgtagt cggccatgca ctgatcgacc agcgcctcca tcttcgccat
     2701 gtcgccgccc ttttccgccc ccatcagtgt ttgcgcccgt atatcctccc agccgccggc
     2761 gtaattgcgc tcatacagtt cgtgccggcc accgaattcg gtgccgacgc attcccaaag
     2821 cagtttcatg atcttgatcc gctccttgta gtcgatccca tgggagccgc gaacatattg
     2881 ccgcagatag ggttc
//
LOCUS       DASWQN010000177         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_150784, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000177 DASWQN010000000
VERSION     DASWQN010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150784"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VGC82_02905"
     CDS             <1..737
                     /locus_tag="VGC82_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422250.1"
                     /translation="PVPAMAHVKWFVPYDVTATPTPVSGVLTSHFLMLFAGFLLAVLG
                     GILFDRYIAGRWKFIVQGGNQVIIEEWLLRAGMAAFFMALFSTGGVILTPELHTDADW
                     PAWLQLGIAISLLSSRTCLIGGAGIAVLYAYGARLYGVFHLADYPMFLGIAGYLVLTS
                     LPSERLRSLRMPILYVTLCMSLMWGAIEKWAYPQWTLSLLELRPYLAAGLPHEISWCS
                     QDLWGFLLRSTSLRVSAWSGLRSLGC"
     gene            731..1033
                     /locus_tag="VGC82_02910"
     CDS             731..1033
                     /locus_tag="VGC82_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422251.1"
                     /translation="MLTIFAAAILDFGTIDAVGHLPIMAPLLVMFIHGPTRLHRWFHE
                     AGNTAFSDAHKVGVAFATSICLFFAFYYGRQYDEYGHPTHERAVASVQMSHTHQSN"
     gene            complement(1113..>1505)
                     /locus_tag="VGC82_02915"
     CDS             complement(1113..>1505)
                     /locus_tag="VGC82_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422252.1"
                     /translation="VNGMARILTAEGKGALLTMPGDIPRVTSAEIDAVVSSCLAPPSF
                     TIVPAHDGLGSNAVLCAPPFSVPLRFGGDSYFPHLITARACGIEPTIVRLPGIGLDID
                     HPADLRAFMAARPRKPTRTLALLEQFGL"
BASE COUNT          269 a          460 c          438 g          338 t
ORIGIN      
        1 ctcctgtccc cgccatggcc catgtgaaat ggtttgtgcc ttatgatgtc accgcaacgc
       61 caaccccggt cagcggggta ctgacgtcac actttcttat gctgttcgcc ggtttcctgt
      121 tggcagtact tggaggcatc ctgttcgacc gttacatcgc cggcaggtgg aagttcatcg
      181 tgcagggagg taaccaagtg attatagaag aatggctgct gcgcgccgga atggccgcct
      241 ttttcatggc gctcttctcc accgggggcg ttattctgac accggagttg cacacggatg
      301 cggactggcc cgcttggctg caactgggta tcgcgatcag cttattgtcc agccgtacat
      361 gcctcatcgg cggagccggc atcgcagtac tttacgcgta tggggcacgg ctttatggcg
      421 ttttccatct tgcggactat ccgatgttcc tgggcatagc cggctatttg gttctcacat
      481 ccttaccatc ggagcgcctg cgctcgctgc gcatgccgat cctgtacgtc acgctgtgca
      541 tgagcctgat gtggggcgcg atcgagaaat gggcgtatcc gcaatggacg ttgtcgctgc
      601 tggagttgcg cccgtacctg gcggccggcc tgccgcacga gatttcctgg tgttcgcagg
      661 atttgtgggg ttttctcttg cgttctacat ccttgcgggt ttcagcttgg tccggcctgc
      721 gatccttggg ttgctgacga tattcgccgc agccatcttg gacttcggca caatcgatgc
      781 tgttggacat ctgcctatca tggcgccgct gctggtgatg ttcatccatg ggcccacccg
      841 gctgcaccgc tggtttcatg aagcgggcaa tactgccttc agcgatgcgc acaaagtagg
      901 cgtcgcgttc gctacctcga tctgtctgtt ctttgccttc tactatgggc gtcagtatga
      961 cgaatatggc cacccaaccc acgaacgggc agttgcgtcc gtacagatgt ctcacacgca
     1021 tcagagcaat tgatcgaatg agccacgcgc tgctgagctt ccgcggtgcg atcacgctca
     1081 agagccagtc gccacgacca caatcgccgt cgctacagcc cgaactgctc cagcaatgcc
     1141 agcgtccgtg tcggctttct tggccgagct gccatgaaag cccgcagatc tgccgggtga
     1201 tcgatgtcca acccgatccc cggcagccgc acgatggtcg gctcgatccc gcacgcccgg
     1261 gcagttatca aatgcgggaa gtaactatcg ccgccgaagc gaaggggaac agaaaacggc
     1321 ggcgcgcaaa ggaccgcatt cgacccaagt ccgtcgtggg ccggcacgat ggtgaacgac
     1381 ggcggcgcca gacaggacga aaccaccgca tcgatctccg ccgaggttac ccggggaatg
     1441 tcgcccggca tcgtcagcaa ggcgcccttc ccttcggcgg tcagtatccg cgccatgcca
     1501 ttgac
//
LOCUS       DASWQN010000178         3580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2525948, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000178 DASWQN010000000
VERSION     DASWQN010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2525948"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VGC82_02920"
     CDS             complement(<1..222)
                     /locus_tag="VGC82_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0422253.1"
                     /translation="MLRVAATVEQALDVLLPEPEGAEARLAEAMRYATLGGGKRLRAF
                     LVMQSASLFGVNETCAARVAASVEMLHAYS"
     gene            599..1525
                     /gene="ispH"
                     /locus_tag="VGC82_02925"
                     /gene_synonym="lytB"
     CDS             599..1525
                     /gene="ispH"
                     /locus_tag="VGC82_02925"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY0422254.1"
                     /translation="MRVVLAQPRGFCAGVERAIEIVERALKKYGPPIYVRHEIVHNRH
                     VVESLRARGAVFVDELHEIPPGSMTVFSAHGVAKRVEDAAAERGLPVIDATCPLVAKV
                     HNEGRRYARQDREIVLVGHAGHAEVEGTIGQIPAKVHLVQTVADVALLQVKDPEKLAY
                     ITQTTLSVDDTRGIISALTARFPSIVGPDVRDICYATQNRQQAVRDLAHQVDMILVVG
                     SRNSSNSNRLCEIGQELGKPSYLIDDADALQGAWFEGIASVGVTAGASAPETLVQGVL
                     EGLRRFGVIEISTQDGVAEDVRFRFPAQLADA"
     gene            1538..2698
                     /gene="hpnH"
                     /locus_tag="VGC82_02930"
     CDS             1538..2698
                     /gene="hpnH"
                     /locus_tag="VGC82_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEY0422255.1"
                     /translation="MTVPLNQALRVGAYVVKQHLIGRKRYPLVLMLEPLFRCNLACSG
                     CGKIDYPAEILNQRLSVAECMEAVEECGAPVVVIAGGEPLLHKEIDQIVQGAIDRKKF
                     VIVCTNALLLEKKMDLFKPSKWFSWSVHLDGGKTEHDISVCQEGVYDRAVAAIAESKR
                     RGFRTIINATLFNNAEPAKVAAFFDDVMAMGVDGISVSPGYAYERAPDQQHFLNRAKT
                     KELFRGIFSRQGKGQGKKKWSFNQSSMFLDFLAGNQTFHCTPWGNPTRTYFGWQRPCY
                     LLGEGYAKSFKELMETTDWDKYGTGNYEKCADCMVHSGYEASAVAETVRRPWRAAAQA
                     IRGIRTDGPMAPDIPLDRQRPAEYVFSRHVETAVKRIRAGKKPADEVADAAD"
     gene            2815..3300
                     /locus_tag="VGC82_02935"
     CDS             2815..3300
                     /locus_tag="VGC82_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY0422256.1"
                     /translation="MWFTADTHFGHGGALALYRRPFASVAAMDAAMVARWNETVQADD
                     EVWHLGDFALRTGAAACAVLLRSLNGRKHLVTGNNDPPEIAGLSEWASVQAYAEITVD
                     AHRLVLCHYAFRTWNGMAKGALNLHGHSHGRLKKLPRQADVGVDCSGFRPIALHQLHG
                     A"
BASE COUNT          667 a         1082 c         1149 g          682 t
ORIGIN      
        1 cgaataagca tgcaacatct ccactgatgc agccacgcgc gcggcgcatg tctcgttcac
       61 gccgaacaac gaggcgcttt gcatcacgag gaaggcccgc aggcgcttgc caccgcccaa
      121 cgtggcgtag cgcatggctt ccgccagtcg ggcttcggcg ccctctggtt cgggcaacag
      181 cacgtccagc gcctgctcca cggttgcagc cacgcgcagc atttcgtcgc cgagttcgct
      241 tgaagagaga aaatctgcag acgccaaatt cagccccgtg aactgcgact ccgccaaacg
      301 agagccgatt ttttagcgga taacacttcc agggagtttt cgcatggtaa cggccgcatg
      361 atcgacgcga ctgttgccct tttgccacgc ttgtgccggg ttacgcgttg tcgtggcccg
      421 gatggccggg gtggcagcag atgaggcacg acgaagcctt gaatcgctcg taatccccgc
      481 gattatcaac cgtggagaca gattttgcgg cgttgcagca agtcgcccga cctgccatat
      541 cgcccgtccg gtccggtctc acgtcggaaa ccgcttgcat gaggaagctg gctgcatgat
      601 tcgcgtcgtc cttgcccagc cccgcggctt ttgcgccgga gtggaacgtg ccatcgagat
      661 cgtcgaacgc gcgctcaaga agtacgggcc gccgatctat gtccgtcacg agatcgtgca
      721 taaccgccat gtggtagaga gccttcgggc ccgcggcgcc gtgttcgtgg atgaactgca
      781 tgaaattccg cccggatcga tgaccgtgtt ttccgcccac ggcgtggcga agcgggtcga
      841 ggatgccgcc gcggaacgtg ggctgccggt gatcgatgcc acctgcccgc tggttgcaaa
      901 ggtgcataac gagggccgtc gctacgcccg ccaggacagg gaaatcgtgc tggtcggtca
      961 tgccggtcat gccgaagtgg aaggcaccat aggccagata ccggccaaag tgcatttggt
     1021 gcagaccgtc gccgacgtag cgctgctgca ggtgaaggat cctgagaagc tggcctacat
     1081 cactcaaacg acgctgtcgg tcgatgacac gcgcgggatc atctcggcgc tgacggcccg
     1141 cttcccgtcc atcgtcgggc cggatgtgcg cgacatctgc tatgccacgc agaaccggca
     1201 gcaggcggtg cgcgacctgg cgcatcaggt ggacatgatc ctggtggtcg gttcgcggaa
     1261 ttcatccaac tccaaccgcc tgtgcgagat cgggcaggaa ctcggcaagc cgtcctacct
     1321 gatcgacgac gccgatgccc tgcagggcgc atggttcgag gggatcgcct cggtcggtgt
     1381 caccgccggg gcttcggcac ccgaaacgct ggtgcaaggc gtgctggagg gcctgcgccg
     1441 gttcggcgtg atcgaaattt ctacccagga tggggtggcc gaggacgtcc ggttccgctt
     1501 tcccgcccaa ctcgcggacg cataaggaaa tcttactatg accgttccgt tgaaccaggc
     1561 gctgcgggtc ggcgcttatg tcgtcaagca gcatctgatc ggccgcaagc gctatcctct
     1621 ggtgctgatg ctggagccat tgtttcgctg caacctagct tgctccggat gcggcaaaat
     1681 tgattatccc gcggaaatcc tgaaccagcg cctgtcggtc gcggaatgta tggaagcggt
     1741 cgaggaatgc ggcgccccgg tggtggtgat cgccggcggc gagccgctgc tgcacaagga
     1801 gatcgaccag atcgtgcagg gtgcgatcga ccgcaagaag ttcgtcatcg tttgcacgaa
     1861 cgcgctgctg ctcgaaaaga agatggatct gttcaagccg tcgaaatggt tctcgtggtc
     1921 ggtgcatctg gatggcggca aaaccgaaca cgatatttcg gtgtgccagg aaggcgtgta
     1981 cgatcgggcg gtggcggcga tcgccgaatc caagcgccgc ggctttcgca ccatcatcaa
     2041 cgcgacactc ttcaacaatg ccgaaccggc caaggtcgct gcgttcttcg acgacgtcat
     2101 ggcgatgggc gtggacggga tttcagtctc ccccggctac gcctacgaac gggcgccgga
     2161 ccagcagcat ttcctgaacc gggcaaagac caaggagttg ttccgcggta ttttcagccg
     2221 gcaaggcaaa ggccagggga agaagaagtg gtcgttcaac cagtcctcga tgttcctgga
     2281 tttcctcgcg ggcaaccaga cgttccactg cacgccgtgg ggcaacccga cccgcaccta
     2341 ttttggctgg cagcgtccct gctacctgct gggtgagggt tatgcgaagt cgttcaagga
     2401 gttgatggaa acgacggact gggacaagta cggcaccggc aactacgaaa agtgcgccga
     2461 ctgcatggtc cattcgggct acgaggccag cgccgttgcg gaaacggttc gccgtccatg
     2521 gcgggcggcg gcgcaggcta ttcgcggcat ccgcaccgat ggcccgatgg cgccggacat
     2581 cccgctggac cggcaacgtc ccgccgaata cgtgttcagc cgccatgtcg aaacggcggt
     2641 gaagcgcatc cgtgccggga agaagccggc tgacgaagta gctgacgccg cggattgagc
     2701 gatgagcgcc cgccgcgtcc ctggtccggg cggaacggtc cttgtacaag ggccgaacga
     2761 tgcgggatct ttccgccgcg ccttcaggct ggatagggcg tagccgaatt ggctgtgtgg
     2821 ttcacggctg atacccattt cggccatggc ggtgcgcttg cgctttatcg ccggcccttc
     2881 gcttccgtgg ccgctatgga tgccgcgatg gtcgcgcggt ggaacgaaac tgtccaggcg
     2941 gatgatgaag tctggcacct cggcgatttt gcgctgcgaa ccggcgcagc tgcttgcgcc
     3001 gtgttgctgc gatcattgaa cggacgcaag cacctggtca ccggcaacaa cgatccgccc
     3061 gaaatcgccg ggctgagcga atgggcgagc gtgcaggcct atgccgaaat cacggtcgat
     3121 gcgcacaggt tggtgctgtg ccactatgcg tttcgcacat ggaacggcat ggcaaagggg
     3181 gcgctcaacc tgcatggcca tagccatgga cgattgaaga agctgcctcg gcaggcggat
     3241 gtcggtgtcg attgttcggg gttccggccg atcgcgctgc atcagttgca cggcgcgtga
     3301 tgttcgggtg ccggtttggc gcggccaacc acgacttccc ttgcagccgc gagagaccgt
     3361 gtttccagtc accgtgaacc gctccagtgt cccgaatctg aagtccgccg tatccggcga
     3421 acctcagtct ggcgccactc tctttgtttc agtggcctgc tgacccccct gcgtccggcg
     3481 catgatgcga ccaacttcgg gggcaggaca ctactagtca cagggacatc cgtccgttgg
     3541 acccgatgcc ttgcgaatca ggtgatggtc gatccactcc
//
LOCUS       DASWQN010000179         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_678534, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000179 DASWQN010000000
VERSION     DASWQN010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678534"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VGC82_02940"
     CDS             complement(<1..474)
                     /locus_tag="VGC82_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEY0422257.1"
                     /translation="MENSQVNDMVAPIDYATAKPGGSSAKPRGPSAEFKGSGALTPMA
                     KNGSAPSTAEATPGCSDAVRRAKPARVPTECGDLPFLIKRDGTWLYRGSPINRKELVC
                     LFSSVLRREEDGSWWLQTPAERGRIDVEDAPFIAVELDWTGDGRHQVLSFRTNIDE"
     gene            632..1618
                     /locus_tag="VGC82_02945"
     CDS             632..1618
                     /locus_tag="VGC82_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0422258.1"
                     /translation="MSDDPNAILSATEALGARIVQVRAAIGRVIFGQDEVIDQTLITL
                     LSGGHVLLVGVPGLGKSRLVETLGIVLGMATKRIQFTPDLMPADIVGSEVLDETNGRR
                     AFRFVPGPVFCQLLMADEINRASPRTQSALLQAMQEQRVAIGGVEHALPRPFHVLATQ
                     NPIEQEGTYPLPEAQLDRFLLEVNVTYPDLDAERTMLLATTGTQEAKPAQAMTAEDLL
                     TAQALVRRLPVGEKVVDAILGLVRGCRPESAGDDIVRRHVAWGPGPRAAQALMLATRA
                     RALLGGRLAPSVDDVAALARPVLRHRMALSFSARADGIALENVIDRLVGNIG"
     gene            1635..>1855
                     /locus_tag="VGC82_02950"
     CDS             1635..>1855
                     /locus_tag="VGC82_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422259.1"
                     /translation="MPDHALWQHGLVMIGQGVGVGHGVGGHLVTDGGIMCPELPATAW
                     SVPNRLWPALSRQSTTSRHVGAIAGSRAK"
BASE COUNT          325 a          574 c          609 g          347 t
ORIGIN      
        1 ttcgtcgatg ttggtgcgaa aggacagcac ctgatgccgc ccatcgccgg tccaatcgag
       61 ttcgaccgcg atgaacggtg catcctcgac gtcaattcgg ccgcgttcgg ccggggtctg
      121 cagccaccaa ctgccgtcct cttcacggcg gagcacactg gaaaacaaac agaccagttc
      181 ttttcggttg atcgggctgc ctcgataaag ccacgttcca tcgcgcttga taaggaaggg
      241 gaggtcgccg cattcggttg ggacccgtgc aggctttgcc cgacgcaccg catccgaaca
      301 ccccggcgtc gcctcggcgg tcgatggggc ggagccattc ttggccatcg gcgtcagcgc
      361 acccgaaccc ttgaactcgg cggaaggtcc gcgaggcttt gctgaagatc cgccaggctt
      421 cgcggtggcg taatcgatgg gcgctaccat gtcgttcacc tgactgtttt ccatggaggc
      481 cgaacttcct aacacgaccg cgatataggt accatccccc cgctcatgaa agagcctata
      541 tgcttgcaga cgcacaaacg cgaaggatga tccccgtatg tcgggcggta tggctggcac
      601 gccacagggt ttttcgagcc ggatcagtcc gatgagcgac gatccaaacg caatcctttc
      661 cgcgaccgag gcgctaggag cccggatcgt gcaagtccgc gcggcgatcg gccgggtgat
      721 cttcggtcag gatgaggtga tagatcagac tttgatcaca ttgttgtcgg gtggtcacgt
      781 tctgctcgtc ggggtgcccg gcctgggcaa gagccgcctg gtggaaacgc tgggaatcgt
      841 cctgggcatg gccaccaagc gcattcagtt caccccggac ctgatgccgg ccgatatcgt
      901 cggcagcgag gtgctggacg aaaccaatgg acggcgcgcg tttcgcttcg tccctggccc
      961 ggtgttctgc caattgctga tggccgacga gatcaaccgc gccagccctc gcacgcagtc
     1021 cgcgctgttg caggccatgc aggaacagcg cgtcgctatc ggcggcgtgg agcatgcgct
     1081 gcctcgtccg ttccatgtcc tggccacgca gaatccgatc gagcaggaag gcacctatcc
     1141 gctgccggaa gcgcaactcg accgcttcct gctggaggtc aacgtgacct accccgacct
     1201 cgatgcggaa cgcaccatgc tgctggcgac caccgggacg caggaggcaa agcctgccca
     1261 ggccatgacc gcggaggacc tgctgaccgc acaggcgctg gttcgccgct tgccggtggg
     1321 cgagaaggtc gtcgatgcga tccttggcct ggtgcgtggc tgccggccgg aaagtgccgg
     1381 cgacgatatc gtccgccggc acgtcgcctg gggtcccggt ccgcgtgccg cacaggcctt
     1441 gatgctggcg acgcgggcca gggcgctgct aggaggacga ttggcaccaa gcgtggacga
     1501 tgtggcggcg ctggcacgtc cggtattgcg gcatcggatg gcgctaagtt tttcggcccg
     1561 tgcggacgga atagcgctgg aaaatgtgat cgaccgattg gttggcaata ttgggtaggt
     1621 tttccctgag ccgcatgccc gaccacgccc tgtggcaaca cggccttgtg atgatcgggc
     1681 aaggtgtcgg cgtcgggcac ggtgtcggcg ggcacctcgt gacggacggt ggcatcatgt
     1741 gcccggagtt gccggctacc gcttggtcgg tgccgaatcg gttatggccg gccctgtccc
     1801 ggcaatccac gacttcccgg catgtaggtg cgatcgcagg tagccgcgcc aagca
//
LOCUS       DASWQN010000180         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_546955, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000180 DASWQN010000000
VERSION     DASWQN010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546955"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1283)
                     /locus_tag="VGC82_02955"
     CDS             complement(3..1283)
                     /locus_tag="VGC82_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEY0422260.1"
                     /translation="MAQTNPTAQTSPTALTNQRIDGKRLWDSLMGFAEIGATPKGGVR
                     RLTLTDVDKRGRDRFRTECEALGLTVRVDAIGNMFARRPGRDPSRAPVLFGSHLDSQP
                     SGGKFDGALGVLAGLEVMRSLNDLGIATEAPLELINWTDEEGSRFGHSLMGSGVWAGV
                     FGLEKAYGLTDTEGVTVERALDQIGYRGPEPASPFNADAYFELHIEQGPILEREARQI
                     GIVTGAQSQVWYDAVTIGQDSHAGTTPPSTRKDALVCAARIIDLVDRMMRARGEDGRG
                     TVGQLLVSPNSRNVVPGEVRFSVEFRHPTDAEVDVLDKEFPPAAQAIADACGVKLELT
                     TLFRIPAQPFDAGCVDLVRQGAAKLGYSTRDIVSGAGHDAVYAARHVPTAMIFTPCKD
                     GLSHNEAESIEPEEAEAGCQVLFEAVVARANRSL"
     gene            1331..>1896
                     /locus_tag="VGC82_02960"
     CDS             1331..>1896
                     /locus_tag="VGC82_02960"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEY0422261.1"
                     /translation="MTAPSILHYADLDSPNVWQARVDLAACFRTAARMGLHEGVCNHL
                     SFMVPNRDDLFLVNPDGWSFAEITASRLLICDFHRNVIAGDGVPEDTAFFIHARLHAR
                     IPRARAAFHTHMPNATALTMVEGDPLVWAGQTALKFYGRTAVDDAYSGLALDESEGDR
                     IAAAAGDADIVFMKHHGVMVLAPTIAEA"
BASE COUNT          294 a          659 c          596 g          347 t
ORIGIN      
        1 gtttataaac ttctatttgc ccgagcgacc acagcttcaa agagcacctg gcatccggcc
       61 tcggcttcct cgggctcgat gctttcggct tcgttgtgcg acaggccatc cttgcacggg
      121 gtgaagatca tcgccgtcgg cacatgccga gcggcataga ccgcgtcatg cccggcgccg
      181 gaaacgatgt cgcgggtgct gtagcccagc ttggccgcgc cctgccgcac cagatcgaca
      241 catcccgcgt caaacggctg ggccgggatc ctgaacagcg tggtcaactc cagcttcacc
      301 ccgcaggcgt cggcgatcgc ctgggccgcc ggcgggaact ccttgtccag aacgtcaact
      361 tcggcatccg tcggatgccg gaattcgacc gagaaccgca cctcgcccgg caccacgttc
      421 cggctgttcg gggaaaccag aagctgcccg accgtgccgc gcccatcctc gccgcgggct
      481 cgcatcatgc ggtccaccag atcgatgatc cgcgccgcgc aaaccaatgc gtccttgcgg
      541 gtcgaaggcg gtgtggtgcc ggcgtggctg tcctgcccaa tcgtcaccgc gtcgtaccag
      601 acctgggact gagcgccggt cacgatcccg atctggcggg cctcgcgctc caggatcggg
      661 ccttgctcga tatgcagttc gaagtaggcg tcggcgttga acggagaagc gggttccggc
      721 ccgcgatagc cgatttggtc caacgcgcgc tcgaccgtta cgccttcggt gtccgtcagc
      781 ccatacgcct tctccagccc gaagaccccg gcccagacgc ctgatcccat caggctgtgg
      841 ccgaaccggc tgccttcctc gtccgtccag ttgatcagtt ccagcggggc ctcggtggcg
      901 atgccaaggt cgttcaggct gcgcatcacc tccagcccgg cgagcacacc cagcgcccca
      961 tcgaactttc cgccgctggg ctgactgtcc aggtggctgc cgaacagcac cggggcgcgg
     1021 gatgggtcgc gccccggccg ccgcgcgaac atgttgccga tcgcgtccac ccgcacggtc
     1081 aggcctagcg cctcgcactc ggtgcggaac ctgtcgcgtc cgcgcttgtc cacgtcggtc
     1141 aacgtcaggc ggcgcacgcc gcccttgggg gtggcgccga tctcggcgaa gcccatcagg
     1201 ctgtcccaga ggcgtttgcc gtcgatacgc tggtttgtca gggcggtcgg acttgtctgg
     1261 gcggtggggt tcgtctgtgc catgacgcag catcttgccc agcggccggc ttcctgcaag
     1321 cctggatcgc atgaccgcac catcgattct gcattatgcc gatctggata gcccgaatgt
     1381 gtggcaggcg cgggtggatc tcgcggcttg cttccgaact gccgcgcgga tgggcctgca
     1441 cgagggcgtc tgcaatcacc tgtcctttat ggttccgaac cgcgacgacc tgttcctggt
     1501 caaccccgac ggctggtcgt tcgccgaaat aacggcttcc cgcctgctga tctgcgactt
     1561 tcatcgcaac gtcatcgccg gcgacggtgt gccggaggac accgcgtttt tcatccacgc
     1621 ccggctgcat gctcgcattc cgcgcgccag ggccgccttc cacacgcaca tgccgaatgc
     1681 caccgcgctg accatggtgg agggcgatcc gctggtctgg gccggccaga ccgccttgaa
     1741 gttctacggc cgtaccgcgg tggatgacgc ctacagcggg ttggcactgg acgagtcaga
     1801 gggcgaccgg attgccgcag ccgccggaga cgccgacatc gtgttcatga agcatcacgg
     1861 cgtcatggtg ctggccccga ccatcgcgga agcctg
//
LOCUS       DASWQN010000181         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1866758, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000181 DASWQN010000000
VERSION     DASWQN010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866758"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VGC82_02965"
     CDS             <1..537
                     /locus_tag="VGC82_02965"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0422262.1"
                     /translation="NSCFGKNAQELRWEFDGFTPTAMTGGTLEHSAIERNLIFALTGR
                     LHARRYQAYTSKLKIPISGSVRYPDPFVACSPLPRGTLVVTDPVVVFEVLSPSTASTD
                     IGAKNEECRDTPSIQRYVMLARDRQSVTVFARAGDDWVGHIVSGDAVLAMPEIGFEIP
                     IAKLYEGVPFEPAMPATG"
     gene            complement(527..1024)
                     /locus_tag="VGC82_02970"
     CDS             complement(527..1024)
                     /locus_tag="VGC82_02970"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY0422263.1"
                     /translation="MTWSDDDKTWMRRAIALAREGEVGPGKNPIGCVIVRDGEVIGEG
                     SNEVGQRHDATAHAEIVSMRRAGERLGCEEMRGAVLYTTLQPCGMCTMASIWAKIGRI
                     VYGAGRGDVHQMYFEDRHLDTADFIQDAFRDDLSIDGGLMAKECAALYVPPEADVPKN
                     EQFNR"
     gene            1139..>1683
                     /locus_tag="VGC82_02975"
     CDS             1139..>1683
                     /locus_tag="VGC82_02975"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0422264.1"
                     /translation="MEIRRLTGHTGAEVMGIDLTQPISAATQHALNRAFAEHSVLVIR
                     DQRLTPPQVLTAVELFGPVFQQHNTRFALPECPQIHYISNQDHFPDGTRYIPGEGWHT
                     DHSNDARPPKATVLHAVTLPEQGGDTQFANMAAAYAALPSAMQDRLANLIAIHVYQSS
                     HSARRLMSLSDVNKERVPNAVL"
BASE COUNT          325 a          564 c          492 g          302 t
ORIGIN      
        1 aattcgtgct ttgggaaaaa cgcacaggaa ctacgttggg aatttgatgg tttcaccccg
       61 acggcgatga ccggcggcac cttggaacat tccgctatcg agcggaactt gatattcgcg
      121 cttaccggtc gcctccacgc aagacgctat caggcttata ccagcaagct caaaatcccg
      181 atttcggggt cagtccgcta tccggacccg ttcgtcgcct gctcgccgct gccgcgcgga
      241 acgctggtgg tgaccgatcc ggtcgtcgtg ttcgaagtgc tcagccccag caccgcgagc
      301 acggacatcg gcgccaagaa cgaggagtgc cgcgatacgc cctcgatcca gcgctacgtc
      361 atgttggcgc gggaccggca aagcgtgaca gtatttgccc gcgccggcga tgactgggtt
      421 gggcatatcg tttcaggcga tgcagtcctg gccatgccgg aaattggatt cgagatcccc
      481 atcgcgaagc tatacgaagg cgtgcccttc gaaccagcca tgcccgctac cggttgaact
      541 gctcgttctt cggcacgtcc gcctcgggcg gcacgtaaag cgcggcgcac tccttcgcca
      601 ttagtccgcc gtcgatcgac aagtcgtcgc gaaaagcatc ctggatgaag tcggcggtgt
      661 ccaggtgccg atcctcgaaa tacatctgat ggacatcgcc gcgcccggcg ccataaacga
      721 tccgtccaat cttcgcccag atcgacgcca tggtgcacat gccgcagggc tgcaatgtcg
      781 tgtacagcac cgcgccgcgc atttcctcgc accccagccg ctcgcccgct cggcgcattg
      841 aaacgatttc cgcgtgtgcg gtcgcgtcgt ggcgctgacc gacttcattg ctgccttcgc
      901 cgatcacctc gccatcccgg acgatgacgc acccgatcgg gttcttgccg gggcccacct
      961 caccttccct cgccagggcg attgctcgcc gcatccaggt cttgtcgtcg tcgctccagg
     1021 tcatgacggc acctccaggg acacgcatca aggttgccgg cgacaggatt caaggccggc
     1081 aaccgtgacg tgaaccgccg gccggtttcc gctcgcggca aatcaggagg atatcgccat
     1141 ggaaatcagg cgtttgaccg gccataccgg cgcggaagtt atggggatcg acctgaccca
     1201 gccgatttcc gcggcaacgc agcacgcgct gaaccgagca ttcgcggagc atagcgtgct
     1261 ggtgatcagg gatcagaggc tgactccgcc acaggttctg accgcggtgg aactgttcgg
     1321 gccagtcttc caacagcaca acacgcggtt cgcgctgccc gagtgcccgc aaatccatta
     1381 catctccaac caggaccact ttcccgacgg cacacgctac atcccgggcg agggctggca
     1441 caccgatcac tccaatgacg cgaggccgcc aaaggccacc gtgctccatg cggtgacgct
     1501 gcccgagcaa ggcggcgaca cgcagttcgc caacatggcc gcggcctatg ccgcgctgcc
     1561 gtcggcgatg caggaccggc tggccaacct gatcgcgatc cacgtctatc aaagcagcca
     1621 tagcgcccga cgattgatga gcctgtcgga cgtcaacaag gagcgggtgc cgaacgccgt
     1681 gct
//
LOCUS       DASWQN010000182         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_678815, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000182 DASWQN010000000
VERSION     DASWQN010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678815"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..707)
                     /gene="mlaD"
                     /locus_tag="VGC82_02980"
     CDS             complement(132..707)
                     /gene="mlaD"
                     /locus_tag="VGC82_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEY0422265.1"
                     /translation="MGRHGVAEILTGALVLAIALGFLAYAVAHSGRTTGTGYSILGRF
                     DHIDGLAVGGDVRIAGVKVGTVTDERIDPKTFSAIVTMTVREDIHLPKDTGASITSES
                     LLGGKYISLSPGGDETDLKPGESVAVTQSSVSLEELLGKFIFSATSLNSPKDGKTGGG
                     NQSGSDSGGGSGGSGSGGAGGSGGGLPPLSK"
     gene            complement(715..1068)
                     /locus_tag="VGC82_02985"
     CDS             complement(715..1068)
                     /locus_tag="VGC82_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEY0422266.1"
                     /translation="MNIGTWLFTKLKGRQIGSDAFGNLYYEEKRQRPGSLRNRRWVAY
                     AGAPEASAVPPEWHAWLHYTTDQPLSDTGRKVWQKPHVANATGTPAGYRPPGHDYEGG
                     NRARASGDYEAWTPS"
     gene            1247..>2077
                     /locus_tag="VGC82_02990"
     CDS             1247..>2077
                     /locus_tag="VGC82_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HEY0422267.1"
                     /translation="MGMKTSRITPHPIWHGVRMRRTWASPDPDTAPRLVTLPASWDDV
                     AAEALSALAPATGPITLVAAAQAWIAPVAAAATAFGFDIPLADRLHRLLLLRRGAPTE
                     AIWRQEAADAPGFVLNLPAFLDNDGQLDLEAFAEAVETAVIALSVAAPLARDMQVGMA
                     DLAGLLATLGIDYGSDAARDVARCLAAILRGRADAASARWGRQTAGMGPAALDWPPPP
                     AQAAIRGLSEAANAARQAAAEMEARHHRATTCIARPGLAEALLGCETGGVAPTYAPID
                     N"
BASE COUNT          332 a          754 c          636 g          355 t
ORIGIN      
        1 cacggcgtcc tcgaagacat cccgcagcac ctcggacacc tcctggtaat tctccgacag
       61 catcttgagt ttctcccggc accccacggc gttgccgtcc aggccgggcc agttggtggg
      121 tgccgcgggg atcacttcga caacggcggc aatccgccgc cactcccgcc ggcgccccca
      181 ctcccgctgc cgccactccc gccgccacta tcactcccgg attgattgcc tccccccgtc
      241 ttaccgtcct tcggactgtt caggctggta gcgctgaaga taaacttgcc cagaagctcc
      301 tcgaggctga ccgaggactg ggtgaccgcg accgattccc cgggcttcag gtcggtttca
      361 tcgccgcccg gcgacagcga gatatacttg ccgcccagca ggctctcgct ggtgatcgag
      421 gcgccagtat ccttcggcag gtggatatcc tctcgcaccg tcatcgtaac gatcgcgctg
      481 aacgttttcg gatcgatccg ctcatcggtt acggtgccga ccttcacccc ggcgatgcgg
      541 acgtcgccgc cgacggccag accgtcgatg tggtcgaagc gccccagtat cgagtaaccg
      601 gtcccggtcg tgcgcccgga atgcgccacc gcataagcca gaaagcccag tgcgatcgcc
      661 agcacgagcg cacctgtcag gatctccgca acgccatgcc ggcccatatc gatgctaact
      721 cggcgtccag gcttcgtagt cgcccgatgc ccgggcgcgg ttcccacctt cgtagtcatg
      781 gccgggtggc cgatagccgg cgggcgttcc cgtggcgttc gccacatgcg gcttttgcca
      841 gaccttgcgg cccgtgtcgg acagcggctg gtcggtcgtg taatggagcc acgcatgcca
      901 ctccggcgga accgcactgg cttccggcgc gccggcgtaa gcgacccaac gccggttgcg
      961 caagctgccc ggccgctgcc gcttttcctc gtaataaagg ttgccgaagg catcgctgcc
     1021 gatctggcga cctttgagtt tggtaaacag ccatgtgcca atattcatgg cccaacatat
     1081 acgcagccct catggtttgg tccacccacc gcaacgcgag gaccggccac gggttcgaca
     1141 ccgcgggacg ctttcactcg gcgcgagtta tccacaacat tttgcgtgcc ccggcgagtt
     1201 tctccacaaa ataagctatc aggacggttc gccgtcccat aacatcatgg gcatgaagac
     1261 ctcgcggata acgccccacc cgatctggca tggcgtgcgg atgcgccgaa cctgggcctc
     1321 gcccgatccg gataccgcgc cgcgcctggt taccttgccc gcctcctggg acgacgtcgc
     1381 agcggaagca ttgtcagccc tggcgccggc aaccggtccg atcaccctcg ttgccgcggc
     1441 ccaggcctgg atcgcgccgg tcgccgccgc ggcaaccgcc ttcgggttcg acatcccgtt
     1501 ggcggatcgg ctgcaccggt tgctgctgct gcgccgaggt gccccgaccg aggccatctg
     1561 gcgccaggaa gccgcggacg cgccgggatt cgtgctcaac ctgccggcgt tcctggacaa
     1621 cgatggccaa cttgatctcg aagcgttcgc cgaggcggtc gaaaccgcgg tcatcgccct
     1681 gagcgtcgcc gcacccctgg cgcgcgacat gcaggtcggc atggccgacc ttgccggact
     1741 gctggcgacg ctggggatcg actacgggtc ggacgcggcc cgcgacgtcg cccggtgcct
     1801 cgccgccatt ctgcgcggcc gagcggatgc ggcttcggca cgctggggac gacagaccgc
     1861 aggcatgggt ccggcggcgc tcgactggcc gccacccccg gcccaggcgg cgatacgagg
     1921 cctgtcggaa gccgccaacg cggcccgtca agccgccgcg gagatggaag ccaggcatca
     1981 tcgagccacg acctgcatcg ctcgtcccgg attggccgag gcccttctcg gttgcgaaac
     2041 cggtggcgtc gcgcccactt acgccccgat cgacaac
//
LOCUS       DASWQN010000183         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_678848, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000183 DASWQN010000000
VERSION     DASWQN010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678848"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VGC82_02995"
     CDS             <1..386
                     /locus_tag="VGC82_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422268.1"
                     /translation="FAGAFFATRQAFISPESFTFIESATVLAIVVLGGLGSQLGVALA
                     AIVMVGGLEALRDFLSFLGDQMNSPVFAGIASELPLYRMLIFGLALVVMMVLRPRGLV
                     SGRTPTVALTRIKEIAPEIVAQGRG"
     gene            386..1186
                     /locus_tag="VGC82_03000"
     CDS             386..1186
                     /locus_tag="VGC82_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0422269.1"
                     /translation="MLSVTNLTMRFGGLRAVDDLSFEARAGDITAVIGPNGAGKTTVF
                     NCITGFYRPTGGTVVLTHPDGTTYPLQRMQGHRIAGLAKVARTFQNIRLFAGMTVLEN
                     LLVAQHNTLMAATGFGVLAVLGLGRYRAAEAAGMEKARFWLDAINLTARADDPAGALP
                     YGAQRRLEIARSMCIDPVLLCLDEPAAGLNPRESEELNRLLRRIRDQGCSLLLIEHDM
                     GVVMRISDHIVVLDHGKKISDGTPAEVRADPAVIAAYLGEGEEDEVPA"
     gene            1192..1905
                     /locus_tag="VGC82_03005"
     CDS             1192..1905
                     /locus_tag="VGC82_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0422270.1"
                     /translation="MLSLSGVTAFYGAIQALRGIDLAVEQGEIVTLIGANGAGKSTLM
                     MTICGNPRARSGTIRHQSQDITQWPTHEIMRRGIAQAPEGRRIFGRMTVRENLLMGAE
                     VAGRRDIPELLDQVFTLFPRLQERIAQRGGTLSGGEQQMLAIGRALMSRPTLLLLDEP
                     SLGLAPLVVRQIFAAIRELNRSTGLTVLLVEQNAYQALRLAHRGYVLVNGSITMAGTG
                     AELLARPEIRAAYLEGGHA"
     gene            2054..3118
                     /locus_tag="VGC82_03010"
     CDS             2054..3118
                     /locus_tag="VGC82_03010"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY0422271.1"
                     /translation="MGYKIAIMGTGAVGAYAGAHMARAGEDVTFIDPWPANVETMKAK
                     GLTVSHIRDVPEWSTPVRALHLTELQQAAKEKPFDIAFVCMKSYDTEWATVMISQYLQ
                     PAGFIVSLQNCMNEETIAGVVGWGKVVGCIASSITVDLFEPGHVRRAAGKSGDKHTVF
                     RTGEVHGRITDRVQEIKRLVALADSALVTSNLWGERWSKLVTNAMANGMSASTGLISK
                     DILRNDTLRRFGARLGSEAIRVGQALGYELEEVHHIDPEIIARAGEGDADATREYDAH
                     RLDEVSKAGGGEHRPSMGQDMVKGRRTEIDFLNGFIVRKADEIGLRTPANAALTDIVK
                     RVEKGELKPDPKHLIDLRLN"
BASE COUNT          597 a         1036 c         1163 g          601 t
ORIGIN      
        1 gcttcgccgg cgcgtttttc gctacccgcc aggcgttcat cagccccgaa agctttacct
       61 tcatcgagtc cgccaccgtg ctggcgatcg tcgtgctcgg cggcctggga agccagctcg
      121 gcgtcgcgct ggccgccatc gtcatggtcg gcgggctgga ggccttgcgg gactttctga
      181 gtttcctcgg cgaccagatg aacagcccgg tcttcgccgg catcgccagc gaattgccgt
      241 tgtaccggat gttgatcttc ggcctcgccc tggtggtaat gatggtcctg cgcccccgag
      301 gcctggtttc cgggcgcacc cccaccgtgg ccctgacccg catcaaggaa atcgcgccgg
      361 agatcgtggc gcaaggccgc ggataatgct gtcggtgacc aacctgacga tgcgtttcgg
      421 cggccttcgg gccgtggacg atctgtcgtt cgaggctcgg gccggcgata taaccgccgt
      481 cattggcccc aacggtgccg gcaagacgac ggtgttcaac tgcatcactg gcttctaccg
      541 cccgaccggc ggaacggtcg tcctgacgca cccggacggc acgacctatc cgctgcagcg
      601 gatgcaaggt caccgcatcg ccggtttggc caaggtcgcc cggacattcc agaacattcg
      661 gctgttcgcc ggcatgaccg tgctggagaa ccttctcgtc gcccagcaca acaccctgat
      721 ggcggccacc ggttttggcg tactggccgt gctgggcctg ggccggtacc gtgccgccga
      781 ggctgccggc atggaaaagg ctcgcttctg gctggatgcg atcaacttga cggcccgcgc
      841 cgacgatccc gccggcgctc tgccatacgg tgcccagcgc cggctggaaa ttgcccgctc
      901 catgtgcatc gacccggtgc tgctgtgcct ggacgaaccg gcggcggggt tgaacccgcg
      961 cgagtccgag gaactcaaca ggctgttgcg ccgcatccgc gaccagggct gctcgctgct
     1021 gctgatcgag cacgacatgg gcgtagtgat gcggatctcg gaccacatcg tggtgttgga
     1081 tcatggcaag aagatcagcg acggcacgcc cgcggaggtt cgcgccgatc ccgccgtgat
     1141 cgcggcgtat ttgggcgagg gcgaggaaga cgaggtgccg gcatgaccgc gctgctgtcg
     1201 ctgtctggtg taaccgcctt ctacggcgcg atccaggcgc tgcgggggat cgacctggcg
     1261 gtggaacagg gcgagatcgt tacgctgatc ggcgccaacg gggcggggaa atccaccctg
     1321 atgatgacga tctgcggcaa cccccgcgcc cggtcgggga ccatccggca ccagagccag
     1381 gacatcacgc agtggccgac gcatgagatc atgcgccgag gaatcgccca ggcaccggag
     1441 ggccggcgga tttttgggcg tatgacggtg cgcgagaacc ttctgatggg cgcggaggtt
     1501 gccggaaggc gcgatattcc cgaactgctc gatcaggtgt tcaccttgtt tccccgattg
     1561 caggagcgaa tcgcccagcg tggcggcacg ctgtccggcg gcgagcagca gatgctggcc
     1621 atcggacggg cgctgatgtc gcggccgaca ttgctgttgc tggacgagcc gtcgctgggg
     1681 ttggccccgc tggtggtgcg gcaaattttc gcggcgatcc gggagctgaa ccgatcgacc
     1741 ggcttaaccg tgctgttggt ggagcagaat gcgtatcagg cgctgcggct ggcgcaccgc
     1801 gggtatgtgc tggtgaacgg atcgatcacg atggccggga ccggggctga gttgctggcg
     1861 cggccggaga tccgggccgc gtatctggag ggtggccacg cgtagccggg ccggtctggt
     1921 caatcgcgct gctcaagtaa ctacctttgc atcgatacac gcttcgccac tcgtaaagga
     1981 gcggcagcgg ttgcgtttcc cgcgcgggtc ggcagactgt cttcagacgc atcgtcggag
     2041 cggggagaga aacatgggct acaagatcgc gatcatgggc accggtgccg tcggcgctta
     2101 tgctggggcg cacatggcgc gggcagggga ggacgtaacc ttcatcgatc cctggccagc
     2161 aaatgtggag acgatgaagg cgaagggcct gacggtctcc cacatccgcg atgtgccgga
     2221 gtggtccacg ccggttcgtg ccctgcacct gacggagttg caacaggcgg cgaaggaaaa
     2281 gccgttcgac atcgccttcg tctgcatgaa gtcctacgac accgagtggg cgacggtaat
     2341 gatttcgcaa tacttgcagc ccgcgggctt catcgtatcg ctgcagaatt gcatgaacga
     2401 ggaaaccatc gccggcgtcg tcggctgggg caaggtcgtc ggctgcattg ccagctccat
     2461 cacggtcgat ctgtttgaac ccggccacgt gcgccgcgcc gccggcaaaa gcggcgacaa
     2521 gcacacggtg ttccggaccg gcgaagtgca tggccgcatc accgaccgcg tgcaggagat
     2581 caagcgtctg gtcgcattgg cggactcggc gctggtaacg tcgaaccttt ggggcgagcg
     2641 gtggtcgaaa ctggtgacca acgcgatggc caacgggatg tccgccagca ccgggctgat
     2701 cagcaaggac attctccgca acgacaccct gcgtcgcttt ggagccaggt taggtagcga
     2761 ggcgattcgg gtgggccagg cgctggggta tgaactggag gaggttcacc acatcgaccc
     2821 ggaaatcatc gcccgcgccg gcgagggcga tgccgacgca acccgagagt acgatgcgca
     2881 tcgattggac gaggtcagca aggccggcgg cggcgagcac cgcccgtcga tgggccagga
     2941 catggtcaag ggccggcgaa cggagatcga tttcctgaac ggcttcatcg tgcgtaaggc
     3001 ggacgaaatc ggcttgcgga cgccagcgaa cgccgcgctg actgatatcg tcaagcgggt
     3061 tgagaaaggt gagttgaagc ctgatccgaa gcatctgatc gatctgcggc tgaactgacg
     3121 ggcgcggttc cctgggttga ggtaaatcgt ttcatcacgg attgcgcgga ttaaggcacg
     3181 gactcgcacg gagcatcatt tggcggccac gccgacatca ccggcttttg gggcgctggt
     3241 gcgtcgtgaa ctccgtacgc gaggataatt cggccgtgtg gcccccgcct tcgatgaaac
     3301 ctaacgcgcg aagcggcctc ggtcccagaa gcctcggtcc cagaagcctc ggtcccagaa
     3361 gcctcggtcc cagaagcctc ggtcccagaa gccttgg
//
LOCUS       DASWQN010000184         3440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2130868, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000184 DASWQN010000000
VERSION     DASWQN010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2130868"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..744
                     /locus_tag="VGC82_03015"
     CDS             133..744
                     /locus_tag="VGC82_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422272.1"
                     /translation="MAYYMVRLYSGSGNRSPEQLCELAGRELAPQLMQAGGLQRYLTG
                     ITDDGKVVSASVYDSKAAAQKGLDTAREWVSKTDAMRGYQLSQTMQGEIVRVMQGNTQ
                     NRATHGVGRLHTTNATAERIADAIEQHGIPASQSIQGRARTLVVQLEDGRVGTFLAFD
                     SQEGRDQHSQNVRNQRAELNPIKQVMPNEPEEINVRIVTSTSG"
     gene            1682..3109
                     /gene="cls"
                     /locus_tag="VGC82_03020"
     CDS             1682..3109
                     /gene="cls"
                     /locus_tag="VGC82_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEY0422273.1"
                     /translation="MLIAIHILIQAGLITRILLRQHRNPASRIAWIVVVLALPAVGIV
                     AYLLLGETNIGARRAKRLRRILASMPKISQTPGWDAPMLCADIPEFYRPLFNVGRSIS
                     GFEPVGGNEAQLMADSDATIDAMVADIEAACDHVHVLFYIWLPDNNGTRVAEALKRTA
                     NRGVTCRAMVDGLGSRALVRSHLWTEMASSGVKLARALPIDYPLFRVFFGRIDLRNHR
                     KIVVIDNRITYCGSQNCADPAFLPKAKYAPWVDAVMRFEGAVARQNQFVFAADWMTYS
                     DEDIRDLLRQPIEAPTAGFPAQVIASGPTVRYSAMPEMFASLIYSARHELVITTPYYV
                     PDDALQGALCAAGNRGVAVTILFPARNDDFAVAATSRSCYADLLAAGVNIYEYVGGLL
                     HTKSLTLDGQITLIGSANMDRRSFDLNYENNILLSDPALTVEMRRRQDVYIALSKPVT
                     VADVDAWSWWYRLRNNALAMLGPVL"
BASE COUNT          704 a         1037 c         1001 g          698 t
ORIGIN      
        1 ttgagaaccc agggaaggaa attatcgacc tcaacctcgg ccagcaagac cccggcgcta
       61 cataatactt gcatttgtct caaatagcgc gcctatttaa gaagctgcac ttagcagcag
      121 ggaggagaaa tcatggcata ctatatggtg cgcctgtact ccggctcggg aaatcgctct
      181 ccagaacagc tctgcgaact cgccggtaga gaacttgccc cgcaactcat gcaagctggc
      241 ggactgcaac gatatctgac cggaattaca gacgacggca aggtcgtctc ggcatccgtg
      301 tacgacagca aggcggctgc tcagaagggt ttggacaccg ctcgggagtg ggtatcaaag
      361 actgacgcga tgcgtggcta tcaactctcg cagacgatgc agggcgagat tgtacgagtc
      421 atgcaaggca acacgcagaa ccgagcgacc catggggtgg gtcgtctgca caccaccaac
      481 gctaccgcgg agcggattgc tgacgcgatc gagcagcatg gcattccggc ctctcagtcc
      541 atccagggtc gggctcgcac cctcgtggtg caacttgagg acggccgggt cgggacgttt
      601 ctggccttcg attcccaaga aggtcgtgac caacacagcc aaaatgtccg gaaccaacgg
      661 gccgagctga atcccatcaa gcaggtcatg ccgaacgagc cggaagagat caatgttagg
      721 attgtcacat ccacatccgg ctaatctggg aacggtgcgg ggccaccggc ctcaatccga
      781 ctgctggaag ctccgactgg gttgggggtc taggtccggc ggccaaccct tcagattggt
      841 cgaacccact ctaccggtgg gacgcgcaat tcccccgcct agtcccgccg cctcaccacg
      901 gggatctcgc cagtcatcag ctggcttccg gcaggtccgg gacccctttt tgctcgcgca
      961 tgcggcaccg gcggtccaca ggtccggctg gagccggtcc gtgccgatga tgcgcacgtc
     1021 gagccggttg tcgagggttg gctcgaactt cgatcggtag acagggttta tgatgagggc
     1081 gtctgaatga accgcctcgc ctgattgccc gccaaggcgc agctcagctg aaccaaccgt
     1141 ggcacagagt tcctcgacgc tgacgtcaag gagctcggcg gaccgctaca cctccgtgcc
     1201 ctcagcgagg gcgagcgcat cctctatgtc ggcgtccagg tataaacggt gctctcgatc
     1261 ttcgtgtggc tgagcagggt ctggacagcg aaggcattac ggctcctgtt gggcgtcaat
     1321 caacgcctga ggtgcctaga gaacagtaat gaaatgtccc tgaggccccc ttcagggcag
     1381 ccacggaatc ggcggcgcgc caagctgccg agtaagcaga cgctgcacgt ctcggacgat
     1441 tcggcgaggc ggccgcgggt ttcatgcgga tcaatgacca tccagttcga cgactcgacg
     1501 agctaaattt agttcccggc aattgtgtat cctcgccgag gctcaatgcc gatcgcgtgc
     1561 cagacatttg acttcgctta attttcttgg aaccagcggc cgccgattta cgtcaggatt
     1621 tcttgtgtac tatcaaggtg ccgcgattcg caatccgtcg tcacaccacc ggagaccggc
     1681 aatgctgatc gcaattcata tcctcatcca ggccggactc atcacgcgga ttctgctgcg
     1741 ccagcatcgc aatcccgcct cgcggattgc ctggattgtt gtggttttgg ccctgccggc
     1801 cgttggcatc gtcgcgtatc ttctactggg tgagaccaat atcggcgcaa gacgagccaa
     1861 gcgattgcgg cgcatactcg cgtccatgcc aaaaataagc caaacacctg gttgggacgc
     1921 gccgatgctg tgcgcggaca ttcctgaatt ctaccggcca cttttcaacg tgggccgctc
     1981 gatcagcggg ttcgagccgg tcggagggaa cgaggcccaa ttgatggctg attcggatgc
     2041 cactatcgac gccatggtcg ctgacatcga ggccgcctgt gaccacgtcc atgtgttgtt
     2101 ttatatctgg ctgccggaca ataatggcac acgggtagcc gaagccttga agcgcacagc
     2161 aaaccgtggg gtgacctgcc gcgcgatggt cgatggtctc gggtcgcgtg cgcttgtccg
     2221 ctcccatctt tggaccgaaa tggccagcag cggtgtcaag ctggcccgcg cgctgccgat
     2281 cgactatccg ctttttcgag tcttctttgg gcggatcgac ctgcgcaatc accgcaaaat
     2341 tgtggtgatc gacaaccgga tcacctattg cggcagccag aattgtgccg acccagcgtt
     2401 cctgccgaag gcaaaatacg cgccctgggt ggatgccgtt atgcggttcg agggcgccgt
     2461 cgcgcgtcaa aaccaatttg tcttcgcggc cgattggatg acttactccg acgaggacat
     2521 ccgcgatctc ctacgtcaac ccatcgaggc gccgacggca ggttttccag cgcaagtgat
     2581 cgccagcggc ccgaccgtcc gctattcggc catgcctgag atgttcgcga gcctgattta
     2641 tagcgctcgg cacgaattgg tcatcacgac gccctattac gtgccggacg acgcgctgca
     2701 gggagcattg tgtgccgctg gaaaccgcgg ggttgcggtg accatcctgt ttccggcccg
     2761 caatgatgac tttgcagtcg cggccaccag ccgcagctgc tacgcggatc tgctcgccgc
     2821 gggcgtgaac atctacgaat atgttggtgg cctcctgcac acgaagtcgc tgactttaga
     2881 tggccagatt acgctgatcg ggtcggccaa tatggaccgg cgtagcttcg atttgaatta
     2941 cgagaacaac atcctgctct ccgatcccgc gctgaccgtc gagatgaggc gacgccaaga
     3001 cgtttacatt gctctgtcaa agcccgtcac cgtcgccgac gtggacgcct ggagttggtg
     3061 gtacagattg agaaacaacg cactagccat gctagggccg gtgttgtgac atccatcgat
     3121 atataagcca tctacattcg gtttccacct caagcggcca tggtcatacc gctcgaaagg
     3181 ctcgatactt ccaccgggag caggcgaatt gcacataaga aaaatcactt gccaatgctg
     3241 cggacgtgga ttctcagcag tgtcccctgg ttcaggagac accttgcatg gaagcggttc
     3301 tggcgatctt tgccgacatt gtcgatctgc ccgccagcac cacgcccagc cacgacacgt
     3361 tcagccggtt gttccggctg ctcgatcccg aacaactgca agcagcgctc cgccgcttcg
     3421 ccgccgccct gcgcgagggt
//
LOCUS       DASWQN010000185         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1338986, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000185 DASWQN010000000
VERSION     DASWQN010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338986"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VGC82_03025"
     CDS             complement(<1..882)
                     /locus_tag="VGC82_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY0422274.1"
                     /translation="MTVIGVDFGTTNSVVTRLCPDGSVATVRHAFGTTDIDVIRSVLC
                     FWNDGARDRVVLRDAVGPAAIEAYLDDPLECRLMMSLKTYLAQRSFSETRVFGRPFTL
                     ERLIGLFLGAILPKQAFGMSITAGRPVQSAGDAPDDGLGEQRLRASYAAAGWTDVRTA
                     LEPEAAGYRFARGLSAPATVLIGDFGGGTSDFSVLRFEPGSGRPVQALGHAGVGIAGD
                     TFDFRIMDQVVSPLLGKGTTYRPGGTDLPVPPEYYSNFARWHRLSLMRAPKTMRDIEA
                     VARTASHPDRLGALLRLI"
     gene            complement(975..1370)
                     /locus_tag="VGC82_03030"
     CDS             complement(975..1370)
                     /locus_tag="VGC82_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HEY0422275.1"
                     /translation="MTDDAGIITDTLERVAERVGDPMPLVFQRLFRETPELEALFVRD
                     ADGLVRGQMFQVTIESLLDFLGDRSYGANLVQIERVNHIGLGVEPEVFDRFYLTVVAT
                     FKDILGVDWTPEMDAVWARAVGDLTRQIA"
BASE COUNT          284 a          519 c          465 g          238 t
ORIGIN      
        1 gatcagccgc agcaatgccc caaggcgatc cgggtggctc gcggtccgag ccaccgcctc
       61 gatgtcgcgc atggtctttg gcgcccgcat cagcgacagg cggtgccagc gtgcaaagtt
      121 ggagtaatac tccggcggca ccggaagatc cgtgccgccg ggccgatagg tggtgccttt
      181 gcccaaaagc ggcgaaacca cctgatccat gatccgaaaa tcgaacgtgt cgccggcgat
      241 gccgaccccg gcatgaccca gcgcctgaac cggccggccg ctcccgggtt cgaatcgcag
      301 caccgaaaag tcgctggtgc cgccgccgaa atcgccgatt aaaacggtcg cgggcgcgga
      361 caaaccgcgt gcgaaccggt aacccgcggc ctccggctcc agcgcggtcc ggacgtccgt
      421 ccaacccgcc gcggcatagg atgcgcgcag gcgctgctcg cccaatccgt cgtccggcgc
      481 atcgccggcg gattgaaccg gccggccggc ggtgatcgac atgccgaacg cctgcttcgg
      541 caggatcgcg ccgagaaaca gcccgatcaa ccgctccagc gtgaaggggc ggccgaatac
      601 ccgtgtttcg gaaaagctgc gctgcgccag ataggtcttg agcgacatca tcagccggca
      661 ttccagcgga tcgtcgagat aagcctcgat cgccgccggc ccgaccgcat cccgcagcac
      721 cacgcggtca cgcgcgccgt cgttccagaa gcacagcacc gaccggatca cgtcgatgtc
      781 cgtcgtgccg aatgcgtgcc gcacggtggc gaccgaacca tccgggcaca acctggtaac
      841 cacgctgttg gtggtgccga aatcgacccc gatcaccgtc atgtccacga tcccctcctg
      901 acgcgggcgt gacggcgatc gaccgttcgg acctgatcgg tcaggtaatc cgcccggtca
      961 ggcaagctgc ccggtcaggc aatctgccgg gtcaggtcgc ccacggcacg cgcccatacg
     1021 gcgtccatct ccggcgtcca atccacgccc aggatatctt tgaacgtggc caccaccgtc
     1081 aggtaaaacc ggtcgaacac ctccggttcg acgccgaggc cgatatgatt gacgcgctcg
     1141 atctggacca ggttggcgcc gtacgaacgg tcgccgagga aatcgagcag gctttcgatg
     1201 gtcacctgga acatctgccc gcgcacgagg ccatcggcgt cgcgcacgaa cagggcctcc
     1261 agctccggcg tttcccggaa caggcgctgg aacacgagcg gcatgggatc gccaacacgt
     1321 tcggccacac gttccagggt gtcggtgatg atgccggcat cgtcggtcat ggtctctccg
     1381 atgggtttgc catcctgctt gacgacagcc tctatggacc tgccgcggat gcgtggtcca
     1441 ccccaacgtt tcaagatcca cttttgtcgc tggcgggggc agaaccatgc cgcatgaatg
     1501 agtatc
//
LOCUS       DASWQN010000186         1812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_811790, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000186 DASWQN010000000
VERSION     DASWQN010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811790"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VGC82_03035"
     CDS             complement(<1..238)
                     /locus_tag="VGC82_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Paraquat-inducible protein B"
                     /protein_id="HEY0422276.1"
                     /translation="MSEQHPTAVVRSRIERKRRLSLIWAIPVVTVIIGGWLAWNTISQ
                     RGSLITITFETAEGLQANQSHVRHKDVDMGVVQKV"
     gene            complement(250..1632)
                     /locus_tag="VGC82_03040"
     CDS             complement(250..1632)
                     /locus_tag="VGC82_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiA/YebS family transporter subunit"
                     /protein_id="HEY0422277.1"
                     /translation="MGTTSQALAGSIPTAEIPRASTMTASRLYECPDCGQMQILPPLP
                     PGARAVCLRCDAVLRHTRRDPLLLPLALNLSALILFGLGATLTIMSVSTAGQQRAAGL
                     FTGPVELSDYGLWEIAVVVLVTTVAVPLARVLSMLIVLFGLRLARPPSELRTIYAWVR
                     HLRPWSMVEIYLLGLFVAYVRLNAMAAVGLGPAVFALAGLMVIMVLADYTLDDQAVWE
                     AMEPRNRRRRRTLDSNADTPDRPVPNHYRWRIGCDTCGLVSRAAPRMRCTRCGFRLHD
                     RIPASLQRTWAFALAAMVLYIPANIYPVLTVVRLGAGQPSTILGGVRELIEAGMWPLA
                     ALVFFASVAVPVLKLIGLGTLLISTHMGTGWALHDRTILYRIVDTIGRWSMIDIFMES
                     ILVGLVQFGKVASVYPGPGAIAFSAVVILTMLAARSFDPRLMWDSARARSDAGLAQTS
                     VLQTDQAQTG"
     gene            complement(1633..>1812)
                     /locus_tag="VGC82_03045"
     CDS             complement(1633..>1812)
                     /locus_tag="VGC82_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422278.1"
                     /translation="PSPNLQLNQEGICPIHVAIVLRRHCFCFLQSVVVAACFLFATPC
                     YVSAMRSAILLRRVP"
BASE COUNT          375 a          568 c          586 g          283 t
ORIGIN      
        1 cgactttttg cacgaccccc atatcgacat ccttgtgccg gacatgggac tggtttgcct
       61 gcagcccctc ggccgtctcg aaggtgatgg tgatcagcga cccgcgctgc gagatggtgt
      121 tccacgccag ccagccgccg atgatcaccg tcacgaccgg gatggcccat atcagcgaga
      181 ggcgccgctt gcgttcgatt ctggaccgca cgacggcggt agggtgctgc tcgctcatgc
      241 ggtctccgct cagccggttt gggcttggtc ggtttggagc acactggttt gggccaaacc
      301 ggcgtcggat cgggcgcggg cactgtccca catcaggcgc ggatcgaaac tgcgggcggc
      361 gagcatggtc aggatgacca ccgccgagaa cgcgatcgcg cccggcccag gatagacgct
      421 cgcgactttg ccgaattgga ccaggcctac caggatcgac tccatgaaga tgtcgatcat
      481 cgaccagcgg ccgatagtat cgacaatccg gtacaggatt gtgcggtcat gcaatgccca
      541 tccggttccc atatgggttg aaatcagcag ggtgcccaga ccgatcagct tgagcaccgg
      601 aaccgcgacg ctggcgaaga ataccagcgc cgccagaggc cacatgccgg cctcgatcag
      661 ttcgcgcacg ccaccgagga tcgtgctggg ctgaccggcg ccaaggcgga caaccgtcag
      721 caccggatag atgtttgccg ggatgtagag caccatcgcc gccagcgcga aggcccaggt
      781 gcgctggagc gaggcgggta tgcgatcatg cagccggaac ccgcagcgcg tgcaacgcat
      841 gcgcggcgcg gcgcggctga ccagcccgca agtgtcgcag ccaatgcgcc agcgataatg
      901 gtttggcacc ggccggtctg gggtgtcggc attcgaatcc agcgtccggc ggcgccggcg
      961 gttgcgcggc tccattgctt cccacaccgc ctgatcgtca agcgtgtagt cggccagtac
     1021 catgatcacc atcaggccgg ccagggcgaa aaccgctgga cccaagccga ccgccgccat
     1081 cgcgttcagg cgcacatagg cgacgaacag ccccagcagg tagatttcca ccatggacca
     1141 aggacggagg tgcctgaccc atgcgtagat ggtccgcagt tccgaaggcg gccgagccag
     1201 tcgcaacccg aaaagtacga taagcatgga cagcacgcgg gcgaggggca ccgcgacggt
     1261 ggtgaccagc accacgacgg ctatttccca caggccgtaa tcactcaact ccactgggcc
     1321 cgtgaacagt ccggcagcgc gctgttggcc agccgtgctg acggacatga tggtcaacgt
     1381 ggcacccagc ccgaacagga tcaacgcgct gaggttgagc gccaacggca acagcaatgg
     1441 gtcgcgccgc gtgtgccgca gcacggcatc acagcgcaag cataccgccc gtgcgccggg
     1501 aggcaaggga ggcaggattt gcatctgccc acagtccggg cattcgtaaa gcctggacgc
     1561 ggtcatcgtc gaagcccgcg ggatctcggc ggttggaatt gatcccgcaa gcgcctgtga
     1621 tgtggttccc attcaaggga ctcgccggag gaggatcgct gaacgcatcg cactgacgta
     1681 gcacggcgtg gcgaacagga aacaggcggc aaccaccacg ctttgcaaaa agcagaaaca
     1741 atggcgacgt aaaacaatgg cgacgtgaat cgggcagatc ccttcctggt tcagctgcag
     1801 gtttggagac gg
//
LOCUS       DASWQN010000187         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1735096, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000187 DASWQN010000000
VERSION     DASWQN010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735096"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..648)
                     /locus_tag="VGC82_03050"
     CDS             complement(73..648)
                     /locus_tag="VGC82_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEY0422279.1"
                     /translation="MKDTVVVFARAPRLGTVKRRLAQDIGERTALRFHNSTLMGLLRN
                     LAASRRFAVVVAMTPDRVRIRLPAGVRIIPQGRGDLGERMSRTLGRYRRVALMGCDIP
                     EARGADVRAAFRALGTADAVFGPAADGGYWLIALGPRRPADLFGKSRWSSEHALADTL
                     HQFRHHRVGFIRTLSDVDSEADYRRYLARKA"
     gene            complement(645..1298)
                     /locus_tag="VGC82_03055"
     CDS             complement(645..1298)
                     /locus_tag="VGC82_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEY0422280.1"
                     /translation="MTTDLSIIIPTLNAAAHLPATLAAVAGVPEIIVVDGGSIDGTPE
                     RAALAGARVLTAPRGRGNQLATGIAAASHSWLLLLHADTKLAPNWRAAVHAEPSRAGY
                     FRFVLDSPDLRARRLERLVAWRCRVLGLPYGDQGLLIHRDLLARVGGMRALPLMEDVD
                     LVRRLGRDRLVALPADAVTSAVKWEQQGYLRRSARNLGCLSLWFAGVPPRLINRLYG"
     gene            complement(1644..>1867)
                     /locus_tag="VGC82_03060"
     CDS             complement(1644..>1867)
                     /locus_tag="VGC82_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422281.1"
                     /translation="PHLFRRLDWKDFDCVRQIIVCPGVVIDGSHGVHLFFGPLRGRSQ
                     IGQGLVFIDVSSGTPHGIYHALFCLPGAD"
BASE COUNT          402 a          593 c          571 g          301 t
ORIGIN      
        1 ccaatggtcg tggtggacaa ttatggctcc tcccgaatgt aagcaggccc gaatgtaagc
       61 aagatcgtgc gatcaggctt tgcgtgccag gtaacggcgg taatcggctt cgctatcgac
      121 atcggaaagc gtgcggataa atccaacacg gtgatgccga aattgatgca gcgtgtccgc
      181 gagcgcgtgt tcgctcgacc agcgcgactt gccgaacaga tccgccgggc gtcgcgggcc
      241 caacgcgatc agccagtatc ccccatctgc cgcagggccg aagaccgcat cggcggtgcc
      301 caaggcccga aacgccgccc ggacatcggc gccgcgtgcc tcgggaatgt cgcaccccat
      361 cagcgccact ctccgatacc gtcccaaggt tcgggacatg cgttcgccca ggtcgccgcg
      421 accttggggg atgatccgga cgccggccgg aagccggatc cgaacgcggt ccggtgtcat
      481 cgccacgacg acggcaaagc ggcgcgaggc ggccagattg cgcagcaatc ccatcaaggt
      541 ggagttatgg aatcgaagcg cggtccgctc gccgatgtcc tgcgccaggc ggcgctttac
      601 tgtccccagg cgcggtgcgc gagcgaagac cacgaccgtg tccttcatcc gtaaagccga
      661 ttgatcagcc gaggcggcac cccggcgaac cacagcgaca agcatcccag attgcgtgcg
      721 gaacgccgaa gatacccctg ctgttcccat ttcaccgccg aggtaaccgc atccgccggc
      781 aaggcaacca atcgatcgcg tccgagacgc cggacaaggt ccacgtcctc catcaacggc
      841 agcgccctca tgccgcccac gcgggccaac aggtcgcggt ggatcagcag gccctggtcg
      901 ccgtagggca acccaaggac gcggcagcgc cacgcgacga gccgctccaa gcgccgcgcc
      961 cgaaggtccg gactatccag gacgaagcga aaataccccg cccggcttgg ctcggcatga
     1021 accgctgctc gccagttcgg cgctagctta gtgtcggcgt gcaacaacag cagccacgag
     1081 tgcgaggctg cggcgatccc ggtcgcgagc tgattgcctc gaccacgcgg ggctgtcagc
     1141 acccgggcac cggccaaagc ggcccgctcg ggggtcccgt caatgctgcc gccatccacc
     1201 acaatgatct ccggcactcc ggcaaccgcc gccaatgtgg ccggcaggtg cgcggcggcg
     1261 ttcaaagtcg gaatgatgat ggaaaggtcg gtggtcacgg aaaggcgcta actgggtcgt
     1321 aacaaattcg tgcccatttc ccgctccgtg caacgtgcga tgaaacaacg tgcgatgaag
     1381 gaatgcatcg ttgacgtata tcagtccatg acgtgtatca ttgttccaca ggtcgatcga
     1441 gacttcgagt gggatcggac gaaatgcgat cgtaatcatc tgcaacacgg tttgccattc
     1501 gaactggcgg tcatgctgtt cgaccctcga aagacctgat gcaagacttg actaccagga
     1561 agtgcggatg caggcgatcg gaatggtcgg catcgtcaca ctggcgtgcg tttacaccga
     1621 ccgaggctca gtgcggcaga ttatcagtct gcgcccggca aacaaaagag agcgtgataa
     1681 ataccgtgcg gcgttccaga actgacgtcg atgaagacaa ggccctggcc gatctgactt
     1741 cggccccgca atggaccgaa gaacagatgg acgccatggc tgccgtcgat aacgacgcct
     1801 ggacagacga tgatttgtcg aacgcagtca aagtctttcc agtccagacg cctgaacagg
     1861 tgcggac
//
LOCUS       DASWQN010000188         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_679303, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000188 DASWQN010000000
VERSION     DASWQN010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679303"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..238)
                     /locus_tag="VGC82_03065"
     CDS             complement(17..238)
                     /locus_tag="VGC82_03065"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY0422282.1"
                     /translation="MELQESRRSWVNETRSEPAMKSLISKFLKNESGATAIEYGLIAA
                     GIAIAIITAVNGVGTALSTKFDSISTALK"
     gene            380..1270
                     /locus_tag="VGC82_03070"
     CDS             380..1270
                     /locus_tag="VGC82_03070"
                     /inference="COORDINATES: protein motif:HMM:NF001784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY0422283.1"
                     /translation="MTSTPATHRASFAIGDEKLAKRVVDLLTESFFEGQAAIAAFAQP
                     NGLWDITVHFADPPDQASIRDLVGIAAGEDVAGTISFDSVEAKDWVKASLEDLVPVPA
                     GRFVVHGQHDRGRIPPNKLGIEIEAALAFGTGHHGTTRGCLLLLDHVLKAWRPRRVLD
                     LGTGTGVLAIAAARALHEKVLASDIDPVSVEVARENARLNVAGHLVQTITATGFSAPQ
                     FAAHGPFDLVLANILANPLRQLATPMARHLAPSALVILSGLLTPQVPAVIAAYRARGL
                     VPLRHQRIEGWSSLLLRHVK"
     gene            1318..1779
                     /locus_tag="VGC82_03075"
     CDS             1318..1779
                     /locus_tag="VGC82_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422284.1"
                     /translation="MAIEQLAKPVNSWCPHCRPGHGCRIYPERPDECQTFNCMWLVND
                     HLDERWRPNKSKLVLTTSEDGIEVRCDPGFPDAWRKEPFHNEIRQWAVSGETHDVTVV
                     VVIGQKMILVTPDREFDLGVVRPDQRIVRELEGTRVVGATVVSESDLARQA"
     gene            complement(1855..2049)
                     /locus_tag="VGC82_03080"
     CDS             complement(1855..2049)
                     /locus_tag="VGC82_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422285.1"
                     /translation="MPAFTFEKISPPAQASRGPIPPIANKKQRGVIVQMLDRFVEARV
                     KRSLREDKGVIARNPHRPPD"
     gene            2236..>2558
                     /locus_tag="VGC82_03085"
     CDS             2236..>2558
                     /locus_tag="VGC82_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEY0422286.1"
                     /translation="MFEAHFQTFEEPESGVALTARLAAFREELARRKLSGFVIPRADQ
                     QQNEYVAPSEERLAWLTGFTGSAGLAIVLTHEAAVFVDGRYTLQAAKQVDRKAWHIEP
                     LADPPP"
BASE COUNT          465 a          805 c          805 g          483 t
ORIGIN      
        1 taccgaaatg gaacagttat ttcagcgcgg tgctgatgct atcgaacttg gtgctgagcg
       61 cggtgccgac gccgttgacg gcggtgatga tggcgatcgc aataccggcg gcaatcaggc
      121 cgtattcgat ggccgtcgca ccggattcgt tcttgagaaa cttcgaaatg agagatttca
      181 tggctggttc cgacctcgtt tcattgaccc aggaacggcg gctttcctgc agttccatca
      241 aggaaactag cggcagaaat ctaaggttgc tctaacggcg tacgtataag ttttcgggtt
      301 attaagaact gaaatttgct caagttagag ctaaatcacc ccatcggcct ttcgcccgaa
      361 agttccactt ggattttcaa tgacttctac tcccgctacc catcgcgcca gttttgcaat
      421 cggtgacgaa aaattagcca aacgcgtcgt tgacctgctg accgaaagct ttttcgaagg
      481 ccaggccgcc attgccgcct ttgcccagcc caacgggctg tgggacatca cggtccattt
      541 cgccgatccg cccgaccagg cctcgatccg ggatctcgtc ggcatcgccg ccggcgagga
      601 cgtcgccggg acgatctctt tcgactcggt cgaggccaag gactgggtca aggccagcct
      661 cgaggacctc gtccccgtcc ccgccgggcg gttcgtggtg cacggccagc atgaccgcgg
      721 ccgcatccct cccaacaagc tgggcatcga gatcgaggcc gcgctcgcgt tcggcaccgg
      781 ccatcacggc accacgcggg gctgcctgct gctgctcgat cacgtcctga aggcctggcg
      841 cccgcgccgc gtgctcgacc tcggcaccgg taccggcgtg ctggcgatcg ccgcggcccg
      901 ggcgctgcat gaaaaggtgc tggcgagcga catcgatccg gtctcggtcg aggtcgcccg
      961 cgaaaatgcg cggctgaacg tggcggggca tctggtgcag acgattaccg ccaccggatt
     1021 ttcggcgccg caattcgcag cacacggccc gttcgacctg gtgctggcga acatcctcgc
     1081 taatccgctc cggcaactgg cgacgccgat ggcgcggcat ctcgcaccct cggcgctggt
     1141 gatcctgtcg ggcctgctga cgccgcaggt gcccgccgtg atcgccgcct atcgcgcccg
     1201 cggactggtg ccgctgcggc accagcgcat cgaaggctgg agcagcctgc tgctgcgcca
     1261 tgtaaaatga ccgtgcccgg cagggaatgc ggggagtgca cgctgtgctg caaggtcatg
     1321 gcgatcgaac agctcgccaa gccggtgaac tcgtggtgcc cgcattgcag gcccggccat
     1381 ggctgccgga tctacccgga gcggccggac gagtgtcaga cgttcaactg catgtggctg
     1441 gtcaacgatc atctcgacga gcgctggcgg ccgaataaat ccaaactggt cctgacgacg
     1501 tcggaggacg gtatcgaagt ccgttgcgac cctggattcc ccgatgcctg gcgcaaggag
     1561 ccgtttcaca acgaaatccg gcagtgggcg gtgtccggcg aaacccatga cgtgacggtg
     1621 gtcgtcgtta tcggtcagaa gatgatcctg gtgacgccgg atcgggaatt cgatctcggt
     1681 gtcgtccgtc cggaccagcg gatcgtgcgg gagcttgagg ggacgcgtgt cgtcggtgcg
     1741 accgtggtga gcgaatccga tctggcgcgg caagcctgaa cccaagtgcc ctgaacgaaa
     1801 cgggtccggc tatgaaccgg acccgcatct tcgaataagt gtggtggaga tgacctagtc
     1861 gggaggtcgg tgcgggttgc gggcgatcac gcctttgtcc tcgcgcaggc tacgtttgac
     1921 gcgtgcttcg acgaaccggt ccagcatctg cacgatcacc ccgcgttgct tcttgttcgc
     1981 gatcggtgga atcgggccgc ggctggcctg cgccggcggc gaaatcttct caaacgtgaa
     2041 agcaggcatc gtgtatcccc tcttcgttga gttgaaacac tcctggagcc cccctgtacc
     2101 ctagacgaac ggcctgcacc gcgaaccgtt ccgtccgatc tacgcggaca gctggattca
     2161 agcatagatt atgccaaccg gattgatccg gatcagattg cggacccctc cgcccgaccc
     2221 tagagtagcc aagccatgtt cgaagcccat ttccagacat tcgaggagcc cgaaagcggc
     2281 gtcgcgctga ccgcgcggct ggccgccttc cgcgaggagc tggcacgccg aaaactgtcg
     2341 ggattcgtga ttccgcgcgc cgatcagcag cagaatgagt acgtggcgcc gtcggaagag
     2401 cggttggcct ggctgaccgg cttcaccggc tcggcgggtc tggccatcgt cctgacccat
     2461 gaggcggccg tattcgtcga tgggcgctac accctgcagg cggccaagca ggtcgaccgc
     2521 aaggcctggc atatcgagcc gctggccgac ccgccgcc
//
LOCUS       DASWQN010000189         2643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_812008, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000189 DASWQN010000000
VERSION     DASWQN010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812008"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..613)
                     /locus_tag="VGC82_03090"
     CDS             complement(305..613)
                     /locus_tag="VGC82_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422287.1"
                     /translation="MVTHLSIQGEGEKPQFTENKAMTKLKILGAAAMLSLTFATPVFA
                     QEAIRDPGAFAFYHPDDDVLNGGRPAAAAGLDDRTLMQLNSSEAYGTPVNRRHEAFQR
                     "
     gene            complement(1171..1320)
                     /locus_tag="VGC82_03095"
     CDS             complement(1171..1320)
                     /locus_tag="VGC82_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422288.1"
                     /translation="MVEVRHDPERAAHYQRDDQDPKARASTLLVLSGREVEEEHEVHA
                     DLRNR"
     gene            1482..1955
                     /locus_tag="VGC82_03100"
     CDS             1482..1955
                     /locus_tag="VGC82_03100"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEY0422289.1"
                     /translation="MQQHATFTEPTFIRHTDVRPTGKPRGQPFHKLLVSFSAAYFTGA
                     LITDIVYWQTPDPLWERFSIWLICAGLLVAGLGAAAYAIDLAVSRQIDRPAWPRAVGY
                     VVAVLLSLVNAFVHSRDGYTAVVPTGLMLSALVIVVLLLTAGVSMALANRHRIGG"
     gene            1961..>2643
                     /locus_tag="VGC82_03105"
     CDS             1961..>2643
                     /locus_tag="VGC82_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEY0422290.1"
                     /translation="MYELKKAASPKLPRLAALLATVACLGLAGCDDHAGDPKMQIGAN
                     PVLPALQQYLMPPMAVAKVVGWGSETPAVAQGLQVHAVATGFQHPRSLYVLPNGDVLV
                     VESNGPKAPIDRPKDIITGWVQSFAGAKAKDANRITLLRGTKPDGTPELRTVFLDHLN
                     SPFGVALVGHDLYVANTDGIMRFPYQDGQTSITAVGTKLTDLPGGPIDHHWTKALLAS
                     PDGSKLYVGV"
BASE COUNT          513 a          812 c          733 g          585 t
ORIGIN      
        1 actcgaatgg tggaacagaa gtggagactc ggtatcccgg accaactgcg taatcgcacg
       61 agcgaggctg ctatcgggaa cgcggatacc atcgatcgtg agcgtatttt cagtccccaa
      121 cgtcattttg cttctcctgc ctatgttggg acatgcctcc atacatgaag cggcgggttg
      181 gtaaggtcga ttaaataggg atttaattga cgtcggagcg tcgccgctcc agccgctgag
      241 atcgcgcata gaaaaggccg catcattgcc gatgcggctt tttccatcca gcacgctggc
      301 ttcactacct ttgaaatgct tcgtgccgtc tgttcacagg cgtgccatag gcctcgctgc
      361 tattgagttg catcagtgtc ctgtcgtcca ggcccgccgc cgccgccggc cggccgccgt
      421 tcaggacatc atcgtcggga tggtaaaatg cgaacgcacc ggggtcccgg atagcctcct
      481 gcgcgaacac cggcgttgca aacgtcagcg acagcatggc tgccgcacca aggatcttga
      541 gtttggtcat agctttgttc tccgtgaatt gaggtttctc gccttcgccc tgaatactca
      601 ggtgcgtcac cacggaggta agtcgaagct ctgccgatcc cactaaataa cgctttagga
      661 aactaacttg aatttatgtc gacggcttga gatgagcacg cgccacgtct gtgaggacgc
      721 ttttggttgg ctgtcttaac ttgcaattta gtggcgccca cagacgggtg ccctagatca
      781 cctcaaggta acgctctcac gatttattgg agtttcgtaa tgtccagagc ctcggcaaaa
      841 atcattcttg cggtcctcct tgtcctttta cccgccgctt cattcgcgag gacggccggc
      901 gaagcgggca tgggtaacgt gcccgtcagc ggaattccac cgggtcctgc taacgtcggt
      961 ggcatgaaca atgcgaccgt cgatccgagt ggaatcgcaa atgcttctaa gttcgctccg
     1021 ctgcccccgc cgcgcatatc tgtcccgcca attccacagt tcaagtgaga cgacggctgc
     1081 tgattttcac ggcgccgtgc cggcggattc tttcttcgta gccggccctg atatgtgtgg
     1141 ccgccgagca ttccggtagc acctgtcgtg ctagcgattg cgcaaatcgg catgcacctc
     1201 gtgttcttcc tccacctcac ggccggacaa caccaatagc gtgctggccc tggccttcgg
     1261 gtcctgatcg tcacgctggt aatgggcggc tcgctctgga tcatggcgaa cctcaaccac
     1321 aacatgatgc cgatcgatct gctcatgcag cgctagaaat tctgatcatc tcggaagtta
     1381 aaaccgaatt tagaagaccg gcgcacaaac ccgaagcaat cttgcaagga ccacaccacg
     1441 tttccatggc gcccattagt ccgtctggag gatcgagaga gatgcaacag cacgccacgt
     1501 tcacggaacc gacgttcata cgccataccg atgtgagacc aactgggaag cctcgtggac
     1561 aaccgttcca taaactgctg gtgtcgtttt ccgctgctta tttcaccggc gctctcatca
     1621 ccgatatcgt ctattggcag acccccgatc cgctgtggga gcggttctcg atctggctga
     1681 tctgcgccgg actgcttgta gccggccttg gcgccgccgc ctatgcgatc gaccttgcgg
     1741 tcagcagaca aatcgacagg ccggcgtggc ctcgcgcggt tggttacgtg gtcgccgtcc
     1801 tgctctctct ggtcaatgcc ttcgttcaca gccgcgacgg ttacaccgcc gtggtgccga
     1861 cggggctgat gctttccgcg ctcgtcattg tcgttctctt gttgacagcc ggggtttcca
     1921 tggccctggc aaatcgccac cgtattggag gataatcgtg atgtacgaac tcaagaaggc
     1981 agcttcgccg aagctgccgc gtctcgccgc gctccttgcc accgtcgcct gcctcggact
     2041 cgccggctgt gacgaccatg ccggcgaccc gaaaatgcag atcggcgcca atcccgtgct
     2101 gcctgcgctg cagcagtatt tgatgccgcc gatggcggtc gcaaaagtcg tcggatgggg
     2161 gagcgaaact cctgcggtag cgcagggatt gcaggtccat gcagtggcta ccggctttca
     2221 gcatccccgg tccctctacg tcctccccaa tggagacgtg ctggtggttg agagcaacgg
     2281 cccgaaagcg ccaatcgacc ggcccaagga catcatcacc ggctgggtac agtctttcgc
     2341 cggcgccaag gccaaggacg caaaccgcat cacgttgctg cgcggtacca aacctgacgg
     2401 cactcccgaa ttgaggaccg tgtttctgga ccacctcaac tcgccatttg gcgtcgccct
     2461 ggttggccat gatctctacg tggccaatac tgacggcatc atgcgctttc cgtatcagga
     2521 cggacagacc agcatcacgg ctgtgggcac caagctcacc gatcttcccg gtgggcccat
     2581 cgatcatcac tggaccaagg ctctgctggc cagtccggac ggctccaagc tctacgtcgg
     2641 ggt
//
LOCUS       DASWQN010000190         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_547834, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000190 DASWQN010000000
VERSION     DASWQN010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547834"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VGC82_03110"
     CDS             complement(<1..264)
                     /locus_tag="VGC82_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEY0422291.1"
                     /translation="MTAGHKRSAHPKRSATPSKDFQPEQSPQAATECQAAPIEDYALI
                     GDCRTAALVSKQGSIDWLCWPRFDSPACFAALLGSPDNGRWKIA"
     gene            complement(264..629)
                     /locus_tag="VGC82_03115"
     CDS             complement(264..629)
                     /locus_tag="VGC82_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422292.1"
                     /translation="MQIGHRIKAARISVAIACAAFLGMAGGSAEARVFVGIGIPFYGP
                     GYYAPPPVYYPPPPIYYAPPPVVYAPPRPYTSAPPGVGAGQVCYAGPYSCPMDNPVAT
                     GGSCYCLGNGGQRVWGRAN"
     gene            905..>2252
                     /locus_tag="VGC82_03120"
     CDS             905..>2252
                     /locus_tag="VGC82_03120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEY0422293.1"
                     /translation="MFADTMLSDPAQVLRQVFGFPGFRGQQDAAVRHMVAGGDALVLM
                     PTGGGKSICYQVPALVRPGTAVIVSPLIALMDDQVAALRQVGVNAGALHSEIDPAEAR
                     SITRDLIEERLDLLYVSPERLLGNGTLDRLSRVRLALFAVDEAHCVSQWGHEFRPEFR
                     ELARIPDMFPGVPRIALTATADPRTQQDILTALRMDRAEVFVSSFHRPNLRLSAAAKI
                     GETAQLFEFLKSHHGECGIVYCGSRAKTERVAAKLSEKGFPALPFHAGLDPLHKRESL
                     TRFRSGEPLVVVATIAFGMGIDRPDVRFVVHLDMPDSPEAYYQQIGRAGRDGDPADTL
                     LLYGGQDVAQARHWLVQSAAPEARKRVMRTKLEEMVALTEAVTCRTRSLLTCFGEALP
                     CDCGHCDNCEAPPVTVDASTEAQMVLSAVYRTDQIFGAAHIVSVLRGERTEGVLRHG"
BASE COUNT          378 a          671 c          778 g          425 t
ORIGIN      
        1 cgcgatcttc cagcgcccgt tgtccggact tccaagcagg gcggcgaaac aggcgggact
       61 gtcgaagcgg ggccagcaaa gccagtcgat cgagccttgc ttgctgacga gagccgcggt
      121 gcggcaatcg ccgatcaggg catagtcctc gatcggggct gcctgacatt ctgtagcggc
      181 ttgcggactt tgttccggtt ggaaatcttt agaaggggtt gccgagcgtt tggggtgggc
      241 ggaccgcttg tggcccgccg tcatcagtta gcccgccccc acacgcgctg cccgccattg
      301 ccaaggcaat agcagcttcc gccggtcgca accggattgt ccatcggaca ggaatagggt
      361 ccggcgtaac acacctggcc tgcccccacc ccaggcggtg ccgaggtgta gggccgcggc
      421 ggtgcataca cgaccggcgg cggggcgtag tagatcggcg gtggcggata atataccggt
      481 ggcggcgcgt agtagcccgg cccgtagaat ggaatgccga tcccgacgaa gacccgcgcc
      541 tcggcggaac cgccggccat acccagaaaa gccgcgcatg ctatcgcgac tgaaatccga
      601 gcggctttga tccgatggcc gatttgcatg gacataacgt tcccgactgt gccggttgcc
      661 tgtatcttca ttatgtccga tttcgacccg actggatacc atgattacgc aaggcgacct
      721 cgccggttgc atggcctcgg cgcacggttg cgatagacga tcggcaggcc gtatcccgat
      781 tgctgccggt gggcgctgag atggtgaaca tccacacagc gttggcgaca ggtgggtgca
      841 ggcgcctata ctgcttgagc tacccggcga ttcggtcgcg tggtacagga gtgtccaagt
      901 cgcgatgttc gccgacacca tgcttagcga tccggcgcag gtgttgcgtc aggtttttgg
      961 cttcccaggc tttcgcggtc aacaggacgc ggcggtgcgg catatggtcg cgggcggcga
     1021 tgcgctggtg ctgatgccga ccggtggcgg taaaagcata tgctaccaag tgccggcctt
     1081 ggtccggccg ggcacggcgg tgatcgtctc gccgctgatc gcgctgatgg acgatcaggt
     1141 cgcggccttg cgtcaggtcg gggtcaatgc tggcgcgctg cactcggaaa tcgatccggc
     1201 cgaggcacgc tcgatcaccc gtgacctgat cgaagagcgg ttggacctac tgtacgtttc
     1261 gcccgaacgg ttgttgggca acggcacgct ggatcggctg tcgcgggttc ggctggcgct
     1321 gttcgcggtg gacgaggcac attgtgtttc ccagtggggt catgaattcc gcccggaatt
     1381 ccgcgaactg gcccgcatcc ccgatatgtt tcccggcgtg ccgcgcatcg cgctgacggc
     1441 gaccgccgat ccgcggacgc agcaggacat cctgacggcg ctacgaatgg atcgggcaga
     1501 ggtgttcgtt tccagcttcc accggccgaa tttgcggctt tcggcggccg cgaagatcgg
     1561 cgagacggcg cagttgttcg agttcctgaa aagccaccat ggcgaatgcg ggatcgttta
     1621 ttgcggcagc cgggcgaaaa ccgaacgggt ggcggcgaaa ctaagcgaaa aaggctttcc
     1681 ggcgttgccg ttccacgccg gcctcgatcc gttgcacaag cgggagtcgc tgacccggtt
     1741 ccgctccggc gagccgctgg ttgtggtggc gacgatcgcg ttcggaatgg ggatcgaccg
     1801 cccggatgtg cgcttcgtgg ttcacctgga catgccggac agcccggagg cgtattacca
     1861 gcagatcggc cgggcggggc gggatggcga tccggcggac acgctgctgc tatatggtgg
     1921 tcaggatgtt gctcaggcca ggcattggct cgtgcaatcg gctgcacccg aagcccgcaa
     1981 gagggtcatg cggacgaagc tggaggaaat ggtcgcgctg accgaggcgg tgacatgccg
     2041 cacccgctcg ctgctgacct gcttcggcga ggcgctgccc tgcgattgcg gccactgcga
     2101 caattgcgag gcaccgccgg tcaccgtcga tgcctcgacc gaggcgcaga tggtgctgtc
     2161 ggcggtgtat cgcaccgatc agattttcgg cgctgcccat atcgtatcgg tgctgcgcgg
     2221 cgagcggacc gagggcgttt tgcggcatgg cc
//
LOCUS       DASWQN010000191         4437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2131429, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000191 DASWQN010000000
VERSION     DASWQN010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2131429"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1482)
                     /gene="ccmI"
                     /locus_tag="VGC82_03125"
     CDS             complement(118..1482)
                     /gene="ccmI"
                     /locus_tag="VGC82_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEY0422294.1"
                     /translation="MILPFLLALIAFAALLPIVVPLLRGSRPVASRGSFDQAVYRDQL
                     RELDRDTSRGLITSTEASAARLEIQRRLLAADRLPAAPSRLSRSPILAAFVFTGVAAG
                     SVSCYLWFGSPGLPDEPFSGRNAEVARDDGQAALQQATDTLGAKLKQNPTDSSGWLLY
                     GRSLAMLNQWDQAEDAYRHAIDLGETGPDVAVAHAEIMVMQAGGTVTPAAESAFRQVL
                     TASPNNGLARYYLALAAMQAGEPRKAIDGFQALLAELPADSPLRSQLARKVAEAAQAA
                     GTPVPELAKGAQAAVPGPDANAVADAANMTDDQRQAMVRGMVANLAAKQEADPTNLEG
                     WLRLARAYVVLHEPEKAAAAFDKAARLRPDDVSIPLQEVRALLSDRPPTEKLPPRVID
                     LLKRVEAADADQPLVLWYLGMAAAQDARADEAQRYWTRLLTKVPEGGDDAKMIQSAID
                     AIKR"
     gene            complement(1479..1943)
                     /locus_tag="VGC82_03130"
     CDS             complement(1479..1943)
                     /locus_tag="VGC82_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEY0422295.1"
                     /translation="MRAWFLALLLLFAALAVQAVEPRERLADPALEARARTISGELRC
                     LVCQNESIDESGADLAHDIRVFVRERLSAGDSDARAMQAIVSRYGSFVLLKPPVEPAT
                     FVLWYGPPLLVAIAGAGAVLWLRRRRSTPAEAAPLSPEESRRVEGLMRGNDA"
     gene            complement(1940..2476)
                     /locus_tag="VGC82_03135"
     CDS             complement(1940..2476)
                     /locus_tag="VGC82_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEY0422296.1"
                     /translation="MRRTFFLLPLGIFLVIVTYFSVSLRPGHDIHELPSAMIEKPAPA
                     FDLVGLADSRPLALAALKGHPFVINFFASWCIPCRIEHPLLMRLAEQNHLPLYGVAYK
                     DKPEDASRLLATFGDPYRQIGVDQDGRVGLDFGVYGVPETYVIDSTGQIRRRFVGPLT
                     AGTVNKELLPLMKQLDPS"
     gene            complement(2479..>4437)
                     /locus_tag="VGC82_03140"
     CDS             complement(2479..>4437)
                     /locus_tag="VGC82_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY0422297.1"
                     /translation="ILALFVAIIQSTVPLAGAARRHLGWMAVGRSCALTGFALTALAM
                     LALMHAYVTSDFTVINVIENSHTDKPLLYRITGVWGNHEGSMLLWVFMLSLCSAAVAL
                     FSDNLPPALRARVLAVQGMIAVGFLLFILVTSNPFLRTFPPAPNGHGLNPVLQDPGLA
                     FHPPFLYLGYVGFSVAFAFAVAALIEGRVDAAWARWVRPWTLASWCALTVGIAMGSWW
                     AYYTLGWGGWWFWDPVENASFMPWLVGTALIHSSIVVEKRDTLKSWTVLLAIITFSLS
                     LVGTFLVRSGVLTSVHAFATDPERGTFILLLLIAAIGGSLTLYAIRAPALKGGGLFAP
                     LSREGALVLNNLLLSCGCATVFLGTLYPLFLDAMNGPKLSVGFPFFNRTFAPMMVPMV
                     IAVGVGPMLAWKRGDLLGALQRLWVAYIATALVVLVMFYLTYGGPVMAVLGLGLAAWL
                     STAVLTEFAERIRLFRIPLADSLRRAINLPRATYGMTIAHFGLAVSVAGFAASAFDKE
                     AIEILKPGGSIAIAGYELTLEHVDRLQGPNYTADDASIRVTKDGAVVTTMHPERRFFP
                     LRKQTTGETAIRTNLLADLYVALGESDTKGDWTVRVYWKPLVPWIWIGAIIMAFGGVV
                     SLSDRRWRVGVANKASRRPSQAVPAAGE"
BASE COUNT          856 a         1430 c         1479 g          672 t
ORIGIN      
        1 tcaggccgcg ctcgattcct gcctggccag ttagcgcttc ctgtaacgga cgcccggcac
       61 gatatcctgg ctcgcgatcg ttatgatgcg atcgttatag ggggcgggcg ggtctgatca
      121 acgcttgatc gcatcgatgg ccgactggat cattttcgcg tcgtcgccgc cctcgggcac
      181 ttttgtcaac agcctggtcc agtagcgttg ggcttcatca gcgcgggcat cctgtgccgc
      241 ggccatcccc agataccaca gcaccagcgg ctggtcagcg tcggcggcct cgacacgctt
      301 cagcaaatca atcacccgcg gtggcagttt ttccgtcggt ggccggtcgc tcagcagcgc
      361 gcgaacctcc tgcaggggaa tcgagacgtc gtctggtcgc agacgtgccg ccttgtcaaa
      421 agctgcggcg gctttctcgg gctcatgcag aacgacatag gcgcgggcga ggcgcagcca
      481 gccctccagg ttggtcggat cggcttcctg cttggccgcc agattggcga ccattccgcg
      541 gaccatcgcc tgccgctggt catcggtcat gttcgccgcg tccgccacag cattggcgtc
      601 cggtcccggt acggccgcct gtgctccttt cgccagttca ggcaccggag tgccggcggc
      661 ctgcgcggcc tccgcaacct tccgcgcaag ctggctgcgc agcggagaat ccgccggtag
      721 ttcggccagc agtgcctgaa acccgtcgat cgccttgcgt ggctcgcccg cctgcatggc
      781 ggccaaggca aggtaatagc gcgcgagccc gttgttggga ctggcggtca gcacctgcct
      841 gaacgcgctc tctgccgcgg gtgtgacggt gccgcccgcc tgcatgacca taatctcggc
      901 atgggcgacg gccacgtccg gaccggtctc gcccaggtcg atcgcatggc gataggcgtc
      961 ttccgcctga tcccattggt tcagcatcgc caggctgcgg ccatagagca gccagccgga
     1021 cgaatcggtc gggttctgct ttagcttggc tcccagggtg tccgttgcct gctgcaatgc
     1081 agcctgaccg tcgtcccgtg cgacctctgc gttgcgcccc gaaaacggct cgtcaggcag
     1141 tccgggcgag ccgaaccaca aataacagga aaccgaccct gcggcgacac cggtgaagac
     1201 gaaagccgcc aggatcgggc tgcgggacaa cctggacggc gccgcgggca gcctgtccgc
     1261 ggcgagcagg cggcgctgga tctccaacct tgcggcactg gcttccgtgg acgtgatcag
     1321 gccgcgggaa gtgtcgcggt ccagttcgcg caactggtcg cggtagacag cctgatcgaa
     1381 gctgccccga gaggcgaccg gccggctgcc gcgcagcagc gggacgacga tcggcagcaa
     1441 tgcggcaaag gcgatcagcg ccagaaggaa gggcaagatc aagcgtcgtt tcctcgcatc
     1501 agtccctcga cgcgacggct ttcctcggga ctaagcggcg cggcctccgc cggcgtagat
     1561 cggcgacgcc tcagccacag caccgcaccg gcaccggcga tggccaccaa cagcggcggg
     1621 ccataccaaa gcacaaaggt cgccggctcg accggcggct tcagcagcac aaacgatccg
     1681 tagcggctga cgatggcctg catcgcccga gcatcggagt cgccggcgga aagcctctcg
     1741 cgcacgaaca cgcggatgtc gtgcgcgagg tcggcgccgg attcgtcgat cgactcgttc
     1801 tggcaaacca ggcagcgcag ctcgccgctg atcgtcctgg cgcgggcttc caaggctgga
     1861 tcggccagcc gttcgcgggg ctccacggcc tggaccgcca acgcggcgaa aagcagcaac
     1921 agggcgagga accaagccct catgatgggt cgagctgctt catcaaaggc agcaattcct
     1981 tgttcacggt gccggcggtc agggggccga cgaagcggcg gcggatctgg ccggtgctgt
     2041 cgatgacgta ggtctccggc acgccataga cgccgaaatc cagcccgacg cgaccgtcct
     2101 ggtccacgcc gatctggcga tacggatcgc cgaaagttgc tagcaggcgc gacgcgtcct
     2161 cgggcttgtc cttgtacgcg accccgtaca gcggcagatg gttctgctcc gccagccgca
     2221 tcagcagcgg gtgctcgatc cggcagggga tgcaccagga ggcgaagaaa ttgatgacga
     2281 aggggtggcc cttcagcgct gccaacgcca gtggccgcga gtcagccaga ccaacaaggt
     2341 cgaaggccgg tgccggcttt tcgatcatcg ccgatggaag ttcgtggata tcgtgccccg
     2401 gccgcagcga aacggaaaaa taggtcacga tgaccaggaa aatccccagc ggcaggagga
     2461 agaaggttcg ccgcatgact attcgccggc cgccggcacc gcctgagacg gtcggcgaga
     2521 cgctttgttg gcaacgccaa cgcgccagcg acggtcgctc aggctgacca ccccgccgaa
     2581 cgccatgatg atggcgccga tccagatcca gggaacgagc ggcttccaat agacgcggac
     2641 ggtccagtcg cccttggtgt cggattcgcc cagggccacg tacaggtcgg ccagcagatt
     2701 ggtgcggatc gcagtctcac cggtggtctg cttccgcagc gggaagaacc ggcgttccgg
     2761 atgcatcgtg gtcaccaccg cgccgtcctt tgtgacccgg atggaggcat cgtcggcggt
     2821 atagttgggg ccctggaggc gatccacatg ctccagcgtc agttcatagc cggcgatggc
     2881 gatgctgccg ccgggtttca gaatttcgat ggcttccttg tcgaacgcgg aggcggcgaa
     2941 accggcgacg gacaccgcga ggccgaaatg ggcgattgtc atgccgtagg tggcgcgcgg
     3001 caggttgatg gcccgtcgca agctgtccgc cagcggtatc cggaacaggc ggatgcgttc
     3061 ggcgaactcc gtcagcacgg cggtggacag ccacgcggca aggcccaggc ccagcaccgc
     3121 catcactggg ccgccatagg tcaggtagaa catcaccagc acgaccagcg cggtggcgat
     3181 gtaggcgacc cagagccgct gcaatgcgcc cagcaggtcg ccgcgtttcc aggccagcat
     3241 ggggccgaca ccaacagcga tgaccatcgg caccatcatc ggtgcaaaag tgcggttgaa
     3301 gaacgggaag ccgaccgaca gtttcgggcc gttcatggcg tccaggaaca aggggtagag
     3361 cgtgcccagg aatacggtcg cgcagccgca ggacagcagc aggttgttca gcaccagcgc
     3421 gccctcccgc gacaaaggcg cgaacagccc gccacctttc aaggcaggcg cacggatcgc
     3481 atacagcgtg agcgatccac cgatggcggc gatcagcagc agcaggataa aggtgccacg
     3541 ttccggatca gtagcgaaag catggaccga ggtcaggacg ccggaacgga ccaggaacgt
     3601 gccgaccagc gaaagtgaga aggtgatgat cgccagcagg accgtccagg atttcagcgt
     3661 gtcgcgcttc tcgacgacga tggacgaatg gatcagcgcg gtgccgacca gccaaggcat
     3721 gaacgacgcg ttttccaccg ggtcccagaa ccaccagccg ccccatccta gcgtgtagta
     3781 ggcccaccac gaccccatcg cgatcccgac ggtcagcgca caccacgacg ccagcgtcca
     3841 gggccgtacc cagcgggccc aggcggcgtc gacccggcct tcgatcaggg cggcgacggc
     3901 gaaggcgaaa gcgaccgaga aaccgacata gccgaggtag aggaatggcg gatgaaacgc
     3961 gaggcccgga tcctgcagga ccgggttcag cccgtggccg ttcggcgctg gcggaaaggt
     4021 gcggagaaac ggattggatg tgaccaggat gaacagcagg aaaccgaccg cgatcatgcc
     4081 ctggaccgcc agtacccgag cgcgaagggc agggggcaga ttgtcgctga acaatgcgac
     4141 cgcggccgaa cagagcgaga gcatgaagac ccagaggagc atggagccct cgtggttacc
     4201 ccagacgccg gtgatccggt acagcaacgg cttgtcggtg tggctgttct cgatgacgtt
     4261 gatgaccgtg aagtccgacg ttacgtaggc gtgcatcagc gccagcatcg ccagcgcggt
     4321 cagcgcgaag ccggtcagcg cgcaggatcg ccccacggcc atccagccga gatgccgccg
     4381 agcggcaccg gccagcggga cggtcgattg gatgatggcg acaaaaagcg ccaggat
//
LOCUS       DASWQN010000192         3582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1999536, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000192 DASWQN010000000
VERSION     DASWQN010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999536"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..762
                     /locus_tag="VGC82_03145"
     CDS             94..762
                     /locus_tag="VGC82_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0422298.1"
                     /translation="MHYQWDFVFLLRYAPLFGRGVLVTLGYTAATIFIGLIIGLAVGL
                     GRLSRSRLLNIPLVAYIEAFRCTPLLVQIVWFYYALPVLLGIQIPAVVAGILTLSCYT
                     GAFYAEIFRGGIISIEQGQWDAARALGLRRWHVMRRVILPQAIRRMIPPFVNQSITQL
                     KNTSLISTIAVPDLLYNGTLVTADTYRPLEVYTVVALIYFALLFPSTLLAQAYERRLA
                     SRPR"
     gene            771..2447
                     /locus_tag="VGC82_03150"
     CDS             771..2447
                     /locus_tag="VGC82_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422299.1"
                     /translation="MPSSLLETIEGRRLADRDVWLRWGPYLAERQWGTVREDYSATGD
                     AWTYFPHEHARSRVYRWGEDGIAGWTDDRLRWCLSLALWNGRDPILKERMFGLTNAQG
                     NHGEDVKELYYYLDGTPTHSYMRMLYKYPQTAFPYADLIAENARRGLDQPEYELLDTG
                     VFQDNRYFDVTVEYGKAAPEDILIRITIDNRGPDAADLHVLPQLWARNTWDWRPGIPE
                     PDLMLSDGGVIASHNRMADRRLDIDTAASWLFCRNETNTKRLYGDDAAGPFKDGINDY
                     VVDGETSAIGRQRGTKCAAHVKLKMAPGARHVLRLRWRPTAADDPFADHDELFAARLT
                     EADAFYAALQTNIADPDARLVQRQALAGMLWSKQFYRYDVRRWLGGDPLQPPPPSERL
                     QARNADWQHLNNRDIISMPDTWEYPWYASWDLAFQAVTFALIDPEFAKQQLLLLTHEW
                     FMHPNGSLPAYEWAFGDVNPPVHAWAAWRVFRMDAALRGAPDFEFLERIFHKLLINFT
                     GWVNRQDVEGRNVFQGGFLGLDNIGVFDRSQKLPVAGRIDQSDGTSWMAM"
     gene            2451..3437
                     /locus_tag="VGC82_03155"
     CDS             2451..3437
                     /locus_tag="VGC82_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422300.1"
                     /translation="MNMMRISLELATRNHVYESTATKFFEHFLAIAEAMTRQNGRQGL
                     WDDVDKFYHNVLQRPDGSAIPLRLFSLVGLFPLFAVEVLEPETVARCPEFAQRLRWVL
                     DHRPDLAALVSHWDIAGQGSRRLLSLLRGSRMKALLRRMLDEKAFLSPYGVRSVSRIH
                     LDAPFELDLDGQHFSVGYMPGDSNSRAYGGNSNWRGPVWMPVNFMLIEALYGFHRYYG
                     DDFRVEYPTGSGVTLSLSQIAQQLSLRLTRLFLRQPDGRRPIFGNNALLRDDPHFRDN
                     ILFYEFFNGDTGEGLGASHQTGWTGLVALLLHGREADDPTLMAMPTGEGAPV"
BASE COUNT          645 a         1094 c         1119 g          724 t
ORIGIN      
        1 acaacgtcag ccgcgaccag gtgccgccag gcttaacgtt ctaaccgtcc caggccccgg
       61 tcctgccagc gcgcgtcgag agtcggcctt ctgatgcatt atcaatggga cttcgtcttc
      121 ctgctccgtt acgcgccgct gttcgggcgc ggcgtgctgg taacgctggg ctacacggcg
      181 gcgacgatct ttatcggctt gatcatcggc ctggcggttg gactgggccg gttgtcgcgc
      241 tcacggctgc tgaatattcc tctcgtggcc tacatcgaag cgttccgctg caccccgttg
      301 ctggtgcaga tcgtctggtt ctactacgcg ctgccggtgc tcctgggcat ccagattccg
      361 gccgtggtcg ccggcatcct gacgttgtcc tgctacaccg gggcgttcta cgccgagatc
      421 ttccgcggcg gcatcatctc catcgagcag ggccagtggg atgccgcccg agcacttggt
      481 ttgcggcgct ggcatgtgat gcggcgggtg atcctgccgc aggcgatccg gcgcatgatc
      541 ccgcccttcg tcaaccaatc gatcacccag ttgaagaaca cgtcgctgat ttcgacgatc
      601 gcggtgccgg acctgcttta caacggcacc ctggtgacgg cggacaccta ccggccgctg
      661 gaggtttata cggtggtcgc cttgatctac ttcgcgttgc tgtttcccag cacgctgctg
      721 gcccaagcct atgaacggcg gctggcgtcc cgtcctcgat aggaagtcac atgccgagca
      781 gcctgctgga aaccattgaa ggaaggcgtc tggcggaccg ggatgtctgg ctgagatggg
      841 ggccctacct cgcggaacgg caatggggaa cggtgcggga ggattattct gcgaccggcg
      901 acgcctggac ctatttcccg catgagcacg cccgcagccg ggtctatcgc tggggcgagg
      961 acggtatcgc cggttggacc gacgaccggc tgcggtggtg cctgtcgctg gcactatgga
     1021 acggacgcga tccaatcctg aaggagcgga tgttcggcct gaccaacgcc cagggcaacc
     1081 atggcgagga cgtcaaggaa ctctactact acctcgatgg cacgccgacc cattcgtaca
     1141 tgcgaatgct gtacaagtac ccgcagacgg cgtttcccta cgccgacctg atcgcggaga
     1201 atgcccgcag ggggctggat cagccggaat acgaactact ggacaccggt gttttccagg
     1261 acaaccgtta tttcgacgtg acggtcgagt acggcaaggc ggcgccggag gatatcctga
     1321 tccggatcac catcgacaac cgtggtccgg acgcggcgga cctgcatgtg ctgccgcaac
     1381 tctgggcacg caacacctgg gactggcggc cgggtattcc ggaacccgat ttgatgctat
     1441 ccgatggtgg cgtcatcgcg tcgcacaacc gcatggcgga ccgccgcctg gacatcgaca
     1501 ccgccgcaag ctggctgttc tgccgtaacg aaaccaacac gaaacgcctt tacggcgacg
     1561 atgccgcggg tccgttcaag gacggcatca acgactatgt cgttgatggt gaaaccagcg
     1621 cgatcggccg gcagcgcggc accaaatgcg ccgctcatgt gaaactcaaa atggcgccgg
     1681 gtgcgcgcca tgtgcttcgg ttgcgttggc gtcccacagc ggcggatgat ccgttcgccg
     1741 atcatgacga gctgttcgcg gcgcgcctga ctgaggccga tgctttctac gccgcactgc
     1801 aaaccaatat cgctgatccc gacgccaggt tggtgcagcg ccaggcgttg gccgggatgc
     1861 tgtggtccaa gcagttctac cgatacgacg tgcgccgctg gctgggcggc gatccgctgc
     1921 aaccaccgcc gccttcggaa cggctgcaag ctcggaacgc cgactggcag cacctcaaca
     1981 accgcgatat catatccatg ccagatacct gggaatatcc gtggtatgcg tcctgggacc
     2041 tcgcgtttca ggcggtgacg ttcgcgttga tcgacccgga gttcgccaag cagcagcttt
     2101 tgctgctgac gcatgaatgg ttcatgcacc cgaacgggtc gctgccggcc tacgagtggg
     2161 cgttcggcga cgtgaacccg ccggtgcatg cctgggcggc ttggcgcgtc ttccgtatgg
     2221 atgcggcgct gcgaggcgcg ccggatttcg agttcctgga acgcatcttt cataagctgc
     2281 tgatcaactt caccggctgg gtgaaccgcc aggacgtgga aggccgcaat gtcttccagg
     2341 gtggcttcct cgggttggac aatatcggcg tgttcgaccg cagccagaag ctgcccgtcg
     2401 ccggccgcat cgaccagtcg gacggtacgt cctggatggc gatgtaagcc ttgaacatga
     2461 tgcgcatctc gttggagctt gccacccgca accatgtcta tgaatcgacg gcaaccaagt
     2521 tcttcgagca tttcctggcg atcgccgaag ccatgacaag gcagaacgga cgccagggat
     2581 tatgggacga tgtcgataaa ttctaccaca acgtgctcca gcgccctgac gggtcggcga
     2641 tcccgctgcg tttgttctcg ttggtcggac ttttcccgct gttcgccgtg gaggtactgg
     2701 agcccgaaac ggtggcgcgt tgcccggaat ttgctcaacg gctgcgttgg gtactggatc
     2761 accggccgga cctcgcggca ctggtctcgc actgggatat agcaggacaa gggtcgcggc
     2821 gtttgctgtc cctgctgcgg ggcagccgca tgaaggcgct gctgcgacgg atgctggatg
     2881 aaaaagcgtt tctttcgccc tatggggtgc gaagcgtgtc tcgaatacat ttggatgcgc
     2941 ccttcgaact cgatctggac ggccagcatt tcagcgttgg ctacatgccg ggcgacagca
     3001 acagccgggc gtatggtggg aactcgaact ggcgcgggcc ggtctggatg ccggtgaatt
     3061 tcatgctgat cgaagccctg tacgggtttc atcgttacta tggcgacgac ttccgggtgg
     3121 agtatccgac cggatcgggc gtcaccctta gtctgagcca gatcgcgcaa caactgtcct
     3181 tgcgcctgac tcggctgttc ctgcgccaac cggacggtcg ccgtccgatc ttcggcaaca
     3241 acgcgttgct gcgcgacgat ccgcatttcc gcgacaacat cctgttctat gaattcttca
     3301 atggcgacac cggagaaggg ctgggcgctt cgcaccagac cggctggacc ggccttgtgg
     3361 ccctactgct gcacgggcgg gaggcggacg atcccacgct catggcaatg ccaactggag
     3421 aaggcgcgcc cgtctgatcc gcacctgctt tccgtggttg gaaagtagta cagtgttact
     3481 gatattcggg gatcagtgga atgaccctcg ggaagctcac ttatcccctc atttcaaacc
     3541 acctcgaaaa gtccgctgaa cgatccgcac tttgagacgg tc
//
LOCUS       DASWQN010000193         6772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2131917, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000193 DASWQN010000000
VERSION     DASWQN010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2131917"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /gene="mutL"
                     /locus_tag="VGC82_03160"
     CDS             complement(<1..1161)
                     /gene="mutL"
                     /locus_tag="VGC82_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY0422301.1"
                     /translation="MSRIRLLSESTVNRIAAGEVIERPAAAVKELVENALDAGATRIA
                     VCLDGGGIDRLEVTDNGIGMSADELALCVLRHATSKLADETLVRITTLGFRGEALPSI
                     GAAARLRITSRPNGRPAGAAPGTADDADHANVISVEGGHVSAVAPTAGAAGTRVVVRD
                     LFFATPARRKFLKSPRIEAEHAEAVVRRLALSAPAVAFRLELDGRVVFEAPAQERIER
                     VAALVGAEAAAVMLPVQEEREGVRISGYICSPSVTRATPVGQTLIVNARPVADPVLRV
                     AVKVAYRDVIAAGRHAIVALYLDVPPDELDVNVHPAKTELRFRDPAGVRSLVIGSVRR
                     ALGVGTGQVVPMPGPGPGWTAAFASRSGRPSHAAPPMAMSLSPPPGALPQGAF"
     gene            1604..2119
                     /locus_tag="VGC82_03165"
     CDS             1604..2119
                     /locus_tag="VGC82_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEY0422302.1"
                     /translation="MDDDQTQRPDSLLPYEKWIEHALRHVVAQAIEHAAVHGLPGGHH
                     FYITFRTAYPGVELPPRVRAQYPKDMTIVLQHQFWDLAFNRETQAIQVGLSFGGVPTK
                     LVIPLDAVMEFADPHIRYGLRFQAPEGPEANDIRPASPPAGGEPEAPTKDGEPAQVVS
                     LDAFRRRSPQD"
     gene            2168..3769
                     /locus_tag="VGC82_03170"
     CDS             2168..3769
                     /locus_tag="VGC82_03170"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEY0422303.1"
                     /translation="MFPLAADATPWRKLPIEGLRTLTVEGRTVLRIAPEALSELALQA
                     FHDVSHLLRPAHLASLRRILDDPEASPNDRFVALDLLKNANIAAGGVLPMCQDTGTAM
                     VFGKKGQRVWVDGDEEEALSWGVHRTYTETNLRYSQMAALSMFEEVNTGNNLPVQFDI
                     MAAPGEHHADEFSLMFVAKGGGSANKTFLFQETRAILSPERLAKFLETKMKTLGTSAC
                     PPYHLAIVIGGTSAELTLKTVKLASTKWLDGLPTKGSRSGHAFRDLELEQQILRISRD
                     TGIGAQFGGKYFCHDVRVVRLPRHGASLPVGIGVSCSADRQILAKITPDGVFLEQLET
                     DPAKYLPEATDIQLGDDAVAIDLDRPMAEIRAELSRHTVKTRLSLTGTMVVARDIAHA
                     KLKERLDNGLTLPDYLKDHPVYYAGPAKTPTGMATGSFGPTTAGRMDSYVAAFQEAGG
                     SLVMLAKGNRSRAVKDACKKHGGFYLGSVGGPAARLAQDCIRKVEVLEFPELGMEAVW
                     RIEVENFPAFIVMDDKGNDFYDGLG"
     gene            3769..4170
                     /locus_tag="VGC82_03175"
     CDS             3769..4170
                     /locus_tag="VGC82_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0422304.1"
                     /translation="MRFTIDRIDHIVINCRSVEISAAWYQRVLGMEREDFGPHNRTAL
                     KFGGQKINLRPIESDVGTWPTGVCAEAGAADLCFITAVPPDEVIGHLHDNGVQILQGP
                     VDRAGALGPIRSVYCRDPDGNLIEVSSYQSL"
     gene            4188..5318
                     /gene="queA"
                     /locus_tag="VGC82_03180"
     CDS             4188..5318
                     /gene="queA"
                     /locus_tag="VGC82_03180"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY0422305.1"
                     /translation="MPENPRASLTLADFDYHLPPECIAHTPARPRDSARMLHVRDDGF
                     GDLSIADLPALLRPGDVLVANDTRVFPAQLQARRGAARIGITLDRPRADGAWKALARN
                     GRRLQVGDSLSILASAVRLASAAPSAPRSAETLGPLTARVVDKDPDGSIGLAFNLDGD
                     ALMQAFQQAGALALPPYIHRPDGPLPSDATDYQTVFAARPGAVAAPTAGLHFTSALLA
                     RLEAAGIRRVTVTLHVGIGTFLPVRVEQVTDHRMHPERGEITEEAAAAINRGRRAGGR
                     VVAVGTTSLRLLESAALPDGTVRPFLGETDLFILPGYRFRTADRLVTNFHLPRSTLLM
                     LVSAFRGREQILAAYAHAVAAGYRFHSYGDACFLDRADAQSF"
     gene            complement(5911..6531)
                     /locus_tag="VGC82_03185"
     CDS             complement(5911..6531)
                     /locus_tag="VGC82_03185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0422306.1"
                     /translation="MMETSFWLIRHALVEENARAFLYGVMDVPLCEPTLLEQAPMYRS
                     LAARLPRPADWLVTPLSRTRRTAETIFAHGYPDTELGVEPDLIEQSLGEWQGLPHHEL
                     PPRLLHPAHAFWPLAGQEKPPGGESMAEVIARVGAAMERLAKTHRGRDVVIVSHGGSI
                     RGAIAHALRIGPDNALHLSIQNLSLTRLQKTPDGWRVQCVNELPGY"
     gene            complement(6528..>6772)
                     /locus_tag="VGC82_03190"
     CDS             complement(6528..>6772)
                     /locus_tag="VGC82_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0422307.1"
                     /translation="SAVFYPVSVLPAFLRPIAYALPTTHVFEGMRAVLSLGIVRWDHL
                     AAAFALNMVWLAAAALLFTRQFHAARVSGALISIGE"
BASE COUNT         1178 a         2345 c         2170 g         1079 t
ORIGIN      
        1 gaaggcgccc tgtggtaagg cgccgggtgg tggcgacagc gacatcgcca tcggcggcgc
       61 ggcatgggac ggccggccgg atcgcgacgc gaacgcagcg gtccaccccg gccccggtcc
      121 cggcatcggc accacctgcc cggttcccac ccctaatgcc cgccgcaccg agccgatgac
      181 cagcgagcgc acaccggcgg gatcgcgaaa ccgcagctcg gtttttgccg gatgcacgtt
      241 cacgtcgagt tcgtcgggcg gcacatccag atagagcgcc acgatggcat gccgccctgc
      301 cgcgatcacg tcgcggtagg cgaccttgac cgcgacccgc agcaccggat cggccaccgg
      361 ccgggcgttg acgatcagcg tttgccccac cggcgttgcc cgggtgaccg atggcgagca
      421 gatatagccg ctgatccgga cgccctcgcg ttcctcctgc accggcagca tcaccgcggc
      481 ggcttccgcc cccaccaggg cggccacccg ctcgatccgc tcctgtgccg gcgcttcgaa
      541 caccacgcgt ccgtccagtt ccagccggaa cgccaccgcg ggcgccgaca gcgccaggcg
      601 gcgtaccacc gcctcggcgt gttccgcctc gatccgtggg cttttgagga atttccgccg
      661 cgccggggtg gcgaaaaaca gatcccgcac caccacgcgg gtgcccgccg ctcccgcagt
      721 cggggcgacg gcggatacat ggccgccttc caccgaaatc acgttggcat ggtctgcgtc
      781 atctgcggtc ccgggcgcag caccggctgg acggccattt ggacgggaag tgatccgcag
      841 ccgcgctgcc gcgccgatcg acggcagggc ctcgccgcgg aagcccagcg tggtgatccg
      901 caccagcgtc tcatcggcaa gctttgaggt ggcgtgacgc aggacgcaga gcgccagttc
      961 gtcggccgac atgccgatgc cgttatcggt gacctcgagc cggtcgatgc cgccgccgtc
     1021 gaggcaaacc gcgatgcgtg tggctccggc gtcgagcgcg ttttccacca gttccttgac
     1081 cgccgcggcc ggccgctcga tgacctcgcc ggcggcgatg cggttgacgg tgctctcgga
     1141 caggaggcgg atacgcgaca tgcggctgct caggcgtccc cgtgcggttt cggcccgcac
     1201 atccttaggc gtccccgtgc ggtttcggcc cgcacattct acggcgacgt ccgatcaagc
     1261 cctgcgtgaa cgagtcgtgt ccattgcctg gccaagcgcg gcattcgccg gcagccggcg
     1321 cggcggttcg agaacgacgg gataccgggt cggccatggc gttcattgta gcgctttacg
     1381 cctgttgccg aaatgggacg atgcggaata gcacgaagcg cccgatcggc cccggtgcct
     1441 cgcagcacca ctcaccgcgc gtcaacgcga cgataaagcc gagtcgaaaa cgccatcctg
     1501 gataaatcca gtcaaatcaa caaccgcccg cctgacgcgg caagcccctt tgcccgcggc
     1561 ccggtacgcg ctatgggtgg cgccgacagt cgcgagggca ctgatggacg acgatcagac
     1621 gcagcggccg gacagtctgc tgccctatga gaaatggatc gagcatgcgt tgcgccatgt
     1681 cgtggcacag gccatcgagc atgccgcggt gcatggcctg cccggcgggc accatttcta
     1741 catcacgttc cgcaccgcct accctggcgt ggagctgccg ccccgggttc gcgcgcaata
     1801 ccccaaggac atgaccatcg tcctgcagca ccagttctgg gacctggctt tcaaccgcga
     1861 aacccaggcg atccaggttg gcctgtcgtt cggcggcgtg ccgaccaagc tggtcatccc
     1921 attggacgcg gtgatggagt tcgccgaccc gcatatccgc tacggcctgc gcttccaggc
     1981 gccggaaggg ccggaggcga acgacatccg gccggcatcg ccacccgctg gcggcgaacc
     2041 ggaagcgccc accaaggacg gcgaacccgc tcaggtggtc agtctggatg ctttccgccg
     2101 ccgctccccg caagattgaa ggccgtccgt ggacactcag cgccccgccg gtttttcgta
     2161 ttcaccgatg tttccattgg cggcggacgc cacgccctgg cgcaaactgc cgatcgaggg
     2221 cctgcgcacc ctcaccgtcg agggcaggac cgtgctgcgg atcgcccccg aagcgctcag
     2281 cgaactggcg ctgcaagcct tccatgacgt gtcgcatctg ctgcgcccgg cgcatctggc
     2341 gtcgttgcgg cgcatcctgg acgatccgga agcatccccg aacgaccgct tcgtcgcact
     2401 ggatctgttg aaaaacgcca atatcgccgc cggcggcgtg ctgccgatgt gccaggacac
     2461 cggcaccgca atggtgttcg gcaagaaggg ccagcgcgtc tgggtcgatg gcgacgagga
     2521 agaggcattg tcctggggcg tgcaccgcac ctacaccgag accaatctgc gctattcgca
     2581 gatggcggcg ctttcgatgt tcgaggaagt caacaccggc aacaatctgc cggtgcagtt
     2641 tgacatcatg gccgcgcccg gcgagcacca tgccgacgag ttttccctga tgttcgtcgc
     2701 caaggggggc gggtcggcca acaagacatt cctgttccag gaaacccgcg ccatcctgtc
     2761 gccggagcgg ctggcgaaat tcctcgaaac caagatgaag acgctgggga cctcggcctg
     2821 cccaccgtat catctggcca tcgtcatcgg cgggacgtcg gccgaactga cgttgaagac
     2881 ggtgaagctg gcatcgacga agtggctcga cgggttaccg accaagggca gccgatccgg
     2941 ccatgccttc cgcgatctcg aactggaaca gcagatcctg cggatctccc gcgatactgg
     3001 gatcggcgcc cagttcggtg ggaaatactt ctgtcatgac gtgcgggtgg tgcggctgcc
     3061 gcgccatggc gcctcgttgc ccgtcgggat cggcgtctcc tgcagcgcgg accggcagat
     3121 cctggccaag atcacgcccg acggcgtgtt cctggaacag ctcgaaaccg accccgcgaa
     3181 gtacctcccg gaggcgacgg acatccagct aggggacgac gccgtggcca tcgacctcga
     3241 ccggccgatg gcagagatcc gggcggagct aagccgccac accgtgaaga cccgcctgtc
     3301 gctgaccggc accatggtgg ttgcgcgcga catcgcgcat gcgaaactga aggaacggct
     3361 ggataacggc ctgacgctgc ccgactacct gaaggaccac ccggtctatt acgccggccc
     3421 ggcgaaaacc ccgacgggaa tggccaccgg cagcttcggc ccgaccaccg ccggccgcat
     3481 ggacagctac gtcgcggcgt tccaggaagc cggcggatcg ctggtgatgt tggcgaaggg
     3541 caaccggtcc agggcggtga aggatgcctg caagaagcac ggtggcttct acctcggcag
     3601 cgtcggaggc ccggccgcgc ggctggcgca ggactgcatc cgcaaggtcg aagtgctgga
     3661 gttccccgaa ctcggcatgg aggcggtctg gcggatcgag gtcgagaatt ttcccgcgtt
     3721 catcgtcatg gacgacaagg gcaacgattt ttacgatgga ttgggatgat gcgcttcacc
     3781 atcgacagga tcgaccacat cgtcatcaac tgccgaagcg tggagatcag cgccgcctgg
     3841 taccagcgcg tgctgggcat ggagcgcgag gatttcggcc cgcacaaccg caccgcgctg
     3901 aagttcggcg gacagaagat caacctgcgg ccgatcgagt ccgatgtcgg tacctggccc
     3961 accggcgtgt gcgccgaagc cggcgcggcc gatctgtgtt tcatcaccgc ggtgccgccc
     4021 gatgaggtga tcggacacct gcacgacaac ggcgtgcaga tcctgcaagg gccggtggat
     4081 cgggccggcg cgctcgggcc gatccgctcg gtgtactgcc gcgatcccga cggcaacctg
     4141 atcgaggtct cgtcctacca gtcgctgtaa cgccatgcgg atcttccgtg cctgaaaacc
     4201 cccgtgcttc ccttacgcta gcggatttcg actaccacct gccgcctgaa tgcattgccc
     4261 acacccccgc caggccgcgc gactccgcac gcatgctgca cgttcgcgac gatggcttcg
     4321 gtgatctctc gatcgccgac ctgcctgccc tgttgcgtcc cggcgacgtt ctcgtcgcca
     4381 acgacacgcg ggtgtttccc gcgcaattgc aggcgcggcg cggcgcggcc aggatcggta
     4441 tcaccctcga ccggccccga gcggatggcg cctggaaggc tctcgcccgc aatggccgcc
     4501 ggcttcaggt cggcgacagc ctgtctatcc tggcttcggc agtgcgcctg gcttcggcgg
     4561 cgccatcggc gccacgatcg gcggagacac tggggccgct gactgctcgg gtggtggaca
     4621 aggatccgga cggcagcatc ggcctggcgt tcaacctcga cggcgacgcc ttgatgcagg
     4681 cattccagca agccggagcc ctggcactgc ctccgtatat tcaccgtccg gatggtccgc
     4741 tgccaagtga cgcaaccgac taccagacgg tgttcgcggc gcgtcccggc gcggtggcgg
     4801 cgccgaccgc gggactgcat tttacttcgg ccttactggc gaggctggag gcagccggga
     4861 tacggcgcgt caccgtcacg ttgcatgttg ggatcggcac gttcctgccg gtacgggtgg
     4921 agcaggtgac cgaccaccgg atgcacccgg aacgtggcga gatcaccgaa gaagccgcgg
     4981 cggcgatcaa ccggggccgg cgcgccggtg gccgagtcgt ggcagtggga acgaccagcc
     5041 tcaggctgct ggaaagcgcc gcgctgccgg atgggaccgt gcgacccttc ctgggcgaga
     5101 cggacctgtt catcctgccc ggctaccggt tccgaacagc ggaccggctg gtaacgaact
     5161 tccatctgcc ccggtccacc ttgctgatgc tggtctcggc gttccgcggg cgcgagcaaa
     5221 tcctggcggc ctacgcacac gcggtcgccg ccggataccg gttccattcg tacggcgacg
     5281 cgtgcttcct cgaccgcgcg gacgctcagt cgttttgatg ccccccgccc gtggcgccgg
     5341 agacatgcgc cgcattgact gaatttgcgg atcaggactc gtgcaccgcc gatggcacaa
     5401 tcactggcgc cataataagg tgtcacgtct cataccaggt cgataatccg gctcttaggc
     5461 gattccggct gcgcttcggt tcgctatcaa ggtccgaacg gcccctcgca ccggaagctt
     5521 gggcgcgatc cggaccaatg ccgtccggtc cctatccacc gttcgctgga acccgagcgg
     5581 actgacgacg catagatcac cgagaagttt gcctcgcgcc tcgggatgtc gcaccggcgg
     5641 atcccccaca agccgcgcag cggccccaac gcaaggtccc gatcgtcaac agcgaggcag
     5701 gagacttcag caaggcagga cttccttctc cacgccctcc gtgcactctg tgttgaagcc
     5761 ttcgccagcc gccgcacctg tgtcgaaggc cgctcccccg acaaccaccc cggccgcaca
     5821 acctccagca tcgaaaccgc ggcgcaagat ccgcctcgac ctcaaagccc gccggtcatc
     5881 ctttccccat gcgacgccgc ccgcgccgga tcaatacccc ggcagctcgt tgacgcactg
     5941 cacccgccac ccgtccggcg tcttctgcaa acgggtcagt gacaggttct ggatcgacag
     6001 atgcagcgca ttgtcggggc caatccgcag cgcatgcgcg atggctccgc ggatcgagcc
     6061 gccatggctg acgatgacca catcgcgacc gcgatgggtt ttggccagcc gctccatcgc
     6121 cgcaccgacg cgcgcgatga cctctgccat gctctcgcct ccgggcggct tctcctggcc
     6181 ggcgagcggc cagaaggcat gcgcggggtg cagcagcctg ggcggcagtt cgtgatgcgg
     6241 aagaccttgc cattcgccaa gcgattgctc gatcaggtcg ggctcgacac ccagttccgt
     6301 gtccggatag ccgtgggcga agatcgtttc ggcggttcgc cgcgtgcgcg acagcggtgt
     6361 gaccagccag tcggccggac ggggcaacct ggcggccaac gagcgataca tcggcgcctg
     6421 ctccagcaag gtcggctcgc aaagcggcac gtccatcacg ccgtacagga aggcacgagc
     6481 attttcctcc accagcgcat ggcggatcag ccagaacgag gtctccatca ttcaccaatg
     6541 cttatcaggg cgccgctgac ccgcgcggcg tggaactgcc gcgtgaacag caaggcggca
     6601 gcggccaacc ataccatgtt cagcgcgaac gcggcagcca gatggtccca ccggacgatc
     6661 cccagcgaca gcacagcccg catgccctcg aacacgtggg tcgtcggcaa cgcataggca
     6721 atcggccgca gaaatgccgg cagcaccgaa accggataga atactgcaga ga
//
LOCUS       DASWQN010000194         3382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1471911, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000194 DASWQN010000000
VERSION     DASWQN010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471911"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VGC82_03195"
     CDS             complement(<1..296)
                     /locus_tag="VGC82_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422308.1"
                     /translation="MPCLRIHTLAITRVVAVFGMICVTAVSPKSARAADPQPYTVTLK
                     PSGDGALDAALQGSSTLISLRESAPVAGFALAERARQDTQRFKTALHSFGYYKAA"
     gene            426..1376
                     /locus_tag="VGC82_03200"
     CDS             426..1376
                     /locus_tag="VGC82_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0422309.1"
                     /translation="MSTRSRLFPGSNRTPGYLLQLPAGREGWLVARRARAVRRAVAIV
                     AWTLPAMLIQALCLILPGHAKIGFARLYWAIFTRLMGIKVRVIGTAVRRPGSRPVVYV
                     SNHSSWVDVPVVGGVLQGCFVAKGDVATWPVISMIARLGRTVFVSRSRASTGKEREAM
                     RLVLKAGDNLILFPEGTSSDGSRVLPFRTSFFALAEAGGGEDPMALPMIQPVSVVYDR
                     LGGLPAGRASRPVFAWYGDMDIASHFWRLSQHTGLRATVLLHAPLDPARFADRKALSQ
                     AVWQIVADGASTLRQNRPASPLDLPRLRPPAIPAVEPALV"
     gene            complement(1393..2658)
                     /gene="tyrS"
                     /locus_tag="VGC82_03205"
     CDS             complement(1393..2658)
                     /gene="tyrS"
                     /locus_tag="VGC82_03205"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY0422310.1"
                     /translation="MTTNNFLDDTASFLDEATARGFVFQCTDTEALRAAMRAGAITAY
                     IGFDCTADSLHVGSLVQIMILRLLQKHGHKPVVLLGGGTTRIGDPSGKDEARQMLTDE
                     TIAANMVGIRRCFTPFLRFGDGPSDAMLANNADWLDALGYIPLLRDVGIHFTINRMLG
                     FDSIRLRLDREQPLTFLEFNYMILQSYDFRELSRRHGVTLQIGGSDQWGNIVAGMELT
                     RRTDARAVYGLTTPLITTASGGKMGKTAQGAIWLTRDRLSAYDYWQFWRNTEDGDVGR
                     FLRLFTDLPLPEIARLEALGGAEINEAKKVLATEATSLAHGAEQAALAAETARRAFEE
                     GEAAATLPSVTVAAAELEAGIPAFRLFVIAGLAASNAEARRLIRGGGARINDVAVTEE
                     GQPIAADRLQDGAIKLSAGRKQHRLVKPG"
     gene            2717..>3382
                     /locus_tag="VGC82_03210"
     CDS             2717..>3382
                     /locus_tag="VGC82_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEY0422311.1"
                     /translation="MPRTIGLMSGTSLDGVDAAWLDTDGIDIHATGPAITVPYDDALR
                     SELRTILDLAPTLSPDDPRLLAATERLTEYHVRAVEALGCEADLIGFHGQTILHQPHR
                     RRTWQIGDAAMLARRLGIPVVHDFRSADVEAGGQGAPLAPVYHNALAANLPKPLAVLN
                     IGGVANVTWIGSNGVLLAFDTGPGNGPLDDWVFRHTGQAFDEGGMLANSGRVDQAVLA
                     RLLS"
BASE COUNT          569 a         1039 c         1121 g          653 t
ORIGIN      
        1 gcagccttgt agtagccgaa gctatgaagc gcggttttga agcgctgggt gtcctgccgc
       61 gcccgttccg ccaatgcgaa accggctacg ggtgccgatt cccgaagcga gatcagcgtc
      121 gaactacctt gcagcgcagc atccagcgca ccgtcgccgc ttggtttcag cgtcaccgta
      181 tagggttgcg gatccgctgc ccgcgccgac ttgggcgaaa ctgctgtgac gcagatcatt
      241 ccaaagaccg cgaccaccct cgtgatggca agcgtgtgaa tgcggaggca gggcatgcgg
      301 aaccttacac aaaagatagt atcaaatttc cctgaacggt cacggttacg acgttgtgcc
      361 gccgtatccg tgggactatc cgcgccgcca tggaaaatga atcgaatggg ggccggaaaa
      421 ggcaaatgag cacacgctcc aggctgtttc cgggttccaa tcgcactccg ggatacttgc
      481 tgcagttgcc ggctggccgg gaaggctggc tggtggcgcg cagggcgcgg gcggtacggc
      541 gcgctgtcgc catagtggca tggacgcttc cggcaatgtt gatccaggcc ttgtgcctga
      601 ttttgcccgg acacgcgaag atcggttttg cccggctgta ttgggcgata ttcacccgac
      661 tgatgggcat caaggtccgc gtcatcggga cagccgtccg gaggccgggg agccggccag
      721 tggtttatgt atccaatcac tcgtcctggg tcgatgttcc cgtcgtcggc ggcgtgcttc
      781 agggctgttt cgtcgccaag ggcgatgttg cgacgtggcc ggtcatcagc atgatcgctc
      841 gcctggggcg gacggttttc gtgtcacgat cgcgtgcctc caccggcaag gagcgcgagg
      901 cgatgcgcct ggtgctgaag gcaggcgaca acctgatctt gtttcctgag gggaccagtt
      961 cggatggctc gcgagtactg ccgtttcgga cctcgttctt tgcgcttgcc gaggccggtg
     1021 gcggcgaaga cccaatggca ttgccaatga tccagccggt gtcggtggtg tacgaccgct
     1081 tgggcggact gccagcggga cgagccagtc ggccggtatt cgcctggtat ggcgatatgg
     1141 acatcgcctc gcatttttgg cgcttgtcgc agcataccgg gcttcgcgcg accgtgctgt
     1201 tgcatgcgcc gctcgatccc gcgcggttcg cggaccgaaa ggcgctatct caggcggtgt
     1261 ggcagatcgt ggctgacggc gcatccacat tgcggcagaa ccgtccggcg agtcctttgg
     1321 atctgccgcg gcttcgtccg cccgcgatac cggccgtcga accggctctc gtttgaggcc
     1381 atcgcctgcc gattatcccg gcttgaccag acgatgttgc tttcggccgg ccgaaagttt
     1441 gattgccccg tcctgcaggc gatcggcggc gatcggctgc ccctcctcgg taaccgcgac
     1501 gtcgttgatc cttgcaccgc caccacggat caagcggcgt gcctcggcat tgctggcggc
     1561 caaccccgcg atgacgaaca gccgaaacgc cggaatgcca gcctcgagct ccgccgccgc
     1621 cacggtcacg ctgggtagag tcgcggccgc ctcgccctcc tcgaacgccc gtcgggcggt
     1681 ttccgcggcg agtgccgcct gttcggcgcc gtgtgcgagg gacgtcgcct cggtcgcgag
     1741 gactttcttc gcttcgttga tctcggcgcc accgagcgct tccaggcggg cgatttccgg
     1801 caggggcaga tcggtgaaca ggcggaggaa gcggccgacg tcgccgtctt cagtgttgcg
     1861 ccagaactgc caataatcgt aggcggacag acggtcgcgg gtcagccaga tcgcgccctg
     1921 ggcggtcttg cccatcttgc cgccggacgc ggtggtgatc agcggggtcg tcaggccata
     1981 gaccgccctg gcgtcggtcc gccgggtcag ctccattccc gcgacaatgt tgccccattg
     2041 atccgatccg ccgatctgca gcgtgacccc atggcggcgg ctgagctcac ggaaatcata
     2101 gctttgcagg atcatgtagt tgaattcgag gaacgtcagc ggttgctcgc gatccagccg
     2161 cagcctgatc gaatcgaaac cgagcatgcg gttgatggta aaatggatcc ccacgtctcg
     2221 cagcagcggg atgtagccca gtgcgtccag ccagtcggcg ttgttcgcca gcatcgcgtc
     2281 cgacggtcca tcgccgaacc gcaggaacgg ggtgaagcaa cgccggattc cgaccatgtt
     2341 cgcggcgatg gtttcgtcag tcaacatttg gcgggcctcg tccttgccgg acggatcgcc
     2401 aatgcgggtc gttccgccgc ccagtaggac cacgggcttg tggccatgct tctgcaaaag
     2461 ccggaggatc atgatctgca cgaggctgcc gacatgcagg ctgtccgcgg tgcagtcgaa
     2521 accgatataa gcggtgatcg cgccggcccg catggcagca cgcagagcct cggtatcggt
     2581 gcactggaag acgaagccgc gggcagtggc ttcgtcgagg aaggacgcgg tatcgtcgag
     2641 aaaattattg gtcgtcatgg cggttgaccc gtagcttgcg gccgaagcgg gttatcacac
     2701 agcggggcga gggcaaatgc cgcgaacgat cggattgatg agcggcacat cgttggatgg
     2761 cgtcgatgcc gcttggctcg ataccgacgg catcgatatc cacgcgaccg gtccggcgat
     2821 cacggtgccc tacgacgatg cgctgcgcag cgaactgcgg acgatcctcg acctcgcgcc
     2881 aacgctttcg ccggacgatc cgcgtcttct ggccgcgacg gaacggctta ccgagtatca
     2941 tgtgcgggct gtcgaagcgc ttgggtgcga agccgatctg atcggttttc acgggcaaac
     3001 catcctgcat caaccgcatc ggcggcgaac ctggcagatc ggcgacgccg ctatgctggc
     3061 gcgcaggctg ggcattcccg ttgtgcatga tttccgttcg gcggatgtcg aggccggagg
     3121 gcagggcgcg ccgctggcac cggtttacca taatgccttg gccgccaacc ttcccaagcc
     3181 gctggcggtg ctgaatatcg gcggcgtggc gaacgtgacc tggatcggca gcaacggcgt
     3241 tctgctggca ttcgacaccg gcccgggaaa tggtccactc gacgactggg tattccgcca
     3301 taccggtcag gcgttcgacg aaggcgggat gctggccaac tccggccgcg tcgatcaggc
     3361 tgtcctggcg cggttgcttt cc
//
LOCUS       DASWQN010000195         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_943947, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000195 DASWQN010000000
VERSION     DASWQN010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943947"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VGC82_03215"
     CDS             complement(<1..374)
                     /locus_tag="VGC82_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY0422312.1"
                     /translation="MVIVFGSINLDLIFSLPNIPRPGETVLGPSTRIEPGGKGANQAV
                     AASRDGADVVLAGAVGRDALADEALVLLRSARVNLDRVCRVEASTGCAAIAVDPAGEN
                     AIAVGSGANLFVRASQIEDALLA"
     gene            complement(527..1408)
                     /locus_tag="VGC82_03220"
     CDS             complement(527..1408)
                     /locus_tag="VGC82_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0422313.1"
                     /translation="MLPESGPTSRIYFSQRLRLHYVDWGNAGAPPLILLHGGRDHCRN
                     WDWVASDLRRDYHVIAPDLRGHGDSAWSASGHYTMANFIYDLAQLVHQQNLAPVSIIA
                     HSLGGNIALRYTGIYPENVRRIVAIEGLGPGPGQDAVADTKPIADRMRAWIDAQRSNS
                     GRQPRRYPTVEAAYARMQEENKHLTPEQARHLTQYGVNQNEDGTYSWKFDHYVRLWPP
                     YDMTRAAIAELWGAITCKTLLVYGTESWAKNPAEDGRLDYFSNASVLAVEKAGHWVHH
                     DQLDFFLRQVRAFLSQP"
BASE COUNT          351 a          668 c          616 g          355 t
ORIGIN      
        1 gccagcagcg catcctcgat ctgggaggcc cgcacgaaca ggttggcgcc ggagccaacg
       61 gcgatggcgt tctcgcccgc cggatccacg gcgatcgcgg cgcatccggt gcttgcctcc
      121 acccggcaga cccgatccag atttacccgc gcgctccgca gcagcaccaa tgcctcgtcg
      181 gccagcgcgt cgcgaccgac cgcaccggcc aaaacgacat ccgcgccgtc gcgagaagcg
      241 gccaccgcct ggttggcccc tttgccgccg ggttcgatcc tggtggaggg tcccagtacg
      301 gtctccccag gcctcggaat gttcggcaag ctgaaaatca ggtcgagatt gatggacccg
      361 aacacgataa ccatcaggcg tctccctcct ggccggcgtc acgctgaacc agccgttctg
      421 ccggccggat gatcatggcg acggtgcctt gttgcccgcc tcgtccgggc gtccgcctgg
      481 atcggcaatc gcattgcagg caagcggcat cgggacggat ccggcgctac ggttgcgaaa
      541 ggaacgccct gacctggcgc aggaagaaat ccagctggtc atggtgtacc cagtgaccgg
      601 ccttctcgac cgccaggaca cttgcattgc tgaagtagtc taagcgcccg tcctccgccg
      661 ggttcttcgc ccagctttcg gtgccgtaaa ccagcagcgt cttgcaggtg atcgcgcccc
      721 atagctccgc gattgccgct cgggtcatgt catacggcgg ccaaagccgg acgtagtggt
      781 cgaacttcca gctataggtg ccgtcctcgt tctggttcac gccatactgc gtgagatgcc
      841 gggcctgttc gggcgtaaga tgcttgttct cctcctgcat gcgcgcatac gcagcctcca
      901 cggtcggata gcggcgcggt tgccggccag agtttgaccg ctgcgcatcg atccaggctc
      961 gcatgcgatc ggcgatcggt ttggtatcgg ccaccgcgtc ctgcccaggc cccggcccaa
     1021 ggccctcgat cgcgacgatg cggcgcacgt tctccggata gatgccggtg tagcgcagcg
     1081 cgatgttgcc gcccagcgag tgagcgatga tcgaaaccgg cgcgaggttc tgctgatgga
     1141 ccagttgtgc caggtcgtag atgaaattgg ccatggtata gtgaccggac gccgaccagg
     1201 cagaatcgcc atgtccgcgc agatcgggcg cgatcacatg gtagtcgcgg cgcaagtcgg
     1261 aggccaccca gtcccagttg cggcagtggt cgcgtccgcc atgcaacagg atcagcggcg
     1321 gtgcgccggc attgccccag tccacgtaat gcaggcgcag gcgctgcgag aagtaaatcc
     1381 gagaggtcgg accggattcg ggcaacatgt tcgatacctg tgttcagagc attgaccgcc
     1441 ggtgcacgtc cggcgtgcgc aattatgcta tcgcaaagcg ttatgccatt gccgaccgcc
     1501 tgtcagccca aaaacgctcg tccgcaccgt agcctggccg gcgtgacccc ttttggcccg
     1561 gccgcacatg ccaaggcgcg aattccttga agctgcctac caccatattg gtgtgaccgg
     1621 tggccatacc ccggcaatcg cgccgaaagg gcgtcacgac gcacgcccac cgtgcgagca
     1681 ggacacagca ggataacgcc gggccaatca tgatgccgac aggcaccgcc tgtcattggc
     1741 ggcattcgtc tcggcaaata ctgctcgggc gaaccatgcc cgcttcccgg ccgcccaaaa
     1801 tcacgttaat ccgcagccga tggctcggat tcgcatcctt ggagcgtcgg gctccggcac
     1861 ttcgacactc ggttcggcgc tggcaagtcg tctcggggtg ccgtacgccg attccgacag
     1921 cttctactgg ctggccaccg acccgcctta tacgacgccc cgtccgccgg aggatcggca
     1981 ggcgttgctg
//
LOCUS       DASWQN010000196         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_152776, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000196 DASWQN010000000
VERSION     DASWQN010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152776"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..1268
                     /locus_tag="VGC82_03225"
     CDS             384..1268
                     /locus_tag="VGC82_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0422314.1"
                     /translation="MGESEIDVLRTLLTSRPRPTSVSERRERLDALGAAYPTAGDIRL
                     EPVSVDGVPAEWSVAPGADDSRVLLYLHGGGFSVGSIVSHRAMVAEAGRAAQARTLAL
                     DYRRTPEHPFPAALEDALQAYRWLLAQGVQPSRIAIGGDSAGGGLTLAVMVSLREQGL
                     TLPACGWCISPWVDMEAQGESFATKAAIDPMISKDYILELAGWYLGGAASRTALAAPL
                     YADLVGLPPLLIQVGSAETLLDDSVKIAHRAGAADVRVTLEVWPNMIHAWPLFYQQLT
                     DGRQAIAAAGSFIRTATG"
     gene            1774..1938
                     /locus_tag="VGC82_03230"
     CDS             1774..1938
                     /locus_tag="VGC82_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422315.1"
                     /translation="MTEKVLWRFVTVKTLIRVAFAVVSLGGIAQAQTAGKSPPPQSGN
                     NYNFTNGGTG"
BASE COUNT          366 a          681 c          707 g          433 t
ORIGIN      
        1 gcttgttcgg cgagatcaac atgggttggt gtgatatcga cccacgttag agcagtttca
       61 cggtgactag aaactgggtt ctctcgcggc agcaaaggaa gtcgtgggtg gccccgccaa
      121 gcacggccat gacacatcgg cgtcgggcgg cgaaccggcc ggcaccttcc ggtcaccgtg
      181 aaactgccct aggatcggtt ccggtgcggc gccacctcgt catgccgacg aaggtcggca
      241 ttcacgcctt tcgcacgtat cgccaaagcc gcggatgatg acccttcggc agtcatgacg
      301 tcgcgagcct aatgcggatg tgtcgatctc tcggtggatt gctactatag gcctgtctgc
      361 cgcctctggg ggatttcaag acgatgggcg agagcgaaat cgatgtcctg cggacgctgc
      421 tgacgtcgcg gccgcgtccc accagcgtgt cggaacgccg ggagcggttg gacgcgcttg
      481 gtgccgctta ccccaccgcc ggtgatattc gtctggaacc ggtcagcgtc gacggcgtgc
      541 cggcggaatg gagcgtcgcg ccgggcgcgg acgattctcg ggtgctgctt tatctccacg
      601 gcggcggctt ctccgtcgga tcgattgtca gccaccgcgc gatggtggcc gaggccggcc
      661 gggcggcgca ggcccgtacc cttgccctcg actaccgacg cacaccggag catccgtttc
      721 cggcggcatt ggaggatgcg cttcaggcct atcgctggct gctcgcccag ggtgtgcagc
      781 ccagccgtat agcgatcggc ggcgatagcg cggggggtgg gttgaccctt gcggtcatgg
      841 tttctctccg cgaacagggc ctgacgttgc cggcgtgcgg ctggtgcatc tcgccgtggg
      901 ttgacatgga ggcgcagggc gagagctttg cgacgaaggc ggcaatcgat ccgatgatca
      961 gcaaggatta tatccttgaa ctggccggct ggtatcttgg cggggcagcg tcgcggacgg
     1021 cgcttgccgc cccgctttac gccgatctcg tgggtctgcc gccgctgctg attcaggtcg
     1081 gcagtgccga gacgctactg gacgattccg tcaagatcgc ccatcgggcc ggcgcggcgg
     1141 atgttcgggt cacgctggag gtctggccaa acatgatcca tgcatggccg ttattctacc
     1201 agcaactgac cgatggtcgc caagcgatcg ccgcggcggg atcgtttatc cgcaccgcga
     1261 caggctgact tgccggctgg tatgatcgcc attgcgcggt cttgccgctt gtatcccggc
     1321 tgcaccccgg tgccggccat ccgcaactat actgttaaga cacagtagtt ggtggctgcg
     1381 ccaagaaccg tcctgatact atcatccggt gcgtttcggc catcggccgg cgtggcggcg
     1441 ctgctggcag gcctcggggc ttgcggacaa cgatctgcga cggccagcga ggtcgccggg
     1501 aatgcggcgc attccgtagg cgtttctggg ccacctgatc cagcacatgc acattcgcgc
     1561 ggcccgagac cagaactggc cgctccgcgt gctccgcgcg gccgagcgga ttccatgggt
     1621 atttccacgc cacctaccac gttcgcctcc acggatggag gctacggtcc gggtcggcgg
     1681 tcatcgtgcg tgtgaccgcg gccactgcac tgtgacctga acaggcagat agatcaaatc
     1741 gcacaacggc aaccgataac aaaggagtat tccatgactg aaaaagtact ttggcgtttc
     1801 gtcacggtga agactttgat ccgtgtcgct ttcgccgtcg tcagcctggg cggaatcgcg
     1861 caagcgcaga ccgcgggcaa gtcgccaccg ccgcagagcg ggaacaacta taacttcacg
     1921 aatggtggca ccggctagtg tcgcgaagct ggggtccgcc atatccggcg gaccccagtc
     1981 tcgcgccact ctctctttgt ttcagtggcc tgctgattcc ctgcgtccgc cgcatgatgc
     2041 gacgaacttc gggggcagga caccagacca tttgcagcct gaccgtgcag gctgcgaatg
     2101 gtctatcttc ctgctttatg gcgtgattca cacgcttcga acgttccgcc cagcgtgacc
     2161 acgcgccaat ctgcttgcac cgagtcc
//
LOCUS       DASWQN010000197         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_548576, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000197 DASWQN010000000
VERSION     DASWQN010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548576"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..1352
                     /locus_tag="VGC82_03235"
     CDS             408..1352
                     /locus_tag="VGC82_03235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0422316.1"
                     /translation="MAKELRALANDVRVLELDGRLPYPDQAAIYAARTAWAALPADSV
                     KLIDGLALPAFDGLPLHQVTALIHHPASLETGVPEEIRQRLHATEAAMFRELPRLVVT
                     SDQTAERLAADFGAPRDRISVITPGIDDLPRCQGSAGPACQILSVGALIPRKGHDVLL
                     RALSRLFDLQWELTIAGSPARDSVHANGLHALAEQLRIAQRVRFVDDVDEALWARADL
                     FALTSYFEGYGVAIAEAMRRGLTVAVTNVGAVPALVGPEAGVVCSAGDVDQLSKALRR
                     LIFDRALLRDMAEVAWQSGRLLPSWNEQAGRLGVILAA"
     gene            1522..2790
                     /gene="otnK"
                     /locus_tag="VGC82_03240"
     CDS             1522..2790
                     /gene="otnK"
                     /locus_tag="VGC82_03240"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HEY0422317.1"
                     /translation="MLLGAIADDFTGATDLCSMLVRGGMRTVQLIGVPSSNLAVPEAD
                     AVVVALKSRTAPPGQAVEQSLAALRWLQAAGAKQFFFKYCSTFDSTDQGNIGPVADAL
                     TDALGCGFALACPAFPTNARTVYLGYLFAGGSLLNESGMEKHPLTPMTDANLVRVLSR
                     QTEGTVGLVPFATVDQGAHAIRDAMTQLKERGRRYAIVDAITDAHLVAIGEAAANHAL
                     ITGGSGVAMGLPANFRKAGLLSNATDPGALPKLDGASAVIAGSCSRATLAQLGYARSH
                     LPVFELDALGTPDAAALSEAALAWAEGKIGDKPVVIAASAPPEQVSVLQARLGREQAG
                     ALIEDAVARIAEGLVARGVRKMVVAGGETSGAVVGRLGVRSLRIGGEIDPGVPWTLAS
                     GSGSDLLLALKSGNFGAVDFFLKSFRVLEG"
     gene            2794..>3059
                     /locus_tag="VGC82_03245"
     CDS             2794..>3059
                     /locus_tag="VGC82_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0422318.1"
                     /translation="MSTTNAAERDTRRLLSELAAGLFARGFSVGSAGNISVRLTDGYL
                     MTPTNSSLGRLDPERLSKLDGEFRHVGGDKPSKEVFMHRAFYQAR"
BASE COUNT          509 a          934 c         1054 g          562 t
ORIGIN      
        1 gcaaaccggt agtattaagc gatccatcgc atgtgggcga tccatcgcat gtggacggct
       61 gttggcgtcg ctgccgaaca gcgtgttcag ttatcgtgac atagcgcgta accaacaaca
      121 cccggcgggc gaaggcaaaa gagtgtgatc gtgtgttgtc ctgccccaac ccgcgcgaga
      181 gtgttgttgc cggcagtgaa tcaagtctgg cctattcagg cgccatgacg ggaacggcgc
      241 gcgtgctgcg atttttctgc gatcggacat cgccccaatc gtgcccgaac tggtcatcgg
      301 tcgcgtgacg acgcgccaga acctgctaga atttcgatat gcaaatttgc ctgatcgttc
      361 cgggaccgct cgagaccgtt tccgggggcc atagctacga tcgccgtatg gcaaaggagc
      421 tgcgtgcgct ggcaaacgac gttcgcgtcc tggaactgga cggacgtctc ccctaccccg
      481 accaagccgc gatctacgcc gcccggacgg cctgggcggc tcttcccgcc gatagcgtga
      541 aactgatcga cggcctggcg ctgcccgcct tcgacggcct gccgctgcat caggtgaccg
      601 ccctgatcca tcatcctgcg tcgctcgaaa ccggcgtgcc ggaggaaatc aggcagcgcc
      661 tgcatgcgac cgaggccgcc atgttccgcg aactgccacg ccttgtcgtc accagcgacc
      721 agacggcgga gcggctggcc gcggatttcg gcgcaccgcg cgaccgtatc agcgtgatca
      781 cgccgggcat cgacgacctg ccgcgatgcc agggttccgc ggggccggcc tgccagatct
      841 tgtcggtcgg cgcgctgatt ccgcgcaaag gccacgacgt gctgttgcgt gcgctaagcc
      901 ggctgttcga cctgcaatgg gaattgacga tcgcggggtc gcctgcccgc gactcggtgc
      961 atgccaacgg tttgcacgcg ctggccgaac aactgagaat cgcgcagcgc gtccggttcg
     1021 tggatgacgt tgatgaagcg ttgtgggcgc gggccgacct gtttgccctc acgtcgtatt
     1081 tcgaaggcta tggagtggct atcgccgagg ccatgcgacg tggcctgacg gtggcagtga
     1141 ccaatgtcgg cgcggtaccg gcactggtcg gacccgaagc cggcgtggtg tgttcggcgg
     1201 gcgatgtcga tcagctttcg aaagcattgc gccggctgat cttcgaccgc gcgttgctgc
     1261 gcgacatggc ggaggttgca tggcagtccg gccggttgct gccgtcatgg aacgagcagg
     1321 ctggacgcct gggtgtcata ctggctgctt agcccggtag ctgctaatgt cctgctccga
     1381 agtttgtccc gtttgtgacg gacgcggcgg atgagcaggc cgccgaaagc aaagagcggg
     1441 cgtcctgcat cggaggttcg cccgctattg cgaacttccg aaccggatac tcgcgagacc
     1501 gaacacaggg agagccggac aatgctgtta ggtgcgatcg ccgacgattt cacgggtgcg
     1561 accgacctgt gttcgatgct ggtgcgcggc ggcatgcgaa cggtgcagtt gatcggcgta
     1621 ccctcgtcca atctggcggt gccggaggcg gacgcggtgg tggtagcact gaaatcgcgc
     1681 accgcgccgc cgggtcaggc ggtcgagcaa tccctcgcgg ctttgcgctg gctgcaagcg
     1741 gccggcgcga agcagttctt tttcaagtat tgctccacct tcgacagcac cgatcagggc
     1801 aatatcggcc cggtggcgga cgcactgacc gacgccctgg gctgcggttt cgcgcttgcg
     1861 tgcccggcgt tcccgaccaa cgcccgcacg gtctatctgg gctacttgtt tgccggcggc
     1921 agcctgttga acgaaagcgg catggagaag catccgctga caccgatgac cgatgcgaac
     1981 ctggtgcgcg tgctgtcgcg acaaaccgag ggcacggtgg ggctggtgcc attcgccacc
     2041 gtggaccagg gcgcccatgc gatccgcgat gccatgacgc agttgaagga acgcggccgc
     2101 cgctatgcca ttgtcgatgc catcaccgac gcgcacctgg tcgcgatcgg cgaggcggcg
     2161 gcgaaccacg cgctgatcac cggcgggtcc ggcgtggcga tgggattgcc ggcaaatttc
     2221 cgcaaggcag gactgctttc gaacgcaacg gatcccggcg cgctgccgaa actggatggt
     2281 gcgagcgcgg tcatcgccgg atcatgctcg cgggcgacgc tggcacagct tggctatgcc
     2341 cgttcccatc tgccggtgtt cgaactcgat gctttgggaa cgcccgatgc ggcggcattg
     2401 agcgaagcgg cgctggcgtg ggcagaaggc aagatcggcg acaagccggt ggtgatcgcc
     2461 gcgtccgcgc cgccggagca ggtcagcgtc ctgcaggccc gcctcggacg cgagcaggcc
     2521 ggggccttga tcgaggacgc ggttgcacgc atcgccgaag gcctggtcgc ccgcggcgtg
     2581 cgcaagatgg tcgtggccgg cggcgagacc tccggcgcgg tggtggggcg tctgggcgtg
     2641 cggtcgctgc gtatcggggg tgaaatcgat ccgggcgtgc cctggacgct ggcaagcggc
     2701 agcggctccg atctgctgct ggcgctgaaa tcgggaaact tcggtgcggt tgattttttc
     2761 ctgaagtcgt ttcgtgttct ggagggctga ccgatgtcga cgaccaatgc tgccgaacgc
     2821 gacacacgcc gcctgttgag cgagcttgcg gccggcctgt tcgcgcgggg attttccgtg
     2881 ggcagcgcgg gcaatatcag cgtgcgctta acggacggct atctgatgac accgaccaac
     2941 tcgagtctcg gccgactcga tcccgagcgg ctgtccaagc tggatgggga gtttcgccat
     3001 gtgggtggcg acaagccatc gaaggaagtg ttcatgcacc gcgcgttcta tcaggcgcg
//
LOCUS       DASWQN010000198         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2527891, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000198 DASWQN010000000
VERSION     DASWQN010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2527891"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1815)
                     /locus_tag="VGC82_03250"
     CDS             complement(55..1815)
                     /locus_tag="VGC82_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0422319.1"
                     /translation="MSTLRRFASYYRPHRRLFILDFGCAVLSGLLELAFPLAVRLFVD
                     QLLPGHDWRLISACAAGLMALYLINTGLMAIVTYWGHVLGIALETEMRRRAFEHLQRL
                     SFSFYDEQRTGHLIARVTKDLEDIGEVAHHGPEDLFIALMTFLGAFAMMFAVNIDLAL
                     LTVLIVPPILFVATSYGNRMTQNFRAIFARIGIFNARVEENIAGMRVVQAFANEAHER
                     RLFARDNDEYRRTKLQGYRLMAVSSALTYLSMRATQMIVMVAGTWFVLHGALSPGGFV
                     GFLLLVGVFFRPVEKISAVLETYPKGIAGFRRYCELIDMAPTLTDLPGAQPAPPLNGN
                     VAYEHVTFGYAPGRPVLRDVSLRVEAGETVAFVGSSGAGKSTLCALLPRFYDVQEGRI
                     TIDGIDIRSMTLESLRQQIGVVQQDVFLFAGTLRANIAYGRLGASESEIIEAADRARL
                     SAMIAELPDGLDTVVGERGVMLSGGQRQRVAIARMFLKNPPILILDEATSALDSETER
                     AIQQSLSELAAGRTSLIIAHRLATIRDADRIIVVDGRGVVEQGWHEDLLAADGAYRRL
                     HDVQFGAWDQTSAQPRASRL"
BASE COUNT          360 a          572 c          586 g          302 t
ORIGIN      
        1 catgctggga ggtgggtgga aatatgccgg gattgggcat ccagttttcg tccgttacag
       61 acgggatgcc cgcggttgcg ctgaagtctg gtcccatgcg ccgaactgca cgtcgtgcaa
      121 tcggcgatag gcaccgtcgg cggccagcag atcctcgtgc cacccctgct cgaccacgcc
      181 gcgaccgtca acgacgatga tccggtcggc atcgcggatc gtcgccagcc ggtgcgcgat
      241 aatcaggctg gtgcggccgg cggccaattc ggacagtgac tgctggatgg cccgctcggt
      301 ctcgctgtcc aacgcactgg ttgcctcgtc caaaatcagg atcggggggt tcttgaggaa
      361 catgcgggcg atcgccacgc gctggcgttg tcccccggac agcatcacac cacgctcccc
      421 aaccacggtg tcgagcccgt cgggcagctc cgcgatcatc gccgagagac gagcgcgatc
      481 ggccgcctcg atgatctccg actcgctcgc gccgaggcga ccataggcga tgttcgcccg
      541 cagcgtgcca gcgaacagga acacgtcctg ctgcacgacg ccgatctgct gtcgcagcga
      601 ctccagcgtc atcgagcgga tgtcgatgcc gtcgatggtg atgcgacctt cctgtacgtc
      661 gtagaagcgt ggcagcaagg cacacaatgt gctcttgccg gcgccggagg atccgacaaa
      721 agccaccgtt tcgccagcct cgacgcgcag gctcacgtca cgcagcaccg gtcgtccggg
      781 ggcatagccg aaggtgacgt gttcgtaggc aacgttgcca tttagcggcg gcgcgggctg
      841 ggcaccaggt aggtcggtca gcgtcggcgc catgtcgatc agttcacagt atcggcggaa
      901 gccggcgatc cctttgggat aggtctccag cacggcgctg atcttttcga ctgggcggaa
      961 gaacacgccc accagcagca ggaaaccgac gaacccgccg ggtgacagtg cgccgtgcag
     1021 gacgaaccat gtgccggcga ccatcacgat catctgcgtc gcacgcatcg ataggtaggt
     1081 cagagccgag ctcacggcca tcaggcggta gccctgcagc ttggtgcggc ggtactcgtc
     1141 gttgtcccgg gcaaacagtc ggcgttcgtg cgcctcgttg gcgaaggcct gaacgacgcg
     1201 catgccggcg atattctcct ccacccgagc gttgaagatg ccgatcctgg cgaagatcgc
     1261 gcggaagttc tgcgtcatgc ggttgccgta gctggtggcc acgaacagga tcggcgggac
     1321 aatcagcacg gtcagcaacg ccagatcgat gttgacggcg aacatcatgg cgaaggcgcc
     1381 gaggaacgtc atcagagcga tgaacaggtc ctcgggcccg tgatgggcca cttcgccgat
     1441 atcctccaga tccttcgtca cgcgggcgat caggtggccg gtccgttgct catcataaaa
     1501 cgagaacgac aggcgctgca ggtgttcgaa cgcccgccgg cgcatctcgg tctccagcgc
     1561 gataccgagg acgtggcccc aataagtgac gatggccatg aggccggtgt tgatcagata
     1621 aagggccatc agccccgccg cgcaggcgga aatcagacgc cagtcgtgcc ccggaagcaa
     1681 ctgatcgaca aacaaccgca cggcgagcgg gaatgccagt tccagcagcc ccgacagtac
     1741 cgcgcagcca aaatcgagaa tgaacagcct tcggtgcggc cgataatagc ttgcgaaacg
     1801 acgtagcgtg gacattgccg
//
LOCUS       DASWQN010000199         5920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2528033, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000199 DASWQN010000000
VERSION     DASWQN010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2528033"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            587..1453
                     /locus_tag="VGC82_03255"
     CDS             587..1453
                     /locus_tag="VGC82_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEY0422320.1"
                     /translation="MSIQNPVEWGVERLKLAFVKMESVNRGLLPAEQSIDSALPVIRR
                     IGITDLRDVLARGFDDFGANRTDVIFLCVFYPAIGLLLASLAAEHRMLPLLFPLISGF
                     ALVGPFAGVGLNEMSRRRELGIGKGWMDAFRVLQSPSIGAIFLLGLVLAGIHLAWLIV
                     AYTIYKATIGPQPLASANTFLHEVFLTTPGHQMIVVGIGAGFLFAVVVLVISSVAFPL
                     LLDRQVGIETAVKTSIQAARANPGPMAVWGMIIAAGLVIGSIPLFVGLIVILPVLGHS
                     TWHLYRKMVVPR"
     gene            1527..2345
                     /locus_tag="VGC82_03260"
     CDS             1527..2345
                     /locus_tag="VGC82_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY0422321.1"
                     /translation="MPIVIALVLIAVGSVAFHLINPWWLTPIASNWQYIDFTIAITFW
                     ITGFVFTAVVLFVAYCVFRFRHIEGRQAAYEPENKRLEWWLAIVTGVGVVAMLTPGLF
                     VWSQFVTVPPNATEFEAVGQQWSWGFRLPGKGGKLGASDAQYVSSDNPLGLNPDDPNG
                     KDNVVIVGDDMHLPIGKPVKVLLRSVDVIHDFYVPEFRAKMDLMPGTVTQFWFTPIRT
                     GTFEILCAGFCGVGHPQMRGKIVIDSESDYQQWLQKQHTFAQLSPPTKLAAKVR"
     gene            2451..4208
                     /locus_tag="VGC82_03265"
     CDS             2451..4208
                     /locus_tag="VGC82_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0422322.1"
                     /translation="MADVTADTVGSIGPAEVDEVELYHATTWVTKYVFSQDAKVIAIQ
                     YSMTATAIGFVALVLSWLMRLQLAFPGSFSLIDSTSYYQFITMHGMIMVIYLLTALFL
                     GGFGNYLIPLMVGARDMAFPYVNMLSYWIYLLAVLVLVASFFSPGGPTGAGWTLYPPQ
                     AILSGTPGHEWGIILMLASLILFIIGFTMGGLNYVVTVLQCRTRGMTLMRLPLTVWGI
                     VTATVMALLAFPALFVGCVMMTLDRLLGTSFFMPSMVELGEHLKYSGGSPILFQHLFW
                     FFGHPEVYIVALPAFGIVSDLISTHARKNIFGYRMMVWAIVIIGALSFVVWAHHMYIS
                     GMDPYFGFFFATTTLIIAIPTAIKVYNWVLTLWRGDIHLNPPMLFALGFIVTFVNGGL
                     TGLFLGNVVVDVPLSATLFVVAHFHMVMGVAPIFVVFGGIYHWYPKVTGRMLDNTMAK
                     VHFWVTFVGAYAIFFPMHYLGLLGVPRRYFELGETAFIPPSAASLNIFITIAALIVGA
                     AQLVFLFNLFWSVTRGKPAGSNPWRATTLEWQTPETPPGHGNWGKELPIVYRWPYDYS
                     VPGAKEDFIPQNQPAAAPS"
     gene            4358..5107
                     /locus_tag="VGC82_03270"
     CDS             4358..5107
                     /locus_tag="VGC82_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY0422323.1"
                     /translation="MIVIISFLAVVGVIMGWWLSHQRLMTKPWLEIDAFGDRPAWGGA
                     PIPVAKVGLGVFLAVVGCLFALLVSAYFMRIDLSRPGAVATAGVAAAAPVLRAMPIPK
                     LLWLNTAVLVVSSVALECAQIAGRRRQRDALQGALLIAAASTLVFLTGQLLVWRQLMD
                     AGYFVAGDPANAFFYLLTGVHGLHIAGGLVALGKTTAKVWRGIPIERLRVSMWLCTVY
                     WHFLLVVWLIIFAILTGWADDLIVICRSLVI"
     gene            5124..5861
                     /locus_tag="VGC82_03275"
     CDS             5124..5861
                     /locus_tag="VGC82_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III family protein"
                     /protein_id="HEY0422324.1"
                     /translation="MADLAPADLAPSVTGSAVRRAEGWEGFAADWSSDQRAFKTASWG
                     KVMMWIFLLSDTFIFSCFLLSYMTARMSTTVPWPNPSEVFALRLGGEPIPLILIAIMT
                     FVLISSSGTMAMAVNYGYRRDRSKTVALMLLTAAFGATFVGMQAFEWTKLIREGVRPW
                     GNPWGADQFGSCFFMITGFHGTHVTIGVIFLIITALKVRRGDYEHQRPGFFTSRKGRY
                     ENVEIMGLYWHFVDLVWVFIFAFFYLW"
BASE COUNT         1052 a         1748 c         1807 g         1313 t
ORIGIN      
        1 gaacgggtac tccatgatac cgacgccgac ggcgatattg cgtgccattg ctggcctccc
       61 tgttcctcaa ccgccggatc gtctctcgtt tcccagccag aggcaatccg atggcgtggc
      121 gcccgctgcc gccaatctca cgcgattggc gtcgcaccgc ggatccgtga gaccccggat
      181 tgccggtgcg cgcaaccgcg acatgcaaag gaactgcagg cagccggcaa cccccgtcgg
      241 aagcagagat ggatcgcgca agcagaccag gacatccggt gcaccgcatg aaccggagta
      301 gaccgaaccg gcgtttccag gcctgtgggc agtgcggtta cgccgttgcc cgagggggcg
      361 gcaacggccg atcaaggatg attcgtctct cagcggcgac cgcgaagtgg atgcgtggcg
      421 gcgccggatg accttggatt aaatgaccgt cataatgtcc ggtggacact cgggttgaaa
      481 aagactacca attgcaatca agcggggctt gggactctcg tggattgact cggatacacg
      541 ggtagcctgc gaccccgtcg aaccaacaag gttcgggagg aacggcatgt ctattcagaa
      601 ccccgtagaa tggggcgtgg aaaggctcaa gctggcattt gtaaagatgg agtccgtcaa
      661 tcgcggcctc cttcccgcgg aacaaagcat tgattcggcg ctgccagtca ttcgccgcat
      721 cgggatcacg gatcttcggg acgtgctggc ccgcgggttc gacgacttcg gggcgaaccg
      781 gaccgatgtt attttccttt gcgttttcta cccagcgatc ggattgttgc tggccagtct
      841 ggcggcggaa cacagaatgc tgccgctgct gtttccgctc atttccggct ttgccctggt
      901 cggaccgttc gccggtgtcg gcctcaatga aatgagccgg cggcgggagt tgggcatcgg
      961 taaaggttgg atggatgcgt tccgcgttct gcaatcgccg tcgataggcg cgatttttct
     1021 gcttggcctg gtactggctg ggatccatct ggcctggctg atcgtggcat acacgatcta
     1081 caaagcaacg atcgggcccc agccgcttgc ctcggcgaat actttcttgc acgaggtttt
     1141 cctgacaacg ccgggccacc agatgatcgt cgtcggcata ggcgccggat tcttatttgc
     1201 cgtggtcgtg ctggtgatca gctccgtcgc atttccgctg ctgctcgatc ggcaagtggg
     1261 gatcgaaacc gcggtaaaga catcgatcca ggcggcgcgg gccaaccccg gccccatggc
     1321 cgtgtgggga atgatcatcg ccgctggcct ggtgatcgga tcgattccct tgttcgtcgg
     1381 tctcatcgtc atcctgccgg tgctcgggca ctccacctgg catctgtacc gcaagatggt
     1441 ggtgccgcga tgaactgcgt ttcgcgtcgc gtagcgtcga gggcctgccg gcggcaggat
     1501 atcgcagagc caaaggtagg tgacaaatgc caatagtaat cgcgctcgtc ttgatcgctg
     1561 tcggttcggt ggcgtttcac ctgataaacc cgtggtggct gacgccgatc gcctccaact
     1621 ggcagtacat cgacttcacc atcgccatta ccttctggat taccgggttc gtcttcaccg
     1681 ccgtcgtttt gttcgtcgct tactgcgtgt tccgcttccg tcatatcgaa ggacgccagg
     1741 cagcctacga gccggaaaac aagcggctgg aatggtggct ggcgatcgtg acaggcgtgg
     1801 gcgtcgtggc catgctcacc cccggcctgt ttgtatggag ccagttcgtc acggtcccgc
     1861 cgaacgcaac cgagttcgag gcggtgggtc agcaatggag ctggggcttc cggctgccgg
     1921 gaaagggcgg caaactcggc gcgtcggatg cccaatacgt cagctccgac aatcccttag
     1981 gtctcaatcc cgatgatccc aacggcaagg ataatgtggt gatcgtcggc gacgacatgc
     2041 atctgccgat cggcaagccg gtcaaggtcc tgctgcgttc cgttgacgtc atccatgatt
     2101 tctacgtgcc cgagttcagg gccaaaatgg acttgatgcc gggcacggtc acgcagttct
     2161 ggttcacccc catcaggaca ggaacattcg aaatcctttg tgccggattc tgcggggtgg
     2221 gacacccgca aatgcgcggc aagatcgtga tcgacagcga gagcgactat cagcaatggc
     2281 tgcagaaaca gcacaccttc gcccaattat cgccgccaac caagctggcg gccaaggtaa
     2341 gatgaagcag ggccttggca tagcagctcc cgtccgggag agtggaaatc ccagcattgg
     2401 atagatcatg ctaatgaatg gtatcaggaa cgagcgagga gtgcagtttc atggctgacg
     2461 ttactgccga cactgtcggc tccattgggc cggcggaagt cgacgaagtc gagctttatc
     2521 atgcgacaac ctgggtaacg aaatacgtct tcagccagga tgccaaggtc atcgccatcc
     2581 aatattcgat gacggctacg gccattggct tcgtggcctt ggtgctgtca tggctgatgc
     2641 ggctgcaact ggcgttcccc ggctcgttct ccttgatcga cagcaccagt tactaccagt
     2701 tcatcaccat gcacggcatg atcatggtga tctacctgct gacggcgttg ttcctgggtg
     2761 gcttcggcaa ctaccttatt ccgctgatgg tcggcgcccg ggatatggcg tttccttatg
     2821 tgaacatgct gagctactgg atctacctac ttgccgtgct ggtcttggtg gccagcttct
     2881 tttcgccggg tggccccacc ggcgccggct ggacgttata tccaccgcag gccatcctgt
     2941 ccggaacacc cgggcacgaa tggggcatca tcctgatgct cgcctcgctg atcctgttca
     3001 tcatcggctt caccatgggt ggcttgaact acgtggtgac ggtgttgcaa tgccgaaccc
     3061 gcggcatgac gctgatgcgc ctgccgctga cggtctgggg catcgttact gcgacggtga
     3121 tggcgctgct ggcgtttccg gcgttgttcg tgggctgcgt gatgatgacg ctggacaggc
     3181 ttctgggcac cagcttcttc atgccgtcca tggtcgaact tggcgagcat ctgaaataca
     3241 gcggcggcag cccgatcctg ttccagcatt tgttctggtt cttcggccac cccgaggttt
     3301 acatcgtcgc tctgccggcc ttcggcatcg tctccgacct gatcagcacc cacgcgagga
     3361 agaacatctt cggctaccgg atgatggtgt gggccatcgt gatcatcggt gcgctcagtt
     3421 tcgtggtgtg ggctcaccac atgtacatca gcggcatgga tccctatttc ggattcttct
     3481 ttgccacgac cacgctgatc atcgcgattc ccaccgccat caaggtttat aactgggtac
     3541 tgacactgtg gcgtggcgat atccacctta acccgccgat gctgttcgcg cttggcttta
     3601 tcgtcacgtt cgtgaatggc ggcctgaccg gcctgttcct tggcaacgtg gtcgtcgatg
     3661 tgccgctttc ggcgactctg ttcgtcgtcg cccactttca catggtgatg ggtgtggcgc
     3721 cgatcttcgt ggtgttcggc ggcatctacc actggtaccc caaggtgacc ggccggatgc
     3781 tcgacaacac gatggccaag gtgcacttct gggtcacctt cgtgggtgcc tacgcgatct
     3841 tctttccaat gcactatttg ggtttgctgg gtgtgccccg ccgctatttc gaactcggcg
     3901 aaaccgcctt cattccgccg tcggccgcgt cgctgaacat cttcatcacc attgccgcct
     3961 tgatcgtggg cgccgcccag cttgtcttcc tgttcaacct gttctggagc gtcacccggg
     4021 gcaagccggc cggcagcaat ccatggcggg ccacgacatt ggagtggcag acgcctgaaa
     4081 caccgcctgg acatggcaac tggggcaagg aactgccgat cgtttatcgt tggccctacg
     4141 actacagcgt gcccggcgcc aaggaagatt tcatcccgca gaaccaaccc gctgccgcgc
     4201 cgagctagac ggtggctgac acatccgacc atgtgcggcc tgatgttcgg cgagcaactt
     4261 cgtcgccccg gtgccgcaca gcggttccgg ccggggcggc ggccagcgcg gcgccccgat
     4321 catgtcggcg gcttatgtca tgccgggaat ttgtatcatg atcgtcatca ttagttttct
     4381 cgccgtggtc ggggttatta tggggtggtg gctgtcacac cagcggctga tgaccaagcc
     4441 ctggctggaa atcgacgcgt tcggcgaccg gcccgcgtgg ggcggagcgc ccatacccgt
     4501 tgccaaggtc ggattgggcg tgtttctggc cgtcgtcggc tgccttttcg cgctgctggt
     4561 cagtgcctat ttcatgcgga tagacctgag cagacccggt gcggtcgcga ctgccggcgt
     4621 agctgcggct gctccggtac tgcgcgccat gccgatcccc aagctgctat ggctgaatac
     4681 cgcggtgctg gtggtgagca gcgtagcgct ggaatgtgcc caaatcgcgg gccgacgccg
     4741 tcaaagggac gccttacagg gtgccctgct gatagcggca gcatccaccc tggtgttcct
     4801 gacggggcaa ttgcttgtct ggcgtcaact gatggatgcc gggtatttcg ttgccggcga
     4861 tcccgcgaat gccttctttt acctgctgac cggggtgcac ggcctgcata tcgcgggcgg
     4921 actcgtggct ttgggaaaga ccaccgccaa ggtctggcgg ggcatcccga tcgagcgact
     4981 gcgagtaagc atgtggttgt gcacggttta ctggcacttc ctgctcgtgg tttggctaat
     5041 cattttcgcc atactgacag gctgggcaga cgacctcatc gtcatctgcc gaagcctggt
     5101 catctagagg ggatcatcag ccgatggcag accttgcgcc agcagacctt gcgccaagtg
     5161 taacgggaag cgccgtcagg cgggctgaag gctgggaagg cttcgccgcc gactggtcgt
     5221 ccgatcagcg ggcgttcaag accgcgtcgt gggggaaggt catgatgtgg atcttcctcc
     5281 tgagcgacac gttcatcttc agctgtttcc tgctgtcgta catgacggcg cggatgtcga
     5341 cgaccgtgcc gtggcccaat cccagtgaag tgttcgcgct aagactgggc ggcgaaccca
     5401 tcccgcttat tctgattgcc atcatgacgt tcgtcctgat cagcagcagt ggcaccatgg
     5461 cgatggcagt caactacggt taccgccgtg accggtccaa gaccgtcgcc ctcatgctgc
     5521 tgaccgcggc gtttggcgcc acgttcgtcg ggatgcaggc attcgaatgg accaagctga
     5581 tcagggaagg cgtccggcca tggggcaacc cgtggggtgc ggaccagttc ggctcgtgct
     5641 ttttcatgat caccgggttc cacggcaccc atgtgacgat cggcgtgatc ttcctgataa
     5701 tcaccgcgct caaggtgcgg cggggcgatt acgaacatca gcggccgggt ttcttcacca
     5761 gcaggaaagg ccgctacgaa aacgtcgaga tcatgggcct gtactggcat ttcgtcgatc
     5821 tcgtctgggt gttcatcttt gcatttttct atctctggtg aggcacgaat gtcacacgct
     5881 gcaactcatg cgacagcaac tcccgtaagt gcccatcacg
//
LOCUS       DASWQN010000200         3704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1868523, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000200 DASWQN010000000
VERSION     DASWQN010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868523"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VGC82_03280"
     CDS             <1..747
                     /locus_tag="VGC82_03280"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY0422325.1"
                     /translation="LMAAALADGRTVLANAAREPEIGDLADCLVKMGARIEGIGSDKL
                     TIDGVDSLHGAEHAIIPDRIETGSYACAAAISGGSVFLRDSRLDHLGATVRILREAGV
                     DIAEEPDGLMIRRLNGLHGADVMTEPYPGFPTDMQAQFMALMSVAEGACMVTETIFEN
                     RFMHVPELNRMGARINVYGASAIVRGVPSLSGAPVMATDLRASLSLILAGLAAQGETI
                     VNRVYHLDRGYEAVEQKLAACGADIERISG"
     gene            759..1295
                     /gene="ppa"
                     /locus_tag="VGC82_03285"
     CDS             759..1295
                     /gene="ppa"
                     /locus_tag="VGC82_03285"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY0422326.1"
                     /translation="MRIDAITIGKNPPEDLNVIIEVAIGGEPIKYEMDKEAGTLVVDR
                     FLYTPMRYPGNYGFVPHTLSDDGDPIDVLVANTRPIVPGAVINVRPVGVLKMTDDGGG
                     DEKIIAVPSPKLTQRYVNVRNYTDLPSITLEQIQHFFEHYKDLEPGKWVKVEGWGDAA
                     EAKTLIMQAIERARQAKI"
     gene            complement(1354..2214)
                     /locus_tag="VGC82_03290"
     CDS             complement(1354..2214)
                     /locus_tag="VGC82_03290"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HEY0422327.1"
                     /translation="MRESAMPPIVAVIAPGMMGSAVAGRLTASGCQVRTSLAGRSDAS
                     IARARAAGMTDATDAQLAEADFILSIVPPGQALALAERLSGAMRAAARKPIYVDCNAV
                     SPDTVLRIDRVVRETGATFVDGGIIGPPPETDSSRTRIYLSGPDAEKVVVLGQYGLTT
                     PVQPGPVGAASAMKMSYAGITKGFTALGATMMLAATRAGTADALVAEMKVSQPALLKW
                     LTAQMPKMHSKAYRWVAEMEEIAAFVGPDQTGDGFYQSAARLYQRIAADFDGPNVETG
                     ALDAFCDKAE"
     gene            complement(2418..3158)
                     /locus_tag="VGC82_03295"
     CDS             complement(2418..3158)
                     /locus_tag="VGC82_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY0422328.1"
                     /translation="MAINNAYDDGDDFLLNMTNRGRGRPSRRRGFDDDFSFDRAPPGD
                     MPRAAPSWPSSAGFGPEHDATVKWFNPEKGFGFVALSDGSGDAFLHANTLNQSGHSAV
                     SPGATLRVRIGQGQKGRQVSEVLSVDESTATPTAGRAAGAGAGGAPRPAGGFGDRAAG
                     GPAGFGGAPRRGAPTGPAVEMQGIVKWYNATKGFGFVAPSEGGKDVFVHASALQRANV
                     MQLAEGQTIWMDVVQGAKGPEAASIRVD"
     gene            complement(3498..>3704)
                     /locus_tag="VGC82_03300"
     CDS             complement(3498..>3704)
                     /locus_tag="VGC82_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422329.1"
                     /translation="PALLKVYGDLGLTALPLGPDGLPMPESEGDTPPAEQYLVCRAER
                     DLHLLLPVLPALANGPRAGVAAVV"
BASE COUNT          712 a         1225 c         1138 g          629 t
ORIGIN      
        1 ctgatggcgg cggcgctggc ggatggccgc acggtgctgg ccaacgcggc gcgtgagccg
       61 gagatcggcg acctggcgga ttgcctggtg aagatgggcg cgcggatcga aggcatcggc
      121 agcgacaaat tgaccatcga cggcgtcgat agcctgcatg gcgccgaaca cgccatcatc
      181 ccggaccgca tcgaaaccgg cagctacgct tgtgccgccg ccatctccgg cgggtccgtg
      241 ttcctgcggg actccaggct ggatcacctg ggtgcgacgg tgcgtatcct gcgtgaggcc
      301 ggcgtggaca tcgccgagga acccgacgga ctgatgatca ggcgcctgaa cgggctgcac
      361 ggcgcggatg tgatgaccga accttacccc ggcttcccga ccgacatgca ggcgcagttc
      421 atggcgctga tgtccgtcgc tgaaggcgct tgcatggtca ccgagacgat cttcgaaaac
      481 cgcttcatgc atgtcccgga attgaaccgt atgggcgcgc ggatcaacgt ctatggcgcc
      541 tcggcaatcg ttcgcggcgt gccgtcgttg tccggtgcgc cggtcatggc caccgacctg
      601 cgtgcgtcgc tgtcgctgat cctggccggc ttggccgccc agggcgaaac catcgtcaac
      661 cgcgtctatc atctcgaccg cggctatgag gcggtcgagc agaaactggc ggcctgtggc
      721 gcggatatcg aacggatctc tggttagggg ataaacctat gcgtatcgat gccatcacca
      781 tcggcaagaa cccgcccgaa gacctgaacg tcatcatcga ggtcgccatt ggcggcgagc
      841 cgatcaagta cgagatggac aaagaagccg ggacgttggt ggtggaccgc ttcctctaca
      901 cgccgatgcg ctatcccggg aattacggct tcgtgccgca caccctgtcg gacgacggcg
      961 atccgatcga tgtgctggtg gccaacacca ggccgatcgt acccggtgcg gtgatcaacg
     1021 tccgcccggt cggcgtgctg aaaatgaccg acgacggcgg cggagacgaa aaaatcatcg
     1081 ccgtgccctc gccgaagctg acgcagcgtt atgtcaacgt ccgcaactat accgacctgc
     1141 ccagcatcac gttggagcag atccagcatt tcttcgagca ctacaaagac ctggaacccg
     1201 gcaaatgggt aaaggtcgag ggctggggcg acgcagccga ggcgaagacg ctgatcatgc
     1261 aggcaatcga acgggcgcgg caggcaaaga tctgattgtc ccggttgggc cggcgatcac
     1321 cgtcgcccgc ggcaggcgat cacccgccct gccctattcg gccttgtcgc agaacgcgtc
     1381 cagcgcgccg gtttcgacat tggggccatc gaaatctgcc gcaatcctct ggtataaccg
     1441 agcggcggat tgataaaagc catcgccggt ttggtccgga ccaacgaacg cggcgatctc
     1501 ttccatctcc gccacccagc gataagcctt gctgtgcatc ttcggcatct gggccgtcag
     1561 ccacttcaat aaggccggct ggctgacttt catctccgcc accagcgcgt cggccgtacc
     1621 ggcccgtgtg gcagccagca tcatcgtggc acccaatgcg gtgaatccct tggtaattcc
     1681 cgcatacgac atcttcatcg cggaagccgc tccgaccggt cccggctgca ccggggtcgt
     1741 caacccatat tgccccagca ccacgacctt ctcggcgtcc ggccccgaaa gatagatccg
     1801 cgtcctgctc gagtccgtct cgggcggtgg gccgatgatc ccgccatcga cgaaggtcgc
     1861 accggtttcc cgcacgaccc ggtcaatccg cagcacggtg tccggactta cggcattgca
     1921 gtccacatag atcggtttgc gggcagccgc gcgcatggcg cccgatagcc gctccgccag
     1981 tgccaaggcc tgaccggggg gaacaatgga caggatgaaa tccgcctccg ccagttgcgc
     2041 atcggtggcg tccgtcatgc cggccgcccg cgcccgcgca atggaagcat cagaacggcc
     2101 ggcgagcgat gtccgaacct gacaaccgga agcggtcaga cgcccggcca cggcgcttcc
     2161 catcatcccc ggcgcgatca cagcaacaat cggtggcatg gctgactccc tcatacacgg
     2221 accagaatgc accacggtgt cgaaccaggg aagacaaccc ccgcactgat aacagcgacc
     2281 atcttcggcg ccggcccccc ctgaggtctg cttgcacggg aggcaaaata cctgggagac
     2341 aaatcacacg ggaggcaaca aaaaacccgg cacgatctgc tcgcgccggg tctttttctc
     2401 agcccatcga cagaacctca gtccacgcgg atagaagccg cttcggggcc cttggcaccc
     2461 tggaccacat ccatccaaat cgtctggcct tcagcgagct gcatcacgtt cgcccgctgc
     2521 agtgcggagg catgaacgaa gacgtccttg ccgccctcgg acggtgccac gaagccaaag
     2581 cccttggtcg cattgtacca cttcacgata ccctgcattt cgacagcggg accggttgga
     2641 gcgccgcggc gcggggcgcc accgaatcct gcgggaccgc cggcggcgcg atcaccgaat
     2701 ccacccgcgg gtcgcggggc gccacccgcc ccagcgccag cggcgcgtcc ggccgtcggc
     2761 gtcgcggtgc tttcgtcaac cgacagtacc tcggaaacct ggcgcccctt ttggccctgg
     2821 ccgatgcgca ccctaagagt ggcgcccgga ctgaccgcac tgtggccgga ttggttgagg
     2881 gtgttggcgt gcaggaatgc atcgcccgaa ccatcggaca gcgccacgaa gccaaacccc
     2941 ttttcggggt tgaaccactt gacggtcgcg tcatgttcgg gaccgaaccc tgcggaggag
     3001 ggccacgacg gtgcggcccg cggcatgtcg cctggcggag cacgatcgaa gctaaaatcg
     3061 tcgtcgaagc cgcggcgccg gctcgggcgt ccgcggccgc ggttggtcat gttcaagaga
     3121 aaatcgtccc catcatcata ggcgttgttg atggccactg cagccctcga ggggcgtggt
     3181 agccggtttg ttcctgttcg aatagacgaa cctgaggagc atggcagctc cccaactacc
     3241 gaatatcgta acagctcatc atgtaagccg attcactata ccgcttgagc gggcgggcgc
     3301 cagagcgaat cgcgcagaaa ctcgataaac accaatgtta ctctggatgt tgtcaagccc
     3361 gctcttgctc gtgaagcgaa agaattcact tgacgccttg attcggcttg gtttccgaca
     3421 gcggaaagcg gcttgtccca gccgactgct ccagcaaatt ccacggccgc accccgcacg
     3481 gttggcggtt cgtgccttca gaccacggcc gccacgccag ctcttggccc gttcgcgagc
     3541 gccggcagca ccggcagcag cagatgcaag tcccgttcgg ccctgcacac taaatattgc
     3601 tcggcaggcg gcgtgtcgcc ctccgattcg ggcatcggca ggccgtccgg tcccagcggc
     3661 aaagcggtca ggccgagatc gccgtaaact ttcagcaggg cggg
//
LOCUS       DASWQN010000201         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1340440, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000201 DASWQN010000000
VERSION     DASWQN010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340440"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VGC82_03305"
     CDS             complement(<1..627)
                     /locus_tag="VGC82_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0422330.1"
                     /translation="MIIDCHGHYTTAPKQHEQWRQQQIEAHAKGAQAPVRPLITDDEI
                     RDSIRNGQLRMQRERGTDLTIFSPRAAGMAHHLGDAGTSEAWAIACNDLIHRVCSLFP
                     DNFVGVCMLPQSPGISPKNSIAELERCVGSHGFIGCNLNPDPSGGYWTDPPLSDPYWY
                     PIYEKLVELDVPAMIHVSQACNPAFHTTGSHYLNGDTAAFMQFLTSDVF"
     gene            620..1078
                     /locus_tag="VGC82_03310"
     CDS             620..1078
                     /locus_tag="VGC82_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422331.1"
                     /translation="MIIVFQPSRAALSPVVFQNPAQLNSRDHGKQGARRRLQTEMLQE
                     CNTCGQYTSVLITFERVDEALPPPAVGWLGVEVHRSIRCQGRADMPAFIQHHQGENAP
                     IICPGCVGLLPMYVREVEPHWSMAKIDFIYECSDCGAEVRQTITRPEQRH"
     gene            1385..>1576
                     /locus_tag="VGC82_03315"
     CDS             1385..>1576
                     /locus_tag="VGC82_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422332.1"
                     /translation="MLEINKPRSRKPADGGLTGQVRVRGAREHNLKSIDLDIPRDALV
                     VFTGVSGSGKSSLAFGTLYA"
BASE COUNT          306 a          469 c          489 g          312 t
ORIGIN      
        1 gaagacgtcc gaggtgagaa actgcatgaa ggccgctgtg tcgccgttga gatagtgcga
       61 gccggtggta tggaacgccg gattgcaggc ctggctgaca tggatcatgg cgggaacgtc
      121 gagctcgacc agcttttcgt agatcggata ccagtaggga tcggaaagcg gaggatcggt
      181 ccagtatccg ccggaagggt ccggattcag gttgcatcca atgaagccgt ggctgccgac
      241 gcagcgctcc agctcggcaa tcgaattctt gggcgaaatg cccggactct gcggcagcat
      301 gcagacgccg acgaaattgt ccggaaacag actgcagacc cggtggatca ggtcattgca
      361 ggcgatggcc cacgcttcgc tggttccggc atcgccaagg tgatgtgcca tgccggcagc
      421 gcgcggcgag aaaatggtca gatccgtacc gcgctcgcgc tgcatccgaa gctgaccatt
      481 gcggatcgaa tcccggatct cgtcatcggt gatcagaggc cgaaccggcg cttgcgcgcc
      541 cttcgcatgg gcctcgatct gctgctgtct ccactgctca tgctgcttgg gggccgtcgt
      601 atagtgaccg tggcagtcga tgatcattgt attccaaccc tcgcgcgcgg ctttgtcgcc
      661 ggtcgtcttc cagaacccgg cgcagttgaa ctctcgagat cacgggaagc aaggggcgcg
      721 gcggcgtttg cagaccgaaa tgttacagga atgcaacacc tgcggacaat atacgtctgt
      781 tctcattacg ttcgaaagag ttgatgaggc gctgccgccg cccgcggtag gctggctcgg
      841 ggttgaggtt caccgcagca tccgctgcca agggagagcc gacatgccag ccttcattca
      901 gcatcaccaa ggcgagaacg ctcccatcat ctgccccggc tgtgtgggac tgcttcccat
      961 gtatgtcagg gaagtcgagc cccattggag catggcgaag atcgacttca tctatgagtg
     1021 ctcggactgc ggcgccgagg tcaggcagac catcacaaga ccggaacagc ggcattaagg
     1081 cattcggtcc cgccgcccca tcggcgggcg ccttggccag ccgcctacgt tcaacaattt
     1141 cacggccatg gctctgaaat gagccgcaat ctctgatgcg ccaaaaggct tttcgacgcc
     1201 tcgcactcgc gtcgcgcgcg aagtactgcc aagcttccgc gaacggccgt ctcatgatcc
     1261 cggcctcctg atcgttcccc tgcagctcat gtcccgtaac cgggctgctg cggcgtgacg
     1321 gctgtcattg ttttcggaac ccggcccggt tctggtgcgt ttacctttgg ccggatcatg
     1381 aaaaatgctg gaaattaaca agcctcgttc tcgaaagccg gcggatggcg ggctaacggg
     1441 acaggtccgc gtgcgcggag cgcgcgagca caatctcaag agcatcgatc tcgacattcc
     1501 cagggatgcc ctggtggtgt tcaccggcgt gtccggctcg ggaaaatcgt cgctggcctt
     1561 cggcacgctc tatgcg
//
LOCUS       DASWQN010000202         4210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2132949, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000202 DASWQN010000000
VERSION     DASWQN010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2132949"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VGC82_03320"
     CDS             complement(<1..902)
                     /locus_tag="VGC82_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422333.1"
                     /translation="MGHVSRRAILAGAATATVLQRPAIAQPAPLKVGMMLPFSGTYAA
                     LGENIAASFELLVAENGGKLAGRPITLIRLDDESDPSKAPANVNRLIERENVDVLLGT
                     VHSGVVMALVQAAREKEIPLFIPNAGNVAATRDLCSVSVFRSSFSNWQPAYGMGRVLA
                     ARGVKKAAWVTWDYAAGRESGEGFRDGLRAGGAEMTHLLTVPFPETNFQPILAQIGGL
                     GVEAVGSFFAGGGAIQFVKDYAASGLRDKIPLCGSGFLTEGVLQPQGAAAEGIQTALH
                     YGDGLDNPKNTAFRAVFKAKTGRDA"
     gene            997..1473
                     /locus_tag="VGC82_03325"
     CDS             997..1473
                     /locus_tag="VGC82_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY0422334.1"
                     /translation="MSEVFSTPPQDRFFEDYVVGATYVCGSFKLSEDEIIAFASRYDP
                     QMMHIDRDLAASGPFGRIIASGWHTIAAAMRLMVDNFLPHNGLAAPGIDELRWPRPVR
                     PNETLTLHATVQEARRSRSKPDRGLIRTLLEVLNQNGEVVMSMKPLNMVRVRNPEI"
     gene            complement(2098..4041)
                     /locus_tag="VGC82_03330"
     CDS             complement(2098..4041)
                     /locus_tag="VGC82_03330"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY0422335.1"
                     /translation="MAGWKDKLALTAQKNRQDIVKAKLSRREMMRLGLLTTGGGLIVK
                     KGLSARAFADDGSLNFVKGVDGPPSPPARPWAQPMPILPVKTAVDPFNASLMPGGPPD
                     GTTLIDGATQRVPHQLFTFDAQTRTYGGNFPPQKFYRLNMQEVHVKLHPDYEATTVWG
                     FDGQVPGPNFHAIYGEPILVRFQNSLPSVKVPQNFGIAEMTTHLHNGHTPTESDGNPV
                     DFFNSVNDTGPLEKDANGKLIRVNPGGFKDQHYPNVYAGYTPTKNPTNPNSFVPANQN
                     SIATPAGDKTEALSSLWYHDHHLEFTAQNVYKGMFGCYNLFDALDTGNETTGLRLPSG
                     AYDIPIFFNDFLFDADFQLVFDLFDLDGILGDRFCANGAIQPFLNVDKRRYRFRLYNP
                     GPSRWYQFALFDGSNFVPFWQISTDGNLLPQAVQVDSVRLSVAERIDIIIDFGKINAS
                     RLYIVNRLEQVNGRGPTGNVLTPGTPIVQLNLGAPKPDASRDPADGKPFALRPLPDAD
                     FNALLQRAAKAKTRTWRFERGNGAWQVNGELFDPDVISASIEQGSEEVWVIQNPGGGW
                     RHPIHAHFEEHRVLTQDGAPIKPNTQVNGTIDYARRDVLPLNTNNEVRTFRRFRDMKG
                     RYVMHCHNVVHEDHAMMIRFDIV"
BASE COUNT          814 a         1290 c         1264 g          842 t
ORIGIN      
        1 gcatcgcggc ccgtcttcgc tttgaacacg gctcggaagg cggtgttctt ggggttgtcc
       61 aggccgtcgc cgtagtgcag agcggtctgg atgccctcgg cggcggcacc ttgcggctgc
      121 agcacaccct cggtcaggaa tcccgaaccg cagagcggaa ttttgtcgcg cagccccgac
      181 gcggcgtagt ccttgacgaa ctggattgcg ccgccgccgg cgaagaagga tccgacggcc
      241 tcgacgccca ggccgccgat ctgcgccagg atcggctgga agttggtttc cgggaacggc
      301 accgtcaaca ggtgcgtcat ctctgccccg ccggcacgca aaccgtcgcg gaagccttcg
      361 ccggattcgc ggccggcggc ataatcccag gtgacccagg ccgctttctt gacgccgcgg
      421 gccgcgagca cccgacccat gccgtaagcc ggctgccagt tgctgaacga cgagcggaag
      481 accgacaccg aacacaggtc gcgggttgcc gccacgttgc cggcgttggg gatgaacagc
      541 gggatttcct tctcgcgcgc cgcctgcacc agggccatga ccacgccgga gtggacggta
      601 ccgagcagga catcgacgtt ttcgcgctcg atcaggcggt tgacgttcgc gggcgccttg
      661 gagggatcgg attcatcgtc cagccggatc agggtaatcg gccggccggc gagcttgccg
      721 ccgttttcgg ccaccagaag ttcgaacgaa gcggcgatgt tttcgccgag ggcggcgtag
      781 gtgcccgaaa acggcagcat catgcccact ttcagcggcg ccggctgggc gatcgccggg
      841 cgctgcagta ccgtcgccgt ggctgccccg gccagtatgg cgcgccgcga aacgtgaccc
      901 atccttatcc ccctggttac aagcccttgc ggctattgat tgtcgtgcga catatcgcat
      961 gttaccgggc gaaacggcaa cagagcggta cgatcgatgt cagaggtttt ttccacacca
     1021 ccacaggacc gcttcttcga agactatgtg gtcggcgcca cctatgtttg cgggtccttc
     1081 aaactgtccg aggacgagat catcgccttc gcttctcggt acgatccgca aatgatgcac
     1141 atcgatcgcg atctggccgc cagcggcccc ttcggcagga tcatcgccag tggctggcac
     1201 accatcgccg ccgccatgcg gctcatggtc gataacttcc tgccgcataa cggactggcc
     1261 gcgccgggaa tcgacgaact gcgctggcct cgcccggtcc gccccaacga gaccctcacg
     1321 ctgcatgcga cggtccagga ggcccggcgg tcgcgcagca aaccggatcg tggattgatc
     1381 cgcacgcttc tggaggtgct gaaccagaac ggcgaggtgg tcatgagcat gaaaccactc
     1441 aacatggtgc gggtgcgcaa cccggaaatt tagagcgtca tcgcgctgac cggacgtgtt
     1501 ggacatcccg attccgaaag catacccggg gccgatgcgg cgggcgcgtg cgttcactcg
     1561 ttcttacttt caacacggag gaagaacgag tcccacgaag cgccacggag caggattgtg
     1621 ccgcaatacc gggagccagc ggacgcgatc gatttggcaa tcggggctaa ctggattcca
     1681 atgcgattgg acctgcccgc cgattttccg cgcgactggc gacagtgacg acgtttcgcc
     1741 ggtagtaata aaccgcgtta agatcgaccc tctggccgcg ccacctcgtc atgccaacga
     1801 gggtcggcat ccacgccttc cgcacgtatg accaacaccg tggctggcgg ctcttcgacc
     1861 accgcggcct gaaggctgat gcggacgggt caaactttcg gcggatccgt atgagacgcc
     1921 tcagtggcga acggccaacg gccagaacgc gatgcaatca cccacatccg tacttggcgt
     1981 tctgaccgca acgttccggc cactagccac cgggacccat tccgtgccga tccgtgcgct
     2041 ctcccgcttc tccgtgatga gacctacgag ccggacaacc catccgcatc cctccgtcta
     2101 aacgatgtcg aatcgtatca tcatcgcatg gtcttcatgc acgacattat ggcagtgcat
     2161 gacatatcgc cctttcatgt cccggaagcg ccggaaggtc cggacctcat tgttggtatt
     2221 gagcgggagc acgtcccggc gagcgtagtc gatggtgccg ttgacctgtg tattgggctt
     2281 tatcggcgca ccgtcctgcg tcagcacgcg atgttcttca aagtgagcat ggatgggatg
     2341 ccgccaccca ccgccaggat tctggatcac ccagacctct tcggagccct gctcgatgct
     2401 ggcgctgatg acgtccggat cgaaaagttc accgttaacc tgccaagcgc cattgcctcg
     2461 ctcaaaccgc catgtcctgg tcttcgcctt tgccgcgcgt tgcaacaggg cattgaagtc
     2521 agcatcggga agcggccgca atgcgaatgg cttgccatcc gcgggatcgc gacttgcgtc
     2581 cggcttaggc gcaccaagat tgagctgcac gatcggcgtt ccgggggtca ggacgttacc
     2641 ggttggcccg cggccgttta cctgttccag ccggttgacg atatacagcc gagatgcatt
     2701 gatcttgccg aagtcaatga tgatatcaat gcgttccgcg acgctcaggc gcacgctatc
     2761 aacctgcacg gcctgcggca gaaggttgcc atcggtcgat atctgccaga acggcacgaa
     2821 gttcgagccg tcgaacagcg cgaactgata ccagcgcgag ggtccgggat tgtatagccg
     2881 gaagcggtag cggcgcttgt ccacgttcag gaacggctgg atcgctccgt ttgcgcaaaa
     2941 tcggtcgcca aggatcccat caagatcaaa caggtcgaac acaagttgaa agtcggcatc
     3001 gaacaggaaa tcgttaaaga agatcggtat gtcatacgcc ccgctcggca gacgcaggcc
     3061 agtggtctcg ttacccgtat ccagggcatc gaacaggttg taacatccga acatgccctt
     3121 atagacgttc tgggccgtga attcgagatg atggtcgtgg taccaaagcg aacttagagc
     3181 ttcggtcttg tcgcccgccg gcgtggcgat gctgttttga ttagccggaa caaacgaatt
     3241 cgggttcgtt gggtttttcg tcggcgtata gccggcataa acgtttggat agtgctgatc
     3301 cttgaagcct cccgggttga cgcggatcaa cttgccattc gcgtcctttt caagcggccc
     3361 cgtgtcgttg accgagttga aaaaatcgac cgggttgcca tcactctcag taggggtgtg
     3421 accgttgtgc aaatgggtcg tcatctccgc gatgccgaag ttctgcggca ccttgaccga
     3481 cggcaggctg ttctggaacc gcaccaggat cggctcgcca tagatggcat gaaagttggg
     3541 tcccggcacc tgtccgtcga aaccccagac ggttgtcgcc tcgtagtcgg gatgcagctt
     3601 gacgtgcacc tcctgcatat tgagccggta gaatttctgc ggtggaaagt tgccgccgta
     3661 tgtccgggtc tgagcatcga acgtgaagag ctggtgcggg acccgctggg tcgccccgtc
     3721 gatcaaggtg gtaccgtcgg ggggaccgcc cggcatcaaa ctggcattga acggatcgac
     3781 agcggtcttg accggcagaa taggcattgg ctgcgcccac ggcctcgcgg gcgggctggg
     3841 cggtccgtcc acgcctttga cgaaattgag gcttccgtcg tcggcgaagg cccgggcact
     3901 aaggcccttc ttgacgatca ggccaccgcc ggtcgtcaac aggcccagac gcatcatctc
     3961 cctgcggctc aacttggcct tgacgatatc ctgccggttt ttctgagccg taagagccag
     4021 tttgtccttc caacccgcca ctgtcccatc tcctgatttg ttttgaataa gtaatatgat
     4081 atcaagcggc ggccctcggc gatccacccg aacccgaggc tcggacgcat tgcaaacatc
     4141 agcagtctat attaagggaa ttgcccgcta ttttgcctgt aatggtatct atttcaaaat
     4201 tatgcttgcg
//
LOCUS       DASWQN010000203         3461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2132996, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000203 DASWQN010000000
VERSION     DASWQN010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2132996"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1154
                     /locus_tag="VGC82_03335"
     CDS             219..1154
                     /locus_tag="VGC82_03335"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0422336.1"
                     /translation="MKIGFIGLGMMGSGMASNLQKAGHDLVVHDLTRQAASRHLNAGA
                     TWAESPRALAEACDIVFTSLPTPADVQKVGTGDNGLIEGFRKGSVWFDLSTNAVDVVR
                     SLHATFADKGIDFLDGPVSGGPKGANSGRLAIWVGGDKAIFDKYLPALSAMGDQAVYI
                     GPIGAGSIAKLVHNASSAAVNVVLSEVFTMGIKAGVEPLALFEAVRQGASGRNRMFDR
                     LADHFLTGSYDPADFALRLLHKDVTLACQLAREVQVPMRLTNLALMELTEALNRGWGA
                     RDSRVGSLLQQERAGIPPIAVAPEKIKAVLEADVK"
     gene            1211..2110
                     /locus_tag="VGC82_03340"
     CDS             1211..2110
                     /locus_tag="VGC82_03340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY0422337.1"
                     /translation="MQLGALVPFTDTGGDPSLVRDFAQTLEAQGYDFLEAPDHVLGAS
                     PGKNADADARRTIADDMFHDPFVLLGFLSAATTKLRFSTGVLIVAQRQTALVAKQAAC
                     LDVLSGGRFRLGIGVGWNPVEFTALNENFHNRGRRSEEQAQVMQALWAQPHVTFKGKY
                     HTIDDAGINPRPASGRIPLWYGGHHEHTLPRIAKWGDGWMPNAYPPDQSALDVFSELR
                     RLTEQAGRDPAAIGIEVWTSCGEGSEADWRRETTFWKQAGVSHICLTTTFDRRHHHRI
                     PGRTVADHLAATKRYHDAIADLL"
     gene            2134..3030
                     /locus_tag="VGC82_03345"
     CDS             2134..3030
                     /locus_tag="VGC82_03345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY0422338.1"
                     /translation="MLLGATIPVADIGTGPAEIRDYAQTVEGLGFDYLQAPDHVLGAN
                     PATIAGKGRIGTNENPYHDPFVLFGFLAGCTQKLGFAPGVLILAQRQAALVAKQAACL
                     DVLSNGRLRLGVGVGWNKVEFTGLNEVFSNRGRRSEEQVQVMQALWSQPHVNFKGRYH
                     TIDDAGINPRPASGHIPVWFGGHAEATLQRTAKYGDGWMPLAYPADDTALAVFEKLNG
                     MIKAEGRDPASVGLEVWQSLGSGDEAAWYRDFAFWKKAGVTHVTAHTTYAMGHHKRIA
                     GRTAADHLAAITRFKQVVADLL"
     gene            complement(3087..>3461)
                     /locus_tag="VGC82_03350"
     CDS             complement(3087..>3461)
                     /locus_tag="VGC82_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422339.1"
                     /translation="FRHCRNSSDAAASPPDTTACPGFCPCYLKVGLFINTQYSRDVDV
                     PAQIPALVQQVKVARDAELASLWFPHHWLTKPMQMQRIMPVMTYFASWRGPLHHAQPV
                     PGLEQEKVLGSLRRFGEAVIPE"
BASE COUNT          666 a         1151 c         1022 g          622 t
ORIGIN      
        1 ccaaagcggc aacttcgaag tacgctccaa caccgatccc gatggcgact gcatatttca
       61 aggcattacc cccgaccgca gctttggcgc gaagcgcaat atctgttcct ccgtgaccct
      121 tcgcggccct cgttcttcct ccgtgttaaa aattacttgc ttgcccgcgg cagccaccag
      181 ttcccactat cgagccaacg cacataaaga ggaaacaaat gaaaataggc ttcatcggac
      241 taggcatgat gggctccgga atggcgtcca acctgcagaa agctggccat gaccttgttg
      301 tccacgacct gacccgccag gcggcatccc ggcacctgaa cgccggcgcc acctgggccg
      361 aaagcccgcg cgcgctggcc gaggcctgcg acatcgtttt cacctcgttg ccgacccctg
      421 ccgacgtgca gaaagtcggc accggcgaca atggcctgat cgagggcttc cgcaaaggct
      481 ctgtctggtt cgatctctcg acgaatgctg tcgacgtcgt gcggtctctc catgccacgt
      541 tcgcggacaa aggtatcgac ttcctggatg ggccggtcag tggcggtccg aaaggcgcca
      601 acagcggtcg cctcgccatc tgggtcggtg gcgacaaggc gatattcgac aagtacctgc
      661 cggctttgtc cgcgatgggc gatcaagccg tctatatcgg tccaatcgga gcgggatcga
      721 tcgccaagct ggtgcataat gcctcctcgg cggcggtcaa cgtggtgctg tccgaagtat
      781 tcaccatggg catcaaagcc ggtgtcgagc cgctcgcgct gttcgaagct gtccgccaag
      841 gcgcctccgg ccgcaaccgt atgttcgatc gtctggcgga ccatttcctc accggcagct
      901 atgatcccgc cgacttcgcc ttgcgcctgc tccacaagga cgtgacgttg gcctgccaac
      961 tggcacgcga ggtccaggtg ccgatgcgct tgaccaacct ggcactgatg gaactgaccg
     1021 aggccttgaa ccggggctgg ggtgcccgtg attcccgggt gggttcgctg ctgcagcagg
     1081 aacgcgcggg cattccgccg attgccgtgg ccccggagaa gatcaaggcg gtgctggaag
     1141 ccgacgtaaa gtaggcgcca gcgtgcgaag tttttgaagc atcaccgata ctcgaaagga
     1201 tcctcctccc atgcagctcg gagcgctcgt tccctttacc gacacaggcg gcgatccgtc
     1261 cctggtccga gacttcgccc aaacccttga agctcagggc tacgacttcc tggaagcgcc
     1321 cgaccatgtg ctgggcgcga gccccggaaa gaacgccgac gccgatgccc gccgcacaat
     1381 cgccgacgac atgttccacg acccattcgt gctgctgggc ttcctgtccg ccgcgaccac
     1441 gaagctgcgg ttctccaccg gcgttctgat cgtcgcgcaa cgccagactg ctctcgttgc
     1501 caagcaggca gcctgcctcg acgtgctttc cggtggccgc tttcgcctgg gaatcggcgt
     1561 tggctggaac ccggtcgagt tcaccgccct gaacgaaaac ttccacaacc ggggccgccg
     1621 ttcggaagaa caggcccagg tcatgcaggc gctgtgggct cagccgcatg tgaccttcaa
     1681 gggcaagtat cacaccatcg acgatgccgg catcaacccg cgcccggcat ccggccgcat
     1741 cccgctctgg tatggcgggc accacgagca caccttgccg cgcatcgcca aatggggcga
     1801 cggctggatg ccgaatgcct atccgcccga ccagtccgca ctcgatgttt tcagcgagct
     1861 gcgccgcctg accgaacagg ccggccgtga tcctgccgcc atcggcatcg aggtctggac
     1921 ctcctgcggc gagggttccg aagctgactg gcgccgtgaa acaacgttct ggaagcaggc
     1981 cggcgtcagc catatttgcc tgaccacgac tttcgaccgg cgccaccacc accgcattcc
     2041 gggacggacg gtagccgacc atctcgcagc gacgaaacgc taccacgacg ccatcgccga
     2101 cctcctttaa gccccaatcg gaaagaaacg aaaatgctgc taggcgcaac gatcccggtc
     2161 gccgatatcg gcaccggtcc cgcggagatc cgcgactatg ctcaaacggt tgaaggactg
     2221 ggcttcgact acttgcaagc ccccgaccat gtgctcggcg ccaatcccgc gacgatcgcc
     2281 ggcaaggggc ggataggaac caatgaaaat ccctaccacg atcctttcgt cctgttcggc
     2341 ttcctcgccg gctgcaccca gaagctcggt tttgctcccg gcgtcctgat cctggctcag
     2401 cgccaggctg ccctggtcgc caaacaggcc gcttgcctcg acgttctgtc gaacggccgc
     2461 ctacgcctcg gggtgggcgt cggatggaac aaggtggagt tcaccggcct gaacgaggtc
     2521 ttctccaacc gcggccgccg ctccgaagaa caagtgcagg tcatgcaggc gctgtggtcg
     2581 cagccgcatg tcaacttcaa aggccggtac cacactattg acgacgccgg catcaatcca
     2641 cgccctgcct ccggccatat ccccgtctgg ttcggcggcc acgcggaggc aacattgcag
     2701 cgcaccgcca aatacggcga cggctggatg ccgcttgcct accccgccga cgacaccgcg
     2761 ctggcggtgt tcgaaaagct gaacggcatg attaaagccg agggccgcga ccccgccagt
     2821 gtcggcctgg aggtttggca atcgctcggt tccggagacg aggctgcctg gtaccgcgac
     2881 ttcgccttct ggaagaaggc cggcgttacc catgtgaccg cccacactac ctatgccatg
     2941 ggccaccata agcggatcgc cggacgcacc gcggcggatc acctcgcggc gatcacgcgg
     3001 ttcaagcagg tggtggctga tctactgtag gttgcgtcgg catcgaagtc tcatcgtgct
     3061 ggcttgtgga cgatgggatc ttgggtctat tccggaataa ccgcttcgcc gaaccgccga
     3121 agcgacccaa gaaccttctc ctgctccaga cctggcactg gctgcgcatg atgaagtggc
     3181 ccacgccaag aagcgaaata cgtcatcacc ggcatgatcc gctgcatctg catcggtttg
     3241 gtcagccagt gatgcgggaa ccaaagcgac gccagttcgg cgtcccgagc gaccttgacc
     3301 tgttgcacaa gcgccgggat ctgtgccggc acatcgacgt cccgggaata ttgcgtgttg
     3361 atgaaaagcc cgactttcag gtaacagggg caaaaccccg ggcatgccgt ggtatcaggc
     3421 gggctggccg cggcatcgga cgagttgcgg caatggcgga a
//
LOCUS       DASWQN010000204         4032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_549543, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000204 DASWQN010000000
VERSION     DASWQN010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549543"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..283)
                     /locus_tag="VGC82_03355"
     CDS             complement(17..283)
                     /locus_tag="VGC82_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422340.1"
                     /translation="MGTDVTEVLTRVDWQVVAPNGQGSFHPTNIDTMRTLDPDVLIFS
                     DPAMRQTLRPSEAWKPVRAVREGRALVAPSLPSVGSRSRLRSIG"
     gene            245..469
                     /locus_tag="VGC82_03360"
     CDS             245..469
                     /locus_tag="VGC82_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY0422341.1"
                     /translation="MDPGEHFRHVGAHHPAHAHTAPSGGATTSHVMIEVSPTVSITAS
                     IANEAAEELGVKPGMQAIAVIKASDVMVGV"
     gene            complement(642..1076)
                     /locus_tag="VGC82_03365"
     CDS             complement(642..1076)
                     /locus_tag="VGC82_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HEY0422342.1"
                     /translation="MTPVALYPDKQQGQAMSIFASIMEKIFHAPQADKPAASPETAMS
                     PPSEVPPSTPGTAAGKIDVADVLTQKASEKGGGGNWQTSIVDLLKLLDLDSSLTARKE
                     LASELNVRAGADGSAEENIALHKAVMLKLAENGGTVPDSFKG"
     gene            complement(1286..2125)
                     /locus_tag="VGC82_03370"
     CDS             complement(1286..2125)
                     /locus_tag="VGC82_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY0422343.1"
                     /translation="MDIAQQIGLSPADSLVRRYGVAATPDVGPWNEHISLLLSHRSVR
                     GYRPDALPVGTLETLIAAAQSAATSSNLQTWSVIAVTNPDKKAALAKVAAGQKHIEQC
                     PLFLVWVADISRNQRLGVEEGVTLETMPYQETFLVAAIDAALAAQNATVAAESLGLSS
                     VYIGALRNDPPEVARIVGLPSGAFGVFGMCVGYASAAGATEVKPRQPQDVDVHHETYD
                     ATNETAHRAAYDALLQSFSTRHGMVMDTWTKRVLGRMGKIAAMSGRDKLVATLKAMGF
                     ELK"
     gene            complement(2284..2790)
                     /gene="bamE"
                     /locus_tag="VGC82_03375"
     CDS             complement(2284..2790)
                     /gene="bamE"
                     /locus_tag="VGC82_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEY0422344.1"
                     /translation="MPNMSRHLILIACVLLSSCAWLMPPPQVRGNKVDPESLKELVPG
                     TSTKADVTAVIGSPTAHDTFDDNTWLYISELTQQRIGRTLGELQQDVVVLNFNESGVL
                     AGINKVDKDAALPVTVVDRTTPSPGTEASFLQQLLGNIGRFSPAGASPGTGTGGTGGG
                     AGGAPATR"
     gene            2907..3464
                     /locus_tag="VGC82_03380"
     CDS             2907..3464
                     /locus_tag="VGC82_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEY0422345.1"
                     /translation="MAGLLRRRGKRERAAFQLYAAAVAAARDPYFYATLGVPDTLDGR
                     FDTIGLHVYLTIRRLNDKPDPGAAAESGQAMAQAVFDAMFLDMDINLREMGVGDMSVG
                     KRNRAMWEAFHGRSAAYAAAWDDPAALEVALARNLWRGAEPPDGAAGAMARVAYAQSM
                     YLENQSMGAMLLGDVRFLPAEEAAR"
     gene            3461..3946
                     /locus_tag="VGC82_03385"
     CDS             3461..3946
                     /locus_tag="VGC82_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007283576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEY0422346.1"
                     /translation="MSPEFHRPVSLDRIGPHGLDLTIEATAAECAALASRMDVPAVLA
                     MSCTFHLIREGRDNVLARGALHAKVTQTCVISLEDFDSLIEEVFQVRFVPSGEESDDI
                     DPESDDEIPFEGNAVDLGEAAAEQLGLALPPYPRMPGVEMPESEDEPQTHPFAALRRL
                     N"
BASE COUNT          721 a         1280 c         1287 g          744 t
ORIGIN      
        1 agccatgcca gtcctatcag ccgattgatc gaaggcggct cctcgaccca accgaaggca
       61 ggcttggcgc gacgagggca cggccttctc gtaccgcacg cacgggtttc catgcctcgg
      121 acggtcgaag agtttggcgc atggccgggt cggagaatat cagcacatcg ggatcgagcg
      181 tgcgcatcgt gtcgatgttg gtcggatgga agctgccctg tccatttgga gcgaccacct
      241 gccaatcgac ccgggtgagc acttccgtca cgtcggtgcc caccacccag cccacgcgca
      301 tacagcacct tcgggtggcg ccaccacgag tcatgtgatg atcgaagtat cgccgaccgt
      361 gtcgatcact gcttccatcg ccaacgaggc cgccgaggaa cttggagtga agcctggtat
      421 gcaggcgata gcggtgatca aggcatccga cgtgatggtt ggcgtgtgat accggctcgc
      481 cctgacaggg ccgttaacca gcatgaccga gaacggcgcg caaccgcgtc gctgggccgg
      541 ttgcagacct cgcgacgcgg cgtgcggggc agtagggtgc gcagggcatt catcataagc
      601 cggccatcat aagccggcgc taacctgatc ttttcgatga ttcaaccttt gaagctgtcc
      661 ggaaccgttc cgccgttttc tgccaacttc agcatcacgg ccttatgcaa ggcgatattt
      721 tcctcggcgc tgccatccgc gccggcgcgg acattcagtt cgcttgcgag ttcctttctc
      781 gccgtcaggc tggaatcgag gtccagcagc ttcaacagat cgacgatcga ggtctgccag
      841 ttgccgcctc cgcctttctc cgaggccttc tgcgtcaaca cgtccgccac gtcgatcttg
      901 ccggcggcgg tgcccggagt ggacggcggg acctcggacg gcggagacat tgccgtctcc
      961 ggcgatgcag ccggcttgtc cgcttgtggc gcatggaaga tcttttccat gatcgatgca
     1021 aaaatactca ttgcctgtcc ttgctgcttg tccggataca gcgcgacagg cgtcaagcag
     1081 ttccccgcgg gtgcccaacc ggtgccagtc agggcgacca aacgaggatc tggatcagct
     1141 tggggtccgc tccgaaacaa ccgcttcggg tgcggtctcg gataccgtat ggatgggcga
     1201 aggccaccac ggcgttgctg gggaagttcg cccggttcga agcgattatc taccaccagc
     1261 cagtcagcgt ggccggccgc gagacctact tcaattcaaa ccccatcgcc ttcaacgtgg
     1321 cgaccagctt gtcgcgcccc gacatcgccg cgatcttgcc catgcggccg agaacccgct
     1381 tcgtccaggt gtccatcacc atcccatgac gtgtcgagaa cgactgaagc agcgcgtcgt
     1441 acgccgcccg gtgagcggtc tcgttggtcg cgtcataggt ttcgtggtgc acgtccacgt
     1501 cttgcggctg gcgcggcttc acctcggtcg caccggcggc cgacgcgtag ccgacgcaca
     1561 tgccgaacac cccgaaggcg cctgacggca ggccgacaat gcgggcgacc tccggcgggt
     1621 cgttgcgcag cgcgccgata tagaccgagg acaagcccag cgattcagcg gcgaccgtcg
     1681 cgttctgcgc cgccagcgcg gcatcgatgg cggccaccag gaacgtctcc tgatacggca
     1741 tcgtctccag tgtgacccct tcctccacac cgaggcgttg gttgcgtgaa atgtccgcga
     1801 cccagaccag gaacagcgga cattgctcga tatgtttctg gcccgccgcc actttagcca
     1861 gcgccgcctt cttgtcggga ttggtaacgg cgatcaccga ccaggtttgc aggttggaac
     1921 tcgtcgccgc cgactgcgcc gcggcgatca gggtttccag ggtgccgacc ggcagcgcat
     1981 cagggcgata gccgcggaca gaccggtgcg aaagcagcag cgagatatgt tcgttccacg
     2041 gaccaacgtc cggagtggcc gcgacgccat agcggcggac cagcgagtcg gcgggcgaaa
     2101 gaccgatctg ttgcgcgatg tccatgatga gcgacctgtt ccttgtggca ggacgcggcc
     2161 actcggaccc cgggtcaggt caagggcagg cgtccattcc ggatggcgag gaggctgccc
     2221 gacgccgcgg ttcctgacaa gaccgtcatc ttgtgacaga ccgtcatccg gcggcggcgg
     2281 ccgtcatcgc gtggccggtg cgcccccggc accgccgcca gtgcccccgg tgcccgtccc
     2341 cggcgaggcg cctgccggac tgaagcggcc gatattgccc agaagttgct gcaggaatga
     2401 ggcttcggta cccggcgacg gcgtggtacg atctaccacc gtaaccggca gcgccgcgtc
     2461 cttgtcaacc ttgttgatcc cggccagcac gccgctttcg ttgaaattca gcaccacgac
     2521 gtcctgctgc agttcaccca gggtacgtcc gatgcgctgc tgggtcagtt cgctgatata
     2581 cagccaggtg ttgtcgtcga aggtatcgtg cgcggtggga gagccaatca ccgcggtcac
     2641 gtcagccttc gtggacgttc cgggaaccag ttccttcagc gattccgggt caaccttgtt
     2701 gccgcgaacc tgcggcggcg gcataagcca agcgcaggac gacagcagca cacaggcgat
     2761 gaggatcagg tggcgtgaca tatttggcat aggcactggt gcatcccggg atttctgccg
     2821 atcggctggt aggatcggcg cgcagtatta tctatatctg cctgtcacgg ccggcccggc
     2881 cgtgtcaatg acatcatgag gcagaattgg ccggtctcct tcgccgacgc ggcaagcgcg
     2941 aacgtgccgc atttcagctt tacgccgccg cagtcgccgc tgcgcgcgat ccctatttct
     3001 atgctacgct cggtgtgccc gacacgctgg acgggcggtt cgacacgatt ggactgcatg
     3061 tttatctcac gattcgccgc ctgaacgaca aacccgaccc gggggccgcg gcagaatccg
     3121 gacaggcaat ggcgcaggcg gtattcgacg ccatgttcct ggacatggac atcaacctga
     3181 gagagatggg cgtgggtgac atgtccgtcg gcaagcgcaa ccgagcgatg tgggaggcct
     3241 tccatggccg cagcgctgcg tatgcggcgg catgggacga tccggcggcg ctggaggtgg
     3301 cgctcgcccg caacctttgg cgcggagccg aaccaccgga cggagcggcc ggcgcgatgg
     3361 cgcgcgtcgc ctatgcccag tcaatgtacc tcgagaacca gtccatgggc gccatgctgc
     3421 tgggcgatgt cagatttctg ccggcggagg aggccgcgcg atgagccctg aattccaccg
     3481 tcccgtctcg ttggaccgga tcgggccgca tgggctggac ctgacgatcg aggcgaccgc
     3541 cgccgaatgc gctgcacttg cgtcgcgaat ggatgtgccg gcggtgctgg cgatgtcatg
     3601 cacattccac ctgatccgcg agggaaggga caacgtgctg gcgcgtggtg cgcttcatgc
     3661 caaggtgacg cagacctgcg tcatcagcct ggaggatttc gactcgctga tcgaggaggt
     3721 tttccaggtc cgttttgttc catccggcga agaatcggac gatatcgatc cagaatcgga
     3781 tgacgaaatt cccttcgagg gcaatgctgt cgatctcggc gaggcggcgg ccgagcaact
     3841 cggcctggcg ctgcctccct atccgaggat gccgggtgtc gagatgccgg aaagcgagga
     3901 cgaaccgcag acgcatccat tcgccgcgtt acgccggttg aactgaaagt tcgccagcct
     3961 gaagcgggtt cgccagcccc tgaacatgcg gcccctgaac atgcggcccc tgaatatgcg
     4021 gtccctggac at
//
LOCUS       DASWQN010000205         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2396811, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000205 DASWQN010000000
VERSION     DASWQN010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2396811"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="VGC82_03390"
     CDS             <1..1375
                     /locus_tag="VGC82_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY0422347.1"
                     /translation="SVYENFQRLVLPYSTGNLHPRFMGWVHGGGSPVGMLAELLAGGL
                     NANLGGRDHAPVEVERQVISWAAEMLGFPEAASGVLVTGTSVANLIGVLVARSAILGP
                     AVRSLGLDGARLVAYCSAAAHGCLPRAMEMAGLGRDALRMVPCDEHGRIRLEELARRV
                     AADRAAGARPFLVVGTAGTVDIGAIDDLGGIADFAAAEGLWFHIDGAFGAIAALSPKL
                     RPLLAGIERADSVAFDFHKWAQVPYDAGCIVVRDGSRQLDAFGAEAAYLRREQRGLAG
                     GGIWPCDLGPDLSRGFRALKVWMTLSVYGADRIGAIAEQTCTLAAILAARVSREPALE
                     LLAPVALNIVCFRYVAASGDLDRLNADITADLQVAGIAAPSTTIVNGVLAIRAAIVNH
                     RTNEADIAVLVDAVLAAGRMRTAQKEVPPAIHRGQVYREASRLGDAGTRGPFPTACAG
                     TLAAVRP"
     gene            complement(1589..>2330)
                     /locus_tag="VGC82_03395"
     CDS             complement(1589..>2330)
                     /locus_tag="VGC82_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HEY0422348.1"
                     /translation="HRHARGLILTEQGEALNRTVREVFAKLAITEALLTESKEKPAGR
                     LKITTTVGFGSSWLAPRLQGFLEAYPDVSISLLLDDTELDLAMREADVAIRMHPPKQP
                     DLVQRHLLTMQWNVVAAPEYLKKHGVPQRAEDLDAHRLVLFGQHHPPVQEVNWLADTG
                     RRPGSPRRALLEVNSIHAVLLAIRSGMGIGALPDYVVNENPDLVHVLSDLKGPKVDVF
                     FVYPEELRNSKRVAVFRDFLLARLAEMH"
BASE COUNT          383 a          744 c          775 g          428 t
ORIGIN      
        1 ctcggtctat gagaatttcc agcgcctggt gctgccctac tcgaccggca acctgcatcc
       61 gcgcttcatg ggttgggttc atggcggcgg cagtccggtc ggcatgttgg cggagctgct
      121 ggccggcggc ctgaacgcaa acctcggcgg ccgcgatcat gcgccggtcg aggtggaacg
      181 acaggtcata tcctgggcgg cagagatgct gggattcccc gaagctgcct ctggcgtgct
      241 tgtgaccgga acctcggtcg ccaacctgat cggcgtgctg gtcgctcgat ccgctatcct
      301 gggcccggcc gtgcgcagtt taggtttgga cggcgcgagg ctggtcgctt attgctccgc
      361 cgcggcacat ggctgcctgc cccgggcgat ggagatggca ggcctcgggc gcgacgcgct
      421 gcgaatggtc ccttgcgatg agcacggacg gatcaggttg gaagaactcg cacgtcgggt
      481 tgcggcggat cgcgcggcgg gtgcgcgccc gttcctggtc gtcggcaccg cgggcacggt
      541 ggacatcggc gcaatcgacg atctgggcgg aatcgcggac ttcgccgccg ccgagggtct
      601 gtggtttcac atcgacggcg cattcggggc catcgccgcc ctttcgccga agctgcggcc
      661 gctgctggcc ggtatcgaac gtgccgactc cgtcgccttc gatttccaca aatgggcgca
      721 ggtgccatac gacgccggct gcatcgtggt ccgggacggt tcacggcagc tcgatgcctt
      781 tggcgccgag gcggcttatc tgcggcgaga acaacgcggg ttggcaggcg gcggaatctg
      841 gccatgcgat ctgggaccgg atctgtctcg cggattccgc gcgctgaagg tgtggatgac
      901 gttgtcggtg tatggcgccg acaggatcgg cgccatcgcg gagcagactt gcacgctggc
      961 cgcgatcctg gcggcgcggg tgagccgcga gccggcgctg gaactattag cgccggtcgc
     1021 gctgaacatc gtctgctttc gctatgtggc cgcgtcaggc gacctggacc gactgaatgc
     1081 cgacataacc gccgatttgc aggtcgccgg tatcgccgcg ccatcaacta ctatcgtaaa
     1141 cggcgtgctg gcgattcggg cggcgatcgt gaatcatcga accaacgagg ccgacattgc
     1201 ggtgctggtg gatgcagtgc tggcagccgg ccgcatgcgt acggctcaaa aagaagtgcc
     1261 cccggctatt caccgggggc aagtatacag ggaggcttca cgtctgggag acgcagggac
     1321 cagaggcccc tttccaacgg cttgcgctgg aactctggcc gcagtgcgac cgtaactggt
     1381 ggctgcttgt ctatcccgcg gatgggaacc gaaccattgt ccagaaccgg caccgggcat
     1441 gctggcaccg cataccagcc cgcgcgtgcc accgaaaatg gcatggctgg gtcccgctta
     1501 aggcggacct ggacaatgat ccggtgatcg tgccccccgt tcgtcacccg ccctgttcgt
     1561 cgcccgccct gttcgtcgcc tactttggtc agtgcatctc cgcgaggcgg gcaagaagga
     1621 aatcgcggaa caccgcgacg cgcttggaat tacgcagttc ctcgggatag acgaagaaca
     1681 cgtccacctt ggggcccttc aggtccgaca gcacatggac caggtccggg ttctcgttca
     1741 ccacgtagtc cggcagcgcg ccgatcccca tgccggagcg aatcgccagc aggacagcgt
     1801 gaatcgaatt cacctccagc agcgcgcgcc gcggactccc tggccgacgc ccggtgtcgg
     1861 ccagccagtt gacttcctgg accggcggat ggtgctgacc gaacagcacc agccgatgcg
     1921 cgtcgagatc ctcagcccgc tgcggcacac cgtgcttttt caggtattca ggcgcggcca
     1981 cgacgttcca ttgcatcgtc agcaggtgcc gctgcacaag gtcaggctgt ttcggcggat
     2041 gcatgcggat cgccacatcg gcctcgcgca tcgccaggtc gagctccgta tcgtcgagca
     2101 gcagcgatat cgatacatcc ggataggctt ccaggaaccc ctgcagacgc ggcgccagcc
     2161 aggacgatcc gaacccgacg gtcgtggtga tcttcagccg accggccggc ttttccttgc
     2221 tctcggtcaa cagtgcctcg gtgatcgcca gcttggcgaa cacctcgcgg accgtacggt
     2281 tcagcgcttc gccctgttcg gtcaggatca gtccacgggc atgacgatgg
//
LOCUS       DASWQN010000206         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1473200, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000206 DASWQN010000000
VERSION     DASWQN010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473200"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="VGC82_03400"
     CDS             <1..1103
                     /locus_tag="VGC82_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HEY0422349.1"
                     /translation="EPTRQELLRRMNMSPGGTAALVAMRRDLLGHLRDNPSLKPLDYD
                     LRHLLGSWFNRGFLELRRIDWQTPAAVLEKLITYEAVHEIQGWEDLRRRLAADRRCFG
                     FFHPALPGEPLIFVEVALTEGLAASVQPLLAREGDEVAERARERAADTAIFYSISNCQ
                     DGLRGISFGNFLIKQVVEELKAELPGLVRFSTLSPVPGFRRWLERRLAAEDAANVLRE
                     DEMAELRLAAGQPIEEGDPAALLGGLLGTERWWETPQLADALRLPLTRLCATYLTTTV
                     ESRGSAEGKSARSRAPADPVARFHLGNGARLERVNWLGNTAARGIKESYGIMVNYLYD
                     PDEIEANHEGFVRGMVVRSAAVDALVGDAAEG"
     gene            complement(1286..1678)
                     /locus_tag="VGC82_03405"
     CDS             complement(1286..1678)
                     /locus_tag="VGC82_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422350.1"
                     /translation="MAIGTLENGAVFSVQIEGGQRHRTSLQIDITGTEGVLRVTNPRA
                     FQNKDDNAIEGVNGDGDTLALLPVPSEYRSLADSGLDVSQQDVAYLYRAYAQDRKNGT
                     LEASSFADAVRQHKIIDRMFQTSDAVFG"
     gene            1942..2412
                     /locus_tag="VGC82_03410"
     CDS             1942..2412
                     /locus_tag="VGC82_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422351.1"
                     /translation="MLDQVPQKARTRAETALQTDDVPNALGFRQGQQLFGFGGGGRER
                     PLAINVLARRDHDPGEPRMIESRGQDHDEINIRAINQRPLVTEGVRDAERARDRIRSC
                     LRPACDRNDLELLKRLQCGDVAVSCPAARPDDAHPDSFTAHCHILDQLPRAPGR"
     gene            2484..>3280
                     /locus_tag="VGC82_03415"
     CDS             2484..>3280
                     /locus_tag="VGC82_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0422352.1"
                     /translation="MDHLGTLSAFVQAAETGSFVTAGKNVGLTASAVGKGVARLEHRL
                     GIRLFHRSTRSMTLTEEGRLFLDRCRRIFEEIEAAEMELTKNAAAPSGRLRVSLPHVA
                     VLLMPVVAEFMEAYPAIVLDLDFTDRLVDVIEEGFDVVIRTGAAVDSGLMRRSLGRFR
                     GRLVASPAYLGRHGLPLVPADLAAHNCLRQRSPGSGKLNKWPLSGPAGHGRLAIPETM
                     SASTIDPLVHLAERGFGIAFLPPFAVAAQIKRGALVPLLEGHIRETG"
BASE COUNT          559 a         1025 c         1096 g          600 t
ORIGIN      
        1 ccgagccgac ccggcaagaa ctgctgcgcc ggatgaacat gtcccccggc ggcaccgcgg
       61 cgctggtcgc catgcgccgc gacctgctcg gacacctgcg cgacaacccc tcgctgaagc
      121 cgctagacta cgacctgcgg catctgctgg ggtcctggtt caaccgcggt ttcctggagc
      181 tgcgccgcat cgactggcag acccccgcgg cggtgctgga aaagctgatc acctatgagg
      241 cggtgcacga gatccagggg tgggaggacc tgcggcggcg gttggcggcc gaccggcgct
      301 gtttcggctt tttccatcct gcgctgccgg gcgagccgct gatcttcgtg gaggtggcac
      361 tgacggaggg cctggccgcc tcggtccagc cgctgcttgc gcgcgagggc gacgaggtgg
      421 cggagcgggc gcgcgagcgt gccgcggaca cggcgatctt ctactcgatc tccaactgcc
      481 aggacgggct gcgcggcatc tcgttcggca acttcctgat taagcaggtg gtcgaggagc
      541 taaaggccga gctgcccggc ttggtgcggt tctcgacgct gtcgccggtg ccgggtttcc
      601 ggcgttggct ggaacggcgg ctggcggccg aagacgctgc caacgtgctg cgcgaggacg
      661 agatggccga gctgcggctg gcggccgggc agccgattga agagggcgat cccgcggctt
      721 tgctgggcgg tttgcttgga accgagcgct ggtgggagac cccgcagctt gccgacgcgc
      781 tgcgcctgcc gctgacccgg ctttgcgcga cctacctgac gacaacggtc gaatcgaggg
      841 gttcggccga gggcaagagc gccaggagtc gggcgccggc tgacccggtg gcgcggttcc
      901 atttgggcaa cggcgcccgg ctggagcggg tgaactggct gggcaacacg gcggcgcgcg
      961 ggatcaagga gtcttacggg atcatggtga actatttgta tgatcccgat gagatcgagg
     1021 ccaatcacga ggggttcgtg cgcgggatgg tggtgcggtc ggcggcggtg gatgctttgg
     1081 tgggggatgc ggcagagggg tgatgcagcg ttggcgtcca ctggagttgc ggatcagcgc
     1141 ttgggtctaa cagttagcgc acggccgggt agcgtctcag tttgatcatc cgaatctggt
     1201 agtttccgaa ctgtctgctt ctgggcacaa aacccaggag acggacggag tttggcttta
     1261 gaccacgttt tggttctggc gccctctagc caaacacagc gtcggaggtt tggaacatcc
     1321 ggtcgatgat tttgtgctgc cgaacagcat ccgcgaagct ggacgcctcc aacgtcccat
     1381 tctttctgtc ctgcgcataa gcgcgataga gataggcaac gtcctgttgg ctgacatcca
     1441 gccccgagtc tgcgagggat cgatattcag agggcaccgg aagcagggcc agggtatctc
     1501 catcgccatt cacgccctcg atggcgttgt catccttatt ttgaaaggca cgcgggttgg
     1561 tgacccgcag aacgccctcg gtgccggtga tatcgatctg aaggctggtg cgatggcgtt
     1621 gcccgccttc gatctggacc gagaacacgg caccgttctc caatgtgccg atcgccatcg
     1681 ccgcatccgg cctgatggtc gggaactgct cgcctgtctc cgggaccgtg atgacgggaa
     1741 ctggttcctg acgatggcgg tcagctgctt cgggaacccg acggcatgga acagcacatc
     1801 gccgaagtgg cctccgtaaa cggaaagggt gttggcgaag ttggcaatat cgaagctcca
     1861 tgaatgaatt gcgggcattg tgtcgccgaa ggcgtcgacg ctgacggtca tgtgcactga
     1921 ccgcatgtcc ccgacatagc cttgctcgac caagtcccgc agaaagcgcg aactcgggcc
     1981 gaaacggcgc tgcaaaccga cgacgtgccg aacgcccttg gcttccgcca aggccagcag
     2041 ctcttcggat tcggtggtgg tggtcgagag cggccactcg ctataaacgt ccttgcccgc
     2101 cgcgatcacg atcctggcga gccgcgcatg atcgagagca ggggtcagga tcacgacgag
     2161 atcaacatcc gggcgatcaa tcagcgcccg ctcgtcaccg aaggcgtgcg ggatgccgaa
     2221 cgtgctcgcg accgcatccg cagttgcctg cgtccggctt gcgatcgcaa cgatctcgaa
     2281 ctcctcaagc gattgcagtg cggggatgtg gccgtatcgt gcccagccgc ccgtcccgac
     2341 gatgcccacc cggattcttt cactgctcat tgccacatcc tcgatcagct acctcgtgct
     2401 cccgggagat aggccgcatt tcagcgggaa aaacaggctg gcattccggt gaccccggaa
     2461 ccaaattccg ctatccgagg atgatggacc acctcggcac cctcagcgcc ttcgttcaag
     2521 ccgcggaaac cggcagtttc gttaccgcag gcaagaacgt gggcttgacg gcatccgctg
     2581 tcggcaaagg cgttgcccgg ctcgaacacc gcctcggcat ccgcctcttt caccgcagca
     2641 cccgcagcat gacgctgaca gaggagggac ggctgttcct tgaccggtgc cgccgcatct
     2701 tcgaggagat cgaggccgcg gaaatggaac tgacgaagaa cgcagccgcg ccgagcggcc
     2761 gccttcgcgt cagcctgcct cacgtggccg tgctcctgat gcccgtcgtt gccgaattca
     2821 tggaagccta tcccgccatc gtgctcgacc tcgacttcac cgaccggctg gttgacgtca
     2881 ttgaggaagg gttcgacgtg gtgatccgga ccggcgccgc cgtggacagc gggttgatgc
     2941 gtcgcagcct gggccgcttc cgtgggcggc tcgtcgcctc cccggcttac ctcgggcggc
     3001 acggtttgcc gctcgtgccc gcggaccttg cggcccataa ctgccttcgc cagcgctccc
     3061 ccggaagcgg gaagctcaac aaatggccgc tgtccggccc ggccgggcat ggacgccttg
     3121 ctattccgga aacaatgagc gcaagcacca tcgatcccct ggtgcatctg gccgaacggg
     3181 ggttcgggat agccttcttg cctccgttcg cggtggccgc ccagatcaag cgcggcgccc
     3241 tggtcccgct gctcgaaggg cacatccggg aaacgggtga
//
LOCUS       DASWQN010000207         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1473371, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000207 DASWQN010000000
VERSION     DASWQN010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473371"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /gene="pstC"
                     /locus_tag="VGC82_03420"
     CDS             complement(<1..511)
                     /gene="pstC"
                     /locus_tag="VGC82_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459629.1"
                     /note="part of the ATP-dependent phosphate uptake system
                     PstABCS responsible for inorganic phosphate (Pi) uptake
                     under Pi starvation conditions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HEY0422353.1"
                     /translation="MQQVATVAGRPVPGAPPSSMRARKRRMGDPVFRVVVAAAAFFVL
                     ISLGAAALSMFIGGLPAFRKFGLAFIYSSEWNPVRQEFGALVPIFGTLVTSALAMLIA
                     VPVSFGIAVFLTEVSPNWMRGPVASAIELLAGIPSIIYGMWGLFVFVPFMSDHVEPWL
                     IEHVGPLPVI"
     gene            complement(576..>1509)
                     /gene="pstS"
                     /locus_tag="VGC82_03425"
     CDS             complement(576..>1509)
                     /gene="pstS"
                     /locus_tag="VGC82_03425"
                     /inference="COORDINATES: protein motif:HMM:NF008171.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY0422354.1"
                     /translation="GAGSTFVYPILSAWSEAYNQAKGVRVNYQSIGSGGGIAQIKAAT
                     VDFGASDAPLAPDQLRTAGLTQFPLVIGGVVPVVNLQGIAPGQMKFTGPLLADLYLGK
                     IKKWNDPAVAAVNPGVTLPDQNVTIVHRSDGSGTTFNWVNYLSKVSPEWKDKVGEGTS
                     VSWPVGVGGKGNEGVAEYVNRIKGSIGYVEYAFVLQNKMTFGQVQNRAGQFITPDAAS
                     FQAAAASADWTKADSFYLVITDAPGQQAYPVAATVFILMYKQPKDAARSAAAIEFFRW
                     ALEHGDAQAAKLDYVPLPPALVTQVQASWKSEIK"
BASE COUNT          297 a          469 c          485 g          258 t
ORIGIN      
        1 cgattaccgg caacggacca acatgctcga taagccaggg ttccacgtgg tcggacatga
       61 acgggacgaa gacgaatagg ccccacatgc cgtagatgat gcttgggatg ccggcgagca
      121 gctcgatcgc ggaggccacc gggccgcgca tccagttggg gctgacttcg gtcaggaaca
      181 ccgcgatgcc gaagctgacc ggcacggcga tcagcatggc gagggcggag gtcaccagcg
      241 tgccgaagat cggcacgagg gcaccgaatt cctggcgcac cgggttccat tcggagctgt
      301 agatgaaggc gaggccgaac ttgcggaacg ccgggaggcc gccgatgaac atggaaagcg
      361 ccgcggcacc gaggctgatc agcacgaaga aggcggcagc cgccacgacc acgcgaaata
      421 ccggatcgcc catccgacgc ttgcgggcgc gcattgagct gggtggtgca ccaggaaccg
      481 gtcgtccggc cacggttgcg acttgctgca tcaagtaaag gcctcgtcag gatggaacac
      541 gacgaggcgg accagcacgg tccgcccctt gcggactact tgatctcgct tttccaggaa
      601 gcctggacct gcgtcacgag ggctggcggc agcggcacgt aatcgagctt cgccgcttgc
      661 gcgtcgccat gctccagcgc ccagcggaag aactcgatcg cggcggcaga ccgcgccgcg
      721 tccttgggct gcttatacat caagatgaaa acggtcgctg cgaccggata agcttgctgg
      781 cccggcgcgt cggtgatgac caggtagaaa ctgtcggcct tcgtccagtc ggcgctcgcc
      841 gcagccgctt ggaagcttgc ggcatccgga gtgatgaact ggccagcgcg gttctgcacc
      901 tggccaaagg tcatcttgtt ttgcagcacg aaggcgtatt cgacgtagcc gatcgaaccc
      961 ttgatgcggt tgacgtactc ggccacaccc tcgttgccct tgccgccgac gccgacgggc
     1021 caagacaccg atgtgccctc gccgactttg tccttccact ccgggctcac cttcgaaaga
     1081 tagttcaccc agttgaacgt cgtgccggac ccgtccgagc gatgtacgat ggtgacgttc
     1141 tggtccggca gggtcacacc aggattgacc gcggcaacgg ccgggtcgtt ccatttcttg
     1201 atcttgccca gatacaggtc agcaagcagc ggtccggtga acttcatctg gccgggtgcg
     1261 atgccctgca ggttaaccac tggcaccacg ccgccgatca ccaagggaaa ctgggtcagg
     1321 ccagcggtcc tgagctgatc cggcgccagt ggcgcgtccg atgcgccgaa gtccacggtc
     1381 gcagccttga tctgcgcaat gccgcctccg gaaccgatcg attggtagtt gacccgaacg
     1441 cctttggctt ggttataggc ctctgaccat gcggacaaga tgggatacac gaaggtggat
     1501 ccagcgccg
//
LOCUS       DASWQN010000208         3110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1869620, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000208 DASWQN010000000
VERSION     DASWQN010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869620"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VGC82_03430"
     CDS             complement(<1..224)
                     /locus_tag="VGC82_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422355.1"
                     /translation="MKIGAVMFFTTDSMQPAALGRALEERGFESLWVPEHTHVPSTRL
                     SAYPGGGALPRPYYDIYDPFLALNAAAAATT"
     gene            651..1118
                     /locus_tag="VGC82_03435"
     CDS             651..1118
                     /locus_tag="VGC82_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0422356.1"
                     /translation="MRPQVGDFLVRSDGEGKDNPIAAADLTIGDPPVLAWAVDPASKV
                     LRDGSGLNQVLLLRFDASALGPETKPHAADGVVAYSAVCTHAQCPVSGWISDRQVLHC
                     PCHQSEYDPRQNAKVVGGPAPRSLPALPLKVADGKLQVAGPFIGRVGMSQKVM"
     gene            1159..2886
                     /locus_tag="VGC82_03440"
     CDS             1159..2886
                     /locus_tag="VGC82_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HEY0422357.1"
                     /translation="MVGNSQIGGLALALALAALGGTTAAKAGEVSNYNEVTQQRLEHP
                     EAGNWLTYRRTYDGQGYSPLKQINADNVKSLIPVWTYSTGVNEGHEAPPMVNNGVMFV
                     STPYGQVLALNAKTGDLIWRYKHELPEDLFQLHPTNRGVAMWKDRLFMAATDDHLIAI
                     DAKTGKEIWNVKVQDYKAGQYMTLEPLVVNGKVMVGGSGGEYGVRGYVVAYDADSGKE
                     LWRTFTVPAPGEPGSDTWEGDAWKTGGGSVWLTGTYDPERHIAYWGTGNAAPWPGDQH
                     PGDNLYTSSTIALDPDTGKMRGFHQYHQNDSWDWDEMDPPILIKDMTYEGQKFDGLVH
                     PARDGYLWLLRQNADNIGFVYGKPFVGQNAFKGLDKAGRPIVDPEHKPTTGKPMTFCP
                     SLWGGKDWPSASYNPDTRLLYIPANNNMCETLEGKKQQLTVGQLWLGADADNAKLIPG
                     ADHIGELQAWDVSTGKQMWMHPFPSQLFGSTLTTGGDLVFVGGTNDRMFRAFDAKSGK
                     LLWEQKTNSGIMGMPVAYEVDGTEYIAIQSGWGVDAQRIQDMLAKTSLKLDPNVPQGG
                     VVWVFAVKK"
BASE COUNT          618 a          879 c         1074 g          539 t
ORIGIN      
        1 gtcgtggccg cggcggcggc attgagcgcc agaaacgggt cgtagatgtc ataatacggc
       61 cgcggcagcg caccgccgcc gggatacgcc gacagccggg tggacggcac atgggtgtgc
      121 tccggcaccc acaacgactc gaatccccgc tcctccagcg ctcggcccaa cgcggccggc
      181 tgcatcgaat cggtggtaaa gaacatgacg gcgccgattt tcattctggt tcccccgctt
      241 tcaaatccag tgtgagcaag ccgacgggct ggacgtcaag tggcgggcga ggccgtcacg
      301 aagtcaatgc gggcccgcag cggcaccggc atcaggttcc ggctgggaca gtacggaagc
      361 gggatggcaa cgatcgccgc cattcgcgca gcagcatttg gcacatcgtg cggaagggct
      421 ggtgccgaag gtccccctga actggcgcgc gaaccttcga cgatctaggc tcgctgaata
      481 acggcaacag gggagagagt gacagcgtga cagaactgac cccgattctt gaccaagacc
      541 agtgctcggc ggccctgtta cgccgggatt tgttgcgcgt cggtcccgca gcgacgcttg
      601 tagcaatatc gtcgcaggtt cgtgccgaga gtgatgaccc ggcgagcaaa atgcgcccgc
      661 aggtcggcga ctttctggtt cgtagcgacg gtgagggaaa ggataacccg atcgccgctg
      721 ccgatttgac gatcggcgat cctccggtgc tggcctgggc ggtagatccg gcgagcaagg
      781 tgctgcgcga tgggtccggg cttaatcagg tgttgctgct gcggttcgat gcgtcggcgc
      841 tggggccgga gaccaagccg cacgcggcgg acggggttgt cgcctattcg gcggtgtgca
      901 cgcatgcgca atgtccggtt tccggttgga tcagcgaccg gcaggtgctt cactgtcctt
      961 gccatcagtc ggaatatgat ccgcggcaga atgcgaaggt ggtgggaggc ccggcaccac
     1021 ggtcgttgcc ggcattgccg ctgaaggtag ccgatgggaa acttcaagtc gccggcccgt
     1081 tcatcgggcg agtggggatg agtcagaagg tgatgtgagg ccgggctgag ggacggaacg
     1141 ttgaaaaagg agaagaaaat ggtcggaaac agtcaaatcg gtggcttggc gcttgccctg
     1201 gcgctggcgg cactcggggg aaccactgct gcgaaggcgg gcgaggtatc caactacaat
     1261 gaggtgacgc agcagcggct ggaacatccg gaggccggca actggctgac gtatcgacgc
     1321 acgtatgatg ggcagggcta cagcccgctg aagcagatca acgccgacaa cgtcaaatcg
     1381 ctgatcccgg tctggactta ttccaccggg gtgaatgagg gtcatgaggc cccgccgatg
     1441 gtgaacaacg gcgtgatgtt cgtctctaca ccgtatggcc aagtgctggc gctgaatgcg
     1501 aagaccggcg acctgatctg gcgctacaag cacgaactgc cggaagatct gttccaactg
     1561 catccgacca accgcggtgt ggcgatgtgg aaagaccggc tgtttatggc tgcgaccgat
     1621 gaccacttga tcgcgatcga cgccaaaaca ggcaaggaaa tctggaacgt caaggtgcag
     1681 gactacaagg cgggccagta catgacgctg gaaccgctgg tggtgaacgg caaggtgatg
     1741 gtcggcggat cgggcggcga atatggcgtc cgcggctatg tcgtggccta tgacgcggat
     1801 agcggcaagg aactgtggcg cacctttacc gtgccggccc cgggcgagcc gggttccgac
     1861 acctgggagg gcgacgcctg gaaaaccggc ggcggctcgg tatggctgac gggaacctat
     1921 gatccggagc ggcatattgc ctactgggga accggcaacg cggcgccgtg gcccggtgat
     1981 cagcatccgg gcgataacct ttacaccagt tctaccatcg cgctggatcc cgataccggc
     2041 aaaatgcgcg gtttccatca gtaccatcag aacgattcct gggactggga cgagatggat
     2101 ccgccgatcc tgatcaagga catgacctat gaaggccaga agttcgacgg cctggtgcat
     2161 ccggcgcgcg acggctatct gtggctgctg aggcagaatg ccgacaacat cggtttcgtc
     2221 tatggcaagc cgtttgtcgg gcagaacgca ttcaaggggc tcgacaaggc cgggcgcccg
     2281 atcgtcgatc cggagcacaa gccgaccacc ggcaagccga tgacgttctg tccgtcgctg
     2341 tggggtggca aggactggcc gtcggcgtcg tacaatcccg acacccggct gctgtatatc
     2401 ccggccaaca acaacatgtg cgaaacgttg gagggcaaga agcagcaact gacggtgggc
     2461 caactctggc ttggcgctga tgccgacaac gcgaagctga tccccggcgc ggaccatatc
     2521 ggggagctac aggcgtggga cgtttccacc ggcaagcaaa tgtggatgca tccgttcccg
     2581 tcgcagttgt ttggctcgac gctgacgacc ggcggcgatc tggtgtttgt cggcggcacc
     2641 aacgaccgga tgttccgggc gttcgatgcc aagtcgggca agctgctgtg ggagcagaag
     2701 accaactccg gcatcatggg catgccggtg gcctatgagg tcgatggcac cgagtacatc
     2761 gcgatccagt cgggctgggg cgtggatgcg cagcgtatcc aggacatgct ggcgaagacc
     2821 agcctgaagc tggatccgaa tgtgccgcaa ggcggtgtcg tgtgggtctt cgcggtcaag
     2881 aagtagcttg tcgggcggcg tggcctcccg cagcgcggcc tcacgcgatg gggctggcca
     2941 cgtcgcatga ttaggtcaat ggcgtgggca ggcgacggcg ccgtcgctat gtcgattggc
     3001 cggaaacggt ggtgtcgatc cgccaccaga tcggcggaac ttgccggttg cagcggacgc
     3061 gacccagccg gcgcgttccg cacggtttct gactccagaa gcggtcgaag
//
LOCUS       DASWQN010000209         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_154194, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000209 DASWQN010000000
VERSION     DASWQN010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154194"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VGC82_03445"
     CDS             <1..586
                     /locus_tag="VGC82_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422358.1"
                     /translation="TGAPAITALEHKFLEAACIAQKATGAPIITHTQDGQAGPEQQQA
                     FAKGGVAAHRCLIGHCCGNADPTYHQRVVDGGSYIGFDRIGLLRFQPDEIRADNLVRL
                     VESGHQAQVIMSQDRHCGWLGKFARQIGPEEQARIDALTKQGKWPPPYTYLFTDFVPM
                     LKSRGLSDQQIDSVLEDNPRRFFAGEILPLLNRG"
     gene            590..2191
                     /locus_tag="VGC82_03450"
     CDS             590..2191
                     /locus_tag="VGC82_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0422359.1"
                     /translation="MEADYVVVGAGSAGCVVAARLSETGAKVILLEAGPKDSLLWIHI
                     PAGILKLLYHPVVNWNYSAEGTEGSGGRRIHWPRGKTLGGSSSINGMLYVRGNPADFD
                     GWAQMGCRGWSFDDVLPYFRKSEDYKQGADAEYRGNGGPLKVEDYRTILPLTHRFIEG
                     AQQAGHPFSKDLNGRQQEGVGYSQMTRKGRWRGSTAQTFLKEARGRPNLRVETEALAT
                     KLLFEGTRCVGVTFRQRGQTVEARAHKEVILCGGAVNSPHLLQVSGVGPAEHLQSIGV
                     PVVHDLRGVGSNLQDHYVARVSHRVKGSVSINTLARGIRLVREAMRFAFEGRGALTFG
                     VTTAQVFCRSAPEKASPDLQLLFTPASYDAAKFGELEREDGMTVAVCPVRPDSRGTIM
                     ARSADPAEKPVITPNYLSDPADIRVLTAGVRFARAIFATPAIAQHSVVETLPGADVNT
                     DDGLLDYARRYGTTIYHPVGTCRMGDGPSAVVDSRLRVHGLQALRVIDASVMPTLTTG
                     NTNAPTIMVGEKGAAMIKEDHAAAG"
     gene            2301..2666
                     /locus_tag="VGC82_03455"
     CDS             2301..2666
                     /locus_tag="VGC82_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422360.1"
                     /translation="MIRFARAEAGSTVIDVYPGDGDWTRLFSDIVGPEGRVYSFVPAE
                     LAHFKNDPVGRMRTLAKEPGRENVEAVSADLVAMPEATQPTDVLWLHLFYHDLHTALI
                     QKKGSFRPAADDPDCEEGW"
BASE COUNT          522 a          800 c          877 g          494 t
ORIGIN      
        1 gaccggcgcg ccggcgatca ccgcgttgga gcacaagttc ctggaggcag cctgcatcgc
       61 gcagaaggcg accggcgctc cgatcatcac tcatacgcag gacggtcagg caggtcccga
      121 acagcaacag gctttcgcca agggcggggt cgcggcccat cggtgcctga tcgggcactg
      181 ctgcggcaac gccgacccaa cataccacca gcgcgtcgtg gatggcgggt cctatatcgg
      241 cttcgaccgc atcgggctgt tgcggttcca gccggatgaa atccgagcgg acaatctggt
      301 tcgactggtt gaaagcggtc atcaggcgca ggtcatcatg agccaggatc gccactgcgg
      361 ctggttgggc aagttcgccc gtcagatcgg ccccgaagag caggcacgaa tagatgcctt
      421 gaccaagcaa ggcaaatggc cgccacctta cacttattta tttactgact ttgttcccat
      481 gttgaagtcc cgaggcttgt ccgatcagca gatcgactcg gtactcgagg ataatccgag
      541 acgtttcttc gcaggcgaga tcttgccgtt gctgaaccga ggctgaccga tggaagccga
      601 ttatgtcgtc gtcggtgccg gttcggctgg ttgcgtcgtc gccgctcgac tgagtgagac
      661 cggcgccaag gttatactgc tggaggccgg gccgaaggac tccctgctgt ggatccacat
      721 accggcgggc attctgaagc tgttgtacca tccggtggtc aactggaact acagcgccga
      781 gggcaccgaa ggtagcggag ggcggcgcat ccactggcct cggggcaaga cattgggcgg
      841 gtcgagttcg atcaacggga tgctgtatgt aagaggcaat ccggcggatt tcgatggctg
      901 ggcacagatg ggctgccgcg gctggagttt cgacgacgtc ctgccgtatt tccgcaagtc
      961 ggaggattac aagcaaggcg cggatgccga ataccgcggc aacggcggtc cgctaaaggt
     1021 cgaggactac cggacgatcc tgccgctgac ccacaggttc atcgaaggcg cgcagcaggc
     1081 cggacacccg ttcagcaaag acctgaacgg gcgccagcaa gagggcgtcg gctactcgca
     1141 gatgactcgc aaagggcgat ggcgcggatc gaccgcacag actttcctga aggaagcccg
     1201 gggacgcccg aacctgcggg tcgaaaccga ggcgctggct acgaagttgt tgttcgaagg
     1261 tacgcgctgt gtcggcgtca cgtttcgcca gcgtgggcaa acggtcgagg ccagggcgca
     1321 caaggaagtc atcctgtgcg gcggtgctgt caattcgccg catctactgc aggtatcggg
     1381 cgttgggccg gccgaacact tgcaatccat tggcgttccg gtggtgcacg acttgcgagg
     1441 cgttggatcg aacctgcagg atcattacgt cgccagggtt tcgcaccgtg tcaaaggatc
     1501 ggtgtcgatc aacaccctcg cccgaggcat ccggctggtt cgcgaagcga tgcgtttcgc
     1561 tttcgaaggt cgtggcgcgc tgacgttcgg tgtgacgacg gcacaggtct tttgccggtc
     1621 agcacctgaa aaggcgagcc ctgacctgca gcttctgttc acgccggcca gctacgacgc
     1681 tgctaaattc ggtgaactgg aacgcgaaga tggaatgacc gtggcggtct gtccggtgcg
     1741 cccggacagt cgcggcacaa tcatggcccg aagcgcggac cccgcagaga agccggtcat
     1801 cacgccgaac tatctatccg atccggccga tatccgcgta ctgaccgcgg gggtgcgctt
     1861 cgcccgggcc atcttcgcga caccagcaat cgcgcagcac agcgtggtgg aaacgctgcc
     1921 gggtgccgac gtcaacaccg acgacggctt gctggactac gcgaggcgct atggcaccac
     1981 gatctaccac cccgttggca cctgccggat gggtgacggt ccgtcggccg tcgtggactc
     2041 gcgcctgcgt gtgcacggcc ttcaagcgtt gcgggtcatc gatgcgtccg taatgccgac
     2101 cttgacaacg ggcaacacca atgcgccgac gattatggtc ggtgagaaag gtgccgcgat
     2161 gatcaaggaa gatcacgccg cggcgggtta aagcgaactt cggctgactt gaaagtgggc
     2221 gccatgacgg gagctggctt tccgttcggc tatcgcgggt ttgtcagagg gccgtgattc
     2281 tcaacacgga gctatccgag ttgatccggt tcgcacgagc cgaggcgggc tccaccgtca
     2341 tcgacgttta cccaggcgac ggcgactgga cccgtctctt ctccgacatc gtgggacccg
     2401 aaggaagggt ctacagcttt gtgccggccg aactcgcgca cttcaagaac gacccggtcg
     2461 gccgcatgcg gacgcttgcg aaggagccgg gccgagagaa cgtcgaagcc gtctcggccg
     2521 acctcgtggc gatgccggag gccacgcaac caaccgatgt cctgtggctg cacctgtttt
     2581 accacgatct ccacaccgcc ctaatccaga agaaggggtc attccgtccg gctgctgacg
     2641 atccggattg cgaggagggt tggtgagtac cgactgttct gcgtccatgg ttc
//
LOCUS       DASWQN010000210         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1341814, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000210 DASWQN010000000
VERSION     DASWQN010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341814"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VGC82_03460"
     CDS             <1..511
                     /locus_tag="VGC82_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0422361.1"
                     /translation="RIDVVQYGWNMFDRRMQHEIFPWCIVNGVGVMAYGSLAYGMLTG
                     SFQAGMAFDEADWRSKRGALGGLNLFRTMFGPDHFERNLRAVEELKPLAAKYGKTLPQ
                     FALRWTLSHPAISTGLVGFRNPNEVAENLGALGFDISQQDMAAVNAIFARNGAVTEPP
                     GWLEDDSPA"
     gene            543..>1901
                     /locus_tag="VGC82_03465"
     CDS             543..>1901
                     /locus_tag="VGC82_03465"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0422362.1"
                     /translation="MPVQRFAPMSKDFATFDCDAHVTEPPLIWERAAEYLTREELAAL
                     RTTIWWDEESRQLIVIGRAGVGIGSQRRGGIPGTMRVISNAGPGVKHDIQRALNVRNL
                     KAGTALSQEQVSYIDFGGSYEPEPRLRDMDMQGIDQVMIIPTDIDTYPWLLDAVGAYA
                     FCKAYNQWAYDYTKTDPDRLFFAALLPMQNAQFAVQEAYRVAAQGCRVALVRPTDAMG
                     NYPLQPKYEPVWNALEETGMVYGMHPFPAGGALKPPGYAEQYSGAELIRRTISASGVP
                     HSFLTNVQNFQSEAALWVTTALMCGFFERHPNLKAAVFEASSTWLSFLLDECDKNYRL
                     YRNERSMPPLKRLPSETFFAHCMTGFEGDEAPPPRFPDFYGDILIWSSDVYHHDGDDA
                     WRAMETMQKYHLPTENQSKFLGANARRLYRIDTPKKIVRDRVTEIERPNWWPTEEEVR
                     AAL"
BASE COUNT          373 a          602 c          587 g          339 t
ORIGIN      
        1 acgtatcgat gtggtgcagt acggctggaa catgttcgac cgccgcatgc agcatgagat
       61 cttcccgtgg tgcatcgtca atggtgttgg tgtcatggcc tacggttccc tggcctacgg
      121 gatgctgaca ggatcgtttc aggccggcat ggcgttcgat gaagcggact ggcgctcgaa
      181 acgcggggcg ctcggcgggc tgaacctgtt ccgcaccatg tttggaccgg atcatttcga
      241 gcgcaacctg cgtgccgttg aggaactgaa gccactcgcc gcgaaatacg gcaagacgct
      301 gccgcagttc gcgctgcgct ggacgctgtc acatcccgcg atcagcacgg gtctagtggg
      361 gttccgcaac ccgaacgaag ttgccgaaaa tctgggcgcg ctcggattcg atatttcgca
      421 gcaggacatg gcggcggtca acgcgatctt cgcccgcaac ggtgccgtca ccgaaccgcc
      481 gggatggctg gaggacgatt cgcccgcttg agggaaacgc aaccacgaca gggaaaacat
      541 aaatgccggt tcagcgcttt gcgccgatgt cgaaggattt cgccaccttc gactgcgatg
      601 cccatgttac cgagccgccg ctgatctggg agcgcgccgc cgaatacctc acgcgtgagg
      661 aactcgcagc gctgcgcacc acgatctggt gggatgagga aagccggcaa ctgatcgtca
      721 tcggccgcgc cggcgtcggg attggttcgc agcggcgtgg cggcattccc ggcaccatgc
      781 gggtgatctc caacgctgga ccgggcgtga agcacgacat ccagcgcgcg ctgaacgtcc
      841 gcaacctgaa ggctggcacc gcgctgtcgc aggagcaggt cagctacatc gactttggcg
      901 gctcctatga gccagagcca cggctgcggg acatggacat gcagggaatc gatcaggtga
      961 tgatcatccc gaccgacatc gacacctatc cgtggctgct cgacgctgtc ggcgcctacg
     1021 cgttctgcaa ggcatacaac cagtgggcct atgactacac caagaccgac cccgaccggc
     1081 tgttcttcgc cgccctgctg ccgatgcaga atgcgcagtt cgccgtgcag gaagcctatc
     1141 gcgtcgccgc ccaaggttgc cgggtcgcgc tggtgcgccc gaccgatgcg atgggcaact
     1201 acccgctgca gccgaaatat gagccggtgt ggaatgcgct cgaagaaacc ggcatggtct
     1261 acggcatgca cccgtttcct gccggcggcg cgctaaagcc acccggctat gcggagcaat
     1321 actccggtgc cgaactgatc cgccgcacca tctcggcctc cggcgtcccg cactccttcc
     1381 tgaccaacgt gcagaacttc cagtccgagg ctgcactttg ggtcaccacg gcgctgatgt
     1441 gcggtttttt cgaacgccat cccaacctga aggcggcggt gttcgaggcg tcctccactt
     1501 ggctcagttt cctgctggat gagtgcgaca agaactaccg gctttatcgc aacgaacgca
     1561 gtatgccccc gctaaagcgc ctaccgagcg aaacgttttt cgcgcattgc atgactggat
     1621 ttgaaggcga cgaggcgccg ccgccgcgct tcccggattt ctacggcgac atcctaatct
     1681 ggtcgtcgga cgtctaccac cacgacggcg acgacgcctg gcgcgcgatg gaaacgatgc
     1741 aaaaatatca cctgccgacg gaaaaccagt cgaagttcct cggcgccaat gcgcgccgcc
     1801 tgtatcgcat cgacacgccg aagaagatcg tccgcgaccg cgtcaccgag atcgaacggc
     1861 cgaactggtg gccgaccgag gaagaggtga gggccgcgct g
//
LOCUS       DASWQN010000211         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_286302, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000211 DASWQN010000000
VERSION     DASWQN010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286302"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VGC82_03470"
     CDS             <1..1068
                     /locus_tag="VGC82_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0422363.1"
                     /translation="YRSMVLATMAICIISFMVWLHHFFTMGAGPDVNAIFGIASMIIA
                     VPTGVKIYNWLFTMYGGRIRFSTPMMWSVGFMVTFIVGGLTGVLLAVPPADFVLHNSM
                     FLVAHFHNVIIGGVLFGAFAGLEYWFPKAFGFRLDERWGKIGFWFTFVGFYVTFVPLY
                     IAGMLGMTRRMQHYDVAAWHPWMLIAAFGMLLLSIGVVSQIVQLVVSIRHREALRDRT
                     GDPWDGRSLEWATSSPPPVFNFAFYPDVRGEDAYWALKQHARRHQLERGEPEYHDVEM
                     PRNSPTGFVCAFFATVMGFALIWHIWWMVILGGIGAFATFVVFAWRDHDEYVIPAAEV
                     ARIDRSNIEERRNLVSMAGAL"
     gene            1068..1709
                     /locus_tag="VGC82_03475"
     CDS             1068..1709
                     /locus_tag="VGC82_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome (ubi)quinol oxidase subunit III"
                     /protein_id="HEY0422364.1"
                     /translation="MAMTATITHGHADPHHLGRAVEHPGPPPKRIVTAYGFWIFLLSD
                     IVMFSCFFAAYAVLVNQTANGPKGSQIFDWTNLAIETVCLLLSSFSCGMASIAADLRN
                     RLWFYLSMAVTCVLGLAFLTIEFREFADLVSRGFGPSRSAFLTAFFSLVGCHGLHVSA
                     GVLWLLTMMAQVFAKGFRADIMRRMTCFALFWHALDIIWVAVFSIVYLLGGAP"
     gene            1706..>1986
                     /locus_tag="VGC82_03480"
     CDS             1706..>1986
                     /locus_tag="VGC82_03480"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEY0422365.1"
                     /translation="MSEPSEQVVEPLDVAPGDEGEHNLRTRLLGYVIGLGLALLLTAT
                     SFFVAGTHMVWQPSIPVAIIVLAIAQMGVHLVFFLHITTGPDNTNNVLAL"
BASE COUNT          308 a          621 c          584 g          473 t
ORIGIN      
        1 taccgctcca tggtgctggc cacgatggcg atttgcatca tctccttcat ggtgtggctg
       61 catcacttct tcaccatggg ggccggaccc gacgtcaacg cgatcttcgg cattgccagc
      121 atgatcatcg ctgtgcccac cggagtgaag atctacaact ggctattcac aatgtatggc
      181 ggccgaatcc gcttcagcac gcccatgatg tggtcggttg gcttcatggt gaccttcatc
      241 gtcggcggcc tgaccggcgt cctgctcgcg gtaccgccgg cggacttcgt tttgcacaac
      301 agcatgttcc tggtcgcgca ctttcacaac gtcatcatcg gtggggtgct gttcggcgcc
      361 ttcgcggggc tggaatactg gttcccaaag gcttttggct tccggctcga tgagcggtgg
      421 ggaaaaatcg ggttctggtt caccttcgtc ggcttctacg tcaccttcgt cccgctttat
      481 atcgcgggca tgctcggcat gacccgtcgc atgcagcact acgacgtcgc ggcctggcat
      541 ccctggatgc tcatcgccgc cttcggcatg cttttgctgt cgatcggcgt cgtcagccaa
      601 atcgtgcagc tcgtggtcag catccgtcac cgcgaggcgt tacgcgaccg caccggcgat
      661 ccctgggacg gccgttcgct ggaatgggcg acgtcgtcgc cgccgccggt gttcaatttc
      721 gccttttatc cggatgtgcg cggcgaggac gcctactggg cactgaagca gcacgccagg
      781 cgccatcaac tcgagcgtgg tgagcccgaa taccacgacg tcgagatgcc ccggaattcg
      841 ccgaccggat tcgtctgcgc cttctttgcc accgtgatgg gttttgctct gatctggcat
      901 atctggtgga tggtgatttt gggagggatc ggtgcgttcg ccaccttcgt cgtgttcgcc
      961 tggcgcgacc atgacgaata cgtgattccg gcggcagagg tcgcgcgcat agatcgctcc
     1021 aatatcgagg agcgccgaaa tctcgtcagc atggcgggag cgctctgatg gcaatgaccg
     1081 cgaccatcac ccacggccac gcggaccccc atcatctcgg gcgcgccgtc gagcatccgg
     1141 gcccgccgcc gaaacgcatt gtgaccgcct acggtttctg gattttcctg ctttccgaca
     1201 tcgtgatgtt ctcctgcttc ttcgccgcct atgcggtgct ggtcaatcag accgctaacg
     1261 gcccgaaggg ttcgcagata ttcgattgga cgaatttggc gatcgagacg gtctgcctgc
     1321 tgctctcgag cttctcctgc ggcatggcaa gcatcgcagc cgatcttcgc aacaggctgt
     1381 ggttttacct gtcgatggcg gtcacctgcg tgctggggct ggcattcctc actatcgaat
     1441 tccgcgaatt cgccgatctg gtctcgcgcg ggtttggtcc ttcacgcagc gcgtttttga
     1501 ccgcattctt ttcgctggtc ggatgtcacg gcctgcacgt ctcggcgggg gttctgtggt
     1561 tgctcaccat gatggcccag gttttcgcca agggctttcg ggccgacatc atgcgccgca
     1621 tgacgtgctt tgcgctgttc tggcatgcgc tcgacatcat ctgggtcgcg gtgttttcca
     1681 tcgtctattt gctcggaggt gcgccatgag cgagccgtcc gaacaggtcg tcgagccgct
     1741 cgatgttgcg ccgggcgatg aaggcgaaca caacctccgc accagattgc tcggatatgt
     1801 catcggcctc ggcctggcgc tgttgctgac cgcaacctcg ttcttcgtcg ctggaaccca
     1861 catggtctgg cagcccagca ttcctgtcgc catcatcgtg ctcgccatcg cgcagatggg
     1921 cgtgcatctc gtgttcttcc tgcatatcac aaccggtccc gacaacacca acaacgtgct
     1981 ggcgct
//
LOCUS       DASWQN010000212         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1473803, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000212 DASWQN010000000
VERSION     DASWQN010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473803"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VGC82_03485"
     CDS             complement(<1..350)
                     /locus_tag="VGC82_03485"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY0422366.1"
                     /translation="MKSKLLAAALLGTTLLSGVAYAQTATTDRSNANTAVHKDGQWRA
                     SKLIGVNVYNDNNDKIGEVEELIVDKSSKVENVVLGVGGFLGMGEHYVAVPMDKLKWV
                     DQPVRTSSTSTAPG"
     gene            complement(508..1362)
                     /locus_tag="VGC82_03490"
     CDS             complement(508..1362)
                     /locus_tag="VGC82_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422367.1"
                     /translation="MEQAMRPVGVADEVLEPAVAGVSWAAVAAGAVASLALTLVLLSL
                     GAGMGFAVVSPWAHSGVSATTFEIGTGLYFIVMAMISSAIGGYLAGRLRNRWIGVQSP
                     EVLFRDTAHGFLAWALASVVGAILLASPATTLLSGATAGAVQAASSPQASGPMDPYVD
                     MLLRPANPGPDSAANANSGEVRRELARLLTADFRNGGEPSAADRTYLTKVVAARTGLN
                     QADADKRVSDVINQVKSDLEKARKAAEHLAIWLTLSLFIGAFAATLAATEGGGLRDGT
                     WGRTSLRR"
BASE COUNT          312 a          487 c          493 g          316 t
ORIGIN      
        1 tcgcccggcg cggtcgatgt cgaggaagta cgaacgggtt ggtcgaccca tttcagcttg
       61 tccatcggaa ccgcgacgta atgttcgccc atgccaagga agccgccaac gccgagcacg
      121 acgttttcga ccttggagga cttgtcgacg atcagctcct cgacctctcc gatcttgtcg
      181 ttgttgtcgt tatagacatt gacgccgatc agtttcgaag cccgccactg cccatccttg
      241 tggacggccg tattggcatt ggaccggtcg gtggtggcgg tctgggcgta ggccaccccg
      301 ctcaacaaag tggtgccgag cagcgccgcg gcaagaagct ttgatttcat ttcagtctcc
      361 agtcagaaag tggaaggtga ttgttgttgg ccgtctacgc accgatcaat ggtgcaacag
      421 catgatgagg atgatcagcg ggatcggtat tcccaacagc cacagcagaa taggcatggt
      481 tctctccttt ggtttggttg cgagtggtta acggcgcagc gatgtcctgc cccaagttcc
      541 gtcacggagg ccgccgcctt cggtcgccgc cagcgttgcc gcgaaggcac cgatgaacag
      601 cgagagcgtc agccagatcg caaggtgttc tgctgccttg cgtgcctttt caagatccga
      661 cttgacctga ttgatcacgt cgctgacgcg tttatcggcg tccgcctggt tcaggccggt
      721 ccgggccgcg accaccttcg tcagataagt gcgatctgct gccgacggtt cgccaccgtt
      781 gcgaaagtcg gcggttaaca ggcgcgccaa ttcgcggcgt acttcaccgc tattggcgtt
      841 tgcggcgcta tccggtcccg gattggcggg acgcagcagc atgtcgacgt aggggtccat
      901 gggccctgag gcctgcggag aactggccgc ctgcacggcg ccggcggttg ctccgctcag
      961 caaggttgtc gcgggagagg ctagcaagat cgcgccgacc accgaagcca gtgcccaggc
     1021 caggaaccca tgcgcggtgt cgcgaaacag gacttcggga gattgaacgc caatccaccg
     1081 gttgcgcagc cggcctgcga ggtagccgcc gatcgccgac gaaatcatgg ccatgacgat
     1141 gaagtaaaga ccggtgccga tctcgaacgt tgtggcggaa acgccggaat gggcccaagg
     1201 cgacaccacg gcaaaaccca tgcctgcgcc gagcgacaac aagaccagcg tcaacgcaag
     1261 ggaggcgacg gcgcctgccg cgactgcggc ccatgaaaca cccgctactg ctggttccaa
     1321 aacctcgtca gcgaccccga cggggcgcat tgcttgttcc atggtgttct cccggcaact
     1381 tgatttcgat aacgagcaaa tgttggaact tgctgaaagt tccatccagg aacccgtttc
     1441 ctcgcgggga accgctctca tgttttgacg ttcttctccc gctgaatcat aaatggagga
     1501 cttcatgaac accaagatcc ttgccggcct gacgatggcc gcggcgctct cgttgccctt
     1561 ggcagcgaat gcgcaaggcg gcgccgctgc gggcgcgacc acgggtgc
//
LOCUS       DASWQN010000213         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2133855, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000213 DASWQN010000000
VERSION     DASWQN010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2133855"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VGC82_03495"
     CDS             complement(<1..247)
                     /locus_tag="VGC82_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY0422368.1"
                     /translation="MTTTLPIDQMIDTDRGTISREIFVSPEFHRQELENLFTRAWLFV
                     GHESQIPNPGDFFVSRMGQESVILCRDAQGAVHVFLNS"
     gene            complement(277..738)
                     /locus_tag="VGC82_03500"
     CDS             complement(277..738)
                     /locus_tag="VGC82_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY0422369.1"
                     /translation="MAHIKVQTEDFDVGAEIAALTAGRTDIGGIGCFVGTVRADAKTT
                     RSAVTGMTLEHYPGMTERAIERIADQAAERWPLLGCTVIHRVGALRPGENIVLVIAAS
                     SHRQAALDATAFLIDWLKTKAPFWKREDFAGGDGAWVDAREEDDAAASRWS"
     gene            complement(889..>2079)
                     /locus_tag="VGC82_03505"
     CDS             complement(889..>2079)
                     /locus_tag="VGC82_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422370.1"
                     /translation="FLAGLAAACLPRHVRAAEETPGVTATELKIGNTAAYSGPASAYG
                     IIARTEAAVFKMINDQGGVGGRKIVYDSLDDGYSPPKTVDQVRRLVEEDQVAFMFATI
                     GTPSNTAIYRYTNGRGVPLLFLGSGANKWGDPKNASWVMGWQPSYRTEAQIYTRYVLR
                     EKPDAKIAILYQNDDFGKDYPAGVRDVLGKDFESRVVKVVSYEVTDATIDSQAVALQA
                     SGADVLITAATPKFAAQTIRKIAELGWKPLHFMTNVSISVGSVITPAGPENARGMISA
                     AYLKDPTDPTWKDDPGMNQWRAFMAKYMPDADLTDGGPVFGYGVTMGLWQVLKQCGTD
                     FSRANIMRQAANLHDVEIPVLLPGIKVNTSPTNFHPIRQMQLTRWTGQHFELFGKVLE
                     GEAV"
BASE COUNT          358 a          646 c          651 g          424 t
ORIGIN      
        1 aggagttcag gaagacatgc accgcaccct gggcatcacg gcagaggatg acggattcct
       61 ggcccatgcg agagacgaag aaatcgccgg gattggggat ctggctttcg tgcccgacga
      121 acagccacgc ccgggtgaac aggttttcca gctcctgacg atggaattcg ggggaaacga
      181 agatttcgcg gctgatcgtg ccccgatcgg tgtcgatcat ctggtcgatg ggcagtgtcg
      241 ttgtcatgtc tgccattcct cgtgttgttg cacgcgtcag ctccagcggg acgccgcggc
      301 gtcgtcctcc tcacgggcat ccacccaggc gccgtcgccg ccggcgaagt cttcgcgttt
      361 ccagaacgga gccttggttt tcagccagtc tatcaggaag gccgttgcgt ccaatgccgc
      421 ctgacgatgc gacgatgcgg cgatgacgag aacgatgttc tcgcccggcc gcagcgcgcc
      481 gacccggtgt atcaccgtgc agcccagcaa cggccatcgc tctgccgcct gatcggcgat
      541 cctttcgatg gctcgttcgg tcatccccgg atagtgctcc agggtcatgc cggtgaccgc
      601 cgagcgagtg gttttagcgt cggcgcgtac ggtcccgaca aagcagccga tgccgccgat
      661 gtcggtgcgc ccggccgtca gcgcggcgat ttccgcgccg acatcgaaat cctcggtctg
      721 aaccttgata tgcgccatca ggatggaaac tagaccgttg cggccgattt gtcatcggct
      781 tggcggctag agcaatatcc gttctgacag acgcacggct tccgcacagg cccttgtttt
      841 cacgagcagc tttatccggt aggtgatccc acttggtcca atgatgctct aaacggcctc
      901 gccttccagc actttgccga acagctcgaa atgctggccg gtccagcgcg tcagttgcat
      961 ctggcggatc ggatggaagt tggtggggct ggtgttcacc ttgatgccgg gcagcagcac
     1021 ggggatctcc acatcatgca ggttcgccgc ctgcctcatg atgttggcgc gcgagaagtc
     1081 cgtgccgcac tgcttcagca cctgccacaa tcccattgtc acgccgtaac cgaacaccgg
     1141 gccaccatcg gtcaggtcgg cgtcaggcat gtatttggcc atgaaggcgc gccactggtt
     1201 catgcctgga tcgtctttcc aggtcgggtc tgtcggatcc ttcagatacg ccgcgctgat
     1261 catgccacgc gcattctctg gcccggccgg ggtgatcacc gaacccaccg agatcgagac
     1321 gttggtcatg aaatgcagcg gcttccagcc gagttcggcg atcttgcgga tggtctgcgc
     1381 cgcgaacttc ggcgttgccg ctgtgatcag cacgtcggcg ccggatgcct gcagcgccac
     1441 cgcctgcgaa tcgatcgtgg cgtcggtcac ctcgtaggag acaaccttga caacgcggct
     1501 ttcgaaatcc ttgcccagca cgtcgcgcac gccggcggga taatccttcc cgaaatcgtc
     1561 gttctgatac aggatcgcga tcttcgcgtc cggcttttcc cgcaggacat agcgcgtgta
     1621 gatctgcgct tcggtccggt agctgggctg ccagcccatg acccaggatg cattcttcgg
     1681 atcaccccac ttgttggcgc cgctgccgag gaacagcagc ggcacgcctc ggccgttggt
     1741 atagcgatag atggcggtgt tggacggagt gccgatggtg gcgaacatga acgcgacttg
     1801 atcttcctcg accaagcggc gcacctgatc gacagtcttc ggcgggctgt agccgtcgtc
     1861 cagcgagtca tagacgatct tgcggccgcc aacgccgccc tggtcgttga tcatcttgaa
     1921 tacggcggcc tcggtgcggg cgatgatgcc ataggcggag gcaggtccgc tgtaggctgc
     1981 ggtattgccg attttcagtt ccgtggcggt cacgccgggg gtttcctccg cagcacggac
     2041 gtgacgcggc aggcaggcgg cggccagccc ggccaggaa
//
LOCUS       DASWQN010000214         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_23016, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000214 DASWQN010000000
VERSION     DASWQN010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23016"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..962
                     /locus_tag="VGC82_03510"
     CDS             222..962
                     /locus_tag="VGC82_03510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0422371.1"
                     /translation="MIEETRDIQTRAGEMETFIVHPDRNGPHPVVFILMDAPGIREEL
                     YDMARRLCTVGYYVVLPNLYYRAGRDTKYGPDVTTKGSAEQARMRAVRTKMTIPPVMQ
                     DFADMLTFVDTQPAAKKGPVGTHGYCMSGPYSLAAAARYPDRVAAAASFYGTWLISEN
                     EESPHLSLAKAKGELYIACAEHDELAPLPMVAELKALFDKSGNSGELEMYPEVHHGFA
                     FPQRWCYDKPAAERHWERLIALYRRRLG"
     gene            969..>2135
                     /locus_tag="VGC82_03515"
     CDS             969..>2135
                     /locus_tag="VGC82_03515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0422372.1"
                     /translation="MTHMELWNHCENPYPFVPAEVLDAAESVRASLPNKYCDPEIAAT
                     LYDEVFDEYQLCDDIGLNIVTNEHHSGINNLWAASPVITGIVARATKKVRILSLGTLI
                     TVRPDPVRVATEYATIDVISRGRLDIGFVKSGATEMVSGDANPMRIREREWEAIDLIE
                     KSLTSHDGPFSWEGKFFTHPHVNIWPRPYQQPRPEFWAATSDMPTCAELGRRGIVNTL
                     LFGGYDRTRQAFDTYKQARRAAGLPAPTEDRFSYMAFCYVGDTDEEALRIGQKIPWFL
                     TVSIKSAPQMSKFMPGQLPPEMTPGAWRAGASKRPTNLTVEALIAQGQMFAGNPDTVV
                     KQIKAFRERVGGVGRIIMMTRQGLVTHAEAMKSFNLAAKEVLPQLRDLPPIEQRA"
BASE COUNT          454 a          730 c          604 g          347 t
ORIGIN      
        1 ggctccacat ctgtccgacc tgagaggagt tgtgcgtggt tgatgcacac gccgccgcac
       61 accggaagcg atgccgccaa gacagtagcc cgaacccatt ccggaacgcc ccgaacctcc
      121 agatcatcca tatcgatacc gccgcaagaa gcctccccac ccaagcttgc cccaaaccac
      181 ccatcgtgga accatcgcac caagcacgag ggaacacggc catgatcgag gaaacccgcg
      241 acatccaaac ccgcgccggt gagatggaaa cctttatcgt ccatcctgac cgcaacggcc
      301 ctcatcccgt ggtcttcatc ctgatggatg ctcccggcat ccgtgaggaa ctctacgaca
      361 tggcccgccg cctctgcacc gtcggctact acgtcgtgct gccaaacctg tattaccgcg
      421 ccggccggga cacgaaatac ggccccgacg tcaccaccaa aggcagcgct gaacaggctc
      481 ggatgcgggc ggttcgcacc aagatgacca tcccgccggt gatgcaggac ttcgccgaca
      541 tgctgacgtt cgtcgatacc cagccggcgg caaagaaagg tcccgtcggc acccacggct
      601 actgcatgag cggcccgtat tcgcttgccg ccgccgcgcg ctatcccgac cgcgtggccg
      661 ccgccgcatc gttctacggc acctggctga tcagcgagaa cgaggaaagc ccccacctgt
      721 cgctggcaaa agccaaaggc gagctctaca tcgcctgcgc cgaacacgat gaactcgcac
      781 cgctgccgat ggtagctgaa ctgaaggcgc tgttcgacaa atccggcaac agcggcgaat
      841 tggagatgta tcccgaagtt caccatggct tcgccttccc gcaacggtgg tgctatgaca
      901 agccagcggc ggaacgccat tgggagcgtc tgatcgcgct gtatcgccgt cgcctgggct
      961 gaccgacaat gacccacatg gaactctgga accactgcga gaacccgtat ccgttcgttc
     1021 ccgccgaagt gctcgatgcg gcggaatcgg tgcgggccag cctgccgaac aaatactgcg
     1081 atccggaaat cgcggcgacc ctgtatgatg aagtattcga cgagtaccag ctttgcgacg
     1141 atattggctt gaacatcgta acgaacgaac atcattcagg catcaacaac ctttgggccg
     1201 ccagcccggt cattaccggg attgtcgccc gggcgacgaa aaaagtccgc atcctgtcgc
     1261 tggggacatt gatcactgtc cgccccgatc cggtgcgggt cgctacagaa tatgccacga
     1321 tcgacgtgat cagccgcggt cggctggaca tcggtttcgt caagtcgggc gccaccgaaa
     1381 tggtatcggg cgacgccaac ccgatgcgca tccgcgagcg cgaatgggaa gccatcgacc
     1441 tgatcgaaaa atccctcacc tcgcacgatg gcccgttcag ttgggagggc aagttcttca
     1501 cccacccgca cgtcaacatc tggccgcgcc cttaccagca gccgcgcccg gagttctggg
     1561 ccgctaccag cgacatgccg acctgtgccg aactcggccg ccgcggcatc gtcaacaccc
     1621 tgttgttcgg cggctacgac cgcaccaggc aggcgttcga cacctataag caggcgcgtc
     1681 gcgcggccgg gctgcccgcc cccactgagg accgcttttc ctacatggca ttctgctacg
     1741 tcggcgacac cgatgaggaa gccctgcgaa ttggccagaa gatcccctgg ttcctgaccg
     1801 tcagcatcaa aagcgcgccg cagatgtcga agttcatgcc cggtcagttg ccgccggaaa
     1861 tgactcccgg agcatggcgg gccggagcct ccaagcgccc caccaacctg acggttgaag
     1921 ccctgatcgc ccaaggccag atgttcgccg gcaaccccga caccgtggtg aaacagatca
     1981 aagcgttccg cgaacgggtg ggcggcgtcg gccgcatcat catgatgacc cgtcaaggcc
     2041 tggtgacgca cgccgaggcg atgaagagct tcaaccttgc ggccaaggag gttctgccgc
     2101 aactgcgcga cctgccgcca atcgagcagc gggcc
//
LOCUS       DASWQN010000215         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1210326, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000215 DASWQN010000000
VERSION     DASWQN010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210326"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VGC82_03520"
     CDS             <1..944
                     /locus_tag="VGC82_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR04295 family B12-binding domain-containing
                     radical SAM protein"
                     /protein_id="HEY0422373.1"
                     /translation="TLRKLGVDAVVLGECEHVVAALAEAADWSRVASIALPGGDGPTI
                     NGGPAASPFVDLPPLRWPDEWVARHQHHHHRFDTPSVGPGAEVEASRGCPYNCSFCAK
                     IDFRDQYRRRDLTILLNEIDGLIVQGVRYLYFIDEIFLPQKPLLEALATRDVAFGVQT
                     RIDLWKPDMLRLLGSAGCVSIEAGIESLTVEGREALAKRCRLETEDLALLLIEARRHV
                     PFVQANLIGVDQDEATLVDYWRNRLIAKGVWANAPVPLYPYPSSPSYRAIWGDPDDAA
                     WERAHEHYLRTFAAFSDIQDDRPVPLALLETSCCGGH"
     gene            926..2029
                     /locus_tag="VGC82_03525"
     CDS             926..2029
                     /locus_tag="VGC82_03525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0422374.1"
                     /translation="MLRRPLIPLRRVLITLDAVGGIWRYALDVALGLEAYDVTCLLAV
                     FGPEPSAAQQAECKALPNADLVCVHEPLDWMVTDAAQLEPGVEKLAGLARDWKVDLLH
                     LNLPSQAAGLDSDVPVVVASHSCVPTWWEAVHGTALPETLEWQRDRNRQGFRQADAIM
                     VPSASHGAALRCAYGPLPPLHVVYNATTAAPGRHPKETFILSVGRWWDTGKNGQILDA
                     AAKLLPWPVVLAGPLNGPDGQATAFHHARTLGALAHEDILSLMERASIFAGPSRYEPF
                     GLAVAEAAASGSALVLADIATFRELWDGAALFVGADDTAEWANAFSTLIENEPLRWQL
                     AADAQSRAQRFTLPRQAARLYELYSELGAAAMT"
     gene            2026..>2311
                     /locus_tag="VGC82_03530"
     CDS             2026..>2311
                     /locus_tag="VGC82_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0422375.1"
                     /translation="MKVVLYAHSIVSDWNNGNAHFLRGILSELERRGHSTLALEPRDG
                     WSRSNLIADQGNGPLERFATSFPQLRVRTYTDDFDHEAALDDADVVIVHEW"
BASE COUNT          408 a          695 c          745 g          463 t
ORIGIN      
        1 ccaccttgcg caaattgggt gtggacgcgg tggtgcttgg cgagtgcgag catgtcgttg
       61 ccgccttggc cgaggcggcg gattggtcgc gcgtcgcctc catcgcgctg cccgggggtg
      121 acggtcccac gatcaacggc ggtccagcgg caagtccctt tgtcgatctt ccaccgctgc
      181 gctggcccga tgaatgggtg gcgcgtcatc agcaccacca tcatcggttc gatacgccgt
      241 cagtcggacc gggtgccgaa gtcgaggcat cgagaggctg cccctacaac tgtagcttct
      301 gcgccaagat cgacttccgc gaccagtatc ggcgccgcga cctgacaatc ctgttgaatg
      361 agattgatgg actgattgtc cagggcgtgc gctacctgta cttcatcgac gaaatcttcc
      421 tgccgcaaaa accgctgctg gaagcgttgg ccacacgcga cgtggcgttc ggggtgcaga
      481 cccggatcga cctgtggaaa cccgacatgt tgcggctgtt gggttcggct ggatgcgtgt
      541 caatcgaagc tggcatcgaa agcctgactg tcgagggccg tgaggccctg gcgaaaagat
      601 gccgactgga aacggaagat ttggcgttgt tgctgatcga ggctcggcgg cacgttccct
      661 tcgtccaggc caacctgatt ggagtggacc aggacgaagc gactctggtg gactattggc
      721 gcaaccgatt gattgcaaaa ggggtttggg ccaacgcacc ggtgccgctg tatccctatc
      781 ccagctcgcc aagttaccgg gcgatatggg gcgatccgga cgacgcggcg tgggaacgcg
      841 cccacgagca ttatctgcgc acgttcgccg ctttcagcga catccaggat gatcgtccgg
      901 ttccgctggc tttactggag acatcatgct gcggaggcca ttgatccccc tgcggcgcgt
      961 tttgatcacg ctcgacgcag tcgggggcat atggcgctac gcgcttgatg tggcgctcgg
     1021 ccttgaagct tatgatgtga catgtctgct ggcggtattc ggtcccgaac cgagcgctgc
     1081 gcagcaggcg gaatgcaagg ctttaccaaa tgccgacctc gtttgcgtgc acgagccgct
     1141 cgactggatg gttacggacg cggcgcagct ggaaccgggc gtggaaaagc tggctggctt
     1201 ggcacgcgac tggaaagttg acctgttaca cctgaatctc ccgtcccagg cagccggact
     1261 ggattccgac gttccggtgg tcgttgcgtc acactcgtgc gtgccgacct ggtgggaagc
     1321 tgttcacggc acagcgctgc ccgagaccct ggagtggcag cgggaccgca accgccaagg
     1381 gttccgccag gccgatgcga tcatggttcc cagtgcaagt catggcgctg cgcttcgttg
     1441 cgcttatgga ccgttaccgc cgcttcacgt ggtgtacaac gccacgaccg ccgcgccggg
     1501 ccgccatccc aaggagacat tcatcctgtc ggtcggccgc tggtgggaca ccggcaagaa
     1561 cggccaaata ctggatgcag cggcgaagtt gcttccgtgg ccggtcgtgc tcgcgggtcc
     1621 tttgaacggc cctgacggtc aagccacggc cttccatcat gcccgaacgc tgggcgcgct
     1681 tgcgcacgag gatattctct cgttgatgga acgagcgtcg atcttcgccg ggccgtcgcg
     1741 ctacgagccg tttgggctgg cagtggccga ggccgcggcc agtggcagtg cgctcgtcct
     1801 cgccgatatc gccacattcc gtgagctttg ggacggcgcg gctcttttcg tcggtgcaga
     1861 cgatacagca gaatgggcga acgcgttctc caccttgata gaaaatgaac cgttgcgctg
     1921 gcagctagcc gcggacgcgc agtcccgtgc acagcgattc acgctgcctc ggcaggcggc
     1981 gcggttgtac gaactttatt cggaactcgg cgccgcggcg atgacatgaa ggtcgtgctc
     2041 tatgcccatt cgatcgtttc tgactggaac aacggcaacg cccacttcct gcgcggcatc
     2101 ctgagcgaac ttgagcgtcg tggtcattcg accctggcgc tcgaaccgcg ggatggatgg
     2161 agccgttcga atctgatcgc tgatcaaggc aacggcccgc ttgaacgatt tgccacctcg
     2221 ttcccgcagt tgcgtgtcag gacctacacg gacgacttcg accacgaggc ggcactggac
     2281 gatgccgatg tcgtgatcgt ccacgaatgg a
//
LOCUS       DASWQN010000216         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_682236, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000216 DASWQN010000000
VERSION     DASWQN010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682236"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VGC82_03535"
     CDS             complement(<1..414)
                     /locus_tag="VGC82_03535"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY0422376.1"
                     /translation="MQALTFFFAFVAVLALIGVAAWLVRRFAGNRLGANPNRGRMPRL
                     AVIDAAAVDNRRRLVLVRRDNIEHLLMIGGPTDIVVEANIVRATAAREQIPQRNGGGA
                     EPPPRITPLPDAAIWTDDPLDLPEPQTSEPPPRPVR"
     gene            822..1205
                     /gene="flgB"
                     /locus_tag="VGC82_03540"
     CDS             822..1205
                     /gene="flgB"
                     /locus_tag="VGC82_03540"
                     /inference="COORDINATES: protein motif:HMM:NF004654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEY0422377.1"
                     /translation="MSALRTKMQWHQERQRVLSENVSNSDTPNFKARDLVEPKFDSAG
                     AATGAIGSLAMMRTSASHITPSGGGGQSFGQDRKTGFQTRPAGNAVSLEEEMLKVSAN
                     QMDYAAATSLYSKSLHLLKTAIGKA"
     gene            1237..1662
                     /gene="flgC"
                     /locus_tag="VGC82_03545"
     CDS             1237..1662
                     /gene="flgC"
                     /locus_tag="VGC82_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEY0422378.1"
                     /translation="MANDTGDFARSMGIATSGLRAQAGRMRVISENIANAESTASTPG
                     GDPYRRKVPTFSSELDRTLDARTVALGRVRPDPSAFRIKHEPGSPAADAAGNVKYPNV
                     NSLIEMTDMRDAQRSYEANLNIIGATRRMIQRTLDILKA"
     gene            1680..1988
                     /gene="fliE"
                     /locus_tag="VGC82_03550"
     CDS             1680..1988
                     /gene="fliE"
                     /locus_tag="VGC82_03550"
                     /inference="COORDINATES: protein motif:HMM:NF001949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEY0422379.1"
                     /translation="MASPTVAANAYASLARILDSGGAGKGTESGGPSFGAVLKDAIGS
                     VLDAGKKSDAQTVAMASGKANVMDVVTAVAETDVAVSTLVSVRDRVIQSYEDIMKMPI
                     "
BASE COUNT          398 a          661 c          627 g          332 t
ORIGIN      
        1 ccggaccggg cggggcggcg gctccgacgt ttgtggctcc gggagatcca gcgggtcgtc
       61 ggtccagatg gccgcatcgg gcagcggcgt gatgcggggc ggcggttccg caccgccgcc
      121 gttccgctgc ggtatttgtt cccgcgccgc cgtcgcacgc acgatattgg cctccacgac
      181 gatgtcggtc ggaccgccga tcatgagcag atgttcgatg ttgtcgcggc gcaccagcac
      241 cagccgccgg cgattgtcca ccgccgcggc gtcaatgacg gcaaggcgcg gcatccggcc
      301 gcgattggga ttggcgccga gtctgttgcc ggcaaatcgg cgcaccagcc aggcggcgac
      361 gccgatcagc gccagaacgg ccacgaatgc aaaaaagaat gtcagtgcct gcatgctctg
      421 tccccggcaa ccagcgcctt ctcacgtcct cgttgctcgc acgcgaatcg gagcgccacg
      481 gcagaccgcg aaaaagcctg agatcggcct tagtctgtta actttccacg gcagaaactg
      541 ccgccccgta tccttaatag cccatgaatc ggctgggcca aaacgacttc tctcagctcc
      601 ctacccaggc tgtttgcgtg gtaaacgcag gcatcgtggc gaaaatgggc acggaccgcc
      661 ttcgatgcgg cgaattcgcg ccggcatgcc gcgctatcca cgaccaatga tattttaacc
      721 agtcgttaac catgcacacg gcaaattctg cctagccggg cgggagtcgt gccgtccggc
      781 aaagggagcg gccttcgatg tccatcagcg atcttccggt gttgtcggcg ctgcgcacca
      841 agatgcaatg gcatcaggag cgtcagcgcg tcctgtccga aaacgtctcc aactccgata
      901 ccccgaactt caaggcgcgc gatctggtcg agccgaaatt cgattcggcc ggcgccgcca
      961 ccggcgcaat cgggtcgctg gcgatgatgc gcacctcggc cagccatatc acgccctcgg
     1021 gcggcggtgg gcagagcttc gggcaggatc gaaaaaccgg attccagacc aggccggccg
     1081 gcaacgcggt cagcctcgag gaggagatgc tgaaagtatc cgccaaccag atggactacg
     1141 cggccgccac ctcgctctac agcaagagcc tgcatcttct gaaaaccgcg atcggcaagg
     1201 cttgagcgcc ggaccgagac ctgaggagac gaagtcatgg caaacgatac aggggatttc
     1261 gcccgctcga tgggcatcgc cacctccggc ctgcgcgccc aggcggggcg gatgcgcgtg
     1321 atctcggaga acatcgccaa cgcggaatcc accgcctcga cccccggcgg cgatccctac
     1381 cgccgcaagg ttccgacatt ttcgtccgaa ctcgatcgca cgctcgatgc caggaccgtg
     1441 gcgctcggca gggtccggcc tgatccttcc gcgttccgca tcaaacacga acccggcagt
     1501 ccggcggcgg atgccgccgg caatgtcaaa tatcccaacg tcaattcgct gatcgaaatg
     1561 accgacatgc gcgacgcgca gcgatcctac gaggctaatc tcaacatcat cggcgccacg
     1621 cgccggatga tccaacgcac gctcgacatc ctcaaggcct gaaaagaaag ctttaagcca
     1681 tggcttcacc cactgtcgct gcaaacgcct acgccagtct cgcccgtatc ctggattccg
     1741 gcggcgccgg caagggcacc gaatcgggcg gtccgtcgtt cggcgcggtg ctcaaggacg
     1801 cgatcggaag cgtcctggac gctgggaaaa aatccgacgc gcagacggtc gcgatggcgt
     1861 cgggcaaggc caatgtgatg gacgtggtca cggcggtggc ggagaccgat gtcgcggtct
     1921 cgacgctggt gtcggtgcgt gaccgggtga tccagtcata cgaagacatc atgaagatgc
     1981 cgatctgacg tcgctagacg tcatcccggg gcgatgcg
//
LOCUS       DASWQN010000217         4485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1474207, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000217 DASWQN010000000
VERSION     DASWQN010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474207"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VGC82_03555"
     CDS             complement(<1..329)
                     /locus_tag="VGC82_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0422380.1"
                     /translation="MNHFLLNLALAVIPVVVAITLHEAAHGYAALALGDTTARDAGRL
                     SLNPLKHIDRVGTIILPGFLLISQLLVPPHKIFFMFGWAKPVPIGAWKFRDPRRGMAL
                     VAIAGPL"
     gene            complement(326..1345)
                     /gene="nagZ"
                     /locus_tag="VGC82_03560"
     CDS             complement(326..1345)
                     /gene="nagZ"
                     /locus_tag="VGC82_03560"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY0422381.1"
                     /translation="MTETAGLDPTRAAIVGIAGPILLADESLLFKAFPPAGIILFSRN
                     IQDPAQLGSLIATLRTVLPADADIMVDQEGGRVARLRPPHWHGHPAAAALGRLFERDP
                     SAGLRAAWLTGALIGTDCRDAGFTIAATPVLDISVLAAHDVVGDRAFARHPVSVARLG
                     RAVANGLLAAGITPVGKHAPGHGRAQVDSHLVLPKVAEDDLSADIRPFALNADLPWMM
                     TAHIVYATLDPGLPATLSAAVIGGVIRGRIGFEGVLVTDDLAMKALSGDPADLAVQAL
                     AAGCDIALYCSGAFAPTEALLRTCPSVTPATDQRLRAARAASASRGLKLDPVRLSAER
                     ERLLT"
     gene            complement(1563..2258)
                     /locus_tag="VGC82_03565"
     CDS             complement(1563..2258)
                     /locus_tag="VGC82_03565"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY0422382.1"
                     /translation="MFAGGLGAVLVALIGASALVGRHGGEVPVVTADARPVREKPLHP
                     GGMKIDGVENDVFSGQSDNTNARLSAAPEAPDTNGLRAAAVPAQPAVETPAQTAAARP
                     SPPASPAPLGKPAVVASMVPVAKPAPAKPAPTPGKPAPAAVVESHPGGHPATVQLAAL
                     SSEEAARNEWKQLSKRAPELLNGRQPSFQRVERDGHTVWRVRTSGFADVAQARIFCDH
                     ARTKGLGCSVAEF"
     gene            complement(2426..4216)
                     /gene="argS"
                     /locus_tag="VGC82_03570"
     CDS             complement(2426..4216)
                     /gene="argS"
                     /locus_tag="VGC82_03570"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY0422383.1"
                     /translation="MNPFDLYARVRETVLAALMEIVPDLPADIAARVEVTPTRDQSHG
                     DMATNAAMVSAKAARQPPAKIAAALVQHLNAGAQIETAESAGPGFVNLRLRPEAFRAL
                     LPIILESGEAYGDATTGSGTKVNVEYVSANPTGPMHIGHCRGAVVGDALANLLIKAGF
                     EITKEYYINDAGAQVTALAWAAYWRYLQAIGSPMTEHEFAEEVPGGLQYKGEYLVSVG
                     QTLAARFQASLATPDLGIASPEVWLNAVKDLTIDAMMTEIREDLRLLGVQQGVFSSER
                     ALVASGAADAIIEKLHADGLIYQGVLEPPKGKTPDDWEARPQTLFRSTDFGDDIDRPL
                     RKSDGSNTYFANDIAYHADKIARGYDVLINVLGADHGGYVARMKAAVRALSADSVKLE
                     AVLCQIVRVVRGGKTVPMSKRAGTYVTLRDLLDEVGKDAVRFTMLTRKSDAQMEFDLD
                     QVLATTRENPVFYVQYAHARCRSVLRAAAEMFPNQTITAHGLAKVSVDTLASEPELAL
                     IRRLGVWPRIVEAAAIAREPHRIAFFLYDLAADFHMLWNRGKDDATLRFLQADRPAET
                     LARLALVAATAVVIRSGLAVMGVEPVEEMR"
BASE COUNT          741 a         1399 c         1542 g          803 t
ORIGIN      
        1 atcagcggcc cggcgatcgc gacgagcgcc atgcctcgcc tggggtcgcg gaatttccac
       61 gcgccgatcg gcaccggctt ggcccagccg aacatgaaga agatcttgtg cggcggcact
      121 agcaactggc tgatcagcag gaaacccggc aggatgatcg tgccgacgcg gtcgatatgt
      181 ttcagcgggt tgagcgacag ccggccggcg tcccgtgcgg tcgtgtcgcc cagcgccagc
      241 gccgcgtagc cgtgcgccgc ctcgtgcaag gtgatcgcca cgacgaccgg aatgaccgcc
      301 agcgccaggt tcagcagaaa atggttcacg tcagcagccg ttcccgttcg gccgacaatc
      361 ggaccgggtc cagcttcagg ccgcggctcg cggaagcggc gcgtgcggcg cgcagccgct
      421 ggtccgtggc cggggtaacc gatggacagg ttcgcagcaa agcctcggtg ggagcgaacg
      481 cgccgctgca atacagcgcg atgtcgcagc cggcggccag cgcctggacc gccaggtcgg
      541 cggggtcgcc ggagagtgcc ttcatcgcca ggtcgtcggt caccaaaact ccttcaaagc
      601 cgatccgccc gcggatgacg ccgccgatga cggccgccga caaagtcgcg ggcaacccgg
      661 gatcgagcgt cgcgtaaacg atatgggctg tcatcatcca tggcaggtcg gcgttcagcg
      721 cgaaaggccg gatgtcggcg gacaagtcat cttccgcaac cttcggcagc acaagatggc
      781 tatcgacctg agcccgccca tgtcctggtg catgctttcc gaccggcgtg atgccggcgg
      841 ccagcagtcc attcgcgacc gcccggccca gacgagcgac cgagaccgga tgtcgtgcga
      901 acgctcggtc accgacgaca tcatgcgccg cgaggaccga gatatccagc accggcgtgg
      961 cggcgatggt gaagccggcg tcgcggcagt ccgttccgat cagtgccccg gtcagccagg
     1021 ccgcgcgcaa tcccgctgac ggatcgcgtt cgaacaaccg tcccaacgcc gcggcggccg
     1081 gatggccgtg ccagtgcggc gggcgcagcc gggcgacacg gccgccctcc tgatcgacca
     1141 tgatatcggc gtccgccggc agcaccgttc gcagcgtggc gatcaacgat cctagttgtg
     1201 ccggatcctg aatgttgcgg gaaaacagga tgatgccggc gggcgggaaa gccttgaaca
     1261 gcagggattc atcagcgagc aagatgggcc cggcgatgcc gacgatggcg gcacgagtgg
     1321 gatctaatcc ggccgtttcc gtcacggcat cacctgctca caccccacaa cgcttttcgc
     1381 tcatgtgcgt ttgaagcatt ttcagcctgc ccatacggcc cgaaaatgct tcatctcctt
     1441 gttcggtcgc atgattcacc cgctttggac gttccgccca gcgtgatatg ctccatctcc
     1501 ttgtttggtc gcatgattca cccgctttgg acgttccgcc cagcgtgatc atgctcaaat
     1561 gctcaaaact ccgctacgga acagccgagg cctttcgtgc gcgcgtggtc gcagaagatc
     1621 ctggcttgtg ccacgtccgc gaatcccgag gtccggaccc gccacacggt atggccgtcc
     1681 cgttcgacgc gctggaagct gggttgccgg ccgttcaaca gctcgggcgc ccgcttcgac
     1741 agttgcttcc actcgttgcg cgcggcctct tccgatgaaa gcgcggcgag ctgcaccgtc
     1801 gccgggtgtc cgccgggatg gctttccacg acggccgccg gagctggctt tcccggggtg
     1861 ggcgcaggct tggcgggagc aggtttggcg acgggaacca tgcttgcgac gacggccggc
     1921 ttgccgagcg gtgccggtga agcgggcggc gatggccgcg ccgcagccgt ctgggcaggc
     1981 gtttcgacgg ccggctgggc gggcacggcg gcagcacgca agccgttggt gtctggagct
     2041 tccggcgcgg ccgacaatct ggcgttggtg ttatccgact gaccggagaa aacatcgttc
     2101 tccacgccgt cgatcttcat gccgccggga tgaagcggct tttcccggac ggggcgagca
     2161 tcggcagtga cgacggggac ttcgccaccg tgccttccca ccagcgccga ggcgccgatc
     2221 aaggccacca acacggcgcc gagtccgccg gcgaacatca ccaggcggcg ggtgtccgga
     2281 tccatgcccg gtctggctct ggcctggccc gtgccataac gtccagcctg gtttccgcgg
     2341 tagccggaat tcgggatgct tagatcgtct gacatagtct accgttcttt cccgcttggc
     2401 ctcggccggc gggatcgacc ggctttcagc gcatttcctc gactggttcg acccccatca
     2461 ctgcgagacc ggatcgaatt accacagccg tcgccgccac cagcgcaagt cgcgccaatg
     2521 tttcagccgg tcggtccgcc tgcaggaatc ttagtgtcgc gtcgtccttg cctcggttcc
     2581 atagcatatg aaagtcggcc gccaagtcat agagaaaaaa tgcaattctg tgcggttctc
     2641 tggcaatcgc cgcggcttcg acgatgcgcg gccaaacacc gaggcggcgg atcagcgcca
     2701 gctccggctc gctggcgagc gtgtccacgg ataccttggc gagaccatgg gcagtgatgg
     2761 tctgattcgg aaacatctcc gcggcggcac gtaagaccga acggcaacgg gcgtgggcat
     2821 attgcacgta gaagaccggg ttctcccgcg tggtggcgag aacctggtcc aggtcgaact
     2881 ccatctgggc gtcggacttg cgggtcagca tggtgaagcg gacggcgtcc ttgccgacct
     2941 cgtccagcag gtcgcgcagg gtgacatagg tgccggcgcg tttggacatg ggcacggttt
     3001 tgcccccgcg cacgacgcgg acgatctggc agagcaccgc ttcgagcttg acgctgtcgg
     3061 ccgaaagggc gcggacggcg gctttcatgc gggcgacgta gccgccgtgg tcggcgccca
     3121 ggacgttgat cagcacatcg tagccgcggg cgatcttgtc ggcgtggtag gcgatgtcgt
     3181 tggcgaaata ggtgttgctg ccgtcggatt ttcgcaacgg acgatcgatg tcgtcgccga
     3241 aatcggtgga acggaacagg gtttgcgggc gggcttccca gtcgtctggc gtcttgccct
     3301 tgggaggctc caggacgccc tggtagatca ggccgtcggc gtggagtttc tcgatgatcg
     3361 cgtcagccgc gcccgatgct accaaggccc gttcgctgct gaagacgccc tgctggacac
     3421 cgagcaggcg aaggtcttcg cggatctcgg tcatcatggc gtcgatcgtg aggtctttga
     3481 ccgcgttcag ccaaacctcg ggcgatgcga tgcccagatc cggggtagcg agggaggcct
     3541 ggaagcgcgc agcgagggtt tggccgacgc tgaccagata ctcgcccttg tattgcaggc
     3601 cgccagggac ctcctcggcg aattcgtgtt cggtcatcgg cgagccgatg gcctggaggt
     3661 accgccaata cgccgcccag gcgagggcgg tgacctgtgc gccggcgtcg ttgatatagt
     3721 actccttggt gatctcgaat ccggctttga tcagcagatt ggcgagggca tcgccgacca
     3781 cggcgccccg gcaatggccg atatgcatcg ggcctgtggg gttggccgag acatactcga
     3841 cgttgacctt ggttcccgaa ccggtggtgg cgtcaccgta ggcttccccc gattccagga
     3901 tgatcgggag cagggcacgg aaggcttccg ggcggaggcg aaggttgacg aagccgggtc
     3961 cggcggattc ggctgtctcg atctgtgcac cggcgttcag gtgctgaacg agggcggcgg
     4021 cgatcttcgc gggcggttgg cgcgcggctt tggccgagac catggcggcg ttggtggcca
     4081 tgtcgccgtg ggattggtcg cgggtggggg tgacctcgac gcgggcggcg atgtccgccg
     4141 gcaggtcggg gacgatttcc atcagggcgg ctaggacggt ctcgcgcacg cgagcgtaga
     4201 ggtcgaacgg gttcatgacc gggtgttagc gcgaacgggc aggcgggtcg atgattttgg
     4261 cgccggctat ggcggctcgg ggattttgca gccggcaaca gcggttcggg gatttggcgc
     4321 cggcgccggc ggtttggctg cagaatcccc cgcttcgaag aggcatcggg gtgcggcatg
     4381 attcgcagca gcctgatcgg cgcccggagg cgatggtccg gcggtgacat cggcgttctc
     4441 acgcggagtt ttatttaccg atcgcgcata aaaccggtat cagca
//
LOCUS       DASWQN010000218         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2134249, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000218 DASWQN010000000
VERSION     DASWQN010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134249"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="VGC82_03575"
     CDS             complement(<1..1646)
                     /locus_tag="VGC82_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422384.1"
                     /translation="MANGFNDIPDWFSSENQGAGVAVAQNDLVVLMVDHPGQQPNRGL
                     YRLGKNLDNTGKVTGAWTPWIDIPDWFSFENQGADIAIADLFNNGGRDLVVLMVDNPP
                     QQNRGLYRIGRGLDGNGAVTGGWTPWIDVPEWFSWENQGAGVAVTPRDAQGKCHLIVF
                     MIDNPPEVNRGLYRIGRDLAADGTVQGGWSAWTDVTGWFSWENQGGGVAAIDLVRNGS
                     LDLVTFQIDNALPTEDAGGKNEAFFRIDKGLSLDGPVQIPATDWLGVPHWFAWENQYG
                     SIAIAELAGTRKLFVLMVDNPAGRNGGFYTTVDIDQAPAVYGKWDVLPFHSGVLAVHA
                     ALLPTGDVLFFAGSGSSEVRFRSPDFGDVAKGVPVSVVWTPEGNDFFHPNTLRTANGR
                     PFDVFCGGDAFLPDGRMLSAGGTQDYNNFKGRADAIIFDPKTRQWTFTSHMAHARWYP
                     SLMTLGDGRVLATTGLDENGFEGSKNTMEAYDAGTKTWNPLHFAAGVSLPLYAHLFQL
                     QDGRIFFSGGRMDDPMNAQPGIFDLTRNPVPFDPVPDLLAP"
     gene            complement(1854..3362)
                     /locus_tag="VGC82_03580"
     CDS             complement(1854..3362)
                     /locus_tag="VGC82_03580"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HEY0422385.1"
                     /translation="MSSPAVRRFHGGVRGAEMPRGSLLFEGRFGRMFRTLPAARFSDD
                     ALNALGAAMVAPPDDPPTPETETDAEENQGINAGYTYLGQFIDHDLTFDPASSLIKQN
                     DPQALTDFRSPRFDLDNVYGRGPDDQPYLYEPDGLHMQLGKPMTGNPNDGGTRDLPRH
                     TSLNGGAARALIGDPRNDENVIVSQLQSTMLRFHNRMVDLNPGVGLEEIQRLVRWHYQ
                     YVVLNDFLPTIVGIDMVQSVLPHLKSGRSILHDRPVLHFYKWRNEPYIPIEFSVAAYR
                     FGHSMVRPIYRLNTTIPRLAIFDPEGDNKSLTGFREFPSNWAVDWNLFFDMGNNAPAS
                     GPGRVQKAYKIDTSLVNPLGSLPPSVASNPSSLAARNLLRGLRMGLPSGQDVARAMGV
                     EPLRDDQLAVGKATKDDPPVAKGIAALPKFGDEFRGKAPLWYYVLAEAQRQFKTDATP
                     IRLGDVGGRIVMETFAGILLGDSHSFLSQNPTWTPGKPDFKMADLIKMAIGS"
BASE COUNT          689 a         1109 c         1085 g          718 t
ORIGIN      
        1 ggggccagca ggtccggaac cggatcgaac ggcacgggat tgcgggtcag gtcgaagatg
       61 ccgggctggg cgttcatcgg gtcgtccatg cggccgccgg aaaagaagat ccgcccatcc
      121 tgcaactgaa acagatgagc atacaaagga aggcttacgc cggcggcaaa gtgcagggga
      181 ttccaggttt tggtccctgc atcataagcc tccatcgtgt tcttcgagcc ttcgaagccg
      241 ttctcatcga ggccggtggt cgccagaaca cgcccgtcac cgagcgtcat caggctggga
      301 taccatctgg catgggccat gtgggaggtg aaggtccatt gccgcgtctt cggatcgaag
      361 atgatggcgt cggctcggcc cttgaagttg ttgtaatcct gggttccgcc ggccgagagc
      421 atccttccgt ccggcaggaa ggcatcaccg ccgcagaaca cgtcgaacgg ccgcccgttc
      481 gccgtacgca gcgtgttcgg atgaaaaaag tcgttgccct ccggtgtcca tacaacgctg
      541 accggcacgc ccttcgcgac atcgccgaaa tcgggcgagc gaaagcgcac ctcgctgctg
      601 ccggagccgg caaagaacag cacatcgccg gtgggcagca acgcggcgtg taccgccagg
      661 acgccggagt ggaatggcag cacgtcccac tttccataga ccgcgggcgc ctggtcgatg
      721 tccaccgtcg tatagaagcc gccgttccgg ccggccggat tgtccaccat caggacgaac
      781 agtttgcgcg tgccggcgag ttccgcgatt gcgatactgc cgtattggtt ttcccaggca
      841 aaccagtgcg gcaccccaag ccagtccgtt gcgggtattt gcaccggacc gtctagcgac
      901 agacctttgt cgatacggaa gaacgcttcg ttcttgccgc cggcatcctc ggtcggcaag
      961 gcgttgtcga tctggaaggt caccaggtcc agcgacccgt tccgaacgag gtcgatggcc
     1021 gcgactccgc cgccctggtt ttcccaggaa aaccagccgg tcacgtcggt ccacgccgac
     1081 cacccgccct gtacggtccc gtccgccgcg aggtcgcgtc cgatccgata cagcccgcgg
     1141 ttaacctcgg gcggattatc gatcatgaag acgatgagat ggcattttcc ctgggcgtcg
     1201 cgcggtgtca cggcaacgcc tgcgccttga ttctcccacg aaaaccactc gggcacgtcg
     1261 atccacgggg tccagccgcc ggtgacggcg ccgttcccat ccagcccgcg tccgatccga
     1321 tacagcccgc gattctgctg tgggggattg tccaccatca ggacaaccag gtcccggccg
     1381 ccattgttga acaggtcggc gatggcgatg tcagcccctt gattctcgaa ggagaaccag
     1441 tccgggatgt cgatccatgg cgtccaggcg ccggtgacct tgccggtatt gtccaggttc
     1501 ttgcccaacc ggtagaggcc ccgattgggt tgctggccgg ggtgatcgac catcaacacg
     1561 accagatcgt tctgggctac ggcgacgccg gccccctgat tctcgctgga gaaccagtcg
     1621 ggaatgtcgt tgaagccgtt cgccatagat atgctcccgc gttgccggtc cgcctgaagt
     1681 taaaggtttg atgacaagat aatcgtttcg agccagacgc atttccgcac aatgttccgg
     1741 cgggcgcagt cccatcatcc cgggctcgcg gtgtgcgatc tatccagggc gtggatggtg
     1801 agcctgcgcc ccccaagacg gtacccacca gcgtcagacg ggcggcaaca accttagctg
     1861 ccgattgcca tcttgatcag gtcggccatt ttgaagtccg gttttccggg cgtccatgtc
     1921 gggttctgcg acaggaacga atggctgtcg cccagcagga tgccggcgaa cgtttccatg
     1981 acgatgcggc caccgacgtc tcccagccgt attggcgtcg cgtcggtctt gaactgacgc
     2041 tgtgcttcag ccagcacata gtaccatagc ggtgccttgc cccggaactc gtcgccgaat
     2101 ttcggcaggg cggcgatacc cttggccacc ggcggatcat ccttcgtcgc cttgccgaca
     2161 gccaactgat cgtcgcgcag cggttcgacg cccatcgccc gcgcgacgtc ctgtccagac
     2221 ggcagaccca tgcgcagacc ccgcaacagg ttacgcgccg ccagcgagct tgggttggac
     2281 gcaacggaag gcggcaggct gccgagcgga ttgaccagcg acgtgtcgat tttgtaggcc
     2341 ttttgcaccc gcccgggtcc gctcgccgga gcattgttgc ccatgtcgaa aaacaggttc
     2401 cagtccaccg cccagttcga cggaaattcc cgaaagccgg tcagcgactt gttgtcgcct
     2461 tcaggatcga atatcgccag ccgcggaatc gtggtgttca ggcggtagat cggccgcacc
     2521 atggaatgcc cgaaccggta cgccgcgacc gagaattcta tcggaatata tggctcgttg
     2581 cgccatttgt agaagtgcag cactggcctg tcgtgcagga tggacctgcc gcttttcaga
     2641 tgcggcagca ctgactgcac catgtcgatg cccacgatcg tcggcaggaa gtcgttcagc
     2701 acgacatact ggtagtgcca gcgaaccagc cgctgaattt cttccaggcc aacaccggga
     2761 ttcaaatcca ccatgcggtt gtggaaccgc aacatcgtac tttgaagctg agaaacgatg
     2821 acattctcat cgttgcgcgg atcgccgatc aacgcccgtg cggcaccgcc gttcaacgac
     2881 gtgtggcgtg gcaggtcgcg agtgccgccg tcattcgggt tgcccgtcat tggcttccca
     2941 agctgcatgt gcagcccatc gggttcgtaa aggtagggct ggtcgtcggg gcctcgacca
     3001 taaacgttat cgaggtcgaa gcgaggcgaa cgaaagtcgg tcagggcctg gggatcgttc
     3061 tgcttgatca gcgaacttgc tggatcgaac gtcagatcgt gatcgatgaa ttggccgaga
     3121 taggtataac cggcattgat gccttgattt tcctcggcgt cggtttcggt ttccggagtt
     3181 ggcggatcat ccggcggcgc gaccatcgcc gcgcccaagg cgttcagcgc atcgtcggaa
     3241 aatctcgccg cgggcagcgt gcgaaacatg cggccaaatc tgccctcgaa aagcaaagac
     3301 ccgcgcggca tttcggcacc gcgcaccccg ccgtgaaacc ggcggactgc tggactagac
     3361 attggcccct ccgtgatatt ttttgtctcc cgatatgtat tgtcattctt tttgctgctg
     3421 gagccagcaa gatgccgcag tatcacctcc attttaccat tgcatatcgg aatatatcag
     3481 actagaaaac gaattgtggc ggtctggttt tggtgcggac gcccggccaa accgcttttg
     3541 ttgttatatc aaagcgacag ggcagcgatg cttccgcacc ggttgcgatg caggatcccg
     3601 t
//
LOCUS       DASWQN010000219         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1078422, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000219 DASWQN010000000
VERSION     DASWQN010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078422"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VGC82_03585"
     CDS             <1..563
                     /locus_tag="VGC82_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY0422386.1"
                     /translation="SDGRCCNTSLLIDRQGRIAARYDKLHLFDIGLAGQPTYRESAAV
                     SGGSELVTADIEGIVAGMSICYDLRFPEVYRLHALAGAQLLLVPAAFTLHTGRDHWEL
                     LLRARAVENQCFVVGAAQVGESIPGRSCYGRSMVVDPWGTVLACAPDTVGVIVADLDF
                     QRQHRLRSELPALANRRPDVYRLERR"
     gene            complement(639..2324)
                     /locus_tag="VGC82_03590"
     CDS             complement(639..2324)
                     /locus_tag="VGC82_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0422387.1"
                     /translation="MTRRTETHETDIVVVGGGSAGCVLAARLSENLGLRVLVVEAGRR
                     DRLGVTRLPAALLWTIGNPRYDWAYTSEPDPTRDGQTEFWPRGKTLGGSSAINGMIYI
                     RGAAADYNAWEAMGCSGWGWRDVLPLFRGLETADVPDDTHRGADGPQRVSALRWRHKL
                     AADFIASGVAAGIPPNDDLNGAVHEGIGWNQGSTIQGRRHSAYDAFLTPNLRRRNLSV
                     LDDLLVERLTFTGRRATGLHARRSDGTPLHVTARAGVVLAAGAINSPQLLMLSGIGDA
                     AALRRKGIVPVVDVPEVGQNLMEHPGLYVQAEMTEPTANRETRPLRAAWHFARWLARH
                     DGPMSVPTAQALAFFRSTPDMAEPDLQFHLFPYGSTLKNGKRVIPNRNLATILVNLNF
                     PKSRGWLELRTADPATPVAIHPRLLEHPDDIETLLHGLAWVRRMAATPPFGPKLRALI
                     GVPRQDAGPAADLAFLRTAARPFYHPAGTCRMGADDRSVVAPDLAVRGVDRLWVADIS
                     IFPRHIAGNTNATALMIGEKAASLIAGALQETSLHRNPAQASTVSRAESRVPA"
     gene            complement(2366..>2710)
                     /locus_tag="VGC82_03595"
     CDS             complement(2366..>2710)
                     /locus_tag="VGC82_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422388.1"
                     /translation="PARSAEVPGDVLPASGDRGTSGGCCAKCSWFDGAANGVGQHGKP
                     SADDETRWARSSGQRDGDPVIFEGRDGGAYSVMRSNPAKLLPDVGCYCLISINKKHSV
                     GSGVFLWTPPVF"
BASE COUNT          493 a          889 c          834 g          494 t
ORIGIN      
        1 aatccgatgg acgctgttgc aacaccagcc tgttgatcga ccggcaggga cgcatcgcgg
       61 cccgctacga caagctgcac ctgttcgaca tcggtttggc gggtcagccc acctaccgtg
      121 aatccgccgc cgtgagtggt ggcagcgagc ttgtgaccgc ggacatcgaa ggcatcgttg
      181 cagggatgtc catctgctac gatctgcgct tccccgaagt ctatcgcctg cacgctctgg
      241 ccggagcaca gctactgctc gtacccgccg cctttacact acacaccggg cgcgaccact
      301 gggagctgct gctgcgggcc cgcgccgtgg aaaaccagtg ctttgttgtc ggcgccgccc
      361 aggtcggaga atcgatcccg ggacgatcgt gctatggtcg cagcatggtc gtggatccct
      421 ggggcaccgt tctcgcgtgt gcgccggata ccgtgggcgt gatcgtcgcg gacctcgatt
      481 tccaacgaca gcacaggctt cgctcggagc ttccggccct cgctaaccgg cggccggacg
      541 tttatcgctt ggaaaggcgt taggatcggg gttccggctc atccatccga ccttgatcgc
      601 cgccttgtct ggccctttct acacgaccat cccaatgatc acgccggcac ccgagattcc
      661 gctcgcgaaa ccgtggatgc ctgcgccggg tttcgatgca gtgaggtctc ttgcaacgca
      721 ccggcgatca gggatgctgc cttttcgccg atcatcaacg ctgtcgcgtt cgtattgcca
      781 gcgatgtgcc gggggaaaat cgagatgtcg gctacccaaa gccggtcaac cccccgcacc
      841 gcgaggtccg gggctacaac cgaacggtca tcggctccca tccggcaggt accggcggga
      901 tggtagaacg gccgtgccgc ggttcgcagg aaggcaaggt cggccgcggg cccagcgtcc
      961 tggcgcggca ccccaatcag cgcacgcagc ttcgggccaa acggcggcgt ggctgccatg
     1021 cggcgcaccc aagcaagccc gtgaagcaga gtctcgatgt cgtccggatg ctcaagcagg
     1081 cgtgggtgaa tcgcgaccgg cgtggccgga tcggcggtac gcagttcaag ccagccacgc
     1141 gacttcggaa agttcaggtt gaccaggatc gtcgcgaggt tccgatttgg aatcacccgt
     1201 ttgccgttct tcagcgtcga gccatacggg aacaaatgaa attgcaggtc cggttcggcc
     1261 atatcgggtg ttgaccggaa gaaggccagc gcttgcgcgg tcggcacgct cattgggccg
     1321 tcgtgccgcg ccagccagcg agcaaaatgc caggcggcgc ggagcggcct ggtttcccgg
     1381 ttggcggtgg gctccgtcat ctcggcctgc acatacaggc caggatgttc cataaggttc
     1441 tgcccgactt caggcacgtc caccacgggt acgatgccct tccgccgcag ggctgcggca
     1501 tcgccgatcc cggacaacat cagaagctgt ggggaattga tggcgccagc cgccagcacg
     1561 acccccgctc gtgcggtcac gtgcagcggc gtcccatcgc tgcggcgggc gtgcagcccc
     1621 gtggcgcgcc ggcccgtgaa ggtaagccgt tcgaccagca ggtcgtccag gaccgacaga
     1681 tttcggcggc ggaggtttgg tgtgagaaag gcatcatagg ccgagtgacg ccgcccttgg
     1741 atggtcgagc cctggttcca gccgatgccc tcatgcaccg ccccattcag gtcgtcgttc
     1801 ggcgggatgc cggccgcaac accgctggcg ataaaatccg ctgccagctt gtggcgccag
     1861 cgcagcgctg aaacacgttg cggaccatcg gcgccgcgat gcgtatcatc gggcacgtca
     1921 gccgtttcca ggccacggaa cagcggcagc acgtcgcgcc agccccagcc cgagcagccc
     1981 attgcttccc atgcattgta atccgctgcc gcgccgcgga tatagatcat accgttgatc
     2041 gccgaactgc cccccagggt tttgccgcgc ggccagaact ccgtctggcc gtcccgcgtc
     2101 ggatcgggtt cagaggtata agcccaatca tatcgagggt tgccgatggt ccaaaggagc
     2161 gcggcgggaa gccgggtcac cccgagtcga tcgcgtcgtc ctgcttccac aacgagcacc
     2221 cgaagaccca agttctcgga gagacgggcg gcgaggacac atccagcgct gccgccgccg
     2281 accacaacga tgtccgtctc gtgcgtttct gtgcgccggg tcatcacgcc tcgttctatt
     2341 cagtgacagg accataatgg ttccatcaaa ataccggagg tgtccataaa aatacgcctg
     2401 atcctaccga atgtttcttg ttgatactta tgagacaata acatcccaca tctggcagca
     2461 atttcgccgg attacttcgc atgactgaat aagccccgcc atctctgcct tcaaaaataa
     2521 ccgggtcgcc atcccgctgg ccggatgagc gtgcccagcg agtttcgtca tcagcggaag
     2581 gcttgccatg ctgccccacc ccgttggcgg cgccatcaaa ccagctgcat ttggcgcagc
     2641 agcctcccga ggtcccgcgg tctcctgatg ccggcaagac atcaccaggt acctcggccg
     2701 atcgggcggg
//
LOCUS       DASWQN010000220         1976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2266443, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000220 DASWQN010000000
VERSION     DASWQN010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2266443"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VGC82_03600"
     CDS             complement(<1..555)
                     /locus_tag="VGC82_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY0422389.1"
                     /translation="MDDLLSDFLTETNESLGELDVALITLERTPDDAETLSFIFRLVH
                     TIKGTCGFLGLPRLERVAHAGENILGKLRDRSLTVTPDIVTQVLSAIDQIKAIVTCLG
                     ATGAEPAGDDAELIAALNLTAANGIRSASTNRRRSNSISSPLRLKRLSKRSRRSGRKP
                     PPRQPAPNLPLLRPPRRRPPHLPLR"
     gene            895..1881
                     /gene="moaA"
                     /locus_tag="VGC82_03605"
     CDS             895..1881
                     /gene="moaA"
                     /locus_tag="VGC82_03605"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY0422390.1"
                     /translation="MIDPFGRAITYLRVSVTDRCDLRCVYCMAEDMTFLPKRDLLTLE
                     ELDRLCEAFIRLGTRKIRITGGEPLVRRDVMRLFGRLGDRLGHGLDELTVTTNGSQLV
                     KHAETLVAAGVKRINVSLDTLDPVRFAQITRWGKIEQTLDGIFAAKAAGLAVKINAVA
                     MKGVNEAEFDGMLAWCGEHGFDLCLIETMPMGDISGDRTDQYLPLSVVRARLRKIWTL
                     EDTDYKTGGPARYFTVKETGQRIGFITPMTHNFCESCNRVRLTCTGTLYMCLGQDDSA
                     DFRPLLRAGVSDDQLDDAIHEAISRKPKGHDFIIDRRHDKPAVARHMSVTGG"
BASE COUNT          365 a          576 c          689 g          346 t
ORIGIN      
        1 gcgaagcgga aggtgcggtg gccggcgccg cggcgggcgt agcagcggaa ggttcggcgc
       61 gggctgccgg ggcggcggtt tccgcccgct ccgccgcgac cgcttcgaca accgcttcag
      121 ccggagtggt gagctgatcg aattcgagcg ccggcggttg gtcgacgccg agcgaatgcc
      181 gtttgccgcg gtgagattca gtgcggcgat caattctgcg tcgtcaccgg cgggttcggc
      241 gccggttgcg cccaggcacg tcacgattgc cttgatctgg tcgatcgcgc tcagcacctg
      301 tgtgacgata tccggagtta cggtcaggct gcggtcgcgc agcttcccca ggatgttttc
      361 cccggcgtgg gcgacccgct cgagccgcgg caagcctaaa aagccgcaag tgcctttgat
      421 cgtgtgcacc agccggaaga tgaacgacag cgtttcggcg tcgtcggggg tgcgttccag
      481 cgtgatcagc gcgacatcga gctccccgag gctctcattc gtctcggtca ggaagtcgga
      541 aagaagatcg tccactttag cccccttggc agcccggtat ccgggcggga gccaagtctt
      601 ggcatcaatt cgcaaacaaa aagtaaatgc cggtgagatt tcgggtttca ggtgccatca
      661 aggcgcaagc gccaacccgg ccggatggat gcgggcgcca tcaaccgcgc atcgcgggaa
      721 ctcgcagccg accgctacag gcaccggggc ctgctccagc gttcggattg ggccgagacc
      781 cttgaacacc gggccgtgct ggcgcaagtc atggtgaacg ccgtataaca tcaggcaacc
      841 gataacggtc ggcaaccggc aaatgccggg gccgtcagga gatacaacca cattatgatc
      901 gatccgttcg gcagagcgat tacctacctg agggtttcgg tgaccgaccg ctgcgacctg
      961 cgctgcgtct attgcatggc agaggacatg acattcctgc ccaagcgcga cttgctgaca
     1021 ctcgaggagc tggaccggct gtgcgaggcg ttcatccggc tgggcacgcg gaagatccgc
     1081 attaccggcg gggagccgct ggttcgtcgc gacgtgatgc ggttgttcgg caggctgggc
     1141 gaccggcttg ggcatggctt ggatgagctg acggtcacca ccaacggctc gcaactggtg
     1201 aagcacgcgg aaaccctggt ggcggcgggc gtcaagcgga tcaacgtgtc gctggacacg
     1261 ctggatccgg tgcggttcgc ccagattacc cgctggggca agatcgagca gacgctggac
     1321 gggatattcg ccgccaaggc cgccgggttg gcggtgaaaa tcaatgccgt ggcgatgaag
     1381 ggcgtgaacg aggcggaatt cgacggcatg ctggcctggt gcggcgagca tgggttcgac
     1441 ctgtgcctga tcgagacgat gccgatgggc gacatctccg gcgatcggac ggatcagtat
     1501 ctgccgctgt cggtggtgcg ggcgcggctg cgcaagatct ggacgctgga ggataccgat
     1561 tacaagaccg gcggtccggc gcggtatttc accgtgaagg agaccggaca gcggatcggc
     1621 ttcattactc cgatgacaca taatttctgt gaaagctgca accgggtgcg gctgacctgc
     1681 accggcacgc tttatatgtg cctcggccag gatgattcgg cggacttccg tccgctgctg
     1741 cgggcggggg tatcggacga tcaactggat gatgcgatcc acgaagcgat ctcgcgcaag
     1801 ccgaaggggc acgatttcat cattgaccgg cggcacgaca agccggcggt ggcgcggcac
     1861 atgagcgtga cggggggtta ggagcaacag gcatccggca ggtcgcttgg gcagcctggc
     1921 gaatcatgcc ggacgcggtc cgcagacacg gagtaccgcc gcgaccttcc gcatgg
//
LOCUS       DASWQN010000221         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1078529, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000221 DASWQN010000000
VERSION     DASWQN010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078529"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VGC82_03610"
     CDS             complement(<1..198)
                     /locus_tag="VGC82_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422391.1"
                     /translation="MDKIVVLIAANMVAEVCSTYVLRVDNTLELYATEGLNRDAVHRT
                     VLSAHEGLVGLVASEATPLNLS"
     gene            complement(547..1800)
                     /locus_tag="VGC82_03615"
     CDS             complement(547..1800)
                     /locus_tag="VGC82_03615"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY0422392.1"
                     /translation="MGRLVMKFGGTSVANIDRIRNVARHVKREVDAGHDVAVVVSAMA
                     GKTNELVEWCGEASPMHDAREYDAVVASGEQVTAGLLAIALQAIGIQARSWQGWQIPI
                     RTSDAHASARILEIDGSELINRFSERKEVAVIAGFQGINPQTGRVTTLGRGGSDTSAV
                     AIAAAIHADRCDIYTDVDGVYTTDPRVVPKARRLDKIAFEDMLELASQGAKVLQVRSV
                     ELGMVHNMPVFVRSSFDRPEDIDPHGTPPGTLICSEEEIMESHVVTGIAFSKDEAQIS
                     VRQIQDKPGVAAAIFGPLAEANINVDMIVQNVSEDGATTDLTFTVPASDYVRARDTIT
                     GAKAKIGYVRLDSATDVAKVSVIGSGMRSHAGVAAKAFGALAARNINIRAITTSEIKF
                     SVLIDAAYTELAVRTLHTLYGLDQV"
     gene            1978..>2352
                     /locus_tag="VGC82_03620"
     CDS             1978..>2352
                     /locus_tag="VGC82_03620"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY0422393.1"
                     /translation="MGRYIDDILQPGEKVLYSTNAHWIFYVPAIAAWIVAILLFLLSR
                     QTDTEGIVLLCLSAAAVVAIVALYWTVKAWFHRWTTETDVTNLRVVHKTGFIKRRTFE
                     MSLDKVESVDVNQSILGRILNYG"
BASE COUNT          447 a          720 c          735 g          450 t
ORIGIN      
        1 gctgagattg agcggggtgg cctcgctcgc caccaggccg acgaggccct catgcgcgct
       61 caacacggtc cgatggaccg cgtcgcgatt gaggccttcg gtggcgtaga gttccagggt
      121 gttatcgacg cgcagcacat aggtcgagca gacctcggcc accatattgg cagcgatcag
      181 caccacgatc ttgtccaatc gttcctgggc gctgacttgc tccgccatgg tttcgcggag
      241 ccgtctcagc aagacgcggg ggcctcccga cgcgctccgc atgtgctggt atcctccccc
      301 gcaaatccag caggccgggt ggccggagct ggcgttaagc tcaagaaaaa caacaatttt
      361 atctattcgg cctcgccgca accctggaaa ccggccgaat caggacagcc aaaactgtgg
      421 cacagtttgc ggctgcccac ccatcaggcc gtatagcgaa ttgaggcttg ttttgccaag
      481 caaaacccct gccagaggcg taacgtctct gtcagcgcaa tgtgcggccg ctaacggaaa
      541 attcttctaa acctgatcga gtccatagag cgtgtgcagc gtgcgcaccg cgagttcggt
      601 ataggcggcg tcgatcagca ccgaaaactt gatttcggag gtggtaatgg cgcggatatt
      661 gatattgcgg gcagcaagcg cgccaaacgc tttcgcagcg acgccggcat ggctgcgcat
      721 gccgctgccg atgaccgaga ctttcgccac gtcggtggcg ctgtcgagcc gcacatagcc
      781 gatcttggcc ttggcaccgg taatcgtatc gcgggcgcgg acataatccg aggccgggac
      841 ggtgaaggtg aggtctgtgg tggcgccgtc ctcggagacg ttctgcacga tcatgtcgac
      901 attgatattg gcttcggcga gcgggccgaa gatcgcggcg gcgacgcccg gcttgtcctg
      961 gatctggcga acggaaatct gggcttcgtc cttggaaaag gcgatgccgg tgacaacgtg
     1021 gctttccatg atttcctcct cgctgcagat cagcgtgccc ggcggcgtgc cgtgcgggtc
     1081 gatatcctca ggtctgtcga aactggaacg tacgaacacc ggcatgttgt gcaccatgcc
     1141 tagttccacc gagcggacct gcagcacctt ggcgccctgc gaggccagtt ccagcatgtc
     1201 ctcgaatgcg atcttgtcga gccgccgcgc cttcggaacc acgcgcgggt cggtagtata
     1261 gacgccgtcg acgtcggtgt agatatcgca gcggtcggca tgaatggcgg cggcgatcgc
     1321 aaccgccgag gtgtcggaac cgccgcgccc gagcgtggtg acccggccgg tctgcggatt
     1381 gatgccctgg aagccggcga tgaccgccac ttccttgcgc tcgctgaaac ggttgatcag
     1441 ctccgagccg tcgatctcga gaatccgcgc cgaggcgtgg gcgtcgctgg tccgaatcgg
     1501 gatctgccag ccctgccagg agcgcgcctg aatgccgatc gcctgcagcg cgatcgcgag
     1561 cagcccggcg gtgacctgtt cgccggaggc caccacggcg tcatattcgc gcgcgtcatg
     1621 catcggcgag gcctcaccgc accattccac cagctcattg gtcttgcccg ccatcgcaga
     1681 caccaccacg gcgacgtcat ggccggcatc gacctcgcgc ttcacatgcc tcgcgacatt
     1741 gcggatacgg tcgatattgg cgaccgatgt gccgccgaat ttcatcacga ggcggcccat
     1801 gacgactggt gcattccctg acaggttaag taatgtgacc cgcaccgaaa cgggaagcgc
     1861 gcgggccaaa aggcgcgtat acatagcggc gcggttgggg gcaagcaacc ggcttccggc
     1921 taaccccccg agcgttacgg gacactgtgt ggtggtgcgg taattaaggc tgaacgtatg
     1981 gggcgttata tcgacgatat cctgcagccc ggcgagaagg tgctgtattc gaccaacgcg
     2041 cactggatat tttatgtgcc ggcgattgcg gcctggattg tggccatctt gctgttcttg
     2101 ctgtcgcgac agaccgatac cgaaggaatc gtgctgttat gcctgtcggc cgcggccgtg
     2161 gtggcgatcg tggcactgta ctggaccgtg aaagcctggt tccaccgctg gaccaccgaa
     2221 accgacgtca ccaacctgcg cgtcgtccac aagaccggct tcatcaagcg acgcacattt
     2281 gaaatgagcc ttgacaaggt cgagagcgtg gacgtcaacc agagtattct cggccgcatc
     2341 ctcaactacg gc
//
LOCUS       DASWQN010000222         6730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_418985, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000222 DASWQN010000000
VERSION     DASWQN010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418985"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..537
                     /locus_tag="VGC82_03625"
     CDS             256..537
                     /locus_tag="VGC82_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422394.1"
                     /translation="MAMSQSEDDVGRDVASGAAPRYSRRLSDKILIAFHHACDQADFE
                     VAEQLLHILEMMLTRRPLTPDGTRRRNMESLVAAHERLWHLRHPTSDEI"
     gene            complement(577..1338)
                     /locus_tag="VGC82_03630"
     CDS             complement(577..1338)
                     /locus_tag="VGC82_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0422395.1"
                     /translation="MVTTEGWLLGGRVRYRQPAIGFRSGIEPVLLAAAVSARGGEHVL
                     EAGTGAGAALLCLTSRVPGVVATGIEADAAMAGLAAENARDNGFTGIEIITGRVETAA
                     LPGLFDHAIANPPYHQAGGSASPVAGRETAKRGSETLIPVWVGRLGAALRGRGSLTLI
                     VPAGMVASCLAAMTASRCPCTSVFPLWPKAGRPAKLVLLRGTKDARTPMRLLAGMVLH
                     QADGAFTEAAKRLLEDAAALPLERQAGVDSDQAGD"
     gene            complement(1332..1538)
                     /locus_tag="VGC82_03635"
     CDS             complement(1332..1538)
                     /locus_tag="VGC82_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY0422396.1"
                     /translation="MRVVVTSNNPVRLSFLTALLADAGIEAVLLDSHTSVLEGSAGAI
                     PRRLVVSTENFARACRLLRDAGEW"
     gene            1827..2822
                     /locus_tag="VGC82_03640"
     CDS             1827..2822
                     /locus_tag="VGC82_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0422397.1"
                     /translation="MNEDALSVLVSLLGADLEACNNVIVQRMDSPVALIPQLAAHIVA
                     AGGKRLRPLLTLASARLCGYPGPEGGARHVDLAACVEFIHTATLLHDDVVDESQLRRG
                     LASANAVFGNKPSVLVGDFLFARAFQLMVDDGSLRVLAILSKAAATIAEGEVLQIVTQ
                     NDLSTTEARYLEVVQGKTAALFAAACQVGAVVANRPEREEAALAEYGMKLGIAFQLVD
                     DALDYVADQATLGKTIGDDFREGKITLPVLVAFLAGNDLEKAFWRRTIEALKQTDSDL
                     DHAIALIEGHGAIKATLERALSFVQEAKAALTVFADGPFRDALAGVADYTVQRLR"
     gene            3004..5103
                     /gene="rnr"
                     /locus_tag="VGC82_03645"
     CDS             3004..5103
                     /gene="rnr"
                     /locus_tag="VGC82_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY0422398.1"
                     /translation="MAKRIKHRGKPVSEPRVRPPYRPSSRGAAGNLPETAVVQVTGTD
                     RDGDALARPVEWDRGSGQPPLIHMHPERQGSAALAPGERVLVRLTPMGPGRYEARTLR
                     RIGENPGRILGVFKRGRGENRITPTDRRSKAEWVVPPGQEAGAEADEIVLAEPLAQGP
                     QLGLKPARVLERLGKLGDARSISLIAIHTHDIPQTFPEAALDEAERARGVRLGSRTDL
                     RDLKLITIDGADARDFDDAVFAEPDPDQAGGFRLVVAIADVAHYVRPGSALDQAAGIR
                     GNSVYFPDRVVPMLPEALSNGWCSLRPREDRGCLFVEMRIDQAGRKTSHRFGRGLMRS
                     AARMTYEEVQARFDAEPDASAGDARDDLRLPLVPLYAAYRALLAARVERGTLDLDLPE
                     RTVVLDDQGRVTSVAPRPRLDSHRLIEEFMVLANVAAAEELERLHTPCMYRVHAPPSD
                     EKLDALREFLATLGISMPAGNQVHPRDFDRVLRRYAGTPESQLISEVMLRSQSQAAYS
                     PDNIGHFGLALPRYAHFTSPIRRYADLLVHRALIKGLRLGRDGLSGDQVSVLPEIGER
                     ITELERRAQQAERDAIDRYLTAFMAGRVGGRFAARISGVTRFGLFVTVTETGATGIVP
                     FASLPDDYWQYDDREQTLTGRRTRKIYRLSQEVDVLLSEANPVTGGMVFHVLESDPPK
                     SSGRRGKAAPEKRPRRR"
     gene            5103..6617
                     /locus_tag="VGC82_03650"
     CDS             5103..6617
                     /locus_tag="VGC82_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0422399.1"
                     /translation="MRIGVLLVLFVIGHANAQGLAGAEFDQDRLTAVYTEALTFIAPR
                     IIDPVSVPSLAVWGLQGLTALDPELRIVATEVRLKLLRRTEIVLDLAAPKDESPASWA
                     KVAAALTAASLPVSVPIRRAGTQGIIQCFFDQMFSRIDPYSRYVPPVEAAEDRAKRAG
                     RAGLGLTVGQRGTLIEVRTVVRDSPASIVGIRPGDILVSIDGQRTVDQDPATIAALLA
                     GREGAAVTLSWRGRDGRTRDAELVRVMVPPETVFAQRFADVIVLRVTSFSNSTASHLA
                     LSVQDALAQPHPVDGIVLDLRDNRGGLLRQSVTAADTFLPAGLVATTAGRDPDSMKIW
                     RSTEGELGENVPLVILVDGRTASAAEILAAALADRGRAVVVGSATVGKGMVQTIDPLP
                     DGGELFVTWSQTVAPLGWPLQGLGVLPQVCTSLGQDALNRQLASLSTGFQPMAAAIRR
                     SRAARIPVSATEALEIRAACPASEGRATDLIAARMLIANPAAYAAALLSPMPDR"
BASE COUNT         1132 a         2184 c         2237 g         1177 t
ORIGIN      
        1 gtattctagg gtatatatgt tcagtctgat ggtttttgta tcacacaacc tagctatttt
       61 cgtttcggcg gcatttttga cggagctggc cttggaatta acgagttttt cacgttcaag
      121 atgtttaatt gtggtgaatt catatcgtgc cctgatccac tcaggcgctg gtcgggatca
      181 tgatgttcgt gcgggaaagg tgcatattgg caccggttgg aggctgacat ggctaccgtt
      241 accgtcgtgc cagggatggc catgagccaa tctgaagacg atgtcggccg ggatgtagca
      301 tccggtgcgg ccccgcggta cagccggcgc ctgtcggaca aaatcctgat cgcgttccat
      361 catgcctgcg accaggcgga cttcgaggtc gccgaacagc ttctccatat cctggagatg
      421 atgctgaccc gccgcccctt gacccccgat gggacgcgcc ggcgcaacat ggaaagcctc
      481 gtcgcggcgc atgagcggct atggcacctg cgccatccaa cctcggacga gatttaatcg
      541 cccgcctggt ttaatcgccc gcccggcccg cgtggcttaa tcgccggcct ggtctgaatc
      601 gacgccggcc tgtctctcca gaggcagcgc tgcggcatct tccagaagac gcttggcggc
      661 ctcggtaaac gcgccgtcgg cctggtgcag caccatccct gccagcaacc gcatcggggt
      721 gcgggcgtcc ttggtgccgc gaagcagcac cagcttggcc ggccgtccgg ctttcggcca
      781 aagcggaaaa actgacgtgc acgggcagcg cgatgcggtc atcgccgcca ggcaggacgc
      841 gaccatccca gccggcacga tcagcgtcag gctaccccgg ccgcgcagcg cggcaccaag
      901 ccgcccgacc cagacgggga tcaaggtttc ggagccgcgc ttggcggttt ccctcccggc
      961 caccggcgaa gcggaaccgc cagcctgatg atacggtgga ttggcgatcg catgatcgaa
     1021 caggccgggc agggctgcgg tctcgacgcg gccggtgatg atctcgatgc cagtgaagcc
     1081 attgtccctg gcattctccg ccgccaatcc ggccatcgcg gcatcggcct cgatgccggt
     1141 ggcgacgacg ccgggaaccc gggatgtcag gcaaagcaac gccgcgccgg caccggtgcc
     1201 tgcctccagc acatgttcac caccgcgagc ggagaccgcc gcggccagca gcaccggttc
     1261 gatgccgctg cgaaagccga tggccggctg gcggtacctg acccggccac ccagcaacca
     1321 accctctgtc gtcaccactc gcccgcgtcc cgcagcagcc ggcaggctcg tgcaaaattc
     1381 tcggtgctga cgaccagccg ccgcgggatc gccccggcgc ttccttccag cacgctggtg
     1441 tggctatcga gcagcacggc ctcaatgccg gcatccgcca gaagggcagt cagaaatgac
     1501 agcctgaccg gattgttgga ggtgacgacg acgcgcatgc gtggatcgtg agccggatgg
     1561 tgcctcggcg caacgggaca tgttagcctc cgaaccgggc gctgggcggg tgcgtcggtg
     1621 gcaaaacccg cggatttgct gcgaccgctg gcccggcggt cgccgaaacg catggatgaa
     1681 cggtaacgct gttcggttgt ggcttgaccc ttgttggtag ccaccgatat gaaggcagcc
     1741 ctagtgcaag gtgggagaac cagttgggcg gcgacgccag tcacctcgat ctaggcgtgg
     1801 cgcttgtgtc cgatccggcg ccacacatca acgaggacgc attatcggtc ttggtcagcc
     1861 tgctcggcgc ggacctggag gcctgtaaca acgtcatcgt ccagcgcatg gacagtcctg
     1921 tggcgctgat cccgcagctc gcggcccaca tcgttgctgc gggcggcaag cggctgcgtc
     1981 ccctgctgac actcgcctcg gcccggctgt gcggctaccc cgggccggag ggcggcgccc
     2041 gtcatgtgga ccttgccgcc tgcgtcgagt tcatccacac cgcgacgctg ctgcacgacg
     2101 atgtggtgga tgaaagccag cttcgccgcg gcctggcgtc cgccaatgcg gtgttcggca
     2161 ataagccatc cgtgctggtc ggggatttcc tgttcgcccg cgccttccaa ctgatggtgg
     2221 acgatggctc gctgcgcgtg ctggcgatcc tgtcgaaagc cgccgcgacc atcgcggaag
     2281 gcgaggtact gcagatcgtc acgcagaacg acctgtccac caccgaagcc cgctacttgg
     2341 aagtcgttca aggcaagacc gccgcgctat tcgccgccgc ctgccaggtt ggcgcggtcg
     2401 tcgcaaaccg tcccgaacgc gaggaagcgg cccttgccga atacggcatg aagctgggca
     2461 tcgcctttca actggtggac gatgcgctgg attacgttgc cgaccaggcc accctgggca
     2521 agacgatcgg cgacgatttt cgcgaaggca agatcacctt gccggtcctg gtcgccttcc
     2581 ttgccggcaa tgaccttgaa aaggcgtttt ggcgccgcac catcgaagcc ttgaaacaga
     2641 ccgattccga tctcgaccat gccattgcct tgatcgaagg ccatggggcc atcaaggcta
     2701 ccctggagcg tgcgctaagc ttcgtgcagg aggcgaaagc cgccctgacg gtgtttgccg
     2761 acggcccatt ccgcgacgca ctcgccggtg tcgccgacta caccgtgcaa cggctgcgtt
     2821 gaagttcgat gtcgcaaggc cacctgtccg ggggccacga ccgctgcctg gaataaaact
     2881 gtgtcgtttc gggcgcagcc gatagccgac atcagcggca ccctcgaacg actcaagctg
     2941 gcatggaact gtccaccggc ctgtggcaaa gtgccgcgag cataatcgta gtcccggaaa
     3001 tcagtggcga aacgaattaa gcatcgaggc aaacccgtct ccgaaccccg cgtccggccc
     3061 ccttatcggc catcgtctcg cggtgccgcc ggcaacctgc ctgaaacggc ggtggtccag
     3121 gttaccggca ccgatcggga tggcgatgcg ctggccaggc cggtggaatg ggatcgagga
     3181 tcaggtcagc cgccattgat ccatatgcac cccgaacgtc aaggttcggc cgcgctggcc
     3241 ccgggcgagc gggtgctggt ccggctaacg cccatgggac cgggccgcta cgaagcgcgc
     3301 acgttgcgcc ggatcggcga aaaccctggc cgcatcctcg gggttttcaa acgcgggcgt
     3361 ggtgaaaacc ggatcacgcc gaccgatcgc cggtccaagg ccgaatgggt cgtcccgccg
     3421 gggcaggagg caggcgccga ggcggacgaa atcgtgctgg cggaaccgct cgcccagggt
     3481 ccgcagctcg gcctgaagcc ggcacgggtg ctggaacgac tgggcaagtt gggcgatgcc
     3541 cgctcgatca gcctgatcgc catccacacc cacgatatcc cgcaaacctt cccggaggcg
     3601 gcattggatg aggcggaacg cgcccgcggc gttcggcttg gcagccgcac cgacctgcgc
     3661 gacctgaagc tgatcaccat cgacggcgcc gacgcccgcg acttcgacga cgcggtattc
     3721 gccgaaccgg acccggacca ggcgggcggt tttcggctgg tggtcgccat cgccgatgtg
     3781 gcgcattacg tccgccccgg atcggcgctg gatcaggccg ccggcattcg cggcaacagc
     3841 gtctattttc ccgaccgcgt cgtgccgatg ctgcctgaag cgctgtcgaa tggctggtgc
     3901 agcctgcgcc cgcgggaaga tcgcggctgc ctgttcgtcg agatgcggat cgaccaggcc
     3961 ggccgcaaga ccagccatcg cttcggccgg ggactgatgc gcagcgccgc ccgcatgacc
     4021 tatgaggagg tccaggcccg attcgacgcg gaaccagatg cgtccgccgg ggatgcccgt
     4081 gacgacctcc gcttgccgct ggtgcctttg tatgccgcct atcgggcgct gctggctgcc
     4141 cgggtggagc gggggacgct cgatctcgat ctgccggaaa gaacggttgt actcgacgac
     4201 cagggtcgcg tgaccagtgt cgcaccccgg ccgcggctgg acagccaccg gctgatcgag
     4261 gagttcatgg tgctggccaa tgtcgccgcg gccgaggaac tcgaacgcct gcacacgccg
     4321 tgcatgtacc gggtgcatgc gcccccgtcg gatgagaaac tcgacgcact acgggagttt
     4381 ctcgcgacct tggggatctc gatgccggcg ggcaaccagg tgcacccgcg cgacttcgac
     4441 cgcgtcctgc gccgttacgc cggcacgccg gagtcgcaac tgatcagcga ggtgatgttg
     4501 cgcagccagt cgcaggctgc ctacagcccg gataatattg gccatttcgg tcttgcactg
     4561 ccgcgctatg cgcacttcac cagtccgatc cggcgctatg ccgacctgct ggtgcatcgc
     4621 gcgctgatca agggtctgcg tctcggccgc gatggccttt ccggcgatca ggtctcggtc
     4681 cttccggaaa tcggcgagcg tatcacggag cttgagaggc gggcgcagca ggctgaacgc
     4741 gacgccatcg atcgttatct gaccgccttc atggccggtc gggtcggcgg acgtttcgcc
     4801 gctcggatat cgggggtgac ccggttcggc ctgttcgtta ctgtgacgga aaccggtgcc
     4861 accggcatcg tgccgttcgc gagcctgccg gacgactact ggcaatatga cgatcgagag
     4921 caaaccctga ccggccggcg cacgcggaag atctatcgcc tgtcgcagga agtggatgtc
     4981 ttgctgtccg aggccaatcc ggtcaccggc ggcatggtgt tccatgtgct cgaaagcgac
     5041 ccgcccaaga gcagcgggcg cagggggaag gctgcgccgg agaaacggcc tcgccgccgg
     5101 tgatgcggat cggcgttctt ctggtgctgt tcgtgattgg acacgccaac gcccaggggc
     5161 tggcgggcgc cgaattcgac caggaccgcc tgaccgcggt ctataccgag gcactaactt
     5221 tcatcgcgcc gcgaatcatc gacccggttt cggtaccgag cctggcggtt tggggtttgc
     5281 agggtttgac cgccttggac cccgaactgc ggatcgtcgc taccgaggtt cggctgaagc
     5341 ttttgcgccg aactgaaatc gtgctcgatc tggccgcccc caaggatgag tctccggcat
     5401 cctgggcgaa agttgccgcc gcgctgacgg ccgccagtct gccggtctcg gtgccgatca
     5461 ggcgggcggg cacccaaggc atcatccaat gctttttcga ccagatgttc agccgtatcg
     5521 acccctactc ccgatacgtg ccgccggtcg aggccgctga agaccgggcg aaacgggctg
     5581 ggcgcgcggg cctggggctt acggtgggtc agcggggcac gctgatcgag gtcaggacgg
     5641 tcgtgcggga cagcccggct tccatcgtcg ggatcaggcc gggcgatatc ctggtcagca
     5701 tcgatggcca gcggacggtg gatcaggatc cggccacgat cgccgccctg ctggccggcc
     5761 gcgagggggc cgcggttacc ctttcctggc gcggacggga cggccgaacc cgcgatgccg
     5821 agttggttcg ggtgatggtg ccgccggaaa ccgtctttgc ccagcgtttc gccgacgtca
     5881 tcgtcttgcg cgtcaccagc ttcagcaaca gcacagcttc gcaccttgcg ctgtcggtgc
     5941 aggatgcgct ggcgcaaccg catcccgtcg atggcatcgt gctggatctg cgcgacaacc
     6001 gcggcgggct gctgcgccag tcggtgaccg cggcggacac gttcctgccg gcgggtctgg
     6061 tcgcgaccac ggccggacgc gatccggact cgatgaagat ctggcgctcc accgaaggcg
     6121 agctgggcga aaacgtcccg cttgtcatcc tggtcgatgg ccgcaccgcg agtgccgcgg
     6181 aaatcctcgc cgccgccttg gcggaccggg gaagggcggt ggtcgtgggc agcgcgacgg
     6241 tggggaaggg gatggtgcag acgatcgatc cgttacccga cggcggcgag ctgttcgtta
     6301 cctggagcca gacggttgcg ccgcttggct ggccgctgca gggcctgggg gtgctgccgc
     6361 aggtgtgcac cagcctgggc caggatgccc tgaaccggca gcttgcgtca ttgtccaccg
     6421 gcttccagcc catggccgcc gccattcgcc ggtcccgtgc tgcccgcatt ccggtgtcgg
     6481 cgaccgaggc gctggaaatc cgagcggcct gtccggcatc ggagggccgc gccaccgatt
     6541 tgatagcggc gcggatgctg atcgccaacc cggcggcgta tgcggcggca ctgctttcgc
     6601 cgatgccgga ccggtagagc tggaaagacg tgtgtcccgg cggggtagtc cctcactttg
     6661 aaaatatcaa gaattagacg gcttggcgaa tggtccggct gcctgcgata tgcttagcga
     6721 taggcatagg
//
LOCUS       DASWQN010000223         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_155340, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000223 DASWQN010000000
VERSION     DASWQN010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155340"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..966)
                     /locus_tag="VGC82_03655"
     CDS             complement(112..966)
                     /locus_tag="VGC82_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0422400.1"
                     /translation="MPVKNIRGVDIVYEILGDSGPWVTVTPGGRRGLTGERALAALIA
                     EAGYRVLVHDRRNTGASGIGFPGDSESREQAEDLLVLLGELGTGPAYVAGSSSGARMS
                     ILLAQHHPREVKGLLLWRVTGGEFAAKRLAFNYYEQYIDAVTKGGIDAVTKGGIDAVL
                     ASEHFAAMAEANPVNVETLRRMGGDAFKASMQRWLAGFRAGCDYPVAGISPENMRRIT
                     VPAIVVPGNDYTHPPAAAQAAHRLLRNSVYQEVLTQQVNADVDLVGWESGTGTLAARF
                     IDFMRGQA"
     gene            1136..>2311
                     /locus_tag="VGC82_03660"
     CDS             1136..>2311
                     /locus_tag="VGC82_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0422401.1"
                     /translation="MRPEDVIADKRRPYTGAEYIQSLRDGREVYIDGERVADVTTHPA
                     FRNSVRSIARLYDALHDPKRRDTLTCATDTGSGGYTHKYFRVPRSREDMIGAQGAIAE
                     WSRLTYGWMGRTPDYKAAFSNTLGANAEYYGPYAGNALAWYRRAQETMPFMNHAIVNP
                     PIDRHKPAEETKDVFVCVQKEVDGGIIVSGAKVVATSAAMTHYNFMGQSSKTETEDLD
                     MSLMFLVPIDAPGLKLFCRTSYEQAAQSRGPFDYPLSSRFDENDAIFVMDKVFIPWED
                     VFIYRDPARVLSFFGRSGFIDGAQFQGCTRFAVKLDFIVGLLAKALRCTGGDEARGNQ
                     VLLGEAVAWRNLFWSLSNAMAHNPTPWNGDAVLPDRKAGQAYRVFAPDAYPRIKDIIE
                     "
BASE COUNT          435 a          758 c          694 g          424 t
ORIGIN      
        1 gtcagcagcg aggcggactc ccttctccat gcttccatga tctccgcgtc ctccgcgtca
       61 aaagccttga ccaacctctt cgctctggcc tcaaggagga aaaaactgga actatgcctg
      121 cccccgcatg aagtcgataa accgggcggc gagggttccg gtgccgctct cccagccgac
      181 aagatcgacg tcggcattca cttgttgcgt caacacctcc tgatagacgc tgttgcgcaa
      241 caaccggtgc gccgcctggg ccgcggccgg cggatgcgta tagtcgttgc ccggaacgac
      301 gatcgccggc acggtgatcc gccgcatatt ctcggggctg atccccgcca ccggatagtc
      361 gcagccggct cggaatccgg ccagccagcg ctgcatcgaa gccttgaatg cgtcaccgcc
      421 catccgccgc aaggtttcaa cgttcaccgg attggcctct gccatagccg cgaaatgttc
      481 gctggccagc accgcgtcga tgccgccttt cgtcaccgca tcgatgccgc ctttcgtcac
      541 cgcatcgatg tattgctcgt agtagttgaa cgcaaggcgt ttcgcggcaa attccccgcc
      601 ggtcacccgc cacagcagca gccccttcac ctctcgcgga tgatgctgcg ccagcaggat
      661 cgacatgcgg gcgccggagg acgaaccggc gacataggcc gggccggtgc cgagttcgcc
      721 gagtagaacc agcaggtcct cggcttgctc acgactttcg ctgtctccgg gaaagccgat
      781 gcccgaagcg ccggtgttgc gccggtcatg gaccagcacg cgatatccgg cctcggcaat
      841 cagcgccgcc agtgcgcgtt caccagtcag gccacgacgg ccgcccggcg tcacggtgac
      901 ccaggggccg ctgtcgccca ggatctcata gacgatatcg acgccacgga tgttcttgac
      961 cggcatgtct gacgccctcc ccatcggcat gaacgacggc cggaaggttc ggcacggcgg
     1021 acgctggctg tcaatcggca gccctgacat gtaggttcgt ccgcagcgca ccgtggtgca
     1081 ccgatggtgc gaccccggcc gcacgggcta tggtcggtca accaggagaa gccgcatgcg
     1141 tccggaagat gtcatcgccg acaagcgccg gccctacacc ggggcggaat acatccaaag
     1201 cctgcgcgat ggccgcgaag tgtatatcga tggcgaacgc gtcgcggatg tcacgaccca
     1261 tccggcgttc cgcaactccg tccgctcgat cgcccgcctg tatgacgcgc tgcatgaccc
     1321 gaagcggcgc gacacgctga cctgcgcgac cgacaccggc tccggtgggt acactcataa
     1381 atacttccgg gtgccacgtt cgcgcgagga tatgatcggt gcgcaggggg cgatcgccga
     1441 atggtcccgg ctgacctatg gttggatggg ccgcaccccg gactacaagg ctgccttcag
     1501 caatactctg ggcgccaacg ccgaatacta cggtccctac gccggcaacg ctcttgcgtg
     1561 gtatcgccga gcgcaggaga ccatgccgtt catgaaccat gcgatcgtca atcctccgat
     1621 cgatcggcac aagccggcgg aggaaaccaa ggatgttttt gtttgcgtgc aaaaggaagt
     1681 ggacggcggc atcatcgtct ccggcgccaa ggttgttgcc acatctgcgg cgatgacgca
     1741 ctacaacttc atgggccagt cctcgaaaac cgagaccgag gatctggaca tgtcgctcat
     1801 gttcctggtg ccaatcgatg cgccgggact gaaactgttc tgccgcacgt cgtacgaaca
     1861 agccgcccag tcgcgcggac cattcgacta tccgctgtct tcgagattcg acgaaaacga
     1921 tgctatcttc gtcatggaca aagtattcat cccgtgggag gacgtgttca tctatcgcga
     1981 tcctgctcgt gtattgagtt tcttcgggcg ctccggcttc atcgacggcg cgcagttcca
     2041 gggctgcaca aggttcgcgg tcaagctgga ttttattgtc ggactgctgg caaaggcgct
     2101 gcgctgcacc ggcggggacg aggcgcgcgg aaaccaggtg ctgctggggg aagccgtcgc
     2161 ctggcgcaat ctgttctggt cgctgtccaa cgccatggcg cataacccca ctccgtggaa
     2221 cggcgacgcc gtactgccgg accgcaaagc cggtcaggcc tatcgcgtgt tcgcgccgga
     2281 tgcctatccc aggatcaagg acatcattga g
//
LOCUS       DASWQN010000224         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2530455, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000224 DASWQN010000000
VERSION     DASWQN010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2530455"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VGC82_03665"
     CDS             complement(<1..332)
                     /locus_tag="VGC82_03665"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEY0422402.1"
                     /translation="MDHASVCTLDCPDTCSLTVTVEDQRITKVRGSHALSYTSGVICN
                     KVAHHTAEFVHGPGRILHPLQRVGPRGSGTFRRIGWEEALDVIHHRVGAVLDRWGPQA
                     VMPLNYAGP"
     gene            complement(510..1337)
                     /locus_tag="VGC82_03670"
     CDS             complement(510..1337)
                     /locus_tag="VGC82_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0422403.1"
                     /translation="MIDPTTDRVLITGAAGAIGTAVRDGLRANWRRLRLTDIRPVQNV
                     TDNEEAIIADIADRSAVEAMMRDVRAVVHLAGVLGNYDLEALFRINARGVFDVFEAAR
                     LAGVERIVFASSNHAFGCYPITVPVSPELPPRPDSLYGVFKVLGETMLRNYYDRHGIS
                     SVSLRIGTYRSLPIDQRSLATWLSPADVAQLVDLSLRHPNPGCMVVNGYSNNTRLKTH
                     DPNWAVLGYQPRDNAEDYREMLRSQGVDVDAPDRDEWEWPEHGGSHARAPERPARHT"
     gene            complement(1411..2574)
                     /locus_tag="VGC82_03675"
     CDS             complement(1411..2574)
                     /locus_tag="VGC82_03675"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0422404.1"
                     /translation="MPAHRTGEHFLLAAQALTGQINDARNEIERTRLMPATLIAALRA
                     TGIFRMWLPQALGGPQLSVAEFVRVVEALSYVEGSVGWCTGLGAAYSRFAGYLPDAIA
                     TQIFEDNIMAGTLAPHGRGTKVPGGYRVTGRWSWGSGIMHSQWLIVAFLTQAGNGPIM
                     VGGQPETRIGFLPIAGNVEVIDTWNVGGLRGTGSHDFAISDVFIPDDFTLDGVDPPPR
                     HAGSLYAISLNSVYPFVIAAVPLGITMAAFDAFLSLAKIKTPISGSVLLRDKPTVQSM
                     IGRSHALLHSAREFYYATARSMPEWAETAIPLTLDQRAQVRLAVAQISEVCKQVVRSL
                     YDAAGGSSVYETCPLERHFRDIHAAAQHVQVSPNNFEFAGRVVLGLDAGTARF"
     gene            complement(2523..>2749)
                     /locus_tag="VGC82_03680"
     CDS             complement(2523..>2749)
                     /locus_tag="VGC82_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422405.1"
                     /translation="KLRETTVSRITYTEATASARARHISVRPVAEFALATSRPMANIS
                     LPLARFGLRHEVKPCRHIEQASIFSWLLKR"
BASE COUNT          536 a          851 c          861 g          501 t
ORIGIN      
        1 ggcccggcgt agttcagcgg catgaccgct tgcggacccc aacgatccag caccgcgccg
       61 acgcggtgat ggatgacatc gagcgcctcc tcccacccga tacggcgaaa ggttcccgaa
      121 ccgcgcggac cgacgcgctg cagcggatgc aggatacggc cggggccatg cacgaactca
      181 gcggtgtgat gagccacctt gttgcagatc acccccgaag tataactcag cgcatgtgaa
      241 ccgcgcacct tggtgatccg ctgatcttca accgtcaccg tcagactgca agtgtccggg
      301 cagtcaagcg tgcaaacgct ggcatgatcc atgatctact ccgggtgtat ggtccgcaac
      361 ttcggcatta ctgacgtgaa cgcgcttccc gccagcttga acaggctgta gcacatcgcg
      421 gcgggaaacc catgcacgtg ctgcatttgg tctgcatctt tatcggacga ttggccacgg
      481 cgaagcggct tcccggcctg acgtgccgat caggtatggc gggccggccg ctccggcgct
      541 cgggcgtggg agccgccgtg ttccggccac tcccattcgt cgcgatccgg cgcatcgacg
      601 tcgactccct ggctgcgcag catctcccga tagtcttcgg cattgtcacg cggctggtag
      661 cccagcacag cccaatttgg atcgtgggtc ttcaggcgcg tgttgttgga gtagccgttg
      721 accaccatgc aaccgggatt cgggtggcgc agcgacaggt ccacgagttg cgcgacatcg
      781 gcggggctga gccaagtcgc gagagagcgt tggtcgatcg gcagcgagcg gtaggtgccg
      841 atgcgcagcg acactgagct gataccgtgg cggtcgtagt aattgcgcag cattgtctca
      901 cccaggacct tgaacacgcc gtacagacta tccgggcgcg gcggcaactc gggcgagacg
      961 ggcaccgtga tgggatagca gccaaaggcg tggttgctgc tggcgaagac gatgcgctcc
     1021 accccggcaa gccgcgctgc ttcgaacacg tcgaacacgc ctcgggcatt gattcggaac
     1081 agtgcctcca ggtcgtagtt gccaagcacg cccgccaaat gcacgacagc ccgcacatcc
     1141 cgcatcatgg cttcgacggc cgagcggtca gcgatgtcgg cgatgatcgc ctcctcgttg
     1201 tcggtcacgt tctgcacggg ccggatatcg gtcaggcgga ggcgacgcca gttcgcgcgc
     1261 agaccgtcgc gcaccgcggt gccgatggcg ccggcggccc cggtgatcag gacgcggtcg
     1321 gtcgttggat cgatcatgaa cgttctcctc tacggcaacc gtagcggaac acaggcggat
     1381 gagacaggcc agcaggatcg cgtcacgact tcagaacctg gccgttccgg cgtccagccc
     1441 gagtacaacc cgtccggcaa attcaaaatt gtttgggctg acctggacgt gctgtgcggc
     1501 ggcgtgaatg tcgcggaaat gccgctccag cgggcaggtt tcgtaaaccg acgagccacc
     1561 ggccgcgtcg tatagactac gaaccacttg cttgcagacc tcgctgatct gggcaacggc
     1621 aagtcgcacc tgggcacgct gatccagcgt caggggtatg gcggtttcag cccactccgg
     1681 catgctgcgg gcagtcgcgt agtagaactc acgggcactg tgcagtaagg cgtgagatcg
     1741 cccgatcatc gattgtactg taggcttgtc acgcagtaaa accgagccgc taatgggtgt
     1801 cttaattttg gcgaggctaa ggaacgcgtc gaacgccgcc attgttatgc cgagcggcac
     1861 cgccgcgatc acgaatggat agaccgaatt caacgagatc gcatacaggc tgccggcatg
     1921 tcgcggtggc ggatcgactc cgtccagcgt gaaatcatcg ggaatgaata cgtcgcttat
     1981 agcgaagtcg tgactgccgg tgccgcgcag gccaccgacg ttccaagtgt cgatgacctc
     2041 gacattcccg gctatcggga ggaatccgat acgcgtttcg ggctgtccgc cgaccattat
     2101 cgggccgttg ccggcctggg tgaggaatgc gacgatcagc cactgactgt gcatgatgcc
     2161 gctgccccac gaccaccggc cggtgacccg gtagccgccc gggaccttcg ttccgcgtcc
     2221 gtgcggcgcc agcgttccgg ccatgatatt gtcttcgaat atctgcgtcg cgatcgcgtc
     2281 cggcaggtat cccgcaaaac ggctgtatgc agcaccaagg cccgtgcacc agccgacaga
     2341 gccttctacg tacgacagcg cctcgaccac acgaacgaac tcggcgacgg aaagctgggg
     2401 accacccagg gcctgtggta gccacatccg gaagatgccg gtcgcccgca gcgccgcgat
     2461 cagcgtcgcg ggcatcaaac gcgtccgctc gatttcgttc cgcgcgtcgt ttatttgacc
     2521 tgtcagcgct tgagcagcca ggagaaaatg ctcgcctgtt ctatgtgccg gcatggtttc
     2581 acctcatgac gcaaaccaaa tcgagcgaga ggaagcgata tattagccat cgggcgggat
     2641 gtggccagag caaattcggc aacaggccga accgagatat gacgtgccct ggcggaagca
     2701 gtggcttcag tataggtgat tcgactcacg gtagtctcac gtaacttcg
//
LOCUS       DASWQN010000225         4073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1210949, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000225 DASWQN010000000
VERSION     DASWQN010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210949"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1457)
                     /locus_tag="VGC82_03685"
     CDS             complement(75..1457)
                     /locus_tag="VGC82_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEY0422406.1"
                     /translation="MLDQNIDCIAASPDCARPVHIVRPDRLAGFLDTLPSGQTAFLRD
                     TGFEAKGGELRFLPGPEGVAGAVLGIGTDTSPFVFGSLAMQLPDVCAWRLEQGDFDSE
                     AAVLGYCLGAYRYSHFVSPGRKPASLFVPPGHEASLSQAAATWMVRDLINTPANILGP
                     VELAEFTVALAKRYGAQSELSADAALEQAYPTIAAVGRGSVRPPRVATFHWRGSSAHE
                     ASPLLSLCGKGVCFDTGGYDLKPSAGMLRMKKDMGGAATVLGLARTIMEADLPLRLAV
                     RIGCVENSVSGTAMRPSDVIRTRSGLTVEVGNTDAEGRLVLCDLLHEASDENPSLLVD
                     CATLTGAARVALGPDIPALFSNDDSLAEAIMRFGNEVHDPLWRLPLWPGYQDWLKSPV
                     ADTNNVASKPLAGAIVAGLYLQRFVKAGIPWMHFDLYAWNDQSRPGRPEGGEAMAIRA
                     LFALAKERFC"
     gene            complement(1867..3687)
                     /gene="htpG"
                     /locus_tag="VGC82_03690"
     CDS             complement(1867..3687)
                     /gene="htpG"
                     /locus_tag="VGC82_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEY0422407.1"
                     /translation="MNDTVLEQHSFGAEVGRLLDLVVHSLYTEREIFLRELVANAADA
                     VDRRRFESLTDPTLAPPTDAKIRVVPDKDGSTLLISDDGIGMTRQELIDNLGTIARSG
                     TRAFAQNLATAKPEDRPSLIGQFGVGFYSAFMVADRVEVTSRKAGAEEAFTWASEGAG
                     AFTLAPATREQAGTDVVLHMKSDASEYLEPIRLETVIRKWADHITLPITVARDGKDEP
                     ANEGTALWRKAKSEVTEESYKEFYRHLGLFFDDPWATLHWRAEGTLEYFCLLFIPGMK
                     PFDAVDNDRDAHVRLHVRRMFITDKAELLPHWLRFVNGVVDTEDLPLNVSREMLQTTP
                     VLARIRKAMIGRVLSELKNRTKDAEDYAKFWGNFGPILKEGVWEDAEHRAEIASLLRF
                     KSSTQEGWTSLPDYVSRMQEGQDTIYYLTGDHADALKSSPQLEGFRSKGLEVLLMSDS
                     IDSFWPERLDSFEGKKLRSVTQAAEDLGKGEDQPDISPLLAAMKANLAGRVTDVRSTA
                     RLTDSAVVLASDGKGPDLAMQRLMRRAGRAMMPAKPVLEVNPRHKLIEALSAKLDDSG
                     LIAEASETLLDLAQVQEGDLPTDSSAFARRVTSLLASSLG"
     gene            3837..>4073
                     /locus_tag="VGC82_03695"
     CDS             3837..>4073
                     /locus_tag="VGC82_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422408.1"
                     /translation="MGPRRTAMLLMLAAGQACAQSAPSDSAANGKRIFVPSPLQWDSA
                     MLSYRPALRQTALDFANLTGGVAFGTSAGQVNLLL"
BASE COUNT          742 a         1294 c         1297 g          740 t
ORIGIN      
        1 gcccaagcat gtgaatcatg caggcaacaa tgggtcggac catttgcggt gtgcgtagtc
       61 aggcttgaaa tggtctagca aaatcgttcc ttggccagcg caaaaagtgc gcgtatagcc
      121 attgcctcgc cgccttccgg acgtcccggc cgggactggt cattccaggc atacagatcg
      181 aaatgcatcc aggggatgcc ggccttgacg aagcgctgca agtaaaggcc ggcgacgatc
      241 gctcctgcga gcggcttcga tgcgacgttg ttggtgtccg cgaccgggct ttttagccag
      301 tcctggtatc cgggccataa cggcaggcgc caaagcggat cgtggacctc gttgccgaac
      361 cgcattatgg cctcggccag gctgtcgtcg ttgctgaaca gtgccgggat gtccggaccc
      421 agggcgacgc gcgcggctcc ggtcaatgtc gcacaatcca ccagcaggga agggttttcg
      481 tccgacgctt catgcagcag atcgcacagt acaaggcggc cctcggcgtc ggtgttgccg
      541 acctctaccg tcagcccgga acgagtccgt atcacgtcgg atggacgcat ggcggttccc
      601 gagacggaat tttccacgca gccaatgcgc accgccagac gcaacggcag gtcagcttcc
      661 atgatggtac gtgcgagccc tagaacggtg gcagcgccgc ccatgtcctt cttcatccgc
      721 agcatgccgg cagacggctt caggtcatag cctccggtat cgaaacaaac ccccttgccg
      781 caaagcgaca gcaaaggaga cgcttcgtga gcggaactgc cgcgccagtg gaatgtcgcc
      841 acgcgcggcg gccgcaccga accacgcccc acggcagcga tcgttggata ggcttgctca
      901 agcgcggcat cggcggacag ctcggactgc gcgccgtagc gctttgccag cgccaccgtg
      961 aattccgcca gctccaccgg gcccagaatg tttgccggcg tgttgatcag atcgcggacc
     1021 atccaggttg ccgcggcctg agacaggctg gcttcgtgtc ccggagggac gaacagggac
     1081 gccggtttgc gtcccggcga aacgaagtgg ctgtatcgat acgccccaag gcagtaaccg
     1141 agaaccgccg cttcgctgtc aaagtcgcct tgttccaggc gccatgcgca aacatcgggc
     1201 agttgcatgg caagactgcc gaagacgaac ggcgaagtgt cggttccgat gccaaggacc
     1261 gcgccggcta cgccttccgg tccgggcagg aaacgcagtt ccccgccctt tgcctcgaat
     1321 ccggtgtcgc gcaggaaggc ggtctgaccg gatggcaacg tatcgagaaa gccggcaagc
     1381 cggtccggtc gcacgatgtg gaccgggcga gcacaatcgg gcgaagcggc gatgcagtcg
     1441 atgttctggt ccagcatggg cggaatcctc gtgtttcagt cctttgggtg gagaaggcga
     1501 agcagccgtg aatcccgcat catggaccgg ttcggagtgc gggaaacagc ggacctgttc
     1561 agcaatggtc catccaccgg ggttctggaa acgtgtcgct ccgcccgatc atgctttgca
     1621 tccgcctcca actgactgga tcgtgcggtt tcccccttgg catggccggc aaaggcgcca
     1681 gagcctgacg ccagagcctg acgatagtgt catgaccgtg cttgccaccc aagactttgt
     1741 ccgcacgcag gaaagtcctg agtgaccggc ccagcgccgg tcatgataca agcggcggag
     1801 accgcgcggt ggcgcttcac ctgacgaaca tgggcgaagc gtgccaccag ggtgttcggc
     1861 ggcaccctaa cccaggctcg atgccagcag ggacgtaacg cggcgggcga acgccgagga
     1921 atcggtcggc aagtcgcctt cctgcacctg tgcaaggtcc agaagggtct ccgatgcttc
     1981 ggcgatcagg ccgctgtcgt ccagtttcgc cgacagcgct tcgatcagct tgtgccgcgg
     2041 attgacctcc aggaccggtt ttgccggcat catcgcgcgc ccggcgcgcc gcatcagacg
     2101 ctgcatcgcg aggtcggggc ccttgccgtc cgatgccagc accacggcgc tatcggtcag
     2161 gcgggcggtg gagcggacgt ccgtgacgcg gccggcaagg ttggccttca tcgccgccaa
     2221 aagtggggag atgtccggct ggtcctcgcc cttgcccaga tcctcggcgg cctgcgtaac
     2281 gctgcgcagc ttcttgccct cgaaactgtc gagccgttct ggccagaacg agtcgatgga
     2341 gtccgacatc agcaacactt ctaggccctt ggaccggaac ccttccagtt gcggagagga
     2401 tttcagcgca tcggcatgat cgccagtcag gtagtagatc gtgtcctggc cttcctgcat
     2461 ccttgaaacg tagtcaggca gcgacgtcca accttcctgc gtggacgact tgaagcgcag
     2521 cagtgacgca atttcggcac gatgttccgc gtcctcccaa acgccctcct tcaggatcgg
     2581 accaaagttc ccccagaact tcgcgtagtc ctcggcatcc ttggtacggt ttttcagctc
     2641 cgacaacacg cgcccgatca tcgccttgcg gatgcgcgcc agcaccggcg tggtctgcag
     2701 catctcccgc gaaacgttca gcggcaggtc ttcggtatcg acgacaccgt tgacgaagcg
     2761 cagccagtgc ggcagcagtt ccgccttgtc ggtgatgaac atgcggcgga cgtgcagtcg
     2821 gacatgggcg tcccggtcgt tgtccaccgc gtcgaacggc ttcatgccgg gaatgaacaa
     2881 caggcagaaa tattccagcg tgccctcggc gcgccaatgc agcgtcgccc aggggtcgtc
     2941 gaagaacagg ccgaggtggc ggtagaattc cttgtaggac tcttccgtca cctccgactt
     3001 tgccttgcgc cagagtgccg tgccttcgtt ggccggctcg tccttgccat cccgagcgac
     3061 ggtgatcggc aaggtgatgt ggtcggccca tttgcggatc acggtctcca gccgtatcgg
     3121 ctccagatac tccgacgcgt cggacttcat atgcagcacg acatcggtgc cggcttgctc
     3181 gcgggtcgcc ggcgccaggg tgaacgcacc cgcaccttcc gacgcccagg taaatgcttc
     3241 ctcggcgccg gccttgcgcg atgtgacctc gacacggtcg gcgaccatga aggcggaata
     3301 gaagccgacg ccgaattggc cgatcaggct gggccggtcc tccggcttcg cggtcgccag
     3361 gttctgcgcg aaggcgcggg tgccggagcg ggcgatggta cccaggttgt cgatcagttc
     3421 ctgccgcgtc atgccgatgc cgtcgtccga gatcagcagc gtgctgccgt ccttgtcggg
     3481 cacgacgcgg atcttggcat cggtcggcgg cgccagggtt ggatcagtca aggattcgaa
     3541 ccggcggcga tccaccgcgt cggccgcgtt cgccaccagt tcccgcaaaa aaatctcccg
     3601 ctcggtatag agcgagtgca ccacgaggtc gagcaggcgg ccgacctcgg cgccgaatga
     3661 atgttgttcc aatacggtgt cgttcatacg tgacctcctt gcgcggcgcc atatgcggcc
     3721 aaatgccgga cgtttcaatg cgtggccgca tccggcccgg ccggatgaat acaaaacttc
     3781 caacgccaac gcggcatcgg taggttagcg ccgctggctg ctcagaggaa catggaatgg
     3841 gtccgcgccg aaccgcgatg ctgctgatgc tggcggcggg tcaggcgtgc gcgcagtcgg
     3901 caccgtccga cagtgcggcg aatggcaaac gcatcttcgt tccgtctccg ctgcagtggg
     3961 acagcgcgat gctgagctac cgccccgcgc tgcggcagac ggctttggac ttcgccaacc
     4021 tgaccggcgg cgttgcgttc ggcacgtcgg cggggcaggt gaatctgctt ctg
//
LOCUS       DASWQN010000226         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_155523, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000226 DASWQN010000000
VERSION     DASWQN010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155523"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VGC82_03700"
     CDS             complement(<1..416)
                     /locus_tag="VGC82_03700"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0422409.1"
                     /translation="MGTLDRCLREVGVACEPIVEDLLEEVRASGVIHADETPWWQGVL
                     GRWLWGVLSATTAVFHIGSRRHEEIRDLLGDAILGWLVTDGYGAYRDYARRQRCLAHL
                     IRKGIALAVHPDGIRFGEWLVRELRGLIKAVAEDKD"
     gene            complement(800..1000)
                     /locus_tag="VGC82_03705"
                     /pseudo
     CDS             complement(800..1000)
                     /locus_tag="VGC82_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6444 domain-containing protein"
BASE COUNT          274 a          529 c          511 g          244 t
ORIGIN      
        1 tggtccttgt cctcggcgac ggccttgatc aggccgcgca gttcgcggac cagccactct
       61 ccgaagcgga tgccgtcggg atggacagcc agggcgatcc ccttgcggat caggtgcgcc
      121 aggcaccgtt gacggcgggc gtagtcgcgg taggcgccgt acccatcggt gaccagccag
      181 ccgaggatgg cgtcgccgag caggtcacgg atttcctcgt ggcggcggct gccgatatgg
      241 aagacggcgg tggtggcgct cagcaccccc cacagccagc gcccgagcac gccttgccac
      301 cagggggtct catcggcgtg gatgacgccg gaggcccgca cctcttcgag cagatcctcg
      361 acgatcggtt cgcaggcgac cccgacctcg cgcaggcagc ggtcgagggt gcccaccgcc
      421 agcgccacgc caaaccaagt taataggaac tcacggatgc gtcggcggga ttgatggcgc
      481 caaggccgtg ataaaggcgg ccagcgacgg gccgaccagg ctgcgttcgg tcaacagcaa
      541 gtgccgcttc cctggaacga gagcacgccg atgccgggac tgtctcccgg ccgcacgcgc
      601 accgcatcac ctcgtagcgg tgctgcacgc agccgatccg gatgccgctg tcggtgcgct
      661 ccaactccag gaccgcgaac gcctccgccg tgcggacgac cgcgtcccac atctcgaacg
      721 ctatgccgca atgggcgcag accaccggcc agtggctctc ggtacgttcc gtcttcagcg
      781 gcgcgtcccg ccacgtgccc ttggctccgg gctgcttgcc gggcttgcgg ccagacggcg
      841 gcggctcctt cgcgcccggc ggcgtggcac cttcgctggg accaccgtcc ctgcggccga
      901 cgtcgctgcc cgtcgcatcg cccccaccgc caaaaggcgg gtcggacgag ggcggccgcg
      961 aactgttggt cgaattcaac gccaaacgct cgctcaactc catcgccatg gtgtgaagac
     1021 ggcaagtcaa ctcgacaagc tgcgccggtt ccaacgcctc gaatacggcg cggttcattc
     1081 gggtcaggtc aagttggtct ttggattcca tgtccccagc gaatcacaaa cgctgtcact
     1141 tggcaagccg ccacgccacg ccaatccttt gaaaaccata ggggagtcaa tcggttgcat
     1201 cctccccggc gggacgtgaa cggttacaac cgatcgggta cggatgcttc ggtttctcaa
     1261 ggaggggcgg gtcgcgggcg tcagtcaggc tgcggagatc ctggactata gtgtgcgcac
     1321 cgttcagcgc tggtggcagt gctatcgcga aggcggcctg gcggaacgca tcacgccgga
     1381 agcgtgggag ggcttgcagg cggagatgcg ggccggccac atcggcggga ggcgcagatt
     1441 tatctgcgcg acacctggca catcgggacg ccatcgatgc cctttccaag ctggcgcaag
     1501 accaagctga acgcccgcat caccgcaaca gccccgccga tcaggcggcg tttaaaaa
//
LOCUS       DASWQN010000227         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1343094, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000227 DASWQN010000000
VERSION     DASWQN010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343094"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VGC82_03710"
     CDS             <1..878
                     /locus_tag="VGC82_03710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0422410.1"
                     /translation="HLSRAIVKRFGPEAATFRFVLQTQGLFNAALAGCPFVIYTDHTI
                     ASHREYLASDPRLFRSKEFQELERKLFHRADRIVVSAAHVEQTLVRTYGCDPARVATI
                     LIGANVQEAVTSDCLDRYAAGRILFVGIDWERKGGPALLAAFDKVAARFPDATLTIAG
                     CSPAVSHPRIRTMGLIPRTEAAALLGNASIFCLPSVVEPSAVASVEAMAFKLPVVATT
                     VGGFPGMVVDGETGLLVPPNDPDALAGALATLLANPERARDMGQAGYRRSSELFTWDA
                     VGARLHAQIALLPLD"
     gene            1092..2084
                     /gene="gshB"
                     /locus_tag="VGC82_03715"
     CDS             1092..2084
                     /gene="gshB"
                     /locus_tag="VGC82_03715"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY0422411.1"
                     /translation="MNRPLRVAIQMDPIDSINIDADSTFALMLEAQSRGHTMWHYEVR
                     HMALKEGVSRPQGGRREERLFARGHKVKVARRHGAHHESGPSEMLDLGSMDVVLMRQD
                     PPFDMAYITATHMLEHIQPRTLIVNDPAAVRNAPEKIMVTHFPDLMPPTLVTWDLQAI
                     RSFRAEYKDIIVKPLFGNGGAGVFRIKPDDENLASLLEMHFARSREPLMVQRYEPAVR
                     LGDKRIILVDGEPMGALNRVPAAGEARSNMHVGGRPEKSPMTARDLEICAAIGPTLRD
                     QGMIFVGIDVIGDYLTEINVTSPTGLQEIARFDGVHIEKSIWDRIEQKVASRVA"
BASE COUNT          377 a          664 c          755 g          412 t
ORIGIN      
        1 gccatctgtc gcgggccatc gtcaagcgat tcgggccgga ggccgcgacc ttccggttcg
       61 tgttgcagac ccaggggttg ttcaatgccg ctttggcggg atgccccttc gtcatctaca
      121 ccgaccacac gatcgccagc catcgggagt acctggccag cgaccctcgg ctgtttcgct
      181 cgaaagagtt tcaggaactg gaacggaagt tgttccaccg ggccgaccgg atcgtcgtca
      241 gcgcggcgca tgtcgagcag acgctggtgc gaacctatgg ctgtgatccc gcacgggtag
      301 ccacgatcct gatcggcgcg aatgtccagg aagcggtcac gtcggactgc ctggatcgtt
      361 atgcggccgg ccgtatcctg ttcgtgggga tcgattggga gcgtaagggc gggccggccc
      421 tgctggccgc cttcgacaaa gtggccgccc ggttccccga cgcaacgctc accatcgctg
      481 gttgctcgcc agcggtatcg catccgcgca tccgcacgat gggccttatt ccccgcaccg
      541 aggccgcggc gttgctgggg aatgcgtcga ttttctgcct gccgagcgtg gtcgagccgt
      601 cggcggtggc ctcggtggag gcgatggctt tcaagctgcc ggtcgtcgcg acgacggtcg
      661 gcggctttcc gggcatggtc gtcgatggtg aaacgggact tctggttccg ccgaacgatc
      721 ctgatgcgct ggctggcgcg ctggcgacgc tgcttgctaa tcccgaacgg gcccgcgaca
      781 tggggcaggc cggctaccgg cgcagcagcg aactgtttac ctgggatgcc gttggcgctc
      841 ggttgcatgc acaaatcgcg ttgctgccgc tggattgagc tctgtgtttg gctggctggc
      901 gccgcgcctt cccctgtccc ggtccatccc ttagtgtcgc gaaccggacg ttcgctgtca
      961 ccagcaaacg ccggtttcga aatacttgct cttggtttgc agtggcctgc cgatccgctg
     1021 cgtccgccgt gtgatgcggc gagcttcggg gctggatggc aggccggctt tcaacgactg
     1081 gggataatcg catgaaccgc ccgctccggg tcgccatcca gatggacccg atcgacagta
     1141 tcaacatcga cgcggattcg acgttcgcgc tgatgctgga ggcgcagtcg cggggccaca
     1201 cgatgtggca ctacgaagtg cggcatatgg cgctgaagga aggcgtgtcc cggccgcagg
     1261 gaggcaggcg ggaggagcgg ctgtttgccc gcgggcacaa ggtgaaggtg gcgcggcggc
     1321 atggcgcgca tcatgaatcg ggcccgagcg aaatgctcga cctgggcagc atggacgtgg
     1381 tgctgatgcg gcaggatccg ccgttcgaca tggcctatat cacggcgacg catatgctgg
     1441 agcacatcca gccacggacg ctgatcgtca atgatccggc ggcggtgcgc aatgcgccgg
     1501 aaaagatcat ggtcacgcat tttcccgacc tgatgccgcc gacactggtg acctgggatc
     1561 tgcaggcgat ccgctcgttc cgggcggaat acaaggacat catcgtcaag ccgctgttcg
     1621 gcaatggcgg tgctggggtg ttccgcatca agccggatga cgaaaacctg gcgtccctgc
     1681 tggaaatgca ttttgcccgg tcgcgcgaac cgctgatggt gcagcgctat gaaccggcgg
     1741 tgcggctggg cgacaagcgg attatcctgg tcgatggcga acccatgggc gcgttgaacc
     1801 gcgttccggc ggccggggaa gcgcggtcca acatgcatgt cgggggccgg ccggaaaaat
     1861 ctccgatgac cgcgcgcgac ctggagatct gcgcggcaat cgggccgacc ttgcgggatc
     1921 agggcatgat cttcgtcggc atcgacgtga tcggcgacta cctgaccgag atcaatgtca
     1981 cctcgccgac cgggttgcag gagattgccc ggttcgacgg ggtccacatc gaaaagtcga
     2041 tctgggaccg gatcgagcag aaggtagcgt caagggtcgc gtaaccggcg agacaaagcg
     2101 gcatccgccg cggaagccgg ggccctctgt ttagaccgac ccgtgtttgt ttggaccgac
     2161 ctgcgcttgt gcccggagcg gacggtctgc ccgaaacaaa aaaaacgg
//
LOCUS       DASWQN010000228         5235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2267054, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000228 DASWQN010000000
VERSION     DASWQN010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267054"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..821)
                     /gene="urtD"
                     /locus_tag="VGC82_03720"
     CDS             complement(75..821)
                     /gene="urtD"
                     /locus_tag="VGC82_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEY0422412.1"
                     /translation="MSEGDTLLYLDGVTVTFDGFRALNALSLTLDEGEMRAVIGPNGA
                     GKTTMMDVITGKTRPDSGKVVFSKDADLTKMDEPAIASMGIGRKFQKPTVFEPHTVWD
                     NLLLALAGDRGARFTLFARETAEEKRRIESILETTRLTDQRHRRSADLSHGQKQWLEI
                     GMLLAQEPQLLLVDEPVAGMTDAETEQTATLLREINRTRSVVVVEHDMAFVRALGVKV
                     TVLHEGSVLSEGSIDFVSDDPKVIEVYLGR"
     gene            complement(818..1936)
                     /gene="urtC"
                     /locus_tag="VGC82_03725"
     CDS             complement(818..1936)
                     /gene="urtC"
                     /locus_tag="VGC82_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEY0422413.1"
                     /translation="MTLDRTGYLTIGGVLGVALILAMLNQMTAAGSSMHVPTYIVALA
                     GKYLCFAMLALAVDLVWGFAGILSLGHAAFFALGGYAMGMYLMRQVGTRGVYGNAMLP
                     DFMVFLGWKELPWYWFGFEWFGFAMLMAVLVPAVLAGVFGWLAFRSRVTGVYLSIITQ
                     ALTYALLLAFFRNDMGFGGNNGLTDFKDILGFNVQADSTRSALLVISALLLCGQFLVA
                     RFVVDSRFGKILIAVRDTESRTRFLGYRPESYKLVVWVLSAVMAGIGGALYVPQVGII
                     NPSEFAPGNSIEAVIWVAVGGRGTLVGAILGAVLVNFGKTWFTGALPEIWLYALGAMF
                     IFVTLFLPRGVMGLFRARTAKEPEPEPPAVAAAHEVTG"
     gene            complement(1933..3522)
                     /gene="urtB"
                     /locus_tag="VGC82_03730"
     CDS             complement(1933..3522)
                     /gene="urtB"
                     /locus_tag="VGC82_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEY0422414.1"
                     /translation="MARLLVLLLFCGLWQPVVAYADDPLNDPLAELASGAFNGVRQGV
                     EDLALSGNPRAATILSALQGGQLYFTPGHTLLIKGADDHYVDAATGAPIADAGFGVKL
                     VRLNNSVRGAIDAALGSLRLFDPSASVRLEAAEAVFQSHDPAAIPALDRALAKETDAS
                     VKRRMEQARAAAVLYDRDAQQDARMAAVGVLRARGDQDSRSLLSTLAGQSGPVADAAG
                     AAVISIDRVLQIWSIAQSVYYGISLGSVLLLAAAGLAITFGVMGVINMAHGEMVMIGA
                     YVTFVVQQFMHAYLPSLYGISLLLAIPLAFLTSGLVGVVVERSLIRWLYGRPLETLLA
                     TWGLSLVLQQVIRSLFGANNRDVDTPGWMSGATQLGGLTLTWNRMYIILFAFLVVAAL
                     MAALRYTPLGLRMRAVTQNRRMAAAMGIRTPWIDALTFGLGSGVAGIAGVALSQIDNV
                     SPNLGQGYIIDSFMVVVFGGVGNLWGTTLGALTLGIVNKFLEPIAGAVLGKIVVLVLL
                     ILFVQRNPRGMFPLKGRAVEA"
     gene            complement(3593..4930)
                     /gene="urtA"
                     /locus_tag="VGC82_03735"
     CDS             complement(3593..4930)
                     /gene="urtA"
                     /locus_tag="VGC82_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEY0422415.1"
                     /translation="MSLDEPILSRRGLGRLGVAAATLAAVGAIKAATAPAEAQGTGGT
                     IKVGILHSLSGTMAISETTLKDVMLMLIEQQNAKGGLLGRNLEAVIVDPASNWPLFAE
                     KARQLISVDKCAATFGCWTSVSRKSVLPVFEELNSILFYPVQYEGQECSRNVFYTGAA
                     PNQQAIPAVDYLMAENKVKRWVLAGTDYVYPRTTNQILEAYLKSKGVAADDIMINYTP
                     FGHADWQNIVASIKKFGSGGKKTAVVSTINGDANVPFYKELGNQGIKATDIPVVAFSV
                     GEEELAGIDTKPLVGHLAAWNYFQSMDTPENKDFIASWKKFTKNPKRTTNDPMEAHYI
                     GFNMWVKGVEKAGTTNPDEVIEALLGVSVPNLSGGYSAMMPNHHITKPVLIGEVQADG
                     QFNTVSQTPGLVVAQEWSPYVADTKDLIGDWRAPLKCGAFNVKTGTCTAGKKA"
BASE COUNT         1046 a         1727 c         1551 g          911 t
ORIGIN      
        1 gaaacctgcc gcagcgcgat cgccgcgccg tagtgcagac tgactttctc gacttccagc
       61 atcagccccc cgccttatcg cccaagatac acttcaatga ccttgggatc atcgctgaca
      121 aagtcgatcg agccctccga cagcaccgaa ccctcgtgca gcacggtcac cttcacaccc
      181 agtgcccgga cgaaagccat gtcatgctcg accacgacca cgctgcgcgt ccggttgatc
      241 tcgcgcagca aggtggcggt ctgctcggtt tcggcgtcgg tcatgcccgc caccggttca
      301 tccaccagca gaagctgagg ctcctgcgcc agcagcatgc cgatctcgag ccattgtttc
      361 tgtccatgcg acaggtcggc ggaacgccgg tgccgctgat cggtcaggcg cgtcgtttcc
      421 aggatggact cgatccgccg cttctcttcc gccgtctccc gcgcgaacag agtaaatcgc
      481 gcgccacgat cgccggccag cgcgagcagg agattgtccc agaccgtatg aggctcgaac
      541 accgtcggct tctggaattt tcggccgatc cccatggagg cgattgccgg ctcgtccatt
      601 ttcgtgaggt cggcgtcctt cgagaacacc acctttccgg aatcgggccg cgtcttgccg
      661 gtaatgacat ccatcatcgt tgtcttgccg gcgccgttgg ggccgatcac ggcgcgcatt
      721 tcgccctcgt ccaatgtcag ggaaagtgcg ttcagcgcac ggaacccgtc gaacgtgacc
      781 gtcacgccgt cgaggtaaag cagcgtgtcg ccctcgctca tcccgtcacc tcatgcgccg
      841 ccgccaccgc cggaggctcc ggttccggct ccttcgccgt cctggcccgg aacaaaccca
      901 tgacgccccg cggcaggaac agcgtcacga aaatgaacat cgcgcccagg gcataaagcc
      961 agatttcggg cagtgcgccg gtaaaccagg tcttgccgaa gttgaccagg acggccccca
     1021 ggatggcacc gaccaaagtg ccgcggccac ccaccgccac ccagatcacc gcctcgatcg
     1081 aattgcccgg cgcgaattcg ctcgggttga tgatgcccac ctggggcacg tagagcgcgc
     1141 cgccgatgcc cgccatcacc gccgacaaga cccacaccac aagcttgtac gactccggcc
     1201 gatagccgag gaatcgtgtc cggctttcgg tatcgcgcac ggcgatcaga atcttgccga
     1261 accgggagtc cacgacgaac cgcgcaacca aaaactggcc gcaaagcaac agcgcggaga
     1321 tcaccagcaa ggcggagcgc gtcgaatcgg cctgaacatt gaacccaagg atatccttga
     1381 agtcggtcag gccgttattg ccgccaaacc ccatgtcgtt gcgaaagaac gccagcagca
     1441 gcgcgtaggt cagagcctgc gtaatgatcg acaggtacac gccggtgacc cgtgagcgaa
     1501 acgccaacca accgaaaacg ccggccagta ccgccggcac cagcaccgcc atcagcatgg
     1561 cgaagccgaa ccattcgaag ccgaaccagt accaaggcag ctccttccag cccaggaaca
     1621 ccatgaaatc cggcagcatg gcgttgccat aaacgccccg ggtgccgacc tgtcgcatca
     1681 ggtacatgcc catcgcatag ccacccaagg cgaagaacgc ggcgtgcccg agcgaaagga
     1741 tgccggcgaa accccagacg aggtccacgg ccagggccag catcgcgaag cacaggtact
     1801 tcccggcaag tgccacgata taggtcggca catgcatgga cgatcccgcg gcggtcatct
     1861 ggttcagcat tgccaggatc agcgccaccc cgagaacacc gccgatcgtc aggtatccgg
     1921 tccggtcgag cgtcatgcct ccaccgcccg tcccttcaat gggaacatgc cgcgtggatt
     1981 acgctgcacg aacaggatca acagcaccag aacgacgatc ttgcccagca cggcgccggc
     2041 gatcggttcc aggaatttat tgacgatgcc caacgtgagg gcacccaggg tcgtgcccca
     2101 caggttcccg acaccgccaa acaccaccac catgaaactg tcgatgatgt agccctggcc
     2161 caggttcggc gacacgttgt cgatctgtga cagcgcgaca ccggcgatac cggcaactcc
     2221 cgatccaagc ccgaaggtca gcgcatcgat ccaaggcgtc ctgatcccca tcgctgccgc
     2281 catccggcga ttctgggtca ccgcgcgcat gcgcaaaccc agcggcgtgt agcgcagcgc
     2341 ggccatcaat gccgcgacca ccaggaacgc gaacagaatg atatacatcc ggttccaggt
     2401 cagcgtcagg ccgcccaact gggtcgcgcc gctcatccag cccggtgtat cgacatcgcg
     2461 gttgttggcg ccgaacagcg accgaatcac ctgctggagg accagagaca ggccccaggt
     2521 cgcgagcaac gtttccagcg gccggccata caaccagcgg atcaggctgc gctccacgac
     2581 gacgcccacc aggccggagg tcagaaacgc cagcggaatc gccagcagca ggctgatgcc
     2641 gtacagcgac ggcaggtaag cgtgcatgaa ctgctgcacg acgaatgtga cataggcccc
     2701 gatcatcacc atctcgccgt gcgccatgtt gatgacgccc atgacaccga atgtgatcgc
     2761 caaaccggct gccgccagca acaagaccga gccgaggctg atcccgtaat agacgctttg
     2821 cgcgatgctc cagatctgca gcacgcggtc gatggagatc acggccgcac cggcggcatc
     2881 cgcgaccgga ccgctctggc ctgcgagcgt cgaaagcagg ctgcgcgaat cctgatcgcc
     2941 tcgtgcgcgc agcacaccga cagctgccat gcgtgcatcc tgttgcgcat cgcggtcgta
     3001 caacaccgcc gccgcccgtg cctgctccat tcggcgcttg accgaagcat cggtttcctt
     3061 ggccaaagcc cggtcgagcg ccgggatcgc ggctgggtca tgcgactgga acaccgcctc
     3121 cgctgcttcc agccgcaccg aagcgctggg gtcgaacaac cgcaggctgc ctagtgccgc
     3181 gtcgatcgcg cctcggaccg agttgttcag acgcacgagt ttgacgccaa aaccggcatc
     3241 ggcgatgggg gcgccggtgg ccgcgtcaac gtaatggtcg tcggcgccct tgatcagcag
     3301 cgtgtgcccg ggggtgaagt acagctgacc gccctgcagc gccgagagga tcgttgccgc
     3361 gcgagggttg cccgacagcg cgaggtcttc gacgccttgc cgcacgccgt tgaaggctcc
     3421 ggacgcaagc tctgccagtg ggtcgttcag cgggtcgtcg gcatacgcca ccacaggctg
     3481 ccaaagccca caaaacaaca gtagaaccag caagcgcgcc atgtattgat ccaataaaag
     3541 aagaaggggg cggccgggag gaacagccgc cccctatctg gcaatcaggc cgtcaggcct
     3601 tcttgccggc ggtgcacgtg ccggtcttca cgttgaacgc accgcatttc aatggcgcac
     3661 gccaatcgcc gatcaggtcc ttggtgtcgg cgacataggg cgaccattcc tgagcaacaa
     3721 caagaccggg agtctgcgac accgtattga actgcccgtc ggcctgaacc tccccgatca
     3781 ggaccggctt agtgatgtgg tggttcggca tcatcgcgga gtatccgccc gaaaggttcg
     3841 gcaccgaaac gccaagcagt gcttcgatga cttcatcggg gttggtcgtt ccggcctttt
     3901 cgacgccctt cacccacatg ttgaagccga tgtagtgcgc ctccatcgga tcgttggtgg
     3961 tgcgcttggg gtttttggtg aacttcttcc aggaagcgat gaagtccttg ttctctggcg
     4021 tgtccatgct ttggaagtag ttccacgccg cgagatggcc gaccaacggc ttggtgtcga
     4081 tgccggccag ttcttcctcg ccgacgctga acgcgacgac gggaatgtcg gtcgccttga
     4141 tgccctggtt gccaagttcc ttatagaacg gcacgttggc gtcaccgttg atggtggata
     4201 ccacggcggt tttcttgccg ccggagccga atttcttgat cgacgccacg atgttctgcc
     4261 agtcggcatg tccgaacggg gtgtagttga tcatgatgtc gtcggcggcg acgcccttgg
     4321 acttcagata ggcttccagg atctggttcg tcgtgcgcgg atagacatag tcggtgccgg
     4381 ccaggaccca gcgtttcacc ttgttttccg ccatcaggta atctaccgcg gggatcgcct
     4441 gctggtttgg cgccgcaccg gtgtagaaca cgttgcggct gcattcctgg ccctcatact
     4501 gcacgggata gaacaggatg ctgttcaatt cctcgaacac cggcagcacg gacttgcgcg
     4561 acaccgaggt ccagcaaccg aacgtggcgg cgcatttgtc gaccgaaatc aactggcgag
     4621 ccttttcggc gaacagcggc cagttggaag ccggatcgac gatcaccgct tccaaattcc
     4681 tgcccagcag gccgcccttg gcgttctgct gctcgatcag catcagcatg acgtccttca
     4741 gcgtggtctc gctgatcgcc atggtgccgg acagggaatg gaggatgccg accttgatgg
     4801 tgccgccggt tccctgcgct tccgccgggg ccgtagcagc tttgatggca ccaaccgcgg
     4861 caagcgtcgc ggcggccaca ccgagacggc cgaggccgcg acgggaaaga atgggttcgt
     4921 ccagtgacat cagtgcctgt tcctgtgcct gttgttgttt cagtttcccc gtctcacgga
     4981 ggcggaggca cggaatgcaa cgaatgtgcc agagggctgg ctgtccggcc atcggccatc
     5041 cctatccgac ctgggtgccg ggaggacaca ttccatggat caggttgacg gcggaggcac
     5101 aacgggatca cggagcgcga acgacctgtc ctgcatcctg ctccacaaaa gcggtttctt
     5161 ggattggcgt tcggggacag ctaagtgcat cgggccgggc gatcctgccc agcaatgtcg
     5221 ttgcagttgc ggcct
//
LOCUS       DASWQN010000229         5413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1211547, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000229 DASWQN010000000
VERSION     DASWQN010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211547"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VGC82_03740"
     CDS             complement(<1..206)
                     /locus_tag="VGC82_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422416.1"
                     /translation="MRSRPPWSGVPDDRTGELRGLAAAIAVVTLIRLVMAAIVPLAPD
                     EAYYWIWSRALAPGYLDHPPMVAL"
     gene            complement(169..1308)
                     /locus_tag="VGC82_03745"
     CDS             complement(169..1308)
                     /locus_tag="VGC82_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0422417.1"
                     /translation="MPDVTIESESTRLPRDKAGPVLSVVVPCYNERPNVAPMIAKLDA
                     ALAGIAWEVIYVDDNSPDGTAQEVRRIAQHDPRVRCIRRIGRRGLASAVIEGALSSSA
                     TYVAVIDGDLQHDETRLPVMLAALQAGDYDIAVASRHVEGGDSAGLSSRFRQVLSDGG
                     IRLAQAFLPVRLSDPMSGFFMLPRALFEDLARGLNGQGFKILVDLVLSSAAPLRVLEV
                     PARFRERAAGESKMDALVMIQFGGLLLDKVFGGLLPLRFFSFALVGALGIVVHLIALG
                     LLRQTTVLGFEAEQTIATVIAMVFNFQLNNLITYRDQQLKGPRLWRGLLLFMVVCGFG
                     AVANVGIAQMLYEQHTTWTVAGGVGAMIGVVWNYAISATLVWRSR"
     gene            complement(1417..2109)
                     /gene="pdeM"
                     /locus_tag="VGC82_03750"
     CDS             complement(1417..2109)
                     /gene="pdeM"
                     /locus_tag="VGC82_03750"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEY0422418.1"
                     /translation="MIAAPVHLAGERVMLDPMGALFWPEQRLLAVSDLHLEKGSSFAR
                     RGMLLPPWDTHATLDRLTLLLRRWSPDVVVALGDSFHDAGGSGRLLATEQRRLNAMSK
                     THRFIWVQGNHDPIPPDGLGGDWVETYSAGAMLFRHQAVMAANPGEVVGHHHPKATIP
                     ARAGSVSRPCFVFDGSRLMMPAFGAYTGGLDVRDPAINRLFPRGGRVYLLGKERLFSF
                     EIGRGAALRTAT"
     gene            complement(2106..4628)
                     /locus_tag="VGC82_03755"
     CDS             complement(2106..4628)
                     /locus_tag="VGC82_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEY0422419.1"
                     /translation="MAGTLPEPFATWFAARGWTPRPHQLAMLEAARAGESVLLVAPTG
                     GGKTLAGFLPSLIDLAASPRPGLHTLYLSPLKALATDITRNLTRPVQDMRLPITIEAR
                     TGDTPANRRARQKAAPPHMLLTTPESLAVLISLPDAPAFFGSLACIVMDEVHALAGTK
                     RGDQLALGVARLATISPKARRVGLSATVAHPDAIQAYTGACRRIEVADGAPPEISITL
                     PKGRLSWSGHMGLEAAPEVMAHIRAASMTIVFVNTRAQAELMFQALWKLNETTLPIAL
                     HHGSLEVEQRRKVEAAMAAGSLRAVVATSSLDLGIDWGGVDQVLQIGAPKGVSRLLQR
                     VGRANHRMDEASKAVLVPANRFEVLECEAAILGVAARELDGDPPRPGGLDVLAQHLLG
                     LACSTPFLPDDAYQETVSAAPYAGLSREDFDDVLRFVEDGGYALRAYEQWKKLFRDSE
                     GRMHVRNARTAVVHRMNIGTIVEAEVLKVRLVGKRRFGPTLGEIEEYFANMLRPGDTF
                     MFAGRLLRFMRVFENAVECMEGGDGDPMVPAYAGGRLPLTTNLADRVRGLLQSPEKWD
                     LFPDQVREWLRLQRAVSRMPGRDDLLVETFPRGDRWYLVAYCFEGRNAHQTLGMLVTK
                     RMERAGFAPLGFVATDYVLGIWSANPPRDVATLFDEDMLGDDLESWMADSSMLKRTFR
                     NVAVIAGLIQRNQPGAEKTRRQVTVNSDLIYDVLRRHQPDHILLRATRADATTGLIDA
                     GRIAGMLARVKGKIVHMVLSRVSPLAVPVLLDIGRESVRTDTDEETLLAEAEAIIAEA
                     TGGAEPRYVPAVHRANVARRTTGARKTILPRHPVGRQSDLFS"
     gene            4929..5054
                     /locus_tag="VGC82_03760"
     CDS             4929..5054
                     /locus_tag="VGC82_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422420.1"
                     /translation="MLLSPLTIVLPVAPGTVGFSFAGVDSFTENLLPVVSAGLAL"
BASE COUNT          962 a         1773 c         1772 g          906 t
ORIGIN      
        1 cagagtgcga ccatcggcgg atgatccagg tatcccggcg ccagcgcacg cgaccagatc
       61 cagtaatagg cctcgtccgg agccagcggg acgatcgccg ccatcaccag gcggatgagg
      121 gtgaccaccg cgattgcggc ggccaatccc ctcagctcac cggtcctgtc atcgggaacg
      181 ccagaccagg gtggccgaga tcgcatagtt ccagaccact ccgatcatcg ccccgacgcc
      241 ccctgcaacg gtccaggtgg tgtgctgctc gtacagcatc tgcgcgatgc cgacattggc
      301 gaccgcgccg aagccgcaga cgaccatgaa cagcagtaac ccccgccaaa gccgcggacc
      361 tttcagttgt tggtcgcggt aggtaatcag gttgttgagt tggaaattga acaccatggc
      421 gatcaccgtg gcgatcgttt gttcggcttc gaagcccagc acggtggtct gccgaagcag
      481 gccgagggcg atcagatgaa ccacgatacc gagcgcgcca accagggcga aggagaagaa
      541 gcgcagcggc agcaatccac cgaacacctt gtccagcagc agcccgccga actggatcat
      601 caccagggcg tccatcttgc tctcgcccgc ggcccgttcg cgaaaccggg cgggaacttc
      661 caatacgcgc aacggtgcgg cgctgctgag aacgagatcc accaggatct tgaagccctg
      721 gccattgagg ccgcgggcga gatcctcgaa caacgcgcgc ggcagcatga agaagccgct
      781 catggggtcg ctcaggcgca ccggcaggaa ggcctgggca agccggatcc cgccgtctga
      841 gagcacctgc cgaaagcggc tcgacagccc ggcgctgtcg ccgccctcga cgtggcggct
      901 ggcgacggcg atgtcgtagt cgccggcctg cagtgccgcg agcatcaccg gcaaccgggt
      961 ttcgtcgtgc tgcaaatcgc catcgatcac cgcgacatag gtggccgaag aggacagcgc
     1021 gccctcgatt accgccgaag ccaggccgcg ccggccgatc cgccggatgc accgcacccg
     1081 cgggtcgtgc tgagcgatcc gacgtacctc ctgggcggta ccgtcaggac tattatcgtc
     1141 aacgtagatc acttcccagg cgataccggc gagcgcggcg tccagtttgg cgatcatcgg
     1201 cgccacattc gggcgctcgt tgtagcatgg cacgacgacg ctcagcaccg gaccggcctt
     1261 gtcccgcggc aggcgggtcg attccgattc gatcgtcacg tcgggcatcc gaattcgact
     1321 ccacgcgcaa gtgcggtcgc ggagatacaa gacggacggc caaggtcaag gggaagtgga
     1381 cttcgcatag gctggcgttc gccagggcac accaggtcac gtcgcggttc gcaatgcggc
     1441 accgcgtccg atctcgaagc tgaacagccg ttccttgccg agcaggtaca cccgcccgcc
     1501 gcgcgggaac agacggttga tcgccgggtc gcgcacatcc agcccaccgg tataggcacc
     1561 aaatgccggc atcatcagcc ggctgccatc gaatacgaag catgggcggc tgaccgaacc
     1621 cgcgcgcgcc ggaatggtcg ctttcgggtg gtggtggcca acgacctccc cggggttggc
     1681 cgccatgacg gcctgatgcc ggaacagcat cgcgccggcg ctataggttt cgacccagtc
     1741 gccacccaaa ccgtccggcg ggatcggatc atgattgcct tgcacccaga tgaaccggtg
     1801 cgtcttgctc atcgcattca gccgccgctg ttcggttgcc agcaggcgcc ccgacccccc
     1861 ggcatcgtgg aacgaatccc ccaatgccac cacaacgtcg ggcgaccacc ggcgcagcag
     1921 caacgtcagc cggtcgagcg tcgcgtgagt atcccacggc ggcaacagca tcccgcggcg
     1981 ggcgaacgac gaacccttct ccagatgcag atcggacacc gccagcagcc gctgctccgg
     2041 ccagaacaac gcgcccatcg ggtcgagcat cacccgttct ccggccaggt ggaccggcgc
     2101 ggcaatcacg agaacaggtc cgactgccga cccacgggat ggcgcggcag aatggttttc
     2161 cgcgccccgg tcgtccgccg ggccacattg gctcggtgta ccgccggaac atagcgcggt
     2221 tcggcgcctc cggtcgcttc ggcgatgatg gcttcagcct cggccagcag ggtttcttcg
     2281 tcggtgtcgg ttcgcacgct ttcccgtcca atatccagca gcacgggcac cgccaacggc
     2341 gaaacccgcg acagcaccat atgcacgatc ttgcctttca cccgcgccag catgcccgcg
     2401 atgcggccgg cgtcgatcaa cccggtcgtg gcatcggctc gagttgcccg cagcaggatg
     2461 tggtcgggct ggtggcggcg cagcacgtca tagatcaggt cgctgttcac cgtaacctgc
     2521 cggcgggttt tttccgcccc cggctgattg cgctggatca gcccggcgat caccgcgaca
     2581 tttcgaaagg tccgcttcag catggagctg tcggccatcc atgattccag gtcatcgccg
     2641 agcatatcct cgtcgaacaa cgtggccacg tcccgcggcg ggttggccga ccagatcccc
     2701 aggacatagt cggtggcgac gaagcccagc ggggcgaagc cggcccgctc catccgcttg
     2761 gttaccagca tgccgagcgt ttggtgggcg ttgcggcctt cgaagcagta ggcgacgagg
     2821 taccagcgat cgccacgagg gaacgtttcg accagcagat cgtcgcgtcc gggcatgcgc
     2881 gagacggcgc gttgcagccg cagccactcg cgcacctgat cggggaacag atcccatttc
     2941 tccggcgatt gcagcagccc gcggacgcgg tccgcgaggt tggtggtcag cggcagtctt
     3001 ccgccggcat aggccggcac catcgggtcg ccatcgccgc cttccatgca ttcgacggca
     3061 ttttcgaaca cgcgcatgaa tcgaagcagc cttccggcaa acatgaaggt gtcgcctggg
     3121 cggagcatgt tggcgaaata ttcttcgatc tccccgagcg tgggaccgaa gcggcgcttg
     3181 ccgacaagcc ttaccttcag cacctccgcc tcgacgatgg tgccgatatt catccggtgc
     3241 acgacggcgg tgcgggcgtt acggacgtgc atccttccct cgctgtcgcg gaacaatttc
     3301 ttccattgct cgtaggcgcg cagagcgtag ccgccatcct cgacgaagcg caggacgtcg
     3361 tcgaaatctt cgcgcgacag gcccgcgtat ggagcggcag agaccgtctc ttgataggcg
     3421 tcatcgggca ggaacggtgt actgcaagcc agccccagaa ggtgttgagc cagcacgtcc
     3481 aggccgccgg gccggggcgg gtcgccgtcc agttcgcgtg cggcgacgcc aaggatggca
     3541 gcctcgcatt ccagaacctc gaatcggttg gcgggcacca ggacggcctt cgatgcctcg
     3601 tccatgcggt ggttggcgcg tccgacacgc tgcaacaggc gggaaacgcc tttcggcgca
     3661 ccgatctgaa ggacctgatc gaccccgccc caatcgatgc cgaggtccag cgacgacgtt
     3721 gccacgaccg cgcgcagcga cccggcggcc atcgcggctt cgaccttgcg gcgctgctcg
     3781 acctccaggg agccgtgatg cagcgcgatc ggcaacgtgg tttcgttgag tttccacagc
     3841 gcctggaaca tcaactccgc ctgcgcgcgg gtgttgacga agacgatggt catgctggcg
     3901 gcccggatgt gcgccatgac ctcaggggcc gcctccaggc ccatgtggcc ggaccaggac
     3961 aggcggccct tcgggagggt tatcgatatt tccggcggag cgccgtcggc gacttcgatc
     4021 cggcggcagg cgccagtgta ggcctggatc gcgtcgggat gtgcgacggt cgccgacagg
     4081 ccgacccggc gggccttcgg ggatatggtg gcgaggcggg cgacgccgag agcgagttgg
     4141 tcgccgcgct tggtgcccgc cagcgcgtgc acctcatcca tgacgatgca ggcgagggag
     4201 ccgaagaaag ccggcgcgtc gggcagcgaa atcagtacgg cgaggctttc cggggtggtc
     4261 agaagcatat gcggcggtgc ggctttctgc cgagcgcggc ggttggcggg ggtgtcgccg
     4321 gtgcgggcct cgatcgtgat cggaagccgc atgtcctgca ccgggcgggt caggttgcgg
     4381 gtgatgtcgg tggcgagggc cttcaacgga ctcagataca gggtgtgcag gccggggcgc
     4441 ggcgacgcgg cgagatcgat caggctgggg agaaagccgg cgagggtctt gccgccaccg
     4501 gtcggggcaa ctagcagcac gctttccccg gcacgggccg cttccagcat ggccaactgg
     4561 tgcggacgcg gcgtccaccc gcgggcggcg aaccatgtcg cgaatggttc cggtaatgtt
     4621 cctgccattg caggagataa gcatgcgcag gcggcggaga caaatggttt caggcggtct
     4681 gcgacgccct tcctcggggt gcctggcctg gagcacgtcc ctcggtccaa ggccagtggc
     4741 gcagatccca ctgcctcatc gtgcggttag cggctccgac tgacacgtag agcccggcgc
     4801 gcactgcata tgcaggcgca aacgcgcata catatgcagc agcaacgtgc ttgcaggttc
     4861 agcccggggc gtcgctcgcc gatccacccg tcttctgttc ggtcgttccg cggcggtcga
     4921 ggctgatagt gcttctgtcg cccttgacga tcgttctgcc cgtcgcgccc ggcaccgtcg
     4981 ggttctcatt cgccggagtg gattcattta ccgaaaacct gctgccggtt gtcagcgcgg
     5041 ggttggcgct ctgatcggat ccgtatgtcg gcgtcgcgat cggggcgtgt cctccgcctg
     5101 aattgccggg attggtatct tgcgcccaaa ccgcgacgac gggcgtcaag gacagcgcgg
     5161 ccgctagggc ccaacaaaaa gtgctcttca tcaaagtatc tccttttgag caatgaagat
     5221 gggtttgagg cccaactccg tcaatacgtg ccgcgccgca atcgtcggac gaagggtgtc
     5281 tggttgcgtc ggcgttcgac tgcacagttc gagtaattat cgtgatggag cctgccttaa
     5341 agcctgccgg gcatccgatc cgtcacaaat tccgcttctg cttgacgccc ctggcgctca
     5401 tgaaagtgat gac
//
LOCUS       DASWQN010000230         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1079359, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000230 DASWQN010000000
VERSION     DASWQN010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079359"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VGC82_03765"
     CDS             complement(<1..607)
                     /locus_tag="VGC82_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY0422421.1"
                     /translation="MDGSIALDDTWTVAGRTFRSRLIVGTGRYKDLEETGRAIEASGA
                     EIVTVAVRRVNLSDSGAPMLQDYVDPKKYTYLPNTAGCFTAKDAVRTLRLAREAGGWN
                     LVKLEVLGPPPLLYPDMPGTFEAAEALIKDGFEVMVYCADDPLAAKRLQEMGCVAIMP
                     LGAPIGSGLGIQNPAMISMMMEHVTVPFLVDAGVGTASDAAI"
     gene            complement(610..918)
                     /gene="thiS"
                     /locus_tag="VGC82_03770"
     CDS             complement(610..918)
                     /gene="thiS"
                     /locus_tag="VGC82_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY0422422.1"
                     /translation="MEAIIGGQTSASLPQTLPPRRRRTILTGMASSITLTLNGESRIF
                     EGPTDGPLSVSALLIVLGLDTKKVAVERNEEIVPRSRYAETWLASGDSLEIVHFIGGG
                     "
     gene            1034..1471
                     /locus_tag="VGC82_03775"
     CDS             1034..1471
                     /locus_tag="VGC82_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEY0422423.1"
                     /translation="MTRIPIDPDTIRALAAVLVETGLTEIEIADKDSRIRVVRGGGVA
                     TAVMPVMTPAPAVAAPAVPDMASHPGAVLSPMVGVAYLAPEPGAPAFVNVGQQVSAGQ
                     TLLLIEAMKTFNQIKAPKSGTVAAILVQSGAPVEYGEVLIVLE"
     gene            1476..>2469
                     /locus_tag="VGC82_03780"
     CDS             1476..>2469
                     /locus_tag="VGC82_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY0422424.1"
                     /translation="MFQKILIANRGEIALRIQRACRELGIATVAVHSTADAEAMHVRL
                     ADESVCIGPPPAKDSYLNIPSILSAAAITGADAIHPGYGFLSENAAFADVVDAHGLTF
                     IGPSAAHIGMMGDKIAAKTAMAAVGIPLVPGSDGELPDAETASVVARRIGYPVLIKAA
                     AGGGGRGMKVARDASELEDAYRLARAEARAAFGNDAVYMEKYLDRPRHIELQIMADNH
                     GNVVHFGERDCSLQRRHQKLLEEAGSPAIRSVEREALGKTATAALRRLGYRNAGTLEF
                     LYQDGQFSFIEMNTRLQVEHPVTEMLCDVDLVREQIRIAAGAPLGYDQSDIAFTG"
BASE COUNT          442 a          787 c          792 g          448 t
ORIGIN      
        1 cgatcgccgc gtccgacgcc gtgccgactc cggcatcgac caggaacggc accgtcacat
       61 gctccatcat catggatatc atcgcgggat tctgaatccc caggccggac ccaatcggcg
      121 cacccagcgg catgatcgcc acgcagccca tctcctgtag ccgcttcgcc gcgagcggat
      181 cgtcggcgca ataaaccatc acctcgaacc cgtccttgat cagcgcttcc gctgcctcga
      241 acgtacccgg catgtccggg tacagcagtg gcggtggccc caggacctcc agcttcacca
      301 ggttccagcc gccggcttcc cgagccaggc gcagcgtgcg aaccgcatcc ttggcggtga
      361 agcagccggc ggtattgggc aggtaggtgt actttttggg atcgacgtag tcctgcaaca
      421 tcggagcgcc ggagtccgac aggttcaccc gccgcactgc aacggtgacg atttccgccc
      481 cgctggcctc gatcgcgcgg ccggtctcct ccaggtcctt atagcgcccg gtgccgacga
      541 tcagacgaga ccggaaggtg cgccccgcta cggtccaggt gtcgtcgagt gcgattgatc
      601 cgtccatgct cagcctccac cgatgaaatg cacgatctcc agcgaatcgc cgcttgccag
      661 ccaggtttcg gcgtagcgcg accgcgggac gatctcttcg ttccgctcca ccgcgacctt
      721 cttggtgtcg aggccaagca cgatcaaaag cgccgacacg gacaacgggc catcggtggg
      781 gccttcgaat atccggcttt cgccgttgag ggtcagggtt atggaactcg ccatgcccgt
      841 cagtatggtg cggcggcggc gcggcggcaa tgtttgggga agagaagccg acgtctgccc
      901 accgatgatc gcctccaagc gcatcgcctc ttggccgaga tccgagagca ccgccggctc
      961 ggtcttggaa tggaccccaa gcgtcgctta tgctacgcgc cccggttatg gccgatattc
     1021 tggaggagca gccttgaccc gtattccgat cgatcccgac actatccgtg ctctggcggc
     1081 ggtgctggtg gaaaccggcc tgaccgagat cgaaatcgcc gacaaggaca gccgcatcag
     1141 ggtcgttcgg ggtggcggcg tggccacggc cgtcatgccg gtcatgactc cggctcctgc
     1201 tgttgcggcg cctgccgttc cggacatggc gtcgcatccg ggggcggttc ttagcccgat
     1261 ggtcggtgtg gcgtatctgg cgcctgaacc cggcgctcct gcgttcgtca atgtcggcca
     1321 gcaagtgtcc gccgggcaaa cactgctgtt gatcgaggca atgaagacat tcaatcagat
     1381 caaggcaccg aagtccggca ccgttgccgc gatcctggtg cagagcggcg cgccggtgga
     1441 atacggcgaa gtgctgatcg ttctggaata gtcggttgtt ccagaaaatc ctgatcgcca
     1501 atcgcggcga gatcgccctt cgcattcagc gcgcctgccg ggaattgggg atcgcgacgg
     1561 tggcggtgca ctcgaccgcc gatgccgagg cgatgcacgt ccgcctggcg gacgaaagcg
     1621 tgtgcatcgg cccgcctccg gccaaggaca gctacctcaa tataccgtcg atcctgtccg
     1681 ccgcggcgat caccggggcg gacgcgatcc atcccggata cggcttcctg tcggaaaatg
     1741 ccgccttcgc cgacgtggtg gatgcgcatg ggctgacgtt catcggcccc tccgcggccc
     1801 atatcggcat gatgggcgac aagatcgccg cgaaaacggc gatggccgcc gtgggcattc
     1861 cgctggttcc cggctccgat ggggaactgc cggacgcgga aaccgccagc gtggtcgccc
     1921 ggcgaattgg ttatccggtg ctgatcaagg ccgccgcggg cggtggtgga cgtggcatga
     1981 aagtggcccg cgatgcgtcg gagctggagg atgcctatcg cctggcacgc gcggaagcgc
     2041 gcgcggcatt tggcaacgat gctgtttaca tggaaaaata ccttgatcgg ccgcggcaca
     2101 tcgagctgca gatcatggct gacaatcatg gcaacgtggt gcatttcggc gagcgcgatt
     2161 gcagcctgca gcgacggcat cagaagctgc tggaagaagc cggctcaccg gcgatacgtt
     2221 cagtggagcg tgaagcgttg ggcaaaaccg cgacggcggc gctgcgacgc ctcggctacc
     2281 gcaatgccgg cacgctggag tttttgtatc aggacggaca attttccttc atcgagatga
     2341 atacccggct tcaggtggag catccggtaa ccgagatgct gtgcgatgtc gatctggtcc
     2401 gcgaacagat ccgcatcgcc gccggagcgc cgctgggtta cgaccaaagc gacatcgcgt
     2461 tcactggtc
//
LOCUS       DASWQN010000231         5372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2003323, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000231 DASWQN010000000
VERSION     DASWQN010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003323"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VGC82_03785"
     CDS             complement(<1..200)
                     /locus_tag="VGC82_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422425.1"
                     /translation="MKRFVAPWLLGVGIALTVTACSDPYNPNQRAVGGGLLGAGAGAA
                     IGGLAGGGRAHSPAACWVARSVR"
     gene            complement(291..1700)
                     /locus_tag="VGC82_03790"
     CDS             complement(291..1700)
                     /locus_tag="VGC82_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY0422426.1"
                     /translation="MARLVVVSNRVPLPSERGAKAGGLAVALADALQPGSLWFGWSGR
                     RSARGSNVTHIQRSEGVTYATIDLTEADYHSFYVNFSNGALWPLFHFRLGLVEFRSDD
                     YQGYRSVNRQFAASLVPLLRPDDMIWVHDYHLIPLAAELRALGVTNRIGFFLHTPFVP
                     PTIFHGLPRSTELLTSLCAYNVVGFHTRTYRTAFLDCVRETLGIHPDSEGRFVWKSHA
                     VRAIVDPIGIDAEGFAERAREAARGSDARMLRDSLGKGGLAIGVDRLDYSKGLIQRFE
                     AIGRFFGSYPQHRRQLSFLQIAARSREDQGAYQRLRRELDRIVGDTNGRYSEFDWTPL
                     RYMTRAVKRDTLAGFFRLSRLAVVTPLRDGMNLVAKEYVAAQDPGDPGVLILSRFAGA
                     AEELAEALIVNPYDSDEIAEAMHVGLSMDIEERRDRWNLLHQKVTANTALRFCTVFLN
                     YLGRPDMQPERPPLRAISG"
     gene            1910..2962
                     /locus_tag="VGC82_03795"
     CDS             1910..2962
                     /locus_tag="VGC82_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0422427.1"
                     /translation="MRIAQIAPLTESVPPRTYGGTERVVSYLTEELMAMGHDVTLFAS
                     GDSQTTAKLEAVWPCALRFDTTLRDFMAPQMLLIEKICQQAHNFDVLHCHLDYWPFSV
                     LSRQPTPFLTTLHGRLDLPELTPVYDCFGDAPLVSISDAQRRPLPRANFVSTIHHGLP
                     PDLLTPQPMQRDYLAFLGRICPEKSPDRAIRIARAAGMKLKIAAKIDRCDREYFETTI
                     RPMIDGDQVEMIGEIGEAEKPAFLSGAKALLLPIDWPEPFGLVMIEAMACGTPTIAFP
                     AGSVPEVIDHGLTGYIVHDEAEAVGAVSRLDRLPTAAIRDRFEQRFTARRMAEDYVAL
                     YRRMAVKSRPALRVVT"
     gene            3068..4669
                     /locus_tag="VGC82_03800"
     CDS             3068..4669
                     /locus_tag="VGC82_03800"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY0422428.1"
                     /translation="MGQEYDYIIVGAGSAGCVLANRLSADPNNRVLLLEAGGKDTDPW
                     IHIPAGFYRNIYNPKVAWYYETEPVPQLHNRRISWPRGKVLGGSSSINGLIYIRGQSQ
                     DFDLWRQLGNAGWSYDDVLHYFRKAEDQERGADAFHGSGGPLGVSDLRTDHPLHDAFT
                     AGAQEAGYPYNGDFNGAAQEGVGPLQLTVKGRRRCSAAVGYLNPAKSRPNLRIEVRAL
                     THRVMLKDRRATGIEYSQGGALHHATARKEVLLCGGAINSPQILQLSGIGPGGLLQSL
                     GIEVKHDLPGVGENLQDHIGGRIIHRCVDGTVTMNEIYHNWLRRLYAGAEWILAQKGP
                     LMTGAGPIGLFVKTRPELDSPDVQYQFLAGSAEKTGDPMHRFPGCTMVAIPCRPESRG
                     WLRIQSPDPNKAPAMQPNYLSTQRDRDTIVAGLRVTRRIFATASMQRYCKEEIYPGPK
                     AQTDDELLEHVRATSGTTFHQTSTCMMGPGPKSVVDGDLKVKGLDGLRVIDASIMPTV
                     VSGNTNATVIMIAEKGADMILRAAA"
     gene            4666..>5372
                     /locus_tag="VGC82_03805"
     CDS             4666..>5372
                     /locus_tag="VGC82_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0422429.1"
                     /translation="MTIEQAFAGKVALVTGSGKNIGRTIALDLAARGASVVINGRADR
                     TAVDGVVAEINASGGQAIGCVADVSDPTATQAMIAAAVAAFGGIDILVSNAGLRRQTP
                     FLEMTLAEWREILSVALDGAFILARAAVPHMLGRGGGAIVALSGISTHVGTPNRCHVS
                     ASKAGLEGLMRALAMELAPHGITCNCVAPGAVDTVRGASAGTLPRSQTERGIPLGRKA
                     AMGEISAAVRFLAGPRS"
BASE COUNT         1033 a         1672 c         1771 g          896 t
ORIGIN      
        1 cgcaccgacc gcgccaccca acaggccgcc ggcgagtgcg cccgaccgcc gccggccagc
       61 ccgccaatcg cggcacccgc gccggcaccc agaagtccgc cgccgacggc gcgctgattg
      121 gggttatagg ggtcggagca cgccgtaacc gttagcgcta ttcccacacc cagcagccat
      181 ggcgccacaa atcgcttcat gttgaaacat cctcgttctc gcaacgatga tgttgatatg
      241 gggatgcgat tgtagcggtg tcatcacgaa ctgcggaacc atgatctgaa tcagccggaa
      301 atcgcccgca gcggtggccg ctcgggctgc atgtccgggc gcccaaggta attcaggaac
      361 acggtgcaga agcgcagggc ggtattggcg gtgacttttt ggtggagcaa gttccagcgg
      421 tcgcggcgtt cctcgatatc catcgacagc ccgacatgca tagcctcggc gatctcatcg
      481 gaatcgtagg ggttgacgat cagggcttcg gccagctctt ccgccgcccc ggcgaagcgg
      541 gacagaataa gcacgcccgg gtcgcccgga tcctgggccg cgacatattc cttggcaacg
      601 aggttcatgc cgtcacgcag cggtgtcacg accgcgagcc gggacagacg gaagaatccc
      661 gccagcgtgt ctcgcttaac cgcccgggtc atataccgca gcggggtcca gtcgaactcg
      721 gagtagcggc cgttcgtgtc ccccacgatt cgatccaatt cgcggcggag gcgttgatag
      781 gcgccttggt cctcacgcga ccgcgccgcg atctgcagaa aactgagctg ccgccggtgc
      841 tgcggataac tgccgaaaaa ccggccaatc gcctcgaagc gctggatcag gcccttggaa
      901 tagtccagcc ggtcaacacc gatcgcgagg ccgcccttgc ccaggctgtc gcgcagcatc
      961 ctggcatccg atccgcgcgc ggcttccctg gcgcgttcgg caaaaccctc ggcatcgatc
     1021 ccgatcggat cgacgatcgc gcgtacggca tggcttttcc agacgaaacg tccctcggag
     1081 tcaggatgga ttccgagtgt ttccctgacg cagtcgagga atgcggtgcg gtaggtgcgg
     1141 gtgtgaaacc cgacgacgtt gtaggcacac aggctggtaa gcagttccgt cgatcgcggc
     1201 aggccatgga atatcgttgg cggaacgaac ggcgtgtgca ggaagaagcc gatacggttg
     1261 gtcacaccga gcgcgcgcag ttccgccgcc agtgggatca ggtggtagtc gtgcacccag
     1321 atcatgtcgt ccggccgcag caaaggcacc agcgaggcgg cgaattggcg atttaccgag
     1381 cgataccctt gataatcgtc agagcggaat tcgaccagac cgagccggaa atgaaacaac
     1441 ggccaaaggg cgccgttaga aaaattcacg taaaacgagt gataatcggc ctccgtcagg
     1501 tcgatcgttg catatgtaac gccttcagag cgctggatgt gcgtgacgtt cgatccgcgg
     1561 gcggagcgcc tgccgctcca gccgaaccat agcgaaccgg gctgcagcgc atccgccaaa
     1621 gcgaccgcaa gtccacctgc cttcgcacca cgctccgaag ggagcggcac tctgttagaa
     1681 acgacgacaa gtcgcgccat aggtcagcac ccccggttgc cttcggtcca tttgggcaag
     1741 accacccata accgctttaa cgtaaacgaa gcggcaaagt ggcagtatga ccgtcacatc
     1801 gccgtgagaa tagtaagtag ggcactttga tgaacgccgc cgccgcaaaa agaactcgat
     1861 ccggtcagca acttggatcc cgaagattaa ggccgggagc catttccaaa tgcgtatagc
     1921 ccaaatagca ccgctgaccg aatccgttcc gccgcgcacc tatggcggga ctgaacgggt
     1981 ggtgtcctac ctgaccgagg aactgatggc gatggggcac gacgtgacct tgttcgcgag
     2041 cggcgattcc cagaccacgg cgaagcttga agcggtgtgg ccttgcgcgc tgcgcttcga
     2101 cacgacgctg cgcgatttca tggcgccgca gatgttgctg attgagaaaa tctgtcagca
     2161 ggcacacaat ttcgacgtcc tgcactgcca cctcgactac tggccgttct cagtgctcag
     2221 ccgccagccg acaccgtttt tgacgacctt gcatgggcgg ttggacctgc cggagctgac
     2281 accggtgtat gattgcttcg gggatgcgcc gctggtgtcg atctccgacg cacagcgccg
     2341 gccgctgccg cgggcgaatt tcgtcagcac gatccatcac gggctgccgc cggacctgct
     2401 gacgccgcag ccgatgcagc gcgactatct ggcgttcctc gggcgtatct gcccggagaa
     2461 atcgccggat cgggcgatcc gcatcgcgcg cgccgccggc atgaaactga agatcgccgc
     2521 caagatcgac cgctgcgacc gcgagtattt cgaaacgacc atccggccga tgatcgacgg
     2581 cgaccaggtc gaaatgatcg gcgagatcgg cgaggccgag aaaccggcat tcctgagtgg
     2641 ggcgaaagcg ctgctgctgc cgatcgactg gccggagccg ttcggcctgg tgatgatcga
     2701 ggcgatggcc tgcgggacac cgaccatcgc attcccggcc ggttcggtgc ccgaagtgat
     2761 cgaccatggc ctgaccggtt atatcgttca tgacgaggca gaggccgtcg gcgccgtttc
     2821 ccgcctggat cgcctgccca ccgcggcgat tcgcgaccgg ttcgagcagc gcttcaccgc
     2881 ccggcggatg gcggaggatt atgtggcact gtatcgccgc atggcggtga aatctcgtcc
     2941 ggcgctgcgc gtggtcacct aagccaacca aagccggcta cacaccaaag ccggctacac
     3001 tggtcaagct cgttcggttc cgcttcactg gccgttgcaa caacggccag aacaggcgga
     3061 acgacgcatg gggcaggaat acgactacat catcgtcggc gccgggtcgg ctggctgcgt
     3121 gctggccaac cgcctgagcg ccgacccgaa caaccgggtc ctgctgctgg aggcgggcgg
     3181 caaggacacc gacccgtgga tccacatccc ggcgggattc taccgcaata tctacaaccc
     3241 gaaggtcgcc tggtattatg agacggaacc ggtgccgcag ttgcacaacc gccggatttc
     3301 ctggccgcgc ggcaaggtct tgggtggctc gtcctcgatc aatggcctga tctacatccg
     3361 cggccagagc caggatttcg acctgtggcg ccaactcggc aatgccggtt ggtcctacga
     3421 cgacgtgctg cactacttcc gcaaggccga agaccaggag cgcggcgccg acgccttcca
     3481 tggctccggc ggcccactgg gcgtatcgga ccttagaacc gatcatccgt tgcacgacgc
     3541 gttcaccgcc ggtgcgcaag aggccggcta tccgtacaac ggcgacttca atggcgcggc
     3601 acaagaaggg gttgggccgc tgcaactgac ggtcaaaggc cgccgacgct gttcggccgc
     3661 ggtgggatat ctcaaccccg ccaagtcgcg cccgaacctg cggatcgagg tgcgggccct
     3721 gacgcatcgg gtcatgctga aggatcggcg agccaccggc atcgagtaca gccagggtgg
     3781 cgcgctgcat catgccacgg ccaggaagga ggtgctgctg tgcggcggcg ccatcaactc
     3841 gccgcaaatc ctgcagctct cgggcatcgg gccgggcggg ctgctgcagt cgctggggat
     3901 cgaggtgaag cacgacctgc caggtgtggg ggagaatctt caggaccaca tcggcgggcg
     3961 catcatccac cggtgcgtcg atggcaccgt cacgatgaac gaaatctacc acaactggct
     4021 gcgccggctg tacgcgggtg ccgaatggat cctggcgcaa aaaggcccgc ttatgacggg
     4081 cgccggtccg atcggcctgt tcgtgaaaac ccggccggaa ctggatagcc ccgacgtgca
     4141 ataccagttt ctcgccggca gcgcggagaa aaccggagat ccgatgcatc gcttccctgg
     4201 ttgcaccatg gtggcgatcc cctgccggcc ggagagccgc ggctggttgc gcatccagtc
     4261 gcctgatccg aacaaggcac ccgcgatgca gccgaactac ctgtccacgc aacgggaccg
     4321 cgacacgatc gttgccggat tgcgggtgac gcggcggatt ttcgccacgg ccagcatgca
     4381 gcgctactgc aaggaagaga tttatcccgg ccccaaggcg cagaccgacg acgaactgct
     4441 ggagcatgtc cgagccacga gcgggacgac gttccaccag accagcacct gcatgatggg
     4501 accgggtcca aagtccgtcg tggatggcga tctgaaggtg aaggggctgg acggattgcg
     4561 ggtgatcgat gcgtcgatca tgccgaccgt ggtttccggc aataccaacg ccacggtcat
     4621 catgatagcg gaaaaggggg cagacatgat cctgcgtgcg gcagcatgac gatcgaacag
     4681 gcattcgccg ggaaggtcgc gctggtgacc gggtcgggca agaacatcgg ccgcacgatc
     4741 gcgctggatc tggcggcgcg cggggcgtcg gtggtgatca acgggcgcgc cgaccgaacc
     4801 gcggtggacg gcgtggtggc agagatcaac gcgtccggtg gtcaggcgat cggttgcgtg
     4861 gcggatgtat cggacccaac cgcaacgcag gcgatgatcg cggcggcggt ggctgcgttc
     4921 ggcgggatcg acatcctggt cagcaacgcc gggctgcggc gtcagacccc gttcctcgag
     4981 atgacgctgg cggaatggcg ggagatcctg tcggtcgcgc tggatggggc gttcatcctg
     5041 gcccgtgcgg cggtgccgca tatgctgggc cgagggggcg gggcgatcgt cgcgctgtcg
     5101 gggatttcca cccatgtggg gacgccgaac cgctgccatg tctcggcgtc gaaggccggg
     5161 ttggaggggc ttatgcgggc gctggcaatg gaactcgcgc cgcatgggat tacctgcaac
     5221 tgcgtggcgc cgggtgcggt ggacacggtt cgcggtgctt cggccgggac gctgccgcgg
     5281 tcccagacgg agcgaggcat cccgctcggg cgcaaggcgg cgatggggga aatctcggcg
     5341 gcggtgcgct ttcttgcggg tcccagatcg ga
//
LOCUS       DASWQN010000232         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_288134, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000232 DASWQN010000000
VERSION     DASWQN010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288134"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VGC82_03810"
     CDS             <1..267
                     /locus_tag="VGC82_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018979803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0422430.1"
                     /translation="SFNIYFVMNWRAGQHRPLGSATARAILFSALTTGTAFGSLALSG
                     HPGTASMGKLLLISLGCTLLASLIFIPALLASLKTPVSNRSERL"
     gene            267..599
                     /locus_tag="VGC82_03815"
     CDS             267..599
                     /locus_tag="VGC82_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422431.1"
                     /translation="MGGRLPAAVALLFLAAAAAPPGDCGLVGDPGPVLPLPLDLAGRP
                     GMAAGLAGHGFAALPGAGASFDCREVVPNAQQPGTLRSESSDILHGLPAPDILRPINE
                     PRRAPQFQ"
     gene            689..838
                     /locus_tag="VGC82_03820"
     CDS             689..838
                     /locus_tag="VGC82_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422432.1"
                     /translation="MKKLLLVALIPLGLGGCLSFQGSPPPAKNTIVVPPGSTVSCSSG
                     QPGPC"
     gene            complement(1064..2038)
                     /gene="speB"
                     /locus_tag="VGC82_03825"
     CDS             complement(1064..2038)
                     /gene="speB"
                     /locus_tag="VGC82_03825"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY0422433.1"
                     /translation="MIKQPEKYQDFHDPQALPRYTGIASFFRAPASQQMDNVDIGIIG
                     VPFDGGVTNRSGTRHGPRAVREQSTLLRRINAVTGIMPFATARVRDLGDCWIEQPYEL
                     LGALKEIETFCRTVTAAGIVPLSVGGDHSISLPILRAVAANGPVGMIHVDAHCDTGDD
                     YMGSRFHHGAPFRRAVEEGLLDPKRVIQIGIRGTTNDPDMWGFSQRSGMRVLPMEEFD
                     DKGWRYAAEEARRVAGHGPTYLTFDIDSLDPSQAPGTGTPEAGGLTALQALRLLRELR
                     GIDFVGGDLVEVSPPFDVGSVTAFNGASVLFEILCLLAEAREVREVSR"
BASE COUNT          382 a          739 c          727 g          439 t
ORIGIN      
        1 tcgttcaaca tctatttcgt gatgaactgg cgcgccggac agcatcgtcc gctgggaagt
       61 gctacggcgc gggctatctt gttttccgcg ttgacgaccg gcacggcgtt cggatcgctg
      121 gctttgtcag gccacccggg aacggcgagc atgggaaaac tactgctgat cagcctgggc
      181 tgcacgctgc tggccagcct gattttcatt ccggcgttgc tcgccagcct gaaaacgcct
      241 gtcagcaacc ggtccgagag gctctgatgg gcgggagatt gcctgctgct gttgcactgc
      301 tgtttttagc ggccgctgcg gctccccccg gcgactgtgg tttggtcggc gatcccggac
      361 ctgttctgcc attgccgctc gatctggcgg ggcgccctgg tatggcggcc gggctggctg
      421 ggcatggttt cgccgcgttg cccggtgcgg gggcctcgtt cgactgccgc gaggtggttc
      481 cgaacgcgca gcagcctggc acgttgcgca gcgagtccag tgatattctg cacggccttc
      541 cggcacccga catcctgcgg ccgatcaacg agccgcggcg ggcaccgcaa ttccagtagt
      601 acgcacggtc aattgacgcc gcgtccggtt cctgaagctc gggaccgaaa ccatttgagt
      661 actgtgctgt cataagagga tttatcacat gaagaaactg ctgcttgtcg ctttgatccc
      721 gcttggtctc ggcggctgcc tcagcttcca gggaagcccg ccgccggcca agaacaccat
      781 cgtcgtgccg ccgggatcga ctgtgagctg ctcgagcggc caacccggcc cctgctgaag
      841 cccggcgcca gctgaagccc ggtccctgct gaaggctggt cccggcaaac gcgcagccgg
      901 gcagcggttt gacttgatca gagtgtcgtg gccgtgctcg ttgcggtcac ccggcataac
      961 gtgatgggtt cttggcgcgg ccacccacga cttttcacgc gcgggaaaca gcgggtggca
     1021 aggataaaca gcgggtggca gggataacgt cgaccctgcg caactatctg gacacttcgc
     1081 ggacctcccg cgcttccgca agaagacaca ggatttcgaa cagaaccgat gcaccgttga
     1141 aggccgtcac ggagccgaca tcgaacggcg gcgaaacctc caccagatcg ccgccgacga
     1201 agtcgatgcc acgcagctcc cgcagcagtc gcagcgcctg caaagccgta aggccgccgg
     1261 cctcgggggt tcctgtcccc ggagcctggc tgggatcgag gctgtcgata tcgaaggtca
     1321 ggtaggtggg gccatggccc gccacgcgcc gagcttcctc ggccgcataa cgccacccct
     1381 tgtcgtcgaa ttcttccatc ggcaggaccc gcatgccgct gcgctggcta aaaccccaca
     1441 tgtccggatc gttggtcgtg ccgcggatgc cgatctggat cacgcgcttc gggtccagca
     1501 gaccttcctc cacggctcgt cggaacggcg cgccgtggtg aaagcgcgat cccatgtaat
     1561 cgtcgccggt gtcgcaatgg gcatcgacat ggatcatgcc gacaggcccg ttggctgcca
     1621 cggcgcgcag gatcggcagg ctgatggaat ggtcgccgcc gaccgacagc ggcacgatcc
     1681 cagcggccgt gaccgtgcgg cagaacgttt cgatctcctt cagtgcgccc agcagctcat
     1741 atggctgctc gatccagcag tcccccaggt cgcgcacgcg cgccgtcgcg aacggcatga
     1801 tcccggtgac ggcattgatg cggcgcagca aggttgactg ttcgcgcacc gcccgggggc
     1861 catgcctggt gccggaacgg ttggtgacac cgccgtcgaa cggcacgccg atgatgccga
     1921 tatccacatt gtccatttgt tgcgatgccg gagcgcggaa gaagctcgcg atgccggtgt
     1981 accgaggaag cgcctgcgga tcatggaaat cctggtattt ttctggctgc ttgatcatcc
     2041 gtctcgctct tcagtacgtg atgtcgtgca tgttgccctg tctcgcgagg ttttccccct
     2101 cgattcgatg ccgtccgcgc aaatccggat cttggtgttc gccgcgcgcg agccgcctta
     2161 aaccggtccc gctgttattc agccgtggcc ccaggccgcg gccgctccgg catcggcatc
     2221 gattcgcgtt caaaaacctg ggcatcccca gcggcggccg aaccagtgga tatcgaacga
     2281 gcgggat
//
LOCUS       DASWQN010000233         5548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2267967, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000233 DASWQN010000000
VERSION     DASWQN010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267967"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VGC82_03830"
     CDS             complement(<1..246)
                     /locus_tag="VGC82_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422434.1"
                     /translation="MWLFMVPSILVMTIVLLYPLGSAIYFSFLRFYLDGGTARFIGLD
                     NYQALLTDERFWGDLATTFVIVICSVAAQFIIGMTVAL"
     gene            complement(260..1507)
                     /locus_tag="VGC82_03835"
     CDS             complement(260..1507)
                     /locus_tag="VGC82_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0422435.1"
                     /translation="MTGSTITRRGAIGAAVALGLPSIRRSYADAPVSLISHRYPGLEY
                     YAEKIKTALPGVPVDARLMQAPEAIQLSRISLSARSNQMDLVWANNIVLASFAKSGWL
                     EPLDDLWAKHAEEFNLNDISPTLVKSCMFGGHIYGMPLTVNTTVYAYRADLYEQKGLT
                     PATTWDGYIENAKALTTPPRRYGATIALKSDMVANEMNAVMNTVGDGWFDKDWRPTFN
                     SPRGIEAVETYKRLAKYAVPGFTAVHNDEHTVNMGQDIAAQGQQWATRCSSMDNPEKS
                     RVVGKIKWTVMPSGGRQVGVSDIYCISRYSVKDKDTLFRIIATALREENQRGAAALAV
                     PTRQAVVKDPALAEKYRWYPAVSQALAIAQPMPSLPEFSEASEVIYKRIVQAIVGQME
                     TKAALDAAATEVQAMLAERGYYK"
     gene            1906..2169
                     /locus_tag="VGC82_03840"
     CDS             1906..2169
                     /locus_tag="VGC82_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422436.1"
                     /translation="MAVDEIDLMLQRGQVPAGMTDRHGYSDFTDPLGKYLDLLAAGAF
                     AEAKPIQQDACKTWLATRRDSAMTGKFWIWNTEINLDTLCAGR"
     gene            2604..4331
                     /locus_tag="VGC82_03845"
     CDS             2604..4331
                     /locus_tag="VGC82_03845"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding and patatin-like
                     phospholipase domain-containing protein"
                     /protein_id="HEY0422437.1"
                     /translation="MADRTRNLRRAFPLADAASIEALSSICSLMSVPGGVALVLEGEH
                     PEAVYVVVTGLLGAYQPAADGQDVLLDRFGAGDLIGDTGFITGETRTVTIRALRNSEL
                     LRIPEQDLRAASVACPGILQALCSAVVQRLQRTQPASASPLKCRTFCLVPADTSIDMK
                     PIIQKIAATLEAFGSVATIAAAPSSERTSAWFAEVEKTSDFVLLEAECDLTAWTRFCL
                     RQCDRIVLLAHGETEPADCEVFGPGGAGVPHKTPVSLMLLWRHEVVPTRTANWLQSVN
                     PNGHHHIRTSADIARASRLMAGRGLGLVLSGGGAKALAHVGVIEALRDQGIEIDAIGG
                     TSIGAIVAAVFAQEWDLGDTVRTLAAAFNRRRFSDFAVPRTALYSERAFARTLGRCFK
                     DMVIEDSPIPLFCVSTNLTAGLSAVHRTGRLLTWLRATSAVPGICPPVVEQNAVYVDG
                     GVLNNLPIDAIRDFGVASVIAVDVGSSLQAQARSDGTNGLPNIVDLLWRVGSIGSDAA
                     SRIRRDGDVVLKPEVGSLGLFDWNAHEVAIAAGHQVVLDHLDEIEAAVSRTLDAKRSE
                     PTLATAAAG"
     gene            4516..>5548
                     /locus_tag="VGC82_03850"
     CDS             4516..>5548
                     /locus_tag="VGC82_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0422438.1"
                     /translation="MINDLWYKNAIIYCLSVGTFMDANGDGVGDFAGLMRRLDYLQGL
                     GVTAIWLMPFQPSPMRDDGYDITDYYGVDPRYGTLGDFVEFAHGCKQRGIRVLIDLVV
                     NHTSDQHPWFKEARRDAQSPYRDWYVWADHEPPGAETGVVFPGVQLSTWDYDDTAKAW
                     YFHRFYDFQPDLNTSNPRVQTEILKIMGFWLQLGVSGFRMDAVPFVIAKKGADQTEAV
                     EQYDMLRGFREFLQWREGDAVILAEANVLPDTDLKYFGDDGDRLHMMFNFQVNQNLFY
                     ALAAADTQPLIAALEATRGKPATAQWGNFLRNHDELDLGRLSKEQRQAVFAEFGPEPD
                     MQLYGRGIRR"
BASE COUNT         1020 a         1667 c         1713 g         1148 t
ORIGIN      
        1 gagcgcaacg gtcatgccga taatgaattg tgccgcgacg gagcagatga cgatcacgaa
       61 tgtggtcgcg aggtcgcccc aaaatcgctc atcggtcaac agggcctggt agttgtccag
      121 gccgatgaac cgggcggtgc ctccgtccag gtagaaccgg aggaaactaa agtatattgc
      181 agacccaagc gggtaaagca gcacaatggt catgaccaaa atgctgggaa ccatgaacag
      241 ccacatgacg ctgttccggt tacttgtagt atcctcgttc ggcaagcatc gcctgaactt
      301 cggtggcggc ggcgtcgagc gcggctttcg tttccatctg gccgacgatc gcttgtacga
      361 tgcgcttgta gatcacctcg gatgcctcgg aaaactccgg cagggacggc atcggctgtg
      421 cgatcgcgag cgcctgggat accgcgggat accagcggta tttttccgct aaagccgggt
      481 ccttcaccac ggcctgacgg gtcggcactg ccagcgccgc cgcgccacgc tggttttcct
      541 cccgcagtgc ggtagcgatg atgcgaaaca gggtatcctt gtccttaacg ctatagcggg
      601 agatgcagta aatgtcgctg acccccacct gacgtccgcc ggacggcatg acggtccatt
      661 tgatcttgcc gacgacgcgc gatttctcag gattgtccat gctcgagcag cgcgttgccc
      721 attgttgtcc ttgcgcggcg atgtcctggc ccatgttgac ggtgtgctcg tcattatgca
      781 cggcggtgaa tcccggcacc gcgtatttcg ccagccgctt ataggtctcc accgcctcga
      841 tgccgcgcgg gctgttgaag gtcggccgcc agtccttgtc gaaccagcca tcgcccacgg
      901 tgttcatcac cgcgttcatt tcgttcgcta ccatgtccga tttcagcgcg atcgtcgcgc
      961 cgtagcggcg cggcggagtg gtcagcgcct tcgcgttctc gatatatccg tcccaggtcg
     1021 tggccggggt aagacccttc tgctcataca aatcggcacg ataggcatac accgtcgtgt
     1081 tcacggtcaa aggcatgccg taaatatggc cgccgaacat gcagcttttc accagcgtcg
     1141 gcgaaatgtc gttcaggttg aactcttccg cgtgcttggc ccaaagatcg tcgagcggct
     1201 ccagccagcc gcttttggca aaggaagcca ggacgatgtt gttggcccac accaggtcca
     1261 tttggttgga ccgggcggac agcgagatgc gggaaagctg aatggcctcg ggcgcctgca
     1321 tcagtctggc gtccaccggc accccgggca gcgcggtttt gatcttttcg gcgtaatact
     1381 ccaggccggg gtagcggtgg ctgatcagcg acaccggcgc gtccgcgtag gaacggcgga
     1441 ttgaaggcag gccaagggcg accgcggcac cgatcgcgcc acgccgcgta atggtgcttc
     1501 cggtcatggc gtctttctcc ccaccgtctc acggtgatat ctgttgttct ggcaaggtct
     1561 gttgtcctgg tgaggagcgt cgcacggcga aaccgtgtcg tcgagacacc gcctgcagaa
     1621 ggctcgaacc tgggccggga tcagttgccg gatgcctcgc atccggtccg gcggacatcg
     1681 gtgcagctgg cccggcgaca cgggtgtcac catttccagc caaccgcgtg ggctgtagtg
     1741 tcgtccgcta tgatgccggc cagaccgccc ggtggtcttc gtcaccgccc ggtggtcttc
     1801 gtcggcatcg gtcccgccaa cggaggactt gccgtgcaac aaccgcctgt ccgatgcgcg
     1861 attgcacttg ccgccgcggc cgctctgctt tgtggcgaat cagccatggc cgtggacgaa
     1921 atcgacctga tgttgcagcg tggccaggtg cccgccggta tgaccgaccg gcatggatat
     1981 tcggacttca ccgatccctt ggggaagtac ttggacctcc tggcggcggg ggcgttcgcg
     2041 gaagcaaagc cgatccagca ggatgcctgc aagacctggt tggcgacgcg gcgggattcg
     2101 gccatgaccg gcaagttctg gatctggaat actgagatca acctcgatac gctctgtgcc
     2161 ggacgctaga tcattgcgga ttgaccgaca gcgcaaactc gctgtaggcg gtcaagcttg
     2221 accgcgccct ctcgtggccg tcatcggggc gcgaccagcg ccgggcgcgg attttgcaac
     2281 gatcgggcgg cgagcgtcag tgcgggcagc acgagatttt gacctgcatt aaccgaattc
     2341 cactccagca taacgaccta ttgcgatggc cggcgcattt tggccatcat attccttaag
     2401 ctgacaggat ggaggcggat acgctgcctc accgatatga agacaacagg ggggtcccgg
     2461 tgccggaaag gatcattcgc ctgttgagta gccttagacc gcttacgcag gggcctggcg
     2521 actggttttc ccgttcgtgc gatgtcaacc gcactccatc cggcaacaaa cggctcaatg
     2581 agccagaacg atccgaccca agtttggccg accgaactcg gaacctgcgg cgcgcgtttc
     2641 cgctggcgga tgccgcgtcg atcgaggcgt tgagttcgat ctgctcgctg atgtcggttc
     2701 ccggaggtgt cgccctggtc ctggagggcg agcaccccga ggcagtctac gtcgtcgtca
     2761 cgggactatt gggtgcctat cagccggcag cagatggtca ggacgttctc cttgatcgat
     2821 tcggtgccgg cgatttgatt ggcgacactg ggttcataac cggtgaaacc cggacggtta
     2881 ccataagggc gttgcggaac agcgagcttt tgcggatacc ggaacaggat cttcgcgccg
     2941 cttccgttgc ctgcccgggg atcttgcagg cgctctgttc agccgtggta caacggcttc
     3001 agcgcaccca gccagcatcg gcatctccgc taaaatgccg cacgttctgt ctggtaccgg
     3061 ctgacacgag catcgacatg aagccgatca ttcagaagat tgccgctact ttagaagcct
     3121 ttggcagcgt tgccacgata gcggcggcac catcgtcgga acgaacatcg gcatggttcg
     3181 ctgaagtcga aaaaacttcg gattttgtcc tcctcgaagc cgaatgcgat ctgactgctt
     3241 ggacccggtt ttgcctgcgg caatgcgacc gcatcgtgct gttggctcat ggtgaaaccg
     3301 agcccgctga ctgtgaagtc tttggcccgg gtggtgccgg cgtcccgcac aagacgcccg
     3361 tgtccttgat gctgctttgg cgacacgagg tcgtgcccac gagaacggcc aattggctgc
     3421 agtctgtgaa cccgaacggg caccaccaca tccgcacgtc cgcggatatc gcaagggcaa
     3481 gccgcttgat ggcgggccgc ggtctcgggc tggttctgtc gggtggcgga gccaaggctc
     3541 tggctcatgt cggtgtcatc gaagctttgc gagaccaggg gattgagatt gacgccatcg
     3601 gcgggaccag cattggcgcc atcgtagcgg ccgtgttcgc gcaggagtgg gaccttggcg
     3661 ataccgtccg aactcttgcc gctgctttta accgccggcg cttttcggat ttcgcggtcc
     3721 ctcgaacggc cctgtattcg gagcgggcgt tcgcccggac cctcggccgc tgcttcaagg
     3781 acatggttat cgaggattca ccgataccgc tgttctgcgt ttcgacgaac ctgaccgccg
     3841 gcctatcggc cgttcatcgg acgggccgac tcttgacctg gctccgtgcc acatccgcgg
     3901 ttcccggcat ttgccccccg gtcgtggaac agaacgcggt ttacgtcgat ggcggcgttc
     3961 tcaacaacct gccgatcgat gctatccgcg actttggtgt agcgtcggtc attgccgtgg
     4021 acgtcggctc ctcgctacag gcgcaagctc ggtcagatgg caccaacggc ctgccgaaca
     4081 tcgtggatct gctctggcgt gtcggaagca tcggaagcga cgcagcttcc cgcatacggc
     4141 gggacggcga cgtggtactc aagcccgagg tgggaagcct tggattgttt gattggaacg
     4201 cacacgaggt ggcgatcgcc gcgggccatc aggtcgtcct cgaccatctc gatgagatcg
     4261 aggccgctgt cagccgtacg cttgacgcaa aacgatcgga acccacgctc gcaacggctg
     4321 ctgccggcta atgccatgtc cgatagggga acggcccctg agtgatcggg gccggctctg
     4381 ctgaagcatt ttcagcctga ctgtgcgggt cgaaaatgct ctatccgctt gtttgctcgc
     4441 gtgattcaca cgtttcagac gttccgccca gcgtgatcac cctctacggt agcgtctaag
     4501 agcagagcgg acatcatgat caacgatctt tggtacaaaa atgcgatcat ctactgcctg
     4561 tcggtgggca ctttcatgga tgccaacggc gacggcgtcg gcgacttcgc cggcctcatg
     4621 cgccgactgg attatcttca aggcctgggc gttaccgcga tctggctgat gccgttccag
     4681 ccatcgccca tgcgtgacga cggttatgac atcaccgatt attatggcgt cgatcctcga
     4741 tacggcacgc tcggagactt cgtcgagttt gctcatggtt gcaagcagcg cggcatccgc
     4801 gtgctgatcg acctggtggt gaaccacacc tccgatcagc acccttggtt caaggaagcc
     4861 cgacgcgatg cacagtcgcc atatcgcgac tggtacgtct gggcggacca cgagccgccc
     4921 ggggcggaaa caggcgtggt gtttccaggc gtgcagttat cgacctggga ctacgacgac
     4981 accgccaagg cttggtattt tcaccgcttc tacgattttc aacctgacct caacacatcc
     5041 aacccgcgcg tccaaacaga gatcctgaag atcatggggt tctggctcca gcttggcgtg
     5101 tcgggcttcc gcatggacgc cgttccgttc gtgatcgcga agaagggtgc ggatcaaacc
     5161 gaggctgtcg aacaatacga catgttgcgt ggcttccgcg aattcctgca gtggcgggag
     5221 ggcgacgcgg ttattctggc ggaagccaac gtcctgccgg acacggatct gaaatacttc
     5281 ggcgatgacg gtgaccggct tcacatgatg ttcaacttcc aggtcaatca gaacctgttc
     5341 tacgccttgg cggctgccga cacgcagcca ctgatcgcgg cgctggaggc tacgcgcggc
     5401 aagccggcca ccgctcaatg gggaaatttc ctgcgcaatc atgacgagct cgatcttggc
     5461 cggctgagca aggagcagcg tcaggccgtg ttcgcggaat tcggacccga accggatatg
     5521 cagctttacg ggcgcggaat ccggcggc
//
LOCUS       DASWQN010000234         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1872296, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000234 DASWQN010000000
VERSION     DASWQN010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872296"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VGC82_03855"
     CDS             complement(<1..600)
                     /locus_tag="VGC82_03855"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY0422439.1"
                     /translation="MSQEPPEKMMFQLNLRRRGISDQAVLRAMEEVPREVFVAAADRG
                     QAYRDSALGIACGQTISQPFVVAYMTEQLQLQKRHKVLEIGTGSGYQAAVLSILCRQV
                     VTIERFRTLADTARKRLKQLHCDNVEVKLGDGFDIPAGAGQFDRIIVTAAMEQIPDAL
                     SGRLKPGGILIAPVGPHHGVQTLVRVVRTEAGFELKELVD"
     gene            845..1222
                     /locus_tag="VGC82_03860"
     CDS             845..1222
                     /locus_tag="VGC82_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0422440.1"
                     /translation="MMATRPVGCSVFLVEDEVMIRMMVADMLEELGYTIAAEAGEIGE
                     ALRLVPSTDFDLAILDVNVNGKVITPVAELIRAKGRPFIFATGYGSSGLPEEYRDRPA
                     LQKPFQLETLAQVIDSTLKSAAV"
     gene            complement(1346..>1722)
                     /locus_tag="VGC82_03865"
     CDS             complement(1346..>1722)
                     /locus_tag="VGC82_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY0422441.1"
                     /translation="TRHAPLWDTALKFGPDILRKVIDAGIPKNTVINVNFPACTPDLV
                     KGVRVTRQGKRNVGFLKVDKRHDGRGNPYFWLGFERAAALEVPAEGTDLAALVAKYVS
                     VTPLRLDRTDEAFSDALTTTLK"
BASE COUNT          310 a          545 c          544 g          323 t
ORIGIN      
        1 gtccaccaat tccttgagct cgaaccccgc ttcggtcctg accactcgga ccagggtctg
       61 cacgccgtga tgcggaccga cgggcgcaat aaggatgccg ccgggcttga gccggcctga
      121 cagcgcatcg gggatctgct ccatcgccgc ggtgacgata atgcggtcga actggccggc
      181 gcccgccggg atatcgaagc cgtccccgag cttgacctcg acgttgtcgc agtgcaactg
      241 cttcagccgc ttgcgggcgg tgtcggccag ggtgcggaag cgttcgatgg tcacaacctg
      301 ccggcaaagt attgacagga ccgcggcctg gtagccggag ccggtgccga tctcgagcac
      361 cttgtggcgc ttttgcagct gcaactgctc ggtcatgtag gcgaccacga agggctgact
      421 gatggtctgc ccgcaggcga tgccgagcgc gctgtcccgg taggcctggc cgcgatcggc
      481 cgccgcgacg aacacttccc gcggcacctc ttccatggcg cgcagcacgg cttgatcgct
      541 gatcccccgc cgccgcaggt tgagctgaaa catcatcttt tcgggcggtt cttgcgacat
      601 tgcgctctcg tggcgggcca gacccgccaa tcctatgctt ccgccacacc gagatgtact
      661 aacggcgacg gatttaagaa gaatttcctg aaaaattcgg ttcccggccc gggaacccta
      721 actcccccgg ggcgttggaa caccggaaaa tcgtaggact gccggttccg gcaggctaag
      781 gccggcgttg caccggggag tgttttttgc gaaccttggc gcccggaagg gcaaggattc
      841 catcatgatg gcgacgcggc ccgtgggctg ttctgttttt ctcgtcgagg acgaggtcat
      901 gatccggatg atggtggccg acatgctcga agagctgggc tacaccattg cggccgaagc
      961 cggcgaaatc ggcgaggcgc tcaggctggt gccatcgacc gatttcgacc tcgccattct
     1021 cgacgtcaac gtcaacggca aggtgatcac gccggtggcc gaactgatca gggcgaaagg
     1081 ccgcccgttc attttcgcga ccggctacgg gtcatccggc ctgcccgagg aataccgcga
     1141 ccgtccggct ttgcagaagc cgttccagct ggaaacgctg gcccaggtca tcgacagcac
     1201 gctgaagagc gcagcggttt agcgcggcct ccccacgtct gtcatcatcc gcgaaagcgg
     1261 atgatccagt atccgaaaga ccatcgactg agactcaaag ccggggcgta ctggataccc
     1321 gcatgcgcgg gtatgacgct tcgcgttact tcagcgtcgt cgtgagcgcg tccgaaaacg
     1381 cttcatccgt gcgatcgagc cgcagcggcg ttaccgacac atacttggcc accagtgctg
     1441 cgaggtcggt gccttcggcc ggcacctcca gcgccgcggc gcgttcgaag ccgagccaga
     1501 aatacggatt gccgcggccg tcatggcgct tgtcgacttt gaggaagccg acgttgcgct
     1561 tgccctgccg tgtgacgcga acgcccttga ccaggtccgg cgtgcaggcc gggaaattga
     1621 cgttgatcac ggtgttcttc gggatgccgg cgtcgatcac tttgcgcaaa atatccggcc
     1681 cgaatttcag cgcggtatcc cacagcggcg catgacgcgt ct
//
LOCUS       DASWQN010000235         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_552674, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000235 DASWQN010000000
VERSION     DASWQN010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552674"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VGC82_03870"
     CDS             <1..1195
                     /locus_tag="VGC82_03870"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEY0422442.1"
                     /translation="RNILGGEFSYKTNFTSLTRDTAAFDPITTSANTQGLCTMASADP
                     LARMPNQCLLRGMPGTYTRLTGEAQWRRSFTDSAGEIWTPFASIRADAIDASISNQPG
                     VSNFLPVGDTQALRVMPTVGLEYRYPFINVQPWGTTTIEPIAQVIIRPNETYAGKLPN
                     EDAQSLVFDTSNLFSVDKFSGYDRVEGGGRANVGVRATTQFDRGGSVTAVFGQSYQLF
                     GLNSFAVADVTNTGVDSGLATPKSDYVGSINYSPNRTYTFSVRSRFDQATVNVQRFEA
                     EARANFDRWSVSMMYGNYAAQPEIGYLTRREGLLGSASVKVAANWVVSGSARWDLVAN
                     QINQYVIGAGYVDDCFVLAANYVTSFNYSTATTPPVLSHAFMLQIGLRTLATTAASGG
                     AGAIQ"
     gene            1280..>2136
                     /locus_tag="VGC82_03875"
     CDS             1280..>2136
                     /locus_tag="VGC82_03875"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY0422443.1"
                     /translation="MTTTFSHRLSPFLLGCAAALLLIGCGSPLHAQTVAVMVNGEPIT
                     NYDIEQRSKLTFLTTHKPADRQQVINELIDEKVKIKEGKKFGVDPSISDIDQSYAAMS
                     SRMRITPEQLTKSLESQGIRPDTLKARMKAEMVWTSLIRGRYKESLQVGEKEVAAAVH
                     VNGDEKQETESFEYKMQPIVLIVPRGSAPAAVEARHKEAEALRSRVQTCDEANAFFKS
                     MQNAAIREAVTKTSADIPQVLREVLDKTPIGHLTAPELTKQGVEMVALCGRKPTTIDT
                     PKKKEIRDK"
BASE COUNT          474 a          670 c          613 g          379 t
ORIGIN      
        1 ccgcaatatc ctgggcggcg aattcagcta caagacgaat ttcacgagcc tgactcgaga
       61 cacggccgcg ttcgacccga ttacgacgtc ggcgaacacc cagggcctgt gtacgatggc
      121 gtccgccgat cctctggcgc ggatgccgaa ccaatgtctg ctccgcggca tgcccggcac
      181 ttacacgcga ttgacgggtg aagcgcaatg gcggcgatca ttcaccgatt ccgcgggaga
      241 aatctggacg ccatttgcga gcatccgagc cgatgcgatc gatgcctcga tctcgaacca
      301 gccgggcgtt tcgaacttcc tcccggtcgg cgacacgcag gccctgcgcg tgatgcccac
      361 ggtcggcctc gaatatcgct atcccttcat caacgttcag ccctggggca ccacgacgat
      421 cgagccgatc gcacaggtca tcattcgccc gaatgaaacc tatgccggca agcttcccaa
      481 cgaagacgcg caaagtctgg tgttcgacac cagcaatttg ttcagcgtcg acaagttctc
      541 cggctatgac cgcgtcgagg gcggcggccg cgccaatgtc ggcgtgaggg cgaccacgca
      601 attcgaccgc ggcggctccg tcactgcggt gttcggccaa tcctatcaac tcttcggcct
      661 gaactcattc gcggtcgcgg acgtgaccaa taccggtgtc gattccggtc ttgcgacccc
      721 aaaatccgat tatgttggca gcatcaatta ttcgcccaac agaacctata ccttcagcgt
      781 acgctcgcgc ttcgatcagg cgacggttaa cgtccagcgt ttcgaggctg aagcccgcgc
      841 gaacttcgac cgctggtcgg tcagcatgat gtacggcaat tatgccgcgc aaccggaaat
      901 cggatacctg acccgccgag aaggattgct gggcagcgcg tcggtcaagg tggcagcgaa
      961 ctgggtggtg tcgggatctg cccggtggga tctggtagcg aatcagatca accaatatgt
     1021 catcggcgcc ggctatgtgg acgactgctt cgtgctggcg gccaactatg tcacatcctt
     1081 caactattcc acggccacca cgccgcccgt gctcagtcac gcttttatgc tgcagatcgg
     1141 cctgcggacc cttgccacga cggccgcaag tggcggagcc ggggctatcc agtagaaccg
     1201 gagccggcac gctgccggag cacgaactcc cggcgcggcg cggctccaat ccgaatcggt
     1261 tgatatagcg aatatgatca tgacgaccac attctctcac cggctttcgc ccttcctcct
     1321 gggttgtgcc gcggcactgc tcttgatcgg ttgcggttcg ccgttgcatg cgcaaaccgt
     1381 cgccgtcatg gtcaacggcg aacccatcac caactacgat atcgaacagc gcagcaagct
     1441 gactttcctg acgacccaca agcctgccga ccgacagcaa gtgatcaacg aattgatcga
     1501 tgaaaaggtg aagatcaagg aaggcaagaa gtttggcgtc gaccccagca tttccgatat
     1561 cgaccagtct tacgcggcga tgagttcgcg catgcgaatc acgccggagc agctgacaaa
     1621 atctctggaa agtcagggca tccggccaga cacgctgaag gcccgtatga aggccgagat
     1681 ggtttggacc agcctgatcc gcggacgata caaggagagc ctccaggtcg gcgaaaagga
     1741 agtcgccgcc gccgtccacg tcaacggcga cgagaagcag gagaccgaga gcttcgaata
     1801 taagatgcag ccgatcgttt tgatcgtccc gcgaggatcc gcgccagccg ccgttgaagc
     1861 aagacacaag gaggccgaag ccctgcgcag ccgcgtgcag acctgcgacg aggcaaacgc
     1921 tttcttcaag tccatgcaaa atgccgcgat acgcgaggcc gtgaccaaga cctcggccga
     1981 cataccgcag gttctccgcg aggtcctcga caagactccg atcggccact tgaccgcgcc
     2041 tgagctgacc aagcagggcg tcgagatggt ggcgctgtgc ggacgcaagc cgacaaccat
     2101 cgacaccccg aagaaaaaag aaatccgcga caagat
//
LOCUS       DASWQN010000236         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_552795, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000236 DASWQN010000000
VERSION     DASWQN010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552795"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VGC82_03880"
     CDS             <1..1186
                     /locus_tag="VGC82_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422444.1"
                     /translation="PDAKTIVLTLKEPYGLVLDSIAKPSSLVPFMMPKRMAETPPGQQ
                     IKEQIGSGPFKFVQAEFQPGVKAVYEKNKDYVPRKEAPSWTSGGKVVKVDRVEWLTMP
                     DAQTAVNALQSGDIDFMENPSWDILPVLKANPDLKVEALDKVGFQTVGRMNFLHPPFD
                     NVKVRRAAFMAMKQKDVLDAMVGNPEYYRICGAVFVCGTPLATDVGSESLVKGNGMAE
                     ARKLLAESGYDGAPIVIMAPGDVVTLKAQPVVAAQLLREAGFKVDLQATDWQTVVARR
                     ASQKPPKEGGWNMFFTNWSGADVGNPVANVSVGGRGTKGGWFGWAEDAKIEAMRDAFA
                     RSSSPEEQKKIAADIQKENYDQVIYIPLGQYLAPSAWRKSLSGVLEGPATPIFWNIDK
                     SE"
     gene            1834..>2696
                     /locus_tag="VGC82_03885"
     CDS             1834..>2696
                     /locus_tag="VGC82_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422445.1"
                     /translation="MLSQSHICSQTPSGATLASGGATFRVWAPGATAVYLHGNFGGQV
                     FDQLTPDRLLQKNARGYWTGFQDGARDGDRYRFWVQGAGSSGYKRDPYARELEPEGFP
                     NCFSIVRASSFPWHDQDFVTPDFSDMIIYQLHIGTFAIRKPGVPSNLLDVALKVPYLA
                     ELGINVLQPLPVDEQESNPSMGYSGADLFSPDFPYIAVEDLPFYLEKLNQFYAAKQKA
                     AITLADIQSAPGQLKALVDLCHVNGIAVVFDVVYNHAGGFSVNGQFDDHCLYYMDRRP
                     NRGNNNDSLYFT"
BASE COUNT          510 a          848 c          866 g          472 t
ORIGIN      
        1 gcccgacgcc aagaccatcg tgctgaccct gaaggagccc tacggcctgg tgctcgactc
       61 gatcgcgaag ccgtcgtcgc tggtgccgtt catgatgccg aagcggatgg cagaaacgcc
      121 tcccggccag cagatcaagg aacagatcgg ctccggcccg ttcaagttcg tgcaggccga
      181 attccagccg ggcgtgaagg ccgtctatga gaagaacaag gactatgtgc cgcgcaagga
      241 agcgccaagc tggacctccg gcggcaaggt ggtgaaggtc gaccgcgtcg aatggctcac
      301 gatgccagat gcacagaccg cggtgaatgc gctgcagtcc ggcgacatcg atttcatgga
      361 aaacccgtcc tgggatatct tgccggtcct caaggccaac ccggacctga aggtcgaggc
      421 gctggacaag gtcggcttcc agacggtcgg gcgcatgaac ttccttcatc ccccgttcga
      481 caacgtcaag gtgcgccgcg ccgccttcat ggcgatgaag cagaaggacg tgctggatgc
      541 gatggtcggc aaccccgaat attaccggat atgcggcgcg gtattcgtct gcggcacgcc
      601 gcttgccacc gatgtcggct ccgaatcgct ggtgaagggc aatggcatgg cggaagccag
      661 gaagctgctc gccgaatccg gctatgacgg cgcgccgatc gtgatcatgg cgcccggcga
      721 cgttgtgacc ctgaaagccc agccggtcgt ggcggcgcag ctgttgcgcg aggcggggtt
      781 caaggtcgac ctgcaggcca cagactggca gaccgtggtc gcgcgccgcg ccagccagaa
      841 gccgccgaag gagggcggct ggaatatgtt cttcaccaac tggtccggcg ccgacgtggg
      901 caatcccgtc gccaacgtgt cggtgggcgg caggggcacc aagggcggct ggttcggctg
      961 ggcggaggac gccaagatcg aggcgatgcg cgatgccttc gcgcgctcgt cctcccccga
     1021 ggagcagaag aagatcgccg ccgacatcca gaaggaaaac tacgaccagg tgatctatat
     1081 cccgcttggc caatatctcg cgccctccgc ctggcgaaaa tcgctgtcag gcgtgctcga
     1141 gggccctgcc accccgatct tctggaatat cgacaagtcg gagtagggcc gaggttgcct
     1201 ggctggcgcg aacgggggcg cggcccagcg tacggccccg gcctggagcg gggggatcgg
     1261 cgatggcgac tgcggcgcaa ttgcccacgc ggagcaacgc tcgtcatgcc cagcctcgtg
     1321 cccgacctgt ctttgagact caagcggtca gcagtgggtg ctgcggagcg actccaggcc
     1381 atcgatcagc gtcggcgggg catcctgcgg ccgcttgagc ggcgaatccg atcccttgcg
     1441 tagctggtgc tcggatgcct gacgctgatc aggatgagtt tggctggtca tgtagtgctg
     1501 ctccttttgc tgcagtacaa caatttcagt cgggtccagt tccgcgcgcg tggcgtcgat
     1561 cagtcagcac gtcgtgagcg gtccacgccg gcagctcacg gcgagttgaa gcaagcgggc
     1621 tttcacagct ccgggcgagg cgaagagcgt cgcccatcgc gatgcaaccg ccgcgctcgc
     1681 ggacacgggt ccggttgtgc aacggtaagg gcgccggcac ggctcggaag gctgggcgtg
     1741 ctggcgccca aagccaacaa cagtcagcca agcgcggtag ggtgttgacg aagcggatcg
     1801 aagtcgattg tcatgtagca acgagtacag aacgtgcttt cgcaatcgca catttgttcg
     1861 cagacaccta gcggcgcgac gcttgcgtcg gggggcgcaa cgtttcgagt ctgggcgccg
     1921 ggggcgaccg cggtctatct gcacggcaat tttggcggac aggtcttcga tcagctcacc
     1981 cccgaccgtt tgctgcaaaa aaatgcacgc ggctactgga ccggatttca ggacggcgca
     2041 cgcgatggcg accgctatcg cttctgggtt cagggcgcgg gctcgagcgg ctacaagcgc
     2101 gatccatacg cgcgcgagct cgagcctgag ggtttcccta actgcttcag catcgtccgc
     2161 gccagcagct ttccctggca cgatcaggat ttcgtgacgc cagacttttc cgacatgatc
     2221 atctaccagc tccatatcgg caccttcgca atccgcaagc cgggcgtgcc gtccaacctg
     2281 ctggacgtcg ccctgaaggt tccctatctc gccgagctcg gcatcaacgt gttgcagccg
     2341 cttccggtcg acgagcagga atcaaatccc agcatgggct atagcggcgc ggacctgttc
     2401 tcgccggatt tcccctacat cgccgtcgag gatctgccgt tctacctgga aaagctcaac
     2461 cagttctacg cggcgaagca aaaggccgcg atcacgctcg ccgacatcca gtccgcgccc
     2521 gggcaactca aggcgctggt cgacctctgc catgtcaacg gcatcgcggt cgtgttcgac
     2581 gtggtctaca atcacgccgg cggcttttcc gtcaacgggc agttcgacga ccattgcctg
     2641 tactacatgg atcgccgccc caatcgcggc aacaacaacg acagcctcta cttcac
//
LOCUS       DASWQN010000237         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_157071, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000237 DASWQN010000000
VERSION     DASWQN010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157071"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VGC82_03890"
     CDS             complement(<1..287)
                     /locus_tag="VGC82_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0422446.1"
                     /translation="MKGFHDYCRRSLDAIRDQGRYRIFMPLQKRAAGFPYYLRPDGSR
                     VLVWSSNDYLAMGGHPVPVNAACEAAQQMGAGAGGTRNISGTSPVHDELEA"
     gene            589..1221
                     /locus_tag="VGC82_03895"
     CDS             589..1221
                     /locus_tag="VGC82_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEY0422447.1"
                     /translation="MPLSQPAARERQHTRTIEINGYRRIDGDYDIEAHLTDTKTFGQT
                     NYDRGYIEAGEPIHDMWLRLTVDETMHIRAVEAVSDKTPYVMCPTAAPNFVRLVGMQV
                     KAGFLREASHAVGGTAGCTHLRELLQQMATTAFQTINPARVRREMAAEGVTDQKPGSD
                     GFDKRITEKWGNGNKILNTCLAYDANGPLVRRRWPHLYTGPEAASEAAAE"
     gene            1348..2916
                     /locus_tag="VGC82_03900"
     CDS             1348..2916
                     /locus_tag="VGC82_03900"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY0422448.1"
                     /translation="MKSYRLSRRLLFKTGTAASLCMPSISRAADRPAITHGVQSGDVT
                     MDAAITWARVNQPSRVRVEFATTDSFKTILGGGFTDALPESDLTMKFGLTSLPPGQRI
                     FYRVVPQNLSDPSIMGEAAIGHFRTAPTDKRSVSFVWSGDTAGQGWGINADWGGMKSY
                     ATMLKHDPDFFIHSGDTIYADGVIPAEITLKDGTIWKNLVTEAKSKVAETLADYRGNF
                     QYNLLDKNVLAFNAQVPMYVQWDDHEVTNNWWPEEPLTRAEHLRKKYTEKNMLPLVAR
                     SHRAFHEYMPIRTDPYEPARVYRKIGYGPLVDVFMLDERSYRGPNGEDRQTDYGPDAY
                     FMGPVQLAWLKSELKASKATWKVIAADMPISIFVVYDVDLKKGSEAISQLDNGKPLGR
                     ELEIADLLSFIRNEKIRNTVWLTADVHYTAAHYYDPNKAAFQDFEPFWEFVSGPIHAG
                     TFGPGELDQTFGPQLKFIKAPTAEQGQNAPPSAGLQFFGHVKIDGGTGQMTVTLRDVD
                     DNALWATTIDPVSS"
BASE COUNT          636 a          903 c          924 g          590 t
ORIGIN      
        1 tccgcctcca gctcgtcatg caccgggctg gtgccgctga tgttgcgtgt gccgccggcg
       61 ccggcgccca tttgttgcgc ggcctcgcat gccgcgttga cggggacggg atggccgccc
      121 atcgcgagat agtcgttcga cgaccagacc agcacccgcg agccgtcggg gcgcaaataa
      181 tacgggaacc cggcggcgcg cttctgcagc ggcatgaaaa tccggtagcg tccctggtcg
      241 cggatcgcgt cgagcgagcg ccggcaatag tcgtgaaaac ccttcatctc tttcgacgcc
      301 ttcggttcgc cagtccattg aaacctggtc agcttcatac ggaacgatac agtatattat
      361 cgatcggtag caaggaaatc gtcccgccgt ccccggttgc aatgagaaac atgcgctgat
      421 cggccgcttc gtcaactgcc gatcgcttgg ataaaccgga cgtgcctggc tgccagcggt
      481 tcgatccggt cgagggggca ggcatgcggc gggcgtgctt tgcaatcacg ctggcagtgc
      541 catatgttgc agcgcaatat tcgtctgcca tcgcttcagg agtccggaat gccgttgagc
      601 cagcccgccg cgcgcgagcg tcagcacacg cgtaccatcg agatcaacgg ctatcgccgg
      661 atcgacggcg actacgatat cgaagcgcac ctgaccgaca ccaagacctt tggtcagaca
      721 aattacgacc gcggctatat cgaggccggc gaaccgatcc acgatatgtg gctgcggctt
      781 acggtcgatg agacgatgca catccgcgcc gtcgaagccg tcagtgacaa gactccttat
      841 gtgatgtgcc cgaccgccgc gccgaatttc gtccggttgg tcggcatgca ggtcaaggcg
      901 ggcttcctgc gggaggcaag ccatgccgtc ggtggcactg ccggatgcac ccatctgcgc
      961 gaattgctcc aacaaatggc caccactgcg ttccagacca tcaatcctgc cagggttcgg
     1021 cgggagatgg cagccgaggg cgtgaccgat caaaaacccg gcagcgacgg ctttgacaag
     1081 cggattaccg agaagtgggg caacgggaac aagatattga acacctgttt ggcctatgac
     1141 gcgaacggcc cgctggtgag gcgccgctgg ccgcacctct acacaggacc cgaggcggcg
     1201 tcggaagcgg ccgccgaata gatgcttgga tggcggcggc gtggcctgtt gagaaggatc
     1261 gcttgatttc atccggtcgt cgccgaacag tcattatccg gtcatctctt gggcaggact
     1321 atctgtcctc acaaggggat catgattatg aagtcatacc gcctgtcgcg ccgccttttg
     1381 ttcaagacgg gaacagccgc ctccttgtgc atgccgtcga tcagccgagc cgccgaccgg
     1441 ccggcgatca cgcatggtgt gcaatccggt gatgtcacca tggacgccgc aattacttgg
     1501 gcacgtgtca accagccgtc acgggtgcgg gtcgaattcg ccacaactga ttcgttcaag
     1561 accattcttg gtggcggctt cacggacgcg ctgcccgaga gcgatcttac gatgaagttc
     1621 ggcctgacca gcctgccgcc cggacagcgg attttctacc gtgtggtgcc gcagaacctg
     1681 tccgacccgt ctatcatggg cgaggccgct attggccact tccgcactgc cccgaccgac
     1741 aaacgttcgg tgtcttttgt ctggtccggt gacaccgcgg gccagggctg gggcatcaac
     1801 gccgactggg gcggcatgaa gtcctatgcc acgatgctga aacacgatcc ggacttcttc
     1861 atccactcgg gcgacacgat ctatgccgat ggcgtcattc ccgccgaaat aactttgaag
     1921 gacggcacga tctggaagaa cctcgttacc gaggcaaagt cgaaggtcgc cgaaacgctt
     1981 gccgactatc gtggcaattt ccagtacaat ctgctggaca agaacgttct cgcgttcaat
     2041 gcccaggtgc cgatgtatgt ccagtgggac gatcacgagg tgaccaacaa ctggtggccg
     2101 gaggagccgc tgacccgcgc ggaacatctg cgaaagaaat acactgagaa gaacatgttg
     2161 ccgctggtgg ctcggtctca tcgggcgttt cacgaataca tgccgatccg gactgaccct
     2221 tatgaaccgg cccgggttta ccgcaagatc ggctacggcc cgctggtcga tgtgttcatg
     2281 ctggacgagc gcagctatcg cgggccgaat ggtgaggatc ggcagaccga ttacggtccc
     2341 gatgcgtatt tcatggggcc ggtgcagctt gcatggctga aaagcgagct gaaggcgtcg
     2401 aaggcgacat ggaaagtcat cgccgccgac atgccgatca gcattttcgt tgtctatgac
     2461 gtggatctga aaaaaggcag cgaggcaatt tcccagctgg acaacggcaa gccgttgggc
     2521 cgggaactgg aaatcgccga tcttctgtct ttcatcagga acgagaagat ccgtaacacc
     2581 gtctggttga ccgcggacgt gcactatacg gcagcacact actacgaccc gaacaaagcc
     2641 gcattccagg atttcgagcc attctgggaa ttcgtttccg gcccgatcca tgccggcacg
     2701 tttggtccgg gcgaactgga tcaaacgttc ggcccgcaac tgaagttcat caaggcgcca
     2761 acggccgagc agggacagaa tgcgccgcct tcggccggtc tgcaattttt tgggcatgtc
     2821 aaaatcgatg gcggtactgg gcagatgacc gtgaccctgc gcgacgtgga cgataatgcg
     2881 ctttgggcga cgacgattga tcccgtcagt agctgaccgg cgcgatgccc cgatccggtt
     2941 aggcaattct gtgccgcgct gctaccgatc aagccttcga caccgatcga agctaccgat
     3001 aggcagaata ttcggaggaa cgtcatggcc gacatagcaa aagtcgccga ggg
//
LOCUS       DASWQN010000238         6420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_157397, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000238 DASWQN010000000
VERSION     DASWQN010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157397"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VGC82_03905"
     CDS             <1..1302
                     /locus_tag="VGC82_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0422449.1"
                     /translation="DRPRCVYVDFLSTGPAVSKTLRGVVQRTPCIERLGDALVTDLVT
                     RDGVVTGAVVIHIETGEVLTISAKATIIATGGLTRLYRRNSASANMGGDGYALALRAG
                     AELIDMEFVQFFPIGHLAPRLIGMDPIMWDPFRYKLGGRLLNGLGQEFVDRYGSSDDG
                     RYVVTRDLATYAITKEVEAGRGSPSGGAWLSFRHIPPAVMKQAFGPVIDRLHAAGIDL
                     GRDDVEVAPIAHYHMGGIRVDRRMTTGVSGLYAAGEAVGGASGANRLSGNAITEALAF
                     GRTAGRSAAEQGGGADFHPEAAAASVMLAGAEGPPCATAALFVRLQSVMSDHVGPFRT
                     RQGLAAALDAVHELRAEAGSVPPGKPQAHDLGRVDWFDLRQGLLVAEAVILAASAREE
                     SRGAHQREDFPEINEAWTRNQVLRLTHRRLQLTACPVPEAT"
     gene            1299..1625
                     /locus_tag="VGC82_03910"
     CDS             1299..1625
                     /locus_tag="VGC82_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0422450.1"
                     /translation="MSQTATLLIDRGGRHERFDVRFEQGQSVLDGLRQIRATTDPSLA
                     FRYSCLNANACKECMMLLDGKVIYACTARLEAREMRLAPLPNKARLRDLATVIAPPDE
                     RLRAVR"
     gene            1738..1953
                     /locus_tag="VGC82_03915"
     CDS             1738..1953
                     /locus_tag="VGC82_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422451.1"
                     /translation="MRKIILAVILVGFSVSAYAADEKKPAGGVKGAAVGAAAGHEVGS
                     GHAAAGAATGAAIGHHQTKKAEESKSQ"
     gene            2020..3033
                     /locus_tag="VGC82_03920"
     CDS             2020..3033
                     /locus_tag="VGC82_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEY0422452.1"
                     /translation="MTSATVLYSENRYPDDSVERRIYGPDVRVIFPSASASMSDLSDE
                     DCAAAEGLMILSFRLSADDLKRFPRLRAICRMGVGYDNLDRVAAAERQIMVLNVPDYG
                     TTEVADHAMALALSLRRGLLLHHETQRAHPPAAWRYIDDPLVRRSSVQTFGIVGMGRI
                     ATAVALRAKAFGFRVLFYDPHLPNGVELGLGIQRAATLDELLSQSDTLSVHAPLTRDT
                     RGLLGLRELSLLPKGAVVVNTARGPIIDIDALATLLKRGHLAGVGLDVVPVEPPVEPV
                     PELMRAYRAREDWTLGRLIITPHSAFYTPQAWDDIRTKSAETMRAALLGPRPQNVVPP
                     DSD"
     gene            complement(3092..3442)
                     /locus_tag="VGC82_03925"
     CDS             complement(3092..3442)
                     /locus_tag="VGC82_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422453.1"
                     /translation="MTRLWLTANAFAAAMLCALPGSSRELLAGSSRVRKVPGQGVMIP
                     SDGDIVRMDPGTDADCAIGNASRLLIEAAGPGVHGNLFCGDARIRTAFVRNSTRTVPE
                     LFDNTLSGNVQTLE"
     gene            3541..4593
                     /locus_tag="VGC82_03930"
     CDS             3541..4593
                     /locus_tag="VGC82_03930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0422454.1"
                     /translation="MVHVFPTFAVGGAQVRFAALANHFGRAFRHAVVSLDGDLACRER
                     LCADLDVTFPVVAAAKDAMLSNAWRYRRLLRNWQPDGLVTYNWGAIEFALANIVPVSR
                     HVHVVDGFGPEERSVQIPRRVLMRRIALLRSPVVLASRNLLRIATDVWKLPPKALYYI
                     PNGIDLAQFASDGTRRAGTEIVIGTVAALRQEKNIARLLRAFATLPSGRLVIVGDGPE
                     RSALEALATTLGIAPRVCFAGHHRETAPFYAEFDIFALSSDTEQMPLSVIEAMASGLP
                     VVSTSVGDVRLMVADENARFITALDDSALAGALAALAGDRSVREKIGLANLAKAWRDF
                     DQAAMFAAHGALWHGV"
     gene            4590..5693
                     /locus_tag="VGC82_03935"
     CDS             4590..5693
                     /locus_tag="VGC82_03935"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0422455.1"
                     /translation="MTVVRVGGVEDFTALGARWRDLEQRADGSFFQSWTWVGCLCAER
                     FPDPVLVEASEAGHIVALGLFNRVRHRIGPSTLYLNETGTAAFDCPYVEQNGILTEAG
                     REQELTALCLRQLVRTHDVVLSGVGEQVLAAFRSAAGVVLTGPGKASPFVDLAAVRKT
                     GNDYLAGRSANTRQQLRRSDRFYERDGPIWLEPAGSVEAAHAMLEQMAELHQTTWRAR
                     GKQGSFALPFFRRFHRELIASGVPRREVALLRISCGKTVIGVLYNFVWHKRMSAYQSG
                     FAYHDDESHAKPGLTCHHAAIRLGLQQGMDIYDFLAGGDRYKRSLAEGAYQQFWARAG
                     PYWSVRQWLLRCRDLTRRQPSGALFEPVQGTQK"
     gene            5777..>6420
                     /locus_tag="VGC82_03940"
     CDS             5777..>6420
                     /locus_tag="VGC82_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422456.1"
                     /translation="MLALLLVEVQETRAAPTGGGVSSPSLDFGNVLVGDAAVSLSDTA
                     TINGGAGTTLSFGTPSSPFGGGGQTVAVTNNTSQSLIGTFTFAPLTTGAATPQNVLVS
                     DVGAGGTFTSPIALSGSGVAPSQAITSSNPYVLIGQSGPVSLTVSNTGNGNLSGRGTV
                     SNLRGTVSAANSVFVGTGGSFSLGDSTQTTTPTTTTFAYTFAPTKVGAASTVVST"
BASE COUNT         1092 a         1943 c         2189 g         1196 t
ORIGIN      
        1 gatcgtcccc gctgcgtgta cgtggatttc ctgtccaccg gcccggcggt gagcaagacg
       61 ttgcgcggcg tcgtgcaacg gacgccttgc atcgaacggc ttggggatgc gttggtgacc
      121 gacctggtga cccgggacgg cgtggtgacc ggtgccgtcg tgattcacat cgaaaccggc
      181 gaggtgctga ccatcagtgc aaaggcaacg atcatcgcga ccggcggcct gacaaggctg
      241 tatcgccgca acagcgcgag cgccaacatg ggcggcgatg gctacgccct ggcgctgcgg
      301 gccggggcgg aactgatcga catggagttc gtgcagtttt tcccgatcgg gcacctcgcg
      361 ccgaggctga tcggcatgga cccgatcatg tgggacccgt ttcgctacaa actgggggga
      421 cggttgttga acggcctggg ccaggagttt gtcgatcgct acggcagctc cgatgacggc
      481 cgctacgtgg tgacgcgcga cctcgcgacc tatgccatta ccaaggaggt cgaggcgggc
      541 cgaggttcgc ccagcggcgg ggcctggctt agttttcgcc acatcccacc ggcagtcatg
      601 aagcaggcgt tcggaccggt catcgaccgg ctgcacgcgg ctggcatcga tctgggacgt
      661 gacgatgtcg aggtcgcgcc gatcgcccat taccacatgg gcggtatccg cgtggacagg
      721 cggatgacga ctggcgtgtc cgggctttac gccgccgggg aggccgtggg cggggcgagc
      781 ggcgccaacc gcctgtccgg caacgcgatc accgaggctt tggcgttcgg ccggacggct
      841 gggcgaagcg cggccgaaca gggcggcgga gccgacttcc atccagaggc cgccgccgct
      901 tccgtcatgc tggccggtgc cgaaggtccg ccctgcgcta ccgccgcgct gttcgtccgc
      961 ttgcagtccg ttatgtccga tcatgtcggg ccattccgaa ccaggcaggg cctggccgca
     1021 gcgctcgatg cggtgcatga actgcgggcc gaggcgggtt cggtgccgcc cggcaagccc
     1081 caagcgcacg atctcggccg ggtggactgg ttcgatttgc gtcagggctt gctggtggcc
     1141 gaagcggtca tccttgcggc atccgctcgg gaagaaagcc gcggcgctca ccagcgggag
     1201 gattttccgg agataaacga agcctggacg cgcaatcagg tgctgcggct cacccaccga
     1261 cgccttcaac tgacggcctg tccggttccc gaggctacat gagccagacc gcgactctgc
     1321 tgatcgaccg gggtgggcgc catgagcgct tcgacgttcg cttcgaacaa ggacagtccg
     1381 tgctggatgg gctgcggcag atccgagcca cgaccgatcc gtcgctggcg ttccgctatt
     1441 cgtgcctcaa cgccaatgcc tgcaaggagt gcatgatgct gttggacggc aaggtgatct
     1501 acgcctgcac tgcccgtctg gaagcccgcg aaatgcggct cgctcccttg ccgaataaag
     1561 ccaggctgcg cgatctggcg accgtgatcg ctccgcccga cgagcggttg cgcgcggtac
     1621 ggtaacggcc ggcgtgccgg caggcaaata tgcaacagca ccgggatatt ggtctcggtg
     1681 aagcgaaaca ttttctgccg gagcgcgtta aacctcccac tgaaacggga ggttgttatg
     1741 cggaagatca ttttggcggt tatcctggtt ggcttttcgg tctcggctta cgccgcggac
     1801 gagaagaagc cggcgggcgg ggtaaagggt gccgcggtgg gcgctgccgc ggggcatgag
     1861 gtcggcagcg gacacgctgc tgccggtgcg gcgaccggag cagcgatcgg acatcaccag
     1921 accaagaagg ccgaagagtc gaagtcgcag tagcagcgga tttgcgtcag gtagggtcgc
     1981 gcgataggag ggcttgaaga cctcaaggaa acgcccgtca tgacctccgc caccgtgctg
     2041 tattcggaaa accgttatcc cgacgacagc gtggagcggc ggatctatgg cccggacgtg
     2101 cgggtcatct tcccctcggc atcggcctcg atgtccgact tgtcggacga ggattgcgcc
     2161 gcggccgagg ggttgatgat tctgagtttc aggctgtccg ccgacgattt gaagcgcttc
     2221 cctcggctgc gggcgatctg ccggatgggg gtcggctacg acaacctcga ccgggtcgcc
     2281 gcggccgaac gccagatcat ggtcctgaac gtgcccgact atggcacgac cgaggttgcc
     2341 gatcatgcca tggcgctggc gttgtcgctg cggcgaggtc tgctgttgca tcacgagacg
     2401 cagcgggcgc acccgccggc cgcttggcga tatatcgacg acccgctggt tcggcggtcg
     2461 tcggtacaaa cgttcggcat tgtcggcatg ggacggatcg ccacggcggt ggcgttgcgg
     2521 gcaaaagcct tcgggttccg agtgttattt tatgatccgc acctgccaaa cggggtcgaa
     2581 ctcgggctag gcatccagcg ggccgcaaca ctcgatgagt tgttgagcca gagcgacacg
     2641 ctgtccgtcc acgcgccgct gacccgtgat acgcgcggac tgcttggcct gagggagctg
     2701 tcgctgttgc cgaagggcgc ggtggtggtg aacaccgcac ggggtccgat catcgatatc
     2761 gatgcgctgg cgacgctgct gaaacgcggt cacctggccg gcgtcggcct ggatgtggtg
     2821 ccggtggagc cgccggtcga gccggtcccg gagctgatgc gggcctatcg ggcgcgggag
     2881 gattggacgc tggggcgttt gatcatcacg ccgcactcgg cattttacac gccacaggca
     2941 tgggacgata tccgcaccaa gtcagccgag acgatgcgag cggcgttgct tggcccccgg
     3001 ccgcagaacg ttgttccccc agacagcgat tgatcgccgt ctcgccagcc tccggacgcg
     3061 atcaccgcga agaggcgatg tttcttccgt atcattccag agtctgaacg ttaccgctta
     3121 gcgtgttatc gaacagttcg ggaaccgtcc gggtgctgtt gcggacgaac gccgtccgaa
     3181 tccgcgcgtc gccgcagaaa aggttgccgt ggacacccgg ccccgctgcc tcgatcagta
     3241 gtcgcgacgc attcccgatt gcgcagtccg catcggtgcc gggatccatg cgcacgatat
     3301 cgccatcgga cgggatcatg acaccctgac ccggcacctt ccgtacccgg gatgagccgg
     3361 ccagtagctc ccgcgacgaa ccgggcaggg cacaaagcat tgcggcggcg aaagcgtttg
     3421 cggtaagcca cagccgggtc atgcccgtgc tgtcggcgca aaacttcccg tgctgtcagc
     3481 gcaaaacttg aggacttcac ggtgaaactg gctaccggaa agacagtgtc ggctccattg
     3541 atggttcatg tttttccgac cttcgcggtg ggaggcgccc aggtgcgctt cgcggctctc
     3601 gccaaccatt tcggccgagc gttccgccat gccgtggtgt cgctggacgg cgacctggcc
     3661 tgccgcgagc ggctctgcgc ggatctcgat gtgacgttcc ccgtcgtcgc cgcggccaag
     3721 gacgcgatgc tgtcgaacgc gtggcgatac cgacgcttgc tgcggaactg gcagccggat
     3781 ggactggtca cctacaactg gggcgcgatc gagttcgccc tggcgaacat cgtgccggtc
     3841 agccgccatg ttcacgtcgt cgatggattc ggaccggagg aacgttccgt ccagatcccg
     3901 cgccgggtgc tgatgcggcg gatcgcgctg ctccgctcgc cggtggtgct ggcctcgcgc
     3961 aacctgctgc ggatcgcgac ggacgtctgg aagctgccgc cgaaagcgct ttattatata
     4021 ccgaacggta tcgatctcgc gcagtttgca agcgacggga cgcggcgtgc ggggaccgag
     4081 atcgtcatcg gaaccgtcgc ggcattgcgg caggagaaga acatcgcgcg gctactgcgg
     4141 gcattcgcta cgctgccgtc cggccgcctg gtgatcgtcg gcgacgggcc ggagcgatcg
     4201 gcgctcgagg ctctggccac cacgctgggc atcgcccccc gggtgtgctt tgccggacat
     4261 catcgggaaa cggcgccgtt ctatgcggag ttcgatattt tcgccttgtc gtcggatacc
     4321 gaacagatgc cgctctcggt gatcgaggca atggcgagcg gactgccggt cgtgtccacc
     4381 agcgtcggcg atgtgcggct gatggtggcg gatgagaatg cgaggttcat cactgccctg
     4441 gacgattctg cgttggcggg cgccctggcg gcactggccg gcgatagatc ggttcgagag
     4501 aagatcggtc ttgcgaacct ggcgaaagct tggcgggatt tcgatcaggc cgcgatgttc
     4561 gccgcccatg gtgcgttgtg gcacggggtt tgacggttgt tcgggtcggg ggcgtggaag
     4621 actttaccgc cttgggcgcg cggtggcgcg atctggagca gcgtgcggat ggctccttct
     4681 ttcaaagctg gacctgggtc ggctgcctct gcgcggaacg ctttcccgat ccagtattgg
     4741 tcgaagccag cgaggccgga cacatcgtcg cactgggttt gttcaatcgc gtccgccacc
     4801 ggatagggcc gtccacgctg tatttgaatg agaccgggac agccgcgttc gattgcccct
     4861 acgtggagca gaacggcatc ctgacggaag cgggacgtga acaggaactg acggctttat
     4921 gtttacggca attggtccgg acgcatgacg tggtgctgtc cggggttggg gaacaggtct
     4981 tggcagcctt ccgctccgcg gctggcgtgg tgctgaccgg cccgggcaaa gcctcgccat
     5041 tcgtcgatct ggcggcggtc agaaaaaccg gcaacgatta tctcgctggg cgaagtgcca
     5101 atactcgtca gcaactgcgc cggtccgacc ggttctatga acgggacgga ccgatctggc
     5161 ttgaaccagc cggatcggtc gaggcagcgc acgcgatgct ggagcaaatg gcggaactgc
     5221 accagaccac atggagggcg cgcggcaagc agggcagctt cgcgttgccg ttcttccgcc
     5281 gtttccaccg cgaactgatt gcctccggcg tcccgcgacg ggaggtggcg ttgttgagga
     5341 tatcgtgtgg gaaaacggtg atcggcgtcc tgtataactt cgtctggcat aagcggatgt
     5401 ccgcctacca aagcggattc gcttaccatg acgacgagag ccacgccaag ccgggcctga
     5461 cctgccacca tgcggcgatc cgattggggc tgcagcaggg gatggacatt tatgacttcc
     5521 tggccggtgg cgatcgctat aagcgaagcc ttgccgaggg ggcgtatcag cagttctggg
     5581 cgcgggccgg cccatattgg tcggtgcgcc agtggctgct tcgctgccgg gatttaaccc
     5641 ggcgtcagcc aagcggcgct ctctttgaac ctgtccaggg gacgcaaaaa tagttgcgta
     5701 tcatcaaatg ttacgatata cgggcagaga agggtagggc tgtagcttga gggcgatact
     5761 ggcatccgcg acctcgttgc ttgcactcct cctggtggag gtgcaggaga cgcgtgccgc
     5821 accgacaggg ggaggcgtca gctcaccctc gctcgacttc ggcaacgttc tggtcggtga
     5881 tgccgcggtc agtctgtcag ataccgcaac gatcaatggt ggcgccggca ccaccctgtc
     5941 atttggcacg ccgagcagcc cgtttggcgg cggcgggcag acggtcgcgg tcaccaataa
     6001 tacttcgcaa agcctgattg ggaccttcac cttcgctccg ctgacaacag gcgcggcgac
     6061 gccgcaaaac gtcctggtgt cggatgttgg tgcgggcggg acgttcacaa gtccgatcgc
     6121 tttaagcggt tcgggcgttg cgccgtcgca ggcgatcact tcgtccaatc catacgtgct
     6181 gatcgggcaa tccgggccgg tcagcctaac cgtcagcaat accggaaacg gcaacctgtc
     6241 gggccggggc acggtcagca atctgcgtgg gactgtctca gccgccaatt cggtgttcgt
     6301 gggaaccggc ggctcgttct cgctcggcga ttcgacccag acgacaacgc ctacgaccac
     6361 gactttcgcc tatacgttcg cacctaccaa ggtcggggcg gcctccacgg tcgtctccac
//
LOCUS       DASWQN010000239         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_684914, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000239 DASWQN010000000
VERSION     DASWQN010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684914"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..79
                     /locus_tag="VGC82_03945"
     tRNA            3..79
                     /locus_tag="VGC82_03945"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Pro,seq:ggg)
     gene            943..2385
                     /locus_tag="VGC82_03950"
     CDS             943..2385
                     /locus_tag="VGC82_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0422457.1"
                     /translation="MSDDSAVQPASGRLAHQGGLRGKIFEGGALLMGRQLVSMGLSLI
                     GLLVITRIIGPAAYGPYVAALGICQYAQNLGQAGIGVYLVRAAGDVTPRTFDIATTLL
                     LISALGLMVVLEASLGLIATWIPMPGLAPLLGVLLISVVFQTIAVGASAQLERALDFR
                     RVAMIEMAGQLLYYAVTLPLVFMGFGVWSLVAGWCLQQVFVCVAFYVAARYWPKLAWD
                     TAIVRSMLNYTLGYAASDWLWQLRNLINPLIVGHFLPAEAVGQVGLAIRMMELLSFTK
                     TIAYRLSVAVLAKVQHEPAKLIAAATDGMRLQTLALGPVLLGFSWFGGFLLAMVFGKR
                     WEPVMLVYPYLALSYLTNAQFNIHSSILYVLHRNWAVSWFHIVHVVLFAGSAWVFVAR
                     FGIVGYGYAEMAALLSYPVVHRSVRQAVGSPDYAISALWWGGIAIGLFWRELGPWAIV
                     APVAVLLCPPSLRQVKVFYGMFARRPRHVI"
     gene            2375..3430
                     /locus_tag="VGC82_03955"
     CDS             2375..3430
                     /locus_tag="VGC82_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422458.1"
                     /translation="MSSENFRFFVLTCRDPKSDFRQPLVAALRRHYPTYYIWLRRRPV
                     VSGPGTNEGATEMSLLNFLRFMRRFRQDNCINIYFNSLNTYFPGLTAILRVIATAGVW
                     CLDMHDDLRYHNLGFKRWRESMIIAFLRRLSHVTVHAATTLQEQFPDAQHLGNASSIQ
                     PLCLPDLNSNDILIIASFDPRFDFDFLAELAEKCPTNRFHLHGWTHPKDPASMTRILA
                     ATARYSNVYYHGPYTMADLPAILSRYRISVAPYRADSMLTRYIDPLRFYHCLNAGLEV
                     ITTNIPQARQMADWLHVVSDATACADTLAAIQAGRLAKQPAYSPITWEQRADRLVEIL
                     RSLPRTIKLRRRRRAAQ"
BASE COUNT          618 a         1097 c         1064 g          735 t
ORIGIN      
        1 gtcggagcgt agcgcagcct ggtagcgcat cagtctgggg gactgggggt cgtgggttcg
       61 aatcccgccg ctccgaccaa cgttaccgtt tccccgcatc atcatgacgg ctacatgaca
      121 atttgttatg gtggtcgccc agtccgctca gcaagcaaca acttgcgtac ggagatcagg
      181 gagatcgtcg tggatggtcc gccgaacagt cccattgtcc agttcgcgat agcatggatc
      241 ggcgcatggc gttcgcgaac cgaagatcgg gcagttcgcg gcgagttccg gagcctcccg
      301 aggaagccgc ttgagtgcct caccggcgat ttcgaattgg cattccattg ccgaacgaag
      361 ccatcaggtc ggcgacgtag tcacaaacgc agcgatgcta tcggcggcct gacaaacgtc
      421 ccacagccag gcacgcgggt cacgttgcat agatcgcctg ccacgagctt ccgattcccg
      481 cccggatgaa ggggttctca accgcggttt ccatcaccag atcgaccggc ggccaaacag
      541 ctccgccaaa tcgtctcgga gatcctggta ggcggcgaca ccgggatggc ggccaggacg
      601 ataagtgacc atcaggtcaa catccgaacg gtccggatcg aagtcggaac cgcgcggcgg
      661 atccaaacac gtcgagccga cgaacgtgaa accgctggca gagttcggcg atgtctggtc
      721 ggcgggtctc gatcaggtac agcatgggcc gattatccgc ctgatcacgc cgcactttaa
      781 gcctatcctc accttcaggc gacccactga acaggctttg atcgggccgg cgcgaccgga
      841 gtggtattaa cccttgtcac ggcgacacga cagccgagat cagcctttgt gcaccgcagc
      901 gcggatcaag ccgaaccccc ttccctccca cccaggtgat cgatgagcga cgatagcgcg
      961 gtccaacccg cctccgggag actggctcac caaggcggcc tgagaggcaa gatcttcgag
     1021 ggcggcgcgt tgctgatggg gcggcagctc gtctcgatgg gtctgagcct gatcgggctg
     1081 ctggtcatca cccggatcat tggcccagcg gcgtacggac cctacgtcgc cgcactcggc
     1141 atctgccagt atgcgcaaaa cctcggccag gcggggatcg gcgtttacct ggttcgcgcc
     1201 gcgggcgatg tcacgccgcg cacgttcgac attgccacga cgttgctgct catttcggcc
     1261 cttggtctga tggtcgtcct ggaagccagc ctggggctga tcgccacctg gattccgatg
     1321 cccggcctgg caccattgct gggtgtgctg ctgatctcgg tggttttcca gaccatcgcg
     1381 gtcggggcga gcgcgcagct ggagcgcgca ctcgatttcc gccgcgtggc gatgatcgaa
     1441 atggcggggc aattgctgta ttacgccgtt acgctgccgc tggtgttcat gggcttcggc
     1501 gtttggtccc tggtcgccgg ctggtgcctg cagcaggtct tcgtgtgcgt cgcattttac
     1561 gtcgccgcac gctactggcc aaagttggcg tgggacaccg ctatcgtccg ctcgatgttg
     1621 aactacacgc tgggctacgc cgcgtccgac tggctgtggc aattgcgcaa cctgatcaac
     1681 ccgctgatcg tcggacattt cttgccggcc gaggccgtcg gccaggtcgg gctggcgatc
     1741 cggatgatgg agttgctgtc gtttactaaa acgatcgcat accgcctgtc ggttgccgtc
     1801 ctggccaaag tgcagcatga accggccaag ctgatcgcgg cggcgaccga cggcatgcgg
     1861 ctgcaaaccc tggcactcgg acctgtcctg ctcggattca gttggttcgg cggcttcctg
     1921 ctggcaatgg tcttcggcaa gcggtgggag ccggtgatgc tggtgtaccc ctatctggcg
     1981 ctgagttacc tgaccaacgc gcagttcaac atccattcgt ccattctgta tgtgctgcac
     2041 cgcaactggg cggtgtcctg gttccacatc gtccatgtcg tgctgttcgc cggatcggcc
     2101 tgggtgtttg tcgcccgctt cgggatcgtc ggctatggct acgccgagat ggcggcgctg
     2161 ctgagctacc ccgtcgtcca ccgctcggtg cgccaagccg ttggctcacc ggactacgcg
     2221 atctcggcat tatggtgggg aggcatcgcg atcggattgt tctggcgtga actggggcca
     2281 tgggcgatcg tggcgccggt cgcggtgctg ctgtgtccgc catcactgcg gcaagtgaag
     2341 gtcttttatg gcatgttcgc ccggaggccc cggcatgtca tctgagaact ttcgcttctt
     2401 cgtactgacc tgccgtgacc cgaaatccga ttttcgccag ccgctggtcg cggcgctgcg
     2461 gcgtcattac ccgacctatt atatctggct tcgccgacgc cccgtggtgt ccggccccgg
     2521 cacgaacgag ggggcgaccg agatgtcgtt gctgaacttc ctgcgcttca tgcggcgctt
     2581 tcggcaggac aactgtatca acatctattt caacagcttg aacacctatt ttccaggtct
     2641 gaccgccata ttgcgtgtga tcgcgacggc cggcgtttgg tgcctcgata tgcacgatga
     2701 cctgcgatac cacaacctgg ggtttaaacg ttggcgggaa agtatgatca tcgctttcct
     2761 gcgccgcctg tcgcatgtca cggtgcatgc cgcaacgacg ctacaagaac agtttccgga
     2821 cgctcagcat ctcggcaacg cgtccagcat tcagccgctt tgcctaccag acttgaacag
     2881 taacgacata ttgatcatcg ccagctttga tccaaggttc gactttgact tcctggcgga
     2941 gttggccgaa aaatgcccca caaaccggtt tcatctgcat ggctggaccc atccgaagga
     3001 cccggcgtca atgacgcgga tcctggcggc cacagcacgc tacagtaatg tttactacca
     3061 tggaccctat accatggccg atttgcctgc gatcctcagc cggtaccgga tcagcgtggc
     3121 accatatcgc gcggactcga tgctgacccg gtacatcgat cctttgcgtt tctaccattg
     3181 tctgaacgcc ggcctcgagg tcattaccac caacattcca caggcccgac aaatggccga
     3241 ctggctgcat gtggtttccg acgctacggc ttgcgcggac acgctggcag cgatccaggc
     3301 tggccggctg gccaagcagc cggcttattc gcccattaca tgggaacagc gggcggatcg
     3361 cctggtggaa atcctgcggt cgctgccgcg caccatcaag ctgcgccgcc gcaggcgcgc
     3421 cgctcaatga tcgggaagtg cggcctgtcg cagcctgcca tagaccgcct cctgttcggg
     3481 tctcggcgtt ttccaatcgg cttccaacga gccg
//
LOCUS       DASWQN010000240         2658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_817661, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000240 DASWQN010000000
VERSION     DASWQN010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817661"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            510..1679
                     /locus_tag="VGC82_03960"
     CDS             510..1679
                     /locus_tag="VGC82_03960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0422459.1"
                     /translation="MQLRSWIILAALALARVAFGYQFQTVATLGTELVQRFGLSYAQL
                     GSLIGAYMLLGVFMALPLGLLGRRFGDRLVLGAGLALMTAGSCISAWADGAAGIAAGR
                     SVAGVGAVAMIVLQGKIIADWFVGVRFMIGISVSVCAFPIGMGLAQLVLPPVLMRSGM
                     HAAFLTDAVPAGMALLLFTLSYREPAHAASVPRRFSLPSGRECLLLAVAGSIWTVYTS
                     GFSSFASYLPSSLSGRGYSLTVTTLVVTVATWGNVPGTLAGGGLAARFGGFNIFLIGS
                     LSLAVGMAGSALTGWPVAWAVLLGVVGSIQPGVIMAVGTLSARPENRAVGMGLFYTLY
                     YLGGTFAPALCGIVADYSGQPEGGLLAAAAISALAIPFYMLHRALASHETMLVRA"
     gene            complement(1874..2251)
                     /locus_tag="VGC82_03965"
     CDS             complement(1874..2251)
                     /locus_tag="VGC82_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HEY0422460.1"
                     /translation="MAATAIQATTTVAFRTAGIKPSSVHIFAKSGRKWIVASEVCRIL
                     GIRTDNVPKLVPEQDRGRAAVLTKGSVQSVTTITEDGFNLLVAQSTKPVAKALHEWMV
                     KEALPAIPKALAPKVPVGQGDLF"
BASE COUNT          432 a          775 c          874 g          577 t
ORIGIN      
        1 cgccagacca tctcgcccgc tccgcaggtc gacattcggc gtgtcccggc gcggtcgatc
       61 tcggcgatgg cggcagcggc gttggacagt agcatgcacc cgaccttggt gcggcacctc
      121 cgccgcgtca atcatctgcg cgcattccat gcaaaggtcg ggaaccagtg tccgcgcagc
      181 tctgttgcgg taacctcact gttgggaagg tcgggtttgg gcaggcatct ggtgattacg
      241 ctggaggtgt aaaatcgtgg gcgatgccgc cagtcacgta acaggtcgcc gccggcacag
      301 cggcacgtcc gcggctgatg ccacccgcac cgaaagtgtc attgcgttcc accaagctga
      361 atcgcgtcgg ttagtcgtaa atcacccaag cggtcatagc acacccagag gttggagcca
      421 gagttgccat atcatggggt attcacctct tgcctgactg gatagggtcc gcgcggtccg
      481 gcgcggaacc ggcactcctg aggaaacgga tgcagcttcg ctcctggatc attctcgcgg
      541 ccctggcgct cgctcgggtt gcgttcggct accagttcca gacagtcgcc actttgggca
      601 ccgagttggt tcagcggttt ggcctgagtt acgcccagtt aggcagcctg atcggcgcct
      661 atatgctgct gggtgtgttt atggctttgc cgcttggctt gctggggcgt cgcttcgggg
      721 atcggttggt gctgggcgct ggcctggcat tgatgaccgc gggctcgtgc atcagtgcct
      781 gggcggatgg ggcagcggga atcgcggcgg ggcgcagcgt ggcgggcgtt ggcgcggtgg
      841 cgatgatcgt gctgcagggc aagatcatcg ccgattggtt cgtcggcgtg cggttcatga
      901 tcggtatcag cgtgtccgtc tgtgctttcc cgatcgggat ggggctagcg cagcttgttc
      961 tgccgccggt gctaatgcgc tccgggatgc atgcggcctt cctgaccgac gctgtaccgg
     1021 ccgggatggc gttgttgctg ttcaccctca gctaccgcga gccggcgcat gccgcctcgg
     1081 tgcctcgccg cttctcgctg ccgagcgggc gcgaatgcct gttgctggca gtcgcgggga
     1141 gcatttggac agtctatacc tcgggctttt catcatttgc atcatatttg ccgagcagcc
     1201 tgtcgggtcg tggctacagc ctgacggtca ccacgctggt tgtgacggtt gccacctggg
     1261 gcaacgtgcc cggcactctg gccggtggcg gactggccgc ccggttcggc ggtttcaaca
     1321 tcttcttgat tggttctttg tcgttggctg tcggcatggc aggttcggcg ctgaccggct
     1381 ggcccgtggc gtgggcggtt ttgctcggcg tggtaggttc gatccagccg ggtgtaatca
     1441 tggcggtcgg gactctgtct gcccggccag aaaaccgcgc ggttggcatg ggtctcttct
     1501 acacgctgta ttatctgggg ggcacgtttg cacccgctct gtgtggcatt gtggcggatt
     1561 attccggcca accggaaggt ggactgcttg ccgccgcagc tatttcagcg ctcgccatcc
     1621 cattttatat gcttcaccgg gcgctggctt cgcatgagac gatgctggtt cgggcttaga
     1681 attcgccggg agcccaactg cagtggctta cggctgtagg agaggttccg gccgatgtgc
     1741 cgcagggaac gaaaaggggg cgaaggcggt gctggacgtt gcaaagatcc tggccgctgt
     1801 tgagcataaa gggcaggcac aacttaaccg gatgaagcac aatccaacgc tgggtcgccg
     1861 catgtaagct gcctcagaag agatccccct gcccaaccgg aaccttcgga gccaaggcct
     1921 ttgggattgc gggcagcgcc tcttttacca tccactcgtg cagtgccttc gcaacgggtt
     1981 tggtcgactg tgcgaccaaa aggttgaagc catcctcggt gatcgtggtc acggactgca
     2041 cactgccttt ggtgagcacg gcggcacggc ctcgatcctg ctctggaacc agcttcggca
     2101 cgttgtccgt gcgaatgccg aggatgcggc agacctcgct cgcgacgatc catttgcgtc
     2161 cgctcttggc gaatatgtgg acagacgagg gcttgatgcc cgcggtcctg aaggcgacgg
     2221 tggtggtggc ctggatcgct gtagcagcca tgatggtgta acctcctggc aaaacaatca
     2281 tccttgcggc agctggattc gggcaccgcc atccgcctgg ttctcctgac caacatcgtc
     2341 gccaagatga aggtctgcgc tcttaacggt tacgcgagcc ggaatgcccc acgatgatct
     2401 tgtcgaacag ccgagacctt ggccaacaag atagagccgg tcatctggcc gaacgcgggc
     2461 ggcgacaccg acgcactggt atgcgctgac cttgatggcg ttcctcggca tcaagaccat
     2521 gtcgaacgcc cgaggcgcat tcagcaacgg aaccaaccgc atcctgcagg ggctacgcat
     2581 gaaatgtcgt gtcggagttg catcgggctc attggctatc ccgccaggag cttgagcatg
     2641 taggcgtcat tccaggcg
//
LOCUS       DASWQN010000241         3952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_25944, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000241 DASWQN010000000
VERSION     DASWQN010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25944"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..580)
                     /locus_tag="VGC82_03970"
     CDS             complement(173..580)
                     /locus_tag="VGC82_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422461.1"
                     /translation="MDGIGAFCGAILLALSLRPTWYARAYLGGVTAYMVMLAVFALAP
                     NPAIAGGALLLTGLGGAGFSTMQATLVFLASPPEMRSRLLGVLSMCIGAGPIGFIALG
                     MLADAIGAHWATAATGAAGLVALALTRPLWRHI"
     gene            complement(626..1441)
                     /locus_tag="VGC82_03975"
     CDS             complement(626..1441)
                     /locus_tag="VGC82_03975"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0422462.1"
                     /translation="MKHFLKTRAGVTAVARATASPLGRKRPLQQLLIAGCPDFWRLWS
                     VGLVVFTVRWLETVAIGVFVYQHTGSAFLVAMMTMLRLLPMGLFGAFLGVWAEKMERR
                     TLLVLVVLLMLGTSTCLALLAHGGVLAVWHLAVASFLNGLAWATDNPVRRVMIGEVVV
                     PDQIGAAMSIDVGANNASRMVGPMVGGLLLAGIGLDGIFMLNVALYGFALFAAFRLRY
                     RNTASAGTAPVLARVAEGFRLVRSDRRLLGTLVVTMIYNVFGWPFTSMIPVIG"
     gene            1687..2670
                     /locus_tag="VGC82_03980"
     CDS             1687..2670
                     /locus_tag="VGC82_03980"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY0422463.1"
                     /translation="MATIETALTRLLGIEHPILLAPMGSAAGGKLAAAVTNAGGLGLI
                     GSGYANVDAIRKELTEAGNARVGIGFILWALERNPSALDVALDAKPAAVMLSFGDPTP
                     FTGRIRDAGCKIICQVQTLAQAKEAAVAGADVIIAQGRDAGGHSGTTRGTIGLVPAVV
                     DAVAPIPVVAAGGISDGRGLAAALALGAAGVSMGTRFTACRESLWDQAMKAMTLAAGG
                     DQTEQTRVFDVVRGAPWPAIYPGRALRNAFSARWHGHEEALAADQQGQESAYLATAPD
                     DFATRVVWAGEGVDLINDIPTASQIIERIIAQAAETLMQGSQLVRGIASPG"
     gene            complement(2794..>3952)
                     /locus_tag="VGC82_03985"
     CDS             complement(2794..>3952)
                     /locus_tag="VGC82_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0422464.1"
                     /translation="GYIDNGWFWFGNQGQSYIQLGRFWQIGFFLGLLIWSALVFRALW
                     PTGAAVWQATRQFWSGRIRIENLIWAATLNIAILYVFGMIPLTGIEKSFTIADFWRWW
                     VVHLWVEQSFEFFAASMSAYLLMAVGLVSRKLAERAVYFELILIFLGGVLGTGHHLYW
                     AGGPSMWVPLGSMFSFIEVLPLVLLVIEAIQQYRLIRAQRAFNYTLAYTYLIGAAFWN
                     FVGAGVFGGGTLNAPLINYYEHGTFLTLNHAHTALFGAFGLLAIGLIYFCLRYVAGDR
                     VPFSERLGLWAFWLYNGGLLLWIALNCFPIGWQQLNAVFEHGFAYARSMAFYDTTLIW
                     QWMRLPGDVLFGAAAVLMAWDFLIKLSPLYPRLAERMPGPRGVPLAPRPGE"
BASE COUNT          825 a         1279 c         1243 g          605 t
ORIGIN      
        1 atttttcggc ccggctgatg aattgctcac gtccaaggct gaaagcctgg ccgcctgacg
       61 cgaagcggtc gcagttgcgc ggaggtcgcc tacacggcgg ctatatatgc cgtcgtcgaa
      121 ttggcggcgc acaggaggca atgccgccat ggttgtgctg gccatggcgg ccttaaatat
      181 gccgccacaa cggacgggtc agcgccaacg ccacaaggcc cgccgcaccc gttgccgcgg
      241 ttgcccaatg ggccccgatg gcgtccgcca acatcccgag ggcgataaac cctattggcc
      301 cggcgccgat gcacatcgac agcacgccga ggaggcgcga ccgcatttcg gggggcgacg
      361 cgagaaacac cagcgttgcc tgcatcgtgc tgaagccggc gccacccagg ccggttagca
      421 gcaaggcgcc tccggcaatg gccgggttgg gtgccaaagc gaacacagcc agcattacca
      481 tgtaagccgt cacgccgccg agataagcgc gggcatacca agtcggccgc agggacaacg
      541 ccagcaggat ggcgccgcaa aacgcgccaa tgccgtccat gctggccagg acgccaatcc
      601 cctccggccc gagcccaagc cgcgcctacc cgatcaccgg gatcatgctg gtaaagggcc
      661 agccaaacac gttgtagatc atcgttacaa ccagcgtgcc aaggagccgg cggtcgcttc
      721 gcaccagccg gaagccctcg gcgacgcggg ctagcacggg cgccgttcca gcagaggccg
      781 tgttgcggta gcggaggcga aaagcggcga aaagcgcaaa gccgtacagc gccacgttaa
      841 gcatgaagat accgtcaagg ccgataccgg cgagcaacaa cccaccgacc atcggcccca
      901 ccatgcggct ggcgttgttg gcgcccacgt cgatcgacat ggcggcgcca atctggtcgg
      961 ggacgaccac ctcgccgatc atcacccggc gcaccggatt atcggtagcc caggccaagc
     1021 cattgaggaa actcgctacg gccaaatgcc aaactgccag cacgccgcca tgggccagca
     1081 atgccaggca tgtggatgtc cccaacatga gaagaacaac caggaccagg agggtgcggc
     1141 gctccatctt ctctgcccag acaccaagaa aggcgccgaa cagccccatc ggcagcaacc
     1201 gcagcatggt catcatggcg acgagaaagg ccgacccggt atgctggtaa acgaagacgc
     1261 cgatggcaac cgtctccagc catcgcacgg tgaacacaac caggcctact gaccatagcc
     1321 gccagaaatc cgggcatccc gcgatcagca gttgctgcag cggccgcttg cgcccgagcg
     1381 ggctggctgt ggcgcgcgcc acagcagtga cgccagcgcg cgtcttcaga aagtgtttca
     1441 tgggtttcct ctgcggggat tgcaccacaa gcacctgata agacgccaga tgtggaacag
     1501 ggatgcagct acgcggcggc ggctaccgcg taagcggcga tgatagtgtc atcgtcgcgg
     1561 aaggtgacgt tgttcagaaa tgacggaggc tgaactgatt tggcgcggcg ctaaaagatc
     1621 tatggtttta ccatagccga tggagccatt gctccgatcg gcgccaagga cccgaaagga
     1681 cttcgcatgg ctaccatcga aaccgcgctc acccgcctgc tcggcatcga gcacccaatc
     1741 ctgcttgcgc caatgggctc cgccgctggc ggcaagcttg ccgctgccgt caccaatgcc
     1801 ggcggacttg gcctgattgg ctcgggctat gccaacgtcg atgccatccg caaggaactg
     1861 accgaggctg gcaacgcgcg cgtgggcatc ggcttcattc tttgggcgct ggagcggaac
     1921 ccgtcggcgc tcgacgtggc gctggatgcc aagcccgcgg ccgtgatgct gtcgttcggc
     1981 gaccccacgc cattcactgg ccggatcagg gacgccggct gcaaaatcat ctgccaggtg
     2041 cagaccctgg cgcaggccaa ggaagccgcc gttgcgggcg ccgacgtcat catcgcccaa
     2101 gggcgcgatg ccggtggcca ttcgggaacg acgcgcggca ccatcggtct ggttcccgcg
     2161 gtggtggacg ctgtcgcgcc gatccccgtc gtagcggccg gtggtatttc tgacggacgc
     2221 ggcctggccg cggcgctcgc attgggcgcg gcaggcgtct cgatgggcac ccgcttcacc
     2281 gcctgccgcg agtcgctgtg ggatcaggca atgaaggcca tgacgctggc cgccggcggc
     2341 gatcagaccg agcagacacg cgtgttcgat gtcgtgcgtg gtgcgccctg gccagcgatc
     2401 tatcccggcc gcgcgctgcg caacgcgttc tctgcccgct ggcatggtca cgaggaggcg
     2461 ttggcggccg atcaacaggg ccaggaaagc gcctatctgg caacagcccc ggacgatttc
     2521 gccacgcgtg ttgtctgggc cggcgagggc gtggatctca tcaacgacat tcctacggcg
     2581 tctcagatca tcgagcggat cattgcgcaa gcggcggaga ccctcatgca ggggtcccaa
     2641 ctggtccgcg gaattgcatc gcccggctga cttgagggcc aagacaggtc cggaactgcg
     2701 aggctggtga tcgccggttt tgcacgtaaa ggtgtcggac cgcgtcctcc tcggcgtggc
     2761 gcacggcgtg gtgctcctat gcagtagcag gcatcactca cccggccttg gggccagtgg
     2821 cacgccacgt gggccaggca tgcgttcagc cagccgtgga tacagcggag acagcttgat
     2881 caggaagtcc cacgccatca ataccgccgc cgcgccgaac agcacgtcac cgggcaatcg
     2941 catccattgc cagattagcg tggtgtcata aaaggccatg ctgcgcgcgt aggcgaagcc
     3001 atgctcgaac accgcgttga gttgctgcca gccaatcggg aagcagttca gcgcgatcca
     3061 cagtagcagg ccaccgttgt agagccagaa cgcccacagg ccgagccgtt cgctgaacgg
     3121 cacccgatcg cccgctacat agcgcaggca gaagtagatc aggccgatcg cgagcagccc
     3181 gaaagcccca aacaacgccg tatgggcatg attcagtgtc aggaaggtgc cgtgctcgta
     3241 gtagttgatc agcggcgcat tcagcgtgcc gccgccgaac acgccggcgc cgacgaagtt
     3301 ccagaatgcc gcgccgatga ggtaggtgta ggcgagcgtg tagttgaagg cccgctgcgc
     3361 gcggatcagc cggtattgct ggattgcctc gataaccagc agcaccagcg gcaacacctc
     3421 gataaacgag aacatgctgc caaggggcac ccacatgctg ggtccgccgg cccaataaag
     3481 atgatgtccc gtgccgagca cgccaccgag gaagattagg atcaactcaa aatagaccgc
     3541 ccgttccgcc agcttgcgcg ataccagtcc aaccgccatc agcaggtagg cgctcatgct
     3601 ggcggcgaag aactcgaatg actgctcgac ccacaaatgc accacccacc agcgccagaa
     3661 gtcggcaatg gtgaacgact tttcgatgcc ggtcagcggg atcattccga acacgtagag
     3721 gatcgcgatg ttgagggtgg cggcccagat caggttctcg atccggatgc gtcccgacca
     3781 gaactggcgg gttgcctgcc acaccgcagc gccggtcggc catagcgcgc gaaacaccag
     3841 cgcactccag atcagcaggc cgaggaagaa accgatttgc cagaaccgcc caagctggat
     3901 gtaggactgc ccctggttac cgaaccagaa ccagccattg tcgatatagc cc
//
LOCUS       DASWQN010000242         7644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_949010, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000242 DASWQN010000000
VERSION     DASWQN010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949010"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..1728)
                     /locus_tag="VGC82_03990"
     CDS             complement(265..1728)
                     /locus_tag="VGC82_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0422465.1"
                     /translation="MTQWHRRSATALAVAYARREVSPEDVLQSCLDRLDQINPVLNAV
                     IAQNRAAAKAAASASAARWRAGTPIGALDGIPFTVKDNIVTADLPTTWGSPLFRDYRA
                     KLDELPVARLLQAGAVLIGKTNVPELTSQGYTDNTLFGPTGNPWNPALTPGGSSGGAV
                     AAVAFGIGPVALGTDGGGSIRRPASHAGVVGLKPTPGRVPRCNGLPAILLDFEVIGPI
                     ARTIADLTAVMAAISAPDPRDPASRAFPRFAETAGLAPQRILFVPRFGAHPVDPEIAA
                     SVASAARLFAGMGHQVREADAPFDIEETASIQATISQAGLAWLMRSMNATPSAPSLQD
                     AAAAGAALPAALLFAALDATARLRRSMALFFETTDLILTPSAAALPWPKTETHPPRID
                     GKPAGPRGHAVFTAFANIAALPALALPSAPSFSGLPIGFQVVGSSGSDAALLALGAQY
                     EAAFPWADRWPPTAYSADPGGKARDQPIPGRDQTLIT"
     gene            complement(1725..2132)
                     /locus_tag="VGC82_03995"
     CDS             complement(1725..2132)
                     /locus_tag="VGC82_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0422466.1"
                     /translation="MYLIDTCVVSEARRRTPQAVAWLRAARSETLFLSAITIGEITKG
                     IMMKLRTDPTAATSLLRWLDELRFVYSARILPVDDAVATTWGHLMAQRTRPVADALIA
                     ATAHVNNKIIVTRNVADFADAGVPVLDPWAIAG"
     gene            complement(2132..2401)
                     /locus_tag="VGC82_04000"
     CDS             complement(2132..2401)
                     /locus_tag="VGC82_04000"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY0422467.1"
                     /translation="MQDWQLQDAKNRFSEVVKRARDEGPQTVTVHGQRAAVVVSALQF
                     DALIKPERSFVDFLLAETPGQDPWPDDVVDAINDRSADTGRDIDF"
     gene            complement(2472..2861)
                     /gene="rbfA"
                     /locus_tag="VGC82_04005"
     CDS             complement(2472..2861)
                     /gene="rbfA"
                     /locus_tag="VGC82_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010669055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY0422468.1"
                     /translation="MKKVAKTGPSQRQLRVGEEIRHVLSGIFGRGEFRDPDLATAQIT
                     VTEVRIGPDLKRATVFFSRLGRSDADALVPALARATPYLRGQVAKALRLRIAPDLTFA
                     PDQALDYAIKIDELMHRPEVARDLQEN"
     gene            complement(2858..5581)
                     /gene="infB"
                     /locus_tag="VGC82_04010"
     CDS             complement(2858..5581)
                     /gene="infB"
                     /locus_tag="VGC82_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY0422469.1"
                     /translation="MSESNDQDGGKGRLSLRPAGRLEVGRTVDAGSVRQSFSHGRSKV
                     VQVEVRKKRAPLPNPSAAAGAPPPPPAGAIPAPRAPVTPGRPAPGAGRALTATEQAAR
                     QRALVEQQRDAAKREAERREQEKISILSAAEEARRRETEARKLAEEEARLAEEQAARD
                     KIEAEAKRVAEAAAAANLAARGPSGQDDQSGEPAPQIAAATQSEPRPDQPAAKAPPAR
                     PQPGGQPATPLSATETLRLRAARPEDEEERPVRRPGAGGLPVRKPPVVAPKKGTDDRR
                     RAGRIDVQAAIEGEDEKVRSLASVRRQRERERRQAELDRLRSDQVKVVRDVILPDQIT
                     VQELANRMAARAPEVIKALMKMGVMANITQVLDADTAELVVQEFGHRARRVSESDVEL
                     GLEGIADIDTDMITRPPVVTIMGHVDHGKTSLLDALRATDVAAGEAGGITQHIGAYQV
                     TMAGGEKITFIDTPGHEAFTAMRARGASVTDIVVLVVAADDGVMPQTIEAIRHAKAAN
                     APLIVAINKMDKPDADPTRVRQELLSYEVVVEDMGGETQDVPVSALKRTGLDKLEEAI
                     LLQAELLDLKSNPNRPAEGTVIESRLDRGRGPVGTVLVQRGSLKQGDIVVAGSEWGRV
                     RAMLDDKGKPMVEAGPSSPVEILGLSGAPSAGEPFVVVENESRAREITEFRERRQRDR
                     VAGAAVGARGTLDQMLARIQAGEQKEVAILIKADVQGSAEAIQVVVTKLAHEEVRVRV
                     LLAGVGQITESDVQLAKASSAVVVGFNVRATAQARELAQRDGVDIRYFSIIYEVADDV
                     EKLVKGKVAPKAREKFLGYAEVRKVFNITKTGKVAGCMITEGVVKRGSGVRVLRDNVV
                     IHTGELSQLKRFKDDVREVARGYECGLSFAHFENLQEGDVIECFETEMVPG"
     gene            complement(5584..6261)
                     /locus_tag="VGC82_04015"
     CDS             complement(5584..6261)
                     /locus_tag="VGC82_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HEY0422470.1"
                     /translation="MVTRERLPKERMIRFVVGPDRQIVPDLAARLPGRGIWLSASRDV
                     IESGGAQEERRQKDKQGDGTQRHLARAFARAARGPVSLPPDLSVLLQTALVRRIGDCL
                     GLARRAGQAVAGFEKAREALRTGRYRLVLQASDGSEAERSRFLSGFGPELTIIDPLPG
                     QALGRVFGRDYVVHVAVAPGKLAESLVVEAGRLAGLKNGSARAMGSARATEQETVSVN
                     DVAGTNG"
     gene            complement(6368..>7644)
                     /gene="nusA"
                     /locus_tag="VGC82_04020"
     CDS             complement(6368..>7644)
                     /gene="nusA"
                     /locus_tag="VGC82_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY0422471.1"
                     /translation="LFPDKKVGEFVVDPLPPIDFGRIAAQTAKQVIVQRVREYERKRQ
                     YDEFKDRVGEIINGVVKRTEYGNLMVELGRAEALLRRDELIARESFRNGDRVRAYIYD
                     VREEPRGPQIFLSRTHPGFLAKLFAQEVPEIYDGIIEIKAVSRDPGSRAKMAVISRDS
                     SIDPVGACVGMRGSRVQAVVAELQGEKIDIIPWSSQAATFVVNALAPAEVTKVVLDEE
                     AGRVEVVVPDEQLSLAIGRRGQNVRLASQLTRWDIDILTEAEESERRQDEFRRRSGLF
                     VEALDVDDMIAGLLVTEGFTTVEELAYTPIDEVAAIEGFDESVAEELVRRAEGFLTQR
                     DNALNDQRIELGVSDDVAAFEVLTPPMLVALGEKGVKTLDDLGDLASDELMEIVGADK
                     MDQATANEVIMMARAHWFDGEEQVAAEGAGHD"
BASE COUNT         1293 a         2554 c         2379 g         1418 t
ORIGIN      
        1 cgtcgttttg ccgcagccgg aggggccgag aagcgccacg agttcaccgg gagcgatcgc
       61 gatcgacacg ccatcgacga cgggcgtgtc gccatagcga tggaaaatgc ggtcgagatc
      121 caagcctaca ggagtaggcg caggctcgct cggtttcatg atgcttccat gatcgaaagt
      181 gacagcaagt tatgtgccag accgtgcggg tcgctacgtg gcggaaggca gcgacaccca
      241 gcgtttcgta tgcaagtcga ttggtcaagt gatcagggtt tgatcacgtc caggtattgg
      301 ttgatcgcgt gctttaccgc ctggatctgc cgagtaggca gtgggcggcc agcggtcggc
      361 ccagggaaag gctgcctcat attgcgcgcc caacgccagc aacgcggcat cggaccccga
      421 tgaacccacc acctgaaatc cgatcggcag gccgctgaag gaaggcgcgc tcggaagtgc
      481 caaggccggt agcgcggcga tgttggcgaa cgcggtaaac accgcatggc cacgcggtcc
      541 ggcaggcttg ccgtcgatcc gcggcggatg agtttcggtt ttcggccagg gcaaggcagc
      601 cgccgagggt gtcaggatca gatcggtcgt ctcgaagaac aaagccatcg accggcgcag
      661 gcgtgcggtc gcatcgaggg ccgcgaacaa caaggccgcg ggaagcgccg caccggccgc
      721 tgcggcgtcc tgcaaggatg gagcagacgg cgtggcgttc atcgagcgca tcagccacgc
      781 caggccggcc tgactgatcg tggcctggat ggatgcggtt tcttcgatgt cgaacggtgc
      841 atccgcttca cggacctggt gtcccatccc ggcaaacagg cgcgcagcgc tggcaacgct
      901 ggcggcgatt tcgggatcga ccggatgggc tccgaagcgg ggcacgaaca ggatgcgctg
      961 tggcgccagc ccagcggttt cggcaaatcg cggaaacgcg cgcgaggctg gatcgcgcgg
     1021 atcgggtgcc gaaatggctg ccatgacggc cgtcagatcc gcgatcgtgc gagcgatagg
     1081 gccgataacc tcaaaatcga gcaggatcgc cggaagcccg ttgcagcggg ggacacgtcc
     1141 cggcgtcggc ttcaacccaa ccacgccggc atgtgacgcc ggccgcctga ttgagccgcc
     1201 gccatcggta cccagcgcaa cgggtcctat gccgaaagcg acggcagcga ccgctccgcc
     1261 ggacgatccc cccggcgtca aggccgggtt ccacgggttg ccggtgggtc cgaacaacgt
     1321 gttgtcggtg tagccttggg acgtcagttc gggaacgttg gtcttgccga tcagcaccgc
     1381 tcctgcctga agcagacggg cgaccggcag ttcgtcaagt ttggcgcgat agtcacgaaa
     1441 caacgggctg ccccaggtgg tcggcagatc ggcggtgacg atgttgtcct tgacggtgaa
     1501 gggaatgccg tccagcgcac cgatcggggt gccggcccgc caacgcgcgg cgctggcgga
     1561 tgcggctgct ttcgcggcgg ctcggttttg agcaatgacc gcgttgagca ctgggttgat
     1621 ttggtccaaa cgatcaaggc aagactggag aacgtcttcc ggcgatacct cccgacgggc
     1681 ataagcgact gccagcgcgg tagcggacct acgatgccat tgcgtcatcc cgcgattgcc
     1741 cagggatcga ggaccggcac gcctgcatcg gcgaaatcag ccacgttgcg ggtgacaatg
     1801 atcttgttgt tcacatgcgc tgtcgcggcg atcagcgcat ccgcgacggg acgcgtccgt
     1861 tgggccatca gatgccccca ggtggtggca accgcatcgt ccaccggcag gatgcgggcg
     1921 gaatagacga agcgcagctc atccagccag cgaagcaacg aagttgccgc cgtgggatca
     1981 gtccgcagct tcatcatgat gcccttggtg atctcaccga tggtgatggc gctcaggaac
     2041 aacgtttccg accgggcggc ccgtagccac gccaccgcct gcggcgtacg gcggcgcgct
     2101 tccgacacca cacaggtgtc gatgaggtac atcagaaatc gatatcgcgg ccggtgtcgg
     2161 cgctgcggtc gttgatcgca tcgaccacat catcgggcca agggtcctgg ccgggggttt
     2221 cggccagcag gaaatccacg aaggatcgtt ccggcttgat cagcgcgtcg aactggagtg
     2281 cggaaacaac caccgccgcg cgctgaccat gcacggtcac ggtctgggga ccctcgtccc
     2341 gagcccgctt gaccacttcg ctaaagcgat ttttagcgtc ctgcaattgc cagtcctgca
     2401 ccgccgcccc ctgatctgtc cagacaggct agattaccgg tccaaatcga ttgcgtcaat
     2461 ctaatatcat atcagttttc ctgcaggtcg cgggcgacct cggggcggtg catcagttcg
     2521 tcgatcttta tcgcgtagtc cagtgcctgg tcgggcgcga acgtcaggtc cggggcgatg
     2581 cggagcctga gggccttggc gacctgacca cgcaggtaag gcgtcgcgcg ggccagcgcc
     2641 gggaccagcg catcggcatc cgaccgaccc agccgcgaga agaacaccgt cgcccgcttc
     2701 aggtccggac cgatccggac ctcggtaacg gtgatttgcg cggtcgcgag atcgggatca
     2761 cggaattccc cccgcccgaa gatcccggac agaacgtgcc gaatttcctc ccctacccgc
     2821 aactgacgtt gcgacgggcc cgtcttggct actttcttca acccggcacc atctccgttt
     2881 caaagcactc gatgacatcg ccctcctgca ggttctcgaa gtgagcgaac gacagaccac
     2941 actcgtagcc gcgcgccact tcgcggacgt cgtccttgaa gcgcttgagt tgcgagagct
     3001 cgccggtgtg gatcaccacg ttgtcacgca gcacgcgcac gccggaacca cgcttcacga
     3061 cgccttcggt gatcatgcag cctgcgacct tgcccgtctt ggtgatgttg aacaccttcc
     3121 ggacctcggc gtagccaagg aacttttcgc gggctttggg ggccaccttg cccttaacca
     3181 gcttttcgac gtcgtcggcc acttcataga tgatcgagaa gtagcggata tccacgccgt
     3241 cacgctgagc gagttccctc gcctgtgctg tcgcacgcac attgaagccg accacgacgg
     3301 cgctggaagc cttcgcgagc tgcacgtcgc tttccgtaat ctgcccgacg ccggccagca
     3361 gcacgcgcac cctgacttcc tcatgcgcca gcttcgtcac caccacctgg atggcttcag
     3421 cgcttccctg cacgtcggcc ttgatcagga tcgcgacttc cttctgctcg cccgcctgaa
     3481 tacgagccag catctgatcc aatgtaccgc gcgctccgac cgctgcccct gcgaccctgt
     3541 cgcgctgccg gcgttcacgg aattcggtga tctcccgcgc ccggctttcg ttctcgacga
     3601 ccacgaacgg ttcgccggcg gagggtgcgc cgctcaaacc caggatttcg accgggctgg
     3661 acggtccggc ttcgaccatt ggtttgcctt tgtcgtccag catggcgcgc acgcggcccc
     3721 attcggatcc tgcaaccacg atgtcgccct gcttaaggct gccgcgctgt accagcacgg
     3781 tcccgactgg gccgcggcca cggtcgagac ggctttcgat aaccgtgccc tcggccggcc
     3841 ggttcggatt ggacttcagg tccagcagtt ccgcttgcag cagaatcgcc tcctccagct
     3901 tgtccagtcc agtccgcttc agggccgaaa ccggcacgtc ctgcgtttca ccgcccatat
     3961 cctcgacaac cacctcatag ctgaggagtt cctggcgcac gcgggtcggg tcggcatcag
     4021 gcttgtccat cttgttgatg gcaacgatca gcggagcgtt ggccgccttg gcgtggcgaa
     4081 tcgcctcaat cgtctgcggc atgacgccgt catcggccgc caccaccagc accacgatat
     4141 cagtgaccga cgcaccacgt gcccgcatgg cggtgaacgc ttcatggccg ggggtgtcga
     4201 tgaaggtgat tttctcgccg ccggccatcg tcacctgata ggcgccgatg tgctgggtga
     4261 tcccgccagc ctcgcccgcc gccacatcgg tggcccgcag cgcatccagc aacgaggtct
     4321 tgccatgatc gacatggccc atgatcgtca cgaccggcgg gcgggtaatc atgtcggtat
     4381 cgatgtcggc aatgccctcg aggccgagct cgacgtcgga ttccgacacc cggcgcgcgc
     4441 ggtggccaaa ttcctgcacc accagttcgg cggtgtcggc atccagaacc tgggtgatgt
     4501 tcgccatcac acccatcttc atcaacgcct tgatgacctc gggtgcgcgg gcggccattc
     4561 gatttgccag ttcctgcacc gtgatctggt cgggcaggat aacatcgcgc acaaccttca
     4621 cctggtcgga gcgtagcctg tccagttccg cctgccggcg ttcacgttcg cgttggcgac
     4681 ggaccgaggc cagcgagcgg actttttcgt cctctccctc gatcgctgcc tgcacgtcga
     4741 tgcgacccgc gcggcgacgg tcatcggtgc ccttcttcgg cgcaaccacc ggcggcttgc
     4801 gcaccgggag ccctccggca ccgggccggc gaaccgggcg ttcttcctcg tcttccggac
     4861 gcgcggcacg cagccgcagc gtttcagttg ccgacaaggg tgtggccggc tggccgccgg
     4921 gttgtggcct ggccggcggt gccttggcag ccggttggtc cggccgcggc tctgattgcg
     4981 ttgccgcagc gatttgcggc gcaggttcgc cggactggtc gtcttgtccg gatggaccgc
     5041 gtgcagccaa gttcgcggct gcggcggctt ccgcgacgcg cttcgcttca gcctcgatct
     5101 tgtcgcgagc agcctgttcc tcggccaacc gagcttcttc ctcggcgagc ttacgcgctt
     5161 cggtttcgcg gcggcgcgct tcctcggcgg cggaaaggat cgaaatcttc tcctgctcgc
     5221 ggcgttcggc ttcgcgcttg gcagcgtccc gctgctgttc gaccagcgcg cgctgccggg
     5281 cggcctgttc cgtcgccgtc aatgctcgtc cggcgccggg ggccgggcgg ccaggcgtta
     5341 ccggtgcccg aggtgcggga atcgcaccgg cgggcggtgg cggcggtgcg cctgcggcgg
     5401 ccgagggatt aggcagcggc gcacgcttct tgcgcacctc gacctgcacg accttcgacc
     5461 ggccgtggct gaagctttgg cggacggaac ccgcatcgac ggtccgtccc acctccagac
     5521 gtcctgccgg acgcagcgag agccgccctt tgccgccgtc ctgatcgttg ctttcgctca
     5581 tgtttatccg ttagtacccg ctacatcgtt caccgaaaca gtttcctgtt ccgtggccct
     5641 tgcggacccc atggcccttg cggacccatt cttcaatccg gccagcctgc ccgcttccac
     5701 gacgagcgac tctgccaatt tgcccggcgc gaccgcgacg tgcactacat agtctcgacc
     5761 gaacacgcgt cccagggcct gacccggaag tggatcgata atcgtcagct ccggaccgaa
     5821 gccggacagg aagcgcgaac gctccgcctc actaccgtcc gaggcttgca gcaccagccg
     5881 atagcggcca gtccttagcg cctcacgcgc cttttcgaaa cccgccacgg cctgtccggc
     5941 gcgtcgggcc agaccgagac agtcgccaat ccgacggacc agcgccgtct gaagcagaac
     6001 agaaagatcc ggtggcagcg atacggggcc gcgtgcggcc ctggcaaatg cgcgtgcgag
     6061 gtggcgttgc gtgccgtccc cctgcttgtc cttttgccgc ctttcttcct gggcgccacc
     6121 agactctatc acatcccgcg acgcgctcaa ccagattccc cgtccgggca gccttgcagc
     6181 caggtcgggg acgatctgcc gatccggccc gacgacgaag cggatcatgc gttccttcgg
     6241 caagcgttca cgtgtaacga tgcagcggcg aagcggcccg gtttccggct ccggcgcggc
     6301 gtcgagcgcg tggtcgtcag cgtcaacgga ctccggatca ggaacgtcgg tgccgggggt
     6361 gtcagattca gtcgtgaccc gccccctccg ccgcgacctg ttcctcacca tcgaaccaat
     6421 gagcacgcgc catcatgatc acttcgttgg cggtcgcttg atccattttg tccgcaccga
     6481 cgatctccat caattcgtcc gaggcgaggt cgcccagatc gtcgagagtc ttaactccct
     6541 tttcaccgag ggcaaccagc attggcggcg tcagcacctc gaaagcggcg acgtcgtcgc
     6601 tgacgccgag ttcgatgcgc tggtcgttca gggcgttgtc acgctgcgtc aggaacccct
     6661 cggctcgccg caccaactcc tcggcgacgc tttcgtcgaa gccctcgatc gccgcgactt
     6721 cgtcgatcgg ggtataggcc aactcctcga ccgtggtgaa gccttcggtc accagaaggc
     6781 cggcgatcat gtcgtccaca tccagcgctt cgacgaacaa cccggagcgg cggcggaatt
     6841 catcctggcg ccgttcgctt tcctcggctt cggtcaggat gtcgatgtcc cagcgggtga
     6901 gctgagacgc cagccgcacg ttctggccgc ggcggccgat ggcgagcgaa agctgctcgt
     6961 ccggcaccac gacttccacc cggccagcct cttcatccag aacgaccttg gtcacctcgg
     7021 ccggagcgag cgcattgacc acgaaggtgg cggcctggct gctccaggga atgatgtcga
     7081 tcttttcgcc ctgcagctcc gccaccactg cctgcacgcg cgagcctcgc ataccgacgc
     7141 aggcgcctac cggatcaatc gaactgtcac ggctgatcac cgccattttc gctcggctgc
     7201 ctggatcgcg tgataccgct ttgatctcga tgatgccgtc gtagatttcc ggcacttcct
     7261 gcgcaaacaa cttggccagg aagccaggat gggtgcgcga gagaaagatc tgcggaccgc
     7321 gaggttcttc gcgcacatcg tagatatagg cccgtacccg gtcgccattg cgaaagcttt
     7381 cgcgggcaat gagttcatcg cgacggagca gcgcctccgc ccggccgagt tcgaccatca
     7441 ggttgccgta ctcggtacgc ttcaccacgc cgttgatgat ttcgcccacg cggtccttga
     7501 attcgtcgta ttgacgcttg cgttcatatt cgcgaacccg ctggacgatc acctgcttgg
     7561 cggtttgtgc cgcgatgcgg ccaaaatcga tcggcggcag aggatcgacg acgaactcac
     7621 cgaccttctt atccgggaac agct
//
LOCUS       DASWQN010000243         1903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_818062, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000243 DASWQN010000000
VERSION     DASWQN010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818062"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VGC82_04025"
     CDS             <1..299
                     /locus_tag="VGC82_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422472.1"
                     /translation="AVLLDLCLPDIDGFDVIDRLRADPATADIPVIVCTSSVLAAHQR
                     SRLNHARAILSKANLTRETMHRALVDSGLTDSGLTDSELSDSGLTDTPIGLSDE"
     gene            296..>1903
                     /locus_tag="VGC82_04030"
     CDS             296..>1903
                     /locus_tag="VGC82_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0422473.1"
                     /translation="MSATTATRPLPLVLNVDDDVANRYAKTRSLQMGQIEVIEAGDGT
                     SALALIAERQPLLVLLDVNMPDMDGREVCRHIKVKWPDIIVIQVSATRISVADKIIGL
                     ENGADCYLTTPLDPWELVAVTRAMLRLRRAETQAQEADERYRMIVESAVDYAIFTCGL
                     DGRITSWNSGAQAILGYAPEEIIAQQAARIFMDEDIAEGVPEAEMRAARQGLSIRAER
                     WHRRRDGTRFWSSGRLVALRDRRGQVAGYLKILYDRSFEKQARDALEALNLELEERVA
                     ERTRELAEANDRLRLEMAERAHAAEEIRQLQKMEALGHLTGGIAHDFNNLLTAILGGL
                     EITRRRIEDPRSLKLIDSSISAAQRGAKLIAQLMAFARKQNLQVEYLPLNTLVEEMQE
                     LLGRSVGAAVRLIYDFQPDVWPVMADASQVQTALLNLSINARDAMPGGGILRIATGNV
                     HVHFAEADLAAGDYAALTVQDNGSGMTDEVKSRLFEPFFTTKGVGKGTGLGLAQVHGF
                     VHQSGGTVRVHSVLGEGTKFTILLPRAA"
BASE COUNT          366 a          596 c          619 g          322 t
ORIGIN      
        1 atgcggtgct gctggacctt tgcctccccg atatcgacgg cttcgacgtg atcgaccgcc
       61 tgcgcgccga tccggcgact gccgatatcc cggtgatcgt ttgcacctcc tcggtcctgg
      121 cggcccatca gcgcagccgg ctgaaccatg cgcgagcaat cctgtccaag gcgaatttga
      181 cgcgcgaaac gatgcaccgc gcgcttgttg actcggggct gaccgactcg gggctgaccg
      241 actcggagct gagtgactcg gggctgaccg acacgcccat cggcctgagc gacgaatgag
      301 tgccacgacc gccacccggc ctctcccgct ggtcctgaac gtggacgacg acgtggcgaa
      361 ccgctacgcc aagacgcggt cgctgcagat gggccagatc gaggtgatcg aggccggcga
      421 cggcacttcc gccctcgcgc tgattgctga acgccagccg ctattggtcc tgttggacgt
      481 caacatgcct gacatggacg ggcgggaagt ctgccgtcac atcaaggtaa aatggccgga
      541 tatcatcgtg atccaggtct cggccacccg tatttccgtt gccgacaaga tcatcggcct
      601 ggaaaacggt gcggactgct acctgacgac gccgctcgat ccttgggaac tggtcgcggt
      661 tacccgcgcg atgctgcgcc tgcgaagggc cgaaacgcag gcacaggaag ccgacgaacg
      721 ctatcgcatg atcgtcgaga gcgcggttga ctacgcaatc ttcacctgcg gcctggatgg
      781 ccggatcacc agttggaact ccggcgccca ggctattctt ggctatgcgc ccgaggagat
      841 catcgcccag caagctgccc gcatcttcat ggacgaggat atcgccgaag gggttcccga
      901 ggccgagatg cgggcagctc gccagggtct cagcatacga gccgaacgct ggcaccgccg
      961 ccgcgacggc acgcgcttct ggtccagcgg tcgcctggtg gcgttgcgcg accgccgcgg
     1021 gcaagtcgcc ggctacctca agatcctcta cgaccgctcc ttcgagaaac aggctcggga
     1081 tgcgctggaa gcgctcaacc tggaactgga ggaacgtgtc gccgaacgga cccgagaatt
     1141 ggcggaggcc aacgacaggc tgcgtttgga gatggccgaa cgggcgcacg ccgcggagga
     1201 aatccgccaa ctgcaaaaga tggaggcgtt gggccatctc acgggtggca tcgcgcatga
     1261 cttcaacaac ctgctgaccg cgatcctggg tgggctggaa atcacccgcc ggcggatcga
     1321 agaccccaga agcctgaaat tgatcgacag ttcgatcagc gcggcccagc gcggtgcgaa
     1381 actgatcgcg caactcatgg cgttcgcccg caagcagaac ctgcaggtcg aatacctgcc
     1441 gctgaacacg ctggtcgagg aaatgcagga actgctgggg cgcagcgtcg gggcagccgt
     1501 ccgcttgatc tatgattttc aaccggatgt gtggccggtc atggccgatg ccagtcaggt
     1561 gcagacggcg ttgctgaacc tgtcgatcaa tgcccgggac gcgatgcccg gcggtggaat
     1621 cctgcggatc gcgaccggca atgtccatgt tcacttcgcc gaagccgatc tggccgctgg
     1681 ggattatgcg gcgctgacgg tccaggataa cggctccggc atgacggacg aggtaaaatc
     1741 ccggttgttt gagccgttct tcaccaccaa gggcgtcgga aaaggcaccg gcctgggatt
     1801 ggcccaggtc catggcttcg tccaccagtc gggcggaacc gtgcgggtgc acagcgtgct
     1861 gggcgaaggg acgaaattca cgatcctgct gccgcgcgcg gca
//
LOCUS       DASWQN010000244         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1081277, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000244 DASWQN010000000
VERSION     DASWQN010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081277"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VGC82_04035"
     CDS             <1..352
                     /locus_tag="VGC82_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422474.1"
                     /translation="YVCDWGNDEQPYKMTPKTGELYSLGVNAYLDDNYIHLHGRRTIN
                     EVNLLWREWFDGLYADGTTTGRLMVLHLHPWIMGQPWRIRHLDEVLGHISAHRGVWKA
                     TGGAIIDWFKAQSN"
     gene            405..1298
                     /locus_tag="VGC82_04040"
     CDS             405..1298
                     /locus_tag="VGC82_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0422475.1"
                     /translation="MTLAPGRFDYAPITERPVIKWPGNARVAFWVAPNMEFFEYLPEN
                     RPTQPDIPHYARMDYGNRVGFWRMLDVLNKNKVRACCCLNLELLDHFPEIKDAMVAND
                     WDYMAHGLYNSRPIYDYTEEQERAYWQDFITHVKAMTGKQVKGRLGGGAGYTIRTDDL
                     MAEAGCLYHTSWIMDDQPWPINVRGGQKFIYVPYTGQTNDAGMLSWNREADYFLQMIK
                     DQFDTLYREGADNGRVMCLSLHPHNIGRPNAVKYLDQALQYILGHDGVWNTTADDIAE
                     YYIANYYNQVSSWIAESKARA"
     gene            1507..>2206
                     /locus_tag="VGC82_04045"
     CDS             1507..>2206
                     /locus_tag="VGC82_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY0422476.1"
                     /translation="MTDEKRWSDRALGMNRSIQRRDFLNGVAIAIGAGLIGSRSAAAL
                     AGESWPQDQPGYYPPLLIGLRGSHPGSFETAHALRNADFWQNHPGIKETGERYDLVVV
                     GGGISGLSAAHFYRAQRPDAKILILDNHDDFGGHAKRNEFHLGGKLHLLNGGTLEIDS
                     PRPYSPVADGLLKALGVDPVALTKACNRDGLYPSLGLSRAIFFDKETFGEDRLVNGLP
                     GRQQKASAGIWRTFL"
BASE COUNT          480 a          670 c          652 g          404 t
ORIGIN      
        1 ctacgtctgc gactggggca acgacgagca gccctacaag atgacgccca agaccggtga
       61 gctctactcg ctcggcgtga acgcctacct cgacgacaac tacatccacc tccacggccg
      121 ccggaccatt aacgaggtca atttgctatg gcgtgagtgg ttcgatggtc tgtacgccga
      181 tggcacgacc accggacgac tgatggtgct gcatctgcat ccatggatca tgggccagcc
      241 ctggcgcatc cggcacctgg atgaagtgct tggccacatc agtgcccacc ggggcgtctg
      301 gaaggcgacc gggggtgcca tcatcgactg gttcaaggcg cagtcgaact agctgccccc
      361 ttcaaggccg aattcaggtc catgcagacg acggagaaca cgacatgact ttggcacccg
      421 gtcgcttcga ctacgcaccc atcactgaac ggccggtcat caagtggccc ggcaacgctc
      481 gcgtcgcctt ctgggtcgcg ccgaacatgg agttcttcga atacttgccg gagaaccggc
      541 cgacgcaacc ggacattcca cattatgcgc gcatggatta cggcaaccgc gtcggcttct
      601 ggcgcatgct cgacgtgctg aacaagaata aagtgcgggc atgctgctgc ctgaaccttg
      661 agctcctcga tcatttcccc gaaatcaaag acgccatggt cgccaacgac tgggactaca
      721 tggcgcacgg cctctacaac agccgaccga tttatgacta caccgaagag caggaacggg
      781 cctattggca ggatttcatc acccatgtga aggcgatgac cggcaaacag gtgaaaggcc
      841 gccttggcgg cggcgcgggc tataccatca ggacagacga cctaatggca gaagcaggct
      901 gcctctatca cacgtcctgg atcatggatg atcaaccctg gccgatcaac gtgcggggcg
      961 gccagaagtt tatctatgtg ccctataccg gacagacaaa tgatgccggc atgttgagct
     1021 ggaatcggga ggcggattac tttctgcaga tgatcaagga ccagttcgat acgctttacc
     1081 gtgaaggtgc ggacaatgga cgtgtaatgt gtctttcgct tcatccgcac aatatcgggc
     1141 gtccgaacgc tgtaaagtac ctggaccaag cactgcagta catcctcggc cacgacggcg
     1201 tttggaacac gaccgccgac gatatcgccg aatactacat tgccaactac tacaaccagg
     1261 tgtcatcctg gatcgccgag agcaaagctc gggcataagc gcgggccgga ggagtctccg
     1321 gcaatccccg cgagcatcat tcgcgtggac gagcaatgaa cacttccgcg atagcggatg
     1381 tggcggctcg cgatccggca tcacgaggca atgccagaat cagccggtca acagcggcgg
     1441 tgacacttga cgaacattac ccgaatggag cggtgtaccg caacggcttt acggttcggg
     1501 aaaaagatga ccgacgagaa gcgttggagc gaccgcgcgc tgggtatgaa ccgctcgatc
     1561 caacggcgcg atttcctcaa tggcgtagcg atcgcaatcg gcgccggcct gatcggctct
     1621 aggtctgcgg ccgcactggc cggtgaaagc tggccgcagg accagccggg ttattatccg
     1681 ccgctgctga tcgggctacg tggcagccat ccaggctcgt ttgagaccgc ccatgcccta
     1741 cgcaacgctg atttctggca gaatcacccg gggatcaagg agaccgggga gcgctatgac
     1801 cttgtcgttg tcggcggcgg gatcagcgga ctttccgctg cccacttcta ccgcgcccaa
     1861 cggccggacg cgaagatcct gatcctggac aaccacgatg acttcggcgg ccacgccaag
     1921 cgcaacgagt tccatttggg cggcaagctg caccttctga atggcggcac actggagatc
     1981 gacagtccgc gcccctacag ccccgtggcc gacggcttgc tgaaagctct gggcgtggat
     2041 ccggtggcgt tgaccaaggc ctgcaaccgc gacgggctct acccttcgct cggtctatct
     2101 cgggcaatct tcttcgacaa ggagaccttc ggcgaggacc ggctggtgaa cggcctgcct
     2161 gggcggcagc aaaaagccag cgctggtatt tggcgcacct ttcttg
//
LOCUS       DASWQN010000245         3863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2269344, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000245 DASWQN010000000
VERSION     DASWQN010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2269344"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..685)
                     /locus_tag="VGC82_04050"
     CDS             complement(269..685)
                     /locus_tag="VGC82_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HEY0422477.1"
                     /translation="MLTRRLVLSSTLLLPTAAFARTTAPKDAYLYIIWPTDGTHVKGA
                     FWCRFGLRDMGITHAGDATPNMGHHHLLIDVDETLDVNVPIPSDKKHLHYGAGQTEAR
                     LDLPPGPHTLQLVLGDTEHIPFDPPVVSKKIRIIVS"
     gene            complement(702..2237)
                     /locus_tag="VGC82_04055"
     CDS             complement(702..2237)
                     /locus_tag="VGC82_04055"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY0422478.1"
                     /translation="MTEEVTRRLAAIVMADVVGYSRLMNADERGTHQRVQRLLRELIE
                     PTIVEHRGRLVKQRGDGFLSMFDSPVEAVRCAIIIQQSMVSRNLEVPNEQAIRFRIGV
                     NLGDVIVVADDIYGEGVNVAARLEQLAEPGNIYISGGVYEQVRYKLVCGYQSLGDQKV
                     KNILDPIATYRVLPDPDSVRKATRRPGYKVLAYGLMGGISISLALGALYFWHTMSTPE
                     QHSVAVTNLAVEPIAPAVAAKPIIAEKPVARESEPAASPAPAQIQEAAAAVPLRPQIE
                     APPAAPAPAPPPVGEPEMLAIPGGTFQMGSNEDASEQPIHRVTVKPFLLAKYPVTVRQ
                     WLECSRAKACAYTPSGNADAPVSNVSWADAQQYVTWLSQTTHHRYRLPTEAEWEYAVR
                     GGTDTRYWWGNTMKPGFAGCKGCGDSAPPVKVGTRVPNPFGVFDINGGVTEWMEDCWF
                     KDYRGAPADGSARLSASACREHVLRGGSWSNDQNYVRSASRDFYDTSVRYPTHGIRVA
                     RSP"
     gene            2313..3020
                     /locus_tag="VGC82_04060"
     CDS             2313..3020
                     /locus_tag="VGC82_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0422479.1"
                     /translation="MANSPLSREEMLSGHFLLRHLHPDELSRVAASAKLLRYKPQTTI
                     FQKGDPGASMMAVVTGRVKICTFSLEGKEMVLNYIDRGGLFGEIALLDGQPRSADAVA
                     IEDTELLVLERNRVMPILSANPQIAAQVITVLCQRVRQASEALEDALLRDAPSRVARG
                     LLRLARTFGKPTGKGGLKLDIKLSQQQLGSLIGITRESTNRCIVEWSRAGYLAVDAGF
                     ITILDREALESLSEAEV"
     gene            3127..3399
                     /locus_tag="VGC82_04065"
     CDS             3127..3399
                     /locus_tag="VGC82_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422480.1"
                     /translation="MQTMRKTLSSGLATLAVVGFVSAAGAAEPSKQKTDGNSPTTVGP
                     GSAAYKQKTDGNSPTMVGPGSGAYKQRTDGDSPTTVGPGSRAFKQQ"
     gene            complement(3529..>3863)
                     /locus_tag="VGC82_04070"
     CDS             complement(3529..>3863)
                     /locus_tag="VGC82_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEY0422481.1"
                     /translation="MGGYGLSTERMAFYWNVYVPHEIDRLHPLAAPLRADLIGLPPVM
                     VLLAELDVLRSEGEALVAKLRSSGVPVESETFGGTVHGFLRATGSVQKARDAVALAGS
                     WLRRLAEA"
BASE COUNT          771 a         1157 c         1151 g          784 t
ORIGIN      
        1 gtccgtgcaa catcacccaa gcgggatcgt gaagcccgtt agaccatttt cagcctgact
       61 gtgcaggccg caaatgctct gccccctgtt cgatcgcgtg actcacacgc ttcgacgttc
      121 cgcccatcgt gatcacgctc caatgtccca aatctgaggt ccgccatatc cggcgaacct
      181 cagtctcgcg ccactctctc tttgtcttca gtggtctgct gatcccctgc gtccgccgca
      241 tgatgcgacg aacttcgggg gcaggacact agctgacaat aattcggatt ttcttcgaga
      301 caacaggagg atcgaacggg atatgttctg tatcgcctag tacaagttgc agcgtatgcg
      361 gtccgggcgg caggtcgaga cgagcttcgg tttgccccgc gccgtaatgc aaatgcttct
      421 tgtctgatgg aatcggaacg ttgacatcaa gcgtctcatc cacgtcgatc agcagatgat
      481 gatgacccat gtttggcgtc gcatccccag cgtgggtgat ccccatatcg cgcagcccaa
      541 aacgacacca gaaggcgccc ttgacgtggg tgccgtcagt tggccagatg atatagaggt
      601 acgcgtcctt aggagccgtg gttctggcaa aagccgcggt gggcagcagc agcgtcgatg
      661 ataataccag tcgtcgggtc aacatccgtt tactccgggt ttcaaggtga gcgggcgaca
      721 cggataccgt gagtcggata gcggaccgac gtgtcataaa agtcccggct ggcagagcgg
      781 acgtaattct ggtcgttgct ccatgatcca ccgcgtaaga cgtgttcccg gcacgctgaa
      841 gctgataggc gtgccgatcc gtcggccggt gcgccgcggt aatccttgaa ccagcaatct
      901 tccatccatt cggtgacgcc gccattgatg tcgaacacgc cgaatgggtt gggcacccgc
      961 gtcccaacct ttaccggcgg ggcagaatca ccgcatcctt tgcagccggc gaaacccggc
     1021 ttcatcgtat tgccccacca gtaccgcgta tccgtgccac cacgcaccgc gtattcccac
     1081 tcggcttcag tcgggagacg gtaccgatga tgtgtggtct gcgacaacca tgtgacgtat
     1141 tgctgggcat cggcccaact gacgttgctc acaggtgcgt cggcattccc gctcggggta
     1201 taggcgcagg ctttcgctcg actgcattcc aaccattggc gcacagttac ggggtacttc
     1261 gccagcagga atggtttcac ggtcactcgg tggatcggct gttccgatgc gtcttcattg
     1321 ctgcccattt ggaaagtccc tcccgggatg gcaagcatct cgggttcgcc aaccggcggc
     1381 ggcgcgggag ccggcgcggc cggaggcgcc tcgatttgcg ggcggagcgg aaccgcggcg
     1441 gcagcctctt gaatctgggc cggagcaggg gaggcggcgg gttcagactc gcgggctacc
     1501 ggtttttccg cgattatggg ttttgcagcg actgccggcg caatgggttc gaccgctaga
     1561 ttcgttaccg caacgctatg ctgttcgggc gtcgacatcg tgtgccagaa atacaatgca
     1621 ccaagcgcga gactgatgct tataccgccc atcaagccgt aggccagcac tttgtagccg
     1681 ggcctgcgcg tggctttgcg caccgaatcg ggatcgggca ggactcgata cgtcgcgatc
     1741 gggtcgagga tattcttgac tttttggtcg cccagcgact ggtagccgca gaccaacttg
     1801 tagcgtacct gctcatagac gccgccggag atgtatatgt tgccgggttc ggcgagttgt
     1861 tccaaccgag cggcgacgtt gaccccttcg ccataaatat cgtccgctac tacgatcacg
     1921 tcgccgagat tgaccccaat gcggaaccgg atggcctgtt cattcggtac ttcaaggttg
     1981 cggctgacca tgctctgctg gatgatgatg gcacagcgca cggcctcgac cgggctgtcg
     2041 aacatcgaca gaaagccatc gccgcgctgt ttcaccagcc ggccccgatg ttccacaatg
     2101 gttggctcga tcagttcacg cagcaatcgc tgaacccgtt ggtgggtacc acgctcgtcg
     2161 gcattcataa ggcgtgaata gccaacgaca tcggccatta cgatcgcagc gagtcgtctg
     2221 gtgacttcct ccgtcatggg tagcctcgac ttgagatcat ggaccggctc aaatatttga
     2281 actattgtag ctcaaatact ggatcgggcg aaatggcgaa ttctccattg agccgggaag
     2341 aaatgctttc cggccatttc ctgttgcggc atctgcatcc ggatgagctg tcgcgggtcg
     2401 cggccagtgc caaattgttg cgctacaagc cgcagacgac gatcttccag aaaggcgatc
     2461 ccggcgcgag catgatggca gtcgtgaccg gtcgcgtgaa aatttgcacc ttctcgttgg
     2521 aaggtaaaga aatggtgttg aactatatcg atcgcggcgg cctgttcggg gaaatcgcct
     2581 tgcttgacgg tcagccacgt tcggccgacg ctgtcgcgat cgaggatacc gagctcctgg
     2641 tgctggaacg caaccgcgtg atgccgatcc tgagcgccaa tccccagatc gccgcccagg
     2701 tgatcacggt gctctgtcag cgcgtacgcc aggctagtga agcgctggaa gatgcgctgc
     2761 tacgggacgc gccatcacgc gtcgcccggg gtctgttgcg gttggctcgg acgttcggta
     2821 agcccacggg gaaaggcggc ttgaaattgg atatcaagct gtcgcagcag caactcggca
     2881 gcctgattgg catcacgcgt gaaagcacca acaggtgcat cgttgagtgg agccgagccg
     2941 gttaccttgc ggtcgacgcc ggtttcataa ccatattgga tcgcgaagcg ctggaaagcc
     3001 tatctgaagc cgaggtgtag gaaagacgat tccctgtgat gtgtttcaca gcgccggcca
     3061 gaggtgctac gcaatactgc ggcccgggac gagcaatcgt gctcgatccc aaacaggaat
     3121 tgcatcatgc aaaccatgag gaaaacgctc agttctggtc tggcgacgtt ggcggtcgtc
     3181 ggcttcgtct ccgcggccgg tgcggccgaa ccgtccaagc agaaaacaga cggaaatagc
     3241 ccgactacgg ttgggcccgg aagtgctgcg tacaagcaga agaccgatgg caacagccca
     3301 accatggtcg gacccggcag cggcgcttac aagcagcgga cagacggcga cagtccaacc
     3361 acggtcggac caggcagccg tgctttcaag cagcagtagc cagttccggg cggggaaggg
     3421 aacttccccg ttccgctcgt ctgttatggc tggaagcgta gccctaatac agccggctag
     3481 tgctgcaatc gttggttcgt agtacggcgg ctattccgcc caacaggatc atgcttcggc
     3541 cagccgcctc aaccagctcc ctgccaacgc gaccgcgtct ctggctttct gcaccgatcc
     3601 agtcgcgcgc aggaacccat gcacggttcc gccgaacgtt tccgactcca ccggcacgcc
     3661 cgaggagcgg agtttcgcca ccagcgcctc accttcggaa cgcaggacgt cgagttccgc
     3721 cagcaatacc atgaccggcg gcaggccgat caggtccgcc cgcagcggtg ccgccagcgg
     3781 atgcagccgg tcaatttcgt gcggcacata aacattccag tagaacgcca tccgttcggt
     3841 gctgagcccg tacccgccca tcc
//
LOCUS       DASWQN010000246         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1609490, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000246 DASWQN010000000
VERSION     DASWQN010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609490"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VGC82_04075"
     CDS             <1..493
                     /locus_tag="VGC82_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422482.1"
                     /translation="IRGNSRRRFDLKTEGTDMPFDEAAAIQFAAAHGVDGPAFQALPD
                     AAKYAWSWRAFNEPAYAALATQSNVHVVLYESLCARPEAFARRILAFAGLDWNRQTAN
                     FVARSTAHQGEAGYYAVFRNAVAAAEAWRRTMPQADQAATRRVVAASPLARFWPDLVD
                     PGA"
     gene            644..1267
                     /locus_tag="VGC82_04080"
     CDS             644..1267
                     /locus_tag="VGC82_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEY0422483.1"
                     /translation="MHDLVCPWCYLGVRRLLRTLHRRPDIVFELIWRPFLLNPDMPRM
                     GMSRPDYVVRKFGGEDRARRLYATISEVGRNEGLDFRFERIRRTPSSVDAHRLVRYAS
                     GYGVADSLVEALFAAHFTDGHDLGDHGVLVAVATACGMDPKAVYRFLASDAEAEGVHA
                     DNLRAHRLGINGVPCFVVDGAHAIAGAQEPEVIERMLDIATVDSARF"
     gene            1414..2139
                     /locus_tag="VGC82_04085"
     CDS             1414..2139
                     /locus_tag="VGC82_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0422484.1"
                     /translation="MTPEIKSELAPHGVLRAAINMSNFLLVTGRSASGDPEGVSPSTA
                     RAIADRLGVPVQYVPFAKPGELADAAGKDVWDIGLIGAEPARAEKIAFTAAYAEIEAT
                     YLVPPGSALKTIADVDKPGVRIAVTARSAYDLWLERNIKQATLIRSTSLDGAFEQFAA
                     EKLEALAGLRPRLLTDVQRMPGAVILPGQFTAVQQAVGTAKANAAAAAWLHEVVEEAK
                     SSGLVAKFIEQHKVVGLSVAPPG"
     gene            complement(2327..>2613)
                     /locus_tag="VGC82_04090"
     CDS             complement(2327..>2613)
                     /locus_tag="VGC82_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422485.1"
                     /translation="ETGPGAGGLLASTPSLARFIADHPVWNGSAAHLSGRELATRYGT
                     LDGTISGAVSRDDGLDFAFVFNRRTSDAEHDQITSLIHTVLNTHGAKLGS"
BASE COUNT          425 a          811 c          888 g          489 t
ORIGIN      
        1 gatacgcggc aacagccggc ggcggttcga cctgaagacc gaaggaaccg atatgccgtt
       61 cgatgaggcc gccgccattc agttcgccgc cgcccatggt gtggacggac cggctttcca
      121 ggcactgccg gacgcggcga aatatgcctg gagctggcgc gcgttcaacg agccggctta
      181 cgcggcactg gccactcaat caaatgtgca tgttgtattg tacgaatcgc tgtgcgctcg
      241 tccggaggca tttgctcgcc ggatcctggc gttcgccggg ctggattgga accgccagac
      301 cgcgaacttc gtcgcccgca gtactgctca ccagggggag gccggctact atgcggtatt
      361 ccgaaatgcc gtcgctgctg ccgaagcttg gcgccgcacc atgccgcaag cagaccaggc
      421 ggcaacgcgt cgtgtggtag ccgcctcgcc gcttgcccgt ttctggcctg atcttgtgga
      481 cccgggtgct tagcgggacg gttgatgtag ttccacgcag tcggatacgt ccccaccgcc
      541 ggcccttgga agacgatcgc ctcgtgacaa gtcgctggga aagcgtcaca cttccggggt
      601 gaatgttttc gcgcccctaa gggcacggct tcagatcgag gttgtgcatg accttgtttg
      661 cccctggtgc tacctggggg tccggcgttt gctgcggacg ttgcaccgcc gccccgatat
      721 cgtattcgag ctgatctggc gcccgttcct gctgaacccg gacatgcctc ggatgggcat
      781 gtctcgtccg gactatgtgg tgcggaagtt cggcggtgag gatcgggcgc gccggcttta
      841 cgcgacgatc agtgaagtcg gccggaacga gggcctggac tttcgtttcg agcgcatacg
      901 ccgaacgccg tcctcggtcg atgcccatcg tctcgtgcgt tacgcttcgg gctatggcgt
      961 cgcggatagt ctggtggagg cactgttcgc ggcccatttc accgatggac acgatcttgg
     1021 cgaccatggc gtgctggtcg cggtggccac agcctgcgga atggacccca aggcggtgta
     1081 tcgcttcctg gcgtccgacg ccgaggcgga aggcgtccat gccgacaatt tgcgcgcgca
     1141 ccggttgggg atcaacggcg tgccctgctt cgtggtcgat ggcgcgcacg caatcgccgg
     1201 tgcgcaggag cccgaggtga tcgaacggat gctggatatc gcgacggtgg attcggcgcg
     1261 cttctgatgc gccgcggcct tcttgtcttc gaactggtat ttcttttcgg cgtcgggtcc
     1321 tcttacggac aatcgccctg tcctggtccg ggttgcccaa gcccggttcc gccgcctacc
     1381 cttggtgtcg agatcaacaa gggaaacgcc ggcatgactc cagaaatcaa aagcgagctt
     1441 gcgccgcatg gggtgcttcg cgcagccatc aatatgtcca acttcctgct ggtcaccggc
     1501 cgcagcgcct cgggcgaccc ggaaggcgtt tcccccagca cggcacgtgc catcgcggat
     1561 cggctgggcg tgccggtgca atatgtgccg ttcgccaaac cgggtgagct ggcggatgcg
     1621 gcgggcaagg atgtgtggga catcgggctg atcggcgccg aacccgcgcg cgccgagaag
     1681 atcgccttca cagccgccta tgccgagatc gaggccacct atctggtgcc gccgggttca
     1741 gccctgaaga cgatcgccga cgtggacaag cccggcgtgc ggatcgcggt gaccgcccgg
     1801 agtgcctacg acctttggct ggaacgcaac atcaagcagg cgacgctgat ccggtccacc
     1861 agcctggacg gcgccttcga gcagttcgcc gccgagaagc tggaggccct ggccggcctg
     1921 cgtccccggc tgctgacgga tgtccagaga atgccgggtg cggtgattct gcccggtcag
     1981 tttaccgccg tgcagcaggc ggtcggaacg gcgaaggcca acgccgcggc tgcggcctgg
     2041 ctgcacgagg tcgtcgagga agcgaagtcg tccgggctgg tcgccaagtt catcgaacag
     2101 cacaaggtgg ttggtttgtc ggtggcacct ccgggctaga cgcgatcatg gcgactggaa
     2161 acagggttct ctcgcggctg caaaggaagt cgtgggtggc cgggccaggc aaggccatga
     2221 cgcatcggcg tcaggcggcg aactggccgg caccttttcc ggtcagcgtg aaaacgctct
     2281 ggagcgcgat cacgctggcg gaacggcgga ttcgccatgg gcggggtcac gaaccgagct
     2341 tggcaccgtg tgtgtttagc acggtgtgga tcagcgacgt gatttggtcg tgttcggcgt
     2401 cgctggtccg gcggttgaag acaaacgcga aatccagccc gtcgtcccga gacaccgccc
     2461 cgctgatcgt gccgtccagg gtgccgtagc gggtcgccag ttcccggccc gacaggtgcg
     2521 cagccgaacc gttccagacc ggatgatcgg cgataaaccg tgccaggctg ggcgtcgatg
     2581 ccagcaaccc gccggcgcct ggtccggtct cga
//
LOCUS       DASWQN010000247         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_158133, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000247 DASWQN010000000
VERSION     DASWQN010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158133"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1458)
                     /locus_tag="VGC82_04095"
     CDS             complement(277..1458)
                     /locus_tag="VGC82_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEY0422486.1"
                     /translation="MRTQVGIIGAGPAGLFLAHLLRRAGIGAINIESRARHDVEETVR
                     AGVLEHWVVELMQELGLGERMLQEGHFHDGITLQWNRERHHVDIQGLTDGKQVVVYPQ
                     HEVLKDLIAGRLADGGEIIFGVGGAAIRDIDTDHPAIDFHRDGAGRSDTIQCDYVVGA
                     DGFHGPGRQAIPPVHRTEYQKIYPFAWLGILTRAPLSWPELIYTNQDEGFALLSTRSP
                     DVQRMYIQCDPSDDIANWSDERIWDQLHARLGTDDGWSLTEGPIVQKGIIPLRSFVCE
                     PMQYGRLFIAGDAAHIVPPTGAKGLNLAVADVLVLSQALEAKYRHGRQELLDRYSATC
                     LRRVWKAERFSWYMTTMLHRNKHETDFEQRIHFADLDLVRTSTASARSLAENYVGLPF
                     D"
     gene            complement(1461..2342)
                     /locus_tag="VGC82_04100"
     CDS             complement(1461..2342)
                     /locus_tag="VGC82_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0422487.1"
                     /translation="MRAIDVHVHPMNPAYVQASLPFIAAAQRTFKGKFAARPDTQIAE
                     DFRRDDALAIPIAWDAESGAAGGIYSNEAVAALTRDYPDVFLPGWAMVDPWRGRKGLE
                     EIEHAIKTLGLLGVKYQPPVQGFSPNDRQFYPIWDLLQSLGAPVLIHCGTTAIGAGEP
                     GGLGFKLGHARPIPNIDDVAADFPRLNIVAAHPGWPWTEELIAVAIHKANVSIDVSGW
                     GPKYVPAPLKHDMQRRLQDKVLFGSDYPGWSPGQCCDEWEMEGFRPGVIEKLFHQNAT
                     RILNLQDAITRATAVRG"
BASE COUNT          445 a          767 c          836 g          477 t
ORIGIN      
        1 ttgcgggagg ttggctgacc ttgggtcagg ccagaagcga cgccaaggct tcgcccgcat
       61 gttgtcgtat aaactgatga cggagcccgt aatagtggtg gcggagtgcc taatcgtgat
      121 tgctggaacg tgcgtttagt gcgtttcaca atgactggaa acggtgttct cttgtggctg
      181 cgaaagaaat cgtggatggc cgtgcccagc acggccatcc acatgaacgt caggcggcaa
      241 ccaggctcgc cttttttcca accagcctga aacgctttag tcgaacggca gcccaacata
      301 gttttccgcc agcgatcgtg ctgaggccgt ggacgtgcga acaaggtcca ggtcggcgaa
      361 gtggatgcgt tgctcgaagt cggtctcgtg cttgttccgg tgcagcatcg tggtcatgta
      421 ccaggagaat cgttccgcct tccagacccg gcgcaggcag gtcgcgctgt agcggtccag
      481 cagctcctgg cgcccatgcc ggtatttcgc ctccagcgcc tgggacagca ccaacacgtc
      541 cgcgaccgcc aggttcaggc cctttgcgcc ggtcggcgga acgatgtggg cagcatcgcc
      601 ggcgatgaac aggcggccgt attgcatcgg ctcgcagaca aacgaccgca acgggatgat
      661 gcccttctgc acgatcggac cttcggtcaa cgaccagccg tcatcggtgc ccaggcgtgc
      721 atgcaactgg tcccaaatcc gctcgtcgga ccagttggcg atgtcgtcgc tggggtcgca
      781 ctgaatatac atgcgctgca cgtccggaga gcgcgtgctg agcaaagcga atccttcgtc
      841 ttgattggtg tatatcagtt caggccaaga taaaggtgcc ctggtcaaaa taccgagcca
      901 agcgaaggga tagattttct ggtattcggt gcggtggaca ggtgggatcg cctgccgccc
      961 cggcccatgg aaaccgtccg cgccgactac gtagtcgcac tggatcgtgt ccgatcgccc
     1021 ggccccgtcg cggtgaaagt cgatcgccgg atggtctgtg tcgatgtcgc ggattgccgc
     1081 accgccgacg ccgaagatga tttcgccgcc atcggccagc cggccggcga tcaggtcctt
     1141 caggacctcg tgctgcggat atacgacgac ttgcttgcca tcggtcagtc cctggatatc
     1201 gacgtgatgg cgctcacggt tccattgcag ggtgatgccg tcgtggaaat gcccctcctg
     1261 cagcatccgc tcgcccagcc cgagttcctg catcagttca acaacccagt gttccagcac
     1321 gccggcgcgg acggtttcct cgacatcgtg ccgggcgcgt gattcgatgt tgatcgcgcc
     1381 gatgccggcg cgccgcagca aatgggccag aaacaatccg gcgggaccgg cgccgatgat
     1441 tccgacctgg gtgcgcatgg tcagccccgg accgcggtgg cgcgggttat ggcatcctgc
     1501 aggttcagga tgcgcgtggc gttctggtgg aacagcttct cgatcacgcc gggacgaaaa
     1561 ccctccatct cccactcgtc gcagcattgg ccggggctcc agcccggata gtcggagccg
     1621 aacaacacct tgtcctgcag gcggcgctgc atgtcgtgct tgagtggggc ggggacgtat
     1681 ttcggtcccc agccagaaac atcgatcgac acgtttgcct tatggatggc gacggcgatc
     1741 agttcctcgg tccagggcca gccgggatgg gcagcgacga tgttcaggcg gggaaagtcc
     1801 gcggcgacgt cgtcgatgtt ggggatcggg cgggcgtgcc caagcttgaa ccccaggccg
     1861 ccaggctcgc cggcgccgat ggcggtggtg ccgcaatgga tcagcaccgg cgcgccgagg
     1921 gactgcagca ggtcccagat cgggtagaac tgcctgtcgt tcggcgaaaa tccctgcacc
     1981 ggcggttggt atttcacacc gagcaggccg agggtcttga tggcgtgctc gatctcctcc
     2041 aggcccttgc ggccgcgcca gggatcgacc atggcccaac ccggcaggaa gacgtcgggg
     2101 tagtcgcggg tcagtgcggc gaccgcttcg ttcgagtaga taccgccggc ggcgccgctc
     2161 tcggcatccc aggcaatggg gatcgcgagt gcgtcgtcgc ggcggaagtc ctcggcgatc
     2221 tgggtgtcgg gacgggccgc gaacttgcct ttgaaggttc gctgggcggc ggcgatgaag
     2281 ggcaggctgg cctgcacgta ggccgggttc atcggatgga cgtgcacatc gatggcgcgc
     2341 aagacggtgt ctccctcgac tttcccagtt tgatcgcatg attcaagtgg cttgaacact
     2401 tcactcgatg gcgcggcccg gtccagaccc cagccgatgc gccgggcggt cgcctgacgg
     2461 ctcgcaaccg ggcagaggtc gtctgccggg cggctccgac gaacgagaac cggcagctaa
     2521 cgatc
//
LOCUS       DASWQN010000248         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_290278, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000248 DASWQN010000000
VERSION     DASWQN010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290278"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VGC82_04105"
     CDS             complement(<1..206)
                     /locus_tag="VGC82_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422488.1"
                     /translation="MQQRRNRPTLRPGYAAFTVAAITTTLIALLACNSKPSSKLSAEK
                     TPNHYSSGQASDDPGINLNCVYDR"
     gene            complement(677..1495)
                     /locus_tag="VGC82_04110"
     CDS             complement(677..1495)
                     /locus_tag="VGC82_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0422489.1"
                     /translation="MPITLTQQTALITGASRGIGRGIALKLADQGVKRIAINYKENDA
                     AAEDAARLLKERGAVPLVIKADIGKVADIQRMFETIKSQFGGLDIFVSNARATLATGF
                     YAEPMEIPLEAWQATFDTQATEFLVAVRAAVPMMGKNGRIIAITYAPGGQTGSWQPWV
                     AMGSAKAAKEALARYFAVALAPKGITVNIISPGACDDSVLSGLPPQVFQMIKDWHESG
                     WTPMRRLGLPADVGNAVVLLCTDQASFITAQLLYVDGGASAMEPVFPLAIQGIK"
BASE COUNT          361 a          505 c          478 g          372 t
ORIGIN      
        1 aatcggtcgt aaacgcagtt gaggttaatc ccgggatcgt cgctggcctg acccgacgaa
       61 taatggttag gtgttttttc cgccgacaat ttggagctgg gtttggaatt gcacgccaac
      121 aacgcaatca gcgtggtggt aattgccgcc acggtgaacg ccgcataacc cggcctcaac
      181 gtcggacgat ttcttcgttg ctgcatagtt gtgttcctcg cgtacgaatg actgaaacat
      241 ttggccaagt taccgactgt tgttttgccg ccatgtcgcc aatgatggga atccgatact
      301 ccgctggcta tatgcctggc acatgacgaa cagccagaat aaaaacaggc ccagacagat
      361 cacgagtcgt actgcaattc tctgctcgtt ccctgacaag ttaggcgcgg acgccgaaat
      421 acttgccggg acctagcccc tcaatccgga acgaccgtcc gagaaggtgc ggaaggagca
      481 aattatccca cattcctttg atgtcaaatt gatagttgca gccacgacca cggcccgtga
      541 ggatcaacta ccgatcctcg aattccaccc agagaatttt gtaccccggc aaaaacacaa
      601 gatcgaactt ttggaatgtg tcgaactgga ttttaaaacc tgctgcctag aaatcaattc
      661 ccaacgcctc gattcgctac ttgatccctt ggatggccaa agggaagacc ggttccatcg
      721 cggaagcgcc gccatcaacg tacagcagct gggcggtgat gaagctcgcc tggtcagtgc
      781 agaggagaac cacggcgttg ccgacgtcgg ctggcaggcc gaggcgcctc atgggcgtcc
      841 agccgctctc gtgccagtcc ttgatcatct ggaacacctg gggcggcagc ccgctgagga
      901 cgctgtcgtc gcaggcgccc ggactgatga tgttcaccgt gatgcctttc ggggcgagag
      961 caaccgcgaa ataacgagcc agcgcctcct tcgcggcttt cgctgagccc atggcgaccc
     1021 aaggctgcca gctgccggtt tgcccgccgg gcgcataggt gatggcgatg atccggccgt
     1081 tcttgcccat catcggcacc gctgcgcgga ccgcaaccag gaactccgtc gcctgggtgt
     1141 caaacgttgc ctgccatgcc tccagcggga tctccattgg ttccgcgtag aagcctgtgg
     1201 cgagcgtagc cctggcgtta gaaacgaaga tgtccaggcc gccgaattgg gacttgatgg
     1261 tctcgaacat gcgctggatg tccgcaacct tgccgatatc cgccttgatc accagaggca
     1321 ccgcaccacg ctctttgagg aggcgagcgg cgtcttcggc ggccgcgtcg ttctccttgt
     1381 agttgattgc aattcttttc accccctggt cagcgagctt cagcgcgatt ccgcggccaa
     1441 taccgcgtga tgcccctgtg atgagagcgg tctgctgcgt gagcgtgatt ggcatgacac
     1501 cctccttttc gcatgtaaag gatcgacagg atcgatctcg atctcgtgta ctacactctg
     1561 catgcccgtg tggcccgaca gctaggattt aattcatcac ttgccagtca ggtagcgtcc
     1621 cgggtaagcg caaatcccga agggtcgact gccctggcta ctaataatga cgtgcaaacc
     1681 ggacggagtc tgcgcaagta tttcgctccg ctctcg
//
LOCUS       DASWQN010000249         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2269739, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000249 DASWQN010000000
VERSION     DASWQN010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2269739"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VGC82_04115"
     CDS             complement(<1..549)
                     /locus_tag="VGC82_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEY0422490.1"
                     /translation="MVYAPVRPDRALPPVRVNLYSDTQTLPSAAMKEAMMRAEVGDEQ
                     CGDDPTVNALCDQMAALTGKQAAMFLPTGTMCNQISILVHCRPGDEILAHEDSHIIIN
                     EGGGPGALAGAVILGLRGARGMFDAATLTAALRERRRNAPPQTLVAVEQTANAGGGSV
                     WPVALLNDVLGAAHDQDMKTHMD"
     gene            complement(602..1075)
                     /locus_tag="VGC82_04120"
     CDS             complement(602..1075)
                     /locus_tag="VGC82_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEY0422491.1"
                     /translation="MPTHAERKVVPYTPEQLFDLVADVERYPRFLPWCVAARVRSHIG
                     CELVADLTIGFGPFRESFTSRVTLERPKRVKVKYENGPFRYLNNQWDFAPRGTGTEVA
                     FFVDFEFRSRILQAAIGVVFNEAVRRMVNAFLKRAREVYGPPPVLQPPAPGSPAA"
     gene            complement(1075..2025)
                     /gene="lipA"
                     /locus_tag="VGC82_04125"
     CDS             complement(1075..2025)
                     /gene="lipA"
                     /locus_tag="VGC82_04125"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY0422492.1"
                     /translation="MDDRVNQPRHPEKANRPDNPIQRKPAWIRVKAPTHAVYHQTKAL
                     MRENGLVTVCEEAACPNIGECWSQRHATMMIMGDTCTRACSFCNVRTGQPVRLEADEP
                     ARIADAVAKLGLRHVVITSVDRDDLTDGGAGHFAAVIRAIRLATPATTIEVLTPDFLR
                     KPTGAETVAAARPDVFNHNLETVPRLYPTIRPGARYYQSLRLLDRVKQLDPSVFTKSG
                     LMVGLGETKAEIMQVMDDLRIADVDFLTIGQYLQPTVKHAAVDRFVPPEEFAEYASLA
                     RAKGFLLVSATPLTRSSYHADSDFAALRAARDVKLAQPAA"
BASE COUNT          419 a          709 c          686 g          401 t
ORIGIN      
        1 gtccatatga gtcttcatgt cctgatcatg ggcggcacct agcacgtcgt tcagcagcgc
       61 cacgggccaa accgacccgc cgccggcgtt ggcggtttgc tccaccgcca ccaaggtctg
      121 cggcggggca ttgcggcgcc gttcccgcag tgctgccgtt agcgtggcgg catcgaacat
      181 cccgcgtgcg ccgcgaagac cgagaatgac cgcaccagcc agggcaccag ggccgccgcc
      241 ttcgttgatg ataatatgcg aatcctcatg cgccaggatc tcgtcgcccg ggcggcaatg
      301 aaccaggatg gagatctggt tgcacatcgt cccggtcggc aggaacatcg ccgcctgctt
      361 gccggtcaac gccgccatct ggtcgcatag cgcgttcacc gtgggatcat cgccgcattg
      421 ttcgtccccg acctcggctc gcatcatcgc ttccttcatc gcggcggacg gaagcgtctg
      481 ggtgtcggaa taaagattga cccgcaccgg cgggagagcc cgatccggcc ggacaggtgc
      541 atagaccatg gaagcctcct ctgggattgc tgaccaagag gtcagtggac gtccgccggc
      601 atcaagcagc cggcgacccg ggcgctggcg gctgcaggac cggtggcgga ccatagactt
      661 cccgggcgcg cttgaggaag gcgttgacca tccgccgcac cgcctcgttg aagaccacgc
      721 cgatcgcggc ttgcaggatc cggctacgga attcgaaatc gacgaagaac gccacctcgg
      781 ttccggtgcc gcgcggcgcg aaatcccact ggttgttcag atagcgaaag ggtccgttct
      841 cgtacttcac cttaactcgt tttgggcgct ccagcgtgac gcggctggtg aaactctcgc
      901 ggaacgggcc gaagccgatg gtcaggtcgg cgaccaactc gcaaccgatg tggctgcgga
      961 ccctggcagc gacgcaccag ggcaggaagc gcgggtatct ttcgacgtcg gccaccagat
     1021 cgaacaactg ttccggtgtg tatgggacga ccttccgttc cgcatgcgtc ggcatcaggc
     1081 ggcaggctgc gccagtttta cgtcccgcgc agcccgcaac gccgcaaaat cggaatcggc
     1141 gtgataggaa ctacgggtca acggagtcgc cgagaccagc aagaatccct ttgcccgggc
     1201 gagcgaggca tactcggcaa attcctccgg cgggacgaag cgatccaccg ccgcatgctt
     1261 taccgtcggc tgcagatatt ggccgatggt caggaaatcg acatcggcga tgcgcagatc
     1321 gtccatgacc tgcatgattt ccgctttggt ttcaccaagc ccgaccatca ggcccgattt
     1381 ggtgaatacc gaaggatcaa gctgtttcac ccggtccagc agccttaatg actggtagta
     1441 gcgcgcgccg ggccggatcg ttggatacaa ccggggcacc gtctccaggt tgtggttgaa
     1501 tacgtcgggc cgagccgcgg cgacagtttc ggcaccggtt ggcttgcgga ggaaatcggg
     1561 tgtcaggacc tcgatcgtag tggccggagt cgccagccgg atcgcccgga tcaccgcggc
     1621 gaagtgccct gccccgccat ccgtcagatc gtcgcggtca accgaagtaa tcaccacatg
     1681 gcgcaaccca agcttcgcca ccgcatcggc gatgcgggcc ggctcatctg cttcaagccg
     1741 caccggttgg ccggtgcgca cgttgcagaa gctgcaggcc cgggtgcagg tatcacccat
     1801 gatcatcatg gtggcatgcc gctgagacca gcattcgccg atgttcgggc aagcggcctc
     1861 ctcgcacacc gtcacgagac cattttctcg catcagtgcc ttggtttggt gatacaccgc
     1921 atgggtcggc gccttgaccc tgatccaggc cggcttgcgc tggatcgggt tatccggtcg
     1981 atttgccttc tccggatgcc tcggctggtt gacgcgatcg tccatacgag acccatcggc
     2041 aggacgatga tcgatgatca gacggctagt gggcatgacc actgatgtgg acggcggcca
     2101 gcaggccgtc aagcaccagg caacattcgc caaccgatca acgctttcaa tttcaaccct
     2161 gctggcaggt caaaacgcca gcaagaccga tccctgcgaa gccgcgcatc ggccg
//
LOCUS       DASWQN010000250         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2401280, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000250 DASWQN010000000
VERSION     DASWQN010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2401280"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VGC82_04130"
     CDS             <1..471
                     /locus_tag="VGC82_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEY0422493.1"
                     /translation="QARMDGQLRRNFQGYTDDPADALIGFGASAIGQSVWGYVQNVAD
                     IKAWRERIEAGDLATVRGLALTEDDGLRGEIIERLMCDLLVDVPAVLRRRGFPADFLD
                     SALDTLRPLVADGLARVDHGVITVPEAMRALVRRVASAFDAYLDPAAGRHAVAV"
     gene            complement(522..944)
                     /locus_tag="VGC82_04135"
     CDS             complement(522..944)
                     /locus_tag="VGC82_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0422494.1"
                     /translation="MANRELREIIRRTNPVTLPLNATVQQACKLMRDRRVGAILVTEN
                     DGRLAGLFTGRDAVGRVVAEALNPATTTLGSVMTVQPDILKPNARAIDALRMMQDGGY
                     RHLPILDGTKIVGIVSHGDFTGLERARLDDETGFWEML"
     gene            complement(980..1450)
                     /locus_tag="VGC82_04140"
     CDS             complement(980..1450)
                     /locus_tag="VGC82_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0422495.1"
                     /translation="MAVTAADIMTSPAIFVSPETNMADIASLLASKHFSAVPVCHPDH
                     TLAGIVSESDILKPFRESVRAKRDWWLGVIAEGEELPQDFLDYIRRDTRTASDVMVRQ
                     VFTAEESTTLPELAELMIKHAIKRLPIMRDGKLIGVVSRADLVAAIAASPAMLV"
BASE COUNT          279 a          474 c          587 g          336 t
ORIGIN      
        1 caggcgcgga tggatggaca gctgcggcgg aacttccagg gctataccga tgatccggcc
       61 gatgcgctga tcggtttcgg tgcatcagct atcggtcagt cggtctgggg gtacgtgcag
      121 aatgtcgccg acatcaaggc atggcgggag cggatcgagg cgggtgatct ggcgactgtt
      181 cgggggctgg cgttgaccga ggatgatgga ttgcgcggcg agatcatcga gcggctgatg
      241 tgcgacctgc tggtggatgt gccggcggtg ctgcggcggc gcggatttcc ggcggacttc
      301 ctggacagtg cgctggatac gctccgtccg ctggtggctg acgggctggc gcgggtggac
      361 cacggcgtga tcacggtgcc ggaggcgatg cgggcgctgg tgcggcgggt ggcgagcgcg
      421 ttcgatgcgt atctggaccc ggcggcagga cggcacgccg tggcggtgtg acaaagcggt
      481 gtggcaagcc ggttcgcgtc gatgcgctgc cggatccctg atcagagcat ttcccagaag
      541 ccggtttcgt catcgagcct tgcccgctcc agcccggtga agtcgccgtg ggagacgatg
      601 ccgacgatct ttgtgccgtc gagaatcggc aggtggcggt agccgccatc ctgcatcatc
      661 cgcaaggcgt cgatggcccg ggcgttcggc ttcaagatat cgggctggac ggtcatgacc
      721 gaaccgagcg tggttgtggc tgggttgagt gcctcggcga caacgcgtcc gacggcgtct
      781 cgcccggtga acaatcctgc caggcgtccg tcattctcgg tcaccaggat ggcgccgaca
      841 cggcgatccc gcatcagctt gcaggcttgt tgcacggttg cgttcagcgg aagcgtgact
      901 ggatttgtcc gtcggatgat ttcgcgaagt tcccggttgg ccattctagg atccccctga
      961 tcgccggcgg ttcggcacgc tatacgagca tggcgggact tgctgcgata gcggccacaa
     1021 gatcggctcg gctgacgacg ccgatcagct tgccgtcgcg catgattggc aggcgcttga
     1081 tcgcgtgctt gatcataagt tccgctagtt ctggcagggt tgttgattct tcggccgtga
     1141 aaacctggcg gaccatgacg tcactcgcgg ttcgggtgtc ccggcgaata taatccagga
     1201 aatcctgcgg cagttcttcg ccttcagcga tcacgcccag ccaccagtca cgcttggcgc
     1261 gcaccgattc gcggaatggc ttcaggatgt cgctctcact gacgatcccg gcaagggtgt
     1321 gatccgggtg acataccggg acggcactga aatgcttgct ggccagcagc gatgcgatgt
     1381 cggccatgtt ggtttcgggc gaaacgaata tggccggcga cgtcatgatg tctgcagctg
     1441 ttacggccat tgggtatcct ccagtttaag cgcgcagaat gccatgaccg cgtgacatgt
     1501 cattgatcgg ggtcaaaccg gggctgcgcg ccaggtgccg gccatcgata aagaccaata
     1561 cgtcaccctg atgtgaaggg cccgaagcac gcggccaaga ccggtcgcgg tgcgactgac
     1621 gcgtcgttgc gatcggctgc taagccccga ccatcaggcg gacgatctcg tcgctg
//
LOCUS       DASWQN010000251         2834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1741817, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000251 DASWQN010000000
VERSION     DASWQN010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741817"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VGC82_04145"
     CDS             <1..1299
                     /locus_tag="VGC82_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase family protein"
                     /protein_id="HEY0422496.1"
                     /translation="RLENRWQAWLDVEVALARAQAELDIIPAAAAEAIAEAARLENLD
                     RARIDEGFARTGHTIVPLVWELSRVTGEQHGGWVHWGATTQNITQTGDLLVLRQAHGI
                     FLRQIGETLSAMAGLAERGAEMPIAGRTHGQHAVPATFGYKVAVWIDELIRHTERFRQ
                     AAPRLFVAMLGGGAGTYASLGKNGPPVQQAIGRQLGFGSMTVPSRAIGDHLAENILLL
                     GLLAATCGKIGREIYTLMKTEFGEVEEPVPPGTVGSSTMPQKRNPKLCQDIIAAAAEI
                     RAMVPLALEAMTTEHEADRTTSLMMDTAESRACIATGDMLSRLAEIMRGLRLDPARMR
                     SNLDLGGGLIMAEAVMLDLGMALGRQHAHDAVYDAAQEAFLQGRPFSELLAADARVAA
                     RMTPEQIKGLLDPTAYTGLCAEMAREGATRARAAATELLQ"
     gene            1296..2144
                     /locus_tag="VGC82_04150"
     CDS             1296..2144
                     /locus_tag="VGC82_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0422497.1"
                     /translation="MTQLDVDHLRSWIGRQETLHDQVTRFPAAALSATLDRDDPPPQA
                     GDPLPPLWHWLYFLPTAAQSALGPDGHPARGGFLPPVPLPRRMWAGGRFVFHQELLMD
                     EAITRQSTVQDVTVKQGRNGALCFVLVRHEISGRNGLAVVEEHDIVYRDMPLPHETPA
                     EPRPARNDGVWQRDIHPSESMLFRYSALTFNSHRIHYDRRYVTETEGYPGLIVHGPLI
                     ATLLIDLLRRNTSRPVATFRFRAVSPLFDIAPFSVHGAPDNEGGAALWARNVGGHLAM
                     EAEATF"
     gene            complement(2538..>2834)
                     /locus_tag="VGC82_04155"
     CDS             complement(2538..>2834)
                     /locus_tag="VGC82_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422498.1"
                     /translation="YNSPQWTTQSSNVLGASVAPEGLLFAERRNRGYTLYSVQSGALV
                     ARLRPTGSDDSFEIFYWSLWKNRWASTGRLAAPSCRSVTPQRFITYGDIFWAMT"
BASE COUNT          512 a          979 c          866 g          477 t
ORIGIN      
        1 cgcctggaaa accgctggca ggcgtggctg gacgtcgagg ttgcgctcgc ccgagcccag
       61 gctgaactcg atatcattcc cgccgctgcc gccgaggcca tcgccgaggc tgcccgcctg
      121 gagaacctgg accgggcccg catcgacgaa ggcttcgccc gcaccggcca caccatcgtt
      181 ccgctggtct gggaactcag ccgcgtcacc ggggaacagc atggcggctg ggtccactgg
      241 ggcgccacca cgcagaacat tacccagacc ggcgacctgc tggtcctgcg ccaggcgcac
      301 ggcatcttcc tgcgccagat cggcgaaacc ttgtccgcca tggcagggct cgccgaacgc
      361 ggcgccgaga tgccgatcgc cgggcgcacc cacggccagc acgccgttcc cgccaccttc
      421 ggctacaagg tcgccgtgtg gatcgacgag ctgatccggc acaccgaacg cttccgtcag
      481 gccgcaccgc gcctgttcgt cgccatgctg ggtggcgggg cagggaccta tgcctccctc
      541 ggcaaaaacg gtccgccggt gcagcaagcc atcggccgcc agctcggttt tggctccatg
      601 acggtgccgt cgcgcgccat cggcgaccac ctggcggaaa acatcctgct gctgggcctg
      661 ctcgcagcca cctgcggcaa gatcgggcgg gaaatctaca cgctgatgaa gaccgagttc
      721 ggtgaggtcg aggaacccgt cccgcccggc accgttggca gttccaccat gccgcagaag
      781 cgcaacccga aactctgcca ggacatcatc gccgccgccg ccgagatccg cgccatggtg
      841 ccgctggccc tggaagcgat gacgaccgaa cacgaggctg accgcaccac cagcctgatg
      901 atggacaccg ccgaatcccg cgcctgcatc gccaccggcg acatgctgag ccgcctcgcc
      961 gagatcatgc gcggcctgcg cctggacccg gcgcgtatgc gcagcaacct cgacctcggc
     1021 ggtggtctga tcatggccga ggcggtgatg ctggaccttg gcatggccct cggccgccag
     1081 cacgcgcacg acgcggtcta tgacgcggcc caggaggcat tcctgcaggg ccggccgttc
     1141 agcgagctat tggccgcgga tgcgcgggtt gccgcccgca tgacgccgga gcagatcaag
     1201 ggcctgctcg atcccaccgc ctacaccggg ctatgcgccg agatggcccg cgaaggcgcc
     1261 accagggcgc gggctgccgc gacggagttg ttgcaatgac tcaactggat gtggaccatt
     1321 tgcggtcctg gatcggcagg caggaaacgc tgcacgatca ggtcacgcgc ttcccggctg
     1381 ctgcgctgtc cgcgacactg gatcgcgacg acccgccacc ccaggcgggc gatccgttgc
     1441 cgcccttgtg gcactggctt tatttcctgc ccaccgctgc gcagtccgcg ctgggaccgg
     1501 acggccaccc ggcgagggga ggcttcctgc ccccggtccc gctgccccgg cgcatgtggg
     1561 ccggcgggcg gttcgtgttc caccaggagc tgcttatgga tgaggcgatc acccgccaat
     1621 ccaccgtcca ggacgtgacg gtcaaacaag gccgcaacgg cgcgttgtgc ttcgtcctgg
     1681 tacgccatga gatttccggt cgcaacggcc tggccgtggt ggaggagcac gacatcgtct
     1741 atcgcgacat gccgctaccg cacgagactc cagccgaacc gcgtcctgcg cgcaacgacg
     1801 gcgtctggca gcgcgacatt caccccagcg aatcgatgct gttccgttac tcggcactaa
     1861 ccttcaacag ccaccgcatc cactacgatc gccgctacgt caccgagacg gagggctatc
     1921 ccggcctgat cgtccacggc ccgctgatcg ccacgctgct gatcgacctg ctgcgccgga
     1981 acacttcgcg cccggttgcc acgttccgct tccgcgcggt cagtccgttg ttcgatattg
     2041 ctccgttctc ggtacatggc gcaccggaca acgagggcgg tgccgcattg tgggcacgca
     2101 acgtcggcgg acatcttgcg atggaggcgg aggcgacgtt ttgatccagt cacggtgaaa
     2161 ccgctcttag ccaatctttg ccgctgaatt tcccgatcga taaccgggtg ccattgctcg
     2221 catccggaaa ccggaaccgt gcctgtgccg ctcatgcacg catagtatag ttgcagacat
     2281 tccttcgaac tggcagcatc cattgctggt ttcttatgcc gcgaccgacc gaacgccccg
     2341 cgagcgccgc cggttgagac tgctccactg ccggaaggcc aacagccgaa tgggcaatcg
     2401 aatcattcca atctgacgta atgatcgcgc catccggaat tctgactcaa cgtcggaaaa
     2461 cgttcctgcc atgaccagcc ggcacttatc tacgtggccc taagctcgac tttccgcatt
     2521 tttcatccgg ctggccgtca ggtcatagcc cagaagatat ccccgtaggt gatgaagcgt
     2581 tggggcgtca ccgatcggca agacggtgcg gccaaacggc ctgtcgaagc ccatcggttc
     2641 ttccagagcg accaatagaa aatttcgaag ctgtcgtcag atccagtcgg acgcaagcgt
     2701 gcgaccaacg cgccggactg gacactgtaa agcgtataac cacggttgcg gcgctccgcg
     2761 aacaaaagtc cctctggagc aacggaagca cccaatacgt tggatgattg ggtcgtccat
     2821 tgcggcgaat tgta
//
LOCUS       DASWQN010000252         1948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_949910, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000252 DASWQN010000000
VERSION     DASWQN010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949910"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VGC82_04160"
     CDS             complement(<1..624)
                     /locus_tag="VGC82_04160"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY0422499.1"
                     /translation="MSHDALQQPIEDLWEQRETLSSSTKGAARDAVEAALDALDSGQA
                     RVAEKGASGWQVNQWLKKAVLMSFRLTDSTPMEGPGGAPVWDKVPMKFAGWGENRFKE
                     AGFRAVPGAVVRRSAFIAPGAVLMPSFVNVGARVGRNTMVDTWATVGSCAQIGENCHL
                     SGGVGIGGVLEPLQANPVIIEDDCFVGARSEVAEGVIVGRGSVLSMGV"
     gene            complement(1023..>1948)
                     /locus_tag="VGC82_04165"
     CDS             complement(1023..>1948)
                     /locus_tag="VGC82_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEY0422500.1"
                     /translation="SCGVARQYTGSAGKITNCQIGVFAAYVSRHGHAFIDRALYLPKA
                     WTADAARLAAAHVPSDVGFATKPQLGVRMIERAIAAGVPFAWVAADTVYGVGAVETAL
                     RKAGKGYVLGVSSDHQFHSWDRKPPISGTAEDIARGLKSSAWRRLSAGDGTKGPRLYD
                     WTYVELADLDAAEFGAPHGGLWTRGLLIRRGIDDGEMAYFSTWCPAGTGIETLVAVEG
                     HRWAVEEGFEVAKTELGLDHNETRSWHGWHRHVSLVMLALAMLAMIRHRANSGREKNQ
                     PPRLRRQIHLPGALVGAGSSPHRRAAGAAAH"
BASE COUNT          359 a          722 c          547 g          320 t
ORIGIN      
        1 tacccccatc gacagcacgg agccccggcc aacgatcact ccttcagcca cttcagagcg
       61 cgcaccaacg aagcagtcgt cctcgatgat caccggattg gcctgcaacg gctccagcac
      121 accgccaatc cccacgccgc ccgacaggtg gcaattctcg ccgatctgcg cgcagcttcc
      181 caccgtcgcc cacgtgtcga ccatcgtgtt acgcccaacc cgtgcgccca cattgacaaa
      241 gctgggcatt agtacggctc ccggcgcgat aaacgctgac cgtcgtacca ccgcacccgg
      301 caccgctcgg aatccggcct ccttgaaccg gttctccccc cagccggcga acttcatcgg
      361 caccttgtcc cacaccggcg caccgccagg accctccatg ggggtcgaat cggtcaaccg
      421 gaacgacatc agcaccgcct tcttcagcca ctgattgacc tgccagcccg aggcgccctt
      481 ctcggccacc cgcgcctgcc cgctgtccaa ggcatcaagt gcggcctcaa ccgcatcgcg
      541 cgccgcgccc ttggtcgaag aagacagtgt ctcccgctgt tcccaaagat cctctatcgg
      601 ctgttgcagg gcatcatgag acatcgtgtg gccgtttcct ggtttgaagc ggccggacga
      661 tgcggtcggc ctccgatcct gtcaacgcgc tacgcagcgt ctgcctcagc gataataccc
      721 aggcgacccg taataaaccg gcggcggcgc ataatacccc ggtgccgcca tgtagggcgg
      781 ggcatagttc cgttctcgcc aatgacgctc ccgccactca tgctcgcgcc attcgtgttc
      841 ccgccgcaca tggtcacgcc actcatgccg ctcatgccga cgccagcctt cgtcagcgaa
      901 agccggtgca gcggcagcca ccgcgaacga gctacagttg cagtcttcgt ttccgatgcg
      961 ctcgttgtgc ggcggcctga tgagcgcggc gccacagcga ccaggtcatg accagcgccg
     1021 gctcaatgcg ccgctgcgcc agccgcacgg cgatgcggcg aacttcctgc accgaccaac
     1081 gcaccaggga ggtggatttg cctgcgtaac cttggcggct gatttttttc gcgcccgctg
     1141 ttggcgcggt ggcggatcat cgccagcatg gccaaggcca gcattaccag cgagacatga
     1201 cgatgccagc catgccagga cctggtttcg ttatggtcca gcccaagctc cgtcttggcc
     1261 acctcaaagc cctcttcgac cgcccagcga tgaccctcca ccgcgaccag cgtctcaatg
     1321 ccggtgccgg ccgggcacca ggtcgagaag tacgccatct cgccatcatc gatgccgcgc
     1381 cggatcagca ggccgcgggt ccacaggccg ccatgcggcg caccaaactc ggcggcatcg
     1441 agatcggcca gttcgacata cgtccagtca tacagccgcg gtcctttggt gccgtcgccg
     1501 gccgaaaggc gccgccaggc cgacgacttc agcccgcgcg caatgtcttc agccgtgccg
     1561 ctgatcggcg gcttgcggtc ccatgagtga aactgatggt ctgagctcac ccccagaacg
     1621 tagcccttgc cagccttgcg cagcgccgtc tccaccgcgc cgacgccgta gacggtatca
     1681 gccgccaccc aggcaaacgg cacgccggcg gcaatggcac gctcgatcat ccgtacccca
     1741 agctgcggct tggtggcaaa gcccacatca gagggcacat gcgccgctgc cagccgcgcg
     1801 gcatcggctg tccatgcctt cggcagatac agcgcccggt cgatgaaggc atggccgtgg
     1861 cgcgagacat aggcggcaaa cacgccgatc tggcagttgg taatcttacc cgccgaacca
     1921 gtatactgac gcgccacgcc gcaagagg
//
LOCUS       DASWQN010000253         4850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1610123, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000253 DASWQN010000000
VERSION     DASWQN010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610123"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VGC82_04170"
     CDS             <1..354
                     /locus_tag="VGC82_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422501.1"
                     /translation="GCLRTAMAATNLPLDDVLRLQDHAIDLDRSSQKVTKVLEARRKE
                     RARNPAAMTLENTQWDEGAFQLAINHSLEKLADANSKLAAYTATLAPAEPKPKLPFLL
                     AEQMTPSVLARRARN"
     gene            complement(410..1366)
                     /locus_tag="VGC82_04175"
                     /pseudo
     CDS             complement(410..1366)
                     /locus_tag="VGC82_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513520.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
     gene            complement(1430..2758)
                     /locus_tag="VGC82_04180"
     CDS             complement(1430..2758)
                     /locus_tag="VGC82_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0422502.1"
                     /translation="MSRVLHRSGGIPPVAVHGEGIYLHTADGRSIIDASGGAAVACLG
                     HGNRRVAEAVGRQAAAMAYAHTGTFSNQPAEELAEFLLHDEPGGLTRAWFCSSGSEGN
                     EAAIKLARQYFLEIGQPRRTRTIARRQSYHGTTLGALAAGGNMMRRAHYEPILSQAHS
                     LVSPCFAYRFKMDAETDAQYLDRLIDELDAEFQRLGPDTVMAFLAEPVVGATTGCVTA
                     LPGYFPRVRAICDRYGALLILDEVMCGMGRTGTMHAWQQEGISPDIQVVAKGLGGGYQ
                     PIGGILIGDRIVRALADGSGGFLHGQTYQAHPVACAAALEVQRIIGDDDLIANVQATG
                     SRLETALQERFGNHRHVGDIRGRGLFWALEFVTDRASKAVFDPALKLNERIKAEGMAR
                     GLATYPMGGTIDGKQGDHVVLAPPYISTAADIDTIVERLGEAVDAALNSL"
     gene            2899..4623
                     /gene="ilvD"
                     /locus_tag="VGC82_04185"
     CDS             2899..4623
                     /gene="ilvD"
                     /locus_tag="VGC82_04185"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY0422503.1"
                     /translation="MPTRSWDKSRMPSRHVTVGPSRAPHRSYYYAMGLTEEEIDQPFV
                     GVATCWNEAAPCNIALSRQAQSVKKGVSETGGTPREFTTITVTDGIAMGHQGMKSSLV
                     SRDLIADSVELTVRGHCYDALVGLAGCDKSLPGMMMAMLRLNVPSVFMYGGSILPGKF
                     KGRDVTVVDVFEAVGQHAAGGMSDEELHELECVACPSAGACGGQFTANTMATVSEAIG
                     LALPGSAGSPAPYEERDRFAVESGRAVMRLIEQRIRPRDIVTLKALQNAAVVVGATGG
                     STNAGLHLPALAHEAGIRFTMDDVVEIMRRTPYIADLKPGGKYVAFDVHNVGGIPVII
                     KALLDGGLLHGDCLTVTGKTLAENHKDVIFPTGQDVIYPVSNPISPTGGVVGLKGSLA
                     PDGAIVKVAGLHNLRFEGTALCFDSEEEAYAAVDARAYKEGDVIIIRYEGPKGGPGMR
                     EMLSTTAAIYGQGMGEKVALVTDGRFSGATRGFCIGHLGPEAAVGGPLALVNNGDKII
                     IDADKGSIDLIVPDAELAARKAAWKPRRTDYNAGAIWKYAQLVGPAHLGATTHPGTEA
                     ETHCYADI"
BASE COUNT          899 a         1504 c         1596 g          851 t
ORIGIN      
        1 ggttgcctgc gaaccgcgat ggcagccacg aacctgccgc tcgacgatgt gctgcggctc
       61 caggatcatg cgatcgacct cgaccgctcc tcgcaaaagg tgaccaaggt gctggaggct
      121 cggcggaagg aaagggcacg gaaccccgcc gcgatgaccc tggaaaacac gcagtgggac
      181 gaaggtgcct tccaactggc gatcaatcat tcgctagaga aactggcgga tgccaatagc
      241 aagctggcgg cctatacggc cacgctggct ccggccgaac cgaagccgaa gttgccgttc
      301 ctgttagccg aacagatgac gccctccgtc ctggcccgcc gagcgcgaaa ctagatcacg
      361 ccgggcggaa cgttcgaagc gtgtgaccgg tccgatctga gggtggcggt caactgctgc
      421 cgatcaatat gccgaccccg aacaccagtg ctccgccaaa ggcaacctga accgccgccg
      481 ataatggcgg agtgtccatg tatttccaac gtatccacga gatcaccgcc aattccacga
      541 tgaccacggc gattgccacc gccacggcgg tccagaacga cgggatcagg aacggcaagg
      601 tatgaccgat gccgccggcc gtcgtcatca ggccgctgat cccgccgcgg atcaacggtg
      661 cgccccgtcc ggtcaggctg ccgttgtcgg acaacgcttc ggcgaaaccc atggaaattc
      721 ccgcgccgac cgatgcggcc atgccgacca ggaacgtttc ccaggtattg ccggaagcaa
      781 aagcagccgc gaaaactggc gccaatgtcg atactgaacc gtccatcagc ccggccagac
      841 cgggctggat gatccgcagc acaaaggatt ggtgggcgtg ggcgtcctct ttccgccgca
      901 catccgccgg caggttttcc tcgaccaatt gatcggcgcg gtgttcatgc tcgacttccg
      961 cgtcggcaag atcgccgagg agtttgcgga tggtgacatc ctcgctgcgc gtggcggcac
     1021 ggcggtagaa actctgggtt tccgtctcca tgctttccgc caggttgcga accgcgttca
     1081 ggttcagcgg taccatttgc cagaccggct tatgctggac gaatccgcgc acgtcctgac
     1141 gccggatcag ggggatgtgt tcgccaaatt taccgcggta aacgtcgagc agcctacggc
     1201 ggtggtcgcc ttcctctcct gccatttcgg taaacacggc ggcgctgccg ggatactacg
     1261 aacggagccc ttcggcgatg tccaggtaga tgcggccgtc ctcctcctcg ttggcgatcg
     1321 cgagggcgag gatctcccgt tcagtcagtt ctgagaaatt tcgcatggcg cggaatgtgg
     1381 ttgagttggc ggtgccgtca agtggggagc gtaatgatgc gaatgcttct cagaggctgt
     1441 tgagagcggc atccacggct tcgcctagcc gttcgacgat cgtgtcgata tcggctgcag
     1501 tggaaatgta gggtggggcc aggacgacgt ggtcgccttg tttgccgtcg attgtgccgc
     1561 ccattggata ggtggcaagg ccacgggcca tcccctccgc cttgatccgt tcattcagtt
     1621 tcagcgcggg atcgaagact gctttggaag cacggtccgt gacaaattcc aacgcccaga
     1681 acaggccgcg gccgcggatg tcgccaacgt gccggtggtt gccgaaccgt tcctgcaacg
     1741 cggtttccag ccggctgccc gtcgcctgga cattggcgat caggtcgtcg tcgccaatga
     1801 tccgctggac ctccagcgcg gcggcgcagg caacgggatg cgcctgatac gtttgcccgt
     1861 gcaggaatcc gccagaacca tccgcaagcg cccggacgat gcggtcacca atcaggatcc
     1921 cgccgatcgg ctggtagccg ccgccgaggc ccttggcaac gacctggatg tcgggggaaa
     1981 ttccttcttg ctgccacgcg tgcatcgtgc cggtgcggcc catgccgcac atcacctcgt
     2041 ccaggatcag cagcgcgcca tagcggtcgc aaatcgcgcg gacgcgtggg aagtagccgg
     2101 gcagggcagt cacgcagccg gtggtggcgc cgacaaccgg ttcggccaga aacgccatca
     2161 ccgtgtccgg gccaaggcgc tggaactccg catccagttc gtcgatcaac cggtccaggt
     2221 attgtgcgtc ggtttccgca tccatcttga agcgataggc gaagcacgga gacaccaggc
     2281 tgtgcgcctg gctgaggatc ggctcgtagt gtgcccggcg catcatgttg ccgccggccg
     2341 ccagggcccc aagcgtagtg ccgtgatacg attggcggcg tgcgattgtg cgcgtgcgcc
     2401 ggggctggcc aatttccagg aaatattggc gagccagctt gatcgcggct tcgttgcctt
     2461 ccgaaccaga ggagcagaac caggcgcggg tcagcccgcc gggttcatca tggagcagga
     2521 actctgccag ctcctcggcc ggctggttgg aaaacgttcc agtatgggca taggccatgg
     2581 cggcggcctg acgccccacg gcttcggcga cgcggcggtt gccgtgaccg aggcaagcga
     2641 ccgcggctcc acccgatgcg tcgatgatcg aacgtccgtc ggcggtatgc aggtagatac
     2701 cttcgccatg gacggcgacg gggggaatgc cgccagaacg atgcagcacg cggctcattt
     2761 tttcgggtct ccgatcacgg tgagcatggc gcagcgacgg ccatgcggca aggtgagcaa
     2821 aacccgccca tcgtgccttt gccgcctttg gttccgcggc gctataagcg cgataccgac
     2881 actcagaagg aagcgatcat gccgaccaga agctgggata aatccaggat gccatctcgc
     2941 catgtcacgg tggggcccag ccgagcaccg catcgcagtt actattacgc gatggggctg
     3001 accgaggagg agatcgacca gccttttgtc ggcgtcgcca cgtgctggaa cgaagccgcc
     3061 ccgtgcaaca tcgcgctaag ccgtcaggcg cagtcggtga agaagggagt ctccgaaacc
     3121 ggtggcacac cgcgcgaatt caccaccatc acggtgaccg acggcatcgc catgggccac
     3181 caggggatga agtcgtcgct ggtctctcgc gaccttatcg ccgacagcgt cgaactgacg
     3241 gtgcggggcc attgctacga tgccctggtc ggactggctg ggtgcgacaa gtcgctgccg
     3301 ggcatgatga tggcgatgct gcggctaaat gtgccgagcg tgttcatgta cggcggttcc
     3361 attctgccgg ggaagttcaa gggccgcgat gtcaccgtgg ttgacgtgtt cgaggcggtc
     3421 ggccagcacg cggccggcgg catgtcggat gaagagctgc acgaactgga atgtgtggcg
     3481 tgtccctcgg ccggggcctg tggcggccag tttacggcca acaccatggc gacggtgtcc
     3541 gaggcgattg gcctcgcgct gccgggatct gccggttctc cggcgccgta tgaggaacgc
     3601 gaccggttcg cggtggagtc cggccgcgcc gtgatgaggc ttatcgagca gcgcattcgc
     3661 ccgcgcgaca tcgttacgtt gaaagcgttg caaaacgccg cggtggtggt tggcgcgacc
     3721 ggcggctcga ccaatgccgg gctgcacctg ccggcgctgg cgcacgaggc gggcatccgc
     3781 ttcaccatgg acgacgtggt agagatcatg cgacgcacgc cctacatcgc cgacctcaag
     3841 ccgggcggca aatacgtcgc cttcgacgtc cacaatgttg gtggcatccc ggtcatcatc
     3901 aaggcactgc tggatggcgg cctgctgcac ggcgactgcc tgacggtgac cggcaaaacg
     3961 ctggcggaga accataagga cgtcattttc ccgaccggcc aggacgtcat ataccctgtg
     4021 tcgaacccga tttccccaac cggcggcgtt gtagggctaa agggcagcct ggcgccggac
     4081 ggcgcgatcg tgaaggtcgc cgggctgcac aatcttcggt tcgagggcac cgccctgtgt
     4141 ttcgactccg aggaggaagc ctacgccgcg gtggacgccc gtgcctacaa agaaggcgac
     4201 gtgatcatca tccgttatga gggtccgaaa ggtggtccgg gcatgcggga gatgctgtcc
     4261 accacggcgg ccatctatgg ccaggggatg ggcgaaaaag tcgcgctggt caccgacggc
     4321 cggttttcgg gtgccacgcg cgggttctgt atcggccacc ttgggccgga ggcggcggtc
     4381 ggcgggcccc tggcgctggt gaacaacggc gacaagatca tcatcgacgc cgacaagggc
     4441 agcatcgacc tgatcgtgcc ggatgcggag cttgcggcgc gcaaggcggc ctggaagcct
     4501 cggcgcaccg actacaacgc cggggcgatc tggaaatacg cgcaactggt gggaccggcg
     4561 catctggggg cgaccaccca tccgggaacc gaggcggaga cccattgtta cgccgacatc
     4621 tgaagacgct gctgttacgt tccggcaccc accgttacag gtgctggaag tagactggga
     4681 taagcacctc gttgaggagt tgaccgatga agtggcttgc catcggcgcg gccgtcttgc
     4741 tgatcggcac ggccagcggc agcgcccaga cgccaccgcc attcgcgccg attcctccgc
     4801 cgcgacagga attcgttccg cctcatcccg gtgggcgcct tgtatggcag
//
LOCUS       DASWQN010000254         3805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_949977, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000254 DASWQN010000000
VERSION     DASWQN010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949977"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..987)
                     /gene="cysK"
                     /locus_tag="VGC82_04190"
     CDS             complement(10..987)
                     /gene="cysK"
                     /locus_tag="VGC82_04190"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY0422504.1"
                     /translation="MASEHKPLVFDRPLRGRIYDSIAETIGNTPLVRIPKILAEEGIT
                     ADVVLKLEFFNPISSVKDRIGVSMIVELEASGRLKPGGTLIEPTSGNTGIGLAFVAAA
                     RGYRLILTMPESVSMERRKMLAYLGAELELTPREKGMNGAIARAKELLEATPGAVIPN
                     QFGNPANPAIHIKTTAEEIWYDTAGEVDAIISGIGTGGTLTGLAQALKPRRPGLKMFA
                     VEPEASAVLSGGKPGPHPIQGIGAGFIPEVLNTEAIDEVIQVSNEETLAASRRLARTE
                     GIPGGISSGAALAAALKVAKRPEYSHKRIVVIIPSFAERYITTVLFEGL"
     gene            1209..2567
                     /locus_tag="VGC82_04195"
     CDS             1209..2567
                     /locus_tag="VGC82_04195"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEY0422505.1"
                     /translation="MNTFRTVPLLVLLLAAATTGTRADPARTADSARIGEAIRAGKAA
                     FGDWRGDAPGVVRLITPDSLPAPFAGRSAGQSPSVVGRPAGAAPHVPPGFEATLFATG
                     LEQPRTLRTAPNGDILVAEAGSGRIRVLRASDGTAKPVQAVTFASDLNLPFGLAFWPP
                     GPSPQFLYVGETGRIVRYPYQAGDLQARGAAQTIVASLPEGGHWTRDIVFSPDGGRMF
                     VAVGSEGNLAGDLTGQPPAGLPLGAAWGDNLNRAAVLEFSPEGGNQRIFATGLRNCSA
                     EAMQPGTGALWCAVNERDGLGDNLPPDYATHVEPNAFYGWPWFYIGNHPEPRMNGAHR
                     DIAGRVTVPDVLIQPHSAPLGIVFYDGAQFPPAYRGDAFVALHGSWNRAKRTGYKVVR
                     LPLQNGRPNGTYEDFLTGFVASDQAVWGRPVGLTVARDGSLLVSEDANGTIWRVTYRH
                     TP"
     gene            2564..>3805
                     /locus_tag="VGC82_04200"
     CDS             2564..>3805
                     /locus_tag="VGC82_04200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0422506.1"
                     /translation="MIGHSASVRFILVTLVIDSLGFGLVVPIVPSLVLQLSGLTVSAA
                     SMWVGALLTAFALMQFACAPVLGALSDRFGRRPVLLLSLGGICANYLLLAWAPSLAWL
                     FLGRLIGGATAANAATATAYIADVIPPGKRAGQFGLVGAMFGIGFIAGPALGGVLGAY
                     GLRLPFVAASILAGCNVLYGLFVLPESLPPARRRKIDWGRANPLGSLRVLMANRDLGR
                     LAIAWGSIWFALGALQTTFVLANEVRFGWDSRHNGLALALAGLGSALVQGLLVRRVVP
                     WLGERRAAMIGGMITAVAYLCIALAPVGWVVLLGIVLQSVGAITSPAVQGMVSALAAA
                     DRQGETQGALASVQGLTAIASPLVASWVFSTFTAVATPIFLPGAPFVVSALAFGLAIW
                     AVSGVRLAPALVGTGAGVVVSA"
BASE COUNT          545 a         1234 c         1271 g          755 t
ORIGIN      
        1 ccgctggtat cacagtcctt caaacaatac ggtggtgatg taccgttccg cgaacgacgg
       61 gatgatgacg acgatccgct tgtggctgta ctctgggcgc ttggccacct ttaaagccgc
      121 cgccaacgcc gcaccggagg atatgccgcc gggaattccc tcggtccgcg caagccgcct
      181 ggaggcagcg agcgtttctt cgttggaaac ctggatgacc tcgtcgatgg cctcggtatt
      241 caggacttcg ggaatgaagc cggcaccgat gccctgaatc ggatgcggtc cgggcttgcc
      301 gccggacagc accgcgctcg cctccggctc cacggcgaac atcttcagcc ccgggcgccg
      361 cggcttcagc gcctgcgcca gtccggtcaa cgtgccgccg gtgccgatgc cggagatgat
      421 cgcatcgacc tcgcccgcgg tgtcgtacca gatttcctcc gccgttgttt ttatgtgaat
      481 cgccggattt gccggattgc cgaactgatt cgggatcact gcccccggcg tcgcctccag
      541 aagctccttt gctcgggcga tagcgccgtt catgcccttt tcccgcggcg tcagttccag
      601 ctccgccccc aggtaggcca gcatcttgcg gcgctccatc gagacggatt cgggcatcgt
      661 caggatcaac ctgtagccgc gcgccgcagc gacaaacgca aggccgatgc cggtgttgcc
      721 cgacgtcggc tcgattagcg tgccccccgg cttcagccgc ccagatgctt ccagttcgac
      781 aatcatgctg acgccgatgc ggtccttgac gctggagatc ggattgaaga actccagctt
      841 caggacgacg tccgctgtga taccttcctc ggcgaggatt tttgggatgc gcaccagcgg
      901 cgtgttgcca atggtttcgg cgatgctgtc gtagatgcgc ccgcgcaatg ggcggtcgaa
      961 aacgagaggc ttgtgttcgg atgccatagc ctgttccttt cccgaaccgc cgccgcaacg
     1021 gccggcggcc atgtttggtg ccgcataaaa ggcgagaatt tcgcgcgctg gcaagacgga
     1081 ctgccttgga ggcaaaaaat gatcctcact ggcggttgta tggattttct ttctgttttg
     1141 gtccgctgca atgcaatcgt cgtgctgtga ccgcttggca acacgccgcc cggatgcaca
     1201 tctaccgcat gaacaccttt cgcactgtcc cgctactggt cctgctcctc gccgctgcca
     1261 ccaccggaac tcgtgccgat cccgcccgta ccgccgattc cgcccgtatt ggcgaagcca
     1321 tccgtgccgg gaaagccgcc ttcggcgatt ggcgcggcga tgcaccaggc gtggttcggc
     1381 tcatcacgcc cgacagcctg cccgccccgt tcgccggccg ttcggcggga cagtcgccct
     1441 cggtggtcgg gcgcccggcg ggtgccgccc cgcatgtgcc acccgggttc gaggccacgt
     1501 tgttcgcgac cgggctggag cagccgcgaa cccttcgcac ggcgccaaac ggcgatatct
     1561 tggtcgccga agccggatcc ggccgaatcc gcgtgctacg tgcttccgac ggaacagcca
     1621 aaccggtgca agccgtcacc ttcgcgtccg acctgaacct gccgttcgga ctcgctttct
     1681 ggccgccagg accgtcacct cagttcctct atgtcggcga gaccgggcgg atcgtccgct
     1741 atccgtatca agccggcgat ttgcaggcgc gtggtgctgc gcagaccatc gttgcgagcc
     1801 tgccggaggg cggccattgg acccgcgaca tcgtcttctc gcccgacggc gggcggatgt
     1861 tcgtcgcggt cggatcggag ggtaacctgg ccggcgacct gaccggacaa ccgcccgccg
     1921 gcctgccgct cggcgccgca tggggcgaca acctgaaccg cgccgccgtg ctggagttct
     1981 ctccggaggg cggcaaccag cgcatcttcg ccaccggact gcgcaattgt tcggccgagg
     2041 cgatgcagcc cggcactggt gcgctgtggt gcgcggtcaa cgagcgggat gggctgggcg
     2101 acaatttgcc gcccgattat gcgacgcatg tggaaccgaa cgcgttctac ggatggccgt
     2161 ggttctatat cggcaaccac ccggagccgc ggatgaacgg cgcgcatcgg gatatcgccg
     2221 gtcgcgtgac ggtgccggat gtcctgatcc agccgcactc cgccccgctg ggcatcgtgt
     2281 tctacgatgg cgcgcagttt cctccggcct atcgtggcga tgccttcgtc gcgctgcatg
     2341 ggtcgtggaa ccgggcaaag cgcaccggct acaaggtggt gcggctgccg cttcagaacg
     2401 gccggcctaa cggcacctac gaggatttcc tgacgggttt cgtcgcctcg gaccaggcag
     2461 tgtgggggcg acccgtcggg ctgaccgtgg cccgggacgg ttcgctgctt gtcagcgaag
     2521 atgccaacgg cacgatatgg cgggtcacct atcggcatac gccatgatcg gccattcggc
     2581 ctcggtccgg ttcatcctcg tcacactggt gatcgactcc ctggggttcg gcctggtggt
     2641 gccgatcgtg ccgtcattgg tgctgcaatt gtctgggctg accgtatcgg ctgcctcgat
     2701 gtgggttggg gcgctgctga cggcattcgc gctgatgcag ttcgcctgcg ccccggtact
     2761 gggcgctttg agcgaccgct tcggccgccg ccccgtgctg ctgctgtccc tcggcggaat
     2821 ctgcgccaac tacctgttgc tggcatgggc gccatcgctg gcctggctgt ttctgggacg
     2881 gctgatcggc ggagcgaccg cggcgaacgc ggccaccgcc accgcctaca tcgccgatgt
     2941 gattccaccg ggcaaacggg ccggccagtt cgggttggtc ggcgcgatgt tcggcatcgg
     3001 cttcatcgcc ggaccggcac tgggcggcgt tttgggtgcc tatggcctgc gtttgccgtt
     3061 cgtggcggcc tcgatattgg cgggatgcaa cgtgctgtat gggttgttcg tgctgccgga
     3121 aagcctgccg ccggcccgcc gccgcaagat cgactgggga cgggccaacc cattgggctc
     3181 gctgcgcgtc ttgatggcaa accgcgacct gggacgtctc gccatcgcct ggggcagcat
     3241 ctggttcgcc ctgggcgcgc tgcaaaccac cttcgtattg gccaatgagg tcaggttcgg
     3301 ctgggactcg cggcacaacg gactggcact ggcgttggcc ggtttgggat cggcgctggt
     3361 gcaaggcctg ctggtgcgcc gcgtcgtgcc ctggctggga gaacgccggg cggcgatgat
     3421 cggcgggatg attaccgcgg tggcgtattt gtgcatcgcc ttggcgcctg tcggttgggt
     3481 ggtgctgctg gggatcgtcc tgcaatccgt aggggcgatc accagtccgg cggtacaggg
     3541 catggtgtcg gctctcgccg cggcagaccg gcagggagaa acacagggag ccttggcgtc
     3601 ggtgcaaggt ctgacggcta tcgcgtcgcc gctggtcgcg agttgggtgt tctctacgtt
     3661 caccgccgtc gcgacgccga tctttctgcc aggggcccca tttgtggtct cggcgctggc
     3721 gttcggcctg gcgatatggg cggtatcggg ggtacggttg gcgcccgcgt tggtcggcac
     3781 aggcgccggg gttgtggttt cggcc
//
LOCUS       DASWQN010000255         2285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2138170, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000255 DASWQN010000000
VERSION     DASWQN010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138170"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VGC82_04205"
     CDS             <1..1218
                     /locus_tag="VGC82_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422507.1"
                     /translation="RMSKTTPRAIVLGRRHFVAGAGAAGVVSGLAMPAVAQNKPIRMG
                     WIAAVSGMFASNAQAQDWGFRMAVADLNEQGGLLGRKIEIIMRDSAADPSKAVSFVKE
                     LVYNENIDVLCGPINSGEALPTLGIVSGAKKLHLIGGSVEELIDPVKYPLGFRNLNAN
                     GQWIKVAVKFMVEDLKKTKVAIINDNTGYGVLSRDTVTRFLAARNLKPVYTATVDPNK
                     PDVTDELLKAKEAGADVITEWSNATGFVARLVNARGEQNWDVPLVGHPTILQEQVAKL
                     LTKRSYWDNVYGLGYQHEIVDAQGKLPAGVQAFMDKHKDTIAPYMAAGMPAFLQAHAA
                     VTIYAAGLTKAGDTDPFKVKAALESIPVINAPYGPFIYTATDHNGFQDDGIKLVNANA
                     QLPNGGYPPVKIG"
     gene            1252..2100
                     /locus_tag="VGC82_04210"
     CDS             1252..2100
                     /locus_tag="VGC82_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0422508.1"
                     /translation="MIVSGLALGGLYGMTALVYNVMYSTSKVLSFGTGQFAMVGGICT
                     AWLVLDLHWPLWLGFLACLASGGLFGWLSEVIAVRRIVAVSDEHLWVLSTLALSTMVQ
                     EAMAIWWGTDPTPFPRVFPQEFAGAFDQKFWLPLILAVLMAVGLEQFYRRSMIGKLFI
                     AMSEDSFAARARGVATDRMRSLSYILAGMLGALSGFAGGQLTFADFALGTALGLSGFI
                     ALAVGGIGSSVGALVGGAILGMLTSLTTHYFGAEYQKTIAIGLLILLLVVRPQGLFGT
                     TQVRQV"
     gene            2097..>2285
                     /locus_tag="VGC82_04215"
     CDS             2097..>2285
                     /locus_tag="VGC82_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422509.1"
                     /translation="MRHSHIRPAIITVFAVIAVLLPYITGNLYYLHVATVIGVYWVLI
                     ATLNLLVGFTGQLSVGHVG"
BASE COUNT          408 a          669 c          727 g          481 t
ORIGIN      
        1 cggatgtcca aaaccacacc gcgagccatc gtgctcggac gtcgccactt tgttgccggc
       61 gccggcgcgg cgggtgttgt ttcgggcctt gccatgccag cggtcgcgca gaacaagccc
      121 atccgcatgg gctggatcgc cgcggtctcc ggcatgttcg cgtccaacgc ccaggcccag
      181 gactggggct ttcgcatggc ggtggcggac ctcaacgaac agggcgggct gctgggccgc
      241 aagatcgaga tcatcatgcg cgacagcgcc gccgatccgt caaaagccgt cagcttcgta
      301 aaagagctcg tttacaacga aaacatcgat gtgctctgcg gtcccatcaa cagcggcgag
      361 gcattgccca cgctcggcat cgtttccggt gcaaagaagc tgcatctgat cggtggttcg
      421 gtcgaggaac tgatcgaccc ggtcaagtat ccgctgggtt ttcgcaatct caacgccaat
      481 ggccagtgga tcaaggtcgc cgtgaagttc atggtcgagg atctgaaaaa gaccaaggtc
      541 gcgatcatca acgacaacac gggatatggc gtgttgtcgc gcgatacggt gacaaggttt
      601 ctcgccgccc ggaatctgaa gccggtctat accgcgacgg tcgatcccaa taagcccgac
      661 gtcacagacg aattgctgaa ggcgaaagag gctggcgccg atgtcatcac cgaatggtcg
      721 aatgccaccg gttttgtcgc gcgattggtg aacgcgcgcg gcgagcagaa ctgggacgtt
      781 cccctggtcg gccatcccac catcctgcag gagcaggtcg ccaagctgct gaccaagcga
      841 tcctactggg acaatgtcta tggcctcggc taccagcacg aaatcgtcga tgcccagggc
      901 aagctgcccg cgggtgtcca ggctttcatg gacaagcaca aggataccat tgccccgtat
      961 atggcggcgg gcatgccggc cttcctgcag gcgcatgcgg cggtaacgat ctatgccgcg
     1021 ggtctgacga aggcgggcga tacggatccg ttcaaggtca aggcggcgct ggaatcgatc
     1081 ccggtcatca acgcgccata cggcccgttc atttacacgg cgaccgatca caacggcttc
     1141 caggatgacg gcatcaagct ggtcaacgcc aatgcacaat tgccgaatgg tggctacccg
     1201 ccggtgaaga tcggctgact ttaaatcccg gaggacggca ttttgctgct ggtgattgtc
     1261 agcggactgg cgcttggcgg cctgtatggc atgacggcgc tggtttacaa cgtgatgtac
     1321 tcgacgtcga aagtactaag cttcggaacc ggccaatttg cgatggtcgg cggcatctgc
     1381 accgcttggc tggtgctcga cctgcattgg ccgctttggc tcggattcct cgcctgcctg
     1441 gcctcgggtg ggctgttcgg ctggctgagc gaggtcatcg cggtgcggcg catcgtcgcg
     1501 gtatccgatg agcatctttg ggtgctgagt acgctggcgc tttcgacgat ggtacaggaa
     1561 gccatggcga tctggtgggg caccgatccc acgccgtttc ctcgtgtttt tccgcaggag
     1621 ttcgccggcg ctttcgacca gaaattctgg ctgccgctga ttctcgcggt cctgatggcg
     1681 gttggattgg agcagttcta tcgccgctcg atgatcggaa agctgtttat cgccatgtcc
     1741 gaggatagct ttgccgcacg cgcccggggc gtcgcaaccg accggatgcg gtcgttgagc
     1801 tacatacttg caggcatgtt gggcgcgttg tccggcttcg ccggcggaca actgacgttc
     1861 gcggacttcg cccttggcac cgccctgggc ctctccggct tcatcgcgct ggcggtgggc
     1921 ggcatcggca gttcggtggg ggcgctggtg ggcggtgcga tcctggggat gctgacctcg
     1981 ctgaccacgc attactttgg cgccgagtat cagaagacca tcgccatcgg gctgctgatc
     2041 ctgctgcttg tggtgcggcc gcaggggctg ttcggcacga ctcaggttcg ccaggtatga
     2101 gacacagcca catccgccca gccattatca cggtgttcgc ggtcatcgca gtgttgctgc
     2161 cgtatatcac gggcaatctg tattacctcc atgtggcgac ggtgatcggc gtctattggg
     2221 tgctgatcgc aacgcttaat ctgcttgtcg ggtttaccgg tcagctttcg gtcggccacg
     2281 ttggc
//
LOCUS       DASWQN010000256         8153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2401579, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000256 DASWQN010000000
VERSION     DASWQN010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2401579"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VGC82_04220"
     CDS             <1..542
                     /locus_tag="VGC82_04220"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422510.1"
                     /translation="GTRLHGNPMYLGRMMGPHHCSLVSVVVGAAWAALDEFETLMLRN
                     RSLADPTLLWADHVDYQRPFGLALSMTEAAETILHGVAARYMDYCRRWQRDRTPISVE
                     DNLRLWALAQQAGGLARQAVELLFQNSLSAPTKKASRLARYFRDVAMYNSHVSALQNN
                     QATYVARAHFGRPTGFRGL"
     gene            798..2369
                     /locus_tag="VGC82_04225"
     CDS             798..2369
                     /locus_tag="VGC82_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0422511.1"
                     /translation="MSKLTDYTTYADAQRLFSTDRLWELFDGNRERLNIGHECVDRHA
                     VPGRTALRIAHADGSDETITFEELAGWSSRFAHWLAEEGIQQGDRVAIMLEPSLPFYA
                     ALFGVMKRGAVAVPLFTLFGPDGVRLRIRDCEPALLLTTHGKAAALSGLDGPRVLAAD
                     AAFMAALAVFPNRYEPRTASADMAIFQYTSGTTREMPDAIRHTHRAVVVVMNAALYGT
                     GLRPGDRFFCPSSPAWGHGLWHGTLAPLALGIETGAYAGKFAAGRLLKALRDYGSTNL
                     SAAATHYRMMKNHMTEDAGRTANRDMALRKLSFTGEPIDQETSTFIQAAFGAPLCSMY
                     GTTEIGVVLANYPGAADFPIKAGSLGRPVPGVAVEVQRPDGSKCAADELGEIKVRRHD
                     AWFPTKDRGRIDADGYFYHAGRTDDVIISAGWTMSAVEIEEALLGHKAVLAAAVIGVP
                     DPLRGLVVKAFVVPAPGHAPDDATAADIQGFVRNRLSQHEYPRLISFVADLPRTPAGK
                     INRKALREQEAAPVA"
     gene            2442..3584
                     /locus_tag="VGC82_04230"
     CDS             2442..3584
                     /locus_tag="VGC82_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0422512.1"
                     /translation="MNEQLTRHFPKITDKALDALRARRNVRIENTLEPWCHEATRDNI
                     RHYAHGIGDDNPLWCEPAYAAKTRFGGIVALPSFLFATSRIVSGYVGGIPGVHAMWAG
                     ADWTWHMPVLRNQEISTEAWLKDLIEHDTKFAGRAIQQIYHVNFFNPGGDLVAEADSW
                     CFRTDRDQAREQGTKYSDLRARPPRVYTEADLQRLYTHYETEEIRGATPRYWEDVSVG
                     DALPTMAKGPMTVTGFIAYAQGWGGLYIRANKLAWRQISNHPGLGIPNRFGIPDCPER
                     VHWEPEFALMVGAPGAYDYGPERCSWLTHQLTNWMGDDGFLRRASCKIRRHNPEGDLL
                     FIDGKVVRKLREEGRHLVEIEQSAHNQDGELSVLGTGIVELPNRGS"
     gene            3713..5347
                     /gene="acpA"
                     /locus_tag="VGC82_04235"
     CDS             3713..5347
                     /gene="acpA"
                     /locus_tag="VGC82_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEY0422513.1"
                     /translation="MPGDLMMLRLTTLAAITLLTVPPALAVEPNYADIQTVVVIYAEN
                     RSFDNLYGTFPGADGLASASRTAVTQLDRDGKPLRFLPAVWGGTANKVTDGAPVAPVS
                     LTQEQTASFLNRFNHPYDVAALYRSAGTSNVDPLRYTNRDLYHRFYENQMQINGGANN
                     MFAAWADSGGLTMGYFIPTPTKDLPLWGWARRYVLADNFFQGAFGGSFLNHFYLVCSC
                     APYYGHDAHNPNGGKDPVVSIVNADGVTLTTAANSPASAIDGPPVFAASTALTPDFFA
                     VNTMMPPYPPSGNADVEERAVDLKSASTLVPQTATTIGDLLTDSGVGWAYYAGAWDFA
                     QSHAPFAAGTSKANPNFQYHHQPFNYFAKFDPATPEGKANRAAHLLDAGVVTDPTKLP
                     DSRFRDDIRSGTLPPVTFYKPQGSVNEHASYANIADGDHHIDAVLTALRASPQWKHML
                     IVVTYDEFGGWWDHVAPPKADRFGPGTRVPAIIVSPYAKRGTVDHTPYDTTSILRFIT
                     NKWKLPVLPGIAARDEALAARHQAKLGDLTRALVLR"
     gene            5592..>8153
                     /locus_tag="VGC82_04240"
     CDS             5592..>8153
                     /locus_tag="VGC82_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEY0422514.1"
                     /translation="MPVQFSSQDLSRIFDGRALTRGRGLGLAGAVDVQLDGNTITAVV
                     LDCATPFDVRITPALLGRRVVFDHHCSCRVSGCAHLAATAFAALDRFPQLRKPEQQTF
                     LDTLTTPPAEKERQRVVFELAPGEAPHACIVITLLIGERSFTASPTTPRQIAGDEQAD
                     PKLRDLAYLLGEGNQTRTGVAATLVVDLLDALIESGQARWHAGGKRLVKGETRMFASA
                     STAILPPRSGVIVADSGPWYIDALTGAVGRVRIQAPAPPPQPAFVAPVGRGPSDPPKR
                     RLEPATVSDQVIVDRPMIPVLRLTRFPCPDEFGRMQATDALLLEYDYEGVVVPFDDDR
                     QFIRANGPGGPVFVRRDRSAEADAQDAIRRDGLVQMRMASGQAAKGRLVYVFRGSEAA
                     EAWQCFVAERLPALQALGWRNLIDSDFGPRLVKSVGVCDMKVADAPHGAFSLDFGIEI
                     DGARHPLLPILMRLRERGGIAAARVIDGEVVTSLDDGRVLKLPAERIARLLAIMDDLI
                     ETATRSSGDKMVLDASAAPSVLDLEELVTTRWNDGAAIAAHVARYRGGEIPEAPVPSS
                     FTASLRPYQQQGVNWLQHLRAANLGGLLADDMGLGKTAQTIAHIVIEEQAGRLDRPAL
                     VVVPTSLVPNWNAELTRFAPHLRVAVLHGLERHERRRDLTGVHVVITTYTVLARDIEH
                     MAALPWHLVVLDEAQAIKNAASKATHAVCRLDTRHRLCLSGTPIENNLGELWSQFAFL
                     MPGLLGQRKSFNRRFRMPIEKDGDPLRRRQLAARIRPFILRRTKSAVATELPPKHTIL
                     RRITLAPDQREIYETIRATMHEKVTEGIAAKGVAQSHLLVLQALLKLRQVCCDPRL"
BASE COUNT         1477 a         2609 c         2614 g         1453 t
ORIGIN      
        1 cgggcacgcg attgcacggc aacccaatgt atctcggccg catgatgggc ccgcatcatt
       61 gttccttggt ttcggtcgtg gtgggtgccg catgggccgc actggacgag ttcgagaccc
      121 tgatgttgcg aaaccgttcg ctggccgatc caaccctgct gtgggccgat catgtcgatt
      181 atcagcgccc gttcggcttg gcgttgtcga tgaccgaggc agccgaaacc atcctgcacg
      241 gcgtggctgc tcggtacatg gattattgcc ggcgctggca gcgcgaccgt acccccatca
      301 gcgtcgagga taacctgcgg ctctgggcac tcgcgcagca ggccggcggc ctggcccggc
      361 aagccgtgga gttgttgttc caaaactccc tgtccgcgcc aaccaagaag gccagcaggc
      421 tggcgcggta tttccgcgac gtcgcgatgt acaactcgca tgtttcggca ctgcagaata
      481 atcaggcgac gtatgtcgcg cgggcgcatt tcggccggcc gaccgggttt cgtgggctgt
      541 agcctttgcc gccaaggctg gttgaatcgg ttgctgtaag gccgccgcaa aattgacagc
      601 cgacaatccc gagctgctag agcagtttca cggtgactgg aaacgggggt tctctcgcgg
      661 ctgcaaagga agtcgtgggt ggccgcgcca agcacggcca tgacacatag gcgtcgggcg
      721 gcgaaccggc cggcaccttt tccggtcact gtgaaactgc tctagtcgcc gagggcagat
      781 ggggagcacg tcccggcatg tcgaagctga cggactatac gacgtatgcg gacgcccagc
      841 gtttgttttc caccgaccgg ctgtgggagc ttttcgacgg caaccgtgaa cgcctgaaca
      901 tcggccacga gtgtgtcgat cgtcatgcgg tgccaggcag aaccgccctg cggatcgctc
      961 atgccgacgg ctcggacgaa accataacgt tcgaggagct ggcgggctgg tcctcccgct
     1021 tcgcgcattg gctggcagaa gaaggcatac agcaaggtga ccgcgtcgcc atcatgctag
     1081 agccctcgct accgttctac gcagccctgt tcggcgtaat gaagcgcggc gcggtagcgg
     1141 tgccgttgtt tacgctgttt ggtcctgacg gcgtccgctt gcgcatccgg gactgcgagc
     1201 cggccttgct gttgaccacg cacggcaagg cagcagcact ctccggcctg gatggcccgc
     1261 gcgtgctggc ggcggatgcg gcgttcatgg cggcgttggc ggtgttcccg aaccggtacg
     1321 agccgcggac cgcctcggcc gacatggcga ttttccaata tacatccggc acgacacgcg
     1381 agatgcccga cgccatcagg cacactcacc gggctgtcgt cgtggtgatg aacgcggctt
     1441 tgtacggcac tggtctgcgg ccaggcgacc ggttcttctg cccctcctcc cccgcctggg
     1501 gccatggcct gtggcacgga acactggcgc cgctggcgct gggcatcgag accggtgcct
     1561 atgccggcaa attcgccgcg gggcgtttgc tgaaggcgct acgggattat ggttcgacaa
     1621 acttgtcggc cgccgccacg cactaccgca tgatgaaaaa ccacatgacg gaagacgccg
     1681 gccgcaccgc gaaccgtgac atggcattgc gaaagctttc gttcaccggc gagccgatcg
     1741 atcaagaaac gtccacgttc atccaggctg cgttcggcgc gccgctatgc agcatgtacg
     1801 gaacgaccga gattggcgtc gtcctcgcca actatcccgg cgctgctgac ttccccatca
     1861 aggccggttc gcttggccgg ccggttcccg gtgtcgcggt ggaagtgcaa cggccggatg
     1921 gatcgaagtg cgccgctgac gaactgggcg agatcaaggt gcggcgccac gacgcctggt
     1981 ttccgaccaa agaccgaggc cgcatcgacg cggacggata tttctaccac gccggccgaa
     2041 ccgatgacgt gatcatctcg gcgggatgga ccatgagcgc ggttgaaatc gaggaggcgc
     2101 tgttgggaca caaggcggtc cttgccgccg cggtcatcgg cgtgccggat ccgttgcggg
     2161 gcttggtggt caaagcattc gtggtgcccg cccccggtca cgcgccagac gacgcaacgg
     2221 ccgccgatat ccagggcttc gtgcgcaatc ggctgagcca gcatgaatac ccgcgcctca
     2281 tcagtttcgt tgccgacctg ccgcgcaccc cggccggcaa gatcaatcgc aaggcgctgc
     2341 gcgaacagga agccgcgcca gtggcctgat cctgacgctt ggatcacaag cgtcaggtgg
     2401 accggcccgc tgcatcaact tggccggcga acggagaaag catgaacgag caactcacgc
     2461 ggcacttccc gaagataacg gacaaggcgc tcgacgcact gcgcgcgcgg cggaacgtgc
     2521 ggatcgaaaa caccctggag ccgtggtgcc atgaggcaac gcgcgacaac atccgccact
     2581 acgcccatgg catcggcgac gacaacccgt tgtggtgcga gccggcctac gcggcgaaga
     2641 cccgtttcgg cggcatcgtc gcgttgccga gcttcctgtt cgccaccagc cgtatcgtgt
     2701 ccggctatgt cggtggaatt ccaggcgtgc atgcaatgtg ggccggtgcc gactggacct
     2761 ggcacatgcc ggtcttgcgc aatcaggaaa tcagcacaga ggcgtggctg aaggatctga
     2821 tcgagcatga tacgaaattc gccggtcggg cgatccagca aatctaccac gtcaacttct
     2881 tcaatcccgg aggcgacctg gtggcggagg ccgatagctg gtgcttccgc accgaccgcg
     2941 accaggccag ggagcaggga acaaaataca gcgacctccg cgctcggcca ccgagggttt
     3001 ataccgaagc cgaccttcag cgtctctaca cccactacga aaccgaggag attcgcggtg
     3061 ccacgccgcg atactgggag gacgtgtcgg tcggggacgc gttgccgacg atggcgaagg
     3121 gtcccatgac ggtgaccggc tttatcgcct atgcgcaggg ctggggtgga ctatacattc
     3181 gtgccaacaa gctggcatgg cgccaaatca gcaatcaccc tggcctcggc ataccgaacc
     3241 gcttcggcat tcccgattgt ccggaacggg tgcactggga gccggagttc gcgctgatgg
     3301 tcggcgcgcc gggtgcctac gattacggcc cggaacgttg ctcctggttg acgcaccagt
     3361 tgaccaactg gatgggcgac gacggtttcc tgcgccgggc gtcctgcaag atccgccggc
     3421 acaaccccga gggcgacctg ctgttcatcg acggcaaggt ggttcgcaag ctgcgggagg
     3481 aggggcgcca tctggtcgag atcgaacagt ccgcccacaa ccaggatggc gaactctcgg
     3541 tgttgggaac cgggatcgtc gagttgccga accgcggcag ctgacggaaa agcggccgtc
     3601 aggagatgtc atcggcgatg ccttggataa ggggcacgac cgggatcttc atgaccttca
     3661 tccaattgca atggtaaaac cgctgaaatc cgctatgtct gggatgtggc cagtgcccgg
     3721 ggacctgatg atgctgagac tgaccacatt ggccgcgata accctgctga cggttccacc
     3781 ggctcttgcg gtcgaaccga actacgccga cattcagact gtcgtcgtga tctatgcgga
     3841 gaaccgcagc ttcgacaatc tttacggcac cttcccgggc gccgacggtc tggccagcgc
     3901 cagccgaacc gccgtcacgc aactcgatcg tgatggcaaa ccgctgcgct tcctgccggc
     3961 ggtctggggt ggcaccgcca acaaggtaac ggatggcgcg cctgtcgccc ccgtcagcct
     4021 gacgcaggaa cagaccgcga gcttcctgaa ccgcttcaat catccttacg atgtggcggc
     4081 gttgtatcgg tcggcgggga cgtccaacgt cgatccgctg cgctacacca atcgcgatct
     4141 gtatcatcgg ttctatgaga accagatgca gatcaatggc ggtgccaaca acatgttcgc
     4201 ggcctgggcc gattcgggcg gcctgacgat gggatacttc attccaacgc cgacgaagga
     4261 tctgccgctc tggggttggg cgcgccggta cgtgctggcg gacaattttt tccagggagc
     4321 gttcggcggc tcctttctca accacttcta tctggtttgt tcctgtgcac cgtattacgg
     4381 gcatgatgcc cacaacccga atggcggcaa ggaccccgtc gtttccatcg tcaacgcaga
     4441 tggcgtaacg ctgacgacag cggcgaattc gccggcctcg gcgatcgatg gaccgccggt
     4501 tttcgcggca tcgacggcgc tgacgccgga cttcttcgcg gtgaacacga tgatgccgcc
     4561 ctatccgccg agcggaaacg ctgatgtcga ggagcgggcg gtggacctga aatcggcgtc
     4621 cacgctggtg ccgcagacag cgaccacgat cggtgatctg ctgactgatt ccggcgttgg
     4681 ctgggcgtat tatgcgggtg cctgggattt tgcccagtcg catgcgccgt ttgccgccgg
     4741 gacgtcaaag gccaatccga atttccagta tcaccaccag ccgttcaact actttgcgaa
     4801 gttcgatccc gccaccccgg agggcaaggc aaaccgggcg gcgcatctgt tggacgccgg
     4861 ggtcgtcacc gatcctacga agttgccgga ctcacgcttt cgcgatgaca ttcgcagcgg
     4921 tacactgcct ccggtgacct tctacaagcc gcagggttcg gtgaacgagc atgcgtccta
     4981 tgccaacatc gcggatggcg accatcacat cgatgcggtg ctgaccgcgt tgcgagcaag
     5041 tccgcagtgg aaacatatgc tgatcgtggt cacttatgat gagttcggtg gctggtggga
     5101 tcatgtcgcg ccgccgaagg ccgaccgatt tggacctggg actcgcgtgc cggcgatcat
     5161 cgtctcgccg tacgcgaagc ggggaacggt cgatcatacg ccatacgaca cgacgtcgat
     5221 cctgcggttc atcacgaaca aatggaaatt gccggtgttg ccgggaatcg ctgcacgcga
     5281 cgaggcgctg gcagccaggc accaggcgaa gctcggggat ctgacaaggg cgctggtgct
     5341 gcgataacag gcatcccgtt gttctttcat gtctcgccgt atccgatggg tgaagaatga
     5401 tcgccgattc cgtgatataa atctacttat tgttaacaaa tcaagccctt gatggcacca
     5461 cttgatcctc gctgctcagc gggcatgaac gcttgcaatc gccagcaaat cgtggcgtgt
     5521 cataactctt cgaccgcctc agccgactcg gcgcggcggc agatccaaac gcccgaggat
     5581 gcgccgcgct gatgccagtc cagttctcgt cccaggatct cagccgcatc ttcgatggcc
     5641 gagcccttac gcgtggccgc ggcctcggac tggccggagc ggtggatgtc cagctcgacg
     5701 gcaacacgat caccgcagtc gtgctcgact gcgcgacccc gttcgacgtc cgcatcaccc
     5761 ctgccctgct cggacgccgc gtggtgttcg accaccattg ctcctgccgt gtctccggct
     5821 gcgcgcatct ggcagcgacg gcattcgcgg cgttggaccg tttcccccag ttgcgcaagc
     5881 cggaacaaca aaccttcctc gataccctca ccacaccgcc ggcggagaag gaacgccaac
     5941 gagtcgtttt cgaactggcc ccgggcgagg ccccgcacgc ctgcatcgtc atcacgctgt
     6001 tgatcggcga gcgcagcttt actgcctcgc cgacgacacc gcgccagatc gccggcgacg
     6061 aacaggccga tccaaagctt cgcgacctcg cctacctgct gggcgaaggc aatcaaaccc
     6121 gcaccggcgt ggcggcgact ctggttgtcg atctgttgga cgcgctgatc gagagcggcc
     6181 aggcgcgctg gcacgctggc ggaaagcgcc tggtcaaggg cgagactcgg atgttcgctt
     6241 cagcctcgac cgccatcttg ccgccgcggt cgggcgtgat cgttgccgac agcggcccct
     6301 ggtacatcga cgccttgacg ggcgctgtcg gacgcgtccg cattcaggcg ccggccccac
     6361 cgccgcagcc cgccttcgtg gcacctgtgg gccgtggtcc ctccgatccg ccaaagcgcc
     6421 gcctcgaacc agcgacagtc agcgaccagg tcatcgtgga ccggccgatg attccggtat
     6481 tgcggctgac ccggtttccg tgccctgacg agttcggccg gatgcaggcg acggacgcgc
     6541 tgctgctgga atacgactac gagggcgtcg tcgtcccgtt cgacgacgac cgccagttca
     6601 ttcgtgccaa cggccccggc ggaccggtat tcgtgcgccg cgaccgatcg gccgaggccg
     6661 acgctcagga tgccatccgc cgggacggcc tggtgcagat gcgcatggcc agcggccagg
     6721 cagcaaaggg ccggctggtg tatgtcttcc gcggcagcga ggccgctgaa gcatggcagt
     6781 gcttcgtcgc ggaacggctg ccggcgctgc aggcactggg ctggcgcaac ctgatcgaca
     6841 gcgatttcgg cccgcgcctg gtgaagtcgg tcggcgtttg cgacatgaag gtcgccgacg
     6901 cgccgcacgg tgcgttttcg ctcgatttcg gcattgagat cgacggcgcg cgccacccgc
     6961 tgcttcccat cctgatgcgc ctgcgcgaac gcggcggcat cgcggcggct cgggttatcg
     7021 acggcgaggt tgttaccagc ctggacgacg gccgcgtcct gaagctgccc gccgagcgga
     7081 tcgcgcgctt gctggcgatc atggacgacc tgatcgagac ggccacgcgc tccagcggcg
     7141 ataaaatggt gctcgacgcc agcgcggcac cgagcgtcct ggacctggag gagttggtaa
     7201 ccacgcggtg gaacgacggt gcggcgatcg ccgcgcatgt cgcgcgctat cgtggcggcg
     7261 aaatccccga ggcgccggtg ccgtccagct tcaccgccag cctgcggccg tatcagcagc
     7321 agggcgtcaa ttggctgcag cacctgcgcg cagcgaacct cggcggcttg ctggccgacg
     7381 acatgggcct cggcaagacg gcgcagacca tcgcccacat cgtcatcgag gaacaagctg
     7441 gacggctgga tcgcccggca ctggtggtcg tgccgaccag cctggtgccg aactggaacg
     7501 cggaactgac gcggttcgcg ccgcatctgc gcgtcgcggt gctgcatggg ctggaacggc
     7561 acgagcggcg gcgcgacctg accggcgtgc atgtcgtcat cacgacctat accgtgcttg
     7621 ccagggacat cgaacacatg gccgccctgc cctggcactt ggtcgtcctg gacgaggcac
     7681 aggcaatcaa gaacgctgcg tcgaaagcca cgcacgctgt ttgtcggctc gatacaaggc
     7741 accggctgtg cctgtccggc acgccgatcg aaaacaacct cggcgaactc tggtcccagt
     7801 tcgccttcct gatgccgggt ctgctcgggc agcgcaaaag cttcaaccgc cggttccgga
     7861 tgccgatcga aaaggacggc gacccgcttc gccggcggca attggccgcg cgcatccgcc
     7921 ccttcatcct gcggcgcacg aaatcggcgg tggcaaccga gttgccgccg aaacacacga
     7981 tcctgcggcg catcaccctg gcgccggacc agcgcgaaat ctacgagacg atccgcgcga
     8041 cgatgcatga gaaagtaacc gagggcatcg ccgcgaaggg tgtggcgcag agccaccttc
     8101 tggtcctgca ggcgctgttg aagctgcggc aggtttgctg tgatccgcgc ctg
//
LOCUS       DASWQN010000257         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1610469, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000257 DASWQN010000000
VERSION     DASWQN010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610469"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1402)
                     /locus_tag="VGC82_04245"
     CDS             complement(233..1402)
                     /locus_tag="VGC82_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0422515.1"
                     /translation="MDFSLSSELQELQQRTRAFIRDKIIPLESDERQTHHGPTEEFRR
                     ELVALAAAEGLVAPHVGVEYGGMGLSHVGKAVVFEEAGYSLLGPVAMHIFAPDEGNMH
                     LLEAVASTEQKDRWLRPLASGKTRSCFCMTEPAPGAGSDPAAMLTTAVKDGNHYVING
                     DKWFITGAIGAAFAIIMAKDEEGQATMFLADMDTPGIEIVRAMDSLDSAFVGGHAVMR
                     FNNVRVPASDILGEPGKGFRYAQVRLAPARLTHCMRWLGTAKRAHQIATDYALKREVF
                     GSKLVGHEGVGFMLADNEMDIRMSRLSIQHTAWLLDQGERGGTESSMAKVFCSEAIWR
                     VVDRCVQVLGGQGVTGETLVARIFNEVRGFRIYDGPSEVHRWSLAQRIARNGANW"
     gene            complement(1402..>2102)
                     /locus_tag="VGC82_04250"
     CDS             complement(1402..>2102)
                     /locus_tag="VGC82_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEY0422516.1"
                     /translation="EHLPAELAHRPEIGARLSQVLLETIAAIHAVDTKAVGLDDLGRP
                     AGFLARGVAGWRKRGLTAMEDGTERLHTDVGSWLERHIVPDGSPALLHNDFKLNNIIL
                     DPDDLSPRAVVDWDQGTRGDPLFDFATLISYWIHDDDPPAMHDMEQMPAVEACLCPRQ
                     QAVAEYGRLSGRDMSDFLFHRVLAMYKLSVIFLQLGLRYRSGATQEPRYAPLTGIGTG
                     ILEFTHEIAQGRAF"
BASE COUNT          382 a          708 c          628 g          384 t
ORIGIN      
        1 ggtgcgccag ccgatacaaa ttactcgatg atagcgatcc tgtcgtcgac tggcacggcc
       61 gcgttttgga cctgtacggc ggccttgggc ggtccgccaa gcgggcataa attccaatgg
      121 agcccgcccg cgtgcggact ggctgttggt gtagctgtta gccgccgcct tccaggccgg
      181 tcatcggaga ttcggactcc actcacaggc cccagtcaac aggccctgat cgctaccagt
      241 tcgctccatt ccgcgcgatg cgctgagcca ggctccagcg atgcacctcc gacggaccgt
      301 cgtagatgcg aaagccacgc acctcgttga agatacgggc caccagggtt tcccccgtca
      361 cgccctgccc gcccagtact tgcacgcaac gatcgaccac gcgccagatc gcttcggaac
      421 agaatacctt cgccatcgac gattcggtgc cgccgcgttc gccctggtcc agcaaccacg
      481 ccgtgtgctg gatcgacagt cggctcatgc ggatgtccat ctcgttatcg gccagcatga
      541 agccgacgcc ctcgtgcccg accagtttgg agccgaacac ttcccgcttc agcgcatagt
      601 ccgtcgcgat ctggtgcgct cgtttggcgg tgcccaacca gcgcatgcag tgggtcaacc
      661 gcgccggggc cagccgtacc tgagcatacc ggaacccctt ccccggctcg cccaggatat
      721 cgctggccgg cacgcggaca ttgttgaacc gcatcactgc atggccgccg acaaacgcgc
      781 tgtccaaact gtccattgcc cggacgatct cgatccccgg cgtgtccatg tcggccagga
      841 acatcgtcgc ctgaccctcc tcgtccttgg ccatgatgat ggcgaaggcc gcgccgatcg
      901 cgccggtaat gaaccatttg tcgccgttga tgacgtagtg attgccgtcc ttcaccgccg
      961 tcgtcagcat cgccgccggg tcggatcccg cgcccggtgc cggctccgtc atgcagaagc
     1021 acgaccgcgt cttgccggaa gccaacggcc gcagccagcg gtctttctgt tcggtggaag
     1081 ccacggcttc cagaagatgc atgttgcctt cgtcgggggc aaaaatatgc atcgcgaccg
     1141 gaccgagcag cgagtatccg gcttcctcga acaccacggc cttgccgaca tgggacaagc
     1201 ccataccgcc gtactcgacc cctacatgcg gtgcgaccaa gccctctgcg gcggccagag
     1261 ccaccagctc gcggcgaaat tcttccgtgg ggccgtgatg ggtctgccgc tcgtcactct
     1321 ctagcggaat gattttatcg cggatgaagg cgcgtgtccg ctgctgcagc tcttgcagtt
     1381 cggacgacag ggagaaatcc atcagaatgc gcgtccttgg gcgatctcgt gggtgaactc
     1441 cagaattccg gtaccgatcc ccgtcagcgg agcatatcgc ggctcctgcg tcgcaccgga
     1501 tcgatagcgc aagccgagtt gaaggaagat caccgacaat ttgtacatgg ccagcacgcg
     1561 gtgaaacagg aaatcggaca tgtcgcggcc ggacagtcgg ccgtattcgg cgactgcctg
     1621 ctgacgcggg cacaggcacg cctcgactgc gggcatctgc tccatgtcgt gcatcgccgg
     1681 gggatcgtcg tcatggatcc agtaactgat cagggtggca aaatcgaaca gcggatcgcc
     1741 ccgcgtgccc tggtcccaat cgaccacggc gcgcggcgat aaatcgtctg ggtccaggat
     1801 gatgttgttc agcttgaagt cgttatgcag cagcgccggc gacccatcgg ggacgatatg
     1861 gcgttccagc cagctaccga catcggtatg aaggcgttcg gtgccgtcct ccatcgccgt
     1921 cagcccccgc ttgcgccagc cggcgacgcc acgcgccagg aacccggccg gccggccaag
     1981 gtcgtcgagc ccgaccgcct tggtatccac cgcatggatg gccgcgatgg tctccagcag
     2041 cacctgcgat aggcgggcgc caatttccgg acgatgagcc agttctgcgg gcaaatgctc
     2101 gc
//
LOCUS       DASWQN010000258         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_686507, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000258 DASWQN010000000
VERSION     DASWQN010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686507"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1731
                     /locus_tag="VGC82_04255"
     CDS             127..1731
                     /locus_tag="VGC82_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422517.1"
                     /translation="MRIALSAAWFGALFAAAATVPAQSQETPKRGGTLTYMIPADGGP
                     SLDGHRETTFAVLHATAPFYSVLVRINPDNPASTTDFVCDLCTDMPAPTEAGLTYTFQ
                     IRQGVRFHDGTPLSAHDVAASWRKIVSPPKGVASARQNNFVMVDKIEDPDDRTVIFRL
                     KFATLSFLPALADPYSFIYSKQILDRDMHWYEKNIMGSGPFKFESYDIGQSVKGLRNP
                     DYYREGQPYLDGFTAIFAPKQAVRVDAIRGDRAGLEFRGLPPAARDQLVKELGSKITV
                     QESDWNCGNVLTPNHRKKPFDDVRVRKALFLAIDQWRGAAALQKIANIRTVGGIAFPG
                     SPFAATKEELQQIPGYWPDIEKSRAEARRLLKEAGQENLSFELLNRNVDQPYVIVGTW
                     LVDEFSKIGVKVTQKIMPTAPWQEAMRAGDFTVALEANCQNIVNPIADTGRYLQHELY
                     SENFAYHDDPVQAALYDKMVHETDVAKARLLMRDYERQTLDIGAHQLMVTWWYRIVPI
                     RSYVKGWKISPSHYLNQDLANIWLDK"
     gene            1731..>2198
                     /locus_tag="VGC82_04260"
     CDS             1731..>2198
                     /locus_tag="VGC82_04260"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0422518.1"
                     /translation="MYRYILKRVLLVIPTLIGAAALVFLLMRLIPGDICVVRLGSGGS
                     FDPRALAACHAELGMDRSVLIQFIDFLWGFFRLDFGTSMWSGKPVTTEILARLPISLE
                     IAILATVVAIAIAIPLGALSALKQNTWLDVAVRVFAIGGIATPSFWIGIVSILL"
BASE COUNT          414 a          650 c          711 g          423 t
ORIGIN      
        1 ctggggcatg atcacgctga gcggaacgtt cagagcctgt gaatcatgcg atcaaacaag
       61 atattggagc atttgcagcc ggcatggtca ggctgcgaat gttctgaacg gggagggcgc
      121 gcgaccatgc ggattgcact atcggcagcc tggttcggcg cgctattcgc ggcggccgct
      181 acggtgcctg cccagtcgca ggaaacgccg aagcgcggcg gcacgttgac ctatatgatt
      241 ccggccgatg gcggcccaag cctggatggc catcgcgaaa cgacgttcgc ggtgctgcac
      301 gccacggcgc cgttctacag tgtcctggtg cggatcaatc cggacaatcc tgcctcgacg
      361 acggacttcg tctgcgatct ctgcaccgat atgccggctc cgacagaggc cggcctgacc
      421 tacacttttc agatccggca gggggtcagg ttccacgacg gtacaccgtt gagcgcccat
      481 gacgtggcag cgagctggcg caagatcgtc agcccgccga agggcgtcgc gagcgcacgc
      541 cagaacaatt tcgtgatggt agataaaatc gaagatccag atgacaggac tgtgatcttc
      601 cgcctcaagt ttgccacgct gtcgttcctg ccggcgctcg cggatccata ctcctttatc
      661 tactccaaac agattctcga tcgggatatg cactggtacg agaagaacat catgggctcg
      721 ggtccgttca agttcgaaag ctacgacatc ggccagtcgg tgaaggggtt gcgcaacccg
      781 gactactacc gcgaagggca accgtatctg gacggcttca ccgcgatctt cgcgccgaag
      841 caggcggtgc gtgtcgatgc aatccggggg gatcgggcgg ggttggaatt ccggggcctg
      901 ccgcccgctg cgcgcgacca gttggtgaag gagctgggca gcaagatcac cgtgcaggag
      961 agcgattgga actgcggcaa cgtgctgacg ccgaaccacc ggaagaagcc gttcgacgac
     1021 gtgcgggtgc gcaaggcgct gttcctggcg atcgaccagt ggaggggtgc ggccgcgctg
     1081 cagaagatcg ccaatatccg cacggtcggc ggcatcgcgt ttcccggatc gcctttcgct
     1141 gccaccaagg aagagctgca gcagattccg ggctattggc cggacatcga gaaatcccgt
     1201 gccgaggccc gccggttgct gaaggaggcg gggcaggaga acctgtcgtt cgagctgctc
     1261 aaccgcaacg tggatcagcc ctatgtcatc gtcggcacct ggctggtgga cgagttcagc
     1321 aagatcggcg tgaaggtcac gcagaagatc atgccgacgg cgccgtggca ggaagcgatg
     1381 cgggccggcg acttcaccgt ggcgctggag gccaattgcc agaacatcgt gaatccgatc
     1441 gccgataccg gccgttactt gcagcatgaa ctgtacagcg agaattttgc ctatcatgac
     1501 gacccggtac aggcggcgct gtacgacaag atggtccacg agaccgacgt cgcgaaggcg
     1561 cggttgctga tgcgcgacta cgagcgccag accctcgaca ttggcgccca ccagttgatg
     1621 gtgacctggt ggtaccgcat cgtgccgatc cgctcctacg taaagggctg gaagatcagt
     1681 cccagccatt acttgaacca ggatctggcg aatatctggc tggacaagta atgtaccggt
     1741 atattttgaa gcgcgtgctg ctggtgatcc cgacgctgat cggggcggcg gcgctggtgt
     1801 tcctgttgat gcggctgatc cccggcgaca tctgcgtcgt gcgccttggc tccggcggca
     1861 gcttcgaccc gcgcgcgctt gccgcctgcc atgccgaact gggcatggac cggtcggtgt
     1921 tgattcagtt catcgacttc ctttgggggt ttttccggct cgacttcggc acttcgatgt
     1981 ggtcgggcaa gccggtgacg accgagatcc tggcgcggct gccgatctcg ctggaaatcg
     2041 ccatcctggc caccgtcgtc gccatcgcga tcgccattcc gctcggtgcg ctctcggcgc
     2101 tgaagcagaa cacctggctc gatgtggcgg ttcgtgtatt cgccatcggc gggatcgcga
     2161 caccgtcatt ctggatcggc atcgtctcga tcctgctg
//
LOCUS       DASWQN010000259         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2402026, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000259 DASWQN010000000
VERSION     DASWQN010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402026"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1831)
                     /locus_tag="VGC82_04265"
     CDS             complement(<1..1831)
                     /locus_tag="VGC82_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422519.1"
                     /translation="MSRKQRDAGAGSMRVETPPPANDTTEPAAPEPAEDHAPPLPADP
                     FDSEPEDADAPISIEWPIGQPARQQAPEPPIATPIPQHFWFNAEPAAAIAEAPKILID
                     IDQEVSAALVFNRFDLKLRGRVVSAAAIDELELLNGEDTIVRMNFGKDHSATQVPLDG
                     NELALQHDYLITLPRLQEQAAEPCKLTLRARTDDGHVHDQAFELAIDLNQPKPVTVVS
                     GPTRPSRTDVWPHIVLYVERALVGSDGALMVLGWTIASTPIFTVQGFTAEGERLPAAR
                     IGLPRDDVASVYPAYPQAGMAGFSMSARLDPAKAAELTAVRVQSICRNGYSHEVTVPV
                     ERVASIAATLGPPAPIPGKAPPVEPRSPKPQPDFLRDPRRKIYYYGDIARVALDGTVT
                     VLGWAVCAVGIANIGIYIDGVKVGDAQLALPRPDIAGEFPNNPMAHLSGFRYEARVPE
                     LAGGIHELLIVVRNGMDDVLEETDTITFNPPVEPRSAPSAEPSEFRFELDNPRVVDGV
                     MAEPVTGRLTIEGWVVAQSGISHIEVRLGGQHLAQAHYGLARQDVGIAFPEMPSSLRS
                     GYAFHCPPRSLRSGTHVVTLTVHAKNGMELVHRFDIEVKRPDGE"
     gene            complement(2038..>2853)
                     /locus_tag="VGC82_04270"
     CDS             complement(2038..>2853)
                     /locus_tag="VGC82_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422520.1"
                     /translation="EQRFAEVESDPALIPDHAILQTWMPYPTHMLPETQPGTMTWLVN
                     RYRTAQTRLDLHRTGDRLQGTVTDTAGQPLAGAPVTLSADLPSESGAPIAHTRSGHVP
                     PKAASALVALRINAECNCSGPADIAIAPMRFHNDRTNETVQRAFQPAKPNTNAEPSRF
                     QAQPGQSVTQNTPAFPVTADDPFTIQVPMRTDLASAGSGYVALIFLNAAGKEIMRLRL
                     PFDPVQRPIGTLTTDAHGGFSLLPDPATLRASVGFHAEFPGDPQHRTASATIR"
BASE COUNT          434 a          856 c         1035 g          528 t
ORIGIN      
        1 cctcgccgtc cggacgcttg acctcgatat cgaagcggtg gaccagctcc atgccgttct
       61 tcgcatgcac cgtcagcgtt acgacatggg ttcccgagcg cagcgatcgg ggtggacaat
      121 ggaacgcgta gccactgcga agcgaactgg gcatttccgg gaaggcgatg ccgacgtcct
      181 ggcgtgccag cccataatgc gcctgagcaa ggtgttggcc gcccagccgc acctcgatat
      241 ggctgattcc ggattgcgcc accacccagc cctcgatcgt cagccggccg gtcactggtt
      301 ccgccatgac gccgtcaacc acgcgcgggt tatcgagttc gaagcgaaac tcggacggtt
      361 ccgcggaggg cgcggatcgt ggttcgaccg gagggttgaa ggtgatcgtg tcggtttctt
      421 ccaggacatc gtccataccg ttgcgcacca cgatcagtag ctcgtggatt cccccggcga
      481 gctcgggaac ccgcgcctcg taccggaagc cggaaaggtg cgccatggga ttgttcggga
      541 attcgccggc gatatccggc cttggcaggg ccaactgggc gtcgccgact ttcacgccgt
      601 cgatgtagat accaatgttg gcgatgccga ccgcgcacac cgcccacccg agcacggtga
      661 ccgtgccgtc cagggccacg cgggcgatgt cgccgtaata atagatcttg cggcgtgggt
      721 cgcgcaggaa atccggctgc ggtttgggcg aacgcggctc cactggcggg gctttgcccg
      781 ggatcggggc cggtggcccg agcgtcgcgg cgatgctggc cactcgttcc accggcaccg
      841 tcacttcgtg cgagtagcca ttccggcaga tcgactgcac ccgcaccgcc gtgagttcgg
      901 cggcctttgc tggatccagg cgcgcggaca tcgagaaacc cgccattccg gcctgcggat
      961 aggcgggata gacggacgcg acgtcgtccc tgggaagacc gatcctggcg gccggcaggc
     1021 gttcgccctc cgccgtgaag ccctggacgg tgaaaatcgg cgtcgatgcg atcgtccagc
     1081 ccagcaccat aagcgcgccg tccgacccga ccagcgcacg ttcgacgtac agtacaatat
     1141 gcggccatac atcggtgcgg ctcggccgcg tcggaccgga caccacggtt accggtttgg
     1201 gctggttcag atcgatcgcc agttcgaacg cctggtcgtg gacgtggccg tcgtcggtgc
     1261 gggcccgcag cgtcagcttg catggttcgg ccgcctgctc ttgcaggcgc ggcagggtta
     1321 tcaggtaatc gtgctggagc gccagctcgt taccgtccag cggcacttga gtcgccgaat
     1381 ggtccttgcc gaaattcatc cgaacgatcg tatcttcgcc gttcagaagc tccagttcgt
     1441 cgatcgcggc ggccgagacg acacgcccgc gcagtttcag gtcgaagcgg ttgaaaacga
     1501 gcgcggccga tacctcctgg tcgatgtcga tcaggatctt cggcgcctcg gctattgcgg
     1561 ccgccggttc ggcgttgaac cagaaatgtt gcgggattgg cgtggcgatc gggggttcgg
     1621 gcgcttgctg acgcgccggc tggccgattg gccactcgat gctgatcggt gcatccgcat
     1681 cctcgggctc ggagtcgaat ggatcggcgg gtaacggtgg cgcgtgatcc tccgccggct
     1741 ccggcgccgc gggctcggtc gtatcgttgg cgggcggcgg agtctcgacc cgcatgctgc
     1801 cggccccggc gtcgcgttgt ttacggctca tgaaggttcc gctgtcagca gtggtttatt
     1861 ccgcgccagt catgggttcg ctcgggccaa agacgaccgg gtcatcgcgc cccagcgaca
     1921 gccatcgttt gccggtgatc gattatccct cgttccatgc tcggcttttt atccgaagcc
     1981 aaacgaaata gcacgaaaaa gtaacccatg tcgttttggg gccgagcggt tgcggcctta
     2041 gcggatcgtg gcggaggcgg tgcggtgctg ggggtcgccg gggaattcgg cgtggaagcc
     2101 gacgctggcg cgcagcgtgg cgggatcggg cagcagcgaa aagccgccat gcgcatcggt
     2161 cgtgagcgtg ccgatcggac gctggacggg gtcgaacgga agccgcaggc gcatgatctc
     2221 cttgccggcg gcgttgagga agatcagcgc cacgtagccg ctgccggcgg aggcgaggtc
     2281 ggtgcgcatc ggcacctgga tggtgaacgg atcgtctgcg gtgaccggga aggccggcgt
     2341 gttctgcgtg acggactgtc ctggctgggc ctggaagcgg gagggttcgg cgttggtgtt
     2401 cggtttggcg ggctggaagg cgcgctgcac cgtctcgttg gtgcggtcgt tgtggaagcg
     2461 catgggggcg atggcgatgt cggcgggacc cgagcagttg cactcggcgt tgatgcggag
     2521 tgccaccagg gcggaggcgg ccttcggcgg gacgtggccg gagcgcgtgt gcgcgatggg
     2581 ggcgccggat tcactgggga ggtcggccga cagggtgacc ggcgcgccgg ccagtggctg
     2641 cccggcggtg tcggtgaccg ttccttgcag gcggtcgccg gtgcggtgca ggtccaggcg
     2701 tgtctgcgcg gtgcggtaac ggttgaccag ccaggtcatg gtgcccggct gggtctccgg
     2761 cagcatgtgg gtggggtatg gcatccaggt ttgcaggatg gcgtggtcgg ggatgagtgc
     2821 tgggtcggac tcgacctcgg cgaaacgctg ttc
//
LOCUS       DASWQN010000260         6439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_423131, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000260 DASWQN010000000
VERSION     DASWQN010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423131"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VGC82_04275"
     CDS             <1..856
                     /locus_tag="VGC82_04275"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEY0422521.1"
                     /translation="YTMPGFATSDHTKSNAWALMKSLGVTASELDIRPAAKQMLTDLG
                     HGYATGQSHYDITFENVQAGLRTDYLFRLANHHGGMVIGTGDLSELALGWCTYGVGDQ
                     MSHYNVNAGLPKTLIQHLIRWIIGSKRFADDVLATLDAILATEISPELVPVGEGETPQ
                     STEAKIGPYALQDFTLFHILRYGFVPSKIAFMALHTWEDPARGDWPPHFHADRRVAYD
                     LKQIRHWMEVFLTRFFAFSQFKRSAMPNGPKVSAGGSLSPRGDWRAPSDGNAAAWLAE
                     LERNVPAE"
     gene            864..1610
                     /locus_tag="VGC82_04280"
     CDS             864..1610
                     /locus_tag="VGC82_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0422522.1"
                     /translation="MPHFLPLVLAGLLAGTMNAVAGGGSFVSFPVMVLVGLPPIAANA
                     TSTVALFPGTMASTWAYRDDLRGIAGISLKLLLPISLLGGMIGAILLLITPSGAFDLV
                     IPWLLLLATLTFLGGRHLGDWLRRYIRIGRPAFLAIQFILSIYGGYFGGAIGLMMMAV
                     WTLIDTTELHMMAPTRTLLVSATNGSAVLCFALAGAVHWPEMLAMMVSAIVGGYYGAR
                     FARHLPPTVLRWFVILLSATVTAVFFHRRY"
     gene            complement(1644..2924)
                     /locus_tag="VGC82_04285"
     CDS             complement(1644..2924)
                     /locus_tag="VGC82_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0422523.1"
                     /translation="MTPRTNPGKFGLRSSRASRTMALSRSTRVNILIGNGHFLSHFYV
                     LCLPTTFLAWQSAFNVSFAELGMSVMLMSGTTAILQTPVGFLVDRFGARPFLIGGTLL
                     MSVSLACMGLVTQFWQILALATLSGIGNSVIHPADYAILSGSVDRDRMGRSFALHTFS
                     GNLGFSAGPPVTALLMTAFGWRTTLLTVGLLGLPVVLSIILQSRILKDQVRIPAHDAV
                     RMSGRDLLTSRTMILFFMFFMLGAMAGGGVQSWLVTVLHTFKGIELKIAATALTAYTL
                     GSTTGVLVGGWFADTFKHHILPFVISLTILSALLMLGIDGLTLPAVAIIGMTFMSGLA
                     LGASRTPRDVMVKDAAPPGQIGKVFGFVSAGLPLGSAITPVPFGMLIDKGHPELVLAL
                     VAVLLLLSLFCAGSAKASANVSARTSGTAVQPAE"
     gene            complement(3006..3080)
                     /locus_tag="VGC82_04290"
     tRNA            complement(3006..3080)
                     /locus_tag="VGC82_04290"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3046..3048),aa:Thr,seq:tgt)
     gene            3409..4920
                     /locus_tag="VGC82_04295"
     CDS             3409..4920
                     /locus_tag="VGC82_04295"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEY0422524.1"
                     /translation="MNMKPQRRNVHKIAFAATLLAGTSLGGLALSHGAWADDATAVNP
                     PGAQLQPHTLPDFTNLVTQVKPAVVSITNKMKATPVALEQGQQGGEMQRLPFPFNQMI
                     PNGPQQQDRAVEARGSGFIISADGTIVTNNHVIKGARTLSVTLDDGTVLPAKVVGSDP
                     RTDIAVLKVSASRPLPFIQLGNSRDVKPGEWVVAMGNPFGLGGSVTAGIVSAVSRDIG
                     AGPYDQFIQVDAPINQGNSGGPLFTQDGKVIGMNTAILSPTGGSVGIGFAVPSDMIRT
                     VTAQLEKTGTVVRGYVGVEAQPITPSTARALHLKENDGALLAGVQPNSPAAEAGLQPG
                     DVIQAVNGVKVANPKELALNVANIQPNDQAHLSVLHDGQTRDVTVKVGTLPNEQTASN
                     DNPAGGERRARIGLALAPLSPDMRDQLDVPEGTKGAVVQGVQPGSPAEVAGLQPGDVI
                     VGVGTRAVTSPAEAARAMQTALNGNDHAMALRVIRNGQPIFVGVTVDDQNNQG"
     gene            complement(5279..5575)
                     /gene="minE"
                     /locus_tag="VGC82_04300"
     CDS             complement(5279..5575)
                     /gene="minE"
                     /locus_tag="VGC82_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEY0422525.1"
                     /translation="MSLFELFRKKTTAPVARDRLQVMLAHERSIIGKSDLLATLQEEI
                     LAVIAKHISVDRDAVQIKLDRGASFSTLEIDVEVPLNAAKTGRPAGGAGVPASA"
     gene            complement(5572..6387)
                     /gene="minD"
                     /locus_tag="VGC82_04305"
     CDS             complement(5572..6387)
                     /gene="minD"
                     /locus_tag="VGC82_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEY0422526.1"
                     /translation="MGKVLVVTSGKGGVGKTTSTAALGAALARGGENVVVIDFDVGLR
                     NLDLVMGAERRVVFDLINVVQGDAKLAQALIRDKRIDTLSLLPASQTRDKDALTMEGV
                     DKVIGELKEKFDWVICDSPAGIERGALLAMRNAEMAVVVTNPEVSSVRDSDRIIGMLD
                     SKTLRAERGERIDKFLLVTRYDAGRAARNEMLKVEDVLEILSIPLLGIIPESEEVLKA
                     SNIGSPVTLNNPNSAPSRAYFDAAKRLKGETLDVVIPMQRSGFISRLFGRRVA"
BASE COUNT         1215 a         2047 c         2020 g         1157 t
ORIGIN      
        1 ctacaccatg ccgggcttcg ccacctcgga tcataccaaa tccaacgcct gggcgttgat
       61 gaagtcactg ggggtgaccg caagcgagct cgacatcagg ccggctgcga agcagatgct
      121 gacggatctc gggcatggct acgccaccgg ccagtcgcat tacgacatca cgttcgagaa
      181 cgtgcaagcc gggctgcgca ccgactacct gttccggctc gcgaaccacc atggcggcat
      241 ggtgatcggt accggcgatc tgtcggaact cgcgctcggc tggtgcacct atggcgtcgg
      301 cgaccagatg tcgcactaca acgtcaacgc cggcttgccg aagacgctga tccagcacct
      361 gatccgctgg atcatcggca gtaagcgctt tgccgacgac gtgctggcaa cgctggatgc
      421 catcctggcc acggaaattt cgccggaact ggttccggtt ggcgagggtg agacgccgca
      481 aagcaccgag gcgaagatcg ggccgtatgc gctgcaggat ttcaccctgt tccacatcct
      541 gcgctacggt ttcgtgccct cgaagattgc cttcatggcg ttgcacacct gggaagaccc
      601 cgcgcgcggc gactggccac cccatttcca tgctgaccgg cgcgtggcct atgaccttaa
      661 gcagatccgg cactggatgg aggttttcct gacgcgcttc ttcgccttca gccagttcaa
      721 gcgcagtgca atgccgaatg gcccaaaagt gtcggccggc gggtcgctgt cgccgcgggg
      781 cgactggcgc gctccttcgg acggcaacgc ggcggcctgg ctggccgagc ttgagcggaa
      841 cgttccggcc gagtagctca gccgtgccac atttccttcc ccttgtcctg gccgggttgc
      901 tggcgggaac gatgaatgcg gtcgccggcg gcggctcttt cgtgtcgttc ccggtgatgg
      961 tgctggtcgg gctgccgccg atcgcagcca acgccaccag cacggtggcg ttgtttcccg
     1021 gcaccatggc gagcacctgg gcgtaccggg acgacctgcg tggcatcgcc gggatcagtt
     1081 tgaagctgct gctgccgatc agcctgttgg gcgggatgat cggcgcgatc ctgctgctga
     1141 tcacccctag cggcgcgttc gacctggtga tcccctggct gctattgctg gcgacgctga
     1201 ccttcctggg tggccggcac ctgggggact ggctgcgccg ctacatccgg atcgggcggc
     1261 cggcattcct ggcgatccag ttcatcctgt cgatctatgg cggctatttc ggcggcgcca
     1321 tcgggctgat gatgatggcg gtgtggacgc tgatcgacac cacggaactt cacatgatgg
     1381 cgccgacccg gaccttgctg gtcagtgcca ccaacggttc cgctgtgctg tgcttcgcct
     1441 tggcgggagc ggtacactgg ccggagatgc tcgcgatgat ggtttcggcc atcgtcggcg
     1501 gctattatgg cgctcgattc gcacggcatt tgccgccgac tgtgctgcgc tggttcgtca
     1561 tcctgctgag tgcgaccgta acggcagtct tctttcaccg ccgctactga gacttgcagg
     1621 ggctactgag acttgcggcg gcgctactcc gccggttgca ccgctgtgcc ggaggtccgg
     1681 gccgacacgt tggccgatgc cttggccgat ccggcgcaga acaggctgag cagcagaagc
     1741 actgcgacca gagccagcac caactccgga tggcccttgt cgatcagcat tccgaacggc
     1801 accggcgtga tcgcacttcc cagcggcagc ccggcgctga cgaagccgaa gaccttgccg
     1861 atctggcctg gcggggcggc atctttcacc atgacgtcgc ggggagtccg gcttgcaccc
     1921 agcgccaggc cggacatgaa ggtcatgccg atgatagcca ccgccggcag ggtcagccca
     1981 tcgatgccga gcatcagcaa cgcggacagg atggtcaggc tgatgacgaa aggcaggatg
     2041 tggtgtttga aggtgtcggc gaaccagccg ccgaccaata cgcccgtggt ggatcccagg
     2101 gtgtaggcgg tcagcgcggt ggcggcgatt ttcagctcga tccctttgaa cgtgtggaga
     2161 acggtcacca gccaggactg caccccgcct ccggccatgg cgcctagcat gaaaaacatg
     2221 aagaacagga tcatggtgcg ggaggtcagc aggtcccggc cggacattct caccgcatcg
     2281 tgggccggga tgcgcacctg gtccttgagg atgcggcttt gcaggatgat cgacagtacg
     2341 accggaaggc cgagcaggcc cacggtcaac agggtggttc gccagccgaa agccgtcatc
     2401 agcaaggcag tcaccggtgg gccggcagaa aagccgaggt tgccgctgaa ggtgtgcagc
     2461 gcgaaggacc gccccatgcg atcccggtcc acggaacccg agaggatggc atagtcggcg
     2521 ggatggatga ccgagttgcc gatgcccgac agcgtagcca gcgccaggat ctgccagaac
     2581 tgggtgacca gccccatgca ggccagggac accgacatca gcagggtgcc gccgatcagg
     2641 aatgggcggg ccccgaaccg gtccaccagg aagccgacgg gggtttgcag gatggcggtg
     2701 gtgccggaca tcagcatgac gctcatgccg agttcagcaa agctgacgtt gaacgcgctc
     2761 tgccaggcca agaacgtggt gggaaggcag aggacgtaga aatgcgagag gaaatgcccg
     2821 ttgccgatca ggatgttgac gcgggtgctg cggctgagcg ccatggtgcg ggaagccctg
     2881 gatgaacgaa ggccaaattt gccggggttg gtgcgggggg tcaaggaaat cggctgcgtc
     2941 gcccggaagg ttcggcgcag gggagcggct gcaccatcct tagatgcgac cgccccgtgc
     3001 gggactggtg ccgggtgagg gacttgaacc cccgaccttc ggtttacaaa accgctgcac
     3061 taccactgtg ctaacccggc gccggcggcg gccgtaggta tcagacttgg acggccactg
     3121 ccaggacata gacacgcgcc cgatgccctc gatgagtggc gctgcgttct agtcggctgg
     3181 ctcctgaatg gccgagggac gactacaaac gctctggtgc gaaccctgct tccgcacccg
     3241 tatgaatttt cttggcctgt gacctaaata acacaagttt aatcgctccg tccagcgaac
     3301 ggttagctgc gaagggccat gttcagcctg tcgccggtgc gaactgacca gaacaaccct
     3361 gcatcgaccc agtgccaagg cccaatttcg ggaacaggaa atgccaacat gaacatgaaa
     3421 ccccaacgcc gcaatgtgca taagatcgcg ttcgccgcga cgcttctcgc gggcaccagc
     3481 ctcggcggcc tcgcgcttag ccatggcgcg tgggcagacg atgccaccgc ggtcaacccg
     3541 ccgggcgcgc agctccagcc gcacaccctg ccggacttca ccaatctggt gacccaggta
     3601 aagcccgccg tcgtttccat caccaacaag atgaaagcca cgcccgtcgc cctggaacag
     3661 ggccaacagg gcggcgagat gcagcggctg ccgttcccgt tcaaccagat gattccgaac
     3721 gggccgcagc agcaagaccg agcggtcgag gcccgaggct ccggcttcat catcagcgcg
     3781 gacggcacca tcgtcaccaa caaccacgtc ataaagggtg cccgtacact cagcgtcacg
     3841 ctcgatgacg gcacggtgct gcccgcgaag gtggtcggca gcgatccccg caccgacatc
     3901 gccgtcctga aggtatccgc atcgcgtccg cttccgttca ttcaactcgg caactcgcgg
     3961 gacgtgaagc ccggcgagtg ggtcgtcgcg atgggcaacc cgttcggatt gggtggttcg
     4021 gtgaccgccg gcatcgtctc cgcggtcagc cgcgatatcg gtgccggccc gtacgaccag
     4081 ttcatccagg tcgatgcgcc gatcaaccaa ggcaattccg gtggtccgct gttcacccag
     4141 gacggcaagg tcatcggcat gaacacggcg atcctgtcgc ccaccggcgg gtcggtcggc
     4201 atcggtttcg ccgtcccatc cgacatgatc cgtacggtca cggcgcagct tgaaaagacc
     4261 ggcacggtgg ttcgcggtta tgtcggcgtg gaggcccagc cgatcacacc cagcaccgct
     4321 cgcgccctgc atctgaagga gaatgacggt gccttgctcg ccggcgttca gcccaacagc
     4381 ccggcagccg aagccggtct gcagccgggc gacgtgattc aggcggtcaa cggcgtcaag
     4441 gtcgccaacc caaaggaact ggcgctgaac gtcgccaaca tccagccgaa cgatcaggcg
     4501 catctaagcg tgctgcatga cggtcagacc cgggacgtca cggtcaaggt cggaacgctg
     4561 ccaaacgagc aaaccgccag caacgacaat ccggccggcg gcgagcgccg tgcccgcatc
     4621 ggcttggcgt tggcgccgtt gtcaccggat atgcgcgacc agttggacgt gccggagggc
     4681 accaaaggag cggtcgtgca gggggttcag cctggctcac ccgccgaggt ggccggtttg
     4741 caacccggcg acgttatcgt cggtgtgggc acgcgtgcgg taacctcccc ggcggaggcg
     4801 gctcgggcga tgcagacggc gttgaatggc aacgatcatg ccatggcgct gcgggtcatt
     4861 cgcaacgggc agccgatttt cgtcggcgta acggtggacg accagaacaa ccaaggctga
     4921 tgatgatcgc gccgggtgaa ccgccgacgg cgcgtgaatc atgtggtcaa cagatgccct
     4981 gaagcatgcc aatccctggc cggtgtagtt cccggccagg gagtggcgcg gccgatgggc
     5041 ttagggtcgg ctgccaaatc atggtgaatg aaggctacag cattcgatga cggtgtcatg
     5101 gccatgcttg gcgcggccag ccactatttg tcgttacagc gaaagtcgtg ggtcgccggc
     5161 aagagtccgg tcatgacaca agcggcaaac cgctatgact atcagtcaga cactcatggc
     5221 gtgatgccgg ccatgctatc cgaaaccgcg cttgaagcgt ctattttgaa gaagacagtt
     5281 aagccgatgc cggaacgcct gcaccgcccg cgggacgccc agtcttcgcg gcgtttagcg
     5341 gcacttcgac atcgatctcc aacgtcgaga acgatgctcc gcgatccagc ttgatctgaa
     5401 ccgcatcccg atcgaccgag atatgcttgg cgatcaccgc caaaatctcc tcctgcaatg
     5461 ttgccagcag gtcagacttg ccgatgatgc tgcgttcgtg cgccagcatc acctgcaggc
     5521 ggtcgcgtgc gaccggagcg gtggttttct tgcggaaaag ttcgaatagg ctcatgccac
     5581 tctccgtcca aacagccggc tgataaaccc gctgcgctgc atcggaatga ccacatcgag
     5641 cgtttcaccc ttcagtcttt tcgccgcatc gaagtaagcc ctggacggcg cgctgttcgg
     5701 attgttcaac gtcaccggcg acccgatatt cgacgccttg aggacttcct cgctttcagg
     5761 aatgattccg agcagtggga tcgacaggat ctcgagcacg tcctcgacct tcagcatttc
     5821 atttcgcgcc gctcggccag catcgtagcg tgtgaccaat agaaacttgt caatgcgttc
     5881 gccacgttcg gcgcgcagcg tcttcgagtc cagcatgccg atgatccggt cggaatcgcg
     5941 caccgacgac acttccgggt ttgtcaccac caccgccatt tcggcgttgc gcatcgccaa
     6001 cagcgcgcca cgctcgatac ccgccgggct gtcgcagatc acccagtcga acttttcctt
     6061 cagttcgccg atgaccttgt ccacgccttc catggtcagc gcgtccttgt cccgggtttg
     6121 cgacgccggc agcagcgaca gcgtgtcgat acgtttgtcg cgtatcaacg cctgcgccag
     6181 tttcgcgtcg ccctgcacga cgttgatcag gtcgaacacc acgcgccgtt ccgcgcccat
     6241 caccaggtcc agatttcgca atccgacgtc gaaatcgatg accacgacgt tctcaccgcc
     6301 acgcgcgagc gcagcaccga gcgcagcggt cgatgtggtc ttgccgaccc cacccttgcc
     6361 ggacgttacc accagcacct tgcccatctg cgtcccctta atccagaacc gatattttca
     6421 tttgttcacc atcaagcca
//
LOCUS       DASWQN010000261         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1742759, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000261 DASWQN010000000
VERSION     DASWQN010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742759"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VGC82_04310"
     CDS             complement(<1..302)
                     /locus_tag="VGC82_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422527.1"
                     /translation="MTAQPAKHVPIGAPMQHMLAYTIQSTPWANNRPSVIIDVGGKRS
                     VLVPANGQDNSYWIVILDAMDPRKVVKDWVIPGANQAVPAGIDTYLDDPKYIFAVCT"
     gene            complement(605..2092)
                     /locus_tag="VGC82_04315"
     CDS             complement(605..2092)
                     /locus_tag="VGC82_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0422528.1"
                     /translation="MPNIWTRKSTADLLLEAAPAAGGGETHSLKRSLSALDLVILGIG
                     GVIGAGIFILTGQAAATNAGPAVTLSFLLGAVACAFAGLCYAEMASAVPVSGSAYTYA
                     YATLGELVAWIIGWDLILEYAVAAVAVAVGWSAYVVSFARDFGIDLPSRLVSAPVAYD
                     SNTGAWSSTGALFNLPAMLVIVMVTALLVVGIRETARFNGFIVAVKLMVIVLFVGCAA
                     SAVTTANWVTSGNPDGAFIPPNAGTGAFGWSGVVRGAAMVFFAYVGFDAVSTAAQEAR
                     RPERDMPIGILGSLVVCAVLYIAVAFVLTGIVPFDRLNVADPIAVGIDAIGVGWLSPL
                     IKLGIVLGLTSVILVTLLGQPRIFRAMAHDGLLPPALARIHPRFRTPSVATIGGGAVV
                     AVLSGLLPIGLVGELVSIGTLFAFAVVSVGTLALRVIDPELPRPFRVPAIWAIAPAGA
                     ATSLFLMLGLPLDTWIRLGVWLLAGLAIYFAYGARHSRLRTADRR"
     gene            complement(2190..>2688)
                     /locus_tag="VGC82_04320"
     CDS             complement(2190..>2688)
                     /locus_tag="VGC82_04320"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY0422529.1"
                     /translation="LNRALKTTVRVCGNILFIVYAAYVFSYAISYAGVGEQITQFLVG
                     MKLSRLEFFFALFVLYTVLGCLVESLGMIVVTVPLLYPVLVQYGVDPIWFGVMLVLFI
                     EMGQISPPIGINLFVIQSIWTGKLSDVVAGTIPFHLIMFVLLGLLVLFPELAMWLPSR
                     AAAVP"
BASE COUNT          570 a          897 c          825 g          396 t
ORIGIN      
        1 gtgcagacgg cgaagatata tttcgggtcg tccagatagg tatcgatccc ggctggaacc
       61 gcctggttcg ccccggggat aacccaatcc ttgaccacct tgcgtggatc catcgcgtcc
      121 aggatgacaa tccagtaact gttgtcctgc ccgttggccg gcaccagaac gcttcgcttg
      181 cccccgacat cgatgatgac ggacgggcgg ttgttcgccc acggcgtact ctggatggtg
      241 taggccagca tgtgctgcat gggtgcaccg atcggcacgt gcttcgcagg ctgcgcggtc
      301 atggtctgct ccctccggtt tgatgcggcc cgggcatgct caacccaaga tgacccccga
      361 gcgatggcgc atactcatgg ttttcggtca ttcccgcaag aggtgtgccg caatcaatta
      421 caccatcaga tttcacaatc gcgttgtgcg gcttccgcca gccggcaccg gcaatggaag
      481 tcgccgatgg atcggatgcg cctgccacag cctccaaacc cgcatccgac cagaagattc
      541 agaagatatt tgattgactt agatcggcga cacctggatc agcctcctga tcaccgtcac
      601 cgccctaccg gcgatccgcc gtccgcaacc ggctgtgcct tgcgccatag gcgaaataga
      661 tcgcaagccc ggccagcagc cagacgccca gtctgatcca ggtatccaat ggcagcccca
      721 gcatcagaaa cagcgacgtc gccgcgccgg cgggagcgat cgcccaaatc gccggcaccc
      781 ggaacggccg cggcagttct ggatcgatca cgcgaagcgc aagcgttccg acggaaacga
      841 ccgcaaaggc aaacaacgtt ccgatgctga cgagttcgcc gacaagaccg atcggcagca
      901 ggccggacag caccgcgacg acggcgccgc cgccgatcgt tgccaccgac ggcgtccgga
      961 aacgcggatg gatccttgcc aatgccggcg gcagcaatcc gtcatgggcc atggcacgaa
     1021 aaatccgggg ctgcccgagc agcgtcacca ggatgaccga cgtcagcccg agcacgatcc
     1081 cgagcttgat cagcggcgag agccatccga cgccgatggc gtcgataccg accgcgatcg
     1141 gatctgcaac attcaaccgg tcgaacggca cgatcccggt gagcacgaag gccaccgcga
     1201 tatagagcac ggcgcaaacc acgagcgagc cgaggatgcc gatcggcatg tcgcgttccg
     1261 gcctcctcgc ttcctgcgcc gcggtcgaga ccgcgtcgaa accgacatag gcaaagaaca
     1321 ccatcgcagc cccccggacc acgccggacc agccgaacgc gccggtcccc gcgttcggcg
     1381 gaatgaaggc gccatccgga ttgcctgacg tcacccagtt cgctgtcgtc accgcggagg
     1441 cggcgcaacc cacgaagagg acgatcacca tcagtttcac ggcgacaatg aagccattga
     1501 agcgcgcggt ctcgcggatg ccgaccacca gcaaggccgt gaccatcacg atgacgagca
     1561 tggccggcaa gttgaacagc gcgccggtcg acgaccaggc gccggtattg gaatcatagg
     1621 caacgggtgc ggaaactagc cgcgacggca ggtcgatgcc gaaatccctg gcgaagctga
     1681 cgacataggc ggaccatccg accgccaccg cgaccgcggc gacggcgtat tcgaggatga
     1741 ggtcccagcc gatgatccag gcgaccagtt cgccgagcgt cgcataggca taggtgtacg
     1801 cactgccgct gaccggaacg gccgaggcca tttcggcgta acagaggccc gcgaacgcgc
     1861 aggccacggc gccgagcaga aacgacagcg ttaccgccgg cccggcattg gtcgccgcgg
     1921 cctgcccggt caggatgaag atgcccgccc cgatcacgcc gccgatgccg aggatcacga
     1981 gatcgagcgc cgacagcgaa cgcttcaggg agtgggtctc gccgccgccg gccgccggcg
     2041 cggcctccag caacagatcg gccgtggatt ttcgtgtcca gatgtttggc aacagcggct
     2101 cctcgcggcg cgggcctgcc gggcgccatc ggcgatgccg caatcctgac gccgtttgcc
     2161 tgagcccgca accggccgcg tgccggttct cacggcaccg ctgccgccct gcttggcagc
     2221 cacatcgcga gttccgggaa cagcaccagc agtcccagca gcacgaacat gatcaggtgg
     2281 aacgggatcg tgccggcgac gacatcactg agcttgccgg tccagatgct ctggatcacg
     2341 aacagattga tcccgatcgg cggcgaaatc tggcccatct cgatgaacag caccagcatg
     2401 acgccgaacc agatcggatc gacgccgtat tgcaccagca cgggatagag cagcggcacg
     2461 gtgacgacga tcatgccgag gctttcgacc aaacatccca gcacggtgta gagcacgaac
     2521 agggcgaaga agaactccag ccgcgacagc ttcatgccga ccaggaactg ggtgatctgc
     2581 tcgccgacgc cggcgtagct gatggcgtag gaaaagacgt aggccgcgta gacgatgaag
     2641 aggatgttgc cgcacacccg caccgtcgtc ttcagcgccc gattgagc
//
LOCUS       DASWQN010000262         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1874986, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000262 DASWQN010000000
VERSION     DASWQN010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874986"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VGC82_04325"
     CDS             <1..846
                     /locus_tag="VGC82_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY0422530.1"
                     /translation="VVRPNVAMDAAVKELTAAAERIAKAGGTPLAVAKDGKLLGVITL
                     KDIVKGGIRERFSELRKMGIRTVMITGDNPLTAAAIAAEAGVDDFLAQATPEAKLKLI
                     RDEQAAGKLVAMCGDGTNDAPALAQADVGVAMNTGTMAAREAGNMVDLDSDPTKLIEI
                     VEIGKQLLMTRGALTTFSIANDVAKYFAMIPAMFIAFYPQLQALNIMRLGNPQSAILS
                     AIIFNALIIVALIPLALRGIAYRPIGAAALLRRNLLIYGLGGIAAPFVGIKLIDLAVT
                     ALGLA"
     gene            1009..1614
                     /locus_tag="VGC82_04330"
     CDS             1009..1614
                     /locus_tag="VGC82_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HEY0422531.1"
                     /translation="MLQHFRPAVMMIVCMTILTGLAYPLAMTGVAQAIFPRQANGSLI
                     ERDGRTIGSALIGQNFTAANYFHGRPSATTAPDPNDDTKTVPAPYNAGNSGGSNLGPT
                     SKALVDRMTADTAALAAENPGTPVPMDLVTTSASGLDPDISPAAALFQIPRVAKARNL
                     PAAKIQELVEDHVVGRVAGIIGEPRVNVLQLNLALDALGRG"
     gene            1614..>2484
                     /locus_tag="VGC82_04335"
     CDS             1614..>2484
                     /locus_tag="VGC82_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEY0422532.1"
                     /translation="MDESTRIDRRPLPEALLREAEKASRGRLKIFLGAAPGVGKTYEM
                     LSAAQARRRENVDVVIGVVETHGRAETEALTQGLETIPRRTIAYRERTLTEMDLDAIL
                     ARRPSLVLVDELAHTNAPGSRHPKRYLDVEELLTAGIDVYTTLNIQHVESLNDVVARI
                     TRIRVRETVPDSVIERADDIELIDLTPDDLIQRLKDGKVYVPAQAERAARHYFAPGNL
                     TALRELALRRTAQRVDNQMVDYMRSHAIEGPWQAGERVLVCIQGGSRASATVRHAKRL
                     ADQLRAPWTALNVE"
BASE COUNT          500 a          776 c          769 g          439 t
ORIGIN      
        1 gtggttcgac ccaacgtggc aatggatgcc gccgtcaagg aactgaccgc ggcggctgaa
       61 aggatcgcca aggccggcgg cacgccgctg gccgtggcaa aggacggcaa gttgctgggg
      121 gtgattaccc tcaaggatat cgtcaagggc ggcatccgcg agcgcttcag cgaactgcgg
      181 aaaatgggca tccgcaccgt gatgatcacc ggcgacaacc cgctgaccgc cgctgccatc
      241 gccgccgagg cgggggttga cgacttcctg gcgcaggcaa cgccggaagc gaagttgaag
      301 ctgatccgcg acgagcaggc cgccggcaaa ctggttgcca tgtgcggcga cggcaccaac
      361 gatgctcctg ccctggctca ggccgatgtc ggcgtggcga tgaataccgg caccatggcc
      421 gcgcgcgagg ccggcaacat ggtcgatctc gacagcgacc cgaccaagct gatcgagatc
      481 gtcgagatcg gcaagcagtt gctgatgacc cgcggcgccc tgacgacgtt ttctatcgcc
      541 aatgatgtcg caaagtactt cgcaatgatc ccggcgatgt tcatcgcatt ctatccgcaa
      601 ctgcaggccc tcaatatcat gcggctgggc aatccgcaga gtgcgatcct gtcggcgatc
      661 atcttcaacg cgctgatcat cgtggcactg ataccgctgg cgctgcgcgg gattgcgtat
      721 cgtcccatcg gtgccgccgc gctgttgcgg cgcaacctgc tgatctacgg gctgggcggg
      781 attgcagcac cttttgtcgg gatcaagctg atcgacctcg cggtcaccgc gctcggtctg
      841 gcttaacgtg gaagatcacg cgaatcatgc aaagaaaaga tgatgactca tatacaaaac
      901 aaaaggttcg agaccagtat cgttgcttgg gccttccatg acgattgctt ggacgttcga
      961 ttggtttgat gggtttattt cgtcatcaca tgtctaggac aatgaacgat gctacagcat
     1021 ttccgacccg ccgtcatgat gatcgtctgt atgacgatcc tgactggcct tgcctatccg
     1081 ctcgcgatga ccggcgtcgc gcaggccatc tttccccgac aggccaacgg cagcctgatc
     1141 gagcgcgacg gcaggaccat cggctccgcc ctcatcggcc agaacttcac ggcggcaaac
     1201 tacttccatg gccggccgtc cgcgaccacc gccccggacc ctaacgacga tacgaagact
     1261 gtcccggcgc cgtacaatgc cggcaactcc ggcgggtcca accttgggcc gacctcgaaa
     1321 gccctggtgg atcggatgac ggcggacacc gcggccctgg ccgccgagaa ccctggcacg
     1381 ccggtgccga tggatctggt gacgacctcc gccagcggac tggaccccga tatctcgcca
     1441 gccgccgctc tgttccagat tccccgcgtt gccaaggcgc gtaacctgcc ggcagcgaaa
     1501 attcaggaat tggtggagga tcacgttgtt ggccgggtcg ccggtatcat cggcgaacca
     1561 cgtgtgaacg tgctacagtt gaatttggca ttggatgcac ttggccgagg ctaatggacg
     1621 aaagcacgcg catcgatcgg cggccattac cggaagcatt gctgcgggag gcggagaaag
     1681 cttcccgcgg ccgcttgaag attttcctcg gcgccgcgcc gggggtcggt aaaacctatg
     1741 agatgctgtc ggccgcgcaa gccaggcgcc gggagaacgt ggacgtcgtc atcggcgtgg
     1801 tcgaaaccca tggcagggca gaaaccgaag cgctgaccca aggcctggag accatcccgc
     1861 gccggaccat cgcctaccgc gagcggactc tgactgaaat ggacctcgac gcgatcctcg
     1921 ctcgccgtcc atccctggtt ttggtcgatg aactcgccca tacaaacgcg cccggcagcc
     1981 ggcatcccaa acgatacctg gatgtcgagg aactgctgac cgccggcatc gacgtttaca
     2041 ccacgctgaa catccagcat gtcgaaagcc tgaacgatgt ggtggctcgc atcacccgca
     2101 tccgggttcg cgagacggtg ccggattcgg tgatcgaacg agccgacgac atcgaactga
     2161 tcgatctcac gccggacgac ctgatccagc gcctgaagga cggcaaggtc tatgtcccgg
     2221 cccaggccga acgcgcggcc cggcattatt ttgcgcccgg caacctgact gcattgcggg
     2281 aattggcgtt gcgacgaacc gctcagcgtg tggacaacca gatggttgac tacatgcgca
     2341 gtcacgctat cgaagggcca tggcaggccg gagagcgcgt gctggtttgc atccagggcg
     2401 gatccagggc gtcggctacg gtgcgccatg ccaagcggct ggcggaccag ctccgcgcgc
     2461 catggaccgc gctgaatgtc gaaa
//
LOCUS       DASWQN010000263         3141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2270742, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000263 DASWQN010000000
VERSION     DASWQN010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2270742"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1114)
                     /locus_tag="VGC82_04340"
     CDS             complement(17..1114)
                     /locus_tag="VGC82_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0422533.1"
                     /translation="MNRRAFLATSTVGATASVALPFAAAMEVPLLGETGNPLLVPAGE
                     RLPLGPLSGSRYPDPHIEALDNKRFKGSPGTGAVERVATGFRWAEGPAYFRAGRYLIF
                     SDIPNNRQMRLLEDDNHLSVFRSPSYNSNGNTVDRQGRLITCQHAGRRVVRTELDGRI
                     TIIADSYNGKKLNSPNDVVVASDDSVWFTDPSYGIGGNYEGLQAAQEQATHNVYRVDA
                     RSGEVKMLVDDFTQPNGLAFSPDEKKLYIVDSGITHGGPAHIRVFDVDVGAGKLTNSK
                     VFAEGFAPGFTDGLRLDVEGNVWASMGWADPKEDGVRCYAPNGDLIGKIHLPETCANL
                     TFGGLLRNRLYMCASTSIYALYVNTQGAAVP"
     gene            complement(1358..2218)
                     /gene="uraD"
                     /locus_tag="VGC82_04345"
     CDS             complement(1358..2218)
                     /gene="uraD"
                     /locus_tag="VGC82_04345"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEY0422534.1"
                     /translation="MHTLDQLNVMSPGGFAAALDGVFEHASWVAEAAASQRPFPTVSA
                     LHDALMAVVRTAPADIRIRFLQGHPALSPKALADPGLTASSRTEQSDLGMSSLGEHLG
                     KFEESSAAYEQRFGFPFIVCVRRQTPPFVLRNLERRLGHDLDQELAAALTEIGYIARL
                     RLVERVTGPGMPKTAGHLSTHVLDTARGKPAEGVRVELFREDVMITTSLTNHDGRTEA
                     PLLPDGPLRVGRYELRFHVEDYFAGWPNVPSPPWYDVIPIRFGIAEPEGHYHVPLLLG
                     AWTYTTYRGS"
     gene            complement(2249..3079)
                     /locus_tag="VGC82_04350"
     CDS             complement(2249..3079)
                     /locus_tag="VGC82_04350"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0422535.1"
                     /translation="MSETLSPVRQRQGLATASFRSAAGGLAAAALFLLAAPTLIVVIV
                     SFTSGLSLRFPPPGLSLRWYESLADSWQLQFAARNSLMVGIAATGMAVLLGVPAALAI
                     GRSRSGMARALDQLFLSPLILPGLAFGLASLIFFTSIGLTVSVTTLVLGHTIVSVPYV
                     VRNTVAALAQLDPAQIDSSLSLGASRLMTFRRVTLPSIRSGILSGAFVCFMASFDNIP
                     VSLFLRDAATDMLPIRMWQDLEGRLDVTIAAASSALIILTIILAVVMERLVGLSRRLQ
                     "
BASE COUNT          625 a          967 c          993 g          556 t
ORIGIN      
        1 actcggggcc aagccgtcaa ggaaccgccg caccttgggt gttgacgtat aacgcataga
       61 tggacgtgct ggcgcacatg tacagcctgt ttcgcaacag cccgccgaac gtcaggttgg
      121 cgcacgtttc cggcaggtgg atcttgccga tcaggtcgcc gttcggcgcg tagcagcgaa
      181 cgccatcctc cttcggatcg gcccagccca tgcttgccca gacattgccc tcgacgtcga
      241 ggcgcagccc gtcggtaaag ccgggcgcga agccttcggc gaagaccttg ctgttggtca
      301 acttgccagc tccgacatcg acatcgaaca cccggatatg cgccggtccg ccgtgcgtga
      361 tgccgctatc gacgatgtag agtttctttt catccggcga gaacgccagg ccgttcggtt
      421 gggtaaagtc gtcgaccagc atcttgacct cgccgctacg cgcatccaca cggtagacat
      481 tgtgggtggc ctgttcctgc gccgcttgca ggccctcgta gttgccgccg atgccatacg
      541 acggatcggt gaaccagacc gaatcatcgg aagccacgac gacatcgttt ggtgaattga
      601 gcttcttgcc gttgtagctg tcggcgatga tggtgatgcg tccgtcaagt tcggtacgca
      661 ccacccgccg cccggcatgc tggcaggtga tcaggcgccc ctggcgatcc acggtgttgc
      721 cattcgagtt atacgatggc gagcggaaga cgctgaggtg gttgtcatcc tccagcagcc
      781 gcatttgccg attgttcggg atgtcgctga aaataaggta gcggcccgcg cggaaatacg
      841 ccggcccttc ggcccatcga aagccggtcg cgacacgctc taccgcaccg gtgccgggac
      901 tgcctttgaa gcgcttgttg tccagcgcct cgatgtgcgg atccggatag cgggagccgg
      961 acaatggacc caacggcagt cgctcgccgg cgggaaccag cagcggattg cccgtttcgc
     1021 ccaacaatgg cacctccatg gcggcagcaa atggtagtgc gacggaagct gttgcgccga
     1081 ccgtggatgt ggccaggaat gcgcgtctgt tcattgcttc ttcgtctccc ttttgctgga
     1141 gcgtttcggc ctgactgtac aggctgaaaa tactctagct ctttgtctgc tcgtatgatt
     1201 cacacgcttc ggacgttccg tccagcatga ccatgctcta gctatttgtc tgctcgcatg
     1261 attcacccgc ttcggacgtt ccgcccagcg tgatcatgct ccttgtctgc tcgcatgatt
     1321 cacccgcttc ggacgtttcg cccagcgtga tcatgctcta actcccgcga taggtcgtat
     1381 aagtccaggc accgagcaac agcggcacat gataatggcc ttcgggttcg gcgatgccga
     1441 agcgaatcgg gatcacatca taccaaggtg ggctggggac gttcggccaa ccggcgaagt
     1501 agtcctcgac atggaaccgc agctcgtagc gcccgacgcg caacgggcca tcaggcagca
     1561 acggagcctc ggttcgtcca tcgtggttgg tgaggcttgt ggtgatcatg acgtcttcgc
     1621 gaaacaattc cacccgcacg ccctcggcgg gctttccacg agcggtatcc aggacgtggg
     1681 tcgatagatg tcccgcggtt ttcggcatgc cggggcccgt gacacgctcc accaggcgca
     1741 gacgcgcgat gtagccaatc tcggtcaagg cggcggccag ttcctggtcc aggtcgtgac
     1801 cgaggcggcg ttcaaggttg cgcagcacga agggcggcgt ctggcggcgc acacaaacga
     1861 tgaacgggaa gccgaatcgc tgctcgtaag cggccgagga ctcttcgaat ttccccaggt
     1921 gctcgcccag gctgctcatg ccgaggtcac tctgttcggt gcgggaggac gctgtcaggc
     1981 caggatcggc cagggctttc ggcgacaacg ccgggtggcc ttgaagaaag cggatacgga
     2041 tatcggccgg ggcagtgcgg accaccgcca tcagggcgtc gtgcaaggcg gaaaccgtcg
     2101 gaaacgggcg ttgcgatgcg gcggcctcgg cgacccagga tgcatgctcg aacaccccgt
     2161 ccagggcagc ggcgaaaccg cccggcgaca tgacgttgag ttggtcgagc gtatgcatcg
     2221 gcattccttt actacaggcg acattccttt actgcaggcg gcgcgacaag ccgacgagtc
     2281 gctccatcac cacggccaga atgatcgtaa ggatgatcaa cgcagacgat gcggcagcga
     2341 tggtgacgtc caaccgcccc tccaggtcct gccacatgcg gatcggcagc atgtcggtcg
     2401 cggcatcgcg caggaacagc gacaccggga tgttgtcgaa cgacgccatg aagcagacga
     2461 aggcgccgga caggatgccg gaccggatcg agggcagcgt gacccgccgg aacgtcatca
     2521 accgggacgc gccgagactg agggaactgt cgatctgcgc cgggtccagt tgtgcgagcg
     2581 cagcgactgt gttgcgcacg acataaggca cgctgacgat ggtatgacca agcaccaacg
     2641 tcgtcaccga aactgtgagt ccaatcgagg taaagaagat caggctggcc agcccgaatg
     2701 ccaggccggg caagatcagg ggcgagagga aaagctgatc cagtgcccgc gccatgccgg
     2761 accggctgcg tccgatcgcc agcgccgccg gaacgccaag aagcacagcc atgccagtcg
     2821 cggcgatgcc taccatcagc gagttccggg ctgcgaactg caactgccat gagtccgcga
     2881 gcgactcgta ccaccgcaac gacagcccgg gcggcggaaa gcgcaacgac aagccactgg
     2941 taaatgaaac gatcacgacg atcagcgtcg gtgcggccaa caggaacaac gctgcagcgg
     3001 cgagtcctcc ggcggcggat cggaaggatg cggtggccag gccttggcgc tgacggacag
     3061 gagataaggt ctcagacatt cgccgctccc ctggacaagc gggacagcgc cgtcagcacc
     3121 gcaaggacag cgagcaccgc t
//
LOCUS       DASWQN010000264         5514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2139046, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000264 DASWQN010000000
VERSION     DASWQN010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139046"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..604
                     /locus_tag="VGC82_04355"
     CDS             26..604
                     /locus_tag="VGC82_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0422536.1"
                     /translation="MLKEANSESIGRGATFAQRCTMCHGARGLSDANSPNLAGQYPAV
                     IYKQLLDFKSGARTSVVMAPLVTDLNEQDMRELAAYYAYLPRLPAFHPPVAGPAPLIV
                     LDGAPMRNIPPCGSCHGGLANKTGSAWLEGQPEACLRTQLLAFASGTRHNDISQQMRN
                     VARNMTPTEIDEAARYYASQPGAGAGESKPIP"
     gene            759..2567
                     /locus_tag="VGC82_04360"
     CDS             759..2567
                     /locus_tag="VGC82_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0422537.1"
                     /translation="MAPPLLTLQDIALTFGAAPLLAGAGISVSTGDRVCLVGRNGSGK
                     STLLRIAAGLIQADSGRRFAQPGATIRYLPQEPDFAGYATTLDYVEAGLNHASASDHY
                     RASYLLEQLGLTGTEDPMKLSGGESRRTALARVLAPEPDILLLDEPTNHLDLPGIEWL
                     EAELANMRSGIVLISHDRRLLERISRGTVWLDRGITRVLDQGFAQFEAWRDQVLEQEE
                     TDRHKTARKIVMEEDWLRYGVTARRKRNQKRLGDLHALRKKHKEQRGALGKVKLEAAE
                     ANLSGRLVAVAEDVSKAYGDRVVVRDFSTRIIRGDRVGIVGRNGAGKTTLLNLLTGKQ
                     DPDTGQVKLGTNLAEVALDQRRESLDPAQTLSQVLTGGAGDTVVVGGQTRHVVSYMKD
                     FLFGPEQANTPVGVLSGGERGRLTLARAFATPSNLMVLDEPTNDLDLETLELLQERLA
                     DYAGTVLLVSHDRDFLDRVVTSVIATEGDGRWIEYAGGYSDMLLQRGPARTTSGMSAG
                     LPRGSRDDAPARPQSRKMSFKDKHALETLPVRMAKLETEVARLNRVLADPDLFTRQPA
                     KFAAATKALEATQASLTEAEEQWLALEMQREDLERA"
     gene            complement(2913..3212)
                     /locus_tag="VGC82_04365"
     CDS             complement(2913..3212)
                     /locus_tag="VGC82_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422538.1"
                     /translation="MNAALKPAATLEAFLDWESRQETKCEFDGFQPVAMTGGTAAHAA
                     IQRNLIGLLYNRLRGHRYQAFDSELNSGWARHSFIPMPSSCVRRCLPTVWSSWNL"
     gene            3528..4502
                     /locus_tag="VGC82_04370"
     CDS             3528..4502
                     /locus_tag="VGC82_04370"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY0422539.1"
                     /translation="MSDSIVGIDVSKETLDVNCATGQRKQARIFANHPEGWKLVLSWL
                     KAMGNKQAHVCMEATGRHSLGVALALHDAGHVVSVVNPAQIRDFARTKLGRNKTDKVD
                     AVLIRDYAALFNPAPWTPPSPAMRQLCELQTVRAGIIGSRTEWKNRLASGLGDSTATQ
                     LAAATIEHFTHQLEAVDRAIGETIDQDATLRGRRDLLLSINGVGGTLAASLLAEMPEP
                     GVLRRSGELVAYAGLNPSHHRSGTSIDRPTRISKIGNATLRSSLYMPALSAMRFNPAV
                     AALVGRLKEAGRLTPKQIVVAAMRKLLVLCFGVLKTGKPFDPAIAMPA"
     gene            complement(4854..5073)
                     /locus_tag="VGC82_04375"
                     /pseudo
     CDS             complement(4854..5073)
                     /locus_tag="VGC82_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007792467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5153..5332
                     /locus_tag="VGC82_04380"
     CDS             5153..5332
                     /locus_tag="VGC82_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422540.1"
                     /translation="MPRIALGYTKRNLAVAETGEFAASLDMEAFNQARCSQTSDHREA
                     MQSFKEKRRPVFDGR"
BASE COUNT         1069 a         1736 c         1724 g          985 t
ORIGIN      
        1 cagcggtgtg gtggtaacac cacagatgct gaaggaggcg aactcggaat cgattggtcg
       61 aggtgccacg ttcgcgcagc gttgcacgat gtgccacggc gcaagggggc tgagcgacgc
      121 caattcgccg aatctggcgg gtcaatatcc cgccgtgatt tacaaacaac tgctcgattt
      181 caaatcgggt gcgcgcacga gtgtcgtcat ggcaccgctg gtcacggatc tcaatgagca
      241 ggacatgcgt gaattggcgg cgtactatgc ctacttgccg cgtctgccag ctttccatcc
      301 accggtggcc ggaccggctc cgctcatcgt gctcgacggt gcgccgatgc gtaacatccc
      361 gccctgcgga tcgtgccatg gcggcttggc caacaaaacc ggcagcgcct ggctggaagg
      421 tcagccggag gcctgtctcc gaacgcagct tctggcattc gcatccggca cccgccacaa
      481 cgacatcagc cagcagatgc ggaatgtggc gcgtaacatg acgccgaccg aaatagatga
      541 agccgcacgt tactatgcga gccaacctgg agccggcgca ggggaaagca aaccgattcc
      601 gtagcgtctg cgtgccgaaa tcgttcgagc agcactgcct tgactgatag gcgcgtccgg
      661 tcagcggagc agccatatcg atgtgcgact ctcgatgagt acgaccctcg atttgtacga
      721 ctgggcgata tagctctgaa gccctatata gcgtcgccat ggcccctccc ctactcacgc
      781 ttcaggatat cgccctgact ttcggcgccg cgccgctact ggcaggcgcg ggcatttcag
      841 tttccaccgg tgaccgtgtc tgcctcgtgg gacgcaacgg ctccggcaaa tccactctgt
      901 tgcgaatcgc cgccggcctg atccaggcgg actccggccg ccggttcgcg caacccggcg
      961 ccacgatccg gtatctgccg caagagccag atttcgccgg ctacgcgacc accctggatt
     1021 acgtggaagc cggattgaac cacgcctcgg cctccgacca ttaccgagcc tcttacctgc
     1081 tggaacaact cggcctgacc ggcaccgaag acccgatgaa actgtctggt ggtgaaagcc
     1141 gccgcaccgc cctggcgcgt gtcctcgcgc cggagcccga catcctgcta ttggacgaac
     1201 cgaccaacca cctcgacctt cctggcatcg agtggctgga ggccgaattg gccaacatgc
     1261 gctccggcat cgtgctgatc agccatgacc gaaggctgct ggagcggatc tcgcgcggca
     1321 ccgtctggct ggatcgcggc atcacccgtg tcctggatca gggcttcgcc cagttcgagg
     1381 cgtggcgcga ccaggtgctg gagcaggagg aaaccgaccg gcacaagacg gcccgcaaga
     1441 tcgtcatgga ggaggactgg ctgcgctacg gcgtcaccgc ccggcgcaaa cgcaaccaga
     1501 agcgcctcgg cgacctgcac gcgctacgca agaagcacaa ggaacagcgc ggcgcactcg
     1561 gtaaagtcaa gctggaagca gcggaggcaa acctgtccgg ccgcttggtc gccgtggcgg
     1621 aggacgtgtc gaaagcctac ggcgaccggg tcgtggtgcg ggatttctcc acgcgaatca
     1681 tccgaggcga ccgggttggc atcgtcggcc gcaacggcgc ggggaaaacc acgctgctca
     1741 atctgctgac cggcaaacag gatcccgaca ccgggcaggt aaaactcggc accaacctgg
     1801 ccgaggtcgc gctcgaccag cggcgggaaa gcctcgatcc tgcgcaaacc ctgtcgcagg
     1861 tgctgaccgg aggggcgggc gataccgtcg tggtcggcgg gcagacgcga catgtggtca
     1921 gctacatgaa ggacttcctg ttcggcccgg agcaggcgaa cacgccggtc ggcgtgctgt
     1981 cgggcggcga gcgcgggcgg ctgactttgg cgcgggcctt cgccacgcca tccaacctga
     2041 tggtgctgga cgaaccgacc aacgaccttg acctggagac gctggagctg ctgcaggagc
     2101 ggctggcgga ttacgccggc accgtgctgc tggtcagcca cgaccgcgac ttcctggacc
     2161 gcgtggtgac gtcggtgatc gcgaccgaag gcgacgggcg ctggatcgaa tacgccggcg
     2221 gttactccga catgctgctg cagcgagggc cggccaggac gacgtccggc atgtcggcgg
     2281 gactgccgcg cggatcgcgc gacgacgcgc ccgccaggcc gcagagtcgg aaaatgagtt
     2341 tcaaggacaa gcacgcgttg gagacgctgc cggttcggat ggcgaagttg gaaaccgaag
     2401 tcgctcggct caatcgtgta ttggccgatc ccgatctctt tacacggcag ccggcgaaat
     2461 tcgctgccgc gaccaaggcg ctggaagcca cgcaagcgtc gttgaccgag gcggaagagc
     2521 aatggctggc tctggaaatg cagcgggagg acctcgagag ggcttgagcc gttcccgccg
     2581 ccgaggtgcc tccataaggc cgaagcaggg catggaacct tttactccaa accctcttgc
     2641 ggcccgccct gatcggactc ccggactggg aacgccacac ctttataaat atccaacagc
     2701 ggcagttcgg ttttacttcc ggcggatcca gaatggtgtc gctgatctgc tggtgcccaa
     2761 tccaatcctc gccgtcgcgg cggaagacgg ttgccgccgc cttggtttgg tcaaggatcg
     2821 cgtagcgctg gatggacggc atgtcccgat attcgtggtt ctgtcgatac gatcggtcgt
     2881 ggaggtattt tcgctgagga tttcgaaaac gatcacaggt tccaggacga ccagactgtt
     2941 ggcaggcacc ggcgaacaca cgatgaaggc atcgggataa acgaatggcg cgcccagcct
     3001 gaattcagtt cgctgtcaaa ggcttgatag cggtggccgc gcaggcggtt gtacagcagt
     3061 ccgatcagat tgcgctggat cgccgcgtgg gcggcagttc caccggtcat cgcaaccggc
     3121 tggaacccgt cgaattcgca cttggtttct tgccggcttt cccagtcgag gaatgcctcc
     3181 aacgtcgctg ccggtttcag cgccgcgttc atggcgcatg cttaacacgg atgcaccaca
     3241 cgggctacgc gcgtgccatc tatcggaaat cgccgacgct actgcagtcc cttgtacgtt
     3301 gcggcttgct cggcgggcga caatatctcg ctgaagcagc aaggaaagcg gtgagcctcg
     3361 tgcgtccctt cggtccacaa acctgtgcgg tagatatggc cccgcttctc cgagctccgc
     3421 tgttcatgcc ggacggtatc cagggacgcg gcatgccgct gatcccgcgt acaaggtggc
     3481 ccgacgcgga gccttgccgt gttcatcccc ttaaatggta gcaagacgtg agtgactcta
     3541 tagtcggaat cgatgtgtcc aaggaaacct tggacgtgaa ctgtgcgaca ggtcagcgaa
     3601 agcaagccag gatcttcgcg aatcatcccg agggctggaa gctggtactg tcctggctga
     3661 aggccatggg caacaaacaa gctcatgtct gcatggaagc gaccgggcgt cacagccttg
     3721 gcgttgcact ggcgttgcac gacgcaggtc atgtcgtcag cgtcgtcaac ccggcccaga
     3781 tccgcgactt cgcccgcacc aagctcggtc gcaataagac cgacaaggtc gatgccgtcc
     3841 tgattcgcga ctacgccgcg ttgttcaacc cggcgccatg gacgccgcca tctcctgcaa
     3901 tgcgacaact gtgcgaactg cagacagtcc gggccggcat cattggtagc cggacggagt
     3961 ggaagaatcg cctcgccagt ggtcttggcg acagcaccgc gacccaattg gcagccgcga
     4021 cgatcgagca tttcacgcac caacttgagg cggtcgaccg ggctattggt gagacgatcg
     4081 accaagacgc cacgttgcgc ggtagacgcg atctgctgct cagcatcaat ggcgtcggtg
     4141 gaacgctggc cgcgtcgctc ctggcggaaa tgccggagcc cggagttctg cgccgaagtg
     4201 gtgagttggt cgcttatgcg gggctcaatc cgagccacca ccgatccggc acgtccatcg
     4261 accggccaac gcgcatttcc aagatcggca atgccacctt gcgatcttcg ctctacatgc
     4321 cagcgctgtc ggcgatgcgt ttcaatcccg ccgtcgcagc gcttgtcggc cgcctcaagg
     4381 aagcgggccg ccttacgccc aagcagatcg ttgttgctgc catgcgcaag ctcctggtcc
     4441 tctgtttcgg tgtgctgaag acgggcaagc cgttcgaccc ggccatcgcc atgcctgcct
     4501 gagccttgct gacttccgct attccgttgt tcgattgcaa accaatcatt ccgccgtccc
     4561 gatctgtcag caggtcagtc ggtgtgcgca gcggaggtca aggagggccg caggcccgcg
     4621 cacggcagtg cgccttgtcc ttgaccgtag cgagcacgct gacacgctct gccaaatctc
     4681 ggggaccgcc aaaatccgca tccagcgctt gacaggcaac acggtatcta ccgggatggg
     4741 aaaggccaaa tcgccctcgt caccaaatcc agaggaagca cattagcgca tgcgtcaggt
     4801 gtcaggctaa ttgccgcact cgggctgccg ctggcaggta tccatcatgc caatcggtgc
     4861 cacgtggcat gcagcacctt ggcaacgcca gctttcggtc gctcgtgcgc atcgaagatc
     4921 gctgggttaa cgccgcgcag gccgcgccgg ggcagggagc gaaggaactc ggtggagaag
     4981 gtttctgcct cggaggcgcc gaccgtcata ccgacatttc acggcggccg cgtcgttgac
     5041 gcccacggcg atagtcaccg cgaccgagac aatgtgcgcg gcaggacgat cggctcgttt
     5101 ttggtgaata taacagcttc atcaagatca gctggcgcac aggatcgcct cgatgccgcg
     5161 catcgccctg ggctacacga aaaggaatct cgctgtcgcg gaaacgggcg agttcgctgc
     5221 ctccctggac atggaggcgt tcaaccaggc ccgctgttcc caaacctcgg atcaccgcga
     5281 ggccatgcag tcgttcaagg agaagcgcag gcccgtgttc gacgggcgct gatcagaggc
     5341 agaacggtta ccttctggct cgtctggcaa acgatcccgc ggtctttcca gccgattgcc
     5401 ggtccccgac cctcggaaaa ctcggcgctt cccagtcgcg gatccgtcgg catgatgacg
     5461 ccagcgcgcg cgcccttcag cccgtgtatc aaggtcggcg ctttgatgaa cttg
//
LOCUS       DASWQN010000265         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_819788, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000265 DASWQN010000000
VERSION     DASWQN010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819788"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VGC82_04385"
     CDS             <1..267
                     /locus_tag="VGC82_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422541.1"
                     /translation="GVDLRAVTIWALYGAVDWNSLLTKRDGVYERGAFDARHPDGTPR
                     PTLIAEAAASLARHGTVDHLLPDEPGWWRRDDRFHVPLVRLAAE"
     gene            377..>1533
                     /locus_tag="VGC82_04390"
     CDS             377..>1533
                     /locus_tag="VGC82_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HEY0422542.1"
                     /translation="MRYQPRLFRSFFLGGFECSTHRRADGRRLDLIAATHHDTLAEHD
                     YAQLRENGIQSARDGVRWHLIETRPGRYDWSSLLPLLRAAETQNVQVIWDLCHYGWPD
                     WLDVWSAAFPRRFARFAAAFAALHMQETGRPPQVCPVNEISFLSWAGGEFGMMNPHCN
                     DRSHELKRQLVRSMLEAGAAIRSAAPGAQIFAIDPLINVVPRQGTDPVTAVRNTECQY
                     QAFDMLLGQQEPELGGGPDAVDVLGVNFYWNNQWWYPDLPAPWGREALSVFDPHARPF
                     SDMLAEAYHRYGCPIFVAETSIEGRSRSAWLRYVCSEVYEAIEQGVPVEGICLYPVMS
                     HPGWDDGRYCSNGLFEMAWRSGRREVCTPLAEELRSQQQITSGLLGSGANRA"
BASE COUNT          258 a          469 c          514 g          292 t
ORIGIN      
        1 ggagtggatt tgcgcgcggt caccatttgg gcgctttacg gcgcggttga ctggaacagc
       61 ctgctgacga agcgggacgg cgtgtacgag cggggtgcct ttgacgctcg gcatccggac
      121 ggcacgccac gaccgacttt aatcgccgag gctgccgcgt cgctggcgcg ccatgggacc
      181 gttgatcatt tacttccgga cgaaccgggc tggtggcgcc gcgacgaccg gtttcatgtg
      241 ccgctggtca ggcttgccgc ggagtgagga tgatcggctt cgaggtcggc cggatgttca
      301 tcggatactg cccaatgttt cgcagcaaac gcgcggcgcg ggtcgatagt aagcggcgcg
      361 tgcgctggag taggacgtga gataccagcc aagactcttt cgcagcttct ttctgggagg
      421 gttcgagtgc tcgacgcacc gccgcgcgga cggacggcgg ctcgacctta tcgcggcaac
      481 ccatcacgac accttggcgg agcacgatta tgctcagttg cgtgagaatg gcatccagag
      541 tgcgcgcgac ggcgttcgct ggcacctgat cgagaccagg ccgggccgct atgactggtc
      601 atcgctattg ccgctgttgc gtgccgcgga aacgcagaac gtccaggtta tctgggatct
      661 ttgccactac ggctggcccg actggctcga cgtctggtcg gctgctttcc cgcgccgctt
      721 cgcccgcttc gccgccgcgt tcgccgcgct gcacatgcag gaaaccgggc gtccgccaca
      781 ggtttgtccg gtcaatgaga tctccttcct gtcatgggcc ggcggcgaat tcggcatgat
      841 gaacccgcat tgcaacgacc gcagccatga actgaagcgt cagttggtgc gcagcatgct
      901 ggaggccggg gcagccattc gatccgccgc accgggcgcg cagatcttcg ccatcgatcc
      961 gctgatcaac gtcgtgccgc gtcagggcac cgatcctgtc acggccgtgc ggaacacgga
     1021 atgccagtac caggccttcg atatgctgct ggggcagcag gaacccgaac tcggtggcgg
     1081 tcccgacgcg gtggacgtgc tgggggtcaa cttctactgg aacaaccagt ggtggtaccc
     1141 cgacctgccg gcgccctggg gccgggaggc gctatcggtc ttcgacccgc acgcacgccc
     1201 gttcagcgac atgcttgccg aagcctatca tcgttatggc tgtcccatct tcgtcgcgga
     1261 aaccagcatc gagggccgat cacgctccgc gtggttacgt tacgtatgtt cagaggtgta
     1321 cgaggccatc gagcagggcg tgcccgtgga aggcatctgc ctctacccgg tcatgagcca
     1381 ccccggctgg gacgatggcc ggtactgctc gaacggcttg ttcgaaatgg cgtggcgtag
     1441 cggcaggcgg gaggtttgca caccccttgc agaggaactg agatcgcagc agcagatcac
     1501 ttcggggttg cttggaagcg gggcgaaccg tgc
//
LOCUS       DASWQN010000266         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1478979, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000266 DASWQN010000000
VERSION     DASWQN010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478979"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VGC82_04395"
     CDS             <1..358
                     /locus_tag="VGC82_04395"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY0422543.1"
                     /translation="DPLNEYKSEAFALFNAMLAELKERVTGMLARVELAPERPMQPPP
                     MMQMVESHAEPQSALSSELALAEPAAPPRMVTATPNRTQAVDPNDQATWAATPRNALC
                     PCGSGKKYKHCHGRLG"
     gene            complement(391..1287)
                     /locus_tag="VGC82_04400"
     CDS             complement(391..1287)
                     /locus_tag="VGC82_04400"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0422544.1"
                     /translation="MTRLDAQLVQRFREDGFLSFDTGLGARDIAALRHILTTLHNKKV
                     GFEEGALFDALGIDDGSAPAFPQILHPRSFAPELLDNDFYSVAHGIAEQLLGKGVRFK
                     ADISLLKPARTGKATPWHQDEAFQDPAFDYAEVSFWLPLHPVDVTNSCMAYVPGSHKG
                     PVLPHGFPGGDERIHALECVGGFDPRDAVFCPLPLGGCVIHSGRTVHGAGPNISDQDR
                     MAYVLIFDRVPTPARLPRHFPWREQHRTARAKRELEWRRSGGLLVHLWRQRTRMRITS
                     VKTLAFDLRRAARAVRRIVLAG"
     gene            complement(1296..1547)
                     /locus_tag="VGC82_04405"
     CDS             complement(1296..1547)
                     /locus_tag="VGC82_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422545.1"
                     /translation="MVTDLPDPGSTGSGSGEAVTVSVSKIAARSHDVVSERAIFREIK
                     LFGSRATRFVIGFFADQGVFSLLISTFILDPDAGRKKKG"
     gene            1613..>1884
                     /locus_tag="VGC82_04410"
     CDS             1613..>1884
                     /locus_tag="VGC82_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422546.1"
                     /translation="MLRLSKTTTGTVLFGLDAALVVAVWPTVLWIARPDVLALLRLPA
                     DARGLHYPAFDLLLLFAMGLYRRDAILQAGRSLARVPLVVGMGATLA"
BASE COUNT          377 a          579 c          569 g          359 t
ORIGIN      
        1 cgacccgctg aacgagtata agtcagaagc tttcgccctg ttcaacgcca tgctggccga
       61 attgaaggaa cgggtcacgg ggatgctcgc gcgggtggag ttggctccgg agcggccgat
      121 gcagccgccg ccgatgatgc agatggttga aagccacgcc gaaccgcaat ccgcgctgtc
      181 gtcggaactg gctctggccg aaccggccgc accgcccagg atggtcacgg cgacaccgaa
      241 ccggacccaa gcggtcgatc ccaacgatca agccacgtgg gcggcaaccc cacgcaatgc
      301 gctctgtccc tgcggttcag ggaagaaata caagcattgt catggcaggc tgggttaagc
      361 tgggcgctgt cgccaccgga tcaacgcagt ctaaccggcc aggacgatcc gtcgcacggc
      421 tcttgctgcc cgccgcagat cgaatgccag cgtcttcacg ctggtaatgc gcatccgggt
      481 ccgctgccgc catagatgca ctagcaaccc tccgctgcgg cgccattcca gttcacgctt
      541 ggcacgggcg gtccggtgtt gctcacgcca ggggaagtgc cgaggcaggc gcgcgggcgt
      601 cggcacccgg tcgaaaatca gcacgtaggc catccggtcc tggtccgaga tgttgggacc
      661 ggcgccatgc acggtacgcc cgctatggat gacgcatccg cccaggggca gcgggcaaaa
      721 gaccgcatcc cgggggtcga acccgccgac gcactccaac gcgtggatcc gctcgtcacc
      781 gccgggaaat ccgtgcggca gcaccggccc cttatgcgag cctgggacat acgccatgca
      841 actgttggtc acgtccactg ggtgcagcgg cagccaaaag ctaacctcgg cgtagtcgaa
      901 cgctggatcc tgaaacgcct catcctggtg ccacggtgtt gccttgccgg tcctggctgg
      961 cttcagcaac gaaatgtccg ccttgaagcg tacccccttc cccagaagct gctcggcgat
     1021 gccgtgtgcg accgaataga agtcgttgtc gagcagttcc ggagcgaacg atcttggatg
     1081 caggatctgt gggaaggccg gtgctgatcc gtcatcaatg cccaatgcgt cgaataaagc
     1141 gccttcctcg aaaccgacct ttttgttgtg caacgtggtc aggatgtgcc gcaaagcggc
     1201 gatatcgcgt gcgccaaggc cggtatcgaa cgacaggaag ccgtcctcac gaaaacgttg
     1261 gaccaactgc gcgtcaagac gggtcatcac gagagtcagc cttttttctt gcggccggcg
     1321 tcaggatcga gaataaaagt gctaatcaac aatgaaaata ccccttgatc ggcaaaaaat
     1381 ccaatcacga accttgttgc tctgcttccg aatagtttaa tttcgcgaaa aatcgcgcgt
     1441 tcggagacaa catcatgtga tcgcgcggct atcttggaaa ctgaaactgt gacagcttcg
     1501 ccagagccgg atccagttga acccggatcg ggtaaatctg ttaccatctc tcacattccg
     1561 gctatagaac actcctggac agccaagctt gccgatttgg accaggacca ggatgttgag
     1621 actctcgaag acgaccaccg gcacggtact gttcggactg gatgctgcgt tggtcgtggc
     1681 cgtctggccc acggttctat ggatcgcacg gcccgatgtt ctcgccttgc tgcggttgcc
     1741 ggcggatgcc cgtggcttgc actatccggc cttcgatctc ctgctgctgt tcgcgatggg
     1801 gttgtaccgg cgcgatgcca ttctgcaggc tggccggtcg ttggcccgcg tgccgctggt
     1861 cgtcggcatg ggggcaacac tggc
//
LOCUS       DASWQN010000267         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1347266, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000267 DASWQN010000000
VERSION     DASWQN010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347266"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VGC82_04415"
     CDS             <1..533
                     /locus_tag="VGC82_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEY0422547.1"
                     /translation="WLGQRCVLGLAAASKTTQNLIRTRECVLNLPSDAMAAQVNRIAR
                     TTGSDPVPDGKARRGYRYLGEKFEAAGLTPVSSLTVKAPRAMECPVQLEAELEEIHPI
                     ADKDHAWRGRSLALHVVVRKVHAATDILAEGEPDRIDPDKWRPLIMSFQQFYGLAPGK
                     VHGSELAKIPEAAYRA"
     gene            581..1849
                     /gene="hisD"
                     /locus_tag="VGC82_04420"
     CDS             581..1849
                     /gene="hisD"
                     /locus_tag="VGC82_04420"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY0422548.1"
                     /translation="MIRVLKQGATEADRKVRQTVESILEDISARGDAAVRALSAKFDK
                     WEPAAFRLRPDEIRSLMDSLPGQVIDDIRFAQAQIRRFAQAQKDSLRDIEVETLPGIR
                     LGHKNIPVNSVGCYVPGGRYPMVASAHMSVLTAKVAGVKRVAACTPPLNGIPPAATVA
                     AMALAGADEIYLLGGIQAVAAMGLGTETIAPVDMLVGPGNAFVAEAKRQLFGRVGIDL
                     LAGPTETLIIADDTADAEMCATDLLGQAEHGPTSPAVLLTTSRKIAEGIQAEIDRQLA
                     VLPTADIASVAWRDHGQVILCDTDEELLSEADRIASEHVQVLTAEPRWFLDRMTNYGA
                     LFLGKETNVAYGDKVIGTNHTLPTKKAARYTGGLWVGKFLKTVTYQQVSPEATAMIGE
                     YCSRLCAIENFAGHKEQADLRVRRYGGKNA"
BASE COUNT          419 a          658 c          762 g          366 t
ORIGIN      
        1 ggtggctcgg ccagcgctgc gttctcggcc tggccgcggc gtcgaaaacg acacagaacc
       61 tgatccgcac gcgggaatgc gtgctgaacc tgccctcgga cgccatggcg gctcaggtaa
      121 atcgcatcgc ccggaccacc ggcagcgatc cggtgccgga cggcaaggcg cgacgtggtt
      181 accgctacct gggtgagaag tttgaggccg ccggcctcac tccagtgtcg tcgctgaccg
      241 tcaaggcgcc ccgagcgatg gaatgtcctg tgcagttaga ggccgaactg gaggagatcc
      301 acccgatcgc cgacaaggat cacgcgtggc gcggccgcag cttggcgctg catgtggtcg
      361 tccggaaggt tcacgccgcg accgatatcc tcgccgaggg ggagcccgac cgcatcgatc
      421 cggacaaatg gcgcccgctg atcatgagct ttcaacaatt ctacggcctg gcgccgggga
      481 aagtgcatgg gtccgagctt gcgaaaatcc ccgaggccgc ctatcgcgcc tgagggatgg
      541 tgcccgaagg atggtgcctg aaggacgatg aggcgagcga atgatccggg ttttgaagca
      601 aggcgcgacc gaggcggacc gcaaggttcg ccagacggtt gaatctatcc tggaggatat
      661 ctccgcgcgc ggggacgcgg cggtgcgcgc gttgtcggcg aagttcgaca agtgggaacc
      721 ggccgcgttc cgccttcgtc cggatgagat tcggtcgctg atggacagcc tgcccggcca
      781 ggtgatcgac gacatcaggt tcgcccaggc gcaaatccgc cgctttgctc aggcacagaa
      841 agactcgctg cgggacatcg aggtcgaaac cctgcccggc attcgcctgg gtcacaagaa
      901 cattccggtc aacagcgtcg gttgctacgt tccgggggga cggtatccga tggtcgcctc
      961 ggcacatatg tcggtgctga ccgcgaaagt cgccggggtg aagcgggttg ccgcctgcac
     1021 gccgccgctg aacgggattc cacccgccgc aacggtggcc gcgatggcgt tggcgggggc
     1081 ggacgagatc tacctgctgg gcggcattca ggcggtcgcg gcgatgggat tgggcaccga
     1141 aacgatcgcg ccggtggaca tgctggtcgg ccccggcaat gcattcgtcg ccgaggcgaa
     1201 gcggcagttg ttcgggcgcg tcggcatcga tctgctggcc ggtccgactg agacgctgat
     1261 catcgccgac gacaccgccg acgcggaaat gtgcgccacg gatctgctcg ggcaggccga
     1321 gcatggaccg acctcgccgg cggttctgct gacgacgtca aggaagatcg ccgagggcat
     1381 tcaggcggag atcgatcgcc aactggccgt gctgccgacg gccgatatcg catccgtcgc
     1441 gtggcgtgac catggccagg ttatcctgtg cgacacggac gaggaactct tgtccgaagc
     1501 agaccggatc gccagcgagc acgtgcaggt gctgacagcc gaaccgcgct ggttcctgga
     1561 ccggatgacc aactacgggg cactgttcct gggcaaggaa accaacgtcg cgtatggcga
     1621 caaggtgatc gggacgaacc acacattgcc gacgaaaaag gccgcgcgat acaccggcgg
     1681 gctatgggtg ggaaagttcc tgaaaaccgt gacatatcag caggtgagcc ccgaggcgac
     1741 ggcgatgatt ggcgaatatt gttcgcgcct gtgcgcaatc gagaacttcg ccgggcacaa
     1801 ggagcaagcc gatctgcggg tgcgacggta tggcgggaag aacgcgtagg ccgcaccggc
     1861 aagtccaggc cggcacctca tcgacatgac gccggcggga tgtcgcgcac cgcgagccgg
     1921 ctgggcgtcc gaaacgagag tctccgttgc agccgcgatg gaaccctgtt tccagtcacc
     1981 gtgaagccgt tctagctttt gggaacgggc actatttcgg tgttcgcgat ggcaccttgc
     2041 tgggagtgat ccaagcacag cggtacgaga tgcggcgggc gcgtgtgttt cgcccttcct
     2101 tatttttaac gcggattcag aatgagtctc acgaaacgct gcggagcagg atcgtacccg
     2161 ggacccaccg gtacgcgcgg tcgatctggc ggtcgaagtt agcgg
//
LOCUS       DASWQN010000268         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2535008, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000268 DASWQN010000000
VERSION     DASWQN010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2535008"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VGC82_04425"
     CDS             <1..226
                     /locus_tag="VGC82_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY0422549.1"
                     /translation="PRSLAMATIGLADVYGLPVSTTHVLSSGVAGAMAANGSGLQWST
                     IKNMALAWVLTLPAAMAIAGSLYVILRQMF"
     gene            complement(244..768)
                     /locus_tag="VGC82_04430"
     CDS             complement(244..768)
                     /locus_tag="VGC82_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422550.1"
                     /translation="MMGQGRGFLLGLAAASAVLCALVGLEISSWQQDAPAYPVSARAV
                     TADTDRRSDAVETPNQHEEWLNEILSRPLFSAGRRPAEAIASVSRGLPRLTGIVVAGT
                     QRVAIFAGLSNDRPIVAQPGTHVGAYEVQTVTDTGVTVAGPEGITVIKPIFDANRPPA
                     PAVARQQAARTGPK"
     gene            complement(765..1343)
                     /gene="gspM"
                     /locus_tag="VGC82_04435"
     CDS             complement(765..1343)
                     /gene="gspM"
                     /locus_tag="VGC82_04435"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEY0422551.1"
                     /translation="MNADSWLTGQRGQALAVGIGLLALALVWFGVVDPVWSWYADRNQ
                     LLEQRTALQHRMRAVAATLPALRAAKPERGDAPAAIMLPGATDAVAAADLQERIQKMA
                     GSAGASLTAVETLPAVPMGSWHKVPLRISVNAPWPVLMDLLRSIEGSRTRILIDDVHF
                     HNVTVVSRPVLLPIQASMVLYGFRPAEAGAGS"
     gene            complement(1340..2332)
                     /locus_tag="VGC82_04440"
     CDS             complement(1340..2332)
                     /locus_tag="VGC82_04440"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEY0422552.1"
                     /translation="MLAGFCSWWLARITELLPSAWTNLSSRPRDGIVIDADRGREINA
                     SLRRHGVLSPVGLGAAARQAGRTPVLVRPPDNVVLVKRHTVPTAPRRQLEQLLRHELG
                     RITPFPAQDLFWRWDGQAKPDNRTRTDVTLTMVPRMALAETLAALENVGLKANFIEVG
                     PPDRPCLLPAGDVARRASGTGLVRGLAWGCAGLTIVVLALPLVLQAIALRNIDAAIAE
                     LQPTINQVEVLRRGIAAGDAGRTILAKEMERTGDVLQTLASITRILPDDTFLTDFSLR
                     ERQITLSGRSASAPRLITGLSADPAIRNPAFAAPVTRIDGSTSEVFSIRAEVAK"
BASE COUNT          449 a          823 c          863 g          426 t
ORIGIN      
        1 gccgagatcg ttggcgatgg cgacgatcgg tctcgcggac gtatatggct tgccggtttc
       61 cacgacccat gtgctatcga gcggtgtggc cggcgcgatg gcagccaacg gttctgggct
      121 gcaatggagc acgatcaaga acatggcgct ggcctgggtg ctgacattgc cggcagcgat
      181 ggcgatagcc ggatcgctgt acgtgatctt gcgccagatg ttctgatcca cgcaacggcg
      241 gccctatttt ggtcctgtcc gagcagcctg ctggcgggcg acggcgggag caggggggcg
      301 gttggcgtcg aaaatcggct tgatcacggt tatgccttcc ggtccggcaa cggtgacgcc
      361 ggtatcggtg acggtctgca cttcataggc gccgacatgg gttccgggct gcgcgacgat
      421 cggacgatcg ttcgagagcc cggcgaatat cgcgacccgc tgtgttccgg cgaccacgat
      481 cccggtcaag cgcggcaacc cgcgggacac gctggcgatg gcttcggccg ggcggcggcc
      541 cgcactgaac agtggccgcg acaggatctc gttcagccat tcctcatgct ggttcggcgt
      601 ttcaaccgca tccgaccggc gatcggtgtc tgcggtcacg gcccgcgcgg aaaccggata
      661 cgcaggcgcg tcctgctgcc acgaggaaat ctcgagcccg acaagggcgc aaaggacggc
      721 cgatgccgcc gccagcccga gcaaaaatcc ccggccctgt cccatcatga gcctgctccc
      781 gcctcggcgg gcctgaaccc gtacagcacc atcgacgcct ggatgggcag cagtacaggc
      841 cgggaaacca ccgtcacgtt atggaaatgc acgtcgtcga tcaggattcg ggtccgtgag
      901 ccctcgatcg agcgcagcag gtccatcagc accggccatg gcgcgttcac gctgatccgc
      961 aacgggactt tatgccagct gcccatcggc acggcaggaa gcgtttcgac tgccgtcagg
     1021 ctggcaccgg ccgaaccggc cattttctgg atgcgctcct gcaggtcggc ggctgccacc
     1081 gcatcggtgg cccccggcaa catgattgcc gccggcgcgt cgccccgctc cggcttggcg
     1141 gcccgtaaag cgggaagcgt cgcggcgacc gcgcgcatgc gatgctgcag cgccgttcgc
     1201 tgttccaaca gctggttgcg atcggcatac caggaccaaa ccggatcgac cacgccgaac
     1261 cataccagcg ccaaagccag aaggccgata ccaacggcca gcgcctggcc gcgctggccg
     1321 gtcagccacg aatccgcgtt catttcgcga cttccgcccg gatggagaac acttcggagg
     1381 tggagccgtc gatgcgggtc accggagcgg caaacgcggg gttgcggatc gccggatcgg
     1441 ccgaaagtcc ggtaatcagg cgcggtgccg acgcagaccg gccgctcagc gttatttgcc
     1501 tttcccgcaa ggaaaagtcg gtcaggaacg tgtcgtccgg caggatgcgg gtgatcgaag
     1561 cgagagtctg cagcacgtcg ccggttcgct ccatttcctt cgccaggatg gtccgtccgg
     1621 cgtcgccggc ggcgatcccg cggcggagta cctcaacctg gttgatggtg ggttgcagtt
     1681 cggcgatcgc ggcatcgatg ttgcgcaggg caatcgcttg caggaccagc ggcagcgcca
     1741 gaacaacgat cgtcaggccg gcacagcccc atgccaatcc tcggaccaac ccggtgccgc
     1801 tggcgcgacg ggcgacatcg ccggccggca acaggcatgg ccggtccggt ggaccgacct
     1861 cgatgaaatt cgccttcagg ccaacgttct cgagagcggc cagggtttcc gccagcgcca
     1921 tgcgcggtac catcgtcagg gtaacgtcgg tccgcgttct gttgtccggc ttggcctggc
     1981 cgtcccaccg ccagaacagg tcttgcgccg ggaacggcgt tatccgtccg agttcgtggc
     2041 gcaggagttg ctccagttgc cggcggggtg cggttggaac cgtatggcgt ttgaccagaa
     2101 cgacattgtc gggtggccgc accaggaccg gcgtccggcc ggcttgcctg gcggcggcgc
     2161 caagcccgac aggactcagc acaccgtgcc ggcgcaacga cgcgttgatt tcccggccgc
     2221 ggtccgcatc gatgacgata ccatcccgcg ggcgggaaga taggttcgtc caggcggaag
     2281 gcagcagctc ggttatgcga gcaagccacc aggaacaaaa accggccagc atgggtagga
     2341 tccctcgttt ggctcaatca accgaaccgg cctgaaatgc caatcgcgaa tgcgagaggt
     2401 gataccggag cccgaaggct gacccgtgtg cccgcggatt ggtgtgcccg cggattgggt
     2461 gcccggctga ctggtgtgcc cgcgggttgg tgtgcctgcg ggttggtgcg gcaactgcgc
     2521 accaaatcga caatatggcg ccatccaagg ccgatagcca g
//
LOCUS       DASWQN010000269         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_687395, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000269 DASWQN010000000
VERSION     DASWQN010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687395"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VGC82_04445"
     CDS             complement(<1..725)
                     /locus_tag="VGC82_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0422553.1"
                     /translation="MTDAAPSYMHGASDQPMIGDTIGVHFDRTVARFGNRPGLIVHQQ
                     GINWTWSELAQHVEAFAAGLISLGLQPGDRIGIWSPNNAEWIVTQFATAKAGLILVNI
                     NPAYRLTEVEYALNKVGCKALITASRFKTSDYIGMINTLAPELAISHPGELHAERLPS
                     LRSVIQIGGDDPGTLAFDKIAHHGREADRQRLKELASLLQFDDPINIQFTSGTTGLPK
                     GATLTHHNILNNGFFIGEVMRYT"
     gene            complement(909..1262)
                     /locus_tag="VGC82_04450"
     CDS             complement(909..1262)
                     /locus_tag="VGC82_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003625676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0422554.1"
                     /translation="MAKEVRMTVTAEKRNETRRSINWDRLRAMTDDEIDQAIIKDPDI
                     SPLTEAEGMALRLQAIRKRMNLSQSQFANRFHIPVATLRDWEQARRQPDAAVWAYIQV
                     IDHEPDAVIRALKAA"
     gene            complement(1241..1519)
                     /locus_tag="VGC82_04455"
     CDS             complement(1241..1519)
                     /locus_tag="VGC82_04455"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEY0422555.1"
                     /translation="MFMDISFDADKDAANLAKHKLPLSFGEDVLTNKHGEVEDQRRDY
                     GEKRMKAFSQINGLWFQCLYTMRGTVAHIINVHRVKETEMQRWLKKSG"
     gene            complement(1732..>1933)
                     /locus_tag="VGC82_04460"
     CDS             complement(1732..>1933)
                     /locus_tag="VGC82_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HEY0422556.1"
                     /translation="APLHYPHLSHTEIFESVEAFYRRFYFRAPKIASIVNEMVRSPQM
                     MKRRLREGVEFFQFLRERHHAA"
BASE COUNT          338 a          556 c          606 g          433 t
ORIGIN      
        1 tcggtgtagc gcatcacctc gccaatgaag aagccgttgt tcaggatgtt gtggtgcgtc
       61 aatgtcgcac ctttcggcag cccggtggtg ccggaggtga actggatgtt gatcggatcg
      121 tcgaactgca gcagcgacgc cagttccttc agtcgctggc ggtccgcctc ccggccatgg
      181 tgggcgatct tgtcgaacgc caaggtaccg ggatcgtcgc cgccgatctg gatgacagag
      241 cgcagcgacg gcagccgttc agcgtgcaac tcgcccggat gggagatcgc gagttccggc
      301 gccagcgtgt tgatcatgcc gatgtagtcg ctggtcttga agcgtgacgc ggtgatcagc
      361 gctttgcagc cgactttgtt cagggcgtac tcgacttccg tcaggcggta ggcggggttg
      421 atgttgacca ggatcagccc cgccttggcc gtggcgaact gggtgacgat ccactcggcg
      481 ttgttcggcg accagatgcc gatgcggtcg ccgggctgca gaccaaggga aatcaggccg
      541 gcggcaaagg cttcgacgtg ctgggcgagt tccgaccacg tccagttgat gccttgctga
      601 tggacgatca gccccggccg attgccgaag cgggcaacgg tgcggtcgaa atggaccccg
      661 atcgtgtcgc cgatcatcgg ctggtccgat gcgccatgca tgtagctggg cgcggcgtcg
      721 gtcatcggtc tgctctcccc aggatgttgg ttgtttgggg gctatcatgc ttgttggctg
      781 agttgcttac aacctgcggc cacgtaagac cgccctcgat gtggcgactg tccggctttg
      841 gcgcggattg acgccgtagc gtcgcaccga gcgagagtgg gcgcggttaa tgcagggcgg
      901 aggaagcatc aggccgcttt cagggcccga attactgcgt ccggctcatg atcgatcact
      961 tgaatatagg cccacacggc cgcatcgggc tgtcgccgag cctgctccca gtcccgcagc
     1021 gtggctacgg ggatatgaaa ccggttggca aattgactct gcgacaagtt catgcgcttg
     1081 cggatggctt gtaggcgaag ggccatcccc tcggcttcgg tcagcggcga gatatcaggg
     1141 tctttgatta tggcctgatc gatctcgtca tcggtcattg cccgcagccg atcccagtta
     1201 atcgatcgtc tggtctcgtt ccgtttttcg gcggttacag tcatccggac ttctttagcc
     1261 atcgctgcat ctccgtttcc ttaacccgat gcacgttgat gatgtgtgcg acggtgcctc
     1321 gcatcgtata taaacactgg aaccaaaggc cgttgatctg gctgaaggcc ttcatccgtt
     1381 tttcgccgta gtcgcggcgc tgatcctcaa cctcgccatg cttgttcgtc agtacgtctt
     1441 cgccaaatga cagcggaagc ttgtgcttcg ccaggttcgc ggcgtccttg tcagcgtcga
     1501 agctgatgtc catgaacatc tatatgggat tgccgtatag atgtcacgca atattggtga
     1561 tgatcagcgc tacgcgccca atcgccacac catgagcaac atcctgtgcg tggttcagtc
     1621 gtccttggac cgggattcca tcgataccat tgccggctca atcggcggcg cagtatagct
     1681 ggttgggcag actctttatc cgccgaccag caaagcgcga cgttccattc cctaagccgc
     1741 gtgatggcgt tcccggagga actggaagaa ctcgacccct tcccgtagcc ggcgcttcat
     1801 catctgcggg ctgcgcacca tctcgttgac gatcgaggcg atcttcgggg cgcggaagta
     1861 gaagcgccgg tagaacgcct ccacgctctc gaagatctcc gtgtgcgaaa ggtgcgggta
     1921 gtgcagcggc gcg
//
LOCUS       DASWQN010000270         5655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1083353, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000270 DASWQN010000000
VERSION     DASWQN010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083353"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..968
                     /gene="tpiA"
                     /locus_tag="VGC82_04465"
     CDS             231..968
                     /gene="tpiA"
                     /locus_tag="VGC82_04465"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY0422557.1"
                     /translation="MRQLIAGNWKMHCLASEAVALAQGVAAGAQGIAAELLVCPTALH
                     VAAVAQALAGTSIAVGGQDCHQARQGAHTGDISGPMLRDAGATWVILGHSERRQNHDE
                     TDELIREKTLAAVEAGLIPIVCVGETGDQRSGGQETEVVGWQLAGSLPKPFNGVVAYE
                     PVWAIGTGKTATDADVATMHGFIREELVRQFGDAGRTIRILYGGSVRPANAAALLAIP
                     EVGGALVGGASLNPQDFVAIARAAQAG"
     gene            1015..1383
                     /gene="secG"
                     /locus_tag="VGC82_04470"
     CDS             1015..1383
                     /gene="secG"
                     /locus_tag="VGC82_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY0422558.1"
                     /translation="MSTVLLIIHLFITLALIGVVLIQRSEGGGLGIGSSQGMGSFMSG
                     RGTANLLTRATAVLAVMFMGLSLTLALLNRGTSGPTRSILDTPAPVTSPSPASGSSPV
                     VPPAAPPPPAAPSGPSAPTN"
     gene            1473..3104
                     /locus_tag="VGC82_04475"
     CDS             1473..3104
                     /locus_tag="VGC82_04475"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY0422559.1"
                     /translation="MTRYVFVTGGVVSSLGKGIAAAALGALLQARGFKVKLRKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGVHATKLDNATTGRIYQDVIAKE
                     RRGDYLGATVQVIPHITDAIKEVVLRESAEFDFVLVEIGGTVGDIESLPFLEAIRQLG
                     NELGPGGAMFVHLTLVPYIPSAGELKTKPTQHSVKELLNVGIQPQMLLCRCDRPIPQN
                     ERRKIALFCNVRPSAVIAALDVATIYQVPIAYHAEGMDREVLRHFRLNADIEPDLSRW
                     RRIVDTVQLPEGEVHIAIVGKYTNLLDSYKSLSEALTHGGIANRVKVRLEWVDSEIFE
                     TPGAVHRLEGVHGILVPGGFGERGTTGKIAAVRFAREHKVPFFGICFGMQMAVIEAAR
                     NLAGMREASSTEFGPCDTPIVGLLTEWARGNQIERRSESDPMGGTMRLGAYPAVLGKD
                     SLVRSIYGNRPVIEERHRHRYEVNVNFQERLEATGLRFSGMSPDGVLPEIIELPGHPW
                     FIGVQYHPELKSKPFEPHPLFASFIGAAVRQARLV"
     gene            3179..3361
                     /locus_tag="VGC82_04480"
     CDS             3179..3361
                     /locus_tag="VGC82_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422560.1"
                     /translation="MSPPDDIPFEPSGSQSESSGTSKPGGSRREIGAESEVRGGPEAP
                     DDELDETADGPEKPKP"
     gene            3800..4342
                     /locus_tag="VGC82_04485"
     CDS             3800..4342
                     /locus_tag="VGC82_04485"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0422561.1"
                     /translation="MTNPYLTLSSRIAYENRWTRVREDRIRRPDGSDGLYGVVERSDF
                     VVIVPWHDGRLTLVEQYRYPVRARMWEFPMGMWEQAPGTDPGMLAAGELREETGLIAG
                     RMTLAGEIFQGPGYCNQRGHIFLATDLTQGETDRELSEQDMIARTFALAEFEAMVREG
                     LVREAMTISAFGLLRVKGLI"
     gene            complement(4422..4823)
                     /locus_tag="VGC82_04490"
     CDS             complement(4422..4823)
                     /locus_tag="VGC82_04490"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY0422562.1"
                     /translation="MPHADLDPADGLRGDHYGNHTTRTRQVTLMQTEHLGAIAAYLGR
                     DFIEPAMLRRNILVSGINLRALIKQEFRLGSAVLLATGECHPCSRMEKLLGPGGYNAV
                     RGHGGITARILGRGRVQLGDEIVRVDSMALP"
     gene            complement(5047..>5655)
                     /locus_tag="VGC82_04495"
     CDS             complement(5047..>5655)
                     /locus_tag="VGC82_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422563.1"
                     /translation="GIVAGVAFAMIATARLADIGAVWTEHNRDLAAFQQVIPGIRPAE
                     NVLAVAVLQTDIPRFNAGQPRSRFLRALNLTSIMHLPALVLIEHDAFWPLLFTAVAKQ
                     PLRVRPAYAGLAVDEGLVPSYRALEVAAAGGALPRDAPYLAHWQRDFDYVLLLHAGKL
                     PLQDQPLLPGRLELLKQADVAALYRVRRSDGPSQATPAIARP"
BASE COUNT         1041 a         1780 c         1839 g          995 t
ORIGIN      
        1 aactggacgc gcccccgttg caacaacgag ccggcgccgc gggcagccat cgggaggtag
       61 gatcatattc ccgcgatgat gcttgaatgg ccagatcctc gcccaacgcc gcccagaggg
      121 acgcaacatc gtctgaccgt catctgtcat accgaatacg caataatatg ctcttgacgc
      181 ctcgcccaat gcgttagcag catttccgcc gagcggaggg acacggaacc atgcgacaac
      241 tgatcgccgg aaactggaaa atgcattgtc tcgcgtccga ggcggtggca ctggcgcagg
      301 gcgtcgcggc cggcgcgcaa ggcatcgcgg ctgaattgct ggtctgtccc actgcgctgc
      361 atgtcgctgc cgtcgcacag gcgttggccg gcacctccat cgcggtcggc ggccaggatt
      421 gccatcaggc caggcaaggc gcccacaccg gcgatatcag cggcccgatg ctgcgggacg
      481 ctggcgccac atgggtcatc ctcggccatt ccgaacggcg gcagaaccac gacgagaccg
      541 atgagctgat ccgcgagaag acgctggccg ccgtcgaggc cggcctgata ccgatcgtct
      601 gcgtcggaga aactggggat cagcgtagcg gcgggcagga aaccgaggtg gtcggctggc
      661 aactcgccgg cagcctgccg aagccattca acggcgtcgt cgcctatgag cccgtctggg
      721 cgatcggaac cggcaagacc gcgacggatg ccgatgtcgc caccatgcac ggctttatcc
      781 gcgaagaact ggttcgccag ttcggcgatg ccggccggac catccgcatt ctgtatggcg
      841 gttcggtcag gcccgccaac gcagccgcgt tgctggcgat cccagaggtc ggcggcgccc
      901 ttgttggagg ggccagcttg aatccccagg actttgttgc gattgcccga gcggcccagg
      961 ccgggtaaga ggcgcgcggc ggcacatcgc cgccaggttt gaaagtcctt cgcgttgagc
     1021 acagtcctgc tgatcatcca tttgttcatt accttggccc tgatcggcgt tgtgctgatc
     1081 caacgcagcg agggcggcgg cctgggcatt ggcagttccc agggcatggg atccttcatg
     1141 tccggccgcg gcaccgccaa cctgctgacc cgagccacgg cggtcctggc ggtcatgttc
     1201 atgggattgt cgctgacgct ggcgctgttg aaccgcggga cctccgggcc gacgcggtcg
     1261 attctcgata ccccggctcc cgtcacctcg ccgtcgccgg catccggcag ctcaccggtc
     1321 gtgccgcctg ccgctccgcc gccaccggcc gcaccgtctg ggccatccgc gcctacgaac
     1381 tgatcgaccg attccaattg gttattgtca aattttaatt tccggtcagc gggcttcccc
     1441 tgccccgagt cggtcggcta tgatagtcgc ccatgacgcg atatgtgttt gttactggcg
     1501 gcgtggtctc atccctgggc aaaggaatcg cagccgcggc gctcggcgcc ttgctgcaag
     1561 cccggggttt caaggtcaag ctgcgaaagc tcgatcccta tctcaacgtc gatccgggca
     1621 ccatgagtcc ctaccagcat ggcgaggtct tcgtcaccga tgacggcgcc gaaaccgatc
     1681 tggatctggg acactacgag cgctttaccg gcgtgcatgc cacgaagctc gacaacgcca
     1741 ccaccgggcg catctatcag gacgtgatcg ccaaggagcg ccgcggcgac tacctgggcg
     1801 caaccgtgca ggtgatcccg cacattaccg acgccatcaa ggaagtggta ctgcgggaat
     1861 ccgcggaatt cgacttcgtc ctggtcgaga tcggcggcac cgtgggcgac atcgaaagcc
     1921 tcccgttcct ggaggccatc cgccaactgg gcaacgaact cgggccgggc ggcgcgatgt
     1981 tcgtgcacct gaccttggtg ccgtacatcc cttcggccgg agagttgaag accaagccga
     2041 cccagcactc cgtgaaggag ctgttgaacg tcggtatcca gccgcaaatg ctgctgtgcc
     2101 gctgcgaccg gccgatccca caaaacgagc gccgcaagat cgcgctgttc tgcaacgtgc
     2161 gtccctcggc ggtgatcgcc gccctggatg tcgccaccat ctatcaggtt cccatcgcct
     2221 accatgcgga aggcatggac cgcgaagtgc tgcgccattt caggctgaac gccgacatcg
     2281 aacccgatct gtcgcgctgg aggcggatcg tcgataccgt gcaactgccg gagggcgaag
     2341 tccacatcgc aattgtcggc aaatacacca acctgctgga cagctataag tcgttgtccg
     2401 aagcactgac ccatggcggg atcgccaatc gggtgaaggt ccgcctggaa tgggtggaca
     2461 gcgagatctt cgaaacgcct ggcgccgtgc atcgcctgga gggcgttcat ggcatcctgg
     2521 tccccggcgg cttcggcgag cgcggcacca ccggcaagat cgccgcggtc cgtttcgcac
     2581 gcgaacataa ggtcccgttc ttcggcatct gcttcggtat gcagatggcg gttatcgaag
     2641 ccgcacgaaa tctcgccggc atgcgcgaag cgtcctccac cgagttcggc ccctgcgaca
     2701 cgcccatcgt cggcctgttg accgaatggg cccgcggcaa ccagatcgaa cgccggtcgg
     2761 aatccgatcc catgggcggc acgatgcgtt tgggcgccta ccccgcggtg ctgggcaagg
     2821 acagcctggt gcgttcgatt tatggcaaca ggccggtgat cgaggaacgt caccgccacc
     2881 gctacgaagt gaacgtcaat tttcaggaaa ggctggaggc cacgggtttg cgcttctccg
     2941 gaatgtctcc ggacggtgtg ctgccggaga ttatcgagtt gcccggccac ccatggttca
     3001 tcggcgtgca ataccatccg gaactgaaat ccaagccatt cgagccacat cccctgttcg
     3061 cgagcttcat cggcgctgcc gtccggcagg ctcggttggt gtagcgctct gccgaacaac
     3121 cgccggattc ggcgaaaggc ggtgcggaac ctttgccttc gggatgtgtt ggaggaccat
     3181 gtctccaccc gacgatatcc cgttcgaacc gtcaggcagc cagagcgaaa gttccggtac
     3241 gtcgaagcca ggcggttcgc ggcgggaaat cggtgccgag agcgaggttc gaggcggacc
     3301 tgaggcacct gacgacgaac tcgatgagac cgccgacggc cccgagaagc caaagccctg
     3361 accggtcacg cgctgcgcga tcgaggacct tgttaccggt aggcgcccgg tgtgacgggg
     3421 atcgtgcccc gaaaccgccg gttgaacgtc gaaaggtcgc caaacccggc gtccagtgcg
     3481 atgtccagaa tgggcccact cgaacggcgc agttcgattg cggcatgatg aaggcgcgta
     3541 cgcaggatgt actggcgagg cgtcaggccg aaatgagcgt cccagtatga gcgtgtgagt
     3601 gttgagccct gtcagcgggc cgtaggcgcc gacccgccag ggctgccttc tgacgtgtac
     3661 caccattttc acgctgtact tcgcctggat atgctctatc tcctcacgaa atccacttcg
     3721 gcgtagacac gctccgggtg tccgtctggc gcggcctcag ggttgccctc cacctcttga
     3781 ccgtgggcag tgcaagccga tgaccaatcc gtatctcacg ctgtccagcc gtatcgccta
     3841 tgagaatcgc tggacccggg tgcgtgagga tcggatacgg cggccggatg gttcggacgg
     3901 gttatatggc gtcgtggagc gctcggattt cgtggtcatc gtgccatggc atgatgggag
     3961 gctgaccctg gtggagcagt accggtatcc ggtccgtgcc aggatgtggg agttcccgat
     4021 gggtatgtgg gagcaagcgc ccggcaccga tccgggaatg ctggccgctg gcgaactgcg
     4081 ggaagaaacc ggtctgatcg ccggccgcat gaccctcgcg ggggagattt tccagggtcc
     4141 gggatactgc aaccagcgcg gccacatctt cctggcgacg gatctgacac agggcgagac
     4201 ggatcgggaa ctgagcgagc aggacatgat cgcgcggacg ttcgcactgg cggagttcga
     4261 ggcgatggtc cgggagggtt tggtgcggga ggcgatgacg atctcggcct ttgggctgct
     4321 gcgcgtgaag ggattgattt agctcccgcg cccgatatgg cggactggag gattgcggca
     4381 atcgtggcga ccacaactgc cctgatgagg gcggaagggc ctcaaggcaa cgccatgctg
     4441 tccacgcgga cgatttcgtc acccaactgc actcggccgc ggccaagaat ccgggcggtg
     4501 ataccgccgt gaccacggac ggcattgtaa ccgccggggc caagaagttt ctccatccgc
     4561 gagcagggat ggcactcgcc ggttgccagc aaaaccgccg atcccaatcg gaactcctgt
     4621 tttatcagcg cccgcaggtt gatgccggag acgaggatgt tgcgccgaag cattgccggc
     4681 tcgataaaat cgcggccgag ataggcggcg atcgcaccga gatgctcggt ctgcatcaag
     4741 gtgacctgcc gggtccgggt ggtgtggttc ccgtagtgat cgccacggag accgtcggcc
     4801 ggatcaaggt cggcatgtgg catggccaga agcggttccc gccgaacggg cctcaagccg
     4861 atccaggcga cgagcccggg acgcatgggc gctgccagaa gcgcggcaag ggacgaagca
     4921 ggattgagca gggtttggtc ctccgtcatc tttctgtggc gggaaggggc gagacaggct
     4981 tggcagttta tctaccagaa acaggccctc ccggcccagg tcgtcagccg actggcttcg
     5041 cctgattcac ggcctcgcga tagccggcgt ggcttggctt ggcccgtcgg agcggcggac
     5101 ccggtacagc gccgcgacat cggcctgctt caggagttcc agccggcccg gaagcagcgg
     5161 ttggtcctgt agcggcagct tgccggcgtg gagcagcagc acgtagtcga aatcccgctg
     5221 ccaatgcgcc agataaggcg catcccgcgg caacgctccg ccagccgccg cgacttccaa
     5281 tgctcgatag gatggcacca agccctcgtc caccgcgagt ccggcatagg cgggacgcac
     5341 tcgcagcggc tgtttcgcga ccgcggtaaa cagcaggggc cagaatgcat cgtgttcgat
     5401 cagtaccagc gccggcagat gcatgatcga tgtgaggttc agcgcccgca agaaacggct
     5461 tcgcggctgc ccggcgttga atcgcgggat atcggtctgc agaaccgcca ccgccaggac
     5521 attctcggcg ggccggatgc cggggatcac ctgttggaac gccgcgagat cgcggttgtg
     5581 ctccgtccag accgccccga tatcggccaa ccgtgccgtc gcgatcatgg cgaaggcgac
     5641 accggcaaca atgcc
//
LOCUS       DASWQN010000271         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1083417, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000271 DASWQN010000000
VERSION     DASWQN010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083417"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            759..2654
                     /locus_tag="VGC82_04500"
     CDS             759..2654
                     /locus_tag="VGC82_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HEY0422564.1"
                     /translation="MTGRYQELHSRSLTDPEGFWGEAAEDITWIKRWDSVLDGSNPPF
                     YRWFKGGVLNTCYNAVDRHVEAGRGEQNALIYDSPVTGTVQAITYGELRDQVARFAGV
                     LRGLGVEKGDRVIVYMPMIPQAAVAMLACARLGAVHSVVFGGFAPHELATRINNAKPK
                     VIVSASCGIEGSRVIKYKPMLDAAIEQSVHKPDHCVVFQRPQETASLIEPRDLDWVTA
                     EPAECVPVEATDPLYILYTSGTTGQPKGIIRDNGGHAVALKWTMSNMYDIKPGEVFWA
                     ASDVGWVVGHSYIVYAPLLHGATTVLFEGKPVGTPDAGTFWRTIARHKVSALFTAPTA
                     FRAIKGQDPHAEFMKKCDLSSLRALFLAGERSDPDTLHWAEDNLKVPVIDHWWQTETG
                     WCIAGNPLGIELLPVKYGSATKALPGWDVRCVDVSNQEVKRGDIGAIVIKLPLPPGTL
                     TTLWNADDRFVKSYMTDYPGYYQTGDAGFIDDDGYVYIMARTDDIINVAGHRLSTGAM
                     EDVLSAHKDVAECAVIGVADQLKGQVPLGLLVLKNGVTRSPDDIVKDVVRMVRDEIGP
                     VAAFKLAIVVDRLPKTRSGKILRGTMQKIADSEQYKMPATIDDPAVLSEISDALQTIG
                     YAKPGMI"
BASE COUNT          552 a          901 c          844 g          504 t
ORIGIN      
        1 gagatcgtca cggagatcca ttctgaagaa caatacgtcg ccgataacct tcatattcgc
       61 catcccatcg aaaagcctct cgatagacag cgttacaacg ccttttatta aaaaatagag
      121 agcggccgaa gggtactgct ccccgcgcac ttcgcccagc caatgccggt tactctgcca
      181 gcccagcggt cagacgctga acagccgtta cggaaaagca caccgccggt tgctgcgtat
      241 ggagatccct agctgtggcg ctgtcgcccc gtcattgtga cagcgccatc tcgaacagcc
      301 ggtccggatc atcgatccag atggccgaaa tgcccatcgc ctctgcatag cggcgggcta
      361 tgccgaaagc ctgcgccact gggacgccta atgatgactt ggcaaattta aagctatcgg
      421 aagcgggtcg tgtggtcgac cgacacgccc cagtcccggg gcatcagttc gaggtcgcgg
      481 taggagatcg gaaactgcag gtaccagcgc agtgctcaca ggatcacttc cgtcgtgaat
      541 tgccggccct tgaagacacc cgtctccgtc atcctccacc catcggaatt cgctaccgtg
      601 ccggcggtct atcggatcca gaccctgagt caaacttgca tcagaaccgt ttgcaaagct
      661 tcatggcttt gaagcactat cctcgcacat tccggataat ttaggacgcg tgaagggacc
      721 tgccaccacc gctgaggcca agaacaagaa ggcgttccat gaccggtcga tatcaggagc
      781 ttcatagccg ctcgctcacc gaccccgaag gtttctgggg agaggccgcc gaggacatca
      841 cctggatcaa gcgctgggac agcgttcttg atggatccaa cccgccgttt taccggtggt
      901 tcaagggtgg cgtactgaac acgtgctaca acgccgttga ccgtcacgtc gaagccggcc
      961 gcggcgagca gaacgcgctg atctatgaca gtccggtcac cggcacggtc caggccatca
     1021 cctacggcga actgcgcgat caggtcgccc gtttcgccgg agtgctgcgc ggcctcggcg
     1081 tcgaaaaggg tgatcgggtc atcgtctaca tgccgatgat cccgcaggcg gcggtggcca
     1141 tgctggcctg cgcccggttg ggtgccgttc attccgtggt gttcggcggt ttcgcgccgc
     1201 acgaactggc cacccgcatc aacaacgcca agccgaaggt catcgtatcg gcctcgtgcg
     1261 gcatcgaggg cagtcgcgtc atcaagtaca agccgatgct ggatgctgcc atcgagcaat
     1321 cggttcacaa gcccgaccat tgcgtcgtgt tccagcgccc gcaggaaacc gcgagcctga
     1381 tcgaaccgcg cgacctcgat tgggtcacgg cggagccggc ggaatgcgtg ccggtcgaag
     1441 cgaccgatcc gctctacatc ctgtacacct ccggcaccac cggtcagccc aagggcatca
     1501 tccgtgacaa tggcggtcat gccgtggcgc tgaaatggac gatgtcgaac atgtacgaca
     1561 tcaagccggg cgaggtgttc tgggccgcgt cggatgtcgg ctgggtggtc ggccattcct
     1621 acatcgtcta tgcgccgctg ttgcacggcg ccaccaccgt cctgttcgaa ggcaagccgg
     1681 tcggcacgcc cgacgccggc acgttctggc gcaccatcgc ccggcacaag gtttcggccc
     1741 tgttcaccgc accgacggcg ttccgcgcca tcaagggcca ggatccgcac gccgaattca
     1801 tgaagaagtg cgatctgtcg tccctgcgcg cgctgttcct ggccggcgaa cggtccgacc
     1861 cggacaccct gcactgggcc gaagataacc tgaaagtgcc ggtgatcgac cactggtggc
     1921 agactgaaac cggctggtgt atcgccggta acccgctggg gatcgagctg ctcccggtca
     1981 agtacggttc cgcgaccaag gccctgccag gctgggacgt ccgctgtgtc gatgtttcca
     2041 accaggaggt caagcgcggc gatatcgggg ccatcgtgat caagctgcca ctgccgccgg
     2101 ggaccctgac gacgctgtgg aacgccgacg accggttcgt gaagtcctac atgaccgatt
     2161 atcccgggta ctaccagacc ggtgatgccg gcttcatcga tgacgacggc tatgtctaca
     2221 tcatggcccg caccgacgac atcatcaatg tcgccggcca ccgcttgtcc accggcgcca
     2281 tggaagatgt gctgtccgcc cacaaggacg tggcggagtg cgccgtgatc ggcgtcgccg
     2341 accagctcaa gggtcaggtt ccgctcggct tgctggtgct gaagaacggc gtcacacgca
     2401 gccctgacga catcgtcaag gacgtcgtca ggatggtccg cgacgagatc ggcccggtcg
     2461 cggcgttcaa gctggcgatc gtggtggacc gcctgcccaa gacccgctcc ggcaagatcc
     2521 tgcgcggcac catgcagaag atcgccgaca gcgagcagta caagatgccg gcgaccatcg
     2581 acgatcccgc cgtcctgagc gagatctccg acgcgctgca gaccatcggc tacgccaagc
     2641 cgggcatgat ctgatcctga accgcgtcca gattgagagt cgtggatttt gaacggcggc
     2701 ccggccgatg gtgtgttgca gtgtgtgaca cgacgcatcg ccgtcagcgc ccgatcagcc
     2761 ggcgccacca cggccgctat ggtggggtta tctgcccggc c
//
LOCUS       DASWQN010000272         4264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_292398, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000272 DASWQN010000000
VERSION     DASWQN010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292398"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VGC82_04505"
     CDS             complement(<1..645)
                     /locus_tag="VGC82_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEY0422565.1"
                     /translation="MIVGVPVLVGLIGTALLFDFLNGLHDSANSIATVVATRVLRPLT
                     AVLWAAFFNFIAFAVFGLHVAATMGSGVVDAATVDPPVVFGALAGAIVWNLVTWWQAI
                     PSSSSHALVGGLIGAGMTKAGPAAVVWGGIGKIGAAIVLSPLIGLLLAMTFVLVTTWM
                     AVRSMPFAVDRRFRVLQFVSSSMLSLAHGGNDAQKTMGIITVLLYSQGLLTGGFH"
     gene            complement(651..1313)
                     /locus_tag="VGC82_04510"
     CDS             complement(651..1313)
                     /locus_tag="VGC82_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEY0422566.1"
                     /translation="MAEFKRPNILFRAYRSLMPQDDRFIGWFCEHSRLMVPATVAFRA
                     LMSGDGRAEQHAVEIGRLEEAADEVTRQTIRAIHRTFVTPFDRSQILDLITALDDTID
                     LMKDTGRRMVRYGVGFTAEMRGMADCAVRAATEIRDGMPLLGSIDRNVERLSAMSVAV
                     RRIEGEADALLDQGLRVLFAGDTSPGHKLTVEKVYDLVEAVVDRCEDVVDVIDGIIVE
                     QV"
     gene            complement(1363..2229)
                     /locus_tag="VGC82_04515"
     CDS             complement(1363..2229)
                     /locus_tag="VGC82_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0422567.1"
                     /translation="MPNDDNRPQPPQPEQHQEPPGVTAEMTPKPDHGEHSYKGSGRLA
                     GKAAIITGSDSGIGRAVAIAFAREGADVLISYLQEDDDAKETQRWVEQCGRRAILMPG
                     DVSDESHCQSIVDRAVQEFGKLDILVNNAAMQRTHQSIDEISSEEWDQTFRTNIYSMF
                     YLSKAALRHMEPGAAIVNTTSINAKNPSPSLLAYATTKGAIANFTAGLAQLVAEKGVR
                     VNCVAPGPIWTPLIPSTMPEEHVSSFGKNTPLGRAGQPAELAPAYVLLASDEASYMTG
                     ALLPVTGGRPML"
     gene            complement(2273..2869)
                     /locus_tag="VGC82_04520"
     CDS             complement(2273..2869)
                     /locus_tag="VGC82_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEY0422568.1"
                     /translation="MNPREAILAAVRANLPDGHHERPVLPRFPLSQPEGPIASFERNL
                     KQMGGQLLEHDADTDPLTALQNRLRSAAIVCSAAPEITGNRDLATIEHPRDLADVDVA
                     VVRAAFGIAETGSVLFTEAELRVNALAYLAQHLIVLLDPTDIVASTQDAYRRRELHEA
                     RYAVFHTGPSATADIEGVLIHGAQGVRSLTVVPLPRQT"
     gene            complement(2856..4244)
                     /locus_tag="VGC82_04525"
     CDS             complement(2856..4244)
                     /locus_tag="VGC82_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008853304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HEY0422569.1"
                     /translation="MDQNEAAARFIAAPEHAKMHDERLWDLRKKRDAQMHGIPEWEDL
                     RELASQIKQHTLGHLDRYLEQFEENAKANGVHVHWAVDGAAHNQIVHGILRDHGAKRL
                     IKSKSMLTEECGFRHYMASTGIEVIETDLGERIQQLDNEAPSHVVVPAVHKLRADVAE
                     VFSRTIGTDPHNEDVNYLAGQQREATRPLILAADAGMTGANFAVAETGSFVVCTNEGN
                     ADLSANVPPLHIASIGIEKLIPRIEDLGIFIRLLSRSALGSPITQYTSHFRRPREGAE
                     MHIVLVDNGRSARLGMEEFWTSLKCIRCGACMNTCPVFRRSGGLSYGATYSGPIGLIL
                     DPTFDKKKYSSLPFSSTLNGSCTNVCPVKINIHEQIFAWRKALAEEHEIPLLKHQMMK
                     AAGTALSHPALYRAAIATADSALRHLPKFVVYNRFNTWTTGRDMPPVPAETFHAWYAR
                     HYHVEGRADEPA"
BASE COUNT          786 a         1343 c         1310 g          825 t
ORIGIN      
        1 gtggaagcca ccggtcagca gcccctggga atacagcagc accgtgatga tccccatggt
       61 cttttgcgca tcattgccgc catgcgccaa gctaagcatg gacgacgata cgaactgcaa
      121 gacgcggaat cgccggtcca ctgcgaacgg catggaccga acggccatcc acgtcgtgac
      181 cagcacaaag gtcatggcga gcagcaggcc aatcagcggc gacagcacga ttgcggcgcc
      241 gatcttgcca atgccgcccc aaaccaccgc ggccggccct gccttcgtca tgcccgcacc
      301 gatcaagcca ccgaccaggg cgtggctgct gctggagggg atggcctgcc accaggtgac
      361 caggttccac acgatcgcgc cggccagcgc gccgaacacc acaggcggat cgacagtggc
      421 ggcatcgacg acgccgctgc ccatcgtagc tgccacgtgc aagccaaaca ccgcgaaggc
      481 gatgaagttg aagaacgctg cccacagcac agcggtcagg ggtcgcagga cacgggtagc
      541 aaccacggtc gcgatcgagt tcgcgctgtc gtgcaacccg ttgagaaaat cgaacaacag
      601 ggccgttccg atcaggccga ccaacaccgg caccccgacg atcaccgcgg ctagacctgt
      661 tccacgataa tgccgtcgat gacatcgacg acatcttcac accggtcaac cacggcctcc
      721 acgaggtcgt aaaccttttc cacagtcaat ttatggcctg gcgaggtgtc gcctgcgaac
      781 agcacccgta gcccctgatc caacagtgcg tcagcctcgc cttcgatgcg ccggacggcc
      841 acggacatgg cgctgagccg ttcgacgttg cgatcgatcg atcccaacag cggcatgcca
      901 tcgcgtattt cggtggcggc ccgcaccgcg caatccgcca tgccgcgcat ctccgcagtg
      961 aagccgacac cataccggac cattcgacgg ccggtgtcct tcatcaggtc gatcgtgtcg
     1021 tcgagtgccg tgatcagatc caggatctgc gaacggtcaa acggcgtcac gaacgtccga
     1081 tggatggcac gtatggtttg ccgcgtgacc tcgtccgccg cttcctccag tcggccgatt
     1141 tcaacagcgt gctgctctgc ccgcccatcg ccggacatca acgcgcggaa ggcaacggtc
     1201 gcaggcacca tcagcctgga gtgctcgcag aaccagccga tgaaacgatc gtcctgcggc
     1261 atcagggagc ggtaagcgcg aaacagtatg ttcggccgtt tgaactccgc catttatctc
     1321 ctttcgtccc accgccaaac ggcccgccgc cgattcccgc gattacagca ttgggcggcc
     1381 acccgtgacc gggagcagcg cgcccgtcat gtagctggct tcatccgatg ccagcagcac
     1441 gtatgccggc gcgagttccg ctggttgccc cgcgcggccc agcggcgtgt tcttgccaaa
     1501 gcttgatacg tgctcttccg gcatcgtgga cgggatcagc ggcgtccaga tcgggccagg
     1561 tgcgacgcag ttcacccgca cgcctttctc ggccaccaac tgcgcgaggc cggcggtgaa
     1621 gttggcgatt gcgccttttg tcgtggcata ggcaagcagg ctgggggacg ggtttttcgc
     1681 gttgatcgag gtcgtgttca ctatggccgc accaggctcc atatgccgaa gcgccgcttt
     1741 cgaaagatag aacatcgagt agatattggt ccggaaagtc tggtcccatt cctcggaact
     1801 gatttcgtcg atgctttggt gggtgcgctg catcgcggcg ttgttgacca ggatgtcgag
     1861 cttgccgaac tcctgtaccg cccgatccac gatcgactgg cagtgggatt cgtccgacac
     1921 gtcgccgggc atcaggatgg cgcggcggcc gcattgttcg acccaacgct gggtttcctt
     1981 ggcgtcgtca tcctcctgaa gatacgaaat caggacatcc gctccttcgc gagcgaaagc
     2041 gatcgccaca gcacggccga ttccagagtc tgatccagta atgatcgcgg ctttgccggc
     2101 cagcctgccg cttcccttgt aggaatgttc gccatggtca ggcttcggcg tcatctccgc
     2161 cgttacaccc ggtggctcct ggtgctgttc cggctgcggg ggctgcgggc ggttgtcgtc
     2221 gttcggcatt attctttatc ctttcagtag tggtcggatc ggccgtagcc attcaggttt
     2281 ggcgcggcag cggcaccacg gtcagggaac gaaccccttg tgcgccgtgg atcagaaccc
     2341 cctcgatatc tgcggtcgcc gagggtccgg tgtgaaaaac cgcgtaacgt gcttcatgca
     2401 gttcgcgacg gcggtaagcg tcctgcgtcg atgccacgat gtccgttgga tcgagcagca
     2461 cgatcagatg ctgggcaaga taggcaaggg cgttcacccg aagctctgct tcagtgaaca
     2521 gcaccgaacc ggtctcggcg atcccgaacg ccgcacgcac gacagcaacg tccacgtcgg
     2581 caagatcccg cggatgctcg atcgtcgcca gatcgcggtt gccggtgatt tcgggtgccg
     2641 ccgaacagac gattgctgcg ctccgaagcc ggttttgaag cgctgtcaac ggatcggtgt
     2701 cggcgtcgtg ctccagcaac tgtccgccca tttgcttcag gtttcgctcg aagctggcaa
     2761 tgggtccttc cggctgcgaa agtgggaaac gaggcagaac agggcgttcg tgatggccat
     2821 caggaagatt ggcacgcact gccgcgagaa tggcctcacg cgggttcatc ggcccgtcct
     2881 tcaacgtgat agtggcgcgc gtaccaagcg tggaacgttt ccgccggcac cggtggcata
     2941 tcgcgtcccg tggtccaggt gttgaaccgg ttgtagacca cgaacttcgg cagatgccgc
     3001 agcgctgaat cggccgtcgc gatcgccgcg cggtatagcg cgggatgcga cagagccgtg
     3061 cccgccgcct tcatcatctg gtgtttgagc agcgggattt cgtgctcctc ggcaagggcc
     3121 ttgcgccagg cgaatatttg ctcgtggatg ttgatcttga ccggacagac attcgtgcag
     3181 gagccattca acgtggagga gaacggcagc gaagaatact tcttcttgtc gaaggtcggg
     3241 tccaggatca gcccgatcgg ccctgaatag gtcgccccat aggacaggcc gccactgcgg
     3301 cggaataccg ggcaggtgtt catgcaggcg ccgcagcgga tgcatttcag cgacgtccag
     3361 aactcctcca tcccaagcct ggcggaacgg ccgttgtcca ctaggacgat gtgcatctcg
     3421 gcgccttcgc gtggccggcg gaaatgcgag gtgtactgtg tgatcggcga gcccagcgcg
     3481 ctgcgcgaca gcaggcggat gaaaatgccc aggtcctcga tcctgggaat tagcttctcg
     3541 atgccgatcg aggcgatgtg cagcggcggc acgttggccg acagatcggc attgccctcg
     3601 ttcgtgcaga cgacgaacga tcccgtctcg gcgacggcga agttcgcgcc ggtcattccg
     3661 gcgtcggcgg ccaggatcag cggtcgcgtc gcctcccgtt gctgaccggc aaggtaattc
     3721 acgtcctcgt tgtgcggatc ggttccgatc gttcggctga acacctccgc gacatcggcg
     3781 cgcagcttgt gcacggccgg aacgacgaca tgactgggag cttcgttgtc gagttgctgg
     3841 atgcgctcgc cgaggtcggt ttcgatgact tcgatgccgg tgctcgccat gtagtgacgg
     3901 aagccgcact cctcagtcag catggatttc gacttgatca gccgcttcgc gccgtggtcc
     3961 cgcagaatgc catgcacgat ctggttgtgc gccgcgccat ccactgccca gtgcacatgg
     4021 acaccgttgg ctttggcatt ttcctcgaac tgttccaggt agcgatcgag atgcccgagg
     4081 gtgtgttgct taatctgcga ggcaagttcg cgaaggtctt cccattccgg aataccgtgc
     4141 atctgcgcgt cgcgcttctt gcgcaaatcc cacagccgct cgtcgtgcat cttggcgtgt
     4201 tcaggggcgg caataaagcg cgcggccgcc tcgttctggt caatcggccg ccgtccatgc
     4261 actt
//
LOCUS       DASWQN010000273         3639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_28935, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000273 DASWQN010000000
VERSION     DASWQN010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28935"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VGC82_04530"
     CDS             complement(<1..751)
                     /locus_tag="VGC82_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0422570.1"
                     /translation="MMKTVRTPILEIGFLELGPPDGPPVILLHGWPSDVHDYDGVAPP
                     LGEAGFRVLVPWLRGYGPTWFLDPATPRSGQQAALGADVRDFMDALAIPRATLVGYDW
                     GGRAACVAAALWPERVSALVSITGYGIQDIAASARPGSAEQEHRYWYQWYFHTERGRA
                     GLTANRVEMTRLLWRLWSPNWVFDDATLRATTASFDNPDFVDVTIQSYRHRYGNAPGD
                     PAYDDLEVRLAAQPPIGAPTIVLHGEDDGVGP"
     gene            complement(751..1125)
                     /locus_tag="VGC82_04535"
     CDS             complement(751..1125)
                     /locus_tag="VGC82_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0422571.1"
                     /translation="MLLDGKLALTTGGGSGIGEGIALRNPAAYTTSNGGITPLTKALA
                     IELSPQGVRVDAIGPGMIATPLNAAARQNPDYMANFTQRIPLGRLGEPADIAGPAVFL
                     ASDLARYVTGVTLPADGGFSAY"
     gene            complement(1127..1927)
                     /locus_tag="VGC82_04540"
     CDS             complement(1127..1927)
                     /locus_tag="VGC82_04540"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0422572.1"
                     /translation="MDFNGKVALITGAGNGIGRASALGFASRGAKVVVVDRDEAAGEA
                     TAGILRQQGAEAFFVVADVTRSADVQNYVKLALDKYGSIDCFHNNAGIEGTVAPTHQY
                     DEDMFDRVMAVNVKGVFLGLRHVLPVMLKQGSGAVVNTASVAGLVASPGMPAYVASKH
                     AVIGLTKTAAGEVARSGVRVNCVCPGPIDTRMIHSLESMLSPDDPASVGSRYQQNIPI
                     GRYGTAEEVANLVIFLSSSLASNITGAQYVVDGGRTATGGAVTNNLVR"
     gene            complement(1998..2825)
                     /locus_tag="VGC82_04545"
     CDS             complement(1998..2825)
                     /locus_tag="VGC82_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase family protein"
                     /protein_id="HEY0422573.1"
                     /translation="MAFVIGGIGSSHAPSIATAWDKGQQKDPLWASLFDGYVPVRKWL
                     DEVKPDLFLIVYNDHANRFFFDAYPTFALGVGAVHTQADEGWGKRNLPDLPGHPEFGW
                     HLARSLVDDEFDPTICQEMSVDHGVLSFLPMLTDSDWKIPVVPLAVNVIQHPIPLPRR
                     LFKLGQALRRAILSYPKDLRVVVFGTGGLTHQLSGAKFGTKNAAWDNEFMDLLETDPQ
                     ALARLSIQDYMDRGGTEGVEMIMWLAMRGALGSNVQRVHRNYTAPLLTGYGLLALTA"
     gene            complement(2827..3156)
                     /locus_tag="VGC82_04550"
     CDS             complement(2827..3156)
                     /locus_tag="VGC82_04550"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422574.1"
                     /translation="MSTAIADKYVFTGERSANSYRLNKMALTLTSPENRQKFQADEVG
                     YMRGLGLSDHEIDLVKRRDWKGMMDYGASIYLVIKIGGSVGQILPEIGAHTAGMTIAE
                     FHARRSH"
BASE COUNT          688 a         1200 c         1104 g          647 t
ORIGIN      
        1 ccggcccaac gccgtcatct tcgccgtgca ggacgatggt cggcgcgccg atcggcggtt
       61 gtgcggccag ccgtacttca agatcgtcgt atgccggatc gccaggcgcg ttgccgtagc
      121 gatgccgata ggattgtatc gtcacatcga cgaaatccgg gttgtcgaag ctggcagtgg
      181 tggcccgcaa tgtcgcatcg tcgaaaaccc agttcggcga ccacagccgc cacaacaggc
      241 gagtcatttc cacccgattg gcggtcagtc cggcgcgccc gcgttcggtg tggaaatacc
      301 attgatacca gtaacgatgt tcttgttcgg cggagccggg acgggcggac gcggcgatat
      361 cctgaatccc gtatccggta atcgaaacca gggcggagac ccgctccggc caaagtgccg
      421 cggccacgca ggcagcccgt ccgccccagt cgtaacccac cagggtcgcg cgtgggatcg
      481 ccaaggcgtc cataaagtcc cgcacatccg cccccagcgc cgcctgttgc ccgcttcgag
      541 gcgtcgcggg atccaggaac caggtcgggc cgtagcccct cagccacggc accagcaccc
      601 gaaaccccgc ttcgcccagc ggtggcgcga cgccgtcgta gtcgtgcaca tccgagggcc
      661 agccgtgcag caggatgacc ggtggcccat ccggcggtcc aagttctaaa aatccgatct
      721 ccaggatcgg cgtgcgaacg gttttcatca tcaatatgcc gaaaatccgc catccgccgg
      781 cagagtcacg ccggtgacat agcgagccaa atccgaggcc aggaataccg ccggcccggc
      841 gatatccgcc ggctcaccga ggcgcccgag cggaatacgc tgggtaaagt tcgccatgta
      901 gtcgggattt tgccgcgccg ccgcgttcag cggcgtggcg atcattccgg gaccaatagc
      961 gtccacccgc acgccttgtg gcgacagctc gatcgccagc gccttggtca gcggcgttat
     1021 cccgccgttc gacgtcgtgt aggcagccgg gttccgcagc gcgataccct cgccgatccc
     1081 cgatccgccg ccggtagtca gcgccagttt gccatcgagc aacatctcag cgcaccagat
     1141 tgttggtgac cgcgccgccc gtcgcggtgc gcccgccatc gacgacgtat tgtgcgccgg
     1201 tgatgttcga cgccaggctg gaggacagga agatcaccag gttggcgacc tcctcggccg
     1261 tgccataacg tccgatcggg atgttctgtt gataccggct gccgacgctg gccgggtcgt
     1321 ccgggctgag catcgattcc agggaatgga tcatccgcgt gtcgatcggt ccggggcaca
     1381 cgcagttcac ccgcacgccg gaccgggcaa cctcgccggc ggctgtcttg gtcaatccga
     1441 tgaccgcatg cttggacgcg acataggcgg gcatccccgg cgacgcgacc aggccggcga
     1501 ccgaggctgt gttcaccacg gcgccgctgc cttgcttgag catcaccggc agaacgtgtc
     1561 tcagaccgag gaacacgccc ttcacgttca ccgccatcac gcggtcgaac atgtcctcgt
     1621 cgtactgatg ggttggtgcg accgtgcctt cgatgccggc gttgttgtgg aagcagtcga
     1681 tcgagccata cttgtccagg gccagcttca cgtagttctg aacatccgcc gaccgggtaa
     1741 cgtcggcgac aacaaagaat gcttccgcgc cctgttggcg caggatgccg gccgttgcct
     1801 caccggcggc ctcatcgcgg tccaccacca ccactttggc gccgcggctg gcaaaaccca
     1861 atgccgatgc tcggccgata ccgttgccgg cgcccgtgat caaggcgact ttgccgttga
     1921 agtccatcgt ttcctcgctt gttgtgggcg ttgcgaggac gctagaccaa tcccggccgg
     1981 acgggaatta ggcggcgcta cgccgtcaac gccagcaggc cataccccgt cagcagcggc
     2041 gcggtgtagt tgcggtgcac ccgttggaca ttcgagccaa gcgccccgcg catcgccagc
     2101 cacatgatca tctcgacgcc ctcggtcccg ccccggtcca tgtaatcctg gatcgaaagc
     2161 cgggccagcg cctggggatc cgtttccagc agatccatga attcattgtc ccacgccgcg
     2221 ttcttggtgc cgaacttcgc gccgctgagc tgatgcgtca ggccgccggt gccgaacacc
     2281 acgacgcgca gatccttcgg atacgacagg atcgcccgcc gcaacgcctg gccaagcttg
     2341 aacagccgcc gaggcagggg aatgggatgc tggatgacgt tcaccgccag cggcacgacc
     2401 gggattttcc agtccgaatc cgtcaacatc ggcaggaacg acaacacgcc gtgatccacc
     2461 gacatttcct ggcatatcgt cgggtcgaac tcatcgtcca ccagcgaacg ggccaggtgc
     2521 cagccgaact ccgggtgccc gggcagatca ggcaaattcc gcttgcccca tccctcatcg
     2581 gcctgggtat ggaccgcgcc gacgcccagc gcgaaggtcg gataggcgtc gaagaagaaa
     2641 cggttggcat gatcgttata aacgatcaag aacagatccg gcttcacctc atcgagccat
     2701 ttacgtaccg gaacgtagcc gtcgaacaag gaggcccaaa gcgggtcttt ctgttgcccc
     2761 ttatcccagg ccgtggcgat cgaaggggcg tgcgagctgc cgatgccgcc gatgacgaat
     2821 gccatgtcag tgactccgcc gggcgtggaa ctcggcgatc gtcattccgg cggtatgagc
     2881 accgatctcg ggcaggattt gcccaaccga cccgccgatc ttgatcacga ggtaaatgct
     2941 ggcgccgtaa tccatcatgc ctttccagtc acgccgcttc accagatcga tttcgtgatc
     3001 cgagaggccg agaccgcgca tgtaaccaac ttcatcagcc tggaattttt gccgattctc
     3061 cggcgaggtc agcgtcagcg ccatcttgtt cagacggtag ctattggccg atctttcacc
     3121 cgtgaaaaca tatttgtcgg ctatggcggt gctcattcgc tgccctccct cgcctgacga
     3181 taacacggga actacgacgc cccaaggtgt ccacgccaag gcaatcgggt aagcgaattg
     3241 aagcccgcca acaggtcaag agttggtcca tcctgatccg tcagccctga tccgtcagcg
     3301 tccgtccgca actaagcgcg tcgggtcgag cgggccagga gatccgtctt cgtgggcaaa
     3361 gtccccaggg gctggaacac agcgctccag gggtgcccga gtctgctcca gcggtgcccg
     3421 agtctggagt ggcggttggc cataatgtgt catagccgtg cttggcgcgg ccacccacga
     3481 ctttgtctca acgagtgaaa gtcgtgcgtg gctagtacga ggctagccga ggcgcaagag
     3541 ggagaggccg cgcaatggca gctcatccgg cgcaaattaa acgacgcacg caatgacatg
     3601 ggcggcatca cccggcgtga agccgtcatt tgccagccg
//
LOCUS       DASWQN010000274         2183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1744056, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000274 DASWQN010000000
VERSION     DASWQN010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744056"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..553)
                     /locus_tag="VGC82_04555"
     CDS             complement(23..553)
                     /locus_tag="VGC82_04555"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEY0422575.1"
                     /translation="MGRSKHQAKHDLGRWLGGLLAVAVLFGLTEAASAQGAVRSVHGD
                     WQIRCDTPPGAQAEQCALIQSVVAEDRSNAGLTVIVLKTADQKSKLMRVVAPLGVLLP
                     SGLGLKLDNVDVGRAGFVRCLPNGCVAEVVMDEKLLGQLRTAKTATFIIFETPEEGIG
                     FPLSLNGIGEGYDKLP"
     gene            1047..1886
                     /gene="coxB"
                     /locus_tag="VGC82_04560"
     CDS             1047..1886
                     /gene="coxB"
                     /locus_tag="VGC82_04560"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY0422576.1"
                     /translation="MKMSKGQIGRRLLGLAVAGLALVAGGAAFAETGQPKPWEYTLQE
                     AASPVMENIISFHNLLLALITIITLFVLALLIIVMVKFNAKANPVPSKTTHNTLIEVA
                     WTLIPVLILVVIAVPSFRLLFQELDIPKPDVTIKATGKQWYWSYAYPDNGKFEFDSLL
                     AQDKQPRLLGVDNEMVVPVNKVIRVQVTAADVIHAFALPAFGVKIDAIPGRLNETWFK
                     ATQTGMFYGQCSELCGKDHAFMPIAIRVVSDQEFAAWAEAAKKKFATNPANSFASAGA
                     AAQ"
     gene            1962..>2183
                     /locus_tag="VGC82_04565"
     CDS             1962..>2183
                     /locus_tag="VGC82_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY0422577.1"
                     /translation="MATSATTHGDHAHDEHEHAHPTGWRRYVYSTNHKDIGTMYLVFA
                     MCAGLIGGFMSIAIRMELQFPGMQVFHDSH"
BASE COUNT          406 a          673 c          702 g          402 t
ORIGIN      
        1 tgcagcaaac cgggaggggg tctcacggca gcttgtcata gccctcgccg atgccgttca
       61 ggctcagcgg aaagccgatg ccctcttccg gggtctcgaa gatgatgaag gtcgcagtct
      121 tggcggtgcg gagctggccg agcagcttct cgtccatcac gacctcggcg acgcagccat
      181 tgggcaggca gcggacgaaa ccggcgcggc cgacgtcgac attgtcgagt ttgaggccca
      241 accccgaggg cagcagcaca ccgagcgggg cgaccacccg catcagcttg ctcttctgat
      301 cggcggtttt cagcacgatc acggtcaggc cggcgttgga acggtcttcg gctaccacgc
      361 tctggatcag ggcgcattgt tccgcctgcg cgcccggggg ggtgtcgcag cggatctgcc
      421 agtcgccgtg gaccgagcgc accgcgccct gcgcgctggc cgcctcggtc aggccgaaca
      481 gcacggccac ggcgagcagg ccgccgagcc agcggccaag atcgtgtttc gcctgatgtt
      541 tcgagcgccc catgcgggtc gatcctctga tattttccga cgccgccgga ctgataccca
      601 atgacggcgc cttaaaggcg gttcggccgc cgtccccgca cccgaaaggc cgttggcgcg
      661 ggcgaatcag gcatctcgga agcagcctgc ctgtgcctac atccggcaat ccgcctgtca
      721 agcgtagccg ggtggcaaag gcgcaggttc ggcctgattt agctggaaaa cctgacatcc
      781 agcgaatagc cggtgacgca tggcttggtg ccccactact gcattgcgag agccttggat
      841 ttatggtttg agaggctgga tttcgacgcg ttctaacgat ctggcccgag tactgacgca
      901 acggctgttg taggtacttt tgcaggatac ttctgaagcc gtacttctga agtagctccg
      961 gttggccgtt cgtcggggcg gatcgtgcgg catttccggc tatcggcggg ggtgctttgg
     1021 ggatttatca agggagcgcg agcggcatga agatgtcgaa gggccagatt ggccgtcggt
     1081 tgctgggatt agcggtggca ggcttggcgc ttgtcgctgg cggagcggca ttcgccgaaa
     1141 cgggccagcc gaagccgtgg gaatacacgc tgcaggaagc ggcttccccg gtgatggaaa
     1201 acatcatcag cttccataac ctcctgctcg cgctgatcac catcatcacg ttgttcgtgc
     1261 tggcgctgct gatcatcgtg atggtgaagt tcaacgccaa ggccaacccc gtcccctcca
     1321 agaccaccca caacacgctg atcgaggtcg cctggacgct gattccggtg ctgatcctgg
     1381 tcgtgatcgc ggtgccgtcg ttccggctct tgttccagga actggacatc ccgaagccgg
     1441 acgtgaccat caaggcgacc ggcaagcagt ggtactggag ctacgcctat cccgataacg
     1501 gcaagttcga attcgattcg ctgctggccc aggacaagca gccgcgcctg ctcggcgtcg
     1561 acaatgaaat ggtggttccg gtcaacaagg tgatccgggt tcaggtcacg gccgccgacg
     1621 tgatccacgc gttcgcgctg ccggccttcg gcgtcaagat cgacgccatc cccggccgtc
     1681 tcaacgagac ctggttcaag gccacccaga ccggcatgtt ctacggccag tgctcggaat
     1741 tgtgcggcaa ggaccacgcc ttcatgccga tcgcgatccg ggtggtgagc gatcaggaat
     1801 tcgcggcctg ggctgaggcg gcgaagaaga aatttgcgac caacccggcg aattcgttcg
     1861 cctcggctgg cgcagcggcg cagtaatcgc gccgccggct acgggacaag ggcgacggga
     1921 ccgcgaggtc cgacaaggga cttgctaggc aggatttgaa catggcaacg agcgcaacga
     1981 cacacggcga ccacgcacat gacgagcacg agcacgccca tccgaccgga tggcgtcgct
     2041 acgtctattc gaccaaccac aaggacatcg ggacgatgta cctggtgttc gccatgtgcg
     2101 cgggcctgat cggcggcttc atgtcgatcg ccatccgcat ggagctgcag ttccccggca
     2161 tgcaggtgtt ccacgacagc cac
//
LOCUS       DASWQN010000275         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2140055, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000275 DASWQN010000000
VERSION     DASWQN010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140055"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /locus_tag="VGC82_04570"
     CDS             complement(<1..1544)
                     /locus_tag="VGC82_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0422578.1"
                     /translation="MTDFIAYHGVSGATHQQHVDQLSVQGYRPVSISVSGNPLDARYA
                     AVWVHRPGPAWQAMHGISSAQYQARFDELVSQGYAPILVSATSFGGVEVFAAVFEQGV
                     TDPWFARHDLRWDPVTDPNTITHENQRAFDQGFIPRCLAVYGPSSDPRFAGIWVKNTA
                     PTPWSWWWTPPDAYQRFFDAELVAGLRPSYLSVASDGWLVSVFRDDQVGAWQARHNIP
                     APGYQAVFDTQVAAGLRPLLVQAGGTGNDARYAAIFAQDDAVTVRRWTVTGADFTGAS
                     DLDAAVRSFMTAHAIRAGTVAVARAGRVVANRGYTWAEPSYKITQPTTLFRIASLSKM
                     FTAAATARLVAQGRLAWNTKAFGFLGITTPPPANPAIDGVTVEQLVLRISGLARDFDQ
                     DLRGIAASIGRTTTPTLDDLIPYLYRQPLASTPGTTELYSNTAFFLLTAVVAKAAGRP
                     FIDEVRDGLLKPLGIDDVFLAATAPGGRRANEVAGYDHPGVKASQLDLSANALAPNAY
                     GGDFVV"
     gene            complement(1622..1777)
                     /locus_tag="VGC82_04575"
     CDS             complement(1622..1777)
                     /locus_tag="VGC82_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422579.1"
                     /translation="MSVSEALPLTVALLLAIVAAAMSSGPDTAQTISLYLTQALVPNI
                     VDLGAES"
     gene            complement(2080..2601)
                     /locus_tag="VGC82_04580"
     CDS             complement(2080..2601)
                     /locus_tag="VGC82_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422580.1"
                     /translation="MKYKISSAICMIQVRILRPARLCSEIGGNATMSMQRLLLIAAGL
                     GALATATPAGAQPNDSNISLNAPGLLIKKPPPGPPDVRTTPLAWPRLEPGAVLCRSEA
                     DLNRLARRRAGEATDGPVDCQTMRAPTAITIVQRAGPGRTEVKPTDPRVAASGWTDAW
                     LPEKAPANATAAR"
BASE COUNT          492 a          829 c         1056 g          534 t
ORIGIN      
        1 tcgaccacga agtcgccgcc atacgcgttt ggggccagtg cgttggcgct taggtcgagt
       61 tgcgacgctt ttacgccggg atggtcgtat ccggcgacct cattggcgcg gcggcccccg
      121 ggcgcagttg cggccaggaa aacgtcgtct atcccgagcg gcttgagcag gccgtcgcgc
      181 acctcgtcga tgaaggggcg gccggcggcc ttcgccacca ccgccgtcaa caggaaaaac
      241 gccgtgttcg agtacagctc ggttgttccc ggcgtggatg cgagcggctg gcggtaaaga
      301 tagggaatga gatcatccag cgtcggcgtc gtggtacggc cgatggaggc ggcgatgcct
      361 cgcagatcct ggtcgaagtc gcgcgccagg ccgctgatgc gcaggacgag ctgttccacg
      421 gtgacgccgt cgatcgccgg attggcgggt ggcggcgtgg tgatcccgag gaagccgaag
      481 gccttcgtgt tccaggcgag acggccctgg gcgaccagcc gggcagtggc agcggcggtg
      541 aacatcttgc tgaggcttgc gatgcggaac agcgtcgtcg gctgggtgat cttgtagctg
      601 ggttcggccc aggtatagcc gcgattggcc accacgcggc cggcgcgcgc aacggcgacg
      661 gtaccggcgc ggatcgcatg ggccgtcatg aaggagcgga cggctgcgtc cagatcgctc
      721 gcaccggtga aatccgctcc ggtcacggtc caacgccgaa cggttaccgc gtcgtcctgg
      781 gcgaagatcg cggcgtaacg ggcgtcgttg ccggtgccgc ctgcctggac caggagtggt
      841 cgcagtccag cggcgacttg ggtgtcgaac accgcctggt agccgggggc ggggatgttg
      901 tggcgggcct gccaggcgcc gacctggtcg tcgcggaaca ccgagacgag ccatccgtcg
      961 gatgcgaccg acaggtagga cggacgcagg ccggcgacga gttcggcgtc gaagaagcgc
     1021 tgatacgcgt cgggcggggt ccaccaccac gaccagggtg tgggcgcggt gttcttaacc
     1081 cagatgccgg cgaagcgcgg gtcggaggat ggcccataaa ctgccaggca gcgcgggatg
     1141 aagccttggt cgaacgccct ttggttctcg tgcgtgatgg tgttggggtc ggtgactggg
     1201 tcccaccgca ggtcatggcg ggcgaaccag gggtccgtca cgccttgttc gaagaccgcg
     1261 gcgaagacct cgacgccgcc gaagctggtg gcgctgacca ggatcggcgc gtagccctgg
     1321 ctgacgagtt cgtcgaagcg ggcttgatac tgtgccgacg agatgccgtg catcgcctgc
     1381 caggccggtc cggggcggtg tacccaaacg gcggcgtatc gagcatcgag cgggttgccg
     1441 gacacgctga tcgagaccgg gcggtatcct tggaccgaga gctgatcgac gtgctgctga
     1501 tgggttgcgc cggagacgcc gtggtaggcg atgaagtccg tcatcgtctt tgcctttgtg
     1561 ttgcagggcc tttgtgttgc aggcgttggc aggggccgga atggaccgag ccaaacgggg
     1621 atcaggattc ggcgccgaga tcgacgatgt tggggacgag ggcttgcgtc aggtagaggc
     1681 tgatcgtctg ggctgtgtcc ggaccggacg acatcgcggc agccacgatc gccagcaaga
     1741 gagcaacagt aagcgggagg gcttcggata cgctcatggc ggtttcccct tgttccgaga
     1801 cgacgttgga cgagggatta ggctggccac agggttggtc tgtgatctgg agcacattcg
     1861 tgctctccgg attggtttcg atgttggtgg cgtgagtggg acaggatgcg gcgtattata
     1921 aggaagctcg gcgggacgat gcggctggcc tacagcgccg ttgatggagg ggcgccatcg
     1981 gtatgaaggg caacaccgat gacgggggct gcggatcggc acgctgacgc ggcgctttaa
     2041 cctcggtcat gggggtgcgg catcagcgcg gacatcgatt taccgtgcgg cggttgcgtt
     2101 ggccggagct ttttccggta gccaggcgtc ggtccagccc gatgctgcaa cccggggatc
     2161 agtgggcttc acctcggttc ggccgggacc cgcacgctgc acgatggtga tcgctgttgg
     2221 ggcacgcatg gtttggcaat ccactggtcc gtccgtcgcc tcgccggcgc ggcgccgggc
     2281 gagccggttc aggtcggctt ccgatcggca caagaccgcc ccgggttcca ggcgcggcca
     2341 tgcaagcggc gttgtcctga cgtccggcgg tcccggcggc ggcttcttga tcagcagccc
     2401 gggggcgttg agggagatgt tgctgtcgtt aggctgggca ccggccgggg tggcggtggc
     2461 cagtgctccg agaccggcag cgatcaacag cagcctttgc atcgacatcg tcgcgttccc
     2521 accaatttcg gagcataacc gcgccggacg caatatccga acctggatca tgcagatagc
     2581 cgacgagatt ttatatttca tgctgcataa tcttccaagc acccggctgg ctgagacgct
     2641 agctggctaa aagtgatttt ctcaacggtg gcttcgtgag acagtcgcag caacaacacg
     2701 gatcacgctt ggccaagtgg cgacgtgccg gagtaacgtg ttcctggagc actgggaagc
     2761 tttgaccgag cggctctcgc cggtccgact acctgcggct tcgcggagtc atgcatgcaa
     2821 ggtttgattg tgcaaaggct gccgaagtcc aaatactttc tacctctggc cagataccat
     2881 atcatgtcaa gaatgacagt cgacgggcac c
//
LOCUS       DASWQN010000276         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2007878, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000276 DASWQN010000000
VERSION     DASWQN010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007878"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1028
                     /locus_tag="VGC82_04585"
     CDS             255..1028
                     /locus_tag="VGC82_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0422581.1"
                     /translation="MGIISLAGVDIDVWEGGDGPPLLFLHGAGGFRPGPAYLDLLGRH
                     RRIIAPSHPGFGRSGLADWMERPDDIAHLYLELLDRLDIGRLDIVGASFGGWIAAELA
                     TMQPQRLRRLVLVAPAGVKTGSRDELDIPDLFAQPADAVQRLLYVDPDKFRIDPAAMT
                     DEELRIMLRNRESFALFAWEPYLHNPKLPHRLHRVACPTLFLRGAHDGLISAEYTARY
                     AALLPDARVVTLPDAAHLPQLEQPEAFAAATLSFLQEEG"
     gene            1032..2216
                     /locus_tag="VGC82_04590"
     CDS             1032..2216
                     /locus_tag="VGC82_04590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0422582.1"
                     /translation="MRAWHFSETAYHLLPPAETYDSIRVSLPNKHYDPATGADLYHRY
                     LDEWLQAEDEGLDIMLNEHHQTATCVDPAAPLMLSILARQSSKARLLILGNPIANRRQ
                     PVRVAEEMAMIDVLSRGRLEAGFVRGVPYEISPANSNPVRMNEREWEALDMIIKAWTH
                     HDGPVSFEGRFFHSRAINIWPRPWQQPHPPIWISTTSPGGAAKVGEKGYVQATFLTGF
                     DGTKAIFDSYRSAWRAAGRGATVPLDRLAYAAMVYVGETEAHARAGAEKLLWYVTSNK
                     VPPHFASPPGYVPVETGLRMLRGAEHPLSAFAKAATVEGAIKAGIMMAGTPDQVVAQI
                     RAVYDHVGGFGHLLNMGQAGFLEHDETVAGIKMFAREVYPRLKAFPGVDVPGEHRGSA
                     SH"
BASE COUNT          402 a          756 c          725 g          394 t
ORIGIN      
        1 cgccggcgcg gcggacgtgg taggcctgac gtatacgttc gcgggcatgt tcttttcctt
       61 tacctcaagc gcctgtactt tacctcaagc gcctgtattt ccgaccggag taatgacgct
      121 aagaatccct gacaatactc cccgtcgccc gcggtgatca cggacatgcg gtcccctgag
      181 ccggcttgcg tgccttgcac catgacgggc tcaagcagat aggctagaat caatatcgag
      241 ggaaggaaac gaacatgggc atcatctccc tggccggggt cgacatcgat gtgtgggagg
      301 gtggggacgg tccaccgctg ctgttcctgc acggcgccgg cggcttccgc cccggccccg
      361 cctacctcga cctgctcgga cggcatcgca ggatcattgc gccctcgcat cccggattcg
      421 gtcgatcggg cttggcggac tggatggagc ggcccgacga catcgcccat ctgtatctgg
      481 agctgctcga ccggctggac atcggcaggc tcgacatcgt gggtgcatcg tttggcggct
      541 ggatcgcggc tgaactcgcg accatgcagc cgcaacggct gcggcggctg gtgctggttg
      601 ccccggctgg ggtgaagaca ggcagccgcg acgagctcga cattccggac ctgttcgccc
      661 agccggcgga cgccgtgcag cgcctgctgt atgtggaccc cgacaagttc cgcatcgacc
      721 cagccgccat gaccgacgag gaactgcgga tcatgctgcg caaccgggaa tccttcgcgc
      781 tattcgcgtg ggaaccgtac ttgcacaacc ccaaactgcc gcaccgtctg caccgggtgg
      841 cctgccccac gctgttcctg cgcggcgcgc acgacgggtt gatctcggct gaatataccg
      901 cccgctacgc tgccctgctg cccgacgcgc gtgtcgtcac gctgcccgat gccgcccacc
      961 tgccgcaact ggagcagccc gaagccttcg ccgccgcaac gctatcattc ctgcaagagg
     1021 agggctgacc gatgcgcgcc tggcatttca gcgagaccgc ctatcatctg ctgcccccgg
     1081 ccgagaccta cgactcgatc cgggtcagcc tgcccaacaa gcactacgac cccgcgaccg
     1141 gcgccgacct ttatcaccgc tacctggacg agtggctgca ggcggaggac gaaggcctgg
     1201 acatcatgct gaacgagcat caccagaccg cgacctgcgt cgatccggct gccccgctga
     1261 tgctgtcgat cctggcgcgc caaagctcca aggccagatt gctgatcctc ggcaacccga
     1321 tcgccaaccg ccgccagccg gtgcgcgtcg ccgaggaaat ggcgatgatc gacgttcttt
     1381 cgcgcggacg gctggaggcc gggttcgtgc gtggcgtgcc ttacgaaata tctccagcca
     1441 acagcaatcc cgtgcggatg aacgagcgcg agtgggaagc gctggacatg atcatcaagg
     1501 cctggacgca ccacgatggt ccggtcagct tcgagggtcg tttcttccac agccgagcga
     1561 tcaatatctg gccgcgtccg tggcagcagc cgcacccgcc gatctggatc agcaccacca
     1621 gccccggcgg cgctgccaag gtgggggaaa agggctacgt gcaggcgacg ttcctgaccg
     1681 gcttcgacgg caccaaggca atcttcgact cctatcgttc ggcatggcgc gccgcgggcc
     1741 gtggcgctac cgtgccgctc gaccggctgg cctatgcggc catggtctat gtcggcgaaa
     1801 ccgaggcgca tgcgcgcgcc ggggccgaaa agctgctgtg gtacgtcacg tcgaacaagg
     1861 tgccgccgca ctttgccagc ccgccgggct atgtcccggt cgaaaccggc ttgcgcatgc
     1921 tgcgcggtgc cgaacatccg ctgagtgctt tcgccaaggc ggcgacggtg gagggggcga
     1981 tcaaggccgg catcatgatg gccggcacac cagatcaggt cgtagcacag atccgggcgg
     2041 tgtacgacca tgtcggaggc ttcgggcacc tgctgaacat ggggcaggcc ggattcctgg
     2101 agcatgacga gaccgtggcc ggcatcaaga tgttcgcgcg cgaggtctat ccacggctga
     2161 aggcgttccc tggcgtggac gttcctggcg aacatagagg atcagcaagc cactgatgcg
     2221 ggcaccggag aacgaatggc cttcggaact caggcttaga ccgttttccg ttcgcct
//
LOCUS       DASWQN010000277         3957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_951919, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000277 DASWQN010000000
VERSION     DASWQN010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951919"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VGC82_04595"
     CDS             <1..191
                     /locus_tag="VGC82_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422583.1"
                     /translation="VGGNIAGYTRTMTTAITLETSQGNFAFALALGAVLVTISIVVSV
                     CAFGIEAWSRRTSSKETA"
     gene            188..1003
                     /locus_tag="VGC82_04600"
     CDS             188..1003
                     /locus_tag="VGC82_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0422584.1"
                     /translation="MKTAGTLCGMLLLLGLLPGQAAEPPAIVLASTTSVQDSGLLTRI
                     LPRFTGETGIQVRVLAQGSGQALATAARGDADLVLVHDPDAEAAFMAAGHGISRTEIA
                     WNDFVLVGPEADPAHVAGRPDATEALKAIAAASVPFVSRGDKSGTDAEEKRLWRAAGI
                     LPSGAWYRDIGGGMGAALNAASAMGAYTLSDRGTWLSYGNKVGMKIVLQGDRRLLNLY
                     DVILLNPRTHPDAKQAPARRLADWLGSPEGQTAIGSYTKDGQRLFHPEADPKP"
     gene            complement(1178..2521)
                     /locus_tag="VGC82_04605"
     CDS             complement(1178..2521)
                     /locus_tag="VGC82_04605"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEY0422585.1"
                     /translation="MSGSNLFGEIRRFARTSGAVGGIAARVAGERMFGIKTDRAAHAE
                     DLRTILGGLKGPLMKVAQFLSTVPDALPAEYTAELAQLQANAPAMGWAFVRRRMASEL
                     GPNWQSKFASFSQEASAAASLGQVHRARLPDGTDVACKLQYPDMPSTVEADLRQLRLA
                     MAVYHRMDNAIQQDEIYKELRERLREELDYLREASQMRLYGLMLRDQPGVHVPVPLPE
                     FSTNRLLTMTWLDGRPLMDRLAEDPPQDERNKIAEALFRAWYVPFYQFGVIHGDPHLG
                     NYQVRQDGSVNLLDFGAIRVFNPRFVSGVIELFEAVRDHDDDKAHHAYQTWGFTDLSR
                     EKMAVLNMWARFLYEPLIQDRVRRIQETNDPTFGREVAERVHAGLKRTGGVMPPREFV
                     LMDRSAIGLGGVFLRLKAELNWSRLFSDLIADFNEAALAERQASALSEAGVPVAR"
     gene            complement(2648..3598)
                     /locus_tag="VGC82_04610"
     CDS             complement(2648..3598)
                     /locus_tag="VGC82_04610"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0422586.1"
                     /translation="MKAGFIGLGTMGASMAANLQKGVQKDGHTVLVHDVRREAASAHI
                     RNGAVWADSPALIAAECDIIFSSLPGPVEFEAVSYGDSGLLATIRPGAAYFDLTTNSP
                     TTVRKAHAEYEKKGAYLFDAPVSGGPRGAASGQLAIWCGGDAEMFEKWKPVLDTIGDA
                     ARYIGPIGAGSVAKLVHNCYGYIATAAAAEVFSMGVKAGIEPLAVWEAVRQGAIGRRG
                     AFESMTDQFLPDKYEPAAFALRLAHKDVSLATGLGRELNVPMRLSNLALADLAEGLNR
                     GWGNRDSRSIMALQTERAGVEIKVDPQRLAEAIENKTKKA"
     gene            complement(3630..>3957)
                     /locus_tag="VGC82_04615"
     CDS             complement(3630..>3957)
                     /locus_tag="VGC82_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0422587.1"
                     /translation="MAQAPSSASRDRVRDTVPKLIEVTEKVLFGDIWERPELSKRDRS
                     LIVCAVLTATYRPEQLRGHLQRALDNGLTKSEISEMITHVAFYAGWPASMSAANIARE
                     IFEANP"
BASE COUNT          705 a         1316 c         1218 g          718 t
ORIGIN      
        1 tcgtcggagg caacatcgcc ggttataccc gcacgatgac gacggccatc acgcttgaga
       61 ccagccaggg gaacttcgcc ttcgcgctgg cgctgggcgc ggtgttggtc acgatcagca
      121 tcgtggtcag cgtctgtgcc ttcgggatcg aggcctggtc gcggcgaaca tcctcgaagg
      181 agaccgcatg aagacggctg gcacgctgtg cggaatgttg ctcctgctcg ggttgcttcc
      241 ggggcaggca gcggagccgc cggcgatcgt cctggcctcc accacctcgg ttcaggatag
      301 cggattgctg acccgcatcc tgccgcggtt taccggcgaa accggcatcc aggtgcgcgt
      361 gcttgcccag ggcagtggcc aggcgctggc tactgccgcc cgtggcgatg ccgacctggt
      421 gctggtgcac gaccccgacg cggaggcggc cttcatggcg gccggtcacg gcatcagccg
      481 gaccgagatc gcctggaacg atttcgttct ggtcgggcct gaagcggacc ccgcgcatgt
      541 cgcgggtcgt cccgatgcca ccgaggcgct gaaggcgatc gccgctgcaa gcgtgccgtt
      601 cgtatcgcgc ggcgacaaga gcggcacgga tgccgaggaa aaacgtttgt ggcgggccgc
      661 gggaatcctc ccgtcaggcg cgtggtatcg cgatatcggc ggcggcatgg gggccgcgct
      721 caatgccgcg agtgcgatgg gcgcctatac gctgtcagat cgcgggacat ggcttagcta
      781 cggcaacaag gtaggaatga agatcgtgct gcagggcgac cgccgcctgc tgaacctgta
      841 cgacgtcata ctgctgaacc cgcggactca tcccgatgcc aagcaggctc cggcgcgtcg
      901 ccttgcggat tggctgggct cgccagaggg gcagacggcg atcggcagct acacgaagga
      961 cgggcagagg ctttttcatc cggaagccga tccgaagcca tgaacatttc cggcccggac
     1021 gcaagcctgc cgtacccggc ttgttgggct gtcttcgagc cgggcaaacc gccccagcaa
     1081 tgtcatgatc gacgcaggct cgcaacgaca ggcccgcaac gacagtatcg aaggccgcgc
     1141 tcggagcggt tatttgccag caaactggcg ggacgcgcta ccgggccacg gggaccccgg
     1201 cttcgctcag tgccgacgcc tgacgttcgg ccaacgctgc ttcgttgaag tcggcgatca
     1261 ggtcgctgaa cagccggctc cagttcagtt ccgccttcag gcgcaggaac acaccgccca
     1321 ggccgatggc cgaacggtcc atcagcacga attcgcgcgg cggcatcacc ccgccggtgc
     1381 gcttcaatcc ggcatgaaca cgttcggcta cttcacgccc gaaagtaggg tcgttcgtct
     1441 cctgaatgcg ccgcacccga tcctgtatca ggggctcata aagaaaccgc gcccacatgt
     1501 tcagcacggc cattttttca cgcgacagat cggtgaaacc ccaagtctgg taagcatgat
     1561 gcgccttgtc gtcatcatga tcgcgcacgg cttcgaacag ctcgatcacg ccgctcacga
     1621 agcgcgggtt gaatacccgg atagccccga agtcgagcag gttgaccgac ccgtcctgcc
     1681 gcacctgata atttcccaga tgcgggtcac cgtgaatcac accgaactgg tagaacggca
     1741 cataccaggc tcggaacagc gcctcggcga tcttgttgcg ttcgtcctgc ggcggatcct
     1801 cggcgaggcg gtccatcagc ggccgaccgt ccagccaggt catggtcagc aacctgtttg
     1861 tactgaactc gggcaaaggg accggcacat gcacacccgg ttgatccctt agcatcaagc
     1921 cgtaaagtcg catctgcgag gcctcgcgaa ggtaatccag ttcctcccga agacgttcgc
     1981 gcagttcctt gtagatctcg tcctgctgga tggcgttgtc catacggtga tacaccgcca
     2041 tcgccagtct cagttgccta aggtcggcct caacggtgct gggcatatcc ggatactgca
     2101 gcttgcaggc aacgtccgta ccgtccggca acctggcgcg atgcacctgc ccgaggctgg
     2161 cagccgccga tgcctcctgg ctgaacgacg cgaatttcga ttgccagttc ggcccaagtt
     2221 cgctcgccat gcggcggcgg acgaaagccc agcccatggc cggcgcgttg gcttgaagct
     2281 gcgccagttc cgccgtgtat tccgccggaa gtgcgtcagg gacggtggaa aggaactgcg
     2341 ccaccttcat cagcggcccc ttcaggccac cgaggatggt cctcagatcc tcggcgtgcg
     2401 ccgctcggtc ggtcttgatc ccgaacatcc gctcaccggc cacacgggcg gcgataccgc
     2461 cgacggcacc ggatgtgcga gcgaagcgcc tgatttcgcc gaacaggtta ctaccggaca
     2521 tcgcggcgcc acggctgcgt atcaatcggc ttcatcgtgt tgatctcgtt cggcgtcgca
     2581 tcaaccttca tatctgcggt gccgccctga ttgcaacccg cttctgctca acggaacggc
     2641 caatttccta ggctttcttc gtcttgttct cgatcgcctc ggccaggcgc tggggatcga
     2701 ccttgatctc gacgcccgcc cgttcggtct gcaacgccat gatcgaccgc gagtcgcggt
     2761 tgccccagcc gcggttcaat ccttccgcca gatcggccag agccaggttg gacagccgca
     2821 tcggaacgtt cagttcacgg cccaagccgg ttgccaggga gacgtccttg tgcgccaacc
     2881 gcaacgcgaa agcggccggc tcatacttgt ccggcagaaa ctggtcggtc atggactcga
     2941 atgccccgcg ccggccgatg gccccctggc ggacggcttc ccacaccgcc agcggctcga
     3001 tgcccgcctt gacgcccatg ctgaacacct cggcagccgc ggctgtcgcg atgtagccgt
     3061 agcagttgtg caccagcttc gcgaccgatc cggcgccgat cgggccgatg tatctcgcag
     3121 cgtcgccgat ggtatccagc accggcttcc acttctcgaa catttcggcg tcaccgccgc
     3181 accagatcgc cagttggccg gacgccgcac cgcgcgggcc gccactgacc ggcgcgtcga
     3241 acaggtaggc gcccttcttc tcatactccg catgggcctt gcgaacggtt gtcggcgagt
     3301 tggtcgtcag atcgaaatac gccgcacccg gacggatcgt ggccagcaag ccggagtcgc
     3361 catacgagac cgcttcgaac tcgacggggc ccggcaggga cgaaaagatg atgtcgcact
     3421 cggctgcgat caaagccggg ctgtccgccc agaccgcgcc attcctgata tgcgccgaag
     3481 cggcttcccg cctgacgtcg tgtaccagga cggtgtgacc gtccttctgc acacccttct
     3541 gtagatttgc ggccatgctg gcacccatgg tgccaaggcc gataaagccc gccttcatcg
     3601 tttccgtccc cgcctgaaca cgctcaacct tatggattgg cctcaaagat ctcgcgggcg
     3661 atgttcgccg ccgacatcga tgccggccag ccggcataga acgccacgtg cgtgatcatc
     3721 tcggagattt ccgacttggt caggccgttg tccaacgccc gctgcaaatg gccgcgcagt
     3781 tgctccggcc gataggtcgc ggtcagcacc gcgcaaacga tcaggctgcg atcgcgcttc
     3841 gacagttccg ggcgttccca gatatcgccg aacaggacct tttcggtcac ctcgatcaac
     3901 ttagggacgg tatcccggac gcggtcgcgc gatgcggacg atggggcttg tgccatg
//
LOCUS       DASWQN010000278         3983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1348195, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000278 DASWQN010000000
VERSION     DASWQN010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348195"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..673
                     /locus_tag="VGC82_04620"
     CDS             182..673
                     /locus_tag="VGC82_04620"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0422588.1"
                     /translation="MKTLLIVFHSLTGGTRQMAEAAAMGAAEEPDVNVRLVAALDAEA
                     SDVLAADGYVFATPENLAAMAGRMKDFFDRTYYPALDRINGRPYVSLVCAGSDGENAV
                     RQIARIATGWRLRPIADALIICTHAQTPEAIMAPKTIGPADLGRCHDLGAALSHGLAM
                     GIF"
     gene            777..1208
                     /locus_tag="VGC82_04625"
     CDS             777..1208
                     /locus_tag="VGC82_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422589.1"
                     /translation="MEHDGGCHCGNLQVRVRLTKPPAENTVRSCACSFCRAHATRTVS
                     DPAGHADIRAVDWSLVEKYRFGSRTADYLLCRRCGVYIGAVCSTAAGVRLVINTHCLG
                     DRSAFTQDPVRPDYDTEATEARLARRAANWMPAMLHEAARS"
     gene            1321..2175
                     /locus_tag="VGC82_04630"
     CDS             1321..2175
                     /locus_tag="VGC82_04630"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY0422590.1"
                     /translation="MEFEQIEYEVKGTTAIVTLNRPERRNALSGRLLRELNAALKEAD
                     EYNPVHCVVLRGAGPHFCSGADLSEYSTGRGPDYRGRVEIDDDIWRLEQGQRLRIELF
                     DMHKPVIAQVHGTCIGIGTELAWFCDMIIVADDARIGFPPVRDLGTPPGSMWLYHCGP
                     QWAKRLLLTGDSLSGKDAAEIGLALKSYPPAELETEVMRLADRMGLVDPHLLSANKRM
                     VNLGLELMGARTIQRIGAEIDARGHLAPKAMEFRATMREHGVKHAFQKRDSAFGSGYA
                     AVRGRDPD"
     gene            complement(2251..3147)
                     /locus_tag="VGC82_04635"
     CDS             complement(2251..3147)
                     /locus_tag="VGC82_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3445 domain-containing protein"
                     /protein_id="HEY0422591.1"
                     /translation="MPRQVVHRPFESGLYRMAMDLVTVSEAAWFEFDDRYVPEMGEKR
                     HLLASAHHEVFAAMADSSAARGEALELIVAALTAHHPDWFSRDGDRLHNHLTGEFHAA
                     SAGDRLELAARLVQEDLCLIQNDAAGPVLTAAALCFPSRWRLLDKIGLPLAAVHGPVP
                     FYADRLAGAVDRFMRHLKPGHIVSRLNWSLLDDPALFQPGGKWRIGDSTEITANNAGS
                     RVFLRVERQTLRRLPVTGAVLFGIRVHVYPLDRVIDQPEHAATLAEAVRSLPQEMQHY
                     KSLLPFREPLLEWLGIAGANMA"
     gene            complement(3161..3982)
                     /locus_tag="VGC82_04640"
     CDS             complement(3161..3982)
                     /locus_tag="VGC82_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0422592.1"
                     /translation="MAGKFRKLALREWGDADAPVVVCVHGLTRNGHDFDALARDRAGS
                     YRVICPDLPGRGHSDWLDDPMLYQAQHYVTALAHLLAWIGRDVAWVGTSLGGICGMVV
                     AATDGAPISRLVLNDVGPFIPADAQRRIRDYMLASGASPAMSRFPDIEAVERHLRLVH
                     APFGPLSDAQWAELARNSARTLPDGRFTMHYDPRIGEPLRGHDPVDVDMWAFWDKISV
                     PRLVIRGETSDILLPETLARMAISGAEVHVVAETGHAPALLDPAQIEAIRSFLAS"
BASE COUNT          731 a         1306 c         1271 g          675 t
ORIGIN      
        1 gctggcgtcg cgaccgtcgc cctgacaacc atgacccaca ccaccatgac gatgctgttt
       61 ccggcactgc ccgaggcatt gcgagacctg aacatcggca tcgtcgcgct ggtcttgaat
      121 ctggtgacgc tggcaatcgt cagcgccgtt acctaccgcc gggcagcgta cgcatgacgg
      181 gatgaaaacc ctgctgatcg tcttccattc gctgaccggc ggcacaaggc agatggccga
      241 ggcggcggca atgggggcgg cggaggagcc ggatgtcaac gtgcgcctgg tggccgcgtt
      301 ggacgccgaa gcgtcggatg ttcttgccgc cgatgggtat gtcttcgcga cccccgagaa
      361 cctggcagcg atggccgggc gcatgaagga tttcttcgac cgcacctact atccggcgtt
      421 ggaccggatc aacggccggc cctacgtctc gttggtatgt gccggcagcg acggagaaaa
      481 cgcagtccgg cagatcgccc gtatcgccac cggatggcgc ctgcgtccca tcgccgatgc
      541 gctgatcata tgcactcatg cgcagacgcc ggaggcaatc atggcgccga agacgatcgg
      601 cccggccgat ctcggccgct gtcatgatct cggcgccgcg ctgtcccacg gtctcgccat
      661 gggcattttc tagcgatcct tggtcctgtt tcgcctgtac aatctagctg aaacgctata
      721 gccagacagg gtctcctgtt ccaaaatccg cggcttacgc atgctggagg acagcgatgg
      781 aacacgatgg tgggtgccac tgcggcaatc ttcaggttcg cgtgcggctg acaaagccgc
      841 cggccgaaaa cacggttcgc tcctgcgctt gttcgttctg tcgagcccat gcgacccgga
      901 ccgtgtcgga tccggccgga catgcggata tccgcgcggt tgattggtct ctggtggaga
      961 agtatcggtt cggatctcgc accgcggact acctgctgtg ccgccggtgc ggtgtgtata
     1021 tcggtgcggt gtgcagcacg gccgccggcg ttcgtttggt catcaacacg cactgtctgg
     1081 gtgaccgatc agcatttacc caggatccgg tgcgccccga ctacgatacc gaagcgacag
     1141 aggcgcgcct cgctcgtcgt gcagccaact ggatgccggc gatgctgcat gaggcggcaa
     1201 ggtcatagca cggccgcggc atgccgaacg gcgaacctcg atgggtatcg ttgcgagggc
     1261 cgcgctcttg cctccgccgc acacagtgcg tttgatccag gtcagcacaa ggagaccagc
     1321 atggaattcg agcagatcga gtacgaggtc aaaggcacca cggccatcgt cacgttgaac
     1381 cgcccggagc ggcgcaacgc gctcagcggc aggctgttgc gcgaactgaa cgcggcactg
     1441 aaggaggcgg acgaatacaa tccggtccac tgcgtcgtgc ttcgcggtgc cggtccgcat
     1501 ttctgttccg gagcggattt gagcgaatac tccacgggcc gaggcccgga ttatcgcggc
     1561 cgcgtcgaga tcgacgacga catctggcgg ctggagcagg gtcagcggct gcgcatcgag
     1621 ctgttcgata tgcacaagcc ggtgatcgcg caggtgcatg ggacatgcat cgggataggc
     1681 acggaactcg cctggttctg cgacatgatc atcgttgccg acgacgcccg cattggcttc
     1741 ccgccggtcc gcgacctggg cacgccaccc ggcagcatgt ggctgtatca ttgcggcccg
     1801 caatgggcga agcgcctgct gctgacgggc gattcgctga gcggcaaaga tgccgcggag
     1861 atcggtctgg cgctgaaatc ctatccgccg gcggagcttg aaaccgaggt catgcgcctg
     1921 gcggaccgca tgggtctggt ggacccgcat ctgctctccg ccaacaaacg aatggtcaat
     1981 ctgggcctgg agctgatggg tgcacgcacg atccagcgca tcggtgccga gatcgacgcc
     2041 cgcggtcacc tcgcgcccaa ggcaatggag ttcagggcaa cgatgcgcga acacggggtg
     2101 aagcacgcat tccagaagcg cgattccgcg ttcggaagcg ggtacgctgc ggtacgcggg
     2161 cgcgatccgg attaagccgc gagcgggccc gcctcgcgcg gccatgttcc tactcatgga
     2221 gccgtactgc ggaagcagca ggcagcggtc tcaggccatg ttggcgcccg caattcccag
     2281 ccattccagc aacggctctc gaaacggcag caggcttttg tagtgctgca tctcctgcgg
     2341 caatgatcgc accgcttcgg ccagggttgc cgcgtgttcg ggttggtcga tcacccggtc
     2401 caacgggtaa acgtgcacgc gaatcccgaa tagcaccgcc ccggtcaccg gcagccgccg
     2461 cagtgtttgc cgctccaccc gcaggaacac ccggctgccc gcgttgttcg cggtgatctc
     2521 cgtcgaatcg ccgatccgcc acttgccgcc gggttggaac aatgccgggt cgtccagcag
     2581 cgaccagttt aaccgagaca cgatgtggcc aggcttcagg tgccgcatga accggtccac
     2641 cgccccggcg aggcggtcgg catagaacgg cactgggcca tgcacagccg ccaacggcag
     2701 cccgatcttg tccagcagcc gccagcgact gggaaagcac agggcagcgg cggtcagcac
     2761 cggccccgca gcatcattct gaatcaggca caggtcttcc tgcaccagcc gcgccgccaa
     2821 ctccagtctg tcgccggcgg aagcggcgtg gaattccccg gtgaggtgat tgtgcagccg
     2881 atccccgtcc cggctgaacc agtccgggtg atgcgcggtc agcgccgcga cgatcaactc
     2941 cagcgcctcg ccgcgggcgg cagacgaatc cgccatggca gcgaagacct cgtggtgcgc
     3001 gctagccagc aggtgacgtt tttcccccat ctccggcacg tagcggtcat cgaactcgaa
     3061 ccacgccgct tcggataccg tgaccagatc catcgccatc cggtagagcc cggactcgaa
     3121 cggccgatgc accacctgtc gcggcaacga ccgcatctcg tcagcttgcc aggaacgacc
     3181 ggatagcctc gatctgcgcc ggatccagca gcgccggggc atgaccggtt tccgccacca
     3241 catggacctc ggcaccagag attgccatgc gggcgagcgt ttccggcaac aggatgtcgc
     3301 tggtctcgcc ccggatcacc agccgcggca ccgagatttt gtcccagaat gcccacatat
     3361 ccacatccac ggggtcgtgc ccgcgcagcg gttcgccgat acgcgggtcg taatgcatgg
     3421 tgaaccggcc gtccggcaac gttcgggcgg agttgcgggc aagttccgcc cactgcgcat
     3481 cggatagcgg cccgaatggc gcgtggacca gtcgcagatg ccgttcgacc gcttcgatat
     3541 ccggaaaacg cgacatcgcc ggcgaagcgc ccgatgccag catgtagtcg cggatacgcc
     3601 gctgcgcgtc cgccggaatg aagggaccga cgtcgttcag caccagacgg ctgatgggcg
     3661 cgccgtcggt cgcggcgacc accataccgc agatgccgcc cagcgaagtg ccgacccaag
     3721 ccacatcgcg gccgatccag gccagcagat gcgccagcgc cgtcacgtaa tgctgtgcct
     3781 ggtacagcat cgggtcgtcc agccaatcgg agtgtccgcg tcccggcaga tccggacaga
     3841 tcactcggta tgaaccggca cgatcacggg ccaaggcatc gaagtcgtgc ccgttgcggg
     3901 taagcccgtg cacgcagaca acgaccggag cgtcggcgtc gccccattcc ctcaatgcca
     3961 atttgcgaaa tttgcctgcc agc
//
LOCUS       DASWQN010000279         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_688224, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000279 DASWQN010000000
VERSION     DASWQN010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688224"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..416
                     /locus_tag="VGC82_04645"
     CDS             141..416
                     /locus_tag="VGC82_04645"
                     /inference="COORDINATES: protein motif:HMM:NF023665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEY0422593.1"
                     /translation="MPLIPSQDSPMQRIRHPPLRNKIDLAEPTQLRAWTRRLGMSADA
                     LKAVVDKVGNSVAAVTKEIELRRASPQASPATVQSPSAEGELPKAAC"
     gene            complement(516..1001)
                     /locus_tag="VGC82_04650"
     CDS             complement(516..1001)
                     /locus_tag="VGC82_04650"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY0422594.1"
                     /translation="MKLKYLAAALLGTALLTGAAFAETATNDRSSVNTAVHRDGQWRA
                     SKLIGVNVYNEANEKIGEIEELIVDKSGKVDNVVLGVGGFLGMGEHYVAVPMEKLKWV
                     NEPVRTSSTTTAPADKSTVGEANRANRPQRAADEKWYPDHAVFNATKDQLKAMPQFKY
                     N"
     gene            complement(1167..>1698)
                     /locus_tag="VGC82_04655"
     CDS             complement(1167..>1698)
                     /locus_tag="VGC82_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422595.1"
                     /translation="RDTAHGFLAWALASVVGAILLASPATSLIGGATSGAVQASASQA
                     GPMDSYVDTLLRPNNPTADNAPNANGGELRRELVRLFTADFRNGGEPSAADRSYIAKV
                     VAARTGLSQADADKRVSDVIVQVKADLDKARKAAMKLAIWLTLSLFVGAFSAALAATE
                     GGGLRDGTWGKTALGR"
BASE COUNT          347 a          506 c          490 g          355 t
ORIGIN      
        1 cacgactgag cttcgcgaac cacaattggc ttgaatcgtc catcagacga caggcccgtt
       61 tttcgcgcgg cccttgtcgt atcaggaggt cttgatctta tgaggagacg tcacagccga
      121 aatggaacga aggtcttggg atgccgttga tccccagtca ggacagcccc atgcaacgca
      181 tcagacatcc gccccttcgc aacaagatcg atttggcaga gccaactcag cttcgtgcct
      241 ggacccggcg gctcggaatg tccgctgatg cattgaaggc cgttgtcgac aaggtgggta
      301 actccgtcgc ggccgtgacc aaggaaattg aacttcggcg ggcaagtccc caggcatcgc
      361 cggcgacggt ccaaagccca tctgccgaag gcgaattgcc gaaggcagct tgttgacagt
      421 gacgcgcctg gctcgccagc ggcgtgaccc tatcgcagca ttcgcaaaac aaggccgccc
      481 cgccgacatg gcggggcggc ttctttgttc gctggttagt tatatttaaa ctgcggcatc
      541 gccttcagct ggtccttggt cgcgttgaag acggcgtgat ccggatacca cttctcgtcc
      601 gccgcacgct gaggccgatt ggcacgattg gcttccccaa ccgtggattt gtcggccggc
      661 gcggtggttg tcgatgaagt gcgaacgggt tcattgaccc atttcaactt ctccatcggc
      721 accgcaacgt agtgttcgcc catgccgaga aagccgccga cccccaagac gacgttatcg
      781 accttgccgg acttgtcgac gatcaactcc tcaatctccc cgatcttctc gttggcttcg
      841 ttatacacgt taacgccgat caacttggaa gccctccact ggccgtccct gtggacggcc
      901 gtgttgacgc tggagcgatc gttagtggcg gtctccgcga aggccgcacc ggtcaaaagc
      961 gcggtgccga gcaaggctgc cgccagatat ttcaatttca ttttttagtc tccagtaaga
     1021 ttcgagttga ggggtgattg tttttggaca ccaaatcaaa ctgtcagtgg tgcagcagca
     1081 tgatcagaat gatcagcggg atcggtattc caagcagcca cagaagaatt ggcatgagta
     1141 tctcctttcg gtttggttga tgagggttaa cgacctagtg ctgtcttgcc ccatgtaccg
     1201 tcgcgaaggc ctccgccctc ggtcgctgcc agcgctgccg agaaagcacc gacgaaaagc
     1261 gaaagcgtca gccagatggc cagcttcatc gcggccttgc gggctttatc gagatcggcc
     1321 ttgacctgaa cgatcacatc gctgacgcgc ttgtccgcgt cggcctggct aagtccggtt
     1381 cgggcagcga ccactttcgc gatatagctg cggtcggccg cagaaggttc gccaccattg
     1441 cggaagtcgg cggtaaacag gcgaaccagt tcccggcgca attccccgcc attggcattc
     1501 ggcgcgttgt ctgcagtagg gttgttcggc cgcaacaagg tatcgacata gctatccatc
     1561 ggacccgcct gcgaagcgct ggcctgcacc gcgcccgacg ttgcaccgcc aatcaacgag
     1621 gtggcgggag aggctagaag gattgcgccg accacggaag ccaatgccca cgccaggaaa
     1681 ccatgtgcgg tatcacga
//
LOCUS       DASWQN010000280         1741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_292789, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000280 DASWQN010000000
VERSION     DASWQN010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292789"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..>1741)
                     /gene="purL"
                     /locus_tag="VGC82_04660"
     CDS             complement(11..>1741)
                     /gene="purL"
                     /locus_tag="VGC82_04660"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY0422596.1"
                     /translation="VGVPTVGGEINFHHAYDGNPLVNAMTVGIARKDRIFLSAAAGIG
                     NPVVYVGSKTGRDGIHGATMASAEFAADTEEKRPTVQVGDPFTEKLLIEACLELMATD
                     AIVAIQDMGAAGLTSSSVEMAGKGNVGIELDLDQVPARETGMTAYEFMLSESQERMLM
                     VLRPDREQLARDIFAKWDLDFAVIGRITDTGRIVVRHHGIVEADIPLAPLNDQAPLYT
                     RPTVDTPKSEKLNPARIEDRYGLTRALEKLVGCPDLCSRAWVWDQFDSTVGGQTVKRP
                     GAADAAVVKLEGLKRALALTIDCTPRYCLADPESGGAQAVAEAWRNLTAVGARPLAIT
                     DNMNYGNPEKPEIMGQFAAAIRGMAAACEALDFPVVSGNVSLYNETEGRAILPTPAIG
                     GLGVLDDASRAVGLALPPWLDIVLVGATIGWLGQSMWLREIAGREDGAPPPVDLAVEK
                     ANGDFVRTHILNGTIRACHDVSDGGVLVALAEMAMASGIGARMMTHPRDIPGHAFWFG
                     EDQARYLVAVPDHVAFTRIAEAAGIPAMRLGTSGGQDLTLPDGGTISINALRTAHEHF
                     FPSWMNGPTS"
BASE COUNT          283 a          553 c          593 g          312 t
ORIGIN      
        1 tggacgtagt ctagctcgtg ggcccgttca tccaggacgg gaagaaatgt tcgtgcgccg
       61 tccgtaacgc attaatggat attgtgccgc cgtccggcag agtcaaatcc tggccacccg
      121 aggtgcccag tcgcatggca gggatgccgg cagcttcggc gatacgggta aaggccacgt
      181 ggtccggcac cgccacgagg tagcgggcct ggtcctcgcc gaaccagaaa gcgtggccgg
      241 ggatgtcgcg cgggtgggtc atcatgcggg cgccgatgcc gcttgccatc gccatttcgg
      301 ccagcgcgac cagcaccccg ccatccgaca cgtcatggca cgcgcggatc gtgccgttca
      361 ggatgtgggt gcggacgaaa tcgccgttgg ctttttccac cgccagatcg accggcgggg
      421 gagcaccgtc ttcgcggccg gcgatctcgc gcaaccacat cgactgcccg agccagccaa
      481 tggtcgcgcc gaccaggacg atgtcgagcc acgggggcaa cgcaagtccg actgcgcgcg
      541 atgcgtcgtc caggacacca agcccgccga tcgccggggt gggcaggatc gcgcgaccct
      601 cggtctcgtt gtacagactg acgttgccgg atacgacggg gaagtcgagg gcctcacagg
      661 cggcagccat cccgcggatc gccgcggcga actggcccat gatttccggc ttttcgggat
      721 tgccgtaatt catgttgtcg gtgatcgcca atggccgggc accgacggcg gtcaggttgc
      781 gccaggcttc ggcgactgcc tgtgcgcccc ccgattcagg gtcggcgagg caatatcgcg
      841 gtgtgcagtc gatcgtcagt gccagcgcac gcttcaagcc ttccagcttt accacggcgg
      901 catcggcggc gccggggcgc ttgacggttt ggccgccgac cgtgctgtcg aattggtccc
      961 acacccaggc gcgcgagcac agatccgggc acccgaccag tttttcgagt gctcgggtca
     1021 ggccgtagcg gtcctcgatc cgggccgggt tcaatttctc ggatttcggc gtatcgacgg
     1081 tcgggcgagt ataaagcggc gcctgatcgt tcaacggcgc cagcgggata tcggcctcga
     1141 cgatgccgtg atggcggacg acgatccggc cagtgtcggt aatccggccg atgacggcga
     1201 aatcgaggtc ccacttggcg aagatgtcgc gggcaagctg ctcgcggtcc ggccgcagga
     1261 ccatcagcat gcgctcctgg ctctcggaca gcatgaattc gtaggctgtc atgccggttt
     1321 cgcgcgccgg cacctggtcc aggtcgagct cgatgccgac attccccttg ccggccattt
     1381 ccaccgacga gctggtcagg cctgccgcgc ccatgtcctg gatggccacg atcgcatcgg
     1441 tggccatcag ttccaggcag gcttcgatca gcagcttttc ggtgaacggg tcgccgacct
     1501 gtacggtggg acgtttttcc tccgtgtcgg cggcgaactc agcggaggcc atggtcgcgc
     1561 catggatgcc gtcgcggccg gtcttggagc cgacatagac caccgggttg ccgatgccgg
     1621 cggcggcgga caggaaaatc cggtccttgc gggcgatgcc cacggtcatg gcgttgacga
     1681 gtgggttgcc gtcgtaggcg tgatgaaaat tgatctcgcc gccgacggtc gggacgccga
     1741 c
//
LOCUS       DASWQN010000281         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1348391, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000281 DASWQN010000000
VERSION     DASWQN010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348391"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1569
                     /locus_tag="VGC82_04665"
     CDS             214..1569
                     /locus_tag="VGC82_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422597.1"
                     /translation="MARGTTEMRSDTKPAADEMKSAYDAAMAFPVEQVNSLRATPEQL
                     MGKASEEAAFGRITIDDGGLTAGTRLHVIEPARAILAQWRQARRRFDVAIEPKMRSIR
                     AVQEAEKEIAGARERAGRDERDAEARLEADEHYIRVRDAFRVAETRYQRMKADHENRD
                     ANMVAFNPAYWVALLCIGLAEWLINYDVFFLFAGVAAIAAGATVVMGVLLAFAAHGHG
                     LLFKQWSYRFGAHRDSIDRRGDWRMLALSTFSLLIVLGAATGSRYAAVMHQAADQPAI
                     NLLGPDAQISFDPMRDVLLSLLWNLMAWAVGVFIAYMAHDKDPDFMDATRQFNGALRR
                     YRRLRRPVVNRLRQLNARLSKDIERYESAARTKAADVAAERALLEQVDRHEDAILAAL
                     TAVVRGNAQLYHASLAQLAASQRGSVTIERVGPRAGQISVNDFRNETVTVTPDMIRGL
                     A"
     gene            1641..2399
                     /locus_tag="VGC82_04670"
     CDS             1641..2399
                     /locus_tag="VGC82_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422598.1"
                     /translation="MVFLAVLIAANLMCKPSWALFDNYGLDAAYCQQPTVRSTVVYID
                     DMMMAEGQTEWATKLAVKLRATLTPGERVSVVRLSPASGQSKEYWSGCWPDYPAAKKA
                     ALANETYILQQNPVDKIADQKKYFVRDFGAALTRIYLDSKRPPDAVRIAADSPPQKQL
                     LRALASDEGRFANSATTIRRSSIPTWLKTAIWGRCSSLPRWILRLMGRSWVPTFATAS
                     STTTAWARMSAGSPPIWNERERSGAARYDRWPQR"
     gene            2405..>2593
                     /locus_tag="VGC82_04675"
     CDS             2405..>2593
                     /locus_tag="VGC82_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422599.1"
                     /translation="MGGDLNVPNTLPLRAYSWPVTLQFDGQALDGRLSLLVGEDGNLV
                     DSSLGISRLGSAAIGGTFK"
BASE COUNT          451 a          790 c          904 g          448 t
ORIGIN      
        1 tgcccggccc cgatcgatcg ccttccgtcc cggcacaaca atcgaatccg ctgaaaacag
       61 ctgccccggg gcagcctttg gcgccgcttc cctccgccgg agtggcacgg ccggccccgg
      121 ctccccactt tcagcccagg acgctttcgc ccgatgtcca gcctaacccg cttggctcgg
      181 ccgtgccgtt ctgaacacga caaggggacc aggatggctc gcggcacgac cgagatgcgg
      241 agcgacacga agccggccgc ggatgagatg aagtcggcgt acgacgcggc gatggcgttt
      301 ccggtggagc aggtgaacag cctacgggcc acgcctgaac aattgatggg caaggcgagc
      361 gaggaagccg cgttcggccg gatcaccatc gacgatggtg ggctgaccgc gggaacccgc
      421 ctgcatgtga tcgagccggc gcgagcgatc ctcgcgcaat ggcggcaggc ccgccggcgg
      481 ttcgacgtgg cgatcgagcc aaagatgcgc agcattcgcg ccgtgcagga ggcagagaag
      541 gaaatcgcag gcgctcggga gcgggctgga cgcgacgagc gggacgccga ggcaaggctg
      601 gaggccgacg agcactacat cagggtgcgc gatgcattcc gggtggcgga gacccgctac
      661 cagcggatga aagcggacca cgagaatcgc gatgccaaca tggtggcctt caacccggcc
      721 tactgggtgg cattgctctg catcggactg gccgagtggc tgatcaacta cgacgtgttc
      781 ttcctgttcg ccggggtggc cgcgatcgcc gccggcgcca ccgttgtcat gggtgtgctg
      841 ctggcattcg cggcgcatgg gcatggcttg ctgttcaagc agtggtccta ccggttcggc
      901 gcgcaccgcg actcgattga tcggcgcggc gactggcgca tgctggcgct gtccaccttt
      961 tccctgctga tcgtcctggg ggccgcgacg gggtcgcgct atgcggcggt catgcatcag
     1021 gcggctgacc agccggcgat caatttgctg gggccggatg cgcagatctc gttcgatccg
     1081 atgcgggatg tgctgctgag cttgctgtgg aacctcatgg cctgggcggt gggcgttttt
     1141 atcgcctaca tggcgcacga caaggaccca gacttcatgg acgcgacacg gcagttcaac
     1201 ggtgcgctcc gccggtaccg ccggctgcgg cgtccggtgg tcaatcggct gaggcagttg
     1261 aacgccaggc tttccaagga catcgagcga tacgaatcgg ctgcccgcac caaggcagcc
     1321 gatgtcgccg cggaacgcgc gctgctggag caggtggaca ggcatgagga cgccatactg
     1381 gcggccttga cggcggtggt gcgcggaaat gcacagcttt accatgccag cctcgcccag
     1441 cttgccgcgt cccagcgtgg ttcggtgacg atcgagcgag tcgggccacg cgccggtcag
     1501 atcagcgtca acgacttccg gaatgaaacc gtcaccgtga cgccggacat gataaggggg
     1561 cttgcgtgat gcgggccggc ggaggttcga agctgtatca gcctttcgga gcagcgcggc
     1621 caggacgtgg gcggcggggc ttggtgttcc tggccgtgct gatcgcagcc aacttgatgt
     1681 gcaagccgtc atgggcgctg ttcgacaact acggcctgga cgcggcctac tgccaacagc
     1741 caaccgttcg cagcaccgtc gtctatatcg acgacatgat gatggcggag ggccagaccg
     1801 agtgggcgac aaagctggcc gtgaagttgc gggcgacgct tactcccggg gagcgggtgt
     1861 cggtggtgcg cctgtccccg gccagcgggc agagcaagga gtactggtcg ggctgctggc
     1921 cagattatcc ggcggcgaaa aaagccgcgc tggcgaacga gacctatatc ctgcagcaga
     1981 accccgtgga caagatcgct gaccagaaga agtatttcgt gcgcgatttc ggtgccgcgc
     2041 tgacgcgaat ctacctcgac agcaagcgac cgccggatgc tgttcgcatc gcggccgaca
     2101 gtccaccgca gaagcagctt ctgcgggcct tggcctccga tgagggacgc ttcgccaaca
     2161 gcgccacgac gatccggcga tcgtctattc cgacatggct gaaaacagcg atttggggtc
     2221 ggtgttccag cctgcctcgc tggatcctcc ggcttatggg cagaagctgg gttcctacct
     2281 tcgccacggc gtcttctacg actacggcat gggcgaggat gtccgcgggc agcccgccta
     2341 tttggaacga gcgcgagcgt tctggagcgg cgcgctacga tcgatggccg caacggtgat
     2401 tggattgggg ggcgatctga acgtccccaa cacgctgccg ttgcgggcgt attcctggcc
     2461 ggtgacgctg cagttcgatg gccaggcgct ggatggccgg ctttcgctgc tggttgggga
     2521 agacggcaac ctggtggaca gttcgctcgg catctcgcgc ctgggttcgg cggcgatcgg
     2581 tggaacgttc aag
//
LOCUS       DASWQN010000282         4878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_29463, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000282 DASWQN010000000
VERSION     DASWQN010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29463"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VGC82_04680"
     CDS             complement(<1..390)
                     /locus_tag="VGC82_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HEY0422600.1"
                     /translation="MNPNQLADEAARLADRALQLANHARGLTMDVAGTVAPEVNPFIF
                     LLAVFLMACFVGYYVVWNVTPALHSPLMAVTNAISSVIIVGAMLATGLATSGWAMLFG
                     FIAVTLASVNIFGGFMVTQRMLSMFRKK"
     gene            complement(412..1551)
                     /locus_tag="VGC82_04685"
     CDS             complement(412..1551)
                     /locus_tag="VGC82_04685"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY0422601.1"
                     /translation="MRLAVLKERRAAETRVAATPDTVKKLVALGLTVAIEAGAGTTAA
                     ISDAEFSAAGAEIAPDAASALSGAGIVFAVQTPGPEERALIPRGALLVCIAGAFADPE
                     LVPSLASAGIDVAAMELLPRITRAQSMDVLSSQSNLAGYRAVVESAATFQRGFPMMMT
                     AAGTVPPARVFVIGAGVAGLQAIATARRLGAIVSATDVRPAAKEEIKSLGASFVGVED
                     AETAGQTGAYAREMSEAFRQKQAELMATTVAKNDIVICTALVMGRRAPVIVTQPMVDS
                     MRAGSVIVDLAAEAGGNCAATVSGQSTTTPNGVTILGFNNWPGRIPVASSSLYARNLL
                     TFLTTFWDKDNQAPKLPETDDIVKGVMLTRGGATVHPNFLPQQAA"
     gene            1844..2620
                     /locus_tag="VGC82_04690"
     CDS             1844..2620
                     /locus_tag="VGC82_04690"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0422602.1"
                     /translation="MALLDGKVGIVTGAASGIGAATARVLAREGAKLVLTDLDDAGGS
                     ALAEEVGGVYLHHDVSEEDGWPAVIAAAEKFGRLDIMVANAGIAVMGNVVDMPLKDWR
                     RQMAINVDGVFLSVKHCIPAMRRAGNGGSIVMLSSVAGLRGSAGLAGYSATKGAVRLF
                     AKSMALECAQAGDGIRVNSVHPGIIATPIWTKMPAGQNRPMDPHTIAENTVPLGKAGE
                     ASEIADGILFLASSMSSHVTGSELVIDGGLTAGRLTRLAQ"
     gene            2617..2889
                     /locus_tag="VGC82_04695"
     CDS             2617..2889
                     /locus_tag="VGC82_04695"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEY0422603.1"
                     /translation="MNLFEITAAVARPDHTVTITWSDGVSGVVDFLPIIDRGGWFTPL
                     RDGDYFAATMIVLPEGQGLTWPEEIDYSADGLRRAVFPNGLPNGCG"
     gene            complement(3015..4172)
                     /locus_tag="VGC82_04700"
     CDS             complement(3015..4172)
                     /locus_tag="VGC82_04700"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0422604.1"
                     /translation="MANTQEIASILRRAAESGDVPGVVAAAADVNGLIFEGGFGTRDL
                     ATGDPMTADTVVWIASMTKAVTAACAMQLVEQGKLSLDGDVAAVLPELARVQVLQGFD
                     ADGKPRLRAPKRPMTLRHLLTHTSGFAYDIWNQDIGHYMKATGTPGILACTNAALSLP
                     LVFDPGDRWEYGISIDWAGKAVEAVSGLTLDRYMHDNLLAPLGMTDTAFKIGEAQRKR
                     LARIHARTQDGLVSTDTELPQDPEFHMGGGGLYSTVGDYLKFAQMILHGGTLNGVQVL
                     RPETVAAMSQNAMGNLVCNPMKTTAPGSSHDVDFIAGMKWGLSFLINPQALPTGRSAG
                     SLAWAGLANSYYWIDPVRQVAGVYATQILPFFDPKAVRTFEAFETAVYATV"
     gene            4687..>4878
                     /locus_tag="VGC82_04705"
     CDS             4687..>4878
                     /locus_tag="VGC82_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422605.1"
                     /translation="MVIAIGLVRLLLVLGHGGATQPDYSTISGRGSFALFPFKVSLRL
                     VSFTMRIGGGQDISDVVPAF"
BASE COUNT          860 a         1477 c         1651 g          890 t
ORIGIN      
        1 ctttttcctg aacatcgaaa gcattcgctg cgtcaccatg aagccgccga agatattgac
       61 cgaggcgagg gtgacggcga tgaacccgaa cagcatcgcc catcccgacg tggccagtcc
      121 ggtcgccagc atcgcgccca cgatgatcac cgaggagatc gcgttggtca cggccatcag
      181 cggcgagtgc agcgccgggg tcacgttcca gacgacgtaa tagcccacga agcaggccat
      241 caggaaaacc gccagcagga agatgaatgg attgacctcg ggtgcgacgg tgccggcgac
      301 gtccatggtc aggccgcggg cgtgattggc gagttgcagc gcgcggtcgg cgaggcgtgc
      361 ggcttcgtcc gccagttgat tggggttcat ctgcgatcgg tctccggtaa ctcaggcggc
      421 ctgttgcggc aggaaattcg ggtggaccgt ggccccgccg cgggtcagca tgacaccttt
      481 cacgatgtcg tccgtttccg gcagctttgg cgcctggttg tccttgtccc agaacgtggt
      541 caggaacgtc agcaggttgc gcgcgtacag cgacgacgag gccacgggga tgcggccggg
      601 ccagttgttg aagccaagga tggtgacgcc gttgggcgtg gtggtgcttt ggccggaaac
      661 ggtcgccgca cagttgccgc cggcctctgc cgcgaggtcc acgatcacgc tgccggcccg
      721 catgctgtcc accatcggct gcgtcacgat gacaggcgcg cggcggccca tcaccagggc
      781 ggtgcagatg acgatgtcgt ttttcgccac ggtggtcgcc atcagttcgg cttgcttctg
      841 acggaacgcc tcgctcattt cgcgggcata ggcgccggtc tggccggcgg tttccgcgtc
      901 ctcgacgccg acgaacgatg cgccgagcga tttgatctct tccttggcgg cgggcctgac
      961 atcggtggcg gacacgatgg cgcccagccg gcgggcggtg gcgatggcct gcaaaccggc
     1021 gactcccgcg ccgatgacga acacccgggc tggcggaacg gtgccggcgg cggtcatcat
     1081 catgggaaag ccgcgctgga acgtggcggc agattccacg acggcgcgat agccggcgag
     1141 attggactgg ctggacagca cgtccatgga ctgtgcccgc gtaatccgcg gcagcagctc
     1201 catcgccgcc acgtcgatgc cggcggaagc gaggctgggg acgagctccg ggtcggcgaa
     1261 ggctccggcg atacagacca gcagcgcccc gcgcgggatc agggcgcgtt cctcgggacc
     1321 aggggtctgc accgcgaaga caatgcctgc gcccgacaaa gccgaggccg cgtcgggggc
     1381 gatttcagcc ccggcggcgg agaactcggc gtctgaaatc gcggctgttg ttcccgcccc
     1441 ggcctcgatc gcgaccgtca atcccagggc gaccagcttt ttcaccgtgt ccggagtcgc
     1501 ggccacgcga gtttcggctg ctcgccgttc ctttagcacc gccaagcgca tgcatctgat
     1561 cctttacgca cggggagcca ttcgggcacg acatgcacct gttcccccca ggctatgggc
     1621 aagcctgaat gcacggtgga cgccgcatgt gcaagagggt gacggtcgcg atttagtgtc
     1681 ctggtcacct cgcggcggga cagaactggt taagcggtag ccgccgacca gccgaaccgg
     1741 cagaagccgc cgtgccatcg ataccgattg cacgcttgga cagacgctct tggaacccgc
     1801 gccgaggaat gggaagatcg gtggacaatc aatggaggaa accatggcac tactcgatgg
     1861 caaggtcggc atcgttacag gtgcggcgtc cggcatcggc gcggcaaccg ccagggtatt
     1921 ggccagggaa ggcgcgaagc tggtcctgac cgatctggac gacgccggtg ggtcggcgct
     1981 agccgaggaa gttggaggcg tttatctcca ccacgacgtt tccgaggaag acggctggcc
     2041 ggcggtcatc gccgcggcag agaagttcgg gcggctggac atcatggtgg cgaacgccgg
     2101 catcgccgtc atgggcaacg ttgtcgatat gccgctgaag gactggcgcc ggcagatggc
     2161 gatcaacgtc gatggcgtgt tcctttccgt gaaacactgc attccggcga tgcggcgggc
     2221 cgggaatggc ggctcgatcg tgatgctgtc ttccgtggcg gggctgcgcg gttcggcggg
     2281 cctcgccggc tacagcgcga ccaagggtgc tgtgcggctg ttcgccaagt cgatggcgct
     2341 ggagtgcgcg caggccggcg acgggatccg agtgaactcg gtgcatccag ggatcatcgc
     2401 gacaccgatc tggaccaaga tgccggctgg ccagaaccgg cccatggacc cccacacaat
     2461 cgcggaaaac accgtgccgc tgggtaaagc cggcgaggcg tcggagattg ccgacggtat
     2521 cctgttcctg gcgtcgagca tgtcgagcca cgtcaccgga tcggagctgg tgatcgacgg
     2581 cggcctgacc gcgggacgcc tcacccggct ggcgcagtga acctgttcga gatcactgcg
     2641 gctgtcgccc gtcccgatca caccgtgacc atcacctggt ccgacggtgt atcgggcgtc
     2701 gtggatttcc tgccgatcat cgaccggggc ggctggttca cgccgctccg ggacggcgat
     2761 tacttcgccg cgaccatgat cgtgctgccg gaaggccagg gactgacgtg gccggaggaa
     2821 atcgattact cggcggacgg cttgcggcgg gcggtttttc cgaacgggct gccgaacggg
     2881 tgtggttaga gcgtgatcac gctgggcgga acgtccgtcg tccaaacgac gcgcgattcg
     2941 cacgatgcgt gcgaatcaca caataaacaa gaaggcagac gacttgcggc ccgcacagtc
     3001 aggctgaaaa tcgtctagac tgtggcgtaa accgcggttt cgaaggcctc gaatgtccgc
     3061 accgccttgg gatcgaaaaa cggcagtatt tgcgtggcat aaacgccagc gacctgccgc
     3121 accggatcga tccagtagta ggaattggcg agacccgccc acgccaggct tccggcggaa
     3181 cgtccggtcg gaagcgcctg tgggttgatc aggaaactca ggccccactt catgccggcg
     3241 atgaaatcca cgtcgtgcga gctgcctggc gccgtggttt tcatcggatt gcaaaccagg
     3301 ttgcccatgg cgttctgcga catggcggcc acggtctccg gccgcaatac ctgaacgccg
     3361 ttgagcgtgc cgccgtgcag gatcatctgg gcaaatttca ggtagtcgcc gacggtcgag
     3421 taaagtccgc cgccgcccat gtggaattcc ggatcctgtg gaagttcggt atccgtcgaa
     3481 accagaccgt cttgcgtgcg ggcgtggatc ctggcgaggc gcttgcgctg cgcctcgccg
     3541 atcttgaacg ccgtatcggt cattccgagc ggggccagca gattgtcgtg catgtatcgg
     3601 tccagggtca ggcccgagac cgcctcgacc gctttgccag cccagtcgat cgagatgccg
     3661 tactcccacc tgtcgccagg gtcgaacacc agcggcaagg atagcgccgc gttggtgcaa
     3721 gcaaggatgc cgggcgtgcc ggtcgccttc atgtaatggc cgatgtcctg gttccagatg
     3781 tcgtaggcaa agccggaggt atgggtcagc agatggcgca acgtcatcgg tcgcttcggc
     3841 gcccgcagcc ggggtttgcc gtcagcgtcg aagccctgga gcacctgcac gcgagccagt
     3901 tcgggcagca cagcggcgac gtcgccgtcc agcgagagct tgccttgctc gaccaactgc
     3961 atcgcgcagg ccgcggtgac cgccttcgtc atcgacgcga tccaaaccac ggtgtcggcc
     4021 gtcatgggat cgccggtcgc caggtcgcgc gtgccgaagc cgccctcgaa gatcaagcca
     4081 ttgacgtctg ccgccgcggc cacgacgccg ggcacatcgc cggattcggc cgctcggcgg
     4141 agaatggagg cgatttcctg cgtattggcc atgactttcc ccttttgtgt gctgttgatg
     4201 gacagtctct atttgttttg tgtcggcaga taggtgaact ggctccaaag ttattgccgc
     4261 cggggtcagc gtccgtctcg catccgccgc caaactgggc agatcatgct agacggcatc
     4321 gcaaattaca tccgggttga tctgactata ggcgtgccgt agccggttgc ccatggcacg
     4381 gatgtcgccc taacggctgg ccgggcattg gctcgccgaa gcggaacatg gcctcccata
     4441 cttgcttcgg gcagcgcggt tgaaacacgg tgtggcgtca acataggttg gcgaggcggg
     4501 ttcagcttcg acattagcgt cgacgctggc tgcgattttc aacacggagc gcacggaggc
     4561 gcgcggaggg cacggagaaa atttgcgtcg tatgcgatac ggagcgggtt ctgggtggac
     4621 gcagagcatt tctccgcagt cacccgatac atatgcgagc agaaggataa ttgaactctc
     4681 gacgagttgg tcatcgcgat cggccttgtg cggctgctcc tggtgctggg gcatggcggg
     4741 gcgacacagc ccgattattc cacaatttcg ggccgtgggt cgttcgcgtt attccctttc
     4801 aaggtgtcct tgcggctcgt cagctttacg atgcggatcg gtggcgggca ggatatttcg
     4861 gatgtagtcc cggctttt
//
LOCUS       DASWQN010000283         3426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_952259, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000283 DASWQN010000000
VERSION     DASWQN010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952259"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2538)
                     /locus_tag="VGC82_04710"
     CDS             complement(<1..2538)
                     /locus_tag="VGC82_04710"
                     /inference="COORDINATES: protein motif:HMM:NF033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HEY0422606.1"
                     /translation="MGRWDQHYLGRESFPEALSALEIQRFFTPTPEEKQVVLQRRSTT
                     NRIAFALQIGFLKMTGRLLNSVELVPRDVLSHLGGVVECEPPRIASIRAFYRRRRTLF
                     EHQAAARALLRRSEVSEHGLRGLTAFLRREAVGIYNSVDLAAKARVWLIDHGYLLPTE
                     REIRRQTIRALRHQEQVVLEAVRAATDEALRGAWPRRLLEPAPDNERTRLEWLWTPPT
                     SKQASELDDHLAKVKFLQELGADRLKIEDLPLAGLEHFHRRVTSRKPAALLTIREPRR
                     TLELACFLRLQLMRLTDTALDLVDRRIATQWREAREQAEEGQGGRLQRFRTLLGNLTA
                     LAEDDALGIEALRERLRALVAPFKPELDNTPVLATRRALSERTLVLNRVLTSARAVGL
                     DLPADHKLAMAFAALDGLSGSRALPAGTDNPFGRSWQPLIDQPDRSAALKSYTAATAM
                     LLKRALNNRSVTAKDSLLHKSTEARLIPPAMWRRDRHRFFRGLSIPPNPEHYLRRVEE
                     ALDAGMARLAAAVEGGLVSIDDDGVHLPRRKRAPENPAVARAHKSLAGHYGTPQLSDV
                     LIEVDNDARFSWALLGRSAHSAAELITLYVALLALGSDLTVAALDRMVAGVEADALAA
                     MMQRLGGGARFRVANDAVVGFMRRHAVTRLWGRGLSASADMMSLDATRHLWNARLDPR
                     RKGPAIGTYPHVLDQWPIFYDQPIILGKRQAGAAIEGALRQTAVEKLERVAVDTHGFT
                     HFAMAVGKFCGFDLCPRLAGIRSRKLYLPRGYHGTVPDVLKPIVSQETISRKAIVRGW
                     DGFARLSASIKDGWYPAPDALEQYGSVSQGDPIRSTGTALGKLLRSVY"
     gene            complement(2539..3108)
                     /locus_tag="VGC82_04715"
     CDS             complement(2539..3108)
                     /locus_tag="VGC82_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY0422607.1"
                     /translation="MALVGYVRVSTAAQDGALQRDALGKAGCSKLFEDKASGAKSDRP
                     GLNDALTYIRAGDVLVVWKLDRLGRSLSHLIDTVTELGGRGVGFKSLTEGVDTSTPNG
                     RLVFHIFGSLAEFERDLIRERTGAGLAAAVARGRRGGRKPVVTPEKLARAKALIAEGL
                     NVREAAGRVKVGKTALYEALVDKPVQASP"
BASE COUNT          666 a         1122 c         1028 g          610 t
ORIGIN      
        1 gtagacggac cgcagcagct tgccgagggc ggtcccggtc gagcgaatcg ggtcgccttg
       61 agagacggaa ccgtattgct ccagagcgtc gggagccgga taccaaccat ccttgatcga
      121 ggcgctgagc cgtgcgaagc cgtcccagcc acggacgatc gcttttcggg aaatcgtttc
      181 ctgagagacg atcggtttca gaacatcggg aaccgtgccg tgatatccgc gcggaagata
      241 gagtttgcgg cttcggatgc cggcaagtct gggacagaga tcgaagccgc agaacttgcc
      301 gaccgccatg gcaaaatgcg taaagccgtg cgtatccaca gccacgcgtt ccagcttttc
      361 gacggcggtc tggcgcaagg ctccctcgat cgcggcgccg gcctgacgtt ttcccaatat
      421 gatcggttgg tcatagaaga tcggccattg atccagcaca tgaggatagg tgccaatcgc
      481 cggccctttg cggcgcggat caagccgggc gttccagaga tggcgggtgg cgtccaggct
      541 catcatatcg gccgaggcgg aaagtccgcg tccccagagg cgagtgaccg catgccgccg
      601 catgaagccc acgacagcgt cgttggcgac acgaaaccgc gccccgcctc caagtctctg
      661 catcatcgcg gcaagcgcat ccgcctcgac gccggcgacc atgcggtcca aggccgcaac
      721 ggtcagatcc gaacccaagg ccaacagtgc aacgtagagg gtgatgagtt ccgccgccga
      781 atgcgcagac cgtcccaaca gcgcccacga gaagcgggca tcgttgtcga cctcgatgag
      841 aacgtccgat aactgcggtg tgccatagtg tcccgctagc gatttgtgcg cgcgtgcgac
      901 cgccgggttc tccggcgcac gcttgcggcg cggcagatgc acgccatcat cgtcgatcga
      961 caccaggccg ccctcgaccg cggcggccag tcttgccatg ccggcgtcca aggcttcttc
     1021 cacgcgccgg aggtaatgtt cggggtttgg tggaatcgaa aggccgcgaa aaaagcgatg
     1081 acggtcccgc cgccacatcg ccggcggaat gagccgtgcc tccgtgctct tgtgaagcag
     1141 gctgtccttc gcggtaacag aacgattgtt caaggcgcgc ttgagcagca tcgcggttgc
     1201 cgccgtgtag ctcttcagcg ccgcggatcg gtccggctga tcgatcagag gctgccacga
     1261 acgaccgaac ggattgtccg tcccggcggg caatgccctg gatcccgaca gaccatccaa
     1321 cgccgcgaat gccatcgcga gcttatggtc tgccggcaga tcgagaccaa cggcacgggc
     1381 ggatgtcagc acacggttca gtacgagtgt ccgttccgac aacgcccggc gggtcgccaa
     1441 gaccggcgta ttgtccagtt ccggtttgaa tggagcgacc agagcgcgca atctctcccg
     1501 caaggcttcg atgccgaggg catcatcctc cgccaaagcg gtcagattgc ccaagagcgt
     1561 ccgaaaccgt tgcagtcgtc caccttggcc ttcttcggcc tgttcgcgcg cctcgcgcca
     1621 ctgggtggcg atgcggcgat cgaccagatc caatgcagtg tcggtcaacc gcatcagttg
     1681 caggcgtaga aagcaggcca attcgagcgt ccggcgcggc tctctgatcg tcagaagcgc
     1741 ggccggcttg cgggacgtga cccggcgatg gaaatgctcc agtcccgcca atggcaggtc
     1801 ttcgatcttc aggcgatcgg cgcccagctc ctgcagaaac ttcactttgg ccaggtggtc
     1861 atccaactcg gaggcctgtt tgcttgtcgg cggcgtccac agccattcca gccgcgtccg
     1921 ctcattatcc ggggccggtt ccaggagccg cctcggccag gccccgcgca acgcttcgtc
     1981 cgtcgcggca cggacggcct ccaacacaac ctgttcctga tggcgcaagg cccggatcgt
     2041 ctgacggcgg atctctcgtt cggtcgggag tagatagccg tggtcgatca gccaaacccg
     2101 ggctttggcg gcaagatcca cactgttgta gataccgacg gcttcgcggc gcaaaaacgc
     2161 ggtcagtccc cgcaaaccgt gctcggacac ctcgctccgc cgcaatagcg cgcgggctgc
     2221 ggcctgatgt tcgaacaaag tccgccgccg ccggtaaaac gcacggatcg aggcaatgcg
     2281 cggcggctcg cattcgacaa ccccgcccag atgcgaaaga acatcccgcg gcacgagttc
     2341 cacggaattg agcaatcgcc ccgtcatctt caggaagccg atctgcaggg cgaaggcaat
     2401 gcggttggtc gtcgagcggc gttgcagaac gacctgcttc tcttccgggg tcggcgtgaa
     2461 aaaacgctga atctccaacg ccgacagcgc ctccggaaag ctctcccgcc caagataatg
     2521 ctgatcccaa cggcccaatt acggggaagc ctgtaccggc ttgtcgacaa gcgcttcata
     2581 caaagccgtc ttgccgacct taacccgccc cgccgcctcc cgcacgttca gcccctccgc
     2641 gatcagcgcc ttcgccctgg cgagtttctc cggcgtgacc acgggtttac ggcctccccg
     2701 gcgtccgcgg gccaccgccg ccgccaagcc ggcgccggtg cgttcacgga tcagatcgcg
     2761 ttcgaattca gccagcgacc cgaagatatg aaacacgagc cggccattgg gggtgctggt
     2821 atccacgccc tcggtcagcg acttgaagcc gacgcctcgc ccgcctagtt cggtcactgt
     2881 atcgataagg tgtgataacg accgtcccag gcgatccagc ttccagacca ccaatacgtc
     2941 ccccgcgcgg atataggtca gcgcatcgtt cagtcccggt cgatccgact tggcgccgga
     3001 ggccttgtcc tcgaacagct tgctgcatcc cgccttgccc agcgcgtcgc gctgtagcgc
     3061 cccatcctga gcggctgtgg agacacgcac atacccgacc agcgccatgc gacccgtcca
     3121 cgtccgcctt tccgtccggc attcaacagg tccggattgc cattgtcaat aaggttcacg
     3181 gacaagaatt cgtccgtccg ggaaacgttc gtttgacgga cagaaccctg gcgaacccgg
     3241 aaaaagctga atctgccaat atttcaattg gttggcactg ggcatcgcaa tatttatcaa
     3301 tgatttcagt aatttgctta acggcacttt ctgcacgtat atgacgaaaa ggccagatcg
     3361 gcgatgcgcg gccctggcgc acccagcggg tgctcagtca tgtgcagtgg gaccaggatg
     3421 cggcgc
//
LOCUS       DASWQN010000284         3413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1876709, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000284 DASWQN010000000
VERSION     DASWQN010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876709"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VGC82_04720"
     CDS             complement(<1..836)
                     /locus_tag="VGC82_04720"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0422608.1"
                     /translation="MPYRLKKIADQVMVITGASSGIGLATAYAAARQGARVVLTARNE
                     SALRDAVERIRAQGGRATYVVADVANKEDLEKVAQTAVSEFGGFDTWVNNAGLGIFGR
                     LEEVSDEDHRRLFDVNFWGLVYGTKIAAAHLKTKGGAIINLGSLVSDVAFPIQGMYAA
                     SKHAIKGFTDSFRMEMEEAGAPISITLIKPASINTPFQSHAKNYLSQEPKLPPPVYAP
                     ENVADAIVHAAAHGGRDYYIGGGGKVMSTLNKHVPALVDWVGARTVHKQSTFDRPASQ
                     AR"
     gene            complement(947..2383)
                     /locus_tag="VGC82_04725"
     CDS             complement(947..2383)
                     /locus_tag="VGC82_04725"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0422609.1"
                     /translation="MDPTFLSATQLAELVRSREIGALELLDHYIARTEQLDGRINAVV
                     VRDFDRARARAKALDAQPDKTAPLFGVPTTVKESFDVAGLPTTLGHVELRNGNAELSS
                     LSVRRLESAGAVIFGKTNVPVDLADWQSYNPVYGTTSNPWNKDHTPGGSSGGSAAALA
                     AGLTGFEMGSDIGGSIRVPAHFCGVYGHKPTFGLLPNFGNPATSAAAGVDIAVIGPIT
                     RSAADLAVALDILARPDPDETALTLTLPAPRFSRLNELRVAVWAEQPGQPTDSETVAA
                     ILALADGLERQGATVSRTARPGFDPTEAYHLFLRLLDTGWSGRSTDEQVQAKRERAAT
                     VSDADMGADAIMLRTVDMHHRTWLGLNEQRFRLRRTWSAFFREWDVLLCPAFSTPALP
                     HRQDGMPWDRRLTVEAREISYGDLLFWPGLIGGYHLPATVAPLGQTKSGLPLGVQIVG
                     PIHGDRTTIAAASLLEQAWAGFRPPPGW"
     gene            complement(2493..3098)
                     /locus_tag="VGC82_04730"
     CDS             complement(2493..3098)
                     /locus_tag="VGC82_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0422610.1"
                     /translation="MPRLITGSETGSRILDVAERLVQTRGFNAFSYADIAAELHVTKA
                     SLHYHFATKADLGRSLIERYRTSFVAALDRIGRGEGDAGPKLHAYVDIYADVLDNGRM
                     CLCGMLAAEYATLPASMQDGVRRFFDANEAWLVGMLVDGRDKHQLTFTGEAVDVARVL
                     IAALEGAMLLVRSGLDTSHLRSVARRLLADLGIDARAEVAG"
BASE COUNT          607 a         1077 c         1108 g          621 t
ORIGIN      
        1 tcccgtgcct ggcttgccgg gcggtcgaac gtggactgct tgtgcacggt gcgcgcgccg
       61 acccagtcca ccagggccgg cacatgcttg ttcagcgtgc tcatgacctt gccgccgccg
      121 ccgatgtagt agtcgcgtcc gccatgagcg gcggcatgaa cgatcgcgtc cgccacattt
      181 tccggcgcat agacgggtgg tggcagcttc ggttcctgcg ataggtagtt ttttgcgtga
      241 ctctgaaatg gtgtgttgat cgacgcgggc ttgatcagcg tgatggatat cggggcgcca
      301 gcctcctcca tctccatgcg gaacgagtcg gtgaaccctt tgatcgcatg cttggaggcg
      361 gcgtacatgc cctgaatagg aaacgcgacg tccgaaacga ggctgcccag gttgataatc
      421 gcgccgccct tcgtcttcaa atgggctgcg gcgatcttgg tgccgtaaac caggccccag
      481 aaattcacgt cgaacagccg ccgatggtct tcgtcggaga cttcttccag ccgtccgaag
      541 ataccgaggc cggcgttgtt cacccacgtg tcgaaaccgc cgaactcgct gacggcggtc
      601 tgcgcgactt tctccagatc ctccttgttg gccacgtccg cgacgacata ggtcgctcgg
      661 ccgccctggg cgcggatgcg ctccacggca tcccgaagcg cggactcgtt tcgtgcggtc
      721 aacacgaccc gggcgccctg ccgagcggct gcgtaggcgg tggcaaggcc gatgccgctc
      781 gatgccccgg tgatgaccat gacctggtcg gcgattttct tcaggcgata cggcatgatt
      841 tctccagggg tgctgatccg gcgccttaac tgccgcctgg agcggccggt tcgcgcacga
      901 ctccgtgacg ctgggtagcg ccgccggtcc cgtggagaag cgggatttac cagcctggcg
      961 gtggccggaa cccggcccag gcctgttcca gcagcgaggc cgcggcgatc gtcgtgcggt
     1021 ctccgtggat cggtccgacg atttgcacgc ccagcggcag gcccgatttg gtctgaccga
     1081 ggggcgccac cgtcgcgggc agatgatagc cgccgatcag tccgggccag aacagcaggt
     1141 cgccgtagct gatttcccgc gcttcgacgg tgaggcgccg gtcccatggc atgccatcct
     1201 gccggtgcgg cagcgccggg gtgctgaagg cggggcacag cagcacgtcc cactcgcgga
     1261 agaacgcgct ccaggttcgc cgaaggcgga aacgttgttc gttcagtccc agccaggttc
     1321 gatgatgcat atccacggtt cggagcatga tggcgtccgc ccccatatcg gcatcggaca
     1381 ccgttgccgc ccgttcccgc ttggcctgaa cttgctcgtc ggtggaccgg ccgctccagc
     1441 cggtgtccaa caggcgaagg aacaaatgat aggcctcggt ggggtcgaag ccggggcggg
     1501 cggtgcggct gacggtggca ccttgccgtt ccagtccgtc cgccagcgcc aggatcgccg
     1561 ccacggtttc ggagtccgtg ggctggccgg gctgctccgc ccacactgcc acccgcagct
     1621 cgttcagccg ggagaaacgc ggcgccggca gggtcagcgt caatgcggtt tcatccggat
     1681 cgggcctggc caggatgtcc agcgcgacag cgagatcggc ggcggagcga gttatcggcc
     1741 cgatcacggc gatatcgacc ccggcggcgg cgctggtcgc cgggttgccg aaattgggca
     1801 gcagcccgaa cgtgggcttg tggccataaa cgccgcagaa atgcgccggc acgcggatgg
     1861 agccgccgat gtcgctgccc atctcgaacc cggtcaaccc agcggcgagc gccgcggccg
     1921 atccgccgga ggatcctcca ggcgtgtggt ctttgttcca cgggttggag gtggtgccgt
     1981 aaaccggatt gtagctttgc cagtccgcca gatcgacggg aacgttggtc ttgccgaaga
     2041 tcaccgcccc ggccgactcc aggcgccgaa ccgagagcga cgacagttcg gcgttgccgt
     2101 tcctcagttc gacgtgcccg agcgtggtcg gcaggccggc gacgtcgaag ctttccttga
     2161 ccgtggtcgg aacgccgaac aacggcgcgg tcttgtcggg ctgagcatcc agggcctttg
     2221 cccgcgcccg cgctcggtcg aagtcgcgaa cgaccacggc gttgatgcgg ccatcaagtt
     2281 gttcggtgcg cgcgatgtaa tggtccagca attccaaagc accgatctcg cggcttcgga
     2341 caagctccgc cagttgggtg gcggaaagaa acgttgggtc cattggtgct gtccctccat
     2401 tgcggcgccg tgactttaga gcgcatctac gccgactgga aaagctgcga gccagcgccc
     2461 acgaaatgct ggccgctggg tgtgatcatg cttcagcccg cgacttcggc tcgcgcgtcg
     2521 attccaagat cggccagcag gcgccgggcg accgagcgta aatgggatgt atcgaggccg
     2581 gaacgcacca gcagcatcgc cccttccagc gcggcgatca gaacgcgggc gacatcgacc
     2641 gcctcaccgg tgaacgtcag ttgatgcttg tcacggccgt ccaccagcat gccgaccagc
     2701 cacgcttcgt tggcatcgaa gaagcggcgg acgccatcct gcatggacgc gggcagcgtg
     2761 gcgtattcgg ccgcgagcat cccgcacagg cacatgcggc cattgtcgag gacatccgcg
     2821 tagatatcga cataggcgtg cagtttcggt ccggcatcgc cttcgccgcg gccgatccga
     2881 tcgagtgcgg ccacgaagga cgtgcggtaa cgctcgatta aacttcgccc caggtcggcc
     2941 ttggtcgcga aatgatagtg caacgaggct ttcgtcacat ggagttcggc ggctatatcc
     3001 gcgtagctga acgcattgaa cccgcgtgtc tgcaccaggc gttccgccac atccaagatg
     3061 cggctgccgg tctcagagcc agtaatcagc ctaggcatcg cagtccttcc gacactgaat
     3121 acaaccatga agagggcgac ctgtaattgc acacgtccgt gacaggctgc ttatctactg
     3181 gaaggcaaat taacttactg ataggttaat agcgctttga gtgccgaacc tccatcggaa
     3241 agcggcatgc gccagcccga tcaggaaaga gcccgatcag gaaaagcgct ctcgtttatc
     3301 ggtgtcacgc gctgctttat cggtgtcacg cgctggcgaa cggcgtgcga ccccgaagga
     3361 gataaacaaa caaccgcgtt tagcaaacag gagaaggttt cgatgcaaga act
//
LOCUS       DASWQN010000285         4886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_556944, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000285 DASWQN010000000
VERSION     DASWQN010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556944"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VGC82_04735"
     CDS             complement(<1..1100)
                     /locus_tag="VGC82_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0422611.1"
                     /translation="MNYEPVDVLVVGAGNAAANAALSAHASGASVAMLETAPPEAKAG
                     NSAFTGGAFRFVYDGIEDLLKLDPSIADLDLATIDFGTYTTEQYFDDMGRLTDYRCDP
                     DLTEVLIGRSYQSALWLKQHGVRFQPALGRQAFKVGGKFKFWGGLACHIHGGGLHLVA
                     ALHAALEKARIPVLYDTTAYQLLTDDARIQGVKVRHGGRSYDLRAKSVVLACGGFESN
                     AEMRARYIGPNWDLAKVRGTRFNNGYGHKMAMDIGAASAGHWSGAHAVQWDMNAPSYG
                     DISIGDRFQKHNYPFGVILNARGERFLDEGLDFHSYTYAKYGHEVMKQPGLFAWQVFD
                     QKIVNLLRDEYRIARITKETAITLEELVSKLTG"
     gene            complement(1103..1984)
                     /locus_tag="VGC82_04740"
     CDS             complement(1103..1984)
                     /locus_tag="VGC82_04740"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY0422612.1"
                     /translation="MIHSHSQWGPADQIGAGNLLTVEKRLAALASVRHGRIYDVSHDI
                     FMGAPYMAPNQTPFLMSIFGSWRDTIKRRRKMGAVNDAGTNLERIEMTTHVGTHIDAL
                     GHFSNGDMLYNGNAAADVVTDWGLDRLGIEHAPPMITRAVLFDVGGATHLSPGQTVTP
                     DDLKRAADAGGFAVESGDIALIRTGWGRYFGTDNARYLEGEPGIDVPAAKWLIDQGIV
                     AIGADNMAVEVLPNPDKSVMMPVHQFALAECGVYLIENLALEELAAARVHAACFILLA
                     TKIRGATGAPVRPIVMV"
     gene            2066..3076
                     /locus_tag="VGC82_04745"
     CDS             2066..3076
                     /locus_tag="VGC82_04745"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY0422613.1"
                     /translation="MTVMSSRRTLLRSAGGLAGILAAGRAPAFAQATPKKMQIAAVNP
                     VPESGAIALDWMAKALTERTKGELDVTYHGGTLLTKEIDIMNAVKGGNIAMGNPGGAS
                     ATLFPEMGVFLVGYLISSYQQAYAIMNGKVGDDLDKLFQQKYGVKVIFYFDYGFRHFW
                     NSKRAINVPKDLRGLKLRSQPSKVFADTVNSLGGVAVPLGWAEVITAAQQGVIDGADL
                     PVVNMVPLKAYEVSKYYSLTGHNYGATLIAMNLQMWNDLRPEQQRLVLDTGREAQGVV
                     RKVTEDVDTLASAKEQLESRGMIVNQPDPAPFIDIAKQKLWPQYEKQYSGLWEQIVST
                     KV"
     gene            3082..3588
                     /locus_tag="VGC82_04750"
     CDS             3082..3588
                     /locus_tag="VGC82_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY0422614.1"
                     /translation="MRLLTLLPRVFLGGLVLFAISVMLYGVFARYVLLPITDWLDIDQ
                     PNFFWVEEVGETTLAWLTMIGAAIAVKERSHFALNLVTHRFSTRAKAVLHVAHNMLIA
                     LFGGLVAWLGFKLVLLNSMLTSPALEMSLGWLYGSTVAGGILIVLYALDLARKPAGPE
                     HSFADVRE"
     gene            3594..4877
                     /locus_tag="VGC82_04755"
     CDS             3594..4877
                     /locus_tag="VGC82_04755"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY0422615.1"
                     /translation="MVLFATLVTFLILLVLSLPIVFCLGAAGVIGLLTAGLPLQQLGS
                     SIVSASQSWVLLAIPSFVFAGSLMERAGMSTALVELARALVGWLRGGLGMSVIVVAYF
                     FSDICGSKMAEVSALGSALMPSLKKAGYRPEDGASLIAAGTAMGMLVPPAIFMIVISA
                     VTNQSAVALFLAGFIPAATIGACLCVLVLIQAHVLNWPKDTRTSLARLLTALKNAAVP
                     LVIPVVILGGFYMGAFTATEAGAIVALYSLLAAKFYYRNITWMQVLVLAYEAGVLTGV
                     VVFLLAVATIFQYLMGLSGMPEFLGWALGPLEKTPWLFLTGVALLTLVFGMVLEGLPA
                     AVVLIPVVFPIATKIGIHPVHFDIVQTAAVGIGLFTPPLGVGLLMALRFADVSVGQHA
                     KAYWPYLLALVIGLMLIICIPELSLFLPRSAGLIR"
BASE COUNT          827 a         1481 c         1576 g         1002 t
ORIGIN      
        1 cccgtcaatt tacttacaag ctcctccagg gtaattgccg tttctttagt aatacgagcg
       61 atgcggtatt cgtcgcgtaa caggttgact attttttggt cgaatacttg ccaggcgaac
      121 agtccgggct gcttcatgac ctcgtggccg tatttggcgt acgtgtagct atggaaatcc
      181 agaccctcat ccaggaaccg ttcgccgcgc gcattcagga tgacgccaaa cgggtagtta
      241 tgtttctgga agcggtcgcc gatggagatg tcgccgtagg acggcgcgtt catgtcccac
      301 tgcaccgcgt gcgcccccga ccagtgcccg gccgaggcgg cgccaatgtc catcgccatc
      361 ttgtggccgt agccattgtt gaagcgggtg ccgcggactt tcgccaggtc ccagttcggg
      421 ccgatgtagc gggcacgcat ctcggcattt gattcgaagc cgccgcaggc cagcacgacg
      481 gatttagccc gcagatcgta tgaccggccg ccatggcgca ccttcacgcc ctggatgcgg
      541 gcgtcatcgg tcagcagttg ataagcggtc gtgtcataca gcactgggat ccgcgccttt
      601 tccagcgccg cgtgcagcgc cgccacgaga tgcaggccgc cgccatggat atggcacgcc
      661 aggccacccc agaacttgaa tttcccaccg accttgaacg cctgccgccc aagcgccggc
      721 tggaaccgta cgccgtgctg cttcagccaa agggcggact gataagacct gccgatcagc
      781 acctcggtta gatctggatc gcaccggtag tcggtcagcc ggcccatgtc gtcgaagtat
      841 tgttcggtcg tgtaggtgcc gaagtcgatg gtcgcgaggt ccagatcggc gatggacggg
      901 tccagcttca gcaggtcttc gatgccgtca tagacgaagc gaaaggcgcc gccggtgaac
      961 gccgagttgc ctgccttggc ttccggtggc gcggtctcca gcatggcgac cgacgcaccg
     1021 gacgcatgcg cggacagcgc cgcattggcg gcagcgttgc cggcgccgac gaccaggaca
     1081 tcgaccggtt catagttcat gtctatacca tcacgatggg acgtaccggc gcgcccgtgg
     1141 cgccgcggat cttggtggcc agcaggatga agcacgcggc atgaacacgc gctgccgcca
     1201 gttcctccag cgccaggttc tcgatcagat agaccccgca ttcggccagg gcgaactgat
     1261 gcaccggcat catcacgctt ttgtccgggt tgggcagaac ctcgacagcc atgttgtcgg
     1321 cgccgatggc gacgatgccc tggtcgatca gccactttgc cgccggcacg tcgatgccgg
     1381 gctcgccttc caggtagcgg gcattgtcgg tgccgaaata gcggccccag ccggtgcgaa
     1441 tcaacgcgat gtcaccggac tccaccgcga agccaccggc gtcggctgcc cgttttaggt
     1501 catcgggcgt gaccgtctgg ccgggcgaga gatgcgtggc accgccgaca tcaaacagca
     1561 cggcgcgggt gatcatcggc ggcgcgtgtt cgatccccag gcggtccagc ccccaatcgg
     1621 tcaccacatc cgcggcggcg ttgccgttgt agagcatgtc gccattggaa aaatgtccga
     1681 gcgcgtcgat gtgggtgccg acatgggtgg tcatctcgat gcgctcgagg ttggtgccgg
     1741 cgtcgttgac cgcccccatc ttgcgacggc gcttgatggt gtcccgccag gacccgaaga
     1801 tcgacatcag gaacggggtc tggttcggtg ccatgtatgg cgcgcccatg aagatgtcgt
     1861 ggctgacgtc gtagatacgg ccgtgacgaa ccgatgccag cgctgccagg cgcttttcga
     1921 ccgtcagcaa gttgccggcg ccgatctggt cggccggacc ccattgtgaa tggctgtgta
     1981 tcatctcgat gctccacccc gttggccaca ccgtgccatt gctgtaccat attcggcaag
     2041 caaccaagat cgccgaggga ataccatgac cgtcatgtcg tctcgccgca cgctgctacg
     2101 ctctgccggc ggcctcgccg gcatcctggc cgcgggccgg gctcccgcgt tcgcccaggc
     2161 aacgccgaag aaaatgcaga tcgccgcggt caatccggtg ccggaatcgg gtgcgatcgc
     2221 gctggattgg atggccaagg cactgactga acggaccaag ggcgagctgg acgtcaccta
     2281 tcatggcggc acgctgctga ccaaagagat cgacatcatg aacgcggtga agggcggcaa
     2341 catcgccatg ggcaatcccg gcggcgcttc ggcaaccttg ttcccggaga tgggcgtctt
     2401 cctggtcggc tacctgatca gcagctacca gcaggcctac gcgatcatga acggcaaggt
     2461 gggcgacgac ctggacaagc tgtttcagca gaaatacggc gtaaaggtca ttttctactt
     2521 cgactatggc ttccggcatt tctggaattc caagcgggca atcaacgtgc cgaaggattt
     2581 gcgcggcctg aaactgcgct cgcagccgtc caaggtgttc gccgacaccg tcaacagcct
     2641 gggcggcgtg gctgtcccgt tgggttgggc cgaggtcatc accgcggcac agcagggcgt
     2701 catcgacggc gccgacctgc cggtcgtcaa catggtgcct ttgaaggcct acgaggtgtc
     2761 caaatactac tcgctgaccg ggcacaacta cggcgccacg ttgatcgcca tgaacctgca
     2821 aatgtggaac gacctgcgtc cagagcagca gcgactggtg cttgataccg gccgcgaggc
     2881 gcagggcgtc gtgcgcaagg tcaccgagga cgtggataca ctggcaagcg ccaaggagca
     2941 gcttgaatcc cgtggcatga tcgtcaatca gccggatccg gcaccgttca tcgacatcgc
     3001 gaaacagaaa ctttggccgc aatacgagaa gcaatacagc gggctgtggg agcaaatcgt
     3061 ctccaccaag gtttgacgcg gatgcggctt ctgacgttgc tgcccagggt tttcctgggc
     3121 gggctcgtgc tattcgctat ttcggtcatg ctctacggcg ttttcgcccg atacgtgctg
     3181 ctgccgatca ccgactggct ggatatcgat cagcccaact tcttctgggt ggaggaagtc
     3241 ggtgaaacca cgctggcctg gctgaccatg atcggggcgg cgatcgcggt gaaagagcgg
     3301 agccattttg ccttgaacct cgtgacccat cgtttctcca cgcgtgccaa ggccgtgctg
     3361 catgtggccc ataacatgct cattgcgttg ttcggcgggc tggttgcctg gcttgggttc
     3421 aagctggtgc tgctgaattc catgctgacc tccccggcgt tggaaatgag cctggggtgg
     3481 ctgtatggct ccaccgtcgc cggcggcatc ctgatcgtgc tttacgccct agatctggcg
     3541 agaaagccgg cgggtccgga gcattccttc gccgacgtgc gggagtagcc agcgtggtcc
     3601 tgttcgcaac attggtcacg ttcctgatct tgctggtcct gtcgttgccg atcgtgttct
     3661 gcctgggcgc ggcgggggtc atcggattgc tgaccgcggg tctgccgctg cagcagcttg
     3721 gatcgagcat cgtgtcggcc tcgcaaagct gggtgctgct ggccattcca tcgttcgtct
     3781 tcgccggctc gctgatggaa agggcaggaa tgtccaccgc gctggtggaa ctcgcccgag
     3841 cgctggtggg gtggctgcga ggcgggttgg gaatgtcggt gatcgtggtg gcgtatttct
     3901 tctccgacat ctgcggctcg aagatggcag aggtgtcggc gcttggctct gcgctgatgc
     3961 cgtcactgaa gaaagccggc taccgcccgg aagatggggc atcgctgatc gccgccggca
     4021 ccgccatggg gatgctggtg ccgccggcga tcttcatgat cgtgatcagt gcggtgacca
     4081 atcagtccgc ggtggcgctg ttcctggccg gcttcatccc ggcggcgacc attggcgcct
     4141 gcctgtgcgt cctggtactg atccaggcgc atgtgctgaa ctggccaaag gacacccgca
     4201 ccagccttgc tcggttgctg acggccctga agaacgccgc ggtgccgctg gtgatcccgg
     4261 tcgtgatcct gggcggcttc tacatgggcg cgttcaccgc gaccgaggct ggggcgatcg
     4321 tcgcgctgta ttcgctgctg gcagcgaagt tctattaccg caatatcact tggatgcagg
     4381 tgctggtcct ggcgtacgag gccggcgtgc tgaccggcgt cgtcgttttc ctgttggccg
     4441 tggcgacaat cttccaatac ctgatgggcc tgtccggcat gccggagttc ctcggttggg
     4501 cgctcggtcc gctggagaaa accccgtggc tgttcctgac cggcgtcgcg ctgctgacgc
     4561 tggtattcgg catggtgctg gaaggactgc cggccgccgt cgtgctgatc ccggtggtgt
     4621 tcccaatcgc cacgaagatt ggaatccatc cggtgcactt cgatatcgtc cagaccgccg
     4681 ctgtcggtat cgggttgttc accccgccgc tgggcgtggg tttgctgatg gcgctgcggt
     4741 tcgcggatgt ctcggtgggg cagcatgcga aggcctactg gccttatctg ctggcgctgg
     4801 tgatcggact gatgctgatc atatgcattc cggagttatc cttgttcctg ccccggagcg
     4861 cgggcttgat ccggtagtcg tcgggt
//
LOCUS       DASWQN010000286         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_161228, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000286 DASWQN010000000
VERSION     DASWQN010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161228"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VGC82_04760"
     CDS             <1..1258
                     /locus_tag="VGC82_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY0422616.1"
                     /translation="RNRPGQGGGDNQPSARRAQSLGSGFIVDASGLVVTNNHVIEGAD
                     EVSVTLQDNTTLKATVVGRDESGDIALLKVQTDKSLPTVDFGDSTQSRVGDWVLAIGN
                     PFGLGGTVTAGIVSARGRDIHQGQYDDFIQTDAAINRGNSGGPLFNMDGQVIGINTAI
                     FSPSGGSIGIGFAIPSNMAKNIVAQLKEFGHPRRGWLGVKIQQVTPEIAESLGLKDAS
                     GAMVAGVSDGGPAEKAKIRGGDIILKFGGQDVKEMHNLPRIVADAEVGKAVPVILWRE
                     GKEITVQTVLAERPADAQLASVDATKPVDTTRPTDISGLGLKVAPVSQELKDKFQLGA
                     EQKGVVITDVAPNTPAAERGLKPGDVIVEVQQTEVAAPSDVQRQVDIARKADRKNVLV
                     LIQREGGVQYVPLSLPKVGGKDKQPG"
     gene            1314..>1735
                     /locus_tag="VGC82_04765"
     CDS             1314..>1735
                     /locus_tag="VGC82_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0422617.1"
                     /translation="MSWSAQQYTSFERERTRPVRDLVQAIEPSVVARAVDIGCGPGNS
                     TEVLAARFPSAAISGLDSSPDMIEAARKRLPAFRFDLAGIQDWADPGPFDVILANAVL
                     QWVPDHASLMPALMEKLAPGGNLAVQMPDNLEEPAHRL"
BASE COUNT          338 a          536 c          571 g          290 t
ORIGIN      
        1 tcgcaaccgg ccgggccaag gcggcggcga taatcaaccg tcggcgcgcc gtgcgcaaag
       61 cctcggctcc ggcttcatcg tcgatgcgtc gggtttggtg gtcaccaaca accacgtcat
      121 cgaaggtgcc gacgaggttt ccgtcacctt gcaggataat acgacgttga aagccacggt
      181 tgtcggccgc gatgaaagcg gcgacatcgc gctgctgaag gtgcagacgg acaagtcgtt
      241 gccaaccgtc gacttcggcg attcaaccca gtcccgcgtc ggcgactggg tcctggccat
      301 cgggaacccg ttcggcctgg gcggcaccgt gaccgcgggc atcgtttcgg cgcggggccg
      361 agacatccat cagggccagt acgacgactt catccagacc gacgcggcga ttaaccgcgg
      421 caactccggt ggcccgctgt tcaacatgga cggccaggtg atcggtatca acacggcgat
      481 tttctcgccg tcgggcggct cgatcggcat cggctttgcc attccctcca acatggcgaa
      541 gaacatcgtt gcccagttaa aggaattcgg ccatccccgc cgcggctggc tgggcgtaaa
      601 aatccagcag gtcacgccgg agatcgccga gagcctgggg ctgaaagatg cctccggtgc
      661 gatggtcgcg ggcgtcagcg atggcggccc cgcggaaaaa gccaagatcc gcggcggcga
      721 catcatcctg aagttcggcg gccaggacgt caaagagatg cacaaccttc cgcgcatcgt
      781 ggcggacgcg gaagtcggca aagccgtgcc ggtgatccta tggcgtgagg gcaaggaaat
      841 taccgttcag acggtcctgg cggaacgccc ggcggacgca cagctcgcgt cggttgacgc
      901 caccaagccg gtggacacca ccaggcccac cgacatctcc ggcttgggac tgaaagtggc
      961 acctgtcagc caggagctga aggataagtt ccagctcggt gccgaacaga aaggtgtcgt
     1021 gatcaccgat gtcgctccga acacgccggc cgccgaacgc ggcctgaagc cgggcgatgt
     1081 catcgtcgag gtacagcaaa ccgaggtcgc ggcgccgtcc gacgtgcaaa ggcaggtcga
     1141 tatcgcacgc aaggcggatc gcaagaacgt cctcgtactg atccagcgtg agggcggcgt
     1201 gcaatatgtg ccgctatcgc tgcccaaggt cggcgggaag gacaaacagc cgggctaagc
     1261 agtatcgtgg gtcggatcac cctggcttgg cgtcatccaa gcggaggcca gtcatgagtt
     1321 ggtcggccca gcaatatacc agcttcgagc gggagcgtac tcgtccggtg cgggacctgg
     1381 tccaggcgat cgaaccatcg gtcgtcgcgc gggctgtcga catcggctgc gggccaggca
     1441 actcgaccga ggtgctggcg gcacgtttcc cctccgctgc gatatccggc ctcgacagtt
     1501 cgccggacat gatcgaggct gcccgaaagc gcctgccggc attccgtttc gacctggcgg
     1561 gaatccagga ctgggccgat cctggcccgt tcgacgtgat cctggccaat gcggtcctgc
     1621 agtgggtccc tgatcacgcg tccctgatgc cggctttgat ggaaaagttg gctcctggtg
     1681 ggaatctggc agtacagatg cctgacaacc tggaggagcc ggcgcaccga ttgat
//
LOCUS       DASWQN010000287         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2140601, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000287 DASWQN010000000
VERSION     DASWQN010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140601"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="VGC82_04770"
     CDS             complement(<1..1646)
                     /locus_tag="VGC82_04770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0422618.1"
                     /translation="MNRLLAAVVDPGWYAMRYPEVAAAGQDAVSHFVRFGAAERRDPN
                     RFFDGTWYLAHYPDVAASGMPPLLHYLQAGAAELRDPHPRFDAPFYVRRHPDAAGNPL
                     LYHLRIGLRRGYPTEQSINIRDYLPSHNPALLGVPEVSADVVIPVCSGDTQTRDTIES
                     VLADRPLPPARIIVVDDDLGEPPLSAWLQELAAGGHIHLLRNPRKLGFAACAKQGIDI
                     AATHDVVLLRSGTVVFPGWLSRLAAHAASDPHIATVSPLCATVAAGVASLGLAPALLD
                     EVCRSVNTARSVPAGAPAGNCTYIRRDALRQVGGMTDGAGPEADFCRRAAEAGWQNRL
                     AYDIFVHGQLVHGQGLANPEDYPLPADNPTDGVATDPFEFALAAGLFRRSNLPVILMI
                     SHAMGGGVRRHIEGLVKRYRRAAHIVLLQEAVRGVALSLPALSDQPVLKLPSDRLDDL
                     VLVLKSANLSRVHVHHQLHLDIDIRRLIHRLDLPFDVTVHDYYAICPQINLLPWPEGI
                     YCGEPSPSGCNACIADNSSHGAKDIVSWRREKAWQFIDADR"
BASE COUNT          341 a          525 c          573 g          299 t
ORIGIN      
        1 cggtctgcgt cgatgaactg ccatgctttc tcgcggcgcc aggacacgat gtctttggca
       61 ccgtgcgaac tgttgtcggc aatgcacgcg ttgcagccgg aaggggatgg ttcgccacaa
      121 tagatacctt cgggccatgg cagcaggttt atctgcggac agatcgcgta gtagtcatgc
      181 acggtcacat cgaacggcaa atcaagccga tggatcaaac ggcggatatc gatgtcgaga
      241 tggagctgat ggtggacatg caccctggac aggttcgctg atttcagcac caggaccaga
      301 tcatccaggc ggtcggacgg cagtttcagc actggctggt ccgacagcgc cggcagcgaa
      361 agcgcgacgc cgcgaaccgc ttcctgtaac aggacgatgt gcgcggctcg tcgataacgt
      421 ttcaccagtc cctcgatgtg ccgcctgacc ccgccgccca tggcgtgcga gatcatcagg
      481 atcaccggca ggttcgaccg gcgaaacagc cccgccgcca gggcgaactc gaagggatcg
      541 gtggcgacac cgtcagtcgg attgtccgca ggaagcggat aatcttcggg attggcaagg
      601 ccctggccat gaacgagttg gccatgaacg aagatgtcgt aggcaagccg attctgccac
      661 ccggcttcgg ccgctcggcg gcagaaatcg gcttccggtc ccgcgccgtc agtcatgcct
      721 cccacctggc gcagcgcgtc gcggcggatg tatgtgcagt tgcctgccgg cgcccctgcc
      781 ggcaccgatc gggcggtgtt tacggaacgg cagacctcat cgagcagtgc cggagccagt
      841 ccaaggctgg caacgcccgc ggcgacggtg gcgcataagg gcgagaccgt cgcaatgtgt
      901 ggatccgatg cggcgtgggc agccagtcga gacagccatc cgggaaacac caccgttccg
      961 ctgcgcagca agacgacgtc gtgagtggcg gcgatatcga tgccttgttt ggcgcaggcg
     1021 gcaaacccga gtttgcgcgg gttgcgaagc agatggatgt ggccgcccgc ggccagttcc
     1081 tgaagccacg ccgacaaagg cggttcaccc aagtcatcgt ccaccacgat gatgcgggcg
     1141 ggcggaagcg gccggtccgc gaggacggac tcgatggtat ccctggtttg cgtatcgccg
     1201 gaacagaccg gaatgacaac gtcggccgat acttcgggca cccccagcaa ggctggatta
     1261 tgcgatggca aatagtcgcg gatattgatc gattgttccg tcgggtaccc gcgtctcagg
     1321 ccgatccgca gatggtacaa caacggattg ccggcggcat ccgggtgccg gcgcacgtag
     1381 aacggggcgt cgaaacgtgg atgcggatcg cgcagttcgg ccgcgcccgc ttgcaggtaa
     1441 tgcaacagcg ggggcatccc gctcgcggca acgtccggat aatgcgccag gtaccaagta
     1501 ccgtcgaaga accggttggg atcgcgcctt tccgcagcgc cgaagcgaac gaagtgcgac
     1561 accgcgtcct ggccggcggc ggcgacctcc gggtagcgca tcgcgtacca acccggatcg
     1621 accaccgcgg ccagcaggcg gttcaacttt tcggccggcg aggcggtttt agagcctgtt
     1681 tgagtgcaat tgtgctcctc cgtccgcatc ttaatctcag tgattgaaga agaggtcc
//
LOCUS       DASWQN010000288         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_557034, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000288 DASWQN010000000
VERSION     DASWQN010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557034"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VGC82_04775"
     CDS             complement(<1..598)
                     /locus_tag="VGC82_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0422619.1"
                     /translation="MFRLDGKAALVTGASGGIGAAIARALHAQGANVVLSGTRRDALE
                     ALAVHLGERAHVCPADLRDASEPDALVEAAEKAAGPLHILVNNAGMTRDMLALRMSDQ
                     DWQAVLDVDLSAPFRLIRAALRGMLRRRAGRIVNISSIVGATGNAGQANYAAAKAGLV
                     GMSKALAQEVASRGITINVVAPGFVVTGMTDVLGEGQKA"
     gene            complement(618..1580)
                     /gene="fabD"
                     /locus_tag="VGC82_04780"
     CDS             complement(618..1580)
                     /gene="fabD"
                     /locus_tag="VGC82_04780"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY0422620.1"
                     /translation="MPVYAFIFPGQGSQAVGMGRDLAAAFAAAREVFQEVDETLKQKL
                     SKLMFEGPHEELTLTENTQPALMAHSMAVLRVLEREGGTTLQRKALVVAGHSLGEYSA
                     LAASGAFTVQAAARLLKLRGQAMQKAVPAGIGAMAALLGAAMDQAQKICAQAGPVPET
                     KILEVVQPANDNGGGQVVISGHRSAVERAIEIAKATGIKRAMLLPVSAPFHCALMAPA
                     ADAMAEALEATPPGAPLVPLVANVSAKRESDPQRIRDLLIQQVTATVRWRECVSAMTD
                     MGVDRFIELGAGKVLTGLIKRIEPDAAGFAVGTPAEIEAVLKSL"
     gene            1660..>1918
                     /locus_tag="VGC82_04785"
     CDS             1660..>1918
                     /locus_tag="VGC82_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422621.1"
                     /translation="MGIRTVKEQAGLSARGRGGFGSVETEVGGTARSGNPAEPNNIML
                     GQLYRSDMLSLIFETARKGCTGAASLDHPWKVSQIAIFARHG"
BASE COUNT          347 a          649 c          568 g          354 t
ORIGIN      
        1 tcgccttttg cccttcacct aggacatcgg tcatccccgt gaccacgaag cccggcgcca
       61 cgacgttgat ggtaatgccg cgggaagcga cttcctgcgc cagtgccttg ctcatgccca
      121 ccagtcccgc cttcgcggca gcataattgg cctggccggc attgccggtg gcacccacga
      181 tcgaggaaat attgacgatc cgaccggccc gccgccgcag catgccgcgc aacgccgccc
      241 ggatcaggcg gaacggcgca gacaaatcga cgtccagcac cgcctgccaa tcctggtccg
      301 acatacgcag cgccagcata tcgcgggtca ttccggcgtt attcaccaat atatgcagtg
      361 gcccagcggc cttctcggcc gcctcgacca acgcatcggg ttccgaggca tcgcgcagat
      421 cggccgggca aacatgggca cgctcgccga gatgcacggc cagagcctcc agcgcatcgc
      481 gccgcgtgcc agacagcacg acgttggccc cctgggcgtg cagcgcgcga gcgatggccg
      541 ccccaatccc gcccgaggct ccggtaacca gcgcggcctt gccgtccaat cggaacatcg
      601 ccgcttcctt tcttcggtca gaggcttttg agaaccgctt caatttccgc cggcgtgccg
      661 accgcgaacc cggccgcatc cggttcgatc cgcttgatca gcccagtcag taccttgccg
      721 gcgccaagtt ctataaaccg atcgaccccc atatccgtca tcgccgaaac gcattcgcgc
      781 caacgcacgg tggctgtcac ctgctgtatc agcaggtcgc gaattcgctg gggatcggat
      841 tcgcgtttgg ccgagacatt cgccaccagc ggcaccagcg gggcacctgg tggcgtggct
      901 tccagcgctt cagccatcgc atccgcggcc ggagccatca gcgcgcaatg gaacggagcg
      961 gacaccggca gcagcatcgc tcgtttgatg cccgtggcct tggcgatctc aatcgctcgt
     1021 tccaccgccg aacgatgtcc ggagatcacc acctgcccgc caccgttgtc gttggccggc
     1081 tgcacgactt ccaagatttt ggtttctggt accggaccgg cctgggcgca aatcttttgc
     1141 gcctgatcca tggcggctcc taataacgcc gccatcgccc caatgcccgc cggtaccgcc
     1201 ttctgcatcg cctggccgcg cagtttcagc agccgcgcgg cagcctgtac ggtgaaggca
     1261 cccgaagctg ccagtgcgct gtattcaccc agcgaatgac cggccaccac cagggccttg
     1321 cgttgcaacg ttgtgccgcc ttcccgttcc agcacccgca gcacggccat cgaatgcgcc
     1381 ataagtgccg gctgggtgtt ttcggtcagc gtcagttctt cgtgcggccc ttcgaacatc
     1441 agcttcgaca gcttctgctt cagggtctca tcgacttcct gaaacacctc ccgcgcggcg
     1501 gcgaacgcag cggcgaggtc gcggcccata ccaacggcct ggctgccctg gccggggaag
     1561 atgaaggcgt aaaccggcat ggcgcgttat ctctccggga tgtcctgcga cggacgaaag
     1621 ggattggccg acgaatagga ttggcgcggc ggcgtagccg tgggtatccg gactgtcaag
     1681 gaacaggccg gcttgtctgc acggggacgg ggcggtttcg ggtcggtaga gactgaagtc
     1741 ggcggcacgg caaggagcgg caacccggcc gaaccaaata acattatgct tggccaatta
     1801 taccgttctg atatgttaag cttgattttt gagacggcgc ggaaaggttg taccggtgcg
     1861 gcaagccttg accatccatg gaaggtttcg caaatcgcga tctttgctcg tcatggca
//
LOCUS       DASWQN010000289         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1217071, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000289 DASWQN010000000
VERSION     DASWQN010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217071"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VGC82_04790"
     CDS             complement(<1..191)
                     /locus_tag="VGC82_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0422622.1"
                     /translation="MDLTGKRVFLVEDEALVSMMFEHFLEDLGCAIVGTAARLDDALD
                     IGRTLAMDVAILDINLNRQL"
     gene            732..1295
                     /locus_tag="VGC82_04795"
     CDS             732..1295
                     /locus_tag="VGC82_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0422623.1"
                     /translation="MVSQLRQVWNDHRLRRQLRELLPALNKFALKLTQSKHDAEDLVQ
                     MACEQALITLPMALPDDQMRSELQGSMRTLWSGELRGRDIQDRHVRDEQVNPNRASAE
                     DGEGLADNRALLQDLEHAILRLPEGERCMLQLICVQGFSYKKAADIAGVPIGTVMSRL
                     SRARLHLMEEISLDRYASNGNVRTLRP"
     gene            1292..2065
                     /locus_tag="VGC82_04800"
     CDS             1292..2065
                     /locus_tag="VGC82_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY0422624.1"
                     /translation="MTVPDDMTLMAFADGELDQATARDVSEFIARDLDAQDKVRQFQQ
                     SADLVRAVFAQPHFRQPSPVRRKVRRSPVAFAPFSRPRYSLIAASLALFLVGMGAGAG
                     VILVNPGPSFSERLLDEVADYHTLYAREAEHQVEVPANRLAHIETWLGNRLHRPLAVP
                     DLSARGLTFVGARLLSVDGAPVAQLLYHAPGREHQPVALCIAADKTPDAAVRGEEYSG
                     VHEVTWSRGGYSYVIAGWETNDFLVSLAAELEPKFELSL"
BASE COUNT          440 a          646 c          741 g          469 t
ORIGIN      
        1 agttgcctgt tcaggtttat gtccaggatc gcgacatcca ttgccaatgt gcggccgatg
       61 tccagcgcgt catccaggcg cgcggccgtg ccaacgatgg cgcatcccag gtcttccagg
      121 aaatgctcga acatcatcga aacgagcgct tcgtcttcca ccaggaagac ccgttttccg
      181 gtcagatcca cgatagatcc ttcctgtgcc gccgacatga aggaacctcg gcatctccgc
      241 ctcggcggtt atcggagccg cgctgtttcc aagtgggttc gtcgccagtt ggttctgtgc
      301 agcgacgcag gtggccgccc gccgaactgc agacggccaa tggtgtgtcc gtgtgtaaat
      361 cttgcaagtc acgatcctta tcggccgtct taatccgcga cccggtggaa gccgtgcttg
      421 tcagccgtat tcgttgctta gtcgggtggt tatcgatacc gggaagtcag gcgtatcgcc
      481 tgcacgccat cctgtagccg atttctcagg gcgaatgaca gaaggttcta cggctatccg
      541 aaagaacggt tgcgaccggt ctcggatgtg agttttctgc aacaaatatc accaacacgg
      601 tgttgtcatt tttaacttat cgaaatgaat atataacccg gttgtcgtct gtcgaagccg
      661 cattggcgtg gcagcggatt gttggaaaca aaggcggaca ttggtccgcc gcgaacagat
      721 cgacgggtag catggtttcg cagctcagac aggtatggaa cgatcatcgc ctgcgccggc
      781 agctccgcga gttgctgccg gcgctgaaca agttcgcgct gaagttgacc caatcgaagc
      841 acgacgccga agatctggtg cagatggcgt gcgagcaggc gttgataacg cttccgatgg
      901 cgctgcccga cgatcaaatg cggagcgaac tgcagggctc gatgcggaca ttatggagcg
      961 gggaacttcg cgggcgcgac attcaggatc gccatgtgcg ggacgaacag gtcaatccca
     1021 accgggcgtc ggcggaggat ggcgaagggt tggcggacaa ccgggcgctg ttgcaggatc
     1081 tggagcacgc catcctccgt ctcccggagg gcgagcgctg catgctgcaa ttgatctgcg
     1141 tacagggctt tagctacaaa aaggccgcgg acatcgccgg cgtaccgatc ggcacggtca
     1201 tgagccgcct ttcccgcgcg cgattgcatc tcatggagga gatttcgttg gaccggtatg
     1261 cttcaaacgg caatgtaagg acgctgcggc catgacagtc ccggacgaca tgaccttgat
     1321 ggcgttcgcc gatggcgaac tcgaccaagc gaccgcgcgg gacgtttcgg agttcattgc
     1381 ccgggatctg gatgcgcaag acaaggtaag gcagtttcag caatccgccg atctggttcg
     1441 ggcggttttc gcgcagccgc atttccgcca gccatcgccg gttcggcgca aggtccgccg
     1501 gtcgcctgtg gcgttcgcgc cattttcccg accgcgatat agcttgatag cggcttcgtt
     1561 ggctttgttc ctggtcggca tgggggcagg ggcaggagtc atcctggtca acccgggccc
     1621 gagcttcagc gaacgattgc tcgacgaggt cgccgactac catacgctgt acgcacgtga
     1681 ggccgaacat caggtcgaag ttccggcgaa ccggctcgcc catatcgaga cctggcttgg
     1741 gaaccgcctt cacaggccgt tggcggttcc ggatctttcg gcgcgtgggc tgacgttcgt
     1801 cggagcccga ctgctctcgg tggacggagc acccgtggca cagctcttgt accacgcgcc
     1861 tgggcgcgag caccagccgg tggccctttg cattgcagcg gacaagaccc cggatgcggc
     1921 cgttcggggt gaggagtatt ccggggtgca tgaagtcaca tggagccgtg gaggctacag
     1981 ctacgttatc gccggttggg aaaccaacga cttcctggtc tctcttgccg ctgaactgga
     2041 accaaagttc gagttgagct tgtagggaac ggacctctcc ggctgacggt gttctggcgg
     2101 tcggggctgg gatcgggccg taatagcggt tgatgttatt gccgtgctag ctggcaaccc
     2161 gcgaccccgt tgatgcggga aaatcgtggg tggccgcgcc aagcacggcc atgacacagg
     2221 ggggatgacc ctggcacagg ggggatgacc ctggcacagg ggatcgggac cagaagctta
     2281 tgacggagcg tggatg
//
LOCUS       DASWQN010000290         6260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_425305, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000290 DASWQN010000000
VERSION     DASWQN010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425305"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2727
                     /locus_tag="VGC82_04805"
     CDS             <1..2727
                     /locus_tag="VGC82_04805"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010706.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEY0422625.1"
                     /translation="PSALFARGLSRLSNLSPADKRPAIAAGLRQAKRTTALVAAIADV
                     GGIWSLEAVTGALSDLAEAALRLAIGHLLRSAHDAREIRLQDANRPEVNCGFVALAMG
                     KLGAAELNYSSDIDLLLIYDATAPVYAAGRAADSIGAFTSRLARDLITLMEKRDADGY
                     VFRTDLRLRPDPAATPPAVSLHAALTYYESMGQNWERAAMIKARPIAGDLALGHRFLE
                     AIRPFVWRRGLDFAAVADIHAMKRRIDVAKKTALGRGSDPVARIAGHNVKLGEGGIRE
                     IEFLVQTLQMVWGGRDPKLRVKSTLPAMVRLVATGHLDEAARRQLDASYRYLRQVEHR
                     IQMVNDRQTHNLPEQPPEMERIARFMGYPDAVAFAGDFLSHVDVVRANYRTLFEHVPD
                     LPGTEVVGPELDFRGDDPQPAATVAALNTLGYHDAQHIIAAVRRWLAGRVRALRSTRA
                     RDLMTTMVPAVLVALGRQANPDEAFNRFDRFISALPAGVQPMSLFQHNPALLDRVATV
                     LGAAPMLSEHLARHPNALEGLLSSQDAVDAGRMLRLRISDATKLEDVIQIARRAVKER
                     DFYLSVATLEGWLDVNGSGQQRTALAEAALATLVPRVLADFATRFGRVEGGALAVVAM
                     GKAGGREMMAGSDLDLMFIYDHPADVAESRGARSLPSSQWFVRAVQACIAALTAPGPE
                     GQMYELDMRLRPSGNKGPLAVSLESFKRYHAMDAWTWERMALTRARVVAGSADMRRRV
                     NQAINVGIRREQEPDQIRANATSMRSRMARDLRPHGPWDVKLRTGGLIDIEFIAQALQ
                     LVHVREPHFFHSQTTRVALRRLSKAGVLGRADARLLIDAEHLWRTIQGMLRMTVGRVE
                     TGSLPDASALPLLRAAAKAGIAAVDTDDLLRKIDDIAKDVRSLFERYIGKPV"
     gene            2727..3200
                     /gene="bcp"
                     /locus_tag="VGC82_04810"
     CDS             2727..3200
                     /gene="bcp"
                     /locus_tag="VGC82_04810"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEY0422626.1"
                     /translation="MSLMEGDAAPDFQIAATNGRTVSLGAYKGKPLLLYFYPKADTPG
                     CTKQACAFQEATPRLEEIGLDVIGVSPDKIKPIEKFAAKYGLLFPLASDEDHAVAERY
                     GTWVEKSMYGRKYMGMERSTFLIGGDGKVLKIWRKVSVTGHADDVLKAARSLKLV"
     gene            complement(3201..4610)
                     /locus_tag="VGC82_04815"
     CDS             complement(3201..4610)
                     /locus_tag="VGC82_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0422627.1"
                     /translation="MPLNITLDTQQKDPLFLQIAVRVRTAIAAGQLLPGAKLPSSRAL
                     AAQLAIARGTVDAAYALLAGEGAIETRRSAGTIVSGPVGRRIEVAEQSPLLFPGSPPT
                     EAAGARPFQMGLPSLDAFPRKRWSNLTVQAARSLQPADLAEADPSGTPALRSEVAAYL
                     GIARGVRCSPEQVLITGGYQGALALIRNVLLRPGDPVWMEDPGYHMTRLALETAGARV
                     VPLRVDGEGLRVAAGIKAAPKARLAVVTPTHQCPTGVSLSLPRRMELLAWASEAESWI
                     VEDDYDSEFRYVGRPLPSLKSLDRGQRVLYAGSFSKVLFPALRLGYLVVPPELAKAFQ
                     RASRSLTSGQPVLEQKVVAAFMRNGHFARHIRRMRLLYAERRRCLTAALKATFEDRVT
                     VELAPGGMHLLARFPNAADDTVLAKRSVQIGLAPTALSSLAMAHDAGQGLLLGFTNIV
                     PAEASSLVARLASVIDRPA"
     gene            complement(4808..>6260)
                     /gene="acs"
                     /locus_tag="VGC82_04820"
     CDS             complement(4808..>6260)
                     /gene="acs"
                     /locus_tag="VGC82_04820"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY0422628.1"
                     /translation="FGGFSPDSLANRIQDCDSTLLITADEGRRGGRSVALKANADAAL
                     KDCPSVKNVIVVKVTGASVHHEPGRDLDYGALLAAASPDCPPEPMSAEDPLFILYTSG
                     STGKPKGVLHTTGGYLVWASYTHEKVFDYRPGEVYWCPADVGWVTGHTYILYGPLANG
                     ATTLMFEGIPNYPDNSRFWQVIDKHKVSIFYTAPTAIRALMRNGDGPVKETSRASLRL
                     LGSVGEPINPEAWLWYYNVVGEKRCPIVDTWWQTETGGILISPLPGATALKPGSATLP
                     LPGVQPLLVDGDGNELDGATEGNLCIRESWPGMMRTVFGDHERFVQTYFSTFKGLYFT
                     GDGARRDADGYYWITGRVDDVINVSGHRMGTAEVESALVAHPKVAEAAVVGFPHDIKG
                     QGIYAYVTLNADEEPTEALRKELIAWVRKEIGPIASPDAIQWAPGLPKTRSGKIMRRI
                     LRKIAANETDSLGDISTLADPGVVTELVENRVG"
BASE COUNT         1144 a         1992 c         2005 g         1119 t
ORIGIN      
        1 ccctccgccc tgttcgcgcg tggcctgtcc cgccttagca atctttcccc ggccgacaag
       61 cgccccgcca tcgccgcggg tcttcggcag gcgaaaagga cgaccgccct ggtcgccgcc
      121 atagctgatg tgggtgggat ctggtcgctg gaagccgtca ccggcgcgct gtccgacctg
      181 gccgaagctg cccttcgttt ggcgatcggc cacctgctgc ggtccgccca tgatgccagg
      241 gaaatccgcc tgcaggacgc caaccggcct gaggtcaatt gcgggtttgt cgcgctggca
      301 atgggaaagc tgggcgcggc ggagctgaac tattcgtccg atatcgatct ccttcttatc
      361 tatgacgcca ccgcgccggt ttacgccgct ggacgcgccg ccgatagcat tggcgcgttc
      421 acgtcccggt tggcccgcga cctcattacc ttgatggaga aacgcgacgc ggatggatac
      481 gttttccgca ccgacctgcg actgcggccg gaccctgcgg cgacgccgcc cgcggtctcg
      541 ctgcacgcgg cgctgactta ttacgagagc atgggtcaga actgggagcg cgcggccatg
      601 atcaaagcgc gtcccatcgc tggcgatctt gcgctgggtc accgctttct ggaggcaatc
      661 cgcccgttcg tgtggcggcg cgggctggac ttcgccgccg tggccgacat ccatgcgatg
      721 aagcgacgca tcgatgttgc aaagaaaacg gcactgggac ggggaagcga tccggtcgcg
      781 cgcattgccg gtcacaatgt caagctgggt gagggcggca tccgggaaat cgagttcctg
      841 gtgcagactc tgcaaatggt gtggggcggc cgcgacccta agctccgggt caaatcaaca
      901 ctgccggcga tggttcgact ggtagcgacc ggccacctgg acgaagccgc ccggcggcaa
      961 ctggatgcct cgtaccggta ccttcgccag gtcgagcacc ggatccagat ggtgaacgac
     1021 cgccagacgc acaatctgcc ggaacaaccg cccgaaatgg agcgaatcgc gcgcttcatg
     1081 gggtacccgg atgcggtcgc cttcgccggc gacttcctgt ctcatgtcga cgtcgtccgg
     1141 gcgaattacc gaaccctgtt cgagcacgta ccggatctgc ctggtactga ggtcgtcggg
     1201 ccggaactgg attttcgcgg tgacgacccg cagccggcag cgaccgtcgc ggcgctgaac
     1261 actcttggat accacgacgc gcagcatatc atagcagcgg ttcggcggtg gctcgccggg
     1321 cgggtgcgtg cattgcggtc aacccgagcg cgggatttga tgacgaccat ggtgccggcg
     1381 gtcctggttg ctctggggcg ccaggccaat cccgacgagg cgttcaaccg gtttgaccgg
     1441 ttcatcagcg cattgccggc cggcgtgcag cccatgtcgc tgtttcagca taatcccgcg
     1501 ctgctggatc gggtcgccac ggttctcggc gcggcgccca tgctgtcgga acacttggcg
     1561 cgccacccca acgcgctgga gggtttgctc tcgagccagg acgcggtcga tgccgggcgc
     1621 atgctccgtt tgcgcataag cgatgcgacc aagctggagg atgttatcca gatcgcccga
     1681 cgggccgtaa aggagcgcga tttttacctc tccgttgcca ccctggaggg ttggctggac
     1741 gtcaacggtt ccggtcaaca gcgcacggca ctggcggagg cggcattggc gacactggtg
     1801 ccgcgcgtgc tggcggactt cgccacacgt tttggccggg tcgaaggcgg cgcactcgcg
     1861 gtcgtggcga tgggcaaggc cggcggacgc gagatgatgg cgggctccga cctcgacctg
     1921 atgttcatct atgaccatcc ggccgatgtc gccgaaagcc gcggagcacg cagcctgccc
     1981 agcagccaat ggttcgtccg ggccgtgcag gcctgtatcg ccgcgctgac tgcaccgggg
     2041 ccggaagggc aaatgtacga gctggatatg cgcctgcggc cgtcgggcaa caaagggccg
     2101 ctggcggttt cgctggaaag tttcaaacgc taccacgcga tggatgcctg gacgtgggaa
     2161 cgcatggcgc tgactcgcgc ccgcgtcgtt gccggctccg cggatatgcg ccgccgggtg
     2221 aaccaagcca ttaacgtggg catcaggcgg gaacaggaac ccgaccagat ccgcgccaac
     2281 gcgacgtcga tgcgttcccg gatggcccgc gaccttcgcc cgcacggccc ctgggacgta
     2341 aaacttcgca ccggcggact catcgacatc gagtttatcg cccaggcgtt gcaattggtg
     2401 catgttcgtg aaccccactt ttttcatagc cagacgacac gcgtcgcgct gcgccggcta
     2461 agcaaggcag gcgtgctggg ccgagcagac gctcgtttgc tgatcgatgc cgaacatctt
     2521 tggcgcacca tccagggcat gctgcggatg accgtcgggc gcgtcgagac aggctccctg
     2581 ccggacgcct cggccttgcc gctgctgcgt gccgccgcga aggcaggcat cgccgcggtt
     2641 gacactgatg atctgttgcg aaagatagat gatatcgcga aagacgtcag aagcctgttt
     2701 gaacgctata tagggaaacc agtctgatga gtctcatgga aggtgatgcc gccccggact
     2761 tccagattgc cgccaccaat gggcgaacgg tcagcctggg cgcctacaag ggcaaacctc
     2821 tgctgttgta tttctacccg aaggccgaca cccccggctg caccaagcaa gcttgcgcct
     2881 tccaggaggc cacgccgcgc ctggaggaga ttggactgga tgtcatcggt gtttcgccgg
     2941 acaagatcaa accgatcgag aagttcgccg ccaaatatgg cctgttgttt ccattggcct
     3001 ccgacgagga tcatgcggta gccgaacgtt acggcacatg ggtcgagaag tcgatgtacg
     3061 gacggaaata tatgggcatg gagcgcagca cgttcctgat cggcggggac ggcaaggtgc
     3121 tgaagatatg gcggaaggtc agcgtgactg gccacgcgga cgatgtcctg aaggcggcgc
     3181 gcagtcttaa gctggtgtga tcacgccggc cggtcgatga cgctggcgag ccgggccacc
     3241 agcgaactgg cttcggccgg gacgatattg gtaaagccca gcaacaagcc ctgaccggcg
     3301 tcgtgtgcca tggccaagga agaaagcgcg gtcggcgcaa ggccgatctg gaccgagcgt
     3361 ttcgccagca cagtgtcgtc ggccgcgttc gggaaccgcg ccagcaggtg catgccgcct
     3421 ggcgccagtt ccaccgtcac ccgatcctcg aacgtcgctt tcagtgctgc cgtcaggcac
     3481 cgccggcgct cggcgtaaag cagccgcata cgcctgatat ggcgggcgaa atggccgttc
     3541 cgcatgaaag ccgcgacgac tttctgctcc aggactggct gaccggaggt caacgacctg
     3601 gatgcccgct gaaatgcctt ggccagttca ggcgggacga ccagatagcc tagccgcaat
     3661 gccggaaaca gaaccttgct gaagctgccc gcgtaaagga cccgctgtcc tcgatccagg
     3721 cttttcaacg acggcagcgg ccggccgaca tagcgaaatt cgctgtcata gtcgtcctcg
     3781 acgatccagg attcggcctc ggacgcccat gccaggagtt ccattcgccg cggcagcgat
     3841 agcgaaaccc cagtggggca ttgatgggtc ggcgtcacga ccgcgagcct cgctttcggc
     3901 gcggctttga tgcctgccgc gacgcgcagc ccctcgccat ccactcgcaa gggaacgacg
     3961 cgtgctcccg cggtttcaag cgccagacgg gtcatatgat aaccgggatc ctccatccag
     4021 acaggatcgc cgggccgaag cagaacgttt cgtatcaggg caagcgcacc ctggtagccg
     4081 ccggtgatca acacctgctc cggcgagcat ctgacgccgc gggcgatgcc caaataggcc
     4141 gcgacctcgc tgcgcaacgc aggcgtgccg gaaggatcag cctccgcgag gtccgcgggc
     4201 tgcagcgaac gagctgcctg aaccgtcaaa ttggaccatc gctttcgggg aaatgcgtcg
     4261 agggagggca ggcccatttg gaacggcctt gcgcccgccg cttcggtagg cggagagcct
     4321 ggaaacaaaa gaggcgactg ctccgccact tcgatccgtc gtccgacagg gccggacaca
     4381 attgtccccg cggagcgtcg cgtttcgatg gcaccctcgc ccgcgagcaa ggcataagcg
     4441 gcatcgactg ttccgcgtgc gatcgccagt tgagcagcca gggcacggga cgatggcagc
     4501 ttagcccccg ggagcaactg accggccgca atcgctgtgc gcactcgcac cgcaatttgc
     4561 aggaacagcg ggtccttctg ctgagtatcg agggtgatgt taagcggcat ggtccagtcg
     4621 attcgtcgct ttccggcgct tcacgctatg ccaccaaaga catcagccct gcgagcccga
     4681 aacgaagaaa ccgatcgaac tgtgcgcttt ccagatcaac ggctctccag caggtgcgaa
     4741 ggaccgcgat ggcgaccgac catggatcgt gtgatggccg cgccacgcac ggtccccaca
     4801 ctcggatcta cccaacccgg ttttccacca actccgtcac gacgcccgga tctgccagcg
     4861 tggagatatc gcccaggcta tccgtctcgt ttgcggcgat tttacgtaga atccgccgca
     4921 tgatcttgcc gctgcgagtt ttgggcaggc ccggcgccca ctggatcgca tccggtgacg
     4981 cgattggccc gatctctttg cggacccagg cgataagctc cttgcgcagg gcctcggtgg
     5041 gctcctcgtc ggcgttcaga gtcacatagg cgtagatgcc ctgccccttg atgtcgtgtg
     5101 gaaagcccac gaccgcggct tcggcgacct tgggatgggc gaccagcgcg ctttccactt
     5161 ccgccgtgcc catccgatgg ccggaaacgt tgatcacatc gtcaacccgg ccggtaatcc
     5221 aataatatcc gtcggcatcc cggcgggccc cgtcgccggt aaaatacagt cccttgaagg
     5281 tcgagaaata ggtctggacg aaccgttcgt ggtcgccgaa caccgtgcgc atcatgccgg
     5341 gccatgattc cctgatgcac aaattgccct cggtcgcgcc gtccagttcg ttgccatcgc
     5401 catcgaccag caacggctgg acaccgggca gcggcaacgt cgcggagcct ggtttcaagg
     5461 ccgtggcacc tggcagcggg ctgatcagga tgccgccggt ttccgtctgc caccaggtat
     5521 cgacgatcgg gcagcgcttc tcccccacca cgttgtaata ccatagccag gcttctggat
     5581 tgatcggctc gccaacgctg cccagcaggc gaagcgacgc tcgggacgtc tcctttacgg
     5641 gcccgtcgcc gttgcgcatc aacgcccgga tcgcggttgg cgcggtgtag aaaatgctga
     5701 ccttgtgctt gtcgatcact tgccagaatc ggctgttgtc cgggtagttc ggaatcccct
     5761 cgaacatcag cgtggtggcg ccgttggcga gcggtccgta aagaatatag gtgtgacccg
     5821 tgacccagcc gacgtcggcg gggcaccaat agacctcgcc cgggcgataa tcgaagactt
     5881 tttcgtgggt gtagctggcc cagaccagat agccgccggt agtgtgcagc acgcccttgg
     5941 gcttgcccgt gctgcccgac gtgtaaagaa tgaacagcgg gtcctcggcc gacatcggct
     6001 ccggcggaca gtcaggcgat gcggccgcca ggagcgcgcc ataatcgagg tcgcggcccg
     6061 gttcatgatg caccgaggcc ccggtgacct tgaccacgat gacattcttc acgctcgggc
     6121 agtctttcaa cgcagcgtcg gcattggcct tgagtgcgac ggagcggcca ccgcggcgac
     6181 cttcgtcggc tgtgatcagc aacgtcgaat cgcagtcctg aatgcggttg gccaggctgt
     6241 ctggggagaa cccgccgaaa
//
LOCUS       DASWQN010000291         1770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1217269, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000291 DASWQN010000000
VERSION     DASWQN010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217269"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VGC82_04825"
     CDS             <1..925
                     /locus_tag="VGC82_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422629.1"
                     /translation="RVANDIMVVNRERARGEKPADDFVGLRIFDVSRPSSPRDICHWA
                     CVGMGVHRFTFDGRYAYISAELEGYVGTIVMILDLKDPTRPEEVGRWWMEGQWIAGGE
                     TPSWKGRNHRCHHPIRRGDRLYVSYWYGGGVILDISDMTRPRMISSLDWSPPFGWPTH
                     SLVPIDVPIAGHRWMLVADEHVQPLDPDLSPELPAMVWMVNITDETRPIPVSCFQLPE
                     LVGIKTPLMTACHQPVETIVGNEVPAAWFASGLRVIDISNPLSMKQAAWWMPDVPAGA
                     QRVCSNDVYVDHRGLIYLIDRNRGLSILERV"
     gene            993..1430
                     /locus_tag="VGC82_04830"
     CDS             993..1430
                     /locus_tag="VGC82_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY0422630.1"
                     /translation="MATIPKTLHSHINTAFPADVCLIGTALPNGYAQITSRGSTLVFD
                     DGHFSLWERGKGSTTENLADGSKVTVYFRKPQLRADGILPKGGIARFYGTARLFKSGP
                     IYEEVWTRLIQPEKDRDPDKKGFAVLIEVERAEELDGTPLRLD"
BASE COUNT          353 a          552 c          554 g          311 t
ORIGIN      
        1 gcgagtcgcg aacgacatca tggtggtcaa ccgcgagcgt gcgcgtggcg aaaaaccggc
       61 ggacgatttc gtcggcctgc ggatcttcga tgtcagccgc cccagcagtc cgcgcgacat
      121 ttgccactgg gcgtgcgtcg gcatgggcgt gcaccgcttc accttcgacg gccgctacgc
      181 ctacatctcg gccgaactgg aaggctatgt cggcacgatc gtcatgatcc tggatctgaa
      241 ggacccaacc aggcccgagg aagtcggccg ctggtggatg gaaggacaat ggatcgccgg
      301 cggcgagacg cccagttgga aagggcgcaa ccaccggtgc caccatccga tccgacgcgg
      361 cgaccggctg tatgtcagct actggtacgg cggcggcgtc atccttgaca tcagcgacat
      421 gaccaggccg cggatgatct ccagcctcga ctggagcccg ccgtttggct ggccgacgca
      481 cagcctggta ccgatcgatg tgccgatcgc cgggcatcgc tggatgctgg tcgccgatga
      541 gcatgtgcag ccgctcgacc ccgatttgtc gcccgaattg ccggccatgg tctggatggt
      601 gaacatcacc gacgagacgc ggccgatccc ggtgagctgc tttcagttgc cggaactggt
      661 cgggatcaag acgccgctga tgacggcatg ccatcagccg gtcgaaacga tcgtcggcaa
      721 cgaggtgccg gccgcatggt tcgccagcgg gctgcgggtg atcgatatct ccaacccgct
      781 gtcgatgaag caggccgcat ggtggatgcc ggacgtgccg gccggggcgc agcgtgtctg
      841 cagcaacgac gtttatgtcg atcatcgtgg cctgatttac ctgatcgacc gcaaccgggg
      901 tttgtcgatc ctcgagcggg tatagatccg gagaagccag gcctccgcca gcctcttttc
      961 tgaacagacg acagaacagc cggagcaaga tcatggcgac aattcccaaa acccttcaca
     1021 gccacatcaa caccgcattc cccgccgatg tgtgcctgat tggaactgca ttgccgaacg
     1081 gctatgcgca gatcacctcg cgcggcagca cgctggtgtt cgacgacgga catttctccc
     1141 tatgggagcg agggaaggga tcgacaacgg agaacctcgc cgacggctcg aaggtaacgg
     1201 tctatttccg caagccccag cttcgcgccg acggcatcct gccaaagggc ggcatcgcgc
     1261 gcttctacgg cactgctcgg ctattcaaat cgggaccgat ttacgaggaa gtttggacac
     1321 ggcttattca gccggaaaaa gacagagacc ccgataagaa gggcttcgcg gtgctgatcg
     1381 aggtggagcg ggcggaagaa ctcgacggca cgccactccg actggattga gcgtaagggt
     1441 ctggagacgg gtaaactgct tcgggccagc taatctcgtc ccgcaacgtg atggcatggt
     1501 tcgcctcatg ttcgtcggat gagtcgccac cgcacggtcg ttgccaatct tgtcacggcc
     1561 gcgccaggca cggccatgac acgataatca agcagcccgt cacccgatcc tggacgatac
     1621 cgctggaaac ccattgagcg ctcgtgacgt cttcgcctcc ataacggaat ggaggccgca
     1681 cccgaggcgg tcattcggac cggacctaac cggcgccttc gaccatcgcg cgcgcggatt
     1741 gcacgacatc cagggccgcg tcctcggtcg
//
LOCUS       DASWQN010000292         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2141016, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000292 DASWQN010000000
VERSION     DASWQN010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141016"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VGC82_04835"
     CDS             complement(<1..264)
                     /locus_tag="VGC82_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY0422631.1"
                     /translation="MSDVVVVESPAKAKTINRYLGDGYTVLASFGHVRDLPPKDGSVR
                     PDQDFAMDWESDARGDKQVGAIAKALKGAKTLYLATDPDREGEA"
     gene            complement(281..1406)
                     /gene="dprA"
                     /locus_tag="VGC82_04840"
                     /pseudo
     CDS             complement(281..1406)
                     /gene="dprA"
                     /locus_tag="VGC82_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
     gene            complement(1456..2055)
                     /gene="plsY"
                     /locus_tag="VGC82_04845"
     CDS             complement(1456..2055)
                     /gene="plsY"
                     /locus_tag="VGC82_04845"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY0422632.1"
                     /translation="MAAVFVCLLGYLFGSIPFGLLLTKIAGLGDIRGIGSGNIGATNV
                     LRTGRKGLAAATLLLDGVKGAVAILAARALVYDQDTVLLAGLAAVLGHLFPIWLNFKG
                     GKGVATGLGVLIAASWPVGLAACAIWLLVAATARLSSLASLAAFASAPVSALILEEFG
                     LVKLAFTIAVLVFVRHQGNIRRLLAGTEPRIGHGKTESA"
BASE COUNT          403 a          697 c          698 g          333 t
ORIGIN      
        1 cgcttcaccc tcacgatccg gatcggtggc aagataaagc gtcttggcgc ccttcagcgc
       61 tttcgcgatg gcaccgactt gcttgtcgcc gcgcgcgtcg gattcccagt ccatggcgaa
      121 atcctggtcc gggcgcacgc tgccatcctt tggcggcagg tcacgtacat gcccaaaact
      181 ggccagcacc gtatacccat cgccgagata ccggttgatg gttttggctt tggcggggga
      241 ttcgacaacg acgacgtctg acattgtgcg gtgcggtatc ctaagcaaag gcctccggca
      301 aaagtgccac acggccgccg ggtaacgtct ctatacgccc cgccagctcc aattccagaa
      361 gaaccgacat cgtgacagat gccgacaagt ggcagcgccg caccagatcg tcaaccatag
      421 tcggttccgg ggacagcagc gaaagcaccg ccggttcggc ttcgggcgaa attttggccg
      481 ccatcgggtc gtcatcgaag ggcagccccg gttcgctgaa accagccgta cggggacgcg
      541 cgggggacgg ttccgcaggc agattatcga gcacgtcggc ggcgttttca accaggatcg
      601 cccccctgcc ggatcaggtc gttgcctccc ctcgcccgcg gatcgagtgg cgaaccaggg
      661 acagcgaaga cctcacgacc ggcctcctgg gcgaggcggg cggtgatcag gctgccagag
      721 cgcagcgccg cctcgacgac gacgacgcca agcgacagac ccgcgatgat gcggttgcgg
      781 cgaggaaaat gcctggcctg cggaacagta ccaaccggtg attccgtcac aagaaggtgg
      841 ccgtcggcta tgcgctgatg caatgggccg ttttccgccg gatacggttg gtcgatgccg
      901 ccggcgatca ctgcgatggt tcggccggtg cgcatcgccc catggtgcgc tgcggtatcg
      961 atgccgcgcg ccaggccgga tatgacgacg accgacgccg ccagttccgc cgccaggttt
     1021 tccgccatat gctggccgtt ggccgatgca ttgcgcccac ccaccgccgc gacgcagcgg
     1081 agctgcgcca atccggcctc cccgctcaga atcaggcagg gcggcgcgtc gtccatcatg
     1141 gccagcagcg gcggatactc cggatcgccg agaaagatca tccggccgcc cagggcacgg
     1201 gtgcgctcca gctcctgctc ggcatccgcg atcgacagca cccttggcac cacggttttg
     1261 ccggcggtgc gggccagccg aggcagcaca tcgagcgcgg ccgcggcggt gtggtatcgt
     1321 tggataagcc ggcgataggt cacaggaccg acgccttcgg tgcggatcag gcgcaaatgg
     1381 tcgatccggt cggaccaggt tgacatgccg gtggcgtccc ttcgttgccg ccggcaggag
     1441 tgcgggcggg cccgatcagg cgctttccgt cttgccgtgg ccgatccgtg gttcggtacc
     1501 ggcgagcagg cgacggatat tgccctggtg gcggacgaac accagcacag caatggtaaa
     1561 ggccagctta acgagaccga attcttccaa aatcagcgcc gaaaccggtg ccgaggcgaa
     1621 ggccgccagc gacgccagcg acgaaagccg cgccgtcgcc gcgaccagaa gccagatcgc
     1681 acacgcggca aggccgaccg gccatgaggc ggcgatcagc acgccaaggc cggtggcaac
     1741 gcccttgccg cctttgaagt tcaaccagat cggaaacaga tgaccgagaa ccgcggcaag
     1801 gccagcaagc aggacagtgt cctggtcgta caccagggcg cgggcagcca ggatggccac
     1861 ggcacccttt acgccatcca gcagcaaggt agcggcggcg aggcctttgc ggccggtccg
     1921 cagcacgttg gtcgccccga tgttgccgga gccaattccc cttatgtcgc caaggcccgc
     1981 gattttcgtt agcagcaggc cgaacgggat agaaccaaac aggtagccga gcaggcagac
     2041 gaagaccgcc gccatgtcgt cggtgatcac gtcggcccat ccgctaaggc aggttcgccc
     2101 gttggggcag gttcagccgc tggggcaggt t
//
LOCUS       DASWQN010000293         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1349044, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000293 DASWQN010000000
VERSION     DASWQN010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349044"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1099)
                     /locus_tag="VGC82_04850"
     CDS             complement(98..1099)
                     /locus_tag="VGC82_04850"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEY0422633.1"
                     /translation="MDGIIRAADEHPIRFDLLTENPVYKPFRYPWAYEAWLTQQRVHW
                     LPEEVPLADDVKDWHRNLTDGERNLLTQIFRFFTQADVEVNNCYMKHYSQVFKPTEVL
                     MMLSAFSNIETVHIAAYSHLLDTIGMPEIEYTAFLKYKEMKDKFDYMHGFSIENRFEI
                     AKTLAAFGAFTEGLQLFASFAILLNFPRFGKMKGMGQIVTWSVRDESLHCASIIKLFR
                     TFIQENPELWTEDLRREIYLTCATIVSHEDAFIDLAFEQGPVEGLTAREVKQYIRFIA
                     DRRLIELGLSELFHVAKNPLPWLDEMLNAVEHANFFENRATEYSKASTAGTWEEAFG"
     gene            1485..>2084
                     /locus_tag="VGC82_04855"
     CDS             1485..>2084
                     /locus_tag="VGC82_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0422634.1"
                     /translation="MKVGQAVAEIMKREGIEILCGYPVNHLLEFAAAADIRPVIVRQE
                     RIGIHMADAISRVTSGKKIGAFCMQHGPGTENAYGGVAQAFSESIPMLVIPQGYPRRI
                     AHIAPNYSAVREMRGISKLAEPVFLPHEVANVMRRAFSAIRNGRSGPAIVEIPTDIWN
                     EEIGEIDYTPVGAPKYGPDPAEIRKAAAAILAAKRPVIYA"
BASE COUNT          414 a          625 c          634 g          411 t
ORIGIN      
        1 tagaacaccg ggcgaatgcc agcgcggcgg cttgccatcg atcaagtcca tcgatcatca
       61 gtggcaatcg atcaaacgag ccgccggcgc aaccgggtta cccgaacgcc tcttcccacg
      121 tgccggccgt ggatgccttg ctgtattccg tggcgcgatt ttcgaagaag ttcgcgtgtt
      181 ccaccgcgtt gagcatttca tcaagccacg gcagcgggtt cttcgcgaca tggaacagct
      241 cgctgaggcc cagctcgatc agccgccggt cggcgatgaa acggatgtac tgcttgacct
      301 cacgcgccgt cagcccttcc acgggaccct gttcgaaggc caggtcgatg aacgcgtcct
      361 cgtgcgatac gatcgtcgcg caggtcaggt agatctcccg ccgcagatcc tcggtccaga
      421 gttcgggatt ttcctggatg aatgttcgaa acagcttgat gatcgaggcg caatgcaggc
      481 tttcgtcgcg caccgaccag gtgacgatct gccccatgcc cttcatcttg ccgaaacggg
      541 gaaaattcag caggatcgcg aaggaggcaa aaagctgcag cccttcggta aaggcgccga
      601 aagccgccag ggtcttggcg atttcaaaac ggttttcaat cgaaaatccg tgcatgtagt
      661 cgaatttgtc cttcatctct ttgtatttga ggaaggcggt gtattcgatt tccggcatgc
      721 cgatggtgtc gagcaggtgg ctgtacgccg cgatgtgcac cgtctcgatg ttggagaacg
      781 cggatagcat catcagcacc tcggtgggct taaacacctg gctgtagtgc ttcatgtaac
      841 aattgttcac ctcgacgtcc gcctgggtga aaaagcggaa gatctgggtc agcagattgc
      901 gctcgccgtc cgtcagattg cgatgccagt ccttcacgtc gtcggccagc ggcacttcct
      961 ccggcagcca gtggacgcgc tgctgggtca gccatgcctc atacgcccat gggtagcgaa
     1021 acggcttgta cacggggttt tcggtcagaa ggtcaaaccg gatcggatgc tcgtcggccg
     1081 cgcggataat cccgtccatg tgtatcctgt ccctatagaa gaaagcctga ccggtggtgg
     1141 cgaatcgcga ttgcagcgac cgatcccatc tattgtggtt gggccggcag atcgacgcaa
     1201 cagatagtgt cctcgcctga gattctcgag attaacatgt tcctcgtagc gggctggcgc
     1261 aaggcggccc acatgcttcg tgcacggctg gcgcgggacg cggggggcca tgacgtggcg
     1321 gcatttcatt gaccggaggt cgttccgtgt ttggcccgga ggcgcttagg gcgttgccag
     1381 acgcccgggc tggtcctacc gttgctagtg gagcggcgga ccaagacgcc cgccgctatc
     1441 gacaaagccg catggcacgg atcgaagctt aaagggatgg acgcatgaaa gttggtcagg
     1501 ctgtcgctga aatcatgaag cgcgaaggga tcgagatcct ttgcggctat cccgtcaacc
     1561 acctgctgga attcgccgcg gcagccgata tccggccggt catcgtacgc caggagcgta
     1621 tcgggatcca tatggccgac gcgatatccc gcgtgacatc cggcaagaag atcggcgcct
     1681 tctgcatgca gcacggaccg gggaccgaaa acgcctatgg cggcgtggcg caggcctttt
     1741 ccgaatccat cccgatgctg gtgatcccgc agggctatcc acggcgcatc gcgcatatcg
     1801 cccccaacta cagtgccgtc cgcgaaatgc ggggcatatc caagctggcc gaaccggtgt
     1861 tcctgccgca cgaggttgcc aacgtcatgc gccgcgcgtt cagcgcgatc cgcaacggcc
     1921 gcagcgggcc ggccatcgtc gaaatcccga ccgacatctg gaacgaggaa atcggcgaga
     1981 tcgactatac gccggtcggt gcaccgaaat acggtcccga tccggccgag atccgcaagg
     2041 ccgccgccgc catcctggcc gccaagcgtc cggtcattta tgcc
//
LOCUS       DASWQN010000294         4250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_30078, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000294 DASWQN010000000
VERSION     DASWQN010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30078"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VGC82_04860"
     CDS             <1..814
                     /locus_tag="VGC82_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0422635.1"
                     /translation="ASGVFNFAQGIMVVFAGLTLVSLHAMGVPAWLALLLTLAVMGLL
                     AVAIERLVLRPLVNRPEIMLFMATFGLTWLLIGSGELIFGGDSKAMISDELYLPRGAI
                     DVHILGGLVSLQKLDIAAALVASAMVVCLALLFQYTRIGRALRAVADSHRAALSVGIS
                     LNQIWVVVWFAAGVVALVTGIMWGAKSEVSFALQIVALKALPVLILGGFTSVPGAIVG
                     GMIIGVTEKLGEFYWGPLVGGGIESWLAYVIALVVLLFRPQGLFGEVIIERI"
     gene            911..2035
                     /locus_tag="VGC82_04865"
     CDS             911..2035
                     /locus_tag="VGC82_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0422636.1"
                     /translation="MAGTGAGHDAEPDPKLMFYREAGQYKTSYAADMAVFPLRQDRIG
                     LGIILAIAFVGIPLLGTGFFLDVVMIPLLVYALAAVGLNLLVGYTGLLSLGTGGFMGV
                     GAFACYKLTTWFPDISLLVWLPVSGLFSAALGALFGLPSLRIKGFYLVVATLASQFFL
                     QWCFARVPWLHNYNISGAIEVPQRSLFGVPITGVHATSVTRYYVVLCLVAVVTLAASN
                     LVHGRVGRAWMAVRDMDIAAELIGIRLMPAKLLAFTVSSYVCGVAGAAMVFLWYGSAE
                     PEVFSIDLSFLILFMVIIGGLGSLIGSFLGAALIHVLPVLLRTVPAALGVPIAAATVE
                     HLTFIIVGILIILFLIAEPLGLARLWQTTKQKLIVWPFPY"
     gene            2113..3426
                     /locus_tag="VGC82_04870"
     CDS             2113..3426
                     /locus_tag="VGC82_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422637.1"
                     /translation="MRWATSLLALIALLIGGPVAQAEDSIYVPLFSYRTGPFASSGIP
                     FANGIRDYLTMLNVRDGGIGGVKLMIEECETGYDTKKGIECYEATKSRNPVVLTPLST
                     GIGLAVLPRLAVDKVPMLTMAYGLTASAVGEFFPWAFNPPDTYWDTAGAMLQYIANTS
                     GGLDKLSGKTIGYIYLDAPYGKEPIPLLEQLAKTYGFTLKLYPVAGTEMQNQSSQWLS
                     VRRDKPDWMMMFGWGALHPTALKEAAKVNFPMERFISNFWVSDHDVRAAGDVAKGFRE
                     VAFHAPGTGFPAIKDIKTLVVDKGVTDTPKDQPGEVLYNHGVYNSMLIAEAIANAQKL
                     SGRKVVTGDDVRRGFEAIDLSTERLKAIGLDGFTDPLKLSCADHNGHGRISVQSWDGT
                     KWVRLADPAPPMTAVTQPLVEAAAKDYVEKNAGWPKRTEACDKAS"
     gene            3426..>4250
                     /locus_tag="VGC82_04875"
     CDS             3426..>4250
                     /locus_tag="VGC82_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0422638.1"
                     /translation="MDRMMMGGGTETPPILDISNIEVVYDRVILVLKGVSLTVPKGGI
                     VALLGANGAGKTTTLKAISSLLRAERGEVTKGTISFNGERIDQLSPSELVRRGCIQVM
                     EGRMCFPHLSIEENLLTGAFTRRDGGAAIRRDMAMIYERFPRLKDRRSAVAGYTSGGE
                     QQMCAIGRAMMARPSMILLDEPSMGLAPLVVEEIFDIVRVLNERDGVSFLLAEQNTNL
                     ALQYASYGYILETGRLVMDGEASALRENADVREFYLGMSAGDRKSFRDVKSYKRRKR"
BASE COUNT          713 a         1294 c         1393 g          850 t
ORIGIN      
        1 ggcatccgga gtgttcaact tcgcccaggg gatcatggtg gtgttcgccg gcctaacgct
       61 ggtcagcctg cacgcgatgg gtgttccggc ctggctggcg ctgttgctca cgctggctgt
      121 catgggtttg ctggcggtcg cgatcgagcg gttggtgttg cgcccgttgg tgaaccggcc
      181 ggagatcatg ctgttcatgg ccacgttcgg cctgacctgg ctgctgatcg gctccggcga
      241 gctgatcttc ggcggcgact cgaaagccat gatcagcgac gagctgtact tgccccgggg
      301 tgccatcgac gtgcacatcc tgggcgggct ggtgtcgctg caaaagctgg acatcgcggc
      361 ggcgctggtc gcatcggcga tggtggtatg cctcgcgctg ctgtttcaat acacgcgcat
      421 cggacgagcg ttgcgcgcgg tggcggacag tcatcgggcc gcgttgtcgg tcggtatttc
      481 gctgaaccag atctgggtcg tggtgtggtt cgccgccggc gtcgttgccc tggtcaccgg
      541 catcatgtgg ggcgctaagt cggaggtatc gttcgcgctc cagatcgtcg cgctgaaagc
      601 gcttccggtg ctgatcctgg gcggcttcac ctcggtcccc ggcgcgatcg tcggtggcat
      661 gatcatcggg gttacggaaa aactcggcga gttctactgg ggcccgctgg tcggcggcgg
      721 catcgaaagc tggctggcct atgtgatcgc cctggtcgtt ctgctgttcc gcccacaggg
      781 gctgttcggc gaagtcatca tcgagcggat ataagatgcc gccttcgccc cgcatagcgg
      841 ttggttatcg tgtcagggcc gggcttggcg cggccacgca cgactttgtc tcaacgcagg
      901 aaaatcgtgg ttggccggca caggggccgg ccatgacgcc gaaccggacc ccaagctgat
      961 gttctaccgc gaggccggcc agtacaagac cagctatgcc gccgacatgg cggtgtttcc
     1021 gctgcgccag gaccgtattg gcctcggcat catcctcgcg atcgcattcg tcggaatccc
     1081 gctgctcggt acagggttct tcctggatgt ggtaatgatc ccgctgctgg tttacgcatt
     1141 ggccgcggtc gggctcaatc tgctggtcgg ctacaccggg ctgctgtcgc tgggcacggg
     1201 cggcttcatg ggcgtgggcg ctttcgcctg ttataaactg accacctggt ttcccgacat
     1261 cagcctgctg gtctggctgc cggtgtccgg gctgttctcc gccgccctcg gtgctttgtt
     1321 cggcctgccg tcgctgcgta tcaagggctt ctacctggtg gtcgcgacgc tggcctcgca
     1381 gttcttcctg caatggtgct tcgcgcgcgt cccgtggctg cacaactaca acatctccgg
     1441 tgcgatcgag gtgccgcagc gcagcctgtt cggtgtgccg atcaccgggg tgcatgcgac
     1501 ctcggtcacc cgttactacg tcgtgctgtg tctggtcgca gtggtgacgc tcgcggcgtc
     1561 aaacctcgtg catggccgcg tcgggcgggc gtggatggcg gtgcgggaca tggacatcgc
     1621 tgcggaactg atcggcatca ggctgatgcc agcgaaattg ctcgccttta ctgtctcctc
     1681 ctatgtttgt ggcgtcgccg gggcggcgat ggtcttcctg tggtatggca gcgccgaacc
     1741 cgaggtgttc tcgatcgacc tctcgttcct gatcctgttc atggtcatca tcggcggcct
     1801 tggttccttg atcggcagct tcctgggcgc ggcgctgatc cacgttctgc cggtgctgtt
     1861 gcgcaccgtt cccgccgccc tgggcgtgcc gatcgccgcc gcgactgtcg agcacttgac
     1921 gtttattatc gtcggcattc tgattatcct gtttctcatc gccgaaccgc tggggcttgc
     1981 gcgcctgtgg cagaccacaa agcagaaact tatcgtatgg ccgtttccat actgagaacg
     2041 agaccaacaa gactgggaaa aatgccaaca aaacgaaaca gccccgcatc gggcttaaat
     2101 agggagagcg tcatgaggtg ggccacatcg ctgctcgcgc tgatcgcgtt actgatcggc
     2161 ggaccggtgg cacaggcgga ggacagcatc tacgtcccgc tgttcagtta tcgcaccgga
     2221 ccgttcgcgt cgtcgggcat cccgttcgcc aacggcatcc gcgactacct gacgatgctg
     2281 aacgtgcgcg acggcggtat tggcggcgtg aaactcatga tcgaggagtg cgaaacgggc
     2341 tacgacacca agaagggcat cgaatgctac gaggccacga agtcacgcaa tccggtcgtc
     2401 ctgacgccgc tgtccaccgg gatcgggctt gccgtcctgc ctcgtctggc ggtggacaag
     2461 gtgccgatgc tgacgatggc gtacggcctg acggcctcgg ctgtcggcga gttctttccg
     2521 tgggcgttca acccccccga cacgtactgg gataccgccg gcgcgatgct gcaatacatc
     2581 gccaacacct cgggcggact ggacaagctg tccggcaaga ccatcggcta catctatctc
     2641 gacgcgccct atggcaaaga accgatcccg ctgctggagc agctcgccaa gacctacggc
     2701 ttcacgctga aactgtaccc ggtggccggg accgagatgc agaaccagtc ttcgcaatgg
     2761 ctcagcgttc gccgcgacaa gccggactgg atgatgatgt tcggttgggg cgctctgcac
     2821 cccaccgcgt tgaaggaggc cgccaaggtc aacttcccga tggagcggtt catctccaac
     2881 ttctgggtca gcgatcatga tgtgcgtgcc gccggcgatg tggcgaaagg cttccgcgaa
     2941 gtggctttcc atgcccccgg caccggcttc ccggcgatca aggacatcaa gaccctggtt
     3001 gtggacaagg gcgtcaccga cacgccgaag gaccagcccg gcgaggtgct gtacaaccac
     3061 ggcgtttata attccatgct gatcgccgag gccatcgcca acgcgcagaa gctcagcggc
     3121 cgcaaggtgg tgaccggcga tgacgtgcgc cgtggcttcg aggcgatcga cctgtccacc
     3181 gaacggctga aggcgatcgg cctggatggc ttcaccgatc cgctgaaact aagctgcgcc
     3241 gaccataacg gccatggccg gatctccgtg cagtcctggg acgggaccaa gtgggtgcgc
     3301 ctggccgacc cggcgccacc gatgaccgcg gtgactcagc cattggtgga ggcagccgcc
     3361 aaggactacg tcgagaagaa cgccggctgg ccgaaacgta ccgaggcttg tgacaaggcg
     3421 tcctgatgga tcggatgatg atgggcggag ggactgaaac cccgcccatc ctggatatca
     3481 gcaacatcga ggtcgtttac gaccgggtga tcctggtgct gaaaggcgtc tcgctgactg
     3541 tgcccaaagg cggcatcgtg gccttgctgg gggcgaacgg ggcgggcaag accaccacct
     3601 tgaaggcgat ctccagcctg ttgcgggcgg aacgggggga ggtcaccaag ggcacgatca
     3661 gcttcaacgg cgaacgcatc gaccaactgt cgcccagcga gctggtgcgc cgcgggtgca
     3721 tccaggtgat ggaaggccgc atgtgctttc cgcatctgtc gatcgaggaa aacctgctga
     3781 ccggcgcctt cacccgccgt gacggcggtg cggccatccg ccgcgacatg gcgatgatct
     3841 atgagcgttt tccgcgcctg aaggaccgcc ggagcgcggt tgcgggctac acctcgggcg
     3901 gcgagcagca gatgtgcgcg atcggccggg caatgatggc acggccttcg atgatcctgc
     3961 tggacgagcc gtcgatgggt ttggcgccgc tggtcgttga agaaatcttc gacatcgtcc
     4021 gggtactgaa tgagcgtgac ggcgtttcat tcctgctcgc cgaacaaaac accaacctcg
     4081 cgctgcaata tgccagctat ggctacatcc tggaaaccgg ccgcctggtg atggatggcg
     4141 aggcgtccgc cttgcgcgag aacgccgacg tccgggagtt ctacctcggt atgtcggccg
     4201 gcgatcgcaa atcgttccgc gatgtcaaga gctacaagcg gcggaaacgc
//
LOCUS       DASWQN010000295         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1084966, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000295 DASWQN010000000
VERSION     DASWQN010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084966"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(389..613)
                     /locus_tag="VGC82_04880"
     CDS             complement(389..613)
                     /locus_tag="VGC82_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422639.1"
                     /translation="MAEYKFAVGDRVALMANSSNINVRPGIYTIVRRMPASSQGCQYR
                     AKSALDNHERVVDEAQLRRALSPAVDAGLA"
     gene            complement(667..873)
                     /locus_tag="VGC82_04885"
     CDS             complement(667..873)
                     /locus_tag="VGC82_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010501677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY0422640.1"
                     /translation="MASGTVKWFNPQKGFGFIQPADGSKDVFVHISAVERAGLKTLNE
                     GQTISFELERGQQGKTSAVNLQAS"
     gene            complement(1064..>1985)
                     /locus_tag="VGC82_04890"
     CDS             complement(1064..>1985)
                     /locus_tag="VGC82_04890"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY0422641.1"
                     /translation="LSLPELTRPDRKQLVGLLPDDPAMVLEEGAQVTAASAEGTGIGG
                     NSIGHVTSSYHSPTLGRGFALALVEGGRALIGSQVHVPTTGGAILVAVVDPVFVDKAG
                     ERSRPRPVPRRGIEPLLAAVPQPALVARASSSVRLAVLAATTRLSVRAGSAASTAIGL
                     ALGVLLPTAPCRSVITRDRVALWLGPDEWLIVAPENASTLAARAAKAAGDHPVSVVDV
                     SHRARTLEITGRHAAWCLNAFCALDLDVHAFPVGMCTRTLLGRAEVVLWRIAPEVFHL
                     DVPRSFVQYVWACLEEARLEFADADVVSAS"
BASE COUNT          401 a          653 c          600 g          331 t
ORIGIN      
        1 tcgggtaagc gaattgaagc ccgccaacag gtcaagagtt ggtccatcct gatccgtcag
       61 cgtccgtccg caactaagcg cgtcaggtcg agcgggccag gagatccgtc ttcgtgggca
      121 aagtccccag gggctggaca cagcgctcca gcggtgcccg agtctggggt ggcggttggc
      181 cataatgcgt catggccgtg cttggcacgg ccacccacga ctttgtctca acaagtgaaa
      241 gtcgtgcatg gccagcacta ggctagccga ggcgcaagcg ggagagaccg cgcaacggca
      301 gctcatccgg cgcaaattaa acgaagcacg taatggcatg ctaggccggc atcacccggc
      361 gtgaagccgt catttgccag ccgaagcctc aagccaatcc tgcatccacc gcaggcgata
      421 aggcacgccg aagctgcgcc tcgtccacga cacgttcgtg gttgtcgagt gcgctctttg
      481 cccgatactg gcacccctgg ctggacgccg gcattcttcg gacgatggta tagattccgg
      541 ggcgaacatt gatattggaa ctgttcgcca tcagcgcgac cctgtcgccg accgcgaact
      601 tgtactcggc caatgggtat ccctccggca gcatcaaggg cggcgcacag ggcgccgccc
      661 ggcatgtcag ctcgcttgaa gattgacagc cgaggtttta ccctgctgcc cacgctccaa
      721 ctcgaagcta atcgtctgac cctcgttgag ggtctttagc cctgctcgtt caacggccga
      781 gatatggacg aaaacgtcct tagagccgtc ggccggctga atgaaaccaa agcccttttg
      841 tgggttgaac cacttaacag tacctgaagc cacgtgaagt ctccgcaact ggctgaaacc
      901 cacgcacatg tgggttatcc gacttttcaa tgggggagag aacggcgcgc cgcggtgcgg
      961 tttgatgacg caaagcggcg cgaattaggc atcttgcccc ggctgaaaag ttcagccgtc
     1021 caatacccat atagtaccca gggaaaagaa gacaacgatt atttcagctg gctgacacaa
     1081 catcggcatc ggcgaattcc agccgcgcct cctccaggca ggcccagaca tactgaacga
     1141 aggaacgcgg cacatcgagg tggaaaacct ccggcgcgat ccgccacagc accacctcgg
     1201 cccgcccgag caacgttcgc gtacacattc cgaccggaaa cgcgtgcaca tccaggtcga
     1261 gggcacagaa cgcgttcagg caccaggcgg catgccggcc ggtgatctcc agggttcgcg
     1321 cccgatgaga cacatcgacg acactcaccg gatgatcgcc cgcggccttc gccgcccggg
     1381 ctgccagggt cgacgcgttt tccggcgcca cgatcagcca ttcgtccggt cccagccaca
     1441 acgcgacgcg gtcgcgcgtt atgacggatc ggcatggcgc cgtcggaagc agcaccccga
     1501 gcgccagtcc gatcgcggtg ctggcggccg aacccgcccg caccgacagg cgggtggtcg
     1561 ctgcaagaac cgccaatcgc acggagctgc tggcgcgcgc gaccagtgcc ggctgcggga
     1621 ccgccgccag cagcggttcg atgccgcgtc gaggcaccgg tcgcggacgc gatctttcgc
     1681 ctgctttgtc cacgaatacc ggatcaacga ccgccacaag gatcgcgccg ccggtcgtcg
     1741 ggacatggac ctgtgagccg atcagcgctc gtccgccttc taccagtgca agcgcgaagc
     1801 cgcgacccag cgttggactg tgataggacg atgtaacgtg gccgatactg ttaccgccga
     1861 tgccggtgcc ttccgccgaa gcagccgtga cctgggcgcc ttcctccagc accatcgccg
     1921 ggtcgtccgg tagcagcccg acaagctgct tgcggtccgg ccgcgtcagt tccggcagcg
     1981 acagc
//
LOCUS       DASWQN010000296         3869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_161897, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000296 DASWQN010000000
VERSION     DASWQN010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161897"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..2094)
                     /locus_tag="VGC82_04895"
     CDS             complement(40..2094)
                     /locus_tag="VGC82_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY0422642.1"
                     /translation="MSYTIGIDVGGTFTDIVVSASGGETIIAKAATTPVDQSKGVLDG
                     IAVAAERLGLTPSALLQRTSRIVHGTTAATNALLEGKTPRVGMLTTEGHRDVIEMREG
                     LKPDRYNLRMTPPIPLVARRLRLPVRERIRADGSVEIALDSGSLEGAIDRLRDAEVQA
                     VAICFLHAWRNPAHEQQAAEAVRNRLPGVYVTTSFEVLPQIKEFERFSTTVANAAVGP
                     VIQNYLGRVQSRLHEAGFDGELLVILSHGGVASVPEAIRLAVGTTLSGPAGGVAAAVA
                     LARGGLASNIIGFDMGGTSTDIAVVRDGQPTLSGDKSVAGARIALPSLDIVTLGAGGG
                     SIGKRDRSGLLAVGPLSAGAVPGPACYGLGGALATVTDANLVLGYLDPANFLGGRRHL
                     DRDAAGAAVAKLAEELGIDTAEAAAGIHRVVNSRMADGVRVATVRRGVDPREYTLLAF
                     GGAAGLHVAAVAAELGIRRVVVPVAASVLSAWGMLNTDLRVEISRSLGQSGSIDIGGL
                     NAAFAAMEVEGRARLAWFDGEVTLRRSADMRYGEQVFEIPVALDDVDWRGDTLTKDLG
                     DRFHAAHERLYTYALRDQEVVLVNARLSVIGRLPQTGQAISSIPLEHAEARAERLVYL
                     SGWTQVPVYDLSSLAMDQSIRGPAIVESDTTTILLRNGDTARFDARGWLDVGIDARAA
                     AA"
     gene            complement(2091..3224)
                     /locus_tag="VGC82_04900"
     CDS             complement(2091..3224)
                     /locus_tag="VGC82_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0422643.1"
                     /translation="MPPAIGRRGFIQSASATGSFLACYGVADRSKAAAPVRITPPVVE
                     KLTIQAVVDTNHDIFISGAQAPGVGVERVRSPAAFDGKTLESQWGLSLHLVSTKGSDT
                     RRYLLDFGFTPDVLANNLSLLKIDPATLDALILSHGHLDHLGGLIGFLQASRPSMRED
                     LRLYVGGEDAFCHRVQQQPNGSFAPYGVLDRRDLKSLRVQPVLSELPIVIEGHAFTTG
                     VVPRTSIETVLPNTFVVYGEKNGVGCDTINYGDHHFTAEELAGTPVPDQHWHEHATCF
                     QVGDRGLVVITSCGHGGILNTVRRAQEITGVEKIHALCGGFHLAPASAAYLDQIMGEL
                     KKLPIDHLIPMHCSGSNFLETARREMPDKVVLCTTGSRFTFTA"
     gene            complement(3230..3691)
                     /locus_tag="VGC82_04905"
     CDS             complement(3230..3691)
                     /locus_tag="VGC82_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0422644.1"
                     /translation="MCETQLKVTSTFATHLLRVENLSSLNQNGERKINKASCVDPGDW
                     RRLAALLRDPDTGETLGGMIGKTSYGLLFIDLVYLPEDVRGQDIGSRLLAMMEQEGIA
                     RGCKSGFLFTVTFQAPAFYARHGWTEFGRIACDPPGTARIFMTKTLSEPQG"
BASE COUNT          661 a         1238 c         1279 g          691 t
ORIGIN      
        1 cggtatcacc ctgcatcgct ttgcctcctg atcacccggt tacgccgccg cggcgcgtgc
       61 gtcaatcccg acgtccagcc agccgcgcgc atcgaatcgg gccgtgtcgc cgttgcgcaa
      121 caggattgtg gtggtatcgg attccacgat cgccggaccg cgaatgcttt gatccatcgc
      181 gagcgacgac agatcgtaca ccggcacctg ggtccagccg gaaaggtaca ccagccgctc
      241 tgctcttgct tcggcatgtt ccagcgggat gctggaaatt gcctggcctg tttgcggcag
      301 ccggccgatc accgacagcc tggcgttcac aagcaccact tcctggtccc tcagggcgta
      361 ggtataaagg cgttcgtggg cggcgtggaa ccggtcgccg agatccttgg tcagagtgtc
      421 gccgcgccaa tcgacgtcgt ccagcgccac cgggatttcg aatacctgtt cgccataccg
      481 catgtcggct gaccttcgca gcgtcacctc gccgtcgaac cacgccaggc gggctcggcc
      541 ctcgacttcc atcgccgcga aggctgcgtt caggccgccg atatcgatgc tgccggattg
      601 ccccaggctg cggcttatct ccacgcgaag atccgtgttc aacatgcccc aggccgacag
      661 gaccgacgcc gcgaccggca ccaccacccg gcggataccc agttcggcgg ccacggcggc
      721 gacgtgcaaa ccggcggcgc cgccgaacgc cagcaaggtg tattcgcgcg gatcgacgcc
      781 gcgtcggacg gtggccaccc gcacgccgtc cgccatccgg ctgttgacga cgcggtggat
      841 gccggcagcc gcctcggcgg tgtcgatgcc cagttcctcg gccagcttgg ccacggcagc
      901 ccccgcggcg tcccggtcca gatggcgccg gccacccagg aagttcgccg gatcgagata
      961 tccgagcacg agattggcgt cggtcacggt cgccagggcg ccgcccaggc cgtagcaggc
     1021 aggcccggga acagcgccgg cactcaatgg tccgaccgcg agcaggccgg agcggtcgcg
     1081 cttgccgatg gagccgccgc cggcaccaag ggtgacgatg tccaggctgg gaagtgcgat
     1141 gcgagccccg gcgaccgatt tatccccgga cagcgtcggt tgcccgtccc gcacgacggc
     1201 gatgtcggtg gacgtgccgc ccatgtcgaa accgatgatg ttcgacgcca ggccgccgcg
     1261 ggcaagtgcc accgcagcgg ccacgccgcc ggccgggccc gacaaagtgg tgccgaccgc
     1321 aagccggatc gcctccggta ccgaggcaac gccgccatgc gacaggatca ccagcagctc
     1381 tccgtcgaac ccggcctcgt gcaggcggga ctggacacgc ccgaggtagt tctgaatgac
     1441 cggcccaacc gcggcattgg cgaccgtggt ggagaaacgc tcgaactcct tgatctgcgg
     1501 caacacctcg aacgaggtcg tgacatagac gccgggcaag cggttgcgca cggcctcggc
     1561 ggcctgctgc tcgtgagccg ggtttcgcca ggcgtgcagg aagcagatgg cgaccgcttg
     1621 cacctcggcg tcgcgcaggc ggtcgatcgc accctcaagc gatccggaat ccagcgcgat
     1681 ctccaccgat ccatccgctc ggattcgctc cctgaccggc aggcgcaggc ggcgcgcgac
     1741 cagcgggatc ggtggcgtca tgcgcaggtt gtagcgatcc ggcttcagcc cctcgcgcat
     1801 ttcgataacg tcgcgatgac cttcagtggt tagcatcccg acgcgggggg tcttgccttc
     1861 cagcagcgca ttggtggcgg ccgtggtgcc gtggacgatc cgggacgtgc gttgcaacag
     1921 tgccgacggg gttagaccca ggcgttcggc agccacggcg attccgtcaa gcacgccttt
     1981 ggattgatcc acgggcgtcg tcgcggcctt ggcgatgatg gtctcgccgc cggatgcgga
     2041 gacgacgatg tcggtgaagg tgccgccgac atcgatgccg atcgtgtagc tcatgcggtg
     2101 aacgtgaaac ggctgccggt ggtgcagagg acgaccttgt ccggcatctc ccgtctggcc
     2161 gtttccagga agttgctgcc gctgcaatgc atcgggatca gatggtcgat cggcaacttt
     2221 ttcagttcgc ccatgatctg atccagatag gccgccgagg ccggtgccag atggaacccg
     2281 ccgcacagcg cgtggatttt ctcgacgccg gtgatttcct gcgcccggcg cacggtgttg
     2341 agaatgccac catgcccgca cgaggtgatc accactagcc cgcggtcgcc gacctgaaag
     2401 caggttgcgt gctcatgcca gtgctgatcg ggcaccggcg tgccggcgag ttcctcggcg
     2461 gtgaaatggt ggtcgccgta gttgatcgta tcgcagccga cgccattctt ttcgccatac
     2521 acgacgaagg tgttgggcag cacggtctcg atgctggtgc gcggcacgac gccggtggtg
     2581 aacgcgtggc cctcgatgac gatcggcagt tcggagagga ccggctgcac ccgcaacgat
     2641 ttcaggtcgc gccggtccag cacgccgtag ggggcaaacg agccgttggg ttgctgctgg
     2701 acgcggtggc agaacgcgtc ctcgccgccg acatatagcc gaaggtcctc acgcatcgag
     2761 ggccggctgg cctgcaggaa gccgatcagg ccaccgaggt ggtcgagatg cccgtggctc
     2821 aggatcagcg catcgagcgt cgccgggtcg attttcagca ggctcagatt gtttgcgagg
     2881 acatccggcg taaagccgaa atccagcaga tagcggcggg tgtcgctgcc tttggtggag
     2941 accaggtgca gcgagaggcc ccactggctt tcgagcgtct tgccgtcgaa cgcggcgggg
     3001 gagcgcacac gctcgacccc gacccccggt gcctgggcgc cagagatgaa gatgtcgtgg
     3061 ttggtatcca cgacggcctg gatggtcagc ttttccacga ccggcggcgt gatccgcacc
     3121 ggtgccgcgg ccttgctgcg atcggcgacg ccgtagcagg cgagaaacga tccggtggcg
     3181 ctggccgact ggatgaaccc gcggcgtccg attgctggcg gcatcgtgtt tatccttgcg
     3241 gttccgatag cgtcttggtc atgaaaatcc gggcggtgcc gggaggatcg caggcgatgc
     3301 ggccgaactc ggtccagccg tggcgggcat agaaggcggg cgcctggaag gtgacggtga
     3361 acaggaagcc tgacttgcag cctcgggcga tgccctcctg ctccatcatc gccagcaggc
     3421 ggctgccaat atcctggccg cgcacgtctt cgggcagata gaccagatcg atgaacagca
     3481 gcccgtacga ggtcttgccg atcatcccgc ccaaggtttc gccggtatca ggatcgcgaa
     3541 gcagggcagc caggcggcgc cagtccccag ggtcaacgca tgaagctttg ttaatctttc
     3601 gctcgccatt ttggtttagt gatgacagat tttctactcg taataggtgt gtggcaaatg
     3661 tggatgtaac tttaagttgg gtttcgcaca cattttgaat ttaaaggcgc tatcacttgg
     3721 cttggaaggt gtgtgccttc cagcaagagc ctgtctttca agcgggctaa tttaccgcac
     3781 ctatttcatg cgtagcccct ggggtctact ggcagccagt gcatgctgcc ctggaggatc
     3841 ggtttaccgt tatcgtcggc aacgcctcg
//
LOCUS       DASWQN010000297         2870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_425725, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000297 DASWQN010000000
VERSION     DASWQN010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425725"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VGC82_04910"
     CDS             <1..365
                     /locus_tag="VGC82_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422645.1"
                     /translation="IRENSLKVQTQTDADGLNAAQIAQIRARLSSMGRDAAKYSDADI
                     ESMHAERARRFVTDAPTSAAEAAKIILDGVKADRLRILVGPDAHIIDRMVREDPDHAY
                     EPEFYERFATEAGWYPGR"
     gene            complement(759..1109)
                     /locus_tag="VGC82_04915"
     CDS             complement(759..1109)
                     /locus_tag="VGC82_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422646.1"
                     /translation="MDENQVQGGSGPALAAHSKDASTPGQTPASSAKGNDGHPAGSAG
                     ETVNRVSEQAREAAARMSASVSDAAGRARQGLSEQGSWAANQGSALVREQPFVALALT
                     GAVCLMIGILLGRR"
     gene            1543..2775
                     /locus_tag="VGC82_04920"
     CDS             1543..2775
                     /locus_tag="VGC82_04920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0422647.1"
                     /translation="MVQDALAEVEVADRLGFDYVWANEHHFLEEYSHNSAPEVFLAAA
                     AGRTRQIHVGHGVVLSPPGYNPPAHVAERIAMLDLVSSGRAEWGTGSSASRAELEGFN
                     VDPAQKKLMWAEATEQSANMMAMSPYPGFKGDFFSMPARNVIPKPLQKPHPPIWLACS
                     NKETIHVAARNGVGALAFAFVDPVEAAKWAGEYYEIIKSDECVPIGHAVNANIAMATG
                     FSVHHDEAEAIRRGGDGFQFFGFALGHHYVYGEHIPGVTNVWERFERGRDAMPPVIGN
                     GIGTPGQLIDRLRQYQDAGVDQVIFVQQAGRNTHDDIIASLELFAAEVMPVMKADQAA
                     RDARKQAELAPYIEAALKRKKWMRPLALDAIPPIPAYGRSVVASDGTPNAISARRGGG
                     INIPSEDPSEKLRAVGDD"
BASE COUNT          586 a          911 c          882 g          491 t
ORIGIN      
        1 gtattcgcga aaactcgctc aaggtgcaaa cccagaccga cgcggatggg ctgaacgccg
       61 cacagatcgc gcagatccgc gcccggctca gctctatggg ccgggacgcg gcgaaatatt
      121 ccgacgccga catcgagagc atgcacgccg agcgcgcccg ccgcttcgtc accgacgcgc
      181 ctaccagcgc ggccgaggcg gcgaagatca tcctggacgg tgtcaaggcc gaccggcttc
      241 gcatcctggt cggacccgac gcccacatca tcgatcgcat ggtgcgcgag gatcccgacc
      301 acgcctatga accggagttc tacgagcgtt tcgctaccga agcaggctgg tatcccggcc
      361 gctgacgacg gagccaggga tcgaagtaat cggagaggcc cgctatccgc ggaaaccgca
      421 ctcaacacgg tcagcgcgca ccgagggaac cagtgccgtc ctcggtgcgc gggaccggga
      481 aggccactgg tcgaaaacag tccgctggca gtgggaagtc aggcgcgcgc aaccttggca
      541 acgccaacga aagggtcctc cgagccggcg cacctggagg cgacgcacgt ctgtgcattc
      601 cttttggcag caaggaggtc gcgctccggc ttggagcccg ttaccgcgcc ggcggctagt
      661 acgcgccgcc aggcatcgat ctcgaccggc ttcaggcaac gtgggtggct ataaccgcat
      721 tgccgcaacg ggcaggttgg cagtcgcagg tacggccgtc agcgccggcc gagcaggatg
      781 ccgatcatca gacagactgc cccggtcagc gccagggcga caaagggttg ctcgcgcacc
      841 aatgccgaac cttgattagc tgcccaactc ccctgttcag aaagaccttg gcgtgcccgt
      901 cctgccgcat cggaaaccga tgcgctcatc cgggcagcag cttcgcgggc ttgttcagaa
      961 accctgttga ctgtctctcc ggcactgcct gctgggtgtc cgtcgttccc cttcgcactt
     1021 gatgccggtg tctgacccgg agtggaagca tccttgctgt gagctgccaa agccggccct
     1081 gagccgcctt gaacctgatt ctcatccata gcgctctccc atttaaaaga tctgccgcga
     1141 tgtgcggcat agtacaagcg gatccagcat cgcaactcgc caggacactt cgtccaggca
     1201 tctggatcca gcacctttgt tgaatatgga acgtcggctg gatttacgcc ggttaccata
     1261 gcagcagcca cggcagggaa cgatcatcaa atggagatcg cacagcacca ccgattcagc
     1321 cagattggat tctcaatgcg agcgggtcga gggcgaagga ttcgaagtca cgtgtatccg
     1381 tcgcctgaca ggcagacagg aggggcgcta gacgtcccga ccagccgccg ctactgagca
     1441 gtagaataca ccgacgaggc gaaggagacg taagccatga ggttcggcct gatctacgaa
     1501 ctgcaactgc cacgtccgtg ggacgaggaa tccgagcacc ggatggtcca ggacgcgctg
     1561 gccgaggtgg aggtcgcaga ccggcttggc ttcgattatg tctgggccaa cgaacaccac
     1621 ttcctggagg agtattccca caactccgcg cccgaagtat tcctcgctgc cgccgctggg
     1681 cgcacaaggc aaatccatgt cggccacgga gtcgtgctct cgccgcctgg ctataacccg
     1741 cccgcgcatg tggcggaacg gattgccatg ctcgacctcg tctcctccgg tcgcgctgaa
     1801 tggggcaccg gatcctcggc gtcccgcgct gaactagaag gtttcaacgt cgatccggcg
     1861 cagaaaaagc tgatgtgggc tgaagcgacg gagcagagcg ccaacatgat ggcgatgtcg
     1921 ccctatcccg gcttcaaggg cgacttcttc tcgatgcctg cccgcaacgt cattccgaag
     1981 ccgttgcaga agccgcaccc gccgatctgg ctcgcctgct cgaacaagga gacaatccac
     2041 gtcgccgcgc gcaacggcgt cggcgcgctc gccttcgctt tcgtcgatcc cgttgaggcc
     2101 gcgaagtggg ccggcgaata ctacgagatc atcaagtccg atgaatgcgt gccgatcggc
     2161 cacgcggtga acgccaacat cgcgatggcg acgggcttct ctgtccacca tgatgaggca
     2221 gaggcgatcc ggcgcggcgg cgacggcttc cagttcttcg gcttcgcgct tggccaccac
     2281 tacgtttatg gcgagcacat cccaggcgtg acgaatgtgt gggagcggtt cgaacgtgga
     2341 cgcgacgcta tgccgcccgt gatcggcaac ggcatcggca cgccggggca gctcattgac
     2401 cgactgagac agtatcagga cgcgggcgtc gatcaggtga ttttcgtcca gcaggcgggc
     2461 cgcaacacgc acgacgacat catcgcatca ctcgagttgt ttgccgccga ggtcatgccg
     2521 gtgatgaaag ccgatcaagc ggcacgcgac gcgcgtaagc aggccgagct ggcgccgtat
     2581 atcgaagcgg cgctcaagcg gaagaagtgg atgcgcccgc tcgcgctaga tgcaattccg
     2641 cctatccctg cttacggccg ttctgtcgtt gcaagtgacg gcactccgaa cgccatctct
     2701 gccaggcgcg gtggcggcat caacatcccg agcgaggatc catccgaaaa attgcgtgcg
     2761 gtcggcgacg actaggattc aggtagtcaa cggactcgaa gaatggatcg cagcaaaatt
     2821 agggtatgaa ccctcaccac ctatcagttg ctggaacgcc ccaaagttgt
//
LOCUS       DASWQN010000298         3806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1085196, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000298 DASWQN010000000
VERSION     DASWQN010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085196"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1347
                     /locus_tag="VGC82_04925"
     CDS             310..1347
                     /locus_tag="VGC82_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0422648.1"
                     /translation="MRILPLLLVALSVMIAPAAIAQTPRIVMEEMMVPSSDAGIEIFV
                     RNKRPADMISFRPERTLLYVHGATYPSSTAFDLQLGGMSWMDYIAERGYDVYLLDLRG
                     YGKSTRPKEMNDKPEANAPIVRGDTAVKDISTVVDFILKRRNIPRLDLLGWSWGTTLM
                     ATYTTQNAAKVERLVLYAPAWIRTTPSLVQAGPGPIPAYRAVRKDQALGRWLTGVAEN
                     KKASLIPAGWFDAWADATWATDPEGGKMDPPVLRAPNGVVQDGAEFFGASKAYYDPAK
                     VTVPTLLIHAEWDQDTPSYMAQTLFPLLVNSPGKRYVQLAEGTHTIIMEKNRLELFKA
                     VQAFLDEAAGL"
     gene            complement(1355..2440)
                     /locus_tag="VGC82_04930"
     CDS             complement(1355..2440)
                     /locus_tag="VGC82_04930"
                     /inference="COORDINATES: protein motif:HMM:NF033816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cj0069 family protein"
                     /protein_id="HEY0422649.1"
                     /translation="MDTERHLPLGHTVAILSRGDAAARRDTTPQNSRFVRVFEALAAA
                     GIEARPAIYDETFAGAVRDQLLAVDGVLVWVDPIHQGKTRAALDPLLREISMCGPWVS
                     AHPDVILKMGVKEVLYRTRHLGWGTDTHRYDSAAAFRDEFPLRLRSSGPRVLKQNRGN
                     GGQGVWKVESLPNTDATVTVLHAQRGSRPEQMPLDDFIGRCEPYFGWGGCIIDQAFQP
                     RLPEGMIRCYMSGPKVAGFGHQLIKALIAPPPAGPDSPEAQPGPRIMHGPEAPPFQAL
                     RRLMEEEWTPQMMQTLDIDQTSLPVIWDADFLFGRRDAAGADTYVLCEINASSCFAIP
                     DEAPAAIARTVKDRLAQVRREDSGRPT"
     gene            2670..3425
                     /locus_tag="VGC82_04935"
     CDS             2670..3425
                     /locus_tag="VGC82_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0422650.1"
                     /translation="MDGLSWAFFALAAFAGGFVSGFSGFAMGLVVSGAWLHIITPLQT
                     AALIAGYGLLTQGYGIIKLRHAFNWRTILPLALGSAVGIPVGVYLLTYISPLVLRAGV
                     GAVLMTYSAYSLLRPAIGPFAIGTGGDLAIGFANGILGGMTGLGGVISTISCQWRGWP
                     KDVQRGVFQPVLFASFVVIDVSLGFAGAFAPETVGLYAVGLPFLLAGLWGGFKLYGSI
                     DDETFRKSVLLLLLISGLSLILSAGGLPGASGS"
     gene            3571..3708
                     /locus_tag="VGC82_04940"
     CDS             3571..3708
                     /locus_tag="VGC82_04940"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HEY0422651.1"
                     /translation="MEKLPIVLDLTGAFVFALSGALAGVRRKLDLFGVLARLSSLGTA
                     V"
BASE COUNT          667 a         1164 c         1201 g          774 t
ORIGIN      
        1 agattcaaaa gacaatctta tgaggtgcgg cgccattcat ctggctcaca taggcgtcgt
       61 tgaaatccag ctagcggatc atcacgccat tggcgaaagc cgccagctcg gggctcgtct
      121 tcctttcgcc agccgtgcaa gagccctccg gaagtgatct gcctgacctt aggttccgga
      181 cctgagccgg caagtaactg ggggcgcgcc cgcagtttcg cgcttccact ggatttggaa
      241 gtgtggtttc gtccggttaa tcaaaaaccg attttaaggg cctcggccag tcgtctaggg
      301 agaagggaag tgcgcatttt acccctgctg cttgtggctc tatcagtgat gatcgcaccc
      361 gctgcgattg cgcagacgcc gaggatcgtc atggaggaga tgatggttcc gtcgagtgat
      421 gccgggatcg aaatcttcgt gcgcaacaag cgccccgccg acatgatctc atttcgaccg
      481 gagcgcaccc tgctgtatgt gcatggcgcc acctatccct cgtcgaccgc attcgatctc
      541 cagctcggcg gcatgtcgtg gatggattac atcgcagagc gaggttacga cgtctacctc
      601 cttgatctgc gcggctacgg caagtccaca cggccgaagg agatgaacga caagcccgaa
      661 gcaaacgcac cgatcgtgcg gggcgacacc gcagtgaagg acatttcgac cgttgtcgat
      721 ttcatcctta agcgtcgcaa catccctcgg ctggatttgc tcggctggtc gtggggcacg
      781 accctgatgg ctacctacac gacgcagaac gcggccaaag tagaacgcct ggtgctttat
      841 gcgccggcat ggatccgcac tacgccatca ctggtacagg ccggtcctgg accaattccc
      901 gcctatcgcg cggtgcgaaa ggatcaggcg ctggggcgat ggctcaccgg cgtggccgaa
      961 aacaagaagg cgagccttat tcctgcgggc tggtttgacg cctgggccga cgccacgtgg
     1021 gcgaccgacc ccgaaggcgg caagatggat cctccggtac tgcgtgcgcc caatggcgtg
     1081 gtgcaggatg gcgcagaatt ttttggcgcc agcaaggcgt attacgatcc cgccaaggtc
     1141 accgtgccaa cgctcctgat tcacgcggaa tgggaccagg acacgccgtc ctacatggcg
     1201 cagacgctgt tcccattgct ggttaactcg ccggggaaac gctacgtcca gctggctgaa
     1261 gggacccata ccatcatcat ggaaaaaaac cggcttgaac tgttcaaggc ggtgcaggcg
     1321 ttccttgatg aggcagccgg gttgtagccg gtggtcaggt cggtcggccc gaatcctccc
     1381 gtcggacctg agcaaggcgg tccttgaccg tccgtgcgat cgccgccggc gcctcgtcgg
     1441 ggatcgcgaa acaggaactc gcgttgattt cgcagagcac gtaggtgtcg gctccagccg
     1501 cgtcgcgccg gccgaacagg aaatccgcat cccaaatcac cggcagcgat gtttgatcga
     1561 tgtccagtgt ttgcatcatc tgcggcgtcc attcctcctc catcaacctt ctcagggcct
     1621 gaaacggcgg cgcctcgggg ccgtgcatga tccgaggccc aggttgagcc tccggcgaat
     1681 ccgggcccgc cgggggcggt gcgatcaggg ccttgatcag ttggtgcccg aagcccgcga
     1741 ccttcggtcc gctcatgtag cagcggatca tcccttcggg caggcgcggc tggaacgcct
     1801 gatcgatgat gcagccgccc catccgaaat acggctcgca gcggccgatg aagtcgtcga
     1861 gcggcatttg ctcaggccgg ctgccgcgct gcgcgtggag cacggtgact gtcgcgtcag
     1921 tattcggcaa cgactccacc ttccagacgc cctggcctcc gttgccgcgg ttctgtttga
     1981 gcacgcgcgg cccggacgac cgcaggcgca acgggaattc gtcacgaaag gcggcggcac
     2041 tgtcatagcg atgcgtgtcg gttccccagc cgagatggcg cgttcgatag agcacctcct
     2101 tgacgcccat cttcagtatg acgtcgggat gtgcgctcac ccacgggccg cacatcgaga
     2161 tctcgcgcag cagcggatca agcgccgcgc gcgtcttgcc ctgatggatc ggatcgaccc
     2221 agaccagaac gccgtccacg gcaagcagtt ggtcgcggac cgcgccggcg aaggtttcgt
     2281 cgtagatggc tggccgtgcc tcgatgccgg ctgcagcgag cgcctcgaag acgcggacga
     2341 agcggctgtt ttgtggggtt gtgtcccgac gtgccgcagc gtcaccgcgc gaaaggattg
     2401 cgacagtatg gccgagaggg aggtgtcgtt cggtgtccat gcgcgttctc cacgagagat
     2461 cgtgtgtcgc gcagagcttg gacggaaaag tctgacgggg agcctgccta tccccaaacc
     2521 ggactctacg ttagctcgca tccaggtttc aagatgctag ttgtttcccg tggatgcccc
     2581 ggggtaagct gaccgccggg gatcatcagg gcctccccga tagatgggtt ttccatccaa
     2641 gcgctcgcca ttctttcgag agggcgatca tggatggact atcttgggcg ttttttgcac
     2701 tggcggcctt tgcgggaggc tttgtcagcg ggttttccgg gttcgcgatg ggactcgtgg
     2761 tatccggtgc atggcttcac atcatcacgc ccctccagac cgccgccttg attgcaggat
     2821 acggcctgct cacccaaggc tacggcatca tcaagctgcg tcacgcgttt aactggcgaa
     2881 ccatcttgcc gttggcgctc ggcagcgcag tgggtattcc ggtcggcgtc tacctgctca
     2941 cctacattag tccgctcgtg cttcgcgcgg gcgtcggcgc ggtgctcatg acgtacagtg
     3001 cctacagtct gcttcggcct gcgatcggtc cctttgcgat cggaaccggc ggcgacctcg
     3061 cgattggctt cgcaaacggg atcctcggcg gaatgacagg cctgggcggc gtcatctcga
     3121 cgatctcctg tcagtggcgc gggtggccga aagacgtaca gcgcggcgta ttccagccgg
     3181 ttctgtttgc atcttttgtc gttatcgacg tttctctggg gttcgccggc gcgttcgcgc
     3241 cggagacggt cggtctctat gcggtcggcc ttccgtttct actcgccggt ctctggggcg
     3301 gtttcaaact ctatggatcg atcgatgatg agacgtttcg aaagtcggta cttctgctgc
     3361 tgctgatatc cggcctgtcg ctgatcctgt ccgccggcgg attgccaggc gcgagcggct
     3421 cgtagctgag gtcaaccatg ccggggccat agacgaagct ggtcgccagg actagacagc
     3481 cgttccaggg cttgagagcc gcgccggaac gccggttcga acggcccaat caaaagctcg
     3541 ctcgtccggt tgacgtcgga aggccgcatc atggagaagc tcccgatcgt gctcgatctg
     3601 accggcgcct tcgtcttcgc gctcagcggc gcgctggcgg gcgtcaggcg aaagctcgat
     3661 ctgttcggcg ttctggcgcg gctctcaagc cttggaacgg ctgtctagtc ctggcgacca
     3721 gcttcgtcta tggccccggc atggttgacc tcagctacga gccgctcgcg cctggcaatc
     3781 cgccggcgga caggatcagc gacagg
//
LOCUS       DASWQN010000299         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_162177, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000299 DASWQN010000000
VERSION     DASWQN010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162177"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VGC82_04945"
     CDS             complement(<1..312)
                     /locus_tag="VGC82_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422652.1"
                     /translation="MDADRRAVGKEQQIEFPAFGGLGNLHRLREHRPGFGEGIGVTPC
                     GDVLSGLVNEAPRRILRLPARLCIPMFSCPVFSWNDNSVSVSVPAGFSGHSARGVHSR
                     LP"
     gene            214..1389
                     /locus_tag="VGC82_04950"
                     /pseudo
     CDS             214..1389
                     /locus_tag="VGC82_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03860.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN.; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
BASE COUNT          290 a          458 c          509 g          264 t
ORIGIN      
        1 agggagccgg ctatggacac cacgcgcgga atggcctgaa aatccggccg gtaccgatac
       61 cgagaccgag ttgtcgttcc aggagaacac ggggcaggag aacatcggaa tgcaaagcct
      121 cgccggaagc cgcaaaatgc gcctgggcgc ctcgttcacg agaccggaca gcacgtcgcc
      181 gcatggcgtc accccgatgc cttcgccgaa tccgggacgg tgttcgcgca ggcgatggag
      241 gttgccaaga ccgccgaacg cgggaaattc gatctgctgt tccttgccga cagcgcggcg
      301 gtcagcgtcc atggcagcac ggaatcgcgt ggccgcatgg gcaagacggt gaaattcgag
      361 ccgatcacca tgctgtccgc gctggcggcg gttacgtcaa acctcggcct cgtggcgacg
      421 tgcagttcga cctacaacga gccatacacg ctggctcggc agttcgcttc gctggaccag
      481 atcagcggtg gacgagccgg atggaacctg gtgacgtcga acaacgaggc ggaagcgttc
      541 aaccacggcc tggagaagca tgcggcccac gccgatcgtt atgaccgtgc cgcggaattc
      601 gccgaggtgg tgaacgggtt gtgggatagc tgggacgagg acgccttcat ccgcgacaag
      661 acctccggca tttacttcga ccccgccaaa atgcatgtgc tgaaccatcg cggaaagcat
      721 ttcgcggtac gaggaccgct gaacgtggcg cgctcgccgc agggccggcc ggtgctggtg
      781 caggctggcg cttcagacac cggacgcaac cttgccgcgc ggctggcgga gctgattttc
      841 accgcgcaga ccacgttcga gcaggcaaca gaattctaca gcgacgtcat ggcccgcctg
      901 ccccgctttg gccgctccga gcgggacgtg aaggtcatgc ccggcctgta tcccgtggtc
      961 gggcggaccg aagcggaggc cagggaaaaa ttcgactact tgcaatcgct gattcatccc
     1021 agcgtcggca tcgcggtact ggaacacacg atcggcgtgt ccgatctgtc gcggtatccg
     1081 ctgcatggcc cggtgccgga aatgggcgac accaatggcc cactgtcgcg ccaacgcctg
     1141 ctgctggagg cggcgcgtcg ggacaagctg acgctgtggg aactgtgcct gttgaatgcc
     1201 gggccgcggg catttgctga cgatcgggac accggcgcag atcgccgatg tcatggagca
     1261 ttggttcctg aacggcgccg cggatgggtt caacgtgatg ccagcctggc tgccgggctc
     1321 gctgaccgat ttcgtcgaca tggtgattcc ggaattgcaa cggcgtggtc tgttccgcac
     1381 agattatgaa agtgccacgt tgcgcggcaa cctcggtctg ccgactccgg tctgccgctt
     1441 tcggtctgtt ccgatggtag cggaatgagc gggcagtgat acgagccaga tgggtatggc
     1501 gcgccctggt ggattcgaac c
//
LOCUS       DASWQN010000300         2933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1745628, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000300 DASWQN010000000
VERSION     DASWQN010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745628"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VGC82_04955"
     CDS             complement(<1..436)
                     /locus_tag="VGC82_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HEY0422653.1"
                     /translation="MSIVLSLLTQILHLGLMIAAAPMVAGVTDWLDARLTLRGGPPIL
                     LPWRDLVRLSRKTPMLIESVSVVSRVAPAVGFGATLSAAAMIPSFTLGMAFSPLADVL
                     VIVSLLAIARVAGALAALDSGAAVPGLMQQENCALAVLAEPAL"
     gene            complement(433..2247)
                     /locus_tag="VGC82_04960"
     CDS             complement(433..2247)
                     /locus_tag="VGC82_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422654.1"
                     /translation="MSDLTLLLLAADIVALLLLGAFGRLVPSTGSGLPAAALCGLGAL
                     LCLPVLILRLRAAALVIPIGPPGLSLHLALDPLSTIFLAIVLVAGTAVAAFQAASVRP
                     DQVSSTRMTTFCVAGVMLSLLAADGVALAIGLTVACGAIWRPRDGGLAILRVLAPLLA
                     LTSVCLLTPAGHASWFGAIRVGPADPGHATAAAVLTVVSVAGLAWPPSDRRCRTRDAL
                     IAGLLIPCGSYLLLRLIVDLADNTAWNGSGFVLLLAGGAVAIVEGWRSAADLNLDAAV
                     RALMRRQAGLAMTGIGLAMIARVSDLPGAGRFGLEAACLAAIGASLAGTLTSLAAHEI
                     GASAGTHLLSRLGGLVHTMPGTSAALGAGLLALSTLPPGIGFASLWLSFQSILSAPRG
                     GSLLAQIALALAAAALALSAALATTASVRIAGIALLGRPRTPRGAGAQEGTPLGRWIL
                     LTLGGASLLAGVLPGSVLWLLADPAIQVLIGIEPGHLNGLALLPAFDSPPRYLALPVF
                     ALLALAIGTAILVPRRSGKEAKTAGPWTDGMQPPAGLPFGEPAAQSAGAGFLPVLPKM
                     LLPRLPTLTRPRPPPASAGIWLMLAGFAVLLLVLAVSG"
     gene            2661..>2933
                     /locus_tag="VGC82_04965"
     CDS             2661..>2933
                     /locus_tag="VGC82_04965"
                     /inference="COORDINATES: protein motif:HMM:NF040013.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6166 domain-containing protein"
                     /protein_id="HEY0422655.1"
                     /translation="MKTYEGERTLRGINVTVDGAPLPPRMDLQRFNKTGFEWTYAGSG
                     PQQLALALLADHLGNDEKALSLSEAFMRHVVAYLDNAWCLTSVDIDE"
BASE COUNT          601 a          972 c         1007 g          353 t
ORIGIN      
        1 tcaatgccgg ttccgccaga accgccagcg cgcagttttc ctgctgcatc agaccgggca
       61 ccgccgcacc agaatccagg gcggccaggg ctccggcgac ccgggcaatc gccagcaggg
      121 aaacaatgac cagcacgtcg gcaagcggcg aaaatgccat cccgagggta aaggacggaa
      181 tcatggcggc ggccgacagc gtcgcgccga acccgaccgc cggcgcgacg cgcgaaacca
      241 ccgacacgct ctcgatcagc atcggggtct tgcgagacag gcggaccagg tcgcgccacg
      301 gcagcaggat gggcggtccg ccgcgcaagg tcagtcttgc gtcgagccaa tccgtcacgc
      361 ccgccaccat cggtgcggcg gcgatcatca agccaagatg caggatctgc gtgaggagcg
      421 acaggacgat gctcacccgg acaccgccag aaccaggagc aggaccgcga acccggccag
      481 catcaaccag atgccggcgc ttgccggcgg tggacggggc ctcgtgagcg tcggaagccg
      541 cggtagaagc attttgggca gcaccggcaa gaatcccgcg ccggctgact gcgcggccgg
      601 ctcgccgaac ggcaggccgg ctggcggttg catgccatcc gtccagggac ccgccgtctt
      661 ggcctctttt ccggagcggc gtggcaccag tatcgctgtc ccgatcgcca gagccaaaag
      721 agcgaacacc ggcagggcca ggtagcgcgg cggcgaatca aaggccggca gcaatgccaa
      781 tccgttcaga tgaccaggtt cgatgccaat cagcacctgg atcgccggat cggccagcaa
      841 ccacaggacc gaccccggca ggacgccggc cagcaacgag gcgccgccca gggtcagcag
      901 gatccaccga cccagtggcg tgccttcctg tgcaccggcg cctcgtgggg ttctcggtct
      961 gccgagcagc gcgataccgg cgatgcgcac ggacgcggtg gtggcgagcg ctgcggatag
     1021 cgcgagtgct gccgcggcca aagccagcgc gatttgcgcc agcaggctgc cgccgcgcgg
     1081 ggccgacagg atggactgga acgacagcca gaggctggca aaccctatgc cgggcggcag
     1141 cgtggacagc gccagcaagc ccgcgcccag ggcggccgag gttcctggca tggtatggac
     1201 caggccaccg agccgggaca gaaggtgggt tccggcgctg gcgccgatct cgtgggcagc
     1261 cagcgacgtg agagtgccgg cgaggctggc gccgatggcg gcaaggcacg ctgcttccag
     1321 cccgaagcgc cccgcgccgg gcaagtcgga cacccgggcg atcatcgcca gcccgattcc
     1381 ggtcatcgcc aaaccggcct gccgccgcat cagcgcccgc acggccgcgt caagattcag
     1441 atcggccgcc gaccgccagc cttcgacgat ggcaaccgcc ccgccggcga gcagcagcac
     1501 gaagcccgac ccattccacg cggtgttgtc ggcaagatcg acgatcagcc gcagcagcag
     1561 gtaggatccg caagggatca gcagtccggc gatcagcgca tcgcgggtcc ggcagcgccg
     1621 atccgacggc ggccaggcaa gaccagcaac cgaaacgaca gtcagcacgg cagcggcggt
     1681 cgcatggccg ggatcggcag gcccgacgcg gatggcacca aaccaggaag catgaccggc
     1741 cggcgtcagc aggcacacgg acgtcagcgc cagcaacggg gcaagaaccc gcaatatcgc
     1801 cagcccgccg tcccgcggac gccagatagc cccacaggcg acagtgaggc cgattgccaa
     1861 ggcaacgcca tcggcggcca gcagtgacaa catcactccg gcgacgcaga acgtggtcat
     1921 ccgcgtcgaa ctgacctggt ccggccgaac cgaagccgcc tggaaagccg cgacggcggt
     1981 cccggcaacg aggacgatcg ccagaaagat ggtcgaaagc ggatcgagtg ccaggtgcag
     2041 ggacagccca ggtggcccga ttgggatgac cagtgccgcg gcccgcaggc gcagtatgag
     2101 aactggcagg cacaagagtg cgcccaggcc gcatagcgcc gcagccggaa ggccgcttcc
     2161 cgtggacggc acgaggcggc caaatgcgcc cagcagcagc aaagcgacga tgtccgcggc
     2221 cagaagcaga agggtcaggt cgctcaccag cggtaggtta ccaccaccac gaggattcgc
     2281 agcgtgctcc gcgcgtggaa ggccaggatg ctccgcgtgt ggtgaagatc aagatatagt
     2341 ctgccaccat ctcaggccgg gcaccatctc gggccgggca ccatctcagg ctggattgtc
     2401 acagccgtgc cgtcggcatg gaacgaacgc aaattgaaac gccaccctgc cgggccgcgg
     2461 cgtattgatc aagaccgggc cgcaacctcg gtcgcggagt tgcgctacgg cggtaccgcc
     2521 atgatcattg gcggcgcccc gaacgttcgg tggcgcgagg ctggcgggag caatcctgtt
     2581 catggtgatg gaccacgtaa agccggcgat ggcccgggag gcagcggata aggcgatcgc
     2641 ccgcccgacg gagaaacgac atgaaaacct atgagggaga gcggactttg cgcgggatca
     2701 acgtgacggt ggacggcgcg ccgttgccgc cgcggatgga tcttcagcgc ttcaacaaaa
     2761 ccggtttcga gtggacctat gccggttcgg ggccacagca actcgccctg gcgctgctgg
     2821 cggatcatct gggcaacgac gaaaaagcac tgagcctgag cgaggcattc atgcggcacg
     2881 tggtggcgta tttggacaac gcctggtgcc tgacttcggt ggatatcgat gag
//
LOCUS       DASWQN010000301         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1745654, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000301 DASWQN010000000
VERSION     DASWQN010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745654"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VGC82_04970"
     CDS             complement(<1..1154)
                     /locus_tag="VGC82_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0422656.1"
                     /translation="MDAPCSDTIKAAVQAQRATMTALFDQLRQDGLDQPGVSRDPYGA
                     GEQRAHATVTRAAEALGLEVGHDAAANLYMTLPGRDRTAPVLVIGSHLDSVPHGGNFD
                     GAAGVLAGLATATALRNMGLTPACDIVVMAIRAEESVWFQVSYIGSRGSLGTLPDGAL
                     DVRRVDTGRTLADHIADCGGDPDALRARARHFDPKRVRAYVELHIEQAPSLVEAGLPV
                     AICTGIPGNFRFPDAWIEGRHDHVGLPRRFRRDAAMAGAEFAMALDAFWADYESRGIA
                     MACTLGRFHTDAALHGLTIIPGAFHFSLDVRAYDETVLAELVEKVYDTIKEIEWRRGV
                     TFHLGQQARAAVGPVDGDVKAGLEEAARKQDIVFLHIGSPASHDAAAFGAA"
BASE COUNT          283 a          536 c          500 g          292 t
ORIGIN      
        1 gctgcgccga aggcagccgc atcatgggac gctgggctcc cgatatgcag aaaaactata
       61 tcctgtttcc gggcggcttc ttccaacccg gccttgacgt ccccatcgac tggccctacc
      121 gcggccctgg cttgctgccc aagatgaaat gttacaccgc ggcgccattc gatttctttt
      181 atggtatcgt acaccttttc caccagttcc gccaggacag tctcatcata ggcgcgcaca
      241 tccaggctaa agtggaaggc acccggtatg atggtcaaac cgtgcaaagc ggcgtcggtg
      301 tgaaagcggc cgagtgtgca ggccattgca atgccccgcg attcgtaatc cgcccaaaac
      361 gcgtcgagag ccatggcgaa ttccgctcca gccatcgcgg catcgcggcg gaaccgtcgc
      421 ggcagcccga cgtggtcatg gcgcccttcg atccaggcgt ccggaaacct gaaattaccc
      481 gggatgccgg tgcaaattgc caccggcagc ccagcttcaa caaggcttgg tgcctgctct
      541 atatgcaatt cgacataagc gcggactcgt ttagggtcga agtgccgggc gcgggcgcgc
      601 aacgcgtcgg gatcgccccc acagtcggcg atatggtcgg ccagggtgcg gccggtatca
      661 acgcggcgga cgtccagcgc accatcaggc aacgtaccga ggctgccgcg gctgccgata
      721 tacgatacct ggaaccagac gctctcttcg gcacgaatcg ccattacgac gatgtcgcac
      781 gccggcgtca gtcccatgtt tctcagcgcc gttgccgtgg ccaatccggc cagcacgccg
      841 gctgctccat caaagtttcc gccgtgcggc accgaatcga ggtgggagcc gatgaccagc
      901 accggcgccg tccgatcacg gccgggcagg gtcatgtaca ggttggcggc ggcatcgtgg
      961 ccgacttcca gccccaaggc ttcggcggcc cgggtcacgg tcgcatgcgc ccgctgctcg
     1021 cccgcaccgt agggatcgcg ggacacgccc ggctgatcca gcccgtcctg gcggagttga
     1081 tcgaacaagg cagtcatggt cgcgcgctgt gcctggaccg cggccttgat ggtatcggaa
     1141 cacggtgcgt ccattgcttc ctccgctggt gtttggcggc ccggagcatt gtaggctttg
     1201 ccccgaacgg ccactggcag cctgtcggac ttaatgcagc gcggaaaggg gcggtagaac
     1261 cggcgcgcgg ttcgcctccc actccgggca ctggttgcat gagcccattc cgcctgatcg
     1321 accgcgacac cggcttcctg atgccaccat caatggacga atatctgccg cggcggcacc
     1381 ttggctcggc taacggtgga ggtggtcgag ggccttgatc tgccacgatg accggcagcc
     1441 accgcggctc ggtgaggcgc cctaccaccc gcaactgctg cttggcctga ccatccactt
     1501 ggcctgacca tccacggcta tccatgcgac gctgcttcct gccgatcaag acgctgttcg
     1561 tgcaggtgcg cggggccgcg cccagcgaga tggggttttg aagctgcgcg c
//
LOCUS       DASWQN010000302         3793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1217927, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000302 DASWQN010000000
VERSION     DASWQN010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217927"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VGC82_04975"
     CDS             complement(<1..277)
                     /locus_tag="VGC82_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0422657.1"
                     /translation="MASIDIKATDGSGSFTAYLLEPKAKPAGVVVLIQEIFGVNQAMR
                     DTAAWVADLGFIAVCPDLFWRIEPGIDITDKSDAEWKRAFELFQAFDQ"
     gene            complement(408..1301)
                     /locus_tag="VGC82_04980"
     CDS             complement(408..1301)
                     /locus_tag="VGC82_04980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY0422658.1"
                     /translation="MANHPIPLETVAVSVPEDALEAFESALENACDTVGFFRNHDTGI
                     WRVEGVKQVGVNEAELASSLAVAVLMTGTSPVLERSATRADGWLARTYASFPEQLIGR
                     YFAVRGTHLTGPRMPNRITLTLDAGLAFGTGEHGSTRGCLVALETMARRHPRRILDMG
                     TGSGILAMAAARLLNRKVLATDIEPWAVRVAAENAELNGVAHLVKARLADGWSDPFVR
                     AHAPYDLVFANILARPLCLMARDLVLNLAPGGTAILAGLLANQARWVLSAHRRQGLRL
                     ERMLPQGPWTTIVVRKAGAII"
     gene            complement(1301..3502)
                     /locus_tag="VGC82_04985"
     CDS             complement(1301..3502)
                     /locus_tag="VGC82_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0422659.1"
                     /translation="MSIQAPLTPGYLSRLNPEQRAAVETVDGPLLVLAGAGTGKTRVL
                     TTRFAHILLTRRAFPNQVLAVTFTNKAAREMRERVAAILGEPAEGLWLGTFHALCARM
                     LRRHAEHVGLSSNFTILDTDDQLRLLKQIMEANRIDTKRWVPAALMAAIQRWKDRGLT
                     PVRITPNEDIDFANGHATALYTAYQDRLRSLNAADFGDLILHMTEILRTRPDVLADYH
                     RRFRYILVDEYQDTNLVQYMWLRLLAQGHRNICCVGDDDQSIYSWRGAEVENILRFEK
                     DFEGAHIVRLEANYRSTASILAAAAGLIAHNEGRLGKTLRPGRIDAQAGEKVAVVELW
                     DSDEEARMVAGKIEELRRDGHSLAEMAVLVRAGFQTRAFEERLITVGIPYRVVGGLRF
                     YERAEIRDAIAYMRATVQPADDLAFERIVNVPRRGVGESALRTMHEASRAWGIPLSAA
                     AERLVETGGLKGKLKESIGTLLRSMGRWRDMLEVDGHVVTVATMLDESGYTEMWKQDK
                     SAEAPGRLENLKELVRALADFETLGGFLEHIALVMENEEQGENDKVSLMTLHGAKGLE
                     FDTVFLPGWEEGVFPNQRAMDESGNKGLEEERRLAYVGLTRARTLAVVSHAANRRIYA
                     NWQTSIPSRFLEELPDGHIEQTGGSRTNRLAAATSFSGQFPLIARAPRVIDAWEQPAR
                     PARTDKIPVGSRVFHQKFGYGTVNAVDDDKLDVRFDASGDKRVLDRFVERA"
BASE COUNT          681 a         1247 c         1159 g          706 t
ORIGIN      
        1 cctggtcgaa ggcctggaac aattcgaatg cccgcttcca ctccgcatcg gatttgtcgg
       61 taatgtcgat gccgggctcg atccgccaga acaagtccgg gcatacggcg atgaaaccga
      121 gatccgccac ccaggcggca gtgtcacgca tcgcctggtt cacgccaaaa atttcctgga
      181 taagcacgac gacgccggcg ggctttgcct ttggctccag caggtaggcg gtgaaactac
      241 cactcccgtc ggtggccttg atgtcgatgc tggccatgat ccgaccctcc ttcgtgatgt
      301 ctgccggtaa ccatacaaga cacgtcaaaa ttcgccatgg atggcggaga tgccgtctcc
      361 ggctggagaa tgtgcgagcc ttcgatacgc cgcgcgacgc ggtctgatca tatgattgcg
      421 ccggccttac ggaccacgat cgtggtccac ggaccctgcg gcagcatgcg ttccagccgc
      481 agtccctgcc ttcgatgcgc cgacagcacc caacgcgctt gatttgccag caaccccgcg
      541 aggatcgcgg tgccccctgg cgccaaattc aagacaaggt cccgcgccat caggcacagt
      601 ggccgagcca ggatattggc gaataccaga tcatacggcg cgtgggcgcg gacaaatgga
      661 tcgctccaac catccgccag tcgcgccttc acgaggtgcg ccacaccgtt cagttcggca
      721 ttctccgccg cgacccgcac tgcccacggc tcgatatcgg tcgccaacac cttcctgttc
      781 agcaatcgcg cggctgccat cgccaggatg ccggagccgg tgcccatgtc caggatccgc
      841 cgcggatgcc ggcgtgccat ggtttccagt gccaccagac agccacgcgt ggacccatgc
      901 tcgccggtcc cgaacgccag gccggcgtcc agcgtcaagg tgatcctgtt cggcatccgg
      961 ggcccggtca gatgcgtgcc acgaaccgcg aaataccggc cgatcaactg ttcggggaac
     1021 gatgcgtatg tccgcgccag ccatccgtcc gcgcgggtgg ccgaacgctc cagcaccggc
     1081 gacgttccgg tcatcaacac ggccacggca agcgacgaag ccagttcggc ctcattcacc
     1141 ccgacttgct tgacgccttc cacccgccag atgccggtgt cgtggttgcg gaagaaaccg
     1201 accgtatcgc aggcattttc caacgccgac tcgaacgcct ccagggcgtc ctccgggacg
     1261 ctgacggcga cggtttcaag cgggatcgga tggttggcca tcaggcacgc tcgacgaagc
     1321 gatcgagcac acgcttatcc cccgaggcat caaatcgcac gtccagctta tcgtcatcga
     1381 ccgcgttgac cgtgccgtag ccaaatttct gatggaacac ccgggaaccc accgggatct
     1441 tgtcggtccg tgccggccgc gccggttgct cccaggcatc gatcacccgc ggcgcccggg
     1501 cgatcaacgg gaactggccg gagaacgatg tcgccgccgc taaccggttc gtgcgtgatc
     1561 cgccggtctg ctcgatgtgg ccgtccggca gttcttccag aaaacggctg ggaatactgg
     1621 tttgccagtt ggcgtagatc cgccggttgg ccgcgtggct gacgaccgcg agcgtccgcg
     1681 cccgggtcag gccgacatag gcaagccggc gctcttcctc cagtcccttg ttgccgcttt
     1741 catccatcgc ccgctggttg gggaatacgc cttcctccca ccccggcagg aagaccgtgt
     1801 cgaactccag gcctttggcg ccgtgcaggg tcatcaggct gaccttgtcg ttctcgccct
     1861 gctcctcgtt ctccatcacc agcgcgatgt gctccaggaa accgcccagg gtctcgaagt
     1921 cggcgagtgc gcggaccagt tccttcagat tctccagccg gcccggcgct tcggcggatt
     1981 tgtcctgctt ccacatttcg gtgtagccgc tctcgtcgag catcgtggcg acggtgacga
     2041 cgtggccgtc aacctccagc atgtcccgcc agcggcccat gctgcgcaaa agggtgccga
     2101 tcgactcctt cagtttgccc ttcaggcctc cggtctccac caaacgctcc gcagccgcgg
     2161 acagcggaat cccccatgcg cgggaggctt cgtgcatcgt cctgagtgcc gactccccga
     2221 ccccgcggcg agggacgttg acgattcgct cgaacgccag atcgtcggcg ggctgcaccg
     2281 tcgcgcgcat ataggcgatg gcgtcgcgga tctcggcgcg ttcgtagaat cgcagccccc
     2341 cgaccacgcg atacggtatg cccacggtga tcagacgttc ctcgaacgca cgggtctgaa
     2401 aaccggcgcg cacgaggacg gccatctccg ccagggagtg accgtcgcgc cgcagttcct
     2461 cgatcttacc ggcgaccatg cgggcttcct catcggaatc ccacagctcc acgacggcga
     2521 ccttctctcc ggcttgcgcg tcaatccggc ccggccgcag cgtcttgccc aaacggcctt
     2581 cgttatgcgc gatcaacccg gccgccgcgg ccagaatcga ggcggttgac cggtagttcg
     2641 cctccagccg gacgatatgc gcgccctcga agtctttttc gaagcgcagg atgttttcca
     2701 cctcggcacc gcgccaggag tagatcgact ggtcgtcgtc gccgacgcag cagatgttgc
     2761 ggtggccctg ggccagcagc cgcagccaca tgtactggac caggttggtg tcctggtatt
     2821 cgtccaccag gatgtagcgg aagcgccggt ggtagtcggc cagcacgtcg ggccgggtcc
     2881 gcagaatctc cgtcatatgc aggatgaggt cgccgaaatc ggcagcgttg aggctgcgca
     2941 atcggtcctg gtaggccgtg tacagcgccg tagcgtggcc attggcgaag tcgatatcct
     3001 cattcggcgt aatccgaact ggcgtaaggc cgcgatcctt ccagcgctgg atggccgcca
     3061 tcagcgccgc cggtacccaa cgtttagtgt cgattcggtt cgcctccatg atctgcttca
     3121 gcaggcgaag ctggtcgtcg gtgtccagaa tggtgaagtt cgaagacagg ccaacgtgtt
     3181 ccgcgtgacg ccgcagcatc cgggcacata aggcgtggaa ggtgccgagc cacagacctt
     3241 cggctggttc gccaaggatg gcagcgaccc gctcgcgcat ttcccgcgcg gccttgttgg
     3301 tgaaggtcac ggccaggact tggttgggaa aggcgcgcct ggtcagcagg atgtgggcga
     3361 agcgggtggt cagcacgcgc gtcttgcctg tgccggcccc cgcgaggacc agcaacggac
     3421 catcgacggt ttcgacggcg gcccgctgtt cagggttcag gcgggacagg tagccagggg
     3481 taagcggagc ttgaatcgac acgagctgaa tatagggcag ttcccggtgg acgggaacgt
     3541 ctgttctatc cgaacgcggc cggactgtcg gaggatttta cacaagatgg gtgaggccgg
     3601 gtaagccggt cgctaaccgg ttggaattga ggctcgcgtc acgttggcac aacgattgct
     3661 aatcgttgca gtgagcaatc ctggtatggt cggacgttat gaaaactttc ctgattacgc
     3721 attatcctca gccgaatttg gacatcgcac caaccgcgca aaatcaacgt tgtaggggtt
     3781 tcttcaaagc ccc
//
LOCUS       DASWQN010000303         5467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2405103, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000303 DASWQN010000000
VERSION     DASWQN010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405103"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="VGC82_04990"
     CDS             complement(<1..1422)
                     /locus_tag="VGC82_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422660.1"
                     /translation="MTVILSAGLVRCTGVSASQDERRIDRARPTTVERKGRSPTSKIK
                     AGAEALGKQGWLPNVVSMSRRSVLAGSVSVAATGALSRPYIANAQAKTAVCWLNQGFI
                     PQEDAAMKAVAEEYMKASGNKLEYSVMPFMALNQKTISALTSGDVPDLVFMDAPSSIL
                     PQTAWEDKLVDVSDVVSSYESQLTDTAKMCSTFYNKATKKRSYYLCPIKQGATPFHVW
                     GDLVEKAGMKLSEAPNTWDALWNFLAETQQPLRAKGMRKIYALGLQVTTVGPNDGNNL
                     FAHFMIANGGQNIVTPDGKLHTDDPQVLEAAIKSVTFMTDLYKRAVVPPEALSWNDAD
                     DNNGFHEKLFVADFDGTLSTELAMIKDKKAYNDMVTLGLPNKNDGTPMPCQVNAGGGY
                     IPKGAKNIEVAKDFMKYFMQPKVMNENLKAGLGRWVPAIGSLVKDDPWWLDKSDPHRV
                     AYVTEASIGPTIPVYEGFTPAWGQ"
     gene            1552..2082
                     /locus_tag="VGC82_04995"
     CDS             1552..2082
                     /locus_tag="VGC82_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0422661.1"
                     /translation="MKDDDTASPPALDEETIAFAGRVFNCARAGQAAELGELLRMGLP
                     ANLRNDKGDTLLMLASYHEHAECTRLLLEANADPEIANDRGQTPLAAACFKGNQEIVR
                     LLLRHGAQVDGCGETGRTALMMAAMFNRSEIVELLLEQNADIGARDASGKTARDLAEA
                     ISAPDTPAQLLRAAMR"
     gene            complement(2229..2903)
                     /gene="tenA"
                     /locus_tag="VGC82_05000"
     CDS             complement(2229..2903)
                     /gene="tenA"
                     /locus_tag="VGC82_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEY0422662.1"
                     /translation="MTDSFTETMRLECSAIWSAQLAHPFVVALADGTLPPETFRFYIL
                     QDARFLTELAKTFAFAATKTDDRDRILKFGELLMDTVRVERGLHEMYAARFGLTVAQM
                     AATPMAPTNYAYTRHMLQIAAAGSLAEVVTVMLPCAWIYSVIGEHFTKLGEPPADHPY
                     RDWLAMYANPDFAAVGAWLRAVVDEEASRLDDHDRQRLRDIFRTSSDYEWLFWQMAWT
                     REAWPR"
     gene            3133..4176
                     /locus_tag="VGC82_05005"
     CDS             3133..4176
                     /locus_tag="VGC82_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422663.1"
                     /translation="MNADQVGHIFKQALATFGPGRPLILTHNDADGLSAGALLARSLA
                     GIGRPADIRVLSRGENPWSADMARELAAQLPAGIVVADLGVRAGAIRGDTPTVVIDHH
                     VPTGSAGGATVITGFGADPTPTTSLLAWWCAIGLNVPDDLFWLAAIGLIGDLGDRAPF
                     QILTEARKRYGATVLREAVSLLNAPRRAALGDAAPAMALLMKADGPRAVISGEHPETA
                     LLIAARTEVKQALEAAKRIPPKIKPPVALIRLHSPCQIHPLVAQAWKARLKDSIVMAA
                     NTGYRAGWVHFSVRSATGRDLVEFLRDHAPDGADEQYGSGHQQATGGALRPAAWNAFI
                     AGLGFAHEMQVAA"
     gene            4173..5204
                     /gene="uvsE"
                     /locus_tag="VGC82_05010"
     CDS             4173..5204
                     /gene="uvsE"
                     /locus_tag="VGC82_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HEY0422664.1"
                     /translation="MTPLKLGFPVKVMARSDLKSNDTRRWQSNPHLRTSLEYLDAIFD
                     HLDKHDIRMYRMSSDLAPYATHPDMAQFHGMIGESAAELAAAGAKARRLDIRLSFHPS
                     QFVVLNSPDEELVRKSTWDLLSQAEMLDKMELGPEAVMVIHVGGHYGDRPASRERWAR
                     VWPTLPEPVRRRLVLEHDDIRFSAADVLWIHEQTGVRLIFDHQHFWCLNPEGLDLRHT
                     LTAILATWSGTERPKVHFSTPNNGMRELKRRDRKTGKIQQVAVAPVWTGHADFCSPFE
                     FAAFMRIAEGLDFDVMLESKVKDLALIRLRLDLLRYAPDVGARFGLTPDKQPALEQEE
                     AEVLEEEPA"
BASE COUNT         1063 a         1676 c         1652 g         1076 t
ORIGIN      
        1 ctggccccag gcaggagtaa agccttcata gaccgggatc gtcggaccga tcgacgcttc
       61 ggttacatac gcaacgcgat gcggatcgga cttgtcaagc caccaagggt catccttcac
      121 gagcgatccg atcgccggga cccaacggcc gaggccggcc ttcaggtttt cgttcatcac
      181 cttcggctgc atgaaatact tcatgaagtc cttggcgact tcgatgttct tcgccccctt
      241 cgggatgtag ccgccgccgg cgttgacctg gcagggcatc ggcgtgccgt cgtttttgtt
      301 cggcagaccg agcgttacca tatcgttgta ggctttttta tccttgatca tcgccagttc
      361 ggtcgagagc gtgccgtcga agtccgccac gaacagcttt tcgtgaaagc cattattgtc
      421 gtcggcatcg ttccagctca gcgcttccgg cggaacaacc gcacgcttgt agaggtccgt
      481 catgaaggtg accgacttga tcgccgcctc cagaacctga gggtcgtcgg tgtgcagctt
      541 gccgtcgggc gtgacgatgt tctgaccgcc gttggcgatc atgaaatgcg cgaacaggtt
      601 gttgccgtca tttggtccga ccgttgttac ctgcaggccg agcgcgtaga tcttgcgcat
      661 gcccttggcg cgcagcggct gttgcgtctc agccaagaag ttccacagtg catcccaggt
      721 attcggtgcc tccgacaatt tcatgcctgc tttctcgacc aggtcacccc agacatggaa
      781 cggggtggcg ccctgcttga tcgggcaaag gtagtagctg cgcttcttcg tggccttgtt
      841 gtaaaaggtt gagcacatct tcgcagtgtc ggtgagttgg gattcgtaac tggacacgac
      901 atcgctgacg tccaccagtt tgtcttccca cgcggtctgc ggcaggatcg acgaaggcgc
      961 gtccatgaaa accaggtccg gcacgtcgcc gctggtcagc gcggatatgg tcttctggtt
     1021 tagcgccatg aacggcatca cactgtattc gagcttgttg ccgctggctt tcatgtattc
     1081 ctcggccacc gctttcatcg cggcgtcctc ctgaggaata aagccctgat ttagccaaca
     1141 aactgccgtt ttcgcctggg cattggcgat atagggccgc gagagagccc ccgtggccgc
     1201 aacgctcacg gatccagcaa gcacggaacg ccggctcatg ctaaccacgt ttggtaacca
     1261 tccctgtttg cccagtgctt cggcaccggc ttttattttt gacgtcggac tccgaccctt
     1321 tcgttctacg gtagttggcc gggcgcggtc aatcctgcgt tcatcttgag aagccgacac
     1381 gccagtgcat cgaaccaaac ccgcggacag aatgaccgtc acgaggtgat cttcggactg
     1441 atatgcttgc acgtgaaatg acggtcggtt cactcattga ttatttgtcc ccgaccatgg
     1501 aacggcaaat tccgcttgct gttcaggata gcctcccgat caggacacga gatgaaggac
     1561 gacgataccg cctccccacc ggccctcgac gaagagacca tcgcattcgc cggacgggtc
     1621 ttcaactgcg cccgggccgg ccaggcggcg gagcttggcg agttgcttcg catgggactg
     1681 ccggccaacc tgcgcaacga caaaggcgac acgctgctca tgctggcaag ctatcacgag
     1741 cacgcggagt gcacgcgcct cctgctggag gcaaatgccg accccgagat cgccaacgat
     1801 cgggggcaga caccactggc cgctgcctgc ttcaaaggaa accaggagat cgtgcgattg
     1861 ctgctccggc acggagccca ggtcgatggg tgtggcgaga ctggccggac ggcgctgatg
     1921 atggctgcca tgttcaaccg ctccgagatt gtcgagctgc tgctggagca gaacgcggac
     1981 atcggtgccc gtgacgcatc cggaaaaacc gctcgggatc tggcggaagc aataagcgca
     2041 cccgacacgc cggcacaact cctgcgcgcg gcgatgcgct aagatcatgc tcagcgccga
     2101 agcgcttcag gagccctgac atccataggt tgcctttgct ttacaaaatc gggacagctc
     2161 ggatcatgat cccctgccct tgcgaggtcc caacccctgg gcgcaccacg ggcgccctcg
     2221 gaattcgact accttggcca ggcttcgcgg gtccacgcca tctgccagaa aagccattcg
     2281 taatcgctgc tcgtgcggaa gatgtcgcgc agcctttggc ggtcgtgatc gtccagtcgg
     2341 gatgcttcct catcgacgac cgcgcgcaac cacgcgccca cggccgcgaa gtccgggttc
     2401 gcgtacatcg ccagccagtc acgatacgga tggtccgcgg gcggttctcc cagcttggtg
     2461 aaatgctctc caatgaccga atagatccag gcacagggaa gcatcacggt gacgacctcc
     2521 gcgagtgacc cagccgcggc gatctggagc atgtggcgcg tatatgcgta gttcgtcggc
     2581 gccatgggtg tggcagccat ctgcgccacc gtcaggccga agcgcgcggc gtacatctca
     2641 tggaggcccc gttccacccg cactgtatcc atgaggagtt cgccgaactt cagtatgcgg
     2701 tcgcggtcgt ccgtttttgt cgcggcgaag gcgaacgttt ttgccaactc ggtcagaaag
     2761 cgggcatcct gcaggatgta gaaccgaaac gtctccggcg gcaaagttcc gtccgcgagc
     2821 gccacgacga acggatgcgc cagttgcgcg gaccagatcg cggagcattc aagccgcatc
     2881 gtctcggtga acgaatcagt cacgatacaa taccccgcgt ccatggacga atgtctatca
     2941 gtcttgaccg gcttgctcgg ggacggccag agcttgaacg gtacacttgt cgagtatgat
     3001 tcccaaatcg cgagaggctg gcctcgccca gcttatgcca agatcgcggg acgcccatgc
     3061 gaaacccaaa ggcgatcgga cattgagcca ggatgctggc aacgcgtgtc atcttgccgg
     3121 gttaaaccag gcatgaacgc agatcaggtt ggacacattt tcaaacaggc tcttgcgact
     3181 ttcggtccag gaaggccgct catcctgacc cacaacgacg ccgacgggct gtctgccggt
     3241 gctctgctgg cgcgctcact ggcaggcatc ggccggccag ccgacatccg cgtgctcagt
     3301 cgcggcgaga acccgtggtc ggctgatatg gcccgtgaac tcgccgctca attgccggcc
     3361 gggatcgtgg tggcggattt gggtgtgcgt gccggcgcca tccgcgggga tacgccgacc
     3421 gtggtcatcg accaccatgt cccgacgggc agcgccggcg gtgcgacggt gatcaccggt
     3481 ttcggtgccg atccaacgcc tacgaccagc ctgttggctt ggtggtgcgc catcggattg
     3541 aacgttccgg acgatctgtt ctggctggct gcgatcggcc tgatcggaga tctgggcgac
     3601 agggcgcctt tccagattct gaccgaagcc agaaagcgct acggcgccac cgtcctgcga
     3661 gaagccgtgt cgctgttgaa cgcaccccgc cgcgccgcac tcggcgacgc ggcaccggcc
     3721 atggcgcttc tgatgaaggc cgacggaccc agggcggtga tttcgggcga gcatcccgaa
     3781 acggcgcttc ttatcgcggc acgaaccgaa gtgaagcaag ccctggaggc agcaaagcgc
     3841 atcccgccga aaatcaagcc tccggtggcg ctgatccgat tgcactcgcc gtgccagatc
     3901 catccacttg tcgcgcaagc ctggaaggcg cggctcaagg acagtatcgt tatggccgcc
     3961 aacactggct acagagccgg ctgggtccat ttttccgtgc gcagcgccac tgggcgggat
     4021 cttgtggaat ttctccgtga ccatgccccc gacggagccg acgaacaata cggcagcggt
     4081 catcagcagg cgacaggagg tgctttgcgg ccggctgcct ggaatgcgtt catcgccgga
     4141 ctgggctttg cccacgaaat gcaggtggcg gcatgactcc gctgaagctc ggttttccgg
     4201 tcaaggtcat ggctcgatcc gaccttaaga gcaacgacac ccggcgttgg cagagcaacc
     4261 cgcatctgcg caccagcctg gagtacctgg acgccatctt cgaccatttg gacaagcacg
     4321 acatccggat gtaccgcatg tcgtcagacc tcgcgcctta tgcgacccat cctgatatgg
     4381 cccagtttca cggtatgatt ggcgaaagcg ccgccgagct ggccgcggca ggtgcgaagg
     4441 cgcgccggct tgacatacgt ctgtcattcc atccgtccca gttcgtagtg ctgaacagcc
     4501 ctgatgagga gctggtgcgc aaaagcacgt gggatctcct aagccaggcc gagatgctgg
     4561 acaaaatgga gttgggaccg gaagcggtca tggtgatcca tgtcggcggc cactacggcg
     4621 accgccccgc cagtcgggag cggtgggcgc gggtttggcc caccttgcct gagccagtac
     4681 gccgcagact ggtgctggaa catgacgaca tccgtttcag cgcggccgac gtactatgga
     4741 tccatgagca gacgggcgtc cgcctcattt tcgatcatca gcacttctgg tgcctgaacc
     4801 ccgaaggact ggacctgcgg catacgctaa cagccatcct cgccacatgg tccggcacgg
     4861 aacggccgaa agtgcatttc tccacaccta acaatgggat gcgggagcta aaacgacgcg
     4921 accggaagac tggtaagatc cagcaagtcg cagtggcccc ggtttggacg ggacatgccg
     4981 acttctgcag cccgttcgag ttcgctgcct tcatgcgtat cgccgagggg cttgatttcg
     5041 acgtcatgct ggaaagcaag gtgaaggatc ttgccttgat ccgtctccgc ctcgatctgc
     5101 tgcgctacgc cccggatgtc ggcgcccggt ttgggcttac gcctgataaa cagccggcgc
     5161 tggaacagga ggaggcagaa gtgctggagg aggaaccagc gtaaatttgc ttatgataag
     5221 tcaccgccgg gcaggcttgt cccgcgcaac tctacagcga atggcgccga ctcggttttc
     5281 cagatgccaa ctctgggcga gaggccagcc cgagtgggat gccagccttg ggtgggagga
     5341 tcagttaaat ctaaacaatc atgatctaaa gcgagatgac ctccatcatc ccggtagaat
     5401 gcccagccga tcgccgacgt gtcggccaac gatctgtccg gttcggcctg aacctgggac
     5461 cggcttt
//
LOCUS       DASWQN010000304         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2405191, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000304 DASWQN010000000
VERSION     DASWQN010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405191"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VGC82_05015"
     CDS             <1..357
                     /locus_tag="VGC82_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY0422665.1"
                     /translation="ELYRAVSAVKAELSRSATAVLRFRHADFAIEKPIARADFEHWIA
                     PDIQRIATAVDLALARAGVGEDEVDRVFLTGGTSLVPAVRGLFIPRFGAHRVTGGGEF
                     VSVAEGLALIGRDRSG"
     gene            559..999
                     /locus_tag="VGC82_05020"
     CDS             559..999
                     /locus_tag="VGC82_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422666.1"
                     /translation="MRIRQASVLMTSMIALAVSSLAGCTLEQILIGQWYTIDTPSAGG
                     CPGLEWRFVVSPQRSIDGFLSRGGQQPIAKLAGVLNPDDSFQITATEMAGGRTADVTG
                     QFTSQVTTISIHGSAAGSACDGQTFKLRLGSYFRTQGGGGGGRG"
     gene            complement(1039..1935)
                     /locus_tag="VGC82_05025"
     CDS             complement(1039..1935)
                     /locus_tag="VGC82_05025"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0422667.1"
                     /translation="MRYRHTSQTGASPKVVVGIQPPQGRPKPPGQAARNQPVALAISA
                     LAHVVAACVLLVWLQSPVPPPAVEQTVELTFEPPAEPAPQPSPETQPAETPNQPAEPA
                     AVAPALPQEPAPPAQEPPSEPAPAPVPVAPPPPIQPEEPPKLPPKLPTVEPDERPTPP
                     PPPPRPRSRPVAQPAPATPAPPNTPVAPPSGSSPAPTEAPPAPVSDVWRQALSAWLAA
                     HKTYPEAARRTGAEGAVTLRFTVSRSGHVLDVALVRSAGSPVLDAAAEAMLRSATLPS
                     FPAGMPQDSITATVQIRYTLTN"
BASE COUNT          366 a          705 c          825 g          427 t
ORIGIN      
        1 gaactgtatc gagccgtgtc cgccgtgaag gccgaattgt cccgttcggc gacggccgtg
       61 ctgcggttcc gccacgccga cttcgcgatc gagaagccga ttgcccgggc ggatttcgaa
      121 cattggatcg cgcccgatat ccaacggatc gccacggccg tggatctggc actggctcgc
      181 gccggggtgg gcgaggatga agtggatcgc gtattcctga ccggtggaac atcgctggtt
      241 ccagcggtgc gaggactgtt catcccacgc ttcggcgccc accgggtaac gggcggagga
      301 gaattcgtgt cggtggccga gggtctggca ctgatcggac gcgaccggtc gggataattc
      361 gatcggcgtc cggacggcgc tgatatcgcc ggcattaccg cggctgatgc ctatcgggtg
      421 acgttggatt gatttaaagt ccagccgatt atcgggccaa gatgacaaag ccgtcgattc
      481 ggtgccatcc gcatcagctt acgcaacatc cggggtcgcc ggaacattga cggcccggcc
      541 ggcggagaac actcgaacat gcggataaga caagccagcg tgctcatgac cagcatgatc
      601 gcgcttgccg taagttcctt ggcgggatgc acgctggagc aaatactgat cggccagtgg
      661 tacaccatcg atacgccgtc ggcaggtggc tgcccgggcc ttgaatggcg gttcgtggtc
      721 agcccccagc gctcgatcga cggattcctg tctcggggcg ggcagcagcc gattgccaaa
      781 ctggcgggcg tattaaaccc ggatgacagc tttcagatca cggcaaccga aatggcggga
      841 ggccggacgg ccgacgtgac cgggcagttc acgtcgcagg tgaccacaat ttcgattcac
      901 ggcagtgcgg cgggaagcgc atgtgacgga cagaccttca aattgcggtt ggggagttac
      961 tttcggacgc agggtggcgg gggcggcggg cgtggttgat ctgccatcgc cacgcgcgat
     1021 cggacaatag aggccagctc aattggtcag ggtgtagcgg atctggacgg tcgcggtgat
     1081 gctgtcctgc ggcatacccg cgggaaacga aggcaatgtc gcgctgcgca acatggcttc
     1141 cgccgcggcg tccagcaccg gggagccggc actgcgcacc agcgcgacat ccagtacatg
     1201 ccctgaacgg ctaacggtga atcgcagcgt tacagcgcct tccgcgccgg tgcgcctcgc
     1261 cgcctcggga taggtcttat gggccgcgag ccaggccgag agcgcttgcc gccaaacgtc
     1321 gctgacaggt gcggggggcg cttccgttgg tgccggtgac gaccctgatg gcggcgcgac
     1381 cggcgtattc ggtggtgccg gcgtggcggg cgcgggctgc gccaccggcc gagaccgcgg
     1441 acgcggcgga ggcggcggtg gggtgggcct ttcgtctggc tccaccgttg gcagcttagg
     1501 gggcagcttc ggcggctcct ccggctggat cggcggcggc ggggcgacgg gtactggtgc
     1561 tggtgctggt tcggatggcg gttcctgtgc cggcggcgca ggctcttgcg gcagcgcggg
     1621 cgcgacggcc gccggttcag ccggttggtt tggcgtctcc gccggttggg tttcgggcga
     1681 gggctgtggc gctggttccg ccggtggttc gaacgtcaat tcgacggttt gttctaccgc
     1741 aggcggcggc accgggcttt gaagccaaac cagcaacacg cacgcggcaa cgacatgcgc
     1801 cagcgcggaa atcgccaggg caaccggctg gttgcgtgcc gcttggccag ggggttttgg
     1861 gcgcccctgc ggcggctgga ttccgacaac gacctttggt gacgcgccgg tttgcgacgt
     1921 gtgccgatat cgcatgttgt gtacgatatc ggacgccgtg tcgccgcttc gcggcccttt
     1981 gcgaaggaga ttttcatcaa tgttccgccg ccgcatcttg gtgtccgggt gcctcgccat
     2041 gaccggggtc acgaactccg cattccgcgc cgacacaagc cccggcggcc gcggaattcg
     2101 atcgatatcg acgtcattcc ttgccggctc ggtgcggcga cagcagattc agcttagccc
     2161 ggacgccggc gtctatcgcg ttccccgcaa gcgctggaac ggttccgtgc cgatacgcgc
     2221 ccgattcggt cttgttgccc cccgcgttgt ccgggcagtt tcggccaggt gcatctgcgc
     2281 ggcgagcatg ccaacagcca gttccgcctg aacggcgccg gcc
//
LOCUS       DASWQN010000305         6157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_426339, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000305 DASWQN010000000
VERSION     DASWQN010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426339"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1048
                     /locus_tag="VGC82_05030"
     CDS             89..1048
                     /locus_tag="VGC82_05030"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY0422668.1"
                     /translation="MAVYTDVTDDALTAFLLDYDIGTVVAFRGVTEGVENSNYSLRTT
                     GGDFFLTLYEKRVDPNDLPWFLGLMEHLARRGLVCPQPVRGRDGLALRHLCGKHAAVT
                     TFLPGVWPRRVRQSHCAPVGEALARLHLAGEDYAPTRANALGPHSWMPLLERCRARAN
                     EVQADLTTELTNTMGAILGGWPDKLPVGHIHADMFPDNVFFLDGKLSGVIDFYFAATD
                     LLAYDVAVCLNAWCFESDYSFNVTKSRALLGAYQAVRQLSEEEQAALPVLCQGAAIRF
                     LLTRLFDWVNTPADAIVTRKDPIEYLRRLRFHLSATDGRAYGL"
     gene            1038..1532
                     /gene="rnhA"
                     /locus_tag="VGC82_05035"
     CDS             1038..1532
                     /gene="rnhA"
                     /locus_tag="VGC82_05035"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY0422669.1"
                     /translation="MASDDIVVSLPPDPDKVVEIWTDGGCKPNPGPGGWSAILTFRNV
                     VKELSGAEPATTNNRMELTAAAEALESLKRPCHVVVHTDSQYLRNGITRWSTGWVRKG
                     WRSSTGDPVANIDLWKRILAAVATHRVEWRWVRGHDGNHMNERADRLATEARRAAFGG
                     EQPG"
     gene            complement(1534..2268)
                     /locus_tag="VGC82_05040"
     CDS             complement(1534..2268)
                     /locus_tag="VGC82_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY0422670.1"
                     /translation="MIRLCIAGFMLLTIVQRCDNALAAEFSPGQRAEIVGIMRDALKQ
                     DPSIMRDAIVALQADEGARTQSATRAAITQAHEQLPAPGDPVVGDAHGDVTIVEFFDT
                     RCPYCRKLEPVMDSFLAKDRRVRLVYKDLPILGPASILGSKALLAAQKQDAYVRMRDA
                     VMKLPPDTTLAQIQATATGLGLDWPRMARDMDDPAVQARIDANLKLAHDLGIQGTPAL
                     IIENELVPGAVDLPELEKMVGEARRG"
     gene            2480..4003
                     /locus_tag="VGC82_05045"
     CDS             2480..4003
                     /locus_tag="VGC82_05045"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY0422671.1"
                     /translation="MRRGTINSLPQTSALPPEQRASFKRQIGNRPFNRWLKPAALGLL
                     ALIPACSTVGDVTNSVLGTGGPSRGQPGYVRGFLGGVVADDPRAVLAGREVLSAGGNA
                     ADAAVAMGLTLTVTLPSRAGLGGGGACLAYAPDASSPGGGKPEAVLFVPLAPATTGRG
                     ADRPAAVPMMARGLFLLHARYGSTPFETLISSAEQLARFGVPTSRALAKDIAAVSGPL
                     LADPGARAVFSQNGVPLTEGQPLRQPDLGGTLSQLRIAGVGDLYQGTLARRVALVSQE
                     IGGPIQLSDLRGGLPKLAAPLVRQYGNDRVAFLPPPADGGLATAAAFDDLANNPNDTA
                     GAVARYLSVLARYRAGGISATEVLAAQGLPAVGAPPFPASTSLVAMDRSGNAVACALT
                     MDNLFGTGRIMPGLGILAAASPAAVQPPLLSAGMVWNDNIHAFRAAAGGSGQAGAPLA
                     VAFALINTLKTGRPLSTPVPDPGRANVIACSRYLPGETSECGWANDPREAGLAMGAN"
     gene            4015..4329
                     /locus_tag="VGC82_05050"
     CDS             4015..4329
                     /locus_tag="VGC82_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422672.1"
                     /translation="MRLLASGCALVAAASAHAAFAGDTASGATSIGPGRIVCNAAFCQ
                     LGSGARPKERFRVIVSDLPREEIHRLRKCTGVAKPCIVAIDGVEQGSPMRIMATSIHW
                     QD"
     gene            4334..5494
                     /locus_tag="VGC82_05055"
     CDS             4334..5494
                     /locus_tag="VGC82_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0422673.1"
                     /translation="MAIKIGKGAAAPPFLVMDVIAAANARQAALAAGAPHVLRMEVGQ
                     PGTGAPAGAVAAAQAALRSGHPLGYTEAFGLRSLRHRIAAHAGDWYGLKLPIERIAVT
                     VGASGAFPLAFLAAFDPGDRIALAAPFYPPYVNILTALGMIPVILEAGLETRFQPSVA
                     MLEALEPPPDGLIVASPCNPAGTMLHRDELAAIATWCDRNGVRLISDEIYHGLNYDSP
                     VASAAEFSDSAIVVNSFSKYFSMTGWRIGWLVLPPDLTRPVECLAQNMFISAPYISQV
                     AAEAAFDCHDELRANVARYRQSRDHLLAALPACGFSRLSPAEGAFYLFADISERSNDS
                     VAFCDRMLREAGVAVSPGVDFDRTRGNRFVRFSYCGAEEDMRAAAERLRAWR"
     gene            5789..>6157
                     /locus_tag="VGC82_05060"
     CDS             5789..>6157
                     /locus_tag="VGC82_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422674.1"
                     /translation="MTWISNAKALLGRWWIMREARAIERRNNEEPYWVFEGMINEARD
                     VLDRGNRVRAREVWVQASAMFPAMAMMSTKALDFLLRLQAYDEADALVAKTRKQYPHR
                     AHSLEGLAKVALKRGYHEEAV"
BASE COUNT         1048 a         1949 c         2041 g         1119 t
ORIGIN      
        1 cccagactcg ccggatcaag ccaaaggcct attccgacaa cttcaagtat aaccgcattg
       61 aaaactggac gcactgaccg gcgaaccgat ggcggtctat accgatgtca ccgacgacgc
      121 gctgacggcg ttcctgctgg actacgatat cggcacggtg gtcgccttcc gcggcgttac
      181 cgagggtgtc gagaattcta attattcgct ccgcaccacc gggggcgatt tcttcctgac
      241 gctgtatgag aagcgcgtcg atccgaacga tcttccgtgg ttcctagggc tgatggagca
      301 tctggcgcgg cgcggcctgg tctgcccgca gccggtacgc ggacgcgacg gattggcgct
      361 gcggcatttg tgcgggaaac atgcggccgt caccacgttc ctgcccggag tctggccccg
      421 gcgggtgcgc cagtcccatt gcgccccggt cggggaagcg ctcgcccggc tgcacctcgc
      481 gggtgaggac tacgcaccga ccagggcaaa cgcgctcgga ccgcatagct ggatgccatt
      541 gctggagcgc tgccgtgccc gtgccaatga ggtgcaggcg gatctgacga cggaactgac
      601 caacaccatg ggcgcgatcc ttggcgggtg gcctgacaaa ttacccgtag gacatattca
      661 tgccgatatg ttccctgaca acgtgttctt tctggacgga aagctgtccg gcgtgatcga
      721 cttctacttc gctgccacgg acctgctggc gtacgacgtg gcggtctgcc tgaacgcttg
      781 gtgcttcgag tctgattact cgtttaacgt aacgaaaagc cgggcactgc tgggggcata
      841 ccaggcggtg cggcagctat cggaggagga gcaggcagcg ctgccggtac tgtgtcaggg
      901 agcggcgatc cgtttcctac tgaccaggct attcgactgg gtcaatacgc cggctgatgc
      961 aatcgtgaca cgaaaggacc cgatcgagta tttgcggcgt ttgcggtttc acctgtcagc
     1021 tacggacgga cgcgcttatg gcctctgacg acatcgtcgt gtcgttgccg cccgatcccg
     1081 acaaggtggt cgagatctgg accgatggcg gctgcaagcc gaaccccggc cctggcggct
     1141 ggtcggcgat cctgacattc cggaatgtgg tcaaggaact gtcgggagcc gaacccgcca
     1201 ccaccaataa tcgcatggaa ctgaccgcgg cggccgaagc actggagtcg ctcaagcggc
     1261 cgtgccatgt ggtggttcat accgacagtc agtatctgcg gaacggcatc acgcgctgga
     1321 gcacgggctg ggtgcgcaaa ggctggcgga gctcgaccgg ggatccggtg gcgaacatcg
     1381 acctctggaa gcgcatcctg gcggctgtgg caacgcacag ggtggagtgg cgatgggtgc
     1441 gcggacatga cggcaaccac atgaacgagc gtgccgaccg gctggcgacg gaggctcggc
     1501 gggcagcatt tggcggtgag cagcccgggt aaactacccg cgccgcgcct ccccgaccat
     1561 cttctcgagc tccggcagat ccaccgcccc cggcaccagt tcgttctcga taatcagcgc
     1621 cggcgtcccc tggattccca gatcgtgcgc caacttcaaa ttcgcatcga tccgggcctg
     1681 caccgccgga tcatccatat cccgagccat ccgcggccaa tccaggccta atcccgtggc
     1741 ggtcgcttgg atctgcgcga gcgtcgtgtc cggcggcagt ttcatcaccg catctcgcat
     1801 tcggacatag gcgtcctgct tctgagccgc cagcagcgcc ttgcttccca ggatgctggc
     1861 cggccccagg atcggcaaat ccttgtaaac cagccggact cggcgatcct tggccaggaa
     1921 gctatccatc accggctcca gcttgcggca gtacgggcag cgcgtgtcga aaaactccac
     1981 gatcgtgacg tcgccatggg catcgcccac gacagggtcg ccgggagcgg gcaactgttc
     2041 atgcgcctga gtgatggccg cccgcgtcgc cgactgggtc cgtgccccct catcggcctg
     2101 tagtgccacg atcgcgtccc gcatgatcga cggatcctgc ttcagcgcgt cgcgcatgat
     2161 cccgacgatt tccgcccgct gaccgggcga aaactcggcg gccagggcgt tgtcgcacct
     2221 ttgcacgatg gtcaggagca tgaagccggc aatgcacaga cgaatcatat tcggtccttg
     2281 tcaggaggca acggcgattc tcataagcct gcatacaatg tccatggcaa gtcaaagtgg
     2341 cgagcgagcg ccgaggctgg ttgccgctga tgccgaagcc gtctaaggca cccaaccaat
     2401 cggcaaggcg cggccccggg agagtggcaa tcccaagtct ggcgcacccg aggattgcag
     2461 acacggcagg atggatggca tgcgtcgcgg cacgatcaac agcttaccgc agacttcggc
     2521 gctgccgccc gaacagcggg cctccttcaa gcggcagatc gggaatcggc cgtttaatcg
     2581 gtggctgaag ccggcggcgt tggggttgct tgcgctaatt ccggcgtgct ccaccgtcgg
     2641 cgatgtcacc aattcggtcc ttggcaccgg cggcccatcc cgcggccagc ctggctatgt
     2701 ccgtggcttc ctgggcggag tggtcgccga cgatccgcgc gccgtactgg ccggccgcga
     2761 ggtgctgtcc gccggtggca atgccgccga cgccgctgtc gcgatgggcc tgaccctgac
     2821 ggtcacgttg ccttctcgcg ccggcctggg cggcgggggc gcctgcctgg cttacgcccc
     2881 cgatgccagc tctcccggcg gaggcaagcc cgaagcggtg ctgttcgtgc cgctcgcccc
     2941 ggcgacgaca ggacgcggcg cggatcgccc ggccgcggtg ccgatgatgg cgcgcggact
     3001 gttcctgctg catgcccgct acggctcgac cccgttcgag acgctgatct cgtcggccga
     3061 acaattggcg cgcttcggcg ttccaacatc gcgtgccctg gcgaaggaca tcgccgcggt
     3121 atccggtccg ctgctcgccg accccggcgc ccgagccgtc ttcagccaga acggcgtgcc
     3181 gctgaccgag ggacagccgc tgcggcagcc cgatctcggc ggcactttgt cgcagcttcg
     3241 catcgccggg gtcggcgacc tgtaccaggg caccctggcg cgtcgcgtcg cgctggtttc
     3301 gcaggagatc ggcggcccga tccagctgtc tgatttgcgc ggggggctgc caaaactggc
     3361 agcgccgctg gttcgacagt acggcaatga ccgggttgct ttcctgccgc cgccggccga
     3421 tggcggcttg gccacggcag ccgcgttcga tgacctggca aacaacccga acgacaccgc
     3481 cggggccgtg gcccgttacc tgtcggtgtt ggcacgctat cgcgccgggg gcatttcggc
     3541 gaccgaagtc cttgcggcgc aaggcctgcc ggcggtcgga gccccgccgt ttcccgcctc
     3601 gaccagcctc gtggcgatgg accggtcggg aaacgccgtg gcgtgtgcgc taacaatgga
     3661 caatttgttc ggcaccggac ggatcatgcc cggcctgggc attctcgctg ccgcctcgcc
     3721 cgctgccgtg cagccaccgc tgctgtcggc gggaatggtc tggaatgaca acatccatgc
     3781 attccgtgct gcggccggcg gatccggtca ggccggtgcc ccgctggcgg tcgctttcgc
     3841 cctgatcaat acgctgaaaa ccgggcggcc actgagcacg ccggtgcctg atccgggacg
     3901 agccaacgtg attgcctgca gccggtatct gccaggggag accagcgagt gcggctgggc
     3961 caacgatcca cgcgaggccg gcttggcgat gggggcgaac tgatcgcggg cgtcatgcga
     4021 cttttggcca gcggctgcgc gctggtggcc gcggcgagcg cgcacgccgc ttttgccggc
     4081 gacacggctt ccggtgccac cagtatcggc ccgggccgga tcgtctgcaa cgccgcgttc
     4141 tgccaactcg gcagcggggc tcggccgaag gaacggttcc gggtcatcgt ttcggatctg
     4201 ccgcgggagg aaattcatcg cttacgaaag tgcaccggtg tggcgaagcc gtgcatcgtt
     4261 gcgatagacg gggtagagca gggcagcccg atgaggatca tggccaccag catccattgg
     4321 caggactgag gcgatggcaa taaagattgg caagggtgcc gcggcaccgc cattcctggt
     4381 gatggacgtc atcgcggcgg caaacgcccg gcaagccgcg ctggccgccg gtgcgccgca
     4441 tgtcctccgt atggaagtgg gccaacccgg caccggtgcg cccgccggtg ccgtcgccgc
     4501 tgctcaggcc gctcttcgtt ccggccatcc gctgggttac accgaagcgt tcggcctgcg
     4561 atcgctccgt caccggatcg ccgcccatgc cggggattgg tatggcctga agctgccgat
     4621 cgagcggatt gccgtcaccg ttggcgcatc cggagccttt ccgctggcat tcttggctgc
     4681 tttcgacccc ggcgaccgca tcgccctggc tgccccattc taccccccct acgtcaacat
     4741 cctgaccgcg ctcggcatga tccccgtgat cctggaagcc ggtctggaaa cccgcttcca
     4801 acccagcgtc gcgatgctgg aggcactgga gccgccgccg gatgggctga tcgtcgccag
     4861 cccctgcaat ccggccggga ctatgctgca tcgtgacgaa ctcgctgcca tcgcgacgtg
     4921 gtgcgaccgc aacggcgtgc ggctgatcag cgacgagatc taccacgggc tgaattacga
     4981 cagcccggtc gcctcagcgg ccgagttcag cgacagcgcg atcgtggtga acagtttttc
     5041 gaagtatttt tcgatgaccg gctggcggat cggctggctg gtgctgccgc cggacctgac
     5101 gcgtccggtg gagtgcctgg cgcagaacat gtttatcagc gcaccgtata tctcgcaggt
     5161 tgccgcggag gcggcgttcg actgccacga cgaactgcgg gcgaacgttg cgcgctatcg
     5221 gcagtcccgc gatcatttgc tggcggcgct gccggcctgc gggttcagcc ggctgtcacc
     5281 ggccgagggt gcgttctatc tgttcgccga catcagcgag cggtccaacg acagcgtggc
     5341 gttctgcgat cgaatgctgc gagaggccgg ggtggcggtt agtccgggtg tggatttcga
     5401 tcggacgcgg ggcaatcggt tcgttcggtt cagctattgc ggagcggaag aggatatgcg
     5461 ggcagcggcg gaacgtttgc gcgcctggcg gtaggccgat tcgagggcga gtaaacacaa
     5521 atgcttgaat agagtgagtc ggaacgtgta tcgagtttct ttgtccgcgg tttgtcgcgg
     5581 taaggaacga gatgagcgaa acgggggatc gcggcacagc aaattcggct ggttccagcg
     5641 acattttggg cagagccact cggacttcta ctctgggcgg atccaactcc gttcggctga
     5701 tagaggtgcc taagccgcct ttccctgcac caggatttca cgtcgagacg cgtgttgaaa
     5761 cacgctgcgt cgcgggcaga tcgccatcgt gacctggatc agcaatgcga aagcactcct
     5821 tgggcgctgg tggattatgc gtgaagcgcg cgcaatcgaa aggcggaaca acgaggaacc
     5881 ttactgggtc tttgagggta tgattaacga ggcccgggac gtgcttgata ggggcaatcg
     5941 agttcgtgca cgggaagtct gggttcaggc cagcgccatg ttcccagcca tggcaatgat
     6001 gtccacaaag gcactggact tccttctgcg gcttcaggcg tatgacgaag ctgatgcgct
     6061 ggtggcaaaa acccgaaagc aatacccgca tcgagcgcat tcgctagaag gattggcgaa
     6121 ggtggcctta aagcgcggct atcatgaaga ggcggtg
//
LOCUS       DASWQN010000306         4113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1218341, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000306 DASWQN010000000
VERSION     DASWQN010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218341"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1025..1597
                     /locus_tag="VGC82_05065"
     CDS             1025..1597
                     /locus_tag="VGC82_05065"
                     /inference="COORDINATES: protein motif:HMM:NF039976.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HEY0422675.1"
                     /translation="MMWTFDGAGDWFPLSSAVQFPGFRDHFDSVAPDEIKEFSPPFLG
                     ALQEPGLIQIWMGLVVRTAPGWSLLVRSPANLPRSGGWEAFEGIIETDRWFGPLITNL
                     RLTKTNVPIDFKPDYPLLQVQPIPRHVYEDATLNNYEIVPELAQLTPEDWDDYYDTVV
                     RPNSQVVRPRGQYAAMARKRRGAEGAADER"
     gene            1783..2853
                     /locus_tag="VGC82_05070"
     CDS             1783..2853
                     /locus_tag="VGC82_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEY0422676.1"
                     /translation="MRAWAVVESGQPLQEIELPTPEPTGTEVLLEVTHCGVCHSDLHI
                     WDGYYDVGGGQKMSLKDRGVVLPLAMGHEIVGRVVKLGPDASGVKPGDLRIVFPWLGC
                     GKCEKCLSEDDNMCTVAARSLGVFQNGGYGTHVVAPHPRHLVDPGHLDPAVAATYACS
                     GITVYSAIKKVMPLKPTQPIVLVGAGGLGLNAIAVLKALRHQNIISVDISAEKRDAAT
                     KAGATKTVDGSGDGAAVTKRIIEAAGGPVPAVIDLVNGTATARFAFGALDKGGKLIQV
                     GLFGGELTLALPIMAMRALTVQGSYVGNPKELRELVKLAQDGHLEALPVATVPQNQAN
                     DALMRLRDGKVTGRLILKAEAA"
     gene            2871..3905
                     /locus_tag="VGC82_05075"
     CDS             2871..3905
                     /locus_tag="VGC82_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY0422677.1"
                     /translation="MSSSGLRRSVVLKRRPRGEPTPDDFAIQEDAIPTAGPNQVVTRT
                     LWLSIDPYMRGRLREEQTYATAIQIGEVMTGETVGEVIASSNPDFVPGDIVAGARGWQ
                     SHSVSPGTQLFKIARGAAPLSAYLGVLGMPGATAYAGVTEICKPKAGETFVVAAASGA
                     VGSVAGQLAKRAGARVVGIAGGADKCLWVQDSLGFDDCVDHRSMDLHRELQAACPNGI
                     DCYFENVGGAVQAAVFDLLNPFARVAMCGMVSQYNEHTMPPGPNLGFVVGKRVLIQGF
                     IVSDRPARFTEWRAMAEPLVADGSLIYREDVIDGLENAPDALTGILSGRNFGKLLVRV
                     AAERPQASSH"
BASE COUNT          799 a         1276 c         1252 g          786 t
ORIGIN      
        1 cgtcacctga caggcgattt ttcaacgatc gcttagcggt gagataatgg aaaaatgcgg
       61 gattataacc gatttttttc gttattacga cgtgaattca ttgggacctg gcatttacac
      121 cttcgataac cgcggtggaa ccagacgaac gtggcgacga gctatatgaa gccttgactt
      181 gtggtaagcc agtggggtgc gcgtaaccca ctcggttcag ccaatggaag cacgaattat
      241 actaaggcaa aatgtactta tgataggaca atgctttaga ttccagctgc ggtagcttat
      301 gtttgacttc tggcaatcgt tctcagctgc ttaggcttgc gcccacctct ggagttctcc
      361 cagaactgtc ccagccgtaa ccatccgact gggtatcggt tgacatttga accgctgacc
      421 tgtcctcagg gaaattgccg taccgaacga gacgtggcgt ggctatttga ggacgcggcc
      481 tggagggggc tggaaccaat caaacgagcg acggcaaccg gcgcgctagc cactcacagt
      541 taatgcgaca tcgcccggcg aactttcaac gcaccgcttt ctgtccaaac tggttcggaa
      601 tcgtgccgta ttggcattgc tgcgaagccg tcatccccaa tatccggttc ctcagcgacc
      661 gtccaccttc gggaaagtcc gttgtgctga accgaaaaat aggcaccgca cgacaagcag
      721 cgcgccaagc ggtaaggttc agaccgacct tcagggtctg ttaactgctt gaaccgctca
      781 aggatccgtc gtgaatcaac ccaccccagc cgacgccgga agcaagctaa tcgtaaagcc
      841 gaagcttgtc acctttcacc aaatgattcc gactgctcgg gtgccgcagc gggctgaccg
      901 atccgccgcc ggttctctgc ccacacgagc gttccggtat tgtgagcctg ccacctctgc
      961 cgcggcgttt ggctactatc tgtttccgcc aatgggcttt tctgtgcagt gggacggata
     1021 cgacatgatg tggacgttcg acggagcggg cgattggttc ccactcagtt ctgctgtgca
     1081 gtttcccggc ttccgtgacc acttcgactc ggtagcaccg gatgagatca aggaattttc
     1141 cccacccttc ctcggcgctc tgcaggagcc cggcctgatc cagatttgga tggggctcgt
     1201 tgtgcggacg gccccaggtt ggagcttgct ggttcgctcg cctgctaacc tcccgcgcag
     1261 cggcggctgg gaagcctttg agggaattat cgaaaccgat cgctggttcg gcccgctgat
     1321 caccaacctg cgactgacca aaaccaacgt tccgatcgac ttcaagcccg actacccgct
     1381 acttcaggtg caaccgatcc cgcggcatgt ttacgaagat gcaaccctga acaactatga
     1441 aattgtgccc gagctagcgc aactcacacc cgaggactgg gacgactatt acgacacggt
     1501 cgtccgcccc aactctcagg tcgtccggcc tcgcggccag tacgcggcta tggcccgcaa
     1561 acgccgcggg gccgaaggcg cagcggatga aaggtagatt gagaatccgg gtcagatcgt
     1621 agcttccgca ggcgatgctc catcggttcg ggatggaaga atccggaccg gacattgcca
     1681 acctactcat cggccgggca tggccggggc gtaagcccgt ctcttgcgca atccgcgcca
     1741 cgcgctatcc atccgtcgaa cgccaaacag caggagaccg acatgcgcgc atgggccgtc
     1801 gtcgaaagcg ggcaaccgct tcaggaaatc gaattgccga cacctgaacc aaccggcacc
     1861 gaggttctgc tcgaggtcac gcactgcggc gtctgtcatt ccgatctgca tatctgggac
     1921 ggctactacg acgttggcgg cggccagaag atgtcgctga aggaccgcgg cgtcgtcctc
     1981 ccgctcgcga tgggccacga aatcgtcggc cgcgtcgtca agctcggtcc cgacgcctcg
     2041 ggggtaaaac cgggcgacct gcgcatcgtt ttcccgtggc tgggctgcgg caaatgcgag
     2101 aaatgcctgt ccgaagacga caacatgtgc accgtcgccg cccgcagcct gggcgtgttc
     2161 cagaacggcg gctatggcac tcatgtcgtg gccccgcatc cgcgccacct cgtggatccc
     2221 ggccatctgg atccggcagt tgcggcgact tacgcgtgct ccggcatcac ggtgtattcc
     2281 gcgatcaaga aagtcatgcc gctgaagccg acgcagccga tcgtgctggt gggggcaggg
     2341 ggccttggcc tgaatgctat cgcggtgctg aaggcgctgc ggcatcagaa catcatcagc
     2401 gtggatatca gcgcggaaaa acgcgacgcc gccaccaagg ccggcgcgac caaaaccgtt
     2461 gatggcagcg gcgacggcgc ggcggtcacc aaacgcatca tcgaggctgc cggcggcccg
     2521 gttcccgcgg tgatcgacct ggtcaacggc accgccaccg cccgcttcgc ctttggagcg
     2581 ctggacaagg gcggcaagct aatccaggtc ggcctgttcg gcggcgaact gacgctggcg
     2641 ctgccgatca tggcgatgcg cgcgctgacc gttcagggca gctatgtcgg caatcccaag
     2701 gaactgcgcg aactggtcaa gctggcgcag gacggccatt tggaagccct cccggtcgcc
     2761 accgttccgc aaaaccaggc caacgacgcc ctgatgcggc tgcgcgatgg caaggtcacc
     2821 ggacgcctga tcctgaaagc cgaggcggca tgatgagcag ttcaagtcct atgagcagtt
     2881 caggccttcg ccgttcggtc gtgctcaaaa ggcgtccccg cggtgaaccc actcccgacg
     2941 atttcgccat ccaggaggac gcgatcccaa ccgccggtcc gaatcaggtt gttacccgaa
     3001 ccctctggct gtcgatcgat ccgtatatgc gcggacgcct gcgcgaggag caaacctacg
     3061 ccaccgcgat ccagatcggc gaggtcatga ccggggaaac cgtgggcgag gtcatcgcct
     3121 cctctaaccc cgatttcgtg ccgggcgaca tcgttgccgg cgcgcgcggc tggcagagcc
     3181 attcggtcag cccgggaacg cagctgttca agatcgccag gggagccgca ccgctatcgg
     3241 cgtatcttgg ggtgctcggc atgccaggcg ccacggccta cgctggcgtg acggaaattt
     3301 gcaagcccaa agccggcgaa acgttcgtgg tggctgccgc atccggtgcg gtcggttcgg
     3361 tggccggtca actcgccaag cgggcaggcg cgcgcgtggt cgggatcgcc ggtggcgccg
     3421 acaaatgtct ttgggtgcag gacagcctgg ggtttgacga ctgcgtggac caccgctcga
     3481 tggacctgca tcgggagttg caggcggctt gcccgaacgg catcgactgc tacttcgaaa
     3541 acgtcggcgg cgcggtgcag gcggcggtgt tcgatttgct caacccgttc gcccgcgtgg
     3601 cgatgtgcgg catggtttcg cagtataatg aacacaccat gccgcccggg ccgaacctgg
     3661 gcttcgtggt cggcaagcga gtgctgatcc agggcttcat cgtctccgac cggccggcac
     3721 ggttcaccga gtggcgtgcg atggcggaac ccttggtcgc cgatggcagc ctgatttacc
     3781 gggaggatgt gatcgacgga ctggagaatg cgcccgatgc gctgaccggc atcctgagcg
     3841 gccgtaactt tggcaagcta ctcgtcaggg tggcggcgga gcgccctcag gccagctcgc
     3901 attgaaacca gctcaaggcg gaccaccctg gcgtcatcag gaccgcgaaa tcgcctagtg
     3961 tcccggatct gaagtccgcc atatccggcg gacctcagtc tcgcgccacc ctctttttgt
     4021 ttcattggcc tgctgatccc cctgcattcg tcgcatgatg cgacgaactt cgggggcagg
     4081 gccctagact attgtcatcc tgactgtgca ggc
//
LOCUS       DASWQN010000307         5644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_822833, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000307 DASWQN010000000
VERSION     DASWQN010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822833"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VGC82_05080"
     CDS             complement(<1..1250)
                     /locus_tag="VGC82_05080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0422678.1"
                     /translation="MSPPVDPVASDPTLPSRTDVVVIGGGIVGVSTALFLAEKGIAVT
                     LCEKGCIGGEQSSRNWGWCRTMGRDVGEIPLAMESLRLWRGMNQRTGRETGFRQAGVM
                     YLCQKDADIAAQQAWLEQASQYQVDARLLKGAALEATMPGATNGFIAGLHTPSDGKAE
                     PALAAPAIAEAARDHGALIFANTAVRGIDQTAGRISGVVTERGRIACQSVVLAAGVWS
                     RLFAGNAGLDLPQLKVLGSVFRTKPLKGPEITAAGGVFAFRKRMDGGYSIARRNANTV
                     ELTPDHFRLLSDFLPRLINNRNEIRLRVGTRSWEELRTARRWSLDKVTPFEAVRILDP
                     KPKEGILREARSFLSRAFPVFAGMEVVESWAGLMDVTPDAVPVIDQVDHMPGFFIATG
                     FSGHGFGIGPGAGRLMADMVAGDRT"
     gene            1383..2114
                     /locus_tag="VGC82_05085"
     CDS             1383..2114
                     /locus_tag="VGC82_05085"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0422679.1"
                     /translation="MSRLPNKIILITGAARGQGAAEARRFVREGARVVITDILEAQGQ
                     ELARELGKDAVFLRHDVSSETDWQNAVEAASAMGGLHGLINNAGIYRPGTLMETDAAL
                     FEQHMRINQLGPFLGMKAVVPLMERSGGGAIVNISSVAGLRGSPGAIAYSATKWALRG
                     MTKAAAVDLAPRNIRVNSVHPGPIDTPMLDVRTPEQNRQRLQSVPMKRRGTPEEIADL
                     VLFLISDESRYITGAEIAIDGGATL"
     gene            2891..3973
                     /locus_tag="VGC82_05090"
     CDS             2891..3973
                     /locus_tag="VGC82_05090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010505462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0422680.1"
                     /translation="MTVEFIGMIHHREASEIIAPPPVILDRGYIRDFAQAAEAGGFDK
                     VLVGYFSDGPDGFLIAAAAAAHTEILGFLVAHRPGFVAPTLAARKFATLDQITGGRAA
                     IHVISGGDDTDQFRDGDTLAKEDRYARTDEYVSVLKRIWTEPSPTVDHEGRFYRFAGA
                     STAQRCVQQPRIPIYFGGASTAAITVSAKHADVYALWGETHDQVREITARLRAEAAKH
                     DRTVRVSLSLRPILAPTEDEAWARAEAIKQRIIERRAAAGLGPRNPVNEGSRRLLAAA
                     AKGERLDKRLWTGAALLTGASGNTTSLVGTPAQVAEALCEYYDLGVTTFLIRGFNPLD
                     DTIAYGRDLLPLTRQMIAERTAARAA"
     gene            complement(4013..5155)
                     /locus_tag="VGC82_05095"
     CDS             complement(4013..5155)
                     /locus_tag="VGC82_05095"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0422681.1"
                     /translation="MSLSLDGVRVLELARYQAGPRGGMILSDLGAEVIKIERIGGEET
                     RKNAPVVRGQSIYFTVYNRGKKSICLDMRSAEGKAIFTDLVKQADVVLENFRPGTMAA
                     MGFGYDALKAINSGIILTSVSGFGQYGPYTERPAFDPLGQAMSGLMDLTGKPVGQPLG
                     VASSVVDRYTALHATIGTLAALRHRDRTGEGQMVDVCLMDSALTMVEIPASYYLSTGE
                     EGGEAGRPPYRAKDGYVVISAAGREMAARLMTLVSGAGADTSPLVPAATDARRAALEA
                     WCAARSVNEIVSTLLDLGVPVAPVHTIPQVVRDPHVWAREMLVKIDDPIAGEVYVPGV
                     TVKMSHTPGRIGPVPTPGQHTDEVLGGLLGFDAERLRTLREAKVIG"
BASE COUNT         1058 a         1851 c         1743 g          992 t
ORIGIN      
        1 gtgcggtctc cggccaccat gtcggccatc aggcgcccgg cgccgggccc gatgccgaag
       61 ccgtggcccg agaagccggt cgcaatgaag aagccaggca tatgatcgac ctggtcgatc
      121 accgggacag catcgggggt gacatccata agacccgccc agctttccac cacctccatc
      181 ccggcaaaaa ccggaaaggc tcgcgacaga aacgagcggg cttcccgcag gattccttcc
      241 ttgggttttg gatcgaggat gcggaccgcc tcgaacggag tgaccttgtc cagcgaccag
      301 cggcgggcgg tgcgcagttc ttcccaggaa cgagtgccga cgcgcagccg gatctcgttg
      361 cggttgttga tcaggcgcgg caggaaatcc gacagcagcc ggaaatggtc gggcgtcaac
      421 tcgaccgtgt tggcgttgcg gcgggcgatc gaatagccgc cgtccatccg cttgcgaaaa
      481 gcgaacaccc cgccggcagc ggtgatctca ggccccttca gaggcttggt gcggaacacg
      541 gaccccagca ccttgagttg cggcagatca aggccggcgt tgccggcgaa caggcgggac
      601 cagacgcctg ccgccaggac gacagactgg caagcgatgc ggccgcgttc ggtgacgacg
      661 ccgctgatcc ggccggcggt ttgatcgatg ccgcgaacgg cggtgttggc gaagatcagc
      721 gcaccgtggt cgcgcgccgc ctcggcgatg gcgggggcag cgagggcggg ttcagccttg
      781 ccgtccgagg gcgtgtgcag tccggcgatg aagccgttgg tggcgccggg catcgtggcc
      841 tccagcgcgg cacctttcag caggcgcgca tcgacctgat attggctggc ctgttcgagc
      901 caagcctgct gggcagcaat gtcggcatcc ttctggcaca ggtacatgac cccggcctgg
      961 cgaaatccgg tttcacggcc ggtgcgctgg ttcatgcctc gccaaagcct caggctttcc
     1021 atagcgagcg ggatttcgcc cacatcgcgt cccatggtgc ggcaccagcc ccagttgcgt
     1081 gaagactgct cgccgccgat acagcccttc tcgcacagcg tgacggcgat gcccttttcc
     1141 gccaggaaca aggcggtcga gacgccaacg atgccgccgc cgatgacgac cacgtcggtt
     1201 ctcgagggaa gcgtgggatc ggacgcgaca ggatcgacgg gtggagacat gcgatggcgt
     1261 ggtccatgcg gcgatcggca gcttaggatc gcggttcggg ttttggctac gggtgtttcc
     1321 gaccggattt gacaaccccc ggttcgccgc cctctctttc tgccaaacaa gaagggaatt
     1381 ctatgtccag gctgccaaac aaaattatcc tgatcaccgg cgcggctcgc ggccaaggtg
     1441 cggccgaggc acggcggttt gttcgtgaag gcgcccgtgt cgtcatcact gatatccttg
     1501 aagcgcaagg tcaggaactt gcccgggaac tgggcaagga cgcggttttt ctgcgccacg
     1561 acgtttccag cgaaacggat tggcaaaatg ccgtcgaggc cgcttccgcc atgggcggcc
     1621 tgcacggcct gatcaacaat gccggcatct accgacccgg caccctgatg gagactgacg
     1681 ctgccctctt cgagcagcat atgcgcatca accagctcgg ccccttcctc ggcatgaagg
     1741 ccgtcgtccc tttgatggaa cgctcgggtg gaggcgcgat cgtcaacatt tcctccgttg
     1801 ccggcctgcg cggttccccc ggtgctattg cctatagcgc caccaaatgg gctctgcggg
     1861 gcatgaccaa ggccgctgcg gtcgatctcg cgccacgcaa cattcgggtg aactccgtcc
     1921 accccggccc catcgacacg ccaatgctgg acgtccgcac gccggagcag aaccgccagc
     1981 gcctgcagtc cgttccaatg aagcgcaggg gaaccccgga agaaatcgcc gacctcgtgc
     2041 tgttcctgat ttcggatgaa agccgctaca tcaccggcgc cgaaatcgcc atcgacggcg
     2101 gcgcgacgct gtaatcggtg cagtgccgat acgtccgttg cgcaccagcc atgtgccgga
     2161 cttcaactgg atttacttct ctgttacatt gcggtcatgg ttccttggcg caccaattgg
     2221 attttgatct tcccgcggat gccgcgcttg cgacacagct tgaggccgaa gtcggtttct
     2281 cagcaggtcg agtcgtcccg cacaagactg tccgggagcg gctggcgaag ctggcgagag
     2341 agttccgccg ccatccagct gaatgcgcgt tggttggacg gctgcggccc tacgcggagt
     2401 gatgcacgcg tataactccc tcgccgaatc cagcccacac gctgctcggc atgtcgccgc
     2461 aactctgatg gaggcaggcg atagcctcaa gaattcccca catcgcggcg gacgtgtgac
     2521 tgcgtcccag agcgccgggg caaggctcgg tcgcttcgtt cgctggatcg atgcattaga
     2581 gtagatcccg ttcagataga ttatctgagc gggtgaagat gctcgctaaa acaacgagct
     2641 agaccgtttt cagtgagccc aggcgaacgg aaaacggtcc agagcggttt cacggtaact
     2701 ggaaacgaag ctctcttgtg gccgcaaaga aactcgtggg tggccgtgcc aagcatggcc
     2761 atgacactgg gtggccatgc caagcacgac cataacacat cgacgccaag cggccatctg
     2821 actcgcacct tttccagcca acgtgaagcg ttctagcctg ccgccagcag atggaaacaa
     2881 ggaagcaccc atgaccgtcg aattcatcgg catgatccac caccgcgagg catcggagat
     2941 catcgcaccg ccgccggtga tcctggatcg cggctacatc cgcgacttcg cccaagccgc
     3001 cgaagccggt ggcttcgaca aggtccttgt tggctacttc tccgatgggc cggacggctt
     3061 cctgattgcc gccgcggccg ccgcgcacac ggaaattctg ggtttcctcg tcgcccatcg
     3121 ccctggcttc gttgccccta cgctagcagc ccgcaagttc gccacgttgg accagatcac
     3181 tggcggacgg gcagcgatcc acgtcatcag cggtggcgac gacaccgacc agttccgcga
     3241 cggcgacacg ctggcgaagg aagaccgtta cgcccgtacc gacgaatacg tgtcggtcct
     3301 gaagcgtatc tggacggagc ccagccccac cgttgatcac gaaggccggt tctatcggtt
     3361 cgccggcgcc tccaccgcgc agcgctgcgt gcagcagccg cgcatcccga tctactttgg
     3421 cggcgcctcc accgctgcga tcaccgtcag cgccaaacat gccgacgtgt atgcgttgtg
     3481 gggcgagaca cacgaccagg tgcgggagat caccgcccgg ttgcgcgccg aggccgcgaa
     3541 gcacgatcgc accgtccgcg tcagcctgtc gctgcgcccg atcctggcgc cgaccgagga
     3601 cgaggcatgg gcgcgcgccg aggccatcaa gcaacggatc atcgaacgcc gcgcggcagc
     3661 cggtctcggt ccacgcaatc cagtgaacga gggatccaga agacttctcg ctgccgcggc
     3721 aaagggggag cgtctggaca agcgcctatg gaccggcgcc gcgctgctga ccggggccag
     3781 cggcaacacc accagcctgg tcggcacgcc ggcccaggtc gccgaggcat tgtgcgagta
     3841 ctacgatctt ggcgtgacga ccttccttat tcgcggcttc aatccgctgg atgacaccat
     3901 tgcgtacggc cgggatttac tgcctttgac ccggcagatg attgccgaaa gaaccgctgc
     3961 gcgagcggcc tgatcagcga aaccaacccc ggtggaacca accccggtca cctcacccga
     4021 ttacctttgc ctcccgcaac gtccgcaggc gctcggcgtc aaaccccagt aacccgccca
     4081 gcacctcatc cgtatgctgt ccgggcgttg gcaccggtcc gatccggccg ggcgtatgcg
     4141 acatcttgac cgtcacgccg ggcacgtaaa cttcgcccgc tatcggatcg tcgatcttca
     4201 ccagcatctc acgcgcccat acgtgcgggt cccttacgac ttgcggaatg gtatgaaccg
     4261 gagccaccgg cacgcccagg tcaagcaacg tcgatacgat ctcgttcacc gagcgtgcgg
     4321 cgcaccaggc ctccagcgcg gcgcggcgcg catcggtggc ggcgggaacg agcgggctgg
     4381 tgtcggcccc ggcacccgag acgagcgtca tcaaccgagc cgccatttct cgtccggcgg
     4441 ccgagatcac gacgtagccg tccttggcgc ggtagggcgg acggcccgct tcgccgccct
     4501 cctctccggt cgacagataa tacgaggccg gaatctccac catcgtcagc gcggaatcca
     4561 tcaggcagac atcgaccatc tgcccctcgc cggtgcggtc gcggtgccgc agcgccgcca
     4621 gcgtgccgat ggtcgcgtgc agcgcggtgt agcgatcgac gacggaggac gcgacgccca
     4681 gcggctgccc gaccggcttg ccggtcaggt ccatcagccc gctcattgcc tggcccagcg
     4741 gatcgaaggc agggcgttcg gtgtacggac cgtactggcc gaaccccgat accgatgtca
     4801 ggatgatgcc gctgttgatg gctttcagcg cgtcatagcc gaaccccatc gccgccattg
     4861 tgccgggccg gaagttctcc agcaccacgt cggcctgctt caccaaatcg gtgaagatcg
     4921 ccttgccttc ggcactgcgc atatcgagac aaatgctttt cttgccgcgg ttgtagacgg
     4981 tgaaatagat gctctgcccg cgcacgacgg gggcattctt gcgcgtctcc tcgccgccga
     5041 tgcgctcgat cttgatgacc tcggcgccga gatcggacag gatcatcccg ccacgcggac
     5101 ccgcctggta gcgcgccagt tccagcacgc gcacgccgtc gagactgagg ctcatggttt
     5161 cctcccggtt tcaggtcatg aaagaccgga cggcagaagc gggcaagcca ggcaaatccg
     5221 aagtccaccg aagtgtgcgg ctgtgaggct gaaaccgtcc ttctcgtcgg tgatccacct
     5281 taaccagcac agtgatcgtg gcgccgcgag aggaaagagc gttgccttgc gtgagcgccg
     5341 catttcgaag ccacccgaca cagctctgaa agatatgctc ggtccacgcg agaacccgga
     5401 gcgctcgatt ccagcgttga ggccgtttat ccgctgcgaa ttctcacgtg gacgggtata
     5461 ttccatgctc acgtttttgt gatattagca cgggaatatt ctcacggctt ggacggtatc
     5521 cttgcagcct gacccagtaa ttggcccaat gaaacgggga atatatgcgg cggttcaccc
     5581 acctgatcac agggcgacat catgcaaagc aatggggcgg tccagatccg ccgcttcaat
     5641 gtcg
//
LOCUS       DASWQN010000308         3097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2009926, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000308 DASWQN010000000
VERSION     DASWQN010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2009926"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VGC82_05100"
     CDS             complement(<1..722)
                     /locus_tag="VGC82_05100"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEY0422682.1"
                     /translation="MAASLSSHDVARLLSEPSSDMRADLASKVAADLTGNALTPAEVK
                     MAQDIVRILARDLEEKVRASLSRGLRHAANLPRDVALKLARDVEYVALPMLADSLVLT
                     DDDLIEIVRNGSSLKQEAIASRQNLPLTVSDALISHADEPAVVVLMGNNTAAIGADGF
                     NRAITRFAASDRVTQAMVLREKLPATVAERLVTLVSRALQAHLVTAHELAPATAADIV
                     LTSREHAIIHLSLGASDEDLRQ"
     gene            complement(854..2863)
                     /locus_tag="VGC82_05105"
     CDS             complement(854..2863)
                     /locus_tag="VGC82_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEY0422683.1"
                     /translation="MKWLRMLMLGLMAPVMVPTMPAHALESAPIASKRAVATLVTDTD
                     AVQPGKPFRVGLRLRMADGWHTYWKNPGDAGVPPELTIEGGSQSAIDWPTPRRVAEGP
                     VMTYAYTGEVLLPVTVTAATGPIRAHAQWLVCKDICVPEEGDFSLTLPAGIPLASAQA
                     DLFAAHDRAVPRPSPWAARISPDGILFVQGPELKAETVTDAWFIPDQPGRIQDDAAQP
                     LSVREGGFALELKPAKEFASAKDLSGVLSVRDRSGLQTDVVVNAVPGAAPDPAMPPLG
                     QILVFAFLGGLILNLMPCVFPILAMKAVALARHAGRGSAHAMSYTAGVLVTFMALAGA
                     LLAARAAGTAAGWGFQFASPIFVAAMTWLLFAVGLNLSGVFQVGAGLTGAGSGLARHN
                     GTVGSFFTGLLAVLVATPCTAPFMGLAIAAGLAAPPVVTLLVFAVMGLGLAAPYVALA
                     SMPGLARLMPRPGRWMELLKQGLAFPMYAASAWLVWVVSQEAGPSGVLGASVGLVLIG
                     FAGWVFGVTQTSAVQPRRFGQAAAMVAVLAALAVLSGISASPVGAASDKSVEAYTPER
                     LAALRTQGRPVFVNMTAAWCVTCLVNERVAISTDAVQAAFAAGGVTYLKGDWTRQDPA
                     ITAFLRQHGRDGVPLYVFFPASGEQPRVLPQILTEGEVLRQLRMS"
BASE COUNT          567 a         1106 c          946 g          478 t
ORIGIN      
        1 atctgccgca ggtcttcatc cgaagcgcca agactgaggt ggatgatcgc atgctcgcgc
       61 gacgtcagca cgatgtcggc ggccgtggcc ggtgccagct cgtgcgctgt caccaaatgc
      121 gcctgcaacg ccctcgagac cagggtcacc aaccgttcgg ccacagtggc cggcaatttt
      181 tcccgcagca ccatcgcctg cgtgacgcga tcgctggccg cgaaccgggt tatcgcccgg
      241 ttgaagccgt ccgccccgat ggccgccgta ttgttgccca tcagcacgac caccgcgggc
      301 tcgtcggcat ggctgatcag cgcgtcgctc accgttagcg gcagattctg acggctcgcg
      361 atcgcctcct gcttcagcga cgagccattc cggacgattt cgatcaagtc gtcatccgtc
      421 agcaccagcg aatcggccag cattggcaaa gcgacgtatt cgacatcccg ggcaagcttg
      481 agcgcgacat cccgcggcag gtttgccgca tgtcgcagtc cgcgcgacag cgaggcacgg
      541 accttttcct ccaggtcccg tgccaggatg cggacgatat cctgcgccat tttcacctcg
      601 gccggggtga gcgcattgcc cgtcaggtcc gctgccacct tgcttgcaag gtctgcgcgc
      661 atatcggagg agggttccga caacagccga gcgacatcgt gggacgagag agacgcagcc
      721 atggggaacc ccgcgtacgt gatcgaaccg cgggagtgtg ccagcgatag ataaacaacc
      781 ggttaacgac tcggcgcgcc cgatccgcgc ttcgacgggc cgatccattg ggcgttcgga
      841 agcgaggcat tggctacgac atccgtaact gtcgcagcac ttcgccctcc gtcaatatct
      901 gaggcaacac ccgcggttgc tcgccagagg ccggaaaaaa cacgtaaagt ggaaccccgt
      961 cgcggccgtg ctgccgcagg aacgcggtga tcgccgggtc ctgccgggtc caatccccct
     1021 tcaggtatgt cacgccaccc gccgcgaacg cggcctgcac cgcgtcggtg gagattgcca
     1081 cgcgctcgtt caccaggcag gtcacgcacc acgccgctgt catgttcacg aataccggcc
     1141 ggccctgggt gcggagcgct gcaagccgct ccggcgtata ggcctcgacc gacttgtcgc
     1201 ttgccgcccc aaccgggctc gcgctgatcc cggacaggac cgccaatgcc gccagcaccg
     1261 cgaccatcgc cgcggcctgt ccgaaccggc gcggctgcac cgcggaagtc tgcgttaccc
     1321 caaacaccca tccggcaaac ccgatcagta ccaacccgac tgacgccccc agcacgccgg
     1381 aaggccctgc ctcctgactg acgacccaga ccagccaggc agaggccgca tacatcggaa
     1441 aggccaggcc ctgcttcagc agttccatcc accggcccgg gcgcggcatc agtcgagcca
     1501 gtccgggcat cgaggcgagg gcgacatagg gtgcggccaa ccccagcccc atgaccgcga
     1561 acaccagcag ggtcacgacg ggaggtgccg ccaggcctgc cgcgatcgcg agccccatga
     1621 acggcgcggt gcacggcgtc gccaccagca ccgccagcag accggtgaag aagctgccta
     1681 cggttccgtt gtgcctggcc agtccgcttc cggcaccggt cagcccggcc ccgacctgga
     1741 acacgccgga caggttcagc ccgaccgcga acagcagcca ggtcattgcc gcgacgaata
     1801 tcggcgaagc gaactggaat ccccagcccg ccgccgtccc ggctgccctt gccgccagca
     1861 gtgcgccggc cagggccata aacgtcacca ggacaccggc cgtgtaggac atcgcatgag
     1921 cactgcctcg gccggcatga cgtgccagcg cgaccgcctt catcgcgagg atcgggaaca
     1981 cgcacggcat caggttcagg atcaggccac ccaggaaggc aaaaaccagg atctgtccca
     2041 ggggcggcat ggctgggtcg ggcgccgcgc ctgggaccgc gttcaccacc acgtccgtct
     2101 gcaggcccga ccggtcgcgc accgacagca ccccggacag atccttcgcc gacgcgaatt
     2161 cctttgccgg cttcaactcc agggcaaagc cgccttcccg caccgacagc ggctgcgccg
     2221 catcgtcctg aatccggccg ggctggtcgg gaataaacca ggcatccgtg accgtctccg
     2281 ccttcagttc cgggccctgc acgaacaaaa tgccatcggg cgatatccgc gccgcccagg
     2341 gcgacgggcg tggcaccgcc cggtcatgtg ccgcgaacag atccgcctga gccgaggcaa
     2401 gcggtatccc ggccggcaag gtcagggaaa aatcgccttc ttccggcacg cagatatcct
     2461 tgcacaccag ccactgcgca tgggcgcgga ttgggccggt cgccgcggtt accgtgaccg
     2521 gcagcaacac ctcgccggtg taggcatagg tcatcaccgg tccctcggcg acgcgccgcg
     2581 gcgtcggcca gtcgatggct gactgggagc cgccctcgat cgtcagctcc gggggcaccc
     2641 cggcatcccc cgggttcttc cagtaggtgt gccagccgtc cgccatgcgc agccgcagcc
     2701 ccacccggaa cggtttgcct ggttgcacgg catcggtgtc ggtcaccagc gtcgcgaccg
     2761 cccgcttgct ggcgattggc gcactttcga gcgcatgggc cggcatcgtc ggaaccatca
     2821 ccggcgccat caggccgagc atcaacattc gaagccactt cataccgtct ccgcctgccg
     2881 ccaatggcgc tgattcgcgc ttgttacgcc gtcgatgcca accaaggggg ttaccttagc
     2941 ggatttttcg tcctagcgtt cacataatgc gacgaacggc gacaccgatt tattgtggga
     3001 gaaaacgcac atgagtacca gtggcttcgg cagtaccagc ggcttcggcg gagactgcct
     3061 cgccggcgtg cgtgtcctgg atatgaccca attcgag
//
LOCUS       DASWQN010000309         3356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_558503, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000309 DASWQN010000000
VERSION     DASWQN010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558503"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VGC82_05110"
     CDS             complement(<1..374)
                     /locus_tag="VGC82_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY0422684.1"
                     /translation="MAVTTRIDDPVLIRFCKALGEIYGNQIDCVVLFGSRARGDARPD
                     SDYDVAVFLKSLPDLLEVLARSGGQGGGVGSFGYPPRQHERRNRRLLRRHTLCSRPIW
                     RAFAVFARNSQRWTCVLTPEAAR"
     gene            complement(459..1658)
                     /locus_tag="VGC82_05115"
     CDS             complement(459..1658)
                     /locus_tag="VGC82_05115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0422685.1"
                     /translation="MAHRGLTFGIFLAPFHRLGENPTLAIDRDVELIQWLDYLGYDEA
                     WIGEHHSAGWELIASPELIIAAAAEKTKHIKLGSGVTSLPYHHPFLVAQRWVQLDHMT
                     RGRAMLGCGPGALTSDAYMLGIQPSTQRPRMLQSLDAIMQLLKMEERVTIKTDWFEMN
                     DARLHLAPYSDPHFEIAVASTITPFGMIAAGTHGVGVLSIGAGLPGGPEALASQWKIA
                     EDTAAKNGKTMSRKKWRVVVNAHLAEDDEQALNEVRKGERNETITYFEDTLGRPPGRS
                     DDPLSDGVKMGTTLVGSPETVTKGIKRLLGYSQGGFGGILFRAHEWATREQTMRSYEL
                     FARYVMPVFQQSLEAPVGSRTWAQENRKQVFGSTPDAVRRAFTDFGAAVPEDFKQRTL
                     GGRDVTE"
     gene            1769..2521
                     /locus_tag="VGC82_05120"
     CDS             1769..2521
                     /locus_tag="VGC82_05120"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0422686.1"
                     /translation="MRLAGKIAIITGAASGMGAATARRFGQEGARVVIADMLEEEGKA
                     VADSINAAGGKAVFMALNVTEEAGWKKVVDQTVAAFGQLDILVNNAGISGSATNDLLD
                     SALWERVMAVNSTGVFLGTAAAVRQMQRNGGGSIVNLSSISGVVGQTMVHMGYNAAKG
                     AVRIMTKSTAVQFGKDRIRCNSVHPGLMPPMRTSGATADPAVRARMLKAVPLGRTGEV
                     DEVANAILFLASDEASYITGAELYVDGGYLAS"
     gene            2658..3074
                     /locus_tag="VGC82_05125"
     CDS             2658..3074
                     /locus_tag="VGC82_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0422687.1"
                     /translation="MKILRSSARKTNRAPASNFTGTVFSDEVVVGTAPSRMRGSVVSF
                     TPGARTAWHSHPVGQTLFCLSGAGRVQREGEQVQEIRPGDTVVIPPDVRHWHGSAPDK
                     LFSHLAMSELNDKGEGTAWFEHVSDEDYNAPPAPMT"
     gene            complement(3107..>3356)
                     /locus_tag="VGC82_05130"
     CDS             complement(3107..>3356)
                     /locus_tag="VGC82_05130"
                     /inference="COORDINATES: protein motif:HMM:NF018599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEY0422688.1"
                     /translation="MLELRPNCECCDRDLPPDSQEARICTYECTFCADCVEHRLHGIC
                     PNCGGEFVRRPVRPAHMLAKHPASTKRVLKPEGCGAAA"
BASE COUNT          619 a         1116 c         1028 g          593 t
ORIGIN      
        1 cgagctgcct cgggcgtcaa aacgcaggtc catcgttgcg aatttcgtgc aaaaacggcg
       61 aacgctctcc atatggggcg gctgcagagg gtgtgacgcc gaagaaggcg ccggtttcgt
      121 cgctcatgtt gacgcggagg atagccaaac gatccaactc cgcctccctg gccgcccgat
      181 cgggcaagga cttcaagaag atcgggcaag gacttcaaga agacagcaac atcgtagtcg
      241 gagtcaggac gcgcatcacc ccgcgcccgt gagccgaaca gcaccacaca gtcgatctgg
      301 ttgccataga tctcgccaag cgctttgcaa aagcgaatga gcacggggtc gtcgatccgt
      361 gtcgtcactg ccatcgccgc atcgccatcg cgctccttcc cagtgaggcg aaagcggaat
      421 tcactttcag cccgctaagc gtgcccgcgt agcccatgtc actccgtcac atcccgacca
      481 cccagcgtcc gttgcttgaa gtcctcgggc accgccgcgc caaaatcggt aaacgcccgc
      541 cgcaccgcgt ccggcgtgga accaaacacc tgcttgcgat tttcctgcgc ccacgtcctc
      601 gatcccaccg gagcctccag cgactgctga aacaccggca tcacataccg ggcgaacagc
      661 tcgtagctgc gcatcgtctg ctcgcgcgtc gcccattcat gggcacgaaa caggatcccg
      721 ccgaacccgc cctggctgta tcccagcagc cgcttgatcc ccttggtcac tgtttccggc
      781 gaacccacca acgtcgtccc cattttcacg ccgtcgctca atgggtcgtc ggagcggccc
      841 ggcggccggc ccagcgtgtc ctcgaaatag gtgatggtct cgttgcgctc gcccttgcgg
      901 acctcgttca acgcctgctc gtcgtcctcg gcgaggtgcg cgttgaccac cacgcgccat
      961 ttcttgcggc tcatggtctt gccgtttttc gcggcggtgt cttcggcgat cttccactgg
     1021 ctggccaaag cctccgggcc gcctggcagg ccggcgccga tcgacagcac gccgacgccg
     1081 tgcgtcccgg cggcgatcat gccgaacggg gtgatggtgc tggcaaccgc gatctcgaaa
     1141 tgcgggtcgc tgtagggtgc caggtgcaga cgcgcgtcgt tcatctcgaa ccagtcggtc
     1201 ttgatcgtca cccgctcctc catcttcagc aactgcatga tcgcgtccag cgactgcagc
     1261 atgcgcggcc gctgcgtgga cggctgaatc cccagcatat aggcgtccga ggtcagcgcg
     1321 ccggggccgc agcccagcat tgcccgccca cgggtcatgt gatcaagctg cacccagcgc
     1381 tgcgccacca ggaacggatg gtggtagggc aggctggtga cgccggagcc cagcttgatg
     1441 tgcttcgtct tctccgccgc cgcggcgatg atcagttcgg gggaggcgat cagttcccag
     1501 ccggcggaat ggtgctcgcc gatccacgcc tcatcatacc caaggtaatc cagccactgg
     1561 atcagttcca cgtcgcgatc gattgccagc gtcgggtttt cacccagcct gtggaacggc
     1621 gccaggaaga tgccgaaggt caagccgcgg tgggccataa gcagtgctcc agttcgtgaa
     1681 ccgcctccat cgtgcctcag ataccagccg gtagccaaga gtcggcggcg atggcaggat
     1741 tggatcaaca aaacggaggg gaacaatcat gcgcctggca ggcaagatcg ccatcattac
     1801 cggtgcggca tccggcatgg gcgccgccac ggctcgtcgg ttcggccagg agggggcacg
     1861 cgtcgtcatc gccgacatgc tggaagagga aggcaaggct gtcgccgaca gtatcaatgc
     1921 cgccggcggc aaggcggtgt tcatggcgct gaacgtgacc gaggaggccg gttggaagaa
     1981 ggtcgtggat cagacagtcg ccgcgttcgg ccagctggac atcctggtca acaatgccgg
     2041 catcagcggc agcgccacca acgatctgct cgattccgcc ctgtgggagc gggtgatggc
     2101 ggtgaacagc accggcgtct tcctcggcac cgcagccgcc gtgcggcaga tgcagcgcaa
     2161 cggcggcggg tcgatcgtca acctgtcctc gatctccggc gtggtcggcc agacgatggt
     2221 gcacatgggc tacaatgctg cgaaaggtgc cgtgcggatc atgaccaaat cgacggctgt
     2281 gcagttcggc aaggaccgca tccgctgcaa ttcggtgcat cccggactga tgccgccgat
     2341 gcgcacctcc ggcgccaccg ccgatccggc tgtccgtgcc agaatgctga aagcggtgcc
     2401 gctgggccgc accggcgagg tggacgaagt cgccaacgcc atcctgttcc tggcctcgga
     2461 cgaggcgtcc tatatcaccg gtgccgaact ctacgtcgac ggcggctatc tggcgtcgta
     2521 gccggccgcg gcaacatctt gaagcggttt ctgcccacct tggttcgcag acatcgcctt
     2581 aagtccattg tttgagggcg tgattcacct ttccggtgat cccacctgca aggattgcgc
     2641 cctaaaggga gaccgtcatg aaaatccttc gttcgtcagc ccggaagaca aaccgcgccc
     2701 ccgcatccaa cttcaccgga acggtatttt ccgatgaggt ggtggtcggc accgcgccct
     2761 cacggatgcg cggctccgtc gtctccttca cccccggcgc tcggacggca tggcacagcc
     2821 atccggtcgg ccagacgctg ttctgcctgt ccggtgccgg acgcgtgcag cgagaaggcg
     2881 aacaggtgca ggaaattcgc cccggcgaca ccgtggtcat accgcccgat gtaaggcatt
     2941 ggcacgggtc cgcccccgac aagctgtttt cccacctcgc gatgtcggaa ctgaacgaca
     3001 agggagaagg cactgcctgg ttcgagcatg tgtcggacga ggattacaac gcgccacccg
     3061 caccgatgac ctgacccaac gacctcggcg cccgtcatgg caccggctat gcggccgccc
     3121 cacatccctc tggcttcagc acccgtttcg tcgaagccgg gtgcttggcc agcatgtggg
     3181 ctgggcgaac cgggcgtcgg acgaactcgc cgccacagtt cggacaaatg ccgtgcaacc
     3241 gatgctctac gcaatcggcg cagaacgtgc actcataggt gcagatccga gcctcctgac
     3301 tgtcgggcgg aagatcgcgg tcgcagcatt cgcagttggg gcgcaattcc agcatt
//
LOCUS       DASWQN010000310         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_822878, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000310 DASWQN010000000
VERSION     DASWQN010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822878"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VGC82_05135"
     CDS             complement(<1..1025)
                     /locus_tag="VGC82_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0422689.1"
                     /translation="MSAELPQSGAEILLRALKDQGVDVIFGYPGGAVLPIYDALFNQN
                     AIRHVLVRQEGGAVHAAEGYARSTGKVGVVLVTSGPGATNAVTGLVDALMDSIPVVCL
                     TGQVPTHLIGNDAFQEADTTGITRPATKHNYLVKRPEDLARVVHEAFYVARSGRPGPV
                     VIDLPKDIVINPAPYAEPTFNHPSYRPRTDPDPGQIAKAVSLMKQAKRPMIYAGGGVI
                     NSGPEASEALTKLTRLTGFPITNTLMGLGCYPASDPQFLGMLGMHGTYEANLAMHGCD
                     MLLAVGARFDDRVTGRLNAFSQDSRKIHIDIDGSSINKNVPVEVPIMADAGKALNALL
                     AAWEASD"
     gene            complement(1071..2072)
                     /gene="miaA"
                     /locus_tag="VGC82_05140"
     CDS             complement(1071..2072)
                     /gene="miaA"
                     /locus_tag="VGC82_05140"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018979911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY0422690.1"
                     /translation="MTCQPVSPVVTDSRPHALIVAGPTASGKSALAIAIAERLGGTVI
                     NTDSMQVYQELRILTARPTRDDEVRVPHRLYGVRPASDAGSVAWWRGQALLAMEEARI
                     TNRLPILTGGTGMYFAALTSGLSEIPDPGADARTEARRLLAEHGPAALHASLSHVDPA
                     TAARLKPEDSQRIARAWEVWRGTGVGLAAWQSRRSAPAPWRFTAIRLDPPREALRAAI
                     ATRFTAMLRDGALQEVRDLMALDLEPSLPAMRAHGVPELSAHLRGLMSLEEARRRTEL
                     ATSQYTKRQATWFRHHSPASQTRTFLLNARIACTAQFSKREWDDLVMFIQNGVDQQR"
BASE COUNT          377 a          661 c          668 g          391 t
ORIGIN      
        1 tcatcgctcg cttcccacgc cgccagcagc gcattcagcg ccttgccggc atcggccatg
       61 atcggtactt cgaccggcac gttcttgttg atcgaggatc cgtcgatatc gatgtggatt
      121 ttccgcgaat cctggctgaa ggcgttcagc cgcccagtca cccgatcatc gaacctggcg
      181 ccaacggcca acagcatgtc gcagccatgc attgccagat tggcttcata ggtcccgtgc
      241 atccccagca tcccaaggaa ctgcgggtcg ctcgccggat agcagcctag gcccatcagc
      301 gtattggtga tcggaaagcc ggtgagacgc gtcagcttcg tcaaagcctc cgacgcttcc
      361 gggccggagt tgatgacgcc gccgcccgca tagatcatcg gccgcttggc ctgcttcatt
      421 aacgaaaccg ccttggcgat ctgtccggga tcgggatcgg tgcggggccg gtagctagga
      481 tggttgaatg tcggctccgc atacggagcg ggattgatca cgatatcctt gggcagatcg
      541 atcaccaccg ggccgggacg gccgctgcga gcaacgtaga acgcctcatg caccacacgc
      601 gccaggtctt cgggccgctt gaccagataa ttgtgtttcg ttgcgggccg cgtgatgccg
      661 gtggtatccg cttcctggaa cgcgtcgttg ccgatcaggt gcgtcggcac ctggccggtc
      721 aggcaaacca cggggatgct gtccatcagc gcatcgacca ggccggttac cgcattcgta
      781 gcgcccgggc ctgatgtaac cagcaccacg ccgaccttgc cggtggagcg ggcgtaaccc
      841 tcggcggcat gtacggcgcc gccttcctgg cggaccagga cgtggcgaat ggcgttctgg
      901 ttgaacaacg cgtcgtagat cggcaagacg gcgccgccgg ggtagccgaa aataacgtcc
      961 acgccctggt ccttgagagc acgtaaaagg atctctgctc ccgactgggg caattccgcc
     1021 gacatcggtt cggctcctgt gtttgaatga agagcggacg gataagcctc ttaacgctgt
     1081 tggtcaaccc cgttttgaat aaacatgacc aaatcgtccc attctctttt cgaaaattgc
     1141 gctgtgcagg cgatacgcgc atttaataga aaagtgcggg tctgggatgc gggagagtgg
     1201 tggcggaacc atgtcgcctg ccgcttggtg tactggctcg tagcaagttc cgtccggcga
     1261 cgggcttcct ccaacgacat caagccacgc aagtgggccg acagttccgg aaccccatgc
     1321 gcacgcatcg ccggcaggga aggttccaga tcgagggcca tcaggtcgcg cacctcctgc
     1381 aatgcgccgt cgcgcaacat cgccgtgaag cgcgtggcga tggccgcccg cagcgcctcg
     1441 cgcggcgggt ccagccggat cgcggtgaaa cgccatggcg ccggggcgct ccggcggctc
     1501 tgccaggcgg cgaggccaac gccagtgccg cgccaaacct cccaggcgcg ggcgatacgc
     1561 tgactgtctt ccggcttcag gcgggcggcg gttgccggat cgacatggct caggctggca
     1621 tgcagggcgg cgggtccatg ttcggctaac aatctgcgcg cctcggttct tgcatcggct
     1681 ccgggatcgg gtatctcgct cagcccgctg gtcagggccg cgaaatacat ccctgtcccg
     1741 cccgtcagga tcggcaaccg gttcgttata cgggcttcct ccattgccag cagcgcttgt
     1801 cctcgccacc acgcgacgct gccggcgtcg gatgcagggc ggacgccata gagccgatgc
     1861 ggaacccgaa cctcgtcgtc ccgggtcgga cgagcggtga gaatccgtag ctcctgatac
     1921 acctgcatcg aatccgtatt gatcacggtg ccacccaggc gctcagcgat ggcaatcgcg
     1981 agagcggatt taccactggc ggtgggcccg gcgacgataa gggcatgcgg ccgggagtcg
     2041 gtcaccactg gactaacggg ttgacaggtc atggcgctgc aaattgtgtg tcggggc
//
LOCUS       DASWQN010000311         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1482075, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000311 DASWQN010000000
VERSION     DASWQN010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482075"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VGC82_05145"
     CDS             complement(<1..314)
                     /locus_tag="VGC82_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY0422691.1"
                     /translation="MSRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATAFNAKKHQVIE
                     GKGVLNNRISEYLFQHLNDIGVPTHFIRRINMREQLIREVEIVPLEVVVRNVAAGSLS
                     QRL"
     gene            618..935
                     /locus_tag="VGC82_05150"
     CDS             618..935
                     /locus_tag="VGC82_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HEY0422692.1"
                     /translation="MTTFDKREEGYENKFAHDEELRFRAQARRNKLLGLWVAEQLGFS
                     ADMAADYARTVVAAGTAGDLDVVRKVMSDFDVKGVASTEPQIRARMDELMAQAVAQVQ
                     AGV"
     gene            complement(1045..1533)
                     /gene="cynS"
                     /locus_tag="VGC82_05155"
     CDS             complement(1045..1533)
                     /gene="cynS"
                     /locus_tag="VGC82_05155"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEY0422693.1"
                     /translation="MKRCDLTEKLLDIKRDNGWSWKYICEKIGGYSEVLIVGAILGQM
                     KLTKPQAANAGELFALTKSEIAMLNETPMRGEGTPMPPTDPLIYRFYELVMVNGPAWK
                     ALIEEEYGDGIMSAIDFDMVLERLPNPKGDRVKITMSGKFLPYKYYGASGNVPEYGFK
                     EG"
     gene            complement(1806..>2249)
                     /locus_tag="VGC82_05160"
     CDS             complement(1806..>2249)
                     /locus_tag="VGC82_05160"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEY0422694.1"
                     /translation="RKGPAKGGVLDGGQATRLRALLAEHGQGALPSESGTVRGLARLL
                     RRNPTDAERILWQALTSDGRFAGQFKRQTPVGRHIPDFVSFVHRVAIELINPDESEVI
                     AKDRAARQAWLEARDYRVVHVQVAAVERDLASELDRLESSVLERR"
BASE COUNT          406 a          714 c          685 g          444 t
ORIGIN      
        1 aggcgctgcg acagcgatcc cgccgccaca ttccgcacca cgacctcgag cggcacgatc
       61 tccacttcgc ggatcagctg ctcgcgcatg ttgatgcggc ggatgaaatg agtcggcacg
      121 ccgatgtcgt tgaggtgctg aaacaggtac tccgaaatcc ggttgttgag gacgcccttg
      181 ccctcgatca cctggtgttt cttggcattg aacgcggttg catcgtcctt gaagtgctga
      241 atcagggttc ccggttccgg tccttcgtac aggaccttgg ccttgccttc ataaatgcgg
      301 cgtcgacggc tcattgggat gtaccgtgtg ttgttgaaat ccatgaattt ggtgtgctcc
      361 ggatgacaag gatggagacc cacgacggag ctgccgtgaa ggccaggaag gggaaacaaa
      421 cgcgaaccgg gcccgattcc agcctatctg attggcccac ttagtacaat cgatcaggtc
      481 ccgggagcgc cagttttacc ctccgaacct gcgaccttca ggctgttgtt ttaaccagcc
      541 actcccgtaa tctaagcacg gcaaaaggct ggcgcaacgc gtgccgccat ttcagcccag
      601 ggatcggaac cctcgaaatg accacattcg acaagcgcga ggaaggctac gagaacaagt
      661 tcgctcacga cgaggagctg aggttcaggg cccaggcacg ccgcaacaag ctgcttggcc
      721 tttgggtggc ggaacagctc ggcttttccg cagacatggc cgccgattat gccagaaccg
      781 tggtcgcagc cgggacagcg ggcgacctcg atgtggtccg caaggtgatg agcgatttcg
      841 acgtcaaggg cgtcgccagc accgaaccgc agattcgggc cagaatggac gaactgatgg
      901 cgcaggccgt cgcccaggtg caggcgggag tgtaaggccc cgcctcatcc ggggcgtgca
      961 aagcacgaac gaggaatctc gagcttccgg atcgtcgctt cgcaccatcc ggaatgacgt
     1021 ttccgtcggg actcgacggc aacgtcagcc ctccttgaac ccatattccg gcacgttgcc
     1081 gctggcgccg taatatttgt acggcaggaa cttgcccgac atcgtgatct tgacccggtc
     1141 ccccttgggg ttgggcagcc gctccaacac catgtcgaag tcgatcgccg acatgatgcc
     1201 gtcgccgtat tcctcctcga tcagcgcctt ccaggccggg ccgttaacca tcaccagttc
     1261 gtagaagcga tagatcaggg gatcggtcgg cggcatcggg gtgccctcgc cgcgcatcgg
     1321 cgtctcgttc agcatcgcga tctcggattt ggtcagcgcg aacagctcgc cggcgttcgc
     1381 cgcctgcggt ttggtcagct tcatctggcc cagaatggcg ccgacgatca ggacttcgga
     1441 atagccgccg atcttctcgc agatgtattt ccagctccag ccattgtcgc gcttgatgtc
     1501 gagcaacttt tcggtcaggt cgcatcgttt catggatcgt ctcccttcca ctccgccatg
     1561 cccgggctgg tcccgggcat ccacatttcg cttgagattt cacgaggaca tcgacggtgc
     1621 cgcgacgggc ccgagcgtga cgcctgcttt gatgcttatt ctctttcaat gcctacaata
     1681 ctgagcgcct gtttcctgca cgaagcatgc cagataaaac cgccgccggc gactgcgcga
     1741 atggttgcgc gctgttgtcg ggcgcggtgg tgcccggaca gaacccgcgc ggatctttcg
     1801 gtcgcttagc ggcgctccag cacgcttgat tccaggcggt caagttcgct ggccagatcg
     1861 cgctcgacag cggcgacctg cacatggacg acgcgataat cgcgcgcctc cagccaggcc
     1921 tgccgcgccg cacggtcttt ggcgatgacc tcgctctcgt ccggattgat cagttcgatc
     1981 gccacgcggt gcacgaaaga gacgaagtcc ggaatgtggc ggccgaccgg cgtctggcgc
     2041 ttgaactggc cggcgaagcg gccatcggag gtcagcgctt gccacaggat gcgttcggcg
     2101 tcggtcggat ttcgccgcag cagccgcgcc agcccgcgca cggtgccgct ttccgacggc
     2161 agcgcaccct gcccgtgctc ggcgagcagc gcgcgcagcc gtgtcgcctg tccgccgtcg
     2221 agcacgccgc ccttggccgg gcccttgcg
//
LOCUS       DASWQN010000312         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_162940, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000312 DASWQN010000000
VERSION     DASWQN010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162940"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..365)
                     /locus_tag="VGC82_05165"
     CDS             complement(15..365)
                     /locus_tag="VGC82_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422695.1"
                     /translation="MDEIVTPHSIARALHDLQALRKAIPPENPELADLANRAQRAVLE
                     LTLRGIDDANRRAMMLASSGVLEHEDGSMTVEEIFQYDKPHLIAAEAVANAIGWLETS
                     GHVIIKDPRLGDGN"
     gene            complement(628..1578)
                     /locus_tag="VGC82_05170"
     CDS             complement(628..1578)
                     /locus_tag="VGC82_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0422696.1"
                     /translation="MTEVKAVVVDHSKPDRLAIRSMPLMPAYRDEVTVRVRAISLNRG
                     EVSRAVRSAEPGWRPGWDFAGTIDATAPDGSGPVKGTRVVGILPSGAWAEVIRVPKDS
                     VAPLPDQVSDAQAAALPVAGLTALHALRQGGLLLGRKVLIDGASGGVGHLACQLAAAS
                     GAIVHGHVRNTQYKSMVEEWCGERTIVGPHLSAAAAFGPYHLILDSVGGSALGAALGM
                     LRPDGTCVTFGVSEAPTANFDSGAFFRSGGSRLYGLVLFPELRKVEPASEGLAILAAM
                     IARGALKPRVEIEANWSEIGSVARQLLDRSFMGKAVLHLN"
     gene            2014..>2901
                     /locus_tag="VGC82_05175"
     CDS             2014..>2901
                     /locus_tag="VGC82_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0422697.1"
                     /translation="MEQTISRRAALSGALAAGTSLALTRRAKAADQTVRIGVLTDMTG
                     TYAAITGKGCVLGAQLAIEDFARVHPEIKVELVSADMLLKPDVALSIAGDWFDNKGVD
                     LLTDLPLSSAAIAVGEMAKAKDKLAIYSGAASSILTGERCGPNHLHWSYDTWGIPHAI
                     VDSAVKEGADSWFFITADYAYGQQLERDSASFVTTAGGKVVGSVRYPFPGTMDFSSFL
                     LSAKTSGAKVIGLANGGVDAVNCVKQAAEFGIMDGGQRVVGVGLLLNDILSIGLKAPQ
                     GVSLAEPYYWDLNDGTRAFG"
BASE COUNT          549 a          922 c          888 g          542 t
ORIGIN      
        1 ggcccgagcg tggatcagtt accgtcgccc agccgagggt ctttgattat cacgtggcca
       61 gaggtttcca gccatccaat ggcgttggcg acggcttccg cagcaatgag atgcggttta
      121 tcgtactgaa atatctcctc gaccgtcatt gaaccatctt cgtgttccag gacaccggag
      181 gacgcaagca tcatggcccg gcggttggca tcatcgatcc ctcgcaaggt gagttcgaga
      241 accgcgcgct gcgctctgtt tgcaaggtca gcgagttccg ggttctccgg cggaattgcc
      301 tttctcagcg cctgaaggtc gtgaagagcg cgtgcaatcg agtgcggcgt gacaatctcg
      361 tccatacatg gctccgggca gattgggcag cataatccgg ggcccgccaa gcgagcaacg
      421 gccggcgtga ccagacgcca gcctgcatac cggtcggcga agcgaagcag ccggtcgggc
      481 gcaggcgtcg gacataagtg ccgtggacac ggcacgcgtc agaagcctcg ccatccatct
      541 gctgtccaaa tacgggttca cttccgatgc ggtagaagcc ttccgttgca gcgcgtcgat
      601 gtccggcaga agccccagaa gcggagccta attcaggtgc agcacagcct tgcccatgaa
      661 gctacggtcc agaagctgcc gcgcgacgct gccgatctcg ctccagttgg cttcgatctc
      721 gaccctgggt ttcagtgctc cgcgcgcgat catcgcagcc aagatcgcca gcccctccga
      781 tgccggctcg accttgcgca gttcgggaaa cagcaccagc ccataaagcc gtgacccgcc
      841 cgaccggaag aacgccccgc tatcgaagtt cgccgtcggc gcctccgata caccgaacgt
      901 gacgcaggtt ccgtctgggc gcagcatccc caatgccgcg ccaagcgcag agcctcccac
      961 ggagtccagg atcaggtgat atggcccgaa agcagccgcc gcgctcaggt gcggtccgac
     1021 gatggtgcgt tcgccgcacc attcctccac catcgacttg tattgcgtat tgcgaacgtg
     1081 gccatggacg attgcccctg aggcggcggc aagctggcat gccaggtgcc caacgccgcc
     1141 agaagcgccg tcgataagca ccttccgtcc cagcagcagg ccgccctgcc gaagcgcatg
     1201 gagcgccgtt agcccagcca ccggcaaggc ggcagcctga gcgtcgctca cctggtccgg
     1261 cagcggcgcg accgagtcct tcggcacgcg gatcacctct gcccaggcac ccgatggcag
     1321 aatccccacc actcgcgtcc ccttcacggg accgctgcca tccggcgccg tcgcatcgat
     1381 cgtgcccgca aagtcccagc cgggtcgcca gccaggttcg gccgaccgta ccgctcgact
     1441 gacttctccc cggttcaggg aaattgcccg cacgcgcacc gtgacctcat cgcgatatgc
     1501 cggcatgagc ggcattgatc ggatcgccag tcggtcgggc tttgaatggt caacgacaac
     1561 agctttcact tccgtcatgg gcgacttcct caatccttgg ctttattccg atgaccgccg
     1621 gatccttgcc ggtgcgactg ccgcggcgca agagcccaat gaagcagact cagtgttcct
     1681 aggtaatcac agggtggctc gtgattctat gggcaatctt gccgggcggg ttacgcggca
     1741 agctggccga gattggcgca ttctcgcggt gcgccgacat tcgcgccacc gcatgctgcc
     1801 agatccggct gccccgcggg gttgcaacgc cgcgttccgt caaggtccgc gcgcgtggtg
     1861 gtgacaagat gttcacctgc tgcggccatt cggggcgggt gccaaaaggc cgcgctcgtt
     1921 caaggtcaat gtccccagca gagttagcac tggacagatt tatgccaaag cggcaaatcc
     1981 ctggatcaaa tggatgcgcc gccgggagga aatatggagc agaccatcag ccgccgggca
     2041 gcgctcagtg gagcccttgc cgccggcacc agtcttgcgt tgacgcgccg cgccaaagcg
     2101 gcagatcaga ccgtccgcat cggcgtgctc accgacatga ccggcactta tgcggcgatc
     2161 accggaaaag gttgcgtact tggggcgcag cttgccattg aggatttcgc cagggtccat
     2221 ccggagatta aggtagagtt ggtgtcggct gatatgctgc tgaagccaga cgtcgcgcta
     2281 tcgattgccg gcgattggtt cgacaataag ggcgtcgacc tgctgaccga cctcccgctg
     2341 tcgtccgccg caattgccgt cggcgaaatg gcgaaggcca aggacaagct ggccatctac
     2401 agcggtgccg cctcgtccat cctgaccggc gaacgctgtg gtccgaacca cctgcattgg
     2461 tcgtacgaca cttggggcat cccgcatgcc atcgtggaca gtgccgtaaa ggagggtgcc
     2521 gattcctggt tttttatcac cgccgattat gcctacggcc aacagttgga gcgtgactcg
     2581 gcttcattcg tcaccaccgc cggcggcaag gtggtcggtt cggtgcgcta tccgtttccc
     2641 ggaacgatgg atttctcgtc cttcctgctg tcggcgaaaa ccagtggggc aaaagttatc
     2701 gggctcgcca acggcggcgt cgatgcggtg aattgcgtaa agcaggcagc cgaattcggc
     2761 attatggatg gcggtcaaag ggttgttggc gtgggactgc tgctgaacga tatcttaagc
     2821 ataggcctca aggcgccaca gggcgtcagt ctagccgaac cctactattg ggatctgaac
     2881 gacgggacgc gagcgtttgg t
//
LOCUS       DASWQN010000313         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2405694, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000313 DASWQN010000000
VERSION     DASWQN010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405694"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VGC82_05180"
     CDS             complement(<1..223)
                     /locus_tag="VGC82_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin deacetylase"
                     /protein_id="HEY0422698.1"
                     /translation="MQRMDRDLIGYGANPPDPKWPNRSRVAVNFVLNFEEGSEPSVPD
                     GDPVSEWGLTEYGGASPGVVGRDLAAEGMF"
     gene            341..1279
                     /locus_tag="VGC82_05185"
     CDS             341..1279
                     /locus_tag="VGC82_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HEY0422699.1"
                     /translation="MSDIAAPWIISEGLAGLRSQALGLAEAAGLAPEIRELKPGVPWK
                     WIAAKFWPNPLGAVADAVRAPLPSLAIGCGGMAGAVLAALRRKSMRVVQVQNPRMDIG
                     RFDLIIANRHDDLTGPNVFVTRTALHRVTASRLAAEAEAWRDRFSLYRRPLVAVLLGG
                     NNGRYRLDLASGARLAGDLARMAERDKVGVVVTPSRRTDPAVTDLMRTALEPHGGWVW
                     DSRGDNPYFGMLALADLIIVTQDSMSMISEAAATATPVMFASLPGFWRRQGLFLQPLL
                     DEDRIRPFEGRFSTWPVSPLNDTPLAAAEMCRRLGL"
     gene            1288..>2391
                     /locus_tag="VGC82_05190"
     CDS             1288..>2391
                     /locus_tag="VGC82_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422700.1"
                     /translation="MLRIQTFDARAGGNVIYKALAHPLAAEAIGRLYARFEGPVALYD
                     PDGIADALLAMYPAAFDSLFVHDVTAIGQSRGGLDARALTEIGVSGARTVLIAAFDAS
                     RVVARIAHMLPAGANVLTLDDARLPDALLTNRSRYLDKLNFATNFAFFRDADGLHTRL
                     ASANYWASYGAANVRLWLRLFDTDGTILATWEQALSSGPGGFSIDSGQVRERFGLAPF
                     TGQLFIHAVGVTGHDVVKYALDTYASDNGASLSCTHDANAWPSDRFAGLPAPRPDEKV
                     VLWLQNSHAVPIPPGTIALDRMGAESPAPLTQEVGPFATVAMDVADFLPGLHWPAQIE
                     IRTGRHVVRPRYEVTRNDRVRIAHVNVERDNLAV"
BASE COUNT          391 a          768 c          789 g          443 t
ORIGIN      
        1 cgaacatgcc ttccgcggct aggtcgcgcc cgacgacacc agggctggcg ccgccatact
       61 cggtcagtcc ccattccgaa acaggatctc cgtcgggaac agagggctcc gagccttcct
      121 cgaagttcag cacgaaattc actgcgacgc gggagcggtt cggccatttc gggtcaggag
      181 ggttggcgcc gtagccgatc aggtcgcgat ccatccgctg tatcctgttc catgaagccg
      241 gcaggataca ggcgaaagcc acacggtgcg aaggccggat ctttgtttgg atgtggctga
      301 cacgttgacg tttccggatg tggcgccgaa aagcccgctg atgtctgaca tcgccgctcc
      361 ctggattatc agcgagggtc tggccgggtt gcgctcgcaa gccttgggcc tggccgaggc
      421 tgccggactg gcgcccgaaa tacgcgagtt gaagcccggc gtcccatgga aatggatcgc
      481 tgcaaaattc tggcccaatc cattgggtgc cgtcgccgac gccgtgcggg cgccgttgcc
      541 atccctggcg atcggctgtg gcggcatggc cggagccgtg ctggcggcgc tgcgacggaa
      601 gtcgatgcgg gtggtgcagg tgcagaaccc gcgcatggac atcggtcgct tcgacctgat
      661 catcgccaac cgtcacgacg acctgaccgg acccaacgtc ttcgtcaccc gcaccgcctt
      721 gcaccgggtc acagcatcgc gtctggcggc ggaggcggaa gcctggcgag accgcttcag
      781 cctttaccgg cggcctctgg tcgcggtgct actgggtggc aacaacggcc gctaccggtt
      841 ggacctggct tccggcgcac gactggccgg tgatctggcc agaatggccg agcgggacaa
      901 ggttggcgtg gtggtgacgc cgtcacggcg aaccgatccg gcggtgaccg acctgatgcg
      961 tacggccttg gagccgcacg gcggctgggt ctgggattcc cggggagata atccctattt
     1021 cgggatgttg gcgttggccg acttgatcat cgtgacacag gacagcatgt cgatgatctc
     1081 cgaggccgcg gcgaccgcga ccccggtcat gttcgcgtcg ttgcccggct tttggcggcg
     1141 tcagggcctg tttctgcaac ctcttttgga cgaagatcgt attcgccctt tcgagggccg
     1201 cttctcgacg tggccggtat cgccactaaa cgatactcca ctcgccgccg ccgaaatgtg
     1261 ccggcggctc ggactctaag gacagacatg ctgcgaattc agactttcga cgcgcgcgcc
     1321 gggggtaacg tcatctacaa ggctttggcc catccgctgg cggcagaagc gatcggccgg
     1381 ctttatgcgc gttttgaggg cccggtcgcc ctgtacgacc ccgatgggat cgccgacgca
     1441 ttgttggcga tgtacccggc ggcattcgac agcctgttcg tccacgacgt cacggcgatc
     1501 gggcaaagcc gcggcggact cgatgcccgc gcgttgaccg agatcggcgt gagcggcgcc
     1561 cgcacggtgc taatcgcggc tttcgacgcc tcccgtgtcg tcgcccgcat cgcccatatg
     1621 ctgccggccg gagccaatgt gctgacgctg gacgacgcgc ggttgccgga cgcgttgttg
     1681 accaaccggt cccgttatct ggacaagctg aatttcgcga cgaacttcgc attcttccgc
     1741 gatgcggatg ggctgcacac gcggctcgcc tcggccaact actgggcaag ctacggcgcg
     1801 gcgaacgtgc ggctgtggct gcggctgttc gacaccgatg gcaccatcct ggcgacctgg
     1861 gagcaagcgc tgtcatccgg ccccggcgga ttctccattg acagcggcca ggttcgggaa
     1921 cggtttgggc tggcaccctt taccggccag ctcttcattc acgccgtcgg ggtcaccggc
     1981 cacgacgtgg tgaaatacgc cctggatacc tatgcatccg acaacggggc ctccctgtcc
     2041 tgcacccatg atgcgaatgc ctggccgtcg gatcgtttcg ccggcctgcc cgcaccccga
     2101 ccggacgaga aggtggtgct gtggctgcag aacagccacg cggtgccgat cccgcctggc
     2161 acgatcgcgc tcgaccgaat gggggcagag agcccggcgc cactgacgca agaagtcggg
     2221 ccattcgcga ccgttgcgat ggatgtcgcg gatttccttc ccggcctgca ctggccggcg
     2281 cagatcgaga tccgcaccgg tcggcatgtc gtgcggccga gatatgaggt gacccggaac
     2341 gaccgcgtcc gcatcgctca tgtcaacgtg gagcgggaca atctggccgt t
//
LOCUS       DASWQN010000314         3109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_294937, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000314 DASWQN010000000
VERSION     DASWQN010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294937"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VGC82_05195"
     CDS             complement(<1..290)
                     /locus_tag="VGC82_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422701.1"
                     /translation="MPTSRRDSAALGQWSANARRTRMPFLDAKGDLQQTQPDDGEFPA
                     DLRGEPDAIKECWVPTDCHESAGWVFETRVRRSPTPRTGGKGPATTTWGNLAP"
     gene            573..1319
                     /locus_tag="VGC82_05200"
     CDS             573..1319
                     /locus_tag="VGC82_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0422702.1"
                     /translation="MRGWLANSAGRPATEDAVAAMNVQDVQVRLGAHLALDGVTGRFD
                     PGSLTAVIGPNGAGKSTLLNVLSGLLRPTSGRVTCSALLRNRVAYLPQQTELDRDFPM
                     TVAELVALGLWHTIGAYRGLSRSASGRVADAIEAVGLQEARHRRIGDLSVGQIRRSFF
                     ARLLLQDAEVILLDEPFAAVDARTVETLLALIARWHADGRTVVAVVHDMRQVRAHFPS
                     ALLLSRRPVAWGSTGAVLTDDNLAWAAAGA"
     gene            1298..2182
                     /locus_tag="VGC82_05205"
     CDS             1298..2182
                     /locus_tag="VGC82_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY0422703.1"
                     /translation="MGRCWRMTLSLYDLLITPFEGFSFMRVALAACLALALSGGPLGV
                     LLLLRRMALDGNVLSHAVMPGAALGFVYAGHSPAALSLGGFASGVMVAGLTGLLGRKR
                     ATRQDAGLVVFYLVALALGVTLVAFFGSNVDTVRVLFGTVLAINPGALMQIASVSTMT
                     MLALAVFYRSIMVDAFDPVFLRLAGVQGMPALFMLLVLLNLVVSCQTFGTLLAIGPIL
                     LPAAAARCWTNRPGLMMALAVLIGMLSSYAGLLLSYHRNLPSGPAIVMVAGALYGASV
                     LVSSGVAVVRTRLKGNGR"
     gene            2179..3102
                     /locus_tag="VGC82_05210"
     CDS             2179..3102
                     /locus_tag="VGC82_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEY0422704.1"
                     /translation="MKRRALLSSLAVVALPLAPSVALAADKMKVVATFSVLGDMVGRI
                     GGDQIDLVTIVGPASDTEEYEPTAADIPRIANARILFMNGLNDEFEPWLDNLMKQARF
                     TGIKAIATRGAKAISAEDEHPIGGRPKASALDQHAWMDPRNGVVYVKNIADTLIRADA
                     ANADLYRNRSASYIKDLRDLHSWALAEIAAVPVSKRRVLASHDSLQYLALAYGITMIS
                     INGWTNKAEPSAADLARLADQITRDHVKALFLDSITDPRAMERIGKETGAVIGGTLYG
                     DALSKPGGEAPTYIDMIRHDVSTLKAGMRLN"
BASE COUNT          490 a          980 c         1029 g          610 t
ORIGIN      
        1 ggcgccaggt tgccccatgt cgtcgtcgca ggtcctttgc cgcctgtacg gggcgtcggg
       61 gagcgcctga cccgggtttc gaagacccaa ccggccgact cgtggcaatc ggtcggcacc
      121 cagcattcct tgatcgcgtc gggttcgcct cgcaggtccg cgggaaattc tccatcatcc
      181 ggctgcgttt gttgaaggtc gcctttcgca tcaagaaatg gcatccgggt ccggcgggcg
      241 ttcgctgacc attgccccaa cgccgcactg tcccggcggc tggtcggcac gttgatcccg
      301 cgtccggctc gagcgacggg gcgggtcagg cctttcagtc gaatctgaaa cgcggtccaa
      361 ccagcagaga cgtaggcacg accatgttgt gcttgagtag tcacacgagt tctacgcggc
      421 accgtgacta agatgacgtg aataagatga gctgacccgc aatgcatggt gggcacaagt
      481 cccgtggcac ggtgatgttg gtgcgcaacg gtctgctcga cgcgtccgcc gtgcgatgtc
      541 attgttccga atccaatggg tggatttcgg caatgcgagg atggctcgcc aacagcgctg
      601 gccgaccggc caccgaggac gcggtcgccg ccatgaacgt gcaggacgtg caggtccgcc
      661 tgggcgcgca cctggcgctg gacggcgtca ccgggcgttt cgacccgggc agtctgaccg
      721 ccgtcatcgg gccgaatggc gccggcaaga gcacgttgct gaacgtcctg tccggcctgc
      781 tgcgcccgac aagcggccgc gtgacctgtt cggctctgct gcgcaaccgc gtcgcctatc
      841 tgccgcagca gacagagctg gaccgggatt ttcccatgac agtggcggag cttgtggccc
      901 tgggcttgtg gcatacgatc ggcgcttacc gcggcctttc gcggtccgcg agcggtcggg
      961 tcgccgatgc gatcgaggca gtcggcctgc aggaagccag acaccgccgg atcggagacc
     1021 tctcggtcgg acagatacgc cgatcctttt tcgcgcggct gttgctgcag gatgccgagg
     1081 tgatcctgct tgacgaaccg tttgccgcgg tcgatgcgcg cacggtcgaa accctgctgg
     1141 cgctgatcgc acgctggcac gccgacggcc gcaccgtcgt tgccgtggtg cacgacatgc
     1201 ggcaggtgcg ggcgcatttc cccagcgcgc tgctgttgtc gcggcgtccc gtcgcctggg
     1261 gcagcaccgg cgccgtcctg accgacgata acctggcgtg ggccgctgct ggcgcatgac
     1321 gttgtcgctg tatgacctgc tgatcacgcc cttcgagggc ttcagcttca tgcgcgtcgc
     1381 cctcgccgcc tgtcttgcgc tggccctgtc cggcggcccg ctgggtgtgc tgttgctgct
     1441 gcggcgaatg gcactggacg gtaacgttct gtctcacgcc gttatgcctg gcgcggctct
     1501 gggcttcgtc tatgccggcc attcgccggc ggcgcttagt ctgggtggct tcgccagtgg
     1561 cgtaatggtc gccggcctga ccggactgct gggccggaaa cgtgcgacgc gccaggacgc
     1621 tggccttgtc gtcttctacc tcgtcgccct cgcgctcggt gtcactctgg tggcgttctt
     1681 cggcagcaat gtcgacacgg tgcgcgtgct gtttggcacg gtcctggcga tcaatccagg
     1741 cgccctgatg cagattgcct ccgtcagcac catgacgatg ctggcgctgg cggtgttcta
     1801 caggtccatc atggtggatg cgttcgatcc ggtgttcctt cggttggctg gcgttcaggg
     1861 gatgccggcg ctgttcatgc tgctggtgct gctgaacctc gtcgtcagct gccaaacgtt
     1921 tggcacgttg ctggcgattg gcccgatcct tctacccgcc gccgcggccc gctgctggac
     1981 caaccgccct ggcctcatga tggcgttggc ggtgctgatc gggatgctgt cctcttacgc
     2041 tggcctcttg ctgtcgtatc atcgcaacct gccgtccggg ccggcgatcg tcatggtcgc
     2101 aggcgcattg tacggcgcgt cggttctggt gtcgtccggc gtggcggtgg tgagaacacg
     2161 actgaagggg aacggtcgat gaaacgacgc gctttgctga gcagccttgc ggtcgtcgcc
     2221 ttgccgctgg cgccgtcggt ggcgctggca gccgataaga tgaaggtcgt tgccacattc
     2281 agcgtcctgg gcgacatggt cggccgcatc ggcggcgacc agatcgatct ggtgacgatc
     2341 gtcgggcccg ctagcgacac cgaggaatat gaaccaactg ccgccgatat cccgcgcatc
     2401 gcgaacgcgc gcattctatt catgaacggc ttgaacgacg agttcgagcc gtggctggac
     2461 aatctgatga agcaggcgag gttcaccggc atcaaagcga tcgccacccg cggcgctaag
     2521 gcgatcagcg ccgaggatga gcatcctatc ggaggacgcc cgaaagccag cgcgctggac
     2581 cagcacgcct ggatggatcc acggaacggt gtcgtctatg tcaagaacat cgccgacacg
     2641 ctgattcgcg cggatgctgc caatgcggat ctgtaccgaa atcgttcggc gtcctacatc
     2701 aaggacctcc gggacctgca cagctgggca ctggcggaaa tcgcggctgt gccggtttcc
     2761 aaacggcgcg tgctggcctc tcatgattcg ctgcaatatc tagctctggc atatggaatc
     2821 accatgattt cgatcaatgg ctggaccaac aaggcggagc cctcggccgc tgacctcgcg
     2881 cgcctggccg accagatcac gcgggaccat gtgaaggcgc tattcctcga cagcatcacc
     2941 gatcccagag cgatggagcg gatcggcaag gaaaccggcg cggtgatcgg tggcacgctg
     3001 tacggcgatg cgttgtcgaa gcccggcggg gaggcgccca cctatatcga catgatccgc
     3061 cacgacgtct cgacactgaa ggccggcatg aggctgaact gacgccgaa
//
LOCUS       DASWQN010000315         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_690421, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000315 DASWQN010000000
VERSION     DASWQN010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690421"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..582)
                     /locus_tag="VGC82_05215"
     CDS             complement(283..582)
                     /locus_tag="VGC82_05215"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEY0422705.1"
                     /translation="MQMRIIWGKILPGQWDAFEAAFKKALEIRGEVKGLKGQWLLRDE
                     NDPDAGYSISQWESAEDMRAFWDSKKRSEAMAVLQPFYVNQYTITNCAVRIASSY"
     gene            complement(1051..1608)
                     /locus_tag="VGC82_05220"
     CDS             complement(1051..1608)
                     /locus_tag="VGC82_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY0422706.1"
                     /translation="MIIGYGRTSTFDQAAGLAAQERELKAVGGEKMFTEQVSSVAQRG
                     SLAECLAFLREGDVLMVTKPDRLARSTAELLAIEADLSKRKVGLVILSMGGEWLDTRN
                     PTSKLTLTILAGVATWEREIMLERQREGIAKAKSEGKYKGRPASIDAAEIKRLMATMG
                     PAAIAKHLGIARSTVYRLLEPAKAA"
BASE COUNT          391 a          609 c          564 g          435 t
ORIGIN      
        1 cgtgagatag acagtggaat tcccgcggcg ctgaagctgg ttccggtggg gtggaccacc
       61 tgccgcatcg ttggatcaga cgacgtcatc aacaactgcg ttatcgaccg catcgtcacc
      121 acgacaaagc caaagcggtg gcaatgattt cagcgggaaa cggtgctcag aacgttcgtt
      181 acggcgtaac gacgaaggtg gcattccact ctaggaatgc tggctcaaag ccaagaaacc
      241 cgcaaccggt aaccaagcgt tcccgatggt gtcagcgtcc ggtcaatatg aggatgcaat
      301 ccgaaccgca cagttggtga tcgtatattg gttcacgtag aacggctgaa gcacggccat
      361 ggcctcgctg cgtttcttgc tgtcccaaaa tgcccgcata tcctccgcgc tttcccactg
      421 cgagattgaa tacccggcat cgggatcatt ctcgtcccga aggagccatt gccctttcag
      481 ccccttcact tcgccgcgaa tttccagcgc cttcttgaag gcggcctcga aagcatccca
      541 ttgaccgggc aggattttgc cccagatgat ccgcatctgc atcgcgatac tccctccttt
      601 cggcgcccgg cgacaggtgt tgcaagtgac caccgggcat tgcccaaatg gcctctcaac
      661 ccgagtgcct gccgatagat tatcgtccgc ggcgtgcttt tacaaacgat cgatagaatt
      721 tatgaaaaga cgtgcgctag cagcaatgag taatagacac gagactccca gcacccagca
      781 agcaatgccc agagccacgc cttgacgggc tttgttgcag gcgaaatcaa taggatcgga
      841 ttgtccctgc cgagagcagc tttaggcgtt tcgcatggac gtcaattatg ggggaagcac
      901 atgataaagc cgcagcagcc gtggtggcga accaccaatc gcaccatcct tgtggtcact
      961 ctgctgctct gtgtggcggc cgcagtcggc ggcgcctact tattaggcct gctggaccaa
     1021 cttggcatgc tacattgacc tgaatggcac tcatgccgct ttggccggct ccagcagccg
     1081 atagacggtg ctccgcgcaa tgccgagatg cttggcgatg gcggctggac ccatcgtcgc
     1141 catcagtcgc ttaatctctg cggcgtcgat gctggccgga cggcccttgt atttgccctc
     1201 gctcttggcc ttggcgatgc cttcccgctg gcgctccagc atgatctcgc gctcccaggt
     1261 cgccacgccg gccaggatgg tcagcgtcag ctttgatgtc gggttgcggg tgtccagcca
     1321 ctcgccgccc atggacagga tcaccagacc gaccttccgc ttcgacaggt ccgcctcgat
     1381 cgccagcagc tccgccgtgc tgcgggccag gcgatcgggc ttggtgacca tcagcacgtc
     1441 accctcccgc aggaaggcca ggcactcggc cagactaccg cgttgggcaa cgctggaaac
     1501 ctgctcggta aacatctttt caccgcccac ggctttaagc tcgcgttcct gcgcggcgag
     1561 accggcggcc tgatcgaacg tgctggtgcg tccgtagccg atgatcattt tgcgccgcct
     1621 catgtccgat ttgcctctcg gtgttgtagg aggacatgta tgataagcca aatcatattc
     1681 aaatcggacg ctatttcatg tggtttatga tgtcccgtgt gggcaaggtc aaataggaca
     1741 gatcgcgttc ggctggcgcc atgttctggc cgccggccgc tgcgcgatct ccggctgcgt
     1801 tacgtcacgg atgggtttgg atggatcgaa tccacttgga tggcaaagcg acgttgtaac
     1861 agtcaggcac gcgttccgag ctgccactgc caccgcttct tccaactgta tagtccgttt
     1921 tcccgcgcgc ccagtgcctt gagcatgtcg tcaggtcgtt ctgcaccatc gttcagtcga
     1981 gtggcgccct ggaagggta
//
LOCUS       DASWQN010000316         6655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1350939, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000316 DASWQN010000000
VERSION     DASWQN010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350939"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..1177)
                     /locus_tag="VGC82_05225"
     CDS             complement(272..1177)
                     /locus_tag="VGC82_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0422707.1"
                     /translation="MVTWIPITIAAALFQCWRTALQQKLRHLLSVNGAGFVRFLYGAP
                     TALLLFLAGLWITAAPVPVPNLRFLLYCVAGGLLQILATNLLIMSFGYRNFAVGTAYG
                     KTEAAQSAVVALIILHEVLKPLSWIGIALGLSGVMTLSVAGRGIKPRELLAAIVQPAA
                     LCGLGAGFLFAFTTIFIKLANQSIPGPSLVAKALFAVLLTNMLQILMQGAWLLWREPV
                     ELRKAFTTWRTSMWAGTLSGCGSACWFTAFAMADVGLVRSVGQIEIVFTLLFSRFYLK
                     EQLKKGEVAGLVLVVGGVLLIVWGH"
     gene            1492..2142
                     /locus_tag="VGC82_05230"
     CDS             1492..2142
                     /locus_tag="VGC82_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein"
                     /protein_id="HEY0422708.1"
                     /translation="MTIEAIDQALPTGGIRLGALHEAASAGPDTEHAAAATLFIAGIL
                     ARMDGPILWVLRQADLFAPGLAGVGLNPDRIVFAEAGKQVLAVMEEGLRHSGLAAVVA
                     EHTGRLSLVASRRLQLAAEQTGVLAILVRRSRSFEDPVLNEPTAAVTRWRIAALPSPP
                     ALPHAPDTPGLGRVRWQLDLTRCRGGEPGSWVVEACDATGHLGLVSDTAKRSIKAA"
     gene            2174..3478
                     /locus_tag="VGC82_05235"
     CDS             2174..3478
                     /locus_tag="VGC82_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEY0422709.1"
                     /translation="MPAPFDPINLRRLAEWCLRYSPLTAPDPPDGIWIDVTGGIHLHG
                     GEARLLRDLLQRLNGQGLSAQALSPRAAVADTPALAHAVARFGGGGVVPPGAVVSYAA
                     VMADFPIEALRLPPDTLAGLRLMGFERVGPLAAAARAPLVRRFGPLLAVRLDQAAGIL
                     FEPIMPVTPPASIQARLTFVEPLLTAEAFSTVITRLVVLVCGDMERAGLGARRLELLF
                     ERVDRSVQAIHIGTARPARDTRHLGRMLDERLERVDPGPGVEAMHLIVAAADPLGFIQ
                     TSSSLLADASPDIAPLVDRLANRLGEAMVYRIAPVESDVPERSVRLMPALTRITGTWP
                     ADLPRPVRLLDPPQPVDAMALLPDHPPVTFIWRRVRHRVRHADGPERIAGEWWKRDRE
                     WFAVRDYFRVEDEDGRRFWLFRRGNGTDTETGDMRWFLHGFF"
     gene            3943..>6655
                     /locus_tag="VGC82_05240"
     CDS             3943..>6655
                     /locus_tag="VGC82_05240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEY0422710.1"
                     /translation="MTGRHARYTELQVTTNYSFLRGASHIEELMTRAAVLRMDALAIT
                     DRNSLAGIVRAHQRAREAGIRLIVGCRLDLKDGPSVLVYPTSRDGYSRLTRLLTLGKG
                     RAGKGKCELYWPDLAATSEDLIAILCTDPTEANLCRLKTDFKNRAYVALTLMHRPNDA
                     VRLRTIADTAHALGVPAVAANDVLYHAPGRRILQDVLTCIREGCTIDELGYRRERSTV
                     RHLQPQDEMARLFVRHPDAVARTQEIADRCRFSLDELRYQYPHEVRIPGLTAQQTLER
                     RTWESAATRYPDGIPEKVLRQLHHELDLIAGLDYAPYFLTVDSIVRFARSQHILCQGR
                     GSAANSAVCFVLGITSIDPTRSDLLFERFVSAERKEPPDIDVDFEHERREEVIQWIYR
                     TYGRHRAALCATIIRYRGRGAVRDVGKALGLTEDVTAALASQVWSWSEEGVSEEHAVA
                     LNLDLENRRLRLTLDLAKTLMGFPRHMSQHPGGFVLTEERLDDLVPIEPASMANRQVI
                     EWDKDDIDILRFMKVDVLGLGMLGCMRRAFDLLADHKGQAMDLAAIPAEDPATYAMIR
                     KADTLGTFQIESRAQMSMLPRLKPRTFYDIVIQVAIVRPGPIQGDMVHPYLRRRDGLE
                     KVEYPTVELRRVLEKTLGVPLFQEQAMQVAIVCAGFTPSEADALRRSMATFKFTGGVS
                     HFKEKLVAGMIDRGYTAEYADKTFSQIEGFGSYGFPESHAASFALIAYASAWMKCHHP
                     DVFCCALLNAQPMGFYATAQIVRDAQLHGVEVRPVSVNESAWDCTLEPHDSDGERYAV
                     RLGLRMAKGLASLHADEMIARRGTGYRSVEDMWRRLHVPVAALERLAEADAFRTLGLD
                     RRQALWAIRGLSDTRLPLFDTVPAEPDTEPAVALAAMTAGRQVVEDYR"
BASE COUNT         1245 a         2128 c         2056 g         1226 t
ORIGIN      
        1 tgggcggagc gcaacccggc cggccgcaca gcagcgcggc accgagcaac aggcgcacga
       61 catagcgccc atgtgcatga ttcatcgccc gagcggtggt tgcctgagcg gtggttgcct
      121 gagcggtggt caatcgagac atgatgtttt cccgcacgcc taataaatat cgatatcgaa
      181 aatatatcta tatccaacta gttaatcaat tgttgatatt atccggccat gccccggcaa
      241 atggtcatcg aggcgaacgt ttagcttcgc ttcaatgccc ccaaacaatc agcaggacgc
      301 ctccgaccac cagcaccaga cccgcgactt cacctttttt taattgctcc ttcagataga
      361 atcggctgaa cagcagggtg aaaacgatct cgatctgccc aactgaacga accaggccaa
      421 cgtcggccat ggcgaaagcg gtgaaccagc aggcggatcc gcatcccgac agcgttcctg
      481 cccacataga cgtgcgccaa gtggtgaagg ctttgcgcaa ctccaccggc tcccgccata
      541 acagccaggc gccttgcatc aagatctgca acatattcgt cagcagcacg gcgaacagcg
      601 ccttcgcaac caggcttggc ccgggaatcg actggttcgc caacttgatg aaaatggtgg
      661 taaaagcgaa caggaacccg gctcccagcc cgcataaagc cgccggctgc acgatcgcgg
      721 ccagcagttc acgcggtttg atcccacgcc ccgccaccga cagcgtcatc actccggaca
      781 gccccagcgc gatgccgatc caggacagcg gcttgaggac ctcgtgaagg atgatcagtg
      841 cgaccaccgc gctttgcgcc gcctcggtct tgccgtaagc ggtgccgacg gcaaaattac
      901 ggtagccgaa ggacatgatc agcaggttgg ttgccaggat ctggagcagc ccgccggcaa
      961 cacagtacaa caggaaacgg aggttcggca ccggcaccgg ggcagccgtg atccacagcc
     1021 cagcgagaaa aagcagcagc gccgttgggg cgccatacag gaagcgcacg aagccggcac
     1081 cgttgaccga cagcaaatgg cgcagcttct gctgcaatgc ggtgcgccaa cactgaaaca
     1141 aggccgcggc gatggtgatg ggaatccatg ttaccatcgc ggcgtctcct tattttgtga
     1201 ctatccaacc ggcggagacg aagcctgtca atccgccaaa cagcattcac aatgacgcca
     1261 aagtctcgat cataagactc gacttggccg ttggtcgggt tttatgttca tgttatgttc
     1321 actactcttg ccattcccaa aaagccgtcg ctgagtgggc attctgctgc cggaccagcc
     1381 ccacgcactc cgcctgtagc tgccccgtcg ccagctatcg ccgcgctgcg ggaacgcatt
     1441 ggccggattg aacgccttca cgcgcgcggc cctgccgaac gatccgttcc tttgacgatc
     1501 gaggcgatcg accaggcgct gccgaccggc ggcatccgcc ttggtgcgct acatgaagct
     1561 gcctcggccg ggccggacac ggaacacgcc gctgccgcca ccttgttcat cgccggtatt
     1621 ctggcacgta tggatgggcc catcctatgg gtgctgcggc aggcggatct gttcgccccc
     1681 ggccttgccg gcgttggact gaacccggac cggatcgttt tcgccgaggc gggcaagcag
     1741 gtgctggccg tcatggaaga aggcctgcgc cattccggcc ttgccgccgt ggtggctgag
     1801 cataccgggc ggctcagtct tgtcgcctcg cgccggctgc aactcgcggc ggagcagact
     1861 ggggtcctgg ctatcctggt ccgccgtagt cgaagcttcg aagatccggt gctgaacgag
     1921 ccaaccgctg ccgtcacgcg ttggcggatc gccgccttgc cctcgccgcc ggctttgccg
     1981 cacgcgccgg atacaccggg cctggggcgg gttcgctggc agctggacct gacaagatgc
     2041 cgcggcggag agccgggatc ctgggtagtg gaggcttgcg atgcgacggg tcatctcggt
     2101 ctggtttccg acaccgccaa acgatccatc aaggcagcct gacgaccaag gctctgccct
     2161 ggacccgcaa aggatgcctg ccccctttga tcccatcaac ttaaggaggc tggcggaatg
     2221 gtgcctgcgc tattcaccgc tgaccgcgcc cgaccctcct gacggcatat ggatcgatgt
     2281 caccggcggc attcatctgc atggtggaga agccagactc cttcgcgacc tgttgcagcg
     2341 cctgaacggc cagggattgt ccgcccaagc cctgtctccc cgggccgccg tcgccgacac
     2401 ccctgccctc gcccatgccg tcgccaggtt tggcggcggc ggcgtagtgc cccctggtgc
     2461 ggtagtctcc tatgcggcgg tcatggcgga ttttccgatc gaggcattgc gcttgccgcc
     2521 cgacacactg gccggtctcc ggctaatggg attcgagcgg gtcggcccgc tggccgccgc
     2581 cgcccgcgcc cccctggtcc ggcggttcgg cccgttgctg gcggtcagac tggatcaggc
     2641 ggcgggcatc ctgttcgagc cgatcatgcc cgtgacgccg cccgcatcga tccaggccag
     2701 actgaccttt gtcgagcctt tgctgacggc ggaagccttt tccacggtca tcactcgtct
     2761 ggtcgtgttg gtctgcggcg acatggagcg agccggcctc ggcgcccggc ggctggagct
     2821 gctgttcgaa cgcgtggata ggtccgttca ggccattcac atcggaacag ctcggccggc
     2881 ccgcgatacg cgtcacctcg gccgcatgct ggatgagcgg ctggaacgcg tcgatcccgg
     2941 tccgggcgta gaggcgatgc acctgatagt cgccgccgcc gatcccttgg gtttcattca
     3001 aacctcatcg tctttgctgg cggatgcatc gcctgacatc gccccgctgg tcgatcgctt
     3061 ggccaaccgg ctgggggagg cgatggtgta tcgtatcgca ccggtggaga gcgacgtacc
     3121 ggaacgatcg gtgcgcctga tgccggcatt gacgcgcatc accggcacct ggccggccga
     3181 tctgccccgg ccggtgcgac tactcgatcc accacagccg gtcgatgcga tggcattgtt
     3241 gccggatcac ccgccggtca ccttcatttg gcggcgcgtg cgccaccggg tgcgccacgc
     3301 cgatggaccg gaacgcatcg ccggggagtg gtggaagcgc gaccgcgaat ggtttgccgt
     3361 gcgcgactat ttccgggtgg aggacgaaga tggccgccgc ttctggctgt tccgccgcgg
     3421 caatggcacc gacaccgaaa ccggcgatat gcgctggttc ctgcacgggt ttttctgatg
     3481 gggagaagtg gaggttaagg ccaccatgaa catgctgaga attctgcctt tagcaagtaa
     3541 gcaaattttt aacactgaga aagaacgagg atcacgaagg gccacggaga aaataaggct
     3601 ggcgcttcgt gcaaagtgcg ctcaagctac gctgccaaag cgaattaaat ttaagttgta
     3661 ttgttactat ataataattt ttggcggccg tccaactgga tgcctggtac ttatctcacg
     3721 tcatggtggc cgaagggtta ccatccacga ctttggtagt acgagaaggc gtggatgccg
     3781 gtcttcgccg gcaagacgag gtggcgccgt taaacagtcg gtctcaacgc agattggtat
     3841 tgataccact tacttcctcc gtggcccttc gcggccctcg ttcttccttc gtgttaaaaa
     3901 ctttctcgcg gcctcaacgc ccgtccaagc ggacgttccc ccatgacggg acgacacgca
     3961 agatataccg aactccaggt caccacgaac tacagcttcc tgcgcggcgc ctcgcatatc
     4021 gaggaactga tgacccgcgc cgccgtcctg cgcatggacg cgctggctat caccgaccgg
     4081 aactccctgg ccggcatcgt ccgagcgcac cagcgtgctc gggaagctgg gattcgcctg
     4141 atcgtgggct gccggctgga cctgaaggac ggaccctcgg tgctggtcta tccaaccagc
     4201 cgagatgggt attctcgcct gacccgcctg ctgacactgg gaaagggccg cgccggcaag
     4261 ggcaaatgcg aactttactg gcccgatctg gcagcgacat cggaagacct gatcgccatc
     4321 ctctgcaccg accccaccga ggcaaacctg tgtcgcctga aaacggattt caaaaaccga
     4381 gcctatgttg ccttgacctt gatgcaccgt cccaacgacg cggttcggct gcgtaccatc
     4441 gccgacacgg cccatgcgct gggcgtgccg gccgtcgccg ccaacgacgt gctgtaccat
     4501 gcgcccggcc ggcgcatcct gcaggatgtg ctgacctgca ttcgcgaagg ctgcaccatc
     4561 gacgaactcg gctaccgccg cgagcgttcc actgtgcggc atcttcagcc acaagatgaa
     4621 atggcccgcc tgttcgtccg ccaccccgac gccgtggccc gcacgcaaga gatcgccgat
     4681 cgttgccggt tcagcctgga cgaactccgc tatcagtatc cacacgaggt ccgcattccc
     4741 ggactgacgg cgcaacaaac cctggagcgg cgaacctggg aaagtgccgc cacacgatat
     4801 ccggacggca ttcccgaaaa ggtcctgcgg cagcttcacc atgagctcga cctgatcgcc
     4861 ggcctggatt acgcacccta cttcctgacg gtggacagca tcgtccggtt cgcccgctcc
     4921 caacatatcc tctgccaggg ccgcggatcg gcggcgaact cggcggtgtg tttcgtgctc
     4981 gggatcacct ccatcgatcc cacccgcagc gacctgctgt tcgaacgttt cgtcagtgcc
     5041 gaacgcaagg aaccccccga tatcgatgtc gatttcgaac acgagcggcg cgaggaagtg
     5101 atccagtgga tctaccgcac ctatggccgt catcgcgccg ccttgtgcgc gactatcatc
     5161 cgctaccgtg gccgcggcgc cgtccgtgac gtcggcaagg cacttggcct gaccgaggac
     5221 gtgaccgctg ccctagcctc ccaggtctgg agctggagtg aggaaggtgt gtccgaggaa
     5281 cacgccgtgg cgctgaacct tgatctggaa aaccgccggc tgcgtctgac cctggatctt
     5341 gcaaagacgt tgatgggatt tccgcggcac atgtcgcagc accccggtgg tttcgtcctg
     5401 accgaggaac ggctggacga tctggtgccg atcgagccgg ccagcatggc caaccgccag
     5461 gtgatcgagt gggacaagga cgacatcgac atcctgcgct tcatgaaggt cgatgtgctg
     5521 gggctgggca tgctgggctg catgcgccgc gcgttcgacc tgctggcgga ccacaagggc
     5581 caggcaatgg atctggcggc gatcccggcc gaggatccgg cgacctacgc gatgatccgc
     5641 aaggccgaca cgctgggcac gttccagatc gaatcccggg cacagatgtc gatgctgccc
     5701 cggctgaagc cacgcacatt ttacgacatc gtcatccagg tggcgatcgt ccgtcccggg
     5761 ccgatccagg gcgacatggt gcatccctat ctgcgccgcc gtgacgggct ggagaaggtg
     5821 gagtacccaa cggtcgaact acgccgcgtg ctggaaaaaa ccctgggcgt gccgctgttt
     5881 caggaacagg cgatgcaggt ggcgatcgtc tgcgccggct tcacgccgtc cgaggccgat
     5941 gcgctccgcc gcagcatggc gacattcaag ttcactggcg gagtgtcgca tttcaaggaa
     6001 aagctggtcg caggcatgat cgatcgcggc tacaccgccg aatacgccga caagacattc
     6061 tcccagatcg agggtttcgg cagctacggc tttccggaaa gccatgcggc gagtttcgcg
     6121 ctgatcgcat atgcctcggc atggatgaaa tgccaccatc ccgatgtgtt ctgctgcgca
     6181 ctgctgaatg cccagccgat gggcttctac gccacggcgc agatcgtgcg cgatgcccag
     6241 ttgcatggcg tggaggtgcg gccggtttca gtgaacgagt cggcgtggga ctgcaccctg
     6301 gagccgcatg acagcgatgg cgaacgctat gcggtgcggc tgggattgcg gatggccaag
     6361 gggcttgcat cgctccatgc cgacgaaatg attgcccgtc gcgggaccgg atatcgttcg
     6421 gtcgaggata tgtggcggcg gctgcacgtg ccggttgcgg cactggagcg gctggccgag
     6481 gcggatgcgt tccggacgct ggggctggac cggcggcagg cgctgtgggc aatccgcggg
     6541 ctgtcggaca ccaggctgcc gctgttcgac actgtccccg ccgaaccgga tacggaaccc
     6601 gccgtggcat tggcggcgat gaccgccggc cggcaggtgg tggaggatta tcgca
//
LOCUS       DASWQN010000317         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_295388, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000317 DASWQN010000000
VERSION     DASWQN010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295388"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1643)
                     /locus_tag="VGC82_05245"
     CDS             complement(<1..1643)
                     /locus_tag="VGC82_05245"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEY0422711.1"
                     /translation="MYIEAVPNRNSPPAILLRESYRENGKVHKRTLCNLSNWPVAHVE
                     GLRGVLKGGTVIPAERDAFTVTRSLPHGHVAAALGTARKIGLDGILGPDGNRCRDLVL
                     AMLVSRILDPVSKLATARALSPDTAASSLGEALGLGAVDDDELYAALDWLLERQPGIE
                     TILAKRHLKNGTLVLYDVSSSYMEGSHCALAKRGYSRDGKKGTLQIVYGLLCAPDGCP
                     VAIEVFDGNTGDPSTLALQIEKLKQRFHLNHVVLVGDRGMITDARITEDIKSAGLDWI
                     TSLRAPAIKDLLNSGTLQLTLFDQRDVASITSPEFPGERLVVCRNPDLAAERARKRED
                     LLTATEKDLTRIKAAVARKRDPLRGTAEIALAVGAVLNTHKMKKHFDLELTDTAFSFT
                     RKTEAIAAEAATDGIYVVRTSLAEATLGDIDTVRSYKSLSLVERAFRCIKTVDLHVRP
                     VYHWLEGRVRAHVFLCMLAYYLEWHMRQRLAPMLYDDTDKEEAEAQRRSVVAQAQRSK
                     AAVKKQTTGMTPDGLPVHSLRSLLADLATLARNTITTAIT"
BASE COUNT          330 a          527 c          641 g          365 t
ORIGIN      
        1 gtgatggcgg tggtgatggt gtttcgcgcc agcgtcgcca gatcggccag cagcgagcgg
       61 agactgtgca ccggcaatcc gtccggggtc atgccagtgg tctgcttctt gaccgcggcc
      121 ttggaacgct gcgcctgcgc cacgacactg cgtcgctgcg cttcggcttc ttctttgtcg
      181 gtatcgtcat acagcattgg ggccaggcgc tgacgcatgt gccattccaa ataataggcc
      241 agcatgcaga gaaacacatg cgcgcggacc cggccctcca gccagtgata gaccggccgg
      301 acatgcagat cgacggtctt gatgcaacga aacgcccgct ccaccaggct caaggatttg
      361 tagctacgta ctgtatcgat gtcaccgagc gtcgcctcgg ccaggctggt gcgcacgaca
      421 tagatgccgt cggttgccgc ctcggcggca atggcctcgg tcttgcgggt gaagctgaaa
      481 gcggtgtcgg tgagctccag atcgaaatgc tttttcattt tgtgcgtgtt gagcaccgcg
      541 ccgaccgcaa gcgcgatctc ggcggtgccg cgcagtggat cacgcttgcg cgcaacagcg
      601 gccttgatgc gggtgaggtc cttctcggtc gccgtcagca ggtcctcgcg cttgcgggcg
      661 cgctcggcgg cgagatcggg gttgcggcac accaccagcc gctcgccggg gaattcgggg
      721 gaggtgatgg aggccacgtc gcgctggtcg aacagcgtga gctgcaaggt gccgctgttc
      781 agcaggtcct tgatcgccgg cgcgcgtaac gaagtgatcc agtccaggcc tgcggatttg
      841 atgtcctcgg tgatgcgggc atcggtgatc atgccgcggt ccccgaccag cactacatgg
      901 ttgaggtgga agcgctgctt cagcttctcg atctgcagtg ccagggtcga aggatcgccg
      961 gtgttgccgt cgaacacctc gattgcgacg ggacagccgt ccggggcaca aagcaaaccg
     1021 tagacgattt gcaacgtgcc cttcttgccg tcacggctgt agccgcgttt ggcaagtgcg
     1081 cagtggcttc cttccatgta gctcgacgag acatcgtaga gcaccagggt gccgttcttg
     1141 agatgacgtt tggccagaat ggtttcgatg ccaggctggc gttccagcag ccagtcgagc
     1201 gcggcataga gttcgtcgtc atcgaccgcg ccgagcccga gcgcttcgcc gaggctcgat
     1261 gcggcggtgt cgggggagag ggcgcgagcg gtggcaagtt tcgagacagg atcgaggatg
     1321 cgactgacca gcatggccag gacgaggtcg cggcagcgat tgccgtcggg gccgaggatg
     1381 ccgtcgagac cgatcttgcg cgcggtgccg agggcggcgg cgacatggcc gtgcggcagg
     1441 gagcgggtga cggtgaaggc atcgcgttcg gcgggaatga cggtgccgcc cttgagcacg
     1501 ccgcgcaggc cttcgacgtg ggccacgggc cagttggaga ggttgcacag ggtgcgctta
     1561 tggaccttgc cgttttcccg atagctctcg cgcagcagaa tggcgggggg cgagttgcgg
     1621 ttcggaacgg cttcgatata catggccgga aaggagtcga ttattcctcc tggtacaaat
     1681 cacaatccag tgattacatg ggcacgtttc aatggccaca gtgcgacttt cccaggatca
     1741 cggccggatt catagtatcg gcaagaggga agctggggat actccaggaa tgtgaacaaa
     1801 ctgtttcgca ctttgccgta acgttttcgc agccgctggc catgcggatt ggttggagca
     1861 agc
//
LOCUS       DASWQN010000318         3468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_163529, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000318 DASWQN010000000
VERSION     DASWQN010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163529"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            358..1518
                     /gene="recF"
                     /locus_tag="VGC82_05250"
     CDS             358..1518
                     /gene="recF"
                     /locus_tag="VGC82_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010501578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEY0422712.1"
                     /translation="MLRLTRLTLTDFRSYAGLTWRPVTRIAVLHGPNGSGKTNLLEAI
                     SLLVPGRGLRNARNADLPRQDGPGGWAVAARLRTVDGETVLGTGSTDQADRRVFRLDG
                     AAPRSQSDIARLVAAVWLTPQMDRLFQEGLSGRRRFLDRLVWSLEPGHAREMAAHDAA
                     VASRNRLLAEGRLDRSWLAGLEDSIARHAVAASAARLGLTSSLNAQITANTGAFPPAR
                     IDLACQIAERLQTEPALAVEDWLRAGLAANRARDAEAGTSALGAHRTDMRLTDVTTGT
                     SAALASTGQQKALLIGVVLAHAGLIAASRGFAPLLLLDEPAVHLDADRRRALWRVLSS
                     LPAQTVITGTDADAFLPLAAQAEGFAIRDSVLYPDERFLRPDIEASPVTGRR"
     gene            1698..>3468
                     /locus_tag="VGC82_05255"
     CDS             1698..>3468
                     /locus_tag="VGC82_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEY0422713.1"
                     /translation="MSENAAPPPDSDPDAYDAASISVLKGLDAVRRRPGMYIGDTDDG
                     SGLHHMAFEIIDNAVDEVQAGFASHVELTLNGDGSVTVRDDGRGIPTDMHVEEGVSAA
                     EVVLTRLHAGGKFNQNSYKVSGGLHGVGAAVVNALSEWMEVRIWRLGQEHRIRFQHGE
                     PDGPLEIVGQADRGSGTEVTFKASPGTFTKTEFDYTLLERRLRELAFLNSGMTVVLRD
                     ERHAPALESVMRYDGGLVAFVEWLDRSKNAVFTPPISLRTTADQQGIRAEFALSWNDS
                     FHETMLCFTNNIPQRDGGSHLAGFRQALTRVVTKYAQGMGKKDSLELVGEDMREGMTA
                     VLSVKVPDPKFSSQTKDKLVSSEVQPVVQAAVADAVSHWFETHPKEAKGIIQKVMDAA
                     AAREAARKARELTRRKGVLDISTLPGKLADCQERDPAKCEIFIVEGDSAGGSAKQGRD
                     RKFQAILPLKGKILNVERARFDRMLGSQEIGTLITALGTGIGPGAVEQGGFDAGKLRY
                     HRIVIMTDADVDGSHIRTLLLTFFFRQMRELIERGHLYIAQPPLYRAKRGSDERYLKD
                     DNALESFLLDKALADARLTYANGD"
BASE COUNT          621 a         1131 c         1117 g          599 t
ORIGIN      
        1 gaactccgta cgcgaggata attcggccgt gtggcacccg ccttcgatga aacctaacgc
       61 gcgaagcggc ctcggtccca gaagcttcgg tcccagaagc ctcggtccca gaagccccgg
      121 tccagaagcc tcggtcccgg aagccttggt cccggaagcc ttggtcaatg caacacgcag
      181 caggcctcga ctcgtggtgc cgcgaccttc cgcgtgaacc gtggtgagtc cgcataatcc
      241 gtgatgaacg gtttactgcg tccgcagatt cctgatgaac gatttattgc atccccagct
      301 tcacccgccc gacgatgatc gcttcacggc aaggcatgat gcgaccatct tcccaccgtg
      361 ctccgcctga cccgcctgac gttgaccgat ttccgcagct acgccggcct cacctggcgg
      421 ccggtgacgc ggatcgccgt gctacatggc ccaaacggca gcggcaagac gaacctgctg
      481 gaagcgatat cgctgctcgt cccgggccgc ggcctgcgca acgcccgcaa tgccgatctg
      541 ccacgccagg atggtcccgg cggatgggcg gtcgccgctc gattgcgaac ggtggatggg
      601 gaaaccgtcc ttggtaccgg ctcgaccgac caggcggatc gccgcgtgtt ccgtttggat
      661 ggtgccgcgc cacgcagcca gtcagacatc gcccgcctgg tcgccgccgt ctggctgacc
      721 ccgcagatgg accggctgtt ccaggagggg ctgtccgggc gccggcgctt cctcgaccgg
      781 ctggtctggt cattggaacc cggacacgcg cgggagatgg ccgcccatga cgccgccgtg
      841 gcaagccgaa atcgcctgct ggcggaggga cggctcgacc ggtcctggtt ggccggcctg
      901 gaggattcga tcgctcgcca cgccgttgcc gcctccgccg cccgcctcgg actgacatcc
      961 agcttgaacg cccaaatcac ggcgaacacc ggcgccttcc ccccggcccg catcgatttg
     1021 gcctgccaga tcgccgaacg gctgcagacc gaaccggcct tggcggtgga ggactggctg
     1081 cgcgccggcc tggccgccaa tcgcgcccgg gatgcggagg ccggcaccag tgcgctgggc
     1141 gcccaccgga ccgacatgcg cctgacggac gtgaccaccg gcacgtcggc tgccttggcg
     1201 agcaccgggc agcagaaggc gctgctgatt ggcgtggtcc tggcccatgc cgggttgatc
     1261 gccgcttcgc gcggcttcgc ccccctgctg ctgctggacg aacccgccgt gcatctggac
     1321 gcagaccggc gccgggcgct gtggagggtg ctgtcgtcac tgccggcgca gacggtgatc
     1381 accggcaccg atgccgacgc cttcctacca ctggcggcgc aagccgaggg cttcgccatc
     1441 cgcgacagcg tgctgtatcc ggatgagcgt tttcttcgtc ccgatatcga agcttcccct
     1501 gtcaccgggc gccgctagat gctatctatc tagagtgtcc tggccccgaa gttggtcgca
     1561 gtttgcgacc gacgcagggg atgagcaggt cactgaaaac aaagagcgat tgtcctgatt
     1621 caaacgttca ctcactaggg agaacgtttg aatcaggaca agagtagcaa caccaccgca
     1681 tggagtcctt taccggcatg tctgaaaacg ccgcgccgcc gcctgattcc gatcccgacg
     1741 cttacgatgc cgcttcgatt tccgtcctta aaggcctgga tgccgtccgc cggcgcccgg
     1801 gcatgtatat cggtgacacc gatgacggat ccggcttgca ccatatggcg tttgaaatca
     1861 tcgataacgc ggtggacgag gttcaggcgg gattcgcctc gcatgtggaa ctgaccctga
     1921 acggcgacgg cagcgtcacg gtacgcgatg acggtcgcgg tatcccaacc gacatgcatg
     1981 tcgaggaagg cgtctcggcc gccgaagtcg tgctgacccg cctgcatgcg ggcggaaagt
     2041 tcaaccagaa ctcctacaag gtgtcgggcg gactgcatgg cgttggcgcc gcggtggtga
     2101 acgccttgtc cgaatggatg gaggtccgta tctggcgcct gggccaggag caccgcatcc
     2161 gcttccagca tggtgagccc gatggaccgc tggaaatcgt cggtcaggcc gatcgcggca
     2221 gcggcaccga agtgacgttc aaggccagcc ctggcacctt caccaagacc gaattcgact
     2281 acaccctgct cgagcgccgg ttgcgcgaac ttgccttcct gaactccggg atgacggtcg
     2341 tgctgcgcga cgagcgtcac gcgccggcgc ttgaaagcgt catgcgctac gacggcggac
     2401 tggtcgcgtt tgtggaatgg ttggaccgtt cgaagaacgc tgtcttcacg ccgcctatca
     2461 gtttgcgcac gaccgccgac caacagggta tccgagccga gttcgcgttg tcgtggaatg
     2521 acagcttcca cgagacgatg ttgtgcttca ccaacaatat tccgcagcgc gacggtgggt
     2581 cccatctcgc cggatttcgc caggcgctga cccgcgtcgt gacgaaatac gcccagggca
     2641 tggggaagaa ggattcgttg gagctggtcg gcgaagatat gcgcgaaggc atgaccgccg
     2701 tgctgtcggt caaggtgccg gacccgaagt tttcctcgca gaccaaggac aagctggtct
     2761 cctcggaggt ccagccggtg gtgcaggcag ccgtggccga tgcggtttcg cactggttcg
     2821 agacgcatcc caaggaagcc aaaggcatca tccagaaggt gatggacgcc gccgccgcgc
     2881 gcgaagccgc ccgcaaggcg cgcgaactga cgcggcgcaa gggcgtgctg gatatctcga
     2941 cgctgcccgg caagctggcg gactgtcagg aacgcgatcc ggccaagtgc gaaattttca
     3001 tcgtcgaggg tgacagtgcg ggcgggtccg ccaaacaggg ccgcgaccgc aagttccagg
     3061 caattctgcc gctgaagggc aagatcctca acgtcgagcg ggctcggttc gatcgcatgc
     3121 ttggcagcca ggaaatcggc acgctgatca ccgcgctggg caccggcatc gggcccggcg
     3181 ccgtcgagca aggcggtttc gacgccggca agctgcgcta ccaccggatt gtcatcatga
     3241 cggacgccga cgtcgatggc tcgcatatcc gcacgctgct gctgacgttc ttcttccgcc
     3301 agatgcgcga actgatcgag cgggggcatc tgtatatcgc tcagccgcca ttgtaccgcg
     3361 ccaagcgggg ctccgacgag cgctacctga aggacgacaa tgcgctggaa tcgttcctgc
     3421 tggacaaggc gctggcggac gccaggttga cctatgccaa cggcgacg
//
LOCUS       DASWQN010000319         3701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1879170, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000319 DASWQN010000000
VERSION     DASWQN010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879170"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VGC82_05260"
     CDS             complement(<1..841)
                     /locus_tag="VGC82_05260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0422714.1"
                     /translation="MSNLALEVVGMLGQTIAKVVPITIVLAVVFSVLTHFWACNPGTA
                     WWRKRELVTDICYWFFVPLFARVLRIGLLVLGAGVVFNIHDADGLIAFYDNGHGPLSE
                     LPLWLQAMLFLVLSDFMLYWLHRMFHGGAFWKYHAIHHSSEDLDWISAARFHPVNLFL
                     GTIMVDVILLMAGISPNIMLWVGPFTMFHSAFVHANLSWTLGPFKYVLATPVFHRWHH
                     TPFEHGGNTNFAGTFPVWDILFGTFRMPENKLPDGYGADDPAIPSEIAGQLAYPFRQI
                     GSEL"
     gene            1141..1305
                     /locus_tag="VGC82_05265"
     CDS             1141..1305
                     /locus_tag="VGC82_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY0422715.1"
                     /translation="MKNLIARFVKDESGATAIEYGLIAAGISLAIIAVVNGLGTNLNA
                     KFTSINTSLK"
     gene            1379..1525
                     /locus_tag="VGC82_05270"
     CDS             1379..1525
                     /locus_tag="VGC82_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422716.1"
                     /translation="MRKPGAASIAARKPSAPPGSRSDTPIELICAAMALALLVLVARI
                     FSVW"
     gene            1617..2141
                     /locus_tag="VGC82_05275"
     CDS             1617..2141
                     /locus_tag="VGC82_05275"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY0422717.1"
                     /translation="MILDIARLLLFPALMAFAAASDLFTMTISNRVSLALAAGFLALA
                     PFGGMGLYEVAAHLGAGATVLAVAFGCFAMGWIGGGDAKVAAAAALWFGFGHLLDYLL
                     YASLFGGGLTLLLLQFRQWPLPYLFAGQAWLLRLHAKESGIPYGIALAVGALMIYPET
                     EWIKAIDLTHFAIH"
     gene            2462..>3701
                     /locus_tag="VGC82_05280"
     CDS             2462..>3701
                     /locus_tag="VGC82_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEY0422718.1"
                     /translation="MSCRLNQRSLRALMVRALSFSAAAALTLNPALTPVVASDYRAAV
                     PVVAADGQMNARFLSLGIGKSVVIDLPRDIKDVLVADPKIATAVIRSAQRAYIIGAAV
                     GQTNIVFFDSAGQQIAAYDIAVKRDLNGVRAALKQTLPNADILIEGIGDGVVLSGTAA
                     SPIEAQQAGDLAGRLVGGADKVINSIAVRGRDQVMLKVTVAEVARSIIKQLGIDLSAS
                     LNYGTSVVNFNNSNPFTAYGRSLVSNNNMSASFGATPSVQATLRAMESAGVVRTLAEP
                     NLTAISGESATFIAGGEFPIPAGYACDPVTHVCTTQISYKKFGISLNFTPVVLTEGRI
                     SLRVMTEVSELSNENGITLNQAGNSLTVPSVKTRRAETTLEIPSGGAMAMAGLIQEQT
                     KQAVNGLPGLDQLPVLGALFR"
BASE COUNT          701 a         1151 c         1133 g          716 t
ORIGIN      
        1 ccagttcgga tcctatttgc cgaaacgggt aggccagttg gccggcgatc tcggacggga
       61 tcgctggatc atccgcgccg tagccatcgg gaagcttgtt ttccggcatc cggaaggtcc
      121 cgaacagaat gtcccaaacc gggaacgtcc cggcaaagtt ggtattgccg ccatgttcga
      181 agggcgtgtg gtgccagcgg tggaacaccg gcgtcgccag cacgtatttg aacgggccaa
      241 gcgtccagct gagattggcg tgaacgaacg ccgaatggaa catcgtgaag ggcccgaccc
      301 acagcatgat gtttggcgag attcccgcca tcagcaagat cacgtcgacc atgatcgttc
      361 ccaggaacag gttcaccggg tggaagcggg cggcggaaat ccagtccaga tcttccgacg
      421 aatgatggat cgcgtgatat ttccagaagg cgccgccgtg aaacatgcga tgcagccagt
      481 acagcatgaa gtccgacaaa accaggaaca gcattgcctg aagccaaagc ggcagttcgg
      541 acagaggccc atggccgttg tcgtagaagg caatcagtcc atcggcatcg tgaatgttga
      601 agacaacgcc ggcgccgagc acgagcagcc cgatccgcag cacgcgcgcg aacaatggaa
      661 cgaagaacca gtagcatatg tcggtgacga gctcgcgttt gcgccaccag gccgttccgg
      721 gattgcaggc ccagaagtga gtcagcaccg agaacacaac ggccagcacg atcgtgatgg
      781 gcaccacctt ggcgatggtc tggccgagca tcccgacgac ttcgagggct agattggaca
      841 tggccgcctc atctgatcag gtctctaggc gtatcgcttc gtgcttaacg agccgtgaac
      901 cggggccggc gcgcacttgt cgtcccccgc cgggcgcctg tatccattct gcattgtatc
      961 ggcgggctta attccaaatt tactctgctc aacaacagcc tgctaactgc aagtttgaat
     1021 gttcaaatta accgcgccgc aagctaccga ccgttacggt tcggtatggt cacggtgctg
     1081 agaacgccgg ggagaccaca cgtttagttc gaacagccac gtgtacagat ggagccatct
     1141 atgaagaatc tcattgcgcg tttcgtgaag gacgagtccg gtgcgaccgc gatcgaatac
     1201 ggcctgattg cggccggcat ttcgctcgcg atcatcgctg tggtcaacgg cctcggcacc
     1261 aacctgaacg ccaagttcac ctcgatcaac acctcgctga aataagcgac tgtagagcga
     1321 gatcaaaggc cccggcgatc cggggccttt ttttcggccg cacggtggtg gcataaatat
     1381 gcgcaagccc ggcgccgctt cgatcgcggc aagaaagccc tccgcgccgc ccggcagccg
     1441 gagcgatacg ccgatagaac tgatctgtgc cgcgatggcg ctggcgctcc tcgttctcgt
     1501 cgcccggatt ttcagtgtct ggtgatcggt cggactattt tccgattgtt catatctcgc
     1561 cactaggctg cggcgatgct ccgagcctcc ggtagcagca cgagctgctt ggtcccatga
     1621 tcctcgacat cgcgcgcctg ttgctgtttc cggccctgat ggcgttcgcg gccgcgagcg
     1681 accttttcac catgacaatt tcgaaccggg tgtcgctggc gctcgcggca ggcttcctgg
     1741 cgctcgcgcc gttcggcggc atggggcttt acgaggtcgc cgctcatctg ggtgcgggcg
     1801 cgactgtact cgcggttgcc tttggctgct ttgccatggg ctggatcggc ggtggtgatg
     1861 ccaaggtggc ggccgcagcc gcgctctggt tcggcttcgg ccatttgctg gactacctgc
     1921 tctacgcctc gctgttcggc ggcggcttga cgctgctgtt gcttcaattc cggcagtggc
     1981 cgctgcccta tttgtttgcc ggccaggcct ggctgctcag gctgcacgcc aaggaaagcg
     2041 ggatccccta cggcatcgcg ctcgcggtcg gcgccctgat gatctatccg gagaccgaat
     2101 ggatcaaggc gatcgacctg acgcacttcg ccattcactg agcgcgggct cagtaaaccc
     2161 ggcgttaagt tgatttagat acgccttctt aaccatgctt tgacgaatag ctggtcaact
     2221 ccgatccacg gcggcggcag cgtcgcggcg tcttgtggaa agtgaagcgt atgaataccg
     2281 cacgcattgt ggtcctgact ctcgctgtcg gtgccggcgg cgttgcatat cgcgtgagcg
     2341 gatctgacaa caagcctctc ccgaccgaac cggttgcgca actgcgcggc aagaccgccg
     2401 acgcggtccg ccacggcgtc tcaatctcgg cgacccggcc gtgaccgaaa gaacgcccga
     2461 gatgagctgc aggctaaatc aacggtcgtt gcgggccttg atggtccgcg ctttgtcatt
     2521 ttcggccgct gctgcgctga ccctcaatcc cgccctgacg cccgtcgttg cgagcgacta
     2581 tcgcgccgcc gtgcccgttg ttgccgccga tggccagatg aatgcgcgct tcctgtcact
     2641 cggcatcggc aagtccgtcg tgatcgacct gccgcgtgac atcaaggacg tgctggtcgc
     2701 cgatcccaag attgcgaccg ccgtgatccg ctcggcgcag cgcgcctaca tcatcggcgc
     2761 cgccgtcggc cagaccaata tcgtcttctt cgattccgca ggccagcaaa tcgccgccta
     2821 cgacatcgcg gtcaaacgcg atctcaacgg agtgcgcgcc gcgttgaagc agacgctgcc
     2881 gaatgccgac atcctgatcg agggcatcgg tgacggcgtg gtgctgtccg gcaccgcggc
     2941 gagcccgatc gaggcgcagc aggcgggcga tcttgcgggc cggctggtcg ggggagcgga
     3001 caaggtcatc aactccatcg cagtgcgcgg ccgcgaccag gtgatgctga aggtcacggt
     3061 cgccgaagtc gcccgatcga tcatcaagca gcttggcatc gacctcagcg ccagcctgaa
     3121 ttacggcacc tcggtcgtga acttcaacaa ctccaacccg ttcacggcgt atggccggtc
     3181 gctggtttca aataacaaca tgtcggcgtc gttcggcgca acgccgtcgg tccaggccac
     3241 attgcgtgcg atggaaagcg cgggcgtggt gcggaccctg gcggagccca acctgacggc
     3301 gatttcgggc gagtccgcga cattcatcgc cggcggcgaa ttcccgattc cggccggcta
     3361 tgcctgtgat ccggtcacgc atgtctgtac gacccagatc agctacaaga aattcggcat
     3421 atcgctcaat ttcacgccgg tcgtcctgac cgaggggcgg atcagcctgc gggtgatgac
     3481 cgaggtctcc gaactgtcca acgagaacgg catcacgttg aaccaggccg gcaattctct
     3541 gaccgttcct tcggtcaaga cccgccgcgc cgaaacgacg ctggaaatcc cgtcgggcgg
     3601 cgcgatggcg atggccggcc tgatccagga acagaccaag caggccgtca acggcttgcc
     3661 cgggctcgat cagttgcccg tcctcggagc gcttttccgg a
//
LOCUS       DASWQN010000320         1988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1747064, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000320 DASWQN010000000
VERSION     DASWQN010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747064"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VGC82_05285"
     CDS             <1..391
                     /locus_tag="VGC82_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422719.1"
                     /translation="SSHFATVAPNLYEMAKLGPKQIEIVQCYENFTGGVLMSLVEHGF
                     CAPEEANDLLRLENLIAPTGKMPLNTSGGNLAECYMHGLELVNEAVRQIRGVSVNQAP
                     RNDTVIVIGGPMVTPVSSLILGSEAAL"
     gene            391..825
                     /locus_tag="VGC82_05290"
     CDS             391..825
                     /locus_tag="VGC82_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY0422720.1"
                     /translation="MFYLPEGLPAPSAGADGLSTPYWEGTRREKLVIQFCRGCNHWQW
                     GPEWICHRCNSFDLDWREVAGRGIIYSYQRVWHPVHPALNGLGPYIAALVELPDAGNV
                     RMVGNLLGDPKQDVRIGAPVAAAFEHHEAGTPPFTLVHWRLT"
     gene            1057..1797
                     /locus_tag="VGC82_05295"
     CDS             1057..1797
                     /locus_tag="VGC82_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422721.1"
                     /translation="MFQAFKDTALLASVYLVGRGAHDFLHLLSSQASGCAAFRQSNGG
                     HVSLLTSIMPPLRHWMDQRRWPWPLLLSCFVLGIVPSRIAEGHGYMSEPRSRAVDHLK
                     GDIKGWPVSGAPPRYTRVDCLDLPANRQLTVVQPGPLKLGFVFPDGANHVGLCQAFLL
                     NPEHPAQKLEIGEMMDCARSDHPGPGQKKDPDIPGDMMVTIPSAVPAIRRIACCNGFG
                     WQPIAALRSRNIIMNVRISGSLARTRRS"
     gene            1794..>1988
                     /locus_tag="VGC82_05300"
     CDS             1794..>1988
                     /locus_tag="VGC82_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422722.1"
                     /translation="MTAENPIWPPRRLDQTSGASGAASSPIAEPPALAAGEDAVRQLI
                     STAMADGGAGSAQAISAIKRR"
BASE COUNT          377 a          590 c          627 g          394 t
ORIGIN      
        1 cagttcgcat ttcgccaccg tcgctccgaa tctgtatgag atggcgaagc ttggaccaaa
       61 gcagatcgaa atcgtgcagt gctacgagaa cttcaccggc ggcgtgctca tgagcctcgt
      121 ggaacacgga ttttgcgcac cggaagaagc caacgacctg ctgcggctgg agaacctgat
      181 agcgcccacc ggcaagatgc cgctgaacac cagcggcgga aacctggccg aatgctatat
      241 gcacgggctg gagctggtga atgaggcagt gcggcagatc cgcggcgttt cggtgaatca
      301 ggcgccgcga aacgacaccg tgatcgtgat cggcggcccg atggtaacgc cggtgagttc
      361 cttgatcctg ggttcggagg ccgctctgtg atgttctatc tacccgaagg cttgcccgcc
      421 ccgtcggccg gcgccgatgg gctgtccacc ccgtactggg aaggcacgcg gcgcgaaaag
      481 ctggtcatcc agttctgccg cggctgcaat cactggcagt ggggtccgga atggatctgc
      541 catcgctgca acagcttcga ccttgactgg cgcgaggtcg cgggtcgggg gatcatctac
      601 agttaccagc gcgtctggca tccggtgcat ccggccttga acggcctcgg gccgtatatc
      661 gccgcattgg tggaactgcc cgacgccggc aacgtccgca tggtgggcaa cctgctgggt
      721 gacccgaagc aggacgtcag gattggggcg cctgtggcgg cggcgttcga gcatcacgag
      781 gcaggcacgc ccccatttac gctggtgcat tggcggctga cgtagcggcc agttcgatcc
      841 gcggggaagt ttccaacgga ctgttgggcg gcggtccgcg ttgtcccaag gcttgaccgt
      901 gtggtgccgg cgtcattgat ctgagatgag ctttgggatt ccgcgatgag ttctggggtt
      961 ccgaggtaaa ttctgggccg atcgcggctt cttgcgatac tcgccgacag gagatcctcg
     1021 atcaccgcaa gatgaacgac gcccgacaat aaatccatgt tccaggcctt caaggatact
     1081 gccttattgg cctctgttta tcttgttggc cgtggcgcgc acgatttttt acatttacta
     1141 tcctcccagg cctcgggatg cgcagccttt agacagagca atggagggca tgtgagtttg
     1201 ctcaccagca ttatgccgcc cctcaggcac tggatggatc aacgtcgttg gccatggcca
     1261 ctgcttctaa gctgcttcgt gctcggtatc gtgcccagca ggatcgccga gggacacggc
     1321 tacatgtcgg agccgaggtc acgtgcggtg gatcatctga aaggcgacat caagggatgg
     1381 cccgtttcag gcgcaccgcc acggtacacg agggtcgatt gcctcgatct cccggccaac
     1441 agacagctca cggtcgttca gcccggtccc ctgaagctcg gattcgtctt ccccgacggg
     1501 gccaaccatg tcgggctgtg tcaagcgttc ctcttgaatc ctgagcatcc tgcgcagaaa
     1561 ctcgagattg gggaaatgat ggactgcgcc cgctccgacc atcccggtcc cgggcaaaag
     1621 aaagatccag acattcctgg cgatatgatg gtgaccattc cctccgcggt gcctgcgatc
     1681 cggcgcattg cgtgttgcaa tgggtttgga tggcaaccca tcgcagcatt gcgcagccgg
     1741 aatattataa tgaatgtgcg gatctcagga tcgctggcgc gcaccaggcg atcatgaccg
     1801 ccgaaaaccc aatctggccc ccgcgtcgat tggaccagac gtctggtgca tccggtgccg
     1861 cgtcgtcgcc aatcgcggag ccgcccgcgc tggcagccgg ggaagacgcg gtgcggcaac
     1921 tgattagtac tgcaatggcc gatggcggag ccggcagcgc ccaggccatc tcggccataa
     1981 aacgccgg
//
LOCUS       DASWQN010000321         1973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1879305, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000321 DASWQN010000000
VERSION     DASWQN010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879305"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..825
                     /locus_tag="VGC82_05305"
     CDS             175..825
                     /locus_tag="VGC82_05305"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0422723.1"
                     /translation="MARAEISETIPDQPPTKIAIQGQYSLPYWYDPQGKPRTFACRTI
                     TVSPFQMTLAVAVPGKIGNRINSCFPDLGNMGGSITDTFAGGFVFEPDLTDIERERMA
                     SKLVWLEKKQRDPAVRDARRHERFIPANPHSTLTLADGSTRSCFVIDMSVTGVAVSAA
                     VQPPIGAALAIGACVGRVVRLLPEGFAIQFAETQRRRDLNRLLLRVDPAPASRTGS"
     gene            complement(1102..>1973)
                     /gene="serA"
                     /locus_tag="VGC82_05310"
     CDS             complement(1102..>1973)
                     /gene="serA"
                     /locus_tag="VGC82_05310"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003628833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY0422724.1"
                     /translation="RRIFPRCVAAHQGKWDKSATNSHEVRGRTLGIVGYGNIGSQLST
                     LAEAMGMKVIYFDQTDKLRHGNTEPVESLQDLLAQSDVVSLHVPETLATSGMIGQSEL
                     RLMKRGACLINNSRGTVVDVDALAEALRDGHLAGAALDVFPVEPSSNSDHFQSPVQGL
                     TNVILTPHIGGSTEEAQDRIGKEVARKMVDYITTGSTVGAVNFPQVHLQKRQQGTRFA
                     HVHRNVPGMLRRLNEVFLERDINIVAQYLETDSEIGYVVLDVDVHGDESQGVLEDIGS
                     LDGTIRARLVYQN"
BASE COUNT          402 a          610 c          544 g          417 t
ORIGIN      
        1 gattgtattt gtagctgcct ttcaagccgg acagaagcca aagacgggaa gcctgatgcc
       61 tccgcttgtc gttgccagat ttcgagctgc tcggaacaga tcgcctgaca ccaaagtcat
      121 cacaaaatgc acggcggcaa cggttatcgc caaatgaatg ggacggaagt gttcatggca
      181 cgcgccgaga tatccgaaac aatccctgat cagccgccca ctaaaattgc gatccaaggg
      241 cagtacagtc tcccgtactg gtatgatccg caaggcaagc ctcgcacctt tgcgtgccga
      301 accataaccg tatcgccgtt ccaaatgacg ctcgccgttg cggtccccgg caaaatcgga
      361 aatcgcatta actcctgctt tcccgatctg ggtaacatgg gtggcagcat caccgatacc
      421 ttcgcaggcg gtttcgtgtt tgagccggac ctgactgaca tcgagcgcga gagaatggcc
      481 agcaagctcg tttggttaga aaagaaacaa cgcgatcccg ccgttcgcga tgctaggagg
      541 cacgagcgtt tcattcccgc aaatccgcat tcgaccctga cgttggcaga tggcagcact
      601 cgaagctgct ttgtgatcga tatgtcggtc accggtgtag cggtgtcggc cgcggtccag
      661 ccgccaatcg gagcagcgct cgccatcgga gcctgcgtcg gccgggtcgt aagattattg
      721 ccggagggtt ttgctatcca gttcgccgag acccaacgcc ggcgtgatct caatcggctc
      781 ctcctccgag tcgacccggc gcccgccagc agaactggat cctgaggtcc tccggccctg
      841 acacccgccc gaacggcgcc tgaggtacga gccgccgggc aaagctggct acagaacgcc
      901 gacggccaag aactcctccg ccctgttcat cttgctgttt ggtcatctgg cgtgcgccct
      961 gtcgaacgta gccgggcact cgcctgctcg tcgtcagggc aaaacgttca aggcccaaat
     1021 ctgatccgct ccggatgcac gccagagaag cgaccaccct cccccgacga ggtcgcgcgg
     1081 atagcttcag gttgcgcgcc tctaattttg ataaacgagc cttgccctga tggtcccgtc
     1141 aagcgagcca atgtcctcga gaactccctg actttcgtct ccgtgcacat ccacgtcgag
     1201 cacgacgtag cctatctcgc tgtcggtctc caggtactgt gctacgatat tgatgtcgcg
     1261 ctccaggaat acctcgttca gccgcctaag cattcctggc acattgcgat gaacatgggc
     1321 gaatcgggtc ccctgctgac gcttttgcaa atgaacttgc gggaaattca cggcgcccac
     1381 cgtcgagccg gtggtgatgt aatcgaccat cttccgggcc acttccttac ctattcggtc
     1441 ttgcgcctct tccgtcgaac cgccgatgtg gggtgtcagg atcacgttcg ttaagccttg
     1501 cacggggctt tgaaagtgat ccgaattgga agagggttcg actggaaaaa cgtcgagcgc
     1561 cgctcctgcc aggtgaccat cccgcagcgc ctccgccaga gcgtcgacgt cgaccaccgt
     1621 gccgcggcta ttgtttatga ggcacgcgcc gcgtttcatc aggcgaagtt cggactgacc
     1681 aatcatgccg gacgtggcca atgtctcggg cacatgcagg ctgacgacat cactttgcgc
     1741 cagcagatcc tgaaggctct cgaccggctc ggtgttacca tggcgaagtt tgtcggtttg
     1801 atcgaagtag atcactttca tgcccatcgc ttctgccagc gtcgacagct gcgatccaat
     1861 attgccatag ccgacaattc caagcgtgcg gccgcgcacc tcgtggctgt ttgtcgcgga
     1921 tttgtcccat tttccctggt gagcggccac acagcgagga aagatccgtc gaa
//
LOCUS       DASWQN010000322         4794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2406435, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000322 DASWQN010000000
VERSION     DASWQN010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2406435"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VGC82_05315"
     CDS             <1..696
                     /locus_tag="VGC82_05315"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY0422725.1"
                     /translation="QADKAVADAQATLDALRREGSDQPLQTLTAPFDGIVATIPIAQG
                     DRVQPGATLMTITRLDGLVVTVGIEPGARARVHPGEAVHLTPLSDGPPLEGHIVRVDG
                     MLNPKTRLLDADVSVPPGSVISGTAYRADITVGEIKGWIVPHDAVLTDSKGAYLFQVA
                     GASATHVDVKVAGTAGSDDVVQGTLDPERPIVVQGNYQLSDNTPVRISSRPRDSPPPD
                     GPPPDSKPKGGGE"
     gene            693..3824
                     /locus_tag="VGC82_05320"
     CDS             693..3824
                     /locus_tag="VGC82_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0422726.1"
                     /translation="MNFAAFVERHVRSILLVAFALAAAGLVAARSLPVGLFPQVSFPR
                     VVVDLDAGSRPADQTALLVTRPVEEAIRSVPGVLDVRSASSRGSAQISIDFGWGRDMI
                     SSTLLVDAAISQILPSLPSGTGYAVRRMDPTVFPIVSYALLSDTVSPVALQDLAQYQI
                     VPLLSAIPGLARVNVQGGDTAEIEVLADPHRLEAYGLGMTDLSDAVSKGNVLQAVGQL
                     QDNHKLYLVMADHSIGTIDKIGDVVVRSDAAGIVRVRDIASVQNGIVPQWIRVVEDGK
                     PAVLFNVYEQPDGNAVQIAGAVRAKLDQFKLPSGVRLVNWYDQSELVTQSASSVRDAV
                     LIGLVLAALVLLLFLRSWRVTLIAVLVVPATLAASILVLSLLGMSFNIMTLGGIAAAV
                     GLLIDDVIVMVEHIARRAGAPASGGGVVGSAAVLPAGREFLRPLTGSSLATLIVFVPL
                     SFLSGVTGAFSQALSITMAAALAISYLMTAFVVPVLALQMVDFDRWHDPGAAGTGWFA
                     RGHGRLLDILFARPWLFGIILVPLLAVGWFAYSAVPTGFMPAVDEGGFILDYYTPPGT
                     SLTETSREVGQIDDMLGAIPEVATFSRRLGTGLGADLGESYHGDYFVRLKPDHARSTT
                     AVMAAVLADVQAKVPGVQIELAQLMEDLIGDLTAVPQPIEIKLYASDTSALIPQAEKI
                     AAAIGKIDGVVEVKSGVKLAGDALDLTIDPIRAGIEGVTPDDVSRALSSALSGSVATQ
                     LPQATKTVGVRVRLSDALTLRQTALADLPIRATDGHIFPLHRVATMVPVTGQPEISRE
                     NLQPMIAVTARIEGRGIGAAVGDVQQVLGQPNMLGAGVRYEMGGLYQQQQIAFAGLTR
                     VFATALVAEFILLLFLYERLWLPVIIIACSLLSTTAVFTALWITGVDLNITALMGMTM
                     IIGIGTEMAIFYVSEYAELAPAMDPRRALREASRNRLRPITMTTLAAILTLMPLALAI
                     GQGSAIQQPLAISIIAGLLLQFPLVLLVMPVLIGFTIKPPSGAKIQPIEGGGSGNDEC
                     QYGDAQQSSRRYA"
     gene            3772..4551
                     /locus_tag="VGC82_05325"
     CDS             3772..4551
                     /locus_tag="VGC82_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422727.1"
                     /translation="MTNANTAMLNKVPVVTPSFWIIKIMATTVGETGADYLAVHVGFG
                     TAVTGTIMATLLLAAFLLQLRANTYVPWIYWLTVVLVSVVGTQITDALTDGLEVSLYL
                     STAVFAVLLATIFALWYAVERTLSIHTIVTRRRELFYWAAVLCTFALGTAAGDLATEA
                     LNLGFTLGVGVFGLLIAIIATAYYLGVNAVLTFWLAYILTRPLGASLGDLLSQSQTNG
                     GVGLGTIVTSVIFLVVISILVLRLTILQSWPPISLPSVDDS"
BASE COUNT          786 a         1508 c         1506 g          994 t
ORIGIN      
        1 caggccgaca aggctgtcgc tgacgcacag gcgacactcg atgccttgcg ccgggaaggc
       61 tcggaccagc cgttgcagac gttgacggca ccgttcgacg gaatcgttgc gacaataccg
      121 atcgcacagg gggaccgcgt gcagccgggt gcgacgttga tgacgatcac ccgcctcgac
      181 ggcctggtcg tgaccgtcgg catcgagcct ggcgcgcggg cgcgcgttca tcccggggag
      241 gcggtccatc tgacgcccct gtccgacggg ccgccgctcg agggacacat cgtgcgcgtt
      301 gacggcatgc tcaatccgaa aacgcggctg ctcgatgccg acgtctcggt gccgcccggc
      361 agcgttattt ccggtacggc ctaccgagca gacataacgg ttggcgagat caagggctgg
      421 atcgtgccgc acgatgcggt gctgacggac tccaaaggcg cctatttgtt ccaggtcgcc
      481 ggagcctccg caacgcatgt ggacgtgaag gtcgccggca ctgccggaag cgatgacgtc
      541 gttcagggga cactcgatcc tgagcggccg atcgtcgttc aaggcaatta ccagttgtcc
      601 gacaatacgc ccgtgcgcat cagcagtagg ccccgggata gcccgccccc agacggcccg
      661 cccccggaca gcaagccaaa aggcggcggg gaatgaactt tgcggcgttc gtcgagcggc
      721 acgtccggtc gatcctgctt gtggcatttg ccttggcggc ggccggcctg gtcgccgcgc
      781 gctccttgcc ggttgggctc ttcccgcagg tctcgttccc gcgggtggtg gtcgatctcg
      841 atgccggcag ccgcccggcc gatcagaccg ccttgctggt cacgcgaccc gttgaggaag
      901 cgatccgttc ggtgccgggc gtgctggatg tccgctccgc cagttcgcgc ggttcggcac
      961 agatttcgat cgatttcggc tggggccgcg acatgatcag cagcacgctg ctggttgacg
     1021 cggcgatatc gcaaatcctc cccagcctgc cgtccggcac cggatatgcc gtacgccgca
     1081 tggatccgac ggtcttcccg atcgtttcct atgccttgtt gtccgacacc gtttccccgg
     1141 tcgcgctgca ggatctggca cagtaccaga tcgtgccgct gctgtccgcg atccccgggc
     1201 ttgcacgggt caacgtccaa ggtggcgaca ccgccgagat cgaggtgttg gccgacccgc
     1261 accggctcga agcctatggc ctcggcatga cggatctgtc ggatgcggtg tcgaagggca
     1321 acgtgctgca ggccgttggc cagttgcagg acaaccacaa gctttatctg gtgatggcgg
     1381 atcacagcat cggcacgatc gacaagatcg gcgacgtggt ggtgcgatcg gacgctgccg
     1441 ggatcgttcg cgtgcgcgat atcgccagcg tgcagaacgg catcgtgccg caatggatcc
     1501 gggtcgtcga agacggcaag ccagcggtgc tgttcaatgt ctatgagcag ccggacggca
     1561 acgcggtgca gatcgccggt gccgtgcggg ccaagctcga ccagttcaag ctgccgtcgg
     1621 gcgtgcgtct ggtgaactgg tacgaccaga gtgagctcgt tacgcagtcg gccagcagcg
     1681 tgcgcgacgc ggtgctgatc ggtcttgttc tcgccgccct ggtgctgctg ctgttcctgc
     1741 gcagctggcg cgtgacattg atcgccgtgc tggtcgttcc ggccaccctc gccgcctcga
     1801 tcctcgtact gagcctgctc ggcatgagct tcaacatcat gacgcttggc gggattgccg
     1861 ccgccgtcgg cctgcttatc gacgatgtca tcgtgatggt cgagcatatc gcacgccggg
     1921 ccggcgcgcc ggcctcaggc ggcggcgtcg ttggcagcgc cgccgtgctc cccgccggcc
     1981 gggagttcct gcgcccgctg accgggtcca gcctggcgac gctgatcgtc ttcgtgccgt
     2041 tgagcttcct tagcggcgtc accggcgcgt tttcccaggc tttgtccatc accatggccg
     2101 cggccctggc gatttcctac ttgatgacgg ccttcgtggt gccggtgctg gcactccaga
     2161 tggtggactt cgaccgatgg cacgatcccg gcgcggccgg taccggctgg ttcgcccgcg
     2221 gacacggccg actgctggat atcctgttcg cccgcccctg gcttttcggt atcatcctgg
     2281 tgccgttgct cgccgtcggc tggttcgcct acagcgcggt gcccaccggt ttcatgccgg
     2341 cggtcgatga gggcggcttc atcctggact actacactcc acccggcacg tccttaaccg
     2401 agacaagccg cgaggtcggg cagatcgacg acatgctcgg cgccatacct gaggtagcaa
     2461 cgttctcccg ccgtctcggc accggtttgg gcgctgacct gggcgaaagc tatcacggcg
     2521 actactttgt ccggctgaag ccagatcatg cccggtccac gaccgcggtg atggcagcgg
     2581 tgctggcgga cgtgcaggcg aaagtgcccg gcgtgcaaat cgaactggcg caattgatgg
     2641 aagacctgat cggcgatctg accgccgtcc cgcagccgat cgagatcaag ctttacgcat
     2701 ccgacacttc agcgctgatc ccgcaggccg agaagatcgc cgcggccatt ggcaagatcg
     2761 atggagtcgt ggaggtgaaa agcggtgtca agctggccgg cgatgcactg gacctgacga
     2821 tagacccgat ccgcgccggc atcgagggtg taacccctga cgacgtctcc cgcgcactct
     2881 cctcagcctt gagcggttcg gttgcgacgc aattgccgca agccacgaag accgtgggcg
     2941 tgcgggttcg cctttccgat gcgctgacgc tgcggcagac agcgttggcc gacctgccga
     3001 tacgggccac ggacgggcac atatttccgc tgcaccgggt tgccaccatg gtgccggtga
     3061 ccggtcagcc ggaaatttcc cgggaaaatc ttcagccgat gatcgcggtg acggcacgga
     3121 tcgaaggccg cggcataggg gctgccgttg gtgacgtgca gcaggtcctg ggtcagccga
     3181 acatgctcgg cgccggcgtc cgatacgaga tgggtggttt gtaccagcag cagcagattg
     3241 ccttcgccgg tctcacgcgc gtcttcgcca cggccctcgt cgccgaattt attcttctgt
     3301 tgtttctcta cgagaggctg tggctgcccg tcatcatcat tgcctgttcg ctgctgtcca
     3361 cgacggctgt attcacggca ctttggatca ccggggtgga cctgaacatc acggcgctga
     3421 tgggcatgac gatgatcatc ggcatcggca ccgagatggc gatcttctac gtctcggaat
     3481 atgccgaact ggctcctgcg atggatccgc ggcgcgccct gcgggaagca agccgtaacc
     3541 ggctgcgccc catcaccatg accacgctgg ccgcgatcct gaccttgatg ccgcttgcac
     3601 tcgcgatcgg gcagggttcc gcgattcagc agccgctggc gatttcgatc atcgctgggc
     3661 tgctcctgca gtttccgctc gtgctcttgg tcatgcccgt cctgatcggc tttacgataa
     3721 agcctccgtc cggggccaaa attcaaccaa ttgaaggtgg ggggtcaggt aatgacgaat
     3781 gccaatacgg cgatgctcaa caaagttccc gtcgttacgc ctagcttctg gatcatcaag
     3841 atcatggcaa ccaccgtcgg cgaaaccggc gcggactatt tggctgtgca tgtgggtttt
     3901 ggcacagcgg tcacaggtac cattatggcg acgctgctgc tggcagcgtt tctgctccaa
     3961 ttacgagcaa atacctatgt cccgtggatt tactggctaa cggtggttct agtcagtgtc
     4021 gtcggaacgc agatcaccga cgctcttacc gatggattgg aagttagcct ctacctgagc
     4081 acggcggtgt ttgctgttct gctggctacg atattcgcgc tttggtatgc cgtggaacga
     4141 accctctcga tccacacaat cgttacgcgc cgtcgggagc tgttctattg ggcagcggtt
     4201 ttgtgcacct tcgcgctcgg caccgccgcc ggcgacctgg cgacggaagc gctcaatctt
     4261 ggcttcaccc tcggggtggg cgtgttcggc ttattaattg ccatcatcgc caccgcctac
     4321 tatctgggtg tcaatgccgt tctcacgttc tggctcgcgt acatcttaac ccggccattg
     4381 ggcgcctcgc ttggcgattt gctgtcccag tcgcagacca atggcggtgt cggcctgggc
     4441 acgatcgtga cgagcgtgat ctttctggtc gtcatttcga tattggtgct gcgcctcacc
     4501 atcttgcaaa gctggccgcc tatttcattg ccctcggttg acgacagttg agatggattc
     4561 gggaaacctc cgaattgcct gctgctcggc agcggcaata ggcgcctgtc cctccataag
     4621 ttcttatccc agagacttaa caaggagacg atcaatgaac cgttttgttt gtgcagcgct
     4681 cataattcca gcgtttggaa tgacgtctat aatcgccggc acagcccttc ctgtggcttc
     4741 gggcatcgct ttagccgcgg ctgcatccaa gatcggcgat ctgtcatcgt accg
//
LOCUS       DASWQN010000323         9001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1482875, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000323 DASWQN010000000
VERSION     DASWQN010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482875"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VGC82_05330"
     CDS             <1..656
                     /locus_tag="VGC82_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY0422728.1"
                     /translation="KLLFVLTFGKKLSVLSIIDPCLVAARHATRPLVHHVLRAVRHAG
                     SGIGRQGTHLVRHAGRAVGAHARRAVSVACNAAPGALAVGMLALPLPVIGPEQVPLGG
                     AVPLPKAAEMARPMMGFVPGFGPFQPATPDAGSIIVTFLPPEDVPAVNPGTLPGAGDS
                     PPPLQNISPSIPGAPPHSNFPISSDPGQPVPEPSSVLVLASALSGLSLLRWRTRRSS"
     gene            complement(726..3857)
                     /locus_tag="VGC82_05335"
     CDS             complement(726..3857)
                     /locus_tag="VGC82_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEY0422729.1"
                     /translation="MISSVFIERPRLAIVIAVLLTLAGALSLMRIPVSQFPDIVPPQV
                     TVSTSFTGASAAVVEATVAQPLEAAVVGVDKMIYMKSNSANDGSYSLSVSFELGTNPD
                     IDTVNVNNRVQQAIARLPVEVQRYGLTVRKRSAAILEFLQFYSEGGKQDPLFISNYVT
                     INVLDRLTRVPGVGDATLFGRLDYSMRIWFDMDRLVALNLAPADIVAVIQSQNVQAAV
                     GRIGARPTSDATQFQLSVQTQGRLTTAEQFGNIVIRANPDGSVLRIHDVARTELGAAN
                     SDTLSRLNGNPAVSIGIYLAPGANAVQTSARVSAALANLAPRFPESLKSRVFYDSSAF
                     VSSTIDEVEKTLLIAFVLVVAVVYVFLGSVRATIIPMVAIPVSLIGTFAGLLAFGFSA
                     NTISLLAMVLGIGVVVDDAIVVVENVERVMAEEPALSPKEATKKAMNQITGPVIAISL
                     VLLSVFVPVAFIPGLSGTLFRQFAVTISIAMLLSATIALTLSPALCALFLRHSRRPRG
                     PVGWMLTGIDKVRDGYSSIVNRLLRLAVLSVVAIVAAGIGIVLMGKITPTGFLPEEDQ
                     GALFTVVQLPDGASVERTRAVVEQVENLIRPMPQVEAVLSIVGFSLLDGGNQPNAAFL
                     VTRLKPFQDREGAKNGVAAVLGQIFGAAQQIRSAIVFPFNLPPIIGLSTSGGFEYQLE
                     NLEGRPPEDMASVMQGLVGVANRDPRLNRVFSTFTASNPSIWLDIDREKAQALGLSIS
                     DVFNALQTTLGGFYVNDFNLYGRTWQVNIQGDAIDRSDPTAIYKIYVRNRSGEMVPLR
                     SIASVRVQVGPQVISRYINYRSTTINGGPKPGVSSGDALVAMDQLSRTTLPPGYTFEW
                     TGTAYQEREASGQTGYILALSVLFAYLFLVALYESWMIPIPVLLSVTVGVLGAFFGLW
                     ISGLPLDLYAQIGMVVLIALAAKNGILIVEFAKEQREAGLSIRDAAASGARMRIRAVL
                     MTSIAFIFGLIPLVWAAGAAMLSRRAVGTAVFAGMIVASSIGVFLIPMLYVVFQTVRE
                     KAKQRFRRASAHV"
     gene            complement(3859..5025)
                     /locus_tag="VGC82_05340"
     CDS             complement(3859..5025)
                     /locus_tag="VGC82_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0422730.1"
                     /translation="MRFVGFILLGLILTLTSPARAQPPSGPPSVGVIRAQQTAITETS
                     EFVGRVQAIERVSLTARVTAFIDQRLFAEGSEVKQGDLLYRLERGPFEAAVQQQEAAV
                     ADISARLANASIQLSRAQSLMSGPAGQRSNVDDAIANQRSLAAQVLSAQAQLRVAQIN
                     LGYAEIRAPVDGKISRTSITMGNVVSPSSGPLASIVSQDPMYVLFPIAARTLAELRKR
                     YEDKGGTNAMVVKLRLPDGSLYSQNGKIDYVEPTVSATTDTILLRARIANPPRGATVA
                     GQPVERPLVDGAFVNVLVEGIQPVMALGIPRKAVLSDQQGDYVYVVGADNKVEQRRIQ
                     LGQSTPTTATVAGGLKEGEMVIADGIQRVRPGIEVNPGPASPPPPSAASGEGKS"
     gene            complement(5125..5862)
                     /locus_tag="VGC82_05345"
     CDS             complement(5125..5862)
                     /locus_tag="VGC82_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0422731.1"
                     /translation="MTLLSVSHLTKAFGGNEVVRGISFDIASGEIVALIGPNGAGKTT
                     CFNMLNGQLRPDGGEILFGGSSITSWTPDQIWRRGVGRGFQIPATFGSMTVRDNLRLA
                     LISRHQAVWRFFRPAANLLTEEADALLERVGLAHLAAKGCSTLAYGDLKRLELALAIS
                     NRPSLLLVDEPTAGMALPDRHGVMDLLLELARESDCAVLFTEHDTDTVFRVADRILVM
                     DKGELIADGDASAIRTNSRVRIAYLGA"
     gene            complement(5859..7664)
                     /locus_tag="VGC82_05350"
     CDS             complement(5859..7664)
                     /locus_tag="VGC82_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0422732.1"
                     /translation="MNFVAIQFLTGLSGSVSLFLTSAGLTVIFGVTRVVNFAHGSLFM
                     LGAYVGWSLLTRLPHDPFWFIAGVIGATLIVAALGAGLETALLRRLYHAPELFQLLAT
                     FGVLLVIQDATVMIWGPNDLAIPRPPWLRGYVSILGSRFPLYDLILIGVGPIVLAGLV
                     WLLAHTRFGMLIRACTENRDLAAALGIDQRMLSTTIFALGAGLAGLGGALILPDTSAN
                     TQMDLAVIVEAFVVVVVGGMGSVTGAFLASLLIGELQAFGIVVIPKATLVMMFVIMAV
                     VLGVRPAGLLGSIPLLTGRQASRSVFPPPSRDLRLLGLGMIALAATAPFWLPPYWLSV
                     LTEVLIAALFACSLHLMMGPGGMISFGHAAWFGLGAYAAALISKTLSAPMPVALLVAP
                     VVAGATAALFGWFVVRLSGVYLAMLTLAFAQIVWAIATQWTWLTGGDDGILGVWPTGP
                     VPFFWWVLALTVMAIWLLQRAIRAPFGLALMGGRDSELRALAIGLRPGRLRMVAFALS
                     GAAAGLSGGLFAFKTGSVFPAFVAVGRSVDGLIMVLLGGIDTLIGPIVGAVAYSGLYD
                     LLLRFVPLWRMALGLSIIALVLLFPSGLAAGRQPR"
     gene            complement(7761..8978)
                     /locus_tag="VGC82_05355"
                     /pseudo
     CDS             complement(7761..8978)
                     /locus_tag="VGC82_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1779 a         3009 c         2718 g         1495 t
ORIGIN      
        1 ttaagctact gttcgtgctt accttcggta agaagctgtc cgtgttaagc atcatcgatc
       61 cctgcctcgt tgccgctcgt catgccaccc ggccattggt tcaccatgtg ctgcgtgctg
      121 tgcgccatgc aggttccggg atcggacgcc aaggaacgca tttggttcgg catgccggtc
      181 gcgccgtggg tgcgcatgcg cgacgcgcgg tgtcggtggc ctgtaacgcc gcgcccggcg
      241 ctctggcagt cggcatgctt gcgctgccgc tgcccgtcat aggcccggag caggtgccgt
      301 tgggcggcgc tgttcccctt ccgaaagcgg ctgaaatggc gcgccccatg atgggattcg
      361 tgccagggtt tggtccattt cagccggcaa cgcccgatgc cggatcgatc atcgtgacct
      421 tcctgccgcc cgaggacgtg ccggcggtca atcctggcac tttgccaggc gcgggcgatt
      481 cgcctccgcc actccaaaat atctcgccgt caattcctgg cgcaccgccc cattccaatt
      541 ttccgatttc ctcggaccca ggtcagccgg tcccggagcc ttcctcggtc cttgtgctcg
      601 cctcggcgct cagcggcctg agtttgctgc ggtggaggac gcgccggtcg tcgtaatcga
      661 ccgatcgttg cccgcttccc agctcggcct accgaaacgt cggcctgatc agagcatgac
      721 ctgccctaaa catgcgccga tgctcggcgg aaccgctgct ttgccttctc ccggaccgtc
      781 tggaacacga cgtagagcat cgggatcagg aacaccccga tcgagctcgc cacgatcatg
      841 ccggcaaata ccgcagtgcc gacggctcgc ctgctcagca tcgccgcacc ggctgcccaa
      901 accaatggga tcagcccgaa gataaacgcg atggaggtca tcagcaccgc ccggatccgc
      961 atccgagcgc ccgacgctgc cgcatcccgg atcgacaggc ctgcctcacg ctgctccttg
     1021 gcaaattcga cgatcagaat gccgttcttc gccgccaacg cgatcagcac gaccatgccg
     1081 atctgcgcat aaaggtccag cggcagtccc gagatccaca agccgaaaaa cgccccaagt
     1141 acacccacgg tgaccgacag cagcaccggg atcgggatca tccagctctc gtaaagcgcc
     1201 accaggaaca ggtatgcgaa cagcactgac aacgcgagga tgtaccccgt ctgccccgag
     1261 gcctcacgct cctgataggc cgttcccgtc cattcgaacg tgtagccagg cggtagcgtc
     1321 gtgcgggaaa gctggtccat cgccaccaag gcatcgccgg acgatacacc cggcttcggg
     1381 ccgccgttga tcgtcgtgct ccggtagttg atgtagcggc tgatcacctg cggaccgacc
     1441 tgcacgcgga cactggcgat ggaacgaagc ggcaccatct cgccgctcct gttgcggaca
     1501 tagatcttgt agattgccgt agggtcgctc cggtcgatcg catcgccctg tatgttgacc
     1561 tgccaggtgc gcccgtaaag gttgaagtcg ttgacgtaaa agccgcccaa cgtggtctgc
     1621 agcgcattga acacgtcgga aatcgacagt ccgagcgcct gggccttttc gcggtcgatg
     1681 tccagccaga tcgacgggtt ggaggcggtg aacgtcgaaa atacgcggtt cagccggggg
     1741 tcccggttgg ctaccccgac aaggccctgc atcacgctgg ccatatcctc gggcggccga
     1801 ccttccaggt tctccaactg atattcgaag ccgccgctgg tggacaggcc gatgatcggc
     1861 ggcaggttga acggaaatac aatggccgaa cggatctgct gcgcagcccc gaatatctgg
     1921 cccagcaccg ccgcaacgcc atttttcgct ccctcgcggt cctggaacgg cttcaaccgc
     1981 gtgaccagaa acgccgcgtt tggttggttt ccgccgtcca gcagggagaa cccgacgatc
     2041 gacagcaccg cctccacctg cggcattggc cgaatgaggt tttccacctg ctcgaccacc
     2101 gctcgtgtgc gctcgaccga cgctccgtcg ggcaactgca cgacagtgaa cagcgcgccc
     2161 tggtcttcct cgggcaggaa gccggtcggg gtgatcttgc ccatcagcac gatgccgatg
     2221 cccgccgcga cgatggcgac caccgaaagc acagccagcc gcagcagccg gttgacaatg
     2281 ctggagtagc cgtcccgcac cttgtcgatc ccggtcagca tccatcccac tggcccgcgc
     2341 ggacggcggc tgtggcgcag gaacagtgcg cacaatgccg gtgacagcgt cagcgcgatc
     2401 gtcgccgaca gtaacatcgc gatgctgatc gtcaccgcga actgacggaa caatgttccc
     2461 gacaatcccg gaatgaaagc caccgggaca aacaccgaca gcagcaccag cgagattgcg
     2521 atgaccggcc cggtgatctg gttcattgcc ttcttcgtcg cttccttggg cgacaatgcc
     2581 ggctcctcgg ccatcacccg ctcgacattc tccaccacca cgatcgcgtc atcgacgacc
     2641 accccgatcc ccagcaccat ggcgagcaac gagatcgtgt tggcggaaaa gccgaaggcc
     2701 agcaggccgg caaaggtgcc gatcaggctg accggaatcg cgaccatcgg aatgattgtc
     2761 gcgcgcacgc tgcccaggaa gacgtacacc accgcgacga ccaggacgaa ggcgatcaac
     2821 agcgtcttct ccacctcatc gatggtggag gaaacaaaag cgctgctgtc gtaaaagacc
     2881 cgcgacttga ggctttccgg gaagcgcggc gccagatttg ccagcgccgc actgacccgg
     2941 gcggatgtct gcacggcgtt cgcgccgggc gcgagataga tgccgatcga aaccgccgga
     3001 ttgccgttca gccggctgag cgtatcgctg ttcgccgccc ccagttcagt tcgggcaacg
     3061 tcatgaatac gcaataccga tccatctgga ttggcccgga tgacgatatt gccgaattgt
     3121 tccgcggtgg tcagccgccc ctgggtctgc acgctaagct ggaattgcgt ggcgtcgctg
     3181 gtcggccggg caccgatgcg tcccacggcg gcctggacgt tctgcgactg gatgaccgcg
     3241 acgatgtctg ccggtgccag gttcaacgcc accaggcggt ccatgtcgaa ccatatccgc
     3301 atcgagtaat ccaggcggcc gaacaatgtg gcgtcgccga cgcccggcac ccgggtcagc
     3361 cggtcgagca cgttgatggt cacgtagttg ctgatgaaca gcggatcctg cttcccgcct
     3421 tcgctgtaaa actgcaggaa ttccaggatc gccgcggacc gcttgcggac cgtcaggccg
     3481 taacgctgca cctcgaccgg cagtcgcgcg atggcctgct gcacgcggtt gttgacgttg
     3541 accgtgtcga tatccgggtt ggtccccagc tcgaagctga cgctcagcga atagctgccg
     3601 tcgttcgcgc tgttggactt catgtagatc atcttgtcca cgccgacgac cgcggcttcc
     3661 agcggctggg cgacggtcgc ctccaccacc gcggccgagg cgccggtaaa gctggtggaa
     3721 acggtgacct gaggcgggac gatgtcggga aactgcgaaa ccggaatgcg catcagcgac
     3781 agcgcaccgg ccagggtcaa cagaacggcg ataacgatcg caagcctggg tcgctcgatg
     3841 aaaaccgagg agatcatgct acgattttcc ctcgcccgat gcagcggagg gcggtggcgg
     3901 gctggccggt ccgggattga cctcgatacc cgggcggacg cgctgaatgc cgtcggcaat
     3961 caccatttcg ccctctttca ggccacccgc gaccgtcgcg gtcgtgggcg tcgattgccc
     4021 cagttgaatt cgccgctgct ccaccttgtt gtcggcgccc acgacataga cgtagtcgcc
     4081 ctgctggtcc gacagcaccg ccttgcgggg aatgccaagc gccatgacgg gttgaatgcc
     4141 ttcgaccagg acgttgacga aagccccgtc aaccaacggc cgctcgaccg gttgccccgc
     4201 gaccgtcgca ccgcgcggcg gattagcaat tcgggcgcga agcaggatag tatccgtcgt
     4261 ggcggaaacc gtaggttcga cgtaatcgat cttgccgttc tgactgtata aggagccgtc
     4321 cggcaggcgc agcttgacga ccatcgcgtt ggtgccgccc ttgtcctcat agcgcttgcg
     4381 caactccgcc agggtccgcg cggcgatcgg gaacagcaca tacatcgggt cctggctgac
     4441 gatggacgcc agcggtcccg aactgggcga cacgacattg cccatcgtga tgctggtccg
     4501 gctgatcttg ccgtcaaccg gagctcggat ttccgcatac cccaggttga tctgcgccac
     4561 gcgcaattgc gcctgcgccg aaagaacctg agccgccagc gaccgctgat tggcgatggc
     4621 gtcatcgacg ttcgaacgct gcccagccgg cccgctcatc agcgattgtg ctcgggacag
     4681 ctggatgctg gcattggcca gacgcgcgct gatatcggcc acggcggctt cctgttgctg
     4741 cacggcagcc tcgaacgggc cgcgctcaag ccggtacagc aggtcgccct gcttgacctc
     4801 gctgccttcg gcgaacaggc gctggtcgat gaaagcggtg acccgagcgg tcagggacac
     4861 ccgctcgatc gcctggacgc gtccgacgaa ttccgacgtt tccgtgatgg ctgtctgctg
     4921 cgcgcgaatc acgccaacgg atggcgggcc gctaggtggc tgggcccgcg ccggggatgt
     4981 aagcgtaaga attaaaccca gtagtatgaa gccaacgaaa cgcatcaggc gagatccccg
     5041 gcgcctgctg tggcgcagca tgaaaatgag atggatgccc ggactcggca acagggcgtt
     5101 ctttatattc ggctaacaaa atgttcatgc accgagatag gcgattctga cccggctgtt
     5161 cgtccggatc gcggaggcgt caccatccgc gatcaactca cccttatcca tgaccagaat
     5221 ccggtcggca acccggaaaa cagtgtcggt atcgtgttcg gtaaaaagga cggcacaatc
     5281 gctttctcgt gccagttcca gcagcagatc catcacacca tgccgatccg gcagcgccat
     5341 gccggcagtc ggctcatcca caagcagcag gctcggccgg ttcgatatcg ccaacgccag
     5401 ttccagccgc ttcaggtcgc catacgcgag cgtggagcaa cccttcgccg cgagatgggc
     5461 cagtccgacc cgttccaaaa gagcgtcagc ctcttcagtc aacaggtttg ctgccggccg
     5521 gaagaagcgc cagaccgcct gatggcggga gatcagcgcc aaacgcaaat tgtctcgcac
     5581 ggtcatggag ccgaacgtcg ccgggatctg gaagcctcgg cccacgccgc gacgccagat
     5641 ctggtccggt gtccatgacg tgatgctgga gccgccgaac aggatttcgc cgccgtcggg
     5701 ccgtaattgc ccgttgagca tgttgaagca ggtggtcttc ccggcgccat tcgggccgat
     5761 caacgccacg atctcgccgg aggcgatgtc aaacgaaatc ccacgaacga cttcgttgcc
     5821 accgaaagcc ttcgtcaaat gactgaccga aagcagcgtc atcgcggctg gcggccggcc
     5881 gcgaggccac tgggaaacag cagaaccagg gcgatgatcg acaaaccgag cgccatccgc
     5941 cacagcggca cgaaccgcag cagcagatcg taaagcccgg aataggcgac ggcgccgacg
     6001 atgggcccga tcaaggtgtc gattccgccc agcagcacca tgataagccc atcgaccgat
     6061 cggccgaccg cgacaaaggc gggaaagacg ctgccggtct tgaaggcgaa caaaccaccc
     6121 gagagtcccg cggccgcgcc cgacagggcg aacgctacca ttcgcagccg gcccggccgc
     6181 aatccgatcg ccagtgcccg aagttcgctg tcacgacccc ccatcagcgc caatccgaac
     6241 ggcgctcgaa tggcacgctg cagcagccag atcgccatca cggtcagtgc cagcacccac
     6301 cagaaaaacg gcaccggacc agtgggccaa acgccgagga ttccgtcgtc gccacctgtc
     6361 agccacgtcc actgcgtggc aatggcccag acgatctggg caaaggccag cgtgagcatc
     6421 gccagataga cgcctgacaa acggacgacg aaccagccga acaacgccgc tgtcgctccg
     6481 gcgaccactg gcgcaaccag cagcgcgacc ggcattggtg ccgacaaggt tttcgagatc
     6541 agcgccgccg cataggcccc cagcccgaac caggcggcgt ggccaaaact gatcatgcca
     6601 cccggcccca tcatcagatg caggctgcag gcgaacaggg cggcgatcaa cacctcggtc
     6661 agcaccgaca gccagtaggg cggaagccag aatggcgccg tcgcggccag cgcgatcatg
     6721 cccaggccca gcaggcgaag gtcacgtgac ggcggaggaa aaacgctcct gctcgcctgc
     6781 cgcccggtca gcagtggaat gctcccaagc aagcccgccg gccgcacgcc gaggacgacc
     6841 gccatgatga caaacatcat tacgagcgtt gccttgggaa tgaccacgat gccgaaagcc
     6901 tgcaactcgc cgatcagcag gctggcgagg aacgcgccgg tcacgctccc catgccaccg
     6961 accacgacga cgacgaacgc ctcaacgatc accgccaggt ccatctgcgt gttggccgat
     7021 gtgtccggca ggatcagcgc gccgcccagt ccggccaacc cggcgcccaa agcgaagatc
     7081 gtcgtggaca gcatcctctg gtcgatgccg agtgcggcgg ccaggtcgcg gttttcggtg
     7141 caggcgcgga tcagcatacc gaaccgcgtg tgcgccaaca gccaaaccaa gcccgccaac
     7201 acgattggcc cgaccccgat caggatcagg tcatacagcg ggaaccggga cccgaggatc
     7261 gagacgtagc cgcgcaacca aggcggcctg gggatggcaa ggtcgttggg accccagatc
     7321 atgaccgtcg cgtcctggat caccaacaaa acgccgaagg tcgccagcaa ctgaaacaac
     7381 tccggcgcgt ggtagaggcg ccgcagcagc gcggtttcca gccccgctcc aagcgctgcg
     7441 acgatcaagg tcgcgccgat cacccctgcg atgaaccaga acgggtcgtg cggcaggcgt
     7501 gtcagaaggc tccaaccgac gtaggcgccc agcatgaaca ggctgccatg ggcaaaattg
     7561 acaacccggg tgacgccgaa gatgacggtc agccccgcgg aggtcaaaaa cagcgaaacc
     7621 gacccggaca gacccgtcag gaactggatg gcgacgaagt tcacgaatca tgtcatgacc
     7681 gggcttggcc cggtcaccca ccactccgca gcgccggtcg cacagcactt gtgtcggacc
     7741 aaaaccgtga cgccctcaag tcaaccagca ttcggccgca gcgtcttcac cacctggtcg
     7801 gtcggcaaat aggccgcccc atcggcgtag cgccaccccg tcatcatgcc gttgccgttc
     7861 ttccggacag tcttgcccac aaaagtgcca agcgttccct gatggtcgag gccgcggtag
     7921 ctggctgggc cgaacggcgt gtcgaacgtc accccgctga agccttccac caatttgtcc
     7981 acgttcaacg atgctgcccg cgtgatgccc gcggcgatcg accggatcag cgtgtagccc
     8041 accaccgaac ccatccccgg cgttttgttg aagcgggcct gataagctgc gatgaactcc
     8101 tggttcgcct tggtctcgag ctgcccttgc ggatagcccg tgacaatcca cccttccgga
     8161 gtttcgtcac ccagcggctc caggtactcc ggttcgccgg tcaggacgga gacgaccgac
     8221 cggttctcga acagcccgcg cgtattgccc tggcggacga agttggtcag gtcgggacca
     8281 aaggtgacat tcaggatggc gtcaggcttc gcctcggcca gcgcggcggc ggtcgccccg
     8341 gcatcaatct ttcccagtgc cggccactgt tcgccgacaa atgcaacgtc gggacgtttg
     8401 gcggtcagca acgccttgaa ccacttcacc gtcgaaagcc cgtactcgta gttcggagcg
     8461 acggtgaccc aggtcttggc cggcagcttc gccgcttcct cgaccagcat agcggactac
     8521 atatacgtgg acggacgcag ccggaagcag tagcggttgc ctttctccca gaccagcgca
     8581 tcggacagcg gctcgccggc gacatacagg cacttccgct gcgaggcgta atccgacaac
     8641 gcaaggccca cgttcgacag gtagcctcca gccaggatgt ccaccttctg ctcgtccagg
     8701 agttcgcccg cgagccggat ggtatcctgt ggcttgcccg cgtcgtcgcg gctgatgacc
     8761 tcaagcttcc ggcccagtac gccgccggcc gcgttgatct cctcggccga aagctgccag
     8821 ccgttgcggt atggcaaggt gaacgaggga atggcggagt aggagttgat ctcgccaatt
     8881 cggatcggtg cctgctgtcc ccgcaacagg gttggcgcgg acagaacgcc ggccgtccct
     8941 agcccggcgg ttcccagcag cgttcggcgc ttgatcataa ggaagagtcc cggcctgttg
     9001 c
//
LOCUS       DASWQN010000324         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_32264, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000324 DASWQN010000000
VERSION     DASWQN010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32264"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1863
                     /locus_tag="VGC82_05360"
     CDS             262..1863
                     /locus_tag="VGC82_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0422733.1"
                     /translation="MTALWWQTGVIYQIYPRSFQDADNDGIGDLKGIERRLEYLISLG
                     VEAIWISPIYPSPMVDFGYDVTDYCDVDPRFGTLADFDDLLMQAHRRGIKVLLDFVPN
                     HTSDRHLWFAESRSSQENPKRNWYIWRDPAADGGPPNNWISDFGGSAWEWDQITRQYY
                     YHAFLKEQPDLNWRHPAVQAAMYDVMRFWFDRGVDGFRIDVLWHMLKAADFPNNPPNP
                     AYRPVMGDMYRLLQHHSTDQPEVHLIAAEMRDIADGYGASGQGERVLIGEVYLPVDRL
                     MQYYGGERPGVHLPFNFQLIDTPWDARALAAAITTYEASLPPDGWPNWVLGNHDRPRV
                     AARRGQAQARVAAVLLLTLRGTPTLYYGDELGLIDVVIEPSQVRDPRELREPGLALGR
                     DPVRTPMPWDDSENAGFTRGTPWLPLHADWPTRNMMRMAEEPQSLLTLYRRLLALRRG
                     CPALSIGDFALLNVEDDTLVYARRHGEERLIVALNFGTRQHRLALPDWARGGRVLLSS
                     AEDAAPAEVGTLLLGPNEAVVLEAR"
     gene            1889..>2531
                     /locus_tag="VGC82_05365"
     CDS             1889..>2531
                     /locus_tag="VGC82_05365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0422734.1"
                     /translation="MRIAMPAPISWRTPPRHYGPWEQVTSLLTEALVARGVDVTLFAT
                     KDSLTAARLDAVCPAPYSEDPTIDAKVWEMLHVAHVFEQAGQFDLIHNQADFVPLAFS
                     RLVKTPVVTTIHGFSSARILPAYKEYEARVHYVAISDADRHPDLRYAATIHHGIPLQD
                     FPFDPLGGEDLLFFGRIHPDKGAAEAIAAAHRTGRKLTIAGIVQDRNYYDQHVA"
BASE COUNT          469 a          782 c          789 g          491 t
ORIGIN      
        1 agcacgcgtt caggtgcatt gattgcaatc accttgaagg tgcgatggtc gagggccaaa
       61 ggagcaccgc gatcgcggct tgattggcat ctgatcggag atagagacgg tcttcgggcg
      121 gccgttttgt ttcttggcag ccgactagga gttgcgctta ttaaagccaa tgcggttgga
      181 cgcgaagggt atggtggaac agtgccgccc tcatgcggtc ggtaaacggc aatctgctca
      241 catgccaaag gtgctgtttg catgaccgcg ctctggtggc agacgggtgt gatctatcag
      301 atctatccgc ggtcgtttca ggacgctgac aatgacggca tcggcgacct caaggggatc
      361 gagcggcggc tcgaatatct tatcagtctt ggggtcgaag cgatctggat ctcccccatc
      421 tacccgtcgc ccatggttga tttcggctat gacgtcaccg actattgcga cgtcgatcca
      481 cgcttcggta cgctggcaga cttcgacgac ctgcttatgc aagcccatcg gcgggggatt
      541 aaggttctgc tcgacttcgt gcccaaccac acatccgacc ggcacctttg gtttgccgag
      601 agccgctctt cgcaagaaaa tccgaaaaga aactggtaca tctggcgcga tcctgcggct
      661 gacggcggac caccgaacaa ctggatcagc gacttcggcg gctcagcctg ggaatgggac
      721 cagataacca gacaatatta ctaccacgcc tttctgaagg agcagccaga cctcaactgg
      781 cgtcatccag ctgtgcaagc ggcgatgtac gatgtgatgc gtttttggtt cgaccgtggc
      841 gtagatggct tccgcatcga cgtgctgtgg cacatgctaa aggcggcaga cttcccgaac
      901 aatccgccca acccggccta tcggccggtg atgggcgaca tgtatcgcct gcttcaacac
      961 cattcgacag accagcccga ggtgcatctg atcgctgccg aaatgcgcga catcgccgac
     1021 ggctacggcg ccagcggaca aggcgagcgg gttctgattg gtgaggtcta cctgccagtc
     1081 gatcggctaa tgcagtatta cggcggtgaa cgcccagggg tgcacctgcc cttcaatttc
     1141 cagctcatcg atacgccgtg ggatgcgcgc gccctggcag cagcgatcac gacctatgag
     1201 gcctcgttac cgccggatgg ttggcccaac tgggtgctcg gcaaccatga ccggccgcgc
     1261 gttgcggcca ggcgcgggca ggcgcaggcc cgcgtcgcgg cggtgttgtt gctcacgcta
     1321 cgcggcacgc ccacgctcta ttacggcgac gaactggggt tgatcgatgt cgtgatcgaa
     1381 ccgtcgcagg tgcgcgatcc gcgcgagttg cgtgagcccg gtctggcgtt gggccgcgac
     1441 cccgtgcgga cgccgatgcc gtgggatgac agcgaaaatg ccggcttcac ccggggcacg
     1501 ccgtggctgc cgctccatgc cgactggccg acgcgcaaca tgatgcgcat ggcggaagaa
     1561 cctcagtccc tcctgacgct ttatcgccgg ttgcttgcgc tcaggcgagg ttgcccggca
     1621 ctgtcgatcg gggacttcgc tctgttgaat gtggaagacg acacactggt ttacgcccgc
     1681 cggcacggcg aggagcggtt gatcgtggcg ctcaatttcg gcacgagaca gcaccggctt
     1741 gcgttgccgg actgggcgcg cggcggccgt gtgctgctgt cctctgccga ggatgccgca
     1801 ccggcggaag tgggcacgct cttgttaggg ccgaatgaag ctgtcgtgct cgaagcccga
     1861 tagcaccagg caaagaaggg acgttatctt gcgcatcgcc atgccggcgc cgatctcctg
     1921 gcgcacaccg ccgcgccact acggcccctg ggagcaggtg acgagtctcc tgaccgaggc
     1981 gctggtggcg cgcggcgtcg atgtcacttt gttcgccacc aaggacagcc tgacggcggc
     2041 caggctcgat gcggtctgtc cggcgcccta ttccgaagac cccacgatcg acgccaaggt
     2101 gtgggagatg ctgcacgtcg cccatgtctt cgaacaggcc ggtcagttcg acctgattca
     2161 caatcaggcc gatttcgtac cgctcgcgtt ctcgcggctg gtcaagacac ccgtggtgac
     2221 gacgatccac ggtttttcgt ccgcgcgcat tctgcccgcc tacaaggaat acgaagcgcg
     2281 cgtccactat gtcgccatca gcgacgccga ccgccatccg gacttgcgct atgccgcaac
     2341 gatccaccac ggcatcccgc tgcaggattt ccccttcgac ccgcttggcg gcgaagatct
     2401 gctgttcttc ggccgcattc atccggacaa gggcgccgcc gaagcgatcg cggcggcaca
     2461 tcgcacggga cggaaattga ccatcgccgg catcgtccag gaccgcaatt attatgacca
     2521 gcacgtggcg c
//
LOCUS       DASWQN010000325         5502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_164037, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000325 DASWQN010000000
VERSION     DASWQN010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164037"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VGC82_05370"
     CDS             complement(<1..340)
                     /locus_tag="VGC82_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0422735.1"
                     /translation="MAEEIAFHRRRFLGTASMIFATARFGSVGSAEAQPGKTRPADLP
                     TVRPGTNTSFASMKQITAGVLNVGYAEAGPADGSVVILLHGWPYDIHSFTDVAPLLAS
                     AGYRVIVPYLR"
     gene            complement(493..1887)
                     /locus_tag="VGC82_05375"
     CDS             complement(493..1887)
                     /locus_tag="VGC82_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0422736.1"
                     /translation="MSDPHWLSASEISTAYAAKTLSPVELLTALLARIEALDPKLHAF
                     IRLDPDAAMQAARQAEAEIAAGRKRGKLHGVPVGIKDIIDVAGLPTTGHSKILLDNIA
                     KSDAAVVQKLRQAGAVIMGKLSTHEFAIGGPSFDLPFPPARNPWNPDHHPGGSSSGSG
                     AGVAAGLFPLALGTDTGGSVRNPASACGIVGLKPTYGLVSRRGVFPLSFTLDHVGPLT
                     RSVADSALLLDAIAGYDPADPGSAATPAHGFGRLLDRGVRDLRIGFVRHFHERDMPAD
                     PEVGAALEDAARVLQAEGAQVHTVTLPALTEFAGINRVILCSEAWSIHAPWLRERPGD
                     YGKLARRRLLPGAFMTAGDYVGAQRRRAELIAAVEDRLRDYDVLLCASSMDPASRLDD
                     AEETARTYPRQARTPFNVTGHPALAMMAGLSRAGLPVSVQFAGRYFDDATVLRVAAAY
                     ERATGWHEKKPPIG"
     gene            complement(1884..2063)
                     /locus_tag="VGC82_05380"
     CDS             complement(1884..2063)
                     /locus_tag="VGC82_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422737.1"
                     /translation="MDPTIIALARAAGLDKALNDFPDDVSAAAEQALNNAGAVLVPTD
                     PAAEPWPPMRAGAGL"
     gene            2413..3594
                     /locus_tag="VGC82_05385"
     CDS             2413..3594
                     /locus_tag="VGC82_05385"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0422738.1"
                     /translation="MTTLPLVGLKILEIGHYIAAPFCTRILADLGAEVIKLEPPGGDP
                     FRGWGAAKDGNSVWFSIHGRNKLSVELDLKKDRDTVLKLVGRADVLVENLRAGQLERL
                     RIGPDELHAVNPRLVIVRISGYGQDGPYRDKPAFGAIGEAVGGLRHLTAHPNGTTDLP
                     PPRCGISISDDLAGLYAAIGLLSAIWQRDAMGTGKGRIVDVNLVDSVFSLMEGMLPEY
                     ALDGRIRQPAGAALPTASPTNTYPCADGRWLCIAGNSDLIFSRLMTAIGRPEMAADPT
                     FKTNGLRCEHRDALDAAISAWTRTMAAKDAEAVLEAAEVPCSRLFDIADCAADPHFRA
                     RHAVQEIQDPLIGRTLHPGPSIRMDNERPEDVVRWTGPAVGAHTDHVLHTLLGLPREP
                     T"
     gene            3591..4154
                     /locus_tag="VGC82_05390"
     CDS             3591..4154
                     /locus_tag="VGC82_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEY0422739.1"
                     /translation="MIGCIVHPSFRRNPGLVPLFCLALAACGNSGEPRAGDYIGGTTA
                     VECAPFARALTGVSLTGAAADWWSQADGRYARIRQPVVGSVLVFRRSARLPYGHVAVV
                     SRVLSGRQIMVTQANWVHHRVTEDQPVLDVSETGDWSLVRVWWPPSGQIGLADYPIHG
                     FIRPDHPTEHDRLVAETPAAIRLAAGR"
     gene            complement(4187..4654)
                     /locus_tag="VGC82_05395"
     CDS             complement(4187..4654)
                     /locus_tag="VGC82_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY0422740.1"
                     /translation="MSTLISAVPSSEEPSFATTLFVRDNCLCLHVQRAARALARRYDD
                     VLRPVGLTSGQFSLLMSLNRPEPPNIAQVATLLAMDRTTLTANIKPLERLGLIEVGRV
                     ATDKRQRTLMLTPAGRSALAAAVPLWQRTHDAIENLLRESGPDRLRTDLRTLS"
     gene            4701..>5502
                     /locus_tag="VGC82_05400"
     CDS             4701..>5502
                     /locus_tag="VGC82_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEY0422741.1"
                     /translation="MTTRVLVLLGTKKGAFILESGAARETWSLRGPFCETWPMNHVIA
                     DPATGTIYGAGGNEWFGPAVWKSNDLGQSWTHSSDGLSYEKGQDAVATVWSLAARGGR
                     LYAGVQPAGLFSSDDAGQSWTHIAGLQAHPTRPKWQPGGAGLILHSIVTHPDDPDQIW
                     IGISAAGVFHTADGGRTWEPRNSGTRCDFLPEGERYPEFGQCVHCIVMAPNQPGRLYQ
                     QNHCGMYRSDDGGRNWDSIEAGLPSSFGFPAVAHPRDPSTVFLLPLNGD"
BASE COUNT         1006 a         1779 c         1793 g          924 t
ORIGIN      
        1 cgcgcaggta agggacgatc acccggtagc ctgccgacgc cagcaacggg gcgacatcgg
       61 taaagctgtg aatgtcgtag ggccagccgt gtagaagaat gacgacggag ccatcggcgg
      121 gaccagcctc ggcatatccg acgttcagga cgccggcggt gatctgcttc atggaagcga
      181 acgacgtatt cgtccccggc ctgactgtcg gcagatccgc tggccttgtc ttgccgggtt
      241 gtgcttccgc agaaccgacc gaaccgaacc gggcggtggc gaaaatcatg gaagcggtgc
      301 ccaggaagcg gcggcgatga aaggcgattt cctcagccat tttctctctc cagctcaatg
      361 acaacaacgt tgtcgcttgt ggcgtgggat tacggcgcac atcgggcgca aaaaagcttc
      421 cccacacaca tcattgccgg gcggtcgtcc cttcgtgccg ggccgcaccg agtgcgccgt
      481 agaccgacgg ggctaaccga ttggcggctt cttctcgtgc cagccggtcg cccgctcata
      541 ggcggcggcg acacgcagca ccgtggcatc gtcgaaatac cgcccggcga actgcacgga
      601 caccggcagt ccggctcggg acaagcccgc catcatcgcc aacgccggat gcccggtcac
      661 attgaacgga gtcctggcct gacgcgggta ggtacgcgcg gtctcctcgg catcgtccag
      721 gcggcttgcc gggtccatcg agcttgcaca cagcagcacg tcgtagtcac gcaaccggtc
      781 ctcgaccgcg gcgatcaact cggcacgccg gcgctgggcg ccgacatagt cgccggcggt
      841 catgaacgca cccggcagca gccggcgccg agcgagcttg ccgtagtcgc ccggccgttc
      901 ccgtagccag ggggcatgga tggaccacgc ctcgctgcac agaatcacgc gattgatgcc
      961 tgcgaattcc gtcagcgccg gcagcgtgac ggtgtggacc tgggcgccct cggcttgcag
     1021 gacacgagcg gcatcctcca gcgcggcgcc gacctccgga tcggcgggca tgtcgcgctc
     1081 atggaaatgc cggacgaagc cgatgcgcag gtcgcggacg ccgcggtcca gcaatcggcc
     1141 gaaaccgtgc gccggcgtag cggcgctgcc agggtcggcc ggatcgtagc cggcgatggc
     1201 atccagcagc agcgcgctgt cggcaaccga acgcgtcagc gggccgacat ggtccagcgt
     1261 gaaggacagc gggaacaccc cacgccgcga caccaggccg taggtcggtt tcaacccgac
     1321 gatgccgcac gcgctggcgg ggttgcgcac cgaaccgccg gtgtcggtgc cgagggccag
     1381 cggaaacaat ccggccgcga ctcccgcgcc ggatcccgaa gaggacccgc ccggatgatg
     1441 atccgggttc cacggattgc gcgccggtgg aaacggcagg tcgaaactgg ggccgccaat
     1501 ggcgaattca tgggtggaca gtttgcccat gatcaccgcg ccggcctggc gcagtttctg
     1561 gacgaccgcc gcatcggact tcgcgatgtt atccagcagg atcttcgaat gccccgtggt
     1621 cggcagcccg gcgacgtcga tgatatcctt gatccccacc ggcacgccat gcagcttccc
     1681 acgcttgcgc cccgcggcga tctccgcttc ggcttgcctg gcagcctgca ttgccgcatc
     1741 ggggtccaac cggataaagg catgaagctt cggatcaagc gcctcgatcc gcgccagcag
     1801 agccgtgagc agttccaccg gcgacagcgt cttcgccgca taggccgttg agatctccga
     1861 ggcggacagc cagtgcggat cgctcatagc ccggcaccgg cgcgcatcgg cggccagggt
     1921 tcggcagccg gatcggtcgg caccagaacg gcgccggcat tgttcagggc ctgttcggcc
     1981 gccgcgctaa cgtcatcggg aaaatcattg agtgccttgt cgaggccggc cgctcgtgca
     2041 agggcaataa tggtcggatc catggtagcg ctccgcgacg atgtgcggga cgatcatgcg
     2101 acgtgaagcg ccgcccgaaa agagccggga ccgcttagcc cagccgctgg ctgcgcacac
     2161 tggagaaaag aatcggccca ggataaaagc accaccagga caaagcgcga caggttgaaa
     2221 aaaagcggca tggccgggga caggccatgc cgcgaaaggt ggccgcgcca tgcgcgactg
     2281 tcaggaagga aacaagacac tgccaaagcc gcgtcggatt tctatttaca tgaaccatgt
     2341 tgacgaagag ttaacaacta cgtgttcgga tcgaatattt ttgattacgt ttcgaaaaag
     2401 aggtgtcgaa cgatgacaac tctacctctg gttggactga aaatcctcga aatcggccac
     2461 tatatcgcgg cgccgttctg tacccgcatc ctcgcggacc tcggtgccga agtcataaag
     2521 ctggagccac cgggcggcga tccgtttcgc ggctggggcg cggcaaagga tggcaactcg
     2581 gtctggttca gcattcatgg ccgcaataaa ctctctgtcg aactggattt gaagaaggac
     2641 cgcgacaccg tcctgaagct cgtgggccgg gcggatgtgc tggtggagaa tctacgggca
     2701 ggccagttgg agcgtttgag gatcggtccg gacgaattgc atgcggtcaa tcctcgcttg
     2761 gtgatcgtcc ggatatcagg ctatggccag gatgggccgt atcgcgacaa gccggcattc
     2821 ggtgcgatcg gcgaggccgt cggcgggctg cgtcatttga ccgctcatcc gaacggcacg
     2881 accgatctgc cgccgccacg ctgtggcatt tcgatcagcg acgaccttgc cggtttgtat
     2941 gccgccatcg gattgctgtc ggcgatctgg cagcgcgatg cgatgggtac cggcaagggg
     3001 cgcatcgtcg acgtcaatct cgtggacagc gtgttcagcc tgatggaagg catgttgccg
     3061 gaatacgcgt tggacggacg gatccgccag ccagccggcg ccgcgcttcc caccgcctca
     3121 ccgaccaaca cctatccctg cgccgatgga cggtggctgt gcatcgccgg gaactcggat
     3181 ctgatcttct cgcgtttgat gacggcgatc ggccggccgg agatggcggc ggaccccacg
     3241 ttcaagacca acgggttgcg ctgcgaacac cgcgacgcgc tggatgcggc gatcagcgcg
     3301 tggacgcgga cgatggcggc gaaggacgcc gaagccgtcc tggaggcggc cgaagttccc
     3361 tgctcccgct tgttcgacat cgccgattgc gcggccgatc cgcatttccg cgcgcgccat
     3421 gcggtgcagg agatccagga cccgctgatc ggacgcactt tgcatccggg accgtctatc
     3481 cgcatggaca acgagcggcc cgaggacgtc gtccggtgga ccggccctgc ggtcggtgcg
     3541 cacaccgacc acgtgctcca cacgctgttg ggcctgccgc gcgaaccgac atgatcggtt
     3601 gcatcgtaca tccttcgttt cgtcgcaacc ccggtctggt tcccctgttc tgcctggcgc
     3661 tggccgcgtg cggcaactcc ggagagccgc gcgccggcga ctatatcgga ggcaccaccg
     3721 ccgtcgaatg cgccccattc gcgcgcgcct tgaccggagt ctcgctgacc ggagccgcgg
     3781 cggactggtg gtcacaggcc gatggccgct acgcgcgaat tcgccagccg gtcgtcggca
     3841 gcgtgttggt gttccgccgc tccgcccgcc tgccgtacgg ccatgtcgcc gtcgtgtcgc
     3901 gggtgctgtc cgggcgccaa atcatggtga cgcaggcaaa ttgggtgcat caccgggtca
     3961 ccgaagacca gccggtgctc gatgtttccg aaaccggcga ctggagcctg gtgcgcgtct
     4021 ggtggccgcc gtccgggcaa atcggcctgg cggattatcc tatccatggc tttatccggc
     4081 ccgaccatcc aaccgaacac gaccgcctcg tcgccgagac gcccgccgcg attcgactgg
     4141 ctgcggggcg ttgacagtgc tggggcttta acagtgccga ggcgtgttag gatagcgtcc
     4201 gcaggtcggt ccgaagccga tcgggaccgg attcccgtag tagattttca atcgcgtcat
     4261 gggtccgctg ccaaagcggc acggcggccg caagagctga tcgcccggcc ggcgtcaaca
     4321 tcaacgtccg ttgacgtttg tccgtcgcca ccctgccgac ctcgatcaac ccaagccgct
     4381 caagcggctt gatgttcgcc gtcagcgtcg tgcggtccat tgccagcaac gtcgcgacct
     4441 gggcgatgtt cggcggttcg gggcggttga gcgacatcag caacgaaaac tggccgctgg
     4501 tcagcccgac gggccgcaac acgtcgtcgt aacgccgcgc cagggcacgc gccgcccgct
     4561 gcacatgcag acacaggcag ttgtcgcgga cgaacaacgt ggtggcgaaa gatggttcct
     4621 ctgatgacgg cacggcgctg attagagttg acatcaacct atttgtccag gaactctgcc
     4681 gcgatcgagg gggaaaactt atgacaacac gcgttctcgt tctgctcgga accaagaagg
     4741 gcgccttcat cctggaaagc ggtgcggcac gtgagacctg gtcgctacgg ggcccattct
     4801 gcgagacctg gccgatgaac catgttatcg ccgatcccgc gaccgggacg atctatggcg
     4861 ccggcggcaa cgagtggttt ggccccgccg tctggaaatc caacgatctc ggccagagct
     4921 ggacgcattc aagcgatggt ctgtcttacg aaaagggcca ggacgcagtc gccacggtct
     4981 ggagcctcgc tgcgcgcggt ggccgactct acgccggcgt ccagcctgcc gggttgttca
     5041 gcagcgacga tgccggccag tcctggaccc acatcgccgg cctgcaagcg caccccacgc
     5101 ggccaaaatg gcagcccggc ggcgcggggt tgatcctgca ttcgatcgtc acccatccgg
     5161 acgacccgga tcagatctgg atcggcatat cggcagccgg cgtgttccac accgccgacg
     5221 gcggacgaac ctgggaaccg cggaacagcg gcacccgctg cgactttctg cccgaggggg
     5281 agcggtaccc cgagttcggc cagtgcgtgc actgcatcgt catggctcct aaccagccgg
     5341 gccggctgta tcagcagaac cactgcggca tgtatcgaag cgacgacggc ggccgcaatt
     5401 gggacagcat cgaagcgggt ctgccatcca gcttcggttt cccggcggtc gctcacccga
     5461 gggatccatc gaccgtgttc ctgttgccgc tcaacggcga ca
//
LOCUS       DASWQN010000326         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_427897, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000326 DASWQN010000000
VERSION     DASWQN010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427897"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..930
                     /locus_tag="VGC82_05405"
     CDS             196..930
                     /locus_tag="VGC82_05405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0422742.1"
                     /translation="MIDQQIDIATKDGKTTTFITHPERGGPFPVIIFYMDAPAIREEL
                     RDMARRLGTAGYYVMLPNMYYRASVMELGPINPDPESPERKKMFELMHSLTIPLVMED
                     TKALLAYAETQQAANTKIIGTVGYCMSGRYAVNAATHFPDRVKAAASIYGVQLATDQP
                     DSPHLAAGKTKAELYFGCAETDVYAPPEIVEKVREGMKGTKNEVELYPGTHHGFAFPK
                     RPIYDRDAAERHWERLLALYRRNLVA"
     gene            946..1776
                     /gene="lgt"
                     /locus_tag="VGC82_05410"
     CDS             946..1776
                     /gene="lgt"
                     /locus_tag="VGC82_05410"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY0422743.1"
                     /translation="MPFLTIAFPVFNPIALQLGPIAIRWYALAYICGIVLGWLYARSL
                     VKKERLWGGPAPITLLQLDDFILWVTIGIIVGGRTGYVLFYNPSFFFAHPAVFFQLGN
                     GGMSFHGGFLGCVAAVMLFARSNGISILSLGDITTAVGPIGLFLGRLANLINSELWGR
                     PADSSVPWAMVFPNGGPEPRHPSQLYEAGLEGILLFTILAIMIRMGALKRPGLILGSF
                     IAIYAFARIASEFFREPDAQLGFLWGGLTMGMLLSVPMIIVGIILIVQAWRRETPEHI
                     "
BASE COUNT          371 a          600 c          569 g          389 t
ORIGIN      
        1 gttacgcaag actttgtcag agaagacaat ggcgatgcca agcgcctgga tcaaggtccc
       61 ggatcaaggc cccggatcaa cgggcgcggt tcaaggccaa tgtgatggcc ctttgccttg
      121 tcacgccacg gtttccttgc aatcgtattc atgcgcccaa tcagaaacca gaatcataac
      181 aggcaagaaa cgcccatgat cgaccagcaa atcgacattg cgaccaagga cggcaagacc
      241 accaccttca tcacccatcc cgaacgcggc gggccgtttc cggtcatcat tttctacatg
      301 gacgcgccgg caatccgcga agaactgcgc gacatggcgc ggcggctcgg caccgcgggc
      361 tactacgtga tgctgcccaa catgtattac cgcgccagcg tcatggaact cgggccgatc
      421 aatcccgatc cggaatcgcc cgagcgcaag aagatgttcg agctgatgca ctcgctgacc
      481 attccgctgg tcatggaaga caccaaggcc ctgctcgctt atgccgaaac ccagcaggcc
      541 gcgaacacca agatcatcgg caccgtcggc tactgcatga gcgggcgcta tgcggtcaac
      601 gcggcgacgc attttcccga tcgcgtcaag gcggcggcat cgatctacgg cgtgcagctc
      661 gccaccgacc agcccgacag tccgcatctg gccgccggca agaccaaggc cgaactctat
      721 ttcggctgcg ccgagaccga cgtctatgcg ccgccggaaa tcgtcgagaa ggtgcgcgaa
      781 ggcatgaagg gcaccaagaa cgaggtcgaa ctctatcccg gcacccatca cggttttgca
      841 ttcccgaagc gcccgatcta cgaccgcgac gccgccgagc ggcattggga gcggctattg
      901 gcgctctatc gccgcaacct cgtcgcctag agccatcgat actcgatgcc gtttctcacc
      961 atcgcctttc cggtattcaa cccgatcgcg ctccagctcg ggccgatcgc gatccgctgg
     1021 tatgcgctgg cctatatctg cggcatcgtg cttggctggc tctacgcccg ttcgctggtg
     1081 aagaaggaga ggctgtgggg tgggccggcg ccgatcacgc tgctgcagct cgacgatttc
     1141 atcctctggg tgacgatcgg catcatcgtc ggcggccgca ccggctacgt gctgttctac
     1201 aatccatcct tcttcttcgc gcaccctgcc gtatttttcc aattggggaa cggcggcatg
     1261 tcgtttcacg gcggttttct cggctgcgtc gccgcggtga tgctgtttgc ccgcagcaac
     1321 gggatttcga tcctctcgct cggcgacatc accaccgcgg tcgggccgat cgggttgttc
     1381 ctcgggcggc tcgccaacct catcaacagc gagctgtggg gccggccggc ggattccagc
     1441 gtgccgtggg cgatggtatt tcccaatggc ggaccggagc cgcggcatcc aagtcagctc
     1501 tacgaagcgg ggctcgaggg catcctgctg ttcacgatct tggccatcat gatccggatg
     1561 ggcgctctga aacggccggg gctgatcctc ggcagtttca ttgcgatcta tgcctttgcg
     1621 cgtattgcga gtgaattctt cagagagccg gacgcccagc tcggattttt gtggggcggg
     1681 ctgacaatgg gtatgctgct gtcggtgccg atgattattg tcggcattat ccttatagtg
     1741 caggcatggc gccgcgagac gccggagcat atttagaagt ccatttaagg caggtcgtga
     1801 cagaattttc gccactgcag tcggagatca agaagctgat caaatcgtcg ggaccgatgc
     1861 cggtctggcg atacctggaa ttgtgcctgc tgcatccgga gcacggctac tacgtgtcgc
     1921 gcgatccgc
//
LOCUS       DASWQN010000327         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_691417, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000327 DASWQN010000000
VERSION     DASWQN010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691417"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2759)
                     /gene="dnaE"
                     /locus_tag="VGC82_05415"
     CDS             complement(<1..2759)
                     /gene="dnaE"
                     /locus_tag="VGC82_05415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY0422744.1"
                     /translation="MSAGFAHLRVHSAYSLSEGAIRPDKIAALAKDAGMPAAAITDTG
                     NLFGALEFSQYCAGKGIQPIIGCQIALARTDASRLPPDPLVLLAQDATGMANLQLLSS
                     FGFLDTDPTLKPQLPFQRIADNAAGLLLLTGGTTGPIGRLLAEGQKAEAERLLDAMRE
                     AFPGRTIQELHRHSLPVEAAIEPGLIGLADARGIPLVATNDCYFATRDMHEAHDALLC
                     IAEGRTLSEQDRRRVTPEHWFKPAADMRALFADLPEACDNTLAIARRCAAMAETRKPL
                     LPVCPKVRPGASEEETVRAMAMEGLERRMDTIAADTARREIYRVRLEYELSVIASMGF
                     SGYFLIVADFIQWAKAQGIPVGPGRGSGAGSVAAWALTITDLDPLRFNLLFERFLNPE
                     RVSMPDFDIDFCQEGRDRVIDYVRNEYGGDRVAQIITFGKLQARAAVRDVGRVLGLPF
                     GQVNRVAELIPNNPAKPVTLQQAVDGEPRLQTLRDEDEGIGRLLEIALRVEGLYRHAS
                     THAAGVVIGDRPLIELVPLYRDPKSDFLVTQYSMKHVEQAGLVKFDFLGLTTLTILRR
                     AEKFLAGLGITVDLDRLPLDDKKTFEMLQKGDAGGVFQLEGQGMRDTLRQMRPDRFED
                     LIAAVALYRPGPMANIPDYCRRKHGEKWEPPHPELAEILGETYGIMVYQEQVMQIAQK
                     MAGYSLGSADLLRRAMGKKIAVEMEAQRNVFTEGAIKRGIEPAKATEVFDLMAKFADY
                     GFNKSHAAAYALVAYQTAWLKANHPEVFIASCMSLALNNTERLAALKQEAERSSIKIL
                     PPDINRSSADFAVERLDDGRLAIRYALAAVKKVGFVAMEAMTAARGGTPFADVTDFAA
                     RVDPRQISRMQVENLVRAGAFDELAKNRARLFAGAETIIRRAQADQEEKESGQIGLFG
                     GITSKP"
BASE COUNT          473 a          869 c          934 g          535 t
ORIGIN      
        1 ggcttgctgg tgatcccccc gaagagcccg atctggccgc tctctttttc ctcctggtcg
       61 gcttgtgctc ggcgtatgat tgtttccgct ccggcgaaca ggcgcgcgcg gtttttcgca
      121 agctcgtcga acgcgccggc ccgcacaagg ttctcgacct gcatacggct gatctgccgt
      181 ggatcgacac gggcggcgaa gtcggtgacg tcggcgaatg gggttccgcc gcgtgccgcc
      241 gtcatggctt ccatggccac gaagccgacc ttctttaccg ccgccagcgc atagcggatc
      301 gctagccgcc cgtcgtcgag gcgctcgacc gcgaaatccg ccgaggaccg attgatatcg
      361 ggcggcagga tcttgatcga actgcgttcg gcttcctgct tcagcgccgc caggcgctca
      421 gtgttgttga gcgccaagct catgcaggag gcgatgaata cttccggatg gttggccttc
      481 agccaggcgg tttgataagc gaccagggca tatgcggcgg cgtgtgattt gttgaagccg
      541 taatcggcga acttcgccat caggtcgaag acctccgtcg ccttcgccgg ttcgatgccg
      601 cgtttgatcg cgccctcggt aaagacgttg cgttgtgcct ccatctcaac ggcgatcttc
      661 ttgcccatgg cacggcgcag caggtcggcg ctgcccaggg aataacccgc catcttctgg
      721 gcgatctgca tcacctgttc ctggtaaacc atgatgccgt aggtctcgcc taagatttcg
      781 gccagttcgg ggtgcggcgg ctcccatttt tcgccatgct tgcgccggca gtaatcgggg
      841 atgttggcca tcgggcctgg acgatacaga gcgacggcag cgatcagatc ctcgaaccgg
      901 tcggggcgca tttgccgcag cgtgtcgcgc atgccctggc cttccaactg gaacacccca
      961 ccggcgtcgc ccttctgcag catttcgaag gttttcttgt catccagcgg cagtcggtcg
     1021 agatcgaccg tgatgccaag tcccgcaagg aatttttccg cgcggcgaag gattgtaagg
     1081 gtcgtcagcc caagaaaatc gaatttgacc agtcccgcct gctcgacgtg cttcatcgag
     1141 tattgcgtca ccaggaagtc cgatttcgga tcgcgataca gcggcacgag ttcgatcagc
     1201 ggacggtcgc caatcacgac gccggcggcg tgtgtggacg catgacgata caggccttcg
     1261 acgcgcaggg cgatttccag caggcggccg atgccctcat cctcatcgcg cagggtctgc
     1321 aaccggggtt cgccgtccac cgcctgctgc aatgttaccg gcttggctgg attgttcggg
     1381 atcagttcgg cgaccctgtt gacctgcccg aacggcagtc ccagcacgcg gcccacatcg
     1441 cgtaccgcgg ccctggcctg aagtttaccg aaagtaatga tctgggcgac ccggtcgccg
     1501 ccatattcat tgcggacata gtcgatgacg cggtcgcggc cttcctggca gaagtcgata
     1561 tcgaagtcgg gcatcgacac gcgttcgggg ttgaggaagc gttcgaacag caggttgaag
     1621 cgcaacggat cgaggtcggt gatggtcagc gcccaggcgg caacggaacc ggcacccgac
     1681 ccgcgtccgg ggccgaccgg aataccctgc gccttggccc actggatgaa gtcggcgacg
     1741 atcaggaagt agccggagaa ccccatggag gcgatgacgg aaagctcgta ttccagccgc
     1801 acccgataga tctcccggcg tgcggtgtcg gcagcgatgg tgtccatccg gcgttccaag
     1861 ccctccattg ccatggcgcg gacggtttcc tcctccgaag cgccaggacg gaccttgggg
     1921 cagaccggca gcagcggctt tcgggtttcc gccatggcgg cgcagcgccg ggcgatcgcc
     1981 agcgtgttgt cgcaggcctc tggaaggtcg gcgaacaggg cccgcatgtc ggcagccggc
     2041 ttgaaccaat gttcaggcgt gacgcggcgg cgatcctgct cggacagcgt acggccctcg
     2101 gcgatgcaca gcagggcatc gtgcgcctcg tgcatgtcgc gggttgcgaa gtagcagtcg
     2161 ttggtggcga ccagcggaat gccccttgcg tcggcaaggc cgatcaggcc gggttcgatg
     2221 gcggcttcga cgggcaggct gtggcggtgc agctcctgga tggtgcggcc ggggaaggct
     2281 tcccgcatcg cgtccagcag gcgttcggct tcagctttct gaccctcggc tagcaggcgg
     2341 ccgatcgggc cggtggtgcc gccggtcagc agcaacaagc cggcggcatt atccgcgatg
     2401 cgctggaacg gcagttgggg tttcagcgta ggatcggtgt ccaggaagcc gaacgaggac
     2461 agaagttgca ggttggccat gccggtggcg tcctgcgcca gcagcaccag tggatcgggc
     2521 ggcaggcgac tggcatcggt gcgggcgaga gcgatctggc agccgatgat cggctggatt
     2581 cctttgccgg cgcagtactg ggagaattcc aacgcgccaa acaagttgcc tgtgtcggtg
     2641 attgccgccg ccggcatgcc cgcgtccttc gccaacgcag cgatcttgtc ggggcgaatc
     2701 gcaccttcgc tgagcgagta ggcggagtgg acgcgcagat gagcgaagcc tgcgctcatg
     2761 ccgggaccac cctgccctcg tgcagcgtga cccggcggtc catgcgggca g
//
LOCUS       DASWQN010000328         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1879924, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000328 DASWQN010000000
VERSION     DASWQN010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879924"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..2299)
                     /locus_tag="VGC82_05420"
     CDS             complement(323..2299)
                     /locus_tag="VGC82_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422745.1"
                     /translation="MLREPPAAPQAFVLDRRRLLAAATTGIAMGSAGGSQHAPAQPAK
                     PTGADGPRRLSITDSAIADGDSLRVADFGLVGIFDIDWLLEPRFTRLLDNFAASPGAF
                     ASVRFFGALNSGEREGTLPTRSGGVWLRREDAPDFTTTLNALDALVSRGIVPFVTLSF
                     FPRAISESPVMPPRDFSVWADLVQAFVNAIVARFGAAEVGRWRFEVWNEPNYQPFWHG
                     DFDQYLDLYRSTSEAVSRTGHSVHLGGPALVYTPDSDGPVLMERFLRFLAEEPAMQCG
                     FISYHRKGAWFLDQDAPWLGGLQAAAQEIADAALRLVPERAKGLELINNEADMKVGFD
                     RPFEPRMNQRFPAWLAASMIVHEALTQKYSAQGIKFVAASDNANQHLIRTPFDGRRSV
                     MTRASDAPDDLVKLPVYAFYELLPLLGAQRATAVTRPAEAFFPSTDLLYLPTVSDSHL
                     SVLFACYPDRTKPTDPGGPGWTLDYNLTNIPWPRVNAVWFRIDDTHTNPLAAAGGADA
                     AAGLNNRATWRGVRAAQELGVAAPIRSGLTLADGGVFRDRIDLPAFATALLWITPFSA
                     NPPAAPVWTTAEASNGNALLRWTCAPDAKIYSYEVFRIGPDGQPGARLSPMPLRSALW
                     VDTAPPPGAQTYAVRAVSASGVLSRLVTGPLVHI"
BASE COUNT          468 a          705 c          747 g          415 t
ORIGIN      
        1 gctcttgttc taccatgccg aagatatagg gtagtcatca agctctaaca ggccctagtt
       61 cgagcggttt gggaaacgct ggggcctgtc gcgtcatcgg tggagattat cgactgcagt
      121 cgcaatctgc gccaagagtc agagtaagaa acaaacagga tcgcataaca tcgggttagc
      181 tacagcggct tcacggtagc tagaaacgga gttctcctgt ggctacaaag aaagtcgtgg
      241 gtggcgtgtc aagcacggct atgacccatc gacgccaaac cgccgaatgg ctcacccctt
      301 ttcaagtcac cgtgaaagcg ctctagatat gaaccaacgg tccagtaacg aggcggctca
      361 agacgccgga tgcggatacg gctcggacag cgtaggtttg cgctcccggt ggcggcgctg
      421 tgtccaccca cagcgccgac cgcagcggca taggtgacag acgcgcgccg ggctgtccgt
      481 cagggccgat gcgaaagacc tcgtagctgt agattttggc atcgggcgca caggtccatc
      541 gcagcagcgc gttcccgttg ctggcttccg ctgttgtcca caccggtgcg gccggtggat
      601 ttgcgctgaa cggagtgatc cacagcagtg cggtcgcgaa ggcgggaagg tcgatcctgt
      661 ctcggaacac gccgccgtcc gcaagggtca ggcccgaccg gatcggtgcg gccacgccca
      721 actcctgggc cgcgcgcacc ccgcgccacg ttgccctgtt gttcaatcca gccgccgcat
      781 ccgctcctcc ggccgctgcg agaggatttg tgtgcgtgtc gtcgatgcgg aaccaaacgg
      841 cgttgactcg cggccacggg atgttcgtaa ggttgtagtc cagcgtccaa ccgggtccgc
      901 caggatcggt gggtttggtc cggtccggat agcaggcgaa cagcacgctt agatgactgt
      961 cgctgactgt cggtaagtat aacaagtcgg ttgaaggaaa gaacgcctcg gcaggtctcg
     1021 taacggccgt ggcgcgttgc gcaccaagca agggcagcaa ctcatagaag gcgtaaaccg
     1081 ggagtttcac aagatcatcg ggcgcatcgg atgcccgggt catgaccgac cggcgtccat
     1141 cgaaaggcgt tcggatgagg tgctggttgg cgttgtcgga tgccgcgacg aactttatcc
     1201 cctgcgccga gtacttttgg gtcagcgcct cgtgtacaat cattgaggca gccagccagg
     1261 ccgggaagcg ttggttcatc cgcggttcga acggcctgtc gaagcccacc ttcatatctg
     1321 cttcgttgtt gatcagttcc agtcccttcg ctcgctcagg gaccagacgc agcgcggcat
     1381 cggcgatctc ctgcgccgcg gcttgcagtc ctccaagcca tggggcgtcc tggtcgagga
     1441 accacgcgcc cttgcggtgg taggatatga aaccgcactg catcgccggc tcctctgcca
     1501 gaaagcggag gaaacgttcc atcaatacag gtccgtcgct gtcgggcgta tagacgaggg
     1561 cggggccgcc gagatggacc gaatgcccgg tacgggaaac cgcctcgctg gtggatcggt
     1621 ataggtccag gtactggtcg aaatcgccgt gccaaaacgg ctgatagttg ggctcgttcc
     1681 acacctcgaa ccgccaccgt cccacctccg cggcgccgaa ccgagcgacg attgcgttga
     1741 cgaaggcttg cacgagatcc gcccagaccg agaaatcccg cggcggcatg accggagatt
     1801 ccgaaatcgc ccgggggaaa aaagacaacg tcacgaaggg cacgatgccg cgcgaaacga
     1861 gagcgtccag cgcattcagc gtggtcgtga agtctggtgc gtcctcgcgg cgaagccaga
     1921 cgccgccgct tcgtgtcgga agcgtgccct cgcgctcgcc gctgttcagg gcaccaaaaa
     1981 accgcacgga cgcgaatgcg ccgggagagg ccgcgaaatt gtccagcaac cgggtgaagc
     2041 gcggttccag cagccaatcg atgtcgaaga tcccaaccag gccgaaatcc gcgacccgga
     2101 gagaatcgcc gtcggcgatt gccgaatcgg tgatgctgag gcgtcttggc ccgtcggcgc
     2161 cggttggttt ggctggttgg gccggcgcat gttgactacc gccagccgac cccatggcga
     2221 tcccggtggt cgccgctgcc agcaggcgcc gccggtccag tacaaaagcc tggggagcag
     2281 cgggcggttc acgcaacata ggttctgcct ttcaactgca agccacaaaa gcggc
//
LOCUS       DASWQN010000329         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_428283, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000329 DASWQN010000000
VERSION     DASWQN010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428283"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            444..1430
                     /locus_tag="VGC82_05425"
     CDS             444..1430
                     /locus_tag="VGC82_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEY0422746.1"
                     /translation="MLSVYVPSESSSTLKKVPLEDPAALPEAAVWIDLVKPTSVEDHA
                     VERLAGIAVPTREDMQEIEISSRLYIENGARYMTATLMCHSDTDMPRTTAVTFILTGH
                     RLVTVRYDAPKPFALVENKLARSCMPAISGEMVLMELVDAVIDRCADILERAGGDMDH
                     ISHQIFEPEGAARSGHAKRYSDILIAIGRKGDLTSKVRESLVSIGRVVTFVAAAVDGV
                     KWSKDMREQLKTMQRDVASLTDHASYLSNKITFVLDAMLGVVNLEQNNIIKLFSVMAV
                     VLMPPTLIASIYGMNFKVMPELEWVHGYPMALVMMLLAAVLPYVFFKWKKWL"
BASE COUNT          284 a          452 c          477 g          304 t
ORIGIN      
        1 gggtgttggt cgcctcatag gccgtgctct gcatgcagcc tgacagcatc aggccggcag
       61 cgaggatacc cagtgtgact ttcagcgacg acatgattac ttccaatcga aagtcttgcg
      121 ttcgcggcgg caaatctgcg ccccaaacgc gacgattcca ttaatcccat tatcgccaaa
      181 aaccggcact tattccagtg tttgctgagt tgttcggcgc tgcgggaggc tggctgtggc
      241 ttttatgccg caagttttgc ggtttccggt tgatttctgg gcaggagtgg cgctggtgcc
      301 gatatctgtc catcgttggc cacctcgccg ccaccccatc gccataaaga tggaccggcg
      361 gttaatacgc cagtcatgaa gcgcttgcca gaatcgcgtt aagtgccgct gggtgcggtc
      421 tgtgccccgt tccggagttg cccatgctgt cggtgtacgt tccttccgag tcctcatcca
      481 ccctgaaaaa ggtccccttg gaggacccgg cggccctgcc ggaagccgcg gtctggatcg
      541 acctggtgaa gccgacctcg gtggaggacc acgccgtgga gcggctggcc gggatcgcgg
      601 ttccgacccg ggaggacatg caggaaatcg agatttccag ccgtctctat atcgagaacg
      661 gcgcccgcta catgaccgcg acgctgatgt gccattccga caccgatatg ccgaggacga
      721 cggccgtcac cttcattctg accggccacc gcctggtgac ggtgcggtac gacgcaccga
      781 agccgtttgc gctggtggaa aacaagctgg cgcggtcctg catgccggcc atctcgggcg
      841 aaatggtgct gatggaactc gtcgacgcgg tgatcgaccg ctgcgccgac attctggagc
      901 gcgccggcgg ggacatggac catatcagcc accagatctt cgagccggag ggcgcggcgc
      961 gcagcggcca cgccaagcgc tattccgaca tcctgatcgc gatcggccgc aagggggatc
     1021 tcacctcgaa agtccgcgag agcctggtct cgatcgggcg ggtggtgacc ttcgtcgccg
     1081 ccgcggtcga cggcgtcaaa tggtcgaagg acatgcgcga gcagctgaaa accatgcagc
     1141 gcgacgtggc ctcgctgacc gaccacgcct cctacctgtc gaacaagatc accttcgtgc
     1201 tcgacgccat gctcggcgtg gtcaacctcg agcagaacaa catcatcaag ctgttttcgg
     1261 tgatggcggt ggtcctgatg ccgccgaccc tgattgcctc gatctacggg atgaacttca
     1321 aggtgatgcc ggagctggaa tgggtgcacg gctacccgat ggcgctggtc atgatgctgc
     1381 tggccgcggt gctgccgtat gtgtttttca aatggaagaa gtggttgtag acgctaaaac
     1441 gtcttcacgt catccccgcg aaaggcaggg cgtgttggca aaattcggac gcatttgcgc
     1501 cgcgagaatg cggaggg
//
LOCUS       DASWQN010000330         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_164619, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000330 DASWQN010000000
VERSION     DASWQN010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164619"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VGC82_05430"
     CDS             <1..699
                     /locus_tag="VGC82_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEY0422747.1"
                     /translation="AADPLDATLHGLLELIERDAVALWLRGGVRPRVIPPAPGAGLLG
                     QLRGAAPKRRTWLLDITSDIGVPVVAAVACDDTGFGLCRGSACRPTLAAAADAALMEL
                     AQMEVAYRISATKQAMRGAEALNNTDREHILRYTAIDVGTTAALHPLAPPAPSCDLLA
                     HDKIAILVGVRKRLEAAGVEVYALNLTRPKLGIAVVRTIAPGLEVGLTSPPGPRLRSA
                     AEEAGIDLTRPILL"
     gene            745..2880
                     /locus_tag="VGC82_05435"
     CDS             745..2880
                     /locus_tag="VGC82_05435"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY0422748.1"
                     /translation="MNQAPDPKRMIPAPAPAPATATAEPKLRVSVIGGAHFSYGGREI
                     ALRNRKARAIFGYLALTPTGEDSREKLAGLFWSEFSEQNARATLRQAIHEFREALQEA
                     GCSAMFGTRTTVGLRPGSFSVDLDEILAAVAVREAPDVLLQQERLAETLLAGFDDLDP
                     SFHVWLMARRQALHDRLIRGLEEGYRDLTLDPRRRRLLAEAVLLLDPTHEEACRIVMR
                     CAAEAGETSTSIRAYDALYRLLDEDYDMEPSAATQELIAEVKQGKFDGPSDSRQDFDR
                     PSYVAEMKQALIAPRRAAPARDPPPIASKPALFVDAFAMSGIGPDSIHLVEGFRIELI
                     ACLSRFREWYVAGTDVNGEDDHEGVRVSSRYGVTTTAYQAGTTISVVMVLQERPSGMA
                     IWGERFELRLDKWLEAQQRIVRRIAATLNVQVSFERLARLSHMSDVSLESYDVWLRSQ
                     WITRNFSAEEWNRAAEMITMSIEKAPTFSSLYSGLAQMNNAAHIMQPGTFRDPKKIAR
                     TLALAEKAVALDPLDSRAELCLGWALAMSERYAAAEIHMELACSLNSNDSWTMISTAM
                     FYAFCGNAERAANLSAEAMGMTLSPIPRHWVYEATIRLLRGDNEGAIEAADRAQETLL
                     TLPALRAAALANLGRTREARLEAERFYDGARANWVNDEAPTNQTIARWLLQIFPISKR
                     EVWERLRDSLREAGIHVEGLTFAAQALTN"
     gene            complement(2956..3546)
                     /locus_tag="VGC82_05440"
     CDS             complement(2956..3546)
                     /locus_tag="VGC82_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422749.1"
                     /translation="MGTPTPLYDVKNLDQWGRLIKSWATHLDYVTDPTAPQPPRNYWV
                     NKTWGAATPPAATTTLDTDNEGKPKPWCLPPGGPVLVPDADGRSVVLPFATAMTTKEF
                     MTRVAAANVDVTVMPKQYTHVVIVQGNVETMVLRLPPKDTLQGSEDDLLNGTPYQIRP
                     FYGDLYSGPFHQPAGRIGILELHANRIGEYTLNNCM"
BASE COUNT          666 a         1125 c         1240 g          749 t
ORIGIN      
        1 gcagccgatc cgctcgacgc cactctgcac ggcttgctgg agctgatcga acgcgatgcc
       61 gttgctttgt ggctgcgcgg cggcgtgcgc ccgcgtgtga ttccgcctgc tcccggcgcc
      121 ggtctgctgg gacagttgcg cggcgcggcg ccgaagcggc gcacgtggct gctggacatc
      181 accagcgaca tcggcgttcc ggtggtcgcc gcggttgcct gcgacgatac cgggttcggg
      241 ttgtgccgtg ggtccgcgtg ccgcccgacc ctggcggccg cggcggatgc agccctgatg
      301 gagctggcac aaatggaagt ggcataccga atttcggcca cgaaacaagc catgcgtgga
      361 gcggaggcac tgaacaacac ggatcgggag catattcttc gttacaccgc gatcgatgtc
      421 gggacgacgg cggcactgca ccccctggcg ccccctgcac catcgtgcga tttattggca
      481 catgacaaga tcgcgattct ggttggtgtg cgaaagcggc tcgaagccgc gggggtggag
      541 gtttacgcgt tgaacctcac cagaccgaaa ctgggaattg cggttgtgcg gacgatcgcg
      601 ccgggactgg aggtggggct gacgtcgccg ccgggtccac gtctgcggtc cgccgccgag
      661 gaagccggga ttgatcttac ccggccaatc ctgttgtgat gccaatcctg ttgttatcat
      721 tgaaatcatg tcgttgctga cgaaatgaat caggcgcccg accctaagcg gatgattccc
      781 gccccggccc ccgctccggc cacggcgacc gcggagccga agctgcgtgt gtcggtgatc
      841 ggcggcgcgc attttagcta tggtgggcgg gagatcgcgc tgcgcaaccg caaggcgcgc
      901 gccatcttcg gctatctggc gctgaccccg accggggagg actcgcggga aaagctggcc
      961 ggcctgttct ggagcgagtt tagcgagcag aacgctcggg ctaccctgcg gcaggcgatc
     1021 cacgagttcc gcgaggcgct gcaggaggcc ggttgttccg cgatgttcgg cacacgcacg
     1081 actgtaggtt tgcgcccggg atccttttcg gtcgatctcg acgagatcct ggccgcggtg
     1141 gcggtgcgcg aggcgccgga tgtcctgctg caacaggagc gtctggcaga aacgctgctg
     1201 gcgggattcg atgacctcga cccgtcgttt cacgtctggc tcatggcacg gcggcaggcg
     1261 ctgcatgacc ggctgatacg cggcctggag gaaggctacc gcgacctgac gttggacccg
     1321 cggcggcggc ggcttctggc cgaagcggtg ctgctgctcg atcccacgca tgaggaggcc
     1381 tgtcgtatcg tcatgcgttg tgccgccgag gccggcgaaa caagtacctc gatccgggca
     1441 tacgatgcgt tgtaccggct gctggacgaa gattacgaca tggaaccgtc ggccgcgacg
     1501 caggaactga tcgccgaggt caaacaaggc aagtttgatg gcccgagcga ttcgcggcaa
     1561 gattttgacc ggccctccta cgtggccgag atgaagcagg cgttgatcgc gccccggcgc
     1621 gcggccccgg cgcgcgatcc gccgccgatc gcctcgaagc ccgcgttgtt cgttgacgcc
     1681 ttcgcgatga gcggcattgg tcccgacagc atccatctcg tggaagggtt tcgtatcgag
     1741 ttgatcgcct gcttgtcccg gttccgggaa tggtatgtcg ccggcaccga cgtcaacggc
     1801 gaagacgatc atgagggtgt gcgtgtttcg tcgcgctatg gcgtcaccac caccgcctat
     1861 caggccggaa ccacgatcag cgtggtcatg gtattgcagg aacggccgtc cgggatggcg
     1921 atctggggcg agcgcttcga gttgcggctg gataaatggc tcgaagccca gcaacgcatc
     1981 gtcaggcgca tcgccgccac ccttaatgta caggtgtcct tcgagcggct ggcccgcctt
     2041 tcgcacatgt cggacgtttc gctggaatcg tatgatgtgt ggctgcgcag ccaatggatc
     2101 acgcgtaact tcagcgccga ggagtggaat cgcgccgccg aaatgatcac catgagtatc
     2161 gagaaggcgc ccacgttctc ttcgttgtac agcggcttgg cacagatgaa caacgccgcg
     2221 cacattatgc agccgggcac gttccgcgac ccgaaaaaaa ttgcccgcac gctggcgctg
     2281 gcggaaaaag ccgtggcact ggatccactc gattcgcgtg cggagctttg ccttggctgg
     2341 gccctggcca tgagcgagcg ctacgcggcg gccgaaatcc acatggaact ggcgtgctcg
     2401 ttgaactcga acgactcgtg gacgatgatt tcgaccgcga tgttctatgc gttttgcggc
     2461 aatgccgaac gtgccgccaa cctttccgcg gaagcaatgg ggatgacgct gtcccccatc
     2521 cccaggcatt gggtttacga agcgacaatc cggcttcttc ggggtgacaa cgagggtgcc
     2581 atcgaagccg ccgatcgggc gcaagagaca ctgttgacgt tgcctgcgct gcgcgccgct
     2641 gccctggcca atctcggtcg taccagggaa gcccggctgg aggctgaacg tttctacgat
     2701 ggtgctcgtg ccaactgggt gaacgacgag gcgccgacca atcagacgat cgcacgctgg
     2761 ctgttgcaga tatttccgat cagcaagcgg gaagtctggg agcggttgcg ggacagcctg
     2821 agagaagcgg gtatccacgt cgagggcctg actttcgccg cacaggcgtt gacgaattag
     2881 cgcatgatcg cgctggggcg gtgcggtcct gacgcccgat gtccaggcgt caggtaaagt
     2941 gggcgcagat ttgcgctaca tgcaattgtt cagggtgtac tcaccgattc ggttcgcatg
     3001 cagttccaga atgccgatcc ggccggcggg ctggtggaac ggtccgctgt agagatcgcc
     3061 gtagaacggg cgaatctgat agggcgtgcc attcagaaga tcgtcttcgg atccctgcaa
     3121 ggtatccttt ggcggcaagc gcaggaccat ggtttcgacg ttgccctgga ctatcacaac
     3181 atgggtgtac tgcttcggca tcaccgtgac atcgacgttc gcggcagcca ctctcgtcat
     3241 gaactccttc gtggtcatgg ccgtcgcgaa gggcagcacg acggatcttc catcggcatc
     3301 cggaacaagc accggtccgc ccggtggcag gcaccacggc ttcggcttgc cctcgttatc
     3361 ggtgtccaaa gtcgtggtcg ctgccggcgg cgtggcggcg ccccaggtct tgttgaccca
     3421 gtagttgcgc ggcggctgag gcgcagttgg atcggttaca taatccagat gcgtagccca
     3481 gcttttgatc agccggcccc attgatccag gttcttgaca tcatagagtg gtgttggcgt
     3541 ccccattggc atgttttcct ttacgtgttg aacacggcga actgcttctt tcttgatgaa
     3601 tcgcagtcgt tgccagattg ggccaagccg aaattggccc tgcgtttcac catcctgtgc
     3661 gctataagcc gcaggctgcc cgaagttacg tgagactacc gtgagtcgaa tcacctatac
     3721 tgaagccgct gcttccgcca gggcacgtca tatctcggtt cggcctgttg ccgaacttgc
//
LOCUS       DASWQN010000331         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_560544, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000331 DASWQN010000000
VERSION     DASWQN010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560544"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VGC82_05445"
     CDS             complement(<1..462)
                     /locus_tag="VGC82_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422750.1"
                     /translation="MLREARRQKNLPTVQVPPVCLLDPDGDLVRTLRATGKAKPFADW
                     PCYHTELDTFSLAGQEVGIVGRVVGASFAVLVAEELFASGCRLLVSLTSAGQIMASGP
                     PPYFVVIDRALRDEGTSYHYAPPAEFAEADPPLVERAARATSKGKPRVVVGG"
     gene            571..1206
                     /locus_tag="VGC82_05450"
     CDS             571..1206
                     /locus_tag="VGC82_05450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0422751.1"
                     /translation="MSDTGRKAHWENVYRTKGEREVSWFQENPAPSLELIALAGLSAD
                     ASIIDIGGGASRLVDALVERNSGQITVLDLSAAALDAARERLGAQGAGAEWVIADVTT
                     WEPSKTYDLWHDRAVFHFLTGKGDQSAYVDRLKKAVKPGGCAIIGTFALDGPERCSGL
                     PVVRHDAASLSAVLGTDFKLIDARRHDHATPWGAVQRFQFSTFRRNQLRRV"
     gene            1466..>1687
                     /locus_tag="VGC82_05455"
     CDS             1466..>1687
                     /locus_tag="VGC82_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422752.1"
                     /translation="MSRQITIRRILSILVIAGLVLAPLSRPVMAETASDAITAAMSMS
                     VMADEMASDMPCCPTKAPAPVDCDKCVFMA"
BASE COUNT          334 a          482 c          536 g          335 t
ORIGIN      
        1 gccgccgacg accactcgtg gtttcccttt cgaggtggcc ctggccgcgc gttcgactag
       61 cggcgggtca gcctcggcga actcggcagg cggcgcgtaa tggtagctgg tcccctcgtc
      121 gcgcaacgcc cgatcaatga cgacgaaata tggcggcggt ccggatgcca tgatctgacc
      181 ggccgaagtg aggctgacga gcagccggca gccgctcgcg aacaattctt ccgcgaccaa
      241 aacggcgaac gaagctccta ccacccgccc aacaatgccc acttcttgcc cggcgagcga
      301 aaaggtgtcc aattcggtgt ggtagcaggg ccagtccgca aagggtttgg cttttcccgt
      361 cgcgcgaagc gtgcggacaa ggtcgccatc gggatccagc agacacaccg gcggcacttg
      421 aacggttggg aggttctttt ggcgtcttgc ctcgcgcagc aacgcagaag gcacgaagac
      481 ggaaggtgat gaagtgttct tgtggtccag tatcggaggc gtaatcatct caaggcgacc
      541 tggcggcgtt gcgggtgaag gagacatttt atgagtgata ccggcagaaa agcccattgg
      601 gagaatgtct acaggaccaa aggcgaaaga gaagtcagct ggttccagga aaatcccgcg
      661 ccatccctcg agctgatagc attggcaggt ctttccgccg atgcttcgat catcgatatc
      721 ggaggcggcg cgtctcgact cgtggacgcg cttgtcgaac gcaacagtgg ccagatcacg
      781 gttttggacc tttccgccgc cgctctcgat gcggccaggg agcgtctggg ggcgcaggga
      841 gccggcgccg aatgggtgat tgccgatgtc accacctggg agccgtcgaa aacttacgat
      901 ctctggcacg accgtgccgt gttccatttc ctgaccggta agggcgatca gtccgcctat
      961 gtcgaccggc tgaagaaagc cgtcaagccc ggagggtgtg ccatcatcgg gacgtttgct
     1021 ttggacggcc cggagagatg tagcgggttg ccggtcgtac gacacgacgc agcctccctg
     1081 tccgcggtgc tggggaccga tttcaaactg atcgacgcac gacgacatga ccatgcgaca
     1141 ccgtggggcg cggtgcagcg attccagttc agtacgtttc gtcgaaacca gctccggcgc
     1201 gtttgagcca gcagccacac gcggtaatgt gggccggggc tggatcattt cccaatccgt
     1261 catcacctgt ccgaagtgtg gaaaatggcg cggccggaga gcgtctcaag ccaaagcggg
     1321 agattgcgac gcaagtgact ttggttgttg aatttttttc tatcgtcagg cttgaccgga
     1381 cctgtgccaa aatgggcgca gccatcacgg cgtcgttagg ttcggcaggc tatcaatccg
     1441 agccgttctg tgggattttg gggacgtgag ccgccaaatc accatacgcc ggatcctgtc
     1501 tatccttgtg atcgcggggc tagttttggc tccgctgtcc cgtccagtca tggccgagac
     1561 ggcttcggat gcgataacag ccgccatgtc gatgtccgtt atggcggacg agatggcaag
     1621 cgacatgccc tgttgtccga ccaaagctcc ggcaccggtc gactgcgata aatgcgtgtt
     1681 catggcc
//
LOCUS       DASWQN010000332         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1352263, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000332 DASWQN010000000
VERSION     DASWQN010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352263"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VGC82_05460"
     CDS             <1..1093
                     /locus_tag="VGC82_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY0422753.1"
                     /translation="AITEARVSALTGSLLPPVRCAHHEEAIGRALDGLVGLGADLLLI
                     IGASAVVDRRDVGPAGIVRAGGEILHFGMPVDPGNLICLGRIGDKPALVLPGCARSPS
                     LNGIDFVLTRLFAGVAVTPRDMMRMGVGGLLKEMETRPMPREKATATPRSGAAPRSAP
                     VIAAVVLGAGRSRRMAPHNKLLVADKAGKPMIARVIDNVLSSNARPILVVTGHQADQI
                     EHALGGRPVRYVHAPDYAEGLSASLKAGIAAVPPECAAALVCLGDMPLVTGRMIDRLL
                     SMYDPTEGRLIVLPTFRGKQGNPMLWDRRFFAEILAISGDSGARFLVGKHTEAVCEVE
                     MADDAVLRDFDTTESLATLPRGMRPAGVE"
     gene            complement(1157..>2741)
                     /locus_tag="VGC82_05465"
     CDS             complement(1157..>2741)
                     /locus_tag="VGC82_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422754.1"
                     /translation="FTRSKFKRRDRPALRREAWIDVLVAVACILPLLLTAHLPLTDLP
                     NHLARQYILRDWSSSPFLQTFYYIRWELVPNLALELFVLAARHAMSIDMAVRLFCITT
                     VLMLFLGTRLVNVELSGGRARMYRFAPLLCYGGPFQYGFLSYCIGVGAGLLLFGVYLR
                     LRRRRGVMLVAFLLASGFVLMLCHLAAFGLFALAIGGCELASTFRTTGGAPRRLAAEL
                     AARLVVPVCCLVLVMLVFIGLGPATNQDAAVNAARFAFRFSSLHEKARSIYAITLFAS
                     PWLEALLLAMALAGLSAALLSRTVRFHPVGLTVLAVMAIAWMALPNIALGSAFIDYRI
                     PWAMSFFLLSGLVPGPGSQRLRKPLGQYFSLLSAFRIGLISVLWLRWEPTLTALDGVL
                     DNVPVGTRIKVVEGQLPTGKLFRQPDFSNVALYAVARRQAFEPEVFANVAGQILHFQP
                     HFEQLWEQDDFAREIPSRLNYLAPDYDYVLVLLPEFAQISPLLPLTCQQSGPNFELLK
                     VNRSGAILSPDTDQSGHCS"
BASE COUNT          558 a          845 c          899 g          439 t
ORIGIN      
        1 agcgataacc gaggctcggg ttagcgcgct gacgggctcg ctgttgccgc cggtgcgctg
       61 cgcgcatcac gaggaagcga tcggccgggc gctggatggg ctggtcggtc tgggcgccga
      121 tctcctgctt attatcggtg cctccgcggt tgtggaccgg cgcgacgtgg ggccggccgg
      181 catcgtgcgt gccggcggag aaatcctgca tttcggcatg ccggtcgatc cgggaaacct
      241 gatctgcttg ggccggatcg gtgataaacc cgccttggtg ttgccgggat gcgcccgcag
      301 tcccagcctg aacggcatcg acttcgttct gacccgtctg ttcgccggcg tcgccgttac
      361 gccgcgcgac atgatgcgca tgggcgtcgg cggcctgctg aaggagatgg agacccggcc
      421 gatgccgcgc gagaaggcga ccgcgacgcc taggtccggt gccgcgccgc gcagcgcacc
      481 agtgatcgcc gcggtggtcc tgggcgccgg gcggtcgcgg cggatggcac cgcacaacaa
      541 gctgctggtc gccgacaagg ccggaaagcc gatgatcgcc cgcgtcatcg acaacgtcct
      601 gtcctcgaat gcccgcccga ttctggtcgt gaccggccat caggcggatc agatcgagca
      661 tgcgctcggc ggccggccgg tgcggtacgt gcatgcgccg gattatgccg agggtctgtc
      721 cgccagcctg aaagccggta tcgccgcggt gccgccggaa tgtgccgccg cgctggtgtg
      781 cctgggcgac atgccgctgg tgaccgggcg gatgatcgac cggctattgt cgatgtacga
      841 cccgaccgag ggccggctga tcgtactgcc aacctttcgc ggcaagcagg gcaacccgat
      901 gctgtgggac cggcgcttct ttgccgaaat cctggcgatc agcggcgact caggcgcgcg
      961 gtttctggtg ggaaaacaca ctgaggccgt ttgcgaagtc gagatggccg acgatgccgt
     1021 gctgcgggat tttgacacca cggaatccct ggccacgctg ccgcgcggca tgcgtccggc
     1081 gggtgtcgaa tagttcgggc tctgggcgat cgcgggctca ctggaagcgt cggcagcacg
     1141 accatggctt ccggcgtcaa gagcaatgac cgctttgatc ggtgtccggg gacaggattg
     1201 ctcctgaacg gttcaccttt agcagttcga agttcgggcc gctttgctgg caggtcagcg
     1261 gcaacagcgg cgaaatctgg gcgaattccg gcagcagaac cagaacgtag tcgtagtcgg
     1321 gcgcgaggta gttcaacctg gacgggatct cccgggcgaa gtcgtcctgc tcccaaagct
     1381 gttcgaagtg cggctggaaa tgcagaatct gccctgcgac attggcaaaa acctctggct
     1441 cgaatgcctg ccgtcgtgcc acggcgtaca acgcgacgtt gctgaaatct ggctgacgaa
     1501 ataatttacc ggtcggcaac tggccttcga cgaccttgat ccgcgtcccc accgggacat
     1561 tgtcgagcac cccgtcaaga gcggtaagag tgggttccca cctgagccat aggaccgata
     1621 tcagcccgat acgaaaggcc gacaacaggc taaaatactg gcccaaaggc tttcggagtc
     1681 gctgcgaacc cggccccggc acaagcccgg agagcaggaa gaaggacatt gcccagggga
     1741 ttcggtagtc gataaacgcc gaacccaggg cgatgttcgg caacgccatc caagcaatgg
     1801 ccatcaccgc gaggacggtc aacccgacgg ggtgaaagcg caccgtcctg ctcaggagcg
     1861 ccgccgacaa gcctgccagc gccatcgcca gcagcagggc ctccagccat gggctggcaa
     1921 acaatgtaat ggcgtagatc gaccttgcct tctcgtgcag cgaactgaag cggaaagcga
     1981 accgggcggc gttgaccgcc gcatcttggt tcgttgccgg tccaagcccg ataaacacca
     2041 gcatgaccag cacaaggcaa cagaccggca ccaccaaccg ggccgccagt tccgcggcca
     2101 gacgacgggg tgcgccccct gtggtcctga acgtgctggc gagttcacag ccgcctatag
     2161 ccagggcgaa cagtccgaac gcggcgaggt ggcaaagcat caggacgaaa ccggatgcca
     2221 gcaggaaggc tacaagcatc acaccccgcc tgcggcgcag acgcaaatag acgccgaaaa
     2281 gcagcaggcc ggcgcccacc ccgatgcaat aactcaggaa cccatattga aacgggccgc
     2341 catagcaaag cagtggcgca aagcgataca tccgggcccg tccgccgctg agttcgacgt
     2401 tcaccagccg ggtccccagg aacagcatta gaacggtggt gatgcaaaac aaccgcactg
     2461 ccatgtcgat tgacatggcg tgacgggccg cgaggacgaa cagctccagg gcgaggttgg
     2521 ggacgagctc ccaccggatg taatagaaag tttgaagaaa cgggctgctc gaccagtccc
     2581 ggagaatata ctgacgcgcg agatgattgg gcagatcggt cagcggcaga tgcgcagtca
     2641 gcaacagcgg cagaatacac gccacagcga ccagcacatc tatccaggct tcgcgtctca
     2701 gtgccggacg atcgcgtcgc ttaaatttgg accgggtgaa a
//
LOCUS       DASWQN010000333         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1352273, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000333 DASWQN010000000
VERSION     DASWQN010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352273"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..569)
                     /locus_tag="VGC82_05470"
     CDS             complement(60..569)
                     /locus_tag="VGC82_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0422755.1"
                     /translation="MPELNDLRAQRDNLEQMIEARQRQLGSDHPDLANDLRDLGRLSH
                     ELGDMMDAQAQLRRALALHTEALGPEHPEAATDINHLGLILHDLGDLDGAYAAFERAL
                     AIDETALGQDHPSVARDANNLAGVLKDMGDGFSAREALDWSLSIYRKAFGPDHPTTKA
                     VARNRAGLG"
     gene            complement(595..>1728)
                     /locus_tag="VGC82_05475"
     CDS             complement(595..>1728)
                     /locus_tag="VGC82_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0422756.1"
                     /translation="ELVQAKKMEGLGKLTGGIAHDFNNLLTPILITLDLLQRRYLQDE
                     RALRMTKSALSAAERARVLITRLLAFARRQHLHAQPVDLAALVRSKLDFITRSVGQSV
                     AVSVSIADDLPPARVDRTQLEMALLNLCLNARDAMPQGGNLTLSGDEVMVQPEAASNL
                     VAGRYVRLSVSDSGFGMDQETLLRAAEPFYSTKEVGKGTGLGLSMVHGLAAQSGGALV
                     LESTRGHGTTATIWLPVDPSAAPIEEVVEPDPAAAGRPLSVLLVDDDELVRFAASEML
                     HDLGHSAIQASCGAEAVEALRRHADFDLLITDYLMPGMTGVDLAQVARKARPDLPVLL
                     VTGYANIEDVDGGGLPRLAKPFGTHELSNAIAGILSRGLAPAR"
BASE COUNT          306 a          585 c          526 g          311 t
ORIGIN      
        1 ccgcgtttcc agccagcacg gaaccactcg cctttggcgc gccgccctca aacccgtttc
       61 tatcccagcc cggcccgatt gcgcgcaacg gctttggtcg tggggtgatc gggaccgaaa
      121 gccttccggt agatcgacag cgaccagtcc agcgcctcgc gggccgagaa cccgtccccc
      181 atgtccttca acaccccggc caggttgttg gcgtcacggg caacgctggg atgatcctgg
      241 cccagcgcgg tttcgtcgat cgccagggct cgctcgaacg ccgcataagc cccgtccagg
      301 tcgccgaggt cgtgcaggat caagccgaga tggttgatat ccgtggcggc ttccggatgt
      361 tccgggccca gcgcttcggt atgcaaagcc agcgcgcggc gcaactgcgc ctgggcgtcc
      421 atcatgtcgc caagctcgtg cgacaggcgc ccaagatcgc gcaggtcatt ggcaaggtcc
      481 ggatggtccg aaccaagctg ccgctgccgc gcttcgatca tttgctcaag gttgtcgcgt
      541 tgtgctcgca ggtcgtttaa ttcgggcatg gcttgggtcc caacagcacg ccgtctatct
      601 ggccggcgcc aagccgcggc tcaagatccc ggcgatggca ttcgacagtt cgtgagtgcc
      661 gaacggcttg gcaagccgag gcaacccgcc gccgtccaca tcctcgatgt tcgcgtatcc
      721 ggtcaccagc aagaccggca ggtcgggacg cgccttgcgc gccacctgag ccaaatccac
      781 gcccgtcatg cctggcatca gatagtcggt aatcaacagg tcgaagtcgg catgccggcg
      841 aagcgcttcc acggcctccg ccccgcatga ggcctggatc gccgaatgtc ccagatcgtg
      901 caacatttcg ctggcggcaa agcgaaccag ttcgtcgtca tccaccagca agaccgacaa
      961 gggccgcccg gccgcagctg gatccggctc gacgacctct tcaatcgggg cagcgcttgg
     1021 atcgaccggc agccagatcg tagcagtcgt cccatggcca cgggtgcttt ccagcaccaa
     1081 cgcgccgccc gactgcgcgg ccagtccatg caccatggat agccccaggc cggtgccctt
     1141 gccgacttcc ttggtcgagt agaacggttc agccgcgcgc agcaatgtct cctgatccat
     1201 gccgaatccg ctgtcggata ccgacaagcg gacgtatcgg ccggcgacga gattgctggc
     1261 cgcttccggc tggaccatta cctcatcgcc ggagagcgtc aggttgcctc cttgcggcat
     1321 ggcgtcccgc gcattcaggc acaaattcag cagcgccatt tccaactgag tccggtccac
     1381 ccttgcgggc ggcaggtcat cggcgatgct gacgctcacc gcgaccgatt ggccaaccga
     1441 gcgcgtgatg aaatcgagct tgctccggac cagggcagcg agatcgacgg gctgcgcatg
     1501 aagatgctgg cgcctggcga acgcgagcaa cctggtgatc aggaccctgg cgcgttccgc
     1561 cgcggaaagc gccgacttcg tcattcgcag cgcccgttcg tcctgcagat agcggcgttg
     1621 caacaggtcc agcgtaatca ggatgggcgt cagcaggttg ttgaaatcgt gggcgatgcc
     1681 gccagtcagt ttgcccaggc cttccatctt tttcgcctgg accagttc
//
LOCUS       DASWQN010000334         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2144174, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000334 DASWQN010000000
VERSION     DASWQN010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144174"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VGC82_05480"
     CDS             <1..372
                     /locus_tag="VGC82_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0422757.1"
                     /translation="ANARNAGNLSGGARNASLVHMTKTLAVQMGRHGITVNCIHPGTT
                     RTERTPSLLAARAAQLGISPEEAERQDFAPDSPRGNAICRMVDAREVAFVTVFLASDK
                     AWAVSGELVAATGGAGRTVHY"
     gene            493..1320
                     /locus_tag="VGC82_05485"
     CDS             493..1320
                     /locus_tag="VGC82_05485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0422758.1"
                     /translation="MAVIDVQVHPFDRNHPGRPWASPSHGLDSATGEEMVAAMNNVGV
                     DGAIMVSSFSAYEYDPSYALEVYHTYPDKFRVVTPVDATDPAIDDVVAKWAATQGARG
                     IRIRMRDGLPMDADHPGLNRAFAAAAKHGLPVNLLCWGILDKGLPHIQRHPDTVIVID
                     HLGLLQPARPPVPADVWADLPKLLALSRYENVRVKISGACTMSHQPFPYDDIWEPVLR
                     VIDAFGLDRCMWGTDWTRTIGMLTYEQGVAPFRDTKRLSDSDKAALMGGTLQKIYKW"
     gene            1378..2313
                     /locus_tag="VGC82_05490"
     CDS             1378..2313
                     /locus_tag="VGC82_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEY0422759.1"
                     /translation="MALLIVDLDKHIGNADAWRDTFGEKLPDLEIRTWPEAGNLADIE
                     YLAFMHPDFDSLPVFPNLKAMFSRAAGVESFVQHPKLPKVPLCKVEPPGGDPMMTEYV
                     VMHVLRFHRDMPAYQAAQAKREWRRTTILRPEQRRVGFLGFGMMAKAPALVLKSLGFK
                     VSAWVRNPRNESDVPIYHGPDQLGPFLQQSDIAVCLLPLTRETEGIFCTRTFAMMPRG
                     AMLVNVGRGKHVVDEDLIEALDSGQLSYAALDALWPEPLPPTSPLWLHPKVTVMPHVA
                     RRPTVAQLVSEIAANIRSIQAGGRPLQEVDITEGY"
BASE COUNT          487 a          763 c          785 g          479 t
ORIGIN      
        1 gccaacgctc gcaacgccgg caatctaagc ggcggtgcgc gcaacgcctc cctcgtgcac
       61 atgaccaaga cgctggcggt ccaaatgggc cgccatggca tcaccgtcaa ttgtatccat
      121 cccggcacga cccgtaccga acgcacgccg agcctgctcg ctgcccgcgc cgcgcagttg
      181 ggcatatccc cggaagaagc ggagcggcag gattttgccc ccgactcacc gcgcggcaac
      241 gccatctgcc gcatggtgga cgcccgcgag gtcgcattcg tcacggtctt cctggcctcg
      301 gacaaggcct gggcggtctc cggcgagttg gtcgcggcaa ccgggggagc cgggcggacg
      361 gtccattact aggccgcact catataatcc cgttgacagc ggccagagcg tcccacccgt
      421 cgccgtgccc gtcactctgg gcagtccggt cgcgatagcg gataaatggc tcgaaccaac
      481 aggaggaccc ccatggcagt gatcgacgtc caggttcatc cctttgatcg aaatcatccc
      541 ggccggccgt gggccagtcc gtcgcacgga ctcgactccg ccacgggcga ggagatggtc
      601 gctgccatga acaatgtcgg cgttgacggc gcgatcatgg tgtcgtcgtt cagcgcctat
      661 gaatacgatc cgagctatgc gctcgaggtt taccacacct atccggacaa gttccgggtc
      721 gtgacgccgg tcgatgcgac cgatccggcc atcgacgatg tggtggcgaa atgggcggcg
      781 acgcagggcg cgcgcggcat tcgcatccgt atgcgtgatg gcctgccaat ggatgccgac
      841 catcccgggc tgaaccgcgc tttcgccgcc gcggccaaac atggtttgcc tgtcaatctg
      901 ctttgctggg gcatactgga caaaggcctg ccgcacatcc agcgacaccc cgatacggtc
      961 atcgtgattg accatctcgg tctgctgcaa cccgctcgcc cgccggttcc ggccgacgtc
     1021 tgggctgatc taccgaagct gctcgctttg tcgcgatacg agaatgtaag ggtgaagatc
     1081 agcggcgcct gcacgatgtc gcaccaaccg ttcccgtacg acgacatctg ggagccggtg
     1141 cttcgggtca tcgatgcttt cgggctggat cgctgcatgt ggggcaccga ctggacgagg
     1201 acaatcggca tgctgaccta tgaacaaggc gtggcgccgt tccgcgacac caaacgcctg
     1261 tccgacagcg ataaggcggc gctcatgggc ggaacgttac agaaaatcta caaatggtga
     1321 tgcgaccgcg acgattcggc cacggcacgc ttcggcggaa taactcagga ggcggtcatg
     1381 gcgttgctca tcgttgatct cgacaaacat atcggcaatg ccgatgcatg gcgcgacacg
     1441 ttcggggaaa agcttcccga tttggaaatc cgcacttggc ctgaggccgg aaacctggcg
     1501 gatatcgaat atctcgcctt tatgcatccc gatttcgatt ctcttcccgt ttttccgaat
     1561 ttgaaggcca tgttcagccg cgccgcgggt gtggagtcct tcgttcaaca tccaaagctg
     1621 ccgaaggtcc cgctatgcaa ggtcgagccg ccaggcggcg atccaatgat gacggaatac
     1681 gtcgtcatgc acgttcttcg ttttcatcgc gatatgcccg cgtatcaggc ggcacaggcc
     1741 aagcgggaat ggcgccgcac cacgatcctg cggcctgagc aacggcgtgt cggattcctc
     1801 ggcttcggca tgatggcgaa ggcgccggcg ctcgtgttga aatcgctcgg tttcaaggtc
     1861 tcggcgtggg ttcgcaaccc gaggaacgag agtgacgttc cgatctatca tggtcccgac
     1921 caacttggcc cgtttcttca gcagagcgac attgcggtgt gcctgttgcc gcttacgcgg
     1981 gaaaccgagg ggatattctg cacgcgcacc tttgccatga tgccacgcgg ggcgatgctg
     2041 gtgaatgtcg ggcgggggaa gcatgtggtg gacgaggatc tgatcgaagc gctcgattcg
     2101 ggacagttgt catacgcggc gttggacgcg ttgtggccgg agcccctgcc gccaacgagc
     2161 ccgctctggc tgcaccccaa ggtcaccgta atgccgcacg tggcgcggcg gccgaccgtt
     2221 gctcagctcg tgtccgagat cgcggcgaat ataagaagca ttcaggcggg cgggcggcct
     2281 ttgcaggagg tcgatataac ggagggatat tagagagtga tcacgccggg cggaccgttc
     2341 gaagcgtgtg aaccaagcga tcaaacaaaa tgctctagcc ccgcctgctg gtagctgggt
     2401 gggcggggcc cttgcttcag cccgtggttt cagtttgaaa gagacttatc cgaccattgg
     2461 atcaatcaac aacgccaggg gtgtgactat gcgaccgaac aagatcaagc agat
//
LOCUS       DASWQN010000335         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1616328, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000335 DASWQN010000000
VERSION     DASWQN010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616328"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..1665)
                     /locus_tag="VGC82_05495"
     CDS             complement(283..1665)
                     /locus_tag="VGC82_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0422760.1"
                     /translation="MSASTLPNATLTEAYQSLTPGSAALAAKARTLMPSGLSHDSRHF
                     DPYPLYIRRALGPVKWDVDNNRYVDYFGGHGALILGHNHPTVMAAVHAALDNGTHFGA
                     CHELELRWAELITQMVPSAERVRFTSSGTEATLLALRLARAYTGRPKLVRFRGHFHGW
                     HDHMTSGHTSHFDGTPTSGVISAVAENVLLCDPNDVAGITRIFNDHKDIAAVILEPTG
                     ANFGKMPILPSFLTLLRDLTKQAGTILIFDEVVTGFRVSPGGLQATLGITPDMTTLAK
                     ILSGGLPGGAVVGRKDIIDWLDFEVAKAAGKEKVAHLGTFNANPVSAAAGIATLQILA
                     TTDACARANAFGDQVRARMNAVLEEEGVKWAVHGSYSGMHIYTNPEGEDIVPGAFDAA
                     QYIQKMIDKPRGEGVTGQVRMGLLVNGVDMNSGPSGTISAMHGEEELAITVDAFRATL
                     RALAREGVLS"
     gene            complement(1677..2438)
                     /locus_tag="VGC82_05500"
     CDS             complement(1677..2438)
                     /locus_tag="VGC82_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY0422761.1"
                     /translation="MSGIVKRFGTNEVLRGVSLRVARGETVVVIGPSGSGKTTLLRCV
                     NMLEDYQAGTVAVDGEPVGYAVDREGRRQRMAEREIAASREKIGIVFQSYNLFPHMTA
                     LQNIVAAPIRVRGIPRPKAEARARDLLAMVGLSDKADAYPARLSGGQQQRVAIARALA
                     MDPKIMLFDEVTSALDPELVGEVLAAMQQLARDGMTMIVVTHEMSFARDVADKVVFMD
                     GGVIVEEGAPEEMFTSPRTDRVRQFLKRYNDRYRL"
     gene            complement(2491..3144)
                     /locus_tag="VGC82_05505"
     CDS             complement(2491..3144)
                     /locus_tag="VGC82_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0422762.1"
                     /translation="MKTFAPLLHSYWYLFHGAGMTLLMASASVVPATILGAMLALAQI
                     FASRFVRSAIVCYIFVIRGIPLLVLLTFVYYLLPLTGIDLPPFWGVVLVLSCYYAAFM
                     SEVFRAGILSLPQAQWDAARSLGMSRPLMLRIVIFPQALRLAAPPFVNLCINLIKATS
                     LVSIVGLWELTMASREVVERTLAPFQIFLAAAGMYFCICYALALYGKYLERRILVAH"
BASE COUNT          671 a         1095 c          937 g          583 t
ORIGIN      
        1 ccggtgccat ttgttccatg tgcggcagat gatgtgaaac ctgggtcacc ggcaggccct
       61 tcaggttcag cgcgatccgc acgcgccaag ccgcggagct gcggaaaaag ccatgcagga
      121 cggacgggct gttagcagca ttcacggctg acatctcccg gctgatagtt gctgttctcg
      181 tttcccctcg gccactccag aacgtgccac aaccgttcag gacgactggc gccgacgtcc
      241 gatcgggcgt ttcaaccacc gtcctggtct tttcggcaaa catcacgaca ggacaccttc
      301 ccgcgccagc gcccgaagcg tcgcccgaaa cgcgtcgacc gtgatcgcca gctcctcttc
      361 gccatgcatc gccgaaatcg tcccggacgg gcccgagttc atatccaccc cattcaccag
      421 caatcccatc cgcacctgtc cggtgactcc ctcgcccctg ggcttgtcga tcatcttttg
      481 aatgtattga gccgcatcga atgcgccggg aacgatgtct tccccttcgg gattggtata
      541 gatatgcatt ccggagtagg atccgtgcac cgcccacttc acgccttcct cctccagcac
      601 cgcgttcatc ctggcgcgca cctggtctcc gaaggcgttc gcccgagcac aggcgtccgt
      661 tgtggccagg atttgaaggg tcgcgatgcc ggccgctgcc gagacgggat tggcattgaa
      721 ggtcccgaga tgggcgactt tctccttgcc cgccgccttg gcgacttcaa agtcgagcca
      781 gtcgatgatg tccttgcgcc cgaccaccgc accacccggc aacccgccgg acaggatttt
      841 tgccagcgtc gtcatatccg gcgtgatccc cagcgtcgcc tgaaggccac cgggagacac
      901 ccggaacccg gtcacgacct cgtcgaagat caagatcgtt ccagcctgct tcgtgaggtc
      961 acgcagaagt gtcaggaacg acggcaggat cggcatcttg ccgaaattcg ccccggtggg
     1021 ctccaggatg accgcagcga tgtccttgtg gtcgttgaat atacgcgtga tcccggcaac
     1081 atcgttcgga tcgcacagca gcacgttttc ggcgaccgca ctgatcacgc cgctggttgg
     1141 agtgccgtcg aaatgactgg tatggccgct ggtcatgtgg tcgtgccagc catggaaatg
     1201 accgcgaaag cggaccagct tcggccgccc cgtgtaggcc ctggccagcc gcagcgccag
     1261 caaggtcgct tcggtccccg acgaggtaaa ccgcacccgt tcggctgacg gcaccatctg
     1321 cgtgatcaac tcggcccagc gcagttccag ttcatgacag gctccgaaat gcgtgccgtt
     1381 atccaacgcc gcatgtaccg cagccatcac cgtcggatgg ttgtgtccca ggatcagcgc
     1441 cccatggccg ccaaagtaat cgacgtatct gttgttatcg acatcccact tcaccggccc
     1501 cagcgcccgc cggatataca gcggatacgg atcgaaatgc cggctgtcat gcgacagacc
     1561 gctcggcatc aacgtccgcg ccttcgccgc cagggcggcg gagccgggcg tcagggactg
     1621 gtacgcctcg gtcaaggtcg cgttcggcag agtgctggcg gacatgaggg aacccttcaa
     1681 agtcgatatc ggtcgttata gcgcttcagg aactggcgca cacggtcggt gcgcggcgag
     1741 gtgaacattt cctctggcgc gccttcctcg acgatgacgc cgccgtccat gaacacgacc
     1801 ttgtcggcca catcgcgcgc aaaactcatt tcgtgggtaa ccacgatcat ggtcatgcca
     1861 tcccgagcca gttgctgcat cgcggccaga acttccccga ctagctcggg atcgagcgcc
     1921 gaggtcacct catcgaacag catgatcttt gggtccatcg ccagcgcccg ggcaatcgcc
     1981 acgcgctgct gctgaccgcc ggacagacgc gccggataag cgtccgcctt gtcggataac
     2041 ccgaccatcg ccagcaggtc gcgcgcccgc gcttccgctt ttggccgagg gatcccccgc
     2101 acccggattg gtgccgcgac aatgttctgc agtgcagtca tatgcgggaa cagattgtag
     2161 ctctggaata caatgccgat tttctcacgc gacgcggcga tttcacgttc cgccattctt
     2221 tggcgccggc cttcgcgatc cacggcgtaa cccaccggtt ccccatcaac cgcaaccgtg
     2281 cccgcctgat agtcttcgag catgttcacg catcgcagca gcgtcgtttt ccctgatccg
     2341 ctgggcccga tgaccaccac cgtttcgccg cgtgcgaccc tcagcgacac gccgcgcaac
     2401 acctcgtttg tcccaaagcg tttcacgatg ccagacatct cgagcgcggg acgcgcaatg
     2461 ggcaatccgg ggggcagtgc ggggggcaac tcagtgggca acgaggattc ggcgttcaag
     2521 gtattttcca tacaaggcca gtgcatagca gatgcagaaa tacatccccg ccgccgcgag
     2581 gaaaatctgg aacggcgcca gcgtgcgttc gaccacctcg cggctggcca tcgtcagttc
     2641 ccacaatccg acgatggaaa ccagagacgt agccttgatc aggttgatgc aaagattcac
     2701 gaacggcggt gccgccagcc gcagagcctg cgggaaaatc acgatccgca acatcagcgg
     2761 ccgggacatg ccgaggcttc tggccgcatc ccactgtgcc tgcggcaatg acagaatgcc
     2821 ggcgcgaaac acctcgctca tgaacgccgc atagtagcag gacagcacca gaaccacccc
     2881 ccagaacggc ggcaggtcga taccagtcaa cggcagcagg taatacacga aggtcagcag
     2941 aaccagcagc gggattccgc gaatgacgaa gatgtagcag acgatcgccg aacgaacgaa
     3001 ccgcgacgcg aagatctgcg ccagcgccag catcgcgccc aggatagtcg ccggaaccac
     3061 ggaggccgaa gccatcagca gcgtcatacc ggcgccgtga aacaaatacc agtagctgtg
     3121 caaaagcggg gcaaatgtct tcaagacacg aacgccagtt ggcgctcggc gacgcgggca
     3181 cccaggctga tgacactcgt cagcaccagg tagatcagcg ccaccgcaaa gaacacgtcg
     3241 taggtattcg ctgaggactc cacccagacc tgcgccacct tcatca
//
LOCUS       DASWQN010000336         5562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_561027, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000336 DASWQN010000000
VERSION     DASWQN010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561027"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VGC82_05510"
     CDS             <1..369
                     /locus_tag="VGC82_05510"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0422763.1"
                     /translation="LYGDGSQTRAFCFVDDLVEGFIRMMATGPEVTGPVNIGNPHEIP
                     VSELAERVIRITGSASRIVHRPLPQDDPLQRCPDITLAKNILGWQPTIELDKGLRRTA
                     AYFQQMLAESKERDLQITGG"
     gene            435..1412
                     /locus_tag="VGC82_05515"
     CDS             435..1412
                     /locus_tag="VGC82_05515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0422764.1"
                     /translation="MAVIILHWNDEAATLACLNELRSTDYTNYSTIVVDNGSTTGSAE
                     RIGLAGQVDLMTNPTNLGYTGGVNVGIGRAMAAGADYVWLLNSDAATRPDVLSLLVAA
                     AEADQRIGLVSPVFHDPERPDVAEFCLGRFDPASRYATQTADPAIAREWQQNFPDQIL
                     VLGTALLIRRRLIETIGTLDPTFFAYVEDVDYSLRALAAGFRNVVVPEAVVLHKFKQP
                     TDNPGGVPAYLHYFITRNYLLLWRKLPRPMLMRRAALWFLHQRLTQIAKMRDVPAAID
                     AVLAGLWDGVRGIGGPYRPGRKAPWLLRATLGRYPAFFLAVLDGGKQAR"
     gene            1409..2557
                     /locus_tag="VGC82_05520"
     CDS             1409..2557
                     /locus_tag="VGC82_05520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0422765.1"
                     /translation="MNLSPRIAVACSGLGHVQRGIESWASDLAQALRRTGADVTLFSG
                     ASLDGAIALPTFRRTGRTAVALATGARRLGGWRYGMGSPYDVEQTSFAVALWQRISSG
                     FDILHVQDPMIAIWLQRAHCAGLSRPKVIYANGTGETASVMRRFDALQFLTKAAADSW
                     VPQKPSGQAIFTIPNFIDTKRFSPGDQAASRARFGLPRDRTIILCCAAIRRFHKRIDV
                     LLSEFANACRRTDAMLLIAGGRESDTDALIAEGSALLGERVRFLPDLPRDAMPDLYRA
                     ADAFVLPSLHEMFGIVLLEALATGLPVLCNDTQDFRAIAGPGGRYGDFGARDGLARGL
                     TDLLDPMLRATTARAARAHVERHFSEAAVMGSITAMYRSVHAASSHVR"
     gene            2547..4562
                     /locus_tag="VGC82_05525"
     CDS             2547..4562
                     /locus_tag="VGC82_05525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0422766.1"
                     /translation="MSDDRQGTVSVIIPAYNAAAYISRAIESALAQTHAPLEILVIDD
                     GSSDATPEIVGRLPVPVRLVRKSNGGPASARNLGASLARGEWLAFLDADDWWFPEKLR
                     VQLEYATGPEIGMLHCLPDHRDDDVPPLLSFEHMWDRNWIINSSVLIRRVAFNALGGF
                     NESRALISVEDYNLWIRAAASGWKVVTCPRVLVHYTRGIGISSNPGRFMQASLYNVDD
                     IGGRLSLPLATIKRKRNEIIFDFGRKALFERDLGTARRLLRLAFKERPNARNLLHMGV
                     AAMPVPVLDARRSLLRFLEPGNAAQDDARPSASKPEGQPETAPRFASILDPARHAAAR
                     EQRLDRPMLITTIDAEEDFDWTRPFSRAATDVKSIRSQHRAHRVFERYGVVPIYMVDH
                     PVASQDAGRAPLRELLRSGACDIGAQLHPWVTPPFVEDVSTHNSYAGNLPRPLEFAKI
                     KVLTHELAQAFGQPPRMFRAGRYGAGRNTGGILSQFGYEADSSVVPCWSFAAQGGPDF
                     RRVNASPYWIDAERRLLELPVTAAIVGRAAGLPDALTSRFFGRLSEATALPSIMSRLG
                     LLERIKLTPEGITIEEAKRLVRHMVRLGHKVFVLTYHSPSLEPGNTPYVRSEADLAKF
                     LRWLDEFYDYFTREIGGVCATWREVRSALEQDHAGAVSAHVGSQTSP"
     gene            complement(4661..>5562)
                     /locus_tag="VGC82_05530"
     CDS             complement(4661..>5562)
                     /locus_tag="VGC82_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY0422767.1"
                     /translation="FDIRRGETLSLVGESGCGKSTVGKAILRLFKLTEGEVYLDGERI
                     DNMAAGKLRMMRKRVQVVFQDPFSSLNPRMKVRDILAEPLINFGMAHGRRDLTAKVEA
                     LMDKVRLPRDAINRFPHEFSGGQRQRIGIARALAPGADLIICDEAVSALDVSVKAQII
                     NLLADLQDELGLALLFISHDLATVEYLTHRVAVMYLGKIVELTDRRTLFAQPHHPYTR
                     ALLSAVPVPDPTAKRQRIILKGDVPSPINPPSGCRFHTRCPLAFDRCRSEEPLLRSVG
                     EGHLSACHLDEVPGGAVVPLAAE"
BASE COUNT          983 a         1857 c         1695 g         1027 t
ORIGIN      
        1 ctttacggcg acggcagcca gacccgggca ttctgcttcg tcgatgacct ggtcgaagga
       61 tttatccgga tgatggcaac cggtccggag gtcacgggtc ccgtcaatat cggtaatccg
      121 catgaaattc cggtcagcga actggcggaa cgggtgatcc gcatcaccgg gtcggcgtcg
      181 cgcatcgtcc atcgcccgct gccgcaggac gatccgctgc aacgctgccc cgacatcaca
      241 ttggcaaaga acattctcgg atggcagccc accatcgaac tcgacaaagg gttgcgcagg
      301 acggcggcct acttccagca aatgctggca gaatcgaagg agcgggacct gcaaatcacc
      361 ggcggataag gatgaaccga gcggccgatc ctcgccctcc ggttggttca gcgatccagg
      421 tggttcggcc caacgtggca gtcatcatct tgcactggaa cgacgaggcc gccacgctcg
      481 cctgcctcaa cgagctacgc tcaacagact acacaaacta cagcacgatc gtcgtggaca
      541 acggatcgac caccggttcg gctgaacgta tcggcctcgc cggccaggtc gatctcatga
      601 cgaatccgac gaacctgggc tacacagggg gcgtcaatgt tggcatcggg cgggcgatgg
      661 cggcgggcgc cgactatgtc tggttgctga acagcgacgc cgccacccgg ccggatgtgc
      721 tgtcgcttct ggtcgcagcc gcggaagccg accagcggat cggccttgtc agccctgtct
      781 tccatgatcc ggaacgtccc gatgtcgccg aattctgtct cggccgcttc gatccggcgt
      841 cccgctatgc cacgcaaacc gccgatccgg ccatcgctcg ggaatggcaa cagaatttcc
      901 cagatcagat cctggttctc ggaacggccc tgctgatccg ccgccgcctg atcgagacga
      961 tcggcaccct cgaccccacc ttcttcgcct atgtcgagga tgtggactac tcgctgcggg
     1021 cgcttgccgc cggcttccgc aacgtcgtcg tccccgaagc agtggtcttg cacaaattca
     1081 agcagccgac ggacaacccc ggtggcgttc ccgcttatct gcattacttc attacgcgca
     1141 actatcttct gctgtggcgc aaactgccgc ggcccatgct gatgcgcagg gcggcattgt
     1201 ggtttttgca ccagcgcctg acgcaaatcg ccaaaatgcg agacgttccc gccgcgatcg
     1261 atgccgtgct ggccggtttg tgggatgggg tccgtggcat cggtggcccg taccggcccg
     1321 gccgcaaggc accatggctg ctgcgtgcca cgctcgggcg gtatccggct tttttcctgg
     1381 ccgtgctgga cggcgggaaa caggctcgtt gaacctgtcg ccgcggatcg ccgttgcctg
     1441 ttcaggcttg ggtcatgtcc agcgcggcat cgaatcctgg gcctccgacc tggcgcaagc
     1501 cttgcgccga acgggcgccg acgtgacctt gttcagcggc gcatccctcg acggcgccat
     1561 tgccttgccc accttccgcc ggactggacg gaccgccgtc gcgttggcga ctggcgcccg
     1621 gcgcctcggc ggctggcgtt acggcatggg gtcgccctat gacgtggagc agaccagctt
     1681 tgccgtcgcg ctgtggcaac ggatcagcag cgggttcgac attttgcacg tccaggaccc
     1741 gatgatcgcc atctggcttc agcgcgcgca ctgcgccgga ttatcccggc caaaggtcat
     1801 ctatgccaac ggcaccgggg aaaccgccag cgtgatgcgc cgcttcgatg cgctgcaatt
     1861 tctgaccaaa gcggccgccg actcctgggt gccccaaaaa cctagcggcc aagcgatatt
     1921 cacgatcccg aactttatcg atacgaagcg cttttccccc ggcgaccagg ccgcgtcccg
     1981 cgcccggttc ggattgccac gcgaccgcac catcatcctg tgctgcgccg ccatccgccg
     2041 cttccacaaa cggatcgacg ttctgctgag cgagttcgcc aacgcgtgcc gccgcaccga
     2101 cgcgatgttg ctcatcgccg gcggccggga atccgacacg gatgccttga tcgccgaggg
     2161 ctccgccctg ttaggcgagc gcgtccgatt tctgcccgat ctcccgcgcg acgccatgcc
     2221 cgacctttat cgcgccgccg acgccttcgt cctgccgtcg ctgcacgaaa tgttcggcat
     2281 cgtgttgctg gaagccttgg cgacgggctt gccggtcttg tgcaacgaca cgcaggactt
     2341 ccgggccatt gctgggcctg gcggccggta cggcgacttt ggcgcccggg atggcctggc
     2401 ccgtggcctc accgacttgc tcgatccgat gttgcgagca accaccgccc gggcggcgcg
     2461 cgcccatgtc gagcggcatt tttccgaagc tgccgtaatg gggtcgatca ccgccatgta
     2521 ccgctccgtc cacgcggcgt cttcccatgt ccgatgacag gcaaggcacg gtcagcgtca
     2581 tcatcccagc gtacaacgcc gccgcctata tatcccgagc gatcgaaagc gcgctggccc
     2641 aaacccatgc gcccctggaa atcctggtca ttgacgatgg ttccagcgat gcgaccccgg
     2701 aaattgtggg gcgcctgcca gtcccggtgc gtctggtccg caagtcgaac ggcggccccg
     2761 cctcggcgcg caacctcggc gccagtctgg cgcgcggcga gtggctggcg ttcctcgatg
     2821 ccgacgactg gtggttcccg gaaaagctgc gggttcagct tgaatacgcc accggtcccg
     2881 aaatcgggat gctgcattgt ctgccggacc atcgggacga cgacgtgccg ccgctgctgt
     2941 cgttcgagca catgtgggac cgcaactgga tcattaattc gtccgtcctg atccggcgcg
     3001 tcgctttcaa cgcccttggc ggctttaacg agtcaagagc actcataagc gtcgaagact
     3061 acaatctgtg gatacgcgcc gccgcgtccg gctggaaagt cgttacttgt ccgcgcgtcc
     3121 tggtacatta cacccgcggc atcggcattt cttccaaccc gggacgcttc atgcaagcgt
     3181 cgctctacaa cgtggatgac atcggcgggc gcctgtcgct gccgctggcg acgatcaaac
     3241 gcaagcgcaa cgagatcatc ttcgacttcg gccgcaaggc gctgttcgag cgcgacctcg
     3301 gcacagcccg tcgattgttg cggcttgcat tcaaggaacg accaaacgcg cgcaatcttc
     3361 tgcacatggg cgtcgccgcg atgccggtgc ctgtgctgga cgcgcgacgt agcctgctgc
     3421 gatttctgga acccggcaat gccgcgcagg acgacgccag gccgtcggca tccaaaccag
     3481 aagggcagcc cgaaacggcg ccacggtttg catcgattct cgatcccgca cgtcacgcgg
     3541 cggcgcggga acagcggctg gatcggccta tgctgatcac gacgatcgac gccgaggaag
     3601 atttcgactg gacgaggcca ttttcccgag cggcgaccga tgtgaagtcg atacgctcgc
     3661 agcatcgcgc acatcgtgtt tttgagcggt atggcgtggt tccgatctat atggtcgatc
     3721 atcccgtcgc ctcccaggat gccggacggg cccctctgcg ggaactgttg cgatccggtg
     3781 cgtgcgacat cggggcgcag ttgcatccct gggtgacgcc acccttcgtt gaagatgttt
     3841 ccacccacaa cagctatgcg ggcaatcttc ctcggccgct cgagttcgca aagatcaagg
     3901 tgctgactca tgagctggca caagccttcg gtcagccacc gcgcatgttc cgagccggcc
     3961 gttacggggc aggccgcaac accggcggca tcctaagtca gttcggatat gaggcggaca
     4021 gcagcgtcgt gccgtgctgg agtttcgccg cgcagggcgg accggatttc cggcgcgtca
     4081 atgcaagccc gtactggatc gacgccgaac gacgcctcct ggaactaccg gtcaccgccg
     4141 cgatcgtcgg acgcgcggcc ggactgccgg atgcgctgac ctcgcggttc ttcgggcggt
     4201 taagtgaagc cacggcgctg ccctcgatca tgtcccgcct gggattgctg gaacgcatca
     4261 agttgacgcc cgaggggatc accatcgagg aagccaagcg gctggtccgg cacatggtgc
     4321 gtctgggaca caaggtattc gtgctgacct accacagccc ttcactggaa ccgggcaaca
     4381 cgccctatgt ccggagcgag gcggacctgg ccaagttctt gcgctggctc gacgaattct
     4441 acgattactt cacgcgcgaa atcggcggcg tctgtgcgac ctggcgcgag gtccgttcgg
     4501 cactggagca ggatcacgcc ggagcggttt ccgctcacgt tggctcgcag acatcgcctt
     4561 aagcccatcg tttcagggta tgattcacct ctccggtggt cccacctgca aggatcacgc
     4621 cctaagccgc aggtcgatca acgacggtcc tgaagcgcgg ctactccgcg gccaggggaa
     4681 ccaccgcgcc gccaggaact tcgtccaggt gacaggcgga aagatgcccc tcgccgactg
     4741 aacgcaacaa cggttcctcg gatcggcacc ggtcaaaagc cagcgggcag cgggtgtgga
     4801 agcggcatcc cgagggcggg ttgatcgggc tgggaacgtc gcccttcagg ataatccgct
     4861 gccgcttggc tgtcggatcc ggtaccggca cggcggacaa cagcgcccgc gtgtaagggt
     4921 ggtgcggttg agcgaacaac gttcgccggt ccgtcagttc gacgatcttc cccaggtaca
     4981 tcaccgccac gcgatgcgtc agatattcca ccgtcgccag gtcgtggctg atgaacagca
     5041 gtgccagccc cagttcatcc tgcaaatcgg ccagcagatt gatgatctgc gccttgaccg
     5101 acacgtccag cgcggagact gcctcatcgc agatgatcag gtccgcaccc ggcgccaaag
     5161 ccctggcaat gccgatccgc tgccgctggc cgccggagaa ctcatgcgga aaccgattga
     5221 tcgcatcgcg cggcaaacgc accttgtcca tcagcgcctc gaccttcgcg gtcaggtcgc
     5281 ggcggccatg cgccatgccg aagttgatca gcggttcggc caggatgtcg cgcaccttca
     5341 tccgtggatt gaggctgctg aacgggtcct ggaacaccac ctgcacccgc ttgcgcatca
     5401 tcctcagctt gccggccgcc atgttgtcga tgcgctcgcc gtccagatag acctcgcctt
     5461 cggtcagctt gaacagccgc aggatcgcct tgccgacggt ggatttgccg caaccggact
     5521 cccccactag cgacaacgtt tcgccgcgcc tgatatcgaa cg
//
LOCUS       DASWQN010000337         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1880902, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000337 DASWQN010000000
VERSION     DASWQN010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880902"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VGC82_05535"
     CDS             complement(<1..199)
                     /locus_tag="VGC82_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422768.1"
                     /translation="MAFDPVPAATALRQIRQDRVVVSPLADDIAPKTEAEGAAVQFAL
                     ARLTGAVPPFGFKIGATGKRMQ"
     gene            complement(199..984)
                     /locus_tag="VGC82_05540"
     CDS             complement(199..984)
                     /locus_tag="VGC82_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0422769.1"
                     /translation="MARPRFSAPAQRPRDGDGHRVTPDAPKGTVWLYGHHAVAAALVN
                     PNRRLRRLLLTDEAEAAISKHLPPPWALSPERVDRGRLDHLLGRDVVHQGAALLADPL
                     APPSLQSVLEKSGVILVLDQVTDPRNVGAIMRSAAAFGAAAVITQDRNAPEETGALAK
                     AASGALEKIPLLRAVNIARTIIALKAANVWCIGLDSTGKALSGPAFSGRRVALVLGAE
                     SEGLRRLTRETCDEIAGLTIAGAVESLNVSAAASVALYELARR"
     gene            complement(993..1301)
                     /locus_tag="VGC82_05545"
     CDS             complement(993..1301)
                     /locus_tag="VGC82_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422770.1"
                     /translation="MAAVPADRLALPPIAVLFEDRADRDPGRLDKVRGVTRAAAMPAI
                     ATVRSRATMVRAPRVSKRRTVGPDARTLILAPRAPGTSHARRVRIMRRGLRGKISSHL
                     "
     gene            complement(1324..2481)
                     /locus_tag="VGC82_05550"
     CDS             complement(1324..2481)
                     /locus_tag="VGC82_05550"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0422771.1"
                     /translation="MLTFFTHVGRMRRLETLDGLRGMLAVYVLLGHMAPFAVLPDRIQ
                     DAVSHGGAAVDLFFVLSGLVITQSLIRAGGETLPFLVARAARIFPVFLAVFAVAVIVQ
                     PWSCGFEHMPWISEANAARTICAEAWPAAWLAEIVAHLTMTHGLFPAALLPNVWVSFL
                     GAAWSLSTEWQFYLLALFAARRSRQLCWILLGLAAAGVAWHLTVAEPWQFSRAFLPNK
                     AHFFALGVASVPVVRRERGALRRYAAVLSACTVICFANGTFAKMLPPLVWTLCLMVQM
                     RPDTTGLRLAGLLLRSRTARYLGAISYCLYLVNEPIQKMVGSALSQLAGGDSVVFTTL
                     WIPLALGLPVLVSTWLHVYLEAPVLRWGRVAAARMASQRTPASVQAGGGGL"
     gene            2579..3118
                     /gene="rimP"
                     /locus_tag="VGC82_05555"
     CDS             2579..3118
                     /gene="rimP"
                     /locus_tag="VGC82_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY0422772.1"
                     /translation="MFGRPGLDGNEATSTTLEGKLAAIIQPRLELMGFDLVRVAVLGR
                     ERPTVQVMADRADGSQITVEDCQQISHDISAAIDVDDPIPGAWTLEVSSAGIDRPLTR
                     IKDWNRFAGHLARVETFFPQNGRKRFAGVVLGADEEGARLRLDGDVEVVIRHPDIRRA
                     KLVLTDALIEATAAKPLAN"
     gene            3139..>3653
                     /locus_tag="VGC82_05560"
     CDS             3139..>3653
                     /locus_tag="VGC82_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEY0422773.1"
                     /translation="MDTAIARPELLLVADAVAREKNIDREEVLEAMEQAIQKAGRAKY
                     GHEKDIRATIDRKSGDVRLSRWTEIVDTVENEETQLRKADAAKLFPDKKVGEFVVDPL
                     PPIDFGRIAAQTAKQVIVQRVREYERKRQYEEFKDRVGEIINGVVKRTEYGNLMVELG
                     RAEALLRRDEL"
BASE COUNT          725 a         1203 c         1167 g          558 t
ORIGIN      
        1 cctgcatccg tttgcccgtg gccccgatct tgaatccgaa tggcggcact gcgccggtca
       61 gcctggcaag cgcgaactgc acggcagcgc cctcggcctc ggtcttcggc gcgatatcgt
      121 ccgcaagcgg agacaccacg acccgatcct gacggatttg gcgaagtgcg gtcgctgccg
      181 gaacgggatc gaaagccatc agcgccttgc aagctcatac agcgcgacgg aggcggcggc
      241 ggaaacgttc aagctttcta cggcgccggc gatggtcagc ccggcgatct cgtcgcaggt
      301 ttcgcgcgtg agacgccgca agccctcact ctcggcaccc agcaccaaag ccacgcggcg
      361 accggagaag gccggaccgg acaacgcttt gccggtgcta tccaggccga tgcaccagac
      421 attggcggct ttcagggcga taatcgtcct ggcgatgttg accgcccgca gcaacgggat
      481 cttttcgagc gctccggacg cggccttggc gagagcaccg gtttcctccg gcgcgttgcg
      541 atcctgggtg atgaccgcgg cagcaccgaa cgctgcggcc gaccgcatga tcgcacccac
      601 gttgcgcgga tcggtcacct gatccagcac caggatgacc ccggactttt ccagcaccga
      661 ctgcaggctg ggcggcgcca gtgggtcggc cagcagtgcc gcgccctggt gcacgacatc
      721 gcgtcccaac aggtgatcga gccgtccgcg atcgacccgt tcgggagaaa gggcccacgg
      781 cggcggcagg tgcttgctga tcgccgcctc ggcttcgtcc gtcagcagca gccggcgcaa
      841 ccgacgattt ggattgacca gagcggcggc tactgcgtgg tggccataca accagacggt
      901 tcccttcggc gcgtccggag tgacacggtg gccatcgccg tcacgcgggc gctgcgccgg
      961 agcggaaaag cgcggccgcg ccacatcctg ctttacaggt ggcttgaaat cttcccgcgc
     1021 aacccgcggc gcatgatcct cacgcggcgc gcgtggctgg ttcccggtgc tcggggtgcg
     1081 aggattaaag tccgcgcgtc cggccccacc gtgcggcgct tgcttacccg gggggcgcgg
     1141 accatcgtgg cgcggcttct gaccgtcgcg atagccggca tcgccgccgc gcgcgtcacc
     1201 ccgcgcacct tgtccaggcg gccggggtcc ctgtccgctc ggtcctcgaa aaggaccgcg
     1261 atcggaggga gggcgagacg atccgccggg acggctgcca tgaggaggtt tcattccccg
     1321 ttcttatagc cctccgccgc ctgcctgcac agatgccggc gtccgctgcg atgccattcg
     1381 tgccgccgcg actcgtcccc aacgcagcac gggggcctcg agatagacat gcagccatgt
     1441 cgagacaaga accggcaaac cgagcgccag cgggatccac aatgttgtaa acacaacgct
     1501 gtcgccgccg gcaagctggc ttagcgctga accaaccatc ttctggatgg gctcgttcac
     1561 caagtaaagg cagtacgaaa tcgcgccaag atagcgcgct gtccggcttc gcagcagtag
     1621 tcccgccaag cgaagcccgg tggtatccgg ccgcatctgc accatgagac agagcgtcca
     1681 gaccagcggc ggcagcatct tggcgaacgt gccattggca aagcaaatca ccgtgcaggc
     1741 ggacagcacg gcagcgtagc ggcgtaacgc cccgcgttcc cggcgaacga ccggaacgct
     1801 ggccacgccc agggcgaaaa aatgcgcctt gttcggcaga aacgcccggc tgaactgcca
     1861 gggttcggca acagtgaggt gccaggcaac ccccgccgcg gccaacccaa gcaggatcca
     1921 gcataactgg cggctgcgac gggccgcgaa aagcgccagc agatagaact gccactcggt
     1981 cgaaaggctc caggcggcgc cgaggaagct gacccagaca ttcgggagaa gtgctgcggg
     2041 aaacaacccg tgcgtcatcg tcagatgtgc cacgatctcc gccagccaag cggccggcca
     2101 tgcctcggcg cagatcgttc tggcggcgtt ggcctcactg atccacggca tgtgctcaaa
     2161 gccgcaggac catggctgca caatgaccgc taccgcgaac accgccagga ataccggaaa
     2221 aatccgcgcc gcgcgggcga ccaggaacgg caaggtctcg ccgccggctc gtatcagcga
     2281 ttgagtgatg accagtccgc tgagcacgaa aaacaagtcc accgccgcgc cgccatgcga
     2341 caccgcgtct tgtatccggt ccggcagcac ggcgaacgga gccatgtgcc ccagcaatac
     2401 ataaaccgcc agcatgccgc gcaggccatc aagggtttcc agccgcctca tgcggccaac
     2461 atgcgtgaaa aaggttaaca aggtacgaag ccttgcaatc agccgttgcg cgcgctattt
     2521 cacagtgaaa tcattctccg ctgagatacg gggggtggcc ctcgcaggcc gccttttttt
     2581 gtttggacga cctggtttgg acggaaacga agcaacatcc acgaccctgg aaggcaagct
     2641 ggctgcgatc atacagccac gcctggaact catggggttc gacctcgtcc gcgtcgccgt
     2701 tcttggccgc gaacgcccga ccgtgcaggt catggccgac cgcgccgacg gctcgcagat
     2761 caccgtcgag gattgtcagc agatcagcca tgacatcagc gcagcgatcg acgtcgatga
     2821 tccaattccc ggcgcctgga cgctggaggt cagctccgcg ggaattgacc gcccgctgac
     2881 ccggatcaag gattggaacc gatttgccgg ccatcttgca cgggttgaaa cgttttttcc
     2941 gcagaacggc cgcaagcgat ttgccggcgt cgtgctgggg gccgacgagg agggtgcccg
     3001 ccttcgcctc gacggcgatg tggaggtggt gatccgccac cccgatattc gccgcgcgaa
     3061 actggtcttg actgacgcgc tgatcgaagc cacggcggcc aagccgttgg ccaactgaat
     3121 gtactgacgg aggtcgccat ggacaccgct attgcccgcc ctgaactgct gctggtcgcc
     3181 gatgccgtcg cacgcgaaaa gaacatcgac cgcgaggaag tgctggaggc gatggagcag
     3241 gccatccaga aggccggccg ggcaaaatac gggcacgaga aggacatcag ggcaacgatc
     3301 gatcgcaaaa gcggcgacgt gcgcctgtct cgctggaccg agatcgtcga tacggtcgaa
     3361 aacgaagaaa cccaactgcg caaagccgac gcggccaagc tgttcccgga caagaaggtc
     3421 ggggagttcg tcgtcgatcc gctgccgccg atcgatttcg gccgcatcgc cgcccagacc
     3481 gcgaaacagg tcatcgtcca gcgggtccgc gaatacgagc gcaagcggca gtacgaggag
     3541 ttcaaggacc gcgtcggcga gatcatcaac ggcgtggtga agcgcaccga gtacggcaac
     3601 ctgatggtgg aactcggccg cgccgaggca ctgctgcggc gcgacgagct gat
//
LOCUS       DASWQN010000338         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1616762, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000338 DASWQN010000000
VERSION     DASWQN010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616762"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VGC82_05565"
     CDS             <1..771
                     /locus_tag="VGC82_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422774.1"
                     /translation="VDPAIPSLPPEMGRGYILPFPILPMTRFRSIFQPTPMSTVQTNL
                     PIRLDVFDTEGTKQAEKFLGCLERAHDLAMDLDDLGVDQGHGELVYDFRNGGEADGWL
                     HALFRYEDRQSGHAAESSFGAHVFNTAMTYRDEPQSYSGPPPGLSTRLFLRLGDRTRR
                     GFAMLIYTASGPWHPRSSTVLELHDGTGASVARTSVSIACSGSLMIWPHTMFDASILA
                     AAGPEAYVLVRDSTCRLFGYHGLMDDAGGFSLDHMFGF"
     gene            1020..1190
                     /locus_tag="VGC82_05570"
     CDS             1020..1190
                     /locus_tag="VGC82_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422775.1"
                     /translation="MSHSTPFAVEEHVHGETVWVAVRSRGADWSWLTPDEAVQLGRSW
                     IEKYSPLAEAAD"
     gene            complement(1230..2096)
                     /locus_tag="VGC82_05575"
     CDS             complement(1230..2096)
                     /locus_tag="VGC82_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0422776.1"
                     /translation="MSDIALRPEIVQAPRGAQDFGIVEKPLTAFERMYNQAWARKTFL
                     IVVLVIAWELYARHLDNPLLVPTFTDTITAWWDGIRSGVLPDRALSSLRVLAIGFSTG
                     VVLAFILTVLAITTRIGTDFLESMTAMFNPLPAIALLPLALIWFGLGIGSLVFVLVHS
                     VLWAVALNTHAGFRGVSQTLRMVGRNYGLSGLPLVLKILIPAAFPSILAGLKIGWAFA
                     WRTLIAAELVFGVASGKGGLGWYIFESKNQLDIPEVFAGLLTIIMIGLMVENVVFRTI
                     ENRTVRRWGMQN"
     gene            complement(2089..>2781)
                     /locus_tag="VGC82_05580"
     CDS             complement(2089..>2781)
                     /locus_tag="VGC82_05580"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0422777.1"
                     /translation="VSFDVHRADRFVVLGPSGCGKSTLLKAVGGYMAPTEGSMRLKGA
                     AIRGPGADRVFVFQEFDQLLPWKTVRQNVTFALEASRKLRGEAAQAQAARYIEKVGLA
                     KFADSYPHTLSGGMKQRVAIARGMAMEPDILLMDEPFAALDALTRQRMQEELLMLWDE
                     TRFTVLFVTHSIPEAVKIGNRILLLSPHPGQVKAELNSVPPNGDPAVAAALEARIHDM
                     LFADRVEEEEHV"
BASE COUNT          536 a          909 c          851 g          485 t
ORIGIN      
        1 gtcgatccag ccatcccttc gctgccgccg gaaatgggac gtggctacat cctgcccttc
       61 ccgatcctgc cgatgacgcg ctttcgttcg atattccagc cgacacccat gtccacggtg
      121 cagacaaacc tgccgattcg actggatgtg ttcgataccg aggggacgaa gcaggcggag
      181 aagttcctcg gctgcctgga acgagcccac gacctggcaa tggatctgga cgatctcggc
      241 gtggatcaag gccacggcga actggtctat gattttcgga acggcggcga ggccgacggc
      301 tggctgcacg cgcttttccg gtacgaggac cgccagtccg gccatgccgc cgagtccagt
      361 ttcggcgccc atgtcttcaa caccgcgatg acctatcgcg atgagcctca atcctactcc
      421 ggccctcccc ccgggctatc gacgcggctg ttcctaaggc tcggggatcg cacgcgacgg
      481 ggtttcgcga tgctgatcta tacggcgtcc ggaccgtggc atccccggtc gtccaccgtc
      541 ctggaattgc atgacggcac cggggcgtcg gttgcaagaa cctcagtatc catcgcctgc
      601 tcgggatcgc ttatgatctg gccgcatacg atgttcgatg cttctatttt ggctgcggcg
      661 ggaccggaag cctacgtcct ggtgcgtgat tcgacgtgcc ggctgtttgg atatcatggc
      721 ttgatggatg atgcgggcgg cttctcgctg gaccatatgt tcggcttcta agctgccacg
      781 gaaggctgcc gcccctcctg ttgccgggat cacacattcg caatgaccta agcgactatc
      841 ttcgccgccc ggtcaacaac caggattttc ctcttgcggc acggcgggcg aatacggcaa
      901 gagtgcacct gtgccgatca caatgtcgcg agacaccaaa acgtgcacga agatccccgc
      961 aagcgttatg tggggtagcg caaccatact cactgcaatt catattcacg aggcacacga
     1021 tgtcgcactc cacgcccttc gcggtcgaag agcacgtgca cggggaaacg gtttgggttg
     1081 ccgtccgctc ccgcggcgcg gactggtcct ggctgacgcc agacgaagct gttcagctcg
     1141 gccggtcctg gatcgagaag tacagcccgc tggcggaagc cgcggactga ccgccggtca
     1201 atgaacaggc agcagggctt gaagacactc tagttctgca tgccccagcg ccggacggta
     1261 cggttctcga tggtgcggaa caccacgttc tccaccatca ggccgatcat gataatggtc
     1321 agcaggccgg caaagacctc cggaatatca agctggttct tgctttcgaa gatataccag
     1381 cccaaaccac cctttcccga cgcgacgccg aacaccagtt cggccgcgat cagcgtgcgc
     1441 caggcaaagg cccagccgat cttcaggccg gccaggatcg agggaaacgc cgccggaatc
     1501 aggatcttca gcaccagggg caggcctgaa aggccataat tccgcccgac catgcgcagt
     1561 gtctgcgaca cgccgcgaaa ccccgcatgc gtattcaggg cgactgccca cagcaccgaa
     1621 tggaccagca cgaagaccag gctgccgatg cccaggccga accagatcag cgccagcggc
     1681 agcaacgcga tcgccggcag cgggttgaac atcgcggtca tggattccag gaagtcggtg
     1741 ccgatccggg tggtgatcgc cagcaccgtc aggatgaacg ccagcacgac tccggtggaa
     1801 aagccgatgg ccagtacccg caacgaagac aaggcgcgat cgggcagcac accggaccga
     1861 atgccgtccc accatgccgt gatggtgtcg gtgaaggtcg gcaccagcaa cgggttgtcc
     1921 agatgcctgg cgtaaagctc ccacgcgatc accaacacca cgatcaggaa ggtcttcctc
     1981 gcccaggcct gattgtacat ccgctcgaag gccgtcagcg gcttttcgac gatcccgaaa
     2041 tcctgcgccc cacgaggagc ctggacgatc tccgggcgca gggcgatgtc agacatgctc
     2101 ttcctcctcc acccgatcgg cgaacagcat gtcgtggatg cgggcttcca gcgccgcggc
     2161 aacggcggga tcaccgttcg gcgggacgct gttcagttcg gctttcacct gaccgggatg
     2221 cggcgatagc agcaggatgc ggttgccgat cttgaccgcc tcggggatcg agtgggtcac
     2281 gaacagcacg gtgaaccggg tctcgtccca cagcatcagc agttcctcct gcatccgctg
     2341 gcgggtcagc gcgtcgagcg cggcgaacgg ttcgtccatc agcaggatgt cgggctccat
     2401 cgccatgccg cgggcgatcg cgacgcgctg cttcatgccg cccgacagcg tgtgcggata
     2461 ggaatcggcg aacttggcga gcccgacctt ctcgatgtag cgcgccgcct gcgcctgcgc
     2521 ggcctcaccg cgcagcttgc gcgaggcctc gagcgcgaag gtcacgttct gccgcaccgt
     2581 cttccagggc agcagctggt cgaactcctg gaacacgaac acgcggtcgg cgccgggccc
     2641 gcggatcgcc gcgcccttga ggcgcatgct gccctcggtg ggcgccatgt agccgccaac
     2701 ggccttcagc agggtggact tgccgcagcc ggaaggtccc agcaccacga aacgatcagc
     2761 ccggtggaca tcgaagctga c
//
LOCUS       DASWQN010000339         2402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_561151, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000339 DASWQN010000000
VERSION     DASWQN010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561151"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VGC82_05585"
     CDS             <1..315
                     /locus_tag="VGC82_05585"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY0422778.1"
                     /translation="LYGLNNSNPFNPLFLPQGTGIGFVPGGIITFGGGLALYSGGKVI
                     GGLGVSGDTSCADHAIAYRMRRLAGLDGTPNSDNIVYLGPSDEPNVGFKQPHCTKDDI
                     TP"
     gene            299..922
                     /locus_tag="VGC82_05590"
     CDS             299..922
                     /locus_tag="VGC82_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0422779.1"
                     /translation="MTSRLKPRRSEAPVALRGFTLLEVLVALAVVGLFLLGLVQGSRF
                     VLFGWDRQVRVVARIEELDAVDRTLRRLVALARPGSEWERLVFVGGLHSVAFTSVLPL
                     PAVGSPTQRVDVELVVDADHRLLLKWTPHLHVIRTGPAPSLVSTEILQAVERLELSYW
                     PAAHHGGWTTNWHDPVPPLLVRIRIVFPEASNLWWPDIVVAPMLEPP"
     gene            995..1789
                     /gene="hpnK"
                     /locus_tag="VGC82_05595"
     CDS             995..1789
                     /gene="hpnK"
                     /locus_tag="VGC82_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HEY0422780.1"
                     /translation="MKTVTFSADDFGLTEAVNEGIERAHRDGVLQAASLMVAAPAAAD
                     AIRIARANPSLRVGLHLVVIEGPAVLPPSAIPDLVDAAGQFPSDQLKLGFYYFFRSRV
                     RRQLRAEISAQFAAFAATGLTLDHANAHKHMHLHPTVGAMMLAIGRNFDLHRIRVPAE
                     PPVVMAHCGSRIGWGGRLLYHWTALLRYQARTAGVSSNDHCFGLEWSGRMTTDRVRCL
                     LANLPDGDSEIYFHPATRRDALLDRLMPHYEHEAELASLLDKADGV"
BASE COUNT          419 a          714 c          750 g          519 t
ORIGIN      
        1 ctttacggcc tgaacaactc taacccgttt aacccgctgt tcctgccaca aggcaccggc
       61 atcggcttcg tgccgggcgg catcatcacg ttcggcggtg gtcttgcgct ttattccggc
      121 ggaaaggtga tcggcggact cggcgtcagc ggcgatacgt catgtgccga ccacgcgatt
      181 gcgtaccgga tgcgccgcct cgcgggattg gatggcacgc cgaactccga caatatcgtt
      241 tacttaggcc catccgatga gcccaatgtc ggcttcaagc agcctcattg cacgaaggat
      301 gacatcacgc cttaagccac ggaggtcgga agccccggtc gctcttaggg gcttcacttt
      361 gctggaggtg ctcgttgctc ttgcagtagt gggcttgttt ctgcttggtc ttgtgcaggg
      421 tagccggttc gtgctgttcg ggtgggaccg ccaggtccgg gtggtggcga ggattgaaga
      481 gctcgatgcc gtggaccgga cgctgcggcg tctggtcgca ctcgcccggc cgggttcgga
      541 atgggagcgc ctggtgttcg tcggcggtct ccattcggtc gcgtttacca gcgtcctgcc
      601 gctgccggcg gtcggatccc ccactcagcg tgtcgatgtg gaactggtgg tcgatgcgga
      661 tcaccgcttg ctgctgaaat ggacgccgca tctgcacgtg attcggactg gacccgctcc
      721 gtcgctggta tcgactgaaa tcctccaggc ggtcgagcgg ctcgaactgt cctattggcc
      781 ggcggcacat cacggcggtt ggacgacgaa ctggcacgat ccagtgccgc cgcttctggt
      841 ccgaattcgg atcgtgttcc cggaggccag caatctgtgg tggcctgata tcgtggttgc
      901 accgatgctg gagccgccat gaccctgtgc tttccccggg tgcgatcatg ttctacggtt
      961 ccgtgatgcc acccgtcgcg ctccgcgcca tcgcatgaag accgtcacct tctccgccga
     1021 tgattttggc ctgaccgagg cggtcaacga gggcatcgaa cgcgcgcacc gggatggtgt
     1081 cctgcaggcg gcgagtctca tggtcgccgc ccccgcggcc gctgacgcga tacggatcgc
     1141 acgagcgaac ccatcgctgc gggtcggcct gcacctcgtg gtcatcgagg gacccgcggt
     1201 gctgccgccg tctgccattc cggaccttgt tgacgcagcc gggcaatttc cctccgacca
     1261 attgaagctt ggattttact acttcttccg cagccgtgtc cgccgccagt tgcgggccga
     1321 aatcagcgcc cagtttgctg cgttcgcggc caccgggctg acattggatc atgccaatgc
     1381 tcacaagcat atgcacctgc atccaacggt tggggccatg atgttggcca tcgggcggaa
     1441 tttcgacctg caccgcatcc gggttccggc tgaaccgcca gtggtcatgg cgcattgcgg
     1501 gagccggatt ggatggggcg gcaggctgct ttatcattgg acggcgctgc tccgttatca
     1561 ggcccgcact gccggcgttt ccagtaacga ccactgtttc ggactggaat ggagcggccg
     1621 tatgacaact gaccgcgttc gttgtcttct tgccaatctt cctgatggtg acagcgaaat
     1681 atactttcat cctgccaccc ggcgagatgc cttgctggat cggctgatgc cacactatga
     1741 gcatgaggcg gaactggcat cattgttgga taaagctgat ggagtatagt gtgctctttc
     1801 gatagtgaat tattcttaat gcgagcagaa cgaggccacc aaggggcgtg atcacgctgg
     1861 cagaacgtcc aaagcgtgtg aatcgcgtaa taacaaggag ataggccatt tgcggccggc
     1921 acagtcaggc tgaatatggt ctagatctcc aggacgaaag cgcattggca ccggaacacc
     1981 aacgggggtc cactgcatgg acatcccata aaacccctga ctgttacccg gtaacgcatt
     2041 ctgttggctc ttcatgtaga acgatgtcct gcgccgcaat ccgcacagat ggtccgatcg
     2101 tcggcaatgt gaactgtctt gccgttcatg cttgcgcttc cacgatggtg ggggtactcg
     2161 ctgcttgtcg ttcctcgccg ggtgcggcaa catccgggca ggtcagccgc tgccgcatga
     2221 gggagacatg gcaaagacct ccgcgccgaa aaagcctgcc gcgacgggca tgttcagcct
     2281 gaaagtgacg ctgcgcggca tcaagccgcc gatctggcgg cggatcgtgg tgccaggccg
     2341 catgacgctg ggcgatctgc atgtcgcgat ccagatcacc atgggttggg gggacggcca
     2401 tc
//
LOCUS       DASWQN010000340         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1881057, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000340 DASWQN010000000
VERSION     DASWQN010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881057"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VGC82_05600"
     CDS             complement(<1..347)
                     /locus_tag="VGC82_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422781.1"
                     /translation="MAITLEKFAADCHAALKTQPGTPGREKVRALVQQVLADPEFVAT
                     YIPPGTPERHLLYEDAELGFAILAHGYVGPKGSKPHDHGPSWAIYGQASGETVMTAWD
                     CVARPTDSAPGKAV"
     gene            complement(413..745)
                     /locus_tag="VGC82_05605"
     CDS             complement(413..745)
                     /locus_tag="VGC82_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422782.1"
                     /translation="MRHAVEKHYRRERERAADAFHPTTSVFIEQDINKEAHIRRAMPQ
                     FLTLRDQDGKALATAMLPPKGRTSNGCIIVGPANADPYPEHGDAIAALAAHFGVTLDR
                     SSCYPYRR"
     gene            1012..2655
                     /locus_tag="VGC82_05610"
     CDS             1012..2655
                     /locus_tag="VGC82_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0422783.1"
                     /translation="MADTFDYVIVGSGAAGSVLCARLTEDPSITVCVLECGPPDRHPY
                     IHIPAGFIKMLFNPAYTWQFQTEPGEGINGRRIPTTQGRTLGGSSSINGMIYNRGQRE
                     DFDHWAQRGNRGWGYVDILPYFKRGERRLGAGDERIHGRAGSLPVTDNDWDHPAMEAF
                     IRGAEELGMPRCVDYNSGDDQMGVGYFQRLIHRGYRRSAARAFLYPARATGRLEVRTN
                     ARAAQILFDGTRATGVRYANDRDPSVRQEVFVRREVIVSSGTANTAKLLQMSGVGPAW
                     LLHDLGVPVIADLPVGENFRDHYSTRIVARVKNIRTINEISRGLGLMGQVARWATGRP
                     SILAVSPSILHWFWKTEDTMRQPDIQGVFSPASYRQGFVGLLDDYPGMTCGVWQHRPE
                     STGYVRARGTDPFVDPVIQPNYLKDSMDQRVLVKAMHWARKLLHTNALAPYFDVDTLP
                     GPDVHTDDEWLDYARQYGSTAYHLIGTARMGPATDKTSVVDDQLRIHGLQGIRVVDAS
                     IMPSMPSANTYSSTMMIAEKASDMIRGRTPLPPVVGIAA"
BASE COUNT          596 a         1042 c         1101 g          648 t
ORIGIN      
        1 accgctttgc ctggcgcgct atcggtcgga cgggcgacgc agtcccaggc agtcatgacc
       61 gtctctccgc ttgcctggcc gtagatcgcc caggacggac catgatcatg cggcttgctg
      121 cccttcggcc cgacgtagcc gtgcgccagg atcgcaaaac cgagttccgc atcttcgtac
      181 agcagatgcc gttccggtgt tccgggcggt atataagttg caacgaactc cgggtccgcc
      241 agaacctgct gcaccagggc gcgaaccttt tcgcgtccgg gtgtgccggg ttgggtcttt
      301 aaggcggcat gacagtcagc ggcgaacttt tccaacgtga tagccatggc attgctcctg
      361 cagaaggcct tgtcgattac gtcatttcac ggcgtttgcc ggtcatcatg tcctatcgcc
      421 gataaggata acaactgctg cgatcgagcg tgacaccgaa gtgggccgcc agcgcggcga
      481 tcgcgtcgcc atgttccggg taagggtcgg cattggccgg gccaacaatg atgcagccgt
      541 tgctcgtcct tcccttgggc ggcagcatag cagtggccag cgctttcccg tcctggtcgc
      601 gcaaggtcag gaattgcggc atcgcccgcc ggatatgggc ttccttgttg atgtcctgct
      661 cgatgaacac agacgtggtc ggatgaaatg catcggcggc gcgttctcgc tcgcgccggt
      721 agtgcttttc gaccgcatgc cgcatctcca gcgactcggc tacggcttcg ccttccgtga
      781 caaggcggaa ccaggtccga tcatgcggtg cgtcgcagac atattccatg ggcgatcccc
      841 ctgatttgga tggcctgttg gcttggacgg cctgggcggc ctgagttgct cggacggtct
      901 gagtggcttg gacggcctgg gtagcttgga ttgatttgaa acggcagcat aggatgaaat
      961 tcgcctgcga tcaaacggcc ggcgagccgg gttgggatta tggggttaag aatggccgat
     1021 acgttcgact atgtgatcgt aggcagtggt gccgccggct ctgttttgtg cgctcggctg
     1081 accgaggacc caagcatcac cgtctgcgtg ctggagtgcg ggccgccgga ccggcatccg
     1141 tacatccaca tcccggccgg cttcatcaag atgctgttca acccggccta tacctggcag
     1201 ttccagaccg aaccgggcga aggcatcaac ggccgccgca tccccaccac ccaggggcgc
     1261 actctgggag gatcgagttc catcaacggc atgatctaca accggggtca gcgcgaagac
     1321 ttcgaccact gggcgcaacg cggcaatcgc ggctggggct atgtcgatat cctgccctac
     1381 ttcaagcgcg gtgaacgacg gctcggcgcc ggcgacgaac gcatccacgg gcgcgcgggg
     1441 agcctgccgg tcaccgacaa cgactgggac cacccggcga tggaggcctt catccgcggt
     1501 gccgaggaac ttgggatgcc gcgctgcgtg gactacaaca gcggcgacga ccagatgggc
     1561 gtcggctact tccaacggct gatccatcgt ggctaccggc gttcggctgc tcgggcgttt
     1621 ctgtatccgg cgcgcgcgac tggccggctg gaggtgcgga cgaacgcgcg agctgctcag
     1681 atcctgtttg acggcacgcg ggcaaccggc gtccgctatg ccaacgaccg tgatccttcg
     1741 gtgcggcaag aggtgttcgt tcggagggag gtgatcgttt catccggcac cgccaatacc
     1801 gcgaagctgt tgcagatgtc cggtgtcgga ccggcgtggt tgctgcacga cctcggcgtg
     1861 cccgtgatcg ccgatcttcc ggtcggggaa aactttcgcg atcattattc cacccgcatc
     1921 gtggcgcgcg tgaagaacat ccgcacgatc aacgagatat cgcgcggtct tggcctgatg
     1981 ggacaggtcg cgcggtgggc gacgggccgg ccgagcatcc tggcggtgtc gccgtccatc
     2041 ctgcattggt tctggaagac cgaggacaca atgcgccagc ccgatatcca gggcgtgttc
     2101 agtccggcca gctacaggca agggtttgtc gggctgctcg acgattatcc ggggatgacc
     2161 tgcggggtat ggcagcaccg gccggagagc accggctacg tccgcgcccg cggtaccgat
     2221 ccgtttgtcg atccggtcat ccagccgaac tatctgaagg attcgatgga tcagcgcgtg
     2281 ctggtcaagg cgatgcactg ggcgcggaaa ctgctgcaca cgaacgctct ggcgccatac
     2341 tttgacgtcg atacgctgcc gggtcccgat gtgcataccg acgacgagtg gctggactac
     2401 gcccgtcaat acggatccac cgcgtatcac ctgatcggca ccgcgcgcat gggaccggca
     2461 acggacaaga cttcggtggt ggacgaccag ttgcgcatcc atggcttgca ggggatccgg
     2521 gttgtcgatg cctcgataat gccgagcatg ccgtcggcaa atacctatag ctcgacgatg
     2581 atgatcgccg agaaggcttc cgacatgatc cggggccgga cgccgttgcc accggtggtg
     2641 ggcattgctg cgtgatcgtg gttagcgttc acttccagtg aaaaccaccg gccgggacgg
     2701 cgcccctccg tccgctgcta ctcagccgcc agccgttgac ctggtggttg gacgacctgg
     2761 aagaatccgg ggcggcaggc gctgccacgg ccaagcctgt acgatgggcg aggcgctgca
     2821 atgtgtgcac gcgcggactc cggcattgca cgggcagccg ccatatgcgt ccgccgcagt
     2881 gcgcgccgtc ctgaccgctg aatcgacctt cgaccttggt ggcggccatc gtccgcgccg
     2941 tggggcgccg cgagcggacc cagccgagcc atgcggcggc cggctcgcct gaaccggaga
     3001 ccggtgctcg gctgttcgcc cttcacgata cggaaaccgg aacgaaccag cgctcgtaac
     3061 cttcgccaag gtcacacccg gtatcggtct gccggacccg gagcgtgctg tcgatcaggt
     3121 ccgtgcaggc gcctggtggc ggaatgatgc cgtgccagcc ctgcagcccg atccgccaga
     3181 gcggtttcac ggtggttgga aacagagcct cctgtggcta cgagaaagtc gtgggtgacc
     3241 gagtcaagca cggccatgac acatcgaccg caggcggcaa actggctcat aactttgcca
     3301 gtcagcttaa aaggatttta gccgcgcaat tacaggccgg tctgcggcac ggatttcatc
     3361 caatggttgg tcggtccact gtttcat
//
LOCUS       DASWQN010000341         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_825575, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000341 DASWQN010000000
VERSION     DASWQN010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825575"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(441..1646)
                     /locus_tag="VGC82_05615"
     CDS             complement(441..1646)
                     /locus_tag="VGC82_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010994077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEY0422784.1"
                     /translation="MGDVSAIGGRYTAVRRSLNERGRRLFAAAEARAAGHGGIVAASR
                     ATGIARSTIGRGLRDLDDPGALSGAVRRPGSGRHALTATDPTLLEDLHRLLEPATMGD
                     PMRPLLWVSKSHAKLAVALRAMGHRIGKSSIPKLLGLLKYRRQANRKTLEGSRNPDRD
                     AQFEHINAAVIANQTAGQPVISVDTKKKELIGPYKNAGSDYRPEGCPDLVKVHDFIDD
                     ELGRVVPYGVYDIAANAGCVSVGIDHDTASFAVNSIRRWLDVMGRERYPAANTLMITA
                     DGGGSNGSRVRLFKVELQKLADETGLVVQVCHFPPGTSKWNKVEHRLFCHITENWRGK
                     PLTSRMTVVELIAATTTTTGLTVRCELDPRSYPKGVKITDQEMASLNITGDAFHPEWN
                     YSIAPRCPL"
BASE COUNT          364 a          640 c          598 g          361 t
ORIGIN      
        1 aacgacacca ctgtgctcga gccggggatg gtcatcacga tcgagcccgg agcagcttat
       61 gatatgggcg gcgcacggaa ggtgatggtg cacgaggaga atgtggtcat cacggaggcc
      121 gagccgctgc tcctgtccgt ccgcgccccc aagcggatgc cggaattatc ccgctgatag
      181 gcctgaagcc tgcgtggcta aatcggtgcc gggagcgccc cctgccagac cgttggcctc
      241 atccagaagc ttcatccact tccgccgtat cagaaaaacg agcggtttcc ttgtggccgc
      301 ccgaggtggc cgaaaatacc tcgcaaagcg ccaggaaacc cgttgaggtt atgatcacaa
      361 ggttgcggcc tggccccggc ctatcttggt gatgcgggtt tgttgttgat cggctaaggc
      421 gacgcccaac aatgatcgca tcagagcgga catctcgggg cgatgctata attccattcg
      481 ggatggaatg cgtcgccggt gatgttgagg ctggccatct cctggtcggt gatcttgacc
      541 cccttgggat aggatcgcgg atcgagttcg catcgcaccg tcaggccggt tgtggtcgtg
      601 gtggccgcga tcaactccac gacggtcatc cggctggtga gcggctttcc gcgccagttc
      661 tcggtgatgt ggcagaacag ccggtgctcc accttgttcc atttcgatgt acccggcgga
      721 aagtggcaga cctgcaccac gaggccggtc tcgtcagcca gcttctgcag ctcgaccttg
      781 aacagccgca cgcgtgaccc gttcgagcca ccgccgtctg ccgtgatcat cagcgtgttg
      841 gccgccggat accgctcccg ccccatcaca tcgagccagc ggcggatcga gttgaccgcg
      901 aacgacgcag tgtcatggtc aatcccgacg ctgacacacc ccgcattggc cgcaatgtca
      961 tagacgccat agggcaccac acggccaagc tcgtcgtcga tgaagtcgtg caccttgacc
     1021 agatcggggc acccttccgg ccggtaatcg ctgccggcgt tcttgtaggg gccgatcaac
     1081 tccttcttct tggtgtcgac cgagatcacc ggctgaccgg ccgtctggtt ggcaatgaca
     1141 gccgcgttga tgtgctcgaa ctgcgcgtcg cggtcgggat tgcggctgcc ctcgagcgtc
     1201 ttgcggttcg cctgccgacg atatttcagc aggccgagca gcttggggat gctgctcttg
     1261 ccgatccgat gtcccatcgc ccgcaacgcc acagccagct tggcatggct cttggacacc
     1321 cacagcaacg gacgcatcgg gtcgcccatc gtcgccggtt ccagcaaccg gtgtaggtcc
     1381 tcaagcagcg tgggatcggt cgctgtcaac gcatgccggc cactgccggg ccggcggaca
     1441 gcaccggaca gtgcgccagg atcgtccagg tcccgcaggc cgcggccgat cgtgctgcgg
     1501 gcgatccccg tggcacggga tgcggcaacg atcccgccat gccccgccgc ccgcgcctcc
     1561 gctgcagcga acagccgccg cccccgttcg ttcaggctac ggcgaaccgc cgtataccgg
     1621 ccgccaatcg ctgatacatc gcccatcgcc ggagtctagg ccgcgacgaa acgcaccgga
     1681 atcctacatc tgacaccgca acaaccaata cctcatccga ttcgcacatt gttgggcgtc
     1741 gccttagtgc cggctgccac gacgccgata cgacagggag ggaaagcgcc gtcgtcgtaa
     1801 ggactgctta acatcacgga aacgtgagca caactggttt gcgagctcct gcccgaataa
     1861 gttgcaatgt cggctcagct ggatatctcc ggcaagaaca gcgcggcgac ggctcgctga
     1921 aaccagccca gatatcacgg ctctacgcac ggcgctggga agg
//
LOCUS       DASWQN010000342         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1617140, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000342 DASWQN010000000
VERSION     DASWQN010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617140"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1079
                     /gene="cyoA"
                     /locus_tag="VGC82_05620"
     CDS             219..1079
                     /gene="cyoA"
                     /locus_tag="VGC82_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HEY0422785.1"
                     /translation="MALLVQLTGCSDGVLNPQGPIGAANKLILLNSLAIMLAIVVPTI
                     LAALVFAWWFRASNTRAVYRPDWAYSGRIELIVWSIPIMIILFLGGITWVGSHDLDPA
                     QPIASQSKPVEVQVVSLDWKWLFIYPDLHIASVNQLVVPAAVPVHFALTSASVMNSFF
                     VPQLGSMIYTMNGMVTQLHLLADHEGDFFGESAMFSGDGFSDMHFIVRAVSVDDFTKW
                     TSTVQQTGPALDPSEYSRLAQQSQNVQPFTYRAIDPGLFHAVVTQQIPPGPGPQEGRG
                     DPTVHPRGGK"
     gene            1081..>2943
                     /locus_tag="VGC82_05625"
     CDS             1081..>2943
                     /locus_tag="VGC82_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019087776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0422786.1"
                     /translation="MLGKLTWAAIPWEQPIPLIAAAVVGLILLGVIAWVVLKGYLPYL
                     WHDWITSVDHKRIGVMYTLLGLVMLIRGFIDAIMMRSQQALAYNSPGYLPPEHYNQIF
                     SAHGTIMIFFGAMPLVIGLMNFVVPLQLGIRDVAFPTLNSTSFWLTATGALLVNVSLV
                     IGEFARTGWLPYPPLSELSYSPGVGVDYYVWSLQISGVGTLLSGINLVTTILKMRAPG
                     MSYLRMPIFCWTALSSNLLVVAAFPILTATLAMLTLDRYLGFHFFTNTAGGNMMMFMN
                     LIWAWGHPEVYILVLPAFGVFSEVISTFSSKPLFGYRSMVMATLFICLVSFMVWLHHF
                     FTMGAGANVNAVFGIATSVIAVGTGVKVYNWIFTMYGGRIRFATPMLWSLGFIVTFVI
                     GGTTGVLLAVPPADFVLHNSLFLVAHFHNVIIGGVLFAGFAALTYWFPKAFGFRLHEG
                     WGKAAFWFSIAGFYVVFMPLYAVGLLGMTRRLQHYDYAPWRPYMLVAAFGIVIMIAGL
                     ACQIMQLIVSIRQREQLRDLTGDPWNGRSLEWATSSPPPAFNFAVLPNVQGEEPYWEI
                     KRRAIETQQLAAEPDYQAIEMPRNSPTGFITAFFSTFTGFSLIWHIWWLVAVGLV"
BASE COUNT          488 a          916 c          885 g          654 t
ORIGIN      
        1 ggcgacggcg tcgcaatgcg ttgaattgat gtgaaagcaa atttggcggg atggtatgcg
       61 gccccgtagc agtccccaca gcagtctgta gcagtcccca cagcagtcta gagtgcaatg
      121 catatgaaat cggcgcccgc atcatgcacg tgaacccgca ttcgccagcc gcgggcgggc
      181 gatcgcggcg gccggcaccg ttggcagcag cggtctcgtt ggcgctgctc gttcaactca
      241 ctggctgctc ggacggcgtg ctgaacccgc agggaccgat cggcgccgca aacaaactga
      301 tcctgctcaa ctcgttggcg atcatgttgg cgatcgtggt gcccacgatc ctggcggcgc
      361 tggttttcgc gtggtggttc cgcgcctcca ataccagggc tgtctatagg ccggactggg
      421 cctattccgg gcgcatcgag ctgatcgtct ggtccattcc gatcatgatc atcctgtttc
      481 tcggcggcat tacctgggtt ggctcacacg atctggatcc cgcccagccg atcgcatcgc
      541 aaagcaagcc ggtcgaggta caagtagtat cgctcgactg gaagtggctg ttcatctacc
      601 ccgacctgca catcgccagc gtcaaccaac tggtggttcc ggccgcggta ccggtgcatt
      661 ttgcccttac ctccgcgtcc gtcatgaaca gcttcttcgt gccccagctt ggcagcatga
      721 tctacacgat gaacggaatg gtgacgcagc ttcatctgct ggccgaccat gaaggagact
      781 ttttcggcga gtcggccatg ttcagcggcg acggtttttc ggacatgcac ttcatcgtgc
      841 gggcggtgtc ggtcgacgat ttcaccaaat ggacatccac cgtgcagcag acggggccgg
      901 cgctggaccc atccgagtat tcacggctgg cgcagcaaag ccagaatgtg cagcccttta
      961 cgtatcgagc gatcgatcca ggcctgtttc acgccgtcgt gacgcagcaa atcccgccgg
     1021 gcccagggcc gcaggaaggg cgcggcgatc caaccgttca ccccagaggg gggaagtgac
     1081 atgctaggca aacttacctg ggccgctatt ccctgggaac aaccgattcc gctgatcgcc
     1141 gccgcggtgg tgggtctgat cctgcttggc gtgatcgcct gggtcgttct caagggctac
     1201 ctgccgtacc tgtggcacga ttggatcacc agcgtcgatc acaagcggat cggggtgatg
     1261 tacaccctgc ttggactggt gatgctgatc cgcggcttca tcgacgcgat catgatgcgc
     1321 tcgcagcagg cgcttgcgta caactcgcct ggttatttgc cgcccgaaca ttacaatcag
     1381 atcttctcgg cgcacggcac catcatgatc ttctttggcg cgatgccgct ggtgatcggg
     1441 ctgatgaact tcgtggtccc gctgcagctt ggcatacgcg acgtcgcctt tccgacgctc
     1501 aactcgacca gtttctggct taccgccacc ggcgcgctgc tggtcaatgt ttcattggtg
     1561 atcggggagt tcgcgcggac cggctggctg ccgtatccgc ccctgtccga gctcagctac
     1621 tcgcctggcg tgggcgtgga ttactatgtg tggtcgctgc agatctcggg cgtgggcacg
     1681 ctgctgtccg gcatcaatct tgtgacaacg atcctcaaga tgcgggcgcc gggcatgagc
     1741 tatctgcgca tgccgatatt ctgctggacc gcgctgtcgt caaacctgct ggtcgtggcg
     1801 gcgtttccga tcctgacggc aacgctggcg atgctgaccc tggaccgtta cctgggattc
     1861 catttcttca ccaacacggc cggcggcaac atgatgatgt tcatgaacct gatctgggcc
     1921 tgggggcacc ccgaggttta catccttgtg ctgcccgcgt tcggcgtgtt ctccgaggtc
     1981 atatcgacgt tttcgagcaa gccgctgttc ggctaccgct cgatggtcat ggccacgctg
     2041 ttcatctgcc tggtgtcctt catggtgtgg ctgcaccact tcttcacgat gggcgcgggt
     2101 gcgaacgtca acgccgtgtt cggcatcgcc accagcgtca tcgcggtcgg caccggcgtg
     2161 aaggtttata actggatctt caccatgtat ggcggccgta tccgcttcgc gacaccgatg
     2221 ctgtggtcgc tcggctttat cgtgacgttc gtgatcggcg gcacgaccgg tgtgctgctt
     2281 gccgtgccgc cggccgattt cgtgctgcac aacagtcttt tcctggtggc gcacttccat
     2341 aacgtcatca tcggcggcgt gctgttcgct gggttcgcgg ccctcacgta ctggttcccc
     2401 aaggcattcg gcttccggct gcacgaaggc tggggcaagg ccgccttctg gttctcgatc
     2461 gccggcttct acgttgtttt catgccgctg tacgccgtcg gcctgctcgg catgacgcgg
     2521 cgcctgcagc attacgacta cgcgccgtgg cgcccgtaca tgctggtggc agcgttcggc
     2581 atcgtgatca tgattgccgg cctcgcctgt caaatcatgc agctgattgt cagcatccgt
     2641 cagcgcgagc aattgcgcga cctgacgggc gacccgtgga acggacgctc gttggaatgg
     2701 gcgacgtcat cgccgccgcc ggccttcaac ttcgcggtgc tgcccaacgt gcaaggcgag
     2761 gagccatact gggagatcaa gcggcgcgcc attgaaaccc agcaactggc cgccgaaccc
     2821 gattaccagg caatcgaaat gcccagaaac agcccgacgg gcttcatcac cgcgtttttc
     2881 tccaccttca ccggcttttc cctgatctgg catatctggt ggctggtcgc tgtcggcctc
     2941 gtc
//
LOCUS       DASWQN010000343         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1749217, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000343 DASWQN010000000
VERSION     DASWQN010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749217"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1911
                     /locus_tag="VGC82_05630"
     CDS             <1..>1911
                     /locus_tag="VGC82_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY0422787.1"
                     /translation="GYVATCLRTLRENTAYRNFEIICIDNIPPDLVEWKQLIRRDADK
                     IVEIPGKFNWSRFNNRAVEQADGEYLLFLNDDVETQDPNWLDALLEHAQRPEVGIAGP
                     QLLYPDRKVQHAGIFLTTLGSGRHSFRFLAEDDPGYFGLALTQRNVIAVTGACMLMRR
                     DVFDRVGRFDEAHEVINNDVDLCLRVWQSGLSIVYTPYAQLIHHELASRAKIKDIYDS
                     GHFAKQWRTLYAQGDPFLSPRLTKFADEYTPDTEPVRAICASRPLFATKDIQRILAMK
                     LDHIGDLITALPALRRLRRHFPAARIHLLASGAARAFLANEDCVDELIEFEFFHARSG
                     LGQKDVTEADLLALGQRLAPYRFDLALDLRKQIETRHILKHIPARFRAGYNQYDRFPW
                     LDIAVEWEGDFQLRRKHSHISTDLLRLVDVIAAEGDADQQAAPPVAAGDGTLPASLPA
                     AAKALFRKPVVAVHPGVGAIMRQWPPEYFATVIDLLIEKNQVNVVLVGGPDEAALAKV
                     VLGHVVNRRNVASLVGRTSLADLTTVLRSCVLYLGNNSGPQHVAASLGVPTVGIYSGV
                     VDAAEWGPTGPRAIALQRNMICGPCYLVKPSDCVRDMACLKRLEPAVVHQYCEVMLGK
                     VVPESVVAVVLPS"
BASE COUNT          325 a          632 c          620 g          334 t
ORIGIN      
        1 ggctacgtcg cgacctgcct gcgcaccctg cgggagaaca ctgcatatcg caacttcgag
       61 atcatctgca tcgacaacat cccgcccgac ctcgtggaat ggaagcaact gatccgccgc
      121 gacgccgaca aaatcgtcga aatcccgggc aaattcaact ggtcccgctt caacaaccga
      181 gcggtcgagc aggccgacgg cgagtatctg ctgttcctga acgacgacgt cgagacgcag
      241 gacccgaact ggctcgatgc cctgctggag cacgcccagc ggccggaggt cggcatcgcc
      301 gggccgcaac tgctgtatcc cgaccgcaag gtgcagcacg ccggcatctt cctgaccacg
      361 cttggctccg gccgccattc cttccgcttc ctcgccgaag acgatcccgg ctatttcggg
      421 ctggcgctga cccagcgcaa cgtcatcgcc gtcaccggcg cctgcatgct gatgcgccgc
      481 gacgtgttcg accgcgtcgg ccgcttcgat gaggcgcatg aggtgatcaa caacgacgtc
      541 gatctgtgcc tgcgcgtctg gcaatccggc ctgtcgatcg tttacacccc gtatgcccag
      601 ttgatccatc acgagctggc aagccgggcg aagatcaagg acatctacga ttccggccat
      661 ttcgccaagc agtggcgcac gctgtatgcg caaggcgatc cgttccttag ccccaggctg
      721 accaagttcg ccgacgaata cacccccgac accgaaccgg tgcgtgcgat ctgcgccagc
      781 cggccgctgt tcgccaccaa ggacatccag cgcatcctcg cgatgaagct ggaccacatc
      841 ggcgacctga tcaccgcgtt gcccgccctg cgccggctgc ggcggcattt cccagccgcc
      901 cgcatccacc tgctggccag cggcgcggca cgcgccttcc tggctaacga ggactgcgtc
      961 gatgagctga tcgagtttga gttcttccat gctcgttccg gcctgggcca gaaggacgtg
     1021 accgaggcgg acctgctcgc gctgggtcag cgtctggcgc cctatcgctt cgacctggca
     1081 ctcgatctgc gcaaacagat cgagacccgc cacatcctga agcacatccc tgctcggttc
     1141 cgggcggggt acaaccagta cgaccggttc ccctggctgg acatcgcggt ggaatgggaa
     1201 ggcgacttcc agcttcgccg caagcacagc cacatcagca ccgacctgct gcggctggtg
     1261 gacgtgatcg cggccgaggg cgacgccgac cagcaagccg cgccgccggt ggccgccggg
     1321 gatggcacgc tgccggccag cctgcccgcc gcggcgaagg cgctgttccg caagcctgtc
     1381 gtggcggtgc atcccggcgt gggtgcgatc atgcggcaat ggccgcccga gtatttcgcc
     1441 acggtgatcg acctgctgat cgagaagaac caggtcaacg tcgtgctggt gggcgggccg
     1501 gatgaggcgg cgttggccaa ggtcgtgctg gggcatgtgg tcaaccgccg caacgtcgcc
     1561 tcgctggtgg gccgcacctc gttggcggac ctgacgacgg tgctgcggtc gtgcgtgttg
     1621 tatctgggca ataacagcgg gccgcagcat gtggctgcgt cgttgggcgt gccgacggtc
     1681 gggatctact ccggcgtggt ggatgcggcg gaatggggcc caacgggacc cagggcgatc
     1741 gcgttgcagc gcaatatgat ctgcgggccg tgctatctgg tgaagccgtc cgactgcgtg
     1801 cgcgacatgg cgtgtctgaa aagactggaa ccggctgtcg tgcatcagta ctgtgaagtg
     1861 atgttgggga aggtggtgcc ggagtcggtt gtggcggtcg tgctcccgtc c
//
LOCUS       DASWQN010000344         6380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_34175, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000344 DASWQN010000000
VERSION     DASWQN010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34175"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VGC82_05635"
     CDS             complement(<1..346)
                     /locus_tag="VGC82_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HEY0422788.1"
                     /translation="MKVALVNPPWTFDGSIYFGCREPHLPLELGYADALLRRAGHETL
                     LLDCHLCERPVEQACQDIEQFGAEITVVTTAPSYLFWRCAPPELRVPAQFLAALGRRG
                     GRTVAVGPHGSVT"
     gene            complement(343..2355)
                     /locus_tag="VGC82_05640"
     CDS             complement(343..2355)
                     /locus_tag="VGC82_05640"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0422789.1"
                     /translation="MTGSQYGFVEWFRPGEHDRVLASIEGMRAAGASSVRTHLSWAWY
                     HTQGGAAWYDWLIPTISRDFDLLPCVHYTPPSLSRTGRSSGPPGRLKDFPDFVDHVLT
                     RYGDHFRHIELWNEPNNLLDWDWREDPDWLLFCEMIGAAAHWTQQRGWKPVLGGPCPF
                     DPNWLELMGQRGILGTVAAVGFHAFPGTWDSEAGGWAGYDTHLAAMRAVLDRHNPEAE
                     IWITEAGYSTWRNDEAAQVDHFRQALAAPADRFYWYGWQDIPRSVPVQEGLYFDPRHY
                     HLGVTDAAGRPKMLRRLLQTGGVAALRRPAALASPRIVRDIAPVAIFGGAGFIGANLA
                     DALMRDGDDVIVFDNLSRPGVERNLGWLASRHPGHLHFVAGDIRDADAVGAVVRESRS
                     VFHLAAQVAVTASLADPIDDFAVNAQGTLTILEAIRKLAPSVPLVFASTNKVYGALAD
                     LDMEAGPDHYFPAAEHLRDRGVDETRPLDFCTPYGCSKGTADQYVLDYAHSFGLRTAV
                     LRKSCIYGPRQFGTEDQGWVAHFLISVLRGEPITIYGDGKQVRDLLHVSDAVSAYRSV
                     IGNIGKVSGQAFNLGGGVRNAVSLRVVLEEIAAVTGRDVQVSHAEPRTGDQLYFVADT
                     GRLERLVGWQPRVGWREGLRDLAEWIAADLGLPASRIRRPKRSAGA"
     gene            complement(2352..3458)
                     /locus_tag="VGC82_05645"
     CDS             complement(2352..3458)
                     /locus_tag="VGC82_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0422790.1"
                     /translation="MTKTFLLTGGAGFIGTYLCQELLTQGHHVRIIDSLSEQVHGNTG
                     TCLPADVELVRADVRDAQAVASALKGVDGVFHLAAEVGVGQSMYEIARYVGVNNLGTA
                     VLLEQLSRNPIERLVVASSMSVYGEGNYQTPDGTLAQSVQRGIRSDESGWDPVLPDGT
                     RLTPVPTDESKQPDLASIYALTKYSQERSCLIIGSAYGFDAVALRLFNAFGPGQALSN
                     PYTGVLANFGARLLNGQRPIVFEDGQQRRDFVHVRDVAKAFCLAMDSPVAAGRVFNIG
                     SGRVYRIEQVARMLADAMGRSDLAPDIIGKARIGDVRHCFADIGQARDLLGYVPTRFL
                     DNSLDELAEWIAKSTARDRGDHAKRELEAHGLVV"
     gene            3969..4712
                     /locus_tag="VGC82_05650"
     CDS             3969..4712
                     /locus_tag="VGC82_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0422791.1"
                     /translation="MNDVRVIVVGFGDTLVRWQTIMTAPDRLSGYRSKRDFAQTAEPR
                     GEAEIAGERRLRFVVQKHDASRLHYDLRLELDGVFKSWAVTRGPSLDPRDKRLAVEVE
                     DHPLDYGDFEGTIPQGQYGGGTVQLWDRGYWSPEGMDAESGLAAGDLKFTLEGKRLHG
                     SWVLVRMKRDWSRGKRVNWLLIKHKDEFARDGESGAAKSDVGESGAGKSGAGESGDRS
                     IASGRSMADIAAGKGPAPKPFMTSVRPKR"
     gene            4709..5779
                     /locus_tag="VGC82_05655"
     CDS             4709..5779
                     /locus_tag="VGC82_05655"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY0422792.1"
                     /translation="MKLPERPTDSGEAAFLLALDTQPMEAKSVDTLPDEDGWQFEPKW
                     DGFRCLAFKAGNTVDLRAKSGKSLSRYFPEMVAALRAAEPHQFVLDGELAVPAGNTLS
                     FDALQARVHPAASRVARLAAETPALLILFDCLATEEAGSLLDAPLTERRTALEAIAKR
                     LGPGSSARLTPFTRDRQEAERWLMRTHGALDGIVAKRVDHPYVPGERAMLKVKLSRSA
                     DCVVGGFRYAQGSTMVGSMLLGLFNAEGKLDHVGFTSAISNVDRPALTARLEALRGEP
                     GFTGDAPGGPSRWSTERSAAWVPLRSELVVEVSYDHVTAGRFRHGTMLVRWRPDKAPR
                     QCTVEQLQEEARPGRLIEQILR"
     gene            complement(5805..>6380)
                     /locus_tag="VGC82_05660"
     CDS             complement(5805..>6380)
                     /locus_tag="VGC82_05660"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY0422793.1"
                     /translation="FFQRTAPEVASGLIGVTLLVGGVGGIIVETEAYDRDDPASHSFR
                     GRGVRNASMFEPPGCAYVYRSYGIHWCLNAVCGGESAGSAVLIRALEPVAGIDVMCER
                     RKTSDLRQLCRGPGRLCQALGITGVLDGAALDKPPFLLLDRSGPASVSIGPRVGITKG
                     VATPWRFGLSGSLFLSRPIAPRNATPPIDIA"
BASE COUNT         1233 a         1989 c         2005 g         1153 t
ORIGIN      
        1 gcgtgaccga accgtgcggc ccgacggcaa ccgtccggcc gccgcgccgc ccgagcgcgg
       61 caaggaactg ggcaggcacc cgcagttccg gtggtgcgca gcgccagaac aggtagctgg
      121 gcgcggttgt cacgacggtg atctcggcac caaattgctc gatgtcctgg caagcttgct
      181 cgaccggacg ttcgcagaga tgacaatcca acagcagcgt ctcgtggccg gcgcgacgca
      241 gcagggcgtc cgcgtatccc agttcgaggg gcaaatgggg ctcgcggcag ccgaagtaga
      301 tgctgccgtc gaacgtccac ggcggattta cgagagcgac tttcatgcgc ccgcgctacg
      361 cttgggccgc cggatccggc tggctggcaa cccgaggtcg gcggcgatcc attcggcaag
      421 gtcgcgcaga ccttcgcgcc agccaacccg cggctgccaa cctaccaacc tttcgagcct
      481 tccggtatcg gccacgaaat acagttggtc cccggtgcgg ggttcggcgt gcgacacttg
      541 tacatcgcgg ccggtgaccg cggcaatttc ctcgagcacg acccgcagac tgacggcatt
      601 cctgacgcca ccgcccaggt tgaacgcttg gccggaaact ttgccgatat ttccaatgac
      661 gcttcggtag gccgagacgg catcgcttac gtgcagcagg tcgcgcacct gcttgccgtc
      721 gccatagatc gtgatcggct cgccgcgcaa aacgctgatc aggaaatggg cgacccatcc
      781 ttggtcttcg gtgccgaact gacgcggtcc atagatgcag ctcttgcgca gaaccgctgt
      841 gcgaagccca aaggaatgtg cgtagtccag tacgtactgg tcggcggtgc ccttggagca
      901 gccatagggc gtgcagaagt cgagcggacg cgtttcatcg acaccgcggt cccgcaggtg
      961 ttcagccgcc ggaaagtaat ggtctggtcc tgcttccatg tccagatcgg cgagcgcgcc
     1021 atagactttg ttcgtgctcg cgaaaaccag cggaaccgac ggcgccagtt tgcggattgc
     1081 ttccagaatg gtcagcgttc cctgggcgtt gaccgcaaag tcatcgatcg gatcggccag
     1141 gctggctgtc actgcgacct gtgccgcgag atggaacacc gatcgggatt ccctgacgac
     1201 agcgccgacc gcgtccgcgt cgcggatgtc gcccgcgacg aaatgcagat gcccgggatg
     1261 tcggcttgca agccaaccca ggtttcgctc gaccccgggg cgactcaggt tatcgaaaac
     1321 gatgacatcg tcgccgtcgc gcattagcgc atcggcaagg ttggcgccga tgaagcctgc
     1381 gccgccaaag atcgcgactg gtgcgatgtc gcgcacgatc cttggtgagg ccagtgccgc
     1441 cggccgccgc agtgccgcga cgccgccggt ttgcagcagg cggcgcagca ttttgggccg
     1501 gcccgcggca tccgttacgc ccaaatgata atggcgggga tcgaagtaga gtccctcctg
     1561 gacgggcacg gaacggggta tgtcttgcca gccgtaccag taaaacctgt ccgccggtgc
     1621 cgctaatgct tgccggaagt ggtccacctg cgccgcttca tcgttgcgcc aagtggagta
     1681 tcccgcctcc gttatccaga tctcggcctc tggattgtgc cggtcgagca ccgcccgcat
     1741 cgcagccaga tgcgtatcgt agccggccca tccacccgcc tcgctgtccc aggtgccggg
     1801 aaaggcgtgg aaccccacgg ctgcgacggt gcccaggatt ccgcgctgcc ccatcagttc
     1861 aagccagttc ggatcgaaag ggcaggggcc gccgagcacg ggcttccagc cacgctgctg
     1921 cgtccaatgg gctgcagcac cgatcatctc acagaacagc agccagtcgg gatcttcccg
     1981 ccaatcccag tccagcaggt tgttgggctc gttccacaac tcgatatggc ggaaatgatc
     2041 accgtatcgg gttagtacat gatcgacaaa atccggaaaa tccttcagtc gccccggagg
     2101 tccggaggaa cgcccggtgc gtgacagtga cggcggcgtg tagtggacgc acggcaacag
     2161 gtcgaagtct cggctgattg tggggatcag ccagtcgtac caggcggccc ccccttgggt
     2221 atggtaccaa gcccaggaaa gatgcgttcg caccgacgac gcgcccgccg cgcgcatgcc
     2281 ttcgatggac gccaatacgc gatcgtgctc tccgggacgg aaccactcga caaaaccata
     2341 ctggctgccg gtcatacgac caatccatgt gcttccagtt cccgtttcgc atgatcgcca
     2401 cggtcgcgcg cggtggactt ggcgatccat tccgccaatt catcaagcga attgtccaga
     2461 aagcgcgtcg gcacgtagcc cagcaggtcg cgcgcctgtc caatgtccgc gaagcaatgg
     2521 cggacgtcac caatccgggc tttgccgatg atatcgggag ccaaatcgga tcggcccatg
     2581 gcgtcggcga gcatgcgggc cacctgctcg atccggtaaa cccggccgct gccgatgttg
     2641 aagacgcggc cagccgccac tgggctatcc attgccaggc aaaacgcctt ggccacgtcg
     2701 cgaacatgga cgaaatcgcg gcgttgctga ccgtcctcga agacgatcgg gcgctgaccg
     2761 ttcaacagcc ttgctccaaa attcgccagc acgccggtgt aaggattgga caaggcctgg
     2821 ccggggccga acgcattgaa cagccgcagc gcgacggcgt caaaaccata ggcggaaccg
     2881 atgatcaggc aactccgctc ctggctgtac ttcgtgagcg cgtagatcga tgcgaggtcg
     2941 ggctgcttgg attcgtcggt cggcaccggt gtcaggcgcg taccgtcggg cagcaccggg
     3001 tcccagcccg attcatcgct tcttatcccg cgctgaaccg attgtgccag agtgccgtcc
     3061 ggggtctggt aattgccttc gccgtagacg ctcatggatg atgccaccac taaccgctcg
     3121 atcgggttgc ggctgagctg ttccagcagc acggcggtcc caaggttgtt gacgccgaca
     3181 taccgggcga tctcatacat gctttgcccg acgcccactt cggcggccag gtgaaagacc
     3241 ccatcaacgc cttttagcgc ggacgccacc gcctgggcat ctctcacgtc ggcccggacc
     3301 aactcgacat ctgccggcag gcaggttccg gtattgccat gcacttgttc ggaaaggctg
     3361 tcgatgattc gaacgtgatg cccttgtgtc aggagttcct ggcaaaggta tgtcccgata
     3421 aagcccgcgc cgccggttaa caaaaacgtt ttggtcattg gtccagccgt tcccctaaag
     3481 gctctggcca caactcatcc tacggacttg ggttcctgaa gatgaagaaa aagatgtcat
     3541 tgatgaacaa gaagaaggca gcaaagcgac tgatcatccc gatgacgaaa ctctccgctg
     3601 acatcgcaac cacttgatca tcccgcgatc gcacagtctg aacatgataa tgtactagta
     3661 tatcaattgc attggcaagt agccctgcaa caggaacaaa tccgtcacga ctgttgcgat
     3721 tgattcgttg ctttttcggt caaaaattct agtcttattc actttatgaa tagtccggag
     3781 acagcgatat atccggcgcg gattaaatcc gcccgaggcg gtccacacgc gcccttccgg
     3841 cggctggttg gttacgtcca gtgcatgacg tgttgagaat cgcgatgcac cgccgcgcgg
     3901 ctatcaagga tacgttatcg acatagcggc cgacgtggcg cggcgttgtt gcccaacaag
     3961 ggacagggat gaacgatgtt cgcgttattg ttgtcgggtt tggggacacc ctggtccggt
     4021 ggcagaccat catgacggca ccggacagac tctccggcta tcgttcgaaa cgggattttg
     4081 cccagaccgc tgaaccacgg ggcgaggccg agattgctgg cgaacggcgg ctacggttcg
     4141 tggtgcagaa gcatgatgcg tcgcgcctgc attatgacct gcggctggag ttggatggcg
     4201 tcttcaaatc ctgggccgtc acgcgcgggc cgtcgctcga cccgcgtgac aagcgtttgg
     4261 ccgtcgaggt cgaggaccat cctctggact acggcgattt tgagggcacc atcccgcaag
     4321 gccaatatgg cggcggaacg gtgcagcttt gggatcgcgg atactggtcg cccgaaggca
     4381 tggacgccga gtctggcctg gctgccggcg acctgaagtt cactctggag ggcaagcgcc
     4441 tgcatggaag ctgggtgctc gtccggatga aacgcgactg gtctcgcggc aaacgcgtca
     4501 actggctgct gatcaagcat aaggacgagt ttgcccgcga cggcgagtca ggcgcggcca
     4561 agtcagacgt gggcgagtca ggcgcgggca agtccggcgc gggcgagtcc ggggatcgct
     4621 ccatcgcatc cggccgctcg atggccgaca tcgccgccgg aaaaggaccg gcgccgaagc
     4681 cgttcatgac gtccgtccgg ccgaagcgat gaagctgccg gaacgaccga ccgattcggg
     4741 cgaagccgcg tttctgctcg cgctggacac tcagccgatg gaggcgaagt cggtcgatac
     4801 gctacccgat gaggacggct ggcaattcga accaaagtgg gacggcttcc gctgcctggc
     4861 cttcaaggcc ggcaatacgg tcgatttacg ggcgaaatcc ggcaagtcgc tgtcgcgcta
     4921 cttcccggag atggtcgcag ccttgcgggc cgcggaaccg catcaatttg tcttggacgg
     4981 agaactcgcg gttccagcgg gaaatacgtt gtccttcgac gcgctgcagg cgcgcgttca
     5041 tccggctgcc agccgggtcg cgcgactggc cgcggaaacc ccggctttgc tgatcctgtt
     5101 cgattgcctg gccaccgaag aggccggatc gctgcttgac gcgccgctga ccgaacgccg
     5161 gacggcattg gaagccatcg cgaagcgtct cggtcctggt agttcggccc gcctgacacc
     5221 attcacgcgc gaccggcagg aggccgaacg ctggttgatg cggacccatg gtgccctcga
     5281 tggtatcgtc gccaaacgcg ttgatcatcc ttacgtgccg ggtgagcggg cgatgctcaa
     5341 agtgaagctt tcgcgaagtg ccgactgcgt ggttggcggc ttccgctatg cgcaaggcag
     5401 cacaatggtg gggtcaatgc tgctgggact gttcaacgcc gagggcaagc tggaccatgt
     5461 cgggttcacc tccgccatat ccaatgtgga tcgtccggcg ctgacggcgc gcctggaggc
     5521 cctgcggggc gaacctggtt ttactggcga cgcgccgggc ggtccaagcc gctggagcac
     5581 cgaacggtcc gccgcctggg tgccccttcg ctcggaattg gtggtggaag tcagctatga
     5641 ccatgttacc gccggccgct tccgacatgg caccatgttg gtgcggtggc gcccagacaa
     5701 ggcaccgcgg caatgcaccg tcgaacagtt gcaggaggaa gcgcggccgg gccgcctgat
     5761 cgaacaaatc ctgcgatagc agcaaccgcg cgtgacccgc ttcattaagc gatgtcgatc
     5821 ggcggcgtcg cattgcgcgg tgcaatcggc cggctcagga acagtgaccc ggacaggccg
     5881 aagcgccagg gtgtggcaac tccctttgtg atgccgacgc gaggcccgat ggagacgctt
     5941 gccggcccgg atcggtcgag caggaggaac ggcggcttgt cgagagctgc cccgtccagc
     6001 acacccgtta taccgagtgc ctgacaaaga cgaccggggc cacgacaaag ctgacggaga
     6061 tccgacgttt ttcgccgctc gcacatgacg tcgatgcctg ccaccggttc aagcgcccgg
     6121 atcagaaccg cactgcccgc cgattcaccg ccgcagacgg cgttaaggca ccagtggatg
     6181 ccatacgagc ggtaaacata ggcacaaccg ggaggctcga acatcgacgc gtttcggacc
     6241 ccgcgccccc ggaagctgtg cgaggctgga tcgtcccggt cgtatgcctc ggtttcgaca
     6301 ataataccgc cgactcctcc gaccaacagg gtaacgccga tcaggccact cgcgacttct
     6361 ggcgccgtcc gctgaaaaaa
//
LOCUS       DASWQN010000345         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1617304, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000345 DASWQN010000000
VERSION     DASWQN010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617304"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VGC82_05665"
     CDS             <1..300
                     /locus_tag="VGC82_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEY0422794.1"
                     /translation="PKSVTAAVAMGISGRLGGDPALTAVLVILTGIVGAIAVTPLMNG
                     LRITDKRARGFAAGLAAHGIGTARAFQVDPLAGAFAGIAMGLNALLTAILVPLLL"
     gene            complement(359..1225)
                     /locus_tag="VGC82_05670"
     CDS             complement(359..1225)
                     /locus_tag="VGC82_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEY0422795.1"
                     /translation="MIALVDTGRGIVQAVVGPAARRPKTDLSGTALLSTLARSRAGTW
                     FGETAIDGIERMHAFHRVGDRDMAIVVAANWSELMAPADSLGAGTRSLAFIGSAIILL
                     VAGLLLWELHAIRSNRRQKRIFERSRTELKRLRDEEAIQTARSRLHAARLQMVVDSIT
                     DGIALFDSGLRLVQWNGPFIRGIGIHPTQDMPLDTLLREQARKGLFGTVFDLEGETGR
                     RVGILRTGEVTGVSLPGPHGEHLVLRGLPMAECGFMLLLVELDLRRSTPAADETDEPS
                     GAAIATIAVMGR"
     gene            complement(1336..>1552)
                     /locus_tag="VGC82_05675"
     CDS             complement(1336..>1552)
                     /locus_tag="VGC82_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422796.1"
                     /translation="LLAVADDIFICDDKHIIRQDILPKAVGQGVGAAYVTFPHGSLEL
                     FDSDGTKNKESLLLQGQTGRPSIPAGS"
BASE COUNT          310 a          499 c          441 g          302 t
ORIGIN      
        1 ccaaagtcgg tcactgcagc cgtggcgatg ggaatctcag gacggctagg tggcgacccg
       61 gcgttaaccg cggtgctggt gatcctgacc ggcatcgttg gcgctatcgc cgttacgccg
      121 ctgatgaatg gtctgaggat caccgataag cgtgccagag gttttgccgc cgggttggca
      181 gctcatggca tagggaccgc gcgtgccttt caggtggacc cactcgccgg cgccttcgcc
      241 ggaattgcta tgggcctgaa cgcgctgctc acagccatac tcgtcccctt gctgctgtaa
      301 cacgctaaag attgtgctcg cgcccggttc gggtccactc caaaagggct gctgcgtact
      361 accttcccat cacggcgata gtggctattg ccgcaccgga aggttcatcc gtttcatccg
      421 cggccggggt ggaccgtcgt aaatccaatt cgaccagcag aagcataaaa ccgcactctg
      481 ccatcggaag gccacgaagc accaggtgtt cgccatgcgg acccggcagc gacacgcctg
      541 tgacctcgcc ggttcggaga atcccaaccc ggcgaccggt ttccccctcc agatcgaaca
      601 cggtgccaaa caggcccttc ctggcttgtt cccgcagcag agtatcaagc ggcatgtcct
      661 gcgtcggatg aatgccgata cctcggatga acggcccatt ccattgaacc agcctgagac
      721 ccgagtcgaa cagcgcaata ccgtcggtaa tgctgtcgac caccatctgc agtcgagcag
      781 catgcagccg ggatcgggcg gtctggatgg cttcctcatc cctcagacgc ttcaattcgg
      841 ttctgcttcg ctcgaaaatg cgcttttgcc gcctgttgct gcggatggcg tgcagttccc
      901 acaacaatag gccggcgacc aacaggatga tggcggaacc gatgaacgcc aaggagcggg
      961 tgccggcacc aagggaatcc gcgggcgcca tcagttcgct ccagttcgcc gccaccacga
     1021 ttgccatgtc gcggtccccc acgcgatgaa atgcatgcat tctctcgata ccgtcgatcg
     1081 cggtttcgcc gaaccaggta cccgcgcgcg atcgggccag agtgctgagc agtgcagtcc
     1141 cggaaaggtc ggtcttcggt ctgcgggccg ccggccccac aacggcttgc acaatcccac
     1201 gccccgtgtc cacgagcgcg atcaccgaat tatagccgag tgctgcgcca gcaaacagct
     1261 tcgtcagctc ttccgatcgg taagatgctc cgatcagcca tcctttcggg tgatccagtg
     1321 gccgaacgac atacatcagg aaccggcggg tatcgatggc cggccggttt gaccctgaag
     1381 cagcagggat tccttgttct tggttccatc gctgtcaaac agttccagcg agccatgcgg
     1441 aaaggtcaca tacgccgcgc caacaccctg ccctaccgcc ttcggcaaaa tatcttgccg
     1501 gataatatgt ttgtcgtcgc aaatgaatat atcatcggcc accgcgagca gc
//
LOCUS       DASWQN010000346         1849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_429708, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000346 DASWQN010000000
VERSION     DASWQN010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429708"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VGC82_05680"
     CDS             complement(<1..548)
                     /locus_tag="VGC82_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422797.1"
                     /translation="MTVFPDAASWRQGCAGDATLAAWAGPWSVCFAIVSGADTTVFNV
                     IDGTMQPQGGDPAFTLAAPDAVWARFLQPVPPRHHHGIFAMHYRVPEFSIDGDQLVFM
                     QHAHVVRRVLEIGKWLVLGNKPPVPLSLSPRGGKRAVPKVQGGYVPVTLGEVTYQIHH
                     EAAGSGRDVLCMHTAGSDGRQF"
     gene            complement(545..1450)
                     /locus_tag="VGC82_05685"
     CDS             complement(545..1450)
                     /locus_tag="VGC82_05685"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0422798.1"
                     /translation="MSESSAASSSFAPEAACIGFIGLGVMGEPMCGHLVRRSGLSVLA
                     YDLRPEPLERLAAAGVKAASLAEIASDCGVILLSLPDGKAVDAVIGQLEPHLREGQCV
                     VDTSTSPVELTRQIGARLEAKGVLYADAPVARTREAASRGELSIMVGASDDTFARIRT
                     ILETMGSDVTHCGPIGCGQVVKILNNMLVFQHCGALAEAIAIGRRNGVPAAVLLPTMA
                     MGSGDSFVLRNHGMKAMVPGIFPERAFSTRYAIKDLSYALAMAEASGLDVAAAKLTME
                     RLKLAEQAGYGDQYHPVVLKVIDPQ"
     gene            complement(1558..>1849)
                     /locus_tag="VGC82_05690"
     CDS             complement(1558..>1849)
                     /locus_tag="VGC82_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422799.1"
                     /translation="DAAEYWLEVEAKPCVCAPLLPRQRLDVSVGGKWVHSFFALPHGE
                     VGCVVPGHLVAGRKEVEIVLGHPNAASPMLVAGHHDDRRLGAAFYRMALICA"
BASE COUNT          358 a          656 c          551 g          284 t
ORIGIN      
        1 tggaactgtc gcccgtccga cccggcggta tgcatgcaca gcacgtcgcg gcccgatccg
       61 gcggcctcgt gatggatctg atacgtcacc tcccccaggg tcacgggaac atagccaccc
      121 tggacctttg gaaccgcccg cttcccgccc ctgggcgaaa ggctcagcgg caccggcggc
      181 ttgttgccaa gcaccagcca tttgccgatc tccagcaccc gccgaaccac atgcgcgtgc
      241 tgcatgaaca cgagttggtc gccatcgatc gagaactcgg gaacacggta gtgcatcgcg
      301 aagatgccgt gatggtggcg cggcggaacc ggctgcagga acctggccca aaccgcatcc
      361 ggagcagcca gcgtgaacgc cgggtcgccg ccctggggct gcatcgtgcc gtcgatcacg
      421 ttgaacacgg tcgtatccgc cccgctgacg atggcgaaac agaccgacca gggacccgcc
      481 caggcagcca gggtggcgtc gccggcgcaa ccctgccgcc agctcgccgc gtccggaaaa
      541 acggtcattg cgggtcgatc accttgagaa cgacaggatg atactgatcg ccataacccg
      601 cctgttccgc cagcttcagc cgctccatcg tcagctttgc cgccgccacg tccaggccgg
      661 acgcctccgc catcgccagc gcgtaggaca agtccttgat cgcatagcgg gtcgagaatg
      721 cccgttccgg gaaaatcccc ggaaccatgg ccttcatgcc gtggttccgc agcacgaagc
      781 tgtcgccgga acccatcgcc atcgtcggca gcagcacggc cgccggcacg ccgttgcggc
      841 ggccgatggc gatcgcctcg gccaaggcac cgcaatgctg aaagaccagc atgttgttca
      901 ggatcttgac cacctggccg cacccgatgg gaccacaatg ggtgacgtcc gaacccatcg
      961 tttccagaat tgtccggatc ctggcgaagg tgtcgtccga agcgccaacc atgatgctca
     1021 gctcaccgcg cgaggcggcc tcgcgcgtcc gggcgacggg ggcatcggca tagaggaccc
     1081 ccttcgcctc caatcgggcg ccgatctgcc gtgtcagttc aaccggcgag gtgctggtat
     1141 cgacgacaca ttgaccttcc cgcagatgcg gctccaactg cccgatcacc gcatccacgg
     1201 ccttgccgtc cggcaacgac agcagaatga cgccgcaatc cgaggcgatc tccgccaagg
     1261 acgctgcctt cacgcccgcg gccgccaatc gctccagcgg ctcaggccgc agatcgtatg
     1321 ccaggaccga gagacccgaa cggcgaacca aatggccgca cattggctct cccatgacgc
     1381 caagcccaat gaagccgatg caggcggctt ccggtgcaaa ggaggacgaa gcagcggacg
     1441 attcggacat gagggttgcc ttgccgggaa ggcagctgtt ttcgtcacag cccgcccggt
     1501 tgtcaaagcc acccaggacc acgccggcct catatgcgcg atctttgact taccgcatca
     1561 ggcgcagatc agcgccatcc ggtagaatgc cgcgccgagg cgcctgtcgt catggtgtcc
     1621 cgccaccagc atcgggctcg cggcgttggg gtgaccgaga acgatctcga cctccttgcg
     1681 gccggcgacc agatggcccg gcaccacgca acccacctct ccatgtggca gcgcgaagaa
     1741 actatggacc catttaccgc cgaccgagac atccagtcgt tgccgcggca gcaacggcgc
     1801 gcagacgcag ggttttgcct cgacctccag ccaatactcc gccgcatcg
//
LOCUS       DASWQN010000347         4261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_34322, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000347 DASWQN010000000
VERSION     DASWQN010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34322"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1800
                     /gene="leuS"
                     /locus_tag="VGC82_05695"
     CDS             <1..1800
                     /gene="leuS"
                     /locus_tag="VGC82_05695"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY0422800.1"
                     /translation="AIAPEHPLAAAAAAQDPQAADFVAECRSRGTSEATIETGEKLGY
                     DTGLRVRHPFIQGATYPVWIANFVLMEYGTGAIFGCPGHDQRDMDFARKYGLDVIPVV
                     LPPGADPATFTLSNEAYVGPGISFNSGFLNGLEVDAAKRAAIEALEKLGVGQGLTNWR
                     LRDWGVSRQRYWGCPIPIIHCDGCGVVPVPDDQLPVRLPDDVTFDRPGNPLDHHPTWK
                     HVACPVCGKPARRETDTFDTFVDSAWYFARFCSPAADTPLVKAAVDHWMPVDQYIGGI
                     EHAILHLLYSRFFTRAMKDTGHISVEEPFAGLFTQGMVNHESYRGADNRWLYPEEVEK
                     RPDGTVVDRQTGGSVTVGRVEAMSKSKRNTIDPGAIIARYGADTARWFILSDNPPERD
                     MEWTEAGAAGAFRFTQRVFRLAEALDGTRTEMMPDAFGLAGRTLRRATHRCIAAVTEA
                     LESFAFNVAVARLYELANAVTEAERAAPEAGLAWARRESIEMLARLSSPMMPHLAEEV
                     YARLFPDNGHLVAELNWPEAEPALLVAESVTIAVQVMGKLRGTIDVAPNAAAEVVLAA
                     AEADPNVAKALEGKRIVKRVHVPNRIVNFVVAG"
     gene            1797..2330
                     /gene="lptE"
                     /locus_tag="VGC82_05700"
     CDS             1797..2330
                     /gene="lptE"
                     /locus_tag="VGC82_05700"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY0422801.1"
                     /translation="MKHVLSRRLLGLGSLATLGGCGFQPLYMPTASGKPGVAQRELSS
                     VYVGIIPERPGQLLRQALQERFGNDSGTQAAYDLDVTFAIAGEGIAIEQNNLATRIRL
                     IGNATWLLQAHDPKRTALTSGSARALDGVNIFNSQYFAADLQTEAEQKRIAENIATQI
                     ATQLAVWFRQQAAKQTG"
     gene            2336..3361
                     /gene="holA"
                     /locus_tag="VGC82_05705"
     CDS             2336..3361
                     /gene="holA"
                     /locus_tag="VGC82_05705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY0422802.1"
                     /translation="MKLPPARIQAFLRDPGETRVVLLFGDDAGMIRDRAEILVRAVAG
                     SLDDPFLVTELPREDIKQLPNEAAGLSLMGGRRVVRVRDATDAATDPVQALLKTRAAA
                     LVVLEAPTIQSRSRLRTALEAAAEGVAIGCYPEEGRALEETIRETLRANGAGVDPDAL
                     SWLSQQLGADRASTRAELEKLALYVGPGNRVDLDAAMTCVGDLAGLSLDDALFAATTG
                     DVATADRALEAAMAEGAAPVQVLRAALGHLQRLHRARLAMDEQGLNAGDAVKGLRPPV
                     FYQKVGAFTRSLGLWPSPTLTAAMAALAEAERGCKRTGWPDQALCRNAVLTLARRSAV
                     MSRSVRR"
     gene            3498..3875
                     /locus_tag="VGC82_05710"
     CDS             3498..3875
                     /locus_tag="VGC82_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422803.1"
                     /translation="MSIKRPSEQTNPEAASVLPVRPLGSTELVLIQLGISEIAPDWVA
                     ELLGICSEEATVVVLPKTGNDAMGPSFVISRETYKLRVDQIHWDEMTEVGLFTSLDDV
                     LQTLQVRLAFYVAGGTPASGTLH"
     gene            4011..>4261
                     /locus_tag="VGC82_05715"
     CDS             4011..>4261
                     /locus_tag="VGC82_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol efflux MFS transporter"
                     /protein_id="HEY0422804.1"
                     /translation="MMRLPFYSLRGRVHALRGRARAPFLLLVAMTACGTLGMHIIIPA
                     LPATARALGMSIGTSQLTITLYLIGLAIGQLLYGPVSDR"
BASE COUNT          716 a         1350 c         1432 g          763 t
ORIGIN      
        1 gccatcgcgc cggagcaccc gctcgccgcg gcggccgccg cccaagatcc gcaagcggcc
       61 gatttcgtag ccgaatgccg cagccgcggc acctccgaag ccaccatcga aaccggcgag
      121 aagctgggct acgacaccgg cctgagggtc aggcatccgt ttatccaagg tgccacctat
      181 ccggtctgga tcgccaactt cgtgctgatg gaatacggca cgggtgcgat cttcggctgc
      241 cccggccacg accagcgcga catggacttc gcccgcaaat acgggctgga cgtgatcccg
      301 gtggtgctgc cgcccggcgc cgatccggcg accttcacgc tgagcaacga agcctatgtc
      361 gggccaggca tcagcttcaa ttccggcttc ctgaatgggc tggaggtcga tgcggccaag
      421 cgcgcggcga tcgaagcgct cgaaaaacta ggcgtcgggc agggcctgac caactggcgc
      481 ctgcgcgact ggggcgtgtc gcgccagcgc tactggggct gcccgatccc gattatccat
      541 tgcgacggct gcggtgtcgt gccggtgccc gacgatcagt tgccggtgcg cctgcccgac
      601 gatgtaacgt tcgaccgccc gggcaacccg ctggaccacc atcccacatg gaagcacgtc
      661 gcatgtccgg tctgcggcaa gccggcgcgg cgggaaaccg acacgttcga cacgttcgtc
      721 gacagcgcct ggtacttcgc ccggttctgc tcgccggccg cggacacccc gctggtaaag
      781 gccgctgtgg accactggat gccggtcgat cagtatatcg gcggtatcga acacgcgatc
      841 ctgcatttgc tgtattcccg cttcttcacc cgagcgatga aggacaccgg gcatatctcg
      901 gtggaagaac cgttcgccgg cctgttcacc cagggcatgg tgaaccacga atcctaccgc
      961 ggcgcggaca accgctggct ctaccccgaa gaggtcgaga agcgcccgga cggcaccgtc
     1021 gtcgatcgcc agacgggcgg atcggtgacg gtcggccggg tcgaggcgat gagcaagtcc
     1081 aagcggaata ccatagatcc aggcgcgatc atcgctcggt acggtgccga taccgcgcgc
     1141 tggttcatcc tgtccgacaa tccgccggaa cgcgacatgg agtggaccga agccggcgcc
     1201 gccggagcgt tccgcttcac ccagcgtgtt ttccgcctgg ccgaagccct ggatggcacg
     1261 cgcaccgaga tgatgcccga cgccttcggt cttgccggcc gcaccctgcg gcgcgccacc
     1321 caccgctgca ttgccgcggt gaccgaggcg ttggaaagct ttgccttcaa cgtcgccgtc
     1381 gcccgcctat acgaactggc caacgccgtt accgaggccg aacgcgccgc cccggaagcc
     1441 ggcctcgcct gggctcgtcg ggaatcgatc gaaatgttgg cgcgactgag cagcccgatg
     1501 atgccgcact tggccgagga agtgtacgcg cgcctgttcc ccgacaacgg ccatctggtc
     1561 gcggaactga actggccgga ggccgaaccg gcgctgctgg tggctgaaag cgtgaccatc
     1621 gcggtgcagg tcatgggcaa gctgcgcggc acgatcgacg tcgcgccgaa cgccgcggcc
     1681 gaagtcgtgc tggcagcggc cgaggccgat cccaacgtcg ccaaggcgct ggaaggcaag
     1741 cgcatcgtca agcgcgtgca tgttcccaac cgcatcgtca acttcgtggt cgccggatga
     1801 agcacgttct cagccgccgc ctgctcggtc tgggttcgct cgccaccctg ggtggctgcg
     1861 ggttccagcc gctttacatg ccgaccgcgt caggcaagcc cggcgtggcg caacgggaac
     1921 tgtcctcggt ctatgtcggg atcattccgg agcggccggg gcagttgttg cgccaggcgt
     1981 tgcaggagcg gtttggcaat gactccggca cgcaagcggc ctacgacttg gatgtgacgt
     2041 ttgcgattgc cggtgagggc atcgcgatcg agcagaataa tctggccacg cggatccggc
     2101 tgatcggcaa cgccacctgg cttctccagg cgcatgatcc caagcggaca gcattgacct
     2161 ccggcagcgc ccgcgcgctg gacggcgtga acatattcaa ctcgcagtat ttcgccgccg
     2221 acctccaaac tgaggcggag cagaagcgca tcgccgaaaa tatagcgaca cagatcgcta
     2281 ctcagctcgc tgtctggttc cgccaacagg cagcgaaaca gaccggttag gactggtgaa
     2341 acttcctccg gctcggattc aggcgttcct gcgcgatccg ggagaaacca gggtcgtgct
     2401 gttgttcggc gacgatgccg gcatgatccg cgaccgagcc gaaatcctgg tgcgcgccgt
     2461 ggccggttcg ctggacgatc cgttcctggt gacggaattg ccgcgcgagg atatcaagca
     2521 gctacccaac gaagcggcgg gcttgtcgtt gatgggcggc cggcgcgtgg tgcgcgtgcg
     2581 cgacgcgacc gatgcggcga ccgacccggt gcaagccttg ctgaaaaccc gggcggcggc
     2641 gttggtggtg ctggaagcac ctaccatcca atcccgctcc cgattgcgga cggcgctgga
     2701 agccgcggcg gagggcgtgg cgatcggctg ctacccggaa gaagggcggg cgctggagga
     2761 gacgattcgc gagactttgc gtgccaacgg tgccggcgtc gatcccgacg ctttgtcgtg
     2821 gctgtcgcag caactcggcg cggatcgggc atccacccgg gcggaactgg agaagctggc
     2881 gctgtatgtg gggccgggga accgggtcga tctggatgcg gcgatgacct gtgtcggcga
     2941 tctggccggc ctgtcgctgg atgatgcgct gtttgccgcg accacgggcg atgtcgccac
     3001 cgccgaccgg gcgctggaag cggcgatggc cgaaggggca gccccggtgc aggtgcttcg
     3061 cgccgcactc ggtcacctgc agcgtctcca tcgagcgcgg cttgcgatgg acgagcaggg
     3121 attgaacgcc ggcgatgcgg tgaagggtct gcggccgccg gtgttctacc agaaggtcgg
     3181 cgctttcacc cggtcgttag gcttgtggcc ctcgccgacg ctgaccgcgg cgatggcggc
     3241 gttggccgag gcggagcgcg gctgcaagcg caccggctgg ccggatcagg cgctgtgccg
     3301 taacgcggtg ctgacactgg cccgtcgttc ggcggtgatg tctcgttcgg tgcgacgata
     3361 ggcgcgtgct gacgacatat cggagtatta tttagcatta tacgatttat gatgctttgt
     3421 tttgccctcg cactatgcga caacacccgt gttcttgatt ggtttcgggc ggtgggaccg
     3481 caggttcgga gagtccaatg tccatcaaac ggcccagcga gcagaccaat cccgaagcgg
     3541 ccagcgtctt gccggttcgt ccgctcggca gtaccgagct tgtgctgata cagcttggga
     3601 taagcgagat tgcccccgac tgggtggcgg aactgctggg catttgcagc gaagaggcta
     3661 cggtcgtcgt gctgccgaaa accggcaatg acgcgatggg ccccagcttc gtgatcagcc
     3721 gcgagaccta taagcttcgg gtggatcaga tccattggga tgagatgacc gaggttggcc
     3781 tgtttacttc gctcgacgat gttctgcaga ctctgcaggt tcgcctggcg ttctacgtcg
     3841 ccggcggcac gcccgcctcg ggcacgctgc attaggacgc tttcagactg gctggatgca
     3901 gccagtttgc cgccttctgg cgggccgaca gatcgccaat cgtttggcga ttgatccctg
     3961 attgccggtc gtgcggcagc acggcatggt ggcgtccgac gtcgggaaag atgatgcgac
     4021 ttccatttta ttcgctgcgc gggcgggtcc atgcgctgcg cgggcgggcg cgtgcgccgt
     4081 ttcttttgct cgtcgcgatg accgcatgtg gcacgcttgg catgcacatc atcatccctg
     4141 ccttgcccgc cacggcacga gcgttgggga tgtcgatcgg gacgtcgcaa ctgacgatca
     4201 cgctttacct gatcggtctg gcgatcggcc agttgctgta cggccccgtg tcagaccggt
     4261 t
//
LOCUS       DASWQN010000348         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1749458, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000348 DASWQN010000000
VERSION     DASWQN010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749458"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..932)
                     /locus_tag="VGC82_05720"
     CDS             complement(150..932)
                     /locus_tag="VGC82_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY0422805.1"
                     /translation="MPDLIETIEDGVATLTLNRPENLNALSEEIRLGLLESIHRLGAD
                     DSVGCIVLTGAGRGFCAGGDVKTMAGRSSRGFEERAAGIQQSNRIPMLMHTTPKVIIG
                     MINGVAVGAGLSMAAACDLRVAGRSARFGTGFIKIGLSGDWGGTWTLTRLVGTAKARE
                     LFFTGDLIDADEALRIGLVNKVVDDADLSQATMTLAHRIASMPRVALGYTKRNLAVAE
                     TGEFATSLDMEAFNQARCSQTADHREAVQAFKEKRRPVFEGR"
     gene            complement(1022..1366)
                     /locus_tag="VGC82_05725"
     CDS             complement(1022..1366)
                     /locus_tag="VGC82_05725"
                     /inference="COORDINATES: protein motif:HMM:NF021200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-opening amidohydrolase"
                     /protein_id="HEY0422806.1"
                     /translation="MSCDEIDLPAVIGALTDAELPPVGQLTDAQRGNLLAVPGKADPA
                     SIGNIRGARHIMLDDSDINATRHARALVGGVIAGIVGRTDLFVSGGAEHQGPDGVALS
                     RSSLHARPCKAD"
     gene            complement(1353..>1881)
                     /locus_tag="VGC82_05730"
     CDS             complement(1353..>1881)
                     /locus_tag="VGC82_05730"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEY0422807.1"
                     /translation="TIGIREPGFVWLARIRIASFLPATVLDAYADETGLLEVRLLDSI
                     RLARISGSNISRGELMRYLAELPWAPHAILHNRHLSWHELDRNTMEVSAPCVGGSARV
                     RLLFEAGDITGIEADDRPRAAAGRTIPTPWRGRFFDYRTIGGCRIPTRAVVGWLLDDG
                     PFDCWRGAITNYELR"
BASE COUNT          348 a          606 c          599 g          328 t
ORIGIN      
        1 aattgcaact actctgtaac cgaggcggga aattgccgcc cctcggaagc ggtcggaaca
       61 gccgcacagg gacttccggt gacggcgcgg ccaggaaaac cccgtttatg gacggacact
      121 agctagatca acgccctcga acacccaaat cagcgccctt cgaacaccgg ccggcgcttt
      181 tccttaaacg cctgcacggc ctcgcggtga tccgcggtct gggaacagcg agcttggttg
      241 aacgcctcca tatcgaggga ggtggcgaac tcgcctgttt ccgcgacagc gagattcctt
      301 ttcgtgtagc ccagggcgac gcgcggcatc gaggcgatcc tgtgcgccag cgtcatggtg
      361 gcctgcgaca ggtcggcgtc gtccaccacc ttgttgacca agccgatccg caaagcctca
      421 tcggcatcga tcaggtcccc ggtgaagaac aattcgcggg ctttcgcggt gccgaccagg
      481 cgggtgaggg tccaggtgcc gccccagtcg ccggacaggc cgatcttgat gaagccggtg
      541 ccgaaacggg ccgatcgtcc tgccacgcgc aggtcgcatg cggcagccat gctgaggccg
      601 gcgccgaccg cgacaccatt gatcatgccg ataatgacct tgggcgtggt gtgcatcagc
      661 atcggaatgc ggttggattg ctggatgccg gcagcgcgtt cctcgaatcc gcgggaggat
      721 cggccggcca tggttttcac gtcgccgccg gcgcagaagc cgcgcccagc gccggtcagc
      781 acgatgcagc cgacggaatc atcggctccg agccgatgga tcgattccag caggcctaga
      841 cggatttcct cggacaaggc gttcaggttc tcaggccgat tgagcgtcag ggtcgcgaca
      901 ccgtcttcga tggtctcgat caggtcgggc atgcacgtgt tctccgctcg tctgtcacgg
      961 tagcttggct tggcgcgctg atcgccgcaa cagcggggca tgaaacaagc cgttgacccc
     1021 ttcaatcagc tttgcagggc cgagcgtgca acgatgaccg cgacagggcc accccatcgg
     1081 gaccttgatg ttcggcgcca ccggaaacga acaggtctgt ccgtccgacg atcccggcaa
     1141 tgactccgcc caccagcgcc cgggcgtgcc tggtggcatt gatgtcgctg tcgtccagca
     1201 tgatgtggcg ggcgccgcga atgttcccga tcgaagcggg atcggccttg ccgggcacgg
     1261 ccagcaggtt gccgcgctgt gcatcggtca gttgccccac cggcggcagt tcggcatccg
     1321 tcaatgcacc gatcaccgcc ggcagatcga tttcatcgca gctcatagtt ggtgatggcg
     1381 ccgcgccagc aatcgaacgg accgtcgtcc aacagccagc ccacgacagc ccgtgtggga
     1441 atgcggcagc cgccgattgt ccggtagtcg aagaagcgcc cccgccaagg tgtgggaatg
     1501 gtgcgtccgg cagccgctcg ggggcggtcg tcggcctcga tgcccgtgat gtcgcccgct
     1561 tcgaacaaca accgtactct ggctgacccg ccaacgcatg gcgcggacac ctccatcgtg
     1621 ttccggtcaa gttcgtgcca ggacaaatgc cggttatgca ggattgcatg gggcgcccat
     1681 ggcagttccg ccagatagcg catgagttcg ccccggctga tattggaccc gctgattcgg
     1741 gcaagccgaa tggaatccaa caaccgcacc tccagcagcc cggtttcatc cgcgtaggca
     1801 tcgagaacgg tggcaggaag gaacgacgcg atccgtatgc gcgccagcca gacgaagcct
     1861 ggctcgcgga tgccgattgt c
//
LOCUS       DASWQN010000349         3672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_297881, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000349 DASWQN010000000
VERSION     DASWQN010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297881"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3120)
                     /locus_tag="VGC82_05735"
     CDS             complement(<1..3120)
                     /locus_tag="VGC82_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422808.1"
                     /translation="MRRLAKWGAAIVLIVLAIPVVGVAIALALANVDPGRRFIEREID
                     SLTGGMVRIQDLAGRFPDALRIGQIEVLDAKGPYVTVSALTLDWSPLKLTRRIARIDL
                     LQAERLEFSRLPESGSKKTSSGGSYNLPVQIDLGRLHVDQAVIGAPVAGVAAILALDG
                     SAQLPTLSEGTVQLDVRRLDSPGHYAVNGRVTSADIRASIGANEPAKGLISSVAHLPD
                     LGAIQIQASVNGPRDALATQAGITAGLLTASASGTLDLDHQAADMAVQARAPAMAPAP
                     GVSWQSVSVDAKVHGPFTRPDANGTIKIASLNAAGARIGALDADVSGNAGRVGVLATV
                     NDLHVPGPKPDIFAAAPVTLDLAAQLDAPDRPVTFALHHPLISADGSARTEGVQQVQA
                     HLLLANLSALAASAGTDVPGTDVQGDADLNITASIQNDTTTAEVNGRVGITGGMAPVP
                     ALIGEDGRIDLAATMRGPDIALSRLTVNGKALDLSAHGGLADQVVDLDWTVGLADLSV
                     LQPSLSGKLGAIGHAGGKLDDLSVHANIGADLASKGYSSGHIAATVDATGLPAAPRAA
                     IQANGTLLDAPLTLALTAEQANGAIKADIGQLTWKSLQAAGALSLTPPATIPVGHLHL
                     GLGRLADLEPLLGRPVSGQADVTLDSDDQAAKLSLAMRDVALPGTAAFSKAALNAIIA
                     DPAGQPAVDGIFTIDGVSTGTVKAMSGRVTAKGTMEALGITAAVDAPSAAGGPAKVVT
                     GGTLNARDRTLLLARLEASWKQQVLKLLAPARFNFADGVAMDRVRLGFRQAELNVSGS
                     AGSKLDLTASLRNLPADIAAIVNPAFAADGTITADVRLTGTSARPEGTVKLTATGIHQ
                     RQGPGQALPAANLVANANLLGTSVRLDSKLTAGPSQVSVAGLAPLSQGGELNLKTDGR
                     IDLAMLDPLLMAQGRRARGELTIDAAITGTTAAPLIRGTAELRKGDVADYALGARLND
                     LAATIQASGDTIRLASFRGKAGPGSVGGSGSISLAGTMPVDLRFTADDARPLTSDLLS
                     ALIDASLTVQ"
     gene            complement(3137..>3672)
                     /locus_tag="VGC82_05740"
     CDS             complement(3137..>3672)
                     /locus_tag="VGC82_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY0422809.1"
                     /translation="GTRATLLVTPTMSLGTPSSTFLITQLSGSKYLDVFNDGRSVVAL
                     RGLVGQASGVGVFGLPPDQRFYAGGSGTVRGYRYQTLGPQFPDRKPTGGTAISTGTLE
                     LRQRILGSYGIVGFVDVGQVSANGAPFTSNWHAGAGVGARYYTPIGPIRLDVAVPLSK
                     LPGGDTFELYIGIGQAF"
BASE COUNT          645 a         1189 c         1209 g          629 t
ORIGIN      
        1 ctgaaccgtc aggctcgcat cgatcaaggc ggacagcagg tcgctggtca gcggccttgc
       61 atcgtcggcg gtaaaccgta agtcaaccgg cattgtgccg gccaggctga tcgagccgct
      121 gccgcctacc gagccgggcc cggcttttcc cctgaagctg gcaagccgga tcgtgtcgcc
      181 tgaagcctgg atcgtcgctg ccaggtcatt gagacgagcg cccaacgcgt agtccgcgac
      241 gtcgcccttg cgaagttcgg cggttccacg gatcaacggg gcggcggtgg tcccggttat
      301 agcggcgtcg atggtcaatt ctccacgggc ccggcggcct tgcgccatca gcagtggatc
      361 gagcatcgcc aggtcgatcc ggccgtcggt tttcaaattc aactcgccgc cttgcgacaa
      421 cggggccaaa cccgcaaccg aaacctggga aggtcctgcg gtcagcttgc tatcgaggcg
      481 aaccgacgtc ccgagcagat tggcgttggc gacaagattg gcggcgggga gtgcctggcc
      541 ggggccttgg cgttgatgga tgccggtcgc tgtcagcttg acggtgccct ccggacgggc
      601 actcgtgccg gtcagccgaa catcggccgt gatcgtgccg tcggcggcga acgcggggtt
      661 gacgatagcc gcgatgtccg ccgggaggtt tcgcaggctg gcggtcaggt ccaacttgct
      721 gcccgcgcta ccggacacgt tcagttcggc ttgtcggaaa cccagccgaa cccggtccat
      781 cgcgacgcca tcggcgaagt tgaacctggc cggggccaga agcttcagca cctgttgctt
      841 ccagcttgcc tccagacgag ccagcagcag cgtccggtcg cgagcgttca acgtgccgcc
      901 ggtgaccacc ttcgccggac cgcccgcggc agatggcgca tcaaccgcgg cagtgatgcc
      961 gagcgcctcc atggtgcctt tggcggtgac gcggcccgac attgctttca cggttccggt
     1021 ggacacgcca tcgatcgtaa agattccgtc caccgcgggt tgtccggcgg gatcagcgat
     1081 aatggcattc agggcggcct tgctgaatgc ggccgtcccc ggcaatgcca cgtcgcgcat
     1141 tgccagcgac agcttcgccg cttggtcgtc agagtccagc gtgacatcgg cttggccgga
     1201 gaccgggcgg cccagaagcg gctccagatc cgcaagacgg ccgagaccga ggtgcagatg
     1261 gcccaccggg atggttgcgg gcggagtcag gctgagcgca cccgcggctt gcagcgactt
     1321 ccaggtcagc tgaccgatgt cggctttgat cgctccattg gcttgttcgg cggtgagcgc
     1381 gagcgtcaac ggtgcgtcca gcagagtccc gttcgcctgg atcgcggcac ggggggcggc
     1441 gggcaatccg gttgcatcca cagtggcggc gatatggccg gacgagtagc ccttcgacgc
     1501 gaggtcggca ccaatgttgg cgtgtacact caggtcgtcc agctttccgc cggcgtggcc
     1561 aatggcaccc agcttgccgg acaggctggg ctggagaacg gacagatcag ccaaaccaac
     1621 cgtccagtcc agatcgacga cctgatcggc gaggccgcca tgagccgata agtccaacgc
     1681 cttgccgttc accgtcagcc gcgaaagcgc gatgtccggt ccgcgcattg tggctgccag
     1741 gtcgatgcgt ccgtcttcgc cgatcaacgc gggaaccgga gccatgccgc cggttatgcc
     1801 cactcgtccg ttgacctcgg ccgttgtcgt gtcgttttgt atcgacgcgg tgatattcag
     1861 gtccgcatcg ccctgtacat cggtgccggg tacatcggtg ccggcggacg ctgccagcgc
     1921 cgacaggttg gcgaggagca aatgcgcctg gacctgttgg acaccctcgg tccgggcgga
     1981 cccgtcagct gaaatcaaag ggtggtgcaa cgcgaaggtc acgggacggt ctggcgcatc
     2041 cagttgcgca gccaggtcca gggtcaccgg ggcggcagcg aagatatccg gcttcgggcc
     2101 gggcacgtgc aggtcgttca ccgtcgccag cacaccgacc cggcccgcat tgccgctgac
     2161 gtcggcgtcc agagcgccga ttcgggcacc tgcggcgttg agcgaggcga tcttgatcgt
     2221 gccgttcgcg tccggcctgg tgaacggtcc atgcaccttg gcgtccaccg atacggactg
     2281 ccaggacact ccgggagccg gcgccatggc cggagcccgg gcttgcaccg ccatatccgc
     2341 ggcctgatga tccaggtcca aagtgccgga ggccgaagcg gtaagaagcc cggcggtgat
     2401 gccggcctgc gtcgccagtg cgtcccgtgg accattgacg gaggcctgga tttggatcgc
     2461 gcccaggtcg ggaaggtgcg cgacgctgga aatcagtcct ttggcaggtt cattggcccc
     2521 gatcgaagct cggatatccg cggatgttac gcgaccgttg acggcataat ggccgggact
     2581 gtccaggcgt cgaacatcca gttggacggt tccttcactg agcgtgggaa gctgcgccga
     2641 tccgtccagg gccaggatgg ccgccacgcc ggcgacgggc gcgccaatca ccgcctgatc
     2701 gacatgcagg cgacccagat cgatctgcac cggcagatta tacgatccgc cgctgctggt
     2761 tttcttgctt ccggactccg gcaacctgct gaactcgagg cgttcggcct gcagcaggtc
     2821 gatcctggcg atccgccgcg tcaatttcag cggcgaccag tccagggtga gggcggaaac
     2881 cgtgacgtag ggacctttcg cgtccaacac ctcgatctgg ccgatccgca gcgcatcggg
     2941 aaagcgccca gccaggtcct ggatccgcac cataccgccg gtgaggctgt cgatttctcg
     3001 ctcgatgaag cggcggccag gatcgacgtt ggccaaagcc agggcgatag cgacccccac
     3061 cacggggatc gccaatacga tcaggacgat cgctgcgccc cacttcgcca aacgccgcat
     3121 caggacgggt gccgggtcaa aatgcctgtc ctatcccgat gtacaactca aacgtatccc
     3181 cgcccggcaa cttgctcagc ggcaccgcga cgtccagccg gatcggcccg atgggggtat
     3241 aataacgcgc gccgacacct gccccggcat gccagttgct ggtgaatggc gcgccattcg
     3301 cgctgacctg tcccacatcg acgaatccca cgatcccata gctgccaaga atacgttggc
     3361 gcaactccag agttccggtc gagatggcgg tgccgccggt cggcttccga tccggaaatt
     3421 gcggacccag cgtttggtac cggtagccgc gaaccgtgcc gctgccgccg gcatagaagc
     3481 gctggtcagg cggcaagccg aacacgccga cgccggatgc ctggccaacc aggccgcgca
     3541 gcgccacaac actgcggcca tcattgaaaa cgtcgaggta cttcgaaccg gaaagctgcg
     3601 tgataagaaa ggttgaactc ggagtgccga gtgacatggt tggcgtgacc aacaatgtcg
     3661 cccttgtgcc gg
//
LOCUS       DASWQN010000350         3148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_166006, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000350 DASWQN010000000
VERSION     DASWQN010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166006"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3148)
                     /locus_tag="VGC82_05745"
     CDS             complement(<1..>3148)
                     /locus_tag="VGC82_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0422810.1"
                     /translation="SAEAQSLEQARTIARLVDREIERAEAVAETLAAAVPAVRGDLEA
                     LEGELRTAQRLLAANLLPDAPLPTVTLLDARGHLLLHTAWAPAERRQGLPGTQLGLTA
                     ITQGRPQLSDLFFEPGSHAAMLALAVPIFAAKAAAPGREIVGAIGITIPRERMIAIVN
                     EVAMPSGAVASIHDRKGVIVARSFRDKETVGKSPVPAALDAILAAPSGYAPRNTTTLE
                     NVPSFMAFAHAPNSNYVVAINLPEATVFAPLRESLIRSAAIGTLMLAAGLGLAVFRAR
                     DIVRAFRLTLAGAANGLRERPRKSSRLREADELASLLATAFAERERATASARALFENS
                     PIGMVIFDTEGRVHEANDAFLAMVGQTRSELEAGPLNWDDMTPASRLPADEAAFAEAL
                     AVGKCTPYEKQCRHSDGHLVPVLMSFGLTDRASGLAAAFIVDLSEQRKSEAAYRETEE
                     RLNFSLQAGRLGAWELDLASQRIHCSKLAQALYGRVLDGDVSLTQLVSAVHPDDRDRL
                     LGAIARAGLGHEEFRIEYRVIWPDESIHWIEVRGRVLHEDGKSTRLAGVSADITERKQ
                     AEAALRESEMRLRAITDTMPQMVWSTRPDGYHDYYNQRWYELTGVNSGQTEGAGWKTI
                     IHPDDRDRVGTRWQHSLLTGEAYEIEYRLRMANGDYRWVLGRALPIEDPQTGRISRWF
                     GTCTDIEETVAARETLARSREELEQLVAERTRDLQTTQTRLAQAQRMEALGQLAGGIA
                     HDFNNVLQAVQGSAALLERTPADVAGVRRLSRRILAVTERGAAVTERLLAFSRRGDLR
                     AEAVDPAAMHAGLRDILSHTLGDGVKVTLELEPEVPSLLADKRQLETVLVNLATNGRD
                     AMSGIGVLTLAAASEMVAQHQAAGHRVGLKAGFYVRVSVTDTGTGMDARTLARASEPF
                     FTTKPTGKGTGLGLAMARGFAEQSGGGMHIASAPRQGTTVSLWFPVAPDGASESLAPA
                     DSAIQTTAKQGYARVLLVDDDEIVRQVTAELMEASGFAVLSVASAAAALAVVGGDAAV
                     DVLISDLSMPEMDGVSLIK"
BASE COUNT          516 a         1075 c          974 g          583 t
ORIGIN      
        1 ccttgatcag ggacacgccg tccatttccg gcatagaaag gtcggaaata agcacgtcca
       61 ccgccgcatc gccgccgacg accgccagtg ccgccgccgc ggaggctacc gacagcacag
      121 cgaagcccga ggcctccatc aattcggcag taacctggcg aacgatctca tcgtcgtcca
      181 ccagcaatac gcgggcataa ccttgtttcg cggtggtctg tatcgcgctg tcagccggcg
      241 cgaggctttc ggacgctcca tccggggcaa cgggaaacca cagcgaaacc gttgtacctt
      301 gccgcggggc gctcgcgatg tgcatcccgc cgccgctttg ttcggcgaag ccgcgggcca
      361 tggctaagcc aagtcctgtt cccttccccg tcggtttggt cgtgaagaac ggctcggatg
      421 cccgcgccag tgtccttgca tccataccgg tgccggtgtc cgttaccgat actcgcacgt
      481 agaagcccgc cttcaggccg acgcggtgcc cggcggcctg gtgttgggcc accatttcgc
      541 ttgctgccgc caatgtcagc acgccgatgc cggacatcgc gtcccggccg ttcgtcgcga
      601 gattgaccag taccgtttcc agttggcgtt tgtcggccaa cagcgacggc acctccggtt
      661 caagttcgag cgtgactttc acgccatcac ccagcgtgtg cgacaggatg tcccgcaaac
      721 ccgcatgcat cgctgccggg tccaccgctt ccgcccgcag gtcgcctcgg cgcgagaaag
      781 ccagcaaacg ttcggtgacc gcggcgccac gctccgtcac cgcgagaatg cgccgcgaaa
      841 ggcggcgaac cccggcaacg tccgcggggg ttcgttcgag cagcgcggcg ctgccttgca
      901 ccgcctgcag aacattgttg aagtcgtgcg cgatgccgcc cgcgagttgc cccagcgcct
      961 ccatacgctg tgcctgagcg agcctcgtct gggtcgtttg caggtcccgc gttcgttccg
     1021 cgaccagttg ttcgagttcc tcccgcgagc gcgcgagcgt ctcgcgtgcc gcgacagttt
     1081 cctcgatgtc ggtgcaggtg ccgaaccagc gcgatatccg cccggtctgc gggtcctcga
     1141 tcggcagggc tcggccgagc acccagcggt agtcgccgtt tgccatgcgc agccggtatt
     1201 cgatctcata agcttcgccg gtgagcagcg aatgctgcca acgtgtccca acacgatccc
     1261 ggtcatccgg atgaatgatg gtcttccagc cggcgccttc ggtctgcccg ctatttacgc
     1321 cggtcaactc gtaccagcgt tgattgtagt agtcgtgata gccgtccggc cgcgttgacc
     1381 acaccatctg cggcatcgtg tcggtgatcg cccgcagccg catctcgctt tcccgcagtg
     1441 ccgcttccgc ttgcttgcgt tcggtgatgt cggccgacac gccggccaac cgggtggatt
     1501 tgccgtcctc gtgcagcacc ctgccgcgca cttcgatcca gtggatgctt tcgtcgggcc
     1561 aaatcacccg atactcgatg cggaactcct cgtggcccaa gccagcgcgc gctatcgcgc
     1621 ctagcaggcg atcccggtcg tcgggatgaa ccgccgaaac aagttgtgtc agcgatacat
     1681 ccccgtccag cacgcggcca tacagcgcct gagccagctt cgagcagtga atcctttggc
     1741 ttgccagatc gagctcccat gccccgagcc gtcctgcctg caaggagaaa ttcagtcgct
     1801 cctcggtttc gcgataggcc gcctcgcttt tccgctgttc gctcagatcc acgatgaacg
     1861 ccgcggccag gccgctggcg cggtcggtca atccgaacga catcaacacg ggcacaagat
     1921 gcccatcgga atggcggcat tgcttctcgt acggcgtgca ctttcccacc gccagcgctt
     1981 cggcgaaggc ggcctcatcc gccggaagcc gcgacgccgg cgtcatatca tcccaattga
     2041 gcggtcccgc ttccagttcg ctacgggttt gaccaaccat agccagaaac gcatcgttcg
     2101 cctcgtgcac gcggccttcg gtgtcgaaga taaccatgcc gatcgggctg ttttcgaaca
     2161 gcgcccgggc gcttgcggtt gcgcgttcgc gttccgcgaa ggcggtcgca agcagtgatg
     2221 cgagttcgtc ggcttccctc aatctggagg actttcgcgg cctttccctg aggccgttcg
     2281 ctgcgccggc gagcgtgaga cgaaaagccc ggacgatgtc gcgggcgcgg aagacggcca
     2341 gcccgagccc ggcggcaagc atcagggtgc cgatcgcggc agaccggatc agcgattcac
     2401 gcagtggtgc gaagaccgtg gcttcgggaa ggtttatcgc aacgacgtag ttgctgttcg
     2461 gggcatgtgc gaaagccatg aaggacggaa cgttttccag cgtcgtggtg ttgcgcggcg
     2521 cgtagcccga cggggcggcg agtatcgcgt ccaacgcggc cggcaccggc gatttgccca
     2581 ccgtttcctt gtcccggaag gagcgagcca cgatcacccc cttgcggtca tggatcgagg
     2641 ccaccgcacc ggacggcatg gccacttcat tgacgatggc gatcatccgc tcacgcggta
     2701 tggtgatgcc gatggctcct acgatctcgc gccccggcgc ggccgctttc gccgcgaaaa
     2761 tgggcaccgc gagagccaac atggcagcat gcgaacccgg ttcgaaaaac aggtcggaca
     2821 gttgcggccg gccctgggtg atggcggtca acccgagttg cgttccgggc aagccttgtc
     2881 gccgctctgc tggtgcccag gccgtatgca gaagcaagtg cccccgcgcg tccagcaaag
     2941 tcaccgtcgg cagcggcgca tctggcagta gattggccgc gagcaggcgc tgggcggtgc
     3001 gcagttcgcc ttccaacgct tccaggtcgc cccgaacggc cggaacggcg gcggccagcg
     3061 tttccgccac cgcctcggct cgctcgattt ctcgatccac cagccgggcg atggttcgcg
     3121 cctgctcgag cgattgcgcc tccgcgct
//
LOCUS       DASWQN010000351         2784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1221716, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000351 DASWQN010000000
VERSION     DASWQN010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221716"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VGC82_05750"
     CDS             complement(<1..1317)
                     /locus_tag="VGC82_05750"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0422811.1"
                     /translation="MTLTRSLGRFVAELSPNRLPEEAARIARMGFIDTIGTMIAGRNE
                     DSVRIMTETLAPADGLATLTFGDRHAPAPEAAWINGTAAHALDYDDVALRGHPSTVLV
                     PAILAEAEHLGSSGADMITAYVAGYETWAELFRRDTGLLHQKGWHPTGLYGAVGAAAS
                     CAKLRKLDAEQCAIAIALGASQSAGLMSNFGTMTKPFHAGKAAHAGIMAARLAQAGFT
                     ANTDALEHPQGFLHAISPTDTEDRVSESKAGTEWAILTQGLGIKKYPTCYCTHRAIDC
                     MLDLVNNSPIVAENVEKITVSISDYFSTVLRNHQPDTGLAAKFSIEFCMASGIVAKRV
                     GLRELTDGFVQRPDIQALMKKVEIVTTSAYDPELPGAAAHDQVTVTLANGKTLEGEPV
                     ARATGHPSRPLTDQQIFEKFADCLDAGESPIPADVLFERLSAIQSIN"
     gene            1596..2021
                     /locus_tag="VGC82_05755"
     CDS             1596..2021
                     /locus_tag="VGC82_05755"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY0422812.1"
                     /translation="MFRQSLVLAGFAVVFAGAASAATTKYHAALSPTAEVPPTKSTGS
                     GEATASLDTATHELTYDVTFKGFSTDVTAAHIHGPADATKNAGVVLPLGTAPKSPIHG
                     TAKLTPEQEQQLSAGMMYVNVHTKDDPSGAIRGQLVQAK"
     gene            complement(2075..2557)
                     /locus_tag="VGC82_05760"
     CDS             complement(2075..2557)
                     /locus_tag="VGC82_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0422813.1"
                     /translation="MRHFTSCVVASLAVSICMTTGIALAADSPMALFPNPAALKWSPA
                     PPMVPKGAQISILSGDPTKAGPFIIRLKLPANYAIPAHHHPTTENVTVLSGTLYAGMG
                     DKLDRSASKPFAAGGFAGMPAEMNHFAWTKTPAVIQVEAEGPFAFTYANPADDPSKVR
                     "
BASE COUNT          492 a          854 c          915 g          523 t
ORIGIN      
        1 attgatcgac tggatcgccg acaggcgctc aaacaacacg tcggccggga tcggcgattc
       61 tccggcatca aggcagtcgg cgaacttttc aaagatctgc tggtcggtca gcgggcgaga
      121 cgggtggccg gtcgcgcgtg caaccggttc gccttccagc gttttgccgt tcgccagtgt
      181 caccgtgacc tggtcgtgtg cggcggcacc cggcagttcc gggtcatagg cgctggtggt
      241 gacgatctcg accttcttca tcagcgcctg gatatcggga cgctggacga agccgtcggt
      301 cagctcccgc aggccgaccc gctttgccac gatgccgctg gccatgcaga actcgatgct
      361 gaacttggct gccagaccgg tatcgggctg gtggttgcgc agcacggtgg agaaataatc
      421 gctgatgctg acagtaatct tctcgacgtt ttccgccacg atcgggctgt tgttcaccag
      481 atccagcatg cagtcgatgg ctctgtgggt gcagtagcag gtggggtatt tcttgatccc
      541 caggccctgg gtcaggatcg cccactcggt gccggccttg ctttcgctga cgcggtcttc
      601 cgtgtcggtc ggggagatgg catgcaggaa gccctgcggg tgctccaatg cgtcggtatt
      661 ggcggtgaag cccgcctggg cgaggcgagc ggccataata ccggcgtggg cggcttttcc
      721 ggcgtggaac ggcttggtca tggtgccgaa attggacatc aggccggcgg attgggatgc
      781 cccgagggcg atcgcgatgg cgcattgctc cgcgtccagc ttacgcagct tggcacagga
      841 agccgccgcg ccgaccgcgc cgtacaggcc cgtggggtgc caacccttct ggtgcagcag
      901 cccggtgtcg cgccggaaca actccgccca ggtctcgtag ccggcgacgt aggccgtgat
      961 catgtcggcc ccggaggatc ccaaatgttc ggcttcggcc aggatcgccg ggaccaggac
     1021 cgtgctggga tggccgcgga gtgccacgtc gtcataatcc agcgcatgcg cggcggtacc
     1081 gttgatccag gcggcttccg gggcaggggc atgacggtcg ccaaacgtca gcgtggcgag
     1141 accgtcggcc ggcgcgagcg tttcggtcat gatgcggacg ctgtcttcgt tgcggccagc
     1201 gatcatggtg ccaatcgtgt cgatgaagcc catgcgggcg atgcgcgcgg cctcctccgg
     1261 caggcggttc ggcgacagct cggcgacaaa tcggccaagg gatcttgtca atgtcattgg
     1321 tcattcctcc gatcgtgctg cgcggcaggc gaccttgcgg acaagcgagg cagcaacgcg
     1381 atattctcct gatcggcagg cgcgtcaacg ggccgtgccc ggccggcttc ggtgcgaagt
     1441 ccgcagcttc gatctggcga aatatgtcac agccggttgt ggcacagtta taaattcttc
     1501 acgcgcgcct acatcggagg cgtgatgatg tcatcaccgg gacgtcctgc cggctcatga
     1561 gcaggaaatc gtccgtgatc gggaagggag gctttatgtt tcgtcaatca ttggttcttg
     1621 ctggcttcgc ggtggtcttc gctggcgcgg cttcggccgc cacgaccaaa taccatgccg
     1681 cgctgagccc aaccgcggag gttccgccca ccaagtccac cggttcgggg gaagcgaccg
     1741 cgtcgctgga tacagccacg cacgaactga cctacgatgt caccttcaag ggcttttcca
     1801 ctgatgtaac cgcggcccac atccacggtc cggccgatgc caccaagaat gccggcgtgg
     1861 tcctgccgct gggcacggcg ccaaaaagtc cgatccacgg gacggcgaaa ttgaccccag
     1921 agcaggaaca gcagctcagc gccggcatga tgtatgtgaa cgtgcacacc aaggacgatc
     1981 ccagcggagc gatccgcggg cagttggtgc aggcgaagta atcaggcgcc tcggcctcgc
     2041 cgcggcagcg gcgaggctgc acgaccagtc agcgtcagcg caccttggag ggatcatccg
     2101 ccggattggc ataggtgaac gcgaaggggc cctcggcctc gacctggatc acagcaggcg
     2161 tcttggtcca cgcgaagtgg ttcatttcgg cgggcatgcc ggcgaaccca ccggctgcga
     2221 agggctttga cgccgatcgg tccagcttgt cacccattcc ggcataaagc gtgccggaca
     2281 ggacggtcac attctcggtc gtcggatggt ggtgcgccgg gatggcgtag ttcgccggca
     2341 gcttgaggcg aatgatgaac ggacctgcct tggtcggatc gccggacagt atcgagattt
     2401 gcgcgccctt cggcaccatt gggggcgccg gcgaccattt gagagccgcc ggatttggaa
     2461 atagcgccat cggactgtca gccgccaagg cgattcccgt ggtcatgcag atcgaaaccg
     2521 ccaggctcgc gaccacgcac gaagtgaaat gacgcacgac gggctcccgt tctgattcct
     2581 gtcgtggcat gttgtcgctt cgcctgccat ccggtcaata ttttcggtcg gcctgacgca
     2641 cctatgcgac caggtgcgca tcttcggaaa agccggcaac tgaacggacg acgacgtgcg
     2701 gctgccggac tttcctttcg cgacaaagcc gtccatgcct acccgccgcc ccgcttcagg
     2761 aagcaaacca tgacccagat caga
//
LOCUS       DASWQN010000352         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2145331, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000352 DASWQN010000000
VERSION     DASWQN010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2145331"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VGC82_05765"
     CDS             complement(<1..263)
                     /locus_tag="VGC82_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEY0422814.1"
                     /translation="MSVPVVGRHRETGDRHLDALTWGLVPSFTKDLKAARKPINVRAE
                     TLSASGLFRAALGRRRCLVPASAFYEWKTTPDGKVPHAIARADG"
     gene            complement(400..672)
                     /locus_tag="VGC82_05770"
     CDS             complement(400..672)
                     /locus_tag="VGC82_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422815.1"
                     /translation="MLEQAARTRRLAIAISGHPTAPRLEQLAEELDAEIIRRVQGEPE
                     VTQDLLEQAARARRLAITIRGNQVALRLEQRAKELDAEIIRRARLT"
     gene            complement(877..1065)
                     /locus_tag="VGC82_05775"
     CDS             complement(877..1065)
                     /locus_tag="VGC82_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422816.1"
                     /translation="MNIVVILRGSNETGQRHSDIAKFLSFDTMTGFAGDILGGGHQIW
                     RAMPERQTGTPGESAPVR"
     gene            1126..>1668
                     /locus_tag="VGC82_05780"
     CDS             1126..>1668
                     /locus_tag="VGC82_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003623877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0422817.1"
                     /translation="MPAARPILIVEDDDALRLVLADQLVSDGLFQPTQAATLAEASRH
                     LAAPEARFDAIILDIALPDGDGRDFCAQIRKQGHMMPIIMLTGAAAEDDIDNGLNAGA
                     HDYILKPFRTKELLARVQAQLRRFDTSEDAVFTIGPYTFRPAAKLLIRTDTKQRIHLT
                     GKEVGVLKFLYRHANHDVTRQ"
BASE COUNT          315 a          515 c          503 g          335 t
ORIGIN      
        1 ccatccgctc gggcaatggc atgcggcact ttgccgtccg gtgtggtctt ccactcgtag
       61 aaggcgctgg ccggcaccag gcatcggcgc ctgcccagcg cggctctgaa caggcccgag
      121 gcgctgagcg tctccgcccg gacgttgatc ggtttcctgg cagccttcaa gtccttggtg
      181 aacgacggga ccagccccca ggtcagtgcg tccagatggc gatcgccggt ttcgcgatga
      241 cggcccacca ccggcacgct catggtcgga cgccatgttc caggtctgcc tcaggttggg
      301 aagcgggttc gtcgtcccga acaagcgggc gatcagttcg ggtgggagga aagaagcgta
      361 tcgaccgcac atgctgaaca gattggatcc agacaaccac tatgtgagcc gcgctcgccg
      421 aatgatctcg gcgtccagtt ctttcgcgcg ctgttccagc cgcagcgcta cttgattgcc
      481 ccggatcgtg atggcaaggc ggcgggcgcg agcggcctgc tccagtaagt cttgtgtcac
      541 ctcaggttcg ccctggaccc gccgaatgat ctcggcgtcc agttcttccg cgagctgttc
      601 cagccgtggt gctgttggat ggccactgat ggcgatggcc aggcgacggg tgcgagcggc
      661 ctgctccagc aagtcttgta tcctctcagg cgaagtcggt gacagctgtg gatatgtcat
      721 gctgtgccaa acgtcagtct gtctggagga atttcgtgca aagccggctt tgcagcgatc
      781 cacccaccag tcacaccaat agttcattgt ggaaatacaa cgtaacctca taaacaacct
      841 gagccggctg tggtgtcgcc cacgggttat gacgagctat cggacaggcg cgctttctcc
      901 gggcgtgcct gtttggcgtt ccggcattgc gcgccagatt tggtggccgc cgccgaggat
      961 atcgcccgca aacccggtca ttgtatcgaa cgatagaaat tttgcaatat cggaatgacg
     1021 ctgtcctgtt tcgttgctgc cgcgcaggat cacaacaatg ttcattgttg atatgtattt
     1081 agttacgtta cgagcatggc accgctttcg cgcgaggatc aagccatgcc ggccgccaga
     1141 cccatcctga tcgtcgagga tgacgacgcg ctacggctgg tgctggccga tcagcttgtg
     1201 tcggatggtc ttttccaacc cacccaagcg gcaacgctgg cagaggcgag ccgtcacctt
     1261 gccgcgccgg aagctcgctt cgacgcgatc atcctcgaca tcgccctgcc ggatggcgat
     1321 ggccgcgact tctgcgcgca gatccgcaag caggggcaca tgatgccgat catcatgctg
     1381 accggcgcgg cggcggagga cgacatcgac aacggcctga acgccggcgc gcacgactat
     1441 atcctcaagc cgtttcggac gaaagaactg ctggcccggg tgcaggcgca gctgcgccga
     1501 ttcgacacca gcgaggatgc ggtattcacc atcggcccct ataccttccg gccggctgcg
     1561 aagttgttga tccggacaga taccaagcag cgcatccacc tgaccggcaa ggaagtggga
     1621 gtcctcaaat tcctttaccg ccatgctaac cacgacgtca cacgtcag
//
LOCUS       DASWQN010000353         2903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_297969, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000353 DASWQN010000000
VERSION     DASWQN010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297969"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VGC82_05785"
     CDS             <1..283
                     /locus_tag="VGC82_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclohexanone monooxygenase"
                     /protein_id="HEY0422818.1"
                     /translation="EQHVDWIADCLAYLRAQDAVCIEPTAEAEEAWVAHVNEVARRTL
                     FPQANSWYMGANVPGKPRVFMPYVGGFAGYKRKCDAVAANGYEGFRLEK"
     gene            complement(347..1204)
                     /locus_tag="VGC82_05790"
     CDS             complement(347..1204)
                     /locus_tag="VGC82_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0422819.1"
                     /translation="MKWTIGDVTVTKIVELEVTGGTRFLLPQATPEEILPIAWLQPHF
                     ADENGRLRMSIHSFIVETPDRRMIVDTCLGNDKQQRRIPHWNERDGPFLADLAAAGFP
                     AESIDTVLCTHLHVDHVGWNTRLVNGKWVPTFPRARYLMGRAEYEHWSTTQDRPDMAP
                     ILADSVTPIVEAGLATMVETNERICEEISLIPTLGHTPGHVSVMIQSQGEQAMITGDF
                     MHHPCQIAYPEWSTLADSDPDRGIETRRRMFKRLAGTPVLIIGTHFAGATAGRLVVDG
                     DGYRLDPLA"
     gene            1405..2010
                     /locus_tag="VGC82_05795"
     CDS             1405..2010
                     /locus_tag="VGC82_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEY0422820.1"
                     /translation="MALHIDYYVSLNSPWTHLGAARIEAMAMANNASIRIYPVDFGAI
                     FAQSGGLPLPKRSPQRQAYRLQELARWRDHLGIPIHIQPKFFPSAEQLTSGCIIALRE
                     TAGDQPAIKLAHRVLKAVWQEEKNPGDAATLAALISEIGLDADQVMKAGSDPKWAERR
                     VADTQAALDRGVFGAPSYVIGQDIFWGQDRLEFVERRLARG"
     gene            2010..>2903
                     /locus_tag="VGC82_05800"
     CDS             2010..>2903
                     /locus_tag="VGC82_05800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY0422821.1"
                     /translation="MRVYTILPQRHWRDVGQAAAAAEAAGFDALMTVELGHDPLAPLA
                     LAALATQRVELTPSVVVAFPRSPTVLAQQAWDIQANSNGRFVLGLGSQVKGHNERRFG
                     IPWTAPAPRMRDYVQALRAIWRAWETRGRLNFDSPHYKLSLMTPDFSPEPTGLKMVPV
                     TVAAVGEAMLRVAGQVGDGVRLHPLCSRAYLEQVCMPQMMEGMRRGGRSRLNFDVHGG
                     GFVCTGPDEQTVAAEMEKARRRIGFYGSTRTYLPILALHGLEDLGGKLHRMSVEGQWD
                     RMAAEVPDDAVRIFAACGTYAE"
BASE COUNT          491 a          985 c          922 g          505 t
ORIGIN      
        1 cgagcagcac gtggactgga tcgcggactg cctggcctat ctgcgcgcgc aggacgccgt
       61 ctgcatcgag cccaccgccg aggcggagga ggcctgggtc gcgcacgtca acgaggtcgc
      121 ccgccgcacg ctgttccccc aggcgaactc ctggtacatg ggggcgaacg ttcccgggaa
      181 gccgcgcgtg ttcatgccct atgtcggcgg gttcgccggc tacaagcgga aatgcgatgc
      241 ggtggcggcg aacgggtacg aggggttcag gttggaaaag taaaagggtg gccgatcgcg
      301 aggcgcttcc tgcgccgagc gccttgccgg cgcagccgtc ggagcgttac gccaggggat
      361 cgagccggta accgtcgccg tccaccacca gccggcccgc cgtggcgccg gcaaaatgcg
      421 tcccgatgat cagcaccggc gtgcctgcca gccgtttgaa cattcgccgc cgcgtctcga
      481 tgccccgatc gggatcgctg tccgccaacg tgctccattc cggatacgcg atctgacagg
      541 ggtggtgcat gaaatcgccc gttatcatgg cttgctcccc ctgcgactgg atcatcacgc
      601 tgacatgccc gggggtatgc cccagcgtcg ggatcaacga aatttcctcg caaatccgtt
      661 cgttcgtctc caccatcgtc gctaatccgg cctcgactat cggcgttacc gagtctgcca
      721 ggatcggcgc catgtccggc cggtcctgcg tggttgacca gtgctcatac tccgcacgcc
      781 ccatcaggta ccgggcccga ggaaacgtcg gcacccactt tccattcacc agccgcgtgt
      841 tccatccgac atgatcgaca tgcaggtgcg tacacaacac cgtatcgatc gactccgccg
      901 gaaaccccgc cgccgcgagg tcggccagga acggcccgtc ccgctcgttc cagtgcggaa
      961 tgcggcgctg ctgcttgtca tttcccaggc aggtatccac gatcatgcgg cggtccggcg
     1021 tttcgacgat gaagctgtgg atgctcatcc gcagccgtcc attctcgtcg gcgaaatgcg
     1081 gttgcagcca ggcgatcggc aggatctcct ccggcgtcgc ctgaggcagc agaaaccgcg
     1141 tgccgcccgt cacttccagt tcgacgatct tcgtcacggt gacgtcgcca atggtccatt
     1201 tcatgctgcc tgcctccgtc gcgccgcatc ctacagcatg atcgcgccgg gcggaacgtc
     1261 gcggcgtgtc attcgggcgt tgaaacaaag cgataaacca tttggccgcc acagcgggct
     1321 gacgatggtc tgggccggtt cgcccggctt gtgccaatcc gcgtgctcca tcaggatcat
     1381 ggccaaagcc accggaggaa acgcatggcc ttgcacatcg actactacgt ctcgctcaac
     1441 tccccctgga ctcacctcgg cgccgctcgg atcgaggcga tggcgatggc caacaacgcc
     1501 tcgatacgaa tctaccccgt cgatttcggc gccatcttcg cccagtccgg tggcctgccg
     1561 ctgccgaagc gctcgccgca gcgccaggcg taccggctgc aggaactggc ccgctggcgc
     1621 gaccacctcg gcatcccgat ccacatccag ccgaagttct tcccgtctgc cgaacagctt
     1681 acgtccggct gcatcatcgc actgcgtgaa accgcgggcg accagccggc gatcaagctg
     1741 gcccaccgcg tgctgaaggc ggtgtggcag gaggaaaaga accccggcga cgccgccact
     1801 ttggccgcat tgatcagtga aatcggcctg gacgcagatc aggtgatgaa ggccgggagc
     1861 gacccgaaat gggccgaacg cagggtggcc gatacccagg ctgcgctgga ccgcggcgtg
     1921 tttggcgccc ctagctacgt tattggacag gacattttct ggggccagga tcggctggag
     1981 tttgtcgagc gacgcctggc gcgcggctga tgcgcgtcta taccattttg ccccagcggc
     2041 attggcggga cgtggggcag gctgccgctg ccgcggaagc cgccggcttc gatgcactga
     2101 tgacggtgga actgggtcac gacccgctgg cgccgctggc cctggcagcg ctggcgacgc
     2161 agagggtaga gctaactccg tccgtcgtcg tcgccttccc gcgcagcccg accgtcctgg
     2221 cacagcaggc ctgggacatt caggcgaatt ccaacggccg gttcgtgctt ggcctgggca
     2281 gccaggtcaa aggccacaac gaacgccgct tcggcattcc ctggaccgct ccggcacccc
     2341 gcatgcgcga ctatgtgcag gcgctgcgcg ccatctggag ggcctgggag accaggggcc
     2401 ggctgaactt cgacagcccg cactacaagc tttcgctgat gactcccgac ttctcgcccg
     2461 aacccaccgg cctgaagatg gtcccggtca ccgtcgcggc ggtcggcgag gccatgctgc
     2521 gtgtcgccgg acaggtcggc gatggcgtgc ggctgcatcc gttatgctcg cgcgcctacc
     2581 tggaacaggt ttgcatgccg cagatgatgg agggcatgcg ccgcggcgga cggtcacggt
     2641 tgaactttga cgtgcatggc ggcggcttcg tctgcaccgg ccccgacgag caaaccgtcg
     2701 ccgccgaaat ggagaaagcc cgccgccgga ttggtttcta cggttccacc cgcacctatc
     2761 tgccgatcct ggcattgcac ggtctggagg accttggcgg caagctgcac cgcatgtcgg
     2821 tcgagggcca atgggaccgg atggccgccg aagttcccga cgacgccgtc cgcatcttcg
     2881 cagcctgcgg cacctacgcc gaa
//
LOCUS       DASWQN010000354         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2408892, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000354 DASWQN010000000
VERSION     DASWQN010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2408892"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VGC82_05805"
     CDS             <1..487
                     /locus_tag="VGC82_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422822.1"
                     /translation="KPDPASAAASRRGGQIAVLISRKDSKLYVRQNFAPLFDVPVTVA
                     PDNRPLGTHVFTTQVDRNDASVLHWSVVSMPAVSGQALPRDDDEPVWRKRRLAGATET
                     KPLPLPDGPAEALDRLTVPADAMARITEALSTGGSIIVSDQGVNASETGEGTDFIVPL
                     R"
     gene            730..1674
                     /locus_tag="VGC82_05810"
     CDS             730..1674
                     /locus_tag="VGC82_05810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0422823.1"
                     /translation="MRIAAGLIFASAVSLWASGPAWSQTAATKGAATPQASANAAPAP
                     VPSMASAKAACTNPNALGISRVVEIDTTGGPGFGFEHFKQLDFLRDHEVVLTFDDGPW
                     PVNTPSVLKTLADECTTGIFFPIGKHATYYPEILKQVVAAGHTVGAHTWSHANLNDKK
                     LTEDQRKEEIEKGFSAVKWALGTSPSPFFRFPALQHPPEMVTYLGTRNVGIFSCDLDS
                     FDFKASKPQTVVDTVMKKVDKLGKGIILMHDFQKHTAEALPELLRRLKAGGYKVVKMI
                     AKAPVQTIARYDDQVLKDAKLPTVTARPVSSVVQTISE"
     gene            1687..>2053
                     /locus_tag="VGC82_05815"
     CDS             1687..>2053
                     /locus_tag="VGC82_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422824.1"
                     /translation="MPRLRIEGYVIVSAEGMLANAARLMPDELTFEGDKRFFTAALDR
                     ADLIVHGRNSHENQPNSPQRTRLIVTRRTGAIAPDPSNRQAVLWNPAGAPFETACDAA
                     GVRSGTIAIIGGPDVFGMFL"
BASE COUNT          417 a          654 c          634 g          348 t
ORIGIN      
        1 gaagccggac ccggcgagtg ccgctgcatc aaggcgcggc ggccagatcg cggtgctcat
       61 cagccgcaag gactccaaac tttatgtgcg acagaatttc gcaccgctgt tcgatgtgcc
      121 ggtgacagtc gcgccagaca atcggccgct gggcacgcac gtattcacca cgcaagtgga
      181 caggaatgac gccagtgtcc tgcactggtc cgtggtctcc atgcccgcgg tatcggggca
      241 ggccctaccg cgcgatgacg acgagccggt ctggcgcaag cgacggctcg caggtgcgac
      301 agagacgaag ccactgccgc tcccggacgg ccccgctgaa gccctcgatc gcctgacggt
      361 ccccgccgat gcaatggcgc gaattaccga agcgctgtcg acaggcggct cgatcatcgt
      421 ttccgatcag ggcgtcaatg ccagcgaaac cggtgaaggc acggatttca tcgttccgct
      481 gcgatagcgc aggcctgcct ggttccgcga tggaacttgg taaacatctg gcagacgggc
      541 tcgcccggat cgaggcatag agttccgcgg gtattaaccg gttcgagcga caagtcaggc
      601 gcggttgata aatggcgttg ccgtggcggc tccgatccta ctaggctatg atcatcgcgg
      661 gtcgcgagcg gcccaggtgg cttgaaaaaa ggcaaagccg ggcactgaca taactagagg
      721 ggaaacgaca tgcgtattgc ggcagggttg atctttgcaa gcgcggtttc actatgggca
      781 tcgggcccgg cctggtcgca gaccgcggcc accaagggcg cggcaacgcc gcaggcctcg
      841 gctaacgcag ctcccgcgcc agtcccttcc atggcctcgg ccaaagccgc ctgcaccaat
      901 cccaacgcgc tcgggatctc ccgggtcgtc gaaatcgaca ccaccggtgg accgggattt
      961 ggcttcgagc acttcaagca actcgatttc ctgcgcgatc atgaagtggt gctgacattc
     1021 gacgacggtc cgtggccggt gaacacgcct tcggtgctga aaacgctggc agatgaatgc
     1081 accaccggca ttttctttcc gatcggaaaa cacgcgacct attatccgga aatcctgaag
     1141 caggtcgtgg cggccggcca caccgtggga gcccacacct ggtcgcatgc caacctgaac
     1201 gacaagaagc tgaccgagga ccagcgcaaa gaggaaatcg aaaaaggctt cagcgcggtc
     1261 aaatgggctc tcggcacttc accgtcgccg ttcttccgct ttccggccct gcagcacccg
     1321 ccggaaatgg tgacctacct cggcacccgc aatgtcggaa ttttctcctg cgatctcgac
     1381 tcctttgact tcaaggccag caaaccccag accgtcgtcg ataccgtgat gaagaaggtc
     1441 gacaagctcg gcaagggcat catcctgatg cacgattttc agaagcacac ggcggaagcc
     1501 ctgcccgagc tgctgcgccg gctgaaggcc ggtggctaca aggtcgtcaa aatgatcgcc
     1561 aaggcgccgg tgcaaaccat cgcgcggtat gacgatcagg tcctcaagga cgccaagctg
     1621 ccgaccgtga ccgcgcggcc ggtctcgagc gtggtgcaga cgatttcgga atagacgtcg
     1681 cgcacgatgc cgcgtctgcg catcgagggc tacgtcatcg tgtcggccga gggcatgctg
     1741 gcgaacgcag ctcggctgat gccggatgaa ttgaccttcg agggcgacaa gcggttcttc
     1801 accgcggccc tcgatcgcgc cgacctgatc gtgcacgggc gcaattccca cgaaaaccag
     1861 ccgaactcgc cgcagcgaac gcggctcatc gtgacgcgcc ggaccggcgc gatcgcgccc
     1921 gatccatcca accgccaagc cgtgttgtgg aatccggcgg gagccccgtt cgaaacggcc
     1981 tgtgatgccg ccggcgtgcg atccggcacg atcgccatta tcggcggacc cgacgtgttc
     2041 ggaatgttcc tgg
//
LOCUS       DASWQN010000355         3942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_562137, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000355 DASWQN010000000
VERSION     DASWQN010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562137"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VGC82_05820"
     CDS             complement(<1..1333)
                     /locus_tag="VGC82_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0422825.1"
                     /translation="MDQNASSPTGTVAALRSPPDLGDTLKDSASTGSCAALTRRGPLP
                     TPIVFDGCFGWLHTGTGGGGDVAVMLCCGLIRDALDSHHSFRLLADRLAAAGYPTMRF
                     DYPGTGDSCDMPDGECGVAWLRSVQNGVDWLRAATGAQRIVLCGLRIGATLAALAAEH
                     RNDIAGLILLAPVLRGHSYIQQLRVEAQLHSGSSTSRSDGLDFQELRLSPKAVALIGG
                     MDLRKVELTPKQPVAVFARADSRLLSECLQAWTLRGVQTACFGFDGLEPMLRHNTQGE
                     GVPADFSEIVAWLLKAVPAQANPETVPYLPAPIRMKHPGCIETPLRFGPDDRLFGILC
                     CPDGPGADTALIIGNTGRDPHYGPARFAVGLARRLAAEGIASLRIDFAGVGDSIGHDG
                     KETLLSRLFEDDRTPDISAAIDALERQGYRRFGAHGVCAGAYHALHAALADT"
     gene            complement(1450..2202)
                     /locus_tag="VGC82_05825"
     CDS             complement(1450..2202)
                     /locus_tag="VGC82_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422826.1"
                     /translation="MNDTVALFATTADYRSIIDAVCDLRWADLDRAGISTVAWAYYYF
                     SVQFRENLQVAHGLYPDDPALDRHVAEECATNNLSPWPGVAQAGETLDHDEFMRRVLT
                     LSPIDHETRQRVEQAGRCYLSSARAEDDYAKAASIASYEDGGLERVFKAMLQCHCWDT
                     PLLEAFRHFLVKHIGFDSHPEQGHGALIRHLAPDERIVGLWSQFYTLLITAVPCLANA
                     DLPMAVGEDFGGGCKTSNALTHGTTDGTEARA"
     gene            complement(2269..3669)
                     /locus_tag="VGC82_05830"
     CDS             complement(2269..3669)
                     /locus_tag="VGC82_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY0422827.1"
                     /translation="MSESVLFPSTKTRLMIDHALTDLLAASRDRVNAGSVVPSFDAAT
                     FRDQLRTFDFENPLPIDQTLAWTVAQMETGLVHVAHPRYFGLFNPTPTFPAQCADRIA
                     ATFNPQLATSTTSPAAVEIEAHVIQAIARRTGLPPEAGGHFTTGGAEANYTALLLALT
                     RACPDFGSKGTRAFRGQPTFYVSRDSHLAWIKIAHQAGIGRSAVRLIATDGAGAMDAR
                     ALSAAIQEDRKSGHEPVMVVATAGTTNAGIIDPLHACADLTDQTGIWFHVDAAWGGAL
                     VASDKYRLMLSGIERADSITIDAHKWFAVTMGCGMFITRWRSLLFDAFYVATGYMPSG
                     NHDDPYTNSMQWSRRFLGLRLFLSLAVAGWSGYAEHVERTIALANELARKVAMLGDWR
                     VLNGHTGVICLEPPSHYRQDVRTIVKRVVASGKVWASVAEFEGRDVIRSCLTSGETTR
                     DDVTELVHCLVSCCDH"
     gene            complement(3666..>3942)
                     /locus_tag="VGC82_05835"
     CDS             complement(3666..>3942)
                     /locus_tag="VGC82_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422828.1"
                     /translation="YRRDVMRFIAHERESYPAIPHGYFSEAIIRSMESRRLDALRNRD
                     ADRSPRLRILDSAEVRCPWKPMAMQLYRNWIRLLAVEQARAAVQVAL"
BASE COUNT          833 a         1205 c         1159 g          745 t
ORIGIN      
        1 ttgtgtcggc caatgccgcg tgcaaggcat ggtaggcacc agcgcacacc ccatgagcgc
       61 caaatcgccg atacccctgt cgttccaacg cgtcgatcgc ggcactgatg tcaggggttc
      121 ggtcatcctc gaaaagccgg ctcagaagcg tttcttttcc gtcatggccg atgctgtcgc
      181 cgacgccagc gaaatcgatg cgtagggagg cgatgccttc ggctgccaaa cggcgggcca
      241 atccaacggc gaaccgagcg ggaccgtaat gcggatcacg cccggtattc ccgatgatca
      301 gcgccgtgtc ggcaccaggg ccatctggac agcacaaaat tccgaacaaa cgatcgtcag
      361 gaccgaagcg cagcggggtc tcgatgcacc caggatgctt catccgtata ggtgccggga
      421 gatacggcac ggtttccgga ttggcttggg ccggaacagc tttcagtaac cacgccacga
      481 tctctgagaa atcggccggc acgccttcgc cctgcgtgtt gtgacggagc atcggttcca
      541 gcccatcgaa gccgaaacat gccgtttgca ccccgcgcag ggtccacgcc tggaggcatt
      601 ccgagagaag ccttgaatcg gcccgcgcaa agacggcgac aggctgtttg ggcgtcagct
      661 ccactttccg caggtccatc ccgccgatca acgcgacggc tttggggctg agccgcaatt
      721 cctggaagtc gagaccatcg gatcggctcg tgctggagcc actgtgcagc tgtgcttcta
      781 cgcgcagttg ttggatatag gagtgtcccc gcagaaccgg cgccaaaagt atgagacccg
      841 cgatatcatt ccggtgttcc gcggcaagcg ctgcgagagt cgcgccaata cggaggccgc
      901 atagcacgat acgctgagca cccgtggcag cgcgcaacca gtcgacgcca ttctgaacgc
      961 tccgcagcca cgcgacgccg cattcgccgt cgggcatgtc gcaggaatcg ccggttcccg
     1021 ggtaatcgaa ccgcatggtc gggtagcctg ccgccgcaag cctgtcagcc agcaacctga
     1081 acgaatgatg agaatcgaga gcatcccgga tcagcccgca gcaaagcatg acagcgacgt
     1141 cgccaccacc gccggttccg gtgtgcagcc aaccgaagca accatcaaag acgataggcg
     1201 tgggcaatgg tccgcgacgc gtcaaggcgg cgcaactccc agtgctggcg ctatccttca
     1261 acgtgtcacc gaggtccggg ggtgaccgta atgcagcaac ggtgcctgtg ggcgatgagg
     1321 cattctgatc catgacctgc acttcagtaa cgcagataac ggagaggaaa ccgtagcgtg
     1381 atactccaaa ttgccatcga tatcgttgac ctacctagga ccatccagtc aaagattttc
     1441 gaatcacgtt caagcgcgcg cttccgttcc gtcggtcgta ccgtgggtca gggcgttgct
     1501 tgttttgcat ccaccgccga agtcttcacc caccgccatg ggcagatcag cattcgctag
     1561 acagggcact gcggtgatga gtagcgtata gaattgactc cagagtccaa ctattcgttc
     1621 atcgggtgcg agatgccgga taagtgcgcc gtggccttgc tccgggtgac tgtcgaagcc
     1681 gatatgcttc accaagaagt gccggaacgc ttccagcagt ggcgtatccc agcaatgaca
     1741 ttgcagcatt gctttgaaaa cacgttccag gccaccatcc tcgtagctgg cgatgctggc
     1801 ggccttggcg taatcgtcct cggcacgggc actcgacagg tagcagcgtc cggcttgctc
     1861 tacacgctgc cgcgtctcat gatcgatcgg cgaaagcgtc aaaacccgcc tcatgaattc
     1921 atcatgatcg agcgtttcgc ccgcctgtgc cacccccggc cacggcgaca aattattagt
     1981 cgcacactct tctgccacgt gacggtccag agccggatcg tccggatata atccgtgcgc
     2041 gacctgaagg ttctcacgga actggaccga gaaataatag taagcccagg ctacggtcga
     2101 aatgccagcg cggtcgaggt cggcccaacg aagatcacac acggcgtcga tgatcgagcg
     2161 gtaatccgcc gtcgtggcaa atagggcgac tgtgtcgttc aagtcaaagc ctttcctgtg
     2221 atgctagggg gccaaatcaa cgctgggtga atgaatcagc ttcctccact agtgatcgca
     2281 gcacgacacc aggcagtgga cgagttccgt tacatcgtcc cgcgtcgttt cgccacttgt
     2341 cagacatgac ctgatgacgt cgcgtccctc gaattccgcg accgaagccc agactttgcc
     2401 ggaagcgacg acccgcttga cgatcgtccg gacatcctgg cggtagtgcg acggtggctc
     2461 cagacagatg acacctgtat gaccgtttag tacacgccag tcgccaagca tcgccacttt
     2521 tcttgccagt tcatttgcca gcgcgatagt acgctccaca tgctcagcat acccgctcca
     2581 gcccgccacg gccaatgaca agaacaggcg cagtcccaga aaccgccggg accactgcat
     2641 tgaattcgtg tagggatcat catgattgcc cgaaggcatg tagccggtcg ccacatagaa
     2701 ggcatcaaac aacagagagc gccatcgggt gatgaacatt ccgcagccca tggtcacggc
     2761 gaaccattta tgggcgtcga tcgtgatgga gtccgcacgt tcgatgcctg aaagcatcaa
     2821 gcggtatttg tccgatgcca ccaacgcgcc gccccaggcc gcatccacat gaaaccatat
     2881 cccggtttgg tccgtaaggt ccgcgcaggc gtgcagcggg tcgatgatac cggcgttggt
     2941 cgttcctgcc gttgccacga ccataacagg ctcgtggccg cttttccgat cctcctggat
     3001 cgctgcggac agtgcgcggg cgtccatcgc tccggctcca tctgtcgcga taagacggac
     3061 cgcggagcgc ccgatcccgg cctggtgtgc gatcttgatc catgcgaggt gactgtcgcg
     3121 cgatacgtag aaggtcggct gaccccggaa cgctcgggtt cccttggagc cgaaatccgg
     3181 acaggctctg gtaagggcca gcaacaatgc ggtgtaattc gcttccgctc cgcccgttgt
     3241 gaaatggcct ccagcctcag gcggcagacc ggttcggcgg gcaatcgcct ggatcacgtg
     3301 cgcttcgatt tcgaccgcgg cgggcgaagt ggtcgatgtc gcaagctgcg gattgaacgt
     3361 cgccgcgatc cggtccgcgc actgcgccgg aaaggtcggg gtcggattga aaaggccaaa
     3421 atatcgtggg tgggccacat gaaccaaccc ggtttccatc tgggcgacgg tccaggcaag
     3481 cgtctgatcg atgggaagcg ggttctcgaa atcaaacgtg cgaagctgat ctcgaaatgt
     3541 cgcagcgtcg aaggacggga cgacactgcc ggcattgacg cggtctctgg aagcagcaag
     3601 taagtccgtc agcgcgtggt ctatcatcag gcgcgttttg gtcgacggga acaggacgct
     3661 ttcgctcata atgcgacctg caccgcggcg cgggcttgtt caacggcaag aaggcggatc
     3721 caattacggt aaagctgcat ggccatcggc ttccacgggc accgcacttc cgccgaatcg
     3781 aggatccgta gcctcggcga ccgatccgca tcgcggttcc gcagcgcgtc gagacgacgc
     3841 gactccatcg accggattat cgcttcgctg aaatagccat gtggtatggc aggatagctt
     3901 tcccgctcat gcgcgataaa gcgcatcacg tcgcgccgat at
//
LOCUS       DASWQN010000356         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1354108, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000356 DASWQN010000000
VERSION     DASWQN010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354108"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VGC82_05840"
     CDS             complement(<1..1097)
                     /locus_tag="VGC82_05840"
                     /inference="COORDINATES: protein motif:HMM:NF009472.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HEY0422829.1"
                     /translation="MADDADVIVVGGGLAGLVAATEIADAGKHVIVVEQEGEQSLGGQ
                     AFWSFGGLFLVDSPEQRRLGIKDSYELALQDWMGSAGFDRDEDAWPKRWAEAYVAFAA
                     GEKRGWLRAMGHRIFPIVGWAERGGYDAMGHGNSVPRFHVSWGTGPGVVEPFERRARE
                     AQTEGLLTFKFRHRVDALSISNGSVDGVSGAILEPDAVARGKSSSREVVGDFSFRAQA
                     VIVAAGGIGGNHDLVRQNWPKRLGDAPKNMISGVPEHVDGRMMGITEAAGARLINRDR
                     MWHYVEGIRNWSPIWPRHGIRILPGPSSLWFDATGSRLPSPLFPGSDTLGQLQYIMST
                     GYDYSWFVLTQSIIKKEVALSGSEQNPDLTG"
     gene            1391..1849
                     /locus_tag="VGC82_05845"
     CDS             1391..1849
                     /locus_tag="VGC82_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422830.1"
                     /translation="MKLLTGVLAAVLLLAGVGASHAVVRIAEDRGGRIGTYVDKYQDL
                     RTSGETVIIDGLCASACTIVLGAVAHDKICVTSHANLGFHAAWDFGANGRAVTNPEAT
                     QMLYSMYPSQVRRWIAERGGLNRHMIFLRGKQLMSMYRPCYMDAQASAPH"
BASE COUNT          339 a          659 c          627 g          366 t
ORIGIN      
        1 ttgccggtaa ggtccggatt ctgctccgag cccgacagcg cgacttcctt cttgatgatg
       61 ctctgggtca gcacgaacca ggaataatcg tagccggtgc tcatgatgta ttgcagctgg
      121 ccgagcgtgt cggagcccgg aaacagcggc gagggcaagc ggctgccggt cgcatcgaac
      181 cacagggagg aggggccggg caggatgcgg atgccgtggc gcggccagat cggcgaccag
      241 ttccggatgc cctcgacata gtgccacatc cggtcgcggt tgatcaggcg tgcacccgcg
      301 gcctcggtga tgcccatcat ccgtccgtcg acatgttcgg gcacgccgga gatcatgttc
      361 ttgggcgcat cgcccagccg cttcggccag ttctgccgca ccagatcgtg gttgccgccg
      421 atgccgcctg cggcgacgat cacggcctgg gcgcgaaagc tgaagtcgcc gacgacctcg
      481 cgcgagctgc tttttccccg cgccactgcg tcgggctcga ggatcgcgcc gctgacgccg
      541 tcgacgctgc cgttgctgat gctgagcgcg tcgacgcggt gacgaaactt gaaggtcagg
      601 aggccttcgg tctgcgcctc gcgcgcccgc cgctcgaacg gctcgaccac gccgggaccg
      661 gtgccccagc tgacatgaaa ccgcggcacc gaattaccat ggcccatcgc atcgtagccg
      721 ccgcgctcgg cccagccgac gatcggaaag atgcgatggc ccattgcgcg cagccagccg
      781 cgtttctcgc cggcggcgaa cgcgacatag gcctcggccc agcgtttggg ccatgcgtct
      841 tcatcgcggt cgaaacccgc cgatcccatc cagtcctgca gcgcgagttc gtaactgtct
      901 ttgatgccca gccggcgctg ttcgggactg tccaccagaa acaatccgcc gaacgaccag
      961 aacgcctgac cgccgaggct ttgttcgccc tcctgctcga caacaatgac gtgtttgcca
     1021 gcatcggcga tttccgtcgc ggccacgagg cctgccagcc cgccgccgac cacgatgaca
     1081 tcagcatcat cagccattgt atcatcccct gctcggaacc gattgaagcc cgctgcacca
     1141 aacatggtca acggctaatg gccggttcga actcctcccc gtcgaattac gggcttatcg
     1201 ccggattatc gaactgttcc cgcgcgggac tcggcgtgcg gtgcagcgcg cctgcaacac
     1261 ttgatttgaa tttggtttga gcgacctcgg gtggctggac aaaatcgcgg tcccgcaaca
     1321 ggctggcgac atcttgcatc actcatgtcg atcagattcg aatcggcgga ttttaaattg
     1381 gggctgggac atgaagcttc tgacgggtgt gctcgcggcg gttctgctgc tggcgggggt
     1441 aggggcgagt cacgcggtgg ttcggatcgc cgaggaccgc gggggccgga tcggcaccta
     1501 cgtcgataag taccaggacc tgcgcacttc cggcgaaacc gtgatcatcg atggcctctg
     1561 cgcctccgcc tgcaccatcg ttctcggcgc ggttgcccac gacaagatct gcgtcacctc
     1621 gcacgccaat ctgggcttcc acgcggcctg ggatttcggc gccaacggcc gggccgtcac
     1681 caacccggaa gccacccaga tgctatactc gatgtatccg tcgcaggtgc ggcgctggat
     1741 cgcggagcgc ggcgggttga accggcacat gattttcctg cgcggcaagc agctgatgag
     1801 catgtaccga ccctgctata tggacgccca ggcctcggcg ccgcactaag ccattgcttt
     1861 cgttcagcac gacgtgatgg gccggccctg cgggtcgctt gcccggcggt gcgactggtt
     1921 ctatctggag gtgagggctc aaccggcgcg ccggcctgac ctgtccaccg ggataccagc
     1981 cgtatggccc a
//
LOCUS       DASWQN010000357         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_166586, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000357 DASWQN010000000
VERSION     DASWQN010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166586"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VGC82_05850"
     CDS             complement(<1..982)
                     /locus_tag="VGC82_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0422831.1"
                     /translation="MLGLMQDWPLLCHRIIEHAAAIHGNQEIVTRSVEGPIHRTNYAK
                     IRDRALKVSQRLDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTVNPRLFPEQ
                     IAWIINHAQDRIVMTDLTFVPILEKIADKLPSVERYVVLTDTAHMPRTTLQNAVAYED
                     WIAEADGDFVWKTFDENTAAAMCYTSGTTGDPKGVLYSHRSNVLHALMANNGDSLGAN
                     AADTMLPVVPLFHANSWGIAFSAPSMGTKLVFPGPKLDGASVFELLDTEKVTHTAGVP
                     TVWLMLLNHMAANKLKLPYLRNVVCGGSAMPRSMIKAFVDMGVKVRHAWGM"
     gene            1104..2153
                     /locus_tag="VGC82_05855"
     CDS             1104..2153
                     /locus_tag="VGC82_05855"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEY0422832.1"
                     /translation="MVTVAMVLLMLCAHAAPAAARKSGGYGPWRDVVDEGRGRPRPAA
                     ALTAVPLPKPRPAEAPAAEPERPAGADHAPAAADKPDQQAAAPRPPSACRLALSEEIA
                     IAPSIPDIQGPGGCGGEDLVRLEAVVLPDHHRVSVRPAAILRCTMASAIADWIRTDMA
                     PLASSLGTVITDLDNFDSFECRGRNRVVGAKLSEHGHANALDVRALNLANGQSIALTD
                     RTTPREQRESVLRSVCARFSTVLGPGSDWYHEDHIHLDLSERRNNFKICQWDVWDPLP
                     RIAPMLPAERPDEAPPREGAATSDAKPDGTRPEAAQSDAAHSEAAEPDAGEAEPPPVQ
                     QSKPLTKKRRQIRRF"
BASE COUNT          389 a          736 c          756 g          396 t
ORIGIN      
        1 tcatgcccca ggcgtgacgc accttcacgc ccatgtcgac gaacgccttt atcatggaac
       61 ggggcatcgc cgaacctccg cagaccacgt tccgcaggta gggcagcttg agcttgttgg
      121 cggccatgtg gttgagcagc atcagccata ccgtcggaac gccggcggtg tgcgtcacct
      181 tctcggtgtc cagcaattcg aacaccgagg cgccatcgag tttgggaccg gggaagacca
      241 gcttggttcc catcgacggt gccgagaacg cgatgcccca gctattggca tggaacagcg
      301 gaaccaccgg cagcattgta tcggcggcgt tggcgcccag agaatcaccg ttgttggcca
      361 tcagcgcgtg cagcacgttg gagcgatgcg aatacaaaac gcccttggga tcgcccgtcg
      421 tgccggaggt gtagcacatg gctgccgcgg tattttcgtc gaacgtcttc cagacgaagt
      481 ctccgtcggc ttccgcgatc cagtcttcat aagccacggc gttctgcagc gtggtccggg
      541 gcatatgggc cgtgtcggtg agcacgacgt agcgttccac gctcggcagc ttgtcggcga
      601 ttttctccag gatcggcacg aaggtgagat cggtcatcac gatgcggtcc tgcgcatggt
      661 tgatgatcca ggcgatctgc tcggggaaca gccgcggatt cacggtatgg cagatcgcgc
      721 cgatgcccat gatgccgtac caggcttcga gatggcgcca ggtgttccag gcgatggtgg
      781 cgacgcggtc gccgagcttg atgccgtcgc gatcgaggcg ttgcgagact ttcagtgcgc
      841 ggtcacgaat tttggcgtaa ttggtccgat ggatggggcc ttcgaccgaa cgggttacga
      901 tctcctggtt gccatggatc gcggccgcgt gctcgatgat ccggtgacac agcaaaggcc
      961 agtcttgcat caatccaagc atacggacgt tcctccaaga tgtgttatgc cttgccgcgg
     1021 ctcgcggaat ttccatgaac tttagcgcgg agaaagcacg ccggaaatgg ccttgtggcg
     1081 gtccggcctg cggcggtggg ccaatggtta ccgtggccat ggtgctgctg atgctgtgcg
     1141 cgcacgccgc gcccgcggcg gcgcggaagt ccggcggcta cggtccctgg cgcgacgtgg
     1201 ttgacgaagg caggggaagg ccgcggcccg ccgccgcact caccgccgtg ccgctgccga
     1261 agccgcggcc agccgaagcg ccggcggccg agccggagcg gcctgccggg gcggatcacg
     1321 cgcccgccgc ggctgacaag ccggaccagc aggcggcggc gccgcggccg ccctcggcct
     1381 gccggctggc gctgagtgag gagatcgcca ttgctcccag cattcccgac atccagggtc
     1441 ccggcggatg cggcggcgag gatttggtgc ggcttgaggc cgtggtcctg ccggaccatc
     1501 atcgggtttc ggtcaggcca gccgcgatcc tccgttgcac catggcctcg gcgatcgcgg
     1561 actggattcg caccgacatg gcgccgctgg cttccagcct cggcaccgtc atcaccgatc
     1621 tcgacaattt cgattcgttc gaatgccgcg ggcgcaaccg cgtggtcggc gccaagctgt
     1681 cggagcacgg ccacgccaac gcgctcgacg tccgcgccct gaacctcgcc aatggccagt
     1741 cgatcgcgtt gaccgatcgc actacaccgc gcgaacagcg cgagagcgtg ctgcgatcgg
     1801 tgtgtgcgcg attctcgacc gtgctggggc caggctcgga ctggtaccac gaagaccaca
     1861 ttcatctcga cctgagcgag cgacgcaaca atttcaagat ctgccagtgg gacgtatggg
     1921 atccgctgcc gcggattgcg ccgatgctgc cggccgagcg gccggacgaa gcgccgccgc
     1981 gcgagggcgc cgccacgtct gatgccaagc ctgacgggac caggcccgag gccgcccaat
     2041 ccgatgccgc gcattccgaa gctgccgagc cggacgccgg ggaggccgag cccccgccgg
     2101 ttcagcaaag caagccgctc acaaaaaagc gccggcaaat ccggcgcttt tgaatctctc
     2161 cgaccgattt tcgcgatcgg ctattgcatc agcgagccgc cgaagctgcc gccttgcagc
     2221 gccatctgcg aattgtgcgg cgagtcgccg tgcttgggct ccagcaccac cacgatg
//
LOCUS       DASWQN010000358         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2013899, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000358 DASWQN010000000
VERSION     DASWQN010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013899"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VGC82_05860"
     CDS             <1..200
                     /locus_tag="VGC82_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422833.1"
                     /translation="HPQGNQRRSYQQGGDGYQGNDEGQTQAEPEDPDQRPPGLAEQRA
                     PGMGEQPEARDIPIAAAPPEG"
     gene            complement(336..1067)
                     /gene="nth"
                     /locus_tag="VGC82_05865"
     CDS             complement(336..1067)
                     /gene="nth"
                     /locus_tag="VGC82_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY0422834.1"
                     /translation="MTKAEVRAFIEALATHNPAPETELSFTDPFTLLVAVVLSAQATD
                     LSVNRATATLFKVAPTPKAMIELGVDGVASHIRSIGLWQTKSRNVVQLALALEEQHGG
                     QVPHDRAALEALPGVGRKTANVVLNVAFGESTMAVDTHIFRLGNRTGLAPGKTPREVE
                     DGLLKRLPPERLRDSHHWLILHGRYVCKARRPECWHCVAASWCRYPDKTLAPDKPRPP
                     EMTDRSAPAGAASSVVGARSAKRSS"
     gene            complement(1097..1936)
                     /locus_tag="VGC82_05870"
     CDS             complement(1097..1936)
                     /locus_tag="VGC82_05870"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0422835.1"
                     /translation="MPTATALHPLFAARVDGIDITRGFDAHWIREALDRWPVLVFPNQ
                     TITDAAQIAFSETFGPLETTRAGANGAGGKLIVLTNIGSDGRIAPPSDKQVLNNRANQ
                     IWHHDSSFKAVPARASILSAREIPSTGGDTAFCSTRAAWQAMPETLKSTARDRIAIHD
                     FGWSRARIDPALITQTERGDHPPVRRAMVATENRNGPALYLGQHARSVVGLNETDGRT
                     VIDTLMAFATNPDFVYSHRWRQGDVVVWDNRAVMHRATPFASASQRRTMVRTTASCPA
                     SVH"
     gene            complement(1952..2713)
                     /locus_tag="VGC82_05875"
     CDS             complement(1952..2713)
                     /locus_tag="VGC82_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY0422836.1"
                     /translation="MLLTAEDPAPVRVLRETSRSDIFLTADHAGRAIPRTLGQLGVSD
                     VDMQRHIAWDIGIAGVTERLSELLDATAVLQTYSRLVIDCNRDPSWPSAMPEISEHTT
                     IPGNLNLTAAAKQARIAAIFAPYHDRIAALLDARAHRRTVLVAMHSFTPSFKGESRAM
                     HVGMLYNKDARLAAILLDLLRQEGGLVIGDNAPYAVTEDSDYGVPTHGEKRGLAHVEV
                     EIRQDLIATVDGQQAWADRFARLLTKADAMLKSGQ"
     gene            2759..3067
                     /locus_tag="VGC82_05880"
     CDS             2759..3067
                     /locus_tag="VGC82_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase"
                     /protein_id="HEY0422837.1"
                     /translation="MDKEAKRAHHDLGGASKFMCEPVNIEPHGLTEFDREVDALRQML
                     AAKRLMSVDELRRGIEAIPEAEYHRLSYYRRWIRSITDNLLRHGVITEAELRAALEPE
                     "
     gene            3067..3345
                     /locus_tag="VGC82_05885"
     CDS             3067..3345
                     /locus_tag="VGC82_05885"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEY0422838.1"
                     /translation="MFSAGETVRVKDQWPEATGPVHIRTPHYIRGVVGEVVRHLGDFP
                     NPEDLAFARPAALRPLYHVRFRKGEIWPEAKPDDELLIEIYGHWLERP"
     gene            3342..>3691
                     /locus_tag="VGC82_05890"
     CDS             3342..>3691
                     /locus_tag="VGC82_05890"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEY0422839.1"
                     /translation="MNILETEGERLLTAVRALLEQKGIVSTAEVMERMAVTDAASPGQ
                     GARMVARAWVDPAYRTMMLRDGAKAAEELGIPMRGMPPLGVLENTAEVHNLIVCTLCS
                     CYPRAVLGYPPFWY"
BASE COUNT          635 a         1132 c         1273 g          651 t
ORIGIN      
        1 agcacccgca gggcaaccag cgccgtagct accagcaggg tggtgatggc tatcagggca
       61 acgacgaggg ccaaacccag gccgagcccg aggatcccga tcagcgtccg cccggactgg
      121 ccgaacagcg cgcgccaggg atgggcgaac agccggaggc ccgggacatt cctatcgccg
      181 cagctccgcc ggaaggttga tcttgctgtc gccgaaattc gtttcggccc cactacgctt
      241 cgctgatcga cttggcgagc ccggtcggtt gggcaaccct gaccggctgg gcaaccttga
      301 cccgctgggc aaccctgacc ggttgggcga aataatcagg aagatcgttt agccgaacgc
      361 gcgccgacca cggatgaggc ggcgcccgcc ggagcgcttc ggtccgtcat ttcgggtggc
      421 cgcggtttgt cgggtgccag tgtcttgtcg gggtatcggc accaggacgc ggcgacgcag
      481 tgccagcatt ccggccgccg cgccttgcag acgtagcggc cgtgcagaat aagccaatga
      541 tgcgaatccc gcagccgctc cggcggcagc cgcttcagca gtccgtcctc gacttcgcgc
      601 ggggttttcc cgggtgccag gccggtgcgg ttgccaaggc gaaaaatgtg cgtatccacg
      661 gccatcgtgc tctcgccgaa cgccacgttc agcacgacgt tcgccgtctt gcgcccgacg
      721 cccggcagcg cctccagcgc cgcgcggtcg tggggcacct gaccgccgtg ctgttcctcc
      781 agcgcaagcg caagctgcac cacgttgcgc gacttggtct gccacagccc gatcgagcga
      841 atatgagagg cgacgccgtc cacacccagc tcgatcatgg ctttcggggt aggggcgacc
      901 ttgaacaggg ttgcggtcgc gcggttcacc gacaggtcgg ttgcctgggc ggacagcacc
      961 acggcgacca gcagcgtgaa cggatcggtg aagctgagtt cagtttccgg tgctgggttg
     1021 tgcgtcgcca gggcctcgat gaacgcccgc acctcggcct tcgtcatcag cttggttttg
     1081 cgcgccggcg ccgccatcaa tggacggatg ctgggcacga ggccgtcgta cggaccatgg
     1141 tgcggcgctg cgatgcactg gcgaacggcg tggcgcggtg catgacggcg cggttgtccc
     1201 agaccacgac atcgccttgc cgccagcggt gcgaataaac gaagtccgga ttggtcgcga
     1261 acgccatcaa cgtgtcgatc acggttcgcc cgtcggtttc gttcaacccc acgacggacc
     1321 tggcgtgctg accgaggtac agcgcgggac cattgcggtt ctcggtagcg accatcgcac
     1381 ggcggacggg gggatgatcg ccgcgctcgg tttgggtaat cagcgccgga tcgatgcggg
     1441 cgcgcgacca gccgaaatca tggatcgcga tgcggtcgcg cgcggtgctt ttcagggtct
     1501 ccggcatggc ctgccaggct gcacgggtgg agcaaaacgc cgtgtcgccg ccggtggagg
     1561 gaatttcccg cgccgaaaga atggaagcac gcgccggcac tgctttgaac gaactgtcat
     1621 ggtgccagat ctggttggcg cgattgttca gcacctgctt gtccgatggc ggcgcgatcc
     1681 gcccgtccga tccgatattg gtcagcacga tcagcttgcc gcccgccccg ttggcgcccg
     1741 ccctcgtggt ctccagcgga ccgaatgttt cgctgaacgc gatctgggcc gcatcggtaa
     1801 tcgtctggtt cgggaacacc agcacgggcc atcggtccag cgcctcgcga atccagtggg
     1861 cgtcgaagcc gcgcgtgata tcgatgccat ccacgcgggc ggcgaacagc ggatgcagag
     1921 cggtggctgt cggcatgggc atatccgaat gctactggcc ggacttaagc atcgcatcgg
     1981 cctttgtcag aagccgggcg aagcggtcgg cccaggcttg ttgaccgtcc acggtagcga
     2041 tcagatcctg gcgtatctcg acttcgacat gggccagtcc gcgcttctcg ccgtgggtcg
     2101 gcacgccata gtcgctgtct tcggtaactg cgtatggcgc attgtcgcca atcaccaggc
     2161 cgccttcctg gcgaagcaga tccagcagga ttgccgccag cctggcgtcc ttgttgtaga
     2221 gcatcccgac atgcatggcg cggctctcgc ctttgaagga tggcgtgaag ctgtgcatcg
     2281 cgaccagcac cgtgcggcgg tgcgcgcggg catccagcag agcggcaatg cggtcgtggt
     2341 agggggcgaa aatggcagcg atccgcgcct gtttcgccgc tgccgtcagg ttcaggttgc
     2401 cggggatcgt agtgtgctcg ctgatctccg gcatggccga gggccaggac gggtcgcggt
     2461 tgcagtcgat caccaggcgg gaataagtct gcagcactgc cgtggcgtcc agcagttcgg
     2521 acaagcgttc cgtgaccccg gcaatcccaa tgtcccaggc gatgtggcgc tgcatgtcca
     2581 cgtcggatac gcccaactga ccgagcgtcc tggggatggc gcggccggcg tggtcggcgg
     2641 tgaggaaaat gtccgatcgg ctggtttcgc gcagcacgcg gacgggggca gggtcttcgg
     2701 cggtcaggag catcgggatt cctgttgcgg cgcatacaca atgcgctaac gtccggggat
     2761 ggacaaggaa gccaaacggg cacaccacga cctgggtggt gcctcgaaat ttatgtgcga
     2821 gccggtcaat atcgagccgc atggcctaac cgaattcgat cgggaagtcg atgcgctgcg
     2881 ccagatgctg gcggcgaagc ggctgatgtc ggtggatgaa ctccgccgtg gcatcgaagc
     2941 gatccccgag gccgaatatc accgcttgag ctactacagg cgctggatac gctccatcac
     3001 cgacaacctg ttgcggcacg gcgtgatcac cgaagccgag ttgcgcgctg ccctggagcc
     3061 ggagtgatgt tctcggcggg cgaaaccgtg cgggtcaagg atcaatggcc cgaggcgacc
     3121 ggaccggtgc atattcgcac gccgcattac attcgtggcg tggtgggcga ggtggtccgc
     3181 catctgggcg atttccccaa tcccgaggat ctggcattcg ctcggccggc ggcgctacgg
     3241 ccgctgtacc atgtgcggtt ccgcaagggc gagatctggc ccgaggcgaa accggacgat
     3301 gaattgctga tcgaaatcta cggacactgg ctggagcggc catgaacatc cttgaaaccg
     3361 agggcgaacg cttgctgacg gcggtgcgtg cgctgctgga gcaaaagggc atcgtttcca
     3421 cagccgaagt gatggaacgg atggcggtca ccgacgcggc cagcccgggg cagggggctc
     3481 ggatggttgc ccgtgcctgg gtcgatccgg cgtatcggac gatgatgcta cgggacggtg
     3541 cgaaggcggc cgaggagctc gggatcccga tgcgcgggat gcctccgctg ggggtgctgg
     3601 agaatacggc ggaggtgcat aacctgatcg tgtgcacgct gtgcagttgc tacccaaggg
     3661 ccgtgctggg gtatccgcca ttctggtaca a
//
LOCUS       DASWQN010000359         2946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2277953, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000359 DASWQN010000000
VERSION     DASWQN010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2277953"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..891
                     /locus_tag="VGC82_05895"
                     /pseudo
     CDS             60..891
                     /locus_tag="VGC82_05895"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
     gene            888..1757
                     /locus_tag="VGC82_05900"
     CDS             888..1757
                     /locus_tag="VGC82_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0422840.1"
                     /translation="MKLHLLREPLLQFLVLGAALFGLFGMLDRKNEAVAPAEIVISVG
                     RVATLADGFARTWRRPPTAQELQGLVDNYIRDEVFYREGRAAGLDRDDVVIRRRVRQK
                     MEFLAEDMAAVEPSEDQLAEYLESHPEQFRTQERLTFHQVFLSAARRGSAMENDSKQL
                     ANVLTSASAVEIGAEFGDPFLLGGESPSMSQSDIVQAFGEDFAKQISGVEQGRWQGPI
                     PSSFGLHFVFVDKRTPGNLPPLTAVRQAVQKEWLNERRLEAEQALYKKLSQRYKIVIE
                     SSSAMDARSEAAK"
     gene            1895..2776
                     /locus_tag="VGC82_05905"
     CDS             1895..2776
                     /locus_tag="VGC82_05905"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEY0422841.1"
                     /translation="MGDDLRLAIYLHWPEGAHDVAPPRSAFNGGAYTERRTIRREGGL
                     AGQVVSIEGLYVTSTDVLVRIESLGGAIQTERVSPSKTTFVVQAAPGAWEVVATYLRL
                     GIEHILFGFDHLLFVLSLVILVRDWRRVALTVTAFTVAHSFTLAAATLGFVTVPGPPI
                     EAAIALSIVLIAAEIVNARRGRGSLTVRRPWPVAFSFGLLHGFGFAGALAEVGLPHHA
                     IPIALLCFNLGVECGQLLFVAVVLTVGWLLRWVASLRFEPALLQRPIDRLDVAAAYAI
                     GAVAAYWLIVRTTAFFV"
BASE COUNT          578 a          865 c          911 g          592 t
ORIGIN      
        1 aaacgcagga aatgttggaa ttcgagtatg tccggtcggg tctcaaaaat ggtctcaaga
       61 tggaagtcga gctcagggtt aatccctaca agttcggcct gatcggcagc accgatgcgc
      121 ataccggact tgcggccgtt gaggaggata acttcttcgg caagacttcc tcctccgaac
      181 cgtgcaacgc atcccttcgt caagacggac aaggccgtca tcatgggctg ggagcagacg
      241 gcatccggct atgccgcagt ttgggcgagc gagaacacgc gcgaggcgat ctttgacgcc
      301 atgaagcgtc gcgagacata tgccacaaca ggcccgcgta tggtcgtccg cttcttcggc
      361 gggtgggatt tcgagcccaa tgacgcgaac aaccgcctgc cggctagaat cggctacacg
      421 aagggcgtcc ctatgggcgg cgatttgagc agcgcgccga gcggcaaggc gccgagcttc
      481 ctcgtcgccg cactgaaaga tccgattggt gcgaacttcg accgtatcca gatcatcaaa
      541 ggctggctcg gcaaggacgg gcagacgcag gagcgtatct atgatgtcgc tgtctccggc
      601 ggccgccaga tcggcgccga tggccggagc aggacatccg tgggcaatac ggttgacatc
      661 gagaatgcga cctggaccaa caccattggt gcgggcgaga tgatcacggt ctggaaagat
      721 ccggagttcg atccggcttt gcgcgcgttc tactacgtgt gcgtgatcga aatcccgaca
      781 ccgcgctgga ccgcgtatga tgcgaagtat tacggcatca agatgcccag cgaagtcccg
      841 atgacgacga ccgagcgcgc ctacacgtca ccgatctggt acactccttg aagctgcatc
      901 tgctgaggga gccactgctg cagttcctcg tactgggggc ggcgctgttc ggcctctttg
      961 gcatgctcga caggaagaat gaggcggtgg cgccggccga gattgtcatc tcggtcgggc
     1021 gcgtcgccac cctcgcggac ggcttcgccc gcacgtggcg gcgtccgccg actgcgcagg
     1081 aattgcaggg cctcgttgac aactacattc gcgacgaggt gttctaccgc gaaggcaggg
     1141 ccgcgggcct ggatcgagac gatgtcgtta ttcgccgccg cgtgcggcag aagatggaat
     1201 ttctcgctga ggacatggca gccgtggagc cgagcgagga ccagctcgcc gaatatctgg
     1261 aatctcatcc cgagcaattc agaacccagg aacgcctgac ttttcaccag gtcttcctga
     1321 gcgcggcacg gcgcggcagc gcaatggaga acgactccaa acaactcgca aacgtgctca
     1381 cgagcgccag tgcagtcgaa atcggggccg agttcggcga tccgttccta ctgggcggag
     1441 aatctccttc catgtcccaa agcgacatcg tgcaggcatt tggtgaagac ttcgccaaac
     1501 agatctccgg cgtcgagcag ggccggtggc agggccccat accgtcaagt tttggattgc
     1561 acttcgtctt cgtcgacaaa cgaacgcccg gaaacctgcc gccgctcact gctgtccggc
     1621 aagctgtgca gaaagaatgg cttaatgagc gccggctcga agctgagcag gcactctata
     1681 aaaagttgag ccaacgctac aagatcgtaa tcgagtcgtc gtccgccatg gacgcgcgat
     1741 ccgaagccgc gaaatgattc gcttcgcaat actccttggc gttgtcctcg ccttgcgggg
     1801 gcatccagca caggcggatg aaattcggcc aggatatctg gagctgcgcc agaccgcatc
     1861 cgacacttac agcacgctat tcaggatccc ggccttgggt gacgatctgc ggctcgcgat
     1921 ctatctccat tggcccgaag gtgcacacga cgtggcgccg ccgcgatcgg cgttcaatgg
     1981 cggtgcttac accgagcgcc gcacgatccg acgggaaggt gggcttgccg gacaggtggt
     2041 ctcaatcgag ggcctgtacg taacgtccac cgacgtattg gtgcgtatcg agagccttgg
     2101 cggggccata cagaccgagc gggtatcgcc gagcaagacg acattcgtcg tacaggccgc
     2161 acctggcgcc tgggaggtcg tcgcaacata tctccgcctg ggcatcgagc atatcctgtt
     2221 cggctttgat catcttctat tcgtcctttc attggtgatc ttggtgcggg attggcgtcg
     2281 cgtcgccctt accgttacgg cgttcaccgt tgcccacagc ttcacactgg ccgcggcgac
     2341 gcttggcttc gtgactgtac cggggccgcc catagaagcg gctatcgcgc tcagcattgt
     2401 cctcattgcc gcagagatcg tgaatgcaag acgcggacgg ggaagcctga ctgtgcgtcg
     2461 gccgtggcct gtagctttca gcttcgggct cttgcacggc tttgggtttg ccggcgcctt
     2521 ggcggaggtg ggtttgccgc atcacgcaat ccccatcgca ctcctatgct tcaatctcgg
     2581 ggtggaatgt gggcaactgc tctttgtggc tgtggtccta acagtcggct ggcttttacg
     2641 ctgggtggca tcgcttcgct tcgagcctgc cctgctccaa cggcccattg atcgactcga
     2701 cgtggccgcg gcctacgcga ttggcgctgt tgccgcttat tggctgatcg tacggacaac
     2761 cgcctttttc gtgtaaggtc tgcggaggtg tacggcgccg gtgcaaacag tagacgagcg
     2821 tgcacaagcc cgaaagcggg atgcgatgtc gctgccagca cgttaatacg aatattttca
     2881 attgtgcaac ggctgctgag acattcctgg gccaggccat gatcctggtc agattagcgc
     2941 gacgca
//
LOCUS       DASWQN010000360         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1090064, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000360 DASWQN010000000
VERSION     DASWQN010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090064"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /gene="ccsA"
                     /locus_tag="VGC82_05910"
     CDS             <1..473
                     /gene="ccsA"
                     /locus_tag="VGC82_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY0422842.1"
                     /translation="IGPVGAGFTAVCLATGSLWGKPTWGAWWVWDARLTSVFVLFLLY
                     LGHIALVRAFDDPTRGYRAGAILALVGVINLPVIKFSVDWWNTLHQPASIGLTGAPTI
                     AASMLWPLLFSAVGFTLLFAAITIARTRAAVLERRIRALLLAKATLARGQEASP"
     gene            470..616
                     /locus_tag="VGC82_05915"
     CDS             470..616
                     /locus_tag="VGC82_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422843.1"
                     /translation="MTHLPYIAAAYLIAIGLPLFFSVEALLRVRSARRRLAAIDPRGD
                     RGQA"
     gene            613..1104
                     /gene="ccmE"
                     /locus_tag="VGC82_05920"
     CDS             613..1104
                     /gene="ccmE"
                     /locus_tag="VGC82_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY0422844.1"
                     /translation="MTRKKRRLMVLLTCGIGLGSAAALTLSAFSDNLVFFVSPSDLVK
                     SNSSGRQMRLGGLVEQGTVEKSASGLAGARFKVTDGNASVMVTYKGILPDLFREGQGV
                     VVLGVEQPDGVFRASEVLAKHDETYMPKEVAEALKKSGRWNPATGSPPPASTWNGPAK
                     PSG"
     gene            1106..2335
                     /locus_tag="VGC82_05925"
     CDS             1106..2335
                     /locus_tag="VGC82_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HEY0422845.1"
                     /translation="MNEALSVQVCIIGAGPVGGTLACRLAGAGISVALIDQNTLPPME
                     NPAFDGRAYAIAAGSQTLLEQAGLWRVLDVPPNPIRDIRVSDGRPGQPPSRLHLHFSH
                     HEAGDDPRPFGFMVEARSLRTALNARFPTQDQLHLFAPATADVERSDHGATIRLQDGP
                     TIHCRLVIAAEGRNSPLRQQAGIPTTYIPYHQTAIVCAVAHEQPHHDLALEHFLPAGP
                     FAALPMGASADAEPGGAANVSAIVWTERTKDAGAFLGLDDRRFAREASRRLGSHLGGI
                     RAVGRRWSYPLSAMIAHRYVATRLALAGDAAHGIHPIAGQGLNLGFRDAIALTDLIIQ
                     ASQAGEDVGSEALLQRYQRVRQADNLLMFAMTDRIDRLFSTDLRVVRMVRDAGIDAVN
                     RMRPIKRVFMRRAMGLA"
     gene            complement(2382..>3263)
                     /locus_tag="VGC82_05930"
     CDS             complement(2382..>3263)
                     /locus_tag="VGC82_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY0422846.1"
                     /translation="ALIKHEAIGLNFIDVYFRTGLYKAPSLPLIIGQEGAGTVIATGV
                     NVSNVVVGDRVAYAGSLGGYATERVIPADRLVKLPDNISFQTGAAMMLQGMTAQYLLH
                     RTHTVKPGETIVVHAAAGGVGLILCQWAKHIGATVIGVVSNEEKAALARAHGAAHTVI
                     GHAGLAAEVKRITGGAMVPVVYDSIGKDTFNASIDCLAPLGLMASYGNASGPVPPVEL
                     GMLAAKGSLFLTRPTLATYTAKRKDLERVAADLFDVVGSGAVKIQVNQTYSLKDVGAA
                     HTALETRKTTGSTVLLP"
BASE COUNT          554 a         1036 c         1037 g          636 t
ORIGIN      
        1 aaatcggacc cgtgggcgcc ggatttaccg cggtctgttt ggcaaccggc agcctgtggg
       61 ggaagccgac ctggggcgcc tggtgggtct gggacgcgcg cctgacctcg gtatttgtcc
      121 tttttcttct gtacctcggc cacatcgcgc tggtgcgggc gttcgacgat ccgacgcggg
      181 gctaccgggc tggagcaatc ctcgcgctcg tgggtgtgat caatctgccg gtcataaaat
      241 tcagcgtcga ttggtggaac acacttcatc aaccggcgtc catcggcttg acaggcgctc
      301 cgaccatcgc cgccagcatg ctgtggccac tgctgttctc ggccgtcggg ttcacccttc
      361 tgttcgccgc aatcaccatt gcccgcaccc gggccgccgt gctggagcgg cgcattcgcg
      421 cgttgctgtt ggcaaaagcc accctggccc gtgggcagga agcgtcgcca tgacccatct
      481 tccctatatc gcggctgcct acctcatcgc gatcgggctg ccgcttttct tctccgtcga
      541 agccctgctc cgggtccggt cggcgcgccg gcggctggcc gctatcgatc cgcgcggcga
      601 ccggggtcag gcatgacgcg caaaaagcgg cggctcatgg ttttgctgac ctgcggcatc
      661 gggcttgggt ctgctgcggc actcacttta tcggcgttca gcgacaatct ggtgttcttc
      721 gtctcaccgt ccgatttggt gaaatccaat tccagcggac ggcaaatgcg ccttggcggc
      781 ttggtggaac agggcaccgt cgagaagtcc gcttccgggc tggccggtgc ccggttcaag
      841 gtcacggacg gcaacgccag cgtaatggtg acgtataaag gcattcttcc ggacctgttc
      901 cgcgagggac agggcgtggt cgtcctcggg gtggagcaac cggacggggt gtttcgtgcg
      961 tcggaggtgt tggccaagca cgatgaaacc tacatgccga aggaggtcgc cgaagcgctg
     1021 aagaagtccg gccgctggaa ccccgcgaca ggatcgccgc cgccagcttc cacctggaac
     1081 gggccggcaa agccatcagg ttgatatgaa cgaagctctt tctgtgcagg tctgtatcat
     1141 cggtgccggc ccggtcggcg gcacgctggc ctgccgtctc gcgggcgctg ggatcagcgt
     1201 ggcgctgatc gaccaaaata cgcttccacc gatggaaaac ccggctttcg acggccgggc
     1261 gtatgcgatc gcggcggggt cgcagacatt gctggagcaa gcggggcttt ggcgtgtcct
     1321 tgacgtgcca cccaacccca ttcgggacat ccgcgtcagc gacggccgcc cagggcaacc
     1381 gccctcaagg ctgcacctgc atttcagtca ccacgaagcc ggcgacgacc cacgtccgtt
     1441 cggcttcatg gtagaagcga ggtcgttgcg cacagccttg aacgcccgat ttccgacgca
     1501 ggatcagttg cacttgttcg cgcccgcgac cgccgacgtg gagcgatcgg atcacggtgc
     1561 cacgatccgc cttcaggacg ggccaaccat ccattgccgg cttgttattg ccgcggaggg
     1621 tcgaaattct ccgctgcggc aacaggcggg gattccgacc acctacatcc cgtatcatca
     1681 gacagcgatc gtttgcgcgg tggcgcatga gcaaccccac catgacctgg ctttggaaca
     1741 tttcctgccg gccggcccgt tcgccgcact tccgatgggt gcaagcgcgg atgcggagcc
     1801 cggtggggcg gcgaatgtct cggccatcgt atggaccgag cggaccaagg acgccggggc
     1861 gtttctcggg ctggacgata gacggttcgc gcgggaggcc agccgccggc tgggctcgca
     1921 tctaggaggg attcgtgcgg ttggccgtcg gtggagctat ccgctgtcgg cgatgatcgc
     1981 gcatcgctac gtcgctacgc ggttggccct ggccggagac gccgcccacg ggatccaccc
     2041 gatagcgggc cagggactga accttggttt tcgcgatgcg atcgcgttga cggacctgat
     2101 catccaggct tctcaagcag gcgaagatgt gggttcggag gctttgctgc agcgctatca
     2161 acgcgtgcgg caggcggaca acctgctgat gttcgcgatg acggatcgaa tcgacaggtt
     2221 gttcagcacc gacttgcggg tcgtacgcat ggtccgggac gcagggatcg atgcggtcaa
     2281 tcgcatgcgg ccaataaaac gcgtattcat gcgcagggca atgggcttgg cctgaaacgg
     2341 cgcggcttcc gctccggcat tccagccggc ccagtcgtaa tctatggaag cagaacggtg
     2401 ctgcccgtcg tcttcctcgt ctccaatgca gtgtgcgccg cgccgacatc tttcaggctg
     2461 taggtctgat tcacctggat tttcacggcg ccgctgccca ccacatcgaa taggtcggcc
     2521 gccacgcgct ccaggtcttt ccgcttcgcc gtataggtcg caagcgtcgg ccgcgtcagg
     2581 aacaagctgc ccttggccgc gagcatgcca agctccactg ggggtactgg gcccgacgca
     2641 ttcccgtagc tcgccatcaa gcctaaggga gccaagcaat cgatgctggc gttgaaagtg
     2701 tcctttccga tgctgtcgta aaccaccggc accattgctc cgccggtaat acgcttcact
     2761 tcggcagcca aaccggcatg cccgatcacg gtatgagccg cgccatgggc tcgggctaat
     2821 gctgccttct cctcgttcga gacgacaccg atcaccgtgg ctccgatatg tttcgcccac
     2881 tggcaaagga tcaggccgac cccgcccgcg gcggcatgca cgacgatcgt ctcaccgggc
     2941 ttcaccgtat gggtccggtg cagcaggtat tgcgcggtca tcccctggag catcatggct
     3001 gcgccagttt ggaagctgat gttgtccggc agcttcacca ggcggtccgc cggaatcacg
     3061 cgttcggtcg catagccgcc cagcgaaccg gcataggcaa cccggtcgcc gacgacgacg
     3121 ttcgaaacgt tcacacctgt cgcgatgacc gtaccggcgc cttcctggcc gatgatcaac
     3181 ggcaggctgg gggctttgta caagcccgtg cgaaaataca cgtcgatgaa attgaggccg
     3241 atcgcttcgt gcttgatcag cgc
//
LOCUS       DASWQN010000361         4750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1354679, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000361 DASWQN010000000
VERSION     DASWQN010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354679"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VGC82_05935"
     CDS             <1..538
                     /locus_tag="VGC82_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0422847.1"
                     /translation="PRHAIRQGIYLVPEDRKRAGLILEMPVVENITLASLLAHAKLLL
                     VNRGSEMAASKTQRARLSIRTPGDLTRVATLSGGNQQKVVLAKWLSMRPRVVIFDEPT
                     RGIDVGAKNEIYTLMRELADSGVSILMISSDMEEVIGVSDRIAVMHEGRISGILQRSQ
                     FSESNVLHLAIGQRSRAA"
     gene            538..1551
                     /locus_tag="VGC82_05940"
     CDS             538..1551
                     /locus_tag="VGC82_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0422848.1"
                     /translation="MLPKKELGLLLLLVVISAITGALNPQFLSGVNLLDMANLIGLFG
                     IFSIGEGLTIITGGIDLSVGSMFSLLGVVFIDLVANRDVGWPLALVAVLAAGLALGAI
                     QGFLITRLRMQPFIVTLCGLLIYRGAARYYTDDSTMGFGYGADADTLSWLASGRSFGV
                     PHSFMFLIVVALVLGVVLHRSVFGRYLFAVGKNEEAARYSGISTHAVIAGAYVLSGLL
                     AGISTIFLVFYTNSVSPSTFGNFYELYAIAAAVLGGCSLRGGEGSILGIVLGTALLQV
                     LQNLVNILGIPSSLNFAVMGTVILLGVLADQQLQKRREAAIAATAQRGRPASPAVRPG
                     RAT"
     gene            complement(1571..2149)
                     /locus_tag="VGC82_05945"
     CDS             complement(1571..2149)
                     /locus_tag="VGC82_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422849.1"
                     /translation="MSTADPNELILTGENPFIRLSDKDGSPNTTNASFWRIITCPAGP
                     GHVLYLHSELTENRWRIYSDNIAMARWLQSTVQGMLNAETADRAIPVTEARFSKSGDP
                     RYFWTEQVSARNEEISLTWYDIGEPLLIHTQPNADPGRPYGVCTVLIPALGARLSRNG
                     VEAAGQPWPREREGRPFSTCALAFSESWTKAR"
     gene            2400..4046
                     /locus_tag="VGC82_05950"
     CDS             2400..4046
                     /locus_tag="VGC82_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEY0422850.1"
                     /translation="MSVVARFELVLLLLAVIIVLELTARRLRLPPAAAFVLGGMALAL
                     IPGTPDVELDPDLALVLFLPPLLLASAFFTVWRDFRANLRIILQLAVGAVAFTTLTVG
                     IIAHLVAPALPWAACFALGAIVSPPDAVAAKAVLQGLRLPRRVVVLLEGESLVNDASG
                     LVLFRLAVAASLTGTFSATSAALSFVTLSVGGVLAGLAFGGIASVVLKRLREPTLGIL
                     GSVLMAWASYIGAEAIAVSGVLSTVTCGLVMGWRQHAILTATTRTQARAVWKVLVFVL
                     ESLIFILIGLSLRGILSRLGMSGPGMHGPGMHDLGMHNLDDGPSALFPAMGAIVGAVV
                     LARFVWIFPATYLPRWFIPALRRRDPIPPLAVPLVMSWAGMRGVVSLAAALALPEQFP
                     GRDFILATTFAVILATVLVQGATLAPLILALRVGRFTQAQTSMLTEATARARVAAAQL
                     SVVAARSAMPDGSQRHPRLLEQYDYRARAATHYSEAEESLQGHRDAHFSVILDAVAAG
                     RAELLRLHRSGEIHDSVLHALEEELDLDEMRARQAIGNGA"
     gene            complement(4058..4687)
                     /locus_tag="VGC82_05955"
     CDS             complement(4058..4687)
                     /locus_tag="VGC82_05955"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEY0422851.1"
                     /translation="MANTLNVGMDHIELAIVLAVDGSASVTYEEFGLIAGGMAAALRD
                     PAVAAGLVGHSILSLLLWSGPEQQDIITGWSAIETAADLDDFAAGVDNMPRTVAAGTT
                     AIGEALLASLTLLANIPVIPRRNIVNVIGDGRSNDGIAPGPVRDRMAAADITINGLCI
                     LHEEPDLLTFYTNEVIGGPNSFAVTCRGYQDFTEAMRQKLTREINGPIV"
BASE COUNT          774 a         1455 c         1538 g          983 t
ORIGIN      
        1 gccgcgccat gccattcgcc agggcattta cctcgtaccg gaagaccgca agcgcgccgg
       61 cctgatcctg gaaatgcctg tcgtggagaa catcacgctc gccagcctgc tggctcacgc
      121 gaagctgctg ctggtcaacc gaggctccga gatggcggca tcaaaaacac agcgggctcg
      181 tttgtcgatc aggacgccag gagacctgac ccgggtcgcg acactgtccg gcggcaacca
      241 gcagaaggtc gtgctggcca aatggctgtc gatgcggcct cgggtcgtca ttttcgatga
      301 gccgacacgc ggcatcgacg tcggtgccaa gaacgaaatt tacacgctga tgcgcgaact
      361 tgccgatagc ggcgtgtcga tactgatgat ctcgtccgac atggaggagg tgatcggcgt
      421 gtcggaccgc attgccgtga tgcacgaggg acgaatcagc ggcattctgc agcgttccca
      481 gttcagcgaa tccaacgtgc tccacctcgc tatcggtcaa agatcgcggg ccgcgtgatg
      541 ctgccgaaga aagagcttgg cctgctgctg ttgctggtgg tgatcagcgc catcaccggg
      601 gcgctcaatc cgcagttcct gtcgggcgtg aacctgctgg acatggccaa cctgattggg
      661 ctgttcggga ttttctcgat cggcgagggc ctgaccatca tcaccggcgg catcgacttg
      721 tccgtgggtt cgatgttctc gctgttgggc gtggtgttca tcgatctggt cgccaacagg
      781 gacgtcggct ggccgctggc gctggtcgcg gtactggcgg ccggacttgc gcttggcgcg
      841 atccagggct tccttatcac ccgcctgcgc atgcagccgt tcatcgtaac gctttgcggt
      901 ttgctgatct atcgcggcgc cgcgcgctac tacaccgacg actcgacgat gggctttggt
      961 tacggcgccg acgcggacac cctgagctgg ctggcgtccg gccgcagctt cggcgtgccc
     1021 catagcttca tgttcctgat cgtcgtggcg ctggtcctgg gcgtcgtgct gcatcggtcg
     1081 gtgttcggcc gctacctttt cgcggtcggc aagaacgagg aagcggctcg ctattccggc
     1141 atcagcacgc acgcggtcat cgcgggtgcc tatgtcctga gcggcctgct cgccggcatt
     1201 tccaccatct tcctggtgtt ttacacgaac tctgtttcac cctcgacgtt cgggaatttc
     1261 tacgagctgt atgccatcgc tgccgcggtt ctgggcggtt gcagcctgcg cggcggcgag
     1321 ggttcaatcc tgggcatcgt tcttggcacg gcgctgcttc aggtgttgca gaacctggtg
     1381 aacattctgg gcatcccaag ttcgctcaat ttcgcggtga tggggacggt cattctgctg
     1441 ggcgtgctgg cggatcagca gttgcaaaag cggcgcgagg cagcgatcgc agccaccgcg
     1501 caacgcggac gccccgcgag tccggccgtc cggccagggc gtgcgacctg acaggagcgg
     1561 cggcgtgcat ctatcgcgcc ttggtccagc tttcggagaa tgccagcgca caggtgctga
     1621 agggacgccc ttcgcgctcc ctcggccaag gctgacccgc tgcctcgaca ccgttgcgcg
     1681 ataaccgagc gccgagcgct ggtatcagga cggtgcatac gccgtacggg cgcccgggat
     1741 cggcattggg ctgcgtatgg atcaacaacg gctcgccgat gtcataccag gtgagagaaa
     1801 tctcctcgtt tcgcgccgac acctgttcgg tccagaaata gcgcgggtca cccgacttgc
     1861 tgaaccgcgc ctccgttacc gggatcgccc gatcggcggt ttcggcgttg agcattccct
     1921 ggacggtcga ttgcagccaa cgcgccatcg cgatgttgtc ggagtaaatg cgccagcgat
     1981 tctccgtcag ctcgctgtgg aggtagagga catgccccgg accggccgga caggtgatga
     2041 tgcgccagaa gctggcattc gtggtgttcg gactgccgtc tttgtcgctc aagcggataa
     2101 acggattctc accggtgagg atgagttcat ttggatctgc ggtgctcatc gagatgtttc
     2161 cccctgatga cctcagcgta aggcaaggca cgcgttcgcg ccacgggcac cacaggttgt
     2221 acggcaaggc atgtcgccct ggccgaacgg aacgcgcaat ccaggtggat catgtgatcg
     2281 gactgagggc acccgttcca cgcaagccgc gaatagccgg tcaacatggg ctcctgcgcc
     2341 tggtgatggc ttgatcaggc aggggtaaca tcgctgctcg gccgtgctat ccctcgtgca
     2401 tgagtgttgt cgcccggttc gagctcgtcc ttctcctgct tgccgtcatc atcgtcctgg
     2461 aactgacagc gcggcgcctt cggttaccgc cagccgccgc gttcgtgctc ggtgggatgg
     2521 cgctggcgct gatacccgga acgccggacg tcgagttgga tccggacctt gcactggtgc
     2581 ttttcttacc gccgctgctg ctggccagtg cgtttttcac ggtatggcgg gatttccgcg
     2641 ccaacctgcg aattatcctg cagctcgcgg ttggtgccgt ggcattcacc accctgaccg
     2701 tcgggatcat tgcgcacctg gtggcgccgg cgttgccatg ggcggcgtgt tttgcccttg
     2761 gcgccatcgt ctcaccaccg gatgcggtag ccgccaaggc cgtacttcaa ggtttacggc
     2821 tgccgcggcg agtggtcgtc ctgttggagg gcgaaagcct ggtcaacgac gcctctggcc
     2881 tggtgctgtt ccgcctcgca gtcgccgcca gcctgaccgg cacgttcagc gcgacgtctg
     2941 cggcgctcag cttcgtcaca ttgtccgtag gcggcgtatt ggccggcctg gcattcggcg
     3001 gaattgcgtc cgtggtcctg aagcgcctgc gcgaaccaac gcttggcatt ctcgggagcg
     3061 tcctcatggc ttgggcgtcc tacattggtg cggaggcgat tgccgtgtcc ggcgtgctat
     3121 ccacggtaac ctgcgggttg gtcatgggat ggcgccagca cgcgattctg accgccacca
     3181 caagaaccca agcaagagcg gtttggaagg tgctggtatt cgttctggaa tcgctcattt
     3241 ttatcctgat tgggctgtcg ctccgcggga ttttgagccg cctcgggatg agcggccccg
     3301 gaatgcatgg ccccggaatg catgacctcg ggatgcacaa tctggacgac ggtccaagcg
     3361 cactgtttcc ggcgatgggg gccattgtcg gcgcggtggt cctcgcgcgg tttgtctgga
     3421 tctttccggc aacgtacttg ccgcgctggt tcataccggc gttgcgacga cgcgatccaa
     3481 taccgccttt ggcggtgccg ctggtcatga gttgggcggg catgcgtggt gtcgtcagcc
     3541 tggcggcggc actggcactg ccagagcagt ttccgggccg cgacttcatt ctggcgacga
     3601 cctttgccgt gattctcgcg acggtcctgg ttcagggtgc taccctggcg ccgttaatcc
     3661 tggcgctgcg cgtcggcaga tttacccagg cacagacatc gatgctgacg gaggcaacag
     3721 cccgagcacg cgtggcggcg gctcagcttt ctgttgttgc ggcgcgttcg gccatgcccg
     3781 acggcagtca gcgccatccc cgcctgctgg agcaatatga ctaccgcgcc cgcgccgcca
     3841 cccactacag tgaggcagag gaaagcctgc agggacatcg cgacgcccac ttctctgtga
     3901 ttctcgacgc agtcgccgcc ggacgggccg aattgctccg gttgcaccga agcggcgaga
     3961 tccatgattc ggttctgcac gccctggaag aggaactgga cctcgacgag atgcgcgctc
     4021 ggcaggccat cggtaacggt gcctgagctt agtcagacta gacgatcggt ccgttgattt
     4081 ctcgcgtcag cttctgccgc atggcctccg tgaagtcctg atatccacgg cacgtaacgg
     4141 cgaaagaatt cgggccgccg atgacctcat tggtgtagaa agtcagcaag tcgggctcct
     4201 cgtgcaggat gcacaggccg ttgatggtta tgtcggccgc tgccatgcga tcccgcacag
     4261 gcccgggagc aatcccgtcg tttgaccggc cgtcgccgat gacgttgacg atgttgcgcc
     4321 tggggatgac gggaatgttg gccagcaggg tcagcgaggc cagcagcgcc tcaccgatgg
     4381 cagtggtgcc ggccgcgact gtgcgcggca tgttgtcaac gccggccgcg aaatcgtcca
     4441 ggtcggcagc cgtctcgatc gcactccagc cagttatgat gtcttgctgt tcgggcccgg
     4501 accatagcag cagggacaaa atgctgtgtc cgaccaggcc cgccgcgacg gccgggtccc
     4561 gcaaagcagc cgccatcccg ccggcgatca aaccgaattc ttcataggtc acgctggcag
     4621 agccatcgac tgccagcact atcgcgagtt cgatgtgatc catgccgacg ttaagggtgt
     4681 ttgccaagag gcggtcaacg accggtttat gtggttgaga aagtggcgga ggctgggcga
     4741 cgccagtctg
//
LOCUS       DASWQN010000362         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2409882, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000362 DASWQN010000000
VERSION     DASWQN010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2409882"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VGC82_05960"
     CDS             <1..409
                     /locus_tag="VGC82_05960"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088636.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HEY0422852.1"
                     /translation="GIILGLLAAEMTARSGRDPGQLFDQLTGELGMPFYERIDAPATA
                     AQKNLLKALSPQKLAMKELAGEPVRAVLTAAPGNGQPFGGIKVTSDHGWFAARPSGTE
                     DVYKIYAESFRSHDHLRQIQRDAQAAIARAFTA"
     gene            complement(399..614)
                     /locus_tag="VGC82_05965"
     CDS             complement(399..614)
                     /locus_tag="VGC82_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422853.1"
                     /translation="MPESETRSTASPAPAVLSNCPQCDADLVVLRIIPGRSSEYWTLR
                     CTKCGGIHLDIVKPRSNVVQLDRALRP"
     gene            complement(642..896)
                     /locus_tag="VGC82_05970"
     CDS             complement(642..896)
                     /locus_tag="VGC82_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422854.1"
                     /translation="MRYEYDGFTIEAFEAGTALWHARIMRADLKPVVIDGVSFSALEV
                     GFAWASADAAVSDAKFQIDQLRLKRYATVGELPQGERSAA"
     gene            1055..>1691
                     /locus_tag="VGC82_05975"
     CDS             1055..>1691
                     /locus_tag="VGC82_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0422855.1"
                     /translation="MTPLMISALGLLMVATAFLSGLFGMAGGLILIGVLLALMPLPTA
                     MVLHAITQMASNGWRAFLWRSHIQWRPVAVYLIGCAVALGAWSITRYVPDKPVALLLL
                     GVTPFMARLLPPGLKPDPASVWQGTLYGSICMGLMLMTGVSGPLLDTFFLGGKFDRRG
                     IVATKATCQVASHFVKLIYFGGIIDQAATLDPVLAAVAVAASMTGTTLARRI"
BASE COUNT          279 a          532 c          576 g          304 t
ORIGIN      
        1 cggcatcatc ctggggctat tggccgccga gatgacggcg cgcagcggcc gcgaccccgg
       61 ccagctgttc gatcaactga ccggcgagct cggcatgccc ttctatgagc ggatcgacgc
      121 gccggcgacg gcggcgcaga agaacctgct gaaagcgttg tcgcctcaaa aactcgcgat
      181 gaaagagctc gccggcgagc cggttcgcgc ggttctcacc gcggcgcccg gcaacggtca
      241 accgttcggc ggcatcaagg tgacgtcgga ccatggatgg ttcgcggcgc ggccgtcggg
      301 caccgaggat gtctacaaga tctatgcgga gagttttcgc agccacgatc acctgcggca
      361 aatccagcgc gacgcccagg ccgcgatcgc gcgggcgttc acggcctgag cgcgcggtcc
      421 agttgaacga cattggagcg gggtttgacg atatcgaggt ggatgccgcc gcatttcgtg
      481 catcgcagcg tccagtattc ggacgagcgg cccgggatga tgcgcaacac cacgagatcg
      541 gcgtcgcatt gcgggcagtt ggacagcacc gcgggagcgg gcgaggcggt ggatcgggtt
      601 tcggattccg gcatgggaag cgccctttat ttcaaatcag gttaagccgc cgaccgctcg
      661 ccctggggca attccccaac ggtcgcgtat cgcttgagcc ggagctggtc gatctggaat
      721 ttcgcgtccg acaccgcggc gtcggcgctg gcccaggcaa agccgacctc gagcgccgaa
      781 aacgagacgc cgtcgatgac cacgggcttg aggtcggccc gcatgatccg ggcgtgccac
      841 agagctgttc cggcctcgaa agcctcgatt gtaaacccat catactcgta ccgcatggcg
      901 tgactccgga agttgggagc ccagccaaac atggacggcg gggaacggat gtgaactcgc
      961 tcacattcga agcggattat tgccgcgccg cgcagcttgt aagtcggttc gcgcaggcgc
     1021 tacaaccggc cagcaaaggt ttgcggggat tcgaatgacg ccgctgatga tctcggcgct
     1081 tggcctgttg atggtcgcga ccgcgttcct gtccggcctg ttcggcatgg ccggcgggct
     1141 gatcctgatc ggcgtgctgc tggccctgat gccgctgccg accgcgatgg tgctgcacgc
     1201 gatcacccag atggcgtcga acggctggcg tgcctttctg tggcgcagcc atatccagtg
     1261 gcggccggtc gcggtctacc tgatcggatg cgcggtggcg ctgggcgcat ggtcgatcac
     1321 ccgctacgtg ccggacaagc cggtcgcgct gcttctgctc ggggtgacgc cgttcatggc
     1381 gcggctgttg ccgccgggcc tgaagcccga tcccgccagc gtctggcagg gcaccctgta
     1441 cggctccatc tgcatggggc tgatgctgat gaccggcgtc tccgggccgc tgctggacac
     1501 gttctttctg ggcggaaaat tcgatcgccg cggcatcgtc gcaaccaagg ccacctgtca
     1561 ggtggccagt catttcgtga aactgatcta ttttggcggc atcatcgatc aggccgccac
     1621 gctcgatccg gtgctggcgg cggtggccgt ggcggcatcg atgaccggca ccacgctggc
     1681 gcggcgcatc c
//
LOCUS       DASWQN010000363         3293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_167121, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000363 DASWQN010000000
VERSION     DASWQN010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167121"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VGC82_05980"
     CDS             <1..461
                     /locus_tag="VGC82_05980"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HEY0422856.1"
                     /translation="WGEGSVQLIEIPTKEEIHDNVAGYWRPKNVLSAKGEYTYTYRLH
                     WGPDVPKATSLARFLRTAVGAKGEDARIFVLDVVGEKLKSADAQTIRGQVSAGKAEIR
                     NVVTQPNPATGGWRLSFELPIKEKVPVELRASLMQGNDPLSEVWVYRWTP"
     gene            536..2599
                     /gene="mdoH"
                     /locus_tag="VGC82_05985"
     CDS             536..2599
                     /gene="mdoH"
                     /locus_tag="VGC82_05985"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEY0422857.1"
                     /translation="MAPQALQTFRRGEDQRAATNSLGIRRACILVGTAVMSIAGCYLM
                     YEVLQVGGVTTLEWMVLGLFVLLFTWIAFSFMSALAGFMVLLFRIKDPLGIDPDAPLP
                     SVASKSAMLLPTYNENPYRIMARLQAMYESLGQTGSGSAFDWFVLSDTTDPSVWIAEQ
                     KCYLRLRAELGATNIFYRHRPENHARKSGNIEDWIRRFGAGYEQMIILDADSLMTGDA
                     VVRLVSAMERHPGVALIQTLPVVVNARTLFSRLQQFSGRLYGPIIAAGIAWWHGSEGN
                     YWGHNAIIRVRAFAKDAGLPELHGRKPFGGHILSHDFVEAALMRRAGWAIRMAPALGG
                     SFEECPPSLLDFAARDRRWCQGNLQHLAVLPASGLHWVSRLHLLTGIGSYLTAPLWLM
                     FLVLGILISLQAQFVRPEYFPKGFALFPKWPAQDPILAAWVFVGTMGLLILPKLLGYV
                     LLLTHTRTRRQFGGGLRVFAGVLVETFLSGLIAPVMMIFQSSAVGEILLGRDAGWQVQ
                     RRDDGKWPVRDLVRKYSAPTLFGAVMAGSAFAVSVPLLLWMAPVILGLLLSVPIAMLS
                     SRRSDPHSRMLRTPEQTAPPEVLIRANELAEARDEIVACPLLALRNDAGLLDAHLASL
                     SGERPRNRGQINPQLAIARAKIEDAESLDDALSYLTPRELFAVLNSPSMLATVLALPH
                     RQQRP"
     gene            complement(2829..>3293)
                     /locus_tag="VGC82_05990"
     CDS             complement(2829..>3293)
                     /locus_tag="VGC82_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY0422858.1"
                     /translation="FNPEALGYIRRQSPHPISSCETLLGLREFLPYFREQAMDVAIID
                     TPWNGVWQSMKVAAAAEAYEVNVAPHNFYGHLCTMMNAHFSAAVPHLRIMETDIDRLA
                     WDHELFTHVPEFEDGYLVLPDRPGWGTEPNEEALRAHPPKNPGGLLNYGQKA"
BASE COUNT          587 a          989 c         1032 g          685 t
ORIGIN      
        1 attggggcga aggctcggtt cagctgatcg agatccctac gaaagaggaa atccacgata
       61 acgtcgcggg ctattggcgg ccgaagaacg tgctctcggc caagggtgag tatacctata
      121 cgtaccgcct gcattgggga ccggacgtcc ccaaggccac atcgctggcg cggtttttgc
      181 ggacagctgt cggtgccaag ggcgaagatg caagaatatt cgttctggac gtggttggcg
      241 aaaagctgaa atcagccgat gcgcagacca tccgcggtca ggtttccgcg ggcaaggccg
      301 aaatccgcaa cgtcgtcacc cagccgaacc cggcgaccgg cggctggcgc ttgagttttg
      361 agctgccgat caaagagaag gtcccggtcg aattgcgcgc atcgctgatg cagggcaacg
      421 acccgctgtc ggaagtttgg gtctaccgat ggacaccctg acaccacggc cggacggtga
      481 aatgtcctgc ggcggttcgc ggcagcaatt cctgccgccg gcggcgccga tggaaatggc
      541 gccgcaggcg ctgcaaacgt ttaggcgcgg ggaagaccaa cgggctgcta cgaattcgct
      601 ggggatacgg cgcgcctgca ttctcgtcgg tactgcggtc atgtccattg ccggttgtta
      661 cctgatgtac gaagtccttc aggtcggcgg cgttacgacg ctcgaatgga tggtcttggg
      721 tctgttcgtg ctgctgttca cctggatcgc attctcgttc atgtcggcgc tggccgggtt
      781 catggtgctg ctgttccgca tcaaggatcc gctcggcatc gacccggacg cgccgcttcc
      841 gtcggtcgcc agcaaaagcg cgatgctgtt gccgacctac aatgaaaatc cgtatcggat
      901 catggcccgg cttcaagcga tgtatgagtc gctcggtcag accggatctg gttcggcgtt
      961 cgactggttc gtgctgagcg acaccaccga cccttcggtc tggatagcgg agcagaagtg
     1021 ctaccttcga cttcgagcag agcttggcgc caccaatatc ttctatcgcc accgccctga
     1081 aaatcacgca cgaaagtccg gaaacataga agactggatc cggcggttcg gcgccggcta
     1141 tgagcagatg atcatcctgg acgccgacag cttgatgacc ggcgacgcgg tggtccggct
     1201 cgtctccgca atggaaaggc atcccggcgt cgccctgatc cagacgctgc ccgttgtcgt
     1261 caacgccagg accctgttct cccgcttgca gcaattctca ggccggcttt acggcccgat
     1321 catcgcggcg ggcattgcct ggtggcacgg ctccgaaggc aattattggg gccacaacgc
     1381 gatcattcgc gtgcgcgctt tcgcaaagga tgcgggcctg ccggaacttc acggccgcaa
     1441 gccgttcggc ggtcacattc tgagccacga cttcgtcgaa gccgcgctga tgaggcgggc
     1501 agggtgggct atcaggatgg cgcccgcgct tggcggcagc tttgaagaat gcccgccatc
     1561 gctgctggat ttcgcagccc gcgaccgccg atggtgccag ggcaatcttc agcatctggc
     1621 agtgcttccc gcgagcggtc tgcattgggt atcgcggctc catcttctca ccgggatcgg
     1681 ctcttatctc acggcgccgc tctggctgat gtttctcgtg ctcgggatct tgatctcctt
     1741 gcaggcccaa tttgtcaggc cggaatactt tccgaaggga tttgcattgt ttccgaaatg
     1801 gcctgcccag gatccgattc tcgccgcctg ggtttttgtc ggcaccatgg gattgctgat
     1861 ccttcccaaa ctgctcggtt acgttctgct cctgacacat accagaaccc gccggcagtt
     1921 cggtggtgga ttgagggttt tcgcgggggt actggtcgag acgtttctgt caggcctgat
     1981 cgcgcctgtc atgatgatct ttcaatcgag tgcagtgggc gaaattctgc tcggacgcga
     2041 tgcaggctgg caagttcagc ggcgcgacga cggcaaatgg ccggttcgcg acctggtccg
     2101 caaatattcg gcgccgactc tgttcggcgc cgtgatggct ggcagcgcgt ttgccgtatc
     2161 ggtaccgctg ctgctctgga tggccccggt cattttggga ttgttgctct ccgttccgat
     2221 cgcgatgctc tcctcgagac ggagtgatcc ccattccaga atgttgcgta ctccggagca
     2281 aaccgctccg ccggaggtgc ttatccgtgc aaatgaactg gccgaggccc gcgatgaaat
     2341 cgtcgcctgc ccgctgctcg cgcttcgtaa tgacgctggc ctgctggacg cgcatctggc
     2401 gagcctgtcc ggggagcggc cgcgcaatcg cggccaaatc aatccgcagc tcgcgatcgc
     2461 gcgggccaaa atagaggatg cggagagcct ggatgatgcg ctgagctatc tcactccgcg
     2521 ggagctgttt gcagtcctga attcacccag catgctggcg actgtgcttg cccttcctca
     2581 tcgccagcaa cgtccttagc ctggatcggc tccgtcattc atgggccacc tcgggctggt
     2641 cgggatacga cccggcccgt cgccgtagga cgagcagata accactgatt agagacggcg
     2701 agaacccgag gccagcggac ggctcaaggc gaggccgcca cggtcgcgga ttttgccagt
     2761 tcgcggactc ccggtgcatg ctgcgaacga cgccccggtt gaattcaccc gcttgccggc
     2821 atcagttgct aggctttttg accgtagttc agcagcccac cgggattctt cggaggatgc
     2881 gcacgaagcg cctcttcgtt gggctcggtc ccccagccgg gccggtccgg caggacgagg
     2941 tagccgtcct cgaactcggg cacatgggta aacagctcgt gatcccatgc cagtcgatcg
     3001 atatcggttt ccataatccg caggtgcggc accgccgcgc tgaaatgggc gttcatcatg
     3061 gtgcagagat ggccgtagaa attatgcggc gcgacgttga cctcataggc ctcggcggcg
     3121 gcggcgacct tcatcgactg ccagacgccg ttccacggcg tgtcgatgat cgcgacgtcc
     3181 atcgcctgct cgcggaaata gggcaggaac tcgcgcaggc ccaacagcgt ctcgcatgat
     3241 gagatcgggt gaggactttg cctccggatg taaccgaggg cttcgggatt gaa
//
LOCUS       DASWQN010000364         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_299100, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000364 DASWQN010000000
VERSION     DASWQN010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299100"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1115)
                     /locus_tag="VGC82_05995"
     CDS             complement(27..1115)
                     /locus_tag="VGC82_05995"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY0422859.1"
                     /translation="MIKRSLRASLVGALPLLLAAALPVAGVSAADVKSYDSSKPSFWA
                     HPPPDWFLGDETEAQKGLAPPVGPATPTPLAEVQDNLKKIKLPKGFKIEVYASGLPEA
                     RQMAWGNNGTLFVGSFNATNVYAVTDQGGKRTVKTILKGLNMPTGIAFRDGALYVVAI
                     NKLMRYDNAEANLDKMPEPVVVYDDMPPYVAHGWKYIVTDKDGWMYLPFGPPFNIGIP
                     PTSVSQVRHVDPKTGLAEVVALGVRNSVGGDIDPRTGDYWFTENARDWISDDLPSDKL
                     NHITRIGQHFGYPYCHQGNLPDPKFARGHDCKEFTPPVLNLGPHVAPLGMKFYTGNQF
                     PTEYKNNIFIAEHGSWTATSIRVDGPNG"
BASE COUNT          304 a          442 c          455 g          316 t
ORIGIN      
        1 cgggttcttg ccgtcagggc caacaatcac ccgttcggtc cgtccaccct gatacttgtg
       61 gcggtccacg agccatgttc ggcaatgaag atgttgttct tgtactcggt gggaaactga
      121 ttcccggtgt aaaatttcat cccaagcggc gcgacgtgcg gaccgaggtt aaggactggc
      181 ggcgtaaatt ccttgcagtc atggccgcgg gcgaatttcg gatcgggcaa gttgccttga
      241 tggcaatacg ggtatccgaa gtgctgcccg attctggtga tgtggttgag cttgtcgctt
      301 ggcaggtcgt cactgatcca atcgcgggcg ttctcggtaa accaataatc gcccgtacgc
      361 ggatcgatat cgccgccgac gctgttgcgc acgcccagag cgacgacctc cgccaaaccg
      421 gtcttcggat cgacgtgcct gacctgggat acgctggtcg gtggaattcc aatgttgaac
      481 ggcggcccga acggcaaata catccaaccg tccttgtcgg taacgatgta tttccaccca
      541 tgcgccacgt acggcggcat gtcgtcgtaa accacgaccg gctcgggcat cttgtccagg
      601 ttggcttcgg cgttgtcgta gcgcatcaac ttgttgatgg cgaccacgta cagtgcgcca
      661 tcgcggaagg cgatcccggt gggcatgttg aggcccttga ggatggtttt gaccgtgcgc
      721 ttgccgccct ggtcagtgac ggcatacacg ttcgtcgcgt tgaacgagcc gacgaacagg
      781 gtaccgttgt tgccccacgc catctgccga gcctcgggca ggccactggc atagacctcg
      841 attttgaagc ctttcggcag ctttatcttt ttcagattgt cctgcacctc cgccagcggc
      901 gtgggagtcg ccggccccac gggcggtgca aggcccttct gcgcctccgt ttcgtcgccg
      961 aggaaccagt ccggaggcgg atgcgcccaa aacgaaggct tgctggagtc atagcttttg
     1021 acatcggcgg ccgaaacgcc ggcgactggc agcgccgccg caagaagcag tggtagcgcg
     1081 ccgaccaggc tcgcgcgcag tgaccgtttt atcatgacct atcctcccga tggacaatcg
     1141 agcgttgttg cttcctgaac gcgaaaagct tgcctgcacg atgtcgggaa cgtcaccccc
     1201 ggcaaccagg acatatgaac gtcggcaaca tgacgaatcg atatagaagc aaagccaacg
     1261 tgcggcaccg gtgtcgccct gtctataggt ttttgtcggg acaaggtcgc gtggaagcct
     1321 gcgatcaggc gaccaggcct gatcgttcgc cggccgctgg caaagaaacg tcagtgccaa
     1381 gggatgcacc acaaatgaga acaggacgaa agcgcgttcc gctgccgcgt gaacttcgac
     1441 gggtaccgaa atcaggacaa gttgccatgt tgctgccacg agccggttgt attcatgacc
     1501 ctgtagaaaa cccgata
//
LOCUS       DASWQN010000365         3611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1354907, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000365 DASWQN010000000
VERSION     DASWQN010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354907"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VGC82_06000"
     CDS             complement(<1..797)
                     /locus_tag="VGC82_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422860.1"
                     /translation="MSSRRQRLPDRTEQDLFPEIETRQNLAAANDRDRDFWLGALRGA
                     LAARSNQSLEIAEQALKAWPADPDLLLLAALTATAAGKPERSLALLKRYGKRYVSSKP
                     AALLTALALGAQAQHAQALVMLRADKLDNDQAALNQFVGEEGMTDWLLAHLREIRMLG
                     SLSLWKVRGKAPAKALAKVVAKKPPKKAPAASRLPRLTQVAPELPTVAGLPKLEARFD
                     MAFEIGNPDSIEIAGADPDPAPFHLRAELVRLSMFEGFDELLCLPAL"
     gene            complement(1117..2289)
                     /locus_tag="VGC82_06005"
     CDS             complement(1117..2289)
                     /locus_tag="VGC82_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY0422861.1"
                     /translation="MPVYPTRLGRYETTTAGGETVRAFIPLPLPPIPAIDLSGLQIAL
                     EQANQTVGRLDGIASVLPDPSLFLYMYIRKEALLSSQIEGTQSSLSDLLLYEVDEAPG
                     VPIDDTREVSNYVAAMNHGLQRLRDNFPLSLRLIREIHAKLLSKGRGQDKDPGEFRRS
                     QNWIGGTRPGNALFVPPPPHLVMDCLGALETFIHADAPDLPLLVKAALVHVQFESIHP
                     FLDGNGRLGRLLITFLMCERGILREPLLYLSLFFKQNRETYYELLQAVRERGEWETWL
                     EFFLRGVTDTARQGTRTAQSLLRLFNEDRARIEGLGRPAATVLRLHQFLQRRGLTTIP
                     SASRQLGLSQPTVTSAIAHLQTLGVVRETTGRQRGKIYMYGTFLDLLSEGTDPLPR"
     gene            2434..3558
                     /locus_tag="VGC82_06010"
     CDS             2434..3558
                     /locus_tag="VGC82_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEY0422862.1"
                     /translation="MLQVPPAIPGMREEEIDTPALVIDLDAFEANLDTMAALLAPTGV
                     KLRAHAKTHKSPVIAKLQMARGAVGNCVQKVSEAEVLAWGGIPDILVSNEVVGAAKLA
                     RLCALSRIAKVAVCADDAGQVGAIEAAAADAGIRMTVLVEIDVGAGRCGVQPGPDAVA
                     LATRIAGSKHLIFGGLQAYHGSAQHKRTPAERRTLIGGAVDASRRTVEQLRQQGLDCA
                     IVGGAGTGTFEIESNSGVYTEMQAGSYIFMDADYARNLNEAGMPVSTFRHALFVLSTV
                     MSQSQAGVAALDAGHKAVAVDSGLPTIWQRPDIQYTSASDEHGKLQFGSETAAPKVGE
                     KLLLVPGHCDPTVDKFDWYVGVRQGRVECLWPVAARGGLS"
BASE COUNT          674 a         1092 c         1182 g          663 t
ORIGIN      
        1 tgcagcgccg gcaggcaaag cagttcgtcg aagccctcga acatgctcag ccggacgagt
       61 tcggcgcgca aatggaacgg cgccggatcg gggtcggcgc cggcgatctc gatcgagtcg
      121 ggattgccga tctcgaaagc catgtcgaag cgcgcctcca gcttcggaag gccggcaacg
      181 gtaggcagtt ctggggcgac ttgggtgagc cgcggcagcc gggatgccgc tggggccttc
      241 ttcggtggct ttttggctac tacctttgcc agggctttcg cgggggcctt tcctcgcacc
      301 ttccacaagc tgagtgaccc gagcatccgt atctcgcgca agtgggcgag cagccagtcc
      361 gtcatccctt cttcgccaac gaactggttt aatgcggcct gatcgttgtc cagtttatct
      421 gcccgcagca taaccagcgc ctgggcgtgt tgtgcctgcg cgcccagggc aagcgctgtc
      481 agcagcgcag ccggcttgct ggaaacgtag cgcttgccgt agcgcttcaa tagcgccagc
      541 gagcgctcgg gcttccccgc cgccgtcgcc gtcagcgcag ccagcaacag caagtcgggg
      601 tcggctggcc aggccttcag ggcctgttcg gcgatctcga gcgactggtt tgaccgggca
      661 gccagagctc cccgcagcgc gccaagccag aaatcccgat ccctatcgtt cgcggcggcc
      721 aggttctgcc gtgtttcgat ctcaggaaac aggtcttgct cggtccggtc cggtagcctt
      781 tggcgtcggg aactcatggt gtcacctccg cggcgaatcg agacggtcga tggcgcatgg
      841 accatcatcc cgcagatggt cacagacgcc aacaagcttt aggttacgtg tttcaagcgg
      901 gtgtctccgg gtcttcaccg gcagcgccat gatccggcaa atcacttcgg gcgtagacgt
      961 cgctggcgca ccatagcccg gatcaatcat ctgacgcgga actccggcgc gtagggtggc
     1021 gagtcttggg cataatgcaa cacctcttgc gaaagcttca gggtgtccac gacggcgggt
     1081 catcccagtt tccgtctgca ttaagacttg ctgtcgttaa cgcggcaaag ggtcggtgcc
     1141 ttcgctgagt agatccagga aggtgccata catgtaaatc ttcccccgtt gccggccggt
     1201 ggtttcgcgg acaacgccga gtgtttgcag gtgtgctatg gcgctggtga cagtgggttg
     1261 cgacaacccg agttgccgtg aggcactggg aatcgttgtc aatccgcgtc gctgcaggaa
     1321 ttggtgcagt cgcagcacag tggcggccgg ccggccgagc ccctcgattc gagcgcgatc
     1381 ctcgttgaac aaccgcagca ggctttgcgc ggtccgggtg ccttgcctgg cggtatcggt
     1441 aacgccgcgg aggaagaatt caagccatgt ctcccactcg ccgcgctccc gtacggcttg
     1501 cagaagctcg tagtaggttt cccggttttg cttgaagaac aggctgagat agagcagggg
     1561 ttcgcgcagg attccccgtt cgcacatcaa gaaggtgatc agcaggcgcc ccaggcggcc
     1621 attgccatcc agaaaaggat ggatggattc gaattggaca tggaccaggg ccgctttcac
     1681 cagcagtggc agatccggtg cgtcggcatg gataaaagtt tccagcgcgc ccaggcagtc
     1741 catgaccagg tgcggcggcg gcggcacgaa caatgcgttg cctggccgcg ttccgccaat
     1801 ccagttctga ctgcggcgga actcccccgg gtccttgtcc tggccacggc ccttcgacag
     1861 cagttttgcg tggatctcgc gtatcagtcg aagcgacagc gggaagttgt cccgcaggcg
     1921 ctgcaatccg tggttcatcg cggcgacgta gttggaaacc tcgcgggtgt cgtcgatcgg
     1981 gacgccaggg gcttcatcca cctcgtagag caaaaggtcg gacagggagg attgcgtgcc
     2041 ctcgatctgg gaagacagca gcgcttcctt gcggatatac atataaagga acaaggatgg
     2101 atcgggcagc acggaagcga tcccgtcgag ccgtccaacc gtttgattgg cctgttcgag
     2161 ggcgatctgc aggccgctga gatcgatagc cggaatcggc ggcaacggca gtgggataaa
     2221 cgcacgaact gtttccccac cggcagtggt ggtttcgtag cggcccagac gggttgggta
     2281 caccggcatc gtgaagcctt gttaaaggat gctttaatag cagcttgaac tattaaggaa
     2341 tatggcgtaa ttttttaatt actccgactg cccctctccg actgcccctc atggccaaag
     2401 cggcaaaact atccgcatcc tggaggaacg ccgatgttgc aagtgccccc cgccatcccc
     2461 ggcatgcgtg aagaagaaat cgacacacca gctctggtca tcgacctgga cgcgttcgaa
     2521 gcgaatctcg acaccatggc ggccctcctg gcgcccaccg gggtaaagct gcgggcgcac
     2581 gccaagacgc ataagtcccc ggtcatcgcc aaactgcaga tggcccgcgg cgcggtcggc
     2641 aactgcgtgc agaaggtgag cgaagccgag gtgctggcct ggggcggcat ccccgacatc
     2701 ctggtcagca acgaagtcgt cggggccgcc aagctggcac gcctgtgcgc cttgtcgagg
     2761 atcgccaagg tcgcggtctg tgccgacgac gccggacagg tgggggcgat cgaggccgcc
     2821 gccgccgatg ccggcatccg catgacggtg ctggtggaaa tcgatgtcgg cgccggccgc
     2881 tgcggtgtgc aacccggacc ggacgcggtg gcactggcga cccgcatagc cgggtcgaag
     2941 catctgattt tcggcgggtt gcaagcctat cacggcagcg cccagcacaa gcggaccccg
     3001 gcggaacggc gcacgctgat tggcggtgca gtcgatgcct ctcgccgaac ggtggaacag
     3061 cttcgccagc agggactgga ctgtgcgatc gtcggcggcg cgggaaccgg cacgtttgag
     3121 atcgaatcga actccggcgt ttatacggaa atgcaagctg gcagctacat cttcatggac
     3181 gccgactatg cgcgcaacct gaacgaggcg ggcatgccgg tcagcacgtt ccggcacgcg
     3241 ctgttcgtgt tgtccacggt catgagccaa agccaggccg gcgtggcggc gctggatgcc
     3301 gggcacaagg cggtggcggt cgactccgga ttgcccacca tctggcagcg gccggatatc
     3361 cagtacacca gcgcatcgga tgagcatggc aagctccagt tcggcagtga aaccgccgcc
     3421 ccaaaggtgg gcgagaagct gctgctggtt cccggccact gcgatccgac agtcgataag
     3481 ttcgactggt acgtcggcgt acgccagggg cgggtggagt gcctatggcc cgtcgcggcg
     3541 cgcgggggac tgagctagtc ccgaaagccg cacggcccgc ggaacctggg gagaacatcc
     3601 gcgccagtct g
//
LOCUS       DASWQN010000366         5281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2146582, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000366 DASWQN010000000
VERSION     DASWQN010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2146582"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1312
                     /locus_tag="VGC82_06015"
     CDS             143..1312
                     /locus_tag="VGC82_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422863.1"
                     /translation="MSPSDAAWPQLISALHASGRKAALAITGGGSGAIGELLRVPGGS
                     RLLIEAQVPYDSLALAMFLGFAPAQACSSDTAIALARSARARAASLVPVGSDLVGLGV
                     TAALVSDRPRKGEHRFHIAFANFAGIAHCTGVLAKGRRDRAAEEDLVSHAIILWLSQA
                     CGVAAPSPRSLLDADEHYAEAVIDTIDAIDQLVAGEINRVTAQPDGQLMLSAPTPVML
                     LPGSFNPLHAGHMLLARVAEEIRQQPLAFEISVTNVDKPPLVGTTVRHRLAQFAWKSP
                     VELTRAPTFVEKARLFPGTTFVIGADTAERLVAPKYYGDDEVRMHAALEEIGGSGCSF
                     MVAVRIDAAGRLRRLSDIPMPPRFADLFTEIPEHRCRFDTSSSEIRARGGAAGFS"
     gene            1727..2353
                     /locus_tag="VGC82_06020"
     CDS             1727..2353
                     /locus_tag="VGC82_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY0422864.1"
                     /translation="MIVVHHLNDSRSQRILWLLEELNVPYDIKHYQRDPQTRLAPPEL
                     KAIHPLGKSPVITDAGRVIVESGAIIDYIIRHHGQGRLQPDPSTPKYDDYVQWLHFAE
                     GSPMLPLLLKLYVSRLGEAGAPLGPRIESEIANHLGYIDASLEDRDYLLGAELTGADI
                     QLSFVGEIAGARGNRMIYPNLDAWVRRFQARPAYQAALSRGGPYNYAS"
     gene            2462..3433
                     /locus_tag="VGC82_06025"
     CDS             2462..3433
                     /locus_tag="VGC82_06025"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0422865.1"
                     /translation="MAILITGAAGFVALNIVEHLLTAGRQVIGLDRIPLPRRAVRDFA
                     ALPGHLTMIGGSILSDADLSRALTIAPVEAVIHCAVITAGSQREIADPGSIIEVNIQG
                     AVATLVAAARRGVKRFVYPSSGAVYGAAAATVDLMDEDALRPAPVNLYGLTKFAAESI
                     LPRVAETRGVALTAARLGVVYGPWEYATGVRDTLSPMLQTLELAAAGAEAVLGPPWRG
                     DYTLSRDIAAGLVALADLPSTRRTTYNLATGKATTAEDWCRVLATRLPRFRWRRAAEG
                     EAWNVESHTGFDHGAMRIDNITADTPYRPWFDLADAADHLIAWRAAG"
     gene            complement(3507..4340)
                     /locus_tag="VGC82_06030"
     CDS             complement(3507..4340)
                     /locus_tag="VGC82_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0422866.1"
                     /translation="MSVLAKWGLLSQAERDAAYDNLAAVADSKVLNVAREVASSVFRT
                     TNCLHLDLRYGKMERNTWDLFPQRDPDAPCIVFIHGGYWQRNSKDQFANLISGAYARG
                     WSAALPGYTLAPNASLTEIVAEINAALDWLAAHGPAHGLNGPVVLSGWSAGGHLTAMC
                     LGHRLVKAGLAISGIYELGPVRDTNLNDKLRLTEQEIVALSPLRLPPVAKPLAIAYGT
                     DELPALVSDSRDLHALRSTAHVPGALIPIPRANHFTALHELRDPDGILTRYLPLLLPQ
                     R"
     gene            complement(4337..>5281)
                     /locus_tag="VGC82_06035"
     CDS             complement(4337..>5281)
                     /locus_tag="VGC82_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0422867.1"
                     /translation="RRASRHHLLERTASPGHHPIMTDPQKPHQRLDYQAAIDRPRLIL
                     PDGKRVAVFLVVNIERWDIDRAMPRQVLSAPQGAAVIPDLPNWAWHEYGMRVGFWRLK
                     AALDVHGIAPTLAINGMVPSAYPQVTKACRDAGWEFMVHGHHQMATHLVADQRAMIRD
                     AIAALREAGGHDPVGWLAPGLTEDLDTPDLLAEAGIKYCADWVVDDLPTTIRTAHGPM
                     LTMPYSVELNDIPMMMIQHHRAEELFDRTIAQFERLHEEAGEQGAKVMGLAVHPYISG
                     VPHRIGWFERVLAHIASRPGALFWQGRQIMDWYQKATA"
BASE COUNT          916 a         1687 c         1687 g          991 t
ORIGIN      
        1 gcgaccaaaa ggggacattc taactttgga cgatggggac attcctactt ggggctaaca
       61 cacccatgtg cgctgtttga ctttgtccaa tctgtctttc accatctgcg gggcactcct
      121 cagttttgga cacatataca ccatgagccc gtcggacgcc gcgtggccac aattgatctc
      181 cgccttgcac gcgtccggcc gcaaggctgc actcgcgatc accgggggcg gcagtggggc
      241 gatcggcgag ttgctgcgtg ttcctggtgg ttcacgtctg ctgatcgagg cgcaggttcc
      301 ctacgactcg ctggcacttg caatgtttct cggctttgca ccggcacagg cgtgcagttc
      361 cgacaccgcg atcgcgctgg cacgaagcgc acgggcccgc gcagccagtt tggtgccggt
      421 gggcagcgac ctggtgggcc tcggcgtgac ggcggcgctg gtgagcgatc gcccgcgtaa
      481 aggcgagcat cgatttcaca tcgcatttgc caattttgcc ggcatcgcgc actgtactgg
      541 ggtgctggcc aagggccggc gtgatcgcgc cgccgaagaa gacctcgttt cgcacgcgat
      601 catcttatgg ctgtcccagg cctgcggggt agccgcgccg tcgccgcgca gcctgcttga
      661 tgcggatgaa cattatgcag aggcggtcat cgacacgatc gatgccatcg atcagcttgt
      721 ggcaggtgaa atcaatcgcg ttacggccca gcccgatggc cagttgatgc tgtcggcgcc
      781 gactcccgtt atgctgttgc cgggttcttt caacccactg catgcaggac atatgttgct
      841 ggcgcgggtc gccgaagaga tcaggcagca gcccctggcg ttcgaaatct ccgtcacgaa
      901 cgtcgataag ccgccgctgg tggggacgac agtgaggcac cggctcgcgc aattcgcttg
      961 gaagtcgccg gtcgagctta caagagcgcc caccttcgtc gagaaggcgc gtttgtttcc
     1021 gggaacgacg tttgtgatcg gggcggatac ggcggagcgg ctggtcgcgc cgaaatacta
     1081 cggcgatgat gaggttcgaa tgcatgccgc gctggaggag attggcgggt ccggctgcag
     1141 cttcatggtg gcggtgcgta tcgatgccgc ggggcgcctg cgcagattga gcgatatccc
     1201 tatgccgcca cgctttgctg atctcttcac tgaaatcccc gagcatcgtt gccgctttga
     1261 cacatcttcg tcagaaattc gtgcacgggg aggtgcggct ggtttttcat agcgagcgtc
     1321 aaaacataat ccgcatcctg gctacggtcg gtgcatccca gaaaaccgcc caagtgattg
     1381 cgctatcaag tcttctgccc tgcgctggcc cccacgccat tgccgcattc aggcacagtc
     1441 atcggtggcc acgccagccc ggccatgatt ccaccatcaa ccgctgcccg gccgtggaca
     1501 tcgctggcgc ttttggcgcc ttcgcccgca ataatggttc tggagaccgc gaccgaagcg
     1561 gttatttcgg agcaggcctt tacgaacggt aagggcgcgc gtgaaatcgc caggaatggc
     1621 aacgcccgcg atccggatga agttggtgcc tccgcgttac ccgcaacctg tcgctccggg
     1681 cggccgcagg taaaaaggaa ccgccgggaa attctgggag aacctgatga tcgtcgtcca
     1741 ccatctgaac gattcccgct cgcagcgaat cctgtggctg ctggaggaac tgaatgtccc
     1801 ctacgacatc aagcactacc agcgtgaccc gcaaacccgc ctcgcgccgc cggaactgaa
     1861 ggccatccac ccgcttggta agtcgcctgt catcacggac gccggccggg tgatcgtcga
     1921 gtccggggcg attattgact atatcattcg ccatcatggc caggggcggc tacagccgga
     1981 cccctcgacg ccgaaatatg acgattacgt ccagtggctg cacttcgccg agggctcccc
     2041 catgttgccg ctgctgctca agctgtacgt gtcccggctg ggcgaagccg gcgcgccgct
     2101 tggcccgcgc atcgaaagcg aaatcgccaa ccatctgggt tacatcgatg cgtcgctgga
     2161 agatcgcgat tacctgctgg gcgccgaact gaccggtgcc gacatccagt tgagttttgt
     2221 gggcgagatc gcgggcgcgc gcgggaaccg catgatttat cccaatctgg acgcctgggt
     2281 acgtcgcttc caggcacgcc cggcctatca ggccgcgttg tccaggggtg ggccctacaa
     2341 ctacgcgtcc tgaggctgcg ggctgcgagt cgggtaaagg tccgcggggt cccttggacg
     2401 gacagcccgg cgcggaccgc ccgccttgcg cgagcaacgc cctggcgctg taatggaacc
     2461 catggcgata ctgataaccg gtgcggccgg cttcgtagca ttgaacatcg tcgagcattt
     2521 gctgacagca ggccgccagg tcattggcct cgaccgaatc ccgctgccca gacgcgccgt
     2581 tcgcgacttc gccgcgctgc caggacatct gacgatgatc gggggcagca tcctgtcgga
     2641 tgccgacctg tcgcgggccc tgacgatagc gcccgtcgag gcggtcatcc actgcgcggt
     2701 catcaccgcc ggttcgcagc gggaaatcgc tgatcctggc tccatcatcg aggtcaacat
     2761 ccagggtgcg gtcgccacgc tcgtcgccgc tgcccgacgc ggcgtaaaac gctttgtcta
     2821 tccaagctcc ggcgcggttt atggcgccgc ggcggcaacg gtggatctga tggatgaaga
     2881 tgccctgcgg ccggcacccg tgaatcttta tggcctgacc aaattcgcgg ctgaatccat
     2941 tcttccccgc gtcgccgaaa cccgaggtgt ggcactaacc gccgcgcgtc ttggcgtcgt
     3001 ttacggcccg tgggaatacg cgactggcgt gcgggatacg ctatcgccga tgctgcaaac
     3061 gctggaactc gccgccgccg gggccgaggc cgtgctcggc ccaccgtggc gcggcgacta
     3121 cacgctgtcc agggacatcg ccgccggcct tgttgccctg gccgatctgc catcgacacg
     3181 caggacgacc tacaatttgg caactggcaa agccaccacg gccgaagatt ggtgtcgggt
     3241 gttggcgaca cgattgccgc ggttccgctg gcgccgggca gcggagggtg aggcgtggaa
     3301 tgtcgaaagc cacaccggct tcgaccatgg tgccatgcgg atcgacaaca tcaccgccga
     3361 cacgccgtat cggccttggt tcgacctggc ggacgccgcc gaccacctga tcgcctggag
     3421 ggcagcgggc tgatgcctac gccagggaac gggctggaag cctcgtgtct cactgaccgc
     3481 ggttttcaag cgtaaccgca gcgccgctag cgttgtggaa gcaacaacgg cagataccgc
     3541 gtcaggatac catccggatc ccgcagctcg tgcagggcag tgaaatgatt cgctcgcggt
     3601 atcggaatca acgcccctgg cacatgggcc gtcgaacgta gcgcgtgcag gtcgcgactg
     3661 tccgacacca gggccgggag ttcatcggtg ccgtaagcga ttgccagagg cttggcgacc
     3721 ggcggcaaac gcagcggcga cagcgcgacg atctcctgct ccgtcaacct cagtttgtcg
     3781 ttcagattgg tgtcgcggac cgggcctagc tcatagatgc ccgaaatcgc cagccccgcc
     3841 ttcaccagtc tatgtccgag gcacatcgct gtcagatgtc caccggccga ccagccggac
     3901 agcaccaccg gcccgttgag gccatgtgcc ggaccatgcg ccgccagcca gtccaacgcc
     3961 gcgttgatct ctgcgacgat ctcggtcagc gaggcgttcg gcgccagcgt gtagcccggc
     4021 aacgccgccg accaaccgcg cgcatacgcc ccggagatca ggttggcgaa ctgatccttg
     4081 ctgtttcgct gccagtagcc gccgtggatg aaaacgatgc acggcgcgtc ggggtcacgc
     4141 tgcgggaaca ggtcccacgt gttgcgctcc atcttgccgt agcgcaggtc gaggtgcagg
     4201 cagttggtgg tgcggaacac cgaggacgcc acttcgcgcg ccacattgag caccttgctg
     4261 tccgccaccg ccgccaggtt gtcataggcc gcgtcccgtt cggcctggct cagcagaccc
     4321 cacttcgcca gcacgctcat gcggttgctt tctggtacca gtccattatc tgccggcctt
     4381 gccagaacaa tgcgccgggc cggctggcaa tgtgcgccaa gacacgctcg aaccagccga
     4441 tccggtgcgg cacgccgctg atatacggat gtaccgcgag gcccatgacc ttggccccct
     4501 gctcgccagc ttcctcgtgc aaccgctcga actgcgcgat cgtgcggtcg aacaactcct
     4561 ccgctcggtg gtgctggatc atcatcatcg ggatgtcgtt gagttccacg ctgtagggca
     4621 tggtcagcat cggtccatgg gctgtgcgga tcgtggttgg caggtcgtcc accacccagt
     4681 cggcgcagta cttgatgccg gcttcggcga gcaggtccgg cgtgtccaaa tcttccgtca
     4741 agccgggagc gagccagccg acgggatcgt gacccccggc ctcccgcagc gcggcgatgg
     4801 cgtcgcggat catggcgcgc tggtccgcca caagatgggt ggccatttgg tggtggccgt
     4861 gcaccatgaa ctcccatccc gcatcgcggc aggcttttgt cacttgcgga taggccgagg
     4921 gaaccatgcc gttgatcgcc agggtgggcg cgatcccgtg cacgtccagg gcggctttca
     4981 accgccagaa accgacgcgc atgccgtatt cgtgccacgc ccagttgggc agatcgggga
     5041 tcacggcggc gccctgcggt gcgctcagca cctgtcgggg catggcgcgg tcgatgtccc
     5101 agcgctcgat gttcaccacc aggaagacgg cgacgcgctt gccgtctgga aggatcaggc
     5161 ggggacggtc gatagcggcc tggtagtcca ggcgctggtg gggtttctgc gggtctgtca
     5221 tgatcggatg atgaccgggg gaagccgttc gttcaagcag gtgatgccgg cttgcgcggc
     5281 g
//
LOCUS       DASWQN010000367         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1486507, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000367 DASWQN010000000
VERSION     DASWQN010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486507"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="VGC82_06040"
     CDS             <1..1428
                     /locus_tag="VGC82_06040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0422868.1"
                     /translation="DWVPYRRLGVTLRGRPFVFIIRIFEQARSGITMSPPVDPVPSDP
                     AIPETTRVVVIGGGIIGVCTAFFLARKGVPVVLCEKGEIAGEQSSRNWGWCRKMGRDP
                     RELPLAIEALRLWPEMNGLVGAETGFRRSGIVYLCRTQQELAKREAWLEQVGKPMQLD
                     SRLLTRDQASRVLPGLAGHWVAALFTPSDGRAEPAHAAPAIALAARRLGATILTRCAV
                     RGIETRGGSVAAVVTEHGRIACDQVVLAGGVWSQLFCRPLNLRLPQLKVLSSVMRTEK
                     LSGGPEASASGFGFGLRKRLDGGYTVASWSGNIADIVPDSFRFFGDFFPAMRLRGSDV
                     KLRFSSQFFHELAQPRRWALDRPSPFESERVLDPPAHPAILRQARVHVTEAFPVFHDM
                     RVAESWGGMIDVTPDGIPVISAVDALPGFFIATGFTGHGFGIAPGAGRLMAELVMGET
                     PVVDPLPFRYSRFSDGTRPKPSPLA"
     gene            1440..>2064
                     /locus_tag="VGC82_06045"
     CDS             1440..>2064
                     /locus_tag="VGC82_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY0422869.1"
                     /translation="MVNSFPTMRLRRLRSNQVMRDLLQEHTVSVNDLIYPIFVEEDLD
                     DFAPIESMPGVSRIPERKLGVAIKEIAAAGIKAVMTFGVSHHKDDIGSDAWNRDGLLA
                     RMIKRAKDAAPELIVISDTCFCEYTSHGHCGVIHGNDVHNDHTIENLGKQSVVAAEAG
                     ADMIAPSSMMDGQVMAIRTALDGNGFGNIPIMAYSSNFASAFYGQFRV"
BASE COUNT          350 a          656 c          677 g          381 t
ORIGIN      
        1 gactgggtac cttatcgccg actgggcgta acgctccgag gcagaccgtt cgtcttcatc
       61 atcagaattt tcgaacaagc ccgctcggga atcaccatgt ccccgcctgt tgatcccgtc
      121 ccgagcgatc ctgccattcc cgagacgact cgcgttgtgg tcatcggcgg cggcatcatt
      181 ggcgtctgca cggcgttctt tttggcccgc aagggcgtcc cggtcgtact gtgcgaaaag
      241 ggcgagatcg ccggggaaca gtccagccgc aactggggct ggtgccgcaa gatggggcgc
      301 gacccgcgcg agttgccgct ggcgatcgaa gcactgcggc tctggccaga gatgaatgga
      361 ctggtcggcg ccgaaaccgg cttccgccgc agcgggatcg tctatctgtg ccgcacccag
      421 caggaactcg cgaagcggga ggcatggctg gaacaggtcg gtaagccgat gcaactcgac
      481 agccgactgc tcacgcgcga ccaagccagc cgcgtgcttc ccggccttgc aggtcactgg
      541 gtagccgcgc ttttcacgcc cagcgatggc cgggccgaac ccgcccatgc ggcacccgcc
      601 atcgcattgg ccgcgaggcg tttgggtgcc acgatcctga cccgatgcgc agtgcgcggc
      661 atcgagaccc ggggcggaag cgtcgccgcg gtcgtgaccg aacatggccg gatcgcctgc
      721 gaccaggtcg tgctggccgg cggcgtctgg tcgcagctgt tctgtcgccc gctgaatttg
      781 aggttgccac agttgaaggt gttgtcgtcg gtcatgcgca ccgaaaagct gtccggcggt
      841 cccgaagcat cggccagcgg gtttggcttc ggcctgcgca agcgcctgga cggcggctac
      901 accgtcgcaa gctggagcgg caacattgcc gacatcgtgc ccgactcgtt ccgcttcttc
      961 ggtgatttct tcccagcgat gcggctgcgg ggcagcgacg tgaagctgcg cttcagcagc
     1021 cagtttttcc atgagcttgc ccagccgcgg cgctgggcgc tggaccgtcc gtcgccgttc
     1081 gagtccgaac gggtgctcga cccgcctgcg catccggcaa ttctgcggca ggcacgcgtc
     1141 catgtgacgg aagcgttccc agtcttccat gacatgcggg tcgcagaaag ctggggcggc
     1201 atgatcgacg tcaccccgga cggcatcccg gtgatctccg ccgtcgatgc gctgcccggg
     1261 tttttcatcg ccaccggctt tacaggccac ggcttcggca ttgcgcccgg cgccgggcgc
     1321 ctgatggcgg aactggtgat gggagaaacg ccggtggtcg atcccttacc tttccgctat
     1381 agccggttct cggatggaac tcggccgaaa ccttcgccgc ttgcttaggg ataactcgga
     1441 tggtcaacag cttccccacg atgcgactgc gccgcctgcg ctcgaaccag gtgatgcgcg
     1501 atctgctgca ggaacacacc gtctcggtga atgacctgat ctacccgatc ttcgtggaag
     1561 aggatctcga cgacttcgcg ccgatcgagt cgatgccggg cgtttcgcgc atcccggagc
     1621 ggaagctggg cgtcgcgatc aaggagatcg ccgcggccgg gatcaaggcg gtgatgacat
     1681 tcggcgtcag ccaccacaag gacgacatcg gcagcgacgc ctggaatcgg gatggactgc
     1741 tggctcggat gatcaagcgg gcgaaggatg cggcgccgga gctgatagtg atttccgaca
     1801 cctgcttttg cgaatacacc tcgcacggcc attgcggcgt gatccacggc aacgacgtgc
     1861 acaacgacca cacgatcgag aacctgggca agcagtctgt ggtggccgcg gaggccggtg
     1921 ccgacatgat cgcgccgtcc tcgatgatgg acggccaagt catggcgatc cgcaccgcgc
     1981 tggacggcaa tggcttcggc aacattccga tcatggctta ctcgtcgaat ttcgcgtctg
     2041 cgttctacgg gcagttccgg gtgg
//
LOCUS       DASWQN010000368         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_35846, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000368 DASWQN010000000
VERSION     DASWQN010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35846"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..874
                     /locus_tag="VGC82_06050"
     CDS             221..874
                     /locus_tag="VGC82_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEY0422870.1"
                     /translation="MHPPNPAVLFRDQRFVVLDKPAGLAVHAGPSGGPSVEDWFGALS
                     RRKDGPWLAHRLDADTAGCLVIALRRAALRSAQALFASGEVRKTYWAVVQGDVHGDAG
                     TVSAPLRRITSSEGWRMIVDERSGQKAVTDWQVCGRANGLTWLEMRPRTGRTHQIRVH
                     CTILGTPILGDERYGKAGGGLHLLARAIHIPLDPPVDALAQPPSHMRAALRLCGFRE"
     gene            complement(1109..>2083)
                     /locus_tag="VGC82_06055"
     CDS             complement(1109..>2083)
                     /locus_tag="VGC82_06055"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422871.1"
                     /translation="FVLGYDVAARLCGAFTPWPLAHQNGQACLLAAVAATARLRGLDA
                     AGISRTMRIAATLVLTPSYTNVVAGATALNVAGGMSGFAAALAPELALAGFEAQADAI
                     EESLGNLVGAGFKPEGLLDALGSTWHITQNYFRLYACCNPIHPALDALAEALAELRPS
                     AAEIDRIDIATYRFAAVMRNPDPPNYFASKYSLPHAAAAMIATGGAGHRALNDAALAD
                     PVIAALRHRVHITEEPAMTAMVPRLRPARVSVTLTDGRQATRTVASHRGDFNNPFAEA
                     EIRSKFRGLAGEVLTTDGVAAVESSVDGCEGWGNVGELAALMRRHGRA"
BASE COUNT          368 a          678 c          681 g          356 t
ORIGIN      
        1 gcctctcccc agccgtgcag tccgctttcg gtttcgacgc ggcagaacaa caggttcttt
       61 gcggtgcccg ggtcgaacag gaatgtttca atacgtgcga ttttcatgga ggtgctccct
      121 gcgggcactg tacgccgacc ccggggagca cggcaaacgg gcgctgccga accaagtggc
      181 ggggtagcgg ggcgaaccgg atattcgcta caccgcatcg atgcatccgc cgaacccggc
      241 cgtcctgttt cgggatcaac gcttcgtcgt gctggacaag ccggccggtc tggcggtgca
      301 tgccggtccg tcgggcggcc cgagcgtaga agactggttc ggcgcgctat cgcggcgaaa
      361 ggatgggcca tggctggcac accggctgga tgccgataca gccggttgtc tggtgattgc
      421 gctgcggcgg gcggcgctgc ggtcggccca ggcgctgttc gcatccggtg aggtgcgcaa
      481 gacctactgg gctgttgttc agggtgacgt tcacggtgat gcgggcaccg tctcggcgcc
      541 gctacggcgg atcaccagtt cggagggatg gcggatgatc gtggacgaac gatccggcca
      601 aaaggcggtg acggactggc aggtatgcgg gcgggcgaac gggctgacct ggctggaaat
      661 gcggccgagg acagggcgca cgcaccagat ccgcgtccat tgcacaatcc tcgggacgcc
      721 gatcctgggg gacgaacgat acggcaaggc cggcggcggg ctgcacctac tggcacgggc
      781 aatccacatt ccgctcgatc ccccggtcga tgccctcgcg caaccgccct cgcatatgcg
      841 tgccgccctg cgactttgcg ggtttcgtga gtagggtcag ggcaggctca ggattatcat
      901 ccccgactcg accgattgca ctgcgccggg acgatttgtg ttactggcaa ccgtgatctc
      961 ttgtggtcgc agagcaagag gtgggtggcc gtgccaagca tggccacgac aatattatca
     1021 acgacaccat tgtcaattca ggatgaatgc gagccagtcg gccgcctgac ttccattcgc
     1081 catggccgag cgcggccata tatggcgcct acgcccgtcc gtgacgccgc atcagcgctg
     1141 ccagctcgcc gacgttgccc cagccctcgc agccgtcgac cgaggattcc accgcggcga
     1201 caccatccgt cgtcagcact tcaccggcca agccccggaa ttttgaccga atttccgcct
     1261 cggcgaatgg gttgttgaaa tcgcctctat ggctcgcgac ggtccgcgtc gcctgccgcc
     1321 catccgtcag cgtgacgctc acccgagcag gccgtaggcg gggcaccatg gcggtcatcg
     1381 ccggctcctc ggtaatgtgc acacgatgac gcagcgccgc gatgaccgga tccgccaaag
     1441 cagcatcgtt caatgcacgg tgcccagcgc cacccgtcgc gatcatcgcg gcggcggcat
     1501 gcggcagcga atacttcgag gcgaagtaat tcggcggatc cgggttgcgc atcaccgcag
     1561 caaagcgata cgtcgcgata tcgatgcgat cgatttccgc tgccgagggc cgcagctccg
     1621 ccagtgcttc ggccagcgca tccagtgccg ggtgaatcgg attgcagcac gcgtacagcc
     1681 ggaaatagtt ctgcgtgatg tgccaggtcg agccgagagc atcgagcaat ccctctggct
     1741 tgaagccggc tccgaccagg ttgccgaggg attcctcgat cgcatccgcc tgcgcctcga
     1801 atcccgccag ggcaagttcc ggggccagcg cggcggcaaa tccgctcatg ccccccgcca
     1861 cgttcaatgc ggtcgcaccc gccacgacgt ttgtatagct gggcgtcagg accaaggtcg
     1921 ccgcgatgcg catcgtccgg ctgatgccgg cggcatccag gccacgcagc ctggccgtcg
     1981 ccgcgaccgc tgccaacagg caggcctggc cgttctgatg tgcgagaggc cagggcgtga
     2041 acgcgccgca aagcctggcg gcaacatcat aacccagcac gaa
//
LOCUS       DASWQN010000369         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_35889, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000369 DASWQN010000000
VERSION     DASWQN010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35889"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /gene="kdpB"
                     /locus_tag="VGC82_06060"
     CDS             complement(<1..1266)
                     /gene="kdpB"
                     /locus_tag="VGC82_06060"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEY0422872.1"
                     /translation="MEARKRDQKSNLLDPAILLPALGQAVRKLDPRLMIRSPVMFVVE
                     VAATLTTAIFVRDLIVGGAHLGFTFQIILWLWITVLFAKFAEAVAEGRGKAQAASLRR
                     ARTDTMANRVTAAGTETVAAPELRQGDIVIVAAGELIPSDGEVIEGIASIDESAITGE
                     SAPVIRESGGDRSAVTGGTRVLSDWIKVRITAAQGSTFLDRMIALVEGAERQKTPNEI
                     ALNILLAGLTIIFVFAVATIPSFATYAGGSVSVVILVALFIALIPTTIGALLSAIGIA
                     GMDRLVRFNVLAMSGRAVEAAGDVDTLLLDKTGTVTLGNRQATEFIPLTGVSARELAD
                     AAQLSSLPDETPEGRSIVVLAKEKYDIRERDMAPMGARFVPFTAQTRLSGIDFDRSSI
                     RKGAVDAVLEWVRTPTGSSHGGIATVVRPD"
     gene            complement(1333..>2078)
                     /locus_tag="VGC82_06065"
     CDS             complement(1333..>2078)
                     /locus_tag="VGC82_06065"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY0422873.1"
                     /translation="GNMEGKEVRFGIANSALFATITTDASCGAVTSMHDSLTPLGGMV
                     PMINIMLGEIIFGGVGSGLYGMLLFVIVAMFVAGLMVGRTPEYLGKKLQAKEVKMAML
                     AILILPLSILGFTALAVVIQPGLSGLANAGPHGFSEALYAYVSATGNNGSAFAGLSAN
                     TPFWNSTLGIAMFIGRFLMIVPMLAIAGSLAAKKIVPVSAGTFPTDGGLFVGLVVGAI
                     LVTGGLTFLPAIALGPVVEHLSMLSGTAF"
BASE COUNT          403 a          685 c          648 g          342 t
ORIGIN      
        1 atcgggtcgg accacggtgg cgatgccgcc gtggctgctg ccggtcggtg tccggaccca
       61 ttcgagcacc gcatccaccg cgcccttgcg gatggacgaa cgatcgaagt cgatgcccga
      121 caaccgggtc tgcgcggtga acgggacgaa acgcgcgccc atcggcgcca tatcgcgctc
      181 gcgaatgtcg tatttttctt tcgccagcac gacgatcgag cggccctcgg gcgtttcgtc
      241 cggcagcgac gacaactgtg ccgcatcggc caattcccga gcgctgacac ccgtaagcgg
      301 aatgaattcg gtcgcctggc gatttcccaa ggtgaccgtt ccggtcttgt ccagcagcaa
      361 cgtatccacg tccccagccg cctccacggc tcggccggac atcgccagga cgttgaaccg
      421 caccaggcga tccatgccgg cgatgccgat cgccgacagc agcgcgccga tcgtcgtcgg
      481 gatcagggcg atgaacaggg ccaccagtat caccacggac accgatccgc cggcataggt
      541 ggcgaaactc gggatcgtgg cgaccgcgaa gacgaagatg atcgtcaggc cggccagcag
      601 gatgttcaac gcgatctcgt tcggcgtctt ctgccgttcg gcgccctcga ccaaggcgat
      661 catgcggtcc aggaaggtcg agccctgggc cgcggtgatg cgcaccttga tccagtccga
      721 tagcacccgc gtgccgccgg tcaccgccga ccggtcgccg ccgctctcgc ggatcaccgg
      781 tgcggactct ccggtgatgg cggactcgtc gatcgaggcg atgccttcga tcacctcgcc
      841 atcgctgggg ataagttcgc cggctgccac tatgacgatg tcgccttgcc gcaattccgg
      901 agcagcgacg gtttcggtgc cggcggcggt cacgcggttg gccatggtat ccgttcgcgc
      961 acgccgcagc gatgcggcct gggctttgcc gcggccctcg gcgaccgcct cggcgaactt
     1021 ggcgaaaagg acggtgatcc acagccacag gatgatctgg aacgtaaagc ccaaatgggc
     1081 accgccaacg atcaaatcgc gcacgaagat cgccgtcgtc agggttgcgg cgacctccac
     1141 cacgaacatc actggactgc ggatcatcaa cctggggtcg agtttccgga ccgcctgacc
     1201 aagcgccggc agcaggatgg cggggtcgag cagattcgat ttctggtcgc gtttacgggc
     1261 ttccatcaca ggataccttg tgtttaatcg cacgattcac gcgcttcggc gttccgccga
     1321 gcgcgatcgt gctcagaaag ctgtgccgga gagcatcgac agatgttcga ccaccggccc
     1381 aagggcgatg gccggaagaa acgtcaagcc gccggtaacc aggatcgcgc cgaccaccag
     1441 gccgacgaac agaccgccat ctgtgggaaa cgtccccgcc gaaaccggga cgatcttctt
     1501 cgccgccagc gatccggcga tggcgagcat cggcacgatc atcaggaagc ggccgatgaa
     1561 catcgcaatg cccaacgtcg agttccagaa cggcgtgttc gcggacagtc cggcgaaggc
     1621 gctgccgttg ttgccggtgg ccgaaacata agcataaagc gcctcggaaa atccatgcgg
     1681 cccggcattg gcgagcccgc tcagtcccgg ctggatcacc accgcgagcg ccgtgaagcc
     1741 aaggatggac aacggaagta tcaggatcgc cagcatggcc atcttgacct ccttcgcctg
     1801 gagcttcttg ccgaggtatt caggggtgcg tccgaccatc aggccagcca cgaacatcgc
     1861 cacgatgacg aacagcagca tgccgtacag gcccgacccg acgccgccga agatgatttc
     1921 gcccagcatg atgttgatca tcggaaccat gccgcccaac ggcgtcaggc tgtcatgcat
     1981 cgaggtaacg gcgccgcagg acgcgtcggt ggtgatcgtc gcgaacagcg ccgagttggc
     2041 gatgccgaag cgaacctcct tcccttccat gttgccgg
//
LOCUS       DASWQN010000370         2584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1486755, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000370 DASWQN010000000
VERSION     DASWQN010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486755"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VGC82_06070"
     CDS             <1..585
                     /locus_tag="VGC82_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0422874.1"
                     /translation="EVVQGINAFALGMQSVNPKARLKFILINSWYDPPKEGDAAKALI
                     DQGCDIITQHTDSPAPLQAASARGIKAFGQSTDMAKFAPGTHLTASTDNWGPYYIKRI
                     QDVLSGTWKSTDTWGGFDSGMLEMAPFANMPPDVAALATATVADITAGKTTVFTGPIM
                     DQSGAVKVAAGTTMDDGALNAMQWLVQGVDGKLS"
     gene            652..2205
                     /locus_tag="VGC82_06075"
     CDS             652..2205
                     /locus_tag="VGC82_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0422875.1"
                     /translation="MIERPPPSPLLTMTGMTKQYPGCLANDNVALTVERNQIHALLGE
                     NGAGKSTLVKMIYGVVKPDAGEVVWQGQKVVIQSPGHAQRLGIGMVFQHFSLFEALTV
                     TENIALGLATAKERAGLRERIVDVSNEYGLPLDPDRVVHDLSVGERQRIEIVRCLLQN
                     PKLLIMDEPTSVLTPAEVRKLFVTLRRLAAEGCSILYISHKLEEVRALCSRATIMRLG
                     RNVAECAPAEKTVKELAEIMINMALLSPTPRPLAANAAPPRLVLDRLTVRSPHHFGTD
                     LKEVSLSLAGGEILGIAGIAGNGQSELMDALSGEILAGRPESVMIDGVAAGHMGPHER
                     RRIRGCFVPEERSGHGAVTTMTLAENAFLSGFVRQALVRFGVIDTKKTAAFATHIITH
                     FDVRTTGPKAEARSLSGGNLQKFLVGREVLQEPSVLVINQPTWGVDAGAALAIHEAIM
                     ALAQAGAAVMIISQDLDEIFVLADRIAVIAGGHLSAPVPTRQATVESIGRLMGGDAVA
                     PAPGEMEVV"
     gene            2268..>2584
                     /locus_tag="VGC82_06080"
     CDS             2268..>2584
                     /locus_tag="VGC82_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0422876.1"
                     /translation="MLKIEARGYQSKFWRYASPVLALLLTGATSGLIFAAMGRPPGLT
                     VYTFLIAPLLQQDGFEALVVKAAPLIMMGVGLSLAYRANVWNIGTDGQFTLGAICGGG
                     VALA"
BASE COUNT          502 a          793 c          845 g          444 t
ORIGIN      
        1 gaagtcgtcc aaggcatcaa tgctttcgca ctcggtatgc agtcagtcaa cccaaaggct
       61 cgtttgaagt ttatcttgat taattcctgg tacgacccgc cgaaggaagg cgacgcggcg
      121 aaggcgctga tcgaccaggg ctgcgacatc atcactcagc acaccgactc gccggcaccc
      181 ctgcaggccg cttcggcccg cggcatcaaa gcattcgggc agtccaccga catggcgaaa
      241 ttcgcccccg gcacccactt gaccgcaagc accgacaact ggggaccata ttacatcaag
      301 cgcatacagg acgtgctgtc aggtacctgg aaaagcaccg atacatgggg tggcttcgac
      361 agcggtatgc tggagatggc cccgttcgcc aacatgccgc cggatgtcgc ggcgctggca
      421 acggcgaccg tggcggatat cacagccggt aaaacgacgg tcttcaccgg cccgatcatg
      481 gaccaaagcg gtgcggtcaa ggtcgcggcc ggaacgacga tggacgacgg cgcactgaac
      541 gcgatgcagt ggctggtcca gggcgttgac ggcaaactaa gttgaatttc gttccgggtc
      601 cgggtagatc gtcacgtcac aaacggttcg gatccacgca atgagcttcg catgatcgag
      661 cgaccaccgc ccagcccgct gctgacgatg accggtatga ccaagcaata cccgggctgc
      721 ctcgcgaacg acaacgttgc actcacggtg gaacgcaacc agatccacgc gctgctcggc
      781 gagaacgggg cgggcaaaag cacgctggtg aaaatgatct acggcgtggt caagcccgac
      841 gccggcgagg tcgtctggca aggccagaag gtcgtcatcc aaagtcccgg tcatgcgcaa
      901 cgcctcggca tcggcatggt gttccagcat ttctcgctgt tcgaagcgct gaccgtcacc
      961 gaaaacatcg cgctcggtct tgccaccgcc aaggagcgtg ccggcttgcg cgaacggatc
     1021 gtagatgtct cgaacgaata tggcctgccg ctcgatccgg atcgggtggt gcacgatctt
     1081 tccgtcggcg aacgccagcg gatcgagatc gtccgctgcc tgctgcaaaa tcccaaactg
     1141 ctgatcatgg atgaaccgac ctcggtgctg acgccggcgg aagtgcggaa gctgttcgtc
     1201 acactgcgcc gactggccgc cgaggggtgc tcgatccttt atatcagcca caagctcgaa
     1261 gaagtccgcg cgctgtgttc cagggcgacg atcatgcgac tggggcgcaa cgtcgccgag
     1321 tgcgcgccgg ccgagaaaac ggtcaaggaa ctggccgaga tcatgatcaa catggcgctg
     1381 ctgtcgccga cgccacggcc gcttgcggca aatgcggcac cgccgcggct tgtgctggat
     1441 cggctgaccg tccgctcgcc gcaccacttc ggcaccgatc tgaaggaggt ttcgctgtcg
     1501 ctggcaggtg gggaaatcct tgggatcgca ggcatcgcgg gcaacggaca gagcgaactg
     1561 atggatgcgc tgtcaggaga aatcctggcc ggccggcccg aaagcgtcat gatcgacggc
     1621 gtggccgccg ggcacatggg tccgcatgag cggcggcgca tccgcggctg cttcgtgccg
     1681 gaggaacgca gcggccatgg cgccgtgaca accatgacgc tggcggaaaa tgccttcctg
     1741 tcggggttcg tgcgccaggc tctggtacga ttcggggtga tcgatacgaa aaagaccgca
     1801 gccttcgcga cccacatcat cacgcatttc gatgtccgca ccaccggccc gaaggcggag
     1861 gcccggtcgc tgtcgggcgg gaatttgcaa aagttcctgg tgggacgaga ggttttgcag
     1921 gaacccagcg tgctggtgat caatcagccg acctggggcg tggacgcagg ggcggcgttg
     1981 gccatccacg aggccatcat ggcgctggcc caggccgggg cggctgtgat gatcatctcc
     2041 caggacctgg acgagatttt cgtgctggcc gaccggatcg cggtgatcgc cggaggccac
     2101 ctatccgcgc cggtgccgac ccgccaggcg acggtcgaat cgatcggtcg gctgatgggg
     2161 ggcgacgcgg tggcgccggc cccgggggag atggaggttg tttagccgga ccagcgttcg
     2221 aaccggccag gcgtgccaac atcccgccgt cgccggggga cgaactcatg cttaaaatcg
     2281 aggcccgcgg ctatcaatcg aagttctggc gctatgcctc gccggtcttg gcactgttgc
     2341 tgaccggagc gacgtccggg ttgatcttcg ccgccatggg ccgcccaccc ggcctgaccg
     2401 tctatacatt cctgatcgcg ccattgctgc agcaggacgg cttcgaggct ctggtggtca
     2461 aggcggcacc cctgatcatg atgggtgtcg gactgtcgct agcttacaga gccaatgtgt
     2521 ggaacatcgg caccgatggg cagttcacgc tgggtgccat ctgcggcggc ggcgtggcac
     2581 tggc
//
LOCUS       DASWQN010000371         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1090892, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000371 DASWQN010000000
VERSION     DASWQN010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090892"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VGC82_06085"
     CDS             <1..431
                     /locus_tag="VGC82_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0422877.1"
                     /translation="PRYQSLQPHYSLVERAEFEGPLENLCLAEKIGVIGYYSLASGFL
                     TGKYRSQADTQGQARGSRVQKYLDDYGFSVLAALDEVAARYEAKPGQIALAWLIARPS
                     VTAPIASATNLQQLAELVEAAEIELDADSIQKIDTASQPR"
     gene            544..783
                     /locus_tag="VGC82_06090"
     CDS             544..783
                     /locus_tag="VGC82_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY0422878.1"
                     /translation="MDHAVSAADANRGFSHILREVRDGASYVVTAHGKPVARIVPCEL
                     ADAARAAARSALLERLRDQPVIDIGPWRRDELYER"
     gene            787..1197
                     /locus_tag="VGC82_06095"
     CDS             787..1197
                     /locus_tag="VGC82_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0422879.1"
                     /translation="MRVALDTNLLVYAEGVNGDDRKLRAHDALTRFASDDIVVPAQAL
                     AELFTVLTRKARWPAAQAQAAVLTWYDACQVADTTSSVLLEAMNLAAMHQYSLWDSVM
                     LASAAQAGCRLLLSEDMQDGFIWRGVEIRNPFRI"
     gene            complement(1201..2046)
                     /gene="nadC"
                     /locus_tag="VGC82_06100"
     CDS             complement(1201..2046)
                     /gene="nadC"
                     /locus_tag="VGC82_06100"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY0422880.1"
                     /translation="MPSLPMVMIEPAVRAALLEDLGRAGDLTTDAIVPADALTRTALV
                     ARQPGVVAGLDFAAAAFRLIDPAIAVDVALPDGSRLRQGDLIATISGPARGILTAERT
                     ALNFLCHLSGVASATRGIADAIEGTRAQVCCTRKTMPGLRFAQKYAVRVGGGSNHRFG
                     LDDAVLIKDNHVAIAGGVRPAVERARAGVGHLVKIELEVDTLAQLEEALTLGVDAVLL
                     DNMGPDMLRQAVAMVDGRAITEASGRIRPDTAPAIAASGVDLLSIGWLTHSATVLDIG
                     LDWLE"
     gene            complement(2046..>2443)
                     /gene="nadA"
                     /locus_tag="VGC82_06105"
     CDS             complement(2046..>2443)
                     /gene="nadA"
                     /locus_tag="VGC82_06105"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY0422881.1"
                     /translation="HCEVHERFTAAEVRALRTDYPGVVILAHPECPPEVVAEADYTGS
                     TADMSGYVGRERPARVVLLTECSMADNVSVGYPDIEFVRPCNLCPHMKRITLPKIRRA
                     LETMQEEVTIDPALINRARRSVERMLEVR"
BASE COUNT          439 a          806 c          761 g          437 t
ORIGIN      
        1 ttccgcgcta ccaaagcctg cagccgcact acagtctggt agagcgcgcg gagtttgaag
       61 gtccattgga gaatctctgc ctggctgaga agatcggagt gatcgggtac tactccttgg
      121 ccagcgggtt tttgaccgga aagtaccgat cgcaggccga cacccagggc caggcccgtg
      181 gatcacgcgt gcaaaaatac ctggacgact atgggttcag cgttctggcg gcgctggatg
      241 aggtggcggc tcgttacgag gctaaacccg gccagatcgc cctggcgtgg ctgatcgcgc
      301 ggccgagcgt tacggcacca atcgcgagcg ccacaaatct gcagcagctt gccgaactgg
      361 tcgaagcggc tgaaatcgag ttggatgccg actcgatcca gaagatcgat acggccagtc
      421 aaccgagata aactgactcc cggtacgggc gccggcatgt ctcgaggtcg gcaggatcaa
      481 aagcggcttg cggtctgagg gtaaatagat taaatagtga aaatcactac caggagcgtt
      541 gttatggatc acgctgtctc cgctgccgat gccaatcgcg gtttctcgca tattttgcgg
      601 gaggttcggg atggcgcgtc ctacgtcgtg acggctcacg gcaaaccggt tgctcgcatt
      661 gtcccctgcg aactggcgga tgcagcacgg gccgccgcac gttcggcttt gctggagcgt
      721 ttgcgggacc agccggttat cgatatcggg ccctggcgcc gtgacgagct gtatgagcgt
      781 tgacgcgtgc gcgtcgcgct cgacaccaat ctgcttgtgt atgccgaagg ggtaaacggg
      841 gatgatcgca agctaagggc acatgacgct ctgacccgtt ttgcttccga cgatatcgtg
      901 gtgccggctc aggcgctcgc cgaactgttc actgtgctga cacgcaaggc tcgatggccg
      961 gcggcgcagg cacaagccgc ggtgctgacg tggtacgacg cgtgccaggt ggccgacacc
     1021 acgtcctcgg tcctgctgga ggcgatgaac ctggcggcaa tgcaccagta ctcgctgtgg
     1081 gactcggtca tgctcgccag cgcggcccaa gccggctgcc gcttgctgct gtccgaggac
     1141 atgcaggacg ggtttatctg gcgaggcgtg gagatccgga acccgtttcg gatataaccc
     1201 tcactccagc caatccagcc cgatgtccag cactgtcgcg ctgtgcgtca gccagcctat
     1261 ggacagcaga tccaccccgc tggctgctat cgccggcgcg gtatccggcc gaatccgtcc
     1321 ggacgcctcg gtgatcgccc ggccatcgac catcgcgacc gcctggcgca acatgtctgg
     1381 acccatgttg tccagcagca cggcatccac ccccagcgtc aacgcttcct ccaactgggc
     1441 cagcgtatcg acctccagtt cgattttcac cagatgtccg acgccggcgc gggcgcgctc
     1501 caccgccggc cgcacgccgc cggcgatggc gacgtggttg tccttgatca gtaccgcgtc
     1561 atccagcccg aaccggtggt tcgacccgcc gccaaccctt actgcgtatt tctgcgcgaa
     1621 ccgcagccca ggcatggtct tgcgcgtgca gcagacctgc gcccgtgtcc cctcgatcgc
     1681 atcggcgatg ccgcgggttg ccgatgcgac gccgctgagg tggcacagga aattcagcgc
     1741 ggtgcgttca gccgtcagga tgccgcgggc ggggccgctg atcgtcgcaa tcaggtcgcc
     1801 ctgacgcagg cggctgccat ccggcagggc gacatccacg gcaatcgccg gatcgatcag
     1861 ccggaatgcc gccgcagcga agtccagacc cgcgaccacc cctggctgcc gcgccaccag
     1921 ggccgtgcgg gtcaacgcat cggccggaac aatggcatcg gtcgtcagat cgcccgcccg
     1981 ccccagatcc tccagcagcg cggcccgcac cgctggctcg atcatcacca ttggcaggga
     2041 cggcatcagc gaacttccag catgcgttcg accgaccgtc tggcccggtt gatcaaagcc
     2101 ggatcgatcg tcacctcctc ctgcatcgtc tccagtgccc ggcggatttt cggcagcgtg
     2161 atgcgcttca tgtgcgggca cagattgcac ggccgcacaa actcgatatc cggatatccc
     2221 accgaaacat tgtccgccat tgagcattcc gtcagcagca cgacccgcgc cggcctctct
     2281 cgccccacat agccggacat gtcggcggtc gaaccggtgt agtcggcctc ggcgaccacc
     2341 tccggcgggc attccggatg cgccaggatc accacgccgg ggtagtcggt gcgcagggca
     2401 cgcacctcgg cggcggtaaa gcgttcatgc acctcgcaat gcc
//
LOCUS       DASWQN010000372         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_827850, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000372 DASWQN010000000
VERSION     DASWQN010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827850"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VGC82_06110"
     CDS             <1..442
                     /locus_tag="VGC82_06110"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0422882.1"
                     /translation="APVASAVPPATMQALRKKAHAKLAHGLGAAAARELLEETGLSLG
                     TPPRLDGLHYLARAVTPPGSPIRFNARFLVVEERHVSGSLGGDGELEALRFYGMTEAL
                     ALELALPTRRVLERLKTMLALPEAERFTQIQTPVLLRDRGWIME"
     gene            503..>1737
                     /locus_tag="VGC82_06115"
     CDS             503..>1737
                     /locus_tag="VGC82_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0422883.1"
                     /translation="MTDLHLLTIAQASDRIKARTLSPVELTEAFLARVKRFDNRLNSH
                     LLVLEEQALADARTAESEIVAGRWRGRLHGIPIGLKDIYNTAGIPTTGHSALFKDHVP
                     TEDAFTVSLLRKAGAVITSKLATWEFAIGGSSFDLPWPPARNPWDVSLDPSGSSSGSA
                     VAVAAGLCLGAMGSDTGGSIRGPAAWCGIAGHKPTYGLLSRRGILPLSFSLDHAGPMC
                     WTSEDCALMMQVLASHDPLDAGSAAVPAVDFTAGLGTGLSGLRVGVVRHFFEKDLLTD
                     PETIAVLEVSVLALRDMGATVGDVKLSPFGVYADCGSLISRSEAYAIHQHWLRTTPDL
                     YGSFGRHRLMAGAFILAADYINAQRERTRLVAELAETMRSVDVLIFPTARCPARPIGE
                     DSMASGFQPFFNRAFNVTG"
BASE COUNT          311 a          535 c          558 g          333 t
ORIGIN      
        1 cgcgcccgtc gcgagtgctg taccgcctgc gacgatgcag gcattgcgca agaaggcgca
       61 tgcgaagctg gcacatggcc tgggtgccgc ggccgcccgc gaactgctgg aggaaaccgg
      121 cctctcgctc ggcacacccc cccggctcga tgggctgcat tacctcgctc gggcagtcac
      181 tccaccgggt tcgccgatcc gcttcaatgc ccggtttctg gtggtggagg aacgccatgt
      241 cagcggcagc ctcggtggcg acggagagct ggaagcattg cgattctacg gcatgaccga
      301 ggcgctggca ctggaactcg cactgccgac gcgccgcgtg ctggagcggt tgaagacaat
      361 gctcgcattg ccggaggcag aacgattcac ccagatccag acaccggttt tgttgcgcga
      421 tcgcggctgg atcatggaat agcagccgct tggtatcggt cttgcttaac cttcagcgaa
      481 cgtccgcaag ctgaaggcat tcatgaccga tttgcacctt ctgaccattg cccaggcatc
      541 cgaccggatc aaggcccgca cgttatcgcc ggttgagctc accgaggcct tccttgctcg
      601 ggtcaagcgc ttcgacaaca ggttgaacag ccatttgctg gtgctggaag aacaggcact
      661 ggctgacgca agaacagcag aatccgagat cgtggccggc cgatggagag gaaggctgca
      721 cggcatcccg atcgggttga aggatatcta caacaccgcg ggcatcccca cgactggcca
      781 ctcggcgttg ttcaaggacc acgttccgac cgaggatgcc ttcaccgtaa gcctgttgcg
      841 caaagcaggt gccgtcataa cgagcaagct ggcgacatgg gaattcgcca ttggcggatc
      901 gtcgttcgat ctgccctggc cgccggcgcg caacccttgg gatgtctcgc tcgacccatc
      961 cggatcgtcg tcgggatcgg ctgtcgcggt tgccgccgga ttatgcctgg gggcgatggg
     1021 cagcgacacc ggcggttcga tccgcgggcc ggcggcctgg tgtggaatcg ccggccacaa
     1081 gccaacctat ggtctgctga gccgcagggg gatcctgccg ctttcgtttt cgctggatca
     1141 tgccggcccg atgtgctgga ccagcgaaga ttgcgcgctg atgatgcagg ttcttgccag
     1201 tcatgacccc ttggacgccg gtagcgccgc agttccagca gtcgatttca cggcaggatt
     1261 gggcaccggc ttgtcgggac ttcgggtcgg ggtcgtgcgg cattttttcg aaaaggatct
     1321 tctgactgac ccagaaacga tcgcggtgct ggaagtcagc gttctcgccc tgcgcgacat
     1381 gggagcgacc gtcggcgatg taaaattgtc cccgtttggt gtttacgccg actgcggcag
     1441 cctgatctcg cgcagcgaag cttatgcgat ccaccagcac tggctgcgca cgacgccgga
     1501 cctgtacggc tcgtttggcc gccaccgttt gatggcgggg gccttcatct tggccgccga
     1561 ctacatcaac gcccagcggg aacggacacg cctggtcgcg gaactggcgg aaaccatgag
     1621 gagtgtggac gtgctgatct tcccgacagc ccgctgcccg gcgcgcccga tcggcgagga
     1681 ctcgatggca tcgggattcc agccgttctt caaccgagcg ttcaatgtga ccggcag
//
LOCUS       DASWQN010000373         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_431745, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000373 DASWQN010000000
VERSION     DASWQN010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431745"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1338
                     /gene="dnaN"
                     /locus_tag="VGC82_06120"
     CDS             214..1338
                     /gene="dnaN"
                     /locus_tag="VGC82_06120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY0422884.1"
                     /translation="MKLKADRATLIKALAHVQSVVERRNTIPILANVMIAVREGKLTL
                     TATDMEIAIVEDVPASSSRNGACTAPAATLYEIVRKLPDGAEVELDHPGGDAQLALRA
                     GRYDTKLTVLPVEDFPSMTAGSLPHKFSLGAQTLRALIDRTKFAISTEETRYYLNGIY
                     LHHADSDGTPVLRAVATDGHRLARVEEPLPEGAASMPGVIIPRKTVNELRKLLDEVSG
                     NVEIALSDTRIQFRADQMLLTSKLIDGTFPEYERVIPRGNDKVLRVGKKDFADAVARV
                     AAISSERSRPVKLSLARDLLVLSASSPEQGTATEELDGDRVKYDAGPLEIGFQARYLN
                     DITDQIPEQVEFRFADGSAPTVVQDAGDASALYVLMPMRV"
     gene            complement(1669..>2094)
                     /locus_tag="VGC82_06125"
     CDS             complement(1669..>2094)
                     /locus_tag="VGC82_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY0422885.1"
                     /translation="LVSFLTDIIGVQDTGTAASGATVTYHDSCSGLRELGIKRQPRDL
                     LKAAGIAVTEMAEPEICCGFGGTFCVKYPDISVRMVSDKTKDIVATGAETVLAGDLGC
                     LLNIAGRLQREGHGVQARHVAEVLAGMTDDLPAIGQARR"
BASE COUNT          395 a          688 c          644 g          367 t
ORIGIN      
        1 gcagatcctg atcaccggtg tcgaggatgt gctggggccg cttttggcgg cacgatagcg
       61 gccgcacctg cctgagcgtc cgtccgcgtg gatggccgtg cgacggtcct tggcggatgt
      121 tgatatgaat tgctagactg gctcctacga gtcgcttgct tccccgctgt ccgcggccca
      181 gaagcgaggc tggccctatg taggaacctg acaatgaagc tgaaggccga ccgcgccacc
      241 ttgatcaagg cgctggccca cgtccaaagc gtggttgaac ggcgcaacac catcccgatc
      301 ctggcgaacg tcatgatcgc ggtgcgcgag ggcaagctta cgctgacggc caccgacatg
      361 gaaatcgcca tcgtggagga cgttccggcc tcgtcctccc gcaacggtgc ctgcaccgca
      421 ccagccgcga cgttgtatga gatcgtgcgc aaacttcccg atggcgcgga ggtcgagctc
      481 gaccatcccg gcggcgacgc ccagctcgcg ctgcgtgccg gacggtacga caccaagctg
      541 accgtgctgc cggtcgagga cttcccgtcc atgaccgcgg gcagcctacc gcacaaattc
      601 agcctgggcg cgcaaacgct gcgggcgctg atcgaccgca cgaagttcgc aatcagcacg
      661 gaggaaaccc gctactacct gaacggcatc tacctgcacc acgccgatag cgacggcaca
      721 ccggtgctgc gtgcggtcgc caccgacggt catcgcctcg cccgggtcga ggaaccactg
      781 cccgaaggtg cggcgtcgat gccgggcgtg atcattcccc gcaagacggt gaacgaactt
      841 cgaaaactgt tagatgaggt gagcgggaac gtggagatcg ccctgtccga cacccgtatc
      901 cagttccgcg ccgaccagat gctgctcaca agcaagctga tcgacggcac gttcccggaa
      961 tacgaacggg tgatcccacg cggcaacgac aaggtgctgc gtgtgggaaa gaaggatttc
     1021 gccgatgccg tggcgcgtgt ggccgcgatc tcctcggaac ggtcgcgtcc ggtgaagctg
     1081 tcactggcgc gcgatctcct ggtgctgtcc gcttcaagcc cggaacaggg taccgcgacc
     1141 gaggaactgg acggcgaccg cgtgaagtac gatgccggcc cgctggaaat cggctttcag
     1201 gcgcgctacc tgaacgacat caccgaccag atccccgagc aggtggaatt ccggttcgcc
     1261 gatgggtcgg cgccgacggt ggtgcaggat gcaggggatg ccagtgcctt gtatgtgctg
     1321 atgccgatgc gggtttagac ccgcgcccag tcaaggggca ttccccagga gaaaagatag
     1381 gctccggccc cgcagccatc tatcgctttt aagatgatga ctgctttcaa gaggataacc
     1441 gcttcaagcc gggtgatcct gctgggcgat atcgtggcgt gctttgcctc atgtgcgccg
     1501 agtccgctgt cgtcgcttgg ttgccgcttg cgtcttggcc ggccctccac aactttcccc
     1561 tgtcgaggca aattcgtgga tggccgccag gcacggccat aacaccacac ccgccacaac
     1621 agaaccggac agcgcggccg aggcaggtat ttcggcgccg acccggatct aacggcgcgc
     1681 ctgcccaatc gccggcaagt cgtccgtcat tcccgccagg acttcggcga cgtggcgagc
     1741 ctgaacgccg tgcccctcac gctgaagccg cccggctata ttcagcaggc atcccaagtc
     1801 gccggccagg accgtttcgg cgccggtggc gacgatgtcc ttggttttat ccgaaaccat
     1861 tcgcaccgag atatcgggat acttgacgca aaacgtcccc ccgaacccgc aacagatttc
     1921 cggttcggcc atctcggtca cggcgatccc agcagccttc agcaagtcgc gcggctgccg
     1981 cttgatcccc agctcccgca gtcccgaaca actgtcgtga tacgttacgg tcgcaccgga
     2041 tgctgccgtc ccggtgtcct ggacgccgat gatatccgtc aggaaactca ccag
//
LOCUS       DASWQN010000374         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2543295, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000374 DASWQN010000000
VERSION     DASWQN010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543295"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(458..>1534)
                     /locus_tag="VGC82_06130"
     CDS             complement(458..>1534)
                     /locus_tag="VGC82_06130"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0422886.1"
                     /translation="QEEELRGIALGMKDAGSGMLEMVSEWAPDHNEEFAMLRRVIEAC
                     GRPAVFTLTQRHSRPEVWRDLIKHADKAARDGLPIRPVVAPRAIGVLLGLAGSQNPFS
                     GTPTYKTIAHLPVAERARTMADPEVRAKILSEDPKEGNTFPLFHRISFDFMFRFGNPP
                     NYQPKMEDSISAIAARGNRTPQEVAYDMLIEDDGANFIYHPLGNYAYGNLSMPETLLD
                     NRNCIMGLGDGGAHVAFILDAGYQTWMLSHWGRDQKRWDVAEMIRRVTSDTAGAAGLH
                     DRGVLAIGKKADVNVIDWGKLGSGAPYVVNDLPAGGKRLMQKVSGYDATIVSGRVTFR
                     EGQATGTLPGKLVRGPQTELAPGV"
BASE COUNT          263 a          513 c          470 g          288 t
ORIGIN      
        1 gtggtcgggc tgttctatgt gcggttgagc tgggaggttc gtgtggaggc tggccgctgc
       61 gcttcaacac ggatggcacg gaagtcgaca cggaggctcg gagaaggcga tccagcctcg
      121 ttgttgatga tcggcacaac cggtttggac cgctgcgcgg cttgtgtgga ggggcgcgcc
      181 gttatgacat ccggaggagg cgcatggaaa accttccggc gttctccggt gcgtccaaaa
      241 ggacgcgatc accctgtggg tgcaagtctc acccgggcaa tggtcgataa actgaagtca
      301 tggacggcgc acgtccggtt cgatgagcgg ggggggacgg gtcgcatacg cggcaccgcg
      361 cccttcctcg acgctactgt tgaaagcctt gatcagcctt cgcacgaact tccccggtca
      421 accgccaaca accagacgcc cgcacgatcg gtcggcatca aacccctggt gcaagttccg
      481 tttgtggccc acgcaccagc ttacccggca gcgtcccggt cgcctgaccc tcacggaacg
      541 tcacccggcc ggaaacgatc gttgcgtcat agccgctcac cttctgcatc agccgcttgc
      601 cgcccgcagg cagatcgttg accacatacg gcgcgcccga acccagcttc ccccagtcga
      661 tgacgttcac atcggccttc ttgccgatcg ccagcacgcc acggtcatgc aaccctgctg
      721 ctccagcggt gtcggacgtc acccgtcgga tcatctccgc cacgtcccag cgcttctggt
      781 cgcggcccca gtgcgacagc atccacgtct ggtagccggc atccaggatg aacgcgacat
      841 gcgcgccgcc atcgcccagg cccatgatgc aattgcggtt gtccagcagc gtttccggca
      901 tcgacaggtt gccgtaagcg tagttcccca gaggatggta gatgaagttg gcgccgtcgt
      961 cctcgatcag catgtcgtag gcgacttcct gcggcgtccg attgccgcgc gccgcgatcg
     1021 ccgagatgct gtcctccatc ttcggctgat aattcggcgg gttgccgaag cggaacatga
     1081 agtcgaacga tatgcggtga aacagcggga aggtgttgcc ctccttcgga tcctcgctca
     1141 ggattttcgc ccgcacctcg ggatcggcca ttgtgcgagc gcgttcggcg accggcaaat
     1201 gagcgatcgt cttgtatgtc ggcgtcccgg agaacgggtt ctggctgccg gccaaaccca
     1261 gcagcacccc gatggcccga ggtgcgacga ccggccggat cggcagcccg tcacgcgcgg
     1321 ccttgtcggc atgcttgatc aggtcgcgcc acacctccgg gcgggagtgc cgctgcgtca
     1381 gcgtgaatac cgccggccgg ccgcaggcct cgatcacccg gcgcagcatg gcgaattcct
     1441 cgttatgatc cggcgcccat tccgacacca tctccagcat gcctgatccg gcatccttca
     1501 tgcccaacgc gatgccgcgc agctcttctt cctg
//
LOCUS       DASWQN010000375         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_168038, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000375 DASWQN010000000
VERSION     DASWQN010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168038"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..719
                     /locus_tag="VGC82_06135"
     CDS             9..719
                     /locus_tag="VGC82_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY0422887.1"
                     /translation="MLVPDYLTTSPSVPITPYRDGFGNWCSRLVAPAGRMRLAADGTV
                     RDSGMPDVIVPSAAQHAIEDLPPDTLVYLLGSRYCETDRLSDVAWTLFQNTTPGWPRV
                     QAICDFVHDRIAFGYEHARATMTAWEVFNEQKGVCRDYAHLAIAFCRCMNIPARYCTG
                     YLGDIGMPPPYAVGDFAGWFEAFIGGHWYTFDARNNIPRIGRVLIAQGRDAADVPITQ
                     TFGPNNLLSFKVWTDEIA"
     gene            complement(765..2051)
                     /locus_tag="VGC82_06140"
     CDS             complement(765..2051)
                     /locus_tag="VGC82_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEY0422888.1"
                     /translation="MACGSPTGGWLARLWTGPPRNRPGVDRYRGPEERISPPMSTSRP
                     EVPNVLIVEDEMVLRMRAVDIVEDAGFRPVEAVNADQAIAILESRSDISLLFTDIQMP
                     GSMDGLKLAHAVHDRWPSIKIILVSGQVTPSDAEKPADSRFFGKPLSVARMIAELQGM
                     VGAGALEILPKPIRPPVDEAAVAVPRTAKEAVLTAENDSLRLLLEQAGIDAKVLLAQA
                     GIDAKEREAADKLQKLILGELHHRIKNTLATVSAIASQSFRAASSIEDGQQAMEGRLL
                     ALGRAHDLLMKVSWASASLTHTFSGATEPYDGQGARRFHFNGPDIRITSGAVIALAMT
                     FNELCTNTTKFGALSVPDGRVEVAWTIDQDKQRLRLVWTETGGPAVRPPTRRSFGTRM
                     MESLGQQLNGQVQLAYEPSGFVYSLDVPLASIAAPA"
     gene            2257..2532
                     /locus_tag="VGC82_06145"
     CDS             2257..2532
                     /locus_tag="VGC82_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422889.1"
                     /translation="MMKSFAALATLLLLSAAVVALPAFAPPVKAEEPAALAKSDKLST
                     QPVATGCSQQVWPHFETSCLRNSGTGNRISEARLIVASPSSATNQNR"
BASE COUNT          489 a          884 c          818 g          497 t
ORIGIN      
        1 cgtccgacat cctcgtaccg gattacctga ccaccagccc ttccgtgccg atcacgccct
       61 atcgcgacgg cttcggcaat tggtgcagcc ggctcgttgc accggccggc cgcatgcgtc
      121 tggcggcaga cgggacggtc cgcgatagcg gcatgcccga cgtcatcgtt ccctcggccg
      181 cacagcacgc gatcgaggac ctcccgccgg acacgctggt ttatctgctc ggcagccgct
      241 actgtgagac cgaccggcta tccgatgtcg catggacgct gttccagaac acaacgccgg
      301 gatggccacg ggtccaggca atctgcgact tcgttcacga ccgcatcgcc ttcggctacg
      361 agcacgcgcg cgcgaccatg accgcatggg aagtgttcaa cgagcaaaaa ggcgtctgcc
      421 gcgactacgc gcaccttgcc attgcgttct gccgttgcat gaacatcccg gcgcgatatt
      481 gcaccggcta tctcggcgac atcggcatgc cgccccccta cgcggtcggg gattttgccg
      541 gctggttcga ggcctttatc ggcgggcact ggtacacgtt cgatgcccgc aacaacattc
      601 cacggatcgg ccgcgtactg atcgcccaag gccgcgatgc ggcagacgtt ccgatcaccc
      661 agaccttcgg gccgaacaat ctgctcagct tcaaggtgtg gaccgacgag atcgcgtaat
      721 cgacggcact gccgcgacga cgtcttgccg accgcacgga cgtgtcacgc aggcgctgcg
      781 atcgaggcca gcgggacatc cagcgaatag acaaacccgg acggctcgta ggccagttgc
      841 acctggccgt tcaattgctg gccgagcgac tccatcatgc gggtaccgaa gctccgccgg
      901 gttggtggcc tgaccgcggg accgcccgtt tcggtccaaa caagccgaag ccgctgcttg
      961 tcctggtcga tcgtccaggc gacttcgacg cgcccgtcgg gaaccgatag cgcgccgaat
     1021 ttcgtggtgt tggtacagag ctcgttgaac gtcatcgcga gtgcgataac ggcgccggat
     1081 gtaatccgaa tgtccggccc gttgaaatgg aagcgccggg ccccttgacc gtcatagggc
     1141 tcggtggcgc cgctgaacgt atgggtcaga ctggcgcttg cccaactgac cttcatcagc
     1201 agatcgtggg cgcgccccag cgcgagcaga cgcccttcca tggcttgctg tccgtcctct
     1261 atgctgctcg ctgcccggaa gctctgggat gcgattgcgc tcacggtggc caacgtgttc
     1321 ttgatgcggt gatgcagttc accgaggatg agtttttgca gcttgtcggc ggcctcacgc
     1381 tccttggcgt cgatgcccgc ctgcgcaagc aagaccttgg cgtcgatgcc ggcctgttcc
     1441 agcaacaggc gaaggctgtc gttctccgcg gttaaaaccg cctccttggc cgtgcggggc
     1501 acagcgacag cggcttcatc cacgggcggc cggatgggct tcggaaggat ctccaacgcg
     1561 cccgcgccta ccattccctg caattcggcg atcatccgcg cgacgctcag cggtttgccg
     1621 aagaagcggc tgtcggcagg cttttccgcg tccgacggag tcacctgacc ggaaaccagg
     1681 ataatcttga tcgagggcca gcggtcgtgc accgcatggg cgagtttgag gccgtccatg
     1741 ctgccgggca tttgaatatc cgtaaatagg agagagatgt ccgagcggga ctccagaatg
     1801 gcgatggcct gatcggcgtt gaccgcttcc acaggacgaa atccggcatc ttcaacgatg
     1861 tcgaccgcgc gcatgcgcag caccatctca tcttcgacga tcaggacgtt cggcacctcg
     1921 ggacgagatg ttgacatggg cgggctgatt ctttcttcag ggccgcgata gcgatcgacc
     1981 ccggggcggt tgcgtggcgg tcccgtccaa agccgggcca gccatcctcc ggtgggactc
     2041 ccacaggcca tccctcaacg accgctattt caatccggct gggtatgcca gcattgctgc
     2101 gcccatcggc ggcaaatagc gagctaaatc ggcgcggcgc agacgccccc tctttcagtt
     2161 cctatgttcg gaatggaacg tatcgtcgcc cggacgattg taaggcgggc tcccgaattc
     2221 ggagggggcc tgtctccgtc aacgtcagca gcgaaaatga tgaaatcctt tgcagcccta
     2281 gcgaccctcc tcctgctgag cgcggccgtc gttgccttgc cggcgttcgc tccgccggtg
     2341 aaagcagagg aacctgctgc cttggcgaaa agtgacaagc tctcgaccca accggttgca
     2401 accggctgct cgcagcaggt ctggccccat ttcgagacct cctgcctgcg taacagcggg
     2461 actggaaacc ggatcagcga agctcgcctg atcgtggcat ctccgtcttc cgcgacgaac
     2521 caaaaccgct aaagcgctac cggcgacgaa gcggggcggc gttcaatgat cgcgttgccc
     2581 ggacgccctg cccccaccag tattccctcg caaccgtgga tcgcagcgcc tgctggccat
     2641 cagaagcata cacgacgaaa ccgccggcga aggcacaggg ctcgggct
//
LOCUS       DASWQN010000376         3206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1091167, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000376 DASWQN010000000
VERSION     DASWQN010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091167"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VGC82_06150"
     CDS             complement(<1..557)
                     /locus_tag="VGC82_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0422890.1"
                     /translation="MQIGFNLPVSGPMAAADVMANIAQLGESLGFDYLTLTDHIALPD
                     TTAPGYPYSTTGAFYTPDPGHRVEQLTTAAWIAAKTSKIRLVLAVMVVPHRPAVVTSK
                     MLATIDVLSKGRLVVGIGAGWLKVELDAVATTPFAERGAVTDEYMDAMRTIWTQDKPV
                     FHGKYVNIDGLLTDPKPLQKPYPPI"
     gene            767..1657
                     /locus_tag="VGC82_06155"
     CDS             767..1657
                     /locus_tag="VGC82_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEY0422891.1"
                     /translation="MTGALILRTAFADYPHIQAIKNGSVASDRLGFDFEDVPVITRAF
                     RRMVRTLDFDLCEIALTTLAQAHAFDKPIKALPIVVMRGFHHGALICPTGSAIKAPAD
                     LRGKRIGVRAWSQTTGVWVRGILLDEYGVDHRDITWVTEEDAHVQGYVDPANVQRIAA
                     GQDLKSMLLAGDIDAGIALAGIDPVLVRPVIADPLTAAAAWFAKTGAYPVNHLLCLKT
                     SVVRAHPWLPAELMRLFTAAKAAAIEPSSEFRFQPIIRGDPLPYGVEANRTGIELCLR
                     YAAEQGLIPRPYAINEIFLE"
     gene            1732..2685
                     /locus_tag="VGC82_06160"
     CDS             1732..2685
                     /locus_tag="VGC82_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY0422892.1"
                     /translation="MTDLPKRVDIHEEGPREGFQIEPGPIATADKVRFCEALAETGLH
                     DVQCVSFVDPRRVPGMADAVEVAQSIRQRPGVHYSAIALNLRGYERAIQTKLHITGGL
                     QMSASNTFSIRNTNKDNAETLAEQRRQLEFFRDRGIPVDSAIVMTCFGCNFEGAIAPE
                     TVRDCVASLLALADEFGITLDRVRLADTVGWASPASVQRVVGLVRETWPSLRLGLHLH
                     DTRGTGLANALMGLQMGIDSFDSSCAGLGGCPFAGHKGAAGNICTEDLVFMCEEMGIS
                     TGIDLDALIECARLAEDIVGHPLPGKVMHGGSLTKYREAQV"
     gene            2682..3107
                     /locus_tag="VGC82_06165"
     CDS             2682..3107
                     /locus_tag="VGC82_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0422893.1"
                     /translation="MTEVVIEKRGMVQWITINREARRNAINDSVTDAIAGGLAEASAD
                     PDVRVVVLTGAGDKAFCAGADLSANSGSFKYDYSKVGLSFVKLMKQARDLTLPLIARV
                     NGHAMAGGLGLIGMCDMYCNGNWWKVNGANTLETNRISP"
BASE COUNT          592 a         1000 c         1015 g          599 t
ORIGIN      
        1 cagatcggcg gatagggctt ttgcaacggc ttcggatcgg tcagcaaccc gtcgatgttg
       61 acgtatttgc cgtggaagac cggcttgtcc tgtgtccaga tggtccgcat cgcgtccatg
      121 tattcatccg tcaccgcgcc ccgttcggcg aatggggtgg tcgccacggc atcgagttcg
      181 accttcagcc agccggctcc gatgccgacg accaggcgtc ctttggacag cacatcgatc
      241 gtcgccagca tcttcgacgt aaccacggcg ggccggtgcg gcacgaccat caccgccagc
      301 acgaggcgga tcttgctggt cttcgccgcg atccatgcgg ccgtggtcag ttgctccacc
      361 cgatgtccgg gatcaggggt gtagaacgcc ccggtggtgg aatagggata tcctggtgcg
      421 gtggtgtccg gcagggcgat atgatcggtc agtgtcagat agtcgaagcc cagggactcg
      481 cccagttggg caatgttggc catgacatcc gccgccgcca ttgggccgct gacgggtagg
      541 ttgaagccga tctgcatggt tcccctcgtg ttcgtcgata tccagctcga tcgcagcacg
      601 ccggcggctc gaaggcgagt caaccgaagg caccgtcctc ctgtcatgtc gccagctcgt
      661 cattgcgagc ggcgtccgtg acgtgcaaat tccggaatgg gtcatacgac ccggaggcga
      721 ctccgcgcgc cgatgctcgg tttgcctcaa tggaaggaac ggggccatga ccggtgcgct
      781 cattctgcga accgccttcg ccgactaccc gcacatccag gcgatcaaga acggttcggt
      841 tgcgtcggac cggctcggtt tcgacttcga ggacgtgcca gtcatcacca gagccttccg
      901 gcgcatggtg cgaacgctgg atttcgactt gtgcgaaatc gcgctgacca cgctggcgca
      961 ggcgcatgcg ttcgacaagc cgatcaaggc gctgccgatc gtcgtcatgc gcggctttca
     1021 ccacggcgcg ctgatctgcc cgacaggttc ggccatcaag gccccggcgg acttgcgcgg
     1081 caagcggatc ggcgtgcgcg cctggtccca aaccaccggc gtctgggtgc gaggcattct
     1141 gctggacgaa tacggcgtcg atcaccgcga catcacctgg gtgactgagg aagacgcgca
     1201 cgtccagggt tatgtcgatc cagcgaacgt gcaacgcatc gcggccggtc aggatctgaa
     1261 gtctatgctg ctggccgggg atatcgacgc cgggatcgcc ctcgccggga tcgatccggt
     1321 cctggtccgg ccggtgattg ccgatccact cacggccgcc gctgcgtggt ttgccaagac
     1381 cggggcctac ccggtcaatc acctgctgtg cctgaagact tcggtggtgc gggcccatcc
     1441 ctggctgccg gcggaactaa tgcgcctgtt caccgccgcc aaggccgcag ctatcgaacc
     1501 cagcagcgag tttcggttcc agccgattat ccgcggcgat ccgctgccct acggcgtgga
     1561 agccaaccgc accggcatcg agctgtgtct tcggtatgcc gccgaacagg gactgatacc
     1621 tcggccttat gccatcaacg agatattctt ggaatagcca agagaccgac acgccgttgg
     1681 cggaaaagcc gaggtaggtc atgatgccgg cgacatttcc gggaggaacc catgaccgat
     1741 ctgccgaaac gggtggacat ccacgaggaa ggcccgcgcg aaggctttca gatcgagccc
     1801 ggccccatcg ccaccgccga caaggtcagg ttttgtgagg cgctggcgga aaccggcctg
     1861 catgacgtgc aatgcgtttc gtttgtcgat ccacgccgcg tgcccggcat ggcggatgcg
     1921 gtggaggtcg cccaatccat ccgccagcgt cccggcgtcc actatagcgc catcgcgctg
     1981 aacctgcgcg gctatgaacg cgccatccag acgaagctgc acattaccgg cggcctgcaa
     2041 atgagcgcct cgaacacgtt ctctatccgc aacaccaaca aggacaacgc ggaaacgctg
     2101 gccgaacagc gacgccaact ggagttcttc cgggaccgcg gcattccggt ggactctgcc
     2161 atcgtcatga cctgctttgg ctgcaacttc gaaggcgcca tcgcaccgga gacagtgcgc
     2221 gactgcgtgg cttccctgct ggcgctggcc gacgagttcg gcatcacact ggaccgcgtg
     2281 cggctggcgg acaccgtcgg ctgggcatcg ccggcctccg tccagcgcgt cgtcggcttg
     2341 gtgcgcgaaa cctggccatc gctccgcctg gggctgcacc tgcacgatac ccgcggtacc
     2401 ggcctggcca acgcgctgat gggcttgcag atgggcatcg atagtttcga cagctcctgc
     2461 gccggcctcg gcggctgccc gtttgccggc cacaaagggg cggcgggcaa tatttgcacc
     2521 gaggatctcg tgttcatgtg cgaggaaatg ggcatttcga cgggcatcga cctggatgcg
     2581 ctgatcgaat gcgcgcgcct cgccgaagac atcgtcggac atcccctgcc cggcaaggtc
     2641 atgcatggcg gcagcctgac caaatatcgg gaagcgcagg tatgaccgag gtcgtgatcg
     2701 aaaagcgcgg gatggtgcaa tggatcacca tcaaccgcga ggcgcgccgg aacgcgatca
     2761 acgacagcgt caccgacgcc attgccggcg gccttgcgga ggcctcggct gacccggacg
     2821 tgcgggtcgt ggtgctgacc ggtgcaggtg ataaagcgtt ctgcgcgggt gccgatctgt
     2881 cggcgaattc aggatcgttc aagtacgact attccaaggt cggcctgtct ttcgtgaaac
     2941 tgatgaagca ggcccgggat ctgactttgc cgctgatcgc ccgggtcaat ggccatgcga
     3001 tggccggtgg cttgggcctg atcggcatgt gcgacatgta ttgtaacgga aactggtgga
     3061 aggtcaacgg cgctaacacg ttggaaacaa ataggatttc accttagtcc atagtcttac
     3121 tgcgacggcc aactgatatc gtaataaagc gcaaaccgca caaaaaagcc ccggtttccc
     3181 ggggctttcg ttatcacttt gctaga
//
LOCUS       DASWQN010000377         3141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2279495, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000377 DASWQN010000000
VERSION     DASWQN010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2279495"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VGC82_06170"
     CDS             <1..570
                     /locus_tag="VGC82_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY0422894.1"
                     /translation="AKQGVASVDAPVSGGTGGAKGATLTFMCGGDDKALAAAKPVLEK
                     MGKKIVHCGGAGAGQAAKICNNMMLAISMIGVSEAFSLAEKLGLSHQALFDVASTSSG
                     QCWALTSYCPVPGPVPASPANNGYKPGFAAALMVKDLTLAQDAAKASGAATPLGKHAQ
                     EMFRAFDAAGNGGVDFSGIIQHVRGLAGK"
     gene            581..2320
                     /locus_tag="VGC82_06175"
     CDS             581..2320
                     /locus_tag="VGC82_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0422895.1"
                     /translation="MTTFQQARAFLLRHRTDYDAAVKGFRWPDPAPFNWALDWFDAEL
                     ARNPESADRAALWIVDAGSNRETKLSFQALSRRSNQVANMLRAQGLKRGDHLLLLLGN
                     VIPLWETMLAAMKLGVVVIPATTLLTPDELRDRLDRGRARVVVASQDQVAKFAGLGGD
                     QLVRIVVAASSQQEGWLAYEQAADFPETFEPDGPTGPDDPMLLYFTSGTTAKPKLVRH
                     SQRSYPVGGLSTMYWLGLQPGDIHLNISSPGWAKHAWSCFFAPWNAGATVFVVNQPRF
                     DAKGLLATIGRCGVTTLCAPPTVWRLFIQEKLSTFKVSLREVCGAGEPLNPEVIDQVR
                     TAWGLTIRDGYGQTETTALAGNSPGQKVKIGSMGRPMPGYRVQITDGEGQVTKEGEVT
                     LLLAPDRPAGLMQGYQGEDGKLSGADGDLYRSGDVVFADDEGYLTFVGRSDDVFKSSD
                     YRISPFELESVLLEHELVAEAAVVPSPDPIRLAIPKAYVLLVAGAARSPETALSIFRH
                     LHTRLAPFKRIRRLELVTELPKTISGKIRRVQLRRLEHDNNRDDALRGKEFREEDFPE
                     LQKVRSAGATENG"
     gene            2321..2800
                     /locus_tag="VGC82_06180"
     CDS             2321..2800
                     /locus_tag="VGC82_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY0422896.1"
                     /translation="MNEVWKKPPISLDAYQKMVGHEVGVSSWHVVDQNRINAYADVIE
                     DHQFIHIDPERAKKETPFGTTVAHGFLTMSLLSIMSYEVMPVIQGTTMGVNYGFDRLR
                     FISPVRSGSRVRGRFRLAEAKLRKPTELLSRTNVSVEIEGEEKPALVADWLGLIYFA"
     gene            2924..>3141
                     /locus_tag="VGC82_06185"
     CDS             2924..>3141
                     /locus_tag="VGC82_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEY0422897.1"
                     /translation="MTIRFDGRVAIVTGAGNGLGRAHALGLASRGARVVVNDFGGARD
                     GSGGSLSPAEAVVEEIRRAGGVAMADGA"
BASE COUNT          566 a          974 c         1058 g          543 t
ORIGIN      
        1 gcgaagcagg gcgtcgcctc cgtggatgcg ccggtctccg gcggcaccgg cggcgccaag
       61 ggcgcgacgc tgaccttcat gtgcggcggc gacgacaagg ctttggcggc ggcgaagccg
      121 gtgctggaaa agatgggcaa gaagatcgtg cattgcggcg gcgcgggcgc ggggcaggcg
      181 gccaagatct gcaacaacat gatgctcgct atttccatga tcggggtcag cgaggccttt
      241 tcgcttgccg aaaagctggg cttgtcgcac caggcgctgt tcgatgtggc ctcgacctcg
      301 tcgggacaat gctgggcgct gacctcctat tgtccggtgc cgggcccggt gccggcttcg
      361 cccgcgaata acggctacaa gcccggcttt gcggccgccc tgatggtaaa ggacctcacg
      421 ctggcgcagg acgcggccaa ggcctctggc gcggcaacgc cgcttggcaa gcacgcgcag
      481 gaaatgttca gggcgtttga cgccgcggga aatggcgggg tcgatttctc agggatcatc
      541 cagcacgtcc gcggcctggc cggaaaatag ctgaagaccg atgaccacat tccagcaagc
      601 ccgcgcgttc ctgctcaggc atcgcaccga ttacgatgcc gcggtgaagg gattccgctg
      661 gccggatccg gcgccgttca actgggcgct ggactggttc gacgcggaac tggcgcgcaa
      721 cccagaaagc gcggatcgcg cggcgctgtg gatcgtcgat gccggcagta accgggagac
      781 aaagctctcg ttccaggcgc tgtcgcggcg ttccaatcag gtcgccaaca tgctgcgcgc
      841 gcaagggcta aagcgcggcg atcatctgct cttgctgctc ggcaacgtca ttccgctgtg
      901 ggagaccatg ctggcggcga tgaagctcgg cgtggtggtg attcccgcca ccacgctgtt
      961 gaccccggac gaactgcgcg accggctcga tcgcgggcgt gccagggtgg tggtggcgtc
     1021 gcaggaccag gtcgcgaaat ttgccggcct cggcggcgat caactggtcc gcatcgtcgt
     1081 cgccgcatcc tcgcaacagg agggctggct tgcctacgag caggccgcgg atttcccgga
     1141 gacctttgag ccggatggac cgaccgggcc cgacgatccg atgttgctgt atttcacctc
     1201 cggcaccacg gcaaaaccaa agctggtgcg gcacagccag cgcagctacc ccgtcggcgg
     1261 attgtcgaca atgtactggc tcgggctgca gcccggcgac atccacctca acatttcctc
     1321 gccgggctgg gccaagcacg cctggagctg cttcttcgcg ccctggaatg ccggcgccac
     1381 ggtgttcgtg gtcaaccagc cgcgcttcga cgccaaggga ttgctcgcca ccatcggccg
     1441 ctgcggcgtc accacgctgt gcgcgccgcc gacggtgtgg cggttgttca tccaggaaaa
     1501 gctttcgacc ttcaaggtca gcttgcgcga ggtgtgcggc gccggcgagc cgttgaaccc
     1561 ggaagtgatc gatcaggtgc gcacggcctg ggggctgacc atccgcgacg gttatggcca
     1621 gaccgaaacc acggcgctgg ccggaaactc gccggggcaa aaggtgaaga tcggatcgat
     1681 gggtcggccc atgcccggtt atcgcgttca gatcaccgac ggcgaggggc aggtaacgaa
     1741 ggagggcgag gtcacgctgc tgctcgcccc ggacaggccg gcgggcctga tgcagggcta
     1801 tcagggcgaa gacggcaaac tgagcggcgc cgacggcgat ctctaccgca gcggtgacgt
     1861 ggtgtttgcc gacgatgagg gctatctgac cttcgtcggc cgctccgatg acgtcttcaa
     1921 atcatccgac taccggatca gcccgtttga actcgagagc gtcctgctcg agcacgagct
     1981 cgtggcggaa gcggcggtcg ttcccagccc cgatccgatc cggctcgcca tccccaaggc
     2041 ctatgtgctg ctggtcgcgg gcgcggcgcg ttcgcctgag acggcgctgt cgatcttccg
     2101 gcatctgcac acaaggctgg cgccattcaa gcgcatccgg cggcttgaac ttgtaaccga
     2161 gctgccaaag acgatttccg gcaaaatccg ccgtgtgcag ttgcgccggc tggagcacga
     2221 caacaaccgt gacgacgcgt tgcgcgggaa agaattccgc gaagaggact ttccggagtt
     2281 gcagaaagtg cggtccgccg gtgcgacgga gaacggatga atgaacgaag tctggaagaa
     2341 gccgcccatc tcattagacg cctaccagaa gatggtcggc cacgaagtcg gcgtgtcgtc
     2401 ctggcatgtg gtcgatcaga acaggatcaa cgcctatgcc gatgtgatcg aggatcacca
     2461 gttcatccat atcgaccccg agcgcgccaa aaaggaaacc ccgttcggca ccacggtggc
     2521 gcatggcttc ctgacgatgt cgctgttgag catcatgtcc tacgaggtga tgccggtgat
     2581 ccagggcacg acgatgggcg tcaattacgg gtttgacagg ctgcgcttca tctcgccggt
     2641 gcgttccggt tctcgggtcc gcggccgctt caggctagcg gaggcgaagc tgcgcaagcc
     2701 gaccgaactg ctgtcgcgca ccaatgtcag cgtcgagatc gagggcgagg aaaagcccgc
     2761 cctggtcgcc gactggctcg ggctgatcta tttcgcgtga gccccacccg tcattgccgg
     2821 gcttgacccg gcaatccatc gctggtcaaa accgctccgc tatttctctt gatggatgcc
     2881 cggatcaagt ccgggcatga caaccaaatc cgggatttaa tgcatgacaa tcaggtttga
     2941 cggacgcgtc gctatcgtta ccggcgcggg caatggcctt ggacgcgcgc atgcgctggg
     3001 cctggcgagc cgcggcgcca gggtcgtcgt caatgatttc ggcggcgccc gcgacggcag
     3061 cgggggatcg ctgtcccctg cggaagccgt ggtcgaggaa atccgcagag ctggcggcgt
     3121 ggcgatggcc gacggcgccg a
//
LOCUS       DASWQN010000378         5571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2410978, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000378 DASWQN010000000
VERSION     DASWQN010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2410978"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /gene="phoU"
                     /locus_tag="VGC82_06190"
     CDS             complement(<1..707)
                     /gene="phoU"
                     /locus_tag="VGC82_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY0422898.1"
                     /translation="MSDATEHLVKSFDNDLRRLRDMLTEMGGIVESQVAMAAEAIMNR
                     DSAAAMRAVEEDPKVDALERDVEQFVIRMLALRQPMAGDLRRVVAALKITGDLERIGD
                     YAANVAKRSIVLGQYTLPYSLAGLAHMTTLVQEQLKSIMDALGTGDAAKAIEVWRSDR
                     VVDDIYNALFRELITYMMEDPRNITPCTHLLFIAKNLERIGDHATNIAETVYYAVEGE
                     VISDARPKGDSSSYTIVR"
     gene            complement(727..1485)
                     /gene="pstB"
                     /locus_tag="VGC82_06195"
     CDS             complement(727..1485)
                     /gene="pstB"
                     /locus_tag="VGC82_06195"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY0422899.1"
                     /translation="MNPARIAISDLDFFYGDHRALKGIRLNLPERQVTGMIGPSGCGK
                     STLLRVLNRMYDLYPGQRATGQVLMGETNIIDPKLDLNALRARIGMVFQKPTPFPMTI
                     FDNIAFGVKLHERLSKSAMDERVEWSLTRAALWNEVKDRLQTSAMGLSGGQQQRLCIA
                     RTIAVRPEVILLDEPTSALDPISTLKIEELIDELKHDFTIAIVTHNMQQAARCADQVA
                     FFYLGEMVECAPAVQIFTSPKQKRTQEYVTGRFG"
     gene            complement(1568..2452)
                     /gene="pstA"
                     /locus_tag="VGC82_06200"
     CDS             complement(1568..2452)
                     /gene="pstA"
                     /locus_tag="VGC82_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY0422900.1"
                     /translation="MSATTAYPWPPRDPAASRRLGRRRYGTNTLVKLCCVLATTIGLA
                     LLASILLTLLWRGVDGLALSVFTTSTLPPGSHGGLLNAIIGSLIQTFLGAAIGTPIGL
                     MVGTYLAEYAQGSMIGNAVRFVSDVLLSAPSILIGLFVYQIAVSPFGGFSGFAGCLAL
                     AVIVIPIVVRTTEDMLRLIPSTLREAVIALGAPKWKMIVLVCYRAAKEGIATGILLAI
                     ARIAGETAPLLFTSLGNLNWSLSLGKPMASLPVTIYQYAGSAFEDWVQLAWVAALLIT
                     FGVLAINIVARVLLHRAK"
     gene            complement(2449..3411)
                     /gene="pstC"
                     /locus_tag="VGC82_06205"
     CDS             complement(2449..3411)
                     /gene="pstC"
                     /locus_tag="VGC82_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY0422901.1"
                     /translation="MLIVPQAVTADQERSKARPGDAIFAVLARSSGLFVLLLLGAIIV
                     VLFLGGLKTFRTFGAGFLVSDDWDPVQEVYGAAVPIFGTVVTAVLALTLAVPMAFGIA
                     FYLTEIAPGWVRRPVSTAIELLAAVPSIIYGMWGFFVIVPIMAQYVQPTLIDNLGDIP
                     LIGILFQGPPFGTGIFTAALILAVMIIPFIAATMRDVFDTVPPVFKESAYGLGCTTWE
                     VMRSIVIPYTRVSVVGGIMLGLGRALGETMAVTFVIGNTNRITSSLFGPGNTIASLVA
                     LEFPESQSGSLKLSSLLALGFILFVISFIVLATSRFLLRPRLKA"
     gene            complement(3467..4507)
                     /gene="pstS"
                     /locus_tag="VGC82_06210"
     CDS             complement(3467..4507)
                     /gene="pstS"
                     /locus_tag="VGC82_06210"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY0422902.1"
                     /translation="MKIAMAAVAFGFAGFAATMNQAEAQQITGAGATFPAPVYTKWGE
                     QAKAALGIELNYQAIGSGGGQNQILQRTVDFGASDAPMPPEKLESGKLLQFPTVMGSV
                     VVIVNVAGVETNQLKLTGDLLAAIYQGKITKWNDPKLVEANGGVTLPNLAIAPVYRAD
                     GSGTTYVWTSYLSAVSSDWKEKVGANTSVKWPAGAGAKGNDGVAATVHNTRGGIGYVE
                     YAFASQNHLVTVQLRNKAGVFVKPTMEAFQAAAANGDWANAKNFAVNLIDQPGEKSWP
                     IESATFIELPKDPADPARSAAVMKFFDWAYKNGDASAISLEYVPLPDAVKTAVRSAWH
                     ANIMGPDGKPIN"
     gene            4679..5125
                     /gene="dut"
                     /locus_tag="VGC82_06215"
     CDS             4679..5125
                     /gene="dut"
                     /locus_tag="VGC82_06215"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY0422903.1"
                     /translation="MSIPILIRRLPHADGLALPSYATAGAAGMDLLAAILQPVTVGPG
                     ERALIPTGLTIALPAGYELQIRPRSGLALRYGITLPNAPGTIDEDYRGEIAVIVLNTG
                     TEAFVVERGARIAQAVLAPVSRADWREVDSLDVTERNAAGFGSTGT"
     gene            5251..5454
                     /locus_tag="VGC82_06220"
     CDS             5251..5454
                     /locus_tag="VGC82_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422904.1"
                     /translation="MLADTAEVELVAASVVDGSNPATDGPMASVQVEVAERPMAAARG
                     ILTPVLMSIPIWMALALVVYMFR"
BASE COUNT         1078 a         1728 c         1727 g         1038 t
ORIGIN      
        1 cgaacgatag tataagacga tgaatccccc ttgggacgcg catcggagat gacctcaccc
       61 tcgacagcgt agtaaacggt ctcggcgatg ttcgtcgcgt ggtcgccgat ccgttccaaa
      121 ttcttggcga taaacagcag atgggtgcag ggcgtgatgt tgcgcggatc ttccatcatg
      181 taggtgatca actcgcggaa cagcgcgttg tagatgtcgt ccacgacgcg gtcggaccgc
      241 cagacctcaa tcgccttggc ggcatcgccg gtgcccagcg cgtccatgat cgacttcaac
      301 tgctcctgca ccagcgttgt catgtgggcg aggccggcca gcgaatacgg cagggtgtac
      361 tgtcccagaa cgatgctgcg cttagccaca ttagcggcgt agtcgccgat ccgctccaga
      421 tcgccggtga tcttcaaggc ggcgacgaca cgccgcaggt cgcccgccat cggctggcgg
      481 agcgccaaca tgcggatgac gaactgctcc acgtcgcgct ccagcgcatc gaccttgggg
      541 tcctcttcca ccgcccgcat tgccgccgcg ctgtcacggt tcatgatcgc ttcagccgcc
      601 attgcgacct ggctttcaac gataccgccc atctcggtca gcatgtcgcg caggcgtcgc
      661 aggtcgttgt cgaagctttt gaccaggtgt tcggtcgcat cggacatcgt gatgggcctt
      721 tccttctcag ccgaaccggc cggtgacgta ttcctgggtg cgcttctgct tcggcgaagt
      781 aaatatttgt accgccggcg cgcattccac catttcgccc aagtagaaaa atgccacctg
      841 atcggcacaa cgcgccgcct gctgcatgtt gtgagtaacg atcgcgatgg tgaaatcgtg
      901 cttcaactcg tcgatcagtt cctcgatctt cagagtgctg atgggatcga gcgcgctggt
      961 cggttcgtcc aacaggatga cctccggccg cactgcgatc gtgcgcgcga tgcaaaggcg
     1021 ctgctgctgg ccgccggaca gccccatggc ggatgtctgc agccggtcct tgacctcgtt
     1081 ccatagtgcg gcgcgggtca gcgaccactc cacccgctca tccatcgccg acttcgacag
     1141 ccgttcatgc agctttacgc caaaggcgat gttgtcgaaa atcgtcatcg ggaacggagt
     1201 cggcttctgg aacaccatcc cgatgcgggc gcgcagtgcg ttcaggtcga gtttgggatc
     1261 gatgatgttt gtctcgccca tcaacacctg gccggtcgca cgctggccgg gatacaggtc
     1321 gtacatccgg ttcagcacac gcagaagagt ggacttgccg cacccggacg gaccgatcat
     1381 gccggtaacc tgacgttccg gtagattcag cctgatgccc ttcagcgcgc ggtgatcgcc
     1441 atagaagaaa tcgaggtcgc tgatcgcgat acgcgccggg ttcatcgacg ccgccgcacg
     1501 ggcctgcacg ccgccgaagc tggcgttcgc agttccactg gaagttgcgg tcgcactcat
     1561 gttcacctca ttttgcgcgg tgcagcagca cccgagccac gatgttgata gccagaacgc
     1621 cgaacgtgat gagcaaggcg gcgacccagg cgagttgcac ccagtcctcg aacgccgatc
     1681 cggcatattg atagatggtg acgggcaggc tggccatcgg cttgccgagg ctcaatgacc
     1741 agttcaggtt gcccaggctg gtgaacagca gcggtgccgt ttcaccggcg atgcgcgcga
     1801 tcgccagcag gatgcccgtg gcgatgcctt cctttgctgc ccggtagcag accagcacga
     1861 tcatcttcca tttcggcgcg cccagggcga tgaccgcttc acgcaaagta gagggaatca
     1921 gccgcagcat gtcctcggtg gtgcggacga cgatcgggat cacgatcacc gccagcgcca
     1981 gacagccggc gaaaccgctg aatccaccga acggggaaac cgcgatctgg tagacgaaca
     2041 gtccaatcag gatcgacggc gccgacaaca gcacgtccga gacgaaccgc accgcgttgc
     2101 cgatcatcga tccttgggca tattcagcca gataggtgcc aaccatcagc ccgatcggcg
     2161 taccgatagc ggcgcccaga aaggtctgga tcaagctgcc gatgatcgca ttcagcaaac
     2221 cgccatgcga gccgggcggc aaagtggagg tcgtaaaaac cgatagtgcc agcccgtcca
     2281 cgccgcgcca cagcaaggtg agcaggatcg acgccaggag cgccagcccg atggtcgtcg
     2341 ccaggacgca gcacagcttg accagggtgt tggtcccgta ccggcggcgc ccgaggcggc
     2401 gtgatgcggc tggatcccgg ggaggccaag gataggcggt cgtcgcgctc atgccttcaa
     2461 cctcggccgc agcagaaacc ttgaagttgc caacacgatg aaggagatga cgaacaggat
     2521 gaagcccagc gccagcagcg aggaaagttt caggctgcct gactggcttt cgggaaactc
     2581 cagcgcgaca agcgaggcga tggtgttgcc gggcccaaac agcgatgacg tgatccggtt
     2641 ggtattaccg atcacgaatg tcaccgccat cgtctccccc aatgctcggc cgagtcccag
     2701 catgatgccg ccgacgaccg agacgcgggt gtaggggatg acgatcgagc gcatcacctc
     2761 ccatgtcgtg cagcccaggc cgtaagcgga ttccttgaat accggcggca cggtgtcgaa
     2821 cacatcgcgc atggtcgcgg cgatgaaggg aatgatcatc accgcgagga tcagcgccgc
     2881 ggtgaagatg ccggtgccga acggcggccc ctgaaacagg atgccgatca gcgggatgtc
     2941 gccgagattg tcgatcagcg tgggctggac gtattgcgcc atgatcggga cgatcacgaa
     3001 gaagccccac atgccgtaga tgatcgacgg caccgcggcg agcaactcga ttgccgtgct
     3061 gaccggccgg cggacccagc cgggggcgat ctccgtcagg tagaaagcga tgccgaaagc
     3121 catcggcaca gccagggtca gggccagcac ggcggttacc acggtgccga agattggcac
     3181 cgccgcgcca taaacctcct gaaccgggtc ccagtcgtcg gataccagga agcccgcgcc
     3241 gaacgtccgg aaggtcttga gcccgcccag gaacaggacc acgatgatgg cgcccaggag
     3301 caacaggacg aacaggcccg aggatcgcgc caggacagcg aagatggcgt cacccgggcg
     3361 ggctttggat cgctcttggt cggcggtgac agcctgcggc acgatcagca tcctaaatcg
     3421 gcgatacaag cgaaggacgg gacaagcgcc agtcgcttcc gtaggcttaa ttgatcggtt
     3481 tgccgtcggg gcccatgatg ttggcgtgcc aggcgctgcg cacggccgtc ttcacggcat
     3541 ccggcagcgg cacgtattcc agtgagatcg ccgaggcatc gccattcttg tacgcccagt
     3601 cgaagaactt catcactgcg gccgaacggg ctggatcggc cgggtccttg ggcagttcga
     3661 tgaacgtggc ggactcgatc ggccagcttt tttcacccgg ctggtcgatc agattgaccg
     3721 cgaaattctt cgcgttcgcc cagtccccgt tcgcggcggc cgcttggaag gcttccatcg
     3781 tcggcttgac gaacacgccg gccttgttgc gaagctgcac ggtcaccagg tggttctggc
     3841 tggcaaaggc atactcgaca tagccgatgc cgccgcgcgt gttgtggacc gtggcggcaa
     3901 cgccgtcatt gcccttggcg ccggcgcccg cgggccattt cacgctggtg ttggcaccga
     3961 ccttttcctt ccagtcggag ctgaccgcgg acaggtagga ggtccagaca taggtcgttc
     4021 ccgaaccgtc ggcacggtaa accggcgcaa ttgccaagtt cggcagggtg acgccgccgt
     4081 ttgcctcgac cagcttcgga tcattccatt tggtgatctt accttgatag atcgcggcca
     4141 gcaggtcacc cgtcagcttg agctgattgg tttccacgcc ggcgacattg acgatcacga
     4201 cgaccgaacc catcaccgtc ggaaactgca gcaacttgcc ggactcaagt ttttccgggg
     4261 gcatcggcgc gtcggaggca ccgaaatcga ccgtgcgctg caggatctgg ttctgtccgc
     4321 cacctgaccc tattgcctgg taatttagtt cgatgcccaa tgcggccttc gcctgctcac
     4381 cccatttcgt atagacgggc gctgggaagg tcgctcccgc gccggtaatc tgctgggctt
     4441 cggcctgatt catggtggcg gcaaatccgg caaagccaaa ggccacagcc gccatggcga
     4501 tcttcatcaa tttgactcct tcggggccca cgcgcattgg cgtccgggaa ccgtaggtcg
     4561 caccggtgaa gcccgtattg cagttttatg acagttgcgt gacagcgcca ggctggctgg
     4621 accgctggtg acggcgttgg cttgcggggc aaccgcgcgg cacggcacaa tcttctttat
     4681 gtcgattccc atccttattc gccgtttgcc gcatgccgac gggctagccc tcccgtccta
     4741 cgcaaccgcc ggggctgccg gaatggatct tctcgcggcc atcctgcaac cggttaccgt
     4801 ggggccgggg gaacgggccc tgattcccac aggcctgaca atcgcgctgc ctgctggtta
     4861 cgagttgcag atccggccac gttccggcct tgccttgcgt tacggcatca ccctgccgaa
     4921 cgccccggga acaatcgatg aggactaccg cggggaaatc gcggttatcg tcctcaatac
     4981 cggcaccgaa gcctttgtgg tcgagcgcgg cgcccggatc gcccaggcgg tgctcgcgcc
     5041 cgtcagccgg gcagattggc gggaagtgga cagcctggac gtgacggagc gcaacgcggc
     5101 cggtttcggc agcactggaa cctgacgtcg aggtcgggac gttctgatct tcggatagtg
     5161 atcgctcaac tagttgtttg caatctcatt gcaatatggt aaatatgacc aggccggaag
     5221 aaaacacatc tccgcccggg aacaaaagtg atgcttgcag acacggccga ggtcgaactg
     5281 gttgccgctt ccgtggtcga tggcagcaac ccggcaacgg atgggcccat ggcatccgtc
     5341 caggtcgagg ttgccgaacg gccaatggca gctgccagag gtattcttac cccggttctg
     5401 atgtcgatcc caatctggat ggccctggcg ctggtggtct atatgttcag gtaagagatc
     5461 agccttgcgc cggcacccac acttttcccg gttaggacaa gtcgtgtgtg gccgcgcctg
     5521 tcaggcacgc tcttgagtcc ggatgcgata acctgcggta caacgtgcgg c
//
LOCUS       DASWQN010000379         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_564382, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000379 DASWQN010000000
VERSION     DASWQN010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564382"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VGC82_06225"
     CDS             complement(<1..758)
                     /locus_tag="VGC82_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEY0422905.1"
                     /translation="MRPYQIGLLAALGIVAVGGGWYFGTGSTPAEQVAGGQLMFPDLA
                     PRLRDAVKIEILHQGKQTVIERRPNGGWGIASMHGYPVQNAKLNGLFAGLTELRLTEP
                     RTTDPAMFARLGVDDPNVAASTANLLRVLDASGKPILAVIVGHRRVRSQANAPEDVYV
                     RRPDDNQSWLAEGTLQVDADASAWLDRDVINIGHDRVATVVAGDHALTFARVDGKFGL
                     SQPAEHPKLEDYKIDDVARGLETLTLQQVRADAD"
     gene            complement(755..2671)
                     /locus_tag="VGC82_06230"
     CDS             complement(755..2671)
                     /locus_tag="VGC82_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEY0422906.1"
                     /translation="MRRLWFSIVGVIAVAAIAIGVNMFADARLANVQLDLTQGRLYTL
                     SAGTRQVLADLKEPVTVRLFYSRQLGSTVPAYGTYADHVREMLRDYAAISGGKVKLEF
                     YDPEPFSDIEDRAMAYGLQGVPLDQGGSQVYFGLVGTNLEDDERTVPFFQAERERFLE
                     YDLTKLVYDLSNPTRPVVGVMSSLPLDGDPRAMMMSQGRGSGGQPYASSTLLRQTNTV
                     KTVALDAQVIDPDVTVLLVAEGQNLSPATLYAIDQFVMRGGKLMAMVDPWSEALAAVP
                     SQTGMPPSDTHADLKTLFDAWGIVFDPSKVVGDLTGAWRVRTGGNDRVQAVNYVAWFN
                     IRDGLNHDDPATADLQQVTVASAGALSKAPNAAIDFTPLLTSSDHSGLIAVDAVKVPE
                     PAKVLAGFKPEGGSRVIAARVRGVLKSAFSGPPPLPVGGEQKRPDGFPPHRAQTDGPA
                     NLVVVADSDILADRFWVRVADFFGQQTATPFADNGPFVANLVGTLAGSDALIGLRSRG
                     GTNRPFTLVTAMQSDAEARFRQTQQTLQAHLDDTEKQLRTLRSGGGDTADKARPESVI
                     TPEQHAAIEAARQEVLQTRQKLRAVQLELNRDISALANEMRAFTILLVPGLLAVLAIG
                     MGVVQRRRRARARS"
     gene            complement(2676..>3265)
                     /locus_tag="VGC82_06235"
     CDS             complement(2676..>3265)
                     /locus_tag="VGC82_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0422907.1"
                     /translation="DLNPFFTFIPWVFLLLIPAITMRLWAEERRLGTIELLLTLPITQ
                     GQAVVGKFLAAWAFCAIALVLTFPFVIAVNILGSPDNGVIASGYIGCLLIAGAFLSVG
                     AAMSAVTRNQVIAFVLSVAICFLFSVASNPIVNDFLSHNIPVLGAFTRRIAVIDRFND
                     FTRGVIALRDLVYFAAFIGFFLFLNTVLVDQRKAD"
BASE COUNT          631 a         1105 c          977 g          552 t
ORIGIN      
        1 ctatcggcat cggcacgaac ctgctgcaaa gtcagcgtct ccagtcctcg cgccacatcg
       61 tcgattttgt agtcctccag tttgggatgt tcggctggct ggctcagccc gaacttgccg
      121 tccacccgtg cgaacgtcag cgcgtgatcg ccggcgacca ccgttgcgac gcggtcatgg
      181 ccgatattga tgacatcgcg gtcgagccac gccgaggcat ccgcatcgac ctgcaaagtg
      241 ccctccgcca gccaggactg gttatcgtcc ggccggcgga cataaacgtc ctccggcgca
      301 ttggcctggc tgcgcacccg ccggtgcccg acgatgaccg ccaggatcgg ttttcccgag
      361 gcatccagaa cgcgcagcag attcgccgtg gatgccgcga cattcggatc gtccaccccc
      421 aaccgggcga acatggctgg atcggtggtc cgcggttccg tcagccgcaa ttccgtcagt
      481 cccgcgaaca gaccgttcag cttggcgttc tgcaccgggt aaccgtgcat ggaggcgatt
      541 ccccagccac cattcggccg cctttcgatg acggtctgct tgccctggtg caagatctcg
      601 atcttgaccg catcccgcag tcgcggcgcc aggtctggaa acatcaactg cccgcctgcg
      661 acctgttccg cgggtgtgga gccggtgccg aaataccagc cgccgcccac tgccacgatg
      721 cccagcgctg ccagcagccc gatttggtaa ggtctcatga tcgcgccctc gcacgccgcc
      781 gccgctgcac cacacccatg ccgatcgcca gcaccgccag cagtccggga acgagcagga
      841 tggtgaacgc gcgcatctcg ttcgccagcg ccgagatatc gcggttcagc tccaactgca
      901 ccgcccgcag tttttgccgt gtctgcagca cctcctgccg cgccgcctca atcgccgcat
      961 gctgctcggg cgtgatgacc gactcgggcc tggccttgtc cgccgtgtcc ccaccacccg
     1021 accgcagggt gcggagctgt ttctcggtgt cgtcgagatg cgcctgcaaa gtctgctgag
     1081 tttggcgaaa ccgagcttcg gcatcgcttt gcatggctgt aaccagcgtg aacggccggt
     1141 tggtgccgcc acgggaacgc aagccgatca gcgcgtcgct gccggccaac gtgccaacaa
     1201 ggttggccac gaacgggccg ttgtcggcga acggcgtggc ggtctgttgg ccgaagaaat
     1261 cggcgacccg cacccagaac cggtcggcca ggatgtcgga gtcagcgacc accacaaggt
     1321 ttgccggccc atcggtctgc gccctgtggg gcggaaatcc gtccggccgt ttctgttcgc
     1381 cgccgacggg cagcgggggt ggtccggaga acgccgactt caatacccca cgcaccctgg
     1441 cggcgatgac gcgcgatccg ccttcgggct tgaagccagc cagtactttg gccggctccg
     1501 gcaccttcac ggcgtcaacc gcaatcaacc cgctatggtc gctggatgtc agcaaagggg
     1561 tgaagtcaat cgcggcattc ggtgccttcg acagggcacc agcggacgcg accgtgacct
     1621 gctgcagatc ggcggtggcc gggtcgtcat ggttcagccc atcgcggata ttgaaccagg
     1681 cgacgtagtt taccgcctgc acccgatcat tgccgcccgt ccgcacgcgc cacgcgccgg
     1741 tcaaatcgcc caccaccttc gacgggtcga acacgatccc ccaggcgtca aacaacgttt
     1801 tcaggtccgc atgggtatcc gacggcggca tcccggtctg tgagggcaca gccgccagcg
     1861 cttcgctcca tggatcgacc atcgccatca gcttgccgcc gcgcatcacg aactggtcga
     1921 tggcatacaa cgtcgccggc gacaggttct gcccctcggc caccagcagc accgtgacgt
     1981 ccggatcgat tacctgagcg tccaacgcca cggttttcac cgtgttggtc tgccgcaaca
     2041 gggtggacga tgcgtatggc tggccacccg aaccacgtcc ctgggacatc atcatcgccc
     2101 tcggatcgcc atccagcggc aatgaggaca tcacgccgac caccggacgc gtcgggttcg
     2161 acaagtcata aaccagcttg gtcaggtcat attccaggaa ccgttcccgc tccgcctgaa
     2221 aaaacggaac ggtgcgctcg tcgtcttcaa ggttggtgcc gacaaggccg aagtaaacct
     2281 gcgacccacc ctggtccaac ggcacccctt gcagcccata cgccatcgcc cggtcctcga
     2341 tatcgctgaa cggctccggg tcgtagaact ccagctttac tttgccgccg gaaatcgcgg
     2401 catagtcgcg cagcatctcc cgcacatggt cggcataggt gccgtaggca gggaccgtgc
     2461 tgccgagttg ccgcgaatag aacagccgca ccgtcaccgg ctctttcaga tcggccagca
     2521 cctgccgggt tccagccgac agcgtgtaaa ggcgtccctg cgtcagatcg agttgcacgt
     2581 tcgcaaggcg cgcatcggca aacatgttga cgccaatggc gatcgccgcg acggcgatga
     2641 cgcctacaat agaaaaccac agccggcgca tgatctcagt ccgccttccg ttgatcgacc
     2701 agaaccgtgt tcaggaacag gaaaaacccg ataaaggctg cgaagtaaac caggtcccgc
     2761 agcgcaatga cgccccgggt gaagtcgttg aaacggtcga tgaccgcaat ccgccgggtg
     2821 aacgccccca gcaccgggat gttgtgcgac aggaagtcgt tgacgatcgg gttggaggcg
     2881 acgctaaaca ggaaacagat cgccaccgac agcacgaatg cgatcacctg gttgcgggtc
     2941 accgccgaca tcgcggcacc taccgacagg aacgcgcccg caatcaacag gcagccgata
     3001 tagcccgacg cgatgacccc attgtcgggc gatccaagga tgttcaccgc gatcacgaac
     3061 gggaaggtca acaccagcgc gatggcgcaa aacgcccagg cggcaaggaa ctttccgacc
     3121 actgcctgac cctgggtgat cggcagggtc agcagcagtt cgatggtgcc gagccggcgt
     3181 tcctcggccc agagccgcat ggtgatggcg gggatcagca gcaggaacac ccacgggatg
     3241 aaggtgaaga acggattgag gtcag
//
LOCUS       DASWQN010000380         3139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2279822, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000380 DASWQN010000000
VERSION     DASWQN010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2279822"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VGC82_06240"
     CDS             <1..760
                     /locus_tag="VGC82_06240"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0422908.1"
                     /translation="KAIEEALALVELSAYADRPVTQLSGGQMQRVALARSMVYQPQIL
                     LLDEPLSNLDAQLRLRLRDDLRRIIKRVGLTALYVTHDQQEAVVLGDRIGVMRDGLLL
                     QLAAPDELYNRPADLFVAGFTGASNVIAGRLQSRDGGFGAVSVGAGNSLIVRLAGDAA
                     PGSALNVALRPDNVLLEPANDGPNQFPGRVVSRHYQGTQTVYEVSLFGQTIEALEVGS
                     SIRHAVNDAVTIALPAEVCWGYAAAVDHRLRGSG"
     gene            complement(972..1976)
                     /locus_tag="VGC82_06245"
     CDS             complement(972..1976)
                     /locus_tag="VGC82_06245"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY0422909.1"
                     /translation="MSKFGRRTFVGTALTAPAFGIVCARAAEFVFKYGNNVPETYPLN
                     VRISQAAERIRAATNGRFDLQIFPAGQLGTDTDMLSQVRSGAIDFYTASGLVLSTLVP
                     LTAINALGFAFRDYTQVWPAMDGKLGTAIRARIDAAGLHAIDKIFDIGFREVSSSTHP
                     ITAPDSFKGFKIRIPPSQLGVSMFRALGAAPATLNFAEAYTALQTHVVDGQENPLSII
                     DTAKFSEVQKYVSMTNHMWDGYWLLCNGRAWKGLPDDIRSVVSRELNGAAEEDRVDIA
                     ALDASLKGKLESQGMTFNTPDPEPFRTILRRDGFYDQWRKTFGDEAWALMEAYVGRLS
                     "
     gene            complement(2039..>3139)
                     /locus_tag="VGC82_06250"
     CDS             complement(2039..>3139)
                     /locus_tag="VGC82_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0422910.1"
                     /translation="VVGVLVGDTGHVLRIRREIFVIIDVHGHYTTAPKALEVWRNRQI
                     AGIGDKSTMPKVSDLKISDDELRESIIGNQLAKMQDRGIDLTIFSPRASFMAHHIGDF
                     NVSATWAAICNELCYRVSQLFPGHFIGAAMLPQSPGVDPRTCISEMEKCIKEYGFVGI
                     NLNPDPSGGHWTSPPLSDRQWYPIYEKMVEYDVPAMVHVSTSCNDCFHTTGAHYLNAD
                     TTAFMQCLTSDLFKDFPTLKFLIPHGGGAVPYHWGRFRGLAQEMKKPLLTELLLNNIY
                     FDTCVYHQPGIDLLTKVIPVKNILFASEMIGAVRGIDPETGFYYDDTKRYIEASTILS
                     PEAKEAVYEGNARRVFSRLDAALRADTTSVAS"
BASE COUNT          619 a          938 c          942 g          640 t
ORIGIN      
        1 gaaggcgatc gaggaagcat tggcgctggt cgaactgtcc gcctacgccg atcgcccggt
       61 gacgcagttg tccggcggcc agatgcagcg tgtcgcgttg gcccgcagca tggtctatca
      121 gccgcaaatc ctgctgctgg acgaaccgct gtccaatctc gatgcccagt tgcggctgcg
      181 gctgcgggac gatttgcggc ggatcatcaa gcgggtcggg ctgacggcac tttatgtgac
      241 ccatgaccag caggaagccg tggtactggg cgaccgtatc ggcgtgatgc gcgacggttt
      301 gttgttgcag ctggcggccc ctgacgagtt gtacaaccgt ccagccgacc tgttcgtggc
      361 gggcttcacc ggggcgtcga acgttattgc cggacggctg caatcccgcg acggcgggtt
      421 cggtgccgta tcggttggcg ccggcaattc gctgatcgtt cgcctggcgg gcgatgcggc
      481 accaggttcg gcattgaacg tggccttgcg gcccgacaac gtgttgctgg agccggcaaa
      541 tgacggccca aaccagtttc cgggacgggt ggtatcgcga cattaccagg gcactcagac
      601 ggtctacgaa gtcagtctgt tcggccagac catcgaggcg ctcgaggtcg gcagcagtat
      661 tcgccatgcg gtgaacgacg ctgtaacgat cgcgttgccg gcagaggttt gctggggata
      721 cgcggcagcg gttgaccaca gattacgcgg cagcggttga ccacagacaa tccgtcatgg
      781 ctgtgcttgg cgcggccgcc cacgatttgc ctgaaccagg gaacgtcgtg ggtcgctggc
      841 gcagggccgc ccagacacaa ccagagaagg ccgcgggctg acaacccatc caactcattt
      901 cgagcgaagc acgccatgat gttgctgggc cgtatcaccc ggctcaaaag cggtcggtcg
      961 ccaagaaagc gctagctcag acggccgaca tacgcctcca tcagggccca cgcctcgtcg
     1021 ccaaaagtct tgcgccactg atcatagaac ccgtcccgtc gcaggatcgt ccgaaacggt
     1081 tcgggatcgg gcgtattgaa tgtcattccc tgtgattcca gtttgccctt caaggatgcg
     1141 tcgagcgctg cgatgtccac ccgatcttcc tcggccgcgc cgttcagttc ccgcgatacc
     1201 acgctgcgaa tatcgtccgg caggcctttc caggcccgcc cgttgcacag cagccaatag
     1261 ccatcccaca tatgattggt catcgagacg tatttctgaa cctcggagaa tttcgcggtg
     1321 tcgataatgg acaacgggtt ttcctgccca tccaccacat gagtctgcaa cgccgtatag
     1381 gcttcggcaa agttcaacgt cgccggcgct gcccccagtg ccctgaacat ggagacgcca
     1441 agctggctcg gcgggatgcg tatcttgaag cctttgaagc tgtcaggagc ggtgatcgga
     1501 tgggtactcg aggaaacctc tcggaagccg atatcgaaga tcttgtcgat cgcatgcagc
     1561 ccggctgcgt cgatcctggc tcgaattgcc gtccccagct tgccgtccat cgccggccac
     1621 acctgagtgt aatccctgaa ggcaaaaccg agcgcgttga tggcggtcaa tggaaccagt
     1681 gtcgatagga caaggcctga ggcggtatag aagtcaatcg cgccggaccg gacctgggac
     1741 aacatatcgg tgtcggttcc cagttgtccg gcgggaaaaa tctgcaggtc gaagcggcca
     1801 ttcgtagccg cacggatccg ctccgccgcc tgcgatatcc ggacgttcag gggataagtc
     1861 tccgggacgt tgttgccata tttgaagacg aactccgccg cccgggcgca cacgataccg
     1921 aacgccggtg ctgtcagtgc cgtgccaaca aatgtccttc tgccgaactt cgacatgcct
     1981 tccccccgca aggtgcagcg cctgcgtcgt cttgatcttg cagcttaagg cgcttccgtc
     2041 agcttgcaac agacgtggta tctgctctca gagcggcgtc cagccgagaa aagacccgcc
     2101 gggcgttgcc ctcataaacg gcttccttgg cttcgggcga gagaatcgtc gaagcttcga
     2161 tgtagcgttt cgtgtcgtca tagtagaacc cggtttcagg atcgattccg cgcaccgcgc
     2221 cgatcatctc gctggcaaac aggatgttct tcaccggaat gactttggtc agcaggtcga
     2281 tgcccggctg atgatagaca caggtatcaa aatagatgtt gttcagcagt agttccgtca
     2341 gcaggggttt cttcatctcc tgcgccaggc cacggaaccg cccccagtga tatggcaccg
     2401 ctccgccgcc atgcgggatc aggaacttca gcgtcggaaa atccttgaac agatcggacg
     2461 tcaggcactg catgaacgct gtcgtatcgg cgttcaggta atgcgcgccc gtggtgtgga
     2521 agcagtcgtt gcagcttgtg ctgacatgca ccatcgccgg gacgtcgtat tccaccattt
     2581 tttcgtagat cggataccat tgccggtcag acagcggcgg cgaggtccag tggccgcccg
     2641 atggatcggg attcaggttg atgccgacaa aaccgtactc cttgatgcat ttctccattt
     2701 cagaaataca tgtcctcggg tcgacaccag gcgactgagg cagcatcgcc gcaccgatga
     2761 aatgacccgg aaacaactgg ctgacgcgat agcatagttc gttgcatatg gctgcccagg
     2821 tggcggacac gttgaagtcg ccgatgtggt gcgccatgaa gctggcacgc gggctgaaaa
     2881 tggtcaggtc aatgccacga tcctgcatct ttgcaagctg attgccgatg atgctctcgc
     2941 gcagctcgtc gtcgctgatc ttcaggtcgc tgaccttggg catcgtcgac ttgtcgccaa
     3001 tgccggcaat ctggcggttg cgccacacct ccagcgcctt tggtgcggtc gtataatggc
     3061 catggacatc gatgatcaca aaaatctccc gtcggatgcg cagcacgtgc ccagtgtcac
     3121 ccaccaggac gccgacgac
//
LOCUS       DASWQN010000381         5075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_564438, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000381 DASWQN010000000
VERSION     DASWQN010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564438"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1094..2458)
                     /locus_tag="VGC82_06255"
     CDS             complement(1094..2458)
                     /locus_tag="VGC82_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY0422911.1"
                     /translation="MIKSSDQVDRRVWIIAAACTCGPLMSGLDSTMVNVSLDTIGRAF
                     EAPLGTIQWITSGYLLALALALPLSGWLIDRVGARRIFLGCFCGFVVCSMLCGLAQSV
                     TLLIACRVLQGIAGGLLAPMMQMMMARHAGRQMARVIGVAAMPVMVGQMLGPSLGGLI
                     LTYLDWHWIFFVNVPVGLVAVAFAWRVLPPDEATNARRLDLLGFAMISPGLALLLQGL
                     VSLAHGNPEAPFSLAASAVLLAAFAWHALRRPATALIDLRLFGARTFRAAAATQFLSN
                     AINFGGQLLMPLYFLKLRGLPPSVTGLLLMPMGLGVFFALPIMGRLSERFGARAISGT
                     GAALSLIGTIPFALAGADTPVALLCVALLIRGFGGGSITIPSAAAAYASVPRESLGHA
                     TTAINICQRFGGPVGTSGLAICLQIALTRMQAPEQAYAIAFWVLAGVAAAALLTAAHL
                     PGRD"
     gene            2764..2961
                     /locus_tag="VGC82_06260"
     CDS             2764..2961
                     /locus_tag="VGC82_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422912.1"
                     /translation="MPSTPGAEALDYLLASAGLDLTEAQKADLRTTFDGLAAMKARVR
                     QPRGRMAELAHTYGFTEEDLA"
     gene            2958..4352
                     /locus_tag="VGC82_06265"
     CDS             2958..4352
                     /locus_tag="VGC82_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0422913.1"
                     /translation="MSAFVPGIAEAARLIAAKKLSPVELTQACLGRMEAMDGVLHAFI
                     LPTPERAIADAKAAEARVMKDGPLGPMHGIPIGLKDIVDTAGIATTCQSRILADNVPT
                     TDATCAAQLAASGTVLMGKLTTHEFADGGPSFDLPMPPARNPWNPEHFTAGSSSGTGA
                     AVAAGMILCGIGTDTGGSIRGPAALCGIAGIKPTYGLVSRAGVAPAAYSLDHIGPMAW
                     SAEDCAIMLQVLAGHDPKDPASANRPVPDYTALIGSGLKGVKIGVIRHFHETDHPVER
                     ATQQGIDNALTVFRDLGAEISDVTVSPLQDWHACGSLISITERAAAYEEWSRTRLGDF
                     SERVQRRLMLGALVSGVDYVQAVRRRRELRAELQAAMAGLDVVITAGAPGEAAKMDAI
                     SRWDLFDKPNFTMPFNVSGYPAICVCSGFGPLGLPVSLQIAGKPFREAKVFQVADAFE
                     KATDFRAKRPAMVG"
     gene            4412..>5075
                     /locus_tag="VGC82_06270"
     CDS             4412..>5075
                     /locus_tag="VGC82_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEY0422914.1"
                     /translation="MTTHTLHAAPDTVRIGVFDAEFPAIMTIESGDTVEVQCVSGREE
                     VMPPAGSSMVVPPELAAILAANPGTRAGHIVTGPIAIAGAMPGDMLEIRIEKIVAGAN
                     WGYNMIRPLAGTLPEDFHETTLMHIPVDQERLVCTLPWGTELKMAPFFGVMGVAPPPA
                     FGRIMSKEPRIHGGNLDNKELTAGSTLFLPVWVPGANFSVGDGHGIQGDGEVCVTALE
                     MCL"
BASE COUNT         1001 a         1576 c         1605 g          893 t
ORIGIN      
        1 ccagaaggca tcttcaccgg atcaaatcag agaccttcag cttgcgctac cttccagcct
       61 catcaccccg aaacccaaca agagccaaga tagcagaatc tggaactgat cagtgtatcg
      121 gcgggcttgg tgtccattgc ggtagatact gcgtgcagcg tgtggcgccc attcttctcc
      181 cggcacaccc acagggcgcc cccagacttc gatcgctgac aacgcacgaa gccgcttgcg
      241 tgccatcgtc tctctgagag cctgacccag aatggtaaat ggaacaattt taacccaacg
      301 atcttgtttg cagcggggca gctcgcgcct gacaacaccg gttttctaaa ccattgataa
      361 caccatagat aatcctcgtt ccagccggtg ttgaagacct tcgaaggcga ccaaaccctg
      421 ttcaagccgc gattgccaaa gcttatggcc tttattttca tctgcggctt tttcacgaac
      481 ccgcgacaag ttgaacagtg atccggcccc agcgttactc gacgactaat cgaaactgga
      541 ggaaatcatg agaagctcgt tttgtctcgc cgcgctctgc ttactaagcg cttgtgatca
      601 atacagcacg ggtcagcgcg cggtaggcgg tggtgctctt ggggcgggaa cgggagccgc
      661 tgttggcagt ctagccgggg gccatgccgg cacgggtgcg ttgattggcg gtggcctggg
      721 ggcggccgga ggcgccctga cgactccgtc gcggccctat tccaacggcg gctacgagaa
      781 tggctccagc ggatacagga ctagctcagg tggctacagc agcggcggct atggtagcgg
      841 gggctatgga agcggcggtt acggtagttc gcgctattga gaggctggaa cagcttgcgg
      901 ctgctcacat atcgcataat cgttccactc gatctctagc tggtcgttgc cagtatcggc
      961 ccgcactgag ttagctgccc cagcgctata tcacgtcgcc cttccgtcat tcggccaccg
     1021 actaactccg catacgtgat cgccgatcgg tactgatgtt cgggaatcgg attcgcgctc
     1081 cgggaaagtg cgcctaatct cggccaggca gatgcgccgc cgtcaggagt gccgcggcgg
     1141 ccacgccggc cagaacccag aatgcgatcg cataggcctg ttccggtgcc tgcatccgtg
     1201 tcagggcgat ttgcaggcag attgccaagc cgctcgtgcc aaccggccca ccgaagcgct
     1261 ggcagatatt gattgccgtc gtcgcgtgcc ccagcgattc gcgcggcacc gaagcatagg
     1321 ccgcggcggc cgacggaatg gtgatcgatc cgccgccaaa tccgcgtatc agcaaggcca
     1381 cgcacagcaa agccactggc gtgtcggccc ccgccagcgc gaaagggatg gtgccgatca
     1441 gcgaaagcgc tgctccggtg ccggatatcg cgcgggcgcc gaaacgttcg ctcaaccgcc
     1501 ccatgatcgg cagcgcgaag aacacgccca gccccatcgg catcagtaac agtccggtca
     1561 cgctgggcgg cagaccacga agtttgagga aatacagcgg catcagaagc tgcccgccga
     1621 agttgatggc attcgacagg aactgggttg ccgcggcagc acgaaatgtc cgcgccccga
     1681 acaggcgcag gtcgatcagc gcggtcgccg gtcgccgcag ggcgtgccag gcgaaggccg
     1741 cgagcaggac ggcgcttgcg gccaggctga acggcgcctc cggatttcca tgcgcaagcg
     1801 ataccagccc ttgcagcagc aatgccagac cgggtgagat cattgcaaac cccagcagat
     1861 cgagacgccg cgcgttggtg gcctcgtctg gtggcagcac cctccaggcg aacgcgaccg
     1921 ccaccagccc gaccggaaca ttgacgaaga agatccagtg ccagtcgaga taggtcagga
     1981 tcagtccgcc cagggacggc ccgagcatct ggccgaccat tacgggcatg gcggcaacac
     2041 cgatgacgcg ggccatctgg cggccggcat ggcgggccat catcatctgc atcatcggcg
     2101 ccagcagccc gccggcgatt ccctggagca cgcggcaggc gatcagcaac gtgaccgatt
     2161 gcgcaagccc gcacagcatc gagcagacca caaatccgca aaagcagccg aggaaaatac
     2221 gacgagcgcc cacccgatcg atcagccagc cgctcagcgg caatgccagg gccagtgcca
     2281 gaagatatcc gctggtgatc cattggatcg ttcccagcgg cgcttcgaac gcgcgtccga
     2341 tcgtgtcgag cgagacgttg accatggtgg agtcgaggcc cgacatgagc ggcccgcagg
     2401 tgcaggccgc ggcgatgatc cagacgcggc ggtcaacttg gtcggaggat ttgatcatgc
     2461 gctgggcggc tcacagctgg ggtagagccg attacaggac atctgcccga aagcgccaat
     2521 tgttggctcc gaaccttatc gggtggaaaa gtgctgattc ctgcatccgg gcatccggat
     2581 ttcgggcggg tgcctgcaaa tatcgcagcc acaacgcagc cttccacagg cttttcgata
     2641 tccggctttt cgatagacgg ctcttcggta ttcggcgctc atcgaacacc gcgttcggcc
     2701 cggttcttgc ttgaatacat gatgcgtgtc gtactttcgg ataatgacgc aaggagcctg
     2761 acaatgcctt caacccctgg agccgaggcg ctcgattacc tcctggcaag tgccggactg
     2821 gatctgaccg aagcgcaaaa agccgatctc aggacgacct tcgatgggct tgccgcgatg
     2881 aaggcgcgcg tgcgccagcc gcgcgggcgc atggcggaac tggcgcatac ctacggcttt
     2941 accgaggagg acctcgcatg agtgcgtttg tccccggtat cgccgaagcg gcaaggttga
     3001 tcgcggccaa gaagttgtcg ccggttgaac ttacgcaggc ctgccttggc cgcatggagg
     3061 cgatggacgg cgtgctgcac gcgtttatcc tgccgactcc cgaacgggcg atagccgacg
     3121 ccaaggccgc cgaagcccgg gtgatgaagg acggtccgct cggaccgatg cacggcatcc
     3181 cgatcggtct gaaggacatc gtggacacgg ccggaatcgc cacgacctgc cagtccagaa
     3241 tcctggcgga caatgtgccg acgacggacg ccacatgtgc ggcacaactg gcagcgtccg
     3301 gcaccgtgtt gatgggcaag ctgacaacgc atgagtttgc cgacggcgga ccatccttcg
     3361 acctgccgat gccgcctgcc cgcaacccct ggaacccaga gcatttcacc gccggatcgt
     3421 ccagcggcac cggcgcggcg gtggctgcgg gcatgatcct gtgcggaatc ggcaccgaca
     3481 ccggcggctc gatccgtgga ccggcagcgc tttgcggcat cgccggcatc aagcccacct
     3541 atggtctggt gtcgcgcgcg ggcgttgcgc cggccgccta cagcctggat catatcggcc
     3601 cgatggcctg gtccgccgag gactgtgcga tcatgctcca ggtcctcgcc gggcatgacc
     3661 ccaaggatcc ggcgagcgcg aaccgcccgg ttcccgacta cactgcgctg atcggctccg
     3721 gcctcaaggg cgtgaagatc ggggtcattc gccattttca tgaaaccgat cacccggttg
     3781 agcgggcaac ccagcagggc atcgacaacg cgctgacggt gtttcgcgat ctgggtgccg
     3841 agatcagcga cgtgacggta tcgccgctgc aggattggca cgcctgcgga tcgctaattt
     3901 cgatcaccga acgggcggcg gcgtacgagg aatggtcgcg cacgcggctg ggtgacttca
     3961 gcgaacgggt gcagcggcgg ttgatgctgg gcgcgctggt gtccggcgtg gactacgttc
     4021 aggcagtgcg ccgccggcgg gaactgcggg ccgaactcca ggcggcaatg gctgggctgg
     4081 acgtggtgat caccgccggc gcgccgggcg aggcggcgaa gatggacgcg atctcacgct
     4141 gggacctgtt cgacaaaccg aacttcacca tgccgttcaa cgtctccggc tacccggcaa
     4201 tctgcgtctg ttcggggttc gggccgctgg gactgccggt ttccctccag atcgccggca
     4261 agccgttccg cgaggcaaag gtcttccagg ttgccgacgc cttcgagaag gcgacggatt
     4321 ttcgcgccaa acggccggcc atggtcggat aaaccaaagg cttggttcgg aatgaacgtg
     4381 cgccttctac aatgcgccat agggatatgc catgacgaca catacgctcc acgccgcacc
     4441 ggacaccgtg cgtatcggcg tgttcgatgc cgagtttcca gccatcatga ccatcgaatc
     4501 cggcgacacg gtggaggtgc agtgcgtctc cggccgtgaa gaagtcatgc cgccggcggg
     4561 atcatccatg gtcgtgccgc ccgaactggc cgcgatcctg gcggccaatc caggcactcg
     4621 ggctgggcac atcgtcacgg gtccaatcgc catcgcgggt gcgatgccag gcgatatgct
     4681 ggagatccgg atcgaaaaga tcgttgccgg tgcaaactgg ggttacaaca tgatccgccc
     4741 tcttgcgggc accttgcccg aggacttcca cgaaacgacg ttgatgcata tcccggtgga
     4801 tcaggaaaga ctggtctgca ccctgccgtg gggcaccgaa ctaaagatgg cgccattctt
     4861 tggtgtgatg ggtgtggcgc ccccgcccgc gtttggccgc atcatgtcga aggagccacg
     4921 gatacatggc ggtaacctcg acaataagga attgaccgcg ggcagtacat tgttcctgcc
     4981 ggtctgggtg ccaggtgcca atttctcggt gggagacgga cacggtattc aaggcgatgg
     5041 cgaagtctgc gtcaccgcgc tggaaatgtg cctga
//
LOCUS       DASWQN010000382         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_828906, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000382 DASWQN010000000
VERSION     DASWQN010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828906"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VGC82_06275"
     CDS             <1..887
                     /locus_tag="VGC82_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0422915.1"
                     /translation="TVPDTGWQDDPWSARIDADGMLHGLGSTDMKGPVAAAIIAARQL
                     PVTVPITLLITTDEETTKQGARVMVERSALVRTVRPTGILVVEPTQLQPVRGHRSHIL
                     FTCEAFGIQAHSSKGIGRNANWALIPFLAEMKSLYERLLTDRSLQDDAYDPPFSDFNV
                     VIDNHGTAINVTVPVATARIKFRYSAGIDPAPIRAKVHEAAARYGVRVTEAEEGAPPE
                     LPADHPFVRQCAEASGRSATTAPFGTDASVLQAMAPCVVMGPGDIGVAHKPGEAVRLS
                     DLAAAVPVFQALAMRLVS"
     gene            1033..1974
                     /locus_tag="VGC82_06280"
     CDS             1033..1974
                     /locus_tag="VGC82_06280"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0422916.1"
                     /translation="MDLINGLHVFIRVVETGSFSAVARESNSSQSAITRQVAQLEEHF
                     GVRLLHRTTRKLSLTNEGQDLVIRARHLLDEAEDLEDTFGKNGGSPTGHVRIGLPVGA
                     AMLLVPDFANLLRRFPGLSVEMVVKEQIDDLVAERLDVALRFGQSADTSLMSRAVATL
                     ASTPVAAPSYLERHGAPVHPSDLADHSCIIHDIGPDSNHWVFNGPNGSADVEVTGPFR
                     CDNSLVVRQAAVSGFGIALLGDPLTINDIRTGRLMRLLPDYLARQRQAFIVYPSRRHL
                     AQRTRVVIDYLVEEFRQLELRLRDSREWGENDATWLV"
     gene            complement(1997..>2332)
                     /locus_tag="VGC82_06285"
     CDS             complement(1997..>2332)
                     /locus_tag="VGC82_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422917.1"
                     /translation="IYVDGAGETHWRDVQVEWVEERNGSKLSARLPANGIIFRETAGS
                     YDLDWHPAPRRQYIINLDAGVKITASDGEAREIGAGEVVLVEDVRGKGHLSQSIGGKM
                     RHSIFIPLE"
BASE COUNT          411 a          752 c          765 g          404 t
ORIGIN      
        1 acacggtgcc cgacaccggc tggcaagacg acccgtggtc cgctcggatc gatgccgacg
       61 gcatgctgca cggcttgggc agcaccgaca tgaaggggcc ggtcgcagcg gcgatcatcg
      121 cagcacggca gttgcccgtc acggtcccga tcaccctgtt gatcacgacc gatgaggaaa
      181 ccaccaagca gggcgcccgc gtcatggtcg agcggtcggc gcttgtgcgg acggtgcgcc
      241 cgaccggcat tctggtggtg gagccgacgc agttgcaacc ggtccgcggc caccgcagcc
      301 acatcctgtt cacctgcgag gcgtttggca tccaggcgca cagctccaaa ggcatcgggc
      361 gcaacgcgaa ctgggcgctg atcccgttcc tggcggagat gaaatcactc tacgagcgct
      421 tgctgacgga tcggtcgctg caggacgatg catacgatcc gccgttcagc gacttcaacg
      481 tcgtcatcga caaccacggc accgccatca acgtcaccgt tccggtcgcg acggcgcgga
      541 tcaagttccg ctactccgcc ggcatcgatc cggcgccgat ccgggcaaag gtgcatgaag
      601 cggcggcgag atacggggtg agggtgacgg aggcggaaga gggcgcacct ccggagctac
      661 cggccgacca tccgttcgtc cgtcagtgcg ccgaggccag cggccggtcc gcaacgacgg
      721 ctccgttcgg caccgatgca tcggtgctgc aagcaatggc gccgtgcgtg gtgatggggc
      781 cgggcgatat cggcgtcgcg cataagcccg gcgaagccgt tcgcctgtcg gacctggccg
      841 ccgccgtgcc ggtgttccag gccttggcga tgcggctggt gtcctgagcg gtagcgagcc
      901 gcgatacctg acgatgccgc aaatccgttg acggacccgc ctgatcggga ttgaacgaag
      961 ccgtacggac cgagggggcg ggcgatgccg gccagacgat gtacctgatc aatcgatgga
     1021 cctgatcaat cgatggacct gatcaacggg ctgcacgttt tcatccgcgt cgtcgaaact
     1081 ggctcgtttt ccgccgtcgc gcgggaatcc aacagcagcc agtccgcgat tacccgtcag
     1141 gtcgcccaac tggaggagca tttcggcgtc cggctgctgc atcgtaccac ccgcaagctc
     1201 agtctgacca acgagggcca ggacctggtc atccgcgctc ggcatctgct ggacgaagcc
     1261 gaagacctgg aggacacgtt cggcaagaac ggcggttctc cgaccggcca cgtccgcatt
     1321 ggcctgcctg taggcgcggc catgctgctg gtgccggatt tcgccaacct gctgcgccga
     1381 tttccggggc tgtcggtgga gatggtggtc aaggagcaga tcgacgatct ggtggccgaa
     1441 cggctggatg tggcgctgcg cttcggtcag tcagcggata cgtccctgat gtcgcgggcg
     1501 gtagcgaccc tggcgtccac gccggtggct gccccatcgt acctggagcg tcacggcgcg
     1561 ccagtgcatc cgtcggacct ggcggatcac agctgcatca tccatgacat cggaccggac
     1621 agcaaccatt gggtcttcaa cggcccgaac ggctcggccg acgtggaagt gaccggaccg
     1681 ttccggtgcg acaatagcct ggtcgtgcgg caggcggctg tctcgggctt cggcatcgca
     1741 ttgctgggcg acccgctgac gataaacgac atcaggacgg gacgactgat gcgcctgttg
     1801 ccggattacc tggcgcgcca acgccaggca ttcatcgtct atccgtcacg ccgacatctg
     1861 gcgcagcgta cccgcgttgt aatcgactac ctggtcgaag agttccgcca gttggaattg
     1921 cggctgcggg actcgcgaga gtggggcgag aacgacgcga catggctggt gtagcgtcgc
     1981 aagccccgcg accgctctat tcgagcggga tgaaaatcga atggcgcatc ttgccgccga
     2041 tcgactgcga caaatgaccc ttgccccgga cgtcctcgac caggaccacc tcacctgcgc
     2101 caatctcccg cgcttcgccg tcgctggcgg taatcttcac gccggcatcg aggttgatga
     2161 tgtattgccg ccttggtgcg ggatgccagt cgaggtcata gctgccggcg gtctcacgaa
     2221 aaatgatccc gttggccggc aggcgcgccg acagcttgct gccgttgcgt tcctcgaccc
     2281 attccacctg gacgtcgcgc caatgggttt cgccggcacc atcgacgtag at
//
LOCUS       DASWQN010000383         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1620343, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000383 DASWQN010000000
VERSION     DASWQN010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620343"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..1044
                     /locus_tag="VGC82_06290"
     CDS             382..1044
                     /locus_tag="VGC82_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEY0422918.1"
                     /translation="MFDGDLSTAKGRVVAWIDSLFIDHAVLRLIWGNLATVMPGKIYR
                     CNHPTPARLRRMTERLGLRTLINLRGKTQSGSDALSREAARTLNLDFHDMALESRGAP
                     QRDRIMRLHGIYTSMSTPALMHCKSGADRAGLAAGLMVMFAGGTSAEALKQLSWRFGH
                     IRQARTGILDAFFLRYQREAEGRKPFLDWIRDDYDEAALRRDFHANGLASFINDWVLV
                     HE"
     gene            complement(1172..1393)
                     /locus_tag="VGC82_06295"
     CDS             complement(1172..1393)
                     /locus_tag="VGC82_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422919.1"
                     /translation="MKVLIVGTALAALLAAGPVQAKGCIKGAAVGGLAGHLAHHGVLG
                     AAGGCAVGHHMASQKEKQAEQQQQQTQYR"
BASE COUNT          293 a          502 c          446 g          279 t
ORIGIN      
        1 agatcacttt cgataaggcc ggcatctact actacatctg cgctccgcac ccgtggatgt
       61 acggccagat catcgtagaa taatgtcggc tggcgtgggc ttgacgactc ggggttcctg
      121 gtaggatctg gcactggttc gatgccgctg tcgattggtc gaagcggcat cgcgtcatgt
      181 gcgacctgag ggacagccga tctcgaagag acatgcacca aggcctgcat aaacttagtt
      241 gccgtccgaa ccgcggacgg cgccggcgcg gagttcgtgc cgcaacccgt ccatgaccac
      301 ccaaacagtt gcgctcagac ccccaccatg cccatatccc tggcagtatc caaaggtttg
      361 gccaatccac gcatgaactc catttttgat ggcgacctca gcaccgccaa gggccgcgta
      421 gtcgcctgga tcgactcgct gttcatagac catgcggtgt tgcgcctgat ctggggcaat
      481 ctggccacgg tcatgcccgg caagatctac cgctgcaacc atccaacccc agcccgccta
      541 cggcgcatga ccgagcgcct ggggctgcgt acgctgatca acctgcgcgg caagacccaa
      601 agcggatcgg acgccctttc ccgggaagca gcacgcacgc tcaatctgga cttccacgac
      661 atggcgcttg aaagccgagg cgcgccgcag cgcgaccgga tcatgcgact gcacggcatt
      721 tacacctcta tgtccacacc ggccctcatg cactgcaagt ccggagctga tcgcgcgggc
      781 ctcgccgccg ggctgatggt gatgtttgcc ggcggcacct cggcggaggc gctgaagcag
      841 ttgtcctggc gcttcgggca tatccggcaa gccagaaccg gcatcctgga cgccttcttt
      901 ttgcgctacc aacgcgaagc cgaaggtcgc aagccgttcc tggactggat tcgggacgac
      961 tacgacgaag cagcactgcg gcgggacttt catgctaacg gcctggcaag ctttatcaac
     1021 gactgggtgc ttgttcacga gtaaagcgcc ggggccgtcc cgttgcccgc gccagaggct
     1081 ttagcgcaac ggccgaagga tcccctgcag caacgcccgg aaataccacg cgcccgctgc
     1141 ggacgcgcga acctattggt gacgcgcgaa cctagcggta ctgtgtctgc tgttgctgct
     1201 gttccgcctg cttttccttc tgagacgcca tgtgatggcc gaccgcgcag cctcccgcgg
     1261 ccccgagcac cccatgatga gccaggtgtc cggccaaacc gccaacggcc gcgcctttga
     1321 tgcagccttt ggcctggacc ggacccgccg ccaaaagtgc cgcgagagca gtccccacga
     1381 ttaaaacctt catttcgaac gctcctgcat tgtctctgca gagaaacgct tgaacgcgac
     1441 cgttggttcc ggttagggga acagatcggc gggtgagtcg gctcgaaggc tggcgcccaa
     1501 atccgcaccg atgcgcgccg
//
LOCUS       DASWQN010000384         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2016316, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000384 DASWQN010000000
VERSION     DASWQN010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2016316"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..>1939
                     /locus_tag="VGC82_06300"
     CDS             271..>1939
                     /locus_tag="VGC82_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY0422920.1"
                     /translation="MSRFAGLTPLLAPRSVAVLGASSDATRIGGRPIAYMRAQNFPGA
                     LYPVNPNRTEIQGLKAYPSVADLPETPDVAIVAVPAELAARAIDDLAVRGVKGVIMFT
                     AGFAEMDADGAAAQETMVATARAAGMRILGPNCLGVFDARRSYYATFSSSFDSGWPVL
                     GRIGIASQSGAYGTHLYTLARNRGIGASLCVMTGNEGDVTVGECIGWLAENPDVDVIA
                     VYAEGIREAPGLIAAFEAARAARKPIVMQKVGRSELGTKAAKSHTASIAGDDAVTEAV
                     MAEFGVFRARNSEEMLDIAHTATRKIYPVKNTLGVITVSGGAGVLISDVAESLGLAMP
                     EMPMGAQQKLRALVPFCAPRNPVDATAQVSNDVTLVKTFTESMVRDGGYASVLGFFSM
                     TASSRRWPQIREQLNVVRAENPDRLYVLSVIVPPERREELETDGWVVHEDPTRAVTAI
                     DAMGRYGVAFAAKAADPAPAVPSVTLPAAAPSEVEAKRLLAGAGIASAPEAACATPEE
                     AVAAARRFGFPVVMKILSPDIVHKSEIGGVLLDVIDANAVREGFSLLL"
BASE COUNT          333 a          576 c          690 g          340 t
ORIGIN      
        1 tgacgatcgc cggaatacgg gagcctgctt cgttggacct gctgggtctc caacggggcg
       61 gatagaaacc ggctccgcac cacgtgcggc atctcgaggg gaacttcagc cgtgttggcg
      121 ttgccgaaac acccggcgga aggtcgtcga taagcgggcg cggagcgctg tagcggtcag
      181 ccgcgatggt tgcgatacga tggcacccgg tccaggataa cacgcggcgg gcgaaccgcc
      241 ctgtcataat cttggggaat gtccgtcatc atgtctcgtt tcgccggctt gacgccgctt
      301 ctggcacccc gctccgtcgc tgtcctgggt gcgtcttccg acgcgacacg catcggcgga
      361 cggccgatcg cgtatatgcg ggcgcaaaat ttccccggtg cgctgtatcc ggtcaatcca
      421 aatcggacgg agatccaggg gctgaaagcc tatccgtcgg tggcggacct gcctgaaacg
      481 ccggacgtgg cgatcgtcgc tgttccggcc gaactggcgg cacgagcgat cgacgacctg
      541 gccgtgcgtg gcgtcaaggg cgtgatcatg ttcaccgccg gcttcgcgga aatggacgcg
      601 gacggcgcgg cggcgcagga gacgatggta gcgaccgcgc gtgccgccgg catgcgcatc
      661 ctggggccaa actgcctggg ggtattcgac gctcgtcgat cctattacgc aacgttctcg
      721 tcgtcgttcg acagcggctg gccggtgctg ggccgcatcg gcatcgccag ccagtccggc
      781 gcctatggca cgcatcttta taccctggcg cgcaaccgcg gcattggcgc ctcgctgtgc
      841 gtcatgaccg gcaacgaagg cgatgtgacg gtcggcgaat gcatcggctg gctggcagaa
      901 aatcccgatg tggacgtgat cgcggtttat gccgagggca tccgggaagc gcccggtctg
      961 atcgccgcgt tcgaagcggc ccgggcggcg agaaagccga tcgtgatgca gaaagtcgga
     1021 cgcagtgaac tcggcaccaa ggcggcgaag tcgcacactg cgtcgatcgc cggcgacgac
     1081 gccgtcaccg aggcggtaat ggcggaattt ggcgtgttcc gggcgcgcaa cagcgaggag
     1141 atgctggata tcgcccatac cgcgacgcgg aagatctatc cggtcaagaa cactcttgga
     1201 gtgatcacgg tatccggtgg ggcgggcgtg ctgatcagcg acgtggcgga aagcctcggg
     1261 ctggcgatgc cggaaatgcc gatgggggca cagcagaagt tgcgagcact ggtgccgttc
     1321 tgtgcgccgc ggaacccggt ggatgcgacc gcccaggtgt cgaacgatgt aacgctggtc
     1381 aagacgttca ccgaatccat ggtgcgcgat ggcggctatg cctcggtgct cgggttcttc
     1441 agcatgacgg cgtcatcgcg ccgctggccg cagattcgtg agcagttgaa cgtggtgcgc
     1501 gccgaaaatc cggaccggct ttatgtgctg tcggtcatcg ttccgccgga gcgacgcgag
     1561 gaactggaaa ccgatggctg ggtcgtgcac gaggatccga cgcgcgcggt gacggcgatc
     1621 gatgcgatgg gccgttatgg cgtggcgttc gctgccaaag cagccgatcc ggcgccggcg
     1681 gtcccatccg tgacgctacc ggcggctgcg ccatccgagg tcgaggcgaa acggctgctg
     1741 gccggtgcgg ggatagcgtc cgcacccgaa gccgcctgcg cgacgcccga ggaggcggtc
     1801 gcggcggccc ggcggttcgg cttcccggtg gtgatgaaga tattgtcgcc ggacatcgtg
     1861 cacaaatccg aaatcggcgg cgtgctgctg gatgtgatcg atgcgaacgc ggtacgggaa
     1921 ggtttttccc tgttactcg
//
LOCUS       DASWQN010000385         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1488172, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000385 DASWQN010000000
VERSION     DASWQN010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488172"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            452..823
                     /locus_tag="VGC82_06305"
     CDS             452..823
                     /locus_tag="VGC82_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422921.1"
                     /translation="MGFSSIAFLFFFLPIFLAAYFAMPTIRAKNLVCLTASLLFYASR
                     DAQSLPILLVAIAINTYAALAIDRRQGRARGGALAATIVIDLLILVVFKYAGFLGGIL
                     AQAVHPFGLRLPTPNTDRRLR"
     gene            896..1210
                     /locus_tag="VGC82_06310"
     CDS             896..1210
                     /locus_tag="VGC82_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0422922.1"
                     /translation="MTVQITRRDHDADALREHASRVSEAGVVRRLLALALVLEGHSRA
                     QAAKTCGMDRQTLRDCVHRYNEQGICGLSDRPHGGGAPPKLAARGEDATRGLGQPRAR
                     YC"
BASE COUNT          284 a          494 c          511 g          336 t
ORIGIN      
        1 tatgagcctg accggaaatg acttcggaac gaccacaaag gcggcctgtt ctgctacttc
       61 cgccgatcgg gccggcacca agcgaccagt tcaagctggc gccgacctct atgcatgatc
      121 aggtcggcgt ggctcccgca aaaccagctg aggaaagtgc gtaagcagga taggatttgg
      181 gacattcagc cggaaagacc aggttgacga aataagacgg gtacccgcct catcgcgtgg
      241 ctggttgcgg tcagtagtaa gactgtgctg gggggggcca ccccaaacct gcgcccaaca
      301 tcatcgcaag gcccgaggcg cggtggcatc gcccgacccg tcacatcatc aacggatcat
      361 gactaatcaa catcctctcg ggcacgaact tggatgccgg gtcaggatgt cgtggcggtc
      421 ctccgccttt agtgctgact gacgttctgc catgggcttt tcatctatcg cgtttctgtt
      481 tttctttctg ccgatcttcc tggctgcgta ctttgccatg ccgacgatac gagcgaagaa
      541 tctggtttgc ctgacggcgt cgctgctttt ctacgcctcg cgcgatgcac aatcgctgcc
      601 gatcctgctt gttgccattg ccatcaatac ctatgccgct ctcgccatcg acaggcgtca
      661 gggccgtgcg cggggcggtg cgctggccgc aacgatcgtc atcgacctcc tgattctcgt
      721 ggtcttcaag tatgccggct tcctgggcgg cattctggcg caggcagtgc atccgttcgg
      781 cctccgcctg ccaacgccta ataccgaccg acggttgcgt tgactcgtac gggatttccg
      841 ccggctcgat ggggcgtcat tcgatctgtc cgttctggat acgaggcgag cggccatgac
      901 ggtgcagatt acacggcgag atcacgatgc ggatgcatta cgcgaacatg cgtcgcgggt
      961 ttccgaggct ggtgtcgtgc ggcggcttct ggccttggcg ctggtgttgg agggtcatag
     1021 tcgtgcgcag gccgccaaga cctgcgggat ggatcggcaa accctgcgcg actgtgtaca
     1081 ccgctacaac gagcagggga tctgcggcct gtcggatcgc ccgcacggcg gtggtgcacc
     1141 accgaagctg gcggcccgag gagaagacgc aactcggggc ctgggtcagc caagggccag
     1201 atattgctga aggcggtgtg gttcgctggc gattgcgcta tctgcgggac cggatcctgg
     1261 cgcgtttctc tgtgccgatg gacgaacgca gcgttgggcg gatcctgaag acgttgacgt
     1321 tcagccacat ctcggtgcgc gcccgccatc ctcaagccga tcgagttgtg gtggcaggac
     1381 gaagcgcgcg ttggtcagca aggcagcctg accggcatct gggccaaccg cggcagccga
     1441 ccaattgctc cgcgccgctc gacatcatcg gctggtttgt ccagcgctgg ccgctcgagg
     1501 tcatctttcg cggtgcgcga tcaccgggga gtggaaaccc agtggcaatg gacggacaaa
     1561 gccatcgcgc acacaacctc cctgtctgtt ggggctgttc tccctcgtcg ccttgctcag
     1621 cgccc
//
LOCUS       DASWQN010000386         7196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2544431, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000386 DASWQN010000000
VERSION     DASWQN010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7196
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2544431"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VGC82_06315"
     CDS             <1..250
                     /locus_tag="VGC82_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422923.1"
                     /translation="AAISLAAYVLIAALAPRPTVAILSAGVAAILGLTGHDLRRWALE
                     HRGYLLVHVLMANNIDEAFVRLLTSRPELASRYRPEPA"
     gene            250..885
                     /gene="hisH"
                     /locus_tag="VGC82_06320"
     CDS             250..885
                     /gene="hisH"
                     /locus_tag="VGC82_06320"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY0422924.1"
                     /translation="MRIAVVDYGSGNLASASRALALAAERAGIVATVAVTADPATVAA
                     ADRIVLPGQGAFADCAAGLSAVPGMRNAIEAATDRGAPFLGICVGMQLMAERGLEHSV
                     TPGFGWIKGDVAEMPAPGLRLPQMGWNGLDFVARSHPLLDGLTSGDHVYFVHSYALVH
                     GAPGETIATTDYGGTVPAIVVAGNRAGTQFHVEKSQDVGIRLLTNFLRWTP"
     gene            888..2213
                     /gene="hisA"
                     /locus_tag="VGC82_06325"
                     /pseudo
     CDS             888..2213
                     /gene="hisA"
                     /locus_tag="VGC82_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630316.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
     gene            2307..3077
                     /gene="hisF"
                     /locus_tag="VGC82_06330"
     CDS             2307..3077
                     /gene="hisF"
                     /locus_tag="VGC82_06330"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY0422925.1"
                     /translation="MLKLRVIPCLDVKDGRVVKGVNFVSLRDAGDPVEQAAVYDAAGA
                     DELTFLDITASHENRDTILDVVSRTAARVFLPLTVGGGIRSVDDMRRLLLAGTDKCSI
                     NSAAVARPELVREAATKFGSQCVVVAVDAKRAASGTWSVFTHGGRNDTGIDAIDWCRQ
                     VVSLGAGEILLTSMDRDGTGTGFDLDLLRAACDAVRVPVVASGGVGTLQHFVEGARAG
                     ATGLLAASVFHFGTFTIAQVKQALADAGLPVRLPRSVA"
     gene            3077..3589
                     /locus_tag="VGC82_06335"
     CDS             3077..3589
                     /locus_tag="VGC82_06335"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEY0422926.1"
                     /translation="MAKSAKKRTAARPGASRAKSSQKSPGKASRKPSGKSKKSRAAND
                     VAAVSGSDVAVLDRLFAVVDQRRDADPAISHSARLLSRGPAKVAQKFGEEAVECLIEA
                     VAGNRDALVAESADVLYHLLVLWVSSGVSPSDVWAELVRREGISGIAEKAARVQTVSV
                     RARLGTSKIP"
     gene            3681..4058
                     /locus_tag="VGC82_06340"
     CDS             3681..4058
                     /locus_tag="VGC82_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEY0422927.1"
                     /translation="MPVSGLPPYDDQNIFARILRGEIPCQKAYEDEFALAFHDINPQT
                     PTHILVVPKGPYCSFSDFSAKASDAEIAGFIRAVGKIAGDLGLEPSGYRLVTNMGAIA
                     GQEVAHFHVHLFAGRPLGRMIPR"
     gene            4138..5448
                     /locus_tag="VGC82_06345"
     CDS             4138..5448
                     /locus_tag="VGC82_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0422928.1"
                     /translation="MSATSDARPKRSAVATVLRVTSGNFLEMFDFFLFGFYATYISKA
                     FFPAGSEFASLMLTFMTFGAGFLMRPLGAVILGAYVDQIGRRKGLILTLSIMASGTIL
                     IAFVPDYGTIGLLGPVLVLIGRLLQGFSAGVELGGVSVYLSEMATAGHKGFYVSWQSA
                     SQQVAIMVAAVLGYALSNMLTPPQIGSWGWRIPFVIGCLIVPFLFYIRRSLEETEEFL
                     ARKHRPSTQEIFASIGKNWAIVLLGMMLVVMTTVSFYAITVYTPTFGKSVLKLTTTDS
                     LIVTFCVGLSNFIWLPVMGALSDKVGRKPLLLLFSALTLLTAYPALAWLVAAPSFVNM
                     LLVLLWLSFLYGSYNGAMVVALTEIVPVEVRTAGFSLAYALATALFGGFTPLVSTWLI
                     ETTGNQAAPGMWMAFAGACGLIATILIYSRGAVGARVARAGEPA"
     gene            complement(5595..6884)
                     /gene="coaBC"
                     /locus_tag="VGC82_06350"
     CDS             complement(5595..6884)
                     /gene="coaBC"
                     /locus_tag="VGC82_06350"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY0422929.1"
                     /translation="MTTEPLGEPSGKVPGVAPASHLSSGELHGKRVLLIVSGGIAAYK
                     ALELIRLLRRAGCGVTCLLTDNGGQFVTKLSLQALSESKVYSDLFSLTDESEMGHIQL
                     SRAADLLVVAPASANILAKMAAGSADDLASTVLLATDKPVLVAPAMNVRMWLHPATQA
                     NMATLVRRGVRVVEPNEGAMACNEFGPGRLAEPPEILAAIERLLAPSPCPSGGLLGGR
                     HALVTSGPTHEPIDPVRYIANRSSGRQGHAIAAALAELGARVTLVAGPVTVPDPPGVT
                     VRHVESAREMLDACQSALPADIAVFAAAVADWRVEHPSSGKIKKQPGSAPPSLTLIPN
                     PDILATIAAPGPDRPRLVVGFAAETDDLKANARRKLARKNADWIVANDVSPQTGIMGG
                     AENAVHLVSAAGIETWPRMAKDDVARRLARRIAETLA"
     gene            complement(6881..>7196)
                     /locus_tag="VGC82_06355"
     CDS             complement(6881..>7196)
                     /locus_tag="VGC82_06355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0422930.1"
                     /translation="NIPSALQEANRVLKRGGRFFCMEFSSSDWPGFGKLYEAWASNVI
                     PRIGKAVADDEDSYRYLAESIRTFPNQEKLAAMMRQAGLARVAVRNLSGGIAAIHSGW
                     RL"
BASE COUNT         1149 a         2309 c         2373 g         1365 t
ORIGIN      
        1 cgccgcgatc agtctggctg cctatgtcct gatcgcggcg cttgccccgc gcccgacggt
       61 cgcaatccta tcggcaggcg tagcggcaat cctggggctg accggccacg acctccggcg
      121 ctgggcgctc gagcaccgcg gctacctgct ggtccacgtt ctgatggcca acaacatcga
      181 tgaggccttc gtccggttac tgaccagtcg ccctgagctg gccagccgct accggccgga
      241 gccggcttga tgcggatcgc ggtcgttgac tacggcagcg gcaacctggc atccgcttcg
      301 cgcgccttgg cgctggcagc cgaacgcgct ggaatcgtgg ccaccgttgc cgttaccgcc
      361 gaccccgcga cagtggccgc cgccgatcgc atcgtcctgc ccggccaagg cgccttcgcc
      421 gattgtgccg ccggcttgtc ggcggtcccc ggtatgcgca atgcgatcga agccgccact
      481 gaccgaggcg cacctttcct cggtatctgc gtcggcatgc aactgatggc cgaacgtggc
      541 cttgagcata gcgtcacccc cggttttggc tggatcaagg gcgatgtggc ggagatgccg
      601 gcgcccggcc tgcggctgcc gcaaatgggc tggaacggcc tggacttcgt ggcccgctcg
      661 caccccctgc tggacggcct gacgtccggc gatcacgtct atttcgtcca tagctacgct
      721 ctggttcacg gcgcaccggg cgaaaccatc gccaccaccg attacggcgg caccgtgccg
      781 gcaatcgtgg ttgccggaaa ccgcgccggt acccaattcc atgtcgagaa aagccaggat
      841 gtcggcatcc gcctgctgac caacttcctg cgttggaccc cctgatcatg tcggacatca
      901 cccttagccc ggaacccgaa ctccttatcg agcggttgaa gcagttcaac gatcatgatc
      961 tgagcacgct ctgtgaggcg accgacgccg ccatcatcga cggtgggggc ttcggctggg
     1021 tcacgccgcc gggacggcgt gcgctggaaa cctattttcg cggcgtcttg ctggtcccgg
     1081 agcgcgagtt gttcgtcgcc cgcctgaacg gcgacatcgt cgggtctgcc atcctggtgc
     1141 gtccgccgcg caacaacgag gctcaggcct tcgctgccaa cctgatgcac tcctacatcg
     1201 ccccctatgc gcgcggtcac ggattggccc gcatgttgac ccgtgcggtg gaggagcggg
     1261 ctcgggacct cggttaccac atcctgaacc tggacgtgcg ggaaacccag gcggtggcaa
     1321 tcgcgatgta cacctcgcac gggtatatac gttggggcga gcatcccgag tatgcccagg
     1381 tgcgcggcaa gtcgattgcc ggcttcttct tttacaaacg gctccggccc gcgaaatgat
     1441 cggtgcctta tgatcgtccc gctccgatga ccgggttgac gctgtacccg gccatcgacc
     1501 tgaaagacgg caaatgcgtc cgattgcgcc ggggcgatat ggatcaggcg acgatttatg
     1561 ccgacgatcc gggcgcccag gcgaaagcgt ggcaggacgc ggggtttgcg tggctgcatg
     1621 tggtggacct ggatggcgcc ttcgccggtc ggcccgtcaa tgccgccgct gtccggtcga
     1681 tcctggctaa cgtttccatc ccggtgcaac tgggcggcgg aattcgcgac ttggccggcg
     1741 tcgccgcctg gctcgacgcc ggcgtccgtc gggtcatcct gggcagcgcc gccgccaaga
     1801 agccttcgct ggtgatcgag gcgtgcaagg ctttccccgg ctctgtcgcc gtcggtatcg
     1861 acgcccgcga cggtcttgtc gccaccgaag ggtgggccga aacgtcctcg atgcccgctg
     1921 ccgaactagg gctgcgtttc gaggacgccg gggtgtcggc gatcatcttc accgatatcg
     1981 gccgcgatgg catgctgggc ggactgaata tcgatcagac cgtggccctg tcgaaccggc
     2041 tgtccacgcc ggtgatcgct tccggtggtg tcggctccct tgaccatttg gcggcgctga
     2101 aaaccgcggc ggagtcgacc cggatcgagg gtgtcatcgt tgggcgagcg ctttatgacg
     2161 gccgagtcga tcctgccgaa gcattgtcgc ttctggcggc gtggtccgga taaatcccta
     2221 tgtcatccgg tatcctggag gtccgcgcag atattacccg cgtatgtcct tgcatcctgg
     2281 tcaagggcgc tttctaaagc ctcgttatgc tgaaactccg cgttattccc tgcctggacg
     2341 tgaaggacgg ccgcgtcgtg aagggcgtga acttcgtgtc cctgcgcgat gccggcgatc
     2401 cggtggagca ggctgccgtt tatgatgccg ccggcgcgga cgaactgaca ttcctcgaca
     2461 tcaccgcgag ccatgagaac cgggacacca tccttgatgt cgtgtcgcgc accgccgcgc
     2521 gcgttttcct gccgctgacc gtcggcggcg gcattcgttc agtggacgat atgcgccgcc
     2581 tgctgctcgc cgggaccgac aaatgcagca tcaactccgc ggcggtcgct cgcccggagc
     2641 tggtgcggga agccgcgacg aagttcggca gccagtgtgt ggtcgttgcg gtcgatgcca
     2701 aacgggccgc gtccgggaca tggtcggttt tcacccatgg tggccgcaac gacaccggca
     2761 tcgatgcgat cgactggtgc cgccaggtgg tgtcattggg cgccggcgaa atcctgctga
     2821 ccagcatgga tcgcgacggc accggaaccg gcttcgacct cgatctcctg cgcgcggctt
     2881 gcgatgccgt gcgagtgcct gtggtggcct ccggcggtgt cgggacgctg caacattttg
     2941 tcgagggcgc cagggcaggg gccaccggcc tgctcgccgc cagcgtattc catttcggaa
     3001 cgttcacaat cgcgcaggtc aagcaagcgc tggctgacgc cggtctgccg gttcgcctgc
     3061 ctcggtcggt ggcctgatgg ccaaatcggc aaagaaaagg accgccgccc gcccgggggc
     3121 gtcgcgcgcg aagtcatcgc aaaagtcgcc aggaaaggcc tcgcggaagc cgtccggaaa
     3181 gtcgaagaag tcgagggccg ccaacgatgt tgctgccgtc tcgggatcgg atgtggcggt
     3241 tctggaccgc ttgttcgcgg tcgtcgacca gcgccgtgac gccgatcccg cgatcagtca
     3301 ctccgctcgg ttgctctcgc gtggccccgc aaaggtcgcg cagaaattcg gcgaggaggc
     3361 cgtcgagtgc ttgatcgagg ctgttgcggg gaatcgcgac gcgctggtcg ccgagagtgc
     3421 cgacgtgctt taccatctgc tggtattatg ggtgtcctcc ggcgtcagcc cgtccgatgt
     3481 atgggcggaa ctggttcgcc gcgaagggat cagcggaatt gctgagaaag cggctcgcgt
     3541 ccagaccgta tcggttcgcg ccagactggg aacctcgaag atcccgtagg ttcagccggc
     3601 tgtgcaagtg ctctcggccc ggggtcgcaa gctgccagtc ggcctcgtag tgtggcaggg
     3661 acaatgccca ggagacgtcc atgccagtca gcggcctgcc accctatgac gaccagaata
     3721 tcttcgcgcg catcctgcga ggagaaatcc cgtgccagaa agcctatgaa gacgagttcg
     3781 cgctcgcgtt ccacgacatc aatccgcaga cgccgacaca tatcctggtc gtcccgaagg
     3841 ggccgtactg ttccttttcc gacttcagtg ccaaggcgag cgacgctgaa atcgccggct
     3901 tcatccgcgc cgtcggcaaa atcgccggcg atttggggct ggaaccgtcc ggctatcgcc
     3961 tggtcacgaa catgggcgcc atcgcaggac aggaagtggc acactttcac gtccacttgt
     4021 tcgccggccg tccgctcgga cggatgatcc cgcgttgact ctgctatggc gggagacgcc
     4081 ttccgcttgc tctgcattgg gatccagtcg agcgatcggg agaaggtagg aaaatcgatg
     4141 tcggcaacat ccgatgcgcg tcccaagcgt tccgccgtcg ccaccgtcct gcgagtgacg
     4201 agcggcaact tcctggagat gttcgacttc ttcctgtttg gcttctacgc cacgtacatc
     4261 tcgaaggcgt ttttcccggc gggcagcgag tttgccagcc tgatgctgac cttcatgacc
     4321 ttcggcgccg gcttcctgat gcggcccctc ggtgctgtca tcctgggtgc ctacgtcgat
     4381 cagatcggcc gccggaaagg cttgatcctg accctgagca tcatggcaag cggcaccatc
     4441 ctgatcgcct tcgtccccga ttatggcacc atcggtttgc tcggcccggt tctggtcctg
     4501 atcggacggc tgctgcaagg cttctcggct ggcgtcgaac tcggcggcgt gtcggtgtat
     4561 ctgtcggaga tggcgaccgc cggccataaa gggttttatg tctcctggca gtccgccagc
     4621 cagcaggtgg cgatcatggt cgccgccgtg ctgggctatg ccttgagcaa catgctgacg
     4681 ccgccgcaga tcggctcctg gggctggcgc atcccgttcg tcatcggctg cctcatcgtg
     4741 ccgttcctgt tctacattcg ccgctcgttg gaggagaccg aagaattcct ggcacgcaag
     4801 caccgtcctt cgacgcagga gatatttgcg tcgatcggca agaactgggc gatcgtgctg
     4861 ctgggcatga tgctggtggt gatgacgacc gtctcgttct acgcgatcac ggtctatacg
     4921 ccgaccttcg gcaagagcgt gctgaagctg accacgaccg acagcctgat cgtgacgttc
     4981 tgcgtgggcc tgtcaaactt catttggctg ccggtgatgg gcgcgctgtc ggacaaggtg
     5041 ggtcgcaagc cgctgttgct gctgttctcg gcactgacct tgctgacggc atatccggcg
     5101 ttggcatggc tggtcgccgc accaagcttc gtcaacatgt tgttggtgct gctttggctg
     5161 tcgttccttt atggcagcta caacggggcg atggttgtgg ccctgaccga gatcgtacca
     5221 gtagaggtcc gtaccgctgg attctcgttg gcctatgcgc tcgccaccgc gctgttcggc
     5281 gggttcaccc cgctggtctc cacctggctg atcgagacga ccggcaacca ggcggcgccc
     5341 gggatgtgga tggcgttcgc cggggcctgc gggctgatcg ccacgatcct gatttacagc
     5401 cgcggcgcgg ttggcgcgcg cgtggcgcgg gcgggcgagc cggcgtaaca ctgtcgcaag
     5461 gtggccgcgg ttgtggatgt cagtgcgccg tcatcgggtc cggcccacct ggttgaccgc
     5521 accggttcag cggtcgctgg ttggctatgt ggatgaccgt tcaggctgga tgaccgttca
     5581 gggcggatga ccgttcaggc cagcgtctcg gcgatgcggc gggccagccg gcgggcgacg
     5641 tcgtccttcg ccatgcgcgg ccaggtctcg atcccggcag ccgacacgag gtgcaccgcg
     5701 ttttcggccc cgcccatgat gcccgtctgc ggcgacacgt cgttggcgac gatccagtcc
     5761 gcgttcttgc gtgccagctt gcgccgggca ttcgccttca gatcgtcggt ctcggccgcg
     5821 aagccgacca cgaggcgcgg ccggtctggc ccaggggcgg cgatggtcgc caggatatcc
     5881 ggattcggta tcagggtcag gctgggaggc gccgagcctg gctgcttctt gattttgccg
     5941 ctggaggggt gctccacccg ccagtcggcg acggcggcgg cgaaaaccgc gatgtcagcg
     6001 ggcagggccg actggcaggc gtcgagcatt tcgcgggcac tttcgacatg gcgaaccgtc
     6061 acgcccggtg gatcgggaac ggtcaccggc ccggcgacca gggtaacccg cgcacccagc
     6121 tcagcgagcg ccgcggcgat agcgtggcct tgccgaccgg aggagcgatt ggcgatatag
     6181 cgcaccgggt cgatcggctc gtgcgtcggg ccactggtga ccagggcatg acggccgccg
     6241 agcaggccac cggacggaca aggcgacggc gccagcagac gctcgatcgc ggccaggatc
     6301 tccgggggtt cggcaagccg gccggggccg aactcgttgc aggccatggc gccctcgttc
     6361 ggctccacga cccgcacccc acggcgcacg agcgtcgcca tgttggcttg cgtcgccgga
     6421 tgcagccaca tgcggacgtt catggccggc gccaccagca ccggtttgtc cgttgccagc
     6481 agcacagtgg aagcaaggtc gtcggccgac ccggcagcca tcttggccag aatgttggca
     6541 gacgccggcg ccaccaccag cagatcggcg gcccgcgaga gctggatgtg gcccatctcg
     6601 gactcgtccg tgagcgagaa cagatccgag tacaccttcg actcgctcag cgcctgcagc
     6661 gaaagcttgg tcacgaattg tccgccgtta tccgtcagca ggcaggtgac gccgcagccg
     6721 gcgcggcgca gcaagcggat cagttccaat gccttataag cggcgatgcc tccggaaacg
     6781 atcagcagga cgcgcttgcc gtgcagttca ccggatgaga gatgagatgc gggcgcaacg
     6841 ccgggaactt tacccgaagg ctcgcccaag ggctcagtcg tcatagccgc cagcccgaat
     6901 ggatcgccgc gatcccgccg gataggttgc ggacagcgac cctggccaac ccggcctggc
     6961 gcatcatcgc agccagcttt tcctgattgg ggaacgtgcg gatactttcg gcgaggtagc
     7021 ggtagctgtc ctcatcgtcg gccactgcct tgccgatgcg cggaatgacg ttcgacgccc
     7081 aggcttcata gagcttgccg aagccaggcc agtcgctcga agagaattcc atgcaaaaga
     7141 agcggccgcc gcgtttcagc acgcgattcg cctcttgcag ggcggagggg atgttg
//
LOCUS       DASWQN010000387         1886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1884873, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000387 DASWQN010000000
VERSION     DASWQN010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884873"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VGC82_06360"
     CDS             <1..1083
                     /locus_tag="VGC82_06360"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEY0422931.1"
                     /translation="TSCAICGSDLHLFHNFIPAMLPGDIMGHEMMGEVVEVGSGVDSG
                     RLRKGDRIVVPFTITCGECDQCKRGNFSVCEVSNRRKDLANKVFGHTTAGLFGYTHLT
                     GGYPGGQAEYLRVPFADSTNIKVPDSLSDEQVLFLGDILPTGWQAAVHCDIEPTDTVA
                     VWGCGPVGQMAIRSAILLGAKRVIAIDRLPERLSMAEAAGASTINFADEYVVEQLNEM
                     TSGKGPEKCIDGVGLENHVSLTMPDTVYDRAKQMMLLENDRGHVLRQMIYVCRPAGII
                     SIPGVYSGLVDKIPMGQAMNKGLTFRMGQTHVNRWTDDLLRRIEEGQIDPSFVITHTV
                     RLQDGPEMYKVFRDKQDSCIKVVLKP"
     gene            1080..1616
                     /locus_tag="VGC82_06365"
     CDS             1080..1616
                     /locus_tag="VGC82_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422932.1"
                     /translation="MNYISNIVRSEGDPKIISTGPSSANGWDSLGRALGWFSLGLGVL
                     EVLAPRRITRALGVEGNEALVRAYGAREIGSAMLTLSTEKTTGLWARVAGDGLDVVSL
                     AAAYTSRNPKRENVGLAIAAVVGVAALDLIGLWSTISRHGHPTSGWRSYNDRSGFPQG
                     IEKARGAAMAKVAEKATA"
BASE COUNT          375 a          536 c          610 g          365 t
ORIGIN      
        1 acaagctgcg ccatctgcgg ctccgacctg catctttttc acaacttcat tcccgcgatg
       61 ctgcccggcg acatcatggg gcatgaaatg atgggcgagg ttgttgaagt cggctcaggc
      121 gtcgatagtg gccgccttcg gaagggcgac agaattgttg tgccgttcac catcacctgc
      181 ggtgagtgcg accagtgcaa gcgcggcaat ttctcggtct gcgaggttag caaccggagg
      241 aaggacctgg ccaacaaagt gttcggccac acgaccgccg ggttgttcgg ctatactcac
      301 ctgacgggtg gttatcctgg cggacaagcc gaatatctgc gggttccatt cgccgattcg
      361 acgaacatca aggttcccga cagcctttcc gatgagcagg tgcttttcct cggggacatt
      421 cttccgactg gctggcaggc cgcggtccac tgcgacatcg agccaacgga taccgtcgcc
      481 gtctggggtt gcggtccggt cggccagatg gcgatccgca gcgccatact gcttggtgcg
      541 aaacgagtca tcgcgataga ccgtttgccg gagcgcctga gcatggcgga ggcagcgggc
      601 gccagcacga tcaatttcgc cgacgagtat gtcgtcgaac aattgaacga gatgacgtcg
      661 ggcaaaggtc ccgagaaatg catcgacggg gtcgggctgg aaaaccatgt ttccctgacg
      721 atgccggata cggtttacga ccgcgcgaag cagatgatgc tgctggaaaa cgaccggggg
      781 catgtcttgc gtcagatgat ctacgtttgc cggcctgccg gcatcatctc gattccgggc
      841 gtttacagcg ggctggtgga caaaattccc atgggccagg cgatgaacaa gggcttgacg
      901 ttccggatgg gccagacgca cgtcaatcgc tggaccgacg acctgctgcg ccgcatcgaa
      961 gagggccaga tcgatccgtc attcgtcatc acccataccg tcaggctgca agatgggccg
     1021 gagatgtaca aggtattccg cgacaagcaa gatagttgca tcaaggtggt actgaagcca
     1081 tgaactatat ctcgaacatc gttcgctccg agggcgatcc caagatcatt tccacgggtc
     1141 cgagttctgc caacggatgg gacagtctgg gccgagcgct cggctggttc agcctgggcc
     1201 ttggcgtcct tgaggtgttg gcgccgcggc gcatcacgcg ggcgcttggc gtggaaggca
     1261 acgaagcgct ggtacgcgcc tatggcgccc gcgagatcgg cagcgcgatg ctgacgcttt
     1321 ccaccgaaaa gaccactggg ctatgggcca gggttgcagg cgatgggctc gatgtcgtgt
     1381 ctctggcggc ggcgtacacc tcacgcaatc cgaaacgaga gaatgtgggc ctggccatcg
     1441 ccgcggttgt cggcgtggcg gcactcgatc tcatcggtct gtggtccacg atcagccggc
     1501 acggccaccc caccagcggc tggcggtcgt ataacgaccg gagcggcttt ccacagggca
     1561 tcgaaaaggc gcgcggcgcc gcgatggcca aagttgcaga gaaagcgacc gcctgaggcg
     1621 aaaatgactt cgatccagca ctggccgatc cagcgctggc cgatccagca ctggccgatc
     1681 ctgcactgac aaggaaagga ggcgcttcag gccggtgttg gtagttgatt ttggcccgat
     1741 gcctcaggca gcatcgttac gggcgctggt cggcgtttga atcgctcggc aacattcccg
     1801 gtggatcgat cgtgtcgaag tcgaagctga agatgaagtc agtctccgga ccgcggcggc
     1861 aatatgcggc gctttcggtg cagttg
//
LOCUS       DASWQN010000388         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1225258, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000388 DASWQN010000000
VERSION     DASWQN010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225258"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1199
                     /locus_tag="VGC82_06370"
     CDS             105..1199
                     /locus_tag="VGC82_06370"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY0422933.1"
                     /translation="MHRRTLLSTLPVATLLAGTRPSDAATASAQPFTIGIMGGEIDGT
                     FMRIATDLTSVLNSNEMRIVPIVGKGSLQNMGDLLHLPGVDLALIAADVLAYAQNTHL
                     YPGELGKIQYICKLYDNDVHVCAGPDVQTLADLQGKPVNIDVEGAGTNLTARAVFRTL
                     GITPDFRTDEPTVGQDKLRRKLIAANVYLGGKPIRLFANTPADTGLHFVPVPSNEALE
                     KTYLPGGQLTHAEYPTLVPADEVVETVGVGVTLAVFGWQPASVRYRNLVTFVDRFFTS
                     FPELLKPPHHPAWHNVNLAAAQPGWVRFPPAQAWLAQHAPGAATPENADAKMRAQFDA
                     FLTQQGIPHLTAAQRDATWQYFQQQRLLGR"
     gene            1363..1917
                     /locus_tag="VGC82_06375"
     CDS             1363..1917
                     /locus_tag="VGC82_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEY0422934.1"
                     /translation="MKTFAKIVTGTLLATSGSIAAIAQTNAPLAYIQPMPPAAVQAVQ
                     EHLRGAGAYSGAADGVWGPDSANALQRFQANHQLQATGQLNQATATALGLDPASLLGT
                     QQAAVSPPVPPAEHLRPSSVRAIQERLRSLGFYTGGVDGVWGQSTQSAIQQFQQNRGL
                     QPNGQLNPATVTAIGIPQDSLAYR"
     gene            complement(1953..>2190)
                     /locus_tag="VGC82_06380"
     CDS             complement(1953..>2190)
                     /locus_tag="VGC82_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0422935.1"
                     /translation="QATLSLAAAIIAEASLSFLGLGQQPPSPSWGSMLNTAQRFLTQA
                     PWLAVFPGATIFLCVLSFNLVGDGLRDALDPRGR"
BASE COUNT          442 a          698 c          655 g          395 t
ORIGIN      
        1 attgatcccg atcccgcttt ggcgcgccaa tggtatgaac aagccgtcgc gatgggggat
       61 gtcgaggccg gtcctctcct tgaagccctg tccatgaggt aacgatgcac cggcgcaccc
      121 tgctgtcaac tcttcctgtc gccacgcttc tcgccggcac acgtccctcg gacgcggcaa
      181 ccgcgtcggc gcaaccattc acgatcggga tcatgggcgg cgagatcgac ggcacgttca
      241 tgcgcatcgc caccgacctg acctcggtac tgaactcaaa tgaaatgcgc atcgtgccga
      301 tcgtgggcaa gggctcgctg cagaacatgg gcgatctgtt gcatctgccg ggcgtggatc
      361 tggccctgat cgcggcggat gtcctggctt atgcgcagaa tacccatctt tacccgggcg
      421 agctgggaaa gatccagtat atctgcaagc tctacgacaa cgacgtgcat gtctgtgccg
      481 ggccggacgt tcaaaccttg gcggatctgc agggaaagcc ggtcaacatc gatgttgaag
      541 gtgccggcac caacttgacc gcccgcgccg tcttcaggac gttgggcatc acgcctgatt
      601 tccggaccga cgaaccaacc gttggacagg acaagctgcg gcgcaaactg attgccgcca
      661 acgtttacct cggtggcaaa cctatccgcc tgttcgccaa cacgccggcc gacaccggct
      721 tgcacttcgt cccggttcct tcgaacgaag cgctggaaaa gacctacctg ccgggcggcc
      781 agttgaccca cgcggaatac ccgacattgg ttccggccga cgaggtggta gaaacggttg
      841 gcgtcggcgt cacgctcgcc gtattcggct ggcagcctgc gtcggttcgc tatcgcaatc
      901 tggtcacgtt cgttgaccgg ttcttcacct cgttccccga actgttaaag ccgccacacc
      961 atccggcttg gcacaacgtc aatcttgccg ccgcgcaacc gggttgggtt cgcttcccgc
     1021 cggcgcaggc ctggttggcg cagcatgctc ctggcgccgc gacgccggaa aatgcggacg
     1081 ccaagatgcg ggcgcagttc gatgcgtttc ttacccagca gggaatcccg cacctgaccg
     1141 cggcacaacg ggacgcgacc tggcaatact tccagcaaca gcggcttctg gggcggtagg
     1201 gcgactggct gattaacggt ccgcggatgc tggcgcagtg cgcagcacaa ggagttgcgg
     1261 atacgtgaag gatcacgaca aaacgctgat caaaagatgg gtgcggcggt gaaccggtcg
     1321 gatgcagaag cgttttagcc cacacagtgg aggatgtgcg acgtgaaaac cttcgcgaaa
     1381 attgtgaccg gcaccctttt ggcaacatcc ggcagcattg ccgctatagc ccagacgaac
     1441 gcaccgctcg catatattca gcctatgcct ccagctgcgg ttcaggcggt tcaggagcat
     1501 ctccgcggcg ccggcgccta tagtggggcc gctgacggag tctggggccc ggacagcgct
     1561 aacgccctgc agcggttcca ggccaaccat cagctacagg caaccggcca attgaatcag
     1621 gcgaccgcca ccgcacttgg cctcgatccg gcctcgttgc tcggtacgca gcaggcagcc
     1681 gtttcgccac cggtcccacc agccgaacat ctgcggccgt cttcggtccg agccattcag
     1741 gagcgtctgc gcagtttggg gttctacacc gggggcgtgg atggagtctg gggacagagc
     1801 acgcaaagcg cgatacagca gttccagcag aatcgcgggc tgcagccgaa tgggcagctt
     1861 aatccggcaa ccgtcacggc tatagggatt ccgcaggact cgctcgctta cagataaatt
     1921 ggcttctgct caccgggaat cagggacttc cttcatcggc cgcgcggatc caaggcatcc
     1981 cgcagcccat cgccgaccag attgaacgac aacacgcata gaaaaattgt cgccccggga
     2041 aacaccgcca gccacggcgc ctgggtcaaa aatcgctgcg ccgtgttcag catcgatccc
     2101 cacgaaggcg atggtggctg ctgacccaga ccaagaaacg acaaggacgc ttcggcgatg
     2161 atcgctgccg ccagcgacag cgttgcctgc
//
LOCUS       DASWQN010000389         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1621253, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000389 DASWQN010000000
VERSION     DASWQN010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621253"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VGC82_06385"
     CDS             <1..341
                     /locus_tag="VGC82_06385"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HEY0422936.1"
                     /translation="QESLSQRVARATRLLSTRVDLTREQQNQALLASMNSRARMQLRL
                     QTTVEGLSVAAVTYYVSALVGHAAEALRTAGLHVEPNLATGISIPVVALIAWFGIHHI
                     RKTVTRPGNE"
     gene            complement(319..675)
                     /locus_tag="VGC82_06390"
     CDS             complement(319..675)
                     /locus_tag="VGC82_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422937.1"
                     /translation="MTHSGCYLRICQAAIGSLDQTNLAPKPFSKIQQMRDQRRNQRRR
                     DPRPRRTRQTHAQGKHEEHTWQQHQPDTDQTQGTNPESLENRQRDCLDQRQRQNHVNQ
                     VWRNKKRGKFTHYLAG"
     gene            1077..2558
                     /locus_tag="VGC82_06395"
     CDS             1077..2558
                     /locus_tag="VGC82_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HEY0422938.1"
                     /translation="MCQHAVVIGAGFGGLAAAIRLGARGYRVTIVEKLDAAGGRASVF
                     RQDGFNFDAGPTILTAPFLLEELWRLCGRELADDVTLTPVTPFYRIRFHDGSTFDCTG
                     DPDSMRREVARLSPGDVEGYEQFMRLSETTCRIGFEELGDVPFGSIADMIRIAPDLLR
                     LGGHRSVHAVVSRFIKDERLRIVFSFHPLLIGGNPFTASAIYTLIPYLERRWGVHFAM
                     GGTGAVITGLVNLIESQRSTIRYNSEVEQIMVANGRARGVRLISGETIPADIVVSNAD
                     SAWTYRKLLPPEARRRWTDRKLARARYSMGLFLWYFGTNRRYDEVAHHTILLGPRYRE
                     LLHDIFQRKVLAEDFSLYLHRPTATDPSSAPTGCDAFYVLSPVPNLSGKTNWRTAAEP
                     YRQAIASHLSATLLPDLERHMVTSKLMTPLDFQDRLCAWQGAAFGLEPVLLQSAWFRP
                     HNRSEDIDGLYLVGAGTHPGAGVPGVLSSAKVLDRLVPHAAVR"
     gene            2542..>3439
                     /locus_tag="VGC82_06400"
     CDS             2542..>3439
                     /locus_tag="VGC82_06400"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEY0422939.1"
                     /translation="MPLFADSEADDLNACRTLLRGGSKSFDAAARLLPRSVRDPAIAL
                     YAFCRDADDTIDAATGSLDRLRERLALAYEGRPLATPSDRAFARVVERFAMPRALPDA
                     LLEGFGWDEEGRRYRDLSELRAYAVRVAGTVGAMMAVLMDVRDPPRLAAAIDLGVAMQ
                     LSNIARDVGEDARNGRLYLPLDWLAEAGIDPDAFLARPQYSAALGAVVRRLLDAADAH
                     YRQATAGIVHLPPRCRLGIGAAQLLYAEIGREVARRGMDSVSARAVVSGRRKIGVLAA
                     GLPGMALKPRPLPRACHDGRYLP"
BASE COUNT          555 a         1080 c         1089 g          715 t
ORIGIN      
        1 gccaggaatc gctgtcgcaa cgagtcgctc gcgccacgcg gttgctgtca acccgggtgg
       61 atttgacgcg cgagcagcag aaccaggcat tgctggcatc gatgaacagc cgggccagga
      121 tgcagttgcg gttgcagacg acggtggagg ggctgtcggt ggcggcggtc acctattacg
      181 tctccgccct ggtcggccat gcagcggagg ctttgagaac cgccggcttg cacgtcgaac
      241 ccaatttggc cacgggtatc agtatcccgg tcgtcgcgct gatcgcgtgg ttcgggatcc
      301 atcacatccg caaaacggtt acccggccag gtaatgagtg aatttgccgc gttttttgtt
      361 tcgccacacc tggttgacat ggttctggcg ctgacgctgg tcgaggcagt cgcgctgacg
      421 attttccaga gattcagggt tggttccctg cgtttggtcg gtgtctggct gatgctgctg
      481 ccaggtgtgt tcctcatgct tgccctgcgc gtgggtctgg cgggtgcggc gtggccgtgg
      541 atcccggcgg cgctggttgc ggcgctgatc acgcatttgc tggatcttcg agaacggctt
      601 cggggctaaa ttcgtctggt ccaggctgcc gattgcagcc tggcagatgc gaaggtaaca
      661 accggagtgc gtcatttcca tatcctttgc attgcggacg ccactaatta ctgctcgtga
      721 ccctcctgct tttcattagc gctacactcc tggatacact ggcgcgtgat gagatagttt
      781 caccacgaca cttcctcggt atgatgttgt tggtatggcc tcatgttcat tggcagaaga
      841 ttgccatgta ggctcgtcat gacgatagct gtgcaaagtc ggtaactatg ctcggcgacg
      901 gtcctgaccg tctggacgca aggatcattt acgccggaca ggcaacccat gtcgcgggtc
      961 cttgccggtt caagctcgtc aatgccctct ggtaagactg tcatctaacg tttacactca
     1021 gccgcatgga cgcgttgaca ccctctcgca tcgacctgcc gccagatccg cccggcatgt
     1081 gccagcacgc cgtggtgatc ggtgcaggat ttggcggcct cgcggcagcc attcgattgg
     1141 gcgcgcgggg ctaccgtgtc actattgtag agaagctcga tgccgccggt ggccgagcgt
     1201 cggtgttccg tcaggacgga tttaacttcg atgccggccc caccatcctg actgctccat
     1261 tcctgctgga ggaactctgg cgcctgtgcg gccgcgaatt ggcggatgat gtgacgctga
     1321 ccccggtcac accgttttat cgcatccgct tccacgatgg atccacgttc gactgcacgg
     1381 gcgaccccga ctccatgcgg cgggaagtcg cccgtttgtc ccccggcgat gtcgaaggct
     1441 atgagcagtt catgcggctg agcgagacca cttgccgcat cgggttcgag gaacttggcg
     1501 atgtcccctt cgggtccatc gccgacatga tccggatcgc gcctgacctg ctgcgtctgg
     1561 gcggacaccg cagcgtccat gccgtcgtca gccgtttcat caaggacgaa cgcctgcgga
     1621 tcgtcttcag cttccatccg ctgctgatcg gcggcaaccc gtttaccgcc agcgccatct
     1681 acacgctgat cccttatctg gagcggcgct ggggcgtgca tttcgccatg ggcggcaccg
     1741 gtgcggtgat aaccggcctg gtcaacctga tcgaaagcca gcgcagcacg atccgctaca
     1801 actcggaagt cgagcaaatc atggtggcca acgggcgtgc tcgcggcgtc aggctgatat
     1861 ccggggaaac cattccggcc gacatcgtcg tgtccaacgc cgattccgcc tggacctatc
     1921 gcaagctgtt gccgcccgag gcacgccggc gttggacaga ccgcaagctt gcgcgcgcgc
     1981 gctactcgat ggggctgttt ctttggtatt tcggcaccaa tcgccgctac gacgaggtgg
     2041 ctcaccatac gatccttctt ggcccgcgtt atcgcgaact gctgcacgac attttccagc
     2101 gcaaggtgct ggccgaggat ttttcgttat acctgcaccg gccgacagcg accgatccat
     2161 cctcggcacc cacgggctgc gatgcctttt atgtgctgtc gccggtgccg aacctgagcg
     2221 gcaagacgaa ttggcggact gccgccgaac cgtatcgtca ggccatcgca tcgcatctgt
     2281 cggcgaccct cctgcccgat ctggaacgtc acatggtcac atcgaagctg atgacgccgc
     2341 tcgatttcca ggatcgcctg tgtgcctggc aaggcgctgc attcgggctg gaaccggtgc
     2401 tgctgcaaag tgcgtggttt cgcccgcaca accgcagcga ggacatcgac ggactctatc
     2461 tcgtcggtgc cggcacgcat ccgggagccg gagtgccggg tgtgctgtca tccgccaagg
     2521 tcctcgatcg tctggtgcca catgccgctg ttcgctgatt cagaagccga tgacctgaac
     2581 gcctgccgaa ccctgctgcg cggcggatcc aaaagctttg acgcagcggc aaggctgctg
     2641 ccacgcagcg tgcgggatcc ggccatcgcg ctgtacgcat tctgccggga tgcggatgac
     2701 acgatcgatg cggccaccgg cagcctggac cggcttcgag agcggctggc cctggcatac
     2761 gaaggccggc cattggcaac gccgtccgac cgtgcctttg cccgcgtcgt ggaacgtttt
     2821 gcgatgcctc gcgcgctgcc cgacgccctg ctggaagggt tcggctggga tgaggaaggc
     2881 cgccgctacc gggacctatc cgaattgcgg gcctacgcgg tacgtgtcgc cggcacggtc
     2941 ggggcgatga tggcggtgct gatggacgtg cgcgacccgc cccggctcgc cgcggcgatc
     3001 gatctcggcg tagcgatgca gttatccaat attgcccgcg acgtaggtga ggatgcgcgc
     3061 aacgggcggc tttatctgcc gctcgattgg ctcgccgagg ccggcatcga cccggacgct
     3121 ttcctcgccc gcccccaata ctccgctgcc ctcggcgcgg tggtgcggcg actgctggac
     3181 gccgcggatg cgcattaccg ccaggccaca gccggcatcg tccacctgcc gccacgttgc
     3241 cgcctcggca taggcgccgc gcagttgctc tacgctgaga tcggacggga ggtggcgcgt
     3301 cgcggcatgg actcggtgtc tgcccgagct gttgtttccg gtcggcggaa gataggcgtg
     3361 ctggccgcgg gcctgcctgg aatggcactg aagccgcggc cgttgccccg cgcgtgccat
     3421 gacggaaggt atcttcctt
//
LOCUS       DASWQN010000390         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_565546, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000390 DASWQN010000000
VERSION     DASWQN010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565546"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3009)
                     /locus_tag="VGC82_06405"
     CDS             complement(<1..>3009)
                     /locus_tag="VGC82_06405"
                     /inference="COORDINATES: protein motif:HMM:NF045168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422940.1"
                     /translation="LCGYPNLADKKAPFGDETNRVHTLLRDGSFLAVRKLRQDVEALE
                     ETLYHATRQAADAAEPGATTPLLTRETLLAKMMGRWPNGHPRVGQPLTRVPPSDPLLN
                     DFNYDYDAQAEQCPFHAHIRRANPRVRITRADGGARPPRIVRRGMSYGPPVGSGDDVQ
                     TAQDALQQERGLVFMAYNASLGEQFEVVQRWLAGGNSSGSHSGESDPFLGLAEPGRLR
                     HFRFEHEGQTVRMELDGSDRLHDEPRPFVRLEWGEYFFAPSKKALASLQGWAANRYRK
                     PQVTWSVDAGEKEIARLREFESRHGDVEAKAAWKTALEDPDSATHFINASIWAAIRYR
                     HGGVLRTPFGVLVADRDMVQQVFADPGRNLTITGYLPRMEQSFGILYLGRDAGQQDHA
                     YEQESEASNTAILALDQPSAFELARATTEKVLEDLVEQTKGYAQEDGEASWELTVDVR
                     ELLEPLLAEFCEEWFGLSKDEGYFCRAGYRWDWKPGQPPNYPGHFLSPSRYIFQPHPN
                     QEVTAVGSAHGAAVRHAMIGFLNRFGTTIRAPVTRAVLDSPRAAGDIPFVARTVAGAM
                     MGFIPTVEGNLRRILNEWLREGTLWSLRARYAGTPKPNFTDALNRLRDDFIPAMQLRA
                     VPELIWRTAVTPHTLGAGEHQVAVKPGDIIVAGAVSATQQNLSEGRQDLYHAFGGNRR
                     VAGHPTHSCPGADPALAVMLGFFSALVESPLRLRAGPAPLTLSVDGRLPGLPGSPLTL
                     ALETRMLTANDFRSFRSSPAGTLHDPQAYQIKDADKRLTTTAIPLATLGDSWLFQFPP
                     IIFRPSLTSSLKTLGYVVGTSHRFGTPGARLQQMASASSLEDLRNCFRNPDPSEPAPQ
                     ALLIGGGGNDVVYRYNIAPKESPLYKMLRPAPQPGEDLLIEEVVHAFIDVIIANFYRA
                     ILNCVVHELKDFTNIPILIHGYDHSIPDGRKDPASGPWLAPIFSAAGMTDPTVSREVV
                     RRLIDRVGKMVATVAAEYPGRVFPVATAGKL"
BASE COUNT          528 a          914 c         1008 g          559 t
ORIGIN      
        1 agcttgccgg cggtggcgac cggaaacacg cggcccggat actcggctgc gacagtcgcc
       61 accatcttgc cgacccggtc gatcagacgt cgcaccactt ctcgtgacac ggtcgggtcg
      121 gtcatgccgg cggcgctgaa gattggcgcc agccacgggc ccgacgccgg gtcctttcgg
      181 ccatcgggaa tcgaatgatc gtagccatgg atcaggatgg gaatgttcgt gaagtccttt
      241 aactcgtgga cgacgcagtt gaggatggct cggtagaagt tggcgatgat gacgtcgatg
      301 aacgcgtgaa ccacctcctc gatcagcagg tcttcgccgg gctgcggcgc cgggcgcagc
      361 atcttgtata acggcgattc cttcggtgcg atgttgtagc gataaaccac gtcatttccg
      421 ccaccaccaa tcagcagcgc ctgcggtgcc ggctcggagg gatcgggatt tctaaagcag
      481 tttctcaaat cttccaggct gctggcgctg gccatctgct gaaggcgtgc gccgggcgtg
      541 ccgaagcgat ggctggtacc gacgacgtag ccgagagtct tgagcgatga tgtcaggctc
      601 ggccgaaaga tgatgggcgg gaattgaaac aaccaggaat cgccgagcgt cgcgagcggg
      661 atcgccgtcg ttgtcaatcg cttgtcggcg tccttgatct gataggcttg ggggtcatgg
      721 agcgtgccgg caggcgacga acggaatgaa cggaaatcgt tggcggtcag cattctggtc
      781 tccagggcaa gggtgagggg agagccgggc agccccggca gccttccgtc gacgctcaga
      841 gtgagcgggg cgggaccggc tcgcaaccgc agcggcgatt ccaccaatgc gctgaagaaa
      901 cccagcatca cggccagtgc cggatctgct ccgggacacg aatgggtggg atgacccgcg
      961 accctgcggt tgccgccgaa ggcatgataa aggtcctgac ggccttcact gagattttgc
     1021 tgggtggcgg agaccgcacc ggcgacgatg atgtcccccg gcttgaccgc aacctggtgt
     1081 tcgcccgctc ccagcgtgtg cggagtgacg gcggtccgcc atatcaattc gggcaccgcg
     1141 cgcagctgca tcgccgggat gaagtcgtcc cgcagccggt tgagggcatc ggtgaagttt
     1201 ggcttcgggg tgccggcata gcgcgcgcgc aacgaccaca gagttccttc gcgcagccat
     1261 tcgttcaata tccggcgcag attgccttcg acggtcggaa tgaagcccat catggcgccg
     1321 gcgacggtgc gcgccacaaa agggatgtcg ccggcggcgc gtggagagtc gagaaccgcc
     1381 cgcgtgacag gtgcacggat cgttgtgccg aaccggttga ggaaaccgat catggcgtgg
     1441 cgtaccgccg cgccatgggc ggaaccgacc gccgtcactt cctgatttgg atggggctgg
     1501 aagatgtagc gtgagggtga aaggaaatgc ccgggatagt tcggtggctg acctggcttc
     1561 cagtcccagc gatagccggc gcggcagaag taaccctcgt ccttactcag tccgaaccac
     1621 tcttcgcaga attcggccag cagcggttcc agcagttcgc gcacgtcgac cgtcagctcc
     1681 cagctcgctt cgccgtcttc ctgggcataa cctttcgtct gctcgaccag atcttccagt
     1741 actttctccg tcgtcgcccg cgccagctcg aacgcagatg gctggtcgag ggccaggatc
     1801 gcggtattgc tggcttcgga ttcctgctcg tacgcgtggt cttgctgtcc ggcgtcgcgg
     1861 ccgagataga gaatgccgaa cgattgttcc atgcggggga ggtagccggt gatcgtcagg
     1921 ttacggccgg gatcggcaaa tacctgctgg accatgtccc ggtcggccac caacacgccg
     1981 aagggcgtac gcagcacgcc gccgtgccga tagcggatcg ccgcccagat cgatgcattg
     2041 atgaaatgcg tggccgaatc cggatcctcg agcgcggttt tccacgctgc cttcgcctcg
     2101 acatcgccgt gccggctctc gaattcgcgc aggcgtgcga tctccttttc gccggcgtcc
     2161 acgctccacg tcacctgcgg cttgcgatag cggttcgccg cccatccctg caaactcgca
     2221 agcgcctttt tggagggcgc aaagaaatac tcgccccatt ccaggcgtac gaacgggcgg
     2281 ggttcgtcgt ggagccggtc cgaaccgtcg agctccatgc ggacggtctg gccttcatgc
     2341 tcgaaacgga aatgccgaag ccggcccggt tcggcgagac cgaggaaggg gtcgctttcc
     2401 cccgaatggg agccggagct gttgccgccg gccagccagc gctgcaccac ctcgaactgt
     2461 tcgccgaggc tggcgttgta agccatgaag acgaggccgc gttcctgttg caaggcatct
     2521 tgggcggtct gcacgtcgtc gccggatccg acgggtgggc cgtaggacat gccgcgtcta
     2581 acaatacggg gcgggcgggc gccgccatcc gcgcgcgtga tccgcacccg cggattggca
     2641 cgacgtatgt gcgcgtgaaa aggacactgt tcggcctgcg cgtcgtagtc gtaattgaag
     2701 tcgttgagca gcggatcgga tggcggaacc cgggtcagcg gctggcccac cctcggatgg
     2761 ccgttgggcc agcggcccat cattttggcc agaagcgttt cgcgcgtcag taacggggtg
     2821 gtggcgccgg gctccgccgc gtcggccgcc tgtcgcgtgg cgtgatagag ggtctcctcg
     2881 agcgcctcga catcctgtcg cagtttgcgt accgccagaa agctgccgtc gcgaagcagc
     2941 gtgtgaacgc gattagtttc gtcgccgaac ggggccttct tgtcggccag gttcggatag
     3001 ccgcacaag
//
LOCUS       DASWQN010000391         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1621332, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000391 DASWQN010000000
VERSION     DASWQN010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621332"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VGC82_06410"
     CDS             complement(<1..1175)
                     /locus_tag="VGC82_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0422941.1"
                     /translation="MAEFDYIIVGAGSAGCVLANRLSEDNRSTVLLLEAGPKDSSVWI
                     NIPAGFTKLLNNPKYNWNFESEPEPNTANRRIPIPRGRTLGGSSSINGMLYVRGNPLD
                     YNTWSQFGNRGWGYDSVLPYFRKAENFAPGGDASRGRGGPLNVEHMRERAELLDAFID
                     AAVDQGYPRNKDYNNGHQEGFGYFQVTQKNGERWSCARGYIDPIRGRPNLKIETEAYA
                     TKVMLDGKRAVGVAYIQGGVTKEARANREVIIAAGAVKSPHILELSGIGNPDILSPLG
                     IQVAHALPGVGENYRDHYAPRMNWRVKLPVTLNERSRGIALAKEVFKYYTQRRGILTF
                     TAGIAFGFVRTRPELEEPDVQFHFAHASYGNAQKRDLETEPGMTLTVYQCRPESKGS"
     gene            1277..>2503
                     /locus_tag="VGC82_06415"
     CDS             1277..>2503
                     /locus_tag="VGC82_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006698400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEY0422942.1"
                     /translation="MGNAARFSFLSLLRHGAGKAWDKQWRDAQPKASYDVVIVGAGGH
                     GLATACYLAKEHGIRDVAVLDKGWLGGGNTGRNTTIIRSNYLQPESAMLYEHALKRWE
                     TLSQDLNYNVMYSPRGVLMLAHTHHDVLVFKRHVHANRMQGIDNEWLTPAQAKTFCPP
                     LDVASDARYPVLGAALQRRGGVARHDAVAWGYARAASAMGVDIIQNCAVTGIVRDATG
                     AVAGVETTRGTIASGRVGVAAAGHTSVVMAMAGVRMPLESFPLQALVSEPVKPVLPCV
                     VMSNTVHAYVSQSDKGELVIGGGTDIYTSYSQAGGLHVACETLAAICELFPSFRRLRM
                     LRSWGGIVDTTPDRSPIIGLTPVPGLYVNCGWGTGGFKATPGSGHIFAATIARGEPHP
                     IAAPFSLDRFVTGRLID"
BASE COUNT          419 a          766 c          832 g          486 t
ORIGIN      
        1 atcgaccctt ttgactccgg gcggcactga tacaccgtca gcgtcatgcc gggttcggtc
       61 tccaggtccc gcttctgggc attgccgtag ctggcatggg cgaagtggaa ctgcacgtcg
      121 ggctcctcca gctccggccg ggtcctgacg aagccgaatg cgatgcccgc cgtaaaggtc
      181 aggataccgc gccgttgggt gtagtatttg aacacctcct tcgccagggc gatgcctcgg
      241 ctgcgctcgt tcaacgtgac cggcagcttc acccgccagt tcatccgcgg cgcgtagtgg
      301 tcgcggtagt tctcgcccac acccggcagc gcatgggcga cctggatacc gagcggggac
      361 aggatatcgg ggttgccgat gccggacagt tccagaatgt gcggcgactt tactgcgccg
      421 gcggcaatga tcacctcccg gttggctcgg gcttccttgg tgactccacc ctggatgtag
      481 gcgacgccga cggcgcgctt gccgtccagc atcactttcg tcgcgtaggc ctcggtttcg
      541 atcttgaggt tcgggcggcc gcggatggga tcgatatagc cacgggcgca agaccagcgc
      601 tcaccatttt tctgcgtaac ctggaagtag ccgaagccct cctggtggcc gttgttgtag
      661 tccttgttgc ggggatatcc ctgatccacc gccgcgtcga tgaacgcatc cagcagttcg
      721 gcgcgttcac gcatgtgctc cacgttcaat gggccgccgc ggccgcgcga ggcgtcgccg
      781 ccgggcgcga agttctctgc cttgcggaag tacggcagta cggagtcgta gccccagccg
      841 cggttcccga actgcgacca tgtattgtag tccagaggat tgccgcggac atacagcatg
      901 ccgttgatgg aactggaccc gcccagcgtg cgtccgcgcg gaatggggat ccggcggttg
      961 gcggtgttcg gttccggctc gctttcaaag ttccagttgt atttcgggtt gttgagcagc
     1021 ttggtgaagc cagcgggaat gttgatccac accgaactgt ccttgggtcc cgcctccagc
     1081 agcaggaccg tggaacggtt gtcctcgctc agacggttcg ccagcacgca gccggcggaa
     1141 ccggcgccca cgatgatgta gtcgaattcg gccatctcgt atcccccgct tgtggctagt
     1201 ctatacaatc aggccggggc cggtggaagg ctcgccgctt tgccttgaac ctggggtgtg
     1261 gcaaggatgc cgcgcgatgg ggaacgcagc acgattctcc tttctgtccc tgttgcggca
     1321 tggggccgga aaggcatggg acaagcagtg gcgggacgct cagcccaagg cgtcgtacga
     1381 cgtggtgatc gtgggcgccg gcggacacgg tctggcaacc gcctgttacc tggcgaagga
     1441 gcacggcatc cgcgacgtcg cggtgctgga caagggctgg ctcggtggcg gcaacaccgg
     1501 gcgcaacacc acgatcatcc ggtcgaacta tcttcagccg gaaagcgcga tgctttacga
     1561 gcacgcgttg aagcgctggg aaacactgtc gcaggacctg aattacaacg tgatgtactc
     1621 gccgcgcggc gtcctgatgc tggcgcacac gcatcacgac gtacttgtgt tcaagcgcca
     1681 tgtgcatgcc aaccggatgc aaggcattga caacgaatgg ctgacacccg ctcaggcgaa
     1741 aacgttttgt ccgccgctgg acgtcgcttc cgatgcccga tatccggtgc tgggagcagc
     1801 cttgcagcgc cgtggcggtg tcgcccgaca tgacgccgtg gcctggggtt atgcgcgggc
     1861 ggcctccgcg atgggtgtgg atatcatcca gaattgtgcg gtgaccggca tcgttcgcga
     1921 cgccaccggg gcggtcgccg gcgtcgaaac cacccgaggc acgatcgcgt cagggcgggt
     1981 tggcgtggcg gcggccggtc ataccagcgt ggtgatggcg atggcaggcg tgcggatgcc
     2041 gctggagtcg ttccccctgc aggcattggt cagcgagccg gtgaaaccgg tgctgccgtg
     2101 cgtagtgatg tcgaacaccg tgcacgccta cgtttcccag tccgacaaag gcgaattggt
     2161 gatcggtggc ggaacggaca tctatacatc ttatagccaa gccggtgggc tgcacgtcgc
     2221 ctgcgagaca ttggcagcaa tctgcgagct gtttccatcc ttccggcgtc tgcggatgct
     2281 gcggagctgg ggcggcatcg tcgataccac accggaccgc tcgccgatca tcggcctgac
     2341 gccagtgccg ggattgtacg tcaactgcgg ctggggaacg ggtgggttca aggcgactcc
     2401 gggttccggc cacatattcg ccgcgacgat cgcgcgcggg gagccgcatc cgatcgcagc
     2461 accattcagc ctggaccgct tcgtcaccgg ccgcctgatc gat
//
LOCUS       DASWQN010000392         1696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_38359, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000392 DASWQN010000000
VERSION     DASWQN010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38359"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VGC82_06420"
     CDS             complement(<1..815)
                     /locus_tag="VGC82_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0422943.1"
                     /translation="MGKLAGKVAIVTGASRGIGQQIAELFASEGAKVVCAARTLNEGE
                     HRMLEGSLSRTVELIRAKGGEATAVTADVSSEAECVALVQAARAAYGPIDILVNNAAL
                     NYYLPTETYQTNRWVRCFAINVHAPFILAKEVLTDMIPRRSGAIVNISSGAAIGPGRG
                     PYDDRTVRGGVMYGATKAALERFTQGLAQEVAQYDGISIAAVSPSQVVPTPGTIYHKL
                     VTGIDDPKGEHPILMARAVLLLASEAPLKVNGRVTYSQQILKEFGWIDDAKGRG"
     gene            1001..1498
                     /locus_tag="VGC82_06425"
     CDS             1001..1498
                     /locus_tag="VGC82_06425"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY0422944.1"
                     /translation="MMKQLSITLGALAIGFAATQAMAQMSPADKTFATKAASGGMAEV
                     QLGRLAAQNAGSPAVREFGQKMATDHTQANQALQEIARQQQLTLPTAPASADQATEER
                     LRAMKGNAFDTAYMKDMVQDHRQDVADFRKEAESGTDPALKAFAQKYLPVLEQHLKMA
                     ETAAK"
BASE COUNT          324 a          590 c          499 g          283 t
ORIGIN      
        1 ccccggcctt tcgcatcgtc gatccagccg aactccttca gtatttgctg gctgtaggtc
       61 acgcgcccgt tcaccttcag cggcgcttcg ctggccagca gcaaaacagc ccgagccatc
      121 aggatcggat gctcgccctt cggatcgtcg atgccggtca ccagcttatg gtagatcgtt
      181 ccaggtgtgg gaaccacctg cgacggagaa accgccgcga tgctgatccc atcgtactgt
      241 gcgacctcct gcgccaggcc ctgggtgaag cgctccagcg ccgccttggt cgccccgtac
      301 atgacgccgc cgcgcacggt ccggtcgtcg tacggccctc gtcccggtcc gatcgccgcc
      361 ccggaactga tgttgacgat ggcgcccgac cggcggggga tcatgtcggt cagcacctcc
      421 ttcgccagga tgaacggagc atgcacgttg atcgcgaagc agcgcaccca gcgattggtc
      481 tgataggtct cggtcggcag gtagtagttc agcgcggcat tgttcaccag gatgtcgatc
      541 ggaccatagg cagcccgggc cgcctggacc agcgcaacgc attccgcttc cgaggagaca
      601 tcggccgtta ccgcggttgc ctcgccaccc tttgcgcgga tcagttcgac ggttcgcgac
      661 agcgagccct ccagcatccg gtgctcgccc tcattcaacg tgcgcgccgc gcagaccact
      721 tttgccccct cggaggcaaa cagttcagcg atctgttggc cgatgccccg gctggccccg
      781 gtgacgatag caacttttcc ggcaagcttt cccatgtcca tttccttatc ccgacggagc
      841 gttgacacgc agccgcgtca agggcggtag gcccgttatt gctttagccc gcagccgcca
      901 caccaacccg agcgcacacc aacgccatca tccgggtgaa gcggcacccg cgcaaccgcc
      961 cggtacaaga tgcgttccga ctgaaccaca ggaggaaccc atgatgaagc agctctcgat
     1021 caccctcggc gcattggcga tcggtttcgc cgccacccag gccatggcgc agatgagccc
     1081 cgccgacaag acctttgcca ccaaagccgc atccggcgga atggccgagg tccagctcgg
     1141 gcgtctggca gcgcagaacg cgggatcgcc ggcggtgcgt gaattcggtc agaaaatggc
     1201 caccgaccat acccaggcca atcaagcatt gcaggaaatc gctcggcagc agcagttgac
     1261 gttgccgacc gcgcccgcat ccgccgacca agccacggaa gagcggttgc gcgcgatgaa
     1321 gggcaacgcc ttcgacaccg cctacatgaa ggacatggtg caagaccatc gccaggacgt
     1381 ggccgatttc cgcaaggaag ccgagagtgg aaccgacccc gcgctgaagg ctttcgcgca
     1441 gaaatacctc cctgtgctgg agcagcacct gaagatggcc gaaaccgccg ccaagtgatc
     1501 tccggcgccc atgtgacctc aggcctccaa tgacctcagg cgctcatctg acctcaggcg
     1561 ctcatctgac ctcaggcggg cgcgcgaacg aacgggttgc gcaggacgcc gatgcccgtc
     1621 agttcgacct cgacgacatc cccatgcacg aggtcgggtg acgtgccatc ggtgcccatc
     1681 cagatgacgt cgcccg
//
LOCUS       DASWQN010000393         2870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1488992, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000393 DASWQN010000000
VERSION     DASWQN010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488992"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VGC82_06430"
     CDS             <1..763
                     /locus_tag="VGC82_06430"
                     /inference="COORDINATES: protein motif:HMM:NF020939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HEY0422945.1"
                     /translation="LEEMVALTEAVTCRTRSLLTCFGEALACDCGHCDNCEAPPITVD
                     ASTEAQMVLSAVYRTDQIFGAAHIVAVLRGEKTEGVLRHRHDSLRTFGVGAAHSGVYW
                     RGLIRQLIALRALDVDTQGHGGLFLVQEEVRPILRGEVRVMLRQDQPRPVRTDRVERG
                     RPVAVPAVGGGEQADPSLYEALRLWRLAEAKGQSIPPYVIFHDSVLREIAALRPATLD
                     ALAQIKGVGASKLERYGRLVLGVLRSQVPDGLKRG"
     gene            complement(912..2387)
                     /gene="zwf"
                     /locus_tag="VGC82_06435"
     CDS             complement(912..2387)
                     /gene="zwf"
                     /locus_tag="VGC82_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY0422946.1"
                     /translation="MPKIIPVAIFDCVVFGATGDLTLRKLLPALYYRFRDGQMPAESR
                     IIGAARSRLSDDEFRQRAAQALESHVAPADQDADTMRRFREQLHYVSIDATAPDADWT
                     RFDTVLDDARIRVFYLATSPDLYGPICRALGTNGMVTDQSRVVLEKPIGHDAESAHAI
                     IEEVGKVFTEAQTFRIDHYLGKETVQNLMALRFANTIFERLWTADVIDHVQITVAETV
                     GLEGRGGYYDKSGALRDMLQNHMLQLLCLIAMDPPVSLEADRVRDEKLKVLRALKPME
                     PHEVAALTVRGQYAAGAIDGKPVPSYVTDLGANDASRTESFVALKAEVRTWRWAGVPF
                     YLRTGKRLPTKVSEIVIQFRAPPFSIFPPEAAEWRPNSLIIRLQPEEGMRLEMMTKDP
                     GPGGLRLSPTGLDISFEKTFGKRFPDAYERLLMDTVRGNPTLFMRHDEVEAAWAWVEP
                     ILEAWAARPDHPRPYPAGSWGPTAAIALIERDGRTWHEEME"
BASE COUNT          488 a          895 c          954 g          533 t
ORIGIN      
        1 gctggaggaa atggtcgccc tgaccgaagc ggtgacgtgc cgcacccggt cattgctgac
       61 ctgcttcggt gaggcgctgg cctgcgattg cggtcattgc gacaactgcg aagcgccccc
      121 gatcaccgtg gatgcctcga ccgaggcaca gatggtgctg tcggcggtct atcgcacgga
      181 ccagattttc ggcgccgcgc atatcgtggc cgtgctgcgc ggcgagaaga ccgagggggt
      241 actgcggcac cggcacgact cgctgcgcac gttcggggtt ggggcagcgc atagcggcgt
      301 gtactggcgg ggcctgatcc ggcagttgat cgcgctgcgg gcgttggacg tcgataccca
      361 gggtcacggc ggcctgttcc tggtgcagga ggaggttcgg ccgatcctgc ggggtgaggt
      421 ccgcgtcatg ctgcgacagg atcagcctcg tcccgtgcga acggaccggg tggagcgtgg
      481 gcgaccggtg gcggtgccgg cggttggcgg cggtgagcaa gcggacccat ccttgtatga
      541 ggcgctgcgg ctttggcggc tggccgaggc aaagggacag tcgatcccac cctacgtcat
      601 cttccacgat tcggtgctgc gagagatcgc cgcgctgcgg ccggcgacat tggatgcgct
      661 ggcgcagatc aagggagtcg gggcttccaa gctggaacgc tatgggcggc tggtgttggg
      721 ggtattgcgg tcgcaggtgc cggacgggtt gaagcggggg tgaggctatt gccccttcgt
      781 cgggcacgaa catcgccgtg atctcgtgca tctatccggc attgccgtat gtgctgcgag
      841 tgtggaaatc agcttcggcc cggcgagccg ccatcttcct tgatggttcc cgcagcgatc
      901 agccgcggcg gctactccat ttcctcatgc caggttcgtc catcccgctc gatgagcgcg
      961 atcgccgccg tcggtcccca gcttcccgcc ggatacggac gcggatggtc cggccgagcg
     1021 gcccaggctt ccaggatcgg ttcgacccag gcccaggccg cctcgacttc gtcatggcgc
     1081 atgaacaatg tcggattgcc gcgcacggta tccatcagca accgttcgta tgcgtccgga
     1141 aagcgcttgc caaaggtctt ttcgaagctg atgtccaggc cggtcgggga aagccgcagg
     1201 ccgcctggac caggatcctt ggtcatcatc tcaagccgca ttccttcctc gggctgcagg
     1261 cggatgatca ggctgttcgg gcgccattcc gccgcttcgg gtggaaagat cgagaacggc
     1321 ggtgcgcgaa actggatgac gatttcggaa acctttgtcg gcagccgttt gccggtgcgc
     1381 aggtagaacg gtacgccggc ccagcgccag gtacgcactt ccgccttcag ggcaacaaag
     1441 ctctcggtgc gagacgcgtc gttcgccccc agatccgtca cgtagctggg caccggcttc
     1501 ccatcgatgg ccccggcggc gtactgcccg cgcacggtca gtgccgcgac ctcatgcggc
     1561 tccatcggct tcaatgcgcg caagaccttc agtttttcgt cgcgcacccg gtccgcctcg
     1621 agcgacaccg gcgggtccat cgcgatcagg cagagaagtt gcagcatgtg gttttgcagc
     1681 atatcgcgca gcgcaccgga tttgtcgtag tacccgcccc taccctccag tccgacggtt
     1741 tcggcgacgg tgatctgaac gtggtcgatc acgtcggccg tccaaaggcg ctcgaagatc
     1801 gtgttggcga agcgcagagc catcaggttc tgcaccgttt ccttgcccag gtagtgatcg
     1861 atgcggaacg tctgcgcttc ggtgaacact ttgcccacct cttcgatgat cgcatgggcc
     1921 gattccgcgt catggccgat cggtttctcc agcaccacgc gagactggtc cgtcaccatg
     1981 ccgttggtgc ccaacgcccg gcagatcggg ccatacaggt cgggcgaggt cgccaggtag
     2041 aagacgcgaa tgcgggcgtc gtccagcacc gtgtcgaagc gggtccagtc cgcgtcgggc
     2101 gcagtggcgt cgatcgatac ataatgcagt tgctcgcgaa aacgccgcat cgtgtccgcg
     2161 tcctgatcgg cgggagcaac gtgactttcc agcgcctggg cggcacgctg gcgaaactca
     2221 tcgtccgaca ggcgggatcg ggcggcaccg atgatccggg attcggccgg catttgccca
     2281 tcgcggaagc ggtaatacag cgccggcagc agcttccgca gcgtcaggtc gccggttgcg
     2341 ccgaagacga cgcaatcgaa gatggcgaca ggtatgattt tcggcatcaa gctctcctgg
     2401 tcgatgatcc gttgccggat cttcgggggt gcgagcggat tgctacacca agctgcccgc
     2461 ggcggccacc gcctctgtca ggcgccaacg acaaccgcat gaccgaagga acccggcctt
     2521 cgctgcaggg attcgcggtc atgtttcgcg gcccggttag cctgatatca gggcgaatgc
     2581 gatcaggtcc agtcactcgg cccatgctgt cgtgcggcac gagacctgac gatcccaggg
     2641 cggttcatgc taaagtgaaa acacgggccg agcgggattg ccgcaatcga ccagccgacg
     2701 atggaaggga acccagcatg agcgaggtcg ccgttttcga catgctcggc aactcgttca
     2761 ttcggctggt gcagatgaac caaggcgctg gcatcgaaac caccacacgt cccggaccgt
     2821 ttccaagcgc ctgatgcaca atccgccgcc acggcgcttc cgcgaactgc
//
LOCUS       DASWQN010000394         4075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1357592, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000394 DASWQN010000000
VERSION     DASWQN010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357592"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VGC82_06440"
     CDS             <1..276
                     /locus_tag="VGC82_06440"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEY0422947.1"
                     /translation="REAADARDDAVKAGVTINGLAIINDHPVSWTFAHVQPPGGLANY
                     YRENVTGGPGNFVLEVHDFASFGEAMTRKLVDEIAAVPPLSNYATRR"
     gene            292..1287
                     /locus_tag="VGC82_06445"
     CDS             292..1287
                     /locus_tag="VGC82_06445"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0422948.1"
                     /translation="MGLLINGEWQEHEPETTKDGHFERRDTAFRNWITPDGRPGPTGQ
                     DGFRAAAGRYHLYVSLACPWAHRTLIMRALKGLQTIIPVSVTHWLMAENGWTFTTGNG
                     VIPDPLYGSRYLYELYARADETYSGRSTVPILWDQHTQTIVNNESADIIRMLGSAFDH
                     VGAQPGDYYPQPLRTEIDEVNRRVYDTLNNGVYKAGFATSQTAYEAAVNPLFETLDWL
                     EDRLSRSRYVCGDRLTEADIRLFTTLVRFDAVYHGHFKCNIRRIVDYKSLWAFTRDMF
                     QLPGIAETVNFTHIKRHYYMSHRRINPTGIVPVGPSLDFEAPADRGRDVDKLIVF"
     gene            1508..2446
                     /locus_tag="VGC82_06450"
     CDS             1508..2446
                     /locus_tag="VGC82_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0422949.1"
                     /translation="MEIRNLGRSGLRVSSIGLGCNNFGGRINEAATKLVIHKALDLGI
                     TLFDTADVYGERGGSEEVMGRILGDDRKRIVLATKFAAPMDDSEEMMGGSRRYIMAAV
                     EDSLRRLRTDWIDLYQMHRVDPRTPIEESLRALDDLIRQGKVRYIGCSNFPAWQMTEA
                     VWTARAAGLTGFVSCQDEYSLVHRAPEAELLPAARKLGFGLLPYFPLASGLLTGKYRR
                     NVNLPAGTRLAMTQRLADRYLSERNWEIAEKLGDFVEKRGRTMLELAFSWLLAQDPVA
                     SVIAGATRPEQLEQNVKAGGWVLTEDELAEVGRITS"
     gene            complement(2443..3639)
                     /locus_tag="VGC82_06455"
     CDS             complement(2443..3639)
                     /locus_tag="VGC82_06455"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0422950.1"
                     /translation="MTTPLAGITVLDFGQIFQGPYATMLMAKAGADVIKIEPPGGEPL
                     RRRVIASGSGDTTLPMAMLNANKRAVTLNLKADAGKHLLKQMVEKADVLLENFSPGTL
                     DNLGVGYEVLKEINPRLVYATGTGFGISGPDRDNLAMDFTIQAASGIMSITGDPDGPP
                     MKAGPTLVDFMGGIHLYAAVMTALLQRTVTGQGQLVEIAMQETVYSSLASSYDYHLRT
                     GKVPPRAGNRQAGLSSAPYNAFRTKDGWVAVHVVTEGHWQNLLRAMGRDDLLDDPRFS
                     TNTARTENMEATEALVTAWTLGLGKMEVVAAAKRYKIPVAAVRNAIEVMNDPHMHERG
                     MLQRIDHPSLGPIVVPNSPLRLHGSDRVDPVPSPSLGQHNAEVYGVWLGLGPDGVAAL
                     KQSGAI"
BASE COUNT          790 a         1213 c         1282 g          790 t
ORIGIN      
        1 cgcgaggccg ccgacgccag agacgatgcg gtgaaggccg gcgtgacgat caacggacta
       61 gcgatcatca acgaccaccc ggtgtcgtgg actttcgcgc acgtccagcc gccgggtggc
      121 ctggcgaact attaccggga gaacgtgacc ggcggtcccg gcaacttcgt gctggaggtg
      181 catgatttcg caagcttcgg cgaggcgatg acccggaagc tggtcgatga aatcgcggct
      241 gtgccgccgc tgtcgaacta tgccacgcgc agataacttc aggggatctg aatgggtttg
      301 ttgatcaatg gcgaatggca ggagcacgag cccgagacga cgaaggatgg ccacttcgag
      361 cgccgggaca ccgcatttcg caactggatc acgcccgatg ggcgccccgg accaaccggg
      421 caggatgggt tccgcgcggc agcgggacgc taccacctgt atgtttcgct cgcttgccca
      481 tgggcgcatc gcacgctgat catgcgcgcg ttgaagggat tgcagacgat catcccggta
      541 tcggtcaccc actggctgat ggccgagaat ggctggacct tcacgacggg gaacggcgtg
      601 atcccggatc cgctttacgg cagccggtac ctgtacgagc tttatgcgcg tgccgatgag
      661 acttactccg gccgctcgac ggtgccgatt ctgtgggacc agcacaccca gaccatcgtc
      721 aacaatgaat cggccgacat catccgaatg ctcggttcgg cgttcgatca cgtgggtgct
      781 cagccgggcg attattatcc gcagccattg cgcacggaaa tcgacgaggt caaccggcgc
      841 gtttacgata ccttgaacaa tggcgtgtac aaagccggct tcgccacttc gcagacagcg
      901 tacgaggccg cggtcaatcc gttgttcgaa acgctggatt ggctggagga ccggctatcc
      961 cggtcgcgct acgtctgcgg cgacagactg accgaggccg acatcaggct gttcaccacg
     1021 ctggttcgtt tcgatgcggt atatcacggt catttcaagt gcaacatcag gcggatcgtc
     1081 gattacaaga gcttgtgggc ctttacgcgc gacatgttcc agcttcccgg gatcgcggaa
     1141 acggtgaatt ttactcacat aaagaggcat tactacatga gtcaccgccg catcaatccg
     1201 actggaatcg tgcccgtcgg gccgtccctg gatttcgagg cgccggctga tcgtggccgc
     1261 gacgtggaca agctgatcgt cttttaaggc cgccaaacaa gatccggggc cgccgaacag
     1321 gatccggacg ggaaaggtcc gctgcgaggc ggtggtgcgg caatgcgaca tcccgctgga
     1381 taaacggctg tacgtcagcc ggcgtgggct acctggcaca cgggttacct gggacacggg
     1441 cttcccaact ggcaaatcgc tgtgttcgac tgcatgatcg gctgtcgcat ccggaaaggc
     1501 taccgtcatg gaaatccgaa atctgggccg atcgggcctg cgcgtttcat caatcggtct
     1561 cggctgcaac aactttggcg gcagaatcaa cgaggctgca acgaagctgg tgattcacaa
     1621 ggctttggac ctcgggatca cattgttcga caccgcggat gtttatggcg aacgcggcgg
     1681 atcggaagaa gtcatgggcc gcatcctggg cgacgaccgc aagcgcatcg tcctggcgac
     1741 aaaattcgcc gccccaatgg acgacagcga agagatgatg ggtggctcgc gccgctacat
     1801 catggccgcc gtcgaggaca gtctgcggcg acttcggacc gattggatcg acctgtacca
     1861 gatgcaccgg gtcgatcctc gcacgccgat cgaagaatcg ctgcgcgcat tggacgacct
     1921 gatccggcag gggaaggtgc ggtatatcgg gtgctcgaac ttcccggctt ggcagatgac
     1981 cgaggccgtg tggaccgcac gcgcagccgg cctgaccggg tttgtttcgt gtcaggacga
     2041 atattcgctg gtgcaccggg cgccggaagc ggaactgctg cccgcggcac gaaagttggg
     2101 cttcggattg ttgccctact tcccgctcgc gagtggattg ttgacgggga aataccgtcg
     2161 caacgtcaac ctgccggcgg gaacgcggct ggcaatgact cagcggctgg cagatcgtta
     2221 tttgagcgaa cgaaactggg agatcgccga aaagctcggc gatttcgtgg agaagcgggg
     2281 ccggacgatg ctggaactgg cgtttagttg gttgctggcg caagaccctg tggccagcgt
     2341 gatcgccggc gcgacccggc cagagcagtt ggaacagaat gtcaaggccg gaggctgggt
     2401 gctgactgag gacgaactag ccgaggtcgg ccgaataacc agctaaatcg cgcccgactg
     2461 cttcaacgcc gccacgccgt ccggtcccag accaagccac acgccgtaaa cctcggcgtt
     2521 gtgctgaccc aacgaagggc tgggcaccgg atcgacccga tcggatccgt gcaaccgcag
     2581 cggcgaattc ggcacgacga tcggacccag cgatgggtgg tcgatccgct gcaacatccc
     2641 gcgttcgtgc atgtgcggat cgttcatgac ctcgatcgcg ttgcgcaccg cggccaccgg
     2701 gatcttatag cgtttcgccg ccgctacgac ttccattttt cccaggccaa gggtccaggc
     2761 ggtgaccagt gcctcggtcg cctccatgtt ctcggtgcgc gcggtgttgg tgctgaagcg
     2821 cggatcgtcc agcagatcgt cccggcccat cgctcgcagc aggttctgcc aatgtccctc
     2881 ggtgacgaca tgaaccgcga cccagccgtc ttttgtccgg aacgcgttgt aaggggcact
     2941 ggacaatccg gcctgccggt tgccggcgcg aggcggtact ttgccggtcc gcaggtggta
     3001 gtcgtaactg gacgcaaggg acgaatacac tgtctcctgc atcgcaattt ccaccaactg
     3061 cccctgtccg gtcacggtcc gctgcaacag ggcggtcatg acggctgcat acaggtggat
     3121 accgcccatg aaatcgacca gcgtcgggcc ggccttcatc ggcgggccat ccggatcgcc
     3181 agtgatgctc atgatgccgg aggctgcctg gatggtgaaa tccatcgcca gattgtcgcg
     3241 gtcgggcccg ctgatcccga agccggtgcc ggtggcatag accagccttg gattgatttc
     3301 cttgagcacc tcatagccca cgcccagatt gtccagtgta ccgggcgaaa agttctccag
     3361 caacacatcg gctttctcca ccatctgctt cagcaggtgc ttgccggcat cggccttcag
     3421 gttcagcgtc acggcgcgct tgttggcgtt cagcatcgcc attggcaggg tggtatcgcc
     3481 gctcccgctg gcgatgaccc gtcggcgcaa cggttcgccg cctggcggtt cgatcttgat
     3541 gacgtccgct cccgccttcg ccatcagcat ggtggcatag gggccttgga aaatctggcc
     3601 aaaatccagc accgtgatgc ccgccagggg agtcgtcatc agagaacctc atgtgtcgaa
     3661 gtcgcagcgg cttcgactct aaagcatgat cgcgccgggc gaaacgtccg cggcgcgtgg
     3721 ctgatgcaat caaacgacgg acgccgagta ttcttgcacg agcagcaagg ctcgcggata
     3781 ccgttgtatt cccggctagt cccagcatgg ggatgccttt ttgtaggagg atcgaccacg
     3841 atttctttcc tgttcgccgc gggtttgata aagctctttc gggttgactg gaaaaggtgc
     3901 gagccggttc gccgcctgac gctatgtggc atggccgagc ttggtgcggc cacccacgac
     3961 ttcctttaca actacaaagg accctgtttt cagtcaccgt gaaatcgagc aagccgactg
     4021 gactttcttg atctgccgag ggcggacata ctggctgcgt gttgaacagg gcgac
//
LOCUS       DASWQN010000395         6106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2281417, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000395 DASWQN010000000
VERSION     DASWQN010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2281417"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VGC82_06460"
     CDS             complement(<1..457)
                     /locus_tag="VGC82_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0422951.1"
                     /translation="MPMPLDLTGHTALVTGGSRGIGAAIVRALAKAGAKVAINYRERA
                     ADADIVAAEVAATGRRAVTVAADVSNGEAVAEMVRMVQAQLGPIDILVNNAGIALIRG
                     IDDLTEADFDRTIAVNLKSAFLCTQAVLPHMRQQGWGRIVNISSGAARGA"
     gene            complement(480..1127)
                     /locus_tag="VGC82_06465"
     CDS             complement(480..1127)
                     /locus_tag="VGC82_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0422952.1"
                     /translation="MRDEVAWLYKQAAARGLIAGSSGNVSGRTPDGMMITPSGCDPET
                     ATATDLVRVAINGTVLGGGTPSSEWAMHAAVYRECDDAEFVVHTHADASTALACLGLA
                     LPAFHYMVVQFGGAEVRCAPYVTFGTPALAAHAAEAIRGRNACLLANHGMIVLAPTAA
                     QALSRAVLLETLCRQYLLALSAGVPRLLTEQEMQDAQERFKTYGQRPAAAGSDAT"
     gene            complement(1127..2179)
                     /locus_tag="VGC82_06470"
     CDS             complement(1127..2179)
                     /locus_tag="VGC82_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0422953.1"
                     /translation="MQGVVFTGERGLELMTFPDPTPGPGEVVIEMKASGMCGSDLHQY
                     RRPKSQPRNATGLPPNPNPTIAGHEPCGVVAAIGPDVLPAEAKIGQRVMVHHYQGCTQ
                     CGHCRSGWQQLCQEVPVKVYGSNAHGGHAKYLQVPANTLVPLRDELSFSSGAAIACGS
                     GTAYGALRRMNISGRETIAIFGQGPVGLAGTQFAKAMGARVIALDINPHRLERAKEFG
                     ADEVVNPGSNDPVAAIKELTSGKYADLTLDTSSNPEARLNAIRSTKVWGTMCFVGEGG
                     DVHIDVSPHLLRRQLTLVASWTFSNIIQAECADFCVERKIDVDKLFTHRWTLDQADEA
                     YKLFDKQSDGKGVFLI"
     gene            complement(2252..4000)
                     /locus_tag="VGC82_06475"
     CDS             complement(2252..4000)
                     /locus_tag="VGC82_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0422954.1"
                     /translation="MKTKRSDRTFMLLRRLWCEHLVHYKPRLLLVVALTLVMAGTTAL
                     YPVVINHAFDMFTDHDRRILYQIPLLVVVVTGLKAIAQYFQNVQVQQIVLLVIRELQG
                     RMFAHLTRADLARMEREAPAQLAAHFTTDAATIREALTRAVNGVADVITVIGLVGSMI
                     YLNWVLSLIAAAMYPVAILPIERIGRRVRRASGGMQERVGETAALLNESFAQARTVRA
                     YRLEASETRRAEAAFAQLYKSLLRMAKSRSRVDPVLEVLGGVAVAAVIGFEGWRAAVS
                     NHALGDFTGFVAALLIAARPLRALGSLNAALQEGLAGMVRVFTIIDEQPTIKDAPDAK
                     PLPAGRGHVTFDNVRFVYPDGREGLRGLSFEALPGTTVALVGPSGGGKSTALALIPRL
                     QDPTEGALRIDGADVRTLTLASLRDSIAYVGQDALLFDDTVAANIAMGRPGASNTEIE
                     AAAEAAAASFVAALPLGYETVVGPGGQRLSGGQRQRISLARALLRDPRILLLDEATSA
                     LDTESEAAVQMAVAALRKGRTTIVIAHRLSTVRDADLVVAMAEGRAQESGTHAALMDQ
                     DGLYARLVRSQSLLRA"
     gene            4085..5029
                     /locus_tag="VGC82_06480"
     CDS             4085..5029
                     /locus_tag="VGC82_06480"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY0422955.1"
                     /translation="MANRINRTIELLEQGQAIYYDGQHSGHVLTYEQGRRDAGTWADY
                     MNVGMEHGAFDMTGLAEYMRGLTDGGPTRSGHRTPAVIVEAPLNGTDEANVRFNAWQF
                     RQILGRGVHGILLCQAETADAVRAFVEACRYPHQLHAVDPGLPSPEARLAGAVRSRAT
                     DGSLGIGTRGRGSENTAAPVWGLSGDEYMDRCDPWPLNPRGELLLGVKLESPEGIANA
                     DAICAVPGLGFAEMGPGDLSLSLGSVKLLRDPYPPVMQAARDRVFAACRRHGIAFLEG
                     CNPANISQRLDEGVRVISGHSEEMARIGRAHQKRQMPV"
     gene            5198..5362
                     /locus_tag="VGC82_06485"
     CDS             5198..5362
                     /locus_tag="VGC82_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422956.1"
                     /translation="MNILIRAALAAISLATITPVANAAMWGTPQSTYHSGPYDNTGHG
                     GNTGGGGGGG"
     gene            5549..>6106
                     /locus_tag="VGC82_06490"
     CDS             5549..>6106
                     /locus_tag="VGC82_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0422957.1"
                     /translation="MGVTTTLSAFTAAIRLDMLPPEVVNRARFLVLDLVGNIVRARHD
                     AESTASFLAATRAMGLASGNSGVFGDAARYTPAGAAFLNGALAHSLDFDDTHAAGSLH
                     PGAPVIPAALAAGEMVGASGADVLAAIIAGYEMTCRVALALPAGEHYDRGFHPTATCG
                     AFGAAAAAARVFGLDANGVAGALGTV"
BASE COUNT         1091 a         1945 c         1969 g         1101 t
ORIGIN      
        1 cagcgccccg cgcagcgccg gaggagatat ttacgatgcg cccccagccc tgctgccgca
       61 tatgtggcag gactgcctga gtgcatagaa aagcagattt gagattaacc gcgatcgtcc
      121 gatcgaaatc ggcctcagtc agatcgtcga taccgcgaat aagcgcgatg ccggcgttgt
      181 tgaccaggat atcgatgggg ccgagttgcg cttgaaccat ccgaaccatt tcggccacgg
      241 cctcgccgtt ggatacatcc gcggccacgg tgacagcacg gcggccggtc gcggcaacct
      301 cggcggccac gatgtcagcg tcggcggcgc gctctcggta gttgatggca accttcgcgc
      361 cggctttcgc caatgcgcgc acgatggcgg cgccgatccc acgggagccg ccagtgacga
      421 gtgctgtatg tccggtcagg tccaatggca tcggcatcga tcaagctttc tgaatatcgt
      481 tacgtcgcgt cagaccccgc cgctgccggt cgctgtccgt acgtcttgaa gcgttcctgt
      541 gcgtcctgca tctcctgctc ggtcagcaat cggggcacac cggcagacaa tgccaataga
      601 tactgccggc acagggtctc cagcaggacg gcgcgggaca acgcctgcgc tgccgttggg
      661 gcaagaacga tcatgccgtg attagcgaga agacaggcat tccggccgcg aatcgcctcc
      721 gcggcatggg ccgccaaagc cggggtcccg aacgtcacgt acggcgcgca ccgcacctcg
      781 gccccaccga actgtacaac catgtagtga aaagccggca aggccaatcc caggcaggcc
      841 agcgcggtgg aggcatctgc atgggtatgt acaacgaact cggcatcgtc gcattcccgg
      901 taaaccgccg cgtgcatcgc ccattcgctg gagggcgtgc caccccccag aaccgtccca
      961 tttatcgcga cccgcaccag gtccgtcgcc gttgccgttt ccgggtcgca gccggacggg
     1021 gtgatcatca tcccgtccgg cgtgcggcca ctgacattcc cggaactccc ggcgatcagt
     1081 ccccgggccg ccgcttgctt gtaaagccag gcgacctcgt cacgcatcag atcaggaaca
     1141 cgcccttacc gtccgactgc ttgtcgaaca gcttgtaggc ctcatccgct tgatccagcg
     1201 tccagcggtg cgtgaacagc ttgtccacgt cgattttccg ctcgacgcag aaatccgcgc
     1261 attccgcctg gatgatgttc gaaaacgtcc aggacgctac aagggtcagc tgacgacgca
     1321 gcagatgcgg actgacgtcg atatgcacgt cgccgccttc gccgacaaag cacatcgtgc
     1381 cccaaacctt ggtgctgcgg atagcgttca accgagcctc gggattggat gaggtatcga
     1441 gggtcaggtc ggcgtattta ccgctggtca attcctttat cgcggcgaca ggatcgtttg
     1501 agccggggtt gacgacttcg tcagcgccga attccttcgc acgctccagc cgatgcggat
     1561 tgatgtcgag cgcgatcacc cgtgccccca tggctttggc gaactgcgtg ccggccagcc
     1621 cgaccgggcc ttgcccgaag atcgcgatgg tctcgcgccc cgagatgttc atccgccgca
     1681 gcgcgccata cgcggtgccc gacccgcagg cgattgctgc gcccgatgag aaagaaagct
     1741 cgtcgcgcag cggcacaagc gtattcgccg gcacctgcag gtatttggcg tgtccgccgt
     1801 gcgcgttgct gccatacacc ttgactggca cttcctggca aagttgctgc cagcctgaac
     1861 ggcagtgacc gcactgcgta cagccctggt agtgatgcac cattacccgc tgaccgatct
     1921 ttgcctcagc cggcagcacg tcgggcccga tggcggcgac gacgccgcat ggctcgtggc
     1981 cggcgatggt cgggttcggg ttaggcggca gcccggtcgc attgcgcggc tggctctttg
     2041 gccggcggta ctgatgcagg tcggacccgc acatgccgga agccttcatc tcgatgacga
     2101 cttcgcccgg gcctggcgtg gggtccggaa acgtcatcag ttccaacccg cgctcgccgg
     2161 taaacacaac accctgcatg gtttcctccg tctttctgta tggccggcag tctattccca
     2221 ttttggccgc agggaaccag ctccgccttc cttatgctct cagcaaactc tggcttcgca
     2281 ccagcctggc ataaagtccg tcctggtcca tcagcgctgc gtgggtgccg ctttcctgcg
     2341 cgcgcccttc cgccatcgcc acgaccagat cggcatcgcg cacggtcgaa agccggtgtg
     2401 cgatcacgat ggtggtgcgc cccttgcgca aggcggcgac cgccatctgc accgccgcct
     2461 cgctttcggt gtccaatgcg ctggtggctt cgtccaacag cagaatcctc gggtcgcgca
     2521 gcagcgcccg agccagcgag atgcgctggc gctgaccgcc tgaaagccgc tgcccgcctg
     2581 ggccgacgac ggtctcataa cccagcggca gggcggcgac gaaactagcg gcggcggcct
     2641 cggcggcggc ctcgatctcg gtgttcgagg cgcccggccg tcccatggca atgttcgcgg
     2701 ccaccgtgtc gtcgaacagc agcgcatcct ggccgacata ggcgatactg tcgcgcaggc
     2761 tggccaaggt gagggtccgc acgtcggcgc catcaatccg caaagcgcct tcggtcggat
     2821 cctgcaagcg ggggatcagg gcgagtgcgg ttgacttacc tccacccgac ggacctacca
     2881 gtgccacggt cgtacccgga agcgcctcga aactcaaacc gcgcaagcct tcccgtccgt
     2941 ccggatagac gaaacgcacg ttatcgaacg ttacatgacc tcgcccggcg ggcagcggct
     3001 tggcatcggg tgcgtccttg atcgtcggct gctcgtcgat aatggtgaag acgcggacca
     3061 tgccggccaa accctcctgc agggccgcgt tgagcgagcc gagggcgcgc aggggcctcg
     3121 cggcgatcag cagggcagcg acgaaaccgg tgaaatctcc gagcgcgtgg ttgctaacgg
     3181 cggcgcgcca gccctcaaag ccgatgacgg cggcaaccgc gacaccgccg agaacctcca
     3241 gaacaggatc gacgcgggag cggcttttcg ccatccgcag cagggatttg tagagctggg
     3301 cgaaagcggc ctccgcgcgg cgggtttcgc tggcttccag gcggtacgcc cggacggttc
     3361 tggcctgggc aaaactctcg ttcagcaaag cggcagtttc tccgacacgt tcctgcatgc
     3421 cgccggaggc gcggcggacg cggcggccaa tccgctcgat cggcagaatg gctaccggat
     3481 acattgcggc ggcaatcagc gagaggaccc aattgaggta gatcatgctg ccgaccaagc
     3541 cgatgaccgt gatcacgtcc gcaacgccgt tgaccgctcg ggtcagcgcc tcgcggatcg
     3601 tcgctgcatc ggtggtgaaa tgggccgcga gctgcgccgg cgcctcgcgc tccatccgtg
     3661 ccaggtcggc tcgggtcaga tgggcgaaca tgcggccctg gagttcgcgg ataaccagca
     3721 ggacgatctg ctggacctgg acattctgga aatattgcgc gatcgccttc aggccggtga
     3781 ccaccacgac cagaagcggg atctgataaa ggatgcgccg atcgtggtcg gtgaacatgt
     3841 cgaatgcgtg gttgatgacg accgggtaca gcgcggtcgt gccggccatc accagggtca
     3901 gggcgacgac cagcagtagc cttggcttgt agtggaccag gtgctcgcac cacaggcggc
     3961 gcagcagcat aaaggtgcgg tcggagcgct tcgtcttcat ggccggccat gtaccagcag
     4021 atgcgctatt tcgctatgcg ggttacgagg tgcagactgg cccgattagt gcgggaggac
     4081 gaccatggcg aaccggatca accgaaccat cgaactgctg gagcagggcc aggcaattta
     4141 ttacgacggc caacacagcg gccatgtact tacatatgag caaggcagac gcgatgccgg
     4201 gacctgggcc gactacatga atgtcggcat ggagcatggc gcattcgata tgacgggcct
     4261 tgccgagtac atgcgtggtc tgaccgacgg cggacccacc cgttcgggcc atcgcacgcc
     4321 ggcggtgatc gtcgaggccc cgttgaacgg caccgacgag gcgaatgtcc ggttcaatgc
     4381 gtggcagttc aggcagatcc tggggcgagg ggtgcatggc atcctgctgt gccaggccga
     4441 aaccgccgat gcagttcgtg ccttcgtgga ggcttgccgc tatccgcacc agctgcacgc
     4501 ggtcgatccc gggttgcctt cgccggaagc tcgtttggca ggcgcggtcc ggtcgcgggc
     4561 gacggacggt tctttgggca ttggcacgcg cgggcgtggg tcggagaaca cggccgctcc
     4621 ggtttggggt ttgtccggcg atgagtacat ggatcgctgc gacccctggc cgctgaaccc
     4681 gcgaggcgaa ttgctgctgg gcgtcaagct ggaaagtccc gaaggtatcg ccaatgccga
     4741 tgcgatctgc gccgtgccgg gactgggctt cgccgagatg gggccaggag acctgagttt
     4801 atcgttagga tcggtaaagc tgctgcgtga cccgtatccg ccggtgatgc aggccgctcg
     4861 ggacagggtt ttcgcggcgt gccggcggca cgggatcgcg ttcctggagg ggtgcaaccc
     4921 agcgaatatt tcgcagcggc tggatgaggg ggttcgggtg atctccgggc atagcgagga
     4981 aatggcgcgg atcgggcggg cgcaccagaa gcggcaaatg ccggtgtagc gtcgaggccg
     5041 ccgcatctgg cgattacacg aagtccgaat cccagccacc tcatcggccg gcgcataaat
     5101 ttaatgtctt agaaccggat tattgttcat cacgagttgg tccatctacc acccaacaaa
     5161 gccggacatc gcgcccgacg ggagatggag aattccgatg aacatactca ttcgtgcagc
     5221 gcttgccgct atcagcctgg caactatcac tccagtggcc aatgccgcca tgtggggcac
     5281 accgcaaagc acctatcatt ctgggcctta tgacaatact ggccatggtg gaaacacggg
     5341 cggcggcggc ggcggcggct aagaactccc tggcatgttg cacggaggcc gggtcatccc
     5401 ggccttcgcc gtgcgtgaag catgggcgat gagatgattg gttgagcagg cgtcgccact
     5461 aggccatggc agatcagcgc ataagaccac cttggcctcg gccgcgccct gtcgcagaat
     5521 acatccatta gccaggagga gcctgaccgt gggcgttacc accacccttt ccgccttcac
     5581 cgccgcgatc cggcttgaca tgctgccgcc tgaagtagtg aaccgcgcgc gcttcctggt
     5641 cctcgatctt gtcggcaata tcgtgcgtgc gcggcatgac gctgaaagta ccgcctcgtt
     5701 ccttgccgcg actcgtgcaa tgggactggc ttccggcaac tcgggcgtgt tcggcgatgc
     5761 ggcgcgatac acccctgccg gtgccgcttt cctgaacggg gcactggctc attcgctgga
     5821 cttcgacgac acccatgccg ccggatcgct gcacccggga gcgcccgtca tccctgccgc
     5881 gctagctgcg ggcgaaatgg tcggagcctc gggcgcggac gtgctggcag cgatcatcgc
     5941 cgggtatgag atgacgtgcc gggtggcgct ggcgttgcca gccggggagc attacgatcg
     6001 aggctttcac cctaccgcta cgtgcggtgc ttttggggct gccgctgccg cggcgcgggt
     6061 ttttggcctt gatgccaacg gtgtcgccgg ggcgcttggg accgta
//
LOCUS       DASWQN010000396         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1357799, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000396 DASWQN010000000
VERSION     DASWQN010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357799"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VGC82_06495"
     CDS             complement(<1..200)
                     /locus_tag="VGC82_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0422958.1"
                     /translation="MSTKPGLRSKKRLVCDDGWRLPEVLWAKMEPLLPPRPRHPLGCH
                     NPRVPDRAAMDAIFFVLRTGCQ"
     gene            283..1182
                     /locus_tag="VGC82_06500"
     CDS             283..1182
                     /locus_tag="VGC82_06500"
                     /inference="COORDINATES: protein motif:HMM:NF018400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1186 domain-containing protein"
                     /protein_id="HEY0422959.1"
                     /translation="MTEPLSTDAILDALSATNGVLPRAAMQQAIARWPEVGPVLLTRL
                     NAAIDSTDISQREGNLLCMAIYLMAQVQDHSAYRPLCTLLMTGDRGYDLIGDGVTEDL
                     KFILAWLYDGDSAPLRTLIEATGANEFTRDAALNAFAWLTATGRIDREEAVAYFRDLH
                     ASLQPQAACYVWVSWLLAIANLGLEELSPLVEDVFQRGWIDDTILSVEDFRNDLAAAK
                     QAGGLIAGFPPEERYDGRLDDVAHTMSNWQMFQPQKPTRQTSLPAPDGGTVRNPYRGV
                     GRNDPCPCGSGKKFKKCCLELVR"
     gene            complement(1225..>1959)
                     /locus_tag="VGC82_06505"
     CDS             complement(1225..>1959)
                     /locus_tag="VGC82_06505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0422960.1"
                     /translation="RISGVVTERGTIACDAAVLAGGAWSRLFCGNMGVDFPQLKVLGS
                     VMRTEPLNGPPDVAVAGPDFAFRKRADGGYTVARRNKYEAHIVPDSFRLLPRFFSTMI
                     ATRREYRIRMFGRFREEARIPRRWALDQASPFEATRVLDPAANPAIVEESRRNLVAAF
                     PAFAGARIAESWGGLIDSTPDAVPVIAPVQAVPGFFLMSGFSGHGFGIGPGAGRLMAE
                     LITGATPVVDSAPFRFERFGSVVRTA"
BASE COUNT          376 a          649 c          618 g          316 t
ORIGIN      
        1 cactggcagc cggtgcgcag cacgaagaag atcgcatcca ttgctgcacg gtcgggcaca
       61 cgcgggttgt ggcagcccaa cggatgcctc ggtcgcggcg gcagcaacgg ctccatcttc
      121 gcccacaaga cctctggcag acgccagccg tcatcgcaca ccaggcgctt tttcgatcga
      181 agtccaggct ttgttgacat gcatgaaatc tggcaccgga aatgccaggt ttcacctact
      241 gggatacgct ctaagcagaa acgcaccaag gccgatccgc cgatgaccga accgctcagc
      301 accgacgcta ttctggacgc tttgtcagcc accaatggag ttctgccgcg cgctgcgatg
      361 cagcaggcga ttgcgcgctg gccggaggtc ggtcctgtgc tgctgacgcg cctgaatgcc
      421 gcgatcgaca gcaccgacat ctcccaacgc gagggcaacc tcctgtgcat ggccatctac
      481 ctgatggcgc aggttcagga ccacagcgcc tatcgcccgc tgtgcaccct gctcatgacc
      541 ggcgaccgtg gctacgacct gatcggtgac ggtgtcacgg aggacctgaa gttcattctg
      601 gcctggctct acgacggcga ttccgcgccg ctacggacgc tgattgaagc aaccggggcg
      661 aatgaattta cccgcgatgc ggccctgaac gcattcgcct ggctgaccgc gaccggccga
      721 atcgaccgcg aggaagccgt agcgtatttc cgcgaccttc acgcaagcct gcaaccgcaa
      781 gccgcgtgct acgtctgggt cagttggctg ctggcgatcg ccaaccttgg cctcgaagaa
      841 ctcagtccgt tggtggagga tgttttccaa cgcgggtgga tcgacgacac gatcctcagt
      901 gtcgaggatt ttcgcaacga cctggccgcg gcgaagcagg ctggcggcct gatagccgga
      961 tttccgcctg aggaacgcta cgacggacga ctggacgacg tggcgcacac catgtcgaac
     1021 tggcagatgt tccagccgca gaagccgacc cgacagacgt cgctcccggc accggatggc
     1081 ggcaccgttc gcaacccata tcgcggcgtc gggcgcaatg atccgtgccc gtgcggcagc
     1141 ggcaagaaat tcaagaaatg ctgcctcgaa ctggtccggt agcccggact gccggaagat
     1201 cctgcgggcc ccggcgtccc gctgtcaggc tgttcggacg accgaaccga agcgctcgaa
     1261 gcgaaacggc gcagagtcga cgacgggcgt ggcgccagtg atcagttccg ccatcagccg
     1321 ccccgcgccg ggaccgatgc cgaagccatg tccggagaac ccgctcatga ggaaaaatcc
     1381 tggcaccgcc tgcaccggcg cgatcaccgg caccgcatcg ggtgtgctgt cgatcagccc
     1441 gccccagctt tcggcgatcc gcgcaccggc gaacgccgga aacgcggcga ccagattgcg
     1501 ccggctttcc tcgacaatgg cgggattggc tgccgggtcg agcacgcggg tcgcctcgaa
     1561 cgggctggcc tgatccagcg cccaccgccg cggaatgcgg gcttcctcgc gaaaccgccc
     1621 aaacatccgg atgcggtact cgcgacgggt cgcgatcatg gtggagaaga accgcggcag
     1681 cagccggaag ctgtccggga cgatgtgggc ctcgtattta ttgcgtcggg caacggtgta
     1741 gccaccgtcg gcacgtttgc ggaaggcgaa gtcaggcccg gctaccgcca cgtcgggcgg
     1801 gccgtttagc ggttcggtgc gcatgacgga gcccagcact ttcaactgtg gaaaatccac
     1861 gcccatattg ccgcagaaca gccgcgacca ggcgccgcct gccagcaccg cagcatcgca
     1921 ggcgatggtg ccgcgttcgg tgaccacacc gctgatacg
//
LOCUS       DASWQN010000397         3146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1753819, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000397 DASWQN010000000
VERSION     DASWQN010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753819"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VGC82_06510"
     CDS             <1..583
                     /locus_tag="VGC82_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEY0422961.1"
                     /translation="DGFTHMFENMLDHPNITLMLGVPYQDVQDDINADHLVYTGPVDE
                     YFGFRYGPLPYRSLRFDHRTLDMETFQPVAVVNYPSPDVPYTRMTEFKHLTGQAHRKT
                     SICYEFPTADGDPYYPVPRPENAALYARYQALADATPGVTFVGRLATYRYYNMDQVVG
                     QALSAFARLTGGSQHADDILRPSNAYAKATAAS"
     gene            580..1914
                     /locus_tag="VGC82_06515"
     CDS             580..1914
                     /locus_tag="VGC82_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY0422962.1"
                     /translation="MIEAAVGTPSAVAGRPKVEIWGGVECSVVRIGNEWRDQVHETGH
                     HARDDDLDRIVDLGITTLRYPIVWERVAPRAPGEYDWQWHDRRMDRLRALGLQPIVGL
                     LHHGSGPHYTNLLDPDFPAKLAAYASAVAARYPWVGAWTPVNEPVTTARFSGLYGHWF
                     PHERGLEPFCRMVVNQCLGVLLAMRAIRKVIPGAKLVQTEDLGRVFATPQLMYQADHE
                     NLRRWLSLDLLCGELGPDHPWWPMLLSLGVPEASLKMLYHNDHPPDIIGINHYLTSDR
                     YLDSDKGRYPEVFAGGNGRERYADVEAVRIVPPPGPLGPEARLREAWERYKRPLAVTE
                     VHHGAAELVECVRWLGETWHAAERLHAEEVDIRAVTIWSLFGAVDWRSLLLQRAGAYE
                     PGAFDIRHDPPRATALAEAVVSLMRHRRIVDPAANGPGWWRRPERVYALPPR"
     gene            1939..2940
                     /locus_tag="VGC82_06520"
     CDS             1939..2940
                     /locus_tag="VGC82_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0422963.1"
                     /translation="MSRFAPPGPLGFGGAPLGNMFAAVDEATAQATMVAAWDSGIRYF
                     DTAPHYGAGLSEHRFGTMLRQHPRDSFVLSTKVGRLLTPDSEVLAGPPPFDAGLPFRA
                     SYDYSADGTMRSIEDSLQRMGLARVDVVYVHDIAEDAHGPGWREPFAAAMKGAGQALI
                     RLREQGVIRAWGLGVNRIEACQAALDQSDPDVFLLAGRYSLLDHSALRTLFPSCVARG
                     VHVIVGGPFNSGLLAGGAHFDYVPASAELLAKRARLQEVCDSYEVDIRASALQFCAAH
                     PAVCAVIPGAKSPDKVVQNAYLMRQPIPTAFWAALKEAKMLPIDAPTPSNEDSASAA"
BASE COUNT          538 a          983 c         1028 g          597 t
ORIGIN      
        1 agacggcttc acccacatgt tcgagaatat gctggaccat ccgaacatca cgctgatgct
       61 cggtgtgcca tatcaagacg tgcaggacga catcaatgcc gatcatctgg tatataccgg
      121 accggtggac gagtatttcg gtttccgcta cgggccgttg ccttatcgtt cgttgcgatt
      181 cgatcacagg acgctggaca tggagacgtt ccagcccgtc gcagtcgtca actacccctc
      241 ccccgacgtg ccctatacgc gcatgaccga attcaagcac ctgaccgggc aggcgcacag
      301 aaagaccagc atctgctacg agtttccgac agccgatggg gatccgtact atcctgtgcc
      361 acggccggag aacgcagcac tctacgcccg ctatcaggcc ttggcggatg caacaccagg
      421 agtgacgttc gtcggccgcc tggcgaccta tcgctactac aatatggatc aggtggttgg
      481 ccaggcgctg tccgcgttcg ctcgactgac tggcgggtcg cagcatgccg acgacatcct
      541 ccgaccgtcg aacgcgtacg ccaaagcaac ggcggcttca tgatcgaagc ggctgtcggg
      601 acgccctcgg ccgtggcggg caggccgaag gtcgagattt ggggtggcgt cgagtgcagc
      661 gtcgtcagga ttggcaacga gtggcgcgac caggtgcacg aaaccggtca tcacgcacga
      721 gacgacgacc tggaccgcat cgtggatctt ggcattacga cattgcgcta cccgatcgtc
      781 tgggagcggg tcgcgccgcg ggcgcctggg gagtatgact ggcagtggca tgacaggcgg
      841 atggaccggc tgcgggcctt gggcctgcaa cctatcgtgg gtctgctgca tcatggctcc
      901 gggccgcatt acaccaatct tctcgacccc gactttcccg ccaagctggc cgcctatgcc
      961 tcggccgtcg ccgcccggta cccctgggta ggggcctgga cgccggtgaa cgagccagtg
     1021 acgacagccc ggttctcggg tctttacgga cactggtttc cgcacgaacg cggcctcgag
     1081 ccgttctgtc gcatggtcgt caaccagtgc ctgggggtgc tgctggcgat gcgagccatc
     1141 cgcaaggtga tccccggcgc gaagctggtg cagacggagg atctggggcg tgtgttcgcc
     1201 accccacagc tcatgtatca agccgatcac gaaaatcttc ggcggtggct atcgctcgat
     1261 cttctttgcg gcgagctcgg cccggaccat ccgtggtggc cgatgctcct ttcacttggc
     1321 gtgccggaag cgtcgttgaa gatgctttat cacaatgacc atccgcccga cattatcggc
     1381 atcaaccatt accttaccag cgatcgctac ctggacagcg acaaaggccg ctatccggag
     1441 gtgtttgccg gcggcaatgg tcgcgaacga tacgccgatg tcgaggcggt tcgcatcgtc
     1501 ccaccacccg gcccgctggg tcccgaggcg cggcttcggg aggcatggga gcgctacaag
     1561 aggcccctgg cggtgacgga ggtccatcac ggcgcggcgg aactggtcga atgcgtgcgc
     1621 tggctgggcg aaacctggca cgcagccgaa cggcttcacg ccgaggaggt ggacatccgg
     1681 gcggtaacga tctggagcct gttcggcgcc gttgattggc gttcgttgct gcttcagcgg
     1741 gccggggcct atgaacctgg cgcattcgat atccgccacg atccgccgcg tgcaaccgcc
     1801 ttggccgaag ccgtggtttc cctcatgcgg catcggcgta tcgtcgatcc tgcggcgaat
     1861 ggcccgggat ggtggcgccg tccggagcgt gtctatgcgc ttccaccacg ctgaatcttg
     1921 attttcggag ggacgtcgat gtcgcgcttc gcaccacccg gaccattggg ctttggcggc
     1981 gcaccgttgg gcaacatgtt cgccgcggtc gatgaggcga ccgcccaggc aacaatggtt
     2041 gcagcctggg acagcggcat ccgctatttc gacacggcac cgcattacgg ggccgggctg
     2101 tcggaacatc ggttcggcac gatgctgcgt cagcacccgc gagattcatt tgtattgtcc
     2161 acaaaggtcg gccggctgtt gacgccggat tccgaggtgc tggcgggacc gccgccgttc
     2221 gacgccggac ttccgtttcg ggcgagctac gactattccg cggacggcac catgcggtcg
     2281 atcgaggaca gcctgcagcg catgggtctg gcgcgcgtgg acgtcgtcta tgtccatgac
     2341 atcgctgagg atgctcacgg tcccggctgg cgcgaaccgt tcgccgcggc gatgaaaggc
     2401 gcaggacagg cactgatccg gctgcgcgag caaggggtga tccgtgcctg gggtctgggg
     2461 gtgaaccgga tcgaggcatg ccaagcggcc ctggatcaat cggaccctga cgtcttcttg
     2521 ctcgccgggc ggtactcgct gctcgatcat tccgcgctgc gcacgctgtt cccgtcctgc
     2581 gtggcgcgcg gggtccatgt gatcgtgggc ggcccgttca actcgggcct gctcgccgga
     2641 ggtgcgcatt tcgattatgt gccggcctcg gcggaactgc tggcgaagcg ggcccgcctg
     2701 caggaagttt gcgactcgta cgaagtggac atccgagcgt cggcgctgca gttttgtgcc
     2761 gcccaccctg cggtctgcgc cgttatcccc ggcgccaagt cgccagacaa ggtggtgcag
     2821 aacgcgtacc tcatgcgcca gccgattcct acggcatttt gggcggcgct gaaggaggca
     2881 aaaatgctgc cgatagatgc accgacgccg tccaacgagg attcggcttc ggcagcatag
     2941 gttcaggcag gcggcggtct gacctctgct ggatgcagcc atccgttcac gggccgatgc
     3001 gccggcagtt cgggctccag gcactgcatt cgcgcgccaa agccgcaact atcgcttgcg
     3061 tttgaaaggc agaacgaatg ttgcatgaac cgcccgatgc tcgccacgct tatgcactgg
     3121 atcggcggcg cctgctggca gcggcg
//
LOCUS       DASWQN010000398         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1886031, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000398 DASWQN010000000
VERSION     DASWQN010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1886031"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VGC82_06525"
     CDS             complement(<1..828)
                     /locus_tag="VGC82_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY0422964.1"
                     /translation="MFRTLLIANRGEIACRIARTARRMAIETIAVYSEADAGALHVQA
                     ADRAYPIGPAPARESYLNISRIIETARAAGAAAIHPGYGFLSENPAFAEACAAAGIVF
                     VGPPPSAMRTMGSKSAAKALMEKSAVPLLPGYHGDRQDAAFLEHQAVGIGFPVVIKAV
                     SGGGGRGMRVVGSSGEFAAALASARQEAASAFGDDRVLIERYLQRPRHIEIQVFGDRQ
                     GNVVHLFERDCSVQRRHQKVLEEAPAPGLDSRTRSEMGAAAVAAARAIRYAGAGTVEF
                     "
     gene            complement(834..2426)
                     /locus_tag="VGC82_06530"
     CDS             complement(834..2426)
                     /locus_tag="VGC82_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY0422965.1"
                     /translation="MTIIRSDISTRSAEFSANAESMRGLVADLRETVAEISLGGGTAA
                     RDRHVARGKLLPRDRVAALIDSGSPFLEFSQLAAFGLYGGDVPAAGIVTGVGRVSGRE
                     CVIVANDATVKGGTYFPMTVKKHLRAQEIARKNNLPCVYLVDSGGAFLPMQDEIFPDR
                     EHFGRIFFNQANMSAEGIPQIAVVMGSCTAGGAYVPAMSDESIIVRKQGTIFLGGPPL
                     VQAATGEIVSAEDLGGADVHARTSGVVDHYARNDHHALSICRRIVAGLNRTKTVGLSL
                     RPLEAPKYDPAELHGVVPADLRAPYDAREVIARIVDASDFDEFKKLYGTTLICGFAHL
                     YGYPVGIVANNGILFSESSLKGAHFIELCSQRNIPLIFLQNITGFMVGRKYESGGIAK
                     DGAKLVTAVSTTAVPKITLIVGGSYGAGNYGMCGRAYDPRFLFMWPNARISVMGGEQA
                     AGVLATVRSGSAGWTKEQEEAFKAPIKEQYDRQGHPYYASARLWDDGIIDPADSRRVL
                     GLSLSAALNKPMQDTRFGVFRM"
BASE COUNT          454 a          820 c          760 g          412 t
ORIGIN      
        1 gaattcgacg gtgccagcgc cggcgtaacg gatcgcgcgc gccgctgcaa cggcggccgc
       61 gcccatctcg cttcgcgtcc tggaatccag cccgggcgcc ggagcttcct cgagcacttt
      121 ctggtggcgg cgctgcactg agcagtcgcg ctcgaaaagg tgcaccacgt tgccctggcg
      181 gtcgccgaac acctggatct cgatatgccg gggccgctgc agatagcgct cgatcagcac
      241 gcggtcgtcg ccgaacgcgg acgctgcctc ctgccgggcg gaggccaggg ctgcggcgaa
      301 ttccccggac gagcccacaa cccgcatgcc gcgtccgccg ccgccggaga ccgccttgat
      361 caccacagga aagccaatcc cgaccgcctg gtgctccaga aacgccgcat cctggcgatc
      421 gccatggtag cccggcagca acggcaccgc ggatttttcc atcaatgctt tcgccgccga
      481 cttcgatccc atcgtacgca ttgccgaggg cggcggtccg acaaagacga ttccagcggc
      541 ggcacacgcc tcggcgaatg cgggattctc cgacagaaag ccataaccgg ggtggatcgc
      601 cgccgcgccg gccgcgcgtg cggtttcaat gattcgggaa atgttcagat agctttcgcg
      661 cgccggggct ggtccgatag gataggcccg atccgccgcc tgcacatgca gcgccccggc
      721 atcggcttcg gagtaaaccg cgatcgtttc aatggccatg cggcgcgcgg tgcgggcgat
      781 gcggcaggcg atctcgcccc ggttggcgat cagcagggtg cggaacatcc cggtcacatc
      841 cggaacacgc cgaagcgcgt atcctgcatg ggcttgttca aggccgccga caatgacagg
      901 cccagcaccc ggcggctgtc ggcggggtcg atgatgccgt cgtcccacag gcgggcgctg
      961 gcgtaatagg gatggccctg gcgatcgtac tgttccttga tgggtgcctt gaaggcttcc
     1021 tcctgttcct tggtccaacc ggcggagccg cttcggacgg tggccagcac accggccgcc
     1081 tgttccccac ccatcaccga aatccgggcg ttgggccaca tgaacaggaa cctgggatca
     1141 taggcacgcc cgcacatgcc gtaattcccg gcaccgtagc tgccaccgac gatcaaggtg
     1201 attttcggca cggcggtggt ggaaaccgcg gtgaccagct tggcgccatc cttggcgatg
     1261 cctccggatt cgtatttgcg gcccaccatg aacccggtga tgttctgcag gaagatcagc
     1321 ggaatgttcc gctggctgca aagttcgatg aagtgcgcgc ctttcaacga ggattccgag
     1381 aacaggatgc cgttattcgc gacgatcccg accggatagc cgtacagatg cgcaaagccg
     1441 caaatcagtg tcgtgccgta cagcttcttg aactcgtcga agtcgctggc atcgacgata
     1501 cgggcgatga cttcccgagc gtcgtatggc gcacgcaagt cggcgggaac cacgccgtgc
     1561 agttccgccg ggtcatactt cggcgcctcc aaagggcgca gcgacaaccc caccgtcttg
     1621 gtgcggttca gcccagcgac gatgcggcgg cagatcgaca gcgcatgatg gtcgttgcgg
     1681 gcatagtgat ccaccacgcc cgatgtccgc gcatgcacgt ccgcgccgcc cagatcctca
     1741 gcggatacga tctcgccggt cgcggcctgt accagcggcg gccccccgag gaagatggtg
     1801 ccctgcttgc ggacgatgat gctctcatcg ctcatggccg gaacataggc gccgcccgcg
     1861 gtgcatgatc ccatgaccac ggcgatctgc ggaatgccct cggccgacat gttcgcctgg
     1921 ttgaagaata tacggccaaa atgctcccga tccgggaaga tctcgtcctg catcggcaga
     1981 aacgcgccgc cggaatccac cagatagaca cacggcagat tgttcttccg cgcgatctcc
     2041 tgcgcgcgaa gatgtttctt caccgtcatg ggaaagtagg tcccgccctt gacggtggca
     2101 tcgttggcga cgatgacgca ttctcgtcca gacacacgcc caacgccggt gacgatcccg
     2161 gccgccggca catccccgcc atacagcccg aacgcggcga gttgcgaaaa ctccagaaac
     2221 ggcgatccgg aatcgatcaa cgccgccacg cggtcgcgcg gcagcagctt gccgcgcgcc
     2281 acgtgccgat cccgggcagc cgtgcctcca cccaaggaga tttctgcgac cgtttcccgc
     2341 agatcggcga cgagacctcg catcgattcg gcattcgccg aaaactcggc ggagcgtgta
     2401 gagatatcgg aacggatgat ggtcatggta cgaccggacc gccacc
//
LOCUS       DASWQN010000399         4780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1358183, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000399 DASWQN010000000
VERSION     DASWQN010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358183"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..706)
                     /locus_tag="VGC82_06535"
                     /pseudo
     CDS             complement(1..706)
                     /locus_tag="VGC82_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(973..1956)
                     /locus_tag="VGC82_06540"
     CDS             complement(973..1956)
                     /locus_tag="VGC82_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEY0422966.1"
                     /translation="MTDTLVVTFSVRPPERGAISDAIGDAAQVLYLTDLDEAGRILAL
                     RDATAILARDTDRDLHENEAAEIRSARLIQFITAGIDFVKLSALPPGIPVASNGGAYA
                     EPMAEHGLAMTLAAAKRLMTEHAALARGEFNQQKRNRTLSGKVCGILGFGGIGVASAR
                     LMRAMGMRIHAINRRGWSDEPTDWIGSVSDLDALLAVSDVLLVCTPLTPATCNLIGAR
                     QLGLMKPDAILVNLARGEIIQEAALFDHLRSHPEFFACIDAWWVEPVRHGAFRTDYPF
                     LTLPNVLGSPHNSASVPGMTGNALRRAVTNIRRALAGEKPHYVLGDDERMR"
     gene            complement(2015..2395)
                     /locus_tag="VGC82_06545"
     CDS             complement(2015..2395)
                     /locus_tag="VGC82_06545"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HEY0422967.1"
                     /translation="MKPLLALAMIASSLIGSGAVGSARAAQPISLHARTSGDLAELCG
                     ANPKEPAADAKLNYCDGFAQAVVDLELRHAGDKKPFCFPSPTPPRRVTLNEFAAWVRS
                     LPEHRTLSAPDGLLQFLGVRFPCK"
     gene            complement(2421..3143)
                     /gene="queC"
                     /locus_tag="VGC82_06550"
     CDS             complement(2421..3143)
                     /gene="queC"
                     /locus_tag="VGC82_06550"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018980502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY0422968.1"
                     /translation="MTQRSQTEDTALVLFSGGQDSTTCLAWALKRYCRVETVGFTYGQ
                     RHAVEMDCREPIREALSSPALGADHVVDLTATVAGLGQTAMTADIAIEMDHSGLPNTF
                     VPGRNLLFLTSAAAIAYRRGLRRIVIGVCETDYSGYPDCRDDTIKAMQLALNLGMQRR
                     FVLETPLMWLDKAATWALAAQLGGQDLVDAIRERTHTCYLGNRTDRHAWGFGCGTCPA
                     CLLRAQGWDRWQAAGRPHSDPI"
     gene            3188..4006
                     /gene="moeB"
                     /locus_tag="VGC82_06555"
     CDS             3188..4006
                     /gene="moeB"
                     /locus_tag="VGC82_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY0422969.1"
                     /translation="MDLDFTEDEIQRFSRHILLQEVGGTGQAKLKAARVLVVGAGGLG
                     SPLVLYLAAAGIGTIGIVDDDVVDLSNLQRQVAHTTDRIGMQKVASAAAAAAAINPDL
                     RVEMHPHRLSVANALELIDGYDIVCDGTDNFSTRFLIADACALAKRTLVSAAVLRFEG
                     QLSVFKPHEGGPCYRCLYPEPPPPGTVPTCSEAGVLGAVTGVMGTLQATEVLKEILGI
                     GDTMSGRLLVWDALATRFRNIKLRPDPSCALCGPNATIKDLNAHRNPVGPACAI"
     gene            3996..4379
                     /locus_tag="VGC82_06560"
     CDS             3996..4379
                     /locus_tag="VGC82_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007397311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEY0422970.1"
                     /translation="MPSDSTLGILLLSGMHDRAHYAFILASGAAALGRDVVLFATNLG
                     CVALCRDWSGLSDADRDVRIQARGVAGLDTLRDACVELNVRMIACEAGLRAEAVDASL
                     LLPGIEVAGVATFLSAIGSGQIITI"
     gene            4435..>4780
                     /locus_tag="VGC82_06565"
     CDS             4435..>4780
                     /locus_tag="VGC82_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422971.1"
                     /translation="MRLRVWDILLSVWLVAAGYGPVRAQTGSLLGTSQVPANPAHDQA
                     VKPHSAAPKMSGQKPDSAKSENPPTAHPPTAHSPVAHPSGAHPPVVAAHGPTHPQKPA
                     AVAKPSPPVPSPA"
BASE COUNT          845 a         1527 c         1526 g          882 t
ORIGIN      
        1 tccaccatgc ggcagatggc attgccccgc ggcgagtccg gagcgaagtc ctgccgctcc
       61 gcctcttccg gggatacgcc cagctggccg gcgcgggcgg cgagcaagct cggcgtgcgc
      121 tcggtgcggg tcgtgccggg atggatgcaa ttgaccgtga ccccatggcg gcccaattgg
      181 accgccagcg tcttggtcat gtgcacgagg gaggcgttgc gtgcgccgcc gctgagattg
      241 ccggcgttgc gggcgttggc accgctgatg ttgataatgc ggccccagcc ctgctgcttc
      301 atgaacggaa tgacagcgcg gcaacaggca gagctccgac atacttcacg ttgaagtcct
      361 gcaacaaatc gtcgtcgatg accgtttcga tcgggccggt cgcggtggcc gaaccccctg
      421 gcgaggatcc gctgttaacc agaatgtgca ggccgcccag ttgctgtgcc gcttcggcga
      481 ccatccgatc cacctgctct ttgctggtga cgtcggccgc gaggggaagg atgcgccggt
      541 tggtctcggc tcccagctcg cgcgccgtcg cctcgagagc gtccttgttg cgcgccacga
      601 tcgccacatc gacgccttcg cgcgccaatt cccgagcgat ggctttgccg atgccccgac
      661 tgccgccggt tacgatcgca tgcttgcctt gcaatccgag gtccatggca ttctctcccc
      721 ttggcggcac gaccgcgatt ttattgcttc actattcatt gttctctctg agggacgctg
      781 gcgcaagggg ttccagaggc agggcgatgt actgccgcga ttggtcgaac gctggatttg
      841 aggacggcaa ggcggcctgg aaggactgac ttgggcgcga agacgccgtc atggaaacaa
      901 gcatttcaga ccggcttccc agccttgtct gaccttcaaa ccgacccgct accaagtagc
      961 ctgggtcctt cgctatcgca tgcgttcgtc gtctcccagg acataatgcg gcttctcgcc
     1021 ggccaaggcc cgcctgatgt tggtgactgc ccgccgcagt gcattccctg tcattcccgg
     1081 aaccgacgct gaattatgcg gcgaacccag cacattcggc agcgtcagga atggataatc
     1141 cgtccggaac gcgccatggc ggaccggttc gacccaccag gcatcgatgc aggcaaagaa
     1201 ttccggatgc gaccgaagat ggtcgaagag agctgcttcc tgaatgatct caccgcgcgc
     1261 cagatttacc aggatggcgt ccggcttcat caggcccaat tgcctcgcgc cgatcagatt
     1321 gcaggtcgcc ggcgtcagcg gcgtacaaac cagcagcaca tccgacaccg ccagcagagc
     1381 atccaggtcc gacaccgaac cgatccagtc ggtcggttca tcggaccagc cacggcggtt
     1441 gatggcgtga attctcatgc ccatcgcccg catcaaccga gcggaggcaa ccccgatgcc
     1501 gccgaagccg aggatgccgc ataccttgcc ggacagggtg cggttacgct tctgctggtt
     1561 gaactcgccg cgcgccaaag ccgcatgctc ggtcatcagc cgcttcgcgg cggccaaggt
     1621 cattgccagc ccgtgttccg ccatcggttc ggcataggcg ccgccgttgc tggcgaccgg
     1681 aatgccgggc ggcaaagcac tcagcttcac gaagtcgatg ccggcggtga tgaactgaat
     1741 aagccgagcc gatcggattt ccgcagcctc gttttcgtgc aggtccctgt cggtgtcacg
     1801 ggccaggatc gcggtagcgt cgcgcagggc gagaatgcgg cccgcctcgt caaggtcggt
     1861 caggtagaga acctgcgcgg catcgccgat cgcgtcggag atggcgccgc gttccggcgg
     1921 gcgaacagaa aaagtaacta ccagcgtgtc tgtcataggt ccgagcatac ggcgtgcggg
     1981 ccatgctgtc tcagcatccc gaggactcca cggcctattt acagggaaag cgcacgccca
     2041 ggaactgcaa cagcccgtcg ggcgcggaca aggtgcggtg ttcgggcagc gagcggaccc
     2101 aggcggcaaa ttcgtttaac gtgacacgcc gcggcggcgt aggggaggga aagcagaacg
     2161 gcttcttgtc tcccgcatgc ctcagttcca ggtccacgac cgcctgagcg aaaccgtcgc
     2221 agtaattaag cttggcatcc gccgccggct ccttcgggtt ggccccgcat agctcggcga
     2281 ggtcgccgga cgtcctcgca tgcagcgaga ttggctgcgc cgcccgggca ctgcctacgg
     2341 cgcctgatcc gatcaagctg gatgcgatca tagcgagggc gagaagcggc ttcattggat
     2401 ggtctcctaa atcaggaaga tcagatggga tcggaatgcg gacggccggc agcctgccag
     2461 cgatcccaac cctgggcacg caacaggcag gccggacatg ttccacagcc gaatccccat
     2521 gcgtgacgat cggtgcggtt gccgagatag caggtatggg ttcgttcgcg aatagcatcc
     2581 acaagatcct gtccgcccaa ctgcgccgcc aatgcccagg ttgcggcctt gtcgagccac
     2641 atgagcggcg tttccaggac gaaccgccgc tgcatcccca gattgagcgc gagctgcatc
     2701 gccttgatgg tatcgtcgcg acaatccgga tatccggagt agtccgtttc gcacacgccg
     2761 atcacgatgc ggcgcaggcc gcggcgatag gcgatcgcgg cggcgctggt cagaaacagc
     2821 agattccgcc cgggcacgaa ggtgttcggc agtccgctat ggtccatctc gatcgcgatg
     2881 tccgccgtca tcgcggtctg gccgagcccg gcgaccgtag cggtcagatc aacgacatga
     2941 tcggcgccga gggcaggtga agacaatgcc tcgcggatcg gctcgcggca atccatctcc
     3001 accgcgtggc gctgaccgta agtaaaaccc acggtttcca cccggcaata gcgcttcagc
     3061 gcccaagcca ggcaggtggt cgaatcctgt ccgccggaga acagcaccag ggcggtgtcc
     3121 tcggtctggc tgcgttgcgt catcgatccc ctgtccagtc gtctcgtggt cacctattat
     3181 cagaagcatg gacctcgatt tcacggaaga cgaaatccag cgcttctccc gccatatcct
     3241 gttgcaggaa gtgggtggca ctggccaagc caagctgaag gcagcccgcg tgctggtggt
     3301 tggcgcaggt gggcttggct ccccgctggt gctgtatctg gcggcggcgg ggatcgggac
     3361 gatcggtatc gttgacgacg acgtggtgga tctgtcgaac ctgcagcggc aggtggcgca
     3421 tacgaccgat cggatcggaa tgcaaaaggt cgcctcggcg gctgcggcgg ctgcggcgat
     3481 caatccggac ctacgggtcg agatgcatcc ccaccgtctt agtgttgcga atgcgctgga
     3541 actgatcgac ggctatgaca tcgtctgcga tggcaccgac aatttcagca cgcggtttct
     3601 gatcgcggac gcctgtgcgc tggcgaagcg taccctcgtt tccgccgccg tgctgcggtt
     3661 cgagggccag ctttcggtgt tcaaaccgca cgaaggcggt ccctgctacc gatgcctgta
     3721 tccggagcct ccgccgcccg gcaccgtccc gacctgcagc gaggccggcg tcctgggagc
     3781 tgtcaccggc gtgatgggta ccttgcaggc gacagaggtc ctgaaggaaa ttctcggcat
     3841 cggcgacacg atgtcgggcc gtttgctggt ctgggacgcg ctggcgaccc gcttccgcaa
     3901 catcaagctg cgaccggatc cgtcgtgcgc gctgtgtggc cccaacgcga caatcaaaga
     3961 cctcaacgcg catcgcaacc cggtaggacc ggcttgtgcc atctgattcg acacttggca
     4021 tactgttgct gtcgggcatg cacgaccggg cgcactacgc cttcatcctc gcatcgggcg
     4081 cggcggcgtt gggccgcgat gtcgtattgt ttgccacgaa tttgggctgc gtggcgctgt
     4141 gccgcgactg gtcgggcctg tccgatgccg atcgggatgt tcgcatccag gcgcggggcg
     4201 ttgccggcct ggatacgctc cgcgatgcct gcgtcgaatt gaacgtcagg atgatcgcct
     4261 gcgaggctgg cttgcgtgcc gaagccgtgg acgcttcgtt gctgttgccg gggattgagg
     4321 tggcgggggt cgcgacgttc ctgtccgcca ttgggtctgg gcagatcatc acaatttgag
     4381 ccgcaggcaa ccatgagctt gacccgattc cggccaactt ggcatggatg cggcatgcgg
     4441 ctgcgagttt gggatattct gctctcggtg tggctcgtgg cggccggtta tggaccggtc
     4501 cgagcccaaa ccggctccct gcttggcacc tcgcaggttc cggccaaccc ggcgcacgat
     4561 caggccgtga agccgcattc cgcggcgcca aaaatgtccg ggcaaaagcc cgattccgcg
     4621 aaatcggaga atccgccaac cgcacacccg ccaaccgcac actcgccggt cgcgcatccg
     4681 tctggcgcgc atccgccggt cgtcgctgcg catggaccga cgcatccgca aaaaccggct
     4741 gccgttgcca aaccatcgcc gccggttcca tccccggcac
//
LOCUS       DASWQN010000400         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1886418, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000400 DASWQN010000000
VERSION     DASWQN010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1886418"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VGC82_06570"
     CDS             complement(<1..778)
                     /locus_tag="VGC82_06570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0422972.1"
                     /translation="MTNVILITRLSGAYDGPNAMAGPNVMARLFRGRLHYAWVVVAVM
                     FVVILASVGVRATPGVLIVPLQEAFGWNAAVISGAVSLNILLFGLVGPFAAGLIQVIG
                     LKRTVLCSMVLLVIGAGSSAFVGEIWQLYLTWGVLVGLGSGAGMVGLATAIANRWFVH
                     RRGLVVGLLTASNASGQLVFLPLLASLVVSHTWHIVPLVVAGVILALIPLVLLMLPES
                     PASIGIGPFGSKEIVQLPPPSGNPFAIAMAGLIRGAKSGDF"
     gene            829..1431
                     /locus_tag="VGC82_06575"
     CDS             829..1431
                     /locus_tag="VGC82_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0422973.1"
                     /translation="MAEPTSKRAETRHRILTAAGQLFREHGIDGVGVDAVMKEAGLTH
                     GGFYLHFPSKEALAAEVAQSLLSSAAAGWDDISRRPDRAAALERIVRYYLDPDRVASA
                     HCCPLTTLGPDMARRPTSKEAVAGALRGMLDALARVLPGRKRQKALTSLSTMVGAVVL
                     SRLADDPALAEAFLNAAAASILPAGAAVGGSNVARDQLDH"
     gene            1720..3561
                     /locus_tag="VGC82_06580"
     CDS             1720..3561
                     /locus_tag="VGC82_06580"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0422974.1"
                     /translation="MATDIWKATVTSVSGNWSDATKWTPSAPLSGDQIIIGNTTQKNP
                     FTVTEDVTLTIGSLTMAGKHAGNQTTTLLLTQPAVLTVNGPISLDLDAIIAGSGTLVA
                     NGAITGTGTILASNGLIDITGTGSIANGVVLDFINTGTLASTLKLDLSGGVTSAANIK
                     MSNAAQTLEVGANTSLTILAAETVANGTLRMSGGTVTDTLGITVGGQGTPGTIIGFGT
                     IAANLIGGGAKATTDTVTASGGMLDLIGSFSAGAATFLAKIDSTSASTLKFDGNAIVG
                     QPITINSAFQTLEIGALGTLTMTGLDIVTNGTIKMDGGMLADTAGIVIDTAATLTGFG
                     LVAAGTTLSGDGIIKASGGMLELASDLTASTTLFDVDNVAGSVLKVDGAVASVATLSF
                     LGATGALELADVTAGVLQGFSGKIAGLNVGASATIPTNAINIQAVVSKAVLAGSTLTI
                     FNGSTIVAALALSATPVQGAYAVFRANATLGGYDVFLSNEPPVAPSAPALAAGSDSGT
                     KGDRITNIATPVFTGTGVTGDTVTLFDGTTAVGSATVDVNGNWSIATAALPDGLNVIT
                     ARQSDLLGNLSAPSVSVTITVDTLAPGVTVALASDTGASPIDHITRN"
BASE COUNT          732 a         1189 c         1097 g          633 t
ORIGIN      
        1 agaagtcgcc cgatttcgca ccacgtatca gcccggccat tgcaatggcg aagggattgc
       61 cggacggcgg cggcaattgc acgatctcct tggatccgaa tggcccgatc ccgatcgacg
      121 cgggactttc cggcaacatc aggagcacca ggggaatcag cgcgaggatt actccagcca
      181 caaccagcgg cacgatgtgc caggtatggg agaccaccag gctcgccaga agcggcagga
      241 acaccagttg gcccgaagca ttgctcgcgg tcagcaagcc taccaccagt ccgcgccggt
      301 gaacgaacca acggttggcg atcgcggtcg ccagtccgac catcccggcg cccgacccca
      361 agccgaccaa gacgccccag gtcaggtata gctgccagat ctcgccgacg aaggcgctac
      421 tgcccgcgcc gatcaccagc aagaccatcg agcacagcac cgtccgcttc aacccgatca
      481 cctggatcag cccggcggcg aacggaccta ccagtccgaa cagcagaatg ttcaacgaca
      541 ccgcgcccga gatcaccgcg gcgttccagc caaacgcttc ctgcaacggc acgatcagca
      601 cgcccggtgt ggcccgcacc ccgacggacg ccaggatgac gacgaacatc accgccacga
      661 ccacccaggc gtaatgcagg cgccccctaa acaggcgcgc catcacgttc ggtcccgcca
      721 tcgcgttcgg tccatcgtaa gctccggaaa gtcttgtgat caggatgacg ttcgtcatat
      781 gaaagtcaag tgctaatatg acgatcgtca caagtagcga ggcctatcgt ggctgaaccg
      841 acctccaaac gcgcggaaac ccgccatcgc atcctgaccg cggccggcca gttgttccgt
      901 gaacacggga tcgacggcgt tggcgtggac gcggtgatga aggaggccgg gctgacccat
      961 ggtggcttct accttcactt cccgtccaag gaagcgttgg cggccgaggt cgcgcaatcc
     1021 ctgctgtcga gcgccgccgc cggctgggac gacatcagcc gccgtcccga ccgggctgcg
     1081 gcgctggagc gtattgttcg ctactatctc gatcccgacc gggtcgcttc agcgcattgt
     1141 tgtcccctga cgacgcttgg gcccgacatg gctcgccggc ccacgtcgaa agaagctgtc
     1201 gcaggcgcgc tgcgagggat gctggacgcg ttggcacggg tgcttcccgg gcggaagcga
     1261 cagaaggcgc tgacgtcctt gtccaccatg gtcggtgctg tggtgctgtc acgcctggct
     1321 gacgatccgg cactggcgga agcttttctg aacgcggcgg cagcaagcat tttgcctgcc
     1381 ggcgcggcgg tcggtggctc gaacgtggca agagaccaac tggaccatta accggcggcg
     1441 gcaaatcccg cggccgaagc aactgatcct ccaaccagag gagcgcgccg tttcgtcgga
     1501 cagtcgccaa gggcggtctt atgggtttgc cggcgaaggg cggacgccga ttactacagt
     1561 tgatctcatt tcattgccaa accttgcagt agtctctttt tcgttctgat agcatcttta
     1621 ttacttttac aatttagtat tttatacgaa acgtatttta acagggcgtc tgatcgggcg
     1681 gcttcggatc aacatacata agcaagggac gaactcgtca tggcaacgga tatttggaag
     1741 gcaacggtca cctcggtcag cggcaactgg agcgacgcga ccaagtggac gccgtcagca
     1801 cctttgtccg gcgaccagat catcattggg aataccacac agaagaatcc ctttacggtc
     1861 accgaagacg tcaccctgac catcggctcg ctcaccatgg caggcaaaca cgcgggcaat
     1921 caaaccacga cgctgctgct gacccagccg gcggtactca ccgtgaatgg cccgatcagc
     1981 ctggacctgg acgcgatcat cgccggcagc gggacgctgg tcgcgaacgg cgcgatcacc
     2041 ggaaccggga ccatccttgc cagtaacggg ttgatcgaca tcaccggcac cggctcgatc
     2101 gcgaatggcg tggttctcga cttcatcaat accggcaccc tcgcttccac gctgaagctc
     2161 gacctgagcg gcggcgttac gtccgcggca aacatcaaaa tgagcaatgc ggcgcagacg
     2221 ctcgaagtgg gcgccaacac cagcctgacg atcctcgcgg cagagaccgt cgccaacggc
     2281 acgttgcgga tgtcgggtgg cacggtcacc gacacgcttg gaatcaccgt gggtggccag
     2341 gggactcccg gcaccatcat cgggtttggc acgatcgccg cgaaccttat cggcggcggc
     2401 gccaaggcaa cgaccgacac cgtcactgcg tcaggcggta tgcttgacct gatcgggtcg
     2461 ttcagcgcgg gcgcggcgac atttttggcg aaaatcgact cgacatctgc ctcgaccctg
     2521 aagttcgacg gcaacgcgat cgtgggccag ccgatcacga tcaacagcgc ttttcagacg
     2581 ctggaaatcg gcgctcttgg caccttgacg atgaccggcc tcgacatcgt caccaacggc
     2641 accatcaaga tggatggcgg catgctggcc gataccgcgg ggattgtcat cgacaccgcc
     2701 gcgacgctga ccggcttcgg gttggtcgcg gccggcacga cgctgtccgg cgacggtatc
     2761 ataaaggcca gcggcggcat gctggagctt gccagcgacc tgacagcctc gacgacgttg
     2821 ttcgacgtcg ataacgtggc cggttcggtg ttgaaggtcg atggcgcggt cgcatcggtc
     2881 gcgacgcttt cgttcctcgg tgcgaccggg gcgcttgaat tggccgacgt gaccgccggc
     2941 gttctgcagg gcttcagcgg caagatcgcc ggcctcaacg tgggcgccag cgcgaccatc
     3001 ccgaccaacg ccatcaatat ccaggcggtg gtttcgaagg ccgtgctcgc gggaagcacg
     3061 cttaccatat tcaatggcag caccatcgtc gccgcgcttg cgctgagcgc cacaccggtt
     3121 caaggcgctt acgcggtgtt ccgggccaat gccacgctcg gcggctatga cgtgttcctc
     3181 agtaacgagc ctccggtagc accgagcgct ccggccttgg cagcgggaag cgacagcggc
     3241 accaaagggg accgcatcac caacatcgcc accccggtct ttaccggcac gggggtgacc
     3301 ggtgacaccg ttaccttgtt cgatggcacg acagcggtcg gctccgccac cgttgacgtc
     3361 aacggcaact ggtcgatcgc caccgcggcg ctgcccgatg gtctgaacgt tatcaccgcc
     3421 aggcaatccg acctcctcgg aaatctctcg gctccttccg tttccgtgac gattactgtt
     3481 gacaccttgg cgcctggcgt gaccgtggcc cttgcgtccg ataccggtgc gtcgccgatc
     3541 gaccacatca cccgcaatta ggctctgacc ggttcggggg acgccaacag cacagttacc
     3601 atcaaaaacg gcgccattgt acttggcgca acggttgccg atgcaagcgg c
//
LOCUS       DASWQN010000401         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_302890, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000401 DASWQN010000000
VERSION     DASWQN010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302890"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VGC82_06585"
     CDS             complement(<1..422)
                     /locus_tag="VGC82_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422975.1"
                     /translation="MNPRIGRWVWRLLPALPASILLLAWSVWFAIDRPPASVMPSAPD
                     VVVTMFDLAVHGRLAGDIVASLGRLLSGVAVGVTTGIVAGFVAGLNRPVATFLNPLVV
                     FFNAISGIVWLPLVIGWLGIGSALAIFLIWNTVFFIVF"
     gene            complement(419..925)
                     /locus_tag="VGC82_06590"
     CDS             complement(419..925)
                     /locus_tag="VGC82_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0422976.1"
                     /translation="MPREHLKARCDFFRGSRGIAWLPLAILWFGFTNTTTLTIVIYTL
                     IVPIVFNTMTGVRAIPENYALALQSLGAGRLRIVWDVYLPGALPSIVVGMRLGMGYGW
                     RALIASEMLVRQGGLGDLIFGARTFGQIDRIMVGMVVIGTLYIVVDRLLVQPVETMTL
                     ARWGVLRR"
     gene            complement(1184..1957)
                     /locus_tag="VGC82_06595"
     CDS             complement(1184..1957)
                     /locus_tag="VGC82_06595"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0422977.1"
                     /translation="MADKIEVSGFRHFFRTEAGPVQATGLIDLSIRPGEVVTLVGPSG
                     CGKTTLLKAIAGFLTPSEGRILCDGKPVRGPGRERGVVFQELAILPWRTVRRNIAHGL
                     EIARTSRTERDATVARLVGLMGLRGFEDRYPHELSGGMRQRVAVARTWAADPEVILMD
                     EPFAAVDAMTRLTLQEELGRLCAATRKTVFFITHSVEEAVFLADRVLVMTKRPGTIKA
                     VLEVPRSAAGGRDWDSFTSDPAMQKIAGEVMHLVHAERV"
BASE COUNT          440 a          792 c          576 g          314 t
ORIGIN      
        1 tggaatacaa tgaagaaaac cgtgttccag atcaggaaaa tggccaaggc actgccaatt
       61 cccagccacc cgatgacaag cggcagccag acgatgccgg agatggcatt gaaaaacacc
      121 accagcggat tgaggaacgt agcgaccggc cgattaagtc ccgccacgaa tccggcaacg
      181 atcccagtcg tgaccccgac cgcgaccccg ctcagaagcc gccccaggct ggcgacaata
      241 tcgcccgcca gccgcccatg aaccgcgagg tcgaacatcg tgacgaccac atccggtgcc
      301 gagggcatca ccgacgccgg cggccggtcg atggcgaacc acaccgacca cgccagcagc
      361 aaaatcgatg ccgggagcgc cggcaacagc cgccaaaccc atcggccgat ccgcgggttc
      421 atcgccgcag cactccccag cgtgccagcg tcatggtttc gaccggctgc accagcaacc
      481 ggtccacgac gatgtacagc gtgccgatca ccaccatccc gaccatgatc cggtcgatct
      541 gcccgaacgt cctcgcgccg aaaatcagat cgcccaggcc gccttgacgc accagcatct
      601 cggacgcgat cagcgcccgc cagccatacc ccatccccag ccgcatcccg accacgatgc
      661 tcggcagcgc ccccggcaaa tacacgtccc agacgatgcg caaccgcccc gcgcccaggg
      721 actgcagcgc cagcgcatag ttctccggga tcgcccgcac gccggtcatg gtgttgaaca
      781 cgatcggcac gatcaacgta tatatcacga tcgtcagcgt ggtcgtattg gtgaagccaa
      841 accacagtat cgccagcggc agccaggcaa tcccccgaga tcccctgaag aaatcgcagc
      901 gtgccttcaa gtgctcgcgc ggcataacgg ttggtcccga cagcaaaacc cagcggcacg
      961 ccgacgacga aggaaatcac cgcggcggtt ccgatcatcc gcaggctggc actggcatat
     1021 tccgccagga tcccgtgcgt gaccaggccg gtgaagctgg cccagacggc gggcggagcc
     1081 accagcagct tgtccggcac atcgccgcca atcttgatgc cccaccagat ggcgatcagc
     1141 accgcgaacg gcagcagcac gcgaaacgac gccagaacct tcactacacg cgctccgcat
     1201 gcaccagatg catcacctcg ccggcgatct tctgcatggc cgggtccgac gtgaaactgt
     1261 cccagtcccg cccgcccgct gccgaccgag gcacctccag caccgccttg atcgttcccg
     1321 gccgcttcgt catcaccagt accctgtcag cgagaaacac cgcttcctcg acgctatgcg
     1381 tgatgaagaa caccgtcttg cgggtcgccg cacacagccg cccaagctcc tcctgcaatg
     1441 tcagccgggt catcgcgtcc accgccgcga acggttcatc catcaggatc acctcgggat
     1501 ctgcggccca ggtgcgtgcg accgccaccc gctggcgcat gccgccggac agttcatgcg
     1561 gataccggtc ctcgaacccg cgcagtccca tcagcccgac caaccgagcc accgtcgcat
     1621 cgcgttctgt tctggaagtc cgagcgattt caagcccatg ggcgatgttt cgccgcaccg
     1681 tccgccacgg caggatcgcc agttcctgga acaccacgcc gcgctcccgt cccggacccc
     1741 gcaccggctt gccatcgcat aggatgcggc cttcactcgg tgtcaggaac cccgcgatcg
     1801 ccttcagcag cgtcgtcttg ccgcagccgc tcggtcctac cagagtaacg acttcgccgg
     1861 gccggatgga caggtcgatc agcccggttg cctgcaccgg cccggcttcc gtccggaaga
     1921 aatggcggaa gcccgagacc tcgatcttgt cggccattct aaccgtccag cgcgcccttg
     1981 cagtcttcca gacgcgtcgg cagtggcggc aagccttcaa acagatccgg gcgcttcgcc
     2041 ttcacccgtt cataggccgt catgtcgtag aaatccgccg cgacgaacgg ccggtccatc
     2101 ttgatctgac cggacttcac ca
//
LOCUS       DASWQN010000402         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2413651, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000402 DASWQN010000000
VERSION     DASWQN010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2413651"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..972
                     /locus_tag="VGC82_06600"
     CDS             274..972
                     /locus_tag="VGC82_06600"
                     /inference="COORDINATES: protein motif:HMM:NF017855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEY0422978.1"
                     /translation="MAEYVVEFGKDGRPGEPDGRLDAPAFHRNHEAIWTVLEPFLAGK
                     SGDVVETGSGTGQHVVDFARRSPEITWWPSDLNEQHLKSIAAWRAHAALTNVRPPLRI
                     DLSDPAWCPELHDGSGPGKLLAVFCANVIHIAAWRVTEGLFAGAGRYLRADGRLFLYG
                     PFKRDGQHMAASNAAFDASLRERNPEWGVRDISDLEKLAGAAGRVLAGTAQMPANNLT
                     LVFGRSKTTPEHDA"
     gene            1075..2322
                     /locus_tag="VGC82_06605"
     CDS             1075..2322
                     /locus_tag="VGC82_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0422979.1"
                     /translation="MIDLTATDEMASDARARANVARLGAAQALTGANSAVIFATGSIV
                     GAGLAPDISLATVPLSMYVLGLAAGTLPTGAISRAYGRRVAFIIGTGCGVLTGLLGAF
                     AILRGSFALFCCATFLGGLYGAVSQSYRFAAADGASAAFRPKAVSWVMAGGVFAGVLG
                     PQLVQWTMDIWPPYLFAFSFMVQAAVALVAMAILAGVDAPKPAPADLHGGRPLLQIVR
                     EPRFIAAALCGVISYPMMNLVMTSAPLAMKLCGLSVSDSNFGIQWHIVAMYGPSFFTG
                     SLIARFGAPKIVAVGLLLEAAAATIGLSGITALHFRATLIVLGLGWNFGFVGASALVL
                     ETHRPQERNKVQAFNDFLVFGMMAVGSFSSGQVLANYGWSAVNLVVYPPVLLGLTVLT
                     LASFARRRRARPHPLAEFPDPRI"
     gene            2431..>2621
                     /locus_tag="VGC82_06610"
     CDS             2431..>2621
                     /locus_tag="VGC82_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422980.1"
                     /translation="MGHSSGTSIDETSARYDGWRVVAVCFLVATFGWGLGFYGQSIYL
                     AELHRLHGWPASLISSATT"
BASE COUNT          406 a          806 c          887 g          522 t
ORIGIN      
        1 acgccgatca gcagcgccag cgcgagcgcc gggaacagca cgtggcaggt tgcggaggtg
       61 tcggtcaaag gcgcttcctg gtcgtttgtg tcgcttgccc cgagaatata agaggtgaaa
      121 gcataaacgc catcggctga caacgtcgat cggcgattgg aaaccgccac ataaggaatt
      181 tctcgatacc ggagaaagcc tgatttacgt taacattact ttggcgggcg gtgcggtcgt
      241 ggctcgaagc cgaagcttca tccggagcaa gagatggccg aatacgtggt ggaattcggc
      301 aaggacggcc ggccggggga gcccgacggc cgtctcgatg cgccggcgtt tcaccggaac
      361 cacgaggcga tctggacggt tctggagcca ttcctcgccg gtaaatccgg cgatgtggtc
      421 gagaccggca gcggcaccgg ccagcacgtg gtcgatttcg cccggcgcag ccccgagatc
      481 acctggtggc cgagcgatct caacgagcag catctgaaaa gcatcgcggc gtggcgggcg
      541 catgcggcgc tgacgaatgt ccgtccgccg cttcggatcg acctttccga tccggcgtgg
      601 tgtcccgaac tgcatgacgg cagcggtccc ggaaaattgc tggcggtgtt ctgcgccaat
      661 gtcatccaca tcgcggcgtg gcgggtgacg gaaggattgt ttgccggcgc cgggcggtat
      721 ctgcgcgcgg acgggcggct gttcctgtat ggcccgttca agcgggacgg ccagcacatg
      781 gcggccagca atgcggcgtt cgacgccagt ctgcgtgaaa ggaatcccga atggggcgtg
      841 cgcgacatct ccgatctgga gaagctggcc ggcgccgccg gccgggtgct ggccgggacg
      901 gcgcagatgc ctgccaacaa tctgacgctg gtattcgggc gatcgaagac aacgcctgaa
      961 cacgatgcat agcctgcgcc cgtttgcgcc ggttgatcgg gcatatggct cgcttcatag
     1021 tgacaggtat taataggccg gttgcacggc catgccgggg cgcaaacagc ttcaatgatc
     1081 gacctgaccg caactgacga gatggccagt gatgcccggg cgcgcgccaa cgtggcgcgg
     1141 ctgggggcgg cgcaggcgct gaccggtgcg aattccgcgg tgatcttcgc taccggctcg
     1201 atcgtgggcg cgggcctcgc gccggatatc tcgctcgcga ccgtgccgct gtcgatgtat
     1261 gtgcttgggc tggctgcagg caccttgccg accggggcca tctcgcgtgc ctatggccgc
     1321 cgcgtcgcct tcatcattgg caccggctgc ggcgtgctga ccgggctgct cggggcgttc
     1381 gcgatcctgc gcgggtcatt tgccttgttc tgctgcgcga cgttcctcgg aggcctctat
     1441 ggcgcagtgt cgcaatccta tcgtttcgct gccgctgatg gcgccagcgc cgcgtttcgt
     1501 cccaaggccg tgtcatgggt aatggcgggc ggcgtgttcg ccggcgtgct cggcccgcag
     1561 cttgtgcagt ggacgatgga catctggccg ccctatcttt tcgcgttttc cttcatggtg
     1621 caggcggcgg tcgcgctggt cgcgatggcc atcctcgccg gggtcgatgc gccgaagccc
     1681 gcgccggccg atcttcacgg cggccgtccg ctgctgcaga tcgtgcggga gccgcgcttc
     1741 atcgccgccg cgctctgcgg cgtgatttcc tatccgatga tgaacctggt gatgacgtcg
     1801 gcgccgctgg cgatgaaatt atgcgggctc agcgtcagcg attccaactt cggcatccag
     1861 tggcatatcg tggcgatgta tgggccaagc ttcttcaccg gttcgctgat cgcgcgcttc
     1921 ggcgccccga aaatcgttgc ggtgggcctc ctgctcgagg ctgccgccgc gacgatcgga
     1981 ctgtccggca tcacagcgct gcatttccgg gctacgctga tcgtgctggg cctgggctgg
     2041 aattttggct ttgtcggcgc ctccgcgctg gtgctggaaa cgcaccggcc gcaggagcgc
     2101 aacaaggtcc aggccttcaa cgattttctg gtgttcggga tgatggcggt ggggtcgttc
     2161 tcgtccggcc aggtgctggc gaattacggc tggtcggccg tcaatctcgt cgtctatccg
     2221 ccggtgctgc tcggattgac ggtgctgacg ctcgcatcgt tcgccaggcg gcggcgggcg
     2281 aggccgcacc cactggccga gtttcccgat ccgcgcatct gaatccgatg acatgacgct
     2341 tcggcgacgc tcgaagcgtt gcttccgttg gtccgctacc gtcatgcgga gacaccccga
     2401 caacgtgcaa aaaaactgaa gcggtagacc ttgggtcatt caagcggcac atccatcgac
     2461 gagacctcgg cccgttacga cggctggcgt gtcgtcgcgg tctgctttct ggtggcgact
     2521 ttcggctggg ggctcggctt ctacggccaa agcatctatc tcgcggagtt gcaccgcctg
     2581 cacggctggc ctgcctccct gatctcctcg gcaacgacat t
//
LOCUS       DASWQN010000403         3508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2150119, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000403 DASWQN010000000
VERSION     DASWQN010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2150119"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VGC82_06615"
     CDS             complement(<1..904)
                     /locus_tag="VGC82_06615"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422981.1"
                     /translation="MASKSNVYAGVAGYVGRADQKGSVGVFTRPAETGRWEHVLTDHE
                     TYTVNVHPTNPDVVFAGTADGVYRSTDRGKSFQRTNFPDKGVQIWSFLVDSENRNLVY
                     AGGSPISMYRSEDGGETWRALPNPGMPDRAVMPFACRVMRMAQHPSNPGTIFAALEVN
                     GVMRTNDGGESWSDCSKDLIRLAQQPHLKSKIVSDTYNEGMLDGHAIAINPADPDKVI
                     VAVRMGLFETEDGGNTWHDMEVGRFSPTTYGRDIRVSPDGSTLYAALSVAAASKDGGI
                     YRSTDKGASWKRFDKVQVHGTIMSI"
     gene            1022..2071
                     /locus_tag="VGC82_06620"
     CDS             1022..2071
                     /locus_tag="VGC82_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY0422982.1"
                     /translation="MPNVSAEKLIEIAKGLLVAAGASEEEAAVIARYNIGANLVGHDS
                     HGIILIPTYIDRIKAGHIVPQAPWVITQETPTTTVVDGNWGFGYAVSDRAMRYTIEKA
                     KKQNVAATTVFHQSHIGRLASYPLLAAAEGMIAMITADSGRSAKAVAPYGGAKARLGT
                     NPICFAIPSNLDGPLVFDMATSAAAAGKINVATARGEQVPAGWLIDAEGKPSTDPRVL
                     KSGGALLPLGGTEGYKGTGLAAIVEILSGLLTGLGFGVEPTGRHNDGCFMAVFNVAAF
                     RDLGVFKQEVTEFAQYLKATPLAQGFTEVYYPGEVEYKKEQDRRKNGIPVEDATWNKV
                     RELAQGYGLTDKLGL"
     gene            2072..>3508
                     /locus_tag="VGC82_06625"
     CDS             2072..>3508
                     /locus_tag="VGC82_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY0422983.1"
                     /translation="MTPFIGQSTRRLEDARFLTGRGTFVDDANDAGQARAFVVRSPHA
                     HATIDRIDTTAAKAMPGVLGIYTHDDISGLGLLPCATLVATVSPMIVPPRPALASGKV
                     RHVGDPVAFVVAETAMAARDAAEQVLVDYTALPCVVDAVSALAPGAPAIWDTGNFSYQ
                     FQRGDRTAVQAAFDAAAHVVDIEVVNNRLVVAPLETRAAIGRWADGVFDLFVSAASVH
                     AIRDQLASSVFRVPRDAVRVSAPDVGGGFGIKNCLYPEWVMLLWSARHLGRSVKWVED
                     RAEDFVSSAQGRDNVSRGRLALDADGRFLALDVSTVAALGAYMSGGGPGSSTNAPAAA
                     MGGGYVIPAIFMDVRAAFTNTVPIDAYRGAGKPEANYLIERLIDKAAAACGFDPVELR
                     RRNLIAAFPYQKALGAVVDCGRFASNIGDVLALADQGGYPQRHAASSAKGLVRGIGVA
                     CFLEAARGAPNEGAEIRFGDDGLVTLLVG"
BASE COUNT          599 a         1195 c         1086 g          628 t
ORIGIN      
        1 cgatcgacat gatggtgcca tgcacctgca ccttgtcgaa acgcttccag ctcgcgccct
       61 tgtcggtgct gcgataaatc ccgccgtcct tgctcgccgc cgccacactg agggccgcat
      121 aaagcgtgct tccgtccggc gaaactctga tgtcccgccc ataggtggtg ggtgagaacc
      181 ggccgacttc catatcatgc caggtatttc cgccatcttc ggtctcgaac aggcccatgc
      241 gcactgcgac gatcaccttg tccggatcgg ccgggttgat ggcgatcgcg tggccgtcca
      301 gcatgccttc gttataggtg tcgctgacga tcttgctttt caggtgcggt tgctgcgcca
      361 gcctgatcag gtccttgctg caatcgctcc agctttcgcc gccatcattc gtgcgcatca
      421 cgccgttcac ctccagtgcg gcgaaaatcg tgccggggtt cgacgggtgc tgcgccatcc
      481 gcatcacacg gcaggcaaac ggcatcaccg cgcgatcggg catgcccgga ttgggcagcg
      541 cgcgccaggt ttcgccgcca tcctcgctgc ggtacatgct gatcggcgac ccgccggcat
      601 aaaccagatt ccgattttcc gagtccacca ggaacgacca gatctgcacc cccttgtccg
      661 ggaaattcgt gcgctggaac gacttgccgc ggtcggtgct gcgatacacc ccgtcagccg
      721 tcccggcgaa caccacatcc ggattggtcg ggtggacatt gacggtatag gtctcgtggt
      781 cggtcaacac atgctcccac cgcccggtct ccgccggtcg cgtgaacact ccaaccgatc
      841 ccttctgatc cgctcggcct acgtatccgg ccacaccggc ataaacgttc gacttgctgg
      901 ccatgtttgc ctccccgctt ggattttcgt cggacaacca cgctacgacg gtttagccgt
      961 cctggccaac tcccggtgcc tgggttagcc tgccgcacat cggggaacgc ggagatcgtc
     1021 catgcccaac gtgtcagcgg aaaaactgat cgagatcgcc aaaggcctgc tcgtcgcggc
     1081 cggcgcgtcc gaggaagaag cggcggtcat cgcccgctac aacatcggcg ccaacctggt
     1141 cgggcatgac agccacggca tcatcctgat cccgacctat atcgaccgca tcaaggccgg
     1201 ccacatcgtc ccgcaggcac cctgggtcat cacccaggaa accccgacca caaccgttgt
     1261 cgatggcaac tggggctttg gctacgccgt aagcgatcgg gcgatgcgct acaccatcga
     1321 gaaggcgaag aagcagaacg tcgccgccac cacggtgttt caccagagcc atatcggacg
     1381 cctcgccagc tacccgctgc tcgccgccgc cgagggcatg atcgccatga tcaccgccga
     1441 ttccggccgc agcgccaaag ccgtcgcacc ctacggcggc gcgaaagcca ggctcgggac
     1501 caacccgatc tgctttgcca ttccgtccaa tctcgatgga ccgttggtgt tcgacatggc
     1561 gacgtccgcc gcggccgcag gcaagatcaa cgtggcgacc gcacgcggcg agcaggtccc
     1621 ggccggctgg ctgatcgacg ccgaaggcaa gccatcgacc gatccacgcg tgctgaaatc
     1681 cggcggtgcg ctgctgccgc tgggcggcac cgaaggctac aaaggcaccg gcctcgctgc
     1741 gatcgtcgaa atcctgtccg gcctgctgac cgggcttggc ttcggcgtcg aaccgaccgg
     1801 acgccataac gacggctgct tcatggcggt gttcaacgtc gccgcgttcc gcgacctggg
     1861 cgtcttcaag caggaagtga ccgaattcgc gcaatacctg aaggccacgc cgttggccca
     1921 aggcttcacc gaagtctatt atccgggaga agtcgagtac aagaaagagc aagaccggcg
     1981 caagaacggc attccggtgg aagacgccac ctggaacaag gtccgcgaac tggcacaagg
     2041 ctacggcctg accgacaaac tcgggctgta aatgacgccc ttcatcgggc aatccaccag
     2101 gcggctggag gatgcgcgct tcctcaccgg acgcggcact ttcgtcgatg acgcgaacga
     2161 tgcggggcag gctcgagcat tcgtggtccg ctcgccgcat gcgcatgcga cgatcgatcg
     2221 gatcgacacg acggcggcga aggcgatgcc cggcgttttg ggtatctaca cccatgatga
     2281 catctccggc ctcgggctgt tgccgtgcgc gacgcttgtg gcgaccgtgt cgccgatgat
     2341 cgtgccgccg cgtccggcgc tggcatcggg gaaggtccgt cacgtcggcg atccggtcgc
     2401 cttcgttgtc gccgaaaccg ccatggccgc tcgcgatgcc gcggagcagg tgctggtcga
     2461 ttataccgcc ctgccttgcg tggtcgatgc cgtctccgcc ctggcaccag gcgcgccggc
     2521 aatatgggac accggcaatt tttcatacca gttccagcgt ggcgatcgga cagccgtcca
     2581 agccgccttc gacgccgccg cgcacgtcgt ggacatcgag gtcgtcaaca accgcctggt
     2641 cgtcgcgccg cttgaaacca gggcggcgat cggccgctgg gcggacggcg tgttcgacct
     2701 gtttgtcagc gccgccagcg ttcacgccat tcgcgaccag cttgcatcca gcgtgttccg
     2761 cgttccccgc gatgccgtcc gcgtttcggc accggatgtc ggcggcggct tcgggatcaa
     2821 gaattgcctc tacccggaat gggtgatgtt gctgtggtcg gcgcgccatc tcggccgttc
     2881 ggtcaagtgg gtggaggacc gcgccgagga tttcgtatcc agcgcgcaag gccgggacaa
     2941 tgtgtcgcgc ggccggctgg cgctggacgc cgacgggcgt ttcctggcac tcgacgtcag
     3001 caccgtcgcc gccctgggcg cctatatgtc cggcggtgga ccgggcagtt ccaccaacgc
     3061 ccccgccgcg gcgatgggcg gcggctacgt tatcccggcg atcttcatgg atgtgcgggc
     3121 cgcgttcacc aacaccgtcc cgatcgacgc ctaccgcggc gccggcaagc ccgaggccaa
     3181 ctacctgatc gaacgcctga tcgacaaagc cgccgcggca tgcggcttcg acccggtgga
     3241 gcttcgccgg cgcaatctga tcgccgcgtt cccctaccag aaggcgttgg gcgccgtcgt
     3301 ggattgcggc cggtttgcca gcaacatcgg cgacgtgctg gccctcgccg atcaaggcgg
     3361 ttacccgcaa cgccatgctg ccagcagcgc gaagggcctg gtgcgcggta tcggtgtcgc
     3421 ctgcttcctg gaagccgccc gtggcgcccc gaacgaaggt gcggagattc gtttcggcga
     3481 cgatggcctg gtgaccctgc tcgttggc
//
LOCUS       DASWQN010000404         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1622514, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000404 DASWQN010000000
VERSION     DASWQN010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622514"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VGC82_06630"
     CDS             <1..240
                     /locus_tag="VGC82_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422984.1"
                     /translation="SPVMVAAAVAVAGVLAMVVVDHGPWSRPHPEAANLANHGSVDEA
                     ARAAGATVTPTEPKSDVEPAVAGPKRAQPVSPGAP"
     gene            512..>1597
                     /locus_tag="VGC82_06635"
     CDS             512..>1597
                     /locus_tag="VGC82_06635"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HEY0422985.1"
                     /translation="MNRRRFLQAAASLPLASPLAGGIISPAQADSSPFDRSIVRQMAR
                     DAAAKPFKAPETKLPDSLKKLDYDHYRAIRFLPDRALWRGEKLPFEVQFFHRGFFYAP
                     RVDIYEVANNQATRIVYRPEQFSFGNTPPPSPDADLGFAGFRLHAPINRPDYYDEVCV
                     FLGASYFRAVAKGELYGLSARGLAINTGEAKGEEFPQFRTFWIEKPAPNANSVVVHAL
                     LDSESAAAAYRFTIRPGDTTVFDVEMAIYPRADLDHVGLAPMTSMFFFGPNNRVEVDD
                     FRPSVHDSDGFAIFNGRGEELWRPLNNPKDLQISTFSDLNPRGFGLMQRQKDFREYQD
                     LESNFERRPSLWGEPIGDWGEGSVQLIE"
BASE COUNT          293 a          484 c          509 g          311 t
ORIGIN      
        1 agccctgtca tggtggcagc ggccgttgcc gtagccggcg tgctggcgat ggtggttgtg
       61 gatcatggcc cgtggagccg cccgcacccg gaggcggcca acctggcaaa ccacgggagc
      121 gtggacgagg cggcgcgagc agccggggcg accgtcacgc cgaccgagcc caagtcggac
      181 gtcgagcccg cagtggccgg gccgaagcgc gctcaaccgg tgagtcccgg cgcgccttag
      241 tccgccgctt tgacgtgcta gcggcgacaa gcgctcccca tagggcaagc ggcaatcgcg
      301 gccgtccaaa tagggtgtac tcacgaaacg tgccgccata tccgccataa cccggtacta
      361 ggcgactgaa gctgtgcttc gggagtggaa tcgtcgcggg acgtccagtc gcagctgagg
      421 cggaaggtcg cggtgaaagc ggttccggta cgggaacctg cgaagtcgag cagagtttgt
      481 aggtgttgct ctcgcgtgtt tcggaggtcc cttgaaccgt cgtcgcttcc tgcaagctgc
      541 ggcttcactg ccgctcgcgt cccctctcgc aggcgggatc atctcgccag cgcaagcgga
      601 ctcgtctccg ttcgatcgat cgattgttcg gcagatggcg cgcgacgccg ctgccaagcc
      661 gttcaaggcg ccggaaacga aattgccgga cagcctgaag aagctggact acgatcatta
      721 ccgagccatc cgtttcctgc cggaccgtgc actttggcgt ggcgagaagt tgccgttcga
      781 agtccagttc tttcatcgcg gcttcttcta cgcgcctcgc gtggacatct acgaggttgc
      841 gaacaaccag gcgaccagga tcgtctaccg ccctgagcaa ttctctttcg ggaatacgcc
      901 gccacccagc ccggatgcgg acctgggttt cgccggcttc cggcttcacg ctccgatcaa
      961 ccggcccgac tattacgacg aggtttgcgt cttcctcggc gcgagttatt tccgcgccgt
     1021 cgccaagggc gagctttatg gcctatccgc gcgggggcta gccatcaaca cgggcgaagc
     1081 gaaaggcgaa gaattcccgc agttcaggac gttctggatc gaaaaacctg ccccgaacgc
     1141 caattcggtc gtcgtgcatg cgttgctgga cagtgagagc gcggctgcgg cctatcgttt
     1201 cacgatacgg cctggcgaca cgacggtgtt cgacgttgaa atggccatct atccgcgcgc
     1261 ggatcttgac catgtaggtc tggcgccgat gacgagcatg ttctttttcg gtccgaataa
     1321 tcgggttgaa gtcgacgact ttcgcccgtc cgtgcacgat tcggacggat ttgcgatctt
     1381 caatggtcgg ggcgaggagc tctggcgccc gctcaacaac ccaaaggatt tgcagatcag
     1441 caccttctcc gatctcaatc ctcgcggctt cggcctgatg caaaggcaga aggatttccg
     1501 cgaataccag gatctggaat cgaactttga aaggcgaccc agcctgtggg gtgagccgat
     1561 cggggattgg ggcgaaggct cggttcagct gatcgag
//
LOCUS       DASWQN010000405         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_303117, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000405 DASWQN010000000
VERSION     DASWQN010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303117"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VGC82_06640"
     CDS             complement(<1..849)
                     /locus_tag="VGC82_06640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0422986.1"
                     /translation="MDEFAAGTRTGLAATSDMTNVRWRIFFLILGTVAINYIDRASLS
                     VAMPMISREFAIPPAIQGILLSSFFWTYCFMQIPGGMLADRFLPRSVIAGATIGWGAF
                     AALAAASVGWVTMLLTRLGLGLAEAPIYPAGAKLIGMWMTPTERGRAAALLDGGAPLG
                     TALGSLIIAGLIAAFGSWRLAFLAAGMGTMACGAFAWFYIRNHPHEHESVNPPEREHI
                     ESGLQDDATRDIQVPFARVFRSPSIWMLFVGWFCFNSLWYGFLTWIPSFLAKTQGMNI
                     AALGWAS"
     gene            complement(988..2049)
                     /locus_tag="VGC82_06645"
     CDS             complement(988..2049)
                     /locus_tag="VGC82_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEY0422987.1"
                     /translation="MTRLATAIGAFPLVNPVICASGEPVMTEAGILAALRAGAAGVIA
                     KSVNEHQAAGRQLDRADYVRLDAAGRPTSGPGVSLFNRSGLSQRDTGEWFAAIAAIDR
                     RARHDGRFVAASIVYAGSEGAVSIATQARAAGLRVFELNVGAPHAAEAAPGVIAQETD
                     PDRLAELVRRVRAATEGMQLWVKLTGLSSNLSGLALAARSGGADAVCMMGRFMGLLPD
                     LTTFAPLLGTSAAYGGGWALPIVCRFLAQTRKAAGGFLPLIGTNGVRSGGDVLRMALC
                     GASAVEVLSVTMHEGFGGLTRMIIELDAFLAERGLMFGDLIGRTADGLAGYAEQPEIP
                     GRWREFVPPETLAEAEPPG"
BASE COUNT          443 a          782 c          697 g          347 t
ORIGIN      
        1 cgaggcccag cccaacgccg cgatgttcat cccctgcgtc ttcgccagaa agctcggaat
       61 ccaggtcagg aagccatacc agagcgagtt gaagcagaac cagccgacga acagcatcca
      121 aatcgacggc gagcggaaaa cgcgcgcgaa tggcacctgg atgtcgcgtg tcgcgtcgtc
      181 ctgcaatccg ctctcgatat gctcccgctc cggcgggttg acggactcat gctcgtgggg
      241 atgatttcga atataaaacc acgcgaacgc cccgcatgcc atcgtcccca ttcccgccgc
      301 cagaaacgca agccgccacg acccaaatgc ggcgatcagc ccggcgatga tcagcgaccc
      361 cagcgcggtc cccagtggcg cgcctccatc aagcagcgcc gcggcccggc cgcgttcggt
      421 cggcgtcatc cacatgccga tcagcttggc cccggccggg tagatcgggg cctcggccaa
      481 gccaagacca agccgcgtga gcagcatggt gacccagccg acggaggcgg ccgcaagcgc
      541 cgcgaacgca ccccagccga tggtcgcgcc ggcgattacg gaacgcggca ggaaccggtc
      601 cgccagcatc ccgccaggga tctgcatgaa gcagtaggtc cagaagaaac tgctcagcag
      661 gatgccctgg atcgccggcg ggatcgcgaa ttcacggctg atcatcggca tcgcgaccga
      721 aagggatgcg cgatcgatgt agttgatcgc caccgttccc aggatcagaa agaagatgcg
      781 ccacctgaca ttcgtcatgt cggatgtcgc ggcaagcccg gttcttgttc ccgccgcgaa
      841 ttcgtccatg ccgttcctcc gttttgatca ggcgtcatga tcgggcgtca tgatcgagcg
      901 ccgcgagcgg gaggccagcc tgccgaccgc attcggctac aacggcatga tgacggcggg
      961 gttgccgcgg ctatcagggt cagaaagtca cccgggaggt tccgcttcgg caagcgtttc
     1021 cggcggcacg aattcgcgcc agcggccggg aatctcgggc tgctcggcgt aaccggccaa
     1081 cccgtccgcg gttcgcccga tcaggtcgcc gaacatcaac ccgcgttccg ccagaaaggc
     1141 atccagttcg atgatcatac gggtcaggcc gccgaaacct tcgtgcatgg tcaccgacag
     1201 gacctccacg gcggaggcac cgcaaagcgc catgcgcagg acatcgccgc cggagcgcac
     1261 gccgttggtc ccaatcagcg gcaggaagcc gcccgccgcc ttgcgcgtct gcgccaggaa
     1321 acggcaaacg atcggcaacg cccagccgcc gccataggct gcggacgtgc ccaatagcgg
     1381 cgcgaacgtc gtcaggtcag gcagcagccc catgaacctg cccatcatgc agaccgcatc
     1441 cgcgccgccg ctccgggccg ccagcgccag gccggacagg ttggaggaca ggccagtcag
     1501 cttgacccat agctgcatgc cctcggtggc ggcacgaacc cggcgcacca gttcagccag
     1561 ccggtccgga tcggtttcct gcgcgataac ccccggcgcg gcttccgcgg cgtgcggcgc
     1621 accgacgttc agttcgaaca cgcgcaaccc cgccgcgcgg gcctgggtgg cgatggaaac
     1681 agcaccttcg gacccggcat aaacgatact tgcggcgacg aaacggccgt cgtgccgcgc
     1741 ccgccgatcg atggcggcaa tcgcggcgaa ccattcgccg gtgtcccgct gcgacaggcc
     1801 ggagcggttg aacagcgaca cgcccggccc gcttgtgggc cggcccgccg cgtccaggcg
     1861 gacatagtcg gcgcggtcca actggcgccc cgcggcttga tgctcgttta ccgatttggc
     1921 gattacgccc gcggctccgg cgcgaagtgc cgccaggatt ccagcctcgg tcattacggg
     1981 ttcgcccgat gcgcagatca ccgggttgac cagcgggaag gcgccgatgg cagtcgcgag
     2041 acgggtcatc caggcaaact gctcccacgg cgcccacccg tccagagcgc caccgacttg
     2101 tgttgccaac aagatcttat tgcaccacca caagaatagc cgccgtaatg attgattgcg
     2161 acggaatcac gaagtcatga gttcccgtaa tcgtgaacca atacatgttc tatttctctc
     2221 ggttccgtga attactcgca cacaactcga tatcttctcg ggttcgtga
//
LOCUS       DASWQN010000406         4165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_831286, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000406 DASWQN010000000
VERSION     DASWQN010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831286"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VGC82_06650"
     CDS             <1..1167
                     /locus_tag="VGC82_06650"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY0422988.1"
                     /translation="TVQTRNSEKVTVSVPSWRNDIAAPVTLDQSPALDPAKAQKAAEG
                     CMAIEAENDLIEEVLRLPGLNAVPAVSLPRESPVPATTLTSRQQRTAVARRTLAATGL
                     MECVTFSFMARADAERFGSTRDALRLTNPIAADLDQLRPTPLATLALAAKRNAARGYP
                     DAALFEIGPAFATDAPDGQRLVAAGLRAGHTPRNWQVPQRPLDAMDAKGDLWALLTAV
                     GVSLEGLQTTAGGPSFLHPGRSGTVWQGPKTPMGYFGEIHPRLLAVLDLPAPMVVFEL
                     NLDAVADPKRRKKSAPDLATFQPVRRDFAFIVDIGVPADSVVRAAKGSERTLITGASL
                     FDLYQGDRMAEGKKSLGVEVTFQPRDRTLTDAEIEAACQKVVAAVAKATGATLR"
     gene            1368..1850
                     /gene="cynS"
                     /locus_tag="VGC82_06655"
     CDS             1368..1850
                     /gene="cynS"
                     /locus_tag="VGC82_06655"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEY0422989.1"
                     /translation="MKRAELTEKILDIKRENDWSWKYICEQIGGMSEVLVVGALLGQM
                     KLVKPLAKKAAVLFGLTAVEERMLNEVPHRGTPMPPTDPLIYRFFELVMVNGPAWKAL
                     IEEEFGDGIMSAIDFDMTIDREANPKGDRVKLSMTGKFLPYKYYGNEQGIPEPGFKEK
                     "
     gene            1949..2509
                     /locus_tag="VGC82_06660"
     CDS             1949..2509
                     /locus_tag="VGC82_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0422990.1"
                     /translation="MARVRSIKSAELPPDLAGIYERFAGDYGPFRNQVAVFAHVPAAM
                     RHLMPMLMELREAATLPKRYLEIAIVVVSRLNECDYCVAHHKPFLAVEGISPEGIDRL
                     LDDDNPELDEVDRLVVEYARAAWESPNRMPDTLFRRLRQHFSEAQVVELTLRITLCGF
                     FNRFNDALQIEQEAEAQDRLAALDVR"
     gene            2560..3438
                     /locus_tag="VGC82_06665"
     CDS             2560..3438
                     /locus_tag="VGC82_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0422991.1"
                     /translation="MLQLLASVVLLSSAWPLTKIALAAGSTPLWFAEGRAVLSGLTAA
                     VLLVALGRFHLPTRADLTSVFAIGGLQLGFYLALAHEAVAWVPAGRTAILANTTTIWV
                     VPLSLIFLHEPIPAGRWLAAGLGLAGVTVLINPLAFDWTSGRILIGHIYLLGAGLAWS
                     VAIIVTRAARPGASMFELLPWCFLVGSGVLAPVLWWHAPDGTMGHSPASWWALAYIGL
                     IAGPLGTWCIMEATAKLPTLVSSVGFLTTPAISLILANIFLHEPFTPDLVAGSALIMA
                     GVGFAAWPSGGTTRAA"
     gene            3451..>4165
                     /locus_tag="VGC82_06670"
     CDS             3451..>4165
                     /locus_tag="VGC82_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0422992.1"
                     /translation="MILHAIQAGEGPPVILLHGLFGAARNFGAIQRSLAPRFRVIGLD
                     MRNHGDSPHDPDMRYTEQAADVRETLLALGIGQAAVIGHSMGGKAGMALALTSPDRVG
                     RLLVSDIAPVTYQHGNSAFTGAMRSIPLTPGLTRKAADVALADVVQDQTLRPFLLQNL
                     RFGTQPQWRIGLEEIDAAIPDLEGWADLPGAYSGPTMFVTGARSDYVLPEHRPIIRTL
                     FPTARFVTVKNAGHWVHADN"
BASE COUNT          709 a         1300 c         1393 g          763 t
ORIGIN      
        1 accgtgcaaa cccgcaacag cgagaaagta accgtttccg tgccgtcgtg gcgcaacgac
       61 atcgccgcgc cggtcacgct tgaccagtcc ccggcgctgg atccggcaaa ggcccagaaa
      121 gcggcagagg gctgcatggc gatcgaggcg gagaacgacc tgatcgagga agtcctgcgc
      181 ctgcctggat tgaatgccgt gcccgcggtc tccttgccgc gcgaaagccc ggttccggcg
      241 acgaccttga catcgcgcca gcagcgcact gccgtcgccc gccgcaccct ggccgccacc
      301 ggcctgatgg agtgcgtgac attcagcttc atggctcggg ccgatgcgga acgatttggc
      361 tcgacgcggg atgcactgcg gctgaccaac ccaattgccg ccgacctgga ccaactccgc
      421 ccgactccgc tggcgacatt ggcgctggcg gcgaagcgga atgcggcgcg tggttacccg
      481 gatgcggcat tgttcgagat tgggccggcc ttcgccaccg atgcgccgga cggccagagg
      541 cttgttgctg ccgggctgcg cgccggtcac acgccgcgga actggcaggt gccgcagcgg
      601 ccgttggatg cgatggatgc gaagggcgac ctttgggcgc tgctgaccgc ggtcggcgta
      661 tcgctggaag gattgcagac aacagccgga gggccttcgt tcctgcatcc cggccgctcc
      721 ggcacggtat ggcaggggcc gaaaacgccc atgggctatt tcggcgaaat tcatcctcgg
      781 ctgctggcgg tgctggacct gccggcgccg atggtggtgt tcgagctgaa cctggatgcg
      841 gtcgccgacc caaaacggcg caagaaatcg gcgcccgacc tggcgacttt ccagccggtc
      901 cggcgcgatt ttgcctttat cgtggacatc ggcgtgccgg ccgacagcgt cgtccgggcc
      961 gcgaagggat cggagcggac ccttattaca ggcgcctcgt tgttcgacct gtatcagggc
     1021 gaccgtatgg ccgagggaaa aaagtcgctt ggggtcgagg tgacctttca gccgcgcgat
     1081 cggaccctga ccgacgccga aatcgaggct gcctgccaga aggtggtagc ggcggtggcg
     1141 aaggcgacag gggcgacgct ccggtaagat accgcgctgg ttcgccacgc cgtgccccct
     1201 tcggcctgat cgcggccaat cgtcacgcgc gggttgagtg cccttatcac gggcacccgc
     1261 cggcggcctc gcagccccgg gtttgtggcc cggaattgcg tcttcgggtg cgacgcatgg
     1321 cgcccgctgc gcccgccaaa aacgtccccg ttgtaaagga cagaccgatg aagcgcgctg
     1381 aactcaccga aaaaatcctc gacatcaagc gggagaacga ctggagctgg aagtatatct
     1441 gcgagcaaat cggcggaatg tccgaagttc ttgttgtcgg cgccttgctg ggacagatga
     1501 agctggtaaa gccattggca aagaaggccg ccgtgctgtt cgggctgacc gccgtcgaag
     1561 agcggatgct gaacgaggtt ccgcatcgcg gcacgccgat gccgccgacc gatccgttga
     1621 tctaccgctt cttcgagctg gtcatggtga acgggccagc ctggaaggcg ctgatcgagg
     1681 aggaattcgg cgacgggatc atgtccgcga tcgatttcga catgacgatc gaccgcgagg
     1741 cgaacccgaa gggcgaccgg gtcaagctct cgatgaccgg gaagttcctg ccgtacaaat
     1801 actacggcaa cgaacaaggc attccggaac ccgggttcaa ggagaaatag ctcaggatac
     1861 atcgccggtc ccgcttgcgt gcactaaggg ccgcttggca aagcccaccg agtcatccac
     1921 cgttccgtct gacaggaggg aacatccgat ggcccgcgtt cgcagcatca agtcggctga
     1981 actgccgccg gacctggctg ggatctacga gcggtttgct ggtgactatg gcccgttccg
     2041 caaccaggtc gccgtgttcg cgcatgtacc ggcggcgatg cggcacttga tgccgatgct
     2101 gatggaactg cgggaagccg cgaccttgcc gaagcggtat ctggagatcg ccatcgtcgt
     2161 cgtctcccgg ttgaacgaat gtgactactg cgtggcgcac cacaagccgt tcctggcggt
     2221 ggaaggcatc tcgcccgaag gcatcgaccg cttgctggac gatgataatc cggagttgga
     2281 cgaagtggac cggctggtgg tcgaatacgc gcgggccgcc tgggaatctc cgaaccgtat
     2341 gcccgacacc ttgttcaggc ggctgcgaca gcacttctcc gaagcgcaag tggtggaatt
     2401 gacgttgcgc atcacgttgt gcggtttctt caatcggttc aacgacgcgc tgcaaatcga
     2461 acaggaggcg gaggcacagg acaggctggc agctctggac gtgcgataat cccggcagaa
     2521 gcacagcgca tcaccggaca gccggtgcgg atggcgggct tgctgcaact cctggcgtcg
     2581 gtcgtgctgc tgtccagtgc atggccgttg accaagatcg cactggctgc cggatcgacg
     2641 ccgttgtggt tcgccgaggg tcgggccgtt ctgtccggcc tgacagccgc cgtcctgctg
     2701 gtggcgctgg gccggtttca cctgcccacc cgagccgatc tcacttcggt gtttgctatc
     2761 ggtggactgc agcttggatt ttatctcgcg ttggcgcatg aggctgtggc ctgggtgccg
     2821 gccggccgga ccgcgatcct ggcgaatacc accaccatct gggtcgtgcc cctctcgttg
     2881 attttcctgc acgagccgat acccgccggc aggtggctgg cagccggcct gggactggcc
     2941 ggggtgacgg tgctgatcaa tccgctggcg ttcgactgga catccggcag gatcctgatt
     3001 ggccacatct atctgctggg cgccggcctc gcatggtccg tcgccatcat tgtcacccgg
     3061 gcggcgcggc cgggagcgtc aatgttcgag ctactgcctt ggtgtttcct ggtcggcagc
     3121 ggtgtcctgg cccccgtgct ctggtggcat gcgccggatg ggacgatggg gcactcgccg
     3181 gcctcctggt gggcactggc ctatatcggc ttgatcgccg gcccgctggg aacctggtgc
     3241 atcatggagg ccaccgcgaa attgccgacg ctggtttctt cggtggggtt cctgacgacg
     3301 ccggcgatca gcctgatact ggctaatatc tttctgcacg aacccttcac cccggatctc
     3361 gtggcagggt cggccctgat catggccggt gtcggttttg ccgcatggcc cagcggcgga
     3421 acaacgcggg cagcctgacg gagacagaaa atgatcctgc acgcgatcca ggccggagag
     3481 ggtccgccgg tcatcctgct gcacggcctg ttcggcgcgg cacgcaactt cggcgcgatc
     3541 cagcggtcgc tggcgccgag gttccgggtc atcggtttgg acatgcgcaa tcacggcgac
     3601 agtccgcatg atcccgatat gcgctacacc gaacaggcgg ccgacgtgcg ggagacgctg
     3661 cttgcgctgg ggattgggca agcggccgtg atcgggcact cgatgggcgg caaggccggg
     3721 atggcgctgg cgcttacgtc ccccgatcgt gtggggcggt tgctggtgtc cgatatcgcg
     3781 ccggtgacgt accagcacgg caactcggct ttcaccggcg cgatgcggtc gatcccgctt
     3841 acgccgggcc ttacccgcaa ggctgccgat gtcgccttgg ccgacgtggt tcaggaccag
     3901 accttgcgac cgttcctgtt gcagaacctg cgcttcggga cgcaaccgca atggcgcatc
     3961 ggcctggagg aaatcgacgc cgcgatcccc gacctagagg gatgggcgga cctgccgggt
     4021 gcctattccg gccctactat gttcgtgacc ggcgcccggt ccgattacgt gctgcccgaa
     4081 catcggccga ttatccgaac cctgtttccg acagcccgtt tcgtcacggt caagaatgcc
     4141 ggacactggg ttcacgccga caatc
//
LOCUS       DASWQN010000407         2615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1094572, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000407 DASWQN010000000
VERSION     DASWQN010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094572"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            462..590
                     /locus_tag="VGC82_06675"
     CDS             462..590
                     /locus_tag="VGC82_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0422993.1"
                     /translation="MARGTFKTGQYGLRFLYRHTLGRAWGLFGEKKDCFATAEAAA"
     gene            595..1128
                     /locus_tag="VGC82_06680"
     CDS             595..1128
                     /locus_tag="VGC82_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0422994.1"
                     /translation="MSEDQVRHLLSGIRNPVHKTCLAVMYACGLRISEAITLEVSAID
                     RANQVLRIVGKGNKERLVPLPQPVLDDLSTLWRTHRNRRWLFPNRHGDAPLNKRVLSD
                     TFAAAVTAAGIQRRVTPHCARHSYATRLIENGIDIRIVQIVLGHASIASTAIYTHLTT
                     PTRASLHGLLDRLMRDL"
     gene            1138..2262
                     /locus_tag="VGC82_06685"
     CDS             1138..2262
                     /locus_tag="VGC82_06685"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEY0422995.1"
                     /translation="MIEIADVFRRFAADYVSAHGASLLPSHRRAIEDILDCRTAALGG
                     QVWRCDTCNTEMFSYHSCGNRSCPKCHTAQTQEWLERRRAEMLPVPYFHITITVPAEL
                     REMLRANQRDGYGVLMQASAAAIIELARDPRYVGGTVGVLAVLHTWTQQLAFHPHVHC
                     LVSGGGISEDGNTWHPARRNFLVPIKALAKLVRGKFRALLRQKCLDLVMPEAVWDRPW
                     ILHVTAWGNGEQAVLDYLARYVFRIALTHARIVGLDDETVTLQYRHRKTGCARTCRLR
                     GEEFMRRFLQHVLPRGFHKVRYFGLWHPAHRDDATRVRQMLQLQAQPKLDPAQDPEVP
                     SLVPADAEPVRLVKPMICPHCHQGRLIFIRRLTRQQAMGP"
BASE COUNT          471 a          877 c          771 g          496 t
ORIGIN      
        1 acccagcaca tctccgtggt cgtcgcgtcc catctctgac gatccaaccg gcagagcaga
       61 gggccacccc ggggcgttgt tgcatatgtg gatggggtta cgagggactc cctgcaacct
      121 cgtacgttaa gggtctacgc gttttggatg acgggaatta cctgttggac ggaacggctt
      181 cgccgaggcc ccccgggtag cgaggtcaat ggctagttgg acgtatcctt tcgatcggcg
      241 ggcatgaggt tgcccgccgg aactgcaacg aaaggagacc accatgtcgc cactacgtat
      301 gcggatgatc gaggacatga ccctggctgg attggctgag ggaacgcaga agatctacat
      361 ccaggcggtg cgccggctag ccgcgcatta ccgtcgctcg cccgatgagc tcagcgagga
      421 agagggtatg gggctacctg ctcgacctac ggcagcgggg tgtggcacgc ggcaccttca
      481 agaccggtca gtacggcctg cgttttctct accgccatac gctcggccgg gcctggggcc
      541 tgttcgggga aaaaaaggat tgcttcgcca cggcagaagc ggctgcctga cgcactgtcg
      601 gaagatcagg tccgtcatct cctcagcggt atccgcaacc cggtccacaa gacgtgcctt
      661 gccgtcatgt atgcgtgcgg tctgcgcatc agcgaggcca ttacgctgga agtcagcgcc
      721 attgaccggg ccaaccaggt gctgcgcatc gtcggcaagg gcaacaagga gcgactggtg
      781 ccgctgccac agccggttct cgatgatctc agcaccttgt ggcgcaccca tcgcaaccgc
      841 cgctggctat tccccaaccg acatggggat gcgccgctca acaagcgcgt gctgtcggac
      901 accttcgccg ccgctgtcac cgcggccggc atccagcgga gggtgacgcc gcattgtgcg
      961 cgccactctt acgcgacgcg gctgatcgag aacggtatcg acatccgcat cgtacaaatc
     1021 gtgctcggcc acgccagcat cgcctccacc gccatttaca cccacctgac cacgccgacc
     1081 cgggcgtcgc tgcatggtct gctcgatcgg ttgatgcgcg acctctgagg cgcggtcatg
     1141 atcgagatcg ccgacgtgtt ccgtcgcttc gcggcggact acgtttcggc tcacggcgcg
     1201 tcgctgctgc cctcgcatcg gcgtgccatc gaggacattc tcgattgtcg caccgccgcc
     1261 ctcggcggcc aggtctggcg ctgcgacacc tgcaacaccg agatgttctc ctatcattcc
     1321 tgtggcaacc gcagttgccc gaagtgccat accgcacaga cccaggaatg gctcgaacgc
     1381 cggcgggcgg agatgctgcc agtgccttac ttccacatca ccatcaccgt gccggccgaa
     1441 ctacgtgaga tgctgcgcgc caaccagcgt gatggttatg gcgtgttgat gcaggccagt
     1501 gccgccgcga ttatcgaact cgcccgtgat ccgcgttacg tcggcggcac ggtcggcgtg
     1561 ctggctgtgc tgcatacctg gacacaacaa ctggccttcc acccgcatgt tcattgcctg
     1621 gtcagcggtg gcgggatctc cgaagacggc aacacctggc atccggcgcg tcggaatttc
     1681 cttgtcccga tcaaggcgct tgccaaactg gtgcgaggca agttccgcgc gttactgcgg
     1741 caaaaatgcc ttgatctcgt catgcccgaa gccgtatggg acagaccctg gatcctccat
     1801 gtcaccgcct ggggcaacgg cgagcaggcg gtgctcgact atctcgcccg ctacgtcttt
     1861 cgcatcgctc tgacccatgc ccgcatcgtc ggtctcgacg acgaaacggt caccctccaa
     1921 tacagacatc gcaagaccgg ctgtgcccga acctgccgtc tgcgcggcga ggaattcatg
     1981 cgccgcttcc ttcagcatgt gctgccgcgc ggctttcaca aggttcgcta cttcggcctc
     2041 tggcatcccg cgcaccgtga tgacgccaca cgggtccgcc aaatgttgca actccaggcg
     2101 caaccgaagc ttgatccggc gcaggatccc gaagtcccat cgctcgtgcc ggctgacgca
     2161 gaaccggtgc ggttagtcaa gccgatgatc tgcccgcact gtcaccaggg gcggttgatc
     2221 ttcatccgca ggctcacccg acagcaggcc atggggccat gatccgcacc gtctgccttc
     2281 gccgctgcct gaacttgctg ctgcgcgcct cggcacgcca caggggtccg ctgtcgccat
     2341 cataccccat gcgccgaact ttgccccaga tgatgccgct gatcagccaa atagtatcgc
     2401 gttggaccac gatatccagg tccagctcct cgcaccgtgg ctcctgaacg gtgatcccgg
     2461 gctccggcat caagcgcacc ggttgaaatc tccatagcgc tccatcgccg ctcaacgccc
     2521 cacgttcgtc caacggggct tctgcggcgg ctacgcgccg cacaaaccct taattcgttt
     2581 ccggttgatg ccggaaagga tcctgagact gtgcg
//
LOCUS       DASWQN010000408         4955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_567242, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000408 DASWQN010000000
VERSION     DASWQN010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567242"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..751)
                     /locus_tag="VGC82_06690"
     CDS             complement(167..751)
                     /locus_tag="VGC82_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438965.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HEY0422996.1"
                     /translation="MDWTQDLLTLAQVVMIDVALAGDNAVVVGLAVAGLPSRQKRRAI
                     LLGIGGATVIRIAMGAVALQLLAVIGLLLAGGALLLWVCWKMFRELRQPHRVATGGAA
                     TKTLRQAMMQIVLADVSMSLDNVLAVAGAAHDRFWVLATGLVLSVGLMGLAAGLIARW
                     LEQYRWIAWAGLLIVLYVALNMIWHGGQEVAEAM"
     gene            814..2145
                     /gene="purB"
                     /locus_tag="VGC82_06695"
     CDS             814..2145
                     /gene="purB"
                     /locus_tag="VGC82_06695"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015074094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY0422997.1"
                     /translation="MIPRYSRPEMAAIWAPENRYRIWFEVEALAAEGMARIGAIPEEA
                     ARNIREKGAPKLAAMNAADVERIDAIERETRHDVIAFLTWLAEAVGPDSRFVHQGMTS
                     SDVLDTTLSVQLTQAADLLLAGIDAVMAALKARAIEHKYTLTIGRSHGIHAEPTTLGL
                     KLAGHYAEFARNRERLVAARAEVAVAAISGAVGTFAHLDPSIEEYVAEKLGLRAEAVS
                     TQVIPRDRHAAFFCTLGIIASGIERLATEIRHLQRSEVREAEEFFHPGQKGSSAMPHK
                     RNPVLSENLCGLARIVRSAVVPALENVTLWHERDISHSSVERAIGPDATVTLDFALMR
                     LAGMMEKLLVYPQRMLANLESLGGVVHSGEVLLALTKAGISREDAYRIVQNCAMATWT
                     KLGTPEGRSFRDNLQADPDVARIVKPAELDAAMDPGLHLRSVDRIFARVFG"
     gene            complement(2280..2885)
                     /gene="raiA"
                     /locus_tag="VGC82_06700"
     CDS             complement(2280..2885)
                     /gene="raiA"
                     /locus_tag="VGC82_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY0422998.1"
                     /translation="MQITVAGKQVDLSDALRTRVSTQMDTIANKYFDHALEAHVTFSR
                     ARSFFTCDINVLAGRGLKLRGEGEAADANTAFDDAAEHIAKRLRRYRRRVNDHHRDMA
                     DRDQPETAQQYILREEANGADPEIADGRTGDETFATVVAETQTEISVLSVSEAVMRMD
                     LADQSVMMFRNSASGVLNVVYRRSDGHIGWIDPGSPLTPDA"
     gene            complement(3089..4591)
                     /gene="rpoN"
                     /locus_tag="VGC82_06705"
     CDS             complement(3089..4591)
                     /gene="rpoN"
                     /locus_tag="VGC82_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY0422999.1"
                     /translation="MAIAPRLDLRQSQTLVMTPQLRQAIKLLQFSNIEVNSFVEEEME
                     RNPLLERDESPEPPVGERAALDQIEPRTSLVVDSADAARSDTLPADAGSPLDTAHAEM
                     YDPGGAGDGTPLVGRMDGSGGSHSFDADGRGIDDYAGNDRSLRDHLGEQLRLNFTDPV
                     DRMIGAHLIALLCPAGRLTAEPVAIAQAMALPLERIELVRARMMRFDPVGLFARNLRE
                     CLAAQLAERNRLDPAMQMLLDNLDLLAKRENRRLMTLCGVDAEDLTDMIAEIRALDPK
                     PATSWDTTAPQLVVPDLLMRRLPDETWIIELNPETMPRVLVNERFYAKVTAGAKKDEK
                     VFLTERLANANWLVRSLQKRAQTILKVAAEILRQQDGFLQLGVAYLRPLILRDIAEAV
                     ELHESTVSRVTANKYIATPRGTFELKYFFTTAIHGTDGRDSHSAEAVRHRIRGMIDTE
                     AVPDVLSDDAIVVMLRREGVEIARRTVAKYREALRIPNSVQRKRDKTVPA"
     gene            complement(4698..>4955)
                     /locus_tag="VGC82_06710"
     CDS             complement(4698..>4955)
                     /locus_tag="VGC82_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008854022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY0423000.1"
                     /translation="SYILLDEPLAGIDPIAVNEIRDLVKHLKDRGIGVLITDHNVRET
                     LDIIDRAYIMHDGQLLMEGKPSEVVAHEDVRRVYLGEKFSL"
BASE COUNT          926 a         1649 c         1479 g          901 t
ORIGIN      
        1 cgcatgatcg tccgtcgccg cgcagcggtt tggtgtcgtg ccactgaagt ccgttacact
       61 gtgtcaagca tccctctcgg ttcgctcaag caaacccgtt tcggcgacag ccgacgttca
      121 ggcaaaacag gcaaaacagg caatacgggc aaccgggcaa aacaggctac atcgcctccg
      181 ccacctcctg cccgccgtgc cagatcatgt tcaacgcgac atacagcacg atcagcagcc
      241 cggcccaggc gatccaccgg tactgctcca accaccgcgc gatcaacccg gccgccaggc
      301 ccatcaggcc cacggaaagc accaggccgg tggccagcac ccagaaccgg tcgtgcgcgg
      361 caccggcaac ggccagaacg ttatccaggc tcatggacac gtcggccaat acgatctgca
      421 tcatcgcttg acgcagcgtt ttcgtagccg cgcccccggt ggcgacccgg tgcggctggc
      481 gcagctcccg gaacatcttc caacaaaccc atagcagcaa ggctccgccg gccagcagca
      541 gcccgatgac ggcaagcaac tgcagcgcca ctgcgcccat cgcaatgcgg atcaccgtgg
      601 caccgccgat ccccagcagg atcgcccgcc gcttctgccg cgacggcaat ccggccacgg
      661 ccaggccgac caccacggcg ttgtctccgg ccagtgccac gtcgatcatc acgacctggg
      721 ccaacgtcag caggtcctgt gtccagtcca tgcttgcctt ccgcgatgcc tcggttttag
      781 ctgggaatct tcctcgtatt ggacaaggtg tcgatgatcc cgcgttactc ccgtcccgaa
      841 atggcagcca tctgggcgcc cgagaaccgc taccggatct ggttcgaggt cgaggcgctc
      901 gccgccgaag gcatggcccg catcggcgcc atcccggagg aagcggcccg caacatccgg
      961 gagaagggtg ccccgaagct cgccgcgatg aacgccgcgg atgtggagcg gatcgacgcg
     1021 atcgagcggg aaacccgcca cgacgtcatc gccttcctga cctggctggc cgaggcggtc
     1081 ggcccggact cgcgcttcgt gcatcagggc atgaccagtt ccgacgtgct ggacacgacc
     1141 ctgtcggtgc aactgaccca ggcggccgac ctgctgttgg ccggcatcga cgcggtgatg
     1201 gccgcgctga aggctcgcgc gatcgagcac aaatacacgc tcaccattgg ccgcagccac
     1261 ggcatccatg ccgaacccac gacactcggg ctgaaactgg ctggccacta cgccgaattc
     1321 gcccgcaacc gcgagcgtct ggtcgccgct cgggctgaag tggcggttgc cgcgatcagc
     1381 ggagcggtgg gtaccttcgc ccatctggac ccgagcatcg aggagtatgt cgcggagaaa
     1441 cttgggttgc gggcggaagc tgtctcaact caggttattc cgcgcgaccg acacgcggcg
     1501 ttcttctgca ctttgggcat catcgccagc ggtatcgagc gtttggcgac ggaaatccgg
     1561 catctgcaac ggtcggaggt gcgggaggcg gaggagttct tccatcccgg ccagaagggc
     1621 tcctcggcga tgccccacaa acgaaatccg gtgttgagcg agaacctatg cggcctggcc
     1681 cgaatcgtcc ggtccgcagt tgtgccggcg ctggagaatg tgacgctgtg gcatgagcgc
     1741 gacatcagcc attcatcggt agaacgggcg atcggcccgg acgcgacggt gacgctggat
     1801 ttcgcgctga tgcggctggc cgggatgatg gagaagctgc tggtgtatcc ccagcggatg
     1861 ctggcgaacc tggaaagcct ggggggcgtg gtgcattccg gcgaggtgct gctggcgctg
     1921 accaaggcgg ggatctcgcg cgaggacgcc tatcgcatcg tccaaaactg cgccatggcg
     1981 acatggacca agcttggcac gccggaaggg cgatcatttc gggataacct gcaagccgat
     2041 ccggatgttg cccggatagt caagccggcg gaactagacg cagcgatgga cccaggcttg
     2101 cacctgcggt cggtggaccg gattttcgcg cgggtgtttg gttaggccca gcgtagcgag
     2161 agagctgcca aacggctaca tccgatcgac agccggcgtc ataaactccg ctccggacgg
     2221 ccaattaaac ggtcggactg gcagcacctc gcggctgacg ccgggccgcg aaagcttgct
     2281 caggcatcag gtgttaacgg cgatcccggg tcgatccagc cgatatgccc gtcgcttcgc
     2341 cggtatacga cattcaacac gccggatgcg ctgttgcgaa acatcattac cgactggtcc
     2401 gcaaggtcca tccgcatcac cgcctcgctc accgacagaa cggaaatctc ggtctgagtc
     2461 tccgccacca cggtcgcgaa agtctcatcc ccagttcgcc catcggcgat ttcagggtcc
     2521 gccccgttcg cctcctccct caggatatac tgctgcgcgg tctcgggctg gtcacgatcc
     2581 gccatgtccc gatgatggtc gtttacccgc cggcgatagc ggcgaaggcg tttggctatg
     2641 tgctccgcgg catcgtcgaa ggccgtgttg gcatccgctg cctcgccttc gccccgcaac
     2701 ttcaatcccc ggccggcaag aacattaata tcgcatgtga aaaaacttcg tgcccggctg
     2761 aacgtcacgt gcgcttccaa cgcatgatcg aagtatttat tggcgatcgt gtccatctgg
     2821 gtggacaccc gcgtccgcaa cgcgtccgac aagtcgactt gtttgccggc aaccgtgatt
     2881 tgcatcgtaa tctcccgtcc agttcgacag gccgcccggg cagttccatg gccggtgtcc
     2941 cggcggctac ggcatttggc cacgccgtcg acggcaatgt ccactacgga aaagcgcagc
     3001 gtggctgagc acagttcgat cgctgaccat acgatgacta atatttcatt cctgcgggcc
     3061 tgcgcgatcc gcgtcggctg ccgcaggctt acgctggcac cgtcttgtcg cgcttgcgct
     3121 ggaccgagtt cggaatccgc agcgcctccc gatacttcgc caccgtccgg cgggctatct
     3181 cgacaccctc ccggcgcaac atgacaacaa ttgcatcgtc ggacagaacg tccggtacag
     3241 cctcggtgtc aatcattccg cgaatgcggt gccttaccgc ctcggcgcta tgcgagtcgc
     3301 ggccatccgt gccgtggatc gcggtggtaa agaagtattt cagctcgaac gtgccgcgcg
     3361 gcgtggcgat atacttattc gcggtcactc ggctgacggt gctctcgtgc agctccaccg
     3421 cttcggcgat atcgcgcaga ataagcggtc gcagataggc gacgccgagc tgcaggaatc
     3481 cgtcctgctg gcgcagaatc tcggccgcga ccttcaggat ggtctgcgcc cgcttctgca
     3541 aggaccgcac cagccagttc gcgttcgcca gccgctcggt cagaaagacc ttctcatcct
     3601 tcttcgcccc ggccgtcacc ttggcataga accgctcgtt caccagcacc cgaggcatcg
     3661 tctcggggtt caactcgata atccaggttt cgtccggcaa ccgccgcatc agcagatccg
     3721 gcacgacaag ctgcggcgcg gtggtatccc agctcgtcgc gggcttcgga tcgagcgccc
     3781 ggatctcggc gatcatgtcc gtcaggtctt cggcatcgac gccgcacagc gtcatcagcc
     3841 gccggttttc ccgctttgcc agcaggtcga gattgtccag cagcatctgc atcgccggat
     3901 ccagccggtt gcgctccgcc aattgcgctg cgaggcactc tcgcaagttc cgcgcgaaca
     3961 atcccaccgg atcgaaccgc atcatgcggg cgcgaaccaa ctcgatccgc tccaacggca
     4021 gcgccatcgc ttgggcgatc gctaccggtt cagccgtcaa ccggccggcg gggcagagca
     4081 gcgcgatcag gtgcgcccca atcatccgat ccaccggatc ggtgaagttc agccgcaact
     4141 gctcgcccag atgatcccgc agcgaccggt cgttaccggc ataatcatcg atgccgcggc
     4201 catcggcgtc gaaactgtgg ctgccaccgc tgccgtccat gcgtccgacc agtggcgtgc
     4261 cgtcacccgc tccgccggga tcgtacatct cggcatgcgc cgtatccaaa ggcgagccgg
     4321 cgtcggccgg taaggtatcc gatcgcgccg catcggccga atccaccacc agtgacgtac
     4381 gcggttcgat ctggtccagc gccgcccgct cgccaaccgg cggttcgggg gattcgtcgc
     4441 gttcgagaag gggattccgc tccatctcct cctcgacgaa ggagttgacc tcgatattgg
     4501 aaaactggag cagcttgatg gcctggcgca gctgcggcgt catgaccagc gtctggcttt
     4561 ggcgtaggtc gagacgggga gcaatcgcca tcaggcaatt atcaagcgat acggccccag
     4621 gtcaaatgga ttccgtcatg attatgcaaa aagcatgcaa ctgcgcccgt tgccgcgaat
     4681 ttaagaggcg gcagttctca taaactgaac ttttccccca aatatacccg ccgcacatct
     4741 tcgtgagcaa ccacctcgct tggtttgccc tccatcagaa gctgaccatc gtgcatgata
     4801 tatgcgcgat cgatgatatc gagtgtctcc cgcacgttgt ggtcggtgat cagcacgccg
     4861 atgccgcggt ccttcaggtg tttcacgagg tcgcggatct cattcaccgc gatcgggtcg
     4921 attccggcca acggctcgtc cagcaggata tagga
//
LOCUS       DASWQN010000409         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1094615, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000409 DASWQN010000000
VERSION     DASWQN010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094615"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..546)
                     /locus_tag="VGC82_06715"
     CDS             complement(202..546)
                     /locus_tag="VGC82_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423001.1"
                     /translation="MTDLLRGAVPAGQTERGPEKIVMLLADPVIERIAAYPWPRGGVS
                     VRKRRGGYSLSSARTGAPVARLKPKPQHHRFEVLWWRRDAWGSAGPFGAVFSLDDALD
                     FIASEPPFWIRA"
     gene            545..1423
                     /locus_tag="VGC82_06720"
     CDS             545..1423
                     /locus_tag="VGC82_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0423002.1"
                     /translation="MVRPDLHLPQAFVAWMAPFLAAFCRRSRDTAAALTVGALLAIGP
                     RTVTNCLRALGLAEHPGFTAFHRVLNRNVWSGLALGRTLLRLLVPAFEPGRPFVIIGV
                     DHTLERRRGRRIEPASHFHDAVRSTGKQKVTSRGLRWISAMLLVQVPFAARIWGLPVL
                     TALTPSKAWCEHHKRRYRPVTKWAELLLLTLHRWLPERVIVAVMDGEFAAIDLLHRLR
                     RCMVVITRLRLDACLFDPPSAYKGRGRYPKKGKRQPSLKARITDSGTVWKRAVQTSRT
                     SWRSGGWIEHASGTAL"
     gene            1523..1903
                     /locus_tag="VGC82_06725"
     CDS             1523..1903
                     /locus_tag="VGC82_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423003.1"
                     /translation="MALLDVLDCYNRRWSMETTYEEARAHLGVETQRQWSDPAIFRTT
                     PLLFGLYSLITLYIQQNAERLALSPRRAAWYPKPAATFADALARLRQHFWFERFATSA
                     EAPDMKQFLPPDLQRLIQLACYAP"
BASE COUNT          367 a          730 c          653 g          387 t
ORIGIN      
        1 gtccgaggtc cgcgccggag tgccaaacgc ccttggatgc ggccaagctt ggtgacgcag
       61 tgacgcaccg tgacgcactt tctaatggag gtagcaatat gcgcgcgcac aatagcactc
      121 ccatagaagt gggtcacaat gcgtcatgtg cgtcatgtgc gtcatgtgcg tcctggactt
      181 tgcccattcg cgaaacggtg atcaggctcg gatccagaaa ggcggttccg aggcgatgaa
      241 gtcgagcgcg tcgtcgagcg agaagacggc gccgaaggga ccggctgaac cccaggcgtc
      301 gcgacgccac cacagcacct cgaagcggtg gtgctgcggc ttcggcttca gcctggcaac
      361 gggggcgccg gtgcgggcgg atgacagact atagccgccg cggcgcttgc ggacgctgac
      421 gccgccgcgc ggccagggat aggccgcgat ccgctcaatc acaggatccg ccagcaacat
      481 aacgatcttc tccggccccc gctccgtttg gcctgcaggc accgcccccc gcaggaggtc
      541 tgtcatggtt cgcccggatc tgcatcttcc gcaagccttc gttgcctgga tggcgccctt
      601 tctcgccgcc ttctgccgac gcagccgcga caccgctgcg gccctgacag tcggcgccct
      661 gctggcgatc gggccgcgca ccgtcaccaa ttgcctgcgc gctctcggtc tggccgagca
      721 tcctggcttt accgcctttc accgcgtgct caaccgcaat gtctggtcgg gcctggcgct
      781 gggccgaacc ctgctgcgat tgctggtccc tgcctttgag ccgggccggc ccttcgtcat
      841 cattggcgtc gatcataccc tggaacgccg ccgcggtcgc cgcatcgagc cggccagcca
      901 cttccacgac gccgtccgct ccaccggcaa acaaaaagtc accagtcggg gtctgcgttg
      961 gatcagcgcg atgctgttgg tccaagtgcc gtttgccgct cgcatctggg gactgccggt
     1021 gctgaccgca ctgacgccgt ccaaggcctg gtgcgagcat cacaagcggc gctatcgccc
     1081 ggtgaccaaa tgggccgaac ttctgctact gacgttgcac cgctggctgc cggagcgcgt
     1141 gatcgtggcg gtcatggacg gcgaattcgc cgccatcgac ctgctgcacc ggctccgtcg
     1201 atgcatggtc gtcatcaccc gcctgcggct ggatgcctgc ctgttcgatc ctccctcggc
     1261 ctacaaggga cgcggccggt atcccaagaa aggtaagcgg caaccgtcgc tgaaggcgcg
     1321 catcaccgac tcgggcacgg tttggaagcg ggcggtgcag accagccgca catcctggcg
     1381 cagcggcggc tggatcgagc acgcctccgg aacggcgctg tgataccacg ctggcaagga
     1441 gccgctgccg atccgctgga tactggttcg ttacccggac cagcggcatg ataccgaggc
     1501 ttttctgtgc accgatacgc aaatggccct gcttgacgtg ctcgactgct acaatcgccg
     1561 ctggtcgatg gaaacaactt acgaagaagc acgcgcccat ctcggcgttg aaacacagcg
     1621 ccagtggtcg gacccggcga tctttcgcac cacaccgctg ctgttcggcc tgtattcgtt
     1681 gatcaccctc tatattcagc agaacgccga gcggctggcg ctgtcgccac gccgggccgc
     1741 ctggtatccc aagccggcgg ccaccttcgc cgatgccctc gctcgtctgc gccagcattt
     1801 ctggttcgag cgtttcgcca cctcggccga ggcccccgac atgaagcaat tcctcccgcc
     1861 ggatttgcaa cggctaattc aactcgcgtg ctacgcgccc tgacctgaca gttcagccga
     1921 aagtcgcagc gaatgaggca tctcaagcgg ggcctctgag ggaaaatcag actcagaacc
     1981 gatccctgaa ttccgaactc ggcctctcgg gcgccggaat gggcaaagtc cagtccatcg
     2041 ttaggttgga ttgcaagaac gatggaggtc cgacccacgt ccggccgcgc tcccggcagc
     2101 cgccgtcggc gtgctgtccg aatttcgacc tacgccg
//
LOCUS       DASWQN010000410         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1490568, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000410 DASWQN010000000
VERSION     DASWQN010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490568"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VGC82_06730"
     CDS             complement(<1..520)
                     /locus_tag="VGC82_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0423004.1"
                     /translation="MRTWYFSEMAYHPAWEKGLARGSLRVNFPSENLDPTDAGGLLNR
                     YLDEFALCDEVGLDIMVNEHHSTATCMTVSVPMALSIIARETTKARLLSLGNPIANRP
                     DPVRVAEEMAWLDCLSGGRIEMGLVKGAPYEIAPANSNPGRLMRRYWEAHDLIIKALS
                     TTTGPFSWEGEFY"
     gene            complement(595..1380)
                     /locus_tag="VGC82_06735"
     CDS             complement(595..1380)
                     /locus_tag="VGC82_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0423005.1"
                     /translation="MSRHVTVSGVKLEIEEKGQGRPILFLHSGEGLQPNRPWLDALAQ
                     HHRVIAPHHPGFGGSALPDWVGTVDDLAYLYLDLAEHLRLESAVLAGNCFGGWVAAEM
                     AVRNTTRFAGLLLVDPLGIKVGGVLDRDITDMHAISRSEFLRLAWADAANGETDYTKL
                     PDTELAAIARGREALVVFGWKPYMHNPRLKRWLHRIDIPTHLLWGEQDGIVSVAYGEA
                     WKAEIPGATMETIPNAGHYPQWEQPDGFASRVHAFTNRLGPTL"
     gene            2023..>2987
                     /locus_tag="VGC82_06740"
     CDS             2023..>2987
                     /locus_tag="VGC82_06740"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEY0423006.1"
                     /translation="MANQDDIALMAHLMRRASFGASRDELEARVAKGYEATVEELLNP
                     ESQPPVDPYTLLRYQPAALLPGGQPPMGNVNYMYYLVTTKRPLQEKMALFWHHVFATG
                     NSKVDNYDQLLEQIEIFRNNSMGNYRDLLLKMAKNPTMIFWLDNNQNHGSAVNENWGR
                     ELLELFSLGAGNYTETDVREASRAFTGWTFETKIPRLPYGRFPWKFEYRPEDHDDGMK
                     EFLGVRGHLNGEDIIDIIAQQPACAKFICRHLYNFFVADEPQVPAWSLEPPRDPEALE
                     AMAKCLRDSNYEIRPVLRMMFNSDWFKAARYQHLKSPAEVVVGTLR"
BASE COUNT          578 a          898 c          958 g          553 t
ORIGIN      
        1 ggtagaactc accctcccag gagaatgggc cggtcgttgt cgacagcgcc ttgatgatca
       61 gatcgtgggc ctcccagtat cgccgcatca ggcggccggg gttggagttg gcgggggcga
      121 tttcgtaggg ggcgcctttt accaggccca tttctatgcg gccgccggac aggcaatcga
      181 gccaggccat ttcctcggcg acccggacgg gatcggggcg gttggctatg gggttgccca
      241 ggctgagcaa gcgcgccttg gtggtttccc gggcgatgat cgacagcgcc atcggcaccg
      301 aaacggtcat gcaggtggcg gtgctgtggt gttcgttcac catgatgtcc agcccgacct
      361 cgtcgcataa ggcgaattcg tcgaggtagc ggttcagcag gccgccggcg tctgtcggat
      421 caaggttctc actggggaaa ttgacccgca gcgagcctcg ggccaagccc ttctcccacg
      481 cggggtgata agccatttcg gaaaagtacc aggtgcgcat ttcgttgctc ctagggcatc
      541 gtcacgctcg gtgggatgct ccgaaccgag cgaggcgtgc ggtcaaaata cggatcagag
      601 cgtcggcccg aggcggttgg taaacgcgtg gacgcgcgac gcgaaaccgt ctggctgttc
      661 ccactgcgga taatggccgg cgttcgggat cgtttccatc gtggcgcccg ggatttcggc
      721 cttccacgct tcgccatagg cgacggagac gatgccgtcc tgctcacccc acaacaggtg
      781 tgtcgggatg tcgatgcggt gcagccagcg tttcaggcgc ggattgtgca tgtagggctt
      841 ccagccgaat accacgagag cctcgcgacc gcgggcgatg gcggcgagtt cggtgtccgg
      901 cagtttggta tagtcggttt cgccgttcgc cgcgtcggcc caggcgaggc ggaggaactc
      961 ggaccgggaa atggcgtgca tgtcggtgat gtcgcggtcc agcacgccgc caaccttgat
     1021 gccaagcggg tcaaccagca gcaggccggc gaagcgggtg gtgttgcgga ccgccatttc
     1081 ggccgccacc cagccgccga agcagttgcc cgccagcacg gcgctctcca gtcgcaggtg
     1141 ttcggccaga tcgaggtaaa ggtaagcaag atcatcgacg gtaccgaccc agtcgggcaa
     1201 tgccgaacca ccgaagccgg gatgatgggg cgcgatgacg cggtggtgtt gcgccagcgc
     1261 gtccaaccag ggacggttgg gttgcagacc ctcgccggaa tgcaggaaca ggatcggacg
     1321 cccctgcccc ttctcctcga tttccagctt gacgccggag acggtgacgt ggcggctcat
     1381 gccggattgc cctttctggt gtcgctggat ggagcttacc caaccaggtg cgacgggcaa
     1441 cctttgccca cgatgcatcc aggccaagcc ccggtcgtcg ccgcttgctg gatggttgtg
     1501 ccgcaagctg ttcaaaattc cgtgtgaatc ctggtccggg gccgccgttc gatgcttcta
     1561 tatcgatggc gggcacgatg agccggctag tcgtgaagcc tcgctcttga cggatggcac
     1621 ggcctgcgac accgtccgcg tgtggccacg cgtcttcagc gaatggggaa acggcgaagt
     1681 ggtgctctgg aaactcgtga gcgaggcagc ttgttggagc gcaggatacg gtttttcctg
     1741 cggccggcga tgatggcgcc ttcgagacga agtcatacag gcaggatcgg ctctgcccag
     1801 cttgaacgcc ggcgcatttc ccttgctgcg gccgcgtcga tgctatcagc ctgctatgcc
     1861 ggccagcctg ttaaagtttc tgccgacagc atggcgatcg ataactggcc cttcgcggcc
     1921 gcgtcgcgtt gtgccggctg acacaggacc ggcacatgac gagcaacgcc ggcgcatgaa
     1981 tagaatcgat aaagaatcat gaatgacaaa gggagactaa gcatggcaaa ccaagacgac
     2041 atcgccctga tggcccattt gatgcgccgc gccagcttcg gagcatcgcg tgacgagctg
     2101 gaggctcggg tggccaaagg ctacgaggcg acagtcgagg aactgctgaa ccctgaaagc
     2161 cagcctccgg tcgatcctta tacattgctg cgctaccagc cggcggcact gttaccgggc
     2221 ggccagcccc cgatgggcaa cgtcaactac atgtactacc tggtgaccac caagcgcccg
     2281 ctccaggaga aaatggcgct gttctggcac catgtgttcg ccaccggcaa ctccaaggtc
     2341 gataactacg atcaattgct ggagcagatc gagatcttcc gcaacaacag catgggcaac
     2401 tatcgcgacc tgctgttgaa gatggccaag aacccgacga tgatcttctg gctggacaac
     2461 aaccagaacc atggatcggc ggtcaacgag aactggggcc gtgagctgct ggaactgttc
     2521 agcctcggcg ccggcaacta taccgaaacg gacgtacgcg aggcctcgcg cgccttcacc
     2581 ggatggacct tcgaaacaaa gatcccgcgc ctgccctacg gccggttccc gtggaagttc
     2641 gaataccgtc cggaagacca cgacgacggc atgaaggaat tcctcggcgt tcgcggccac
     2701 ctgaatggcg aagacattat cgacatcatc gcccagcagc cggcctgcgc caaattcatc
     2761 tgccggcatc tgtataattt cttcgtcgcc gacgaacccc aggtacctgc ctggtcgctg
     2821 gagccgccgc gcgatccgga agcgctggaa gccatggcga agtgcctccg cgattcgaac
     2881 tatgaaatcc ggcccgtgct acggatgatg ttcaactccg actggttcaa ggcggctcgg
     2941 taccagcatc tgaagtcgcc agccgaagtg gtcgtgggaa ccttgcg
//
LOCUS       DASWQN010000411         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_303769, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000411 DASWQN010000000
VERSION     DASWQN010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303769"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VGC82_06745"
     CDS             <1..411
                     /locus_tag="VGC82_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423007.1"
                     /translation="MELIASRRLPLVLGLLASSAFTGLAATATSAGAAVTFIALSMFC
                     MQFGITCKWILVTAVTPQAYCASAASNQNFAGFLGGTLSPILTGFIVDMTGSFVLALA
                     IGAVITLIGAALFQFMMTTVIDEGELEKYAHAPA"
     gene            611..1489
                     /locus_tag="VGC82_06750"
     CDS             611..1489
                     /locus_tag="VGC82_06750"
                     /inference="COORDINATES: protein motif:HMM:NF033520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEY0423008.1"
                     /translation="MFDQLVERFCEFDDFCQAVRPQWEATLLTEGKAGRRKPGPERGL
                     ADSEVMTLLVLYHSSRFKHIKAFYNGVVLTILRPYFPDAPCYERFLSITSSVWPLLVF
                     FLVSRMGARTGFYYIDSTPLAVCHNRRISRHKVFAGLAERGKTSIGWFFGFKLHLVFN
                     HQREIVALKLTPGNVSDTGPVPELTQGLIGKLFGDKGYIGRELAQELLRRGLALMTRV
                     RKNMKLMPISFFDKALLNGRNLAETIIGHIKEFSSLRLPKHRSVLNAFTHITATIIAY
                     QINPLPPQPIRAFVPN"
BASE COUNT          320 a          466 c          453 g          365 t
ORIGIN      
        1 atggaactga tcgccagccg tcgcctgccg ttggtgcttg gcctgcttgc ctcgtccgcg
       61 ttcaccggac tggcggcgac cgccaccagc gccggtgcag ccgtcacctt catcgcgctg
      121 tcgatgttct gcatgcagtt cggcattacc tgcaaatgga tcctggtgac ggcggtcacg
      181 ccgcaggctt actgcgcctc cgccgccagc aaccagaatt tcgccggatt cctcggcggc
      241 accctttcgc ccatcctgac cggctttatt gtcgatatga cgggctcctt cgtgctggcg
      301 ttagcgatcg gtgcggtgat cacgctgatc ggtgcggcgc tgtttcaatt tatgatgacg
      361 acggtgatcg acgagggcga gttggagaaa tacgcccatg cgccggcctg acatgcaaac
      421 ctgatcctga gcatgatctc gctggacggg aggtctggag cgtgtcgatc ctggctgcga
      481 ccgcatcgtg gcctctctca ggacaagctg ccgctctatc ttaacccgag ctacgggttt
      541 gagttgtgct aaccttgtgg aaactttgtt ggattggtta ggtcggcgac gccaacgcgg
      601 ggaggcaggc atgttcgacc agctcgtcga aaggttctgt gaatttgacg atttttgcca
      661 ggcggttcgt ccgcaatggg aagcgacgtt gctgacagag ggtaaggcgg gacgacgcaa
      721 acccgggccg gaacgaggat tggccgactc tgaagtcatg acgcttttgg tgctctatca
      781 cagctcgcgc ttcaagcata tcaaggcgtt ctacaatggt gttgttctaa caatactgcg
      841 tccttatttt ccggacgctc cttgttatga gcgtttcctg agcatcacaa gttccgtatg
      901 gccgctgttg gtcttctttc tcgtcagccg catgggcgcc aggacgggtt tctattacat
      961 tgacagcacg ccgctggccg tgtgccacaa ccgccggatc agtcgccaca aggttttcgc
     1021 cggattggcg gagcgcggga aaaccagcat cggctggttc ttcggcttca aactgcatct
     1081 ggtgttcaat catcagcgtg aaatcgtcgc actcaagctg accccaggca acgtcagcga
     1141 taccgggccg gtgccggaac tgacgcaagg cctgatcggc aaactcttcg gcgataaggg
     1201 ctatatcggg agagaactgg cgcaggaact gctgcggcgg ggactggctt tgatgacccg
     1261 cgtcagaaag aacatgaagt tgatgccaat atccttcttc gataaagcct tgctcaacgg
     1321 acgcaacctt gccgaaacca tcatcggcca catcaaagaa ttctcatcgc ttagactgcc
     1381 gaagcatcgc tcggtcctta atgctttcac ccacatcacc gccacgatta tcgcctatca
     1441 aatcaacccc ctgccccctc agcctatccg cgcttttgtc ccaaattgaa cccgtagctc
     1501 gggttaacta cgccgaacgc caccgtgccg ggttgagggt tgggaccgcg atcaccgagg
     1561 ggacagccaa tttcatggtg aatcgccgaa tgaataaatc gcag
//
LOCUS       DASWQN010000412         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1094994, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000412 DASWQN010000000
VERSION     DASWQN010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094994"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VGC82_06755"
     CDS             complement(<1..602)
                     /locus_tag="VGC82_06755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423009.1"
                     /translation="MSVTELGLGDPIAGATPASIGPLEQHHLKSSAFRAASDNTRVTA
                     THWHIAAANGLGWGFDGMDGVIFAVVAPFVIKEFAVDLSTYRSGVQIAMLIGIVGLYF
                     WPWLADKFGRRNLLAINIAMFSISMPLVAMSSSFWMFVGMYALVRFALNGEWAIGSML
                     VAETWPARLRGRVISADRSTWGIGAALAGGIAAYIVENFG"
     gene            complement(801..2207)
                     /locus_tag="VGC82_06760"
     CDS             complement(801..2207)
                     /locus_tag="VGC82_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0423010.1"
                     /translation="MPQEALRSILPSAGWSADRAQAVRFTNGTDPILPTPFRVGVAGA
                     ATLAATGLAAADLWQTRTGRQQDIEVDVRQATASLRSGHYLKVGDGDVAAGRNSIMGF
                     YPAKDGHWSYIHANFPNHRAAALKVLGCEENRDSVARAVATWNAADLEEAIIAAKGAG
                     GMVRTRAELASHPQAAAIAALPLMEIVRIGDSPPEPLPEGNRPLSGVRVVDVTRVLAG
                     PTCARTLAEHGADVMKISAAHLPNIGYQEFDTGHGKLSTHLDLREQSGVDTLRDLVRQ
                     ADVFSQGYRPGTLGARGLSPEELAALRPGLVYVSLCAFGHTGPWASRRGFDTVIQSVS
                     GITARQGEVFPGKTPGPQFYPVSAIDYCTGYLMAAGAMVALQRRAEQGGSWLVRISLA
                     QVGKWIVDLGEVPAEALKGVAEEFTPDELKRWSMVSETPSGRLQHLKPVVQLSETPPH
                     WARPSVPLGYHPPVWPAS"
BASE COUNT          450 a          791 c          741 g          375 t
ORIGIN      
        1 catccgaaat tctcgacgat ataagccgcg atccctccgg ccagcgcggc accgatgccc
       61 caagtggagc ggtcggcgct gatgacgcgt ccgcgcaacc gggctggcca ggtttccgcg
      121 accagcatcg agccgatggc ccattccccg ttcagtgcga accggaccaa ggcatacatg
      181 ccgacgaaca tccagaaact ggaggacatc gccaccagcg gcatggagat cgagaacatc
      241 gcgatgttga tggcgagcag gttccttcgc ccgaacttgt ccgccagcca cggccaaaag
      301 tacagtccga cgatgccgat caacatggcg atctgcacgc cggagcgata tgtgctcaga
      361 tcgaccgcga actccttgat cacaaacggc gcgacgacgg cgaagatcac gccgtccatg
      421 ccgtcgaagc cccagcccag gccattcgcc gcagcgatgt gccaatgggt tgcagtgacc
      481 ctggtgttgt ccgacgccgc gcgaaaagcg ctggatttca ggtgatgctg ttccagcggg
      541 ccgatcgacg cgggcgtcgc gccggcgatc ggatcgccca agccaagttc agtcaccgac
      601 atggattgcc tctccccgtc ccgatctccg gcctttggcc gtgagccggt tcatgcgacg
      661 cgccaggagc caaattgtca acgccgcagg tgggagacac cggagtaaga caggccggag
      721 taagacaggc caaagtgagt cagaccaatg tgagacagac caaagtaaga caggccgggg
      781 tcgggtcacc aggcagtgga tcacgacgcc ggccagaccg gcgggtgata acccagcggg
      841 acggatggcc gagcccaatg aggcggtgtc tcggacaact gaaccacggg ttttagatgt
      901 tgcagccggc ccgacggcgt ctcgctgacc atcgaccagc gcttcagttc atcgggcgtg
      961 aactcctctg ccacgccctt cagggcctcc gccggaacct caccgagatc gacgatccac
     1021 ttgccgacct gagccagcga aatccgcacc agccaactgc ccccctgttc ggcgcgccgc
     1081 tgcaacgcga ccatcgcccc agcggccatc aggtagccgg tgcagtagtc gattgccgac
     1141 accgggtaga actgcggtcc gggggtcttg cccggaaaga cctcgccctg ccgcgccgtg
     1201 atgccgctga cggattgtat gaccgtgtcg aacccgcgcc gtgaggccca agggccggtg
     1261 tgcccgaacg cgcacagcga gacatagacc agccccgggc gcaacgccgc cagttcctcg
     1321 ggcgacaagc cacgcgcgcc cagcgtcccc ggacgatacc cttgcgaaaa aacatccgcc
     1381 tgccggacaa ggtcgcgcag cgtatcgacc ccgctttgct cgcgcagatc gaggtgggtg
     1441 gacagcttgc cgtgaccggt atcgaactcc tggtagccga tgttgggcag atgcgcggcg
     1501 gaaatcttca tcacgtcggc accatgttcc gcgagcgtgc gtgcgcaggt cgggccggcc
     1561 agcacgcggg tcacatcgac gacgcgcacc cccgacagcg gccgattgcc ttcaggcagt
     1621 ggttccggcg ggctgtcgcc gatccgcacg atttccatca acggcaacgc ggcgatggcc
     1681 gcggcttgcg gatggctcgc caattccgca cgggttcgca ccatgccacc cgcccctttc
     1741 gcggcgatga tcgcttcctc cagatcggca gcgttccacg tcgccacggc gcgggccacg
     1801 gagtcgcggt tctcctcgca gcccagaacc ttcaacgcgg ccgcccgatg gttggggaaa
     1861 ttggcatgaa tgtagctcca atggccgtcc ttggcggggt agaaccccat gatcgagttg
     1921 cgccccgcgg cgacatcgcc atccccaacc ttcaggtaat ggccgctgcg gagcgatgcc
     1981 gtcgcctggc gcacgtccac ttcgatatcc tgctggcgac cggtccgggt ctgccacagg
     2041 tcggccgcgg cgaggccggt ggcggccaag gtcgctgccc cggccacgcc gacacgaaac
     2101 ggcgttggca ggatgggatc ggtgccgttg gtgaaccgga cggcttgcgc gcggtcggcg
     2161 gaccaaccgg cactcggcag aatgctgcgt aaggcctctt ggggcatcga tatcatctcc
     2221 cgatcgtctt cttcggttgg atcatgctcc gtttcatcag gaccgcaaat tgggaggccc
     2281 acgaatcggg aggactgcaa atcgggagga ccccaaatcg gggccgcgcg gtcctgtgac
     2341 caaccgactg atagttc
//
LOCUS       DASWQN010000413         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1359678, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000413 DASWQN010000000
VERSION     DASWQN010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359678"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VGC82_06765"
     CDS             complement(<1..395)
                     /locus_tag="VGC82_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0423011.1"
                     /translation="MGLPRADAETKAWFMAWLEGFAEHVRAVDYGPARKLFHQDILAF
                     GTHQDVLPSLQAWITTQWDNVWPRTDDFRFTIAEAHVLVSDDNTMAVVVAPWTSTGFD
                     VHGTPFDRPGRATIVFHRSDNLWLGVHSHL"
     gene            complement(407..1603)
                     /locus_tag="VGC82_06770"
     CDS             complement(407..1603)
                     /locus_tag="VGC82_06770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423012.1"
                     /translation="MQRTEVRSLALICSAHLISHFYYLVLIPLFPMLKARLGLGFVEL
                     GFAITLSNIIGGIVQTPMGYAVDRFGARRVLIAGLILGGAAYISIGLFPVYSWILVAM
                     VLVGVANAVYHPADYAILGAVIEPSRLGKAFSIHTFAGFLGSAIAPILMLLGAQAFGF
                     QAAVIAAGVVGIAVAVPLGMAGWLDRHAASRSGAEAAQHIPMRKLLTPSVLGLVGFFA
                     LLSLSSGALTNYSAVALVTVYGISLSSANLALSAFLFASAVGVLAGGVVADRTRRHGD
                     VAAFGYGGAAVLILLVGTFDLGSILLVAAMGGAGFLSGMISPSRDMLVRAASPPGAFG
                     RVFGIVTTGFNLGGTVGPLIGGWIMDHNLPRWVFFSSVLFMALTSVMALASDWRLRRR
                     SSLALS"
     gene            complement(1937..>2144)
                     /locus_tag="VGC82_06775"
     CDS             complement(1937..>2144)
                     /locus_tag="VGC82_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY0423013.1"
                     /translation="TWRSGGFAPIREQWLKRSYPIGAAIRATAGGEPVAGDFAGLDLD
                     GALLLDTPQGRRRFVAGDITVAGG"
BASE COUNT          450 a          706 c          670 g          318 t
ORIGIN      
        1 agatgggaat gaacgcccag ccatagattg tccgacctat ggaacacgat cgtggcgcgg
       61 cctggacgat cgaacggcgt gccgtgaaca tcaaaccccg tacttgtcca cggggcgacg
      121 acgacagcca tggtattgtc gtccgacacc agaacatgcg cttcggcgat cgtgaagcga
      181 aagtcgtcgg tcctcggcca cacgttgtcc cactgcgtcg taatccaggc ctgcaggctc
      241 ggcagcacat cctgatgggt gccaaacgcc aggatgtcct ggtggaacag cttccgcgcc
      301 ggaccgtaat cgaccgcgcg aacatgttcg gcgaacccct ccagccacgc catgaaccat
      361 gctttggttt cggcgtcggc gcgtggcaaa cccatggcgc ttactcctac gaaagtgcca
      421 gcgacgaccg ccgccgcagc cgccagtcgc tggccagcgc catgaccgac gtcagcgcca
      481 tgaacaggac cgacgagaaa aacacccagc gcggcaagtt gtggtccatg atccagccgc
      541 cgatcagcgg accgaccgtg ccgccaaggt tgaagccggt cgtgacgatg ccgaacaccc
      601 gcccgaacgc gcccggcggc gatgccgcgc gaaccagcat atcgcgcgac ggcgatatca
      661 tcccggacag gaaccccgcc ccgcccatgg ctgcgacaag caggatggaa ccaaggtcga
      721 aggttccgac caacaggatc aacacggccg cgccgccgta gccgaaggcg gcgacatcgc
      781 cgtgacggcg cgtccggtcg gcaacgacgc cgccggccag gacaccgacc gccgaggcga
      841 acaggaacgc cgacagcgca aggttcgccg aggacagcga gatcccatag acggtgacca
      901 gggcgaccgc cgagtagttc gtcaacgcgc cgctgctgag gctgagcagc gcgaaaaagc
      961 cgacaagccc aaggaccgaa ggcgtcagca atttccgcat tgggatatgt tgtgccgcct
     1021 ccgcccccga gcgggaggcg gcgtgacgat ccagccagcc agccatgcca agcgggacag
     1081 cgaccgcgat acccaccacg ccggcggcga tgacggcagc ctggaaacca aaggcctggg
     1141 cgcccagcag catcaggatc ggggcgatgg cgctgcccag gaatcccgcg aacgtatgga
     1201 ttgaaaacgc cttgcccagc cggctcggct cgatcaccgc accgaggatg gcgtaatcgg
     1261 ctgggtgata gaccgcgttg gcaacaccga ccagcaccat ggccaccagg atccatgaat
     1321 agaccggaaa tagcccgatc gagatatagg ccgcgccgcc caggatcagg ccggcgatca
     1381 gcacccgcct cgcgccgaag cgatcgaccg cgtaacccat cggcgtctgg acaatcccgc
     1441 cgatgatgtt ggacagcgtg atcgcgaagc caagctcgac gaagcccagg ccgaggcggg
     1501 ctttcagcat cgggaaaagc ggtatcagca cgaggtagta gaaatggctg atcagatgcg
     1561 ccgaacaaat gagggcgagg gatctgactt ctgttcgttg caaacgcgtg ttcattcgtc
     1621 actcttgcgc atcatggctt cggtgcgctg cctatcacat tggtggccct ggggccgttg
     1681 atttctgcct ggctcgggcc tcggccgcgt tggatcgctt gttgcagccg cgaacctgga
     1741 tcgcagggca cttttatcca atcccgcctg cccatggaca gacggcgttg gggcgatgtg
     1801 atccattcgg tctgtgctct tgtgccggac gttccggcga acgttcccat cgatggggct
     1861 cacggtcaaa tagaacacgg ggtcgaacgt ctatctcgac aacacccgat gccgccgaag
     1921 ggtcaatcgg ctgactttat ccgccggcca ccgtaatatc gccggcgacg aagcgtcgcc
     1981 gcccctgcgg cgtgtcgagc agcaaggcgc cgtccagatc cagcccggca aaatcgccgg
     2041 ccaccggttc accgccggcg gttgcccgga tcgcggcacc gatcgggtag gaccgtttga
     2101 gccactgctc gcggatcggc gcgaaaccgc cggagcgcca tgtg
//
LOCUS       DASWQN010000414         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1755614, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000414 DASWQN010000000
VERSION     DASWQN010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755614"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1841)
                     /locus_tag="VGC82_06780"
     CDS             complement(108..1841)
                     /locus_tag="VGC82_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423014.1"
                     /translation="MSVSAAQAAGVSSATRAMTPDEKKIILASSLGTVFEWYDFYLYG
                     SLAVIIGAKFFGQFDETTRNIFALLAFAAGFLVRPFGALVFGRLGDLVGRKYTFLITI
                     LIMGASTFLVGILPTSDSIGITAAIVLISLRILQGLAIGGEYGGAATYVAEHAPSGRR
                     GFYTSWIQTTATLGLFMSLLVILAVQAAMSADDFKAYGWRIPFLVSVLLLAVSVWIRL
                     QLSESPAFLKMKADGTHSKAPLSEAFGQWANAKWAILALFGLVAGQAVVWYCGQFYAL
                     FFLTGVLKVDGFTGNLLVAWALVVGALGFILFGWLSDKVGRKPIILGGCLLAVLTYFP
                     VFKFMAETANPALSSAHETVHVVVVADPADCSFQFNPTGLSKFTNSCDQAKGALSRSS
                     VTYSQDDAPPGTKASVKIGDKTLDPTSKNFAADLSSTLTSVGYPAASNPSVVKISNPF
                     DIFRPQPFKIVLCLCFLMVLVTMVYGPMAAALVELFPTRIRYTSMSLPYHIGNGWFGG
                     LLPASMFAMNAQNGSIYFGLWYPIVIAGATVVIGLLFIPETKNRNIFAGDSSTHPGDT
                     RIDFDPNAADY"
BASE COUNT          538 a          722 c          746 g          426 t
ORIGIN      
        1 gggttcggcg ggaagcgcga ttccgcagag ggaatttgaa agagggaatt tgaaccgtgt
       61 ccaactcgcg tgggtggctc aacgcgggct ggacacggtt catgtcatca gtaatcggcc
      121 gcgttcgggt cgaaatcaat cctcgtatcg cctggatggg tcgatgaatc gccggcgaag
      181 atgttgcgat ttttggtttc gggaatgaac aacagaccga tgaccacggt cgccccggcg
      241 atcacgatgg gataccacaa tccgaagtag atgctgccgt tctgggcgtt catcgcgaac
      301 atcgaagccg gaagcaggcc accgaaccag ccgttgccga tgtggtaggg cagcgacatc
      361 gaggtatagc ggatccgcgt tggaaacagt tcgaccaggg cagcggccat cggaccatag
      421 accatggtga caagcaccat caggaagcac aggcaaagca cgatcttgaa cggctgcggt
      481 cggaagatgt cgaacggatt ggagatcttg acgaccgatg ggttcgacgc cgccgggtag
      541 cccaccgacg taagcgtgga agaaaggtcg gcggcgaagt tttttgaggt cggatccagc
      601 gttttgtcac cgatcttcac gctcgccttc gttccgggcg gtgcatcgtc ctgggagtac
      661 gtcaccgaag acctggacaa tgcgcccttc gcctggtcgc agctattggt gaacttggat
      721 aggcccgtcg ggttaaactg gaacgaacaa tccgccgggt cggctacgac cacgacatgc
      781 accgtctcgt gcgcgctgga cagcgccgga ttggcagtct cggccatgaa tttgaagacc
      841 gggaaatagg tcagcaccgc gagcaggcag ccgcccagga tgatcggctt gcggccgacc
      901 ttatcggaca gccagccgaa caggatgaac cccagcgcgc ccaccaccag cgcccatgcc
      961 accagcaaat tgccggtgaa accgtccacc ttcaacacgc cggtcaggaa gaacagggca
     1021 tagaactgcc cacaatacca gacgacggcc tggccggcga cgaggccgaa cagagccagg
     1081 atcgcccatt tggcgttggc ccactggccg aacgcctcgc tcagcggtgc cttggaatgg
     1141 gtcccgtcgg ccttcatctt caggaacgcg gggctctcgc tgagctgcag gcgaatccag
     1201 accgaaaccg ccagcagcag caccgaaacc aggaacggaa tgcgccagcc ataggctttg
     1261 aaatcatccg cgctcatggc tgcctggaca gccaggatca ccagcaacga catgaacagg
     1321 ccaagggtgg cggtggtctg aatccagctt gtgtagaagc cgcgccggcc ggacggggcg
     1381 tgttcggcca cataggtcgc tgcgccgccg tattcgccgc cgatcgccag cccttgcagg
     1441 atacgcaagc tgatcagcac gatcgccgcg gtgatgccga tgctatcgga cgtgggcagg
     1501 ataccgacga ggaaagttga cgcgcccatg atcaggatcg ttatcaggaa agtgtatttg
     1561 cgtcccacga ggtcgcccag ccgcccaaac acaagcgcgc cgaacgggcg caccagaaac
     1621 ccggcggcaa acgccagcag agcgaagata tttcgcgtcg tctcatcgaa ctggccaaag
     1681 aatttcgcgc cgattatgac tgcgagtgag ccatacaggt aaaaatcata ccattcgaag
     1741 acagtgccaa gtgatgatgc aaggatgatt ttcttctcat ccggcgtcat cgcacgagtg
     1801 gccgaggaaa ccccggcagc ttgggccgcg cttacgctca tgattctttt tgcctctccc
     1861 aaaaaccttg tcgatcggcc tcgttcgccg ttttggcggc caatccagcc ggaaaccgcc
     1921 actggacgga atggcgggag gccggtcctt agctgaaatc ggggatgcct gacagaggaa
     1981 aattacgtcg gcattaccac cttggccatg aaaccagggt cgcccgcgat cgcttgagag
     2041 gttggcgaca tcttcaatcc aggtcgtttc attgcaagac gatccacctg tggtgccgcg
     2101 gtcgcgggat agccgtggac gctgaagcgg aagaacgtct cgagcggagg aagtgcgctg
     2161 cgccggcagc gatcatgcaa ctgtcgatcc gccatccgtc gtgcttcacg ctatgcgaca
     2221 acctcctccc ggtcggcgct gtcccggcca aagcgcccgt ccgaggagtg tttatgcccc
     2281 cggccgtgaa aatctggata gcgtcaccca cactccgccc gctgaccgga gcaggccgac
     2341 cgggatatga atgggatgat cgcggcgccc gcctgtggtg atttcagcag ccgtacgctc
     2401 agcggcggtg cgacggtccc gaagcaatca cc
//
LOCUS       DASWQN010000415         6622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2283338, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000415 DASWQN010000000
VERSION     DASWQN010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2283338"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1973)
                     /locus_tag="VGC82_06785"
     CDS             complement(180..1973)
                     /locus_tag="VGC82_06785"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0423015.1"
                     /translation="MAARAIAIRPSSPQSLPQKEDQSMADIVIRNGTVIDGSGGAPFE
                     ADIAIENGRIAAIGRIAARGTEEIDARGQIVTPGFIDPHSHYDAQATWSSRITPSSWH
                     GVTTTLIGNCGVGFAPCRPDQRQMLVRLMEGVEDIPEPVLTEGLKWNWQSFPDYLDAL
                     DARPYDLDIATQVPHAAVRVFAMGQRGLDREPATDADSAAMARIAAEGIRAGALGFST
                     SRTINHKTKAGEHIPTLDAEEAELTAIARALRGIDAGWLQVISDFDDAEPEFAMLRRL
                     TEAAGRPMAITILQRDSKPEEWRRITGWIAEANAAGVKMLGQVLTRATGILLGLEISQ
                     NPFLGRPSFAAVADLPFERKIAALRDPSLRARLLAEEGPDPVLERRVSRWDRIFPLTD
                     SPDYEPAPENSIAARAVREDRDPADLAYDLLLEDNGRALFYRPLSNYSYGNLDTVHDM
                     LAHDATIVGLGDGGAHVGILCDSSAISTMLTHWTRDRSRGAKFGLPWAVKRITRDSAQ
                     AIGLLDRGLLAAGYKADLNVIDYDRLRLRKPEVVYDLPSGGRRLIQRADGYTATIVAG
                     QVTYRDGTPTDALPGRLLRGAQSAPAAMAAE"
     gene            complement(1970..3781)
                     /locus_tag="VGC82_06790"
     CDS             complement(1970..3781)
                     /locus_tag="VGC82_06790"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEY0423016.1"
                     /translation="MITGIEIKSRSPLVGGAAFGGVGPYERIEGVASGELDPAHPGNQ
                     AIALLDQAPVNRAGQVEYRSDFVLLRPADAGRGNGRLLYEVNNRGRILLFANLCAGAA
                     GNQPETAEDLGNALPLRLGFSVLWTGWDPGAPKATGLSLDVAAIEGMTQNIREEFISG
                     TRLGAHQAFKLAYEAVGPAAVTVRRTQTAARVPVASEMVDNRTVRPVAAVELGSIYEV
                     RYQATKPRVLGIGFAATRDVVSYLRRDGAELLGRPVEHTLAFGISQAGRYLRDHIARG
                     FNADEDGRKVFDGVFTHVAGIGRVFHNTPFGQPFRTRTWHEDHDFPEIEFPHSSASMD
                     DPVTGRSGALMRGDATDPLLIETNTSTEYWQKGASLLHTDPLGTQDIALPPNVRAYLL
                     PGTQHGGKAGMPRDNGPCVNPRNWHDPTPAIRALLVALDEWVVSGRSPPDSLVPRIDD
                     GTLVSADKVAFPAIPGLVRPLAANNAPPMGDWTDPSPPAAAYATLVPQVDADGNEIAG
                     IRLPDIAVPKGTFTGWNLYKAPYPDGELADRDGTFLAFAATEAERGDDPRPSLAARYN
                     AATYAEAVRAVVQDLMRERFLLPEDAERYVAQAGYKL"
     gene            complement(3778..4425)
                     /locus_tag="VGC82_06795"
     CDS             complement(3778..4425)
                     /locus_tag="VGC82_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEY0423017.1"
                     /translation="MAFKPLDRSGIAARIAQDIPEGWFVNLGIGIPTAVSDHVPLERE
                     VIFHSENGVIGAGPAPDPDKVNPYLVNAGTQHITLRTGGSFVHHADSFAIARGGHLDL
                     CVLGAFEVAQNGDIANWARSPDEPVKQVGGAMDLAIGAKCLWVAMEHNTKGNGESRIR
                     RACSYPLTAKGVVRRVYTDIAVLDVTPKGFVVRDMIPGMTRQELQERSEAELHWT"
     gene            complement(4437..5093)
                     /locus_tag="VGC82_06800"
     CDS             complement(4437..5093)
                     /locus_tag="VGC82_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEY0423018.1"
                     /translation="MIDKFVPDVAAALAGIEDGATILLAGFANGMPETLMEGLIKQGA
                     RNLTLVVNAGGRDTGPIAELLGTGRVNKLICSFVRADSVAGRRFAAGTLDLEIVPQGT
                     LAERIRAAGAGVQAFYTPTGADTLLADGRETRVINGRNCLLEYPLYGDVALIDAWEAD
                     RWGNLTHRESARNYNPVMAMAAKLTVAQTRHVVPLGDIPPENVHTPGVFVHRVVHIPA
                     "
     gene            5252..5668
                     /locus_tag="VGC82_06805"
     CDS             5252..5668
                     /locus_tag="VGC82_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY0423019.1"
                     /translation="MGRIQELRQKLPDSEKITINLGFVDLGQIDLMVRDGFYANRTDF
                     IRTSIRHQLDRHADAVRQSIARKTLSLGLCNYTRSDLEAARQRGEMLHINVLGLATIA
                     SDVTAELARAAIGSLTVLGALHASAEVRAALADRMH"
     gene            5808..>6622
                     /locus_tag="VGC82_06810"
     CDS             5808..>6622
                     /locus_tag="VGC82_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY0423020.1"
                     /translation="MKFSLPNLMQGATRLTQSGRLTEATAALQRLLEASTPSTGQQGP
                     LRSFRQDASEYANAGPNAAQFLAKTFSNQAGSRPYKLYIPNGYRGQPVPLIVMLHGCT
                     QSPDDFAAGTQMNIAAEAKTCLVAYPGQTSAANMQKCWNWFNQVEQQRAGQEPSLIAG
                     IAQEVMRDYAVDPARVYVAGLSAGGAAAAIMGNAYPDIFAAIGVHSGLACGAARDMPS
                     AFAAMKGGADGSVRLDRRRMVPAIVFHGERDTTVNPRNSDAVIRQLSKDVPLRT"
BASE COUNT         1189 a         2232 c         2020 g         1181 t
ORIGIN      
        1 gatcttcagg aagtcgtcgg agacgccgaa ggcgtgctgc accgcctcat ccagctcatc
       61 cacctcggcc agcgataaca gttcggtcca ggtcgaagga tcggcgacat ccgccgcggt
      121 ccattcgcag gcggtctcaa ggacctgtgg ccggaagggc gtgacgtcgt tcatcgccgc
      181 tactccgccg ccatcgccgc gggcgcgctc tgcgccccgc gcaacaggcg gcccggcagg
      241 gcgtcggtgg gcgtcccgtc gcgataggtc acctggcccg cgacgatggt cgcggtgtag
      301 ccgtccgccc gctggatcag ccgccggcca ccgctgggca ggtcgtaaac cacttccggt
      361 ttgcgcaggc gcaggcggtc gtagtcgatc acgttcaaat ccgccttgta gccggcggcc
      421 aacaaacccc gatccagcaa cccgatcgcc tgcgccgagt cgcgggtaat ccgcttcacc
      481 gcccaaggca gcccgaactt cgcgccgcgg ctgcgatccc gggtccaatg cgtcagcatc
      541 gtgctgatcg cactggagtc gcaaagaatc ccgacatgcg cgcctccgtc gcccagcccg
      601 acgatcgtgg cgtcatgggc cagcatgtca tgcaccgtat cgaggttgcc gtagctgtaa
      661 ttcgacagtg gccgatagaa cagcgccctc ccattgtctt caagcaacaa atcgtaagcc
      721 aggtccgccg gatcgcggtc ctcccgcaca gcccgcgccg cgatgctgtt ctccggtgcc
      781 ggttcgtaat ccggagagtc ggtcagcgga aaaatccggt cccagcgcga cacccgccgt
      841 tccagcaccg gatcgggacc ctcctccgcc agcaagcgag ctcgcaagga cgggtcgcgc
      901 agtgccgcga tctttcgctc gaatggtaga tccgccaccg ccgcgaaact ggggcggcca
      961 aggaatgggt tctgcgagat ctcgagcccc agcaaaatac cggtggcacg cgtcagcacc
     1021 tggcccagca tttttacgcc agcggcgttg gcctcggcga tccagccggt gatgcggcgc
     1081 cattcctccg gcttgctgtc gcgctgcagg atggtgatcg ccatcggccg ccccgccgct
     1141 tccgtcaatc gccgtagcat ggcgaactcc ggttcggcat catcgaaatc gctgatcacc
     1201 tgcagccacc ccgcgtcgat cccccgcaag gcgcgagcga ttgcggtcag ttcggcttcc
     1261 tcggcatcca acgtcggaat gtgttcgccg gccttggtct tatggttgat tgtccgagac
     1321 gtggaaaatc ccaacgcgcc ggctcggatt ccctccgccg cgatacgcgc catggcggca
     1381 ctgtcggcat cggtggccgg ttcccggtcc aatccgcgct gccccattgc gaagacccgg
     1441 acggcggcat gcggcacctg cgtggcgatg tcgaggtcgt acggccgagc gtcgagtgca
     1501 tcgagataat cggggaagga ctgccagttc cacttcaggc cttcggtcag gaccggttcg
     1561 gggatatcct ccacgccctc catcaggcgc accagcatct gccgctggtc tggccggcag
     1621 ggggcgaaac cgacgccgca attgccgatc agcgtcgtgg tgacgccatg ccatgacgat
     1681 ggggtgatac ggctgctcca ggtcgcctgg gcgtcgtagt gggagtgcgg atcgataaag
     1741 ccgggggtga cgatctggcc gcgcgcgtcg atctcttcgg taccgcgagc ggcgatcctg
     1801 ccgatggcgg cgatgcggcc attttcaatg gcaatgtcgg cttcgaatgg ggcgcctccg
     1861 gaaccgtcga tgacggtgcc attgcgaatg acgatgtcgg ccatggattg gtcctccttt
     1921 tgcgggagag actgcggcga actggggcgg atcgcaatcg cccgcgccgc tatagcttat
     1981 agccggcttg cgcgacgtac cgctccgcat cctccggcaa caaaaagcgc tcccgcatca
     2041 ggtcctgaac gaccgcccgc actgcctccg catatgtcgc cgcgttgtac cgggccgcca
     2101 gcgaaggtct cggatcatcg cctcgttccg cctcggtggc cgcgaacgcc agaaacgtgc
     2161 cgtcccgatc cgccagttca ccgtcaggat agggcgcctt atacaaattc caccccgtaa
     2221 acgttccttt cggaaccgcg atgtccggca gcctgatgcc tgcgatctcg ttgccgtccg
     2281 cgtcaacctg aggcaccagc gtcgcatagg ccgcggccgg cggcgatgga tcggtccagt
     2341 cgcccatagg tggtgcgtta ttcgccgcaa gcggccgcac cagcccggga attgccggaa
     2401 aagccacctt gtccgctgaa accagcgttc catcgtcgat ccggggcacc aggctgtccg
     2461 gcggcgaccg ccccgaaacc acccactcat ccaaagccac cagcagcgcc cggatcgcgg
     2521 gcgtcggatc gtgccagttc cggggattga cgcacggccc attgtcccgc ggcatccccg
     2581 ccttgccgcc atgctgggtg cctggcagca ggtaggcgcg cacgttcggc gggagtgcga
     2641 tgtcctgcgt ccccaacgga tcggtgtgca gcagcgacgc ccccttctgc cagtactccg
     2701 tcgaggtgtt cgtctcgatc agcagcgggt ccgtcgcatc gcctcgcatc agcgcgccgc
     2761 tccgcccggt aaccggatcg tccatcgagg ccgacgaatg cgggaactct atttctggaa
     2821 agtcatggtc ctcgtgccag gtgcgtgtgc gaaatggctg tccgaacggc gtgttgtgga
     2881 acacccgccc gatcccggcg acgtgggtga acacgccgtc gaacaccttc cgcccgtcct
     2941 cgtcggcatt gaagccccgg gcgatatgat cccgcaggta acggcccgcc tgcgaaatgc
     3001 cgaaggccag cgtatgctcg acaggccgtc ccagcagctc cgcgccatcg cgccgcaggt
     3061 agctgacgac atcccttgtc gcggcgaacc cgatgcccag aacccgcggc ttcgtcgcct
     3121 gatagcgcac ttcatagatc gacccgagct cgaccgcggc caccggtcgc acggtccggt
     3181 tatcgaccat ttcggacgcc accggcacac gcgcggccgt ctgcgttcgc cgcaccgtta
     3241 cggccgccgg tccaacagcc tcgtaggcca gtttgaacgc ctgatgcgca cccagccggg
     3301 tgccggagat gaattcctcg cggatgtttt gggtcatgcc ttcgatcgcc gccacatcca
     3361 gcgacaatcc tgtcgccttc ggtgcgcccg gatcccatcc tgtccacaat acggaaaatc
     3421 ccagacgcag cggcaatgcg ttgcccaaat cctcggcggt ttccggctgg ttgccggccg
     3481 cacccgcgca cagattggcg aacagcagaa tccgcccgcg attgttcacc tcatacaaca
     3541 gccgcccgtt gcccctgccc gcatccgccg gccgaagcag cacgaaatcc gagcgatact
     3601 cgacctggcc ggctcggttg accggcgcct gatccagcag cgcaatcgcc tggttcccgg
     3661 gatgggccgg gtcaagctcg cccgacgcga cgccttcaat ccgctcatac ggaccgacgc
     3721 cgccgaacgc cgccccgccc accaaaggcg agcggctttt gatttcgatt ccggtgatca
     3781 tgtccagtgc aactccgctt cgctgcgttc ctgcaattcc tgacgagtca tgccggggat
     3841 catgtcccgc accacaaagc ccttcggcgt cacgtccaga accgcgatgt ccgtgtagac
     3901 acgccggact acacctttcg ccgtcaacgg ataggaacac gcacgccgga tgcgcgattc
     3961 gccgttgccc ttggtgttgt gctccatcgc aacccacagg cacttcgcac caatcgccag
     4021 atccatcgcc ccgccaacct gcttcaccgg ctcatcgggc gagcgtgccc aattggcgat
     4081 atcgccgttc tgcgcgacct cgaacgcgcc cagcacgcat agatcgagat gcccaccgcg
     4141 agcgatggcg aagctatcgg catgatgcac gaacgagcca ccggtgcgca gcgtgatgtg
     4201 ctgcgttccg gcgttgacga ggtaagggtt gaccttatcg ggatcaggcg ctgggccggc
     4261 gccgatcacg ccgttctcgg agtgaaagat cacctcccgt tccagcggca cgtggtcgga
     4321 cacggcggtg ggaataccga tgccgaggtt gacgaaccaa ccttcgggaa tgtcctgcgc
     4381 gatgcgggca gcgatgccgc ttcgatcgag tggtttgaat gccatggcta cctcgttcag
     4441 gccggaatat gcacgacgcg gtggacgaac acgccgggcg tgtggacatt ctccggcggt
     4501 atgtcaccca gcggaacgac atgccgggtt tgcgcgacgg tcagcttcgc cgccatcgcc
     4561 atgaccgggt tgtagttccg ggcactctcc cggtgggtca gattgcccca gcgatcggct
     4621 tcccacgcat cgatcagcgc gacatcgcca tacagcggat attccaacag gcaattgcgc
     4681 ccgttgatca cgcgggtttc acggccgtcc gcaagcaacg tgtccgctcc ggtcggcgta
     4741 tagaacgcct gcacgccggc acccgccgca cgaatgcgtt cggccagcgt gccctgcggc
     4801 acgatctcca ggtccagcgt accagccgcg aagcggcgcc ccgctacgct gtcggcgcga
     4861 acgaaactgc agatcagttt gtttacccgg ccggtcccca acaattcggc tatcggcccg
     4921 gtatcgcggc caccggcatt caccaccagg gtcagattcc gagccccctg cttgatgagg
     4981 ccttccatca gggtttccgg catcccgttg gcaaagcctg ccaacaggat cgtggccccg
     5041 tcctcgatgc cggccagcgc ggccgccaca tcgggcacga acttatcgat cattcgaagg
     5101 tactttcttg aattccagga cgccgcttcg cttgtatagg agcgcggaac gacggcgctg
     5161 gcaacggaac cgcccccgga gccggcggca actgcttgta aatcggaagc tcagtgttat
     5221 atccatctac atggatggta aggagatggt ggtgggcagg attcaggagt tgcggcaaaa
     5281 gctgccggac agcgagaaaa tcaccatcaa cctcggcttt gtggatcttg gacagatcga
     5341 cctgatggta cgggacgggt tttacgccaa ccggacggac ttcatccgca catcgattcg
     5401 gcatcaactc gaccgccacg ccgacgccgt ccgccagtcg atcgcgcgga agacccttag
     5461 ccttgggcta tgcaactaca cccgtagtga cctcgaggcc gctcggcagc gcggcgaaat
     5521 gcttcacatc aatgtgcttg ggctggcgac gatcgcctcc gatgtaacgg cggaactggc
     5581 ccgagccgcg atcggttcgc taacggtgct aggcgcgttg catgcgagcg ccgaggtccg
     5641 ggctgcgctg gcggatcgta tgcattgacc accgcgatcg accagcaaac ccattcatct
     5701 ggagcgtgag cccacgtttc cgaacataat tttggccagc ttaactttgg ccagcttaac
     5761 tttggccagc ctaactttgg gccagcctaa ctttggaaag tacaagcatg aaattttcct
     5821 tgccgaacct catgcaaggg gccacgcgcc tgactcagtc cggccgcctg acagaggcaa
     5881 ccgcggcact gcaacgcttg cttgaagctt cgacgccctc gacaggtcag cagggcccgt
     5941 tgcggtcgtt ccgtcaggac gcctccgaat atgccaatgc cgggccgaac gcagcgcaat
     6001 tcctggcgaa gaccttcagc aatcaggcgg gaagccgccc ttacaaattg tatataccca
     6061 atggctaccg agggcagccg gttccgctga tcgtcatgct gcacggatgc acccagtcgc
     6121 cggacgattt cgccgcggga acgcagatga acatcgcggc cgaggcgaag acgtgcctgg
     6181 ttgcctatcc gggccagacc agcgccgcga acatgcagaa gtgctggaat tggttcaacc
     6241 aggtggagca gcagcgtgcc ggtcaggaac cgtcgctgat cgccgggatc gcgcaggaag
     6301 tcatgcgcga ttacgcggtc gatcctgcgc gggtttatgt ggcgggcttg tcggcggggg
     6361 gtgctgccgc ggccatcatg ggtaacgcct atcccgatat tttcgcggcg atcggagttc
     6421 actccggtct ggcttgtggc gcggcacggg acatgccgtc cgcattcgcc gcgatgaagg
     6481 gcggagcaga cggttccgtc cggctggatc ggcgacgcat ggtgccggcg atcgttttcc
     6541 acggcgagcg cgacacgaca gtaaacccgc gtaacagcga cgcggtcatc aggcagttgt
     6601 ccaaggacgt gccgctacgg ac
//
LOCUS       DASWQN010000416         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1623646, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000416 DASWQN010000000
VERSION     DASWQN010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623646"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..205)
                     /locus_tag="VGC82_06815"
     tRNA            complement(132..205)
                     /locus_tag="VGC82_06815"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(171..173),aa:Cys,seq:gca)
     gene            complement(332..1189)
                     /locus_tag="VGC82_06820"
     CDS             complement(332..1189)
                     /locus_tag="VGC82_06820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY0423021.1"
                     /translation="MLKFGVSMFFTDYSMAPGELGQALEQRGFESVWAPEHSHIPLSR
                     KSPFPTGGDLPKKYYDTMDPFVTLTAAAAATKTLMVGTGVCLVAQRDPIQTAKLVASI
                     DQVSGGRFLFGIGNSWNQDELEDHGIKFADRHKAARERVEAMKEIWTKSKAEYHGEFV
                     NFDPMMAWPKPVQKPHPPVIVGGAFPYSARRAIRYGNGWIPQAARGGYKEIAELIPEF
                     RKMATEAGRNPDEIAITVWFPRRELDLMKRYADLGVERVVFTIESEKADKALAAVDEI
                     AEFMRKVNA"
     gene            1336..>1758
                     /locus_tag="VGC82_06825"
     CDS             1336..>1758
                     /locus_tag="VGC82_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0423022.1"
                     /translation="MSEPARDPASPSAPEKSTAAKSTPVKITDVAAAAGVAPMTVSRV
                     INTPDRVSPETTARVKEAIDRLGYVPNLIAGGLSSRKSRMVAAIVPTIAHPMFAGLVQ
                     AFSASMRHSGYQVMLSISGYGETDDEALFRALLGRRPDA"
BASE COUNT          318 a          585 c          522 g          333 t
ORIGIN      
        1 ctcgatcagc gagtcgcaaa tggccgcgtg gctgcgcgca gccatacagc gtaaacagta
       61 gacgatcgcc ggtaacgtaa ccgggttgta cgccgcagcc gcgccttcag gccgctctgg
      121 gccaactggc ttggaggtcc gtcccggagt cgaaccgggc tccacagatt tgcagtctgg
      181 ccgatcaccg ctctcgcaac ggaccttcgg acgcaggata tacggtctgc aacgccgcgc
      241 ggcaagccca ctggacaacc ccaagcaacg cccgagcgat acccatccgc gcatctgacc
      301 cggcggatct gcttaagacc gcgcaaaagc gttacgcgtt caccttgcgc atgaattcgg
      361 cgatctcatc gaccgccgcc agggctttgt ccgctttctc tgactcgatg gtgaacacca
      421 cccgttccac gccaaggtcg gcatagcgct tcatcaggtc cagttcgcgc cgaggaaacc
      481 acaccgtgat ggcgatctca tccgggttgc ggccagcttc ggtggccatc ttgcggaact
      541 ccgggatcag ctcggcgatt tccttatacc caccgcgcgc tgcctgagga atccagccat
      601 tgccatagcg gatggctcgt cgcgcggaat acgggaatgc gccgccgacg atcaccgggg
      661 gatgcggctt ttgcactggc ttcggccacg ccatcattgg gtcgaagttg acgaactcgc
      721 catgatactc ggccttcgac ttggtccaga tctccttcat cgcctcgacc cgttcccgag
      781 cggccttgtg ccggtcggcg aacttgatgc cgtggtcctc cagctcgtcc tggttccaac
      841 tgttgccgat gccaaacagg aaacgcccgc cgctgacctg atcgatcgag gcgaccagtt
      901 tcgccgtctg gatcggatcg cgttgcgcca ccaggcaaac gccggtgccg accatcagcg
      961 tctttgtcgc cgccgccgcg gcggtcagcg tcacgaacgg gtccatcgtg tcgtagtatt
     1021 tcttcggcag gtcgccgcca gtcggaaacg gggatttccg cgacagggga atgtgcgaat
     1081 gttcaggagc ccaaaccgat tcgaagcccc gctgttcgag cgcctgccca agctcgcccg
     1141 gggccatgga gtagtcggtg aagaacatcg agacgccgaa tttcagcata ttgttttttc
     1201 ctcctgattg tattgaccga tgatggccgg tttggccgcg ttgtccagac caaccggacc
     1261 ggttcgccat gcccggtagc cgtcgccgga ttggcacgct aaacaaagcg gctctgccac
     1321 acgaggaaag caactttgtc cgaaccggcg cgtgaccctg cctccccttc cgcgcctgaa
     1381 aagagcacag ccgccaagtc cacccccgtc aagatcactg atgtcgccgc cgcggcaggg
     1441 gtcgcgccaa tgaccgtctc acgcgtgatc aacacgccgg atcgggtttc gccggaaacc
     1501 actgctcggg ttaaagaggc gatcgaccgc ctgggctacg ttccgaacct gatcgccggc
     1561 ggcctttcct cgcgcaagtc ccggatggtg gcggcgatcg tgccgaccat cgcccatccg
     1621 atgttcgccg gcctggtgca ggcgttctcc gcctcgatgc gccattccgg ctatcaggtc
     1681 atgctgtcga tcagcgggta cggggaaacc gacgacgaag cattgttccg ggcgctgctg
     1741 gggcggcggc cggacgcg
//
LOCUS       DASWQN010000417         2799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_172106, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000417 DASWQN010000000
VERSION     DASWQN010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172106"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VGC82_06830"
     CDS             <1..198
                     /locus_tag="VGC82_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423023.1"
                     /translation="DALANAYRSRRDAWPDMRLLGCIDQALDAAAEAETGRADALLGL
                     VGIRRGLFPSAPPYGQQAAGA"
     gene            195..407
                     /locus_tag="VGC82_06835"
     CDS             195..407
                     /locus_tag="VGC82_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEY0423024.1"
                     /translation="MRSEFDLFGVFVPGLLFCGLLACLLNAVLRRTLAWIGFYRIVWH
                     RALFDLALFFLVLGAVVWLTAAAGWQ"
     gene            404..1276
                     /locus_tag="VGC82_06840"
     CDS             404..1276
                     /locus_tag="VGC82_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY0423025.1"
                     /translation="MRRFPLAARVAVTLAAVAVAVLIGWRLWVYYEEAPWTRDARVGA
                     DIVGVTPDVSGLVGEVRVKDNQIVHRGDLLFVVDPARFNLALRQTEAVVASRLATLQQ
                     TVRDLNRANSLTSGEISRQQVEQAQAAEQGAAANYQQAIADHDVAKLNLDRTQVSAPV
                     NGIITNFDLRPGDYVTAGHPVTALIDTDSLRIDAYFEETRLPRIHPGDRVSIRLMGES
                     QRLTGQVESIAGGIEDRERQSGANLLANVNPTFSWVRLAQRIPVRITLIDVPAGVRVI
                     PGRTATVDVIGPGS"
     gene            1318..2265
                     /gene="hemC"
                     /locus_tag="VGC82_06845"
     CDS             1318..2265
                     /gene="hemC"
                     /locus_tag="VGC82_06845"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY0423026.1"
                     /translation="MTQAGQPLLRAGTRGSPLALVQTRTFLRLFAAVPGTGEGQEAVI
                     ATTGDAVQDRPLADIGGKGLFAKEIHEALLDRRVDCAVHSLKDLETELPLGIVLGCTL
                     EREDARDVLILGDSCGKPDEADPLAALPHAALVGTSSVRRQAQLLHARPDLRVASIRG
                     NVQTRLRKLREGECQASMLALAGLRRLGMDIQGAAVLDPDTMVPSAAQGIVGITVRAD
                     NEWLLELLARIEDPVSRSVATAERAMLAALDGSCRTPIGAHARLLPDGGLTLTGLVAR
                     ADGSFLLKRTLTGGVKDAARIGADLGASLRADSPADLFP"
     gene            2325..2558
                     /locus_tag="VGC82_06850"
     CDS             2325..2558
                     /locus_tag="VGC82_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423027.1"
                     /translation="MKVLIVGTALAALLAAGPVQAKGCIKGAVVGGLAGHLAHHGVLG
                     AAGGCAVGHHMASQKEKQADQQQMQQQQQTQYR"
BASE COUNT          458 a          867 c          970 g          504 t
ORIGIN      
        1 gacgccctgg cgaacgccta ccgctccagg cgggatgcat ggccggacat gcggctgctg
       61 ggctgcatcg accaggcact cgatgcggcg gccgaagcgg agaccggacg ggccgacgcg
      121 ttactgggat tggtaggcat ccggcgaggc ctgttcccca gcgctccacc atacggacag
      181 caggcagccg gcgcatgagg agcgaattcg acctgttcgg cgttttcgtt cccgggctgc
      241 tgttctgcgg tttgctggcg tgcctgctga acgccgtgct gcggcggacg ttggcatgga
      301 tcgggttcta ccggatcgta tggcaccggg cgttgttcga cctcgcgctg ttctttctcg
      361 tgctgggcgc ggtcgtatgg ctcaccgctg cggcggggtg gcaatgagac ggtttccgct
      421 cgctgcccgg gtggcggtca cgctggccgc ggtggcggtc gctgtgctga tcggctggcg
      481 gctttgggtg tattacgagg aagctccctg gacccgcgat gcccgcgtcg gcgccgacat
      541 cgtcggcgtt accccggacg tgtccggcct ggtcggcgaa gtgcgggtca aggataacca
      601 gatcgtgcat cgcggcgatc tgttgtttgt cgtggacccg gcccgtttca acctggcgct
      661 gcggcaaacc gaggccgttg tcgccagccg cctcgccacc ctgcagcaga ccgtgcgcga
      721 cctcaatcgg gctaacagcc tgacgtcggg agagatcagc cgccagcagg tggaacaggc
      781 gcaggcggcc gaacaaggcg ccgccgcgaa ctatcaacag gccatcgccg accatgacgt
      841 ggcaaaactg aatctggatc gcacgcaggt cagcgcgccg gtgaacggca tcatcaccaa
      901 cttcgacctg cgtcccggcg attatgtgac cgccggtcac ccggtgacgg cgctgatcga
      961 taccgactcg ctgcggatcg atgcatattt tgaggaaaca cggctgcccc ggatccaccc
     1021 tggcgatcgg gtaagcattc gcctgatggg cgaatcgcag cgtctgaccg ggcaggtcga
     1081 aagcattgcc ggcggcatcg aggatcgcga gcggcagtcg ggcgccaatc tcctggccaa
     1141 cgtcaacccg accttcagtt gggtgcggct ggcgcagcgc atcccggtcc gcatcacgct
     1201 gatcgacgtg ccggccggcg tgcgggtgat cccgggacga acggccacgg tggacgtcat
     1261 cggtccggga tcataaagtc attcgtccgt tcggcgagcg agtgctatca accgtttatg
     1321 acccaggctg gacaaccatt gcttcgcgcc ggcacgcgcg gctccccttt ggcgctggtg
     1381 caaacgcgta cgtttttgcg cctgttcgcc gctgtccccg gtacgggaga gggccaggaa
     1441 gccgtgatcg ccaccaccgg cgatgccgtg caggaccgtc cgctggcgga tatcggcggc
     1501 aagggcctgt tcgcgaagga aatccacgag gcgctgttgg atcgccgcgt cgattgcgcc
     1561 gtccatagtc tgaaggatct ggaaaccgag cttccgctgg ggatcgtcct gggatgcacc
     1621 ctggagcggg aggatgctcg ggatgtgctg atccttggcg acagctgcgg caaaccggac
     1681 gaagcggacc cgctggcggc gctgccgcac gcggcgctgg tcggaacctc gtcagtccgg
     1741 cggcaggcgc agttgctgca tgcccgcccc gaccttcgcg ttgcatcgat ccgcggcaac
     1801 gtgcaaaccc ggctgcgaaa gctgcgcgag ggagagtgcc aggccagcat gctggcgttg
     1861 gccgggctgc gccgcctggg catggacatt caaggtgccg ctgtcctgga cccggacacc
     1921 atggtgccgt ccgccgcaca gggcattgtc ggcatcaccg tgcgggccga caatgaatgg
     1981 ctcctggaac tgctcgcgcg aatcgaagac ccggtatctc gttccgttgc gaccgccgaa
     2041 cgggcgatgc tggcagcgct ggacggatca tgccgcacgc cgatcggggc gcatgcgagg
     2101 ttgctgccgg atggcggact gacgctgacg gggctggtcg ctcgggccga tgggtcgttc
     2161 ctgctgaaac ggactttgac cggtggcgtg aaggacgcgg cgcgcatcgg tgcggatttg
     2221 ggcgccagcc ttcgagccga ctcacccgcc gatctgttcc cctaaccgga accaacggtc
     2281 gcgttcaagc gtttcactgc agagacaatg caggagcgtt cgaaatgaag gttttaatcg
     2341 tggggactgc tctcgcggca cttttggcgg cgggtccggt ccaggccaaa ggctgcatca
     2401 aaggcgcggt cgttggcggt ttggccggac acctggctca tcatggggtg ctcggggccg
     2461 cgggaggctg cgcggtcggc catcacatgg cgtctcagaa ggaaaagcag gccgaccagc
     2521 agcagatgca gcagcaacaa cagacacagt accggtaggt tcgcgcgtca ccggtgggtt
     2581 cgcgcgtccg cagcgggggc gtggtatttg tggtatttcc ggacgttgct gtcggggatc
     2641 cttcggccgt tgcgctaaag cttctggcgc gggcaacggg acggccccgg cgctttactc
     2701 gtgaacaagc acccagtcgt tgataaagct tgccaggccg ttagcatgaa agtcccgccg
     2761 cagtgctgct tcgtcgtagt cgtcccgaat ccagtccag
//
LOCUS       DASWQN010000418         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2019434, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000418 DASWQN010000000
VERSION     DASWQN010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2019434"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1136)
                     /locus_tag="VGC82_06855"
     CDS             complement(60..1136)
                     /locus_tag="VGC82_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0423028.1"
                     /translation="MDTVMPDRPPSSESFQRPDPLRRGLLLGGGIAAGMTLAGAASAQ
                     SSTDNEPRVLNGQPVPEAAADKSAGPIRPGRGSALTGKVAVVTGAARGIGRAIAVEFA
                     ANGADVVAIDIAGPVSPASDAKPAKPEDLAETVRQIQAYGRKADSIRADIRDIAALRA
                     AADKIERDHGKIDIVVADAAIQRWKPLLEMADSDWNDVIENNLNGTANTIRAFAPKMV
                     ARQHGRIIVLSSMQGKHGTKDGASYSASKWGILGLMKSASMELGQHNITVNALIPGLV
                     DTPLTRYETRLRESMSENGQKPPEHPTPQQAWDNRAPTVPLKVGWLQPDDISPAAVFL
                     ASDAAAMVTGAEYEVTGGDSAKDI"
BASE COUNT          255 a          483 c          474 g          289 t
ORIGIN      
        1 gactatgtgc tgacggaaat gttcttcgcg cagtagcggg cggtagagcc aacggcctgt
       61 cagatatcct tcgcactgtc gccgcccgtt acttcgtatt ctgccccggt caccatcgcc
      121 gccgcatccg acgccaggaa aaccgctgcc ggagaaatat cgtccggctg caaccagcct
      181 accttcagcg gcacagtcgg cgcgcggttg tcccaggcct gctgaggcgt cggatgttcc
      241 ggcggcttct ggccgttctc ggacatgctt tcgcgcaggc gcgtctcgta ccgggtcagc
      301 ggcgtatcga ccaggccggg gatcagtgca ttgacggtga tgttgtgttg ccccagttcc
      361 atcgaagcgg acttcatcag ccccaggatt ccccatttcg acgccgagta gctggctccg
      421 tccttggtgc cgtgctttcc ctgcatcgag gacagcacga tgatccggcc gtgctgccgg
      481 gcgaccatct tcggcgcgaa ggcgcgaatg gtgttggcgg tgccgttcag gttgttctcg
      541 ataacgtcgt tccagtcgga gtccgccatc tccagcagcg gcttccagcg ctggatggcc
      601 gcgtcggcga ccacgatgtc gatcttgcca tggtcacgct cgatcttgtc agcggcagcg
      661 cgcagagcgg cgatgtcgcg gatgtcggcg cggatcgagt ccgccttgcg gccataggcc
      721 tggatctggc gcacggtttc cgcgagatcc tcgggcttgg ccggcttcgc atccgaggcc
      781 gggctgaccg gcccagcgat gtcgatcgcc accacgtctg ccccgttcgc ggcaaattcc
      841 accgcgatgg cccggccaat gccgcgggcg gcacccgtca ccacggcaac cttgccggtg
      901 agcgccgaac cacgacccgg tcggatcggg ccagccgact tgtccgccgc cgcctccgga
      961 acgggttggc cgttcagaac acgcggctca ttgtctgttg acgattgcgc cgaggcagca
     1021 cccgccaggg tcatgccggc ggcaatgcct ccgccgagaa gcaatccgcg gcgaagcggg
     1081 tcaggtcgtt ggaacgattc ggatgaaggc gggcggtcgg gcatgacggt gtccattacg
     1141 aggtccagcc atgccaacgg agaagcaggg ccggcgttgc ggttcacggt ccgaacctga
     1201 cagtgctgtg accacaattc gtgccgagca tcgtttcgca tgcttcatgc gtgacgctcg
     1261 accggaccat gccgcgccag cggaggtgca cggcgcggcg gctgaccgat gcaagaagcg
     1321 gaaggcgcaa gcgcgcgcgc ccgagatact gcttctatcg taatataagc aggctctctg
     1381 aagtccggct cagttccgcc aatattatgg ttcacctttc gcaaatcgga atccgcgaag
     1441 aaatactttg agctcttcgt ctagaacatc agacaggtta gcctcatcat acggccccgt
     1501 g
//
LOCUS       DASWQN010000419         8268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1227891, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000419 DASWQN010000000
VERSION     DASWQN010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227891"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VGC82_06860"
     CDS             complement(<1..1150)
                     /locus_tag="VGC82_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEY0423029.1"
                     /translation="MTDAPQTPFLTTRQGHPVGDNQSLRSVGERGPATLENYQFIEKI
                     THFDRERIPERVVHARGTAAHGYFEAYGKIGSEPATKYTRAKVLTQAGTRTPLFVRFS
                     TVIGSKDSPETARDPRGFAVKFYTEDGNWDLVGNNLRIFFIRDAIKFPDMIHAFKPDP
                     VTNRQEAWRFYDFVQHHPEALHMVTWVKSPWGIPANYRHMEGSSVNTYKLVNAEGQAV
                     LAKFSWVPKQGVKNLTTQQAAEIQAKDVGHATRDLYGAIDRGDFPEWEFCVQVMADSE
                     HPELTFDPLDDTKRWPEDQFPLLPVGRMVLNRNPDNFFAETEQSAFGTGVLVDGIDFS
                     DDKMLQGRTLSYSDTQRYRVGANYLQLPVNAPKVKARTNQRDGQMTYQV"
     gene            complement(1323..2669)
                     /locus_tag="VGC82_06865"
     CDS             complement(1323..2669)
                     /locus_tag="VGC82_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423030.1"
                     /translation="MEEAPKAGNLTQAISELERQTIARVARRLLPLLMACYFIAYLDR
                     VNVGFASLTMNKALGFTSAVYGFGGGIFFLGYFIFEVPSNVLLSKVGARVWIARILIT
                     WGIISACTAFIVGPISFYSVRFLLGIAEAGFFPGIILYLTWWFPSYYRSRIVGVFMAA
                     IPLSNILGSLVSGALLGLDGWLGFAGWQWLFLLEAAPAVVLGVAFWLYMTDWPSEAHW
                     LTPEQREWLITRLDSERMQRETIRHYSLRQALLDKRVLLLSLVYFGGTFSGYGIVLFQ
                     PQIVQRLSSGFGMTGVINAIPYVFAAGAMVLWGRHSDSTGERPRHVAIAYSVGAIGLI
                     ATALMTDPLLTMVTLVIAAMGQSSTGPTFWSLPTAMLSGTAAAGGIALINALGNLGGF
                     FGPYLFGLIKDASGGSFTFGLMALALGPILSATIVLALGHDRRLEHIPTRQTVTGR"
     gene            2986..3824
                     /locus_tag="VGC82_06870"
                     /pseudo
     CDS             2986..3824
                     /locus_tag="VGC82_06870"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            4012..5265
                     /locus_tag="VGC82_06875"
     CDS             4012..5265
                     /locus_tag="VGC82_06875"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY0423031.1"
                     /translation="MKRPTRVLHVAATLAGLLLANTTLAQAPSSPTGAPTAPTPPDRQ
                     SALYGRPESPDAAKLAPVTPPPIPTPAARLPVAKLHVPEGFKVEVFASGVANARSMRV
                     GDQGTVFVGSRLLDKVYAITPVNGANVVKVIATGLHRPNGVAFHNGSLYVAEVSKIWR
                     YDNIEANLDHPPKPVLIYDDLPTDEAHGWKFIGIGPDNKLYVPIGSPGTITMPPPTHA
                     QIRRMDLDGKNVETVALGVRNSVGFDWNPVNGDLYFSNNGRDWVSEDIPEDTLHRVPA
                     GTKTALNFGFPYCHQGNFTDPEYGWGHSCSEFDKPVALMGPHVATLGMRFYTGDMFPS
                     SYKNVIFLARHGSWNRTKKAGGDVVAIKLKEDGSVASVEPFLTGFLENNNYNGRPVDV
                     QMLKDGSLLVSDDWNGAVWRVSYSH"
     gene            5278..5898
                     /locus_tag="VGC82_06880"
     CDS             5278..5898
                     /locus_tag="VGC82_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0423032.1"
                     /translation="MHGNLFAGCLTTLGRGFVRVVTLLLVSSTMVANACAQTVEERLA
                     ACGGCHGASGVSGMENVPSLGGQPSDYVLIQLYLFREKQRQIEVMNAMTQGLTDDDLR
                     TLADTMSRLAPPPPAGEAPNAANRERALDLVARYRCGSCHNADFSGHDQIPRLAGQRE
                     DYLLKSLREYKSNARAGYDPAMNEVAQEVKETDIPTLAHYLSGFRP"
     gene            6025..7005
                     /locus_tag="VGC82_06885"
     CDS             6025..7005
                     /locus_tag="VGC82_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423033.1"
                     /translation="MTQTTPEICQLAHAGFRMLGEPVFRLSEGTKVPSMVVRLESHEA
                     VLPLRSVAREFGVTPESSDGQMLILIEQALDYVVSLRLGDKLPSELNGGAASWEPTEQ
                     DRRTAASRVRHNLVRCVFARVGRTAAITGGGATGWEEEPDNRTLLLEAIGGAAGLIEG
                     ADEGEITGRLAALNEELACIECMRRTLIRNINGPRDKLLRIDVTQVPTSRQDTVKQVQ
                     ALTRRGLTEITNRFDEVDLRLDEILAMLSDLPAAVAWLRGQRDWLFRTNHAWGPVFAD
                     WASAPSHFDDFLWKVVERTYLFLAPRFMSFQEWKVRDARRKADATRVKVW"
     gene            complement(7076..8047)
                     /locus_tag="VGC82_06890"
     CDS             complement(7076..8047)
                     /locus_tag="VGC82_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY0423034.1"
                     /translation="MKAVRVIRHGKPSVLVVSDIGTPSPSESEALVKVLGASVNPSDV
                     KNVQGLMEQTTLPRTPGRDFAGVVEAGPASWIGAEVWGTGGDTGFTRDGSHAEYILVP
                     VASLRRKPEALDFDRAASVGVNFITAWKGLVEGAGLRSGETALVIAASGGVGNAAVQI
                     AGRIGARVIGTDRRPPRSGAAIARTGTSLVVDAADIPAAVRAATGGHGADVVLDCVGG
                     VMFRHALDCLARRGRLVEMSVTGAREVTFNLADFYHNENRLIGIDTLKSDLTDSARVL
                     ERLHAGFEAGDYQGTPITRRFPLRDAVAAYEAVAAGEQGRVVLRPWE"
BASE COUNT         1548 a         2569 c         2578 g         1573 t
ORIGIN      
        1 ccacctggta cgtcatctgc ccatcgcgtt ggttggtgcg tgctttcacc ttaggcgcgt
       61 tgaccggtag ttgcagataa ttcgcgccga cccggtatcg ttgcgtgtcc gagtacgaga
      121 gcgtccgacc ctgcagcatc ttgtcatccg agaagtcgat gccgtccacc aggacacccg
      181 ttccgaacgc tgactgctcg gtctcggcaa aaaaattgtc cggattgcgg ttcagcacca
      241 tgcgtccgac cggcagcagc gggaactggt cctccggcca gcgcttcgtg tcgtccaacg
      301 ggtcgaaagt gagctcagga tgctcgctgt ccgccatcac ctgcacgcag aattcccact
      361 ccgggaagtc gccacggtcg atggcgccgt acaggtcccg ggtcgcgtgc ccgacatcct
      421 tcgcctggat ctcggccgcc tgctgcgtgg tcaggttctt cacgccctgc ttgggaaccc
      481 aggagaactt ggccagcacc gcttgtcctt cggcgttcac cagcttgtag gtgttgacgc
      541 ttgagccctc catgtggcgg taattggctg ggatacccca ggggcttttc acccaggtga
      601 ccatgtgcag cgcttccgga tgatgctgca cgaaatcata gaagcgccaa gcctcctggc
      661 gattggtcac cggatcgggc ttgaaggcgt ggatcatgtc ggggaacttg atggcatcgc
      721 gtatgaagaa gatgcgcagg ttgttgccaa caagatccca gttgccgtcc tcggtgtaga
      781 acttcacggc gaagccacgc ggatcgcgcg ccgtctcggg gctgtccttg ctgccgatca
      841 cggtggaaaa gcgcacgaag agcggtgtcc gtgtgcccgc ttgcgtcagc accttggcgc
      901 gagtgtactt ggttgccggc tcgctgccga ttttgccata ggcttcaaaa taaccatgtg
      961 ccgcggtgcc acgcgcatgc acgacgcgct ccgggatgcg ctcacgatcg aaatgggtga
     1021 tcttctcgat gaactggtag ttttccagcg tggccgggcc acgttctcct acgctgcgca
     1081 ggctctggtt gtcgccaacc ggatgtcctt gccgggtggt gaggaatggt gtctgcggtg
     1141 cgtcagtcat atcaggctcc ctcgaacaac gcttcgtcag ggtctgaaac ggaggcgtgc
     1201 cgtcgcggtt gcgctgctac cgaggcggcg actgcctgaa tttttcgggt tgcggtttcg
     1261 gctttgtatc aggcgttccc gccgtcaaac cgcaaactgt atatcgctga cgcttgctcc
     1321 gttcatctcc ccgtaacggt ttgacgcgtc ggaatgtgct ccagccgacg gtcgtggccc
     1381 aacgcgagca cgattgtcgc cgataggatc gggccaagcg cgagcgccat cagcccgaac
     1441 gtgaaactgc caccggacgc gtccttgata agcccgaaca gataggggcc aaagaatccg
     1501 cccaagttgc ccaacgcgtt gatcagcgcg atcccgcctg ccgcggccgt tcccgacagc
     1561 atcgccgtcg gcaggctcca gaaggttggg ccggtggaag attgtcccat cgcggcaatt
     1621 accaaggtca ccatggtcag cagcgggtcg gtcatcagcg cggtggcgat cagcccgatc
     1681 gccccaaccg aatatgcgat ggcgacatgc cgtggccgct ctccggtgct gtcggaatgg
     1741 cggccccaga gcaccattgc accggcggcg aaaacgtagg gaatggcgtt gatcacgccg
     1801 gtcatgccga atccggagga cagtcgttgt acgatctgcg gctggaacaa cacgattccg
     1861 tatccagaga acgtgccgcc aaaatagacc aggctgagca gcagcacgcg cttgtcgagc
     1921 aacgcctgcc tcaggctgta gtggcggatc gtctcacgct gcatccgttc ggaatccagg
     1981 cgcgtgatca accactcgcg ttgttcaggc gtcagccagt gcgcctcgga cggccagtcc
     2041 gtcatataga gccagaatgc cacgccgaga acgacggccg gcgccgcctc cagaaggaac
     2101 agccattgcc agccggcgaa gcccagccag ccatcaagac cgagcagcgc accggaaacg
     2161 agcgaaccca ggatgttgga gagcgggatc gccgccatga acaccccgac gatgcgggag
     2221 cggtagtacg acgggaacca ccaggtcagg taaaggatga tcccggggaa aaagcccgct
     2281 tcggcaatcc cgagcaaaaa gcggacggag tagaacgata tggggccgac gatgaaggcg
     2341 gtgcaggcag agatgatgcc ccaggtaatc agaatccgcg cgatccacac ccgcgcgcca
     2401 accttgctga gcagcacgtt gctcggcacc tcgaagatga aatacccaag gaaaaagatg
     2461 ccgccgccga acccatagac cgccgatgtg aaccccagtg ccttgttcat ggtgagcgag
     2521 gcgaagccga cgttcacgcg gtccagatag gcaatgaaat agcaagccat cagcagcggc
     2581 agcagcctgc gggcgacgcg ggcgatcgtc tggcgctcga gctccgagat cgcctgcgtc
     2641 aggttgccgg ctttcggtgc ttcctccatt ttttgacccc tgtttgttga cgttgacgct
     2701 aaagcacttt cagcctgact gtagaggtta aaatgctcta tcctcttgtt tgatcgcgtg
     2761 attcacccgc ctcgggcgtc ccgcccggcg tgatcaagct ctagcctgca tcccgcggcg
     2821 gaacaagatt caggaccggc cggtacggct catcccgcgc gtgaccggct gacgccgtcg
     2881 tggatgtaag gtagtctcga acgaaatccc gtttggcaaa taggaggcgc gatcatggac
     2941 gccggcgacc tacggatgtt cgaagcggtc gcgcgcctcg gcagcatgaa ccgcgccgcc
     3001 ggcgaactga atacggtcca gtcaaacgtc accggtcgca tccgggcgct ggaacttgaa
     3061 ctgggagtgg agctgttcaa gcggcatgcg cgaggcgtgt cgccgactgc cgcagcgcag
     3121 cgattgttgc cgtacgcgct gcggatacgg cttatgctgg ataacgcccg caaagccgcg
     3181 cgggacgatg gtgtgccggc aggtccgctc gtggttgggt cgctggaaac caccgcggcc
     3241 gttcgtttgg cgccgttgct ggcggatttt accgccgccc atccgcatgt ggacctgtct
     3301 ttgcgtactg gcacgagctg cgaactgatc gacgatgtgc ttttcgatcg cgtcgagggc
     3361 gcctttgtct gtggcccggt cgatcatccc gagctaacca gcgaggcgat gttccaggag
     3421 gaactggtcg ccctgacggc accgggcgcg acaacttttg aggcggtgat cggggcaggg
     3481 ggctttcgga ttgtcgtcct gcgagccggc tgttcctacc gtctccggct cggggctctt
     3541 ttggcccgtc gtggttccgt gggcgcccga tcatggaatt cggcacgttg gaggcaatcc
     3601 tggcctgtgt cggtgcgggt cttggggtaa ccctgctgcc gcgcgccttg gtggaaaatg
     3661 cctggcgcgg cggccggatc ggcgtccacg cattgccgcc ggaggacgct ttcgttgaaa
     3721 cggtcttcgt gcggcggcgt gacctctatg cgtcaagcgc gatggcagca tttctcgaag
     3781 ccgtccgacc ggttcggccc tcggcgcggg cggcggcgga gtaatctgtt cagcagatcg
     3841 ctgtcccccg tgagatcgct gtcgcccgtg ccggtcaagc ggcaccgtgc cgcaggaatc
     3901 accggtcgca tcgttctggt cgctagtacc ggctgttgtt agtgcccgct ggacagaccg
     3961 tccggttaaa tccaggatat ggatcataaa aataagcgag ggtgcgccgg gatgaaacgt
     4021 ccgacacgag ttttgcatgt cgcggcaaca ttggccgggc tgctgttggc taacaccacc
     4081 ttggctcaag cgccatcgag cccgacaggc gctccaaccg ctccgacgcc acctgatcgg
     4141 cagtccgcgc tttatggcag acccgaaagc cccgacgcgg cgaagcttgc gccggtaacc
     4201 ccgccgccga tcccgacacc ggccgcgcgg ttgccggtcg cgaaactgca cgttcccgaa
     4261 ggcttcaaag tcgaggtatt tgccagcggc gttgccaatg cccggtcgat gcgggtgggc
     4321 gaccaaggca ccgtgttcgt cggctcccgg ctgctggaca aggtttacgc catcactccg
     4381 gtcaacgggg cgaacgtggt caaagtgatc gcaaccggtc tgcatcgccc gaacggcgtc
     4441 gcctttcaca acggctcgct ttacgttgcg gaggtgtcga agatttggcg ctacgacaac
     4501 atcgaggcca acctcgacca tccacccaag cccgttctga tctatgacga tttgccgaca
     4561 gacgaggctc atggctggaa gttcattggc atagggccag acaacaagtt gtacgtgccg
     4621 atcggctcgc ctggcaccat caccatgccg ccgcctactc acgcgcagat ccgccgcatg
     4681 gacctcgacg gcaagaacgt cgagacggtg gcgctgggtg tgcgcaactc ggtcggcttc
     4741 gactggaatc cggtcaatgg ggacctgtat ttcagcaaca acggccgtga ctgggtatcc
     4801 gaagacatcc ctgaggacac gctgcaccgc gtccccgctg gaaccaagac tgcgttgaat
     4861 ttcggctttc cctactgcca ccagggcaac ttcaccgacc cggaatatgg ctggggccat
     4921 tcctgcagcg agtttgacaa gcccgtcgcc ctgatgggtc ctcacgtagc gaccctgggg
     4981 atgcgctttt acacgggcga catgttcccc agcagctaca agaatgtcat tttcctcgcc
     5041 cgacacggat cgtggaaccg gacgaagaaa gctggtggcg acgtggtcgc catcaagctg
     5101 aaagaagacg gttcggtcgc ctcggtcgag ccgttcctga ccgggttcct ggagaacaat
     5161 aactacaatg gacgtccagt cgatgtgcag atgttgaagg acggttcgct tctggtgtcg
     5221 gacgactgga acggcgccgt gtggcgcgtc agttacagcc actaaagctt tgacccgatg
     5281 cacgggaacc tgttcgccgg ctgcttaacg acgctcggcc gtggctttgt gcgggtggtc
     5341 accctgttgc tcgtttcatc cactatggtg gctaacgcct gcgcccagac ggtcgaggag
     5401 cgcctggcgg cctgcggcgg ctgccatggc gcctccggcg tatccgggat ggaaaacgtt
     5461 ccttcccttg gcggccaacc gtcggattac gtgctgatcc aactctacct gtttcgcgaa
     5521 aagcagcgcc agatagaggt gatgaacgca atgacccagg gcttgaccga cgacgatttg
     5581 cgcaccctgg cggacaccat gtccaggctt gccccgccac ctccagcggg tgaggcgccg
     5641 aatgcagcca accgcgagcg cgccttggat cttgtggcaa gatatcgctg cgggtcgtgt
     5701 cacaacgccg acttctccgg ccatgaccag attccccgct tggccggtca gcgcgaggat
     5761 tatctgctca agtcgttgcg agaatacaaa tccaatgctc gcgcaggcta cgatccggcg
     5821 atgaacgagg tggcccagga ggtcaaggag actgatattc cgacgctggc gcattacctc
     5881 agcgggtttc gtccgtagcg tgtatcgacg acatgcgctg ccgcggcgcg aaggttgcgt
     5941 gaaatcggga ctgggttcac gctttattaa gctttgtctg tcctgattgg tccgtcagca
     6001 tccgcaaacg cccggaccaa tttcatgacc cagactaccc ccgaaatatg ccagttggcc
     6061 cacgcgggtt tccgtatgct gggcgaaccg gtcttccggc tcagcgaagg aacaaaggtt
     6121 ccctcgatgg tggtgcggct tgagagccac gaggcagtgt tgccgcttcg ctccgtcgca
     6181 cgcgagttcg gtgtgacgcc tgagagcagc gatggccaaa tgctgatcct gatcgaacaa
     6241 gcgctggatt acgttgtctc gctgcggctt ggcgacaaac tgccatcgga actcaacggc
     6301 ggcgccgcga gctgggagcc aaccgaacaa gatcgcagga cggccgccag ccgggtgcgg
     6361 cataatcttg tccgatgcgt ctttgcccgc gttggccgca ccgctgcgat cacgggtggc
     6421 ggagccacag gttgggagga ggagccggac aatcgaaccc tgttgcttga agcaatcggt
     6481 ggcgcggcgg gcctgatcga gggtgccgac gaaggcgaaa tcaccggccg gctcgctgcg
     6541 ctgaacgagg aattggcctg catcgagtgt atgcgccgca ccttgatacg caacatcaac
     6601 ggaccgaggg acaaactgct gcgtatcgat gttactcagg ttcccaccag ccgccaggac
     6661 acggtcaaac aggtccaggc gctgactcgc cggggcctga cggagatcac caaccgtttc
     6721 gacgaagttg acctgcgcct ggacgaaatc cttgcgatgc tgagcgacct gcctgccgcg
     6781 gttgcctggc tgcggggcca gcgggactgg ctgtttcgta cgaaccacgc gtgggggccg
     6841 gtttttgccg actgggcgag cgcgccaagt cacttcgacg actttctatg gaaagtcgta
     6901 gaacggacct acctgttcct ggcccctcgg ttcatgtcgt tccaggaatg gaaggtcagg
     6961 gacgctcggc gaaaggcgga tgcgacgcgg gtgaaagtct ggtgattcga acggggccgg
     7021 cggatgttgg ggagatcgcc agatcgttac ttatacgaac gatatcattg gtcaactact
     7081 cccacggccg caacaccacc cggccctgct cccccgccgc gaccgcctca taggccgcga
     7141 cggcatcccg caacgggaat cggcgggtga tcggcgtgcc ctggtagtcg ccggcttcga
     7201 aaccggcgtg caaccgttcc agcacccgcg ccgagtccgt caggtctgat ttcagcgtgt
     7261 cgatgccgat caggcggttt tcgttgtgat agaagtcggc gaggttgaac gtgacctccc
     7321 ttgctccggt cacgctcatc tccaccaacc ggcccctccg ggcgaggcag tccagcgcat
     7381 gccggaacat gacgcccccc acacaatcca gcaccacatc ggcgccgtgg ccgcctgtcg
     7441 ccgcgcgcac ggcggccggg atatcggcgg catccacgac gagcgaagtc ccggtccgcg
     7501 cgatggcggc tcccgaacga ggtggcctgc gatccgtgcc gatgacccga gcacctatgc
     7561 ggccggcgat ttggacggcg gcattgccaa cgccgccgct cgccgcgatc accagcgcgg
     7621 tctcgcccga ccgtaacccg gcgccttcga ccaagccctt ccacgcggtg atgaagttca
     7681 ccccgacgga ggccgcccga tcgaagtcca gtgcctcggg tttgcgccgc aggctggcga
     7741 ctgggacaag gatatactcg gcatgcgatc cgtcgcgggt gaaacccgtg tcgccgccgg
     7801 ttccccatac ctcggcaccg atccaggacg ccggtccggc ttccaccacg ccggcaaagt
     7861 cgcgcccggg tgtcctgggc agggtggttt gctccatcag tccttgcacg ttcttgacgt
     7921 cgctgggatt gaccgacgcg ccaagcacct tgaccaacgc ttcgctttca ctcggtgatg
     7981 gggttccgat atcgctgacc accagtacac ttggcttgcc gtggcggatc actcgaacgg
     8041 ctttcataac atcctccatg gctgcacccc gatcgcttga cgcggcggtc attcaggcga
     8101 cactgcctca accgtgagga gggggaaaca ccacatgagc gacgttgaga tcctggctac
     8161 cggcttcggc ttcccggaag gccccgtcgt ctgcaaggac ggatcaatca ttctgactga
     8221 aattcgtaac aaccagttgt ccaggatcgc gccggacggc aagaccag
//
LOCUS       DASWQN010000420         3036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_568051, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000420 DASWQN010000000
VERSION     DASWQN010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568051"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VGC82_06895"
     CDS             <1..518
                     /locus_tag="VGC82_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423035.1"
                     /translation="GDDGAITATGSGLDVLSPSSSGQSGIRVAARAGPGDADMPNRET
                     SGTAVIRCEKGACLLRPYLDRPGALLARGAPHPAGCNEVSVIVSAEPARGLCPRPWPA
                     LVDRFTVWRNGSAAIWLERGGARIVTDRMERGDRPWVPPPPRPRKTIPNLPPAAVDRV
                     EQPRLVPDVPP"
     gene            540..1277
                     /locus_tag="VGC82_06900"
     CDS             540..1277
                     /locus_tag="VGC82_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEY0423036.1"
                     /translation="MFYHRKETIIPVDVKNPDPRFGAFLLEQFGGATGELTAALQYWV
                     QSFHVTDASIRDMLQDIAIEEFSHLEVVGKLIEQHTSKLDQTDVYAAPLFRVRGVGPH
                     FVDSQGSAWTAAYINEGGNVVRDLRADIAAEAGARQTYEALIHASTDEGTTKALTHLL
                     TREITHAQMFMAALERMGKLDDPYFGTIKPDETVDLVFNLSKGDDHRGPWNMEPLFRY
                     NDDPKPMGGLPPAPVNPDDEKMSLAAK"
     gene            complement(1437..>3036)
                     /gene="groL"
                     /locus_tag="VGC82_06905"
     CDS             complement(1437..>3036)
                     /gene="groL"
                     /locus_tag="VGC82_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423234.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0423037.1"
                     /translation="VDILADAVKVTLGPKGRNVVIDKSFGAPRITKDGVTVAKEIELA
                     DKFENMGAQMVREVASKTNDIAGDGTTTATVLAQSIVREGAKAVAAGMNPMDLKRGID
                     KAVTAVVEELKRRTKKITTPAETAQVGTISANGESEIGKMIAEAMQKVGNEGVITVEE
                     AKGIATELDVVEGMQFDRGYVSPYFITNAEKMIADMDQPYILIHEKKLSGLQPMLPLL
                     ESIVQSGRPLVIIAEDVEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLEDIAIL
                     TGGTVISEDLGIKLENVKLADLGKAKKILIEKENTTIVEGAGKKPDIQGRCGQIRAQI
                     EETTSDYDREKLQERLAKLAGGVAVIRVGGSTEVEVKERKDRVDDSLHATRAAVEEGI
                     VPGGGVALARASLILSKVIADNDDQRFGIEIIRKAIQTPLRQIAENAGEDGAVIAGKT
                     LERDEYNYGFDAQTGEFKDLVGAGIIDPTKVVRTALQDAASVSGLLITTEAMVAERPE
                     KKGAGMPQGGGGMGGMGGMGDMDF"
BASE COUNT          505 a         1021 c          919 g          591 t
ORIGIN      
        1 cgggcgatga cggtgctatt accgcgaccg gctccgggct cgacgtgctg tcgccgtcat
       61 cgtcgggcca gtccggaata cgtgtcgccg ctcgtgccgg accaggtgat gcggacatgc
      121 cgaaccggga gacgagcgga accgcggtga tccgctgcga gaaaggcgcc tgcctgctcc
      181 gtccttatct cgaccggccc ggagcgctgc tggcgcgtgg cgctcctcac ccggcaggct
      241 gcaacgaggt ctcggtgatc gtttccgccg aaccggcgcg tggcctgtgc cctcggccat
      301 ggccggcttt ggtcgatcga ttcaccgtgt ggcgcaacgg cagcgccgca atctggctgg
      361 agcgcggcgg cgcccgcatc gtgaccgacc gcatggagcg gggcgatcgg ccatgggtgc
      421 cgccgccgcc gcggccccga aagaccattc ccaatctacc cccggctgcc gtcgatcgag
      481 tggagcaacc aagactcgtg ccggacgttc caccttaggg accaacaagg gagtagccga
      541 tgttttacca ccgcaaggaa acgatcatcc cggttgatgt gaagaacccg gacccgcgct
      601 tcggcgcatt tctgttagaa caattcggcg gtgcgacagg agaattgacc gccgccctgc
      661 aatactgggt gcagtccttc cacgtcacgg atgcatcgat ccgcgacatg ctgcaggaca
      721 tcgcgatcga ggaattcagc cacctcgagg tcgtcggcaa actgatcgag cagcacacca
      781 gcaagcttga ccagacagat gtctatgcgg cgccgttgtt ccgcgtgcgc ggcgttgggc
      841 ctcatttcgt tgacagccag ggcagcgcct ggactgccgc ctacatcaac gagggcggca
      901 acgtggttcg cgacctgcgt gccgacatcg cagcggaagc cggtgcgcgc cagacctacg
      961 aagcgctgat ccatgccagc acggatgagg gcactacgaa ggcgctgacc catctgctca
     1021 cgcgcgaaat cactcatgcc cagatgttca tggcggcgtt ggagcggatg ggcaagctgg
     1081 acgatcccta tttcggcacc atcaagcccg atgaaaccgt cgatctggtt ttcaatcttt
     1141 caaagggcga cgaccaccgc gggccgtgga acatggaacc gctgttccgc tacaacgacg
     1201 atcccaagcc gatgggcggc ctgcctccgg cgccggtcaa tcccgacgac gaaaagatgt
     1261 cactggctgc aaaatagcgc tgcaccggct cggattttga gcattttcag gctaatgcgc
     1321 agcctggaag tgctccgagc agtcgctctc ccaccaggaa cgacagggcc acaaatgaag
     1381 cgggccgctc ccatccagga gcggcccgct tttcgtcagt cggaagatgg tacggattag
     1441 aaatccatat cgcccatgcc gcccattccg cccatgccgc caccgccctg aggcatgccc
     1501 gcacccttct tctccggacg ctcggccacc atcgcttccg tcgtgatgag caggccggaa
     1561 accgatgccg catcctgcag cgccgtgcgc acgaccttgg tcgggtcaat gatgccagcg
     1621 cctaccaggt ccttgaactc gccggtctgt gcgtcgaagc cgtaattata ctcgtccctc
     1681 tccagcgtct tgccggcgat caccgcgccg tcttcaccgg cgttctcggc gatctggcgc
     1741 agcggggtct ggatcgcctt gcggatgatc tcaatcccga agcgctggtc gtcgttgtcg
     1801 gcaatgacct tggacaggat cagggacgcc cgcgccaggg caacgccgcc gcccggaacg
     1861 attccttcct cgaccgccgc gcgcgtggcg tgcaggctgt cgtccacgcg atccttgcgc
     1921 tctttcactt ccacttcggt gctgccaccg acgcggatga ccgcaacgcc gccggccagc
     1981 ttcgccagac gctcctgcag cttctcgcgg tcgtagtccg aggtggtttc ctcgatctgc
     2041 gcccggatct gaccgcaacg gccctggatg tcgggcttct tgccggcacc ttcgacgatc
     2101 gtggtgtttt ccttctcgat caggatcttc ttggccttgc cgaggtccgc gagcttcacg
     2161 ttctccagct tgatgccaag gtcttcgctg atcacggtgc caccggtcag gatcgcgatg
     2221 tcttccagca tcgccttgcg acgatcgcca aagccaggcg ccttcacggc cgcgaccttc
     2281 aacccgccgc gcagcttgtt taccaccagg gtcgcgagcg cctcgccctc gacgtcttcg
     2341 gcgatgatca ccagcggacg gccagactgc acgatgcttt ccagcagcgg cagcatcggc
     2401 tgcaggccgg acagcttctt ctcgtggatc agaatgtagg gctgatccat gtcggcgatc
     2461 atcttctccg cattcgtaat gaaatacggg gagacgtagc cgcggtcgaa ctgcatgccc
     2521 tcgacgacgt cgagttccgt ggcgatgccc ttggcttcct cgacggtgat gacgccctcg
     2581 ttaccgacct tctgcatcgc ttccgcgatc atcttgccga tctcggattc gccgttcgcg
     2641 gagatcgtgc cgacctgtgc cgtttcggcc ggggtggtga tcttcttggt gcggcgcttc
     2701 agctcttcca cgacggcggt aaccgccttg tcgatgccgc gcttcaggtc catcgggttc
     2761 atcccggcgg ccacggcctt ggcaccctca cgcacgatcg actgcgccag caccgttgcg
     2821 gtggtggtgc cgtcgccggc gatgtcgttg gtcttcgagg ccacttcgcg caccatctgg
     2881 gcgcccatgt tctcgaactt gtcggccagc tcgatctcct tggcgacggt gacgccgtcc
     2941 ttggtgatgc gcggggcgcc gaagctcttg tcgatcacga cgttgcggcc cttggggccg
     3001 agcgtcacct tcaccgcatc ggcgaggatg tcgacg
//
LOCUS       DASWQN010000421         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1491383, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000421 DASWQN010000000
VERSION     DASWQN010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491383"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1163)
                     /locus_tag="VGC82_06910"
     CDS             complement(222..1163)
                     /locus_tag="VGC82_06910"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HEY0423038.1"
                     /translation="MSGVSGGWLDRLCRLTRSRDHVLFAGLTSLVCMVAVGCTPPLKP
                     DPHYVLGKPYQAGQVWFYPRESYDLDETGLAGIVRNNADRLTSDGEVFDQTVLAAAHP
                     TSQLPAIARVTNLDNGREITVRLNDRGSGDPRRLVEITRRAAELLDMPTNGATRVRLR
                     ILPNESHAAADALPGAPSLTMTAAPRGTVEVAELPPPPGVRRGSDRVLHTVPFAPPPE
                     AAPPAPPMRLPETVIQTSPRPGLLIVRLDIFDDYRYAAAQRARMAAAGARIVSIVQGR
                     THRFEVEIGPLPDVARADAVLDQALAAGIPDARIVVE"
     gene            complement(1206..2171)
                     /locus_tag="VGC82_06915"
     CDS             complement(1206..2171)
                     /locus_tag="VGC82_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY0423039.1"
                     /translation="MSTRRYLLASAIPAVLSATAFGSPPESFASFLTGIRAEARRTGI
                     MPATLERAFAGVSANQKVLERDRHQPEFTMTWARYRALVISDKRISDGRQAIAANLSL
                     FQKVEQRYRVSSGVIAGIWGLESSFGTGTGDYRVVEALATLAWDGRRASFFREELMAA
                     LKILDHGDVMPGAMTGSYAGAMGQPQFMPSSYLRYAVDFEGHGRRDIWNSKPDVLASI
                     ANYLARSGWRDGGTWGQPVTVPSGIDAPGRDSKRPLSEWIRSGVRPVSGRWVARADTQ
                     AGLVAPDGAGGETFVVFDNFAAIRRYNPSDFYAIAVGLIGDQVAT"
     gene            complement(2246..2503)
                     /locus_tag="VGC82_06920"
     CDS             complement(2246..2503)
                     /locus_tag="VGC82_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423040.1"
                     /translation="MPLYAVLVRGYFLVAIMGSVFGVVAMVSTLGMALEPPIGGWLFD
                     QFGSYVWRYVGLLGPGLAAVGIALTVRPPRPEVARLTPNMA"
     gene            3079..>3359
                     /locus_tag="VGC82_06925"
     CDS             3079..>3359
                     /locus_tag="VGC82_06925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423041.1"
                     /translation="MTNTRLWLLLAAAFGTFSVGAGFMHAYTVFLVTFIEVFGWTRAQ
                     VSLAYSVSQIVSGASSPLVGWLVDRLGPPRLILVGGVLLSLGLLVSSRAT"
BASE COUNT          628 a         1101 c         1068 g          562 t
ORIGIN      
        1 gtgggcggaa cgggcagacg aacgtttctg ctgggccaag cctggtccgg cgccaaaagc
       61 agaggcggcc accaataagc caagacgacg ggtcaacatc tcgacgcaac ctcccctgcg
      121 agtctcttca tacccaccat acaacacgca ggtgttcttg cgctttattt gataacagga
      181 caggcgcgtc ccacgaagag cccattcggt ctatcatcgc ctcactccac cacgatccgc
      241 gcatccggta tgccggccgc gagcgcctga tccaacaccg cgtccgctcg ggcgacatcc
      301 ggcaacgggc cgatctccac ctcgaagcga tgggtgcggc cttggacgat agagacgatt
      361 cgggcgcccg cggccgccat cctggcgcgc tgcgcagccg cgtaccggta gtcatcgaaa
      421 atatccaggc gtacgatcaa cagcccgggc cgaggcgagg tctggatgac cgtttccgga
      481 agccgcattg gcggagccgg cggcgctgcc tcgggcggtg gcgcgaaggg aacggtatgc
      541 agtacccgat cgctaccccg gcgcacgccc ggaggtggcg gcaattccgc cacctcgacc
      601 gtgccacgcg gagcagcggt catcgtcagc gacggcgcac cgggcaacgc gtcggccgca
      661 gcatgacttt cgtttggcag gatgcgtagc cggacccggg tcgcaccgtt cgtcggcata
      721 tccaacagtt ccgccgcgcg gcgggtgatc tccacgaggc ggcgaggatc gccggacccg
      781 cggtcattca accgcacggt gatttcgcgc ccgttgtcca gattggtgac gcgggcgatc
      841 gcgggaagct ggctggtcgg atgagcggcg gcgaggacgg tctggtcgaa tacctcgcca
      901 tccgaagtca accggtcggc gttgttcctg acgatccccg caaggccggt ttcgtcgaga
      961 tcgtagcttt ccctggggta gaaccagacc tgccccgcct ggtatggctt gcccagcacg
     1021 taatgtgggt caggtttcag cggcggcgtg cagccaaccg cgaccatgca aaccaggctc
     1081 gtcaggccgg cgaacaggac gtgatccctg gatcgcgtca agcggcaaag acgatcaagc
     1141 cagccgcccg agacgccgct cacgccggag ccaggccggc cgtcatcggc atccgcaccg
     1201 gatcgtcatg tcgccacctg atcgccgatc agcccaacgg cgatagcgta gaaatcggat
     1261 ggattgtacc gcctgatcgc cgcgaaattg tcgaacacga cgaaggtttc tcccccggct
     1321 ccatccggcg ccaccaaccc ggcctgggta tcggcccgcg cgacccaacg gccactgaca
     1381 ggacgcacgc cggatcggat ccactccgac agtgggcgct tggagtcccg ccccggtgcg
     1441 tcgatgccgg acggcacggt gacgggctgt ccccaggtcc cgccgtcgcg ccaccccgac
     1501 cgggccagat agttggcgat cgatgccagc acgtccggtt tgctgttcca gatgtcgcgg
     1561 cgcccgtggc cttcgaaatc gacggcgtag cgcaaatagc tggagggcat gaattgcggc
     1621 tgtcccattg caccggcgta ggaaccggtc atcgcgccgg gcatgacgtc gccatgatcg
     1681 aggattttca gcgccgccat cagctcctcg cggaagaagc tggcgcggcg cccatcccac
     1741 gcgagcgtgg ccagcgcctc caccacgcga taatcgccgg tgccggtacc gaagctcgat
     1801 tccagtcccc agatcccggc gataaccccg gaacttacgc ggtatcgctg ctcgaccttc
     1861 tggaacagag aaaggttggc agcgattgcc tgacggccgt cggagatgcg cttgtcgctg
     1921 atcaccaggg cccggtagcg cgcccaggtc atggtgaact ccggctgatg gcggtcgcgc
     1981 tccagtactt tctgattagc cgacacgccg gcgaaggcgc gctccagcgt ggcaggcatg
     2041 atgccggtcc ggcgcgcctc ggcacgaatg ccagtcagga agctggcgaa actttccggt
     2101 ggcgacccaa aggcggtcgc cgacaggact gctggaatgg cgctggcaag aagataacga
     2161 cgagttgaca tggcgcgctt cctgccatgc caacccgcgc cggagcaatc ggaatcgcga
     2221 catccacagc ctggggcgcg gcctgttatg ccatgttcgg ggtcagtcgg gccacctccg
     2281 gtcttggcgg tcgaactgtc agggcgatcc ccacggccgc gaggccgggt ccgagcaagc
     2341 cgacataccg ccagacgtaa ctgccgaact gatcgaacag ccagccgccg atcggcggtt
     2401 ccagcgccat gcccagcgtg gaaaccatgg cgacgacacc gaacaccgaa cccatgatcg
     2461 caacgaggaa ataccccctc accaggaccg cgtacagcgg catcaccagg acaggccgaa
     2521 caccgccgca acggcattga agctccagcg attggtggtg agctggatca catcggacgg
     2581 tctgtcttcc gcctaccttg ttcggcaaca ggtttccttc agtcgcccac ggcaggaatg
     2641 agacctggac tggcgcaatt cgccgcaacc caggtgagac accgaaatga aaccgcacct
     2701 tcatcctttc gaacccgatc cgtcctggta cgagaggtat tggcttgaag cgacggacaa
     2761 accacggaaa gtgaggtcca ggcggcttcc aatggtcatc gcggcggtct ttggcgaatg
     2821 gctgcgcgct catatggcgg cggcggcaca ggcccggcgg caccggataa gcctggatga
     2881 gatctgagtg atcctcaaag gcttgtaagg aatagcgcca agcgtgccac ggctcggata
     2941 acgcatcaaa ggcggccagc ggcgcggtag cagcgcggat ttcgataagt gccaccgagt
     3001 ctcaccagga atgcgatccc ggggctgggc aatgcggcct ccgccagaca tgtttgccgg
     3061 atgtccgata ccaagcccat gaccaatact cgcctgtggc tgctgctggc cgccgcattc
     3121 ggcacgtttt ccgtcggtgc cggcttcatg catgcctaca ccgtcttcct ggtcacgttc
     3181 atcgaggtat tcggctggac gcgcgcacag gtctcgctgg cctattcggt gtcgcagatc
     3241 gtatcgggtg ccagttcgcc gctggtcggc tggctggtgg accggctggg accgcctcgg
     3301 ttgatcctgg tgggtggggt gctgctgtcc ctgggcttgc tggtcagttc tcgtgcgac
//
LOCUS       DASWQN010000422         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1755919, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000422 DASWQN010000000
VERSION     DASWQN010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755919"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VGC82_06930"
     CDS             complement(<1..536)
                     /locus_tag="VGC82_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY0423042.1"
                     /translation="MSADPFAAPRGSGGRAPDVMRVGGWVPMREYPETEEVDFVVIGT
                     GAGGGPLIARLAEQGFSVIGFDAGPWFRPLEDFASDETEQNKLYWTDPRVIDGENPII
                     MGGKNSGKAVGGSTVHFAMVSLRFRPEWFKSRSLLGYGADWPLDWREMWHYYGEAEQA
                     LKIAGPVTYPWGPKRPRYP"
     gene            complement(526..1173)
                     /locus_tag="VGC82_06935"
     CDS             complement(526..1173)
                     /locus_tag="VGC82_06935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY0423043.1"
                     /translation="MTDNGRYPGYDVLAKRDTMSWNAVTRQVIDRRLAVPRQPQFFTP
                     TEWTTLVALCARILPQPGNRPAVPLAAYVDQRMVNGAGDGYRFADMPDERESWRRGLA
                     ALDAEARARHGESFHAIPTSDQDTLIGQMQRDELHDPAWGDMPRKTFFAHRVLPDITH
                     AYYAHPTAWNEIGFGGPASPRGYVRMALDRRDPWEAAEAHPGEEAASARKNARVG"
     gene            complement(1166..2305)
                     /locus_tag="VGC82_06940"
     CDS             complement(1166..2305)
                     /locus_tag="VGC82_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423044.1"
                     /translation="MVESRTAATTVPVVAVGLGLLAMVARRGEQLHSTAFPSPARNGL
                     MTKSRFEPSELPRQLHVARRPTVDDLDRSCTASRRWRAFAHHDDIRERTQRGYPEADT
                     NSSAAPAVRAARRLNRAAGVLATSVLADSAVEHYRGSFHNPAMFAPLITAALSLGISV
                     HGVADHRHGAHRLRDGVYALAGATGLVGTAFHIFNISRKPGGFTWQNLFYSAPIGAPA
                     ALVLSGATGYLAERVRDNAPATLPTIGGLQAGRVVAAATGIGILGTVAEAGLLHFRGA
                     YHNPFMYAPVTIPPVTAALLGGVAVGAARTPRPLTRWWLRLTAALGFAGAGFHAFGVA
                     RNMGGWRNWRQNVLNGPPIPAPPSFTGLALAGLAALGLLEDHPDD"
     gene            complement(2309..>2945)
                     /locus_tag="VGC82_06945"
     CDS             complement(2309..>2945)
                     /locus_tag="VGC82_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY0423045.1"
                     /translation="DGCRFVKIKVGSEPEHDPHRVSVAKRAIGDQTLFVDANGAYTRK
                     QALALADTFAAEADIRWFEEPVSSDDLQGLRFIRERVPSPVEVAAGEYGYDLDYFRRM
                     LAAEAVDVQQADATRCGGITGFLQAASLCAAHHIDLSGHCAPAAHLHAACAAPRLRHL
                     EWFHDHVRIESMLFEGAPVAHGGVIRPDLSRPGLGLTFKHKDAARFASRVA"
BASE COUNT          515 a          972 c          973 g          485 t
ORIGIN      
        1 ggatagcgcg gacgcttcgg gccccaagga taagtcaccg gacccgcgat cttcagcgcc
       61 tgttcggctt cgccgtagta gtgccacatc tcccgccaat cgagcggcca gtcggcgccg
      121 taacccagca gggaccgtga cttgaaccat tcggggcgga atcgcagcga aaccatcgcg
      181 aaatgcaccg tactgccgcc taccgccttg ccgctgttct tgccccccat gatgatcggg
      241 ttctcgccgt cgatcaccct cgggtcggtc cagtaaagct tgttctgctc ggtctcgtcg
      301 gaagcgaagt cctccagcgg gcgaaaccat ggaccggcat cgaagccgat gacggagaag
      361 ccttgttcgg caaggcgggc gatcagcggc ccgccacccg cgccggtgcc gatgacaacg
      421 aagtctactt cttcggtttc cgggtattcg cgcatcggca cccagccgcc cacccgcatc
      481 acgtcaggcg cgcgtccgcc ggagccgcgc ggcgcggcaa agggatcagc cgacacgggc
      541 gttcttcctg gctgaagctg cctcctcgcc tggatgggcc tcggctgctt cccacgggtc
      601 gcgccggtcc aaagccatgc ggacatagcc gcgcggactg gccggtccgc cgaagccgat
      661 ctcgttccag gcggtgggat gggcgtagta ggcgtgggtt atgtcgggca ggacccgatg
      721 ggcgaagaag gttttccgcg gcatgtcgcc ccaggccgga tcgtgcagct cgtcccgctg
      781 catctggccg atcagcgtat cctgatcgga tgtcgggatc gcatgaaagc tctcgccatg
      841 tctggcccgc gcttccgcat cgagtgccgc caggccacgc cgccacgact cacgttcgtc
      901 cggcatgtcg gcgaatcgat agccgtcgcc ggcaccattc accatgcgct gatcgacata
      961 ggcggcgagc ggcaccgccg ggcgatttcc cggttgcggc aggatgcggg cgcacaacgc
     1021 cacgagcgtg gtccactcgg tcggcgtaaa gaattgcggc tggcgtggca ccgccaggcg
     1081 gcggtcgatc acctgccgcg tcacggcgtt ccatgacatc gtgtcacgtt tggccagcac
     1141 gtcgtagccg ggatagcggc cgttatcagt catcgggatg gtcctccaga agcccgagcg
     1201 cagccaaccc ggccaacgcc agtccggtga agctgggcgg tgcggggatc ggagggccgt
     1261 tcagcacgtt ctggcgccag ttccgccaac cgcccatgtt gcgggcgacg ccgaaggcgt
     1321 ggaatcccgc accggcaaac cccaatgccg ccgtcaagcg cagccaccac cgggtcagcg
     1381 gacggggtgt ccgcgccgcc ccgaccgcca cgccgccgag caacgccgcc gttacgggtg
     1441 ggatggtgac gggcgcatac atgaatgggt tgtggtaggc gccgcgaaag tgcaacagcc
     1501 cggcctccgc gaccgttccc aggatgccga tcccggtcgc cgccgctacg acgcgtccgg
     1561 cttgcaaacc cccaatcgtg ggaagcgtgg cgggcgcgtt atcgcgtacc cgttcggcca
     1621 ggtaacccgt ggcgccggac aggacgagcg ccgcgggggc gccgatgggt gccgaataga
     1681 aaaggttctg ccaggtaaag ccgcccggct tcctgctgat attgaagata tggaaagccg
     1741 tgccgaccag tcctgtcgca cctgccaacg catacacgcc gtcgcgcaaa cgatgcgcac
     1801 catgacggtg atcggcaacg ccatgaacgc ttatcccaag cgacagggcc gcggtgataa
     1861 gcggagcgaa catcgccgga ttatgaaaag atccgcggta gtgctccacc gcgctgtccg
     1921 ccagcaccga ggtcgccaga acccccgccg ctcggttgag gcgcctggcg gccctgaccg
     1981 ccggtgctgc ggaagaattg gtgtctgctt cgggataacc gcgttgggta cgctcccgga
     2041 tatcgtcatg gtgggcgaag gcccgccacc tacggcttgc ggtgcacgat ctatcgaggt
     2101 cgtcaacggt gggccttcgc gcaacgtgaa gttgccgcgg tagttcgctc ggttcgaagc
     2161 ggctttttgt catcaaaccg ttacgggcgg gcgagggaaa tgcggtgctg tggagttgct
     2221 cgccgcgtcg agccaccatc gccagcaggc ccaggcccac ggcgacgacc ggaaccgttg
     2281 ttgccgcggt tctgctttca accatgggct aggctacccg ggacgcgaat cgtgcggcgt
     2341 ccttgtgctt gaacgtcagg ccaagccccg ggcgcgacag gtcgggccgg attaccccgc
     2401 catgcgccac cggggcgcct tcgaacaaca tgctctcgat gcgaacgtgg tcgtggaacc
     2461 actccaggtg gcgcagccga ggggcagcgc aggcggcgtg aagatgggcc gccggggcac
     2521 agtgcccgga aagatcgata tgatgggctg cacagagcga cgccgcttgc aggaagccgg
     2581 tgatgccacc gcagcgcgtc gcatcggcct gctgcacatc gaccgcctcg gccgcaagca
     2641 tccggcggaa atagtcgagg tcgtagccgt attcgccggc ggcgacctcc actggcgacg
     2701 ggacgcgctc gcggataaag cgaaggcctt gcaggtcgtc ggatgagaca ggttcctcga
     2761 accatcgtat atcggcctcg gccgcgaacg tgtccgcgag cgcgagcgcc tgcttgcgcg
     2821 tataggcgcc gttggcatcg acaaacagtg tctgatcccc aatcgcgcgc ttggccacgg
     2881 acacgcggtg cggatcgtgt tcaggctcac tgccgacttt tattttaaca aaccggcagc
     2941 cgtca
//
LOCUS       DASWQN010000423         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1360192, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000423 DASWQN010000000
VERSION     DASWQN010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360192"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VGC82_06950"
     CDS             <1..727
                     /locus_tag="VGC82_06950"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0423046.1"
                     /translation="YIALSGALHAIGRAGQKPTPPLNLVGDYGGGALYLVTGLLAAII
                     SARPSGVGQVVDAAMVDGALSLMTPIYAMQAGGRMGNARGENQLDSGSPYYEVYECAD
                     GQFISIAPIEAKFHAELLRRLEIDPSSMPAQRDTMGWASWKALLTERFRSRTRDEWCD
                     LLEGTDACFAPVLSMREAYQHPHNQARAAFIDVGGHRQPAPAPRFGTTKLATPRPPGS
                     VPLADALAAWGLLEAELEMTLNA"
     gene            791..1543
                     /locus_tag="VGC82_06955"
     CDS             791..1543
                     /locus_tag="VGC82_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEY0423047.1"
                     /translation="MTGQIWIGFGALLRLGKWGNRMFLRSRTLAVAALLVATVVNTGA
                     LAPAARAQTSQQVTVDGARKVLNDLRHDKAFGNAAQLMRQAKAIFIVPKLIKGGFVVG
                     GEGGNGVLMVHNAGGWSDPGFYAIGAASFGLQIGLEQSEVIMLIMTQKGLDGVLRDQF
                     KIGAQAGITAVTLGSGVEGAIGGASLPDVVVWSSSTGVYGGLTVDGSIIRSQPNLDAA
                     FYGRPVTPRDVLFGKTQSPHAAGLRRDMSGIG"
     gene            1604..1677
                     /locus_tag="VGC82_06960"
     tRNA            1604..1677
                     /locus_tag="VGC82_06960"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1636..1638,aa:Gln,seq:ctg)
BASE COUNT          306 a          504 c          591 g          362 t
ORIGIN      
        1 ctacattgcc ttgtccggtg cccttcatgc catcgggcgg gccggccaga agccgacccc
       61 gccgttgaac ctggttggcg actacggcgg tggcgcactg tatcttgtca ccgggctgct
      121 cgccgcgatc atttccgccc ggccttcggg tgtcggtcag gttgtcgatg ccgccatggt
      181 cgatggcgcg ttgtcgttga tgaccccgat ctacgcgatg caggcaggtg gccggatggg
      241 aaacgcccgg ggtgaaaacc agctcgattc cggttcgcct tattacgagg tttacgaatg
      301 cgctgatgga caattcatct ccatcgcgcc gattgaggcg aagtttcatg cggagcttct
      361 tcgccggctg gagatcgatc catcgtcaat gcccgcgcag cgcgatacga tggggtgggc
      421 aagctggaaa gcattgctga ccgaacgctt tcgcagccgc acgcgcgatg agtggtgcga
      481 tctgctggag ggaaccgacg cgtgtttcgc gccggtgctg tcgatgaggg aagcgtatca
      541 gcatccgcac aatcaggcgc gcgctgcatt catcgacgtc ggcggtcatc gtcagccggc
      601 accggcgccc cgcttcggca cgaccaagct ggcaacgccg cggccaccag ggtcggtgcc
      661 gttggcggac gcactggcgg cctggggctt gttggaagcc gaattggaaa tgacgctcaa
      721 cgcctgagta catttgttcc gacttcaagt ctgcgccggt gaaaggaacc cgccgcgacc
      781 ggtttaattg gtgaccggac aaatctggat tgggttcggt gcgttgttgc gtcttggcaa
      841 gtggggaaac aggatgtttt tacgatcgag aactttggct gtcgccgcgc tgttggtagc
      901 gaccgtcgta aacaccgggg cgctcgcgcc ggcggctcgg gcacaaacaa gccaacaggt
      961 taccgtcgat ggcgcgcgta aggtgctcaa cgatctgcgt cacgacaaag cttttggcaa
     1021 cgcggcgcaa ctgatgcgcc aggccaaggc catcttcatc gtgcccaagc ttatcaaggg
     1081 cggcttcgtc gtcggcggcg aaggtggcaa cggtgtgctg atggtgcata acgccggcgg
     1141 ctggagcgat ccgggcttct atgcgatcgg cgctgcttcg ttcggcctgc agatcgggct
     1201 ggagcaatcg gaagtgatca tgttgatcat gacccaaaaa ggcctggacg gcgtgctgcg
     1261 ggaccaattc aagatcggcg cgcaagcagg gatcaccgcc gtaacgctgg gttccggggt
     1321 agaaggtgcg attggcggcg catccttgcc ggatgtggtg gtttggtcgt cgtccaccgg
     1381 ggtgtatggt ggtttgaccg tggatggatc gatcatccga tcgcaaccga acctggatgc
     1441 ggcgttctat ggccgaccgg tgacgcctcg tgacgtgctt ttcggcaaga cgcagagccc
     1501 tcacgcggcc ggcctgaggc gggacatgag cgggatcggc tgagtttcgc gcaacggatt
     1561 gcggcaggcg gatgggcgtg ctactccgcc ggccgcgcct gcatggggga tagtttagcg
     1621 gtaaaactcc cggctctgac ccgggcatcc ttggttcgaa tccaggtccc ccagccattc
     1681 catcactgtc tcctgtcatc gaggtccgac gtgatgggat catccgcgga attcgcggtg
     1741 tacggcggcc tttctccgcg tca
//
LOCUS       DASWQN010000424         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_436410, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000424 DASWQN010000000
VERSION     DASWQN010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436410"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VGC82_06965"
     CDS             <1..441
                     /locus_tag="VGC82_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY0423048.1"
                     /translation="AGLMDGSVSTLAPLFAAAFATHHNWQTFLVGLAASIGAGISMGF
                     AEALSDDGSLTGRGSPWLRGVTCGLMTTVGGLGHTLPYLVPDSWPNAFWIATAIAGVV
                     VFFELWAIAYIRARYMDTPFLQAVFQIVLGGAIVLAVGILIGAA"
     gene            524..1051
                     /locus_tag="VGC82_06970"
     CDS             524..1051
                     /locus_tag="VGC82_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY0423049.1"
                     /translation="MTDRLQYGTTAKVLHWLVAALLLVQYLIGWLMPDVHRGPPGKPM
                     TFHISFGILILTLIVVRFAWRLTHPVAPESSLPAWQRLSSEAVHWLLYLLVLATTLTG
                     WLFASFRGWSVSFFYLFPLPMLASDNPAGIKTIDGWHQAAEWTLLVVVGLHIVAALVH
                     IFIYRNRIMARMLPG"
     gene            complement(1176..>1571)
                     /locus_tag="VGC82_06975"
     CDS             complement(1176..>1571)
                     /locus_tag="VGC82_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0423050.1"
                     /translation="PRNGDVVQQVRDLTGGGVHHSFEVLGRKETAELAFAMLAPGGTA
                     TIVGMIPFGQKIELHGFDFLRERKIQGSSMGSNHFRVDMPRLVEFYMRGRLHLEDWIS
                     ARLKLSEINEGFAAMKAGKTVRSVIMFDS"
BASE COUNT          238 a          528 c          479 g          326 t
ORIGIN      
        1 gccggcctga tggacggctc ggtctcgacg ctggcgccgc tgttcgcggc cgcctttgcc
       61 acccatcaca attggcagac ttttttggtg ggactggcgg cctcgatcgg cgccggcatc
      121 agcatgggtt ttgcggaagc cttgtccgat gacggatcgc tcaccgggcg cggttcgccc
      181 tggctgcgcg gcgtcacctg cggcctgatg acgacggtcg gcggtctcgg ccacaccctg
      241 ccctacctcg ttccggactc ctggcccaat gcattctgga tcgcgaccgc cattgcgggc
      301 gtcgtggtgt tcttcgaact gtgggcgatc gcctatattc gcgcgcgcta catggacacg
      361 ccgttcctgc aggcggtatt ccagatcgtg ctcggcggcg ccatcgtgct agccgtcggc
      421 attctgatcg gggcggcata gcctgcgcgg agccgccagg catcccagtg ccacgccggc
      481 tttgccattc gaacagcccc tcgcaacctc ctccaggaat ccgatgaccg atcgtctgca
      541 atacggcacc acggccaagg tactccactg gctggtggcg gcgcttctgc tggtccaata
      601 cctgatcggc tggctgatgc cggacgtcca ccgcggccct cccggcaagc cgatgacgtt
      661 ccacatttca tttggaattc tgattctgac cctcatcgtc gtgagattcg cctggcggct
      721 gacgcatccg gtggcgcccg aaagctcgct tccggcctgg cagcggctga gttcggaggc
      781 cgtgcactgg ctgctgtatc tgctggtgct ggcgaccacg ctgaccgggt ggctgttcgc
      841 ctcgtttcgc ggctggtcgg tgtctttctt ctatctcttt ccgctgccga tgctggcctc
      901 cgacaacccg gcgggaatca agaccatcga tggctggcac caggcggcgg aatggacgct
      961 cctggtcgtg gtcgggttgc acatcgtcgc cgcgctggta cacatcttca tctatcgcaa
     1021 ccgcattatg gcgcggatgc tgccgggata ggcttttcag ttcaagacca gtcattccgg
     1081 agcgcgcgtg aaacgagcga acccggaatc cggaagttgt tggcaggcgt tttccgggtt
     1141 cccggtcgcg cgccctgcaa tgacgcggcc cgcttctagc tgtcaaacat gatcacgctg
     1201 cgcaccgtct tgcccgcctt catcgcggca aagccctcgt tgatctcgga cagcttcagc
     1261 ctggccgaga tccagtcctc cagatgcagg cgcccgcgca tgtagaactc gaccagccgc
     1321 ggcatgtcga cccggaaatg gttcgagccc atcgacgagc cctggatctt gcgctcgcgg
     1381 agaaaatcga aaccgtgcag ttcgatcttc tggccgaacg ggatcatccc gacgatggtc
     1441 gcggtgcctc cgggcgctag catcgcgaac gccagttccg cggtctcctt gcggcccagc
     1501 acttcgaacg aatggtgcac cccgcccccc gtgagatcgc gcacctgctg caccacgtcg
     1561 ccattgcggg g
//
LOCUS       DASWQN010000425         6274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1756214, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000425 DASWQN010000000
VERSION     DASWQN010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756214"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VGC82_06980"
     CDS             complement(<1..235)
                     /locus_tag="VGC82_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY0423051.1"
                     /translation="MMAATTEAQHDHGHGDDHAHDHKPTFITRWFFSTNHKDIGTLYL
                     AFSLFTVLFGLVFSIIMRAELQAPGQQFIQDGQV"
     gene            complement(303..1136)
                     /gene="coxB"
                     /locus_tag="VGC82_06985"
     CDS             complement(303..1136)
                     /gene="coxB"
                     /locus_tag="VGC82_06985"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY0423052.1"
                     /translation="MRIVKLLLVAMAGLAFSLQAAPLAWAQGVVGAPQAWEMGMQKAF
                     GPIKERQIELHNLVLVIITLITVLVGALLIWVMIKFNARRNPVPSQTSHNTILEVAWT
                     VVPVLVLVVIAIPSFRQIYYQDRTPDPDITIKVTGHQWYWEYAYPDQGNITIESRYIH
                     DEDLKPGQLRLLDVDSQLIIPVGKKVRVLTNSSDVIHSFFIPAFGIQRYAIPGRTIET
                     WMEADQVGTFYGECNQICGQNHNRMPISVRAVSEEDFKAWAATAKKSAAAGQLPKIAS
                     R"
     gene            complement(1303..1977)
                     /locus_tag="VGC82_06990"
     CDS             complement(1303..1977)
                     /locus_tag="VGC82_06990"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0423053.1"
                     /translation="MNHTLRKQSAGVGRMVPAAALDQASIVEAYRRWAGIYDKAFGGI
                     SSKGRLRAVSLINRLSGRDVLEVGVGTGLALPHYHAEKRITGIDLSKEMLRKARERVR
                     TDGLKNVQALHEMDAEATSFADASFDLAVGMFVASVVPNPRRLLAEMRRLVRPGGDIL
                     LINHFAAAKGPRWWVERAMAPASRALGWHPDFAMDAIFSAEDLARIEAESIPPLGIFT
                     LVRLRV"
     gene            complement(2155..2397)
                     /locus_tag="VGC82_06995"
     CDS             complement(2155..2397)
                     /locus_tag="VGC82_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY0423054.1"
                     /translation="MGSFSIWHWLVVLAVILVLFGGGSKISGLMGDFAKGIKSFKKNM
                     AEPDDASMEESAVKPSGSLAGPGAQAAPTRESTTTR"
     gene            complement(2532..2993)
                     /locus_tag="VGC82_07000"
     CDS             complement(2532..2993)
                     /locus_tag="VGC82_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY0423055.1"
                     /translation="MHLIAIGRLRAGPELELFDRYDSRLRPRLRVVEVPEERGNASLI
                     KRKEGAALLAALPKDAFTVALDLGGHALDSEGLALRIDQWRGLSHPICFLIGGAEGLD
                     APVIAKANYVLSLGLMTWPHFLVRAMLAEQLYRAQTIGQGHPYHRAGRPEV"
     gene            3091..3165
                     /locus_tag="VGC82_07005"
     tRNA            3091..3165
                     /locus_tag="VGC82_07005"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3123..3125,aa:Val,seq:gac)
     gene            complement(3201..6263)
                     /locus_tag="VGC82_07010"
     CDS             complement(3201..6263)
                     /locus_tag="VGC82_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0423056.1"
                     /translation="MVLVGLAAFPFLPVAPLPRVDFPTIAVNAKFPGASPETMASTVA
                     QPLERQFAQIPGMAQLTSISVLGQSQITLQFDLDRNIDAAAGDVQAAINGASGQLPKA
                     LPSSPTYYKVNPADSPILILSVQSDELPMINVDDYADVVLSQQISQIAGVSQVFIGGE
                     QKRAVRVQVDPAKLAAMGMTLEDVRGVLVNATVNSPKGTIESGPQSFAVYNNDQLTKA
                     AEYDNVILAWRNGAPVRVRDIGRAVDGPENQLLGGWQNGKRGILLVIFKQPGANVIDT
                     VDHIKAALPQLEASIPPSIHVGVVMDRTLTIRASVADVQFTLILSIALVVMVIFLFLR
                     SVWATIIPSITVPVALISTLAVMYLLGYSLDNLSLMALTIAVGFVVDDAIVMLENIFR
                     HIEDGMSPMEAALKGAGEIGFTIVSISLSLIAVFIPLLLMGGIVGRLFREFAMTVTIA
                     VVVSAFISLTLTPMMCSRFLKHHTGGHNWAYRIVEGFFTTLLNFYRRTLDIALHFRFI
                     TLLVFVATLSTTVYLYIVIPKGFFPAQDTGVVLGTTKGAQDISFHEMGEVQQKLNEIV
                     AADPDTAAYGATVGAGVSGQTQNSGRMFIALKPWDQRVGGTAQQFIARLRPKFASVSG
                     GTATLQAAQDIKVGGRMTDAEFQYTLQDANLEELFEWSPKVLAKLRSLPLLRDVSTDN
                     QVGGATVTMTIDRDKAARFGVQPQVIDDTLYDAFGQRQITQYFTQVNSYHLVLEVMPG
                     DAADVETLGKLYVHSNTGQSVPLSSFVKWSTAPVQPLSISHQSQFPATTISFNLAAGV
                     ALGEAVDAISTAMRDMGTPVTLQASFQGTAQAFRSSLASQPYLIAAALITVYIILGIL
                     YESYILPLTILSTLPSAGVGAILMLMVVHYDLTVIALIGIILLIGIVKKNGIMMVDFA
                     INAERRDGQTPLAAIRQACLLRFRPIFMTTMAAMLSGLPLMLGTGAGSELRRPLGFAM
                     VGGLALSQILTLYTTPVIYLYLDQLQHWLAPRRSRMPALAAKMGAEAD"
BASE COUNT         1317 a         1936 c         1821 g         1200 t
ORIGIN      
        1 agacctgtcc atcctggatg aactgctgcc ccggagcctg gagctcggca cgcatgatga
       61 tcgagaacac caatccgaac aggaccgtga acagggagaa cgccaggtaa agggtgccga
      121 tatccttgtg attggtgctg aagaaccaac gcgtgatgaa agtcggctta tggtcatgcg
      181 cgtggtcgtc cccgtggcca tggtcgtgtt gtgcttcagt cgtcgcagcc atcatctttc
      241 tccagtgggc tgccggccct caggccgaca caggatgccg ccgatatcgt ccatagtcat
      301 gatcatctgg aggctatttt tggaagttga cccgccgcgg cggatttttt tgcggtcgct
      361 gcccaagcct tgaagtcttc ctcggacacc gcccgcaccg agatcggcat tcggttgtgg
      421 ttttggccgc agatctggtt gcactcgcca tagaacgtgc cgacctgatc cgcctccatc
      481 catgtctcga tggtgcggcc gggaatcgca taacgctgaa tgccgaatgc cgggatgaag
      541 aaactatgga tcacatccga actgttggta aggacccgta ctttcttacc cacgggaatg
      601 atcagctggc tgtccacatc gagcaggcga agctgtcccg gcttcaggtc ctcgtcgtga
      661 atgtagcgac tttcgatggt tatattgcct tggtccggat aggcatattc ccagtaccac
      721 tgatggccgg tcaccttgat ggtgatatcg ggatccggcg tccgatcctg ataatagatt
      781 tgccggaagg acggaatggc gatcaccacc agaaccagca ctggaacgac cgtccacgcg
      841 acctctagaa tcgtattgtg gctggtctgg ctcggaaccg gatttcgccg cgcgttgaac
      901 ttgatcatca cccagatcaa cagggcgccg accaggaccg taatcagcgt aatgatcaca
      961 agcacaaggt tatgaagctc gatctggcgc tccttgatcg gcccaaaagc cttttgcatg
     1021 cccatttccc aggcctgcgg cgccccgacc accccctgcg cccaagccaa cggggcggcc
     1081 tgcaggctga acgccaaccc agccatggcc accaggagca acttaacaat ccgcatcgac
     1141 tgattacccc aacctatcag agaccaagcg ccgcgggtcg atacacgacg cgcgctcctt
     1201 ccgttcgcct agcggacgca ggatacaaga tcaaggtgtc aggagaagct tgcaacgctt
     1261 tcggccgaat ccgtgccgga ttcacagccg gccattactg gatcatacgc gcagacgaac
     1321 caacgtgaaa attcccagag gaggaatcga ctccgcctcg atccgggcaa gatcttcggc
     1381 ggaaaaaatt gcatccatgg caaaatcagg gtgccagcca agcgcgcgag acgccggtgc
     1441 cattgcacgc tccacccacc atctcggtcc cttggcagca gcaaaatgat tgatcagcag
     1501 aatgtctccg cccggtctta ccagccggcg catctcagcc agcaggcggc ggggattggg
     1561 gacaaccgag gcgacgaaca ttccaaccgc gagatcgaac gaggcgtccg caaacgatgt
     1621 cgcctcggcg tccatttcat gcagcgcctg tacgttcttc aaaccatcgg tgcggacccg
     1681 ttcccgagcc ttgcgcagca tttcctttga aaggtcgatg ccggtgatgc gcttttcggc
     1741 gtgatagtgt ggcaaggcaa gaccggtgcc tacgccaacc tccagcacat ccctgccaga
     1801 caggcggttg atcaaggata ctgcccgcag cctacctttc gatgagatgc caccgaaggc
     1861 cttgtcataa atgcctgccc atcgccggta tgcctcgacg atggaagctt gatcaagtgc
     1921 cgcagccggg accattcgtc cgaccccggc cgactgctta cgaagagtat gattcatccg
     1981 ccttaaagcg cccttctgcc aatcgccaaa gctttggaaa tcgtcaaagc atccggccat
     2041 gccgcgcgac aatcatccgc acgataagga cgccttagat tacgcaaggt tgcggcgcaa
     2101 gccggcccgg aggccaaact cgcggcgttg tgcccgatga gccgtgacca gcgatcagcg
     2161 cgtcgtcgtt gattcgcgcg ttggcgcggc ctgggcgccg ggaccggcga gcgaaccgct
     2221 gggcttgacg gcgctttcct ccattgaagc gtcatccggc tccgccatat ttttcttgaa
     2281 cgacttgatg cctttggcaa agtcacccat taaaccgctg atcttgcttc caccgccgaa
     2341 cagcacaagg atgacggcca gcacaaccaa ccagtgccag atgctaaagc tacccatatg
     2401 tcctccaaac cgctgcgatg aaactgatgc cgctcgggcg gtcgttgttc aagctcgcgt
     2461 gaaacaaccg tccgggtgag tgacatggcg cgagtcggcg cgtgatgcaa gcatcttgtc
     2521 ttggtgcgag gttacacctc tggccggcca gcgcgatgat aaggatgacc ctggccaatc
     2581 gtctgggcgc gatacaattg ttccgccagc attgcgcgaa ccagaaaatg cggccaggtc
     2641 attaaaccca gcgaaagcac gtagttggct ttggcgatga caggcgcatc gagaccttca
     2701 gcacctccga tcaggaaaca tatcgggtga gacaggccgc gccactgatc gatccgaagc
     2761 gccagtcctt cgctatccag tgcgtgcccg cccagatcca gcgccaccgt gaacgcgtcc
     2821 ttcggcagcg ccgcgagcaa cgcagcacct tccttgcgct ttatcagcga cgcgttgccg
     2881 cgctcttccg gcacttcgac aaccctcagc cgcggcctga gccgtgagtc ataacggtcg
     2941 aacaactcaa gttcaggacc ggcacgcagt cgtccgatcg cgatgaggtg catggataga
     3001 gaacttttct tgctggacga tccggtcttg cggtgtgcca gtcttgcggg atggttgcgg
     3061 cacgcgctaa acacgggacc ccggcaaagc gggcgcgtag ctcagcggga gagcacctcc
     3121 ctgacacgga gggggtcaca ggttcaatcc ctgtcgcgcc caccattccc ttcggcacgt
     3181 aatcccgcgg gtcgtgcgga tcagtccgcc tccgccccca tcttcgccgc caaggccggc
     3241 atccgggatc ggcgcggtgc cagccaatgc tggagttggt ccaggtaaag atagatcacg
     3301 ggtgtggtgt aaagcgtcag gatctgactg agcgccaacc ccccgaccat ggcgaatccc
     3361 agcggcctgc ggagttccga cccggcgccg gtgcccagca tcagcggtag acccgacagc
     3421 atcgccgcca tcgttgtcat gaagatcgga cggaatcgca acaggcacgc ttgccggatc
     3481 gcggccagcg gcgtctgccc atcccttcgt tccgcgttga tggcgaaatc gaccatcatg
     3541 atgccgttct ttttcacgat ccctatcaac aggatgatgc cgatcaacgc gatgaccgtc
     3601 aggtcgtaat gaaccaccat gagcatcagg atcgcgccca ccccagccga gggcagcgtg
     3661 gacagaatgg tcagcggcag gatgtagctt tcgtacagga tgcccaggat aatatagacc
     3721 gtgatcaggg ctgcggcgat caggtaaggc tggctggcca gcgatgacct gaatgcctgg
     3781 gcggtccctt ggaaggacgc ctgcaacgtt accggtgttc ccatatcgcg catcgccgtc
     3841 gagatcgcat cgacggcttc gccgagtgcc acgcccgcgg ccaggttgaa tgatatcgtc
     3901 gtggccggga actggctttg gtgactgatc gaaagcggtt gcacaggggc ggtggaccat
     3961 ttcacgaacg aagacagcgg tacgctctgg ccggtattgg agtggacata aagcttgccc
     4021 agggtttcta cgtccgcagc atcgccgggc atgacctcaa ggaccaggtg ataagaattc
     4081 acctgggtaa aatactgagt aatttgtcgc tgaccgaacg catcgtacag cgtgtcgtcg
     4141 ataacctgcg gctgcacccc aaagcgggcg gccttgtcgc ggtcgatcgt catcgtcacc
     4201 gtcgcgccac cgacctggtt gtccgtactc acgtcgcgca gcagcggcaa tgaccgcagc
     4261 ttcgccaaca ccttgggcga ccattcgaag agttcctcca gattggcatc ctgcagcgtg
     4321 tactggaact cggcgtcggt catccgcccg ccgaccttga tgtcctgcgc ggcttgcagc
     4381 gttgccgtgc ccccggacac ggaggcgaac ttcggacgca ggcgcgctat gaactgctgc
     4441 gccgtcccgc cgactcgctg atcccatggt ttcagggcga taaacatccg accggaattc
     4501 tgcgtctggc ccgacacgcc cgcgcccacg gtcgcgccat aagccgcggt gtccgggtcc
     4561 gccgcgacga tttcgttcag cttttgctga acctcgccca tttcgtggaa ggatatatcc
     4621 tgcgcgcctt tcgtcgtacc cagaacgacg ccggtgtcct gcgccgggaa aaagcccttt
     4681 gggatgacga tataaagata aaccgtcgtc gagagcgtcg ccacgaacac caacaaggtg
     4741 atgaagcgga aatgcagcgc gatatcgagc gtccggcgat agaagttgag caaagtcgtg
     4801 aagaaccctt cgacgatccg gtatgcccag ttatggcccc cggtgtggtg cttcaggaac
     4861 cgcgaacaca tcatgggcgt cagggtcagc gagatgaagg ccgagaccac aaccgcgatg
     4921 gtgacggtca tcgcgaactc ccggaacaag cgtccgacga taccgcccat cagcaacagc
     4981 gggatgaaca ccgcgatgag cgacaagctg atggagacga tggtgaaccc gatctcaccg
     5041 gcccctttta atgccgcctc catcggcgac atgccgtctt caatgtgccg gaagatattt
     5101 tccagcatga caatcgcgtc gtccacaacg aagccgaccg ctatcgtcag tgccataagc
     5161 gacagattat ccaggctgta tcccagaagg tacatgaccg cgagcgtgct gatcagcgcg
     5221 accggcacgg taatcgacgg gattatggtg gcccaaacgc tgcgcaggaa caggaagatg
     5281 accatgacca ccagggcgat ggacaggatc agcgtaaact gaacgtcggc gacggaggct
     5341 cggatcgtca gcgtccgatc cataaccacg ccaacatgga tcgacggtgg gatcgaagct
     5401 tccagttgcg gcagcgccgc tttgatgtga tcgacggtgt cgatcacatt ggcccccggt
     5461 tgcttgaaga taaccaagag gattccgcgc ttgccgttct gccagccgcc aaggagctga
     5521 ttttccggcc catccaccgc tcggccgata tcgcgcaccc tgaccggcgc gccgttgcgc
     5581 cacgccagga tgacgttgtc gtactccgcc gctttcgtta gctgatcgtt gttatacact
     5641 gcgaacgact gcggtcccga ctcgatcgtg cccttcggcg aattcaccgt cgcgttcacc
     5701 aggaccccgc gaacgtcctc cagcgtcatg cccatcgcgg ccagtttggc gggatcgacc
     5761 tggacgcgca ccgcccgctt ctgctcaccg ccgataaaga cctgggatac gccggcaatc
     5821 tgagagattt gctgcgacag cacgacgtcg gcgtaatcgt ccacgttgat catcggcagt
     5881 tcgtcggact ggaccgacag gatcaggatc ggcgagtcgg ctggattgac cttataatag
     5941 gtcgggctgg acggcagcgc tttcggcagc tgaccactgg cgccgttgat cgcggcttgt
     6001 acatcgcccg cggccgcatc gatgttgcga tctagatcga actgcagcgt gatttgcgac
     6061 tgccccagga cgctgatcga cgtgagctgc gccattccag ggatctgcgc gaactgcctt
     6121 tccaacggct gtgctacggt actcgccatg gtttcggggc tggcgccggg gaatttcgcg
     6181 ttgaccgcga tggttggaaa gtccacgcgc ggcaggggtg ccaccggcag gaacggaaag
     6241 gcagcgagcc ctaccaggac cagggcagcc gtca
//
LOCUS       DASWQN010000426         1753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2151930, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000426 DASWQN010000000
VERSION     DASWQN010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151930"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VGC82_07015"
     CDS             complement(<1..851)
                     /locus_tag="VGC82_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0423057.1"
                     /translation="MAKRIAVIGAGAVGGYAGAHMAAAGHDVTLIDPWPDHVEQMKAH
                     GMEIFGMTPEERMTVKVNAMHLTEVQNLAKQRPIDIAFVSVKSYDTIWATTMIRQYLS
                     ASGCVVSMQNCINEERVASVVGWGRTLGCIVTGGVGVDLYQPGHIRRGYAKRADITSF
                     YVGEPSGKITRRSQEIAEIMSSIDASKATDNLWGERWTKLCVNGMRNGVSAATGMNGN
                     ARDHHAAVRRVCIQLAGEAVRVGQKLGYKLGKIGALPPEMVAEAMEGDAKSYAEIERL
                     MTQGGAG"
     gene            complement(910..>1753)
                     /locus_tag="VGC82_07020"
     CDS             complement(910..>1753)
                     /locus_tag="VGC82_07020"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0423058.1"
                     /translation="GRGCAMKMLSEAAVRQYREQGYYAPIRVMPREQAAVLRGKLEAF
                     EAGSGTLAGKLRHKSHLLFTWLNELVRHPTILDAVEDVIGPNILCWGSSFFIKEPRNP
                     GFVSWHQDSTYWGLDPADVVTAWVALSDSTAANGAMRVIPGSHRLDQVPHRDTFRHDN
                     LLSRGQEVMVDVDERRAAMLALSAGEMSLHHVRLIHGSDPNPSDLRRIGFAIRYIPTT
                     VRQIAGSHDHATLVRGVDTYGHFEPEESPDSDMSARAVAYHEAVTGAHAAILMRETGK
                     PMRA"
BASE COUNT          309 a          609 c          506 g          329 t
ORIGIN      
        1 tggccggcgc cgccctgcgt catcagccgc tcgatttcag cataggactt tgcgtcgcct
       61 tccatcgcct cggcgaccat ttcgggtggc agcgcaccga tcttgccgag tttgtagccc
      121 agtttctgcc cgacccgaac cgcctcccca gccaactgga tgcagacccg gcgaaccgcc
      181 gcgtggtggt cgcgcgcatt cccgttcatg cccgtggcgg cagagacgcc gttgcgcatg
      241 ccgttcacgc acagtttcgt ccaacgttca ccccaaagat tatcggtcgc cttcgacgca
      301 tcgatgctcg acatgatctc cgcgatctcc tgcgatcgcc gggttatctt gccgctgggt
      361 tcgccgacat agaacgacgt aatatcggcg cgcttggcat agccgcggcg gatatggccg
      421 ggctgataca gatcgacgcc gacgccgccg gttacgatgc atcccagtgt ccggccccag
      481 ccgaccacgc tcgcgacacg ttcctcgttg atgcaattct gcattgaaac cacgcagccc
      541 gaggccgaca gatactgccg gatcatcgtc gtcgcccaga tcgtgtcgta tgacttgacc
      601 gacacgaacg cgatgtcgat cggccgctgc ttggccaggt tctgcacctc ggtcaggtgc
      661 atggcattga ccttcacggt catccgctcc tcgggggtca tcccgaagat ctccatccca
      721 tgcgccttca tctgctcgac atggtccggc cacggatcga tcagcgtgac gtcgtgaccc
      781 gcggcagcca tatgagcacc cgcatagccg ccgacagctc cggcgccgat taccgctata
      841 cgcttcgcca tcgtctcgtt tcccctctga tttcgaaccg gcttacagcg gaatgccggc
      901 ggccgacagt caggcgcgca tcggcttgcc tgtttcccgc atcaggatcg ccgcatgggc
      961 gcccgtcacg gcctcatgat acgccaccgc ccgcgccgac atatcggagt cgggcgattc
     1021 ctccggctcg aaatgaccgt aggtatccac accgcgcacc aacgtcgcat gatcgtgcga
     1081 gccggcgatc tggcgcaccg tcgtcgggat gtagcggatg gcgaagccaa tgcggcgcag
     1141 gtcggacgga tttgggtccg acccatggat cagccgcaca tgatgcagcg acatctcccc
     1201 ggccgaaagc gccagcatcg ccgcccgtcg ttcatcgaca tcgaccatga cttcctgccc
     1261 gcgactgagc aggttgtcgt gccggaaggt atcccggtgc ggcacctgat ccagacgatg
     1321 cgagcccggt atcacccgca tcgccccatt cgccgcggtg ctgtccgaca gcgccaccca
     1381 agcggtcacg acatcggcgg ggtccaggcc ccagtaggtt gaatcctggt gccacgagac
     1441 gaagccaggg ttgcgtggct ccttgatgaa gaacgacgaa ccccagcaca ggatattggg
     1501 cccgatgaca tcctcgaccg catcgagaat ggtgggatgg cgtaccagtt cgttcagcca
     1561 ggtgaacagc agatgggatt tgtgccgcag tttgccggcc aacgtgccgg agcccgcctc
     1621 gaacgcctcc agcttgccgc gaaggaccgc agcctgttcc ctcggcatga cgcggatcgg
     1681 ggcgtaatat ccctgttccc ggtattggcg aaccgccgcc tcgctcaaca tcttcatcgc
     1741 gcacccccgg ccg
//
LOCUS       DASWQN010000427         4639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_172828, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000427 DASWQN010000000
VERSION     DASWQN010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172828"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VGC82_07025"
     CDS             complement(<1..293)
                     /locus_tag="VGC82_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0423059.1"
                     /translation="MSTIDGATLLAESLKQQGVQYMFGVVGFPVGPLAEAAQKAGIPY
                     IGMRNEQTASYAAGAVGYLTGRPGSCIVVTGPGVIHGLAGLANAQQNCWPMLL"
     gene            484..1275
                     /locus_tag="VGC82_07030"
     CDS             484..1275
                     /locus_tag="VGC82_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0423060.1"
                     /translation="MSQRRRDTVAYDNVSIDVILDEPDTQPADPLVLLPSSSRDSEDF
                     DEISAMFAAAGFRVLRPQPRGMCGSIGPMEGLTLHDYAKDVAMVIERFAGGPAFVLGH
                     AFGQWVGRCVAADHQHLVRGMILAAAAAKAADPALREHLARCVDTSLPDPVRLAALQV
                     VFFAPGHDPSAWLGNWHPVASKSQRAASAATPQGDWWGAGSAPVLDIQAELDPWRPPE
                     TRTGISDDLGAGRVTVVVIPDASHALIPEQPEAVVRAVRNWARTV"
     gene            1297..2415
                     /locus_tag="VGC82_07035"
     CDS             1297..2415
                     /locus_tag="VGC82_07035"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY0423061.1"
                     /translation="MTTPRSEPPFRADHVGSLLRPKVLQEARAKWKSGSLPLDALREV
                     EDTCIAAAIAKQEDIGLRAATDGEYRRAYWHYDFVSGLDGVEIYEPEEKIQFQGNVPL
                     MHKLRVNSRIGWTKPTMIDHYQFLASHVRTAVAKQTIPSPSVVHFRGGREAIDRATYP
                     TMEPFFADLGAAYNKAVKAFGEAGCRYLQLDEVNIAYLCDPEQIAMLKARGEHVEDIL
                     GIYARMLNQAIAGRPAGMTISMHLCRGNFRSTWIASGGYEPVAEVLFNQIDSDAYFME
                     YDTDRAGGFEPLRFVPRGRKMVVLGIMTSKTGELESKDVLKRRIDEAAKFLPLEQLAL
                     SPQCGFASTEEGNTLTEDQQWAKLSRCVEVAREVWGGV"
     gene            2768..>4639
                     /locus_tag="VGC82_07040"
     CDS             2768..>4639
                     /locus_tag="VGC82_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423062.1"
                     /translation="MTHTYDRDQTMPRRLAIVLTTLFLTSLVCLSLMTFDHVKYIALQ
                     ARGHLSAETIRGALTVSDWGILCVTTALALALIALPAARKGFVTLWSKDTLLVWMPVG
                     AALFWCGHAILGSGLIVTGDAGTHVARVNHLAMALRDGGSLFWDNYFFGGSTLLQFTG
                     PVFHWTAALIDLAVEDPTQSIKFAAFCIRLIAALFMYLLMRGYGLSRTTGSLTALFYG
                     GAFFMTYMEIVRSSFPQLINFAAMPAIIFFVERILAGRAVIGGGTAGLSLVAILFIGN
                     HQPTALIFGLLVAIYVCIRLSMTGGNGATMVALAATAMFIAVGSVFFLLPFVLERGMT
                     ADDFSAASLITFAWPSVATLHDFVVWGSAGAGPVYAAYVGLPMLGCVAAGGYAIWTNS
                     QTLERSLRSTWSLMLGLASISLFVRGAYVREATFTFFFLCVAAGVGLEMATRGHRRGS
                     LLAAVFVTTLLDAGPLAVQPWTRRDFVPIAEAGEYLVDRARNARVVEVQQIDGKAYIA
                     ADPTLSPLAYGRLQILAGPHKQDATKAHNGFAALLKIAQQDLRTDGRLEPRTRNMLAV
                     ANVGWVVGTAAKGMGLPNNLEGTITDPVLGTYLPIPEATPFIVSGRLETMTRPMAFDV
                     "
BASE COUNT          843 a         1367 c         1463 g          966 t
ORIGIN      
        1 atcaatagca tcggccagca attctgctgc gcgttcgcaa ggccggccag gccatggatg
       61 acgccggggc cggtcacgac gatgcacgat cccggacgac cggtcagata gccgacggca
      121 ccggcggcat aggacgcggt ttgctcatta cgcatcccga tataggggat accggccttt
      181 tgggcggcct cggcgagcgg acccaccgga aagccgacca ccccaaacat gtactggacg
      241 ccctgctgct ttaggctttc cgccaacaag gtggcgccgt cgatcgtgct catgcgatgt
      301 tctccccttc tgtcgcggcc ggaaggtagc tggcgtccgg gtgcagtcgt attcccggtg
      361 cctgtgaccg cgttatcacg agagaatgac tgcttatcac cgcgctgaaa agggctggcc
      421 ccaccctggt tccataccgc cctccctcag gctagattac ggccagaaca gggagggtgg
      481 cgaatgagcc aacgccggcg ggataccgtc gcttacgaca atgtctcgat cgatgtgatc
      541 ctggacgagc ccgacacaca gccggctgat ccgctggtgc tgttgccctc ctcgtcgcgc
      601 gattccgagg attttgacga gatttccgcg atgttcgccg cggcgggctt tcgggtgctg
      661 cgcccgcagc cgaggggcat gtgcggcagc atcggcccga tggagggact aacgctgcat
      721 gactacgcga aagacgtggc aatggtgatc gagcgtttcg ccggcggccc cgccttcgtt
      781 ctgggccatg ctttcggcca gtgggttggg cgatgcgtgg cggcggatca tcagcatctg
      841 gtgcgcggca tgatcctggc agcggctgcg gcgaaggcgg ccgatccggc gctacgcgaa
      901 cacctggcga gatgcgtcga taccagtctg cccgatccgg tccggctcgc cgcactccag
      961 gtcgtcttct ttgcccccgg ccatgatccg tccgcttggc tgggaaactg gcatccagtg
     1021 gcatccaaga gccagcgcgc cgcaagtgcc gcgactccgc aaggcgactg gtggggtgcc
     1081 gggtccgccc ccgtgctgga cattcaggcc gaactggacc cctggcggcc gcccgagacc
     1141 aggactggca tcagcgatga tcttggcgcc ggccgtgtca ccgtcgtggt catcccggac
     1201 gccagccatg cgttgatccc tgaacaaccc gaagccgtgg tccgagcggt gcgcaattgg
     1261 gcccggacgg tctgactttc gccaggagaa cccatcatga cgaccccacg ctccgaaccg
     1321 cctttcagag ccgatcatgt cggcagcctg ctccgtccga aggtgctgca agaggctcgg
     1381 gctaaatgga aatccggctc gctgccgctg gatgccctgc gtgaggtgga agacacatgc
     1441 atcgccgccg ccatcgccaa gcaggaagac atcggcctgc gcgcggcgac cgatggcgag
     1501 taccgccgtg cctactggca ctatgacttc gtttctggcc tggatggtgt cgagatctac
     1561 gagccggaag agaaaatcca gttccagggc aatgtcccgc taatgcacaa gcttcgcgtc
     1621 aacagccgga tcggctggac gaagccgacg atgatcgacc attaccagtt cctcgcgagc
     1681 catgtgcgga cggcggtggc gaagcagacc atcccgtcgc cgtcggtggt gcatttccgc
     1741 ggcgggcggg aagcgatcga tcgggccact tatccaacta tggaaccatt ctttgccgat
     1801 cttggtgctg cctacaacaa agccgtcaag gcgttcgggg aagccggctg ccggtatcta
     1861 caactggacg aggtcaatat cgcctacctg tgcgatcccg aacagatcgc gatgctgaag
     1921 gcccgcggcg agcatgtcga ggatattctg ggtatctacg cccgaatgct gaaccaggcg
     1981 atcgcgggac ggccggcggg aatgacgatc tccatgcatc tgtgccgcgg caatttccgc
     2041 tccacatgga ttgcatcggg cggctatgag ccggtggccg aggtgctgtt caaccagatc
     2101 gattcggatg cgtatttcat ggaatatgac accgatcgcg ccggcgggtt cgaaccgctg
     2161 cgcttcgtgc cacgcggaag gaagatggtc gtgctgggga tcatgaccag caagaccggc
     2221 gagcttgaaa gcaaggacgt tttgaagcgg cgcatcgacg aagcggcgaa gttcctgccg
     2281 ctggagcagc tagcactgtc gccgcaatgc gggttcgcct cgaccgagga aggcaacacg
     2341 ctgaccgagg accagcagtg ggcgaaactg tcccgctgcg tggaagtggc gcgggaggtt
     2401 tggggcgggg tttgaccgcg gatggacggg gtggacggcg accggcatag caggctaagg
     2461 taggctagaa tcttacaacg acctgtcacc aggctggcct aaaccgctgg ggatctcgcc
     2521 gaggcacgag ctgcacaatt gccggtaaat cgccgctatc gcacacctta tagtgccacc
     2581 ccgaggtcat tgcctgcggc gaatgccgct cacgcgattt tcgccgtttg aatcatttgt
     2641 ttgaattgtt tgtttgaacg ccggcatctg agtataatgg ttccgtaacg gaaccgatgg
     2701 cccaacgcca gtcccgctaa cgaaagcggt tcctcgaacc gattgtttag cgtgcgaagg
     2761 cccagcgatg acccatactt acgatcgcga tcagacaatg ccaaggcgac tggccatcgt
     2821 cctgaccacg cttttcctga cgtcgctcgt gtgcctgtcc ttgatgacct tcgaccatgt
     2881 gaaatatatt gcgctgcagg cccgaggcca tctttccgcc gagacgatac gcggcgccct
     2941 gaccgtttcc gattggggta tcctttgtgt gacaaccgcg cttgcgctgg cgttgattgc
     3001 gctccccgcg gcgcggaaag gttttgtcac actgtggagc aaagacaccc tgcttgtgtg
     3061 gatgcctgtt ggggcggcac tattctggtg cggccatgcc attctgggca gcggcttaat
     3121 cgtgaccggc gatgcaggga ctcacgtcgc tagagtcaat catcttgcca tggctctgcg
     3181 cgacggcggc tcattgttct gggacaatta cttcttcggt ggctcaactt tgttgcagtt
     3241 caccgggcct gtgtttcatt ggaccgcggc gctgatagac ctggcggtgg aagatccgac
     3301 gcagagcatc aagtttgcgg ccttctgcat tcggctgata gccgcattat tcatgtatct
     3361 tctgatgcgc ggttatggct tgagtcgtac gactggaagc ctgacggccc tgttctacgg
     3421 cggcgcgttc ttcatgacat atatggagat cgttcgttcc tcgtttccgc aactgatcaa
     3481 tttcgccgcg atgccggcta tcatcttctt cgtagaacga atactggccg gccgtgccgt
     3541 tatcggtggc ggcacggctg gtttatccct tgttgcgatc ctgttcatcg gcaatcacca
     3601 gccgacagca ctgatctttg gtcttctggt ggcaatatac gtgtgcatcc gtttaagtat
     3661 gacgggaggc aatggtgcca ccatggtggc actcgcggcg acggcaatgt tcattgcggt
     3721 gggcagcgtc ttctttctgc tgcccttcgt gctggaacgc gggatgacgg ccgatgattt
     3781 ttcagcagca tcgctgatca cctttgcctg gccctcggtc gcgacgctgc acgatttcgt
     3841 ggtctggggg agtgcaggcg ctggcccggt ttacgcggcc tatgtgggct tgccgatgct
     3901 gggctgcgtt gccgcgggcg gctacgcgat ttggacgaac tcccaaacgc ttgaacgctc
     3961 tctccgctca acatggtcgc tcatgctcgg tctggcgtcg ataagcctgt tcgtacgcgg
     4021 cgcttatgtg cgggaggcga catttacgtt tttcttcctc tgcgtggctg cgggggttgg
     4081 gctggagatg gcaacgcgcg ggcaccggcg cggaagtctg ctggcggcgg tgttcgtgac
     4141 aactttgttg gatgccggcc ccctcgcggt gcagccatgg acacgcagag attttgtgcc
     4201 aatcgccgaa gcgggcgaat accttgtgga tcgggcccgt aatgcgcggg tggtggaagt
     4261 gcagcaaatc gatggtaaag cctatatcgc agccgatccc acgctcagcc cactagcata
     4321 tggccgtttg cagatcctgg caggtccgca taagcaggat gcgaccaagg cgcataacgg
     4381 ttttgccgcg ctgttgaaga tcgctcaaca ggatctgcgt acagatggac ggttggagcc
     4441 gcggacccga aacatgcttg cagtcgcgaa cgtaggctgg gtggtcggca ctgccgccaa
     4501 agggatgggg ctgcctaaca acctcgaggg cacgatcaca gacccggtct tgggcactta
     4561 tctgcccatc ccagaggcta ctccgttcat cgttagcggt cggctcgaga ccatgacgcg
     4621 gccaatggcc ttcgatgtg
//
LOCUS       DASWQN010000428         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1756593, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000428 DASWQN010000000
VERSION     DASWQN010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756593"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..707
                     /locus_tag="VGC82_07045"
     CDS             210..707
                     /locus_tag="VGC82_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0423063.1"
                     /translation="MARALTWGADEKVLLILAAAGWLASRGCAEPLRRTGNHALLVTV
                     VASLLPHGLKLVFDQTRPDRRTVIGHLHGVPFSGKSQDAFPSGHALHMGALASAAGAL
                     PTGPRRAIRAFAVGLSLTRIVVLAHWASDVVAGFALGAVLERALRLWTGYLGGTSTED
                     DHANP"
     gene            769..1299
                     /locus_tag="VGC82_07050"
     CDS             769..1299
                     /locus_tag="VGC82_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0423064.1"
                     /translation="MDLARVRKSHKVRFKDDGLVPNHPRWPLIIYRKAVAFDDRQDPA
                     AVLEDLFEANGWGDTWRDGIYDYVHYHSRIHEALGVARGKGRVRFGGKKGRIFTLKAG
                     DVVILPAGTGHQCLSADDEFLVIGAYPPSGTYDECMTIEDRPRALKTIPKVPVPRKDP
                     VYGTGGPLSKLWKGAK"
     gene            1296..>1546
                     /locus_tag="VGC82_07055"
     CDS             1296..>1546
                     /locus_tag="VGC82_07055"
                     /inference="COORDINATES: protein motif:HMM:NF022789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HEY0423065.1"
                     /translation="MTEYVIRFLVGGAVVSAFAMLGDLLRPKSFAGLFGAAPSVALAT
                     LGIAVYQHGAGYAALQTHSMMAGAMALGIYSVVVCHLLIR"
BASE COUNT          266 a          479 c          496 g          305 t
ORIGIN      
        1 tgcgacagcg gggaaggact tttaacggcg gttcgcgcgt tggaaatgta ctttcctttg
       61 cgatcgttag aggcatgttc aaaatagcta gatcgtcagc aaagttcccc ggccgcggtc
      121 caagctttcc gatcaccgtc cggccaacca gagcagatgt cgccgtcgcg caatctatcg
      181 cccacaacac tgcgccggct ccggagcagg tggcccgcgc tctgacgtgg ggcgccgacg
      241 agaaggtcct tctgatcttg gcagccgccg gctggcttgc ctcgcgcggg tgcgctgagc
      301 ccctgcggcg caccggcaac catgctctgc tcgtcacggt cgtagcctcg ctgctgcccc
      361 atggtttgaa acttgtgttc gaccagaccc ggccggaccg ccgaacggtc atcgggcatc
      421 tccatggcgt tccgttttcc ggcaaaagtc aggatgcctt tccttccggc catgcgctgc
      481 acatgggagc cttggcatcg gccgccggcg cgttgccgac cggcccccgc cgggcgatcc
      541 gggcgtttgc ggtaggactg tcccttacgc gcatcgtggt tctggcgcac tgggcgagcg
      601 atgtcgtcgc gggcttcgca ctgggtgcgg ttctcgaacg ggccctccgg ctttggactg
      661 gttacctggg cggcacttca acggaggacg atcatgccaa cccttgaaga cctgaaggga
      721 tacgcggagc gcgcaaccgg tcttcgacgc cccggcaagc ggaaggccat ggacctcgcg
      781 cgagtgcgaa agtcccacaa ggtccgtttc aaggacgatg gtctcgtccc aaatcatccg
      841 cgctggccgc tgatcatcta tcgaaaagct gtcgcgttcg atgaccgcca agaccccgcg
      901 gccgtgctcg aagacctgtt cgaagcgaac ggctggggag atacctggcg cgacggcatc
      961 tacgattacg tgcactatca ctcccgaatc catgaagcgc ttggcgtcgc gcggggcaaa
     1021 ggtcgcgtcc ggttcggtgg caaaaagggg aggatcttca cgttgaaggc cggcgacgtg
     1081 gtcatcctgc ctgctggcac gggccaccag tgcctttcgg cggacgatga gtttctcgtc
     1141 atcggggcct acccgccgag cggcacatac gatgaatgca tgaccatcga ggaccgtccg
     1201 cgggcgctaa agaccattcc gaaggtgccg gtgccgcgca aggatccggt ctacgggact
     1261 ggcgggccgc tctcgaaact ctggaaggga gcgaaatgac ggagtatgtg atccgctttc
     1321 tcgtcggggg ggcggtcgta tcggccttcg cgatgctggg agatcttctg cgtccaaaga
     1381 gcttcgccgg cctgttcgga gccgcgccat cggtcgcact cgccacgctg ggtatcgccg
     1441 tgtatcagca tggtgccggc tatgcggcgc tacaaactca ttcgatgatg gcaggggcaa
     1501 tggcacttgg catctatagc gtggtggtct gtcatctcct cattcg
//
LOCUS       DASWQN010000429         3093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2548460, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000429 DASWQN010000000
VERSION     DASWQN010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2548460"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..953)
                     /locus_tag="VGC82_07060"
     CDS             complement(294..953)
                     /locus_tag="VGC82_07060"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY0423066.1"
                     /translation="MKLILLGPPGAGKGTQAKRLEEKYQIVQISTGDMLRAEVAAGSP
                     IGKEAKAVMEAGQLVSDDILVRMLGARLDQPDAARGFILDGFPRTVPQAQALDRMLAE
                     KNLKLNAVIELKVDDAALTDRIAGRFTCANCGRGYHDTFRPPAVAGACDACGSATFIR
                     RADDNRETVAARLEAYHRQTAPIIPHYAAAGILRTVDGMAEIQEVSRQIDAVLAGPLS
                     A"
     gene            complement(950..2287)
                     /gene="secY"
                     /locus_tag="VGC82_07065"
     CDS             complement(950..2287)
                     /gene="secY"
                     /locus_tag="VGC82_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY0423067.1"
                     /translation="MASAAEQLAANLNMGVFSKATELKQRIWFTLGALIVYRIGTYIP
                     VPGVDASVMGEMLSQHGGGILGMFDMFTGGALRRMTVFALNIMPYISASIIIQLMSAA
                     VPTLETLKKEGESGRKKLNQYTRYLTVVIAMFQSYGIAVGLETMHGSFGAAVIDPGWF
                     FRLSCMITLVGGTMFLMWLGEQITARGIGNGTSLIIMSGIVANLPTAFASMLEMGRTG
                     ALSPFFILLFMVIAVATIAIIVFVERAQRRIAVQYPKRQVGNRMFGGDSTHIPLKINT
                     SGVIPPIFASSILLIPATIAGFATNGPPWLQMIASQLAHGQPLYLALYAFMIIFFAYF
                     YTAVVFNPEETADNLKKYGGFIPGIRPGSATADYFDRILTRLTTVGGIYLVLICLLPE
                     ILISDYGLPFYFGGTSILIVVSVTMDTVTQIQSHLLAHQYEGLIRKTRAKGRR"
     gene            complement(2466..2954)
                     /gene="rplO"
                     /locus_tag="VGC82_07070"
     CDS             complement(2466..2954)
                     /gene="rplO"
                     /locus_tag="VGC82_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY0423068.1"
                     /translation="MNLNELRDNPGARLKSKRLGRGIGSGKGKTSGKGHKGQSAREGV
                     ALNGFEGGQLPIYRRLPKRGFHNLFRKEYAPVNLGALDAAIASGKVDGSQPITETTLI
                     AAGLASDDKDGIRLLAEGKISRAITITVSGASATARAAVEQAGGSVTTTVAPKAAPEA
                     SA"
     gene            complement(2956..>3093)
                     /gene="rpmD"
                     /locus_tag="VGC82_07075"
     CDS             complement(2956..>3093)
                     /gene="rpmD"
                     /locus_tag="VGC82_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY0423069.1"
                     /translation="KPGQKGTLVGLGLNKLRSTRDLEDTPSVRGMIRKVAHLIKVEEL
                     S"
BASE COUNT          613 a          970 c          929 g          581 t
ORIGIN      
        1 tacgccggca atacgcgcca cgctcgactc tcctgtcgtc cggtcatttc gatccggacc
       61 ttcaactgcc ccgaaggact gaattacgcg caagcggcgg caaaccccca tatggggttg
      121 cgccggagcg cgggactata ggttccgcgg cgcaggagtc aacgagcagt tatcaactct
      181 gcgtgagaac atcaacatat gtatctgggc tgggcatgat cacgttcggc tggacatccg
      241 aagcctgtga atcatgcgat taatcaaaaa gatggccagt gccgccggca ccgtcaagct
      301 gataatggtc ccgccagcac ggcgtcgatc tgacgggaaa cctcttggat ttcggccatg
      361 ccatccacgg tccggagaat cccggccgct gcatagtggg gaatgatcgg cgcggtctgc
      421 cgatggtagg cttccaggcg ggcagcgacg gtttcgcggt tatcgtccgc tcggcgaatg
      481 aaggtagccg aaccgcaggc atcgcatgca cccgccacgg cgggaggcct gaacgtgtcg
      541 tggtagccgc ggccgcaatt ggcgcaggta aaccggccgg cgatgcgatc ggtcagcgca
      601 gcgtcatcga ctttcagttc gatcaccgcg ttcaacttca aattcttctc ggcgagcatg
      661 cggtctagcg cttgcgcctg aggtacggtg cgtggaaatc cgtccaggat gaagcccctc
      721 gcggcatccg gctgatccag ccgggcgccc aacatgcgga ccaggatatc gtcggagacg
      781 agttgccctg cctccatgac cgcttttgct tccttgccga tcgggctacc cgcggcaacc
      841 tccgcgcgca gcatatcgcc cgtggaaatc tgcacgatct ggtacttttc ctccaggcgc
      901 ttggcctggg ttcccttgcc cgcgccgggc gggcccagaa gtatgagttt caccgacgcc
      961 ccttcgcccg agtctttcgg atcagacctt cgtattggtg agccagcaga tgagactgga
     1021 tctgcgtcac cgtgtccatc gtaaccgaca ccacgatcag gatcgaggtc ccgccaaaat
     1081 agaacggcag cccgtagtcg ctgatcagga tctccggcag cagacaaatc agcacgaggt
     1141 agatgccgcc gacagtcgtc aaccgggtca ggatgcggtc gaaataatcc gccgtcgcgc
     1201 tgcccggccg aatgcctggt atgaagccgc cgtatttctt caggttgtcg gcggtctcct
     1261 cggggttgaa gacgaccgcg gtgtagaaat acgcgaagaa aatgatcatg aacgcataca
     1321 gcgccagata gagcggctga ccatgcgcaa gctgcgaggc gatcatctgc aaccacggcg
     1381 ggccgttcgt cgcgaagccg gcgatcgtcg ccggaatcag caggatggag ctggcgaaaa
     1441 tcggcggaat gacacccgag gtgttgatct tcaacggaat atgggtggag tcaccgccga
     1501 acatccggtt cccgacctgg cgcttgggat actgcaccgc gatgcgccgc tgcgcccgct
     1561 ccacgaacac gatgatcgca atggtggcca cggcgatgac catgaacagc aggatgaaga
     1621 acggcgacaa cgcgccggtt cggcccatct ccagcatcga ggcgaacgcg gtcggcaggt
     1681 tggcgacgat cccggacatg atgataaggc tggtgccgtt gccgatgccc cgggcggtga
     1741 tctgttcgcc cagccacatc aggaacatgg tcccgccgac cagggtgatc atgcatgaca
     1801 gacggaagaa ccatcccgga tcgatcaccg ccgcgccgaa gctgccatgc atcgtctcca
     1861 gtcccacggc gatgccatag gactggaaca tggcgatcac gacggtcaaa tagcgggtgt
     1921 actgattgag cttcttgcgc ccggattcgc cttccttctt caatgtctca agggtcggaa
     1981 ccgccgccga catcaactgg ataatgatgc tggcgctgat atagggcatg atgttcagcg
     2041 cgaacacggt catgcgccgc agcgcgccgc cggtgaacat gtcgaacatg ccaagaatgc
     2101 cgccgccatg ttgcgacaac atctctccca tcacggaggc atcgacgccc ggcaccggga
     2161 tataggtgcc gatgcggtaa acgatcagcg cccccagcgt gaaccagatg cgctgcttga
     2221 gctcggtcgc cttcgaaaag acgcccatat tgaggttggc tgcaagctgt tcggcagcgg
     2281 aagccatgag tgttcccttt gtccacgccg tctaaatcga gtcgaacgtc aggttcgcgc
     2341 ggctggcttt agtctctatc cgagcggatc tggacgcaag gcggatccca atcaagccca
     2401 atcaagcgaa aagccacgca gggcaaaacg cctgcgtggc tttcgcttca tcggaccgtc
     2461 cgctcttagg ccgaggcttc cggagccgcc ttcggcgcaa ccgtcgtcgt cactgaaccg
     2521 ccggcctgct ccaccgcggc cctcgccgta gccgaagcac cggaaaccgt gatggtaatc
     2581 gctcggctga tcttgccctc cgccagcagc ctgatgccgt ctttgtcgtc cgacgccagg
     2641 ccggcggcga tcagcgtggt ttcggtgatc ggctgagaac catcgacctt gcccgatgcg
     2701 atcgccgcat cgagcgcgcc cagattaacc ggggcgtatt ccttgcggaa aagattgtgg
     2761 aaaccgcgct tcggcaaccg gcgatagatc ggcagctgac cgccttcgaa gccattcagc
     2821 gccacacctt cgcgggcact ctggcccttg tggcccttgc cggaggtttt acccttgccc
     2881 gatccaatcc cgcggccgag ccgcttggat ttcaggcgcg cgcccggatt gtcgcgcagc
     2941 tcattgaggt tcatgtcagg aaagctcctc caccttgatc aggtgggcga ccttgcggat
     3001 catcccgcgc acggaagggg tatcctccag gtcgcgcgtg ctccgcagtt tgttcagccc
     3061 cagcccgacc agggtaccct tctggccggg ttt
//
LOCUS       DASWQN010000430         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2548470, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000430 DASWQN010000000
VERSION     DASWQN010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2548470"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..168)
                     /locus_tag="VGC82_07080"
     tRNA            complement(93..168)
                     /locus_tag="VGC82_07080"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(133..135),aa:Lys,seq:ctt)
     gene            complement(234..>1661)
                     /locus_tag="VGC82_07085"
     CDS             complement(234..>1661)
                     /locus_tag="VGC82_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEY0423070.1"
                     /translation="GGGSSINGQLANRGAPTDYDEWHARGASGWNWDNVLPYFRKVER
                     DMDFEGPWHGNQGRIPVRRIFPDLWPEHAKAVGRAFEHAGWKFIQDQNAEWEDGYFPI
                     TISNAYERRVSAAIGYLDPGTRMRENLTISTDTQASGLIFDGQRCVGVNATVAGQPRE
                     FRGKEIILSCGAIHSPAHLLRAGIGPGAHLREHGIEVRASLPGVGQRLQDHPAVAVAA
                     FLKPHARIIHDYTRRHIYTALRYSSKLPGIPPGDMFTVVTNKTSWHAVGEQIGSFIVT
                     VYKTYSETGSVRLRSSSAADEPQVDFNLLSDQRDLERMMDGVRQFGAMHLTPIMQAVT
                     DNPFPASYSDKVRQVGLHSRKNKLITDVLAMALDGPAALRRYLIETLVMEGVSLQDVL
                     TDDEKLEAFVRKAAVGVWHASCTCRMGADTDPMAVTDPAGRVRGVEGLRVVDASVFPV
                     VPCANTNFPVLMTAEKMSDAILAGT"
BASE COUNT          302 a          547 c          508 g          304 t
ORIGIN      
        1 gaaaaggatg tccggcgggt ccgctggggg tccgcaaaag atctcttcgt ccggcagaca
       61 tcatgtctat atggacaagc cattgaaatc attggtgggc gcacaaggac tcgaaccttg
      121 ggcccgctga ttaagagtca gctgctctac caactgagct atgcgcccgc accaacgacg
      181 ggcggtttct accaacgttc ccccgcaatg caacccccca cacggaacgg ccgtcaagtg
      241 ccggccagga tcgcgtccga catcttctcc gccgtcatca gcacgggaaa gttggtgttc
      301 gcgcagggca cgaccggaaa caccgaggca tcgaccacgc gcagcccttc cacgccccgt
      361 acgcggcccg ccgggtctgt aacggccatc gggtcggtgt cggcccccat gcggcatgta
      421 cacgacgcgt gccacacccc cacggcagcc ttgcgcacga aagcctccag cttttcatcg
      481 tcggtcagca cgtcctgcaa tgagacgcct tccatcacca gggtctcgat caaataccgc
      541 cgcaacgccg ccggcccgtc caaagccatg gccagcacgt cggtgatcag cttgttcttg
      601 cggctgtgca gtccgacctg ccgcaccttg tcgctgtagc tggccgggaa agggttgtcg
      661 gtcacggcct gcatgatggg cgtcaggtgc attgcgccga actggcggac cccgtccatc
      721 atccgctcca gatcgcgctg gtccgagagc aggttgaagt ccacctgcgg ttcatctgcc
      781 gcactggatg aacgcagacg caccgaaccg gtttcggaat aggtcttgta cacggtaacg
      841 atgaacgacc cgatttgctc gcccaccgcg tgccacgacg tcttgttggt cacgacggtg
      901 aacatgtccc ccggcggaat gcccggcagc ttcgaggaat accgcagcgc cgtgtagata
      961 tgccggcgcg tgtagtcgtg gatgatccga gcatgcggtt tgagaaaggc ggcgaccgcg
     1021 accgccggat ggtcctgcag tcgctgcccc acacccggca gcgatgcccg tacctcgatg
     1081 ccatgttccc gcagatgtgc gcccggcccg atgccggccc gcagcaggtg cgccggggag
     1141 tgaatggcgc cgcaggacag gatgatttcc ttgccgcgaa attcccgcgg ctggccggcg
     1201 accgtcgcgt tcacgccgac acagcgctga ccgtcgaaga tcagcccgct ggcctgggtg
     1261 tcggtggaga tggtcagatt ctcccgcatc cgcgtcccgg gatcgaggta accgatcgcc
     1321 gcggatactc ggcgttcata ggcgttggag atggtaatcg ggaaataccc gtcttcccat
     1381 tcggcattct ggtcctggat gaacttccag ccggcgtgct cgaatgctcg gccgaccgcc
     1441 ttggcatgtt cgggccataa atccggaaat atgcggcgga cgggaatgcg gccctggttg
     1501 ccgtgccacg gaccttcgaa atccatgtcg cgttcgacct tcctgaaata cggcagcacg
     1561 ttgtcccagt tccagccgga cgcgccacgg gcgtgccatt cgtcgtagtc ggtcggtgcg
     1621 ccgcgattgg cgagctggcc gttgatcgac gagccgccgc c
//
LOCUS       DASWQN010000431         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1228989, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000431 DASWQN010000000
VERSION     DASWQN010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228989"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1178)
                     /locus_tag="VGC82_07090"
     CDS             complement(102..1178)
                     /locus_tag="VGC82_07090"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY0423071.1"
                     /translation="MPTHPAVTRAQAFCTAYDLRIPILLAPMAGACPPSLSTAIANAG
                     GLGACGCLLMQPDAIKGWASEVRAGSNGGFQLNLWIPDPPPPRDAIAEASVRAFLATW
                     GPEVPPQAADAAPPDFAAQCEALLEAGPSIVSSIMGVYPPEFVTRMKASGIRWFATVT
                     TVREALEAEAAGADAIVAQGMEAGGHRGTFDASQAGTSLAGLFSLLPAVVDAVNVPVI
                     ATGGIADARGVAAALILGASAVQIGTGFLRTPEAKLPAAWADAIGRSQPEDTLVTRAF
                     SGRLGRSVANAYARAATAPDAPPPAPYPVQRGLTQAMRDAATKAGDIGRMQAWAGQSA
                     RLAQPVPAGELARGLWEAASAMLP"
     gene            1655..2185
                     /locus_tag="VGC82_07095"
     CDS             1655..2185
                     /locus_tag="VGC82_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0423072.1"
                     /translation="MLPESFRTARLYLRPVTMADAGPIFAAYSRNPEVTLFLIWRPHR
                     SRRDIEAYIRSCLETSPHRARTYVMQGRKDSVIRGCFDLRRAERHRLEFGYVLARPWW
                     GQGLMTEALTEVVRWGLAQPAIFRISAVCDVENIASARVMEKAGLTKEGLLRRYLIHP
                     GISSEPRDCFSYAKVR"
BASE COUNT          396 a          753 c          843 g          442 t
ORIGIN      
        1 gaatgcgcat cagcaattcc tcgaccggac cggcaagcgg cgtgatcctc gcgtcgcttc
       61 ctgggccgcg gtatcaacgc attccattcg atcgggttgc gctacgggag catcgcgctt
      121 gccgcttccc acagcccacg cgcgagctct ccggcaggaa caggttgtgc cagtctggcg
      181 gactggccag cccaggcttg catgcggccg atgtccccgg ctttggtcgc tgcatcccgc
      241 atcgcctggg tcaggccgcg ttgcacggga tacggtgccg ggggcggggc gtccggtgcc
      301 gtcgcggcgc gggcataagc gtttgcgaca ctgcgcccga ggcggcccga gaatgcgcgc
      361 gtaaccaggg tgtcctcggg ttggctgcgg ccgatggcgt ccgcccaggc tgccggtagt
      421 tttgcttcgg gtgtgcgcag aaagccggtg ccgatctgga cggcggacgc ccccaggatc
      481 aaggcagcgg caacaccgcg ggcatcggcg atgccgccgg tggcgatcac cggcacgttc
      541 accgcgtcaa cgaccgcggg cagcagcgag aacagaccgg ccaggctggt cccggcttgg
      601 ctcgcatcga acgtgccgcg gtggccgccg gcctccatgc cctgggcgac gatcgcatcg
      661 gcgccggccg cctcggcttc aagcgcctcc cgcaccgtgg tgacggtggc gaaccatcgg
      721 ataccgcttg ccttcatccg ggtgacgaat tccggtggat aaacgcccat gatcgaggaa
      781 acgattgacg ggccagcctc cagaagcgcc tcgcattgcg cggcaaaatc cggtggtgcc
      841 gcgtcggcgg cctgcggcgg cacctccggt ccccaggtgg cgaggaaggc gcgtacgctg
      901 gcttcggcga ttgcgtcgcg cggtggaggc gggtccggaa tccaaagatt aagctggaaa
      961 ccgccattgc tgccggcgcg gacctccgag gcccagccct tgatcgcgtc cggttgcatc
     1021 agcaggcaac cgcacgcgcc gaggccaccc gcatttgcga tcgcggtgga cagggacggc
     1081 ggacaggcgc ccgccatcgg tgcgagaagg atcgggatgc gaaggtcgta ggcggtgcaa
     1141 aaggcctgtg ctcgggtcac ggctgggtgt gtcggcatcg ctgctcctcc gtttggcgga
     1201 agactacgat gtggtaggcg ggccggataa tgctttgatc ggatcgctgc tatctgtgga
     1261 agtggcgggt agggatttgg ttggcattcg tgcgcccggc cgtgccggcg ggtgcaactt
     1321 cgattcaggc acttgcggca gccccaggac acgcctcgca tgcgccagtt cggcttcttg
     1381 ctgttcggga ggcggcaatg gcgggggcag tccggcgagc cgcagcatcg cgatgacgtc
     1441 ccccgccggc ccggtcatcg agcagagagg cagcctggat tgctgaagtt ttgccgttcg
     1501 catagcggcg gatgacggga tgcacggtac ttggccggtg tgtcatcaag agctggcgtg
     1561 tcatcaagac aggtcacatg attcaagggg tcttgtctgg aaagggctat ggtcactgct
     1621 ttatgtccgc atcgaatcgc ggcatatgcg gtgaatgctt ccggaatctt tccgcacggc
     1681 gcggctttat cttcggcctg tcacgatggc ggatgccggc ccgatcttcg cggcctattc
     1741 gcggaacccc gaggtcacgc tgttcctgat ctggcggccg caccggtccc gccgcgacat
     1801 cgaagcctac attcgcagtt gcctcgagac ctcgccgcac cgggcgcgca cctatgtgat
     1861 gcagggccgc aaggatagcg tgatccgcgg ttgcttcgat ctgcgtcgag ccgagcggca
     1921 ccgactggag ttcggctacg tgctggctcg gccctggtgg ggccagggac tgatgacgga
     1981 agcattgacc gaggtcgtcc gctgggggct ggctcagcct gcgatcttcc gcatcagcgc
     2041 ggtatgcgat gtggagaaca tcgcttcggc ccgggttatg gaaaaggccg gtctgaccaa
     2101 agagggtctg ctgcggcgct atcttattca ccccggcata agcagtgagc cgcgggattg
     2161 cttcagctac gccaaggttc gctaaaccat ctgcggttcg gaaaccgtgt gccgaatcca
     2221 gtcggggccg tactggcacg aggcagtcga gttcggacta tgggtgcgtg accgcgagat
     2281 cccgaccatg gccctgctcg aatcgatgac caccgcgcca gccaccacgg aagtgcatta
     2341 ccgcatcggg ggaatcgccg aaacgttcat gtccacgctc ctgccgatgc tggtcgagca
     2401 ggggcgcatc gggtttgatg acaggatctc gcat
//
LOCUS       DASWQN010000432         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_437131, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000432 DASWQN010000000
VERSION     DASWQN010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437131"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VGC82_07100"
     CDS             <1..937
                     /locus_tag="VGC82_07100"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY0423073.1"
                     /translation="ILAGAAYLREMYERFGSPAFLAAYNAGPSRVDRYLNNGTPLPAE
                     TVNYVASIAPLLGTEMAMSGPLAVYAGGGGYAPRMAARATADGCDPDAAYNPAGPCTP
                     LRPAVVQTVAVATAPYKMQDCDPDAAYDPTRPCQPTSVVAQAAVARSVAIPPERFAAA
                     TGRPVQDCDPDTAYDPTRQCRPAPVVARPAITQPAATPPERFIAAARPLQERMAEPVA
                     FSAGPPPGRWMIQVGAFATLTIAEAAAERARSVAPDLLRFAKIELPATAPLGTQVAFR
                     ARLSGLSASAAADACARLNNRGTACITVPPRQGSF"
     gene            complement(1025..>2119)
                     /locus_tag="VGC82_07105"
     CDS             complement(1025..>2119)
                     /locus_tag="VGC82_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY0423074.1"
                     /translation="ISSLDTALFRIELPVPLSDSTHGTMTHFELVTVRIRDSDGAEGT
                     GYTYTTGAGGAAVHALIDQGLRPVLLGADADQIEALWSRMWWKLHYGGRGGSVSLAVS
                     ACDIALHDLKARRLGSPLWRMLGGFDASVPCYAGGIDLDFTMEALLKQTEDNLAKGFR
                     AIKMKVGRARLSEDRERVRAMRAHLGDDFPLMADANMRWTVDQAIAAARAFRDNNLTW
                     LEEPTIPDDIAGHARIVREGGVPIATGENLHTLHEFTQMIAAGGVTFPEPDVTNCGGV
                     TVFMKIAHLAEAFNLPLTSHGAHDLTVHLLAAVPNRSYLEVHGFGLDRYIQQPIAIAE
                     GNALAPDRPGHGVAFNWTELEKVRVAGNAG"
BASE COUNT          331 a          788 c          655 g          345 t
ORIGIN      
        1 catcctggcc ggcgccgcct atctgcggga aatgtacgag cggttcggct ccccggcctt
       61 tcttgccgcc tataacgccg gtccgtcgcg ggtggaccgg tacctgaaca acggcacgcc
      121 gctgcccgcc gagacggtca actatgttgc ctcgatcgcg cccctgctgg gcacggaaat
      181 ggcgatgtcc ggtcccttgg cggtgtatgc cggcggcgga ggctacgcgc cacgaatggc
      241 cgcccgtgcc accgcggacg gttgcgaccc ggacgcggcg tacaacccgg ctggcccatg
      301 cacgccgctt cgcccggccg tggtgcaaac cgttgccgtc gccacggcac catacaagat
      361 gcaggactgc gacccggacg ccgcctatga cccgacccgc ccgtgccagc caacgtcggt
      421 tgtcgcccag gcggccgtcg ctcgctcggt cgccattccg cctgagcgat ttgccgccgc
      481 cacgggacgg ccggtgcagg actgcgaccc cgacaccgca tacgacccaa cccggcagtg
      541 ccgaccggcg ccagtcgtcg cacgcccggc gatcacacag ccggccgcca ctccgcctga
      601 gcgatttatc gcggcggcac ggccgttgca ggagcggatg gccgaaccgg tcgccttctc
      661 cgccggtcca ccgcctggcc gctggatgat acaggtcggc gccttcgcca ccctgacgat
      721 tgccgaggcg gccgcggagc gtgcccggtc ggtcgcaccg gatttgctgc ggttcgcgaa
      781 gatcgaattg ccggcgaccg cgcccctcgg cacccaggtt gcctttcgcg cgcggttgtc
      841 cggtctgtcc gcctcggccg cggcggacgc ctgcgcccgg ctaaacaacc gaggcacggc
      901 ctgcatcacc gtgccgccac ggcaaggttc gttctgatcc gttgatcgaa ggctcgggcg
      961 gccagggtcc tccacgcgcc ggtgtcctcc acgcgccagt gtcctccacg cgcgatccag
     1021 acgcctaacc cgcgttgccg gcgacccgaa ccttctccag ttcggtccag ttgaacgcta
     1081 cgccgtgccc aggccggtcc ggcgccagcg cgtttccctc ggcgatcgcg attggctgct
     1141 ggatgtagcg gtccagtccg aacccatgca cctccagata cgaccggttc ggcaccgccg
     1201 cgagcaggtg caccgtcaga tcatgcgccc catgcgaggt cagcggcaga ttgaacgcct
     1261 ccgccagatg ggcgattttc atgaacaccg tcacgccgcc gcaattggtc acatccggct
     1321 ccggaaacgt cactccgccg gccgcgatca tctgggtgaa ctcatggagc gtatgcaggt
     1381 tctccccggt ggcgatcggc acgccaccct cccggacgat cctggcatga ccggcgatgt
     1441 cgtcggggat tgtgggttct tccagccagg tcaggttatt gtcgcggaac gcccgtgccg
     1501 cggcgatcgc ctggtccacc gtccatcgca tgttggcatc cgccatcaac ggaaaatcat
     1561 cccccagatg ggcccgcatc gcccgcaccc gctcgcggtc ctccgacagc cgcgcacgtc
     1621 ccaccttcat tttgatcgcc cggaatccct tggccagatt gtcttcggtc tgcttcagca
     1681 gcgcctccat cgtgaagtcc aggtcgatcc cgccggcata gcacggcacc gaggcgtcaa
     1741 agccgccgag catccgccaa agcggcgaac ccagtcgccg agccttcagg tcgtgcaggg
     1801 cgatgtcgca ggcggaaacc gcgaggctga cggaaccgcc gcgcccgccg tagtgcagct
     1861 tccaccacat ccggctccac agcgcctcga tctggtccgc atccgcgccg agcagcaccg
     1921 gacgcaatcc ctggtcgatc agcgcgtgca ccgccgcccc gcccgcgccg gtggtgtagg
     1981 tgtagccggt gccctccgcg ccatcgctgt cgcgaattct gactgtcacc agttcgaaat
     2041 gcgtcatcgt gccatgggtc gaatccgaca gcggcacggg aagttcgatg cgaaacagcg
     2101 cggtatccag cgaggaaat
//
LOCUS       DASWQN010000433         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_173354, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000433 DASWQN010000000
VERSION     DASWQN010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173354"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VGC82_07110"
     CDS             complement(<1..248)
                     /locus_tag="VGC82_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0423075.1"
                     /translation="MAQQIPVEDEALFGFEVPEEAVREVAPDLAYRRLAMVNVAFFGR
                     PGGDWVLIDAGLPATAGMIASSASARFGADRPPKAIVLT"
     gene            499..1500
                     /locus_tag="VGC82_07115"
     CDS             499..1500
                     /locus_tag="VGC82_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0423076.1"
                     /translation="MWEKRQLGRTRLRVTSFGLGTATLGGSRIPITQAEGQAIVTAAW
                     DAGLRYVDTAPFYGVGAAEHRVGDALRDKPRDEWVLSTKVGRLLRPKTDTSPSPDGRL
                     SPMPFKVLYEYSYDGIMRSVEDSYQRLGLARIDILYVHDIGVYQHGPELNAQYLTQLR
                     DSGYKALDELRRTGVVSAIGIGVNEKEVLIQALGFGDWDAFLLAGRYTLLEQGPLDDL
                     IPMCQSAGTSLVIGGPLNSGILAGRDTWNYEAAPPEIVAKVRKIAAVCAAHNVPLAAA
                     ALQFPLAHPIVACVIPGPRNVVEFRENLPLFTMKIPPGLWSDLRNEKLLHPNAPVPG"
     gene            1744..2649
                     /locus_tag="VGC82_07120"
     CDS             1744..2649
                     /locus_tag="VGC82_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423077.1"
                     /translation="MPIYQTDPWRVQFFAQAGCPDDVIIPTKDWEAWPLYPAHRWIYD
                     KLAVALSQGLDAAPHGVMPPGFPVFSKPMMNLRSMGAGSLAIPDLAGYQAALNPGHFW
                     CTLLTGAHVSTDAAIVDGNAVWWRHATGATAPGGTFDYWHIHAEAMPEIEAWCGAWAR
                     KNLAGYTGMVNFETIGSRIIEAHLRFADQWPDLYGPGWVDALVRLYGDGKWTFDDRDR
                     TDGYSVVLFGPHGPAYRHPPERQVAEARRIDGVSSVQVSFHEDRDPALHSMPPGGFRL
                     AIVNAIDLSAGQRARELLKASLFTM"
     gene            2707..>3617
                     /locus_tag="VGC82_07125"
     CDS             2707..>3617
                     /locus_tag="VGC82_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0423078.1"
                     /translation="MADESNLTARRPTAVAPAETPGLTGLLTLAVAVVVVAGLYLGRT
                     VLIPITLAVLLSFLLAPIVGVLRRIHFGRVLSVLVAVVLALSVILALGGLIGSQIADL
                     VQEVPKYASTVRGKVEAVQGFALTRMNLVMRRLEPQAGTSNATQPKQPASAEPKPMPV
                     EVHQPDPTPVELAERVIAPIVDPLSTTAIVLIVTVFVLLQREDLRDRLIRLFGSSDLH
                     RTTVAMNDAARRLSRYFLIQLGINAAFGIITGIGLLLIGVPSPLLWGTIGLLLRFVPY
                     IGAPLAALLPLALAAAVSPDWSMMLWTA"
BASE COUNT          590 a         1183 c         1191 g          653 t
ORIGIN      
        1 gtcaatacga tcgccttcgg cggccggtcc gcgccgaagc gtgccgaggc ggaactcgcg
       61 atcatgccgg ccgtggcagg caagccggcg tcgattaaca cccagtcccc gccaggacgg
      121 ccgaaaaagg ccacgttgac catcgcaagc cggcggtaag ccaggtccgg cgcgacttcg
      181 cgcactgctt cctccggcac ctcgaagccg aacaatgcct catcttcgac cggtatctgc
      241 tgcgccatga tcgggtctct cccgcagagt atcctggcaa catcggacaa ggggtcgcgg
      301 ttgagggtgc ggcggttcag aaccgcgtgt gaacgccgcc gcccaggcac gatcggctgg
      361 cgaggctggg gcgaatcctg tccaccttcc cggcgatttg tgcttcgcgg cagccgaggg
      421 catatcgggt tgcacgaagc gccgaagccc cgcactgtcg ccggcacgaa gaccggcacg
      481 ggagaaagga gccatccgat gtgggaaaag cgccaacttg gccgcaccag gctgcgggtt
      541 accagctttg gcctcggcac cgccacgctg ggcggcagcc gcatcccgat cacccaggcg
      601 gaaggccagg cgatcgtcac cgcggcctgg gacgccggcc tgcgctacgt cgataccgca
      661 ccgttctatg gcgtgggggc ggcggaacac cgggtgggcg atgcgctgcg cgacaagcca
      721 cgcgacgaat gggtcctgtc caccaaggtt ggccggttgt tgcggccaaa gaccgatacc
      781 tcgccatccc ccgacgggcg cctgtcgccg atgccgttca aggtgctgta cgagtacagc
      841 tacgacggca tcatgcggtc ggtcgaagac agctaccagc gcctcggcct tgcccggatc
      901 gacattcttt atgttcacga catcggcgtt taccagcatg ggccggagct caatgcccaa
      961 tacctgacac agctgcgtga cagcggctac aaagcgctgg acgaactccg ccgcaccggc
     1021 gtcgtcagtg ccatcggcat tggtgtcaat gaaaaggagg tgctgattca ggcgctcggc
     1081 ttcggcgact gggacgcatt cctgctggct ggccgttata cgctgctgga gcagggaccg
     1141 ctcgacgacc tgatcccgat gtgccagtcg gcgggcacct ccctggtcat cggcgggcct
     1201 ttgaactccg gtatcctggc tggccgcgac acctggaact acgaggccgc accgccggag
     1261 atcgtcgcca aggtgcggaa gatcgccgcc gtctgcgctg cgcataatgt gccgttggcg
     1321 gcggcggcgc tgcaatttcc gctggcccat cccattgtcg cctgcgtcat tccgggtccc
     1381 cggaacgtgg tggagttccg cgagaaccta ccgctgttca cgatgaagat cccgcccggc
     1441 ctgtggtccg atttgcgcaa cgagaagctg ctgcatccga atgcgccggt tccgggatag
     1501 gttgccaccg tggtctgggg cggctctggg tgtctgtgtc ggccggccgt agagccgtcg
     1561 ttgctcgttg tgtccggcct ggcgttgtgc cggccgcccg cgtgtgtccc gccctcaagc
     1621 aagtcgcggg tggccgcgcc aagcacggcc atgacacgat catcagcccg gcgtggcggt
     1681 gacacgatca ccaatcgcag tcaaacgatg aaccacgtct taagcagatg aaggagttcg
     1741 gcgatgccga tctaccagac cgacccgtgg cgggtgcagt tcttcgcgca ggcaggctgc
     1801 ccggatgacg tgattatccc gaccaaggat tgggaagcct ggccgcttta tcccgcccac
     1861 cgctggatct acgacaagtt ggccgtggca ctgtcgcaag gtttggacgc cgcgccgcat
     1921 ggcgtgatgc cgccggggtt cccggtgttc tccaagccaa tgatgaacct gcgcagcatg
     1981 ggcgccggca gcttggcgat ccccgacctg gcgggctacc aggcggcgct gaacccggga
     2041 catttctggt gcacgctgct gaccggcgcg catgtcagca ccgacgcggc cattgtcgat
     2101 ggcaatgcgg tctggtggcg ccatgccacc ggcgcgacag cgcccggcgg caccttcgac
     2161 tactggcaca tccatgccga agccatgccc gagatcgagg cgtggtgtgg cgcctgggcg
     2221 cgcaagaacc tggctggata caccggcatg gtgaatttcg agacgatcgg cagccgcatc
     2281 atcgaggcgc atttgcgttt cgccgaccag tggcccgatc tttacgggcc cggctgggtc
     2341 gatgccctgg tccggctgta cggcgacggc aagtggacgt tcgacgaccg cgaccgaacg
     2401 gacggataca gcgtcgtact gttcggaccg cacgggccgg cataccggca tccgcccgaa
     2461 cggcaggtgg cagaggcgcg gcggattgac ggcgtttcca gcgtgcaggt ctcgttccac
     2521 gaggatcgcg acccggcgct tcactccatg ccgcctggcg gctttcgcct tgcgatcgtc
     2581 aacgccatcg acctgtcggc tggacagcgt gcccgggagc ttctgaaagc gagcctgttc
     2641 acaatgtgat ccgaacgacg acgctattta ttgcattaac ccctcgttat gtacgaggcg
     2701 gaacgcatgg ccgacgagag caacttgacc gcgcggcggc cgacggcagt ggcgcctgca
     2761 gaaacccccg gcctgacggg attgctgacg cttgccgtcg cggtcgtggt ggtcgccggt
     2821 ctttacctcg gccgtaccgt gctgatcccg attaccctgg cggtgctgct gtccttcctg
     2881 ctcgcgccga tcgtcggcgt gctgcgccgc attcatttcg ggcgggtgct gtcggtactg
     2941 gtcgccgtcg tgttggcgtt gagcgttatt ctggcacttg gcgggctgat cggctcgcag
     3001 atcgccgacc tggtgcagga ggtgccgaaa tacgccagca ccgtccgcgg caaggtcgaa
     3061 gcggtgcagg gctttgccct gacccggatg aacctggtga tgcgtcgcct ggagccgcaa
     3121 gccggcacgt cgaacgccac gcaacccaag cagcccgcgt cggctgaacc caaaccaatg
     3181 ccggtggaag tccaccagcc ggacccgacg ccggtcgaac tggcggaacg cgtcatcgcg
     3241 ccgatcgtcg atccgctgtc cacgaccgct atcgtgctca tcgtgaccgt cttcgtgctg
     3301 ctgcaacgcg aggacctgcg cgaccgcctg atccgcctgt tcggatcgtc cgacctgcac
     3361 cgcacgactg tcgcgatgaa tgacgccgcg cgccggctgt cgcggtattt cctgatccag
     3421 cttgggatca acgcggcttt cgggatcatc accggcattg ggttgttgct gatcggcgtg
     3481 ccgtcgccac tgctatgggg cacgattggg ttgctgctgc gcttcgtgcc ctatatcggg
     3541 gcgccgttgg ccgcactgct gcccctggcg ttggctgccg cggtcagccc ggactggtcg
     3601 atgatgctgt ggaccgc
//
LOCUS       DASWQN010000434         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_833566, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000434 DASWQN010000000
VERSION     DASWQN010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833566"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VGC82_07130"
     CDS             complement(<1..533)
                     /locus_tag="VGC82_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0423079.1"
                     /translation="MPDYDLVVIGAGAAGLSVVAGAAQLGVSVALIERGVMGGDCLNT
                     GCVPSKALLAASHAVRAIRDAARFGITAGEPMVDWDRVRAHVHGVIASIAPVDSESRF
                     RALGATVLRGEAKFTGPATISVDGRAITARRFVIAAGSRATVPPINGLDRVAYWTNHS
                     LFDLTEKPDHLLILGGGP"
     gene            complement(526..1209)
                     /locus_tag="VGC82_07135"
     CDS             complement(526..1209)
                     /locus_tag="VGC82_07135"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY0423080.1"
                     /translation="MRRFLPLVLLVVLAAAGFFLMRGVSWESVGRHQGALMAWVGEHP
                     FNSAALYVVAYVLTAALSLPQASLLTVVGGLLFGSLLGCALAITGATIGASILLLVVR
                     SAFAQTLERQRHRIPESMQARLARDGFSYLLALRLLPLFPFWVVNLAAVVAGIPLVVF
                     MPATLLGIAPASFVFRSIGSGVGTILAEGRTPDLSVLFSAKILLPLAGLAVLSLLPAL
                     LRRRPGAHA"
     gene            1325..2293
                     /gene="trxB"
                     /locus_tag="VGC82_07140"
     CDS             1325..2293
                     /gene="trxB"
                     /locus_tag="VGC82_07140"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY0423081.1"
                     /translation="MSQTHHTKVLIIGAGPAGYTAAIYAARANLSPILVAGMQPGGQL
                     MITTDVENYPGFADVIQGPWLMEQMEAQATHVGTHIIHDLITEVDFSARPFHCQGDSG
                     DTYIASSVIIATGAQARWLGLDSEQALQGRGVSACATCDGFFYRGKSVAVVGGGNTAV
                     EEALYLTHHAEKVTLIHRRDALRAEKILQARLFAHPKIEVIWNSTVKEIVGGGTPEVV
                     IGVRLHNENTAEETVLPVDGVFIAIGHNPTTTVFAGHIDMDPEGYIRTTPGSTCTSVA
                     GVFASGDVQDKIFRQAVTAAGTGCMAALEAEKYLAEHEIRVSEAAE"
BASE COUNT          501 a          774 c          740 g          343 t
ORIGIN      
        1 ggtccgccgc cgaggatcag caaatggtcc ggcttctctg tcagatcgaa gagcgagtgg
       61 ttggtccaat aggcaactcg atccagcccg ttgatcggcg gcacggtcgc gcggctgccg
      121 gccgcgatga cgaaccggcg cgcggtgatg gcgcgcccgt ccaccgaaat ggtcgccgga
      181 ccagtgaatt tggcttcgcc gcgcagcacc gtcgccccca gggcgcggaa ccgcgactct
      241 gagtccaccg gcgcgatgga agcgataacg ccgtgcacat gcgcgcgcac gcgatcccaa
      301 tcgaccatcg gctccccagc cgtgatgccg aaccgcgcag catcccgaat tgcccgaacc
      361 gcatgcgaag ccgccagcaa cgccttgctc ggtacgcagc cggtattcag acagtcgcca
      421 cccatgactc cgcgctcgat cagtgcgacg gagacaccga gttgcgcggc accggcgaca
      481 acagaaagtc ccgcggctcc ggcaccgatg acaacaaggt cgtagtcagg catgcgcacc
      541 cggccggcgg cgcagcagcg cgggcaagag cgacagcacc gcgagcccgg ccaatggcag
      601 caatatcttt gccgaaaaca gcaccgacag atcgggcgtc cggccctcgg cgagaatagt
      661 gccgacgccc gagccaatgg acctgaacac gaaactggcg ggggcgatgc ctagaagagt
      721 cgcgggcatg aagacgacca gcgggatgcc ggccacgacg gcggcgaggt tgaccaccca
      781 gaacggaaac aacggcaaca accgcaatgc cagcaggtag ctgaatccgt cccgtgccag
      841 ccgcgcctgc atcgactcgg gaatacggtg gcgctggcgc tccaacgttt gcgcgaaggc
      901 agaccggacg accagcaaca ggatcgacgc accgatcgtg gccccggtga tcgccaatgc
      961 gcagcccaga agcgaaccaa acaacaagcc gccgacgacc gtcagcaggc tggcctgcgg
     1021 tagcgacagt gcggcggtta gaacgtaggc aacgacatag agcgcggcgg agttaaatgg
     1081 atgttccccg acccaggcca tcagcgcgcc ttgatggcgg cccacgcttt cccagcttac
     1141 cccgcgcatc aggaagaacc cggcggcggc gagaacgaca agcaacacca aaggcagaaa
     1201 acggcgcatg acgcgacaat ggcgtcgaac cgtcgcgttg tcgcctggtt tccggtgtcg
     1261 gccgtcgggg gactggcggt tgtcctcgct tgccgcgggc gctcgcacac cgatatgttt
     1321 gaccatgagc caaacgcatc acaccaaagt cctgatcatt ggcgccgggc cagccggcta
     1381 caccgccgcg atctacgcgg cgcgcgccaa cctgtcacct atcctggtgg ccgggatgca
     1441 gccggggggt cagctcatga tcaccaccga cgtggaaaac tatccgggct tcgccgacgt
     1501 catccaaggc ccgtggctca tggagcaaat ggaggcccag gccacccatg tcggtaccca
     1561 tattattcac gacctgatca ccgaggtcga tttctccgcc aggccgtttc actgccaggg
     1621 cgactcgggg gacacctaca tcgcctccag tgtcatcatc gccaccggcg cgcaggcccg
     1681 ctggcttgga ctggactcgg agcaggccct gcaaggccgc ggggtttccg cctgtgccac
     1741 ctgcgacggc ttcttctacc ggggcaagag cgtcgcggtc gtcggcggcg gcaatacggc
     1801 ggtggaggaa gcgctttacc tgacgcatca tgcggaaaag gtcactctga tccatcgccg
     1861 cgacgcgctt cgcgccgaaa aaatcctcca ggctcggctt ttcgcgcacc cgaagatcga
     1921 ggtgatctgg aacagcacgg tgaaggagat cgtcggcggc ggcacgccgg aggtcgtcat
     1981 cggcgtccgg ctgcacaacg aaaacaccgc cgaggaaacc gtccttccag tggacggcgt
     2041 tttcatcgcc atcggccata atccgacgac cacggtgttc gccggccata tcgacatgga
     2101 tccggaagga tatatccgca ccaccccggg cagcacctgt acctcggtcg ccggggtgtt
     2161 cgcgtcgggc gatgtgcagg acaaaatctt ccgccaagcc gtcaccgctg ccggaaccgg
     2221 gtgcatggcg gcactggagg cggagaaata ccttgccgaa cacgaaatac gggtctcgga
     2281 ggcggcggag taagcgaaat ttgctgaaaa acagcaaaat ccgtcacatg actcgcctaa
     2341 gtaggtttag ggtcgaag
//
LOCUS       DASWQN010000435         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1889217, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000435 DASWQN010000000
VERSION     DASWQN010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889217"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VGC82_07145"
     CDS             complement(<1..344)
                     /locus_tag="VGC82_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423082.1"
                     /translation="MKRKQQKIWAQAVPRPVCPDTLEKQALIAACEALITDVLKPRFQ
                     PTITPTEFNYPIDIRGDWRAGRYRFLRLYRFGHSTNEGMEFDAPFARIDRIGSDRFDI
                     NWMRHIGTWYRV"
     gene            complement(544..1434)
                     /locus_tag="VGC82_07150"
     CDS             complement(544..1434)
                     /locus_tag="VGC82_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY0423083.1"
                     /translation="MRQNSTGPVETTIVPRAHDVGGFEVHRALPAKEKQMVGPFIFFD
                     QMGPGEFLAGKGLDVRPHPHIGLATVTYLFDGSILHRDSLGTQLPIVPGDVNWMTAGC
                     GIAHSERTDANLRTHPNPLFGIQSWAALPKAMEETPPAFAHHEAADLPVLQDDGVRLR
                     LIAGDGWGCRSPVLTHWPLFYADAMLAPGGAIPMPDHHEERGAYIVTGSVDVAGTNFE
                     AGRMLLFRSGDRLSLRAGLQGARLLLLGGAVMDGPRYIFWNFVSSSRDRIEQAKADWK
                     ARRFGTVAGDDKEFIPVPER"
     gene            1530..>1803
                     /locus_tag="VGC82_07155"
     CDS             1530..>1803
                     /locus_tag="VGC82_07155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY0423084.1"
                     /translation="MTNVIGISGSLRNGSFNSALLRAAQALMPEGTRLTIGTIKGIPL
                     YNGDAEAADGVPPVVAALKDAVAAADGLLLATPEYNNSIPGVFKNAI"
BASE COUNT          326 a          545 c          588 g          344 t
ORIGIN      
        1 tgcacgcggt accaggtgcc gatgtgccgc atccaattga tgtcgaaacg gtcggaccct
       61 atgcggtcaa tgcgggcaaa cggagcatcg aattccatgc cttcgttagt tgaatgccca
      121 aaacggtata gccgtaaaaa ccggtaacgt cccgcgcgcc agtcgccacg aatatcgatg
      181 gggtaattga attcggttgg cgtgatcgtc ggctggaagc gcggtttcag aacatcggta
      241 atgagcgctt cgcaagcagc gatgagcgcc tgcttttcca gcgtatccgg gcataccggg
      301 cgcggcacgg cttgtgccca tattttctgc tgcttgcgtt tcattcgccg ctccgctgtc
      361 cggggttcct gtgccttgat cgccgacggt cgtccgtaag gaaccagcat tccctgccct
      421 gactggccgc ccctgtttcc tgcacggctt ccgcctggtc aatccgctgt gccgccctga
      481 caggtgatgc agtgtgggcg gaagcagtga gggcggaagc agtgtgggcc ttcgcggggc
      541 gcgctatcgc tccggcactg ggatgaattc cttatcgtcg ccggcgacgg tcccgaagcg
      601 gcgggccttc cagtcggcct tggcctgttc gatcctgtcg cgcgacgaag aaacgaagtt
      661 ccagaagatg tagcgtggcc cgtccatcac ggcgccgccc aatagcagaa gccgggcgcc
      721 ctggagtcca gcgcgcaagg acagacgatc gccggaacgg aacaatagca tgcgtccggc
      781 ctcgaagttc gtcccggcaa cgtccaccga accggttacg atatacgcac cacgttcctc
      841 gtggtggtcc ggcatcggta tggcgccgcc gggtgcgagc atggcgtcgg cgtaaaacag
      901 tggccagtgg gtcaggaccg gcgaacggca cccccagccg tcaccggcga tcaggcgcaa
      961 tcggacaccg tcgtcctgca gcacaggcag gtccgccgct tcgtgatggg cgaaggcggg
     1021 aggcgtttct tccattgctt tcggcaacgc ggcccaggac tggatgccaa aaagcggatt
     1081 ggggtgcgtg cgcaggttgg catcggtgcg ctccgagtgg gcgatgccgc atcccgccgt
     1141 catccagttc acgtcgcccg ggacgatggg caactgggtg cccaggctgt cgcggtgcag
     1201 gatcgagccg tcgaacaggt atgtaacggt ggccaggccg atatggggat gcggccggac
     1261 gtccagtccc ttgccggcca ggaactcccc aggtcccatc tggtcgaaaa aaatgaaagg
     1321 accgaccatc tgcttttctt tggccggcag cgctcgatgc acctcgaagc cgccgacatc
     1381 gtgggccctc gggacgatgg tcgtttccac cggaccggtt gaattttgcc tcatgggttt
     1441 cgcctttctc cagttcggcg ccacattgcg gtacatgccg ccggttgcgc tacttcaggc
     1501 ggcaacgcca cgacgatcag gggaacagga tgaccaacgt gatcggaatt tccggcagct
     1561 tgcgaaacgg gtcgttcaat agcgccttgc tgcgcgctgc ccaggcgctg atgcccgagg
     1621 ggacgcgact gaccattggc accatcaagg gcattccgtt gtacaatggg gatgcggagg
     1681 cggcggatgg ggtgccgccg gtggttgcgg ccctgaagga cgctgtcgcg gccgcggatg
     1741 gtttgctgct ggcaaccccc gagtacaaca actcgatacc gggcgtgttc aagaacgcga
     1801 tcg
//
LOCUS       DASWQN010000436         8039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_42067, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000436 DASWQN010000000
VERSION     DASWQN010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42067"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..451)
                     /locus_tag="VGC82_07160"
     CDS             complement(158..451)
                     /locus_tag="VGC82_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423085.1"
                     /translation="MKAAPDAFIARWEAREGGQERANYALFLSEVCDVIGVPRPDPSA
                     RATDNDYVIERAVKRQNPDGQLRRWPYHDNFVLGATTCDRCASYAHRGGFDGV"
     gene            908..1900
                     /locus_tag="VGC82_07165"
     CDS             908..1900
                     /locus_tag="VGC82_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0423086.1"
                     /translation="MDDRIDTWLAACPDPATDPLPGMREAGLLTPAPDYATVARMKAA
                     LVERTGSLGIASVWGGRQLVFRHFLSFGTDAQRAEWQGRSLAVAISEPKVGAHPKLLT
                     TRAAAASGEYHIAGQKAWVSNGPSADAIIVFAITAEDAGRKRYSAFIVPRDTPGLTMA
                     DMPGFHALRPSRHCLLTLDCVIPTSARLGSPGSAYERMALPFRDIEDAVGTFATLGAF
                     RYAAGLFAGQPMDQAEDLGAIVALTAVFAAAAEVVVAALDSGSFRTGNATLVGLRVLA
                     LDIVARLRALPGNALFTTILADLDAVLSIARGARLARQAHLGEAALRPVAARPE"
     gene            1897..2463
                     /locus_tag="VGC82_07170"
     CDS             1897..2463
                     /locus_tag="VGC82_07170"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY0423087.1"
                     /translation="MTVSRCHWAETSDAVMQAYHDHEWGVPQHDDRALFELLTLEGAQ
                     AGLSWRTVLVRRNAYRQAYHDFEIERVAAMTDEELSRLLSDSPLIRNRLKIYSVRDNA
                     RASLAAIAQHGSLDHYLWSFVGHTSIRNNPTDRFQVPATTPVSDAMSKALKKAGFRFV
                     GSTICYAFMQATGMVDDHLTSCYRHPQE"
     gene            2467..3885
                     /locus_tag="VGC82_07175"
     CDS             2467..3885
                     /locus_tag="VGC82_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY0423088.1"
                     /translation="MQNTEEVWRLVDAHQEDFIQLSDRVWEMPELCYAEFRSCAEHTA
                     MLEAKGFRVTTEVAGIPTAVMGEAGEDGPVIAILGEYDALPGLSQEAGVAQQKPLPGA
                     GFGHGCGHNMLGSASMLAATAVKDYLEANGIPGRVRYYGCPAEEGGGAKGFMARAGAF
                     SDVDIAISWHPSSFSGVNPANSLANTRIDFSFHGRASHAAASPHLGRSALDAVELMSV
                     GVNYMREHMPSDARVHAAILDAGGVAPNVVQAFAKVRYLVRAATLPELSSLIARVRKI
                     ADGAALMTETRVTAQVVSAVSNLLANTPLERAMQDNLERIGPPPFDEADRTAAREFQA
                     TLTDEDIEASYRRAGIPVRPDTPLCDVIIPLDAKPSGGVGSTDVGDVSWVVPTVQARG
                     ATYAIGTPGHSWQLTAQGKMPAAHKGLVHVAKVMAGTALDAIRDETLLARAKADHQAR
                     VGKTPYVCPLPDELDPPIKMSA"
     gene            3934..5088
                     /locus_tag="VGC82_07180"
     CDS             3934..5088
                     /locus_tag="VGC82_07180"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEY0423089.1"
                     /translation="MDTPLCRMLGVAFPLVAFSHCRDVVASVSRSGGFGVFGAAGCTP
                     EQLETELAWIDAHVDGHAYGVDLLVPENLSIRTAAGATQGDLVARVPDGHRAFVHDLL
                     RRHDVDPTRGSYDREDPNRPPSYQFETGERLMDVAFRHPIRLIANALGVPPASMLERA
                     RRHGIPCAALVGAREHALRQVAAGVDIIVAQGGEAGGHCGEVSTLVLVPEVLRAVQGA
                     RGQGIVPVLAAGGIMTGRQMAGCMAMGAAGAWTGSVWLATTEAETSPIFREKMLAARS
                     RDTVRSRGRTGKPARQLRSAWTDAWEAPDSPGALPMPLQGLISEPAMARINRAAEAGN
                     ANARQLVSYFVGQGVGLVDTIPTCRQVVAAFMTEFAEALDDMQRLSEAGG"
     gene            5090..5380
                     /locus_tag="VGC82_07185"
     CDS             5090..5380
                     /locus_tag="VGC82_07185"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY0423090.1"
                     /translation="MVKRLTDAERSTLGTSVPGWVQAPDRDAIRREFKFADFNEAWGF
                     MCRVALLAEKQDHHPDWSNVWNTVRIELSTHDVGGLSDNDVRLAQAINKILG"
     gene            5380..5850
                     /locus_tag="VGC82_07190"
     CDS             5380..5850
                     /locus_tag="VGC82_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEY0423091.1"
                     /translation="MDGIGFPERTSLVRKAAARLCLRLGWSPLHEVRLANGRRADILA
                     LQPGGGFVCIEVKSGLRDYLSDEKWTDYQPFCDQFFFAVDCDFPNDILPPETGLIVAA
                     GREADVLREPLAVRLSAPRRKALLERFAWLAAARLAALEDPMGVAELALGLSED"
     gene            complement(5861..7090)
                     /locus_tag="VGC82_07195"
     CDS             complement(5861..7090)
                     /locus_tag="VGC82_07195"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0423092.1"
                     /translation="MLDLAPVKQSDAIEDPRQSFLAGIDDLLPTIRARAGETERLGRV
                     PEDIVQRLTDAGVFRAAQPRQWGGLELDLATFYEGMIRIASACGSTGWVASVVGIHPW
                     QIALFANEAQRDVWATDPDARASSSYAPTGSVRREGDGFHLSGRWHFSSGVDHCGWAL
                     LGAVVPEDADGGAEFRTFLVPRRDFAIDQESWSVTGLGGTGSKNVVVRGAFVPEHRTH
                     RIVDVYRGTDPGFAVNDRPYYRLPWRLVFATTIAAPAIGAAAGALDAFIEQNQSRVSA
                     YGGLPVAQNPALHRPLARALTEVDAARTRMHAIWSDFLARLEAGQTIAYERRVQCLYE
                     AAHAHMSCTGTVYELMGVNGGRTMDTHGALQRFFRDLLAMRNHPAANLEFSASLYAQA
                     KLGVEPPPFVPTQRFVL"
     gene            complement(7205..7783)
                     /locus_tag="VGC82_07200"
     CDS             complement(7205..7783)
                     /locus_tag="VGC82_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0423093.1"
                     /translation="MANIQPNGPVAPSGLNHLVINVRNMEESHRFWTEILGFTQVGEL
                     QGRPSGAPRRGTMRFYSGDHGGGQMNHHDIALVETPSLPPASADGAPTAISHIAIALP
                     NRDAWLRQLAYLQSRGVAFERRVEHGMTHSLYIRDPNGYSVELLYELPREIWEDDIQS
                     ALNHYVSLPTDGEAALADRTDGIPVFKRSVPA"
BASE COUNT         1390 a         2664 c         2554 g         1431 t
ORIGIN      
        1 tttttgttcg cgacgaacca tctgtgcctc tgcgtcattg acataggccg ccagcaggct
       61 ttcggcgatc tccgacaggt tcggcgtgag gacgcgagct cgtcgcacaa ggtcttcgtt
      121 cagtgtcgtg ttgactggcc gcttgggcgc tgcccgatca taccccatcg aaaccgccac
      181 gatgcgcata cgatgcgcat ctgtcgcatg tcgtggcccc gaggacaaag ttgtcatgat
      241 acggccagcg ccggagctgc ccgtcggggt tctggcgctt gactgcccgt tctatgacat
      301 agtcgttgtc ggtagcacgg gccgagggat caggccgggg cacgccgata acatcgcata
      361 cctccgacag gaacagggca tagttggccc gttcctgtcc gccctcgcgg gcttcccagc
      421 gcgcgatgaa ggcgtcgggt gctgccttca tgcaatgtag ttatgatgtc cgatcattct
      481 aatttcatac taggaatggt tctgaactgg ttagtaattt gttgtttcta cgatactaca
      541 aaatatgctg ctcgatacga gcgtctgtca ctgcacccag tggatcaatt tcagctggca
      601 aaagaacgtt cggcgccggt ttgaaatccg aggaccaggc cagccttcat ccacgccggc
      661 acggtcagtt cgggtcgcgt gactctacac aacacgaaag gcggccacgg caccgcacac
      721 tcgaaaggcc gtcctctccg tggcccttcg tggccctccg cgagctccgt gtttagattt
      781 tcctgctcct ggcctggcac cgcattatca aggatggccc gaacggaaac aggtaaacct
      841 tccagccggg cggaaccgtt gccgcgcccc ctgtcaggcc cgcacagcgc atggcacaat
      901 ccgtcggatg gatgaccgca tcgacacttg gctcgcagct tgcccggacc ccgccaccga
      961 cccgctcccg ggcatgcgcg aagccggcct gctcacgccc gcgccggact acgccaccgt
     1021 cgcccgaatg aaagccgcac ttgtggaacg caccggatcg ctgggcatcg ccagcgtctg
     1081 gggcggcagg caactcgtct tccgccattt tctgtcgttc gggacggacg cccaacgtgc
     1141 cgaatggcaa ggccgctccc tcgcggtggc gatctccgaa cccaaggttg gcgcccatcc
     1201 gaaactgctg actacgcgcg ccgcagcggc cagcggcgaa taccacatcg ccggccagaa
     1261 agcgtgggtc agcaacggcc catccgccga cgcgatcatc gtcttcgcca tcactgccga
     1321 ggacgccggc cgcaagcgat acagcgcctt catcgtcccc cgcgacaccc cggggctgac
     1381 catggccgac atgcccggct tccacgcctt gcgcccctcc cgccactgtc tgctgacgct
     1441 ggactgcgtc atcccgacgt ccgccaggct cggttccccc ggttcggcct acgaacggat
     1501 ggcactgccg ttccgtgaca tcgaggacgc ggtcggcacg ttcgcaacgc tcggcgcgtt
     1561 tcgctacgcc gcgggcctgt tcgctggaca acccatggac caagcggagg atctcggggc
     1621 gatcgtggcc ctgaccgccg tctttgccgc cgccgccgag gtggtggtcg ccgcgctcga
     1681 ctctggaagt ttccgcaccg gaaacgcgac gctggtgggg ttgagggtgc tggcactgga
     1741 tatcgtcgcg cggctgcggg cgctgcctgg aaacgctctg ttcacgacaa tcctggccga
     1801 cctggatgcc gtcctgtcca tcgccagagg tgcccgcctc gcacgtcagg cgcatctcgg
     1861 ggaagccgcc cttcgtccgg tggcagcccg tcccgaatga ccgtttcgcg ttgccactgg
     1921 gccgagacct ccgatgccgt catgcaggcc taccacgacc acgaatgggg cgtgccgcag
     1981 catgacgacc gcgccctgtt cgagctattg acgctggagg gtgcgcaggc cggcctgtcc
     2041 tggcgcaccg tcctggtccg ccgcaacgcc taccgccaag cctaccatga tttcgaaatc
     2101 gagcgcgtcg ccgcgatgac ggacgaagaa ctgtcccgcc tgctgtccga ctcgccgctg
     2161 atccgcaacc ggctgaaaat ctactccgtg cgcgacaacg cccgcgcctc actcgctgcc
     2221 atcgcacaac acggcagcct cgaccactat ttgtggtcct tcgtcggcca cacctcgatc
     2281 cgcaacaatc cgaccgaccg cttccaggtg ccggcaacca caccggtatc cgatgccatg
     2341 agcaaggcgc tgaaaaaggc cggcttccgt tttgtcggtt ccacaatatg ctatgccttc
     2401 atgcaggcca cgggaatggt cgacgatcat ctgacaagct gctaccggca cccccaggag
     2461 taaccaatgc agaataccga ggaagtctgg cgcctggttg acgcccacca ggaggatttc
     2521 atccaactgt ccgaccgcgt ttgggagatg ccggagctct gctacgctga attccgttcc
     2581 tgcgccgaac acaccgcgat gctggaggcc aaaggcttcc gcgttacaac cgaagtcgcc
     2641 ggcatcccga ccgccgtcat gggcgaggcc ggcgaggacg gcccggtgat cgccatcctg
     2701 ggcgaatacg acgcattgcc gggcctgtcg caggaagccg gcgtggcgca gcagaagccc
     2761 ttgcccggtg ctggcttcgg ccacggctgc gggcacaaca tgctgggctc cgcctcgatg
     2821 ctcgccgcca ccgcggtcaa ggactatctg gaagccaacg gcataccggg acgcgtccgg
     2881 tactacggct gcccggccga ggaaggcggc ggcgccaagg gcttcatggc tcgagccggc
     2941 gcattcagcg acgtcgatat cgccatttcg tggcatccgt cgtcgttctc cggggtgaac
     3001 ccggccaact cgctggcgaa cacccgcatc gatttctcgt tccacggcag ggcctcgcac
     3061 gccgccgcct cgccgcacct gggccgcagt gcgcttgacg cggtcgaact tatgagcgtc
     3121 ggcgtgaact acatgcgcga gcacatgccg tccgacgcgc gtgttcacgc cgcgatcctg
     3181 gatgccggcg gcgtggcgcc caacgtggtg caggcgttcg cgaaggtccg ctatttggtc
     3241 cgcgccgcga cgcttccgga actgtccagc ctgatcgccc gcgtccgcaa gatcgccgac
     3301 ggcgccgcgc tgatgacgga aacccgcgtg accgcccagg ttgtcagcgc cgtgtccaac
     3361 ctgctggcga acaccccgtt ggagcgtgcc atgcaggaca acctggaacg catcggcccg
     3421 ccgccattcg acgaagccga ccgcaccgcc gcccgtgaat tccaggcgac gctgacggat
     3481 gaggatatcg aggcatccta ccgccgagcg ggcatcccgg tgcggccgga tacgccgctg
     3541 tgcgacgtga tcattccgtt ggatgccaag cccagcggcg gcgtcgggtc caccgatgtc
     3601 ggcgacgtaa gttgggtggt tccgactgtg caggcgcgtg gcgcgaccta tgcgatcggc
     3661 acgcccggac actcctggca gttgaccgcg cagggcaaga tgccggcagc gcataaaggc
     3721 ctggtgcatg tcgccaaggt catggccggc accgccctgg atgcgatccg ggacgagacc
     3781 ttgctggcga gagccaaggc ggatcaccag gcccgggtgg gaaaaacccc gtatgtgtgc
     3841 ccgctgcccg acgaactcga tccgccgatc aagatgtcgg cctgatgagt ggatgcgaat
     3901 gcatccccgg aagcgtaacc aggtgatcgc ctgatggaca cgccactgtg ccgcatgctc
     3961 ggggtcgcgt ttcccttggt ggccttcagc cactgccgcg atgtcgtcgc atcagtcagc
     4021 cggtcgggcg gtttcggcgt gttcggcgcc gccggctgca cgccggagca gcttgagacg
     4081 gagcttgcct ggatcgacgc gcatgtggac ggccacgcgt acggcgtcga tctcctggtg
     4141 ccggagaacc tgtcgatccg cacggcagcc ggcgccacgc aaggcgatct tgtggcccgc
     4201 gtgccagatg ggcatcgggc attcgtccac gacctgctgc gccgccatga cgtcgatccg
     4261 acccgcggca gttacgaccg cgaggatccc aatcgcccgc cgtcatatca gttcgagacc
     4321 ggcgaaaggc tgatggatgt cgccttccgc caccccatcc ggctgatcgc caacgcgttg
     4381 ggcgtgccgc cggcttcgat gctggaacgt gctcgccgcc acggcattcc ctgtgccgcg
     4441 ctggtcgggg cgcgtgaaca cgcgcttcgc caggtcgcgg ccggggttga catcatcgtt
     4501 gcccagggcg gcgaagccgg cggccattgc ggcgaggttt ccaccctggt gctggttccc
     4561 gaagtgctgc gagcagtgca aggcgccagg ggtcaaggta tcgtgcccgt cctcgccgcc
     4621 ggcggcatca tgaccggtcg gcagatggcc ggctgcatgg cgatgggcgc agccggcgcc
     4681 tggaccggtt cagtgtggct ggcgacgacc gaggccgaga cctccccgat cttccgcgag
     4741 aaaatgctgg ctgcccggtc ccgcgacacc gtccgctccc gtggccgcac cggcaagccg
     4801 gcgcgacaac tgcgttccgc ctggaccgac gcgtgggagg cgccggacag tcccggcgcg
     4861 ttgccgatgc cgctgcaagg cctgatcagc gagccggcca tggcccggat caaccgggca
     4921 gccgaagcgg gcaatgcgaa cgctcgccaa ctggtcagct actttgtcgg gcaaggcgtc
     4981 ggtctggtgg acactatccc gacctgccgc caggtcgtcg ccgcgttcat gaccgagttc
     5041 gcagaggcgc tggacgacat gcagcgttta tcagaagcag gaggctgaca tggtgaaacg
     5101 cctgactgac gccgaacgtt cgacactggg cacatcggtt ccgggctggg tacaggcgcc
     5161 cgaccgcgac gcgatccgcc gcgaattcaa gtttgccgat ttcaacgagg cctggggctt
     5221 catgtgccgg gtcgcgctgc tggccgaaaa acaggaccac catccggact ggtcgaacgt
     5281 ctggaacacg gttcggatcg agctatccac ccacgatgtc ggcggattat ccgacaacga
     5341 cgtccggctg gcccaggcca ttaataaaat cctcggctga tggacggcat tggctttcct
     5401 gaacgcacca gcctggtgcg caaagccgct gctcggctat gcctgcggct cggctggtcg
     5461 ccgctgcacg aggtgcggct ggcgaatggc cggcgcgcgg acatcctggc gttgcagccg
     5521 ggcggcggat ttgtctgtat cgaggtcaag tcgggtctgc gcgattacct gtcggacgaa
     5581 aaatggaccg actatcagcc gttctgcgac cagttcttct tcgcggtgga ctgtgacttc
     5641 ccgaacgaca tcttgccacc cgagaccggt ttgatcgtcg ccgcggggcg cgaagccgac
     5701 gtgctgcgcg agcctttggc ggtgcggctt tccgcgcctc ggcgtaaggc gttgctggag
     5761 cgattcgcgt ggctggctgc agcgcgcctg gcagcgctgg aagacccgat gggtgtagcg
     5821 gaactggcgc tgggattaag cgaggactga gtaccagccg tcaaaggaca aaacgctgcg
     5881 tgggaacgaa cggcggtggc tccacgccga gtttcgcctg ggcgtataag ctggcgctga
     5941 actccaggtt ggcggccgga tgattgcgca tcgccagcag gtcgcggaaa aaccgctgga
     6001 gcgcaccatg cgtgtccatg gtgcggcctc cgttgacgcc cattagctcg tacacggtgc
     6061 cagtgcagct catgtgcgca tgggccgctt cgtacaggca ctgcacgcgg cgctcgtaag
     6121 cgatggtctg gccagcttcg aggcgtgcca ggaaatcgct ccagatcgca tgcattcgcg
     6181 tcctggcggc gtccacttcc gtcaatgcgc gtgcgagggg ccgatgcagc gccggattct
     6241 gtgcaacggg aagaccgccg tacgccgaaa cccggctttg gttctgctcg ataaaggcat
     6301 ccagcgcacc cgccgccgcg ccgatcgccg gcgccgcgat ggtggtagcg aaaaccagcc
     6361 gccacggcag ccggtagtac ggccggtcat tgaccgcgaa gcccgggtcg gtgccacggt
     6421 aaacgtcaac gatccggtgt gtgcgatgtt ccggaacgaa ggcgccgcgc acgacaacat
     6481 tcttgctgcc ggttccgccc agtccggtca cactccagct ttcctggtct atggcgaaat
     6541 cccgccgagg cacgaggaaa gtgcggaatt ccgcaccacc gtccgcatcc tcgggcacca
     6601 ctgccccgag cagcgcccag ccgcaatgat ccaccccgct ggagaagtgc cagcgtccgg
     6661 acaaatggaa gccgtcgccc tcccgccgca ccgaaccggt gggggcatag gaggatgaag
     6721 cccgagcgtc cgggtcggtc gcccaaacgt cgcgttgggc ctcgttcgcg aatagcgcga
     6781 tctgccacgg atgaatgccg acgacgcttg cgacccagcc ggtggacccg caggcggagg
     6841 cgatccggat catgccctcg taaaaggtcg cgaggtcgag ttccaggccg ccccattggc
     6901 ggggctgcgc ggcacggaat acgccggcat cggtcaggcg ttgaacgatg tcttccggca
     6961 cccgtccaag ccgttcggtt tcgcccgcac gggcacgaat tgtcggcagc aaatcgtcta
     7021 tgccagcaag gaaactctgc cttgggtctt cgatggcatc ggattgttta accggagcca
     7081 ggtccagcat gagcatctcc cgtggcgttg tttccgcttc ttcggtggcg gagtattcgt
     7141 ttcatcggcc aacaacctga aacgctgtcc gggctggggc ggcgtctcag gctggggcgt
     7201 cgtctcaggc tgggacagag cgcttaaaca ccggaatgcc gtcggtcctg tcggccaacg
     7261 cagcctcgcc atccgtcggc aacgacacgt aatggttcag cgctgactgg atgtcgtctt
     7321 cccagatctc tcgcggcagc tcatagagca gttcaacgct gtatccgttg ggatcacgga
     7381 tgtacaggct atgcgtcatc ccgtgctcca cccgccgttc aaacgccacg ccgcggcttt
     7441 gcaggtaagc gagctggcgc agccacgcgt cacggtttgg cagggcgatc gcgatgtggc
     7501 tgatcgccgt cggggcgcca tccgcggagg ccggtggcag gcttggagtc tctaccagtg
     7561 cgatgtcgtg gtggttcatt tggccgccgc cgtgatcgcc gctgtagaag cgcatggtcc
     7621 cgcgccgagg ggcaccactg ggccgacctt gaagctctcc aacctgggta aatcccagga
     7681 tctccgtcca gaaccggtgc gactcttcca tgttccggac gttgatgacc aaatgattga
     7741 ggcctgacgg cgccacgggt ccgtttggtt gaatattggc cacggcttcg ctcctctgca
     7801 ttccgtatga tttatttgca tggtattgcg cagaacaggc ggacgttctc ctacgaaagc
     7861 ccataggtca agccctgcgt tggatctctg gcgcatggac cggcggcccc tgccgaagca
     7921 cggcggcgcc ggcgccaaac cgtggctacc ttggatcagc cagtccgttg tgccatgatt
     7981 ccttcaatgc cgcgaagcaa ccacggccac cgatggagga gaacggaaat gtcggacaa
//
LOCUS       DASWQN010000437         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1889467, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000437 DASWQN010000000
VERSION     DASWQN010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889467"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VGC82_07205"
     CDS             complement(<1..1052)
                     /locus_tag="VGC82_07205"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEY0423094.1"
                     /translation="MNKTTQTVESAATGAFHIEDVYPAVDGGRFPVKRIVGERVDVWA
                     DIYRDGHDLVSAALVWRLERDREWRREPMTHRGNDRWSGFFVPDHPGRYVYAIEAWTD
                     EFATWRHGFELKQQAGADLTLDAIEGAGMLTKAQAGGHAAATVILKQCEDFLQTGDPA
                     PLLTAELKDAMAESQLRPDLTRSELFPFVADRPRARTGAWYEMVPRSQGNTPGQHGTF
                     KDCIARLPDVAAMGFDVLYFTPIHPIGRINRKGRNNAVTAAEGDPGSPYAIGAAEGGH
                     DAVHPELGTLEDFRELVAACQGFGMEVALDVAVQCSPDHPWLKQHPQWFKRRPDGSMR
                     YAENPPKKYEDIVNPD"
     gene            1253..>2871
                     /gene="malQ"
                     /locus_tag="VGC82_07210"
     CDS             1253..>2871
                     /gene="malQ"
                     /locus_tag="VGC82_07210"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY0423095.1"
                     /translation="MDLFTKAKELGIQTEFIDGQGYPHVTDEAALKVIIDALPVRAPA
                     RFLAGPVVVRSGQPARTELTQAAALPVRWKVVAGLKVIAEGETSYRAIIWPPDLPVGS
                     YRLHLTDGEFSEHAPLIVAPARAFGGDFDRCWLLAVQLYGVRSARNWGIGDFTDLQAL
                     LELARELGADGIGLNPLHALFDDRPSDCSPYSPNSRLFLNPLYIDVENLPEFKPGAVA
                     GSGDVLARLRAGDIVDYAAVAELKWRALRLAFEQFRAKPDTTRNEDFTRFRAECGPLL
                     SRFATFEVLRHKFSKPWWEWPEQWQRPDDAETARLREGDDAAEIEFIEFVQWAADRQL
                     AACRDQATRLGMKVGLYLDVAVGVQTDGFDAWNEQAAISRHLGVGAPPDALNTAGQDW
                     GLAGFNAAGLEIQSFEPYRQMLRASMRHAGAIRLDHVLGLKRLYLVPHGFAPDNGVYV
                     PMPFEALLAVTAQESVAHRCVVIGEDLGTVPEGFREQMADWGIWSYQVMMFERDDQGR
                     FRDVDYYTANSLVTFNTHDLSTYAGWRCLSDL"
BASE COUNT          506 a          897 c          925 g          543 t
ORIGIN      
        1 aagtcggggt tgacgatgtc ctcgtacttc ttgggcgggt tctccgcgta gcgcatcgag
       61 ccgtcgggcc ggcgcttgaa ccattgcgga tgctgcttga gccaggggtg gtcgggcgaa
      121 cattgcacgg cgacatcgag cgcgacttcc ataccgaagc cctggcaagc cgcgaccaat
      181 tcgcggaaat cttccagcgt gccgagttct ggatgaacgg cgtcgtgacc gccttcggcc
      241 gccccgatcg cataggggct cccgggatcg ccttcagccg cggtcacggc gttgttgcgg
      301 cccttgcggt tgatgcggcc gatcggatgg atcggcgtaa aatagaggac atcgaagccc
      361 atcgcggcga catccggcaa ccgcgcgatg caatccttga aagtgccgtg ctggcccggc
      421 gtattgccct ggctgcgtgg caccatctcg taccaggctc cggtgcgcgc ccgcgggcgg
      481 tcggcgacga acggaaacaa ttccgaacgg gtcaggtcgg gacgcaactg actctcggcc
      541 atggcgtctt tcagctcggc cgtcagcagg ggcgcgggat cgccggtctg aaggaaatcc
      601 tcgcactgct tcaagatgac cgtggcggcg gcgtgtccgc cggcctgggc cttggtgagc
      661 atgccggcgc cttcgatggc atcgagggtc aggtcggcac cggcttgctg cttgagctcg
      721 aaaccgtgcc gccaagtcgc aaattcgtcg gtccatgcct cgatcgcgta aacgtagcgt
      781 ccgggatgat ccggcacgaa gaatccgctc cagcggtcgt tgccccgatg ggtcatcggc
      841 tcgcggcgcc actcgcggtc ccgctccaga cgccacacca gtgccgcact gacgaggtca
      901 tgtccgtcgc gatagatgtc tgcccagaca tcgacgcgct cgccaacgat ccgctttacc
      961 ggaaagcggc cgccatccac cgcgggataa acgtcttcga tatggaaagc gccggtcgcg
     1021 gcgctctcga cagtttgagt tgttttgttc acggtgatgc cattgacacg gaaaaaacgt
     1081 cccgtttcga ttgcaggaaa tgagacgctc cagagctgta tagaaagccg atggccggac
     1141 attggttccc ggggaacggc tgagacgcta cagagttaaa caccgctatc ctcttacctc
     1201 atcaacgaaa tcttcgcgac gcccttgctg ttgggtgcaa accgcgtgcc gaatggacct
     1261 tttcaccaaa gccaaagaac tgggtattca gaccgaattt atcgacggtc aaggttaccc
     1321 gcatgtgacg gacgaggccg cgctgaaggt catcatcgac gccttgccgg tccgcgcacc
     1381 ggcccggttc ctggccgggc cggtcgtggt gaggtccggc caaccggccc gaaccgagct
     1441 gacccaagct gccgcactcc cggtgcgctg gaaagtcgtt gccggcctta aggttatcgc
     1501 ggagggcgaa acctcgtacc gagccatcat ttggccgccg gatctgccgg ttggctccta
     1561 ccggctgcat ctcaccgacg gcgaattcag cgaacatgcc cccttgatcg tggcgccggc
     1621 aagggccttc ggcggtgatt tcgaccgctg ttggctgttg gccgtccagc tctacggggt
     1681 gcggtcggcc cgcaactggg gaatcggaga ttttaccgac cttcaggcac tgcttgaatt
     1741 ggctcgcgag ctgggggcgg acggcatcgg tcttaacccg ctgcacgcgc tgttcgacga
     1801 ccggccttcg gattgcagcc cctattcccc gaacagccgg ctgtttctca atccgctcta
     1861 tatcgacgtc gaaaacctgc cggaattcaa acccggcgct gttgccggga gcggcgacgt
     1921 gctcgccagg ctgcgagcgg gggacatcgt cgattacgcc gcggtggccg agctgaaatg
     1981 gcgcgcgctg cggctggcgt tcgaacaatt cagggcgaag ccagacacca cacggaacga
     2041 ggatttcacg cgattccgcg ccgagtgcgg acccctgcta tcgcgcttcg ccaccttcga
     2101 ggtgttgcgg cacaagttca gcaagccgtg gtgggaatgg ccggagcagt ggcagcggcc
     2161 tgatgacgcc gagacggcca ggctgcggga aggggacgat gccgccgaga ttgaatttat
     2221 cgagttcgtg caatgggccg ccgatcgcca gcttgccgcc tgccgggatc aagcgacccg
     2281 cctcggcatg aaggtcggcc tctatctcga cgtcgcggtc ggggtgcaga ccgacggttt
     2341 cgatgcctgg aacgagcagg ccgcgatatc ccgtcatctg ggggtcggcg cgccgccaga
     2401 cgcgctgaac accgccggcc aggactgggg ccttgccggg ttcaatgccg ccggtctgga
     2461 aatccagtcg ttcgagcctt accgccagat gctgcgagcc tccatgcgcc acgccggcgc
     2521 gatccggctc gatcatgtgc tgggcctgaa gcggctctat ctggtgccgc acggctttgc
     2581 tcccgacaac ggcgtctacg taccgatgcc gttcgaggcg ttgctggcgg tcaccgcgca
     2641 ggaaagcgtg gcgcatcgtt gcgtggtgat cggcgaagac ctcggcaccg tgccggaagg
     2701 ctttcgcgag cagatggcgg attggggcat ctggtcgtac caggtgatga tgtttgagcg
     2761 cgacgatcag ggcaggtttc gcgacgtcga ctattataca gccaattcac tggtcacctt
     2821 caatacccac gatctctcga cctatgccgg ctggcgctgc ctcagcgatc t
//
LOCUS       DASWQN010000438         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_438050, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000438 DASWQN010000000
VERSION     DASWQN010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438050"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VGC82_07215"
     CDS             <1..1124
                     /locus_tag="VGC82_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY0423096.1"
                     /translation="LTRGRANTLRLALTGQLGADAMTSDAMADAMALCVSCKACKREC
                     PTGVDMAKMKIEVTAARAERFGLRWRETLVGELPRYAPFVSRITGLANLRNNLPPLRW
                     LTERALGFSASRRLPRWRRDKFLDREAAAAAPVSPRGDVYLLADTFNRYFEPENLRAA
                     LTVLAAAGYRAVIPKTSGRPLCCGRTWLAVGMVDKAREEAERTMRHMPDNLPIVGLEP
                     SCLMTLRDEFKSLLPGAAADRFAERSLLLSEFIARERPSLKLNALPGTARVHGHCHQK
                     ALGAFPDALAMLRLIPRLSAQPIASSCCGMAGTFGYQAETEATSRAMAEAGLLPAVRN
                     AAEHDYIVADGTSCRHQIRDLSGREAIHSVRLMERALQG"
     gene            1235..>1517
                     /locus_tag="VGC82_07220"
     CDS             1235..>1517
                     /locus_tag="VGC82_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423097.1"
                     /translation="MTETIGSGPFTYKAEDGWGKLPDGYEFGDVAAVGVDRWDNVYAF
                     HRGEHPMVVFDKDGNFLRSWGDGIFKRAHGVHMAPDDTIYCTDDGDHTVR"
BASE COUNT          270 a          485 c          504 g          258 t
ORIGIN      
        1 acttgactcg aggacgcgcg aacaccctgc gcctggcgct gaccggccag ttaggggccg
       61 acgccatgac ctccgatgca atggccgacg caatggcgtt gtgcgtgtcc tgcaaggcct
      121 gcaaacgcga atgtcccact ggcgtcgata tggcgaagat gaagatcgag gtcactgccg
      181 cacgcgccga acggttcgga ctgcggtggc gggaaaccct ggttggcgag ttgccgcggt
      241 acgcgccgtt cgtctccagg atcacagggt tggcaaacct gcgcaacaat ctgccgccgt
      301 tgcgctggct gaccgaacgc gctcttgggt tctccgccag ccggcgcctt cccagatggc
      361 ggcgcgacaa attcctcgac cgtgaagcgg ccgccgccgc cccggtctcg ccgcgcggcg
      421 atgtctatct gctggcggac accttcaacc gctacttcga accggagaac ctgcgtgcgg
      481 cgctgacggt tttggccgcc gccggctacc gcgcggtgat cccgaagacc tccggccgcc
      541 cattgtgctg tgggcgcacc tggctggccg ttgggatggt ggataaggcg cgcgaggaag
      601 cggaacgcac catgcgccac atgcctgaca acctgccgat cgtcggactg gaaccatcct
      661 gcctgatgac gttgcgcgac gagtttaaat cgctgctccc cggtgccgcc gcggatcgtt
      721 tcgcagaacg gtcgttgttg ctgagcgagt tcatcgccag ggagaggccc agcctgaaac
      781 tgaacgcgct gcccggaacc gcgcgcgtgc acggccactg tcatcagaag gcattgggtg
      841 catttcccga cgccttggcg atgttgcggc tgatcccccg gctctcggcg caaccgatcg
      901 cgtcgtcatg ttgcggcatg gccggcacct tcggctatca ggcggaaacc gaagcgacct
      961 cgcgggcgat ggccgaggcc ggcctgttgc cagcggtgcg aaacgccgcc gaacacgact
     1021 acatcgtcgc cgacggcacg tcttgccggc accagatccg cgacctgtcg ggacgtgagg
     1081 caatacattc cgtgcgactt atggagcgag ccttgcaggg gtaggccgcc actggatggc
     1141 ctatccggct gtcgtgcgct aggctggcgg gaacgtcgcg ggaggcatga gacggttccg
     1201 tccgcgtcca aggacagtcg aggggaaacc agatatgacc gaaacaatcg gcagcggacc
     1261 attcacctac aaagcggaag acggctgggg caagctgccg gacggttatg aattcggcga
     1321 cgtcgccgcg gtcggggtgg accgctggga caatgtgtat gcgttccatc gcggcgagca
     1381 tccgatggtc gtcttcgaca aggacggcaa ctttctgcgc tcctggggtg acggcatctt
     1441 caagcgggcg catggcgtgc acatggcgcc ggacgacacg atctactgca ccgacgacgg
     1501 cgaccacacc gtccgca
//
LOCUS       DASWQN010000439         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_570055, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000439 DASWQN010000000
VERSION     DASWQN010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570055"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..775
                     /locus_tag="VGC82_07225"
     CDS             14..775
                     /locus_tag="VGC82_07225"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0423098.1"
                     /translation="MGLASYIVAILTTRYGMGHGATAIISIVGTTASAALFGLIALRA
                     TGLSFLMITLAISQVLWGLAYRMTSVTNGDNGITGITRPMPFGVSLESASSFYWFTLI
                     VTAASFAAMATFVLSTFGSSLRGVRDQPRRMAALGYNPWMIRWITFIYSGFWAGVAGL
                     LYVYYHKYIHPTSLSTTNSAEGLLGVIAGGSGTLGGPVVGAALVSLLKNYASGYIERW
                     NMLLGIVFLLIVLLMPTGIVPGIRKLVGRIRGGQK"
     gene            772..1521
                     /locus_tag="VGC82_07230"
     CDS             772..1521
                     /locus_tag="VGC82_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0423099.1"
                     /translation="MTTPEYALEVSNLCKSFGGLAVTQNVTLKIRPGERRLIIGPNGA
                     GKTTLFNQITGDLMPSSGKVQLFGKDITRLRPDQRAHFGLSRTYQIITLFAQDTLEHN
                     VALGLMGLRSARWHTIRPMSFYKDIAHESRRILGLVGLENRASLPVSDIAYGEKRRVE
                     LTMALAQKPRLLLLDEPLAGLSSSERTTVKSLIASIPRETAVVMIEHDMDTALDLAET
                     VTLLNYGKVIVDGERDAVIADEKTREVYLGS"
     gene            1511..>1722
                     /locus_tag="VGC82_07235"
     CDS             1511..>1722
                     /locus_tag="VGC82_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0423100.1"
                     /translation="MAADALTLTNVHAFYGDSHVLHGISFTLQAGQVLALLGRNGAGK
                     TTCISTTVGFLSPREGDILLFGKSISG"
BASE COUNT          327 a          520 c          497 g          378 t
ORIGIN      
        1 ccacgcctca tatatggggc tggcatccta catagtggcg atcctgacca cacgatacgg
       61 catgggacac ggcgcaacgg cgatcatatc aatcgtcggc acaaccgcat cggccgcttt
      121 gttcggcctg attgccctgc gtgcaacggg gctgagtttt ctcatgatca cgctggcgat
      181 atcgcaagtt ctctggggtc ttgcctatcg gatgacatcc gtcaccaatg gcgacaacgg
      241 catcaccggc atcacccgcc cgatgccgtt cggcgtctca ctggaaagcg catcttcgtt
      301 ctactggttc acgctgattg tgaccgcggc gagcttcgct gccatggcca ctttcgtgct
      361 ttccaccttc ggctcttcgc tcaggggcgt tcgtgaccag ccccgtcgga tggctgcgct
      421 gggctacaat ccgtggatga tccgctggat cacgtttatc tactccggct tctgggctgg
      481 agttgccgga ctgttgtacg tttactacca taagtacatt catccgacat cgctctcaac
      541 aacgaactct gcggaaggac ttcttggcgt tatcgccggt ggatccggca cgcttggcgg
      601 cccggtggtt ggcgccgccc ttgtgagcct gttgaaaaac tatgcgtccg gctacatcga
      661 acgctggaat atgctgcttg gcattgtatt tctgctgatc gttctgctga tgccaaccgg
      721 catcgtgccc ggcattcgca agcttgtcgg gcgcatcagg ggcggccaga aatgaccact
      781 ccggaatacg cgcttgaggt cagcaatctc tgcaaatcgt tcggcggact tgcggttacg
      841 caaaacgtca cgctgaaaat acgccccggc gagcgacgtc tcattatcgg gccaaacggc
      901 gccggcaaga caacgctgtt caaccagatc actggcgacc tgatgccaag ctccggcaag
      961 gtacagttgt tcggcaagga catcacccga ttgcggccgg atcagcgcgc gcatttcggg
     1021 ctgtcgcgca cgtatcagat catcacgctg ttcgcgcagg atacgctgga acataatgtg
     1081 gcgctgggat tgatgggtct acgatcggct cgctggcaca ccatccgacc catgtccttc
     1141 tataaggata tcgcgcatga gtcgcgccgg attctcggtt tggtggggct tgagaaccgg
     1201 gcatcgcttc ccgtctcgga tatcgcctat ggcgagaaac ggcgggtcga gctgaccatg
     1261 gccttggcgc aaaaaccacg tttgcttttg ctcgatgaac cgctcgcagg cctgtcgagc
     1321 tccgagcgca ccaccgttaa atcgctgatc gcctccattc ccagggaaac cgcggtggtg
     1381 atgatcgagc atgacatgga cacggccctt gacctggccg agaccgtcac gctgctgaac
     1441 tacggcaagg tcattgttga cggcgagcgc gacgcggtca tcgcagacga gaagacgcgg
     1501 gaggtgtatc ttggcagctg acgcccttac cctcacgaat gttcatgcgt tctatggcga
     1561 cagccatgtc ctgcacggga tcagtttcac cctccaggcc ggacaggtcc tcgcgctgct
     1621 gggccgcaac ggcgcgggca agacgacgtg catttcgacg acggtcggct ttctctcgcc
     1681 gcgcgaaggc gacatcctcc tgtttggaaa atcgatctcc gg
//
LOCUS       DASWQN010000440         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1230007, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000440 DASWQN010000000
VERSION     DASWQN010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230007"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VGC82_07240"
     CDS             complement(<1..227)
                     /locus_tag="VGC82_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423101.1"
                     /translation="MYKANISKINNLLVCSDRNISEYVQLNTGDAALCRQAAEDGDRL
                     KKRTGSFSRSVCYWRPNADRLKEVLPNSLLG"
     gene            908..1513
                     /locus_tag="VGC82_07245"
     CDS             908..1513
                     /locus_tag="VGC82_07245"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY0423102.1"
                     /translation="MTDRSVILVGMKATGTRDHLISTGLKILHARGFHASGVQDITQA
                     AKVPKGSFYTHFDSKERFGAEVLGRYWEHRADSAIAILSDEARTPLDRLKAYFASKTG
                     VSGPFDKGCMIGNFSAEMAGDSRLMRDRLAAVLAAWTQMLATCIREAQAAGQLRDSLD
                     PDSVAAFLIDAFEGAVLRTKVDRDASALLRFESIVFSIILT"
     gene            1525..2922
                     /locus_tag="VGC82_07250"
     CDS             1525..2922
                     /locus_tag="VGC82_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY0423103.1"
                     /translation="MSATVLSDRSVNISPPSMDGRTRALLDAPLLALLLRLAAPNILL
                     MLAQSATGLIETYFVGKLGTEALAGVSIVFPGVMLMQMISAGAMGGGISSSIARALGS
                     GRREDASDLVWHALVINGVLGVICTAVVLTFGTSLYRALGGSDGSLRTALQYSNIIYA
                     GAILLWLMNALSSAIRGTGNMLLPAIVICGGALLLIPVSPCLIFGWGPFPRLGVAGGA
                     VALIGYYVFGIVLQGLYLFAGRSVIRPRLSGVRLQTRLFGAILKVGGIASLSSIQTNL
                     AIMITTGMVGAFGPAALAGFGTGSRLEYLMVPIAFGVGGPLVAIVGTNIGAGQRQRAL
                     RAAWIGAAIGFGITEAIGIAAALFPVAWLSLFDTDPAMLATGTLYLRCVGPFYGFFGL
                     GLVLYFASQGAGRMAWPVTAQFMRLVVAGGGSGIAVWLGGGLMSVFMLIGIALAVFGL
                     GVAASIAGGSWSARH"
BASE COUNT          490 a          842 c          937 g          657 t
ORIGIN      
        1 aatccaagta gactatttgg taatacttcc ttcaatcgat cagcgttggg tcgccagtaa
       61 caaaccgagc ggctaaacga gcccgtgcgt tttttcagtc gatcgccgtc ttcggctgcc
      121 tgtcggcaaa gcgcggcatc cccggtgttc agctgaacat attcggaaat attacggtcg
      181 gagcagacaa gaaggttatt gattttcgat atgtttgctt tatacacacc gtgtattagg
      241 ccgcgggccg gttgaactgg atcgctctat agcgaccgga ccatagaaaa taaacattta
      301 ctaatgtgga ctccgcaaat aatgcggata tttatcgaaa gcgcatggcc tgcggctgcc
      361 aaacagcaaa tcgtgtagcg gtccaggtcg gtccttcttg ttggagtatg ccgggcgccg
      421 tgatccgttt gcgggatgcc caatgggcga aattacccga acgccctctg tgaagaaggt
      481 gcgccactag cgtgatgcgg gttgcgtttc attcacggtt attgctcgaa gtgccgggtg
      541 acggtttgat ccggctggcc agatcttaaa cggccggcta cgacattgcc tggtgcgcaa
      601 tggcatatgg cctgccgtct ttgcctcgtt ggctagagca ttcccaggct gattgtacat
      661 tcgaaaatgt cctatcctct tgctcgatcg cgtgattcac acgcaacgcc cgaacagcgc
      721 gtccgtcgga cgacgcccga acagcgcgtc cgtcggacga cgggcgctcc gcccagcgtg
      781 atcacgctcc aggagcaagc cgggtcttga aggtggtggt gttggtccgc cgtttcgctt
      841 cgcggcgctc tggcggccaa cacaggaaag cggcaagtgg tcaggccagc ttcggaagct
      901 cttgtaaatg accgaccggt cggtcatatt agttggcatg aaagcgaccg gcacacgcga
      961 ccacctgatt tccaccggct tgaagatcct gcacgctcgc ggctttcatg ccagcggcgt
     1021 gcaggacatc acccaggcgg cgaaggttcc gaaaggctcg ttctacacgc acttcgacag
     1081 caaggaacgc ttcggcgccg aggtgctggg tcgctactgg gaacatcgtg cggactcggc
     1141 catcgccatc ctcagtgatg aagcgcgcac tccgctggat cggctgaagg cttacttcgc
     1201 ctcgaaaacc ggtgtctccg ggccgttcga caaaggctgc atgattggca atttctccgc
     1261 ggaaatggct ggggacagcc ggctgatgcg ggatcgtctc gccgccgtgc tggcggcttg
     1321 gacgcagatg ttggcgacct gcatccgcga agcccaggcg gcaggacaac tgcgcgacag
     1381 cctggatccc gacagcgtcg ccgccttcct gatcgatgcc ttcgagggcg cggtcctgcg
     1441 caccaaggtg gatcgcgacg ccagcgccct gctgcgcttc gaaagcatcg tcttctccat
     1501 tatcctgacc tgaggcaagc atccatgagc gccacggttc tgtccgaccg gtcggttaac
     1561 atctcgcctc catcgatgga tggacgcacc cgagcgctgc tcgatgcgcc gctgctggcg
     1621 ctgttgctaa ggctggcggc gccaaacatc ctgcttatgc tggcgcaatc ggccaccgga
     1681 ctgattgaga cttattttgt tggcaagctg ggcaccgagg cactggccgg tgtgtcgatc
     1741 gtatttcccg gcgtgatgtt gatgcagatg atttccgccg gcgccatggg tggtggcatt
     1801 tcctcctcca tcgcccgggc actgggaagc ggccgacgag aagacgcgtc ggacctggtt
     1861 tggcatgccc tggtcatcaa cggcgtgctg ggcgtgatct gtaccgccgt cgtgctgacg
     1921 tttggaacct cgttataccg ggcgctcggt ggttccgacg gatcgttacg taccgcgttg
     1981 cagtattcca acatcatata cgctggcgcc attctccttt ggctgatgaa tgcactttcc
     2041 agcgcgatcc gcggcaccgg caacatgctg ctgccggcta tcgtgatctg cggcggcgcg
     2101 ctgctgttga tcccggtgtc gccctgcctg atctttgggt gggggccgtt cccgcgcctg
     2161 ggcgttgccg gcggggcggt ggccctgatt ggctactacg tcttcggaat cgtgctgcaa
     2221 ggcctctacc tgttcgccgg ccgctcggtg atccggccga gactttcggg tgtgcgtctg
     2281 cagacgcgtt tgttcggggc tatcctgaag gtgggcggca ttgcctcgct cagttcgatc
     2341 cagaccaacc tggcgatcat gatcacgacc ggaatggtcg gtgcgttcgg accggcggca
     2401 ctggcgggtt tcggcaccgg atcccggctg gaatacctga tggttcccat cgcgttcggt
     2461 gtcggcggac cgctggtcgc gatcgtcggg actaatatcg gagcgggaca acggcaacgg
     2521 gcgttgcggg cagcctggat cggcgcggcg atcggctttg gcatcaccga agcgatcggc
     2581 atcgcggcgg ctctgtttcc cgtcgcatgg ctgtcgttgt tcgacaccga tccggcgatg
     2641 ctggccaccg ggacgctgta tttgcgttgc gtcgggccgt tctatgggtt cttcggcctg
     2701 ggattggtgc tgtattttgc gtcccagggc gctggtcgga tggcttggcc ggtgaccgca
     2761 caattcatgc gattggtcgt ggccggcggc ggaagtggca tcgcggtgtg gttgggcggc
     2821 gggttgatgt cggtgttcat gttgatcgga atcgcactgg cagtgttcgg cctgggcgtg
     2881 gcggcatcga ttgccggcgg ctcctggtcg gcgcgacatt agcgtg
//
LOCUS       DASWQN010000441         3816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1757926, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000441 DASWQN010000000
VERSION     DASWQN010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757926"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VGC82_07255"
     CDS             <1..1206
                     /locus_tag="VGC82_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0423104.1"
                     /translation="NSTLNQFSPGLGNSASTAANGVLNQLSQPVDLFQYGLSSSWELD
                     LFGRVRRSREQAKASTEAQMEATNDALVMLEGQVAQAYVQLRGAQALTATQQENVRTA
                     QEALDLTLKRQRQGLTTALDVEQARTQLANFQFLLPGYEKQSQQAMNRLSVLTGEPPG
                     TLDRMLGPSAPLPKVPAVVGIGVPSTLARRRPDIRQAEARLHAATANVGVAVANFYPD
                     ISLTGSFGLRATDASFLTNWASNFYSFGPTISLPIFQGGRLTAGLRMARAQEAEATLA
                     YRGTVLNALREVEDALVAYRTDQAARDRLVEAVRSGDLALYLARDRYTHGLADFIQVL
                     DAERTLVAARQQLVQADMMLTNDVVALYDALGGGWQEDQVHSQAPVITKTSPITPAAA
                     DSLAGEISR"
     gene            1394..2335
                     /locus_tag="VGC82_07260"
     CDS             1394..2335
                     /locus_tag="VGC82_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0423105.1"
                     /translation="MWEPNLRYPDPNVQVLDPAFGALRLPLASVEQLATGCRWSEGPV
                     YFGDGRYVLWSDIPNDRILKWEEETGAVSVFRKTSWNANGNTRDRQGRLVTCEHRGRR
                     VTRTEYDGSITVLMDSHDGKRLNSPNDVVVKSDGSIWFTDPSFGISGWYEGEKAESEI
                     RAAVWRIDGHTGQPTMVADDVEGPNGLAFSPDESILYVVASRAAPNRLIRAFDVVDGG
                     KKLAKNRIFIDAEAGTPDGFRVDIEGNLWCGWGMGAADLDGVRVFDRNARPIGHIALP
                     ERAANLCFGGRFRNRLFMAASHGLYALYVNTQGAPGG"
     gene            complement(2395..3015)
                     /locus_tag="VGC82_07265"
     CDS             complement(2395..3015)
                     /locus_tag="VGC82_07265"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY0423106.1"
                     /translation="MWLWIDAVVDLVAQYPAYALAVAFIAAIVEAVAVLGVLIPGTPI
                     LMAVAGAAAMAGQSMMPFLLLSIIGAVIGDFMSFWVGRRFSVRLGSIWPFSRRPELMV
                     NAVRFFDRYGVYSVALCRFVPVLRSTVPLVAGMAGMRQRHFVMANVASAFVWAPVHIY
                     PAQLAGLSLEQLRDGDWQSGVLWGAVLLAGCAAAWAIHRMVAARTR"
     gene            3495..>3816
                     /locus_tag="VGC82_07270"
     CDS             3495..>3816
                     /locus_tag="VGC82_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423107.1"
                     /translation="MRLRGAIFATSLLSTPVVAMAQPISGLYLGAGAGLHAPQNPRMT
                     ANSPAFGTGRLELNEDYGFNSNLAVGYGLGNGFRFEIGGDFMRSNLRSVQGLQPTRAD
                     GTIRT"
BASE COUNT          752 a         1223 c         1212 g          629 t
ORIGIN      
        1 aactcgacgt tgaaccagtt cagtccgggg ctcggcaatt cggccagcac cgcggccaac
       61 ggagtgctga accagctatc gcaacccgtt gacctgtttc agtatggcct cagttcgtca
      121 tgggaactgg atctgttcgg ccgggttcgc cggtcccgcg agcaggcgaa ggccagtacg
      181 gaagcccaaa tggaggcaac gaacgatgcg ctggtcatgc tggagggtca ggtcgcccag
      241 gcctacgtgc agcttcgcgg tgcccaggcg ctgaccgcca cgcagcagga aaacgtgcga
      301 acggcgcaag aggcgctcga tctgacactg aaacgtcagc gccagggtct gacgacggcg
      361 ctcgacgtcg agcaggcccg tacgcagttg gcgaacttcc agttcctgtt gccgggctat
      421 gaaaaacaat cgcaacaggc gatgaaccgc ctgagcgtcc tgaccggcga accgcccggc
      481 actttggaca ggatgctggg tccgtcggcg ccattgccga aggttccagc ggtggtcggc
      541 atcggcgttc cgtccacatt ggcccggcga cggcccgata tccggcaggc ggaggcaagg
      601 ttgcatgcag cgaccgcaaa tgtcggcgtg gcggtggcga acttctatcc cgatatctcg
      661 ctgaccggga gcttcggact ccgagcgacc gatgcaagct tcctgaccaa ctgggccagc
      721 aatttctatt cctttggtcc caccatttcg ctgcccatct tccagggcgg acgcctgacc
      781 gccggattgc gcatggctcg cgcacaggag gcggaggcga cgctggccta ccgcggcacg
      841 gttctgaatg cactgcggga ggtagaggac gcgcttgtcg cctaccgcac cgaccaagcg
      901 gctcgggacc ggctggtgga ggccgtgcgg tcgggggacc tggcgcttta ccttgcccgc
      961 gaccgctata cgcacggcct ggcggacttc atccaggtgc tggacgccga acgcaccttg
     1021 gtcgctgcgc ggcagcagtt ggtgcaggcc gacatgatgc tgacgaacga cgtcgtggcc
     1081 ctgtacgacg cgcttggagg tggctggcaa gaagatcagg tgcatagcca ggcgcctgtc
     1141 atcaccaaga cttcaccgat cacgccggcc gcggccgaca gcctggcggg cgagatatcc
     1201 cgataacacc gtgatctttc ggtccccagc aaccaactgg cccaccgctc tcgacgagct
     1261 tgatccgacg gttcccgtca gcgtgaagct ggtgtagctc tttgcctgat cgcatgacct
     1321 acacgctgca gacgttccgc ccggcgcgat catactctag gctacgcctt taacgaagct
     1381 gggggaatcg accatgtggg aaccgaacct gcgttatcct gatccgaacg tgcaggtgct
     1441 ggaccctgcg ttcggcgcat tgcgcctgcc gcttgcgtcc gtcgagcagt tggcgaccgg
     1501 ctgccgctgg tccgagggcc cggtctattt cggcgacggc cggtatgtgc tctggagcga
     1561 cattcccaac gaccgcatcc tgaaatggga ggaagaaacc ggcgccgtca gcgtgttccg
     1621 aaaaacctcc tggaacgcca acggcaacac ccgcgaccgg caggggcgcc tggtgacatg
     1681 cgagcatcgt ggccgccggg tcacccggac ggaatatgac ggcagcatca ccgtcctgat
     1741 ggacagccac gacggcaagc ggctgaactc gcccaacgac gtggtggtga agtccgacgg
     1801 ctcgatctgg ttcaccgatc cctcgtttgg catcagcggc tggtatgagg gcgagaaggc
     1861 tgaatcggag atccgggccg ccgtctggcg catcgacggc cacaccgggc agccaacgat
     1921 ggtggccgac gatgtcgagg gtccgaacgg gctcgcgttc tcgcccgatg aatcgatcct
     1981 gtacgtcgtg gcctcgcgcg ccgcgccgaa ccggttgatc cgggcgttcg atgtcgtgga
     2041 cggcgggaag aagctggcca agaatcgcat ctttatcgat gccgaagccg gcacgccgga
     2101 tggcttccgc gtcgatatcg aaggcaattt gtggtgcggg tggggcatgg gcgctgccga
     2161 cctggacggc gtacgggtat tcgacaggaa cgccaggccg atcggccaca tcgcgttacc
     2221 ggagcgtgcc gccaacctgt gcttcggcgg acgtttccgc aaccggctgt tcatggcggc
     2281 gagccacggt ctttatgccc tgtatgtaaa cacccagggg gcaccaggag gttagagggg
     2341 acagcctgaa gcagggggtc gcctcgggtt gcagcgatct ttaccggcgg ccgatcaccg
     2401 tgtgcgcgcg gcgaccatcc ggtgaattgc ccaggcagca gcgcacccgg ccaacaagac
     2461 cgcgccccaa agaacaccgg actgccagtc gccgtccctg agctgttcaa gcgacagacc
     2521 tgccaactga gcgggataga tatgcacggg cgcccacacg aaggcagacg cgacgttggc
     2581 catcacgaag tgcctttgtc tcatgcccgc catcccggcg accagcggga cagtcgagcg
     2641 cagcaccgga acgaaccggc acagcgcgac gctgtaaacg ccatagcgat cgaaaaagcg
     2701 caccgcgttt accatcagtt ccggacggcg ggagaacggc catatgctgc ccaatcgcac
     2761 gctgaagcgc cgtccaaccc agaacgacat aaaatcgccg atgaccgcac cgatgatcga
     2821 tagcagcagg aacggcatca tcgattgccc ggccatggcc gcggcaccgg ccaccgccat
     2881 gaggattggg gtgccgggga tcaggacgcc aagaaccgcc accgcctcga cgatagcggc
     2941 aatgaatgcc acagccagcg cataggctgg atactgcgcg acaaggtcca cgacggcgtc
     3001 gatccatagc cacattggac gaaacgacgc tcctgtatca aatggtaacg gccagtatgg
     3061 cttttccgcg ggacgaatgg aagccgtcgc cccggcggac aaacgacccc agcggacgaa
     3121 cgaccccagc ggacgaacga cccctgcgga ctatcgactc ggctccgcca ccggcggcaa
     3181 gacctgatga tgccgatccc tggccagggc agggaaatca ggccggccta gccatttcac
     3241 catcggcacc ttcgaccggt ggcacgatgt gcattgcaaa cgaagcatca gatgccattt
     3301 caatccagaa tatcaggccg agcatcatca ggtgaccagg ctttttccag cgtcggccgc
     3361 aatttcaagt cgtggtgact tgccccgagc cgataggtgg cgattatacg ttccgccgaa
     3421 gggccgctcc gccgagcggc ctcatacggc cgttcgtgcc gtggccatca agacctaacc
     3481 aggagttcga acgcatgcgt cttcgcgggg cgatctttgc caccagcctt ctttccactc
     3541 cggtcgtggc gatggcgcag ccgatcagcg gcctgtatct cggcgccggc gccggattgc
     3601 atgcgccgca aaacccccgc atgaccgcca actcgcccgc gttcggaacc ggccggttgg
     3661 agctgaacga ggattacggc ttcaactcga acctggcggt cggctacggc ctgggcaacg
     3721 ggttccgctt cgagatcggg ggcgacttca tgcgcagcaa cctgcgcagc gttcaggggc
     3781 tgcaaccgac ccgggcagac ggcacgatcc gaacct
//
LOCUS       DASWQN010000442         3044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1362142, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000442 DASWQN010000000
VERSION     DASWQN010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362142"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1899
                     /locus_tag="VGC82_07275"
     CDS             <1..1899
                     /locus_tag="VGC82_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0423108.1"
                     /translation="AIVDLSAAMDQGATLVWPEVRNNLAFDWAIGDKAATDAEFAKAA
                     HVTKMTVVNNRIVVNSIEARAALADFDPATGRWTLYANTQGGWLVKSLIGPVFGTDPE
                     KFRIITPDVGGGFGMKLFLYAEHVLTCYAARKLGRPVKWAADRGEAFLCDTQGRDNLT
                     TAELAIDAEGKFLALRTHNVANMGAYLSTFAPYIPTFAGTSVLASVYGFKAIYANVLG
                     VFTNTVPVDAYRGAGRPESNYLVERIVDTAARELGIDKVELRRRNMVLPEAMPHATPV
                     GKIYDSGDFRIVLDAATKRMDYAGLAARKAEAAARGKRRGLGLAYYLEATGGDPTERA
                     EIRFADDGFVDVYVGTQSTGQGHETAYVQLTAHNLGIDGDKIRIRQGDTDTIPVGGGT
                     GGARSLYSEGQAILLTSESVIRKGKQAAAEELEAAVADIAFAEGRFSIVGTDRGMDIV
                     ALAETQRKKAAAGQKATLLDAAEIAPIDAHTFPNGCHMAEVEIDPETGVVELIRYSVC
                     DDFGKTVNPLIVRGQVAGGVAQGFGQAVLEHTVYDPSSGQLLSGSFMDYAMPRANEFP
                     DIEVELLEIPCASNPLGVKGAGEAGAVGSPPAMINAIIDALSGDGVTHIDMPATPERV
                     WRALARAA"
     gene            2070..2306
                     /locus_tag="VGC82_07280"
     CDS             2070..2306
                     /locus_tag="VGC82_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423109.1"
                     /translation="MNQVSKIEVPVGEATAAALSDERRLGAVGRLIDRLVHPGADDPL
                     IALFERTSADALRAGLTEDEIDAELAVYNAERRV"
     gene            complement(2414..2731)
                     /locus_tag="VGC82_07285"
     CDS             complement(2414..2731)
                     /locus_tag="VGC82_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423110.1"
                     /translation="MFKLLPITFKLLKESSRPKHPDAAPWVEHQQVLIPRYDDGGLGG
                     ERQLQIPVVLGIPAVLDLINRVKPECGRCQQLKNLVTPIRGQNPRKLRPRHNLADFVE
                     DRI"
     gene            complement(2776..>3044)
                     /locus_tag="VGC82_07290"
     CDS             complement(2776..>3044)
                     /locus_tag="VGC82_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423111.1"
                     /translation="WVCPHLAPPVGAWLMVRRLTRRVCPRGTHRPLSTGLHTLGNVRV
                     ILLDANSLRGKGTGADVLDGTCPGSQHFDHATESLCDSRWANFI"
BASE COUNT          570 a          905 c         1055 g          514 t
ORIGIN      
        1 gccatcgtcg atttgtccgc ggcgatggac cagggtgcca cgctggtgtg gcccgaggtg
       61 cgaaacaacc tggctttcga ctgggcgatc ggcgacaaag ccgccaccga cgcggaattc
      121 gccaaggccg cgcatgtgac caagatgacg gtggtcaaca accgcatcgt ggtgaactcg
      181 atcgaggcgc gcgcggcgct ggccgatttc gatcccgcca ccggccgctg gacgctgtat
      241 gccaacaccc agggtgggtg gctggtcaaa agcctgatcg gcccggtgtt cggcaccgat
      301 ccggagaaat tccgcatcat cacccctgac gtcggcggcg gcttcgggat gaagctgttc
      361 ctgtacgccg aacatgtgct gacctgctac gccgcccgca aactcggccg cccggtgaaa
      421 tgggcggcgg accggggcga ggcgttcctg tgcgacaccc agggccgcga caacctgacg
      481 acggcggaac tggcgatcga cgcggagggg aaattcctgg cgctgcgcac gcacaacgtc
      541 gccaacatgg gggcctacct gtccacgttc gcgccgtata tcccgacatt tgccggcacc
      601 agcgtgctgg cgagcgtgta cggcttcaag gcgatctatg ccaacgtgct gggcgtgttc
      661 accaacaccg tgccggtcga tgcctatcgc ggcgcggggc ggcccgaaag caactacctg
      721 gtggagcgca tcgtcgatac cgccgcacgc gagctcggca tcgacaaggt ggaactgcgc
      781 cgccgcaaca tggtgctgcc ggaagcgatg ccgcatgcaa cgccggtggg aaagatatac
      841 gacagtggcg acttccgcat cgtgctggat gccgcgacga aacggatgga ttatgccggg
      901 ctggcggcgc gcaaggcgga agcggcggcg cggggcaagc ggcgcgggct ggggctggcg
      961 tattacctgg aggcgaccgg cggcgatccc accgaacggg cggaaatccg cttcgccgat
     1021 gacgggttcg tcgatgttta tgtcggcacg caatccaccg gccaggggca cgagacggcg
     1081 tatgtacaac tgaccgcgca taacctgggc atcgacggcg acaagatccg catccgccaa
     1141 ggcgacaccg ataccatccc cgtcggcggc ggcaccggcg gtgctcggag tctgtattcc
     1201 gaagggcagg cgatcctgct gacctcggaa tccgtcatca ggaagggcaa gcaggcggcg
     1261 gcggaggaac tagaggcggc ggtggcggat atcgccttcg ccgaaggacg gttctccatc
     1321 gtcggcaccg atcgcggcat ggatatcgtg gcgctggccg aaacgcagcg gaagaaggcc
     1381 gcggcgggtc agaaggcgac gctgctggac gcggcggaga tcgccccgat cgatgcccac
     1441 actttcccga atggctgcca tatggccgag gtcgagatcg atccggaaac cggggtggtg
     1501 gagctgatcc gctacagcgt gtgcgacgat ttcggcaaga cggtgaaccc gctgatcgtg
     1561 cgtggccagg tggccggcgg cgtggcccag gggttcggcc aggcggtgct ggagcacacg
     1621 gtgtacgacc cgtcgtcggg ccagctgctg tccggcagtt tcatggacta cgcgatgcca
     1681 cgggccaacg agtttccgga cattgaggta gagttgctgg aaatcccgtg tgcgtccaat
     1741 ccgttgggtg tgaagggcgc gggcgaggcg ggcgccgtcg gcagcccgcc ggcgatgatc
     1801 aacgcgatca tcgacgcgct gagcggggac ggcgtgactc atatcgacat gccagcgacg
     1861 ccggagcggg tgtggcgggc gctggcgcgg gcggcttagc ggtcggggcg gctagggctg
     1921 ggcaaagttc gggccggaag gcattcatct tgcagcacgt agccagccag ccaggcagcc
     1981 gcttagatcc atgcacctga tacgtggttg cttatgatga cctttctcga aatctctggt
     2041 aagataaaac gcatgaccgg agcgcagcca tgaaccaggt gtcaaagatc gaggttcccg
     2101 tcggagaagc caccgcggct gccctttcgg acgagcggcg gcttggagcc gtcggtcgtc
     2161 tcatcgatcg gcttgtccac ccgggagcgg acgacccgtt gatcgcgttg tttgagcgga
     2221 cgtccgccga cgctctcagg gccggcctta ccgaggacga gatcgacgcg gagctagcgg
     2281 tttacaatgc ggagcgtcgg gtctgagcat ctgcgttatt gatgcgaata cggtcatctc
     2341 ggctgccttg agtccagacg gaacatcctg gcgcgcactc gccgcggcac gggcgcaggg
     2401 tacaattgcg ttgtcaaatt cggtcttcga cgaaatcggc gaggttatgt cgaggccgaa
     2461 gttttcgcgg gttttgaccg cggatcggcg tcacgagatt cttgagctgc tggcagcggc
     2521 cgcactctgg tttgacccgg ttgataaggt cgaggactgc cgggatgcca aggacaaccg
     2581 gtatctggag ctggcgctcg ccgcccaagc cgccgtcatc atatcggggg atgaggacct
     2641 gctggtgctc aacccatggc gcggcatccg ggtgcttcgg cctgctcgat tccttcagga
     2701 gcttgaacgt gatgggcagg agcttgaaca tgatgggcgg caagacgacc gctgaacccc
     2761 gccaaaaacg gcagttcata tgaagtttgc ccagcggctg tcacacaagc tttcggttgc
     2821 atgatcaaag tgctggctgc ccggacacgt gccatcaagt acatcagcgc ccgtgccttt
     2881 gccgcgcagg ctgttcgcgt ccaacaaaat cactcgcaca ttgccgaggg tatgaagacc
     2941 tgtggagagt ggacggtgcg tgccgcgagg gcagacgcgt cgggtcagcc ggcgaaccat
     3001 cagccaggca ccgacaggtg gcgccaagtg cgggcatacc catc
//
LOCUS       DASWQN010000443         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_570282, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000443 DASWQN010000000
VERSION     DASWQN010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570282"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VGC82_07295"
     CDS             <1..448
                     /locus_tag="VGC82_07295"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY0423112.1"
                     /translation="EGDAQGATIYTLSDRGSDAEAERLADAENKADAIGGVNLTEEPT
                     DVADILIDLAQRETRTFSNRFARTLMGEMKRTVRMHKHPLKSAGFKVLKAPDVPSVLI
                     ELGYVSNKDDLEHLVSESWRARTVNSMAQAIDAFFAKRLAIAVPIN"
     gene            652..>1999
                     /locus_tag="VGC82_07300"
     CDS             652..>1999
                     /locus_tag="VGC82_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY0423113.1"
                     /translation="MRLLVRFFGFLFAAGTVLFLVGVAAVAGLIWHYSKDLPEYSQLQ
                     DYEPPVMTRVHAADGALLGEYSKERRLYLPIQAVPKLVVNAFLAAEDKNFYEHGGIDF
                     TGMARAAVVYAQNYGSNRRPQGASTITQQVAKNFLLTNEVSFTRKIKEALLAMRIERA
                     YSKDKILELYLNEIYLGLGAYGVAAASLVYFDKSVNELTVAEAAYLAALPKAPAILHP
                     VRNRERAIERRNYVVDRLLENGWIKQADADKARKDQLVVTSRSNAAHTFAGEYFAEEV
                     RRDVFERYGEKKLYEGGLSVRTTLDPKIQVMARKTMAAGLVTFDEQQGWRGAINKLDI
                     SGDWGVKLADTKSLSDISPWRMAVVLETSDQSARIGFQPGRELGGAVSKERQTGLITL
                     EGVKWAKAAAGPTRFKTPTAVSQVLSPGDIIYADPLIAKDGNVVEGQFRLRQLPEVS"
BASE COUNT          392 a          637 c          638 g          332 t
ORIGIN      
        1 cgaaggcgac gctcagggcg ccacgatcta cacgctgtcc gaccgcggct ccgacgccga
       61 agccgaaagg ctcgccgacg ccgagaacaa ggcggacgcc atcggcggcg tcaacctgac
      121 cgaggagccg accgacgtcg ccgacatcct gatcgatctg gcccagcgcg agacccggac
      181 cttctccaac cgcttcgcac ggaccttgat gggcgaaatg aagcgaacgg tgcggatgca
      241 caagcacccg ctgaaatccg ccggcttcaa ggtgctgaaa gcgccggacg tgccttcggt
      301 gctgatcgaa ctcggctatg tctcgaacaa ggacgacctg gagcatctgg tctcggaaag
      361 ctggcgcgcc cggaccgtca actcgatggc gcaggccatc gacgccttct ttgccaagcg
      421 cctcgccatc gcggtgccga tcaactgaaa gccgtgcccg gaaacggaag ccctagtttg
      481 gccacagcgg cagccctata aaaaccgccg cgaggtcatt ggaatcgatg aaaattcccg
      541 cgcggccgag taggatgggc cggcgcggaa tggcagatcg ctccgcgaca ttgtcgcgct
      601 gacgaaagcg ccggctggtg cgggcgcagg gtttggaacg gatattcgat aatgcgcttg
      661 ctggtacggt tctttggttt ccttttcgcc gcaggaactg tgttgtttct ggtcggcgtc
      721 gccgcggtcg ccgggctgat ctggcattat tccaaggatt tgccggaata ctcccagctc
      781 caggattatg agccgccggt gatgacgcgg gttcatgcgg ccgacggcgc gctgctcggg
      841 gaatattcca aggagcgccg cctctacctg ccgatccagg ccgttcccaa gcttgtcgtc
      901 aacgcgttcc tggcggccga ggacaagaac ttctacgagc atggcggcat cgatttcacc
      961 ggcatggcgc gcgccgccgt ggtgtacgcg cagaactacg gctccaaccg ccgcccgcaa
     1021 ggcgcttcca ccatcaccca gcaggtggcc aagaactttc tcctgaccaa cgaggtgtcg
     1081 ttcacccgca agatcaagga agcgttgctg gcgatgcgga tcgagcgggc ctattccaag
     1141 gacaagatcc tcgagctcta cctcaacgaa atctacctcg gcctcggcgc ctatggcgtc
     1201 gccgcggcct cgctggtcta tttcgacaag tcggtcaacg agctcacggt cgcggaagcg
     1261 gcctatctgg ccgcgctgcc gaaggcgccg gcgatcctgc atcccgtgcg caaccgcgag
     1321 cgcgccatcg agcgccgcaa ttacgtcgtc gaccggctgc tggaaaatgg ctggatcaag
     1381 caggccgatg ccgacaaggc gcgcaaggac cagctggtcg tgaccagccg gagcaatgcc
     1441 gcacacacct tcgccggcga atattttgcc gaggaggtcc gccgcgatgt gttcgagcgc
     1501 tacggcgaga agaaactcta cgaaggcggc ctctcggtaa ggaccacgct cgatcccaaa
     1561 atccaggtga tggcgcgcaa gaccatggcg gcgggcctcg tcacctttga cgaacagcag
     1621 ggctggcgcg gcgcgatcaa caagctcgat atctccggcg actggggcgt gaagctcgcc
     1681 gacaccaaat cgctgtccga tatatcgccg tggcggatgg ccgtggtgct ggaaaccagc
     1741 gaccagtcgg cgcggatcgg cttccagccc ggccgcgaac tgggcggggc ggtcagcaag
     1801 gaacggcaaa ccggcctgat caccctcgag ggtgtgaaat gggccaaggc ggcggcggga
     1861 ccgacgcgct tcaagacgcc gacagcggta tcgcaggtgc tttcgccggg cgacatcatc
     1921 tatgccgacc cgctgattgc caaggatggc aatgtcgtcg agggccagtt ccggctgcgc
     1981 cagctgccgg aggtttccg
//
LOCUS       DASWQN010000444         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_834539, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000444 DASWQN010000000
VERSION     DASWQN010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834539"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VGC82_07305"
     CDS             complement(<1..435)
                     /locus_tag="VGC82_07305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY0423114.1"
                     /translation="MITALLFYVFAAVLLVSAAMVVSARNPVHSVLFLILAFFNAAGL
                     FILLGAEFLAMILVIVYVGAVAVLFLFVVMMLDVNFVELRQGFLNYLPVGALIGVVLL
                     AELVIVLGGWAFAPEAPGLISVPFPGRSVTNTEALGLILYTRY"
     gene            complement(432..920)
                     /gene="nuoI"
                     /locus_tag="VGC82_07310"
     CDS             complement(432..920)
                     /gene="nuoI"
                     /locus_tag="VGC82_07310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY0423115.1"
                     /translation="MAFLDRTVRSLLLGELVAGMGLTLRYFFKQKVTINYPYEKGPIS
                     PRFKGEHALRRYPNGEERCIACKLCEAVCPAQAITIEAEPRVDGSRRTTRYDIDMTKC
                     IYCGLCEEACPVDAIVEGPNFEFATETREELMYNKDKLLANGDRWEPELARRLELDAP
                     YR"
     gene            complement(921..2030)
                     /gene="nuoH"
                     /locus_tag="VGC82_07315"
     CDS             complement(921..2030)
                     /gene="nuoH"
                     /locus_tag="VGC82_07315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY0423116.1"
                     /translation="MYDFFHNTNVGLVILTFVEALALLVPLLIGVAYLTYAERKVLAA
                     MQLRKGPNVVGPFGLGQPFADALKMLMKETIIPTGSNRLLFLMAPILTFTLAMLAWAV
                     IPVNEGWVSADINVGILYLFAISSLGVYGIIIAGWASNSKYAFLGAMRAAAQMVSYEV
                     SMGFVLVSVLLCVGSLNLTDVVHAQRTVWFCIPLFPMFIVFFISALAETNRSPFDLPE
                     GESEIVAGFFVEYSAMSFALFFLGEYANMILMSAMTTILFLGGWLGPFGLLPQIGVIW
                     FILKICVVLFFFLWVRATFPRYRYDQLMRLGWKVFLPLSLLWLVVTAGALMAFGWLPA
                     SPSSVVGDQMSERRTVATEARPALPPFQARAKEFV"
     gene            complement(2035..>2711)
                     /locus_tag="VGC82_07320"
     CDS             complement(2035..>2711)
                     /locus_tag="VGC82_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0423117.1"
                     /translation="QVGALSQDWHGFNVLHTAAGRVGALDLGFVPGPNGKAVDAMLGG
                     GVDLLWLLGADGFDPSRIAANTFVVYQGHHGDAGAQRADVILPGAAYTEKSGTYVNTE
                     GRVQRAFRAVYPPGEAREDWTILRAFSAIIGKTLPYDTIDAVRLRLQQVNPVFGRVGF
                     LPRFGCSDHTGPMGDPAVLSDAPLESAVANYYVTDPISRSSPTMAACVEAQAAPANPP
                     RMMAAE"
BASE COUNT          576 a          873 c          841 g          421 t
ORIGIN      
        1 gtagcgggtg tagaggatca agcccagcgc ttcggtgttg gtgaccgacc ggccggggaa
       61 cggcacggag atgagccccg gcgcttcggg ggcgaacgcc cagccgccga ggacgatcac
      121 cagctcggcg agcagcacaa cgccgatcag tgcgccgacc ggcaggtagt tgaggaagcc
      181 ctggcggagc tcgacgaagt tgacgtcgag catcatcacc acgaacagga acagcaccgc
      241 caccgcgcca acgtagacga tgaccaggat catcgcgagg aactcggcgc cgaggaggat
      301 gaagagcccg gcggcgttga agaacgccag gatcaggaac agcaccgagt gcaccggatt
      361 acgtgcgctg accaccatcg cggccgatac cagcagcacc gcggcgaaga cataaaacag
      421 cagcgcggta atcatctgta gggggcgtcc agttccaggc ggcgtgccaa ctccggctcc
      481 caccggtcac cgttcgccag cagcttgtcc ttgttgtaca tcagctcttc gcgcgtctcg
      541 gtggcgaact cgaagttcgg accttcgacg atggcatcca cgggacaggc ttcctcgcac
      601 aggccgcaat agatgcactt tgtcatgtcg atgtcgtatc gtgttgtgcg gcgcgatccg
      661 tcgacccgcg gttcggcttc gatggtgatg gcctgcgctg ggcaaaccgc ctcgcacagc
      721 ttgcaggcga tgcatcgttc ctcgccgttc gggtagcgcc gcaacgcgtg ctcgcctttg
      781 aagcgcgggc tgatcggacc tttttcgtag gggtagttga tcgtcacctt ctgcttgaag
      841 aagtaacgca gcgtcagccc cataccggcg acaagttcac cgagcagaag gctccgcacg
      901 gtgcggtcga ggaatgccat ctaaacaaac tccttggcgc gtgcctggaa tggcggcagc
      961 gccggcctgg cttcggtcgc gaccgtccgc cgctccgaca tttggtcgcc aaccaccgac
     1021 gacggcgacg caggcagcca tccgaatgcc atcagcgccc cggcggtgac aaccagccat
     1081 agcagcgaca acggaagaaa aaccttccaa cccagccgca tcagttggtc gtagcggtac
     1141 cgcgggaacg tcgcacgcac ccacagaaag aagaacagga ccacgcagat cttgaggata
     1201 aaccagatca cgccgatctg cggcagcagg ccgaacgggc ccagccagcc gcccagaaag
     1261 aggatcgtgg tcatcgcgct catgaggatc atgttcgcgt attcgccgag aaagaacagg
     1321 gcgaagctca tcgccgaata ttcgacgaag aatccggcga cgatttccga ttcaccttcc
     1381 ggcaagtcga acggggaacg gttggtttcc gccaatgccg agatgaagaa cacgataaac
     1441 atcgggaaca acgggatgca gaaccagacc gtgcgctgcg cgtgaacgac gtccgtcagg
     1501 ttcaggctgc cgacacacaa taggacgctc accagcacga agcccatcga gacctcgtag
     1561 ctgaccatct gtgctgccgc gcgcatcgcc ccgaggaacg cgtatttgga gttggacgcc
     1621 cagcccgcga tgatgatgcc atagaccccg agcgaagaga tcgcgaacag atacaggatg
     1681 ccgacgttga tatcggcgct gacccatcct tcgttcaccg ggatgaccgc ccatgccagc
     1741 atcgccagcg taaaggtcag aatcggcgcc attagaaaca ggaggcggtt cgatcccgtc
     1801 gggatgatcg tctccttcat cagcatcttc agcgcgtccg cgaacggctg ccccagaccg
     1861 aaggggccga cgacattcgg ccccttccgc aactgcatcg ccgccaggac cttgcgttcg
     1921 gcgtaggtca ggtaagcgac gccgatcaac aacgggacca gcagcgccag cgcctcgaca
     1981 aaggtcagga tgacaaggcc aacattggtg ttgtgaaaga aatcgtacat cggcctattc
     2041 cgcggccatc atgcgcggag gattggccgg tgccgcctgg gcttccacac acgccgccat
     2101 cgtcgggctg gaccggctga tcggatcggt cacgtagtag ttcgccacgg ctgactccag
     2161 tggcgcgtcc gacagtaccg ccggatcgcc catcgggccg gtatgatcgg agcaaccgaa
     2221 ccgcggcagg aagccgacgc ggccgaacac cggattgacc tgctgcaacc gcagccgcac
     2281 ggcatcgatc gtgtcgtacg gcagggtctt gccgatgatg gcgctgaagg cgcgcaggat
     2341 cgtccaatcc tcccgcgcct cacccggcgg atagacggcc ctgaaggcgc gctggacacg
     2401 gccctcggtg ttcacatacg tgccggattt ttccgtatag gcggcgcccg gcaggatcac
     2461 gtccgcccgt tgcgcgccgg cgtcgccgtg gtggccctgg taaaccacaa acgtgttcgc
     2521 cgcgatgcgg gaggggtcga atccgtcggc cccgagcagc cagagcagat cgacgccgcc
     2581 acccagcatc gcgtccacgg ccttgccgtt cgggccgggg acaaagccga ggtccagcgc
     2641 accgacccgg cccgccgcgg tgtgcagcac gttgaaacca tgccaatcct gcgacagcgc
     2701 gccgacctga g
//
LOCUS       DASWQN010000445         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2285756, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000445 DASWQN010000000
VERSION     DASWQN010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2285756"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2446)
                     /locus_tag="VGC82_07325"
     CDS             complement(<1..2446)
                     /locus_tag="VGC82_07325"
                     /inference="COORDINATES: protein motif:HMM:NF033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HEY0423118.1"
                     /translation="MPRRRALTDAQLENLLALPVIEADLIRHWTLAAADLAVTERRRG
                     GHNQLGYALQLCAFRYPGRLLRPGEAIPETALHFVANQLRVSADVLAAYAARPQTRRE
                     QLDGLREGFGFQMYEHGHGREMLAWLLPVALATTNAVTVAATLMDELRRRKVLAPGSS
                     VIERLIAAVLVVAERHVAGQLTRNLSPAQTEALDALLGSKEDASTSVLAWTRQPPGAP
                     GHKALKRIVDQLARLRTIGLDPAVAEGVHPERLRKLAREGGRFTAQHLRALSPLRRRS
                     TLVATVLDTTARLTDDGVGLFDRAVGRMFRRAEAREENAVLRDARAVNDKVRLFARLG
                     AALIAAKATEGEAGLDGAVVSAIGWEKLVESVAEAERLSRPDKVDLPALATRAWPVLH
                     RLGPLFLDTFELRAVPAAAATLRAVELLREAYKSGSRKWPKGLSVSFLRPAWRKAVRD
                     SGGDGSTEHRRAWEAATLLTLRDSMRSGDIWVEGSRQWRAIEDQLIPPALFAAMREAG
                     PLPVAVPATAEEYLNERRAMLDRRMEEIDAKAAADKLEDVRIKGDELKITPLKAITPE
                     EAEAAAERLYAMAPSARITSVLADVHSWTGFANAFTHLHTGMPADDPRVVLTGVLADA
                     TNLGLTRMAEACSVASYRELAWTAGWHLREDTYRQALAMVVNAQQRQPLAARFGFADV
                     SSSDGQAFLTAGRGQALGAHNARHGHGREPSALFYTHVSSRHAPFHTASIPPSGEAAY
                     VIDGLLYHEADLSIAVHHTDGGGVSDHVFALAHLLGFRFAPRIPNLADRKLYAFGPAS
                     TWAALAPFVAGRPDEKL"
     gene            2540..3157
                     /locus_tag="VGC82_07330"
     CDS             2540..3157
                     /locus_tag="VGC82_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY0423119.1"
                     /translation="MGQRVALYCRVSTADQSCARQERDLAAFAERSGYDVVGTFKETG
                     SGVKLDRAERRKVMALAQARKIDAVLVTELSRWGRSTTDLLATLKELEARRVSVIALN
                     GMAFDLATPHGRMIATVLAGIAEFERELIQERIRSGIAAAKARGKRLGRQPGQRPKSD
                     RLAPKVLALIGQGRSYRLVGRELGLSKNTVAAIAKRSRPTTAPVS"
BASE COUNT          646 a         1204 c         1326 g          638 t
ORIGIN      
        1 tcagcttttc gtccgggcgc ccggcgacga acggcgccaa ggcggcccag gtcgaggcgg
       61 ggccgaaggc gtaaagtttg cggtccgcga ggttggggat gcgcggggcg aaacggaagc
      121 ccagtagatg tgccaaggcg aagacgtggt cgctcacgcc gccgccgtcc gtatgatgga
      181 cggcaatgct gagatccgcc tcgtgataga gcagcccgtc tatgacgtat gccgcttcgc
      241 ccgagggcgg gatggatgcc gtgtggaagg gcgcatggcg cgacgagacg tgggtgtaga
      301 acagcgcgga tggttcgcgc ccgtggccat gccgggcatt gtgggcgccc agggcctggc
      361 cgcgcccggc ggtcaggaac gcctggccgt cggaactgga gacatcggcg aacccgaacc
      421 gcgccgcgag cggctggcgt tgctgggcat tgaccaccat ggccaatgct tggcggtaag
      481 tgtcctcgcg caggtgccag cctgcggtcc aggcgagttc ccggtagctc gcgaccgagc
      541 aggcttcggc catgcgggtc aggccgaggt tggtcgcatc ggccagcacg ccggtcagga
      601 cgacgcgcgg gtcgtcagcc ggcattcctg tgtgcaggtg cgtgaaggcg ttcgcgaatc
      661 cggtccagct atgaacgtcg gccaatacac tggtgatgcg ggcactcggc gccatcgcat
      721 agagccgctc ggcggcagcc tccgcctcct ccggggtgat cgccttgagg ggcgtgatct
      781 tcagttcgtc gcccttgatc ctgacatctt ccagcttgtc cgcagcggcc ttggcgtcga
      841 tctcctccat gcgccggtcc agcatggcgc ggcgctcgtt caggtactcc tccgccgtcg
      901 ccggcacggc taccggcaac ggcccggcct cgcgcatggc ggcgaacaag gcgggcggga
      961 tgagctggtc ctcgatcgcg cgccattgcc ggctgccctc gacccagatg tcgcccgaac
     1021 gcatgctgtc cctcagcgtg agcaaggtgg cggcctccca ggcgcggcgg tgttccgtac
     1081 tcccgtcgcc gccgctgtcc cgcacggctt ttcgccaggc agggcgaagg aagctgaccg
     1141 aaaggccctt gggccatttc cggctcccgc tcttgtacgc ttcacgcaac aactcgaccg
     1201 cgcgcaacgt cgccgcggcg gcgggcacgg cgcgaagctc gaaggtatcc aagaacagcg
     1261 gaccgagacg gtgcagcact ggccaggcac gcgtggccag tgccggcagg tccactttgt
     1321 cagggcggct gaggcgttcg gcttcggcga cgctttcgac gagcttttcc cagccgatgg
     1381 ccgacaccac ggcgccgtcg aggccggcct ccccctcggt tgccttggcc gcgatcaggg
     1441 cggcgccgag cctggcgaag agcctgacct tgtcgttgac ggcacgggcg tctcgcagga
     1501 cagcgttttc ctcgcgggcc tcggcgcggc ggaacatccg gccgacagca cggtcgaaca
     1561 ggccgacccc gtcatcggtc agccgcgccg tggtatccag cacggtcgcg accaaagtcg
     1621 acctgcgacg aagcggcgag agcgcgcgca ggtgctgggc ggtaaagcgg ccgccctcgc
     1681 gggccagttt gcgcagccgc tccggatgca ccccttcagc aacggcgggg tcgaggccga
     1741 tggtgcgcag gcgtgcgagc tgatcgacga tccgcttgag ggccttgtgg ccaggcgcgc
     1801 cgggtggctg acgcgtccag gccagcacac tcgtcgatgc gtcctccttg gacccgagca
     1861 gagcgtccag ggcttcggtc tgtgcggggg aaagattgcg ggtgagctgt ccggcgacgt
     1921 gtcgctctgc cacgaccagg acggcggcga tcaaccgttc gatcacgctc gaacccgggg
     1981 cgaggacttt gcgccggcgc aattcgtcca tcagcgtcgc ggcgacagtg acggcgttcg
     2041 tcgtcgccaa cgccaccggc aagagccaag ccagcatctc acggccgtgg ccgtgctcgt
     2101 acatctggaa gccgaaccct tcccgcagcc cgtcaagctg ctcgcgccgt gtttggggtc
     2161 gggccgcgta cgcggcgaga acgtcggcac tgacacgtag ttggttggca acgaagtgga
     2221 gggccgtctc cgggatggcc tcgccaggcc ggagcaagcg gccgggatag cggaaggcac
     2281 agagctgaag ggcatagcca agctgattat ggccgccgcg gcggcgttcg gtcaccgcaa
     2341 ggtcggcagc agccaatgtc caatggcgga tgaggtcggc ttcgataacc ggaagggcaa
     2401 gcaggttctc aagctgcgca tcggtgagcg cgcggcgtct cggcatagcg ggtgtcccaa
     2461 atagagtgta ggctgattgc tacggttcgt gccccagcag gaacaagggg ttgcgggacg
     2521 ttcggcggga tgagttcaat tgggacagcg cgtcgccctc tattgccgtg tctcgaccgc
     2581 cgaccagtcc tgtgcccgcc aggaacggga cctcgccgca tttgccgaaa ggtccggcta
     2641 cgatgtggtc ggcacgttca aggaaaccgg ctcgggcgtg aaattggacc gggccgagcg
     2701 gcgcaaggtc atggcgctcg cccaggcccg gaagattgac gccgtgctgg taaccgaatt
     2761 gtcccgctgg ggacgcagca cgacggactt gctcgcgacg ctgaaggagc tggaagcccg
     2821 gcgggtgtcg gtgatcgcat tgaacggcat ggcattcgac cttgccacgc cgcacggacg
     2881 gatgatcgcg accgtgctgg caggcatcgc cgagttcgag cgcgagctga tacaggaacg
     2941 cattcgctcc ggtatcgctg cagccaaagc cagggggaaa cgtctcggcc gacagcccgg
     3001 acagcgaccg aaatcggatc gtctcgcgcc aaaggtgctg gcgctcatcg gtcagggccg
     3061 aagttaccgc ctggtcgggc gggaacttgg tttgagcaag aacaccgtcg cagccatcgc
     3121 caaacgaagc cggccaacaa ccgcccctgt cagttgatcc cggccatgtt cctgctacgt
     3181 gccacgaacc gtgctatcct gcccgttcta tggcgtaccc cctctcaggc ccatggcaat
     3241 ttccacatcc tcgacgtctc cggcaatcga gaggagcctc ttgcagaatt cagaccgttg
     3301 cgtgaattta atggtatccg aggatcaggc gggactgggg tgaggctgcg gcttgaataa
     3361 attgaacagg caactggcgg tgaaagcggc aacgccgaac atcaggtgtc agcgctcact
     3421 ggagtggacc cgggtttgcc catggaagcg ggaccggcag atgcgcataa gtttgtggtt
     3481 tgttgctgca atccgttcct tgttcgacgc cgcgtggtga gcacgttgcg tcgatggtcc
     3541 gtcgttcgtt aatcgcgaga cggtttgccg gacagaagtg gcaatccctg cctgaccagt
     3601 ttctttgttt ggctgagggc cgtttcttcg tcccgggctg cgtttcaacc agcgccgcgc
     3661 gccgcggcgg tcaggggagg agcggagcaa cccggagcca cgcgcagcgc cctatagcga
     3721 gcatggcgag gatggcgagc accggacctt gaccgtcgca agcacggcgc aacgatcgtc
     3781 gctggcggga actcgatgcc gctaccaacc ggat
//
LOCUS       DASWQN010000446         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1362439, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000446 DASWQN010000000
VERSION     DASWQN010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362439"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..1606
                     /locus_tag="VGC82_07335"
     CDS             398..1606
                     /locus_tag="VGC82_07335"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0423120.1"
                     /translation="MSAALDLNTTRQSLNLPVNPVAQAQEIASTLRAEAPKIEALGRL
                     TPTVVDALHAHGLYRTLLPKQLNGHDAGLETFVKVMETLAAADASTAWCVGQASGCSM
                     AAAYVDAAIAREIWGDDPHGVLAWGFQLEGRARVVPGGYQVTGKWAFGSGGHHATWMG
                     GHCHVELPDGSLLKDANGNLIERTMLFRQEQINWSSTWNVVGLRGTGSDTYTVTDLFV
                     PDAYTVRRDVDEERRIDEPFFRFTSTSAYACGFAGVSIGIARGMLDELTNLAQTKKPS
                     HTSRALRDSPVMHHLIAENEAKLRSARAFVLETIRDGEDSIRHTGALDTENRVLMRLA
                     TTTAIRRAKEVAECAYHEAGATAIFTSNSFEQKMRDIHASAQQVQGRTGHIEVCGQYF
                     LGIKPSARHI"
     gene            1646..>2487
                     /gene="ltnD"
                     /locus_tag="VGC82_07340"
     CDS             1646..>2487
                     /gene="ltnD"
                     /locus_tag="VGC82_07340"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HEY0423121.1"
                     /translation="MKVGVIGLGNMGMGAALNLLHKGHEVTGCELREETRAAFAAEGG
                     NVVVSPDALPTDLEAVVVFVINAAQTEQVLFGEKGCLARMQRGAVVLACATVAPETAK
                     ALGKKIEQAGFLMLDTPVSGGSAGARAGTMTVMGSGSEAAFAKAGPVLEAISTKVWRL
                     GDEIGVGSTVKMVNQLLAGVHIATAAEALALGIRAGADPETLYKVISESAGSSWMWQN
                     RVPHILAGDDTPSSAVNIFVKDLGIVLDQARALTFPLPMAAAAHQLFLAAAANGQGLK
                     DDSFV"
BASE COUNT          503 a          731 c          817 g          436 t
ORIGIN      
        1 gagactattc gacaaattca taattcgata gatccatgat cgtgcgactt gcggtaaaat
       61 aacaagtttt atgtcaagtt cgtttcaatt aaaccgtccg aacggccaag ctgccaagcg
      121 atcgtcaaac ttcaaataag gccgatgagg cctgttgtat tgacataaaa atgtcaaacg
      181 attgttgggc tggcgattgt tgggctggcg attgttgctc tgacatgggg tggagacacc
      241 tgcccatgaa tgtgaagaac acttcccggc gttcaccttg ccgacaataa gttcgggcaa
      301 agggttcggg tcgcgccatt gacacgcgcc accgcctgac tacaggtcga ccgactatgg
      361 caccggccat aggacaacaa cccgggagac aacagccgtg agcgctgcgc tcgacctgaa
      421 cacgaccagg cagtccttga acctgccggt taatccggtt gcacaggcgc aggagatcgc
      481 gtccacgttg cgtgccgagg caccgaagat cgaggcactc gggcggctga caccgacggt
      541 ggtggacgcg ctgcacgctc acgggcttta ccggaccctg ctgccgaagc aactgaacgg
      601 acacgatgcc ggactggaaa ccttcgtgaa ggtgatggag acgctggcgg cggcggatgc
      661 ctcgaccgcg tggtgcgtcg gccaggcgag tggctgctcc atggccgcgg cctacgtcga
      721 tgccgcgatc gcgcgagaaa tctggggtga tgatccgcat ggcgtgttag cttggggctt
      781 ccagcttgaa ggccgcgcaa gggtagtacc gggtggttac caggtcaccg gcaagtgggc
      841 gtttggcagc ggcgggcacc atgccacctg gatgggcggg cactgccacg tcgagctgcc
      901 ggacggctcg ctgttgaagg acgcgaacgg caacctgatc gagcgcacca tgctgttccg
      961 ccaggagcag atcaactggt cgagcacctg gaacgtggtc gggctgcgcg gcaccggcag
     1021 cgatacctat accgtcaccg atcttttcgt gccggacgcc tacacggtgc gacgcgatgt
     1081 cgatgaggaa cgccgcatcg acgagccctt cttccgcttc acttcgacca gcgcctatgc
     1141 ctgcggcttc gcgggcgtct ccatcggtat cgcccgtggc atgttggatg agctgacaaa
     1201 cctggcgcag acgaagaaac catcccacac ctcccgcgct ttgcgcgaca gcccggtgat
     1261 gcatcacttg atagccgaaa atgaggccaa gttgcgatcc gcccgtgcct tcgtgctgga
     1321 aacgatccgc gacggcgaag acagcatccg gcacacggga gcgttggaca cggaaaacag
     1381 ggtgctgatg cggctggcga ccaccacggc gatccgtcgc gcgaaggaag tggccgagtg
     1441 cgcgtatcac gaggcgggcg ccaccgcgat cttcaccagc aactcgttcg agcaaaaaat
     1501 gcgcgacatc cacgcctcgg cgcagcaggt gcagggccgc accggccaca tcgaagtctg
     1561 cggccagtac tttctcggta tcaaaccatc ggcgcgccat atctagcgca atggcggtcg
     1621 gcggaaatcg cgggaggaga cagtcatgaa agtcggcgtc atcggtctcg gcaacatggg
     1681 catgggcgcg gccctgaacc tgctgcacaa aggccacgaa gtcaccggct gcgaactgcg
     1741 ggaagaaacc cgcgcggcct tcgcggccga gggcggcaat gtggtggtat cccccgacgc
     1801 tttgccgacg gacctggaag cggtggtcgt cttcgtcatc aatgccgcgc aaaccgagca
     1861 ggtgctgttc ggggagaaag gctgcctggc gaggatgcag cggggtgccg tcgtcctggc
     1921 ctgtgccacc gtcgcgcccg aaactgccaa ggccctgggc aagaaaatcg aacaagcggg
     1981 tttcctgatg ctggatacgc ctgtctccgg cggttccgcc ggtgcgaggg caggcacgat
     2041 gacagtgatg ggatcgggat ccgaggcggc gttcgccaag gccggcccgg tcttggaagc
     2101 gatctccacc aaggtctggc gtcttgggga cgaaatcggc gtcggcagca cggtcaaaat
     2161 ggtgaaccag ttgctcgccg gggtgcacat cgcgacggct gctgaggcgc tggcactggg
     2221 catccgcgcc ggagcggacc ctgaaacgct gtacaaggtg atctcggaat ccgcgggcag
     2281 ctcctggatg tggcagaacc gggtgccgca tattctggcg ggtgacgaca cgccatcatc
     2341 ggccgtcaac atcttcgtca aggacctggg gatcgtgctg gatcaggctc gggcgttgac
     2401 cttcccgctg cccatggcgg cggcggcaca tcagttgttc ctggcggcgg cggcgaacgg
     2461 gcagggattg aaggacgact catttgt
//
LOCUS       DASWQN010000447         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2021977, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000447 DASWQN010000000
VERSION     DASWQN010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021977"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VGC82_07345"
     CDS             complement(<1..738)
                     /locus_tag="VGC82_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HEY0423122.1"
                     /translation="MSPQIASYEPGRADFSLPVRINLYSDTQTRPSRAMKEAMMNAEM
                     GDEQGGSDPTVWELCDRTAALLGKEAAMFMPSGTMCNQVAIATHCRPGDEILAHEDAH
                     IQSSEAGGPGAISGVMIRGLPGARGTFTADTLRGAIRPIDRYSPPQRLVEVEQTANKG
                     GGTCWQVADLKAVADVAHEHGMKVHMDGARVLNAAVALGVAAKDVTTDCDTVWLDFTK
                     GLGAPLGAVLAGSRDFIGQAWRWKQRLG"
     gene            840..>1840
                     /locus_tag="VGC82_07350"
     CDS             840..>1840
                     /locus_tag="VGC82_07350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0423123.1"
                     /translation="MKFSNFLFPASMSPADDGRVIDETLREARLTDALGFDTIWLAEH
                     HFDGIVAYVDPVAFAAALATATTRARIGFAVAQMALHHPIRLAEQVALIDHISKGRLI
                     VGLGRGTAYNIYDYQGYGIDHTEAQDRFEEAEAIMFEAWTGKPLRHRGRFFDLNLPEL
                     RPSTYTKPHPYVIRASATEHGMQEIARRGNPFMMNVQSNAVTTQRMDLYRQTLRETGL
                     DQATIAAKVDECWVWRNVFVAETDAEAERIAVPAFIEMHEHRVAMRNRIYAEQNASIL
                     PMPPPGSAPPAHASVENALVCGSPATVAEKLAPLKATGVGGLIMQFRLGTMSYEQTAA
                     "
BASE COUNT          333 a          623 c          577 g          307 t
ORIGIN      
        1 accaagccgc tgcttccaac gccatgcctg gccgataaag tcccgggacc cggcgagcac
       61 cgcccccagc ggcgcgccca gccccttggt gaaatccagc cataccgtgt cgcaatccgt
      121 tgtcacgtcc ttcgccgcga cgccgagggc aacggcggcg ttcagcacgc gggcgccgtc
      181 catgtggacc ttcatgccgt gttcatgggc gacatcggcg acggctttca ggtccgccac
      241 ctgccagcac gtgccgccgc ccttgttggc ggtctgctcg acctccacca gccgctgcgg
      301 tggggaataa cggtcgatcg ggcggatcgc gccccgcagc gtatcagcgg tgaaggtgcc
      361 gcgcgcaccc ggcaggcctc ggatcatcac gccgctgatc gcccccgggc cgccggcctc
      421 gctggactgg atgtgggcgt cctcgtgcgc caggatttcg tcgcccggcc ggcaatgcgt
      481 ggcgattgcg acctggttgc acatggtgcc ggagggcatg aacatcgccg cctccttgcc
      541 cagcaaggcg gcggtccgat cacaaagctc ccacaccgtg ggatccgaac caccctgctc
      601 gtcgcccatc tcggcattca tcatcgcctc tttcatggcg cgcgacggtc gtgtctgggt
      661 gtcggaatac aggttgatgc ggacggggag ggagaaatcc gcccgcccag gttcgtaact
      721 cgcaatctgc gggctcatcg cgacgcgctc catcggcagt tcgttcgctg gtaccagaag
      781 gggcattgcc tgaaaagccg ccacatccga tgctgcggcc tggtttggaa aggcgccgga
      841 tgaaattctc gaactttctg ttccccgcca gcatgtcgcc cgccgacgac ggccgggtga
      901 tcgacgagac gctgcgcgag gctcggctga ccgatgcgct gggcttcgac acgatctggc
      961 tggcggaaca tcatttcgac ggcatcgttg cctatgtcga tccggtcgcc ttcgcggcgg
     1021 cgttggcgac cgccaccacg cgtgcgcgga tcggcttcgc tgtggcacag atggcgctgc
     1081 atcacccgat ccggctggcc gaacaggtcg ccctgatcga ccacatcagc aaaggccggc
     1141 tgatcgtcgg actgggccgc ggcaccgcct ataatatcta cgactatcag ggctacggca
     1201 tcgaccacac cgaggcgcag gacagattcg aagaagccga agccatcatg ttcgaggcat
     1261 ggacaggcaa accgctgcgc catcgcggcc gattcttcga cctgaatttg ccggagctgc
     1321 ggccgtccac ctacaccaaa ccacacccgt atgtaatccg agcctcggcc accgaacacg
     1381 gaatgcaaga gatcgcccgc cgcggcaatc cgttcatgat gaacgtgcaa agcaacgccg
     1441 tcaccacgca gcgtatggac ctgtatcggc aaaccctgcg tgagaccggc ctcgaccagg
     1501 caaccatcgc cgcgaaagtc gatgaatgct gggtctggcg caatgtgttc gtggcggaaa
     1561 cggacgccga agcagaacgc attgccgtgc ccgccttcat cgagatgcat gagcaccggg
     1621 tggcgatgcg caaccgtatc tacgcggagc agaacgcctc catcctgccg atgccgccac
     1681 cgggctccgc gccgccggcc catgcctcgg tggagaatgc gctggtgtgt ggatcgccgg
     1741 cgaccgtggc ggaaaaactg gcgccgctga aggcgacagg cgtgggtggg ctgatcatgc
     1801 agttccgcct cggcacaatg agctacgagc agaccgcggc
//
LOCUS       DASWQN010000448         4141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1890550, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000448 DASWQN010000000
VERSION     DASWQN010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890550"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /gene="rpoD"
                     /locus_tag="VGC82_07355"
     CDS             <1..793
                     /gene="rpoD"
                     /locus_tag="VGC82_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY0423124.1"
                     /translation="VLEDAENLHRRVVQIRKIYNKYEEAKRKLSGGNLRLVVSIAKKY
                     RNRGLSFLDIIQEGNTGLMRAVDKYEYRRGYKFSTYATWWIRQAITRSIADQARTIRI
                     PVHMIETINKLVRTSRQMLHEIGREPAPEELADKLGMPLEKVRKVLKIAKEPISLETP
                     IGDEEDSHLGDFIEDKTAVIPLDAAIQANLREATTRVLSSLTPREERVLRMRFGIGMN
                     TDHTLEEVGQQFNVTRERIRQIEAKALRKLKHPSRSRKLRSFLDD"
     gene            complement(851..1228)
                     /locus_tag="VGC82_07360"
     CDS             complement(851..1228)
                     /locus_tag="VGC82_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423125.1"
                     /translation="MNKVYMTGWPNYSFADGGHSDRIQGGDCHVRIATLGGTLEQFHG
                     DWKLGSLAAAKEVVGGRAKHGHDTSASGGEPAGTFSGHRETALRMRTPDSQQRHTAPI
                     RSRALVDKFLRAVSAHLGSQTPL"
     gene            1880..2797
                     /locus_tag="VGC82_07365"
     CDS             1880..2797
                     /locus_tag="VGC82_07365"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0423126.1"
                     /translation="MDEDLPIVDAHHHIWDLESPLRYPWLISGEHAWLGDYSRIQRSY
                     LPPEYRRDTALHNVIATVHIEAECDRTQQLAETEWLTRIAAETGMPNAIVAHAWVDTP
                     DAEDIIAAQAANPMVRGIRTKPVIASGPNDSVRGEKRSLQDPAWRKGLGLLTRYQLSW
                     DLRVPWYHLEEAAEVCREHPDLRIVLNHTGYPLDRSKEQLAMWKRGMEALASCPHIWC
                     KISGLTVKGKPWTLQVNGAVIRETIGIFGVDRCMFASNYPVDSVKASWDWIFHQFKYV
                     TAGLPAEDRRKLFAENALAFYRIDLPRTP"
     gene            2830..3096
                     /locus_tag="VGC82_07370"
     CDS             2830..3096
                     /locus_tag="VGC82_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY0423127.1"
                     /translation="METTRLSSKGQIILPKAVRDTHHWAPGTDFSVEDTGDGVVLRPI
                     KTGQPTRLDDLVGSLRVKGPARTIEEMDSVIDAELRDRRDRGRY"
     gene            3077..3481
                     /locus_tag="VGC82_07375"
     CDS             3077..3481
                     /locus_tag="VGC82_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0423128.1"
                     /translation="MIAADTNVIIRLLTDDEPRQTEQSRRLFETETIFLPKTVLLEAE
                     WVLRRLYRLDPLPVAKALDGLVSLPNVRCEDEAAVRQALDWKREGMDFADALHLASSR
                     TASRFATFDLAMIKSWRMIKTAAKSDLAVSEP"
     gene            3569..>4141
                     /locus_tag="VGC82_07380"
     CDS             3569..>4141
                     /locus_tag="VGC82_07380"
                     /inference="COORDINATES: protein motif:HMM:NF004779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HEY0423129.1"
                     /translation="MDLNLRGRTALITGASKGIGMASAECLAAEGVNVILVSRTQADL
                     EAARSGIAARYNVNAQIHAYDLSDSRNVDRLVEQHPDIDILVNNAGAIPGGDLQAITE
                     DRWRAAWDLKVFGYINMCRRFYAEMKSRRRGVIINILGMAGEKMDRTYIAGSTGNAAL
                     MAFTKALGGSAGDDGLRVVGINPGAIATDRL"
BASE COUNT          881 a         1257 c         1229 g          774 t
ORIGIN      
        1 tgttctggaa gacgcggaaa acctgcaccg ccgcgtagtg cagatccgta agatctacaa
       61 caagtacgaa gaagccaagc gcaagttgag cggtggcaac ctgcgtctcg tggtgtcaat
      121 cgccaagaag taccgcaatc gcgggctgtc gttcctggac atcattcagg aaggcaacac
      181 gggtttgatg cgcgcggtcg ataagtacga gtatcgccgc ggatacaagt tcagcaccta
      241 tgcgacgtgg tggatccgtc aggcgatcac gcgttcgatc gccgatcagg cgcgcaccat
      301 ccgcatcccg gtgcacatga tcgaaacgat caacaagctg gtgcgtacgt cgcggcagat
      361 gctgcacgaa atcggccgcg aaccggcacc ggaagaactg gccgacaagc tgggaatgcc
      421 gctggagaaa gtccgcaagg tcctgaaaat cgccaaggag ccgatcagcc tggaaacgcc
      481 gatcggcgac gaggaagatt cgcatctggg tgatttcatc gaggacaaga ccgcggtcat
      541 tccactggac gcggcgattc aggcgaactt gcgggaagcc acgactcgcg tgctgtcgtc
      601 gctgaccccg cgtgaggagc gtgtgctgcg catgcggttt gggattggaa tgaacaccga
      661 ccacacgctg gaagaagtcg gtcagcagtt caacgtgacc cgcgaacgta tccgccaaat
      721 cgaagcgaag gcacttcgga aattgaagca cccgagccgg agccggaagt tgcggagctt
      781 cctggacgac tgagcgaaat ttttcaggtg ggattaccgg aaaggttaat cacgccctca
      841 aacaacgggc ttagagcggg gtctgcgaac cgaggtgagc agaaaccgct ctaaggaatt
      901 tatccacgag cgctctgctg cgaatcgggg ctgtgtgccg ctgttggctg tccggcgtgc
      961 gcatccttag agcagtttca cggtgaccgg aaaaggtgcc ggccggttcg ccgcccgacg
     1021 ccgatgtgtc atggccgtgc ttggcgcggc cacccacgac ttcctttgca gccgcgagag
     1081 aacccagttt ccagtcaccg tgaaactgct ctaaagtccc accgagcgtg gcaatccgaa
     1141 catggcaatc gccgccttgg atgcgatcgg aatgaccccc gtcagcgaaa ctgtaatttg
     1201 gccatccagt catgtaaact ttattcatga atttcccgcg cattaccccc gcgcattacc
     1261 attgacccga ccatatgcac cggcaaaccc tgcatccgcg gcctacggtt cccggttgca
     1321 cgttggctcg gacggcttgc cgccggacag tcgcgggaag atatcctcgc cagttatccc
     1381 taccttgaag ctgaggatat caacgagccg cttcggtacg ccgcatttct ggcggaagac
     1441 gagacgctcg aacttgtgca gtgacgtttc tggtcgacat gcctttgccg cctgcattgg
     1501 cgctctggtt gaaaagccag gggcatgatg ccgtacatgc gctggacctc caattggaca
     1561 gagcgcccga cgccgaaaat tatcggacgc gctctgacga aaaagcgaac catcgtcact
     1621 gcggatctcg attactccga ttacttgccc tcagccgagc gaccgaacca agccttgtgc
     1681 tgtttcgggc gcgaattggg cagagaccac ggtgatcgca agaatgaacg acatactccg
     1741 cagtttaggc gtccaagaaa tcgaacagag cattctggtg gtcgatcctg accggatccg
     1801 gcgccggcgt ttgccgatcg aatagccgac gcagcgccac ccgtagccta accattaaat
     1861 ccctgcttac gtctccggca tggacgaaga tctccccatt gtcgatgcgc accaccatat
     1921 atgggacctc gaatcccctc tccgctaccc atggctgatc tccggcgagc acgcttggct
     1981 gggtgactac tcgcgtattc agcgcagcta cctgccgccg gagtaccgtc gggatacggc
     2041 gctgcacaac gtcatcgcca cggttcatat cgaggctgag tgcgaccgta cccagcaact
     2101 tgcggaaacg gaatggctga cgcggatcgc cgccgaaacc ggcatgccga atgccatcgt
     2161 cgcccatgcc tgggtcgata cgccggacgc cgaggacatc attgccgccc aggcggctaa
     2221 cccgatggtc cgcggcattc gcaccaaacc cgtcatcgcc tctggcccaa acgattctgt
     2281 gcgcggcgag aagcgcagcc tgcaggaccc tgcctggcgg aaaggccttg ggttgctgac
     2341 cagataccaa ctgtcctggg atttgcgcgt gccctggtac catctggaag aagccgccga
     2401 ggtctgccgc gagcatcccg acctgcggat cgtgctgaac cacaccggct atccgctgga
     2461 tcgttccaag gagcaactcg cgatgtggaa gcgcggcatg gaggcgctgg cctcctgccc
     2521 gcatatctgg tgcaagattt ccggcctgac ggtgaagggc aaaccctgga cgctgcaggt
     2581 caacggcgcg gtgatccgcg aaaccatcgg catattcggc gtggaccgct gcatgttcgc
     2641 atccaactat ccggtggaca gcgtaaaagc gagctgggat tggattttcc accagttcaa
     2701 atacgtcacc gcgggcttgc cggccgagga tcgcaggaaa ctgtttgccg agaacgcatt
     2761 ggcgttctac cggatcgatc tgccgcgaac gccttgacgt tcgtcttaca acgtcttaca
     2821 aggaccttca tggaaaccac ccgactctct tcgaaaggac agatcatcct gcccaaggcg
     2881 gtgcgggata cgcatcattg ggcgccgggc acggatttct cggtggaaga taccggcgac
     2941 ggggtggtgt tgcggcccat caagaccggc caacctacgc ggctggacga tctcgtcggc
     3001 agtctgcggg tcaaagggcc tgcccggacc attgaagaaa tggactccgt catcgacgcc
     3061 gaactgcgcg accgtcgtga tcgcggccga tactaacgtt atcatccgtc tgctgaccga
     3121 cgacgagcct cggcagacgg aacagtcccg ccggttgttt gagaccgaga cgatcttcct
     3181 tccgaagacg gtgctccttg aagccgagtg ggtgcttcga agactatatc gcctcgatcc
     3241 attgccggtc gccaaggcgt tggatggctt ggtctcactg ccgaacgtgc ggtgcgagga
     3301 cgaagcagcg gttcgccagg ccttggactg gaagcgtgaa ggcatggatt tcgccgatgc
     3361 cctccatctg gcttccagcc gaacggcctc ccggttcgcc acgttcgacc tcgccatgat
     3421 caagagctgg cgcatgatca aaaccgcggc caagtcggat ctggctgtct cggaaccctg
     3481 attgccgaaa tcccagattg ccgaaatcct agattgccga aattccgggg ccgctctatc
     3541 cggtggtcaa acaacgggag aagcggcgat ggacctgaac ctgcgcggac gaaccgcctt
     3601 gatcacgggt gcctcgaagg gcatcggcat ggccagcgcg gaatgcctgg cagcggaagg
     3661 cgtcaacgtc atcctggttt cccgcaccca ggccgacctg gaggcggcgc gatccgggat
     3721 tgccgctcga tataacgtca acgctcagat ccacgcttac gatctatccg acagccgcaa
     3781 tgtggaccgg ttggtcgaac agcatcccga tatcgacatc ctggtgaaca acgccggcgc
     3841 cattcctggt ggtgaccttc aggccatcac cgaggatcgc tggcgcgccg cctgggacct
     3901 gaaagtcttc ggctacatca acatgtgccg ccgcttttac gccgaaatga aatcgcggcg
     3961 gcgcggcgtg atcatcaata tcctcggcat ggccggcgag aagatggacc ggacctatat
     4021 cgccggatcc actggcaacg cggcactgat ggccttcacc aaggcattgg gcgggtcggc
     4081 gggcgacgac gggctgcgtg tcgtcggcat caatcctggc gccatcgcca ccgatcgcct
     4141 g
//
LOCUS       DASWQN010000449         3686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_966200, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000449 DASWQN010000000
VERSION     DASWQN010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966200"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /gene="mnmA"
                     /locus_tag="VGC82_07385"
     CDS             <1..942
                     /gene="mnmA"
                     /locus_tag="VGC82_07385"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY0423130.1"
                     /translation="CCAGQDIHDARRVADRLGIPHYTIDAEERFTRSVIARFADSYAS
                     GETPVPCISCNQTVKFADLSALARDLGAERLATGHYVRRVDGPQGAELHRAADPARDQ
                     SWFLFATTRRQLDLCLFPLGGMTDKRAVRDVALRLGLPVAEKPDSQDICFVPSGAYAD
                     VVAKFRPDALAPGQIVTTGGRVVGNHDGIARYTVGQGKRLGDAATLDGTRQMVVATEP
                     GRRRVVVGPRGTGTKTMRLREVNWLLPMPSHDLRCAVKLRARDEPRPATVRTDEGGTV
                     VVLDEAALPAPGQACVFYRGDRILGGGIINRMDAPDP"
     gene            1019..1445
                     /gene="rnpB"
                     /locus_tag="VGC82_07390"
     ncRNA           1019..1445
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGC82_07390"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1848..2327
                     /locus_tag="VGC82_07395"
     CDS             1848..2327
                     /locus_tag="VGC82_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY0423131.1"
                     /translation="MALFIGTHQSKRDAKGRVSIPAQFRSVLKQNSQAGKDAPVAAMY
                     LRPSHQNPCIEGWTELGFEALSTPMAEGYNQFSSEYEDFVVALFGNARLLETDTEGRI
                     MLPPNLIEYAGLKQDVVFIGARNTFQIWEPEAGERRLAEARERTRSAGFTLRTGGLA"
     gene            2324..3265
                     /gene="rsmH"
                     /locus_tag="VGC82_07400"
     CDS             2324..3265
                     /gene="rsmH"
                     /locus_tag="VGC82_07400"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY0423132.1"
                     /translation="MSFHVPVMLDEVLATLAPKDGGIYLDGTFGGGGYARAILEHARC
                     TLWAIDRDPEAIDRGAELVALFPGRLHLLHGQFGDMVALLDQAGVSELDGVVLDLGVS
                     SFQLDDAGRGFSFRFDGPLDMRMGRHGTSAADLVNTLPERALADVLYELGEERASRRI
                     AHAIVAARLEAPIETTGRLADIIRSVLPPDRSGNHPATRSFQALRIKVNDELRQIEAA
                     LEQAIGLLSPGGRLVVVAFHSLEDRIVKRFMGTSAGRMGSPSRHNPAGLVTREAPNFR
                     LLTAKALRPGADETRRNPRSRSARLRALERLDKGRER"
     gene            3262..>3686
                     /locus_tag="VGC82_07405"
     CDS             3262..>3686
                     /locus_tag="VGC82_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423133.1"
                     /translation="MIRPLTIVTFVMACGSGLYLYQSKHEVQVLDRTIERTVHDTNAL
                     REQSHLLAVEWTMLNDPERLRQFSDTYLNLKTINPPQFASLGDLNNRLPQPRAEAPPH
                     GAGEDEATSVETTAQPVPEPDQGIAAEEVLPVPPIPNMHP"
BASE COUNT          690 a         1208 c         1197 g          591 t
ORIGIN      
        1 tgctgtgccg gccaggatat ccatgatgcc cgccgggtgg cggatcgact cggcatccct
       61 cactacacga tcgacgccga ggaacgcttc accaggtcgg tgatcgcccg gttcgccgac
      121 agctatgcca gcggtgaaac cccggtgcct tgcataagct gcaaccagac cgtgaagttt
      181 gccgacctgt cggccctcgc acgcgatctc ggcgccgaac gcctggcgac cggccactat
      241 gtaaggcgtg tcgatgggcc acagggggcg gaactgcaca gggctgccga tccggcgcgc
      301 gaccagagct ggttcctgtt tgccacgacc cgccggcaac tcgacctgtg cctgtttccg
      361 ctgggcggca tgacggacaa gcgggcggtg cgcgacgtcg cgctgcggct gggtctgccc
      421 gtggctgaaa agccggacag tcaggatatc tgcttcgtgc cgtcgggcgc atacgcggac
      481 gtcgtggcga agttccgccc tgatgccctg gcgccgggtc agatcgtaac caccggcggc
      541 cgggtcgtcg gcaaccatga cgggatcgcc cgctataccg tgggacaggg caagcgactt
      601 ggcgacgcgg cgacgctgga cggaacccgt cagatggtcg ttgccacgga gcccggacga
      661 cggcgagtcg tcgtgggacc gcgtgggacg ggaaccaaaa ccatgcgcct gcgcgaggtg
      721 aactggctgc tccccatgcc ttcccatgac ctccgctgtg cggtcaagct gcgggcccgc
      781 gacgaaccgc ggccggcgac ggtgcggacg gacgagggcg gcaccgtcgt cgttctcgac
      841 gaagccgccc tgccggcgcc gggacaggcg tgtgttttct accgcggcga ccgcatcctc
      901 ggcggcggca tcatcaaccg catggatgca ccggatccgt aagcggggtg ccgccggcac
      961 ttaagctggc cgccgaacat gcttgaccgg cagccccgcc gggcgcacat ccccggcgcc
     1021 agacagctgg acgaccgctg cctgcggggc aatccgccgg tggaggaaag tccgggctcc
     1081 atgggaacac ggtgccggct aacggccggc gggggcgacc ccagggacag tgccacagaa
     1141 aacaaaccgc ccgtgcaagg ccggcttgcg caagcagact ggccccgcgg gcaagggcga
     1201 aacggtgcgg caagagcgca ccgcgcgcct ggcaacaggg gcggcacggt aaaccccacc
     1261 gggagcaaga ccgaataggg gcggcagtga cgttacggtc cagaacaccg tgatgattac
     1321 aggggcgttc ccgtcccgcc gcccgggttg gtcgcgtgag gcgcatcgcg agatgcgtcc
     1381 cagaggaatg gtcgtccaac cccgcaaggg gcggacagaa cccggcttac aggctgtctg
     1441 gcctttcccg ccgagtcgcc cgctcacgcg ctccaggcac tcactcatac tgatgcgccg
     1501 gaggcaaccg ttgcggttca gtggtttgaa aaactctcca caaagttgtc cacaggaacg
     1561 aaaggcgaat agtttcttgc catagatttg ggtaggggtg aatcggatcg ccaccggtaa
     1621 gtgtctgatc aagccgagaa attatcggtc gaaaccttca gtaaaggagg caaaaccttt
     1681 gacctcccaa gctcgcccat gctatcccat ctgcacccaa gttggatggt catccacaag
     1741 ggcctccaac cggatgcggc ttatcagtcg aacacctgca accggtgatt atcgcgtctg
     1801 gctcgcccaa gacacttacg ctacgcagga aaggcaaacg ggtccgaatg gcccttttta
     1861 tcggcacaca ccaaagcaag cgagacgcca agggccgtgt gtcgatccct gcgcaatttc
     1921 gttctgttct gaaacagaac agccaggcgg gtaaggacgc gccggtcgcc gcaatgtacc
     1981 tgcggccgtc gcatcaaaac ccctgcatcg agggttggac ggaactgggg ttcgaggcgc
     2041 tcagcactcc gatggccgaa ggctacaacc aattcagctc cgagtacgag gattttgtcg
     2101 tggcgctgtt cggcaacgcc cgcctgctgg aaaccgatac ggaagggcgg atcatgctgc
     2161 ccccgaacct gatcgagtac gccggcctga agcaggacgt ggtcttcatc ggcgcgcgca
     2221 acacattcca gatctgggaa cccgaggccg gcgaacggcg cctggccgag gcgagggaaa
     2281 gaaccaggtc agccggcttc accctgcgaa cgggcggact ggcatgagct tccatgtccc
     2341 cgtaatgctc gacgaagtcc tggcgacgct ggcgccgaag gacggcggca tctacctgga
     2401 cggcacgttc ggcggtggcg gctatgcgcg cgccatcctg gagcacgcac gctgcacgct
     2461 ttgggcgatc gaccgcgatc ccgaggcgat cgatcgtggc gctgaactgg tggcgctttt
     2521 tcccggtcgg ctgcacctgc tgcatgggca gttcggcgac atggtggctc tcctcgatca
     2581 ggcgggcgtc agcgaactcg acggcgtggt gctcgacctc ggcgtgtcct cgttccagct
     2641 cgacgacgcc ggtcgcggct tctccttccg cttcgacggc ccgctcgata tgcgcatggg
     2701 aaggcatggg accagcgcgg ccgatctggt caacaccttg cccgaacgcg cgctggcgga
     2761 cgtgctgtat gaactgggtg aggaacgcgc ctcgcgccgg atcgcccatg ccatcgtcgc
     2821 cgcacggctg gaagcgccga tcgagaccac cggccggctg gcggacatca tacgttcggt
     2881 actgcccccg gaccggtcgg gcaatcatcc ggcgacgcgc agcttccagg cgctgcgcat
     2941 caaggtcaac gatgagctgc ggcagatcga agctgccctg gaacaggcga tcgggttgct
     3001 gtcgccaggc ggcaggctcg tggtcgttgc gttccacagc ctggaggatc gcatcgtgaa
     3061 gcggttcatg ggcacttcgg ccggacgcat ggggtcaccg tcccggcaca atcccgccgg
     3121 actcgtaaca cgcgaggcgc ccaattttcg tctgctgacc gcgaaggcgc ttcgtcccgg
     3181 tgctgacgaa acacgacgca atccacgctc tcgcagcgcc cggctgcgtg cccttgaacg
     3241 tctcgacaaa ggccgcgaac gatgatccga ccgctcacca ttgtcacctt cgtcatggcc
     3301 tgtggctccg ggctttatct ctaccagtcc aagcacgagg tgcaggtgct cgaccggacg
     3361 atcgaacgaa ccgtccacga caccaacgcg cttcgcgagc aaagccacct gctggcggtg
     3421 gaatggacga tgctgaacga tccggaacgg ctgcggcagt tctccgacac ctacctgaac
     3481 ctcaagacga tcaatccccc gcagttcgcc agcctcgggg atctcaacaa ccggttgccg
     3541 caaccgcgcg cggaagctcc gccgcatggt gccggtgagg acgaggcgac atcggtcgag
     3601 accacggcgc agccggtgcc ggagccggat cagggtatcg ccgccgagga ggttctgccg
     3661 gttcccccca ttccgaacat gcaccc
//
LOCUS       DASWQN010000450         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_307068, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000450 DASWQN010000000
VERSION     DASWQN010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307068"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VGC82_07410"
     CDS             <1..1038
                     /locus_tag="VGC82_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0423134.1"
                     /translation="DRGVDGFRVDVLWLLIKDRQFRDNPPNPLWQANQAGIDRLMQQY
                     SADQPEIQEVVEEMRAVLDTYPDRVLIGEIYLPVERLVTYYGKDMKGAHLPFNFQLIF
                     AAWNAREIARTVAEYEAALPAGGWPNWVLGNHDQKRIATRVGTAAARVAAMLLLTLRG
                     TPTLYYGEELGLENVPIPPDRVQDPWERNEPGLGLGRDPARTPMPWDGSGNAGFTIGT
                     PWLPLNADHAAANVATLTGEPHSMLSLYRRLLALRRGLAALHSGAYVESHTEGDVLAF
                     ERRHDPGRRLLVALNFGRLPQRLRLPHDALDARLLLSTKLDREGEAMHATIDLRPEEG
                     VILDLSPHQQA"
     gene            complement(1044..>1691)
                     /locus_tag="VGC82_07415"
     CDS             complement(1044..>1691)
                     /locus_tag="VGC82_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0423135.1"
                     /translation="RGIRRRLAPMLNGDRPRLELAYSLLMTLPGTPLIRYGDEIGMGD
                     DLRLPERTCARTPMQWSSEPHGGFTLSDDPVLPVIDQGPYGFEHVNAAAQRRDPNSLL
                     NWTERIMRMRKELPEIGWGDFTVLPSGTTEVLVLRYDWRNNAVLFVHNFAAAAREISL
                     ELEPTEGEKRDLVNLLSEDHNYADASGTHHIRVEPYGYRWYRVGGLDYLLKRSES"
BASE COUNT          326 a          531 c          518 g          316 t
ORIGIN      
        1 gatcggggtg tggatggctt ccgcgtcgac gtgctttggc ttctgatcaa ggacaggcag
       61 ttccgcgaca acccgccaaa tccgctgtgg caggcaaatc aggccggcat cgatcgcctg
      121 atgcagcagt actccgccga ccaaccggag atccaggagg tcgtcgaaga gatgcgggcg
      181 gtgctggata cctatcccga tcgggtgctc atcggcgaga tctacctgcc tgtcgagcgc
      241 ctggtcacgt attacggcaa ggacatgaaa ggcgcgcatc tgccatttaa cttccagctc
      301 atttttgccg cctggaacgc gcgggaaata gcccgcaccg tggcggaata tgaggccgcc
      361 ttgccagccg gcggctggcc caactgggtc ctcggcaacc acgatcagaa acgcatcgcg
      421 acacgcgtcg gaaccgcggc ggcgcgcgtt gcggcaatgc tgctactgac cctgcgcggt
      481 accccaacgc tctattacgg cgaagaactc ggactggaga acgtgccaat cccgcctgac
      541 cgcgtgcagg acccgtggga aaggaatgaa ccaggcctgg gtctcgggcg tgatccggca
      601 cgaacgccaa tgccgtggga cgggtcgggg aatgccggat tcaccatcgg cacgccatgg
      661 ctgccactca atgccgatca cgcggccgcc aacgtggcaa cgctgaccgg cgaaccgcat
      721 tcgatgctgt cactctaccg ccggcttctt gcgctgcgcc ggggcctggc ggcacttcat
      781 tcgggcgcct atgtcgaaag ccacacggaa ggcgatgtcc ttgcgttcga acgtcgccat
      841 gacccgggtc gccgcctgct ggtggcgttg aattttggca ggctaccgca gcgcctgagg
      901 cttccgcacg atgcactgga cgcgcgcctg ctgctttcga cgaagctcga ccgagagggc
      961 gaggccatgc acgcaacgat cgatctgcgt ccggaggaag gcgtcatcct ggacctttcg
     1021 ccgcaccaac aggcataatc gagctaactt tcgctccgtt tcagaagata atccaggccg
     1081 ccaacccgat accagcggta gccgtatggc tccacgcgaa tatggtgcgt tccggaggca
     1141 tccgcataat tatggtcttc ggaaagcagg ttgacgaggt cccgcttttc accctcggtg
     1201 ggttccagct caagcgaaat ttctcgtgcc gcggctgcga aattgtggac gaacaggacc
     1261 gcgttgtttc gccagtcgta ccgtagaacc agcacctccg ttgtgcccga cggcaatacc
     1321 gtaaaatccc cccagccaat ttcgggtaat tccttgcgca tgcgcatgat gcgttcggtc
     1381 caattaagta atgaattcgg gtcgcggcgt tgcgctgccg cgttgacatg ctcgaagccg
     1441 taagggcctt ggtcgatcac cggcagaacc ggatcatcgc tcagcgtaaa gccgccgtgc
     1501 ggctcgctgg accactgcat cggggtgcgg gcgcaggtcc gctccggtag gcgcaaatcg
     1561 tctcccatgc cgatctcgtc gccataacgg atcaacggtg tgcccggcaa tgtcatcagc
     1621 aggctgtagg ccagttcaag cctgggccgg tcgccattca gcatgggtgc cagacgccgc
     1681 cggattccgc g
//
LOCUS       DASWQN010000451         6276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1098255, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000451 DASWQN010000000
VERSION     DASWQN010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098255"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VGC82_07420"
     CDS             <1..270
                     /locus_tag="VGC82_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423136.1"
                     /translation="MIACRGKLQREGEVLHVIAEHLTDLSDMLRGVGERDDKPFPLPY
                     GRGDEARHGSTPDSREKVVGTRDIYIRDLRVVPGKGIKVPTRDFR"
     gene            506..1414
                     /locus_tag="VGC82_07425"
     CDS             506..1414
                     /locus_tag="VGC82_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEY0423137.1"
                     /translation="MEAIVGSGKHTYKVHEDWARLPAHVELKPAAVAVGPQDRVYCFN
                     RVTEHPVVVFDQDGTFLSSWGAGMFKFPHAIRFDAQGFVWVTDGHHQQFMKFTPDGQL
                     LETIGIKGQRSDTGVPDDDFTSVAWKKVTHGGGPFNLPTDIAFAPDGSMFMTDGYGNA
                     RVHKFSADAKHLFSWGEPGTAPGQFNLPHGIWIDRRGRLLVADRENDRVQIFDQEGHL
                     LEIWPTELIGPAFFYVDADDIVYVAEHNGGMISILTLDGERLARWGAPIHRSCHGIWG
                     DSHGDIYVVQPGEWGRVRRVVKFERQ"
     gene            complement(1504..3000)
                     /locus_tag="VGC82_07430"
     CDS             complement(1504..3000)
                     /locus_tag="VGC82_07430"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0423138.1"
                     /translation="MREIGHFIGGKHVAGTSGSFGHVFNPASGEVSAKVAMANASEVD
                     VAVAAANAVWPAWANTPPLRRARVMFKLKELIERDRREMSAIITDEHGKVLSDADGEV
                     QRGLEVVEFACGIPHLLKGEYTDQVGTGIDAWSIRQPLGVVAGITPFNFPIMVPLWMI
                     PVALATGNCFILKPSEKDPSVALKLAELLKEAGLPDGVFSVVQGGREAVETIIAHPGI
                     AAISFVGSTAIAETIYRGGTSHGKRVQALGGAKNHLVVMPDADIDDAVDALMGSAYGA
                     AGERCMAVSVAVTVGSAGDALMSKLAPRVRALKVAPGVDPEAEMGPLVTREHLEKVRG
                     YVDLGVQEGADLVVDGRGLKLQGYENGFFLGGCLFDNVTPDMRIYKDEIFGPVLSVVR
                     APDVATATRMINAHDYGNGVALFTNDGGVARDFTQAIQIGMVGINVPIPVPMAFHSFG
                     GWKHSLFGDHHIYGPEGVRFYTRYKAVTQRWTAGTRRGSEFTMPTLGR"
     gene            complement(3282..4085)
                     /locus_tag="VGC82_07435"
     CDS             complement(3282..4085)
                     /locus_tag="VGC82_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423139.1"
                     /translation="MSDKRNFVLIPDDAPPITPAPRGGILARLRAMATTAVAAAPSTA
                     ASLLSALGHAEPPAESGVPARPRLVFAVDATASREPAWAAARQVTDALVKALPGELDV
                     ALAVHGGSRVHTFTAFTNHASTLRDRAAGVACEAGLTRLLPILLASLKQPAVRVVIYI
                     GDVFEESMSQGRRLADALGVQGTKLIVLHDTADPAARRDAEVFWDLAKRTGGCVLPFD
                     ASAPGRLRDLLSAVAVYAVGGEKLLRERRHELPGAVVLLEHLGDRRGGK"
     gene            4455..>6276
                     /locus_tag="VGC82_07440"
     CDS             4455..>6276
                     /locus_tag="VGC82_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY0423140.1"
                     /translation="MQWPGLRDWMFASKTFVAAVLALYIALSLGLDRPYWAMASAYIA
                     SQPLSGATRSKAVYRLCGTVAGATAAVLLVPAFAEAPPLLSLVLALWVAGCLYLSLLD
                     RTPRSYACMLAGYSAAIIGFPSVVAPEAIFDTALTRVEEIAIGVSLATLVAGIVFPRP
                     VGPALITRIDAWFENARRWSIEALSDRDTAAMEARRLAADGVEINLLASHLSYDATAW
                     ETVHFGQLRARMIMLIPILASIADRMAALRARMPQPAAALAADLVEWLESGVSGDAQS
                     LSARIDEMAASLSRKSDWDALLLTSLLIRMRELTGILTDCATLRRQMASPVRPAEAAL
                     HFAPEAVAAPAWHRDHGMALLSAAACAVAILLSCTIWIATAWNEGYIAAEMVAVACSF
                     FAAQDDPVPAIIQFLGWSLVAVVMDGILLFGVLPAASDFITVVLALAPLFLIGGVLIA
                     MPATANGGRAFTANAATLLALQSAYTADFPAFVNSGLGFLLGLALAATVTRLIRSVGA
                     GFGVYRLLRIIWLDLAIAAERRGNRDRARYAGLMLDRLGLIAPRLAETEPDAAPTDAL
                     TDIRIGFNIIDLRRARHVVALPVRERIDAMLDALANAYRSR"
BASE COUNT         1094 a         2106 c         1934 g         1142 t
ORIGIN      
        1 atgatcgctt gtcgcggaaa gctgcagcgg gaaggcgagg tgctgcatgt catcgccgaa
       61 catttgactg atttgtccga catgctgcgt ggcgtggggg aacgcgacga caaacccttt
      121 cccctgcctt acggccgcgg cgacgaagcc aggcatggca gcaccccgga ttcgcgtgag
      181 aaagtggttg gcacccgcga catctacatc cgggatctac gggttgtccc gggaaagggg
      241 atcaaggtgc cgacgcggga tttccgctga caggccggaa ggccgaggaa gcgcttcatc
      301 ccatagtcca acctgttgct tgaccgccta atcagcggcc accgatagta ccggcaacaa
      361 caatacaact gacgaatgta cccgtgcagc cggaggatgc cgactccagc ttgacatggg
      421 caccattcat ccgatgctga acgcgacatc gaacagatag cggcaccggc aaacggtcaa
      481 gtcgaagtca tgggagggga ttgtggtgga agcaatcgtt ggcagtggca aacacaccta
      541 caaagtgcac gaggactggg ctcggctgcc cgcacatgtg gaactgaagc cggccgcggt
      601 ggcggttggt ccccaggatc gggtctattg cttcaaccgt gtgaccgaac atcccgtggt
      661 cgtattcgat caggacggca ccttcctgtc gtcctgggga gcgggaatgt tcaagtttcc
      721 ccacgccatc cgtttcgacg cgcagggctt cgtctgggtt actgacggtc accaccagca
      781 gttcatgaag tttacccccg atggccagtt gctggagacc atcggcatca aggggcagcg
      841 gtcggatacc ggtgttccgg acgacgattt cacctcggtc gcgtggaaga aggtgacgca
      901 cggcggcggg ccgttcaatc ttccaacgga catcgctttc gcccctgacg gatcgatgtt
      961 catgaccgat ggctatggca atgcccgcgt gcataagttc tcggctgacg cgaagcatct
     1021 gttttcgtgg ggagagccgg gtaccgcgcc gggccagttc aatctgcctc atgggatctg
     1081 gatcgaccgg cgcggccggc tgttggtggc cgaccgcgag aacgatcggg tgcaaatctt
     1141 cgaccaggag ggccacctgc tggagatctg gccgacagag ctaatcggcc ctgcgttctt
     1201 ttatgtggac gcggacgaca tcgtttacgt cgccgaacac aacggtggga tgatcagcat
     1261 cctgacgctg gatggcgagc ggctggctcg ctggggcgcc ccgatccatc gctcctgcca
     1321 tgggatctgg ggcgactcgc acggcgatat ctatgttgtg cagccaggcg aatgggggcg
     1381 cgtgcgacgc gtggtgaagt tcgagcggca atagatcgat cgcctgcgcc gggacgttac
     1441 gcgccactag cgtgccgcgc accgaagccg catcgggcaa cccgccccca atcagcgtcc
     1501 cgatcagcgc cccaaagtcg gcatcgtgaa ctccgacccg cgacgggttc cggctgtcca
     1561 acgctgcgtt acggctttgt atcgcgtata gaaccgcaca ccctcgggtc catagatatg
     1621 gtgatcccca aacaacgagt gcttccatcc cccaaacgag tgaaaagcca tcggcactgg
     1681 gatcggcaca ttgatgccta ccatcccgat ctggatcgct tgggtaaagt cgcgagcaac
     1741 gccgccgtcg ttggtgaaca acgcaactcc gttgccatag tcgtgcgcgt tgatcatgcg
     1801 cgtcgccgtt gcgacatccg gcgcccgtac cacggacagc accggcccga agatctcgtc
     1861 cttgtagata cgcatgtccg gcgtcacgtt gtcgaacagg cagccgccga ggaagaatcc
     1921 gttctcgtat ccctgcagct tcaatccgcg gccatcgacg acaaggtcgg cgccctcttg
     1981 cacgcccaga tccacgtacc cccgcacctt ctccaaatgc tcacgggtga ccaacgggcc
     2041 catttcagcc tccggatcga caccgggcgc gaccttcaat gcccgaaccc gcggggcaag
     2101 cttggacatc agcgcatcgc cggcagaccc aacggtcacg gcgacggaaa ctgccatgca
     2161 gcgctctccg gcggcgccat aggccgatcc catcagcgca tcgaccgcat cgtcgatatc
     2221 ggcgtccggc atgacgacca gatggttctt cgccccgccc agcgcctgca cgcgcttgcc
     2281 gtgcgacgtg ccgccgcgat agatcgtctc ggcgatggcg gtagagccga cgaagctgat
     2341 cgcggcgatg ccaggatggg cgatgatcgt ttccaccgcc tcccgcccgc cctgcacgac
     2401 gctgaacaca ccgtccggca gcccggcttc cttcaaaagc tcggctagtt tcaacgcgac
     2461 cgacggatcc ttctcggatg gcttcaggat gaagcagttg ccggtggcga gtgccaccgg
     2521 gatcatccac agcggcacca tgatcgggaa gttgaatggc gttatcccag cgaccacacc
     2581 caggggctgg cggatcgacc atgcatcgat cccggtaccg acctggtcgg tatattcgcc
     2641 cttcagcaaa tgcgggatgc cgcaggcgaa ctcgacgacc tccagcccgc gctgcacctc
     2701 accatccgcg tccgacagca ccttgccatg ctcatcggtg atgatcgcgg acatctcgcg
     2761 gcggtcgcgc tcgatcagtt cctttagctt gaacatcacc cgggcgcggc gcagcggcgg
     2821 cgtgttggcc caggccggcc atacggcgtt cgcggcggcg acagcgacgt ccacctcgga
     2881 ggcattcgcc atcgcgacct tggcggacac ctcgccggag gccggattga acacgtggcc
     2941 aaaactgccg ctggtgccgg caacgtgctt gccgccgatg aagtgcccaa tttcgcgcat
     3001 ggtgtttcct cctgatccgc ctggaactag accagccggg gattgtggtc cagccacagc
     3061 tatggatcga tcacctgaca aaagcgcggc atcccggacg gcagcgacca agctgcctac
     3121 ggcgtgtgcc gcgatcgccg gctgagagca tttgttagcc tgactgtatc agcctgactg
     3181 tgcaggccga aaaatgctct acctctttgt ttaaccgcgc gattctttgt ttgaccgcgt
     3241 gattcacacg cttcggacat tctgcccagc gtgatcaagc tctattttcc gccgcgtcgg
     3301 tcgccaagat gttccagcag cacgaccgcg ccgggcagct cgtgtcgccg ctcccgcagc
     3361 agtttttcgc cgccgacagc gtacaccgcc accgccgaaa gcaggtcgcg cagccgaccg
     3421 ggggcgctgg cgtcgaacgg cagcacacag ccaccggtgc gcttggcaag atcccagaat
     3481 acctcggcat cgcggcgggc cgccggatcg gctgtgtcat gcagcacgat cagcttggtt
     3541 ccctgcaccc ccagggcgtc ggctagtcgg cgaccctgcg acatgctctc ctcgaacacg
     3601 tcgccgatat agatcaccac ccgcaccgcc ggctgcttga ggctggccaa gaggatcggc
     3661 agcagccggg tcaacccggc ctcgcacgcc accccagcgg cacggtcgcg cagcgtgctc
     3721 gcgtgattcg tgaacgcggt gaaggtgtgc acccgcgagc cgccatgcac agcaagcgcc
     3781 acgtccagct cgcccggcag cgccttgacc agcgcatccg tcacctgcct tgccgccgcc
     3841 caagccggct cacgcgaggc ggtggcatcc accgcgaaca ccagacgcgg gcgggccgga
     3901 acaccgctct cggccggagg ttcggcatgg cccagcgcgg aaaggagcga tgccgcggtg
     3961 gacggggcgg ccgccaccgc ggtggtcgcc atagcccgca gacgagccag tatgccgcct
     4021 cgcggagccg gtgtgatggg cggcgcatcg tcggggatca ggacaaaatt ccgcttgtcg
     4081 ctcatggccg cctccgaagc ttggcagcct cttgccgcaa ttggcctctt gctgcaatcg
     4141 gccgggggtt caaccgaacg ctgtttcggt ccctcagccg tcgagcaccc gccgtcgagc
     4201 acccgccccg tcctgcaccc agtgacgcac tgatcgcaga cgccttgcct gggccatcga
     4261 atcgatgatt cggtgccggc atcatctcgg ttcatgccga gaaatcgtgc aatgcctgcg
     4321 gatggacagt cagatccctg cgatacgatg gccacgtttg gtcgaattgc caaagcaaca
     4381 gttcgtaacg actggctgac cctgccaccc acccgtgatg gcaaccgcga cagcctcaaa
     4441 ccgttagcct cgccatgcag tggcccggcc tccgcgactg gatgttcgcg tccaagacct
     4501 tcgtcgcggc ggtgctggcg ctgtacatcg cgctgagcct cggcctggac cggccatact
     4561 gggcgatggc ctccgcctac atcgcctctc agccgctatc cggggccacc cgctccaagg
     4621 ctgtctatcg gctttgcggc acggtggccg gagcgaccgc cgcggtcctc ctggtgcccg
     4681 cattcgccga agcgccgcca ctgctgtcgc tcgttctcgc gttatgggtc gcgggctgcc
     4741 tgtatctttc gctgctggac cgcacccccc gcagctacgc ctgcatgttg gccggctata
     4801 gcgccgccat catcggcttt ccgtcggtcg ttgccccgga agcgatcttc gacaccgcgc
     4861 tgacgcgcgt cgaggaaatt gccatcggcg tttccctcgc caccctggtg gccggcatcg
     4921 tgtttccccg tccggtcggc ccggcgctga tcacccgtat cgacgcctgg ttcgaaaacg
     4981 cccgccgctg gagtatcgag gccctgtccg atcgcgacac cgctgccatg gaagcgcgca
     5041 ggttggccgc cgacggcgtc gaaatcaacc tgctcgcgag ccatctgagc tatgacgcca
     5101 cagcgtggga aactgtgcat ttcggccagt tgcgcgcccg catgatcatg ctgatcccca
     5161 tcctggcctc gattgccgac cgcatggccg cgctacgcgc aagaatgccc cagccggctg
     5221 ccgcgctagc cgcggacctg gtggagtggc tcgaaagcgg ggtatcgggc gacgctcagt
     5281 cattgtcggc gcgcatcgat gaaatggcgg ccagcctgtc ccgcaaatcc gactgggacg
     5341 ctttgctgct gaccagcctg ctgatccgga tgcgcgaact gaccggtatc ctgaccgatt
     5401 gcgcgacctt gcggcggcag atggcatccc cggttcggcc ggcggaggcc gcattgcact
     5461 ttgcccccga ggcagtggcg gctcccgcct ggcatcgcga ccacggcatg gcgttgctgt
     5521 ccgccgcggc ttgtgccgtt gccatcctgc tgagctgcac gatctggatc gccaccgcct
     5581 ggaacgaggg ctacatcgcc gccgagatgg tcgccgtcgc ctgcagcttc ttcgccgcgc
     5641 aggacgaccc ggtgccggcg atcatacagt tcctgggctg gtccctggtt gccgtggtca
     5701 tggacggcat tttgctgttc ggcgtgctgc ccgcggcatc ggatttcatc accgttgttc
     5761 tggcactggc gccgctgttc ttgattggag gcgttttgat cgcgatgcca gccacagcaa
     5821 acggcggccg ggcgttcacg gcgaacgcgg cgaccttgct ggcattgcag agtgcttaca
     5881 ccgccgactt cccggccttc gtcaacagcg gattgggctt cctgctggga cttgcgctgg
     5941 ccgcgaccgt gacgcggctg atccggtccg tcggtgccgg atttggtgtt taccggctgc
     6001 tgcggattat ctggctggat ctcgccatcg ccgcggaacg ccggggcaac cgggatcgcg
     6061 cacgttatgc ggggctgatg ctcgaccgtc tgggcctgat cgcgccccga ctggcggaaa
     6121 ccgaacccga tgccgccccg acagacgcgc tgaccgacat ccgtatcggc ttcaacatca
     6181 tcgacctccg ccgggctcgg cacgtcgtgg cactgccggt acgcgaaagg atcgacgcga
     6241 tgttggatgc cctggccaac gcctaccgct ccaggc
//
LOCUS       DASWQN010000452         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_43787, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000452 DASWQN010000000
VERSION     DASWQN010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43787"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /gene="uvrA"
                     /locus_tag="VGC82_07445"
     CDS             <1..1438
                     /gene="uvrA"
                     /locus_tag="VGC82_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY0423141.1"
                     /translation="LDYLTLARGSASLSGGESQRIRLASQIGSGLTGVLYVLDEPSIG
                     LHQRDNERLLGTLRRLRDLGNTVLVVEHDEDAIRAADHLIDMGPAAGINGGWVIAEGT
                     PNEVAANPKSLTGDYLSGRRMIPVPPMRRPAQKDRMLRVVGARGNNLKNISADFPLGV
                     FTCVTGVSGGGKSTLVIDTLYKAAARRLMNAGTVPTLHDHVEGLDLIDKIIDIDQSPI
                     GRTPRSNPATYTDLFAPIRDWFAELPESRARGYKSGRFSFNVKGGRCEACQGDGLLKI
                     EMHFLPDVYVTCDTCKGKRYNRETLEIKFRDKSIAEVLAMTVDEAVPYFSAQPRIHDR
                     LKILQQVGLGYIALGQQATTLSGGEAQRIKLSKELARRATGRTLYILDEPTTGLHFED
                     VRKLLEVLHTLVDQGNTVVVIEHNLEVIKTADWILDLGPEGGDGGGRIVAEGTPEDIV
                     ANPESHTGHFLAPLLPAIEAPKKTRRRA"
BASE COUNT          325 a          552 c          545 g          265 t
ORIGIN      
        1 actggattac ctgacgctgg cgcgcggctc cgcttcgctg tccggtggcg agtcgcagcg
       61 aatccgcctc gccagccaga tcggttcggg tctgaccggc gtgctgtatg tgctggacga
      121 gccgtcgatc ggcttgcacc agcgcgacaa cgaacgcctg ctgggcacat tgcgccggct
      181 tcgcgacctc gggaacaccg tcctcgtcgt cgaacacgat gaggacgcca tccgagcggc
      241 ggatcatctg atcgacatgg ggccggccgc agggatcaat ggcggctggg tgatcgccga
      301 gggcacgccg aacgaggtcg ccgccaaccc gaaatcgctg accggagact acctgtccgg
      361 acggcgcatg ataccggtgc cgccgatgcg ccgtccggcg cagaaggacc gcatgctgcg
      421 ggtcgttggc gcacgcggca acaacctgaa gaacatctcg gcggatttcc cgctgggtgt
      481 atttacatgt gtcaccggcg tctccggcgg cggcaaatcc acgctggtga tcgacacgct
      541 gtacaaagcc gccgcgcgcc gcctgatgaa tgccgggacc gtgccgacgc tgcatgacca
      601 tgtcgagggc ctggacctga tcgacaagat catcgacatc gaccagtcgc cgatcggccg
      661 cacgccccga tccaatccgg cgacctatac cgacctgttc gcgccgatcc gcgactggtt
      721 cgccgaactg ccggaatcga gggcgcgcgg ctacaagtcc ggccgtttca gcttcaacgt
      781 caaaggcggc cgctgcgagg cctgccaggg tgacggcctg ctgaagatcg agatgcactt
      841 cctgccggac gtctatgtca cctgcgacac ctgcaagggc aaacgctaca accgcgaaac
      901 gctggagata aaattccgcg acaagtcgat cgccgaagtg ctggcgatga cggtggacga
      961 ggcggtgccc tatttcagcg cccagccccg catccacgac cggctgaaaa tcctccagca
     1021 ggtcggcctg ggatacatcg cgctcggcca gcaggccacg acgctgtccg gcggcgaggc
     1081 gcagcggatc aagctgtcca aggaactcgc ccggcgcgcc accggccgca cgctgtacat
     1141 cctggatgaa cccaccactg ggctgcactt cgaggatgtc cgcaaactgc tggaagtgct
     1201 gcacacgctg gtcgatcagg gcaacacggt ggtggtgatc gagcacaacc tggaggtcat
     1261 caagaccgcc gactggatcc tggacctagg ccccgaaggt ggcgacggcg gcggccggat
     1321 cgtcgccgag ggcacgccgg aggacatcgt ggcgaacccc gagagccaca ccgggcactt
     1381 cctcgccccg ttgctgccgg cgatcgaggc gccaaagaag acaaggcggc gggcgtagag
     1441 cggcttcgcg ccaaccaacc ccgtggtgtc cgcccaaacc cgcatgggcg gcgtggaagg
     1501 tgcgcgtgtc gggttcgaac gggcgcccgg gggcaactca tgaatgagcc gagcgttatt
     1561 acgtgcgtcc gagaactcaa aggtggggtt caccatgaag ctgatttcaa cgcgcgcaaa
     1621 actgtcacgt gcttcgctga gccttattgg cgtcctggct ttcacgctcc cgagcctggc
     1681 acaaaat
//
LOCUS       DASWQN010000453         3875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2154512, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000453 DASWQN010000000
VERSION     DASWQN010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2154512"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1446
                     /locus_tag="VGC82_07450"
     CDS             169..1446
                     /locus_tag="VGC82_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0423142.1"
                     /translation="MRRRDLLRAVSAYAAAPLAAPALAQSQKPLRFIPQANLTVLDPI
                     WTTAVITYNHAYMVYDQLYGWDGARIRPQMAEGHDISQDELTWTFTLRDGLKFHDNEP
                     VLARDAAQSIRRWGSRDSFGQVLMRYVNEIVALDDKRFAIRLRKRCPQLLFGFGARQS
                     FIMPERLAIKAGTEQVTDPVGSGPFRFVKDEWVSGVSCTYAKFDGYVPRNEPPAFFAG
                     GKVAHFDRVEWTVQPDPATAAAALQKNEVDWLEQPLIDLVPMLNKSPGVYTKVVDPGG
                     WLMFMVINHLNPPFDNPKIRHAIIVALDQQAFVDSVVGDQHELSIVPTGLFAPTMPMS
                     NNAGLERLTGPRDIAKAKQMIAESGYKGEPVMIMSPSDQPALKQMSEVAREFFESIGL
                     KVDLQTMDWGSVITRRANQKPLDQGGGERSSRS"
     gene            1443..1739
                     /locus_tag="VGC82_07455"
     CDS             1443..1739
                     /locus_tag="VGC82_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423143.1"
                     /translation="MSPLTSANPGSMLSLRGNGRNGWFGWPTDPRIEELREAWFDAPD
                     LAAQQAVAQQIQLQYFQSVPFLALCRMRQPMAFRSDIRDVVSASFPVFWGVRRG"
     gene            1761..2525
                     /locus_tag="VGC82_07460"
     CDS             1761..2525
                     /locus_tag="VGC82_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423144.1"
                     /translation="MTDWTETYRGAVAPWQCDATEHFTIAYYFDRLEQAEANLADELG
                     LPNLPRRGDLPRRINARFARELHAGASFHVESAALSVDNGLRLGHRFVDSANGEVVTW
                     FDEHWDLSAAPLTSQQRRAVEARLAVWNGPAVEPRPEPATTNGFIPTARGRVKPGDVD
                     AAGHFALEAIMHRFSNASGQLGALIGMDNALQQRRGFSTFELILLMSEVLRLDAPYLV
                     ETGVGHLGSSSLRMIHRMTDPRTGVEVAAQPVRRQS"
     gene            2993..3343
                     /locus_tag="VGC82_07465"
     CDS             2993..3343
                     /locus_tag="VGC82_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423145.1"
                     /translation="MPISTRYVFIVSMDVSPEKEALFNEVYEQEHVPNLLKVPGVRGV
                     TRLRTECANLTLGGQLKELTGEGAPRYVAIYEIDSPDVLTSAAWAEAAEDGRWPAEVR
                     PYTSNRHHIVRAVI"
     gene            3616..>3875
                     /locus_tag="VGC82_07470"
     CDS             3616..>3875
                     /locus_tag="VGC82_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0423146.1"
                     /translation="MMTARNDVELALVERARGLLPAGGLGNLASDTIIARGRAGRVWD
                     VSGREYVDLLLGSGPMLVGHAHPEVTQAVLEQVPLGTTFFTN"
BASE COUNT          740 a         1234 c         1190 g          711 t
ORIGIN      
        1 ccgggacgta gtgccacagt tgccttcagc cggggggtca tcatcggtgc cggacaaaaa
       61 cgccgatgca ggccaaactg gcgcctgagg cctgttatcc ggtgagttgc aacctggctc
      121 ggttcagtcc acatcacagc caacaaaaat gtaccgggag gcccctccat gcgccgtcgt
      181 gaccttctga gagccgtgtc tgcgtatgcc gccgcgcccc tcgccgcccc cgccttggcg
      241 cagtcgcaaa agccgcttcg cttcattccg caggccaacc tgaccgtgct cgacccgatc
      301 tggacgacgg ccgtcatcac ctacaatcac gcgtatatgg tctacgacca gctatacggc
      361 tgggatggcg cacgaatccg cccgcagatg gccgaaggac acgacatctc ccaggatgag
      421 ctcacctgga ccttcacttt gcgagacggg ttgaaattcc acgacaacga acccgtcctc
      481 gctcgagatg ctgcgcaatc gatccgccgc tggggatcgc gcgattcgtt cggccaggtc
      541 ctgatgcgct acgtgaacga aatcgtggcg ctggacgaca agcgcttcgc cattcgcctc
      601 aggaagcgct gcccccagct gctgttcggg ttcggtgcgc gtcaaagctt tatcatgccc
      661 gaacgcctcg ccatcaaagc aggcaccgag caggtgacgg accccgtcgg ttcggggcca
      721 tttcggtttg taaaggatga atgggtgtcc ggcgtcagtt gcacctatgc caaattcgac
      781 ggctatgtgc cgcggaatga accgcccgca ttcttcgccg gcggcaaggt ggcgcatttc
      841 gatcgggtgg aatggacggt gcagccagat cccgccaccg ccgcggccgc cctgcagaag
      901 aacgaggtcg attggctgga acagccactg atcgatctgg tcccgatgct caacaaatcc
      961 ccgggtgtct ataccaaggt cgtcgatccc ggcggctggc tgatgttcat ggtgatcaac
     1021 cacctgaacc cgccgttcga caaccccaaa atccgtcacg ccatcatcgt cgccctcgat
     1081 cagcaggctt tcgtcgattc cgtggtgggg gaccagcacg agctttccat cgtcccgacc
     1141 ggtcttttcg ccccgaccat gccgatgtcc aataacgccg ggctggaacg gctgaccggc
     1201 ccgcgcgaca tcgccaaggc caagcagatg atcgccgagt ccggctacaa gggcgagcca
     1261 gtgatgataa tgtcccccag cgaccagccg gctctgaaac agatgtcgga ggtcgcgcgc
     1321 gagttcttcg agtccatcgg tctgaaagtc gatctgcaga ccatggactg gggcagcgtg
     1381 ataacccgtc gagccaatca gaagccgctg gaccaggggg gtggggagcg ttcatcacgg
     1441 tcatgagccc gctgacctcc gccaaccccg gcagcatgct gtcgttgcgc ggcaacggcc
     1501 gcaatggttg gttcggctgg cccaccgacc cgcggatcga agagctccgc gaagcctggt
     1561 ttgacgcgcc cgacctagcc gcgcagcagg cggtggccca acagatccag ttgcagtatt
     1621 tccaaagcgt gccgttcctg gcgctgtgcc ggatgcgtca gccaatggcg tttcgctccg
     1681 acatacggga cgtcgtctcg gcctccttcc ccgtgttctg gggcgtgcgc cgagggtaac
     1741 tactttggcg gagacgacgg atgactgatt ggaccgagac ctatcgaggc gctgtggcgc
     1801 cctggcaatg cgacgcgacc gagcatttca cgatcgccta ttacttcgat cggctggaac
     1861 aggcggaagc caatctcgcc gacgaacttg gccttcctaa cctgccgcgc cgcggagacc
     1921 tgccccgccg gatcaacgcc cgcttcgcac gcgagctgca cgccggcgcc agctttcatg
     1981 tcgagagcgc ggcgctaagt gtcgataacg ggttgcgcct gggccatcgt tttgttgatt
     2041 cggccaacgg cgaagtcgtg acctggttcg acgagcactg ggacctctcc gccgcgccac
     2101 tgacatccca acaacgccgc gcggtcgaag cccgactcgc ggtctggaat ggtccggcgg
     2161 tcgaaccgcg acccgaaccg gccacgacaa acggtttcat ccccaccgca cgcggacggg
     2221 ttaaaccagg tgatgtcgac gcagccggcc atttcgctct ggaggcgatc atgcaccgct
     2281 tttccaacgc ctccggccag cttggcgctc tgatcggcat ggataacgct ttgcagcagc
     2341 gacggggctt ttcaaccttc gagctgatct tgcttatgtc cgaagtactg cggctggacg
     2401 cgccatacct ggtcgaaacc ggcgtcggtc atctcggcag ttcttcactg cgcatgatcc
     2461 atcgcatgac cgacccgcgg acgggcgtcg aagtcgcggc tcagccagtt cggcgtcaat
     2521 cttgatctcg acgcgcgccg tcccgcgcgc tggtctgacg ccatccgcca ccgggccgtc
     2581 gcgctggtcg tgccggccag ctaaacttct gctttcctct gcctgccact cctttgcagc
     2641 tcggaacgtt gctgggcggg aggtgtgctt tggagcagca gcgctgctcc attctacttg
     2701 gacaactgga tgccaactat gggcgtgaag tggccaaagc agagcggcca ctttggagtg
     2761 tccaacgggg acgagcggac gctcgttctt acaaatcgtc gacgataggt cgcgatccaa
     2821 ggacatactc gcgctcgtac aaaagctcgc cctgcgacac gagatccgct cggactcatc
     2881 gccgctcatc ctccgatcat ggggtgtcaa ccggcgccct ttggcggcaa actgacccaa
     2941 ggacagacag cgaccgctga ccgaacatga gtaatagcca caaggatcag aaatgccgat
     3001 ctcgacgcgt tacgtcttta tcgtgagcat ggatgtaagc ccggaaaagg aggccctctt
     3061 caacgaggta tacgagcagg agcacgtgcc gaacctgctg aaggtcccgg gcgttcgtgg
     3121 cgttactcgg ttacgaaccg agtgcgcgaa tcttaccctc ggcggtcaac tgaaggaatt
     3181 gaccggtgaa ggggcgccaa gatacgtcgc gatttatgag atcgacagcc cggacgttct
     3241 gacgagcgct gcatgggcgg aagcagcgga ggacggccgc tggccggccg aggtccggcc
     3301 ctacacgagc aaccggcacc acatcgtccg cgctgtgatt taggctgtct atctgcttct
     3361 cgcgttgatc gagtgtgtgg ccgcgaccgc ggtggaagcc acacgacggt gcctgaccag
     3421 aaggagccaa tgccgtgcgg cggatgcctc ctgaacgcgc ctgatcggtc gagcgtgccc
     3481 gacaggatgc cctatcatct gaatcgtgga ctggaggcgg gggcaaccca ggtttctcgt
     3541 tgacaggagt ggtagtcaca cttgggcgag acagcccact cgcctgaaga tactgacata
     3601 ggaagggcga cacgcatgat gacagcacga aacgacgtcg agttggcgct ggtggagcgg
     3661 gcgcgcggac tgctgccggc cggcgggctc ggcaaccttg cctccgacac catcatcgcc
     3721 aggggccgcg ccggccgggt gtgggatgtc agcgggcgcg aatacgtcga cctcctgctg
     3781 gggtcgggac cgatgttggt gggtcatgcc catccggagg tgacgcaggc ggtgctggaa
     3841 caagtgccgc tcggcacgac cttcttcacc aacaa
//
LOCUS       DASWQN010000454         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1759042, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000454 DASWQN010000000
VERSION     DASWQN010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759042"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1217)
                     /locus_tag="VGC82_07475"
     CDS             complement(78..1217)
                     /locus_tag="VGC82_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEY0423147.1"
                     /translation="MSDKKPVPFLDLKAQQARIAGDLRARLDKVLEHCQFVLGPEVRE
                     LESALANFCGAAHCVSVSSGTDALQIAMMAEGIGTGDAVFLPAFTYTATAEVPLLLGA
                     TPIFVDVDPRTFQIDPGHLTQRIQDVRTAGRLKPRAIIGVDLFGQPADWPCLLDIARL
                     HDLFLLDDLAQSFGASLHGKMLGTQADATATSFFPSKPLGAYGDGGALFTESEDRADL
                     YRSLRTHGEGTTRYEVLRTGVNGRLDSMQAAVLLAKLAVFPEELQARERIARVYDQRL
                     GNAVMTPKRIPDSTSAWAIYAILLPDGAMRERTQAAMRAEGVPTAIYYPCPLHRQPAY
                     STQHDGASLPVSEDLATRILALPIHPDLTDEDVDRVCEAVLAAVR"
     gene            complement(1340..>2594)
                     /locus_tag="VGC82_07480"
     CDS             complement(1340..>2594)
                     /locus_tag="VGC82_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEY0423148.1"
                     /translation="GTRLAQVVAQADRFGLQVRQAPRPTALADPDKPRPVELRPVAIE
                     DLLNRPQVPLDRDGMARLIQGRRVIVTGAGGTIGSELARQVAAFGPGSLILLDNGEYA
                     LWQIDLELAETHPQVNRRTLIADIRDEGRVRAIFDEHRPELVFHAAALKHVPMVEANP
                     LEGLATNAAGTRYVADAARATGALAMVLISTDKAVNPTSVMGASKRLAEMYCQALDIS
                     ARSSRHGMRCITVRFGNVLGSTGSVVPLFQRQLARGGPLTVTHPDMQRYFMTVREAVG
                     LVLQASVLVVGGASLPSDKNGGIFVLDMGEPVKIVDLARQMIRLVGLQPEQDIEIRFT
                     GLRPGEKLYEELFHGKEPPVPTGYAGLLMASPRTADPAIVARAIEEIGNACRGGQVKL
                     ALTLLGRQIPEFEHNIDGSAGEVRA"
BASE COUNT          456 a          843 c          850 g          445 t
ORIGIN      
        1 gcggtccgtg atcatgccgt ttgcacttaa actcgagcag aacgcccccg ggcgcgcgac
       61 agaggtcccg cggaccgcta cctaacggca gccaacaccg cctcgcagac tcggtcaaca
      121 tcctcgtccg tcaggtccgg atggatcggg agcgcgagga tacgggtcgc cagatcctcc
      181 gataccggca gtgatgcgcc gtcatgctgc gtgctgtatg ccggctggcg gtgtagcggg
      241 caggggtagt agatcgcggt cggcacgcct tcggcccgca tcgccgcctg ggtgcgttcc
      301 cgcatggccc cgtccggtag caggatcgcg tagattgccc acgcgctggt gctgtccggg
      361 attcgcttcg gcgtcatcac cgcgttaccg agccgctggt cgtaaactcg ggcaatgcgc
      421 tcgcgggcct gaagttcttc gggaaagacc gcgagtttcg ccagcagcac cgcagcctgc
      481 attgaatcca ggcgcccgtt caccccggtg cgcaaaactt catagcgcgt cgtaccctcg
      541 ccatgcgttc gcaggctgcg ataaaggtcc gcgcgatctt cgctttcggt gaaaagagca
      601 ccaccgtctc cgtatgcacc cagcggcttg gacggaaaga agctcgtcgc ggtcgcgtcg
      661 gcctgggtgc caagcatctt gccgtgcagc gaggcgccga agctttgggc aaggtcgtcg
      721 agcagaaaca ggtcgtgcag gcgcgcgata tcgagaaggc aaggccaatc ggcaggctgg
      781 ccaaaaagat cgacaccgat gatcgcgcgt ggcttcagcc ttccggctgt ccggacgtcc
      841 tgaatccgtt gcgtcagatg gccgggatcg atctggaagg ttcggggatc gacatcgaca
      901 aagatggggg tggcgccgag cagcagcgga acctcggctg tcgcggtgta ggtgaaggcg
      961 ggcagaaaga cggcgtcgcc cgtgccgatt ccctcggcca tcatggcgat ctgcaaggca
     1021 tcagtgccgg agctgacgct gacgcagtgc gccgccccgc agaagtttgc cagcgcggat
     1081 tccagctcac gcacctcggg gccgaggacg aattggcagt gctccagcac cttgtccaag
     1141 cgggcacgca gatcgcccgc gatccgggcc tgctgggcct taaggtcaag gaacggcacg
     1201 ggcttcttgt cagacatggt ttaactcttc gtttgccggc atgatttacc cgccatcggc
     1261 gttccgcctg ccgcgatcat gctcggtatc tgctcgcatg attcacccgc catcggcgtt
     1321 ccgcctgccg cgatcatgct caggctcgga cttcgccggc ggagccgtcg atgttatgct
     1381 cgaactccgg aatctggcgg cccagcagcg taagcgccag cttgacctgg ccaccccggc
     1441 aggcattgcc gatttcctcg atcgcgcggg caacgattgc cggatcggcg gtccggggcg
     1501 acgccatcag cagaccggca tagcccgttg gaaccggtgg ttccttgccg tggaacagct
     1561 cctcatacag cttctccccc gggcgaaggc cggtgaagcg gatttcgatg tcctgctcgg
     1621 gctgcaagcc aaccaaacgg atcatctgcc gagcgagatc gacgatcttc accggctctc
     1681 ccatgtcgag gacgaagatg ccgccattct tgtcggacgg cagcgatgca ccccccacca
     1741 ccagcacgct ggcctgcagg accaagccga ccgcttcgcg caccgtcatg aaatagcgct
     1801 gcatatccgg gtgggtgacc gtcaatggac cgccgcgtgc gagttgacgc tgaaacagcg
     1861 gcacaaccga accggtggag ccaagcacgt tgccgaagcg gacggtgatg cagcgcattc
     1921 cgtggcggct ggagcgcgcc gagatatcaa gcgcctggca gtacatctcg gccaggcgct
     1981 tggaggcgcc catgacgctg gtcgggttca ccgccttgtc ggtggaaatc agcaccatcg
     2041 ccaaagcacc ggtggcccgg gcagcatcgg cgacgtagcg agtgcccgcg gcgttggtgg
     2101 ccaggccctc caacgggttg gcctcgacca tcggcacatg tttcagcgcc gccgcgtgaa
     2161 acaccagttc cggccggtgc tcgtcgaaga ttgcccgcac gcggccttcg tcgcggatat
     2221 cggcgataag cgtgcggcgg ttcacctggg ggtgggtttc cgccagctcc agatcgatct
     2281 gccatagggc gtattcgcca ttgtccagca ggatcaggga gccggggcca aatgcagcca
     2341 cctggcgagc caattcgctg ccgatcgtgc cgccggcccc agtgacgatg acccggcggc
     2401 cctggatcag ccgggccatt ccgtcccggt cgagcggcac ctgcgggcga ttcagcaggt
     2461 cctcgatcgc gaccggacgt agttcgaccg gacgcggctt gtccggatcg gcaagcgcgg
     2521 tgggccgagg cgcctggcgc acctgaagcc cgaagcggtc cgcctgagca acgacctggg
     2581 caaggcgggt gccg
//
LOCUS       DASWQN010000455         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_835610, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000455 DASWQN010000000
VERSION     DASWQN010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835610"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..741)
                     /locus_tag="VGC82_07485"
     CDS             complement(286..741)
                     /locus_tag="VGC82_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEY0423149.1"
                     /translation="MIHYQLRCRQSHGFDGWFNDSASFEKQAKRGLIECPECGDTSVE
                     RALMAPAVAKRESLPVPVAAPQPPAPTEKAAMPVPAQMLAMLQRMRAEVEKNCDYVGP
                     DFADRARAMHRGEAEAKPIYGETSEEQAESLADEGIQVAKIPWVPRADG"
     gene            complement(809..1069)
                     /gene="grxC"
                     /locus_tag="VGC82_07490"
     CDS             complement(809..1069)
                     /gene="grxC"
                     /locus_tag="VGC82_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY0423150.1"
                     /translation="MPNVEIYTQSFCPYCERAVALLSKKGVAFNEIDAPYGSKAREAA
                     RERSGGRTSAPQIFIAGQHIGGCDELMALDRAGKLDAMLGLA"
     gene            complement(1075..2226)
                     /locus_tag="VGC82_07495"
     CDS             complement(1075..2226)
                     /locus_tag="VGC82_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEY0423151.1"
                     /translation="MTQAAFLRDPALGRVWDALPDARVVGGAVRDALAERPVADIDLA
                     TPSSPADVTEALKQAAIKVVPTGLAHGTVTAVVAGRGFEVTTLRRDITTDGRHAVVAF
                     TADWRLDASRRDFTINAMSMARDGTVFDYFDGQADLHAGRVRFVGDPATRIAEDYLRV
                     LRFFRFYARYARVPPDPATLDALRAGASGLPRLSVERVWTELRRILEAPEPTPAILLM
                     HLLGIWEAVLPEAPAIDRMAGLPADPVLRLAAMLTGDPLALAARLKLSNGDRDRLVRL
                     LATPHPSGTDADLRRLLADHLPQDLIDRTWLDGAADVRRRLTGMKRPVFPLEGRDVVA
                     AGIPQGPRVGALLRAVRQWWLDGGCVADHAACLANLAPLADPSKIGGKR"
     gene            complement(2223..2471)
                     /locus_tag="VGC82_07500"
     CDS             complement(2223..2471)
                     /locus_tag="VGC82_07500"
                     /inference="COORDINATES: protein motif:HMM:NF040327.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HEY0423152.1"
                     /translation="MSRIIEIMLFLAPFLCFAVWRLLFPSPLPPLWLVGGLTGFVVLM
                     LIGLVWLRHLDAGDANQGYVPARLLDGRVVPPQRTETP"
     gene            complement(2596..3486)
                     /gene="rfbD"
                     /locus_tag="VGC82_07505"
     CDS             complement(2596..3486)
                     /gene="rfbD"
                     /locus_tag="VGC82_07505"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEY0423153.1"
                     /translation="MTPPRAILVTGGTGQLASALANHAGILRVGRPDFDFDHPNTIEA
                     AFQAAAPRVVVNAAAYTAVDAAESNADAAYRTNRDGPAILAALCTAANIPLIHVSTDY
                     VFDGAKPEPYVETDPVAPQGVYGASKLAGEEAVTASAANAIILRTAWVYAATGKNFVR
                     TMLAAGKTRDRLTVVADQHGCPTTATDLSDAILAIVARIDRTGWQPAYGGVFHAAGAG
                     ATTWHGLAVATFEEAARHGAKMPEIVPITTADWPTPAKRPANSRLDCTRLHQVFGIRL
                     PHWRESLTRTVDTIFATTPP"
BASE COUNT          615 a         1149 c         1203 g          587 t
ORIGIN      
        1 agaacagata gggttgcact tgcatggcgt ttccttgcgg tggcgacttt gatacgtccg
       61 aacgacgtat ctagagcatt gcggggctgg cgggaagtgg gatcggcaac gctcgctgaa
      121 tgccgcgttt ggcgccaccg caccgccgcg agcccggcgg cccatccggt cgatcatccg
      181 cccgcgtccg atccagtgcc gcgagcgttg cctcgtccac gaagcgcggt cccaggctgg
      241 aacagcgcga gcgccggctt gaccggcaga taccagccac cgcgatcatc cgtcggcgcg
      301 cggcacccat gggattttag cgacttgaat tccttcgtcg gcgaggcttt ccgcttgttc
      361 ctcggacgtt tcgccgtaga tcggcttggc ctcggcctca ccgcgatgca tcgctcgggc
      421 tcggtcggcg aagtctggcc cgacgtaatc gcagtttttc tccacctcgg cgcgcatgcg
      481 ctgcagcatc gccagcatct gggcgggcac cggcatggcg gctttctcgg tcggggcagg
      541 cggttgcggc gcggcgacag gaaccggcaa ggactcccgc ttggccacgg ccggcgccat
      601 caacgcccgt tcgacgctgg tgtcgccgca ttccgggcac tcgatcaagc cacgcttcgc
      661 ctgcttttca aagctggcgc tatcattgaa ccagccatcg aacccatgag attgtctgca
      721 gcgcaactgg tagtggatca ttccccatcc tgaccgaaac aaattcgtaa cgtaatctgg
      781 cacctgtcac ggcaggatcc taacacaatc atgccaaacc gagcatcgca tccaattttc
      841 cggctctatc caaagccata agctcatcgc aaccgccgat atgttgcccc gcgatgaata
      901 tttgtggcgc cgaggtgcgc ccgccggacc gttcccgcgc tgcctcccgc gccttcgagc
      961 cgtacggagc atcgatctcg ttgaacgcga cgcccttctt tgatagcagc gcgacggccc
     1021 gctcacagta cgggcagaac gattgggtat aaatttcgac atttggcatg tgattcacct
     1081 cttgccgcct attttggacg gatcggccag tggtgcaagg ttcgccaggc acgcggcgtg
     1141 gtccgccacg cagccgccat ccagccacca ctgacgcacc gcgcgcagca gcgccccgac
     1201 tctcggtccc tgcggaatgc cggccgccac cacatcgcgg ccctccaggg ggaaaaccgg
     1261 ccgcttcatg ccagtcagcc gccgccgcac atccgccgca ccgtccagcc acgtccggtc
     1321 gatcaggtcc tgtggcaaat gatccgcgag cagccgccgc agatccgcat cagttccact
     1381 cggatgaggt gtggccagca ggcgaacaag ccggtcgcgg tcaccattcg atagtttcag
     1441 gcgtgccgca agagccagcg gatctccggt cagcatcgcg gcgagccgca acaccgggtc
     1501 ggccggcagc ccggccatcc gatcaatcgc cggtgcctcc ggcagcacgg cttcccagat
     1561 gccgagaagg tgcatcagca agatggccgg ggtcggttcc ggtgcttcga ggatgcggcg
     1621 aagttccgtc cacacccgct ccaccgacag ccgaggcaag ccggaggcgc cggcccgcag
     1681 ggcatccaac gtcgccggat cgggcggcac gcgggcgtag cgagcgtaga agcggaagaa
     1741 gcgaaggacg cggaggtaat cctcggcgat gcgggttgcc ggatcaccga cgaagcggac
     1801 gcggccggca tgaaggtcag cctggccgtc gaagtagtcg aacaccgttc cgtcgcgggc
     1861 catcgacatc gcgttgatgg tgaaatcccg ccgcgaggca tccaggcgcc aatcggcggt
     1921 aaacgcgacc acggcatggc ggccatcggt ggtgatatcg cggcgcaggg tggtgacttc
     1981 gaagccgcgg ccggctacga ctgcggtgac ggtgccatgg gccaggccgg tcggtacgac
     2041 cttgatcgcg gcctgcttca gcgcctccgt cacatccgcg ggcgatgacg gcgtggccag
     2101 atcgatgtcc gccaccggcc gttcggcgag cgcatcgcgt accgcaccgc cgacgacgcg
     2161 ggcatcaggc aatgcgtccc agacgcggcc cagtgccgga tcgcgcagaa acgcggcctg
     2221 ggtcacggcg tctctgtccg ctgcgggggc acgacacggc cgtccaacaa ccgcgcggga
     2281 acgtagccct gattcgcgtc gccggcatcc aggtggcgga gccagaccag accgatcaac
     2341 atgagcacga cgaagccggt cagtccgcct accagccaca acggcggcag cggcgagggg
     2401 aacagcaggc gccagaccgc gaagcaaagg aatggcgcca ggaagagcat gatctcgatg
     2461 atacgcgaca tagccggtcg tccttggcaa agcggcgtcg cggcaagtgg gtcggccgcg
     2521 acggatcggc aacgcgttac gatgttgtcc tggattcagg tcatgctcaa ttgaccggct
     2581 tacggcgggc cgcggttacg gcggcgtcgt ggcgaagatc gtatcgacgg tccgggtcag
     2641 gctttcgcgc cagtgcggca aacggatgcc gaacacctgg tgcaaccggg tgcagtccag
     2701 ccgggagttc gccggacgtt ttgccggcgt cggccagtca gcggtggtga tcggcacgat
     2761 ttcgggcatt ttcgcaccgt ggcgggcagc ttcctcgaac gtggcgacgg cgaggccatg
     2821 ccaggtcgtc gccccggcgc cggcagcgtg gaagacgccg ccatacgccg gctgccagcc
     2881 ggtccgatcg atgcgcgcga cgatcgccag gatggcgtcg ctcagatctg tcgcggtggt
     2941 cgggcagcca tgctgatcgg cgacgaccgt caaccggtcc cgggttttgc cggccgcgag
     3001 catggtgcgg acgaaattct tgccggtggc cgcatacacc caggcggtgc gcaggatgat
     3061 cgcgttggcg gcggatgccg tcacggcttc ctcgccggcg agtttgctgg cgccgtacac
     3121 gccctgtggc gcgacggggt cggtttcgac ataaggttcc ggcttggcac cgtcgaagac
     3181 gtagtcggtg gaaacgtgga tcaggggaat attggccgcg gtgcacaatg cggccagaat
     3241 cgccggtccg tcgcgattgg ttcggtatgc ggcgtcagcg ttgctctcgg ccgcatcgac
     3301 ggcggtatag gcggcggcgt tgacgactac tcgcggggcg gcggcttgga aggcggcctc
     3361 aatggtgttg gggtggtcga agtcgaagtc ggggcggccg acgcggagga ttccggcgtg
     3421 attcgccagg gccgaggcaa gttggccggt gccgccggtg accaggatgg cgcggggggg
     3481 cgtcatggcc gtgttgctcc gtctgccggc aaagtcgtgg aaggctgcgg caagcccggc
     3541 catgacacaa gcgg
//
LOCUS       DASWQN010000456         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1759093, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000456 DASWQN010000000
VERSION     DASWQN010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759093"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VGC82_07510"
     CDS             <1..936
                     /locus_tag="VGC82_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY0423154.1"
                     /translation="TDDAYTEGNAVAVAAKVSGYVTELRVDDNTFVHQGDLLLKIDPR
                     DYLTAREQARASLSLARAELSSAQVDLEVTRTRAPATLLQAQAQLAQAQANQSQAQRE
                     FNRQRAVDPRATTQVSVDQATAQLQANAALVKSAEAQVQIASLVRQTIQAAEDTVAQR
                     QAQVAQADANLAQSEVNLSYAELKAPQDGRVTRRNVDLGTFVQAGQQVFYIVTPRVWV
                     TANFKETQLADIRPGQPVSMAVDAYPNLRLHGHVDSIQEGSGARFSAFPAENATGNFV
                     KIVRRVPVKIIIDSGLDNQGGLPLGISVSPTVTVR"
     gene            988..2558
                     /locus_tag="VGC82_07515"
                     /pseudo
     CDS             988..2558
                     /locus_tag="VGC82_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
     gene            2563..>3231
                     /locus_tag="VGC82_07520"
     CDS             2563..>3231
                     /locus_tag="VGC82_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0423155.1"
                     /translation="MRGIATGALVASLGLFLGACSVGPDFTSPKPPDAAKWNDPSASG
                     SVSTVSTQANPDPQWWGGFGDATLTALIEKAIAGNLDLQQAVLRVVEAQQGEVTARAA
                     GLPSVNGNASYMREQLGLRGLLLSRGINGQINGLAAPNSTLNQFSPGLGNSASTAANG
                     VLNQLSQPVDLFQYGLSSSWELDLFGRVRRSREQAKASTEAQMEATNDALVMLEGQVA
                     QAYVQ"
BASE COUNT          601 a         1028 c          977 g          625 t
ORIGIN      
        1 accgacgatg cctataccga aggcaacgcc gtcgccgtcg ccgccaaagt gtcaggatac
       61 gtcaccgaac tgcgggttga cgacaacacc tttgtgcacc agggcgatct gctgctgaag
      121 atcgacccgc gcgattacct caccgcacgc gaacaggctc gtgccagctt gtccctcgcc
      181 cgcgcagaac tttccagtgc ccaggtcgac ctggaagtta cccgtactcg cgcacccgcg
      241 acgttgctgc aggcacaggc acaactcgcg caggcgcagg cgaatcagag tcaggcgcag
      301 cgtgagttca accggcaacg cgccgtcgat ccgcgtgcaa ccacccaggt cagcgttgac
      361 caggccaccg cgcaactcca agccaatgcc gccttggtga aatcagcgga agcgcaggtg
      421 cagatcgcat ccctggtacg gcagacgatc caggccgccg aggacaccgt cgcgcaacga
      481 caggcccagg tcgcgcaagc cgatgccaac ctcgcgcagt ccgaggtcaa cctgtcctac
      541 gccgaactga aagcacccca ggacggcagg gttacgcggc gcaacgtcga tctggggacc
      601 tttgtccagg caggacagca ggtgttctac atcgtcacgc cgcgggtgtg ggtaacagca
      661 aacttcaagg aaacccagct tgccgacatt cggcccgggc agcctgtgtc gatggcggtc
      721 gatgcctatc ccaacttgcg gctgcacggc catgtggaca gcatccagga aggcagcggg
      781 gcccgctttt cggcgtttcc cgccgagaac gccaccggga atttcgtgaa gatcgttcgc
      841 cgcgtgccgg tcaagatcat catcgacagc gggctggaca accagggcgg gctaccgctg
      901 ggcatttccg tgagccccac ggtcaccgta cgatagggct tggacgataa ggctggacga
      961 tagggcttgg acgatgagat tgggacgatg agcggcttgg agcagagatg gaagccgaag
     1021 tccaacccat ggctggttgc gatcatcgta accctgggta cgttcatgga ggtactcgac
     1081 accacgatcg tgaacgtttc gctgccgcac atcgccggca gcctgtcgat cagcagcgac
     1141 gaaagcacct gggcgctgac gacctacctt gtcgccaacg gcatcgtttt gaccatctcc
     1201 ggcgcactca gccgacagct tggacgcaag aactacttcc tgatctgcat cggcgcgttc
     1261 accctggcct cgcttgcctg cggccttacc gagcagttct ggcagatcct gttgttccgt
     1321 gcgctgcagg gatttttcgg cggcggattg cagccgacgc agcaggcgat cattctcgat
     1381 cacttcccgc cggaaaagcg ccagcaggcg ttttccctga ccgcggtcgc catcatcatc
     1441 gcccctgtgg tcggaccggt cgtcggcggc tggctgaccg acacctatag ctggcactgg
     1501 atcttcctga tcaatatccc gatcgggata ctgacgtttt tcggcgtgct gcaatacgtc
     1561 gaggactccc cgatgataaa gcgtgagaaa aagaacgcac cccgcttcga ttacatcggc
     1621 gtactgttta tcgcggtcgc cctcggctgt cttgaagtcg gtgtggaccg tggcgaagac
     1681 tacgattggc ttagctcgaa ttttatccga ttgatgttct tgttgtccgc gtgcgggttc
     1741 atcttcggca ttacctacct gctttatgtc cgcgacccga tcgtcaacct gcgagtcttc
     1801 aaggaccgca atctcgcgct cggttcgttg cagatcgccc ttatgggctt cgtcctttac
     1861 gccagcgcgg tcctgatccc acagttcgcc cagcagcagc tggactacaa cgcgacctgg
     1921 gccggactgg tgctggcgcc cggcgcggtc gtgctggtca tgctgatccc ggtcgccggc
     1981 aggatcatga acctggtgcc gagaaatacg tgatcgccgg cggtgggctg gcactgggat
     2041 tggccttgat ctactcgatg aatctggttc cgcaactgga cttttggcac ctggtcctgt
     2101 ttcgcgcagc gcaaacggcg gcacttgccc tgttgttcgt gccgatcagc acgatcgcct
     2161 atgcgacgat cccgcccaac ctgaacggtg acgccactgc tttgttcagc atgtcgcgca
     2221 acgtcttcgg cggcatcggc atctccgtct ccaccgcttt ggtcaccgaa cacctgcaga
     2281 tccgccaggc gcatttggtg catgctctgg atcccgccaa tcagccgtac aacgtgctgc
     2341 tacaacaggt gcgccaggcc ctcgtcaacg ccggccagtc ggtcgagcag gccaaccaga
     2401 tggcgccggg ccaggtgttc cagatgctgc aaagccaggt tgccgtgctg gcctacaacg
     2461 atgttttcct gattaccgcc tgcctggcgt tcctcatgat cccaacggcg ttgctgatgt
     2521 cggggatcaa gaccaagtcc ggcggcggag ggcactaggg caatgcgcgg gatcgccacc
     2581 ggcgcgcttg tcgcgtctct cgggctgttt ttgggcgcct gttccgttgg accggacttc
     2641 acgtcgccaa agccgcccga tgcggccaaa tggaacgacc cttcggcttc cggttcggtt
     2701 tccaccgtca gcacccaggc caaccccgat ccgcaatggt ggggcggctt tggcgacgcc
     2761 accctgacgg cgttgattga aaaggctatc gccggcaatc tcgatcttca gcaggccgtg
     2821 cttcgggtcg tggaggcgca gcaaggtgag gtcacggccc gtgcggccgg tctgccctcg
     2881 gtgaacggca atgccagcta catgcgcgag caactgggac tgcgcgggct gctgctgtca
     2941 cgcggcatca atggccagat caacgggctg gcggcgccga actcgacgtt gaaccagttc
     3001 agtccggggc tcggcaattc ggccagcacc gcggccaacg gagtgctgaa ccagctctcg
     3061 caacccgttg acctgtttca gtatggtctc agctcgtcat gggaactgga tctgttcggc
     3121 cgggtccgcc ggtcccgcga gcaggcgaag gccagtacgg aagcccaaat ggaagcaacg
     3181 aacgatgcgc tggtcatgct ggagggtcag gtcgcccagg cctacgtgca g
//
LOCUS       DASWQN010000457         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1759219, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000457 DASWQN010000000
VERSION     DASWQN010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759219"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(338..946)
                     /locus_tag="VGC82_07525"
     CDS             complement(338..946)
                     /locus_tag="VGC82_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423156.1"
                     /translation="MAGDRFRFGIWFLGLCAAAYAQFSVGQSSVAQSSAAEQSTAEGR
                     ATVDRLLNALKAAPDEQAAAGLESQLEQTWLRAGSPAVTLLLSRGLRSLQAGQDDEAQ
                     EAFSDAITLQPDVGEAWHQRALARYHAGDVNGAIRDLQETVRLEPRNFLAFRTLANIA
                     TAREDWEGAYAAWQKVMELDPKTPGGEERLRLLKRRAVGEDT"
     gene            complement(948..>2227)
                     /gene="glcF"
                     /locus_tag="VGC82_07530"
     CDS             complement(948..>2227)
                     /gene="glcF"
                     /locus_tag="VGC82_07530"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY0423157.1"
                     /translation="EQLRDPDTASSNAVLRTCVHCGMCTATCPTFLLLGDELDSPRGR
                     IYLIKDMLETGRPATEEVVRHVDRCLSCLSCMTTCPSGVNYMHLVDHARAYIEQTYRR
                     PLPERVLRSLLGRLLPRPGLFRLALMGARVARPLGRLIPGQSMLAQRLRAMLSMAPAA
                     VPPASSTEKPHVYPAEGPRKARVALLTGCAQQVLMPRINEATIRLLTRIGVEVVVAKG
                     AGCCGALNHHTGQHDQAMRLARANIEAWSREEDLDAIVINASGCGTTVKDYGFMFRSE
                     SETLRARAEKIAGMTVDITEFLSRFGYAPTREAPNLTVAYHSACSLQHGQGVTAEPKA
                     LLSRAGFRVAEPREPHICCGSAGTYNLLQPEIAGRLRDRKLGNLKAIKPDLVAAGNIG
                     CITQLSGDSLPVVHTVELLDWMAGGPRPPGVPH"
BASE COUNT          404 a          720 c          719 g          384 t
ORIGIN      
        1 gttcgcctgg gctcagtgaa aacggtctag ctcattgttt agccagcata ttcacccgtt
       61 cagataatct atctgaacgg gatctgctct agctggtctt gatcgtcgat accagcccgg
      121 gtcgcgctga atctgcactg aaatagcctc cgccgtcgcc cagacttttg ccgctatgac
      181 ggcaattcgg ggcttagggt gggatgccga ccaagacagc gctagtcgga tcaatccggc
      241 tcgcagcgtt atttgtcatc aaacgcttaa acgaaccgat gacaaccgag acacgctcgg
      301 atgacgggaa cgccgaaagc cttgagcgac gccatgatca ggtgtcctcg ccaaccgctc
      361 ggcgcttgag caggcgcaaa cgctcctctc cacccggcgt ctttgggtca agttccatta
      421 ccttctgcca cgcggcatac gcaccctccc agtcttctcg ggcggtggcg atgttggcca
      481 gagtgcggaa cgccaggaag ttcctgggtt ccaggcggac tgtttcctgc aggtcccgga
      541 tcgccccgtt cacgtcgccg gcgtgatagc gggccagggc acgctggtgc cacgcttcgc
      601 ccacatcggg ctgcaaggtt atcgcatccg agaaggcctc ctgggcctcg tcgtcctgac
      661 cggcctgaag cgagcgcaaa ccgcggctga gcagcaacgt caccgccggc gagccggccc
      721 gcagccacgt ctgttccagt tggctttcca gtccggcggc agcttgctca tccggcgcgg
      781 ctttcagcgc attgagcaag cggtccacgg tcgcgcgacc ttcggccgtg gactgttcag
      841 cagccgaaga ctgggccacc gaagactggc ccaccgaaaa ctgggcataa gcggcggcgc
      901 aaaggccgag gaaccagatc ccaaaccgga agcgatcgcc tgccatctca gtgcggcacc
      961 cctggcggcc gaggaccgcc ggccatccag tccaacagtt cgacagtatg tacgaccggc
     1021 aagctgtcgc ccgaaagctg ggtgatgcag ccgatgtttc ccgcagcgac caagtcgggc
     1081 ttgatggctt tcagattgcc cagcttgcgg tcgcgcaggc gcccggcgat ctccggctgc
     1141 aacaggttat aggtgccggc ggatccacag cagatatgcg gctcccgcgg ttcagcaacg
     1201 cgaaaccccg ctcgggacag caacgccttg ggttcggccg tcacgccctg cccgtgctgc
     1261 aggctgcatg cggagtgata ggcaacggtc aggttcggcg cctcacgggt cggcgcgtag
     1321 ccgaagcgcg acaggaactc ggtgatgtcc acggtcatgc cggcgatctt ttcagcgcgg
     1381 gcacgcaagg tctcggactc cgagcggaac atgaagccat agtcctttac ggtcgtgccg
     1441 catccggacg cattgatgac gatcgcatct agatcttcct cccgcgacca cgcctcgata
     1501 ttggcgcgcg ccagacgcat cgcctggtcg tgctggccgg tgtgatggtt caaggcaccg
     1561 cagcatcccg cacctttggc cacgacgacc tccacaccga tgcgtgtcag caggcggatg
     1621 gtggcctcgt tgatgcgtgg catcagcacc tgctgcgcgc agccggtcag cagggcgacg
     1681 cgggccttgc gcgggccctc ggcgggatag acatggggtt tctccgtcga gcttgccggt
     1741 ggcaccgcgg cgggtgccat cgagagcatc gcccgcaggc gttgtgccag catcgactgc
     1801 cccgggatga ggcggcccag cggtcgggcc acacgagcgc ccatcagggc caggcggaac
     1861 aggccgggcc gaggcagcag acggccaagt aacgaccgca gcacacgttc cggcagcggg
     1921 cggcgatacg tctgttcgat ataggcgcgc gcatgatcga ccaggtgcat gtagttcacg
     1981 ccggacgggc aggtcgtcat acaggacagg caggacaggc agcggtccac atggcgcacg
     2041 acctcctcgg tggcgggacg cccggtctcc agcatgtctt tgatcaggta gatgcggcca
     2101 cgcgggctgt cgagctcgtc acccagcagc aggaacgtcg ggcaggtcgc ggtgcacatg
     2161 ccgcaatgca cgcaggtacg caggacggcg ttggacgacg cggtgtccgg gtcgcgcagc
     2221 tgttcgg
//
LOCUS       DASWQN010000458         1890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1099061, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000458 DASWQN010000000
VERSION     DASWQN010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099061"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..906
                     /locus_tag="VGC82_07535"
     CDS             205..906
                     /locus_tag="VGC82_07535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003616559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY0423158.1"
                     /translation="MDNLQPEMLALPSGLARVEWRRSLRARRVSLRIDPTGGGVIVTL
                     PARATRKAGMALLMGHAEWVSSRLAALPEAVIFTEGASVPICGVPYRIRHAPAAKGGA
                     FLLDQELHVTGAVDFLSRRVRDFLRQEARRRLSALAIAKATLIGVVPKRITMKDTTSR
                     WGSCAPDKSLALSWRLVMAPAFVQDYVVAHEVAHLRHMNHGPKFWSLVDELTPHTKAA
                     IPWLRAEGARLLRIG"
     gene            complement(994..1527)
                     /locus_tag="VGC82_07540"
     CDS             complement(994..1527)
                     /locus_tag="VGC82_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0423159.1"
                     /translation="MPNTDGHGIPLPRAAAQSKVPRIVHEIAAVSDRPELAPIVAAWL
                     VDAFGYEGGRTVDQMTALILAPPKGPEETFVLLDQGRPVGTASLAHADLAARPDLTPW
                     LAGVFVEPASRGLGYATALVRCVEAFASAASVPALWLYTWTAEPLYARLGWHRVGLEK
                     DRDQTVVLMTRRLSDRF"
BASE COUNT          358 a          560 c          609 g          363 t
ORIGIN      
        1 tccaccgcca aggctgcgaa cggcaccagg gtcagggccg cggtaaatga acgccggcgc
       61 atcgtgcgga tccgttacgt tatattataa cattagagtt agatgctctg gcgttcaagc
      121 ggtcagccca agtcgtcagc ccaagttgtc agcccaagcg gtgagccatt gacgaatcga
      181 ttatatctct gtgcattcag ggtcatggat aatctgcagc ctgagatgct tgccctgcct
      241 tccggactgg ctcgtgtgga atggcgccgc agcctccggg cgcgccgggt gtctctgcga
      301 atagacccca ccggcggcgg cgttatcgtg accttgccgg cccgggcgac acgcaaagcc
      361 ggcatggcat tgttgatggg ccacgccgag tgggtgtcca gccgactggc ggctctgccg
      421 gaggcggtga tcttcaccga aggtgcgtcg gtgccgatct gcggggtgcc ctatcgcatc
      481 cgccatgcgc cggcggccaa aggtggcgca ttcctgctgg accaggaact gcatgtgacg
      541 ggtgcggtgg attttttgtc ccgccgggtt cgcgacttcc ttcgccagga agcgaggcgg
      601 cggcttagcg cactcgcgat agccaaagcc acgctgatcg gcgttgtgcc gaagcgcatc
      661 acgatgaagg acaccaccag ccggtgggga agctgtgcgc ctgacaagtc gctggcgttg
      721 agctggcgcc tcgtgatggc accggcgttc gtgcaggatt acgtcgtggc acacgaggtc
      781 gcgcaccttc ggcacatgaa tcacggcccc aaattctggt cgctggtgga tgaactgacg
      841 ccccatacca aagccgcgat tccctggctg cgagccgaag gcgcgaggtt gttgcgcatc
      901 ggatagggtg cttcggccaa ggcaaccgaa tcgatctggt gcccagctgg gtcgcgcaaa
      961 gacttcggaa acgccggggc ggaaaccagg cgctcagaac ctgtcggaca gacgccgcgt
     1021 catcagcacg accgtctggt ctctgtcctt ttccaagcct acgcggtgcc agccaaggcg
     1081 tgcatacagc ggttccgccg tccaggtgta aagccatagc gccggcaccg aggcggccga
     1141 cgcaaacgct tcgacgcacc gtaccaacgc ggtcgcatac ccaaggccac gcgaggctgg
     1201 ctcgacaaag actccagcga gccacggtgt cagatccggc cgagcggcca aatcggcgtg
     1261 ggcaaggctg gcggtcccga caggcctgcc ctggtcgagt agaacaaacg tttcttcagg
     1321 gcctttgggc ggtgccagga tgagcgccgt catctgatcg actgtccggc caccctcgta
     1381 tccaaaggca tccaccaacc atgccgcgac aatgggtgcg agttcgggtc ggtcggaaac
     1441 ggcggcgatt tcgtggacta tccttggaac cttgctctga gccgcggccc tcggcaatgg
     1501 gatgccatgg ccatcggtat tgggcattcg cggatcgaag gtccgccgag gcgtcggcct
     1561 ggtcccatcc ggtgcaggcg cggcaggccg cagccggcgg cggacaacgg ttggccgccg
     1621 aacccgtcgc tgctctcggt cacgtaattg aggtggcttg tgtaaaagtg tctgtcatgg
     1681 gtaagcacta tgcttctccc ccggtctgcc ggtcttttat tgcggacacg cgggaatggc
     1741 agaaagacta ttcggttgtt gtctgggtac caataaaact aaacatttac agaacagtat
     1801 tcggaaatgg caccagatcg gctgcggtat agttgcccga ccacatgatg ggtgagggtt
     1861 ttcactaggg cctgttagag cttgatgact
//
LOCUS       DASWQN010000459         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1759811, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000459 DASWQN010000000
VERSION     DASWQN010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759811"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VGC82_07545"
     CDS             <1..875
                     /locus_tag="VGC82_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0423160.1"
                     /translation="RQMRKQLADGGKIQSGPAADPGPLSMLVVGVAGVAFLIAEGAVL
                     SAQGVPAVAVILVAVFAASTVSSIAGFAFSALCGALLLHVIDSPVYAVNVMIICSIAI
                     QLLGVATLWRSIDWRSLGLFLVGGFPGVPAGVYLLLNLPTTTYRHVIGGLLIAYGGYL
                     LLRWPARSRRTGPLTDACAGFLGGITGGLAGFPGAFVTIWCGLKGWDKARQRGVYQPF
                     ILIMQPVTLVAIRLMQPSSPVQGQLDWKAFVFVPAALLGAWFGLRIFKRLTDRQFGLV
                     VNALLILSGIGLVL"
     gene            929..1963
                     /gene="dctP"
                     /locus_tag="VGC82_07550"
     CDS             929..1963
                     /gene="dctP"
                     /locus_tag="VGC82_07550"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY0423161.1"
                     /translation="MVWSTSRRTFLASAMAMVAAPALRTGAAAADAPLLLRCSLETAP
                     SHTRNAIIRDYLGRIEAAAGGRIKTQLFESGQLFPDLQVGKALLQGQVEMAAPGSWSL
                     TGIVPDADFFQLPILYGRSIETVHRACDGKPGQLLAQQIERQLRSRVIGPWLDLGFFN
                     WYSTNRPLNSYGDLKGLKIRNSGGAGQAWRTQFVGAIPNTTPLPNVQLGLSQGTFDGL
                     ITTYETIASGQFWESGIRHALEDHQFTGEYIPMVSLAFWQRLPSDLQQAFTDLWQEKV
                     AVYRADMAAAQSRARELVRAHGVSIVVPSADELAATRQQMMASQDQVANLSKISSEMV
                     SAIAAGLAGG"
     gene            1960..2508
                     /locus_tag="VGC82_07555"
     CDS             1960..2508
                     /locus_tag="VGC82_07555"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY0423162.1"
                     /translation="MKTLKAADTMKAWDRAERTLVGVLGLAALAFALWQVASRYFFPR
                     ESISYAEEVIVYLMIWAIMIVSSQLVRTDSHVRPDLVLHVVPVGVARWMEVFNCFAAM
                     AFCGALIWYGWQIVEIAWLIDERSASDLRFPMWIYYSALPVGGLLMLIRYAIRIAGLV
                     APSGRNAMPAHRPGGHELPGID"
     gene            2518..>2796
                     /locus_tag="VGC82_07560"
     CDS             2518..>2796
                     /locus_tag="VGC82_07560"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY0423163.1"
                     /translation="MTSFLMLFVGLLATGMPVFLVLGLCAGVMFVTSGQPPVGAAQLV
                     INELNSPTLMALPLFVMAATFMRFGGIANALVNLAAAWLGGLPGSLGLV"
BASE COUNT          446 a          841 c          904 g          605 t
ORIGIN      
        1 cgcgacagat gcggaagcag ctggccgacg gcggcaaaat ccagagcggc cctgcggccg
       61 atccgggccc cctaagcatg ctcgtcgtcg gggttgctgg cgttgcattc ttgatcgcgg
      121 aaggcgcggt gctctcggcg caaggtgtcc cggctgtcgc ggttatcctg gttgccgttt
      181 ttgcggcttc caccgtctcc agcatcgccg ggtttgcttt ctcggcgctg tgcggtgcct
      241 tgctgcttca cgtcatcgac agcccggtct atgcggtgaa tgtgatgata atctgcagca
      301 ttgcgattca attgctcggt gtcgccacgc tctggcgttc gattgattgg cgcagtctgg
      361 gacttttcct ggttggcggt tttcccggag tgcccgccgg cgtctatctg ttgctgaatc
      421 ttccgaccac cacctatcgg cacgtgatcg gaggcctgct cattgcctat ggcggctatc
      481 tcttgctgcg ttggcctgcc cgatcgcgcc ggaccggacc gctgacggac gcctgcgccg
      541 gctttctcgg cggcatcacc ggtgggctcg ccggctttcc cggtgcgttc gtgacgatct
      601 ggtgcggtct gaagggatgg gacaaggcgc gtcagcgcgg tgtgtatcag ccgttcatcc
      661 tcatcatgca gccggtgaca ctggtcgcga tccgcctgat gcagccgtcg tccccggttc
      721 aggggcaact ggactggaag gcgtttgtgt tcgttccggc tgcgctgctc ggcgcctggt
      781 ttggactgcg catcttcaaa cggctcacgg atcgacagtt cggactggtc gtcaacgcgc
      841 tgctgatctt gtccggcatc ggactcgtcc tttgagcgac ctgaactcgc atcatcatcc
      901 gcacgtcaac aagacaggag caacaaacat ggtttggtcg acctcccgcc gcacattctt
      961 ggcttcggca atggctatgg tggcagcgcc cgccctccgc acgggggcag ccgcggccga
     1021 cgcaccgttg ctgctgcgct gttcgctgga aaccgcgcca tcgcatacgc gcaacgcaat
     1081 catcagggac tatctcggaa gaatcgaggc ggccgcaggt ggaaggatca aaacccaatt
     1141 attcgaaagc gggcaactct ttcccgacct gcaggttggc aaggcgctgc tgcaggggca
     1201 ggtcgagatg gcggcgccgg gaagctggtc gctgaccggc atcgtgccgg acgccgattt
     1261 ctttcagctt ccgatccttt acggccgttc gatcgaaacg gtgcatcgcg cctgcgatgg
     1321 caaacccggg cagctgctcg cgcaacagat cgaacggcaa ctgcgttcgc gcgtgatcgg
     1381 tccgtggctc gatctgggat ttttcaactg gtacagcacg aacaggccgc tgaattccta
     1441 cggtgacctc aagggcctga aaattcgcaa cagcggcgga gcgggacagg cgtggcggac
     1501 acagttcgtg ggcgcgatcc cgaataccac gccgttgccg aacgtgcagc tcggactttc
     1561 gcaaggcacg ttcgacgggc tgatcacgac ctatgagacc atcgccagcg gacaattctg
     1621 ggaatcggga atccgccatg ccctggaaga tcatcagttc accggtgaat acattccgat
     1681 ggtgagtctc gccttctggc aaagactgcc ttccgatctc cagcaggcat ttaccgatct
     1741 ctggcaggag aaggttgcgg tctaccgcgc cgacatggcg gccgcacaat cgcgcgcacg
     1801 cgaattggtg cgggcgcacg gcgtcagcat tgtcgtgccg tcggcggacg agctggcggc
     1861 gacgcggcag cagatgatgg ccagtcagga ccaggtggcg aatctgtcga agatatcgtc
     1921 cgaaatggtc tccgccattg cagcgggact cgctggcgga tgaagacctt gaaggctgct
     1981 gacacgatga aggcctggga tcgggcggag cggacgttgg tcggggtgct cggcctggcc
     2041 gcgctcgcct tcgccctttg gcaggtcgcg tcgcgctatt tctttccgcg ggaatcgatc
     2101 agctatgccg aggaagtcat cgtctacctg atgatctggg cgatcatgat cgtgtccagt
     2161 cagctggtcc gcaccgacag ccatgtcagg cccgacctcg ttctccacgt cgtcccggtc
     2221 ggcgtggcgc gatggatgga agtattcaat tgcttcgcgg cgatggcttt ctgcggcgcg
     2281 ctgatctggt acggctggca gatcgtcgaa atcgcctggc tgatcgacga gcgcagcgcc
     2341 tcggatctgc ggtttccgat gtggatctat tattcggcgc tgccggtcgg cggcctgttg
     2401 atgttgatcc gctatgccat ccgcatcgcc gggctggttg cgccgtcggg ccggaatgcg
     2461 atgcccgcgc atcgtccggg cggccatgaa ctgccaggca tcgattgagc cggcgacatg
     2521 accagtttcc tcatgctgtt tgtcggactg ctggctaccg gaatgccggt ctttctggtg
     2581 ctggggcttt gcgccggcgt catgttcgtg acttccggtc agccacctgt gggcgccgcg
     2641 caactcgtca tcaatgagct caattcgccg acgttgatgg cgctgccgct attcgtgatg
     2701 gcggcgactt tcatgcggtt cggggggatc gccaacgcgc tggtgaatct ggcggcggcc
     2761 tggctcggtg ggctcccggg atcccttgga ctcgtt
//
LOCUS       DASWQN010000460         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2551749, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000460 DASWQN010000000
VERSION     DASWQN010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2551749"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VGC82_07565"
     CDS             complement(<1..1022)
                     /locus_tag="VGC82_07565"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0423164.1"
                     /translation="MAKAFAGIRVLDFSQVLAGPSCTQQLALLGADVIKIEEPIAGDQ
                     SRGIMADNDIGRIGMSPYFLSMNANKRSMTLDMKHPRAAEVLARLTAGAAIVVQNFRP
                     GVLTRLDFGYQAVCAIKPDIIYCSISGYGQEGPFATAPAYDGAVQAVSGLMAVTGQPD
                     GPPTRVGSSIVDLSTAMMAAFAIASALFRRSVTGEGQHLDVAMFDTALALMAPMANVW
                     MNTGRAPERLGNASPAYVPTADSFPIGGGEILIAALTERQWQGICTAINRPDLPTDPR
                     FTTTNARRANAPALRAVLIEAFASADAAEWEKRLSAAGVPAAPILTLQDALKHPQLAH
                     RNIVSRL"
BASE COUNT          305 a          496 c          523 g          296 t
ORIGIN      
        1 agccgcgaaa cgatgttgcg gtgagcgagt tgcgggtgct tcagcgcatc ctgcagggtg
       61 aggatcggtg ctgccggcac gcccgcggcg gataggcgct tctcccattc ggccgcgtcg
      121 gcggatgcga acgcctcgat cagcaccgcc cgtagcgccg gtgcgttggc gcggcgcgcg
      181 ttcgtcgtgg tgaagcgtgg atcggtcggc aggtccggac gattgatggc ggtgcagatg
      241 ccctgccact gccgctccgt cagtgccgcg atcaggatct cgccgccacc gatcggaaac
      301 gaatccgctg tgggtacata ggcgggtgat gcgttgccca aacgctccgg tgcacggccg
      361 gtattcatcc agacattcgc catcggcgcc atcagcgcca acgcggtatc gaacattgcg
      421 acatccagat gttgtccctc gcccgtcacc gaacggcgga acagtgcgga ggcgatcgca
      481 aacgccgcca tcattgcggt cgatagatca acgatggagc tgccgacccg cgtcggcggc
      541 ccatccggct ggccggtcac cgccatcagg ccggaaaccg cctgcaccgc gccgtcatat
      601 gcgggcgccg tcgcaaaagg cccctcctgt ccgtagccgg aaatcgagca gtagatgatg
      661 tccggcttga ttgcgcacac cgcctgatag ccaaagtcga gtctggtcag cacgccgggg
      721 cggaagttct gcacgacgat ggcggcgccg gcggtgaggc gggcaagcac ctcggcggcg
      781 cgcgggtgct tcatgtcgag cgtcatcgac cgcttgttag cgttcatcga caggaagtac
      841 ggcgacatgc cgatccggcc aatgtcattg tcagccatga tgccgcggct ctggtcacca
      901 gcgataggtt cctcgatctt gatgacgtca gcgccgagga gggcgagttg ctgggtgcag
      961 gatggcccag cgaggacctg cgagaagtcg agcacgcgga tgcccgcgaa ggccttggcc
     1021 attattgtta tctcccccca atcgaactcg ggcatcgccg ggggcgatcg tatctgattt
     1081 ccgcctgccc ggggactgcc aggttgggat cgctgcgaat ggttcgctgc gccgtccgca
     1141 aggcgatcga ttttcaaact ttccctacct acccaaaaga caagccattg attcagcttg
     1201 ggaaaaagct tgtagagtga atcggccaac cccaagcttc ggagagagac cgcgcttcgg
     1261 agagagattt gccgtatcat tccggctctg aaaccccaag cctgacccgc aaaaccgcct
     1321 ggaacctggc ctttttagag gcccgaaatc cggcggagaa tgtttgtggg ggaggtgact
     1381 ggagcgggcg aaaggattcg aaccctcggc cccaaccttg ccaagagtag catattgcgg
     1441 gtcatacgca ccgacggcca accggtgacc tggcggagca cacataggtt tccgccgtcg
     1501 tgaggaacca ggcagcgaca tggtgacgcc ctgccatgca ttcttaatcc cgtgatcctg
     1561 actttcttgc acgcggtggc ggccgaggcc gggaggctac cgccggtctg attggtatcg
//
LOCUS       DASWQN010000461         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2023740, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000461 DASWQN010000000
VERSION     DASWQN010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2023740"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VGC82_07570"
     CDS             <1..795
                     /locus_tag="VGC82_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0423165.1"
                     /translation="AWDAFNPAAVDRTLVPLVKAAAMVERNGRDYALYLGGVFHDDPA
                     FRDAANYWAEEEVQHGDALAKWAVLADPTWNFAAAFDRYRAGYTIKTDADASIRGSRT
                     GELIARCMVETGTSSYYTALAEATSEPVLKQVCKLIAADEYRHFKLFYDHMRRYLSRE
                     NLGVMKRLRIALGRIGESEDDELAYAYYCGNDAASQPYRHNRCTAAYMAGAMALYRYR
                     HIERGMGMVLKTVGLPPRGRISAGATKVAWHLLQWRQRRFVQQLAG"
     gene            complement(813..1598)
                     /locus_tag="VGC82_07575"
     CDS             complement(813..1598)
                     /locus_tag="VGC82_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY0423166.1"
                     /translation="MTVRNVAREKLEQGQLSLGVGIRTTRSVEVAKMMATAGFDWLFL
                     DMEHGTMSLDACAQISAAALDAGIAPIARVPNGQYSIATRALDNGAMGIVMPHVDTAA
                     EAREVVERLKYPPIGHRSVGGWGPHYQLAKLSTGEAVTALNAANLTVVMLETPTAIAN
                     AAEIAAVPGVDVLLIGSNDLCAEMGIPGDFGNERLAEAYRTMIAACTKAGKFPGMAGI
                     YNESIMPRYIDMGARFILSGQDAAFMLAGAQSRTGFLRKTLSR"
     gene            complement(1800..>2997)
                     /locus_tag="VGC82_07580"
     CDS             complement(1800..>2997)
                     /locus_tag="VGC82_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0423167.1"
                     /translation="TIQSEAAAAHAAGDFNIETDAADSRVTVRWGDRVGHAVARPGYG
                     SILLRPGIAPGFDPQPISRHFPHAGQPWPIGDAVETGAAPPATFADASDAFFAGSPGA
                     YGVLVASPDRVLAERYSDFGRADRATPSWSMTKAITCTVIGRMIHDGWLRSVYDPAPA
                     PLWTDERAIHRLITLDHLLRMRSGLGCPVRHEDGRTTIGFENIAVYQDAGDAFEAAQR
                     SLVATIPGSVFRYVNSGMNVLGAVIRDQIERRGLPYFETAYGLLVDRIGMSGYQYSAD
                     LAGNLIASGAGYATLRDYAKLGVLYLNDGIWNQERLLPAGWVDYALTPTHTGTSYAAC
                     FRTNFDRAFPSLPSDTAWAAGASDQKIFILRRHRLTVAVSNETDHAMDMAALDRLIAM
                     AIDCYS"
BASE COUNT          552 a          981 c          967 g          497 t
ORIGIN      
        1 gcctgggacg cgtttaatcc cgctgccgtg gaccgcacac tcgtgcccct cgtcaaagcc
       61 gcggcgatgg tcgagcgcaa cggccgcgac tacgccctgt acctcggcgg cgtcttccac
      121 gacgaccccg ccttccgcga tgccgccaac tattgggccg aggaagaggt ccagcacggc
      181 gacgccctcg ccaaatgggc cgtactggcc gatccgacat ggaatttcgc cgccgccttc
      241 gaccgctacc gcgccggcta caccatcaag accgacgccg acgcgtcgat ccggggctcg
      301 cggaccggcg agcttattgc ccgctgcatg gtcgaaaccg gcacctccag ctactacacc
      361 gcgctggccg aggcgacgtc ggaacccgtg ctgaaacagg tgtgcaagct gatcgcggcc
      421 gacgagtacc ggcatttcaa gctgttctac gaccatatgc ggcggtacct gtcgcgcgag
      481 aacctcggcg tgatgaagcg ccttcgcatc gcgttgggcc gcatcggcga gtcggaggac
      541 gacgaactgg cctacgctta ttattgcggc aacgacgcgg catcgcagcc gtatcgccac
      601 aaccgctgca ccgcggccta tatggccggg gccatggcgc tttaccgcta ccgccacatc
      661 gaacgcggca tgggaatggt gctgaagacg gtcggcctgc cgccgcgcgg ccggatttca
      721 gcaggggcta caaaggtcgc ctggcacctg ctgcagtggc gccagcgccg tttcgtgcag
      781 caactggccg gatagatcac agccgaccgg acctaacgcg aaagggtctt gcgcagaaat
      841 ccggtcctgg actgggcgcc ggcgagcatg aatgccgcgt cctgcccgga gaggataaac
      901 cgagcgccca tgtcgatata gcgcggcatg atcgactcgt tgtagatgcc ggccatgccg
      961 gggaatttcc ccgccttcgt gcaggcggcg atcatcgtgc ggtaggcctc ggccaggcgt
     1021 tcgttgccga agtcgccggg aatgcccatc tcggcgcaaa gatcgttgga cccgatcagc
     1081 agcacgtcca cgccgggcac cgcagcgatc tcggccgcgt tggcgatcgc cgtcggggtt
     1141 tccagcatca ccacggtcag gttggcggca ttcagcgcgg tgaccgcctc gccggtgctg
     1201 agcttcgcga gttgatagtg cggcccccag ccgccgacgg aacggtggcc gatcggcgga
     1261 tatttcagcc tctcgaccac ttcacgcgct tcggcggcgg tatcgacatg cggcatgacg
     1321 atgcccatcg cgccgttgtc cagcgcccgc gtggcaatcg aatactgccc gttcggcacg
     1381 cgggcgatcg gtgcgatgcc ggcatccagg gccgcggccg agatctgggc gcaggcatcg
     1441 agcgacatcg tgccgtgctc catatccagg aacagccagt cgaaaccggc ggtggccatc
     1501 atcttggcca cctccacgct gcgggtcgtg cgaatgccga cacccagcga caattgcccc
     1561 tgctccagct tctcacgcgc gacgttgcgg acggtcatgt gcgtttcctt ccttgagcat
     1621 gatcacgcgc ggcggaacgt cgaagcgggt gaatcatgcg atcaacaaag ccctaaagca
     1681 tgatcacgcg cggcgggacg tcgaagcggg tcaatcatgc gatcaacaaa gccctatggt
     1741 atgatcacgc gcggcggaac gtcgaagcgg gtcgatcatg cgatcaaaca aagagtcgtc
     1801 taagaatagc agtcgatcgc cattgcgatc agtcgatcga gtgcggccat atccatcgca
     1861 tggtcggtct cgttggatac cgccaccgtc agccggtgac gccgcaggat gaagattttc
     1921 tgatccgacg caccggcggc ccaggcagta tcggacggca ggctgggaaa cgctcggtcg
     1981 aagttggtcc ggaagcaggc ggcatagctg gtgccggtgt gggtgggggt cagcgcatag
     2041 tccacccaac ccgcgggcag cagccgctcc tggttccaga tgccatcgtt cagatacagc
     2101 acgcccagtt tcgcatagtc ccgcaacgtc gcgtagccgg cgcccgaggc aatcagattg
     2161 cctgccaggt cggccgaata ctggtagccg ctcatgccga tccgatccac cagcaggccg
     2221 taggcggtct cgaaatacgg caggccacga cgctcgatct ggtcgcggat gacggcaccc
     2281 agcacgttca tgccgctgtt cacataacgg aacaccgaac cggggatggt tgcaacgagc
     2341 gacctttgag cggcctcgaa tgcgtcgccg gcatcctgat aaactgcgat attctcgaac
     2401 ccgatggtgg ttcggccgtc ttcatggcgc acgggacaac cgaggcccga ccgcatccgc
     2461 agcaggtggt ctaacgtgat caacctgtgg atggcccgct cgtcggtcca aagcggcgcc
     2521 ggggcgggat cgtaaaccga gcgcagccag ccgtcatgga tcattcggcc aatgaccgtg
     2581 caggtaatcg ccttggtcat cgaccagctt ggcgttgctc ggtcggcccg cccgaagtca
     2641 ctgtatcgtt ccgccaggac ccggtccggg gaggcaacca gcacgccgta ggcgccgggc
     2701 gaaccggcga agaatgcgtc cgacgcgtcg gcaaacgtag cgggtggggc cgcgcctgtt
     2761 tccaccgcat cgccgatcgg ccagggctga ccggcatgcg ggaagtgacg actgatgggc
     2821 tgcggatcga acccgggcgc tatgccggga cgcagcagga tgctgccgta tccgggacgc
     2881 gccacggcat ggccgacgcg atccccccaa cgcacggtca cgcggctgtc cgccgcgtcc
     2941 gtctcgatgt tgaagtcacc ggcggcgtgg gcggcggcgg cttccgactg gatagtc
//
LOCUS       DASWQN010000462         2194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2287738, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000462 DASWQN010000000
VERSION     DASWQN010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2287738"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VGC82_07585"
     CDS             complement(<1..782)
                     /locus_tag="VGC82_07585"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY0423168.1"
                     /translation="MIASNAWLLAGMTALFLSLGYLGVPVAFALIAGVLVGTAFTPIS
                     LQSMMGQLFNGIDSEALMAIPFFLLVGELMTSANVVIRIAELSQALVGHIRGGLAQVT
                     TVFSMFFSGMSGSSSADVAVLSRTMAKPMAREGYRPEFTAALIAAASTIAALVPPSIM
                     AVVYGAIGNVSIAGLFLGGIAPGLFIGFGLMIYCYLFGPRGLQRPRATFGQLITAGRS
                     AALPMMIPVILLGGILTGWFTPTEAGVIAIAYILLIVIPLLNP"
     gene            complement(779..1324)
                     /locus_tag="VGC82_07590"
     CDS             complement(779..1324)
                     /locus_tag="VGC82_07590"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY0423169.1"
                     /translation="MTAPIVLETDRHLKWSAFDRLEKWLMILCGLCLTAFTVSTFIDV
                     VTREIGHPLLWLQEVTSTFFTYGVFIGTAVATRRNDHLFLSALTEKMSGATRRFFEVF
                     NRLVVLLVGVGMVWFGWQNVLTGLGSFRMPSMLPMSYLYLGIPLCGVLVALFSLEQIV
                     NGWRNGFSAPVGEGVVRDTGL"
     gene            complement(1331..>2194)
                     /locus_tag="VGC82_07595"
     CDS             complement(1331..>2194)
                     /locus_tag="VGC82_07595"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY0423170.1"
                     /translation="ADLFATKLSELSKGSMEIDQFPGAQLGQEPQMLQKIRTGDIDFI
                     FSATANAATLSPQSGVLSIHYLFRSEDHLVHAIADPKLVNAMRDMFGETVKDAHVLTL
                     LTLGLRNLYGKKEIHSIADVKNLKIRVQATPTEDAMFPAYGAQTVHMPFGSVYTSLQT
                     GVVDMAENGINVYDTNKHYEVAPVMSLTGHEANANLLWVSGKVWDSLNAEQRGWVEGA
                     AKEVATKEPALAIALESKSRAKLEKLGVKFVTDVDKAGFIAAAKPLQDKIASDLGPHA
                     TKILEIARSIQ"
BASE COUNT          449 a          722 c          653 g          370 t
ORIGIN      
        1 ggattcagca gcgggatgac gatcagcagg atatacgcga tggcgatgac gcccgcctcg
       61 gtcggcgtga accagccggt caggatgccg cccagcagga ttaccgggat catcatcggc
      121 agcgccgccg accgcccggc cgtaattagc tgcccgaacg tcgcgcgcgg ccgctgcagc
      181 ccgcgcggtc cgaacaggta acagtagatc atcagcccga agccgatgaa caaccctggc
      241 gcgatgcctc ctaggaacag cccggcgatc gaaacgttgc cgatcgcgcc atagaccacc
      301 gccatgatcg acggcggcac cagcgccgcg atggtggagg cggcggcgat cagcgcggcg
      361 gtgaactccg gccggtaacc ctcgcgcgcc atcggtttcg ccatagtacg cgacagcacg
      421 gcgacatcgg cggacgacga cccagacatg ccggagaaga acatcgagaa caccgtcgtc
      481 acctgcgcca ggccgccgcg gatatggccg accagcgcct gcgacagctc cgcgattcgg
      541 atgacgacat tggcggaagt catcaactcg ccgaccagca ggaagaacgg gatcgccatc
      601 aacgcttccg aatcgatgcc gttgaacaac tgccccatca tcgactgcag gctgatcggc
      661 gtgaacgccg ttcccaccag cacgccggcg atcagcgcga acgccaccgg cacgcccagg
      721 tatcccagcg acaggaacag cgccgtcatg cccgcgagaa gccacgcgtt cgacgcgatc
      781 acaggccggt atcccgcacc acgccctcgc cgaccggcgc ggagaatccg ttgcgccacc
      841 cgttgacgat ctgctccagg gagaacaacg ccaccagcac cccgcacagc gggatgccaa
      901 gataaaggta agacatcggc agcatcgagg gcatccggaa actgccgagc ccggtcaata
      961 cattctgcca gccaaaccac accatcccaa cacccacgag cagaacgacc aagcggttga
     1021 agacttcgaa gaatctccgc gtcgcgccgg acatcttctc ggtcagcgcc gacaggaaca
     1081 gatgatcgtt ccgtcgcgtg gcgaccgcgg tgccgataaa tacgccgtag gtgaaaaagg
     1141 tcgaggtaac ctcctgcagc cacaacaggg gatgtccgat ctcgcgggtg accacgtcga
     1201 tgaaagtcga gacggtgaac gccgtcaggc acagcccgca caggatcatc agccactttt
     1261 caagcctgtc gaaggcggac cacttgaggt ggcggtccgt ctccaggacg atcggtgcgg
     1321 tcatgacggc ctattgaatc gaccgggcga tctccaggat cttggtcgca tgcgggccga
     1381 ggtccgaggc gatcttgtcc tgcaacggct tggccgcggc gatgaagccg gccttgtcca
     1441 cgtcggtcac gaacttgacc ccgagttttt ccagcttggc gcgcgacttg gattccagtg
     1501 cgatcgccag ggcgggttcc ttggtcgcga cttccttcgc cgcaccctcg acccagcccc
     1561 gctgttcggc attgagggaa tcccatacct tgccgctgac ccacagcagg ttggcgttcg
     1621 cctcatgccc ggtcagcgac atgactggtg cgacctcgta atgcttgttc gtgtcgtaaa
     1681 cgttgatgcc attttcggcc atgtccacga ctcccgtctg caaactggta tagacgctgc
     1741 cgaatggcat gtgcaccgtc tgcgcgccat aagccgggaa catcgcgtcc tcggtcggcg
     1801 tcgcctgcac ccggatcttc aagttcttca cgtcggcgat cgagtggatt tccttcttgc
     1861 cgtaaaggtt ccgcaaaccc agcgtcagca gcgtcaggac atgcgcgtcc ttgacggtct
     1921 cgccgaacat gtcccgcatc gcgttcacca gctttggatc ggcgatcgcg tggaccagat
     1981 ggtcctctga ccggaacagg tagtggatcg aaagcacccc ggattgcggc gacaaggtcg
     2041 ctgcgttggc cgtcgcactg aagatgaagt cgatgtctcc ggtgcggatt ttctgcagca
     2101 tctgcggttc ctggcccaac tgggcgccgg gaaactggtc aatctccatc gagcctttgc
     2161 tgagctccga cagtttcgtc gcgaacaggt cggc
//
LOCUS       DASWQN010000463         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2287794, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000463 DASWQN010000000
VERSION     DASWQN010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2287794"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..>1613)
                     /locus_tag="VGC82_07600"
     CDS             complement(377..>1613)
                     /locus_tag="VGC82_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HEY0423171.1"
                     /translation="GWTGLTDCFAHLRTGEPAEDRQLVMTGILADGLNLGLTRMAEAC
                     RGVSLGRLAWMADWHIRDETYALALQRLVEHQHRQPLAAQFGGGFTSSSDGQFFQAGG
                     FGRDAGTVNSHYGREPGVKFYTHLSDRFAPFHTRAISATAGEAIHVLDGLLHHAGAAV
                     DGADPRWTHHTDGGGVSDSVFALCALLGFRFAPRIPDLKDRKLHSFQPPASYPALAPL
                     IGGRIDVALIRAHWTEILRLALSIRTGTVAASVILRQLGAYPRQNGLALALRELGRLE
                     RTLFTLDWLEDPAFRRQAGHELNKGEARNSLARAVFIHRLGEIRDRTYENQQHRASGL
                     NLVVAAIILWNTRYLERAVAALQAATPVTDDLLAHLSPLGWEHINLTGDYVWASDMSE
                     TIDGLRALRTAPSDLRQVA"
BASE COUNT          299 a          478 c          580 g          256 t
ORIGIN      
        1 gacggtacgc gaggccgccg gacggctgaa agtcggcaaa accgcactct acaaggcgtt
       61 cggccgcgct aaggcagatc cacaagcgta agttcaaact tcgttcgctt ctgccgtacg
      121 cagcgatcac tttcttgcag tgaccccatc atagcggcgt tggtttggcg ggctgatgcc
      181 ggtacgccag cagcgactca tgatccggcc gtctgtcgat atcacgtgag ccacactccc
      241 tgcagaccag cggaagcctg tccagacggc gaccggcgcc gaagaatttg atcatgtcgc
      301 acggatcgaa caggccggta tggccggaac taacggttcg tgcgagattg tagcataagc
      361 ggacaaaacc agaacattag gcaacttggc gaaggtcgga gggggcggta cgcagggctc
      421 tcaatccgtc gatggtttcc gacatatcgg atgcccagac gtaatcgccg gtgaggttga
      481 tgtgttccca tcccaggggc gacagatggg cgagaaggtc atcggtcacc ggggtggcgg
      541 cctgcaaggc ggcaacggca cgctcaaggt agcgggtgtt ccacaggatg atggcggcaa
      601 ccaccagatt cagcccggac gcccggtgct gttggttttc gtaggtgcgg tcgcggattt
      661 cacccagacg atggatgaag acggcgcggg cgaggctgtt tcgggcttcg cccttgttca
      721 actcatggcc ggcctgccgg cggaaagccg gatcctccag ccagtcgagg gtgaacaggg
      781 tccgttccag acgccccagt tcccgcaagg ccaaggcgag accgttctgt cggggatagg
      841 cgccgagttg ccgaaggatg accgaggcgg cgaccgtgcc ggtccggatc gacagggcca
      901 gccgcaggat ctcggtccag tgggcgcgga tcagggcgac gtcgatgcgc ccgccgatca
      961 ggggagccag ggcgggatag gaggccgggg gctggaaact gtggagcttg cggtccttga
     1021 gatcgggaat gcgcggcgcg aagcggaagc cgagcagggc acacagcgcg aagacgctgt
     1081 ccgagacccc acccccgtcg gtgtggtggg tccagcgtgg atccgcgccg tccacggcgg
     1141 ctccggcgtg atgcagcagg ccgtccagga catggatggc ttcgccggcg gtggcggaga
     1201 tcgcccgggt gtggaacggc gcgaagcgat cggaaagatg ggtgtagaac ttcacgcccg
     1261 gctcccggcc atagtggctg ttgacggtgc cggcgtcgcg gccgaagccg ccggcctgaa
     1321 agaactggcc atccgaggag gacgtgaagc cgccgccgaa ctgggccgcc aggggctggc
     1381 gatgctggtg ttcgaccagt cgctggagcg ccagggcata ggtttcatcc cggatgtgcc
     1441 agtccgccat ccaggccagc cggccgaggc tgacgccacg gcaggcttcc gccatgcggg
     1501 tgaggccgag gttgaggccg tcggccagga tcccggtcat caccagctgg cggtcctcgg
     1561 ccggttcccc ggtgcgcaga tgggcgaagc aatcggtgag gccggtccac ccg
//
LOCUS       DASWQN010000464         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_176687, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000464 DASWQN010000000
VERSION     DASWQN010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176687"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1904
                     /locus_tag="VGC82_07605"
     CDS             <1..1904
                     /locus_tag="VGC82_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY0423172.1"
                     /translation="RHAKRLADQLRAPWTALSVETARPGASQKDGVPKDNVAEALRLA
                     QLLGAEPVLIPGQDVADTVIDYARANNVTHLIVGKSDRPTWLQFLLGSITQRLINRAG
                     GINIHVIDVPAEPSARPGSAAPVAPAAVPGAGFDPRAFVIASLLVGVAIPVAIGLHRF
                     LGITNIALVVLTAILLAAVLHGLGPSLLACFLAVAAYNFFFLPPFYTFTIADPENVVA
                     LFFFAVVAVVVSNLAARVRGQALSARLRARQTDDLYQFSRKLAGAVALDDVLWATAHQ
                     IAMMLKVRVVLLLPDKDSIAVRAGFPPDDMLDEADLAAAKWSWEKNHIAGRGSDTLPG
                     GKWLFQPLRTGRGPLGVVGIIRDEPGPLLTPDQERLLDALADQAALAIERVNLARDLH
                     LSRLQVETDRLRSALLTSISHDLRTPLASILGSATSLRSQSLDNATRDALLATIIEES
                     DRLNRFIGNLLDMTRLESGALKPRTGLNELSDVIGAVLQRAAKILAGHRIAVDLPRGL
                     PMLALDVVLFEQVLFNLLDNAAKYAPAGSLITIAAGHDRDTVTLRILDEGDGIPPDDV
                     ERIFEKFYRSAGGDRRRAGTGLGWRSAVALSKRWMERSPPVTGPTAKGPCFRSSCQSR
                     STRCRRMRK"
     gene            1909..2616
                     /locus_tag="VGC82_07610"
     CDS             1909..2616
                     /locus_tag="VGC82_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0423173.1"
                     /translation="MSGGSAPLRVLVVDDEPAIRRFLRAGLSSQGYVVSELEMGLPAV
                     DVARRKGADLIVLDLGLPDIDGIEVIGRIRADGSSVPIIVLSSRSDEAAKVDALDLGA
                     DDYVTKPFGIDELLARIRAAQRHRLQQTGEAPIFRSGDLTVDLVRRIVTVRGEEVKFS
                     PREYAVLRLLVQHAGKVLTHKMILREVWGGETDVQYLRIYVRALRQKIEADPERPVHI
                     QTETGVGYRLRINDQEP"
     gene            2622..3071
                     /locus_tag="VGC82_07615"
     CDS             2622..3071
                     /locus_tag="VGC82_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY0423174.1"
                     /translation="MEISDFLNPDRVILDLRVQDKAHLISEAARLFCRSVPMLDPNVV
                     EIALAAREQLGSTGLGGGFALPHARIDGLEVYLGMFLRLAKPIEFDAIDAKPVQQVFV
                     LLVPSDATVPHVGALAAITRRFRDGGLVTRLREAKTPATAYGFLTDG"
BASE COUNT          604 a          986 c         1101 g          648 t
ORIGIN      
        1 tccgccatgc taagcgcctg gcggaccagc ttcgcgcgcc gtggaccgca ctcagtgtcg
       61 aaaccgcgcg gccgggcgcg tcgcaaaagg atggcgtccc aaaggacaac gtcgcggagg
      121 cgcttcggct ggcgcaactg cttggcgccg aaccggtgct gatcccgggt caggatgtgg
      181 ccgatacggt gatagactat gctcgcgcca acaatgtcac ccatcttatc gtcggcaagt
      241 cggaccgccc gacctggctg cagttcttgc tggggtcgat cacccagcgc ctcatcaacc
      301 gagcgggtgg catcaatatc catgtgatcg acgtgccggc ggaacccagc gcccgacccg
      361 gcagcgcagc gccagtcgcg ccggcggccg taccaggagc cggcttcgat ccacgggcct
      421 tcgtcatcgc gtcgctgctg gtgggcgtgg cgatccccgt cgccatcggc ctccatcgat
      481 ttctgggcat caccaacatc gcgctggtgg tcctgacggc gatcctgctt gctgccgtgc
      541 tgcatggact gggaccgtcg ctcctcgcat gcttccttgc cgtcgcggca tataatttct
      601 tcttcctgcc gccgttttac actttcacca tcgccgatcc ggaaaacgtc gtcgcgctgt
      661 tcttcttcgc cgttgtagcg gtcgttgtca gcaacctcgc agcccgcgtc cgaggccagg
      721 cgctgtcggc gcggttgcgt gcccgccaaa cggatgatct ataccagttc agccgcaagc
      781 tggccggagc agttgcactg gacgacgtgc tgtgggcgac cgcgcatcag atcgcgatga
      841 tgctgaaggt gcgcgtggtg ctgctgctgc cggacaaaga cagcatcgct gtacgcgccg
      901 gattcccgcc ggacgacatg ttggacgaag cggatctggc agcggccaaa tggtcgtggg
      961 agaagaacca catcgccgga cgcgggtcgg atacgctgcc gggcgggaag tggttgtttc
     1021 agccgctgcg cacaggccgg ggcccgttag gcgttgtcgg catcatacgc gacgaacccg
     1081 ggccgctgct gacgcccgac caggagcggt tgttggacgc gctggcggac caggctgcgc
     1141 tggcgatcga gcgggtcaac cttgctcgcg atctgcatct gtcgcggctg caggtcgaga
     1201 ccgaccggct gcgctctgcc ctgctgacgt cgatctcgca cgatttgcgc acccctttgg
     1261 cttcgatact cggttcggcc accagtctgc ggtcgcagag cctggacaac gcgaccaggg
     1321 acgcgctgct ggctacgatc atcgaggaat cggaccggtt gaaccgtttc atcggcaact
     1381 tgttggacat gacccggctg gagtccggcg ccttgaagcc tcgcaccggc ctgaacgagc
     1441 tatccgacgt gatcggcgca gtgttgcagc gtgccgcgaa aatcctcgct gggcatcgga
     1501 ttgcagtcga tttgccgcgg ggattgccga tgctggcgtt ggacgtcgtg ctgttcgagc
     1561 aagtattgtt caacctgttg gacaacgccg ccaagtatgc gccggccggc agcctgatca
     1621 ccatcgccgc cggccatgac cgcgataccg tcacgctgcg catcctggac gagggcgatg
     1681 gcattccgcc agatgacgtc gaacgcattt tcgagaaatt ctaccggtcc gcgggaggcg
     1741 atcgcaggcg agcgggaacg ggattgggct ggcgatctgc cgtggcttta tcgaagcgat
     1801 ggatggaacg atcaccgccg gtaaccggtc cgaccgcaaa ggggccgtgt tttcgatcgt
     1861 cctgccagtc ccggtcaaca cgctgccggc ggatgaggaa ataatgttat gagcggcgga
     1921 tcggcgccac tgcgggttct ggtggtggat gacgaaccgg cgattcgccg cttcctgcgc
     1981 gccggcctgt ccagccaggg ttatgtggtc agcgagctgg aaatgggtct gcccgccgtc
     2041 gatgtcgctc gacgtaaagg tgccgatctg atcgtgctgg acctgggcct gcctgatatc
     2101 gatggcatcg aagtcatcgg acgaatccga gcggatgggt cgtcggtgcc gatcatcgtg
     2161 ttgtcgagcc gcagcgatga ggcggcgaag gtcgatgctc tcgacctagg cgccgacgat
     2221 tatgtcacaa aaccctttgg catcgacgag cttctggcgc ggatcagggc cgcgcagcgc
     2281 caccgtttgc agcagacggg cgaagcgccg atcttccgca gcggcgacct gacggtcgat
     2341 ctggtccgcc ggatcgtgac ggtgcgcggc gaagaggtga agttctcgcc gcgcgagtac
     2401 gccgtgttgc ggctgttggt gcagcatgcc ggcaaggtac tgacgcacaa gatgatcctg
     2461 cgggaagtat ggggaggcga gaccgacgtg caatacctgc gaatctatgt gcgtgcctta
     2521 cgacagaaaa tcgaagcaga tccggaacgg ccggtccaca tccagacgga aacgggcgtg
     2581 ggctaccggt tgcggatcaa cgaccaggaa ccatgagcgg gatggaaatc tccgactttc
     2641 tgaaccctga tcgggtgata ctcgatctgc gggtacaaga caaagcgcac ctgatcagtg
     2701 aagccgcccg gctgttctgc cgctcggtgc cgatgttgga ccccaacgtg gtggaaatcg
     2761 cgctggcggc gagggagcag ctcggatcga ccgggctggg gggcggattt gcgttgccgc
     2821 acgcacggat cgacggactg gaggtgtacc tgggcatgtt cctccggctg gccaagccga
     2881 tcgaattcga tgcgatcgat gccaagccag tgcaacaggt atttgtgttg ctcgtgccgt
     2941 cggatgctac cgtgccccat gtcggtgcgc tggccgcgat cactcgccgg ttccgcgatg
     3001 gcgggctggt gacgcgtttg cgcgaggcta aaacgccggc aacagcctac ggatttctga
     3061 ctgatggcta gagcagatcc cgttcagata gactatctaa acgggtgaag atgctcgcta
     3121 aacaatgagc tagaccgttg ccagtgagcc taggcgaacg gaaaacggtc taagtgtctg
     3181 tccgggagga tcttgaatat gatcaatagc ttgccacttt ccgggtatgt ggactaagag
     3241 cctgtttgag tgcaattgtg ctcctccgtc cgcatcttaa tctcagtgat tgaagaagag
     3301 gtccgcctgg cagggcggtg agactggatg tggacaaag
//
LOCUS       DASWQN010000465         5105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_968039, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000465 DASWQN010000000
VERSION     DASWQN010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968039"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..3184
                     /gene="gyrA"
                     /locus_tag="VGC82_07620"
     CDS             377..3184
                     /gene="gyrA"
                     /locus_tag="VGC82_07620"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY0423175.1"
                     /translation="MHPVTLEEEMRRSYLDYAMSVIVSRALPDARDGLKPVHRRILYG
                     MHEAGYTPDKAYRKSARVVGDVMGKYHPHGDSSIYDAMVRMAQSFSMRLPLIDGQGNF
                     GSVDGDNPAAMRYTEVRLAKAAALLLEDIDKDTVTFQPNYDESESEPLVLPAKFPNLL
                     INGANGIAVGMATNIPPHNPGEIIDATLALIRDPDISLDDLMKIVPGPDFPTGGIIIG
                     RAGIRQAFETGRESIVIRAKTEFEEIRKDRQAIIVTEIPYQVNKKTLIEKIAELVRGK
                     VIEGIGEMRDESDRSGMRIVMELKRDATQEVVLNQLFRFTQLQTSFGINILALDDGQP
                     KQMGVREALMCFIRFREDVILRRARFELGKARDRAHNLVGLAIAVANIDEVIRLIRAS
                     PDAAAARVALMGRDWPAEDIGALLALIDEPGNVIADDGTVRLTEEQARGILELRLQRL
                     TGLEREKIQAEMTDVAGKIRELLEILGSRIRRMELMTEELTEARKEIATPRMTDIADY
                     VGDQDDESLIEPGQMVVTITRDGFIKRTSLETFRAQNRGGRGRSGAGTRGDDIVTRSF
                     NGHTHQWVLFFSSGGKAFRVKVWKLPEAGPTAKGRALVNLLPELGADTITTVLPLPQD
                     ETLWESLHLVFATALGNVRRNRLSDFRNVRAAGLIAMKLDEGDHLVGVATCREGDDVL
                     LATRKGRCIRFVLSDEQVRVFAGRDSSGVRGIRLLSGDEVISLSVLRHVQASPEDRIA
                     YLKQANAKRRGNGEADAEAPVEIDGEEFVPEVTLSPERFADMETAEEMLLTVTDMGFG
                     KRTSAYEYRVTGRGGQGIGNITLAPRNGKTVVATFPVRQGDDIMMVTDAGRLIRVPVD
                     QVRTTGRQAMGVTLFRVDKGEHVTSVFTVLEEESEDNGATEELGPNQDGPHQDGPNQD
                     GLGDSASDDNDKPAPEEGNDG"
     gene            3177..3698
                     /gene="coaD"
                     /locus_tag="VGC82_07625"
     CDS             3177..3698
                     /gene="coaD"
                     /locus_tag="VGC82_07625"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY0423176.1"
                     /translation="MADKAADHPQRVGLYPGTFDPITNGHLDVIARAARLLDKLVVGV
                     AINTGKGPLFTIEERVELVSAEIESIAARNGMVIEVIPFDTLLIDFARRIGASMIVRG
                     LRAVSDFDYEFQMAGMNYRMAPDVETVFLMASERHQFIASRLVKEVAMLGGDITSFVP
                     RLTLERILQRIRS"
     gene            3759..4310
                     /locus_tag="VGC82_07630"
     CDS             3759..4310
                     /locus_tag="VGC82_07630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0423177.1"
                     /translation="MHRRTLIAATAAAAVSARALAQSNELEDTIYLDLKQGRVVILLL
                     PDVAPLHVERIKTLSRQGFYDGTPFHRVMEGFMAQGGDPTGTGTGGSKLPNLPPEFSQ
                     RHFVRGVCGMARSSAPDSANSQFFIMFAPAPSLDGKYTIWGQVTRGMEYVDQIKRGAG
                     SGGMVPGEPDRIVHMRVAADVKQ"
     gene            4330..4830
                     /locus_tag="VGC82_07635"
     CDS             4330..4830
                     /locus_tag="VGC82_07635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0423178.1"
                     /translation="MSDSKPALDPENTLYLDLKQGRVVIQLLPDIAPMHVQQIKTLVR
                     KGFYDGTVFHRVIEGFMAQGGDPTGTGTGGSDLDNIRAEFSDKAKFVRGACGMARSQS
                     PNSANSQFFIMFEPASHLNGQYTIWGQVVEGMDLVDQIKRGSGGGGTVSDPDKIEKMT
                     VAADAS"
     gene            4942..5017
                     /locus_tag="VGC82_07640"
     tRNA            4942..5017
                     /locus_tag="VGC82_07640"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4975..4977,aa:Lys,seq:ttt)
BASE COUNT         1018 a         1545 c         1664 g          878 t
ORIGIN      
        1 cagattggtg tcgagcgcga ctggcgggag gtccttcagg ttggcgacca gcttcgcgat
       61 cgacgggtcg ccggtaaagc ggtgttccgt gtgggaacgc ggtgatcccg agcgttccac
      121 gacgcccaag cttgccatga cgccaagctt cccacgaccc ggatgccaag ccgccacaag
      181 ccagtgcgtc gtcgcctggc tgggctggca ctgagcggca aactgcctct cgccattggt
      241 gtcaaatcag cctatctaac gccaataaat gcgcgtctga cctgatccct ggttgggcgg
      301 gcgcgagcaa cggattgagc cactcttgag cgacacacct gaccagccgc tggagccgac
      361 cggcccctac ggtgaaatgc acccggtaac tttagaagag gagatgcgcc gctcctacct
      421 cgattacgcc atgagcgtga tcgtctcgcg cgcattgccg gacgcccgcg acgggctgaa
      481 gccggtgcat cgacgtatcc tttacgggat gcacgaggct ggttacacgc cggacaaggc
      541 gtatcggaag tccgcccgtg tggttggcga cgtgatgggt aaatatcacc cccacggcga
      601 cagctcgatc tacgacgcca tggtgcgcat ggcgcaaagc ttctccatgc ggctgccgct
      661 gatcgacggg cagggcaatt tcggttccgt ggacggcgat aacccggcgg cgatgcgtta
      721 taccgaggtc cggctggcca aggccgcggc cctgctgctg gaagatatcg acaaggacac
      781 cgtcaccttc cagccgaact acgacgagtc ggaaagtgag ccgctcgtgc tgccggccaa
      841 gttcccaaat ctcctgatca acggcgccaa cggcatcgca gtcggcatgg cgaccaatat
      901 tccaccgcac aaccccggcg agatcatcga cgccacgctg gcgctgatcc gcgatccgga
      961 catctcgctg gacgatctga tgaagatcgt ccccggtccg gacttcccga ccggcggcat
     1021 catcatcggc cgcgccggca tccgccaggc gttcgagacc ggccgagaaa gcatcgtcat
     1081 tcgcgccaag acggaattcg aggaaatccg caaggaccgg caagcgatca tcgtgaccga
     1141 aattccctac caggtgaaca agaagaccct gatcgagaag atcgccgaac tggtgcgcgg
     1201 caaggtcatc gaaggcatcg gcgaaatgcg cgacgagagc gaccgctccg gcatgcgcat
     1261 cgtcatggaa ctgaagcgcg acgcgacgca ggaagtcgtg ctcaatcagc tattccgctt
     1321 tacccagttg cagaccagct tcggcatcaa catcctggcg ctggacgatg gccagcctaa
     1381 gcaaatggga gtgcgtgagg cgctgatgtg cttcatccgc ttccgcgagg acgtcatcct
     1441 ccggcgtgcc cgtttcgaac tcggcaaggc gcgcgatcgg gcgcacaacc tggtcggcct
     1501 ggcgatcgcg gttgccaata tcgatgaagt gatccggctg atccgcgcca gtccggacgc
     1561 cgccgccgcg cgggtcgccc taatgggccg agactggccg gccgaggata tcggcgcgct
     1621 gctggcactg atcgacgaac ccggcaacgt catcgccgac gacggcacgg tgcggctgac
     1681 cgaggaacag gcgcgcggca tcctggaact ccgcctgcag cgcctgactg gcctggaacg
     1741 cgaaaagatc caggccgaga tgaccgatgt ggctggcaag atccgtgaac tgctggagat
     1801 cctggggtcg cgcatccggc gcatggaatt gatgaccgag gaactgactg aggctcggaa
     1861 ggagatcgcg actccgcgga tgaccgacat tgccgactat gtcggcgacc aggacgacga
     1921 aagcctgatc gagccaggcc agatggtggt gaccatcacc cgcgacggct tcatcaagcg
     1981 cacctcgctg gaaacattcc gtgcccagaa tcgcggcggg cgtgggcgaa gcggcgccgg
     2041 cacgcgtggc gacgacatcg tcacccgcag cttcaatggc catacgcacc agtgggtgct
     2101 gttcttctcg tccggcggca aggcttttcg ggtgaaggtc tggaaactgc ctgaggcggg
     2161 gccgacggcg aaaggccggg cactggtgaa tctgttgcct gaactggggg cggatacgat
     2221 cacgacggtg ctgccgctgc cgcaggacga aaccctgtgg gaaagcctgc acctggtgtt
     2281 cgcgaccgca ttgggcaacg tgcggcgcaa ccgcttgtcg gacttcagga atgtccgcgc
     2341 ggcgggcctg atcgcgatga agttggacga aggcgatcac ctggttgggg tggcgacctg
     2401 ccgtgaaggc gatgacgtgc tgttggcgac ccgcaagggc cgctgcatcc gtttcgtgct
     2461 gtcggacgag caggtacggg tatttgccgg gcgagacagc tccggcgtgc gaggcatcag
     2521 attgctgtct ggcgacgagg tcatcagcct gtcggtgcta cgccacgtac aggcctcgcc
     2581 ggaagaccgg atcgcttacc tgaagcaggc taacgccaag cggcgtggca atggcgaggc
     2641 ggacgccgaa gcaccggtcg agatcgacgg cgaggaattt gtccctgagg ttacgctttc
     2701 gccggagcgc ttcgccgaca tggagaccgc cgaggagatg ctgctgacgg tcaccgacat
     2761 gggcttcggc aagcggacct cggcctacga gtaccgggtt acggggcgtg ggggccaggg
     2821 catcggcaac atcactttgg ctccccgcaa tggcaagacc gttgtggcaa ccttcccagt
     2881 acgtcagggc gatgacatta tgatggtcac cgatgccgga cgcctgatcc gcgtgccggt
     2941 cgatcaagtg cgaacgactg gacgtcaggc gatgggcgtg accctgttcc gggtggataa
     3001 aggcgagcat gtgaccagcg tcttcacggt gctggaagaa gaatccgaag acaacggcgc
     3061 tacggaagaa cttggcccga atcaagatgg cccgcatcag gatgggccga atcaggatgg
     3121 actaggtgat agcgcgtcgg acgacaacga taagcctgct cccgaggaag gcaacgatgg
     3181 ctgacaaggc tgctgatcat ccgcaacggg tcgggctcta tccagggacg ttcgacccca
     3241 tcaccaacgg tcatcttgat gtcatcgccc gcgctgcccg gttgctggac aagctcgtcg
     3301 tcggcgttgc gatcaacacc ggtaaggggc cgttgttcac gatcgaggaa cgcgtcgaac
     3361 tcgtgagtgc cgagatcgag tcgattgctg cgcggaacgg catggtcata gaagtcattc
     3421 ctttcgacac gctgctgatc gatttcgcgc gccggatcgg cgccagcatg atcgtccgcg
     3481 gattgcgggc ggtctcggat ttcgactacg agtttcagat ggccggaatg aactaccgca
     3541 tggccccgga cgttgagacg gtgttcctga tggccagcga aaggcaccag ttcatcgcgt
     3601 cccgtctggt caaggaagtt gcgatgctcg gcggcgatat cacgtcgttc gtgccgcggt
     3661 tgaccctgga acgcatcctg cagcgtatcc ggagctgagc cggcgacctg attgattgga
     3721 cctgccgaaa caattgacct gaatgaagga ggcgttccat gcatcgccgc accctgatcg
     3781 ccgcaaccgc cgccgcggca gtttccgcgc gcgcgctggc gcagtcgaac gagctggaag
     3841 acacgattta tctggacctg aagcagggac gggtcgtgat cctgctgctg ccggacgtgg
     3901 cgccgctgca cgtcgagcgg atcaaaacgc tgtcacgtca gggcttctac gacggcacgc
     3961 cgttccaccg ggtgatggaa gggttcatgg cgcagggtgg cgatccgacc ggcaccggca
     4021 ccggtggcag caagctgccg aacctgccgc cggagttcag ccagcgccac ttcgtgcgcg
     4081 gcgtgtgcgg catggctcgc agcagcgcgc cagacagcgc caacagccag ttcttcatca
     4141 tgttcgctcc ggcaccttcc ttggatggca aatacacgat ctggggccag gtcacgcgcg
     4201 gcatggagta cgtcgatcag atcaagcgcg gcgccggctc gggcgggatg gttccgggcg
     4261 agccggatcg catcgtccac atgcgcgtcg ccgcggacgt caaacagtaa cgcacaggag
     4321 acaggaacca tgagcgattc gaagccagct ctcgatcccg agaacacgct gtatctggac
     4381 ctgaagcagg gccgggtggt gatccagctt ttgccggaca tcgcgcccat gcatgtccag
     4441 cagatcaaga ccttggtgcg taaagggttc tatgacggca cggtgttcca ccgcgtcatc
     4501 gaaggcttca tggcgcaggg cggcgacccg actggcacgg gcaccggcgg aagcgatctg
     4561 gacaacatcc gggcggaatt ttccgacaag gcgaagttcg tgcgcggggc atgcggcatg
     4621 gcgcgttcac aaagcccgaa tagtgccaat agccagttct tcatcatgtt cgagccggcg
     4681 tctcacctga atggccagta cacgatctgg ggccaggtcg tggagggaat ggacctggtg
     4741 gaccagatca aacgcggcag cggcggcggt ggcaccgtgt ccgatccgga caagatcgag
     4801 aagatgacgg tagcggccga cgccagttag caagaggcgt atgattcccg caccgcgata
     4861 cctgcttgat cgaccgcaag gctgatacac agcctggacc gcttgaccgg cggcccggca
     4921 tgtcgttcaa agcgccccgg cgagcctgta gctcagttgg tagagcacga gacttttaat
     4981 cttggggtcg tgggttcgat tcccaccggg ctcaccaagc cccctgccgt gttacccagt
     5041 aaaggaaatc gcgcgcactg ggactaaacc accgtgccgc tctttcctgc ccgccgctgt
     5101 ccggg
//
LOCUS       DASWQN010000466         3663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2287991, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000466 DASWQN010000000
VERSION     DASWQN010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2287991"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1256)
                     /locus_tag="VGC82_07645"
     CDS             complement(45..1256)
                     /locus_tag="VGC82_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0423179.1"
                     /translation="MTMATGQRGTIRRRDIMLGVGMAGVASGMPMPAIAQNKPIRMGW
                     IAAVTGAFASNAQAQDWGFHTMVQDLNDAGGVNGRMLEIVMRDSAGDPSKAVSFAKEL
                     VYNEKVDVVCGPINSGEVLPTLGVISGGKTLQMVGGVIDELIDPVKYPLVFRNLNTNT
                     QYIKIATRFCLEKLNRKKIAIIDDNTGYGVLAMQGLVRMLDGLGLKPAFTVTVDPAKT
                     DVTDEILKARSSGADVIQTWTNATGFMARVLNARGEQQWDVPIVGNPSMYQDQVGALL
                     SKPAYWANAFAAGYANSVTDANGKLPPATQAFLDKHKEGAAPYIKTAVFAIMQGATSA
                     LIPVTGWRKAGTTEPFATKAALETIPVIETPYGRFSYSPTDHNGFGDDGMAICIANSR
                     QPDGSLKAIPL"
     gene            complement(1339..2391)
                     /locus_tag="VGC82_07650"
     CDS             complement(1339..2391)
                     /locus_tag="VGC82_07650"
                     /inference="COORDINATES: protein motif:HMM:NF005757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0423180.1"
                     /translation="MISNSYYSQDLHGPYELHDIGNLELEEGGTIRGCQLAYTTFGSL
                     DAAGDNAILVPTWYSGTSKIIEQVYIGQGRALDPDKYFIVVVNQIGNGLSTSPHNTPA
                     PAGMGNFPHVRIGDDVRAQHRLVTEKLGIQRLALVVGGSMGAQQTYEWAVRYPDMVKR
                     AAPIAGTARNTAHDFLFTETLVEAITSDPGYKKGFYAAPADVREGLLRHAKMWAVMGW
                     STEFFRQDRHKTLGFSSMDDFIINFMFGYFSVMDPNNLLCQAWKWQRGDVSRHTGGDL
                     RSALGRIKAKTYVIPMSSDMFFPPADCQAEWRLIPDAEFRPIQTMDGHLALFGADADA
                     IGQLDRHLKELLATKA"
     gene            complement(2431..>3663)
                     /locus_tag="VGC82_07655"
     CDS             complement(2431..>3663)
                     /locus_tag="VGC82_07655"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0423181.1"
                     /translation="KGEDVTARYLIAAVGSLSITNIPRFKGLETFKGKWYHTSQFPHS
                     GVDFTAKRVAVVGTGATAVQAIPEIAQQAKQLTVFQRTANYCVPARNGKVDPEVTAAR
                     KADYDGVVERIRESFFGFEYDFIPKSVLEASPEEREREFDRMWDTGGFAFWLANYQDM
                     FFSQEANDLCADYIKRKIRKTVKDPAVAERLIPKGYAYGTKRQPLDTNYYETFNKDNV
                     LLVDANTDGAIEEITQKGIRAGGREYEFDIIVFATGFDAMTGPLKALNLKGRGGRTLD
                     EQWIDGPHSYLGISVAGFPNLFTITGPQSPSVLSNMPVSIEQHVDWVTDCIDTMRKTG
                     KTCIEATSEAQEQWVAHVNEIVSATLMTGANSWYMSANIPGKPRAFLPYLGPQGVGGY
                     RKKCDEVAAKGYEGFALA"
BASE COUNT          685 a         1134 c         1057 g          787 t
ORIGIN      
        1 ccctgcgtcc gccgcatgat gcgacgaact tcgggggcac gacactacaa agggatcgct
       61 ttcaggctgc cgtcaggttg ccgcgagttg gcgatacaga tcgccatgcc gtcgtcgccg
      121 aatccattgt gatcggtcgg cgagtaggag aaccggccat agggcgtctc gatgaccgga
      181 atcgtctcca gggcggcctt ggttgcaaac ggttcggttg tcccggcctt tcgccagccg
      241 gtgacgggga tcagcgccga ggtcgctccc tgcatgatcg cgaagaccgc ggtcttgata
      301 tagggcgccg cgccttcctt atgcttgtcg aggaatgcct gggtagcggg cggcaacttg
      361 ccgttggcat cggtgacgct gttcgcgtag ccggcggcaa aggcgttggc ccagtaggcc
      421 ggcttgctca gcagcgcacc cacctgatcc tgatacatgc tggggttgcc gacgatgggg
      481 acatcccatt gctgctcgcc gcgcgcgttc agtacgcgcg ccatgaagcc ggtggcattg
      541 gtccaggtct ggatgacgtc ggcccccgag gagcgggcct tcaaaatctc atccgtcacg
      601 tcggtcttgg ctggatcgac ggtgaccgtg aaagccggct tcaggcccag gccatccaac
      661 atcctcacca ggccctgcat cgccagcacg ccgtaaccgg tattgtcgtc gatgatcgcg
      721 atctttttcc ggttcaattt ctcaaggcag aaccgtgtgg cgatcttgat gtactgcgtg
      781 ttggtgttga ggttgcggaa gaccagcgga tatttcaccg gatcaatcag ttcgtcgatg
      841 acgccaccca ccatctgcaa cgtcttgccg ccggaaatga ccccaagcgt gggcagcacc
      901 tcgccactgt tgatggggcc gcacacgaca tcgacttttt cgttgtaaac cagttccttt
      961 gcaaagctca ccgccttcga tggatcaccc gccgaatcgc gcatgacgat ttccagcatg
     1021 cgtccgttga cgccgccggc gtcgttgagg tcctgaacca tggtgtggaa gccccagtcc
     1081 tgcgcctggg cgttgctggc gaacgcgccc gtgaccgcgg cgatccagcc catgcggatg
     1141 ggcttgttct gcgcgatggc gggcatcggc atcccggaag caacgccggc catcccaacg
     1201 cccaacatga tgtcacgtct tcggatcgtg cctcgctgcc ccgtcgccat ggtcatgacc
     1261 ccccgttatc atttttgttt cttgaggtta tgcaagatta gattggctgt caaaatggcc
     1321 gaagccccgg cgggtgcctc aagctttcgt cgcgagcagt tccttcaggt gccggtcgag
     1381 ttggccaatc gcatcggcat cggcgccgaa cagcgcgaga tgtccatcca tcgtctggat
     1441 cggccggaat tcggcgtcgg gaatgagccg ccactcggcc tggcagtccg ccggagggaa
     1501 gaacatgtcc gaagacatcg ggatcacata cgtctttgcc ttgatgcggc ccaacgccga
     1561 gcgcaggtcg ccgccggtgt ggcgactgac gtcaccgcgc tgccacttcc atgcctgaca
     1621 caacaggtta ttggggtcca ttacgctgaa atatccaaac atgaagttga tgatgaaatc
     1681 atccatcgaa gaaaagccca gcgtcttgtg ccgatcctgg cggaaaaact ccgtgctcca
     1741 acccatcacc gcccacatct tggcgtggcg cagcaaacct tcccgaacgt ccgccggcgc
     1801 cgcataaaat ccctttttgt atccgggatc cgatgtaatc gcctcgacca gcgtttcggt
     1861 aaacaggaaa tcatgggcgg tgtttctcgc ggtcccggca attggcgccg cgcgcttcac
     1921 catgtccgga taacgaaccg cccattcata agtctgctgc gcccccatcg acccgccgac
     1981 aaccagggcg agccgctgaa ttccgagttt ttccgtcaca agcctgtgct gcgcccgcac
     2041 gtcgtcgccg atgcgcacat gggggaagtt gcccatcccg gccggcgccg gcgtattgtg
     2101 cggcgaggtt gaaagaccat tgccgatctg attgaccacg acaatgaaat acttgtcagg
     2161 gtcgagcgct cgaccctgac cgatataaac ctgctcgatg atcttgctgg tgccggagta
     2221 ccaggtcggc accaggatag cattgtcgcc ggcggcatca agcgaaccga acgtcgtata
     2281 agcgagttgg cagccccgga tcgtgccgcc ctcttcgagt tcgagattgc cgatatcgtg
     2341 caactcgtaa gggccgtgca ggtcctgcga gtaatagctg ttgctgatca ttattccccc
     2401 gggacctgtt cggccttctg ccgattctcg ctatgccagt gcaaacccct cgtagccctt
     2461 ggccgcgacc tcgtcacact tcttccggta gccaccgacg ccttgtggac ccagatacgg
     2521 caagaaggcg cggggcttgc ccggaatgtt tgcactcatg taccatgagt tcgcaccggt
     2581 catcagcgtg gcgctgacga tctcgttgac atgggcgacc cactgttcct gcgcttcaga
     2641 cgtcgcctcg atgcaggtct tgccggtctt gcgcatggta tcgatgcaat cggtgaccca
     2701 gtccacatgc tgttcgatcg acaccggcat gttcgacagc accgacgggc tttgcgggcc
     2761 ggtgatcgtg aacaggttcg gaaagcccgc gaccgagata ccgagataag agtgcggacc
     2821 atcgatccat tgctcatcaa gggtgcgacc gccgcggccc ttcaggttca aggctttcag
     2881 cggcccggtc atcgcatcga aaccagtggc gaaaacgatg atatcgaact cgtattcccg
     2941 tccgcccgct cggattccct tctgggtgat ttcctcgata gcgccatcgg tgttggcgtc
     3001 caccagcaac acattgtcct tgttgaaggt ctcgtagtag ttggtgtcca atggttggcg
     3061 cttggtgccg taagcataac ccttgggtat caacctttcg gctacggccg gatctttcac
     3121 cgtcttgcgg atcttccgct tgatgtagtc cgcacacaga tcattcgctt cctgactgaa
     3181 gaacatgtcc tgatagttgg ccagccagaa ggcgaagcca cccgtgtccc acatcctgtc
     3241 gaattcccgc tcgcgctctt ccggcgaggc ctccagaacc gactttggaa tgaaatcata
     3301 ctcgaagcca aagaacgatt cgcggatacg ctcgaccacg ccgtcatagt ccgctttccg
     3361 agctgcggtg acttcgggat ccaccttgcc gttccgcgcc ggcacacaat aattggcggt
     3421 tcgttggaac acggtaagct gtttggcctg ttgcgcgatt tcggggatgg cctgcaccgc
     3481 ggtggcgccc gtgccgacca cggccacccg cttggcagtg aaatcgacgc ccgaatgcgg
     3541 gaattgactg gtgtggtacc acttcccctt gaatgtctcc aggcccttga accggggaat
     3601 gttggtgatg gacaaactcc ccacggccgc gatcaggtat cgcgcggtga cgtcttcgcc
     3661 ctt
//
LOCUS       DASWQN010000467         3068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_440729, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000467 DASWQN010000000
VERSION     DASWQN010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440729"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="VGC82_07660"
     CDS             <1..1481
                     /locus_tag="VGC82_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEY0423182.1"
                     /translation="ARGAQLIKGELEVGGTVQPLRQGGWHQIGGSAVLRAGAHGFTWL
                     RDLRALGTDAARLRARALVSEWIANPPSDPVAHRPDVTGARITAWLGHYDFFAATADD
                     VFRQRLMSRLVADARGLSAALPAEELDARALTAMKGLVAAAVALREHAGFLTRALRFL
                     PQEISRQILPDGCHAERSPAAQLSALQDLTEIRALFQTSQTQPPPVLAHAIERMAPAL
                     RMMRHGDGGLALFNGSKEETSTLVDLVLTQAGRGGRGPSALTEGGFSRMQAGRSVLIV
                     DCGAPAPPGLDRLAHAGTLSMELSVGRDRVIVNCGSFLAGPPEWHDAARATAAHSTLV
                     IGDLNSSELKPEGLGRRPNMVEVNRQEAAGAHWLDVSHDGWRRTFNAVHRRRLYMAES
                     GEDIRGEDLIESPDPQPFTLRFHLHPDVQANVQQDGAAVLLRLKSGTFWRLRADGAQL
                     NLEETIYLGGNEPRRGEQIVLTGFADGPQHIKWALSKVERAG"
     gene            1521..2627
                     /locus_tag="VGC82_07665"
     CDS             1521..2627
                     /locus_tag="VGC82_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0423183.1"
                     /translation="MKIIEITNVDFSLRHFLLPLMRAIRARGHEVVGACAEGPLLNDV
                     RAEGFRVVAIPFVRRLSPVGQLRAFQSLVAILRAEKPDLVHAHMPISGFLARLAAWIA
                     GVPTVAYTCHGFLFNHAASSWPRRSLSFVMEWIAARVTDVFLTVSEAEALDARRLHIA
                     RHAQPVRNGRDPKVFLPCPSARQRIRTELGVPDDRVVIIAVSRLVWHKGYPELAAAMR
                     SVPEAELWVVGKRLDSDRGADMALLLRSAGLGPRLRMLGYRTDIPALLAGADIFTLPS
                     RFEGLPMSVIEAMLTGLPVVATNVRGPAEQVVDGVTGLQVPAGDPAALGLALSRLARD
                     SALRARMGLAGRQRALDCYDEAKVLSRTLDLLGL"
     gene            2668..>3068
                     /gene="purH"
                     /locus_tag="VGC82_07670"
     CDS             2668..>3068
                     /gene="purH"
                     /locus_tag="VGC82_07670"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502247.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY0423184.1"
                     /translation="MTDILPIRRALISVSDKSGLVPLAQALTGHGAEILSTGGSAKTL
                     REAGVPVKEVSEHTGFPEIMDGRVKTLVPQIHGGILGRRDVPEHMEQMRLHAIAPIDL
                     VAVNLYPFETTVANGAAYEECVENIDIGGPAL"
BASE COUNT          471 a         1028 c         1018 g          551 t
ORIGIN      
        1 ccgcacgcgg tgcgcaactg atcaagggcg agctagaggt cggtggcacg gtgcagccgc
       61 tgcggcaagg cggctggcac cagatcggcg gatcggccgt gctgcgcgcc ggtgctcatg
      121 gcttcacctg gctgcgtgac ctccgcgccc tgggcaccga cgccgcccgg ttgcgggccc
      181 gcgccctggt gtcggaatgg atcgccaacc cgccgtccga ccccgtcgcc caccgccccg
      241 acgtcaccgg tgcccgcatc accgcctggc tcgggcacta cgacttcttc gcggcgaccg
      301 ccgatgatgt tttccgccag cggcttatga gtcgtctggt cgccgacgcc cgaggcctgt
      361 ccgcggcgct gccggccgag gaactggatg cccgcgccct gaccgcaatg aaaggcttgg
      421 ttgccgccgc cgtcgcattg cgcgaacatg caggcttcct gacgcgggcg ttgcgtttcc
      481 tgccgcagga gatcagccgc cagattctgc ccgatggctg ccacgccgaa cgcagccccg
      541 cggcgcaact gtctgccttg caggacctca cggaaattcg ggcactgttt cagacctcac
      601 agacccagcc gccgccggtt ctggcgcatg cgatcgagcg gatggccccg gcgctgcgca
      661 tgatgcgaca cggcgacggc ggtctggctt tgttcaacgg cagcaaggaa gagacctcca
      721 cgctcgtcga tctggtgctg acccaggcgg gccgaggcgg acgcggccct tcggcgctga
      781 ccgagggcgg gttcagccgg atgcaggccg gacggagtgt gctgatcgtc gattgcggtg
      841 cgccggctcc gccagggttg gaccggctgg ctcatgccgg cacgctgtcg atggagcttt
      901 cggtcggccg cgaccgggtg attgtaaatt gcggctcgtt tcttgccggc ccgccggagt
      961 ggcatgacgc cgcacgcgcc accgccgccc actcgacact ggttatcggc gacctgaaca
     1021 gcagcgaact gaagcccgaa ggccttggac gccggccgaa tatggtggaa gtaaaccgcc
     1081 aggaagccgc cggcgcgcac tggctggatg tgtcgcatga cggctggcgg cggaccttca
     1141 acgccgtcca ccgccgccgg ctgtacatgg ccgaaagcgg cgaggatatc cgcggcgagg
     1201 atctgatcga atcgcccgat ccccaaccgt tcacgctgcg gtttcatctc caccccgatg
     1261 tgcaggccaa cgtgcaacag gatggggccg cggttctgct acggctaaaa tccggcactt
     1321 tctggcgact gcgagccgat ggtgcgcagt tgaacctgga ggaaaccatc tatttgggcg
     1381 gtaatgaacc ccggcgaggt gagcagatcg tgctgaccgg ttttgccgat gggccgcagc
     1441 acatcaagtg ggcactcagc aaggttgagc gcgccgggta gggccggcgg tagcagcctc
     1501 ttcaccggcg cgccccaacg atgaagatca tcgagattac caacgtggat ttctcgttgc
     1561 ggcacttcct gctgccgctg atgcgcgcaa tccgggcccg cggccacgag gtcgttggcg
     1621 cctgcgccga aggtcccttg ttgaacgacg tccgggccga gggcttccgc gtcgtcgcca
     1681 tccccttcgt ccgccgcttg tcccctgttg ggcagctccg cgccttccag tcccttgtcg
     1741 ccatcctgcg ggcggagaag cccgaccttg tccatgccca catgccgatc agcggattcc
     1801 ttgcacgcct tgcggcgtgg atcgccggcg tcccaacggt cgcctatacc tgccatggct
     1861 tcctgttcaa tcacgccgct tcctcgtggc cgcgccgcag cctgtccttt gttatggagt
     1921 ggatcgcggc acgggtgacc gacgtgttcc tgactgtctc ggaagccgag gctctcgatg
     1981 cccgccgtct gcacatcgcc cgccacgccc aaccagtccg caatggtcgc gacccgaagg
     2041 ttttcctccc ctgcccttcg gcccggcagc gcatccgaac cgaactcggc gtacccgatg
     2101 accgcgtggt gatcatcgcg gtatcgcggc tggtctggca caagggctat cctgaactcg
     2161 ccgcggcgat gcggtcggtg ccggaggccg agctttgggt ggtgggcaaa aggttggaca
     2221 gcgatcgggg cgctgatatg gccctgctgc tacgctccgc tggcctgggg ccccgcctgc
     2281 gtatgctggg ctatcgcact gacatcccgg ccctgctggc tggtgccgac atcttcaccc
     2341 tgcccagccg ttttgaaggc ttgccgatgt cggtgatcga ggcgatgctg accggcctcc
     2401 ccgtcgtcgc caccaacgtt cgtggtccgg cggagcaagt ggtggacggc gttaccggtc
     2461 ttcaggttcc ggctggcgat ccggcggcgc tcgggttggc tttatccagg ctggcccgcg
     2521 attccgctct gcgcgcccgg atggggttgg ctggccggca gcgggcgctg gactgctacg
     2581 acgaagcgaa agtcctttcg cggaccctgg acctgctggg cctgtaggtt tatccgcgcg
     2641 atcgcagcat aacggaacac cgacgccatg accgacatcc tccccatccg ccgggcgttg
     2701 atttccgtct ccgacaagtc cggattggtg ccgcttgcgc aggcgttgac gggacatgga
     2761 gcggaaatcc tatccactgg cggatcggcc aagaccctgc gtgaggccgg cgtgccggta
     2821 aaagaggtgt ccgagcatac cggcttcccg gagatcatgg acggccgcgt caagacgctg
     2881 gtgccgcaga tccatggcgg catcctgggg cggcgggatg ttccagagca catggaacag
     2941 atgcggctgc acgcgatcgc gccgatcgat cttgtggcgg tgaacctgta cccgttcgag
     3001 acaaccgtcg ccaacggagc ggcgtatgag gaatgcgtgg agaacatcga catcggcggc
     3061 ccggcgct
//
LOCUS       DASWQN010000468         7654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2288112, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000468 DASWQN010000000
VERSION     DASWQN010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7654
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2288112"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3304
                     /locus_tag="VGC82_07675"
     CDS             <1..3304
                     /locus_tag="VGC82_07675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0423185.1"
                     /translation="HTYEAVAEDFRTWRPKLSDRAVVLFHDTNVRRDDFGVWRLWREL
                     SAEYPSFEFLHGHGLGVIGVGENLPAAIRALFDCDEHDDRRTAVRRLFAARGLAVQRT
                     YDLGSTSAHAAALSGDVMSLNNETAALREQVATLREEIETVRAAATTYQAAAEALQAD
                     VVSLQADAASRTAAIDSLRDELAAKSAKAAILQGQIDVRAAEVAELTARLVNEVAEAR
                     QEAALSKRASEAAADDAMAQAMARLAAAEARCAALLASTCWRMTAPVRAVKTAILELR
                     AQDPAKRNRMTRWTSRLTRRLPDRDAPDAVLGAPVAAASRDRIMPPAWHVGLLRPRVL
                     IVAELSLPQCKKYRVDQKHDMIEALGWDCTVVAWQQTDRVRTLIQTHSVVIFYRVPAF
                     PEVLPIIQEADCLGLPHYFELDDLIFDLDDYAANSNLIGLDPATTRGLFDGARLYRRM
                     LELCRNVIASTPYLQERMHAVGGGSSAVIENALDAETLHAATTARPRRPDGKIRIIYG
                     SGTRTHDADFAMIADALKRVITENGDVEIVLVGDLNPPPALLEFNDRLRRLPACPYPE
                     YLALVADSDIAIAPLIAGNFNDAKSNIKFLEAASVGVPSVCSPRSAFAQAIRHGETGF
                     LAATGDDWYAALTSLITDDALRHRVGQAARQSVLECYSPEAIGRRQVKPLLSKHQPAG
                     DSRLHILQVNILCSPQTFGGATVVMEEMTQHLNNRADTRVSIVTSAPAGWIKPYALER
                     GEVLGVPVFRIGIQDNAGWMQAFWDEEAGEAFREALRALRPDVVHFHAIQGLGAACLT
                     ACRDEGIPFVVTLHDAWWVCERQFLVSGEGKYCGQKRIDANVCATCVPDIGVTVLRNL
                     RLRQALESADLLLTPSQHWRRFYVDNGFPPDRVVVNENGIPLPADPQPNEGPARLRFG
                     FVAGAESVKGFPLLKRTFEALDRDDWELVLVNHGLSMGLDSYAGVEWNARGSVRTVPP
                     FNTEERDSFYASIDVLLFPSQWPESFGLTVREALVRNKWVIATGQGGAAEAIKPGVNG
                     TLIPIGDDIAPFREAIEDLLDRKPDLAGWINPFAGRTRGFAAQAAELRGILGAVASSG
                     HSQPADVVAAVA"
     gene            3379..4455
                     /locus_tag="VGC82_07680"
     CDS             3379..4455
                     /locus_tag="VGC82_07680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0423186.1"
                     /translation="MEDRPAELLEGIDRRSKILELGPSVSPMAPRSAGWDVTVVDHAT
                     QEELVEKYKVDPNVTVANIEKVDYVWSNGPLHEAVPPELHGTFDVLIASHVIEHLPNP
                     IGFLQSAARLLRPGTGVVALAVPDKRWCFDYFKQISSTGDFFAAHRVGAQRHRLATRF
                     DHSAYIAFDNGRGGWGREPLTGLKLVDSLEHAYSEFQSWTDDPSAPYVDCHAWHFTPA
                     SFELLILEAGAVGLLDWRLAWLHPRAGVEFFARLVPGHEQFATVADRESRRLELLKQI
                     LLDVREQTDWLVEPRPGISQAVAPDISQTVAPDIGFDANAREQLRQIAETAAEVRAAL
                     RPGRAVWQGMLPLRRQIARLRGKS"
     gene            complement(4522..5451)
                     /locus_tag="VGC82_07685"
     CDS             complement(4522..5451)
                     /locus_tag="VGC82_07685"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY0423187.1"
                     /translation="MRGVIFVVLLLMVPSAVCARQDESRIITWQGIERHYAVHRPAQG
                     LPSAAPLVVVLAGLGQDLESLHRWLPIDPVADRHGFIVAYPEAIGGKWSNWRGGGILV
                     PAHATEELDDVGFISAVVETLVKEGTADPKRVYVTGISRGALMSWTLACERADLFSAA
                     APLSSAMTAWQASNCHPSRPLPIVAVDGTEDPVQFYDGFIYPPPVPRLISVPETMAFW
                     WRLSGCTGEKINRLPHGDADDRTRIERYEWTGCAGGGPIMLYRVIGGGHQPPSLATDA
                     SARPEHFGRRSRDMETAEEIWNAFMAVMNTQSP"
     gene            complement(5492..6724)
                     /locus_tag="VGC82_07690"
     CDS             complement(5492..6724)
                     /locus_tag="VGC82_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0423188.1"
                     /translation="MDFNDTPEEAAFRTEARAFLAANAELKAAAKPVLRQSGIDADAV
                     QRCKAWQARKAEAGFAGLTWPKRFGGREASPILQVIYNQEEDAFVVPRGLFEIGLGMC
                     IPTMMTYAKPEQLDRYVRPALHGEEVWCQLFSEPAGGSDLAALRTRAERDGEDWIING
                     QKIWTSGAHLSDFGIIVVRTDPNVPKHEGLTFFFLDMRSPGIEIRPIHQMSGASHFNE
                     VFFTDVRVPDAQRLGAVGQGWKVSLTTLMNERLAVGDVQRPEVDDLLELSRKVRIDGK
                     PAIQNGAVREKIAEWHARSQGLKFTRFRTMTALSKGETPGPENSIHKLVNASKLQDIA
                     SYGLDLMGAGGLVIDDDLAEAYAMFQAALLSSPSSRIAGGSDEILRNIIAERVLGMPP
                     DIRVDKNKPFNQVATGKR"
     gene            complement(6738..>7654)
                     /locus_tag="VGC82_07695"
     CDS             complement(6738..>7654)
                     /locus_tag="VGC82_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0423189.1"
                     /translation="AGLGYEGMCVLAEEIGRAVAPVPFGSTAYLAAEAILTAGSDAQR
                     REYLPKLADGSLIGCLALSEGNGNPEPSGVHARVVGGRLTGAKWPVADGGIADIAVVV
                     ACDEHRQPGLFIAELADVRRRDLRTLDPTRNHARLDFDAKAVAKLGSGGWGAVQRVLD
                     RAAILFAFEQVGGADACLQMAKAYAQERFAFGRPIGSFQAIKHKLADMYVALELARSN
                     AYYGAWALGADATDLALAAATARVSATEAFHLASKENIQTHGGIGFTWDVDCHLFYRR
                     SKQLGLTIGSAPFWKDRLVDLLETRNAA"
BASE COUNT         1391 a         2493 c         2409 g         1361 t
ORIGIN      
        1 gcatacctac gaagcggtcg ccgaggattt ccgcacctgg cggccgaaac tgtccgaccg
       61 tgcggtcgtg ctgtttcacg acaccaatgt gcggcgggat gatttcggcg tgtggcgcct
      121 gtggcgcgaa ctcagcgccg aataccccag cttcgagttc ttacatggcc acggcctcgg
      181 tgtcatcggc gtcggagaaa acctgccggc ggcaatccgg gccctgttcg attgcgatga
      241 gcatgacgat cgccgcacag cggtccggcg gcttttcgcg gctcgtgggc tggctgtcca
      301 gcggacatac gaccttgggt ccacaagcgc gcacgcggcc gcactctcgg gcgacgtgat
      361 gtcgctcaac aacgagacgg cggcgctgcg ggagcaggtt gccacattgc gcgaagagat
      421 cgaaacggtc cgcgccgctg caaccactta tcaggcggca gcggaggcgc ttcaggcgga
      481 cgtggtgtcg ctgcaagccg atgcagcgag ccgcacagct gcgatcgaca gcctccgcga
      541 tgaactggcg gccaaatcgg cgaaggccgc aattctgcag gggcaaatcg acgtcagggc
      601 tgccgaggtc gcggaactga ccgcgcgcct ggtcaatgag gtcgccgagg cgcggcagga
      661 agcggctttg tcgaaacggg catccgaggc ggcggctgac gacgcaatgg cccaggcgat
      721 ggcccggctt gcggcggcgg aggccagatg tgccgcgctt ttggcctcga catgctggcg
      781 aatgaccgcc ccggtacgag cggtgaaaac cgctattttg gagcttcgag cacaggatcc
      841 ggccaaacgg aaccggatga cgcggtggac gagccgcctg acccgccggc ttcctgaccg
      901 cgatgcgccg gatgcggttt tgggcgcgcc cgtggcagcg gcttcgcgcg accggatcat
      961 gccgcctgca tggcatgtcg ggttgctacg gccgcgcgtt ctgatcgtgg cggagctgtc
     1021 gctgccgcaa tgcaagaaat accgcgtcga tcagaagcat gacatgatcg aagcattggg
     1081 ctgggactgt acggtcgtgg cctggcaaca aaccgaccgg gtccggacgc tgattcagac
     1141 gcatagcgtg gtgatcttct accgggttcc ggcatttccg gaggtcctgc cgatcatcca
     1201 ggaggcggac tgcctgggat tgccgcatta cttcgaactg gacgacctga tcttcgatct
     1261 cgacgattat gccgccaatt caaatcttat cgggctcgat cccgccacaa cccggggatt
     1321 gttcgacggt gccaggctgt accgccggat gttggaacta tgccgcaatg tgatcgccag
     1381 cacgccttac cttcaggagc gtatgcacgc ggtcggcggc ggttcgtccg cggtgatcga
     1441 aaatgccctg gatgccgaga cgctgcacgc ggcaaccacg gcacgcccgc gccggccgga
     1501 tggtaagatc cgaatcatct atggatcggg aacgcgaacc cacgatgctg atttcgccat
     1561 gatcgcggac gccctgaagc gcgtcatcac cgagaacggc gacgtcgaga tcgtgctggt
     1621 cggcgacctg aatcccccac cggcattgct cgaattcaac gatcggctgc ggcgcctgcc
     1681 ggcgtgcccg tatcccgagt atctggcctt ggtggcggac agcgacatcg cgatcgcgcc
     1741 gctgatcgcc ggcaacttca acgacgccaa gagcaacatc aagtttcttg aggccgcgtc
     1801 cgtgggcgtg ccctccgtct gttcgccccg ttcggcgttc gcccaggcga tccgccatgg
     1861 cgagaccggc ttcctggcgg cgaccgggga cgactggtac gccgcgctga cctcgctgat
     1921 caccgacgac gcgttgcggc accgcgtggg acaggcggcg cgacaatcag tcctggaatg
     1981 ctatagcccc gaggcgatcg gccgccggca agtcaagccc ctgctgtcga aacatcaacc
     2041 ggccggcgac tcccgcctgc atatccttca ggtcaacatc ctttgcagtc cgcaaacgtt
     2101 cggtggcgcc accgtcgtga tggaggagat gacccagcac ctgaacaacc gggcggatac
     2161 gcgggtcagc atcgtgacca gcgctccggc tgggtggatc aagccttacg ccctggagcg
     2221 cggcgaggtt ctcggcgtcc cggtattccg gattggcatt caggacaacg ccggatggat
     2281 gcaggcgttt tgggacgaag aagcgggcga ggcattccgc gaggcgctgc gggcgttgcg
     2341 gcccgacgtg gtgcattttc acgctatcca gggtctcggg gctgcttgcc tgacggcctg
     2401 ccgcgacgag ggaatcccat tcgtcgtcac gctgcacgat gcctggtggg tgtgcgaacg
     2461 ccagttcctg gtaagcggcg aaggcaagta ttgcggccag aagcggatcg acgcgaacgt
     2521 ttgcgcgaca tgcgtgccgg atatcggggt cacggtgctg cgcaatctgc ggcttcgcca
     2581 ggcgctggag agcgctgacc tgcttctgac cccctcgcag cattggcgcc gcttttacgt
     2641 tgataatgga tttccgccgg atcgcgtcgt cgtcaacgaa aacggcatcc cgctgccggc
     2701 cgacccacaa cccaatgagg gaccggcgcg gctacggttc ggctttgtcg ccggggcgga
     2761 gtcggtgaag gggtttcccc tgctgaagcg cacgttcgaa gccttggacc gggacgactg
     2821 ggagcttgtg cttgtcaacc atggcctctc gatggggctg gattcgtatg ccggcgtcga
     2881 gtggaacgcc cgcgggtcgg ttcgcaccgt tccgccgttc aacaccgagg aacgcgacag
     2941 cttctacgcc tcgatcgacg tcctgctgtt cccgtcccag tggccggaat cctttggcct
     3001 gaccgtacgg gaagcgttgg tgcggaacaa atgggtcatc gccaccggcc agggtggcgc
     3061 cgccgaggca atcaaaccgg gcgtcaacgg cacactgatc ccgattggcg acgacatcgc
     3121 gccattccgt gaggcgatcg aggatctgct cgaccggaag ccggatcttg cgggctggat
     3181 caacccattc gccggccgta cgcgaggctt cgccgcccag gccgccgagc tacgcggaat
     3241 tctgggcgct gtcgcctcct ccggccattc gcagccggcc gacgtggttg cagccgttgc
     3301 atgattagtg gtggatgctt aattgatcgt cattcgaaag accggcgtta ctgagctcgc
     3361 tatcgtggaa gagaactaat ggaagatcgt cctgcggaac tgcttgaagg catcgaccgc
     3421 cggagcaaaa tactggaact cggcccaagt gtatccccca tggcgccacg ctcggccggc
     3481 tgggacgtga ccgtggtgga tcacgcgacg caagaggagt tggtcgaaaa atataaggtc
     3541 gatcccaatg tcaccgtcgc gaacatcgag aaggtcgatt acgtctggag caacggtcca
     3601 ctgcatgaag cggtgccacc cgaactgcac ggcacgttcg acgtcctgat cgccagccac
     3661 gtcatcgaac atctccctaa tccgatcggg ttcctgcaat ccgcggcgcg cctgcttcgt
     3721 ccgggtaccg gtgtcgtagc gctggcggtg ccggacaagc gctggtgttt cgactacttc
     3781 aagcagatca gcagtacagg cgatttcttc gccgcgcatc gtgtgggcgc gcagcggcac
     3841 cggctggcaa cgcgcttcga ccattcagcc tacattgcct tcgacaacgg ccgcggcggc
     3901 tgggggcgag agccgctgac cggcctcaag ctggtcgact cgctggagca cgcctacagc
     3961 gagttccaat cctggaccga cgatccatcc gcaccctacg tggactgcca tgcctggcac
     4021 ttcacgccgg ctagcttcga actgctgatt ttggaagccg gcgcggtcgg attgctggac
     4081 tggcggttgg cgtggctgca tccgcgcgcc ggagtagaat tcttcgcccg cctggttccg
     4141 gggcatgaac agttcgccac cgtggccgat cgcgaaagtc gcaggctgga actgctcaaa
     4201 cagattctgc tcgatgttcg cgaacagacc gactggctgg tcgagccgcg acccggcatc
     4261 agccaagccg tcgcgcccga catcagccaa accgtcgcgc ccgacatcgg ttttgatgcc
     4321 aatgcgcggg agcaacttcg gcagatcgct gaaaccgcgg ccgaagtacg cgctgcactt
     4381 cgtcccggcc gagcggtctg gcaggggatg ctgccgttgc ggcgtcagat cgctcggctt
     4441 cgtggcaaaa gctaacgagg cgagccttgt ccactgccta cccctgggat cggggcagcc
     4501 tgaaataatg gcagcctggg gtcatggtga ttgcgtattc ataacggcca tgaaggcatt
     4561 ccaaatctcc tccgccgtct ccatgtccct gctgcgccga ccgaagtgct caggccgggc
     4621 ggaagcatcg gtcgccaacg acggcggctg atgcccgccg ccgatcactc gatacagcat
     4681 aatggggccg ccacccgcgc atccggtcca ttcatatcgt tcgatgcgtg tacgatcatc
     4741 ggcgtcacca tgtggcagcc ggtttatctt ctccccggtg cagccgctta gccgccacca
     4801 gaaagccatc gtctccggga ccgagatgag gcgcggcacc ggaggcggat agatgaagcc
     4861 gtcatagaac tgcacagggt cttccgtgcc atcgacagca acgataggca gcggccgtga
     4921 cggatggcag ttggaagcct gccatgcggt catcgccgaa gacaggggtg cggcagcgct
     4981 gaaaagatcc gcgcgctcgc acgccagggt ccaactcatg agtgcgcctc gcgaaatgcc
     5041 ggttacgtac acacgctttg gatcggccgt gccttcctta acgagcgtct cgacaacagc
     5101 agagataaag ccaacgtcat caagctcctc agtggcgtga gccggaacca ggatgccgcc
     5161 accgcgccaa ttgctccact tcccgccgat cgcttcggga taggcgacga tgaagccatg
     5221 ccggtctgca acgggatcga taggcaacca gcgatgcaga ctttcaaggt cctgccccag
     5281 cccggccagg acaacgacca atggcgcggc ggaaggcaga ccctgtgccg ggcggtgcac
     5341 cgcatagtgc cgctcaatgc cttgccacgt aatgatgcgg gactcgtcct gccgggcaca
     5401 aacggcactg ggcaccatca gcagcaggac gacgaagatg acgccgcgca tctacagtca
     5461 ggccgcgtat gtacgatcag attgaatgct tctaccgctt gcccgtcgca acctggttga
     5521 acggcttgtt cttgtccaca cgaatgtctg gcggcatccc aagcacccgt tcggcaatga
     5581 tgttccgcag gatctcgtcc gacccgccgg cgatgcggga acttggcgac gacagcaacg
     5641 cggcctggaa catcgcatag gcctccgcaa ggtcgtcgtc gatcaccagc ccgccggccc
     5701 ccatcaggtc caacccgtag ctggcgatat cctgcagctt cgaagcgttc accagcttgt
     5761 ggatggagtt ctccggcccg ggagtctcgc ccttcgacag cgccgtcatg gtgcggaacc
     5821 gggtgaactt caaaccctgg gaccgcgcgt gccattcggc gattttctcc cgcaccgcgc
     5881 cgttctggat cgccggcttg ccgtcgatcc gcaccttgcg gctcagttcc aacaaatcgt
     5941 ccacctccgg ccgctgcacg tcgccgactg cgaggcgttc gttcatcagc gtggtcagcg
     6001 acaccttcca cccctgcccc acggcgccga gacgctgcgc atcgggcacc cggacatcgg
     6061 tgaagaacac ctcgttgaaa tggctcgccc cggacatctg gtggatcggc cggatttcga
     6121 tgccgggcga cctcatgtcc aggaagaaaa aggtcagacc ctcgtgtttc ggcacattcg
     6181 gatcggtgcg cacgacgatg atgccgaaat ccgacagatg cgcccccgag gtccatatct
     6241 tctgcccgtt gatgatccag tcctcgccat cccgttccgc ccgcgtgcgc agcgccgcga
     6301 gatccgaccc gccggccggc tcgctgaaca actggcacca cacttcctcg ccgtgcaggg
     6361 cgggccgcac atagcggtcg agctgttccg gcttcgcata ggtcatcatg gtcgggatgc
     6421 acatgcccaa tccaatttcg aaaaggccgc gcggcacgac gaaggcgtct tcttcctggt
     6481 tatagatcac ctgcaggatc ggcgatgcct cccgcccgcc gaagcgcttc ggccaggtca
     6541 ggccggcgaa tcccgcttcg gccttcctcg cctgccaggc cttgcagcgc tgaaccgcgt
     6601 cggcatcgat accgctttgc cgcaacacgg gtttcgccgc ggctttcaac tcggcattgg
     6661 cggccaaaaa ggcgcgtgcc tcggtacgaa acgcggcttc ttccggtgtg tcgttgaagt
     6721 ccatgatggc gttcccttca ggctgcgttg cgggtttcca gcagatccac cagccggtct
     6781 ttccagaacg gcgccgaccc gatcgtcaag ccaagctgct tggatcggcg atagaacaaa
     6841 tggcagtcaa cgtcccaggt gaagccgatg ccgccatgcg tctggatgtt ttctttcgag
     6901 gccaggtgga acgcctcggt cgccgatacc cgcgcggtcg ccgcggccag cgccaaatcg
     6961 gtcgcatcgg cacccagcgc ccaggcaccg taataggcgt tggagcgggc cagctccagc
     7021 gcgacgtaca tgtccgcgag tttatgcttg atcgcctgga acgatccgat cggccggccg
     7081 aacgcaaacc gttcctgcgc atacgccttc gccatttgca gacaggcgtc cgcgccgccc
     7141 acctgctcga acgcaaacaa gatcgccgct cggtcgagca cgcgctgaac cgcgccccag
     7201 ccgccggagc caagctttgc caccgccttc gcatcgaaat ccaaccgagc gtggttgcgg
     7261 gtcgggtcga gcgtacgcag gtcgcgccgg cgcacgtcgg ccagttcggc gatgaacagg
     7321 ccgggctggc ggtgttcgtc gcaggcgacg acgacggcga tatcggcgat cccgccgtcc
     7381 gccaccggcc atttcgcacc cgtcagccgg ccgccgacga cgcgggcatg aacaccggag
     7441 ggttccggat tgccgttgcc ctcggacaac gccaggcagc cgatcaacga gccgtcggcc
     7501 agcttcggca ggtattcccg ccgctgcgca tccgatccgg cggtcaggat cgcctccgcc
     7561 gccagatagg cggtcgatcc gaacggcacc ggcgcgaccg cgcggccgat ttcctccgcc
     7621 agcacgcaca tgccttcgta gccgagaccg gcgc
//
LOCUS       DASWQN010000469         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1892678, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000469 DASWQN010000000
VERSION     DASWQN010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892678"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VGC82_07700"
     CDS             complement(<1..448)
                     /locus_tag="VGC82_07700"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY0423190.1"
                     /translation="MNSYQLTAFGRPLELRCEEEPEPKGAEVLVRIERSGVCHSDLHI
                     WSGFFDLGGGKRFQMTDRMTLPFTLGHEIVGEVIATGPEAEGVAVGQKGIVHPWIGCG
                     TCHACGAGQELRCTTPRTIGTRRNGGYSDRVLVPHGRYIVPYGNLDP"
     gene            complement(546..1355)
                     /locus_tag="VGC82_07705"
     CDS             complement(546..1355)
                     /locus_tag="VGC82_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY0423191.1"
                     /translation="MQLWHRERSGAMHMRSSIAFAGMLAASLLHAAPGHAQQMRTWVS
                     GTGDDANPCSRTAMCKTFTGAISKTASGGEISVYDPGGFGSVTINKSISIVDVGAKAS
                     MLVAGTTGVTIKAGPTDVVLLDGLVLEGGGTATTGISIVSAGSVHIRDCAIRGFQAEP
                     GLAIDVAPTAATQVFVSNCAIGKNSAGIRVKPADSGSARVFLERVDLENNAGSAIRAE
                     GPNAVIRLNRTAITNNGAGLDLAGGSIITFGTNAISGNTVDGQPSHIERLQ"
BASE COUNT          267 a          565 c          538 g          295 t
ORIGIN      
        1 cggggtcgag gttgccatag ggcacgatgt agcggccgtg cgggacaaga acgcggtcgc
       61 tgtagccgcc gtttcgccgg gtgccgatgg tgcgcggggt tgtgcagcgc agttcctgcc
      121 cggccccgca ggcatggcag gtgccgcagc cgatccaggg atggacgatg cccttctgcc
      181 cgaccgcgac gccttcggcc tccggcccgg tcgcgatcac ctcgccgaca atctcgtggc
      241 cgagcgtgaa gggcagcgtc atccggtccg tcatctggaa gcgcttgccg ccgccgagat
      301 cgaagaagcc ggaccagata tgcaggtccg aatggcagac cccggagcgc tcgatccgga
      361 ccagcacctc cgcgcccttc ggctcaggct cctcctcgca gcgcagttcg agcgggcggc
      421 cgaaggctgt gagttggtag ctgttcatgt cccctcccgg ccggcacgtt ggccggacga
      481 gcggatcgtc agcgcgccca tcacaaagtc aagcttgcga gctgcttagg cccgagcgct
      541 tggccttact gtaaccgctc tatgtgggac ggctggccgt ccaccgtgtt cccactgatg
      601 gcattcgtgc cgaaggtgat gatgctgccg cccgcgagat ccagcccggc gccgttattg
      661 gtgatggcgg tccgattcaa ccggatgacg gcattcgggc cctcggcacg gatcgcgctg
      721 ccggcgttat tctcgagatc cacgcgctcc aggaagacgc gggcgcttcc gctatcggcc
      781 ggcttgaccc ggatgcccgc gctgttcttg ccgatggcgc agttcgatac gaagacctgg
      841 gttgcagccg tcggcgcaac atcgatcgca agcccaggtt cggcctggaa ccctcggatg
      901 gcgcaatcac ggatatgtac cgagcccgcc gaaacgatgc tgatcccggt tgtggcggtg
      961 ccgcctccct cgagcaccaa gccgtcgagc agcacgacgt cagtcgggcc cgccttgatg
     1021 gtcacgcccg tcgtgccggc caccagcatg ctggccttgg caccgacgtc gacgatgctg
     1081 atggacttgt tgatcgtgac cgacccgaag ccgcccgggt cgtaaacgga gatctcgccg
     1141 cccgacgcgg tctttgagat ggcccccgtg aatgtcttgc acatcgccgt gcgcgagcat
     1201 ggattggcgt catcacccgt gccggacacc caggtgcgca tctgctgcgc gtggcccggg
     1261 gcggcatgga gcagggaggc ggccagcata ccggcaaacg caatcgagct tcgcatgtgc
     1321 atggccccgc tccgttctcg atgccacagc tgcatccagt gatctgctcg cgcgtgccac
     1381 cgcagcccgg cagcattggc tgacaagcat gtcagtctat tggaaccgtt cgcggattgg
     1441 ccatcggcgc aatcgcaccg cacctggcaa gatgagctgt gcgggcgtcg gcctcggatt
     1501 tcagccaggc tctttgcgcc tgcaaacacc atccgtaatg ggcgttggaa ttcgcctgcc
     1561 agcgcccgtc cgttcggaca gcgcacctcc cgccgcgaga tcgagcaagc tcgtaacgct
     1621 ccacggcctt ccgggcatag tcccggcatt caccggcgct cgccg
//
LOCUS       DASWQN010000470         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_440835, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000470 DASWQN010000000
VERSION     DASWQN010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440835"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..>1816)
                     /locus_tag="VGC82_07710"
     CDS             complement(295..>1816)
                     /locus_tag="VGC82_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEY0423192.1"
                     /translation="PATGLVALPGEVAFRLYDTYGFPLDLTQDALREQGRGVDLEGFE
                     AAMQEQRTRARAAWAGSGDAATDRIWFEIKERAGASEFLGYTTESAEGEILAMVVDGV
                     SNDAAPAGTNVAVVLNQTPFYGESGGQVGDTGTITGPDGLVITVTDTQKKLGDLFVHL
                     GRVEAGVAKTGVPVVATVDHERRGAIRAHHSATHLLHEALRRKLGTHVQQKGSLNAPD
                     RLRFDVSQPRPITGDELHWVEREVNARIRENSEVTTRLMTPEAAVELGAMALFGEKYG
                     DEVRVVAMGEPDGNKPAWSIELCGGTHVRRTGDIGYFRIVGEAAVAAGVRRIEAVTGA
                     SAEAVIDETQARLNDAAGALRAAPAEVPDRIAALLEDRRKLERQVAELQKRLATGGGA
                     AAAEDIGGIMLSARNLGEVPAKDLKGLADAIGKQQGSGVVALVSTAEGKASIVVAVSS
                     DLTSRISAVDLVRAASAAVGGKGGGGRPDLAQAGGPDGTKADEALAAVRLALSGTL"
BASE COUNT          289 a          657 c          530 g          340 t
ORIGIN      
        1 cggccccgtg gcgatgtagt ttctaatttc gtgacttcgg cagtctttag ggtgacaaaa
       61 taaccgctcg acattaccgt ctggcgattc gcactggaag tttggtttca ctccggttgc
      121 cgtcggactg cacccgcccc gaaaatcgcg gcgtccatgc tcgagtttct gagccatagc
      181 taagacggcg cccaacttca ccgtcaaaat ccatcggctt gcgttcgccg ggagctgttc
      241 gtgcggtata tcgatctttt tactgtagca gatatcactt gaggggccgg aagtttacag
      301 cgttcccgaa agcgccagcc gcacggccgc cagcgcttcg tccgccttcg tcccatcagg
      361 cccccccgcc tgagccagat ccggccggcc accgccgccc ttgccgccga ccgccgccga
      421 agccgcacgc accagatcga cggcgctgat ccggctcgtc aggtcggacg aaaccgcgac
      481 gacgatgctg gccttgccct cggcggtcga tacaagcgcc accacgcccg aaccctgctg
      541 cttgccgatc gcgtcggcca ggcctttcag atccttggcc ggcacctcgc caaggttgcg
      601 cgcggacaac atgatcccgc caatgtcttc ggccgccgcc gccccgcctc cggtcgcgag
      661 ccgtttctgc aactcggcca cctggcgttc cagcttgcgc ctgtcctcca ggagtgcggc
      721 gatgcggtcc ggcacctcgg ccggagccgc ccgcagcgcg cctgcggcat cgttaagccg
      781 ggcctgtgtt tcatcgatca ccgcttccgc cgacgctccg gtcacagcct cgattcgacg
      841 cacgccggcg gctactgccg cctcgccgac gatgcgaaaa tatccgatgt cgccggtgcg
      901 ccgcacatgc gtgccaccgc aaagctcgat cgaccatgcc ggcttgttgc cgtccggctc
      961 gcccatcgcg acgacgcgaa cctcgtcgcc gtatttttcc ccgaacagcg ccatcgcacc
     1021 aagttcgacc gcagcctccg gcgtcatcag ccgcgtcgtg acctcggagt tctcgcggat
     1081 gcgcgcattc acctcgcgtt cgacccaatg cagttcgtcg ccggtgatcg ggcgcggctg
     1141 gctgacatcg aagcgcagcc ggtcgggcgc attcaggctg cctttttgct gcacatgggt
     1201 gccgagtttg cgacgcaacg cttcatgcag cagatgggtg gcggaatgat gcgcccggat
     1261 cgccccgcga cgctcatgat ccaccgttgc caccaccggc acaccggtct tcgcgacacc
     1321 ggcttcgacc cggccgagat ggacgaacag gtcaccgagc ttcttttggg tgtcggtcac
     1381 ggtgatgacc aggccgtccg gcccggtgat cgtaccagtg tcgccaacct gaccgccgct
     1441 ttcgccatag aacggggtct ggttcagcac caccgcgacg ttggttccag ccggagcggc
     1501 atcgttggat acaccgtcca cgaccatggc caggatttcg ccctcggcgc tttcggtggt
     1561 gtagccgagg aactccgacg cccctgcccg ctccttgatc tcgaaccata tccgatcggt
     1621 ggccgcatcg cccgaacccg cccaggcagc gcgcgctcgg gtgcgctgtt cctgcatcgc
     1681 ggcttcgaaa ccttccagat cgacgccgcg cccttgctcc cgcagcgcgt cctgcgtcag
     1741 gtcgagtggg aacccatagg tgtcgtaaag cctgaacgcg acctcaccag gcagggcaac
     1801 caggcccgtt gcgggc
//
LOCUS       DASWQN010000471         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1760649, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000471 DASWQN010000000
VERSION     DASWQN010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760649"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1372
                     /locus_tag="VGC82_07715"
     CDS             107..1372
                     /locus_tag="VGC82_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEY0423193.1"
                     /translation="MSIHVPAKHPTWLPTLLGLFIAVGPAATDMYLPAFPAVEASFGS
                     APGTAQLTLATWFAGLAIGQITQGPLSDRFGRRFPLMLGFAVFTLSTIGCALAPSLTW
                     LAIFRTTAAFGASAGMVISRAVVRDLAEGQAAAIMMSRLVLVMGVAPILAPSVGGAIL
                     AFAHWRVIFWVLTIYGAACVLTAWRTLPETLAGEHRIRLSLAGQASRYLTILREKTFL
                     THAIMGGCSTFCMFAYLAGCSPVFELGFGLSPSQFALIFGLCAVSLVGCSQLNARLLP
                     RFGLSRMLTAITWISFCGTVVLLVLAFSGVHILWAIVAPLVLIVGAQGFTGANATAGA
                     LVRHASHAGSASALMGTFQFSLGASSGVLAGLLTDGTPKGMAALMFCGMLGAVIADRF
                     RRRSEIAQPKVAGSKAAGLDIARSQVAPP"
     gene            1369..>1561
                     /locus_tag="VGC82_07720"
     CDS             1369..>1561
                     /locus_tag="VGC82_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY0423194.1"
                     /translation="MIVGIPACAKTLSDMQFHQTPARYAAVVLGGCGALPVLIPPLGE
                     QMLGLLDDLAGLLVPGSASN"
BASE COUNT          210 a          572 c          446 g          333 t
ORIGIN      
        1 gacgacacgg acggtgccaa gacaaccctt gaccccatcc tgctttcccc gcaacaaccg
       61 cccggggacc gacgcccatc caccttaacc gcaagtgccc tcccggatga gcatccacgt
      121 tcccgccaag caccctacct ggctacccac cctcctcggc cttttcatcg ctgtcggccc
      181 cgctgcgacc gacatgtacc tgcccgcgtt tccggccgtc gaagccagtt tcggttccgc
      241 acccggcact gcgcagctta cgcttgcgac ctggttcgcc ggcctcgcca tcggtcagat
      301 cacccaaggc ccgctgtccg accgcttcgg ccgccgcttc ccgttgatgc tgggcttcgc
      361 ggttttcacc ctcagcacca tcggctgcgc tcttgcgccg tcgctgacct ggctcgcgat
      421 ctttcgcact accgccgcat tcggcgcctc cgccggcatg gtgatttccc gagccgtcgt
      481 gcgcgatctg gccgagggcc aggccgcggc catcatgatg tcccgcctcg tccttgtcat
      541 gggcgtcgcc ccgatcctgg cgccgtccgt tggtggcgcc atcctcgcct tcgcccactg
      601 gcgtgtcatt ttctgggtcc tgacgatcta tggcgccgcc tgcgtcctga ccgcctggcg
      661 aacgcttccc gaaacattgg ccggagaaca tcggattcgc ctgtccctcg ccggccaggc
      721 ttcgcgctac ctgacgatcc tgcgcgagaa aaccttcctc acccacgcca tcatgggcgg
      781 ttgcagcacg ttctgcatgt tcgcctacct ggccggttgc tctccagtgt tcgagcttgg
      841 ttttggcctc agcccgtcgc agttcgcact gattttcgga ctttgcgcgg tcagccttgt
      901 tggttgctcg cagctcaacg cccgcctgtt gccccgattc ggcctgagcc ggatgctgac
      961 cgcaatcacc tggatttcct tttgtggcac tgtcgtgctg ctggtgctgg ccttcagcgg
     1021 cgtccacatc ctgtgggcga tcgtggctcc cttggtgctc atcgtcggcg cccaaggctt
     1081 caccggcgcc aacgcgacag ccggagcgtt ggtgcgccac gccagtcatg ccggcagtgc
     1141 ctcggcgctc atgggcacgt tccagttcag cctgggtgcg agttccggtg tgctggccgg
     1201 tttactgacc gacggtaccc ccaagggcat ggcggcgctg atgttttgtg gcatgctggg
     1261 cgcggtgatc gccgaccggt tccgtcgcag gtcagaaatc gcccaaccaa aagtcgccgg
     1321 ttcaaaagcc gccggtttgg acatcgcccg ttcacaggtc gccccgccat gatcgttggc
     1381 attcccgcct gcgccaaaac gctttcggac atgcagttcc accagacgcc cgcccgatac
     1441 gccgcggttg ttctgggcgg ttgcggcgcc ttgccggtcc tgatcccccc attgggcgaa
     1501 caaatgctcg gcctgctcga cgatcttgca ggcttgctgg tcccgggcag cgcgagcaac
     1561 g
//
LOCUS       DASWQN010000472         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_177285, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000472 DASWQN010000000
VERSION     DASWQN010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177285"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VGC82_07725"
     CDS             <1..462
                     /locus_tag="VGC82_07725"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEY0423195.1"
                     /translation="VGIGTLYLDRIALAVERRFYPWLPRPESAPVLEQAWDGIIVALN
                     VLALNVAALVLALVLPGIGFILGWMIAAYAIGRSLFVAVAMRRMPRAMAESLYRRSRG
                     LVLTQGAMLALSAYVPVANLLIPVLGTAAMVHILDMALSAAHGSQFQTNGQ"
     gene            828..1316
                     /locus_tag="VGC82_07730"
     CDS             828..1316
                     /locus_tag="VGC82_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY0423196.1"
                     /translation="MSRPPFSSAPAAPSGPATLARPGVTVIRPAARDPSERRTLVVGR
                     GISLQGVVQDAERLVVEGTVEATMIHATELAIAPGGVFKGEVEVEDAEIAGTIDGTLT
                     ARGSLIVRSSGRVLGTARCRRLQVEDGGQITGRIEMITDQSHGDLPQSDLASRQNVEP
                     AI"
BASE COUNT          263 a          488 c          525 g          287 t
ORIGIN      
        1 gtcgggatcg gcacgttgta tctcgatcgc atcgcactgg cggtggagcg aaggttttat
       61 ccgtggcttc ctcggccgga gagtgcgccg gtgttggaac aggcatggga cggcatcatc
      121 gtggcactga acgtattggc gttgaatgtg gctgccttgg tcctggcgct tgtcctgccg
      181 ggaatcggct tcattctggg ctggatgatc gccgcctatg cgatcggccg cagcctgttc
      241 gttgccgtgg cgatgagacg gatgccgcgg gcgatggccg agtcgctgta ccgccgaagt
      301 cggggccttg ttctgacgca gggcgcgatg ctggcattgt cggcctatgt tccggttgcg
      361 aacctgctga taccggtgct tggcaccgct gccatggtgc atatcctcga tatggcgtta
      421 tcggcggctc acggatcgca gtttcagacg aacgggcaat aagtccgcaa ccggatgaat
      481 gtggatagtt gtcacaagag ggtgttcggg aggctcgctg cgtgctaggc ggaggcacat
      541 tttcagtctg gcggagtgcc ttgttcaagt ggcttaaacc cgacgagccg cccccggcac
      601 cgaccccgct gcctgcggga ctggggctgg tgccgcccga ttccacgcca ggcaagtccg
      661 ggccgggcga gtccgggcca agcaagtccg ggccgggcga gtccgggcca ggcgggtccg
      721 gttcggatat ccgcacggtc ccgccgcgtg ccgcaccatc atcatcgttc gacaacgggc
      781 ttggcgttcc accgttccgt cccgctccgc ttaaggatac gcataacatg tcccgaccgc
      841 cgttttcatc tgcccccgcc gcaccctccg gtcccgccac cctcgctcgg ccgggggtaa
      901 ccgttattcg cccggccgcc cgcgacccat ccgaacgccg cacgctggtg gtcgggcgcg
      961 gcatcagtct gcaaggcgtg gtgcaggacg ccgaacgcct ggtcgtggaa gggaccgtcg
     1021 aggcgacgat gatccatgcg accgaacttg cgatcgcgcc gggcggcgtt ttcaagggtg
     1081 aggtcgaggt ggaggatgcc gaaatcgccg gcacgatcga cggcacgctg accgcccgag
     1141 gcagcctgat cgttcgcagc agcggccgag tgttgggaac cgcgcgctgc cggcggctgc
     1201 aggtggagga tggcggccag atcaccggcc ggatcgagat gatcacggat cagtcgcacg
     1261 gcgacctgcc gcagagcgac ctggcctcgc ggcagaacgt cgagccggcg atctaagcgc
     1321 ggctatcggg ggggggcgga ccgtcccgtc aaacatgggg cccctgtgcc aaccaaccac
     1381 ggccatgaca ttagctagta cccacaatca aagaagcgcg aaacaaacgt ccgacggcca
     1441 gtcctccaag aaggccagta tgatgccccc cgggcgtatc gcgcttcaat gatagtgggt
     1501 actagccatt cttttcgacc tgccgcatcc atttgcctag catgggcgcg tccgatgaca
     1561 tgc
//
LOCUS       DASWQN010000473         4784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_45882, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000473 DASWQN010000000
VERSION     DASWQN010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45882"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1626)
                     /locus_tag="VGC82_07735"
     CDS             complement(64..1626)
                     /locus_tag="VGC82_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HEY0423197.1"
                     /translation="MLIGQATVHGSVASFYGIDPSTGREIEPLFGSGSIADVERACVL
                     AQQAFDGYRDTALEQRALFLEQIAQNIVDLGPALIDRAMQESGLPQARLEGERGRTVG
                     QLRLFAKVVRDGRWIEATLDSALPQRTPPRPDLRLRKIGLGPVAVFGASNFPLAFSVA
                     GGDTASALAAGCPVVVKAHSAHPGTSELVGKAVQAAVASCGLHEGVFSLLFGDGRTVG
                     QALVSHPAIKAVGFTGSRQGGMALVRTAAQRDEPIPVYAEMSSINPMFLLGGAMAQRA
                     REIGTGFIDSLTLGAGQFCTNPGMVIAVEGDALNTFCDAAATALQAKVASTMLTPGIH
                     AAFTQGVERLAATPGVAALARGQKNETSVHSGQGALFKTDAATFLADPSMEEEIFGAS
                     ALIISCPDASRFRQIAEHLDGQLTATVFMTEKDRDAALELLPTLERKVGRILVNSYPT
                     GVEVSHAMVHGGPFPATSDSRSTSVGATAIERFLRPVSYQDLPAYLLPASLQDENPLG
                     LWRVRDGELTQT"
     gene            complement(1761..2675)
                     /gene="kdgD"
                     /locus_tag="VGC82_07740"
     CDS             complement(1761..2675)
                     /gene="kdgD"
                     /locus_tag="VGC82_07740"
                     /EC_number="4.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047448 - 5-dehydro-4-deoxyglucarate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HEY0423198.1"
                     /translation="MYQPQDLKQILSSGLLSFPVTDFDANGNFRADTYTSRLEWLAPY
                     GATALFVAGGTGEFFSLTPADYSQVVKVAVDTCRDKVPILAGAGGPTRVAIGYAQEAE
                     RLGAHGILLMPHYLTEASQDGLVEHFTEVCNSVKCGVVVYNRQVSRLTAESCLKLADR
                     CPNMVGFKDGIGDIELMVTIRRKLGDRFTYLGGLPTAEVFAPAYKAIGVPVYSSAVFN
                     FIPKTAVDFYEAVKNDDYATTDRLIEDFFLPYLEIRNKKHGYAVSIVKAGATIVGHDA
                     GPVRPPLTDLTGEEREQLKALIDTLGPQ"
     gene            complement(2768..2992)
                     /locus_tag="VGC82_07745"
     CDS             complement(2768..2992)
                     /locus_tag="VGC82_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423199.1"
                     /translation="MQVVPVAGHDSMLMNLSGAHGQKPGLGVELDFEQLEKHHQLYKD
                     TSAGARDDAAAMQFLIPGWKYDNKRPALVR"
     gene            complement(3437..4300)
                     /locus_tag="VGC82_07750"
     CDS             complement(3437..4300)
                     /locus_tag="VGC82_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0423200.1"
                     /translation="MEDKPRSKLLARHRHDCYQIVWVTLGRGIHVIDWEIYPVREGAI
                     FSLAPGRVHSWIFGDSTPADLNSVPSPVSIAGYVLEFDAAFATSWINPEAQQVLLPSL
                     VDIRSLALYIDAEQSKALREIVSNLEWEYKHKELGHAGALQAYFQLFLIRLSRYRSVH
                     HPTHGFNKEEALVQRFRILVDGHFWERLSLQRYAATLGVSKGHLNRMVRKQTGMTASN
                     LVQERIVVEAKRMLTHMSTSILDIANSLGFKDPAYFARLFKKRTGQTPKQFKSAFHDT
                     LKAISADTLKK"
     gene            complement(4424..>4784)
                     /locus_tag="VGC82_07755"
     CDS             complement(4424..>4784)
                     /locus_tag="VGC82_07755"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY0423201.1"
                     /translation="LGNPTGFHLTPGQAHDLDGADVLLKDTPAQVVIADKAYDAHQRV
                     IEPLRKAGKEIVIPQRGANRKPPRECDHHLYKARHLIENFFAKLKQYRCIATRYDKTA
                     RNFLGAIYCVASVIWLD"
BASE COUNT          909 a         1388 c         1495 g          992 t
ORIGIN      
        1 gtggatcgat gcatcggtgc cgatctcggg gacgatttcg gcaaaaatgg tcggcgagcg
       61 gcgtcaggtt tgggtcaatt cgccgtcacg cacgcgccac aagccgagcg ggttctcatc
      121 ttgcagggaa gcgggcagca agtacgcggg caaatcctga taactgaccg gacgcaggaa
      181 acgttcgatg gcggtggcac cgaccgaagt gctgcggctg tccgaggtcg cagggaaggg
      241 gccgccgtgc accatcgcat gcgacacttc gaccccggtc ggataactgt tgaccagaat
      301 tcgtccgacc ttgcgttcca gcgttggcaa cagctccagc gccgcgtccc ggtccttttc
      361 ggtcatgaag acggtggcgg tcagctggcc gtcgaggtgt tccgcgatct gcctgaatcg
      421 gctggcatcg gggcagctga tgatcagcgc gctcgcgccg aaaatctctt cttccatgga
      481 gggatcggcg aggaaggttg cggcatcggt tttgaacaac gcgccttggc cggagtgcac
      541 gctggtttcg tttttctgtc cacgcgccag cgcagcgacg ccgggcgtag cggcgagccg
      601 ctccacgccc tgagtaaagg cggcgtggat tccaggcgtg agcatggtgc tggcaacctt
      661 ggcttgcaag gcggtcgcag cagcgtcgca gaacgtgttc agtgcatcgc cttccaccgc
      721 gataaccatg ccgggattgg tgcaaaactg accggctccc agggtaaggg aatctatgaa
      781 gccggtgccg atttccctgg cacgctgcgc catggcaccg cccagcagga acatcggatt
      841 gatgctgctc atttccgcgt ataccggaat cggctcgtcg cgttgcgccg ccgtgcgcac
      901 cagcgccatg ccgccctggc gcgagccggt aaagcccacc gccttgatgg ccgggtggct
      961 taccagcgcc tgaccgaccg ttctgccatc gccgaacagc agggagaaca cgccttcgtg
     1021 cagaccgcag ctggcgaccg cggcctggac tgccttgccc accagttcgg aggtgccggg
     1081 gtgggcgctg tgcgccttga ccacgaccgg gcagccggca gcgagcgcgg aggcggtatc
     1141 gccgcccgcg accgaaaacg ccagcgggaa attgctcgcg ccgaaaacgg cgaccggtcc
     1201 caggccgatt ttgcgcaggc gcaggtcggg gcggggcggc gtgcgttgcg gcagcgcaga
     1261 atcaagcgtc gcttcgatcc agcggccgtc gcgcaccacc ttggcgaaca ggcgcagctg
     1321 cccgaccgtg cgcccgcgct cgccttcaag gcgggcctgc ggcagccccg attcctgcat
     1381 cgcccggtcg atcagcgcgg ggccgagatc gacgatattc tgcgcgatct gctccagaaa
     1441 cagcgcgcgc tgctccagcg ccgtgtcccg gtaaccgtcg aatgcctgtt gcgcgagcac
     1501 gcatgcgcgc tcgacgtcgg caatgctgcc ggagccaaac agcggttcga tctcgcgtcc
     1561 ggtggagggg tcgatgccat aaaatgaagc gacgctgcca tgcacagtcg cttggccgat
     1621 cagcatgtcg ccgttgatgc ttgtgttcaa gatattctcc tgtgaacgga tgcggcgcac
     1681 cgaatcagca gtgccgtgga gcgcccggat cgaatcgtga agtctggcgc gcccgcgcga
     1741 cgcgggcgcg caatgcgcct ttactgcggg ccgagcgtat cgatcagtgc tttcaactgt
     1801 tcgcgctctt caccggtgag atcggtgagc ggcgggcgca ccgggccggc atcgtggccg
     1861 acgatggtcg cgccggcctt gacgatgctg accgcgtagc cgtgcttctt gttgcggatc
     1921 tccagatacg gcaggaagaa gtcctcgatc aggcggtcgg tggtggcgta atcgtcgttc
     1981 ttgactgctt catagaaatc gaccgcggtc ttcggaatga agttgaacac ggccgacgag
     2041 tagaccggca cgccgatcgc cttgtacgcc ggagcgaaca cttcggccgt cggcaagccg
     2101 cccagatagg tgaaacggtc gcccagcttg cgccggatcg tgaccatcag ttcgatatcg
     2161 ccgatgccgt ccttgaaacc gaccatgttg ggacagcggt cggccagctt cagacacgat
     2221 tccgcggtca ggcgcgagac ctggcggttg tagaccacaa cgccgcactt gaccgagttg
     2281 cacacctcgg tgaaatgctc gaccagtccg tcctggctcg cttccgtcag gtaatgcggc
     2341 atcaacagga tgccgtgagc gcccaagcgc tcggcttcct gggcgtagcc gatcgcaacg
     2401 cgggtcgggc cgcctgcgcc cgccaggatg ggtaccttgt cgcggcaggt atcgactgcc
     2461 accttcacca cctggctgta gtcggccggg gtgagcgaga agaattcacc ggtgccgccg
     2521 gccacgaaca aggcggtcgc gccgtatggc gccagccatt cgagacggct ggtgtaggtg
     2581 tccgcgcgaa agtttccgtt ggcgtcgaag tcggtgacgg ggaacgacag caggccggac
     2641 gagaggatct gtttcaggtc ttgtggttgg tacatgatgt gcgagcctta aaagcgagag
     2701 tgtggaaaaa gagggcgaaa gagggcgttt acgggttttg cgtcacttgc tgcggtggcc
     2761 gccgggttca acgaaccagc gccggtcgtt tgttgtcgta cttccatccg ggtatcagga
     2821 actgcatcgc cgccgcatcg tcgcgtgcgc cggcgctcgt gtccttgtac agttgatggt
     2881 gcttctccaa ctgttcgaag tcgagttcga cgcccaggcc gggtttttga ccatgggcgc
     2941 cactcaggtt catgagcatg ctgtcatggc cggccaccgg aaccacttgc atctcggtca
     3001 cccgcgacgc accgctcgtt cgacgaagtc attgaagaca tgtctgttcc tttccaattt
     3061 ggagtttgct acaggacagg cggcctcaag ggtcagcccg cgattcactc cggacgtttt
     3121 gcataacaca gccggagttg atatctcttc gccatcatgc agaggcggga ccgaggacgc
     3181 caaaaccaat gcgccacttt gccgcgatgg caagccaagc ttgttgccag ccttgtaacc
     3241 gattcagctc ctcctccgcc gatatgccga tcgaatggat aagtggggcg attaatggat
     3301 attgccctct tgcgggtacg tcatcgtacg gccgacccgc gtttataaag tgcgagtgta
     3361 ctgatccagc cctggcagcg ttaggacaga tcgtgcattt tcttgcattt gatgactatc
     3421 agcagggaac ccggaatcat tttttaagag tatcggcgct tatggctttt aaagtgtcat
     3481 ggaacgctga cttgaattgc tttggtgtct gtccggtgcg tttcttgaac agccgggcaa
     3541 agtacgccgg gtctttgaag cccaacgaat tcgcgatgtc caaaatgctt gtggacatat
     3601 gagtcagcat gcgcttggct tcgaccacta tgcgctcctg cacgaggtta ctagccgtca
     3661 tcccggtctg cttccgtacc attcggttta gatgtccctt cgacaccccg agtgttgcag
     3721 catagcgctg tagcgataac ctttcccaga agtgtccatc gaccaagatg cggaagcgtt
     3781 gcaccagcgc ttcctccttg ttgaagccgt gtgtaggatg gtggactgat cggtagcggc
     3841 tcagccggat cagaaacagc tgaaagtacg cctgtaatgc accggcgtgt cctaattcct
     3901 tatgcttgta ctcccattca agattcgaga cgatttcgcg cagcgctttg gattgttcag
     3961 catcgatgta cagcgccaga gagcggatat ccaccaggct gggtaacaag acttgctgcg
     4021 cttccgggtt gatccaggat gtggcaaacg ccgcatcgaa ctccaataca tagcccgcga
     4081 tcgaaacggg tgagggtact gaattcaggt cggcaggagt cgagtcaccg aaaatccatg
     4141 aatggactcg acccggagca agagaaaaga tcgcgccttc gcgaaccggg tagatttccc
     4201 agtctattac gtgtattccg cgtccgagtg ttacccacac gatctgataa caatcgtggc
     4261 ggtgccgtgc gagcagttta gatcgcggct tgtcctccag acgcgattat tcgaaatgct
     4321 tcgatggtag ggattcagtc ccgttgatcg aataacttgc gatgctgctg aacttcgagc
     4381 gtctattttg tttcatgcct tcaaattcat gtagggcgtg tcatcaatca agccaaatca
     4441 ccgaggcgac gcaatagatg gcgcccagga agttgcgagc ggtcttgtcg tagcgggtgg
     4501 cgatacagcg gtactgctta agttttgcga agaagttttc gatcaagtgg cgcgctttgt
     4561 agaggtggtg gtcgcactca cgtggcggtt tgcgattggc accacgctgc ggaatcacaa
     4621 tttccttgcc ggccttgcgc agtggctcga tcactcgctg gtgtgcgtca taggccttgt
     4681 cagcgatgac gacttgcgct ggcgtgtcct tgagcaaaac atcggcgccg tccagatcgt
     4741 gcgcctgccc aggcgtgagg tgaaacccgg tcggattgcc gagc
//
LOCUS       DASWQN010000474         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1233144, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000474 DASWQN010000000
VERSION     DASWQN010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233144"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VGC82_07760"
     CDS             <1..317
                     /locus_tag="VGC82_07760"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423202.1"
                     /translation="VQRPDIQALMKKVEIVTTSAYDPELPGAAAHDQVTVTLANGQTL
                     EGEPVARATGHPSRPLTDQQIFEKFADCLDAGESPIPADVLFERLSAIQSINARDLTA
                     AV"
     gene            340..1353
                     /gene="dctP"
                     /locus_tag="VGC82_07765"
     CDS             340..1353
                     /gene="dctP"
                     /locus_tag="VGC82_07765"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY0423203.1"
                     /translation="MLAGRRIILGSALAAPFVRTARADPIKLRVSLDTAPGHGRTLSI
                     ADFLKKLEAASQGQIAPQLFDSGQLCPDQEVIKALVLGKVEMAVPGTWLVAAYVPEAD
                     LGQLPIFYGQPEEITHRAIDGIPGDMVNEQVSKKLRVTIPGRWIDLGFVNWYSARKPL
                     NTLRDLKGLKIRNSGGFAQPWRAQFFGGIPNVTPWPDVPLELSQGTFEALQTSSESCA
                     SAKLWDSGLRYGLIDHQNMGDYIPMFSESFWAILSPSLKSLVTDLWAANIGTYRTNMA
                     AAQDKAEKELKVRGVKIVVVPPEELAEQRKRMLDGQEHVAREMKISPDILARINELVA
                     AAN"
     gene            complement(1374..>1537)
                     /locus_tag="VGC82_07770"
     CDS             complement(1374..>1537)
                     /locus_tag="VGC82_07770"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEY0423204.1"
                     /translation="TSEDKAFFMTRTEVHCADCGGHLGHVFPDGPKPTGLRYCMNGVA
                     MAFKPADKP"
BASE COUNT          308 a          484 c          470 g          275 t
ORIGIN      
        1 tcgtccagcg cccggatatc caggcgctga tgaagaaggt ggagatcgtc accaccagcg
       61 cctatgatcc ggaactgccg ggtgccgccg cgcacgacca ggtcacggtg acgctggcca
      121 atgggcaaac tttggaaggc gaaccggtcg cacgcgcgac tggccacccg tctcgtccgc
      181 tgaccgacca gcagatcttc gagaagttcg ccgactgcct tgatgccgga gaatcgccaa
      241 tcccggccga cgtgttgttt gagcggctgt cggcgatcca gtcgatcaac gcgcgcgact
      301 tgaccgcggc agtatgatcg ccgcgatggg gatttgatcg tgctggcggg gcgccggatc
      361 attctcggtt cggcgctcgc cgcgcccttc gtacggacgg cgcgcgccga tcccatcaag
      421 ttgcgggtgt cgctggatac cgcccctgga catggccgca cgctgtcgat cgccgatttc
      481 ctgaagaaac tggaagccgc gtctcaaggg cagatcgctc cgcaactttt cgacagcggc
      541 cagctttgtc ccgaccagga ggtgatcaag gccctggtcc tgggaaaggt cgaaatggcg
      601 gtgccgggta cgtggctggt cgctgcctac gtgcccgagg ccgacctggg tcaactgccg
      661 attttttacg gccagccgga agaaattacc catcgtgcca tcgacgggat tcccggcgac
      721 atggtgaacg agcaggtcag caagaagctc cgggtcacca ttcctggacg ctggatcgac
      781 ctcggcttcg tcaactggta cagcgcccgt aaacccttga atacattgcg ggatctgaag
      841 ggactgaaga tccgtaactc cggcggcttc gcccagccct ggcgggcgca attcttcggc
      901 ggcatcccca acgtcacgcc ctggccggac gtcccgctgg agctgtcgca gggcaccttc
      961 gaggcgctgc agacctccag cgaaagctgc gccagcgcca aactgtggga ttccggcctg
     1021 cgctacggcc tgatcgacca tcaaaacatg ggcgactaca tcccgatgtt tagcgagagc
     1081 ttctgggcca tcctcagtcc gtcgttgaaa agcctggtca ccgatctctg ggccgccaat
     1141 atcggaacct accgaaccaa catggctgcc gcacaggaca aagccgagaa agagctcaag
     1201 gttcggggtg tcaaaatcgt ggtcgtgcca ccggaggaac tggccgaaca gcgcaaacga
     1261 atgctggacg ggcaggaaca cgtcgcgcgg gaaatgaaaa tttcacccga catcctggcg
     1321 cgcatcaatg aattggtcgc cgccgcgaac taaccgcagt ggtgcctcag cgactacggt
     1381 ttgtccgccg gtttgaacgc catcgccacg ccgttcatgc agtagcgcaa cccggtcggc
     1441 tttgggccgt cgggaaatac atggccgaga tggccgccgc aatccgcaca atgaacctcg
     1501 gtgcgggtca tgaaaaacgc cttgtcctcc gacgttc
//
LOCUS       DASWQN010000475         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2156623, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000475 DASWQN010000000
VERSION     DASWQN010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156623"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VGC82_07775"
     CDS             complement(<1..435)
                     /locus_tag="VGC82_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0423205.1"
                     /translation="MPDAAVSTTDLHYLSISDAARLIEQRRLSPVELTQAYLDRIQTF
                     DPQLNAYLLVVADRALAQARAAEADIAAGRYRGPMHGIPFALKDIYCTAGIRTTCHSR
                     TLADYVPAFDATTVAKLYDAGAVLLGKLSTHEFAHGGPSSDLP"
     gene            705..1136
                     /locus_tag="VGC82_07780"
     CDS             705..1136
                     /locus_tag="VGC82_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423206.1"
                     /translation="MIWVGGIVLAVALYLVGPDRFFDACLNLIDSIDAAFRNMVAELG
                     MKTYGVVRALALAIYVVFAVLAVLASQRGQRGFWPLIVVSALFMTLIWRPFGVYPAPL
                     NRWLAALILVVIAAVIMSQRLTVKPLRRNGSLPPYPPGHVP"
     gene            1133..>1878
                     /locus_tag="VGC82_07785"
     CDS             1133..>1878
                     /locus_tag="VGC82_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0423207.1"
                     /translation="MTQDTLAPDGATGPQKAAKAVLALSLTLLGAWTLHRYIPALIWA
                     PILAIAVWPLYQRSIRRFPPGRHNILLPTVFTLALGLMFIVPLGLVGVQAGKEVRGIY
                     ETVDKARTDGIPPPEWLGHLPAGGSQAMNWWNENLSNPDSASALLQKARESEFVANGR
                     HLGGEIVHRFVLFGFTLVTLFFLFRDGDRLTEQMMRASARAFGPAGERIGRQMIASVH
                     GTVDGLVLVGLGEGFILGVGYLIAGVPHPTL"
BASE COUNT          314 a          587 c          611 g          366 t
ORIGIN      
        1 cggcaggtcg gacgatggac cgccgtgggc gaactcgtgc gttgacagct tgcccaacag
       61 caccgcgccg gcgtcataca gtttcgcgac ggtcgtcgca tcgaacgcag ggacgtagtc
      121 cgctaacgtg cgcgagtggc acgtcgtccg gatgccggcg gtgcagtaaa tatccttcaa
      181 agcgaagggg atcccgtgca tcggcccgcg atagcgaccg gccgcgatgt ccgcttcggc
      241 cgcccgcgcc tgggccagcg cccgatccgc gacaaccagc agataggcgt tcaactgcgg
      301 atcgaatgtc tggatccggt ccagatacgc ctgcgtcagt tcgaccggcg acaggcgccg
      361 ctgctcgatc agccgagcgg catcggaaat cgaaagataa tgaagatcgg tcgtggaaac
      421 ggcggcatcg ggcatggcag gtggctcccc gtgtggataa gcgctccgtc tccttggtcg
      481 cgttccttgg tcgcggtcga cgccgcgtga agcgggagtt tcaccgatcg cgtcacatgt
      541 gcaagaggtt ggcttgtgcg gacggccggc agccaagaaa tgccgggccg cggataacgc
      601 tgtcacgatc ccgtcccccg atgtgcagtg ccggccggaa tcgcgtaaaa ggctgatgtc
      661 caccatcgaa caaaggcggc agcgtgcggc gagaacaaat tttgatgata tgggtcggcg
      721 gcattgtgct ggccgtggcg ctatacctgg tcgggccaga ccggtttttc gacgcctgtt
      781 tgaacttgat cgacagcatc gacgctgcat ttcgtaacat ggtcgcagaa ctggggatga
      841 aaacctacgg cgtcgtcagg gcgctggcgc tggcgatcta cgtcgtcttc gcggttctgg
      901 cggtactggc atcccagcgg ggccagcggg gcttctggcc gctgattgtc gtttcggcgc
      961 tattcatgac actgatctgg cggccgttcg gcgtttatcc cgctccactt aaccgctggc
     1021 tggcggccct gatcctggtc gtcatcgccg cggtgatcat gtcgcagcgg ctgaccgtga
     1081 aaccgctgcg tcgcaatgga tcgctgccgc cctatccgcc cgggcatgtc ccgtgacaca
     1141 ggacacgctg gcgccggacg gagccaccgg cccgcaaaag gcggcaaagg ctgtactggc
     1201 actgtccctg acgctgctgg gcgcgtggac cctgcaccgc tacatcccgg cgctgatctg
     1261 ggcaccgatc ctcgcgattg ccgtttggcc gctgtatcaa cgcagcattc gccggtttcc
     1321 gcccggccgg cacaacatcc tgctgccaac cgttttcacc ctggcgctcg gcctgatgtt
     1381 catcgtcccc ttggggctgg tcggggttca ggccgggaag gaagttcgcg ggatatacga
     1441 aaccgtcgac aaagcccgca ccgacggcat tcccccgccc gagtggctcg gtcaccttcc
     1501 cgccggcggt agtcaggcta tgaactggtg gaacgagaac ctgtccaatc ccgactctgc
     1561 gtcggcactg ctgcagaaag cacgggaatc ggagtttgtc gccaatggac gccatctggg
     1621 cggtgagatc gtccatcgct tcgtgctgtt cggctttacc ctagtgacgt tgttctttct
     1681 tttcagggat ggcgaccgcc tgaccgagca aatgatgcgc gccagcgccc gggccttcgg
     1741 gccagcgggc gagcgcatcg gccggcagat gatcgcctcg gtccatggca cggtcgatgg
     1801 gcttgtgctg gttggactgg gcgagggctt catcctcggc gtcgggtatc tgatcgccgg
     1861 cgtgcctcat cccacgct
//
LOCUS       DASWQN010000476         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2420344, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000476 DASWQN010000000
VERSION     DASWQN010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2420344"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1033)
                     /locus_tag="VGC82_07790"
     CDS             complement(179..1033)
                     /locus_tag="VGC82_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0423208.1"
                     /translation="MFDPVHLESFLAVAQTRNFTEAGRRLGLQQSTVSQHIRKLEESA
                     GRRLFVRDTHRVTLTADGEAMTGFAQTILDTSQRARRYFAGSQLRGRVRFGASEDFVS
                     TRLPEVLREFVRQHALVDLELKVGVSATLYELLDAGELDLVLAKRRAGDDRGRLVWRD
                     RLTWVGAPGIQVDAPVPLILFNAPSITRTVALEALERAGLPWRIACTSGSLSGLRAAC
                     LAGLGVTVHARGLTPEGLVEMPVTFGLPTLGDVEFVVMGVRDGLVGPAAALATSILAN
                     GDRLQRSG"
     gene            1274..2281
                     /locus_tag="VGC82_07795"
     CDS             1274..2281
                     /locus_tag="VGC82_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEY0423209.1"
                     /translation="MSPKRILTRLPFDPYIAAILVMVCLASLLPVYGNGAVAASYITT
                     AAIALLFFLHGARLAPRAALAGARHWRLHVVVLGSTFLLFPALALGARALFPHLLTPP
                     LWTGLILLSVLPSTVQSSIAFTSIARGNVPAALCAATASNLLGMALTPLLAGLLLSAH
                     GGFSIKGAGDIVVQLLLPFAAGQLARPLIGAWAQRHKKLLGLVDRGSILLVVYVAFSE
                     GVTQGIWHQLGVADLGRLLAVDAALLAVVLVITTFGSRLLGFSRADEITVVFCGSKKS
                     LASGLPMATVLLAGQSVGLIVLPLMLFHQIQLMVCAALAKRYANAEAGVAMAGLAQAA
                     R"
     gene            2355..3161
                     /locus_tag="VGC82_07800"
     CDS             2355..3161
                     /locus_tag="VGC82_07800"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0423210.1"
                     /translation="MIKRLVQGAIGWDHKTERDRYLFNYRVSSLLAQAISLLPVPMWR
                     LFLLFGSDKHRPGWHSYGHTYGALLRRWKYRPVKLLEIGIGGYRGSLGGRSLLAWQAF
                     FPFGTIVAADIVPKQDLGGARRRIVQLDQSSEADLGALCAREAPFDIVIDDGSHFNAH
                     QILTFQQVWTSLKDGGVYVVEDVQTSFWPGVVAGVQWDGADIGDPAFGQTCYGYFLEL
                     AKCVNHAEFRPSVQADTNLLRFAQQIKRITFEHNLIILLIGSNTETTAFS"
BASE COUNT          555 a         1026 c          963 g          656 t
ORIGIN      
        1 ctggccatga aacctcagac cgttgctaac cgcctctagc atgaatctcc cgaagcgagg
       61 caattgtcta acgcaagtcg aagcggtgcc aggcatctct caatcagaaa gcgatatcct
      121 gcagcgacgt gtcccacggc atctcttcat gataacgggc gctttcttcg ggtttatttc
      181 agcctgaccg ctgcagacga tcgccgttcg cgaggatcga ggttgccaag gccgccgccg
      241 ggcccacaag tccatcccga acgcccatga ccacgaattc cacatccccg agtgtaggca
      301 gaccaaaagt gacgggcatt tccaccaggc cctccggcgt caggccgcgc gcatgcacgg
      361 taaccccaag gccggccaga catgccgccc gcaaaccgct cagactcccg ctggtgcagg
      421 caatccgcca tggcagcccc gcgcgctcca acgcttccaa cgccaccgtg cgggtaatac
      481 tgggtgcatt gaacagaatc agcggcaccg gcgcatcgac ctgaatcccg ggcgctccca
      541 cccaggtcag gcggtcgcgc cataccagcc gtccccgatc atcgccggca cgtcgtttcg
      601 ccagcaccag atccaattcc ccagcgtcca gaagttcata cagggtcgcg ctgacgccta
      661 ccttcaactc cagatcgacc agggcatgct ggcgcacgaa ttctcgcaac acctccggca
      721 gccgtgtgct gacaaaatcc tccgacgcgc cgaaccgcac ccggccgcgc aattgagatc
      781 cggcgaagta gcgccgggcc cgctggctgg tatcaaggat ggtttgcgcg aaccctgtca
      841 tcgcctcccc atccgccgtc agcgtgacgc ggtgggtgtc gcggacgaac agcctgcgcc
      901 ccgcgctctc ctccagtttg cggatatgct gactgaccgt ggattgctgc agacccagcc
      961 gccgccccgc ctcggtaaag ttgcgggttt gggcgacggc aaggaagctc tcgaggtgga
     1021 cgggatcgaa catgaaggtg cctcagttca ccgccggcat tcactccggc gcggcgccgt
     1081 gcgcatctgt ccgatgtcct ccgcagcgaa gcggatgtag cgagcggcat ttttggctga
     1141 gcgtgtgatg gcggcggcgt tccaggcatt gttatcgcga atcatgatga caataacctt
     1201 gactaggctg gttcacaata acccgacgcg ggtgtaactc ggcggcatcg cataaagcca
     1261 gaaggatcgc gcgatgtctc cgaagcggat actgacccgg ctaccgttcg acccgtatat
     1321 cgcggccatc cttgtaatgg tttgcctggc gtcactgctg ccggtgtacg gaaacggcgc
     1381 cgtggccgcc agctacatca ccacggctgc gatcgcgctg ctgttcttcc tgcacggcgc
     1441 gcgcctggct ccccgagccg cgctggccgg cgcacgacat tggcgcttgc acgtagtggt
     1501 gctgggctcg accttcctgc tctttccggc acttgcactt ggggcccgcg cgctgtttcc
     1561 tcatctgctg acgcccccgc tttggactgg tctgatcctg ctgtctgtcc ttccctcgac
     1621 agtccaatcg tccattgcct ttacctccat cgcccgcggc aacgtaccgg ccgccttatg
     1681 cgccgcgacg gcatccaacc tgctcggcat ggcgctgacc ccgttgctgg ctgggttgct
     1741 gctcagcgcg catggcgggt tctcgatcaa gggcgctggc gacatcgtcg tgcaactgct
     1801 gctgccgttc gccgcgggcc agctcgcccg tcccctgatc ggcgcttggg cgcagcggca
     1861 caagaagctg ctcggcctgg tcgatcgcgg ttcgatcctg ctggtcgttt atgttgcgtt
     1921 cagcgagggc gtgacccagg gcatctggca ccaacttggc gtcgccgacc tcgggcggct
     1981 gctggcagtc gatgccgctt tgctcgctgt tgtcctggtg atcaccacct tcggcagccg
     2041 cctgctgggt ttctcgcgtg ccgatgaaat caccgtcgtc ttctgcggct ccaaaaagag
     2101 cctggccagc ggcctgccaa tggcaaccgt tctattggcc ggccagtcag tcggcttgat
     2161 cgtgctgccg ctgatgctgt tccaccaaat ccaactgatg gtctgtgccg cgttggctaa
     2221 gcgctatgcg aatgccgagg cgggcgttgc catggctgga ttggcccagg cagcgcggta
     2281 gcgcttgggc gccattttat ttgggtcacc agcgtaattt gcctagactt gcatcgagcg
     2341 gatggggtct gggcgtgatc aagcgattgg tgcaaggggc gataggctgg gaccataaga
     2401 ccgagcggga ccgatatctg ttcaactacc gtgtatccag tcttctggcg caggcgattt
     2461 ccttgttgcc ggtgccgatg tggcggctgt tcctgctgtt cgggtcggac aagcaccgac
     2521 cgggctggca tagttacggg cacacctacg gagcgttgtt gcgccgatgg aaatatcgcc
     2581 cggtgaagct gctggagatt ggcatcggcg gctatcgcgg ctccctcggc ggccgatcct
     2641 tgctggcctg gcaggcgttt ttccccttcg gaacgatcgt ggcggccgat atcgtgccga
     2701 agcaggacct cggtggcgcg cggcggcgca tcgttcaact cgatcagtca tccgaggcgg
     2761 atcttggcgc gctatgcgcc agggaggcgc cgtttgacat tgttatcgac gacggttcgc
     2821 atttcaacgc ccaccagatc ctgacgtttc aacaggtttg gacatcgttg aaagacggcg
     2881 gagtttatgt ggtcgaggat gtgcaaacgt cgttctggcc tggcgtcgtg gcgggggtac
     2941 agtgggacgg cgccgacatc ggggatcctg ccttcggcca gacctgctac ggctatttcc
     3001 tggaactggc gaaatgcgtg aaccacgccg agtttcggcc atccgtgcag gccgacacca
     3061 accttctgcg ctttgcccag caaatcaaac ggattacctt cgagcacaat ctgatcattc
     3121 tgctgatagg cagtaatacc gaaacaacgg ctttttcttg aagaaccaca gccgaagcct
     3181 gaaggggtcc ctgtgccgtg
//
LOCUS       DASWQN010000477         5168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_177551, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000477 DASWQN010000000
VERSION     DASWQN010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177551"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..376
                     /locus_tag="VGC82_07805"
     CDS             107..376
                     /locus_tag="VGC82_07805"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0423211.1"
                     /translation="MLVVKQAISLTECNVVWIQTIAHDVEHGVGLRELRVRGEAGNFR
                     VIYIAALADAVYVLHAFQKKTQQTARQDLNLVAARLRQIRKGRAG"
     gene            373..684
                     /locus_tag="VGC82_07810"
     CDS             373..684
                     /locus_tag="VGC82_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY0423212.1"
                     /translation="MTDEPVQIFAPVQIFASVWDALEDTPEEAAHLRLRSELAMALQD
                     AVQAWHLTQAQAADRLGVTQPRLNDLRRGRIARFSLDALVGLAERAGLAVRMEITRET
                     A"
     gene            complement(733..1446)
                     /locus_tag="VGC82_07815"
     CDS             complement(733..1446)
                     /locus_tag="VGC82_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HEY0423213.1"
                     /translation="MNAQRPPIDPIRTDGYSFVRAPEMRASLEQSGSLADWAAFAASW
                     DDLGPDTYMADGGRYRKRRHAVFAAGPREIVRQPAQPHYQSRDYNTLNGGIERWFEPV
                     SEPIGAGPSMTTILKFCRSTFGPLSPDTHAWHVEIHQFRIEARSGQQGRPTPEGMHRD
                     GVDYVLVLLVNRTNIASGVTSVHDLTGKALGEFTLTQPFDAALVDDARVMHGVTPVEP
                     LDAAEPGFRDVLVVTFRRA"
     gene            complement(1421..3778)
                     /locus_tag="VGC82_07820"
     CDS             complement(1421..3778)
                     /locus_tag="VGC82_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY0423214.1"
                     /translation="MIRLVLILLCAIVVCVPTPSAAQKPNPSPPPAISADQARAALDV
                     LNDPAKRATFAATLNAIVKAQPGPATAAEPAKPAMAEITLAPDSLGAQVLLSASAFVN
                     RVGNQAVDALDAVQSLPLLYGWAVVMATNPVARDLLADVAWRVVLVVAGAAAVEYSLR
                     RVLRRPIRALEGHAPLVRPIEHEDPLPPAGTPDDAVARAEAGESEAPAAARRARPSAW
                     TLLRRVPLVLARLFLELLPVFAIVVVGHLLTGSSIGGQTISRLIILAVVDAYAICMAL
                     LCVARMLLSPDESRLRLFHLTDAAAVYLTRWTRWLILIAVFGYVIGEVGLLLGLSEIA
                     HDALQKAFGLVLHVSLAFIVVNKRRAVRHWLRAPKDSTGPLATLRNRFARIWHWVALF
                     LLAAGWIIWAVEVPHGYVAVIHYFIVTALVLIGARFVLLLLLGVVDRVMRPAPDTPLL
                     YPGMHARLRIYHPTVTAALRLTIYLLCILGLLQIYGLNTFVWLLSSALGLRVLSASGT
                     LLLTIVLAFAAWEAINGGIQQHLDRLQRDAQLAKSARLRTLLPLVRTTLLITIAIVAG
                     LMILSEIGINIAPLLAGAGIVGVAIGFGSQKLVQDLITGIFLLLENAMQVGDSVTVSG
                     LSGVVEALSVRTIRLRAGDGSVHIIPFSSVTSVTNVNRGLGNAAINVSVAFDEDTDRV
                     AAELTAIVAGMRSDPTLSAQMLSDLQLWGVDKVDGASVTVAGQVVCTDSGRWAVQREF
                     NRRMKRRFQELGIRIFNPVQTITMSVPVQTPIQEGGEHERAAAAN"
     gene            complement(3869..4702)
                     /locus_tag="VGC82_07825"
     CDS             complement(3869..4702)
                     /locus_tag="VGC82_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0423215.1"
                     /translation="MSFLTEPEPERGARLPVLPGISRIVANNPGPMTYFGTNTYLIDT
                     PEGTAVLDPGPDGHPEHVEAIMRHTGGQIAFIMVSHTHHDHVGAVPALQAATGAPTVG
                     FRQSAIEAFAPDIKLDHESTFAGMQALHTPGHASDHLCFARVSADGAKVLFSADHVMS
                     WSTSIVSPPGGNMRDYFASLNLLLRRDDEIYLAGHGPLLREPRNLVRAMLAHRMLREQ
                     AIAQKLADGLSDTYTIMDALYSQLNPRLRKAAERNVLAHLLKMEAEGKARRDGEVWRA
                     A"
     gene            4881..>5168
                     /locus_tag="VGC82_07830"
     CDS             4881..>5168
                     /locus_tag="VGC82_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423216.1"
                     /translation="MAKGLLFASFDFSTAHEDEFHDWYDREHIPERLRVPGFFNAERW
                     IGEENPKIHVATYDLDSIAVLDTPPYRAVGGENQTAWTRRVTGMSRRIMRFV"
BASE COUNT         1031 a         1663 c         1623 g          851 t
ORIGIN      
        1 tcggcagtac cgggtaaacc tactatatcc gcttggtgta aaatgacgat gaagccagtc
       61 gcttttgccg gtagtgcatt ggatggttta agaaactttc cgatcaatgc tcgtcgtgaa
      121 gcaggccatc agcctgaccg agtgcaacgt ggtctggatc cagacgattg cacacgatgt
      181 cgagcacggc gtcggccttc gagagctccg ggtccggggt gaggcgggca atttccgcgt
      241 catctacatc gcagcgctgg ccgatgccgt ctacgtattg cacgccttcc aaaagaagac
      301 gcagcaaaca gcaaggcagg acctgaattt ggtagccgcc agattgcggc aaattcgcaa
      361 aggaagagca ggatgaccga cgaacctgtg cagatctttg cgcccgtgca gatcttcgca
      421 agtgtgtggg acgctttgga ggacacgccg gaggaagcag ctcatttgcg gctgcgttcg
      481 gagctggcca tggccttgca agacgcggtg caggcatggc acctgaccca agcccaggct
      541 gccgaccgat tgggagtgac gcagccgcgt ctcaatgatc tccggcgggg ccggatcgcc
      601 cgcttcagcc tggatgccct ggttgggctg gcggaacgtg cgggcttggc tgtccgcatg
      661 gagatcaccc gagaaactgc ctaatccccg gatgatggtg caggctggag ggccggccga
      721 gttgccacgc catcaagccc gccggaacgt taccaccagc acatcccgga atcccggttc
      781 cgccgcgtcg agcggctcca ccggcgttac cccgtgcatc acccgcgcat cgtccaccaa
      841 tgcggcatcg aacggctgcg tcagggtgaa ctcgcctaaa gccttgcccg tcagatcgtg
      901 gaccgaggtc acgccgctgg caatgttggt gcggttcacc agcaacacca gcacgtagtc
      961 caccccgtcg cgatgcatgc cctccggggt cgggcggccc tgctgaccac tgcgagcctc
     1021 gatgcggaac tgatgtatct ccacatgcca cgcatgcgta tccggactga ggggcccgaa
     1081 cgtgctccgg caaaacttca ggatcgtcgt catcgacggc ccggcgccga tcggttcgga
     1141 caccggctcg aaccagcgct cgatcccacc gttcagcgtg ttgtagtccc ggctttgata
     1201 atgcggctgc gccggctggc ggacgatctc ccgggggccg gccgcgaaca ccgcatggcg
     1261 acgctttcgg tagcggccac catcggccat gtaggtatcc ggccccagat cgtcccaact
     1321 cgcggcgaac gcggcccagt cggcaagcga accggactgt tccagcgagg cgcgcatctc
     1381 aggcgcccgg acgaaactgt atccatcggt ccggatcgga tcaattggcg gccgctgcgc
     1441 gttcatgttc tcccccctcc tggatagggg tctggaccgg gacggacatg gttatcgtct
     1501 gcaccgggtt gaagatcctg atcccaagct cctggaaccg ccgcttcatg cgccggttga
     1561 actcgcgttg cacggcccaa cggccagaat ccgtgcagac cacctgaccg gcaacggtca
     1621 ccgacgctcc atccacctta tcgacccccc aaagctgcag gtcgctcaac atttgcgcag
     1681 acaaagttgg gtccgagcgc atgcccgcga cgatcgccgt cagttccgcg gcgacgcgat
     1741 cggtgtcctc atcgaacgcc acgctgacat tgatggcggc gttgcccagc ccgcgattca
     1801 cgttggtgac gctggtgacg gaactgaacg gaatgatgtg gaccgacccg tcgccggccc
     1861 gcaggcggat ggtgcgcacc gacagcgcct cgaccacgcc ggacaggccg gatacggtta
     1921 ccgaatcgcc gacctgcatg gcgttctcca gcagcaggaa gatgccggtg atcaggtcct
     1981 gcaccagttt ctgcgaaccg aagccgatcg ccacgccgac aatgccggca ccggccagca
     2041 aaggcgcgat gttgatgccg atctcgctga ggatcatcag tcccgcgacg atcgcgatgg
     2101 tgatcagcaa cgtggtgcgc accagtggca gcaaggtccg aagccgggcc gatttcgcca
     2161 gttgcgcgtc gcgctgcagg cgatccaggt gctgctggat gccgccgttg atcgcctccc
     2221 acgcggcgaa cgccagcacg atcgtcagca gcagggtgcc gctcgccgaa aggacccgaa
     2281 gccccagcgc gctggaaagc agccagacga aggtgttcag gccgtagatt tgcagcaggc
     2341 caaggatgca cagcaggtag atcgtgaggc gcagcgccgc cgtgacggtc gggtgataga
     2401 tgcgcagcct ggcatgcatc ccgggataca gcagcggcgt atcgggagcc ggacgcatca
     2461 cccggtccac aacacccagc agcagcaaca ggacaaaccg ggcgccgatc agcaccagcg
     2521 cggtgacgat gaaatagtgg ataaccgcaa catagccatg cggcacttcc accgcccaga
     2581 tgatccagcc ggcagccagc aggaacaatg cgacccagtg ccagatccgg gcgaagcggt
     2641 tgcgcaatgt ggcgagcggg cctgtgctgt ccttcggcgc gcgcagccaa tgccgtaccg
     2701 cccttcgttt gttcacaacg atgaatgcca gggaaacgtg cagcaccagg ccaaacgcct
     2761 tttgcagcgc gtcgtgggca atttcggaca gaccgagcag aaggcccact tcgcctatca
     2821 cgtagccgaa cacggcgatc aggatcagcc accgtgtcca gcgcgtcagg tacacggccg
     2881 ccgcgtcggt cagatgaaac agccgcaggc gcgattcatc gggcgacaac aacatgcgcg
     2941 ccacgcaaag cagcgccatg cagatggcgt aagcgtccac gaccgccagg ataatcagcc
     3001 ggctgatggt ttgaccgccg atactcgatc cggtgagaag gtgtccgacc acgacgattg
     3061 cgaacaccgg caacaattcg aggaacagcc gggcgaggac cagggggacg cggcgaagca
     3121 acgtccaggc cgagggacgc gcccgtcgcg cggcagccgg cgcctcgctc tcaccggcct
     3181 cggcccgggc gacagcgtca tccggggtcc cggcaggcgg caaaggatcc tcgtgttcaa
     3241 tgggccgaac gagtggcgca tggccttcca gggcgcggat cggacggcgc aggacgcgcc
     3301 gcaggctgta ttcgacagcg gctgcgccag ccaccacaag aactacacgc caggcgacat
     3361 ccgccagcaa atcacgcgca acggggttgg tcgccatgac cacggcccag ccgtaaagca
     3421 gcggcaggct ttgcaccgcg tccaacgcat ccaccgcctg gttgccgacg cggttgacga
     3481 aggctgaagc cgacagcagg acctgcgcac ccaggctgtc tggggcgagc gtgatctcgg
     3541 ccatcgccgg cttggccggt tccgccgccg tggccggccc cggctgcgcc ttcacgatgg
     3601 cattcaaagt cgcggcaaag gtcgcccgct tggccggatc gttcagcaca tccagtgccg
     3661 cccgcgcctg atcagcggag atggccggag gcggtgacgg atttggtttc tgcgcggcgg
     3721 acggcgtcgg gacgcaaacg acgatggcgc acagcaggat aagaacgagg cgtatcatcc
     3781 gcgggtacga ccacagatgc cgccgcccgg tcaacgcaat tccggctatc aaacctgctg
     3841 gcgaacgact tccggaatgc ccgccgcttc aggcggctcg ccagacttcc ccgtcgcgcc
     3901 gtgccttgcc ctcggcctcc atcttcagca aatgagccag cacattgcgc tccgctgcct
     3961 tgcgcagcct gggattcaac tgcgaataca gcgcgtccat gatcgtgtac gtgtccgaca
     4021 gcccgtcagc cagcttctgc gcgatcgcct gctcgcggag catccgatgc gcgagcatgg
     4081 cccggaccag gttccgtggt tcgcgcagca gcggaccgtg accagccaga tagatctcgt
     4141 catcacgtcg tagcaacaaa ttcaggctgg cgaagtaatc gcgcatattt ccgcccggag
     4201 ggctgacgat gctggtggac cacgacatga cgtgatcggc gctgaacagc accttggcac
     4261 cgtcggcgct tactcgcgcg aagcacagat gatcggatgc atgacccggc gtatgcagcg
     4321 cctgcatgcc ggcaaacgtg ctctcgtggt ccagtttgat gtcgggcgcg aaagcctcga
     4381 tagcgctttg gcggaagccg acggtcggcg ctccggttgc cgcctgcaaa gccggaaccg
     4441 ctccgacatg atcgtgatgg gtgtggctga ccatgatgaa cgcgatctgg ccgccggtgt
     4501 gccgcatgat cgcttccacg tgctcgggat gaccatcggg accagggtcg agcaccgccg
     4561 ttccctctgg ggtatcgatc aggtaagtgt tcgtcccgaa ataggtcatc gggccgggat
     4621 tattggcgac gatccggctg atccccggaa gcaccggcag cctggcgccg cgctcgggtt
     4681 ctggttcggt caaaaaggac atggttcaag gactccgttt aagctggctc gccggagcgc
     4741 tttgatcgcc tctccaacag ttccctgctt gaatgccgta aaccgtccag actggcaacg
     4801 caggcgcgtc ccgtgcaaag tgccgacagc aaggaaatta ccgagagcaa gaaagtgccg
     4861 gcagcaagga gggaatggaa atggccaaag gtctgctatt cgcgtcgttc gacttctcca
     4921 cggcgcacga agatgaattc cacgactggt acgaccgcga gcatattccg gaacggctgc
     4981 gggttcccgg cttcttcaac gcggaacgct ggatcggcga agaaaacccg aagatccacg
     5041 tcgcaacgta cgacctggat tcaatcgccg tcctggatac cccaccgtac cgggcagtcg
     5101 gcggtgagaa tcaaaccgcc tggacccggc gtgtcaccgg catgagcagg cggatcatgc
     5161 gtttcgtc
//
LOCUS       DASWQN010000478         5176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_46095, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000478 DASWQN010000000
VERSION     DASWQN010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46095"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VGC82_07835"
     CDS             <1..496
                     /locus_tag="VGC82_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423217.1"
                     /translation="HAADSRWGTAYSARPPVGGIVAARDKAIAFFSGFHARSAVDGFR
                     AGTGSAARCVRAFSAGSSHAAAGGGIARRCNTFRNRTWTFRNGNWQALPADVGNRGCA
                     CSTGTRFAVAAFAGDAASTGPGDAVSSDCGGAFSTHPSAADPSAAGPGNLGAAFGTPR
                     FDAG"
     gene            873..1157
                     /locus_tag="VGC82_07840"
     CDS             873..1157
                     /locus_tag="VGC82_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423218.1"
                     /translation="MASGRIEDARRLLQEAQLQLVFRPLDAAGEDQPSAGKRAADVAH
                     ALEAMGANDLALSRRYIDAALGNSPITAGGPPPQQSERRVSGYAPAYPGR"
     gene            1456..2286
                     /locus_tag="VGC82_07845"
     CDS             1456..2286
                     /locus_tag="VGC82_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY0423219.1"
                     /translation="MSQETAPMVLKRNGTGRSVRRIRSAAPAGAALMGLAVLAACSTV
                     PTLPPAPMEPEASTVTANLPPYRIQVGDVLEVRLLLNPELNEEVTVRPDGHISTTAIP
                     DALAYGMTPADLADQLRRGYSRDLQNPRVSIVVRSFAPTRVYVGGEVNTPGEFITVGP
                     SLSLSQALARAGGTRLSSDDTAVFVIRRGPGDKPQFLSTRWKAVRQGRDPEADIRLAP
                     YDVVYVPKLGIAEVYQFYNQYLQQFVNPSFGFSYLLNPGNNVVTSTGGAAATTAPVGR
                     "
     gene            complement(3191..3574)
                     /locus_tag="VGC82_07850"
     CDS             complement(3191..3574)
                     /locus_tag="VGC82_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423220.1"
                     /translation="MKYLASTVGFLALGAIVGSMSPRPVAAAPFCIQSQGIAPLCIYY
                     DALDCGHEAQRQGATCAANPAELRLTPGVGQYCVVTSSQISLCNYGDRVSCARDAVLQ
                     RGTCVDSPRTAPIRTPDPYSPVNGN"
     gene            3725..4030
                     /locus_tag="VGC82_07855"
     CDS             3725..4030
                     /locus_tag="VGC82_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423221.1"
                     /translation="MPGPAMVRGLDEAERRTLSEWLEAACTRGIDTVMDFSVREWNVA
                     GAAAIVGVFETDKNQASWLVVRDGSRWSLARCSDGCVSGGSRSLAHVLALIDADPAD"
     gene            complement(4163..>5176)
                     /locus_tag="VGC82_07860"
     CDS             complement(4163..>5176)
                     /locus_tag="VGC82_07860"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0423222.1"
                     /translation="VDRRKDAPRVESPAIVGDSPAMRQVASLVRRFEQTDEPVLITGE
                     SGTGKELLARAIHQGSRRAHGPFVALNCAAIPPGLVASELFGHERGAFTGAAARSLGQ
                     IEFANGGTLFLDEIGDMPIDLQGHLLRFLQEGQIRRVGGRETIGLDVRIVSATNVRLG
                     QAIAEGRFREDLFYRLNVLTLPVPPLRERPEDIAPLAHHFLGSAQRGFKRQVEGFSAD
                     AMAALRRYRWPGNVRELMSVVRRAVVVGDGPLIDSTMLIGLEDRPDPTGVPILLKPGS
                     AEERTALLATLERTGENITSAADALRVSRVTLYRMLRRHDIVLKRGLAPAPIARRPQH
                     ALG"
BASE COUNT          894 a         1684 c         1683 g          915 t
ORIGIN      
        1 ccacgccgcc gatagccgat ggggaactgc ctatagcgcc cggccccccg ttggcggcat
       61 cgtcgcagcc cgcgacaaag ccattgcctt tttcagcggc ttccacgcca gatctgccgt
      121 cgatggcttc cgcgccggca ccggaagcgc cgcccgctgc gtccgtgcct tctccgccgg
      181 cagcagccat gccgccgccg gcggcggcat cgcccgccgc tgcaatacct tccgcaatcg
      241 cacctggacc ttccgcaatg gcaactggca ggccctcccc gccgatgtcg gcaaccgagg
      301 ctgtgcctgc tccaccggca cccgcttcgc tgtcgccgcc ttcgcaggtg atgccgcctc
      361 caccggtcca ggcgatgccg tctcctccga ttgcggcggt gccttctcca cccatcctag
      421 cgccgccgac cccagcgccg ccggccccgg caaccttggc gctgccttcg gaacgccccg
      481 cttcgacgcc ggatgaggcg ccaccggcgc cggccgcgat gcccgcttca ccggctgtgc
      541 tgcccacaag gcatgtaccg gatcggcggg aagttgccgg ggaaccaggg ccgtccaaaa
      601 ccgagccgct caagaccgag ccgccgcagg ctgagccggt gaggccgccc ccgcaaaggc
      661 aggcgtcgcc aaagccgggg ccggcagttg tgtcgcagcc ttcgccacct cggccggatg
      721 gcgaggatgc tcaatcggtc ctcgcccggt tgcgtcagag cggacccgca tccgcgccac
      781 ctcaaccggc agatccactg ccggcacccg aaccgaggtt tcggccgcag ccttcgcccg
      841 cgttaccgaa gctgggtgcg gcgcgtgccg ctttggcgag cggccggatc gaggatgccc
      901 gccggctgtt gcaggaagcg caactgcagc ttgttttccg accgctcgac gcggccggtg
      961 aagaccagcc atcagcggga aagcgcgcgg ctgacgttgc ccatgcgctg gaagccatgg
     1021 gtgcaaacga cctcgcgctc agtcgccgct acatcgacgc cgcattgggt aattcgccca
     1081 taacggccgg cggtccgccg ccgcagcaat ccgagcggcg ggtgtcgggc tatgcccccg
     1141 cctatccagg acgatagaac ggtgggactg taaagtaggc ggcgcaccag cacgcaacac
     1201 ttgacgccgc ctttacgtaa ctggtgcatc cgatcgggct gctgtcgggc accggggact
     1261 ggcacatgat ttgctgtctt gatcttgtga tcagtccaac atgatcgcgg aaatagtcgt
     1321 atgatcgttt cacggggttt tcgtttggat tttggcgtaa tcggttccac acgctttcag
     1381 ggaaacctgc tgcccacgaa tatcgcccag ccgtcgagca tccgccgctg aacattccga
     1441 ttaagggttc aaaacatgag tcaagaaacc gctcccatgg tcctgaagcg taatggaacc
     1501 ggacgctccg ttcggcgaat tcgttcggcc gctcccgcgg gggccgcatt gatggggctg
     1561 gccgtgctgg cggcgtgctc gacggttccg acactgccgc cggcgccgat ggaacccgag
     1621 gcatcgacgg tcacggccaa cctgccgccg tatcgcattc aggtcgggga cgtccttgaa
     1681 gttcgcctgt tgctcaatcc ggaactgaac gaggaggtga cggtccgtcc cgacggccat
     1741 atctccacca cggcgatccc ggatgcgctc gcgtacggga tgacaccggc ggatctcgca
     1801 gatcaactgc gccgaggcta ttcccgggac ctgcaaaatc cacgggtgtc gatcgtggtc
     1861 aggtcgttcg cgccgacccg cgtttacgtc ggcggcgaag tgaacacccc cggcgaattc
     1921 atcaccgtgg gaccgtcctt gtccttgtcg caggcgttgg cgcgtgccgg aggcaccagg
     1981 ctcagcagcg acgatacggc ggtgttcgtc atccggcgcg gcccggggga caagccgcag
     2041 ttcctgtcca cgaggtggaa ggcggtgcgg caggggcgcg atccggaggc cgatatccgg
     2101 ctggcacctt acgatgtggt gtacgtcccg aaacttggga tcgccgaagt atatcagttc
     2161 tacaaccagt atcttcagca gttcgtcaat ccgagcttcg ggttctctta tctgcttaat
     2221 cccggcaata acgtggtcac gtcgacaggt ggcgctgcgg ccaccaccgc accggtcggg
     2281 cggtaacgga ctggcgccgg ccggcccggc gccacgctct gccttgcgtc aggcgcgcca
     2341 ctgcgaagtc ggacgcgatc gggtcatcgg cgccgctcat gtctatccga gatttgctcg
     2401 gaaacgcaga gcgcgccgag gatgcggaga ttgccgagac gtctttgatg cacctggcgt
     2461 tgtggcactt gtggttggtc cacgacgcag ttccaccttg cggttccgca accaacgttg
     2521 agaaattcga cagctaggcg cagccccgcg agcggtctca cggcgactgc gaaccggggt
     2581 ttcgtggatc cgcgggcatg attcggtcat gccggcgagg actggtaagg cggacctcaa
     2641 gcaggataaa ccgaggatga acacggttaa gttgggactg cgccctagtt aatcggtctg
     2701 aagcggtagt cgactcccgc gttgggtgga cgaccaacct ttgcagtatg tcgcggcccg
     2761 atccgaccag ccggtgtgcg catccggtag cattgcggcg gttcgcgttt gggtttgaaa
     2821 ggaagtcgtg ggtggccggt ccaagcccgg ccatgacacg gggtggctgg ccacgacacc
     2881 ggggtgctcc gtaccaccaa ccctggcgtg gcccggccaa gcctcgttat caccgcgggg
     2941 gctgctggcc acgatacccg gggtgctccg taccatcaac ttcggtgtgg cccgggcaag
     3001 cctcgttatc accgcagggg tggctggcca cgacaccggg gttgctcgtg caagcctgca
     3061 tcaatcccag ggggtggctg gccggaagcg ctctcgggtt cagcctgacc ctaccggccg
     3121 aaacgcttca ttgccttgtt tgccctcgcg actcacccgt attggaggtt ctgtccaggg
     3181 caagcgcgta ttagtttccg ttaaccggtg aataaggatc gggtgtgcgt atcggcgcgg
     3241 tgcgcggtga atcgacgcag gtgccgcgct gaaggacagc gtcgcgggcg caggaaacac
     3301 ggtcgccgta gttgcacagg gaaatctgtg aggatgtgac gacgcagtat tggccgacgc
     3361 cgggcgtcag cctgagctcc gccggattcg cggcgcaggt ggccccctgc cgttgtgcct
     3421 cgtggccgca atccagcgcg tcgtagtaga tgcacagcgg cgcgattccc tgactttgga
     3481 tgcagaaggg cgcagcggcg accggtcgcg gcgacatact gccaacgatc gcacccaacg
     3541 caaggaagcc aaccgtcgag gcgagatatt tcatggtcgc tcctgtagcg aggatggaag
     3601 ccgataaggg cgcaagcttc atgccagccc cgtcagggaa tcggaacata aggtcgccgg
     3661 atggcatggg ctttgcgctg ggttcggtgt tcgcttcaac accggccggg acgaggatat
     3721 ccgggtgcca ggaccagcaa tggtgagagg ccttgatgag gctgaacgcc gcaccttgtc
     3781 ggaatggctg gaggctgctt gcaccagggg catcgacacg gtcatggact tttccgtccg
     3841 ggaatggaat gtcgccggag cggcggcaat cgtcggggtg ttcgaaaccg acaagaacca
     3901 agcatcatgg ctagtcgtgc gcgacggttc aaggtggtca ctcgctcgat gcagcgacgg
     3961 ttgcgtatcc ggcgggtcga gatccctggc ccatgtgctt gccttgatcg acgcagatcc
     4021 ggcggactga ggacatgcgg gtcaggcctg tcgccctgtt cgagagcata atccatatcc
     4081 ctcgaatcat tccgccgggc gtgatcatgc cttggccgat cgcatgccct ggctgatcgc
     4141 atgccctggc cgagcgcatg ccctagccga gcgcatgttg gggccgtcgc gcgatcgggg
     4201 cgggcgccaa gccacgtttc aggacgatgt cgtgccggcg cagcatgcga taaagcgtca
     4261 cgcgagaaac ccgtagcgca tcggccgccg acgtgatgtt ttcccccgtc ctttccaggg
     4321 tcgccagcag tgccgttcgt tcttcggcgg aaccgggttt cagcaggatc ggcacgccag
     4381 tcgggtcggg ccggtcctcc aggccgatca gcatcgtcga gtcgatcagc gggccgtccc
     4441 cgaccaccac cgcgcggcgt accaccgaca tcagttcgcg gacgttgccg ggccagcggt
     4501 agcggcgcaa cgccgccatc gcatcggccg aaaacccttc cacctggcgc ttgaaaccgc
     4561 gctgcgccga acccaggaaa tgatgcgcaa gaggggcgat atcctcgggc cgttcgcgca
     4621 acggtggcac cggcagtgtc agcacgttca acctgtagaa caaatcttcg cgaaagcggc
     4681 cctcggcgat ggcctgaccc agacgcacat tggtcgcgga gacgatgcga acatcaaggc
     4741 cgatggtttc gcgcccgccg acgcggcgga tctggccttc ttgcaggaag cgcagcaagt
     4801 gaccttgcag atcgatcggc atgtcgccga tttcgtcaag gaaaagcgtg cccccgttgg
     4861 cgaactcgat ctggccgagg ctgcgcgcgg cggcgccagt gaatgctccg cgttcatggc
     4921 caaacaattc ggatgccacg aggccgggcg ggatcgccgc gcagttcagc gccacgaacg
     4981 gcccatgcgc gcggcgggag ccttgatgga tggcgcgcgc cagcaattcc ttgccggtgc
     5041 cgctttcgcc ggtgatcaat accggttcgt ccgtctgctc gaaccgccgt accagcgagg
     5101 cgacctgacg catggccgga ctgtcgccga cgatcgccgg cgattcaacg cgcggtgcat
     5161 ccttccggcg atccac
//
LOCUS       DASWQN010000479         3709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2553054, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000479 DASWQN010000000
VERSION     DASWQN010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2553054"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VGC82_07865"
     CDS             <1..1151
                     /locus_tag="VGC82_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0423223.1"
                     /translation="KKPFSALPFALARTQPSPPVIMPAHIAATDPYKQIPQVVGSGPF
                     RFEPSEYVSGSRVVFSKAGGYLPRTETPDFTAGAKRALVERIEWRIVPDASTAANALQ
                     AGEIDWLESPLPDLLPLLRGNKDVVVDRLDQYGLYPVARFNALQGPTARLGVRQAILA
                     AIDPVEVMQAVMGEDTTAYNAPVGTFLPGTPSASTAGMDRLGGKKSIEEIKAMLAAAG
                     YSGEKVVLLHPTDQPFYDAMTQVVAATLQKIGVTVDDQSMDFGTVVQRRASKEPIDKG
                     GWSIFCASFPAVDYLNPLAVPAARGNGGKAWFGWPDDAKLETLHDQWLDTADAAEQAR
                     LAAAFQQEVLTYAAYVPLGQYFPPTAWRRTVTGQLKGPVPVFWNIRKA"
     gene            1158..1535
                     /gene="crcB"
                     /locus_tag="VGC82_07870"
     CDS             1158..1535
                     /gene="crcB"
                     /locus_tag="VGC82_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEY0423224.1"
                     /translation="MGAWVAVALGGAIGSVGRFWLTGAMTALTGPRFPWGTLLINVLG
                     SLVIGLVAGFTLTPVRISVHPDIRIFLMTGVCGGFTTFSAFSLQTLELLQSGDTVAAF
                     GYAIGSVVLCVIATYAGWALGNL"
     gene            1654..2889
                     /locus_tag="VGC82_07875"
     CDS             1654..2889
                     /locus_tag="VGC82_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0423225.1"
                     /translation="MDFVIRNARIFRGSPGSGEAVVDIGIEAGRIVAIEANLVTDAPG
                     YDAAGHLACAGLIETHIHLDKTRIIDRCPPEDGRNANAVPRVAAVKHTFSEQDVYQRA
                     STTLENCIKHGTTRMRTHVELDGGVEMRSFDALEKLRRDYAWAIDIELCVFPQEGLTN
                     NKRADELLVQALKRGARVIGAAPNYDPDKSGQIHRIFDLAREYDVDIDMHLDSGNSPE
                     DMDIHLVCELTRKYGLGGRVAVGHGCKYSTLPPPDLRALATQIADAGVAVTVLPATDL
                     YMMGRNQDHSIQRGVANANVLTENGVNCSISSNNILNPFTPLGDGNLIRIANMQANVC
                     QIGESRRLRECFAMLTERSARLMNLKDYGIAIGNPADIVVFDAASPDEAIAEVRGPLV
                     VFKHGVQTVTRPRAELHRP"
     gene            2886..3188
                     /locus_tag="VGC82_07880"
     CDS             2886..3188
                     /locus_tag="VGC82_07880"
                     /inference="COORDINATES: protein motif:HMM:NF025688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HEY0423226.1"
                     /translation="MKLSDIAQVIRSKNAGPRRLTLDIMFGSDADYQRVVQAPALAAE
                     NIGRLYHVPADEVTIIPYPVGRAIKIVMARTIMAGDPGDYDVYGAQQHAPLLGIEI"
     gene            3229..>3709
                     /locus_tag="VGC82_07885"
     CDS             3229..>3709
                     /locus_tag="VGC82_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423227.1"
                     /translation="MSASGRVIAEFRRSPRSIRLLGASGQLGYGIPAASFNRGIDRHP
                     DLIGCDMGSIDIGPTYLGKGDMATAPEATRRDLRRVLQAARALDVPLVIGSAGSAGAG
                     PHLDATLAMVRNIARADGQRFRMAVMRADIPRPTLQGAVRAGKVAGFDGMPALTEEDV
                     "
BASE COUNT          662 a         1274 c         1158 g          615 t
ORIGIN      
        1 tgaagaagcc gttctccgcg ttgcccttcg ccttggcccg cacgcagccc agcccaccgg
       61 tcatcatgcc ggcgcacatc gccgccaccg acccgtacaa gcagatccca caggtcgttg
      121 gcagcggccc gttccgattc gagccgtcgg agtatgtttc cggcagccgg gtcgtcttct
      181 ccaaagcggg cggctatttg ccgcggacgg aaacccccga cttcaccgcc ggcgccaagc
      241 gcgctttggt cgagcgcatc gagtggcgta tcgtgcctga tgcatccacc gcggcgaacg
      301 ccttgcaggc cggagagatc gactggctgg aatcgccgct gccggacctg ctgccgctgc
      361 tgcgcgggaa caaggatgtc gtggtggacc ggctggacca gtacgggctg tatccggtcg
      421 cccgcttcaa cgcgctgcaa ggcccgaccg cgcggctcgg cgtgcgccaa gcgatcctgg
      481 cggcgatcga cccggtcgag gtcatgcagg ccgtgatggg tgaggatacc accgcctaca
      541 acgccccggt cggcaccttc ctgcccggca cgcccagcgc cagcaccgcc ggtatggacc
      601 gcctgggcgg caagaagtcc atcgaggaga tcaaggcgat gctggcagcc gccggctaca
      661 gcggtgagaa agtcgtgttg ctgcacccca ccgaccagcc gttctacgac gccatgaccc
      721 aggtcgtcgc cgccacgcta caaaagatcg gcgtcacggt ggacgaccag tcgatggatt
      781 tcggcaccgt ggtgcagcgc cgcgcgagca aggagccgat cgacaaaggc ggctggtcga
      841 tcttctgcgc ctctttcccg gccgtcgatt acctcaatcc gctggccgtc ccggccgccc
      901 gcggcaacgg cggcaaagcc tggttcggct ggccggacga tgcgaaactg gagacgctgc
      961 acgaccagtg gctcgacacc gccgacgccg cggagcaagc caggctggcc gccgcgtttc
     1021 agcaggaggt cctgacctac gccgcctacg ttccgctcgg gcagtatttc ccgccgacgg
     1081 catggaggcg gacggtgact ggccaattga aaggcccggt gccggtcttc tggaacattc
     1141 ggaaggcata gacaggaatg ggagcctggg tcgcggtcgc tttgggtggg gcgatcggca
     1201 gcgttggacg gttctggctt actggggcga tgacggcgct gaccggtccg cgcttcccgt
     1261 ggggcacgct gctgatcaac gtgctgggct ccctggtgat tggtctggtc gcggggttca
     1321 ccctgactcc agttcggatc agcgtgcacc ccgacatccg catcttcctg atgacaggcg
     1381 tctgcggcgg tttcaccacg ttctccgcct tcagcctgca aacgctggag ttgctgcagt
     1441 ccggcgacac tgtcgcagcc ttcggatacg cgatcggttc ggtcgtgctg tgcgtcatcg
     1501 ccacctatgc cggctgggcg ctgggcaacc tctgatttcc agtaaggccc ccggtcattg
     1561 cgacgaacgt ggcaacgcgg cattcttcca ccaccggacc gctttgccgc tgcgctcttc
     1621 gcaacgccaa acgtgtcgac ggagaggcac gccatggact tcgttatccg caacgcacgc
     1681 atcttcaggg gttcgccggg tagcggcgaa gcggtggtcg atatcggcat cgaggccggc
     1741 cgcatcgtcg cgatcgaagc caacctcgtg accgacgccc cgggttacga cgctgccggc
     1801 cacttggcct gtgccggcct gatagaaacc cacattcatc tcgacaaaac ccggatcatc
     1861 gaccgctgcc cgccggagga cggccgcaac gccaacgcgg ttccccgcgt cgccgcggtc
     1921 aagcatacct tctccgaaca agacgtttac caacgcgcca gcaccacgct ggaaaactgc
     1981 atcaagcacg gcaccacgcg catgcggacg catgtggaac tcgatggcgg cgtggaaatg
     2041 cgcagcttcg acgcgctgga aaaactgcgg cgcgactacg cctgggcgat cgacatcgag
     2101 ttgtgcgtct tcccacagga gggcctgacg aacaacaagc gcgccgacga attgctggtc
     2161 caggccttga agcgcggcgc cagggtgatc ggcgccgcac ccaattacga tccggacaaa
     2221 agcggccaga tccaccggat tttcgatctc gcccgcgagt atgacgtgga catcgacatg
     2281 cacctggatt ccggcaacag cccggaggac atggacatcc acctggtctg cgagctgaca
     2341 agaaagtacg gcctgggtgg acgcgtggcc gtgggccacg gctgcaagta ctcgacattg
     2401 ccgccgcccg acctgcgtgc gctggccacc cagatcgccg atgccggcgt cgcggtcacc
     2461 gttctgcctg ccaccgacct gtacatgatg ggccgcaacc aggaccacag catccagcgc
     2521 ggcgtcgcca acgccaacgt gctgacggag aacggcgtca actgctcgat ctccagcaac
     2581 aacatcctca acccattcac gccgctaggc gacggcaacc tgatccgcat cgccaacatg
     2641 caggccaatg tctgccagat cggcgaatct cgccgcctgc gcgaatgctt cgccatgctg
     2701 accgaacggt ccgcgcgact gatgaacctg aaggattacg gcatcgccat aggcaatccc
     2761 gccgacatcg tcgtcttcga cgccgcctcc cccgatgaag ccatcgccga ggtccgcggc
     2821 cccctggttg ttttcaaaca tggggttcaa acggtaaccc gcccaagagc ggagctgcac
     2881 cggccatgaa actgtccgat atcgcgcagg tcatccgcag caagaacgcc ggcccgcggc
     2941 ggctgaccct ggacatcatg ttcggctccg acgcggacta ccagcgcgtt gtccaggcgc
     3001 ccgcgcttgc ggcggaaaac atcgggcgcc tgtatcacgt gccggcggac gaggtgacca
     3061 tcatcccata ccctgtcggg cgcgcgatca agatcgtgat ggcgcgtacg attatggccg
     3121 gcgatcccgg cgattatgat gtttacgggg cgcagcaaca cgcgcccctg ctggggatcg
     3181 agatatgacg gcggccggta gaacttatga tccggggagc ccttttgcat gagcgcatcc
     3241 ggacgcgtca tcgccgaatt ccgccggtca cctcgttcga tccgcctctt gggagcttcc
     3301 ggccaactcg gctacggcat ccccgcggcg tcgttcaatc gcggtatcga ccgccacccg
     3361 gacctgatcg ggtgcgacat ggggtcgatc gacataggcc cgacctacct gggcaagggc
     3421 gacatggcga ccgcccccga agccacgcgc cgcgacctcc gccgtgtcct gcaagccgcc
     3481 cgcgcattgg acgtgccgct ggtcatcggc tccgcgggat cggccggtgc gggcccacat
     3541 ctcgatgcca ccctggctat ggtccggaac atcgcccgcg ccgacggcca gcgcttccgc
     3601 atggcggtga tgcgcgccga catccctcgt ccgacacttc agggcgctgt tcgcgccggc
     3661 aaagtcgccg gcttcgacgg catgcccgcg ctgaccgagg aagacgtta
//
LOCUS       DASWQN010000480         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1893538, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000480 DASWQN010000000
VERSION     DASWQN010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893538"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VGC82_07890"
     CDS             <1..469
                     /locus_tag="VGC82_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0423228.1"
                     /translation="PNWVMGAEAQYSWLPSSNNNGVLFPGGTLVTSNTDQLGSVTGRL
                     GYTWGPALLYAKGGYAWRDGNNLGVSVAGVPAAFTTNGSHKDGYTVGGGLEYMFAPNW
                     SAKAEYQYYNFGNTTFTTGPVDIAGRSFRNDEHTAKVGVNYRFGWGGAAASRY"
     gene            550..903
                     /locus_tag="VGC82_07895"
     CDS             550..903
                     /locus_tag="VGC82_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423229.1"
                     /translation="MRVAADILSAAKIIFAVHATFRHDALLLMSAGWVGHQIMRVFAL
                     GLIFSAAAFPCLAQTVLKSEPLMLAPYEVAFVQDSSCGAGKVRKVTGAIRGLQRRKAC
                     VTLAAEQASLVSATP"
     gene            complement(1003..1257)
                     /locus_tag="VGC82_07900"
     CDS             complement(1003..1257)
                     /locus_tag="VGC82_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423230.1"
                     /translation="MPYALFSNDAKLSKAYPTAADVWKIAQKSGLVVDVVSEGDKEAP
                     HPVLDHDYEIKPCQLEPHEDPARNKAEAEREAQTELQLAS"
     gene            1668..>1998
                     /locus_tag="VGC82_07905"
     CDS             1668..>1998
                     /locus_tag="VGC82_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423231.1"
                     /translation="MIETLQQRVNDDQGLVRRGRHLTTSFLLEVGATPWLISIFEGRI
                     VAVTQGPFVMPSSSFALRASEQEWQKFWSRRPPPGSNDLMALIKRRALKAEGNLQIFM
                     SHLRYFKE"
BASE COUNT          354 a          613 c          612 g          419 t
ORIGIN      
        1 gccgaactgg gtcatgggcg ccgaagccca gtatagctgg ctgcccagct ccaataataa
       61 tggcgtgctg tttccgggtg gcaccctcgt gacctcaaac accgaccagc tcggctcggt
      121 gaccggccgg ctcggctaca cctggggtcc ggcgctgctc tacgccaagg gcggttacgc
      181 ctggcgcgac ggcaacaatc tcggggtgtc ggtggccggc gtgccggcgg ccttcaccac
      241 caacggcagc cacaaggacg gctacaccgt cggcggcggc ctcgaataca tgttcgcccc
      301 gaactggtcg gccaaggccg agtaccagta ttacaatttc ggcaacacca ccttcacgac
      361 aggcccggtc gatatcgccg gccgcagttt ccgcaacgac gaacacactg ccaaggtcgg
      421 cgtgaactac cggtttggtt ggggtggagc cgctgcctcc agatattgat acggcgcttc
      481 gtatcgacac caaaaaggcc ggcttcacgc cggccttttt ttgtctagac gccggagcag
      541 cgatgcttcg tgcgcgttgc cgctgatatt ttgagtgcgg cgaaaataat tttcgcggtg
      601 cacgcaactt ttcgtcatga tgcgctatta ttgatgtcag ccggttgggt tggacatcaa
      661 atcatgcgtg tttttgcgtt agggctgatc ttttccgcag cggcatttcc atgccttgct
      721 cagaccgtcc tgaaatccga accgcttatg cttgctccct atgaggtcgc cttcgtgcag
      781 gattcctcct gcggggccgg caaggtgcgc aaggtaaccg gcgcgatcag gggactgcag
      841 cggcgcaagg cctgtgtcac tctggctgcg gaacaggcat cgctggtctc ggcgacgccg
      901 tagaaccgac ttaccgcctc ggacgtcgtt cccggcgaac gggatcttgc cgagcgttgt
      961 tggttgtctt ctgccgggct gtcctcccgg ccaccatcgc ccttatgacg ccagctgcag
     1021 ttccgtctgc gcttcgcgct cggcttcggc cttgtttcgg gcggggtctt cgtgtggctc
     1081 aagctggcag ggcttgatct cgtagtcgtg atccagcacc ggatgcgggg cctctttgtc
     1141 gccctcggag acgacatcga ccacgaggcc gctcttctgg gcaattttcc agacatcggc
     1201 cgcggtggga taggccttgc tcagcttggc atcgtttgag aacagcgcgt agggcatggt
     1261 cggctccggt aaaaacggtc aacgcagaag cgggggccgg gtttcaggct tggctgccat
     1321 gccgtcattc cactgcttgt ttcgggagca aattggctcg cttcggccca tttttgtgga
     1381 gactctgagt ctttaacgag ccgtaaatcc cggaaaaaag aatccgcgag actcaaccgc
     1441 cagaatcgcc ggatgtttcc ggctatgagc acgagcctcc agacctcctg cgagcgtgtc
     1501 caactcacgc tgaccgtctg cctcttggtc gcctgcgcgg ccaacgtggc cctggtctac
     1561 gcctggctct agcgccggcc gattgacggc cgcctcattc cacggaacat gacggtcaaa
     1621 agcgcccgcc gggcgaagcg gccggcggat caaggatggg gcaggaaatg atcgaaacgc
     1681 tgcagcagcg ggtcaatgac gaccagggcc tggtccggcg cggccggcat ctgacgacca
     1741 gtttccttct ggaagtcggc gccacaccct ggctgatctc catcttcgag ggccgtatcg
     1801 tcgcggtgac gcaagggccg ttcgtgatgc cgtcttcgtc ctttgcactc cgtgcgtccg
     1861 aacaggaatg gcagaaattc tggagcaggc gaccgccgcc gggctcgaat gacctgatgg
     1921 cgctgatcaa acggcgcgcg ctgaaggccg aaggcaatct gcagatattc atgtcccatc
     1981 tgcgctactt caaggaag
//
LOCUS       DASWQN010000481         1945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1233549, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000481 DASWQN010000000
VERSION     DASWQN010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233549"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..809
                     /locus_tag="VGC82_07910"
     CDS             117..809
                     /locus_tag="VGC82_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome (ubi)quinol oxidase subunit III"
                     /protein_id="HEY0423232.1"
                     /translation="MSVSQALPAGHRRDMDPYKLGRGHGGHGGGGHSEATGHGEGGPA
                     SKRVITGYGFWIFLLSDIIMFSAFFAAYGVLGAATAGGPSARDLFEPSRVAIETACLL
                     SSSFTCGLSAVATQVRSQLWTQVSLLITGLLGLAFLILEVQEFAGMIAQNAGPDRSAF
                     LSSFFAVVGCHGLHVTAGLLWLGTMMAQVFVKGFRPNILRRLQCFNLFWHALDIIWVA
                     LFSVVYLIGAGR"
     gene            806..1192
                     /gene="cyoD"
                     /locus_tag="VGC82_07915"
     CDS             806..1192
                     /gene="cyoD"
                     /locus_tag="VGC82_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HEY0423233.1"
                     /translation="MSDTDDGPDPNHGDAAPGEGDVANGIRGYVFGLALAILLTAASF
                     YTAGTQLIWAPGVPVALVVLAIAQIGIHLVFFLHITTAPDNTNNVLALAFGILIVALV
                     IGGSLWIMANLNQNMVPMEQMMQMQR"
     gene            complement(1226..1654)
                     /locus_tag="VGC82_07920"
     CDS             complement(1226..1654)
                     /locus_tag="VGC82_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0423234.1"
                     /translation="MADAILLDTHIALWLDSANDRLRKSTRDLIDACWHDGGTILISA
                     VTVWEIAQLVDSGRIILDRSVKAWLERFTDRPGVEAVPLNARAAAGAYRLDHLDHRDL
                     ADGLLVATAIELSCPLVTYDQRIARFGADHGTRYGFATRD"
     gene            complement(1726..>1945)
                     /locus_tag="VGC82_07925"
     CDS             complement(1726..>1945)
                     /locus_tag="VGC82_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423235.1"
                     /translation="KGTIIIPIMIEVPMPSVVTINRPDVVALIQSAADRLTGGNKTEA
                     VAMAVRSLLERNARTGSLFGAHPGSVTG"
BASE COUNT          327 a          604 c          628 g          386 t
ORIGIN      
        1 tggcgggatg tcgatgaata ccgaatcccg gcggaggaag tcgctgcgat cgatcgcgca
       61 cggcggcgtg cccgtgagga gtggcttttg gccaacgccc gcgtgggcga gccggtatga
      121 gcgtttccca ggcgctaccg gcggggcatc gccgggacat ggacccctac aagctgggcc
      181 gcggccacgg cggccacggt ggcggggggc acagcgaagc gaccggtcat ggcgagggcg
      241 gtcccgcttc caagcgcgtg atcaccggct acggcttctg gatcttcctg ctcagcgaca
      301 tcatcatgtt ctccgcgttc ttcgcggcgt acggggtgtt gggggcggcg acggccggcg
      361 gtccatcggc ccgcgacctg ttcgagccat cgcgggtcgc gatcgaaacg gcatgccttc
      421 tctcgtccag cttcacatgc ggtctttccg cggtggccac ccaggtccgt agccagcttt
      481 ggacccaggt ttcgttgctg ataaccggtc tcctgggcct cgccttcctg atactggaag
      541 tgcaggaatt cgccgggatg atcgcccaga acgcgggtcc cgaccgcagc gctttcctgt
      601 cgtcgttttt cgcggtcgtc ggatgtcacg ggcttcacgt tacggctggg ttgctgtggc
      661 tgggcaccat gatggcgcag gtattcgtca agggcttccg ccccaacatt ctgcgccggc
      721 tgcagtgctt caacctgttc tggcacgccc tggatatcat atgggtcgcg ctgttctcgg
      781 tcgtttatct gataggagcc ggaagatgag cgacactgac gacggcccgg atccgaatca
      841 cggagacgcg gcgcccggtg agggcgacgt cgcaaacggc atccgcggct atgttttcgg
      901 tctcgcactg gcgatcctgc tgaccgcggc ctcgttctac acggctggca cgcaactgat
      961 ctgggcgccg ggggttcccg tcgcgctggt cgtgctggcg atcgcgcaga tcggcataca
     1021 cttggtgttc tttcttcaca tcacgaccgc gcccgacaac accaacaacg tgctcgcttt
     1081 ggcctttggc atcctgatcg tagcgctggt catcggtggt tcgctgtgga tcatggccaa
     1141 cctcaaccag aacatggttc ccatggaaca gatgatgcag atgcaaaggt gaggcttatg
     1201 cggacgttgg cccgtgggtc accgatcagt ccctggtcgc gaacccatac cgggtgccat
     1261 ggtcggcgcc gaaacgcgcg attcgctggt cataggtaac gagcgggcaa gacaactcga
     1321 tcgccgtggc gaccagcagc ccatcggcga ggtccctgtg gtcgagatgg tccagccgat
     1381 acgcgcccgc cgcggcccgg gcattcagcg gcaccgcctc taccccgggc cgatccgtga
     1441 agcgttccag ccatgctttg accgaacggt cgagaatgat ccgtcctgaa tcgacaagct
     1501 gcgcgatctc ccagactgta accgcgctga tcaggatcgt cccgccgtcg tgccaacagg
     1561 cgtcgatcag gtcgcgggtt gacttgcgaa ggcggtcgtt agcgctgtcg agccacaagg
     1621 cgatgtgggt gtcgagcagg atggcatcag ccacgatcga gttcgtggcc ggtttcgccg
     1681 tccatcgggt cctgcatgac gggcgcgatc aaatccacac cttccttaac ccgtcacgga
     1741 gccaggatgc gcaccgaaca ggctaccagt gcgggcattc cgctccagca gggaccgcac
     1801 ggccatggcg acggcttcgg tcttgtttcc gccggtcagc cgatcggctg ccgactggat
     1861 caaagcgaca acatcagggc ggttgatggt aacgacgctc ggcatgggga cctcgatcat
     1921 tataggtata atgatagtcc ctttg
//
LOCUS       DASWQN010000482         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1101249, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000482 DASWQN010000000
VERSION     DASWQN010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101249"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..810
                     /locus_tag="VGC82_07930"
     CDS             97..810
                     /locus_tag="VGC82_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEY0423236.1"
                     /translation="MTQLLFLDHPGLANTEATVVASGPDGIVLDRTVFYARGGGQPGD
                     SGVLRWDGGETQISEAVKGPAEAILHLPIPEAPLPTVGTVVDAAVDWQRRFAHMRMHT
                     AMHLLCSLIKGAAVTGGAVGADKSRLDFDLPNPPPKDELEAGLNALIAADHPVRIKWV
                     DESVLDTDPGLVRTMSVKPPRGTGRLRLIRIGESETPVDLQPCGGTHVSRTREIGPMR
                     IQKIENKGKQNRRIVIVAA"
     gene            917..1144
                     /locus_tag="VGC82_07935"
     CDS             917..1144
                     /locus_tag="VGC82_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0423237.1"
                     /translation="MSIADVLHGKGHQVVKVRTTDTVQTAVRKLSDERIGAVVVEDQW
                     MHHAGIFSERDFVNAVAEHGPQALTFRWKSS"
     gene            1141..1350
                     /locus_tag="VGC82_07940"
     CDS             1141..1350
                     /locus_tag="VGC82_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0423238.1"
                     /translation="MSAPIVTCRPNDRVETAMAAMTMAKIRHLPVIEGGQLIGIVSIG
                     DLVKHRLDEKALEANVLLDLARLHS"
BASE COUNT          390 a          622 c          608 g          343 t
ORIGIN      
        1 agcattcgcg ggctgtttca ccaactcaat aacagtgatc ggcctgttct ggctggcgag
       61 ccggcgcgac tggctgcgac agaaatggat gacccaatga cccagcttct tttcctcgac
      121 cacccggggc tcgccaacac cgaagcgacc gtggttgcat ccggaccgga tgggatcgtg
      181 ctcgaccgca ccgtctttta cgcgcgcggc gggggccagc caggcgacag cggcgtgctg
      241 cgctgggatg gcggcgagac ccagatttcc gaagccgtga aaggtccggc tgaagcgatc
      301 ctgcatctcc cgattccgga ggcgccgctg ccgacggtcg gaacggtcgt ggacgccgcc
      361 gtcgactggc agaggcgctt cgcccacatg cggatgcaca ctgcaatgca tctgttgtgc
      421 agcctgatca agggggccgc ggtcaccggc ggggcggtcg gcgcggacaa gtcccggctt
      481 gatttcgacc tgcccaatcc accgccaaag gatgaactcg aggccggcct gaacgccctg
      541 atagccgccg atcatccggt acggatcaaa tgggtggacg aatcggtcct ggacaccgac
      601 cctggcctgg ttcgcacgat gtcggtcaag ccgccgcgcg gcaccgggcg gctgcgcctg
      661 attcggatcg gcgagagcga aacgccggtc gatctgcaac cgtgcggcgg gacacacgtg
      721 tcgcgaaccc gcgagatcgg gccgatgcgt attcagaaaa tcgagaacaa aggcaaacaa
      781 aaccgccgca tcgtcatcgt ggcggcctga ttcgcaccga tgctgtctgt tacctgaaca
      841 attggccatc ttcttgggat cgtgccgccg cctgcccatc tgaatactgg gttgtttcag
      901 aaaaggagcg gttcccatgt ctatcgctga cgtgttgcat ggcaaaggcc atcaggtcgt
      961 caaggttcga accaccgata cggtgcagac ggcggtgcgc aaactgtcag atgaacggat
     1021 tggcgccgtc gtggtcgaag atcagtggat gcaccatgcc ggcatcttct cggagcgcga
     1081 tttcgtcaac gccgtcgcgg aacatggccc tcaggcactg acctttcggt ggaaaagctc
     1141 atgagcgcgc cgatcgtgac ctgccgtccg aacgaccgcg tggagaccgc catggcggcg
     1201 atgacgatgg cgaagatcag gcacctgccg gtcatcgaag gcggacagtt gatcggtatc
     1261 gtcagcatcg gcgacctggt gaagcaccgg ctcgatgaaa aagcgctcga agccaatgtt
     1321 ttactggatt tggcacggtt gcatagctga cccggatcgg cgggctcacg ttgacggtta
     1381 aacccttcga tttgggctgc gcagatttgg tccgcgcggg gcaaacacgg ccgcgctgag
     1441 tgaaggtcta acatcaggtc gccatcacgc tccggcagtt ccagccacgc tccatggcgc
     1501 aaccaaggtt cgaagggcgt tcaaagatcg ggcgccgtcc ggtgcatcgg ctccgcccat
     1561 gccgatccaa cggttgcttc caacgcagcg gacgcagagg acgctgacat cggagcctat
     1621 ctgagatcgc tgagaagttg cccgtgcgcc tcggcgctat ggcacgggcg catcctccac
     1681 ccaagccgcg cagccgcccc aaaccaaggt cccgatcgtc aacagcaagg caggatttaa
     1741 ccatccgtgc cctccataat ctgcatgccc tctgtgttga gagccttggc caaccgccgc
     1801 acggacctcg aaagcccccg aaaacgaccc cggccgcata atccgcctat cccgaagtcc
     1861 gcaggctcga cgtcattgga acgcgataca tggtgccttg ccgacccctg ttcctgagtg
     1921 gccgaacccc ggggccggcg cgccaccgcg tggcaagtag gcc
//
LOCUS       DASWQN010000483         5481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_969426, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000483 DASWQN010000000
VERSION     DASWQN010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969426"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..564
                     /locus_tag="VGC82_07945"
     CDS             49..564
                     /locus_tag="VGC82_07945"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY0423239.1"
                     /translation="MDLKDHIRGIPDFPKPGILFYDISTLLRDADAWQVAMGRMARAV
                     RAYMPDLVAGVESRGFLVAAPLALKLGCGFIMIRKRGKLPGETVGLNYALEYGEDRVE
                     IQADAVHPGQRVVVVDDLLATGGTMAAGIALLRQVGAVVPAAATLIELNFLNGRSRLD
                     VPCESLVSYDD"
     gene            complement(608..1141)
                     /locus_tag="VGC82_07950"
     CDS             complement(608..1141)
                     /locus_tag="VGC82_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0423240.1"
                     /translation="MAERILGAANRLFYQKGIRAVGVDAVAAEAGISKRSLYDTFPSK
                     DALVAAYLRQRIVPLPSSDMPPSAQVLALFDQLHARFASGDFRGCPFVNAVTELAEDC
                     ETARAIASDYKEERRQHIASLLSLAGALHPDALASQVALLIEGAIASMLVRQDPEVAV
                     QAREAAAVLMRAGGATT"
     gene            1288..2121
                     /locus_tag="VGC82_07955"
     CDS             1288..2121
                     /locus_tag="VGC82_07955"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEY0423241.1"
                     /translation="MDVVSSLPKVTGEVVYLAKTQEKPQTYTYDPPDGVAKTNIVNDP
                     HTVPIFDMRPIADGLNLDVQGFALVDAPTSVGDFYDEEQLRTTYYREAEDLVKQATGA
                     SRVVVFDHTIRKRTHGVEDRTPGIPRQPVSRIHGDYTELSGPQRVRDLMGAEADDLLK
                     RRFAIVNVWRPIRGPLFDAPLAVCDAGSLADGDLVGQDLIYRDRTGEIYGLTFNPEHR
                     WYYAPAMQADEALLLKCFDSAKDGRARFMPHTSFADPNAPSDMLPRESIELRTLVFFD
                     A"
     gene            2184..3965
                     /locus_tag="VGC82_07960"
     CDS             2184..3965
                     /locus_tag="VGC82_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY0423242.1"
                     /translation="MTLHQIEALAIIAGMLVLFLSDRLRYDMVGASALSAAALLGVVP
                     AHKAFSGFSSPVVIIIASVLVMSRAVEVSGIIDRAMRRILQGVRSTTMQVGLLTGAVT
                     FLSAVVKNVGALGIFMPVAIQAAERRNRPVSRYLMPLAFGSLIGGTITQIGTSPNILI
                     SQVRQEVTGHPYGMWDYASIGLPLSVVAVLFLSVGWRLLPANRRGQPSSGKRFKVEDY
                     TTETLLPANSDLVGRTVSDLETASEHQFVITAIIREQTHRYVPRGHWVLYAEDILILQ
                     GDPAALQPLIDQGQLSLLNGEEVGGLKPEGKDDDLETVEAVVSPASALIGQTVQTLQM
                     RSRYEVNVLAAGGAGRTPHRLRQRAFAAGDIVVLQGRTSRLNDVLRQLGLLPLAERSL
                     AIGQKRARYLPLVILAVALLAIGLRLVEVEVGFFVAATVVLLLRLISVREAYEAIDWP
                     VIVMLGCLIPVGEALKDTGAAALMADGLTVLASHLSGTLAVALILVVSMLVTPFLHHA
                     AAVLVMGPVAAAVAKNLGYAVDPFLMAVAFGAACDFLTPIGHQNSTLVMRPGGYRFGD
                     YWRLGLPLSVLVAVAGTWLIVWSWPLG"
     gene            complement(3990..4739)
                     /locus_tag="VGC82_07965"
     CDS             complement(3990..4739)
                     /locus_tag="VGC82_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0423243.1"
                     /translation="MQITSGDRVRMERAHVLAWPALRTAIVDGWLWRSSGGGSQRANS
                     VSTIEFTGHDLEASIDRVEARYRAINQPPRFQTFDHTCPAGLTDALRARGYTEGQATL
                     TMFKPSSPLAAPSQVESRDYPWDEWCDIYLAEITESRRMVNRRILSAIPKPCQYFASR
                     RDGRIISTALCVVGFGCAVVECVASRSCSRRQGGAASVMAGLLAWAARQDADLVGLQV
                     VADNTPAVRLYEALGFVPHATNKFWLHDPVV"
     gene            4829..>5481
                     /locus_tag="VGC82_07970"
     CDS             4829..>5481
                     /locus_tag="VGC82_07970"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0423244.1"
                     /translation="MPAFDLVIRGGTVVDGTGSAPREADVGLIGNKIAAVGEVAGRGA
                     EEIDAKGKLVTPGFVDIHTHYDAQAVWDSHMTPSSLHGVTTAVMGNCGVGFAPCKPAD
                     RERLIELMEGVEDIPGAVMHEGLKWEWESFSEYLDALDRRNRDIDICALLPHAAVRVF
                     VMGDRAIQLENANQGDIAQMRQIAKEAMQAGAFGFSTSRSLSHKTLRGDPTPTLRAQ"
BASE COUNT          937 a         1644 c         1883 g         1017 t
ORIGIN      
        1 cgcgcatggc actagggtgc gcggcgccaa aaccgaggac ctacgtcgat ggacctgaaa
       61 gaccacattc gcggcattcc ggattttccg aagcccggca tcctgttcta cgacatttcc
      121 acgctgctgc gggatgccga cgcgtggcag gtcgcgatgg gccggatggc acgcgcggta
      181 cgggcttata tgccggatct ggtcgcgggg gtggaaagcc gcggcttcct ggtcgccgca
      241 ccgctggcgc tgaagctggg ctgtggcttc atcatgatcc gtaagcgtgg caagctgccg
      301 ggggagacgg tgggattgaa ttatgccctg gaatacggcg aggatcgcgt cgagattcaa
      361 gccgacgcgg tgcatccggg ccagcgcgtg gtcgtggttg acgatctgct tgccactggc
      421 gggaccatgg cggccgggat cgcgctgctg cgccaggttg gcgcggtcgt gccggccgcg
      481 gcgacgttga tcgagctgaa tttcctgaac gggcgctcgc gtctggacgt gccgtgcgag
      541 tcgcttgttt cgtatgacga ttgaaaatcc gacgcaggcc agccgcgacg tgagacggag
      601 ggcgccttta tgtggtcgcg ccaccggctc gcatcagcac cgctgccgcc tcgcgggcct
      661 gaacggcgac ctcgggatcc tgccgtacca gcatcgacgc gatggcgccc tcgatcagca
      721 gggcgacctg actggcaagc gcatccgggt gtagcgcgcc ggccagggac agcagcgaag
      781 caatatgctg gcgcctctct tccttgtagt ccgaagcgat ggcacgcgcc gtttcgcaat
      841 cctcggccag ttccgtcacg gcgttgacga aggggcagcc gcggaagtcg ccgctggcga
      901 agcgggcatg cagttggtcg aacagtgcca ggacctgagc ggacggaggc atgtccgacg
      961 acggaagcgg cacgatccgc tgccgcagat aggccgcgac aagggcatcc ttcgacggga
     1021 acgtgtcgta aagagaccgc ttgctgatgc cggcctccgc ggccacggcg tccactccga
     1081 cggcgcggat gcccttctgg tagaacagcc ggttggccgc ccccagtatc cgttcggcca
     1141 ttgccggtcg ttccgccacg ttgctcagcc tttcgtgccc aaatgatttc acttgccaag
     1201 agccggggcg caccccatct gcgctccaca ccgatcggtg tgtttgatat cggcattgtc
     1261 agccgatcgg caaggaggac aggcatcgtg gacgttgttt cttcgcttcc caaggtcacc
     1321 ggggaagtcg tgtacctggc gaagacccag gaaaagccgc aaacctacac ctacgatccg
     1381 ccggatggcg tggcgaagac caacatcgtc aacgacccgc ataccgtgcc gatcttcgac
     1441 atgcggccga ttgcggacgg cttgaatctg gatgtgcaag gcttcgcact ggtcgatgcg
     1501 ccaacgtcgg tcggcgactt ctacgacgag gagcagttgc gcacgaccta ttaccgggaa
     1561 gccgaggacc tggtgaaaca ggccaccggg gcaagccggg tggtggtgtt cgaccatacc
     1621 atccgcaagc ggacacatgg ggtcgaggac cggacgccgg gtatcccgcg gcagcccgtt
     1681 tctcgcatcc acggcgacta taccgagctt tctggcccgc aacgtgtgcg cgacctgatg
     1741 ggtgcggaag ccgacgatct gctgaagcgc aggttcgcca tcgtgaacgt ttggcgcccg
     1801 attcgtgggc cgctgttcga tgcaccgctg gcggtctgcg acgccgggtc attggcggat
     1861 ggcgatctgg tgggacagga cctgatctat cgcgatcgca ccggggagat ttacggcctg
     1921 accttcaacc ccgagcaccg gtggtattat gcgccggcga tgcaggcgga cgaggcgctg
     1981 ctgctgaagt gcttcgactc cgcgaaggac ggccgcgctc ggtttatgcc gcacacgtcg
     2041 tttgccgacc cgaacgcgcc gagcgatatg ctgccgcgcg aaagcatcga gcttcggacg
     2101 ctggttttct tcgatgcatg atcggcttgt tgtttcccgg gaggctccca accttcgacc
     2161 gtgaacggtc ggggtgagaa cgcttgacgc tgcatcagat cgaggccctg gcgatcatcg
     2221 ccggaatgtt ggtcctgttt ctgtcggacc gattgcgtta cgatatggtt ggcgcttccg
     2281 cgttgagtgc cgccgcgctg ctgggggtcg tgccggcgca caaggccttc agcggcttct
     2341 ccagccccgt cgtcatcatc atcgcctcgg tcctggtcat gagccgagcg gtggaggtgt
     2401 ccgggataat agaccgcgcg atgcggcgga tattgcaagg cgtgcgctcg accacgatgc
     2461 aggtcggcct gctgacgggg gcggttacat ttctgtccgc cgtcgtgaaa aacgttggtg
     2521 cgctgggtat cttcatgccg gtggcgatcc aggccgcgga acgcaggaat cggccggtgt
     2581 cgcgctattt gatgccgctg gcattcgggt cgttgatcgg cggcacgatc acgcagattg
     2641 gcacctcgcc caatatcctg atctcgcagg tgcgccagga ggtgaccgga catccgtacg
     2701 gcatgtggga ctatgcctcg atcgggctgc cgctgtcggt ggtggccgtg ctgttcctga
     2761 gcgttggctg gcggctgctg ccggcaaacc gccgcgggca gccgtccagc ggaaagcgct
     2821 tcaaggtcga ggactacacc accgaaacgc tgctgccggc gaactcggat ctggttgggc
     2881 ggacggtaag cgacctcgag accgcaagcg aacatcagtt cgtaattacg gcgatcatcc
     2941 gcgaacagac gcatcgttat gtgccgcgcg gccactgggt gctgtacgcc gaagatatcc
     3001 tgatcctgca gggcgatccg gccgccttgc agccgcttat cgatcaggga caattgtcgc
     3061 tactgaacgg cgaggaagtc ggaggcctga aacccgaagg caaggacgac gacctggaga
     3121 cggtcgaggc ggtggtgtct ccggcgtcgg cgttgatcgg tcagaccgtg caaacgctgc
     3181 agatgcggtc gcgttacgag gttaacgtgc ttgctgccgg tggcgccggc aggaccccgc
     3241 acaggctccg gcagcgagcc ttcgcggcgg gcgacatcgt ggtgttgcag gggcgcacca
     3301 gccggttgaa cgacgtgctg cggcaactgg gtctgctgcc gctggcggag cgcagcctgg
     3361 cgatcggaca aaagcgcgcc cgttatcttc cgctggtgat cctggcggtt gcgctgctgg
     3421 cgatcgggtt gcgcctggtg gaggtcgagg tcgggttctt tgtcgcggca acggttgtgc
     3481 tgctgctgcg gctgatctcg gtgcgggagg cttacgaggc catcgactgg ccggtgatcg
     3541 tcatgctggg ctgtctgatc ccggtggggg aggcgctgaa ggacaccggc gcggcggcac
     3601 tgatggcgga cggattgacg gtgctggcat cgcatctgtc cgggaccttg gcggtggcat
     3661 tgatcctggt ggtgtcgatg ctggtgacgc cgttcctgca ccatgcggcg gcggtcctgg
     3721 tgatgggtcc ggtcgcggcg gcggtggcga agaacctcgg gtatgccgtc gatccgttcc
     3781 tgatggcggt ggcgttcggc gcggcctgcg atttcctgac gccgatcggt caccagaaca
     3841 gtacgctggt gatgcggccg ggtgggtatc ggttcggcga ttactggcgt ctggggttgc
     3901 cgttgtcggt gctggttgcc gtagccggga cgtggctgat cgtctggtcg tggccgctgg
     3961 ggtaagcatc cgagcgcaaa ttcgaccgac tatactacag ggtcgtgcag ccagaatttg
     4021 ttggtggcgt gcggaacgaa gcccagggct tcgtaaagcc ggaccgccgg cgtattgtcg
     4081 gcaaccacct gcaagccgac aaggtcggcg tcttgccgag cggcccaggc aagcagtccg
     4141 gccatgacgg aagcggcgcc gccttgtcgg cggctgcagg atcgtgacgc cacgcactcg
     4201 acgacggcgc acccgaaacc gacgacacaa agagcagtcg aaatgatccg gccatcacgg
     4261 cggctggcaa agtattggca gggcttcggg atcgccgaca agatccggcg attcaccatg
     4321 cggcggcttt cggtgatttc agccaggtag atgtcgcacc attcgtccca gggatagtcc
     4381 cgggattcga cctgcgatgg agccgcgagc ggactcgatg gcttgaacat cgtcaacgtt
     4441 gcctggcctt cggtgtaccc gcgtgcacgc agcgcatcgg tcaaaccagc ggggcaagtg
     4501 tgatcgaacg tttggaaccg cgggggttgg ttgatggcgc ggtatcgtgc ctcgaccctg
     4561 tcgatcgacg cctcgaggtc atggccggta aattcgatcg tcgagaccga gttcgcccgt
     4621 tgcgatccgc cgcccgacga ccgccaaagc catccgtcaa cgatggcggt gcgcagtgcc
     4681 ggccacgcca ggacatgggc acgctccatc cgcacgcgat cgccgctggt gatctgcatc
     4741 cgataggctc cgtaacttgc caacctcaaa cttgccagct tgcgtgggcg ggatcaggat
     4801 cggtctaaat catacaggag aaagtcctat gccagcattc gatctcgtca tccgcggcgg
     4861 tacagtcgtc gatggcaccg gcagcgcccc gcgggaggcc gatgtcggcc ttatcggcaa
     4921 caagatcgcc gctgtcggtg aggtggcggg ccgcggcgcg gaagagatcg atgccaaggg
     4981 caagctggta actccgggat ttgtcgatat ccacacgcat tatgacgctc aggcggtgtg
     5041 ggacagccac atgacgccgt cctcgctgca tggcgtcacc acggccgtca tgggcaactg
     5101 cggcgtcggc ttcgccccct gcaagccggc cgaccgcgag cggctgatcg agctgatgga
     5161 aggcgtcgag gacattcccg gcgcggtgat gcacgagggc ctgaaatggg agtgggaatc
     5221 gttcagcgaa tacctcgacg ccctggaccg ccgcaaccgt gacatcgaca tctgcgcgct
     5281 gctgccgcat gccgccgtgc gcgtcttcgt catgggcgac cgggcgatcc aattggagaa
     5341 cgccaaccag ggcgacatcg cacagatgcg tcagatcgcg aaagaggcga tgcaggctgg
     5401 cgctttcggg ttttcgacct cgcgcagcct gtcgcacaag acgctgcgcg gcgatccgac
     5461 cccaacgctg cgcgcccagg a
//
LOCUS       DASWQN010000484         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1365961, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000484 DASWQN010000000
VERSION     DASWQN010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365961"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..>1535)
                     /locus_tag="VGC82_07975"
     CDS             complement(163..>1535)
                     /locus_tag="VGC82_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0423245.1"
                     /translation="AGSEQAHFYGSILLAPGGRHGCTSGCWHPDAALPLDLRLWISEI
                     IAALPGRCAVTVLELLTGTLLTGYGSISQAILAVPCRRHWTSYYWMVRKGQFGWKAMV
                     HALCRIVRREFPSERCVVVGDDTLIPRVSAAAPGAAIQYDHARKPNRPDYILAQTVVS
                     LSAVIGNSLGSWSVPLISNLAVRTGNHGKLETMRAMLEEVSGTLGAITLLLDAWYMKG
                     YLILDALKRDAIVIGQVRRDTALYDPPPSRTGKPGRPRKYGDRITKEKLKTRAQTHTE
                     LSIYGGQRTVRYRTAVCRARFLGGRLVRAVWVSMLVDGQWKAERLLPSTDWSHSAEYV
                     ISTHSLRWTVEPMFAAMKWGEGMIGMWMRSVETFHRWLNIVQIGRALIQMLAVKADAA
                     TQALAQVAAWRKERYLTAGMVKTGLVRAFWNIAPAEAWHRRGRKFDPSRRVPIRTRVV
                     HTASAA"
BASE COUNT          283 a          493 c          487 g          272 t
ORIGIN      
        1 gctcgccttc gcccacatac ccacaggctc aacaacaagc agaaactgat tcatatcggg
       61 aaggaagggt gactagacat tcgagcttcg cggcatagcg gttcggttcg gcgggagccg
      121 gatgggccgc gagcagctga atttgatgcc ggaaaggctg gatcaggcgg cactggcggt
      181 gtgaaccacc ctggtcctta tcggaacgcg tcgggacggg tcgaatttcc gccctctgcg
      241 gtgccacgct tctgccggcg caatattcca gaaggctcgg acgaggccgg tcttgaccat
      301 gccggcggtg agataccgct ccttgcgcca ggctgcgacc tgagccagcg cctgggtcgc
      361 ggcatcggcc ttgacggcca gcatctggat cagcgcccgc ccgatctgga cgatgttcag
      421 ccatcgatgg aaggtttcga ccgaccgcat ccacatgccg atcattccct cgccccattt
      481 catggcggca aacatcggtt caaccgtcca gcgcaaagag tgggtggaga tcacgtattc
      541 cgctgaatga ctccaatcag tcgacggcag aagccgttct gctttccatt ggccatccac
      601 caacatcgat acccaaacgg ctcggacaag acggccaccg aggaagcggg ccctgcagac
      661 agcggtgcga tagcgaacgg tgcgctggcc gccgtagatc gacagctccg tgtgcgtttg
      721 cgcccgcgtc ttgagcttct ccttggtgat gcggtcgccg tacttgcggg gccgtcctgg
      781 ttttcccgtc cgcgatggcg gcggatcgta cagggcggtg tcgcgtcgga cctggccgat
      841 gacgatggcg tcgcgcttga gggcatcgag gatcagatac cccttcatgt accaggcgtc
      901 gagcagcagc gtgatcgcgc ccagggtccc gctcacctcc tccagcatgg cccgcatcgt
      961 ttcgagcttg ccgtggttgc cggtgcgcac cgccaggttc gatatcagcg gcacgctcca
     1021 ggatcccagg gaattgccga tcaccgccga caggctcaca accgtctggg ccaggatata
     1081 gtccggccga ttgggtttcc gggcatgatc gtactggatt gcggcgcccg gcgccgccgc
     1141 ggacacgcgc gggatcaggg tatcgtcgcc gaccacgacg cagcgctcgg atgggaactc
     1201 ccgcctcacg atgcggcaca aggcatggac catcgctttc cagccgaact gccccttgcg
     1261 caccatccaa tagtagctcg tccagtgccg ccggcatgga accgccagaa tcgcttggct
     1321 gatcgagcca taaccggtca gcagggtgcc ggtcagcagt tccaggaccg tgacggcaca
     1381 gcgccccggg agcgcggcga tgatctcgga gatccagagg cggagatcca gaggcagagc
     1441 agcgtcggga tgccaacagc cgctcgtgca accatgccgc cctccgggtg cgaggagtat
     1501 gctcccgtag aagtgggcct gttcgctccc ggcag
//
LOCUS       DASWQN010000485         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2025472, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000485 DASWQN010000000
VERSION     DASWQN010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025472"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VGC82_07980"
     CDS             <1..357
                     /locus_tag="VGC82_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY0423246.1"
                     /translation="VNLSAMTGVGVDRLLPTVRAAHEIWNKRIPTGELNRWFEYALET
                     HQAPLVSGRRLKLRYATQAKARPPTFVVFGTRAEQTPEDYSRYLVNSLRDTFKLPGTP
                     IRLQFRGTKNPFVNDE"
     gene            798..1661
                     /locus_tag="VGC82_07985"
     CDS             798..1661
                     /locus_tag="VGC82_07985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0423247.1"
                     /translation="MTDQAMTDQAMTDQDWRALNKANWNERVPIHLAAPNDYDQTPLR
                     NGTARLEPIASAAIGPVAGLNILHLQCHFGMDSLTIAQQGASVVGVDFSEPAIVAARA
                     LAVELGLHDRSRFIEADLYGAPSALPEPGGFDRVFVTWGALNWLPDIAGWARVVAYFL
                     KPGGWLALAEAHPAAYVFDDAMRTPDGMPGWYVPYLGRTAIMEDRPEDYADPSAVLTN
                     SRFVEWLHPLSDIVMGLLGAGLRLEFLHEHDTVRWKMFDCLVPDGRGGFRWPDKPWLP
                     LSYSLRASKPA"
     gene            complement(1688..>2060)
                     /locus_tag="VGC82_07990"
     CDS             complement(1688..>2060)
                     /locus_tag="VGC82_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0423248.1"
                     /translation="SKARSVMHTGTELPVVPVSAVLSEAIVEMTSKRFGITGVVDEAG
                     YLVGVLTDGDLRRAFKNGFTDGPVAKVMGRRPLSVGPEALAVEVLAHMNQSRITCIFV
                     VQDQRPIGLIHVHDLLRVGIV"
BASE COUNT          386 a          650 c          635 g          389 t
ORIGIN      
        1 gtgaatcttt ccgcgatgac cggcgttggc gtcgatcggc tgttgccgac ggtgcgtgcg
       61 gctcatgaga tctggaacaa gcgcatcccg acgggagagt tgaatcgctg gttcgaatat
      121 gccctggaaa cgcaccaggc accgctggtc agcggccgtc gcttgaagct gcgctacgcg
      181 acccaggcca aggcccgccc cccgactttc gtggtcttcg gcacacgggc ggagcagacg
      241 ccggaggatt attcgcgcta tctggtgaac agcctgcgcg acacgttcaa gctgcccggc
      301 acgccgatcc ggctgcagtt ccgtgggaca aagaatccgt tcgtgaacga cgagtagcca
      361 ggccctgatc ttgcacttcc cttaccgggc aaggaaggcg gcacatcaac ggcttgggcg
      421 ggctcaacaa atcttcgaat ggcgtcgtca tcggcttggt gcagctgaag ctgcccgcga
      481 tcgcaacccg cgcccatatc gcggcacaga cggccaggat ggtcgccatg gtttgcaagg
      541 ccaggcgcgg cataggcacg atcgaccttg tgatgttccc cgaatacgca cttcgcggcg
      601 tgtcgatgga cacccgcgat gaaatcctgt gcgagatcaa tggtccgagg tggcggcatt
      661 caaggcagca tgcctcaaaa ataaggtctg gggctgtttc tccatcatgg agcgcaatcc
      721 cgttggccga agccgcggaa caaccttcac gcggctgcct gcttggtcat agggtccgta
      781 aaacggaggt agcatccatg accgatcagg ccatgaccga tcaagccatg accgatcaag
      841 attggcgcgc gcttaacaag gcgaactgga acgagcgggt gccgatccac ctcgccgcgc
      901 cgaatgacta cgaccagaca cctttgcgca acggcacggc tcgcctcgaa ccgatcgcga
      961 gcgccgcaat tggtcctgtt gcgggattga atatcctcca cctgcaatgc catttcggga
     1021 tggacagcct gaccatcgca caacagggag cctcggtcgt tggagtggat ttctccgaac
     1081 ccgcgatcgt ggccgcgcgt gccctggctg tggaactggg cttgcacgac cggtcccgct
     1141 ttatcgaggc ggatctctac ggggcgccat ccgcgttgcc tgaacccggt ggtttcgacc
     1201 gcgtcttcgt cacttggggg gcgttgaact ggctgcccga tatcgcaggc tgggcgcgcg
     1261 tggtggcgta tttcctgaag ccgggcggtt ggctcgcgtt ggccgaagca catcccgctg
     1321 cttacgtttt cgacgatgcc atgcgaacgc ccgacgggat gccaggatgg tatgtgccct
     1381 atcttggccg aaccgcgatc atggaagacc ggccggagga ttacgccgat cccagcgccg
     1441 tgctgaccaa cagccgcttc gtggagtggc tgcatccgct tagcgatatc gtcatgggcc
     1501 tgctgggcgc cgggctgcgc cttgagttcc tgcatgagca tgacaccgtg cgttggaaga
     1561 tgttcgactg cctggttccc gacggccgcg gcgggttccg ctggcctgac aaaccctggt
     1621 tgccgctttc atactcgctg cgcgcgagca aacccgcctg acgtccgatc aatgctcgta
     1681 agtggcgcta cacgatcccc actcgcagca agtcgtggac atgaataagc ccgatcggcc
     1741 tttgatcctg aaccacgaag atgcaggtga tgcgcgactg gttcatgtgc gccaaaacct
     1801 cgacagccag ggcttcgggg ccaacgctga gtggccgccg gcccatcacc ttggcgaccg
     1861 gcccgtcggt gaagccgttt ttgaacgcgc ggcgcagatc gccgtcggtc agcaccccaa
     1921 caaggtagcc cgcctcgtcc accaccccgg taatgccgaa gcgcttcgag gtcatctcga
     1981 cgatcgcctc ggacagcacc gcgctgaccg gcacaaccgg taactcggtc ccggtatgca
     2041 ttacgctccg agccttcgag
//
LOCUS       DASWQN010000486         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_705380, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000486 DASWQN010000000
VERSION     DASWQN010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705380"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VGC82_07995"
     CDS             complement(<1..280)
                     /locus_tag="VGC82_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0423249.1"
                     /translation="MTEGFTVTQGFATYPSLRDRVVLVTGGASGIGAEEVTQFARQGA
                     KVAFLDVSDDAARQLSDTLGGESLPRPHYVHCDLKDIPAVQAAIAEIVG"
     gene            complement(277..765)
                     /locus_tag="VGC82_08000"
     CDS             complement(277..765)
                     /locus_tag="VGC82_08000"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEY0423250.1"
                     /translation="MGVSGSGKTTVAAGLARQKGWLLLEGDSFHPPANIAKMKVGIPL
                     TDDDRWPWLQAIAAREDELIAAGQSAVVACSALKRAYRDILMGGRPDTVLVYLRGSQA
                     LIAERMKARQNHFMPPALLDSQFATLEEPAPDEHPIVVDIGGSAEAAINKAISQLKER
                     LP"
     gene            complement(780..938)
                     /locus_tag="VGC82_08005"
                     /pseudo
     CDS             complement(780..938)
                     /locus_tag="VGC82_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010501124.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
     gene            complement(1185..>1916)
                     /gene="surE"
                     /locus_tag="VGC82_08010"
     CDS             complement(1185..>1916)
                     /gene="surE"
                     /locus_tag="VGC82_08010"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY0423251.1"
                     /translation="APGMQVLEEIAALIAREVWVVAPEHDQSGQSHAISLHHALRISE
                     RGNRRFGVTGTPGDCAAMGLCHIMKDCPPQLVLSGVNRGLNLGLETVFSGTVGGAMTA
                     MMLGVPALALSQAFTNRDAVPWDTARTLGAETIRSLLAVGWSKTAILNINFPPIPAAE
                     AGPVTLARQGEGAVAGMHVETRVDPRGMSYHWLNFKRGDLQQGPESDYSALRARKIVV
                     TPLRYDRTDDDAYTELAAHLPRFGV"
BASE COUNT          338 a          629 c          587 g          362 t
ORIGIN      
        1 gtccgacgat ttccgcgatc gcggcctgga cggcgggaat atctttcagg tcgcaatgca
       61 cgtaatgcgg ccgcggcagg ctttccccac ccagtgtatc gctaagctgg cgtgccgcgt
      121 cgtccgatac atccaggaac gcgaccttcg cgccttgacg agcgaactgg gtgacctcct
      181 cggcgccgat gccgctggcg cctccggtta ccaggacgac ccgatcgcgc aggcttgggt
      241 aggtggcgaa cccctgagtc acggtaaacc cctcagtcac ggtaaacgtt ccttcagttg
      301 actgattgct ttattaatgg ccgcctcggc cgatccgccg atgtccacga cgattggatg
      361 ctcgtctggc gccggctctt cgagagtcgc gaactggcta tctaatagcg ctggaggcat
      421 gaaatggttt tgccgcgcct tcatgcgttc ggcgatcagc gcttgcgagc cgcgcaaata
      481 gaccagcacc gtgtccggcc gcccgcccat aaggatgtcg cgataagctc gcttcagcgc
      541 ggaacaggca actacggcgg attgtcccgc agcgatcagc tcatcctccc gggcggcgat
      601 agcctgcagc caaggccacc ggtcgtcgtc ggtcaggggg atcccgacct tcatcttggc
      661 gatattcgcc ggtggatgaa acgaatcgcc ttccagcaat agccagccct tctgccgagc
      721 gagacccgcg gcgacggtgg tcttaccgct gcccgagacc cccatcacga tgactatcgt
      781 cacagcgtgc tcggtttgac atggccgcca aagccgaacc gcgtggcgga caatattttc
      841 ctggcaaaca tgggcttccg acgcgaccgg acgagggtga acagcgatgc cgtcggcatg
      901 gcagctggca cagtttcttc agtcgcggtc tccacggttc cgcctggcaa ccgtcgtcgt
      961 gcgccatttt tgcccggggc atcgatggtt gccgcgttcg cgccccaaac caccgaggat
     1021 tgactggctt tcatccgaaa acgccaaccg gcattcgaaa ggtccttatc attataggtt
     1081 cggacgaggc atatggctcg ccggacgcag cctgccacat gcccgctgca aggcaagtcg
     1141 tccatcgccg ccgaagccag cgcgcatttt tgaaccgcgg cctgctaaac cccgaaccga
     1201 ggcagatgcg ctgcaagttc ggtgtacgcg tcatcgtccg tgcggtcata ccgcaacggg
     1261 gtcacgacga ttttcctggc acgcagcgcg ctgtagtcgc tttccgggcc ctgctgcagg
     1321 tcgccgcgtt tgaagttcag ccaatgatag gacatgccgc ggggatcgac gcgggtctcc
     1381 acgtgcatgc cggcgacagc gccctcgcct tgtcgagcca gggtcacggg ccccgcctcg
     1441 gcggcaggaa tcggcgggaa attgatattc aggatggcgg ttttgctcca gccgacggcc
     1501 aacaagctgc ggattgtttc agcgcccagc gtgcgcgcgg tatcccacgg cacggcatcc
     1561 cggttcgtga acgcctggct gagcgccaac gccggcaccc ccagcatcat cgctgtcatc
     1621 gcgccgccca cggtgccgga gaacacagtc tccaggccca ggttcaggcc ccggttcact
     1681 cccgaaagca ccagttgcgg cgggcaatcc ttcatgatgt ggcacagccc catggcggcg
     1741 cagtcgccgg gtgttccggt gacaccaaac cggcgattgc cacgttcgct gatccgtagc
     1801 gcgtgatgca ggctgatggc gtgggactgg ccgctctggt cgtgttcggg cgcgacgacc
     1861 cagacctctc gggcgatgag ggcggcgatt tcctccagca cttgcatgcc cggggc
//
LOCUS       DASWQN010000487         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2289472, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000487 DASWQN010000000
VERSION     DASWQN010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289472"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VGC82_08015"
     CDS             complement(<1..198)
                     /locus_tag="VGC82_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423252.1"
                     /translation="MDIAIQSSPHLAVQIISQNDKTDARSNDGRRREKQTEAFVHISR
                     RGVLLGSAGALSAATLPFAKPA"
     gene            complement(319..627)
                     /locus_tag="VGC82_08020"
     CDS             complement(319..627)
                     /locus_tag="VGC82_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423253.1"
                     /translation="MHLIEILLPLRDNEGRPFGPEKYAAVRECLAARFGGLTAFTRSP
                     AEGTTTTGDGRTVRDDIVVFEVMAETIDAAWWRDYRRTLEGDFRQDEIVVRASAVCLL
                     "
     gene            complement(641..>2549)
                     /gene="malQ"
                     /locus_tag="VGC82_08025"
     CDS             complement(641..>2549)
                     /gene="malQ"
                     /locus_tag="VGC82_08025"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY0423254.1"
                     /translation="PPGQTFLPPVAVVQPIDGRVIVRLSKSPELQKIEWTLTLENGEL
                     RKGAAPVSRDCNNLPVLVLPAIPHGYHSLHLPDLDDRSSLIVTPGRCFLPERFNEGSR
                     AWGISLQLYLLRSEHNWGIGDFSDLATLARLTASAGCDVLGLNPLHQMFPDRPEHASP
                     YSPATRRFLNILYIDVEAIAEFSLSEARELVESEKFKAALALCRAAPQVDYTAVTRLK
                     LQALRLAYKLFGSHASETRKREFAAFVKDGGELLQSSSLFQALTHHFSEIEGSFCEWQ
                     RWPGGFRSPGSPDAIRFRMAQDDEIRFFDWSQWVADQQLAAASSVAAASGMAIGLYRD
                     LAVGCDRAGAETWSRPSDFLTSAQVGAPPDILNPAGQNWGLPPFNPVALRKQAYFPFI
                     DLIRANMRHAGGLRIDHVMGLQRLYCIPEGSPASEGAYLNYPIDDLIGILALESHRGR
                     CLVVGEDLGTVPAGFREKMAQANILSYRVLFFEQDGDHGFVGPDDYPALAVSVAGSHD
                     LPTLHSWLLGTDIELKRRLKLYPSEEETTAQRALRGEQRAAILEALELGHRATADQFS
                     AAVHRFLSRTRSVLTLAQLDDLIAEREPVNVPATSTEHPNWRRKYSASLDELSGHQTF
                     RDLVAVIGERD"
BASE COUNT          536 a          765 c          766 g          482 t
ORIGIN      
        1 ggccggtttt gcaaatggga gtgttgcggc gctgagcgcg cccgcgctgc ctaacaaaac
       61 gccacgacga gaaatgtgca cgaaggcctc cgtttgtttc tcccgccgac gcccgtcgtt
      121 gctgcgggcg tccgtcttat cgttctggct tataatctgg accgctaaat gtggacttga
      181 ttggatggca atgtccatag cgcggtcgga agatatcctg cgaattattg gttcagatgc
      241 ttgaacctgg tgcggtcgcg gaaagtcggg ttcgccagac gacatctcgg cgtggccgcg
      301 gtttgcaggc ttccggcgtc agagcagaca gacggccgag gcgcggacga cgatctcatc
      361 ttggcggaag tcgccctcca aagtccgccg gtagtcgcgc caccacgcgg catcgatcgt
      421 ttcggccatg acctcgaaca ccacgatgtc gtcgcgcacc gtcctgccgt ctccggtcgt
      481 ggtggtgcct tcggcgggtg atcgggtgaa tgccgtcaag cctccgaatc gcgcggccag
      541 acattcgcgg acggccgcgt acttctccgg cccgaatggc cggccttcgt tgtcgcgaag
      601 cgggagcaga atctcgatca gatgcatcgc ccaccgaaaa ctaatctcgc tcccctatta
      661 ccgcgacgag gtcgcgaaag gtctgatgtc cggaaagctc atccagacta gcggaatact
      721 tccgccgcca gttggggtgt tcggtcgaag tcgccggcac gttgaccggc tcacgctcgg
      781 ctatcaaatc atccagttga gccagagtta agacagacct ggttcggctc aagaagcgat
      841 ggactgccgc agaaaattga tcggccgtag ctcggtggcc aagttccagg gcctcaagga
      901 tggcggcgcg ctgctcgccg cgaagcgcgc gctgagccgt ggtctcctcc tcggagggat
      961 atagtttcaa gcgccgcttg agctcgatgt cggttccaag taaccagctg tgtagcgtgg
     1021 gaaggtcatg gctgccggcc accgaaaccg ccagcgcggg ataatcgtcc ggaccaacaa
     1081 agccgtgatc cccgtcctgc tcgaagaaga gaacccgata ggaaagaata ttggcttgcg
     1141 ccattttctc gcgaaatccg gcagggacag taccaaggtc ctcgccaacg acgagacagc
     1201 ggccccgatg gctttcaagc gcaagtattc cgatcaggtc gtcgatcgga tagttcaggt
     1261 aagcgccttc cgaggctggt gatccctccg ggatacaata aagcctttgc agccccatga
     1321 catgatcgat ccgcaagccg cccgcatgcc gcatgttcgc ccggatcaga tcgataaacg
     1381 ggaagtaagc ctgcttgcgc agcgcgacag gattgaaggg agggagcccc caattctgac
     1441 cggcgggatt tagaatgtct ggaggcgctc caacctgggc ggaagtcaaa aagtcggaag
     1501 gccgcgacca ggtttcggcg ccggcccggt cgcatcccac cgccaggtcg cgatagagac
     1561 cgatcgccat ccccgacgcc gctgcaacgc tgctggcggc ggcaagctgt tgatcggcga
     1621 cccactgcga ccagtcaaag aaccgtattt cgtcgtcctg cgccatcctg aaccggattg
     1681 cgtcaggcga tcccggcgag cgaaaacccc ccggccaacg ctgccattcg cagaacgagc
     1741 cctctatttc agagaagtga tgggttagcg cctggaacag cgacgagctc tgcaggagtt
     1801 cgcccccgtc ctttacaaag gcggcaaatt cgcgctttcg cgtttcgctc gcatgcgaac
     1861 caaagagctt atacgcaagc cggagagcct gcagcttcag gcgagtgact gctgtataat
     1921 ccacctgggg agccgcgcga cagagcgcga gcgccgcttt gaacttctcc gactcgacaa
     1981 gctcacgggc ctccgaaagg ctgaactccg caatggcctc gacgtcgata tagagaatat
     2041 tcaggaagcg ccgggttgca ggagagtatg ggctcgcatg ttccggtctg tcggggaaca
     2101 tctggtggag ggggttgagt cccaggacgt cgcatcccgc gctggcggtt agcctagcga
     2161 gcgtggccag gtcgctgaaa tcgccaatcc cccaattatg ttcggaccgc aacaggtaaa
     2221 gctgcagtga aataccccaa gcgcggcttc cctcattgaa gcgttccggc aagaagcatc
     2281 gacccggggt aacaatcaag gagctgcgat catccagatc cggcagatgc agagaatgat
     2341 agccatgagg gatagccggc agcacaagca ccggcaagtt attgcagtct cgcgacaccg
     2401 gcgccgcgcc ctttctcagc tctccgtttt ccagcgtcag ggtccattca atcttttgca
     2461 gctccggcga ttttgaaagc ctaacgatca cccttccatc aatgggctga acgacagcca
     2521 ccggaggaag gaacgtttgg cctggcggc
//
LOCUS       DASWQN010000488         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_46838, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000488 DASWQN010000000
VERSION     DASWQN010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46838"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1616)
                     /locus_tag="VGC82_08030"
     CDS             complement(147..1616)
                     /locus_tag="VGC82_08030"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEY0423255.1"
                     /translation="MPDGREPALSPDQAVAKLETLHAEAVQAQRDALTRFASGGTPPS
                     AKERARFRYPELHLEWKPKGPIPITRRAWGKFQAPGTYATTITQPAFFRPYLLEQLRP
                     LVAEYGASIRVRVGEQEMPYPYVLDRGDELAESDASPAALARHFPTPLLSAVGDEVAD
                     GLWTSQAGQALPLALFDAVRVDYSLRRLLHYTGTDWRHIQPWILLTNYQRYIDQFVRW
                     GLDELADPASPYERLIAPGGTVATRGDNPAEADVRLAAAPWHRFQMPAYHLTRSDGQG
                     GVTIVNIGVGPSNAKNITDHLAVLRPHCWLMIGHCGGLRQSQAIGDYVLAHGYLRRDR
                     ILDDLVPPEVPVPALAEVQVALQEAAVRVTGERGDALKQRLRTGTVVSYDDRNWELRW
                     SQERRRINLSRAIAVDMESGTLAAQGYRLRVPYGTLLCVSDKPLHGEIKLPGTAGAFY
                     QRAVGEHLRIGIAALGLLRCDLGALHSRKLRSFDEPPFR"
     gene            complement(1728..1803)
                     /locus_tag="VGC82_08035"
     tRNA            complement(1728..1803)
                     /locus_tag="VGC82_08035"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1768..1770),aa:Ala,seq:ggc)
     gene            complement(2063..>2803)
                     /locus_tag="VGC82_08040"
     CDS             complement(2063..>2803)
                     /locus_tag="VGC82_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0423256.1"
                     /translation="ARPVTPFEIRSLSGVVNPRRNSYEHRNNVKTVKEIPMYDQSKLS
                     ELIRFARVDAGSTVIDVYPGDGDWTRLFSDIVGPEGRVYSFVPAEVAHFKNDPVGRMR
                     TLAKEPGRENVEAVSADLVAMPEATQPADVVWLHLFYHDLHTALTQAGGATAAHFNRA
                     VYERLKPGGSYVIVDHAAAAGAGTSDAQSLHRIEPASVREEVEAAGFIMDAESTILAN
                     KDDPHSIKVFDPSIKGETDRFAYRFVKP"
BASE COUNT          475 a          900 c          937 g          491 t
ORIGIN      
        1 actccgacta ccggccgttc gaggaactgt tggctgtgct gtcgcggcca tacgatgacg
       61 atccggcgct ggtccgtttc gcgacgccgc cgctgcagga ggaacgtgtg cttcaaacct
      121 tctgcgggac ctgacggacg cctgacctac cggaacggcg gctcgtcgaa gctgcgcagc
      181 ttgcgtgaat gcagcgcgcc aaggtcgcag cgcagcaggc cgagtgccgc gatgccgatg
      241 cgcagatgct cgccgacggc gcgctggtag aacgcgccgg ccgtccccgg cagcttgatt
      301 tcgccgtgca gcggcttgtc ggaaacacag agcagggtgc cgtagggtac ccgcagccgg
      361 tagccttgcg cggccagggt gccgctttcc atatcgaccg cgatggcgcg cgacaggttg
      421 atacgccgcc gttcctgcga ccagcgaagc tcccagttgc ggtcgtcgta ggagaccacg
      481 gtgccggtgc gcagccgctg cttcaacgcg tccccccgct ccccggtcac ccgaacggcg
      541 gcttcctgga gcgccacctg cacctctgcc agcgccggga cgggcacctc cggcggcacc
      601 aggtcgtcca ggatgcggtc gcgccgcaga tagccatgcg ccagcacgta atcgccgatg
      661 gcctgcgatt gccgcaagcc gccgcaatgg ccgatcatca gccagcaatg cggccgcagc
      721 accgcgaggt ggtcggtgat gttcttggcg ttggacggcc cgacgccgat gttgacgatg
      781 gtgacgccgc cctgcccatc ggatcgcgtc aggtggtatg caggcatctg gaagcggtgc
      841 cagggcgccg cggcaagccg aacgtcggcc tccgccgggt tgtcgccccg ggtggcgacg
      901 gtgccgcctg gcgcgatcag ccgttcgtag gggctggccg gatcggcaag ctcatccagg
      961 ccccagcgca cgaactggtc gatatagcgc tgatagttgg tcagcaggat ccacggctgg
     1021 atgtgacgcc agtcggtccc ggtgtagtgc agcaggcggc gcagcgagta atcgacccgc
     1081 accgcatcga acaacgccag cggcaaagcc tgtccggcct gggaggtcca tagcccgtcg
     1141 gccacctcgt cgcccaccgc ggacagcaag ggcgtgggga aatggcgcgc cagcgcggcc
     1201 ggggaggcgt cgctttcagc aagctcatcg ccgcgatcga gcacataggg atatggcatc
     1261 tcctgctcgc cgacccgcac gcggatcgag gcgccatatt ccgccaccag cggccgcagt
     1321 tgctcgagca gataaggacg gaagaacgcc ggctgggtga tcgtggtggc ataggtgccg
     1381 ggtgcctgaa acttgcccca ggcgcgacgg gtaatcggga ttggtccttt cggtttccac
     1441 tccagatgca gctcgggata gcggaagcgc gcacgctctt tggcgcttgg tggagtgccg
     1501 cccgaggcaa agcgcgtcaa tgcgtcacgc tgggcttgca ccgcctcggc atgcagtgtc
     1561 tccagcttag ccaccgcctg gtccggcgaa agtgcgggct cgcgtccatc gggcatagga
     1621 tcaagacttc cagtggcggc cggatgttcg tggagaccct cgcggtccgg gtgcaagtgc
     1681 cactgcgcga ggccgtgggc cgatcatgcg aggtattgcc ctggcgttgg tggagctgag
     1741 gggaatcgaa cccctgacct cctgaatgcc attcaggcgc tctcccaact gagctacagc
     1801 cccaacccgc gaggcagggg gtatagccac ctcgttccaa cggatcaagg gcaagagtag
     1861 tgggtcagtt tgaattttgg ccggagttgg ccgagtccag cctggcggct ttggggcgag
     1921 ttgataggga aagctgccgt ccgccagaca cgcccgttcg tcggctttcg cccagacccg
     1981 gtcgttcgcg gccaatttca gattccccaa aaccagtcag tcaccgttta ggcacactgg
     2041 cagcaaggcg cgccgggacc tgtcagggct tcacgaaccg gtaggcaaag cgatcggtct
     2101 cgcccttgat cgagggatcg aacaccttga tcgagtgcgg atcgtccttg ttcgcgagga
     2161 tggtgctttc cgcgtccatt atgaagccgg ccgcctccac ctcctcgcga acggatgcag
     2221 gctcaatccg atgcagcgac tgggcgtcgc tcgtgcccgc cccggcagcg gcggcgtggt
     2281 ctacgatgac gtaagaccca ccgggcttca gccgctcgta gacagctcga ttgaagtggg
     2341 ccgccgtcgc gcccccggcc tgggtcagcg cagtgtggag atcatggtag aacaggtgca
     2401 gccatacgac atccgctggt tgcgtggcct ccggcatcgc cacgaggtcc gccgagacgg
     2461 cttcgacgtt ctcgcggccc ggctccttcg caagcgtccg catgcggccg accggatcgt
     2521 tcttgaagtg ggcaacttcg gccggcacga agctgtagac ccgtccttcg ggtcccacga
     2581 tgtcggagaa gagacgggtc cagtcgccgt cgcctgggta aacgtcgatg acggtggagc
     2641 ctgcatcgac tcgtgcgaac cggatcaact cggatagctt ggattggtcg tacatcggga
     2701 tctccttcac ggtcttgacg ttgttccggt gctcatagga atttctcctt ggatttacca
     2761 cgcccgacag gctccgaatc tcgaatggag tgaccggccg ggc
//
LOCUS       DASWQN010000489         7099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_838515, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000489 DASWQN010000000
VERSION     DASWQN010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838515"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VGC82_08045"
     CDS             <1..571
                     /locus_tag="VGC82_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423257.1"
                     /translation="RPPDAGRPATPPAAPEGNSHPTATPSAAPATPMSAPRPSTVALA
                     EREKNLRNAGVPAAPIIAVSRPFEDPVPPELASDGIDLVTLMFNYHDLGFMGVDRAAM
                     NRAVFRALKPGGLFVVADHSGRTGTGISESGTLHRIEKEFLRKEVEAAGFKLLEEGDF
                     LRNPNDPRDKNTPDPPQPKDEFVLKFVKQ"
     gene            778..1980
                     /locus_tag="VGC82_08050"
     CDS             778..1980
                     /locus_tag="VGC82_08050"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0423258.1"
                     /translation="MTGPLDGIRVLDLTTVQMGPWCTRIVADLGASVIKVEAPEGDSS
                     RYTGVPRHRGMSGSFQHNARSKRSIAIDLKQPEARQAVLRLVPTVDAFASNIRSAALA
                     RLGLDYESVRKANPSIVYLSMVGYGSAGRYAGRPAYDDLIQAAAAIPMLLQRSTGQPR
                     FIPMAAIDRIVGSAAANALLAGLLARARTGIGQMIEVPMFETIAQFVLSEHMQGQTFD
                     PPTSPAGYKRTLSPHRRPYPTKDGFIAVLPYNDGQWRRFFEVAGKAHILQSDPRFADI
                     ASRTANIDSLYEMVGEELKHRTTDEWLVLLSRHDIPCMRPHTLETLLEDPHLADAGFF
                     RWEEHPSEGRIRTMREPSTWSETEPPTGRFAPRLGQHTREILAEAGFDADAIDSLIAR
                     QIAKTDTA"
     gene            2079..3092
                     /locus_tag="VGC82_08055"
     CDS             2079..3092
                     /locus_tag="VGC82_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423259.1"
                     /translation="MQKHFRLVALAALLLLFGLAPPARAESNQVRIVMPYGLAYLPTY
                     IVVDGGLIEKHAKAAGLGDIKVTLLHMASGPASSDLLLAGDADLAMGGFGPAFVLWDK
                     TRGAGKVRIVTPQSGSPIWLLTNDPHIQSIRDFGPNDRIAVSAIKVTAQALSLQMAAA
                     KEWGWDQRFRLDSNMVSISNPDGMAMLLSGAGSVRTQAAIIPFSVEEMESGKVHMVMS
                     SEDYLEPGSSIAVVYAAAGFHDGSPKLYAATAAAYEEAFDFIHRHPHEAAEIYVAREP
                     QKRELAWIEAMIVNPKLIQYSSTPRGLKKHADFMYAAGTLKSKPDSWKDLVWDNMWSK
                     DGS"
     gene            complement(3162..3509)
                     /locus_tag="VGC82_08060"
     CDS             complement(3162..3509)
                     /locus_tag="VGC82_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423260.1"
                     /translation="MASWNSTKIRIGSLVFRTWTACAVLAAYVYGWLYHPEMLTWWKR
                     TTTSIIEDTCRLLPYPWGDRIESTLGNFGLWVQVTLAIIGFRFLMWLLISLLRRIWGA
                     RNQGAEKSPPSAA"
     gene            4008..5240
                     /locus_tag="VGC82_08065"
     CDS             4008..5240
                     /locus_tag="VGC82_08065"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY0423261.1"
                     /translation="MLTRRAATVLLTGIAAAPSGARGASPRPAAVFYNAVGPDLTCWH
                     PDFDNAALSRRDGVTLPALVQYAWRHPAKLILYVASSNFVPMGGPDGKHHLTAFTIDP
                     DTGSLRQFGASVPIRARPIHISVDSGGNWLLTAYNLPSAMSVHPLDSDGAIGAEVKQA
                     AAIDCGIYAHQVRMMPANRSLVLVTRGNDATAAKPEDPGALKVKALKSGQITDIASVA
                     PGSGLGFGPRHVDFHPTKPWMYVSVERQNQLQMYRLRGDDLETAPAYVATTLSEPTPR
                     PVQMAGPIHLHPNGRFVYIANRASGLIETGGRKTAAGGENTVAIFSINQATGEPTLTQ
                     AVDTHGFHPRTFSIDPSGRMLVVANLQEMPVGDGTKIQPATLSAYKIGDGGSLTFVRA
                     YDIETKGLTQWWSGFIPL"
     gene            complement(5348..6421)
                     /locus_tag="VGC82_08070"
     CDS             complement(5348..6421)
                     /locus_tag="VGC82_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0423262.1"
                     /translation="MEFGIGIPTSGASWKVAQRAEELGFTHAWFYDTQMLVGDPFVAM
                     GAAALKTSRIRLGTGVLVPTNRIPAVTASAFATLNAMAPGRIDFGVGTGFSARRAMGL
                     GAMKLADMEEYIRVVYALLNNEMTEAEVDPGVRKKIKFLNPELGLINIKDPIRLHISA
                     LGPKGRQMTARHGAAWKTVMSGVDDGLAALTGMKKTWTDSGRPGGDLYSSAWIWGCLL
                     RDGETLDSPRVRAQAGPRAAVLLHRAADVDMEGWINTSPVPDSVKDVLQGYIEAARRF
                     EPADARYLLNHTGHCIAVKPEEERFITAELIRQTTFTGVEQDIKQRMEAMRDGGLSQL
                     VIPIVPGHESAIEDWGRIAKAFR"
     gene            complement(6522..>7099)
                     /locus_tag="VGC82_08075"
     CDS             complement(6522..>7099)
                     /locus_tag="VGC82_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423263.1"
                     /translation="PAGELYISDGYGNARVHKYSPDGKLLHSWGGPGTNPGEFNIAHN
                     ITCDGDGWVYVADRENHRVQVFDGNGRYETQWNNMHRPSGLFMTPGKCPLCYIGEIGP
                     HLPVNRALPNIGPRISVLDHTGKLLARVQAEPARGTGPGQFISPHGLAVDSKWNLYVG
                     EVAYTSWSSSFPDVPKPKRLRCLQKLACVEV"
BASE COUNT         1320 a         2346 c         2116 g         1317 t
ORIGIN      
        1 ccggccgccc gacgccggcc gtccggccac gcctcctgcc gccccggagg gcaacagcca
       61 tccgacggcc acgccaagcg cggcgccggc gacgccaatg agcgcgccac gtccgtccac
      121 cgtcgccttg gctgaacgcg aaaagaattt gcgaaacgct ggcgtcccag ccgcgccgat
      181 catcgcggtg tcccggccat tcgaggaccc ggttcccccg gaactggcgt cggacggcat
      241 cgacctcgtg accttgatgt tcaactacca cgacctcgga tttatgggtg tggaccgggc
      301 ggcaatgaac cgggccgtgt tccgcgcgct gaaaccaggt gggttgttcg tggtggccga
      361 ccactcaggc cggaccggca ccggcatctc cgagtctggt actttgcatc gcatcgaaaa
      421 agagttcctc cgcaaggagg tcgaagccgc cggattcaag ctgttggagg agggcgactt
      481 cctgcgtaat cccaacgatc ccagggacaa gaacacaccc gacccgccac agccaaagga
      541 cgaattcgtg ctcaagttcg tcaagcagtg atgctgggct ttatcgatgc cgagccgcgg
      601 gccgttgttc ctgtcgtgct tgtgccttcc cggcatgcgg ccgatagttg accggacgca
      661 accctcgaca gcggagacac gtcacgatgc caacctggtt cgatgccgcg ggaaagcgac
      721 tttcatcgcg gcagccacgc ggacgatccc gcccgacgac aacgacctga gcctgcaatg
      781 accggtccac tcgacggcat tcgggtgctc gatcttacca cggtccagat ggggccctgg
      841 tgcacgcgca tcgtcgccga tctcggcgcg tccgtaataa aggtggaggc gccggaaggg
      901 gattcgtcgc gatataccgg cgtgccccgg catcgcggca tgagcggaag ttttcagcat
      961 aatgcccgca gcaagcgcag catcgccatc gacctcaagc agccggaggc ccggcaagcg
     1021 gtgctgcgcc tggtgccgac cgtcgatgcc tttgcctcaa acatccggtc ggctgcgctc
     1081 gcgcgcctcg ggctcgacta tgagtcggtt cgaaaggcaa atccgtccat cgtctatctc
     1141 agcatggtcg gctatggcag cgccgggcgt tatgcgggac gtccggctta cgatgacctg
     1201 atccaggcgg ccgcggcgat tccgatgctg ctgcaacgat ccaccggtca gccgcgcttc
     1261 attccaatgg cggcgatcga tcgtatcgtc ggttcggccg ccgcgaacgc gttgctcgca
     1321 gggctgctcg cccgcgcgcg taccggcatc ggccagatga tcgaggtgcc gatgttcgaa
     1381 accatcgcgc agttcgtgct gtcggaacac atgcagggcc agacgttcga tccgccgacc
     1441 agccccgccg gctacaagcg cacgctttcg ccgcatcgcc ggccatatcc gaccaaagac
     1501 ggcttcatcg ccgtgctgcc atacaacgat ggccagtggc gccgcttctt cgaggtcgcg
     1561 ggcaaggcgc acattctgca aagcgaccca cgcttcgccg acattgccag ccgcacggcc
     1621 aatatcgatt cgctctacga aatggtcggc gaggaactga agcaccgcac gacagacgaa
     1681 tggctggttc tgctgtcgcg ccacgacatc ccctgcatgc gtccgcatac gctggaaacg
     1741 ttgctggagg acccgcatct tgccgatgcg ggtttcttcc gttgggaaga gcacccctcc
     1801 gaaggccgca tccgcaccat gcgcgaaccc agtacctggt cggaaaccga accgccaacc
     1861 ggccgcttcg ccccgcgcct gggccagcat acccgcgaga tcctcgccga ggccggtttc
     1921 gacgccgacg ccatcgactc cttgatcgct cggcagatcg ccaagaccga cactgcatga
     1981 tctcgttcga cgccccctga ccgggccttt ctgaacgtcg ggacgtttct cccgagggca
     2041 atcctccttt aggatcagtc ccaagggagg aaaccacaat gcaaaagcac tttcgcctgg
     2101 tcgctctcgc cgccctgctt ctgctgttcg gcttggcgcc accggccagg gcggagtcca
     2161 accaggtgcg tatcgtcatg ccctatgggc tggcgtacct gcccacctat atcgtggttg
     2221 atggtggcct gatcgaaaag cacgccaagg cggcgggttt gggcgatatc aaggtaacgt
     2281 tgctccatat ggccagcggt cccgcgagca gcgatctgtt gctggccggt gacgccgacc
     2341 tggccatggg cgggttcggt cccgccttcg tcctgtggga caagacccgt ggcgccggca
     2401 aagtgcgtat cgtgacgcct cagtctggtt cgccgatctg gctgctgacc aacgatccac
     2461 acattcaaag catccgggac ttcggtccca acgatcgtat cgccgtcagc gcgatcaagg
     2521 tgacggcgca agcgctgtcg ctgcagatgg ccgcggccaa ggaatggggc tgggaccagc
     2581 ggttccggct ggactcgaac atggtttcga tctccaaccc ggacggaatg gcgatgctgt
     2641 tgagcggcgc cggatcggtc aggactcagg cggcgatcat cccgtttagc gtcgaagaga
     2701 tggaatccgg gaaggttcat atggtgatga gttcggaaga ctacctggag ccgggttctt
     2761 cgatcgccgt cgtatatgcc gccgccggct ttcatgacgg cagccccaag ctgtatgccg
     2821 ccaccgccgc ggcctatgaa gaggcgttcg acttcatcca ccggcatccg cacgaagcgg
     2881 cggaaatata cgttgcgcgc gaaccgcaga agcgcgagct ggcctggatc gaggcgatga
     2941 tcgtcaatcc caagctgatc cagtatagtt ccacgccaag gggactgaag aagcacgccg
     3001 acttcatgta tgccgccggc acgctgaagt ccaagccgga tagctggaag gatctggtct
     3061 gggacaacat gtggagcaag gacggcagtt gatcgcgtcg cgcgccggat accgtgccgc
     3121 gaaagtggct atcggatccc ggccggaagc gagcaatcaa cttacgctgc tgacggcggt
     3181 gatttctccg ctccttggtt acgtgcgccc cagatgcgcc gcagaagcga tatcaggagc
     3241 cacatcagaa aacggaaccc gatgatggcg agggtgacct gcacccacag gccgaaatta
     3301 cccagtgtcg actcgatacg gtcgccccag gggtatggca gaaggcggca cgtatcctcg
     3361 atgatcgagg tggtcgtccg cttccaccag gtaagcatct ccgggtgata gagccatccg
     3421 taaacataag ccgccagaac ggcgcaagcg gtccatgtcc ggaaaacaag gctgccaatc
     3481 cggatcttcg tgctgttcca ggaagccacg gctggtgcct cgtgcggtct caggcttgaa
     3541 gtcgctttcg tccgaatcgt tcctcggatc ccggagctca tgaggttacg atcatgcagc
     3601 cgttggtcag tcgagcctac gcgctttcct cctccagcac gagccgatgc aaggtctcgc
     3661 acagcgcgtc cccggtcatg cgagaaggac cgagaccagc cggtgggcaa ccatcagcga
     3721 agctgcctcc gccgccgaca gtggacgtcc ggtcgggtcg ccctgcactt cgctgcggcc
     3781 ccggcgcgta ggcggtcgat ttgctcccgc gtatcgacgc ctctggttat gcggactcct
     3841 accgccttca gttcctgaat tacttgcatg cgatatcagt attttcaagg aacaaatatt
     3901 tctcctgaat gcttgccatc gtcagcggcg tgttgccacc ctcccgcggt cggtacagga
     3961 taagcataac gtccaccccg aaatccttcg cagaaaggac aacacaaatg ttgacgcgcc
     4021 gcgccgccac cgtgctgctg accggcatag ccgctgcgcc ttcgggcgca agaggggcca
     4081 gcccgcgtcc cgccgcggtc ttctacaatg ccgttggacc tgatctgacc tgctggcatc
     4141 cggacttcga caacgccgca ctgtcgcgac gggacggcgt gacgctaccg gcgttggttc
     4201 agtatgcttg gcgccatccg gcgaagctta ttctgtatgt cgcctccagc aacttcgtcc
     4261 cgatgggtgg tccggatggc aaacatcacc tgaccgcgtt caccatcgac ccggacaccg
     4321 gctcgctccg ccagttcggc gcttcggtgc cgattcgcgc ccgcccgatc cacatttccg
     4381 tggacagcgg cggcaactgg ctgcttaccg cctacaacct tccaagcgcc atgtccgtcc
     4441 atccgcttga ttcggacgga gccattggcg cggaggtcaa gcaagccgcc gcgatcgatt
     4501 gcggcatcta cgcccatcag gtccgcatga tgcccgccaa tcgttcgttg gtgctggtta
     4561 caagaggcaa cgatgccacg gccgccaagc ccgaggatcc gggcgcgctg aaggtcaagg
     4621 ccctgaagag cggacaaata accgacattg cctccgtcgc acccggatct ggcctgggtt
     4681 ttggaccgcg gcacgtcgat tttcatccta cgaagccgtg gatgtacgtg tcggtggaac
     4741 gacagaacca gctccagatg taccgcctgc gcggcgatga tctggaaacg gcacctgcct
     4801 acgtcgctac caccttgagc gagcccactc cgcgtcccgt acagatggcc ggccctatcc
     4861 atctccatcc gaacggccgc ttcgtgtata tcgccaaccg cgccagcggc ctgatcgaga
     4921 ccggaggcag aaagaccgct gccggcggcg agaacaccgt tgcaattttc agcatcaacc
     4981 aggctacagg cgagcctacg ctgacccagg cggtcgatac gcacggcttt catccgcgca
     5041 ccttctcgat cgatccgtcc ggccgcatgc tggtcgtggc aaacctgcag gagatgcccg
     5101 tcggcgacgg cacaaaaatc cagccggcga ccctgtcggc ctataagatc ggcgatggcg
     5161 gcagccttac attcgtccgc gcttatgaca tcgagacgaa aggcctgacg cagtggtgga
     5221 gcgggtttat tccgctgtag aagccgttac cgtccagcct ccgtccacac tgccaaagct
     5281 tatagcacgc cattattttc tacgcagatt cacgcctccg gacctttcgg ctctcgggcg
     5341 atccgcgcta gcgaaacgct ttggcgatcc ggccccagtc ctcgatcgcc gactcgtgcc
     5401 cgggcacaat gggaatgacg agctgcgaca atccaccgtc gcgcatcgcc tccatccgct
     5461 gcttgatgtc ctgctccacc ccggtgaacg tggtctggcg gatcagctcg gccgtgatga
     5521 aacgctcctc ctccggcttc accgcgatgc aatggccggt gtggttcagc aggtagcggg
     5581 catcggcggg ctcgaaccgg cgtgccgcct cgatatagcc ctgcagcaca tcctttacgc
     5641 tgtccggcac cggcgatgtg ttgatccagc cctccatgtc cacgtccgcc gcacgatgca
     5701 gcagcaccgc ggcacgcggg cctgcctggg cgcgaacccg ggggctgtcc agagtctcac
     5761 cgtcgcgcag caggcacccc cagatccagg ccgaggaata caggtcgccg cccggccgcc
     5821 cgctgtccgt ccaggtcttc ttcatgcctg tcaatgccgc cagcccgtca tccacgccgg
     5881 acatcacggt tttccaggct gcgccatggc gcgcggtcat ctgccgcccc ttgggcccca
     5941 gcgccgagat atgcagccgg atcgggtcct tgatgttgat cagacccaac tcggggttaa
     6001 ggaactttat ctttttccgc acgcccggat cgacttcggc ttcggtcatc tcgttgttca
     6061 gcagggcata gacgacgcga atatactctt ccatgtcggc aagcttcatc gcgcccagcc
     6121 ccatcgcccg ccgggcggaa aagccggttc ccacgccgaa gtcgatccgc cccggcgcca
     6181 tcgcgttcag ggtcgcgaag gcggatgccg tgaccgccgg aatccggttc gtcggcacca
     6241 gaacgccggt gccaaggcga atgcgcgagg ttttcaacgc cgccgccccc atcgcgacga
     6301 acggatcgcc caccagcatc tgcgtgtcgt agaaccacgc atgagtgaag cccagttcct
     6361 ccgcccgctg cgccaccttc cagcttgctc cggacgtcgg aatgccgata ccgaattcca
     6421 tggatttcgc tccccctggt tttaaggaac aaccgtatcg accggttcgg cggcgtcaac
     6481 cggcggtgaa cgcagggatt aagcgggcct gatccacgac atcagacctc gacgcaagca
     6541 agcttttgca ggcaacgcag gcgtttcggc ttcggcacgt cagggaacga actcgaccag
     6601 gaggtatagg cgacctcacc gacataaagg ttccacttcg aatcgacggc cagtccatgc
     6661 ggcgaaatga attgtcccgg tccggtgccg cgtgccggtt cggcttgcac ccgagccagc
     6721 agtttcccag tgtggtccag cacgctgatc cgtggcccga tattcggcaa ggctcggttt
     6781 accggcagat gcgggccgat ctcgccgatg tagcacaatg ggcatttgcc gggcgtcatg
     6841 aataacccgc tgggacggtg catgttgttc cactgcgttt cgtatcgtcc gttgccgtcg
     6901 aatacttgaa cccggtggtt ctcccggtcc gctacataga cccagccatc gccgtcgcag
     6961 gtgatgttgt gcgcgatgtt gaactcgcct gggtttgttc cggggccgcc ccaggagtgc
     7021 agcagctttc cgtccggcga atatttgtgc acgcgggcgt tgccgtagcc gtcggagatg
     7081 tagagctctc cggcgggtg
//
LOCUS       DASWQN010000490         2659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1630077, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000490 DASWQN010000000
VERSION     DASWQN010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630077"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VGC82_08080"
     CDS             <1..1065
                     /locus_tag="VGC82_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="depolymerase"
                     /protein_id="HEY0423264.1"
                     /translation="LIVSLCLAGAASAADPLRRYPVDPTQISVAGISSGAFMANQLHV
                     AHSADIMGIGVVAGGPFGCAVADTNDTGVIALISIATGACMQAPTRLLRAETYAERVR
                     TFAAQGWIDPVENLTRARLYAFTGQSDQVVNSKSVRRGVAVYAALGLPAALFRDDDLP
                     DPGAGHSWVTQSFGGDCELNTDPYINKCNYDQAKDILSTIYGPLKDKATQLSGHIVEF
                     DQHQFVGDGDTKSNGLWDTGYLYVPAACEAASGHCRLHVVLHGCRQSAQKLHDTFYRH
                     IGVNEWADTNDIIVLYPQARSVEVRDFKKPQPSDIFNINPEGCWNWWGYAHDSRYLFK
                     DGVQVGAIWAMVQRVTGRGN"
     gene            complement(1072..1953)
                     /locus_tag="VGC82_08085"
     CDS             complement(1072..1953)
                     /locus_tag="VGC82_08085"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY0423265.1"
                     /translation="MSAHRWPPRLCPEIEAALGAEIRAARRTAATPPIAGADPKPRWT
                     LRRLVAFVRERFARRGWRETVRSALRRLDLSWKKAKKLLGRADPERRKSFIGQIDALL
                     EGARDERHTLVYLDEAHIHQDVDLGHGWCERGQRFYVASSSPGLAAKVSFYGLYLYNE
                     GQVRIWSYARANGEHPIDVLRRLRAEIPEGKLIVIWDGAPYHRAKAVWAAAASLNIHL
                     EPLPGYSPDLMPVEALWRWVREDVTYHHCHTTAEDLIRRVAAFKAQLNQDAYAIADRL
                     WVKDCLDPEEEKLRFSR"
     gene            complement(2000..2146)
                     /locus_tag="VGC82_08090"
     CDS             complement(2000..2146)
                     /locus_tag="VGC82_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423266.1"
                     /translation="MLRVDYDRWGQTPADLRHMALNAAHTRTRERMLALHGLQNAPAA
                     VGKP"
     gene            complement(2254..2643)
                     /locus_tag="VGC82_08095"
     CDS             complement(2254..2643)
                     /locus_tag="VGC82_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY0423267.1"
                     /translation="MDLAVAVQFIRFGIVGIVGLAVDTATVYGFRWSLGLYGAGLLAY
                     LFAASVTWLLNRMWTFRGRGNVPAHRQWALFLAANLVGFVLNRGTYILLVTLLPIAAA
                     QPVIATASGSLAGMFVNFALSRRIVFR"
BASE COUNT          532 a          830 c          824 g          473 t
ORIGIN      
        1 ctcattgtca gtctttgtct cgccggagcg gcctcggcgg ctgatcctct caggcgctac
       61 ccggtggacc cgacacaaat ttccgtcgcg gggatatcct ccggcgcttt catggcgaat
      121 caactgcatg tcgcgcattc ggcggacatt atgggtatcg gcgtggtcgc cggagggcca
      181 ttcggctgcg cggtcgccga caccaacgac accggcgtta tagcgttgat cagcatcgcc
      241 accggcgcct gcatgcaggc gccgacgcgt ctgctgcgcg ccgagaccta cgccgagcgc
      301 gttcgcacct ttgccgcgca gggttggatc gacccggtcg aaaatctgac gcgggcccgg
      361 ctctacgcct tcaccggtca gtccgaccaa gtggtaaact ctaaatcggt ccgccgcggc
      421 gtcgcggtgt atgccgcact gggcctgccg gcggcgctgt ttcgggacga cgacctgccg
      481 gacccgggcg ccgggcactc gtgggtgaca caaagcttcg gcggggactg cgaactcaac
      541 accgacccat acatcaacaa atgcaactat gaccaggcga aggacatcct ctccacgatc
      601 tacggcccgc tgaaggacaa ggcgacgcag ctttccggcc acatcgtcga attcgaccag
      661 caccaattcg tgggtgacgg cgacacgaag agcaacggcc tgtgggacac cggctatctc
      721 tacgtgcccg ccgcgtgcga ggcggcttcc ggccactgcc gattgcacgt cgtgctgcac
      781 gggtgccgcc agtcggcaca gaagctgcac gatacgttct accgccatat cggcgtgaac
      841 gagtgggcgg ataccaacga tatcattgtg ctctatccgc aggcgcgctc ggtcgaggtc
      901 agagacttca agaagccgca gccgtccgac atcttcaaca tcaatccgga ggggtgctgg
      961 aactggtggg gttacgccca cgacagccgc tacttgttca aggacggcgt tcaggtcggc
     1021 gcgatctggg cgatggtgca gcgggtgacc gggcgcggga attaaccgcg tctatctcga
     1081 gaaccgtagt ttctcctctt cgggatcgag gcagtccttg acccaaaggc gatcggcaat
     1141 ggcgtaagca tcctggttca gttgcgcctt gaatgcagca acgcgccgga tgagatcttc
     1201 agcggtggta tggcaatgat gataggtgac gtcctcgcgc acccagcgcc agagcgcctc
     1261 gaccggcatc aggtcaggac tgtagcccgg cagcggctcg aggtggatgt tcaggcttgc
     1321 cgccgccgcc cagaccgcct tggcccgatg atacggcgcg ccatcccaaa tcacgatgag
     1381 cttgccctcg gggatctcgg cgcgcagccg acgcagtacg tcgatgggat gctcgccatt
     1441 ggctcgcgca taggaccaga tccgcacctg gccctcgtta tacagataca ggccatagaa
     1501 cgagactttg gcggccaggc ccggcgacga agaggcgacg tagaagcgct ggccgcgctc
     1561 gcaccagcca tggccgagat cgacatcctg atggatgtgc gcttcatcga gatagaccag
     1621 ggtatgccgc tcatcgcgcg caccctccag taacgcgtcg atttgcccga tgaagctttt
     1681 gcgccgctcg ggatcggcgc gtccgagcaa tttcttggcc ttcttccacg acaggtccaa
     1741 ccgccgcagg gcgctgcgga cggtctcacg ccagccgcgg cgtgcaaacc gttcgcgtac
     1801 gaacgcaacc agacgccgca gtgtccaacg tggttttgga tcggcccccg ctatgggtgg
     1861 tgtggctgct gtgcgccgag cggcacggat ttctgcgcca agtgctgctt cgatctccgg
     1921 gcaaaggcgg ggcggccacc ggtgcgccga tatgacagcg cctcggggcc gtgttcattg
     1981 tagctgtgca gccagtccat tacggtttgc cgacggcggc cggtgcgttc tgcaacccgt
     2041 gcaaggccag cattcgctcg cgggtgcggg tatgggcggc attcaaggcc atgtgccgca
     2101 ggtcagccgg ggtctggccc cagcgatcat aatcaacgcg tagcatcagt tctgacgtga
     2161 tcctatcaag ggaaactcgt gatgtgagaa cagctcgcca cacagccaag ggctcaacta
     2221 gcggtctcaa actggacacg gtttagcaaa atcttagcga aacacaatcc tgcgagataa
     2281 ggcaaagtta acaaacattc cagcgagcga gccggacgcg gtcgcgatga cgggttgggc
     2341 cgcagctatg ggcagcaagg tgaccagcag aatgtaagtt cccctgttca acacgaagcc
     2401 gaccaggttg gcggcgagaa acagcgccca ttggcggtga gctggaacgt tcccgcggcc
     2461 ccggaaggtc cacatgcgat tgagaagcca cgtcacactt gcggcaaaca gataagcgag
     2521 caatccggcc ccatagagac caagtgacca gcggaacccg tataccgttg ccgtgtcgac
     2581 cgcgaggccg acgatgccaa caatgccgaa tcggataaac tggaccgcaa cagccaaatc
     2641 catttcacgg ctcgttctt
//
LOCUS       DASWQN010000491         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2157875, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000491 DASWQN010000000
VERSION     DASWQN010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2157875"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1071
                     /locus_tag="VGC82_08100"
     CDS             70..1071
                     /locus_tag="VGC82_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0423268.1"
                     /translation="MPPLLDVRNLCTEFRTGRGIVRAVDRVSYTVEAGETVAIVGESG
                     SGKSVGAMSILRLIPDPPGRITNGEVVFAGRDLMKLPDEAMRRIRGGEIGMVFQEPMT
                     SLNPVLTVGRQISEVLEQHRGATPAAARQRAQHLLGLVGIADPARRLRQYPHQLSGGM
                     RQRVMIAMALTCDPKLIIADEPTTALDVTIQAQILELMKDLTRRLDIALIIITHNLGV
                     VARYASRVNVMYAGRIVEAGSAAAIYHHPRHPYTIALLRSVPRLDRPRQMRLDPVDGQ
                     PPDLTRLDKGCAFRPRCRFAAAACAEARPVLAPVAEGGHVAACFRSHEIAAEAGAAA"
     gene            1068..2081
                     /locus_tag="VGC82_08105"
     CDS             1068..2081
                     /locus_tag="VGC82_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0423269.1"
                     /translation="MTTIVPSPRPLLEVKGLRMHFPVTEGMFMRRVVAEVKAVDGVDF
                     TVRRGETFGLVGESGCGKTTTGRCILLLENPTAGEIIYDGIPIADSGRKKLPRKDLLA
                     LRRRIQVIFQDPYSSLNPRMTVGAIINEPMQVHGIEPDATRRLDRVRELLSICGLDPH
                     FADRYPHEMSGGQRQRVGIARALALNPEFIVCDEPVSALDVSIQAQVVNLLEDLRERF
                     GLTYLFIAHDLSVVRHLCQRVAVMYLGRVVEMADCDTLFDAPLHPYTQALLAAVPVPD
                     PSIERTRIFRPVQGEVPSPMNPPSGCVFHPRCPIAVESCKHTRPEPREVRPGHSVACS
                     QVH"
BASE COUNT          389 a          718 c          710 g          394 t
ORIGIN      
        1 cgggccgccg ataactaacc gcagaacaat aatatcaggg tttcacggaa aacgttcggg
       61 agcgagccat tgccgccact gctcgacgtc cggaatttat gcaccgagtt tcgcaccggg
      121 cggggaatcg tgcgtgctgt cgatcgggtc agctataccg tcgaagcggg cgagaccgtc
      181 gccatcgttg gcgaaagtgg atcgggcaaa tcggtgggtg cgatgtccat cctgcgcctg
      241 atcccggacc cgccgggacg gatcaccaac ggcgaggttg tgttcgccgg ccgcgacctg
      301 atgaagctgc cggacgaggc gatgcgtcgg atccgaggcg gtgaaatcgg catggtgttc
      361 caggagccga tgacatcgtt gaacccggtc ctgacggtcg gtcgccagat ctccgaagtg
      421 ctggagcagc atcgcggagc aacccccgcc gccgcccgcc agcgcgccca acaccttctg
      481 ggtctggtcg gcatcgccga tcccgcgcgc aggctgcggc agtacccgca tcagttgtcg
      541 ggcggcatgc gccagcgtgt catgatcgcc atggcactga cctgcgaccc aaagctgatc
      601 atcgcggatg agccgacaac cgcgctcgat gtgacgatcc aggcgcaaat cctggaattg
      661 atgaaagacc tgacgcgccg gctggacatc gccctgatca tcatcactca taaccttggc
      721 gtcgtcgccc gctatgccag ccgggtgaac gtcatgtacg ccggccgcat cgtcgaagcc
      781 ggctccgccg cggcgatcta ccatcacccg cgccacccct acacgatcgc cctgctgcgc
      841 tcggttcccc gcctggatcg gccgcggcag atgcgcctcg acccggtcga tggccagccg
      901 cccgacctga cccggttgga caaaggctgc gcattccgcc cgcgctgccg cttcgccgcc
      961 gctgcctgcg ccgaagcgcg gcccgttctc gcacccgtgg cggaaggcgg tcatgtcgcg
     1021 gcctgttttc gcagccacga aatcgccgcc gaagccggag cggccgcatg accaccatcg
     1081 tgccatcgcc gcggccgttg ctggaggtca agggcctgcg catgcacttc ccggtcaccg
     1141 agggtatgtt catgcgccgt gtggtggcgg aggtaaaagc ggtggacggc gtggatttca
     1201 ccgtgcggcg cggcgagacg ttcggcctgg tgggcgagag cggctgcggc aagaccacca
     1261 ccggccgctg tatcctgctg ctggaaaatc cgaccgccgg cgagatcatc tacgacggca
     1321 tcccaatagc cgactccggg cgcaagaaat taccgcgcaa ggatctgttg gcgttgcgcc
     1381 ggcgtatcca ggtcatcttc caggacccat acagctcgtt gaatccgcgc atgacggttg
     1441 gcgccatcat caacgaaccg atgcaggtgc atggtatcga gcccgacgca accaggcgtc
     1501 tggaccgggt tcgtgaactg ctgtcgatct gcggcctcga tccacatttc gccgatcgct
     1561 atccgcacga gatgtcaggc ggccagcggc aacgcgtggg tatcgcccga gcgctggcgc
     1621 tcaatccgga gttcatcgtg tgcgacgagc cggtctcggc gctggacgtg tcgatccagg
     1681 cgcaggtggt caacctgctt gaagatttgc gcgaacggtt tggcctgacc tatttgttca
     1741 tcgcccacga cctgtcggtt gtgcggcacc tctgccagcg cgtggcggtg atgtatcttg
     1801 gccgcgtcgt cgagatggcc gactgcgaca cgctgttcga cgcgccactg catccgtaca
     1861 cacaagcctt gctcgcggcg gtgccggtcc ccgatccgtc gatcgagcga acccgcatct
     1921 tccgcccggt ccagggggag gtgccaagcc cgatgaaccc gccgtccggc tgcgtgttcc
     1981 atccgcgctg cccgatcgcg gtggaaagct gcaaacacac gcgaccggaa ccgcgggaag
     2041 taaggccggg ccattcggtc gcctgcagcc aggttcactg agagcctgaa aatctttcag
     2101 cgatgcgcca aaaaccgact gtaatgtgcg gcttgggtgg ttagaatcgg cctgtctggc
     2161 tgctgacaaa ggtagggtga tggcatcggg tgacgaggat ggcttgtttg c
//
LOCUS       DASWQN010000492         6691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_838815, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000492 DASWQN010000000
VERSION     DASWQN010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838815"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1050)
                     /gene="map"
                     /locus_tag="VGC82_08110"
     CDS             complement(244..1050)
                     /gene="map"
                     /locus_tag="VGC82_08110"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY0423270.1"
                     /translation="MDGGADSRRITIHAPNDFAGMRAAGRLAAETLDMIVPHVKPGAS
                     TGLLDKLCHDFILDHGATPAPLNYRGYPKSICTSINHVVCHGIPSDRKLENGDILNID
                     VTVILEGWHGDSSRMYAAGTPNTRARNLIDVTYESLMRGLKVVKPGATLGDVGHAIQT
                     YVEGNRFSVVRDFCGHGIGRRFHEPPNVLHFGRLGEGPILRTGMFFTIEPMVNAGRPE
                     VKVLDDGWTAVTRDRSLSAQFEHMIGVTDQGCEIFTLSPAGLDKPPLGVN"
     gene            1228..2166
                     /locus_tag="VGC82_08115"
     CDS             1228..2166
                     /locus_tag="VGC82_08115"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY0423271.1"
                     /translation="MASMIIDMHTHAGRPRRTGDVDKAVLATMRSAGVAAAVVSAIGD
                     IPMIRRNKETGRLEKFRDPDPGECLEAVEGYLASFERSGTRIAREPDEIRVGDPALVL
                     AIEGCDFLESDLDRLDVMAARGVRSIQLTHYLVNETGDIQTAPPVHGGLTPFGADVVR
                     RMNQLGIILDVAHCSRDTVRGVADVATRPFICSHSNLKEPGHKNGDHPRFIDADYARM
                     VVDSGGVVGAWISVLVEEKLPGMIRHMFRLIDALGIDHVGIGSDLPAGVAATEMPDFS
                     RHPELAAALRDRGLNEEEIMKVCSGNWLRVWQAVRA"
     gene            2163..2870
                     /gene="rpiA"
                     /locus_tag="VGC82_08120"
     CDS             2163..2870
                     /gene="rpiA"
                     /locus_tag="VGC82_08120"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008850551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY0423272.1"
                     /translation="MTDQRDAGKQQAALAAVAMVTEGMVVGLGTGSTAAFAIDGLIAR
                     VRDGLHIVGIPTSERSAKQARDGGIELTDFQHHRRIDLTIDGADEIAEGSLDLIKGLG
                     GALLREKIVAAASDRLVIIADGPKLVASLGGRVPVPVEVVPFGWETTAARLANLGSRP
                     VLRTAADGAVFRTDGNNLILDCHFGEIADPARLEQALSNTVGVIETGLFIGMAATVLV
                     ATPEGIHRFDRRVDQEG"
     gene            3082..4248
                     /locus_tag="VGC82_08125"
     CDS             3082..4248
                     /locus_tag="VGC82_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0423273.1"
                     /translation="MTNSWLPAHCGAVSILLASLFATAAGPALAQGGPPPPKVVVAKP
                     IAKQIPRWDEFTGRFEPMEQVEVRPRVSGFIDQIHFKDGQMVQKDDVLFVIDPRPYQI
                     AFDSAKADVMKAKAQTVFAVADYERGEQLVASRTVPVKELEQRRANLDTATASAQSAD
                     AALRNADLNLQWTQVRAPLGGRISDRKVDVGNLVAGGQGTTTTLLTTIVRLDPIYFVF
                     DASEADYLRYARLALTGGRASSRDAPTPVQVNLADETDWPHRGVMNFVDNRLASRSGT
                     IRGRAVLENKDLFLTPGTFGRARLFGGDINALLIPDQAISSDQASKVVMTVGPDNKIV
                     PKPVVLGPIAEGLRVIMSGLTASDQVVISGIANPFVKPGAVVDPTPGEIKTAAK"
     gene            4251..>6691
                     /locus_tag="VGC82_08130"
     CDS             4251..>6691
                     /locus_tag="VGC82_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0423274.1"
                     /translation="MRLSHFFIDRPIFATVLSLLLTIAGAIVQRTLPVSEYPDIAPPT
                     IAITATYPGASAEVISQTVATPIEQEVNGVDDMLYIVSQATGDGRLQINVVFKPGTNI
                     DTAQVLVQNRVAVAQPRLPIDVTRLGVTVKKSSPDLMMVIHMISPDGSRNQQYISNYA
                     TLYVKDVLSRVDGVGDAQVFGARDYAMRIWLDPAKLAARELTVGDVVAALQANNLQVA
                     AGAINQPPAESPGGFQVAVQTLGRLSDPAEFGNIVVRTEDVGGIVRIRDIGRVELGSQ
                     DYTINAYLDDATATALVLFQRPGSNALATSDAVKKVMEEAKRDFPSGLDYSIVYNPTE
                     FVGQSVREVIRTLFEAVGLVVIVVIVFLQTWRAAIIPVVAIPVSLIGCFLVMGAAGLT
                     FNTLSLFGLVLAIGIVVDDAIVVVENVERYLQLGMTPKEAAHKTMDEVGSALIAIALV
                     LCAVFLPTTFITGLQGSFYKQFAITIASATVISAFVSLTLSPALAALLLRPHGHEVKH
                     GVMYRLAAPMRWFFAGFNWVFDRLSRGYAGLTHRLVRMGVIVLLVYAGLIYVAYDRLA
                     KTPTGLVPQLDRGYLIAAFQLPPGSSLTRTDAVVRRAADIIVKRRGVQHSVAFAGFDG
                     GTFTNAPNTGVIFVALKPADERAAQGLSAARIQGDLYQALAPLTDSFTFVLEPPSVPG
                     IGTGGGLKGYVQDRASRGLPALEGAAWTIAGSAMQTPGFAQAFTLFNTHTPQLYTDID
                     RTKAELLGVPISRVFEALSGYMGSAYINDFNLLGRTYRVTAQADEPYRLTPRDVANLK
                     TRNVNGDMVPIGSVAT"
BASE COUNT         1145 a         2127 c         2192 g         1227 t
ORIGIN      
        1 tgtcgggctg ggcgctatag gtgccctgac gctggttttt tcgatccggt ggctgggaag
       61 cgaaggcgct gacgcaagcc tgcgctcaat cgtgctgcca gggcaatctc agcctgactc
      121 taccggccgc caatgctcaa gcgttttgtc tggtcgcatg atcgacatgc ttccaacgtt
      181 ccgcccagtg ctcccgcggg ttacccgcta cggcgcttca tagcggcatg agccggtggc
      241 ctatcagttg acgcccaagg gtggcttgtc caaacccgcg ggagacagcg taaaaatttc
      301 gcagccttgg tcggtgacgc cgatcatgtg ttcgaactgg gcggacaggc tgcggtcgcg
      361 ggtcacggcg gtccaaccgt cgtccagcac cttcacctct ggccggccgg cgttcaccat
      421 cggttcgatc gtgaaaaaca tgcccgtgcg caggatcggg ccttcgccca agcgtccgaa
      481 atgcagcacg ttcggcggct cgtgaaagcg gcggccaatc ccgtggccac agaaatcccg
      541 caccacgctg aagcgattgc cctcgacata ggtctggatg gcgtggccga catcgcccag
      601 cgtcgccccc ggcttgacca ccttcaggcc ccgcatcaac gactcatagg tgacgtcgat
      661 caggttgcgc gctcgcgtat tgggcgttcc ggccgcgtac atgcgtgacg agtcaccgtg
      721 ccagccttcc aggataacgg tcacgtcgat gttcaggatg tcgccgttct caagcttccg
      781 atcgctgggg atgccatggc agacgacatg attgatcgat gtgcagatcg atttcgggta
      841 gccgcgatag ttcagcgggg caggggtcgc accatgatcc aggatgaagt cgtggcacaa
      901 tttgtcgagc agcccggtgg acgccccggg cttcacatgc ggcacgatca tatcgagggt
      961 ctcggccgca aggcggcccg cggcgcgcat tccagcgaag tcgttcggcg cgtggatcgt
     1021 gatgcgcctt gagtctgctc cgccgtccat aagcttcttc cgccttggtc tctcgtgatg
     1081 ccgacaactg actcgccgcg ccggatcggg ccgggcacaa tcagcgtagt caacgtacaa
     1141 catgcgcaac cggctcgtct ggtgcaagac ccacgttcga cgcccggctt caacgcggcc
     1201 gtgacgaacc ggttcggaaa ggtgtaaatg gcgtctatga tcatcgacat gcacacccat
     1261 gccgggcgtc cccgccggac cggcgacgtg gacaaggccg tgctcgcgac gatgcgttcg
     1321 gccggggtgg cggcggccgt ggtatccgcg atcggcgata ttccgatgat ccgccgcaac
     1381 aaggagaccg gccggctgga aaaattccgc gatcccgatc ccggggaatg cctggaagcg
     1441 gttgaaggct acttggccag cttcgaacgg tctggcaccc gcatcgcgcg cgagccggac
     1501 gaaatccgcg tcggcgaccc ggcgttggtg ttggcaatcg aggggtgcga cttcctggaa
     1561 agcgatctcg accggctgga cgtcatggca gcgcgcggcg tgcgcagcat ccagcttacg
     1621 cactacctgg taaacgaaac cggcgacatc cagactgcgc cgcctgtcca tggcggcctg
     1681 acgccgttcg gcgccgacgt ggtgcggcgg atgaaccagt tggggattat cctcgatgtc
     1741 gcccattgct cgcgggatac cgtcaggggc gtcgcggacg tggcgacacg gccatttatc
     1801 tgctcacatt cgaatctgaa ggagccgggc cacaaaaacg gtgatcatcc acgatttatc
     1861 gatgcggatt atgcccgcat ggtggtggac tcgggcggtg tcgtcggcgc ctggatctcc
     1921 gtgctggtcg aagaaaagct tcccggcatg atccgccaca tgttccgctt gatcgatgcc
     1981 ctcgggatcg accatgtcgg cattggatcg gatctgccgg ccggagtcgc ggcgacggaa
     2041 atgccggatt tttctcgcca ccctgaactc gctgccgctc ttcgcgaccg cggcctgaac
     2101 gaggaagaaa tcatgaaggt atgctctggg aactggcttc gcgtgtggca ggcggtgcgg
     2161 gcttgaccga ccagcgtgac gccgggaagc agcaggccgc gctcgccgcg gttgcgatgg
     2221 tcaccgaagg gatggtcgtc gggctaggca ccgggtccac cgcggccttc gctatcgacg
     2281 ggctgattgc ccgcgttcgc gacggcctgc acatcgtcgg catcccaacc agcgaacgca
     2341 gcgccaagca ggcgcgcgac ggcgggattg aactgacgga cttccaacat catcgcagga
     2401 tcgatctgac gatcgacggc gccgacgaga tcgccgaggg ctcgctggac ctgatcaagg
     2461 gcctcggcgg ggcgttgctg cgggaaaaga tcgtcgccgc cgccagcgac cggctggtga
     2521 tcatcgcgga cgggccaaag ctggtcgcca gcctgggcgg ccgggtccct gtcccggtgg
     2581 aagttgtccc gttcggctgg gaaaccaccg ccgctcggct cgccaacctt ggctcgcgcc
     2641 cggttctgcg cacggcggcg gacggcgcgg tcttccgcac cgacggcaac aacctgattc
     2701 tggactgcca cttcggcgaa atcgccgatc cggcccggct tgagcaagcc ttgtccaaca
     2761 ccgtcggggt cattgagacc ggtttgttta tcggcatggc ggccacggtt ctggtggcga
     2821 ctccagaggg aattcatcgc ttcgaccggc gcgtggacca ggaaggctga gcggattgtg
     2881 gatggctcgc gacggtgtgg gtcgagtttt caccttcagg ggcgaaggca gcctgactcg
     2941 aatcttgaag cgaactgccg ccgcggtcgc ccgccccggc cgtttgtccg ggattgaccg
     3001 gacggcatac tccaagctgc gatagggcgt gctgaaactg ctttcgaccg aactgcggaa
     3061 ccgtcaggcc aaggaccacg gatgaccaac tcatggctgc cggcccattg cggtgccgtt
     3121 tctatattac tggcgtcact gttcgctacg gctgcgggac cggcgctcgc acagggcggt
     3181 ccgccgccgc ccaaggtcgt cgtggcgaag ccgatcgcca agcagatccc gcgctgggat
     3241 gagttcaccg gccggttcga accgatggag caggtcgagg tgcggccgcg tgtttctggc
     3301 ttcatcgacc agatccattt caaggacggc cagatggtgc agaaggacga cgtcctgttc
     3361 gtcatcgatc ccaggccgta ccagatcgcc ttcgactcgg ccaaagccga cgtgatgaag
     3421 gccaaggcgc aaacggtgtt cgccgttgcc gactatgagc gcggcgagca actggtagcg
     3481 tcgcggaccg tgccggtgaa agaactggag cagcggcgcg cgaacctcga taccgcgacc
     3541 gccagcgcgc aatccgccga cgcggcactg cgcaacgccg acctcaacct gcaatggacg
     3601 caggttcgtg cgccgctggg ggggcggatt tccgaccgca aggtggacgt cggcaacctt
     3661 gtcgccggcg gccaagggac cacgaccacg ctgctgacca cgatcgtgcg cctcgacccg
     3721 atctatttcg tttttgacgc ctcggaagct gactatctgc gctatgcccg gctcgcgctc
     3781 accggcggcc gagcgtcatc acgcgacgcg cccacgccgg tgcaggtcaa tttggccgat
     3841 gagacggatt ggccgcatcg cggcgtcatg aacttcgtcg ataaccgcct ggcttcccgc
     3901 tccggcacga ttcgcgggcg tgcggtgctc gaaaacaagg accttttcct tacccccggg
     3961 acgttcgggc gcgcacgcct gttcggcggc gacatcaacg cgctgctgat cccggatcag
     4021 gcgatcagct ccgaccaggc cagcaaggtc gtcatgaccg tcgggccgga caacaagatc
     4081 gtgccgaagc cggtggtgct ggggccgatc gccgaggggc tccgcgtcat catgagcggc
     4141 ttgacggcat ccgaccaggt ggtgatctcc ggcatcgcca atccgtttgt caaaccgggg
     4201 gcggtcgtcg atccgacgcc gggcgagatc aagacggcgg cgaagtagtc atgcgcctgt
     4261 cccatttttt catcgaccgg ccgatcttcg cgacggttct gtcgttgctg ctgaccattg
     4321 ccggcgccat cgtgcagcgg acgctgccgg tctcggaata ccccgatatc gcgccgccaa
     4381 cgatcgccat caccgcgacc tatccgggtg cctcggccga ggtgatctca cagaccgtcg
     4441 caacgcccat cgagcaggaa gtcaacggcg tcgatgacat gctgtacatc gtttcccagg
     4501 cgacgggcga cggacggctg cagatcaacg tggtgttcaa gccgggcacc aacatcgata
     4561 ccgcgcaggt gttggtacag aaccgcgtcg ccgtcgccca gccgcggctg ccgatcgacg
     4621 tgacgcggct gggcgtgacc gtgaagaagt cctcgcccga cctgatgatg gtcattcaca
     4681 tgatctcccc ggacggatcg cggaatcagc aatacatctc gaactatgcg acgctgtacg
     4741 tgaaggacgt gctgtcgcgg gtcgatggcg tcggcgacgc acaggtcttc ggcgcacgcg
     4801 actatgcgat gcgcatctgg ctcgacccgg cgaaactggc cgctcgggag ctgaccgtgg
     4861 gcgacgtggt ggcggcgttg caggccaaca acctccaggt cgcggcgggg gccatcaacc
     4921 agccgccggc tgaatccccc ggcggctttc aggtcgcggt gcagaccttg ggccggctga
     4981 gcgatcccgc cgagttcggc aacattgtcg tccgcaccga agatgtcggc ggcatcgtgc
     5041 ggatccgcga catcggccgt gtcgagcttg gttcgcagga ttacaccatc aacgcctacc
     5101 tggacgacgc caccgccacg gcgctcgtac tgttccagcg gcccggatcg aacgcgctgg
     5161 caacctcgga tgccgtcaag aaggtgatgg aggaggccaa acgggatttc ccgagcggcc
     5221 tggactattc gatcgtttat aacccgacgg agttcgtggg ccagtccgtc agggaagtga
     5281 tccgcacctt gttcgaggcg gtcggcctcg tggtcatcgt ggtgatcgtg ttcctgcaga
     5341 cctggcgggc ggcgatcatc cccgtggtgg cgatcccggt atccctgatc ggctgcttcc
     5401 tggtgatggg cgcggcgggc ctgaccttca acacgctgtc gctcttcggc ctggtgttgg
     5461 cgatcggcat tgtcgtcgat gatgcgatcg tggtggtcga gaacgtcgag cgctacctgc
     5521 agttgggcat gacgcccaaa gaggcggcgc ataagacgat ggacgaggtc ggcagcgcgc
     5581 tgatcgccat cgcgctggtg ctgtgcgcgg tgttcctgcc aaccaccttc atcaccggct
     5641 tgcagggatc cttttacaag cagttcgcga tcaccatcgc cagcgcgacg gtcatctcgg
     5701 ccttcgtgtc gctgacgtta tcgccggcgc tggcggcact gttgctgcgg ccacacggtc
     5761 acgaggtcaa gcacggggtc atgtatcggc tggcggcccc gatgcgctgg ttcttcgccg
     5821 gcttcaactg ggtcttcgac aggctgtcgc ggggctatgc cgggttgaca caccggctgg
     5881 tgcggatggg tgtgatcgtc ctgctggttt atgcggggtt gatctatgtc gcctatgacc
     5941 ggttggcaaa gacccccacc gggctggtgc cgcaactcga ccggggctac ctgatcgccg
     6001 cgttccagct tccgcccggc tcctccctga cccgcaccga tgcggtggtg cggcgggcgg
     6061 cggacatcat cgtcaaacgc cggggcgttc agcattcggt tgccttcgcc ggcttcgatg
     6121 gcggcacgtt caccaacgcg cccaacaccg gggtgatctt cgtcgcgctc aagccggcgg
     6181 atgagcgggc ggcgcaaggc ctcagcgcgg cgcggatcca gggcgacctg tatcaggcat
     6241 tggcgccgtt gaccgacagt ttcaccttcg tgctggagcc gccatcggtg cccggtatcg
     6301 gcaccggcgg cggccttaag ggctatgtgc aggaccgggc atcgcgtggc cttccggcac
     6361 tggagggtgc ggcctggacc attgcgggca gtgcgatgca gacgcctggt ttcgcgcagg
     6421 ccttcacctt gttcaacacc cacacccctc agctttacac tgatatcgac cgcaccaagg
     6481 ccgaactgct gggggtgccg atcagccggg tgttcgaagc gctgtccggc tacatgggtt
     6541 cggcctacat caacgacttc aacctgctcg gccggaccta ccgggtcacc gcacaggccg
     6601 acgaaccgta ccggctgacg ccgcgggacg tcgccaacct gaagacccgg aatgtcaacg
     6661 gcgacatggt gccgattggg tcggtagcga c
//
LOCUS       DASWQN010000493         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1497820, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000493 DASWQN010000000
VERSION     DASWQN010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497820"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..459
                     /locus_tag="VGC82_08135"
     CDS             70..459
                     /locus_tag="VGC82_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0423275.1"
                     /translation="MVVDTSALVAILFREPDAEHFGQALAVAPIRLLSAVTRVELSFV
                     IEGRKHDAGRADLELLLREGAFEVVSVTPLQAQIAVDAFRRFGKRRHRAGLNIGDCFS
                     YALAVAMNYPLLFKGNDFAETDIRSAA"
     gene            515..1756
                     /locus_tag="VGC82_08140"
     CDS             515..1756
                     /locus_tag="VGC82_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0423276.1"
                     /translation="MSATQDPLNQPHPRIAANVMHFARLLRRAGLPVGPAETIAAQDA
                     LTRIDLASKIQARTALRTAMIHRHEHREVFDQAFALFWRDPSAAEQAAAMALLESQKQ
                     AKPERAPPASRRVAEAFAAKRDRPPQPKDEPPVTDMTMTVSEQERLQQMDFEAMGAAE
                     IAQAKREIRRLVLPLDLRRTRRLRPDQNGPVTDLRRTIRTSLRQGGEILTIARNRRIT
                     RPPPLVVLCDISGSMARYAQILLHFLHAVTNDRDRVHVFLFGTRLSNVTRQLRARDPE
                     VAFQMVAHAVPDWSGGTRIGEAVASFNRLWAKRVLGQGAVVLLITDGLDRDGAHGLPE
                     NMDRLHRSCTRLIWLNPLLRWSGFEPKSQGIKAMLPHVDEFRPVHNLTSLRSLIDLLS
                     RPAPLAARPPSCFTSMKGSTS"
     gene            1753..>2064
                     /locus_tag="VGC82_08145"
     CDS             1753..>2064
                     /locus_tag="VGC82_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY0423277.1"
                     /translation="MSSTEDVLGTAAAWRAAGEQVAVATVTETWGSSPRPAGSQMAVT
                     KSGRMAGSVSGGCIEGAVAEAAMKTIDSGSPQLLDFGVTNERAWEVGLACGGKVKVFV
                     EK"
BASE COUNT          375 a          728 c          596 g          365 t
ORIGIN      
        1 cttacctgat tatgacacac gcagtccgga cgagatcgtc ggttacgacg aaacaggcat
       61 gtggacctga tggttgtcga tacttcggca ctggtcgcga tcctttttcg cgaacctgat
      121 gccgaacatt ttggtcaggc actagccgtg gcacccattc gattgctgtc ggcggttacc
      181 cgggtcgaac tgtccttcgt catcgaaggc cgcaaacacg atgcagggcg agccgacctg
      241 gaactactgc ttcgcgaggg tgcgttcgag gtggtgtcgg tcacaccgct tcaggctcag
      301 atcgccgtcg atgcctttcg ccgtttcggc aagcgccgac accgggctgg cctcaatatc
      361 ggcgactgct tctcctatgc cctcgccgtc gcgatgaatt atccgctgct gttcaagggc
      421 aatgattttg ctgagacgga catccgatcg gctgcctgac cccgttccgt cctacccctt
      481 gcacgactga ccgacctgtc ttccattagc tccgatgtcc gccacccaag atccgctcaa
      541 ccagccgcac ccgcgtatcg cggccaacgt catgcacttc gcgcgcctgc tccgccgcgc
      601 cggcctgccc gtcggtccgg cggaaacaat cgccgcgcag gacgcgctaa cacgcatcga
      661 cctcgcctcc aaaatccagg ctcgtacggc cttgcgcacc gccatgatcc accgccacga
      721 acaccgcgag gtcttcgacc aggccttcgc cctgttctgg cgtgatccct cggcagccga
      781 acaggcggcc gcgatggcac tactggaatc gcagaaacaa gccaaaccgg aacgcgcccc
      841 gccagcctcg cgccgagtcg ccgaggcctt cgccgccaaa cgcgaccgtc cgccccagcc
      901 gaaggacgaa cccccggtca ccgacatgac gatgaccgtc tcggaacagg agcggcttca
      961 gcaaatggat ttcgaagcga tgggcgccgc cgaaatcgcc caggcgaaac gcgaaatccg
     1021 ccgcctggtc ctgccgctgg acctgcgccg tacccgccgg ctccggccgg accagaatgg
     1081 tcctgtcacc gatctacgtc gaaccatccg gaccagcctg cgccagggcg gtgaaatcct
     1141 gaccattgcc cgcaaccgcc ggatcacccg tccgccgccg ctggtcgtgc tatgcgacat
     1201 ctccggctcg atggcacgct acgcccagat cctgctgcac ttcctccatg ccgtcaccaa
     1261 cgatcgagac cgcgtccacg tctttctctt cggcacgagg ctgtccaacg tcacccgcca
     1321 attgcgcgcc cgcgacccgg aggttgcatt ccaaatggtc gcccacgcgg tgcccgactg
     1381 gtccggcggc actcgcatcg gcgaggccgt cgccagcttc aaccgtcttt gggccaagcg
     1441 cgtgctcggt cagggcgcgg tcgtcctgct gatcaccgac ggcctcgatc gggacggcgc
     1501 ccatggcctg ccggagaaca tggaccgcct gcatcgctcc tgcacaaggc tgatatggtt
     1561 gaacccactg ttgcgctgga gcgggttcga gccgaaatca cagggcataa aggcgatgct
     1621 gccgcatgtg gatgaattcc gcccggtgca taacctgacc agcctgcgca gtctgatcga
     1681 cctgttgtcc cgaccggcgc ctctcgccgc acgccccccc tcttgcttca cgtctatgaa
     1741 gggaagcaca tcatgagcag tactgaagat gtccttggaa ccgcagccgc ctggcgcgcc
     1801 gccggggagc aggtcgccgt cgccaccgtg acggaaacct ggggcagttc ccctcgcccg
     1861 gccggcagcc agatggcggt caccaaatcc ggccgcatgg caggctccgt ctctggcggc
     1921 tgtatcgaag gcgcagtcgc cgaggccgcc atgaagacga tcgacagcgg cagcccgcaa
     1981 ctattggatt ttggcgttac caacgaacgt gcctgggaag tcggcctggc ctgcggcggc
     2041 aaagtgaagg tgtttgtcga gaaa
//
LOCUS       DASWQN010000494         3761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2290007, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000494 DASWQN010000000
VERSION     DASWQN010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2290007"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..901
                     /locus_tag="VGC82_08150"
     CDS             23..901
                     /locus_tag="VGC82_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0423278.1"
                     /translation="MTLDPGTTNGDSVGKRPRVSHLLAIALVLLFAHSAAAQAPGLGA
                     SVELVDPKVFRVCADPHNLPLSDEQGAGFENKIAELFAAKLDEPATYTYFPQVIGFLR
                     NTLNALRCDVVIGTAVGDPMVQTTSAYYRTTYALLFKPNSGLDGVDSLGDPRLKDKHI
                     GIVAGTPPATILTRNGLMALAKPYPLTVDTRATSSPEMMTNDIADGTIDAGVLWGPMA
                     GYYAKHASPHLVVVPLLKEGQQMDFRIAMGVRRTDQDWKRKLNRLIAENQPEIDKILT
                     DYGVPLLDEQGQLKAP"
     gene            898..1452
                     /locus_tag="VGC82_08155"
     CDS             898..1452
                     /locus_tag="VGC82_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated CXXCW motif
                     protein"
                     /protein_id="HEY0423279.1"
                     /translation="MKALHLILVLCVSFTAKAAEPVPEPQGYRMEAYRAPTPQTLQGA
                     TVLTTDAARDLWSRQSAAFIDVLPRPPRPAGLPASTIWRPKPRSDIPGSIWLPDTGYG
                     ALAPVMEQYFERGLSEAGGGDHGRMLVFYCQTDCWMSWNAAKRAIALGYTHVGWYPDG
                     TDGWEAQGLPTESREPVAQPPATE"
     gene            complement(1453..2061)
                     /locus_tag="VGC82_08160"
     CDS             complement(1453..2061)
                     /locus_tag="VGC82_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423280.1"
                     /translation="MIRYVLVAALLAPVVAFADPPGGRDSNWPCQQIKVPELSLAAVW
                     SGPTVDLEQTGWKDDPEVADLVQKLVPRREPIDQAQSLIHDFAQHAGERKQAQLLQLL
                     VGLFTDLGEERESVMSGLDRFGGRQKELATQVREDNEKLRAMQTGAASDTPAIQQMTQ
                     KVTWEAEVFQDRRQALTYACDVPGKIEQRLFSLARLIQQELE"
     gene            complement(2058..2891)
                     /locus_tag="VGC82_08165"
     CDS             complement(2058..2891)
                     /locus_tag="VGC82_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0423281.1"
                     /translation="MSASAIGSGFTAGQYFICLRGIVWRETLRFIHQRGRFISAIVRP
                     LVWLFIFAAGFRQILGVSIIPPYDTYVLYDVYVTPGLVAMIQLFNGMQSSLSMVYDRE
                     MGNMRMLLVSPFPRWFLLMGKLLASTSVSLLQVFAYLVIAWLWGAQAPGWGYVAVIPA
                     LVLSGMMMGSLGMLLSSGIKQLENFAGVMNFVIFPMFFASSALYPLWLVQQSSPAVYF
                     ICRLNPFTYAVELIRFALYLEVDWISMAVVAGATILFTLGAITAYDPARGLVSRRGGP
                     G"
     gene            complement(2888..>3761)
                     /locus_tag="VGC82_08170"
     CDS             complement(2888..>3761)
                     /locus_tag="VGC82_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0423282.1"
                     /translation="HRCRIVKRREVDPGRTPTLGRCRGTQMSTAIAGDAATSLRNTPG
                     IPALAVTGVSHAYGARKALDDVNLTIPQSRFTALLGLNGAGKTTLFSLITRLYDTQKG
                     SIDVLGYRVSREPGEALRRLGVVFQARTLDLDLSVMDNLLYHAALHGIGGREARRRAE
                     AALTSVELLERARDPARLLSGGQARRVEIARALLHDPSVLVLDEATVGLDVNSKSAIV
                     RHVRSLVRDRGVAVLWATHLLDEISADDAVVVLHRGRVVASGDVTAVVEAAGTADMRS
                     AFIALTGGGKDETE"
BASE COUNT          787 a         1210 c         1115 g          649 t
ORIGIN      
        1 aggccgagaa tgaatgcatg ggatgacgct ggatcctgga accacgaatg gcgattccgt
       61 cggcaagcgc ccacgcgtat cgcaccttct ggccattgct cttgtcctgc tcttcgccca
      121 ttccgcagct gcgcaagccc cgggactcgg tgcgtcggtg gagttggtcg atcccaaggt
      181 gttcagggtg tgcgccgatc cacacaatct tcctctgtca gacgagcagg gtgcgggttt
      241 cgagaacaag atcgctgagt tgttcgcagc gaaactggac gaacccgcta cctacacgta
      301 cttcccacag gtgatcggct tcctgcgcaa taccctgaac gcactgcgct gcgatgtcgt
      361 catcggcact gccgtcggcg atcccatggt gcagaccacg agtgcctatt accgcacgac
      421 ctacgcactt ctgttcaagc cgaacagcgg cctggatggc gtggactcgc ttggcgaccc
      481 acgcctgaag gacaagcaca tcggcatcgt tgccgggaca ccccctgcta ccatcctgac
      541 gcggaatggc ttgatggctt tggccaaacc gtatccgctg accgtagata cgcgtgccac
      601 ctcgtcaccg gagatgatga cgaacgacat tgccgatggc acgatcgacg cgggcgtctt
      661 gtggggtccc atggccggct actacgcgaa gcatgcctcc ccgcatctgg tcgtcgttcc
      721 cctgctgaag gagggacagc aaatggattt ccgcattgcc atgggggtgc gtagaaccga
      781 ccaggactgg aagcgcaagc tcaacaggct gatcgcggaa aatcagccgg agatcgacaa
      841 gatcctgacg gactacggcg tccccctgct ggacgagcaa ggtcagctca aggcgccatg
      901 aaagcgctgc acctgattct ggtgctttgt gtatccttca cggcgaaagc ggccgaacca
      961 gtgcccgaac cacaaggtta tcgtatggag gcgtaccgcg cgccgacacc gcagacgctg
     1021 caaggcgcca cagtcctgac cactgatgcg gcgcgcgact tgtggtccag acagtctgcg
     1081 gctttcatcg acgtgctgcc gcgcccgccg cgccccgccg ggctgccggc atctaccatt
     1141 tggcggccga agccacgctc cgacatcccc ggcagtatct ggttgccaga caccggatac
     1201 ggcgcattgg ccccggtcat ggaacaatac ttcgaacgtg gcctgtcgga agccgggggc
     1261 ggcgaccatg gccgcatgct ggtgttctac tgccagacgg attgctggat gtcatggaac
     1321 gcggcgaaac gtgccattgc actcggctat acacatgtcg gctggtatcc cgacggtacc
     1381 gacggctggg aagcacaggg gctgccaacc gaatcgaggg agcccgtggc acagccgccg
     1441 gccacggagt gatcattcca gttcttgctg gatcagccgc gccagcgaga acaaccgttg
     1501 ttcgatcttg cccggtacat cgcaggcata ggtcagggcc tgccgccggt cctgaaagac
     1561 ctccgcttcc catgttactt tttgggtcat ttgctggatc gccggcgtat cggacgccgc
     1621 gccagtctgc atcgcgcgca gtttttcgtt atcctcccgg acctgcgtag cgagctcttt
     1681 ctgccggccg ccaaagcggt ccaggccgct catgacgctt tcgcgttcct cgccaagatc
     1741 ggtaaacagc ccaacaagca attgcaggag ttgcgcctgt ttccgctcgc cggcgtgctg
     1801 cgcgaaatcg tgaatcaagg attgtgcctg atcgatcggc tcccgccgcg gcacaagttt
     1861 ttgcaccaga tcggcgacct cgggatcgtc tttccagccg gtctgctcaa gatcgaccgt
     1921 aggccccgac cagaccgccg ccaacgagag ttccggcacc ttgatctgct gacacggcca
     1981 gttggagtca cgtccgccgg gcggatcggc gaacgcgacc accggcgcca gcagcgccgc
     2041 aaccagaaca tagcgaatca tcccggacct ccacggcggg aaacgagacc acgcgccggg
     2101 tcataggcag tgatcgcgcc caaggtgaaa aggatcgtcg ccccggccac gacggccatc
     2161 gaaatccaat cgacctccag gtacagggca aagcgaatga gctcgacggc ataggtgaac
     2221 gggttcagcc gacaaatgaa gtaaaccgcc gggctggatt gctgcacgag ccacaacgga
     2281 taaagcgcgg acgacgcgaa gaacatcggg aaaatcacga agttcatcac gccggcgaag
     2341 ttttccaact gcttgatgcc ggacgacaaa agcatgccca gcgagcccat catcattcca
     2401 gaaagcacca gtgcggggat cactgcgaca tagccccaac caggtgcctg cgcaccccat
     2461 agccaggcga tcaccaggta ggcgaacacc tgcagcagcg aaacggaggt gctggccagc
     2521 aattttccca tcagcaggaa ccagcgcgga aaagggctca ccagcaacat gcgcatgttg
     2581 cccatttcgc gatcgtacac catggacaac gaggactgca tgccgttgaa caactggatc
     2641 atcgcaacca gtccaggcgt aacataaacg tcgtacagga cataagtgtc gtacggcgga
     2701 atgatcgata cgccgaggat ttgccggaat ccggcggcga agatgaacaa ccagaccagc
     2761 ggcctgacga tagccgagat gaaacgtccg cgctgatgaa tgaagcgcag cgtctcccgc
     2821 cagacgatgc cgcgcaggca aataaagtac tggcccgcgg taaatccgct gccgatcgcg
     2881 ctggcgctca ttcggtttcg tccttgccgc cgccggtcag ggcgataaac gccgaacgca
     2941 tgtcggcggt gccggcggct tcgacgaccg cggtaacatc gcctgacgca acgacccggc
     3001 cacggtgcag caccacgacc gcatcgtcgg ccgagatctc atccagcaaa tgggttgccc
     3061 acagcaccgc cacgccgcgg tcgcgcacca gcgaccggac gtgccgcacg atggccgatt
     3121 tggaattcac atcaagaccg accgtcgctt cgtcgagcac cagcacgctc gggtcatgca
     3181 gcaatgcccg ggcgatctcc acccggcgcg cctggccgcc ggaaagcaat cgcgccggat
     3241 ctcgcgcgcg ttcgagcagt tcaacgctgg tcagcgccgc ttccgcccgc cgccgtgctt
     3301 cgcgtccgcc gatgccgtgc aacgcggcgt ggtaaagcag gttgtccatc acggaaagat
     3361 cgagatcgag cgtgcgagcc tggaacacca cacccagccg gcgtagtgcc tctcccggtt
     3421 cccgcgaaac acggtagccc agaacgtcga tcgacccctt ctgggtatcg taaagccgtg
     3481 tgatcagcga aaacagcgtt gtcttgccgg caccgttcaa tcccagcaat gccgtgaagc
     3541 gtgattgcgg aatagtcagg ttgacgtcgt ccagtgcctt gcgcgcaccg tatgcgtggc
     3601 tgacgccggt caccgccagt gccggaatgc ccggcgtgtt ccgaaggctg gttgcggcat
     3661 cgccggcaat ggccgtgctc atttgggtgc cacgacaacg ccccagggta ggcgtccgac
     3721 cgggatcgac ttcacgacgc ttaacgatgc gacatcgatg a
//
LOCUS       DASWQN010000495         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2554247, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000495 DASWQN010000000
VERSION     DASWQN010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2554247"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..>1539)
                     /locus_tag="VGC82_08175"
     CDS             complement(185..>1539)
                     /locus_tag="VGC82_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0423283.1"
                     /translation="IEVEYALNKVGCKALITASRFKTSDYIGMINTLAPELAICHPGE
                     LHAKRLPSLRSVIQIGGDDPGTLAFDKIAHHGREVDRRRLKELASLLQFDDPINIQFT
                     SGTTGLPKGATLTHHNILNNGFFIGEVMRYTDQDAVCIPVPMYHCFGMVIGALACMTH
                     GSAIVFPGEGFDPLATLQTVAEYRCTSLYGVPTMFIAELDHPNFSQYDLTSLRTGMMA
                     GAPCPVEVMRRCIRDMHLTEMTIGYGMTETSPVSTQTRIGDSLEKQVATVGRPHPHVE
                     VKIVDTEGRIVPRGVTGEFCTRGYPVMLGYWDEPALTAEAIDRAGWMHTGDLATMDAD
                     GYCAIVGRIKDMVIRGGENIYPREIEEFLYRHPAIIDVQVFGVPDKRFGEELCAWVKL
                     RAGATLTEDDIKAFCKDQIAHYKVPRHIRFVDEFPMTVTGKMQKFLMRATMTEELGSN
                     "
BASE COUNT          292 a          472 c          462 g          313 t
ORIGIN      
        1 gaacgtcggc ggaatcaccg gcaccagcag ctttacgtcc gacagcttca gtttctgact
       61 tgtgcgttcc caggcctcga acggagatcc gcgaaccgga atgacgatat cgccctcgac
      121 caccgcatag gaaggcgagc cgttctgttc gaagcgtgcc cagcgcatga cattttcccc
      181 tgactcagtt tgatcccaat tcttcggtca tcgtggcacg catcaggaat ttctgcatct
      241 tcccggtcac ggtcatggga aactcgtcca cgaaccggat gtggcgcggc actttgtaat
      301 gggcgatctg gtccttgcag aacgctttga tgtcgtcctc ggtcagggtc gccccagcac
      361 gcagcttgac ccaggcgcag agttcctcac cgaagcgctt gtccggtaca ccgaacacct
      421 gcacatcgat gatcgccgga tgacgataga gaaattcttc aatttcacgc ggatagatgt
      481 tctctccgcc acggatcacc atgtccttga tgcgccccac gatcgcgcag tagccatccg
      541 catccatcgt tgcgaggtcg ccggtgtgca tccagccggc gcggtcgatg gcttccgcgg
      601 tgagtgccgg ttcgtcccag taacccagca tgacgggata gccgcgggta cagaattcgc
      661 cggtgacccc gcgcggcaca atccgccctt cggtgtcgac gatcttgacc tcgacatgcg
      721 gatgcggccg gccgacggtc gccacctgct tttccagcga gtcgccgatc cgcgtctggg
      781 tgctgaccgg gctggtctcg gtcatgccgt agccgatggt catctcggtc aggtgcatgt
      841 cgcggataca gcggcgcatg acctcgaccg ggcacggggc gcccgccatc atgccggtgc
      901 gcaggctggt caggtcgtat tgcgaaaaat tcggatggtc gagttcggcg atgaacatcg
      961 tcggcacgcc atacagcgac gtacagcgat attcggcgac tgtctgcaaa gtggcgagcg
     1021 ggtcgaagcc ctcgcccgga aacacgatcg ccgaaccatg ggtcatgcag gccagcgcgc
     1081 cgatcaccat gccgaagcaa tggtacatcg gcaccggaat gcagaccgcg tcctggtcgg
     1141 tgtagcgcat cacctcgcca atgaagaagc cgttgttcag gatattgtgg tgcgtcaacg
     1201 tcgcaccttt cggcagcccg gtcgtgccgg aggtgaactg gatgttgatt ggatcgtcga
     1261 actgcagcag cgaggccagt tccttcaatc gccggcggtc cacttcccgg ccatgatggg
     1321 caatcttgtc gaatgccaag gtcccgggat cgtcgccgcc gatctggatg acagagcgca
     1381 gcgacggcag ccgtttagcg tgcaactcgc ccggatgaca aatagcgagt tccggcgcca
     1441 gcgtgttgat catgccgatg tagtcgctgg tcttgaagcg cgaggcggtg atcaacgctt
     1501 tgcagccgac tttgttcagc gcgtactcga cttcgatca
//
LOCUS       DASWQN010000496         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_47380, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000496 DASWQN010000000
VERSION     DASWQN010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47380"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..463)
                     /locus_tag="VGC82_08180"
     CDS             complement(275..463)
                     /locus_tag="VGC82_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423284.1"
                     /translation="MSNVWQPVSGTRFYSRNGEGLYERLTDQLFLRVDDVDETDAQEV
                     CSRFADAFEARAPVRMVA"
     gene            complement(1076..2122)
                     /locus_tag="VGC82_08185"
     CDS             complement(1076..2122)
                     /locus_tag="VGC82_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEY0423285.1"
                     /translation="MAKFKIATPAGASFTTAGGGYDHEMEPLEGIDAEIVEGPTDEDG
                     FIKFAQDADAIYAKGMRFTKKVVEGLPERCRAIVLGSVGVDSIDIVAATARGMPVTNC
                     PDTFIEEVADHAMMLLLASHRRTLGQDRMVREGRWREGRPALLQIPRLMGQTLGLIAF
                     GHVARAVAKRAKPFGLRVIAYDPFIEELVMVEHGVLPMTLEEVLAQSDFVSMHAPATP
                     EAEGMLMEKHFRQMKKTAVFINTGRGPTVQESALIKALSEGWIAHAGLDVLEIEPPGN
                     NNPILGMPNVTLSAHTASASARFDPARKRHVGRELGLVLSGKWPMSCVNPAVLMKSNL
                     RRWQPVSMERGPNS"
     gene            complement(2212..>3046)
                     /locus_tag="VGC82_08190"
     CDS             complement(2212..>3046)
                     /locus_tag="VGC82_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY0423286.1"
                     /translation="ERIPVYWSHFGTYRVRSAALMGVPELRTYDDLARHAQEVRDKGF
                     RALKTNILPLADGRLSSYVPGFGRTVGWPELNWDNKLTRGVTEQLSVIREAVGPDMGI
                     HLDINFNFKTEGFKRIAEAVAGANLTWLEIDTHDAAALAEIKRSAPCPVASCETLHGR
                     REFKPFFDHYATDVAIVDVIWNGLLESAKIASMADVYEVNVAPHNFYGHLCSAISATF
                     SAIVPNFRVMEIDIDSAPWRDEFYTVVPTIENGEFILPPGPGWGIDINEKAVRARPPK
                     N"
BASE COUNT          604 a          956 c          873 g          613 t
ORIGIN      
        1 ggagggtacg aagcgcctga tgcgcatccc ggaagaagct ggctacggtg gcagcgaatt
       61 cgtcatcgac tactcgccgg cgtaacaggc acgaccgatt ttgctaccgg ataatttgct
      121 accggataaa tggaaagacc agcggttcgt cagccacaag cggtgtcaaa tttcttgcgc
      181 tgacctcaca ccgggaaaga cgttcacacc cccgccttgg ccacaccgac agtttgaaaa
      241 cagtttgaaa agaaaatcgg cgaccggatg tcggtcaggc aaccatccgg accggggcac
      301 gggcttcgaa agcgtcggca aaacgactgc agacttcctg cgcgtcggtt tcgtcaacgt
      361 cgtccacacg aaggaacagc tggtcggtca ggcgctcata gagaccctcg ccgttgcgcg
      421 aataaaaacg cgtgccggac acgggctgcc atacgttgct catgatcgat ccctcctgat
      481 cgtcgcccct gcggtcaagg ccatcgttgg gcggagaggc gttccgcttc ggcgaagcgt
      541 ttcggacaaa gatgagttgt tgtcaatatc ggttcgatcg aacaagcaca ggatacttgc
      601 ctgacgggag tgtaaccaac ttcaaaatct cgtgaacgcg aagccggcgg cgctgcaaca
      661 tgaaatctcc tacgacgaac gtcgttctga tcccaccgaa tacaaagatc gtcgccgaca
      721 agagggaacc cggcgcgaaa tacatttaaa acggctcatt tcacccagtt tcacttcgat
      781 gacacggtga atctgcgtat ttcgcgacat ggccgttcgg cggattgtcc tgctcggttt
      841 cgcgaccatc ggacgcttcc acgccagatg gagccgtccc ggcttacggc tcagccatgc
      901 tggcgacagg ccttaccggt gacaggcgct tttttcgcgc tgccgcttgg accggacccg
      961 atacaagcgc gttgttatcg ccccttgcga tggtagcgat ggcgataatg atagtggatg
     1021 gcgggcttcc aggggaaacg gaaaggcaga acctttccac cccccttccc ttagcctagc
     1081 tgtttggccc tcgttccatc gataccggct gccaccggcg caggttgctc ttcatcagca
     1141 ccgctgggtt gacgcaactc attggccact tgccgctcag gaccaaccct agttcccgcc
     1201 cgacatgccg cttgcgcgca ggatcgaacc gagcggacgc cgatgcagta tgggccgata
     1261 gcgtcacatt cggcattccc aggataggat tgttgttccc cggcggctcg atttccagca
     1321 cgtccaagcc ggcatgggca atccagcctt ccgacagcgc cttgatcaac gcagactctt
     1381 gcaccgtcgg tccgcggccg gtgttgatga aaacagctgt cttcttcatc tggcggaaat
     1441 gcttttccat caacattccc tcggcctccg gcgtcgccgg cgcgtgcatg gaaacgaagt
     1501 cggactgcgc cagcacctcc tccagcgtca tcggcagcac gccgtgctcc accatgacca
     1561 gttcctcgat gaacggatca taggcgatca cgcgcaatcc aaacggcttg gcgcgtttcg
     1621 ccacggcgcg ggcgacatgg ccgaacgcga taaggccgag ggtctgaccc atcaggcgcg
     1681 ggatctgcag cagcgccggg cggccttccc gccagcggcc ttcgcggacc atccggtcct
     1741 gccccagcgt ccgccggtgc gaggccagca gcagcatcat cgcgtggtcg gcgacctcct
     1801 cgatgaaggt gtcggggcag ttcgtcaccg gcataccgcg ggcggtggct gcgacgatgt
     1861 cgatgctatc cacgccgaca ctgcccagca cgatggcacg gcaccgctcg ggcagccctt
     1921 cgacgacttt tttggtgaag cgcatgccct tggcatagat cgcgtccgcg tcctgggcga
     1981 attttatgaa cccatcttca tcggtgggtc cttcgacgat ttcggcgtcg atgccctcca
     2041 gcggttccat ctcgtggtca taaccgccgc cggcggtggt gaagctagcc cctgccgggg
     2101 ttgcgatctt gaactttgcc aattttaaac tcctcctttg aatgcggaac gatccgggcg
     2161 ctcatggcag cgccgtcccg gggccaaggc cgatgcgtct tgtggtcgct ttcagttctt
     2221 cggcggccgt gcccggacgg ctttttcgtt tatgtcgatc ccccatccgg gacctggcgg
     2281 caggatgaat tcgccgttct cgattgtcgg cacaaccgta tagaattcgt cgcgccaagg
     2341 agcgctgtcg atgtcgatct ccatgacccg gaaattcggc acgatcgcgc tgaacgtcgc
     2401 gctgatggca ctgcacaaat ggccatagaa gttatgcggg gcgacattga cctcatacac
     2461 atcggccatc gaggcgatct tcgctgattc cagcagcccg ttccagatga cgtcgacgat
     2521 cgccacatcg gtcgcgtagt ggtcgaagaa cggcttgaat tcgcgcctgc cgtgcagcgt
     2581 ttcgcaagac gcgaccggac acggcgcgga ccgcttgatc tccgccaacg ccgctgcatc
     2641 gtgggtatcg atctccagcc aggtcaaatt cgccccggca accgcctcgg cgatacgctt
     2701 gaagccctcg gtcttgaagt tgaaattgat atcgaggtgg atccccatgt ccggaccaac
     2761 cgcctcgcgg atgaccgaca attgttccgt cacgcctcgt gtcagcttgt tgtcccagtt
     2821 cagttccggc cagccaacgg tccgaccaaa ccccgggacg taggaggaca gccgcccatc
     2881 ggccaacggc aggatattcg tcttcagtgc gcgaaaacct ttatcgcgaa cctcctgagc
     2941 atgccgggca aggtcgtcat aggtgcgcaa ttccggaacg cccatcaggg cggcgctgcg
     3001 cacgcggtat gttccaaaat gtgaccagta aaccgggata cgctcg
//
LOCUS       DASWQN010000497         5985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_970255, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000497 DASWQN010000000
VERSION     DASWQN010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970255"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VGC82_08195"
     CDS             <1..602
                     /locus_tag="VGC82_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0423287.1"
                     /translation="YEQIRAPFDGVVTARFVDVGALVQNSTTNKTSNQPVLTVADDSK
                     LRVDVFVEQRDVPFVHVGDLAEIADGANSARTIQARIARTSNELDPRTRTLFVELDVD
                     NADRFLVPGSFAYVTLHIPMRSYPEIPVAGLIVRGTRTMIADLDSDQIVHLRPVTVAT
                     TDGIRAMLSEGATVGQKVAINLPDEVNDGGRVQPVAGGR"
     gene            897..1850
                     /gene="mdh"
                     /locus_tag="VGC82_08200"
     CDS             897..1850
                     /gene="mdh"
                     /locus_tag="VGC82_08200"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY0423288.1"
                     /translation="MARNKIALIGAGNIGGTLAHLIGLKELGDVVLFDVFGGVAAGKA
                     LDIMQSGPVEGFDALMTGGSDYAAIKGADVVIVTAGFPRTPGMSRDDLIGKNAGVIAQ
                     VAEGIKTNCPDAFVICITNPLDAMVWVMQQKSGLPTNKVVGMAGVLDSSRFQLFLAQE
                     FGVSVQDVTAFVLGGHGDTMVPLTRYSTVAGIPVPDLIKMGWSTQAKIDAIVERTANG
                     GGEIVKLLERGSAFYAPAASAIAIAEAYLKDKKRVLPCAAYLTGQYGIKDMYVGVPVV
                     IGAGGVEKIVEIELNATEQAAFDKSCGAVKELIEASRKLIG"
     gene            complement(1847..2011)
                     /locus_tag="VGC82_08205"
     CDS             complement(1847..2011)
                     /locus_tag="VGC82_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423289.1"
                     /translation="MAPQPVFGLAKQPEDRLVRCGIWCALDGTPSTIMLGTGSHDSRA
                     LDGTPSTIML"
     gene            2014..3210
                     /gene="sucC"
                     /locus_tag="VGC82_08210"
     CDS             2014..3210
                     /gene="sucC"
                     /locus_tag="VGC82_08210"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY0423290.1"
                     /translation="MNIHEYQGKELLKRYGVAVLDGYVAWTPDEAEAAAARLPGPVYV
                     VKSQIHAGGRGAGRFADDPNGKGGVRLARSPAEVRAAAEAMIGHTLITKQTGETGRPV
                     RRVYVEAGCDIARELYVSLLVDRITGRVTVVASTEGGMEIEDVAANHPEKILQVGIDP
                     ASGISAFHGRRLAASLGLTGQQAASFGRFVAAMYQAFMAQDCAVIEINPLVVTVAGDI
                     VALDAKVSFDDSALFRHPELEKLRDEAEEDPKELEAARFELNYVALDGNIGCMVNGAG
                     LAMATMDIIKLFGMSPANFLDVGGGATKERVTAAFKIILSDPNVEGILVNIFGGIMRC
                     DVIAEGVVSAARDVQLSVPLVVRLAGTNVDLGKTILARSGLPIVAADNLEDAAEKIVK
                     AVREAA"
     gene            3211..4086
                     /gene="sucD"
                     /locus_tag="VGC82_08215"
     CDS             3211..4086
                     /gene="sucD"
                     /locus_tag="VGC82_08215"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY0423291.1"
                     /translation="MSVLVNAATKVICQGFTGAQGTYHSEQAIAYGTKMVGGVTPGKG
                     GTTHLDLPVFNTVWECREATGADASVIYVPPPFAADAILEAIDAEFPLIICITEGIPV
                     LDMVRVKRALSGSKSRLIGPNCPGVITPDECKIGIMPGHIHRRGTVGIVSRSGTLTYE
                     AVAQTTAAGLGQTSCVGIGGDPVKGTDFIEVLEMFLADPETKQIVMIGEIGGSSETDA
                     AEFLSRNKVKKPTVGFIAGAAAPPGRRMGHAGAVISGGKDTAAAKMDAMRSAGIHVAD
                     SPAALGSTMVEVLKG"
     gene            4379..5521
                     /locus_tag="VGC82_08220"
     CDS             4379..5521
                     /locus_tag="VGC82_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEY0423292.1"
                     /translation="MDKTLHDRLTGWRRYLHAHPELTLHERETAAFVQARLAELGVPF
                     TSGIGGNGVVATISRGQSNRSVGLRADMDALPILETTGLPHASVSPGVMHACGHDGHT
                     TSLLGAVALLSADTGWSGTVHFVFQPAEEGGGGAKSMIADGLFERFPMERIFGYHNWP
                     GLEAGTVAVHDGPVMAAGNRVLFTVTGHAGHAALPHLTRDPMVASAHLLLALQSIVSR
                     TVDPLDSAVVTIGTMQAGSAANQISDRAVMRGTIRTLRNEVAAQVKQAIHRIAEGVGH
                     SFGVSIDVEIPPGNLVTANTPAERDLAAEAVASAGLKLRRDMAPAMTGEDFSWFLQQR
                     PGAFVWIGNGDSAGGRELHNSNYDFNDAILPAAAGYLASVARRALG"
     gene            5589..>5985
                     /locus_tag="VGC82_08225"
     CDS             5589..>5985
                     /locus_tag="VGC82_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEY0423293.1"
                     /translation="MAEIPAQFLDLLTEKKAFAHIATLQPDGSPQVTPVWFDFTNGVV
                     RVNTAKGRVKARNLSEGSKVALSIIDPDNAYRYIQIRGTVTKETTDGAVAHIDSLAKK
                     YLGKDVYPWHSDKETRVIYEITPSATHTMG"
BASE COUNT         1112 a         1852 c         1972 g         1049 t
ORIGIN      
        1 cgtatgagca aatccgcgcc ccgttcgatg gtgtcgtgac ggcccggttc gtcgatgttg
       61 gggcgctggt gcagaattcg acgaccaaca agaccagcaa ccagccggtg ctcacggtcg
      121 cggacgacag caagctgcgg gtcgatgtat tcgtggagca gcgcgacgtg ccgttcgtcc
      181 atgtcggcga cctcgccgag attgccgacg gcgcgaattc cgcgcgaacg atacaggccc
      241 gtattgcccg aacatcgaac gagctggacc cgcgcacccg gaccttgttc gtcgaactgg
      301 atgtcgataa cgcggaccgc tttctggtgc cgggaagctt tgcctacgtg acgctgcata
      361 tccctatgcg cagctatccg gaaattccgg tggcgggact gatcgtgcgc ggcacccgca
      421 cgatgatcgc cgacctcgac agcgaccaga tcgtgcatct gcgtccggtg acggtagcga
      481 cgaccgacgg catccgcgcc atgctgtccg aaggcgcgac agtgggtcag aaagtggcga
      541 tcaatctgcc ggacgaggtc aacgatggcg gacgggtgca accggtagcg ggcggacggt
      601 aggcgccatc gaagtgggcc taatgaggcg ctgaacgtcg atcctccggt ccgtcaagat
      661 agagcacagc gtctcgttac tctggcaact cgtcgcccga tccgggagcc cgaccgccaa
      721 tccgggtcaa gaaccggata gccgcatctg caatgctgca ctgcaccgcc ttgccgggtg
      781 ttctgcgatg gagtagcaag cgccccacct ttcgggcagc catcccggcc aaacgctcgg
      841 caggctccgt tacacaccat ccggcgccgc caaggcgccc tcggaaggac ttgtccatgg
      901 cccgcaacaa gatcgccctg atcggcgccg gtaacatcgg cggaacgctc gcccacctga
      961 tcggcctgaa ggagctagga gacgtcgtgc tgttcgacgt gttcggcggc gtcgcggcag
     1021 gcaaggcctt ggacatcatg cagtccggcc cggttgaggg gttcgacgcg ctgatgacgg
     1081 gcggctcgga ctatgctgcc atcaagggcg ccgacgtggt gatcgttacc gccggtttcc
     1141 cccgcacgcc agggatgtcg cgcgacgatc tgatcggcaa gaacgccggc gtcatcgccc
     1201 aggtcgccga gggcatcaag accaactgcc cggacgcttt cgtcatctgc atcaccaacc
     1261 cgctcgatgc catggtttgg gtgatgcagc agaaatccgg cttgccgacc aacaaggtcg
     1321 tcggcatggc gggtgtgctg gacagctcgc gcttccagtt gttcctggcg caagaattcg
     1381 gcgtgtcggt gcaggacgtg accgccttcg tgctgggtgg gcatggcgac acgatggtgc
     1441 cgctgacgcg ttactcgacc gtcgcgggca tcccagtgcc ggacctgatc aagatgggct
     1501 ggtccacgca ggccaagatc gacgccatcg tcgagcgcac cgccaacggc ggcggcgaaa
     1561 ttgtcaagct gctcgaacgc ggcagcgcct tctacgcacc ggccgcgtcg gcgatcgcca
     1621 tagccgaggc ctacctgaag gacaagaagc gcgtcctgcc ctgtgccgcc tacctgactg
     1681 gtcaatatgg catcaaggac atgtatgtcg gcgttccggt cgtcatcggc gccggcggcg
     1741 tggagaagat cgtcgagatc gaactgaacg ccaccgaaca ggctgccttc gataagtcct
     1801 gcggggcggt gaaggaactg atcgaggctt cgcgcaagct gatcggctag agcatgatcg
     1861 tgctgggcgt accgtccaaa gcgcgtgaat catgcgatcc agtaccgagc atgatcgtgc
     1921 tgggcgtacc gtccaacgcg caccagatcc cacaacggac cagccggtcc tcaggctgct
     1981 tggccagccc gaagaccggc tgaggagcca ttcatgaata tccacgaata tcagggcaag
     2041 gaattgctga agcgctacgg tgtcgcggtg ctggacggct atgtggcgtg gacgccggac
     2101 gaagccgagg ccgccgccgc caggcttcca gggccggttt atgttgtgaa gtcgcagatc
     2161 catgccggcg ggcggggcgc cggtcgcttc gccgacgacc cgaacggcaa gggtggcgtg
     2221 cgtctcgcgc ggtcgccggc cgaggttcgg gccgcggcgg aagcaatgat cggccatacc
     2281 ctgatcacca agcagaccgg tgaaaccggc cggccggtgc gccgggtcta tgtcgaggcg
     2341 ggatgcgata tcgcccgcga actctatgtg tcgttgctgg tggaccggat cactggccgc
     2401 gtgaccgtcg tggcctctac cgaaggcggc atggagatcg aggacgtcgc cgccaaccat
     2461 ccggaaaaaa tcctccaggt cggcatcgat ccggcctccg gcatttccgc cttccatggc
     2521 cgccgcctcg ccgccagcct tggcctgacg ggccagcagg cggcatcctt tggcaggttc
     2581 gttgctgcga tgtatcaggc gttcatggcg caggattgcg cggtgatcga gatcaacccc
     2641 ttggtcgtga cggtcgcagg cgacatcgtg gcgctggatg ccaaggtcag cttcgacgac
     2701 agtgcgctgt tccgccatcc ggagctggaa aaactccgcg acgaggccga agaggatccg
     2761 aaagagctcg aggccgctcg gttcgagctg aattacgtgg cgctggacgg caacatcggc
     2821 tgcatggtca acggggccgg cctggccatg gcgacgatgg acatcatcaa gctgttcggc
     2881 atgtcgccgg cgaacttcct ggatgtcggc ggcggcgcca ccaaggaacg ggtgacggcc
     2941 gcgttcaaga tcatcctgtc ggatccgaac gtcgagggca tcctggtcaa tattttcggt
     3001 ggcatcatgc gctgcgacgt catcgccgag ggtgtggtct cggctgcgcg cgatgtgcag
     3061 ctttcggtgc ccctggtggt acggctggcc gggaccaacg tcgatctggg taagaccatc
     3121 ctcgccaggt cgggcctgcc gatcgtcgct gccgacaatc tggaggatgc cgccgagaaa
     3181 attgtcaagg ccgtgcggga ggctgcatag atgtcggttc ttgttaacgc cgcgacgaaa
     3241 gtcatttgcc agggctttac cggggcgcag ggcacctatc actccgaaca ggcgatcgcc
     3301 tacgggacga agatggttgg tggtgtgacg ccgggaaagg gcggcacgac gcacctcgac
     3361 ctgccagtgt tcaacacggt gtgggagtgc cgggaagcga ccggtgctga cgccagcgtg
     3421 atctacgtac caccgccgtt cgccgcggac gcgatcctgg aagcgatcga cgcggaattc
     3481 ccgctgatca tctgcatcac cgagggcatt ccggtgctgg acatggtgcg ggtaaagcgc
     3541 gccctgagcg gctcgaagtc ccgcctgatc ggaccgaact gcccgggcgt gataaccccg
     3601 gacgagtgca agatcggcat catgccaggc cacatccata ggcgtggcac cgtcgggatc
     3661 gtgtcgcgct ccggcacgct gacctatgag gctgtggcgc agaccaccgc ggctgggctg
     3721 ggacaaacaa gttgcgtcgg catcggtggc gatccggtaa agggcaccga cttcatcgag
     3781 gtgctggaga tgttcctggc cgacccggaa accaagcaaa tcgtcatgat cggcgagatc
     3841 ggcggcagca gcgagaccga tgccgcggag ttcctgtcca ggaacaaggt gaagaagccg
     3901 accgttggct tcattgccgg cgcggcggcg cctccgggcc gccgcatggg tcatgcgggc
     3961 gcggtgatca gcggtggcaa ggacaccgcg gcggccaaga tggatgcaat gcgcagtgcc
     4021 ggtatccacg tcgcggacag cccggccgcg ctaggcagca cgatggtcga ggtgctgaag
     4081 ggttagggcg cgagtggctg acggctgcgg cgagcctgct ttccttatga ctggttctgg
     4141 gataggatcc tgcttggata tcggctggaa cgagtgacgg gttcggcgat gtctttgcct
     4201 tggctcaacg tcgccagatg ctttctggct ttctcggcct ccattgctcg tctgcatctt
     4261 gcacggctcg cgagacttgc cggagatttt gcgcgacctc tgatgggtaa tttggcacga
     4321 ttcagccccg gtggtcgcga cccgctaccc atccgctttc gcacgaaccg gaaccgacat
     4381 ggacaagacc ctgcacgaca ggctgacggg atggcggcgt tacttgcacg cacatccgga
     4441 actcaccctg catgaacgag aaaccgctgc cttcgtccag gcgcggctgg ctgaacttgg
     4501 ggtcccgttc acttccggaa tcggcggcaa cggcgtcgtc gcgaccatca gccgcggtca
     4561 gtccaaccgc tccgtcgggc tgcgcgccga catggatgcg ctgccgatcc tagaaaccac
     4621 cggcctgccg cacgcttccg tcagcccggg cgtgatgcac gcgtgcggcc atgacggcca
     4681 taccacctcg ctgcttggtg ccgtggctct gctgtcggcc gatacgggct ggtccggcac
     4741 ggtccatttc gtcttccaac cagccgaaga aggcggcggc ggcgccaaga gtatgatcgc
     4801 cgacgggctg ttcgagcgtt tcccgatgga gcgcatcttc ggctaccata actggccggg
     4861 actggaggca ggcaccgttg ccgtccacga cggtccggtg atggcggccg gcaatcgggt
     4921 cttgttcacc gttaccggcc acgccggcca cgcggcgctg ccgcatctga cacgcgaccc
     4981 gatggtcgcc tccgcccatc ttctgctggc gttgcaatcg atcgtgtcgc ggaccgtcga
     5041 tccgttggac agtgccgtgg tcacgatcgg cacgatgcaa gccggatcgg cggccaacca
     5101 gatctcggac cgagcggtga tgcgcggcac gatccggaca ttgcggaacg aagtcgccgc
     5161 ccaggtgaag caggcaatcc accggatcgc agagggagtc gggcacagct tcggtgtctc
     5221 catcgacgtg gagatcccgc cgggcaacct ggtgactgcg aacacgccag ccgaacgcga
     5281 tttggcagcc gaagcggtgg cctcggccgg gctgaagctg cgccgggaca tggcgccggc
     5341 gatgaccggc gaagacttct cctggttctt gcagcagcgg ccaggagcct tcgtttggat
     5401 tggcaacggc gactcggccg gcggccgcga actgcacaat tcgaattatg atttcaacga
     5461 cgccattctg cccgccgcgg cgggatacct cgcctcggtc gcccggcgcg cgctgggata
     5521 ggccgcttgt caaaacacgg gtccatgcca acgtcaggga atctcataac cgcacaagga
     5581 cgagcgccat ggctgaaatt cccgcccagt ttctcgatct gctgaccgag aagaaggcat
     5641 tcgcgcacat cgccacgctg cagccggacg gatcaccgca agtcacacca gtgtggttcg
     5701 atttcaccaa tggtgttgtt cgcgtcaaca cggcaaaggg ccgggtaaag gcgcgcaatc
     5761 tatctgaagg ttccaaggtg gcgctgtcga tcatcgatcc ggacaacgcg tatcggtaca
     5821 ttcagatccg cgggacggta acgaaggaaa ccacggacgg tgcggtcgcg cacatcgata
     5881 gcctggctaa gaagtatctc ggcaaagacg tctatccgtg gcacagcgac aaagaaacgc
     5941 gggtgatcta cgagattacg ccctcagcca cgcacacgat gggtt
//
LOCUS       DASWQN010000498         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1102155, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000498 DASWQN010000000
VERSION     DASWQN010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102155"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="VGC82_08230"
     CDS             complement(<1..1390)
                     /locus_tag="VGC82_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEY0423294.1"
                     /translation="MSTVARARSGQIPVFGFLDRSRIIAGPGFNRWLVPPAALAIHLC
                     IGMAYGFSVFWLPLSRVIGGGKPASCPAGSGIMNALFTTSCDWLVADLNWVFTLFIFF
                     LGVAAALWGGWLERVGPRKAAVVSAICWSGGLLFGAVGIVTHQLWILWLGTGVLGGIG
                     LGLGYISPVSTLIRWFPDRRGMATGMAIMGFGGGAMIGAPLAQMLMEYFRSPGQGGVW
                     QTFVVMGLIYFVFMMGGAFGYRLPPPGWQPAGWTPPAPSAKAMITRGQVHLKDAHKTA
                     AFWCIWVVLCMNVSAGIGVLSMASPMLQEIFGGSLIGQPGVSFTALSDSQRVAVAAIA
                     AGFTGLLSLFNIGGRFFWASLSDHIGRKATYFVFFLLGIAMYAAAPWAAHAGSQTLFV
                     LFFCVILSMYGGGFATVPAYLADMFGTQFVGAIHGRLLTAWSTAGIIGPVLVSYMREA
                     AISAGVARSSVYD"
     gene            complement(1453..>2029)
                     /locus_tag="VGC82_08235"
     CDS             complement(1453..>2029)
                     /locus_tag="VGC82_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY0423295.1"
                     /translation="SALDDLTRRQRRLAGPSGCGLCGLDSLAAAIRPAPAVPVGRTFS
                     PGMIQDAMRSMPSAQILNVATHAVHAAAYWSPEQGLHVLREDVGRHNALDKLSGALAR
                     LAVIVAEGLVLLSSRVSVEMVQKAATLGAPVVVAISAPTSLAVEVAEGAGITLIGVAR
                     SDGFEVFTHPNRISSYPSAWRPAEKAENRVA"
BASE COUNT          441 a          681 c          613 g          294 t
ORIGIN      
        1 cgtcatagac cgaggatcgg gccacgcccg ccgagatcgc cgcttcccgc atgtagctga
       61 ccagtaccgg cccaatgatc cccgcggtgg accaggcggt cagcaggcgg ccgtggatcg
      121 cgccgacgaa ctgggtcccg aacatgtccg ccaggtaggc cgggactgtt gcgaacccgc
      181 cgccatacat cgagaggatg acacagaaga acagcacgaa cagcgtctgg cttccggcgt
      241 gcgcggccca gggtgccgcc gcatacatcg cgatacccag caggaagaac acgaaatagg
      301 tcgccttacg gccgatatgg tcggacaggg acgcccagaa gaaccggcca ccgatgttga
      361 acagcgatag caagccggta aatccggccg caatcgccgc cacggcaacg cgttgcgaat
      421 cgctcagtgc agtgaaggat acgccgggct ggccgatcaa cgagccgccg aaaatttcct
      481 gcagcatcgg cgaggccatg ctcagcacgc cgatgccggc cgacacgttc atgcagagca
      541 ccacccagat gcaccagaac gccgcggtct tgtgcgcgtc cttcaggtgc acctgacccc
      601 gggtgatcat cgccttggcg cttggcgcag gcggcgtcca gccggccggc tgccaacccg
      661 ggggaggtag ccgatacccg aacgccccgc ccatcataaa gacgaagtag atcaggccca
      721 tcacgacgaa ggtctgccag acgccgcctt gccctggcga gcggaagtat tccatcagca
      781 tctgcgccag cggggcgccg atcatcgcac ccccgccgaa gcccatgatc gccatgccgg
      841 tggccatgcc gcggcggtcc ggaaaccatc ttatcaacgt cgatacgggc gagatataac
      901 caagcccaag gccgatgccg cccagcacgc cggttcccag ccacaggatc caaagctggt
      961 gggtgacgat gccaacggcg ccaaacaaca ggccgccgga ccagcatatg gcggacacaa
     1021 cggcagcctt acggggccca accctttcga gccagccgcc ccagagcgcc gcggcaacgc
     1081 ctaggaagaa gatgaacagc gtgaagaccc agttcaggtc ggccaccagc cagtcgcagc
     1141 ttgtggtaaa caaggcgttc atgatgcctg atccggcggg gcaggaagcg ggcttgcccc
     1201 cgccgatgac acgcgacagc ggcagccaga agacgctgaa gccataggcc atgccgatgc
     1261 agagatggat cgcgagcgca gccggcggaa ccagccaccg attgaaccct ggaccagcaa
     1321 tgatccgact tcgatctaaa aatccaaaaa caggaatttg gccgctgcgc gcccgagcca
     1381 ccgtactcat gaagtttatc tcccaggcca ggaattttct tatatctaag tagattaaag
     1441 atgtcggcgg cgtcaggcta cgcggttttc ggctttctct gccggccgcc acgctgatgg
     1501 gtaactactg atcctgttcg gatgtgtaaa gacttcaaac ccgtcggacc gggccacgcc
     1561 aattaacgtg atgccggcgc cctcggccac ctcgaccgcc aatgaggtgg gggccgagat
     1621 ggcgacaacg accggggcgc ccagcgtcgc cgccttctgt accatttcga ccgagacccg
     1681 gctggacagc agaaccagcc cttcggccac gataacggct aaccgggcca gcgcgcccga
     1741 gagcttgtcc aacgcgttgt gacggccgac gtcctcacgc agtacatgca gtccctgttc
     1801 gggcgaccaa taagccgctg cgtgaacggc atgggtggca acgttcagga tctgggctga
     1861 cggcatggat cgcatggcgt cctggatcat cccgggactg aacgtccgcc ccaccggaac
     1921 cgcgggcgcc ggccggattg ccgcggccag actgtccaac ccgcacaggc cgcagccact
     1981 tggaccagcc aaccgccgct gccgcctggt caagtcgtcc agtgcgcta
//
LOCUS       DASWQN010000499         3602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2422033, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000499 DASWQN010000000
VERSION     DASWQN010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2422033"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VGC82_08240"
     CDS             <1..537
                     /locus_tag="VGC82_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423296.1"
                     /translation="FFLAAAGGNPQIAKAAIRELIEAYNAATPIELDLVGRIIGFSTV
                     AMDNLRLSMTPGLSDTKILRYRSNAATLSRSSDNARTMLEALQAGRAAKRDVPRPSVA
                     TAPRAPLQTRLPIKPAEQVSDMATDLEAMKRDARIMMAAFSKTGSVPPLNPPEMIRAA
                     ARDAVNSIQAASADPPRW"
     gene            complement(548..2407)
                     /locus_tag="VGC82_08245"
     CDS             complement(548..2407)
                     /locus_tag="VGC82_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY0423297.1"
                     /translation="MAHGISAGLTLAPRRVASNVDRALRLVAEGPAALLVLAEIVVLF
                     AGVTARYAFHAPLIWTDELASILFLWLAMLGSVIALQRSEHMRLTAIVGGMSPAGRAR
                     TEALASAAVAVFLLMILPSAWDYASDEWFIETPALGIHNTFRAMAIPVGAALMVVTAL
                     NRLLRLSLKDALFGVAVVAALGLVLHFAGPWMKSFGNWNLVLFFVLLLGAGVLLSIPI
                     GFAFGLATLAYLATLTRTPLTVVVSRMDEGMSSLILLAVPLFVFLGLLIEMTGMARAM
                     VTFLSSLLGHVRGGLSYVLLGAMYLVSGISGSKAADMAAVAPVLFPDMKRRGANEGEL
                     VSLLSASGAMSETIPPSLVLITIGSVTGVSIAALFTGGLMPALVLAAALAFIARWRGR
                     NENMAGVVRPPWSVIGKAFVISLPALVLPFVIRTAVIEGIATATEVSTIGIVYAVIVG
                     ILVYRQFPLHRLYPMLVETAALSGAILLIIGTATGMAWALTQSGFSRQLAAAMTQMPG
                     GAVGFMAISIVAFIVLGSVLEGIPAIVLFGPLLFPSAKLVGIHEVHYAMVVILAMGIG
                     LFAPPFGVGYYAACAIGRVSPDAAMRRIWPYLLAVLAGLIVVAAVPWFSIGFL"
     gene            complement(2423..3430)
                     /locus_tag="VGC82_08250"
     CDS             complement(2423..3430)
                     /locus_tag="VGC82_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY0423298.1"
                     /translation="MPMLDRRTLLAGAALPLFAIGSGRARAAEFNLKLANNAPMTHPQ
                     TIRQQEAIDRIKTATNGQVEIQLFPNNQLGSDTDMLSQLRSGAIDFFTLSGLVLATLV
                     PPASINGIGFAFKDYDTVWKAMDGKLGAHVRAEIDKRGLIAMDKMWDNGFRQITSSTH
                     PIKEPADLRGFKIRVPVSPLWTSMFQALGASPISINFSEVYSALQTKIAEGQENPLTL
                     IQIAKLYEVQKYVSMTSHMWDGFWMLGNKRGFSALPPDTQAIIARELNKSALDERADL
                     AKLNGSVADDLKSKGLEFVEVDKPAFREALKTAGFYAEWKKKYGDEAWNILESEVGSL
                     T"
BASE COUNT          740 a         1277 c         1040 g          545 t
ORIGIN      
        1 ttcttcctgg ccgccgccgg tggcaatccg caaatcgcca aggctgccat cagggaactg
       61 atcgaagcat acaacgccgc cactccgatc gaactggacc tggtcggccg catcatcggc
      121 ttctccaccg tcgcgatgga caatctccgg ttgtcgatga cgcccggcct gtccgacacc
      181 aagatcctgc gctaccgatc caacgccgcc accctcagcc ggtcgtccga caacgccagg
      241 acgatgctgg aagcgctcca ggccggccgc gcagccaagc gcgatgttcc gcgcccatcc
      301 gtcgcaaccg ccccaagagc cccgctgcag acgcggcttc cgataaagcc ggccgaacag
      361 gtatccgaca tggcgacgga tctcgaagcc atgaagcggg acgcccgcat catgatggcc
      421 gcgttctcca aaaccggctc tgtgccgccc ctgaacccgc ccgagatgat acgagccgcc
      481 gcaagggacg cagtcaattc catccaggcg gcatccgccg acccaccacg ctggtgatca
      541 gacctgccta caagaacccg atactgaacc aaggcaccgc cgccaccacg atcaagccag
      601 ccagcaccgc cagcaaataa ggccagatcc gtcgcatcgc cgcatccggc gacacccgcc
      661 cgatcgcgca ggccgcgtaa tatcccacgc cgaacggcgg cgcgaacaac ccgatcccca
      721 tcgccaggat caccaccatc gcgtagtgaa cctcgtggat ccccaccagt ttcgcggacg
      781 ggaacagcag cggcccgaac agcacgatcg ccggaatccc ctccagcacg ctgcccagca
      841 cgatgaacgc cacgatcgag atcgccatga accccacggc cccgcccggc atctgcgtca
      901 tggccgccgc caactggcgg gaaaatccag actgtgtcag cgcccacgcc atcccggtcg
      961 ccgtgccgat gatcagcagg atcgcccccg acagcgccgc ggtttccacc agcatcggat
     1021 acagccggtg cagcgggaac tgccggtaca ccaggatgcc gacgatcacc gcataaacga
     1081 tgccgatcgt ggaaacctcg gtcgccgtcg caatgccctc gatcaccgcg gtgcggatca
     1141 cgaacggcag caccaacgca ggcaaggaaa taacgaacgc cttgccgatc acgctccagg
     1201 gcggccgcac gacgcccgcc atgttctcgt ttcgaccgcg ccagcgcgcg atgaacgcca
     1261 gcgccgccgc cagcaccagc gccggcatca acccgccggt gaacagggcc gcgatggaaa
     1321 ccccggtcac cgacccgatg gtgatcagca ccagcgatgg cggaatggtc tcgctcatgg
     1381 cgccggatgc cgacagcagc gaaaccaact ccccctcatt ggcgccccgg cgcttcatat
     1441 ccgggaacag caccggcgcc accgccgcca tgtcggccgc cttggagccg gaaattccgg
     1501 aaaccaggta catcgccccc agcagcacat acgacagtcc accgcgcaca tgccccagca
     1561 gcgaggacag gaaggtcacc atcgcccgcg ccatgccggt catctcgatc aatagcccga
     1621 gaaacacgaa caacggcacc gccagcagga tcagcgaact catgccctcg tccatgcgcg
     1681 aaaccacaac ggtcaagggc gtccgcgtca gcgtggccag atacgccagc gtcgccagac
     1741 cgaaggcaaa cccgatggga atcgacagca ggactcccgc acccagcagc agcacgaaga
     1801 acagcacgag gttccagttg ccgaagctct tcatccaggg cccggcgaag tgcagcacca
     1861 accccagcgc ggcaaccacc gcgaccccga acagcgcgtc cttcaaagac agccgcaaca
     1921 gccggttcag cgcggtgacg accatcaaag ccgcccccac tggaatcgcc atcgctcgga
     1981 acgtgttgtg gatacccagg gcaggggtct cgatgaacca ctcgtccgag gcatagtccc
     2041 atgccgacgg caggatcatc agcaggaaaa ccgccactgc ggcggaggcc aatgcctcgg
     2101 tcctggcccg tcccgcaggc gacatcccgc ccacgatggc ggtcagccgc atatgttcgg
     2161 agcgttgcag ggcgatcacg ctgcccagca tcgccagcca caggaacagg atcgaagcca
     2221 gctcatccgt ccaaatcagc ggcgcatgga aggcgtagcg cgcggtgacg ccggcgaaca
     2281 acacgacgat ttcggccagc acaagcaagg cggccggccc ctcggcgacc agccgaagcg
     2341 ctcgatcaac gttggaggcc acccgcctgg gagccagggt aaggccggcg gagatgccat
     2401 gcgccatcta agcccgcccc cctcaggtca gagagccgac ttcggattcc aggatattcc
     2461 aggcctcgtc gccatatttc ttcttccatt cggcgtagaa accggccgtc ttcagcgcct
     2521 cgcggaacgc cggcttgtcc acttcgacaa actccagccc cttggacttc aggtcatcgg
     2581 cgaccgaccc attcagcttg gcaagatcgg cccgctcatc cagcgccgac ttgttgagtt
     2641 cccgcgcgat gattgcctgc gtatccggcg gcagcgccga aaatccgcgc ttgttgccaa
     2701 gcatccagaa cccgtcccac atgtggctgg tcatggagac gtatttctgc acctcgtaca
     2761 gcttggcgat ctggatcagg gtcagcgggt tttcctgccc ttcggcgatc ttggtctgca
     2821 gggcggaata gacctcggag aaattgatgc tgatcggcga cgcccccaaa gcctggaaca
     2881 tgctggtcca cagaggggac accggaacgc ggatcttgaa gcccctcaga tcggcgggct
     2941 ccttgatcgg atgcgtagag ctggtgatct ggcggaagcc gttgtcccac atcttgtcca
     3001 tggcgatcag gccccgcttg tcgatctccg cccgcacatg ggcgccgagc ttgccgtcca
     3061 tcgccttcca caccgtgtcg tagtccttga aggcaaaccc gattccgttg atcgaggccg
     3121 gcggcaccaa tgtcgccagg accaggcccg acagcgtgaa gaagtcgatg gcccccgacc
     3181 gcaactgcga cagcatgtcg gtgtcggagc ccagctggtt gttcgggaac aactggatct
     3241 cgacctggcc gttggtcgcg gtcttgatcc ggtcgatcgc ttcctgctgg cggatcgtct
     3301 gggggtgcgt catcggcgcg ttgttcgcca gcttcaggtt gaattccgct gcacgggcgc
     3361 ggccggagcc gatggcgaac aagggcagcg cggccccggc gagcagggtg cggcggtcga
     3421 gcatgggcat gaaggatcct tgggcgtttc tgttgtcgcg gaaaatacaa gctggccccc
     3481 cgatagcaag aggctcggac cgctgtggga tgaccagccc tctgtgtcat gactgtgcct
     3541 ccgcgtcatg gtccttcttt cgtgtcacga cccgacttgt cttcgtgtca tggcccggtt
     3601 tg
//
LOCUS       DASWQN010000500         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_179333, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000500 DASWQN010000000
VERSION     DASWQN010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179333"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1062
                     /locus_tag="VGC82_08255"
     CDS             310..1062
                     /locus_tag="VGC82_08255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0423299.1"
                     /translation="MDLKGAVALVTGGNGGLGQRICHALAQQGAHIAVMYAQSRDQAE
                     DVVRELTSRYQINAASFACDITDASAVETLIEGMTNRFARIDILINDAAYNKSIPFTD
                     LDNLTLDVWEKIMAVNLTGPMRLMKAVAPIMKAQGKGRIVNIASVAGLTPTGSSIAYA
                     VSKAGLVHLTRCMAVAMAPETLVNCVAPGLLEGTRATSNLRSEQIERSASGSLLKKAA
                     DKDDCADMVVTMCRTETMTGQTVVIDSGRVFH"
     gene            1320..>1571
                     /locus_tag="VGC82_08260"
     CDS             1320..>1571
                     /locus_tag="VGC82_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY0423300.1"
                     /translation="MMIIQGEGDNAMRIGFVGTGTMGTPIAGCLMRAGHPLAVYDRHQ
                     DATASLCSQGATRADTAFALARDSDVVFTSLPGPIEFESA"
BASE COUNT          310 a          500 c          466 g          295 t
ORIGIN      
        1 ctggacgttc caccagcatc ctgtgatgca gcctgccggg ggccagtgaa gtacgccgga
       61 tcagcccttt agctaaatcg aacggcacgt tcgagatgat cgatgctcaa tatggctacg
      121 gtcgtgatga agccggcatc aaccgatgca actgcttgca ccctacaaca ctccgccaac
      181 ggtcgaagct gccgccaacc gagtcccgaa tgctcgccgt tcaagtcaac ggcttgcctg
      241 aggcttggat cgggtaacaa actatctgcc aacgcgggac agtgccgcgg aacaggcagg
      301 agaaaccaca tggacctcaa aggcgccgtg gcgctggtga ccggcgggaa tggcgggctc
      361 gggcagcgca tctgtcacgc gctggcgcaa cagggtgccc atatcgccgt catgtatgcc
      421 caaagccgcg atcaggcgga ggacgtcgtc cgcgagttga cctcgcgcta tcagatcaac
      481 gccgcttcat tcgcctgcga catcacggac gcctccgccg tggagacgct gatcgaaggc
      541 atgacaaatc gcttcgcccg catcgacatt ctgatcaacg acgccgccta caacaagtcg
      601 atccctttta ccgacctgga caatctcacg ctcgacgttt gggaaaagat catggcggtc
      661 aacctgacag gaccaatgcg cctcatgaag gcggtggcgc ccatcatgaa ggcccaagga
      721 aagggccgca tcgtcaacat cgcgtcggtt gcaggtctta caccgacggg ctcttccatc
      781 gcctatgcgg tgtcgaaggc gggcctcgtc catcttacgc gctgcatggc agtggcaatg
      841 gcgcccgaaa ctctcgttaa ttgcgtcgca ccgggcctgc tggagggcac acgagcgact
      901 tccaatctcc ggtccgagca gatcgagcgg tctgcgtccg gctcgttgct gaagaaggcc
      961 gccgacaagg acgattgtgc cgacatggtg gtcacgatgt gccgcaccga gaccatgacg
     1021 ggccagacgg tggtcatcga ctccgggcgc gtgtttcact gaaatgcggc gatgtccgca
     1081 ccaccatccc catggaggat attgcctttc ggatgtctat gcgaaccgta gggaatcttc
     1141 atagacgccg ctcatggcta catccttgct atgggcggtg tcagcaatcc agatagtttg
     1201 tccagctacc gcgccccggc cttgccctaa ggccagacag ctttcggcct ccaagcgagc
     1261 ccccccgccg gtcacgccgc tgtcggtcac gccgcttgtc atccatgatc cgccggccga
     1321 tgatgattat ccagggggag ggcgacaacg ccatgcggat cggcttcgtc ggaaccggga
     1381 ccatggggac gccgattgcg ggctgcctga tgcgcgccgg tcatccgctt gcggtttatg
     1441 atcggcacca ggacgccacc gcttcgctgt gctcgcaagg tgccacgcgc gccgataccg
     1501 cattcgcctt ggcgcgcgac agcgacgtgg tgttcacctc gcttcccggt cctattgaat
     1561 ttgagtcggc g
//
LOCUS       DASWQN010000501         3799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1367193, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000501 DASWQN010000000
VERSION     DASWQN010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367193"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VGC82_08265"
     CDS             complement(<1..294)
                     /locus_tag="VGC82_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEY0423301.1"
                     /translation="MHIAKVPNRNARPSFLLRESYREDGKVKNRTLANLSKLPVERID
                     TLRAALRGDPLVPIGDNGFEIRRSLPHGHVLAALTTAGRIGLDDLLPRRAPQRR"
     gene            complement(537..1668)
                     /gene="prfB"
                     /locus_tag="VGC82_08270"
     CDS             complement(join(537..1598,1600..1668))
                     /gene="prfB"
                     /locus_tag="VGC82_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438812.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY0423302.1"
                     /translation="MSAESDALNEQIKQSIVLLRRHLDWDVAESRLADLNNRAEDPAL
                     WNDAAAAQKLMRERNQIASAMEGVQKLEAEVADALELIGMAEAEGDSAMVSDAMASLR
                     AAADEAKRREIESLLSGEADRNDCFIELNAGAGGTEAQDWAQMVMRMYMRWAEQHDYK
                     VQLMEESEGEQAGIKSATLQVSGPNAYGWLKTEGGVHRLVRISPFDANARRQTSFASV
                     WVYPVVDDSIDIEINEADLKVDTYRASGAGGQHVNKTESAIRITHVPSGIIVACQTDR
                     SQHRNRATAMAMLKARLYEAELQRREAISAAQESAKTDIGWGHQIRNYVLAPYQLVKD
                     LRTGIETGNPAAVLDGDLDEFMAAALAARSGATRSEASAQAR"
     gene            complement(1700..>3799)
                     /locus_tag="VGC82_08275"
     CDS             complement(1700..>3799)
                     /locus_tag="VGC82_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY0423303.1"
                     /translation="NMLLTNQMSLARKAKEAILALRIEQNVSKDRILELYLNEIYLGL
                     GSYGVAAASQAYFNKPLDKLTTAEAAFLAALPKAPNNLNPFKYPDAARSRRDYVLDRL
                     AEDHAITPVQATEAKATAVVPSEFRRPPPIPGADWFTEEVRRQLVARFGQESATQGGL
                     MVRTSLDPALQLAAEKSLRDGLMGYDRRMGGWRGAVAHLSLPAADFETKWPAALNEQP
                     RPPGMLPAWRLAVVAGMTDTEAKVEWLNPENERRAAALALPDVTWARTVREGKPGPVP
                     RRMSDIVQVGDVLMIEPPSVQPSPPSVPPPPVKGKAPPPTTPLPANRATLRQIPQVQG
                     ALVSLDPRTGRVLAMVGGWSFEQSQFNRVTQASRQPGSSFKPMVYLTALQKGVSPSQR
                     YLDAPIVVDTPEGRWRPGNYEGTFGGPTSLRVALEESMNLVTLRVAQQVGMQAIADNA
                     IAFHMVDSMPRVLPAALGAVETTVMREAGAYASIAAGGREVTPTVIDSVQDPDGHVVM
                     RASGLDCSACSDPSKPPAVTDQRAQIADPESVFQLITMMQGVVQRGTGVPAGKGLNRP
                     IAGKTGTTQDFADAWFAGFTPDLATVVWVGYDSPASLGEKQTGGAVAAPIWHDFMAAA
                     LAGHPVLSFAMPPGMTMAAWDSGSGMVTDAFKADQVPGASAPIGMGVASAPSADGSPP
                     QPTIVHGGVDNSMGGLY"
BASE COUNT          613 a         1298 c         1191 g          697 t
ORIGIN      
        1 gcggcgctgg ggcgcgcggc ggggcagcag gtcgtcgagg ccaatgcgtc cggcggtggt
       61 cagcgcggcg aggacgtggc catgcggcag cgagcggcgg atttcgaagc cgttgtcgcc
      121 gatcgggacc agggggtcgc cacgcagcgc cgccctcagg gtgtcgatgc gctcgaccgg
      181 cagcttggac aggttggcga gggtccggtt cttgaccttg ccgtcctcgc ggtaggattc
      241 ccgcagcagg aaggaggggc gggcgttgcg gttagggacc ttggcgatgt gcatgaggca
      301 gggaatcgca ccgagtccgc gtctgtcaag tacaaatttg ctatatgctt gactacatat
      361 tcgtagtctt tccttgctta tcaaacctct gggccgaaaa cagggcaaag ttcggtctag
      421 ccctcgccta ggctgcctta cgtccgtgga aatgcgacgc attgacatcg cgatggcacg
      481 gtttcttgga attgccggag tgaccaacga cctcccggcc gagtgactac gatcacctac
      541 cgagcctggg cactcgcctc cgaccgcgtc gccccggatc gtgcggccag cgccgctgcc
      601 atgaactcgt ccaggtcgcc atcgagcacg gcagccggat tgccggtctc gatcccggtg
      661 cgcaggtcct tgaccaactg atacggcgcc agcacgtagt tgcggatctg gtgaccccat
      721 ccgatatcgg tcttggcgct ttcctgcgcc gccgagatcg cctcgcgccg ctgcaactcc
      781 gcctcgtaca gccgagcctt cagcatcgcc atcgcggtcg ctcggttgcg gtgctggctg
      841 cggtcggtct ggcaggcgac gatgatcccg cttggaacat gcgtgatgcg gatcgcgctt
      901 tccgtcttgt tgacgtgctg cccgccggcc cccgaggctc ggtaggtatc aaccttaaga
      961 tccgcctcat tgatctcgat gtcgatcgag tcatccacca ccggatacac ccagaccgat
     1021 gcgaaactgg tctgccgccg ggcgttggcg tcgaacggac tgatgcgaac gaggcgatgc
     1081 acgccgcctt cggtcttcag ccagccatag gcattcgggc cgctgacctg caacgtcgcc
     1141 gactttatgc ccgcctgctc gccctcgctt tcctccatca actgcacttt gtaatcgtgc
     1201 tgctccgccc agcgcatata catgcgcatc accatctgcg cccagtcttg cgcctcggtg
     1261 ccgccggcac ccgcgttcaa ctcgatgaag cagtcgttgc ggtcggcttc accggacaac
     1321 aggctttcga tctcccgccg cttcgcttca tcggccgccg cgcgcagcga cgccatggca
     1381 tcggagacca tggccgagtc accttcggcc tcggccatcc cgatcaattc cagcgcgtcg
     1441 gcgacttccg cttcaagctt ctgcacgccc tccatcgccg aggcgatctg attccgctcg
     1501 cgcatcagct tctgcgcggc agcggcatcg ttccacaggg cggggtcctc ggcgcggttg
     1561 ttcaggtcgg cgaggcggga ctcggcgaca tcccagtcaa agatgcctcc tcagcagcac
     1621 gatggactgc ttgatctgct cgttcagggc gtcggattcg gcggacatgt caaaagatct
     1681 gcttgttgca aggggcgggt caatacagcc cgcccatgct gttatcaacc ccgccatgca
     1741 cgatcgtcgg ctgcggcggg ctgccgtcgg ccgatggagc gctggcgacg cccatgccga
     1801 tcggcgccga ggccccgggg acctggtcgg ccttgaaggc atcggtcacc atgcccgaac
     1861 cggaatccca ggccgccatg gtcatcccag gcggcatggc aaaggacagc accgggtgcc
     1921 ccgccagcgc ggctgccatg aagtcgtgcc agattggggc cgcgaccgcg ccgccagtct
     1981 gtttttcccc cagcgatgcc gggctgtcgt atccgaccca caccacggtc gccagatcgg
     2041 gggtgaagcc ggcaaaccag gcatcggcga aatcctgggt cgtgccggtc ttgccggcga
     2101 tcggccggtt gagccccttg cctgccggca cgccagtgcc gcgctgcaca acgccctgca
     2161 tcatggtaat caactggaac acactctcgg gatcggcgat ctgcgcccgt tggtcggtca
     2221 ccgcaggcgg cttcgatggg tcggagcaag ccgaacagtc cagccccgac gcccgcatca
     2281 ccacatgccc atctgggtcc tgcacgctgt cgattaccgt gggcgtgact tcccgcccgc
     2341 ccgctgcgat cgaggcatag gccccggctt cgcgcatgac ggtcgtttcc accgcgccca
     2401 gggccgcggg cagaacccgg ggcatcgaat ccaccatgtg gaaagcgatg gcgttatccg
     2461 cgattgcctg catcccaacc tgctgcgcaa cgcgcagggt caccaggttc atcgattcct
     2521 ccaaagcgac ccgcagcgat gtcggtccgc cgaaagtgcc ttcgtagttg cccggccgcc
     2581 agcggccctc cggcgtatcg accacgatcg gcgcatccag gtacctctgg ctcggcgaca
     2641 cgcctttttg cagggcggtc aaatagacca tcggcttgaa gcttgatcct ggctggcggc
     2701 tggcttgcgt gacgcggttg aactggcttt gctcgaaact ccacccgccg accatcgcca
     2761 gcacgcgtcc ggttcgcgga tcgagcgaca ccagtgcgcc ctgaacctgg ggtatctgcc
     2821 gcaacgtcgc ccggttggcc ggaagcggcg tcgttggcgg cggcgccttg cccttcaccg
     2881 gaggcggtgg caccgaaggc ggtgacggct gcaccgaagg cggctcgatc atcaacacat
     2941 cgccgacctg tacgatatcg ctcatgcgcc tggggaccgg tcccggcttg ccctcgcgaa
     3001 ccgtccgagc ccaggtcacg tcgggcagcg cgagggcggc ggcgcggcgc tcgttttccg
     3061 ggttcagcca ttcgaccttc gcttcggtgt cggtcatccc ggccacgacc gccaaccgcc
     3121 aggccggcaa catgccgggc ggccgcggct gctcattcag cgccgccggc catttcgtct
     3181 caaaatccgc cgcgggcagg ctcagatgcg cgaccgcacc gcgccagccg cccattcgcc
     3241 ggtcgtagcc catcaggcca tcgcgcagcg acttctcggc cgcaagctgc aacgccggat
     3301 ccaggctggt gcgtaccatc agtccgccct gagtcgcgga ctcctggccg aaccgcgcaa
     3361 caagctggcg gcgcacttcc tcggtgaacc aatcggcgcc ggggatcggt ggcgggcgtc
     3421 gaaattccga cggcacaacg gcggtcgcct tcgcttcggt ggcctggaca ggcgtgatcg
     3481 cgtggtcctc cgccagccga tccagcacgt agtcgcgacg cgatctggcg gcatcgggat
     3541 acttgaacgg gttcaggttg ttcggcgcct tgggcaaggc cgcaagaaac gcggcttcgg
     3601 cggtggtcag cttgtcgagc ggcttgttga aatatgcctg tgacgccgcc gccacgccat
     3661 aagaccccaa cccaagataa atctcgttca gatacagctc cagaatgcga tccttgctga
     3721 cgttctgttc gatccgaagc gccaggatgg cctccttggc cttccgcgcg agcgacatct
     3781 ggttggtcag cagcatgtt
//
LOCUS       DASWQN010000502         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1367266, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000502 DASWQN010000000
VERSION     DASWQN010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367266"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="VGC82_08280"
     CDS             <1..1474
                     /locus_tag="VGC82_08280"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEY0423304.1"
                     /translation="ITFRIATSTCPVGCPGALLGREEPNDATGQPRRRPMTDEIRLLS
                     TSAILGYGFPEASLKAGMDREPHAIGVDGGSVDPGPHYLGCGKPFCSPIAIRRDLRLM
                     LNAAVRANIPMVIGTCGGAGGAPHLDLVATMAREIAREDGLHFKMALIQAEQDKDSVK
                     RRVAAGRIKPLAHQPALTNETIDRSARIVGMMGPEPFIEALDAGAQIVLAGRASDPAS
                     WAAAATRAQLPPAPAWYAGKMLECGATPSIPKGHDSLLVTVRDDHVECEPMNPARRCT
                     PLSIANHSLHENSSPIYHIEPGGMLDTSECRFDAVSDRAVRISGMRWNPAETYTVKLE
                     GVELAGYRSICVCGTRDPLLIARLDDFIDSVRAEVATKAAAFGASPDSYQLGIRSYGR
                     DGVMGAREPLAGATPHELGFVLEVVSQQSQEMASAVLGMARTNMLHTDFPGRLCREGN
                     MAFPFSPSDIEVGPVYRFSIYHVAELDDPTEPFPIQYETL"
     gene            1476..1787
                     /locus_tag="VGC82_08285"
     CDS             1476..1787
                     /locus_tag="VGC82_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HEY0423305.1"
                     /translation="MALIKNVAKVCKSKNAGPFELTIDVVFDTAETFNRVKATGVLCA
                     ALFARLYNVPETHVLFTPYDAAFAFKATLPRLVPSGDFGDTDVYGCQQHAPLLDVDLP
                     I"
BASE COUNT          357 a          663 c          614 g          341 t
ORIGIN      
        1 aatcacgttc cgcatcgcca cgtccacttg ccccgtcggc tgcccgggtg cattgctcgg
       61 ccgagaggaa ccgaacgatg cgacaggtca accacggcgg cgccccatga ccgacgaaat
      121 ccgcctgctg tccaccagcg cgatccttgg ctacggcttc ccggaggcct cgctgaaagc
      181 cggcatggac cgagaacccc acgccatcgg cgtcgatggc ggcagtgtcg atccgggacc
      241 gcactacctc ggctgcggca agccgttctg ctcgccgatc gcgatccgcc gcgaccttcg
      301 cctgatgctg aacgcggccg tccgcgccaa cattccgatg gtgatcggca cctgtggcgg
      361 tgccggcggt gccccacacc tcgacctggt cgccaccatg gcgcgggaga ttgctcggga
      421 agacggcctg cacttcaaga tggcgctgat ccaggccgaa caggacaagg acagcgtcaa
      481 gcgccgtgtc gccgccggac gcataaagcc gctggcgcat cagccggcgc tgaccaacga
      541 aacgatcgac cgttcggcgc gcatcgtcgg catgatgggg ccggaaccat tcatcgaagc
      601 actggatgcg ggcgctcaga tcgtactggc cggccgagcg agcgacccgg cgtcgtgggc
      661 agccgccgcc actcgggcac agttgccgcc cgcgcccgcc tggtatgccg gcaagatgct
      721 tgaatgcggc gcgacgccct ccatcccgaa aggccacgac agcctgctgg tcacggtccg
      781 cgatgaccac gtcgaatgcg agccgatgaa tcctgcccgc cgttgcacgc cgctgtcgat
      841 cgccaaccat tcgctgcacg agaacagcag cccgatctat cacatcgaac ccggcggcat
      901 gctggacacg tccgaatgcc gcttcgatgc ggtcagcgac cgtgcggtgc ggatttccgg
      961 catgcgatgg aacccggccg agacatacac ggtgaagctg gaaggcgtgg aactggcggg
     1021 ataccggtcg atctgcgtct gcgggactcg cgacccgttg ctgatcgcgc ggttggacga
     1081 cttcatcgac agcgtccgtg ccgaggtcgc gaccaaagcc gcggcgttcg gtgcgtcacc
     1141 cgacagctac cagctgggca ttcgcagcta cggccgggat ggcgtcatgg gggcccgcga
     1201 accgctggcc ggcgccacgc cgcatgaact gggcttcgtg ctggaagtcg tctcgcagca
     1261 aagccaggag atggccagcg cggtcctcgg gatggcccgc accaacatgc tgcacaccga
     1321 tttccctggc cggctttgcc gcgagggcaa catggctttc ccgttctctc cgtccgatat
     1381 cgaggtcggt ccggtctatc ggttcagcat ctaccatgtg gcggagctgg acgatcccac
     1441 cgaacccttc cctatccagt acgagacttt gtgacatggc gctgatcaag aatgttgcca
     1501 aggtctgcaa gagcaagaac gccggcccgt tcgaactgac catcgacgtg gtgttcgaca
     1561 cggcggaaac cttcaaccgc gtgaaggcca cgggcgtgtt gtgcgcggcc ttgttcgccc
     1621 gcctgtacaa cgtgcccgaa acgcatgtgc tgttcacccc ttacgacgcg gcctttgcct
     1681 tcaaggcgac gttgccccgt ctggttccgt ccggggattt cggcgatacc gacgtttacg
     1741 gctgccagca gcacgccccg ctgctggatg ttgaccttcc gatctgaaag cgagacggca
     1801 cgatgaaccg catgatgagc ctggttgcgt ttttgcaggc acagaactgc tccaatctgc
     1861 cgggttcgtg gcggcaccct tcgaccgtgc tggatttcac cacgccggaa tattaccagc
     1921 gcatcgcccg gacgctggag gacggaaaaa tccaactggc gttcttcgac gatcg
//
LOCUS       DASWQN010000503        11258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2290669, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000503 DASWQN010000000
VERSION     DASWQN010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 11258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11258
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2290669"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..672)
                     /gene="purN"
                     /locus_tag="VGC82_08290"
     CDS             complement(25..672)
                     /gene="purN"
                     /locus_tag="VGC82_08290"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY0423306.1"
                     /translation="MKRRAAILISGRGSNMLALIAAARDPAFPAEIACVLSNRPDAAG
                     LTAARAAGVPAACIDHRPFSGDRAAHEAAIDAALRAANVEVVCLAGYMRLLTPFLVNE
                     WEGRMLNIHPSLLPSFPGLNTHARALAAGVKLHGCTVHLVTQEMDAGPILAQAAVPVL
                     PGDTEDELANRVLRQEHAIYPAALAAIAAGMLTTSPHPGTYLTNPLPWQASLTPR"
     gene            complement(669..1760)
                     /gene="purM"
                     /locus_tag="VGC82_08295"
     CDS             complement(669..1760)
                     /gene="purM"
                     /locus_tag="VGC82_08295"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY0423307.1"
                     /translation="MTTADNQGARPVLNDTGRLDYRAAGVDIEAGDALVEAIKPLAKA
                     TTRAGVMGGVGGFGALFDLKAAGFSDPVLVSTTDGVGTKLKIAVETALHGTVGIDLVA
                     MCVNDLVVQGAEPLFFLDYFATGKLSVAHASAVIAGIAEGCRLAGCALVGGETAEMPG
                     MYAEGDYDLAGFAVGAAERANLLPRPLTAGDHLLGLASSGVHSNGYSLVRRIVRASGL
                     GWNDAAPFAPGQTLGEALMTPTRIYVRSLLALHRAGLLKAAAHITGGGLPGNLLRVLP
                     ENTMAALQPAWPVPPVFHWLARTGGVGGHEMLRVFNCGVGMVVAVSDPEAAGLLLEAE
                     GETVSRIGAIEAADGPASVRIDPPAGWLA"
     gene            1858..2421
                     /locus_tag="VGC82_08300"
     CDS             1858..2421
                     /locus_tag="VGC82_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0423308.1"
                     /translation="MPDGPPARGTTASLLNLPNIVTFGRLCAVPLAFWLVLVHRVDQA
                     FLLFVAAGLSDALDGWLARRYGGNAIGAMMDPVADKALLVTMYITLATISALPAWLAI
                     LVVFRDLLIVGGIVVLAVLGHEVAIRPIYISKLNTLMQILLIAVILLQVGFGLGIPGL
                     DFVLTWCVATTTVASGAAYVWNTARGK"
     gene            2393..3586
                     /locus_tag="VGC82_08305"
     CDS             2393..3586
                     /locus_tag="VGC82_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0423309.1"
                     /translation="MSGTQPEENDVSAAGQDLLSPPKEPAVAAKEAAAAPPSAFHHAT
                     RTQRFALIIGLAVVSWLALALFASILAPFVAAGVLAYALDPPTTQLTRVGLPRGAAAL
                     LMIVGVIAAVLLFALLLYPLVIVQIGLLVNRIPQYATLLQGWAREVLTSLQDNFGPDV
                     VNDKLRDVVSGQAGSMLSILLSTVTGLIGGGFAIFNLLSLAVVTPVVGFYLLRDWPSV
                     VRMIDSWLPYRYREVIRAQAREVDRILSAWVRGQALCCLLLALYYAAGLTVAGLDLGL
                     IVGLMAGLLSFIPYVGSITGGVTAIGLALAQFPQWRGVAVIGVVLIIGQILEGYVIYP
                     RFLGDRVELPAVWVIFALFAGGAAFGFLGVMLAVPVAATIGVLARFWLRRYLASPLYL
                     DPPSS"
     gene            3586..4281
                     /locus_tag="VGC82_08310"
     CDS             3586..4281
                     /locus_tag="VGC82_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator DnaA"
                     /protein_id="HEY0423310.1"
                     /translation="MVRQPLQLPLPFQHEPRYDVRDFIPARSNQDALAWLDTDWPEQR
                     LALFGPPGCGKSHLLHIWAERSGAVLLAGQTLADLDGLPMRGSLALDDADTVADETLL
                     FHLLNTARDRGLRLLLSAGVAPSRWPVRLQDLSSRLRAITAIEIGQPGDDLLAMLLVR
                     LLADRQLGVSQAVQDWLLVHLPRSPSVLRLAVARLDRASLAFGSAITRPLAARMLSDL
                     VGAVPDDVCVTER"
     gene            4410..6713
                     /locus_tag="VGC82_08315"
     CDS             4410..6713
                     /locus_tag="VGC82_08315"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEY0423311.1"
                     /translation="MAHKADAADLMERVETSGKKPLVARTGAAKTAFGKAASEKPGTP
                     KPERPPEPIVDLAAPERFINRELSWLDFNHRVLEEAENPRHPLLERLRFLSISASNLD
                     EFYSVRVAALIGQAKAGATFTSADGRTAAQQLTAIKLRAESLLVDQQRILRELLGLLR
                     DAGVVLCTTDNLSENEKTWLEAWFMERVFPVVTPLAVDPAHPFPFISNFGLVLALHLV
                     REDDGQGMRGLLPLPSQIDRFVRLPAAESEESVRFILLDDLVVLFLGRVFPGFRMTGH
                     GLFRVIRDTDVEFEEEAEDLVRSYETALKRRRRGVAIHLEIAAGMPPELRELVIDELD
                     AAADEIHEDQHLLGLADMKQMIVDDRHDLLFAPYTPRFPERIRDFGGDCFAAIRAKDI
                     IVHHPYESFDVVVQFLRQAAQDPAVIAIKQTLYRTSRDSTIVNALIEAAEAGKSVTAM
                     VELRARFDEEANIRLSRRMEAAGVQVVYGFTGLKTHAKLSLVVRREAGAIRSYAHFGT
                     GNYHPITARIYTDLSFFTTDPALTRDSARLFNFMTGYARPEVMEELGFSPLTTRTKLL
                     ALIHREAAFARDGKPATIWLKMNSLTDVELIDALYEASGTGVRIMAVIRGICCLRPGV
                     ARLSENIRVKSIVGRFLEHGRIYAFGNGHPLPSRHARIFISSADWMERNMDWRVETLV
                     PIHNPTVHAQVLDQIMVINLKDSLQSWELNAEGAWNRVAPGRKPLSAHEYFMTNPSLS
                     GRGSALHGPAVASPVVAPRRQDRLTQD"
     gene            6716..8215
                     /locus_tag="VGC82_08320"
     CDS             6716..8215
                     /locus_tag="VGC82_08320"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEY0423312.1"
                     /translation="MLFDRPVRLPRCGVVDLGSNSVRLVVYEGHTRNPTPIFNEKAVL
                     RLGRGLQSTGRLNEEGMAQALTVMHRYHAVARAMDAEPFEVLATAAVRDADNGPAFVQ
                     ALRDRLPGVPITILSGVQEAAYSADGMLCGIPTADGLLADIGGGSLEVVRLVNGTRGP
                     SQTLKLGVIRLAERSGGDPTRARAIAQADLEAVPWLTEASGRDLYLVGGAWRALARIH
                     MAQIDYPLSMVHHYVIGREQARDLTELIAGASKKVLERLPGVPRRRIDDLPFAAVALR
                     RLLRVTNVGRVVFSASGLREGWFMRRMPRQIQEQDPMLSAAQEMAARYGRDGDLPPSL
                     LDWTRPLFAAENLASTQLREAMCWMSDIGSHDHPEFRAEQAFLRVFRQPGVGLDHPTR
                     AFMALAVALRYEAEPDAPFLRPARLLLDAATAAQAEVLGIALRLAYTLSAGTKDLLGG
                     TELRIEGPRLVLRLRTGSGVFAGESVIRRLERLAQALGLQAAAEEHLAT"
     gene            8212..8679
                     /locus_tag="VGC82_08325"
     CDS             8212..8679
                     /locus_tag="VGC82_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0423313.1"
                     /translation="MIRRAHATQAGAVRKVVLAAYRRYVAVIGTAPAPMLDDYATRIA
                     TGQVWIWQDADEVAGVLVLENGPDCFLLNNVAVRPDRQGQGIGRSLLDFSEAEARRCG
                     WDAITLYTNALMAENIAIYAARGYIERARRTENGFDRVYLEKRLDGKALDAGR"
     gene            8847..10562
                     /locus_tag="VGC82_08330"
     CDS             8847..10562
                     /locus_tag="VGC82_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY0423314.1"
                     /translation="MSATLPKIRRRNLPFDPLVILWVVLAAALTLLVVNPLFRLVQTS
                     LQDTDTGAFTWMNYVTAYSRPRYITAMLNSLRLGAGVTGLCVLFAVPIAWAVSRTDMP
                     GKGIVRFLVLGAFVTPPYLGAIGWILLAGPNSGWLNRIWMSLTGAQEGLFNIYSFGGL
                     TFNIAIYSFPYLFIFTTAALDLVSSEMEDAANILGAGILRTTLRVTLPMALPAILGGA
                     IVTFLEAIALFGSPALIAIPARFNVVTTQLLQFFSQPVRAEVAAAYAVPLLLITVLLF
                     GLQRLLVRRRGFVALTGKGGERRVMEIGPWRWVMLGYALFILSLAVLLPYIVLGLAAL
                     SKAWGRGMSLDNFTLHNFTYLLFQHETAARSVLNSFTYAAAAACIAIVLGLSIAYIAS
                     RRLLPFAGVLSFLCMAPFVVPGIVLAIAFYAAYAPPPFALYGTATILILAFATRFLPV
                     GFANADAAIRAINPEMEEAVRILGGGRLTAIRQVLAPLMKRNLIGAWLLVFIPATREL
                     SSAMFLYGPKTRTMSVMLMDMSEEGNFENLAALGFLLLASTLIIVGLATALLGRDFML
                     RRERQ"
     gene            10559..>11258
                     /locus_tag="VGC82_08335"
     CDS             10559..>11258
                     /locus_tag="VGC82_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0423315.1"
                     /translation="MTESKLSLIGLGKNFGSTPAVIDFSLSLHPGEFVSLLGPSGCGK
                     TTTLRMIAGFMKPSSGRIEVNGQTVSSPGKVVAPERRGMSMIFQSYAIWPNMTVGQNV
                     AFGLKLRKISTTEVTERVNRILDTVQLRHLKDRYPAELSGGQQQRVALARAIVIEPEV
                     LLLDEPLSNLDANLREEMRSEIRRLHDAFKITTVYVTHDQAEAMVTSDRIVVMNHGRI
                     EQVDDPVTMYRRPRT"
BASE COUNT         1887 a         3495 c         3648 g         2228 t
ORIGIN      
        1 atcggcgaac ctgacaggaa aaagttaacg aggtgttaag ctggcctgcc acggcagagg
       61 attggtcaaa taagtgcctg ggtggggcga tgtcgtaagc atccccgcag cgattgctgc
      121 cagcgccgcg ggataaatcg cgtgctcttg gcggagcaca cggttggcca gttcgtcctc
      181 ggtgtcacca ggcagcaccg gcaccgcggc ctgggctagg attggcccgg catccatctc
      241 ctgcgtcaca agatgcacgg tgcaaccgtg caacttcacg cccgccgcca atgcgcgggc
      301 gtgggtgttc aagccaggaa acgacggtag caggcttgga tggatgttca gcatccgtcc
      361 ttcccactcg ttgaccagga acggcgttag caaccgcatg tagcccgcaa ggcagacgac
      421 ttcgacgttc gccgctcgca gtgccgcatc gatggcggct tcgtgcgctg cgcggtcgcc
      481 actgaacggt cggtggtcga tgcacgcggc gggaactccg gcggcgcggg cggccgtcag
      541 cccagcggca tccgggcggt tcgacagtac gcaggcgatt tcggccggaa acgcaggatc
      601 gcgggccgcc gcgatcaatg ccagcatgtt ggacccacgg ccgctgatca ggatcgccgc
      661 gcggcgtttc acgccagcca accggcaggc ggatcgatcc gcacggacgc ggggccgtca
      721 gccgcctcga tcgcgccgat ccggctgacg gtttcgccct cggcttccag cagcagaccg
      781 gccgcctcgg ggtcggacac ggcaactacc atcccgacgc cgcagttgaa tacccgcagc
      841 atctcgtggc ccccgacccc gccggtgcgc gcgagccagt ggaaaacagg cggcacaggc
      901 catgcaggct gcagcgccgc catggtgttt tcggggagta cccgcagcag gttgccggga
      961 aggccgccgc cggtgatatg agccgccgcc ttcagcaatc ccgcacggtg cagcgcgagc
     1021 aacgaccgta catagatccg ggtcggcgtc atcaacgctt cgccgagtgt ctggccaggc
     1081 gcgaacggcg cggcgtcgtt ccagccaagg ccactggcgc gcacgatccg gcggaccagt
     1141 gaatacccgt tggaatgaac gcctgaactg gccaaaccga gcagatgatc gccggctgtg
     1201 agggggcgag gcagcaggtt ggctcgctcc gccgccccga ctgcgaagcc cgccagatca
     1261 tagtcgccct cggcatacat gcccggcatc tcagcggttt cgccgccgac tagggcgcag
     1321 cccgccagac ggcaaccctc ggcgatccct gcgatgacgg cacttgcgtg ggctaccgac
     1381 aattttccgg tggcaaagta gtccagaaag aacagcggtt cggcgccctg cacgaccagg
     1441 tcattgacgc acatggccac gaggtcgatg ccgacggtcc cgtgcaaagc ggtttcaacg
     1501 gcaatcttca gcttggtccc gacgccgtcg gtggtggaaa ccagcacggg atcgctaaag
     1561 cccgctgcct tcaggtcgaa caatgcgccg aaaccgccca ccccccccat cacgccggca
     1621 cgcgtcgtag ccttggcaag cggcttgata gcctccacca gcgcgtcgcc tgcctcgata
     1681 tccacaccgg cggcacgata atccagcctg ccggtatcgt ttaggaccgg gcgcgcgcct
     1741 tggttatcgg cagtggtcat ggcaacctct atattctaag ccgatccggt gaatgaaggc
     1801 agataggaac catgagcaag ctagcgactg caatccaccg gatcgaggag gcgccgcatg
     1861 cctgatggac caccggcgag aggaacgacc gcatccctgt tgaacctgcc caacatcgtt
     1921 accttcggcc gcctttgtgc ggtgcccctg gcgttttggc tcgtgcttgt ccaccgggtc
     1981 gaccaagcct ttctcctgtt cgtcgccgcc ggcctgtccg atgcactgga tggctggctc
     2041 gcccgacgct atggtggcaa cgcgatcggc gcgatgatgg atccggtggc ggacaaggcg
     2101 ctgctggtga caatgtatat caccctcgcc accatcagcg ccttgccggc gtggttggca
     2161 atcctggtgg tgttccgcga cctgctgatc gtcggcggaa tcgtcgtgct ggcggtcctg
     2221 ggacatgaag tagcgatccg tccgatatat atctccaagc tgaatacctt gatgcagatt
     2281 ttgttgatcg cggtcattct gctgcaagtt ggttttggcc tggggattcc gggtttggac
     2341 tttgtcctga cctggtgcgt ggcgacgacc acggtggcct cgggcgcggc ttatgtctgg
     2401 aacacagccc gaggaaaatg atgtgtcggc cgccggccag gatttactga gtcccccgaa
     2461 agaaccggcc gtcgccgcga aggaagcggc ggccgcgcca ccgtcggcgt tccatcatgc
     2521 gacacgaacg caacggtttg ctttgatcat cggcttggcg gtggtttcct ggctggcgct
     2581 ggcgctgttc gcctcgatcc tggcgccttt tgtcgccgcc ggcgtgctcg cctatgcgct
     2641 cgatccgccg acgacgcagt tgacgcgagt gggtctgccg cgcggggcgg cagcgctgtt
     2701 gatgatcgtc ggcgtgatcg ccgcggtgct gctgttcgca ttgctgctgt atccgctggt
     2761 gatcgtgcag atcgggctgc tggtgaaccg aattccgcaa tatgccaccc tcctgcaggg
     2821 ctgggcgcgg gaggtactca ccagtctgca ggacaatttc ggcccggatg tcgtcaacga
     2881 caaactgcgc gacgtggtca gcggccaggc gggatcgatg ctgagcatcc tgctgtccac
     2941 ggttacaggc ctgatcggag gcggcttcgc catcttcaac ctgctttccc tggccgtggt
     3001 aacgccggtc gtgggcttct atctgttgcg cgactggcca agcgtggtcc gcatgatcga
     3061 ttcgtggcta ccttaccgct accgggaggt aatccgggca caggcgcgcg aggtcgaccg
     3121 gatattgtcc gcctgggttc gcggccaggc gctatgctgt ctgctgttgg cactctatta
     3181 tgctgccggc ctgaccgtgg cagggctgga ccttggattg atcgttggac tcatggccgg
     3241 cctgctatcg ttcataccct atgtcgggtc gatcaccggt ggggtgacgg ccatcgggct
     3301 ggcgctggcg cagtttccgc agtggcgcgg cgtagcggtc atcggtgtcg tgctgatcat
     3361 cggccaaatc ctggagggct acgtgatcta cccgcgcttc ctcggcgacc gagtggaatt
     3421 accggcggtc tgggtgattt tcgccctgtt cgccggtggg gccgcgttcg gcttcctcgg
     3481 cgtgatgctg gcggtgcccg tggccgcgac gatcggcgtg ctcgcgcgct tctggctgcg
     3541 gcggtatctg gcgagcccgc tttacctgga cccgccctcg tcctgatggt gcgtcaaccc
     3601 ctgcaactgc cgctgccgtt ccagcacgaa ccacgttacg atgtgcgcga tttcatcccg
     3661 gcgcgttcga accaggacgc cttggcttgg ctggacaccg attggccgga gcagcggctg
     3721 gccttgttcg gaccgccagg gtgcgggaaa tcacacttgc ttcacatctg ggcggagcgc
     3781 tcaggggcgg tgcttctggc cggccagacg ctggccgatc tggacgggct gccgatgcga
     3841 gggtcattgg ccttggacga cgccgatacc gtagcggacg agacgctgtt gttccatctg
     3901 ctcaacactg ctcgcgatcg tggtctgcgc ctgctgctgt ccgcgggcgt ggcgccgtcg
     3961 cgctggcccg tccggctgca agacctgtcc agccggctac gcgcgatcac cgcgatcgag
     4021 attgggcaac ccggagatga cctgctggcc atgcttttgg tgcgactgct ggcggaccgg
     4081 caattagggg tgtcacaggc ggtacaggat tggctccttg tccacttgcc tcgatcgcct
     4141 tcggtgctgc gacttgccgt tgctcgtctg gatcgggcga gcctcgcatt cggttcagcg
     4201 attacccggc cgctcgcggc gcggatgttg tcggatctcg ttggcgcggt gccggacgac
     4261 gtttgcgtga cggagcggta gctatcgctc ggaccaagcc gtcgctcgga tgatcacgac
     4321 tggaactttt gatgacggag ttcgagtcgt cgtgccaaac gcacaggttc ctgtaggatg
     4381 tggcttcgtc tcgcgaagga attggatgga tggcgcataa ggccgatgct gcggatctca
     4441 tggaacgggt cgagacttcc ggcaaaaagc ccctggttgc gagaaccggc gcggcaaaaa
     4501 ccgcattcgg aaaagccgcc tcggagaaac ccggcacgcc gaagccggaa cgcccaccgg
     4561 agccgattgt cgatctggca gcgccggaac gctttataaa tcgcgagctg tcgtggctgg
     4621 acttcaacca ccgtgtgctg gaggaggctg aaaatcctcg gcatcccttg ctggaacggc
     4681 tgcgcttcct atcgatcagc gcgtcgaacc tggacgaatt ttactcagtg cgcgtggccg
     4741 ccctgatcgg tcaggccaag gcaggggcga catttacctc cgccgacggg cgcacggccg
     4801 cgcagcagct taccgccatc aagctgcggg cagagtcgct cctggttgac cagcagcgca
     4861 tcctgcggga gttgcttggc ctgctgcgcg atgccggggt ggtgctttgc acgaccgata
     4921 acttgtccga gaacgaaaag acctggctcg aagcctggtt catggagcgg gtgttcccgg
     4981 tggtaacgcc gcttgcggtc gatccggcgc atccgtttcc gttcatctcc aattttggcc
     5041 tcgtcctggc gctgcatctg gtgcgcgagg acgatggcca gggcatgcgc ggcttgttgc
     5101 cattgccgag ccagatcgat cgcttcgtac ggttgccggc agctgagtca gaggagtcgg
     5161 ttcgtttcat cctgttggac gatctggtcg tactattcct tggccgcgtg ttccccgggt
     5221 tccgaatgac cggccatggg ctgtttcggg taatccgaga caccgatgtg gaattcgagg
     5281 aggaagccga ggatctggtg cggtcatacg agactgcatt gaagcgccgg cggcgcgggg
     5341 tcgcgatcca cttggaaatc gctgccggga tgccgcctga actacgggag ttggtgatcg
     5401 acgagttgga cgccgcagcg gacgagatcc acgaggacca gcacttgctg ggcctggccg
     5461 acatgaagca gatgattgtc gatgaccgcc atgacctgct gttcgcacct tacacgccgc
     5521 ggtttccgga acgcatccgc gatttcggcg gcgattgctt cgcggcgatc cgggcaaagg
     5581 acatcatcgt tcaccatccc tacgagagct tcgacgtggt ggtgcagttc ctgcgtcagg
     5641 ccgcccagga cccggcagtg atcgcgatca agcagacgtt gtatcgcacc agccgcgaca
     5701 gcacgatcgt caacgccctt atcgaggcgg ctgaggccgg caaatcggtc accgctatgg
     5761 tggaactgcg cgcgcggttc gacgaggaag cgaacattcg gctttcacgc cgcatggaag
     5821 cggccggtgt gcaggtggtg tacggtttca ccggcctgaa aacacacgcc aagctgtcct
     5881 tggtggtgcg gcgggaagct ggcgctatac gatcgtatgc ccatttcggc accggaaatt
     5941 atcatcctat cacggcgcgc atctacaccg acctgagttt cttcaccacc gatcctgccc
     6001 tgacccgcga ttccgctcgg ttgttcaact tcatgaccgg ttacgcaagg ccggaagtca
     6061 tggaagaact cgggttcagc ccgctgacga cgcggacaaa actgctggcc ctgatccaca
     6121 gggaagccgc tttcgcccgt gacggtaagc cggcgacgat ctggctcaag atgaacagcc
     6181 tgaccgatgt ggagctgatc gacgcgctgt atgaagcctc gggcaccggt gtcaggatta
     6241 tggccgtgat ccgcgggata tgctgcctac gacctggcgt ggcgagacta tcggagaata
     6301 tccgagtcaa atcgatcgtc ggacgcttcc tggagcatgg ccgtatctat gccttcggca
     6361 acggccatcc cctccccagc cgccatgcga ggatattcat cagttccgcc gactggatgg
     6421 agcgaaacat ggattggcgc gttgaaacgc tggtgcccat tcacaatccc accgttcacg
     6481 cccaggtgct ggaccagatt atggtgatca atttgaaaga ttcgctgcag tcctgggaac
     6541 tgaatgccga aggtgcctgg aatcgcgtcg cgcccggccg gaagccgctt tcggcgcacg
     6601 agtatttcat gaccaacccc tccttgtccg ggcgaggatc ggcgctgcat gggccggcgg
     6661 tggcgtcccc ggtcgtggca ccccgccgcc aggacaggct gacgcaggac tagttatgct
     6721 attcgatcgg cctgtccgtt tgccgcgctg cggtgtcgtg gatctgggat ccaactccgt
     6781 ccgcttggtg gtttatgagg gtcacactcg caacccaacg ccgattttca acgaaaaggc
     6841 ggtgctgcgg ctgggccggg ggctgcagag caccggcagg ctgaacgagg agggcatggc
     6901 ccaggcgctg acggtgatgc accgctatca tgccgttgcc cgagccatgg atgccgaacc
     6961 gttcgaggtt ctggcgaccg ctgccgtgcg cgacgcggac aatggcccgg cgttcgtgca
     7021 ggcgttgcgc gaccggttgc cgggggtgcc gatcactatc ctgtccggcg tacaggaagc
     7081 ggcctactcg gccgatggca tgttgtgcgg gattccgacg gcggatggct tgctggccga
     7141 tatcggcggt gggtcgctgg aagttgtaag gctggttaac ggcacccgtg gcccgtctca
     7201 gaccctgaaa ctcggcgtga tccgtttggc ggagcgatca ggcggcgatc cgacacgggc
     7261 gcgggcgatt gcccaggcgg atctggaagc agtgccctgg ctgaccgagg cgtcgggccg
     7321 ggatctgtat ctggttggtg gcgcctggcg ggccttggcg cgcatccaca tggcgcagat
     7381 cgattatcct ctgagcatgg tccaccatta tgttatcggg cgagagcaag cccgcgacct
     7441 gaccgaactg atcgccggcg cgagcaaaaa ggtgttggag cggctgcctg gcgtgccgcg
     7501 ccgccgcatc gacgatctgc cgtttgccgc cgtggcgctg cggcgcctgc tgcgggtgac
     7561 caacgtaggc cgcgtggtgt ttagcgccag cggactgcgc gaaggctggt tcatgcggcg
     7621 aatgccccgg cagatccagg agcaggatcc gatgctaagc gccgcccagg agatggccgc
     7681 gcggtatggc cgcgacggag acctgccgcc gtcgctgctg gactggaccc ggcccctgtt
     7741 cgccgcagaa aatctggctt ccacgcagtt gcgggaggca atgtgctgga tgtccgatat
     7801 tggcagccat gaccatcccg agttccgggc cgaacaggca ttcctgcgcg tcttccgcca
     7861 gccgggcgtt ggactggatc atcccactcg ggccttcatg gcgctggcgg tggcactgcg
     7921 gtacgaggcg gagccggatg ctcccttcct gcgcccggct cggcttctgc tcgatgccgc
     7981 gaccgccgcc caagccgaag ttttaggaat cgccttgcgg ctggcttaca cactgtccgc
     8041 tgggacgaag gatctgctcg gcggcactga attgcggatc gaaggccctc ggctggtgct
     8101 tcggctgcgg accggcagtg gcgtgttcgc cggagaaagc gtgatccgcc gattggagcg
     8161 actggcccag gcgcttgggc tgcaggctgc cgcggaggag catctggcaa cgtgatccgt
     8221 cgggcacatg ccactcaggc aggcgcggtg cgcaaagtcg tactggcagc gtatcggcga
     8281 tatgttgcgg tgatcggaac agcgcctgcg ccaatgctgg acgattatgc gacgcggatc
     8341 gcgaccggtc aggtctggat atggcaagac gcggacgagg ttgccggtgt gctggtgctg
     8401 gagaacgggc cggattgttt cctgctgaat aacgttgcgg tgcggccgga ccgccagggg
     8461 caaggtatcg gccggtcgct gcttgatttt tccgaggccg aggcccgacg gtgcggttgg
     8521 gacgcgatca cgctctacac caacgcattg atggccgaga acatcgcgat ctatgcggcg
     8581 cggggatata tcgagcgggc acgccgaacg gagaacgggt tcgaccgggt ctatttggaa
     8641 aagcggctgg atggaaaagc gctggacgcc ggccgctagg atcgctgcgc gtaacgtgcc
     8701 tgccaagaga accgccgggt tgcagcccgc cggtctggtc aatcagtccg cgtacgcgta
     8761 gccttcgcac gggagaaaac gatgtttcga cgcaaacttt ttatggatca ttcgcggcag
     8821 cagggccgca attggcggcg cggcgcttga gcgcgactct cccgaaaatc cgccgccgca
     8881 acctgccgtt cgacccgctt gttattttgt gggtcgtact ggctgccgcg ttgaccctcc
     8941 tggtggtgaa cccgctgttc cgcctggtgc agacaagtct gcaggatacc gataccggcg
     9001 cgttcacctg gatgaactac gtcacggcct actccaggcc gcgctacatc accgcgatgc
     9061 tgaattcctt gcggctgggc gccggcgtta cggggttgtg cgtcctgttc gcggtcccga
     9121 tcgcctgggc ggtttcacgc accgacatgc cgggaaaggg catcgtccga ttcctcgtcc
     9181 tgggcgcctt cgtcacgcca ccttaccttg gcgcgatcgg ctggatcctg ttggcgggcc
     9241 cgaattcagg ctggctcaac cggatctgga tgtcgttgac cggcgcgcag gaaggcttgt
     9301 tcaacatcta ttcgttcgga ggcctgacct tcaacatcgc tatctactcg tttccttacc
     9361 tcttcatttt cactaccgcc gcgctcgatc tggtatcgtc ggaaatggaa gatgccgcca
     9421 acatcctcgg tgccggcatc ctgcgcacca cgctgcgggt cacgctgccg atggctctgc
     9481 cagcgattct cggcggtgcg atcgtgactt tcctggaagc gatcgccctt ttcggctccc
     9541 ccgccctgat cgccatccct gcgcgcttca atgtcgtcac gacccaattg ctgcaattct
     9601 tcagccagcc cgtcagagcg gaggtcgccg ccgcctatgc cgtgcccttg ttgttgatca
     9661 ccgtgctgct gttcggcttg cagcgcctgc tggtccgccg ccgcggcttc gtcgcgctga
     9721 ccggcaaggg cggcgagcgg cgggtcatgg agatcgggcc ctggcgctgg gtcatgctgg
     9781 gctacgcgct gttcattctc agcctggcgg tccttcttcc ttatatagtg ctcgggctgg
     9841 cggctctgtc gaaagcctgg ggcagaggta tgagcctcga taatttcacc ttgcacaatt
     9901 tcacttacct gctgttccaa catgagaccg ccgcccgctc cgtgctgaac agcttcacct
     9961 atgccgcggc cgcggcctgc atcgccatcg tgcttgggtt gtcgatcgcc tacatcgcca
    10021 gccgccggtt gttgccgttc gccggagtcc tcagctttct ctgcatggcg ccgttcgtcg
    10081 tgcccggcat cgtcctggcc attgccttct acgccgctta cgccccgcca cccttcgcgc
    10141 tgtatggcac cgctaccatc ctgatactgg cctttgccac gcgattcctg ccggtcggct
    10201 tcgccaatgc cgatgcggca ataagagcga tcaatccgga aatggaggag gccgtccgca
    10261 tcttgggtgg cgggcgtttg accgcgatcc gccaggttct tgcgccattg atgaagcgca
    10321 atctgatcgg cgcttggctg ctggtattca tccctgccac cagggagctg tccagcgcca
    10381 tgttcctgta tggcccgaag acccgaacca tgtccgtcat gctgatggac atgagcgagg
    10441 agggcaactt cgaaaacctt gctgcattgg gcttcctgct gctggcgtcc accctgatca
    10501 tcgtcggcct cgcgaccgcg ctgctgggcc gcgacttcat gctgcggcga gaacgacaat
    10561 gaccgaaagc aaattgtcgc tgatcggcct gggcaagaat ttcggctcta cccctgcggt
    10621 gatcgacttc tccctctcgc ttcaccccgg tgagttcgtg tcgctgctgg gtccctcggg
    10681 ctgtggcaag acgacgacgc tgcgcatgat cgccggtttt atgaagccca gttccgggcg
    10741 gatcgaggtc aacggccaaa ccgtgtcgtc ccccggcaag gtcgtagcgc cggagcggcg
    10801 cggcatgtcg atgattttcc aaagctacgc gatctggcca aacatgacgg ttggccagaa
    10861 cgtggcgttc ggcctgaaac tgcggaaaat ttcgacgacc gaggtgacgg aacgcgtcaa
    10921 tcgcattctc gataccgtgc agcttcggca cctgaaggac cgctatccgg ccgaactgtc
    10981 cggtggccag cagcagcgcg tggcgctggc ccgagcgatc gtgatcgaac cggaggtgct
    11041 gctgctggac gagccgctgt ccaacctgga cgccaatctg cgcgaggaaa tgcgcagcga
    11101 aattcgccgg ctgcacgacg ccttcaagat caccacggtc tacgtcacgc acgaccaggc
    11161 ggaagccatg gtcaccagcg accggatcgt cgtgatgaac catggccgca ttgagcaagt
    11221 ggacgacccc gtcacgatgt atcgccggcc caggaccc
//
LOCUS       DASWQN010000504         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1895393, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000504 DASWQN010000000
VERSION     DASWQN010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895393"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VGC82_08340"
     CDS             complement(<1..329)
                     /locus_tag="VGC82_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0423316.1"
                     /translation="MTTTTVARLLADSLEAHDVDQIFCVPGESYVGLTSVLSERNSIR
                     MIVCRHEGGAGFMAVADGRLRNRAGVCIVSRGPGLSNAMVSLHSAFHDATPMVMMVGQ
                     VERKDFGR"
     gene            433..1011
                     /locus_tag="VGC82_08345"
     CDS             433..1011
                     /locus_tag="VGC82_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003625105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEY0423317.1"
                     /translation="MLANLYARVMALAASPNAAWWLALIAFAESSFFPIPPDALLLPM
                     ALAKPRAAWRFAAICTAASVAGGALGYLIGFAVFDQVARPILDLYGYGPAFEAFQAKF
                     QEYGLWIILIKGLTPIPYKIVTIAAGAARFDFPMFMAASVLTRGVRFFLVATLLHFFG
                     DAVRDFVERRLTLVTSALAVGILGGFLALKFL"
     gene            complement(1615..2340)
                     /gene="otsB"
                     /locus_tag="VGC82_08350"
     CDS             complement(1615..2340)
                     /gene="otsB"
                     /locus_tag="VGC82_08350"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY0423318.1"
                     /translation="MDALPPFRDAALFLDMDGTLVDLAPTPDAVVVAPGLRDTLATLR
                     DSLDGALAIVTGRPIETVDRLFGNAAGAVAGEHGGAIRHRGDDPIERVNLPRPPAAWL
                     EAAEALVRSFPGALLERKARGFALHYRQAPEAREVFRAALRALVDPSPAFEVHPAHML
                     WEVRPLGADKGSAVVALMQRPPFAGRLPVFIGDDVTDEDGMAEARKRGGAGYRVDAVF
                     GDPEGVRSWLRRSAEAGGWATFP"
BASE COUNT          442 a          816 c          732 g          401 t
ORIGIN      
        1 cggccaaaat ccttgcgctc gacctggccc accatcatca ccatcggcgt tgcatcatgg
       61 aatgccgagt gcagcgacac catcgcgttg gacaatcccg gtccgcggga gacgatgcat
      121 acgccggcgc ggttgcgcag ccgcccgtcg gccactgcca tgaaccctgc cccgccctcg
      181 tgccggcaca cgatcatgcg gatcgagttc cgttcgctca gcacactggt caggccgaca
      241 tagctctcgc cggggacaca gaaaatctgg tccacatcat gagcttcgag gctgtccgcg
      301 agcaggcggg cgacggttgt ggtggtcatc ttggcgtaat ctccctatgt ggcgtcacga
      361 taggatggtg gtacggcagt gaccagagtg agccgccttc ggccacacca gccaccaccg
      421 aaaggggaca agatgctggc aaacttgtac gcgcgcgtga tggcgctcgc tgcgtccccg
      481 aacgctgcgt ggtggctggc actgatcgca ttcgccgaga gcagcttttt cccgatcccc
      541 ccggacgcac tgctgttgcc catggcgttg gcaaaaccgc gggcggcatg gcgcttcgcc
      601 gccatctgca ccgcggccag cgtcgccggc ggcgccctgg gctacctgat cggttttgcc
      661 gtcttcgacc aggtcgctcg gccgatcctg gatctttacg gctacggtcc ggccttcgag
      721 gcttttcagg cgaaattcca ggaatacggg ctgtggatca tcctgatcaa aggcctgacg
      781 ccaattcctt acaaaatcgt gacgatcgcc gcgggggcgg ctcggttcga tttccccatg
      841 ttcatggccg ccagcgtgct gacgaggggc gtgcgtttct tcctggtggc gacgctattg
      901 cacttcttcg gcgacgcggt gcgggatttc gtcgaacggc gattgaccct ggtcaccagc
      961 gccctggcgg ttggcatcct cggcgggttc ctggcgttga agtttctctg agcggccaac
     1021 acccggatac acagcaattt gcggggtgtc tcggcaacgc cgcggcctca tgcgccgttg
     1081 ccggcctatg gcaaaggcag atctacctta tacccagact tccagccgaa ccgcgggcgg
     1141 cgatcccgtc gagggcgccg atcccggtgg aacccggagc accaagcctg gtcaaaggga
     1201 caagtccgcg ccgaaggcaa ccgacccgaa gacgcggcag gacgtcacga ccggcgaccg
     1261 caatacccaa agagaatccc gcagcgctca gccgaaaagc ggttgacggt accggggttc
     1321 gcgccgctga tcctaccgga accctcatcc gcggttattc tgcctgagat ccactttcca
     1381 accggccttg gcctgatcag gtaaaggtct gctccagcgt ttgacaatcg tgtcatggcc
     1441 gcgctggcac ggccacctac gacttcgcct ggatacggga aagtcggcgc aagcggaaaa
     1501 ggcggcgcga cgtgggacca atccgacgct caggatgcga agccgactgc cacgttgccc
     1561 ggcagagtcc cctcgagccc gaagctgttt atcatcaacg ccaagctgaa acgcttaagg
     1621 gaaagtcgcc catccgcccg cttcggccga acgccggagc catgaacgga cgccctcggg
     1681 atcgccaaag accgcatcga cgcgataccc cgctccgccc cgtttccgcg cttcggccat
     1741 gccatcctca tcggtgacgt catcgccaat gaagaccggc agccgcccgg cgaagggcgg
     1801 gcgctgcatc agcgcaacca ccgcgctacc cttgtcggca cccagcggcc gaacctccca
     1861 cagcatgtgc gccgggtgca cctcgaacgc gggcgatggg tccaccaggg cccgcaacgc
     1921 ggcacgaaat acctccctgg cctccggcgc ctgccggtaa tgcagagcaa aaccgcgcgc
     1981 tttgcgttcc agcagcgccc cggggaaaga acgaaccagc gcttccgccg cttccagcca
     2041 tgcagcgggc gggcggggca ggttaacccg ctcgattgga tcatcgccgc ggtggcgaat
     2101 cgctccgcca tgttcgccgg cgaccgcacc ggcagcattc ccgaacaacc ggtccaccgt
     2161 ctcgatcggc cgtccggtca cgatggccag cgccccatcc aacgagtcgc gcagcgtcgc
     2221 gagtgtgtca cgcaatcccg gggcgacgac caccgcgtca ggcgtaggcg ccagatcgac
     2281 cagggtgccg tccatgtcca ggaacagggc ggcgtcgcgg aacggtggca gcgcgtccat
     2341 ggcctaagtg cagccactga cacgaaggtc gtaaactgga ttctgcatca t
//
LOCUS       DASWQN010000505         3685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1895683, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000505 DASWQN010000000
VERSION     DASWQN010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895683"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /gene="rpmC"
                     /locus_tag="VGC82_08355"
     CDS             complement(<1..216)
                     /gene="rpmC"
                     /locus_tag="VGC82_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY0423319.1"
                     /translation="MAKNALYKAGDVRAKTPDELSTLLLDLRKEQFNLRFQRATGQLE
                     NTSRVREVRKDIARLRTVQQQKLAGAKA"
     gene            complement(216..632)
                     /gene="rplP"
                     /locus_tag="VGC82_08360"
     CDS             complement(216..632)
                     /gene="rplP"
                     /locus_tag="VGC82_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008852401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY0423320.1"
                     /translation="MLSPKRTKYRKQHKGRIHGLSKGGTALNFGTFGLKALEPERITA
                     RQIESARRAITRAMKRAGRVWIRIFPDVPVSTKPAEVRMGSGKGSPEFWVARVAPGRI
                     MFEIDGVTPALAREALTLGAAKLPIKTKFIARVGDA"
     gene            complement(664..1341)
                     /gene="rpsC"
                     /locus_tag="VGC82_08365"
     CDS             complement(664..1341)
                     /gene="rpsC"
                     /locus_tag="VGC82_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY0423321.1"
                     /translation="MGHKVNPIGLRLGVNRTWDSRWFAGTDYAKLLHEDFKLRNHLRT
                     KLRGAGVSRVVIERPAKKPRVTIYAARPGVVIGKKGQDIEVLKRDLSKMAKAEVSLNI
                     VEIRKPEIDATLVAENIAQQLERRVAFRRAMKRAVQSAMRLGAQGIRINCGGRLGGAE
                     IARVEWYREGRVPLHTLRADIDYGTSTAKTTYGTCGVKVWVFKGEIMAHDPSAQDRKA
                     AEQAPQR"
     gene            complement(1343..1735)
                     /gene="rplV"
                     /locus_tag="VGC82_08370"
     CDS             complement(1343..1735)
                     /gene="rplV"
                     /locus_tag="VGC82_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY0423322.1"
                     /translation="MSKTSHVRSLPETEAQCVVSNLRISPRKLNLVAAMIRNLPAGRA
                     IATLTFSKRRIAGQVKKALESAIANAENNHSLDVDRLVVARAEVGRSIVMRRFHARGR
                     GRAARVEKWFSHLKIVVAERAQDEGGAA"
     gene            complement(1735..2013)
                     /gene="rpsS"
                     /locus_tag="VGC82_08375"
     CDS             complement(1735..2013)
                     /gene="rpsS"
                     /locus_tag="VGC82_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY0423323.1"
                     /translation="MARSVWKGPFVDGYLLAKAEAAKASSRNEIIKIWSRRSTILPQF
                     VGLTFGVYNGHKFLPVQVNENMVGHKFGEFSPTRTFSGHAADKKARRG"
     gene            complement(2015..2845)
                     /gene="rplB"
                     /locus_tag="VGC82_08380"
     CDS             complement(2015..2845)
                     /gene="rplB"
                     /locus_tag="VGC82_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY0423324.1"
                     /translation="MALRQFNPVTASLRGTVLINRSELWKGKPVKGLTEGQHSHGGRN
                     NHGRITTRFRGGGHKQSYRVVDFKRRKFDVPGVVERLEYDPNRTAFIALVKYQDNELA
                     YILAPQRLKVGDQVIAGARVDIKPGNAMPLAAIPVGTIVHNIEMKLGAGGKIARSAGT
                     FAQLVGKDAGYAQIKLMSGELRVVRSECFASIGAVSNPDNMNQKLGKAGRTRWLGFRP
                     HNRGVVMNPVDHPLGGGEGRSSGGRHPVSPWGQPAKGYKTRGNKRTDSLIIRRRNKGK
                     "
     gene            complement(2858..3178)
                     /locus_tag="VGC82_08385"
     CDS             complement(2858..3178)
                     /locus_tag="VGC82_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY0423325.1"
                     /translation="MTVAPKKPLSKEAMYNIIRAPLITEKMTLLSEKNQIGFKVALSA
                     SKPEIAAAIESLFNVKVTSVNTLIQKGKTKRFKGRPGVRSDVKKAIVTLAPGQSIDFT
                     TSLA"
     gene            complement(3175..>3685)
                     /gene="rplD"
                     /locus_tag="VGC82_08390"
     CDS             complement(3175..>3685)
                     /gene="rplD"
                     /locus_tag="VGC82_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY0423326.1"
                     /translation="NHKVKGVSDVSGTTRKPYKQKGTGNARHGSLRSPQFRTGGIVHG
                     PVVRSHEYSLNKKVRRLGLISALSLKLAENKLVVLDAATSAPKTGELAKKVKALGWKS
                     ALIIDGAVDEGFMRACRNIHRLDVLPTIGANVYDILNHDVLAITTAGLEGLKARINGA
                     AAATTGEAA"
BASE COUNT          576 a         1210 c         1147 g          752 t
ORIGIN      
        1 agccttggcg ccagcgagct tctgctgctg caccgtgcgc aggcgcgcga tgtccttgcg
       61 cacctcacgc acccgcgatg tgttctcgag ctggccggtg gcccgctgga agcgcagatt
      121 gaactgctcc ttccgaaggt ccagcaacag ggtggacagc tcgtccggcg tcttcgcccg
      181 gacgtcgccg gccttgtaga gggcattctt ggccatcagg cgtctccgac ccgggcgatg
      241 aacttcgtct tgatcggcag cttcgccgca cccagggtca gcgcctccct ggccagggcc
      301 ggcgtcacgc cgtcgatttc aaacatgatg cggccagggg caacccgcgc cacccagaac
      361 tccggcgagc ccttgccgga gcccatgcgg acctcggccg gcttggtgga gacaggcaca
      421 tccggaaaga tgcgtatcca gacacgcccg gcgcgcttca tcgcgcgggt gatcgcccgc
      481 cgggcggatt cgatctgccg cgcggtgatc cgctcaggct ccagcgcctt gaggccgaac
      541 gtgccgaagt tcagcgcggt gccgcccttg gacaggccat ggatgcggcc tttgtgctgc
      601 ttgcggtatt tagtgcgctt cggagagagc attttgcctt accaatcaat ctaccgttgc
      661 cggttaccgc tgaggcgcct gttcggcggc cttgcgatcc tgggccgacg ggtcgtgggc
      721 catgatctcg cccttgaaca cccacacttt cacgccgcag gtgccatagg tcgttttcgc
      781 cgtggacgtg ccgtagtcga tgtcggcgcg cagcgtgtgc agcggaacgc gaccttcgcg
      841 gtaccattcc acccgcgcga tttcagcccc gcccaaccgg ccgccgcagt tgatccggat
      901 gccctgggcg cccagacgca tggccgactg caccgcgcgc ttcatcgccc ggcgaaacgc
      961 cacgcgacgt tcaagctgct gggcgatgtt ttcggccacc agcgtcgcat cgatctccgg
     1021 cttgcggatt tcgacgatgt tcagcgacac ctcggccttg gccatcttgc tgagatccct
     1081 cttcagaacc tcgatgtcct ggcccttctt gccgatcacc acacccggcc tggcggcata
     1141 gatcgtgacg cgcggcttct tcgccggacg ttcgatcacc acccgagaca caccggcgcc
     1201 gcgcaacttg gtgcgcaggt ggttccgcag tttgaagtcc tcgtgcagca gcttggcgta
     1261 atcggtgccg gcgaaccagc ggctgtccca ggtgcggttc acgccaaggc gcaaaccgat
     1321 cggattgact ttgtgaccca tgttacgccg ctcccccttc gtcctgcgca cgttcggcga
     1381 cgacgatctt cagatggctg aaccatttct cgacccgagc ggcacgaccg cggccacgcg
     1441 cgtggaagcg gcgcatgacg atcgagcggc cgacttcggc gcgcgccacg accaggcggt
     1501 ccacgtcgag cgaatggttg ttctcggcgt tggcgatggc gctttccaac gccttcttta
     1561 cctgcccggc gatgcggcgc ttgctgaacg tcagcgtcgc gatggctcgg ccagccggca
     1621 ggttgcggat catcgcggcg accaggttca gtttacgcgg actgatccgc agattgctga
     1681 cgacacactg cgcctcggtc tcgggcaggc tgcggacatg agatgtcttg ctcattagcc
     1741 gcgcctcgcc ttcttgtcgg cggcgtgacc gctgaaggtg cgggtcgggg agaactcgcc
     1801 gaacttgtgg ccgaccatgt tctcgttcac ctgcaccggc aagaacttgt gcccgttata
     1861 gaccccaaac gtcaggccaa cgaactgcgg caggatggtc gaacggcgcg accagatctt
     1921 gatgatctcg ttgcgcgacg acgctttcgc cgcttcggcc ttggccagca ggtaaccgtc
     1981 cacgaacggt cccttccata cggaacgcgc catattactt acccttgttc cggcggcgga
     2041 tgatcaggct gtccgtccgc ttgttgccgc gcgtcttgta gcccttcgcc ggctgacccc
     2101 atggggacac cggatgacga ccgcccgaac tgcggccctc gccaccgccc agcggatgat
     2161 ccaccgggtt catgacgacg ccgcggttgt gcggcctgaa acccagccaa cgggtgcggc
     2221 cggccttgcc gagcttctgg ttcatgttgt ccgggttgga caccgcgccg atcgaggcga
     2281 agcattccga gcgaacgacc cgaagctcac cggacatcag cttgatctgg gcgtatccgg
     2341 cgtccttgcc gaccaattgc gcgaaggtgc cggctgaacg ggcgatcttg ccgccggcgc
     2401 ccagcttcat ctcgatattg tggacgatgg tgccgaccgg gatcgccgcc aaaggcatcg
     2461 cgttgcccgg cttgatatcg acccgcgcgc cggcaatcac ctgatcgccc accttcaggc
     2521 gctgcggcgc gaggatatag gccagctcgt tgtcctggta tttgactagc gcgatgaagg
     2581 ccgtccggtt cggatcgtat tccagccgtt ccaccacgcc cggaacgtcg aacttgcggc
     2641 gcttgaagtc caccacgcgg tatgactgct tgtgcccgcc gccgcggaac cttgtggtga
     2701 tgcggccgtg gttgttgcgt ccgccatggc tgtgctgacc ctcggtcagc cccttgacgg
     2761 gtttgccctt ccacagttcg gagcggttga tcagcacggt gccgcggagg ctggccgtga
     2821 ccggattgaa ttgtctgagt gccatcttcc gcgagcctta tgcgaggctg gtggtgaaat
     2881 cgatcgactg gccgggcgcc agcgtcacga tggctttctt gacgtccgag cgtacgcccg
     2941 ggcgtccttt aaagcgcttg gttttgcctt tttggatcag cgtgttcacc gacgtgacct
     3001 tcacgttgaa caagctttcg atcgccgcgg cgatttccgg cttgctggcg ctcaaggcga
     3061 ccttgaagcc aatctggttc ttctcggaca gaagggtcat cttctcggtg atcagaggag
     3121 cccgaatgat gttgtacatc gcctctttgg aaagcggctt cttcggtgcg acggtcatgc
     3181 ggcctctccg gtcgtggcgg cagcggcgcc attgatgcgg gccttcagac cctcgagccc
     3241 ggcggtggtg atcgccagaa cgtcgtggtt caggatgtcg taaacattgg cgccaatcgt
     3301 cggcagcacg tccagacggt gaatgttgcg gcaggcgcgc ataaagcctt cgtccaccgc
     3361 gccgtcgatg atcagcgccg atttccagcc caaagccttg accttcttcg ccagttcccc
     3421 ggttttcggc gcgctcgtgg cggcgtccag gactaccagc ttgttctcgg ccagcttcag
     3481 cgacaacgcc gaaatcaagc cgaggcggcg gaccttcttg ttcaggctgt attcgtgcga
     3541 gcggactacc ggtccatgca cgatgccgcc ggtgcgaaac tgcggcgaac gcaggctgcc
     3601 atggcgggcg ttgccggtgc ccttctgctt gtacggcttc ctggtcgtgc cggaaacgtc
     3661 cgagacgccc ttgaccttgt ggttg
//
LOCUS       DASWQN010000506         4579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_707070, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000506 DASWQN010000000
VERSION     DASWQN010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707070"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1178
                     /locus_tag="VGC82_08395"
     CDS             114..1178
                     /locus_tag="VGC82_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY0423327.1"
                     /translation="MPASYANHRAQLKAILLGWGMPEQNAETTAEILSWADLHGVDSH
                     GISMIPGYDALRRSGRARMDVRPEITRETPVSAVVDGGGGLGHVPAHFAMSTAITKAK
                     TAGMGAAVVRGSAHFGAAGFYSLMAAKQGLIGMSCTSASGIQVAPTFGKQARLGTDPW
                     SFAAPSADGVPFLLDMATTTVAAGRIRNKANENLPTPDGWVLDKEGNPSNDPLIAKEK
                     GGFLTSLGGSPENSSYKGYGLAVMVNVLASCLSGASLITDPMHTKKPGGMDIGHFFMA
                     IDPGIFRESDDFQSDVAAFMGALRATTPVDPARPVMVAGDPQWKYAETRMAEGIPVGP
                     GLLNQVRQIAQAAAAPWLLD"
     gene            complement(1584..2849)
                     /locus_tag="VGC82_08400"
     CDS             complement(1584..2849)
                     /locus_tag="VGC82_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0423328.1"
                     /translation="MNAPVHVDPTHSVDRSGPAYSLPLEKFDVSDPQLYQDDAWHPYF
                     ERLRREDPIHYVPASPYGPYWAVSRYKDIMHCEVHHEVFSNEIGGIQIEDQPKDMQRP
                     SFIRMDPPRHDDQRKVASPIVAPNNLANMEGLIRERTRMVLDALPRGEEFDWVEEVSI
                     KLTTMMLATLFDFPFEERAKLTYWSDVAICNVNAPDAPVHSEAERFEVLKGMAEYIGR
                     LFNERRGQPPKFDLLSMLAHGAATRDMPLKEFMGNLALLIVGGNDTTRNSMSGGLLAL
                     NQFPAEYAKLQANPGLVDSAVSEIIRWQTPVIHMRRTASQDTELGGKPIKAGDKVVMW
                     YISGNRDPDAIENPDSFIIDRTRPRQHLSFGFGIHRCVGNRLAELQLKILWQEILTRF
                     PKIEVVGEPRRVYSNFIRGIRSLPVRIPA"
     gene            2958..4205
                     /locus_tag="VGC82_08405"
     CDS             2958..4205
                     /locus_tag="VGC82_08405"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY0423329.1"
                     /translation="MEAIQHLKAAAAAARAAGHILAHSNAAVRNAALSGMADALRDGM
                     RDILAANEADVTAYHGSAAFLDRLTLTEARVEAMAAGLEDVAALPDPLDRTLSEWTRP
                     NGLRIQRIATPIGVIGMIYESRPNVGADASALCLKSGNAVILRGGSDSLHSARAINLA
                     LRQGLAASGLPEACIQVAPDADRSYVAAMLAGAGLLDLIIPRGGRSLVERVQREARVP
                     VLAHAEGLNHTYIHAAADPAMARRIVANAKMRRTGVCGATETLLIDRSVAAALLPLLV
                     EDLKTLGCDFRADAAARAVVPWLPAAAGADFDTEWLDSILSIAVVDGVDAALTHIAAH
                     GSEHTDAIVTEDAAVAEKFLRGVDSAVALWNASTQFCDGGEFGFGAEIGIATGRIHAR
                     GPVGLEQLTTYRYLVFGSGQVRA"
     gene            4222..>4579
                     /locus_tag="VGC82_08410"
     CDS             4222..>4579
                     /locus_tag="VGC82_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinic acid mononucleotide
                     adenylyltransferase"
                     /protein_id="HEY0423330.1"
                     /translation="MPDRIEPVPKFGDRRRTRVGLLGGSFNPAHEGHRHVAELALRRL
                     RLDQVWLLVSPGNPLKSTAGMASFADRLASAARIGDGRRVVASGIEALFGTRFTVDTM
                     RLLLRRFPLVRFVWIMG"
BASE COUNT          772 a         1377 c         1558 g          872 t
ORIGIN      
        1 gttgtcagcc atattcgttt ccctccgcat tgccggtctt cggccgctac ccttgccgga
       61 acatccggga cgcgcaaggt ttgcggccaa cgtcagcttg aggggaatca tccatgcccg
      121 catcctatgc caatcatcgc gctcagctta aggccattct cctgggttgg ggcatgccgg
      181 agcagaatgc cgagacgacg gcggaaatcc tgtcctgggc cgacctgcac ggcgtggaca
      241 gccatgggat ctcgatgatc cccggctatg acgcgctgcg gcgttcggga cgggcgagga
      301 tggatgttcg cccggagatc acgcgggaga ctccggtatc ggcggtggtg gatgggggtg
      361 gcggtcttgg gcatgtgccc gcgcatttcg ccatgtccac ggcgatcacg aaagccaaga
      421 ccgccggcat gggcgccgcc gtggtgcgcg ggtccgccca tttcggcgcc gctggctttt
      481 attcgctgat ggcggcgaaa caggggctga tcgggatgtc ctgcacatcc gcgtccggca
      541 tccaggtcgc accgacattc ggcaagcagg cgcggctggg gaccgatccg tggtctttcg
      601 ccgccccaag cgcggatggc gtgccgttcc tgctggatat ggccaccacc acggtcgccg
      661 ccgggcggat acgcaacaag gcgaacgaga acctgccgac gccggatggc tgggttctgg
      721 acaaagaggg caatccttcc aatgacccgc tgatcgcaaa ggaaaagggc ggcttcctga
      781 cctcgcttgg agggtcgccg gagaattcca gctacaaagg atatgggctg gcggtaatgg
      841 tgaacgttct cgcatcctgc ctgtcggggg cctcgctgat caccgatccg atgcacacga
      901 agaaaccggg cgggatggat atcggtcatt tcttcatggc gatcgacccc ggtatcttcc
      961 gtgaatcgga cgattttcaa agcgacgtcg ccgccttcat gggtgctctt agggcgacca
     1021 cgccggtcga tccggctcgc ccggtcatgg tggcgggtga tccgcagtgg aaatacgccg
     1081 agacgcggat ggcggagggg ataccggttg ggcctgggtt gctgaaccag gtgcgccaga
     1141 tcgcccaggc tgcggcggca ccttggctgc tggattgatg gattcttgtc ttgggccggg
     1201 ttggcggtgg acgggtttag tttcgaagct cgtgcggagg ctggctgcgg ctttttaaca
     1261 cggagttcac cgaaggggac ggagagcacg gagaatcgcg cattgctgtt ggcgatcggg
     1321 agctcagagt ggtttcacgg tgactggaaa cagagttctc tcgtggttgc gaagaaagtc
     1381 gtgggtggcc gcgccaggca cggccatgac acataggcgt caggcggcaa actggctcgc
     1441 acctttttca gtcagtgttg aaccgctctg gctttgggac cgctcgacct tccgtcgcac
     1501 gcgctgtcca ccttcaacga tgaatggaac aaaagctggc attgccggtc attacgtgtg
     1561 cgggtcgtcg gacgtccaac gcctcaggct gggatacgta ctggcagcga acggattccg
     1621 cggatgaagt tggagtagac gcggcgcggt tccccaacca cctcgatctt cgggaagcgc
     1681 gtcaggatct cctgccacag gatcttcaac tgcagttcgg ccagccggtt gccgacacag
     1741 cggtggatgc cgaaaccgaa cgacagatgc tgccttgggc gcgttcggtc gatgatgaag
     1801 ctgtccgggt tctcgatcgc gtccggatcg cgattgccgg aaatatacca catcaccact
     1861 ttgtcgccgg ccttgatcgg cttgccaccg agctcggtgt cctgcgacgc cgtgcggcgc
     1921 atgtggatca ctggggtttg ccagcggatg atctccgaca cggcgctgtc caccagcccc
     1981 ggattggcct gcagcttggc gtattcggcc gggaactggt tcaacgccag cagcccgccg
     2041 ctcatggagt tccgcgtcgt gtcgttgccg cccacgatca gcagtgccag gttgcccatg
     2101 aactccttca gcggcatatc ccgggtggcg gcaccgtggg cgagcatcga caacaggtcg
     2161 aactttggcg gctgaccgcg ccgctcgttg aacagccggc cgatgtattc cgccatgcct
     2221 ttcaggactt cgaaccgttc cgcctcggaa tgaaccggcg cgtccggcgc gttgacgttg
     2281 cagatcgcca catcggacca gtatgtcagt ttagcccgct cctcgaacgg gaagtcgaac
     2341 aacgtcgcca gcatcatcgt ggtcagcttg atcgagacct cctcgaccca atcgaattct
     2401 tcgccacgcg gcagggcgtc caggaccatg cgggtgcgct cgcggatcag cccttccatg
     2461 ttcgcgaggt tgttcggcgc gacgatcggg ctggcgacct tgcgctggtc gtcgtggcgc
     2521 ggcgggtcca tgcggatgaa gctgggacgc tgcatgtcct tcggctgatc ctcgatctgg
     2581 atgccgccaa tctcgttgga gaagacctcg tggtgcactt cgcagtgcat gatgtccttg
     2641 tagcgggaga ccgcccagta cgggccgtac gggctggcgg ggacgtagtg gattggatcc
     2701 tcccgccgca aacgttcgaa gtagggatgc caggcgtcat cctgataaag ctggggatcg
     2761 gatacgtcga acttctctag cggcagcgag taggctgggc cgctccggtc aaccgaatgt
     2821 gtcggatcga catgcacggg tgcgttcatt gttagccctc cctttgtcag ggaaagcagc
     2881 acgccgacca tggcaccgtc aacggcaacg tcgaaccggg actggaaacc tgacgctcga
     2941 ttaggcacat tggcgagatg gaagcgatcc aacatcttaa ggccgccgcc gccgcggcca
     3001 gggcggccgg ccatatcctc gcgcacagca acgcggcagt gcgaaacgca gcgctttccg
     3061 gcatggcgga tgcgttgcgc gacgggatgc gagacatcct ggccgccaac gaagcggacg
     3121 tgacggccta tcatggttca gcggcgtttc tggaccggct aaccttaacc gaggctcggg
     3181 tcgaggccat ggccgcggga ctggaagacg tcgccgcact ccccgacccg cttgaccgca
     3241 cgctcagcga gtggacaagg ccaaacggac tgcggatcca gcggatcgcg accccgatcg
     3301 gcgtgatcgg catgatctac gaaagccggc cgaacgttgg cgcggatgcc tccgcattgt
     3361 gcctgaagtc gggcaatgcg gtgatcctgc gcggcggatc ggacagcctg cacagcgccc
     3421 gggccatcaa cctggcactt cgtcagggac tggcggcgtc cgggctgccg gaggcgtgca
     3481 tccaggtcgc gcccgacgct gaccggtctt atgtggccgc catgctggcg ggtgccggcc
     3541 tgctggacct gatcatcccc cgcggcggca gatccctggt ggagcgagtg cagcgcgagg
     3601 cccgggtgcc ggtcctggcg catgccgaag gtctgaacca cacctatatt cacgccgccg
     3661 ccgacccggc gatggctcgg cggatcgtgg cgaacgcgaa aatgcggcgc accggggtgt
     3721 gcggcgcgac ggaaacgttg ctgatcgatc gttccgttgc cgcggcgttg cttccgctgc
     3781 tggtcgaaga cctgaagacc ctgggctgcg acttccgggc ggacgcggcg gcgcgggcgg
     3841 tggtgccgtg gcttccggcg gcggccggtg ccgatttcga caccgaatgg ctggactcga
     3901 tcctgtcgat cgccgtggtg gatggcgtcg atgcggcgtt gacacacatt gccgcccatg
     3961 gcagcgaaca caccgacgcc atcgtcaccg aggacgcggc ggtcgcggag aagttcctgc
     4021 gcggcgtcga tagcgcggtg gcgctgtgga acgcctccac ccagttctgc gacggcggcg
     4081 aattcggctt tggtgccgaa atcggcatcg ccacgggacg tattcacgcg cgtgggccgg
     4141 ttggtctgga gcagttgacg acgtaccggt atctcgtgtt cggatcggga caggtgcggg
     4201 cctgagaacg tcttgccgtt aatgccggat cggattgaac cagtgccgaa gttcggcgac
     4261 cggcgacgaa cgcgcgtcgg gttgctaggc gggtcgttca atcccgcgca cgaaggccat
     4321 cgtcatgtgg cggaactggc gctgcggcgg ctgcggctgg atcaggtctg gctgctggtc
     4381 tcgccgggga atccgttgaa gtccaccgct ggcatggcct cgttcgccga tcggctggcg
     4441 agcgcagcac gcatcggcga tggtcgccgc gtggtcgcca gcgggatcga ggccctgttt
     4501 ggcacccggt ttaccgttga tacgatgcgg cttttgttgc gccggtttcc attggtgcgg
     4561 ttcgtctgga tcatggggg
//
LOCUS       DASWQN010000507         6189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_48448, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000507 DASWQN010000000
VERSION     DASWQN010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48448"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VGC82_08415"
     CDS             <1..361
                     /locus_tag="VGC82_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423331.1"
                     /translation="LLRYEGEQILPGDKTAPANARGLLLVGMTPGKEVEAAFNVWYDT
                     EHVPALASVPGVLCARRFRTSGTGPKYMALYHLASPEVIATPAWKDASGSTPMPQRVR
                     DQISDRLRLVCRNYVRG"
     gene            441..1643
                     /locus_tag="VGC82_08420"
     CDS             441..1643
                     /locus_tag="VGC82_08420"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY0423332.1"
                     /translation="MQDIPLNKCVLDKRNVLRAPFVNRQPADLPKAEAEYYAHARTMM
                     GRSAMGKKIAIVGVGAVGGYTGAHMAREGENVTFIDPWPEHIEEIKRNGLRITHHQGE
                     EPFTVQPRALHLTEVQQVSKESPFDIAFICMKSYDTAWATRLIRQYLAPNAYVVSLQN
                     CMNEATIADVVGWGRVLGCIASNISVGLIEPGHIHRGGLKGGAAHTVYRTGEVHGGMT
                     GRVNEVCCLVSHADSAKVTDNLWGERWSKLVTNAMGNGVSACTGLSGAQMAQNETVRR
                     FQARLASEAIRIGQALGYRLEEINHLPPEVIAGAGEGDPAALARYEERLRTQPRGSAT
                     QRPSMGQDMMKGRRTEIEFLNGYVVREGEKIGIAARANQRLVELVTKVERGELRPDPR
                     HITELRLN"
     gene            1775..2335
                     /locus_tag="VGC82_08425"
     CDS             1775..2335
                     /locus_tag="VGC82_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423333.1"
                     /translation="MSGSPTFGRYTEIPYEQMTPEQQEGYRAMIESRGGLPGPSKIWV
                     HNPKLAKVAGPFGGHFHAGHYSLTEREREIAVCVITSKLHSAYPTNAHERRGKEVGLP
                     ADKVEAIITGLQTSFADEREQVVYEMAVALANARWVPQGLYDRAVKALDHTGITDVIT
                     LMGHYSSVAMTLAFYDVPAGAVGMAR"
     gene            complement(2909..3802)
                     /locus_tag="VGC82_08430"
     CDS             complement(2909..3802)
                     /locus_tag="VGC82_08430"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEY0423334.1"
                     /translation="MWEEYNMTQLGTGEFTYELVRDFLRLPNGEAFGLVSRVAADSQD
                     RVYVFQRRDPPVVVFDRDGRYLGAWGSGEVKDPHGLKIVNDVVYTTDRSDSVAKSFTL
                     DGKVLLVLGIPGVHSDTGCTGSPWLALRAAGPFNHPTEMIAHPNGDIYVTDGYRNARV
                     HRFTGDGRLVMSWGTPGKAAGEFHLPHSIAFDPDGKLYVADRANKRIQIFSPEGDFLG
                     MWTGMGGPNDVTRGRDGTFIIAEQEDGGNPAYVCVRDAKGTVLAKMESRHVHGVGVDS
                     RGDIYAGLTVDRSVDKFVRTG"
     gene            complement(3877..5493)
                     /locus_tag="VGC82_08435"
     CDS             complement(3877..5493)
                     /locus_tag="VGC82_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0423335.1"
                     /translation="MQADYIIIGAGSAGCVLANRLTEDPATKVLVIEAGGRDWNPLIH
                     VPAGFMKMLDHPTLTWKFRSEPDPGTNGRAIVYTRGRVIGGSSSINGLIYIRGQPEDY
                     DHWAQLGNRGWSWDDCLPFFRKAENWEGEGSAARGKDGPLFTSKMDRPAICATVIEAG
                     KQIGLEYCEDVNDLQPGAGNSIGWCQQTRGGRRRASAARTYLHPALKRPNLELVTNAL
                     VHRIGFDASKRATSVQFSRGGVIERAEARHEVILSAGAIGSPHILQLSGVGNPDHLGK
                     IGVPIVHGLPGVGRNMQDHYVARISYPVVGAQTANEKARGLPFAGEVMRWLMTGKGML
                     SYSPSIVAASVKVLESSATPDMQCTFAPGSFKDGQIGQLDDAPGLSAGAWQMRPFSRG
                     YVEARSNQPGDMPAINPRYLSDEMDQRAIVGGLRFARQIFAAPALKTFVREESLPGVH
                     LQADDELLDYARRNGSTCYHASCTCMMGLHPTSVVDNELRVHGIDGLRVIDASVMPAV
                     TSTNTNAPTIMIAEKGAAMIKGTARQKLAA"
BASE COUNT         1251 a         1884 c         1864 g         1190 t
ORIGIN      
        1 cctgctgcgt tatgaaggtg agcagatcct cccgggcgac aaaaccgcgc cggccaatgc
       61 acgcggactc ctgctggtcg gcatgacccc tggtaaggag gtcgaggcgg cgttcaacgt
      121 ttggtatgac actgagcatg taccggccct tgccagcgtt ccaggggtgc tgtgcgcacg
      181 tcgtttccga acgagcggaa ccggcccgaa atacatggcg ctctatcatc ttgcgtcgcc
      241 agaggtgatc gccacgccgg catggaaaga tgcgagcggg tcgacaccga tgccccagcg
      301 cgtaagggac caaatcagcg atcggctgcg ccttgtgtgc cgaaattacg tgcgcgggta
      361 gacggcgaca tcgatccgcc ggatccgcct ggcggatcga gccgtccagc gacgaacgta
      421 ctccgtctct tcggtaagag atgcaggaca ttccgttaaa caaatgcgtg ttagacaaac
      481 gtaacgttct gagggcgcca ttcgtcaacc gccagcctgc tgatttgccc aaagccgaag
      541 ctgagtatta cgctcatgcc agaacaatga tggggcggag tgccatgggc aagaagatcg
      601 ccattgtcgg cgtgggcgca gttggtggat ataccggcgc gcatatggcg cgtgaaggcg
      661 agaatgtcac ctttatcgat ccgtggcccg agcatatcga agagatcaag cggaacggcc
      721 tgcgcataac gcaccaccag ggcgaggaac cgttcaccgt acaaccccgc gcgctgcatc
      781 tcaccgaggt ccaacaggtg agtaaagaat cgccgttcga catcgccttc atctgtatga
      841 agtcctatga caccgcctgg gctactcggc ttattcgaca atacctggcg ccgaacgcct
      901 atgtcgtttc gttgcaaaac tgtatgaacg aggcgacgat cgccgatgtt gtcggctggg
      961 gcagggtgtt aggctgcatc gccagcaata tcagcgtcgg cctgatcgag cccggccata
     1021 tccatcgcgg cggccttaaa ggtggtgcag cgcacactgt ctaccgcacc ggcgaagtgc
     1081 acggcggcat gactggtcgc gtaaacgagg tgtgctgttt ggtttcccac gctgacagcg
     1141 caaaggttac ggacaatctt tggggcgaac gttggtcgaa gctggtcacc aacgcgatgg
     1201 gaaacggcgt ctccgcctgt accggtctct ctggtgcgca gatggcacag aacgaaactg
     1261 tccgccggtt ccaggctcgg ctcgccagcg aggccatccg tattggccag gcgttgggat
     1321 accgacttga ggaaatcaat cacctgccgc cagaggtaat cgccggcgcc ggtgagggcg
     1381 atcccgcagc gctcgcgcgc tacgaggaac ggttgcggac ccagccgcgc ggttcggcga
     1441 cgcaacggcc ttcgatgggg caggatatga tgaagggacg gcgcaccgag attgagtttc
     1501 tgaacggcta cgtcgtgcgc gaaggcgaaa aaattgggat tgcggcccgc gccaatcaac
     1561 ggctcgtgga acttgtaacc aaagtcgaac ggggtgagct gcggcctgat ccgcgtcaca
     1621 tcaccgagct tagattgaac tgagcacgac aaccggatgc acgagcaaac gtaaccgggc
     1681 cggactcggc gcgtcctcaa cgcgctcacg ttcgacaggg atccagggtt gtcgcaagat
     1741 gcgctgcgaa tgttcggaac ggagaaacaa aaaaatgtcc ggctcaccaa ccttcggccg
     1801 ttataccgag ataccctacg agcaaatgac gccggaacag caggaaggct atcgcgccat
     1861 gatcgagagc cgcggcgggc tccccgggcc gagcaagata tgggtgcaca atccgaagct
     1921 ggcgaaagtc gccgggccat tcggcggcca tttccacgcc gggcattatt ctcttacaga
     1981 gcgcgagcgc gaaatcgccg tctgtgtcat cacaagcaaa ttacattctg cctatccgac
     2041 caacgcccat gaacggcgcg gcaaggaagt cgggctgcct gccgataaag tcgaggccat
     2101 catcactggc ctgcagactt cgtttgcgga tgagcgtgag caggtcgtct acgagatggc
     2161 ggtcgccctt gccaatgcgc gctgggtgcc acagggcctt tacgaccgcg cagtcaaggc
     2221 actcgaccat accggtataa cggacgtgat caccttgatg ggccactaca gcagcgtcgc
     2281 gatgactctg gccttttacg atgtaccggc cggggcggtg ggcatggcgc gctgactgtg
     2341 gaagcgcctg aaaccgagca cgccagtccg cgctccttgg tcatcgtccc cgaactcgat
     2401 ccgggacaat gaccggtgga gttcctacgc tgcttccgtt gccgccatgg tggtgcgatg
     2461 ctgctgccgg cctcggtggg atcgaatggc cgtgaacggt gcatggccgc ctgactattc
     2521 catatcacca ggtcccgcta ccgtcgctct gagcgcgggg aaataactgg ctatgcacgg
     2581 acaccaggcc tcgcacgcgg ctttgagttc atcggtaagg cgctggtcgt ggaacaggat
     2641 cgcactggat tcgtccatgc ccttttcaat ctcagcgcct gtccctgatc agtggctcca
     2701 tgatgtcgat cctgacgttt gctcccaaag aacgggcgtg aaaccggagc ggtaatgtta
     2761 tggcaaacct cgctggcacg aatggtggcc aaatccgaga ccacggggcg tgagaagtat
     2821 gattcggatc tcaacatcgc cctcccgccg aggagcgtcc gttgatgcat cggaggctga
     2881 ctatgacaga cagcagttaa acggtcgctc agcctgttcg cacgaacttg tccacgctgc
     2941 ggtccacggt caatccagca taaatgtcac cgcgcgagtc cacgcccacg ccatggacat
     3001 ggcggctctc catcttcgca agaaccgttc ctttggcgtc acgtacacac acatacgcag
     3061 gatttccacc atcctcctgc tcggcgatga tgaacgtgcc atccctgccg cgggtgacgt
     3121 cgttcgggcc gcccatgccg gtccacatgc caaggaagtc gccctccggc gagaagatct
     3181 ggatgcgctt gttggcccga tcggccacat acagctttcc gtcaggatcg aacgcaatgc
     3241 tgtggggcaa atgaaactcg cccgcggcct ttcccggcgt gccccacgac atcaccagcc
     3301 ggccatcgcc agtaaaacgg tgcactcggg cgttgcgata gccgtcggtc acgtagatgt
     3361 cgccgttggg atgggcgatc atttcggtag gatggttgaa cggccccgcc gcgcgcagcg
     3421 cgagccatgg agagccggtg cagcccgtgt cagagtgcac gccagggatg ccgaggacca
     3481 gcaggacttt gccgtccagc gtgaatgatt tcgcgacaga gtcagagcga tcggtggtgt
     3541 agacgacgtc gttgacgatt ttcaggccat gcgggtcttt gacctcgccg ctgccccaag
     3601 cgcccaggta ccgtccgtcg cggtcgaaga ccaccaccgg gggatccctg cgctggaaga
     3661 cgtaaacgcg atcttgcgaa tcagccgcca cccggctgac cagcccgaat gcttcgccat
     3721 tgggcaaccg gagaaaatcg cgaaccagct cgtaagtaaa ttcgcctgta ccaagttggg
     3781 tcatgttgta ttcctcccac attgagtatg cgcacttaag ccgacatgtc cctgctgccc
     3841 ccgacgatcg accgcgacac gacaacgtcg actgcgctac gccgccaatt tctgccgagc
     3901 agtgcctttg atcatcgctg cgcccttctc ggcgatcatg atcgtcggcg cgttcgtatt
     3961 ggtcgaggtg actgccggca tcaccgacgc gtcgatgact cgcagcccat cgataccatg
     4021 cacacgcaat tcgttgtcaa cgacacttgt cgggtgcaat cccatcatgc aggtgcagct
     4081 cgcgtggtag caggtgctgc cgttgcgccg cgcgtagtcg agcaactcat cgtccgcctg
     4141 aagatgaacg ccgggcagac tttcttcccg gacgaacgtc ttcagcgcgg gtgcggcaaa
     4201 aatctgccgc gcaaatcgca gaccgccaac gatggcacgc tggtccattt catcagaaag
     4261 atagcgcggg ttgatcgccg gcatgtcgcc cggttggttc gatcgggcct ccacatagcc
     4321 gcgcgaaaac ggccgcattt gccatgcacc agcgctcaga ccgggtgcat cgtccaactg
     4381 accaatctgg ccgtccttga agctgcctgg tgcaaacgtg cattgcatat cgggcgtcgc
     4441 cgaactctcc agcaccttaa cggaggcggc gacgattgag gggctgtagc tcagcatgcc
     4501 tttgccggtc atcagccagc gcatgacctc gccggcaaac ggtaggccgc gggccttctc
     4561 gttggcggtc tgggcgccga cgactggata ggagatacgt gcgacataat ggtcctgcat
     4621 gttgcggccg acaccaggca atccatgcac gatcggaaca ccgatctttc caaggtgatc
     4681 gggattgcca acgcctgaca gttgcagaat gtgcggcgaa ccgattgcgc ccgccgacag
     4741 gatcacctcg tgccgagcct ctgcccgctc gatgaccccg ccacgcgaga actgaacgct
     4801 tgtcgcccgc ttgctggcgt cgaacccgat gcggtggacc agcgcgttgg tgacaagttc
     4861 gaggttcggc cgcttcagcg cgggatgcag ataggtgcgc gcggcgctgg ctcgacggcg
     4921 gccaccgcgg gtctgctggc accagccgat gctgttgccc gcgccgggct gcaggtcatt
     4981 gacgtcctcg cagtactcaa ggccgatctg tttgccggcc tcgatcaccg tggcgcagat
     5041 cgccggccgg tccatcttcg aggtgaacag cggcccgtcc ttgccacgtg ccgcgctgcc
     5101 ttcgccctcc cagttctcag ccttgcggaa gaacggcagg cagtcgtccc acgaccagcc
     5161 gcggttgccg agttgtgccc agtggtcgta atcctcaggt tggccgcgaa tgtagatcaa
     5221 cccgttgatc gaggaagagc ccccaatcac gcggccgcgc gtgtagacga tggctcttcc
     5281 gttggtaccg ggatcgggtt cggaccggaa cttccaggtc aaagtcgggt ggtcgagcat
     5341 cttcatgaac ccggccggga cgtgaatgag cgggttccag tcccgtccgc ctgcctcgat
     5401 caccaatact ttcgtggcgg gatcctcggt tagccggttg gccagcacgc agcccgctga
     5461 gcccgccccg atgatgatgt aatccgcctg catgtcctgc cccccatgat gcggtccaat
     5521 gtcggactgc ttgtttacac gttcactcta gcaaaacggg ggcgtaccga aagatgacgt
     5581 gaggggcgtg ccggctgagg tgtttcgtca tgttcagcta cgttgtgtca gagcataagt
     5641 gccggcgact ggatcaacct ttaagtcttc cggggtcgag ggagtaattt ttgagtcatg
     5701 gaacaaatgc cggcatgttc cattgaaacg gcagtcggat cgcagtctcc tgacctattt
     5761 agctaggcca agggcgccga caggcgttcg gcggcctcca gatccgctgt atccgagccc
     5821 tccaagaact acgcgaggac tcgccgcaga agcgcccgcg ccctcggctc gatccttgga
     5881 ggcgagcagc agcgttgcca gatcaaccgc cgtgcgaatt cccatgctgg cgctttgacg
     5941 accgctccat tcaagccact gcataaagca ctttcctgcg tcatcaatta gaagctgcgg
     6001 cgttgagaga attacgtctc tcttcagtcg cagcaactct gccatgtaac agagatctcc
     6061 gcttgcgtcg aaaatcgaac ggcttcgtca attaaatgcg gggcagagag cggcgccacc
     6121 aggggcggat attagccgaa atggaattac caggcctacc cgtgctaatt cgtccatttc
     6181 actggccgt
//
LOCUS       DASWQN010000508         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2027460, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000508 DASWQN010000000
VERSION     DASWQN010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027460"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VGC82_08440"
     CDS             <1..322
                     /locus_tag="VGC82_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY0423336.1"
                     /translation="DELGGHLVSGHVDGVGTALSATPENASIRWLFRVPVDISRFIAV
                     KGSVAVDGVSLTVNEVRGDTFGVNIIPHTASVTGFGRLLPGDPVNIEIDTLARYVARL
                     AETA"
     gene            322..1476
                     /gene="ribB"
                     /locus_tag="VGC82_08445"
     CDS             322..1476
                     /gene="ribB"
                     /locus_tag="VGC82_08445"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY0423337.1"
                     /translation="MENLHEKFVTSELSKYLSSAEELIEEARNGRMFILVDDEDRENE
                     GDLVIPGQFATPDAINFMARFARGLICLAMTRSRVEKLGLPLMSRQNGTRHQTAFTVS
                     IEAREGVTTGISAADRSKTIAVAINPDSTRDDITTPGHVFPLMAREGGTLVRAGHTEA
                     SVDIARKAGLAPAAVICEIMNDDGTMARLPDLVTFAQHHNLKLGTIADLIAHRRLTER
                     LVRKVEEGAYAHPAGGDWRAVVYANTVEYQEHLALVKGDVSGNEPVMVRMHAVDLLDD
                     MTGSPHWIALHNAMHMIGKAGRGVLVLIREHRATALAERVRLLAAGTPRPQRELRDYG
                     IGAQILLDLGIKNMILLTNRRRTVIGLEGYGLNIIEQRPIPNVVGVDYEG"
     gene            1473..1955
                     /gene="ribH"
                     /locus_tag="VGC82_08450"
     CDS             1473..1955
                     /gene="ribH"
                     /locus_tag="VGC82_08450"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY0423338.1"
                     /translation="MSTQDAELPQAPRVEGPSPHLLIVRAPYYRDVVDGLTTGANRIL
                     AEAGATAETLDVAGAYELPQAIRIALRGSRKFDGFVALGCIVKGETDHYDFICKATMD
                     GIMNVALQFGIALGTGLLTCDTVAQATARSNQDGHNKGAEAAAAALLQINVARRLGAA
                     "
     gene            1955..2431
                     /gene="nusB"
                     /locus_tag="VGC82_08455"
     CDS             1955..2431
                     /gene="nusB"
                     /locus_tag="VGC82_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY0423339.1"
                     /translation="MARPPKVRTRTASRVAAVQALFQAEQAGDNPETVIDQFVRHRLG
                     ELEGQYGFEDGRIPDAEVKLFSRVVREAVRRQDAIDPMLVEALPADWPLSRIDPVLRA
                     LMRAAAAELAMPDGPPAKVVINEYLDIARGFFTGPEPGLANAVLDRLARVLKTAEF"
     gene            complement(2473..>2759)
                     /locus_tag="VGC82_08460"
     CDS             complement(2473..>2759)
                     /locus_tag="VGC82_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423340.1"
                     /translation="ASRTLVLTDMVLNLETEKLPVLVRSVVRMFGSNAPDGMPPPYLR
                     FVVRMRREAALTAVRRMLALQPERAIFAHGDCFETDATQRLRHSFRWLLR"
BASE COUNT          522 a          872 c          893 g          472 t
ORIGIN      
        1 cgacgagttg ggtggccatc tggtatcggg tcacgtcgac ggggtcggta ccgcgctatc
       61 cgccactccg gaaaacgcct ccatccgctg gctttttcgt gttcccgtcg atatctcacg
      121 tttcattgcc gtgaagggca gcgtcgcggt ggatggcgta tcgttgactg tgaacgaggt
      181 gcggggcgat acatttgggg tgaacatcat tccccacact gcctcggtca cagggttcgg
      241 aaggctcctg ccgggggacc cggtcaatat cgaaatcgat accctcgcgc gctatgtcgc
      301 tcgcctggcg gagaccgcct gatggaaaac ctgcacgaaa aattcgttac cagcgaactg
      361 tccaaatatc tctcctccgc cgaggaactg attgaggaag cccgcaacgg tcgaatgttc
      421 atcctggtcg acgacgagga ccgcgagaac gagggcgacc tggtcatccc cgggcagttc
      481 gccacgccgg acgccatcaa cttcatggcc cgcttcgcgc gcggcctgat ctgcctggcg
      541 atgacccggt cgcgggtcga gaaactcggc ctgccgctca tgagccggca gaacgggacg
      601 cggcaccaga ccgcgttcac cgtctcgatc gaagctcgcg agggggtcac gaccggcatc
      661 tccgccgccg atcgctccaa gaccatcgcc gtggcgatca atcccgacag cacgcgcgac
      721 gacatcacca cgccgggcca tgtgttcccg ctgatggcgc gcgagggcgg caccctggta
      781 cgcgccggcc acaccgaggc gtctgtcgat atcgcccgga aagccggcct ggcgcctgcg
      841 gcggtgatct gcgagatcat gaacgacgac ggcaccatgg ctcggctgcc ggacctggtg
      901 accttcgcgc agcaccacaa tctgaaattg ggcacgatcg ccgatctgat cgcccatcgg
      961 cgcctgaccg aacggctggt gcgcaaggtc gaggagggtg cctatgccca cccggccggc
     1021 ggcgactggc gggccgtggt ctatgccaac acggtggaat accaggagca tctggcgctg
     1081 gttaagggcg acgtcagcgg caacgaacca gtgatggtac ggatgcatgc ggtcgatctg
     1141 ctggacgaca tgaccggatc cccgcactgg atcgcgcttc acaatgccat gcacatgatc
     1201 ggcaaggctg gccggggcgt cctggtgctg atccgggagc accgggccac cgcgcttgcc
     1261 gaacgcgtcc gcctgctggc cgccggcacg ccccggccgc agcgggaact gcgcgattat
     1321 ggcatcggcg cgcagatcct gctggatctc ggcataaaga acatgattct gctgaccaat
     1381 cgccggcgaa ccgtcatcgg tctcgagggt tacgggctga acatcatcga gcagcgcccg
     1441 atcccgaacg tcgtcggagt ggattacgaa ggatgagcac gcaggacgcc gaactgcccc
     1501 aagcgccgcg tgtcgaagga ccatcgcctc atttgttgat cgtgcgggca ccgtattatc
     1561 gcgacgtggt cgatgggctg accacaggcg ctaatcgcat cctggccgag gccggcgcaa
     1621 cggccgagac actggatgtg gccggtgctt acgaactgcc gcaggcgatc cgtatcgcgc
     1681 tgcgcggcag ccgcaaattc gatgggttcg tggcgctggg atgcatcgtg aagggcgaaa
     1741 cggatcatta tgacttcatc tgcaaagcga cgatggacgg aatcatgaac gtcgcactgc
     1801 aattcggtat cgcgctgggc accggcctgc tcacatgcga caccgtcgca caggctacgg
     1861 cccgatccaa ccaggatggc cacaacaaag gagccgaggc cgccgccgca gccctgctgc
     1921 agatcaacgt ggcccgccgc cttggtgcgg catgatggcg cgtccgccga aggttcgaac
     1981 acgcacggcg tcccgggtcg ctgccgttca ggctttgttc caggccgagc aggccggcga
     2041 taacccggaa acggtgatcg accagttcgt gcggcatcgg ctgggtgaac tggaaggtca
     2101 gtacggattc gaagatggcc ggattcccga tgccgaggtg aaactgttca gccgtgtggt
     2161 acgagaggcc gtgcggcgtc aggacgcgat cgacccgatg ctggtcgaag ccttgccggc
     2221 cgactggccg ctgtcacgga tcgaccccgt gctgcgggcg ctgatgcgtg ccgccgcggc
     2281 tgaattggcg atgccggacg gaccgccggc gaaggtagtc atcaacgagt acctggatat
     2341 tgcccgggga ttttttaccg gcccggagcc gggcctggcg aacgcggtgc tcgacagact
     2401 ggcccgtgtc ctgaagacgg cggaattcta ggtttccaca cgctttggac gttccgtcag
     2461 cgtgatcacg ctctagcgaa gcaaccagcg gaacgaatgc cgcagacgct gtgtagcgtc
     2521 ggtttcgaag cagtcgccat gagcaaagat cgcccgttcc ggctgcagcg ccagcatcct
     2581 tcgcaccgcc gtcaaagccg cctcgcgccg cattcggacg acgaagcgca aatacggcgg
     2641 tggcatgccg tccggcgcat tgctgccgaa catccgcacc accgagcgga ccaggacagg
     2701 caatttttcg gtttccaggt tcagcaccat atcggtcagc acgagtgtgc gggaagctc
//
LOCUS       DASWQN010000509         4681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2027533, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000509 DASWQN010000000
VERSION     DASWQN010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027533"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VGC82_08465"
     CDS             complement(<1..569)
                     /locus_tag="VGC82_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0423341.1"
                     /translation="MTALRDIAVVLDDSAASEIRLDIAVALAQQHGAHLTGLSALELL
                     IPTRTLVRPRGNPEVDTQQPASELLNIGAASPVDYPEADRQAAERAEQMEATFRERLR
                     FSQLLGEWRVVSGKVSESLVRQARQADLVILGQVNPDHPPPPEGRQLVEDVLMTSGRP
                     ILVIPYIGHFQTVGSRILVGWNNSREAARA"
     gene            1007..1282
                     /locus_tag="VGC82_08470"
     CDS             1007..1282
                     /locus_tag="VGC82_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423342.1"
                     /translation="MNCLELREMFASLSTQSQINVFVEFSLDADSSPGLLSRLLQPYA
                     KRDLIPDGMWSHRSGKIIHVEIAMEAMPAEMVHLVEGNLRQWWACGV"
     gene            complement(2277..3179)
                     /locus_tag="VGC82_08475"
     CDS             complement(2277..3179)
                     /locus_tag="VGC82_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY0423343.1"
                     /translation="MPGWKDILVRSWREVSENNIFLVAGGVTYAVLLALFPALAALVS
                     IYGLLLDPAQVERQVAALSNFLPQESTQMIGDQLHKLVTASSGSLSISAGIALLLALW
                     SASRGMIGLITAFNIAYEQKESRGFLKLNLVAVVLTVLMLVGGTVIIALVGVLPAVLQ
                     IIGLESATKWLLLVLEWPLLIAVVTTGLAVLYRYAPNREAPRWQWVSLGAIVASFLWL
                     LGSVAFTVYVTHFNSYDKTYGSLGGVVVMLTWLYLSAFVTLSGAVINAQSERQTQADT
                     TVGKDKPMGARRAHAADTLGEASS"
     gene            3334..3489
                     /locus_tag="VGC82_08480"
     CDS             3334..3489
                     /locus_tag="VGC82_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423344.1"
                     /translation="MNATLMAAASLAFLAAQDMVVDGDQVLIPPRLVDTFHIRLQVRI
                     HSAVSIG"
     gene            3534..3953
                     /locus_tag="VGC82_08485"
     CDS             3534..3953
                     /locus_tag="VGC82_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423345.1"
                     /translation="MSNLAWITRQQRQLAVPIPSRPGGDVALDQTADRNRCGGDTLIG
                     SPDGSKGAPLEQVELLAATGTGDHGSRATSRPDILRAANSVMVPQQPFFMGEAAQGSA
                     RAADGMARERGPAGSVKCLNLALSSMDRTTACSGGLT"
     gene            4326..>4681
                     /locus_tag="VGC82_08490"
     CDS             4326..>4681
                     /locus_tag="VGC82_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423346.1"
                     /translation="MKCGRGIVPICLALSACASNPLPPANAVRASFDSSAQAIEVVIS
                     NNQAPHDAFLVDADGRQYPVVLTLVSGPHVNYSAPPSIGLGLGGFGGGVGGGLGFGVP
                     LGGPRATGVDDQYVASA"
BASE COUNT          931 a         1415 c         1390 g          945 t
ORIGIN      
        1 gctcgcgccg cctcccggct attattccag ccgacgagga tcctgctgcc aaccgtctgg
       61 aagtggccga tataggggat aaccaggatc gggcgacctg aggtcatcaa aacgtcttcg
      121 accagttgcc gaccctccgg cggtggcgga tggtccgggt tgacctggcc aagaatgacg
      181 agatcagcct gtcgcgcctg gcgcaccagg gattcgctga ctttgccgct taccacccgc
      241 cactcaccca gcagctggct gaaccggagc cgctctcgaa aggtcgcctc catctgttca
      301 gccctttcgg ccgcctggcg gtctgcttcc ggatagtcaa cgggcgatgc ggcgccaatg
      361 ttcagcaatt cggaagcagg ctgctgcgta tccacctccg gattaccgcg aggccgcacg
      421 agcgtccgcg tcggtatcaa cagctccagc gcagaaagcc cagtgagatg ggcgccatgc
      481 tgttgggcaa gggcaacggc aatgtccagc cggatctcac ttgccgctga atcgtcgagc
      541 acgactgcaa tatcccgcaa agccgtcatc gtgtggcact ccttgctgcg aaccgcggcg
      601 gatcggtcac cgatgcaggt cgatcacgca ttttgacggc ctgttcttca agttcgtcaa
      661 gcatcaggtt gatcgaagtc agcgacatcc ggccagtgcg gcggcgtgat gcggacctgc
      721 ggacgatgtc cgccgtcgca ggtagcgcac gtgatcacca ctggatagtt attagcaatt
      781 cgataacatt gtcagataaa gaacgaaacc ggttgtggtg tcgcccacga ttatcaccgg
      841 gcaggcgtgt tgaaacaatc tggatctgcc tgctttgcct atgagcgcga tctcgtggct
      901 ccaggcagga ggtagggaag atgtccatct gccccttcgc ttgcgaggcg ataccaggaa
      961 tagcgagcta atcacccgca gccgggtcag tcggaccttc ttctcgatga actgcctgga
     1021 gcttcgtgag atgtttgcca gcctatctac gcaatctcaa atcaacgtct ttgtggaatt
     1081 ttcgctcgac gccgatagct cgcccggctt gttgtctcgg ctgctgcagc cgtatgccaa
     1141 gcgggacctg atccccgacg ggatgtggtc gcaccggtcc ggcaagataa tccatgttga
     1201 aatcgcgatg gaagcgatgc cggcggagat ggtgcacctc gtggaaggca atctacgcca
     1261 gtggtgggcg tgcggagtgt gacccgcatt tcccgggcgt gcctgctgca aagacttcgc
     1321 gaagttcatt ccttcgcatg gcaacctggg ccagcatgat ggaatcggcc gcatgagtct
     1381 cacctgtcgg gccgctaata cctcggccaa aaccctcctc gacgatccgg accaggggaa
     1441 ggcagatccc ttggattctg gcaaaggtcg caaacgcaag ccgtatagga ccctaagcgc
     1501 ccataggcgg ggatccagcc gcgacctttg catcttgagc aagattgctg agatgcctgc
     1561 ttttccgact tgatttgctg catgcgatca cggatcgcct tccagtcatt ggcgattggt
     1621 tccgccatgt acgtagtcct cccggatgcg gttatgcggc cgcggtattg cagcaacccg
     1681 ccatgcctga atccggttag cattgatccc ctctcgctgc tttgtcacga caccgcgccc
     1741 tggcgcagtg cccgcgtgcc ccccgctgat tgccctctcc ctctccctgg gccggcccgt
     1801 tatgccgcac cacatgcccc cgaacaccat tctcgccgcc gggatcatcg ccgcggcttt
     1861 ggctttgtcg tggtgatgat tcaagccggt gacgcggcgg ctggtgggat catctgatcc
     1921 aatggtgcga caggtggtcc gaccacagga accagcttca gcgctccttc cagcatgacc
     1981 atgatcaccg cgtccagggt gccgatgaac cgatcggcgc cgagtttccg gttccctttg
     2041 gtagaaatcg tattcggctc gtcgcgccgc gatgatcatg gctttcgttg gcaccattgt
     2101 tcaatatgaa tatgaaccga taggaattgc cgctataatg ccgtccggct cgatcacgct
     2161 ggtacaggtc gcggcccgca tcgcggccct cgccgttgcc tgctcccgct gcgacagggc
     2221 cggtcggtac aactggggcg cactgatcgc cggacatgga cccaggttcg gcattcctac
     2281 gaggacgctt caccgagagt gtccgcggca tgggctctgc gagctcccat cggcttgtcc
     2341 ttgcccacgg ttgtatcagc ttgggtctgc cgttcagatt gagcgttgat cactgcgccg
     2401 gacaaggtga caaatgcaga aagatacagc caggtcagca ttaccacgac cccgccaaga
     2461 gacccatagg tcttgtcgta gctgttgaag tgcgtgacgt agacggtgaa agcgaccgac
     2521 cccagaagcc atagaaagct cgcgacgatg gcgccgaggg atacccactg ccagcggggt
     2581 gcctcacggt tcggtgcata gcgataaagc accgccaggc cggtggtcac gacggcaatc
     2641 aacaacggcc actccagtac cagaagaagc cacttcgtcg cgctttccag gccaatgatt
     2701 tgaagcactg ccggtagcac cccaaccaag gcgatgatca ccgtcccgcc gaccaacatc
     2761 aacaccgtca atacgactgc caccaaattg agcttgagga acccgcggct ttccttctgc
     2821 tcgtaggcga tgttgaacgc cgtgatcagg ccgatcatgc cgcgcgaagc gctccataat
     2881 gccagcagca gggcgatgcc ggcgctgatg ctcaacgatc cgctagatgc cgtgacaagc
     2941 ttgtgcagtt gatcgccgat catctgcgta ctttcctgcg gcagaaagtt cgacaacgcg
     3001 gcgacttgcc gttccacctg ggccgggtcc agcaacaagc cgtagatcga aaccagggca
     3061 gcaagggccg gaaacaaggc cagcagcacc gcgtaggtca ccccgcctgc gacgaggaaa
     3121 atgttgttct ccgacacctc tcgccacgat cgaacaagaa tgtccttcca gcccggcatt
     3181 gggatatgct gcggggaggt ggcagagcgc cccctgttct gttgcgggga tggcttctgg
     3241 tctacgcgct ggggcacaat cgcatccaat tctcgcttca atttgtccag ccggggtgat
     3301 gaagtcatta aacgcttcct ttgcggcagg cggatgaacg caacgctaat ggcggcggcc
     3361 agcttagcgt tccttgcggc gcaggacatg gtggtggatg gcgatcaggt acttatcccg
     3421 ccccgcttgg tggacacgtt ccacatccgc ctgcaagtgc ggatccattc cgcggtatcg
     3481 atcggctaag gacgctggat cagggcccgc ggatcctcga accacttcgt ccgttgagca
     3541 atctcgcctg gatcacccgc cagcagcgcc agttggcggt cccgattcca tcgcgtcctg
     3601 gcggggatgt tgcgctcgac cagactgctg atcgaaaccg ctgtggaggc gataccctga
     3661 tcggcagccc ggatggatca aaaggcgcgc ctctggaaca ggtcgaactg ttggcagcaa
     3721 caggaacggg agatcacggt agccgcgcaa cgagccgccc tgacatattg agggcggcga
     3781 acagcgtcat ggtgccgcag cagccctttt tcatgggcga agctgcccaa ggttccgccc
     3841 gcgcagcgga tggaatggca agggagagag gcccggctgg ttcggtcaag tgcctgaatt
     3901 tggctctttc gtcgatggac aggacgacgg catgctccgg cggattgacg tagggccgac
     3961 gatatcaagc agtttagcgg caaactgcgg accgttggac agcttgaact gatgcaactc
     4021 gtgcggctgg aggccgtgct tgcggcagga atccacaggc atgggcaggt ggcctgcctc
     4081 caaccaactg cggaaatttt ccagtccatg gcaaggacgg caaagttctg cccgaatgtc
     4141 ccgagcgagc ataagttaaa gcagacgtgt gaaaaataca tataatgaag gaaatggaat
     4201 ttatttactt cactgagcgc ctcgaacgct gggggcaagc ttcatatcac ggctctttgc
     4261 gggcatgccg cttgatacct cgttgcgggc tacacaggta tttgtcgtaa gctgttgaga
     4321 aaatcatgaa gtgcggtcgt ggcatcgtgc ctatctgcct cgcgctcagc gcctgcgcga
     4381 gtaaccctct gccgccagcg aacgccgtgc gcgcctcatt cgattccagc gcccaagcca
     4441 tcgaagtcgt tatcagcaac aaccaagcgc ctcacgatgc cttcctcgtg gatgcggacg
     4501 ggaggcaata tccggtcgtg ctgacgctgg taagtggccc gcacgtcaac tacagcgcac
     4561 ctccaagtat cggcctcggt ctcggcggct tcggtggtgg tgtcggtggc ggcctcggtt
     4621 tcggcgtgcc gctcggtggg cctcgtgcga ccggcgttga cgatcagtat gtcgcgtcag
     4681 c
//
LOCUS       DASWQN010000510         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1631834, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000510 DASWQN010000000
VERSION     DASWQN010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631834"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VGC82_08495"
     CDS             complement(<1..261)
                     /locus_tag="VGC82_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0423347.1"
                     /translation="MPLLPLHTDAKIVRVQLRQNLALRHLSATQWQELEPLLDVEDYP
                     KGHTLEHQGDHSMEQYFILDGILKRVVSNAGGKEMILRFAAET"
     gene            545..>1555
                     /gene="oxc"
                     /locus_tag="VGC82_08500"
     CDS             545..>1555
                     /gene="oxc"
                     /locus_tag="VGC82_08500"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEY0423348.1"
                     /translation="MDMTTDTKLEVTDGFHLVIDALKLNGIDTIFGLPGIPITDLTRM
                     AQAAGMRVISFRHEQNAGNAAAIAGFMTQKPGVCLTVSAPGFLNGLTALSNATTNCFP
                     MILISGSSEREVVDLQQGDYEEMDQLAVAKPLCKAAFRVLHAEDIGVGIARAIRAAVS
                     GRPGGVYLDLPAKLFAQTLDAEAGKKSLIKVVDPAPRQIPAPDAVERALELLKSAKRP
                     LIVLGKGAAYAQADADIRALVEKTGIPYLPMSMAKGLLPDTHEQSAAAARSYVLPEAD
                     VVMLIGARLNWLLSHGKGKTWGGKDPKAWGRQKFIQIDISPQEADSNVRIDAPVVGDI
                     GSC"
BASE COUNT          296 a          499 c          476 g          284 t
ORIGIN      
        1 agtttcggcc gcgaagcgca ggatcatctc cttgcccccg gcgttggaca cgacgcgttt
       61 caatatgccg tcgaggatga agtactgctc catcgagtgg tcgccctggt gttcgagcgt
      121 gtggcctttg ggatagtcct cgacgtcgag cagcggctcg agctcctgcc actgcgtcgc
      181 gctgagatgc cgcagcgcaa gattctgccg cagttgcacg cggacgatct tggcatcggt
      241 atggagtggg agcaggggca tgttggcacc gccgcaaggg ccacccgcga ccgacgcagt
      301 atagcaattc gctgtcggta cccctgcatg caccggcgaa agggccgacc ccggcgcgag
      361 tgtcaccact atacgaatct tgaccgcggt caagggctca tatgcatgcg ggcgtctatc
      421 ataggtcgca gtaaacacgc cacatttcgg ctggctcgat ctgccgtgaa ccaaggtagg
      481 ccacggcaga tacacaccgg aacgaccctg ttcacacgac ggcgagaacg cgaggcatct
      541 caacatggac atgacaactg atacgaaatt ggaagtgacc gacggctttc atctggtcat
      601 cgatgccctc aagctcaatg gcatcgatac tatctttggt ctgcccggca tcccgatcac
      661 cgacctcacc cgcatggcgc aagccgcagg tatgcgcgta atttcattcc gtcatgagca
      721 gaacgccggt aatgcggcgg ccatcgcggg cttcatgacc caaaagcccg gcgtctgttt
      781 gacggtttcc gccccgggct tcctcaacgg ccttacggcg ctgtccaacg ccaccacgaa
      841 ttgcttcccg atgatcctca tcagcggctc cagcgagcgc gaggtcgttg acctgcagca
      901 gggtgactat gaggagatgg atcagttggc cgtcgccaag ccgctatgca aggccgcctt
      961 ccgtgtgctg catgccgagg atatcggtgt gggcatcgcg cgcgccatcc gcgccgcggt
     1021 ctccggccgc ccaggcggcg tctacctcga cctgccggca aagctctttg cccagaccct
     1081 ggacgcagaa gccggcaaga aatcgctaat caaggtggtt gatccggcac cgcgccagat
     1141 ccccgccccg gatgccgtgg agcgcgccct cgagctcctt aagagcgcta agcgcccgct
     1201 gatcgttctg gggaagggcg ccgcgtacgc gcaggccgat gcggacatcc gcgcgctcgt
     1261 cgagaagacc ggcattccct acctgccgat gtcgatggct aagggcctgc tgccggatac
     1321 ccatgagcag tccgcagccg cggcccgttc ctatgtgctg cccgaggcgg acgtggtcat
     1381 gttgatcggc gcgcgtttga actggctgct ctcgcacggc aaaggaaaga cctggggcgg
     1441 caaggacccc aaggcctggg gccgccaaaa gttcatccag atcgacatct cgccccagga
     1501 ggcggatagc aacgttcgta tcgacgcgcc ggtggtcggc gacatcggtt cctgc
//
LOCUS       DASWQN010000511         1886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2291595, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000511 DASWQN010000000
VERSION     DASWQN010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2291595"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VGC82_08505"
     CDS             complement(<1..483)
                     /locus_tag="VGC82_08505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0423349.1"
                     /translation="MADRILVFYGSYRFDRMGIRLAHYVVARLLARGADAELIDAKAI
                     GLPILERMYKEYPAGTAPAGMQQLAQKIGAADGFVFIAGEYNWGMQPGLKNLTDHFLE
                     EWFWRPAAIASYSAGRLAGARAAVAWHGTLSEMGMAVTSSTLAVGSISQALSEDAAPV
                     G"
     gene            681..1535
                     /locus_tag="VGC82_08510"
     CDS             681..1535
                     /locus_tag="VGC82_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0423350.1"
                     /translation="MNYPQPPFPSQQQPMPGETNAMKPVPDHGETSYKGSGRLQGKKA
                     VITGGDSGIGRAVAIAFAREGADVLISYLSEDRDARETERLVKEAGTKAVLVPGDLQN
                     PDHCRAVIAKAVAEFGGIDILVNNAAHQATFKSIEDISDDEWELTFRVNIHAMFYLTK
                     AAVPHMKPGSCIINTASINADAPNPTLLAYATTKGAIQNFTAGLAQMLAEKGIRANAV
                     APGPIWTPLIPSTMPEEAVKNFGKQVPMKRAGQPAELATAYVMLADPLSSYVSGATIA
                     VTGGKPIL"
     gene            1635..1835
                     /locus_tag="VGC82_08515"
     CDS             1635..1835
                     /locus_tag="VGC82_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423351.1"
                     /translation="MEQLGKPVPDVCIIGHIGKQAFLHWLWEIWPKLESRPAKDFLKV
                     RHRQSPSLNGPIKNTRRWLLFL"
BASE COUNT          379 a          614 c          576 g          317 t
ORIGIN      
        1 cccgacgggc gcggcgtctt cggacagcgc ctgcgaaatg ctgccgaccg ccagcgtgct
       61 cgatgtgacg gccatgccca tctcggaaag cgttccatgc caggcgaccg ccgcccgcgc
      121 tcccgccagc cgtcccgccg aatagctggc gatggcggcg ggacgccaga accattcctc
      181 gagaaagtgg tcggtgagat tcttcagtcc tggctgcatt ccccaattat actcgcccgc
      241 gataaagaca aagccgtcgg cggcgccgat cttctgcgcg agctgctgca tccccgccgg
      301 ggccgtgccg gccggatatt ccttgtacat ccgctccagt atcggcagac cgatcgcctt
      361 ggcgtcgatc agttccgcat cggcgccgcg cgcgagcagc ctggcgacca cataatgggc
      421 gagccggatt cccatccggt cgaaccgata ggagccgtag aaaacgagga tgcgatcagc
      481 catggacctt gcctcggata agcctgggcg cgcgacaggt caccctatcc ggcattcgag
      541 gttgtaggcg ttgcaatacc gaggtgcaat cgcaggcgta cggaagcttc cgcgacgctg
      601 gaatccacgc gtcgaggccg gaacgaacaa tccgcgacag ggttttctgc attccaggcc
      661 cccccgacgc ggagcgaaaa atgaattatc cccaaccgcc atttcccagc cagcaacagc
      721 cgatgcccgg cgagaccaac gccatgaagc cggtgcccga ccatggtgaa acttcctaca
      781 aggggtcggg tcgcctgcag ggcaagaagg ccgtcatcac gggaggcgac agcggcatcg
      841 gccgcgcggt cgcgatcgcg tttgccaggg agggcgccga tgtcctgatc tcctatcttt
      901 ccgaagaccg cgacgcgcgc gagaccgaac gcttggtcaa ggaagcgggc accaaggccg
      961 tgcttgttcc cggcgatctg caaaatccgg atcactgccg cgcggtcatc gccaaggcgg
     1021 tcgcggaatt cggaggcatc gatatcctgg tcaacaacgc cgcgcatcag gcgaccttca
     1081 agtcgatcga ggacatttcc gacgatgagt gggaattgac gttccgggtg aacatccatg
     1141 cgatgttcta ccttaccaaa gccgcggtcc cgcacatgaa gccgggaagc tgcatcatca
     1201 atacggcgtc gatcaatgcg gacgcgccga acccgacgtt gctggcctat gcgaccacca
     1261 agggggccat ccagaacttc accgcggggc tggcacagat gctggccgag aaaggaatcc
     1321 gcgccaatgc ggtcgcaccc ggcccgatct ggacgccttt gattccgtcg accatgcccg
     1381 aggaggccgt caagaatttc ggcaagcagg ttccgatgaa acgcgcgggg cagcccgccg
     1441 aactcgccac cgcgtatgtc atgctggccg acccgctctc cagctatgta tccggcgcga
     1501 cgatcgcggt gaccggtgga aagcccatcc tgtaaagcct ccgcccagcc gcgcccgggc
     1561 agcctgcacg gtccttccgc tggctatgcg agcgcggtcg gctttggagg gtgcgcggtc
     1621 ctgggatggc ggtcatggag cagttgggca agcccgttcc tgatgtctgc atcatcgggc
     1681 acataggcaa acaggcgttc ctgcactggc tgtgggagat ctggccaaag ctcgagagcc
     1741 gccctgccaa ggattttctc aaagtccggc atcgccagtc cccttcgttg aatggaccga
     1801 tcaagaacac ccgaagatgg ctgctgttcc tgtgaccacc gtcgcgaaag tcagcaacct
     1861 caatagagag caggcggctt cccaca
//
LOCUS       DASWQN010000512         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2423364, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000512 DASWQN010000000
VERSION     DASWQN010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2423364"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..521
                     /locus_tag="VGC82_08520"
     CDS             60..521
                     /locus_tag="VGC82_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY0423352.1"
                     /translation="MFRIGIISDTHGQLRPEAERRLAGVDHIVHAGDIGRPEIIDTLR
                     RIAPVTAIRGNVDSAGWAREYPDTALLRLAGISIYVLHDLKTLQADPGAGIDVIVSGH
                     SHVPKIKTVGGVLYLNPGSAGRRRFNLPITLATLAVTPDGMRPEIHDLGGA"
     gene            complement(543..1115)
                     /locus_tag="VGC82_08525"
     CDS             complement(543..1115)
                     /locus_tag="VGC82_08525"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY0423353.1"
                     /translation="MNAADHLTERPVQRASVVAVALDAKLRFSKTPRLSVILTAGYGV
                     EGDAHYGQFVRHRHLARRKPRAPNLRQVHLIPSELLNALRKSGFDVSPGDLGENITTA
                     GLDLETLPLNTELYIGASANIRLTGLRTPCILIDRFKSGLKSRLLTGTPNLPFRAGVM
                     AVVSEGGEISPGDPIRAILPERPHLPLPPL"
     gene            complement(1112..1324)
                     /locus_tag="VGC82_08530"
     CDS             complement(1112..1324)
                     /locus_tag="VGC82_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423354.1"
                     /translation="MEEAILQPSKDEEVMNRTAEVAPADAEQYLQAGGECVPDRLSRE
                     RRSQLRDGRHFAVDRDVLASNGQPPS"
BASE COUNT          318 a          525 c          570 g          343 t
ORIGIN      
        1 ggacaggtag agcttctcga tcgtctcttg gcgatcgcgg gtgagagcgc ggggtaaggg
       61 tgttcaggat tggaatcatt tcggatacgc atggccagtt gaggcccgag gcggaacgac
      121 gcctggcagg tgtcgatcac atcgtacatg ccggcgacat cgggcgcccc gagatcatcg
      181 acacgcttcg ccggattgcg cccgtcaccg ccatccgcgg gaacgtggac agtgcagggt
      241 gggcccgtga atatcctgac acggcactcc ttcgcctggc gggaatctcg atctacgttc
      301 tgcacgatct gaagacgctt caggccgatc ccggcgcggg catcgacgtg atcgtctccg
      361 ggcattccca cgtaccgaag atcaagacgg tcggcggcgt cctctacctc aatcccggca
      421 gcgcgggacg gcgacgattc aacctgccga ttacgcttgc gacgcttgcc gtcacgcccg
      481 atggcatgcg accagaaatc cacgatcttg gaggcgcctg aacgcgccgc ggcggaggga
      541 attcatagcg ggggaagagg caggtggggg cgctcgggaa ggatggcgcg gattgggtcg
      601 cctggtgata tttcgccgcc ttcggacacg accgccatga ccccggccct gaagggcaga
      661 ttcggtgttc cggtcagtaa tcggcttttc aatccggact tgaagcgatc gatgagaatg
      721 catggcgtgc ggaggccagt gagccgaatg ttcgccgagg cgccgatata cagctccgtg
      781 ttcaaaggca gcgtctccag atcgagcccc gccgtcgtaa tattttcgcc gagatcgccg
      841 ggactaacgt cgaagccaga ctttcgcaaa gcattgagca gttcgctcgg gatgaggtgg
      901 acctgccgca ggtttggcgc ccgcggcttt cggcgcgcca gatgtcggtg ccggacaaat
      961 tggccgtagt gagcgtcgcc ttcgaccccg tatcccgctg tgagaatgac ggagaggcgg
     1021 ggagtcttgc tgaaccgaag cttggcatcg agcgctacgg cgacaaccga tgcccgctga
     1081 acaggccttt ctgtgaggtg gtccgccgcg ttcaacttgg cggctgtccg ttcgaggcca
     1141 agacgtcacg gtccaccgcg aagtggcgcc cgtcacgaag ctggcttcgt cgctcgcgag
     1201 aaagacgatc agggacgcac tctcctccgg cttgcagata ttgctcggcg tcggcgggtg
     1261 cgacttcggc ggtgcggttc atgacctctt catctttgct cggttggaga attgcctctt
     1321 ccatgcaacg aggtaccggg ccaaaacgct gcccgccctc gtacataaag gcgaatgcgt
     1381 aaattcgttc cgtttgccgg tcaggctcga acggatcgct atttacgtga acgttctcgc
     1441 tcgcgcacga catccttcgc ccgcttgtcg gacctgagcc cgaggtagac gggctggcgc
     1501 agttcgccct tgctagtcca ctccgcgaat ttgacctcgg caaccagcga gggctggacc
     1561 caggtcgtgt tgcctcgtcc ttcactttgg caggaaaggg tgacatcgag gccgtcagct
     1621 tcacgagctt ggcatggagg tcttccagga ccttgtggct gaagccggtg ccgacatgtc
     1681 cgatgtagcg ccatgcgtcg ccttcccgca cggcgaggac aagcgcgccg aagaacggcc
     1741 tggtgcgcct gggcgc
//
LOCUS       DASWQN010000513         5078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_576412, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000513 DASWQN010000000
VERSION     DASWQN010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576412"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VGC82_08535"
     CDS             <1..941
                     /locus_tag="VGC82_08535"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0423355.1"
                     /translation="MSPYRPHLTMRAWRRIIGFSFWTWAQTMLYQARERSDSIVIGRL
                     LGTGQVGVFSVGVELGSLPTTELVEPLGRALFSGFASLHNASEGLGGMFLSAVGLGFL
                     LILPAGVGISMVADPMVRLSLGEHWLSAVPVVQIVAISGTVAIFTQSCANLLNAVGRP
                     RITAYIGLASTLVKLVALVLLVPYFGLPGAAMALLLSSVTDLVLLLRVTLPRIGVSAG
                     RLAACVVRPAAATAAMVAVLWQLGLAWTPSSGADVMAISGDAGTRSAIGAACYGLVLA
                     GMWFAAGWPDGAERSAVAIADRTWRRVFQRAGLGQP"
     gene            980..1834
                     /locus_tag="VGC82_08540"
     CDS             980..1834
                     /locus_tag="VGC82_08540"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY0423356.1"
                     /translation="MPDTTTIHTAIWRPSRLRSILASCAYPVVLALNRPSMSWFGDLL
                     YDIALRCNGIAITFAGKAGLTRAEEHFLHRNKDRFQDGILFDVGANHGAYALILNKLA
                     PTARIFAFEPHPTTFEFLRLRTAGSPPIRAVNKAVADKAGRLKLYDFRSEDGSTQASL
                     SESAVALYSADIVEHSVDSTSIDQYMAEEGLDAIDLLKIDTEGHDLSVLEGARNALLR
                     RKIRMIQFEFIPANIATGATMHGFFEALRGYRIGRLCLNGTVRWLDRYDVKRCEIYVT
                     HNLIAVPR"
     gene            complement(1851..2582)
                     /locus_tag="VGC82_08545"
     CDS             complement(1851..2582)
                     /locus_tag="VGC82_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY0423357.1"
                     /translation="MGGIAIGGKAIYGAPLGILMLEARFPRIPGDMGNATTWPFPVLY
                     RVVKGATPERVVLQGAAGLLPDFLDAAAELVSFGAEAITTNCGFLSLFQQEIAAHVGV
                     PVATSSLIQVPWVQATLPPGKLVGVITVSASSLTPRHLEAAGAAVDTPFAGTETGKEF
                     FRVLILGQKQDMDIALAAEDVLDAGRRLVAEHPEIGAIVLECTNMPPYAHALREVLGM
                     PVYDIYSLITWLHAGIRPRNFGVLA"
     gene            2724..3419
                     /locus_tag="VGC82_08550"
     CDS             2724..3419
                     /locus_tag="VGC82_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEY0423358.1"
                     /translation="MAERPFTQADLDVLAQWDTPTICNGLEIVVPERRAFGFTVEQMV
                     CIDPKAKPIVGLARTGMIRATEPPRSKVADRADYYDYVARVDFPTISVLQDIDGRVGY
                     GAYWGEVQSTIHKALGAIGCVTNGSFRDLDMWAPGFQMIGGRVGPSHAHVHMVDFGKP
                     VNIFGMHVAHDDVIHADFHGAVVVPADAVRKLPAAIDLVSRREAVILEVCKAPGFTPA
                     KLREALRKSGEIH"
     gene            complement(3460..4233)
                     /locus_tag="VGC82_08555"
     CDS             complement(3460..4233)
                     /locus_tag="VGC82_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY0423359.1"
                     /translation="MTVRIRRDQGGAVAIVSIDNQARLNCLGRDQITAFIDIVSALHD
                     DPELRVLIVTGEGDRAFMGGANLHELGQLNPPKARDFLVLIHRMCKVLRDLPVPVIAR
                     VNGFCLGAGLEVMAACDMHIASDNAVFGMPEVKIGLPSVVEAALLPQLIGWGRTRLLL
                     YTGDNIDARTALDWGLVEKVVPAAELDAALGKWVHSIVESGPKAIRLQKELIREWEAM
                     PVNDAIEAGIRSISRAYQSEEPGRMIDAAIQRLKARKRG"
     gene            complement(4230..>5078)
                     /locus_tag="VGC82_08560"
     CDS             complement(4230..>5078)
                     /locus_tag="VGC82_08560"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0423360.1"
                     /translation="RKHGEVAKPLTRRGRMQMIALQDRFAGKVAVVTGAASGIGLAIT
                     KRLLAEGAKVVGTDLNAVDGLGDGFVGIRGDVTSEAAAEELVATAVERFGAVHAAFNV
                     AGGARAGYIVDLAESDWDFTVDLCLKGVFLGMKHQARQMMRQGAGSIVNVASLNAHVP
                     MHAGSAYVAAKAGVEMLSRNGALELAEFGIRVNAILPGLVQTNLTRRHFDNPDALAAF
                     EQRIPMGRPAQPEEIAAPALYLASDDATYVNGASLLVDGAWAVSGYPDMRPFRGPIGW
                     NQRTKA"
BASE COUNT          858 a         1615 c         1649 g          956 t
ORIGIN      
        1 tgatgtcgcc gtatcgcccg catctcacca tgcgggcgtg gcgtaggatc atcgggtttt
       61 cattttggac ctgggcccag acgatgcttt atcaggcacg ggaacgttcc gacagcatcg
      121 tgatcggccg cctgttgggc accggacagg tcggcgtctt ttcggttggc gtggaacttg
      181 gctcgctccc gacgaccgag ttggtggagc ccttgggccg cgccctgttc tctgggttcg
      241 catcgttgca taacgcgtcc gaggggctgg gcggcatgtt cctgagtgcg gtcgggttgg
      301 ggtttctgct gatcctgccg gcgggggttg gcatatcgat ggtcgccgac ccgatggtgc
      361 ggctgtcgct gggcgagcat tggctgagtg ccgtgcccgt ggtgcagatc gtggcgatca
      421 gtggaacagt cgcgatcttt acccaatcct gcgccaacct gctgaacgcc gtcggccggc
      481 cgcgcatcac cgcgtatatc ggtcttgctt cgacattggt gaagctggtc gcgctggtcc
      541 tgctggtgcc gtatttcgga ctgccaggag ccgccatggc acttttgctg tcaagcgtca
      601 ccgatctggt gctgctgctt cgggttacgc tgccgcggat cggcgtgtcg gccgggcgcc
      661 tcgcggcttg cgtcgtcagg ccggccgccg ccacggcggc gatggtggcc gtgctttggc
      721 agttggggtt ggcatggacc ccaagcagcg gggcggatgt catggctatc agcggagacg
      781 ccggaacaag gtctgcgatc ggcgcggcct gttatggcct tgtcctcgcc gggatgtggt
      841 tcgcggccgg ctggccggac ggagcggagc gatcggcggt ggcgatcgca gacaggacat
      901 ggcggcgggt ttttcaacgt gccggtcttg gacagccatg atcgttccgc gcagcacgta
      961 tcgccaaagg tgacgacgga tgcctgacac gacgacaatc cacaccgcga tctggcggcc
     1021 ctcgcggctt agaagcatcc tggcctcctg cgcctatccg gttgttctcg cattgaaccg
     1081 gccgtccatg agctggttcg gtgacttgtt gtacgacatc gcactgcgtt gtaacggcat
     1141 cgccattact ttcgccggca aggcaggcct gacccgtgcc gaagagcatt tcctgcatcg
     1201 aaacaaggat cggttccagg atggcatcct gttcgatgtc ggtgccaacc atggcgccta
     1261 tgccctcatt ctcaacaagc tggccccaac ggcgcgcatt ttcgcgttcg aaccgcatcc
     1321 gacgacgttc gagttcctgc gcttgcgcac agcgggttca ccgccgattc gcgcggtcaa
     1381 caaagccgtc gcggacaagg ccggccggtt gaagctttac gacttccgat ccgaggacgg
     1441 gtcgacgcag gccagcctca gcgaatccgc cgtcgcacta tactcagcgg acatcgtgga
     1501 acattcggtt gatagcacca gcatcgacca gtacatggca gaagaaggtc tcgatgccat
     1561 tgacctgttg aagatcgaca ccgaggggca cgatctttcg gtcctggaag gtgctcggaa
     1621 tgcattgctg cgaagaaaaa tccggatgat ccagtttgag ttcattccgg ccaatatcgc
     1681 gaccggcgcg acgatgcatg ggtttttcga agcgttgcgc ggataccgca tcgggcggct
     1741 ctgcctgaat ggcacggtac gatggctcga tcgctatgat gtaaagcgct gcgagatcta
     1801 tgtcacgcac aacctgatcg ccgtaccgcg atagctcgat cgttgcccgg tcaggcaagc
     1861 acgccaaaat tgcgtggtct tattcctgcg tgcagccagg taatgagcga gtaaatgtca
     1921 tagaccggca tcccgagtac ctccctcagc gcgtgcgcat aaggcggcat gttggtgcat
     1981 tccagcacga tagcgccgat ctccggatgt tcggcaacca gccggcggcc ggcatccaga
     2041 acgtcctctg ccgccagggc gatatccata tcctgcttct ggcccaggat caggacccgg
     2101 aagaactcct tcccggtctc ggtcccggca aaaggcgtgt ccaccgccgc gcccgctgcc
     2161 tcgagatggc gcggggtcag ggacgaggcg gacacggtga tcacgccgac cagctttccc
     2221 ggcggcagcg tcgcctgcac ccagggtacc tggatcaacg aacttgttgc caccggaacg
     2281 cccacatgag cggcgatctc ctgctggaac agcgacagga agccgcagtt ggtggtgatc
     2341 gcctcggccc cgaacgacac cagttctgcc gcggcgtcca ggaaatccgg cagcagtcca
     2401 gccgcccctt gcagcaccac gcgttccgga gtggcgccct tcaccacgcg gtacagcacc
     2461 ggaaacggcc aggtcgtggc gttgcccatg tcgccgggaa tgcggggaaa gcgcgcctcc
     2521 agcatcagga tgccgagcgg cgcgccgtaa atggctttgc ccccaatggc gatgcccccc
     2581 aatggcgatg cctgcggtgg caatgccccc ggttgcggtg cccccggttg cggtacttgt
     2641 gatcgatgtc atgccgccat cttgccgcga acgtgctcgc cgggcgagta tgcctgtcgc
     2701 ttcgacaaag agaggaaacc atcatggccg aacgtccctt cacccaagcc gacctggatg
     2761 tgctggctca gtgggatacg cccaccatct gcaacgggct ggagatcgtg gtgccggagc
     2821 ggcgcgcctt cggcttcacg gtcgaacaga tggtctgcat cgacccaaag gccaagccaa
     2881 tcgtcggctt ggcccggact ggaatgatcc gcgcgaccga accgccacgc tccaaggtcg
     2941 ctgaccgagc agattattac gattatgtcg cgagggtcga cttccccacc atctccgtat
     3001 tgcaggacat cgacggccgg gttggctacg gtgcttactg gggcgaggtc cagtccacga
     3061 tccacaaggc gctgggcgcc atcggctgcg tcaccaacgg gtcgttccgc gacctggata
     3121 tgtgggcgcc agggttccag atgatcggcg ggcgggttgg gccgagccac gcgcacgtcc
     3181 atatggtgga tttcggcaag ccggtgaaca ttttcggcat gcatgtggcg catgacgacg
     3241 tgatccacgc cgacttccac ggtgcggtcg tcgttccggc cgatgccgtg cgcaagttgc
     3301 cggcggcgat cgatctggtg tcgcgccgtg aagcggtgat cctggaggtt tgcaaagccc
     3361 ccggctttac gccggccaag cttcgcgagg cactgcggaa gtccggcgag atccactgac
     3421 gtaagcagcc ggccggcctt catgcggccg gccggcatat cacccgcgtt tgcgggcctt
     3481 cagccgctgg atcgccgcgt cgatcatccg cccgggttcc tcgctctggt aggctcggga
     3541 tatgctgcgg atgccggctt cgatcgcgtc gttgaccggc atcgcttccc actcgcggat
     3601 cagttccttc tgcaggcgga tcgccttcgg ccctgattcg acgatcgaat gtacccattt
     3661 gcccaaggcc gcatccagtt cggccgcggg aacgaccttc tccaccaagc cccagtccaa
     3721 cgcggtgcgc gcgtcgatat tgtcgccggt gtacagcagc aaccgggtgc ggccccagcc
     3781 gatcagttgc ggcagcagcg cggcctccac gaccgacggc aggccgatct ttacctccgg
     3841 catgccgaac acggcgttgt cgctggcgat gtgcatgtcg caggcggcca tgacctccag
     3901 ccccgcaccc aggcagaagc cgttgacccg ggcgatgacg ggaacgggca ggtcgcggag
     3961 gaccttgcac atgcggtgga tcagcaccag gaagtcgcgc gccttcggtg ggttgagctg
     4021 accgagctcg tgcaggttgg cgccgcccat gaaggcgcga tcgccttcgc cggtgacgat
     4081 caggacacgc agctcggggt cgtcgtgcag ggcggagacg atgtcgatga atgcggtgat
     4141 ctggtcgcgc ccgaggcagt tcaggcgggc ctggttgtcg atcgacacga tggcgacggc
     4201 gccaccctgg tcgcggcgga tgcggaccgt catgccttgg tcctctggtt ccagccgatg
     4261 gggccgcgaa acggtctcat gtccgggtag ccgctgaccg cccaggcgcc gtcaaccagc
     4321 aggctggcgc cgttgacgta ggtcgcatcg tcgctcgcca gataaagtgc cggggcggcg
     4381 atttcctcgg gctgagccgg gcgccccatc ggaatgcgct gctcgaaggc cgcaagcgcg
     4441 tcgggattgt cgaaatggcg gcgggtcagg ttggtctgca ccaacccggg caggatggcg
     4501 ttgacccgta tgccaaattc cgccaactcc agggcgccgt tgcgcgacag catctccacg
     4561 cccgccttgg cggcgacgta cgccgaaccg gcgtgcatcg ggacatgcgc gttcaacgag
     4621 gcgacattga cgatgctgcc ggcgccctgg cgcatcatct gccgcgcctg gtgcttcatg
     4681 ccgaggaaga cgcctttcaa gcaaagatcg accgtgaagt cccagtcgga ctcggcgaga
     4741 tcgacgatgt agccggcgcg ggcaccgccg gcgacgttga acgcggcgtg cacggcaccg
     4801 aaacgctcca cggcggtggc aaccagttct tccgccgcgg cctcgctggt gacgtcgccc
     4861 cgtatgccga cgaagccatc gccgaggcca tcaacagcgt tcaggtcggt gccgacgacc
     4921 ttggcgccct cagccagcag gcgcttcgtg atggccaatc cgatacccga ggcggcaccc
     4981 gttaccaccg cgaccttgcc ggcgaaccgg tcctgcaacg caatcatctg catcctcccg
     5041 cggcgcgtta agggtttggc aacttctccg tgtttccg
//
LOCUS       DASWQN010000514         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_971947, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000514 DASWQN010000000
VERSION     DASWQN010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971947"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="VGC82_08565"
     CDS             complement(<1..147)
                     /locus_tag="VGC82_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEY0423361.1"
                     /translation="MALPSSFTEELRTRTPLASIVGRRVRLVRSGKQWKGCCPFHGEK
                     SPSFY"
     gene            complement(226..684)
                     /locus_tag="VGC82_08570"
     CDS             complement(226..684)
                     /locus_tag="VGC82_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY0423362.1"
                     /translation="MGLRETFTEQVKLSMKAGNAARTSTLRMVMARLKDIDIASRPKG
                     IDKVPDEEIIAMLRGMVKSRRESVELYNKGNRPELAAKEEAEIAVIESFLPSQMDAAA
                     MEQAVADAVKQTGAASIKDMGKVMGVLKAKHAASMDMALAGPMVKARLGG"
     gene            complement(736..1233)
                     /locus_tag="VGC82_08575"
     CDS             complement(736..1233)
                     /locus_tag="VGC82_08575"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY0423363.1"
                     /translation="MYKTKPAMTGGCQCGALRYALYETPESSVCHCRMCQKAVGGPFA
                     AFSKVPLDRFVWTRGETASYRSSSVAERRFCAACGTPLTFRYLDGEAIEVTTASLDTP
                     ELAPPTKNYGVEARLGWIHLVAPGVLPDTPATESGSATRVVISRQHPDEETPDTWRPP
                     TPTSA"
     gene            1316..>1972
                     /locus_tag="VGC82_08580"
     CDS             1316..>1972
                     /locus_tag="VGC82_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HEY0423364.1"
                     /translation="MPISTDHIIERLGGADAAARLTGVGTEAVRKWRQSRSIPSRHWP
                     AVVAATGLTMADLQSSDQASAPMSDATTVPPAGATAVLVLADGTEFWGRGFGAHTSRT
                     GLGVHTPRPREATHPDDRARAAGKVGEVCFSTGMTGYQETLTDPSFAGQIITFTFPHI
                     GNVGTNRDDIEATTIAARGLVVKQDITEPSNWRSTQTLQSWLRAQGVTGIAGVDTRTL
                     T"
BASE COUNT          332 a          650 c          604 g          386 t
ORIGIN      
        1 atagaagctg ggcgactttt cgccgtggaa cgggcagcag cccttccatt gcttgcccga
       61 ccggaccaac cgcacccgcc gcccgacgat cgatgccagg ggggtgcgag tgcggagttc
      121 ctcggtgaag ctggacggca gcgccatctg aagactattc cgccatcagc ttgtttgatc
      181 gcatgattca cacgctttga acgtttcgct cagcgtgatc atgctctagc cgcccaggcg
      241 ggctttgacc atcgggccgg ccaaagccat gtccatgctg gcggcatgct tcgccttcag
      301 cacgcccatg accttgccca tgtccttgat gctggccgcg ccggtctgct tgaccgcatc
      361 ggcgaccgcc tgctccatcg ccgccgcatc catctgtgac ggcaggaagc tctcgatgac
      421 ggcgatctcg gcttcttcct tggcggcaag ttccgggcgg ttgcccttgt tgtagagttc
      481 caccgattcc cgccgggact tcaccatgcc ccgcagcatg gcgatgatct cctcatccgg
      541 caccttgtcg atgcccttcg gacgagacgc gatgtcgatg tccttcagcc tggccatgac
      601 catgcgcagg gtcgaggttc tggcggcgtt tcccgccttc atcgacagct tcacctgttc
      661 agtgaatgtt tctctcagcc ccatggcggc actccgacgg ataattggtc gtatactata
      721 gatactgccg ccgcgtcagg cggaagtcgg ggtcggtggg cgccatgtgt ccggcgtttc
      781 ctcgtccgga tgctggcggc tgatgacgac tcgcgtcgcg ctgccgcttt cggtagccgg
      841 cgtgtccggc aaaacacctg gtgcgaccag gtgaatccac cccagacgcg cctccacgcc
      901 atagttcttt gtcggaggcg ccagttcggg cgtgtccagg ctggcggtgg tcacctcgat
      961 cgcttctccg tcgaggtagc ggaaggtcag cggcgtgccg caggcggcgc agaaccgccg
     1021 ttccgccacc gatgaactgc ggtagctggc ggtctctcct cgggtccaga cgaaacggtc
     1081 cagcggcacc tttgaaaacg cggcgaacgg accgccgacc gccttctggc acatgcggca
     1141 atgacacacg ctggattcgg gcgtctcgta cagcgcgtaa cgcagcgcgc cgcactggca
     1201 gccaccggtc atcgcgggtt tggttttgta catcgtgcgc tccttccgga aaacagttcc
     1261 cgggctgtcc gacgatcggt tgcggtggga agtttcttcc catataatgt tggtcatgcc
     1321 gatctcgacc gatcatatca tagaacgcct tggtggcgcc gacgcagccg cgcgcctcac
     1381 cggagtggga acggaggctg tgcgcaagtg gcgccaaagc cgatcgatcc cttcccgcca
     1441 ttggccggcg gtggtagcgg ccaccggatt gacgatggcc gacctgcaat cttctgacca
     1501 agcgagtgcg ccaatgtccg atgcgaccac ggttcccccc gccggcgcca ccgcggtgtt
     1561 ggtgctggcg gatggcaccg agttctgggg gcgtggtttc ggcgcgcata catcgcgaac
     1621 tggcctcggt gtgcatacgc cccgaccgcg ggaggcgacc catcctgatg atcgcgcgcg
     1681 ggcagccggc aaagtggggg aggtctgctt cagcaccggc atgaccggat accaggaaac
     1741 cctgaccgac ccatcattcg ccggccagat catcaccttc acctttcccc atatcggcaa
     1801 tgtcggcacc aaccgcgacg atatcgaggc gactaccatc gccgcccgtg gtctcgtggt
     1861 gaagcaggac atcaccgaac catccaactg gcgctcgacc cagacgctgc aatcctggct
     1921 gcgcgcccag ggtgtgactg gaatcgccgg ggtggacacc agaacactga cg
//
LOCUS       DASWQN010000515         3755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1896665, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000515 DASWQN010000000
VERSION     DASWQN010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896665"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VGC82_08585"
     CDS             complement(<1..848)
                     /locus_tag="VGC82_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated
                     beta-propeller protein"
                     /protein_id="HEY0423365.1"
                     /translation="MQTVNFHRMLRLAAVTLVTVLVRPAGAYMAYVTNERDNTMSVID
                     LDQMKTVKTVPVGQRPRGITMTNDGTEIFVCASDDDTIQVIDPKTLRIVGDLPSGPDP
                     ETFALHNSGNPLYVSNENDNMVTVIDVPTHKMIAQIPTGVEPEGMAVSPDGKTFVNTS
                     ETTNMAHFFDVASRKMVASVAVGARPRIAQYKHDGSEVWVSSELGGTVSVVDPRQHQV
                     THKISFEIQGLRAESVQPVGIRFAPDDATAFVALGPANRVAVIDTKTYAVRKYLLVGQ
                     RVWQMA"
     gene            complement(836..1894)
                     /locus_tag="VGC82_08590"
     CDS             complement(836..1894)
                     /locus_tag="VGC82_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0423366.1"
                     /translation="MLDKPAADDGWAGAQLANADNNTTGGFINQQFELTSTLLRAGDD
                     PAGALAKAAEQGIALVLTDVPAAPLLGLATAAQTKGLTVFNIAAPDDALRQASCLANM
                     IHVAPSRAMLTDALAQYLVWKKWTRWVLAYGSHPEYTALADAYRRSARRFGARILKEV
                     EYKDTGGARQTDSGVVQTQQQMPVFTQGFPDHDVLVTADENEVFAGYLPFRTWDARPV
                     VGSAGLRPVSWSPSSESWGGAQLQDRFVRSARRRMTPLDMQAWTACRMIGEAATRSGS
                     MDPAKIMQYMKGPDFGIGAYKGQKLTLRDWDLQLRQPILLDDGQSVVSISPQPGFLHQ
                     VTELDTLGFDRPETRCKL"
     gene            2138..2644
                     /locus_tag="VGC82_08595"
     CDS             2138..2644
                     /locus_tag="VGC82_08595"
                     /inference="COORDINATES: protein motif:HMM:NF023113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3280 domain-containing protein"
                     /protein_id="HEY0423367.1"
                     /translation="MRTWSYGMLMCLGLATTGTRAAEPSPTAIFGFELHDTSLQGEKQ
                     GPRADEQQRLGALDTELRDALTRSGCCTVVDLGPVALQSRGMDMRTCDGCDVELARKT
                     AAKISVTGWVQKVSNLILNINVVAREVGTDKVIEAGSVDIRGNTDESWSRGLSYLLRQ
                     RLHPSDWR"
     gene            2666..2878
                     /locus_tag="VGC82_08600"
     CDS             2666..2878
                     /locus_tag="VGC82_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423368.1"
                     /translation="MNLVDLILLACTLANPSACREFHVLFQSSGSLRSCMMQAQPYMA
                     QWAGEHPTFRIARWRCAWPDQEDGKI"
     gene            2960..3349
                     /locus_tag="VGC82_08605"
     CDS             2960..3349
                     /locus_tag="VGC82_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEY0423369.1"
                     /translation="MVRSYLIASAIFVALACPAKAQDAAAGEKDFAVCRACHQIGPNA
                     KIAVGPVLNGVVGRKAGTYPGFAYSPANKDSGIVWTPEELDKYLTNPQAVVPHTKMIY
                     PGQKDEQKRKNLIAYLQQFNEDGSKKN"
     gene            complement(3566..3642)
                     /locus_tag="VGC82_08610"
     tRNA            complement(3566..3642)
                     /locus_tag="VGC82_08610"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3606..3608),aa:Ile,seq:cat)
BASE COUNT          722 a         1131 c         1170 g          732 t
ORIGIN      
        1 aacgccatct gccagacgcg ctgcccgacc aacagatact ttctcacggc ataggttttg
       61 gtatcgatga cggcgacgcg attggccgga cccagtgcga cgaacgccgt cgcatcgtcc
      121 ggggcaaatc gaatgcccac gggctgcacc gattcggccc gaagcccctg gatctcgaac
      181 gagatcttgt gcgtgacctg atgctgtcgc ggatccacga cgctgacggt gccgcccagc
      241 tccgaggata cccagacttc cgacccatca tgcttgtact gggcaatgcg cggccgagcg
      301 cccaccgcaa cgctggcgac catcttgcgg ctggcgacgt cgaagaaatg cgccatgttg
      361 gtcgtctcgg acgtgttgac gaaggtctta ccgtcgggac tgaccgccat gccttccggc
      421 tccacgccgg tggggatctg cgcgatcatc ttatgcgtcg gcacgtcgat gaccgttacc
      481 atgttgtcgt tttcgttcga cacataaagc gggttgcccg agttgtgcag tgcgaatgtc
      541 tccggatcgg ggccggaggg caggtcgccg acgatacgca gcgttttcgg gtcaatgacc
      601 tggattgtgt cgtcgtcact ggcgcagacg aaaatctcgg tgccgtcatt ggtcatcgtg
      661 atgccccgcg gccgctgacc cacgggtacg gtcttcaccg tcttcatctg atcgagatcg
      721 atgacgctca tggtgttgtc gcgctcgttt gtcacgtagg ccatgtaggc cccagcgggt
      781 cgtaccagaa cggtcaccag agtgacagcg gcgagacgca gcatccggtg aaagttcaca
      841 gtttgcaccg cgtttccggc cggtcgaacc ccagcgtgtc cagctccgtc acctgatgca
      901 ggaatcccgg ctgcggcgaa atcgacacca ctgactggcc atcgtccagc aggatcggct
      961 ggcgaagctg caaatcccaa tcccgcaacg ttagcttctg gcccttgtaa gcgccgatgc
     1021 cgaaatcggg gcctttcatg tactgcatga ttttcgcagg atccatcgaa ccactgcggg
     1081 tcgcggcttc accgatcatg cggcaagcgg tccaggcctg catgtcgagc ggcgtcatgc
     1141 ggcgacgagc cgaccgcacg aagcggtcct gaagctgcgc acccccccac gattcgctgc
     1201 tcggactcca ggagaccggg cgtaaaccag ccgaacccac gaccggccgc gcatcccagg
     1261 tgcgaaacgg caggtaaccg gcgaatacct cgttctcatc ggccgtcacc aggacatcgt
     1321 ggtcgggaaa cccttgggtg aataccggca tttgctgctg cgtctgcacc acgccggagt
     1381 cggtctgccg ggcgcctccg gtgtccttat attccacttc tttcagtatg cgtgcgccga
     1441 accgtctcgc ggatcgtcgg taagcatccg caagtgcggt gtactcggga tgggagccgt
     1501 aggccagcac ccagcgcgtc catttcttcc acaccaggta ctgcgccagt gcgtcggtca
     1561 gcatggctcg ggacggtgcg acatgtatca tgttggcgag acatgaagct tgccgcaatg
     1621 catcatccgg cgcggcgatg ttgaaaaccg ttaggccctt ggtttgggca gccgtggcga
     1681 gaccgagcag cggcgccgcc ggcacatccg taaggaccag ggcgattccc tgttcagccg
     1741 ccttggcaag agcaccggcg ggatcatccc cggcacgaag cagcgtgctc gtcagctcga
     1801 actgctggtt gataaagccg cccgtggtgt tgttgtcggc gttagcgagc tgcgcacccg
     1861 cccagccatc atccgccgcc ggtttatcaa gcaatgagat ggtcgggcgt cgctcggccc
     1921 atcggatgta cgctatccca acggacgctt cgtcagcctg tgcttcggat cgtgttgtcg
     1981 ccaggccggc aaccgccgca ctcgtgatca gcgcacggcg gcttaggccg tgaccgagcg
     2041 gcggaattgg acgtttcatc ctcgcagtat gaagaaactc ccctgcggcg acaacacggc
     2101 gtgccggcga gcaaccttgg agaagagagt gtcgagtatg aggacgtggt catatggcat
     2161 gttgatgtgc ctggggctcg cgacaacggg cacccgggcg gccgaaccgt cgccgaccgc
     2221 gatattcggc ttcgaacttc acgataccag cctgcaaggt gaaaagcagg gtcctcgcgc
     2281 tgacgagcag cagcgtctgg gcgcgctgga tacggagctt cgagatgcgc taacccgttc
     2341 cggctgctgt acggtggtgg atcttggtcc cgtcgcgctg caatcgcgcg ggatggacat
     2401 gcggacctgt gacggctgtg acgtcgagct ggcccgaaag acagcggcga aaatttctgt
     2461 caccggttgg gtgcaaaagg tctccaacct gattctcaat atcaatgtcg tggcacgcga
     2521 ggtcggaacc gacaaggtga tcgaagccgg cagcgtggat atccgcggca acaccgacga
     2581 atcctggtca cgcggcctgt cgtatctgtt gcgacaaagg ttgcatccgt cggattggcg
     2641 ataatcgatc tgaggaggaa agccgatgaa cctggtcgat cttatcctgc tggcgtgcac
     2701 cttggcgaac ccaagcgcct gccgcgaatt ccatgtgctt tttcaatcgt ccggctcgct
     2761 gcgaagctgc atgatgcagg ctcagccgta tatggcgcag tgggccggag aacatcccac
     2821 gttccggatc gcacgctggc gatgcgcttg gccggatcaa gaagacggca agatttgaaa
     2881 aacccttgtc aacggcattg acacaatgac gacacgtatt gaacgtcgcg cccacgtctg
     2941 agatgcctag ggaggttttg tggttcgttc atatcttatt gcgtctgcga ttttcgttgc
     3001 tctggcctgt ccggcgaaag cgcaggatgc ggcggccggc gagaaggact ttgctgtctg
     3061 tcgggcgtgc catcaaattg gtccaaacgc gaagatcgcg gtgggtccgg tcctgaacgg
     3121 cgtggtcggc cgcaaggcgg gaacctatcc cggctttgcc tattcgcccg ccaacaagga
     3181 ttccgggatc gtctggaccc cggaggaact cgacaaatac ctgacgaatc ctcaggcggt
     3241 cgtgcctcac accaagatga tctaccctgg tcaaaaggat gagcagaagc gcaagaatct
     3301 aattgcatat ctgcaacagt tcaacgagga cggttcgaag aaaaattgat gcaggatccg
     3361 ggggccgtgc gtggggcggc catccataag tgtcttccgg gcagggggaa gtcctggatc
     3421 gtcggcataa ggccggccaa gccacgagcg gaggaggccg cgcaacggcg gttaatcggg
     3481 cgcacgggtc ggcgcacgtc atgatgtgct gggctgcatt cccgcctgga cgcggcattt
     3541 gccagcgaag gcttggtagc cccaatggtg cgcccggcag gactcgaacc tgcaaccgag
     3601 cggttatgag ccgccagctc taaccaattg agctacaggc gcgtaccatg gtccttgatg
     3661 cctgcggggg tccttgatac ccgcgggggc ccttaatgcc cgcaggttcc gttccgcgtc
     3721 aacgccgctg tcccatgctg gccacggcca aggcc
//
LOCUS       DASWQN010000516         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1896847, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000516 DASWQN010000000
VERSION     DASWQN010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896847"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..374
                     /locus_tag="VGC82_08615"
     CDS             180..374
                     /locus_tag="VGC82_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0423370.1"
                     /translation="MTAHPDEYLDAIVDRMMVTNVAQIPVISRSDSQLAGYVGWKDLL
                     RVRSKSQAEVTQRVVFYRVR"
     gene            complement(438..1037)
                     /locus_tag="VGC82_08620"
     CDS             complement(438..1037)
                     /locus_tag="VGC82_08620"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY0423371.1"
                     /translation="MVALVERPVREAPESIDGRLQQNLRRLRREHGMTLDVLSRRAGV
                     SRAMISKIERGTAIPTATILGKLSAAFGIGLSQFVGDTRPRYPVLLPQIEQVIYRDPE
                     SGLERRSLSPLFPDRSVDFVLNILPAGRRVVFPGHHQGVEEYLFVSRGELIVVADGQC
                     YSVRQGSTLFYPGHSVHEFRNNTDQEVEFFILIDDTRSR"
     gene            1283..2554
                     /locus_tag="VGC82_08625"
     CDS             1283..2554
                     /locus_tag="VGC82_08625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423372.1"
                     /translation="MPDDVGASLSHAQVRSIESRGSWIAAGVTLAILSVAYGSTLLIV
                     VGMRAMELDLGMPRSTLALAGALTWVGTGLGGMVMGWLADRIGVRNSVLMGAVMVALG
                     LALSSLGTIWALYVGQGVLIGFLGMGAIYPPLLIYVSRWFDRRRGTAIALISSGQYIA
                     GVAWPALFEWLIAGPGWRITYLGFAAVVLVGVVPLAALFLRPAPAGVETGVRSTGNKR
                     VAAASVLGLRPNTVQAIICAAGFCCCVPMAIPQAHLVAFCGDIGIGAATGAMMLSVLL
                     GCAFISRQFWGAFADRHGGLKTVLAGAAFQAIAVGGFLLTQNEAGLFIVAAGYGFGFS
                     GIIPAYVVAIRDLFPSAEASWRIPLVLFTAMSGMAFGSWFAGLLYDHFGFYAPAFGSG
                     VAFNVINLLLVGMLILRQARWGRGSTAPIIA"
BASE COUNT          468 a          843 c          891 g          587 t
ORIGIN      
        1 ccggcttccg cccagcaaac agcgatgaag cagtcacttt ggcgtcgtaa tgaaccccac
       61 agaattgcat gctctcgcgg gttctcggca ttgtaaatcc gcccgccgtg ctcgcttggc
      121 ggcgcgcgag caagcatcgc caaaccacgc tacagcagct gcttacgggg taacggctgg
      181 tgacggccca tcccgacgaa tatctggatg ccatcgtgga tcggatgatg gtgacgaacg
      241 tcgcgcagat cccggtgatc tcgcgcagcg attcccaact ggccggttat gtcggctgga
      301 aggatctgct tcgcgtccgc agcaagtctc aggcagaggt aacacagcgg gttgtgttct
      361 atcgcgtccg ctgatgcagc ggcggcatca tcgaacacct cgacgctgat cgagggacgc
      421 acaccaaggc tgtaccgtca tcgcgatcgc gtatcgtcta tgaggatgaa gaactcgacc
      481 tcctgatccg tgttattgcg aaactcatgc accgagtggc ctgggtagaa caaggtgctg
      541 ccttgcctga ccgagtagca ttggccatcc gccacgacga tcagttcgcc acggctgacg
      601 aacaggtatt cctcgacgcc ttggtggtgg cccgggaaga cgacacgcct tccggccggg
      661 agaatattga gcacgaagtc cactgaccga tcgggaaaca acggagagag cgaacgccgc
      721 tccagaccag actcgggatc gcggtagatg acctgttcga tctgcggcag cagcacaggg
      781 tagcggggcc gcgtatcacc gacgaattgc gataatccaa tcccgaatgc tgccgagagc
      841 ttgcccagaa tcgtagcggt ggggatagcg gtcccgcgct cgatctttga aatcatcgcc
      901 ctgctgaccc cggcacgccg cgagagcacg tccagagtca taccgtgctc acgccgcagc
      961 cggcgcagat tctgttgcag ccgtccatcg atggactcgg gcgcctcccg gaccggacgt
     1021 tcaactaacg cgaccacctt tagatgcctg cctccgtgca gcgcggatca cacgtaacag
     1081 cctttatacc gcggcagcat tgcatcttac aacggcgttt tctacgatag tagaaagtcc
     1141 ttgggaggga cgtgatgctt tgcttcgcca ccccggttca atgcgtaaat gtttttcggc
     1201 cgcttctgcg cgatcctgat ttgacctggt cggcgccgat cggtcgtcag tctccatgcc
     1261 cttggcggcg gggaagcgcc tgatgcctga cgatgtcggc gcgtcgctgt cgcacgcgca
     1321 ggtgcgctcg atcgagagcc gtggctcatg gatcgctgcc ggtgtgacgc tggcgattct
     1381 ttctgtggcg tatggctcga ccttgttgat tgtggtcgga atgcgcgcga tggagctcga
     1441 tcttggcatg ccgcggtcga ccctggcgct cgcgggcgcg ctgacctggg tcgggacggg
     1501 tctcggcggg atggtcatgg gctggctggc cgaccggatc ggtgtgcgca acagtgtgct
     1561 gatgggtgcg gtgatggtcg cgctcggtct cgcgttgtca tcgctcggca cgatctgggc
     1621 gctctatgtt ggacaaggcg tcctgatcgg gtttctcggg atgggcgcga tctatccgcc
     1681 gctgctcatc tatgtcagtc gctggttcga tcggcgtcgc ggcaccgcca tcgcgctgat
     1741 ttcatccgga cagtatattg ccggcgtcgc ctggccggcg ctgttcgaat ggctgattgc
     1801 cggtcctggc tggcgcatca cgtatctggg attcgccgcc gtcgtgctgg tgggcgtggt
     1861 gccgcttgcc gccctgttcc tgcgccctgc gccggcgggc gttgagaccg gggtcagaag
     1921 cacaggaaac aaacgcgttg cggcagcgag cgtgctgggg ctgcggccaa ataccgtgca
     1981 ggcaatcatt tgtgcggccg gcttttgctg ttgcgtgccg atggcgatcc cacaggcgca
     2041 tctagtggcg ttctgtggcg acatcggcat cggcgcagcg acaggcgcga tgatgctgtc
     2101 cgtcctgctg ggatgtgcct tcatctccag gcagttctgg ggcgccttcg ccgaccggca
     2161 cggcggtttg aagaccgtgc tcgcaggggc cgcctttcag gcaatagcag tcggcggctt
     2221 cctgctgacc cagaatgagg ccggactgtt cattgtagcc gccggctacg ggttcggctt
     2281 ctccggcatc attccggcct atgtcgtggc gattcgtgac ctgtttcctt cggccgaagc
     2341 gtcctggcgg atcccgctgg tgctattcac ggccatgagc ggaatggcgt tcggcagctg
     2401 gtttgccggg ctgctttacg atcacttcgg cttctacgcc ccggcattcg gctccggcgt
     2461 cgcgttcaac gtcatcaacc tgctgctggt cggaatgctg atcctgcgac aggcgcgatg
     2521 gggacgcggc agcaccgccc cgatcatcgc ctgagcacca gtatggcagg caccggcggt
     2581 ccggccatgc gtaacggcca tttccacttg gacgtgcgta ccgttaacag cttgaatacg
     2641 catctgtcag cggcctgacg accgcgatcc gattgcccac gggtggcact gctatgggcg
     2701 aatgcaccgg tgtccggcta ttctgcagag cgaagaagcg gatccgtcaa tcgtgtcggg
     2761 acgtcagata accagcgttt ttcccaccc
//
LOCUS       DASWQN010000517         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1896917, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000517 DASWQN010000000
VERSION     DASWQN010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896917"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            444..1376
                     /locus_tag="VGC82_08630"
     CDS             444..1376
                     /locus_tag="VGC82_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0423373.1"
                     /translation="MTTYLVTGGAGFIGSHLCDALIAGGHAVRVLDDLSTGQRTNLPP
                     AATLILGDVADPEIVAEALDGVDGCFHLAAIASVEKGVTDWLGTHRTNLSGTIAVLDA
                     IRRQGSRIPFVYASSAAVYGDSKTIPIAETQPCRPLSAYGADKYGCELHARVASHVHA
                     IPTTGLRFFNVYGPRQDPRSPYSGVISIFCERIAAGLPVNIFGDGRQTRGFICVADVV
                     RALLAAMALRLAGSPVFNVCTGVPTSVKDLASLIADLADQPLDVRTLACRSGEIRDSL
                     GLPDLADSVLGMPGRIELRIGLRQLLAGMAEPAA"
     gene            1412..>1727
                     /locus_tag="VGC82_08635"
     CDS             1412..>1727
                     /locus_tag="VGC82_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423374.1"
                     /translation="MPPTGFVVLTGIGLFLWGTRRRTGKFLAWASLIGLTLFGMPAVS
                     HNLLLALESGLPMTPPAGQPPQAIVVLGGEVIRARDENRGIRPGLLTLDRLRTAAALH
                     RRT"
BASE COUNT          294 a          613 c          502 g          318 t
ORIGIN      
        1 ggacgcgcta caccgggacg gtgccggacg attgccgcat gcatgccggc agtgcaaatc
       61 cgacgcaaaa acgctcttga tcacccgccg cgcagcggcc gaagtcgatg gatccgatct
      121 tgaacagcga gactggatct cgttctccgt ggctccgtgt tgcgctctgt gccatccgtg
      181 tcgaagtgca atggcaaccg ccgcacgaac ctccaggtca gccgcccgat acaccagcac
      241 agaaatcgcg atttccaaac ggaacattcc gcgcttccat ggcgcatggc caacgtggca
      301 tactgtccgc gtaaaaattg cgttcaggat acgtctgttt tgaccaaatg cccgacggcc
      361 gcctttgatc catcgccgcc tccgaaaaca acgcggaggc catcaggcgg tcgggccagt
      421 caaggaaccg aaggagccgc cgcttgacca cctatctggt gacggggggc gccggcttta
      481 tcggctccca tctatgcgat gccctgattg ccggaggcca tgcggtccgc gttctggatg
      541 acttgtccac cggccagcgg accaacctcc cgcctgccgc aaccctgatc ctgggcgatg
      601 tcgcagatcc cgaaatcgtc gcggaagccc tggacggagt cgatggctgc tttcatctcg
      661 ccgccatcgc ctcggtcgaa aaaggcgtca ccgattggct cggcacccac cgcaccaacc
      721 tcagcggcac catcgccgtg ctcgatgcca tccgccgcca gggcagccgc atcccctttg
      781 tctatgcatc gtccgccgcc gtttatggcg attccaagac cattcctatc gccgaaaccc
      841 agccatgcag gccgctatcg gcgtacggcg cggacaaata cgggtgcgaa ctgcacgcgc
      901 gtgtcgccag ccacgtccac gcgatcccca ccaccggctt gcggtttttc aacgtttatg
      961 gcccacgcca ggaccccaga tcaccctatt ccggtgtcat ctccattttc tgcgaacgca
     1021 tcgccgcagg ccttccggtc aacatcttcg gcgacggccg gcagacccgg ggtttcatct
     1081 gtgtcgccga tgtcgtgcgc gccctgctcg ccgccatggc cctacggcta gccggctccc
     1141 cggttttcaa cgtctgcacc ggtgttccta cttcggtaaa ggacctggcc agcctgatcg
     1201 ccgacctggc tgaccagccg ctcgatgtcc ggacacttgc gtgccgaagt ggcgagatca
     1261 gggactccct gggcctaccc gatttggccg atagcgtcct tggcatgccg ggacggatcg
     1321 agctgcgcat cggcctacgc cagttgctgg ccggaatggc cgaaccggcc gcgtagccgg
     1381 cgctatgcga gctttcctgg tgaccctggc aatgccgccc accggcttcg tcgtcctgac
     1441 cggcatcggc ctgttcctgt ggggaaccag gcgtcgaacc ggcaaattcc tggcctgggc
     1501 gtcgctgatc gggctgaccc tgtttggcat gcccgccgtg tcgcacaatc tgctgctggc
     1561 tctggaatcc ggcctgccca tgaccccgcc ggccggccag ccacctcaag cgatcgtcgt
     1621 actcggcggc gaggtcatcc gcgcccgcga cgagaaccgt ggcatccggc cgggactgct
     1681 gacactggac cggctgcgca cggccgccgc gctgcatcgg cgcaccg
//
LOCUS       DASWQN010000518         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1632877, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000518 DASWQN010000000
VERSION     DASWQN010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632877"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1566)
                     /locus_tag="VGC82_08640"
     CDS             complement(<1..1566)
                     /locus_tag="VGC82_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0423375.1"
                     /translation="MEWPLLSVGLNQPEDIVAARYRARQIAALVGLDASSQARMVASV
                     SEIARNAIAYAGGGRVAFAITRGQPMTLCVRVSDNGPGFADLDAVLDGRTAGEGILSA
                     RRLMDDFAIEIGKTGTTVTLSRRLANGAVFSAADIARIVGALGTSPAPSPVAELQTQT
                     TELLATLTELRTRQEELERLNVELEDTNRGVVALYAELDERAEQLRQASELKSRFLSN
                     MSHEFRTPLNSVLALSRLLLDRADGPLTAEQERQVTYIRGSAETLAELVNDLLDLAKV
                     EAGKLEIRPQYFTITELFGALRGVMKPLRQNEAVELIFEEPLDCPPLFTDEAKVAQIL
                     RNLISNALKFTERGEVRVSAAFDAETRQYSLEVVDTGIGIAPEDHELVFQEFSQVANL
                     LQSRAKGTGLGLPLSRRLAELLGGSLTVRSTKGAGSAFRLCIPSSMGPRSAGLPGQIS
                     TGPPEVVSQPGQSRGVLVIDDEETSRYVLRQMLGCCGPLRVQEAQTGAEGLRLARSWH
                     PDAVLLDLCLPDID"
     gene            complement(1569..2555)
                     /locus_tag="VGC82_08645"
     CDS             complement(1569..2555)
                     /locus_tag="VGC82_08645"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding SpoIIE family protein phosphatase"
                     /protein_id="HEY0423376.1"
                     /translation="MEATIVREPTDVAAARRQIGGLAARVGYDATRAGRVAIVVTELA
                     QNVLRHGGGGEILAGIDPERPAGIQIIALDRGPGMADVKACLRDGYSTGGTSGNGLGA
                     VKRLAHEVLIHSAPGHGTAVLVRMGGDSEPRSGTTRTTAALCIPVTGEAICGDTAAIV
                     SRADGSVGILLADGLGHGPLAAAASQEAARLFRKHPEGSPTAVLASLHAGLRATRGAA
                     VAVASIDPAARQVVYSGIGNIAGFITDTGGVRRMVSHSGTAGHTAGRLQDFRYPIHHQ
                     PVLAMYSDGLTTSWSPDAHPGLFGLDPLLIAAILYRDHARGRDDASVVVWKG"
BASE COUNT          491 a          904 c          861 g          470 t
ORIGIN      
        1 gtcgatatcg gggaggcaaa ggtccagcag caccgcatcc gggtgccagg accgcgccag
       61 ccgcagtcct tcggcgccgg tctgggcctc ctggacgcga agcgggccac agcaaccgag
      121 catttggcgc agcacgtagc gggatgtttc ttcatcatcg ataaccagaa caccgcgcga
      181 ctgacccggt tgcgacacga cctccggagg ccccgtcgaa atctgaccag gcaaacccgc
      241 cgaacgcggc cccatcgagc ttggaataca cagccggaat gccgaacccg ctcccttggt
      301 gctgcgcacc gtcagcgacc cgccaagcag ttcggccagc cggcgcgaca gcggcagccc
      361 gaggccggtg cccttggcgc gggattgcag caggttggcg acctgggaaa attcctggaa
      421 caccagttcg tggtcctcgg gggcgatgcc gatgccggta tcgacgacct ccaggctgta
      481 ttgccgcgtt tcggcgtcga acgccgcgct gacgcgaacc tccccgcgtt cggtgaactt
      541 gagcgcgttg gagatcagat tgcgcaggat ctgggctacc ttggcctcat ccgtgaacag
      601 cggcgggcag tcgaggggtt cctcgaagat cagttccacc gcttcgtttt gccgcagcgg
      661 cttcatcacg ccgcgcagtg cgccaaaaag ttccgtaatc gtaaagtatt gcggcctgat
      721 ttccagcttg cctgcttcga ccttcgccag atccagcagg tcgttgacca gttcggcaag
      781 ggtttcagcc gacccgcgta tatacgtaac ctggcgctcc tgctccgccg tcagcggccc
      841 atcggcgcga tccagcaaca gccgcgacaa ggccaggacg gagttcaggg gcgtgcggaa
      901 ctcgtggctc atgttcgaca ggaagcgcga cttgagctcg ctggcctggc gcagttgttc
      961 cgcccgttcg tccagttccg cgtacagcgc gacgacgccg cggttggtat cttccaactc
     1021 gacattcaac cgctcaagct cctcctggcg ggtgcgcagc tccgtcaggg tcgcgagcag
     1081 ttcagtcgtc tgggtctgca attcggcgac tgggctgggt gcggggctgg taccaagcgc
     1141 gccgacgatc cgggcgatat cggcggcgga gaaaacagcg ccattcgcaa ggcggcggga
     1201 aagcgtgacg gtggtgccgg ttttaccgat ctcgatggca aaatcgtcca tcaggcgcct
     1261 tgcgctaagg atgccttcgc cggcggtgcg gccatccagc acggcatcca ggtcggcgaa
     1321 gccggggccg ttgtcggaca cacgaacgca caatgtcatg ggctgcccgc gggtgatcgc
     1381 aaaggcgacc cgtccgccgc cggcataagc gatcgcattg cgcgcgattt ccgaaaccga
     1441 ggcgaccatc ctggcctggc tggatgcatc caggccgaca agagcggcaa tttggcgtgc
     1501 gcggtaccga gcggcgacaa tgtcctcggg ctggttcagg ccaacgctta gaagtggcca
     1561 ttccatggtt agcccttcca gacaaccacg gacgcgtcat cgcgcccgcg cgcgtgatcg
     1621 cgatagagta tagcagcaat cagcaatggg tccaaaccaa atagaccggg atgagcgtcg
     1681 ggcgaccagg acgtggtcag gccatccgag tacatggcca ggaccggttg gtggtggatc
     1741 ggataacgga aatcctgcag gcgaccggcc gtgtgtcccg ctgtgccgct gtgcgacacc
     1801 attcggcgga ccccgcccgt gtcggtgatg aacccggcga tgttgccgat gccgctgtaa
     1861 acgacctgtc tcgcggccgg gtcgatcgac gccaccgcca ccgcggcgcc gcgcgttgcc
     1921 cgcaacccgg cgtgcaggct ggccagaacc gcggtgggcg agccctcggg atgtttgcgg
     1981 aacaaccgcg cggcttcctg tgatgcggcg gcggcaagcg gaccgtgccc caggccgtcg
     2041 gccaacagga ttccaacact gccatccgcc cgactgacga tggcggcggt atcgccgcag
     2101 atcgcttccc ccgtcacagg gatgcataat gcggcggtgg tgcgcgtggt gcccgacctc
     2161 ggttcgctat ccccgcccat ccgcacgagc acagcagtgc catggcctgg ggcggagtga
     2221 ataagcacct catgcgccag ccgcttgacc gcacccaggc cgttgccact ggtgccgcct
     2281 gtcgagtagc cgtcgcgaag gcaggccttg acatcggcca tacccggtcc tcgatcgagc
     2341 gcgataatct ggatgcccgc cggacgttcc ggatcaattc cggccagaat ttcgccgccg
     2401 ccgccatggc gaagcacatt ctgcgccagt tccgtcacca cgatggcaac ccgaccggct
     2461 cgggtcgcat catagccgac acgcgccgcc aggccgccaa tttgccgtct ggccgcagca
     2521 acatcggtcg gttcgcggac aatcgtggct tccatacatt tacttcccga ccgaggcatc
     2581 gccttcgacc aaccgagatg tcgcggcctg catcacctcc aacgtgcgat ggtgacagtc
     2641 gtgccctgcc ccggcgtcga ttttattgca aattcgttgc aaagacgctt ggcgccgcct
     2701 agaccaaggc ccatgccttt gccgct
//
LOCUS       DASWQN010000519         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_313647, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000519 DASWQN010000000
VERSION     DASWQN010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313647"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..781
                     /locus_tag="VGC82_08650"
     CDS             215..781
                     /locus_tag="VGC82_08650"
                     /inference="COORDINATES: protein motif:HMM:NF025180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEY0423377.1"
                     /translation="MEPEPPFTPFLTLLAEIEDPRRAEGKLYRLPHVLLFAIMAIVAG
                     ANSYRTVHTFIDVHLARLRDVFRLTWRRAPAYTTIRGIIQQVDAGSVEPVFRRHAAIL
                     HEQTGSAERRQVAIDGKVLRRSFDGFHDRKAAHLLSVFATDAAVVLAHADCDEKSNEI
                     PALQTLLSELGLAGALMTVDALHCQKNI"
     gene            750..1355
                     /locus_tag="VGC82_08655"
     CDS             750..1355
                     /locus_tag="VGC82_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEY0423378.1"
                     /translation="MTPCTVKKTFEQVAAAGAHLIAQVKANQPTLHQRIAEICQSAAP
                     VDQVRTTDQKRRSRDEIRLVEVFDPGAAACLDAAAEWADHVTAIVRVSRDTLKRITTT
                     GLWQGTSQVAYFIADTAVPASVCAQAIRGHWGIENRLHYVRDVSFREDDSRIRCNPGI
                     FARLRSFAANILRFNSVQNMSNGRYRIAVGGVDAMLSLRVM"
BASE COUNT          297 a          537 c          474 g          281 t
ORIGIN      
        1 gggaggatgg agctgagaac ctccttcgcg gtgtcgacga gcatgctgcc ctcgtccggc
       61 acggctgttc aacgctctct acttgagggg tgaagcgagt cgaaaatggc acagagggcg
      121 gggataggtg cgggtgggca caggaagggg tttcgaccgg cccacttctc tgacgctgtt
      181 cctggcaacc tgagttccac cgaacgaggt tttcatggag ccggagccgc cgttcacgcc
      241 gtttctcacg ttgctggccg agatcgagga tcctcggcgg gctgaaggca agctgtatcg
      301 cctcccgcac gtcctgctgt tcgccatcat ggcgattgtc gccggggcga actcgtaccg
      361 caccgtccac accttcatcg acgtccatct tgcccggctg cgcgatgtct tccgcctgac
      421 gtggcgacgc gctccggcgt acacgacgat ccgagggatc atccagcagg tggacgcggg
      481 ctcggttgag ccggtcttcc ggcgccatgc cgccatcctg cacgagcaga ccggctcggc
      541 ggagcggcgt caggtggcga tcgacggcaa ggtgctgcgc cggagtttcg acggcttcca
      601 cgaccgcaaa gccgcccatc tcctcagcgt ctttgccacc gatgcggccg tggtgctggc
      661 tcatgcggac tgtgacgaga agtcgaacga gatcccagcc cttcaaaccc tgctcagcga
      721 gcttggcctc gccggtgccc tcatgacggt tgacgccctg cactgtcaaa aaaacatttg
      781 agcaggttgc cgcggccggt gcccacctca tcgcccaggt caaagccaac cagcccaccc
      841 tacaccagcg cattgccgag atctgccaga gcgcggcacc ggtggatcag gtccggacca
      901 cggatcagaa gcggcgatcg cgcgacgaaa tccgcttggt cgaggtcttc gatcccggcg
      961 ccgccgcctg cctggacgcc gccgccgaat gggccgatca cgtcacggcg attgtccgcg
     1021 tctcccgcga cacgctcaag cgcatcacga cgaccggctt gtggcaggga accagccagg
     1081 tcgcgtattt catcgccgac accgctgtgc ccgccagcgt ctgtgcccaa gccatccgcg
     1141 gccattgggg cattgaaaac cgactgcact atgtccggga tgtcagcttc cgtgaggacg
     1201 acagccggat ccgctgcaat cccggcatct tcgcccgcct gcgatccttc gccgccaaca
     1261 ttctacgctt caacagcgta caaaacatga gcaatgggcg ctatcgcatc gccgtcggcg
     1321 gcgttgatgc aatgctctcg ctgcgcgtca tgtaaagagc gttgaacagc cctgatccac
     1381 acctcgcctc cgccaggaaa cttcctactt cgtcaacacc accgggcagc cacccgccta
     1441 aaagtcgacg ctaaggcgac ctgcttcgcg ttcctcacgg agaaagagaa acggacttgc
     1501 gacgcgcgag ggaaagcgca ccattgaccc acctaaggct caccgtcgga tcccatcccg
     1561 ttgatcgaag ccgtcgaact tagggggcg
//
LOCUS       DASWQN010000520         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1633201, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000520 DASWQN010000000
VERSION     DASWQN010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633201"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..782)
                     /locus_tag="VGC82_08660"
     CDS             complement(123..782)
                     /locus_tag="VGC82_08660"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0423379.1"
                     /translation="MVDIFDEVEEDLRAERAQKLFKKYAWLIVAAIVAVVGAAAGWQL
                     WTRYQNQQDAAAAARFIAAQTELELPAVSKPDQITVLDRLAATAPEGYKTLARLRAAG
                     LKADAGDLQGAESLWNAVAADSSADSLLRDFASLMAIARELDHGDPSQLESRLKPLAD
                     YANPWSSLAREQAAMLSLRQGKIEDARKALQGLANDFRAPSGLRTRASALLAGLPHDA
                     T"
     gene            912..1280
                     /locus_tag="VGC82_08665"
     CDS             912..1280
                     /locus_tag="VGC82_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423380.1"
                     /translation="MRRAFLLSFLPLAGCGWSDNLIATALVAANVGSVATIQRSPPDA
                     IYSWWTGRDCSVVRLDQGKSYCRPTEPIPPPPAFCTRSLGTVNCWQDPESLPDHPAGV
                     ADGPAGLTAEQEANRVRRWP"
     gene            1359..1435
                     /locus_tag="VGC82_08670"
     tRNA            1359..1435
                     /locus_tag="VGC82_08670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1393..1395,aa:Arg,seq:ccg)
     gene            1552..>1811
                     /locus_tag="VGC82_08675"
     CDS             1552..>1811
                     /locus_tag="VGC82_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423381.1"
                     /translation="MSVLDGLRVVQIGPGLAAAVCGRLFADVGAAVSRIDPDRLTPLA
                     QHLNPTDGPSHPIEAAELIVCEGSPNTLRRTGRDPDSLRRINP"
BASE COUNT          316 a          563 c          610 g          322 t
ORIGIN      
        1 gagaacgaac ggagccagca gcgcggatcg gcggcccatc aacttgcggg ttttcgaagt
       61 gacattcacg ctcacgccag ggcttccagt cggtcgatag gtttggcggt catgtcgcat
      121 cctcatgtcg catcatgggg aaggccggcg agcagggccg aggctcgggt gcgcaagccg
      181 gagggtgcgc ggaaatcgtt ggccagaccc tgcagggcct tgcgggcgtc ctcgatcttg
      241 ccttgccgca agctaagcat cgccgcttgc tcgcgtgcca agctggacca gggattggcg
      301 tagtcggcca agggcttcag ccgggattca agctgcgacg gatcgccgtg atcgagttcg
      361 cgagcgatcg ccatcaggct ggcgaaatcc cgcaggagtg aatcggcgct ggagtcggcc
      421 gccaccgcgt tccacagcga ctccgcgcct tgcaggtcgc cggcgtcggc cttcaggccg
      481 gcagcgcgca accttgccag ggtcttgtag ccctccggcg ccgtagccgc caatcggtcc
      541 aggacagtga tttggtcagg cttggacacg gcgggaagct cgagttcggt ttgcgcagcg
      601 atgaagcggg cagcagcggc ggcgtcctgc tggttttgat agcgggtcca caactgccag
      661 ccggccgcgg ccccaacgac tgccacgata gcggcgacga tcagccaggc atattttttg
      721 aatagtttct gagcgcgttc cgcgcggagg tcttcctcga cctcatcgaa aatatcgacc
      781 actcgaactc tcttgcctgc ctcgatttgc cgccgcaccc aagtggcggc ggaccataac
      841 ggcgtcgggg tgtggccgta aaccgccgat gcggcaaggg ttcatttacc tttgtcgtgc
      901 aggatggcgg aatgagacga gcgttcctgt tgagtttcct gccgttggcc ggctgcggct
      961 ggtcggacaa cctgatcgcc acagccctgg tggcggcgaa cgtaggcagc gtggcgacga
     1021 tccagcggtc accgccggac gcgatatact cgtggtggac cggtcgcgac tgttcggtgg
     1081 tccggctcga ccagggcaag agctactgcc gtccgacgga gccgataccg ccgccgccgg
     1141 cattctgcac ccgcagcctg gggaccgtga attgctggca ggacccggaa agtctgccgg
     1201 accatccggc tggtgtggcc gatggtccgg cggggctgac ggcggaacag gaagcaaacc
     1261 gggtgcggcg gtggccttga cgaatatcgc cgaagggaga ttgaggtttc cagccgatgc
     1321 cggtatagga ggggcgtcgt cccgggtcgg ggcgcgcggg acccgtagct cagctggata
     1381 gagcgtcgcc ctccgaaggc gaaggtcgca cgttcgaatc gtgtcgggtc cgccaatcat
     1441 ccaggttcca gggcctcctg acggcccgcg cgaaacaagc cagtcaacgc caaggtttgt
     1501 aaaccaagcg atctctgtgc atcgtttccc ggacgatgcg gggaagctgg aatgtcggtg
     1561 ctggatggtc ttcgcgtggt gcagatcggg ccaggtttgg ccgccgcggt ctgcggacgg
     1621 cttttcgccg acgttggtgc ggcggtcagc cgaatcgatc ctgaccgctt gacgccgctg
     1681 gcccaacacc tcaatcccac cgacggcccc agccacccaa tcgaagccgc ggaactgatc
     1741 gtctgtgaag gcagccccaa caccttacgc cgaaccggcc gcgatcccga ctcactccgc
     1801 cggataaatc c
//
LOCUS       DASWQN010000521         7140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2028990, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000521 DASWQN010000000
VERSION     DASWQN010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028990"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..1123)
                     /locus_tag="VGC82_08680"
     CDS             complement(362..1123)
                     /locus_tag="VGC82_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0423382.1"
                     /translation="MRFKDKVALITAAANGIGRATAEIMAREGAVVVCIDNHSERLNA
                     AVAALGERAHGRLCNALNQSHVDQVVDGTVREFGRIDILVNAVGGSTIISKPSATVEE
                     HSLDDWQRVIAFNLDGTFLFTHAVVPVMKNQKSGKIVNLASIAGRGISVASGSAYAAA
                     KGGIIAFTRKLSFELGPYGININAIAPSLTLTERITPHWNQRPPDAQAAQIEATPLRR
                     VAVASDQAKVICFLASSDADFVTGLTIDVTGGQSG"
     gene            complement(1138..2175)
                     /locus_tag="VGC82_08685"
     CDS             complement(1138..2175)
                     /locus_tag="VGC82_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY0423383.1"
                     /translation="MKLGLSIGYSKAQLDLPVALVQRAEELGYDSVWSAEAYGSDAVT
                     PLAFLAAVTKKIRLGTGIMQLAARTPANAAMCAGTVDALAGGGRFIAGLGVSGPQIVE
                     GWYGQPWGRPYYRLKDYVAIMRKVFRRDEPVTHDGREISLPYVGEGAAGVGKPLKSIL
                     HMNPDIPIYLATGSESTVKLTAEIADGWLPMGFVPGCMEEYGPWLEEGFRRAGNGKSL
                     KDFEIQASIHVEVADDVKAALARLKPEVALYVGGMGHKDKNFHKDQMIRRGFEQAADR
                     IQELYLAHRKEEAVAAVPDEWVDMKSLVGPPERIRQRFKAWEESGATSVTVRSRQPEA
                     IEIMAQAARLN"
     gene            complement(2172..3512)
                     /locus_tag="VGC82_08690"
     CDS             complement(2172..3512)
                     /locus_tag="VGC82_08690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0423384.1"
                     /translation="MRQMVMVGFLQAQNCTNLVSSWRHPESREDSTSADYYQTIGRVL
                     EEGKFHVGFFDDRLAMPDRYGNDHRHTVEHGIRCVKMDPLTVLTVMGMATKRLGLGAT
                     RSTTYYEPFDVARTFATLDLMTEGRAAWNVVTSMNDGEALNMGKDEHLEHDSRYDRAD
                     EFMEVVLGHWNSWDDDAVVHDKKTGIFADPDKVRRLDHKGAWFKSRGPFTVPRSKQGH
                     PVIIQAGQSGRGKQFSARWGELVFVSTPVSMEHGRQTYRDLKAEVERQGRDPAHLTIT
                     PSAYIVCAETKAEAEDKMALIETLATDEDALSLLSEAMNFDFASKGLDEPFTDAELNG
                     ISGTQSMRDRVLNASGIRNPTPRDFIKFSHRARPNMPFIGGPKEVADGLEEWFSTGVC
                     DGFVIAATHVPGTYMEFVRHVVPELQRRGLYHKDYTGATLRENLGLPIPHKSLA"
     gene            complement(3523..4755)
                     /locus_tag="VGC82_08695"
     CDS             complement(3523..4755)
                     /locus_tag="VGC82_08695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0423385.1"
                     /translation="MKFGVFYEHQLPRPWTANSEYELFQNSLSQVELADKLGYDYMWE
                     VEHHFLEEYSHSSSPEVFLAAASQRTKNIRLGHGIVQLTTNQPHRVAERVATLDLISG
                     GRVELGMGEGAGPAELHPFGVRVRDKRDRWEEAVQAIIPMFTRDDWEFHGKYHDFPAR
                     NVVPKPYQKPHPPLWVACSNIRTIGEAGQWGMGALGFSFVSPEAAQAWVHKYYNMYLH
                     QPRRLADYATNPNVAIVNGFMCAPTDEEALEKAAGWTFFIFALSYYGRKGVDAPGKGD
                     LWREYQDWRHTEKAQLALRNGLIGSPETIRKRLRQFEAAHVDQVILLNQAGKTSHQDI
                     CGSLDLFAREVMPEFHDRQPAQDAWKQDVLAGRFVLEDLDTERYDLYAHQNEDIVRMT
                     PDQLKQRMAEKDRLAAAG"
     gene            complement(4884..5513)
                     /gene="gmk"
                     /locus_tag="VGC82_08700"
     CDS             complement(4884..5513)
                     /gene="gmk"
                     /locus_tag="VGC82_08700"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY0423386.1"
                     /translation="MIEIARRGICLILSAPSGAGKTAIAEALLATEPRLERSISVTTR
                     DRRPTEVDGVHYHFLTAEKFQQALRDDALLEWARVLQGTHAYGTPRAPVEKALAEGVD
                     VVFDIDWQGHRQLREKLRGDVVSVFVLPPNLAALRSRLVGRAGDHGEEIERRMRVARD
                     EIRHWVDFDHVVLNEDLPTATEAVRAVLHAARSAATRLTGLQGFIDALR"
     gene            complement(5510..6379)
                     /locus_tag="VGC82_08705"
     CDS             complement(5510..6379)
                     /locus_tag="VGC82_08705"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY0423387.1"
                     /translation="MTGFARAEGAAGGIAWAWEMRSVNGRSLELRFRLPTGWDSMEAG
                     WRDLAAKALKRGNVTANLTIKRPSETRLELDPAALEQVLTIATDLHRRIPGSPPPSAE
                     ALLSLPGVLRQAQADQQEEKAACASLVQASFVAALADLVASRQAEGDRLASILHRQLA
                     EITTLCDTATAQALDQPAIQRARMLDNLQTLLRETPNLTEERIAQEVAILASRSDVRE
                     ELDRLSSHIAAAHDLIGEAVNIGRKLDFLIQEFNREVNTLCSKSASAELTATGLRLKA
                     AIEQLREQVQNIE"
     gene            6477..7097
                     /locus_tag="VGC82_08710"
     CDS             6477..7097
                     /locus_tag="VGC82_08710"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY0423388.1"
                     /translation="MPNLDMELAAGGRVAGVDEAGRGPLAGPVVAAAVVLRADIPSEL
                     AAMLDDSKKLTAAQRLAAFLALRASSAEIGIGAASVAEILRFNILHASMLAMHRAVGR
                     LRMAPDLALVDGNYPPKLGCPVRCVVGGDGLCLSIAAASIVAKVVRDRAMVRLASRFP
                     EYGWHANVGYATALHREALRRLGPTRHHREAFGTVAQLRLALTIED"
BASE COUNT         1251 a         2289 c         2117 g         1483 t
ORIGIN      
        1 agccggacac gagagtgaga caggtttccc gttacatgcg tcagccaaaa tatatcgaac
       61 ctcaaaaatg accgcagtcg catcgaacgg cgtgtcttct gatcatgatc agtcggatcg
      121 ggtcaaacca aatcacgttg atacgacggc ggcatcttga cgctccggtc tattccgaaa
      181 tgatcgctat gggcccggtc ccatttatgg cgcggagatc caccagtgct ggatgccgct
      241 ccagcggtgc ccacgtaaag ggtggcggtt gatcatactg tgtcgtggcc gtgagatgcg
      301 ttatggccgc cttggcacgg tcacccggtg aagccttatt gctggccctg gcgcagatta
      361 cctacccgga ttgccctccc gtcacgtcga tggtcagtcc agtgacaaaa tcggcatcgg
      421 atgacgcaag gaaacatatc actttcgcct gatccgaagc gaccgccacc cgtctaaggg
      481 gggtcgcctc aatttgtgcc gcctgagcgt ccggcggccg ttggttccag tgcggtgtga
      541 tccgttcggt cagtgtcaag cttggagcga tcgcattgat gttgatcccg tatgggccca
      601 gttcgaacga gagcttccgg gtgaaggcga tgatcccccc tttggccgcg gcataggcgc
      661 tgccgctggc gacactgatg ccgcgaccgg caatcgaagc taaattgacg atcttgccgg
      721 atttctggtt tttcattacc ggcacgaccg catgcgtgaa caggaacgtt ccatccaggt
      781 tgaaggcgat cacgcgctgc caatcatcca gcgagtgctc ttcaacggtc gccgaaggtt
      841 tcgaaataat agtgctgccg ccgaccgcat tcaccaggat gtcgatgcgt ccgaactccc
      901 gcaccgtccc atcaaccacc tgatccacat gactctgatt gagcgcgttg cacagccgcc
      961 catgcgcacg ctcaccgaga gccgcaacgg ctgcgttcaa tcgttcggaa tggttgtcga
     1021 tgcagaccac aactgcgccc tcccgggcca tgatctcggc cgttgcgcgt ccgattccgt
     1081 tcgcagccgc tgtgatcagt gcgaccttgt ccttgaagcg catttgtttt tccccgctca
     1141 gttcagcctg gcagcctgtg ccatgatctc aatcgcttcg ggctggcgcg aacgcacggt
     1201 tacgctggtc gcccctgact cctcccaggc cttgaaccgc tgccgaatgc gttcgggcgg
     1261 tccaaccagc gatttcatgt ccacccactc gtcgggcacc gcggcgacag cttcctcctt
     1321 gcgatgcgcc agatacagtt cctggatgcg gtccgctgcc tgttcaaagc cgcggcggat
     1381 catctggtcc ttgtggaagt tcttgtcctt gtgccccatc cctccgacgt agagtgccac
     1441 ctccggcttc aaccgggcca aggcggcctt cacgtcgtcg gcgacttcca catggatcga
     1501 cgcctggatc tcgaaatcct tcaggctttt gccgttgccg gcgcggcgga agccttcttc
     1561 cagccagggg ccgtattctt ccatgcagcc cggcacgaat cccatcggca accacccgtc
     1621 agcaatctcc gccgttagtt tcacggtcga ttcgctgccg gttgcgaggt agatcgggat
     1681 gtccggattc atatgcagaa tcgacttgag cggtttgccg acaccggccg cgccttcgcc
     1741 cacataggga aggctgattt cgcggccgtc atgcgtcacg ggctcatcgc ggcgaaacac
     1801 cttgcgcatg atcgcgacgt agtccttcag ccggtaatat ggccggcccc acggctgccc
     1861 gtaccaacct tcgacgatct gcggccccga gacacccagg cccgcaatga aacgtccgcc
     1921 gccggccagg gcatccaccg tgcccgcaca catcgccgca ttggcgggcg tgcgcgctgc
     1981 caactgcatg atgccggtcc cgaggcggat tttcttcgtt accgccgcca ggaacgccag
     2041 tggcgtcacc gcatcggaac catacgcttc ggcgctccag accgagtcat aaccaagttc
     2101 ctcggcacgc tgcaccagtg ccaccggcag atcgagttgc gccttggaat agccgatcga
     2161 taacccgagt ttcatgccag gctcttgtga ggaatcggca acccaaggtt ttcccgcaag
     2221 gtcgctccgg tataatcctt gtgatacagt ccgcgccttt gcagctcagg cacgacgtgc
     2281 cggacgaact ccatgtatgt gcccggcaca tgggtggcgg caatcacgaa cccatcgcag
     2341 acgccggtcg aaaaccattc ctccaagcca tccgccactt ccttggggcc gcctatgaag
     2401 ggcatgtttg gacgtgcgcg atggctgaac ttgatgaaat cccgcggcgt tgggttgcgg
     2461 attccgcttg cgttcagcac ccggtcgcgc atcgactgag tgcccgaaat tccattcagt
     2521 tcggcatcgg tgaacggctc atcgagtcct ttgctcgcaa aatcgaaatt catggcttcc
     2581 gacaacaggg acaatgcgtc ctcgtccgtc gcgagggtct cgatcagcgc catcttgtct
     2641 tccgcttcgg cctttgtctc ggcacagaca atataggcag acggcgtgat cgtcagatgg
     2701 gcgggatccc gtccttggcg ttccacctcg gctttcagat cgcgataggt ctggcgcccg
     2761 tgctccatgc tgaccggtgt gctgacgaac accaactctc cccaccgcgc ggaaaactgc
     2821 ttgccgcgcc cactctggcc ggcctggatg atgacgggat ggccctgctt tgatcgaggc
     2881 acggtgaaag ggccgcgcga cttaaaccac gcccccttat gatccagccg ccgcaccttg
     2941 tccgggtcgg cgaagatccc ggtcttcttg tcgtgcacca cagcatcgtc gtcccacgag
     3001 ttccagtgcc caagcaccac ctccatgaac tcgtcggctc gatcataccg agaatcatgt
     3061 tccagatgct cgtccttgcc catgttcaac gcttcgccgt cgttcatcga cgtcaccacg
     3121 ttccaggccg ctcttccttc ggtcatcagg tcgagcgtgg cgaaagtccg cgccacgtcg
     3181 aacggttcgt aataggtcgt cgatcgcgtc gccccgaggc ccagccgctt cgtcgccatg
     3241 cccatcacgg tcagcaccgt cagcgggtcc atctttacgc agcgaatccc atgttcgacc
     3301 gtgtgccggt ggtcattgcc gtagcggtcc ggcatcgcca gccggtcgtc gaaaaagccg
     3361 acgtggaact tgccttcctc cagcaccctg ccgatggtct ggtagtagtc ggcggaagtg
     3421 gaatcctccc gagactccgg gtgtcgccac gaactcacga ggttcgtgca gttctgggct
     3481 tgcaggaagc ccaccatcac catctgccgc atgacgattc tcctaaccgg cggcagcgag
     3541 ccggtctttt tccgccatgc gctgcttgag ctggtccggc gtcattcgaa cgatatcctc
     3601 attctggtgc gcgtacaaat cgtatcgttc ggtgtcgaga tcctccagca cgaagcgacc
     3661 ggccagcacg tcctgcttcc acgcatcctg ggctggctgt cgatcatgga actccggcat
     3721 cacctcacgg gcgaacaggt ccaggcttcc gcaaatatcc tgatggctgg ttttccccgc
     3781 ctggttcagc aggatcacct gatcgacatg tgccgcctcg aactggcgga ggcgcttgcg
     3841 gatggtttcg ggcgacccga tcaatccgtt gcgcaacgcc agttgagcct tctcggtgtg
     3901 tcgccagtcc tgatattccc gccatagatc gcccttgccg ggggcatcga cacctttccg
     3961 tccgtagtag gacagcgcga agataaagaa cgtccaaccg gcggctttct ccagtgcctc
     4021 ctcgtcggtt ggagcacaca tgaagccatt gacgatcgcc acgtttggat tggtggcata
     4081 atcggccagt cgccgtggct gatgtagata catgttataa tacttgtgta cccatgcctg
     4141 cgccgcttcg ggcgaaacga agctgaaacc gagtgcgccc atgccccact ggccggcctc
     4201 gccgatggtg cggatgttgg aacatgccac ccacaacggt ggatgcggtt tctggtacgg
     4261 cttcgggacg acattccgcg ccgggaagtc gtgatatttg ccatggaatt cccagtcgtc
     4321 cctggtgaac attggaataa tggcttgcac ggcctcttcc cagcggtcgc gcttgtcgcg
     4381 cacccgtacg ccgaatggat gcagttccgc cggaccagcg ccctcgccca tgcccaattc
     4441 gacgcggccg ccggaaatca ggtccagtgt cgccacccgc tccgccacgc ggtgcggctg
     4501 gttggtggtc aactggacga tgccatgccc cagccggatg ttcttcgtcc gctgactggc
     4561 cgcggccagg aacacctcgg gcgaggaaga atgggaatac tcttccagaa agtgatgctc
     4621 gacttcccac atgtagtcgt agccaagttt gtccgcgagt tcgacctgcg aaagcgagtt
     4681 ctggaacagc tcgtactcgc tgttggcggt ccagggacgg ggcaactgat gttcgtagaa
     4741 aacaccgaat ttcatggagt ttctcccttc cggtcacgct tgcacccgct ctatcgagcg
     4801 tcaagtgtcg ctcgtttttc ccggcggttc gggatgatgg gctgacattt gcgcgggaag
     4861 ccgtcagcag cgggactggg cactcatcgc agagcgtcga taaatccctg caggccggtc
     4921 aatcgggtgg cggccgatcg tgccgcatgc agcaccgcgc gcaccgcctc ggtggctgtc
     4981 ggcaggtctt cgttcagcac cacatggtcg aaatctaccc agtggcggat ctcgtctcgt
     5041 gccacgcgca tccggcgctc gatctcctcg ccgtggtccc cagcccggcc aaccagccga
     5101 gaccgcagag ccgccagatt cggcggcagg acgaacacgc tgacgacatc cccgcgtagc
     5161 ttttcccgta gctggcggtg cccttgccag tcgatgtcga acaccacatc gacaccttca
     5221 gccagcgctt tctccaccgg tgcgcgcggc gttccatagg cgtgtgtgcc ttgcaaaacc
     5281 cgagcccatt ccagcagcgc atcgtcgcgc aaggcttgct gaaacttctc ggcggtgagg
     5341 aaatggtagt gcacgccgtc cacctcggtg ggccgacggt ctcgggtcgt gacgctgatc
     5401 gagcgctcca gtctcggttc ggtcgccaac agcgcctcgg cgatggcggt cttgccggct
     5461 ccggagggcg ccgacagaat aaggcagatg ccgcggcgcg cgatttcgat cattcgatgt
     5521 tctgcacctg ttctcgcaac tgttcgatcg cggctttgag cctcagcccc gtcgccgtca
     5581 gttcggccga tgccgatttg ctgcacaacg tgttcacctc gcggttgaac tcctggatca
     5641 gaaaatccag cttgcggccg atgttcacgg cttcgccaat caggtcatga gccgcggcaa
     5701 tatgactgga caggcgatcc agttcttccc gcacgtcgga gcggctggcg aggatcgcga
     5761 cttcctgggc aatccgctcc tccgtcaggt tgggcgtttc ccgcagcaag gtttgcaggt
     5821 tgtcgagcat ccgtgcccgc tgtatcgccg gctggtcgag ggcctgggcg gtggcggtgt
     5881 cgcacaaggt ggtgatctcc gccaactgtc ggtgcagaat gctggccagc cggtcgcctt
     5941 ccgcctgacg ggaggcgaca agatcggcca aggcggcgac gaaactggct tgaaccaagc
     6001 tcgcgcaggc ggctttttcc tcctgctgat cggcctgtgc ctgacgcagc acgcccggca
     6061 gcgacagtag cgcctcggcc gagggcggcg ggcttcccgg gatgcgccgg tgcaggtcgg
     6121 tggcgatcgt cagcacctgc tccagcgctg ccggatcaag ttccaggcga gtttcagacg
     6181 ggcgcttgat ggtcagattg gcggtgacgt tgccgcgctt cagcgctttg gctgccagat
     6241 cgcgccagcc ggcttccatg ctgtcccagc cagtgggcag gcggaagcgc agttccaagc
     6301 ttcggccatt gacgcttcgc atttcccacg cccaggcgat accgccggca gccccctcgg
     6361 cgcgagcgaa tccggtcatc gaagcaattg cgttcatagc cgcggtcata ccagcggtgc
     6421 ggcacgatgc gaagcaatag cttccgcgac tcggcacacc ctgctacgcc tttgcgatgc
     6481 caaatttgga catggaactg gctgccgggg ggcgggtcgc cggtgtggat gaggctggac
     6541 gaggcccgct tgcgggtccg gtggtcgcgg ctgccgtcgt cttgcgagcc gacattccgt
     6601 ccgaactagc cgccatgctg gacgactcga agaagctgac ggcggctcag cggctggccg
     6661 cgtttctggc gttgcgcgca tcttcggccg agatcggcat tggtgctgcg tcggtggccg
     6721 agatcctgcg gttcaacatc ctgcatgcct cgatgttggc tatgcatcgg gcggtcgggc
     6781 gtcttcgaat ggcgccggac ctggcgctgg tggacggcaa ttatccgccg aaattaggtt
     6841 gcccggtgcg ctgcgtggtg ggcggcgacg gactgtgcct gtccattgcc gcagcgtcga
     6901 tcgtcgccaa agtagtccgc gaccgcgcca tggttcggtt ggcatcaagg ttcccggaat
     6961 atggatggca tgccaatgtc ggctacgcga ctgcactgca ccgcgaagcg ctccgtcgtt
     7021 tagggcctac tcgccaccat cgagaggcgt ttggaacggt cgcccagctg cgactggctc
     7081 tgacaattga agattgacgt gacttccggc gggccgtcac gatgcggcaa ggggcgttga
//
LOCUS       DASWQN010000522         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_445597, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000522 DASWQN010000000
VERSION     DASWQN010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445597"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VGC82_08715"
     CDS             complement(<1..1035)
                     /locus_tag="VGC82_08715"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423389.1"
                     /translation="MTVAVSSSGLRALLSAEGFLRLWSIGGCVNTMRSFEVLSAALFT
                     LDVTGSGLDVAIVSAARTMPMFLLGAFSGVVTEAVNRKQVLLYGQVLTCLASALVAAL
                     AWAGMAQPWHVAAAALVAGIVWSTEMATRRRMVGECVEPRLVSRALALDTMSNSFTRL
                     LGPVFAGIIYQRFGLAGAFGFSACVYAVAAMLAAGLDYRQATRRLVLVNVPRELAEGL
                     KFVRGDLVIGGVLAVTVAMNLLGFPYAALVAPIGRMVFQVSPVLVGVLAASEAFGAFL
                     GGALLTSREPRTSGRILMVGGSLLFLACVVAMPLAPLFWVACLLLVLGGFGSAAFANM
                     QTSLIVLHAPM"
     gene            1213..>1979
                     /locus_tag="VGC82_08720"
     CDS             1213..>1979
                     /locus_tag="VGC82_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423390.1"
                     /translation="MNDTAVMTPTFDRTKEDLGNIVELGHVNVRVPDQSKAVAFYLMG
                     LGLTRDPYLMAGLDNMWVNVGRGQFHLPTRGTDVVRGTTALVMPDREALLKRLQSAKS
                     YLEGTKFSFHETDDAVETVCPWGNRIRVHAPDPARFGAMRVGMPYVEFDIPAGTDLAA
                     IAKFYKEILGGISGVSSDERGPYAWANTSAESRLIYRETTAPVPEYDAHHIQITLADF
                     SGPHKKLLERGLITEESDQHQYRFLDIVDVDTNKPLFR"
BASE COUNT          416 a          656 c          623 g          284 t
ORIGIN      
        1 catcggagcg tgcagtacaa tcagcgacgt ctgcatgttg gcgaacgccg ccgatccgaa
       61 tccgccgagc accagcaaca ggcaggcgac ccagaacagc ggcgccagcg gcatcgccac
      121 cacacatgcc aggaacagca acgacccgcc taccatcagg atgcgcccgc tcgttctcgg
      181 ctcccggctc gtcagcagag ctccgccgag aaatgcgccg aacgcctcgg acgcagcgag
      241 cacgccgacc aggacaggcg atacctggaa caccatgcgg ccgatgggtg ccaccagggc
      301 cgcgtaggga aatcccagca gattcatcgc caccgttacc gccagcacgc cgccgatcac
      361 caggtcgccg cggacaaatt tcaggccttc ggcgagttcg cggggcacgt tgaccaatac
      421 cagccggcgg gttgcctggc ggtaatcgag gccggccgcc agcattgcag cgaccgcgta
      481 aacgcaagcc gagaaaccga aggccccggc caacccgaac cgttggtaga tgatgccggc
      541 aaagacggga cccagcaggc gggtgaagga attggacatc gtgtccaacg cgagtgcccg
      601 cgataccagc ctgggctcga cgcattcgcc caccatgcgc cggcgtgtcg ccatctcggt
      661 ggaccagacg atccccgcga ccagggcggc ggctgcgacg tgccacggtt gcgccatacc
      721 agcccacgcc agtgccgcga cgagcgcgga ggccagacat gtcaggacct gtccgtacag
      781 cagcacctgc ttgcggttca cggcttcggt caccacgccg gagaatgcac cgagcaggaa
      841 catcggcatg gtgcgtgcgg cggacacgat ggccacgtcc aggcccgaac cggtcacgtc
      901 cagggtgaac agcgcggcgg acagcacctc gaacgagcgc atcgtgttga cgcagccacc
      961 gatggaccac agccggagaa agccctcggc ggaaagcagc gcgcgaaggc cgctggaaga
     1021 tacggcaaca gtcacggcgg cgcttcggtc cccggcgtgc gaagaggcgc cgttcgaaca
     1081 ggcggcggca tgacgtttcc ctgttgtcgc gtttgcgccc gatacaagca ctccgcgccg
     1141 gtctggacca gtctagaagc caggactaga atgcgggtca gcgctgcatt gagcctgaag
     1201 gggaaccgag agatgaatga taccgctgtt atgacgccga ctttcgaccg gacgaaagaa
     1261 gacctcggca atatcgtcga acttggccat gtcaatgtca gggtgccgga tcagagcaaa
     1321 gccgttgcct tttatcttat gggcctgggg ctgactcgcg atccatacct gatggccggc
     1381 ctcgacaaca tgtgggtgaa cgtcgggcgc ggccagttcc acttgccgac acgcggcacg
     1441 gatgtcgtgc ggggcaccac cgcgctggtg atgccggacc gcgaagcgct gctgaagcgt
     1501 ctgcagtcgg ccaagtcata cctggagggc acgaagttca gcttccacga gactgacgac
     1561 gccgtcgaaa cggtctgccc ctggggcaac cgcatccgcg tgcacgcacc cgacccggcc
     1621 cgtttcggtg ccatgcgcgt cgggatgccg tacgtcgagt tcgacattcc agccggcacg
     1681 gatctggccg ccatcgcgaa gttctacaag gaaatcctgg gcggcatctc gggcgtgtcc
     1741 agcgacgaac gcgggcccta tgcgtgggcg aacacctcgg ccgaaagcag gttgatctac
     1801 cgcgagacaa ccgcgccggt tccggaatac gacgcgcacc acatccagat cacgctcgcc
     1861 gacttctccg gcccgcacaa gaaactactg gagcgcggtt tgatcaccga ggagagcgac
     1921 cagcaccagt accgcttcct ggacatcgtc gatgtcgata cgaacaagcc gctgttccg
//
LOCUS       DASWQN010000523         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_50446, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000523 DASWQN010000000
VERSION     DASWQN010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50446"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VGC82_08725"
     CDS             complement(<1..1152)
                     /locus_tag="VGC82_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0423391.1"
                     /translation="MEQRPLWQVRRGWWFGPGRGKTVPEGIAAGSGGAFPSGGGEVGT
                     LIRQFDWTSTSLGPLASWPQSLKTITDTLLRSPVPMVLLWGDDGVMIYNDAYSVFAGN
                     RHPKLLGSKVRDGWPEVADFNDHVMKVGLGGGTLAYRDQELVLHRHGGPEQVWMNLDY
                     SPVLNEDGQPAGVIAIVVETTQRVIAERTLKERENDLARVQRIAGIGGVTVDLGSGFN
                     NGRRSPEYLEIHGISADAVDTHEDWIKRIHPDDRDRTLRHFLAAVNGRDVRYSTEYRI
                     IRPNDGQVRWIACEAQIERDENGKPLRLVGAHMDITERTRAKELLRESEERFRLIANS
                     APVPMWVSKLDRTRSFANQAYLDFLGLDYEKAVTFDWRQILHPDDQSRVV"
     gene            complement(1152..>1504)
                     /locus_tag="VGC82_08730"
     CDS             complement(1152..>1504)
                     /locus_tag="VGC82_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY0423392.1"
                     /translation="LDAEMGPVVLFGSGGVDIELMKDVALAGAPLDAAEARQLIGRTK
                     AGVKMRGYRGKPAMHEASAVKALVGLSNLMADAGTRIASIDVNPFLVNARTGVAVDAL
                     IVLNNAAAKNAAPH"
BASE COUNT          264 a          489 c          445 g          306 t
ORIGIN      
        1 caccacccgc gattgatcgt ccgggtgcag gatctgacgc caatcgaaag tgaccgcctt
       61 ctcatagtcg aggccgagaa aatccaggta ggcctgattg gcgaagctgc gcgtgcggtc
      121 cagtttgctg acccacatcg gtaccggcgc gctgttggcg atcagtcgga aacgctcttc
      181 gctctcccta agcagttcct tggcccgcgt acgttcggtg atgtccatgt gggcgccaac
      241 gaggcgcagc ggtttgccgt tttcgtccct ttcaatctgc gcttcgcagg cgatccatcg
      301 cacctgtcca tcgttgggcc ggatgatgcg gtattccgtg gaatatcgaa cgtcccttcc
      361 attcaccgcg gcgaggaagt gccgcagcgt ccggtcgcga tcgtcaggat gaatgcgctt
      421 gatccagtct tcatgggtat cgaccgcatc agcgcttatc ccgtggatct ccagatattc
      481 gggtgagcgg cgtccgttgt tgaagccgct cccgaggtcg accgtgaccc ctccgatccc
      541 ggcgatcctc tggacccggg cgaggtcgtt ttcgcgctcc ttgagcgtcc gctcggcgat
      601 cacgcgctgg gtggtttcga ccacaatagc gatcacgccg gccggctggc cgtcctcatt
      661 cagaacaggg gaatagtcca ggttcatcca gacctgctcc ggaccgccat ggcggtgcag
      721 gaccagttcc tgatccctgt aggccagggt gccgccgccg agtccgacct tcatgacatg
      781 gtcgttaaag tctgcgacct cgggccaacc atcgcgcact ttcgacccga gcagctttgg
      841 gtgcctgttg ccggcaaaga cggaataggc gtcgttgtag atcatgacgc catcgtcccc
      901 ccacagcagc accattggga cgggggaacg cagcagggta tcggtgatgg tcttcagact
      961 ttgcggccag gaggccagcg gcccaagact ggtgctagtc caatcgaatt gccgtatcag
     1021 cgtgccgact tcgccaccac cgctcggaaa cgctcctcct gagccggcag ctattccttc
     1081 gggcaccgtt ttccccctgc caggtccgaa ccaccagcct cgacgaacct gccacaaggg
     1141 tctttgttcc atcaatgggg ggcggcgttt ttggctgccg cgttgttgag aacgatcagg
     1201 gcatcgacgg caacgccggt cctggcattg accaggaacg gattgacgtc aatcgaggcg
     1261 atccgggtac cggcgtcggc catcaaattg gacaggccga ccagcgcctt taccgccgac
     1321 gcctcgtgca tcgccggttt gccgcgataa cccctcattt tcaccccggc cttggtccgg
     1381 ccgatcaact gcctggcttc ggccgcgtcg agcggagcgc cggcaagcgc gacatccttc
     1441 atcagctcga tatcaacgcc gccggaaccg aacagcacga ccgggcccat ttcggcgtcg
     1501 agcg
//
LOCUS       DASWQN010000524         8850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2425013, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000524 DASWQN010000000
VERSION     DASWQN010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2425013"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VGC82_08735"
     CDS             <1..459
                     /locus_tag="VGC82_08735"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0423393.1"
                     /translation="NVDDEAVAKILNYPNAIIGLSDGGAHVQFQSGFGFSTRLLGEWV
                     REKKIMSLETAVRRLTFESAMVFGLHDRGLLLPGMVADIVVFNPDTVRPLPHEVVHDF
                     PTGAMRIREPAEGILMTVVNGQILLEDGKHSGALPGRVLRNPYYRGNHPG"
     gene            582..977
                     /locus_tag="VGC82_08740"
     CDS             582..977
                     /locus_tag="VGC82_08740"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY0423394.1"
                     /translation="MALTLEEANRIVEGAVAKAKDLNIRISVAVCDAGGRLVAFQRMN
                     NAIWASVYGSQGKAVASAAFGRPSGELSERANHPTPAGIAAAEGGHMIMGQGAVPILR
                     NGVVEGACGVGGGTAQQDEDCARSGASKV"
     gene            1126..2061
                     /locus_tag="VGC82_08745"
     CDS             1126..2061
                     /locus_tag="VGC82_08745"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0423395.1"
                     /translation="MSDAARAQPSKRIEQLAPELGRIISVSEPIENLADGFGGEQGPA
                     EGPLWWKEGHYLLFSDIHNNKRMKYVPGTGVSVFQEPTNRANGLTRDLQGRLLACEHD
                     TRRVTRQELDGSLTVIANSFQGRQLNRPNDVVVKSDGCIYFTDPWTSPAAPQQWDLTF
                     SGVYRITPDLGTMTLLIGDFVIPNGLAFSPDEKVLYINDSRRGHIRAFDLMPNGTLAK
                     QSDRIFVDLRGSEPGVPDGMKVDTEGNVYCGGAGGLWVMDPNGKKLGRIVHGASATTN
                     LAFGGDDWKTLYFTSRNHLGSVKVLIPGMPVPARR"
     gene            complement(2255..3379)
                     /locus_tag="VGC82_08750"
     CDS             complement(2255..3379)
                     /locus_tag="VGC82_08750"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0423396.1"
                     /translation="MSRIYNVIDSDGHILEPLSLWIDYMDPAFRDRAPRLERDANGRE
                     RLLIGEKMLGGERGMASLGAVGSRDGTVVVDDASEYKDGRKGGFDPHARIPDMDLDGI
                     DAAYLYPSIGLFAGSVEDPKLSAAICRAYNRWLADYCKPYPDRLFGIAMLPLQHVDLA
                     IEEMRFARHELGMPGIFLRPNPYNNKMVHDPYYERFWSAVEDCDVAVGFHEGGSSGMP
                     QVGLDRFETRGARHIVTHTMEMMMACLSMVWGGVCDRHPKLRVAFLESGGGWIVPWLE
                     RMDRHYVDQGFNDSGLSMKPSEIFQRNCWISFEPVEGSLRVLADYIGPHKILWATDYP
                     HPDGFFPGAPDMVRKNLEGLSAETRQQVMAGGAKGFYNLH"
     gene            3755..4600
                     /locus_tag="VGC82_08755"
     CDS             3755..4600
                     /locus_tag="VGC82_08755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY0423397.1"
                     /translation="MQFGASIFFTDYSISPAELAVALEERGFDSLWAAEHSHIPVPRR
                     TPPPGGGELPKRYYDVMDPFVTLTAAAAATKTLKVGTGVCLVIQRDTIQTAKLVASID
                     QVSGGRFLFGIGGGWNVEEIEAHGTVFGTRMKKMREQIEAMKVIWTKSTAEYNGEIVK
                     VPSMMTWPKPVQKPHPPVILGGAYPWAARRAIRYGDGWYPNASSGNPDDYLPGFRKMA
                     AEAGRDAASLPVTLGGVPEDLGKLRHFRDLGVARMNVTLPPEGKEQILPILDRWAGLV
                     RQLGT"
     gene            complement(4672..5796)
                     /locus_tag="VGC82_08760"
     CDS             complement(4672..5796)
                     /locus_tag="VGC82_08760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0423398.1"
                     /translation="MRIGLFYQIQVPKPWNPASESQRIYEALEQIPYAEQQGFASVWF
                     SEHHFRPEWSHNSAPDLTLAAVSQRTSRIRLGIGVVLAPIHHPLHVAARMATLDILSH
                     GRVDVGLGRTGYPYQLTPFGSDLADTRGMWQEFADVLPRIWTQETVSYDGKYFQIPPR
                     EVLPKPLQRPHPPLWSACGSDETARLTGALGMGGLFGSEGGPDRVRELMAIYQDALWT
                     ARDDATRTGRRTALMTAGYCHEDPLVVAERGTELVAWYIEQQRERARLVWRDYDPATV
                     PADYQSYYARDQRLAAGPHPGEPTPQAVREQGTSFCVGTPGDCIRFLELYESLGIEEV
                     FLLCAIGPAHHQEVMNTIRLFGEQIIPHFQAKKTDTPSLS"
     gene            5992..7044
                     /locus_tag="VGC82_08765"
     CDS             5992..7044
                     /locus_tag="VGC82_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HEY0423399.1"
                     /translation="MARLYANILETVGHTPVVKIGKLAPPGVSLYVKVEAFNPLGSVK
                     DRLALGVIEAAERSGALKPGQTVIEATSGNTGIGLAMVCAQKGYPLVIVMAEPFSVER
                     RRLMRFLGAKVVITPAAERGMGMINKTLELAAANGWFLTRQFENEANADMHTRTTARE
                     IIDDFQGEKLDYWVTGYGTGGTLKGVSRVLAKERPETKIIVCEPDDAALLSSGTPQHR
                     RTDGSPAEGHPAFNPHPMQGWTPDFIPKLTGDAVESGAIHKVLRIKGADALRCSRELA
                     QKEGIFVGITSGATFAGALAVCAEAPEGSTILCMLPDTGERYLSTPLFADISADMSEE
                     ELEISRSTPNNQLKPL"
     gene            7293..8543
                     /locus_tag="VGC82_08770"
     CDS             7293..8543
                     /locus_tag="VGC82_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0423400.1"
                     /translation="MLSRRLGGVLLAVILLAGSFAAKAQVPITIGFGMALTGGLAPIG
                     KAALFAMQIWEKETNAHGGVLGRPVKLVFYDDQSNPSLIPGIYTKLLDVDKVDIVVSG
                     YATNMIAPAMPVVIGHNRTFLTLFGLATNSEFNYPKYFAIQPVGGPKPKEAFARGFFS
                     VAMAQNPKPTTVAMVGADAEFPRNAMDGARIVAKEMGLKTVYDKTYPPTTSDYSPIVR
                     AIQATNPDIILVCSYPPDTVGIIRAANEIGLKAKMFGGGMVGLQSTAIKVQLGPLMNG
                     IVDYDFWLPWSKLATPASLEFLKKYQDGASAQGIDALGYYLPPFAYAYMQVLQQAIET
                     SKSLNDDKLADALRKDTFKTIVGDIKFGSNGEWIEPRVLAVQFQNVKANDLEQFRDSK
                     TEVILWPPELKTGELQYPYSEVKR"
BASE COUNT         1736 a         2735 c         2762 g         1617 t
ORIGIN      
        1 aatgtcgatg acgaggccgt tgccaaaatc ctgaactatc cgaatgccat catcggattg
       61 tcggacggtg gcgcgcatgt gcagttccag agtggcttcg gcttcagcac acgtctgctg
      121 ggcgagtggg tgcgcgagaa aaaaatcatg tcgctggaga ccgcggtgcg gcggctgacg
      181 ttcgaatcgg ccatggtctt cgggctgcac gatcgcggcc tgctgttgcc cggcatggtc
      241 gcggatatcg tggtgttcaa tcccgatacc gtccgcccgc taccgcacga ggtggtgcat
      301 gacttcccga ccggggccat gcggatccgt gagccggcgg aaggcatcct gatgaccgtc
      361 gtcaacggcc agatcctgct ggaagacggc aagcatagcg gcgcgcttcc cggccgggtc
      421 ctgcgcaatc cctattatcg gggcaaccat ccggggtagc ccgcggcgcc gggcgtcggg
      481 caatccacgt ccggcatcgt aaggggagaa acgtcccgcg tgcggacgtg cctgggacgt
      541 gcggcgcgtg aactcaaagc ctcagaagaa ggaagtcaac gatggccctg acgctggaag
      601 aagccaaccg catcgtcgaa ggcgctgtgg cgaaagcaaa agatctcaac atccggatta
      661 gcgtcgcggt ctgtgacgcc ggcggccggt tggtcgcgtt tcaacgaatg aacaacgcaa
      721 tttgggcgag cgtctatggc agccaaggca aggccgtcgc atcggctgcg ttcggccgtc
      781 caagcggcga gctatccgaa cgggcaaacc atccaacgcc ggccggtatc gccgcggccg
      841 agggtggcca catgatcatg ggccaaggcg ccgtgccgat cctgcgcaat ggcgtcgtcg
      901 agggagcctg cggcgtcggc ggcggcaccg cccaacaaga cgaagactgc gctcggtctg
      961 gagcgagcaa agtataactg tcaataacac ttgggaaggt gcaaatgaac caagatgata
     1021 ttcgccggat ggccgctccc gttggaggag gaaaccatga gcgttcagac cggcatttca
     1081 cgccggcaca cattggcgat ggctgtggcg ataggaggcc ttacgatgtc tgacgccgct
     1141 cgcgcccaac cgtcgaagcg aatcgaacaa ttggcaccgg agctgggaag gatcatttcg
     1201 gtgtcggagc ccattgagaa cctggccgat ggcttcggcg gagaacaggg cccggcggaa
     1261 ggaccgctct ggtggaagga aggccactat ctgttgttca gcgacattca caacaacaag
     1321 cgcatgaaat acgtgcccgg gaccggtgtg agcgtgttcc aggagccgac gaaccgcgcg
     1381 aatgggctga ccagagacct tcagggccgg ttgctcgcct gtgaacacga cacgcgccgg
     1441 gttacccggc aggaacttga cggcagcctg acggtcatcg cgaacagctt ccagggacgg
     1501 cagctcaacc ggcccaacga tgtcgtggtg aagtccgatg gctgcatcta ctttaccgac
     1561 ccgtggacca gccccgccgc tccgcagcag tgggacctga ccttctcggg cgtgtatcgc
     1621 atcacccccg acctgggcac gatgacgctt cttatcgggg attttgtcat ccccaatggt
     1681 ctcgcgtttt cgcctgacga gaaggtgctt tacatcaacg actctcgccg cggccacatc
     1741 agggccttcg acctgatgcc caacggcacg cttgccaagc agtccgaccg gatcttcgtg
     1801 gacctgcgcg gcagcgaacc gggcgttccc gacggcatga aggttgatac cgaaggcaac
     1861 gtctattgcg gcggcgccgg cggcttgtgg gtcatggatc cgaacggaaa gaagctgggc
     1921 cgcatcgtgc acggtgcttc cgccaccacc aacctcgcgt tcggcggcga tgactggaag
     1981 acactttact tcaccagtcg caatcacctc ggatcagtga aagtattgat ccccggtatg
     2041 ccggtgccgg cacggcgctg actaggtttc cgtccgcaat aactgcgccg gtcgcgcgtg
     2101 tcccggtcga gcgtgtcccg gtagatccgt catggtgggc aaaggcctac tctgacggga
     2161 tgctcctacc gtgcagcaat cggagcagtt aatcgtgagt ggaccctgaa tagccaatgg
     2221 cgtggcacgc cggcacgcgc cacgccatac ttgttcagtg caggttgtag aagcccttgg
     2281 cgccgccggc catcacctgc tgcctggttt ccgccgaaag cccttcgagg ttcttccgca
     2341 ccatgtcggg cgcaccgggg aagaagccgt cgggatgcgg atagtcggtc gcccacagga
     2401 tcttgtgcgg cccgatgtaa tcggccagca ctctcaggct gccttccacc ggctcgaacg
     2461 aaatccagca attgcgctgg aagatttcgc tcggtttcat cgacaggcca gagtcgttga
     2521 agccctgatc gacatagtga cggtccatcc gctccagcca cggcacgatc cagccgccac
     2581 cggattccag gaacgccacg cggagcttcg gatgccggtc gcatacgccg ccccagacca
     2641 tgctgaggca cgccatcatc atttccatcg tgtgcgtcac gatgtgacgg gcgccgcgcg
     2701 tctcgaagcg gtccaggccg acctgcggca tgccgctgct gccgccctca tggaatccca
     2761 ccgcgacgtc gcaatcctcg accgccgacc agaaccgctc gtagtacggg tcgtggacca
     2821 tcttgttgtt gtagggattt ggccgcagga agatgccggg catacccaat tcatggcggg
     2881 caaagcgcat ttcctcgatc gccaggtcaa cgtgctgcaa cggcagcatc gcaataccaa
     2941 acaggcggtc tggatagggt ttgcagtagt cggcgagcca gcgattgtag gcgcggcaga
     3001 tcgccgccga cagcttcgga tcctcgacgg agccagcgaa caagccgatg ctcggataga
     3061 ggtacgcggc atcgatgccg tcgagatcca tgtcgggaat gcgggcgtgc ggatcgaagc
     3121 cgcccttgcg tccatcttta tattcgcttg catcgtccac tacgacggtg ccgtcgcgtg
     3181 acccgactgc gccgagcgac gccatgccgc gttcaccgcc aagcatcttt tcgccgatca
     3241 gcagtcgttc ccgcccgttg gcgtcgcgct ccagcctcgg cgcgcggtcc cggaaggccg
     3301 ggtccatgta gtcgatccag agactgagcg gctcaagtat gtgaccatcg gagtcgatga
     3361 cgttgtaaat gcggctcatg accattcctc ctgttgacgt ttttcttttc gcaccgcttg
     3421 gcggccgatg gtgaacgttt gtctggtccg aagcggtgac ccagacagcc ggccgcggtg
     3481 cgggccgaca cgttgggttc caccattgca ctcgccctcg gcgggagcaa tggtggttgg
     3541 tctgtccgcg gatcttccag gctacccatc ggcattggct ggagcggttg aacctggttc
     3601 gttgacttca accgctcggt ctgttgatga tgacgcgctt catggtcttg cgcgacgact
     3661 ggacacagga agcagggcaa gcgtcgccaa ggcagcggtg ccttgacggc ggccgccggc
     3721 cgagggaaac gctgtaccaa cgaaagtaat aatcatgcaa ttcggtgctt caatcttctt
     3781 caccgactac tcgatctccc cggcggaact cgccgtcgcg ctggaggagc gtggttttga
     3841 ctcgctttgg gcggccgaac attcccacat cccggtgccg cggcgtaccc cgcccccggg
     3901 cggcggcgag ctgcccaagc gctactacga cgtgatggac ccattcgtca ccctgaccgc
     3961 cgccgcggcg gccaccaaga cgctcaaggt cggcaccggg gtctgcctgg tgatccagcg
     4021 cgacacgatc caaacggcaa agcttgtggc ctcgatcgat caggtcagcg gcgggcggtt
     4081 tttgttcggc atcggcggcg gctggaatgt cgaggagatc gaggctcacg gcaccgtatt
     4141 cggcacccgc atgaagaaga tgcgcgagca gatcgaggcg atgaaggtga tctggaccaa
     4201 gtcgacagcg gagtacaacg gcgagatcgt caaggttcca tcgatgatga cgtggcctaa
     4261 accggtgcag aagccgcatc cgccggtcat tcttggcggc gcctacccgt gggccgcgag
     4321 gcgcgcgatc cgctacggcg atggctggta tccgaacgcc agcagcggca acccggacga
     4381 ttacctgccc ggcttccgca agatggccgc cgaagccgga cgtgatgccg catcgctgcc
     4441 ggtgacgctt ggcggcgtcc cggaagatct tggcaagttg cggcactttc gcgacctcgg
     4501 cgtcgcccgg atgaacgtga ctctgccacc cgagggtaaa gaacagatcc tgcctatact
     4561 cgatcgctgg gcggggctgg tgcggcaact gggcacctga caggctgaac cgccgaggcg
     4621 gctggcaagg ctgcctcggt cccgccccag cctacgacta aagacttcgg cctacgacaa
     4681 tgacggcgtg tctgtcttct tcgcctggaa gtgcgggatg atctgctcgc caaatagtcg
     4741 aatcgtgttc atcacttcct gatggtgcgc cggaccgatg gcgcacagca agaagacctc
     4801 ctcgataccg agggattcgt acaactcaag aaaccggatg cagtcccccg gtgtgccgac
     4861 gcagaacgac gtgccctgtt cgcgcacggc ctgcggtgtc ggttcccctg ggtgcggccc
     4921 ggcggcgaga cgctgatcgc gcgcatagta gctttggtaa tccgccggca cggtggcggg
     4981 gtcgtagtcg cgccacacca gccgcgcacg ttcgcgctgc tgttcaatat accaggccac
     5041 gagttcggtg ccgcgttccg ccaccacaag tggatcttcg tggcagtatc ccgcggtcat
     5101 cagcgcggtg cgccgaccgg ttctcgttgc gtcgtcccga gccgtccaca gtgcatcctg
     5161 gtagatggcc atgagttcgc ggacgcgatc cggtccgcct tcgctgccga acaatcctcc
     5221 catacccaga gcgcccgtca ggcgcgcggt ctcgtcgctg ccgcaggcgg accacagtgg
     5281 aggatgcggc ctttgcaaag gctttggaag gacctcccgc ggcgggatct gaaagtactt
     5341 cccatcatac gacacggtct cctgcgtcca gatgcgaggc agcacgtcgg cgaattcttg
     5401 ccacatgccg cgcgtgtccg ccaaatccga gccaaacggc gtgagctgat agggataccc
     5461 ggtgcggccc aggcccacat ccacgcgccc atggctcaaa atgtcgagag tcgccatccg
     5521 cgctgcgacg tggagcggat ggtgtatcgg cgccaggacc acgccgatgc caaggcggat
     5581 gcggctggtg cgctgactga ccgccgcgag cgtcaggtcc ggcgcgctgt tgtgcgacca
     5641 ctcaggacga aaatggtgtt cggaaaacca tacactcgcg aatccctgct gctcggcata
     5701 aggaatttgt tccagggctt cataaatgcg ctgagattca cttgcaggat tccagggttt
     5761 gggaacctga atctgataaa acaggccaat cctcatgtga tcgtctcctg tacaatgaac
     5821 gacgcttact gcctgacggc atgacgggcg ggcgccttgg cacttacaac tttattaatc
     5881 tgctttaccg gctattttct tcctgcgtaa gttccacccc agtgaaaaca cgaaactcgg
     5941 caacagcggt gccccaagct ccgcaacagg cggagtcggg ggagcaacat catggcacgc
     6001 ttatacgcaa atatactcga aacagtcggc cacacacccg tcgtgaagat cggcaagctg
     6061 gccccgccgg gcgtcagtct ttacgtcaag gtcgaggcat tcaacccgtt gggttcggtc
     6121 aaggatcgtc tggcccttgg cgtgatcgag gcggcggagc gcagcggcgc gcttaagccg
     6181 ggccagacag tcattgaggc gaccagcggc aacaccggta tcggcctcgc gatggtctgc
     6241 gcccagaagg gctacccact ggtgatcgtc atggccgaac ccttcagcgt cgagcgccgc
     6301 cggttgatgc gcttccttgg cgccaaggtc gtgatcaccc cagcggccga acgcggcatg
     6361 gggatgatca ataaaacgct tgagcttgcg gcggcaaatg gctggttcct tacgcggcag
     6421 ttcgaaaatg aagccaacgc cgatatgcat acccgtacca ccgcccggga gatcattgat
     6481 gacttccagg gcgagaagct cgactactgg gttaccggct atggcacggg cggcacgctg
     6541 aagggcgtct cccgcgtgct ggccaaggag cggccggaaa ccaagatcat cgtgtgcgaa
     6601 ccggatgacg cagcgctgct ttccagcggc acgccgcagc accggcggac cgatggctcc
     6661 ccagccgagg gtcacccggc cttcaatccc catccgatgc aagggtggac gccggacttc
     6721 atcccgaagc tgaccggcga cgcggtcgag tcgggcgcca tccacaaggt attgcggatc
     6781 aagggggccg acgctttgcg ctgcagccgg gagcttgcgc agaaggaggg tatctttgtc
     6841 ggcatcacct cgggtgccac ctttgcgggc gctcttgcgg tttgtgccga ggcgccggaa
     6901 ggctccacga tcctgtgcat gttgccggac accggcgaac gctatctcag cacaccgctg
     6961 ttcgcggata tatccgccga catgtcggag gaagagctgg aaatctccag gtcaaccccc
     7021 aacaaccagc tcaagccgct ttgactttcc ctggaagcgc aaagcaaacg ccgcgctgcg
     7081 gccacgccgt ttcgctccac cagccttgtc gcatgttcca ggaggccggc aaacagcgcg
     7141 gcggagcgcc accgctccaa tagggaacca ttccggacct cgggttgcgg atcgaccgtg
     7201 aaggtgctcg acttctcggc aaagcggcga cgcgcgcggt ttacactggg atatctgctg
     7261 aacgccgccg acgaacaagg ggaggaatat caatgctatc caggcgtctg ggcggcgtgc
     7321 tgctcgccgt tattctgctg gccggaagtt ttgcggctaa agcgcaagtt cccatcacga
     7381 tcggctttgg catggcattg accggcggcc tcgcgccgat cggcaaggcg gcgctgttcg
     7441 ccatgcagat ctgggaaaag gaaaccaacg cccatggcgg cgtcctgggc cggccggtga
     7501 aactggtgtt ctacgatgac cagtcgaacc cttcgctcat tcccggcatc tacacgaagc
     7561 tgcttgacgt tgacaaagtc gatatcgtgg tcagcggata cgcgaccaac atgatcgcgc
     7621 cggcgatgcc ggttgtgatc gggcacaacc ggaccttcct tacactgttt ggcctcgcaa
     7681 ccaactctga attcaactac ccgaaatact tcgcgataca acccgtcggc ggcccgaagc
     7741 caaaggaggc cttcgcacgc ggcttcttca gcgtggcgat ggcgcagaac ccgaaaccca
     7801 cgaccgtcgc catggtcggt gccgatgcgg agttcccgcg caatgccatg gatggcgcgc
     7861 gcatcgtggc gaaggagatg ggtctgaaga ccgtctatga caaaacctat ccgccgacca
     7921 cgagcgatta ctcgcccatc gtacgcgcca tccaggcgac caatcccgac atcatcctgg
     7981 tttgctccta tccgcccgat acggttggca tcatccgagc ggccaatgag atcggactga
     8041 aagcgaagat gttcggcggc gggatggtcg ggctgcagag cacggcaatc aaagtgcagt
     8101 tgggcccgct gatgaacggg atcgtcgatt atgacttctg gctgccgtgg tccaagctcg
     8161 cgaccccggc cagtcttgaa tttctgaaga aataccagga cggggcatcg gcccaaggga
     8221 tcgacgcgct gggctattac ctgccgccat tcgcctatgc ctacatgcaa gtgctgcagc
     8281 aggcgatcga gacctcgaaa agtctgaacg acgacaaact ggcggacgca ctgcgcaagg
     8341 ataccttcaa gacgatcgtg ggcgatatca agttcggctc caacggcgag tggatcgagc
     8401 cgcgcgtact ggccgtccag ttccagaacg tcaaggcgaa tgacctcgaa cagttccgcg
     8461 attccaagac cgaggtgatc ctctggccgc cggagctgaa gacaggtgaa ctgcaatatc
     8521 catattcgga ggtaaagcgt tgatcagtgc ttgataggaa ggccgaggcg ggacggtagc
     8581 gagtttccgc cggggcctta ctctagcggg ttgcgtcgat cggccgacgg cttgttaggg
     8641 tgccgcactc ctgatgcccg ccccggcccg gagcattctg caaaccttga tcatccgttc
     8701 ccaggggagg gtctcgccgt tgcgccaggc gctaggccgc ggcctcagag cggttgttct
     8761 aagccctgga agccggccgc cgacgtctgc ggacgtttgc gaggccgcca agtccagcag
     8821 ccagtacaag gatcgaagtc ggttccggca
//
LOCUS       DASWQN010000525         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1898112, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000525 DASWQN010000000
VERSION     DASWQN010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898112"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..333)
                     /locus_tag="VGC82_08775"
     CDS             complement(178..333)
                     /locus_tag="VGC82_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423401.1"
                     /translation="MFLKFGFHVSGSNISILGALIYGTLNWHYRDRRRDRVTDQIVRD
                     RYEHNQT"
     gene            374..589
                     /locus_tag="VGC82_08780"
     CDS             374..589
                     /locus_tag="VGC82_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423402.1"
                     /translation="MGLASYRIFGVPGAWHVDHEGKAAHTYQTKEAAFEAAVAAASLA
                     LRQGHEVSVTAPGSGDREATTGARDVK"
     gene            586..834
                     /locus_tag="VGC82_08785"
     CDS             586..834
                     /locus_tag="VGC82_08785"
                     /inference="COORDINATES: protein motif:HMM:NF015069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HEY0423403.1"
                     /translation="MIQALGVMAAMLTSLSYIPQVRKAWPRGSTDDLSFRTLGILAAG
                     LSLWIAYGLAKSDAVIIVANAVGLFLILLLIGLKWRDA"
     gene            847..1485
                     /locus_tag="VGC82_08790"
     CDS             847..1485
                     /locus_tag="VGC82_08790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY0423404.1"
                     /translation="MTFDNPKTASDGAALVPIEDAVHLCVDMQRVFSKGGLWQTPWME
                     RVLPAIATVAALCPARTVFSRFITPARADEAPGQWQRYYRRWAAATREKLGPSQLDLV
                     PELARYAPPATIVDKPAYSAFHRSSLANFLAERHVRTLIVSGAETDVCVLSTVLDAVN
                     LGYRVVILQDCLCSSSDEGHDALMTLYRLRYSEQIDLATADMLPEIWADLAA"
BASE COUNT          277 a          473 c          476 g          304 t
ORIGIN      
        1 gccgagcggt tcaagaaagc tgcggagcag gcgcccgccg gcatggcccg cgagttgtta
       61 ctcaggcgag cccggcaggc agagacagct tcccgtgtga acgactggat ccgatcgcca
      121 ggcttgcagc cgccgaaata atatcgcgcg cccgatggcg cctgctcagg ctgccaatca
      181 ggtctggttg tgctcgtatc tgtcgcggac gatctggtcc gtcacgcggt cacgacgcct
      241 gtctcggtag tgccagttga gcgtcccgta aatcagggcg cccaagattg agatgttcga
      301 gccggaaacg tggaagccga acttcaggaa cattgccgcg tcagcatcgt ttgcctgtca
      361 ggaggatctt tccatgggac tggcgagcta caggattttt ggcgtgccgg gcgcttggca
      421 cgtcgaccac gaaggcaagg cagcgcacac ctaccaaacc aaggaagccg cgttcgaggc
      481 cgccgttgcc gcagcgtcgt tggcattgcg acaggggcat gaagtcagcg tcaccgcgcc
      541 agggagcggc gatagagagg ccacgaccgg tgcccgggac gtcaagtgat ccaggcattg
      601 ggggtcatgg cggcgatgct cacgtcgcta tcgtatattc cccaggttcg caaagcatgg
      661 cctcgcggtt cgacagacga tctgtcgttc cgaacgcttg ggatcctggc ggcaggcctg
      721 tcgctctgga ttgcctacgg actggcgaaa agcgatgccg tgatcatcgt ggccaatgcg
      781 gtgggacttt ttctcattct cttgctgata ggcctgaagt ggcgtgacgc ctgagagggt
      841 gcccgcatga cattcgacaa tcccaagacc gccagcgacg gagcggcgct tgttccgatc
      901 gaggatgctg tccacctctg cgtcgacatg cagcgggtgt tctccaaagg cggactttgg
      961 caaactccct ggatggagcg ggtgttgccg gccattgcca ccgttgcggc cttgtgcccg
     1021 gctcggaccg tgttctcacg tttcataacg ccggctcgcg ccgatgaggc gcccggtcaa
     1081 tggcaacgct attaccgccg ttgggccgct gccacgcgcg agaagctcgg accgtcgcag
     1141 ctcgatctgg tacccgaact ggcccgctac gcgccgcccg cgaccattgt cgacaagccg
     1201 gcctactccg cgtttcatcg ctcgtctctc gcgaatttcc ttgccgagcg gcatgtcagg
     1261 accctcatcg tatccggcgc cgaaaccgat gtctgcgttc tgtcgacggt gctggatgca
     1321 gtgaacctcg gttaccgcgt cgtcattctg caggactgcc tgtgcagctc ctcggacgag
     1381 ggtcatgacg cgctcatgac cctgtaccgg cttcgctata gcgagcagat cgacctcgct
     1441 acggccgaca tgcttcccga gatttgggct gacctcgccg cataggaacg aatgcgttca
     1501 acaattgttg aaatcccccg acagccaccg
//
LOCUS       DASWQN010000526         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2161618, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000526 DASWQN010000000
VERSION     DASWQN010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161618"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VGC82_08795"
     CDS             <1..596
                     /locus_tag="VGC82_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY0423405.1"
                     /translation="RSRWIKALLVLYMLPWALPALPAYLSLHWMLIGYGGFLNSALEA
                     LFDIDGPIWFNSYWLALGANILAYIWKWMPFWTLVFLAGRMAIPQDIYEAAAVDGATG
                     IRRFTFVTFPLLANLYLICTLLSTLWTVGDFTAAYFVSSGAPALTTEVLATYAFRMAF
                     EHGYPELAIAAVMSALPVLIPITILLMRRLQATELQI"
     gene            605..1480
                     /locus_tag="VGC82_08800"
     CDS             605..1480
                     /locus_tag="VGC82_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0423406.1"
                     /translation="MTTGQRKRRVRFRPRKIVANAGAAVLGMAIAIWTVVPVYNMVLI
                     ALSDDGDEFSGTVWPDDPSLESFRVIWNESYWYLENFWHQFGNSLYIGLATMAATVVI
                     GSFASFAMGRMRLRKAWLIGNFSLITYAVPSSFLVIPFTHLMHIYGLTDNLWSVIASQ
                     VAFATPYAILILHQYGKLIPLELDEAARVDGAGPFQVYWRIYLPLMAPALAAVATYAL
                     LVAWNEYLYQFLLLSSPRSMTVAVAIDQFFDSDEAPWNYMMAVALVYSIPPIAVYYAL
                     RRYMVAGLTMGGVKG"
     gene            1536..>2102
                     /locus_tag="VGC82_08805"
     CDS             1536..>2102
                     /locus_tag="VGC82_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0423407.1"
                     /translation="MAIQLVSRAKSVREQVSPEEWAARVELAAGHRVLAHYGVNDMTY
                     NHFGLRVPGKPDQMLIKRTDWMFGEVTASSLLKVDFDGNVLTDTDVKTIKGGALIIHA
                     GLLKGRPDLNATLHTHTVSGMGVAAHKFGLLPINQHALRFHGELKYHAFEGLEFDHDM
                     TPKLLRDLDGGLFMILRNHGVLVCGGSVA"
BASE COUNT          365 a          631 c          642 g          464 t
ORIGIN      
        1 agcggagccg ctggatcaag gccctgctgg tcctctacat gctgccgtgg gccttgccgg
       61 cgctcccagc ctatctgtcc ctgcactgga tgctgatcgg ctacggcggc tttctcaaca
      121 gcgcgctgga ggcattgttc gatatcgacg gcccgatctg gttcaactcc tactggcttg
      181 ccttgggtgc gaacatcctt gcctacatct ggaagtggat gccgttctgg accctggttt
      241 tcctcgccgg ccggatggcg atcccgcagg acatctacga agccgccgcg gtcgatggcg
      301 ctaccggcat ccgccgcttc acgttcgtga cgtttccgct gttggccaat ttgtacctga
      361 tctgcacgct gctctccacg ctgtggactg tcggtgactt cacagcggcc tatttcgtct
      421 ccagcggcgc gcctgcgttg accacggaag ttctggccac gtacgccttt cgcatggcct
      481 tcgaacacgg ttatccggag cttgctatcg ctgcggtgat gtcggccctg ccggtgctga
      541 taccgatcac catcctgctg atgcgccggc tgcaggcgac ggaactgcag atatgagcct
      601 tgccatgacg acaggccaac ggaagcgccg cgtgcgcttc cggccgcgga agatcgttgc
      661 caacgccggc gcggcggtac ttggcatggc gatcgcaatc tggaccgtcg tgccggttta
      721 caacatggtg ctgatcgcgc ttagcgacga cggcgacgaa ttctccggaa ctgtctggcc
      781 ggacgacccg tcgcttgaaa gcttccgggt gatctggaac gagagctact ggtatctcga
      841 gaatttttgg catcagttcg gcaacagcct ttatatcggc ctggcgacaa tggccgcgac
      901 cgtggtgatc ggctccttcg ccagtttcgc aatgggacgc atgcggttgc gcaaggcgtg
      961 gctgatcgga aatttttcgc ttatcacata cgccgtccca tcctcgtttc tggtgatccc
     1021 gtttacgcac ctcatgcata tctacgggct gaccgacaat ctgtggtcgg tcatcgcgtc
     1081 gcaggtggcc ttcgccacgc cttatgcgat ccttattctg caccaatacg gcaagctgat
     1141 tccgcttgag ctcgacgagg cggcgcgggt cgatggcgcg ggaccgtttc aggtgtactg
     1201 gcgcatctat ctgccgttga tggcaccggc gcttgccgcg gtcgcgacct acgcgctgct
     1261 ggtggcctgg aacgagtacc tgtaccagtt cctgctgctc tcatcgccgc gcagcatgac
     1321 cgtcgcggtg gcgatcgacc agttcttcga cagcgatgag gcaccgtgga actacatgat
     1381 ggccgttgcg ctggtgtatt cgattccgcc gatcgcggtc tattacgctt tgcgccgcta
     1441 catggtcgcg ggcctgacga tgggcggcgt gaaaggttag gacgcgatca ggcctcgcgg
     1501 ggccggatat gcgccacggc aggggaggac gcaacatggc cattcaactt gtatccaggg
     1561 cgaaatccgt tcgcgaacag gtcagtcccg aggagtgggc agcccgtgtc gaactcgccg
     1621 cagggcaccg cgtgcttgcg cattacggcg tgaacgacat gacttacaat catttcggtc
     1681 ttcgggtgcc gggaaaaccg gaccagatgc tgatcaagcg tacggactgg atgtttggcg
     1741 aggtaacggc atcctcgctg ctgaaggtcg atttcgacgg caatgtcctg accgatacgg
     1801 acgtcaagac gatcaagggg ggcgccctga ttatccacgc cggcctgctg aaaggccgtc
     1861 cggatcttaa cgcgacgttg cacacccata cggtcagcgg catgggcgta gcggcgcata
     1921 aattcggctt gttgccgatc aaccagcatg cgcttcggtt ccatggagaa ttgaagtacc
     1981 acgcgttcga aggcctcgag ttcgaccacg acatgacgcc aaaactgctg cgtgacctcg
     2041 acggtggctt gttcatgatc ttgcgcaacc acggtgtcct tgtctgcggc ggaagcgttg
     2101 cc
//
LOCUS       DASWQN010000527         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1766009, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000527 DASWQN010000000
VERSION     DASWQN010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766009"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..687)
                     /locus_tag="VGC82_08810"
     CDS             complement(34..687)
                     /locus_tag="VGC82_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423408.1"
                     /translation="MPQLGRIGFWAGLAAATATIAYDVAQILQVLGVLHFPLDEILIF
                     GTSLCIVVPFVLEMLALHYATPIESRFWTHAGLIFTTMYAVFGTANYVIQLTTVIPAK
                     VRGMADTVRVLEQTPHSLLWDFDAMAYLAMGLTALLIIPALRSNGFEWRVRLACMAHV
                     VATVLAGIVYFYSTYSYRLLMLGFPWGITAPLFMLLVAIGLRRQHTERSLGSSEKTS"
     gene            complement(829..1923)
                     /locus_tag="VGC82_08815"
     CDS             complement(829..1923)
                     /locus_tag="VGC82_08815"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEY0423409.1"
                     /translation="MRIALAVFILLHGLIDLLGPAKAFAWADVSQLRAPISPAAGLLW
                     LAAAVLLIAAATGLLLRASWWWYSALPGVLLSQGLIMTAWGDAKFGTLANVIIAIPLL
                     VAALDTRPSSFRSRFEHDRNALLAGVTPAAPLVTESDLAALPPLMQAYLRRMGAVGRA
                     HVRNLRVTFQAQMRSSASAPWMQATATQYEFFDRPARLFHMNASRAGMPLDVFHRYVD
                     GAATFQVRIAGLIPMVDKRGTGLTNDETVTLMNDVLVMAAAAVLDLPFAFETIGERSL
                     RAIFHNAGFTVSAILTFDAAGDLVGFASTDRAHDREGGAATWSTPISGYRQVDGIRIG
                     ALGDANWIEPSGEWTYGRFEITSIAYNVTQ"
BASE COUNT          461 a          686 c          674 g          381 t
ORIGIN      
        1 ctgtcggagc ggttgctcca tggcccctcc tgctcaagac gtcttctcgc tgctgcccaa
       61 ggaacgctcg gtatgttgtc ttcgcagccc aatggctaca agcagcatga acaaaggtgc
      121 agtgatgccc cacgggaagc cgagcatcag cagtcggtaa gagtacgtcg agtaaaaata
      181 cacgatgccg gccagcaccg tcgccacaac atgagccatg caggctaacc gcacccgcca
      241 ctcaaaaccg ttactgcgga gggctggaat gatcagcaat gccgtgagcc ccatcgccag
      301 gtatgccatc gcatcgaagt cccaaaggag cgaatgcggc gtctgttcga gcacccggac
      361 cgtgtccgcc atgccccgaa ccttcgctgg aattaccgtg gtgagttgga tgacgtaatt
      421 tgcggtgccg aacaccgcgt acatcgttgt gaagatgagc cccgcatggg tccagaatcg
      481 actttcgatc ggcgtggcat agtgcagagc gagcatttcc agaacgaacg gaacgacgat
      541 gcaaagcgat gtaccgaaga tcagaatctc gtccagggga aaatgcagaa cgccgagtac
      601 ctgtaggatt tgcgccacgt cataggcgat ggtcgctgta gctgcagcta gccctgccca
      661 gaatccgatc cggccgagtt gaggcataga aggagatcct cgaaagccgc cgcatgccgt
      721 agccctaacg cccaagctaa gctgcagggg agttccaagt gcgagcgcag cgagcttcca
      781 aagatcgcct gacagcttca gcgttcgttt agccgaccgg cccgccgttc actgtgtcac
      841 gttgtaggcg attgacgtga tctcgaaacg gccatatgtc cactcgccgg agggctcaat
      901 ccagttggca tcgccgaggg cgcctatccg gattccgtca acttggcgat acccggaaat
      961 tggcgtggac catgtcgcgg cgccgccctc ccggtcgtgc gcccggtcgg tggacgcgaa
     1021 tccaacgaga tctccggcgg cgtcgaacgt caggatggcc gacaccgtga accccgcgtt
     1081 gtggaagatc gctcgcaacg acctttcgcc gatggtctcg aaggcgaacg gcaggtccag
     1141 gacagccgcc gccgccatga cgagcacgtc attcatgagc gtgactgttt cgtcgttcgt
     1201 cagccccgtg ccgcgcttgt ccaccatagg tatcagccca gcaatccgca cctggaacgt
     1261 ggcggcgccg tcgacgtacc gatggaagac gtccaatggc atgcccgccc ggctcgcgtt
     1321 catgtggaac aaccgggccg gccggtcgaa gaactcgtac tgcgtggcgg tggcctgcat
     1381 ccacggcgcc gacgcgctgc tgcgcatttg cgcctggaag gttacacgca ggttgcgcac
     1441 gtgcgcacgt ccaacggcgc ccatccgcct aaggtatgcc tgcatgaggg gcggcagtgc
     1501 cgcgagatcg ctctcggtca ccagcggcgc cgcaggcgtc actccggcca gcagcgcgtt
     1561 gcggtcatgt tcgaagcgtg aacggaaact agacgggcgc gtatccagag ccgcgacgag
     1621 cagcgggatc gcgatgatca cgttggcgag ggtgccgaac ttcgcgtcgc cccatgctgt
     1681 catgatcaac ccctgtgaca gcagtacccc gggcagtgcc gagtaccacc accacgaggc
     1741 ccgcaggaga agtcccgtag cggcggcgat cagcagtacc gccgcggcga gccacaagag
     1801 gccggccgcg ggagaaatcg gtgcccgcag ttggctgacg tccgcccatg cgaaggcctt
     1861 ggccgggccc agcagatcga tcagaccgtg cagcaagatg aaaactgcga gtgcgattcg
     1921 cattatgaat ttctccttga ggacgaggcg caggtgtccg atgcttcaca cgattaccgc
     1981 ctctactggt cgtcgcaggg accgcggcat ctcaggtcct cgaccaaacc ggaccactag
     2041 atctggtcgg tgaggactca caccaaggaa cgttgcgaac tgtggtcgca gggttgagac
     2101 ctcaaccggc tgattcagga acgcatttcg aatgcccaga gcggtcgcct gcaaggcgaa
     2161 gcgctcgtag cagcgtccgg cctcaaccca atgcgccttg tt
//
LOCUS       DASWQN010000528         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2029724, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000528 DASWQN010000000
VERSION     DASWQN010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029724"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..217)
                     /locus_tag="VGC82_08820"
                     /pseudo
     CDS             complement(98..217)
                     /locus_tag="VGC82_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            265..1549
                     /locus_tag="VGC82_08825"
                     /pseudo
     CDS             265..1549
                     /locus_tag="VGC82_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611960.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    552..668
                     /locus_tag="VGC82_08825"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          305 a          496 c          552 g          319 t
ORIGIN      
        1 ttgaggttct tgcaaaattc aaggatccgg tcgattttta ttgcgtgcag cataaggcag
       61 ctatgccggg tacgctcaag ctcaagccgg gattgtaggt gtatggtccc aggatgaggt
      121 ggtgcgggtt gatcttaaac gccgatgggt tcgttttcca ggcatcacag atgctttgga
      181 acggtgtttt ccacttcagt gccttgaggt gttttgaacc gcgccgggtt tgccggaggc
      241 tctgaacctt gagaggttgg agcaatggag aagtcaaagt cggcgaaccg gtattcggca
      301 gaggtacgtt cgcgtgcggt ccgaatggtt ttggagcacg gggcggatca cgcctcgcaa
      361 tgggctgcga tcggctcgat tgcggccaag attgggtgca cggcggagac gttgcgtgcc
      421 tgggtgcggc aggctgagcg cgaccagggg cgcaggcccg ggccgacaag cgaagaccag
      481 gcccgcgtca aagcgttgga acgcgaagtg cgcgagttgc ggcaggccaa cgagatcctg
      541 cgcaaggcgt cagcgtattt tgcgatggcg gaactcgacc gccggtccaa gccatgatcg
      601 gcttcatcga cgatcatcgc gctgcttacg gggtcgagcc gatctgccgc gtgctgccga
      661 tcccctcggc tggctttcca cctgcaatgc aggtggaacc tttgccaccc tcgccccgag
      721 cacctaccac gcgcacgccg cccaacgtgc cgatccatcc cgacgatccg cccgggccca
      781 gcgtgatgcg gccctgcagg ccgatataca gcgggtctgg gaggcgaact tccgggtcta
      841 tggtgtgcgc aaggtctggc ggcaactgcg gcgcgaagcg attgtggcgc cgcgctgcca
      901 ggtggcaagg ctgatgaagc gcatgggcct ggccggcgcg gtgcgtggtc gcacggtgag
      961 gaccacgatc agcaacctcg cggcgccatg cccgctcgac cgggtcaacc ggaagttccg
     1021 ggcgttggca cccaatgtgc tgtgggtgtc ggattttacg tatgtcgcca cctggcaagg
     1081 ctttgcttat gtggccttca tcatagatgt cttcgcccgg cgcatcgtcg gctggcgggt
     1141 atcgcgttcc gcccgcgcgg acttcgtgct ggatgcgctc gaacaggccc tgtgcgaccg
     1201 gcggccggtg cggcagggcg gcctggtgca tcacagcgac cgtggcgttc aatatgtcag
     1261 cattcgctat accgaacgct tggcagaagc cggcatcgaa ccctctgtcg gcagcatcgg
     1321 cgacagctac gacaacgcgc tcgccgaaac cattaacggt ctgttcaagg ccgaggtgat
     1381 ccaccgccgt ggcccgtggc gcagtgccga gacggtggaa ttcgccacgc ttgaatgggt
     1441 cgactggttc aataaccgac gcttacttga accgatcgga aatattccgc ccgccgaggc
     1501 ggaaacgcgc tactatgccc aagccaacga gcacgtcatg gcggcgtgac tcaaaccaaa
     1561 cagcctccgg caaacccggc gcggttcaat cagcggcccc gaagcgttgt tggcactggc
     1621 tacgcatctt gacgcaatgg ttgccactga ccgggccggc caaccggacg tc
//
LOCUS       DASWQN010000529         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1898368, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000529 DASWQN010000000
VERSION     DASWQN010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898368"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1282)
                     /locus_tag="VGC82_08830"
     CDS             complement(107..1282)
                     /locus_tag="VGC82_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0423410.1"
                     /translation="MLKPRGRHFFANPGPTNIPDSVLRALDRPSIDFNDQEFVPVYDE
                     AFAGVKRVLQTQQHLFMYNASGHGAWEASLTNLLSPGDTILVLESGYFSDEWAAMARG
                     HGLEVQVVAADWRRGVDVADVRRALAADTAHAIKAVCVVHNETATGLMLPLPAIRTAI
                     DAVGHPALFLVDTISSLGSLEFRMDAWKVDCVVGGSQKGLMLPTGLSFTAVSEKAMAA
                     HASAKLHRFYFDWTMMTNRTQKSFIGTIPVNVFYGLREALRLLEEEGLDNVVARHTRL
                     AEATRRAVQVWAGNNGPTLFCMSPDRYSNSVTAVMMPEGFNGDTLRKAVRDRFNVSLG
                     GGLGKLGGKIFRIGHLGDLNEPMLLGALAAVEMGLRIEGIPHGRGGVEAAMDFLAEA"
BASE COUNT          370 a          619 c          583 g          358 t
ORIGIN      
        1 ggccttggag aacaccgtac aagccaccat caggaggttc agggatgcgg cttgaagcgc
       61 ctttggtggc cggtcgaaac gctttatagg ccgcacccag cgacgcctat gcctcggcca
      121 ggaagtccat agccgcctcc acgccacccc ggccatgcgg aatgccttcg atccgcaggc
      181 ccatctccac ggcggccagg gcccccagca gcatcggctc gttcaagtcc ccaaggtgcc
      241 cgatgcggaa aatcttgccg cccaacttac ccaatccgcc gcccagcgaa acgttgaagc
      301 ggtcgcgaac cgccttgcga agcgtgtcgc cgttgaaccc ctccggcatc atcacagcgg
      361 taaccgagtt cgaatagcgg tccggcgaca tgcagaacaa tgttggcccg ttgtttccgg
      421 cccagacctg gaccgctcgc cgcgtcgcct cggccagcct ggtgtggcgc gcaaccacgt
      481 tatcgaggcc ttcttcctcc agcagccgca gggcttcgcg caacccgtag aagacgttca
      541 ctggaatggt gccaatgaag ctcttctgag tacggttcgt catcatcgtc cagtcgaagt
      601 agaagcgatg aagtttcgcg ctggcatgtg ctgccatcgc cttttcggac acggcggtga
      661 agctcaggcc cgtgggaagc atcaatcctt tctggctgcc gccaacgacg caatcgactt
      721 tccacgcgtc catgcggaac tcgagcgaac cgagcgagga gatcgtgtcc acgaggaaca
      781 acgccggatg accaaccgcg tcgatggccg ttcgaatggc cggcagcggc agcatcagtc
      841 cggtcgcggt ttcgttatgg acgacacaga ccgccttgat cgcgtgtgcc gtgtcggcgg
      901 ccaatgcgcg gcggacatcg gcgacatcga cgccacggcg ccagtccgcg gcgaccacct
      961 gcacctcgag cccgtggccc cgcgccatcg ccgcccattc atcggagaaa tagcctgact
     1021 ccagcaccag gatcgtgtcg cccggtgaca gcaggttggt caggctggct tcccaggcgc
     1081 catggccgga ggcgttgtac atgaacaaat gctgttgggt ttgcaggacg cgctttaccc
     1141 ccgcgaaagc ttcgtcataa accggcacga actcctgatc gttgaagtcg atgctggggc
     1201 ggtccagcgc ccgcaacaca ctgtcgggga tattggtcgg gccggggttg gcgaaaaaat
     1261 gtcgcccgcg aggcttcagc atgaacatga tcctatcggc tttctatcgc ggctttgtaa
     1321 accaaacgtc cgttctcgtc tcaagggcct gcggaacagc tcggcgcgcg cctactctag
     1381 gcgttcgact attgccacgg cggctgccgg ttttgaagcc gctgacgacg caccggtaga
     1441 tgacgcacag gtagatgtcg cggctggtat ttccttcttg ccgcgcatcg acagggctga
     1501 gctcgtccgg tgctacgccg gctgcccacg cgcggacccg ttccgacgac agattcaaag
     1561 cggctttagg tttcgctgac aaataaccgc ttcgagccgg ttgatcttgt gtcctgccca
     1621 gccgtgtgcc ggccatccac gagttgccgt attgaaacca aggcgtggcc ggccaaacgt
     1681 acgcgaaagg cgcggttaga tgatcggcga tccgcggatg gcctgaatcg cccgggacga
     1741 cgatcgcgat gccatactcg cgggcgagcg cgacgaagtc gggtgaccgt gaacggttgt
     1801 ggcaaacctc gatcccgatg gaggtcagat tggggctggc aatctcagtt ctcgccgcta
     1861 caccaggcga tcaggatagc acgcattgcg ctatggttca tcggtcccag ccgccgacgc
     1921 cagcctggat
//
LOCUS       DASWQN010000530         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2293863, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000530 DASWQN010000000
VERSION     DASWQN010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2293863"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1062
                     /locus_tag="VGC82_08835"
     CDS             313..1062
                     /locus_tag="VGC82_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0423411.1"
                     /translation="MLGRKKLITVFFITHGGRMFANKVIIVTGAAGNVGSALARVLAS
                     RGARVVLVDTIRDRLSAVKEALEGSGHLAYSEYDLTDSAASKALVAKVQSTCGRLDGV
                     GTTVGGFAMARLEDAGLDQWDAMFRLNVKTTWNIYQAAVPVIREGGGGALVGIGSAAG
                     LRGSAQMAAYSATKSAVMRLTESLADELRADRIRVNAVLPTTIDTPQNRAAMPGADTS
                     LWVQPSEVAEAMTFLLSDAASGVSGALLPVG"
     gene            1095..2111
                     /locus_tag="VGC82_08840"
     CDS             1095..2111
                     /locus_tag="VGC82_08840"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0423412.1"
                     /translation="MQTGSTASQRRLILPAVLFLTWVFSAGMTQAEDRVTFGLDWRAE
                     AEYGGYYQALANGLYARHGLNVSIQQGGPQINHMQLLIAGRLDFNLGGGRAIEFAQEN
                     LPFLAVAAIFQKDLAVLIAHPGQGNDSFAALKGKPIMIGSDTRNGWWRFLAAQFAYSD
                     SQIRPYTFNMAPFLADKNAIQQGYLSSEPYLIREKVGFDPVVLSVSDAGYLGYANILT
                     TSMKLVHDRPDVVQRFIDASIEGWYAYLYGDPMPGNRLIRQANPDMSADLIDFARKTM
                     IEHGTVDSGDAKTLGIGAMTDMRWKSFYQSMTEVGVYKPGIDISKAYTTQFVNKRVGM
                     PLER"
     gene            2133..>2567
                     /locus_tag="VGC82_08845"
     CDS             2133..>2567
                     /locus_tag="VGC82_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY0423413.1"
                     /translation="MTDRTPPPWKGLQTGRMPSFISYSQVERMVGSMLDGVMAWQPDA
                     VVAIVRGGLVPATMVSCMLSLPLFMVGWDRETSITSWVGSAANVKRLLVVDDCCTTGR
                     TMLSVEPALRHQEYECATLTVVHDPETTGYLPNFSHPMTELFR"
BASE COUNT          514 a          738 c          803 g          512 t
ORIGIN      
        1 cgattacgct gagcgggttc acgccggccg accttacgaa cggcaagctt tccatcagct
       61 acgggacgac agccgatctt cccaatttgc ccggaaccca cttcatgacg attcatggaa
      121 actgaagcat gtccatactg ggcggaatgt ccgggcgtgg gaacaagatg acaggccatt
      181 tctagcctgc acagtcaggc ggaatggtct gacggcccga tcctgaatga ctggctgccg
      241 gatcaggcca aaacggtaat cgctctcgcg aaatctcgtg ctgtttgcca gacgcggctt
      301 tttgctgtga caatgttggg ccgcaaaaaa ctgatcacgg tctttttcat cacgcacggg
      361 ggcaggatgt tcgccaacaa ggtcatcatc gtcacaggtg ccgccggcaa cgtcgggtcg
      421 gctctcgcga gagtgttggc ctcgcgcggg gcacgcgtgg tccttgtcga taccattcgc
      481 gatcgacttt ccgccgtgaa agaggcgctg gaaggaagcg gtcacctggc gtattcggaa
      541 tacgacctga ccgattccgc ggcatcaaaa gcgctggtcg ccaaggttca atccacctgc
      601 ggtcgcctgg acggtgtcgg cacgaccgtt ggcggttttg ccatggccag gctcgaggat
      661 gcgggattgg accaatggga cgcgatgttc cgtctgaacg taaagacgac ctggaacatc
      721 taccaggcag ccgtgccggt cattcgcgaa ggcggtggcg gtgctttggt gggaatcggt
      781 agtgcggccg ggttgcgcgg aagtgcccag atggctgcct atagtgcaac caaaagcgcg
      841 gtgatgcggc tgactgaaag cctggcggat gagctgcggg ccgaccgcat acgggtcaac
      901 gcggttctgc cgactacgat cgataccccc cagaacaggg cggcgatgcc gggggcggac
      961 acctcgctct gggtccaacc gtccgaagtc gccgaagcca tgaccttcct tttgtcagac
     1021 gccgcgagcg gcgtcagcgg ggcattgctg cctgttgggt agcactggag tcaaatttac
     1081 ggggtaacaa cgtcatgcaa acggggtcca ccgccagtca acggcggctc attctaccgg
     1141 ccgttctttt cctgacttgg gtattctccg ccgggatgac ccaagcagag gatcgggtca
     1201 ccttcggtct ggattggcgg gcggaggcgg agtacggcgg ctattatcag gcgcttgcca
     1261 acggtctcta cgcccgccat gggctgaacg tcagcattca gcagggcggt ccccagatca
     1321 accacatgca gcttttgatc gccggccgac tggacttcaa tctgggtggc ggccgtgcga
     1381 tcgagttcgc ccaggagaat ctaccgtttc tcgcggtggc ggcgatcttt caaaaggatc
     1441 ttgcggtgct gattgcccac cctggacagg gcaacgacag cttcgccgcg ttaaagggca
     1501 agccgatcat gatcggaagc gatacgcgca atggctggtg gcgcttcctg gcggcgcaat
     1561 tcgcttatag cgacagccag atccggccgt acacgttcaa tatggcgccg ttcctcgcgg
     1621 acaagaatgc gatacagcag ggttacctca gcagcgagcc ataccttatt cgggaaaagg
     1681 tcggtttcga tccagttgtc ttgtcggtgt cggacgccgg atacctgggc tacgcgaaca
     1741 tcctcaccac cagcatgaag ttggtccacg accgcccgga cgtggtgcag cgcttcatcg
     1801 acgcatcgat cgaaggctgg tatgcttacc tctacggtga cccgatgccg gggaacaggc
     1861 tgatcaggca ggccaatccc gacatgtcgg ccgacctgat agatttcgct cgcaagacaa
     1921 tgatcgagca tggtaccgtc gactcgggcg atgccaaaac gcttggcatt ggcgcgatga
     1981 ccgatatgcg ctggaagtcc ttctaccagt cgatgactga agtcggggtt tacaaaccgg
     2041 gcatcgacat ctccaaggcc tataccacgc agttcgtgaa caagcgcgtc gggatgcccc
     2101 tggaacgtta acccggtgat ctggaagtaa agatgaccga ccgcacgccg cctccctgga
     2161 aaggtctgca gaccggccgg atgccaagct tcatctcgta cagccaggtg gagcgcatgg
     2221 tcggatcgat gctcgacggc gtcatggcct ggcagcctga tgccgtcgtg gcgatcgtgc
     2281 gcgggggact tgtgccggcc accatggtgt cctgcatgct ctcgttgcca ttgttcatgg
     2341 tgggttggga tcgggaaacc agcatcacaa gttgggtggg ctcggcggcg aacgtgaaac
     2401 gcctgctggt cgtggacgat tgctgcacga ccggacggac gatgctttcc gtcgaaccgg
     2461 cactacgcca ccaggaatat gagtgtgcga cgctgacggt cgtgcacgat ccggagacga
     2521 ccggctactt gcccaacttc tcgcacccaa tgacggagtt gtttcgc
//
LOCUS       DASWQN010000531         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_578553, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000531 DASWQN010000000
VERSION     DASWQN010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578553"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..998)
                     /locus_tag="VGC82_08850"
     CDS             complement(48..998)
                     /locus_tag="VGC82_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0423414.1"
                     /translation="MVEENQPMLALVNTPNGSAPIELREVAEPSLAHHEALVEVRAFS
                     LNRGELSSFARNKEGWIPGQDVAGVVLRQAADGNGPPTGARIVALVDEFGWAERAAVP
                     NHRMATLPVEVSFAQAATLPVAGLTALRTLRHGGSLVGRRVLVTGAAGGVGTLAVQIA
                     ARSGAYVTAVVGRTERGAGLSELGATEIVEGIENVQGRFALILESGGGASLAKAIRLV
                     EPRGTVVVYGNSSTEPTTISFADFRGAPNARIQSFSYFTSEAEERFAPDLALLVSLIA
                     DGSLKPQIGAERDWRDILQVADQLRDRRVSGKAVFRVGAR"
     gene            complement(1134..1940)
                     /locus_tag="VGC82_08855"
     CDS             complement(1134..1940)
                     /locus_tag="VGC82_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY0423415.1"
                     /translation="MQISAGYDITYQCPQPTPMLLVLSVHPSRMPDIVGPHQISFDPP
                     IGATEYRDGFGNICHRIVAPAGSVRISTRFMISDPGTPDAVVADAAQHAIQDLPDEVL
                     VYLLGSRYCETDRLTQLAWSLFSNTPEGWPRVQAICDYVHDRIEFGYHHADPERSAFA
                     GHADRRGVCRDFAHLAVTLCRCMNIPARDCTGYLGDIGIPPVDAPMDFSAWFEVYLGG
                     HWHTFDARHNTPRIGRILMARGRDATDVALSTIFGPSMLIGFNVVTDEVG"
     gene            2301..>2503
                     /locus_tag="VGC82_08860"
     CDS             2301..>2503
                     /locus_tag="VGC82_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423416.1"
                     /translation="MSKTTTLAIVSSMAFFSLATTVVQAQQAPPQSPDMTFFVTSTGL
                     GKGADLGGLAGADRQCQALAQAAG"
BASE COUNT          465 a          799 c          793 g          446 t
ORIGIN      
        1 aaccgaggct gtcaggaaca aaggcaggaa agctggccag tccaccgcta tcgcgctccc
       61 acccggaaca ccgccttgcc cgagactcgc cggtcgcgca gttggtcggc gacctgcagg
      121 atgtcccgcc agtcgcgctc cgcgccgatt tgcggcttca acgatccgtc ggcgatcagc
      181 gataccagca gtgcaaggtc cggtgcgaaa cgttcctcgg cttccgaggt gaaatagctg
      241 aaactctgga tccgcgcgtt cggagcaccc cggaaatcag cgaagctgat ggtcgtcggt
      301 tcggtggatg agttgccgta cacaaccact gtgccccgcg gctccacgag gcgaatcgcc
      361 ttggccagtg atgcgccgcc tcctgattcc aggatcagcg cgaaacggcc ctggacattt
      421 tcgatgccct cgacgatttc cgtcgcgccg agctcgctca ggcccgcgcc acgttctgtc
      481 cgtccgacaa cggcggtcac ataggctccg gaccgagcgg cgatttgtac cgccagcgtt
      541 ccgacgccgc cagccgcccc ggtgaccagc acccgccgcc cgaccaatga tccgccgtgg
      601 cgtaaggtcc ggagtgccgt cagcccagcc accggcaacg tcgccgcctg ggcaaagctg
      661 acctccacgg gcagcgtcgc catcctgtgg ttcggtaccg cggcgcgctc cgcccaaccg
      721 aactcgtcca ccagcgcgac gatgcgggct ccggtcggcg ggccgttgcc gtcagccgcc
      781 tggcgcagca cgacgccggc gacatcctgc cccggaatcc agccctcctt gttgcgtgca
      841 aacgagctga gttcgccgcg attcaacgaa aacgcccgca cctccaccag cgcttcgtga
      901 tgggctaggc tgggttcggc gacttcgcgc agttcgattg gggccgatcc gttgggcgtg
      961 ttcaccaggg caagcatcgg ctggttctcc tcgaccatgt ggggcgtccg gcgcgtgagc
     1021 cggacgcggc gtctcgtcac gggctaatgc ccatggctgg acccacaccg ttcggccggt
     1081 cggcggcatc aggccactgg tcgaacgccg cggcaagatg gctggccccg ggtttagccg
     1141 acttcgtcgg tcacgacgtt gaacccgatc aacatgcttg gtccgaagat cgtggacaat
     1201 gccacgtcgg tcgcgtcacg tccccgcgcc atcaggatcc ggccgatgcg aggcgtgttg
     1261 tgacgggcgt cgaaggtgtg ccaatggccg ccgagataga cctcgaacca ggcgctgaaa
     1321 tccatcggtg cgtccaccgg cgggatgccg atgtcaccga ggtagccggt gcagtcgcga
     1381 gccgggatgt tcatgcagcg gcacagcgtg acagcgaggt gggcgaagtc tcggcacacg
     1441 ccgcgacggt cggcgtgacc ggcgaacgcg ctgcgttcag ggtcggcgtg atggtagccg
     1501 aactctatcc ggtcatggac atagtcgcag attgcctgaa cccgaggcca gccttccggc
     1561 gtgttgctga acagcgacca ggcaagctga gtgagccgat cggtttcgca gtagcggctg
     1621 cccagaagat agaccagcac ctcatcgggc agatcctgga tcgcatgttg cgcggcatcg
     1681 gcaacgactg catcaggcgt gccgggatcg ctgatcataa accgcgtgga gatcctcacc
     1741 gatcctgccg gcgccacgat ccgatggcag atgttgccaa agccgtcgcg gtattcggtc
     1801 gcgccgatcg gcgggtcgaa gctgatctga tgcggcccga ctatgtcggg catccgtgag
     1861 ggatgcacgg ataaaaccag cagcatcggg gtcggctgag ggcactggta ggtaatgtcg
     1921 tagccagcac tgatttgcac gaatgtgtat ctccaacgcg attacttgag gcagcataag
     1981 ggcgtcgacg agccgctcgg ccgcgctcaa ccgaagtctg cacaaatgtc atgcactcga
     2041 agcgcctact ccacacaaac acgtgagctt gctcgttttc gccgcattgg aggccgcgaa
     2101 gcgatctgcc tgcggaagtg tgttcagcca tggccgagat ggcttatcgg cttcacgtac
     2161 agcctacggc agtttgtact acagcgtcgc aatcacccga tgcgggagtc cgcaaaagac
     2221 atttacatcg actgggttca tgctagtttc gttatcaagg agtgcgggac ataacccgcg
     2281 gacacacggg gagatcgatc atgtccaaaa caacgaccct ggcgattgtt tcgtcaatgg
     2341 ccttcttttc gctggctaca accgtcgttc aggcgcagca ggcgccgcca cagtcacccg
     2401 acatgacatt tttcgtgacc agcacgggcc ttggcaaggg cgcggatttg ggtggtctgg
     2461 cgggggcgga tcggcagtgc caggcattgg cacaagccgc tgg
//
LOCUS       DASWQN010000532         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2425542, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000532 DASWQN010000000
VERSION     DASWQN010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2425542"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VGC82_08865"
     CDS             complement(<1..685)
                     /locus_tag="VGC82_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEY0423417.1"
                     /translation="MRVSHPFGLKLSMESAQQIAEAALRQQPADGVSLVLTPNIDHIA
                     TIRRSPALARAYRNAAHIVCDGWPVQTYARWCGLPVGRVTGCEITAKLMRMAPYATSH
                     RLFFAVDSATTEHAVRDWAMKNQVACDVSVPPFGFERDAEYCRRLVAAINKHQTTVLI
                     MAVGAPRSEVFVDTYRSILPPCWAFCVGQAVKVELGLVTRASPRWQSLGMEWLWRLRQ
                     EPARLTRRYL"
     gene            complement(688..>1585)
                     /locus_tag="VGC82_08870"
     CDS             complement(688..>1585)
                     /locus_tag="VGC82_08870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0423418.1"
                     /translation="KPDILLSNVDLNNVAALLAKGISLSKTKIVICQHNPISSNFVMG
                     ENWLYRYIALSYRVLAPLICRAVAVSEGVAAELGNIAGLPPERVLTINNPVVGPDFQA
                     RSEEPADHPWFHQPQHPVFLTAGRLVTQKDHATMIRALAIHRQHHDSRLIILGSGPLR
                     PVLVELVARLGLTGCVDFLGFHSNALPYIRQADAFLLSSRCEGFGNVIVEALGCGTPV
                     ISTRCEHGPAEILDNGRYGVLVEPRDPLALAAAMDQVATLREQFPAEMLRQRAGDFSY
                     AACASRYMAVFKALAPNCAWAV"
BASE COUNT          318 a          512 c          479 g          276 t
ORIGIN      
        1 caagatagcg tcgggtcagc cgagcgggct cctggcgaag ccgccatagc cattccatgc
       61 caagagactg ccaccgaggc gatgcgcgtg tgaccaagcc aagctccacc ttgaccgcct
      121 gaccgacgca aaaggcccaa cacggcggca ggatggagcg ataggtatcg acgaacacct
      181 cgctgcgcgg ggcgccgacc gccatgatca gcacggtcgt ctgatgtttg ttgatggcgg
      241 ccaccagccg ccggcagtac tcggcatcgc gctcgaaccc aaacggcggc accgatacat
      301 cacacgccac ctgatttttc atcgcccagt cacgcacggc gtgttccgtt gtcgcgctgt
      361 ccacggcgaa gaacagccgg tgtgatgttg cataaggtgc catacgcatc aacttggccg
      421 tgatctcgca ccccgtgacc cgcccaacgg gaaggccgca ccaacgggcg taggtttgca
      481 caggccatcc gtcgcagacg atatgagcgg cattccgata cgcccgagcc agggcggggg
      541 acctgcgaat ggtcgcgata tggtcgatgt ttggtgtcaa cactagcgag acaccgtcgg
      601 ccggctgttg ccgaagcgcg gcctcggcga tctgctgcgc ggactccatg ctgagtttca
      661 acccgaacgg gtggctgacg cgcatgttca taccgcccag gcgcagttcg gtgccagtgc
      721 cttgaacacc gccatatagc gcgacgcaca ggcggcatag ctgaagtcgc cggcccgctg
      781 tcgcagcatt tccgcgggga attgctcacg cagcgttgcc acctgatcca tcgccgccgc
      841 caacgccaac ggatcccgcg gttccaccag cacgccgtag cggccgttat ccaggatctc
      901 cgccggtcca tgctcgcagc gcgtggatat gaccggggtg ccgcagccta aggcctcaac
      961 gatgacgttg ccaaacccct cgcagcggga gcttagcagg aacgcatcgg cctggcggat
     1021 atagggcagg gcattggagt gaaaacccag gaagtccacg caaccggtca atccaagcct
     1081 ggcgaccagt tccacgagta ccggccgcaa cggaccgctg cccagaatga tcagccggct
     1141 gtcatggtgc tgccggtgaa tcgccagcgc ccgaatcatc gtcgcatgat ctttctgtgt
     1201 caccaggcgc ccagccgtca agaagaccgg atgctgcggc tgatgaaacc agggatgatc
     1261 ggcgggttcc tcactccggg cctggaagtc gggtccaacc acaggattgt tgatggtcag
     1321 aacgcgctcc ggcggtaatc ccgctatgtt tcccaattcc gccgccaccc cttcggaaac
     1381 cgccaccgct cggcagatca acggcgccag cacccggtag gacagggcga tataccggta
     1441 tagccagttc tctcccataa cgaagttgga cgagatcggg ttgtgctggc atatcacgat
     1501 cttcgtcttg ctcaggctga tgcctttggc cagcaacgcg gcgacgttgt tcaggtcgac
     1561 gttggacagc aggatgtcgg gcttt
//
LOCUS       DASWQN010000533         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2558660, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000533 DASWQN010000000
VERSION     DASWQN010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2558660"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VGC82_08875"
     CDS             <1..741
                     /locus_tag="VGC82_08875"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEY0423419.1"
                     /translation="ITSSDGQAVAGHALAYDQESGFGLVQALGKLDVPTLEMGDSDRL
                     KVGEQAVLAAGGGRHHSIETRIVGRQEFAGYWEYVLDDALFTAPAHPFWGGCGLIGTD
                     GKLMGIGSLILQQGDGKGRRLDMNMIVPIGLLAPIMDDLLSYGRVNRPARPWLGMYAT
                     EADETLVVGGLADNGPAEQAGVRAGDKILTVNDEEIPDLAGLWRRVWSSGSAGAEVRL
                     RIERDGSPKRVAVRSADRASFLKSPKLH"
     gene            762..1430
                     /locus_tag="VGC82_08880"
     CDS             762..1430
                     /locus_tag="VGC82_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0423420.1"
                     /translation="MSHDAPTRLRGAIVPVTPFQQNCAILWDDASKIGMVVDPGGDVD
                     RILAGIDQTGVVIEKIVLTHGHLDHAGGAAGLRDALQARYGASVPIEGPDRRDEFLLD
                     GIAEQAAAYGFEASNVTPDRWLTEGEVLTLGPHRFDVLHCPGHTPGSLVFVNNEARFA
                     LVGDVLFQGSVGRTDFPYGDHAALIDAIKTKLLPLGDDVSFICGHGPGSTIGAEKASN
                     PFLR"
     gene            1427..2659
                     /locus_tag="VGC82_08885"
     CDS             1427..2659
                     /locus_tag="VGC82_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY0423421.1"
                     /translation="MTPARQLALFAATAEPTLPTDFVDGLRRFTFDGAKTVEFEEAGL
                     RYLVNAFWTAGQRRGHSIHEISYRACFKSQLPGFFIERLTNPGDRVYDPFSGRGTTAV
                     QAALLGRAPAANDVNPLSGMLSGPRLDPPRVEAVRLRLAEIDLAREVGEPGEADLLAF
                     YHPDTLRQIKALRVWLLERGEAIDPVDAWIRMVAINRLTGHSTGFFSVYTMPPNQAVS
                     ALSQRKINQAREQVPPPRDVRAIILKKTLSLLSDGVPPPHPPGILLTGPAERTPELAD
                     GSVDLIVTSPPFLDVVDYEGDNWLRCWFAGIDPAGVRIDKHKDIAAWERFVRRGFREF
                     ARVVRPGGFVAFEVGEVRGGSVLLERHVATAIEGLPFDVLGVMVNQQNFTKTANCWGV
                     GNNRGGTNTNRIVMAIRR"
BASE COUNT          475 a          896 c          994 g          499 t
ORIGIN      
        1 atcacctcgt ctgatggcca ggcggtggcg ggccacgcgc tggcctatga ccaggaaagc
       61 ggtttcggcc tggtccaggc gctgggcaag ctggacgtgc cgacgctgga gatgggcgac
      121 tcggatcggc tgaaggtcgg cgagcaggcg gttctggcgg cgggtggtgg ccgtcatcac
      181 tcgatcgaga cccgtatcgt tggtcgccag gaattcgccg gctactggga atacgtgctg
      241 gacgacgcgc tgttcaccgc gccggcgcat ccgttctggg gtggctgcgg cctgatcggg
      301 acggatggca agctgatggg catcggctcg ttgatcctgc agcagggcga cggcaaggga
      361 cggcggctgg atatgaacat gatcgtccca atcggcctgc tggcgccgat catggacgat
      421 ctgctgagtt acgggcgggt caatcgtccg gcgcgtccct ggcttgggat gtacgccacc
      481 gaggcggacg aaacgctggt ggtcggggga ttggccgaca acggtccggc tgaacaggcg
      541 ggcgtgcgtg ccggcgacaa gatcctgacc gtgaacgatg aagaaatccc tgatctcgcc
      601 gggttgtggc gccgggtctg gtcgtccggc agcgcagggg ccgaggtgcg gctgcggatc
      661 gagcgcgacg gttcgcccaa gcgcgtggca gtacgatcgg ccgaccgggc cagtttcctg
      721 aagtccccaa agctgcactg atatgccaaa gctgcgctga tatgagccac gatgcgccga
      781 cgcggttacg cggggcaatc gtcccggtca cgccgtttca gcagaactgc gcgatcctgt
      841 gggacgatgc cagcaagatc ggcatggtgg ttgatcccgg cggcgacgtc gatcgtatcc
      901 tggccggaat cgaccagacc ggcgtggtga tcgagaagat cgtgctgacg cacggccatc
      961 tggatcacgc cgggggtgcc gccggcctgc gcgacgccct tcaggcacgg tatggcgcca
     1021 gcgtcccgat cgaggggccg gatcggcgcg atgaattcct gctcgacggc atcgccgaac
     1081 aggccgccgc ctacgggttc gaggcgagca atgtcacacc cgaccgttgg ctgaccgagg
     1141 gcgaggtgct gaccctcgga ccgcatcgct tcgacgtgct gcactgcccc ggacacacgc
     1201 ccggatcgct ggtcttcgtt aacaacgaag cgcgcttcgc gttggtgggc gacgtgctgt
     1261 tccagggttc ggttggacgc accgactttc catacggcga ccatgccgcc ctgatcgacg
     1321 ccatcaagac caagctgctg ccgcttgggg acgatgtttc gttcatctgc gggcacggcc
     1381 ccggctcgac gatcggtgcg gagaaggcca gcaacccatt tctgcggtga cccccgctcg
     1441 ccagcttgcc ttgttcgcgg ccaccgccga accaacgctg ccgaccgatt tcgttgacgg
     1501 tttacggcga tttaccttcg acggcgccaa gaccgtcgag ttcgaggaag cggggttgcg
     1561 ctatctcgtc aacgcattct ggaccgccgg ccagcgccgc gggcacagca tccacgagat
     1621 cagctaccga gcctgtttca aatcgcagct tcctgggttt ttcatcgagc gcctgaccaa
     1681 tcccggcgac cgggtttacg acccattttc cggtcgcggc acgacagcag ttcaggcggc
     1741 attgctgggc cgtgcgccgg cggcgaacga cgtcaacccg ctgagcggga tgctgtcggg
     1801 cccgcgcctc gatccgcccc gggtcgaggc ggtgaggctc cggctggcgg aaatcgactt
     1861 ggcacgcgaa gtcggcgaac cgggggaagc cgatctcctg gcattctacc atccggacac
     1921 cctgcgccag atcaaggctt tgcgcgtctg gttgctggag cggggcgagg cgatcgatcc
     1981 ggtggatgcc tggatcagga tggtcgcgat caatcggttg accggccact ccacggggtt
     2041 cttctcggtc tataccatgc cgccgaacca ggctgtctcc gccctgtcgc agcgcaagat
     2101 caaccaggcg cgcgagcagg tgccgccgcc gagggatgtg cgggcgatca tcctgaagaa
     2161 gacactcagc ctgctgtcgg acggtgtgcc gccgccgcac ccgccgggga ttctgttgac
     2221 cgggccggcg gaaaggaccc ccgaactggc tgacgggtcg gtggacctga tcgtgacctc
     2281 gccgccgttc ctggatgtgg tcgattatga gggcgacaac tggctgcgct gctggttcgc
     2341 cggcatcgat ccggcaggcg tgcggatcga caagcacaag gacatcgcgg cgtgggaacg
     2401 cttcgtgcgc cgtggcttcc gcgagttcgc ccgtgtggtc cgccccggcg ggttcgtggc
     2461 gttcgaggtc ggcgaggtgc gcggcggcag cgtgctgctg gaacgccatg tcgcgacggc
     2521 gatcgagggc ctgccgttcg acgtgctcgg cgtgatggtg aaccagcaga acttcaccaa
     2581 gacggcgaat tgctgggggg tcggcaacaa ccgcggcggc accaacacca accggatcgt
     2641 catggcgatc cggcggtagt cttgcatccc atcacttgtc gcacccctgc cgctgacgag
     2701 gccggcggca ctacgagttg tctccgatag ggggtttcgg acgggtggat cggaggggca
     2761 cgccgaatcg agcgccatcc cacccaccca agaacgcgcc agcctactgg agccagccta
     2821 ctggaaatgt actaacgagc cgttgcgctg ttccagcggc aggc
//
LOCUS       DASWQN010000534         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_183658, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000534 DASWQN010000000
VERSION     DASWQN010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183658"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..813
                     /locus_tag="VGC82_08890"
     CDS             262..813
                     /locus_tag="VGC82_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY0423422.1"
                     /translation="MPPRPRRLSEADQALWAAYASQLAPLRGRVSRKPLQIQPLVAEP
                     APPATPAPPPNLKFAAPAIKPRRMIPPLSVGDQPGGIDKSTWQRFRTGKLQAGRKLDL
                     HGMTTQRAFQALLTFLRNAHADQVRCVEVITGRGSGETGGIIRREFSHWLNLPEIRPL
                     ILGAAHPHTRNQGSVRLILRRIR"
     gene            810..1229
                     /locus_tag="VGC82_08895"
     CDS             810..1229
                     /locus_tag="VGC82_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0423423.1"
                     /translation="MTPFGARLRALRADRGVTLKHLAEALQVSAAYLSALEHGKRGSP
                     GTGLVHQVNEYFGLIWDDADELSRLARLSNPRVTVNTAGLTPEQTSLANRVAQMIHRL
                     PPETVAALHAVLDTATVRPKPRLRRVATSRSGERRLG"
     gene            complement(1278..1574)
                     /locus_tag="VGC82_08900"
     CDS             complement(1278..1574)
                     /locus_tag="VGC82_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY0423424.1"
                     /translation="MARVSRRMHPGRVLREEYMEPMRLDATGLADALGVARCQIVPVI
                     DNQEPMTSDLALRLARCFRTTPQFWLGLQTAYDLAMAQAQFGAEIDARVQPFTA"
BASE COUNT          300 a          525 c          527 g          300 t
ORIGIN      
        1 catgataccg gcatcttctc ctgggactat ctgcatgagc ttggccgcga gcatgaccat
       61 cgctgggccg gatacctgga cgcacttgca tcgcggggac tatcgcgcga tccgcgatag
      121 ctgcgtgtcg gtcgcgggac gcaatgacga aaacggtttg gcatcgcttg gactattggc
      181 ccgtggacca ctggcttgtg gacgattggc ccgtgccatg ttggccggcc acgcttttca
      241 tgacgccgag ggaaatagct tatgccgccc cgccctcgcc gtttgagcga agccgaccag
      301 gcactttggg cagcgtacgc cagccaactc gcgcctttgc ggggccgagt cagccggaag
      361 ccgctgcaaa tccaacccct ggtggcggaa ccagcaccgc cagccacgcc ggcgccgcca
      421 ccgaacttga agtttgccgc accggcaata aagccccgcc ggatgatacc gccgctgagc
      481 gtgggcgatc agcccggtgg aatcgacaag tccacctggc agcggtttcg caccggcaag
      541 ctacaagccg gccggaagct cgacctccat gggatgacga cgcagcgggc ttttcaggcc
      601 ttgctgacct ttctgcgcaa cgcccatgcc gatcaggttc gttgcgtaga ggtcatcacc
      661 ggtcgtggta gcggggaaac cggcggtatt attcgccgcg agttctcgca ctggctcaac
      721 ctgccggaaa tccgtccgct gatcctgggg gccgcccatc cgcacacacg caatcaaggt
      781 tctgtccgct tgatactgcg gcggattcgc tgaccccatt cggcgcccgg ctacgtgcct
      841 tgcgggctga ccgcggcgtg acgctgaagc acctggccga agcgttacag gtttcagctg
      901 cctatttgtc agccttggaa cacggcaagc gcgggtcgcc gggcaccggg ctggtgcacc
      961 aggtcaatga gtatttcggg ctgatctggg atgacgcaga cgaattgtct cggttggcgc
     1021 gcctgtcgaa cccgcgcgtg acggtgaaca ccgccgggtt gactccggaa caaacttcgt
     1081 tggcaaatcg ggttgcgcag atgatccacc gcttgccgcc ggagacagtg gcagcgctgc
     1141 acgcggtgct ggataccgca acggttcggc cgaagccgcg tttgcggcgg gttgcaacat
     1201 cccgatcagg cgagcggcgc ctggggtagg acccggggtg ggacatggtc aggcctcaag
     1261 gcgtgaatgg tcaggcctca agccgtgaaa ggctgaaccc gcgcgtcgat ctcggcacca
     1321 aactgggctt gggccatcgc caggtcatag gccgtttgca gtcccagcca aaattgtggg
     1381 gtggtgcgga agcagcgcgc gaggcgcaac gcgaggtccg acgtcatcgg ctcctgattg
     1441 tcgatcaccg gcacgatctg gcaccgcgcg acacccaacg catcggcaag gccagtcgcg
     1501 tcgagtcgca tcggctccat gtactcttct cgcagcaccc ggcccgggtg catcctgcgg
     1561 ctcacgcgtg ccatcggtaa cctatcgggt tttccatccg gacccatgaa tacaggcggg
     1621 tcgtggcagc aacatggcaa gattatcccg ac
//
LOCUS       DASWQN010000535         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_183704, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000535 DASWQN010000000
VERSION     DASWQN010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183704"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1477
                     /locus_tag="VGC82_08905"
     CDS             200..1477
                     /locus_tag="VGC82_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY0423425.1"
                     /translation="MAEASKKPRRMLLRLLGPGLVTGAADDDPSGIATYSQAGAQFGY
                     GLLWTVFLTTPFMIAIQLVSAQIGRVTGKGLAANVMELAPRWLVLALVSLLVAANTFN
                     IAADIAAMGEALSLVIGGLNHEHALIFASTSTLLQVFVPYRRYAPVLKFLTLTLFAYV
                     ATALTVHIPWSAALLAAIWPTPVINANYFLMVVAVLGTTISPYLFFWQASQEVEEMNQ
                     GKIDPPLRELGRGGHRELDRIKVDTIVGMIFSNVIAFFIILTTAAVLNANGITNINSA
                     TEAAEALRPLAGDFTFLLFALGIIGTGMLAIPVLAGSAAYGVSEAFGWPATLEAKAPD
                     AVGFYAIIAAATVIGFGLGFTGIDAIHMLVWSAVLNGIVAVPIMAMMMVIVANSQLMG
                     KFRARPRLIALGWLGTALMAVAVIALFWSFLAG"
BASE COUNT          274 a          498 c          490 g          344 t
ORIGIN      
        1 aaatctttcc ctgaaacatt cgcggtcttt ccccggcaat caagaaaatt cgagtcttgc
       61 tcgaacaggt ctcctctgcc gctgcggcgc aaaacttgag agctgaggga aagggccgag
      121 gggattcggg tctgccggca aaaaccacta ttgtgcgggg agcgaacgca cctgattcga
      181 ctcccgcgac ggggatttca tggccgaagc atccaaaaag ccccgaagga tgctgctgcg
      241 attgctcggt cccggactgg tcaccggcgc cgccgatgac gatccgtccg gcattgccac
      301 ctattcgcag gccggagcgc agtttggcta cggtttgctc tggacggtgt tcctgacgac
      361 gccattcatg atcgccatcc agctggtgag tgcgcagatc ggcagggtga ctggcaaggg
      421 gctcgccgcc aatgtgatgg agctggcgcc gcgctggctg gtcctggcgc tggtgtccct
      481 tctggtcgct gccaacacat tcaatatcgc cgccgacatc gctgcgatgg gagaagcgct
      541 ctcgctggtc atcggcggcc tcaatcacga gcatgccctg attttcgcat cgacttcgac
      601 tctgctgcag gtcttcgtac cgtatcgccg ctatgcgccg gtgctcaaat tcctgacgct
      661 cacgctgttc gcctatgtcg ccaccgccct gaccgtgcac ataccctgga gtgcggctct
      721 gctggcggcg atctggccaa caccggtcat caacgcgaat tacttcctga tggttgtcgc
      781 cgtgctcggc accacaatca gcccgtattt gtttttctgg caggcctccc aggaggtcga
      841 ggagatgaat caaggcaaga tcgacccacc gcttcgcgag cttgggcgag gaggccatcg
      901 ggagctcgac cgcatcaagg tagacacgat cgtgggaatg atcttctcca acgtgatcgc
      961 gttcttcatc atcctgacca ccgcggcggt cttgaacgcc aacggcatca ccaacatcaa
     1021 ttcagcgacc gaggccgctg aagcgctgag gccgctcgcg ggcgatttca cgtttctgct
     1081 ttttgccctg gggatcatcg gcaccggcat gcttgcgatc ccggtattgg ccggctcggc
     1141 cgcctatggc gtatccgaag cattcggatg gcccgcgacg cttgaggcaa aggctccgga
     1201 cgccgtcggg ttctatgcga ttatcgccgc cgccaccgtc atcggatttg ggcttggctt
     1261 taccggcatc gacgccattc acatgctggt ctggagcgcc gtgctgaacg gcattgtcgc
     1321 cgtcccgatc atggcgatga tgatggtgat cgtggccaat tcgcagttga tgggaaagtt
     1381 cagggccagg ccacggctga tcgccctggg ttggctcggc accgcgctca tggcggtcgc
     1441 cgtgatcgcg ttgttctggt ctttcctggc gggataggat tgctgcgagc aaagccgccc
     1501 gttctcgacc tggtcgtacc ggccaaatag gccgggccga cggctggccg ccgcgccgcc
     1561 ggctcgatct gggcgtccag tttcccggta gggttgtccc gcctcg
//
LOCUS       DASWQN010000536         2467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_711084, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000536 DASWQN010000000
VERSION     DASWQN010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711084"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..607)
                     /locus_tag="VGC82_08910"
     CDS             complement(125..607)
                     /locus_tag="VGC82_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0423426.1"
                     /translation="MAKITFPVEASHIMMFARAIGDTNPVYHDAAAAKDTEAGGLIAP
                     PSFAQAVAQFDPDYNLRPKPGQKWFGSAKGPSGIEGKPTSSGGLHAEQHYEYHRHVRP
                     GDVLTAETKPGKVWERESKRAGKLTFRERVTEYRDQNGELVITARGVGVTTERPVDQG
                     "
     gene            680..1504
                     /locus_tag="VGC82_08915"
     CDS             680..1504
                     /locus_tag="VGC82_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0423427.1"
                     /translation="MRRSVRRLSGRPSARAALDAASIPTFSGPRPMAGSAEAIRFLQA
                     PGWLPNGRRVYAVGDIHGCRDKLIALHGLIAADLAARPARSALLVHLGDYIDHGPASA
                     GVIALLAAAPVIPNIAVVNLLGDHERMLLDGLGGDRAAATDWLWAGGKAALESWGLTP
                     DLPREEWEAALPASHIAWLRSLVLTHREGNYLFVHAGIRPGVPLPDQSRDDLLTMRQP
                     FLSTEQDLGVVVVHGHSSSPSVSIAANRIGLDTGAGSGGKLTCAILEDDLVGLLAV"
     gene            complement(1578..>2467)
                     /locus_tag="VGC82_08920"
     CDS             complement(1578..>2467)
                     /locus_tag="VGC82_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEY0423428.1"
                     /translation="TLSADASRTLIGDDEHGWSENGIFNFEGGCYAKVINLSREAEPE
                     IWAASNRFGAVLENVVGDRHGNLDLADDSLTENTRSCYPVEFIPNVNLNGRGGQPKNV
                     FMLTCDAFGVLPPISRLTPAQAMYHFLSGYTAQVAGTEKGLGREPKATFSTCFAHPFL
                     PRPPQVYGRMFADLIAKHGATCWLVNTGWSGGKFGVGKRMSIRHTRALLRGALDGSLT
                     QMEFHKEPFFGLAIPAHVPGIPDEVLDPRRSWAEKTAYDDMAKHLIERFEENFAAYED
                     GVGEDVKAVAIRAAAAVGN"
BASE COUNT          382 a          771 c          820 g          494 t
ORIGIN      
        1 gtagtcgccc gatgcgcccg catactgcac gatttgcgtg cgcgacaggt tttccaccac
       61 gcgttcgcta tgggtgtcgc cgaccttcag ggcggatgct ttgagtgcca ttgttattct
      121 cccctcagcc ctgatccacg ggtcgttcgg tggtaacgcc cactccccgc gcggtgatca
      181 ccagctcgcc gttctggtcg cggtattcag tgacacgctc gcggaacgtc agcttgccgg
      241 cgcgcttgct ttcccgttcc cagaccttgc caggcttggt ttccgccgtc agcacatcgc
      301 ccggccgcac atgccggtgg tattcgtagt gttgttcggc atgaaggccg cccgaggatg
      361 tcggcttacc ctcgattcca ctcggaccct tggctgaccc gaaccatttc tggcccggct
      421 tggggcggag gttatagtcc ggatcgaact gcgcgaccgc ctgtgcaaaa ctgggcggag
      481 cgatcaatcc gccggcttcg gtgtctttcg ctgccgccgc gtcgtgatac acaggattgg
      541 tgtcgccgat cgcgcgggcg aacatcatga tgtggctcgc ttcgacgggg aatgtgattt
      601 tggccaaggg ttccctccgt ttgcttttac cccgacaggt tagcagggtt gcggaaagaa
      661 gaaacaggcg gttcggcgca tgaggcgttc tgtgcggcgt ctgtcgggcc gccccagtgc
      721 gcgggcagcc ctggatgccg cttcgatacc gactttcagc gggccgcgtc cgatggccgg
      781 gtcggccgaa gcgatcaggt ttctgcaggc accaggctgg ctcccaaacg ggcgccgcgt
      841 ctatgcggtc ggcgacatcc acggctgccg cgacaagctt atcgcgctgc acgggctgat
      901 cgccgcggat ttggcggcac gtccggccag gtccgcgctt ttggtgcatc tgggcgacta
      961 catcgaccat ggaccggcca gcgccggggt gatcgcgctg ctggcggcgg ctcctgtcat
     1021 cccgaacatc gcggtcgtca acctgctggg ggaccacgag cggatgttgc tcgatggcct
     1081 gggcggcgat cgggcagccg cgacggactg gctatgggcc ggcgggaagg cggcgctgga
     1141 aagctgggga ctaaccccgg acctgccgcg cgaggaatgg gaggcggccc tgcccgcttc
     1201 ccatatcgcg tggctgcgca gcctggtcct gacccatcgg gaaggcaact acctgttcgt
     1261 ccacgccggc atccggcctg gcgtcccatt gccggaccag tcgcgcgacg acctgctcac
     1321 gatgcgtcag ccgtttctgt caaccgaaca ggatctggga gtcgtcgtcg tgcacggcca
     1381 ttcgtcttca ccgtcggtat cgatcgcggc caatcggatc gggctcgaca ccggcgctgg
     1441 aagcggcggc aaactgacct gcgcgatcct ggaggatgac ctggtgggat tgctggcggt
     1501 gtagcaggat tgttggcggt gtagcaggat tgttggcggt gtagcgggat tgttggcggt
     1561 gtagcgggcg gatttcgtca gttgccgacc gcggcggcgg cccggatcgc cacggctttg
     1621 acgtcttcgc cgacaccatc ctcatacgcc gcgaaatttt cctcgaaacg ttcgatcagg
     1681 tgtttcgcca tatcgtcgta tgcggttttt tccgcccagg accggcgggg atccaggacc
     1741 tcgtcgggga tgccgggcac atgcgccggg atcgccaacc cgaaaaacgg ttccttgtgg
     1801 aactccatct gcgtcaacga accgtcgagc gcgcctcgca gcagcgcccg ggtgtggcgg
     1861 atcgacattc gcttgccgac gccgaacttg ccaccactcc agccggtgtt caccagccag
     1921 caggtggcac cgtgtttggc gatcaggtcg gcgaacatcc ggccgtaaac ctgcggcggg
     1981 cgcggcagga acggatgggc gaagcaagtg ctgaaggtgg ccttcggttc gcggccgagg
     2041 cccttttcgg ttccggcgac ctgcgcggta tagccggaca ggaagtggta catcgcctgc
     2101 gcgggtgtaa gtcgggagat cggcggcaac acgccgaacg cgtcgcaggt cagcatgaac
     2161 acgttcttcg gctgaccgcc gcggccgttc agatttacgt tcggaatgaa ctcgaccggg
     2221 tagcaggacc gggtattctc cgtcaggctg tcgtctgcga ggtccaggtt gccgtgccgg
     2281 tcgcccacga cgttctccag caccgcaccg aatcggttgg atgccgccca gatctcgggt
     2341 tcggcttccc gcgacaggtt tatcaccttg gcgtagcagc cgccttcgaa gttgaagatg
     2401 ccgttctcgc tccagccatg ctcgtcgtcg ccgatcaagg tgcgtgaggc atccgccgac
     2461 aaggtgg
//
LOCUS       DASWQN010000537         2790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_975375, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000537 DASWQN010000000
VERSION     DASWQN010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975375"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VGC82_08925"
     CDS             complement(<1..474)
                     /locus_tag="VGC82_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEY0423429.1"
                     /translation="MNTIDSPLFGLSQRLPPTNVQAEQALLGALLANNKAYERVSEFL
                     APEHFADPIHGRIFQAISRRVEAGQLADAVTLKAEFEHAGVLEEVGGTAYLSQLLTAM
                     VGIINAGDYGRAVLDTWLRRQLIDIGETVVNNAFGADAELDGPQQIEVAEQSLFNL"
     gene            complement(723..1295)
                     /gene="rplI"
                     /locus_tag="VGC82_08930"
     CDS             complement(723..1295)
                     /gene="rplI"
                     /locus_tag="VGC82_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY0423430.1"
                     /translation="MAAVELILLQRVENLGQMGDVVKVKPGYARNFLLPQRKALRASK
                     DNRARFDQQRAQLEAQNIRRREEAERLAERVGGLTVVIIRQAGEAGSLYGSVSARDIA
                     EAATAAGLTINRSQVVLETPIKTLGLSQVRVSLHPEVSIPVTANVARSPEEAERQARG
                     ERGAGQAEDEEEDRVPTAENIFEGDSPQPG"
     gene            complement(1308..1580)
                     /gene="rpsR"
                     /locus_tag="VGC82_08935"
     CDS             complement(1308..1580)
                     /gene="rpsR"
                     /locus_tag="VGC82_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY0423431.1"
                     /translation="MSDTEVNPASRRTAVGARRPFYRRRKSCPFSGPNAPTIDYKDVR
                     LLSRFLSERGKIVPSRITAVSAKRQRELAVAIKRARFLALLPYIVA"
     gene            complement(1587..2054)
                     /gene="rpsF"
                     /locus_tag="VGC82_08940"
     CDS             complement(1587..2054)
                     /gene="rpsF"
                     /locus_tag="VGC82_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY0423432.1"
                     /translation="MPLYETVLIARNDVTQQQVEQIADDIGTDLETDGGAVKKREYWG
                     LRGLAYRIKKNRKGHYMLLGIDAKPAFVTEMERKLGLNEDILRFMTIRTEAIDEAPSA
                     ILSRKSDERERGFRGPKPAGRFESGRRRGFDDREEFRARDAFGTEEFRPGTEE"
     gene            2688..>2790
                     /locus_tag="VGC82_08945"
     CDS             2688..>2790
                     /locus_tag="VGC82_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY0423433.1"
                     /translation="MTQFVSLYDAKTRLSALVDRASAGEEIVIAKNGV"
BASE COUNT          493 a          832 c          923 g          542 t
ORIGIN      
        1 aagattgaac agtgattgtt cggccacctc gatctgctgc ggaccgtcca gttcggcgtc
       61 ggcgccaaag gcgttgttga ccacggtttc gccgatgtcg atcaactggc ggcgcagcca
      121 ggtgtcgagg acggctcgcc catagtcgcc ggcgttgatg atgccgacca tcgcggtcag
      181 caattgggac aggtaagcgg tgccgccgac ttcctccagg accccggcgt gctcgaactc
      241 cgccttcagg gtgacggcat cggcgagttg ccccgcttcc acacgccggg aaatcgcctg
      301 gaagatgcgt ccatggatcg ggtccgcgaa gtgttcgggg gccaaaaact ccgagacgcg
      361 ttcatacgcc ttgttgttgg ccaggagagc gccgagcaag gcttgctccg cctggacatt
      421 ggtgggcggc aggcgttggg agaggccgaa gagtggcgag tcgatggtgt tcacgcagcc
      481 atcttagctc aggcgaatac gctgcgcatc ggctatgcct gtggacgaat gtggatagcg
      541 ggtgcaagtc gaggcgctgg ttctgtcgcc ttcggttcga ccgggcattg ggcgaacggc
      601 gattgggcga acggccgctg ccccgatcgc gtggcgtgac ggcgcgagtc cgtcgccgcg
      661 ccttccgcga tcaggccgtc gtgtggagga tcaccctgat ccgaactctc agggacaaat
      721 aatcagcccg gctgcggtga gtcgccttcg aagatatttt cagccgttgg aacgcgatct
      781 tcctcttcgt cttccgcttg accggcaccc cgttcgccgc gtgcctgccg ctcggcttct
      841 tccgggctgc gggcgacgtt ggcggtgacc gggattgaaa cctccggatg caacgacacg
      901 cgaacctgcg acaggccgag ggtcttgatc ggagtctcca acacgacctg cgagcggttg
      961 atggtcaggc ctgccgccgt cgcggcttcg gcgatgtcac gagcggacac cgaaccgtaa
     1021 aggctgcccg cttcgcctgc ctgacggatg atgaccacgg tcagaccgcc aacccgttcg
     1081 gcgagacgtt cggcttcctc acggcgcctg atattctgtg cttcgagttg cgcgcgctgc
     1141 tggtcgaacc tcgcgcggtt gtccttgctg gcgcgaagcg ccttcctctg cggcagaagg
     1201 aagttgcggg cgtaacccgg cttcaccttc accacatctc ccatctgccc caggttttcg
     1261 acgcgttgga gcaggatcag ttctacggcg gccatggaac cagctcctta ggcgacgata
     1321 tagggaagca gtgccaggaa ccgggcgcgc ttgatagcca ccgccagttc acgctgcctt
     1381 tttgccgaca cggcggtgat acgactcggg acgatcttgc cgcgttcgga cagaaaccgg
     1441 gacagcaggc gaacatcctt gtagtcgatc gtcggcgcgt tcggacccga aaacgggcag
     1501 gacttgcggc ggcgatagaa cggccggcgc gcgccgacag cggttcgccg ggaggcggga
     1561 ttgacttcgg tatcggacat tcggggctac tcctcggtcc cggggcggaa ttcttcggtg
     1621 ccgaaagcgt cgcgggcgcg aaattcctca cgatcgtcga aaccgcggcg gcggccgctt
     1681 tcgaagcgtc cggcgggctt gggcccgcgg aagccgcgtt cgcgctcgtc ggatttgcgc
     1741 gacaggatgg cggatggggc ctcgtcgatc gcctcggtgc ggatcgtcat gaaacgcagg
     1801 atgtcttcgt tgaggccgag ctttcgctcc atctccgtga cgaaggcggg cttggcgtcg
     1861 atgccgagca gcatgtagtg gcccttgcgg ttcttcttga tccggtaggc caggccgcgg
     1921 aggccccagt actcgcgctt cttgacggca ccgccatcgg tttccaggtc ggtgccgatg
     1981 tcgtcggcga tctgctcgac ctgctgttgc gtgacgtcat tccgcgcgat gagcacggtt
     2041 tcgtatagcg gcatggatca accccctatg gcttgtctct gccccggacc gggtctggcc
     2101 gagcttccgt gatggaagtg ggcatagccg ggcgcgatgc cgtcgcaccc ggcagggaag
     2161 cgcgggcttc aagcatggat tggcggggaa tgcaacggcg gaattggcac cgttccgaag
     2221 gtacagcgcc ggccacgatg ccggtgcgtc agccgttagc ccagcttccc gtattgtcct
     2281 gttgtagcgg cgccggggcg ttgtcggccg cctgagcgta aaaaggaggg gaaaacgtgg
     2341 caagaaccca tgccaggaat gccgttcgta tcaggatctt catcggattt ctctacaatc
     2401 gtcgggcgag acgtccggct cggtggaaca atcggacctg tacggccgga aaggcaattg
     2461 ggatccgcga catttatttt atgcacggga cgatgaaatc agaatgacgc caagcctcgg
     2521 aaaacagatg gtggcacatg gcgaccgcaa caaaccaatc agccggtcca tgagcggaga
     2581 ggcgagtgaa accgccccaa agtctgtgat ggcagccagt acgcatcgag tcattgactg
     2641 ttgccccgac gcatcgctat tggttggtca acctagtcaa ggggatcatg acccagttcg
     2701 tcagcctata cgatgcgaag acgcggctct ccgcgctcgt ggaccgcgct tcggctggcg
     2761 aggaaatcgt gatcgccaag aacggtgtcc
//
LOCUS       DASWQN010000538         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_447917, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000538 DASWQN010000000
VERSION     DASWQN010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447917"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VGC82_08950"
     CDS             complement(<1..606)
                     /locus_tag="VGC82_08950"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEY0423434.1"
                     /translation="MRRREFTALIGGAAAFPLVARAQQNREPRKVGILFPGVLGAERV
                     QWFTEGLTSQPGSEKAILVVRSAEGKDQLLGKYAGELAAEVDVILAVGSGSLFAARQV
                     SQTIPIVALDLESDPIATGAAQSLNRPGGNVTGIFFDAPEIAGKWIQIIREILPQIKK
                     VALLYDLHLDQTQLKSGETTARNAGIETLRFGIDQPAELRSA"
     gene            complement(828..1253)
                     /locus_tag="VGC82_08955"
     CDS             complement(828..1253)
                     /locus_tag="VGC82_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HEY0423435.1"
                     /translation="MATVLAGPRIGEHVACQRGQSKCVVKFAIAQLITNKPVTLTGYL
                     RFPVTAGLFRSNVEHAKRLWFTPDHLAMAQALGWDRVGSFYIDLEAPVLPSGIPKPGP
                     LQMHLFDDHMRYAIIWFSLAGLLLIAFAVWLRGRMPAPS"
     gene            1170..1398
                     /locus_tag="VGC82_08960"
                     /pseudo
     CDS             1170..1398
                     /locus_tag="VGC82_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1922..>2573)
                     /locus_tag="VGC82_08965"
     CDS             complement(1922..>2573)
                     /locus_tag="VGC82_08965"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEY0423436.1"
                     /translation="RIHGELLKLGVDVGQTTVAKYMTRGRRPPFQGWKTFLHNHADGI
                     ASMDLFVVPTISFRLLYGLLILHHDRRQILGLGVTAHPTAEWISRQLTEACGWKVTPR
                     YIIRDRDAVYGDVFIRRLRAMSIRDRPTAPRSPWQNGYCERAIGSIRRECLDHVVVFG
                     KRHLRHLLRSYATYYNSARTHLSVNKDAPSPRTVHAVGRIVPTPFLGGLHHLYVRV"
BASE COUNT          539 a          729 c          774 g          531 t
ORIGIN      
        1 cgcgctgcgc aattcggcgg gttgatcaat gccgaaccgc agtgtttcaa ttccggcatt
       61 gcgggcggtg gtttcgccgg atttgagctg cgtctggtcg aggtgcaagt cgtaaagcag
      121 cgcaaccttt ttaatttgag ggagtatctc cctgataatc tgaatccatt tgcccgcaat
      181 ttctggtgca tcgaagaaga tacccgtcac attgccgccg ggccgattga ggctttgcgc
      241 tgctccggtc gcaatgggat cagattccag atcaagcgcg acaatgggaa tggtttgtga
      301 cacctgccgc gcggcgaaaa gactccccga tccaacggca aggatgacgt ctacctcagc
      361 cgccagttcg cccgcgtatt tgccaagcaa ttggtctttg ccttcagcgg atcgcacgac
      421 caagattgct ttttcgctgc cgggttggct ggtcaatccc tcggtgaacc attgcactcg
      481 ttccgcaccc agcacacccg gaaaaaggat gccgactttt cgcggctccc tattctgttg
      541 cgcgcgcgcg acgagcggaa acgccgcggc acctccaata agggcagtga actcacgccg
      601 tcgcatatga tgccaccgag agcctgcttg cggaacccaa ctatagcagt ttgacccatt
      661 cagcgtgcga caattagtga ctacagcccc aatgctcatc aggctcatgt ccgttcgggg
      721 tcattttcag acttgggggc gcgcagttgc gaggtccgct ttaccccgat gagccgacct
      781 cgtcagctta atcgctttcg ttcgaaaagt gccaccagaa gccgacatca ggaaggggca
      841 ggcatacggc cgcgcagcca gacggcaaag gcgatcagca gcaagcctgc cagcgaaaac
      901 cagataatcg catatcgcat gtgatcgtcg aacagatgca tctgaagcgg cccgggtttc
      961 gggatgccgc tcggaagcac tggagcctcc agatcgatgt agaacgaccc aacccgatcc
     1021 cacccgagcg cctgcgccat cgcgagatga tccggggtga accacaaccg cttcgcgtgc
     1081 tctacgttgg acctgaacaa gccggctgtc accggaaaac gcaggtagcc ggtcagcgtg
     1141 acgggcttgt tggtgatgag ctgcgctatc gcgaacttga cgacgcactt ggattgaccg
     1201 cgctggcagg cgacgtgctc gccgatgcgc ggaccggcaa gaacggtcgc catgctgtgg
     1261 caatcggggt tcggccgtct cgctggctac gagagtgtga acgatgccga acgtcttcgt
     1321 cacgatcctg caatgcgctg gatcgtcggc ggcaaggcgt cccagggccg cgcggcttcg
     1381 ccaagccaga tgggccgcta gtcttcataa aatgagacat tgcggatcag gcgcctttga
     1441 tgtccgatat accctcaaca gcggcgcgaa gtcggtccgg atgtccgagt tggatctagg
     1501 ctgtgtgtgg caaggaggcc aaggcatgag gataatgacc gtagcgttcg ctctcggaac
     1561 ggccctggtg ctgtgtaacc tggggacgtt tagtgtcgca gcccatgcgc gggatgccaa
     1621 agttgttcgt gaaccacggg tgaggcttat cgtcagggtg cccgtggaaa ggccttactg
     1681 ggattcttat attgttccac gatatcgtta tcgccctgag gacgaccggg tcgatccatg
     1741 cggcgggccc gtagtgccgg taataagagt tgggcctcaa gaggaaactg ttccgttgtg
     1801 gctggcgaac gctttgggtc ttggccgtgt gcatggtcga gattggccgt gtaagggtca
     1861 aagccggtaa tgcaccgatt gagcagaaaa tttcagctta tccccctgtg cctgtcggaa
     1921 atcaaactcg cacatacaga tgatgaagtc cgccaagaaa tggcgtaggc acaatgcgac
     1981 caacggcatg taccgttcgc ggcgacggcg cgtccttgtt gactgaaagg tgcgtgcgag
     2041 ccgagttata ataggtcgcg taagatcgca gcagatggcg aagatgccgc ttgccgaaca
     2101 cgacaacgtg gtcaagacat tcccgccgga tcgaaccgat tgccctttcg caatatccgt
     2161 tctgccacgg cgatcgcggc gcggttggcc gatcccgaat gctcatcgcc cgaaggcggc
     2221 ggatgaagac gtcaccgtag accgcgtcgc ggtcacgaat gatgtatcgc ggcgttactt
     2281 tccagccaca ggcctcggtg agctggcgcg aaatccattc cgccgttggg tgcgcggtca
     2341 ctcccagccc caggatctgg cggcgatcgt gatggagtat cagcaacccg tagagcagtc
     2401 ggaatgagat tgtcggaaca acgaataggt ccatcgacgc gatgccgtcg gcgtgattat
     2461 gaaggaacgt cttccaccct tggaacggcg gtcgccttcc cctcgtcata tactttgcaa
     2521 cggtggtttg cccgacatcg acgccgagct tgagaagctc gccatggatc cgt
//
LOCUS       DASWQN010000539        14289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2031423, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000539 DASWQN010000000
VERSION     DASWQN010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 14289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14289
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2031423"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VGC82_08970"
     CDS             <1..478
                     /locus_tag="VGC82_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423437.1"
                     /translation="AFVVTRDAAGSNDRRGAYAHVNPPVFDGAWQWRDADGNQQPRVS
                     LFAARYSQSNPILPDLDKTYRSFALRIRDESCLNCHTPINRAESERLVLLQTPQHAAG
                     EIDNVIKAVKSGEMPQDDIGLRKDVPAERRAAILDAALSFRDELAMADQWEAGHTR"
     gene            585..2996
                     /locus_tag="VGC82_08975"
     CDS             585..2996
                     /locus_tag="VGC82_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0423438.1"
                     /translation="MITRDSAHQAGEPRRSAVTPRSTLARLGFVLRLHHLLFIAFTII
                     AATPIAVLAWWEGNTSFQNELDSVRERHLLVARNLTSTMSRYVKDVEAVFGLAFESGA
                     LNRPVAGLTDLLTSLNVIHIFCIAPDGGVEARLPGLSADPADQLPSPVVSDLRLLAAN
                     AHGQIAISNLYHDAAGLPVFYLLKQLPGGRLGVGVMSTTYLVSLQQAIAFGDHGHAVM
                     VDAKGQVIAHPFKDWVAASRDISGVPAVAAMMRGETGVGQFYSPAFSDNMIAGYAVVP
                     ETGWGVMVPQPITELRRRASQVNEMATVIALISFGGAALMAWLLALYLARPVRIVAAT
                     AEAVLGGNDEVAVPEFRGLVPHEIRRLGAGFNTMLDGLRQREAETTQALRQAETSNAA
                     KSQFLANMSHEIRTPLNGVVGMIELLQLTDLSSPQRRYLETATQSSQSLLRLIDDILD
                     LSRIEVGRLELEHAPFHLPSLIHDLRVLFADQARSKGLTLHMSIPDALNLMLVGDQHR
                     LLQILTNLISNALKFTSDGSITLRGMVEQKVGTGLRIRFEVSDTGIGIPANKQAMIFE
                     AFSQADSSTTRRYGGTGLGLSIARQLCHMMDGDIGVESVAGIGSTFWFTVMIEQGEAV
                     APAPVRPAAASVPPPAKAINALTPEPMRLVPFVSRTGRDFQAVLEQGGRGQVSILLVE
                     DNPANMRVTQALLESLGCRVTPAVNGLDAVGTYREAEFDLVLMDCQMPEMDGYEATRT
                     IRQIEGFHGRRTPIIALTAHAMEGSREISLNSGMDDQITKPLTMAALTGKLLEWLVPA
                     EAQAV"
     gene            complement(3149..4213)
                     /locus_tag="VGC82_08980"
     CDS             complement(3149..4213)
                     /locus_tag="VGC82_08980"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEY0423439.1"
                     /translation="MMKPLPSEPFGQMPDGRDTTLYTLENDHLRVRITDFGGRMVSIE
                     APDSRGQREHVLLGFDNLAAYRANGGSFGTLLGRYANRIAGASFTLDGQSFQLSRNNG
                     DATLHGGASGFDKSVWSVAAAEAEPIPTLVLTHRSPDGDQGFPGEVSVQATYQLGSDT
                     LSLTYEARTTKPTVINLSVHPYFNMGGTAAGDVLKHEVTIVAEAFLPTDAAQIPTGEI
                     RPVAGTPFDFRQKMPLGARIRRADPQLFHGLGYDHCFVLGSEVAAEPSLAVRVRDPRS
                     GRVLEVHTDQPGVQVYTANKLTGAFAGHGGIIYRQSSGLALEPQGFPDAPHHPNFPSS
                     VLRPGEIYRRTIRYRFLAGA"
     gene            4355..5107
                     /locus_tag="VGC82_08985"
     CDS             4355..5107
                     /locus_tag="VGC82_08985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0423440.1"
                     /translation="MLRLEAKVAVVTGAGTGIGRATAKLFAREGAKVVVAELNAEAGE
                     QTAQLIVQAGGDAIAIRTDVTEPDSIQATIEKAEQHYGALHVLHNNAGGSTSRDGDVT
                     AAPLEEFWRVIKLDLFGTFLGCRFGIPAIIRSGGGSVINMSSNVALMGIPGRDCYTAA
                     KGAVAAMTRSMAVEFAPKVRVNAIAPSVTMTDRVVKLVAGNAALDKLADSHLLGLIEP
                     EDIAAMALFLASDESRKVTGQIYPVDSGVTIS"
     gene            5126..5539
                     /locus_tag="VGC82_08990"
     CDS             5126..5539
                     /locus_tag="VGC82_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0423441.1"
                     /translation="MKVKRVEHIAIAVDSMQQSLALMRDTFGLELEYEEQIGQTRLAM
                     LPVGQSYIELLQGQGPDSGVTKWIAEKGAGLFHICFEVDDIEGAIAELKAKNVKLQSD
                     TWRTGHAGSKIVFLDPSATGNVVIELAELAAGHQS"
     gene            5536..6402
                     /locus_tag="VGC82_08995"
     CDS             5536..6402
                     /locus_tag="VGC82_08995"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY0423442.1"
                     /translation="MTGFLHPEFLVETEWLAEHLDNVVILDCTVHLIPDPKTTYVVQP
                     GREDYEKAHIPGSLFCDVSKDVSDTRQKLNFMRPSPEDFAAAMRRFGIRNSRRVVTYS
                     TANPWWATRVWWLLREFGHDNAAVLNGGWQKWVAENRAVETGSPRQRPAGDFTVQKIR
                     NLMVGKNEVKAAIGDTGICTLNALLPAQHTGSGGNSYGRPGRIAGSVNLPAAHLLDPA
                     TNTFLPADELRRRLGSVGALDRPVIAYCGGGIAASADAFVLTMLGHNDVRIYDASLSE
                     WAKDESLPMETG"
     gene            6462..7241
                     /locus_tag="VGC82_09000"
     CDS             6462..7241
                     /locus_tag="VGC82_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0423443.1"
                     /translation="MRLENKIGIVTAAGSGMGRAGATRFAAEGASVAVVDLDKAATDA
                     VVRQIEAAGGKAIGLSGDLTNDDFARDIVRQTVRHYGGLDFVWNHVGTPGPASIEGLD
                     WKDFDFAMNLNVRTVLVTTETALPELRARGGGSLLFTASTSGLQGSPYSPVYNIAKFG
                     VVGLVKGLAKRYGHENIRVNAVCPGTTDTPMLRVFVARPDSQLPPGETPETLVAKRGG
                     QNPMRRVAQPQEIANAALFLLSDEASFVNGVALPVDGGATA"
     gene            complement(7267..7617)
                     /locus_tag="VGC82_09005"
     CDS             complement(7267..7617)
                     /locus_tag="VGC82_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0423444.1"
                     /translation="MSKIIRTEPGPILSKAVEYHGFVYLPGITAKDTSKDIKGQTAEV
                     LEQIDAMLEQHGTDKTRLLQATIWVKDIKERGMMNEVWTPWLPKEGAPARACVQAEMA
                     SPNVRVEIMVTACK"
     gene            7749..8729
                     /locus_tag="VGC82_09010"
     CDS             7749..8729
                     /locus_tag="VGC82_09010"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY0423445.1"
                     /translation="MKICIYGAGAIGGHLAARLYRAGADVSVIARGAHLAAIQANGLT
                     VHAIDGEHHAPVRASDDPAELGRQDAVFVTVKAPSLPAVAASISPLLGTDTPVAFVMN
                     GIPWWYFDHLAGPHEGKSLPRIDPNDALRHALGQGRTIGGVVYSASAVTAPGVVEVEQ
                     PKSRIILGEPDGSLSDRVLTLSGLITKGGISGEATPAIRTEIWNKLISNLAGGSLAVL
                     TGSAPKGIYTTEPAAEQAALRMMQEATAIAQALGADPTTDHTRRVTGQRSMDHKPSIL
                     QDLELGRPMEIDGLFDAPLALAHLAGVEVPTLELMVALCKLRARSSGLYN"
     gene            8839..9837
                     /locus_tag="VGC82_09015"
     CDS             8839..9837
                     /locus_tag="VGC82_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0423446.1"
                     /translation="METRIFGRSGMRLSILGFGCGGVGGQMVRGSAADQDRAIGMALN
                     AGINYFDTAVQYGDGASETNLGRTLAGRKTSDIAVGTKVRIPAAKFAAIGQTIADSMD
                     GSLGRLGMDHVDIFHLHNAITLNGGGESLSVAQVLHEVVPAFEALRKAGKTRFLGITA
                     VGETAALHQVIDAEVIDSAQVSYNMLNASAATALPANYPAQDYGRLFDHTQKAGVGVV
                     GIRVLAAGALSGTSERHPTAGAPPEPIGSAHSYEMDLERARRLMPVVTEGFASSLAEA
                     ATRFAITHPAMGTILVGMATVEQFQASLDAVLKGPLPQAALDRLSVLTAGFAGEPR"
     gene            complement(10045..10806)
                     /locus_tag="VGC82_09020"
     CDS             complement(10045..10806)
                     /locus_tag="VGC82_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0423447.1"
                     /translation="MQLKDLAALVTGGGSGLGAATAAHLAALGCKVAVADINQAAAES
                     VAASINGIGVRCDVADAASGEAAFAAARAAHGKVRILVNCAGIGTAGRIVGRDGPMKL
                     EAFERVIRVNLIGSFNMLRLAAAEMSAAEALEEGERGIIVNTASIAAFEGQVGQPAYA
                     ASKGGVVAMTLPAARELARAGVRVVTIAPGLFQTPMVDGLPPEVQASLGAGVPFPPRL
                     GRPSEFAELVEHIVRNRFLNGEVIRLDGAFRMPPR"
     gene            10954..12840
                     /locus_tag="VGC82_09025"
     CDS             10954..12840
                     /locus_tag="VGC82_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0423448.1"
                     /translation="MTTAPTYADAYEDWARDASAYWAREAQGITWPKTWDKVFEPGLG
                     AFGQWFAGGMLNTCYNCLDRHVEAGRGEQAALIHDSPMTDSVRTFTYRDLLERTARLA
                     GALSALGVTKGDRVVIYMPMVPEAAIAMLAVARLGAVHSVVFGGFAAAELSTRIADAK
                     PKVIVSASCGLEPGRVVQYKPLLDAAIALSPHKPDACLILHRPQAGAPMTPGRGHDFN
                     AAVDAAEPHGCVAVLATDPLYILYTSGTTGRPKGVVRDNGGHAVVLWNSMKMIYGLDE
                     GDVFWTASDVGWVVGHSYIVYAPLLRGCTSVMYEGKPVGTPDAGAFWRVIAQHRVKVL
                     FTAPTAMRAIKQQDPEGRLARSHDLSSLEALYLAGERCDPPTAVWAHELLGKPVVDHW
                     WQTETGWPITAGFRQFGLFPFKPGSGGRPCPGYDLHALDDDGKVLPAGETGNLSLRLP
                     LPPGCGPTLWQNDEGYRASYLADFPGWYRTGDAGMVDTDGDVWVMGRTDDIINVAGHR
                     LSTGSMEEVLASHGDVAECAVIGAKDELKGQTPIGFVVLKAGVDRPEAEVAAELVALV
                     RERIGPVASFRDARVVARLPKTRSGKILRASIRKIADGEEAAVPPTIEDPAALDELRL
                     VLQR"
     gene            13089..13178
                     /gene="kdpF"
                     /locus_tag="VGC82_09030"
     CDS             13089..13178
                     /gene="kdpF"
                     /locus_tag="VGC82_09030"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEY0423449.1"
                     /translation="MILDYALGGIVTVFLLAYLVVALIKPERF"
     gene            13311..>14289
                     /gene="kdpA"
                     /locus_tag="VGC82_09035"
     CDS             13311..>14289
                     /gene="kdpA"
                     /locus_tag="VGC82_09035"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY0423450.1"
                     /translation="MTVNGWLQVALFCVLLVLLTKPLGGYMTRVFAGDKTLLSPVLRP
                     LETFLYRLSGIDERLEQHWVTYAVAMLAFSFAGFVVLYALQRLQAILPLNPQHLDAVS
                     PDLAFNTSVSFVTNTNWQSYVPETTLSYLVQMAGLTVHNFVSAATGIALAIALVRGFT
                     RRSSATVGNFWVDMTRCTLYVLLPASIVFGLVMVSQGVPQNLSAYTDVTTLEGAKQVI
                     AQGPVASQEVIKMLGTNGGGFFNANSAHPFENPTALTNLLQMLLIFSIGAALTNVFGR
                     MAGNQRQGWAIFAVMGVMFLAGVTVLYASEAAGKALLAHLPLDQLAGNME"
BASE COUNT         2676 a         4551 c         4445 g         2617 t
ORIGIN      
        1 ggcgttcgtc gtgacgcgcg atgccgccgg cagcaacgac cgccgcgggg cctatgccca
       61 cgtcaacccc ccggtcttcg atggcgcctg gcagtggcgc gatgccgacg gcaaccagca
      121 gccccgggtc tcattgttcg ccgcgcgcta cagtcagtcc aacccaattc tgcccgacct
      181 ggacaagacc tatcgttcgt ttgcgctgcg catccgcgac gagtcatgcc tgaactgcca
      241 taccccaatt aaccgagcgg agtcggaacg ccttgtgctg ctgcagacgc cgcaacatgc
      301 cgccggcgag atcgacaatg tcatcaaggc agtcaaaagc ggggaaatgc cgcaggacga
      361 tattgggctg cgcaaggatg tcccggccga acgtcgcgcc gcgatactgg atgccgctct
      421 ttcattccgt gatgaactgg caatggcaga ccagtgggaa gccggtcaca cacgctgacc
      481 aggcgacccg aatattcatt caataagata tttattgtat agtcaaacag actatggcct
      541 ataagataaa ggatgctcag aatgcgacga atggcagcgg tcaaatgatc acgcgcgact
      601 cggcccatca agctggcgaa ccgcggcggt cagccgtcac gccgcgttcg actttggcgc
      661 ggttgggctt cgtcctgcgc ctgcatcatc tgctgttcat tgcattcacc atcattgccg
      721 caactccgat tgctgtcctt gcttggtggg aaggcaatac atcgtttcag aacgaactcg
      781 actccgttcg tgagcggcat ctgctggtcg cccgcaacct gacatccacc atgtcgcgct
      841 acgtcaagga tgtggaagcg gtcttcgggc tggccttcga aagcggtgcg ctcaaccgcc
      901 cggtcgcggg actaacggac ctgctgacat cgctgaacgt catccatatt ttctgcatcg
      961 ctccggacgg tggcgtcgaa gcgcgattgc ctggcttgtc cgccgatccc gccgatcaac
     1021 tgccgtcgcc ggtggtatcg gacctgcggc ttctggctgc caacgcgcac ggccagatcg
     1081 cgatcagcaa cctgtatcac gatgccgccg gcctaccggt tttctatttg ctcaagcagc
     1141 ttccgggtgg gcggcttggc gtcggcgtca tgagcaccac ctatcttgtc tcccttcagc
     1201 aggccatcgc gttcggcgat catggccatg ccgtcatggt ggacgccaag ggtcaggtca
     1261 tcgctcatcc cttcaaggac tgggtggcgg cgtcccgcga tatttccggc gtgccggcag
     1321 tggcggcgat gatgcgcggc gaaaccggag tcggtcagtt ctactcgccc gcgttcagcg
     1381 acaacatgat cgccggttac gcggtggtgc ccgaaaccgg ctggggcgtg atggtgccgc
     1441 agccgatcac cgagctccgc cggcgcgcta gtcaggtgaa cgagatggcg acggtgattg
     1501 ccctgatatc gttcggcggc gccgcgctta tggcctggtt gctggcgctt tacctcgctc
     1561 ggccggtgcg catcgtggcg gcaacggccg aggctgttct cggcggcaat gacgaagtgg
     1621 cggtgccgga atttcgtggc ctggtgccgc acgaaatccg ccgcttgggt gccggtttca
     1681 acaccatgct ggatggcctg cgccagcggg aggccgaaac cacgcaggcg ttgcgccagg
     1741 cggaaacctc caacgccgcc aaaagccagt tcctggcgaa tatgagccat gaaatccgca
     1801 cgccgttgaa cggcgtggta ggcatgatcg agcttttgca actgacggac ctgtcgtcgc
     1861 cacaacggcg ctatctcgaa acggcgaccc agtcgagcca gtcgctgctg cggttgatcg
     1921 acgacatcct cgatctgtcg cgtatcgagg tcggccgtct ggagctggag catgcaccgt
     1981 tccacctccc ctcgctcatc cacgaccttc gtgtcctgtt cgccgaccag gctcggtcca
     2041 agggtctgac gctgcacatg tcgattccgg atgcattgaa cttgatgctg gtgggcgacc
     2101 agcaccgtct gctgcagata ttgaccaatt tgatcagcaa tgccctgaaa ttcacttcgg
     2161 atggcagcat caccttgcgg ggcatggtgg agcagaaggt tggtaccggt ttgcgcatcc
     2221 gcttcgaggt ctccgacacc ggcatcggca ttccagcaaa caagcaggcg atgattttcg
     2281 aagcgttcag ccaagccgac agttcgacca cccgccgcta tggcggcacc ggcctcggtt
     2341 tatcaatcgc gcgccagctt tgccacatga tggacggcga tatcggcgtc gaaagcgtcg
     2401 ctgggattgg gtcgaccttc tggttcacgg tcatgatcga acagggagag gcggtagccc
     2461 cagctccagt acggccggcg gctgcttccg tgcctccgcc cgccaaggcg atcaacgccc
     2521 tgacacccga accgatgcgg ctcgtcccgt ttgtttcccg gacggggcgc gatttccagg
     2581 ccgtgctgga acagggcggc cgaggccagg tgtcgatcct gctggtggag gacaacccgg
     2641 ccaacatgcg cgtaacgcag gcgttgcttg aatcgctggg atgtcgcgtc acacccgcgg
     2701 tcaatggact ggatgcggtc ggaacctacc gcgaggctga attcgacctg gtgctgatgg
     2761 actgccagat gccggaaatg gacggttatg aggccactcg gacaatccgt cagatcgaag
     2821 ggtttcatgg ccggcgcaca ccgatcatcg cgctgacggc ccatgcgatg gaaggcagcc
     2881 gggagataag cctgaatagc ggcatggacg accagatcac caagccgctg acgatggcgg
     2941 cgttgaccgg caagttgctg gagtggctgg tgccggcgga ggcgcaagca gtttagccag
     3001 gctcgatttt tctgcacacg aaacaaaaat cttccagcta aagcatcttg tttgatcgca
     3061 tgattcacac gcttcggacg ttccgcccag cgtgatcatc ttgtttgatc gcatgattca
     3121 cacgctttgg acgttccgcc cagcgtgatc atgccccagc gagaaaacga taccggatgg
     3181 tccggcgata gatttccccc ggccgcagaa ccgaagacgg gaagttcggg tggtgcggcg
     3241 catccggaaa gccctgcggt tccagcgcaa ggcccgatga ctggcgatag ataatcccgc
     3301 catgtccggc aaaggcgcca gtcagtttat tcgccgtgta aacctgcacg cccggctgat
     3361 cggtatggac ctccagcacg cgaccgcttc tcggatcgcg cacccgcacc gcgaggctgg
     3421 gttcggcggc aacctcgctc cccagcacaa agcaatggtc gtagcccagg ccgtgaaaca
     3481 actgcggatc cgcgcgcctg atccgcgcac ccaatggcat cttctggcgg aagtcgaaag
     3541 gcgtgccggc cacgggacga atttcgccgg tgggaatctg cgcggcgtcg gtcggcagga
     3601 atgcttcggc cacgatggtg acttcgtgct tcagcacatc gcctgcggcc gtgccgccca
     3661 tgttgaaata cggatgcacg ctcaggttga taacggtcgg cttggtcgtc cgcgcctcgt
     3721 atgtgagtga caaagtgtca gaaccgagct gataggtcgc ctgaacggaa acctcaccgg
     3781 gaaagccttg atcgccgtcc ggactccgat gggtcagcac cagcgtcggg atgggttcgg
     3841 cttcggcggc cgccacggac cataccgatt tatcgaaccc ggatgctccg ccgtgcaagg
     3901 tagcgtcgcc gttgttcctg gaaagctgaa agctctggcc atccaaagtg aagctggcgc
     3961 ctgctatgcg attggcgtag cgcccgagca gagtgccgaa agagccgccg ttggcgcggt
     4021 aagcggcgag gttgtcaaag cccagtaaca catgttcgcg ctggccacga ctgtctggcg
     4081 cctcgatgct caccatacgg ccgccgaaat cggtgatccg gaccctcaaa tggtcgtttt
     4141 ccagggtgta caacgtcgtg tcgcgcccat ccggcatctg gccaaacggt tctgagggta
     4201 aaggtttcat catctgattt cctgcgcggt gccaagctga tcccgtctat cacagaccgg
     4261 gatgacgccg aacggacttc cacaggaaca gcctcccctt gatccggtgc ggcgaccggg
     4321 cgacgatgac tcccaatcaa cacgaggaac gtcgatgcta cgcctggaag caaaggtcgc
     4381 tgtcgttacc ggcgctggaa ccgggatcgg acgagcgacc gccaagttgt tcgccaggga
     4441 aggtgccaag gtcgtcgtcg cggaactgaa cgccgaggcc ggcgagcaga ccgcgcaact
     4501 gatcgtccag gccggcggcg atgctatcgc gatcagaacg gatgtcaccg aaccggacag
     4561 cattcaggca accatcgaaa aagccgaaca gcattacgga gcgctgcacg tcctgcacaa
     4621 caatgccggc ggctccactt cgcgtgacgg cgatgtcacc gccgcaccgt tggaagaatt
     4681 ctggcgcgtc atcaagctgg acctgttcgg cacgttcctc ggctgtcgct tcggtatccc
     4741 ggcgatcatc aggtccggcg gcggttcggt catcaacatg tcgtcgaacg tcgcgttgat
     4801 ggggatcccg gggcgggatt gctatacggc tgccaaaggc gcggtcgctg caatgacccg
     4861 atccatggcg gtggagttcg cgccgaaagt ccgcgtcaac gccatcgccc catccgtcac
     4921 gatgacggac cgagttgtga aactagttgc cggcaatgcc gcgctggaca aactcgcgga
     4981 ctcccatctg ctcggtttga tcgaaccgga ggacatcgcc gcgatggcgt tgttcctggc
     5041 ttcggacgaa agccggaaag taaccggcca gatctacccg gtggacagcg gcgtcacgat
     5101 ttcctgaacc aacggagaag taacgatgaa agtcaagcgc gtcgaacaca tcgccatcgc
     5161 cgtcgactcc atgcaacagt cgctggccct gatgcgcgac accttcggtc tggaactgga
     5221 atacgaagaa caaattggcc agaccaggct ggcgatgctg ccggtcggcc aaagctacat
     5281 cgaactgctg caggggcagg gacctgactc cggcgtgacg aaatggatcg ccgagaaggg
     5341 cgccggactg ttccatatct gttttgaagt cgatgatatc gaaggcgcta ttgcggaact
     5401 gaaggccaag aacgtgaaac tgcaaagcga cacctggcgg actggccatg ccggctcaaa
     5461 gatcgtcttc ctggatccct cggcgaccgg gaatgtggtg atcgaactgg ccgaactcgc
     5521 agcggggcat cagtcatgac aggtttcctg catccggagt tcctggtcga gacggaatgg
     5581 ctcgccgaac acctggacaa tgtggttata ctggattgca cagtccacct cattcccgat
     5641 ccgaagacaa cctatgtggt gcagccgggg cgggaggatt acgagaaagc ccatatcccc
     5701 ggctcgttgt tctgcgacgt gtcgaaggat gtgtcggaca cacggcaaaa actaaacttc
     5761 atgcgcccgt cgccggaaga ctttgccgcc gcgatgcgtc gtttcggcat cagaaattcc
     5821 aggcgcgtcg taacctacag caccgccaat ccgtggtggg caacccgggt ctggtggctg
     5881 ctgcgcgagt tcggtcacga caacgcggcg gtgctgaacg gcggctggca aaaatgggtc
     5941 gcggaaaacc gggcggtaga gaccggcagc ccccgccaac gtccggcggg cgacttcacc
     6001 gtgcagaaga ttcgcaacct gatggtcggc aagaacgagg taaaggcagc tattggcgac
     6061 accggcatct gcacgctgaa cgccctgctg ccggcgcagc ataccggcag cggcggcaac
     6121 agctacggcc gccccggccg catcgccggc agcgtcaatc taccggccgc acatctgctc
     6181 gatcccgcca caaacacgtt cctgccggca gacgagttgc gccgccgcct gggctccgtg
     6241 ggggcactgg atcgtccggt aatcgcatat tgcggtggcg gcatcgcagc cagcgccgac
     6301 gccttcgtcc tcaccatgct cgggcataat gacgtgcgta tatacgacgc atcgctgtcg
     6361 gaatgggcga aggatgaaag cctgcccatg gaaacgggat aaggcaggac gaaaacgccc
     6421 gcgatcatcc cgcaggctaa cccgcaaacg aaggaacaac catgcgcctg gaaaacaaaa
     6481 tcggcatcgt gaccgcggct ggctccggca tgggccgagc cggggcgacg cgttttgctg
     6541 ctgaaggcgc ctcggtcgcc gtcgtggatt tggacaaggc agcgaccgac gcggtcgtac
     6601 ggcagatcga ggcagccggc ggcaaggcca tcggcctctc cggcgacctg accaacgacg
     6661 actttgctcg cgatatcgtg cggcaaacgg taaggcatta cggcggcctc gatttcgtct
     6721 ggaaccatgt cggcacccca ggtccggcct cgatcgaggg gctcgactgg aaggacttcg
     6781 acttcgcgat gaacctcaat gtccgcacgg tgctggtgac caccgagacg gcgctgccgg
     6841 aactccgagc ccgcggcggt ggctccttgc tgtttaccgc ttctacgtcc ggtctgcagg
     6901 gatcgccgta cagcccggtt tacaatatcg ccaaatttgg cgtggtcgga ctggtgaagg
     6961 gactggcgaa gcgctacgga cacgaaaata tccgggtcaa tgccgtctgc cccggcacga
     7021 ccgatacgcc gatgctgcgg gtcttcgttg ctcggccgga cagccagttg ccgcctggcg
     7081 agacgccgga aaccctggtc gccaaacggg gtggccagaa cccgatgcgg cgggtcgctc
     7141 aaccgcagga aatcgccaac gcggcgctgt tcctgctgtc ggatgaggcg tcgttcgtca
     7201 acggcgtggc gctgccggtg gatggcggcg ccacggccta ggccacatcc gcgcgcagga
     7261 cgtcggttac ttgcaggcgg tgaccatgat ctccacccgc acgttgggcg aggccatctc
     7321 ggcctgaacg caggcccggg ccggggcgcc ttcctttggc agccacgggg tccagacctc
     7381 gttcatcatg ccgcgctcct tgatgtcctt cacccagatc gtggcctgca gcaggcgggt
     7441 cttgtccgtg ccgtgttgtt ccagcatcgc gtcgatctgc tccagcactt cggccgtctg
     7501 gcctttgatg tccttgctgg tgtccttggc agtgatgccg ggcagataga caaagccatg
     7561 gtattcaact gccttggaga gaatcgggcc aggttcggta cggatgatct tgctcatgga
     7621 acgctccagg ttaggacgga tctcagggtt ctaaggcaag ccgccgccgc cgaccagagc
     7681 gccgcgggtc ccggacttga cccgatccgc agtcctggcg tcggatatgg caaccagcaa
     7741 ggatcgtcat gaaaatctgt atctacggcg ccggcgcgat cggcggtcat ctcgcggctc
     7801 ggctttatcg ggctggggcc gacgtatccg tcatcgcccg cggcgcccac ctcgcggcaa
     7861 tccaggccaa tggcctgacg gtccatgcca tcgatgggga gcaccacgcg ccggtgcggg
     7921 cgtcggacga cccggcggaa ctcggcaggc aggatgctgt gttcgttacc gtcaaagccc
     7981 cttcgttgcc ggccgtggca gcgtccatct cgccgctgct cggcaccgac acgccggttg
     8041 ctttcgtcat gaacggcatt ccgtggtggt acttcgatca cctggccggt ccgcatgaag
     8101 ggaaatcgct gccacgcatc gaccccaacg acgctctgcg ccatgcgctc gggcagggcc
     8161 ggacaatcgg cggcgtggtc tattccgcga gcgccgtcac cgcgcccggg gtggtcgaag
     8221 tggagcagcc gaaaagccgc atcatcctag gcgaaccgga cggctcgttg tcggaccggg
     8281 tcctgacgct gagcggcctg atcaccaagg gcggcatcag cggcgaggcc acgccggcca
     8341 tccggacgga gatctggaac aagctgatca gcaacctggc cggcggcagt ttggcggtac
     8401 tgaccggcag cgcgccgaaa gggatctata ccaccgaacc cgcggccgaa caggccgcct
     8461 tacggatgat gcaggaggcg acggcaatcg ctcaagcgct gggcgccgac ccgacaaccg
     8521 accacacacg acgcgttacc gggcaacgct ccatggatca taaacccagc atcctacagg
     8581 atctggagct tggccggccg atggagatag acggtctgtt cgacgcgccc ctggcgctgg
     8641 cgcatctggc cggcgtcgag gttccgacgc tggagctgat ggtggcgctc tgcaagctgc
     8701 gggcccgatc gtcggggctc tacaactgac cagtgtcctg cctccgaagt tcgccacatt
     8761 ttgcgacgga cgccgaacat cagcatgcca ctgaaaacaa ctggcaccaa cgaacgcgga
     8821 aaccgaggga accctaccat ggaaacacga attttcggcc gctccggcat gcggctttcg
     8881 atattgggct tcggctgtgg cggcgtcggc ggccagatgg tgcgcggttc ggccgccgac
     8941 caggaccggg cgatcgggat ggcgctgaac gccgggatca actacttcga taccgccgtc
     9001 cagtacggcg atggcgcgtc cgaaacgaac ctgggacgca ccctggcggg gcggaaaacc
     9061 tctgacattg ccgttggcac gaaggtccgc attcccgcgg cgaagttcgc ggccatcggc
     9121 cagacgatag ccgattccat ggacggcagc ctgggccgac ttggcatgga tcatgtcgat
     9181 atctttcact tgcacaatgc catcacactg aacggtggcg gcgaaagcct gagcgtcgca
     9241 caggttctcc acgaagttgt tccggccttc gaagcgttgc gcaaggcggg caagacgcgc
     9301 ttcctgggca tcaccgcggt cggcgaaacg gcggccctgc atcaggttat cgatgccgaa
     9361 gtgatcgata gcgcccaggt cagctacaac atgctgaacg cctccgccgc aaccgcgctg
     9421 ccggcgaact atccggcgca ggactacggc cgcctgttcg accataccca aaaggccgga
     9481 gtcggcgtcg tcggcattcg ggttttggcc gcgggtgcgc tgtccggcac ctccgaacgc
     9541 cacccgaccg ctggcgcgcc gcccgaaccc atcggttcgg ctcacagtta tgaaatggac
     9601 ctggagcgcg cccgccggct gatgccagtg gtgacggaag gtttcgcctc cagtctggcc
     9661 gaggcggcaa cccggttcgc catcacgcac ccggccatgg gtacgattct tgtcgggatg
     9721 gcgaccgtcg agcagttcca ggccagtctc gacgcggtgc tgaaagggcc tttgccgcaa
     9781 gccgcactgg atcggttgag cgttttgacc gcagggtttg caggagagcc gcgttagcgc
     9841 gcgtcatgtg tcgcccgtta acttttcaca tctggcgact tacgcgaatt ggcgcgattc
     9901 gaaggacggt ggcgccctca tgcaggggtg agttcaaggg cgcctgaacc tgtcccctgc
     9961 catcacgatc atgcgcgggt caaccgatgc tctgcaggac agcagccgct aatttcggct
    10021 acctgtttga accgtccagc cgagttatcg cggcggcatc cggaacgctc cgtccagccg
    10081 gatcacctct ccgttcagga accggtttcg cacgatgtgc tccaccagtt cggcgaactc
    10141 cgacggccgt cccagacgcg gtggaaacgg cacccccgcc ccgaggctgg cttgtacctc
    10201 cggcggcagc ccatccacca tcggcgtctg aaacagcccc ggcgcgatcg tcaccacacg
    10261 caccccggcc ctggccaact cgcgcgcggc cggcaacgtc atcgcgacca cgccaccttt
    10321 ggacgcagcg taagccggct gcccgacctg gccctcgaac gccgcgatgg acgcggtgtt
    10381 gacgattatg ccgcgctcgc cttcctccaa cgcctcggcg gccgacattt ccgctgccgc
    10441 caggcgcagc atgttgaagc tgccgatcag gttgacgcgg atgacccgtt cgaacgcctc
    10501 cagcttcatc ggcccgtcgc gtccgacgat ccgccccgcg gtgccgatgc cggcgcagtt
    10561 caccaggatc cgcaccttcc catgcgccgc ccgagcggcc gcgaacgccg cttcgccgga
    10621 tgcggcatcg gccacatcgc atcgcacgcc gatcccgttg atcgaggcgg caacggactc
    10681 cgccgccgcc tggttgatat ccgcgaccgc caccttgcag cccaacgccg ccaggtgcgc
    10741 cgccgtcgcc gccccgaggc ccgaaccgcc gcctgtcacc agtgccgcca aatccttgag
    10801 ctgcattatg tcctccacca ccctgaccgg gcacaaacca cccggcgcaa accggcgcaa
    10861 ccctcgggcg ccaggccgcc ttgaccgccg aacgatccag cccgaaaacc gtcgcaagga
    10921 acacgccgcg atgaccccag ggagcaacgt gccatgacca ctgccccaac ctatgcggac
    10981 gcttacgaag actgggcgcg cgacgcctcg gcctattggg cgcgcgaagc ccagggcatt
    11041 acctggccga aaacctggga taaagtattc gaacccgggt tgggtgcgtt cggccagtgg
    11101 ttcgccggcg gaatgctcaa tacctgctac aactgtctgg atcgtcatgt cgaagccgga
    11161 cggggtgagc aggcagcttt gatccacgac agcccaatga ccgacagcgt caggacgttc
    11221 acttaccgcg acctgctcga gcgcacggcg cgcctggccg gagccctttc ggcgcttggc
    11281 gtcacaaaag gcgaccgggt tgtcatctac atgcccatgg ttcccgaagc cgccatcgca
    11341 atgctggcgg tcgctcggct tggggcggtg cactccgtcg tcttcggcgg cttcgcggcg
    11401 gcggaactat cgacacgtat cgcggacgcc aagcccaagg tcatcgtgtc cgcatcgtgc
    11461 ggcctggagc cgggcagggt ggtgcaatac aaacccctgc tggatgccgc catcgcactg
    11521 tcgccgcaca agcctgacgc ctgcctgatc ctgcatcgcc cgcaggccgg agcgcctatg
    11581 acgccaggcc gcggccatga ttttaatgcc gcggtggacg cagccgaacc gcatggctgc
    11641 gtcgctgtcc tggcaaccga tccgctgtac attctataca cttccggcac caccggccgc
    11701 cccaaaggtg tggtccgcga caacggcggc cacgccgtcg tgctgtggaa ctcgatgaag
    11761 atgatctacg ggttggacga aggcgacgtg ttctggaccg cttccgacgt cggctgggtc
    11821 gtcggccaca gctatatcgt ttacgctccg ctgctccgcg gctgcacctc ggtgatgtat
    11881 gagggcaagc cggtcggcac cccggatgcc ggcgccttct ggcgggtgat cgcccagcac
    11941 cgggtcaagg tgctgttcac cgccccgaca gccatgcgcg ccatcaagca gcaggacccc
    12001 gaaggccgat tggcccgatc ccatgacttg tcgagcctgg aggcgcttta cctggccggc
    12061 gaacggtgcg acccgccaac cgccgtctgg gcgcatgaac tgttgggcaa accggttgtc
    12121 gatcactggt ggcagaccga gacggggtgg ccgatcaccg ccggctttcg ccagttcggg
    12181 ctgttcccgt tcaaacccgg ttcgggggga cgtccgtgtc ccggctatga cctgcatgcg
    12241 ttggacgatg atggcaaggt tctgccggcg ggggagaccg gcaatctgtc gcttcgcctg
    12301 ccactgccgc cgggctgcgg tcccacgcta tggcagaacg atgagggcta ccgcgcgtcg
    12361 tacctggccg actttcctgg ctggtaccgc accggtgacg ccggcatggt cgataccgac
    12421 ggcgacgtct gggtcatggg ccgcaccgac gacatcatca acgtcgccgg ccatcgattg
    12481 tccacgggat cgatggagga agtcctcgcg tcgcacggcg atgttgccga atgcgccgtg
    12541 atcggcgcca aagacgagct gaagggccag accccgatcg gcttcgtggt cctgaaggcg
    12601 ggcgttgacc ggcccgaagc ggaagttgcc gccgaactcg tggcgctggt gcgggaacgg
    12661 atcgggccgg tggcgtcgtt cagggacgcg cgggttgtgg caagactgcc gaagacccgg
    12721 tccggaaaga tcctgcgggc gagtatccgc aagatcgccg atggcgagga agcggcggtg
    12781 ccgccgacga tcgaggatcc ggcggcactc gacgaactca ggctggtgct gcaacggtag
    12841 caaacgccca aggatgtcgc gcgggtgctt ggtcgtggtg cgacacggca ccggtttata
    12901 tggaattact atatcccggc cgctcaagcc acatcgcttc tttagtctcg tgcgtctaat
    12961 tcatcggcct gacaaccggc tttgggagca ttgacatgct tgacgtcgca ttcctcgcaa
    13021 ctggtgcggc cttcatgatc gtctgcgtac tgtacgcgca cgcttgcgat tatctttagg
    13081 ccacgccgat gattctggat tacgcgctgg gcggcatcgt gaccgtcttc ctgcttgcct
    13141 acctggtcgt cgcgctgatc aagccggaac gcttctgata atcgcgatct gggtaaagaa
    13201 gcgcgccgga ctaattcctg gcgtccgata aacgggccac tgcacctgac gccaggaatc
    13261 aggcgtcgag gccggaccac catgttccgt tccttttatg gaataataga atgacagtca
    13321 atggctggct ccaggttgcg ctgttctgcg tcctgctggt gctcctaacc aaaccgttgg
    13381 ggggctacat gacgcgagtc ttcgcagggg acaaaacgct gctgtccccg gtgctgagac
    13441 cgctggaaac atttctttat cgcctttccg gcatcgatga aaggctggaa cagcattggg
    13501 ttacctacgc cgtcgccatg ctggcgttca gtttcgccgg cttcgtcgtg ctttacgcat
    13561 tgcagcggct gcaggcgatc ctgccgctga atcctcagca cctggacgcg gtctcgccgg
    13621 acctcgcgtt caatacctcc gtcagttttg tcaccaatac caactggcag tcctacgtgc
    13681 cggaaaccac gctcagctac ctagtgcaaa tggcgggatt gacggtgcat aacttcgtca
    13741 gtgccgccac cggcatcgct ttggcgatcg cattggtccg gggctttacg cgccgctcct
    13801 ccgccaccgt tggaaacttc tgggtcgata tgacccgctg cacgctgtac gtactgttgc
    13861 cggcaagtat tgtattcggg ctcgtcatgg tttcgcaggg cgtgccgcaa aacctgtccg
    13921 cctataccga cgtcacgacg cttgaaggtg ccaagcaggt catagcccag ggtccggtcg
    13981 cttcgcagga agtcatcaag atgctgggaa ccaacggcgg cggcttcttc aatgccaact
    14041 ccgctcatcc gttcgagaac ccgacggcgc tgaccaacct gctccagatg ctgctgatct
    14101 tctccatcgg cgcggcattg accaacgtgt tcggccgcat ggccggcaac cagcgccagg
    14161 gctgggcgat cttcgccgtg atgggggtaa tgtttctcgc cggagtcacg gtgctctacg
    14221 cgtctgaagc cgccggaaag gccctgcttg cgcatctgcc gctcgaccag cttgccggca
    14281 acatggaag
//
LOCUS       DASWQN010000540         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_975711, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000540 DASWQN010000000
VERSION     DASWQN010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975711"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VGC82_09040"
     CDS             complement(<1..229)
                     /locus_tag="VGC82_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0423451.1"
                     /translation="MPVALLSGFLGSGKTTLINALLRDPRMAGTAVAVNEFGEIPLDR
                     DLIDHGADKTVVMANGCLCCNLAGDMEDAVMR"
     gene            complement(271..1710)
                     /locus_tag="VGC82_09045"
     CDS             complement(271..1710)
                     /locus_tag="VGC82_09045"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0423452.1"
                     /translation="MPARTGAEFLHGLRDGRELWLGGQRVSDPMEHPALRGAARAIAD
                     VYDLHYQFPDDCLMPDPETGEPIAVSHIIPRSKDDLLRRHKALRRVAEFSVGLMGRTP
                     DYMNVTYAGFAGRQDEWAINGNEEGAANLVAFQKQLARQDLSLTHTLIHAMSDKSKGH
                     VPAAFDETQLHTVAKTEHGILVKGARVLATLAPFSDELAVYPSQPMPDASRRHALSFC
                     IRMDTPGLKFICRDSVSLPDNRFDHPLSGRFDEQDAFVIFDNVEVPRNRVFIDGNLPV
                     YNSVMSASWRANAMHQTMIRAWTKLEFAWGIANRMADAIGASDPSTLQMLGEIWSYAE
                     LARSAVMAGEQGAYEYGNGAWFLDGRPMDALRTSLPYWFPRVNEIIRLIGSHNVLATP
                     ASGAFDDPQLRPLIDKYLRGAGDTTAFDRARIFRLGWDFAGSALASRNEQYERFYLTS
                     GARNRQNVQLRADRSEADRLVDRFLREKT"
BASE COUNT          322 a          592 c          664 g          394 t
ORIGIN      
        1 ggcgcatgac agcgtcttcc atgtcgcccg ccaggttgca gcacaggcag ccgttggcca
       61 tcacgacggt cttgtcggca ccgtggtcga tcaggtcgcg gtcgagcggg atctcaccga
      121 attcgttgac cgccacggcg gtgccggcca ttcgcggatc gcgcaacagg gcattgatca
      181 gcgtggtttt gccgctgccc aggaagccgc tgagcagcgc caccggcatg cggtcgcggt
      241 cgacggtttg gctcaggaag gaaaaccggt tcacgttttt tcccgcagaa accgatctac
      301 gaggcggtct gcctcggatc ggtcggctcg cagttgcacg ttctgccggt tgcgtgcgcc
      361 ggaggtcagg tagaaacgtt catattgttc gttgcgactg gccagggcgc tgccggcgaa
      421 atcccagcca aggcggaaaa tgcgggcgcg gtcgaacgcg gtggtgtccc ctgctccgcg
      481 caggtatttg tcgatcaacg gacgcagttg gggatcgtcg aacgcgcccg aagcgggcgt
      541 tgccagcacg ttgtgggagc cgatcaggcg gatgatctcg ttcacccgcg ggaaccagta
      601 cggcagggat gtgcgcagcg cgtccattgg ccttccgtct aggaaccagg cgccgttgcc
      661 gtattcgtac gcgccctgct cgccggccat cacggccgaa cgggccagtt cggcgtagct
      721 ccagatctcg cccagcattt gcagggtgga aggatcggac gcaccgatgg cgtcggccat
      781 gcggttggcg atgccccagg cgaactccag cttggtccac gcccggatca tcgtctggtg
      841 catcgcgttg gcgcgccagc ttgccgacat gacactgtta tagacgggca ggttgccgtc
      901 gatgaaaacg cggtttcgtg gcacctccac gttgtcgaag atgacgaagg catcctgctc
      961 gtcgaaccgc ccggacaaag ggtggtcgaa gcggttgtcg ggcagggaca cgctgtcgcg
     1021 gcagatgaat ttcaggccgg gggtgtccat tcggatgcag aaggacagcg catggcgccg
     1081 gctggcgtcg ggcatcggct ggctgggata aactgcgagc tcgtccgaga acggtgccag
     1141 cgtcgccagc acccgcgcgc ctttcaccag aatcccatgc tcggtttttg ccacggtgtg
     1201 caactgggtt tcgtcaaacg cggcagggac atggcctttg gacttgtcgg acatggcgtg
     1261 gatcagcgta tgggtcaggc tgaggtcctg gcgcgcgagt tgcttctgga acgccaccag
     1321 gttagctgcg ccttcttcat tgccgttgat cgcccactcg tcctggcggc cggcgaatcc
     1381 ggcataggtg acgttcatgt agtcgggcgt gcggcccatc aggccgaccg agaactcggc
     1441 gacccggcgc aaagccttgt ggcggcgcaa caggtcgtcc ttcgagcgtg gaatgatatg
     1501 gctgacggcg atcggctcgc cggtttccgg atcgggcatc aggcagtcat cggggaactg
     1561 gtagtgcagg tcgtacacgt cggcgatggc ccgggccgcg ccacggagtg ccgggtgctc
     1621 catcgggtcg ctgacgcgct gaccgcccag ccagagttcg cggccgtcgc gcaggccgtg
     1681 caggaattcc gcccccgtgc gtgctggcat cgccgtgttc ctcccgttcg tctcatgatt
     1741 aggactttcc tggggtgagt gggaagccat gtgcgttgga ccggtgtttc tggcgcggcc
     1801 tacgtgccgt tggactgtgt taccgccata aatctacgca aaactttcgg cttgccgata
     1861 tgatgtggct ggacagccga ggcttgagga ctcaagactc tgcatgacgc tttcgctata
     1921 ggtcctgtcc tgacgcttcg cccgcttcgt tcccatcaac agcgcctcgt cg
//
LOCUS       DASWQN010000541         5768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1240278, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000541 DASWQN010000000
VERSION     DASWQN010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240278"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VGC82_09050"
     CDS             complement(<1..328)
                     /locus_tag="VGC82_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423453.1"
                     /translation="MPDAPPLRPASADEIAESLSFALRYDGRKRVHDAADVMARITAD
                     RLVRHLQQAGFVLMKSAPAPAPTTAGCLRGNRTDEQAVRRTLLRPWRLPWLKRRPMVY
                     LSIENFV"
     gene            complement(458..769)
                     /locus_tag="VGC82_09055"
     CDS             complement(458..769)
                     /locus_tag="VGC82_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HEY0423454.1"
                     /translation="MSAPEFDTATRTELEAAAFRRLVQHLRERTDVQNIDMMNLAGFC
                     RNCLSRWYREEAATKGITIADPDAREIVYGMPYKEWQSKHQSEASPEQKAAFAKASPG
                     H"
     gene            862..2193
                     /locus_tag="VGC82_09060"
     CDS             862..2193
                     /locus_tag="VGC82_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEY0423455.1"
                     /translation="MHQDHAWADDENWPPDDRRADTRARLALRTLFDTAVAAADPRSV
                     LARHLPPKPASGRCVVVGGGKSAAVMAAALEDAWPDVALEGTVVTRYGHAVPTRLIEV
                     IEASHPVPDANSELGAKRLLERVRHLTKDDLVIALMSGGASALLAAPVPGLTLSDKQA
                     INRALLACGANITEMNAVRKHLSAIKGGRLAAAAAPARVVTLAISDVPGDDPAVIGSG
                     PTVADATTFAQARSLIARYGITPPQAVAARLARDDDETPKPGSLANVEFRMIATPMMA
                     LDAMAAKARTLGLTPLILGDALEGEASQMGTVMAGIARSVRAHGHPVRRPAVLLSGGE
                     TTVTMRGAGTAVGKGGRNTTFLLGLAIALDGAEGIWATAGDTDGIDGMDDVAGALLAP
                     DTLARARGKGLEARAMLASHDSHGFFGAIGDLIVTGPTLTNVNDIRAVLIT"
     gene            2272..3699
                     /gene="pyk"
                     /locus_tag="VGC82_09065"
     CDS             2272..3699
                     /gene="pyk"
                     /locus_tag="VGC82_09065"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY0423456.1"
                     /translation="MRPKMRRRRRTKIIATLGPSSSTLEVMTRLFQAGADVFRLNFSH
                     GTHEDHAARFATIRELEKQFDRPIGILADVQGPKLRVGRFGGGRVHLQTGQTFRLDLN
                     ATPGNAQRVNLPHPEIIEAAAIGSSLLLDDGKLRLRVVHKRADALETEVVVGGPLSDR
                     KGVNVPDVVLPIPALTVKDREDLEFAVSHGANYIGLSFVQRPEDVIEAQRLIAGRAWV
                     MVKLEKPQALDNLEEILALTDAVMVARGDLGVELPPEEVPLAQKRIVRLARALGKPVV
                     VATQMLESMISAPAPTRAEASDVATAVFDGADAVMLSGETAAGQYPYEAVNMMDRIVA
                     RVEQDAGWRALIDASRVPSGKNAADAIAAASSQVGDTIGAKAIVAFTASGGTALRVAR
                     ERPVAPVIGLTPNPAIAQLMAVVWGVHAVVVPDVHSMTEAVNRASRVALTEGFATHGD
                     EIVVTAGVPFGHSGTTNALRVATVK"
     gene            3824..4153
                     /locus_tag="VGC82_09070"
     CDS             3824..4153
                     /locus_tag="VGC82_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003625676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0423457.1"
                     /translation="MKTKHDWSRLDAMTSEQRHAAALADPDARPQTDANMDRMTPTPR
                     LKIIRRALGLTQEEFAVRYRIPLGTLRDWEQGAKQPDQAARAYLRAIAGDPAAVQRAL
                     HTTPQLS"
     gene            complement(4240..4590)
                     /locus_tag="VGC82_09075"
     CDS             complement(4240..4590)
                     /locus_tag="VGC82_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423458.1"
                     /translation="MRRVWVLFLAGLSAGCVAGLATRQAELSQWIGRPETELAAAMGA
                     PNRTYDSGGMKFLTYEERRVEIVPETPYYFGPGPFGYGSPFPPTATTLQCDTTFTVAG
                     GVVRAFSLRGNACG"
     gene            complement(4739..5746)
                     /gene="epmA"
                     /locus_tag="VGC82_09080"
     CDS             complement(4739..5746)
                     /gene="epmA"
                     /locus_tag="VGC82_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HEY0423459.1"
                     /translation="MALPIWHPESLLARLPFLHRRAELTRSVRGYFLKRGYMEVETPY
                     AVPAPGEEVHLRTFATTRERTDGASEPLWLHTSPEFAMKRLLVAQAGPIFQFARVWRN
                     GEGGALHAAEFTMLEWYRPETGMDALINEVVELLRTVLPAVVRCRGITSDLSRFERLT
                     VAEAFTRHVGVDLLAIGEDAAGLAAAARVRLRKGESWEDLFFRLLLERIEPHLGRQHP
                     TFLTHWPAAQAALARRDPADPRVAERFELFVCGVELANAFVELTDPVEQRARFLQERE
                     RRHAMRGPDWALDEDFLAALEFGMPPSAGIALGFDRLAMIASGAERIGQVLWLPPADL
                     L"
BASE COUNT         1023 a         1941 c         1860 g          944 t
ORIGIN      
        1 ctacgaaatt ttcaatagac aaataaacca ttgggcggcg tttgagccaa ggcaagcgcc
       61 atggacgtaa cagtgtacgc ctcactgcct gttcatccgt tcggtttccc cgaaggcatc
      121 cggcagtcgt gggggccggc gccggcgcgg atttcatcag cacgaagccg gcctgctgaa
      181 ggtgccgtac cagccgatct gcggtgatcc tggccatcac gtccgctgcg tcgtgaaccc
      241 gctttcggcc gtcgtagcga agcgcgaagg acaaggattc agcgatctca tcagcggacg
      301 ccggccggag agggggtgca tcgggcattc cgcgatgcta tcaggtcgga taggaacata
      361 tcaagaacct tgccggcggc cggtctcgcc gcgtacgcga cctgtctcgc cgcatacatg
      421 gctcgatcgc cttgcggccc gaagagtcac gccaacatca gtggcccggg ctcgccttcg
      481 caaaggccgc cttctgctcc ggtgaggcct cggactgatg tttggactgc cactccttgt
      541 aaggcatccc gtacacgatc tcccgtgcat ccggatcggc gatcgtgatc cccttggtcg
      601 cggcttcttc gcgataccag cggctcaggc agttccggca gaatccagcc aggttcatca
      661 tgtcgatgtt ctgcacgtcg gtgcgttcgc gcaaatgttg caccaggcgg cggaacgcgg
      721 cggcctccag ttccgtccgg gtcgcggtgt cgaattcagg tgcggacatg gttgtctccc
      781 gttgctgcgg cgatgacctg aagatggcgc acctcgccgg gcttcgccat actcctcgct
      841 gcaaaaagag aggaaccgtg gatgcaccag gatcatgcct gggccgatga cgaaaattgg
      901 cctcctgacg atcggcgggc cgacaccagg gctcggttgg cgttgcgcac cctgttcgac
      961 accgccgtcg cggcggccga cccgagatcg gtgcttgcgc gccatctgcc gccgaagcca
     1021 gcttccggcc gctgcgtcgt cgtcggaggc ggcaaatccg ccgctgtcat ggctgccgcc
     1081 ttggaggacg cctggcccga tgtcgcgctg gaaggcaccg tcgtcacccg ttacggtcac
     1141 gccgtgccga cccgtctgat cgaggtgatc gaagcctcgc accccgttcc cgacgccaac
     1201 agcgagttgg gcgccaagag actgctcgag cgcgtacggc acctgaccaa ggacgacctc
     1261 gtcatcgccc tgatgtcggg cggcgcctcc gccttgctgg ctgcgccggt gcccggcctg
     1321 accctgtccg acaaacaggc gatcaacaga gcgctgctgg cctgcggcgc caatatcacg
     1381 gagatgaacg ccgtcaggaa acatctgtcg gcgatcaagg gcggacgtct tgccgccgcc
     1441 gccgcccccg cccgggtcgt caccctggcg atcagcgacg tgccgggcga cgatcccgcc
     1501 gtcatcggtt ccggtcccac tgtcgccgac gcaacgacct tcgcacaggc ccgaagcctg
     1561 atcgcccgct acggaataac gccgccgcag gcggttgccg cccgcctggc ccgggatgat
     1621 gacgaaacgc cgaaacccgg atccttggcg aacgtggagt tccgtatgat cgccactcca
     1681 atgatggccc tcgacgcgat ggcggccaaa gcccgcacgc tcggccttac gcccctgatc
     1741 ctcggtgatg ccctggaggg tgaggccagc cagatgggca ccgtcatggc cgggatcgcg
     1801 cgctccgttc gcgcccacgg ccatccagtg cgacgacccg ccgtgctgct gtcgggcggc
     1861 gagaccaccg tcaccatgcg gggagcggga acggccgtcg gcaagggtgg acgcaacacg
     1921 acgttcctgc tgggcttggc catcgcactc gacggcgccg aaggcatctg ggcgaccgcc
     1981 ggagacaccg acggcatcga cgggatggac gacgttgccg gcgcgcttct ggcgccggac
     2041 acgctggccc gtgcccgcgg gaagggcctc gaagcccgcg ccatgcttgc cagccacgac
     2101 agccatgggt tctttggcgc gatcggggac ttgatcgtca ccggcccgac gctcaccaat
     2161 gtgaacgata tcagggccgt cctgatcacc tagagcaggt cccgttccgg cgccggcagc
     2221 aacggccggt gggtcaattt ctgaaacaac gcgagggaat cgcttatggc catgcgcccc
     2281 aaaatgcgcc gccgccgccg cacgaagatc atcgccaccc tcggcccctc cagttccacg
     2341 ttggaggtca tgacgcggct gttccaggcc ggcgccgatg tgttccggct gaacttctcg
     2401 catggcacgc atgaggacca tgccgcccgc tttgccacca ttcgcgagct ggagaagcag
     2461 ttcgaccgcc cgatcggcat cctggccgac gtgcaaggac cgaaactgcg ggtcggccgc
     2521 tttggcggcg ggcgggttca tctgcaaacc ggccaaactt tccgcctgga cctgaacgcc
     2581 acgcccggca atgcccagcg ggtcaacctg ccgcatccgg agatcatcga agctgccgct
     2641 atcggctcct cgctgctgct tgacgacggc aagctgcgcc tgcgtgtcgt ccataaacgt
     2701 gccgacgcgc tggaaaccga ggtcgtggtc ggcggcccgc tatccgaccg caagggtgtc
     2761 aacgtccccg acgtggtgct gccgatcccc gccctgaccg tgaaggaccg agaggatctg
     2821 gaattcgccg tctcgcacgg cgccaactac atcggtcttt cgttcgtcca gcgccccgag
     2881 gacgtgatcg aggcgcagcg cctgattgcc ggccgcgcct gggtgatggt gaagctggaa
     2941 aagccgcagg cgctggataa ccttgaggaa atcctggctc tgacggatgc ggtgatggtg
     3001 gcccgaggtg atctcggggt cgaactgccg cccgaggaag tgccattggc gcaaaaacgc
     3061 atcgtccggc tggcgcgcgc gcttggcaaa ccggtcgtgg tcgcgaccca gatgctggaa
     3121 agcatgatca gcgccccggc tccgacccgc gccgaggcct ccgacgtggc aaccgcggtg
     3181 tttgacggcg ccgatgcggt aatgctgtcg ggcgagaccg cggccggcca gtatccgtac
     3241 gaggcagtca acatgatgga ccgcatcgtc gcccgggtgg agcaggacgc cggctggcgt
     3301 gcgctgatcg atgcctcgcg cgtcccatcg gggaaaaacg cggccgatgc gatcgccgcc
     3361 gcatcgtctc aggtcggtga cacgatcggc gccaaggcca ttgtcgcatt cacggcctcc
     3421 ggcggcaccg cgctacgcgt cgcccgcgaa cggccggtgg caccggtgat cgggctgacg
     3481 ccgaaccccg cgatcgcaca actgatggcg gtggtttggg gtgtgcatgc cgtggtggtg
     3541 ccggacgtgc actcgatgac cgaggcggtg aaccgcgcca gccgcgtagc cctgaccgag
     3601 ggtttcgcca cccacggcga cgagatcgtg gttaccgctg gcgtcccgtt cgggcattcg
     3661 ggcaccacaa atgccctgcg ggtggcgacg gtgaagtagc gcgacggcgg ttaggccata
     3721 gcattgttgt cgggcaggtt cccgcctcca gaccttccag cgattgcttt acacccgcgg
     3781 gggagccgcg aagcggaacc tcgtgaacga cggaggtatc acaatgaaaa ccaagcatga
     3841 ctggagccgt ctcgatgcga tgaccagcga gcaacgccat gccgccgcgc tcgccgatcc
     3901 ggacgcacgg ccgcagaccg acgccaatat ggatcgcatg acgcctacgc cacgcctgaa
     3961 gatcattcgt cgcgcactcg gccttacgca ggaggaattc gcggtgcggt atcggatacc
     4021 gctcggcacg cttcgggatt gggagcaggg ggccaagcag cccgatcagg cggcacgtgc
     4081 ctatctccgg gcgattgccg gtgatccggc ggcggtgcag cgggcactgc atacgactcc
     4141 ccagctaagc tgagagagcc catgccactc gccagatcta gcgggctatt tcagaaaagc
     4201 gcaatttggc taagcccgac aggctgctaa ggccggcgct cagccacagg cgttcccccg
     4261 caggctgaac gcccgtacga cgccgccggc aactgtgaac gtcgtgtcgc actggagcgt
     4321 ggtcgccgtt ggcgggaagg ggctgccata gccgaatggc ccaggaccga agtagtacgg
     4381 cgtctccggc acgatctcga cgcgacgctc ctcgtaggtg aggaacttca tgccgccgct
     4441 gtcataggtt cggttcggtg cgcccatggc cgcggccagt tcagtttccg gccggccgat
     4501 ccactggctc aattcggcct ggcgggtggc cagcccggcg acacagccag ccgacagccc
     4561 ggccaggaaa agaacccaaa cgcggcgcat cggcttgctc cttgctggcc acctcatacc
     4621 gacggcctta aatattgacc cgctggcagg tcagcttccg tcggtatcct gttgatttgg
     4681 cgcgcagccg ccacaccaac cctgcgcgca caccagtcaa cccttcaggc tgctggtatc
     4741 ataaaagatc cgccggtggc agccatagca cctggccgat ccgctcggca ccggaggcaa
     4801 tcatcgccaa ccggtcaaat ccgagtgcaa ttccggcgct gggcggcatg ccaaattcca
     4861 gggccgcgag aaagtcttcg tccaaggccc aatccggacc tcgcatcgca tgacgccgct
     4921 cgcgctcctg caaaaaccga gcgcgctgtt ccaccggatc ggtgagctcg acaaatgcat
     4981 tggcgagctc gacgccgcag acgaaaagct cgaaccgctc cgcgacgcgc ggatcggcgg
     5041 gatcgcgtcg cgccagcgcc gcctgggccg ccggccagtg ggtcaggaag gtcggatgct
     5101 gccgcccgag atgcggttcg atccgttcca gcagcaggcg gaagaacagg tcctcccagc
     5161 tctcgccctt gcgcaacctg acgcgcgccg ccgcggccag tccggctgca tcttcgccga
     5221 tcgccagcag atcgacgccg acatgccggg tgaatgcctc ggcgacggtc agacgctcga
     5281 aacgcgacag gtcggaggtg atgccgcggc agcgcactac tgccggcaaa accgtacgca
     5341 acagttccac gacctcgttg atcagtgcgt ccattccggt ttcagggcgg taccattcca
     5401 gcatggtgaa ctcggcggcg tgcagcgcgc cgccctcgcc gttgcgccag acccgagcga
     5461 actggaagat cgggccggcc tgcgccacca gcagccgctt catggcgaac tccgggctgg
     5521 tgtgcagcca gagcggttcg gatgcaccat cagtgcgttc acgcgtcgtg gcgaaggtcc
     5581 gcagatgcac ttcctcgccg ggggcgggaa ccgcatacgg ggtttcgacc tccatgtaac
     5641 cgcgcttgag gaaatacccg cgcaccgacc gggtcaattc cgcccgccgg tgcaggaacg
     5701 gcaaccgggc gaggaggctt tcaggatgcc agataggaag tgccatgccg gaatgtttac
     5761 cgcgctga
//
LOCUS       DASWQN010000542         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1636148, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000542 DASWQN010000000
VERSION     DASWQN010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636148"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..690)
                     /gene="tsaB"
                     /locus_tag="VGC82_09085"
     CDS             complement(31..690)
                     /gene="tsaB"
                     /locus_tag="VGC82_09085"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY0423460.1"
                     /translation="MRILALDSAVGRCSATIVTDGEVVVGRQQDIERGHASILPVMAE
                     QCLRRSGIKASDLDLIAVTVGPGSFTGIRGGVALAQGIAVAANRPVVGVTVGEALADL
                     LPQLGGRTLWCAIPSRRGRIFLEVGAEIFSLAVTELPNPGGSVAVAGGAATDIASRLA
                     ARGVNVMLTDARLPTGRSIAVVAQRRFAGTIRPLPAEPIYIDAPEAKLPGGRTTPFGT
                     G"
     gene            complement(787..1374)
                     /locus_tag="VGC82_09090"
     CDS             complement(787..1374)
                     /locus_tag="VGC82_09090"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEY0423461.1"
                     /translation="MTIDAAGLELLFKEARSHNAWTSEPVSDETLHELYDILKFGPTS
                     ANSSPARFVFIRTKEGKEKLAPALSAGNLNKTMSAPVTAIVAYDPKFYEKLPTLFPHN
                     PDAISWFTSNDSLAATTAFRNGTLQGAYLLMAARSLGLDVGAMSGFDNAKVDAAFFAF
                     NGWRSNFLVNIGHGDPAGLFGRSPRLPFDEACLLA"
     gene            complement(1442..2002)
                     /locus_tag="VGC82_09095"
     CDS             complement(1442..2002)
                     /locus_tag="VGC82_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEY0423462.1"
                     /translation="MFIETESTPNPATLKFLPGRDVMGFSTADFASPVSAIRSPLATD
                     LFALPGVARVFLGGDFITVTKNDEISWQALKPQVLGAIMDHFVSGRPMLEGDAADFSE
                     EDVDEADREVVDQIKELLDTRVRPAVAGDGGDIVFRGYREGVVRLHMQGACSGCPSSS
                     ATLKHGIENMLKHYVPEVTAVEQVEF"
     gene            complement(2149..2964)
                     /locus_tag="VGC82_09100"
     CDS             complement(2149..2964)
                     /locus_tag="VGC82_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY0423463.1"
                     /translation="MRSPRVVKPLIGISCCTKQFGIFGMPNHAASDTYVRATDEVIGA
                     VPVLLPANGPAADIETLLERLDGIVLTGSRSNVQPTLYAGPPHAEGTPEDPARDGITL
                     PLIRAAVARGVPLLAICRGFQELNVALGGSLHQRLQDLPDRMDHSTPMQPSSRVRQSK
                     AHAIRVTQGGWLHRLAGTMEIAVNSLHNQGIDQLAPGLIAEGIAPDGTIEAVRVVASQ
                     AGPALGYAVGVQWHPEYDWRTDKLSRAIFEQFGAAVRAYADAARLGGVSIAAD"
BASE COUNT          566 a          927 c          972 g          513 t
ORIGIN      
        1 ggtaaccgtc gctcggtaca tcatgcgatt tcaaccagta ccgaagggcg ttgtcctgcc
       61 gcctggaagc ttggcctcgg gcgcatcgat gtagatcggt tccgccggca atggacggat
      121 cgtgccggcg aatcgccgct gcgccaccac ggcgatcgac cggccagtgg gaagacgagc
      181 atcggtcagc atgacgttga cgccccgtgc ggccagccgg ctggcgatat cggtggcggc
      241 gcccccggcg accgcgacgg atccgccggg gttcggcagt tcggtcacgg cgagtgagaa
      301 aatttccgcg ccgacctcca ggaagatacg cccgcgacgg ctggggatgg cgcaccacag
      361 cgttcgcccg ccaagttgag gcagcagatc ggccagcgcc tcgcccacgg tcacgccgac
      421 cactgggcga ttggccgcga cggcgatgcc ttgggccagg gccacgccgc cgcgtatgcc
      481 ggtgaaactg cctggcccga cggtcaccgc gatcagatcg aggtcggacg ccttgatgcc
      541 cgaacgccgc aggcattgct ccgccatcac cggcaggatc gacgcgtggc cgcgctcaat
      601 gtcctgctgg cggccaacaa cgacttcgcc atccgtgaca atggtcgccg agcatcgtcc
      661 aacggccgag tcgagggcga gtatccgcat gatcggcaaa atctagcctg ccgacggggc
      721 gacccgccag catgatcaat tctgtcaggc gagaagacag gccccctcca ggcgagacag
      781 gccccgtcag gcgagaagac aggcctcgtc gaacggcagc cgcggcgagc ggccgaacaa
      841 tccggcagga tcaccgtggc cgatgttgac caggaagttc gaccgccagc cattaaaggc
      901 aaagaaggcg gcatcgactt tggcgttgtc gaacccggac atcgcgccaa cgtccagacc
      961 cagtgatcgc gccgccatca gcagataggc gccttgcagc gtgccattgc gaaaagccgt
     1021 ggtcgctgcc agcgagtcgt tgctggtgaa ccaggagatc gcgtccgggt tgtgcgggaa
     1081 caacgtcggc agtttctcgt aaaatttcgg gtcgtaggcg acgatcgcgg ttaccggagc
     1141 gctcatggtc ttgttcaggt taccggccga caaggcgggc gcgagcttct ccttgccttc
     1201 tttcgtgcgg atgaacacga agcgggccgg cgaactgttg gccgatgtcg gcccgaactt
     1261 caggatgtca tacagttcgt gcagcgtttc atcggatacc ggctccgaag tccacgcgtt
     1321 atggctgcgg gcttccttga acaacagttc caaacccgcg gcgtcgatcg tcatatacag
     1381 ctcctgcgtg atgttgcacc gcagcgtgga agttgcggtg caggccgtca atcggcgctg
     1441 gtcagaattc gacctgctcg acggctgtga cttccggcac gtaatgcttc agcatgttct
     1501 cgatgccatg cttcaatgtg gcactggatg acgggcaccc ggagcaggca ccctgcatgt
     1561 gcagccggac gacgccctcg cggtagccgc gaaagacgat atcaccacca tcgccggcga
     1621 ccgccgggcg aacgcgggtg tcgagaagtt ccttgatctg gtccacgacc tcacggtcgg
     1681 cttcgtccac atcttcctcg ctgaagtccg cggcatcgcc ttcgagcatc ggccggccgg
     1741 agacgaaatg gtccatgatg gcgcccagca cctgcggctt cagggcctgc caggatattt
     1801 cgtcgttctt ggtcacggtg atgaaatcgc cacccaggaa tacgcgggcg acgccaggga
     1861 gtgcgaatag gtcggtcgcg agcgggctgc gaatcgcgct gacgggcgag gcaaaatcgg
     1921 cggtggaaaa ccccatgacg tcgcgtcccg gcaggaactt gagtgtggcg gggtttggcg
     1981 tgctttcagt ttcgatgaac atgacgtggt tcccgtttta actctgggta tgaataatcc
     2041 ggaccgtcct ggtccggtct ctggacatac atcgggcgaa atcgatggac tgacaagggg
     2101 gaggtgaacg aagccgaccg ttggccacgg atggtcgggc aacggcgcct aatccgccgc
     2161 gatggaaacc ccgcccaggc gtgcggcatc ggcataggcg cgcaccgcgg ctccgaactg
     2221 ttcgaagatc gcgcgggaca gcttatcggt gcgccagtcg tattccggat gccattgcac
     2281 gcccacggca tatccgagcg ccggcccagc ctgggaagca acgacgcgaa ccgcctcgat
     2341 cgtgccatct ggcgcgatgc cttccgcgat caggcccggc gcaagctggt caattccttg
     2401 attatgcaga gaattcacgg caatttccat ggtcccagcc agacggtgaa gccagccgcc
     2461 ctgggtgacg cggattgcgt gcgccttcga ttggcgcacc cgcgatgacg gctgcatcgg
     2521 cgtggaatgg tccatccgat ccggcagatc ctgcagtcgt tgatgcagcg agccgccaag
     2581 cgccacgttg agttcctgaa agccgcggca gatagccaga agcggaacgc cgcgagccac
     2641 cgcagcacgg atcagcggca gggtgatgcc gtcccgggcg ggatcctccg gggtgccttc
     2701 cgcatgcggc ggcccagcgt aaagcgtggg ctgcacattg gaccggctgc cggtcaggac
     2761 gatgccgtcg aggcgctcca gcagcgtttc gatatcggcg gcgggtccat tggcggggag
     2821 caggacaggg accgcaccaa tgacctcatc ggtcgcccgc acatacgtat ccgacgccgc
     2881 gtggttcggc attccgaaga tgccgaactg cttggtgcag caactgatgc cgatcaacgg
     2941 cttgacgacg cggggagacc tcatgtcgag taagatta
//
LOCUS       DASWQN010000543         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1503881, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000543 DASWQN010000000
VERSION     DASWQN010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503881"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VGC82_09105"
     CDS             complement(<1..623)
                     /locus_tag="VGC82_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0423464.1"
                     /translation="MSYVDASALETQDRLVNDGLRSSLGPAVDLLERVEADAGDDGDD
                     GDDGDDIDLRQLRHHTKNTLQRILGLIATAPGLCETPAGEKIAQELEHRIALSANISN
                     ALFGLTGVPGSMAERLRQLSGSVVDMMRDADQVIRVGVLVRGVCPPALREAVLRSAHE
                     LLGNAVKHGMKGRPAGRIAVRLVSDCDMTVLSVVDNGWGFNGSPRSG"
     gene            858..1556
                     /locus_tag="VGC82_09110"
     CDS             858..1556
                     /locus_tag="VGC82_09110"
                     /inference="COORDINATES: protein motif:HMM:NF039473.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HEY0423465.1"
                     /translation="MPETVHLRPLPPPPLLKAARRLLRPLVRLMMHSGLTFPILADML
                     RALFVDVAVNDILADPKARTDSRISLLTGVHRKEIRRLRELPADRAAGIPDVITVGSQ
                     IIARWAGTPSFTDEAGHPRPLPRIHHDAADAEPSFDALVESITSDVRSRAVLDDLLGH
                     GIVSIDSNDRVQLNTAAFIPRPGGEEQLFYFARNLHDHVAAAVANISASGAPRFLDRS
                     VHLRPADVGTGPGA"
BASE COUNT          317 a          577 c          480 g          333 t
ORIGIN      
        1 tcacccgacc gcgggcttcc gttgaagccc cagccgttgt ccacaaccga cagcaccgtc
       61 atgtcgcaat ccgacaccag ccgaaccgcg atccgcccgg ccgggcgccc cttcatcccg
      121 tgcttcactg cgttgcccag caattcgtgc gcactgcgca gcacggcttc gcgcagggcc
      181 ggggggcaca ccccgcgcac caacacgcca acccggatca cctgatccgc atcgcgcatc
      241 atatcgacca ccgacccgga cagttgccgc aagcgttcgg ccattgagcc gggaacaccg
      301 gtcagcccga acagcgcgtt ggaaatgttc gccgacagcg cgatccggtg ttccaactcc
      361 tgggcgatct tttcgccggc cggcgtttcg cacagcccgg gcgcggtggc aatcagcccc
      421 agaatccgct gcagtgtatt tttggtgtgg tgacgcaact ggcgaaggtc gatgtcgtcg
      481 ccatcatcac catcatcacc atcatcgccc gcatcggcct caacccgctc cagcaggtca
      541 accgccgggc ccaaagatga cctcaaccca tcgttgacca gtcggtcttg cgtctcgagg
      601 gcggaggcgt cgacgtacga catgtcagga gcctcattgc gttgtcacat acagtagtcg
      661 gtggtcccgc actgtagcct tgttcgtcac acatcgtaac cttgaacgat taaaacaacg
      721 ttaagagcga ccgcagatgt caaacgaacc gtatgtggga aaatttccca cgtcaatcga
      781 attttgcatc ttgtgggtgt ttttcccaca tccagatatg ctatatcctt tctgagtaag
      841 gactattcca tcaaccgatg cctgaaaccg tccacctccg accgttgcca ccgccgccgc
      901 tgctgaaagc ggctcgccgg ctgctgcggc cattggtccg gctgatgatg cacagcgggc
      961 ttacgttccc gatcctggcc gatatgctcc gcgccctgtt tgttgatgtt gctgtcaatg
     1021 atatccttgc cgatccgaag gcgcggacgg acagccggat tagcctgttg accggggtcc
     1081 atcgcaagga aattcgacgc ctgcgcgaac tgccggccga tcgcgccgcc ggcatccccg
     1141 acgtcatcac cgtcggcagc cagatcatcg cccgctgggc cggcacgccg tcctttaccg
     1201 acgaagccgg ccatccgcgt ccgctgccgc gcatccatca cgatgcggcg gacgccgaac
     1261 cgtcgttcga tgctctggtc gaatcgatca cctccgatgt ccgatcgcgc gcggtgctgg
     1321 acgatctgtt gggtcatggc atcgtttcga tcgactccaa tgaccgtgtt cagctaaaca
     1381 cggcggcgtt tatcccccgg cccgggggtg aggagcagct gttctatttc gctcgcaatt
     1441 tgcacgacca tgtcgcggcg gccgtggcca acatcagcgc atctggtgcg ccacgctttc
     1501 tcgaccgaag cgtccattta cgaccggctg acgtcggcac aggcccagga gcttgaaaat
     1561 tacgcgcgcg cggcggccat gaaggtgctg ctcgatgtca accggcgggc gctcgcgatg
     1621 accgaatcgg cggcggatac cgccgctgcg gcgtcgaggc ggatcaatct gggcatcttc
     1681 gtgttcgacg acgacgatac gccgacc
//
LOCUS       DASWQN010000544         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1240468, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000544 DASWQN010000000
VERSION     DASWQN010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240468"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VGC82_09115"
     CDS             complement(<1..979)
                     /locus_tag="VGC82_09115"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423466.1"
                     /translation="MSPYCDQFGGAVLNTTIDRYAYAFIAPSDNGRVQFVAPDLAIEE
                     DFPLDLDALSQGRLGLHAGVARRMLTEFGDAKIRPMRITSYVDAPPGSGLGSSSALVV
                     ALVEAFTALLAAPLGPYDVAHVAWEIERIDLGLAGRKQDQYAATFGGTNFIEFHSGDR
                     VIVNPLRVASPVLNELETSMVVCFTGVSRRSEAIIIEQQHGMTNVPDDVPAGGVIESL
                     HQLKRDAVEMKEALLRGQIPRMADILNRSWAAKKRTASGISTGRIEALYELAFANGAI
                     GGKVSGAGGGGFMMFIVPPERRIAVVRALNRAGGTASGVHLTGEGAESWT"
     gene            complement(1073..2308)
                     /locus_tag="VGC82_09120"
     CDS             complement(1073..2308)
                     /locus_tag="VGC82_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEY0423467.1"
                     /translation="MMSPRGATVTQCAVLVGGLGTRLGALTATTPKPILPCGGRPFLG
                     WLLREFVRYGVTDFLLLTGHLSDEIERAAADIQATLPRPARIALSQEPERAGTGGAVF
                     HAREQLQARFLLCNGDSLFDFNLANLLADAAADGPDVIGRIVLRRLEDASRYGVVDLE
                     QDCVTAFHERPPAGTGGTINGGIYLFGHSLMEHLRPACSLEADVLPALARAGSLRGTL
                     GQGYFRDIGLPDDFARAQTEIPALLRRKALFLDRDGVINIDHGYIGSRDRFEWVDGAL
                     EAIRHATEAGWHVFIVTNQSGVARGLFDEAAVHDLLDWIGDQARAAGGTIDDVRYCPF
                     HPDAALPAYRQMHPWRKPLPGMLLDLIQAWDLDPSRTVMIGDQATDMQAAEAAGITGY
                     LFRDGNLLSFLRPILDKHA"
BASE COUNT          443 a          853 c          753 g          374 t
ORIGIN      
        1 ccgtccacga ttccgcgccc tcgccagtca gatgcacgcc gctcgcggtt ccgccagcgc
       61 ggttcaacgc ccgcaccacc gcgatgcgcc gctccggcgg cacgatgaac atcatgaacc
      121 cgccgccgcc agcaccggac accttcccgc cgatcgcccc attggcgaag gcaagttcgt
      181 acagcgcctc gatccgcccc gtgctgatac cggacgcggt tcgtttcttg gctgcccagg
      241 agcggttcag gatgtcagcc atccgcggga tctggccgcg caatagcgcc tctttcatct
      301 ccacggcatc gcgcttcaac tggtgcaggc tctcgatcac gccgccggcc ggcacatcgt
      361 cagggacgtt cgtcatgcca tgctgctgct cgatgatgat ggcttccgac cggcggctga
      421 ccccggtgaa acacaccacc atggaggtct ccagttcatt caggacaggg gaagcgacac
      481 gcagcgggtt gacgatgacc cggtccccgc tgtggaactc gatgaaattg gtgccgccga
      541 acgtcgcggc atactgatcc tgcttgcggc cagccaggcc aagatcgatt cgttcgatct
      601 cccacgcaac gtgtgccacg tcgtacggtc ccaacggtgc cgccagcagc gcggtaaatg
      661 cctccaccag ggccaccacc agggccgagg acgacccaag ccccgacccg ggcggcgcat
      721 cgacgtagga tgttatgcgc atcggcctga tcttggcgtc cccaaactcc gtcagcatgc
      781 ggcgcgcgac gcccgcatgc agtccgagcc ggccctgcga tagcgcgtcc aggtcgagtg
      841 gaaaatcttc ctcgatcgcc aggtccgggg caacgaactg aacgcggccg ttgtcggacg
      901 gcgcgatgaa cgcataggca tagcggtcga tcgtcgtgtt cagcaccgcg ccgccgaact
      961 ggtcgcaata ggggctcaag tcggtgccac cgccagcaag accgagccga agcggaacac
     1021 gcgctcggat tgtgggctgc cgggaccgca acggcatctc ctgattccgt gatcaggcgt
     1081 gcttatccag gatgggtctc aggaaagaaa gaagattccc gtcgcggaat aaatagccgg
     1141 tgatgccagc cgcctccgcg gcctgcatgt ccgtcgcctg atccccgatc atcaccgttc
     1201 gggaaggatc gaggtcccac gcctggatca ggtccagcag catgcccggc agcggcttgc
     1261 gccacggatg catctgccgg taggccggca acgccgcatc cggatggaac gggcaataac
     1321 gaacgtcgtc gatcgtgccg ccggccgccc tcgcctgatc gccgatccag tccagcaggt
     1381 catgcacagc ggcctcgtcg aacaagcccc tggcgacgcc ggactggttg gtgacgatga
     1441 acacatgcca cccggcctcg gtcgcgtggc ggatcgcctc cagcgcacca tcgacccact
     1501 cgaaccggtc gcgcgaaccg atatagccgt ggtcgatgtt gatgacgccg tcgcggtcca
     1561 ggaacaaagc cttgcgccgc agcaaggccg ggatctcggt ctgcgcgcgc gcgaaatcgt
     1621 ccggcaagcc gatgtcgcgg aaatacccct ggcccaaggt tccccgcagc gacccggctc
     1681 gggccaatgc cggcagcacg tccgcctcca gcgagcaggc cgggcgcaaa tgctccatca
     1741 gcgagtggcc gaacagatag atgccgccgt tgatcgtgcc gccggtgccg gccggaggac
     1801 gctcgtggaa tgccgtgacg caatcctgct cgagatccac cacgccatac cgcgaggcgt
     1861 cttccagccg gcgcagcacg atccgcccga tcacgtccgg cccgtcggca gccgcatccg
     1921 ccagcaggtt cgccaggttg aagtcgaaca gcgagtcacc gttgcacagc aggaaacgcg
     1981 cttgcagttg ctcccgcgca tgaaacaccg cccccccggt gcccgctcgc tctggctcct
     2041 gcgacagcgc gatccgggcg ggacgcggca aggtcgcttg gatatcggcg gcggcacgct
     2101 cgatctcgtc cgatagatgg ccggtcagca gcaggaaatc ggtcacgccg tagcgcacga
     2161 actcgcgcag cagccaaccc aggaacggcc gaccgccgca gggcaggatc ggtttcggcg
     2221 tggtcgcggt cagtgcccct aaacgcgttc ccaggccgcc aaccagcacc gcgcattgcg
     2281 taacggtggc accccggggg gacatcatag cggggagatc tcggtttccg cggcgggttc
     2341 gcggccggcg agcaccttgt ccacgcgtcg gccgtccatt tccaggactt caaagcgcca
     2401 gccgccgaac acgatgcggt ctc
//
LOCUS       DASWQN010000545         2946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2164239, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000545 DASWQN010000000
VERSION     DASWQN010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164239"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGC82_09125"
     CDS             <1..234
                     /locus_tag="VGC82_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423468.1"
                     /translation="RVPPGVAGTIAPVTSVMVEETADALAKAERAGLAVLVRTSGLPD
                     AAKIGPIGLTAPLIIWLVDTWLSFGIPVAGAFG"
     gene            complement(434..1699)
                     /locus_tag="VGC82_09130"
     CDS             complement(434..1699)
                     /locus_tag="VGC82_09130"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEY0423469.1"
                     /translation="MKRLAACLLLGVLAACVAQRDPVLDADAALASGMMPERLASGAV
                     CRVGPDGGALIADRGIGGTGAPPAARQIADRGIGGTGIVGFITGFASICVDGLEVRYD
                     GSALVDIDGTAAPASALRVGQVVVIQANGPAGAPVARTITVRREVAGQIESIDLASGT
                     LTVAGQPVSVPTSIWGAKRARLGAWIAVSGMRRADGTIVASRLDNAPAGSFMARGQIQ
                     REGGLVKLGNLTLRGSAATSLATGQFITASGHYAAGQGQVDSVTSDTLSQDPRAFFGA
                     SVKHLVVQGLVRITNGAVALNGLKATAGSSLNARSGSSGIAIVSLERKPDGTYTAVGL
                     RYTDYRGPAARAGRAVGAPVGSSTPARTTCGHRRRPRRMALVAGCPIWRHQPCPMMPT
                     RVRQLRGWPRRRETRQRRHRQPQRQQRWR"
     gene            complement(1696..2553)
                     /locus_tag="VGC82_09135"
     CDS             complement(1696..2553)
                     /locus_tag="VGC82_09135"
                     /inference="COORDINATES: protein motif:HMM:NF039473.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HEY0423470.1"
                     /translation="MSPPDRFRPLQPAALLKAARGLMRPLVRLMLRSGLTFPVLAETL
                     RRLFVEVAVNDILIDPNARTDSRISLLTGVHRKEIKRLRGIPADAAAVPDVVTLVSQI
                     VGRWVGTAAFLDGAGRPLPLVRLGSAIPGGAPSFDALVESVTSDIRPRAVLDDLLSHG
                     VVYMDSEGRVQLHADAFIPRPGGEEQLFYFGRNLHDHVAAAVSNISAPEAPPFLDRTV
                     HYDGLTPAQALELQDYARAAAMQVLLDVNRRAIELTASTPEVTQTRRVNFGVYLFDEN
                     EHPGAGAAS"
BASE COUNT          535 a          989 c          919 g          503 t
ORIGIN      
        1 cgtgtgccgc caggcgtcgc ggggacgatc gctcccgtca ccagcgtgat ggtggaagag
       61 acagcagacg cactggctaa ggccgaacgc gctggactcg cggtccttgt tcgaacaagc
      121 ggcttgccgg atgccgccaa aattggcccc attggcctaa cagcgccgct gatcatctgg
      181 ctggtcgaca cttggctgtc gttcgggatc ccggttgccg gcgcattcgg ctgaatcggt
      241 ggtgccgaga cggttggggc gaccattggg gacatcggtg gctgcaaaac cccggggaca
      301 agcgataccg ccggcccctg aaccgacgct gccagcggga tcgccaccaa cgccatcacc
      361 ggcgccaggg tgattttcaa agtcgaagcc ggagattccg ccgatgccga tcccgaggtt
      421 gccgatcccg aggttaccgc caacgttgtt gtcgctgagg ctgccgatgc ctccgctgtc
      481 gcgtctcccg acgtcggggc cagccgcgca attggcgtac ccgggtcggc atcatcgggc
      541 acggctggtg gcgccatatc gggcaacccg ccaccagcgc catccgtctg gggcgcctcc
      601 ggtgtccgca cgtcgtgcgc gctggcgttg aggatccgac cggtgctccc accgcccgtc
      661 ctgccctggc ggcgggaccg cgataatcgg tgtaacgcag cccgacggcg gtataggtgc
      721 cgtccggctt gcgctccagg gatacgatgg cgatcccgct ggaacccgac cgtgcgttca
      781 ggctcgatcc ggccgttgcc ttgaggccgt tcagcgcaac cgcgccgttt gttattcgga
      841 ccaaaccctg gaccaccaga tgcttgaccg aggcaccaaa aaacgcccga gggtcctgcg
      901 acaatgtgtc ggaggttacg gagtccacct gtccctgacc cgcggcataa tggcccgagg
      961 ccgtgatgaa ctggccagtc gccaaacttg tggccgccga accgcggagc gtcaggttgc
     1021 ccagcttgac cagtccgccc tcacgctgga tctgcccgcg ggccatgaat gacccggccg
     1081 gcgcgttgtc caagcggctg gcgacaatcg tgccgtcggc tcggcgcatg ccgctgaccg
     1141 cgatccaggc gcccaagcgc gcccgtttgg cgccccagat cgaggttggg accgagaccg
     1201 gttgtccggc aaccgtcaga gtgccggatg cgaggtcgat cgattcaatc tggcctgcca
     1261 cttcccgccg caccgtgatc gtccttgcga cgggtgcgcc cgcaggcccg tttgcctgga
     1321 taaccaccac ctgcccaacc ctcagcgccg aagccggagc cgctgtccca tcgatatcga
     1381 ccaaagccga accgtcatat cgcacttcca ggccatcgac gcagatcgag gcgaatccag
     1441 taatgaaccc gacaattccg gtgccgccga tcccacggtc cgcgatctgg cgggctgccg
     1501 gtggagcgcc ggtgccgccg attcctcggt cggcaatcag cgccccgcca tccgggccaa
     1561 cgcggcatac cgcgccgctg gccaggcgtt cgggcatcat tcccgatgcc agcgccgcat
     1621 cggcgtcgag gacgggatct cgctgcgcaa cgcaggccgc caaaaccccg agcaagagac
     1681 acgccgcgag acgcttcatg atgcggcccc ggcacccgga tgttcgttct catcaaacag
     1741 gtaaacgccg aagttgacac gccgtgtctg ggtcacctcc ggcgtggatg ccgtcagttc
     1801 gatggcccgc cggttgacgt cgagcagcac ctgcattgcc gctgcccgcg cgtagtcctg
     1861 gagttccagt gcctgcgccg gcgtcagacc gtcatagtgc acggtgcggt ccagaaacgg
     1921 cggtgcctcg ggtgcgctta tgttgctcac cgccgcggcg acatggtcat gcaggttgcg
     1981 tccgaagtag aacaattgtt cctcgccccc gggacgggga atgaacgcat cggcatgtag
     2041 ctgaacgcgg ccctcggagt ccatgtaaac gacaccgtgg ctcagcaggt catccagcac
     2101 cgcccgcggc cggatgtcgc tagtgacaga ttccaccagt gcatcgaatg acggcgcacc
     2161 gcccggaatg gcggaaccga ggcgaacgag cggcaatggg cgccctgccc cgtcgaggaa
     2221 cgccgcggtg ccgacccagc ggccgacaat ttggctgacc aaggtgacga cgtccggtac
     2281 ggccgccgca tccgctggta ttcctcgcag gcgctttatt tccttgcggt gcacccccgt
     2341 taacaggcta atccggctat cggtccgcgc gttcggatcg atcagaatgt cattgaccgc
     2401 gacttctacg aataagcggc gcagcgtctc cgccaaaacc gggaaggtca acccgctgcg
     2461 caacatcaat cggaccaaag gtcgcatgag cccgcgcgcc gccttaagca gcgccgctgg
     2521 ttgaagcggt cggaagcggt cgggcggcga catgcgtgct gataatcctt acttgaaagg
     2581 ttatagcacg gcttaatgtg ggaaaaatac ccacatcacg acaaaattcg attgacgtgg
     2641 gaaattttcc cacctacggt ttgaatatcg aagcggggtg ctcggtcgcg ctttcggacg
     2701 ccggcaaaag cgaccttctg accgctttga aggttcggga tcaatccgag ccgccaccgc
     2761 ccttgtacgc aactttcaaa cgaggccccg gacatgtcat acgtcgccgc ctccgcgctc
     2821 agaacatccc gtgcgccggt caacccgcag gtcagccgcg ccgctcggcc agtatttcat
     2881 cggccggccg atttgcggga cgacgccgat ttacgggatg atggggatga cacgcccgat
     2941 cgagac
//
LOCUS       DASWQN010000546         3903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1372917, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000546 DASWQN010000000
VERSION     DASWQN010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372917"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VGC82_09140"
     CDS             <1..1263
                     /locus_tag="VGC82_09140"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0423471.1"
                     /translation="FLVLAAAVSLAGRAVAELMTAFFPEGVPGYDREDGVTVQTRLHP
                     EQMPIGVHEGAFEFFPRIDESVGYSSNAFSGSRRRGSWEIVTNPSLAIGSAWSRDAFG
                     AMFSFQDTQYLALPSQNRTDATASVGGRVDIGEDRLTLAAAHVTQHEDRSQIGSIAAD
                     RPIAIQLDDIRASYAIDRGRWSFVPSMEASNWTYGGTTILGVPASQSYRDRVVLQGGL
                     TVRYEFAPLRSLVLVARAIEQDYTRTPVGQVGPNSTSYQMLAGIDYDDDSVWRYRLLV
                     GGETRQFASPSYPRQNTLIAEGGIGWSPSGMTTINATVIRGTADAAQEGVSGLIYSAA
                     RLTIDHEYLRDLLLKASAGLQQADFFQGGHQSGTSFGVGATWVLNRNARLSFTYDQTD
                     LHGSPVSAQALGTGYSRGIGLVTLRLML"
     gene            complement(1408..2424)
                     /locus_tag="VGC82_09145"
     CDS             complement(1408..2424)
                     /locus_tag="VGC82_09145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0423472.1"
                     /translation="MNARLVAGAAGPLDGSYDVDIVILALQRTEETLAAIASASSQIG
                     VSAHIFVLDQGSCSQALGRLADAVRGRRDITLLAAEQNLGVAGGRNLISNLGHGRVIV
                     ALDNDAEFATTDTVARLVAALDAEPRLGAIACRIVTYADGSDDLTSWGYPANLLPCAG
                     ESFDAVTYVGAGHAILRTAWQQAGGYDSRLFFCWEEFDFCLRAIALGWRIRYRGDIVI
                     RHKVSPEQRVGWNANRWSYFVRNRLYIERKLDRGWIALTPRIAGYLLKGSRNGLLVQT
                     VRAILAAADMAPRSTARPMPRAGLSYLTRNDRAHRGSLLSRLLREALGRIGTRSNAVP
                     GEKR"
     gene            complement(2421..3443)
                     /locus_tag="VGC82_09150"
     CDS             complement(2421..3443)
                     /locus_tag="VGC82_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0423473.1"
                     /translation="MSETLEWLPSRTIDRGWQSDRSLAKQFGLETNQPRVAVLVPCRN
                     EAAAIGKVVSDFQRALPDAIVYVYDNNSTDRTMLEARAAGAVVRRETLQGKGHVVRRM
                     FADVDADIYILVDGDDTYDASAAPGMLNMLVERQLDMVCGARYGTASAAYRPGHRMGN
                     ALLTGLVSAVFGGGITDMLSGYRVFSRRFVKSFSALSSGFETETEFTIHALALHMPVQ
                     EVRVIYRNRAAGTASKLNTLADGMRILRAIVTLIQQERPFQVFSCAALILCVLGTSLG
                     VPVVMEFLQIGLVPRLPTALLSTGLMLLAFLSLTCGLILDAVSRGRKENKRLAYLAIP
                     ALEAYE"
     gene            complement(3459..>3903)
                     /locus_tag="VGC82_09155"
     CDS             complement(3459..>3903)
                     /locus_tag="VGC82_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY0423474.1"
                     /translation="FVMLKFRTMHDAPAAPNTCPQAIRHDPRVTPIGAFLRRTSFDEF
                     PQLLNVLAGSMSLVGPRPHAPGTCAGGRPFEAVSHRYAARHCVKPGMTGLAQIRGWRG
                     ETDTEEKLLRRIDSDLEYIAIWSLGRDLMILCRTIVTVLRMPNAY"
BASE COUNT          744 a         1175 c         1281 g          703 t
ORIGIN      
        1 tttcttgttc tcgccgcggc ggtcagcctg gccggccgag ccgtggccga actgatgacg
       61 gcgttttttc cggagggggt tccgggctac gatcgcgagg acggtgtcac ggtgcaaacg
      121 cgtttgcacc ccgaacagat gccgatcggg gtgcatgagg gtgccttcga attctttccc
      181 agaatcgacg agagcgtcgg ctatagcagc aatgcgtttt caggatctcg tcgccgagga
      241 agttgggaga tcgtgaccaa cccatcgctg gcgatcgggt ccgcttggtc gcgtgacgcc
      301 ttcggggcga tgttttcgtt tcaggacact caatatttag ccttacccag ccaaaaccgc
      361 acggacgcga cggcatccgt gggcgggagg gtcgatatcg gcgaagacag gctgacgctg
      421 gcggcggccc atgtaacaca gcacgaggac cgtagccaga tcggcagcat tgccgccgac
      481 cgcccgatcg cgatccaact cgacgacatt cgtgcatcct atgcgatcga ccgcggccgc
      541 tggagcttcg ttccgagcat ggaggcgtcg aactggactt atggcgggac gacgatactc
      601 ggggttccag ccagtcagtc ataccgcgac agggtcgttc tgcagggcgg cctcacggtg
      661 cgttatgaat tcgcgccgct gcgcagcctg gtgttggtgg cccgagcaat agagcaggac
      721 tatacgcgaa cgccggttgg tcaggttggc ccgaattcta ccagctacca gatgctcgcg
      781 gggatcgatt acgacgacga ctcagtttgg cgttaccggc tgcttgtcgg cggcgagact
      841 cgccaattcg ccagtccgtc gtatccgcgg cagaatacgc tgatcgccga gggtgggatc
      901 ggatggtcac cctccggcat gacgacgata aacgcgacgg tcatccgggg gacggcggat
      961 gccgcgcagg agggggtctc cggactgatc tactccgccg ctcggctgac gatcgatcat
     1021 gaatatctgc gcgacttgct gctgaaggca tcggcgggtt tgcaacaggc cgatttcttc
     1081 caaggtgggc accaaagtgg cacaagcttc ggcgtgggcg cgacgtgggt gctgaatcgc
     1141 aacgcccgac tgtctttcac ctacgatcaa accgatttgc atggttcgcc tgtatcggcg
     1201 caggcgttgg ggaccgggta tagtcgcggg ataggcctgg tgacactgcg actgatgctt
     1261 tgaggacagg tgctttgaga ccgggcgctt tgagaccggg cgctttgacg acaggcgcgt
     1321 aacgcgcaca ggagggagcc gggtgttgtt cgtgcctgaa gcggccgttg cgcctggtac
     1381 ggtgctagtc ggctgacggg gccgcgttca tcgcttttct cccgggacgg catttgaccg
     1441 agtgccgatc cggccaagcg cctcccgcag gaggcgggat aacaacgatc cgcggtgggc
     1501 gcggtcattc ctggtcaagt aggacaagcc ggctcgcggc atcgggcgcg ctgtcgaacg
     1561 gggtgccata tcggcggcag ccaggatcgc ccgcacggtc tggaccaaca gcccgttgcg
     1621 cgagcctttc aacaggtaac ccgcgattcg gggtgtcagc gcgatccagc ctcgatccag
     1681 cttacgttcg atgtaaagcc ggttgcgcac gaagtaggac cagcgattgg cgttccagcc
     1741 aaccctctgt tcgggcgaaa ccttatggcg gatgacaata tcgccgcggt agcggatgcg
     1801 ccagccaagc gcgatggcgc gtaggcagaa gtcgaattcc tcccagcaga agaacagccg
     1861 ggagtcgtag cctccggctt gctgccaggc cgttcgcagg atggcgtggc cggcgccgac
     1921 ataagttacg gcatcgaagc tttcgccggc gcaaggtaac aggttggcgg gataacccca
     1981 ggacgtcagg tcatcgcttc cgtcggcata ggtaacgatg cggcaggcga tcgccccaag
     2041 acgcggttcg gcatccagcg ctgcgaccag acgggcgacg gtatcggtgg tggcgaactc
     2101 ggcgtcgttg tccagcgcga cgatgacccg gccgtgcccg aggttcgaaa tcaggttacg
     2161 gccgcccgcc accccaagat tctgttcggc cgccagcaga gtgatatcgc ggcgacctcg
     2221 caccgcatcg gccaaacggc ctagtgcctg ggagcacgat ccttgatcca gcacgaagat
     2281 atgggccgag acaccgatct ggctgcttgc cgaagcaatg gctgccagcg tttcttcggt
     2341 ccgctgaaga gccaggatga cgatgtccac gtcatagctc ccatccaaag ggccagcggc
     2401 accggcgacc aaacgcgcgt tcactcgtac gcctccagtg cggggatcgc cagataggcc
     2461 aggcgcttgt tttctttccg gccgcgggac accgcgtcca ggatcaaccc gcaggtcagc
     2521 gacaggaaag ccagcagcat caatccggtt gacagcagcg ccgtcggcaa gcgcggcacg
     2581 aggccgatct gcaggaactc cattaccacc ggaacgccga ggctggtgcc gagaacgcac
     2641 aggatcagcg cggcgcagga aaagacctga aacggccgct cctgctgaat cagcgtcacg
     2701 atcgctcgca ggatacgcat gccatcggcc aacgtattga gtttcgaagc ggttccggca
     2761 gcccgattgc ggtaaatcac ccgtacctct tgcaccggca tgtgcagggc gagggcatga
     2821 atggtgaatt ccgtttccgt ctcgaagccg gaggacaaag ctgagaacga cttgacgaac
     2881 cggcggctga acacccggta gcccgacagc atatcggtaa tcccgccacc gaataccgcc
     2941 gaaacgaggc ccgtcagcag ggcattgccc attcgatggc cgggacgata ggcagcggat
     3001 gcagtgccgt agcgggcccc acagaccata tccagttgcc gctcgaccag catgttcagc
     3061 atacccggcg cggccgaggc gtcgtaggta tcgtccccgt ccaccaggat gtagatgtcg
     3121 gcatcgacat cggcgaacat gcgccggacc acatggcctt tgccttgcag cgtttcccgc
     3181 cgcacgaccg cacccgccgc tcttgcctcc agcatggtac ggtcggtgga gttgttgtcg
     3241 taaacataga cgatcgcgtc gggcagggcc cgctggaagt cggacacgac cttgccgatc
     3301 gcggccgctt cattccggca tgggaccagc accgccacgc gcggctggtt agtttccaga
     3361 ccgaattgtt tggcaaggga tcgatccgac tgccagcctc ggtcaattgt tctcgaaggg
     3421 agccattcca gagtctcgga catcgtgatc gctcccaact agtaggcgtt gggcatgcgc
     3481 agcaccgtga cgatggtgcg gcagaggatc atcaggtcgc ggccgaggct ccagatggcg
     3541 atgtattcca ggtcgctgtc gatccgacgg agcagttttt cttcggtgtc ggtttcgccc
     3601 cgccagccgc ggatttgggc cagcccggtc ataccgggtt tgacgcagtg acgggcggcg
     3661 tagcggtgcg acaccgcctc gaacggacgc ccgccagcac aggtgccggg tgcatggggg
     3721 cgcggtccga ccagcgacat cgatcctgcc agcacgttga gcaactgggg gaattcgtca
     3781 aaggaggtgc ggcgcaggaa ggccccgatc ggagtgacac gcgggtcgtg ccggatggcc
     3841 tgcgggcagg tgttcggtgc cgccggcgca tcatgcatgg tgcggaattt cagcatcacg
     3901 aag
//
LOCUS       DASWQN010000547         5680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2296247, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000547 DASWQN010000000
VERSION     DASWQN010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2296247"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VGC82_09160"
     CDS             complement(<1..185)
                     /locus_tag="VGC82_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423475.1"
                     /translation="MKMWMAVLGAAIHVLVSVLKSHGWPAQGRPVHKINRLEAHRRRN
                     LILTSPSVPSVAAARAR"
     gene            1099..4425
                     /locus_tag="VGC82_09165"
     CDS             1099..4425
                     /locus_tag="VGC82_09165"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY0423476.1"
                     /translation="MNKAEAVRVAGPGLLPLAIADWVCGTGRSRTVFIAPDDVALDAL
                     HAALPVFLPDHHVLALPQWDSLPYDRARPSRGVTGRRVASLSWLIDPQDRPAVVLTTP
                     EALPQRIPPAERLRERAVRLAVGDALDPEWLRATLSAFGYTFEEWVDAPGEAAIRSGT
                     VDIYPAGQADPVRLELDSGRVVAIRTFNPLTQRGADDSGPVVLLPASEAFTVPGEGDG
                     AETDDIQKGRVAERLLPEGPLATLFDLMPNVAVGLFENTAERVADWLALVRDGYSVAL
                     TSYRAGQAVPPPPEELYMPEADVAAALASQDCTHFIVGNEKVEIALPPAALSRRAAMC
                     LEKGDAVIICSSHPSQRIRPILARRLGIPAEDIRRLETWQEAVSLPAGLVGVITLPLR
                     EGFRLPGRVILAERDTAQEAIDDADAVQRLSAALRLGDMVVDPERGLARLVGLAMEDQ
                     AGAACECLSLEFMGGQRLLLPAFEAGRIWRYGSTGSVSADRLDGTSWSERRHQAERAI
                     HAAAAGLVERLQARERMRAPVIEPCPAYHRFVRRMPFAPSADQVRAVRAVRDDLAAGR
                     PMHRLVCGDVGFGKTEIALHAAALAAFAGRQVAVIAPTTVLAHQHLETFRRRLRGFDL
                     TIEPLIRGTRSSAAKAVLRGVRTGEVNILVGTHSVLAARFHDLGLAVIDEEQRFGEAQ
                     KQALRTLHEGGHALVMTATPLPRSLQAALLGLVDVSLLTQPPAPRLPVRGLVLPFDPV
                     VVRTALLREARRGGQSFVICPRIEDIAPMESRLRALVPELSLTAIHGRMGADVLHEAM
                     MDFSAGDRDVLLATNIIEAGLDLPNANTMLVWRPDRFGLAQLHQLRGRVGRGRVRASA
                     YLLTDPGHPPSEAARKRLDTIAGLDSLGGGFGISLADLDQRGAGDLLGEEQAGHMVII
                     GTERYQDVLRRALAAARGDPVRDEWTPELVLDLPAYAPADFIPEPDIRLDIYRRLARL
                     ETFTALDEATEDIADRFGAIPPPVEALLGLTRLRLLCLDRGIAAVHAGPAGVALTPRP
                     GRMEALWRIEGSRRVKERVILPIMTPSPTNRLRDVMAAVGSVALCDEETKDFQGVPQI
                     DRHVWRIVSTRAGWNPSGAT"
     gene            4489..5049
                     /locus_tag="VGC82_09170"
     CDS             4489..5049
                     /locus_tag="VGC82_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY0423477.1"
                     /translation="MPNTEWLPLSRLPTFDAASGDVTAIIETPKGSPNKYDYDPGCGA
                     FKLAGVMPEGTTFPYDFGFIPSTLAEDGDPLDVLVFLDAPVVVGCVLTVRMLGVIEAE
                     QRKKGQDWIRNDRLLAVATHAHTHDHVRTLDDLRPHLLDEIEAFFSHYNEMKGSEFKP
                     IKRGGPESARNLIQQAEGNFRKQNDA"
BASE COUNT          988 a         1800 c         1864 g         1028 t
ORIGIN      
        1 aaacgggcgc gggcagcagc aaccgagggc acgcttggtg aggtcagaat cagatttcga
       61 cgacgatgcg cttcgagccg gttgatcttg tgtactggcc ggccttgtgc cggccagcca
      121 tgacttttta gtactgaaac aaggacgtgg atggccgcgc cgagcacggc catccacatc
      181 ttcatcttgc gtcctggcta ccccgggcag tcggccacca cggagagatc gttagccctt
      241 gcgggcccgt ccctcgccat gacgggatcg cagaccaccg ggggtggcta actccgggct
      301 ccggtctaac aatcttccgc ctcttccagc tgctggctct tgcttttacc cggagcaggc
      361 gtggaaccgg gcgttccacc actgacggtc agcggttcat ccggtccggg cgctttttcc
      421 ttgccgaacg cgccttcgac gttgccgccg ttgtcttgct gcttactccg agccattgct
      481 tgtcctcatg tgccgtagtg tcagtaattc cagcgacgtc acgcacgacg gacgcgacgc
      541 tgaactaaaa gcgcgagggg cgaggcggtt gcgggctaag caatccctac gcggcttcgt
      601 gaccatggac caggtgcggc ggaacccagt ggccggaacc cagtggccgg aacccagtgg
      661 ccggaaccaa gtgcggcgga accgagtgcg gcggatcgaa gcgcgccagg cgtcacatcc
      721 catgcgatcc gaaccgccgc cgcgttacga aactcgggcc gccggcgatt taccccagcc
      781 tgcggcaacc gggtgcctcc ggcgacgttg cccgtcagcc aaaaccacag gggtccaaaa
      841 tgagtgatga tgggtcgatt gcttcgggat tgggccaggc taccggcaga ccggcagcgc
      901 agccgaaatt gtatagtgcg gccggcgaac cgctgcgtgg ctcgcgtccc gagcgcagtc
      961 agacggatca gttggcggcg ttcgtgcagg agcaacctgt tacggctgca ctggctgctt
     1021 tggtcatcgg atatttcctc gggaaaataa cctgacagct acgcccgcga aaaaggttgt
     1081 tgtcgccaaa cgtttcccat gaacaaggca gaagcggttc gcgtcgcggg gccaggtctg
     1141 ttgccgttgg cgatcgccga ctgggtgtgc ggaacgggtc gttcgagaac agtctttatc
     1201 gctcctgacg acgtggcact ggatgcgctg cacgcggcgc tgccggtgtt tctgccggac
     1261 caccacgtcc ttgccctgcc gcaatgggac tccctgccat atgatcgcgc ccggccctcc
     1321 cgcggcgtaa caggccggcg ggtcgcgtcg ctgtcctggc tgatcgatcc gcaggaccgg
     1381 ccggcggtcg tcctgacgac gcccgaggcg ctgccacagc gcataccgcc agcggagcga
     1441 ctgagagagc gggcggtaag gctggcggtg ggcgacgcac tcgatcccga atggctgcgc
     1501 gccacgctca gcgcattcgg atacacgttc gaggaatggg tggacgcgcc gggggaggcc
     1561 gcgattcgat ccgggacggt ggacatctat ccggcgggac aagccgatcc cgtgcgcctg
     1621 gaactggata gcggacgcgt cgtcgcgatc cggaccttta acccgctgac acagcgcggt
     1681 gccgacgact caggcccggt tgtcctgctg ccggcgagcg aggccttcac ggttcccggt
     1741 gaaggggatg gcgccgagac ggatgacatc cagaaggggc gggtcgccga acgcctgctg
     1801 ccggaaggac cgttggcgac cctgttcgac ctgatgccga acgtcgccgt cgggttattt
     1861 gaaaataccg cggagcgggt cgcggactgg ctggccctgg tgcgcgacgg ctacagcgtt
     1921 gccctcacct cctaccgcgc cggccaggct gtcccgccgc cgccggaaga actctacatg
     1981 ccggaggcgg atgtcgccgc cgccctggcg agccaggact gcacgcactt catcgtgggc
     2041 aatgaaaaag tggaaatcgc gttgccccct gccgcactat cgcgccgggc agccatgtgc
     2101 ctggaaaagg gcgacgctgt catcatctgc tcgagccatc cctcgcaacg catccggccc
     2161 attctggcgc gacggctggg catccccgcc gaggatatac gccgcctcga gacatggcag
     2221 gaagccgttt cgctgcccgc cggcttggtc ggagtgataa ccctgcccct gcgcgagggc
     2281 ttccggttgc ccgggcgggt catcctcgcc gaacgggata cggctcagga agcaatcgac
     2341 gatgcggatg ccgtgcagcg tctatccgct gccttgcggc taggtgacat ggtggtggat
     2401 ccggaacgcg gcctcgcccg gttggtcggg ttggcgatgg aggaccaggc cggtgccgca
     2461 tgcgaatgcc tgtcgctgga gttcatgggc gggcagcgtc tgctgctgcc ggcgttcgag
     2521 gccgggcgta tctggcgcta tggttcgact ggctcggtct ccgccgaccg gctggacggg
     2581 acgtcctggt cggagcggcg ccaccaggcg gagcgcgcca tccacgccgc cgccgccgga
     2641 ctggtcgagc gtcttcaggc tcgtgagcgc atgcgggcgc cggttatcga accgtgcccc
     2701 gcctatcatc gcttcgtccg ccgcatgccg ttcgcgccca gcgcggatca ggtccgggcc
     2761 gtccgcgcgg ttcgcgacga ccttgcggcg ggccgcccga tgcatcggct ggtttgcggg
     2821 gacgtcggtt tcggcaagac cgaaatcgcc ctgcatgccg ccgctctggc cgctttcgcg
     2881 gggcgccagg tggctgtcat cgcgccgacc accgttctcg cgcaccagca tctggaaacg
     2941 tttcgccggc gtctgagagg cttcgacctg acaatcgagc cgctgatccg gggcacccgc
     3001 tccagcgccg cgaaagcggt gctgcggggt gtcaggacgg gcgaggtcaa catcctcgtc
     3061 gggacccatt cggtgctggc ggccaggttc cacgacctgg gtctggcggt catcgatgaa
     3121 gagcaacgct tcggtgaagc gcagaaacag gcattgcgca cgctccacga gggcgggcac
     3181 gcgctggtca tgacggcgac acctctgccg cgcagcctgc aggccgcgct gctgggcctg
     3241 gtcgatgtca gcctgttgac gcagccgccc gccccgcgcc tgccggtgcg cggacttgtg
     3301 ctgccgttcg atccggtggt cgttcgcacg gccctgctgc gggaggctcg ccgtggtggg
     3361 cagagctttg tcatctgtcc ccgcatcgag gacatcgcgc cgatggaaag ccggttgcgg
     3421 gcgctggtgc cggagttgtc gttgacggcg atccacgggc gcatgggggc cgatgtgctg
     3481 catgaagcga tgatggattt ttccgccggc gaccgcgacg tgctgctggc caccaacatc
     3541 atcgaggcgg ggctggacct gccgaacgcc aacacgatgc tggtatggcg gccagaccga
     3601 tttggcctgg cgcagttgca ccagctgcgc ggacgggtgg ggcgcggacg ggtgcgggcg
     3661 tccgcatacc ttctaacgga tcccggtcat ccgccgtccg aagccgcgcg gaagcgactg
     3721 gacaccatcg cggggctcga tagcctcggc ggcggcttcg ggatcagcct ggcggacctg
     3781 gatcagcgcg gcgccggcga cttgctgggc gaggaacagg ccgggcacat ggtcatcatc
     3841 ggaaccgagc gttaccagga cgtcctgcgg cgcgcgctgg ccgccgcccg tggcgatccg
     3901 gtccgcgacg aatggacacc ggaactcgtg ctggatcttc cggcctacgc gcccgcggac
     3961 tttatccccg aaccggatat ccgcctcgat atctaccgtc gcttggctcg cctggaaacc
     4021 ttcacggccc tggatgaggc gacggaggat atcgcggacc ggttcggtgc gattccgccg
     4081 ccggtcgagg cattgctggg cctgacgcgg ctgcggctac tatgcctgga tcgtggaatc
     4141 gccgccgttc acgccggtcc ggccggcgtt gccctgacgc ctcggcccgg caggatggag
     4201 gcgctgtggc gcatcgaggg ttcgcgacga gtcaaagaac gcgtcatcct gccgataatg
     4261 accccatcgc cgacaaaccg tcttcgcgac gtcatggcag ccgtcgggtc ggtcgccctt
     4321 tgcgatgaag aaaccaagga tttccaaggt gttccgcaaa ttgaccgaca tgtgtggcgg
     4381 atcgtttcta cgcgcgccgg ttggaatccg tcgggcgcaa cttgaacgcg cgatcggagt
     4441 tatccactga ttggcggatc ccggccgcgt caatcccagg agggcctgtt gccgaacacc
     4501 gaatggcttc cactatcgcg cctgccgact ttcgacgcgg cttcgggcga cgtgacggcc
     4561 atcatcgaga caccgaaggg tagcccgaac aaatacgact atgatccggg ctgcggcgct
     4621 ttcaaactgg cgggggtcat gccggagggc acgaccttcc cctacgattt cgggttcatc
     4681 ccctccaccc tggccgagga cggcgaccca cttgacgtct tggtgtttct tgacgctccg
     4741 gtcgtcgtcg gctgcgtcct gacggtgcgc atgcttggcg tcatcgaagc ggagcagcgc
     4801 aagaaagggc aagactggat caggaacgat cggctccttg ccgtggccac ccatgcgcat
     4861 acgcatgatc acgtccgaac gcttgacgac ctgcgtccac accttctgga tgagatcgag
     4921 gcattcttta gccactacaa cgaaatgaag gggagcgagt ttaagccgat caaacggggc
     4981 ggtcccgaga gcgccaggaa tctgatccag caggcagagg gtaattttcg caagcagaac
     5041 gatgcttgaa gcaaacgagg ccaacccgac actttcctgt gccagaacct ggctgaagga
     5101 tctgtcggac cggaagcgca tgaccatgcg ggcaccctta atccagcggc ggggaccgca
     5161 gaccggtcca gcagtcttcc caatcgccga gggcacgaac cgggccggcg gtcagcggct
     5221 tccgtgcatc ggaaggaaca ttcgctatcg ctacttgcca tcctaccgcg cggccgacga
     5281 tccttgccct cagttacctt ccaatcggga tcaataagga gcgctcgcct ttggcatgat
     5341 tcggaaccgc tgccacaatt ttgatcgcac taatcaacaa tttttgatgg ttactcatta
     5401 agtccgaaac agcggcgaat tatgagccta attccaaaag tctgtcagag aatacgtcag
     5461 tagtggaaca aatatgacct gatatgcctg ataaatatgt ccgctgcgct ttttgtttgt
     5521 taaaacttaa tacgccgcca tctcgctgca tgacgacaaa gccggctcag gcaaccgatt
     5581 gaagcagtgc gtgtttgggc attagacacc ctttagtctg gagtgcggca tgtccggaac
     5641 ggaatccctt cgctgggaga agccgagcac gacaccggca
//
LOCUS       DASWQN010000548         4212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2032505, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000548 DASWQN010000000
VERSION     DASWQN010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032505"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /gene="boxB"
                     /locus_tag="VGC82_09175"
     CDS             complement(<1..858)
                     /gene="boxB"
                     /locus_tag="VGC82_09175"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: protein motif:HMM:TIGR03225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEY0423478.1"
                     /translation="MPDGSQIDYDGLIPNNVGLAEDLRVRKALETWHPGYIDWWKSMG
                     PEGFQNSPVYLRTAVGVGSEGWAKFGFVRMPEYRWGILLAPAIEGRTIPFGAHKGEPA
                     WQDVPGEYRAMLRRLLVIQGDTEPASVEQQRHLGATAPSLYDLRNLFQVNVEEGRHLW
                     AMVYLLQKYFGRDGREEAEDLLRRRSGHQDTPRMLGAFNEQTPDWLSFFMFTFFTDRD
                     GKMQLAALAQSGFDPLSRTCRFMLTEEAHHMFVGESGVQRIVQRTCEAMRQAGIDDPT
                     DIERVRALGV"
     gene            complement(872..2533)
                     /gene="boxC"
                     /locus_tag="VGC82_09180"
     CDS             complement(872..2533)
                     /gene="boxC"
                     /locus_tag="VGC82_09180"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HEY0423479.1"
                     /translation="MIDFQTDPTRYRHWKLSFDGPVATLTMDVDPAGGLMDGYELKLN
                     SYDLGVDIELADAVQRLRFEHPEVKTVVLRSGKEGVFCAGANIRMLGKASHGHKVNFC
                     KFTNETRLAIEDASENSGQIYLAAIAGSAAGGGYELAVTADHIMLIDDRRSSVSLPEL
                     PLLAVLPGTGGLTRLTDKRRVRRDRADIFCTAEEGVRGQRAVEWRLVDEVVPPSAWEA
                     KIAERALELAERSDRDGAKGILLAPLERTIADDAVRYPHVLVALDRSARTATVTIRGP
                     ADLPADLRGIHDSGSGFWPLAMARQLDDALLHLRLNETSIGLLIFRSVGDPAEVLAAD
                     SLLEQYQTDWLVREIRLLLKRVLKRVDMMARSLIALIDPGSCFAGTLAELVFAADRSL
                     MFAGTGTATTTTTGDNRGPATLHLSALNFSAFPMGNGLSRLANRFYGEPESLVAAAGL
                     IGETLDADAAEAHGLVTFAYDDIDWNDEVRLILEERASFSPDALTGLEANLRFVGPET
                     METRIFGRLTAWQNWIFQRPNAVGEAGSLKRYGSGVRPDFNTERV"
     gene            complement(2530..3318)
                     /locus_tag="VGC82_09185"
     CDS             complement(2530..3318)
                     /locus_tag="VGC82_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0423480.1"
                     /translation="MRAGTIQLGGGTLETGWCGPGPGEAPTLVLLHEGLGSVALWRDV
                     PERLAASTGWGVFAYSRFGYGNSDPTPLPRSMDYMHDEAQILSDVLHAAAIEQAVLIG
                     HSDGGSIAAIYAGTPKPGPRLLGLVTIAAHFFVEDLNIASIREIAATYETGDLRERLV
                     RYHRDVDAAFRGWNDAWLDPRFRDFDITGFLPNIQVPILALQGAADPYGTDEQLRVFA
                     AKARAPIATRLIPTAGHSPHLQAKDPTLAAITSFIADLPRGSTT"
     gene            complement(3318..>4212)
                     /locus_tag="VGC82_09190"
     CDS             complement(3318..>4212)
                     /locus_tag="VGC82_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEY0423481.1"
                     /translation="FHAYGLGNSLTFPMSVAAKSVFLPGRPTPDAVLDVMREQNPSLF
                     GGVPTLYAGLLANPLIGPGAGSSRLRRCISAGEALPADIGRRWRSVVGVDILDGIGST
                     EMLHIFISNRPDDIRYGASGKPVPGYEARIIDEHDRPVPHGEAGELVVRGDSAAEGYW
                     NQRAKTRRTFQGEWTYTGDTYTRDAEGYFRFQGRSDEMLKVSGIWVSPFEVEEALISH
                     PAVLEAAVIGRQDQDGLTKPKAFVILKAPDSEADALTAALQTHVKDQIGVWKYPRWIE
                     FVDSLPKTATGKIQRFRLRET"
BASE COUNT          701 a         1333 c         1452 g          726 t
ORIGIN      
        1 cacgcccagc gcgcgtaccc gctcgatgtc ggtcggatcg tcgatcccgg cctggcgcat
       61 cgcctcgcag gtccgctgca cgatgcgctg cacgcctgat tcgcccacga acatgtggtg
      121 cgcttcttcc gtcagcatga agcgacaggt tcgcgacagc ggatcgaagc cggactgtgc
      181 cagggccgca agctgcatct tgccgtcgcg gtcggtgaag aacgtgaaca tgaagaagga
      241 cagccagtcg ggcgtctgct cgttgaacgc accgagcata cgcggcgtgt cctggtggcc
      301 ggagcggcgg cgcagcaggt cttcggcttc ctcgcggcca tcgcggccga agtatttctg
      361 cagcagatag accatggccc agaggtggcg gccttcctcg acgttcacct ggaacaggtt
      421 gcgcaggtcg tacagggacg gggccgtagc gccgaggtga cgctgttgct ccacggatgc
      481 cggttcggtg tcgccctgga tgaccagcag gcggcgcagc atggcgcgat attcgccggg
      541 cacgtcctgc caggcgggtt cccctttgtg cgcgccgaac gggatggtgc ggccttcgat
      601 ggcgggagcc agcaggatgc cccagcggta ctcgggcatg cggacgaaac cgaacttggc
      661 ccagccttcg ctgccgacgc cgaccgcggt gcgcagatag accggcgagt tctgaaatcc
      721 ctcgggaccc atcgacttcc accagtcgat gtagccggga tgccaggttt ccagggcttt
      781 gcggacgcgc aaatcctcgg caaggccgac gttgttgggg atcaggccat cgtagtcgat
      841 ctggctgccg tctggcatgg gaatcatcct ttcagacgcg ttcagtgttg aaatccggcc
      901 gcacgccgga gccgtagcgc ttcagcgatc ctgcctcacc caccgcgttg ggccgctgga
      961 aaatccagtt ctgccaagcg gtcaggcggc cgaaaatgcg ggtttccatc gtttccggac
     1021 caacgaagcg cagattggct tccaggccgg tcagggcgtc gggggaaaaa gaggcgcgct
     1081 cctccaggat cagccgcacc tcgtcgttcc agtcgatgtc gtcataggcg aaggtgacca
     1141 agccgtgcgc ttcggcggcg tcggcgtcca gggtttcacc gatcagcccg gccgccgcta
     1201 ccagtgattc cggttccccg tagaaccggt tggccaggcg ggaaaggccg ttgcccatcg
     1261 ggaacgcgga aaaattcagc gccgacagat gcagcgtggc tggtccgcgg ttgtcgccgg
     1321 tggtggtggt ggtggcggta ccagtaccgg caaacatcag cgaacggtcg gcggcgaaga
     1381 cgagttcggc cagcgtgccg gcgaagcaac tgccggggtc gatcagcgcg atcaggctgc
     1441 gcgccatcat gtccacgcgc ttcagaacgc gcttcagcag cagccggatc tcgcgcacca
     1501 gccagtcggt ctggtactgc tccagcaggc tgtcggctgc gagcacttcg gcgggatcgc
     1561 cgacggagcg gaatatcagc aggccgatgg acgtttcgtt cagccgcaga tgcagcagcg
     1621 cgtcgtccag ttggcgcgcc atcgccagcg gccaaaagcc ggaaccgctg tcgtggatgc
     1681 cgcgcagatc ggcgggaagg tccgccggcc cgcgaatcgt aaccgtcgcg gtgcgggcgg
     1741 agcggtccag cgctacaagc acatgtggat aacgtaccgc gtcatcggcg attgtgcgtt
     1801 ccagcggcgc cagcaggatg cctttggcgc cgtcgcggtc ggatcgttcg gccagttcca
     1861 gcgcgcgttc ggcgatcttc gcctcccagg ccgagggcgg cacgacctca tccaccagac
     1921 gccactccac ggcgcgttgg cctcgcacgc cttcctcggc ggtgcagaag atatcggcgc
     1981 ggtcacggcg cacgcggcgc ttgtcggtca ggcgggtcag cccgccggtg ccgggcagca
     2041 cagccagcag cggcagttcc ggcaacgaca cggacgaccg ccgatcgtcg atcagcatga
     2101 tgtggtcggc ggtcaccgcg agttcgtagc cgccgccggc cgcgctgccg gcgatcgccg
     2161 ccaggtagat ctggccggag ttttcgctcg cgtcctcgat tgccaggcgc gtttcgttgg
     2221 tgaatttgca gaaattcact ttatggccgt gggaggcttt gccgagcatg cggatgttgg
     2281 ccccggcgca gaagacgcct tccttgccgg agcgcaggac gacggtcttc acctcgggat
     2341 gttcgaagcg cagccgctgc acggcgtcgg cgagctcgat gtcaacgccg aggtcgtagg
     2401 agttcagttt cagttcatac ccatccatca gtccgccggc gggatcgaca tccatcgtca
     2461 gggtggcgac cgggccgtcg aaggacagtt tccaatggcg gtagcgagtg ggatcggtct
     2521 gaaaatcgat cacgtcgttg aaccccgcgg caagtcggcg ataaagctgg tgatggcggc
     2581 cagggtgggg tccttggcct ggagatgcgg tgagtgtccg gcagtcggaa tcaggcgcgt
     2641 ggcgatcggt gcccgcgcct tggcggcgaa gacgcgtagc tgttcgtcgg tgccataagg
     2701 gtcggcggcg ccttgcagcg ccaggatcgg cacctggata ttcggcagaa acccagtgat
     2761 atcgaagtcc cggaagcgcg ggtcgagcca ggcgtcgttc cagccgcgga aggcggcgtc
     2821 gacgtcgcgg tggtaccgaa ccaggcgttc gcggaggtca ccggtttcgt atgtagcggc
     2881 gatctcgcgg atgctggcga tgttcaggtc ctcgacaaag aagtgggcgg cgatggtgac
     2941 caggccgaga aggcgtggcc cgggcttggg tgtcccggcg tagatcgccg cgatggaccc
     3001 gccgtcggaa tgcccgatca ggacggcctg ctcgattgcc gccgcgtgca gcacgtcgga
     3061 caggatctgc gcctcgtcat gcatataatc catcgaccgg ggcagggggg ttgggtcgga
     3121 gttcccgtag ccaaaccgcg agtaggcaaa aacgccccat cctgtcgatg ccgccagacg
     3181 ttccggcacg tcgcgccaca gtgccacgct gccgagacct tcatgcagca gtaccaacgt
     3241 tggcgcttcg cctggccccg ggccgcacca gccggtctcc agggtcccgc cgccaagctg
     3301 gatagtgccg gctcgcatca ggtttcccgc aggcgaaagc gctggatctt gccggtggcc
     3361 gtcttcggca ggctgtccac gaactcgatc caacgcgggt atttccagac gccgatctgg
     3421 tcctttacgt gggtctgcag cgcggcagtc aaggcatcgg cctcagaatc cggggctttc
     3481 aaaatgacga aggccttcgg cttggtcagt ccgtcctggt cttgccgccc gatcaccgcg
     3541 gcttccagca cggcggggtg ggagatcagc gcttcctcga cctcaaacgg agagacccag
     3601 atgccgctga ccttcagcat ctcgtcgctg cggccctgga agcggaaata gccctcggcg
     3661 tcgcgagtgt aggtgtcgcc ggtataggtc cactcgccct ggaacgtgcg gcgggtcttg
     3721 gcacgctggt tccagtatcc ctcggcggcg ctgtcgccgc ggactaccag ctctccggcc
     3781 tcgccatgcg gcaccgggcg gtcgtgttcg tcgatgatcc gagcctcgta gccggggaca
     3841 ggcttgccgc tggcgccgta gcggatgtcg tcgggacggt tgctgatgaa gatatgcagc
     3901 atctcggtgg agccgatgcc gtccaggatg tccacgccga cgaccgatcg ccagcgccgg
     3961 ccgatgtcgg ctggcagcgc ctccccggcg gagatgcagc gccgcagccg cgacgagccg
     4021 gcgccgggtc cgatcagggg attggccagc aggcccgcgt acagggtcgg cacgccaccg
     4081 aacaggctgg ggttttgctc gcgcatcacg tccagcaccg catccggcgt cgggcggccg
     4141 ggcagaaaca cggacttcgc ggcgaccgac atcgggaagg tcaggctgtt gccgagcccg
     4201 taggcatgga ac
//
LOCUS       DASWQN010000549         5398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2560780, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000549 DASWQN010000000
VERSION     DASWQN010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2560780"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VGC82_09195"
     CDS             complement(<1..498)
                     /locus_tag="VGC82_09195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423482.1"
                     /translation="MFASALARNLAARGIHYGWVMVALTFGYVICSSAVMSIPSVLLS
                     PISKEFGWSIGELSGPLGLRLALFGMVAPFAGALMLRYGPRSVLTGSAVLLIAGMVLA
                     MAMTARWQLWLSLGIILGIAPGMTALVLATTIATRWFTAQRGLVLGVLSAGTATGQLI
                     FLPPAA"
     gene            677..1420
                     /locus_tag="VGC82_09200"
     CDS             677..1420
                     /locus_tag="VGC82_09200"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY0423483.1"
                     /translation="MAKPAQTTSAATEWDRDAALTAARILLEIKAVNFRPDEPYTFTS
                     GWRSPVYIDCRRIIYFPQARSKICDLAVEKIQRHVGYETIETVAGGETAGIPFAAWIA
                     DRMSAPMSYVRKKPKGFGQNALIEGDVPVGKRTLLVEDLTTDGQSKIRFANALRNAGA
                     IVNHAFVVFFYGVFPGAFQTLAEMDITLHSLCTWWDVLDACKDRPYFSDAALSEVRRF
                     LENPVAWSAAHGGVSSAEEAMARKAAAEE"
     gene            1553..3172
                     /locus_tag="VGC82_09205"
     CDS             1553..3172
                     /locus_tag="VGC82_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0423484.1"
                     /translation="MPRMTGGEAIVDSLLRHGIDTVFGLPGVQTYGLFDAFARSSNRL
                     RLINARHEQTTAYMALGYACATGRPAAYAVVPGPGVLNTTAALATAWGVNAPVLCLTG
                     QVPSAMIGRRRGQLHELPDQLATLRTLLKWAERIDHPSQASHLVARAFQEMLSGRRGP
                     VALEMPWDQFTATADVTPLEPLPPHPNPIPDPEKIAALAKLLDGAKAPMLWVGGGALH
                     AAAEIQALAERIGAPVVSFRNGRGIVDDRHPLGLTIPAGYRLWPKTDLLVAFGTRLEV
                     PTGRWGAMPAGLKIARIDIDPAEMPRVKVDIGIVADTADAAQALTDMVTPRNDSEQAA
                     VVARAKADTLADIQRVQPQMSFLKAIREVLPENGILCDEMTQVGYVSWFGFPIHTPRT
                     LITSGFSGTLGAGFPTALGVKVAMPDRPVVSLNGDGGFLFGGAELATAMQHRINLVTI
                     VFNNSAYGNVLRDQRRLFEGRESGSALRNPDFLMFARSFGVPAWRVSDAGGLRDALSH
                     ALLEKSPTFIEVMTDITQETGPWEFIAPGRG"
     gene            complement(3418..>5398)
                     /locus_tag="VGC82_09210"
     CDS             complement(3418..>5398)
                     /locus_tag="VGC82_09210"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0423485.1"
                     /translation="NPVRQEALIALGGLLLIRGEPEAARDLLLRSCGIASDNAEAWNT
                     LGLALSATGAPGLALSAFLKAQALQPDRLDYVLNGVDVTLDANEGEAELPRLSVACEQ
                     NPLNPVMHIGRGMLLERLGHRDDAIDAVEAATELAPDALVPLRLLGGLLARSGRVHQA
                     EQVLRRISSLDPDNPGVQNDHAAVLMRLHRHAEARAILQQILDRHGPHPSVLCNLANA
                     TACVGKQDEAVATARRAIELDPAATLPRRALCSTLPYRDGTSGAELLSEMRHYSALLP
                     RVAQPRFTNRPDPERKLVVGLLSGTLRSHPVGWLTVAGFETLDPDQFSLVCMTQNAAP
                     KDPIARRFRAAAGDWVEVDSLTDADLTALARAKGIDILIDLGGYGDAARMPACANRLA
                     PVQIKWVGMQTHSTGLAEMDWFMTDRWETPEGFEPLYSEKLLRLPDGYVCYSPPTHAP
                     DVVELPALKNGFVTFGCFNNLAKITPRVVETWTTILRLVPDSKLILKTHQLSDQGTAD
                     GFLADFSALGIGGDRLELRGSSGHRAFMSEYGDVDIVLDPFPYSGGLTTCEALWMGVP
                     TITLPGEIFASRHSASHMSNAGLETWVSSSVETYVKMAVDRAGSVEDLAILRAGLRER
                     VRNSPLCDAPRFGRNLGAALRQVWRAWCADEDREP"
BASE COUNT         1008 a         1749 c         1673 g          968 t
ORIGIN      
        1 cgccgccggg ggcagaaaga tcaactgacc tgtggcggtg ccggcactca ggacacccag
       61 caccagacca cgttgcgcgg tgaaccatcg cgtggcgatg gtcgtcgcca gaaccagcgc
      121 ggtcattccc ggcgcgatcc ccaatatgat tcccaggctt agccacaact gccagcgcgc
      181 ggtcatcgcc atggcgagca ccatgccggc gatcagcaac accgccgatc cagtcaacac
      241 cgaacggggg ccgtagcgca gcatcaatgc gcccgcaaag ggagcgacca tgccgaacag
      301 cgccaggcgc aaacccagtg gcccggacag ttcaccgatg ctccagccaa actccttgga
      361 aatcggcgac agcaaaacgc tggggatact catgaccgcg gagctgcaga tgacgtagcc
      421 aaaggtcagc gccaccatga cccagccata gtggatcccc cgggcggcga gatttcgtgc
      481 gagcgcgctg gcaaacattc cgacctcgat atgccgatga gcgagcgtcc gattgacgcg
      541 aagcgtgacg catagcccca ataactatca tcatgcaagt ttgtatctca tggcgatccg
      601 tggcaccggg gctgtcaggg atcggcgaaa tcggctatcg gggtgcggat caaccagcac
      661 tacgggatcc gccaagatgg caaaacctgc ccagaccacc tccgctgcca ccgagtggga
      721 ccgtgatgcc gccctgaccg ccgcgcggat cctgctggaa atcaaggcgg tcaatttccg
      781 ccccgatgag ccgtacacgt ttacctccgg ctggagatcc ccggtctata tcgattgccg
      841 ccggatcatc tatttcccac aggctcgctc gaagatctgc gatctggcgg tggagaagat
      901 ccaacgccac gtaggttatg aaacgatcga gacggtggcc ggcggcgaga ccgcgggcat
      961 tcccttcgcc gcctggatcg ccgatcggat gtcggctccg atgtcctacg tgcggaagaa
     1021 acccaaaggc ttcggccaga acgcgctgat cgaaggcgac gtgccggtgg gcaagcgtac
     1081 cttgctggtg gaggatctca ccaccgacgg tcagtcgaaa atccgcttcg ccaacgcgtt
     1141 gcgcaatgcc ggcgcgatcg tgaaccacgc cttcgtggtg ttcttctacg gggtctttcc
     1201 cggtgcgttc caaaccctgg cggagatgga catcacgctg cacagcctgt gtacctggtg
     1261 ggacgtgctg gacgcctgca aggaccggcc atacttttcg gatgcggctt tgtccgaagt
     1321 gcggcgtttc ctggaaaacc cggtcgcgtg gtcggccgcg cacggtggtg tatcctcagc
     1381 cgaggaagca atggcccgaa aagccgccgc cgaggagtag cgtgacggct cgccccggat
     1441 ttcggcgcga cggtcgcttg agagatttca ggactgtccg ctgatctcgc cagccttccc
     1501 cgcgtccacg ggacatgcca acgtcggatc acagaacatc aggggttgat ccatgccacg
     1561 catgaccggc ggagaagcga tcgtcgatag ccttttgcgg cacggcatcg atacggtgtt
     1621 cggcctgccc ggagtgcaga cctacgggct gttcgacgcg ttcgcccgat cctccaaccg
     1681 ccttcgcctg atcaacgcgc gtcacgagca aaccaccgcg tacatggcgc tcggatacgc
     1741 ttgcgcgacg ggacgacctg ctgcctatgc ggtcgtgccg ggacctgggg tgctcaacac
     1801 gacggcggcg ctggcgacgg cctggggggt caacgcgccc gtgctgtgcc tgaccggcca
     1861 ggtgcctagc gcgatgatcg gtcgccgccg cggccagctt catgaactgc cggatcagct
     1921 cgcgaccctg cgcacgctgc tgaaatgggc agagcgcatc gatcatccgt ctcaggcgtc
     1981 gcatctggtc gcgcgcgcct tccaggagat gttgtcgggc cgccgcggcc cggtggcgct
     2041 ggaaatgccg tgggaccagt tcaccgcgac cgccgacgtc accccgcttg agccgctgcc
     2101 gccgcacccc aatccgatcc cggacccgga gaagatcgcg gccctggcga agctgctgga
     2161 tggtgcgaag gcgcccatgc tgtgggtggg cggcggcgca ttgcatgccg cggcggaaat
     2221 ccaggcgctg gcggagcgaa tcggcgcgcc cgtcgtctcc ttccgcaacg gccgtggcat
     2281 cgtcgatgac cgccatccgc tcggtcttac catcccggct ggctaccggc tttggccgaa
     2341 gaccgatctg ctcgtcgcgt tcggcacgcg gctggaggtc ccgactggtc gttggggtgc
     2401 gatgcctgcc ggattgaaga tcgcccgtat cgacatcgat cccgccgaga tgccacgcgt
     2461 aaaggtcgat atcggcattg tcgcggatac ggccgatgcc gcccaggcat tgaccgacat
     2521 ggtcacgcct cgcaatgaca gcgaacaggc tgccgtggtg gcgcgtgcaa aggccgatac
     2581 gctggcggac atccagcgcg tgcagccgca aatgtcgttt ctgaaggcga tccgggaggt
     2641 gctgccggaa aacggtatcc tctgcgacga gatgactcag gtcggctatg tttcctggtt
     2701 cggcttcccg atccacacgc cgcgcacgct gatcacctcc ggcttctccg gaacgcttgg
     2761 cgccggcttc ccaacggcgc ttggggtgaa ggtggcgatg ccggaccgcc ccgtcgtgtc
     2821 gttgaacggc gacggcggct ttctgttcgg tggagcggaa ctggcgacgg caatgcagca
     2881 tcgcatcaat cttgttacca tcgtgttcaa caactcggcc tacgggaacg tgctgcgtga
     2941 ccagcggcgg ctgttcgaag gccgcgagtc aggttcggct ttgcgcaatc ccgacttcct
     3001 gatgtttgcg cgcagctttg gcgttcccgc ctggcgcgtt tcggatgcgg gcggtttgcg
     3061 cgacgcgctg tcgcacgcgc tgttggaaaa atccccgacg ttcatcgagg tcatgaccga
     3121 catcacgcaa gagaccgggc cgtgggagtt catcgcgccg ggccgcggct agaccatttt
     3181 ccgttcgcct gggctcactg aaaacggtct agctcattgt cttagcgagt atcttcaccc
     3241 gttcagataa tctatctgaa cgggatctgc tctagagaat gatcccaacg actgcctcac
     3301 cgtcatgcaa gccctgatgg gggcaaaacc tccagccgat attgcgatgt gaacccatgg
     3361 ccgccacgcg acgaccgggc ctttttgtct ggtcgccagc gtgagcatgg ggtggaatta
     3421 cggctctcgg tcttcatcag cgcaccatgc tcgccagacc tgccgcaggg ctgcgcccag
     3481 gtttcgcccg aaccgaggcg cgtcgcaaag tgggctgttg cgaacccgct cccgcagtcc
     3541 tgcccgcagg atcgccagat cttcgacgct cccggctcga tcgacggcca tcttgacgta
     3601 ggtctcgacg gaacttgata cccaggtttc cagtccagcg ttgctcatgt ggctggcaga
     3661 atgccgcgac gcgaaaatct cgccgggcag cgtgattgtc ggcacaccca tccagagcgc
     3721 ctcgcacgtc gtcaggccgc ccgagtaagg aaacggatcc agcacgatat cgacatcgcc
     3781 atactcgctc atgaacgcgc ggtggccgga cgatccacgt aactccaacc gatcgccgcc
     3841 gatgccgagt gctgaaaagt ctgcgagaaa gccatcggcg gtgccctggt cggacaattg
     3901 gtgcgttttg aggatcagct tgctgtcggg caccaaacgc aagatcgtcg tccatgtctc
     3961 gaccacccga ggtgtgatct tcgccaaatt gttgaaacag ccaaacgtga cgaagccgtt
     4021 cttcaaagcc ggcagttcaa ccacgtcggg cgcatgcgtt ggcggactgt agcaaacgta
     4081 tccgtctggc aggcgaagca gcttttcgct gtaaagcggc tcgaaccctt ccggagtttc
     4141 ccagcgatcg gtcatgaacc aatccatttc cgccaaaccg gtactgtggg tctgcatccc
     4201 gacccacttg atctgcaccg gcgctagccg gttggcgcaa gccggcatcc gtgccgcatc
     4261 gccgtagcct ccgaggtcga tcaaaatgtc gatgcccttg gcccgcgcga gggcggtcag
     4321 atccgcatcg gtcaaactat cgacttccac ccagtcaccc gcggcggccc gaaagcggcg
     4381 ggcaataggg tccttcggcg ctgcgttctg ggtcatgcaa accaagctga actggtcggg
     4441 gtcgagggtc tcgaacccgg caaccgtgag ccagcccacc gggtgcgacc gcagggttcc
     4501 cgacagcaaa cccacgacaa gcttccgttc cggatcgggc ctgttggtga accgcggctg
     4561 cgcaacacgc ggcaacagcg cggagtagtg acgcatttcg gacaacaatt ccgcgcccga
     4621 tgtaccgtcg cgatatggaa gcgtgctgca cagggcacgc cggggcagcg tcgcggcggg
     4681 atcaagctcg attgcccgcc gagcggtcgc aactgcttca tcctgtttac cgacgcaggc
     4741 cgtagcgttg gccaggttgc acaggacgga cggatgcgga ccgtgccgat caagtatctg
     4801 ctgcagaatt gcccgtgcct cggcgtgccg gtgcaatcgc atgagcacgg cagcgtgatc
     4861 gttctgcacc cccggattat cgggatcgag gctgcttatc cgccgcagca cctgttccgc
     4921 ctgatgaacc cggccggagc gcgccagcag cccacccaac aaacgcaacg gaaccagcgc
     4981 gtccggagcc agttcggtcg cggcttcaac ggcgtcgatt gcatcgtcgc ggtgtcccag
     5041 ccgctccagc aacatcccgc gtcctatgtg cataacggga ttgagtggat tttgctcaca
     5101 ggcaacgctc aggcggggca gttcggcctc gccctcattg gcatcaagcg tgacgtcaac
     5161 cccattcagg acatagtcca gacggtccgg ctgtagcgcc tgggccttga ggaacgccga
     5221 tagtgccagg cccggtgctc cggtcgcgct taaagccagg ccgagggtgt tccaagcctc
     5281 cgcgttgtcc gatgcgatgc cgcagcttcg aagcagcagg tcacgcgccg cctcgggctc
     5341 gccacgaatc agcaggagcc cgcccaaggc gatcaatgcc tcttgccgga ccgggttc
//
LOCUS       DASWQN010000550         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1373352, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000550 DASWQN010000000
VERSION     DASWQN010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373352"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /locus_tag="VGC82_09215"
     CDS             <1..1515
                     /locus_tag="VGC82_09215"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY0423486.1"
                     /translation="ATGGNNSSFAYSGTMAGADANVAAATAAYTTLSNMFTSPVWSTP
                     IGVLNPALAGNTSNTTLATNIIIPQLKAFLASELTSLGLSSPATVCASANSALCNGYN
                     LGIAAATAVNNSSTFSPTASGAVAAITAGLTANAPAGSGTTPGVYVPPAARPEMFPTW
                     GGTALTGITSTQMNAAKATVSGPPAITSAAYAAALLQTECQGGSAALSTRPVSIQSAC
                     ATAGFTQETTAQTKAALFWNDPGTTIQPPGHWLQIANAAMLSQGSSLLQSAQLTALMG
                     EAMNDAGILAWDEKYTNNLWRPVTAIRDCSNWSAGNFTTCDASWSSLIATPPHPDYIA
                     GHPAFSGAAATVLERFFGTDNIAFTSTSNYYCNGAASQFDPATNLLASCTVGSGPSAI
                     VYAVSNPSDCAVIVDGINSNGSPLICPISETFNSFSDASQGPNGSEFSRVVGGIHTPF
                     SVEDALAAGNFIGQALAQNAGLPELVPEPSSLGLCGIGLLSLTRLRRRRHAVTA"
     gene            1536..>2065
                     /locus_tag="VGC82_09220"
     CDS             1536..>2065
                     /locus_tag="VGC82_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423487.1"
                     /translation="MKRFQAAILGVALMLGAGAADPPVAPLEVRAEALAASLAAACPL
                     GHYDDEAAFEACGRTLRTIGLPFGPAVAWGGDQPDRQIKKKGLTHINSQVFRAMYLPL
                     FSFTGRWSLDEDKRSHTPIVRLEAYFRNALPPGDFPYPFWHSADKWNAYEVANELRFY
                     LDLKGIAFVVTRGAAG"
BASE COUNT          370 a          729 c          601 g          365 t
ORIGIN      
        1 gccaccgggg gcaacaattc ctcattcgcc tactccggga cgatggccgg tgccgatgcc
       61 aatgtggcgg ctgccaccgc ggcttacaca acgctttcca acatgttcac cagtcccgtt
      121 tggtcgacgc caatcggcgt cctcaacccc gcgctggccg gcaatacctc caacaccacc
      181 ctggcgacca acatcatcat cccacaactc aaagcatttt tggccagcga actgacctcc
      241 ctggggttat ccagccccgc cacggtatgt gcctccgcca attccgccct ctgcaacggc
      301 tataatcttg gcatcgcggc ggcgaccgcc gtgaacaact catcgacctt cagccccacg
      361 gcttccggcg cggtcgcagc gatcaccgcc gggctgaccg ccaatgcacc agccgggtcc
      421 ggtaccacac ccggcgtcta tgtgccgccc gccgccaggc cggaaatgtt cccaacctgg
      481 ggcggcaccg cgctgaccgg cataaccagc acgcagatga acgctgccaa ggccacggtc
      541 tcggggccgc cggccatcac ctccgcggcg tacgccgcag ccttgctcca gaccgagtgc
      601 cagggcggga gtgcggcgct gagcacgcgg ccggtcagta tccaatcggc ctgcgccacg
      661 gcaggcttca cccaggaaac caccgcccag acgaaggccg cactgttctg gaacgatccc
      721 ggcacgacca ttcagccgcc cggtcattgg ctgcagatcg ccaatgccgc gatgctcagc
      781 cagggcagca gcctgctgca aagcgcgcag cttaccgcct tgatggggga ggccatgaac
      841 gacgccggca tcctcgcctg ggacgagaaa tataccaaca atttgtggcg tcccgtcacc
      901 gcgatccgcg attgcagcaa ctggagcgcg ggcaatttca ccacctgcga tgcctcctgg
      961 tcgtcgttga tcgcgacacc gccccatcct gactatatag ccggccaccc cgcgttcagc
     1021 ggcgcggcgg ccacggtgct ggagcgtttc ttcggcaccg acaacatcgc cttcacctcg
     1081 acatccaact attactgcaa tggcgccgcg tcgcagttcg atcccgccac gaacctgctg
     1141 gcctcctgca ccgtgggcag cggtcccagc gcgatcgttt acgcggtgtc caatccgtcc
     1201 gactgcgccg tgatcgtcga tggcatcaac agcaacggca gcccgctgat ctgtccgatc
     1261 agcgaaacgt tcaacagttt ttcggacgct tcgcagggac cgaacggatc ggaattcagc
     1321 cgcgtcgtcg gtggcatcca cacgcctttc tcggtggagg acgccttggc cgcagggaat
     1381 ttcatcggcc aggccctggc acaaaacgcg ggattgccgg aactcgtgcc ggaaccgtcc
     1441 agcctgggcc tctgcggcat cgggctgctg tcgcttaccc gcttgcgccg ccggcggcac
     1501 gcggtcaccg cctgatcctg ctggacggta tttcgatgaa acgttttcag gctgccattc
     1561 ttggtgtggc tttgatgctg ggcgccgggg ccgccgatcc tccggtggct ccgcttgaag
     1621 tccgcgccga ggccttggcc gcgagcctcg ccgccgcctg tccgttgggg cattacgatg
     1681 acgaggcggc gttcgaggcc tgcggccgaa cattgcggac gatcggactg ccgttcgggc
     1741 cggccgttgc ctggggcggc gaccagcccg acaggcagat caagaagaaa ggtctgaccc
     1801 atatcaacag ccaggtcttt cgggccatgt atttgccgct gttcagcttc accggacgct
     1861 ggtcgctgga tgaagacaaa cggtcccaca ccccgatcgt ccggctggaa gcctatttcc
     1921 gcaacgcgtt gccgcccggc gactttccct atccgttctg gcacagcgca gacaaatgga
     1981 acgcttacga ggtagccaac gagctgcgat tctacctcga tttaaaaggg atagccttcg
     2041 tcgtgacccg cggcgccgcc ggcag
//
LOCUS       DASWQN010000551         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1901353, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000551 DASWQN010000000
VERSION     DASWQN010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901353"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VGC82_09225"
     CDS             <1..988
                     /locus_tag="VGC82_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0423488.1"
                     /translation="FLAHILAHPRCGTHLCHAMLLPRPEAAAALAALDARGYVDLGPV
                     RVERHGRAAHLLSSNPRYLNAEDQGTIDAMEIGVDVATLDPGTDIAVLRGQPVQNAKY
                     AGRRIFGSGINLTHLYRGTIPFLWYLQRELGFVHKYLRGVALADVLPDDVNGSGTEKP
                     WIAAVDGFAIGGHCQILLTMDYVLAAADAFMTLPARKEGIIPGAANLRLPRFTGDRLA
                     RQLIQAERRLPCDSPEGRLICDEIVPPEDMDAAIDRTVAMLTDAGAVGAIGNRRAFRV
                     AVEPLDLFRRYCAVYAREQASCHFSPALIRNLEQNWGAGAKSGSSKAGGLSG"
     gene            1206..>2054
                     /locus_tag="VGC82_09230"
     CDS             1206..>2054
                     /locus_tag="VGC82_09230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0423489.1"
                     /translation="MATSDRWQKPSGFLPIVGPVLDSLITPITAEGAAIVALDDHGGM
                     PVQLYATRPSPPAMLDAVTLLSAGSMAPSHGMCPGEYSILAGPWQVGSGPKLVLVFWR
                     EKAARPWKKQDHALVLTAAAALTALLMHGVQPRTDADRRGIVDSITGLPKARKFITEL
                     PRYFARLDRENLPGSLILVNVVGYDRLIAELGRKTGDTVLRKAAELLVRVTRPADFIA
                     RLRDDEFAMWLSGADHFTAAERAEQLRIDGPGVLSMAVKNGVLLASLSVGIATRHSGS
                     KETPGSL"
BASE COUNT          347 a          633 c          684 g          390 t
ORIGIN      
        1 gttcctggcg catatcctgg cgcatccgcg ctgtggcacg catctgtgcc acgccatgct
       61 gctgcctcgt ccggaagcgg cggcggcgct ggcggcgctg gatgcccgcg gctacgttga
      121 tctcgggccg gttcgggtgg agcggcatgg acgggcggca caccttctga gcagcaatcc
      181 ccgctatctg aacgccgaag atcagggcac catcgacgcg atggagatcg gcgttgacgt
      241 cgcgacgctg gaccccggga cggacatcgc ggtcctgcgg gggcagccgg tgcagaacgc
      301 caaatatgct gggcggcgca tcttcggcag cggcatcaac ctgacgcatt tgtatcgcgg
      361 caccattccg tttctttggt atctgcaacg ggaacttggc ttcgtgcaca aatacctgcg
      421 cggcgtggcg ttggcagatg ttttacccga cgacgtcaat ggctccggga cggaaaagcc
      481 atggatcgcg gccgtggatg gttttgcgat cggcggccac tgccagattt tgctgacgat
      541 ggactatgtc ctggcggctg ccgatgcctt catgacgctg ccggcccgca aggagggcat
      601 catccccggc gctgccaatc tgcggcttcc gcggttcacc ggcgatcgcc tggcaaggca
      661 gcttatccag gccgaacggc ggttgccgtg cgacagtccg gaagggcgcc tgatctgtga
      721 cgaaatcgtg ccgccagagg acatggacgc ggcaatcgac cgcacggtgg cgatgctgac
      781 cgacgccggg gcggtcggtg cgatcggcaa ccggcgggcg ttccgcgtcg ccgtcgagcc
      841 gctggacctg ttccgtcgtt attgcgctgt ttatgcacga gaacaggcgt cgtgccattt
      901 cagcccggcg ctgatccgga acctggagca gaactggggc gcgggcgcca agtcgggatc
      961 gtcgaaagcc ggagggcttt caggctgacc ggaaacggtg cgagccggtt tgccgcctga
     1021 cggcgatgtg ccatggccgt gcctggcgct tccatcagtc acaacagtat cctgttcccg
     1081 ggcgccgtga aaccgctcca gacttcccgt tccgtaacga ggttgcaaac gctcattccc
     1141 ctcgacattt cggtcgttca catgttagtg agtgaacgtt gcaaattcgt gggatcccta
     1201 tggcaatggc gacttccgac cgctggcaga aaccgtccgg cttcctgccg atcgtggggc
     1261 cggtgctgga ttcgcttatt acgccgatca cggcggaagg ggccgcgatc gtcgcactgg
     1321 atgaccacgg cggcatgccg gtccagctat acgcgacgcg gccaagccct ccggcgatgc
     1381 tcgatgccgt gacattgctg tccgccggat cgatggcgcc gtcgcatgga atgtgccccg
     1441 gcgagtattc cattctggct ggtccatggc aggtcggttc gggaccaaag ctggttctgg
     1501 tcttctggcg ggagaaagcg gccaggccgt ggaaaaagca ggaccacgcg ctggtcttga
     1561 cggccgccgc cgccttgacc gcattgctca tgcatggggt ccagccgcgg acggatgccg
     1621 accggcgcgg catcgtcgac agcatcaccg ggcttccgaa ggcgcgcaag ttcatcacgg
     1681 agttgccgcg gtatttcgcc cggctggatc gcgagaatct gcctggcagc ctgatcttgg
     1741 tcaacgtcgt cggatatgac cggctcattg cggaattggg acgcaagacc ggagacacgg
     1801 tgctgcggaa agccgctgaa cttcttgtcc gcgtcacccg gccggcggat tttatcgctc
     1861 gcctccgaga cgacgagttc gccatgtggc ttagcggggc cgaccatttc accgcggccg
     1921 aacgcgccga acagttgcgg atcgatggcc cgggcgtcct gtcgatggcc gtaaaaaacg
     1981 gcgttctgct tgccagtctt tcggtcggga tcgcaacgcg acactccggc agcaaggaaa
     2041 cgccaggcag cttg
//
LOCUS       DASWQN010000552         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1241270, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000552 DASWQN010000000
VERSION     DASWQN010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241270"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VGC82_09235"
     CDS             <1..942
                     /locus_tag="VGC82_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEY0423490.1"
                     /translation="RMLVGTPSGEGFRVLDSFSRIVRLGEGLHSTGALNEAAMDRAIC
                     ALQTCAAKLARRSVRQVRAIATAACRRATNGPAFLCRVKQETGFDFGIISSREEAELA
                     LESCTPLLPCDGRRALLFDIGGGSTELAWVRLTNGRPELIGYESLPIGVVTLAERWGD
                     AGFTPDGFEAMVSDIVERLRAFEKVHCIAREIRDGGVRLLGTSGTVTTLAGVALNLER
                     YRRLAVDGVELCSKEAADALLHLRSLGREGLMLHPCVGPERVEFVLPGCAVFAAIARI
                     WPAPHVIVADRGLREGVLLRLMRADRLRQGHRREFCR"
     gene            953..1639
                     /locus_tag="VGC82_09240"
     CDS             953..1639
                     /locus_tag="VGC82_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEY0423491.1"
                     /translation="MAKTPVMPPSRGLSIHVKSTKSRTPASARWLLRQLNDPYVTAAR
                     KQGWRSRAAFKLLELDDKFHLIHKGIRVLDLGAAPGGWTQVAVKRGAGSVLALDLLPI
                     DPILGATILQGDFSDPGMPQRLITELGGDADLVLSDMAPNTTGHSATDHIRIVALAEM
                     ALDLAIQILSPGGGFVAKVFQGGSEKQILDVLKRNFGSVRHAKPPASRKESSELYVVA
                     TGFRGRMGGG"
     gene            complement(1880..2137)
                     /locus_tag="VGC82_09245"
     CDS             complement(1880..2137)
                     /locus_tag="VGC82_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY0423492.1"
                     /translation="MVTLPVALLRLFPGCEPEIAVSATHIDQMIDVLDSRWPGMRDRL
                     CDTSPKIRRHINIFVDGERAVLETPLAAGAEVFIMTAISGG"
     gene            complement(2159..>2583)
                     /locus_tag="VGC82_09250"
     CDS             complement(2159..>2583)
                     /locus_tag="VGC82_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEY0423493.1"
                     /translation="HCGMYRSDDGGRNWESIEAGLPSSFGFPVVAHPRDPSTLFLVPL
                     NGDIAGRYVPDGKAAVWRTRDAGGSWQPMRTGLPQENAFFGVLRQAMAADWLEPAGIY
                     AGTNTGNLFASADEGETWTSIAQHLPPILSVETLVLDA"
BASE COUNT          410 a          797 c          877 g          499 t
ORIGIN      
        1 cggatgctgg tgggcacgcc ctcgggtgag ggatttcgtg tgctcgacag tttctcccgg
       61 atcgttcgtc tgggtgaggg gctgcacagc acgggggctc tgaacgaagc tgcgatggac
      121 cgcgcgatct gcgccctgca gacctgcgcc gccaaactgg ctcggcgttc ggtgcgccag
      181 gttcgcgcca ttgccaccgc ggcctgccgc cgcgccacga acggccccgc ctttctgtgc
      241 cgcgtgaagc aggaaaccgg cttcgatttc gggatcatct ccagccgcga ggaagccgaa
      301 ctggcgctgg agtcatgtac gccgctgctg ccatgcgacg gacgccgggc gctgttgttc
      361 gatatcggcg gcggctcgac cgaactggcc tgggttcgcc tgaccaacgg ccgcccggag
      421 ttgatcggct acgagtcgct tccgatcggc gtcgtaaccc tggccgaacg ctggggcgat
      481 gccggtttca ccccggacgg cttcgaagca atggtgagcg acatcgtcga gcgcctgcgc
      541 gcattcgaga aggttcattg tatcgcgcgc gaaatccgcg atggcggggt gcgtctgctg
      601 ggcaccagtg gcacggtcac caccttggcg ggcgtcgcct tgaaccttga acgctatcgc
      661 cgattggcgg tggatggcgt cgaactgtgc agcaaggagg cggccgacgc attgttgcat
      721 ttgcgctccc tcggacgaga ggggctgatg ctgcatcctt gcgtcgggcc tgagcgcgtc
      781 gagttcgtgc tgccgggctg tgccgtgttc gctgccatcg ctcggatttg gccggcgccc
      841 catgtgatcg tggcggatcg cggcctgcgt gagggcgtgc tgctgcggct gatgcgtgct
      901 gaccgcctgc gtcaggggca tcggcgcgag ttctgccgct gatgcgtgtc tgatggccaa
      961 gacgccggtc atgccgccct cgcgggggtt gtccattcac gtcaagagca cgaagagccg
     1021 cacgcctgcg tccgcgcgct ggctgcttcg gcaactgaac gatccgtatg tcacggcagc
     1081 cagaaagcaa ggctggcggt cgcgggcggc gttcaaattg ctggaactgg atgacaaatt
     1141 ccatctgatc cataaaggga ttcgggtgct ggatcttggc gcggcaccgg gcggctggac
     1201 ccaggtcgcg gtaaaacgag gcgcggggtc ggtattggca ctcgacctgt tgccgatcga
     1261 tccgatcctc ggtgcgacca ttctgcaggg cgacttcagc gaccccggaa tgccgcagcg
     1321 gctgatcacg gaacttggcg gcgacgcgga tctggtgttg tcggacatgg cgccgaatac
     1381 caccgggcat tcagcgaccg atcacatccg catcgtggcc ctggccgaaa tggcgctgga
     1441 tcttgccatc cagatattgt cgccgggtgg cgggttcgtg gcgaaggtct ttcagggcgg
     1501 ttcggagaag cagatcctgg atgtgctcaa gcggaatttt ggcagcgtcc ggcatgcgaa
     1561 gccgcctgct agccggaagg agtccagcga gctttatgtc gtcgccacgg ggtttcgggg
     1621 gcggatgggc ggtggctgat ctggctgtgg cgcgaagaaa gccgttcatc atggattgca
     1681 ccgaggcagg tttttgccgt ggggttgaac acggcggcgt cgctgtttca gagctttttg
     1741 cgcttcgcgc ggcaggttct tgcttctgtg tcggcctccg gttcctggac gggcttcgac
     1801 gaccgtccgt gctgatccgt gactgaaacc gcgcaatccg tgatgaaacg ctttgcctga
     1861 tgccgcttcg tccgacggac tatcccccac tgatcgccgt catgatgaac acctccgcac
     1921 cagcggcaag cggcgtttcc aacaccgcgc gttcgccgtc cacgaagatg ttgatgtggc
     1981 gccggatctt cgggctcgta tcgcaaagcc gatcacgcat acccggccag cgcgaatcca
     2041 gcacgtcgat catctgatcg atatgcgtgg ccgagaccgc gatctccggt tcgcagccgg
     2101 ggaacaggcg caggagcgca accggcagcg tcaccacgac gcgttcgtcc tgcatccgct
     2161 acgcgtcgag aaccagcgtc tccaccgata gaatcggcgg cagatgctgg gcgatgctgg
     2221 tccaggtttc gccttcatcg gcgctggcga acagattgcc ggtattggtg ccggcataga
     2281 tgcccgcggg ttccagccaa tccgccgcca tggcctggcg caggacgccg aagaacgcgt
     2341 tctcctgcgg caggccggtg cgcatcggtt gccagcttcc gcctgcatcg cgtgtgcgcc
     2401 agactgcggc ctttccgtct gggacatagc gcccggcgat gtcgccgttc agcggcacca
     2461 ggaacagcgt cgacgggtcg cgcggatggg caaccacggg aaagccgaag ctcgacggca
     2521 ggcccgcctc aatgctctcc cagttccggc caccgtcgtc gctgcggtac atgccgcaat
     2581 ggt
//
LOCUS       DASWQN010000553         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_317792, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000553 DASWQN010000000
VERSION     DASWQN010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317792"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VGC82_09255"
     CDS             <1..545
                     /locus_tag="VGC82_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0423494.1"
                     /translation="TQADIGYRANNALIAHGDTIVIPADATEQVQYEGELVVVIGRKC
                     KNLTRDNALSAVLGYTIGNDVSERSWQKADRTMWRSKNTDTFKPMGPWIETDVKLEDL
                     VTTIRLNGEQLIAFPTNHMIFGVVDYLVSMTSCLTLYPGDVVWMGTEGATANMKHGDR
                     IEVEISGIGTLSNPVVREGM"
     gene            586..855
                     /locus_tag="VGC82_09260"
     CDS             586..855
                     /locus_tag="VGC82_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423495.1"
                     /translation="MPATISTNPAKQSLHPTRLFCAAARAAKEHGKSMGIGGARHDVD
                     FQSWLLKLGVRYLISGLDTLYILSGARADVAQIREVAAGASAGTP"
     gene            complement(815..1894)
                     /locus_tag="VGC82_09265"
     CDS             complement(815..1894)
                     /locus_tag="VGC82_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0423496.1"
                     /translation="MGRKTVLVAGATGLVGYAALKHFAAAPDTDVVAVSRRLPPGIEV
                     RHLALDLTNPVACRHAAETLRDVTHVVYAALFELPGLVEGWRNDQQISTNDRMLRNLL
                     GPLLQVAPGLRHVTLLQGTKAYGVHVRPLAVPAREGRSEMREQPNFYWTQEDYLRAAQ
                     AGKDWTFTILRPVLIVGEAIGGAMNLIPALGAHAAVMRERGPILPYPGGAERVSQAVD
                     ADLLARAIAWAGDTPGARNETFNVTNGDVFTWPNIWPTIAETLGMRSGPDEPFLLQSL
                     GRADWDRIRQRHGLLSPTLTDFVGLSLEYADYQMRYGHTEPGPPAIVSTIKIAQAGFH
                     EVMDTEVMFQRWFKVFQQKRLLPLP"
BASE COUNT          464 a          679 c          749 g          396 t
ORIGIN      
        1 cgacgcaggc ggatatcggg tatagggcga acaacgccct gatcgcccat ggtgacacga
       61 tcgtcattcc ggcggatgcg accgaacagg ttcagtatga gggtgaactc gttgttgtca
      121 ttggccggaa atgcaagaac ctgacccgcg ataacgcctt gtccgcggtg ctcggttata
      181 ccatcggcaa cgacgtgagc gagcgcagtt ggcaaaaggc ggatcgcacg atgtggcggt
      241 cgaagaacac tgacacgttc aagccgatgg gcccatggat cgaaaccgat gtaaagcttg
      301 aagacctggt tacgacaatc cgcctcaacg gcgagcaact gattgctttt ccgacaaacc
      361 acatgatctt tggcgtcgtg gactacctgg tttcgatgac gagctgcctg acgctgtatc
      421 cgggcgacgt cgtctggatg ggtaccgagg gtgccaccgc caacatgaag cacggcgacc
      481 ggatcgaggt cgagatcagc gggatcggga cgttgagcaa tccggtggtg cgggagggga
      541 tgtagatacg gatcggctgt tggccgcgag cccgggggga acaacgtgcc agcaacaata
      601 agcaccaacc ccgccaagca aagcttgcat ccgacgaggt tgttctgtgc cgccgcccga
      661 gcggcgaagg agcatggcaa gtcgatgggc atcggcggcg cgcgccacga tgtggacttt
      721 cagtcatggc tgctgaagct gggcgtgcgc tacctgatca gcgggttgga cacgttgtat
      781 atcctcagcg gcgcacgggc cgacgtggcg cagatcaggg aagtggcagc aggcgcttct
      841 gctggaacac cttgaaccac cgctggaaca ttacttcggt gtccatgacc tcgtgaaagc
      901 cggcctgcgc gatcttgatg gtcgacacga tcgccggcgg gccgggttcg gtatggccat
      961 aacgcatctg gtagtccgca tactccagcg acagcccgac gaaatccgtc agcgtagggg
     1021 acaacagccc atgacgctgg cggatgcggt cccagtcggc gcgccccagc gactgcaaca
     1081 ggaatggctc gtccggacct gaacgcatcc caagcgtctc ggcgatcgtg ggccagatgt
     1141 tgggccaggt gaagacgtcg ccattggtga cgttgaaggt ctcgttgcgc gcacctggcg
     1201 tgtctccggc ccaggcaatg gcgcgcgcca gcagatcggc gtcgacagcc tggctcactc
     1261 gctccgcgcc gccggggtag ggcaggatgg ggcctcgttc ccgcatcact gccgcatggg
     1321 cgccgagtgc cgggatcagg ttcatggcgc cgccgatcgc ctcaccgacg atcagaaccg
     1381 ggcggaggat agtgaacgtc caatccttcc cggcctgggc ggcacgcaga tagtcctcct
     1441 gcgtccagta gaaattcggc tgttcccgca tttccgagcg gccttcgcgg gcagggacgg
     1501 ccagcggacg gacatgaacg ccataggctt tggtaccctg aagcagggtt acgtgccgca
     1561 gccccggggc gacctgcagc agcggaccca gcaggttgcg cagcatgcgg tcgttggtgc
     1621 tgatctgctg gtcgttacgc caaccttcca ccaggcccgg cagctcgaac aaggcggcat
     1681 agaccacatg ggtcacgtcc cgcagcgttt ccgccgcgtg ccggcaagcc accgggttgg
     1741 tcaggtcgag tgccagatgc cgaacttcga ttcccggagg caggcggcgg gagacggcga
     1801 caacatcggt atcgggcgct gcggcgaagt gtttcaaggc cgcgtatccc acaaggcccg
     1861 ttgcacccgc caccagcacg gttttgcgac ccatcggcat caccctcctg gcgggaggat
     1921 atcaccgctc atgccagcaa gaccaatcga ctgatgcgat gtctcgggcg gaaagggcgt
     1981 acttcaagcc cgaaattgaa taggaagcag ccgattggca caatccgcct gggcgctggg
     2041 cacgatcagg tcatcgaact ccagaaaacg cgcggccgcg gcgatggctt gtgtggttgc
     2101 gtttgcaaag ccgtgccggc gcgatgccca taggaaccag ttgggccaca tcaacgaagc
     2161 gcaaggtcaa gttcggctgc gcgttaagcc gaggaatctc atgccgcgca tgcagcggcc
     2221 tatctaaacc acggccggcg ttatgcaatg gaacaggcgg cctgatgcat gacagctttc
     2281 gggttgaa
//
LOCUS       DASWQN010000554         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_185736, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000554 DASWQN010000000
VERSION     DASWQN010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185736"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VGC82_09270"
     CDS             <1..521
                     /locus_tag="VGC82_09270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423497.1"
                     /translation="DIKNSALFASGVFFAGVVGDSLGGILSDGIFRRTGNVRFARLSV
                     IILGFAGAFLSLLPMLFVHDITVVALCLSSGFFFAELVIGPIWSVPMDIASKYSGTAA
                     GLMNSGSALAAIVSPLVAGYVIDVTGNWYLPFLMSMGLLLVGGFSAFLMHPEKPFEDL
                     GPAPVGQPVTVG"
     gene            522..>1690
                     /locus_tag="VGC82_09275"
     CDS             522..>1690
                     /locus_tag="VGC82_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0423498.1"
                     /translation="MAAVPEQWRGRGLRLLSDKGTRRSKSVIGAPRSQGFSGEGRYMT
                     VHTGRHFLQIPGPTNIPDRVLRAMDMPTMDHRGAEFAQLGFDVLAASQRVFRTKQPVI
                     IYPSSGTGAWEAAIVNTLSPGDKVLMAETGQFAVLWCGIADKFKLDVDFLPGNWRRGA
                     DVEQIEARLAADRQHLIKAVMVVHNETSTGCVTHPLDVRKAMDRVRHPALLMVDTISG
                     LGSLEYEHDAWGIDVSVAGSQKGMMLPPGLGFNAVSEKALAVAQANPSMRSYWDWQEV
                     VAINKVGTWPYTPAVNMLFGLKEAIAMLEEEGLENVFARHKRHSAATRAAVKAWGLET
                     QCQDPHAHSPALTGVVMPEGHDADNFRKVVLENFDMSLGTGLNKIKGKVFRIGHIG"
BASE COUNT          296 a          477 c          567 g          350 t
ORIGIN      
        1 tggacatcaa gaattcggcg ctgttcgcct cgggcgtttt cttcgccggg gtcgtcggcg
       61 acagccttgg cggcatcctt tcggacggga ttttccgccg taccggcaat gtgcgatttg
      121 cgcggctcag cgtgataatc ctcggttttg ccggtgcctt cctgtcgttg ctgccgatgc
      181 ttttcgtgca cgacatcacc gtcgtcgcgt tgtgcctgtc gagcggcttc ttctttgcgg
      241 agctggtgat tggcccgata tggtccgtgc cgatggatat tgcgtcaaag tattccggga
      301 cggcggccgg cctgatgaac tcaggctcgg cgctggcggc gatcgtttcg ccgctggtgg
      361 ccggttacgt gatcgatgtc accggcaact ggtacctgcc gttcctgatg tcgatggggt
      421 tgctgctcgt cgggggcttc tcggcgttcc tgatgcatcc cgaaaagccg ttcgaagacc
      481 tcggtcccgc tccggtcggc cagccggtca cggtggggtg agtggcggcg gtcccggaac
      541 aatggcgggg ccgcggactc aggctcctct cagacaaggg gacaaggcgg tcaaaaagtg
      601 ttatcggggc cccgcgatcg caaggattct cgggggaagg acgctatatg actgtgcata
      661 ctggaaggca ttttctccag attccaggac cgaccaatat acccgaccgg gtgctccggg
      721 cgatggacat gcccaccatg gatcatcgcg gtgccgaatt cgcccagctt ggctttgacg
      781 tgctggcggc ctcgcagcgc gttttccgca ccaagcagcc ggtgatcatc tatccgtcgt
      841 cggggaccgg tgcgtgggaa gccgcgatcg tgaatacgtt gtcgcctggc gacaaagtgc
      901 tgatggccga gactggccaa tttgcagtgc tgtggtgcgg catcgccgat aaattcaagc
      961 tggacgtcga tttcctgccg ggcaactggc ggcgcggggc cgacgtggag cagatcgagg
     1021 cgaggcttgc agccgatcgc cagcatctga tcaaggcggt gatggtcgtg cacaacgaga
     1081 catcgaccgg ctgcgtcacc catccgctcg atgtccgcaa ggctatggat cgcgtcaggc
     1141 acccggcgct cttgatggtc gataccattt ccggcctcgg ttctctggaa tacgagcatg
     1201 acgcctgggg catcgatgtg tccgtcgccg gttcgcaaaa ggggatgatg ctgccgccag
     1261 gcctcggctt caacgcggtg tcggaaaagg cgctggcggt ggcgcaggcc aacccgtcga
     1321 tgcgatcata ttgggattgg caggaagtcg ttgcgatcaa caaggtgggc acgtggccct
     1381 acacgccggc ggtcaacatg ctgtttggac tgaaagaagc cattgccatg ctcgaggaag
     1441 aggggctcga gaacgtcttc gcgcggcaca agcgccacag cgcggcgacg cgcgcggcgg
     1501 tgaaagcgtg gggactggaa actcaatgcc aggatccaca tgcgcattcg cctgcgctga
     1561 ccggcgtggt gatgccggaa ggccatgacg ccgacaattt ccgtaaagtg gtgcttgaga
     1621 atttcgacat gtcgctgggc acgggcctga acaagatcaa gggcaaggtg ttccggatcg
     1681 gccatatcgg
//
LOCUS       DASWQN010000555         4872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1769255, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000555 DASWQN010000000
VERSION     DASWQN010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769255"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGC82_09280"
     CDS             complement(<1..266)
                     /locus_tag="VGC82_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthetase family
                     protein"
                     /protein_id="HEY0423499.1"
                     /translation="MAGRKNNTLKAWSRHLPAILGVALLVGALYVLQREFRHLKLKDI
                     GEALSAIPATALVFSFVWTIVSYFILTFYDRLGTIYAGHKVSYGR"
     gene            complement(270..1847)
                     /locus_tag="VGC82_09285"
     CDS             complement(270..1847)
                     /locus_tag="VGC82_09285"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003628327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY0423500.1"
                     /translation="MSDTARNVLPEIPKAWPFEEARKVARRLAASGRDNALFETGYGP
                     SGLPHIGTFGEVARTTWVRHAFTELTGLPSKLLAFSDDMDGLRKVPDNIPNRDMLTPY
                     LGKPLTQIPDPFGTHDSFGAHNNARLRAFLDSFGFDYEFASSTEYYKAGRFDAALRRI
                     LECYEQVMAVILPTLGPDRRATYSPILPVHPHTGIVMQVPMQHIDPAGGTVTWKDPST
                     GDTFQTSVKGGACKLQWKADWAMRWYALQVDYEMSGKDLIDSVKLSARICRILGGQPP
                     EGMTYELFLDQDGQKISKSKGNGLSVQEWLTYAPNESLGQFMYNQPQRAKRLYFDVIP
                     RAVDEYLGNLDKLGTQPDRANPAWHIHGGRLPNHAGSPISFGMLLNLASVVNADQPDI
                     LWGFIKRYVPGATPDTQPLLARLADHAVAYYRDFVRPEKRYREPTAMERAALQDLATT
                     LAALPDDNAETIQNAVFEVGKRHPFPDLRSWFGCLYQVLLGQQEGPRFGVFVALYGIQ
                     ETITLVHAALARRADAA"
     gene            1964..2710
                     /locus_tag="VGC82_09290"
     CDS             1964..2710
                     /locus_tag="VGC82_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0423501.1"
                     /translation="MTIPLPAALSMEGKRALVTGAASGIGRATALVLAQLGAELLLCD
                     RAPMDATCDEIQAGGGKCATAQGDLTDDAFMGSLFAGGRIHAVAHCAGILEGRPWTED
                     QGWHARFHRVMDVNVRVPLQLAALATDHMAAHGGGHIALVGSVAGKTGGTSLTTPPDY
                     SASKGAVHALVKWLSRNAVGRGVLINAVAPGPVETPMTRGFNLGPTLPMGRIGRPEEL
                     AWPIAFLCTSAASYLSGAILDVNGGAFVGP"
     gene            complement(2736..3404)
                     /locus_tag="VGC82_09295"
     CDS             complement(2736..3404)
                     /locus_tag="VGC82_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423502.1"
                     /translation="MQRILGLTLAATLALSLPAIAAPERERIRGTVTAVGADTLAVHT
                     TNGDDVSIGLGGSTRYLQVVKASLADVGTDSYIGTATKTVGSTLVALEVLIFPPAMKG
                     TGEGHYAWDSIQDTTVAGGARTASMMTNGSVAAPAVASTMTNGSVAAASSKDGTRELT
                     VTYKDGQQTILVPPTAPIVTFKPGAMSDVTKGASVFINAVKDEGKITANAVAVGMDGM
                     KPPM"
     gene            complement(3489..4418)
                     /locus_tag="VGC82_09300"
     CDS             complement(3489..4418)
                     /locus_tag="VGC82_09300"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0423503.1"
                     /translation="MPVDPQIQTLLNLRAALPPLHTLSVADARAQFAARDFPGLRKPD
                     VASVVNRDMQGPAGSLPLRIYTPLGKGPFPLMVFFHGSGFVVCSLDTHDGMCRNLCAG
                     TGCLVVSVDYRLAPEHKFPAGADDCLAATRWAVANAGALGADPGRVFVAGDSAGGNLA
                     AVTALRIRDEGGPKLLGQLLIYPVTDYYDPGTASMVENADGYGLTRQGMIWFWDHYLA
                     DPSDGANPYASPYRAADLGGLPPALVVTAEYDPLRDEGENYADRLRQAGVPTEMKRWD
                     GMNHGFFFWPGVVDRAGAAMDEACGWVRSVLAS"
     gene            complement(4420..>4872)
                     /locus_tag="VGC82_09305"
     CDS             complement(4420..>4872)
                     /locus_tag="VGC82_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY0423504.1"
                     /translation="AGNASQLSDGASAAVLMEEAEASRRGLQPLGIYRGMAVAGCAPD
                     EMGIGPVFAVPKLLAKHGLTVQDIDLWELNEAFACQVLYCRDRLGIDPDKLNVNGGAI
                     SIGHPYGMSGARMTGHALIEGKRRGAKFVVVTMCVGGGMGAAGLFEVA"
BASE COUNT          855 a         1583 c         1576 g          858 t
ORIGIN      
        1 cgaccgtagg aaaccttgtg cccggcgtag attgtcccga gccgatcgta gaacgtaagg
       61 atgaaatacg agacgatcgt ccacacaaaa gagaacacga gggccgtggc cggaatcgcc
      121 gacagcgctt cgccgatgtc cttcagcttc aggtgacgga attcgcgctg caggacatag
      181 agtgcgccga ccagcagggc gacaccaagg atcgccggaa ggtggcggct ccaggctttc
      241 agggtgttgt tcttcctccc ggccatgctt caggccgcgt ccgctcgtcg ggcaagagcc
      301 gcatgcacca gcgtgatcgt ctcctggatg ccgtagagtg cgacaaaaac gccgaagcgc
      361 gggccttcct gctggcccaa caggacctga tacaggcagc cgaaccagct tcgcaggtcg
      421 gggaacgggt ggcgcttgcc gacttcgaag accgcgttct ggatggtctc ggcgttgtcg
      481 tcgggcaacg ccgccagcgt cgtggcgaga tcctgcagcg ccgcacgctc catggccgtc
      541 ggctcgcggt aacgcttctc cggccggacg aagtcgcgat aatacgccac ggcatgatcg
      601 gcgaggcggg ccagcaacgg ctgcgtgtcc ggcgtggccc ccgggacata ccgcttaatg
      661 aaaccccaca agatgtcggg ctggtcggcg ttcaccacac tggcaaggtt cagcagcatg
      721 ccaaaggaga tcgggctgcc ggcatggttc ggcagccgac cgccgtgaat atgccaggcc
      781 gggtttgccc gatccggctg ggtccccaac ttgtcgagat tgccgaggta ttcgtccacg
      841 gcgcgcggga tcacgtcgaa atacagtcgc ttcgcgcgct gcggctggtt gtacatgaac
      901 tgacccagcg actcgttcgg agcgtaagtc agccattcct gcaccgacaa cccgttgccc
      961 ttcgacttgc tgatcttctg gccgtcctga tcgaggaaca actcgtacgt catgccctcg
     1021 ggcggctggc cgccaaggat gcggcagatg cgggccgaca gcttcaccga atcgatcagg
     1081 tccttgccgg acatctcgta atcgacctgt agcgcatacc agcgcatcgc ccaatcggct
     1141 ttccactgca gcttgcaggc accgcccttg accgaggttt ggaacgtgtc cccggtggac
     1201 ggatctttcc aggtcaccgt tccgccggcc gggtcgatgt gttgcatcgg cacctgcatg
     1261 acgatgccgg tgtggggatg caccggcagg atcggcgaat atgtggcacg ccggtccggc
     1321 cccaaggtgg gaaggataac tgccatgacc tgttcgtagc attccaggat gcgccgcagc
     1381 gcggcgtcga agcggccggc tttatagtat tcggtggagg atgcgaactc gtagtcgaag
     1441 ccaaaggagt ccaggaacgc gcgcagcctg gcgttgttgt gcgcgccgaa gctgtcgtgg
     1501 gtgccgaagg gatcgggaat ttgcgtcagc ggcttgccca ggtagggcgt cagcatgtcc
     1561 cggttcggga tgttgtccgg cacctttcgc aaaccgtcca tgtcgtcgct gaacgccagc
     1621 agcttcgacg gcaggcctgt cagttcggtg aacgcgtgcc tgacccaggt ggtgcgtgcc
     1681 acttcgccga acgtgccgat atgcggcagc ccggacggtc cgtatccggt ctcgaacagc
     1741 gcgttgtccc tgccagaggc ggccaggcgg cgtgccactt tccgagcttc ctcgaacggc
     1801 cacgccttcg ggatctcagg caacacgttg cgcgctgtat cggacatggc cggaagctat
     1861 ggaccgggct gcgtcaggtc aatcgcaacc atcgtcttcc tccacgatgg acagaaattc
     1921 atccaggcgg gaacattgcc gacgataacc agaggaaacc gtgatgacga tacccctgcc
     1981 cgccgcgctg tcgatggaag gcaaacgcgc gttggtaacc ggcgccgcga gtggcatcgg
     2041 ccgcgccacc gcactggtct tggcgcaact cggtgcggag ctgctgcttt gcgatcgcgc
     2101 cccgatggac gcgacttgcg acgagatcca ggccggcggg ggcaaatgcg ccacggcgca
     2161 gggcgacctg accgacgatg cgttcatggg ctcgttgttc gcgggcggcc ggatacatgc
     2221 cgtggcgcat tgtgccggta ttcttgaagg gcgtccctgg accgaggacc agggctggca
     2281 tgcgcgattc catcgggtca tggacgtcaa cgtacgggtg ccgctgcaac tcgcggcgtt
     2341 ggcgacggac cacatggccg cgcatggcgg tggtcatatc gcactggtcg gatcagtcgc
     2401 gggcaagacc ggcggaacgt cgctgacgac tccgccggat tattccgcgt cgaagggtgc
     2461 ggtgcatgca ctggtcaaat ggctgtcccg gaacgcggtg ggacgcggcg tgctgatcaa
     2521 cgccgtcgcg ccaggtccgg tggaaacgcc catgacccgc gggttcaatc tgggaccgac
     2581 cttgccgatg ggccggatag ggcggcccga ggaactggcg tggccgatcg ccttcctgtg
     2641 cacctcggct gccagctacc tgtccggggc gatccttgac gtgaacggcg gggccttcgt
     2701 cggaccatga accaccgccg ttggatcggc caggcttaca tcggcggctt catgccatcc
     2761 atgccgacag cgacggcatt cgcggtgatc ttgccctcgt cctttacggc attgatgaat
     2821 acgctcgccc ctttcgtcac gtccgacatg gcacccggct tgaacgtcac gatcggcgcg
     2881 gtcggcggca cgaggatggt ctgctggcca tctttatagg tgacggtcag ctcccgcgtg
     2941 ccatccttcg acgatgccgc ggcgacgctg ccgttggtca tcgtgctggc caccgcgggc
     3001 gctgcgacgc tcccgttggt catcatgctg gctgtcctgg caccgccggc cacggtcgta
     3061 tcctggatgg aatcccaggc ataatggccc tctccggtac ctttcatggc aggcgggaag
     3121 atcaatacct cgagcgccac cagcgtcgag ccgacagtct tggtagcggt gccgatgtag
     3181 ctgtccgtcc cgacatcggc gaggcttgcc ttcacgacct gcaggtatct cgtgctgccg
     3241 ccaagaccga tcgagacgtc gtcgccgttc gtcgtgtgga ccgccagggt gtcggcgccg
     3301 accgccgtga cggtgccgcg gattcgctca cgctccggcg cggcgatggc cggaagcgat
     3361 agcgcgaggg tcgccgcgag cgtaagtccg agtatccgct gcatgatgat ttccttcgat
     3421 tggcgcacta cggcgggcgg ggaactttat gttccccgaa cgctggcgac gatgttctta
     3481 attcttgatc aggaagccag gaccgagcgt acccagccac acgcctcatc catcgctgcc
     3541 ccggcgcgat cgacgacgcc aggccagaaa aagaacccat ggttcatgcc gtcccaccgc
     3601 ttcatctcgg tcggcacccc agcctgccgc agacggtcgg cgtagttctc gccctcgtca
     3661 cgcagaggat cgtactccgc tgtcacgacc aaggcaggag gcaacccgcc caggtccgcc
     3721 gctcggtacg ggctggcgta cggattcgcg ccatctgacg ggtcggccag ataatggtcc
     3781 cagaaccaga tcatcccttg gcgggtcagg ccgtagccgt cggcattttc caccatcgag
     3841 gccgtcccgg gatcatagta gtcagtcacc ggatagatca gcagttggcc cagcagcttc
     3901 ggcccgcctt catcgcggat ccgcaaagcg gtgacggccg ccagattgcc gcctgcactg
     3961 tcgccggcca cgaagaccct gccgggatcg gctcctaaag cgccggcgtt cgccaccgcc
     4021 caccgcgttg ccgccaggca gtcatccgct ccggcgggga atttatgttc gggtgcgagg
     4081 cggtaatcca ccgagaccac gagacagccg gtgccggcac acaggttgcg gcacatgccg
     4141 tcatgcgtat ccaggctgca caccacgaag ccgctgccat ggaagaatac catcagcggg
     4201 aacggacctt tgcccaacgg cgtgtagatc cgcaacggca gcgacccggc cgggccctgc
     4261 atgtcgcggt tgacgacgct ggcgacgtcc ggcttgcgca agcccgggaa atccctggca
     4321 gcgaactggg cgcgagcatc ggccaccgac agcgtatgca gcggcggcaa ggccgcccgc
     4381 aggttcagca gcgtctggat ctggggatcg acgggcatgt caggcgacct cgaacaagcc
     4441 ggccgcaccc atgccgccgc cgacgcacat ggtgaccacg acgaacttcg ctccgcggcg
     4501 cttgccctcg atcagtgcgt gcccggtcat ccgggcgccc gacatgccat acggatggcc
     4561 gatcgagatc gcgccgccgt tgacgttcag cttgtccgga tcgatgccca gccggtcgcg
     4621 gcaatacagc acctggcagg cgaaggcctc gttcagctcc caaaggtcga tgtcctggac
     4681 agtcaggccg tgcttggcca gcagcttggg tacagcgaac accgggccga tgcccatttc
     4741 atccggcgcg cagccggcga ccgccatgcc gcgatagatg cccagcggtt gcagcccacg
     4801 gcgggaggct tcggcttcct ccatcagcac cgcagccgag gcgccgtccg acagctgcga
     4861 cgcgttgccg gc
//
LOCUS       DASWQN010000556         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1769345, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000556 DASWQN010000000
VERSION     DASWQN010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769345"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1928)
                     /locus_tag="VGC82_09310"
     CDS             complement(228..1928)
                     /locus_tag="VGC82_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEY0423505.1"
                     /translation="MIDDLWYKNGVFYCLSVGTYMDANGDGIGDFKGLLRRLDYLNGL
                     GITTIWLMPFQPSPGKDDGYDISDYYGVDPKYGTLGDFVEFTHGCAQRGIRVIIDLVV
                     NHTSDQHPWYREARRAKDSPYRDWYVWSDKKPANADKGVVFPGVQKSTWTRDPEAKAW
                     YFHRFYDFQPDLNTSNPHVQAEILKIMGFWIQLGVSGFRMDAVPFVIATKGPNVRKPI
                     EQYDKLRSFREFLQWRQGNAIILGEANVLPKTDMEYFGKDGERMQVMFNFHVNQHLFY
                     AMASADCRPLAKALVQTKPRPATAQWGLFLRNHDELDLGRLTKAQRQLVFERFAPDKS
                     MQLYDRGIRRRLAPMLGGDRRRLELAYSLMCTLPGTPVIRYGDEIGMGDDLDLPERNC
                     ARTPMQWSTEPHAGFTESDRPCSPVIAEGPYSFEHVNVAKQRRDPNSMLNWTERIIRM
                     RKEVPEVGWGDFKVITTRDPAVLIIRYDWRNNSVLFVHNLDEKPREISFAAGIPGDPG
                     KLLVNLLSEDHSQADGRGKHKLLLEAYGYRWYRVGGLDYLLKRSDIDTGPAGKAGHPA
                     "
     gene            2234..>2439
                     /locus_tag="VGC82_09315"
     CDS             2234..>2439
                     /locus_tag="VGC82_09315"
                     /inference="COORDINATES: protein motif:HMM:NF018070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEY0423506.1"
                     /translation="MEWPAHKISLLPPPDRRQSETALLIARGTARLLRSLGFSCISEL
                     PLPSGRRADLVALNERGEIWIVEI"
BASE COUNT          427 a          748 c          789 g          475 t
ORIGIN      
        1 cgcgacaatg aaggcgtgat catcggccaa taagccagcg tcgtcccggc caagccaacg
       61 ggtccggcct tcggccggcc cgatgacagg ctccgcgcga gccgggatcc ataaccgccg
      121 atgtccggtc taggaaaagc cggaactcca gccgacgcga caacaaatat cggtggttat
      181 gggtccctgc tttcgcaggg gcgacgtgga caggccacgg catgcactca cgccggatga
      241 ccggccttgc ccgccgggcc ggtgtcgatg tcgctgcgtt tgagcagata gtcgaggcca
      301 ccgacgcggt accagcgata gccgtaggcc tccagcagca gtttgtgctt gccgcgcccg
      361 tcggcctggc tgtggtcctc gctcagcagg ttgaccagaa gcttgccggg gtcgccggga
      421 atgcccgccg cgaacgagat ttcgcgtggc ttctcatcga gattgtgcac gaacaacacc
      481 gaattgttgc gccagtcgta acggatgatc agcaccgccg ggtcgcgcgt ggtgatcacc
      541 ttgaaatcgc cccagccgac ttccgggacc tccttgcgca tgcggatgat gcgttcggtc
      601 cagttcagca tcgagttggg atcgcggcgc tgcttggcga cattgacatg ctcgaagctg
      661 taggggcctt ccgcgatgac gggggagcat ggtctgtcgc tctcggtgaa acccgcatgc
      721 ggttcggtcg accattgcat cggtgtgcgc gcgcaattgc gctcgggcag atcgagatcg
      781 tcgcccatcc cgatttcgtc gccatagcgg atcacgggcg tcccgggcag cgtgcacatc
      841 aggctgtagg cgagttcaag ccgccgccgg tcgccgccca gcatcggggc cagccgccgc
      901 ctgatgccac gatcgtagag ctgcatgctc ttgtcggggg caaatctttc gaacaccaat
      961 tggcgttgcg ccttggtcag ccggccgagg tcgagttcgt cgtggttgcg caggaacagc
     1021 ccccattgcg cggtcgccgg cctcggtttt gtttgcacca gcgccttggc cagcggacgg
     1081 caatcggccg acgccatcgc atagaacaga tgctggttga cgtgaaaatt gaacatcacc
     1141 tgcatgcgct cgccgtcctt gccgaaatat tccatgtcgg ttttgggcag cacattggct
     1201 tcgccgagaa tgatggcgtt gccttgccgc cattgcagaa actcccggaa cgaccgcagc
     1261 ttgtcgtatt gctctatcgg ctttctgaca ttcggtcctt tggtggcgat cacgaagggc
     1321 accgcatcca tccggaatcc ggacacgccg agctggatcc agaatcccat gattttcagg
     1381 atctccgcct gcacgtgcgg gttcgaggta ttgagatccg gctggaagtc atagaagcgg
     1441 tggaagtacc aggccttggc ttccgggtcg cgcgtccatg tggatttctg cacgcccgga
     1501 aacaccacgc ccttgtcggc attggcgggc ttcttgtcgg accagacgta ccagtcgcgg
     1561 tagggcgagt ccttcgcgcg gcgcgcttcg cgataccagg ggtgctggtc cgaggtgtga
     1621 ttgaccacga ggtcgatgat gacgcggatg ccgcgctgtg cgcagccatg ggtgaattcg
     1681 acgaagtcgc cgagcgtgcc gtatttggga tcgacgccgt aatagtcgga gatgtcgtaa
     1741 ccgtcgtcct tgcccggcga gggctggaac ggcatcagcc agatcgtggt gatgccaagg
     1801 ccgttcagat agtccagccg gcgcagcagg cccttgaagt cgccgatgcc gtcgccattg
     1861 gcatccatgt aggtgccgac ggagaggcaa taaaagacgc cgttcttgta ccagaggtcg
     1921 tcgatcatgc aggcagccca tatcggtccg ccccggtatt cggcggccgt gtgataagcg
     1981 gcaaaggctt tgcgggttcc cccggagggg acgcagcggt cgcgcggtca gcccgcccgg
     2041 tctgggggct tatccttcga gacgcatcgc tcgcgcgatg ctcctcagga tgaggtctta
     2101 gaccctcatg gtggggagcg cggcaagccg cgcgtctcga accacgatgt cccgtcgtgc
     2161 gaaaccggtg gcgggatatg acagcactgt tttccacctt cagcccatcg ccggaatcgg
     2221 atagaccacc gtcatggaat ggcccgccca caagatcagc ctgctgccgc cgccggaccg
     2281 ccgtcagtcg gaaacggcgc ttctgatcgc gcgcggcacc gcgcggctgt tgcggtcgct
     2341 gggattttcc tgcatcagcg aattgccgct gccttcgggg cgccgcgccg acctggtggc
     2401 actgaacgag cgcggcgaga tctggatcgt cgagatcaa
//
LOCUS       DASWQN010000557         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1637323, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000557 DASWQN010000000
VERSION     DASWQN010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637323"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VGC82_09320"
     CDS             complement(<1..608)
                     /locus_tag="VGC82_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0423507.1"
                     /translation="MPHLTQAELAHGPGAAPGALAAAVPLLELSGIGKSFGAVRALDG
                     MDLAIGHAEVVGLMGDNGAGKSTLTKIIAGNFPPSQGQIRLEGAPVTFDRPADARNAG
                     IEVMYQDLAIAENLTAAANVFLGRELHRRIGPLRVLDHRAMNRRAAELFRELKSETRA
                     EDTVREMSGGQRQAVAIARTRLAKSKLVLMDEPTAAISVRQVA"
     gene            866..1761
                     /locus_tag="VGC82_09325"
                     /pseudo
     CDS             866..1761
                     /locus_tag="VGC82_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          312 a          647 c          689 g          354 t
ORIGIN      
        1 gccacctggc gcaccgagat ggcggccgtc ggctcgtcca tcaggaccag cttggatttc
       61 gccagccggg tgcgcgcgat cgccaccgcc tggcgctggc cgcccgacat ctcccgcacg
      121 gtgtcctcgg cacgggtctc acttttcagt tcgcggaaca gctcggccgc ccggcggttc
      181 atcgcccggt gatccagcac gcgcagcggt ccgatgcggc ggtgcagctc gcgccccagg
      241 aacacgttgg ccgccgctgt caagttctcg gcgatggcga ggtcctggta catcacctcg
      301 atccccgcat tgcgggcgtc tgccgggcgg tcgaaggtga cgggcgcgcc ctcgaggcgg
      361 atctgaccct ggctcggcgg aaagttgccg gcgatgatct tggtcagcgt ggacttgccg
      421 gcgccgttgt cgcccatcag gccgaccacc tcggcatggc cgatcgccag atccatgccg
      481 tccagcgccc gcaccgcccc gaacgacttg ccgattccgc tgagttcgag caggggaaca
      541 gccgcggcca gcgcacctgg cgcggcaccc ggaccgtggg caagctcagc ctgcgtgagg
      601 tgtggcactg atcctcccat cggttgtttc cgagttggcc atctgaaggc ctggtgactc
      661 tgcatgatgc gcagcgcgtc cacaatcacc gtcttgcagc ggcaccacct tggtttgatg
      721 tccgcgatta gtggaggctg tgtgagaacg gatcgtcgcg tccacgaccg cagcggagcc
      781 tcaacgcttt ggttccatga gttcgatgcg gtgggattga cccggttcga tggacaccct
      841 gaagctttca cgcgaggtgt tccttatgcc cagaagccgc ccaccctatt cccctgagtt
      901 ccggcgccag atggtcgatt tggtgcgtgc cggacgatct ccggacgacc tggcccgcga
      961 gttcgagccg acgtcgcatt caatccgcgc ctgggttgcg ctggccgaca ggcaggaggg
     1021 ccgccgggag gaagctgcac ctggtctggc cgcggccgag cgcgacgaac tggcacggct
     1081 gcggcgcgaa gtgcgccagc tccggctgga gcgcgacatc ctctcaaaag ccgcggcctg
     1141 gtttgcccgg gaaaccggga cgctgccgtc cgggtctttc ggttcatgag cgcaaaccag
     1201 gcctgtttcc cgattgcccc catggcgcgt gtgcttggcg tgtccaaagc aggctactac
     1261 gcttggctgc accgcccacc ctccgcccat gctgttgccg acgtggcctt gctgaaacgt
     1321 gtcaggactg tccacgccag ctcgcgccag acgtatgggg cgccgcgcgt ccatgccgat
     1381 ctgcgggcag gaggagagag gcacggacgc aagcggatcg cgcgcctaat gcgggacgcc
     1441 gggctggtcg gcgcctgtca tcggcgtggc gggcccgcca ccacccggcg cgacaaggaa
     1501 gcgcggcccg cgcccgacct ggtggaccgc aatttcacgg cggcgggacc gaaccagcta
     1561 tggatcgcag atatcaccta cctgccgact gcggcggggt tcctgtatct ggctgtcgtg
     1621 ctcgacgtgt ggagccgcaa ggttatcggc tggtccatgg cgaaccatct gcgagccgag
     1681 ctggtgctgg acgcgctcga tatggccgta ggccaacggc ggcccaagga cgtcgtacat
     1741 catagcgacc agggcagcca aggtagattc aaatggtcgt cgcaacactc tgatgcagga
     1801 gattgcgatg atccatcgaa agcggcgttc ggcacggttt gggcgggcgc cattggtatc
     1861 gccaggtcga ccgccggtgg cgggacggga ggaacagcgg cggttttggg cggggattgc
     1921 ggcaggtttg tcgagtgagg gtgctgcgat tggggccggt gtgcctcagg ctgtcggaac
     1981 acgatggttt cggaaggccg gc
//
LOCUS       DASWQN010000558         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_449616, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000558 DASWQN010000000
VERSION     DASWQN010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449616"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VGC82_09330"
     CDS             complement(<1..857)
                     /locus_tag="VGC82_09330"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0423508.1"
                     /translation="MTGAAASAQEAGNYPSRPVMMIVPFAPGGASDFVARIIQHGVSD
                     ILGQQIVIDNRPGAAGMIGTESAARAAPDGYTTFLGNVGTISINPGIYAANMRIKPDQ
                     DLSPVTICADTPSILITRLDFPANSVSELVTYVKANQGKVSFATPGSSTLNRLEMEVF
                     KKDAGLDMVHVPYKGGAGPAVTDVLAGHVDLMFTTISSAMQFVKENKVKALAVTTPER
                     MADLPDVPTMYELGWKNLVTSSWQGVLVPAGTPRPIVEKLHAAIVKVLADPAIQAHMR
                     NVGAIAVAS"
     gene            1247..1933
                     /locus_tag="VGC82_09335"
     CDS             1247..1933
                     /locus_tag="VGC82_09335"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY0423509.1"
                     /translation="MSAPLQIGLVIFPKVTQLDLTGPVQVFSNVPGAKLHLIWKRIEP
                     VPSDSVLVLTPTTTFADCPQLDVICVPGGAGTDDMVNDEELLEFVRRQAGHARYVTSV
                     CTGSLVLGAAGLLKGYRATTHWTAMDLLAPFGATPEKTRVCVDRNRITGGGVTAGIDF
                     ALTLVSIMVNRSTAELIQLGLEYNPAPPFNSGSPDTAPPEILALMKEKIAPRRARRIE
                     ANSRAAARLG"
     gene            complement(2293..>2691)
                     /locus_tag="VGC82_09340"
     CDS             complement(2293..>2691)
                     /locus_tag="VGC82_09340"
                     /inference="COORDINATES: protein motif:HMM:NF006040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEY0423510.1"
                     /translation="FLKFFTWWSGQTFGTQLWTWRFGELVGTDEQGNRYYRTIGGKID
                     PSLGFERRWVIYNGAVEASRIPPSWHGWMHHTVDVPPVAEAYSPREWEKPHLPNLTGT
                     PYAYRPSGSTLASGRRPEATGDYQPWTPGN"
BASE COUNT          445 a          869 c          880 g          497 t
ORIGIN      
        1 ttgctggcca ccgcgatcgc gccgacgttg cgcatgtgcg cctggatcgc agggtccgcc
       61 agcaccttga cgatcgcggc gtgcagcttc tcgacgatcg gccgcggcgt acccgcgggc
      121 accagcacgc cctgccagga cgaggtcacg aggttcttcc agccgagttc gtacatggtc
      181 ggcacgtccg gcaggtcggc cattcgctcc ggggtggtga cggcaagcgc cttgaccttg
      241 ttctccttga cgaactgcat ggccgacgag atcgtcgtga acatgagatc gacgtggccg
      301 gccagaacgt cggtcaccgc cgggccggcg ccgcccttgt agggcacatg caccatgtcg
      361 agccccgcgt cctttttgaa gacctccatc tccagccgat tgagggtact gctgccgggc
      421 gtggcaaagc tgaccttgcc ctggttcgcc tttacatagg tgaccagttc gctgacgctg
      481 ttggccggga agtccagccg ggtgatcaga atgctgggcg tgtcggcgca gatcgtgacg
      541 gggctcagat cctggtccgg cttgatgcgc atgttggcgg cgtagatgcc cgggttgatc
      601 gagatcgtgc cgacattgcc gagaaaggtc gtgtagccgt ccggcgccgc gcgggccgcc
      661 gattcggtgc cgatcattcc agccgcgccc ggccggttgt cgatcacgat ctgctggccc
      721 aggatgtcgc tgacgccgtg ctggatgatg cgggcgacga aatcggatgc gccacccggt
      781 gcgaacggca cgatcatcat cacgggtctg ctcggatagt tgccggcctc ctgcgcggac
      841 gctgccgccc ccgtcaccag caacaaggcg ctgctgagcg cggccgcgat ggcgtttcca
      901 attcgctgcc gtgatgacat ggtaagtccc cgccggagta gccggttcga atcctagtct
      961 agcgggaata tttgtgccac cccttttgca actggccaga gcgcgcggca tttccggtgc
     1021 gagtccgcgt tgtacgccat ggcggaggtg gaccgatcgt gacaggatgg cgcggtcaag
     1081 tggccggctc gagtccgcga gaatttgtac gggatcggca gccacacccc atcgtcgtgg
     1141 ccggcgtcat ccgggcatcc atcattcttc gcggagcggt ctttgcgaaa ccaacgggtt
     1201 cgggcaatga cgaatgcaag ttaacgcaca acgaaaggac ctcgcgatgt ccgcgccgct
     1261 tcagatcggc cttgtgatat ttccgaaggt gacccagctc gatctcaccg ggccggtgca
     1321 ggtgttttca aacgtgcccg gcgcgaagtt gcatctgatc tggaaacgga tcgagcctgt
     1381 gccgagcgat tccgtactgg tgctgacgcc caccacgaca tttgccgatt gcccgcagct
     1441 cgacgtgatc tgcgtgcccg gcggcgcggg caccgacgac atggtcaatg acgaggagtt
     1501 gctggagttc gtgcgccgcc aggccgggca cgccagatac gtcacctcgg tctgcacggg
     1561 gtcgctggtg ctgggcgcgg ccggtttgct caaaggctac cgcgccacca cccactggac
     1621 cgcgatggac ctgctcgcgc cgttcggggc tacaccggag aagacccgcg tctgcgtcga
     1681 ccgcaaccgc atcaccggcg gcggcgtcac cgcaggcatc gacttcgcgc tgacgctggt
     1741 gtcgatcatg gtgaaccggt cgaccgcgga gctgatccag ctcgggctgg aatacaatcc
     1801 ggcgccgccg ttcaactcgg gctcgccgga taccgcgccg ccggaaatcc tggcgctgat
     1861 gaaagagaag atcgcgccgc gccgcgcgcg ccggatcgaa gccaacagcc gcgccgccgc
     1921 ccggctcggc tgagctttct ggcgagatcg cacgccaaga aaaaggccgc ctggtttccc
     1981 agacggcctt tcctgtccta cggtggcacc acattgacgg gcgattcagg acatctgctc
     2041 cgggggccta tctcccagcc ctgtcctgat cgcgcgccgc tgcaaatcgg gtcagtcgcg
     2101 atctggcccc gaaccgtgca atggtttccc atctccgtaa gatgacccat cgagccatga
     2161 gagacttccg ctggcaatga tgccgcggcg gatgtccccg ctgttcccgt ttttcacagg
     2221 ggttgattgg ctcgtcatct cgagcttcgg ggttcaaccc tgacgggccg ccccggaaga
     2281 cagcctgaag cctcagtttc ccggcgtcca gggctgatag tcgccggtcg cctcggggcg
     2341 gcggccgctg gccagggtcg agcccgacgg acggtaagcg tagggcgtgc cggtcagatt
     2401 ggggaggtgc ggcttttccc attcgcgcgg gctgtaggcc tctgcgaccg gcggcacatc
     2461 gacggtgtgg tgcatccagc catgccatga cggcggaatc cgcgacgcct ccacggcgcc
     2521 gttgtagatc acccagcgcc gctcgaagcc gagcgacgga tcgatcttgc cgccgatggt
     2581 gcggtaatag cggttgccct gctcatcggt gccgaccagt tcgccgaacc gccaggtcca
     2641 caattgcgtg ccaaaggtct ggccgctcca ccaggtgaag aatttgagga a
//
LOCUS       DASWQN010000559         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_186095, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000559 DASWQN010000000
VERSION     DASWQN010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186095"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VGC82_09345"
     CDS             complement(<1..392)
                     /locus_tag="VGC82_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEY0423511.1"
                     /translation="MNHIDSAINIDDLKRMARRRLPKIMFDFIEGGVEDEVGLRTNAS
                     AFRNQRLVPRYYVDTSKREQRAKLLGRTYASPFGIAPTGMAGAFRRDAELFLAQAAAE
                     ADIPYLMSGASNASMEAGAKLAPKNLWY"
     gene            complement(516..1961)
                     /locus_tag="VGC82_09350"
     CDS             complement(516..1961)
                     /locus_tag="VGC82_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0423512.1"
                     /translation="MTKNRYPAALRKHLAAAVIMVVVGSMGGLHALAEDVGANKSDLI
                     RGLLPTVVNISVSKEEAAPPTATATVSASVPSADSFPTIKSFVGSGFIIDPSGLIVTN
                     YHVVENAFEITVTFADGTRRSGKTLNGSRTADLALVKVDTEQPLPAAHWGDSDVVKVG
                     DQVFAAGNPFGIGLSVSAGIVSGLNRDIQNSPYDDLIQTDATINHGNSGGPLFDMQGN
                     VIGVDSAIISPTTGSAGLGFALPSNTARFVIGQLQTYGWVRPAWIGVKVQRVTPPMAD
                     ALGMPQPEGSIVAWVLPAGPAHKAGLLIGDVILRFDGKAPSDDRALLRDIARSQIGST
                     LTLLVRRNDQERIIPITAEVWPRDQWDARDAPLLAQRPRIVIPPDLGLSLTELAKDQK
                     AAAGLPDGLDGVLVTKIPANSDPAHQGMASGDIILRVQDKPVATPTEVQAGIDAARAE
                     KREHVLMLVLPKVRDVPGPKWVALQLGPDGA"
BASE COUNT          380 a          708 c          669 g          414 t
ORIGIN      
        1 tgataccaca aattcttcgg cgccagcttt gcgcccgctt ccatcgacgc attcgacgca
       61 cccgacatca aatagggaat gtccgcctcg gctgccgcct gcgccagaaa cagttcggca
      121 tcgcggcgga atgcgcccgc catgccggtg ggtgcgatgc cgaacgggct ggcgtaggtg
      181 cggcccagca gcttcgcgcg ctgctcgcgc ttcgaggtat cgacgtagta gcgcggcacc
      241 aggcgctggt tgcggaaggc gctggcattg gtgcgcaggc ccacctcgtc ctccacgccg
      301 ccttcgatga agtcgaacat gatcttcggc agccggcgcc tggccatgcg cttgaggtcg
      361 tcgatgttga tcgcgctgtc gatgtggttc atgtgcgttc gatccctttg ggctcggcgc
      421 tgaacttatc cgcgctgcgt ggtccccgct atgatccggc ttccgtggct tcagccacga
      481 tggcgagcga ggctacattc catcccgaaa gtagatcatg cgccatccgg cccgagttgt
      541 agtgctaccc atttgggccc cggcacatcg cgaaccttcg gcagcacgag catcagcaca
      601 tgttcccgct tttcagcccg agccgcatcg atgcccgcct gcacctcggt tggcgtggca
      661 actggcttat cctgcacccg caggatgatg tcgccgcttg ccatgccctg gtgcgccggg
      721 tcggaattgg ccgggatctt cgttaccagc acaccgtcca ggccgtccgg cagccccgca
      781 gctgccttct ggtccttggc gagttctgtc aacgaaagac ctagatcggg cgggatgaca
      841 atcctcggcc gctgtgccag cagcggcgcg tctcgcgcat cccactgatc gcgcggccag
      901 acttccgcag tgatcggaat aatgcgttct tggtcgttgc gccgtaccaa cagcgtcagc
      961 gtactaccga tttgcgatcg ggcgatatca cgcagcaagg cgcgatcgtc gctcggcgct
     1021 ttgccgtcaa accgcaggat cacatcgcca atcaagaggc cagccttgtg tgccggcccg
     1081 gctggcagga cccaggcgac gatcgagcct tccggctgcg gcattccgag ggcgtccgcc
     1141 atcggcggcg tcacccgttg cacctttacg ccgatccacg cagggcgaac ccacccgtag
     1201 gtttgcagtt gtccaatcac gaaccgcgct gtgttcgacg gcagggcaaa gccgaggccg
     1261 gcggacccgg tggtgggcga aatgatcgcc gagtccacgc cgatcacgtt gccctgcatg
     1321 tcgaacagcg ggccgccgga attgccgtgg ttgatcgtgg cgtcggtctg gatcaggtcg
     1381 tcgtagggcg aattctgaat gtcgcgatta agcccgctga cgatgccggc ggacaccgag
     1441 aggccaatcc caaacggatt gcccgccgcg aacacctgat cgccaacttt taccacatcg
     1501 ctgtcgcccc aatgcgccgc cggcaaaggc tgctcggtgt ccaccttcac caacgccaga
     1561 tcggcggttc gcgacccgtt cagcgtcttt ccggaccgtc gtgtgccgtc cgcgaatgtg
     1621 acggtgatct cgaacgcatt ctcgaccaca tggtaattgg tcacgatcag cccggacgga
     1681 tcgatgataa agccggagcc gacgaagctc ttgatggttg ggaagctatc ggccgacggc
     1741 actgaagcgc tgacggtcgc cgtcgcggtc ggtggcgcgg cctcttcctt gctgacggag
     1801 atgttgacca ccgtcggcaa cagaccgcgg atgaggtcgg atttgttcgc accaacatcc
     1861 tcggccaagg catgcaaccc acccatcgag ccgaccacga ccatgatcac ggcggcggcg
     1921 aggtgtttgc gcaaggcggc agggtagcgg ttcttcgtca tgggacgcca tccatcgttc
     1981 aggcagaagg gcagttcggt cgcgccgggg cagggcacgt tcctctccgc gaatatcgcg
     2041 agcgcaccga ggcaatcgct atgaccctgc gcacatacac gcagattgct tcgctgcgcg
     2101 cgcaatgaca atgggaacta tcagtcggtt ggtcacagga ccgcgcggcc ccgatttagg
     2161 gtcctcccga t
//
LOCUS       DASWQN010000560         5323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2561418, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000560 DASWQN010000000
VERSION     DASWQN010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2561418"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VGC82_09355"
     CDS             <1..576
                     /locus_tag="VGC82_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEY0423513.1"
                     /translation="NDLIWANVVNNYLRGAKPPAFDLLCWNSDGTRMARAAHGWYLRN
                     TYVENNLIVPGKIELKGEALDLRRIKLDAYAVGAEKDHIVPWEAAWRLTQLSGGKVRF
                     VLASSGHIAGIINPPGGKGAYRVNDAKTADGTPEEWRASCQVHEGSWWVDWARWLTDR
                     SGDRGEPPGLGSTAHPALYDAPGRYVLETAS"
     gene            complement(552..725)
                     /locus_tag="VGC82_09360"
     CDS             complement(552..725)
                     /locus_tag="VGC82_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423514.1"
                     /translation="MLRVEPADYFADRDVGSACNIIKRDHAAVPSWRFGTSHRDAAKA
                     SPRIVRQDAVSKT"
     gene            773..2284
                     /locus_tag="VGC82_09365"
     CDS             773..2284
                     /locus_tag="VGC82_09365"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0423515.1"
                     /translation="MVQTAALPTFSALYAFGDSLSDAGNLSIITSAAGATEPVSPPYF
                     KEQYGPISGNVFSNGPTWVQNLSLGLGLGTLAPSMAAGTDFAYGGAETGSTPQNKGDL
                     KLQAISLGAQLSQFQTGVPKPSATALYTLSIGSNDLLDILAAPGLTAQQQTTDLNAAV
                     ANEIAFVKQLVSGGAKNLVVLGIPDLGKTPDVSLGLVNGSNTPSAALTAEATQLASSY
                     NAALVSQLATIAAGSAVNVQVVDTFSLLNAAVADPGAYGLTNVTSPVWSGNFTSAGSG
                     TLTVTGAAAQDQFLYWDALHPTETGHQAIADLAEQQLSGAPVLTALDTTTGQPAAAQG
                     TPYTGPVAGPQQQYIAITQDNLNITASSPNWFIHSGGGTDGLAVSSGTNVLDGGAGST
                     FFSGGTGTDSFFVDIRSATANLWSTVTNFHATDAATLWGVTQADFKLSWLDGQGAPGA
                     TGLTLVTTAVGRPLVGLTLAGYTQADLTNGRLSVAFGTDAGSGSGFMNIHANA"
     gene            2594..3673
                     /locus_tag="VGC82_09370"
     CDS             2594..3673
                     /locus_tag="VGC82_09370"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEY0423516.1"
                     /translation="MNQIAITRTDTPKQPPADETLAFGKVFSDHMFLMNYETGKGWHN
                     PRIAPYAPFVLDPATCVLHYGQAIFDGLKAFRGQDGRIRLFRAGDHARRLNRSAHYLC
                     IPELDPAMVEESIRTLVEVDQRWVPAMAGTSLYIRPTVIATETFLGVHPSHSYLYYVI
                     AGPVGAYYKEGMNPVSILASDTYVRAVKGGLGAAKTAGNYAASLFGAEEAQKAGYTQV
                     LWLDGVEHKYLDEVGTMNIMLKIGDEVITPPLNGAILAGITRDSVLTLLRDWGLRASE
                     RPVSIDEVIAAARAGTLEMWGTGTAAVISPVGELGYKNERYTINGGRTGALTQKLYDT
                     IVGIQYGTAPDPHGWARPLGNRLGG"
     gene            4522..4779
                     /locus_tag="VGC82_09375"
     CDS             4522..4779
                     /locus_tag="VGC82_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423517.1"
                     /translation="MSELPTIVLVPFIFGFLIFALRFYSRESASSMTNPSFLLAVATM
                     LTAGAFLLLRVTDLLPPYGTIGFGLIGVILLGTAVTRMFML"
     gene            complement(4790..>5323)
                     /locus_tag="VGC82_09380"
     CDS             complement(4790..>5323)
                     /locus_tag="VGC82_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423518.1"
                     /translation="CGAAAPELAALPPTARAVVESDEYRLLSTTALEEALPFLRWALI
                     CERTGDRQHQAEALLQAAWAADDAAAMSEAAKLRSDVAALWEATSDVEVGLRRLDVLR
                     RAGQFESATAWAETLSGWEPNEVSRAIIGFQIKRIAQRDIGRHLISSALPPPSHAPHV
                     SHGKRTTTGFWSRLLQK"
BASE COUNT          972 a         1748 c         1659 g          944 t
ORIGIN      
        1 aacgacctga tctgggccaa tgtcgtcaac aactacctgc ggggcgcgaa gccgccggcc
       61 ttcgatttgt tgtgctggaa cagcgacggc acccgcatgg cccgcgcggc gcacggctgg
      121 tacctgcgca acacctacgt cgagaacaac ctgatcgtgc ccgggaagat cgaactgaag
      181 ggtgaagcgc tggaccttcg ccgcatcaag ctcgacgctt atgcggtcgg tgcggagaag
      241 gatcacatcg tcccgtggga ggcggcatgg cggctgacgc aactgtccgg cggcaaggtt
      301 cgctttgtgc tggcgtccag cggccacatc gccgggatca tcaatccccc cggcggcaaa
      361 ggagcctacc gggtcaacga tgcgaaaaca gcggacggga cgccggagga gtggcgcgcc
      421 tcgtgccaag tccacgaagg aagctggtgg gtggattggg cgcgctggct gaccgatcgc
      481 tcaggcgacc gcggcgagcc acccggtctc ggcagcacgg cacatcccgc attgtacgac
      541 gcgcccggcc gctatgtctt ggaaaccgca tcctgacgca cgatccgagg gctggccttg
      601 gcggcatcgc gatgcgaggt gccgaagcgc catgacggaa cagccgcgtg atcgcgcttg
      661 atgatgttgc aggccgaacc aacgtcccgg tcggcgaaat aatccgccgg ctcgacgcga
      721 agcacgaccc ttcctgtaac ggggtgtcga tacgtcacaa aggacttctt tcatggttca
      781 gaccgcggca cttcctactt tcagcgccct ctacgcgttc ggcgacagcc tgtccgacgc
      841 cggcaacctc tcgatcatca cctcggcggc cggggcgacc gaaccggtgt cgccgccgta
      901 cttcaaggag cagtacggcc cgatcagcgg caacgtgttc agcaacgggc cgacctgggt
      961 gcagaatctg tcgctcggcc tcggcctcgg cacgcttgcg cccagcatgg ccgcgggcac
     1021 ggacttcgcc tatggcggcg ccgagacggg atcgacgccg cagaacaagg gcgacctgaa
     1081 gctccaggcg atttccctcg gggcgcagct ttcgcagttc cagaccgggg tgccgaagcc
     1141 gtcggcgaca gcactttata cgctgtcgat cgggtcgaac gatttgctcg atattctcgc
     1201 cgcccccggt ctgaccgcgc agcagcagac caccgacctg aacgccgccg tcgccaatga
     1261 gatcgccttc gtgaagcaac tcgtcagcgg tggcgcgaaa aaccttgtgg tgctgggtat
     1321 ccccgatctt ggcaagacgc cggacgtcag cctggggctg gtcaatggca gcaacacgcc
     1381 atccgccgcg ctaaccgccg aagccacgca gcttgccagt tcgtacaacg ccgcgctggt
     1441 cagccagctc gccaccatcg ccgccggcag tgcggtcaat gtgcaggtgg tggacacgtt
     1501 ttcgctgctg aacgccgcgg ttgccgaccc cggggcgtat gggctgacga atgtgacctc
     1561 cccggtctgg agcggaaatt ttaccagcgc cggcagtggc acactgacag tcaccggtgc
     1621 cgcggcgcag gatcagttcc tgtactggga cgcgctgcat ccaaccgaaa ccgggcatca
     1681 ggcgattgcc gatctggccg agcagcagct cagcggcgcg ccggttctga cggcgctgga
     1741 taccaccacg ggccagccgg cggcggcaca ggggacgccg tacaccggac cggttgcggg
     1801 accacagcaa cagtatatcg cgatcactca ggacaacctg aacatcaccg cctcatcgcc
     1861 gaactggttc attcacagcg gcggcgggac ggacggactc gcggtcagca gcggcaccaa
     1921 cgtgctggat ggcggcgccg gctcgacctt cttctcgggc ggcaccggga cggacagctt
     1981 cttcgtggat atccgttcgg ccacggcgaa tttgtggagc acggtgacca atttccatgc
     2041 cacggacgcc gccacgctgt ggggggtgac acaggccgac ttcaagcttt cctggctgga
     2101 cggacaaggc gcacccggag caaccggcct cacgctcgtc accacggcgg tgggcaggcc
     2161 gctggtggga ttgacgctgg cgggctacac gcaggccgac ctgacgaacg gacggctttc
     2221 ggtcgcattc ggcaccgacg ccggcagcgg cagcggattc atgaacatcc acgccaacgc
     2281 gtgacggcgt tcatgccctg acggcgccca tccctgacgg cgtccatccc ctgacggcgg
     2341 atccgctgaa cgcggactgc cgccggcgtc ctgcctccga agttcctcgc attgtgcgcc
     2401 ggacgcgagg gaccagtagg cgactgaaac cagattgagc gtcctggctc ggaggttcgc
     2461 ccgtcctcgg aggttcgccc gtcatggcga acttctgaac caggacgcgg gaaagtctta
     2521 cgcttgcccg ctgcaatggc tttcgctgcc gtggtatcgt agtctgcctt cgcacccaga
     2581 taacgagacg cacgtgaacc agatcgcgat tacccggacc gatacgccga agcagccccc
     2641 cgccgacgag actctggcgt tcggcaaggt tttctccgac cacatgttcc tgatgaacta
     2701 cgaaacgggg aagggctggc acaacccacg catcgcgccg tacgcgccct tcgtgctgga
     2761 cccggcgacg tgtgtcctgc actacggcca ggccattttc gacgggctga aggctttccg
     2821 cggccaggat ggccggatcc gcttgttccg agccggcgat cacgcccgcc ggctgaacag
     2881 gtcggcgcat tatttgtgca tccccgaact ggatccggcg atggtcgagg agtccatccg
     2941 taccctggtc gaggtcgatc agcgctgggt tccggcaatg gcgggaacct cgttgtatat
     3001 ccgccccacc gtcatcgcca ccgagacctt cctgggcgtg catccgtcgc acagttatct
     3061 gtactacgtc atcgccgggc cggtcggcgc ttattacaag gaaggcatga atccggtcag
     3121 catcctggcg tccgatacct atgtccgtgc agtcaagggc ggcctgggtg cggcgaaaac
     3181 cgccggcaac tacgccgcgt ctctgttcgg tgccgaggag gcgcagaagg ccggctacac
     3241 ccaggtgctg tggctggacg gagtggagca caaatacctc gacgaagtcg gcacgatgaa
     3301 catcatgctc aagatcggcg acgaggtgat aaccccaccc ttgaacggcg ctatcctggc
     3361 gggcatcaca cgagactccg tattgaccct gctgcgcgac tgggggttgc gtgcgtcgga
     3421 acgcccggtt tcgatcgacg aggtcatcgc cgccgcgcgc gccggcacgc tggaaatgtg
     3481 gggcaccgga acggctgccg tgatctctcc ggttggcgaa ctcggctaca agaacgagcg
     3541 ctatacgatc aacggcggcc ggaccggcgc gctgacgcaa aagctgtacg acactatcgt
     3601 cggcatccag tatgggacgg cgcccgatcc tcatggctgg gcccgtccgc tgggcaaccg
     3661 cctgggcggt tagaacctga tttcggcctg cgcagtcagg ctgggaatgg ttcagagcgt
     3721 tttcgcgctg actggagaag atgccggcca gttcgccgcc tgacgccaat gacgccaata
     3781 tggatgtcat ggccgagctt ggatatcggt gtgatagccg tgcttggata tgagtgtcat
     3841 ggccgtgctt ggcgcggcca cccacgactt cctttgcgcc gcgcgagaaa acgtctgttt
     3901 ccagtcaccg tgagaccgct ccagcctggg caatatgcgg ccatgcggtg cgttcgcgct
     3961 tttttattct caacacggag aaacaacgag ttccacgaag ggccacggag cagggtcgtg
     4021 ccgcgacatc cggggcaaac ggaaggcacg ataggttggc gatcgatttt tcttcgactg
     4081 cctatgtgac ggacctctca tcgagatcca ggttgccaaa gctttaaagc gcgcctcgag
     4141 gtagtgccgc actgatccgg gtgcccccgc ggtccagtcg cagggatgcc gttgcatgag
     4201 caggaccaat cgccgacaag tcgtgccgtc caatggacgg actgccttcg gcgcaccgta
     4261 gagcgagact gctcacagcc gggaaaccca agccacagtg caacgcgacc cgcagcggca
     4321 ccatctccgt ggctcttcgc ggccttcgcg cctgcttcgt gttaaaaatt gaaaggacga
     4381 acacactcgc tcatcgcacg aggccccggg cagtgcttca accttgcgcc aagcggcttc
     4441 tccgtcgccc gaaattcttt tctcagccag gggaatacgt tatcacgcac acgaaccgac
     4501 cacatgtgag tattcccgcc gatgtcagaa ctgccgacca tcgtcctggt gccgtttatc
     4561 ttcggcttcc tgatcttcgc gttgcgcttc tattcccgcg aatccgcgtc gtccatgacc
     4621 aaccccagct ttctgttggc tgttgcaacg atgctgacgg ccggcgcttt tctgcttctg
     4681 cgggtcaccg acctgctgcc gccctacggc acgatcggct tcggcctgat cggggtgatc
     4741 ctgctcggta ccgccgtcac acgcatgttc atgctttaaa gcaagacgat catttctgca
     4801 gcaggcgcga ccagaaccct gttgtggtcc gtttgccgtg ggaaacatgc ggagcgtgcg
     4861 acggaggcgg caaggcactg ctgatcagat gccgcccgat atcccgctgg gcgatgcgct
     4921 tgatctgaaa cccgatgatc gcccgcgata cctcattcgg ttcccacccg gacaatgttt
     4981 cggcccacgc cgtggctgat tcgaactggc cggcgcggcg caggacatcc agccgccgca
     5041 gtcccacttc aacgtcgctg gtagcttccc acaaggccgc gacgtcacta cgaagcttgg
     5101 ccgcttcgct catcgccgcc gcgtcgtcgg ccgcccaggc cgcctgcagc aatgcctccg
     5161 cctggtgttg ccgatcaccg gtccgctcgc agatcagcgc ccatcgcaaa aatggcagcg
     5221 cctcctccag cgccgtcgtg ctgagcaggc ggtattcgtc cgactccacc accgctcgcg
     5281 cggtcggggg cagggcggct agctcagggg ccgccgcgcc gca
//
LOCUS       DASWQN010000561         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2428943, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000561 DASWQN010000000
VERSION     DASWQN010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2428943"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..585
                     /locus_tag="VGC82_09385"
     CDS             37..585
                     /locus_tag="VGC82_09385"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HEY0423519.1"
                     /translation="MQREKDFRAYQDLESNFEKRPSLWGEPIGDWGEGQVQLLEIPTK
                     EEIHDNIAGFWRPKNVLQAKGEHTYTYRLHWGPDIPKATQLARFSRTGVGAKGDDLKI
                     FVLDVVGEKLKSIDPQTIRGDVTAGKAEIRNVVTQPNPATGGWRLSFELSVKDKIPIE
                     LRASLMQGNDPLSEVWVYRWTP"
     gene            573..>2573
                     /gene="mdoH"
                     /locus_tag="VGC82_09390"
     CDS             573..>2573
                     /gene="mdoH"
                     /locus_tag="VGC82_09390"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEY0423520.1"
                     /translation="MDTLTGRPGPEIADGGSRQEFLPPESPMAMSEQALRAFQKRDGD
                     QEAGSGSAGLRRACILIGTAAMTIAGCYQMYEVLQVGGVTTLEWMVLCLFVLLFAWIA
                     FSFMSALAGFLVLLFRIKDPLGIDADAPLPSIDSRNAMLLPTYNEDPDRIMARLQAMY
                     ESMQQTGYGSQFDWFVLSDTTNPAIWIAEEKCFLRLRGELDADNIFYRHRPENVARKS
                     GNIEDWIKRFGAGYQHMIILDADSLMTGDTVVRLVAAMETNPRVGLIQTLPVIVNART
                     LFARLQQFSGRLYGPLIAAGIAWWHASEGNYWGHNAIIRVRAFAQDAGLPELQGRKPF
                     GGHILSHDFVEAALMRRGGWDIRMVATLGGSFEECPPSLLDFAARDRRWCQGNLQHLA
                     VLPARALHWVSRLHLLTGIGSYLTAPLWLLFLLLGILISLQAQFVRPEYFPKGFALFP
                     KWPAQDPILAAWVFVGTMALLILPKLLAYVLLLTQARTRRQFGGGLTVFAGVVIETFL
                     SGLIAPVMMIFQSTAVGEILLGRDAGWQVQRRDDGKWPVRELIRKYAAPTLFGAAMAV
                     SAYAVSLPLLLWMAPVIVGLLLSIPIAMLSSMSSDPNSLLLRTPEQAAPPPVLVRANE
                     LAEARDETVACPLLALRNDARLLDAHLRNLPGDRPRNRGQINP"
BASE COUNT          489 a          762 c          766 g          556 t
ORIGIN      
        1 accttcgccg atctcaaccc tcgcgggttc ggcctgatgc agcgtgagaa ggatttccgg
       61 gcatatcagg atcttgaatc gaatttcgaa aagcggccga gcctgtgggg tgagccgatc
      121 ggagattggg gcgaaggcca ggttcagctg ctcgaaattc ccaccaagga agaaatccac
      181 gacaatatcg ccggcttttg gcggccgaag aacgtcctgc aagccaaggg cgagcacacc
      241 tacacttatc gcctgcattg gggcccggat atcccgaagg cgacacagct ggcacggttt
      301 tcacgcacag gcgtcggcgc aaagggcgac gacctaaaga tattcgttct cgacgtggtc
      361 ggcgaaaaac tgaaatcgat cgacccccag accatccgag gcgacgtcac cgcgggcaaa
      421 gccgaaatcc gcaacgtggt cacccaaccg aacccggcga ctggcggctg gcgattgagt
      481 tttgaactct cggtcaaaga taaaattccg atcgaattgc gagcatccct gatgcagggc
      541 aacgacccgc tatcggaagt atgggtctat cgatggacac cctgacagga cgtccgggtc
      601 ctgaaatcgc tgacggcggt tcgcggcagg aattcctgcc gccggaatcg ccgatggcga
      661 tgtccgagca ggcgctgcgc gcctttcaga aacgggacgg cgatcaagag gctggttcgg
      721 gttcggcggg gcttcggcgc gcctgcattc tgatcggtac tgcggcaatg accattgccg
      781 gctgctacca gatgtatgaa gttctccagg tcggcggcgt tacgaccctc gaatggatgg
      841 tgctgtgtct gttcgtgctc ctgttcgcct ggattgcatt ttcattcatg tccgccctcg
      901 ccggctttct ggtgctgctg ttccggatca aggacccgct tggcatcgat gccgatgcac
      961 cgctaccatc gatcgacagc aggaatgcga tgctgttgcc gacctacaat gaagacccgg
     1021 atcggatcat ggcccggctt caggcgatgt acgagtcgat gcagcagacc ggatatggct
     1081 cacagttcga ctggtttgtg ctgagcgata cgacgaatcc tgccatctgg atagcggaag
     1141 agaaatgctt cctgcgcctt cgtggcgaac tcgatgccga caatatcttt tatcgtcatc
     1201 gcccggaaaa tgtcgccaga aaatccggaa atattgagga ctggatcaag cgcttcggcg
     1261 ctggctacca gcacatgatt atccttgacg ccgacagcct catgacgggc gataccgtgg
     1321 tccggctggt ggctgcaatg gaaacgaatc cccgcgtcgg tctgattcag acactgcccg
     1381 tcatagtcaa cgcgagaacc ctgttcgccc gcttgcagca attctcaggg cggctttacg
     1441 ggcctcttat cgccgcaggc attgcgtggt ggcatgcttc cgaaggcaat tattggggcc
     1501 acaacgcgat cattcgtgta cgcgccttcg ctcaggatgc gggcctgcct gaacttcagg
     1561 gccgaaaacc gttcgggggg cacattctca gccatgactt cgttgaggcg gccctgatga
     1621 ggcggggagg ctgggacatc agaatggtgg ccacgctggg cggcagcttc gaagaatgtc
     1681 cgccatcgtt gctggatttc gccgcccgcg accgtcgatg gtgccaaggc aaccttcagc
     1741 acctggcggt gcttccggcg cgcgccctgc attgggtatc acggctgcat cttctcaccg
     1801 gcatcgggtc ttatctcacg gcgccgctct ggctgttgtt tctcctgctc ggcatattga
     1861 tctccctgca ggctcaattt gtccggcccg aatattttcc aaagggattt gccctgtttc
     1921 cgaaatggcc tgcccaggat ccgattctcg ctgcctgggt ttttgtcggc accatggcat
     1981 tgctgatcct gcccaagctg cttgcttacg ttctgctcct gacccaggcc cgaacccgcc
     2041 ggcaattcgg tggaggactg accgttttcg cgggggtggt gattgagaca ttcctgtcgg
     2101 gcctgatcgc gccggtcatg atgatctttc aatccactgc cgtgggcgaa attctgctcg
     2161 gacgcgatgc cggctggcag gttcagcgcc gggacgacgg caaatggccc gttcgggaat
     2221 tgatccgcaa atacgccgcg ccgaccctct ttggcgccgc gatggccgtc agcgcctacg
     2281 ccgtgtcatt gccgctgttg ctttggatgg cgcccgtcat cgtgggtctg ttgctctcca
     2341 ttccgatcgc gatgctgtca tccatgagca gtgatccgaa ttccctgcta ttgcgaactc
     2401 ccgagcaggc ggctccgcca ccggttctgg ttcgcgcaaa tgaactggcc gaggcacgtg
     2461 atgagaccgt cgcatgccca ttgctagcgc ttcgtaatga cgcgcgcctg ttggacgcgc
     2521 atttgaggaa tttgcccggg gaccggccgc gcaaccgcgg ccaaatcaac ccg
//
LOCUS       DASWQN010000562         3488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1637690, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000562 DASWQN010000000
VERSION     DASWQN010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637690"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VGC82_09395"
     CDS             <1..462
                     /locus_tag="VGC82_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0423521.1"
                     /translation="HADEDADFGVASDTELHHEYSGLTPTTAPGAQTIRTGDLVSLLA
                     ERQPLVIDVLTYFWGRSIPGAVGLKHAGAGGSFSGSGQDRLRGKMAELTGGDLSKPIV
                     AVGWNSERFDGRNLAHRLVAMGYTNVYWYRGGREEWEVAGLPEADLAATDW"
     gene            complement(476..1147)
                     /locus_tag="VGC82_09400"
     CDS             complement(476..1147)
                     /locus_tag="VGC82_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0423522.1"
                     /translation="MSDPLVLRAVTRIYRGDAGDLPVLRGADLSVRAGEIVALVAPSG
                     AGKSTLLHVAGLLDRPDGGSVLIEGRDAGGLPDAARTAVRRDTIGFVYQFHHLLPEFT
                     ALENVVLPQMIAGRTRRAAEDRARLLLTTFGLAPRLGHLPGKLSGGEQQRVAIGRALA
                     NGPKLLLADEPTGNLDVATAEAVFAELLAIVRDQGVAALIATHNPDLAARMDRTVTLR
                     EGMLV"
     gene            complement(1140..2393)
                     /locus_tag="VGC82_09405"
     CDS             complement(1140..2393)
                     /locus_tag="VGC82_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY0423523.1"
                     /translation="MFGPFERMVAGRYLRARKGERFVSIIAIFSLVGIALGVATLIVV
                     TSVMSGFQTELVTRILGVNGHISVEAYAGQKLDDYQPLVSQIRAIKDVASATPVLDGQ
                     ALLSTEGGGARGGLVRGISVEDLRALHPISDNILAGSLADFSSDDAIVVGVGLANTYR
                     LRIGAPLTVISPEGAATAFGTIPRVRAYKVVGIFDAGLSDYNNNVVFLPLAAAQIFFQ
                     KPDAVTGIEIRLHDPDLVSEVGGQLAPLLRGKPVYARDWQHANDTIIGVLQVQKDTMF
                     IVLGMIVLVAAFNVVSSLIMLVKDKRADIAVLRTIGASSFSVLRIFLMCGAFVGVSGT
                     LIGTVIGVLFCHNIAAVQHFVERVTGGRVFDASVFMLTELPNAIDWADVARVVALGLI
                     LSLLATLYPSWRAARTDPVEALRHE"
     gene            complement(2561..>3488)
                     /locus_tag="VGC82_09410"
     CDS             complement(2561..>3488)
                     /locus_tag="VGC82_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY0423524.1"
                     /translation="HIQWKFRDEVRPRFGVMRGREFLMKDAYSFDLDHAGAVTSYRKM
                     MLAYMRTFQRMGLKAIPMEADTGPIGGDLSHEFIILAPTGESQVYYDAAFEEIDYLSS
                     GAGGFSHDKPEDLERFFQGMTTHYAATDEKHDTARWERVSTERKREGRGIEVGHIFYF
                     GTRYTKSMGATVTGPDGKSVHPEMGSYGVGISRLVGAVIEASHDDAGIIWPDSIAPFK
                     AAILNLKIGDASCDAICEQLYAAFEGNALYDDRADRAGAKFADADLMGHPWQIIVGPR
                     GAAAGKVELKRRASGERVEVTPVEALTRCEVR"
BASE COUNT          648 a         1194 c         1061 g          585 t
ORIGIN      
        1 cacgcggatg aggatgcgga tttcggtgtt gcgtcggaca cagaactgca tcacgagtat
       61 tccgggctga caccgacgac cgcgccaggg gcacaaacga tccgcaccgg cgatctggtt
      121 tctctattgg ctgagcgcca accgctcgtc atcgacgtgc tgacatattt ctggggacgc
      181 tccatccccg gcgcggtggg gctgaaacat gccggtgccg gcggcagctt ttcaggcagt
      241 ggtcaggatc ggctgcgcgg taagatggcg gaactgaccg gcggtgacct gtcgaagccg
      301 attgtcgcgg tgggctggaa ttccgaacgc tttgacggac gcaacctggc ccaccgcctc
      361 gtcgccatgg gctacaccaa tgtctactgg taccgcggcg gccgggaaga gtgggaggtc
      421 gccggcctgc ccgaagccga cttggcggca acggactggt agcagcccag cggccctaaa
      481 ccagcatccc ctcccgcagc gtcaccgttc ggtccatccg cgccgccaga tcaggattgt
      541 gcgtggcgat caatgccgcc acaccctgat cccgcacgat cgccagcaat tccgcgaaca
      601 ccgcctcagc cgtggcgaca tccaggttgc ccgtcggttc atccgccagc agcagcttcg
      661 gaccatttgc caaagcacgc ccgatggcca ctcgttgctg ttcgccaccc gacaattttc
      721 ccgggagatg acccagccgc ggcgccaacc cgaacgtcgt cagcagcaac cgagcacgat
      781 cctccgccgc tcgtcgggtt cggccggcaa tcatctgcgg cagcacgacg ttctccagcg
      841 cggtgaattc cggcagcaga tgatggaact gatagacgaa gccgattgta tcccgccgga
      901 ccgcggtgcg ggcggcatcg ggcaatcccc cggcatcgcg gccctcgatc agcaccgacc
      961 caccatcggg ccggtcgagc agccccgcca catgcagcag cgtcgatttt ccggcacccg
     1021 agggcgcgac cagcgccacg atctcgccgg cgcgcaccga caggtcggcg ccgcgcagca
     1081 ccggaaggtc tccggcgtcg ccgcgataga tgcgggtaac ggcgcgcaga accagcggat
     1141 cactcatgcc gcagcgcctc gaccggatcg gtacgggcgg cgcgccaact gggatacagg
     1201 gttgccagca gtgacaggat cagccccagc gccaccacgc gcgccacatc cgcccagtcg
     1261 atcgcgttcg gcagttccgt cagcatgaac accgaggcat cgaacacccg gccgccggtg
     1321 actcgctcca caaagtgctg caccgcggcg atgttgtggc agaacagcac gccgatcacg
     1381 gtcccgatca gcgtaccgct gacgccaacg aaagcgccgc acatcaggaa gatgcgcagc
     1441 accgagaagc tgctggcgcc gattgtccgc aacacggcga tgtcggcgcg cttgtccttc
     1501 accagcatga tcagcgacga caccacgttg aacgccgcga ccagcacgat catgcccagg
     1561 acaatgaaca tcgtatcctt ctgcacctgc aggacgccga tgatggtgtc gttggcatgc
     1621 tgccagtctc gggcatagac cggcttgccc cgcagcagcg gcgccagttg accgccgact
     1681 tcggacacaa ggtcggggtc atgcaacctg atctcgatcc ccgtcaccgc gtcgggcttc
     1741 tggaagaata tttgcgctgc cgccagcggc aggaagacga cgttattgtt gtagtcgctc
     1801 agcccggcat cgaagatccc cacgaccttg tacgcccgca cgcgcgggat ggtgccgaac
     1861 gcggtcgccg ccccctccgg cgagatcacg gtcagcggtg ccccaatccg cagccggtag
     1921 gtgttggcca gtccgacacc gacgacaatg gcatcgtcgc tggaaaaatc ggccaggctg
     1981 ccagccagga tgttgtcgct gatcggatgc agcgcgcgca gatcctcgac gctgatgccg
     2041 cggaccaggc caccgcgcgc ccctccgcct tcggtgctca gcaacgcttg tccatccagc
     2101 accggggtcg ccgaagcaac atccttgatc gcacgaatct ggctcaccag cggctggtaa
     2161 tcgtccagct tctgcccggc ataggcctcg accgagatgt ggccgttcac ccccaggata
     2221 cgcgtcacca gttcggtctg gaagccgctc atgaccgagg tcaccacaat cagcgtcgcc
     2281 acgcccaggg cgatgcccac cagggagaag atggcgatga tcgagacgaa ccgctcgcct
     2341 ttgcgcgcgc gcaggtagcg gccggcgacc atccgctcga acgggccgaa cattcaggcg
     2401 gcctggttca gcggagaaac cgggaccgcg gtgggcaggt ttcggcttaa cgcccggtca
     2461 ctgctgctcg agattatcag gtcatcatcc acggcgacag acccaggttc gcgcagcggg
     2521 gcgaagcagc cgacaacagt gggcctcctc atgtccgata ctatcgaact tcgcagcgag
     2581 tcagcgcttc gaccggcgtc acctcgaccc gctcgccgga tgctcggcgt ttgagctcga
     2641 ccttcccggc cgcggcgcca cgcgggccga cgatgatctg ccacgggtgc cccatcaggt
     2701 cggcatcggc aaacttcgcc cctgcgcggt ccgcgcggtc atcgtacagc gcattgcctt
     2761 cgaacgcggc ataaagctgc tcgcaaatcg cgtcgcagga ggcgtcgccg attttcaggt
     2821 tcaggatcgc cgccttgaac ggagcgatgc tgtccggcca gatgatcccc gcgtcatcat
     2881 gactggcctc gatcaccgcg ccgaccaacc gggatatgcc gacgccgtag ctacccatct
     2941 cgggatgcac cgacttgcca tccggtccgg tgacggtcgc gcccatggat ttggtgtatc
     3001 tggtgccgaa atagaagatg tggccgacct cgatgcccct gccctcgcgc ttgcgctccg
     3061 ttgacacccg ttcccaacga gccgtgtcgt gcttttcatc ggtggcggcg tagtgggtgg
     3121 tcatcccctg gaaaaatcgt tccagatctt ccggcttgtc gtggctgaac ccaccggcac
     3181 cggaagacag gtagtcgatc tcctcgaacg ccgcatcgta gtaaacttgg ctttcgcccg
     3241 tcggcgccag gatgatgaac tcatgactga ggtcaccgcc gatcgggccg gtgtccgcct
     3301 ccatcgggat cgccttcagc cccatgcgct ggaaggtccg catgtaggcc agcatcatct
     3361 tgcggtagct ggtcaccgcg ccggcatgat cgaggtcgaa cgaatacgcg tccttcatca
     3421 ggaattcgcg tccgcgcatc acgccgaagc ggggacgcac ctcgtcgcga aatttccatt
     3481 ggatgtgg
//
LOCUS       DASWQN010000563         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_318438, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000563 DASWQN010000000
VERSION     DASWQN010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318438"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /gene="fliP"
                     /locus_tag="VGC82_09415"
     CDS             complement(<1..658)
                     /gene="fliP"
                     /locus_tag="VGC82_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423339.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEY0423525.1"
                     /translation="MRLPAWVPLALLLLIPAAGFGQSVNIDLGAAGQAGATARLVQIT
                     ALVTVLSLAPSLLVITTAFTRIVIVLSLLRNALSTQGTPPNVVLIGLALFLSFFVMEP
                     AMNAAWTNGIQPMTEGKIAEAEGLRLATEPFHAFMADNVRDDDLRFFMDLGHLPPAQD
                     VKSFPWRALVPAFIIGELRRAFEMGFLLYLPFMVIDLVAGSVLMSLGMMMLPPNAVSL
                     P"
     gene            complement(655..909)
                     /locus_tag="VGC82_09420"
     CDS             complement(655..909)
                     /locus_tag="VGC82_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY0423526.1"
                     /translation="MNAPSAFVVIAVLAGVLALIGAAARVFRLRMWRSQPRAGRTLIL
                     RESIALDPRRRVHLVQCGQRQVVLMTGGGQDLVIGWMQEP"
     gene            1130..1522
                     /gene="flgB"
                     /locus_tag="VGC82_09425"
     CDS             1130..1522
                     /gene="flgB"
                     /locus_tag="VGC82_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEY0423527.1"
                     /translation="MDPTRIGLFDLAEKRLTWTAQRQGVLASNIANANTPGYQARDVQ
                     SFASLLSGHGAVEPAKTQPGHMAGTVPSGLASLLTDPPKARSLDGNTVALDQQLTKVA
                     DTETTQALVTTIWKKYKGMFSMALGRSG"
     gene            1525..1938
                     /gene="flgC"
                     /locus_tag="VGC82_09430"
     CDS             1525..1938
                     /gene="flgC"
                     /locus_tag="VGC82_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEY0423528.1"
                     /translation="MAGDLNQALNISATGMDAQTQRLRVIAQNIANQDTTGSSPGADP
                     YRRKTISFESALDRTAGVETVRVKNVGADGSDLPLRYDPANPASNAQGYVKLPNVNSF
                     IEVMDMREAERSYSANLSVMQATRSMLNKTIDLLK"
     gene            1935..2243
                     /gene="fliE"
                     /locus_tag="VGC82_09435"
     CDS             1935..2243
                     /gene="fliE"
                     /locus_tag="VGC82_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEY0423529.1"
                     /translation="MTTIPSIVVTPSAAADAYNRTARGGASADAANSFGTAITQALDQ
                     AIQSGHTADDQAAKAISGGGNLTSVVAALSHAEMTLQTATAIRDRVVQAYQDIMKMPI
                     "
BASE COUNT          489 a          732 c          686 g          347 t
ORIGIN      
        1 acggcaagct caccgcgttc ggcggaagca tcatcatgcc gagcgacatc agcacgctgc
       61 ccgcaaccag gtcgatgacc atgaacggca gatagagcag gaatcccatc tcgaacgccc
      121 gccgcaactc accgatgatg aacgcgggga cgagcgcccg ccatgggaac gacttgacgt
      181 cctgcgccgg cggcaaatgc cccagatcca tgaagaagcg cagatcgtca tcccggacgt
      241 tgtcggccat gaaggcgtga aatggctccg tcgccagccg caatccctcg gcctcggcga
      301 tcttgccctc cgtcatcggc tggatgccat tggtccaggc ggcattcatt gccggctcca
      361 tgacaaagaa ggacaggaac agggccagcc cgatcaacac gacatttggc ggggttccct
      421 gcgtgctcag ggcgttgcgg agcagcgaca gcacgatgac gatcctggtg aacgccgtcg
      481 tgatcaccag gagacttgga gcgagcgaca gcacggtaac cagggcggta atctgaacaa
      541 gccgagcggt agcgcccgcc tggccagcgg cgccgaggtc gatgttgacc gactgcccga
      601 accccgctgc tgggatcagc aacagcagcg ccagcggaac ccatgccggg agcctcatgg
      661 ctcctgcatc cagccgatga ccaggtcttg cccgccgccg gtcatcagca cgacctggcg
      721 ttgcccgcat tgaacaaggt ggacgcggcg gcgcgggtcc aacgcaatcg actcgcgcag
      781 gatgagcgtc cggcccgccc gaggttgcga gcgccacatc cgaagtctga atacccgggc
      841 tgccgcgccg atcagcgcaa ggaccccggc caggacggca atcaccacga atgctgaagg
      901 agcgttcatc cgcgtgacca ccggggcgaa gccctttcgc ggcggcccgt ggcggcacgg
      961 gttcgaggca accgcccgtc aggaacaggc attgccctgt gcgccatcag cacgaatatc
     1021 gccgcatctg catatgtccg catgcaggca tttgcagcgc cgaaaatgaa taaatcctta
     1081 acggtgccga ttaacttttc atttaccttt ctgcgccaca ttccaaggca tggacccgac
     1141 ccgcattggc ctgttcgatc ttgccgagaa acggctcacc tggaccgccc agcggcaagg
     1201 cgtgctggcg tcgaatatcg ccaatgccaa tacaccaggg tatcaggccc gcgatgtgca
     1261 gtcgttcgcc agcctgttgt ccggtcatgg ggcggtcgag ccggccaaga cgcagcccgg
     1321 ccatatggcc ggaacggttc ccagcggcct ggcgtcgctg ctgacggatc ctccgaaagc
     1381 gcgctcgctc gatggcaaca cggtggcgct cgaccagcaa ttgacgaaag tggcggatac
     1441 cgaaaccacc caggcgctgg tgaccacgat ctggaagaaa tacaaaggga tgttcagcat
     1501 ggctctcgga cggtcgggct gacgatggcg ggtgatctca accaggcgct caacatctcc
     1561 gcgacgggaa tggacgccca gacacagcgt ctgcgcgtga ttgcgcagaa catcgccaac
     1621 caggacacca ccggatcgtc ccccggggcc gacccatatc ggcgcaagac gatctcgttt
     1681 gaaagtgccc tggacaggac agccggggtc gagacggttc gggtcaagaa cgtcggtgcc
     1741 gacggcagcg acctgccgtt gcgctacgat ccggcgaatc cggcttcaaa cgctcagggc
     1801 tacgtcaaac tgccgaacgt caacagcttc atcgaggtca tggacatgcg cgaagccgaa
     1861 cgttcctata gcgccaacct gtcggtcatg caggcaacgc ggagcatgct gaacaaaacg
     1921 atcgacctgc tgaaatgacg accataccga gtattgtcgt aacgccttcg gcggcggccg
     1981 atgcctataa ccgcacggcc cgaggcggcg cttccgccga cgccgcgaac agcttcggca
     2041 ccgcgatcac ccaggcgctc gatcaggcga tccagtccgg acatacggct gacgaccagg
     2101 cggccaaggc gatcagtgga ggcggcaacc ttacgtcggt ggtcgccgcc ctgtcgcatg
     2161 cggaaatgac cctgcaaacc gccaccgcga tccgcgaccg agtggtacaa gcctatcaag
     2221 atatcatgaa gatgccgatc tgagccatga gcga
//
LOCUS       DASWQN010000564         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_977702, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000564 DASWQN010000000
VERSION     DASWQN010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977702"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VGC82_09440"
     CDS             complement(<1..417)
                     /locus_tag="VGC82_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY0423530.1"
                     /translation="MMPHPRIAISILLAGAALLPRAAAAQQSSPGPDAVHIAQAQNIT
                     IGLIAGGPGSTDARIAAEIAQVVDDGDKLRVLPMLGHDSVQNIADLIFLKGVDIAIVH
                     TDVLTQTMQRGMIPREGSVQYIAKLFQEEIHVLARKD"
     gene            581..1543
                     /locus_tag="VGC82_09445"
     CDS             581..1543
                     /locus_tag="VGC82_09445"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0423531.1"
                     /translation="MTDHPLRPDLFRKDAIADDTAQLNDTLVGLLTGLPEWWNVGAEN
                     AREARRQGRGPFPAAIISPRARIMGITGRDGNDIKLRVIAPDRPRGVYMHIHGGGWVL
                     GSSDLQDPMLERIADNTGLAVVSVEYRLAPEDPYPAGPDDCESAAAWLVRNAKAEFGS
                     DVLAIGGESAGGHLAAVTVLRMRDRYGYTGFRGANFVYGAFDLGMTPSQRAFGNRRLV
                     LRTIDMHQFYNAFLPTVADRRVPDISPLYADLKGLCPALFSVGTSDALLDDTLFMHAR
                     WIAAGNEAELAIYPGGAHGFTLFPNRLANEAGARSEAFLRTVTE"
     gene            complement(1639..1995)
                     /locus_tag="VGC82_09450"
     CDS             complement(1639..1995)
                     /locus_tag="VGC82_09450"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY0423532.1"
                     /translation="MPSHERLDCLKCPALCCRMAGYVRVSRDDMRRLAKHLDISVAEF
                     EKRHIVEVTKKGEKRIKEGYKTCQFLDDARMCNVYEARPHDCRGYVCWNQPDETVYGY
                     AVFLQTGVARLRNQEK"
     gene            2095..2295
                     /locus_tag="VGC82_09455"
     CDS             2095..2295
                     /locus_tag="VGC82_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423533.1"
                     /translation="MPLTKIASGGDGLTATALPGGELNGAAASVIDLAASVFALPRAG
                     VPGFTLAVGARPVLGLEGIGLV"
     gene            complement(2335..3387)
                     /locus_tag="VGC82_09460"
     CDS             complement(2335..3387)
                     /locus_tag="VGC82_09460"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEY0423534.1"
                     /translation="MFAYVGGYTTPDRNGRGNGINVYRVDPAIGDWSHIQHIGGLENP
                     ALFTLRRDGSRLYCVHGGRNLLSAFVVDKRSGTLSLLNQMDCQGNNPVDSALDPTERF
                     LVVANYGTGAVAVIPVGEDGRLQPVSQLVQLQGTPGPNPKEQGSSHPHAVIFDPTGQF
                     VVVPDKGFDRTFLFGFQNGRLIPTEQEFVRSAPGAAPRHTTFHPTLPVLYVNNELDST
                     VTVFDWIAGRASERQVVSTLKGGYQRKNTTAEIAVSPCGRRLYVSNRGEDSIVQFVVA
                     PNSGLLSHAGTFPTGGVRPRFFTLGPDAKQLYAANQDSDDITVFNIDKASGGLVPTGV
                     RISVGSPSAISFITSS"
     gene            complement(3389..>3642)
                     /locus_tag="VGC82_09465"
     CDS             complement(3389..>3642)
                     /locus_tag="VGC82_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423535.1"
                     /translation="ACLEEAPFVADTLSMLRAHPVVVIGFFANLGGHVRDDVPALITA
                     ERVARGATGRAVLFHGSVAEDPAMTQIILDQALGDPEGK"
BASE COUNT          674 a         1092 c         1157 g          719 t
ORIGIN      
        1 atcctttcgg gcgagaacgt gtatttcctc ttggaacagc tttgcgatgt actgcaccga
       61 tccttcccgg ggaatcatgc cgcgctgcat ggtctgcgta agaacatcgg tatggacgat
      121 ggcgatgtcg acacctttca ggaagatcag atcggcgatg ttctgcaccg agtcgtgacc
      181 aagcatcggc aggacgcgca atttgtcacc gtcgtccacg acctgggcaa tttcggcggc
      241 gatgcgggca tcggtactgc cgggtccacc cgcgatcagg ccaatcgtta tattttgtgc
      301 ttgagcgata tgaactgcgt ccggccctgg tgagctctgt tgtgccgccg cggccctcgg
      361 caataatgca gcgccggcca acaaaatcga aatggcgatg cgcggatgtg gcatcataat
      421 ctcctcggga taatatgact aaatcgcaac aatataagct cggcccacgg aaaagcgagc
      481 gctttcgctc aagccggacg ctgagctgag atttttgcat tcggcaagat ccggtatagt
      541 gggcaagtgg cttccgtccg tccagatcaa ggagaagtgt atgaccgacc acccattaag
      601 gccggatttg tttcgcaagg acgcgatcgc cgacgacacg gcgcagctga acgacacgtt
      661 ggtcggcctg ctgaccggat tgccggaatg gtggaacgtc ggagccgaaa acgcgcgtga
      721 ggctcggcgg caaggccgcg ggccatttcc agccgcgatc atatcgccgc gggcccggat
      781 catgggaatc accgggcggg acggcaacga catcaagctg cgagtgatcg cgcccgatcg
      841 tccgcgcggg gtttacatgc atatccatgg cggcggctgg gtgctgggat cgtcggatct
      901 gcaagaccca atgctggagc ggatcgccga caacaccggc ctggccgtcg tcagcgtcga
      961 atatcgactg gcgccggaag atccgtatcc ggcgggaccg gacgattgcg aatccgccgc
     1021 cgcctggctg gtgcgcaatg ccaaagccga attcggatcc gacgtgctgg ctatcggcgg
     1081 ggaatcggcg ggcggtcatc tggcggcggt aacggtgctg cggatgcgcg atcgctatgg
     1141 ctacaccggt tttcgcgggg cgaatttcgt gtacggggcg ttcgacctcg gtatgacgcc
     1201 aagccagcgc gcgttcggca accggcggct ggtgttgcgg acgatcgata tgcaccagtt
     1261 ctacaatgcg tttctgccga ccgtcgcgga tcgtcgggtg cccgacatct caccgttgta
     1321 cgccgacctg aaggggttgt gcccggcact gttcagtgtc ggcacgtcgg acgcattgct
     1381 ggacgatacg ctgttcatgc atgcgcgctg gattgccgcc gggaacgagg cggaactggc
     1441 gatctatccc ggcggcgcgc acgggttcac gctgttccca aaccggctgg cgaatgaggc
     1501 aggtgcccgg tcggaggcat tcttgcggac ggtgaccgag tagcccggaa aggccgtaac
     1561 gtccgaagcg cgtagcacca tgtgtggccg gttggcgtgt ggttggtcgg cgtgtggacg
     1621 gccggcgctt ccggatgctt acttttcctg gttcctcaga cgcgcgacgc cggtttgcaa
     1681 aaacaccgca tatccgtaaa ccgtctcgtc cggctggttc cagcagacat agccgcggca
     1741 gtcgtgcggc cgagcctcgt aaacgttgca catgcgagcg tcgtccagaa actggcaggt
     1801 cttgtagcct tccttgatcc gcttctcgcc ctttttggtg acctcgacga tgtgccgctt
     1861 ctcgaattcg gcgacgctga tgtcgagatg cttcgccagg cgcctcatgt cgtcgcggct
     1921 gacccgtaca tagcctgcca tgcggcagca cagtgccgga catttcaggc aatccagacg
     1981 ctcatgcgat ggcatcgctg atgtgtggga ctggccgaag gaagctgcaa ggcctcaagc
     2041 gagttccacg gtcttgaagt ccgcggggac gatgatctcc cgggcgttga aaccttgccg
     2101 ctgaccaaga ttgcctctgg tggcgatggc ttgaccgcga cggccttgcc gggcggcgaa
     2161 ctgaacggag cagcggcctc cgtcatcgat ctggccgctt ccgtcttcgc tttgccgcgt
     2221 gcgggtgtac ccggtttcac gttggcagtt ggtgcccgcc cggtgctggg tttggaaggc
     2281 atcggcctcg tgtaatttcg aacgcaacaa tatcgccgcg gtcggcccga tctttcaaga
     2341 cgatgttatg aagctgatcg ccgagggact gcccacgctg atccgcacgc cggtcgggac
     2401 caggcccccg gaagccttgt cgatattgaa aacagtgatg tcgtcactgt cctggttggc
     2461 cgcgtaaagc tgctttgcgt caggcccaag ggtgaagaac cgaggcctga cgccgccggt
     2521 ggggaacgtg ccggcgtgtg atagcaatcc gctgttcgga gcgacaacga actggacgat
     2581 gctgtcctcg cctcggttcg aaacatagag acggcgtccg caaggcgaca ccgcgatctc
     2641 cgccgtcgtg ttctttctct gatagccgcc cttcagcgtc gagacgacct gccgctcgct
     2701 tgctcggccg gcgatccagt cgaataccgt caccgtcgaa tcgagctcgt tgttcacata
     2761 cagcaccggc agcgtgggat ggaacgtggt atgccgaggc gccgcacccg gtgccgaccg
     2821 gacaaattcc tgttccgtcg ggatcagccg cccgttctgg aagccgaaga ggaaggtgcg
     2881 gtcgaagccc ttgtccggga cgaccacgaa ctgtccggta ggatcgaaga tcaccgcatg
     2941 aggatgtgac gacccctgct ccttcggatt ggggcccggc gttccctgta gctgaaccaa
     3001 ctggctgacc ggttgcagtc gcccatcctc acccaccggg atcacggcca cggcgccggt
     3061 tccgtagttg gcgacgacca gaaaacgttc cgtcggatca agggcggaat caaccggatt
     3121 gttgccctgg caatccatct ggttgagcaa gctcagcgtg ccggagcgtt tgtcgacgac
     3181 aaaggcgctc aggaggttgc ggccgccatg cacgcagtaa agccggctgc catcgcgcct
     3241 cagggtgaac agcgctgggt tttccagccc gccaatgtgc tggatgtggc tccagtcgcc
     3301 aattgccgga tcgacgcgat aaacgttgat cccgtttccc cgcccgttcc gatcgggtgt
     3361 ggtgtagccg ccgacgtaag caaacatgtc atttcccctc gggatcgcca agcgcctgat
     3421 ccaggatgat ctgcgtcatc gccggatcct cggctacaga accatgaaac agcaccgctc
     3481 gcccagtagc tccgcgggct acccgttcgg ctgtaattag cgccggaacg tcatcgcgga
     3541 catggccgcc caaattggcg aagaagccga tcaccaccac ggggtgtgcg cgcagcatgc
     3601 tcagcgtgtc ggcgacgaag ggtgcttcct ccaggcacgc cg
//
LOCUS       DASWQN010000565         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2033581, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000565 DASWQN010000000
VERSION     DASWQN010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033581"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VGC82_09470"
     CDS             complement(<1..831)
                     /locus_tag="VGC82_09470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423536.1"
                     /translation="MRLRFKATHVVLAMLCVMYFITYIDRVNIGTAASEIQKELGLSN
                     TQLGLVFSAFAYPYLLFQVIGGWVGDRFGPRRTLFWCGMIWAVATIMTGFVHGLLTLF
                     IARVALGFGEGATFPTATRAMQYWTPAAKRGFAQGLTHAFARLGNAITPPLIAALMAW
                     LTWRGSFIALGLVSLVWGVVWAWYFRNEPKDHPGITEAELASLPPRPANMRPQVPWGP
                     LLRRMWPVTLTYFCYGWCLWLYLNWLPLFFKNSYHMDIKNSALFASGVFFAGVVGDSL
                     G"
     gene            complement(902..2110)
                     /locus_tag="VGC82_09475"
     CDS             complement(902..2110)
                     /locus_tag="VGC82_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEY0423537.1"
                     /translation="MTVHTGRHFLQIPGPTNVPDRVLRAMDMPTMDHRGAEFAEIGYA
                     VLAAMQRVFRTKQPVIIYPSSGTGAWEAAIVNTLSPGDKVLMVETGHFATLWQQMAAR
                     WGIEVDFMPGDWRHGADPAAIEARLAQDSSHSIKAVMVVHNETSTGVTSRVGDIRAAM
                     DKVGHPALLLVDTISSLGSVDYRHDEWKVDVTVSCSQKGFMLPPGLGFNAISEKARAA
                     AKTNKMPRSYWDWEEMLKPNANGFFPYTPATNLLYGLREAIAMLLEEGLDAVFARHQR
                     LAAATRAAVRHWGLEVLCLEPSEYSPVLTAVLMPPGHDADQFRKTVLDNYNMSLGSGL
                     TKLAGKVFRIGHLGECNELTLLGALTGVEMGLSLAGVPHRAGGVAAAMTFLEERTQSN
                     TSAHLKVISN"
     gene            complement(2355..>3119)
                     /locus_tag="VGC82_09480"
     CDS             complement(2355..>3119)
                     /locus_tag="VGC82_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY0423538.1"
                     /translation="RYWATLLRSSIIGAWLGITPGGAIAASFMGYNVAKRFAKDKESF
                     GKGRIEGVFAPETAAHASGTAALLPMLALGIPGSGTAAILLGGLMVWGLNPGPLLFVE
                     HKDFVWGLIASMYLGNVVGLALVLTTVPIFASILRVPFAAVAPMIVVSCAIGAYAIQN
                     AMFDIWLMLGFGVVGYVFKKIGIPLAPFTLALVLGNRAEDAFRLSMIGSGGAMKVFWS
                     NGLVGSITTLAIVLLFWPLIEKILGGFGRFWRPASI"
BASE COUNT          649 a          997 c          959 g          514 t
ORIGIN      
        1 gccaaggctg tcgccgacga ccccggcgaa gaaaacgccc gaggcgaaca gcgccgaatt
       61 cttgatgtcc atatggtagc tattcttgaa gaacagcggc agccagttga gatacagcca
      121 caaacaccaa ccgtagcaaa agtacgtgag tgtgactggc cacatccggc gcagcagcgg
      181 cccccatggc acctgcggcc tcatattggc aggacgcggc ggaagcgacg ccagttcggc
      241 ctcggtgatg ccaggatgat ctttgggctc gttgcggaaa taccaagccc agaccacgcc
      301 ccagaccagg cttaccagtc caagcgcgat gaacgaaccg cgccaggtca gccaagccat
      361 cagcgcggcg atcaacggcg gcgtgattgc gttgccgagt cgcgcgaagg cgtgggtgag
      421 gccctgcgcg aaaccgcgct tggccgcggg ggtccagtat tgcatagccc gcgtggcggt
      481 tgggaaggtc gctccttcgc cgaacccaag ggcgacacga gcgataaaca acgtcaggag
      541 cccgtgcaca aagccggtca tgatggtggc aaccgcccag atcatgccgc accaaaacag
      601 cgttcggcgc ggtccgaaac ggtcaccgac ccaaccgccg atgacctgga acagcaaata
      661 gggataggca aaggctgaaa acaccaggcc aagctgcgta ttgctgagac ccaattcctt
      721 ttgaatttcg ctggccgcag tgccgatatt tacccgatcg atataggtga tgaagtacat
      781 gacgcacagc atcgccagca cgacatgggt ggccttgaaa cgaagccgca tgttctaact
      841 cccggacagt ccgcctatgg ttcggatctg cttgtgaacc gaacccgcgt gaccaccttg
      901 ctcagttcga tatcaccttg agatgcgccg acgtgttgga ctgggtgcgc tcctcaagga
      961 acgtcatcgc cgccgccacg ccaccggcgc gatgcggtac gccggcgagg gacaatccca
     1021 tttcgacacc ggtcagcgcg cctaacagcg tgagttcgtt gcactcaccg agatgaccga
     1081 tgcgaaacac tttgccggcg agcttcgtca gcccggaacc gagcgacatg ttgtaattgt
     1141 cgaggacagt tttgcggaat tggtcggcgt cgtgtccggg tggcatcagc acggccgtca
     1201 gaaccgggga atactccgaa ggctccagac acagcacttc gaggccccaa tgccgcaccg
     1261 ccgcgcgggt ggcagccgcg aggcgctgat gacgggcgaa taccgcatcc agcccttcct
     1321 caagcagcat cgcgatggct tcgcgaagtc cgtaaagcag attggtggca ggcgtgtaag
     1381 gaaagaagcc gtttgcattg ggcttcagca tttcctccca gtcccaataa gaccggggca
     1441 tcttgttggt cttggccgcg gcgcgggcct tctccgagat cgcattgaag ccgagccccg
     1501 gcggcagcat gaagcccttt tgcgaacagc tgacggtgac gtcgaccttc cactcgtcat
     1561 gccggtaatc gaccgagccc agcgaggaga tcgtatcgac aagcagcagc gcgggatggc
     1621 cgaccttgtc catcgcggcg cggatgtcgc cgacgcgact ggtcacgcca gtggaagttt
     1681 cgttgtgcac caccatgacc gccttgatcg aatgcgacga atcctgcgcc agcctggcct
     1741 cgattgccgc gggatcagcg ccgtggcgcc agtcgccggg catgaagtcc acctcgatgc
     1801 cccaacgcgc tgccatctgc tgccacaggg tggcgaaatg gccggtctcg accatcaata
     1861 ccttgtcgcc cggcgagagc gtgttgacga tcgcggcctc ccaggcgccg gttcccgacg
     1921 agggatagat gatgacgggc tgcttggtgc gaaacacccg ctgcatcgcc gccagcaccg
     1981 cgtagccgat ctcggcgaat tccgcgccgc gatggtccat ggtgggcatg tccatggcgc
     2041 gcagcacccg gtccggaacg ttggttggtc ccggaatctg cagaaaatgc cttccagtgt
     2101 gcacggtcat gtcggcgtcc ttcccggggg tagctgtcgt ttggcagcgc tcgaatatca
     2161 ctatttggct gccttgtccc ctgcccggcg ggacccgtca atgcataaca agcagggctg
     2221 cgcccgccct ggccggctcc cgggatacta cgaagccgga tcccggagac accggaaaat
     2281 cgcgtgaatt tggccagccg cgaccgctaa cggcgacctc ggcatgggtg aatatccggg
     2341 cgccccacgg ctattcaaat gctggctggc cgccagaatc ggccaaagcc gccgaggatt
     2401 ttttcgatca atggccagaa cagcagcacg atcgccagcg tcgtgatcga accgaccagg
     2461 ccgttcgacc agaacacctt catggcgccg ccggaaccga tcatcgacag ccggaatgcg
     2521 tcctcggcgc gattgcccag caccagcgcc agcgtgaacg gcgccagggg aatgccgatt
     2581 ttcttgaaga catagccgac cacgccgaat cccagcatca gccagatgtc gaacatcgcg
     2641 ttctggatcg cataggcgcc gatcgcgcag gataccacga tcatcggcgc cacggcggcg
     2701 aacggcaccc gcaggatgga ggcgaagatc ggcaccgtgg tcagcaccag cgcgagaccc
     2761 accacgttgc cgagatacat cgatgcgatc aggccccaga caaaatcctt gtgctcgaca
     2821 aacagcagcg gcccggggtt gaggccccac accatcagcc cgccgagcag gatcgccgcg
     2881 gtgcccgagc ctggaatgcc cagcgccagc atcggcagca gtgctgcggt gcccgaggca
     2941 tgtgcggcgg tctccggcgc gaatacgccc tcgatgcggc ccttgccgaa ggattccttg
     3001 tccttggcga agcgcttggc gacgttgtag cccatgaacg aggcggcgat ggcgccaccc
     3061 ggcgtgatgc ccagccaggc gccgataatc gaggagcgca gcagcgtcgc ccagtagcg
//
LOCUS       DASWQN010000566         3234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_846475, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000566 DASWQN010000000
VERSION     DASWQN010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846475"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VGC82_09485"
     CDS             <1..571
                     /locus_tag="VGC82_09485"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0423539.1"
                     /translation="PPGAVVSEAILSRRLGVGRTPIREALQRLAREWLVVIMPRRGIV
                     VSEIDTVRQLRVLEIRREIERFLAQSAAKRATPVQRAQFQEIATGMDIAAEQADDLTF
                     MRLDREFNLLLLDAAGNEFAASAMGQMNGLSRRFWYSHYKQVADLPLAARLHATIARA
                     LAQGNQEAAAQSSDALIDYIETFARATIG"
     gene            complement(602..2686)
                     /locus_tag="VGC82_09490"
     CDS             complement(602..2686)
                     /locus_tag="VGC82_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY0423540.1"
                     /translation="MRPASLAPDLARLPIVVNARAISLAEGVRAGLSVAVIISLNEYL
                     SFAPLREAALAALLTCICDPGGPIRRRVPVLLSFALTGATISASYGLLREFGPVVALP
                     LGVFGLFCSSFARIYGQAPQQLGALLATVQILALDRGNPSLTSALVLAGSFIGGALWA
                     TLLTMVIWRIYPFLPARRAVAEAYRRLSILVADLRSLVQAPWITDAAWEAHARIHRRA
                     TREAMEAARTAVMDAARSRGAAGNRAAQAIIRLETADQIFGGLIALSDLLEHGSVAER
                     AVAERVLRRMRPVLLVLGRVVVTDDPDAHRRIDRAIGSITGELTVLAADSPLRSVMER
                     ITERLQIARTLAVPANYSPGVSLEGRPLPLAQRLMRPLRANLNWSSPALRHALRSAVT
                     AAPALAFTMFWFSPYDHWLTITIVATMQPYFSLAYTRAIERIIGTAAGGLIAAAAGLV
                     CTTPFSIAGAMFILAIAAFTVRSVSFGLFMMALTPLVVLLVETGAPDTGDWQIAFVRA
                     AFTTIGGLIAVSANFLLWPSREPDLVAPEVKKAIAAHATYVEAGFSSLLEETSRTPLV
                     SARREAGVASNALEALISRALLEPGNKKHDQLEAAMVIDAALRRCAGRLATLPHDPTI
                     MQGVPKLTLRTWGDWIVESLRLLAAGKTELSPRPPGNDSDALARMARQIELMSGAIPR
                     LEQIAGGPNGRK"
     gene            complement(2693..3142)
                     /locus_tag="VGC82_09495"
     CDS             complement(2693..3142)
                     /locus_tag="VGC82_09495"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY0423541.1"
                     /translation="MTRHAMQAAMDEARTAGARGEIPVGAVVTGPDGTILTRAGNQTE
                     ADNDPTAHAELLALRAAAALRASPRLPDCDLVVTLEPCPMCAQAISLFRIRRLIFGAY
                     DPKGGGVDHGARIFEASSCHHRPEVVGGVRETEAATLMRDFFREKRQ"
BASE COUNT          595 a          987 c         1132 g          520 t
ORIGIN      
        1 ccccccgggc gccgtcgtct ccgaagcaat cctcagccgc cggctggggg tagggcgcac
       61 acccatccgc gaagcgctgc aacgactggc gcgcgaatgg ttggtcgtca tcatgcccag
      121 gcgagggatc gtggtatcgg aaatcgacac ggtccgccaa ctgcgtgttc tggagatccg
      181 gcgggagatc gagcggtttt tggcccaatc cgccgccaaa cgagctacgc cggtccagcg
      241 cgcccagttc caggagatcg cgaccggcat ggacatcgca gcggaacagg ccgacgatct
      301 cacattcatg cggctggatc gggaattcaa cctgctgctt ctggacgctg ccggcaacga
      361 gttcgccgct tccgccatgg gtcagatgaa cggattgtcc cgccggttct ggtacagcca
      421 ctataagcag gtcgcggacc tgccattggc ggcacgtctg catgcaacca tcgcccgcgc
      481 cctcgcgcag ggcaaccagg aggcggcggc gcaaagctcg gatgcgctta tcgactacat
      541 cgagacattt gcgcgggcga cgatcggcta agcactttag tccgttgatt cgtaataatt
      601 actacttccg accgttcggc cctccggcga tctgctctaa tcgcggtatt gccccggaca
      661 tcagttcgat ctggcgggcc atccgagcaa gcgcatcgga atcattgccg ggtgggcgcg
      721 gggacagttc cgtcttgccg gccgccagca ggcgcagcga ttcgacgatc cagtcgcccc
      781 aggtgcgcaa cgtaagcttc ggcacgcctt gcatgatcgt cgggtcgtgc ggcagcgtgg
      841 cgaggcgtcc cgcgcaccgg cgcaacgcgg cgtcgatgac catcgctgcc tctaattggt
      901 cgtgcttctt gttgccaggc tccagcagcg ctcggctgat caatgcctcc aatgcattac
      961 ttgcgacgcc ggcctctcgt cgcgcgctga caagaggggt gcgtgatgtt tcttcgagca
     1021 acgaagaaaa accggcctcg acgtaagtag catgggcggc gatggccttc tttacctcgg
     1081 gtgccaccaa atctggctcc ctgcttggcc acagcaggaa gttggcgctg acggcgatca
     1141 ggccaccaat cgtggtaaag gcggcgcgga cgaaggcaat ctgccagtct ccggtgtcgg
     1201 gcgccccagt ttccaccagc aataccacga gcggcgtaag agccatcatg aacaggccga
     1261 agctgaccga gcggacggtg aaggcagcaa tggcgagaat gaacatggcg ccggcgatgc
     1321 tgaagggcgt tgtgcacaca agcccggccg cggcggcgat caggccgccc gctgcggtgc
     1381 cgatgatgcg ttcaatcgcc cgggtgtagg ccaaagagaa atacggctgc atcgtcgcca
     1441 cgatcgtgat cgtcagccag tggtcgtagg gactgaacca gaacatcgtg aaagcgagag
     1501 cgggcgcggc ggtgacggcg gagcgcaacg cgtggcgcag ggcaggggag ctccagttga
     1561 ggttggcgcg caacggacgc atcaaccgct gcgccaaagg aagcgggcgt ccctcgaggc
     1621 tgacgccggg ggaatagttc gccggcaccg cgagggtgcg ggcgatttgc agccgttcgg
     1681 tgatacgttc catcacgctg cgcaagggcg agtctgccgc cagaactgtg agttcgccgg
     1741 tgatcgaccc aatcgcccga tcgatgcgcc gatgcgcatc ggggtcgtcg gtcacgacaa
     1801 cgcggccgag aaccagcagc accggacgca tccgccgcag tacgcgttcc gcaacggctc
     1861 gttcggcgac cgagccgtgc tccaacaagt cgctcagggc gatcaggccg ccaaagatct
     1921 ggtccgcggt ttccagccgg atgatggcct gggcggcgcg gttgccggcg gcaccgcggg
     1981 agcgggcggc gtccatgacg gcggttcgag cggcctccat cgcttcccgt gtcgcgcggc
     2041 gatggatgcg ggcgtgggct tcccacgccg cgtccgtgat ccatggcgcc tgcaccaggc
     2101 tgcgcagatc ggcaaccagg atcgatagtc ttcggtaggc ttcggccacg gcccgccggg
     2161 cgggaaggaa cggatagatg cgccagatga ccatggtcag aagcgttgcc cacagggcgc
     2221 cgccgatgaa cgaaccagcg agcacgagag cgctcgtaag gctgggatta ccgcgatcga
     2281 gcgccaggat ctgcaccgtg gcgagcagcg ccccgagttg ctgtggggcc tggccataga
     2341 tgcgggcaaa ggaggagcag aacaggccga aaacaccgag cggcaacgcg acgacggggc
     2401 cgaattcccg tagcaggccg tagctggcgg atatggttgc gccggtcagg gcaaaactca
     2461 gcagcacggg aacacggcgg cggatggggc cgccggggtc gcagatgcag gtaagcaacg
     2521 ccgcgagcgc ggcctcgcgc aacggtgcga acgacagata ttcgttcaag gagatgatga
     2581 cggcgactga caggccggcg cgcacgccct cggccaggct gatcgctcgc gcgttgacga
     2641 cgatcggcag gcgcgcaagg tcaggggcta gggaggcggg tcgcatgacg gcttattgcc
     2701 gcttctcccg gaaaaagtcg cgcatcaagg tcgcggcctc ggtttcgcgc acgccaccca
     2761 cgacctctgg ccggtgatgg caggacgagg cttcgaaaat gcgggcgccg tgatcgacgc
     2821 cgccgccttt gggatcgtag gcaccgaaga tcaggcggcg gatgcgaaac aggctgatgg
     2881 cctgcgcgca catcgggcag ggctccagcg tcacgacaag gtcgcaatcg ggcaggcgag
     2941 gactggcgcg cagggcggct gcggcgcgca gggcaaggag ttcggcgtgg gcggtggggt
     3001 cgttgtcggc ctccgtctgg ttgccggcgc gggtgaggat tgttccgtcg ggaccggtga
     3061 ccacggcacc gacggggatt tcaccccggg cgccggcggt gcgggcttca tccatcgcgg
     3121 cttgcatggc gtgtctggtc atgagccgtt atagtctgtt gcttgcgcgg caggggaact
     3181 gtatagcgtg agccggtgtt tgccggcgta gctcaggggt agagcaactg attc
//
LOCUS       DASWQN010000567         1951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2562000, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000567 DASWQN010000000
VERSION     DASWQN010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2562000"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VGC82_09500"
     CDS             <1..313
                     /locus_tag="VGC82_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423542.1"
                     /translation="GNGEEASGDGWRFRGHGLIQITGRDAYRRFAQAMAMTLDQAVEH
                     AGTRTGAADSAVWFWTANQLNALATSWSVDLLTRKINGGSAGAAERNRLCEAALHAIG
                     A"
     gene            315..953
                     /locus_tag="VGC82_09505"
     CDS             315..953
                     /locus_tag="VGC82_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423543.1"
                     /translation="MFAALIPLVLSLAPQLAGMIFGAKGADATVKVAGIVQAVVGTAA
                     DLSTPGGAAAAVAHFQANEQASSELAAKLADLHLQMQKEQDAEADAFRKDTLDDLKAR
                     LDDTGSARNMATELAKVNSGLAYGGVVVSVVIVGAFGCTTYSVLNNHMSAADGQFGSI
                     LVGTLAAMATQVANYWLGSSIGSNTKNALLANAQNNLASSVPGRMVKSDARI"
     gene            complement(1000..>1951)
                     /locus_tag="VGC82_09510"
     CDS             complement(1000..>1951)
                     /locus_tag="VGC82_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY0423544.1"
                     /translation="SACEAAIRLHPDLAQAHWNMAIAALLSGNLRRGFSEYEWRKQHA
                     RYRADLPALPGRPWDGTDPSDQTVLVRAEQGFGDAIQFARYLPEIRAAGGIPVMLCAP
                     PLVPLFQSMLGVAAVSTGEPMPAYDLWIDQASLPGIFGTLLDTIPAADRYLRADPIRT
                     EAWRGRLPRGRKVGVAFAGNPKHPGDRRRSIPVHLMDMLPAIPDLAYVSLQHGNLAKG
                     LGLPDQTPWMTDYAETAALIENLDLVITADTSVAHLSGALGKPVWILLPHAPDWRWLL
                     HRSDSPWYQSARLFRQPTAGDWVSVVAQAARALENHWLFV"
BASE COUNT          381 a          621 c          640 g          309 t
ORIGIN      
        1 gggcaacggc gaggaagcat ccggcgacgg ctggcggttt cgcggccatg gcctgatcca
       61 gatcaccggc cgcgatgcgt atcggcgctt cgcgcaggcg atggccatga ccctggatca
      121 ggccgtggaa cacgccggca cgcgaaccgg ggccgccgac agcgccgtct ggttctggac
      181 ggcaaaccaa ctgaatgcgc tggccacgtc gtggtcggtc gatcttctta cccgaaaaat
      241 caacggcggt tcggctggcg cggcggaacg aaaccgcctt tgcgaggccg cactgcacgc
      301 aattggagct tagcatgttc gcagccttga tccccctggt gctcagcctg gcgccgcaac
      361 tcgccggaat gatcttcggc gcgaagggcg ccgacgcgac cgtcaaagtg gcgggaatcg
      421 tccaggcggt tgtcgggacc gccgccgatc tcagcacgcc gggcggcgcc gccgcggcgg
      481 tcgctcattt ccaggccaat gaacaagcat cctccgaact ggctgcaaaa ttggcggatc
      541 tacacttgca gatgcagaag gaacaggatg ccgaggccga tgcgtttcgc aaggacacct
      601 tggatgacct gaaggcccgc ctggacgaca cgggttccgc ccgaaacatg gcgacggaac
      661 ttgcgaaggt gaacagcggc ctggcatacg gcggcgtggt cgtttcggtg gtgatcgtcg
      721 gagcctttgg ctgcacgacc tattcagtgc tgaacaacca catgagcgcg gcggatgggc
      781 agttcggctc gatcctggtt ggaacactgg cggcgatggc aacccaggtc gcgaactact
      841 ggctgggctc cagcatcggc agcaacacca agaacgccct gctggcaaac gcgcagaaca
      901 atctcgcgtc gagcgtgccg ggccgcatgg taaagtcaga cgcgcggatc tagcgcgtga
      961 acacggtggg tggaacgtcc gaagcctgta atcatgcgat caaacaaaaa gccagtgatt
     1021 ttccagcgcc cgcgccgctt gggcgacgac gctaacccag tctccggcgg tcggttgacg
     1081 aaacagacgc gccgattgat accacggact gtcgctacgg tgaagtaacc agcgccagtc
     1141 cggagcatgc ggcagcagga tccataccgg cttgcccaag gcgccggaga ggtgggcgac
     1201 tgacgtatcg gccgtgatca ccagatccag attctcaatc aacgcggcgg tttcggcata
     1261 gtccgtcatc cacggggttt ggtcaggcaa ccccagacct ttggccagat taccatgctg
     1321 cagactgacg taagccagat ccgggatcgc cggcagcatg tccatgagat gcactggtat
     1381 cgaacgccgg cggtctcccg ggtgcttcgg gttgccagcg aacgccaccc cgaccttccg
     1441 cccgcgaggc aaccgcccgc gccatgcctc tgtgcggatc ggatcagccc gaaggtagcg
     1501 gtccgccgcg ggaatggtgt ccagcaatgt cccgaaaata cccggcagac tggcctgatc
     1561 gatccagagg tcatacgccg gcataggctc cccggtggag actgccgcga cccccagcat
     1621 cgactggaaa aggggaacca gcggcggcgc gcacagcatg acgggtatgc ctcccgccgc
     1681 gcggatctcc ggcaggtacc gggcgaactg aatcgcgtcg ccgaagcctt gttcggcacg
     1741 caccagaaca gtctgatccg acggatcggt gccgtcccac ggccggccgg gaagcgcggg
     1801 gagatccgcc cggtaccgag cgtgctgctt gcgccattca tactcgctga aaccgcgccg
     1861 caggttgccg ctcagcaggg cggcgatcgc catgttccag tgcgcctggg cgaggtccgg
     1921 gtgcaggcgg atcgccgcct cgcaggcgct g
//
LOCUS       DASWQN010000568         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_713624, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000568 DASWQN010000000
VERSION     DASWQN010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713624"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VGC82_09515"
     CDS             <1..257
                     /locus_tag="VGC82_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423545.1"
                     /translation="LAGFAGRGVLCVGAPADIVIYDYDNLEVLPSEIVHDLPGGEWRR
                     VQHARGYRYVLVNGGITIEDDRETNTFTGRLLRGGGRVAP"
     gene            327..>1547
                     /locus_tag="VGC82_09520"
     CDS             327..>1547
                     /locus_tag="VGC82_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0423546.1"
                     /translation="MLRARVHQHPDRIAVVDGSRQFSFAAFNARVNQLTVLLRTLDIA
                     RGDRVAILSENRVEYLELMFAAGKLGAIVCALNWRLADPELLHCVTLTSPKATFVSLA
                     YGGVARRLSNVLGDTVLLGEDYEQRLAHASAEEPEIAAEAEDGHVILYTSGTTGNPKG
                     ALISHRAQLARMHSSCVDFELAPGDTFVAWAPMFHMASTDQSIHVLCLGGKVIIIPGF
                     DVDRILHAAETETLWWLLVMPGMIEDMIAGAKRRRTELRGIKLLGAMADLVPRHQLAE
                     ITSLLQAPYANTFGSTETGLPPASAGRLAIGTAPSCLSKRPSASCILRLVDAEDRDVP
                     DGLPGELLIRGPTLFSGYWNEPHATAHDFRGGWFHMGDVFTRNPDGTISFIDRVKYLI
                     KSGGENIYPAEIEQV"
BASE COUNT          282 a          455 c          481 g          329 t
ORIGIN      
        1 cgcttgccgg ctttgcgggc cgaggcgtgc tgtgcgtcgg cgcgcctgcg gatattgtca
       61 tttatgacta cgataatctc gaggtactgc cgagcgagat cgtccacgat ctgcccggcg
      121 gcgaatggcg gcgtgtgcag catgcgcgtg gctatcgtta tgtgctcgtg aacggtggca
      181 taacgatcga agacgatcgc gagaccaaca cttttacggg tcgcctactc cgcggtggcg
      241 gtcgggtggc gccttaggcg gccagaatgc ttaattgcat agatcctacg ctcgcgcgcc
      301 atgcgtcagg tatcacgatc ggctcgatgc tgcgggcccg cgtgcaccag catcccgacc
      361 ggatcgcggt tgtcgatggc agccgccagt tcagcttcgc cgcgttcaac gcccgggtga
      421 accaactgac cgttctgctc cggacgcttg atatcgcacg tggggatcgg gttgcgattt
      481 tatcggaaaa tcgcgttgaa tacctcgaac taatgttcgc cgccggtaag ctcggtgcca
      541 tcgtatgcgc gcttaactgg cggctcgccg atcccgaact gctgcactgc gtgacgttga
      601 cctcgccaaa agcgacgttc gtctcgcttg cctatggggg cgttgccagg cgattgtcga
      661 acgtcttggg cgacactgtc ctgctgggcg aggactacga acagcgcctc gcccacgcga
      721 gcgccgagga acccgaaatc gcggccgagg cggaggacgg gcatgtcatc ctctacacca
      781 gcggcacgac cggcaacccc aagggtgcgt tgattagcca tcgtgcgcag ctggcgcgaa
      841 tgcattcgag ctgcgtggat ttcgaactcg cacctggcga cacgtttgtt gcttgggcac
      901 cgatgttcca catggcctct accgatcagt cgatccatgt gctgtgtctt ggcggcaagg
      961 tgatcatcat tcccggtttt gacgtcgatc gtatcctcca tgcggcagaa accgagacgc
     1021 tgtggtggct tctcgtgatg cccgggatga tcgaggacat gatcgcaggc gcgaaacgcc
     1081 gtcgcaccga actgcgcggg atcaagctgt tgggtgctat ggccgacctt gtgccgcgcc
     1141 atcaactggc agagatcaca agcctgctgc aggcgcctta tgccaacacg tttggttcga
     1201 cggagacagg tctgccacct gcgtcagccg ggcgcctggc gattggcacc gctccttcct
     1261 gtctgtccaa acgaccaagt gcgagttgca tattacgtct ggttgatgcg gaggatcgtg
     1321 atgtgccgga tggcctgcca ggggaacttt tgatccgggg acctacgctg ttcagcggtt
     1381 actggaacga gccacacgcg accgcgcatg attttcgcgg cggctggttc cacatgggtg
     1441 acgtcttcac ccgcaacccc gatggcacga tcagctttat cgaccgggtg aaatacttga
     1501 tcaagtccgg cggcgagaac atttatccag ctgagatcga acaggtc
//
LOCUS       DASWQN010000569         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1374652, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000569 DASWQN010000000
VERSION     DASWQN010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374652"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1742)
                     /locus_tag="VGC82_09525"
     CDS             complement(123..1742)
                     /locus_tag="VGC82_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0423547.1"
                     /translation="MQQETYDFIVTGAGSAGAVVASRLSESGRHRVLLLEAGPPDTNP
                     WIHIPLGFAKTFIDPAVNWKFESAPQPALGNRRLYLPRGKTLGGSSSINGMVYIRGNH
                     ADYDEWRQRGCTGWDWDSVLPFFRKAQNQVRGASEYHGAGGPLNVSDQPKRFELSDAV
                     LAACEQAGLPRNPDFNGAVQEGCGYYQTTTSNRRRWTTAKAYLDPARSRANLTIRTGA
                     HATRVLIADGRASGVEYVCTEGRCVALAGREVIVSGGSYGSPQLLQLSGLGPAQHLQD
                     MGVPVVRDMPAVGSNLHDHFNTYMSWRCTRPITLNDLENSWPRKIAAAVRYGLFRSGP
                     MASNGIHAGLFTRSDPRLERPDLQINLLEWSTLERTKDRVVPHRFPGFTLSPVHLKPD
                     GRGSVRLASPDPLTPPTILFDFLRTDYDMRAVVTGIRLARSIASQPALRPYVAEEIMP
                     GPAVTSDADMEAFVRQTGVSNHHPSSTCAMGTGTNSVVDARLRVHGMDGLRVADASIM
                     PSVVAGNTNAPSIMIGEKAAAMILEDARLAA"
     gene            1837..>2071
                     /locus_tag="VGC82_09530"
     CDS             1837..>2071
                     /locus_tag="VGC82_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY0423548.1"
                     /translation="MLEVDHPADMKAYIGKPVGSSEWALVDQTMIDAFANATGDHQWI
                     HVDVDRAKKEMPNGTTIAHGYLTLSLLPRLGASI"
BASE COUNT          372 a          645 c          684 g          370 t
ORIGIN      
        1 gttcttgtcc ggcggcccgg cttccagcag cagtacgcgg tagcggccgg attccgctaa
       61 tcggccggca atagcgcagc ccgccgatcc agcaccggtg acgatgaagt cgaaggcatc
      121 gttcatgccg ccagccttgc gtcttccagg atcatcgcag ccgctttttc cccgatcatg
      181 atgctcggtg cgttggtatt gcctgcgacg acggacggca tgatcgaggc atccgccacg
      241 cgtaggccgt ccatgccatg cacgcgcaat cgggcatcga cgaccgaatt ggtgccggtt
      301 cccattgcgc aggtgctgct tggatgatgg ttggaaacac ctgtctgacg cacgaacgcc
      361 tccatgtccg cgtccgacgt cacggcgggg ccgggcatga tctcctccgc tacataaggt
      421 cgcagggcgg gttgactggc gatcgacctc gccagccgta ttcccgtcac caccgcacgc
      481 atgtcgtaat cggtgcgcag aaaatcgaac aggatcgtcg gcggcgtcag cggatcggga
      541 ctggccagcc gcaccgaacc acggccatcg ggtttcagat gcacggggct cagggtaaaa
      601 ccggggaagc ggtgcgggac gacgcggtcc ttggtgcgtt ccagcgtgct ccattccagc
      661 aggttgatct gcagatccgg ccgttccagg cggggatcgg accgggtgaa caaccccgca
      721 tgaatgccgt tgctggccat cgggccggag cggaacaggc cgtaacggac ggcggcggcg
      781 atcttgcgtg gccaggagtt ttccaggtcg ttcagggtga tcggccgggt gcagcgccag
      841 ctcatgtagg tgttgaagtg atcatgcagg ttggaaccga ccgccggcat atcgcgcaca
      901 accgggacgc ccatgtcctg caggtgctga gccggcccaa gcccggatag ttgcagaagt
      961 tgcggcgatc cgtagctgcc accggacacg atgacctcgc gacctgccag cgccacgcac
     1021 cggccttcgg tgcaaacata ctcaacgccg gacgcccggc cgtcggcgat tagcacccgc
     1081 gtggcatgcg cacccgtgcg gatcgtcagg ttcgcacgcg accgggcggg atccagatag
     1141 gccttcgcgg tggtccagcg acgccgattc gaggttgttg tctgataata gccgcagcct
     1201 tcctggacgg cgccgttgaa gtcgggatta cgcggcaggc cggcctgctc gcaggcggcc
     1261 agcaccgcgt cggacagttc gaaacgcttc ggctgatctg acacgttcag cggacctccg
     1321 gcgccgtggt attcagaggc accgcggacc tggttctgtg ccttgcggaa gaacggcaag
     1381 actgaatccc agtcccatcc ggtgcatccg cgctgccgcc attcgtcgta gtcggcatga
     1441 ttgccgcgga tatacaccat gccgttgatc gaactggacc cgccgagggt cttcccgcgc
     1501 ggcagataga gccgccggtt ccccagggcc ggctgcggcg cactctcgaa cttccagttg
     1561 acggcgggat cgatgaaggt cttggcgaaa ccgagcggga tgtggatcca ggggttggtg
     1621 tcgggcggac cggcttccag caacagcaca cgatgccgcc cggattccga caggcgcgag
     1681 gcgaccacgg cgccagccga tcccgcgccg gtgacgatga agtcataggt ttcctgttgc
     1741 atggtccccc cggcctattc tcgttgatcg agtgtgggcc gggggtgccg cgcggagcaa
     1801 gagtgtcgca tagtggctcg cgacaggagt aagcccatgc ttgaagtcga tcatcccgcg
     1861 gacatgaagg cctacatcgg caagccggtc ggtagcagcg agtgggcact ggtcgatcag
     1921 acgatgatcg atgccttcgc caatgcgacc ggcgatcacc agtggatcca tgtcgatgtc
     1981 gatcgtgcga agaaggaaat gccgaacggc acgaccattg cccacggcta tctgacgctg
     2041 tcgctgctgc cccggttggg cgcgagcatt t
//
LOCUS       DASWQN010000570         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1902589, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000570 DASWQN010000000
VERSION     DASWQN010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902589"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1278
                     /locus_tag="VGC82_09535"
     CDS             256..1278
                     /locus_tag="VGC82_09535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0423549.1"
                     /translation="MAKPLRMGVVYDFRNPPESGMKTPDFYAAVLEQVTWLDSLGLDL
                     VWFTEHHFVDDGYLPSWIPVASAMASRTRHVRFSCDVCLLPFNHPIRLAEDLAVLDNI
                     SGGRVEIGVGLGYAAHEFRGFGLPVSHRLSYTDEGLEVLTRAFTGERFSFRGKRYDFQ
                     DVRIRPGYVQPGGPPLWIAALAEAGALRAARFDAHLLPQGPRTRSLDRWQTALNEAGR
                     DPGQHRVGIIRSCLVSDDAAHDFVPIREAERRRMALYNEFRAVSGGHGGVAGITQEQR
                     IPQSWIVGNLDECLAQMAAFIREYGFTDVVCWAVPPGLRPDQMNRHLERYATELVPRL
                     KAMFPG"
BASE COUNT          269 a          465 c          485 g          323 t
ORIGIN      
        1 ctgtttccgt tctcgtgcag ggagcgggcg ggtaagcggt cgggccacag gcgacccgcg
       61 tctgtcccgc ttttgcggtg ccgtgttgcc gtcgaaggta ccttttgatc cgcggttaga
      121 tgctgcggaa cgaattgtca cctctgcact ggtagacccg gtctggctgc ggcctccact
      181 ctcggcgctg gcttgcctag gcctgatttg ccacgcagat tgaggtcaag ggcaaaccgg
      241 aaggaaacgg gcatcatggc aaagccgctg cgcatgggcg tggtctacga cttccggaac
      301 ccgcccgagt ccggcatgaa aacccccgat ttttatgccg ccgtcctcga acaagtcaca
      361 tggctcgata gcctcgggct cgacctcgtc tggtttacag aacatcattt tgtcgacgat
      421 ggatacctgc cgtcatggat tcccgtggcg agtgcaatgg catcgcgcac ccgccacgtg
      481 cggttctcct gtgacgtctg cctgttgcca ttcaaccacc cgatccgcct tgccgaagac
      541 cttgccgtgc tcgacaacat cagcggcggc cgggtagaaa tcggcgtcgg cctcggttat
      601 gcggcacatg agttccgtgg cttcggcctg ccggtttcgc atcggctttc ctatactgac
      661 gaggggctgg aggtgctgac acgggccttt accggcgagc ggttctcatt ccgtggcaag
      721 cgctatgact ttcaggacgt gcggatccgt ccgggctatg tgcagccggg tggcccaccg
      781 ctttggatcg cagccctggc tgaagcagga gcgctgcgtg ccgcacgttt cgatgcccat
      841 ctattgccgc aggggccgcg gacgcgttca cttgatcggt ggcaaacggc gctgaacgag
      901 gccggtcggg atccgggaca gcatcgtgtc ggcatcatcc gctcctgcct tgtcagcgac
      961 gacgcagcac atgactttgt gccgattcgt gaagctgagc gtcggcgcat ggcgctctac
     1021 aacgagttcc gcgccgtgtc gggcgggcac gggggcgtgg ccggcatcac gcaagagcag
     1081 cggatcccgc aaagctggat cgtcggcaat ctcgatgaat gtttagcgca gatggccgcg
     1141 ttcatccgcg aatacggctt taccgacgtc gtctgctggg cggtgccacc tgggctgcgg
     1201 ccggaccaga tgaaccgcca cctagagcgt tacgcgaccg agttggtgcc tcggttgaaa
     1261 gcgatgttcc ctggctgatc aggccatcat caacctgcct tagcttctcc gatgataggg
     1321 cgggtgcgtc ggacccgaca ggctcaagtc ctggcttact cagcgatcgc taggttaggc
     1381 gacacgtgac gcaggaaaac ccagcttggc gctatgacgt ttttgctggt tggtcggcca
     1441 agctaccgac tgctgagtaa tcaccccctt aacggcaaac gttccggggg tggcgaactg
     1501 aagacgaatt ggccaccaag cacggcgcga ttaggctaat tc
//
LOCUS       DASWQN010000571         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_582807, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000571 DASWQN010000000
VERSION     DASWQN010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582807"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VGC82_09540"
     CDS             <1..466
                     /locus_tag="VGC82_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0423550.1"
                     /translation="DTLEANVRLARPDASQEQVSLALSQAALSGFVAGLPDGLATRVG
                     ERGVQLSGGQRQRVAIARAFLKDAPLLILDEATSHLDAISEQAVRGALDTLMADRTTI
                     VIAHRLSTIRAADLILVLRDGEVIESGSHDALLARGGFYARLVEHQMAGVAA"
     gene            640..777
                     /locus_tag="VGC82_09545"
     CDS             640..777
                     /locus_tag="VGC82_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423551.1"
                     /translation="MKNLILAAVAALGLATTVVPAANASSTVAGDAQATRTQQTGSYS
                     R"
     gene            complement(967..2061)
                     /gene="ychF"
                     /locus_tag="VGC82_09550"
     CDS             complement(967..2061)
                     /gene="ychF"
                     /locus_tag="VGC82_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY0423552.1"
                     /translation="MGFNCGIVGLPNVGKSTLFNALTATAAAQAANYPFCTIEPNVGR
                     VGVPDPRLDVLAGIGKSIKIVPTTLEFVDIAGLVRGASKGEGLGNQFLANIREVDAII
                     HVLRCFEDSDITHVEGSIDPLRDIEVVETELMLADLDSLERRLFQAQKRARGGDKESI
                     ASVAVMEPLVAALQEGKAARTAIPPGEEEAVKRLQLLTSKPVLYVCNVEEASAATGNA
                     FSDRVRAWAATQHAPAIVVSAAIEAEVSQLPEADRKDFLDTMGLSQSGLDQVIRAGYD
                     LLKLVTYFTVGPKETRAWTITNGTRAPQAAAVIHKDFERGFIACETIAYDDFVAYRGE
                     QGAKEVGKMRIEGKTYVVKDGDVLVFRFNV"
     gene            complement(2090..>2318)
                     /locus_tag="VGC82_09555"
     CDS             complement(2090..>2318)
                     /locus_tag="VGC82_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY0423553.1"
                     /translation="SMDKMLGGQDYWRVRLGIGHPGSKERVLGYVLGNFDADERAWLI
                     ALLDAVADAAGLLAQGKPAEFMTNVALHTAR"
BASE COUNT          401 a          778 c          692 g          447 t
ORIGIN      
        1 cgacacgctg gaagcaaatg tccggctggc ccgaccggac gcgtcacagg aacaagtctc
       61 gctcgcactg tcgcaggccg cgctatccgg tttcgtcgcc gggctgccgg atggcctggc
      121 cacccgtgtt ggtgagcgcg gcgtgcagtt gtcgggtggc cagcgccaac gggtcgcgat
      181 tgcccgggca ttcctgaagg atgcgccgct gctgatcctg gacgaggcga catcgcatct
      241 cgacgccatc agcgaacagg cggtgcgcgg tgccttggac acgctgatgg cggatcgcac
      301 cacgatcgtt atcgcgcatc ggctttcgac gatccgagct gcagatctga ttctggtgct
      361 gcgggacggc gaggtgatcg aatccggcag ccacgacgct ctgcttgcgc gcggcggctt
      421 ttacgcccga ctggtcgagc accagatggc gggtgtcgcg gcctgacccg gcgcggcatg
      481 ccgcggcggc gtcgcgactg gaacgggagc agcatccgta ttcatgcgct ggacgcataa
      541 attacatgcc gggaagccgg attatctttg ccgaagtgct ggtctatcta ccactccatc
      601 gaagccggcc tttcatccgg cgagtcatgg agaaattcga tgaaaaacct gattcttgcc
      661 gcggtcgcgg cccttggcct ggcgaccact gtcgtccccg ctgcgaatgc cagcagcacc
      721 gtcgccggag atgcgcaggc cacgcggacg cagcagaccg gctcgtatag ccgctaaatc
      781 attcactgag ggcttgctat cgaacgcggg ccctcagctt tgattgctcc gaaggcccgg
      841 gatcgctcgc ttcatccgga tattccctgc cgacccgggc tgccagacca tggcggccta
      901 ccgtgcaggc cccctatctc cttgttggtc gcatgattcg gccgttccgc ccagcgtgat
      961 cacgctctaa acattgaagc ggaacaccag aacgtcgccg tccttcacca cgtaagtctt
     1021 tccctcgata cgcatcttgc cgacttcctt ggcgccctgt tcgccccgat atgcgacgaa
     1081 gtcgtcgtag gcgatcgtct cgcaggcgat aaatccccgc tcgaaatcct tgtgaatgac
     1141 cgcggcggcc tgtggcgcac gggttccgtt ggtgatcgtc caggcgcggg tttctttcgg
     1201 accgacggtg aaatacgtca ccagcttcag caggtcgtag cccgctcgga tgacctgatc
     1261 caggccgctt tgcgacaagc ccatcgtatc caggaaatct ttgcgatccg cttccggcag
     1321 ctgcgaaacc tccgcctcga tcgccgcgga caccacgatg gccggcgcgt gctgcgtcgc
     1381 ggcccatgcg cgaacccggt cggaaaacgc gtttcccgta gcggccgagg cttcctcgac
     1441 gttgcataca taaagcactg gcttggacgt cagcagttgc agccgcttca ccgcttcctc
     1501 ctcgccaggc gggatcgcgg ttctggcggc tttgccctcc tgcagggccg cgaccagcgg
     1561 ctccatgacc gccaccgaag cgatactttc cttgtcgccg ccacgagccc gcttctgcgc
     1621 ctggaacaac cgccgctcca ggctgtccag gtcggccagc atcaactcgg tctccacgac
     1681 ctcgatgtcg cgcagcgggt cgatggagcc ttccacatgt gtgatgtcgc tgtcctcgaa
     1741 gcaccgcagc acatggatga tcgcatccac ctcgcgaatg ttcgcgagaa actggttgcc
     1801 gaggccttcg cccttggacg ctccgcgcac cagcccggcg atatcgacga attccaaagt
     1861 ggtcggcacg attttgatgg atttgccgat cccggccagc acatcgagcc ggggatcggg
     1921 cacgccgacg cgccccacat tcggctcgat cgtgcaaaac gggtagttgg cggcctgtgc
     1981 cgcggcggtc gccgtcaggg cattgaaaag cgtcgatttg ccgacgttgg gcagcccgac
     2041 gatgccgcag ttgaaaccca tcagcctgta tccctggtta gttcaagtgt cagcgtgcgg
     2101 tgtgcaacgc gacattggtc atgaattccg ccggcttacc ctgtgccagc aaacccgcgg
     2161 catcggccac cgcatccagc agcgcgatca gccacgccct ttcgtccgcg tcgaagttcc
     2221 ccagcacgta gcccagcacg cgttccttcg aaccgggatg accgatgcca agccgcacgc
     2281 gccagtagtc ctggccgccc aacatcttgt ccatgctg
//
LOCUS       DASWQN010000572         3024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2166253, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000572 DASWQN010000000
VERSION     DASWQN010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166253"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..587)
                     /locus_tag="VGC82_09560"
     CDS             complement(54..587)
                     /locus_tag="VGC82_09560"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY0423554.1"
                     /translation="MRIAAIIVATALAIPMAHAQAPTAAPSPAPVPEVMPFDIPYGLS
                     INLEQAQKAIAAAAAEAKKHNWKMSITVVGPAGDLVAHETMDGTQYASIDISQAKART
                     AAIYRRASGLFQAAINTNNSPSTLSLLAITHGAASEGGFPIVVDGKLVGAIGVSGGIF
                     TQDAATAKAGLEALGVK"
     gene            747..2465
                     /locus_tag="VGC82_09565"
     CDS             747..2465
                     /locus_tag="VGC82_09565"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0423555.1"
                     /translation="MHDTVIRGGTIVDGTGKAAYTGDVAMDGDKLAQVGGKAGPGKRE
                     IDANGLLVTPGWVDVHTHYDGQATWDPVLAPSSWHGVTTILFGNCGVGFAPVRNEDHS
                     ALIGMMESIEEIPGIALADGLKWNWETFPQYLDALDQMPRAIDIAAQIPHHPLRVYVM
                     GDRAINREPATGDDIAVMRDAVREGLKAGAFGFTTSRTNSHKTPSGDMVPGRYSEVDE
                     LLGIGSALKNMGYGAFGVNSDFDDETEELKWMTKLGQDTGRPVWFLLTDRPTDKVRWK
                     RLMAGVHQARADGAMVTAQIAGRPVGVMLGIDTALNPFSIRPSYQALLALPVAERIKR
                     MQDPAMRAQILSEAPSPELLNRLSQFRQHITTRWNRMFPMGDPPNYEPEEKDSVAAMA
                     ARSNHTPDEFAYDYLAAGAERFLFFPIVGYNEDNLDIIHTMLTDDATLLGLSDGGAHC
                     SSIIDASVPSWMLIHWARDRTRGPRLPLEMMVKRQTSETADFFGFHDRGRLAAGKKAD
                     VNVIDYEALRLHIPEVRYDLPMNGRRLVQRVDGYRNTFMSGVSTFENGIYSGATPGRL
                     VRARRI"
     gene            2552..>3024
                     /locus_tag="VGC82_09570"
     CDS             2552..>3024
                     /locus_tag="VGC82_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0423556.1"
                     /translation="MGQSSLEVPPITFGCNVFGWTADEKTSFALLDAWLDAGFNFLDT
                     ADVYSRWHPGNSGGDSEMIIGKWLRARSNRDKIILATKLGIEMAPGKKGLSKAYMQTA
                     VEDSLRRLQTDYIDLYQSHRDDPETPIEETLSAYADLIKQGKVREIGASNFSAAR"
BASE COUNT          565 a          902 c          977 g          580 t
ORIGIN      
        1 acgacaggcc ggttgggtcc gccatgcggc gggcccaacg tggttatgac cggctacttc
       61 acacctagcg cttcaaggcc ggctttggcg gtcgcggcgt cctgagtaaa gatcccgccg
      121 ctgacgccga tcgcaccgac cagcttgccg tccacgacga tcgggaaccc gccctcgctc
      181 gccgccccat gggtgatcgc gagaagactg agggtcgagg gcgaattgtt cgtgtttatc
      241 gcggcctgga acaagcctga ggccctgcga tagatcgcgg cggtgcgcgc cttggcctgg
      301 gaaatatcta ttgaggcata ttgcgtgccg tccattgtct cgtgcgcgac caggtctccg
      361 gctggaccca ccaccgtgat cgacatcttc caattatgtt tctttgcttc cgcagcggcg
      421 gcggcaatcg ccttttgcgc ctgctccaaa ttgatcgaga ggccataggg gatgtcgaac
      481 ggcatgactt ccggaaccgg tgccggggaa ggcgcggctg ttggcgcctg agcgtgcgcc
      541 atgggtatgg caagggcggt ggcaactatg atcgcggcga tacgcatgac cggggtccct
      601 catttatgtt cgccgttctc atccggctaa tgcttcctag ctgcgaaata gttccgggac
      661 aatatgccgg ccaagcccgg aaacttgacc gccgccgcac ggaggtttcc catagcgtcc
      721 cggaagaaca aaaacgggag caatccatgc atgatacggt gatccgaggc ggtacgatcg
      781 tcgatggtac cggcaaagcg gcctataccg gcgatgtggc gatggatggc gacaagctcg
      841 ctcaggttgg cggcaaggcc ggcccgggca agcgggaaat cgatgccaat ggcctgctgg
      901 tcacgcctgg ctgggtcgat gtccatactc attatgacgg tcaggcgacg tgggacccgg
      961 tgctggcccc gtcatcctgg cacggcgtca ccacgatcct gttcggcaac tgcggcgtcg
     1021 gtttcgcccc ggtgcgcaac gaggaccact cggcgctgat cggcatgatg gaatcgatcg
     1081 aggagatccc gggcatcgcg ctggccgacg gcttgaagtg gaactgggaa accttcccgc
     1141 agtacctcga tgcgctggac cagatgccgc gcgcgatcga tatcgccgcg cagatccccc
     1201 accatccgct gcgcgtgtat gtcatgggcg atcgcgcgat caaccgagaa ccggcgacgg
     1261 gcgacgatat cgccgtcatg cgcgatgcgg tcagggaggg cctgaaggcg ggcgccttcg
     1321 gcttcacgac gtcgcgcacc aactcccaca agaccccgtc cggcgacatg gttcccgggc
     1381 gctattccga ggtcgacgag ttgctgggga tcggttcggc cctcaagaac atgggctatg
     1441 gggcgttcgg cgtgaacagc gacttcgacg acgagaccga ggaactgaag tggatgacca
     1501 agttgggcca ggacaccggc cgcccggttt ggttcctgct gactgaccgg ccgaccgaca
     1561 aggtccgctg gaagcggctg atggcgggtg tccatcaggc tcgcgccgac ggtgccatgg
     1621 tgaccgccca gattgccggc cggccggtcg gcgtgatgct ggggatcgat acggccttga
     1681 acccgttttc catccggccg agctatcagg cactgctggc attgccggtg gcggagcgga
     1741 tcaagcggat gcaggatccg gcgatgcgag cacagattct gtcggaagcg ccttcgccgg
     1801 agttgctgaa ccggttgtcg cagttccgcc agcatatcac gacgcgctgg aaccggatgt
     1861 tcccgatggg cgacccgccg aactacgagc ccgaagaaaa ggacagcgtc gccgccatgg
     1921 ccgcccggtc caatcatacg ccggatgagt tcgcctatga ctacctcgcc gccggagcgg
     1981 aacggttcct gttctttcca atcgtcggct acaacgagga caacctggac atcatccaca
     2041 cgatgctgac ggacgatgcg acgctccttg gtctgtccga tggcggcgcg cattgctcgt
     2101 cgattatcga cgcaagcgta ccgagctgga tgctgatcca ttgggcgcgg gatcgcacgc
     2161 gcggtccgag gttgccgttg gaaatgatgg tgaagcggca gaccagcgag acggcggact
     2221 tcttcggctt ccacgaccgc ggccggttgg cggcgggcaa gaaggcggac gtcaacgtca
     2281 tcgactacga agcccttcgc ctccatatcc cagaggtccg ttatgacctg ccgatgaatg
     2341 ggcgtcgtct ggtacagcgg gtcgatggtt accggaatac gttcatgtct ggggtctcga
     2401 cgtttgagaa tggcatctat tcaggtgcca cgccaggacg gttggtgcgc gcccgccgta
     2461 tttgagttac attggatcga cctgttgggc ggtgccctcg ggtgccgccc atttttacgc
     2521 gcgcagggga tgaatgatgg aaactcgcag attgggtcaa tcctcgttgg aggtgccgcc
     2581 gattaccttt ggctgcaacg tgttcggctg gaccgccgac gagaagacat cgttcgccct
     2641 gctggacgcc tggctcgatg ccgggttcaa ctttctagat acagccgatg tttattcccg
     2701 ctggcatccc ggcaacagcg gtggcgactc tgaaatgatc atcggcaagt ggcttcgggc
     2761 tcgtagcaac cgcgacaaga tcatcctggc gaccaagctg gggatcgaga tggcgccggg
     2821 gaagaagggt ctttccaagg cctatatgca gaccgctgtt gaggattcct tgcgccgcct
     2881 gcagaccgac tacatcgatc tataccagtc gcaccgggac gatcccgaga cgccgatcga
     2941 agaaacgttg tccgcctatg ccgatctgat caagcaaggc aaggttcgcg agatcggcgc
     3001 ttcgaatttc tcggccgctc ggat
//
LOCUS       DASWQN010000573         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1770397, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000573 DASWQN010000000
VERSION     DASWQN010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770397"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VGC82_09575"
     CDS             <1..1059
                     /locus_tag="VGC82_09575"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423557.1"
                     /translation="PEDPKVITQIPVSAPQLRCNNLGLSGTTMTVAHQTDKAGQAGAG
                     MEVFDVADPAQPRKLSYFDTSGPHSRGVHYLWFADGHYAYLSTGAKDFTPKNKFDDQF
                     FMVVDVSDPKHPKEVGRWWMPGTRVGDQEPAPPRVGIDSGIRMHTPIVSADRPDRAYV
                     GWIDGGLVILDIADKAHPKLVARRSWQSQNVGFAHTLLPIPSRGLAIQTEEAVETNCK
                     DWPKRDWVWDISDEKAPIALSVMPPPADFAALCKQGGRFGAHNIAQNRPDPTVRNLNK
                     SVVGSFFNGGVRAYSIADPHDPKEIGFIVDAPPKGNASHSIQINDVYVDENGLIYAND
                     RLSGGLDIIRYTGPVPLE"
     gene            1065..1364
                     /locus_tag="VGC82_09580"
     CDS             1065..1364
                     /locus_tag="VGC82_09580"
                     /inference="COORDINATES: protein motif:HMM:NF023701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HEY0423558.1"
                     /translation="MPGRRFRQRGLAEQRDEGAEAGSFVGKTSCRRTQPLRFLVFLIL
                     DREPAILLHATGVLVSVPAPQRFVLHKLIVARRRRQVTRNGTRICFKLRLYWISW"
     gene            complement(1500..2213)
                     /locus_tag="VGC82_09585"
     CDS             complement(1500..2213)
                     /locus_tag="VGC82_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0423559.1"
                     /translation="MASDIVLVHSSDLHVDDDPHQGPYGEDGTTGLKVVLNAAKAHNA
                     DYVLLVGDIFDNNRQSPAIVAQCVALLQDVARPVIVLPGNHDPVTPDSVWLRGGLNTL
                     PNVHIIGVTHDTTVYFPEHDLEVWGHAHVDYSNMVPLRDPRMRTARWHVTLAHGHYED
                     DGVPPLRPSWLISNAEIEATRADYLALGHWNRAAEVGTGTTVPAYYSGSPDLAKTVNI
                     VRLRGNGTVEVVREAIEWD"
     gene            2340..>2943
                     /locus_tag="VGC82_09590"
     CDS             2340..>2943
                     /locus_tag="VGC82_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0423560.1"
                     /translation="MIDLHYWPTPNGKKVTILLEEAAIPYRIVPVNIGRGDQFTPEFL
                     KMNPNHRMPVMVDTTPAGGGEPISIFESGAIMLYLAEKVGRFYPQDLRGKYAVTEWLI
                     WQMANQGPKLGECGHFRRLGDKQGDQSYAVRRFTDEANRLYGVLNNRLYQSKYLAGDE
                     YTIADMISYPWTVNWKAQGQDIEEFKYVKRWQEELAARPAV"
BASE COUNT          600 a          942 c          863 g          538 t
ORIGIN      
        1 cccgaggacc ccaaggtgat cacgcagatt ccggtttccg cgccgcagtt gcgctgcaac
       61 aacctgggtc tgtcgggtac gacgatgacc gtggcacacc agaccgacaa ggcgggacag
      121 gccggggcgg gaatggaggt attcgacgtt gccgatcccg cacagccgag aaagctctct
      181 tacttcgata cgtcaggccc gcattcgcgc ggcgtgcatt atctttggtt cgccgacggc
      241 cactacgcct acctatccac cggggccaag gattttacgc ccaagaacaa attcgacgac
      301 cagttcttca tggtcgtcga tgtaagcgac ccgaagcatc cgaaggaggt cggccgctgg
      361 tggatgccgg gaacacgggt gggggaccag gaaccagcgc cgccaagggt gggcatcgac
      421 tccgggattc gcatgcacac gccgattgtt tcggctgacc ggccagaccg agcctatgtc
      481 ggctggatcg acggcgggtt ggtgatcctg gatattgccg acaaggcgca tcccaagctg
      541 gtcgcgcgcc ggtcctggca gtcgcaaaac gtcggttttg ctcatacctt gctgccgatc
      601 ccgtcccggg gattggcgat ccagaccgag gaagccgttg aaaccaactg caaggactgg
      661 ccgaagcgcg actgggtctg ggacatctcc gacgagaagg caccgatcgc gctgtccgtc
      721 atgccgccgc cagccgactt tgccgcattg tgcaagcaag gcggccgctt cggcgcgcac
      781 aacatcgccc agaaccggcc cgacccgacg gtgaggaatt taaacaaatc tgtcgtcgga
      841 agcttcttca atggtggcgt gcgggcttat tcgatagccg atccgcacga cccgaaggag
      901 atcggcttca tcgtcgatgc gccacccaaa ggcaatgcgt cccacagcat ccagatcaac
      961 gacgtgtatg tcgatgagaa cggactgatc tacgccaacg accgcttgag cggtggcctg
     1021 gacatcatcc gctacaccgg gcccgtcccc ttggagtaaa tccgatgccg ggccgtcgct
     1081 tccggcagcg cgggctcgcg gagcaacgcg atgagggtgc cgaggcaggc agctttgttg
     1141 gcaagacatc ttgtcgcaga acacagccat tgcggttcct ggttttcctt atcctcgatc
     1201 gggaaccggc gatcttactg catgcgaccg gcgtgctggt atccgtgccg gcgcctcagc
     1261 gcttcgtcct gcacaagttg attgtcgcgc ggcgcaggcg gcaggtaacc cggaacggga
     1321 caaggatctg tttcaagctc aggctctact ggatctcctg gtaagccgcc ggcctttcga
     1381 cggcctgctg cagactcttg ccacgtccga acgggaaggt tgattatgga ggatcaccgg
     1441 cacgggcccg ccaggacaaa acggccgtca atccaataga ttctggagtt gacctcggcc
     1501 taatcccact cgatcgcttc ccgaacgacc tccaccgtgc catttccgcg cagccgcacg
     1561 atgttcacgg tcttcgccag atccggtgac cccgagtaat acgccggcac cgtcgtcccg
     1621 gtccccacct cggccgcccg gttccaatgg cccagtgcca ggtaatcggc tcgcgtcgcc
     1681 tcaatttcgg cgttgctgat caaccatgac ggccgcaacg gaggaacccc gtcatcctcg
     1741 tagtgcccat gcgccagtgt cacatgccac ctggcggttc gcatcctcgg gtcgcgcaac
     1801 ggcaccatgt tgctgtagtc gacatgcgca tgtccccaga cctccaggtc atgctccggg
     1861 aaatacactg tcgtgtcgtg tgtcacgccg atgatatgga cattcggcag cgtgttcagc
     1921 ccgccgcgca gccaaaccga gtccggtgtc accggatcat ggttccccgg cagaacgatc
     1981 accggccgcg cgacatcttg cagcagagcg acgcattgcg cgacgatcgc gggcgattgg
     2041 cggttgttgt cgaagatatc gcctaccagc agcacgtaat cggcattgtg cgccttggcc
     2101 gcgttcagca cgaccttcag gccggtggtg ccgtcctcac cgtagggacc ttgatgcgga
     2161 tcgtcatcga cgtgaaggtc cgaactgtgc acgagcacga tatctgaagc caatgtcctc
     2221 aatccatcca actgccgacg acatcagacc ggggcggccc cgatccgtca accgtcattg
     2281 gcccggcaaa ggttgtcggc taccatggga ccaacaacaa catcgaggaa ccagacgtca
     2341 tgatcgacct ccattactgg cctacgccca acggcaagaa ggtcacgatc cttctggaag
     2401 aagccgcgat tccctacagg atcgttccgg tgaacatcgg ccgcggcgac cagttcacgc
     2461 ccgagttcct gaaaatgaac cccaaccacc gcatgccggt gatggtggac accaccccag
     2521 ccggcggggg cgagccgatc tccatcttcg aatccggcgc gatcatgctg tacctggccg
     2581 agaaggtcgg ccgcttctat ccgcaggact tgcgcggcaa atatgctgtc accgaatggt
     2641 tgatctggca gatggccaac caaggcccca agctcggcga gtgcggccac ttccgccgcc
     2701 ttggcgacaa gcagggtgac cagtcctacg ctgtccgccg cttcaccgac gaggccaacc
     2761 ggttatatgg cgtgctgaac aaccgcctgt atcaaagtaa atatttggcc ggcgacgagt
     2821 acacgatcgc cgacatgatc agctatcctt ggacggtgaa ctggaaagcc cagggccagg
     2881 acatcgagga attcaaatac gtgaagcgct ggcaagagga actcgcagcc cgtcccgccg
     2941 tcc
//
LOCUS       DASWQN010000574         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_713969, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000574 DASWQN010000000
VERSION     DASWQN010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713969"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VGC82_09595"
     CDS             <1..1134
                     /locus_tag="VGC82_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HEY0423561.1"
                     /translation="PMYGDLVGRWLAAKQGRSFKCLVLDLDNTVWGGVIGDDGLEGIA
                     LGQGSPLGEAYTAFQEYARELSRRGVILAVCSKNDEANALEPFEKHPDMVLRRGDIAC
                     FIANWQNKADNIRAIAQELNIGIDSLCFIDDNPFERNLVRQELPMVAVPEVSDDPTGY
                     PVALADAGYFEGLSVTGEDRERTAQYQGNKARDALKAAVTDLPSYLRGLEMQLIWKHF
                     DKIGLQRIVQLINKSNQFNLTTRRYSDEDVIAVMADPDAFGLQLRLTDRFGDNGIIAV
                     IIGRLRTNQDLYIDTWLMSCRVLGRQVEPTTLNLIAAEAAKLGGKRLVGEYIATKKNA
                     MVSEHYARLGFTVTETDGAGGNRSFLDLRGFTPAETFIHVVEG"
     gene            1140..1385
                     /locus_tag="VGC82_09600"
     CDS             1140..1385
                     /locus_tag="VGC82_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0423562.1"
                     /translation="MATEAEIYNNLNEIFTDVFMRDDIKLSAELTAKEVKGWDSFKQI
                     EIIMASEEKWGIKFNTRELDALRCVGDLAKMVATKVG"
     gene            complement(1514..>1750)
                     /locus_tag="VGC82_09605"
     CDS             complement(1514..>1750)
                     /locus_tag="VGC82_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0423563.1"
                     /translation="LPVGLYLCIHIMEGEIATPMLLARRFTINPVAVILALIFWYWMW
                     GVAGAILAVPLLAITKIICDDLRPLRAFGHLLEG"
BASE COUNT          393 a          496 c          556 g          305 t
ORIGIN      
        1 ccgatgtacg gcgatctggt tggacggtgg ctggcggcca agcagggccg gtcgttcaag
       61 tgcctcgtgc tggatctgga caacaccgtt tggggcggcg tcatcggcga cgatgggctg
      121 gaaggcattg cactcggcca aggtagcccg ctgggcgagg cttataccgc cttccaggaa
      181 tatgcccgcg agctgagccg ccgcggcgtc atcctggcgg tgtgctccaa gaacgacgaa
      241 gccaacgccc tggaaccgtt cgagaagcac ccggacatgg tgctcaggcg cggcgacatc
      301 gcctgcttca tcgccaactg gcagaacaaa gccgacaaca tccgagccat cgcgcaggag
      361 ctgaacatcg gcatcgattc cctgtgtttc atcgacgaca acccgttcga gcggaatctc
      421 gtgcgccagg agctgccgat ggtggcggtg ccggaggtat ccgatgaccc aaccggctat
      481 ccggtcgcgc tggccgacgc cggctatttc gaagggctgt cggtgaccgg cgaggatcgc
      541 gaacgaactg cccagtatca gggcaacaag gcacgcgatg cgctgaaggc ggcggtgacc
      601 gacctgccgt cctatctgcg tgggctggaa atgcagttga tctggaagca tttcgacaag
      661 atcggtctgc aacggatcgt gcagctcata aacaagtcca accagttcaa cctgaccaca
      721 cggcgctaca gcgatgagga tgtcatcgcg gtgatggccg atccggacgc gttcggcctg
      781 cagttgcgac tgaccgaccg cttcggggac aacgggatca ttgcggtcat tatcggacgg
      841 ttacggacga atcaggattt gtacatcgac acatggctca tgagttgccg cgtgctgggg
      901 cggcaagtgg agccgacgac gttgaacctg atcgcggcgg aagcggcgaa actgggcggc
      961 aaacggttgg tgggcgagta catcgcaacc aagaagaacg ccatggtcag cgaacactat
     1021 gcccgcctcg gcttcaccgt cacggaaacc gatggcgcag gcggcaatcg ctcctttctg
     1081 gatctgcggg gatttacccc cgccgaaacc tttattcatg tggtcgaggg atgataacaa
     1141 tggcgacgga agctgaaatc tataacaacc tgaacgagat cttcacggat gtcttcatgc
     1201 gcgacgatat caagttgtcg gcggaactga cggcaaagga ggtgaagggc tgggacagct
     1261 tcaagcagat cgaaatcatc atggcgtccg aggaaaagtg gggcatcaag ttcaacaccc
     1321 gcgaactcga tgctctgcgc tgcgtcggcg acctggcgaa aatggtcgcg acgaaggtgg
     1381 gctagaagta tgatcgcgcc gggcggaatg tcggcggcgc gtgaatcatg cgatcacaca
     1441 aagaccatga tcgcgccgcg accatgattt cgccgggcgg aacgtcctcc cgcgcgtgat
     1501 catgcaacga acatcagccc tccagcaaat ggccgaacgc tcgtaaaggt cgtagatcgt
     1561 cgcagatgat cttggtgatc gccagcaacg gcactgccag gatcgcgccg gcgacccccc
     1621 acatccagta ccagaagatc aacgcgagga tcactgcgac cgggttgatg gtgaagcggc
     1681 gggccagcag catcggcgtg gcaatttcgc cttccatgat gtggatgcaa agatacaggc
     1741 caaccggcag
//
LOCUS       DASWQN010000575         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1243178, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000575 DASWQN010000000
VERSION     DASWQN010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243178"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /gene="napA"
                     /locus_tag="VGC82_09610"
     CDS             <1..1541
                     /gene="napA"
                     /locus_tag="VGC82_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HEY0423564.1"
                     /translation="TAGATQPIDFDAYAAFVKDYTLEKVSELTGVERGFLLELAELYA
                     DPKRKVMSLWTMGFNQHVRGVWANQLVYNLHLLTGKISEPGNSPFSLTGQPSACGTAR
                     EVGTFAHRLPADMVVTNPEHRKHAEEIWRVPNGIIPEKPGYHAVEQDRMLKDGKLNFY
                     WIQVNNNLQAAPNSSGETYPGYRNPANFIVVSDAYPTVTAMAADLILPAAMWVEKEGA
                     YGNAERRTHVWRQLVDAPGEARSDLWQMMEFSKRFTTDEVWPAEILAANPNYRGKTLF
                     DVLFRNGSVDKFPLSEIPAEYENRESKAFGFYAQKGLFEEYAEFGRGHGHDLASYDTL
                     HTNRGMRWPVVDGKETKWRYREGLDPYVKPGKGVDFYGNKDGRARIIAVPYEPPAESP
                     DKEFDIWLVTGRVLEHWHSGSMTMRVPELYKAFPGARVFMHAEDARARGINEGAEVRV
                     VSRRGEIKSRIDTKGRNRMPRGVVFVPWFDASQLINKVTLDATDPISKQTDFKKCAVK
                     IIPA"
     gene            1553..2023
                     /locus_tag="VGC82_09615"
     CDS             1553..2023
                     /locus_tag="VGC82_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HEY0423565.1"
                     /translation="MPLAGSLFGGMLVFVCGAIYAQNAPPNPPQIIPRVTGVASPMGD
                     VPAAPLARPVVDDKRLMRNYPEQPPIIPHSIDNYQLTLKTNRCLDCHRRQFTEGSGAP
                     MISITHFMDRDGQVLADVTPRRYFCTSCHVQQTDAQPLVPSTFQDMLLVEPGKK"
     gene            2033..>2614
                     /locus_tag="VGC82_09620"
     CDS             2033..>2614
                     /locus_tag="VGC82_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY0423566.1"
                     /translation="MQRVKALLIWFWRIASRPSAHLSLGFLALGGFVCGVMFWGAFNT
                     ALEVTNTEKFCTSCHEMRDNVFQELQHTPHFSNRSGVRATCPDCHVPHDWTDKIARKM
                     QASKEVWGKIFGTIDTRQKFQAHRLELAKNEWVRMKANDSLECRNCHSSVAMDFTKQT
                     RRAAEIHGKFLVTGDKTCIDCHKGIAHELPDMTG"
BASE COUNT          548 a          859 c          756 g          451 t
ORIGIN      
        1 caacggctgg cgcgacgcag ccgatcgact tcgacgccta tgctgccttt gtgaaggact
       61 acacgctcga gaaggtatcc gagctcacgg gcgtcgagcg cggtttcctg ttggaactcg
      121 ccgaactcta tgccgatccc aagcgcaagg ttatgtcgct ctggacgatg ggcttcaacc
      181 agcacgtacg cggcgtgtgg gccaatcaac tggtctacaa tcttcatctc ctgacgggga
      241 agatttccga acccggcaat tcgccgttct cactgacggg acagccatcc gcctgcggca
      301 cggcgcgcga ggtcggcacc ttcgcgcacc gcctgcccgc cgacatggtc gtcaccaatc
      361 cggaacaccg caagcacgcc gaggaaatct ggcgcgttcc gaacggcatc atcccggaga
      421 agcccggcta tcacgcggtc gagcaggacc ggatgctgaa agacggcaag ttgaacttct
      481 actggatcca ggtgaacaac aacctgcagg ccgcgccgaa tagctcgggc gagacctatc
      541 ccggctaccg gaatccggcg aacttcattg tcgtctccga cgcctacccg accgtaacag
      601 cgatggccgc cgatctgatt ctgccggcgg cgatgtgggt cgagaaggaa ggcgcctacg
      661 gcaacgcgga acggcgtact catgtctggc ggcagctcgt cgacgcgccc ggcgaggcgc
      721 gctccgatct ctggcagatg atggagttct ccaagcgctt taccaccgac gaggtgtggc
      781 ccgcggagat cctcgccgcc aatcccaact atcgcggcaa aacccttttt gacgttctat
      841 ttcgcaacgg cagcgtcgac aagttcccgt taagcgagat cccggccgag tacgagaacc
      901 gcgaatcaaa ggcattcggc ttttatgcgc agaagggcct gttcgaggaa tacgccgaat
      961 tcggccgcgg ccatggccac gatctcgcat cctacgacac actccacacg aatcgcggaa
     1021 tgcgctggcc cgtggtcgac ggcaaggaaa cgaagtggcg ctaccgcgaa ggtctcgatc
     1081 cctacgtcaa gccgggcaag ggcgtcgact tctacggcaa caaggacgga cgggcgcgga
     1141 tcatcgcggt gccctacgaa ccgcccgcgg aatccccgga taaggagttc gacatctggc
     1201 tggtgactgg ccgtgtgctc gaacattggc actccggctc catgaccatg cgggtgcccg
     1261 aactctacaa ggcgttcccc ggcgcccgcg tcttcatgca tgcggaagac gcgcgggccc
     1321 ggggcatcaa tgagggcgcg gaggtccgcg tggtttcccg ccgcggcgaa atcaagagcc
     1381 gcatcgacac caagggccgc aaccggatgc cacgtggcgt ggtcttcgtc ccgtggttcg
     1441 atgccagcca actcatcaac aaggtgacac tggacgcaac cgatcccatt tccaaacaga
     1501 cggacttcaa gaaatgcgcg gtcaagatca ttccggcctg aagagacttc gcatgcctct
     1561 ggcgggctca ctattcggcg gcatgttggt gttcgtctgc ggcgcgatct acgcccagaa
     1621 cgcacccccg aacccgcctc agatcattcc gcgcgtgacc ggagttgcct cgccgatggg
     1681 cgacgtgccg gcggcgccct tggcccggcc ggtcgtcgac gacaagcggc tgatgcgcaa
     1741 ttatccggag cagccgccca tcatcccgca ctcgatcgac aattatcagc tgacgctaaa
     1801 gactaaccgt tgcctcgatt gccaccgacg ccagttcacc gaaggctcgg gcgcaccgat
     1861 gatcagcatc acccatttca tggaccgcga cgggcaagtt ctcgccgacg tgacgccgcg
     1921 ccgatacttt tgcacctcct gtcacgtcca acagacggac gcccagccgc tcgtgccaag
     1981 cactttccag gacatgcttc tcgtcgaacc cggaaagaag tgagaagcat ccatgcagcg
     2041 agtcaaggca ctcctgatct ggttctggcg gatcgccagc cgcccaagcg cgcatctgag
     2101 cctgggcttc ctcgcactcg gcggtttcgt ctgcggcgtg atgttctggg gcgccttcaa
     2161 caccgcgctc gaagtcacca acaccgagaa gttttgcacc tcctgtcacg aaatgcgcga
     2221 caacgtcttc caggagttgc agcacacgcc ccatttctcc aaccgctcgg gcgtccgcgc
     2281 aacctgcccg gactgccacg ttccacacga ctggaccgat aagatcgccc gcaagatgca
     2341 ggcatcgaag gaagtctggg gcaagatctt cggcaccatc gacaccaggc agaagtttca
     2401 ggcccaccgt ctcgaactcg ccaagaacga gtgggtgcgg atgaaagcga acgattcgct
     2461 ggaatgccgc aactgccatt cctcggtggc gatggacttc accaaacaga cgcgacgcgc
     2521 ggcggaaatc cacggcaagt ttctcgttac cggggacaag acttgcatcg actgccacaa
     2581 gggcatcgca catgaactgc ccgacatgac cggc
//
LOCUS       DASWQN010000576         3641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1506508, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000576 DASWQN010000000
VERSION     DASWQN010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506508"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1465
                     /locus_tag="VGC82_09625"
     CDS             <1..1465
                     /locus_tag="VGC82_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY0423567.1"
                     /translation="FSGTGNATANVITGGTGNDTLDGGDGNDTLSGGLGADTMIGGAG
                     DDTFSVDNIGDSVVEGIAGGTDTVNSTLNSYTLAANVENLHFTGTGAFTGTGNTAANI
                     ITGGAGDDTLNDGGIGGTGDTLTGGAGNDTYFINNIGDVVGEAAGGGTDTVSTILNSY
                     TLGANVENLTFNGVGNFIGIGNTAANILTGGAGDDSLKSGGGNDTLIGGTGNDVLDGG
                     IGADTMIGGVGDDIYFVDNVGDAVTEAAGEGTDTVNTTLNSYVLGAAVENLKFGGTGN
                     FTGTGNTLANVITGGAGDDTLDDGGVGGAGDKLTGGTGNDIYLVRNSGDFIVEALNGG
                     TDTVDTTLLGYTLGANVENLAFIGGATGSFTGTGNALDNTIIGGASADKLSGGAGNDV
                     IIGGGGNDTLTGGTGNDSFAYLSADFGADIITDFGSSPGNADLIDISALGITAATFAA
                     SVTIGGTASTVLTIGASTISLTGVNSNTIDSTHFKLA"
     gene            1626..2204
                     /locus_tag="VGC82_09630"
     CDS             1626..2204
                     /locus_tag="VGC82_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423568.1"
                     /translation="MHRRISSLSGLVLLSGLAFGLAACSPLPVAQRAPFLPPGVYGTY
                     EDNDTGAINQSAWAFASPANTRGNPVDAAKAIVALEYLSGELRQNPRWVGLPASVKLH
                     MARARDELRLIVGIRPDASSQMAVNALLALALDLETENQPAAMAVLQSPVFIRPPEET
                     LRVLSNLPYVQEANLATARAEAGAFPVSGPNS"
     gene            complement(2247..>3641)
                     /gene="phaC"
                     /locus_tag="VGC82_09635"
     CDS             complement(2247..>3641)
                     /gene="phaC"
                     /locus_tag="VGC82_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEY0423569.1"
                     /translation="FVQDVVTHVDGLDAKTAQKVDFYSRQFIDAMSPSNFLMTNPEVL
                     RKTAETGGENLLKGLNNLLGDLERGKGQLRIKMTDTDAFKLGENIGVSPGKVVFQNDL
                     MQLIQYAPSTAKVLKRPLLIAPPWINKFYILDLRPRNSFVRWAVSQGHTVFMISWVNP
                     DETLAEKGFEDYMKQGYLAALDAIEAATGEREVNAIGYCLGGTLLASTLAYMAVKGDD
                     RIKTATFFVTMMDFQEAGELGVFIDEEQLQALEDKMNKRGYLEGSEMATTFNMLRAND
                     LIWSFVVNNYLMGNDPFPFDLLYWNADSTRMPARMHSFYLRKMYQENLLAKPNGIELD
                     GVPIDLGKIKVPAYFLSTREDHIAPWKSTYRGTQLLGGPKRFVLAASGHIAGVVNPPE
                     GGKYGHWISTELPAEPENWFQGATEMAGSWWTDWQRWILGSSKEQVSVRQPGDGKLKA
                     IEDAPGSYVQVRLS"
BASE COUNT          740 a         1162 c         1051 g          688 t
ORIGIN      
        1 cttctccgga accgggaatg cgaccgccaa tgtcatcacc ggcggcaccg gtaatgacac
       61 actcgacggc ggagacggga acgacacact gagcggtggt ctgggcgccg acacgatgat
      121 cggcggcgcg ggcgacgaca ccttctcggt tgataatata ggcgattctg tcgtcgaagg
      181 gatcgccgga ggcaccgaca ccgtcaacag cacgctgaac agctacacgc ttgcagccaa
      241 tgtcgaaaac cttcatttca ccgggactgg cgctttcacc ggcactggaa acaccgcggc
      301 gaacatcata acgggtggtg ctggtgacga cactctgaac gatggcggca tcggcggcac
      361 cggcgataca ctgacgggcg gcgcaggcaa cgatacttac tttatcaata atatcggcga
      421 tgtcgtcggc gaggctgccg gtggaggaac cgatactgtc tccacgatcc tgaatagcta
      481 cacgctcgga gccaatgtcg aaaatctcac gttcaacgga gtcggcaact tcatcggcat
      541 tggaaacacg gcggcaaaca tcctcaccgg cggcgccgga gacgacagcc tcaaaagcgg
      601 cggcgggaac gacacgctga ttggcgggac cggcaacgac gtcctggacg gcggcatcgg
      661 cgccgacacg atgatcggcg gcgtgggcga cgacatctac ttcgttgaca acgtcggcga
      721 tgccgtgacc gaagccgccg gcgaaggcac ggacacggtc aatacgacgc tgaacagcta
      781 cgtcctgggg gccgccgtcg aaaacctcaa gttcggcgga accggcaact tcaccggcac
      841 cggcaatacc ctggccaacg tcatcaccgg cggtgcgggc gacgacacgc tcgatgacgg
      901 cggtgtcggc ggtgctggcg acaagctgac tggcgggacc ggcaacgata tctacctggt
      961 ccgcaacagc ggcgacttta tcgttgaagc gctgaacggg ggcaccgata cggtcgacac
     1021 cacgctgctc ggctataccc tgggagccaa cgtcgaaaat ctcgctttta tcggcggcgc
     1081 aaccggcagt ttcaccggca ccggcaacgc tctggacaac accatcatcg gcggcgccag
     1141 tgcggacaaa ctaagcggtg gggcaggcaa cgacgttatt atcggcggcg gcggcaatga
     1201 cacgttaacc ggcggcaccg gcaacgacag ctttgcgtat ctcagtgcgg atttcggtgc
     1261 cgacatcatc accgatttcg gctccagccc ggggaatgcg gacttgatcg acatcagcgc
     1321 cctcggcatc acagccgcga cgtttgcggc ctccgtcacc atcggcggta cggccagtac
     1381 ggtgctaacc atcggcgcca gcaccatctc gctgaccggt gtcaactcga acacgatcga
     1441 cagcactcac ttcaagctcg cctgaacacg ccggagcggg ggccatgcca gagcattttc
     1501 agcctgccag tacaggctga aaatgctcta tctccagtgg catgtgtttg cgggcggcta
     1561 aatcgccctg gcgctgcgac cagcgcatgc caatctatgg cgcagagcga tcggagcccg
     1621 ccagtatgca tagacgaatt tcatcactat ccggcctggt tttactatcc ggcctggctt
     1681 tcggcctcgc cgcctgctcg cccctgcccg tggcgcagcg cgcaccgttt cttccgcctg
     1741 gcgtctacgg cacctatgag gataacgaca ccggcgccat aaaccagtcg gcctgggcct
     1801 ttgcgtcgcc tgccaacacg cgcggcaacc cggtggatgc cgcgaaagca attgttgccc
     1861 tggagtatct ttctggcgaa ctacgacaaa acccgcgttg ggtcggtctc ccggcgtcgg
     1921 tcaaattgca tatggcacgg gcacgcgatg agctacgtct gatcgtcggc atccgtcctg
     1981 atgcttcgtc ccagatggcc gtgaacgcgc tcttggctct cgccttggat ctggagaccg
     2041 aaaatcagcc ggcggcaatg gcggtgctgc agtcgcccgt gtttatccgc ccgcccgagg
     2101 aaacgttgcg ggtcctatcg aacctgccct acgtccaaga ggccaatctg gcgaccgcgc
     2161 gagccgaggc aggagcattc cctgtctcgg gcccgaactc ctgaggctgg gttccgcgcc
     2221 aaacacaacc tttcgccatc caaccactac gataaccgca cctgcacgta gctgcccggc
     2281 gcatcctcga tcgctttcaa cttcccgtcg ccgggttgcc gaacggacac ttgctctttg
     2341 ctggacccca ggatccaacg ctgccaatcg gtccaccagg aaccggccat ttccgtcgcc
     2401 ccctggaacc agttctccgg ttcggccggc agttccgtac tgatccaatg gccatatttc
     2461 ccgccctcgg gcgggttcac gacaccggcg atgtgacccg aggccgccaa cacaaagcgc
     2521 ttcgggccgc cgagtagctg ggtgccgcga taggtggatt tccagggcgc gatatggtcc
     2581 tcccgcgtcg acaggaaata cgccggcacc ttgatcttcc caagatcgat cggcacgccg
     2641 tcgagctcta ttccattcgg cttcgccagc aggttttcct gatacatctt gcgcagatag
     2701 aagctgtgca tccgcgccgg catccgggtg gaatccgcgt tccagtaaag cagatcgaat
     2761 gggaacgggt cgttgcccat caggtagttg ttcaccacga acgaccaaat caggtcgttg
     2821 gcgcgcagca tgttgaacgt cgtggccatc tcgctgccct cgagatagcc acgcttgttc
     2881 attttgtctt ccagcgcctg aagctgttcc tcatcgatga acacccccaa ctcgccggct
     2941 tcctggaaat ccatcatggt aacgaagaag gtggccgtct taatccggtc gtcacccttc
     3001 accgccatgt aggccaaggt cgatgccaac aatgtcccgc ccaggcaata gccgatggcg
     3061 ttgacctcac gctcgccggt ggccgcctcg atcgcgtcca gcgcagcgag gtagccctgc
     3121 ttcatgtagt cctcgaaacc tttctcggcc aaggtttcat ccggattgac ccaagaaatc
     3181 atgaagacgg tatgtccctg ggaaaccgcc cagcggacga aactgttccg cggacgcaga
     3241 tccaggatgt agaacttgtt gatccatggc ggcgcgatca gcaacggccg cttcagcact
     3301 ttcgcagtcg agggcgcgta ttgaatcaac tgcatcaggt cgttctgaaa cacgaccttg
     3361 ccgggggaca caccgatgtt ctcgccgagc ttgaacgcgt cggtgtcggt catcttgatc
     3421 cgcaactggc ccttgccgcg ctccaggtcg cccagcaggt tgttcaggcc cttcagcaag
     3481 ttctcgccgc cggtttcggc ggtcttacga agcacttctg gatttgtcat caagaagttg
     3541 gacggactca tcgcgtcgat gaattggcgg gaatagaaat cgactttctg ggctgtcttg
     3601 gcatccagcc catcgacatg cgtcacgacg tcctgcacga a
//
LOCUS       DASWQN010000577         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_978941, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000577 DASWQN010000000
VERSION     DASWQN010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978941"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VGC82_09640"
     CDS             <1..902
                     /locus_tag="VGC82_09640"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEY0423570.1"
                     /translation="LSELFEELPEAGDPPKPKLVFFFDEAHLLFSDAPKALMDKIEQV
                     VRLIRSKGVGVYFITQSPADVPDTVLGQLGNRVQHALRAFTPRDQKAVKAAAETMRAN
                     PKLDTATAIMELAVGEALISFLDAKGTPEITERAFVLPPTSQIGPITPQQRRDLIGSS
                     VVAGIYEKSVDRESAFEMLRGRAGGGQAVPPETHQAAAAAQSSAASVWNQGQVSPSAP
                     TAQPLPGSAWNQEKVPQAPAAQGGVWGGLSNILFGSTGPRGGYHEGAIDGLAKSAART
                     VGSAVAREVVRGVLGSLLGSGRR"
     gene            1206..1373
                     /locus_tag="VGC82_09645"
     CDS             1206..1373
                     /locus_tag="VGC82_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423571.1"
                     /translation="MQSRSIPPHLPGAFHADPGDRLIIATARHLGTPIVTGDRKILAY
                     AEAGFVRVIPC"
     gene            1457..2023
                     /gene="efp"
                     /locus_tag="VGC82_09650"
     CDS             1457..2023
                     /gene="efp"
                     /locus_tag="VGC82_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY0423572.1"
                     /translation="MKQQANLIRAGQVIEHEGRRWTVLKQQIITPGKGGAFIQVEMRD
                     LKSGNKTNERWRTADTVERLTTDQKEYTYSYTDGINLVLMDPETYEEAHIPADLMGDA
                     APFLQDQMKVTVDLVEGDAVAIHLPGSVTLEVVEADPVVKGQTASSSYKPAKLSNGVK
                     TNVPPFIEAGERIVVRTDDGSYVERAKG"
     gene            2023..>2832
                     /locus_tag="VGC82_09655"
     CDS             2023..>2832
                     /locus_tag="VGC82_09655"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0423573.1"
                     /translation="MAFRLSPHLTVMANAAQKASKRLLRDFAEVEQLQVSIKGPSDFV
                     SQADLRAEQTLKEELTKARPGYSFLMEESGASGSENWTWRWIVDPLDGTTNFLHGMPH
                     WAISIGLERRLPDGTSEIHAGLIYAPAADEMFWAEKGTGAFMNERRLRVSARREFNEA
                     VFATGIPFGAVSAPRQQAFSRTLTMLMPQVAGIRRFGAAALDLAWVAHGRFDGYWELG
                     IKPWDIAAGELLVREAGGYATDVGDGNPLATGNIVAGNPHLHAKLRAVVAEG"
BASE COUNT          525 a          902 c          916 g          489 t
ORIGIN      
        1 tgctgtcgga gctgttcgag gaactgcccg aggccggcga tccgccgaaa cccaaactgg
       61 tgttcttctt cgatgaggcg catctgttgt ttagcgatgc gccgaaggcc ctgatggaca
      121 agatcgagca ggtggtccgc ctgatccgct cgaagggcgt cggcgtctac ttcatcacgc
      181 agagcccggc ggacgtgccg gacacggtgc tggggcagct gggcaaccgc gtgcagcacg
      241 cgctgcgggc gtttaccccg cgtgaccaaa aggcggtgaa ggcggcggcg gagacgatgc
      301 gcgccaaccc gaagctcgat accgccacgg cgatcatgga gcttgccgtg ggggaggcac
      361 tgatctcgtt cctggacgcc aagggcacgc cggagatcac cgaacgcgcc ttcgtcctgc
      421 cgcccacgtc gcagatcggc ccgatcacgc cgcagcagcg ccgcgacctg atcggcagtt
      481 cggttgtcgc cggcatctac gaaaagtcgg ttgatcgcga atcagcgttt gaaatgttgc
      541 gtggccgtgc cggcggcggt caggcggtgc cgcctgaaac gcaccaggcc gccgccgcgg
      601 cccagtcatc ggccgcgtca gtgtggaacc agggtcaggt ctcaccatcg gcaccgactg
      661 cccaaccctt gcctggttcg gcgtggaacc aggagaaggt cccgcaagcg ccggccgccc
      721 aaggcggcgt atggggtggc ctgtcgaaca tcctgttcgg cagcaccggc ccccgcggcg
      781 gttaccacga gggggccatt gacggcctcg ccaagagcgc cgcccggacc gtgggcagcg
      841 ctgtcgccag ggaggtggtg cgaggtgtgc tcggctcatt gctggggagt ggccgacgct
      901 agaccatttt cagcctgact gtgcgggccg taaatggtga acctcattgt attatcacgc
      961 gatccacacg cttcggacgt tccgccagcg tgatcacgct ctagtttggt cttccgagtg
     1021 cggttgccga ttgccatcga tggtacgcgg ccaacagatc gacgtgctgg agagcgtggc
     1081 aatgatgcaa ttgccgagca ggcccaggct tggccgttct cccgccttgc aatgcgggcc
     1141 ggacccgaag acgtggttcg caagaaccct ggcgggtccg ggcatcaggg aggcgccgat
     1201 ctcggttgca atcgcgatcg attccgcctc atcttcccgg cgcattccat gccgatccgg
     1261 gcgaccgcct gattatcgcc accgcccggc atctcgggac gcccattgtt accggcgacc
     1321 gcaaaatcct cgcctacgcc gaggctggtt tcgtgcgagt catcccctgc tgagcagacc
     1381 cccacaacct tgccccaagc ccttccaggg tctagaagcc ggcacctttt ccaattccac
     1441 cagatggatc tacccaatga agcagcaggc gaacctgata cgcgccggcc aggtgatcga
     1501 gcatgaaggc cgccgctgga cggtgctgaa gcagcagatc attactcccg gcaagggcgg
     1561 cgcctttatc caggtcgaga tgcgcgatct gaaatcgggc aacaaaacca atgagcgctg
     1621 gcgtaccgcc gacaccgtcg agcggctgac caccgaccag aaggaataca cctactccta
     1681 taccgacggc atcaacctcg tgctgatgga cccggaaact tacgaggaag cgcatattcc
     1741 cgccgatctg atgggcgatg cggcgccgtt cctgcaggac cagatgaagg tcaccgtcga
     1801 tctggtggaa ggtgacgcgg tcgcgatcca cctgcccggt tcagtcacgt tggaagtcgt
     1861 cgaagccgat ccggtcgtca aaggccagac cgcatcgtca tcatacaagc cggcgaagct
     1921 gtccaacggc gtcaagacga acgtgccgcc gttcatcgag gccggagaac gtattgtggt
     1981 ccgcaccgac gacggcagct atgtggagcg ggcgaagggc tgatggcgtt ccgtctctcc
     2041 ccgcatctga ccgtcatggc caatgccgcc caaaaggcct cgaagcggct gctgcgcgat
     2101 ttcgcggaag tcgagcagtt gcaggtcagc atcaaaggcc cgtccgactt cgtctcccag
     2161 gccgatctgc gcgccgaaca aaccctgaaa gaggaactga cgaaggcacg ccccggctac
     2221 tcgttcctga tggaggaatc cggcgcgtcc gggtccgaga actggacctg gcgttggatc
     2281 gtggacccgt tggacggcac gaccaacttc ctgcacggca tgccgcactg ggccattagc
     2341 atcgggctgg agcgtcgcct gcctgatgga acctcggaga tccacgccgg tctgatttac
     2401 gctccggctg ccgacgagat gttctgggcg gaaaagggca ccggcgcgtt catgaacgaa
     2461 cgccgcctgc gcgtctccgc caggcgcgag ttcaatgagg cggtgttcgc cacggggatt
     2521 ccgtttggcg cggtctcggc gccccgccag caggcattca gccggacatt gaccatgttg
     2581 atgccacagg tcgccggcat ccgccgtttc ggtgccgccg cgctggatct tgcctgggtg
     2641 gctcatggcc ggtttgacgg atactgggaa ctgggcatca aaccgtggga catcgccgcc
     2701 ggcgaactgc tggtgcgtga ggcaggcggc tacgcaacgg atgtgggcga cggcaacccg
     2761 ctcgcgaccg gaaacatcgt ggccggcaac ccgcatctgc atgcgaaatt gagggctgtg
     2821 gtcgccgaag gt
//
LOCUS       DASWQN010000578         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2298956, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000578 DASWQN010000000
VERSION     DASWQN010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2298956"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VGC82_09660"
     CDS             <1..446
                     /locus_tag="VGC82_09660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0423574.1"
                     /translation="RRQPSRLMLLGTGPMLHELQALAGTLGIAEHVAFEGFVRNPLPY
                     MRAADGFVLSSRSEGFGNVLVEAMGCGTPIISTDCPHGPADILAHGKYGILVPPRDPE
                     ALAPAFGRLLAERAKWPAERLRERASAFSYEACADGYARLLQSLA"
     gene            573..989
                     /locus_tag="VGC82_09665"
     CDS             573..989
                     /locus_tag="VGC82_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEY0423575.1"
                     /translation="MATQGRALPQPTPETQHFWDGTKANELLLQRCDECSKVYFPPRP
                     FCPACASRKVSVTKASGKAKLYSYVIHHRPVPGFTPPYAIAVVELDEGPRMMTNIVEC
                     EQTPQALVLDMPVEVVFEPQTDTITLPFFRPAATKG"
     gene            994..2136
                     /locus_tag="VGC82_09670"
     CDS             994..2136
                     /locus_tag="VGC82_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HEY0423576.1"
                     /translation="MPHNTVAVVGAAETTRMGVIPELSQLGLHADAALNAMADCGLKP
                     SDIDGIATAGETPVQLAHYLGITPTWVDGTSVGGCSFMIHVRHAAAAIESGLAKTILI
                     THGESGKSRVGNPPRSVHPQSLNGQFEQPFGPMGPPSMFTIPVLRYMKTYGVTEEQIA
                     QVAVVQREWAAKNPRATFRNPITTDDVLNSRMIAYPFRLLMCCLVTDGGGALILTSAD
                     RAKDFPTRPVYILGTGESAETPMVSQMEDFTSSRAFRVAGPTAFKEAGITHKDVDHLM
                     IYDAFAHLPIYGLEDIGFLPKGEAAAFIAGRNTAPGGSLPLNTNGGGLSYMHSGMYGM
                     YALQESVRQMRGTAPAQIAGAKISVCHGVGGMFAASGTIIFSNQAP"
     gene            2208..>2892
                     /locus_tag="VGC82_09675"
     CDS             2208..>2892
                     /locus_tag="VGC82_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0423577.1"
                     /translation="MTESLFTGLKVIDCASWIAGPAAATMMSDFGADVIKIEPPGAGD
                     PWRASTPIPGKPVDYYWQLTSRNKRSLALDLKHPDGQAVLYRLVASADVFITNYPLPV
                     RDRLKLAPADLLPLNPRLIYASFTAYGEAGEEAAKTGFDSTAYWARTGLMDMVRASPD
                     TVPSRSMPGMGDFPSATGVYAAIVTALYRREKTGQGGVVRSSLLQSGLWANGCAVQTR
                     LFGEDVGLRP"
BASE COUNT          510 a          892 c          973 g          517 t
ORIGIN      
        1 tccgccgcca gccctcccgg ttgatgctgc tgggaaccgg gccaatgctg catgagctgc
       61 aagccctggc aggcacactg ggtatcgccg aacacgtcgc gttcgaaggc tttgtgcgaa
      121 atccgctgcc gtacatgcgg gcggcggacg ggttcgtgct ctcctccagg tcggaaggat
      181 ttggcaacgt cctggtggaa gccatgggat gtgggacgcc gatcatctcg accgactgcc
      241 cgcatggtcc ggccgatatc ctggcgcatg ggaaatacgg catcctggtt ccgccgcgcg
      301 atcccgaagc gctcgcgccg gcgttcgggc gcctgctggc ggaacgggca aagtggcccg
      361 cggagcgtct gcgcgaacgg gccagtgcgt tcagctacga agcctgcgcg gacggatatg
      421 cgcggctgct gcaaagtctg gcctgaggca ccgcgggttc gccgttcggc tggcggtttc
      481 aggctggttg cccgcaatcg aacggctgcg gcaagttgcc catagggccg ctccagccta
      541 accttgctcc catcagaccg gaggaaccga cgatggcgac acaaggacgc gcattgccgc
      601 agccgacacc cgagacgcag catttctggg acggcaccaa ggcaaacgaa ctgctgctgc
      661 agcgatgtga cgagtgcagc aaggtgtatt ttccaccgcg gccgttctgc ccggcgtgcg
      721 catcgcggaa ggtttcggtg accaaggcca gcggcaaggc aaagctgtac agctacgtca
      781 tccatcaccg cccggtgccg gggtttactc cgccctacgc catcgccgtc gtggaactgg
      841 acgagggccc acgcatgatg accaatatcg tggagtgcga gcaaacgccc caggcgctgg
      901 tcctggacat gcccgtcgag gtcgtgttcg agccgcagac cgatacgatc acgctgccct
      961 tcttccgtcc cgccgcaacg aaaggctgat ccgatgcctc ataacacggt tgccgtcgtc
     1021 ggtgcggctg aaaccacgcg gatgggggtt atccccgaac tgtcgcagtt gggactgcat
     1081 gccgatgcgg ccttgaacgc gatggcggat tgcgggttga agccgtccga catcgacggg
     1141 atcgcaacgg ctggcgaaac gccggtgcag ctcgcgcatt accttggcat cacgccgacc
     1201 tgggtcgatg ggacgtcggt cggcgggtgc agcttcatga tccatgtccg ccacgccgcg
     1261 gcggcgatcg agtccggcct ggcgaaaacc atcctgatca cgcatggcga gtcgggaaaa
     1321 tcgcgggtcg gcaacccgcc tcgttcggtg catccgcagt cgctgaacgg ccagttcgag
     1381 cagccgttcg ggccgatggg gccgccgtcg atgttcacca ttcccgtgct gcgctacatg
     1441 aagacctatg gcgtgaccga ggaacagatt gcgcaggtcg ccgtggtgca gcgcgaatgg
     1501 gcggcgaaga acccgcgggc gacgttccgt aacccgatca ccaccgatga cgtgctgaac
     1561 agccgcatga tcgcctatcc gttccggttg ctgatgtgtt gcctggtgac cgacggcggc
     1621 ggcgcgctga tcctgacctc ggcggatcgg gcgaaggatt tcccgaccag gccggtctat
     1681 atcctgggta ccggggaaag tgctgaaacc ccgatggtca gccagatgga ggatttcacg
     1741 tcgtcgcggg cgttccgggt cgccggaccg acggcgttca aggaggccgg gatcacccac
     1801 aaggacgtcg atcacctgat gatctacgac gccttcgcgc atctgccgat ctacggcctg
     1861 gaggacatcg ggttcctgcc gaagggcgag gccgccgcct tcatcgcggg acggaacacc
     1921 gcgccgggcg gttcgctgcc tttgaacacc aatggtggcg gactgtcgta tatgcactcc
     1981 ggcatgtatg ggatgtacgc gttgcaggaa agcgtgcggc aaatgcgcgg gaccgctccg
     2041 gcgcagatcg ccggggcgaa gatctcggtc tgccatggcg tcggcgggat gttcgccgcg
     2101 tccggcacga tcatcttcag caatcaggcg ccgtagttcc aatcacccgt catggtttcc
     2161 tggaaccatg acggctggct gcataaccat cgtattgggg accgattatg accgagagcc
     2221 tgttcacggg cctgaaagtc atcgactgcg cgagctggat cgcaggaccg gcggcagcga
     2281 cgatgatgtc ggatttcggc gccgacgtga tcaagatcga gccccccggc gccggcgatc
     2341 catggcgtgc ttcgaccccg ataccgggga aacctgtgga ctactactgg cagttgacgt
     2401 cgcgcaacaa gcgcagcctg gcactcgatc tgaagcatcc ggacgggcag gcggtgctgt
     2461 accggctggt cgcttcggcc gacgtgttca tcaccaatta tccgctgccg gtgcgcgacc
     2521 ggctgaaact ggcgccggcg gatctgctgc cgttgaaccc gcggctgatc tacgcgtcgt
     2581 tcacggcgta tggggaagcg ggagaggaag cggccaagac cgggttcgat tcgaccgcct
     2641 actgggctcg taccgggttg atggacatgg tgcgtgcgag ccccgatacc gtgccgtccc
     2701 gctcgatgcc cggcatgggc gacttcccca gtgctacggg tgtctatgca gccatcgtga
     2761 cggcgttgta tcgccgtgag aaaaccgggc aaggcggcgt ggtgcggtcg tcgttgttgc
     2821 agagcggtct ttgggccaac gggtgtgcgg tgcaaaccag gctgttcggg gaagatgtcg
     2881 gcctgcggcc ac
//
LOCUS       DASWQN010000579         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1243654, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000579 DASWQN010000000
VERSION     DASWQN010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243654"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(473..>2283)
                     /gene="recG"
                     /locus_tag="VGC82_09680"
     CDS             complement(473..>2283)
                     /gene="recG"
                     /locus_tag="VGC82_09680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY0423578.1"
                     /translation="WRIIVKDDTGIAELVFFKFTREAQMPPGAKLLVSGKLDMFNGRL
                     TIAHPDHLVPIDQPERIPLIEPVWPLTAGLWPRQVAAGLAQALTLLPSLPEWHDPALL
                     RREKWPPFEEALRTVQAPSAGEPDRRMRVRLAYDELLAGQVAQALVRGRVRARSGQPL
                     VGDSRLRDVALRRFGFSPTPSQANALREIDADLASPRRMLRLLQGDVGSGKTLVALLA
                     MLRAVEAGKQTALMAPTEVLAKQHHRTLTRLCPVPVALLTGSIKGKERARLLRGIKDG
                     TIQVVVGTHALFQDAVDYRDLALAVIDEQHRFGVNQRLTLGAKGEHTDVLIMTATPIP
                     RTLLLTQWGEMDVSRLTEKPAGRLPIRTTLHSLGTMPTVLEGIARKLDEGAQIYWVCP
                     LVAESEALDVAAAQERHADLKQRFGERVGLAHGQQTPEVRDKALAAFTAGTIRLLVAT
                     TVVEVGVDVPAASVMVIEHAERFGLAQLHQLRGRVGRGAEASFCLLLHEDWVNETARR
                     RLTLLRDTEDGFLIADEDYRLRGGGDALGTRQAGLPGYRLADPVDHEHLLHMANRDAA
                     VLLSRDPKLQTARGLAVRTLLRLFERLPAMRTLAAG"
BASE COUNT          375 a          751 c          777 g          380 t
ORIGIN      
        1 ccgaggttca ttggatgccg gtggcggcat ttctatgccg cgcgaagatg ggacggccta
       61 ccatgcttgg cgggctgaga ttgatcgcgg gccgagcggc cagacgccga atgcgattga
      121 attgttgcga tcaatgactt cgcggttaga cgcaatgcgt cggccggagt ggtttgacgg
      181 tgaccggaaa caggtactgc gtcgttgcaa aggaagtcgt gggtggccgc gccgagcagg
      241 ccatgacacc cgtgggtggc cccgccaagc ccggccatga cgcattgacg ccccaggcgg
      301 caaactggct cgcacctttt ccggtcagcg tgaacgcgtt ctacgagcca gcagggctta
      361 tttccgtggc ggtccgtggc gtcatcccgc ttctccgtga tgaaacctgc tccgcgatga
      421 aacctgcagg cttgataacc acggcaattc ggtttccagc ctgaacccgg tgctaaccag
      481 cggccagcgt ccgcatcgcc ggcaggcgct cgaacaatct cagcagcgtc cggactgcca
      541 ggccacgcgc ggtttgcagc ttgggatcgc gtgacagcag cacggcggcg tcgcggtttg
      601 ccatgtgcag caggtgctcg tgatccaccg gatcggccaa ccggtatccc ggcaatccgg
      661 cctgcctggt gccaagtgca tcgccgccgc cgcgcagccg gtaatcttcg tcggcgatga
      721 ggaacccgtc ctcggtgtcg cgcagcaggg tcagccggcg ccgagcggtt tcgttgaccc
      781 agtcttcgtg gagcaacaga cagaagctgg cttcggcgcc gcggccgacg cgcccgcgca
      841 actggtgaag ctgggcgagg ccgaagcgct ccgcgtgctc gatcaccatc accgaagcgg
      901 ctggcacatc cacgcctacc tccacgaccg tggtggcgac cagcaaccgg atcgtgccgg
      961 cggtgaaggc ggcaagagcc ttatcgcgca cctccggcgt ttgctggccg tgcgcgaggc
     1021 cgacccgctc gccgaagcgc tgcttcaggt cggcatgacg ctcctgtgcg gcggcgacgt
     1081 ccaaggcctc gctttcggcg accagcgggc agacccagta gatctgggcg ccttcgtcca
     1141 gcttgcgggc gataccttcc aggaccgtcg gcatggtgcc cagcgagtgc agggtggtgc
     1201 ggatcggcag ccggccggcg ggtttctcgg tcaggcggct gacgtccatt tccccccact
     1261 gcgtcagcag caaggtccgc ggaatcggcg tcgcggtcat gatcagcacg tcggtgtgct
     1321 cgcccttggc gcccagcgtc aggcgctgat tgacgccgaa acggtgctgc tcgtcgatca
     1381 ccgccaaggc aaggtcgcgg taatcgactg cgtcctgaaa cagcgcatgg gtgccaacga
     1441 cgacctggat cgtcccgtcc ttgatgccgc gcagcagacg ggcccgctcc ttgcccttga
     1501 tcgaaccggt cagcagggcg accggcaccg ggcacagcct ggtcaaggtg cggtggtgct
     1561 gcttggccag cacctcggtc ggcgccatca gcgcggtttg cttgccggct tccaccgcgc
     1621 gcagcatggc cagcaacgcg accagcgtct tgccggagcc gacatcgccc tgcagcagcc
     1681 ggagcatccg gcgcggggaa gcaaggtcgg cgtcgatctc tcgcagcgca tttgcctgcg
     1741 atggcgtggg cgagaaaccg aaccgccgca gggcgacatc gcgcagccgc gaatcgccga
     1801 ccagcggctg gcccgaccgc gcgcgcaccc ggccgcggac cagcgcctgg gcaacctgtc
     1861 cggcgaggag ttcgtcgtac gccaggcgga cccgcatccg gcggtcgggt tccccggcgg
     1921 acggggcctg gaccgttcgc agggcttctt cgaacggcgg ccatttttcg cgtctcagca
     1981 acgcaggatc gtgccattcc ggaagtgacg gcaacaacgt cagtgcctgg gccaatccgg
     2041 cggcgacctg gcgcggccaa agcccggcgg tcagcggcca aaccggctcg atcagcggaa
     2101 tccgctcagg ctggtcgatt gggacgagat ggtcggggtg ggcgatcgtc agccggccgt
     2161 tgaacatatc aagcttgccg gagaccagca gtttcgcgcc cggcggcatc tgggcttcgc
     2221 gggtgaattt gaagaatacc agttcggcga tgccggtgtc gtccttgacg atgatccgcc
     2281 agg
//
LOCUS       DASWQN010000580         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1111092, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000580 DASWQN010000000
VERSION     DASWQN010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111092"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..668
                     /locus_tag="VGC82_09685"
     CDS             45..668
                     /locus_tag="VGC82_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423579.1"
                     /translation="MEDPREGKLIEAFSPQYRERTIMLSVANFFQTIGFYGFANWVPT
                     LLIAKGIHVSQTLEYTFIIAFAYPLFPLISSLFADKMERKWQVCVSCLGIAVFGILFS
                     TQSAALPLIIIGTFQTMMNAWLSFSAHNYMAELFPTRMRASAVGFVYSWSRFSTIFTG
                     FIIAWLLGQFGVPGVFLFVAGAMAVVVLSVGIFGPRTNLLALEAISH"
     gene            complement(819..1376)
                     /locus_tag="VGC82_09690"
     CDS             complement(819..1376)
                     /locus_tag="VGC82_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423580.1"
                     /translation="MLLAFSFIFFAQYIYWGVFTWTPTFLVSVKHLTFVRGLGFVLSQ
                     QIGSLLGFLVFAALVDRLGRRPTFIAYLVIGAIAVGVLVLEANPEVLLVTMFVTGFGI
                     TGIFAGMGPFTAEMVSNSSARGLAMGIAYNGGRIGGLIAPVLIGALATSEQGFVIGMA
                     TTIAAFILAIGVILICPETRGTEIN"
     gene            complement(1280..2095)
                     /locus_tag="VGC82_09695"
     CDS             complement(1280..2095)
                     /locus_tag="VGC82_09695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423581.1"
                     /translation="MADNWAPPVGTTGTVAATSWYREITPQSWRVLGFAGLGWMFDVY
                     DSFVLSLTLPALIAAFALSNAEAGTIGSILAAGLIVGGITLGWVADRIGRVRTLYLSI
                     LVYSLFTCLTAFAPSATWVAVLRFFGGLGMGGTWTAGAALVAETWGAKHRGKGGALMQ
                     MGLPIGSMLAIGVTALVTSLMGGLNNGGWRVIYGLGCLPALILLPVALHTPSRRYGSP
                     ARHRPPSERRSLICSEAAMHAACCWPSASSSLRNISTGACSPGHQPFWSASSI"
BASE COUNT          459 a          669 c          660 g          389 t
ORIGIN      
        1 ccgctgaatc tggcgcgccc ctgcccccgc ctggaccgcc tgcgatggaa gacccacgtg
       61 aaggcaagct gattgaagcc ttcagtccgc aataccggga gcggacgatc atgctgtcgg
      121 tggcgaattt tttccaaacg atcggctttt acggcttcgc gaactgggtg cccacgctgc
      181 tgatcgccaa gggcatccat gtgtcgcaga cgctggaata caccttcatt atcgccttcg
      241 cctatccgtt gttcccgctg atcagctcgt tgttcgccga caaaatggag cggaaatggc
      301 aggtctgcgt ctcttgcctc gggatcgctg tattcggcat cctgttttcg acgcagagcg
      361 cggcgctgcc gttgatcatc atcggcacgt tccagacgat gatgaacgct tggctgtcgt
      421 tctcagctca caactacatg gcggagctgt tccctacccg catgcgcgcc agcgcggtcg
      481 ggttcgtgta ttcctggagc cggttcagca cgatcttcac cggcttcatc attgcctggc
      541 tgctggggca attcggcgtg cccggcgtgt ttctgttcgt cgccggcgcg atggcggttg
      601 tcgtattgtc agtagggatt ttcggtccac gcaccaacct gctggcgttg gaggcgattt
      661 cacactgatg gttaggatga ccaggtcatg acgaacatac gtgacggtaa ggccaacaga
      721 attgtgtcaa atgacgaaat agatgactgc cgcgggcgaa cctgggcaac gcaaggatgg
      781 caagacatcg gaacgcgacg acgttgctgg atcaagcctt aattgatctc ggttccccgc
      841 gtttccggac agatcaggat gacaccaatc gccaggatga aggcggcgat cgtcgtcgcc
      901 atgccgatga caaacccctg ttcgctggtc gctagtgcgc cgatcaggac gggtgcgatt
      961 agcccgccga tacggccacc gttataggca atgcccatgg cgagaccacg cgcgctgcta
     1021 ttcgacacca tttcagcggt gaacggcccc atgccggcaa aaatgccggt gatgccgaac
     1081 ccggtgacga acatcgtgac cagcagcacc tccgggttgg cctccagcac caacacaccg
     1141 acagcgatcg cgccgatgac aagataagcg ataaaggttg gccggcggcc cagtcggtcg
     1201 accagcgcgg cgaagaccag aaagccgagt agggaaccga tctgctggga cagcacaaag
     1261 ccgaggccgc gtacgaaagt cagatgcttg acgctgacca gaaaggttgg tgtccaggtg
     1321 aacacgcccc agtagatata ttgcgcaaag aagatgaagc tgaaggccag cagcatgccg
     1381 cgtgcattgc ggcctcggaa cagatcagcg accgacgttc gctcggcggt cgatgccgcg
     1441 cggggctgcc ataccggcga ctcggcgtat gcagcgcgac cggcagcagg atcagcgctg
     1501 gcagacaccc gagaccgtag atcacgcgcc agccgccatt gttcagcccg cccatcaggc
     1561 tggtgactag ggcggtgacg ccgatcgcca gcatcgagcc gatcggcagg cccatctgca
     1621 tcagggcgcc gcctttgccc cgatgcttcg ccccccacgt ctccgcgacc agcgcagcac
     1681 ccgcggtcca tgtaccaccc atacccaagc caccaaaaaa gcgcagcacg gcgacccaag
     1741 tggccgaagg tgcgaacgcg gtgaggcagg tgaagagcga atagacgagg atgctgagat
     1801 acagcgtgcg gacccgcccg atgcggtcgg cgacccagcc cagggtgatg ccaccgacaa
     1861 tcagcccagc ggcgaggatg ctgccgatgg tgccggcttc cgcgttggac agcgcaaaag
     1921 ccgcgatcag ggcgggaagg gtgagcgaaa ggacaaagga atcgtagacg tcgaacatcc
     1981 agcccaaacc ggcaaagccg agcacgcgcc agctctgtgg cgtaatctcg cgataccaag
     2041 aggttgcagc cacagtgccc gtggtaccga ctggaggtgc ccaattgtct gccatgtgcc
     2101 cgtttccctc cgtctcgcca tctgccacgg cttgttctta ctctggacgc ctacgctgaa
     2161 ttccccaggg gaatggc
//
LOCUS       DASWQN010000581         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_979609, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000581 DASWQN010000000
VERSION     DASWQN010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979609"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..845
                     /locus_tag="VGC82_09700"
     CDS             117..845
                     /locus_tag="VGC82_09700"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423582.1"
                     /translation="MKGWRTRIGFLLPPGNPTVEPEMAELTPPGVSLHFTRMHAEGPA
                     GTHTGQEQRNQSQVESIPACVRLIAMVSPSVIVMAHTATSYMLGEAREAALVAEMEAV
                     GKARFITAFGSVMAAFNALGVRRVAYATPYSAEMTAQGERHLEKCGISVVSSGHLANV
                     RNIYEENSERAYAIGREVDHPDADAIFLSGVGMPTLDALRPLEEDTGKPVISAASAMM
                     WHALRTAGVNHGFDGYGKLLSGKF"
     gene            complement(951..1874)
                     /locus_tag="VGC82_09705"
     CDS             complement(951..1874)
                     /locus_tag="VGC82_09705"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0423583.1"
                     /translation="MDNTGNPITYVFEAWRGLLADPGKQLVFAALLLLLAAYRGWRSS
                     WPRRAVDTVESVKATLAIININLVLTPVVWLMSEQLKTLYALLGIPSVPGSVWAGLPL
                     WLLSLIAILAQDFANYWNHRAMHWKWLWPVHAIHHSDPVVNGLTTYRIHILEALVMWG
                     SYTILLTWLGMPADAIGIGALFLTFHNIYVHINVDWGHGPLRLLIASPRFHRWHHADV
                     PEAHGKNLANVFPFFDWMFGTYRVPGPCDAPVGAAGIPRNDVVKLTLWPLLEWARLAT
                     LSVEAMRARIAGWLARGAGLGKTMPLDIKAD"
     gene            complement(2051..2266)
                     /locus_tag="VGC82_09710"
     CDS             complement(2051..2266)
                     /locus_tag="VGC82_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423584.1"
                     /translation="MIAVTLALWFGLSGSNLAIVTACSAVPASSSAYVLARQMGGDAP
                     LLAQIITLQTILAAITMPVAIALAAWF"
BASE COUNT          443 a          683 c          766 g          411 t
ORIGIN      
        1 catctttctg ctcttcttat cgggaccgta cgtctaggcg ttgatccctg ctatctgcgt
       61 cgggtgtaat tgccgtggcg ttggtgtctc aaggcaagca cgaaggggac agttctatga
      121 agggatggcg tacacggatc gggtttctgc tgccgcccgg caatccgacg gtcgagcccg
      181 aaatggccga actgacgccg cccggcgtat cgctgcactt cacgcggatg catgccgaag
      241 ggccggcagg cacgcatacc gggcaggagc agcggaatca gagccaggtc gaaagcattc
      301 cggcctgtgt gcggctgatt gcgatggtct ctcccagcgt gatcgtgatg gcgcatacgg
      361 cgacgagtta tatgctcggc gaagcgcggg aagccgcgct ggtcgcggag atggaagccg
      421 tcggcaaggc ccggttcatc accgccttcg gcagcgtgat ggcggctttc aacgcacttg
      481 gcgtacgacg cgtggcttac gccacgcctt actccgcgga gatgaccgcg cagggagagc
      541 ggcatctcga gaaatgcggc atatcggtcg tgagttccgg acatctggcc aacgtccgca
      601 acatctacga ggaaaattcc gagcgcgctt atgccatcgg ccgggaggtc gatcatcccg
      661 atgccgacgc aattttcctg agcggtgtcg gcatgccgac gctcgatgcg cttcgccccc
      721 tggaagagga taccggcaag cccgtgatct cggcggcgtc tgccatgatg tggcacgcct
      781 tgcgcacggc tggcgtcaac cacggatttg acggctatgg aaaactgctg agcgggaaat
      841 tctgaaaatg aagccggtgg tgtgactggc caggcgcagc atggcggtca tgcccgctac
      901 tggtttggag cggcgcggtg aaccgcggcg ttaccaccat ctctgccatt ctagtcagcc
      961 ttgatgtcga gcggcatggt cttgccgagg ccggcgccgc gcgccagcca accggcgatc
     1021 cgggcgcgca tggcctcgac cgacagggtc gccagccgtg cccattccag cagtggccac
     1081 aatgtcagct tcacgacatc gttgcgcggt atgccggcgg cgcctaccgg tgcgtcgcat
     1141 ggtcccggga cgcgataggt gccaaacatc cagtcgaaga acggaaagac gttggcgagg
     1201 ttcttgccgt gggcctccgg cacatcggca tggtgccagc ggtgaaagcg cggggaggcg
     1261 atcaacagcc gcagcgggcc gtggccccag tcgacattga tatggacgta gatgttgtgg
     1321 aacgtgagga atagcgcacc gataccgatc gcgtcggcgg gcataccgag ccacgttagc
     1381 aagatggtat aggagcccca catcaccagc gcttccagga tgtggatgcg atatgtcgtc
     1441 aagccgttga cgactggatc ggaatgatgg atcgcgtgca ccggccacag ccatttccaa
     1501 tgcatggcgc gatggttcca gtaattggcg aaatcctgag ccaggatggc gatgaggctg
     1561 agcagccaga gcggcaatcc cgcccagacg gagcccggaa cactggggat tccgagcaat
     1621 gcatagagcg tcttcaactg ctccgacatc agccacacga cgggtgtcag cacgaggttg
     1681 atgttgatga tggcgagcgt tgccttcacg ctttcgaccg tatcgaccgc gcggcgcggc
     1741 caggaagatc gccaacccct gtaggcggcc agcaacagca gcagggcggc gaagaccagt
     1801 tgcttgccgg gatctgccaa caggccacgc caggcctcga acacgtacgt gatgggattg
     1861 cctgtattgt ccacgttgcc tgcgctggtc tggatgcaaa gccggcgcgg acccattgcg
     1921 aagccagccc gccgcgcggt tttagcggga tttgccttaa ggcagggtga atcgaaccct
     1981 ccggttccgg ttacagttcc gccgcctgcg gtatggaatg gggtggttac gcgccttgat
     2041 gcataggcaa ctagaaccag gccgccagcg cgatcgccac cggcatcgtg atcgccgcca
     2101 ggatcgtctg cagcgtgatg atctgcgcca gcagtggcgc gtcgccgccc atctggcggg
     2161 ccagcacata ggcgctggag gaggccggca ccgccgaaca ggcggtgacg atggcgaggt
     2221 tggagcctga cagcccgaac cacagcgcca gcgtcacggc gatcacgatg ccggtggcga
     2281 tcgcgctggc ggcctggttc tag
//
LOCUS       DASWQN010000582        10525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1639888, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000582 DASWQN010000000
VERSION     DASWQN010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10525
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639888"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VGC82_09715"
     CDS             <1..500
                     /locus_tag="VGC82_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY0423585.1"
                     /translation="SPAASSPAASSPAASSGSHWCDGDCQPVLREALVDAMRELAARY
                     GDDPATWRWGEVHQAIFAHPILRNVPLLGSLSTISVPSPGDDDTIDRGGTDRAFRSVH
                     GAAYRGVYDLADLDRSLFMIAPGQSGNLFDVHARDFIHRWRDGATITLGPTAATVIGT
                     VRLVP"
     gene            497..994
                     /locus_tag="VGC82_09720"
     CDS             497..994
                     /locus_tag="VGC82_09720"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0423586.1"
                     /translation="MSFGNPYLDDDFLTGGVMSRRCIAWVLDFLVLAVLIWVLWWILV
                     MFGFLTFGLGFGAMSILPFVPFCYHFLSLLSAASATPGQRMMGLTVRRNYDLGPPDGL
                     QALVSVLLFYLTMATSGLLLVVALFTTRHRALHDLVSGLVVVRVQALTSGDTGWNMQG
                     GAPAP"
     gene            1033..1815
                     /locus_tag="VGC82_09725"
     CDS             1033..1815
                     /locus_tag="VGC82_09725"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEY0423587.1"
                     /translation="MTMYKPPRRPQFFYTTAPLPCPYLAGRTERKVVTEIVGPEAEAL
                     HDRLSRAGFRRSHNIAYAPVCPSCQACVPIRIPVETFQPDRTLRKVAKSNAGLEGYRV
                     AARATAEQFQLFQRYQQVRHGDGDMAGMSFYDYRAMVEDTPIETFIIEFRDDQDRLVC
                     ACLADKLGDGLSAVYSYFAPGLEKRSLGTYAVLWLIERARALGLPYVYLGYWVPESRK
                     MAYKARFKPSEVLISGTWRLLTEGEIAGTDAAEAAMVTESAG"
     gene            1832..2503
                     /locus_tag="VGC82_09730"
     CDS             1832..2503
                     /locus_tag="VGC82_09730"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0423588.1"
                     /translation="MTQPSAHERPADEGTADRLWLVWAREIHAIAQTGLAFSKDPYDL
                     ERYAALRRLSARIMAEHTGAGLDRIEALFDGETGYATPKVGVRAAVFDPYGRILMVRE
                     TADGNRWTLPGGWADVNQTPAQSVVREVFEESGYRVHAVKLAAVWDRARQAQPPTVFS
                     VVRTFFVCALQGGEPRTSLETSEIGWFAESDIPEDLSLRRTLPHHISRMFAHHRDPGL
                     PTDFD"
     gene            complement(2798..3367)
                     /locus_tag="VGC82_09735"
     CDS             complement(2798..3367)
                     /locus_tag="VGC82_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0423589.1"
                     /translation="MSAANEMPAAMTAAEFLAWNPQGSDRWELIDGIPRAMAPAAPRH
                     GAIQSEVSRLIGNCLAESRPACRVIIEPGIQPKVRADLNVRVPDLAITCAASDPGDRL
                     LREPLVVIEILSPSNKAETWANVWSYVTIPSVREILVLHTADIRADLLYRDADGIWPD
                     NPLTLMPGDTVTLQSIDFTAPIAAFYRTA"
     gene            3619..4527
                     /locus_tag="VGC82_09740"
     CDS             3619..4527
                     /locus_tag="VGC82_09740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0423590.1"
                     /translation="MILPRQTQTTDAVAAHYDELDSFYREIWGEHVHHGYWATGRETP
                     AEAAVSLLDLLADRLRLEPGLRVCDIGCGYGAAARYLAETHALDVVGVTISAAQARRA
                     QIQRAQAQCAEAQCARARRPQPPAPTAGSVTVWLQDWLANDFAPASFDRAYAVESSEH
                     MPDKQGFFHEAFRTLKPNGLLAVCAWLSRTEPAAWEIRHLLEPICREGRLPSMGDETD
                     YRRLGERAGFRVVQVEDLSRQVSRTWWICIRRGLLKLATRPRYVRFLLDKTASNRIFA
                     VTLFRIMIAYRTRSMRYCVLLFRREA"
     gene            complement(4564..5808)
                     /locus_tag="VGC82_09745"
     CDS             complement(4564..5808)
                     /locus_tag="VGC82_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEY0423591.1"
                     /translation="MLHAALGLASLLLLAFLCSEDRRRIPWRTVAGGVALQIAFALLL
                     IGVPGADRGMFWLSDAANAVHAATLVGTQFVFGYLGGGAPAFAEVHPGASFILAFQAL
                     PLVLTISALASLLFHWGVLQRITAGFAWLLRRSLGIGGPLALGAAVHVFVGMVEAPLL
                     VRPYLMRMQRGELFALMTCGMAGVAGTVMIIYASFIAGFVPNSLGHLLIASIVSTPAG
                     LAVAALMVPFDHSDRPEGQLVIENPPVSAMDALVKGTMDGVPILAAIIATLLVTVAIV
                     TLVNMTLGLLPNWDGGAVTLQRLFALPFRPVMWLIGVPWAETATASMLMATKTVLNEF
                     VAYLDFSRMPADTFSPRSRMILTYALCGFANFGSLGIVIGGLGAMVPARRAEIVGLGL
                     RTILSGTLATCMSGAVAGALTG"
     gene            complement(5814..7472)
                     /locus_tag="VGC82_09750"
     CDS             complement(5814..7472)
                     /locus_tag="VGC82_09750"
                     /inference="COORDINATES: protein motif:HMM:NF005470.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0423592.1"
                     /translation="MASDYTVGDLIAEFLSACGVTTVFGIASVHNIPMLDAIGRRNTI
                     RFMMARGELGGAHMADGYARVAEGLGVIISSTGPGAANTIGGLIEARFAGSPVLHITG
                     QTSTRYADRGMGSVHDPVDQLGMMASVCKSAYRIRSAQNALGLLTKAAMEALSAPMGP
                     VSVEVPIDLQRAKIERPAMLDNFVLPLPAPRVPTDTELDELASRVIAAKRPMLWLGNG
                     AKKAGSAASKLLQMGFGMVTSFNGRATVPEEHPRNLGGLTGIGMPIIADFYKTVDLCL
                     VVGCRLRGHETNDFQVQLPDNIIQVDADPAANGRTYASQYFVCGDANATLEGLVSRIE
                     GKLKVAPGYAEEFADLKRNAQAVFIETLGAYGTFSAQLRKVMPGDAVWARDVTQNNTT
                     WGNRVFPLNAPHQNVYPVGAGIGQGLCLGIGAAAAAAGRKTVVMTGDGGFFLNVGELW
                     TAVQEDLDMTVIVMNDRGYGVIKRIQDATAGGRRFYADLESPDIEKLAGLSDIPYFRC
                     SRADTFGDTVGKALAIKGLTMVEVDMTAVGDFPPYYPFNQRQAG"
     gene            complement(7525..8820)
                     /locus_tag="VGC82_09755"
     CDS             complement(7525..8820)
                     /locus_tag="VGC82_09755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423593.1"
                     /translation="MRKVAWRLVPFLSFAYLINALDRFNVSIAALTMNKELGLSATTY
                     GLAAGAFFWSYVLFQLPASAILTRIGARRWLGVTMVVWGACSAGTAFVTDATSFVTMR
                     FLLGIAESGFFPGVAWFMTRWFPSRHRGGAMGIFYAFGASASVIGGPVSGNLLLLHGW
                     HGLSGWQWLFLIEGIPACVLAGLCPILMRDRPEDAAWLTRSEQSWLRERLDAESDQVS
                     TQKLSLMQSIVSPMMLMLILGYLLIGFGVYSKNFFLPLMIKTMGYSDQTVGYLSSLPN
                     LFGCLGMILFSRNSDRTGERIWHVAVPCLLAAVGLLGAGLTLSINPVVAMACFCLAGF
                     GISSSLPCFWNLPTAWLGPAAAAGGIAVINSIGNISGYVAPQMVGLLRDATGSYEVPM
                     LVASLFMLAAAICIVLSPRAAARPVVAQPVLQRPVPPRR"
     gene            complement(8996..10087)
                     /locus_tag="VGC82_09760"
     CDS             complement(8996..10087)
                     /locus_tag="VGC82_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEY0423594.1"
                     /translation="MKAAVFHAAHQPLTIEDVQIEKPKRREVLLRTAYAGLCHSDLHF
                     MEGLYPFPAPAVLGHEGAAVVEAVGEDVTYVKPGDHVITCLSVFCGECAQCTTGHTNL
                     CENTEVKLLPGAARRLKWKNAVLNQAFNLSAFAEQMLVHEHAVVKINKDIPLDRAALI
                     GCGVMTGVGAVFNAAKVEPGSTVAVVGCGGIGLSAVNGAALAGAARIIAIDTNASKLE
                     KAREMGATDTINASNVDPVAAVKDLTDGQGVPYSFEAIGLKITAEQCFAMLRPGGVAT
                     IIGMIPFGTKIELHGADFLRDRKIQGTSMGGNRFRVDMPRLLELWRQGRLKLDTLLSG
                     EIKLDQINEGFAELKSGAPVRNLINFGVV"
     gene            complement(10138..>10525)
                     /locus_tag="VGC82_09765"
     CDS             complement(10138..>10525)
                     /locus_tag="VGC82_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0423595.1"
                     /translation="DVTRNGLDLAKRAAADWDKPLDLNVLVPILILHDVDKPLMYLRE
                     GAGKVVYSKLYHELQHGVAGAMLLKELGFDHTVVSTVALHAGNSPYHGRNHEAYVLHY
                     ADYFATDHAIMLEGPDAEPYYQKHWR"
BASE COUNT         1962 a         3429 c         3228 g         1906 t
ORIGIN      
        1 cttcccctgc ggcatcttcc cctgcggcat cttcccctgc ggcatcctcc gggtcgcatt
       61 ggtgcgacgg cgactgccag ccggtcctgc gggaagcgct ggtcgatgcg atgcgcgagc
      121 tggctgcccg ttacggcgac gatccggcga catggcgatg gggggaggtg catcaggcca
      181 tcttcgcaca tccgatcctg cgcaatgttc cgctgctcgg ttcgctctcg acgatcagcg
      241 tgcctagtcc gggcgatgac gataccatcg atcgcggcgg gacggaccga gcgttccggt
      301 cggttcatgg agcggcttac cgcggggttt atgacctggc tgacctcgac cgcagcctgt
      361 tcatgattgc gccgggacag tcgggcaatt tgtttgacgt ccatgcaaga gatttcatcc
      421 accgttggcg cgacggtgct acgataacgc tggggccgac ggcggccacc gtaatcggga
      481 cggttcgact ggtaccatga gcttcggcaa cccctatctg gacgatgatt tcctcaccgg
      541 cggcgtcatg tcgcgccgct gtatcgcttg ggtgctggat ttcctggtgc ttgcagtact
      601 gatttgggtg ctgtggtgga tcctggtcat gttcgggttc ctgaccttcg gcctgggttt
      661 tggcgccatg agcatcctgc cgttcgtgcc gttctgttac cattttctgt cgctgctttc
      721 ggcggccagt gcgaccccgg gccagcgtat gatgggcctg acggtgcggc ggaattacga
      781 tctcgggcca ccggacgggc tgcaggcact ggtctccgtc ctgctgttct acctgacgat
      841 ggcgacgtct ggcctgctgc tggtggtcgc gctgttcacc acccgccacc gtgcgctgca
      901 cgacctcgtg agtggtctgg tcgtggtgcg ggttcaagca ttgaccagcg gggacacagg
      961 ctggaacatg caaggcggag cccctgcccc atgacccagc gcggataacc cgatcctgat
     1021 gacccgatcc tgatgacaat gtataagccg cctcgtcggc cgcagttctt ctacacgaca
     1081 gcgcctttgc cttgcccgta tctggcggga cgcaccgaac gcaaagtggt gacggagatc
     1141 gttggtcccg aggccgaggc gctgcacgac aggctatccc gcgccgggtt ccgccgcagc
     1201 cacaacatcg cctatgcgcc ggtctgcccg tcctgccagg cctgcgtgcc gatccgcatt
     1261 ccggtggaaa cgttccagcc ggaccgaacg ctgcgaaagg tcgccaagtc caacgcaggg
     1321 ctggagggat atcgcgtggc tgcccgagcc acggccgaac agttccaatt gttccagcgg
     1381 tatcagcagg tgcgccacgg cgacggcgac atggccggca tgagtttcta cgactatcgg
     1441 gcgatggttg aggatacccc gatcgagacc ttcattatcg agttccgcga cgatcaggac
     1501 cggctggtat gcgcatgtct cgcggataaa ctgggcgacg ggttgtcggc cgtttacagc
     1561 tacttcgcgc ccggcctgga aaagcgttcg ctcgggacat acgcggtgct ttggctgatc
     1621 gaacgcgccc gggctttggg attgccctat gtttacctcg gctattgggt gcccgaaagc
     1681 cggaagatgg cctacaaggc gaggttcaag ccaagcgaag tgctgatcag cgggacgtgg
     1741 cggttgctga cggaggggga gatcgccggc acggacgcgg ccgaggccgc gatggtgacg
     1801 gaatccgccg gttgaggcga agcggccgga cgtgactcag ccctcggcac acgagcgccc
     1861 ggcagacgaa ggcacggccg acagactctg gttggtctgg gcgcgtgaaa tccacgccat
     1921 cgcacagacc ggcctggcct tcagcaagga tccttacgat ctggaacgct atgccgcgtt
     1981 gcgacgtctc tccgcccgga tcatggcgga gcacaccggc gccggcctgg accgcatcga
     2041 ggctttgttc gatggcgaaa ccggttacgc aacgcccaag gtcggggtcc gcgcggcggt
     2101 gttcgatcca tatggccgca tcctgatggt gcgcgagacc gccgatggga accgctggac
     2161 gctgcccggc ggatgggcgg acgtaaacca gactccggcc cagtcggtcg tgcgggaagt
     2221 cttcgaggag tccgggtatc gggttcacgc agtgaaactc gccgccgtct gggaccgagc
     2281 gcgtcaggcc cagccgccaa cggtcttttc ggtggtgcgg acgttttttg tctgtgcgct
     2341 gcagggtggg gagcctcgca ccagcctgga aacgagtgaa ataggctggt tcgctgaatc
     2401 cgatattccc gaggatctgt cgttgcgccg gacgttgccg catcacatca gccggatgtt
     2461 cgcgcaccac cgggatccgg ggctgccgac ggatttcgac tgaatcgacg cgtcgcgcgt
     2521 gcagatcaca ttgtccattg ccaccaaccg actggtgaca ccgacaaccg gctcgttttc
     2581 aaggacatcg accgccgcca tgccggggcc gcggccaacc gcgatcgacc tggtgctcgg
     2641 tctcaccaaa aagcgaactg gtctccgatc ccgtatttgt ttctttttag aaccaattac
     2701 ttgatctcgc gggtcgccac caaacgcgtg ccgttgtggg agcttccggc tgagggagca
     2761 acggcccgcc ggcggtttgc gcccgagctg gcagacgtca agccgtgcgg tagaacgcgg
     2821 cgatcggcgc ggtaaaatca atgctctgca atgtcacggt gtcgcccggc atcagtgtca
     2881 gcgggttgtc cggccatatg ccatctgcgt cccgatacag caggtcggcg cgaatgtcgg
     2941 cggtgtgcaa cacgagaatt tcccgcacgc tggggatcgt cacgtagctc caaacgtttg
     3001 cccaggtttc cgccttgttc gagggcgaca ggatctcgat cacgacaagc ggttcgcgca
     3061 gcagccggtc gccggggtcc gatgcggcgc aggtgatcgc gaggtccggc acccgcacgt
     3121 taaggtccgc gcgcaccttg ggctggatgc cgggttcgat gatgacacgg cacgccgggc
     3181 gcgattcggc caggcagttg ccgatcaacc ggctgacttc gctttgaata gccccatgtc
     3241 gcggggccgc cggcgccatt gcgcggggga tgccgtcgat cagttcccac cggtcgctgc
     3301 cctgcgggtt ccaggccaga aattcagccg ccgtcatcgc ggcgggcatt tcgttcgcgg
     3361 cgctcatgtt tgtcctctat cggaatatgc gctcctccgg acctggccgg tcaagcggca
     3421 atcccgctgc tgcatcctga acccgcatga tggtcgtccg atggtgcgaa ccgccaagcc
     3481 gggaccgata cgctcgatcg ggaaaaacca tcattggcgg aaatacgagg ctgtaggcag
     3541 gatcgttcgg acggctggac acgacgctcc ctgccatcaa atatccgcaa cgataaccat
     3601 ggtgcgaaag gcaaatgcgt gatcctcccc aggcagacgc agacgacaga tgccgtcgcg
     3661 gcgcattacg acgaactcga cagcttctac cgggagatct ggggcgagca tgtgcaccat
     3721 ggatactggg ccaccgggcg agaaacgcct gccgaagctg ccgtgtcgtt gctcgacctg
     3781 ctggcggacc ggttgcggct ggaacccggt ctgcgtgtct gcgacatcgg ctgcggttac
     3841 ggcgcagcgg cccggtacct ggccgaaacg catgcgctgg acgtcgtcgg cgtgaccatc
     3901 tccgctgccc aggcccgacg cgcccaaatc caacgcgccc aagcccaatg cgccgaggcc
     3961 caatgcgccc gagcccggcg cccccagccg cctgcgccaa cagcgggtag cgtaactgtc
     4021 tggctgcagg attggctcgc caacgacttt gcgccggcaa gcttcgatcg tgcctatgcg
     4081 gtggaaagct ctgaacacat gccggacaag caagggttct tccacgaggc tttccggacc
     4141 ctgaagccga acgggctgct cgcggtctgc gcctggctta gccgaaccga accagcggcc
     4201 tgggaaattc gccatctgct cgaaccgatc tgccgggaag ggcgcctgcc cagcatgggc
     4261 gacgagacgg actatcgccg gcttggggaa cgagcaggat ttcgggtggt tcaggtcgag
     4321 gacctcagtc gccaggtaag ccgaacctgg tggatctgca tccggcgggg gctgctcaag
     4381 cttgccaccc ggccgcggta tgttcgcttc ctgctggaca agacggcgag caaccgcatc
     4441 ttcgcggtca ccttgtttcg catcatgatc gcctatcgaa cacgatcgat gcgctattgc
     4501 gtccttctgt tcaggcggga agcctgactg tccggcgccc gttcggctga aaatgcttcc
     4561 gcattatccc gtcaaggcgc ctgcgaccgc tccgctcatg cacgtcgcca gcgtcccgga
     4621 gaggatcgtc cgtagcccca gcccgacaat ctcggctcgt ctggccggca ccatcgcccc
     4681 aaggccgccg atcacgatcc ccaggctgcc aaagttcgcg aacccgcaca gcgcatacgt
     4741 caaaatcatt cggctgcgtg gcgagaacgt atccgccggc atccgcgaga aatccaggta
     4801 agcaacgaat tcgttcagca ccgtttttgt cgccatcagc atggacgccg tagcggtctc
     4861 ggcccagggc actccgatca gccacatcac cggacggaac ggcagcgcga acagccgttg
     4921 caacgtgacc gcgccgccgt cccagttcgg cagcagtccc agcgtcatgt tcaccagtgt
     4981 gacgattgcc acggtcacca gcaatgttgc gataatcgca gccaggatcg gcaccccgtc
     5041 catcgtcccc tttaccaggg catccatggc actcaccggg gggttctcga tcaccaactg
     5101 gccctcgggc cgatccgaat gatcgaacgg caccatcagc gccgccaccg ccagccctgc
     5161 cggcgtgctg acgatggacg cgatcagcag atgtcccaac gaattgggaa caaagccggc
     5221 gatgaacgag gcatagatga tcatcaccgt tccggcgaca ccggccattc cgcaggtcat
     5281 cagagcgaac aactcacccc gctgcatccg catcagatat ggccgtacca gcaatggcgc
     5341 ttccaccatg cccacaaaaa catgcaccgc cgcgcccagc gccagtgggc cgccgatccc
     5401 cagggaccgc cgcagcagcc acgcgaatcc tgccgtgatc ctttggagca cgccccagtg
     5461 aaacagcagc gacgccaggg cgctgatcgt cagcaccaat ggcagcgcct ggaacgccag
     5521 gatgaaactg gcgccgggat gaacctccgc aaatgcaggg gcgccacctc caaggtagcc
     5581 gaaaacgaac tgggtgccca ccagcgtcgc cgcgtgcacg gcattcgctg catcgctcag
     5641 ccagaacatc ccccggtcgg cccccggcac gccaatcaac agcagagcga acgcgatctg
     5701 cagtgccacg ccgccggcga cggtgcgcca tgggatccgg cgccgatcct cgctgcacag
     5761 gaacgccagc agcaacaggg atgccagtcc cagagctgcg tgaagcaccg agatcagccg
     5821 gcttgccttt ggttgaatgg ataatatggc gggaaatcgc ccaccgccgt catatcgact
     5881 tcgaccatcg tcagcccctt gatcgccagt gccttgccga ctgtatcgcc gaacgtgtcg
     5941 gcgcgcgagc agcggaaata cggaatgtcc gacagcccgg ccagcttctc gatatccggg
     6001 ctttccagat cggcgtagaa acgtcgtccc ccggcggtgg catcctggat gcgcttgatc
     6061 acgccgtatc cccgatcgtt catcacgatc acagtcatat cgaggtcctc ctgcaccgcg
     6121 gtccaaagct cgcctacgtt caggaagaaa ccgccatcgc ctgtcatgac aaccgtcttg
     6181 cgcccagccg ctgcggcagc ggcgccaatg ccgaggcaca ggccttggcc gatccccgcg
     6241 cccaccggat agacattctg atgcggggca ttcagcggga aaacccgatt tccccatgtt
     6301 gtattgttct gtgtcacgtc ccgcgcccag actgcgtccc cgggcatcac cttgcgcagt
     6361 tgagcgctga acgtgccata ggctcccaat gtctcaatga acactgcctg tgcattgcgt
     6421 ttaaggtcgg cgaattcttc ggcatacccc ggcgcaacct tcaatttgcc ttcgatccgg
     6481 ctgaccaagc cttcaagcgt tgcgttggcg tcgccacaaa caaaatactg gctggcatag
     6541 gtgcggccat ttgccgcggg atcggcatcg acctggatga tattgtcggg aagctgtact
     6601 tggaaatcat tggtttcgtg cccgcgcagc cggcatccca ccaccaggca cagatcgacg
     6661 gtcttgtaga agtcagcaat gatcggcatc ccgatgccgg tcaggccgcc caggttgcgg
     6721 ggatgttcct ccggcaccgt cgcccggccg ttgaaactgg tcaccatccc gaaccccatt
     6781 tgcagcagct tcgacgccgc gctgccggct ttcttcgcgc cgttgcccag ccacagcatc
     6841 ggccgcttcg ccgcgatcac ccgcgacgca agttcatcca gttccgtatc cgtcggcacc
     6901 cggggcgccg gaagcggcag cacgaagtta tccagcatcg ccgggcgttc gatcttggcc
     6961 cgctgcagat cgatcggcac ctcgacactg accggcccca tcggcgcgct gagggcttcc
     7021 atggccgcct tggtcaacag acccagtgca ttctgcgccg accggatgcg ataggcgctc
     7081 ttgcagaccg aagccatcat gccgagctga tcgaccgggt cgtggactga tcccatgcct
     7141 cggtcggcgt atcgggtcga tgtctggccg gtgatgtgca gcacgggcga tccggcgaaa
     7201 cgcgcctcga tcaggccgcc gatggtgttc gccgcacctg gcccggtcga ggaaatgata
     7261 acccccaggc cctcggccac cctggcgtat ccgtcggcca tgtgcgcccc gccgagttcg
     7321 ccgcgtgcca tcatgaagcg gatggtgttg cgccgcccga tcgcgtcgag catgggaatg
     7381 ttatgcaccg atgcgatgcc gaacacggtg gtgacgccgc acgcggacag gaattcggca
     7441 ataagatcgc cgacggtgta gtcgctggcc atggttcaga ctccgctcaa ggttaggtga
     7501 ggctgaaaca gagccttcgt cgcgtcaacg ccgcggcgga accggccgct gcagtacggg
     7561 ctgcgcgacg acaggacggg cggcggctcg cggcgacaac acgatgcaga tggcggcagc
     7621 gagcatgaac aggctggcga ccagcatcgg aacctcgtag ctgccggtcg catcgcgcag
     7681 caggccgacc atctgtggcg ccacatagcc ggaaatgttg ccgatggaat tgatcaccgc
     7741 gatcccgccg gccgccgcgg ctggcccgag ccacgcggtg ggaagattcc agaagcaagg
     7801 caaggaggac gaaataccaa accccgcgag gcagaagcac gccatggcga cgactggatt
     7861 gatcgacaaa gtcaggccgg cgcccaacag cccgacagcg gccagcaggc agggcacggc
     7921 gacatgccag atcctctcgc cggttcggtc ggaattgcgc gagaacagga tcatcccgag
     7981 gcagccaaac agattgggca gcgaagacag gtaaccgacc gtctggtcac tgtaacccat
     8041 cgtcttgatc atcaacggaa ggaagaagtt cttcgaatac acgccgaagc cgatcagcag
     8101 ataccccagg atcagcatca gcatcattgg gctgacgatc gattgcatca gactgagctt
     8161 ctgagtgctc acctgatcgc tctcggcatc cagccgctcc cgcaaccagc tttgttcgct
     8221 acgggtgagc cacgcggcgt cttccggccg atcccgcatc aggattgggc aaagcccggc
     8281 cagcacgcag gcagggatac cttcgatcag gaacagccat tgccagcccg acaggccgtg
     8341 ccagccgtgc aacagcaaaa ggttgccgga aacaggaccg cctatcacgc tggcacttgc
     8401 cccgaacgca tagaatatcc ccatcgcccc accgcgatgc cgcgatggaa accagcgcgt
     8461 catgaaccat gcgacgcctg gaaagaaacc cgattcggca atccccagca ggaaccgcat
     8521 ggtgacgaaa ctggtggcgt cggtaacgaa ggcggttccg gcggagcagg caccccagac
     8581 caccatcgtg acgcccagcc agcgccgtgc cccgatccgg gtcaggatgg cgctggcggg
     8641 aagctggaac aacacatagc tccagaagaa cgcgcccgcc gccaagccgt aggtggttgc
     8701 cgacagcccc agttccttgt tcatcgtgag cgcggcgatt gacacattga agcgatcgag
     8761 cgcgttgatc aggtaggcga agctgaggaa cggaaccagc cgccatgcga ctttgcgcat
     8821 gaccgagttt tcgaagtccg ctgccatggt cgattctctc tgatctggtt cagcccgccc
     8881 gattctctct ggtctggttc agcccgccgg tcgccggcag ggcggcggcc gaagcgggac
     8941 taaccgttca ttcacgcccg agcggatctc gttcggagcg tgacgcacga ggtggctaca
     9001 ccacgccgaa gttgatcagg ttgcgcactg gcgcccccga ttttaactcg gcgaaacctt
     9061 cgttgatctg atccagtttg atttcgccgg acaacaaggt gtccagtttc aaccgcccct
     9121 gccgccacag ttcgagcaac ctgggcatgt ccacccggaa ccggttgccg cccatgctgg
     9181 tgccctggat cttccggtcg cgcaggaaat ccgcgccgtg caactcgatc ttcgtgccga
     9241 acgggatcat gccgatgatc gttgcgacgc cgcccgggcg cagcatggcg aagcactgtt
     9301 cggcggtgat cttcagtccg atcgcctcaa acgaataagg cacgccctgg ccgtccgtca
     9361 ggtccttcac cgcagccacc ggatcgacat tcgaggcatt gatcgtgtcg gtcgccccca
     9421 tctcccgggc tttttccagt ttggatgcat tggtgtcgat ggcgatgatc cgcgcggcac
     9481 ccgcgagggc cgcgccgttg accgccgaca gcccgatgcc gccgcagccg acgacagcga
     9541 cggtgcttcc cggctcgacc ttggctgcgt tgaacacggc gccgaccccg gtcattaccc
     9601 cgcacccgat cagtgccgcc cggtcgagtg ggatgtcctt gttgatcttc accacggcgt
     9661 gctcgtggac cagcatctgt tcggcgaacg ccgacaggtt gaacgcctgg ttcagcaccg
     9721 cgtttttcca cttcagccgg cgtgccgcac ccggcagcag cttcacctcg gtgttctcgc
     9781 acaggttggt gtgaccggtg gtgcactgcg cgcactcgcc gcaaaacacc gacaggcagg
     9841 ttatgacgtg gtccccgggc ttcacgtagg tcacgtcctc ccccacagct tcgaccaccg
     9901 cggcgccctc atggcccagc accgccgggg ccgggaaagg atagagccct tccatgaaat
     9961 gcagatcgga atggcagagg ccggcatagg cggtgcgcaa caacacttcc cggcgcttcg
    10021 gcttctcgat ttgcacgtcc tcgatcgtca gaggctggtg cgctgcgtga aagacagcgg
    10081 ctttcatggt cggttccctc cagtttgatg cgggaggaag actaaccgcc ttggtcgcta
    10141 tcgccaatgc ttctggtaat atggttccgc gtccggccct tccagcatga ttgcatgatc
    10201 ggtggcgaaa tagtccgcgt aatgcagcac gtacgcctcg tgattgcgcc cgtgatacgg
    10261 cgaattcccg gcatgcaatg ccaccgtgct gacgaccgta tggtcgaagc caagctcctt
    10321 cagcaacatc gcgccggcca cgccatgctg cagttcgtgg tatagctttg aataaaccac
    10381 cttgccggcg ccctcccgca gatacataag cggtttgtca acgtcgtgca ggatcaggat
    10441 cggcaccagg acattgaggt cgagcggctt gtcccagtcc gctgccgcgc gcttcgccag
    10501 atccaggccg ttgcgggtca cgtcg
//
LOCUS       DASWQN010000583         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1507539, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000583 DASWQN010000000
VERSION     DASWQN010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507539"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VGC82_09770"
     CDS             <1..520
                     /locus_tag="VGC82_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0423596.1"
                     /translation="FSACYKWMLGITGVAVAYWNRTRQPNWAPSSAGWHSLAEGSRGY
                     DTRPALKSDAMRFTRGNPAHAPIYVLNNALSYLSDFDMTAVQRHVQMLTVALLEELAA
                     SQVAVMTPGDPARHGASVCIASPRASAVVEALYARDVYAWNGQGRVRISFHGYNNISD
                     VERVVAALRVCL"
     gene            868..1248
                     /locus_tag="VGC82_09775"
     CDS             868..1248
                     /locus_tag="VGC82_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423597.1"
                     /translation="MRVGIWLGIGLTVFPGFAFADASVAGYWTADLGDRVTIEMNLGE
                     DGQWNSTTAKANETIAEMAGKYQQQVQSPTSGRLIFVPSQAHVTSQHGAPKIEHDVYR
                     LSSDGRVLSLTASGDTMEFHKLTP"
     gene            1262..1564
                     /locus_tag="VGC82_09780"
     CDS             1262..1564
                     /locus_tag="VGC82_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423598.1"
                     /translation="MRAVLLIIAVIATLGMGGARPAYAIGCISGGLLGGAAGHLANHG
                     VLGAIGGCVAGHQLHKRQQQQDDLRQQQMQMNQQQYNNGGSNNGGYNNGGYNNRYQ"
     gene            complement(1681..2601)
                     /locus_tag="VGC82_09785"
     CDS             complement(1681..2601)
                     /locus_tag="VGC82_09785"
                     /inference="COORDINATES: protein motif:HMM:NF006094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEY0423599.1"
                     /translation="MIDRKRVAEAAERISKHVRHTPVMQGDLGTDSLVTLKLELLQHA
                     GSFKPRGAFNRLLTAALPPAGVIAASGGNHGAAVAYAARALGVTAEIFVPAGTPTAKV
                     ARIASYGAHVVRGGEAYAEALLASRSRQAESGALEVHAYDDDAVLAGQGTVGRELEQD
                     APDLTHILIATGGGGLIGGVAAWYAGSAQIISVEPEGCPALHDALRAGQPVAAPVGGV
                     AADSLGARQVGRLMFPIARHFVADAVLVPDDAIVAAQLLLWDRFRLIAEPGGATAAAA
                     LLSGRFRPPAGARVGVVVCGANTDPTKLGG"
     gene            2681..3115
                     /locus_tag="VGC82_09790"
     CDS             2681..3115
                     /locus_tag="VGC82_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0423600.1"
                     /translation="MHSQRRMHGSCVSRDGDAILVVGPPGSGKSDLVLRLLSRGFELV
                     ADDQVDITDDIASCPAELAGLLEARGVGIVRLPYRTCARLVLVVELDKRIERMPVPMR
                     HSDLQLPLVRVDPTAASAPEKVALALDCVTGRVSQVAGAFQP"
     gene            3112..>3296
                     /locus_tag="VGC82_09795"
     CDS             3112..>3296
                     /locus_tag="VGC82_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010503493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY0423601.1"
                     /translation="MTKMPVVIVTGLSGGGKASILRALEDVGYEAVDNPPLTMLEEMI
                     VRGDRRLAVGIDARTRG"
BASE COUNT          607 a         1042 c         1035 g          612 t
ORIGIN      
        1 cttctccgcc tgctataaat ggatgctcgg tattaccggc gtcgccgtcg cctactggaa
       61 ccgaaccagg cagccgaact gggcgccttc atccgctggc tggcactccc tggccgaggg
      121 ttcgcgcggc tacgatacac gtccggcatt gaaatccgac gccatgcgct ttacccgcgg
      181 caacccggcg catgccccga tctacgtgtt gaacaacgcc ctgtcgtatc tgtcggattt
      241 tgatatgacg gccgtgcaac ggcatgtgca gatgctgacg gtggcgctgc tggaggaact
      301 tgccgcttct caggttgcag tcatgacgcc cggcgatccg gcgcggcatg gggcgagtgt
      361 ttgtatcgcc tcgccgcgag cttcggcggt tgttgaggcg ctgtatgcac gggatgtgta
      421 tgcatggaac ggtcagggcc gggtgcggat cagcttccac ggctacaaca acatatccga
      481 tgtagagcgg gtcgtcgcgg cattgcgggt ttgcttatga gttccgcgca gaacagcgac
      541 cggcggcgcg cttctgggca atgacgaccc ggtcccctgt tcgatcctgg atccccgaat
      601 cgaagcagca ctgtcatgtg aaacggcttt gaccgcaccg gtggccttgc ggatggcgtg
      661 gtatcgatgg aggtatttgg tgttttagtg caacaagtct caccacttct tgggtactga
      721 cagcgtaagc cgccctcggt tgcgccgcga gtcgtgcgcc gccacgctct ggtgctgcca
      781 accgtccggg acggcatccg gtggtccctg gacagcatga ccacccattt attatcgtcc
      841 cagcgcactt tatcgaggaa taccgacatg cgcgtaggca tatggcttgg tatcggcctg
      901 acggtctttc ccggtttcgc cttcgctgat gcgagcgtgg cgggatactg gaccgcggat
      961 ctgggcgacc gggtgacgat cgaaatgaac ctaggcgaag atggccagtg gaacagcacc
     1021 actgccaaag ccaacgagac catcgccgag atggcgggaa aatatcagca gcaggtgcag
     1081 tcgccaacga gcggccggtt aatcttcgtc ccgagccagg cgcacgtcac gtcgcaacat
     1141 ggtgccccca aaatcgaaca cgacgtttat cgcttatcga gcgacggacg ggtgctcagt
     1201 ttgacagcct ccggcgatac gatggaattc cacaaactga ccccctgaga cggagacaac
     1261 aatgcgtgcc gttttgctca tcatcgctgt gattgctacg ctcgggatgg gtggcgccag
     1321 accggcgtac gctatcggct gtatcagcgg tggcctcctt ggcggcgcgg ccgggcatct
     1381 ggccaatcat ggcgttttag gcgccatcgg cggttgtgtt gctggccatc aactgcacaa
     1441 acgtcaacag cagcaggacg acctacgtca acagcagatg caaatgaatc agcagcagta
     1501 taacaatggc ggatctaaca acggtggcta caacaacggc ggctacaaca atcgatatca
     1561 gtaagtgaac ggcacgcaaa acgcccgcgc gacagcaccg ccataggttg gaagaaacac
     1621 ggccgcgttc cgggcgacgc acatctttaa cgctgtggct ggtaacgctg tttggctggg
     1681 tcatcccccg agcttcgtgg gatcggtgtt cgcgccgcac acgaccaccc cgacccgagc
     1741 gccggcaggc gggcgaaacc ggcctgaaag cagcgcggcg gcggcagtcg cgccgcctgg
     1801 ttcggcgatc agccggaagc ggtcccacag cagaagctgg gccgccacga tcgcatcgtc
     1861 cggcaccagc acggcgtcgg ccacgaagtg cctcgcgatt gggaacatca gccggcccac
     1921 ctgcctggcc cccaggctgt cggcggccac cccgcccaca ggcgcggcga ccggttgccc
     1981 ggcccgcaaa gcgtcgtgca acgcagggca accttccggc tccacgctga tgatttgggc
     2041 gctgcccgca taccaggccg cgactccacc gatcagtcca ccgcccccgg tggcgatcag
     2101 gatgtgagtg aggtccggcg cgtcctgctc cagttcacgc cccacagtac cttggccagc
     2161 caagaccgcg tcgtcgtcgt aagcgtgcac ctccagagca ccgctctccg cttggcgcga
     2221 ccgggaagcc agaagcgcct cggcataggc ttcaccaccc cggacgacat gcgccccgta
     2281 gctcgcgatt cgcgcgacct tcgctgtggg cgtgccggcc gggacgaata tctcggccgt
     2341 cacgcccaag gcccgcgccg cataggcaac cgccgcgcca tgattgccac ccgacgcagc
     2401 gatgaccccg gccggtggca gcgctgccgt cagcagccgg ttgaacgccc cccggggctt
     2461 gaagctgccg gcatgctgca gcagttccag cttcaacgtg acgagcgagt cggtgccgag
     2521 gtcgccctgc atgacaggcg tatggcggac atgcttcgat atgcgttcgg cggcctccgc
     2581 cacccgtttt cgatcaatca tcaccgcctg acgctggcat gggcacacat ccccgacaag
     2641 ccgctttctt tttgtgcatc gcacgcccac aatcgatctc atgcacagcc agcgtcgtat
     2701 gcacggaagc tgcgtgtcca gggacggaga tgcgatcctg gtggtgggtc caccagggtc
     2761 cggtaaatcc gaccttgtcc tgcggcttct ctcgcgcggc ttcgaactgg tggcggacga
     2821 tcaggtggac attacggacg acatagcatc ctgtccggcc gaattggctg gcttgctcga
     2881 ggctcgcggc gtggggatcg tgcggctgcc ataccggacc tgcgcccgcc ttgtgctggt
     2941 tgtcgagctt gacaagcgca tcgagcgtat gccggtgccg atgcgccatt ccgatttgca
     3001 gctaccactc gttcgtgtcg atcccactgc ggcgtcggca ccggaaaagg tggcccttgc
     3061 gctggattgc gtgacgggcc gggtcagtca ggttgccggg gcatttcagc catgaccaaa
     3121 atgcctgtcg tgattgtcac cggactttcc ggtggaggaa aagcgtcgat cctgcgggcg
     3181 ctggaggacg tcggatatga agcggtggac aacccaccgc tgacgatgct ggaggaaatg
     3241 atcgttcgcg gcgaccgtag gctggcggtc ggcatcgatg cgcggacacg cggttt
//
LOCUS       DASWQN010000584         2914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_320897, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000584 DASWQN010000000
VERSION     DASWQN010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320897"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1928)
                     /gene="selB"
                     /locus_tag="VGC82_09800"
     CDS             complement(45..1928)
                     /gene="selB"
                     /locus_tag="VGC82_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEY0423602.1"
                     /translation="MIIGTAGHVDHGKTALIKALTGVDTDRLAEEKRRGITIDLGYAY
                     TDDFGFVDVPGHERFVHTMLAGASGIDTALLVVALSEGIRPQTREHLQILSLLGIDRG
                     VVALTKADLAGDRIAEVTGQLQALLAGTPLETAAMIPVSAVTGQGIGDLRALLLASAG
                     PARDEGGYPRLAVDRVFTLPGAGLIVTGTLVAGRITVEDRLVLSPSGLELRVRGLHAQ
                     NKPADRAVAGQRVALNITGPRLSKDDVTRGDWVVHPDIHAPTTGIDARIALLPTVSRA
                     MRQDTNVHLHLGAAHVMARVSLLNQERLEPGSVALARLTLQQPIGALAGDKIVLRDTG
                     ATCTIGGGLILDPFPPRRGRRTAMRLAQLATMEAPETLDALRGLLAVSPGWTDGGLFM
                     RGRNVPEAERAALIAATPAVAAGGLLLSTGAFDFIRKTVLAILADHHSASPELPGLQA
                     ERLRQALTERPPVMGFAGILDLLRQAGAITQDGVWFRLPGHRISLSPQDEKLWRAARP
                     SVAADRFRPPRTRDLAAALKVPEAAMRTTLKRLARMGQVVEIAPDHFFLRETITEMAA
                     LAVAAVDADGLLTAAAFRDRLGNGRKVAIQILEFFDKTGITVRSGDVRRVRMDRLGLF
                     GQP"
     gene            2086..2535
                     /locus_tag="VGC82_09805"
     CDS             2086..2535
                     /locus_tag="VGC82_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY0423603.1"
                     /translation="MMQTTIYRLLPASLRRSATPIRLDVAAQFIRFGVVGLSGLIIDT
                     ATVYGLRHTLGLYGAGLVAYLVAATGNWILNRIWTFRGQGTGSVHRQWAMFMVANLAG
                     FVLNRGAYVLMVTFIAAAAEQPVIATASGAVAGLLVNFTLSRRIVFR"
     gene            2685..>2914
                     /locus_tag="VGC82_09810"
     CDS             2685..>2914
                     /locus_tag="VGC82_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423604.1"
                     /translation="MSAFPPRALETLAYSSGGLSLAPMSFRHAGAMDPRDAVLKRTAD
                     IILALCGLILMGIPMLLAALAIRMDSQGPALFR"
BASE COUNT          500 a         1041 c          848 g          525 t
ORIGIN      
        1 gtttctgtcg gccggttgcc tttctgtcct tccgcgggct tctttcaggg ctggccgaac
       61 agccccaacc gatccatccg tacccgccgc acgtcgcccg atcgaaccgt gataccggtc
      121 ttgtcgaaga actccaggat ctggatcgcc accttccgtc cattacccag gcggtcgcga
      181 aacgccgccg ccgtcagcag cccgtcagcg tcaaccgccg ccaccgcgag agccgccatc
      241 tccgtaatcg tttcgcgcaa gaaaaaatga tcgggcgcaa tctctaccac ctgccccatt
      301 cgcgccaacc gcttcagcgt tgtccgcatc gccgcctccg gtaccttcag agccgcggcg
      361 agatcgcgcg tccgaggcgg ccgaaaccgg tccgcggcaa ccgacggccg tgctgcccgc
      421 cacagtttct catcttgcgg cgacagggaa atccgatgac cgggcaggcg gaaccagact
      481 ccatcctggg tgatcgcccc cgcctgccgc aacaggtcca gtatccccgc gaaccccatc
      541 accggcggcc gctccgtcag cgcctgccgt agccgctcgg cctgcaagcc cggcaattcg
      601 ggcgatgccg aatggtgatc cgccaggatc gcgagaactg tcttgcgaat gaaatcgaac
      661 gcccccgtag acagcagcaa cccgcccgcc gccacggccg gtgtcgcggc aatcaaagcc
      721 gcccgctccg cctcgggtac gttacggccg cgcatgaaga gaccgccgtc cgtccagcct
      781 ggcgagaccg ccagcaaccc ccgcagggca tccagggttt ccggcgcctc catcgtagcc
      841 agttgcgcca gccgcatcgc agtccgccgc cctcgccggg gcgggaaagg atccaatatc
      901 aggccgccgc cgatcgtgca ggtcgcgccg gtatcccgca gcacgatttt gtcgcccgcc
      961 agtgccccga tcggctgctg caacgtcagt ctcgcgaggg ctaccgaccc cggctccaac
     1021 cgctcctggt taagcagcga cacacgagcc atcacatgcg ccgcgcccag atggagatgc
     1081 acatttgtat cttgccgcat tgcccgcgac acggtcggca gcaatgcgat ccgcgcatcg
     1141 attccggttg ttggcgcatg aatatccggg tgcaccaccc agtcgccgcg ggtcacatca
     1201 tccttcgaga gccgaggccc ggtgatgttc agcgccactc gctgcccggc caccgctcgg
     1261 tcggcgggct tgttctgggc gtgcagcccg cgcacccgca actccagccc ggacggcgac
     1321 aacacgagcc ggtcttccac cgtgatccgc cccgcgacca acgtgccggt cacgatcagt
     1381 cccgcacccg gcagtgtgaa cactcggtca accgccagcc gcggatatcc gccctcgtcg
     1441 cgcgccggcc ccgccgatgc cagcaacagc gcgcgcaggt cgccgatccc ctgtccggta
     1501 acggccgaga ccgggatcat cgccgccgtt tcaagcggcg ttccggccag caaggcctgc
     1561 aactgacccg tcacctcggc gatgcggtcg cccgcgaggt cggccttcgt cagggcaacg
     1621 acgccccggt cgatgcccaa cagcgacaat atctgcaaat gctcgcgcgt ctgtggccgg
     1681 atcccctcgg acagcgccac caccagcagt gcggtgtcga tgccgctggc cccggcgagc
     1741 atggtatgca cgaaccgttc atgcccgggt acatcgacga agccaaagtc atcggtgtag
     1801 gcatagccca ggtcgatggt gatgccgcgc cgcttttcct cggccaggcg atcggtatcg
     1861 acgcccgtca gcgccttgat aagcgcggtc ttgccgtgat cgacgtgtcc ggcagtgccg
     1921 atgatcatca gaaggtccgg ttcgcttgaa cggcattagg gagaaaccga cccttgattg
     1981 ctgtcaaccc cggatgcttt cggcccatgc tcgaataggg gtgttggatg cttgcgacgt
     2041 tccgcctgat cgcgttacgc aggaaaccga acaggcggaa agcgcatgat gcagaccacg
     2101 atctaccgtc ttctgccagc ttcgctgcga cgatccgcga cgccgattcg gctcgacgtt
     2161 gccgcgcaat tcatccggtt tggcgtggtc ggcctgtccg gcctgatcat cgataccgcg
     2221 acggtttacg gcttgcgcca cactctgggg ctgtacggcg ccggcttggt cgcatatctg
     2281 gtagcggcca ctggaaactg gatactcaac cgcatatgga cgttccgcgg ccagggaacg
     2341 ggatcggtgc accggcagtg ggcgatgttc atggtcgcca atttggccgg cttcgtattg
     2401 aaccgcggcg cctacgtgct gatggtgacg tttatcgccg ccgccgccga gcagccggtc
     2461 atagccaccg cctccggtgc tgtcgccgga ttgctggtga atttcactct ctcccgccgc
     2521 atcgtgtttc gctgacccgc cacaggttcg ttgtgtgttc ctaacattgt ggctggcata
     2581 gaagaaaaaa tcgcgccgtt gtacatccgt gtgcggcgtt aacggctctc taatcaattc
     2641 ccgccgaaac tcctgctcgt cgcaaccagc agatgcagca gatcatgtcc gccttccctc
     2701 cccgagcact cgaaaccctg gcctacagca gcggcggatt gtcgctggca ccgatgtcgt
     2761 ttcgccatgc cggcgcgatg gacccgcgtg acgccgttct caagcgaacc gcggacatca
     2821 ttctcgcact ttgcggcctg atactgatgg gcatccccat gcttcttgct gccctcgcga
     2881 tccgcatgga cagccagggc cccgctctgt tccg
//
LOCUS       DASWQN010000585         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_715649, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000585 DASWQN010000000
VERSION     DASWQN010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715649"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..932
                     /locus_tag="VGC82_09815"
     CDS             51..932
                     /locus_tag="VGC82_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423605.1"
                     /translation="MARDYYESEWPDYVSVSERRAKAERAAAKLSKKGSSLSPVIVEG
                     RKLASTFWGKAWCDNLESYRDYDNRLERGRSYVRNGLVIDLQIEPRTITAMVSGSSIY
                     KIVISITEMPKAQWKSICADCAGGIDSLVELLQGRFSKPVMERICRQGTGLFPKPTEI
                     RFSCSCPDHASMCKHVAATLYGVGARLDARPELLFRLRAVNENDLVADIGSALPMGKQ
                     APAAGKVLETDDMAALFGLDMGDVPQPAKAVRPVTATKTKTPAMAGIATPKVTTHKPV
                     VAKKKSAVSKRSAATGR"
     gene            1020..1811
                     /locus_tag="VGC82_09820"
     CDS             1020..1811
                     /locus_tag="VGC82_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY0423606.1"
                     /translation="MHTEWRKLRADQLREQARRNAIVILPVASLEQHGPHLPVEVDSI
                     LGETVAARTAEKVLAAGQPVLVLPVLWTGLSEHHMSFGGTVTLDNAAFAALIEGVIRS
                     VLRHGFRRIVLLNAHGGNENALRTITDDLTPKLGVPIVQFTYWYAAAVAIAKILETQG
                     GLSHACEAETSMMMAVRPELVALDRIPLAKSNTTPALDDVVGGGVHMWRSVGSRSASG
                     VLGNPEAATPEKGVALFESISSVLAGKLCNPDLWDLPRTSETVQT"
     gene            1866..2333
                     /locus_tag="VGC82_09825"
     CDS             1866..2333
                     /locus_tag="VGC82_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0423607.1"
                     /translation="MGIDLVVNGRNVSIATDPTTPLLDVLRNHLDLKGSRYGCGLEQC
                     GTCMVLVDGEPVYSCAREVGSISGRSVTTIEGLGSPEAPHPLQRAFLDEQAGQCGYCL
                     SGIIISAKALLDRKPKPTRADIVAALDKHLCRCGTHTRIIAAVQKAASMGALA"
     gene            2330..>2771
                     /locus_tag="VGC82_09830"
     CDS             2330..>2771
                     /locus_tag="VGC82_09830"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0423608.1"
                     /translation="MSETLPLSIQNNRRLEKWLRFQSDRAVRLAVGKVEIGQGVLIAL
                     SQIAAEELDVAIDRIDILSGDTDDAPDEGSTSSSQSIEVSGRSVRLVSAELRTRVLGR
                     LAQRLNCAMEDLAIEDGVFLQDGNATGYDYWSFSGPEDFAAEIEG"
BASE COUNT          534 a          824 c          926 g          487 t
ORIGIN      
        1 atgcggctgg tcgcattgga tctgaatgcg gcgatgaggg agtaacagcc atggcacggg
       61 attattatga aagtgaatgg ccggattatg tgtcggtatc agaacggcgt gccaaggctg
      121 aacgtgcggc ggcaaagctg agcaagaagg ggtcgtccct gtcgccggtc atcgttgaag
      181 gccgcaagct tgcctcgaca ttctggggca aggcgtggtg cgacaacctg gagagttacc
      241 gcgactacga caaccgcctg gaacgcgggc gtagctacgt gcgcaacggg ctggtgatcg
      301 acctgcagat cgagccgcgc acgataacgg cgatggtcag cggatcatcg atctacaaga
      361 tcgtcatcag tattaccgag atgccgaagg cgcagtggaa atcgatctgc gccgattgcg
      421 cgggcggcat cgattcgctg gttgaactgc tgcagggccg gttttccaaa ccggtgatgg
      481 agcggatatg ccgtcagggg accgggctgt tcccgaagcc cacggagatc aggttttcct
      541 gcagttgccc cgaccacgcc tcgatgtgca agcacgtcgc cgccacgctt tacggcgtcg
      601 gcgctcggct ggatgccagg ccggagctgc ttttccgcct gcgtgcggtg aacgagaacg
      661 acctggtggc ggatatcggt tcggcgttgc cgatgggaaa gcaagctcct gcggcgggaa
      721 aggtgctgga aaccgacgat atggcggcgc tgttcggtct ggacatgggc gatgtgccgc
      781 agcccgccaa ggcggttcgt cccgtcaccg cgacgaagac aaaaacccct gccatggcgg
      841 gtatcgcgac gccaaaggtc acaacccaca agccggtcgt agcgaagaaa aaaagtgccg
      901 tgagcaagag atctgccgcc acggggcggt gaacgcgtta ctcgcgcggc atcgccgcct
      961 tcccactggc atatccgcgt ggcctcctgt tccaatcgtt gaagtgggga ccgatcatca
     1021 tgcacaccga atggcgcaag ctgcgcgctg accaactgcg ggaacaggcg cggcgcaatg
     1081 cgattgtcat cctgccggtt gcctcgctgg agcagcatgg cccgcacctc ccggtcgagg
     1141 tcgactcgat actgggagag acagtggccg cgcgaaccgc ggagaaggtg ctggcagccg
     1201 gtcagccggt gttggtcctc ccggtgctgt ggaccggcct gtccgaacat cacatgagtt
     1261 tcggcggcac tgttacgctg gacaacgccg ccttcgctgc gctgatcgag ggcgtgatcc
     1321 gttccgttct gcggcatggc ttccgccgca tcgtgctgct gaacgcccat ggcggcaacg
     1381 agaatgccct gcggaccatc accgacgacc tgacgccaaa gctcggcgtg ccgatcgtcc
     1441 agttcaccta ctggtatgcc gccgcggtgg ccattgcgaa aatcctggaa acccaggggg
     1501 gcctgagcca cgcctgcgag gccgagacct cgatgatgat ggcggtacgc cccgaactgg
     1561 tggcactcga caggataccg ctggcgaaat ccaacaccac gcctgccctc gacgacgtcg
     1621 tgggcggtgg ggtccacatg tggcgcagcg tcggcagccg atccgcctcc ggcgttttgg
     1681 gcaaccccga agcggcgacg ccggagaaag gcgttgcgct gttcgagtcg atatcgtcag
     1741 tgctggccgg aaagctgtgc aatcctgacc tgtgggattt gcccaggacc tcggagacgg
     1801 tccagactta gcgcgtgacc gggtgatcga gccacgcgga tcaataaacg agacagggga
     1861 cacggatggg catcgatctg gtcgtaaacg gccgcaacgt cagcatcgcc accgatccga
     1921 ccacgccgct gctggacgtg ctccgcaatc acctggacct caaaggctcc cgctacggat
     1981 gcgggctgga gcagtgcggc acctgcatgg ttctggtcga tggtgagcca gtctattcct
     2041 gcgcgcggga ggtcggttcg atctccgggc gctccgtgac gacgatcgag gggctgggat
     2101 cgccggaagc gccgcatccc ttgcagcgcg cattcctgga tgaacaggcc ggccagtgcg
     2161 gctactgcct gtccgggatc atcatcagcg ccaaggccct gctggaccga aaaccgaagc
     2221 cgacgcgagc cgatatcgtc gcggcgttgg ataaacatct ttgccgctgt ggcacgcata
     2281 cgcggatcat cgccgcggtc caaaaagccg ccagcatggg agcgttggca tgagcgagac
     2341 gttgccgctg agcatccaga acaatcgccg gctggagaaa tggctgcgct tccagtccga
     2401 ccgggccgtc cgcttggccg tgggcaaggt cgaaatcggc caaggcgtgt tgatcgccct
     2461 gtcgcagatc gcggccgagg aactggatgt ggcgatcgac cgcatcgaca tcctgtccgg
     2521 cgacaccgat gacgcgccgg atgaaggatc gacgagttcc agccagtcga tcgaagtgtc
     2581 cggccgttcg gttcgcctgg tcagcgcgga actacgcact cgggtgttgg gccgcctggc
     2641 gcaacggctg aactgcgcaa tggaggattt ggctatcgag gacggtgtgt ttcttcagga
     2701 cggcaacgcc acgggctacg attactggag cttctccggt ccagaggatt tcgcggccga
     2761 gatagagggt g
//
LOCUS       DASWQN010000586         4529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_57324, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000586 DASWQN010000000
VERSION     DASWQN010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57324"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1179
                     /locus_tag="VGC82_09835"
     CDS             148..1179
                     /locus_tag="VGC82_09835"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0423609.1"
                     /translation="MKIGLGTWRFFLASLVAISHLWAGMIDGPAAYAVWGFFVLSGYL
                     MTLVLTTRYGNTIGGLRQFAFNRLLRIYPSFLAAGALGIATILGLGCLGIDPSGLNPQ
                     FMLPQGIGEWLSNLGMVPFLPQQGLPVPVAAALFVEVWAYALMPLFASGKSAAVLGLA
                     VAFFANLQLGFGMPTFIARYCGFATGLMPFAVGAVVCHYRASLRRFAHPTLSVAVWCL
                     HGLYWLADVYWPWTYGLFVSVPLSAWVVLSLAGSNSGAADKFAGELSYPVYLLHTTVA
                     AWFLPQFGFRRSFMFFAAAFAATLIASWGMLRLIDTPVQRLKLRSGTFRRAASRGLPS
                     PEPAARALS"
     gene            complement(1222..2379)
                     /gene="rlmN"
                     /locus_tag="VGC82_09840"
     CDS             complement(1222..2379)
                     /gene="rlmN"
                     /locus_tag="VGC82_09840"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY0423610.1"
                     /translation="MSDLSEADRARVLAKSALFDPPAVALADGRRELVGMDRTELAAE
                     MAAIGEPAFRVKQLWHWIYHQGVTDFSRMSSISRSLQEKLAERFVIGRPEAATVQTSV
                     DETRKFLFRFRDGQEAETVYIPDPVEDRGAVCISSQVGCTLSCRFCHTGTQTLVRNLG
                     APEIVGQFMAARDSYGEWPSPKGETPRLLSTIVLMGMGEPLYNYTNVAKAMKIVMDGE
                     GIGLSRRRITLSTSGVVPMMDRAGAELGVNLAVSLHAVTDQLRDEIVPLNRKYPIAEV
                     LAACRRYPGSSNARRITFEYIMLKGINDSEAEARELVRLIAGMPAKVNLIPFNPWPGS
                     KYEPSGKAALERFAAIVMAAGFAAPIRTPRGQDILAACGQLRTDSRRDRAG"
     gene            complement(2376..2885)
                     /locus_tag="VGC82_09845"
     CDS             complement(2376..2885)
                     /locus_tag="VGC82_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423611.1"
                     /translation="MTLALLLALPAAAESKNAAPPAHRAAAASAPKQIGKFDDWTAAT
                     HSEAGETVCYAFTRAQNSVPALPGRGPVVLTVTERSSGRDSVAIDAGFSYPPNATVTL
                     QVDQAGLEFYTSGRNAFAHDGRAAVAAFGKAGRAISRSPGTKEVTDTFSLKGFGSAHT
                     AIVKACPAK"
     gene            complement(2957..3535)
                     /locus_tag="VGC82_09850"
     CDS             complement(2957..3535)
                     /locus_tag="VGC82_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0423612.1"
                     /translation="MVVARTRGYFGIGAEGVSKSANVGALLRTGHAFGASFCFTIGSG
                     WDSRAARTADTADTPSHVPMWRYPSLTELSLPHECVLVGIELLDDAVDLPSFRHPLNA
                     AYVLGPERAGLSAALLERCDHVVRIPTRFALNLAVAGALVLYDRMLQHGRFAERPVGS
                     RGQIEALAPTGHGAPRFRKTVPDWLNVNAKKP"
     gene            3705..>4529
                     /locus_tag="VGC82_09855"
     CDS             3705..>4529
                     /locus_tag="VGC82_09855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003628814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0423613.1"
                     /translation="MTDPTDQRLAGQYEAYPYPARNPRDEARRLIVGSPSHIREIDHW
                     VFGAARHASRPLNALVAGGGTGDATIMLAQQMTTLERPGRVTWLDRSAAALAIAKGRA
                     QARGLTNIVWEQRSLLDLPGSGLGPFDYIDCCGVLHHLLDPAMGLRALLSVLAPGGGM
                     GLMVYAPHGRTGVYMMQDALRRLAPPEETPQQRLDIARRVMKHLPETQWLRHNRNFDD
                     HVNGGDAGLYDLLLNPRDRAYTVPELAVLLRDAGLRVTCWVEPIRYDPAPLLPDPRL"
BASE COUNT          776 a         1551 c         1356 g          846 t
ORIGIN      
        1 cggatgggtc gggggtgcaa gcgaaccgtc ggccatgcgg gttgaagtac cacgccggcg
       61 cagtaacgtt tggtcttttg tttaagcgtg attgagcggc cgttccggtg tagatcatac
      121 cctgtatgca accagtgcgg aaatgtcatg aaaatcggcc tcgggacctg gcggttcttt
      181 ctcgcgtccc tggtggcgat ctcgcatctg tgggctggca tgatcgatgg cccggcggcc
      241 tatgcggtat gggggttttt cgtcctcagc ggctacctga tgaccctggt gctgacaacg
      301 aggtatggaa acacgatcgg cgggctgagg cagttcgcgt tcaaccggct gctgcgcatt
      361 tacccgtcgt tcctcgcggc gggcgccctg ggcatcgcga ccatccttgg gctcggttgc
      421 ctcgggatcg acccgtccgg cctgaacccg cagtttatgc tgccgcaagg gatcggcgaa
      481 tggctgagca acctgggaat ggttccattc ctgccgcagc aaggacttcc ggttcccgtg
      541 gcggcggcgc ttttcgtgga ggtctgggcc tatgcgttga tgccactgtt cgcctcgggc
      601 aagagcgccg ccgttctcgg actggctgtg gcgttcttcg ccaatctgca actcggcttc
      661 gggatgccca cctttatcgc gcgctattgc ggatttgcca ccgggctgat gccgttcgcg
      721 gtcggcgcgg tcgtctgcca ttatcgcgcc agcctgcggc ggttcgccca ccccaccctg
      781 agcgtcgctg tctggtgctt gcacggactc tattggctag ctgacgtgta ctggccgtgg
      841 acgtatgggc tgtttgtatc ggtcccgctg tccgcgtggg tcgtgctgtc gctggctgga
      901 tcgaattctg gcgcagccga taaattcgcc ggcgaactat cctacccggt gtatcttctg
      961 cacacgacgg tggcagcgtg gtttctgccc cagtttgggt ttcggcgaag cttcatgttc
     1021 tttgcagccg cgtttgccgc gaccctcatc gcatcatggg ggatgcttcg gctgatcgac
     1081 acgcccgtcc aacgtctcaa actacggtcc gggacctttc gccgtgccgc ctcccgcggc
     1141 ctgccgtcac cggaaccggc tgcgcgggca ctttcctgag ggccggtgcg tcgcagtggg
     1201 cgccagtccc gagcgcgttc gctaccccgc cctgtctcga cggctgtccg tccgcaactg
     1261 gccgcaagcc gccagaatat cctgtccccg aggcgtccgg atcggcgcgg cgaaccccgc
     1321 cgccatcaca atcgccgcaa accgctccag agccgccttg ccgcttggct catatttcga
     1381 ccccggccat ggattgaacg ggatcaggtt caccttcgcc ggcatccccg cgatcagccg
     1441 caccagttcc cgtgcctcag cctcggaatc gttgatcccc ttcaacatga tatactcgaa
     1501 ggtgatccgc ctggcgttcg aagaccccgg ataccggcgg caggcggcca gcacctcggc
     1561 gatcggatac ttccggttca gcggcacgat ctcatcgcgc aactgatccg tcacggcgtg
     1621 cagcgacacc gcgaggttca cccccagctc cgcccccgcc cggtccatca tcggcacaac
     1681 accggacgtg gacagcgtga tccgccgccg cgacaaccca ataccctcgc cgtccatcac
     1741 gatcttcatc gccttggcga cgttcgtgta attgtacagc ggctccccca tccccatcag
     1801 cacgatggtg gacagcaggc gcggagtttc tcccttgggc gacggccatt cgccgtacga
     1861 atcccgagca gccatgaact gaccgacgat ctctggtgcg cccaggttcc gcaccagcgt
     1921 ctgtgtcccg gtatggcaga accggcacga caaggtgcag ccgacctggg acgaaataca
     1981 aaccgcgccc cgatcctcca ccgggtctgg gatatagaca gtctccgcct cctgcccatc
     2041 gcggaaccgg aacaagaatt tgcgggtttc atcgacgctg gtctgcaccg tggccgcttc
     2101 cggccgtccg atcacgaacc gctccgccag cttctcctgc aacgagcgcg agatcgagga
     2161 catgcgcgaa aagtcggtca cgccctgatg atagatccag tgccacaatt gcttgacgcg
     2221 gaacgcgggt tccccgatcg ccgccatctc ggcggccagt tcggtgcggt ccatcccgac
     2281 cagctcccgc cgcccatcgg ccagcgcgac agccggagga tcgaatagcg ccgacttcgc
     2341 cagaacccgc gcgcgatccg cttcggaaag gtcactcatt tcgccgggca cgccttcacg
     2401 atggctgtat gagccgaccc gaatcccttc aggctgaagg tatccgtcac ctccttagtc
     2461 cccggagatc gagaaatcgc tcggccagcc ttgccaaacg cggcaaccgc cgccctgccg
     2521 tcatgtgcga aagcattacg tccggacgtg tagaactcca accccgcctg atcgacctgc
     2581 agcgttacgg tggcattcgg cggataggag aatcccgcgt cgatcgccac ggaatcgcgg
     2641 cctgaggatc gctcggttac ggtcaggacg actgggcccc gcccaggcaa agccggcacc
     2701 gaattttgcg cccgggtgaa ggcgtagcag accgtctctc cggcttcgga atgcgtcgcg
     2761 gccgtccagt cgtcgaactt tccgatctgt ttcggggcag aagcggcggc agcccggtgg
     2821 gcaggtggcg cggcattttt ggattcagcc gccgcgggca gggccagcag cagcgcaagc
     2881 gtcattgaat atcgaaatcg gttttgcatg cgcgcagcaa taaggctgcc gcccacaccg
     2941 aacaagtaca agaaattcat ggcttcttag cgttaacgtt aagccagtcc gggacggtct
     3001 tgcggaaccg cggtgccccg tgcccggtcg gcgccagcgc ctcgatctga ccccgcgagc
     3061 caaccgggcg ttcggcaaaa cgtccatgct gcaacattcg gtcatacagc accaaggcgc
     3121 ccgccaccgc caggttgagt gcgaaccgcg tcggtatccg caccacatga tcgcaccgct
     3181 ccagcaacgc agccgacagt cctgcccgct ccggtcccag cacataagcg gcgttcaagg
     3241 gatgccggaa cgatggcaga tcgacggcgt catccagcaa ctcgatcccc accaggacgc
     3301 actcatgcgg caaggataac tccgtcaacg acggataacg ccacattggc acatgcgatg
     3361 gcgtgtcagc cgtgtcagcc gtccgcgccg cacgggaatc ccaccctgat ccgatagtga
     3421 agcagaacga cgctccgaat gcgtgcccgg tccgcagcag tgcgccgacg ttggccgact
     3481 tggagacccc ctccgcgcca atgccgaaat aaccccttgt tctggctaca accataccag
     3541 cggtgtaaac caattccggg acggcgacac cgttaggccc ggggttggtc gccgaacctg
     3601 ctcagacatt ccgtggacgc cgtcatgcta cgtttcgttg gatcgcgtga ttcacacgct
     3661 tcggacgttc cgcccaccgt gatcatgctc tatttcctgg cgccatgacc gacccgacag
     3721 atcaacgcct cgccggccag tatgaagcct atccttatcc cgcgcggaac ccgcgcgacg
     3781 aggccaggcg cctgatcgtc ggcagcccca gccacatccg ggagatcgac cactgggtgt
     3841 tcggcgcggc ccgccacgcc agtcgcccgc tcaacgcctt ggtcgccggc ggcgggaccg
     3901 gcgacgcgac catcatgctc gcccagcaga tgaccacgct tgaacggccc gggcgggtga
     3961 catggctcga ccgctccgcc gcggcgctgg cgatcgccaa aggtcgggcg caggcgcgtg
     4021 gactgaccaa catcgtgtgg gaacagcgat ccttgctcga cctgccaggt tcaggcctgg
     4081 ggccattcga ctacatcgac tgctgtggcg tcttgcacca cctgctcgat ccggccatgg
     4141 gcctgcgcgc gctgctgtcg gtcctggcac cggggggcgg gatgggtctg atggtctacg
     4201 cgccgcatgg gcgcaccggc gtttacatga tgcaggatgc gcttcgccgc cttgccccgc
     4261 cggaggagac cccgcagcag cgactcgaca tcgcccgccg ggtgatgaag catctgccgg
     4321 aaacgcaatg gctgcgccac aaccgcaact tcgacgatca cgtcaacggc ggcgatgcgg
     4381 gcctttacga cttgctgctc aacccccgcg accgtgccta caccgttcct gaacttgccg
     4441 tgctgctgcg ggacgccggc ctgcgtgtca cttgctgggt cgaacccatc cgctacgatc
     4501 cggccccttt gctgcccgat cccaggcta
//
LOCUS       DASWQN010000587         3666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1507967, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000587 DASWQN010000000
VERSION     DASWQN010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507967"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VGC82_09860"
     CDS             <1..233
                     /locus_tag="VGC82_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423614.1"
                     /translation="AVYMAELFPTHVRATAVSFCNGSGRIVTSFGPLVAGLLVIPFGS
                     FNNAAATMTCFALLSMLAMWFGRETRHDELPR"
     gene            245..1018
                     /locus_tag="VGC82_09865"
     CDS             245..1018
                     /locus_tag="VGC82_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0423615.1"
                     /translation="MMFDKKITVHGIDLEVVRRGAGPPIVLLHGFQQVDPAARFMDLL
                     SQRAEVIAPSHPGFGRSARPKDFESVYDILNLYTTLLEALPHDKITLIGSSFGGWLAA
                     ELAVRAYRKIERLVLVDTVGIKISDRETPDITDVFNTHPDEVTRRTWHDPANAPDFNE
                     MSDEALVMRHRNWESLSLYAWHPYMHNPRLAYWLPRIDMPAQVIWGASDRIVTPIYGQ
                     AFAGLIPGAGFSVIDKAGHHPEIEQPDLFVDHVATFLNR"
     gene            1031..2281
                     /locus_tag="VGC82_09870"
     CDS             1031..2281
                     /locus_tag="VGC82_09870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0423616.1"
                     /translation="MDAWWQCEIPYPFVDKKITDAADSVRASLPNRLCDPRIAATLFD
                     EVLDEFLLCDDLGMNVIAIEHHAGINSLIGANPMITSILARQSRKVRVLSLGTLISLR
                     PDPVRVAEEYATADILSKGRFDIGFVKSGGTEIASSNTNPVNNEERFWEAIDLVKKSL
                     TTHDGPFSWEGKHFSHRHVNIWPRPYQDPHPPMWVATGDPRSAAEVGRRGMRHVLVLR
                     GPEGTKRAFDAHRQARAEAGLPKVTTDNFAYAALVYVGDTEQEGIEVGSKLLWFLNTS
                     LKSAPQFNKFLPGAAPPAAAPDLYRTKPKAEAAPALQDPALQDLALQDAEKGVAPASR
                     NAEKLISLSAPDAMRQGILFAGNPDSVYRQIMEFYDKVGGFGHLALVGRSGYMTHAES
                     SKSIRLFTQEVLPRLRAIKPMEVG"
     gene            2453..>3666
                     /locus_tag="VGC82_09875"
     CDS             2453..>3666
                     /locus_tag="VGC82_09875"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015074993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY0423617.1"
                     /translation="MRSIPIGIDATGQRTETDSMGAVQVPADRYWGAQTQRSLHHFSI
                     GDDRMPKELSRAYGIVKKAAAEVNAAEGRLAPDKAEAIVAAADEVISGALDEHFPLYV
                     WQTGSGTQSNMNLNEVISNRAIQLLGGTIGSKRPVHPNDDVNMCQSSNDTFPTAMHIA
                     TVRMLDDLLLPQLDALAQAIEAKADAWMDVVKIGRTHLEDAVPLTVGQEWSGYAHQLR
                     DAYRDIEASRRGLYELAAGGTAVGTGITAPKGFSAAIATRIATLTGQPFITAPNKFAA
                     QGALDAMTRTSAALRGLAVALMKIANDMRWLASGPRCGLGELLLPDNEPGSSIMPGKV
                     NPTQCEAIVMICIQVIGEDTAVAFAGSQGNFELNTMRPIIINNVLHSARILADGMEKF
                     RLHSVAGTQLNE"
BASE COUNT          718 a         1137 c         1165 g          646 t
ORIGIN      
        1 acgcggtcta tatggcggag ttgttcccga cccatgtgcg agcgaccgcg gtgtcgttct
       61 gcaatggctc cggccgcatc gtcaccagct ttgggccgct ggtcgctggg ttgctggtga
      121 ttccgttcgg cagcttcaac aacgccgccg ccacgatgac ctgcttcgcc ttgctcagca
      181 tgctggccat gtggttcggc cgcgagaccc gccacgacga actgccccgg taagggaacg
      241 aagtatgatg ttcgacaaaa aaattactgt tcatgggatc gatctggagg tcgtccgacg
      301 cggtgccgga ccacctatcg tgctgctgca cggcttccag caggttgatc ccgccgcccg
      361 cttcatggac ctgttgtcgc agcgggccga ggtcatcgcg ccttcgcatc ccgggttcgg
      421 acgatcggca cggccaaagg atttcgagag cgtttacgat atcctgaacc tctacacgac
      481 gctgctggaa gcgttgcccc acgacaaaat aacgctgatc gggtcttcgt tcggtggctg
      541 gctggctgcc gaactcgccg tccgcgcata ccgaaagatc gagcggctcg tgctggtaga
      601 taccgtcggg atcaagatca gcgaccgcga gacgccggat atcaccgacg tcttcaacac
      661 ccacccggat gaggtgacgc gccggacctg gcacgatccg gccaatgcgc ccgatttcaa
      721 tgaaatgtcg gatgaggcgc tggtgatgcg ccaccgcaac tgggagtcgc tgtcgctgta
      781 tgcgtggcat ccgtatatgc ataaccccag gctggcctat tggctgccac ggatcgacat
      841 gcccgcgcag gtaatctggg gagcatccga ccggatcgtg acgccgatat atggccaggc
      901 gttcgccggc ctgatacccg gcgccggatt ttcagtgatc gacaaggccg ggcaccatcc
      961 ggagatcgag cagccggacc tctttgtcga tcacgtcgcc accttcctga accgctgagg
     1021 aaaccgatcc atggatgcct ggtggcagtg tgaaatcccc tatccgttcg ttgacaagaa
     1081 gatcaccgat gcggccgatt ccgtgcgggc gtcgctgccg aaccggctgt gcgatccgcg
     1141 tattgcggcg acgctgttcg acgaggtgct ggacgagttc ctgctgtgcg acgacctcgg
     1201 catgaatgtc atcgccatcg agcaccacgc cgggatcaac tcgctgatcg gcgccaatcc
     1261 gatgatcacc agcatcctcg cgcgacaatc taggaaagtg cgggtactaa gcctcgggac
     1321 gttgatttcg ctgcgtcccg atccggtgcg ggtcgccgag gaatacgcca ccgcggacat
     1381 tctctcgaaa ggccgcttcg atatcgggtt cgtcaagtcg ggcggcactg aaatcgcgtc
     1441 gtccaacacc aatccggtga acaacgagga gcgattctgg gaggcgatcg acctggtcaa
     1501 gaagtcgctg accacgcatg acggcccgtt cagctgggaa ggcaagcatt tcagccatcg
     1561 tcatgtgaac atctggccga ggccatacca ggacccgcat ccaccgatgt gggtggcgac
     1621 cggcgatccg cgaagcgccg ccgaggtcgg gcgacgtggt atgcggcatg tgctggtcct
     1681 gcgcggccca gaaggcacga agcgcgcctt cgacgcgcac cgccaggctc gcgccgaggc
     1741 agggctgccg aaggtgacga ccgacaattt cgcatacgcc gctctggttt acgtgggcga
     1801 caccgagcaa gaaggcatcg aagtcggcag caagctgctg tggttcctga acaccagcct
     1861 gaaatcggca ccgcagttca acaagttcct gcccggtgcg gcacctccgg cggcggcgcc
     1921 cgatctttac cggaccaagc cgaaagccga ggccgccccg gcgttgcagg acccggcgtt
     1981 gcaggacctg gcgttgcagg atgcggagaa gggcgtcgca ccggccagtc gcaatgcgga
     2041 gaaactgatc tcactttcgg ccccggatgc catgcggcag ggcatcctgt tcgccggcaa
     2101 ccccgacagc gtgtatcggc agatcatgga attctacgac aaggtcggcg gcttcgggca
     2161 cctcgcgctg gtcggccggt cgggctacat gacgcatgcg gaatcgagca agagcatccg
     2221 gctgttcacc caggaggtgc tgccgcggtt gcgggcgatc aagccgatgg aggtggggta
     2281 gcgggacctc aacctttcgg cgcgcatgct gttcatgtgt ccggtctcgg gatggtggga
     2341 tcgtgattcc aaagagggac tcagcgcgcc ctgacatcaa tccgtgcttg agtgcctgaa
     2401 ccgcgacctg gggctgccgt tcgagccatg ggcaaccctt caggagcaag ccatgcgcag
     2461 cattccaatc ggcatcgatg ccacgggtca gcggaccgag accgactcca tgggcgcggt
     2521 gcaggtgccg gccgatcgct actggggcgc ccaaacgcag cgcagcctgc accatttctc
     2581 gatcggcgac gaccggatgc cgaaagaatt gtcgcgtgcc tatggcatcg tcaaaaaggc
     2641 tgcggccgaa gtgaatgcgg ccgaaggacg actggcaccg gacaaggcgg aagccattgt
     2701 cgcggcggcc gacgaggtca tctccggcgc gctggacgag cactttccgc tgtatgtctg
     2761 gcagaccgga tcaggcacgc aatcgaacat gaacctgaac gaggtcatct cgaaccgagc
     2821 catccagctc ctgggcggca cgattgggag caagaggccg gtgcatccga acgacgacgt
     2881 caatatgtgc cagtcaagta acgacacttt tccgaccgcg atgcatatcg cgaccgtccg
     2941 gatgctggac gatctcctgc tgccccaact cgatgccctg gcccaggcga tcgaggcgaa
     3001 agccgacgcg tggatggacg tcgtcaaaat cggccgcacc cacctggagg atgcggtgcc
     3061 gctgaccgtc ggccaggagt ggtcgggcta cgcccaccag ttgcgcgatg cctaccggga
     3121 tatcgaggca agccggcggg gactgtacga attggcggcc ggaggcacgg cggttggcac
     3181 tggcatcacc gcgccaaaag gcttcagcgc agccattgcg acccggatcg ccacgctgac
     3241 gggccagccc ttcatcaccg cgccgaacaa attcgctgct cagggggcgc tcgacgcgat
     3301 gactcgcacg tccgccgcgt tgcgtgggct ggcggtcgcg ctgatgaaga tcgccaacga
     3361 catgcgttgg ttggcatcgg ggccgcggtg cggcctgggc gaactgctgt tgccggataa
     3421 cgaacccggt tcttcaatca tgccggggaa agtcaatccg acgcagtgcg aggcgatcgt
     3481 gatgatctgc atccaggtga tcggcgaaga caccgcagtc gcgttcgctg gcagccaggg
     3541 caacttcgaa ctcaacacga tgcgccccat catcatcaac aacgtgctcc acagtgctcg
     3601 gattttggcc gacggaatgg agaaattccg ccttcactcg gtcgccggca cgcaactgaa
     3661 cgagaa
//
LOCUS       DASWQN010000588         1841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_189240, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000588 DASWQN010000000
VERSION     DASWQN010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189240"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VGC82_09880"
     CDS             complement(<1..434)
                     /locus_tag="VGC82_09880"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0423618.1"
                     /translation="MPLSRASREQRYDDYMATSHRPSFDGLRGLGFLLVVTTHVPSVP
                     LFGFLQGWTAVWIFLAMSGYLVTMLMVREEKRAGRIAFGPFLVRRFFRIVPSYWAAIL
                     IYWLACYTLPPLQDDYAGFMTRLPSYLAFVPEYADTDGFSIFT"
     gene            complement(595..1128)
                     /locus_tag="VGC82_09885"
     CDS             complement(595..1128)
                     /locus_tag="VGC82_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0423619.1"
                     /translation="MSTRNSLLIAVSALALISAVAVAAGGPNLGRVATPEEMAAWDVS
                     IGPEGVGLPPGSGTPKQGETVYLAKCVACHGEKGAGKPNDQLVGGQGTLSGDKPPVKT
                     VGSFWPYATTIFDYVRRAMPYNESKSLTNDEVYAVVAYILQLNGVVGENDTIDARTLP
                     KVKMPNRDGFVTFARGK"
     gene            complement(1115..>1841)
                     /locus_tag="VGC82_09890"
     CDS             complement(1115..>1841)
                     /locus_tag="VGC82_09890"
                     /inference="COORDINATES: protein motif:HMM:NF015369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0423620.1"
                     /translation="WTGVKLAILLQEAGVDPKAKWILAEGADAAGMSRSIPMEKVMDD
                     ALVALYQNGEHVRPANGYPLRLLLPGYEGNMNVKWLRRIKATESPTMTKDETSKYTIT
                     LPDGKSLQFVLPQEGKSVITRPSPGLIMKEPGLYEISGLAWSGYGKINKVEVSADGGK
                     SWAAAALQQPVLPKALTRFRMAWEWNGGPAILQSRAIDDTGYVQPTREQMIAKRGTRT
                     IFHSNAITTWSISDKGEVKHVYA"
BASE COUNT          350 a          587 c          529 g          375 t
ORIGIN      
        1 gtgaaaatgc tgaacccgtc ggtgtccgcg tattccggca caaaggcgag ataggagggc
       61 agccgcgtca tgaaccctgc atagtcgtcc tgcagcggtg gcagcgtata gcacgccaac
      121 cagtagatca ggatcgccgc ccagtagctc ggtacgatgc ggaagaagcg cctgaccagg
      181 aacggcccga acgcgatccg cccggcgcgc ttctcctccc gcaccatcag catggtcacc
      241 agataaccgc tcatcgccag gaatatccag accgccgtcc atccctgcag gaagccgaac
      301 agcggcacgc tcgggacatg ggtcgtgacg acgagcagga agccaaggcc acgcagtccg
      361 tcgaacgacg gacgatggct ggtcgccatg tagtcatcgt agcgctgttc gcgggaagca
      421 cggctcaatg gcattgtctg ggtccgcccg ccacgatcat acatggaggg gcggcatcgc
      481 gcatgggcgc gcgcctccgg cccgaaccgc tgatcggtgt ccggctcaag ggtcttttgg
      541 gacttctatg ctccgtgcca aaaatcgaac tgacattcgg gcagtctcac aaagctattt
      601 cccccgggca aatgtcacaa atccgtcccg gttcggcatc ttcaccttcg gcagcgttct
      661 tgcgtcaatc gtatcgttct cgccgaccac cccgttgagc tgcaagatat atgcgaccac
      721 agcatagacc tcgtcgttgg tcagcgattt cgattcgttg tagggcatcg cgcgccgaac
      781 gtaatcgaag atcgtcgtgg catagggcca gaagcttccg accgttttca ctggaggttt
      841 gtcgccggaa agtgtgcctt gcccgccaac aagctgatcg ttcggtttgc cggccccctt
      901 ctcgccatga caggcgacac atttcgcgag gtagacggtt tcgccctgtt tcggcgtccc
      961 actgcccggc ggcagcccga cgccctccgg accaatgcta acatcccatg cggccatttc
     1021 ttcgggcgtc gcgactcgtc cgaggtttgg gccgcccgcg gcgacggcca cggctgaaat
     1081 aagggcgagt gcggagaccg cgatgaggag actgttacgc gtagacatgt ttcacctccc
     1141 ctttatcgga aatgctccag gttgtgatcg cgttggagtg gaagatcgtc cgggtcccgc
     1201 gcttggcgat catctgctcg cgcgtcggct ggacgtaccc cgtgtcatcg atcgcgcggc
     1261 tttgcaggat ggccgggcca ccgttccatt cccacgccat gcggaaacgc gtcaacgctt
     1321 tcggcagcac cggctgctgc aacgcggccg ccgcccaact cttgccgccg tcggccgata
     1381 cctcgacctt gttgatcttg ccgtagcccg accaggcaag accagaaatc tcatagagcc
     1441 ccggctcttt cataatgaga cccggcgagg gacgcgtgat taccgacttg ccttcctgcg
     1501 gtaaaacgaa ttggagcgat ttcccgtcgg gcaaggtgat cgtatatttc gaggtctcgt
     1561 cctttgtcat cgtggggctc tcggtcgcct tgatgcgccg aagccatttg acgttcatgt
     1621 tgccttcata accgggcaac agcaaccgca gcggatagcc gttcgccggt cgcacatgct
     1681 cgccgttttg gtagagcgca accagcgcat catccattac tttttccatt gggatactgc
     1741 gactcatgcc agcggcgtcg gcgccttccg ccaaaatcca cttggccttc ggatcgacgc
     1801 cggcttcctg gagcaaaata gcgagcttca cgcccgtcca t
//
LOCUS       DASWQN010000589         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_980622, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000589 DASWQN010000000
VERSION     DASWQN010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980622"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..935
                     /locus_tag="VGC82_09895"
     CDS             99..935
                     /locus_tag="VGC82_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0423621.1"
                     /translation="MVSDGRTTFPLEESLVTNAKKADVSDALAKAFLPRDTFTVYYGP
                     LVINTGGKLVVIDTGNGPAAKVNSKGSAGQFTDNMVAAGFDPKAVDLVVISHFHGDHV
                     NGLLTADGTLAFPNAEVLVPATEWKFWMDDGEMSRASAGRMQGLFKNNRRIFDEGLKR
                     KVKPYEWGKEVAPGLLAVDTIGHTPGHTSYVLSSGSGKVFIQSDVTNNPNPFATHPDW
                     HLSFDQDADVAEKTRRRVYDMVVAEKLQLQGFHYPFPGLANLEKDGSGYRVIPAPWNP
                     AI"
     gene            1224..1931
                     /locus_tag="VGC82_09900"
     CDS             1224..1931
                     /locus_tag="VGC82_09900"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEY0423622.1"
                     /translation="MYTRPVFEPDRAASLAFAEARGFGVVCAHDGRKPVASSLPFCLE
                     YLSDGTPCVSFHVARGNALAGLAGGTSLGDPSVKSSWLLAVNGADAYVSPHWYASPDQ
                     VPTWLYQAVHLSGPVRLMTGQELAAHLDALSARFESWLAPKPPWTASEISAGRREAMM
                     QAIVGLVMTVEQIDGSFKLNQHKSDVDHVAVATALARQPEAAAQVLAGEMRALRPQLF
                     APQTTLLNAMAMPEGTM"
     gene            1931..>2137
                     /gene="argC"
                     /locus_tag="VGC82_09905"
     CDS             1931..>2137
                     /gene="argC"
                     /locus_tag="VGC82_09905"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644630.1"
                     /note="catalyzes the reduction of N-acetyl-5-glutamyl
                     phosphate to N-acetyl-L-glutamate 5-semialdehyde in
                     arginine biosynthesis and the reduction of
                     N-acetyl-gamma-aminoadipyl-phosphate to
                     N-acetyl-L-aminoadipate-semialdehyde in lysine
                     biosynthesis; involved in both the arginine and lysine
                     biosynthetic pathways; lysine is produced via the AAA
                     pathway, lysine from alpha-aminoadipate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY0423623.1"
                     /translation="MNAKKKIGILGASGYTGADAVRLLARHPDAEIVALTANMHAGKA
                     MNEVFPHFFMLDLPRLVEWEKVDWT"
BASE COUNT          417 a          672 c          658 g          390 t
ORIGIN      
        1 cgctgctgcc cacggcttcc gccaaggcgg ccgccccggt agccgacaag caggcgccaa
       61 gtttctatcg ctacaaggtt ggcgacatcc aagtcacggt ggtttccgac ggccgaacta
      121 cctttccgct ggaagagtcc ctcgtcacca acgccaagaa agcggatgtc agtgacgcgc
      181 tcgcgaaagc gttcctgccg cgtgacactt tcacggtgta ttacgggcca ctggtgatta
      241 ataccggcgg caagctcgtc gtgatcgaca ccggcaatgg ccccgctgcg aaggtcaaca
      301 gcaagggctc cgccgggcag ttcactgaca atatggtcgc cgccggcttt gatcccaagg
      361 cggtcgacct ggtcgtgatc tcgcatttcc acggcgacca tgtgaacggc ctgctgacgg
      421 ccgacggcac cctggcgttt ccgaacgccg aagtgctggt acccgcgacc gaatggaaat
      481 tctggatgga tgatggcgag atgagccgcg cgtcggcggg gcgcatgcag ggcctgttca
      541 agaacaaccg caggattttc gatgaaggtc tgaagcggaa agtcaaaccc tacgaatggg
      601 gcaaggaagt cgccccggga ctgctcgcgg ttgacacgat cggccatacg ccgggccaca
      661 cctcctatgt gctgtcatcc ggttcgggga aggtgttcat ccaatccgat gtgaccaaca
      721 atcccaatcc atttgcgacc catcccgact ggcacctgtc tttcgatcag gacgcggatg
      781 tcgcggaaaa gacgcgccgc agggtctatg acatggtggt tgccgagaag ctgcaactgc
      841 agggattcca ctatccgttc cccggactgg ccaatctcga aaaggatggc agcggctacc
      901 gggtcattcc agcaccatgg aatccggcga tctgaccgta ttctgcagac ccgcatcgat
      961 ggcaaagcca tcccccgttg ctccattgca tcgggagatg acggccagat accttgacga
     1021 aaaccggact tggccttata gccccccgca tgatcaacgc cgcgaccact ttcatcgctc
     1081 gccgccgccg cacaacagcg gtatgggcgg acgtctgcgt ttaagatatc agcctctgcc
     1141 gccggatccg gtccgcggca ttccccttca aagcagcgcg atctgatccg acggctcact
     1201 ggagcaagca ggagaagacc gccatgtaca cccgacccgt ctttgagccc gatcgagccg
     1261 cgagcctcgc cttcgcagag gcccgcggtt tcggcgtggt ctgcgcgcat gatgggcgca
     1321 agccggtcgc gtcctcgctg ccgttctgcc tcgaatatct cagcgacggc accccctgcg
     1381 tgtcgttcca tgtcgcgcgc ggcaacgcgc tggccggact cgccggcggc acgtcgttgg
     1441 gcgatccttc cgtgaaatcc tcctggctgc tcgcggtgaa cggcgccgat gcgtatgtat
     1501 cgccgcattg gtacgcctcg cccgaccagg tgccgacctg gctgtaccag gcggtgcatt
     1561 tgtcgggccc cgttcgcctc atgaccgggc aggaactggc cgctcatctc gatgcgctca
     1621 gcgcccgatt tgaaagctgg ctggcgccga agccgccgtg gacggcaagc gaaatatcgg
     1681 cgggacggcg cgaagcgatg atgcaagcga tcgtgggtct ggtgatgacg gtcgagcaga
     1741 tcgacggcag cttcaagctg aaccagcaca aatccgatgt cgaccacgtg gcggtcgcga
     1801 cggcgctggc gcggcaaccg gaagccgccg cacaggtttt ggcgggggaa atgcgtgcgc
     1861 tgcggccgca actgttcgcg ccgcagacga cgctattgaa cgctatggca atgcctgaag
     1921 gaacgatgtg atgaacgcaa agaagaagat cggcatcctc ggtgcgtccg gttacactgg
     1981 cgccgacgcg gtgcgcctgc tggcacggca tcctgatgcc gaaatcgtcg ctctcaccgc
     2041 caatatgcat gccggcaagg cgatgaatga ggtgttcccg catttcttca tgctggatct
     2101 gccgcggctc gtggaatggg aaaaggtcga ctggacc
//
LOCUS       DASWQN010000590         3233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2564853, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000590 DASWQN010000000
VERSION     DASWQN010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2564853"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VGC82_09910"
     CDS             <1..493
                     /locus_tag="VGC82_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0423624.1"
                     /translation="GNPDVVHIHGLSELPQVIAQMRQLGMPQQVTSYSAGYNPKLLAQ
                     LGPAAEGLIVTSLAPTGTTNPNVGKLLDLWKQVGRAPNGLPYLQYAWDSTVVVANLFA
                     WVDQNKLPVTGENLRKALLEIRTFDLPMTGKLVVTDDHRVRKPVYLYVVKDGQFTPLD
                     TIA"
     gene            515..1444
                     /locus_tag="VGC82_09915"
     CDS             515..1444
                     /locus_tag="VGC82_09915"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0423625.1"
                     /translation="MTDPVILLQILWTGIAMSSYFVLFTVAFALTLKVEKLWNFAQAG
                     LMGIAFYAMFWLLNAAGWPVWAGILASVLLTAGVTIGVEIWAIDVLRRRHSSGLIFFI
                     FTLIFSQFVAFLLTLLFGTEPETIFPSVMSPVRVVGGVAISDWDLQAIGLTLGMLTAL
                     YLLIRFTRDGQFMLAVADNEKLAEIYGISARRAYILSGVIATLLIVAGIWLYGTRAGV
                     TPDVPVGLILTAVIATLIGGLGRVFSAAIVAAGLALLQSFSILFIASKWQALLLYGVL
                     FFALLVFPRGIPAWRRRGIRPAPKPAAAAQAGG"
     gene            1448..2314
                     /locus_tag="VGC82_09920"
     CDS             1448..2314
                     /locus_tag="VGC82_09920"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0423626.1"
                     /translation="MEYLASTGVLIGVYIILATSYNLIIGFGGLNTIAHPIFFALGAY
                     TAALLGIETGLPALICIAAGIGVAVVASVALAFTTLRISGDYLLIASLGFQLGLLQTV
                     NNLEFTGGPAGLSNIPLTLGGPARGWLYLMISTVLAVLAIWLVQRSMAGPFGRAVRAM
                     RDDEVACASLGRSLFAMKVVIFAFGCGAAGLAGGIYAYYFQYISPEQFNVAQSATILT
                     MVVLGGIGTIWGPVIGAFVLVALPQRITFLELPSSLVGPLQGLIFTSLVIVFLFLRPH
                     GLVGSRMAGAAH"
     gene            2311..3078
                     /locus_tag="VGC82_09925"
     CDS             2311..3078
                     /locus_tag="VGC82_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0423627.1"
                     /translation="MSQEILRLENVCKRFGGIVVADDISLSLHAGDVLGLIGPNGAGK
                     TSLFNLISGLFRPDSGRIYLEGQLLTGPLHRRARAGIARTWQHMRLFRTMTVLENMMV
                     GSRDYPGESMLRSMAGTGSVRRAEATARDRAMHVLERMGLARTADINVVDLPFGQQKL
                     IGLARALMNDGRCLLLDEPMAGVEGSNYETMRRIVRDEAAAGRAVCVVEHNVSFIKDL
                     CTEGVFMSYGRILQRGSIEDLTRSRELTELYFGAVDA"
     gene            3071..>3233
                     /locus_tag="VGC82_09930"
     CDS             3071..>3233
                     /locus_tag="VGC82_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0423628.1"
                     /translation="MLEFRNVGVSYGGFIALRDISLSVQPGERVAIFGHNGAGKTTLL
                     RCGIGDVDDL"
BASE COUNT          513 a          994 c         1041 g          685 t
ORIGIN      
        1 tggcaacccg gacgtggtcc atatccatgg cctgtccgaa ctgccgcagg tgatcgcaca
       61 gatgcgtcag ttgggcatgc cgcagcaggt caccagttat tccgccggct acaatcctaa
      121 attgctggca cagcttggcc cggcggccga gggtctgatc gttacctcgc ttgcgcccac
      181 ggggacgacc aatccgaacg tgggaaagct cctcgacctg tggaagcagg ttggccgagc
      241 gccgaacggg ctgccgtatc tgcaatatgc ctgggattcc accgtggtgg tcgcgaacct
      301 gtttgcctgg gttgaccaga acaagctgcc ggtgacgggc gagaacctgc gcaaggcgct
      361 gctggagatc cggactttcg atctgccgat gactggcaag ctggtggtca cggacgacca
      421 ccgggtcagg aagccggttt atctgtacgt ggtgaaggac ggacagttca ccccgctcga
      481 caccatcgcc tgacccgcgc gactgccttc gacgatgacc gatcctgtca tcctgttgca
      541 gatcctgtgg accgggatcg caatgtcgtc gtacttcgta ctgtttacag tcgccttcgc
      601 gctgacgctg aaggtcgaaa agctctggaa cttcgcccag gccggactga tgggcatcgc
      661 gttctacgcc atgttctggt tgttgaatgc cgccggctgg ccagtctggg ctggcatcct
      721 cgcgtccgtc ctgctgactg caggcgttac cattggcgtg gaaatctggg cgatcgacgt
      781 gctgcgccgg cggcactcga gcgggctgat cttcttcatc tttacgctga tcttttccca
      841 gttcgtcgcc ttcctgctga ccctcctgtt tggcaccgaa ccagaaacaa tattcccgag
      901 cgtgatgtcg ccggtgcgcg ttgtcggcgg cgtagccatc agcgattggg acctgcaggc
      961 gatcggcctg acactgggga tgctaaccgc gctttacctc ctgatccgtt tcacgcgcga
     1021 cggtcagttc atgctggcgg tggccgacaa tgagaaactg gcagagatct acggcatcag
     1081 cgcgcggcgg gcctatatcc tgtccggggt catcgcgacc ctgctgatcg ttgccggtat
     1141 ctggctctat ggcacccgcg ccggcgtcac gccggatgtg ccggtcgggc tgatcctgac
     1201 ggcggtgatc gcgaccctga tcggcggcct cggccgcgtt ttctcggcgg ccatcgtggc
     1261 cgcgggactg gcattgctgc aaagcttcag cattctgttc atcgcctcga agtggcaggc
     1321 gctgctgctc tacggcgtgc tgttctttgc gttgctggtg ttcccgcgcg gcattccggc
     1381 atggcgccga cgcggcatca gaccggcgcc gaaaccagcg gcggccgcac aggctggagg
     1441 gtgagcaatg gaataccttg ctagtactgg tgtgctgatc ggcgtctaca tcatcctcgc
     1501 caccagctat aacctgatca tcggcttcgg cggcctcaat acgatcgcgc atccgatctt
     1561 cttcgcgctt ggagcttaca cggcggcgct tttgggcatt gaaaccggct tgccggcgct
     1621 gatctgcatc gcggccggca tcggcgtggc ggtcgtcgcc tcggtcgcgc tcgcgttcac
     1681 gacactccgc atctctggcg actatctgct gattgccagc ctcggctttc agctcggcct
     1741 gctgcagacc gtcaataatc tggaattcac cggcggcccg gccgggctat ctaacattcc
     1801 gctgacgctg ggcggcccgg cgcgcggctg gctgtatctg atgatcagca ccgttctcgc
     1861 cgtgctggca atctggctgg tccagcgcag catggccggc ccgttcggcc gcgccgtgcg
     1921 cgccatgcga gacgacgaag tggcctgtgc ctccctgggg cgcagcctgt ttgccatgaa
     1981 ggtggtgatc ttcgccttcg gctgtggcgc cgcgggcttg gccggtggga tctacgctta
     2041 ctacttccaa tacatcagcc cggaacagtt caacgtcgcg cagtcggcga cgatcctcac
     2101 gatggtcgtg ctaggcggca ttggcacgat ctggggaccg gtgatcggcg ccttcgtgct
     2161 ggtggcgcta ccccagagaa ttacgttcct ggaactgcca tcgagcctgg ttgggccgct
     2221 gcagggcttg attttcacct cattggtgat tgtcttcttg tttctgcggc cgcacggcct
     2281 ggtcgggtcg cgcatggcgg gagcggcaca ttgagccagg aaatccttcg actggagaat
     2341 gtctgcaagc gcttcggcgg cattgtcgtg gcggacgaca tctccctgtc gctgcacgcc
     2401 ggcgacgtgc tgggactgat tggtccgaac ggcgccggca agacatcttt gttcaacctg
     2461 atctccggcc tgttccggcc cgacagcgga cggatttatc tagaaggaca attgctgacc
     2521 ggtccgctgc atcgacgggc gcgcgccggc atcgcccgca cttggcagca catgcgtctg
     2581 ttccgcacca tgaccgtgct ggagaacatg atggtcggat cacgcgatta tcccggcgaa
     2641 tcgatgttgc gcagcatggc cggcaccgga tccgtccgcc gggcggaggc cacggcgcgc
     2701 gaccgggcga tgcacgttct ggaacgcatg gggcttgccc gcacggccga catcaacgtc
     2761 gtcgatctgc cgttcgggca gcagaaactg atcggcctgg cgcgggcgct gatgaatgac
     2821 gggcgctgcc tgctgctcga tgaaccgatg gccggggtgg aaggctccaa ctacgaaacc
     2881 atgcgccgga tcgtccggga cgaggccgcc gccgggcgtg cggtttgcgt tgtcgagcac
     2941 aatgtcagct tcatcaagga cctctgtacc gaaggggtgt tcatgtccta cggccggatt
     3001 ctgcagcgcg gctcgatcga ggacctgacg cgctcgcgtg aactgacgga actctatttc
     3061 ggtgccgtcg atgcttgagt ttcgcaacgt gggcgtcagc tacgggggct ttatcgcgtt
     3121 gcgcgacatc agcttgtccg tgcagccggg cgaacgcgtg gcgatcttcg gccataacgg
     3181 tgccggaaag acgacgctgc tgcgttgtgg catcggggat gtggacgacc tgt
//
LOCUS       DASWQN010000591         5863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_453249, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000591 DASWQN010000000
VERSION     DASWQN010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453249"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VGC82_09935"
     CDS             <1..434
                     /locus_tag="VGC82_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423629.1"
                     /translation="LGHQINTWRIHCRNLPSDSSDLGSTRSGAYSTTTWPLPISLGKN
                     VSPGQKGALRWVALVVLLAGRFMPPLDFHRQCHTVVDPSEPARGSSRTAAGGFGRRLR
                     LWGLPDHRWAAGRFEWTQIVLLASMTGFLASLDRGIAGTAA"
     gene            435..614
                     /locus_tag="VGC82_09940"
     CDS             435..614
                     /locus_tag="VGC82_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423630.1"
                     /translation="MVVARIVQGSAAAILPPQVFGSIRVLFQNERELARAMGAYAIMK
                     GMAVISQFSGGAPIQ"
     gene            905..1480
                     /locus_tag="VGC82_09945"
     CDS             905..1480
                     /locus_tag="VGC82_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HEY0423631.1"
                     /translation="MRTFLSTPTRRGYILLGSSLMLLSGCSDGLSRTFGLTRDAPDEY
                     TVTTRAPLSMPPDYNLRPPRPGAPRPQEQSERQQAEEALVPQLALGAPKADSSAGQAA
                     LIQEAGPAAPSDIRRTIDQEANRASSNEGFVDKLLYWRKPDTQQAQVDAQKESQRLRE
                     NAALGQSPDVGSTPIIQQRKEGWFTSLFSWI"
     gene            1516..2709
                     /locus_tag="VGC82_09950"
     CDS             1516..2709
                     /locus_tag="VGC82_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423632.1"
                     /translation="MPAETHFVQTGDAPILIGSGDETLSCRCGHTLIQGFAAVRFLAI
                     GIQCARCGEVTTTPPLPEGELPPRSAIVAAPSAEPRLTAMTVPPDVSVVGQSEMGRLQ
                     ALFQPATPDYTYRLTPALLDEAAAVFALHTGATLPDGEVIPGEPFAGLRDHALGWAVR
                     HLRWRMRSGAEGTGSQGVGDRGIAPWACLDDAPTANAVCHVTGFLHFVATWKRHPLFP
                     AMVATAADRGFSLHGLAPFAAAHCMTMMGNRINFPEPLGYPGRIEGFNLVAGLAETVG
                     VHVEVFDRFEFPFGEPWDAANLRAAIFEAVGAAQGRINLRHPGMLLLSPGTALAGYDE
                     ALIEAIKSAMQSLGRKNRGLLAAAPIVLRLQALADPQAVRFGYGLFPIANRHYHGSMP
                     VQVGA"
     gene            complement(2872..3336)
                     /locus_tag="VGC82_09955"
     CDS             complement(2872..3336)
                     /locus_tag="VGC82_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0423633.1"
                     /translation="MPVSPVPSGYSSVTPYLIIDGAARALDWYRQAFGARETMRLSGP
                     DGRVGHAEIEIGDSRIMLADEAPEHNAKAPEAFGGSPVSLHLYVPDVDKTMAKAVSIG
                     AEVKSQVEDKFYGDRMGSICDPFGHTWHITTHIEDVSVEEVERRMAEMAGKA"
     gene            3724..5415
                     /locus_tag="VGC82_09960"
     CDS             3724..5415
                     /locus_tag="VGC82_09960"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY0423634.1"
                     /translation="MTHRPTVYGTRHAISAGHYLAASAGFSILEAGGNAIDAGVAAGI
                     ALGVLQPDLVNFAGVAPILIRLADGTTESIAGLGWWPKGIQADLFMREHGGKIPDGVL
                     RTVIPAAPDAWITALRRHGTMRFAEVAASAIRFAADGFAVYPLLERSIAAHAEEYRRW
                     ESNTAIFLPNGRVPRTGEKFVQSDLARTIQYMADQDLAAGSDRLAGLDAAHHAFYRGD
                     IAQAIVRFQQQEGGYLSLSDLAEYSSAIEPVVRRSWRGHELLTCGAWCQGPALQQALA
                     LVEHVGIDGLAHNSADYLHRIVECLNLAMADREYHFGDPRFTDVPIDHLLDPATIARR
                     AAALRDDRAFGQMPAPLDRPNRLHAPTDADLPKVEADTSYCCVVDRWGNAFSATPSDG
                     SGNVPVVPGLGITPSGRGSQSRPDPRHPAGVAPGKRPRLTPNPAMLVTTAGGVMPLGT
                     PGGDVQIQAMLQVLLNVMHFGMDLQSAIEAPRVASYNFPSSFAPFEYFPGRLAVEGRI
                     DQATRNELAERGHTIQDWPDWTWLAGSVEAILTDPETGLIGGGADPRRPAYAIAI"
     gene            5541..>5863
                     /locus_tag="VGC82_09965"
     CDS             5541..>5863
                     /locus_tag="VGC82_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423635.1"
                     /translation="MDANFSAAAGGAAFSAATAHGQNTSTKRGNDGADIVGPSNKARE
                     AQQRFTVAPPRTDHGTLPNMKWSFADSHMRLEEGGWARETTVRELPVSKEMAGVNMRL
                     KTNVAR"
BASE COUNT         1031 a         1905 c         1883 g         1044 t
ORIGIN      
        1 aattgggcca ccaaatcaac acctggcgga tacattgtcg gaacctaccg tccgattcat
       61 ccgatctggg ttcgacgaga tcaggcgcct atagcacaac aacctggccc ttaccgatca
      121 gtttagggaa aaacgtatcg ccagggcaaa aaggtgccct ccgctgggtg gcgcttgtcg
      181 tgctgctggc gggccgcttc atgccgccgc tggattttca tcgtcaatgt cacactgtcg
      241 tcgatccatc agaacctgca cgcggctcca gccgaactgc agctggaggt tttggacgac
      301 gcctgcggct atggggtctt cctgatcacc ggtgggcggc tgggcgattt gaatggacgc
      361 agattgtgct tttggccagc atgaccggat tcctggccag cctggaccgc ggtatcgctg
      421 gcacggccgc ctgattggtc gtcgcccgga tcgtccaggg atcggcggca gccatcctgc
      481 cgccgcaggt gttcggctcc atccgcgtgc tgttccaaaa cgagcgtgaa cttgcacgcg
      541 ccatgggcgc ctacgccatc atgaagggca tggccgtgat cagccagttc agcggcggcg
      601 cgccgatcca gtgaagtccg gccggttggg acggcagcga tcttccagct gcccttgccg
      661 gttccggcgc ggcggtcagt atcgccacta tcgcatcctt atccttcatg atgctggaaa
      721 acggcaccag ccaatgccca accttctcgg ttgcgcagtg gccggttgcg cagtgggcgg
      781 cgacgggcgg gctgttcagg caatactgat cgccatgccg ccgcgtcgtt tgcaaacggg
      841 cgcaggcgcg gataatgccg ccgcatactt gcggcccaag ccagtgaatg agtatgagcg
      901 tcacatgcga accttcctgt ccaccccgac ccgacgcggc tacattttgc tgggaagcag
      961 cctgatgctg ctgagcggct gcagcgatgg cctgagccgt accttcggcc tgacgcggga
     1021 tgcgccggac gaatacacgg tcacgacccg ggcaccattg tcgatgccgc cggattacaa
     1081 cctgcgtccg ccccgcccag gcgcaccgcg cccgcaggaa caaagcgagc ggcagcaggc
     1141 ggaggaagct ttggtgccgc aactggcttt gggcgcacca aaggcggatt ccagcgccgg
     1201 ccaggcagcg ctgatacagg aggccggacc ggccgcaccc agcgacattc gccgcacgat
     1261 cgatcaggaa gcaaaccgag ccagctcgaa cgagggcttt gtcgacaagc tgctgtactg
     1321 gcgcaagccg gatacgcagc aagctcaggt cgatgcacag aaggaatcgc agcgcctgcg
     1381 ggaaaatgca gcgctgggcc aaagtccgga cgttggcagc accccgatca tccagcagcg
     1441 gaaagaaggc tggttcacca gcctgttctc ctggatctga aggcggcttc tcctgaatct
     1501 gaaggcggcg agggcatgcc ggctgaaacg catttcgttc agacaggcga cgcgccgatc
     1561 ctgatcgggt cgggcgatga aacgctgtcc tgccggtgcg gtcataccct gatccagggt
     1621 tttgccgcgg ttcggtttct cgcgatcggt atccagtgcg cgcgctgcgg tgaggttacc
     1681 accaccccac ccttgcccga gggtgaattg ccgccgcgtt cggcgatcgt cgccgcgccg
     1741 tcggcggagc cgcggctgac cgcgatgaca gtgccgccgg atgtttcggt agttggccag
     1801 tcggagatgg gccggctgca agcgctgttc cagccggcga cgccggacta cacctaccgc
     1861 ctgacgcctg ccctgctgga cgaagccgct gcggtgtttg cactccacac cggcgcgacc
     1921 ctgccggacg gggaagtcat cccgggcgaa ccgttcgccg gcctgcgtga ccatgccctt
     1981 ggctgggcgg tgcggcatct gcgctggcgg atgcggagcg gggctgaggg aaccgggagc
     2041 cagggtgttg gagaccgggg tatcgcgcct tgggcgtgcc tggacgatgc gccgaccgct
     2101 aacgccgtct gccacgtcac cgggttcctg cacttcgtcg caacttggaa gcgccacccc
     2161 ctattccccg cgatggtcgc gacggcggcc gatcgtggct tctcgctgca cgggctggca
     2221 cccttcgccg ccgcccactg catgacgatg atgggcaatc ggatcaactt cccagagccg
     2281 ttgggctatc ccggccgaat cgagggcttc aaccttgtcg ctggcctcgc cgagacggtt
     2341 ggtgtgcatg tcgaggtgtt cgaccggttc gaatttccgt tcggtgagcc atgggacgcc
     2401 gccaacctgc gtgccgccat attcgaggcg gtcggtgcgg cacagggccg gatcaacctg
     2461 cgtcatccgg gcatgctgct gctgtcaccc gggaccgcac tggccggata tgacgaggcg
     2521 ctgatcgagg ccatcaagtc cgcgatgcag agtctcgggc gcaagaaccg cggccttttg
     2581 gcggcggcgc ccatcgtgct gcggctccag gcgttggcgg atccgcaggc ggttcggttc
     2641 ggctacgggc tctttccgat agccaaccgc cattaccacg gctccatgcc ggtgcaggtg
     2701 ggtgcgtaga gccgggttgc tgtctcggat gagtgcgctc gctttgacgc catcatgacc
     2761 ggtgcataga gcgcacacca gtggagcggc gtgaaggccg cacggaacct ccgccgcgaa
     2821 cgggcgtgaa gcccgggccg gcatgaagcc cgggtcgtcc cacctgtcgc tttatgcctt
     2881 gccggccatc tccgccatcc ggcgttcgac ttcctcgacc gaaacgtcct cgatgtgcgt
     2941 ggtgatgtgc caggtatggc cgaagggatc gcatatcgat cccattcggt cgccatagaa
     3001 cttgtcctcc acctggctct tgacctctgc tccgatggag accgccttgg ccatcgtctt
     3061 gtccacgtcg ggaacatata gatgcaggct gacgggtgac ccaccaaacg cttccggtgc
     3121 ctttgcgtta tgctccggcg cttcatcggc cagcatgatg cggctgtcgc cgatttcgat
     3181 ttcggcgtgg ccgacccgtc cgtccggacc ggacagccgc atcgtctccc gcgccccgaa
     3241 cgcctgcctg taccaatcca gtgctcgggc cgctccgtcg ataatcaaat agggcgtaac
     3301 gctgctgtaa cctgatggga ccggcgatac aggcattggt cgtttctccc gcagatcgcc
     3361 caaaattgcc ttggccggat gacgctgtca agaacagagt cagaacttta taacacgctt
     3421 ctcagggtga tcgcacgctg ataccacagc gtagtcatta cgacctgcaa tcttaactca
     3481 aatctggccg cggcacgatc ttcgcccgcg gaaggtggcg acaagaagaa ctggaaccgc
     3541 cgatgaaccc ggcccgccac ctccatccac agttccgctt ttcgtaccgc gaggcagccg
     3601 gccaatctgg gctcgaacca gaatcgttca acagaattca attcggtgga tcgtccagcc
     3661 ggactggacc cgcgctggga tttgccgcac gcttgaccct tccagacccg gaggagacgg
     3721 ctcatgacgc atcgtcccac agtctatggc acacgccatg ccatttccgc cggccactac
     3781 ctcgcggctt cggccgggtt ctcgatcctg gaggccggcg gcaacgcgat cgacgccggc
     3841 gtcgcggcgg gcatcgcgct gggggtgctg caacccgatc tggtcaactt cgcgggcgtc
     3901 gcgcccatcc tcatccggct ggcggacggc acaaccgaga gtatcgccgg cctgggctgg
     3961 tggccgaagg gcatccaagc cgacctgttc atgcgcgagc atggaggcaa gatcccagac
     4021 ggcgtgctgc gcacggtaat accagcggcg ccggacgcct ggattaccgc gctgcgccgg
     4081 cacggaacga tgcgtttcgc tgaagtcgca gcatccgcca ttcgctttgc cgccgacggt
     4141 ttcgcggtct atccgctgtt ggaaaggtcc atcgctgcgc acgccgagga atatcgccgc
     4201 tgggaatcca acaccgcgat cttccttccg aacggacggg tgccccgcac tggcgaaaag
     4261 ttcgtccagt ccgacctcgc ccggacgatc cagtacatgg ccgaccagga cctggccgcg
     4321 ggttcggatc gattggccgg cctcgatgca gcgcaccacg ccttctaccg gggcgacatc
     4381 gcccaagcca tcgtgcgctt ccagcaacag gaaggcggct atctgtccct gagcgacctg
     4441 gcggaataca gctccgccat cgaaccggtg gttcgccggt cctggcgcgg gcatgagctg
     4501 ctgacctgcg gcgcctggtg ccaaggaccc gcactgcaac aggccctggc gctggtggag
     4561 catgtcggca tcgatgggct ggcgcataac tccgcggatt acctgcaccg gatcgttgaa
     4621 tgcctgaacc tggcgatggc ggatcgggaa tatcattttg gcgaccctcg tttcactgat
     4681 gttccgatcg accatttgct tgaccccgcg acgatcgctc gccgagcggc ggctttgcgc
     4741 gacgaccggg cgttcgggca gatgccggca ccgctggatc ggccgaaccg gctgcatgcg
     4801 ccgaccgacg ccgacctgcc gaaggtggaa gccgacacct cctattgctg cgtcgtcgat
     4861 cgctggggca acgccttcag tgccacgcct tcggacggat cgggcaatgt tcccgtggtg
     4921 cccgggctcg gtatcacgcc gtccggccga ggctcccaga gccgtcccga tccgcgccat
     4981 ccggccgggg tggcgccggg caaacggccg cggctgacgc cgaaccccgc gatgctggtc
     5041 accacggctg gtggggtgat gccattgggg actcccggcg gcgacgtgca gatccaggca
     5101 atgttgcagg tgctgctgaa cgtgatgcac ttcggcatgg acctgcagag cgcgatcgag
     5161 gcgccgcggg tggcatcgta taatttcccg tcgtcgttcg caccgttcga atatttcccg
     5221 ggacgccttg ctgtcgaggg gcggatcgac caggcgacgc ggaacgaact ggcggagcga
     5281 gggcacacga tccaggactg gccggactgg acctggctgg caggatcggt cgaggcaatc
     5341 ctgaccgacc cggaaacggg gttgatcggc ggcggggcgg atccgcgacg gccggcttac
     5401 gcaatcgcca tttgaaccgc gaacgctgat gccggcggcg ctgaggtcca cgcccttccc
     5461 accaattggt gaagacagca tctgcaacac gctcctgcac ccgacgttgg cgaactacct
     5521 ggggtaatct ctgaggagta atggatgcaa atttttccgc cgcggctgga ggcgccgcgt
     5581 tctccgccgc caccgcgcac ggccagaaca cgtccacaaa acggggaaac gacggggcgg
     5641 atatcgtcgg tccaagcaac aaggcgcgcg aggcgcagca acggttcacc gtggcgccgc
     5701 cgaggacgga ccatggcacg ctgccgaaca tgaaatggtc gttcgccgac agccacatgc
     5761 gcctggaaga aggtggctgg gcacgtgaga ccacagtgcg cgaactcccg gtctccaagg
     5821 agatggccgg cgtcaacatg cggctgaaga ccaacgtcgc gcg
//
LOCUS       DASWQN010000592         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1905184, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000592 DASWQN010000000
VERSION     DASWQN010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905184"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VGC82_09970"
     CDS             complement(<1..473)
                     /locus_tag="VGC82_09970"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0423636.1"
                     /translation="MSTEKHNNKTAGEEHMDSATENFDAVVIGAGFSGMHMLKSLRDK
                     LGLRVRVYEAGETVGGTWYWNRYPGARCDSDAYIYCFTWDKQLLQEWEWSERYPEQPE
                     ILRYLEHVAKRHDLKRDMQFNTRVTGAEFDETSNLWTVHTDKGEDVTARDLIAAVG"
     gene            complement(605..1624)
                     /locus_tag="VGC82_09975"
     CDS             complement(605..1624)
                     /locus_tag="VGC82_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY0423637.1"
                     /translation="MIDRMPPPWKGLQTGRMPGFISYSQIERMVASMLDDVTAWQPDA
                     VVAIVRGGLVPATMVCCMLSLPLFMVAWDRETSITSWVGSAANVKRLLVVDDCCATGR
                     TMLSVESALRHQEYECATLTVVHDPETTGYLPNFSHPMTELFRFPWERGEATPAFRRL
                     RAMGAPADPSAERPFYGLDLDGVFLADIDRREYVTDLVGALRRRHALQLSDVLPAFPR
                     ERAIVITGRPEDDRALTEAWLARWGFQDLTVECRPHDVADDVNSVARYKAVTATRWGC
                     THFIESDPEQAIRIAAAAPHLVVSWWSAEEIRAWTIGAAAYSDEHRRPHEWLAALGGE
                     RDKIV"
     gene            complement(1646..>1954)
                     /locus_tag="VGC82_09980"
     CDS             complement(1646..>1954)
                     /locus_tag="VGC82_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0423638.1"
                     /translation="ASIEGWYAYLYGDPMPGNRMIRQANPDMSADLIDFARNTMIEHG
                     TVDSGDTKTSGIGAMTDVRWKSFYQSMTEVGVYKPGIDISKAYTTQFVNKRVGMPPER
                     "
BASE COUNT          380 a          619 c          565 g          390 t
ORIGIN      
        1 cccacggccg cgatcaggtc tcgcgcggtg acgtcttcgc ccttgtcggt gtgcaccgtc
       61 caaaggttgc tggtctcgtc aaactcggcg ccggtcacgc gggtgttgaa ctgcatgtcg
      121 cgcttcaggt catggcgttt ggccacatgc tcgagatacc gcaggatctc gggctgctcg
      181 ggatagcgtt cggaccattc ccattcctga agcagttgct tgtcccaggt aaagcagtag
      241 atataggcgt cagaatcaca acgtgcgccg ggatagcgat tccagtacca agtgccgccg
      301 acggtttccc cggcttcata gacgcgcacc cgcaggccca gtttgtcccg cagcgacttc
      361 agcatgtgca tgccggaaaa gcccgcgccg atcacgacag catcaaaatt ttctgttgcg
      421 ctatccatgt gttcctcacc agccgtttta ttgttgtgct tctctgtcga catagcgcgg
      481 cgtatttcgt gggctgccgc tttcggcgac ttcggcagct tgcagccgct ttatcggtca
      541 cgagaccgaa ggatagcgca cgatacgacc gccgcagcac agtacgaaga actcgtaggc
      601 cggttcacac gatcttgtcg cgttcgccgc ccaaggccgc gagccattcg tgcggccgcc
      661 ggtgttcgtc cgaataggcc gcagcgccga tcgtccaggc gcggatctcc tcagccgacc
      721 accaactgac caccaggtgc ggagccgccg ccgcgatgcg aatcgcctgc tccggatcgc
      781 tttcgatgaa atgcgtgcag ccccagcgcg tggccgtcac cgctttgtac cgtgcgacgg
      841 agttgacgtc gtccgccacg tcgtgcggac ggcattccac cgtcaggtcc tgaaaacccc
      901 agcgggcgag ccatgcttcg gtcagcgctc ggtcgtcttc cggacgaccg gtgatgacaa
      961 tggcgcgttc gcgtgggaac gcgggcaata catcggatag ctgcaacgcg tgccgacgcc
     1021 gtagcgcgcc aaccagatcg gtcacgtact cccggcgatc gatgtcggcc aggaacacgc
     1081 cgtccaaatc cagcccgtaa aacggacgtt cggcggaagg atcagccggt gcacccatgg
     1141 cgcgcaaacg gcgaaaagcg ggcgttgcct ccccacgctc ccaggggaag cgaaacaact
     1201 ccgtcattgg gtgcgagaag ttgggcaagt agccggtcgt ctccggatcg tgcacgaccg
     1261 tcagcgtcgc acactcatat tcctgatgcc gtagtgccga ttcaacggaa agcatcgtcc
     1321 gtccggtcgc gcagcaatcg tccacgacca gcaggcgttt cacgttcgcc gccgaaccca
     1381 cccaacttgt gatgctggtt tcccgatccc aagccaccat gaacaatggc aacgagagca
     1441 tgcagcacac catcgtggcc ggcacgaggc ctccacgtac aatcgccacg acggcatcag
     1501 gctgccaagc cgtgacgtcg tcgagcattg aggccaccat gcgctctatc tggctgtacg
     1561 agatgaatcc tggcattcgg ccggtctgca gacctttcca gggaggtggc atgcggtcga
     1621 tcataactac ttccaggtcg ccgggttaac gttccggagg catcccgacg cgcttgttca
     1681 cgaactgcgt ggtataggcc ttggagatgt cgatccctgg tttgtaaacc ccgacttcag
     1741 tcatggactg atagaaggat ttccagcgca catcggtcat cgcgccgatg cccgatgtct
     1801 tagtatctcc ggaatcgaca gtgccatgct cgatcattgt gttgcgagcg aaatcgatta
     1861 aatcggccga catgtcggga ttggcctgcc tgatcatcct gttccccggc atcgggtcac
     1921 cgtagaggta agcataccag ccttcgatcg atgc
//
LOCUS       DASWQN010000593         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_453282, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000593 DASWQN010000000
VERSION     DASWQN010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453282"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VGC82_09985"
     CDS             complement(<1..1326)
                     /locus_tag="VGC82_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY0423639.1"
                     /translation="MTQAAIARDLSSMLQPHSIALIGATDRSTWSKATFTNLTTRKYD
                     GQVHLIARRGGTVHGRQAATSCVTLGEKIDLGLLMVPAAGIEEAMADLAAAGARNAVI
                     LSSGFAETGQEGAESQEKLAALARRYNISLLGPNCLGFVNFLDDVPLWTGSLRSPTDP
                     GPIAIISQSGATAGFIAALAQQQEIGLSHLISTGNEADLDGATFANHLLDDPRVRTVA
                     MFIESVRIPYNFVAAAQKARALGKAIVALKIGLSDVTARSAQAHTGSLIGDDRVFEGI
                     CRQFGVVRVTSIEDLLFTADIIARTGRISGKGLGLVSVSGGACEIAADRLQVESVPLP
                     PLPDATAQALTEVLPSYGTPHNPLDITGAAVLEPELFEKALKIMGNDPSFSALACLFE
                     VPGVEAQASEFQLAALRHIGVGLRDQPLPAIMISHTLKPVTEVTRRIVAD"
     gene            1464..1880
                     /locus_tag="VGC82_09990"
     CDS             1464..1880
                     /locus_tag="VGC82_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEY0423640.1"
                     /translation="MSQTATKPVPVTDEISAPFFNGAREGKLMLQHCSGCDRWSFPVR
                     ERCPHCFAAKLEWRQASGRGTLYTFTIMHQVMNPGFASAVPYNVAQVDLEEGVRMVSN
                     VVGIRNDALRIGMKLEAVFEDVGVDISLPKFCPVAG"
     gene            1896..>2578
                     /locus_tag="VGC82_09995"
     CDS             1896..>2578
                     /locus_tag="VGC82_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY0423641.1"
                     /translation="MASQAKGAIVGLGVTPMGRIYGRRAVDFAAEAIALALDDAGLTK
                     DDVDGLLINANISGDMDVRSQMVLGFENLTLVNVMSAYGSTAGSMMQYACMAIREGLV
                     KTVVLVYADAPLTPSRGAGQSYAGPHRFPLGGMTGLKAAYGDFGANPAYAMAARRHMS
                     LYGTTEDHFGTIAVGQRKWAQMKRPMTLEDHHNSRFIAEPLRLLDCCLVSNGAVAVIV
                     TSSEQARDLR"
BASE COUNT          452 a          677 c          964 g          485 t
ORIGIN      
        1 atcggcgacg atgcggcggg tgacctcggt gaccggcttt agggtgtggc taatcatgat
       61 ggccggcagg ggctgatcgc gcagacccac gccgatgtgg cgcaaggcgg cgagctggaa
      121 ttcggatgcc tgggcttcga cgcccgggac ttcgaacagg caggccaggg cagaaaagct
      181 gggatcgttg cccatgatct tgagggcttt ttcgaacagt tccggctcca ggacggcggc
      241 gccggtgatg tcgagcgggt tatgaggggt gccgtagctg gggagcacct cggtcagggc
      301 ctgggcggtg gcgtccggta agggcgggag cgggacgctt tcgacctgca ggcgatcggc
      361 agcaatctcg caggcaccgc cggatacgga aaccaggccc aagcccttgc cgctgatgcg
      421 tccggtgcgg gcaatgatgt cggcggtgaa aagcaagtcc tcgatcgagg tgacgcggac
      481 gacgccgaac tggcggcaga tgccctcgaa gactcggtcg tcaccgataa gggagcccgt
      541 gtgggcttgg gcagatcggg cggtcacgtc ggacaagccg attttcaggg cgacgatcgc
      601 cttgccgagg gcgcgggcct tttgcgccgc cgcgacgaag ttgtatggga tgcggaccga
      661 ttcaataaac atcgcgacgg tgcggacgcg cgggtcgtcc agcaggtggt tggcgaaggt
      721 ggcgccgtcg aggtcggcct cattaccggt ggagatgagg tggctgagac cgatttcctg
      781 ctgctgcgca agggcggcga tgaagccggc ggtggcgccg ctttgggaga tgatggcgat
      841 gggacccgga tcggtggggg agcgaaggga gccggtccag agcggcacgt cgtctaagaa
      901 gttgacaaag ccgaggcagt tggggccgag cagggagatg ttatagcggc gggcgagagc
      961 ggcgagtttc tcctggctct cggcgccttc ctggccggtt tcggcgaagc ctgaactcag
     1021 gattaccgcg ttgcgggcgc cggcggcggc gaggtcggcc atggcttcct caatgccagc
     1081 ggcggggacc atgagcaggc cgaggtcgat tttttcacca agggtgacac aggatgtggc
     1141 ggcttgacgg ccatggacgg tgccgcctcg gcgggcgatg aggtggacct gcccgtcgta
     1201 tttgcgcgtg gtgaggttgg tgaaggtggc tttcgaccag gtggaccggt cggtggcgcc
     1261 gatgagcgcg atggagtgcg gctgaagcat ggaggataga tcgcgggcaa tggcggcctg
     1321 ggtcattggt gcttcccctg ggtttcttgt cgtccggttc cctgacggga gcataggatg
     1381 atgcgggttt cggcgggaag cccggagacg ttgtcagggc gggcggtgat cgttagcttt
     1441 ttcagtcaga ggggacaacg ccgatgtcac agaccgcaac gaagccggtt cccgtaacgg
     1501 atgagatcag cgcgccgttc ttcaatggcg cgcgcgaggg caagctgatg ctgcaacatt
     1561 gctcaggctg cgaccggtgg agctttccgg tgcgggagcg ttgcccgcac tgttttgccg
     1621 ccaagctgga atggcggcag gcgagcgggc ggggcacgct gtacacattt actatcatgc
     1681 accaggtgat gaacccagga tttgcatccg ccgtgcctta caacgtggct caggtggatc
     1741 tggaggaagg cgtgcggatg gtgtccaacg ttgttggcat ccgtaacgac gcgctgcgga
     1801 ttggcatgaa gctggaggcg gtattcgagg acgttggagt ggacatcagc ctgccgaagt
     1861 tttgtcccgt cgccggttga gcaggagggg aagtcatggc cagtcaggca aaaggcgcga
     1921 ttgttggtct cggcgtaacg ccgatgggca ggatctatgg gcgccgggcg gtggatttcg
     1981 cagcggaggc gattgcgctg gcgctggatg acgcagggct gacgaaggac gatgtcgacg
     2041 ggctgctgat caatgcgaac atttcgggcg acatggatgt gcgcagccag atggtgctgg
     2101 ggttcgagaa cctgacgctg gtgaatgtca tgagcgccta tggctctacc gccggcagca
     2161 tgatgcagta tgcctgcatg gcgatccgcg aagggctggt gaagacggtt gtgctggtct
     2221 acgccgatgc gccgctgacg ccgagccggg gtgctggcca gtcctatgct gggccacacc
     2281 gctttccgct tggcggcatg acgggcttga aagccgccta cggcgatttc ggcgccaatc
     2341 ccgcttatgc catggccgct cggcggcata tgtcgcttta cggcacgacg gaggatcatt
     2401 tcggcaccat cgcggtagga cagcgaaaat gggcgcagat gaaacggccg atgacgctgg
     2461 aagaccatca caactcccgc ttcatcgccg aaccgctgcg gctgcttgat tgctgccttg
     2521 tctcgaacgg cgccgtcgcg gtgatcgtta cttcgtccga acaggcacgt gaccttcg
//
LOCUS       DASWQN010000594         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_980904, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000594 DASWQN010000000
VERSION     DASWQN010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980904"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VGC82_10000"
     CDS             <1..662
                     /locus_tag="VGC82_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423642.1"
                     /translation="VALLLPAVFDLTARAGTRDTFPTDERLSIGASAVLLLLYSANLV
                     FTLITHRDIFAGDESRATNGWSLAKSLGVLIGGTILVALQAEFVSEALTATASQLHVS
                     KAFLGVIVLALIGTSADLFAAVAFARQDRMSIVFSICIGSAIQMALVVAPLLVLLSWL
                     IGHPMNLVFDNPLDLFAIAGTAFVVRAIAADGETTWFEGLLLVGVYVLFGLAFFFVEP
                     G"
     gene            979..1449
                     /locus_tag="VGC82_10005"
     CDS             979..1449
                     /locus_tag="VGC82_10005"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY0423643.1"
                     /translation="MSSENPTAGRRSDFTFVSRLGPRTRDAMIAAAAEMALADGRAEP
                     AEHRGLLMFLKKNNLLFLLGRTETTKRYIAEIGRATERHAASSPPPTSSENRWHDLTV
                     RVRPIAGMESARLVAAAAACVAAADGVVHAGELELLRSLRDVLGLDDASTDARS"
     gene            1470..2072
                     /locus_tag="VGC82_10010"
     CDS             1470..2072
                     /locus_tag="VGC82_10010"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEY0423644.1"
                     /translation="MGSARAGTAEKLRRLMGRHRAGGVTLGAIIDDLGAAGMGLCLLL
                     FSLTSLIPGVAPVFGLALCALACGIVLGHGKPLLPERLRQWQMDRDRVDNGLRRLAPV
                     VAWMERWFRPRKSHLLRGPGIRVVGLASLVNGVLIVLPIPFGNTAPAIAMLILSLGMV
                     AGDGIAVGVGLLATVVALAMDVGMIVLGYTAFIAMIEHLF"
     gene            2093..2854
                     /locus_tag="VGC82_10015"
     CDS             2093..2854
                     /locus_tag="VGC82_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY0423645.1"
                     /translation="MDIQANLQQWANLQQWADPQLWASLLTLTAMEIVLGIDNLVLLA
                     VMADRLQEHQRASARRLGLLAALGTRLVLLLSITWIMRLTAPIIDLPWHSFSWRDLIL
                     IGGGLFLVYKGTVEIHHRVEEEDVDQAPRPGHASFAGTIAQIALLDIVFSLDSVITAV
                     GMANHLYVMIAAIVIAIGIMMLASGTVAAFISRHPTVKMLALSFLLLIGMTLIGDGFG
                     AHVPKGYIYAAMGFSALVEVLNQVVARARRRRRAV"
BASE COUNT          505 a         1025 c         1005 g          693 t
ORIGIN      
        1 tcgtcgcgct gctgctacct gccgttttcg atctcaccgc ccgggccgga acacgcgata
       61 cttttcccac cgatgaacgg cttagcatcg gagcttccgc cgtcctgctg ctgctctatt
      121 ccgccaatct cgtcttcacc ctgatcactc accgcgacat cttcgccggc gacgaatcac
      181 gcgcgaccaa cgggtggagc ctggcaaaat cgctgggcgt attgatcggc ggcacgatcc
      241 ttgtcgcgct acaggcggaa tttgtgtcgg aggcgctaac cgcgacggcc tcccagcttc
      301 atgtgtcgaa ggccttcctg ggggtaatcg tgctggccct gatcggcacc tcggccgact
      361 tgttcgccgc ggtcgctttc gcgcgtcagg atcgcatgag cattgtcttc agcatctgca
      421 tcggatcggc catccaaatg gcgctggtgg tggcaccgct gctcgtgctg ctgtcctggt
      481 tgatcggcca cccgatgaac ctggtcttcg acaatccgct cgatcttttc gccatcgcgg
      541 gaaccgcgtt cgtggtgcgc gccattgccg ccgacggtga aaccacgtgg ttcgaggggc
      601 tcctgcttgt cggcgtctat gtcctgttcg gcctcgcctt ctttttcgta gagccgggtt
      661 aaagcatgtc cagcctgacc gcgtaggctg gccatgctct ttctcttgtt ctggccgcac
      721 gcttcacacg ttccacccag catgatcatg ctccgggagc tgtggcttgg gtggatgact
      781 tgaaacccgc ctgaacgagc ctacgtcttc gtgcataaca cgatccgggc ccctctggcg
      841 ggacgtcggt gcaagccggc caaccttgcg atgtcggatc accgcgtgcg gtttcgcagc
      901 ggtcgagccc ggcccgtcgt tctccgtgtt cgatcctgct ccgcatgtgg ctttcgaaac
      961 atcgcacgga gcaagaccat gtcgtcagaa aaccccaccg ccggtcggcg tagcgatttt
     1021 accttcgtgt cccggctcgg gccgcgtacg cgcgatgcga tgatcgccgc ggcggcggaa
     1081 atggccctgg ctgatggccg cgccgaaccc gccgaacacc gcggcctgct catgttcctg
     1141 aagaagaaca atctgctgtt cttgctcggc cgcaccgaga cgaccaagcg ctacatcgcc
     1201 gagatcggcc gcgccactga acgccatgcg gcttcttcgc cgcctccgac aagcagcgaa
     1261 aaccggtggc atgatctgac tgtcagggtg cgcccgattg cgggaatgga gtcggccagg
     1321 ctggttgcag cggccgccgc ttgtgtggcc gccgccgatg gtgtcgtcca tgctggagaa
     1381 ctcgaactgc tgcgttcgct gcgcgatgtc cttgggctgg acgacgcttc caccgatgcc
     1441 cgctcatgag ccggtcagcg acactcccaa tgggctcagc gcgagcgggt acggctgaga
     1501 aactgcgtcg gctgatgggc agacaccggg ctggcggagt aaccctcggc gcgatcatcg
     1561 atgatctcgg ggctgccggt atgggcctgt gcctgctgct gttcagtctg acgtcgctga
     1621 tcccgggcgt cgccccggtg ttcggtctcg cgctgtgcgc gctcgcctgc ggcatcgtgt
     1681 tgggccatgg aaaaccgtta ttgccggagc ggctccgcca gtggcagatg gatcgggatc
     1741 gggtggacaa cggtttgcgc cggctcgcac cggttgtcgc ctggatggaa cgatggttcc
     1801 gccctcgcaa gagccacttg ctgcgcgggc caggtattcg tgtggtcggc ttggccagcc
     1861 tggtcaatgg cgtactgatc gtgctgccga ttccgttcgg caacaccgca cccgccattg
     1921 ccatgctgat cctgtcgctc ggtatggtcg ccggtgacgg catcgcggtc ggagtgggcc
     1981 tgctggcaac cgtcgtcgcc ctcgccatgg atgtcgggat gatcgtcctg ggttatacgg
     2041 ctttcatcgc catgatcgag cacctgttct gatatccgga gagtagaggc cgatggatat
     2101 ccaagccaac ctgcagcaat gggccaacct gcagcaatgg gccgacccgc agctttgggc
     2161 cagccttttg acgctcaccg ccatggaaat cgtgctcggt atcgacaatc tggtcctgct
     2221 ggctgtcatg gccgaccgcc tgcaggagca tcaacgcgct tccgcccgcc gcctgggact
     2281 gcttgccgcg ctcggcactc gcctggtctt gttgctcagc atcacctgga tcatgcggct
     2341 gacggcgccc atcatcgatc tgccatggca cagtttttcc tggcgcgacc tgatcctgat
     2401 cggcggtggc ctgttcctgg tctacaaagg caccgtggaa atccaccacc gtgtggaaga
     2461 ggaggacgtg gaccaagctc cacgtcccgg ccatgccagc tttgccggca ccattgcgca
     2521 gatcgcgttg ctggacattg tgttctcgct cgacagcgtc atcaccgcag tcggtatggc
     2581 caaccacctg tatgtcatga ttgccgcgat cgtcattgcc atcggcatca tgatgctcgc
     2641 atccggcacc gtcgcggcgt ttatcagccg gcacccgacg gtcaaaatgc tggcgttgag
     2701 tttcctgttg ctgatcggca tgaccctgat cggcgatggt ttcggggccc atgtgccgaa
     2761 aggctatatc tatgccgcca tgggtttctc ggcgctggtg gaggtgttaa atcaggtcgt
     2821 ggcccgggcc cgacgacgcc ggcgtgcggt atagcccgcg ccgtgagcac gcggtccatc
     2881 ctgctcgttg cgaaatttca ctgccggcat gacgcgaccg cttccaacga ggccaacagg
     2941 ctctggagcg gcaaggatcg atggcctcgg tggcagcgtg atcgcctttc ggaggatcgc
     3001 gtgacgcggc gtcgtccaat ggtcggaata gccgacctcc gccgcctgag attcagccag
     3061 cggcggaggt gtccgatcat cggcttcgga gtttagcgct cggtgcgctg tccgtccgag
     3121 tcagccgtcg tgtgcgtcga aacgcgacgg aagctttcct gcgccatgtg catggtgatc
     3181 tgcatgatgc gggaattgta gtccatccag gaccgggccg tgtaggca
//
LOCUS       DASWQN010000595         4464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1377580, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000595 DASWQN010000000
VERSION     DASWQN010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377580"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VGC82_10020"
     CDS             <1..258
                     /locus_tag="VGC82_10020"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0423646.1"
                     /translation="KLKPSEYWQRQCKATFQFDPIGPKLLNTNNLMTEDTLMWGSDYP
                     HTDGIWPESDKYIEEQFRGLTADQIHKITCENAAKFYNLTN"
     gene            complement(322..1317)
                     /locus_tag="VGC82_10025"
     CDS             complement(322..1317)
                     /locus_tag="VGC82_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0423647.1"
                     /translation="MPSRRFFMGAGAALSAAPILATTPAKAAAVNGEKVISLFRSLPG
                     RTAVKIWSPAAGTKPALDIGFNAGTRLFAGSAIKTFILGESLRQADSPVVVKTLTETQ
                     LTLDASVWSVDSVTFNPPNLTGTVTQRTALEATIMHSDNTATDMCIKHAGAENVRTFV
                     AGAKLRQTAIPDSTRSFFGYLLGAPDFRSFSWAQLQQVRSAPIVNPALNDVETLASSA
                     NDFVSYYSRALQGEFFQHKQTLNEFRRILSLGDAIWLLPLPLGVSAFVKGGSIDVPGF
                     HCVCVPGGMFFNNRWVYFAMTINWDAKEETDPKTVEAFVTATRKALTLVVNTLAA"
     gene            1537..2571
                     /locus_tag="VGC82_10030"
     CDS             1537..2571
                     /locus_tag="VGC82_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423648.1"
                     /translation="MPEQNLLERAPSPSGAPAPRLKPPVLFSHTQRLIGEIEALVEEP
                     LITYWNSASGSICDNDVLGLYGVLRDTRPAKRLSLFVKSDGGSGQASLRMINLLRQHV
                     EHLTVLVPLECASAGTMLALGADCIQMGPLAHLSAVDTSLTHELSPLDRDNHRVKVSQ
                     DELQRAARLFQGDRTQDNPFAALFQHVHPLVIGAVDRSSALSTRLCIEILGYHMTDAA
                     KAKAISDTLNSGYPSHSYPITLREARRIGLNAEALSEQLNRKLLELNAIYSEMGQRAT
                     TDFDDRNSHDNSIMNIIEGNGRQVFFQHEKDWHYRAEERRWITLNDRSSWRKAEWAGD
                     EVSVSVYPIR"
     gene            2725..>4464
                     /locus_tag="VGC82_10035"
     CDS             2725..>4464
                     /locus_tag="VGC82_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY0423649.1"
                     /translation="MNDAATDPTRDYRDTVFLPRTRFPMRGDLPKKEPQILAKWDAMD
                     LWGKIRASSAGRELFILHDGPPYANGNIHLGTALNKIHKDMINRSRQMAGYDANYIPG
                     WDCHGLPIEWKIEEEYRRKNKDKDSIPILDFRAECRAYAEHWLGVQTQEFRRLGVEGD
                     FANRYATMDRESESAIAAEICKFLLNGALYRGLRPVMWSPVEKTALAEAEIEYHDHTS
                     TTIWVRFPVVRASVPALEGAAVAIWTTTPWTIPGNRAVAAGATFDYAVVRVDEVAPAS
                     RARVGEKIVIAATLLESVAKDTGIEAYTVLTTVKGAHLAGTVMAHPLRGAKGANGGYD
                     QDTPLLLGDFVTTEAGTGFVHIAPGHGEDDFDLGRANGLDVPETVGDDGTFNPWVPLF
                     GGVHVYRAGEPVAAALIEAGNLLARGTIRHSYPHSWRSRAPLIFRATPQWFIRLDGPE
                     RIREKALAAIAATHFVPEQGRNRIGSMVAGRPDWCVSRQRAWGVPITVFVHKASGEAL
                     RDPAVVERITQAFEAEGADAWYASPPSRFLGPDYNPDDYEQVRDIVDVWFESGCTHAF
                     VLEKRGLPWPADLY"
BASE COUNT          861 a         1404 c         1402 g          797 t
ORIGIN      
        1 aagctgaagc catccgagta ttggcagcgc cagtgcaagg cgacgttcca gttcgatccg
       61 atcgggccga agctgttgaa caccaacaac ctgatgaccg aagacaccct gatgtggggg
      121 tccgattatc cgcacaccga cggcatctgg ccggagtcgg acaaatacat cgaagaacag
      181 ttccgcggcc tgaccgcaga ccagatccac aagatcacct gcgagaacgc cgcaaaattc
      241 tataacctga cgaactgagg ctgaatgccg gggagccctc tcgaccggga gagctgcccc
      301 ggccgccggc atcatcgcag atcaggcggc aagcgtgttc acgacgagcg tcaatgcctt
      361 gcgcgtcgcc gtgacgaacg cctcgaccgt cttcggatcg gtctcttcct tggcgtccca
      421 gttgatggtc atcgcgaagt aaacccaccg gttgttgaag aacatgccac cggggacgca
      481 cacgcagtgg aaacctggca cgtcgatgct gccgcccttg acgaacgcgc tgacaccaag
      541 cggcagggga agaagccaga tggcatcgcc gagggaaagg atccgcctga actcgttcaa
      601 tgtctgcttg tgctggaaga actcgccctg cagtgctctg gaatagtatg agacgaaatc
      661 attggccgac gatgccagcg tctcgacatc gttcaatgct ggattgacga tgggtgccga
      721 ccgaacctgc tgaagctgcg cccagctaaa gctcctgaag tccggggcac ccagcaagta
      781 gccgaagaag ctgcgggtac tgtccggaat agcggtctgc cgcagtttag cccctgcgac
      841 gaatgtccgc acgttctcgg cgccggcgtg cttgatgcac atgtcggtgg cggtgttgtc
      901 gctgtgcatg atcgtcgctt ccagcgcggt ccgctgcgtc accgttccgg tcaggttggg
      961 cggattgaac gtgacgctgt ccaccgacca gacgcttgca tcgagcgtga gctgggtctc
     1021 cgtcagtgtc ttcaccacaa cgggcgaatc agcctgccgc aacgactcgc caaggatgaa
     1081 cgtcttgatc gcgctgcccg caaacaggcg cgttccggca ttgaagccga tgtcgagcgc
     1141 cggtttggtg cctgcagcag gcgaccaaat cttgaccgcg gtccggccgg gcagcgatcg
     1201 gaacaaggaa atcacttttt ccccgttgac ggccgccgcc ttggccggag tggtcgcaag
     1261 aatcggagcc gcggaaagcg ccgcgccagc ccccatgaaa aatcggcggg acggcattct
     1321 ggtctctcct caagccgagg ttgacttgcg attgatcggc atccgaaaga gagcatatcc
     1381 gggtcagatc tgaaagcttc gctggagcgc ggttggcgga accagaatac gatgcaaata
     1441 agtaatcaag atcgacgcgg aaaaggctag gcttgggcgt ggttcgactg tacggtgcct
     1501 cacactactg tgaaatattt gtgggaaacc agtcgtatgc cggaacagaa tctactcgag
     1561 cgcgcgccgt caccctcggg tgcacccgca ccaaggttga aaccgccggt cctgttcagc
     1621 catacgcaac gcctgatcgg cgagattgaa gccctggtcg aggagccttt gatcacctac
     1681 tggaattccg ccagcggttc gatctgcgac aacgatgtcc tgggcctgta tggcgtcctg
     1741 cgggataccc gtccggccaa gcgactgtca ttgttcgtga aatccgacgg tgggtccggt
     1801 caggcctcgc tgcgcatgat caacctgttg cgccagcatg tcgaacacct gacggtcctt
     1861 gtgccgctgg aatgcgcttc tgccggaacg atgctggcgc tcggtgccga ctgcatccag
     1921 atggggccgc tcgcgcatct gtccgctgtc gatacctcgt tgacccacga actatcgccg
     1981 ctcgatcgcg acaaccaccg cgtcaaggtc agccaggatg aactgcagcg cgctgcccgg
     2041 ctgttccagg gcgaccgcac acaggacaat cctttcgcgg cgctgttcca gcatgtccat
     2101 ccgctggtga tcggcgccgt tgaccggtcc agcgccttgt ccacccggct ttgcatcgag
     2161 atcctcggct atcacatgac cgacgccgcg aaagccaagg cgatcagcga cacgctgaac
     2221 tccggctatc catcccacag ctatcccatc acgctgcggg aggctcggcg gatcggcctg
     2281 aacgccgaag cgctgagcga acagcttaac cgcaagctgc tggaattgaa cgcgatctat
     2341 tccgagatgg ggcagcgtgc gacgaccgac ttcgacgacc gcaattcgca cgacaacagc
     2401 atcatgaaca tcatcgaggg caacgggcgc caggtgttct tccagcacga gaaggactgg
     2461 cactaccgtg cggaagagcg ccgctggatc accctgaacg accgcagttc gtggcggaag
     2521 gctgagtggg cgggtgacga agtcagcgtt tcggtctacc cgatccggta gccacgccag
     2581 cgaacggcgt tccggtggtc aatctcacct ttccgcgttc agcatggtcg cggcggtcgc
     2641 ttccgtgtat aggctggccg ccctgaacgc ttcgtccggc tcgcaccggt cgttgccacc
     2701 tgcactttgc ccgacggaac cgccatgaac gacgccgcta ccgatcccac gcgcgattac
     2761 cgcgacactg tgtttctgcc gcggacccgc ttccccatgc gcggcgacct gccgaagaaa
     2821 gagccgcaga tcctggcgaa gtgggatgcc atggacctgt ggggcaaaat ccgggcgtcg
     2881 tcggctggcc gggaactgtt catcctgcat gacggcccgc cctatgcgaa cggtaacatc
     2941 cacctgggca cggcgctgaa caagatccac aaggacatga tcaaccgcag ccgccagatg
     3001 gccggctatg acgccaacta catccccggc tgggactgcc acggcctgcc gatcgagtgg
     3061 aagatcgagg aggaataccg ccgcaagaac aaggacaagg attccatccc gatcctcgac
     3121 tttcgtgcgg aatgccgggc gtatgcggag cattggctgg gcgtgcaaac gcaagagttc
     3181 cgccgcctcg gcgtcgaagg cgatttcgcc aatcgctatg ccaccatgga ccgggaatca
     3241 gagtcggcga tcgccgccga gatctgcaag ttcctgctga acggcgcgct gtaccgcggc
     3301 ctgcgcccgg tgatgtggtc cccggttgag aagaccgcgc tggccgaagc cgagatcgag
     3361 tatcacgacc acacttccac gacgatctgg gtgcgattcc cggtggtcag ggcatccgtg
     3421 ccggcgctgg agggtgccgc ggtggcaatc tggaccacca cgccctggac gataccggga
     3481 aaccgggcgg tcgccgccgg ggccacattc gattacgctg ttgtgcgggt ggatgaggtc
     3541 gcgccggcct cgcgtgcccg cgtcggggaa aagatcgtga tcgcggcgac gttgctggaa
     3601 tccgtggcga aggacaccgg catcgaggct tacaccgtcc tgacgacggt caagggggcg
     3661 caccttgccg gcaccgtgat ggcgcatccg ttgcgcggcg cgaaaggcgc caacggcggt
     3721 tacgaccagg atacgccgct gctgttgggg gacttcgtca ccaccgaagc gggcaccggc
     3781 ttcgtccaca tcgcgcctgg ccacggcgag gatgacttcg atcttggccg cgccaacggc
     3841 cttgatgttc cggaaacggt tggcgacgac gggacgttca atccgtgggt gccgctgttc
     3901 ggcggcgtgc atgtctatcg ggccggcgaa ccggtcgccg cggcactgat cgaagccggc
     3961 aacctgctcg cccgtggcac gatccggcac tcctatcccc attcatggcg atcgagggcg
     4021 ccgctgatct ttcgcgcgac gccgcaatgg ttcatccgcc tggacgggcc ggaacgcatc
     4081 cgcgaaaagg cccttgccgc catcgctgcg acccatttcg tgccggagca ggggcgcaac
     4141 cgcatcggct cgatggttgc cggccggccg gactggtgcg tcagccggca gcgcgcctgg
     4201 ggcgtgccga tcaccgtctt cgttcacaag gcctccggtg aggccctgcg cgatcccgcg
     4261 gtggtggagc ggattaccca ggcgtttgag gccgagggtg ccgatgcctg gtatgcctcg
     4321 ccgccgtcgc gctttttggg accggactac aacccggacg actacgagca ggttcgggat
     4381 atcgtcgatg tgtggttcga atcgggctgc acccacgcgt tcgtactgga gaagcgtggc
     4441 ctgccctggc cggccgacct gtat
//
LOCUS       DASWQN010000596         7277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2432698, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000596 DASWQN010000000
VERSION     DASWQN010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2432698"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VGC82_10040"
     CDS             complement(<1..731)
                     /locus_tag="VGC82_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423650.1"
                     /translation="MADDPLVPLSDTLIAGRFAVDSSQILPDAGGGLKAYLARDRLAS
                     DGKRIAIAVSRDASPRANALKALKDPIDNLMGPLGHGIAPLAGGKGEGYFIVCTPPPG
                     PSVSAPLNAWPEKALIDLVLRPVARVLEVLHSRKLTHRAIRPNNVFQSAPGQPVVLGA
                     AWAAPPAMHQPVVFECPYSAMCHPTGRGDGTTADDVYALGVLLLTLLAGRLPMANMDD
                     ATIIRWKLDLGSFAALTRDIPLSAS"
     gene            complement(905..2125)
                     /locus_tag="VGC82_10045"
     CDS             complement(905..2125)
                     /locus_tag="VGC82_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423651.1"
                     /translation="MVEKNHRLQRHLLIAGTGRAGTSFLVRYLDELGIDTILHRTGEN
                     AAWDEEANAGLEEMPFTSPDDDLPYVVKSPWLYQYIDELLADPAFIIDAVVIPVRDLA
                     EAVMSRCVIERRAMHQNAPWMGECSRIWEEWAQVPGGITYSLNPLDQGRLLAVGFHHL
                     VQRLVAADIPIIFLAFPRLVADATYLFNKLRPILPNTIDLEVACRAHRCTADPLKVRI
                     GTEIAAIDAVGKSRTTKIIGYDSQDPVDAVAVRREIGRLRKELSATHRAVAEAEAQMQ
                     SERRMAADREATLLQKIDAMEAMLAEAAATKERILLEDAETVRALHEKIYLGHQELYG
                     LNQQIAQLQQQALRDALRRREFETTLAAMRTSRSWRLTRPYRALGSILRRFVSATGIM
                     NVAGIHDRRDVHVV"
     gene            complement(2347..3549)
                     /locus_tag="VGC82_10050"
     CDS             complement(2347..3549)
                     /locus_tag="VGC82_10050"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003625238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0423652.1"
                     /translation="MPGFSQRLSRVSLAASVQMTIKARELKAAGKPVITLTTGEPNFE
                     SPPHAIEAAHQAALHGDTKYPPQDGTRALKQAIQRKFKRDSGLDYALDEIGVSNGGKQ
                     CLFNAIMATVDPGDEVIIPVPSWIAYAQMTELCEGVPVLVSCPQNHGFKPRAEDIDAA
                     VTPRTKWLILNNPNNPTGACCSAAEIAEIAAVMRKHPHVWILSDDMYEHLVFDGFQHA
                     TFAQVAPDLKDRTLTVSGVSKTYAMTGWRVGFAGGPKPLIRAMTVMQGQATAGISTVG
                     QAAAAAALDGPQDGVDVQIEAYKRRRDLAVDMLNAAKGISCHKPEGAFYVFPNVAGCL
                     GKTTTGGRKLDTDEDVCLALLEESYVATVHGAAYHMSPFLRISTATADDELLEGLRRI
                     QTFCEGLR"
     gene            complement(3551..4753)
                     /locus_tag="VGC82_10055"
     CDS             complement(3551..4753)
                     /locus_tag="VGC82_10055"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0423653.1"
                     /translation="MSLTSERLDRIQPSLTIAISTVARRMIAEGKNVISLSQGEPDFD
                     TPRNIKDAAIRALENGETKYTDVSGTQALRKAVAEKFRRDRGIVYKPEEIIISTGAKQ
                     VLFNAMVGTLNAGDEVIIPSPCWVSYPDIVTLADGSPVLVPCGQNNGFKLRAEDLEAA
                     ITPKTRWFMLNNPCNPTGAAYDAEDLKPICEVLMRHPHVWVLTDDIYDSLVYGGFKPA
                     TILQVEPRLRDRTLTVHGVSKAYAMTGWRIGFAGGPVALIKAMDKLQSQSTSNPNSIA
                     QAAAVEALLGQQDTVEEMRQVFEQRRDLVVEMLNRAPGMNCSKPDGAFYVYPSIHGCI
                     GKTSKGGVLIKDDETFALTLLSEEGVATVHGAAFCYPGYIRISYANATDELREACTRI
                     QRFCQGLH"
     gene            4956..5282
                     /locus_tag="VGC82_10060"
     CDS             4956..5282
                     /locus_tag="VGC82_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEY0423654.1"
                     /translation="MIYELRIYECVPGRLPDLNKRFSTITLKIWERHGIKQAGFWTTL
                     IGESNMTLYYMLAWESLADRETKWNAFQADPEWHAARAKTEENGPIVAKVTNYILGPT
                     PYSSVK"
     gene            5509..6444
                     /locus_tag="VGC82_10065"
     CDS             5509..6444
                     /locus_tag="VGC82_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423655.1"
                     /translation="MAEEWDWNVAVFCRNEQASIARCIESIAQASRTKRAVITLIVNG
                     CTDNSTATALAAASRCGVAITIYEIAHGDKANAMNRFFHDLRESAEYYFFVDAYARIG
                     LKALLAMETCLATRPDVVAATGVAVNGRTMHRQTEATLMQGGQLHGQLHALRKDFIDR
                     LVARKIRIPIGLYYGDGLVGSMAMHDLDSRHNPWEPKRIGGSADATYEIPVLSPYRLQ
                     DVKRQFHRKTRQMRGRLENLAIRAIIYDHGYEGLPESADDMINAYLAQHQLPRPSLLG
                     RVFQAQALRQIRASRKPDPVRLRPVLVSRLKRTVI"
     gene            complement(6441..>7277)
                     /locus_tag="VGC82_10070"
     CDS             complement(6441..>7277)
                     /locus_tag="VGC82_10070"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HEY0423656.1"
                     /translation="TRRRNQMMNDMPTTQDAVPLDSKAVADLGEQLNQLLRLRTFPIG
                     MKLFEDIAEMEAVPGLRRPAKGKTYSTCQLVTQSRMAGFTLGITTENVPPFSSCSSVI
                     GLDAPGEIYTSGRKMEGVWFENREASAAHQAQMPRVTPGTYHGMAISPLRTGRLSPPD
                     VCLFYGNPAQMILFINGLQWRNYQRYDFSITGESACADSWGSALKNKKVSLSIPCYAE
                     RRYGGVADDEMLMACPPADLLRAVTGLQGLAKSGLRYPIMPFGPQAEPAEGMAKSYAG
                     KS"
BASE COUNT         1388 a         2222 c         2259 g         1408 t
ORIGIN      
        1 aaggacgcag agagcggaat gtcacgagtc agtgcggcga agcttcccag atcgagcttc
       61 cagcgaataa tcgtcgcgtc gtccatgttc gccatcggaa gccggcccgc cagcaacgtg
      121 agcaacagaa cgcccagcgc atagacgtcg tcggcggtcg tgccgtctcc ccttccggtc
      181 ggatggcaca tcgcgctgta cgggcattcg aacacgacgg gctgatgcat cgcgggtggc
      241 gccgcccatg ccgcaccaag gacgaccggc tgacccggag ctgactggaa gacattgttc
      301 gggcggattg cacggtgcgt cagcttgcgg ctgtgcagga cttccagcac ccgcgccaca
      361 ggtcggagca cgagatcgat cagcgccttt tccggccaag cgttcagcgg cgccgatacc
      421 gagggaccgg gcggtggcgt gcagacgata aagtagcctt cgccttttcc tccggccagc
      481 ggcgcgatgc catgaccgag cggccccatc aggttgtcga tggggtcttt taacgctttc
      541 agggcattgg cgcggggcga ggcatcccga gaaacggcga tggcgatgcg cttgccatcc
      601 gaagccaacc ggtcgcgcgc gagatacgcc tttagccctc cgccggcatc gggcaggatc
      661 tgcgaactgt cgacggcaaa acggccggcg atcagcgtgt cggaaagtgg aacaagcggg
      721 tcatcagcca tgcgggcgaa ccattttggt cgggccgcgg catcttatcg gtgaaaaggt
      781 tgactgaagg ttaacagcga aaggatcttg taccatcagg tgcgatcata ccgagccagc
      841 ccggcacgac gtgtcggtct caacgccgtc gcccgaccat cgccgacagg aagaatatgt
      901 tcgatcagac tacgtggaca tcgcgccggt catgaatgcc agccacgttc atgatgccag
      961 tagccgatac aaaacgccgc aggattgatc cgagggcgcg atatgggcgg gttagccgcc
     1021 acgatctgga ggtgcgcatt gccgcaaggg tggtttcgaa ctcccgccgg cgtaaggcat
     1081 cccgaagcgc ctgttgctga agttgagcta tttgctgatt cagcccgtac aactcttgat
     1141 ggcccaaata gatcttttca tggagcgccc gcacggtctc cgcgtcctcc agtaaaatcc
     1201 gctcctttgt tgccgccgct tcagcaagca tggcctccat cgcgtcgatc ttttgaagca
     1261 gggtggcttc gcgatcggct gccatgcggc gttccgactg catttgtgct tcggcctcag
     1321 cgacggcacg atgtgtggcg gagagttcct tacgaagccg tccgatttct cgccgcacgg
     1381 caactgcgtc aaccggatcc tgcgaatcgt aaccgatgat tttcgtggtg cgcgactttc
     1441 caaccgcatc aatcgccgcg atttctgttc cgatcctgac cttcaatggg tcagccgtgc
     1501 accgatgtgc tcggcatgcc acttcgaggt caattgtatt cggcaaaatc gggcggagtt
     1561 tgttgaacag ataagttgcg tccgcgacta accgcgggaa agccaggaaa atgattggga
     1621 tatcggccgc aaccagacgc tgaacaagat gatgaaagcc gacggccagg agacgtcctt
     1681 ggtcaagggg attgagggag taggtgatcc cgccgggaac ctgagcccac tcctcccata
     1741 ttcgactaca ttcgcccatc cagggagcgt tctggtgcat tgcgcgtcgc tcgataacgc
     1801 agcggctcat gacagcctcg gccaaatctc gtacgggaat gacaacagcg tcgattatga
     1861 aggcggggtc agccaaaagt tcgtcgatat attggtacaa ccacggcgat ttaacgacat
     1921 aaggcagatc gtcgtccggg ctggtgaatg gcatctcctc cagaccggcg ttcgcttcct
     1981 catcccaggc agcgttttcg cctgttcggt gaagaatagt atcgatccca agctcatcaa
     2041 gataacgaac cagaaagctt gtgccggcac ggccagttcc ggcaatcaaa agatgccgct
     2101 gtaaacgatg gtttttctcg accattcagt atgctttcgg ctgatacgag gggtatcatt
     2161 ctctccgtat gtttcccgaa tgtgtcagga acagactcaa gcagatatca tgcccgagcg
     2221 atcagacgaa gatcccatgc tggccccaat aacgcgggcg aacgatttgc atgagctcgt
     2281 gtcggacttg cgaacgccgc gccttggccg ggaccggcca caccagccca tgtctcctgc
     2341 agaccttcag cgcaagcctt cgcagaaggt ctggatgcgc ctcaggcctt caagcaactc
     2401 atcgtccgcg gtggcggtgc tgatgcgaag gaacgggctc atgtgatagg cggcgccatg
     2461 aacggtggcg acgtagcttt cttccagcag ggccaggcag acatcctcgt cggtatccag
     2521 cttccggcca ccggtcgtgg tcttgcccag gcagccggcg acgtttggga acacgtagaa
     2581 agcgccctcg ggcttgtggc aactgatgcc tttcgccgcg ttcagcatat cgacggccag
     2641 gtcgcggcgg cgcttatagg cctcgatctg cacatccacg ccatcctgcg ggccgtccag
     2701 cgccgccgcc gcggcggcct gaccgacggt ggaaatgccg gccgttgcct ggccttgcat
     2761 gaccgtcatc gctcggatca gcggcttcgg accaccggcg aaaccgacgc gccaccccgt
     2821 catggcatag gtcttcgata cgcctgatac cgtcagcgtc cggtccttca ggtccggcgc
     2881 gacctgagca aacgtcgcgt gctggaagcc gtcgaacacc agatgctcgt acatgtcatc
     2941 ggacaggatc cagacatgcg gatgcttgcg catcaccgcg gcaatttcgg caatctcggc
     3001 ggcggagcag caggcgccgg tcgggttgtt ggggttgttc aggatcagcc acttggtgcg
     3061 cggcgtgacc gcggcgtcga tgtcctcggc gcgcggcttg aagccatgat tctgcggaca
     3121 gcttaccagc accggaacgc cctcgcacag ttcagtcatc tgtgcgtagg cgatccagga
     3181 tgggaccgga atgatgacct cgtcgccggg atcgaccgta gccatgatgg cgttgaacag
     3241 gcactgcttg ccgccgttgg aaacgccgat ctcatccagc gcgtaatcca ggccggagtc
     3301 gcgcttgaac ttccgctgga tcgcctgttt cagggctcgg gtgccatcct gcggcgggta
     3361 cttggtgtcc ccgtgcaacg ccgcctggtg cgccgcctcg atcgcgtgcg gcggactttc
     3421 gaaattgggt tccccggtcg tcagggtgat caccggcttg cctgcggcct tcagttcgcg
     3481 cgccttgatc gtcatctgga cgctggcggc cagcgagacg cggcttaaac gttgcgagaa
     3541 accgggcatg tcaatgcagg ccttggcaga agcgttgaat tcgggtgcag gcttcgcgca
     3601 gttcgtcggt cgcgttggcg tagctgatcc ggatgtaacc ggggtagcag aaggccgcgc
     3661 cgtgaacggt cgctacccct tcctcggaca gcaaggtcag ggcgaacgtc tcgtcgtcct
     3721 tgatcaggac gccgcctttg ctggttttgc caatgcagcc gtggatcgag ggatagacgt
     3781 agaacgcgcc gtccggcttg ctgcaattca tgccgggcgc ccggttcagc atctcgacca
     3841 ccaggtcgcg acgctgttcg aacacctgtc gcatttcctc cacagtatcc tgttgcccga
     3901 gcagggcctc gacggcagcg gcctgcgcga tcgagttggg gttggaggtg gactggcttt
     3961 gcagcttgtc catcgccttg atcagcgcca cgggaccgcc ggcgaagccg atacgccagc
     4021 cggtcatcgc ataggccttc gacacgccgt gcacggtcag cgtgcggtcg cgcagcctcg
     4081 gctcgacctg caggatcgtc gctggcttga agccgccata gaccaggctg tcgtagatgt
     4141 cgtcggtcag cacccagaca tgcggatggc gcatcagcac ctcgcagatc ggcttcaggt
     4201 cttccgcgtc ataggccgcg ccggtcgggt tgcaggggtt attgagcatg aaccagcggg
     4261 ttttgggcgt gatcgccgct tccagatcct cggcgcgcag cttgaaaccg ttgttctggc
     4321 cacaggggac caggaccggg ctgccatcgg ccaaggtgac gatatcgggg taactgaccc
     4381 agcaggggct ggggatgatg acctcgtcgc cagcgttcag ggtgccgacc atggcgttga
     4441 acagcacctg cttggcaccc gtggagatga tgatctcctc gggtttgtaa acgatgccgc
     4501 ggtcgcgccg gaatttctcg gccacggcct tccgcagtgc ttgggtcccg gacacgtcgg
     4561 tgtatttcgt ctcgccattt tcgagcgccc ggatggcggc gtccttgatg ttgcgaggcg
     4621 tatcgaagtc gggctcgccc tgcgacaggc tgatcacgtt tttcccttcg gcgatcatgc
     4681 ggcgcgccac ggtggaaatc gcgatggtca ggctgggctg aatgcgatcg agacgttcgg
     4741 acgtaaggga catggaaggt tccggggagt ttgtcggagg gataagccag gcacgcggaa
     4801 gcgcgggacc ctatccaagc ggtgccacgg gggcaaccgt tcgctttgca gcgcaggtag
     4861 gtgccaacgt gggtccgcgc cgcaaggacc ggcccgcttg ccggccgaag ggggtggctg
     4921 caaactgcac ggggtgcaac ccctgggagg gaaccatgat ctacgaattg cgaatctatg
     4981 aatgcgtccc cggccgtctg ccggacctga acaagcgctt cagcaccatc acgctgaaaa
     5041 tctgggaacg ccatggcatc aagcaggccg gtttctggac gacgctgatc ggcgagtcga
     5101 acatgacgct gtactacatg ctggcttggg aatcgctggc cgaccgcgag accaagtgga
     5161 acgccttcca ggccgatcct gaatggcatg ccgcccgcgc caagacggag gaaaacgggc
     5221 cgatcgtcgc caaggtgaca aactacattc tcggtccgac cccttattct tcggtcaagt
     5281 agcgcctcac tcttcggtca agtagtgcct cggtcgccgt gtcatgcagt tacggcgcgg
     5341 cgaccggtga ctttgcagca cccggggcgt ttctgtggtg taccaaccgt ttcctcggct
     5401 aatcggtgcc ggattgggcg aaccggaaaa accgatccga acgaatccat aattatgatc
     5461 gaacctgtgc catacaggcc aggtcaggct cgtggaggcg aagttgtagt ggcggaggaa
     5521 tgggactgga acgtcgccgt gttctgccgc aacgagcagg cgtcgatcgc gcggtgcatt
     5581 gaaagcatcg cccaggcgag ccgcaccaag cgcgccgtca tcaccctgat cgtaaacggc
     5641 tgcaccgaca acagcacggc tacggcactt gcagctgcaa gccgctgcgg cgtcgcgatc
     5701 accatctatg aaatcgcgca tggcgacaag gcgaacgcca tgaaccgctt cttccatgac
     5761 cttcgggaga gtgccgaata ctatttcttc gtcgatgcct acgcccggat cggcctcaag
     5821 gcgcttctgg cgatggaaac ctgcctggcg acccgtcccg atgtggtcgc ggccaccgga
     5881 gtcgcggtga atggccggac catgcatcgt caaaccgagg cgacactgat gcagggcggc
     5941 cagcttcatg gccagttgca cgcgctgaga aaggacttca tcgaccgcct cgtagcgcgc
     6001 aaaatccgga tcccaatcgg gctgtactac ggcgacggac tggtcgggtc gatggccatg
     6061 cacgacctcg actcgcggca taacccgtgg gagccgaaac gcatcggcgg cagtgcagac
     6121 gccacctacg aaattccggt gctgtcgccg taccgccttc aggacgtgaa gcggcagttc
     6181 catcgcaaga cccgccagat gcgaggccgg ctggagaacc ttgcaatccg cgccatcatc
     6241 tatgatcacg gctacgaagg gcttcctgaa tccgccgacg acatgatcaa tgcgtatctc
     6301 gcgcagcatc agctgcctcg gccatcgctg ctcggtcgcg tatttcaggc gcaggcgctg
     6361 cgccagatac gcgcatcccg aaagcctgat ccggttcggc tgcggccggt tctggtcagc
     6421 cgccttaagc ggaccgtaat ctagcttttg ccagcgtaac ttttcgccat accctctgcc
     6481 ggctcggcct gaggtccgaa cggcatgatt ggatagcgca ggccggattt cgccagaccc
     6541 tgcaatccgg tcaccgcgcg cagcaggtcg gcgggcgggc acgccatcag catctcatcg
     6601 tccgccacgc cgccatatcg ccgctccgcg tagcagggga tcgacaggct gaccttcttg
     6661 ttcttcaacg cgctgcccca gctatcggcg caagcgctct cgccggtgat actgaagtca
     6721 taacgctgat agttgcgcca ctgcagaccg ttgatgaaca ggatcatctg cgccggattg
     6781 ccatagaaca ggcacacatc gggcgggctg agccgccccg tccgcagcgg cgaaatcgcc
     6841 attccgtggt aagtgccggg ggtgacccga ggcatctgcg cctgatgcgc cgccgaggct
     6901 tcgcggttct cgaaccacac accctccatc ttacggccgg aggtgtagat ctcacccggc
     6961 gcatccagcc cgatcacact gctgcagctc gagaacggcg gcacgttctc cgttgtgatg
     7021 ccaagggtga acccggccat ccgcgactgg gtcacaagct ggcaggtgga gtaggtcttt
     7081 cccttggccg gccggcgaag accgggaacg gcctccattt cggcaatgtc ctcgaacagc
     7141 ttcatgccga tcgggaaggt gcgaagccgg agcagttgat tgagctgttc gcccaggtcg
     7201 gcgaccgctt tgctgtccaa gggaacggcg tcctgcgtcg tgggcatatc gttcatcatc
     7261 tggttcctcc tgcgagt
//
LOCUS       DASWQN010000597         8003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_586011, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000597 DASWQN010000000
VERSION     DASWQN010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586011"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VGC82_10075"
     CDS             <1..504
                     /locus_tag="VGC82_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423657.1"
                     /translation="FGMMFAAESVLFALSVTILATELTEPVAGAERDDLGRLLQSMLI
                     LWAYLDFMQLLIVWQSNLPKEAAWYVARTAGPWGIIAGVTAVGHFLLPFLILLAPALR
                     RSRRGIIATTTLLIAMSIIRGWWLVLPAQGRGIGWIDIAAIVALGGLSVGMTTRGLGP
                     RLRVTHA"
     gene            497..850
                     /locus_tag="VGC82_10080"
     CDS             497..850
                     /locus_tag="VGC82_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423658.1"
                     /translation="MPEPVLPPAGYEHSDVTTRSALVAFPAILVGLLLVVLVVRWIYP
                     GTAIDRRLQSPLPVFPSPRLQSSPSADMQTFLKQELARLNSGGWDDEAKGAGHIPIQE
                     AMRRVAAAGIADWPK"
     gene            847..1602
                     /locus_tag="VGC82_10085"
     CDS             847..1602
                     /locus_tag="VGC82_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0423659.1"
                     /translation="MIVRTLLLIVLLGSDAWAAGDLSGFAYDQRLGEPLPLTATFTDE
                     YGGEVTLRQLLRQRPAILALSYFHCPNLCGLVRDDLLNALSRMKSGQDYGVVVLSIDP
                     SETPADAKAAGATDMARYGTTADLHYVTGSASAIRAVTDAVGFRSRFDPETKQFLHPA
                     GIVFLTGDGTVSGYLLGLGYQPGDVSLGITRAAGGIGARALPVLLLCFHFDPVTGRYT
                     LAVMRVLQLGAAITVLVVGGTMMLALRRERRPR"
     gene            1599..2528
                     /gene="coxB"
                     /locus_tag="VGC82_10090"
     CDS             1599..2528
                     /gene="coxB"
                     /locus_tag="VGC82_10090"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY0423660.1"
                     /translation="MKFTLPLAASTGAETDFLLLGLVAVSIAVLALVFGLMFLYVVRY
                     RHNSPSQRGAIAEKSFRFEATWTAATLLIFFGLFIWGSVIYVRLYQPLADALKIYVVA
                     KQWMWKAEHAGGQREINALHLPIDTPIELVMTSQDVIHDFFVPAFRIKRDVLPGQYQT
                     MWFRADRVGTYHLFCSQFCGTLHSGMIGDVVVMSSGDYADWLTTNGAGDTLADQGKAL
                     FVRYGCAGCHFGNGTVRAPSLARLYGSPVPMSDGSTIIADDRYIRDSIMYPKQQIVAG
                     YEPVMPSFANVINEDDLVRLVAYIHSLAAEKRG"
     gene            2525..4144
                     /locus_tag="VGC82_10095"
     CDS             2525..4144
                     /locus_tag="VGC82_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0423661.1"
                     /translation="MSQIVLPQSQISYLTDGVSLKSWLLTTDHKRIGILYMISITVFF
                     FLGGSAAALIRYNLLLPEGAIESAETYNRLFTMHGVIMVWFFLVPAVPVTIGNFVVPL
                     MLGARDLAFPKLNLISWYLFVAGGSCALYALCAGGVDTGWTFYTPLSSNYAQGHVVAV
                     VIGIFISGFSTIATGLNFIITIHRLRAPGLTWYRLPVFIWSLYSTSIIFVLATPVLAT
                     TLLLLLFERVFHVGVFDPRLGGDPLLFQHMFWFYSHPAVYIMILPGLGIVSEIIPVFS
                     RKQLFGYKFVVWCSVAIAVIAFFVWGHHMFVSGQSLFASVVFSLLSYFVAVPSAIKVF
                     NWLGTLHKGHIRFDAPMIYALGFIGLFTLGGVTGLFLAALAADVHLHDTYFVVAHFHY
                     IMVGGMVSAYFAGLHYWWPKITGKMYPEMIARAAALLMFLGFNLTFFPQYILGYLGMP
                     RRYHAYPPEFQVLNVLSSAGATILAAAYLMPLLYLTFSIFRGERAPANPWGGLGLEWQ
                     TPSPPFTKNFTVTPTVTKGPYDFPEAGAVDV"
     gene            4137..4784
                     /locus_tag="VGC82_10100"
     CDS             4137..4784
                     /locus_tag="VGC82_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY0423662.1"
                     /translation="MSEEVAPHTHFQFADAAHQADTALCGMWLFLASEILFFGGLIIA
                     WMFCRHWQLAGFDAGARETVLWIGTLNLVLLITSSFVYSTGLAFTEAGNIRRLVQCCG
                     VTMAIGTLFLLLKFYEWHVDFSENLFPYGDFKITGADAGGARMFWSFYFVATALHAVH
                     MIVGIGLVGWVAMQARRGEFDDGWFTPAEVVGLYWSFVDIVWIVLYPIIYLVGRG"
     gene            4781..5068
                     /locus_tag="VGC82_10105"
     CDS             4781..5068
                     /locus_tag="VGC82_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423663.1"
                     /translation="MKQRHRLDLLVWLALLIVAAMEFGASFLPIAAGTRPILMLPAAI
                     MAVLVALGYMRLLTAPEIARAFAVAGIFWLTVLLGLAMTDPLTRTVYAVAG"
     gene            complement(5081..6241)
                     /locus_tag="VGC82_10110"
     CDS             complement(5081..6241)
                     /locus_tag="VGC82_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006449176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY0423664.1"
                     /translation="MRIVDVREVTKPISSPIRNACIDFSKMTSSLVAVVTNVQRNGKT
                     VVGYGFNSNGRYGQGGLIRERFAPRILEADPTSMLDGTGDNLDPDKVWVTMMRNEKPG
                     GHGDRSVAVGTIDMAVWDAVAKIAGKPLFRLLAERHGIVADPNIFVYAAGGYYYPGKD
                     DSALCAEMRSYLERGYGVVKMKIGGESIADDRRRIEAVLREIGHARLAVDANGRFDLE
                     TAIGYAKMLRDYDLFWFEEAGDPLDYQLQAALAEFYPGPMATGENLFSHQDARNLIRY
                     GGLRPDRDWLQFDCALSYGLCEYQRTLAMLKDHGWSWKRCVPHGGHQMSLNIAAGLGL
                     GGNESYPDVFQPYGGFPDGTQVRDSIVTLPDLPGIGFEAKSDLYSIMRDLAA"
     gene            complement(6418..6870)
                     /gene="hemJ"
                     /locus_tag="VGC82_10115"
     CDS             complement(6418..6870)
                     /gene="hemJ"
                     /locus_tag="VGC82_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HEY0423665.1"
                     /translation="MTIPALSNLYPWTKAFHVISLIAWMAAIFYMPRLFVYHCDLRSG
                     SAESERFKVMEYRLFKQIMNPAMIATWVFGITLVLTPGTVDWSSGWWHVKLFCVILMS
                     GFHGAMSRWRKDFMADANRRSQKFYRIANEIPTVLMVVIVIMIIVRPF"
     gene            complement(6867..7559)
                     /locus_tag="VGC82_10120"
     CDS             complement(6867..7559)
                     /locus_tag="VGC82_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY0423666.1"
                     /translation="MNCKRTNLGLVIFDCDGVLIDSEALCNRVVSEELTRQGWAITPT
                     DCHRRFVGLTFADTKEVAEAHLGRSLGPGWIDTITQRVTEVMAEEANLIAGARQALEA
                     TIAMGLPFRVASNSSRQEMAAKFARTGLASLIGNRIHSAHDLIAAGKRGKPAPDLFLE
                     AAKAENVRPEACLVIEDSLAGVQAAMAAGMNCLGFSPEDDGAHLRLAGALPFASMFAL
                     PDLLRGFLHRTL"
     gene            complement(7760..>8003)
                     /locus_tag="VGC82_10125"
     CDS             complement(7760..>8003)
                     /locus_tag="VGC82_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423667.1"
                     /translation="ILKEALEKCGAADRRKVSAAIRDLDMTDGPALFFPDGRLKYDEK
                     GRRVGAKLCVVQWRAGKPVPVYPPEIATHDVIWPKA"
BASE COUNT         1345 a         2445 c         2459 g         1754 t
ORIGIN      
        1 ttcggcatga tgttcgcagc ggaaagcgta ctgttcgcac tgtcggtcac catcctggcc
       61 accgaactga cggaacccgt tgctggagcc gagcgggatg acctggggcg gcttttgcag
      121 agcatgctga tcctgtgggc atatctcgat ttcatgcagt tgctgatcgt ttggcaatcc
      181 aatttgccga aagaagctgc gtggtatgtg gcccgaacgg cgggcccgtg gggcattatc
      241 gcaggggtga ccgcggtcgg tcacttcctg cttcccttcc tgatcttgct cgctccggcg
      301 ctccggcggt cgcggcgcgg catcattgcc acgaccaccc tgctgatcgc catgtccatc
      361 atccggggct ggtggctcgt gcttcccgca cagggccgag ggatcggctg gatcgacatt
      421 gctgcgatcg tcgcgctggg cgggctttcg gtcggcatga cgacgcgggg cctcggtccg
      481 cggttgcggg tgacccatgc ctgagcccgt gctccccccc gccggttatg agcactcgga
      541 cgtcaccacg aggtccgcgc ttgttgcgtt cccggccatc ctggtcggct tgctgctggt
      601 ggtgttggtc gtaagatgga tctatcctgg caccgccatt gaccgccggc tgcaatcgcc
      661 gctgccggtg ttcccctcgc cgcggctgca atccagccca agcgccgaca tgcagacatt
      721 cctgaaacag gagcttgcgc gtctgaacag cggtggctgg gatgacgaag caaaaggcgc
      781 cggccacatt cccatccagg aggcgatgcg ccgggttgcc gccgccggca tcgcggattg
      841 gccgaaatga tcgttcggac attgctgctg attgttctcc ttggctcgga tgcctgggcc
      901 gccggcgacc tgtccggctt tgcctatgac caaaggttgg gcgaaccatt gccgctgacc
      961 gcgaccttca ccgatgagta cggcggcgaa gtcacgttgc ggcaactgct ccggcaacgt
     1021 ccggctattt tggcgctgag ctactttcac tgtcccaact tgtgcggact ggttcgcgac
     1081 gatctgttga acgcgctgtc ccgaatgaaa tccggtcagg attacggtgt tgtcgtgctg
     1141 tcgatcgacc cgtcggagac accggccgac gcaaaagccg ccggagccac cgatatggcg
     1201 cgctacggca caaccgcgga cctgcattat gtgaccggtt ccgcttcggc catacgggcc
     1261 gtcaccgacg ccgtgggctt ccggtcgcgg tttgacccgg agacaaaaca atttctccat
     1321 cccgccggca tcgtgtttct gacgggcgat ggcaccgtgt cgggctacct gctggggctg
     1381 ggctatcagc cgggcgacgt cagcctgggg atcacccgag cagcgggcgg catcggcgcg
     1441 cgggcgctgc cagtcttgct gttatgcttc cattttgatc cggttaccgg gcgttacacg
     1501 ctggcggtca tgcgagtgct gcaactgggt gccgcgatca cggtcctcgt ggtcggcggg
     1561 acaatgatgc tcgcgttgcg gcgggaaagg cggccgcggt gaagtttacg cttccgctcg
     1621 ccgccagtac cggtgccgag accgatttcc ttctgctcgg tcttgtcgcc gtctccattg
     1681 ccgtgctggc gttggtattt ggcctgatgt ttctctacgt cgtccgctat cgccacaaca
     1741 gcccgagcca gcgcggcgcc atagccgaaa aatcgtttcg cttcgaagcg acatggaccg
     1801 ccgccacatt gttgatcttc ttcggcctgt tcatctgggg gtcggtcatt tacgtccggc
     1861 tttaccagcc tctggcggac gcgctgaaga tctacgtcgt cgccaagcaa tggatgtgga
     1921 aagccgaaca tgccggtggc cagcgcgaga tcaacgccct gcacctgccg attgatacgc
     1981 cgatcgagtt ggtcatgaca tcccaggacg tgatccacga cttctttgtt cccgccttcc
     2041 ggatcaagcg cgacgtgctg cccggccagt accagacgat gtggttccgg gcggatcgcg
     2101 tcggcaccta tcacctgttc tgttcccagt tttgcggcac actccactcc ggcatgatcg
     2161 gtgacgtggt ggtgatgtct agcggagatt atgccgactg gctgaccacc aatggtgcgg
     2221 gcgatacgtt ggccgaccag ggcaaggcac tgttcgtgcg ctacggatgc gcgggttgtc
     2281 acttcggcaa cggtaccgtt cgagcaccgt cgttagcgag gctgtacggc agtccggtgc
     2341 cgatgtccga cggcagcacg atcatcgccg acgaccgcta tatccgcgat tccatcatgt
     2401 atccgaagca gcagatcgtt gccggctacg agccggtgat gccgtcgttc gccaacgtca
     2461 tcaacgagga cgacctggtc cgtctggtcg cttatatcca ttcactcgcc gcggagaagc
     2521 gcggatgagc cagatcgtgc ttccgcagtc ccagatcagc tacttgaccg acggcgtgtc
     2581 gttgaaaagc tggttgctga cgaccgacca caagcggatc gggatcttgt acatgatctc
     2641 gatcaccgtt ttctttttcc ttggcgggtc cgcggcggcg ctgatccgct acaacctgct
     2701 gctgcccgag ggcgccattg aatcggccga gacctataac cgtctgttca cgatgcatgg
     2761 cgtcatcatg gtgtggtttt tcctggtgcc cgccgtcccg gtgacgatcg gcaacttcgt
     2821 cgtcccgttg atgctgggcg ccagggacct cgcgttcccg aagctcaatc tgatcagttg
     2881 gtacctgttc gtcgccggag ggtcctgcgc gctttacgcg ctgtgcgccg gcggcgtgga
     2941 caccggctgg accttctata cgccgctatc cagcaactac gcccagggcc atgtggtggc
     3001 ggtggtgatc ggcatcttca tctcgggctt ctccaccatt gcgaccgggc tgaacttcat
     3061 cattaccatc caccgtctgc gtgcgccggg actgacctgg tatcgcctgc cggtgtttat
     3121 ctggtcgttg tattccacca gcatcatttt tgtgctggcg acgcctgtac tggcgacgac
     3181 attgctgctg ctgttgttcg agcgggtttt ccacgttggt gtcttcgatc cccgcctagg
     3241 cggggatccg ttgctgttcc agcatatgtt ctggttctac tcgcacccgg cggtttacat
     3301 catgattctg cctggccttg ggatcgtctc ggagatcatt ccggtatttt cccgaaagca
     3361 actattcggc tacaaattcg tggtctggtg cagcgtcgcc atcgcggtga tcgcgttctt
     3421 cgtgtggggt caccacatgt tcgtgtccgg ccagtcgcta ttcgccagcg tggttttctc
     3481 gttgctgagt tacttcgtcg cggtgccttc cgctataaag gtgttcaact ggcttggcac
     3541 tttgcacaag ggccacatcc ggttcgatgc tccgatgatc tatgccctgg gcttcatcgg
     3601 tttgttcacg ctgggcgggg ttaccggcct gtttctggcc gcactggcgg ccgatgtcca
     3661 tctgcacgac acctatttcg tcgtcgcgca tttccactat atcatggtgg gcgggatggt
     3721 atcggcttac ttcgccgggc tgcattactg gtggccgaag atcaccggga aaatgtatcc
     3781 cgagatgatc gcccgcgccg ccgcgttatt gatgttcctg ggcttcaacc tgacattctt
     3841 tccgcaatac atcctgggct acttaggcat gccccgccgt taccatgcct atccgccgga
     3901 gttccaggtg ctgaacgtgc tgtcttcggc cggagctact attctggccg cggcctacct
     3961 gatgccgctg ttgtacctga ctttctccat cttccggggc gagcgtgccc ccgccaatcc
     4021 gtggggcgga ctgggtttgg aatggcaaac tccgtcgccg ccgttcacca agaattttac
     4081 cgtgactccg accgtcacta aagggcctta cgattttccc gaagcagggg cggtcgatgt
     4141 ctgaggaagt cgcgccgcat acgcattttc aattcgcgga tgcggcccat caggcggaca
     4201 cggcgctgtg cggcatgtgg ctgttcctgg cctctgaaat cctgttcttc ggaggcctga
     4261 ttatcgcgtg gatgttctgc cggcattggc aactcgcggg tttcgatgcc ggtgccaggg
     4321 aaacggtgct gtggatcggc acgctgaacc tggtcctgct gatcaccagt agtttcgttt
     4381 actccactgg acttgccttc accgaggccg gcaacattcg ccggctggtg cagtgctgcg
     4441 gcgtgaccat ggccatcggc acactgttcc tgctgctgaa gttctacgag tggcacgtcg
     4501 atttctcaga aaatctattt ccctatgggg atttcaagat caccggcgcc gacgcgggcg
     4561 gtgccaggat gttctggtcg ttttacttcg tcgccacggc acttcacgca gtgcacatga
     4621 tcgtcggaat cggcctggtc ggctgggtgg cgatgcaggc tcgtcgcggg gagttcgacg
     4681 atggctggtt caccccggcg gaggtcgttg gcctatactg gagttttgtc gatatcgtct
     4741 ggatcgtgct gtatccgatc atctacctgg tggggcgcgg atgaaacagc ggcatcgcct
     4801 ggatttgctc gtctggctgg ccttgctgat cgtggcggcg atggagttcg gcgcatcgtt
     4861 cctgccgatc gcggccggca cgcggccgat cctgatgctg ccggccgcga tcatggccgt
     4921 gctggtcgca ctgggctaca tgcggctgct cacagccccg gaaatcgccc gagccttcgc
     4981 ggtggcgggg attttctggc tgacggtgct gctggggctg gcgatgaccg atccgctgac
     5041 ccgcacggtc tatgcggttg ccgggtagga caccgcggct ctacgccgcc aggtcgcgca
     5101 tgatggaata aaggtccgac ttcgcctcga acccgatgcc cggcaggtcg ggcagcgtga
     5161 cgatactgtc gcgcacctgc gtgccgtcgg gaaagccgcc atagggctgg aatacgtcgg
     5221 gatagctctc gttgccacct agtcccaggc cggcggcgat gttcagcgac atctgatggc
     5281 cgccatgcgg cacgcaccgc ttccaggacc agccatggtc cttcagcatc gccagcgtgc
     5341 gctgatactc gcataggcca tacgacaacg cgcagtcgaa ttgcagccag tcgcgatccg
     5401 gacgcagacc gccatagcgg atcaggttgc gcgcatcctg gtgactgaaa aggttttcgc
     5461 ccgtcgccat cggccccggg taaaactccg caagcgccgc ctgaagctga tagtccaatg
     5521 gatcgcctgc ttcctcgaac cagaacaggt cgtagtcgcg cagcatcttc gcgtagccga
     5581 tcgccgtctc caggtcgaag cggccgttcg catcgacggc caaacgagca tggccgattt
     5641 cccgcagcac cgcctcgatg cggcggcggt catcggcgat tgactcgcca cctattttca
     5701 ttttcacgac gccgtatccg cgttccaggt agctgcgcat ctcggcgcac agggcgctgt
     5761 catccttgcc aggatagtaa tacccaccgg cggcgtacac gaagatgttg gggtcggcga
     5821 cgatgccatg ccgttcggcc agtaggcgga acagcggctt gccggcgatt ttcgccaccg
     5881 cgtcccagac cgccatgtcg atggtgccga cggcgacgga tcggtcgccg tggccgccgg
     5941 gcttttcatt gcgcatcatt gtcacccaga ccttgtccgg gtccagattg tcgccagtgc
     6001 cgtcgagcat cgaggtcggg tccgcctcca ggatgcgcgg tgcgaagcgt tcccggatca
     6061 agccgccctg gccgtaacgg ccgttggagt tgaacccgta tccaaccact gtcttgccgt
     6121 tgcgctggac gttggtgacc accgcgacga ggctggaggt catcttgctg aagtcgatac
     6181 aggcgttgcg gattggcgaa ctgatcggct tggtgacctc gcgaacgtca acgatgcgca
     6241 gtggcatgat aggcctccgt taagcaggat cgcgctgggc ggaacgtcca aggcgcgtga
     6301 atcatgcgat agacaaagcg cgcatgatcg cgctgggcgg aacgtccaag gcgggtgaat
     6361 catgcggtaa agcaagaaac agcatttccg gcctgtccca tcaggctgac gttcccttca
     6421 gaatgggcgg acgatgatca tgatgacgat aacgaccatc agcaccgtcg ggatttcatt
     6481 ggcgatgcgg taaaattttt ggctgcgccg gttggcgtcg gccatgaagt ccttgcgcca
     6541 gcgggacatc gcaccatgga agccggacat caggatgacg cagaacaact tcacatgcca
     6601 ccagccggac gaccagtcga cggtgccggg cgtcagcacc agcgtgatcc cgaagaccca
     6661 ggtggcgatc atggcggggt tcatgatctg cttgaacagc cggtattcca tgaccttgaa
     6721 gcgctcgctc tcggcactgc cggaccgtag gtcgcaatga taaacgaaca gccgcggcat
     6781 atagaatatc gccgccatcc acgcgatcag ggagatcaca tggaaggcct tcgtccaggg
     6841 atacagattg ctcagcgccg ggatggtcat agggtcctgt gcagaaagcc gcggagcaag
     6901 tctggtagcg cgaacatcga ggcaaaggga agggcgcctg caagacgcaa atgcgcgcca
     6961 tcgtcttccg ggctgaaacc gaggcagttc atcccagccg ccattgctgc ctgcacgccg
     7021 gccagcgagt cctcgatcac caggcaagct tcgggccgaa cattttctgc cttcgcggct
     7081 tcaagaaaca ggtcaggcgc gggtttgccg cgcttgccgg cggcaatcag gtcatgcgca
     7141 ctgtggatgc ggttgccgat gagtgatgct aacccggtgc gggcgaattt ggcggccatt
     7201 tcctggcgcg aggaatttga ggcaacgcga aacgggagcc ccattgcaat ggtagcttcc
     7261 aaggcttggc gtgccccggc aatgagattg gcttcctccg ccatgacttc ggtgacacgc
     7321 tgcgtgatgg tgtcgatcca accagggccc aggctgcgcc ccagatgcgc ctctgcgacc
     7381 tcctttgtat cggcaaaggt caggccgacg aagcggcggt ggcagtcggt gggcgtgatg
     7441 gcccagcctt gtcttgtcag ttcttccgac acgacacggt tgcacagagc ttcgctatcg
     7501 atcaggaccc cgtcgcagtc gaagataaca aggccgaggt ttgtccgctt acagttcaac
     7561 gaccgacgtc accgttcatg cgggaattcc tggtccatca ctctgatccg tccgtgtcgg
     7621 gcgtgggata tcgtttgacg tggatcatgt ccgcccgttg cttggcttgc cagagatcgt
     7681 aagccgcccg cacacctacc caggtttcgg ccttgcccca ggcggttgtt cacgatctta
     7741 ccaaagcggc gcggccgcct caggctttcg gccagatcac gtcgtgggtc gcgatctccg
     7801 gcggatagac cggaaccggc tttccagcgc gccactgcac cacgcacagc ttggcgccga
     7861 cgcgacgacc tttttcatcg tatttcagac gtccatccgg gaagaacaac gccgggccat
     7921 cggtcatgtc caggtcgcgt atcgcggcgc tgaccttgcg ccgatcggcg gcgccacact
     7981 tctccagcgc ttctttcagg atc
//
LOCUS       DASWQN010000598         5806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1113046, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000598 DASWQN010000000
VERSION     DASWQN010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113046"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VGC82_10130"
     CDS             complement(<1..606)
                     /locus_tag="VGC82_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HEY0423668.1"
                     /translation="MSGRPPASPIHRLRRYRPRPRRRPRYKGPSFNRMIPNIMTMLGL
                     CAGLTAMRFGLEGRFGAAAVAIAVAALIDGLDGRLARLLKATSRFGAEFDSLSDFVCF
                     GVAPPFVLYLWSLQRGGAYGYVPGLMFSVCMALRLARFNASLDGAPKPAYAYNFFTGV
                     PAPAGAGLAMFPLFLGLEANALGLDWLAAIAHHPLLCAPILV"
     gene            complement(603..1274)
                     /locus_tag="VGC82_10135"
     CDS             complement(603..1274)
                     /locus_tag="VGC82_10135"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEY0423669.1"
                     /translation="MALTDDIRSVLSPPHPAGRPFIMGGLIALLVGFAVGAWLVWTGL
                     MFTLFCLFFFRDPERVPPRRAGLFVAPADGRVVAVSPAAPPPELGLGSEPRWKVSIFL
                     TVLNVHVNRVPADGVVTRIAYRHGAFVSASLDKASASNERNALAIRLPNGQEIAVVQI
                     AGLIARRIVCSVREGDPVTAGDRFGMIRFGSRTDLYLPEGVVPLVIEGQTMIGGETVI
                     AELSS"
     gene            complement(1443..2822)
                     /locus_tag="VGC82_10140"
     CDS             complement(1443..2822)
                     /locus_tag="VGC82_10140"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003623971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY0423670.1"
                     /translation="MSNPGDADDTPLTSVQQMAEYLSAGCKPKDSFRIGTEHEKFGFR
                     KFDLTAPPYLPADGQPGSIRDLLAGLVRFGGAPILDGDNAIGVKQGAASISLEPAGQL
                     ELSGNTVATLHETKQELDDHFAQVRAACEGLDLGFVPLGFHPVATRAAMPWMPKGRYA
                     IMRRYMPLVGSMGLDMMTRTCTVQVNLDYASEADMRRKLRVSLLLQPLATALFANSPF
                     TEGKPNGFMSCRANVWTDTDNQRAGIPKVMFEDGFGFERYAAWLIENVPMYFVYRDGG
                     YIDVAGESFVAYMAGRVPALAGVTATVGDFADHVTTAFTDVRLKRFLEMRGADAGRAD
                     MMVAQSALWVGLLYDDAALAAAEALLRGAGWDDAVAARSAVPRKGLDAPWRGGTLRDI
                     VADVVTISQQGLRARGCRNAAGDDERMYLDPLEAIAAGAPTQAEHWLSRYHGAWKGDI
                     SRIFQEAAI"
     gene            complement(2927..3625)
                     /locus_tag="VGC82_10145"
     CDS             complement(2927..3625)
                     /locus_tag="VGC82_10145"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY0423671.1"
                     /translation="MIRLHVDAALAAGAVIEATEGQARYLGSVMRRTAGDTVHLFNGR
                     DGEWACRIASLSRGKATLAAETLVRPQAGDNDLWLMFALLKRDTTDLIVQKATELGVS
                     ALLPVFTERTNAGRVNLDRLRAIAADAAEQSERLTVPTVHPARQLHDGLADWPTARSL
                     FVALERANAPHLQPVCEPAALLIGPEGGFGPRDHAALDRCRFVRPVSLGPRILRAETA
                     AIVGLALLQVPRCA"
     gene            3656..4576
                     /gene="ubiA"
                     /locus_tag="VGC82_10150"
     CDS             3656..4576
                     /gene="ubiA"
                     /locus_tag="VGC82_10150"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEY0423672.1"
                     /translation="MERQPDLSFMHTDIVATGWVARFPRTWLPYLLLARVDRPIGTWL
                     LFLPGLWGILLAHASLPETVRLVTLFAVGSLVMRAAGCVVNDLWDRDIDRKVARTAGR
                     PLASGALRPRHALVFLMVLLLAGLVVLLQLNRLSQVLGVASLLLVGLYPLAKRVTWWP
                     QLMMGFTFGFGAPLGYAAASGRLDAAWGALYAAAILWDLGFDTIYAHQDREDDALVGV
                     HSTARLFGERTKPFLAACYAASVVVLALFGWLAGLGPWFYLMLVLPAGLLARQVIALN
                     IDDAAVCLRLFRANREVGLAIGLAILAGWL"
     gene            4684..5307
                     /locus_tag="VGC82_10155"
     CDS             4684..5307
                     /locus_tag="VGC82_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY0423673.1"
                     /translation="MRANTVLATARHVPEIRLHLATEITPIWQATEAWMAEHNVEPPF
                     WAFAWPGGQALARHILDNPSLVAGKRVLDFAAGGGVAAIACALAGAALVEASEIDPLA
                     LTAVGLNAAVNGAGVLQAGDVVGEACRWDLILCGDVCYEAPMTGHIMPWLRKMAGSAE
                     VWIADPNRSYLPKQGLLPLARYRIETTLELEDHLSREVTLYRVLAEG"
     gene            complement(5283..>5806)
                     /locus_tag="VGC82_10160"
     CDS             complement(5283..>5806)
                     /locus_tag="VGC82_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423674.1"
                     /translation="NAAVDLAIDGFSLTSLSADPASFVPHHLAVRSVLTGVPLGPLMA
                     LLQAAIAPNPDQAALQRQAAAVLSAPGSKAAIESVAFDAGPVRVKGSARFQPRADGDI
                     GVDLHLSASGIDAALAQMQGRPNLQGVVPFILMAKGIGRAQGDTVVWDISFGGGPLTI
                     NGMPFGQPSARTR"
BASE COUNT         1017 a         1882 c         1909 g          998 t
ORIGIN      
        1 caccaggatt ggcgcgcaca gcaagggatg atgggcgatc gcggccagcc aatccaggcc
       61 gagcgcgttc gcttccagcc ccaggaacag cgggaacatc gccagcccgg cacctgccgg
      121 tgccgggacg ccggtaaaga aattgtacgc gtaggccggt ttgggcgcac catccaacga
      181 tgcgttgaac cgcgccagac gcagcgccat gcagaccgag aacatcaggc cgggcacgta
      241 gccatacgcg ccgccgcgtt gcaacgacca gagataaaga acgaatggcg gcgccacgcc
      301 aaagcacacg aagtcggaca ggctgtcgaa ctcggcgccg aagcgcgagg tcgccttgag
      361 caaccgagcc aggcgtccgt ccagaccatc gatcagggct gcaacggcga tcgcgacagc
      421 cgccgccccg aaccgccctt ccagcccgaa gcgcatcgcc gtcaggccgg cgcacagccc
      481 cagcatggtc attatgtttg ggatcatgcg gttgaacgac gggcccttgt agcgcgggcg
      541 ccggcgtggc cggggtcgat aacgccgcag ccgatggatg ggcgatgccg gagggcggcc
      601 gctcatgacg acaattcggc tatcaccgtt tcaccgccga tcatcgtttg accctcgatc
      661 accaatggca caacgccctc gggcagatac aggtcggtcc ggctgccgaa gcgaatcatg
      721 ccgaaccggt cccccgccgt caccggatcg ccttcgcgca ccgaacaaac gatgcggcgc
      781 gcgatcaggc cggcgatttg cacaaccgcg atctcttgtc cgttcggcag gcggattgcc
      841 agggcgttcc gttcgttgga ggcgctggcc ttgtcgaggc tggcgctgac gaaggcgcca
      901 tggcggtagg cgatgcgggt tacgacgcca tccgccggca cgcggttgac atggacgttc
      961 agcaccgtga ggaaaatcga gaccttccag cgcggctcgc tgcccagccc gagttccggc
     1021 ggcggtgcgg cggggctgac tgccacgacg cggccatccg cgggcgccac gaacagcccg
     1081 gcccggcgcg gcggcacccg ttcgggatcg cgaaaaaaga acaaacagaa caaggtaaac
     1141 atcaggccgg tccagaccag ccacgcgccg acggcgaagc caaccaacag ggcgatcaag
     1201 ccacccatga tgaagggacg cccggcggga tgcgggggtg aaagaacgct gcgaatgtcg
     1261 tcggtcaggg ccattagtcg cgggcttatg aaggtttacc ttgcgccggg caaggcattg
     1321 tcgatcgatg atcgtggatg gcagcattga cggcagcggt ggctcgccgc actggcgcgg
     1381 cgagtgctca gacccatgca aaacaggtct tggcacgact gacgcttcgc cttgggctga
     1441 ttctaaatgg cggcttcctg aaagatccgg ctgatatcgc ccttccacgc accgtggtag
     1501 cgagacagcc agtgttccgc ctgggtcggc gcacccgccg cgatcgcttc caacgggtcc
     1561 agatacatac gttcgtcatc gccggcggcg ttgcggcagc ctcgggcacg aagtccttgc
     1621 tggctgatcg tcacgacgtc ggcgacgata tcgcgcagcg tgccgcctcg ccagggtgcg
     1681 tccaggccct tgcggggcac cgcggaccgg gcggcaaccg catcgtccca accggcgccg
     1741 cgtaacagtg cctcggccgc cgccagcgcc gcatcgtcgt aaagcagtcc gacccacagg
     1801 gcggattgcg ccaccatcat gtcggcgcgc cccgcatcgg cgccgcgcat ttccagaaag
     1861 cgcttcagcc ggacatcggt gaaagccgtc gtgacatgat cggcgaaatc gccaacggtg
     1921 gctgtcacgc cagccaacgc tgggacgcgg ccggccatgt aggcgacaaa cgattcgccg
     1981 gcgacatcga tataaccgcc gtctcgatag acgaaataca tcggcacgtt ttcgatcagc
     2041 catgccgcgt agcgttcaaa accgaagccg tcctcgaaca tcaccttggg aatgccggca
     2101 cgctggttgt cggtgtcggt ccagacattg gcgcgacacg acatgaagcc gttgggtttc
     2161 ccctcggtga acggagagtt ggcgaacaac gccgtcgcaa gcggctgcaa cagcagggaa
     2221 acgcgcagct tgcggcgcat atccgcttcc gaagcataat cgaggttgac ctgcacggtg
     2281 caggtgcgcg tcatcatatc caggcccatg ctgccgacca gcggcatgta gcgccgcatg
     2341 atggcatagc ggcctttggg catccacggc atcgccgcgc gcgtcgccac cgggtgaaat
     2401 cccagcggta cgaacccgag atcgagcccc tcgcacgccg cccgcacctg cgcgaaatgg
     2461 tcgtcaagct cctgcttggt ttcgtgcagg gtggccaccg tgttgccgga cagttcgagc
     2521 tgtccggccg gttccagcga tatcgaggca gcaccctgct tgacgccgat cgcattgtcg
     2581 ccgtccagga tcggcgcgcc gccgaatcga accaggcccg cgagcaggtc gcgaatgctg
     2641 cccggttgac catccgcggg aagatagggc ggtgcggtca ggtcgaattt gcggaagccg
     2701 aatttctcgt gttccgttcc aattcggaac gaatcctttg gtttgcagcc ggctgaaagg
     2761 tattccgcca tttgctggac cgacgtaagc ggtgtgtcgt cggcgtctcc gggattggac
     2821 atagggttcc caaggcattg tcggttgcta tcgccggatc ggcggccgga cgcaggtgcc
     2881 gaccgaagac ggtgggcaga cgcgcctcag cgtttaaacc atcgtactag gcacacctcg
     2941 gcacttgcaa caaggccagt ccgacgatcg ccgccgtctc ggcgcgcaaa atgcgcggac
     3001 cgagggagac cgggcggacg aatcggcagc gatccaacgc tgcgtgatcg cgcgggccga
     3061 agccgccctc cggcccgatc aacagggcgg cgggttcgca aaccggctgc agatgggggg
     3121 cgttcgcccg ctccagcgcc acgaacagcg atcgggcagt gggccagtcg gctaaaccgt
     3181 cgtgtagttg cctggccgga tgaaccgtcg ggacggtaag acgctcgctc tgttcggcgg
     3241 catcggcggc gatggcacgc aggcgatcca gattgacccg gccggcgttg gtccgttcgg
     3301 taaaaaccgg cagcagtgcg gaaaccccga gttcggtcgc cttttgcacg atcaggtcgg
     3361 tggtgtcgcg cttcagcagg gcgaacatca gccataaatc gttgtcgcca gcctgcggcc
     3421 gaaccagggt ttccgccgct aacgtcgctt tgccgcgcga cagcgacgcg atgcggcagg
     3481 cccattcgcc atcccgccca ttgaacaaat gaaccgtgtc gcccgcggtg cggcgcatca
     3541 cgctgccgag gtagcgcgcc tgcccttcgg ttgcctcgat cacggccccg gcggccaacg
     3601 cggcatcgac atgcaggcgg atcatcggat cagcatatga tgggttgacg gaaccatgga
     3661 acggcagcca gacctttcat tcatgcatac cgatatcgtc gccaccggct gggtcgctcg
     3721 gtttccccga acctggctgc cctacctgct gctggcgcgc gtggaccggc cgatcggcac
     3781 gtggctacta tttcttccgg gtctttgggg catcctgctc gcccatgcat cgcttccgga
     3841 gaccgttcga ctggtcacgt tgttcgccgt cggcagcctg gtgatgcggg ctgccgggtg
     3901 cgtggtcaat gatttgtggg atcgcgacat cgaccgcaag gtagcgcgta ccgccggacg
     3961 gccgcttgcc tccggagccc tgcgtccgcg tcatgccctg gtgttcctga tggtgctgct
     4021 gctcgcaggc ctggtggtgc tgctgcaact caatcgcctg tcgcaggtac tgggcgtcgc
     4081 ctcgttgctg ctggtcgggt tgtacccact ggccaagcga gtgacctggt ggccgcaact
     4141 gatgatgggg ttcactttcg gcttcggcgc cccgctggga tacgccgccg cttccggccg
     4201 gctcgacgcg gcctggggcg cgctttatgc cgcggcgatc ctctgggatc tggggttcga
     4261 cacgatctat gcccatcagg accgcgagga tgatgcgctg gtcggcgtcc attccaccgc
     4321 aaggcttttc ggcgagcgga caaaaccgtt cttggcagcc tgttacgcgg cgtcggtcgt
     4381 tgtgctggcg ttgttcgggt ggctggctgg cctgggaccc tggttctacc tgatgctggt
     4441 cttgccggcc ggcttgctgg cgcggcaggt cattgcgctg aacatcgacg acgcggcggt
     4501 gtgtctgcgc ctgttccggg ccaaccggga agtgggtttg gcgatcggtc tggcgatcct
     4561 ggcgggctgg ctttgaccca taccgatgcg tccggtactg gttcccccgg tactggttcc
     4621 cccggtaaaa attcgcccga tactgattcg tccggtactg gttccctcgg cgccgaggct
     4681 ttcatccgcg ccaataccgt cttggccacc gcccgccatg tgccggaaat ccgtctgcac
     4741 ctggccaccg agatcacgcc gatctggcag gccaccgagg cctggatggc cgagcacaat
     4801 gtcgagccgc cgttctgggc gttcgcctgg ccgggcggtc aggcactggc gcgtcacatc
     4861 ctggacaatc cctccctggt cgccggcaag cgtgtgctgg attttgccgc cgggggcggc
     4921 gtcgcggcca tcgcctgtgc cctggccggg gcggcgctgg tggaagcgtc cgagatcgat
     4981 cctctggcgc tgaccgccgt cggtctcaat gccgcggtca atggtgccgg agtgctccag
     5041 gcgggggacg tggtgggaga agcctgccgc tgggacctga tcctgtgcgg cgatgtctgt
     5101 tacgaggcgc cgatgacggg ccacatcatg ccgtggctga ggaaaatggc tggttcggcg
     5161 gaggtgtgga tcgccgatcc gaaccggtcc tacctgccaa agcaaggctt gctgcccttg
     5221 gcgcgctacc ggatcgagac gacgctggag ctggaagacc acctcagccg cgaggtgacg
     5281 ctttaccgcg tcctggccga gggctgaccg aacggcatgc cgttgatcgt caagggtccg
     5341 ccaccgaacg agatgtccca aacgacggta tcgccctgag cacgcccgat gcctttcgcc
     5401 atcaagatga aaggcaccac accctgcagg ttcggccggc cctgcatttg ggccaaagcg
     5461 gcatcgatgc cactcgccga aaggtggagg tcgacgccga tatctccgtc tgcgcgtggc
     5521 tggaaacgcg ccgaaccttt cacacgcacc ggcccggcgt cgaacgcgac cgactcgatg
     5581 gctgccttcg accccggtgc actcagaacg gcagccgctt gccgctgcag cgccgcctgg
     5641 tccgggttcg gcgcgatggc ggcctggagc aatgccatca atggccccag cggcacaccc
     5701 gtgagaaccg atcggacagc caggtgatgc ggcacgaacg aagccgggtc ggcagataga
     5761 ctggtcagcg aaaatccgtc gatcgccaga tcgaccgctg cgttga
//
LOCUS       DASWQN010000599         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2037673, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000599 DASWQN010000000
VERSION     DASWQN010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2037673"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1476)
                     /locus_tag="VGC82_10165"
     CDS             complement(73..1476)
                     /locus_tag="VGC82_10165"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEY0423675.1"
                     /translation="MILWFRDDLRLADQAALKAAIETGCPILPVFVLDDDAAGPWAPG
                     AASRWWLHHSLAALGQSLAERGNRLILRRGNSVKIIGELVEHSGATDVFTGGLVDPWA
                     RRVDKAVSDAVPARLHRMRTTTLFHPDSVRTKAGGAYTVYMPFAHASIALGGPKAPSP
                     APNAVRAAMPLHGDRLEDWNLLPTKPDWARGLRDTWTPGESGAMERAEAFLGNGLNPY
                     AAARDQPAGDGTSMLSPHLHFGEVSAPQLWHLAFRQPASNGRDIFVRELLWREFNANL
                     LWHNPDLPETPLRPKFANMPWRDDKPGMTAWQKGQTGIPIVDAGMRQLWRIGWMHNRV
                     RMIVASFLIKHLMIRWQDGEAWFWDTLVDADLANNANNWQWVAGCGADAAPYFRIFNP
                     VSQGKKFDADGDYVRQFVPELSRLDSRHIHAPWEAPADVLAKAGVTLGRSYPSPIVDL
                     AAGRARALDAFARIRGV"
BASE COUNT          311 a          576 c          542 g          294 t
ORIGIN      
        1 gaaggcctgc agcaggtcag cgaggataca gatccgatcg aagcctgaat ccagatcctg
       61 atgacaccgc tctcaaacac cgcgaatccg tgcgaaagcg tcgagtgcgc gggcccggcc
      121 cgcggcgagg tccacgatcg gacttggata ggatcgtcca agcgtcacgc cggcttttgc
      181 caggacatcg gccggcgcct cccacggcgc atggatgtgc cgtgagtcca gccggctcaa
      241 ctcgggcacg aactgacgga catagtcgcc gtcggcgtcg aacttcttcc cctgcgagac
      301 gggattgaag atccggaaat acggcgcggc atcggcaccg caaccggcca cccactgcca
      361 gttgttggcg ttgttggcga ggtcggcgtc caccagcgtg tcccagaacc aggcctcgcc
      421 gtcctgccag cggatcatca ggtgcttgat caggaagctg gcgacgatca tgcggacgcg
      481 gttgtgcatc cagccgatcc gccatagttg ccgcataccc gcatcgacga tcggtatccc
      541 ggtctggcct ttttgccagg ccgtcatccc cggcttgtcg tcacgccacg gcatgttggc
      601 gaatttgggc cgcagcggcg tttccggcag atcggggtta tgccagagca ggttcgcgtt
      661 gaactcgcgc cacagcagtt cgcgaacgaa gatgtccctc ccgttactcg cgggctgccg
      721 gaatgcaagg tgccagagtt gcggcgcgga aacctcaccg aagtgcaggt gcggcgacag
      781 catggatgtg ccgtccccgg caggctggtc ccgtgcggcg gcgtaggggt taaggccgtt
      841 ccccaggaac gcttcggccc gctccatcgc gccgctctcg ccgggagtcc aggtgtcgcg
      901 caagcccctt gcccagtccg gctttgtcgg cagcaagttc cagtcctcca gccgatcgcc
      961 gtgtagcggc atcgccgcac ggaccgcgtt cggggccggg gatggcgcct tcggcccgcc
     1021 aagggcgatg gaggcgtggg cgaacggcat atagacagta taggcgccgc cggccttggt
     1081 ccgtacggaa tccgggtgaa acaacgtcgt cgtgcgcatc cggtgcagcc tggccgggac
     1141 tgcgtcggac accgccttat cgacacgccg cgcccacgga tccaccaagc cgccggtgaa
     1201 aacgtccgtg gcgccgctgt gttcgacaag ttcaccgatg attttgacgc tgttgccgcg
     1261 gcgcaggatc aaccggttgc cccgttccgc caaggattgc ccaagggccg ccagactgtg
     1321 gtgcaaccac cagcgcgagg ccgcccccgg cgcccacggc ccagcggcat cgtcgtccag
     1381 gacgaacacc ggcaggattg ggcagccagt ctcgatcgct gctttcagag cggcctgatc
     1441 cgcgaggcga aggtcatcgc gaaaccacag gatcacgggc gggtcgtcat ttgttgtcat
     1501 agccacgact tggggatgtt ttggatcgtg tccacaatca gccgtcggac aaagcttcca
     1561 gcgctttggt gacccagcga gcaaaccacg gatagcgcgt ccggccgcac gaaatcaaaa
     1621 cgccattcgt gccggtccgt gcctgaatcc gcgcaatccg tgatgaacgc tttcttccca
     1681 cccgacgtcc cgccccacaa caagccccgt caacgcccct cag
//
LOCUS       DASWQN010000600         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2037679, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000600 DASWQN010000000
VERSION     DASWQN010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2037679"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VGC82_10170"
     CDS             complement(<1..254)
                     /locus_tag="VGC82_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0423676.1"
                     /translation="MKRILSGAAAGLLLVYAAFAADLPTAAPADVGIGRLDRITQMFK
                     ADTDAKRIPGAVVMLARDGKVVYHEAFGVRDPASGAPMQK"
     gene            complement(379..1500)
                     /locus_tag="VGC82_10175"
     CDS             complement(379..1500)
                     /locus_tag="VGC82_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423677.1"
                     /translation="MPDAGKAPGVASGFILAYRLFDVAYAIDLVQAEETWARTARTGS
                     SRGRLTTPPKAISFGEPPVAIGLGTVALALPDGSAQAAATARLFDFGVVSIALRVPVA
                     GLSWSAFTRRVNDMDRAVGVAPATVLWTELLDRVREGVGGALIRPIPVTLEEDYLVGV
                     ATAFSESIPAEALAARLDLVPLLSGEQRKLSEGARRDLLRHQYSYYTDDLVVLTWDRA
                     FIYEPRGDTDVIDVLEVANAQLLEMRYYDELLDAELPRMYDLVEATRRRWALPGARRF
                     ADLARRLYTMVAEVTELTEKVDNALQVTEDVYLARIYAAALELFRVPTVSAAVDRKLS
                     IIRDTYTALYDEAHSSRSEWLEIVILVLIAVEIVLAVVR"
     gene            complement(1508..1804)
                     /locus_tag="VGC82_10180"
     CDS             complement(1508..1804)
                     /locus_tag="VGC82_10180"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEY0423678.1"
                     /translation="MLFLVISEPRPERPSSVTESRQSYWTWIQPLIDGGEVRSVHAKV
                     GRGAVVLFDVASNEVLHAHLNAWADIIPARFDVHALIDPASARHFLAEQGAAAV"
     gene            complement(1952..>2875)
                     /locus_tag="VGC82_10185"
     CDS             complement(1952..>2875)
                     /locus_tag="VGC82_10185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423679.1"
                     /translation="SSRATELWHVVVLYGVIMTLGANCLGLVVFVPLLSRYFVRNRGM
                     AVSVVQSANGFARAFSAPMTTLMISGLGWRGAYLWQGAFMAVVLVPLAGLFRGIGQPS
                     HRRATEGEGWTVRQAVRTPHFWLLFAVYMCTGLGSFLVALHQLAFAVDIGFDKLYAAG
                     VLGMGAFLSLPGVIITGTLSDYVGRELSAVVTYGTSILGVVFALMITSPDQHVLLWLH
                     ACFFGLTWGARGPAITAKTADLFPGPNLGAILGVITIGTGLGAAGGSWMAGFVFDTTG
                     SYRVAFVLSIVFYVAGSIAFWALRRPPAIRA"
BASE COUNT          577 a          983 c          866 g          449 t
ORIGIN      
        1 tccttctgca tgggcgcgcc gctggcggga tcgcgtacgc cgaacgcctc gtggtaaacg
       61 accttgccgt cgcgggcgag catcaccacc gcgccgggga ttcgcttggc gtccgtatcc
      121 gccttgaaca tttgcgtgat ccggtccagc cggccgatcc cgacatcggc gggtgccgca
      181 gtcggcagat cggcggcaaa agcagcgtag accagcaaca gccccgccgc tgcgccagat
      241 aaaatgcgtt tcaccaacgt tcctccagtt ccgccagtac ctcggcgtgg gtgtaatcct
      301 cggcgtggat tttccggggt gcaaggatcg gccaggggtt cgccatccgc ttgtgcggtc
      361 cctgacgcaa aaggtgtctt accgaaccac cgccagcacg atctccaccg cgatcagcac
      421 caggatgacg atctccagcc attccgaccg gctgctgtgg gcttcgtcat acaacgcggt
      481 gtaggtatcg cggatgatcg acaacttgcg gtccaccgcc gcgctgactg tcggcacccg
      541 gaacaactcc aacgccgcgg catagatccg cgcgaggtaa acgtcctcgg tcacctgcag
      601 cgcgttatcg actttctccg tcagttccgt tacttcggcg accatcgtgt aaagccgccg
      661 agccaggtcg gcgaagcgcc gcgcgcccgg cagcgcccat cgccgccgcg tcgcctccac
      721 caggtcgtac atccgcggca gttcggcatc cagcagttcg tcataatagc gcatctccaa
      781 caactgggcg ttcgccactt ccagcacgtc gatcacgtcg gtatcgccgc gcggctcgta
      841 gatgaaagcc cgatcccagg tgagcaccac caggtcgtcg gtgtagtacg agtactggtg
      901 tcgcagcaga tcgcgccggg caccctcgga cagcttgcgc tgctcgcccg acagcagggg
      961 aaccaggtcc agccgcgctg ccagcgcctc ggcggggatc gattcactga acgcggtggc
     1021 gacgccgacc agataatcct cttccagcgt gaccgggatc ggccggatca gggcaccgcc
     1081 aacgccttca cggacgcggt ccagaagctc cgtccacagg accgtggcgg gcgccacgcc
     1141 gacagcacga tccatgtcgt tcacgcgccg ggtgaacgcc gaccaggaca ggccggcgac
     1201 cggaacgcgc agcgcgatgc tgacgacccc gaagtcgaac aagcgggcag tggcggccgc
     1261 ctgagcggaa ccgtcgggca gggcaagtgc gacggtaccc aggccgatgg cgaccggcgg
     1321 ttcgccaaac gaaatggctt taggcggagt cgtaaggcgc ccgcgggaac ttccggtacg
     1381 cgcagtccgt gcccaggttt cctcggcctg aacaagatcg atcgcataag cgacgtcgaa
     1441 cagccgatag gcaaggatga atcctgaagc gacacctggg gccttgccgg cgtccggcat
     1501 gacgggtcta aaccgctgcc gctccttgtt cggccaggaa atgccgtgcg cttgccggat
     1561 cgatcaatgc atgcacgtcg aaccgcgctg ggatgatgtc cgcccaggcg ttcagatggg
     1621 cgtgcagaac ctcgttcgag gcaacgtcga acagcacgac ggcgccgcgt cccaccttgg
     1681 cgtgaaccga ccgcacctcg ccgccatcga tcaacggttg gatccatgtc cagtagctct
     1741 gccgcgactc ggtcacgctg gatggccgct ccggcctggg ttcgctgatc accagaaaaa
     1801 gcatgcggca actccttgaa tggccggatg ggatatacgt tccacccgcc tagagcgaga
     1861 tccgtgttga tagccgcacg gcttccactc taacccttga tttcacgagc agctttatcc
     1921 ggtaggtggt cccgcttggt cggatgatgc tctaagcccg gatcgccggc ggacgccgca
     1981 gcgcccagaa tgcgatgctg ccggcgacat agaacacgat cgacagcaca aacgcgaccc
     2041 ggtagctgcc cgttgtgtcg aatacgaagc ccgccatcca cgatccgccc gcggcaccca
     2101 acccggtgcc gatggtgatc acccccagga tcgcccccag gttcgggccg ggaaacaagt
     2161 cggctgtttt ggcggttatc gccggccccc tcgcacccca cgtcaggccg aagaaacagg
     2221 catgcagcca gagcagcaca tgctggtccg gcgacgtgat catcaaagcg aacacgaccc
     2281 ccaggatcga cgttccatag gtgacgaccg ccgacagttc ccgcccgacg tagtccgaca
     2341 aagtcccggt tatgatcacg ccgggcaacg agaggaacgc acccatgccg agcacgccgg
     2401 cggcgtaaag cttgtcgaag ccgatatcga ccgcgaatgc cagttgatgg agcgccacga
     2461 gaaaactgcc caggccggtg cacatgtaca ccgcgaacaa cagccaaaaa tgcggagtcc
     2521 ggactgcttg gcggaccgtc cagccctccc cttccgtcgc ccgccggtgg ctcggctgtc
     2581 cgatgccgcg gaacagcccg gcgagtggca ccaggacaac ggccatgaat gcgccctgcc
     2641 acagatacgc cccgcgccag cccaggccgg agatcatcaa tgtggtcatc ggcgcggaaa
     2701 atgctcgggc aaatccgttc gccgactgga cgaccgacac cgccatgcct cgattgcgga
     2761 cgaagtaccg cgacagcaac ggcacgaaca cgaccagccc gaggcaattc gcccccaacg
     2821 tcatgatcac gccatacagc acgacgacgt gccacaactc ggtcgcacgg gaact
//
LOCUS       DASWQN010000601         5064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2565781, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000601 DASWQN010000000
VERSION     DASWQN010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2565781"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VGC82_10190"
     CDS             <1..261
                     /locus_tag="VGC82_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEY0423680.1"
                     /translation="ELRAELPDADPARLTDEVGDLLFVLANLARKLNLDPETCLRHAN
                     EKFSRRFNAMERDFEVRAQSLPELSLEEMEAGWQRVKAAEPG"
     gene            complement(342..926)
                     /locus_tag="VGC82_10195"
     CDS             complement(342..926)
                     /locus_tag="VGC82_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEY0423681.1"
                     /translation="MSDVATAEKTTTLPRTPPQILGPFYPFMQTPTDSGDLTNGGAAQ
                     GTILYLSGRVLAANGQPAVGAKVEIWQANAAGRYSHPNDDNLAAPIDEKFNGFAVTTT
                     DGQGRYSFKTVRPAAYPASPGRWRPAHIHFSVTAKYEQLVTQMYFAGDKWNETDSWLN
                     SASRKDLLITEPTPAVGKEPGAQAVIFDIVLTRG"
     gene            1061..1486
                     /locus_tag="VGC82_10200"
     CDS             1061..1486
                     /locus_tag="VGC82_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEY0423682.1"
                     /translation="MLNDAQRHFLDKMRVAHLATADRAGAPHVVPVCFCLNGSSLYIT
                     VDEKPKRTDVPLKRIRNIRENPQVAVTVDRWDEDWTRLAWVMVRGQADILTGGEEHAD
                     AQRQLCLRYPQYHTMDLKPLPVIAVRIQRVLSWGALAAC"
     gene            complement(1618..2133)
                     /locus_tag="VGC82_10205"
     CDS             complement(1618..2133)
                     /locus_tag="VGC82_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423683.1"
                     /translation="MTPPDQLKPRRPTSQGKPERAPWTAWLPVLAAAVIMITGCLSTF
                     LVLQLDEFRPKVGDIVAFKPGSQDGDMWQMTIPATLVSAIGIPAIGVPREACSLDPNV
                     MAEHGGSLIVEGLQDEPTLQYRIHWAGSATSRAANDCGKSASLVVSRTDLQRLANAAG
                     GFGVGEKGSPR"
     gene            2349..3359
                     /locus_tag="VGC82_10210"
     CDS             2349..3359
                     /locus_tag="VGC82_10210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0423684.1"
                     /translation="MRFGLIIRGQYPQGDDMRVRLKEDLDAARRAEALGYDLIAKGSH
                     YSSYPFQYIQQIPYLCQVALAAPKLRLCPGVVLLPLHSPLHVAEELGSLDVMCDGKLI
                     FGAGIGYREVEFNAFGSTQKQSGPRFEECLTAVKRLWTEDFVTMEGSYFKLDHANCTT
                     MPVQKPMPPVWIGANANVGIRRAARMADAWFINPHNKIDTIAEQMEVYKRALDACGKP
                     FPHELPMAREVFVAATRNEAIRLARPSLEAKYKAYKDWGQDKVMPTGDAFSVDFEELM
                     NDRFLFGSPSEVVEQILVLKRRFGVNTLILGIHWVGMPASLAMEQMQLISEEIFPAVR
                     SA"
     gene            complement(3398..4408)
                     /locus_tag="VGC82_10215"
     CDS             complement(3398..4408)
                     /locus_tag="VGC82_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0423685.1"
                     /translation="MKQRQLGRTGPLVSALGLGCMGMSGMYGNADRAESITTIHAALD
                     AGITLLDTGDFYGMGHNEMLIGEALRGVRRDNVVVSVKFGALRDAAGGWGGYDARPAA
                     VKNFLAYTLQRLGLDYIDIYRPARLDQNVPIEDTVGAIADSIKAGYVRHIGLSEVGAQ
                     TIRRAAATHPISDLQIEYSLISRGIEDQILPACRDLGIGITAYGVLSRGLISGHWHKG
                     AAGAGDFRAFSPRFQGENMDRNLARVDALRAVAEAKGVSVAQIAIAWVTAQGDDIVPL
                     IGARKRDRLAESLGALDVALSAADLAAIEQAVPKGAASGDRYPGAAMADLDSEAGRSS
                     PA"
BASE COUNT          931 a         1648 c         1559 g          926 t
ORIGIN      
        1 gagcttcgcg ccgaactgcc ggatgccgac ccagcgcggc tgaccgacga agtgggcgat
       61 ctgctgtttg tcctggctaa cctcgctcgc aagctgaacc ttgatccgga aacctgcctg
      121 cgccatgcaa acgagaagtt ctcgcgccga ttcaatgcga tggagcgcga cttcgaagtc
      181 cgggcccaaa gcttgccgga actgtcgctg gaggagatgg aagccgggtg gcagcgtgtg
      241 aaagccgccg aaccgggttg acgcccctgc ccgattcgcc ctctgcccgg ttcgccctct
      301 gcccggttcg cccgccggcc acccgcgaga cagacgccgc cttagccccg ggtcagcacg
      361 atatcgaaga tcaccgcctg cgcgcccggt tccttgccga cggccggcgt cggttcggtg
      421 atcaacaaat ccttgcggct cgcgctgttc agccaggaat ccgtctcgtt ccatttgtcg
      481 ccggcgaaat acatttgtgt gacaagctgt tcgtatttcg cggttaccga gaaatggata
      541 tgcgccggcc gccaacgccc gggggaggcc ggataggcgg cagggcgcac ggtcttgaag
      601 gaatagcgcc cctgcccgtc cgtcgtcgtt accgcgaacc cgttgaactt ctcgtcgatc
      661 ggtgccgcca gattgtcgtc gttcggatgc gaataccgcc cggcggcgtt cgcttgccag
      721 atctctacct tcgcacccac ggcaggctgc ccattggccg ccagcacacg tcccgacaga
      781 tacagaatgg tcccttgcgc cgcgccgcca ttggtcaaat cgccgctatc cgtgggcgtt
      841 tgcatgaacg gatagaacgg ccccaaaatc tgaggcgggg tacgcggcag cgtcgtggtc
      901 ttttcagcgg tggcgacatc ggacatcgtc gttctctccc cgaaagccgc cggatcacga
      961 catcatagcc gctcgccggc agactggata tttattgcat gatgcgcgcg ctcagaccac
     1021 ccgcccagcg cgatcatgtt ccaacgctaa tggtgacgcc atgctgaacg atgcgcaacg
     1081 gcatttcctc gacaagatgc gggtggccca cctcgcgacg gcagaccgtg cgggagcgcc
     1141 gcatgtggtt ccggtctgct tttgcctgaa cgggtcgtcc ctctacatca ccgtggatga
     1201 gaagccgaag cgcacggatg tcccgctcaa gcgcatccgc aacatccggg agaatcccca
     1261 ggtggcggta accgtcgatc gctgggatga agactggacc cggcttgcct gggtgatggt
     1321 gcgaggccaa gcggacatcc tgaccggcgg agaagaacat gccgacgcgc aaaggcagct
     1381 ttgcctgcgc tacccgcagt accacacgat ggacctgaag ccgcttccgg ttatcgctgt
     1441 gcggatccag cgggtccttt cgtggggagc gttggccgcc tgctagagcg tgatcacgct
     1501 ggacggaatg tccaaagaat atgaatcatg cgaccagaca agaggataga acattttcaa
     1561 cctgtacagt caggctgaaa ttccgctaac ctccgcattc gccgctgcgt cagggcgcta
     1621 acggggactt cccttttcac cgacgccgaa accgcccgcg gcgttggcca atcgctgcaa
     1681 gtccgtgcgc gaaaccacca gactggcgga tttaccgcaa tcattggctg cgcgggacgt
     1741 cgccgaaccg gcccaatgga tgcggtactg cagcgtgggc tcgtcctgca gaccctccac
     1801 gatcaggctg ccgccgtgtt cggccatgac attcgggtcg agcgaacaag cctcccgcgg
     1861 cacgccgatc gccggaattc caatcgccga gaccagcgtc gccggaatgg tcatctgcca
     1921 catgtcgcca tcctgcgacc cgggcttgaa cgcgacgata tcacctacct ttggccggaa
     1981 ctcatcgagt tgcaggacca aaaaggtgct gaggcatccg gtgatcatga tcaccgccgc
     2041 tgccaggact ggcagccacg ccgtccacgg cgcccgttca ggctttcctt gcgaggtggg
     2101 gcgtctgggt tttagctgat ccggtggtgt catggcttcg tagccctctt cacggaagcg
     2161 gatgtcgact gctatccgtc atattgtgca gcgcaccata aaagcaagct ttccgttcgg
     2221 ccttcgtgtc gatctccatc tggccgcctt gacagcctat ctggccgcct gtccgtcacc
     2281 cgtgaagatg tccgccttga cagcatcgaa cgcgatgcca cgctcccgga acgcgaagga
     2341 ggaaacccat gcgcttcggc ctgatcatcc gcggccagta tccgcaaggc gacgacatgc
     2401 gggtccgcct gaaggaggac ctcgacgccg caaggcgtgc cgaggcgctc ggctacgacc
     2461 tgatcgcgaa aggctcgcat tacagttcgt atccgttcca gtacatccag caaatcccct
     2521 atttgtgtca ggtcgcgctg gcagcgccga agctgcgcct gtgtcccggc gtcgtgctgc
     2581 tgcccctgca cagccccttg catgtggcgg aggaactcgg ttcgctcgac gtcatgtgcg
     2641 acggcaagtt gatctttggt gccggcatcg gctaccgcga ggtggaattc aacgccttcg
     2701 gatctaccca aaagcaatcg ggcccgcgat tcgaggaatg cctgaccgcg gtaaagcgcc
     2761 tgtggacgga agatttcgtc accatggagg gcagctactt caagctggac cacgccaact
     2821 gcacgacgat gccggtgcag aagccgatgc ctccggtctg gatcggcgcc aatgctaacg
     2881 tcggcataag gcgggcggct cgcatggcag acgcctggtt catcaatccg cacaacaaga
     2941 tcgacaccat cgccgaacag atggaggtct ataagcgcgc tctcgacgcc tgcggcaaac
     3001 catttcccca cgaacttccc atggctcgcg aggtgttcgt ggcggccacg cggaatgagg
     3061 cgatccgcct cgcgcggccg tcactggaag ccaagtacaa ggcctacaag gactgggggc
     3121 aggacaaggt gatgcctacg ggggacgcgt tcagcgtgga cttcgaggag ttgatgaacg
     3181 accgtttcct gttcggttcg ccgagcgaag tcgtggagca gatcctggtg ctgaagcgcc
     3241 gtttcggtgt caacacgctg atcctcggca tccattgggt cggtatgccg gccagcctgg
     3301 cgatggaaca gatgcagctt atctccgagg agatattccc ggcggtccgg tcggcctgat
     3361 cggcaccaac cggaccttgc cttgagtcaa cggccggtca tgcgggcgag gaacgcccgg
     3421 cctcgctgtc caggtccgcc atggctgcac cggggtagcg atcgcccgat gcggctccct
     3481 tcggcaccgc ctgctcgatc gccgccaggt cggcggctga cagcgcgacg tccaacgcgc
     3541 ccagggactc ggctaaccgg tcacgttttc gagcaccgat caacggcacg atatcgtcgc
     3601 cttgtgcggt gacccaagcg atcgcgatct gcgccacgct gacgcccttg gcctcggcga
     3661 ccgcacgaag cgcgtccacc cgcgccaggt tccggtccat gttctcgccc tggaagcgcg
     3721 ggctgaacgc gcggaagtca ccggcgcctg cggcgccctt gtgccaatgt ccgctgatca
     3781 gcccgcgcga caatacgcca tacgcggtga tcccgattcc caggtcgcgg caggccggca
     3841 ggatctggtc ctcgatccca cgggagatca gcgagtattc gatctgcagg tcgctgatcg
     3901 ggtgcgtcgc ggcagcccgg cggatagtct gcgcgcccac ctcggacaat ccgatatggc
     3961 ggacatagcc ggctttgatg gagtcggcga tggcacccac ggtgtcttcg atcggcacgt
     4021 tttgatcgag ccgggcgggg cgatagatgt cgatatagtc gagacccagc cgctgaagcg
     4081 tgtaggcgag gaagttcttc accgcggccg gccgcgcatc ataaccgccc caaccgccgg
     4141 ccgcgtcgcg caacgcgccg aacttgacgc tgacgactac gttatcgcgc cggacgccac
     4201 gtagggcctc gccgatcagc atctcgttgt ggcccatgcc gtagaagtca ccggtatcga
     4261 gcaatgtgat cccggcatcc agggccgcat ggatcgtggt gatgctttcc gcccgatcgg
     4321 cgttgccata catgcccgac atgcccatgc agccgaggcc aagcgcggaa accaatgggc
     4381 cggtgcgacc gagctgacgc tgtttcataa gttacctccg tgggaagtga gtgacgggaa
     4441 cttaagtgcc tgatgataaa caaccgcttc gaaccgggcg aacttccgca gcaatgtcat
     4501 ggtgggcgca ggcccaccac cacggcccgc ggtatgcgat ctggcgaagg cgtggatggt
     4561 gagcctgcgc cctccatgac gatatccgcg acctcgcccg aagcggttga cgcgattcgg
     4621 cccctgaccc gatgaggcgg tgcggtgtcc ggaagaccgg ctcgatatcc gcccgctcgg
     4681 ccactcgcag cattagcgtc gtcgacgcca acgtccggct gttgcgccga tcggttccgg
     4741 ctttgtcaga ccgcagcccg ctcagggtgc gggccttcga ccgcttctgg agtcagaaac
     4801 cgtgcggaac gcgccggctg ggtcgcgtcc gctgcaaccg gcaagttccg ccgatctggt
     4861 ggcggatcga cgccaccgtt tccggccaat cgacatggcg acggcgcctg cccacgccat
     4921 tgacctaatc atgcgaagtg gcccgccgcg ctgcgggagg ccacgccgcc gggcaggcta
     4981 cttcttgacg gcgaagaccc acacgacacc gccttgcggc acattcggat ccagtttcag
     5041 gctggtcttc gccagcatgt cctg
//
LOCUS       DASWQN010000602         3951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2433274, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000602 DASWQN010000000
VERSION     DASWQN010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2433274"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VGC82_10220"
     CDS             <1..511
                     /locus_tag="VGC82_10220"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0423686.1"
                     /translation="GQSLRRVFELFRPDMDFANVRKLRAASGWTYDFVAQDLYPDAIP
                     CLTALRERGYKVLIAGNQPLESEAALARLDVPADLIASSAGWGVSKPDPRFFDKVIEV
                     AGEAPGSIAYVGDRLDNDVLPTLTAGMKAVFLRRGPWGWMHAQMPEIERAHIRLESLL
                     DLPDRLATL"
     gene            508..933
                     /locus_tag="VGC82_10225"
     CDS             508..933
                     /locus_tag="VGC82_10225"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0423687.1"
                     /translation="MTSYDPAADGWRRLPGAPLPCGLGVPWAKRLDGLWHYGLATTAE
                     HGNNNGVVHGGVLMAFADHGLSLLAWEAAQRAPCTTIQLNTHFMNAITPGEFMELRGE
                     VTRRTPGLVFVRGLIGIREDKAVRDVGAVDGIWRVLRSR"
     gene            988..1743
                     /locus_tag="VGC82_10230"
     CDS             988..1743
                     /locus_tag="VGC82_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0423688.1"
                     /translation="MDIDHEPSRVPGPLTLAIDVGGTRLKVGLLDWNGTMVAGPNRVE
                     TPRHPAPAVVVDALVNLAAPFGHFDRISVGFPGVVRNGQVRTAPNLGTPEWRDFPLVA
                     TLAKKLGKPARMLNDAEVQGLGVISRRGLECVITLGTGMGFSLFQDGHPAPHLELSQH
                     PIHKGKTYDQFIGVAALKAIGRPRWNRRLRRVFASITTLVGFDTLYIGGGNAKHVEVE
                     LAPNMRIVPNEAGIIGGVRLWDKELDPVFAAML"
     gene            complement(1755..2336)
                     /locus_tag="VGC82_10235"
     CDS             complement(1755..2336)
                     /locus_tag="VGC82_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0423689.1"
                     /translation="MYTFYHAPGSSSLAVHIALNEVGAAFDGRPISFAHRDRDPAEFR
                     TMNPEGKVPTLVIDGRPLTEVAAILYYLARTYPEALLFPAGDIEAEAQVISWMSFTAS
                     TLHPARQRGGEHAQTVYLLAERRLGAKTWAAGAYSIADIHLFRLFWRFRGSLGANPET
                     FPNLTSHLNRMMERPAVRKTFAAEAALGYDLPA"
     gene            complement(2346..2756)
                     /locus_tag="VGC82_10240"
     CDS             complement(2346..2756)
                     /locus_tag="VGC82_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0423690.1"
                     /translation="MVENVAPTQTWEALLADADAALVDVRTDAEWNFVGVPDLSEAGK
                     ETVLIPWQIYPMMQRNSSFEDNLKQAGLTADSKIYFICRSGVRSMAAAEAAKAAGFPH
                     VFNVADGFEGPPDAEGHRGGTAGWKADALPWRQR"
     gene            2787..3806
                     /locus_tag="VGC82_10245"
     CDS             2787..3806
                     /locus_tag="VGC82_10245"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY0423691.1"
                     /translation="MTASYHGTTWQDDLAQAIGNTPLIKLRRASEATGCTILGKAEFM
                     NPGGSVKDRAGLAIITDAEQRGTLREGGTIVEGTAGNTGIGLTLVGNARGYRSVIVMP
                     ETQSQEKIDFLRMIGADLRLVPAKPYRDPGNYVHVSRRLAEELAAETGNAIWANQFDN
                     LANREGHRITTGQEIWDQTGGRIDAFTCACGTGGTLAGVSLALKARNPDVRIVLADPL
                     GSGLYGWVKSNDLSIEGSSITEGIGQSRVPDNLSGVEIDDAERIPDAEALEQIFEVLI
                     HEGLSVGTSSGINIAAAIRVAKAMGPGHTIVTILCDGGSRYQSKLFNPEFLRGKGLPV
                     PPWME"
BASE COUNT          716 a         1252 c         1292 g          691 t
ORIGIN      
        1 cggccaatcg ctgcgccgcg tgttcgagct gttcaggccc gacatggact tcgccaacgt
       61 gcgcaaactg cgtgccgcct ccggctggac ctatgatttc gttgcccaag acctttatcc
      121 ggacgcaatc ccgtgcctga ccgcgcttcg cgaacgcggc tacaaggtgc tgatcgccgg
      181 gaaccagccg cttgaatccg aggcagccct cgcgcggctg gatgtcccgg ccgatttgat
      241 cgccagttcc gccggttggg gcgtctcgaa acccgacccg aggttcttcg acaaggtgat
      301 cgaggttgcc ggcgaagcgc cgggctccat cgcctatgta ggcgatcggc tggacaacga
      361 cgtgctaccg accctgaccg ccgggatgaa ggcggtcttc ctgcgccggg gcccctgggg
      421 ctggatgcat gcgcaaatgc cagagatcga acgcgcccat atcaggcttg aaagcttgct
      481 cgacctaccc gaccggctgg caacactttg accagctatg atccggcagc cgacggatgg
      541 cgccggcttc ccggcgctcc gctgccatgc ggcctgggcg tgccttgggc gaagcggttg
      601 gacggcctgt ggcactacgg cctggccacc actgctgagc acggtaacaa caatggtgtc
      661 gtccacggcg gcgtgctgat ggcattcgcg gaccatgggc tgagcttgct ggcctgggaa
      721 gcggcgcagc gggcaccatg cacgacgatc cagctcaata ctcatttcat gaatgccatt
      781 acgcccggcg agttcatgga attgcgcggc gaggtcacgc ggcggacgcc gggccttgtt
      841 tttgttcgcg gcctgatcgg cattcgagag gacaaagcag tgcgggatgt cggcgctgtt
      901 gacggcatct ggcgggtgct aagatcgcga tgaggtcgca gcccgacggc tgcttcccgc
      961 gcaacggtcc ccagggaggg atgatccatg gacatcgatc acgaaccatc acgggtgccg
     1021 ggtcctttga cccttgccat cgacgtcggt ggcacacgac tgaaggtcgg cttgctcgac
     1081 tggaatggta ccatggtcgc cgggcccaac cgggtggaga cgccgcggca tccggctccg
     1141 gccgttgtcg tcgatgcgtt ggtcaacctg gcggctccgt tcggccattt cgaccggatc
     1201 tcggtaggtt ttcccggggt cgtgcggaat gggcaggtgc ggacagcgcc caatctgggc
     1261 acgcccgagt ggcgcgattt tccattagtg gccactctgg cgaaaaaact cggcaaaccg
     1321 gcacgcatgt tgaacgacgc cgaagtgcag gggctgggcg tgatcagccg aaggggcctg
     1381 gaatgcgtga tcacgctggg caccgggatg ggcttctcgt tgttccagga tggccatcca
     1441 gctccgcatc tggagctgag ccaacacccc attcataagg gcaagaccta tgaccagttt
     1501 atcggcgtgg ccgccctgaa ggcgatcggc cggccgcgct ggaaccgccg gctgcggcga
     1561 gtgttcgcgt ccatcacaac gctggtcggt ttcgacacgc tctacattgg cggcggcaac
     1621 gccaagcatg tggaggtgga acttgccccc aacatgcgga tcgttccgaa cgaggcaggc
     1681 atcatcggcg gcgtgaggct ttgggacaag gaactggacc cggtcttcgc agcgatgctc
     1741 tgaccgcgcc cacttcaggc aggtagatca tatcccagcg cagcctccgc ggcgaaggtc
     1801 ttgcggacgg cgggtcgttc catcatccgg ttgagatgcg aagtcagatt aggaaacgtc
     1861 tcgggattcg cacccaagga tccccggaag cgccagaaca ggcggaacaa gtggatgtcg
     1921 gcgatcgaat acgcgcccgc tgcccaggtc ttcgcgccca gccgccgttc ggccaaaaga
     1981 tacaccgtct gcgcgtgctc gccgccgcgc tgccgagcgg gatggagcgt ggaggcggtg
     2041 aaggacatcc aggagatcac ttgcgcctcg gcttcgatat cgcctgcggg aaacagcaag
     2101 gcttctgggt atgtccgggc cagatagtaa aggatggcgg cgacttcggt cagcgggcgg
     2161 ccgtcgatga ccagcgtcgg gaccttgcct tccgggttca tcgtgcggaa ttccgccggg
     2221 tctcgatcgc ggtgagcgaa agaaatcggc cgcccgtcaa acgccgcacc gacttcgttc
     2281 agcgcgatgt gtaccgccaa cgagctcgac ccgggggcgt ggtagaacgt gtacatcgcc
     2341 gcttcctacc gttgccgcca gggcagcgca tcggctttcc aaccggctgt gccgccgcga
     2401 tggccctcgg catcaggggg cccttcaaat ccatccgcga cgttgaacac atgcgggaaa
     2461 ccggcggcct ttgcggcctc ggctgctgcc atactgcgga caccgctgcg gcagatgaag
     2521 tagatcttgc tgtccgccgt cagtccggcc tgcttcaggt tgtcttcaaa gctgctattc
     2581 cgctgcatca tcggatagat ctgccagggg atcaggaccg tctctttgcc ggcttcgctt
     2641 aggtcgggca cgccgacgaa gttccactcc gcgtctgtcc ggacatcaac caaggcggcg
     2701 tctgcatccg caagcagggc ttcccaggtt tgagtaggcg cgacattctc aaccatgctg
     2761 aacgctccgg tgggaaagag aactttatga ccgcttcata ccacggtacc acctggcagg
     2821 acgacctggc ccaggcgatc ggcaatacgc cgctgatcaa actgcggcgc gcctcggagg
     2881 cgaccggctg cactatcctg ggcaaggcgg agttcatgaa cccgggcgga tcggtcaagg
     2941 accgcgccgg cttggcgatc atcaccgacg ccgaacaacg cgggaccttg cgcgaaggcg
     3001 gcaccatcgt cgaggggacc gccggcaaca ccggcatcgg cctgaccctg gtcggcaacg
     3061 cccgcggcta ccgcagcgtg atcgtgatgc cggaaaccca atcgcaggag aagatcgatt
     3121 tcctccgcat gatcggcgcc gacctgcgtc tggtgccggc caagccgtac cgcgaccccg
     3181 gcaactacgt ccacgtttcc cgccgtctgg ccgaggaact cgccgctgaa accggcaatg
     3241 ccatctgggc caaccagttc gacaacctgg cgaaccggga gggccatcgc atcacaaccg
     3301 ggcaggaaat ctgggatcag accggcggca ggatcgacgc gttcacctgc gcatgcggca
     3361 ccggcggcac gctggctgga gtctcgctgg cactcaaggc gcgcaatcct gacgtccgca
     3421 tcgtgctcgc cgatccgctt ggctccggcc tgtatggctg ggtcaagagc aacgacctgt
     3481 cgatcgaggg atcgtcgatt actgagggca tcggtcagtc acgcgtaccg gacaatctct
     3541 cgggcgtgga gatcgacgac gccgaacgca tcccggacgc ggaagcgctg gagcagattt
     3601 tcgaggttct gatccacgag gggctttcgg ttggcacctc gtccggcatc aacatcgccg
     3661 ccgcgatccg ggtcgcgaag gcgatggggc cgggccacac gatcgtgacg atcctgtgcg
     3721 acggcgggtc gcgctaccag tcgaagctgt tcaatccgga gtttctgcgg ggaaaggggc
     3781 taccggtccc gccgtggatg gagtgaggcc ggctatctgg atcgcgggcg accttccgcg
     3841 gacgaccgcc gcgcaagcgc gcgcaggcaa atggcttcga actgggctgc cacacgcgac
     3901 caggagaacc gctcctccat caagcggcgt gccgcgcagg cgatacgggc g
//
LOCUS       DASWQN010000603        10022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2301676, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000603 DASWQN010000000
VERSION     DASWQN010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2301676"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VGC82_10250"
     CDS             complement(<1..204)
                     /locus_tag="VGC82_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0423692.1"
                     /translation="MKRRNMLKSAAMSAVLASPRISRADGAKTVTFAPHADLASLDPV
                     WTTADITRNYSLAVFDTLYGYDAT"
     gene            complement(267..1121)
                     /locus_tag="VGC82_10255"
     CDS             complement(267..1121)
                     /locus_tag="VGC82_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0423693.1"
                     /translation="MTLDLSPLVAEPGTNSLPYFDPAFPDQMLVLHAARPHAWHPGMP
                     VLFVHHGVARNGRDYRDYWLPHVDSAGLLAIAIEFPEASFPEYLWYNFGNLHAKDGSR
                     NPREQWTFGIDTRLFEALRDQGITTAPRYGLFGHSAGGQYVHRMLSFGYRDRVAVAIT
                     ANAGTYAMPDLATDWPWGLGATEVTDDDLRTLLTFPLTIMAGTADTKTTGRFFPKGPK
                     SLKQGPTRHARAHAYLRVGQATAADLGVSVAWKVIDVPDVGHDGRRMSDAAGPVIAEA
                     LSRSVTTP"
     gene            complement(1540..1824)
                     /locus_tag="VGC82_10260"
     CDS             complement(1540..1824)
                     /locus_tag="VGC82_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423694.1"
                     /translation="MRLSRPSANFSTARCAWDAILIRMDYIGDMTTYTIIPTPDGSGF
                     NIGIAGSNGARQTMLGFTSEAEAEAWINQDKRRDGTPQSHASSQAASPPG"
     gene            complement(1879..2841)
                     /locus_tag="VGC82_10265"
     CDS             complement(1879..2841)
                     /locus_tag="VGC82_10265"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY0423695.1"
                     /translation="MTTPVMEAAGLSKHFPARRGIFGGDRSVVRAVDGISFVIEQGRT
                     LGVVGESGCGKTTAARLVLGLEVPTSGAMRFDGHDLATLDKAGRRRYRKSVQAVFQDP
                     FASLSPRMRISAIIAEPLVTNETVTSAQVRHRVLELLDLVGLPQRSADLFPHEFSGGQ
                     RQRIAIARALSLSPKLVVLDEPVSALDVSIRAQILNLLSDLQERLGLAYFFIAHDLAA
                     VAHMSHTIAVIYLGRIVEYGDAHQVANDPRHPYTQALFSASLPAHPDERREDIVLTGE
                     VPSPLRPPSGCRFHPRCPSAMALCAREEPVLAEVAGRQVACHLY"
     gene            complement(2838..3857)
                     /locus_tag="VGC82_10270"
     CDS             complement(2838..3857)
                     /locus_tag="VGC82_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0423696.1"
                     /translation="MTAKPLLSLENLSAHYVSQKGTRVVPAVDDVTLRLNAGETLGIV
                     GESGSGKSTLALTILRLLPKAGRIVSGRMMFDGEDLLEKTDEQMRAVRGKRIAMILQD
                     PMASLNPLFTIGDQVGEPVRVHEGASRAGAWKRAVELLKSVRIPSPEKRVTQYPHEMS
                     GGMRQRIVGAIAISCGPRLLIADEPTTSLDPTIQAQYLSLLSDLQRRHGLAMIFITHN
                     LGIVARMCDQIAVMYAGRVVEHGPVRDIFNAPAHPYTKALLNSIPRMSDAHSRLAAID
                     GQPPDLSAMPPGCSFAPRCTAAVARCHVQEPPEFQIAEKRIARCWLVEPGAKTRPELV
                     GSNAA"
     gene            complement(3867..4760)
                     /locus_tag="VGC82_10275"
     CDS             complement(3867..4760)
                     /locus_tag="VGC82_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0423697.1"
                     /translation="MSITTHRISVAAAPSRSFAFWRLDGFPLIPILILSLILFVAVFA
                     DFLAPYDPQVGSLAHRFRPPAWQQGGSMKYLLGTDHVGRDVLSRLIFGARVSMIVGTA
                     AVLVAGSIGTLLGIVSAYLGGWVDQVIMRITDTWLALPALTFAIFLAAVVGPSELNII
                     IILGGVYWTRYARVIRGEVLSLKEREFVRLAIVAGCSKRVIMWRHILPNVINSAIVLA
                     TLMLGVVVVTEASLSFLGVGVPPPKPAWGLMLADGKKGLMVGYWWLTVLPGMCIMLMV
                     LSANLLGDWLRVKLDPQLRQL"
     gene            complement(4763..5683)
                     /locus_tag="VGC82_10280"
     CDS             complement(4763..5683)
                     /locus_tag="VGC82_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0423698.1"
                     /translation="MQQYIVRRVGYSFLSLFLLSLTIFLFVRLTGDPASLMAEPGASA
                     ADLAAIRVQFGLDQPLWVQYWHFIASFVRGDLGDSFYYRTPVLELYFQRLPNSLLLAG
                     AAMIFSLLIGIPSGVLAAVNVGRLWDSVGKVFALLGLSLPSFWVALVMILFFSVYLGW
                     LPSSGSGTIWHLLMPAFALGWYFAAAHMRLTRSSMLDVLGSEYVKLARLKGLPERLVI
                     GKHAFKNALLPVVTLAGINLVVMINVAVVVETVFAWPGIGRLLYEGITFRDFPIVQGV
                     VVIGGAMIVAVNLIVDILYALIDPRIRLES"
     gene            complement(5797..7338)
                     /locus_tag="VGC82_10285"
     CDS             complement(5797..7338)
                     /locus_tag="VGC82_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0423699.1"
                     /translation="MNPSTTRRGVLGLSAATMFGATTWPAAADGATLTIGAHISLAPT
                     WFDPAETAGLITPFMLMYAMHDAVAKAMPGNSMAPSLAESWTMSPDGLSHEFVLRQGV
                     KFHNGETMTSDDVKFSFERYRGASHRLMQDRVAAIETPDPARVRFRLKAPWPDFLTFY
                     AAASGAGWIVPRKYIEKIGDEAFKKAPVGAGPYKFLSFIPGVELVLEAFDGYWRKKPS
                     IKRLVFKVIPDEATRLAALQRGEVDIALSIRGELAEELSRAPGLSIKPAVGSAPYWLY
                     FPQQWNPKSPWSDYRVRQAVNLAIDRKTMNEALTLGHSHLTGSAFPENFDFYWQPPAP
                     VYDLAQARKLLSDAGFPSGFDAGDYHCDSSYANIGEVAINNLQELGVRATLRPLERAA
                     FIKAYSEKKLKDIVQGASGAFGNCATRTEEFFVKGGTYAYGNYPDIDALFAEQASEMD
                     RAKRTSVLHRLQQLAHEKTIYVPIWQLAFLSGVGPRVDQSGFGLIKGFVYPAPYEDLT
                     LKARA"
     gene            complement(7354..8919)
                     /locus_tag="VGC82_10290"
     CDS             complement(7354..8919)
                     /locus_tag="VGC82_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0423700.1"
                     /translation="MHLYRDSNDILVTRRALLGVTALGVLAAETMPAVAERNNRLVYA
                     AHISLAPTWFDPAEVSGIITPFMLLYALHDALVKPMPGNPAEPCLAEACTGSPDGLSY
                     EFKLRTGAAFHNGEPVTAEDVKFSFERYRGNAATFLKNKVKAVETPDPQRVIFRLRQP
                     WPDFLIYYAGVTGAGWIVPKKYVQSIGEEAFKKAPIGAGPYKFASFNPGVELVMEAFT
                     SYWRKTPAVKTLVWKVIPDETTRLAALKRNEVDVAYSIRGELAEELRDTPGLTLKPIV
                     IHSPFWIYFADQWDPKSPWHDVRVRQAVSLALDRVGISEALTLGYSPITYSIVPKDYD
                     GYWQPPKIPYDPSGARKLLAEAGFPNGFDAGEYYCDSSYANLGEAVVNNLAEVGLRVK
                     LRPIERAAFNKGYAEKRYRNLVQGGSGAFGNAATRMETFVVKDGAYVYGTYPDIDQLF
                     ADQASELDRAKRTAILHRMQQLLHERTIYAPIWQLAFLNGQGSRVEESALGLIGGHPY
                     SAPYEDLRLKPGV"
     gene            complement(9436..>10022)
                     /locus_tag="VGC82_10295"
     CDS             complement(9436..>10022)
                     /locus_tag="VGC82_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY0423701.1"
                     /translation="MTTHAAIASNPEIKAKIPGLQHQASLIGDEAVRHRGTMGGSLAN
                     NDPAACYPSALLALGGTIKTDRRSIAADDFFQGMFTTALEQGEIITSIELPIPEKSAY
                     EKFRNPASRYAIVGVFVAKFPSGVRLAITGASQGGVFRHTEMEKALSANFAPDAINGI
                     VTPATDMNADIHASAEYRAHLVGVISKRAVAKAA"
BASE COUNT         1961 a         3079 c         3136 g         1846 t
ORIGIN      
        1 tgtggcgtcg tagccgtaca gcgtgtcgaa aaccgccagc gagtagttac gcgtgatgtc
       61 cgccgtggtc cagaccgggt ccagcgacgc cagatccgcg tgcggcgcaa acgtaactgt
      121 tttcgctccg tcggctcggg agatccgcgg tgaagcgagg acggccgaca tcgccgccga
      181 cttgagcata ttgcgtcgct tcattcggat gcccccgatc tggttagaaa caggttaggc
      241 cgtgatcggc aaccaatcca gcagtatcac ggggttgtca cgcttcgaga cagtgcctcg
      301 gcgatcaccg ggcccgcggc gtcggacatg cggcgaccat cgtgacccac atcgggcacg
      361 tcgatcactt tccatgccac ggaaaccccc aggtctgccg cagtcgcttg tccgacgcgg
      421 aggtaggcgt gggcgcgggc gtggcgggtc gggccctgtt tcagcgactt tggtcccttg
      481 gggaagaacc gccccgtggt cttggtatcg gcggtgccgg ccatgatcgt cagggggaac
      541 gtcagcagtg ttcgcaggtc gtcatcggtt acctcggtgg cgccaaggcc ccaaggccag
      601 tcggtcgcga gatccggcat cgcataggtg ccggcgttgg cggtgattgc aacggcaacc
      661 cggtcgcggt agccgaacga cagcatccgg tggacgtact gccccccggc ggaatggccg
      721 aacaggccat agcgcggcgc ggtggtaata ccctggtcgc gcagcgcctc gaacagccgc
      781 gtgtcgatcc cgaaggtcca ttgctcgcgc ggattgcgtg aaccgtcctt cgcatgaagg
      841 tttccgaaat tgtaccagag gtactccggg aagctcgctt cgggaaattc gatcgcaatc
      901 gccagcaaac cagcggagtc cacgtgcggc agccagtagt cgcggtagtc gcggccgttg
      961 cgagcgacac cgtggtgcac gaacaacacc ggcatgcccg gatgccatgc gtgcggcctc
     1021 gcggcatgca gaaccaacat ctgatccgga aacgccggat cgaagtaggg cagcgaattc
     1081 gtccctggtt cggctacaag cggggacaga tcgagtgtca tgcgatcatg gtttccgttt
     1141 gcatcgcgtg atgaaggcac gccgaatgtt ccatcctgct tgatcgtgct tttgaaaaag
     1201 cattgtacct tacggtatcg ccttcacaag cagcgcttgg taagctgacg ttggtaccgt
     1261 atcggtggcc tcgcacacgg atagcctgca acaggatgga gagcgtcatt atagttgaag
     1321 ccggacgatc attcgtggcg ctgcgcaagg acggcccggc ttttgctgcc ggtcgcgcgc
     1381 cgatgtatca cgcggttacc ccgcactgcc gggtcgcgtt atgcgcaacc gaaccagggg
     1441 cgcggtccgc ctgggcggag ccaccgtcta ccaccgtcac ctgccgggcg tgcctggaga
     1501 aactggcccg tttgcgacgc gaacgccagc agaactgaat tatccgggcg gcgaagctgc
     1561 ttggctcgac gcatgtgact gcggtgtacc gtcgcggcgc ttgtcctggt tgatccatgc
     1621 ctcggcttcg gcttccgagg tgaaacccag catggtctgc cgagcaccat tgctgccggc
     1681 aattccgatg ttgaacccgg aaccgtccgg tgtggggatg atcgtgtatg ttgtcatgtc
     1741 ccctatatag tccatgcgga tcaggattgc atcccaggca catctggcgg ttgagaaatt
     1801 cgccgatggc cgagacagcc gcatggcact ggttcgatcg cgacgaccgg attcgtctcc
     1861 ccgcgtagcg gtagctcgtc aatacagatg acatgcgacc tggcgccccg cgacctccgc
     1921 cagcacgggt tcttcccgcg cgcatagcgc catggcactg ggacagcgcg gatgaaaccg
     1981 gcagcccgaa ggtggcctaa gcgggctcgg cacttcgccg gtcaacacga tgtcctcccg
     2041 ccgttcgtcc gggtgggccg ggagcgaagc ggagaacaac gcctgggtgt agggatgccg
     2101 cggatcgttg gccacctgat gagcgtcacc gtactcgacg atcctgccaa gataaatcac
     2161 ggcgatggtg tggctcatgt gcgcgacggc cgcgaggtca tgggcgatga agaagtacgc
     2221 cagtcccaat cgttcctgca ggtcgctcag caggttcagg atctgggcgc ggatcgagac
     2281 gtccaacgcg gaaaccggtt catccagcac caccagcttc ggcgacagcg acagcgcccg
     2341 ggcgatcgcg atgcgttggc gttggccgcc ggaaaactcg tgcggaaaca gatcggcgga
     2401 tcgttgtgga aggccgacca gatccagcag ttcgagtacc cgatggcgca cctgagcgga
     2461 ggtgaccgtc tcgttggtga ccagcggctc cgcgataatg gcgctgatcc gcatgcgcgg
     2521 gctgagcgag gcaaagggat cctggaacac cgcctgcact gacttgcggt accgccgccg
     2581 ccccgcctta tcgagcgtcg ccagatcgtg gccgtcgaag cgcatggcgc cggaggtggg
     2641 cacttccagg cccagcacca gccgggccgc cgtcgtcttg ccgcagcctg actcaccgac
     2701 aacgcccagg gtcctgccct gctcgatgac gaaagagatg ccgtccaccg cgcgcaccac
     2761 ggaccggtcg ccgccgaaga tgccgcggcg cgccggaaag tgcttactta gtccggcggc
     2821 ctccatgaca ggtgttgtca tgccgcattg ctcccgacca gttcgggtct cgtcttggcg
     2881 ccgggttcga ccagccagca gcgcgcgatg cgcttctcgg cgatctgaaa ttccggcggt
     2941 tcctggacgt ggcaacgcgc gacggcggcg gtgcagcgcg gcgcgaagga gcagcccggt
     3001 ggcatagcgg ataaatcggg cggttgcccg tcgatcgcgg ccaggcgtga atgcgcatcg
     3061 ctcatgcgcg ggatcgagtt gagcaaagcc ttggtataag ggtgcgccgg cgcgttgaag
     3121 atgtcgcgca ccggtccgtg ctcgaccacc cgtccggcgt acatcaccgc gatctggtcg
     3181 cacatcctgg cgacaatgcc gagattatgc gtgatgaaaa tcatggccag gccatgccgt
     3241 cgctgcaggt cgctgagcag gctgagatac tgcgcctgga tcgtcggatc gagactggtg
     3301 gtcggttcgt cagcgatcag cagccgcggg ccgcaggaga tggcgatggc gccgacgata
     3361 cgctggcgca tgccgcccga catctcgtgc gggtactgcg tgacgcgctt ttcgggcgac
     3421 gggattcgca ccgatttcag cagctccacc gcccgcttcc acgcaccggc gcggctcgcg
     3481 ccttcatgca cgcggaccgg ttcgccgacc tgatcgccga tggtgaacaa tgggttaagc
     3541 gatgccatgg gatcctgcag gatcatcgcg atgcgcttgc cacgcaccgc acgcatctgc
     3601 tcgtcggttt tctccagcag gtcttcgccg tcgaacatca tcctgccgct gacgatgcgc
     3661 cctgccttcg gcaacagccg cagaatcgtc agtgcaagag tgcttttgcc ggagccggat
     3721 tcgccgacga tgcctagggt ttcgccggcg ttcagccgaa gcgtgacgtc gtccacggca
     3781 gggacaacgc gcgtgccctt ctgcgagacg tagtgggccg aaagattctc cagcgagaga
     3841 agcggtttcg cggtcatggc ttatcctcag agctggcgga gttgcggatc gagcttcacg
     3901 cgcagccagt cgcccagcag attggccgac aacaccatca gcatgatgca catccccggt
     3961 agtaccgtca gccaccagta gccgaccatc aggcccttct tgccgtccgc cagcatcagt
     4021 ccccaggcgg gtttcggcgg cggaacgccg acgccgagga aggacaacga ggcttcggta
     4081 acgacaacca cgcccagcat gagcgttgcc agcacgatgg cggagttgat gacgttgggc
     4141 aggatatggc gccacatgat cacgcgcttg gagcaaccgg cgacgatggc gaggcggacg
     4201 aattcacgtt ccttcagcga caacacttcg ccgcgtatga cgcgcgcgta gcgggtccag
     4261 taaacgccgc cgaggatgat gatgatgttg agctcactcg gaccgacgac ggcggcgagg
     4321 aagatcgcga aggtcagggc cggcagagcc agccaggtgt cggtgatgcg catgatcacc
     4381 tgatccaccc agccgcccag atacgccgag acgatgccca gaagggtgcc gatgctaccg
     4441 gcgaccagta ccgctgcggt gccgacgatc atcgacacgc gggcgccgaa aatcagccgc
     4501 gacagcacat cccggccgac atgatcggtg ccaagcaggt acttcatgct gccgccctgc
     4561 tgccaggccg gcgggcggaa acggtgcgcc agactgccca cttgcgggtc gtagggggcc
     4621 aggaagtcgg cgaagacggc gacaaacaga atgagagaca ggataagaat agggatcagc
     4681 ggaaatccat ccagccgcca gaaggcgaac gaccggcttg gggcggccgc gacggaaatg
     4741 cgatgtgtcg tgatgctcat cgtcagctct ccagacggat gcgcggatcg atcaaggcat
     4801 aaaggatgtc cacgatcagg ttgaccgcca cgatcatcgc gccgccgatc acgacgacac
     4861 cctgtacgat cgggaagtcg cggaacgtga tgccttcata aagcagcctg ccgatgccgg
     4921 gccaggcgaa caccgtttcc accaccaccg cgacgttgat catgactaca aggttgatgc
     4981 cggccagggt tacgaccggc agcagcgcgt tcttgaaagc gtgcttgccg atcaccagcc
     5041 gttccggcaa tcccttgagc cgcgccagct tgacatactc cgaaccgagc acgtcgagca
     5101 ttgaagatcg tgtcagccgc atgtgcgccg cggcgaaata ccatcccagc gcgaaggccg
     5161 gcatcagcag atgccagatg gtgccggaac ccgatgacgg cagccagcca aggtaaacgg
     5221 agaagaacag gatcatgacg agtgccaccc agaacgatgg caatgacagt cccagcaacg
     5281 cgaaaacctt gcccacgctg tcccacagcc gaccgacgtt gaccgcggcg agtaccccgc
     5341 tggggatgcc gatcagcagg gaaaagatca ttgccgcacc ggcaagcaac agggagttcg
     5401 gcagccgctg gaaataaagc tccagcaccg gggttctgta atagaacgag tcgcccaggt
     5461 cgccgcgtac aaagctcgcg atgaaatgcc agtactgcac ccaaagcggc tgatcgaggc
     5521 cgaactgcac gcggatcgct gccagatccg cggcgctggc gccaggctcc gccatcaggc
     5581 tcgccgggtc gcccgtcagg cggacgaaca gaaaaatcgt cagcgacagc aggaacagcg
     5641 acaagaacga gtagccgaca cgccgaacga tgtattgttg cattggtcgc ctcccagcgc
     5701 atcttcaggc tgatctcgca gccctgaaat gcttcagctt cttattcgac tgcacgattc
     5761 gcgtggttcg gaaattccag acaacacaat catgccctag gcccgggcct tcagcgtcag
     5821 gtcctcgtag ggtgcgggat agacaaaacc tttgataagc ccaaacccgg actggtccac
     5881 ccttggcccg acacccgaca ggaacgccaa ctgccaaatc ggcacatata tcgttttctc
     5941 gtgcgccagt tgttgcagcc tgtgcagaac cgaagtccgc ttcgcgcggt ccatctccga
     6001 tgcctgttcg gcgaacagcg cgtcgatatc cggatagttg ccatacgcgt aggtgccgcc
     6061 cttcacgaag aattcttccg tgcgggtcgc gcagttgccg aaggcgccgg aagctccctg
     6121 cacgatgtcc ttcagttttt tctcggaata agccttgatg aaggccgcac gctccagcgg
     6181 ccggagggtc gcacgcacgc ccagttcctg aaggttgttg atcgccactt cgccaatgtt
     6241 ggcatacgag gaatcacagt gataatcgcc ggcatcgaag cccgagggaa atccggcgtc
     6301 gctcagcagc ttcctggcct gggcaagatc atagaccggc gccggcggct gccagtaaaa
     6361 gtcgaaattc tcggggaacg cactgccagt gagatgggag tgtcccagcg tcagggcctc
     6421 gttcatcgtc ttacggtcga tcgccaggtt gacggcctgt cgcacgcggt aatcggacca
     6481 tggcgacttt gggttccatt gttgcgggaa atacagccaa tacggcgccg aaccaacggc
     6541 tggcttgatg gacaggcccg gagcgcgtga cagttcctcg gccagttcac cgcggataga
     6601 aagggcaata tcgacctcgc cgcgctgcaa cgcggccagc cgggttgcct cgtccgggat
     6661 caccttgaac accaggcgct tgatggaagg tttcttgcgc caatagccgt cgaacgcttc
     6721 caagaccagc tccacgcccg gtatgaaaga aaggaatttg tagggacccg cgccgactgg
     6781 tgccttcttg aacgcctcat cgccgatctt ttcgatgtat ttccttggga cgatccatcc
     6841 ggcccctgag gccgcggcat agaatgtcag gaaatcgggc catggcgctt tcaggcgaaa
     6901 gcgtacccgc gccggatcgg gagtctctat cgcggcaacg cggtcctgca tcagtcggtg
     6961 cgatgcaccg cggtagcgct cgaaggagaa tttcacgtcg tcggacgtca tcgtttcgcc
     7021 gttatggaat ttgactccct ggcgcagaac aaactcatgg ctcaggccat ccggactcat
     7081 ggtccaggac tccgcgaggc tcggtgccat cgaatttccc ggcatcgcct tcgcgaccgc
     7141 atcatgcatc gcgtacatca gcatgaatgg cgtaataagg ccggccgtct ccgccgggtc
     7201 gaaccaggtc ggcgccagcg agatgtgcgc gcctatcgta agggtcgcgc cgtcagcggc
     7261 cgcgggccaa gtcgtagcgc cgaacatcgt tgcagcggac agtccaagga caccgcgccg
     7321 cgtcgtggag ggattcatcc tggcgttcct tggctataca ccgggcttca agcgaaggtc
     7381 ctcataaggc gcgctatagg ggtgtccgcc gatcagccca agtgcagatt cctcgacccg
     7441 cgatccttgc ccgttgagga aggcaagctg ccagatcggc gcgtatatcg tgcgctcgtg
     7501 caaaagctgc tgcattcgat gcaggatggc ggttcgtttc gctcgatcga gctcgctcgc
     7561 ctgatcggcg aacaactggt caatatccgg gtaagtgcca tagacatagg cgccgtcctt
     7621 aacgacgaac gtctccatcc gggttgccgc gttgccaaag gcgccgctgc caccctgtac
     7681 aaggttccga tagcgctttt cggcataccc cttgttgaag gccgcgcgtt cgatcgggcg
     7741 cagcttcacg cgaagaccga cttcggcaag attgttgacc accgcttcgc caagattggc
     7801 gtaagacgaa tcgcagtaat actcgcccgc gtcgaaccca ttggggaaac ccgcttcagc
     7861 caataacttg cgcgcgccgg acggatcata tggtatcttt ggcggctgcc agtaaccatc
     7921 gtagtccttc ggcacaatgc tgtaggtgat tggtgaatac ccgagcgtga gggcttcgct
     7981 gatgccgaca cggtccaggg caagcgatac ggcttggcga acacgcacat cgtgccacgg
     8041 cgacttcgga tcccactggt ccgcgaagta aatccagaac ggcgagtgga tcacgatcgg
     8101 cttcagggtc agtccgggtg tgtcgcgcag ttcttcggcc agttccccac gaatggaata
     8161 ggccacatcg acctcgttgc gcttaagcgc cgcgaggcgg gtagtctcgt cgggaattac
     8221 tttccatacc agggtcttta cagccggggt cttgcgccaa tagcttgtga acgcttccat
     8281 cacgagttca acgccggggt tgaacgaagc aaatttgtag ggacctgcgc cgatcggcgc
     8341 cttcttgaag gcttcctcac caatgctctg cacatacttt ttcggaacga tccagcccgc
     8401 gccggtgacc ccggcgtagt aaataaggaa atccggccat ggctggcgca ggcgaaaaat
     8461 cacccgctgc ggatccgggg tttccaccgc tttgactttg ttcttcagga acgtcgcggc
     8521 gttgccacga taccgttcga aggagaattt gacgtcctcg gcggtaaccg gttcaccatt
     8581 gtgaaaggcc gctccggtgc gcagcttgaa ttcgtagctt agcccgtccg gggaaccggt
     8641 gcaggcctcc gcgaggcaag gctccgccgg gtttcccggc ataggtttca ccagggcgtc
     8701 atgcagcgca tacagcagca taaacggcgt gatgattccc gagacctcgg ccggatcgaa
     8761 ccatgttggc gccagcgaga tatgggcggc atagaccagc cggttgtttc gctctgctac
     8821 cgccggcatg gtttccgcgg cgagcacgcc gagcgctgtc acgccgagca aggcgcggcg
     8881 cgtgacgagg atgtcgttac tatcacgata caaatgcacg ataatcctcc actgttccgg
     8941 cggcttgcgc gccggttggg tgtctggatc gtctgatcgt catcgtatcg cacggcatga
     9001 tacgacttag ccggtcaatc gtttgaggcg gtacctccag ccagccacct cgggtcattc
     9061 acgaaaagca tccacggccg cactgggatt gctgcccgct gggatcagtc tcgtgatcgg
     9121 cgggcgtgcc ggctgcaggc gacgctttac gccttgcttc cctgatccac tggaaaacaa
     9181 cccctccgtg gttcaaggct ttgaacgtgc aaattgcgct gacccggcgt tgcggttgcc
     9241 ggcgaccgtg gaacctaccc caactcttct tatagcattg cagcgtatgt agggcagata
     9301 tgacgtgggc taataccctg gcggcggtaa agttttcgta acagcaaacg attccgccgg
     9361 ggaaatgcga accgaaaggt gatgcggcca ccgggccggg accgcatcac tttgcaattc
     9421 gcgaagtctg tttgttcagg ccgccttcgc caccgcccgc ttggatatca ctccgaccag
     9481 atgggcccgg tattcggcgc tggcgtggat gtctgcgttc atgtcggtcg ccggagtgac
     9541 gatcccgttg atcgcgtccg gggcgaaatt cgccgacagc gccttctcca tctcggtgtg
     9601 gcggaacacg ccgccctggc tggcgccggt gatcgccagg cgtacgccag agggaaattt
     9661 cgccacgaag acgccaacga tggcgtaccg tgaggctggg ttgcggaact tctcgtaggc
     9721 ggacttttcc gggatcggca gctcgatcga ggtgatgatc tcgccttgct ccagcgcggt
     9781 ggtgaacatg ccctggaaga agtcgtccgc ggcgatcgag cggcgatcgg tcttgatcgt
     9841 gccgccgagc gccagcaatg ccgacggata acaggcggcc gggtcgttgt tggccagcga
     9901 cccgcccatc gtgccgcgat ggcgcaccgc ctcgtctccg atcagcgacg cctgatgctg
     9961 caggccgggg atcttcgcct tgatctcagg attgctggcg atggcagcgt gcgtggtcat
    10021 cg
//
LOCUS       DASWQN010000604         4314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_717412, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000604 DASWQN010000000
VERSION     DASWQN010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717412"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VGC82_10300"
     CDS             complement(<1..309)
                     /locus_tag="VGC82_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0423702.1"
                     /translation="MTRARILLVEDEPLIRSLLAETLLEGGLEIVEAGSGEEAVEALG
                     QHDTFDLLLTDVHMPGGISGVDVARHARARDPDLPVVFATGRPESLNDFGTLGLREVC
                     L"
     gene            502..1650
                     /locus_tag="VGC82_10305"
     CDS             502..1650
                     /locus_tag="VGC82_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0423703.1"
                     /translation="MSDQNSTPAHEPRIAASGQGLLDRHVFYQAVKMTRMAMVLADPN
                     LPDCPLVYVNPAFHDLTGYTSEEAVGRNCRFLQGPETDPGVLERIRQALAAREVFNEE
                     IYNYRKGGAGFWNALHLSPVFDEDGKLLYYFGSQIDVSAQKEATRRQAQRIESIGALA
                     SGVAHEFNNLMTVVMGSVERAADRAADDRQSRHLTNADWAARRAGDLAAELLSLARRQ
                     SSAERIINLNELVQAFKNPLEQVAGPTMRLRFDLAPEAMLVRLDAGQFELVLLNLVRN
                     ATDAMAEGGEIVVATRMLQAAEASAALHSKAAVELSVTDTGAGMAPEVAKRATELFFT
                     TKSTGKGTGLGLFLALEFADHSGGKLTIDSEIGRGTTVRMNFPQATEY"
     gene            complement(1683..2804)
                     /locus_tag="VGC82_10310"
     CDS             complement(1683..2804)
                     /locus_tag="VGC82_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0423704.1"
                     /translation="MAALTVRRVSKRFGAVEVLKDVSLQIDSGEFTVLVGPSGCGKST
                     LLGIIAGLETASAGQIEIGGQPMNGVPAKDRNIAMVFQSYALYPAMTVRQNVTFGMEC
                     RGVPKALQNEAVARVARLLQIEPLLDRRPAQLSGGQRQRVAMGRALVRDPALFLFDEP
                     LSNLDAQLRVEMRAEIKQLHARIGTTCVYVTHDQVEAMTMATRIAVMHRGEVQQFDDP
                     DTIYNRPANLFVARFMGAPPMNTMPARLHGDGGEIHAVVGAGEDAIRLPLPLRGDDAR
                     LTPFRDMDVVLGIRPECIAESQRRFGLENMPPMMVDATVEMTEPTGAETLVILRLAGQ
                     RVIGRVAADTRLTLGRNERFSIDTRKLCVFDPSSERLIA"
     gene            complement(2815..3675)
                     /locus_tag="VGC82_10315"
     CDS             complement(2815..3675)
                     /locus_tag="VGC82_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0423705.1"
                     /translation="MNRRFRPSWGRWAIYGMLLALATFFLTPLVIVVLTSLRANEEMA
                     QRSLIGWPDAITLQNYTMAWSHFCIAERCLGVAPYIGNSLLMTVPATIISTLLGAVAG
                     FSIALWRFRGDTAIFALVTLGVFLPEQMRLIPWVIVLRDIGLSNTIAGLVVVHVVQGL
                     SFTTLFCRNYFVAIPQDLIKAARIDGAGFYRIFWRIVLPLSPPILIVCTIWQFTGIWN
                     EFLYGVTFLTGPDQPVTAAIISISATVTTSPQYGVQSAAVLLAALPTLIVYLFGGKYF
                     MRGLTAGAVR"
     gene            complement(3672..>4314)
                     /locus_tag="VGC82_10320"
     CDS             complement(3672..>4314)
                     /locus_tag="VGC82_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY0423706.1"
                     /translation="VLTSALGMLLAILLDQRVRGENIFRTIFLYPMAVSFVVTGTIWS
                     WLLNPGIGIQRLVQDWGWTSFRFDWLIDRDMAIYTVVIAGAWQAAGFAMALFLAGLRS
                     VDGEIIKAAQIDGAGAWRIYRRVVLPTIGPIVLAVMVILLQMAIKTFDLVRALTGGGP
                     GIATSMPTTVVYDFMFQRGQIGRGAAAAILMLLALVVVIVPYKAYQWWRERAR"
BASE COUNT          881 a         1410 c         1290 g          733 t
ORIGIN      
        1 cagacagacc tcccgcaaac ccaacgtgcc gaaatcgtta aggctttctg gtcgtcccgt
       61 cgcgaatacc acgggcaggt cgggatcccg cgcacgagca tgacgggcga catcgacacc
      121 actaatcccg cccggcatgt ggacatcggt cagcagcaaa tcgaacgtgt cgtgttgccc
      181 gagtgcttcc acggcttcct caccgctacc ggcctccacg atttcgaggc ccccttcaag
      241 cagggtctcg gccaaaagac tccggatcag gggttcatcc tcgaccagca ggattcgagc
      301 tctcgtcaac gaccacggac ctccttttcc gctcgcgaat aaaggccact ttggctgccc
      361 ataaagcgta cgctgacaac gctaaaccgc cgctgtcgtt caggggcagc agcaccgtga
      421 gcgccaacac gatcccgtga tttcgttgca ggtaacgccg aaccgcggaa ccagcctacc
      481 aaatcgatct tccaacgtag catgagcgat cagaactcca ctccagcgca cgagccgcgc
      541 atcgccgctt cgggacaagg tctcctggac cggcacgtgt tctaccaagc cgtgaaaatg
      601 acccgtatgg cgatggtgct ggcggacccc aacctgcccg actgtccgct cgtttacgtc
      661 aacccggcgt ttcacgacct gaccggttac accagcgagg aagcggttgg acgcaattgc
      721 cgcttcctgc agggacccga gacggatcca ggtgttctgg agcggatccg ccaggccctt
      781 gccgcacgcg aggtcttcaa cgaggagatc tacaactacc gcaagggcgg agcgggattc
      841 tggaatgccc tgcacctcag ccccgtcttc gacgaagatg gcaagttgct ctattacttc
      901 ggcagccaga tcgatgtcag tgcccagaaa gaagcgacgc ggcggcaggc gcagcggata
      961 gaaagcatcg gggcgttggc ctccggcgtc gctcatgaat tcaacaacct catgaccgtc
     1021 gtgatgggca gtgtcgagcg ggcagccgat cgggccgctg acgaccgcca gtcgcggcac
     1081 ttgaccaatg cggactgggc cgcccggcga gccggggatc tggccgccga gcttctttcc
     1141 ctggcgcggc ggcaatccag cgcggagcgg atcataaact tgaacgagct ggttcaggca
     1201 ttcaaaaacc cgctggagca ggtcgccggt cctactatgc gccttcgatt cgacctcgcg
     1261 cctgaagcaa tgctggttcg attggatgcg ggccaattcg aactcgtgct gctcaatttg
     1321 gtccgcaatg ccacggatgc catggccgag ggcggcgaga tcgtcgtcgc cacccgtatg
     1381 ctacaagcgg cggaggcctc cgcggccctc catagcaagg ccgcggtgga attatccgtc
     1441 accgacaccg gagccggcat ggcacccgag gtcgcgaagc gggcaaccga gttgttcttc
     1501 accaccaagt ccaccggcaa gggaaccggg ctcgggctgt tcctggcgct ggaattcgcg
     1561 gaccattccg gcggcaagct gacgatcgac agcgaaatcg gacggggcac gacagtgaga
     1621 atgaattttc cacaggcgac ggaatactga tcgccgaggc cccttcccgg aatcctccgg
     1681 catcacgcga tcagacgttc ggagcttgga tcgaacacgc acagcttgcg cgtgtcgatg
     1741 gaaaaccgtt cgttgcgccc cagggtcagt cgcgtatcgg ctgccacccg accgatgaca
     1801 cgttgcccgg cgagacgaag aatcaccagc gtttcggcgc cggtcggttc cgtcatctcg
     1861 acggtcgcat cgaccatcat cggcggcatg ttttccaggc caaagcgcct ttgcgattcg
     1921 gcgatacatt ccggacgaat acccagcaca acgtccatgt cgcgaaacgg cgtcagcctg
     1981 gcatcgtcgc cgcgcagcgg gagcggcaat cgaatcgcat cttcacccgc accgaccacc
     2041 gcgtggattt cgccgccgtc gccgtgcaac cgcgccggca tggtgttcat tggcggcgcc
     2101 cccatgaacc gggcgacgaa caggttcgcg ggacggttgt agatcgtgtc gggatcgtcg
     2161 aactgctgaa cctcgccgcg atgcattacc gcgatccggg tggccatcgt catcgcttcg
     2221 acctggtcat gggtcacata gacgcacgtc gtgccgatgc gggcgtggag ttgcttgatc
     2281 tcggcgcgca tctccacgcg aagctgcgca tccaggttgg acagcggctc atcgaacagg
     2341 aacagcgcgg gatcgcgcac cagcgcgcgt cccatcgcca cccgctggcg ctggccacct
     2401 gaaagctgtg ccggccggcg atcgagcagc ggctcgattt gcagcaggcg ggcgacccgg
     2461 gcaacagcct cattctggag cgccttcggc acaccgcggc attccattcc gaacgtgacg
     2521 ttctggcgca ccgtcatggc cggatacaag gcataggact ggaacaccat ggcgatgttg
     2581 cggtccttcg cggggacgcc gttcatcggt tgcccgccta tctcgatctg gccggcgctg
     2641 gcggtttcca atcctgcgat gatccccagc agcgttgact tgccgcagcc tgatgggccc
     2701 accaggacgg tgaactcacc gctgtcgatt tgcaggctta cgtccttcag cacttcgacc
     2761 gcgccaaaac gtttggaaac tcgccgcacc gtcaatgctg ccacgcccgt acccctaccg
     2821 taccgcgccg gcggtcaggc cgcgcatgaa atactttccg ccaaacagat agacgatcag
     2881 cgttggcagc gccgccagca acaccgcggc actttgcacg ccatattgcg gcgacgtcgt
     2941 taccgtggcg ctgatcgaga tgatggctgc cgtgaccggc tggtccggac cggtcaggaa
     3001 ggtgacgcca taaagaaact cgttccagat cccggtgaat tgccagatcg tgcaaacgat
     3061 caggatcggt ggcgaaagcg gcaggacgat gcgccagaag atacggtaga agccggcgcc
     3121 atcgatccgg gccgccttga tcaggtcctg cgggatcgcc acgaagtagt tgcggcaaaa
     3181 caacgtggta aagctcaggc cttgtacgac gtggacgacg accaatccgg cgatcgtgtt
     3241 cgacaggccg atgtcgcgca gtacgatcac ccacgggatc agcctcattt gctccggcag
     3301 gaagacgccg agcgtcacca gggcgaatat cgcggtatcg ccgcggaacc gccacagcgc
     3361 gatcgagaag ccggccaccg cgcccagcaa tgtggaaatg atggtcgccg gcaccgtcat
     3421 cagcaaagaa ttaccgatgt atggcgcgac gcccaagcag cgttcggcga tacaaaaatg
     3481 gctccaggcc atggtgtaat tctgcaacgt gatcgcgtcg ggccagccga tcaacgaccg
     3541 ctgcgccatt tcctcgtttg cccgcagcga tgtcagcacg acgatcacca gcggcgtcag
     3601 gaaaaacgtc gccagcgcca gcagcatccc atagattgcc cagcgtcccc atgagggtct
     3661 gaaccttcgg ttcatcgcgc ccgctcccgc caccactggt atgctttgta gggtacgatg
     3721 acgacgacca gtgcgagcag catcaggatc gccgctgccg cgccgcgccc gatctggcca
     3781 cgctggaaca tgaagtcgta aacgaccgtc gtgggcatgc ttgtcgcgat ccccggcccg
     3841 ccgccggtca acgcacgcac gaggtcgaac gtcttgatcg ccatttgcag caggatgacc
     3901 atcaccgcga gcacgatcgg cccgatcgtc ggcagcacca cacggcggta gatacgccag
     3961 gcaccggcgc cgtcgatctg cgcggccttg atgatctcgc catcgaccga acgaagtccg
     4021 gccaggaaca gcgccatcgc gaatccggcc gcctgccatg caccggcaat gacaaccgtg
     4081 tagatcgcca tatcgcggtc gatcagccag tcgaaccgga agctggtcca gccccagtcc
     4141 tgcaccagcc tttggatgcc gatgcccgga ttgagcagcc agctccagat cgtgccggtg
     4201 acgacgaagg acaccgccat cggatagaga aagatcgtgc ggaagatgtt ctccccgcgg
     4261 acgcgctggt cgagcaggat cgccagcaac atgcccagcg cactggtgag gacg
//
LOCUS       DASWQN010000605         1993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_59211, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000605 DASWQN010000000
VERSION     DASWQN010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59211"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..445)
                     /gene="hpxZ"
                     /locus_tag="VGC82_10325"
     CDS             complement(62..445)
                     /gene="hpxZ"
                     /locus_tag="VGC82_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalurate catabolism protein HpxZ"
                     /protein_id="HEY0423707.1"
                     /translation="MDVNLPEIKAEVEAAFAAYEQALMANDVDALQGHFWDSPYTIRY
                     GIAENLYGYNEIGAFRAARSPVGLARTTSRTVITTYGRDFATAATLFHRATAPGKVGR
                     QMQTWARTPDGWRVVAAHVSVIDAL"
     gene            complement(455..1846)
                     /locus_tag="VGC82_10330"
     CDS             complement(455..1846)
                     /locus_tag="VGC82_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtzE family amidohydrolase"
                     /protein_id="HEY0423708.1"
                     /translation="MTAAFATATDIAAAVASGRSTATGVMTACLDRIERLNPVLNAFT
                     AVTADRARLRAGALDAAIAAGVEPGPLAGVPFAVKNLIDVAGLPTLAGSKINRNHAPA
                     TADAPLIARLEAAGAILVGALNMGEYAYDFTGENVHEGPSRNPFDTARMSGGSSGGSG
                     SAVGGGLVPLALGSDTNGSIRVPSSFCGLFGLKPTYGRLTRSGSFPFVSSLDHLGPLA
                     RSTSDLAAAYDAMQGYDPTDPVCVDRPAEPVRGLFGSGIGGLRIASADGYFRTGASAE
                     ALAALDAIAAALGATESVTIPEVQRARSAAYVITTTEGAALHLNRLRTRPDDFDSDVR
                     DRLIAGALVPAAAVVQAQKFRRWFREAMLRLFQHVDAILAPATPCVAPLIGQKTFVLE
                     GVEMPLRPNIGIYTQPFSFIGLPVVAVPVPTPLGLPIGVQIITAPWREDIALRIAHAL
                     EASGAASAPVPNL"
BASE COUNT          363 a          665 c          668 g          297 t
ORIGIN      
        1 tcggccagga cccccagcag gtccaggatc cccagcgggt ccttgatcca aagcggggcg
       61 gtcacagggc gtcaatcacg ctgacatgcg cggcgacgac ccgccacccg tcgggcgtgc
      121 gcgcccaggt ctgcatctgc cgcccaacct tgccgggggc ggtggcgcga tggaacaagg
      181 tcgcggcagt cgcgaaatcc cggccatatg tcgtgatcac cgtccgcgac gtcgtccgcg
      241 ccagtcccac aggagaccgc gccgctcgga aggcaccaat ttcattgtag ccgtagaggt
      301 tctcggcgat gccgtagcgg atggtgtagg gactgtccca gaaatgcccc tgcagcgcat
      361 ccacatcgtt cgccatcagc gcttgttcat acgccgcgaa tgcagcttcg acctcagcct
      421 tgatctcggg caggttaacg tccaccgatc cgactcatag gtttggaaca ggcgcggacg
      481 cggcgccgga ggcttccaga gcatgagcaa ttcgcagcgc gatatcctct cgccacggtg
      541 cggtgatgat ctggacgccg atcggcaggc ccagcggcgt cggcacgggc accgcaacaa
      601 ccggcaggcc gatgaaagag aacggctgag tgtagatacc gatgttcgga cgcagcggca
      661 tttcgacgcc ttccaggacg aaggttttct ggccgatcaa gggagcaaca cagggcgtgg
      721 cgggcgccag gatcgcatcg acatgctgga acaggcgcag cattgcctcg cggaaccaac
      781 ggcggaattt ctgcgcctgc accacggcag cggcgggcac cagggcgccg gcgatcaacc
      841 tgtcgcgcac gtcggaatcg aagtcgtccg gccttgtgcg caggcggttc aggtgcaacg
      901 ccgcgccttc cgtggtggtg atgacatagg ccgcgctgcg ggcgcgttgc acctcgggga
      961 tcgtcacgct ctcggttgcg cccagtgcag cggcgatcgc atccagcgcc gccagggctt
     1021 cggccgatgc tccggtccgg aaatatccat cggccgaggc aatgcgcagg ccgccgatgc
     1081 cggaaccgaa caggccgcga accggttcgg ccggtcgatc aacgcaaacg ggatcggtgg
     1141 ggtcataacc ctgcatcgcg tcgtaggccg cagccaggtc agaggtggac cgcgccaagg
     1201 ggcccagatg atccaggctc gacacgaacg gaaagctgcc ggaccgggtc aagcggccgt
     1261 aagtcggctt cagtccgaac aatccacaaa aggacgacgg cacccggatt gacccgttgg
     1321 tgtccgaccc cagcgccagc ggaaccagtc cgccgccgac cgcgctgccg gagccaccgg
     1381 aggaccctcc ggacatgcgc gcggtgtcga acggattgcg cgacgggcct tcatgcacgt
     1441 tctcgccggt aaagtcgtag gcatactcgc ccatgttcag cgcaccgacc aggatcgctc
     1501 ccgccgcttc caggcgggcg atcaagggcg catcggcggt cgcgggcgca tggttgcggt
     1561 tgatcttcga cccggcgagc gtcggcaggc cggcgacatc gatcaggttc ttcactgcga
     1621 acggcacacc cgccagcggc cccggctcga cccccgccgc aatcgccgca tccaaagccc
     1681 cggcccgcaa ccgggctcgg tcggcggtga ccgcggtgaa ggcgttcagc accgggttga
     1741 gccgctcaat cctgtccagg cacgctgtca tgacgccggt ggcagtggaa cggccggatg
     1801 cgacggcggc ggcaatgtcc gtggcagtgg cgaaggcggc ggtcatgcgc gaaacaccgg
     1861 tgccggatcg agatggtccg gcaggtctgc ctgcagcacc gtcgcggcat ggtccaagga
     1921 gatcgccaga tgcatgcgca ccgccgggcg ccattgcgcc ttcatttcaa tgcccaaagc
     1981 cgcggtgccg gcg
//
LOCUS       DASWQN010000606         3673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1906538, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000606 DASWQN010000000
VERSION     DASWQN010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906538"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VGC82_10335"
     CDS             complement(<1..210)
                     /locus_tag="VGC82_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0423709.1"
                     /translation="MKATNRIGIREIRALQFGEIVWDGSVSGFGARRQSGPTVTYVLK
                     YRTVAGRQRFHRIGRHGSPWTPETAR"
     gene            524..1336
                     /locus_tag="VGC82_10340"
     CDS             524..1336
                     /locus_tag="VGC82_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0423710.1"
                     /translation="MSVLDGLRVVQIGPGLAAAVCGRLFADVGAAVSLIDPDRSTPLA
                     QHLNPTDGPSHPIEGAELIVCEGSPDTLRRTGRDPDSLRRINPSAAIVLISPFGQTGP
                     RAEEPATDLTLCFASGIARMLTGQVDDLDEPPMRPAGEQSAFIGGLAAACAGMHAALM
                     QNGATVDVSIHEALATLAITELARAGQTGKSWSRKRVADGNGATVCILPASDGYAAIS
                     PREDRQWAAWLQAMGSPGWGTEARFASKKDRVANWDELHALMSDWSRDKTKQ"
     gene            1448..2551
                     /locus_tag="VGC82_10345"
     CDS             1448..2551
                     /locus_tag="VGC82_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0423711.1"
                     /translation="MCGQTVRVPGSPFGIRTTTTVPNQPSPNADRDPGNTNRLPLSGI
                     RILDFSWVIAGPTATRYLAAMGAEVIKIEAPGRGDPGRTSELHTVLGQAKKGIVLDLK
                     NPNAIAIAKSLAANADVLVENFATGVMDRLGLGADAMRAENPGLIYVSASGLGRTGPE
                     ADAVAYGTLLQCYAGFAGLNRHSGMPPRVGMAWLDPMCGLMLAFITAASLWSRRSGTI
                     ARIDFSMIEAMLWTMAQPLLAAQLPSVKHAGARDAVYRCTGPDDWLATEPAQRPAPTE
                     RNAAGPLIRAGIAAAALARSSDLVACEHLRARGFWDPHGTGVLPGLPWRASFGQATGR
                     APDLDGDTDAVLRDVLGMSASDIASLRGTGALG"
     gene            complement(2586..3503)
                     /locus_tag="VGC82_10350"
     CDS             complement(2586..3503)
                     /locus_tag="VGC82_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0423712.1"
                     /translation="MPIEYEKREGVAYITLNNPAKANILDKETSDAISEAWIDLWEDR
                     DIRCAILTGKGDRHFCGGHNLTPRPDLTAEDREYLRTQRIYWPLAGTVHGQKTGVDGR
                     MGDHYPRVWKPVIAAINGWAAGAGLYMLLTSTDIRIASAEHARFKFSLLTQGWLGNGP
                     GASMLIKQLRYIDAMKILLTDEPFDAAEALRVGLVNEVVPHAQLMERAEKLARHIVTL
                     PPAAVRMMKEFVVRFGNLPDDQAWHVQNLINSLLIQTTMDGEEGRTAFNEKRPPNFTG
                     TLRKRGEPWPEPSEEDARRLDEAYRSGEF"
BASE COUNT          655 a         1254 c         1102 g          662 t
ORIGIN      
        1 acgcgccgtc tcgggcgtcc aaggcgagcc gtgccgacca atcctgtgga aacgctgccg
       61 ccccgccacc gtgcgatatt tgagcacgta ggtgaccgtt gggccggatt ggcgacgtgc
      121 tccgaatcca ctcacggacc cgtcccacac gatctcgccg aattgcagcg cccggatttc
      181 gcgtatccca attcggttgg tggccttcat ccatgacggt ctcgctcttc tccaacgacc
      241 gcgccagtag caaccgcgta gcaaccagca ccaccatgct cgagctaccc ggacgatggc
      301 gaacggcggc catgccattg acccagcaca attttctatc tcaaccgtcc tcgggccacg
      361 atctccgaag gcgaaggtcg cacgttcgaa tcgtgtcggg tccgccagtc atctaagttc
      421 cagggcttcc tgacggcccg cgcgaaacaa gccagtcaac gccaaggttt gtaaaccatg
      481 cgatctctgt gcatcgtttc ccggacggtg cagggaagct ggaatgtcgg tgctggatgg
      541 tcttcgcgtg gtgcagatcg ggccaggttt ggccgcggcg gtctgcggac ggcttttcgc
      601 cgacgttggt gcggcggtca gcctaattga tcctgaccgc tcgacgccgc tggcccaaca
      661 cctcaatccc accgacggcc ccagccaccc aatcgaaggc gcggaactga tcgtctgtga
      721 aggtagcccc gacaccttac gccgaaccgg ccgcgatccc gactcactcc gccggataaa
      781 tccttcggcg gcaatcgtgc tgatctctcc gttcggccaa accggaccgc gggccgagga
      841 accggccacc gatctcaccc tgtgcttcgc gagcggcatc gcccgcatgc tgacaggtca
      901 ggtggacgac cttgatgagc cgcccatgcg tccggcgggc gaacagtcag cgttcatcgg
      961 cggcctcgcc gccgcctgcg ccggcatgca tgccgcactg atgcaaaacg gcgcgaccgt
     1021 ggacgtctcc atccatgagg cgctggcgac cctggcgata acggagctgg ccagggccgg
     1081 gcagacaggc aaaagctggt ctcgtaagcg ggtggcagac ggcaatggcg ccacggtctg
     1141 catcctcccg gccagcgacg gttatgccgc catctcaccg cgggaagatc gccagtgggc
     1201 ggcgtggctg caagccatgg gatcaccagg ctggggcacc gaagcccgct tcgccagcaa
     1261 gaaagaccgt gtcgccaact gggatgaact gcacgccctg atgtcggact ggagccgcga
     1321 caaaaccaaa cagtagatcg ccgacaccgc ccaggccgca catgtcccaa gttttccgct
     1381 ctgcgaaccg gcggagcaac tggcatcccg gcagcttgaa caccgcggct tcttcaggtc
     1441 cgctcagatg tgcgggcaaa cggtgcgcgt gcccggatcg ccgttcggga tacggacaac
     1501 cacgactgtc ccaaaccagc cttcgccgaa cgccgaccgc gatcctggaa acacaaatcg
     1561 cctgccattg tctggtatcc gcatcctcga cttcagttgg gtgatcgccg ggccaaccgc
     1621 cacgcgctat ctcgcggcga tgggggcgga agtcatcaag atcgaggccc ccggacgcgg
     1681 cgatcctggc cgcacatccg aacttcacac cgtccttggc caggcgaaga agggcatcgt
     1741 cctggacctg aaaaatccca acgccatcgc cattgccaaa tctctggctg ccaacgccga
     1801 cgtgctggtg gaaaacttcg ccaccggcgt gatggaccgc cttggcctgg gcgccgacgc
     1861 catgcgcgcg gaaaaccccg gattgatata cgtctccgcc tccggtctcg gccggacagg
     1921 cccggaggcg gatgcggtcg cgtatggcac gctgctccaa tgttacgcag ggtttgccgg
     1981 gctgaaccgc cactccggca tgccgccccg cgttgggatg gcttggctcg atccaatgtg
     2041 cgggctgatg cttgcgttca tcaccgccgc cagcctctgg tcccgtcgct ccggcaccat
     2101 tgctcgaatc gacttctcga tgatcgaggc catgctttgg acaatggccc aaccgctgct
     2161 ggccgcccag ctgccgtccg tgaaacacgc gggcgccagg gatgcggtct atcgctgcac
     2221 cggccccgat gactggcttg ccaccgaacc agcgcaaagg ccggccccta ccgaacgcaa
     2281 tgccgcgggc cctttgatcc gagccgggat cgcggccgcg gcactggcca gatcgtcgga
     2341 cctcgttgcc tgcgagcatc tccgggcgcg tgggttctgg gatccccatg gcacgggtgt
     2401 gctgccaggc ctgccgtggc gcgcgtcgtt cgggcaagcc accggccgcg cacccgattt
     2461 ggacggggat accgacgcgg tattgcgcga tgtgctgggg atgtcggcgt ctgacatcgc
     2521 ctcactgcga ggcaccggcg cattgggctg aggcggccgg atacaaaaac cggcgaccga
     2581 accagttaaa attcgccgct gcggtaagcc tcatccaatc gccgtgcgtc ctcctcggac
     2641 ggttccggcc acggctcgcc gcgcttgcgc aacgtgccgg tgaaattcgg tggccgcttc
     2701 tcgttaaacg ccgtccgccc ttcctcaccg tccattgtcg tctggatcag caacgagttg
     2761 atcaggttct gcacatgcca ggcctggtcg tccggcaagt tgccaaaccg gaccacgaac
     2821 tccttcatca tccgcaccgc tgccggcggc agagtaacga tatgccgcgc caacttctcc
     2881 gcccgctcca taagctgcgc gtgcggcacg acctcgttca ccagaccgac ccgcagtgcc
     2941 tccgccgcat cgaagggttc gtcggtcaac aggattttca ttgcatcgat gtagcgaagc
     3001 tgcttgatca gcatgctcgc acccggaccg ttcccgagcc acccttgggt caacagcgag
     3061 aacttgaacc ttgcatgctc cgcactggcg atccggatgt cggtcgaggt cagcagcatg
     3121 taaagtcccg cgccggccgc ccagccattg atcgcggcga tgaccggctt ccagaccctg
     3181 gggtaatggt cccccatgcg tccatccacc ccggtcttct gcccgtgcac cgtccccgcg
     3241 agcggccagt agatgcgctg ggtgcggaga tattcgcgat cctccgccgt caggtccggc
     3301 cgaggtgtca ggttgtgccc gccacagaaa tgccggtcgc ctttgcccgt aagaatggcg
     3361 caccttatgt cgcggtcttc ccagagatcg atccaggctt ccgagatcgc atcggacgtc
     3421 tccttgtcca ggatattggc cttcgccggg ttgttcaggg tgatgtaggc caccccttcc
     3481 cgcttttcat actcgatggg catcgttggg tttctcccga aaggcatcat tcccggcaag
     3541 tccggctgga ccggattcca ccgcgcggat gcccaggttg caagcccgcc ggacggaccg
     3601 tcgatggcga gctgaccgtg caagcagtgc tgagcgtcga tacttgacgc ttggcgacgt
     3661 taaggcaaag ctt
//
LOCUS       DASWQN010000607         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1113843, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000607 DASWQN010000000
VERSION     DASWQN010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113843"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VGC82_10355"
     CDS             <1..960
                     /locus_tag="VGC82_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY0423713.1"
                     /translation="KIAMFLMLLGLGGLNFLLVERLRANPATSVGRLRRFAEVEFGIG
                     IAIFFAAASLTSVPPAVDLAQDRVTWPEIQERNTPMWPRMTSPDHDDLALPALQAKLD
                     AEAAAHRAAPQAAFVPGGGELAPRNADDIAWSEYNHHWAGLIVIAVGLLALANRAGLR
                     IARHWPLAFLGLAVFLFFRSDPETWPMGNIGFLESFRDVEVLQHRFFVLLILVFTWFE
                     WRVRATNWTNRYAAYVFPLLCAVGGTMLLTHSHAISNVKDQLLIELTHTPLALAGIAA
                     GWGRWLEIRLNPRENPLAWHIAGWVWPVCILFCGLLLLGYREA"
     gene            complement(975..>1936)
                     /locus_tag="VGC82_10360"
     CDS             complement(975..>1936)
                     /locus_tag="VGC82_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0423714.1"
                     /translation="KLSDHFDGQHFFNPNVAPSSRGLVPLFRWRMSGDRAAWPAAVSD
                     PKFPPPPGSVSPGSAAITFINHASFLIRLPDAVILTDPIFSQRCSPVSWAGPKRARPP
                     GVLLDALPRPDLVLLSHNHYDHMDLPSLREIQRRHAPAFVTTLGNAAALAKIGITATE
                     LDWWQTVALGNLRISATPARHFSARTPFDRNRTLWAGFMIASGSGRILFAGDSAAGSH
                     WQDIRTRLGPPDVALLPIGAYEPRWFMAAAHMNPEEAVQAHTALGARQSIGMHYGTFQ
                     LTDEAIDAPLIALAEAKRAANLAPDQFTTLGFGETRVYPSGET"
BASE COUNT          318 a          583 c          695 g          340 t
ORIGIN      
        1 aagatcgcca tgttcctgat gctgctcggg ttgggcgggc tgaacttcct gctcgtggag
       61 cggttgcggg ccaatcccgc gacctcggtc ggacgcctgc ggcggttcgc ggaggtggag
      121 ttcgggattg gcatcgcgat cttcttcgcg gcagcgtccc tgacctcggt gccgcccgcg
      181 gtggacctcg ctcaggaccg ggtgacgtgg ccggaaatcc aggagcgcaa cacaccgatg
      241 tggccgcgga tgaccagccc tgaccacgac gacctggcgc tgccggcctt gcaggcaaag
      301 ctggacgccg aggcagcggc gcatcgggca gcgccccagg cggccttcgt gccgggcggc
      361 ggggaactgg ccccgcgcaa cgccgacgac atcgcctggt cggaatacaa ccatcactgg
      421 gccgggttga tcgtgatcgc ggtcgggctg ctggcgctgg cgaaccgggc ggggctgcgc
      481 atcgcccggc attggccgct ggcgttcctg ggcctggcgg tgttcctgtt cttccgatcc
      541 gaccctgaga catggccgat gggaaatatc ggattcctgg aaagcttccg cgacgtggag
      601 gtgctgcagc accgcttctt cgtgctgctg atcctggtgt tcacgtggtt cgagtggcgg
      661 gtgagggcaa cgaactggac caaccggtac gcggcctatg tgtttccgct gctatgcgcg
      721 gtgggtggga cgatgctgct gacccacagc catgccatct ccaacgtgaa ggaccagttg
      781 ctgatcgagc ttacgcacac gccgctggca ctggccggca tcgccgccgg ttggggcagg
      841 tggttggaga tccggctgaa cccgcgcgaa aacccgctcg cgtggcacat cgccggttgg
      901 gtatggccgg tgtgcatcct gttctgtgga ctgctgctgc tagggtaccg ggaggcctga
      961 cgtctatccg tttgtcacgt ttcgccggac ggataaacac gggtttctcc aaacccgagc
     1021 gtggtgaact gatcaggcgc caggttcgcg gctcgctttg cttctgccag agcgatcagc
     1081 ggcgcgtcga tcgcttcatc ggtgagctgg aacgtgccgt agtgcattcc gatcgactgc
     1141 cgcgcgccca gcgctgtgtg ggcctgcacg gcttcctccg ggttcatgtg cgcggccgcc
     1201 atgaaccagc gcggctcgta ggccccgatg ggcagaagtg cgacatcggg aggaccaagg
     1261 cgggtgcgga tatcctgcca gtgtgacccg gcggccgaat cgccggcgaa caggattcga
     1321 ccgctgccac tggcgatcat gaagccggcc cagagggtgc ggttgcggtc gaatggcgtg
     1381 cgggccgaga aatgacgggc gggcgtcgcg gagattcgca ggttccccag cgcgaccgtt
     1441 tgccaccagt ccagttccgt cgcggtgatg ccgatcttgg ccaaggcagc ggcgttgccc
     1501 agcgtggtga caaaggccgg ggcgtgacgg cgctggatct cccggagact gggcaggtcc
     1561 atatggtcgt agtgattgtg tgacagcagc accagatcgg ggcgtggcaa agcatcaagc
     1621 agcacaccgg gcggacgcgc ccgtttcggt ccggcccacg acaccggcga gcagcgttgg
     1681 ctgaagatcg ggtctgtcag aatcaccgcg tccggcaagc ggatcaggaa gctggcgtgg
     1741 ttgataaagg tgatcgcggc cgaaccgggc gagaccgaac caggaggcgg cggaaacttg
     1801 ggatcggaca ccgcagccgg ccacgcggcg cggtcaccgc tcatccgcca tcgaaagagt
     1861 ggcaccaagc cgcgggagga tggagccacg ttcgggttga agaagtgctg gccgtcgaaa
     1921 tggtccgaga gcttgt
//
LOCUS       DASWQN010000608         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_982322, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000608 DASWQN010000000
VERSION     DASWQN010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982322"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..>2814)
                     /locus_tag="VGC82_10365"
     CDS             complement(216..>2814)
                     /locus_tag="VGC82_10365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0423715.1"
                     /translation="MMAAKGIIRPIEQLGRLATHDDWVDLPSPVTTGLPETDLVACAL
                     VAAAADRRAGAAERQAGAQRLAESEQRFRSLFEKSSSGTILLDPETTRIIDCNEVAAS
                     IVGYSVEEFRTCSITDFALQTTPERIRELCQSVAEGASLRYETRIVGRQGPRDVLVGV
                     APVQVSGRTLVLLNQIDITDRRRAEAGLRINEERLELAREGANLGIWDWNIANNTLSW
                     SDHQWHLHGLDPQSDGPSPEVWRQTVHPDDLLRVQEELVAALKVPGRPYATEYSVILP
                     DGSFRRLLGRGQSIRGADGRTVRMVGINMDVTARYEAEMARDRLIWMLETERSRLSEI
                     IEALPIGVGIVDAQGRALLGNAVMKRLNGPVLNSMAAAPRGEWIGEDPQGNRVAPEDY
                     PVRRALRLGETTLPGRDFLFRDEDGSETWFRVGSIPLRWDNGQVQEALGIFLDIDAEK
                     RLLDVQQQITARLEQRVSEEVTAREAAQKRAAHAERMHALGQIAGGIAHDFNNVLQAV
                     LGGATLIESRPKNVERVVRHARMVLDAATRGAAITSRLLAFSRRGDLRAEPVDSAALL
                     ADMVEVLTHTLGGSVLCVVEALPGLPPLFADRGQLETVLVNLATNARDAMPSGGTLTL
                     AADSEVVSAGLPHPSGLGVGEYVRITVSDTGTGMDRAVLARVTEPFFTTKEPGKGTGL
                     GLAMAKGFVEQSGGSLAIDSTVGFGTRISLWLPTATGTVGATGDFPSEVRAGNERAYV
                     LLVDDDAIVREILTLSLEDAGYAVHPTDSGATALSWLAADEPVDIIVTDLTMPGMDGL
                     ALIRAAQKQRPNLPAVLVTGYAGDGAALAVGGAISGQFTLLRKPVSSTQLVDRINALL
                     ASRKQESKA"
BASE COUNT          501 a          949 c          838 g          526 t
ORIGIN      
        1 ggccacagac cctagtatta tagcgttcag acaaagtcgt aggtggccgc gccaagcacg
       61 gccatgacac gatcatcgtc cgctgcgcca gttccaagca ctgcgcaagc attgctttgg
      121 cgctcgtgga cgctcgtcga ccatgacggg atcacaaatc gcggctgaaa cggtcattcg
      181 gatcagacct aatcctcccg cctggggaaa taaactcaag ccttgctttc ctgctttcgg
      241 gatgccagca aagcgtttat ccggtccaca agttgcgtgc tcgaaaccgg tttgcgcagc
      301 agggtaaact ggccgctgat agcgccaccg acggccagcg cggcgccgtc gccggcatat
      361 ccggtcacca gtacggctgg caggttcggc cgttgcttct gcgccgcgcg gattaacgcc
      421 agcccgtcca tccccggcat cgtcaggtcg gtgacaatga tgtccacggg ttcgtccgcc
      481 gccagccatg acagcgccgt cgcgccgctg tcggttggat gcaccgcgta tcccgcgtcc
      541 tctagcgaaa gcgtcaggat ctcgcggacg atcgcatcgt cgtccaccag cagcacatac
      601 gctcgttcgt ttccggcgcg aacttccgat gggaaatcgc cagtggctcc caccgtaccc
      661 gtcgcagtgg gcagccagag gctgatgcgg gtgccgaacc cgacggtgct gtcgatcgcc
      721 aggctgccac cgctttgttc gacgaagccc ttggccatcg ccagacccaa tccggtgccc
      781 ttgcctggct ccttggtcgt gaagaagggt tcggtcaccc gggcgagcac ggcacgatcc
      841 ataccggttc cggtatcgct caccgtaatc cggacatatt caccgacccc caggccggac
      901 ggatgcggca acccggccga gacgacctcg gaatcggctg ccaaggtcag cgtgccgccg
      961 gacggcattg cgtcgcgtgc attggtcgcc agattgacca gcacagtttc cagctgcccc
     1021 cggtcggcga acagcggcgg cagtccgggc aatgcctcca ccacgcagag caccgaaccg
     1081 cccagcgtgt gcgtgagcac ttctaccatg tcggctagca atgcggcgga gtcgaccggt
     1141 tccgcgcgca ggtcgccacg gcgcgagaag gccagcagcc ggctggtgat cgccgccccg
     1201 cgggtggctg catccagcac catgcgggcg tgacgcacca cccgttcgac gtttttcggc
     1261 cggctttcga tcagtgtcgc gccacccagc actgcctgaa gcacgttgtt gaagtcatgc
     1321 gcgatgccgc cagctatctg gccaagcgca tgcattcgtt cggcatgagc ggcgcgcttt
     1381 tgcgcggctt cgcgcgccgt cacttcctcg gacacccgct gttccaggcg ggcggtgatt
     1441 tgctgctgga catccagcag gcgcttttcg gcatcgatgt cgagaaagat accgagtgcc
     1501 tcctggacct ggccattgtc ccaacgcaac ggaatgcttc ccacccggaa ccaggtttcg
     1561 cttccgtcct cgtcgcgaaa aaggaagtcc cgccccggca gggttgtctc acccaggcgc
     1621 agagcgcggc gaaccgggta gtcctcgggc gccacgcgat ttccctgcgg atcttcaccg
     1681 atccactcgc cgcgcggcgc cgctgccatg gagttaagga ccggcccatt cagccgtttc
     1741 atcaccgcgt tacctagaag tgcccgccct tgcgcatcga cgatcccgac accgatcggc
     1801 aacgcctcga taatctccga cagccggctg cgttcggttt ccagcatcca gatcagccgg
     1861 tcgcgggcca tttccgcttc atagcgagcc gtgacatcca tattgatgcc gaccatgcgc
     1921 accgtgcggc cgtccgcccc gcggatcgac tgtccccggc cgagcagccg gcggaacgac
     1981 ccgtccggca ggatcacact gtattcggtc gcatacggac gacctgggac cttcagcgcg
     2041 gcaaccagtt cttcctgcac gcgaagcagg tcgtcagggt gcaccgtttg acgccagact
     2101 tccgggcttg ggccatcaga ttggggatcc aagccatgca agtgccattg atggtcgctc
     2161 cagctgagtg tgttgttggc gatattccag tcccagatcc caagattcgc accttcccga
     2221 gcgagttcca gccgctcttc attgatgcgt aggccggcct ccgcgcggcg ccggtcggta
     2281 atgtcgatct ggttgagcag caccagggtc cggccggaaa cctggaccgg cgcaacccca
     2341 accagaacat cgcgcggacc ttgccggccg acgatgcgcg tctcgtatcg caggctggcg
     2401 ccttcggcga cggactggca cagctcccga attcgctccg gagttgtttg cagagcaaag
     2461 tcggtgatcg agcaggtgcg gaattcctcg acggaatagc cgacgatcga tgccgccacc
     2521 tcgttgcaat cgatgatgcg cgtggtttcc gggtcgagta aaatcgttcc gctagaagat
     2581 ttttcgaaca aagaacgaaa acgttgttca ctttccgcca gtcgctgggc tcccgcctga
     2641 cgttccgcgg cccccgcccg acggtctgcg gcggcagcga ccagcgcaca tgcgaccaag
     2701 tcggtttccg gcagtcctgt tgtgacgggg ctgggcagat cgacccaatc atcgtgcgtg
     2761 gccaaccggc cgagttgctc gatcggacgt ataatgccct tggccgccat catc
//
LOCUS       DASWQN010000609         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_323172, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000609 DASWQN010000000
VERSION     DASWQN010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323172"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VGC82_10370"
     CDS             <1..875
                     /locus_tag="VGC82_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423716.1"
                     /translation="STVMIPAFGWRSMFVLGGIGALVVWYIRKALPESPRWLESVGRT
                     DEAEALLTKIEASVSGPLAAVGPSASAPSRALATLFAPPLASRMVLGCIALIVINTLI
                     YGFITWLPSFFVKQGLSIATSFNFTLIMSLGAPVGAAIGAMTADRWGRKPTLIGASVV
                     AIVFGAIYPFVTDPTLLPLAGIALTVPIYVLVALLFGIYIPELFPTEVRLRASGICNT
                     FGRAATIVTPFLVVKLFTDFGVVGVLSLMIGLLFVLILTVAALGIEPRRRSLEDVTSL
                     SDATGTATTSATAR"
     gene            943..3135
                     /locus_tag="VGC82_10375"
     CDS             943..3135
                     /locus_tag="VGC82_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY0423717.1"
                     /translation="MARYTADERFEGAAFAAFVRSPHAHARIVRLDTAEAAAMSGVVR
                     VLTAQDCAGLANFPVMDRIGKGLAIPFRPVLASDVVRHPWEAVACVVAETPAGALDAA
                     EAVAVDYDALPAAVGLIDGAPLVHPGAPGNVAMRHEAGDAAAVSAAIAAAALVIETEI
                     ELPRLAPVTLEPRASAARWDAGNGVYIIRAPHQGVNEMRRDFAAVFGLPADRFHVMDG
                     EVGGGFGPRNIAYPEYAAVMLAARLTERPVVWHGTRSESFLTDIQGRGVRVRGRLAVD
                     AAGRFTALSMEYDADLGAYISPVGVFANVNNPLQSLTGCYAIPAAHAVFRMMHTNSVP
                     TGPYRGAGRPEMALLVERLVDIAARRLGLDPFLLRERNIIPADAFPWTIPSGARYDSD
                     DFSRLMRTAREASDWDGFKTRSAPTGFRSGRGMALFIEVSGGGGAPDEAALTLSAVDG
                     QAALRIETVTASTGQSHSRTFALVAGPRLGLPEASVELVASSPATALSGAGSYASRST
                     IAAGSAVAAAAEALSLRIRSMAALRANCAPEDLHLADECVCRADGSVVCRIVDLLGEP
                     MLETGRVMPVQAFASGCHVAEVAVDEATGATVLTRYLAVDDAGVTIDPQAAEAQIQGG
                     IAQGVGEVLGEEAVIDVASGQPYAASLMDYMLPRANDFPNYRVMECNTPSPSNPLGVK
                     GIGEAGTTGALCAVTSAVADALGGRELPAMPFTQERLWRVLQDYDTSS"
     gene            complement(3213..>3491)
                     /locus_tag="VGC82_10380"
     CDS             complement(3213..>3491)
                     /locus_tag="VGC82_10380"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0423718.1"
                     /translation="VRTHPDNHRKSIYINPIRIEGIVGLDHREALKLLDDLLEHATQQ
                     AFQYRHEWRPGDLVMWDNRCLLHKANGDYDMDQTRYLYRVMLQGDIPR"
BASE COUNT          548 a         1017 c         1227 g          699 t
ORIGIN      
        1 ccagcactgt catgatcccg gcgttcggct ggcgcagcat gttcgtgctg ggcggcatcg
       61 gtgcgctggt ggtttggtac atccgcaaag ccctgcccga aagcccgcgc tggcttgaat
      121 cggttggccg gaccgatgaa gccgaggctt tgctgacgaa gatcgaggcg tcggtttccg
      181 gacccttggc cgccgtcggg ccatccgcgt ccgccccttc tcgcgcactg gcaacgctgt
      241 tcgcgccacc gctggcgtca cgcatggtgc tgggctgcat cgcgttgatc gtcatcaata
      301 cattgatcta tgggttcatc acctggctgc cttcgttctt cgtcaagcag ggactttcga
      361 tcgcgacatc gttcaacttc accctgatca tgtcgttggg cgctcccgtc ggggcggcga
      421 taggcgcaat gacggcggat cgctggggac gcaaaccgac attgatcggc gcttccgtcg
      481 tggcaatcgt attcggtgcg atctacccat tcgttaccga tcccacgctg ctgccgttgg
      541 cgggaatcgc actgacagtt ccaatttatg tgctggtcgc cttgttgttc ggtatctaca
      601 ttcccgaact gttcccgaca gaggtgcgcc tgcgcgcgtc gggcatctgc aacacattcg
      661 gcagggccgc gacgatcgtc acgcctttcc tggtggtcaa actgtttacc gatttcggcg
      721 ttgtgggtgt gctgtcgctg atgatcggcc tgttgttcgt gctgatcctg accgtcgcgg
      781 cgctggggat cgagccccgg cgccggagtc ttgaggacgt aaccagcttg tcggacgcta
      841 cggggacagc aacgacgtcg gcaaccgcgc gataaaggtt cgggtatgga caggggtgga
      901 caaccgcagg cagaaaccga gcggtcgagg aagctgcgcg gtttggcgcg ctatacggcg
      961 gatgaacggt tcgaaggagc ggctttcgcg gccttcgttc gcagcccgca cgcccacgcg
     1021 cggattgttc gcctcgacac ggccgaggcg gcagcgatgt ccggcgtggt acgtgtgctg
     1081 accgcgcaag actgcgcggg actagcgaat tttccggtga tggaccggat tggcaaaggc
     1141 cttgcgatcc ccttccgtcc ggttctggcg tccgatgtgg tgcgccaccc ctgggaagcg
     1201 gtggcctgcg tggtcgccga aaccccggct ggggcgctgg atgcggccga ggctgtcgcg
     1261 gtggattatg acgcgctgcc ggcagcggtc ggcctgatcg atggcgctcc tctggtgcat
     1321 cccggtgcgc ccggcaatgt agccatgcgc cacgaggccg gggacgccgc ggcggtcagc
     1381 gcggcgatag cggcagccgc attggtgatc gaaaccgaga tcgagctgcc tcgcctggcg
     1441 ccggtaacgc tggagccgag ggcctcggcg gctcgttggg acgccggcaa cggggtttac
     1501 atcattcgcg cgccacatca gggagtgaac gagatgcggc gcgatttcgc cgcggtgttc
     1561 ggcctgccgg cggatcggtt tcatgtaatg gacggcgagg tcggcggcgg cttcggaccg
     1621 cggaacattg cgtatccgga gtatgcggcc gtgatgcttg ccgcgaggct gacggagcgg
     1681 ccggtggtct ggcatggcac gcggagcgag tcgtttctga ccgatatcca aggtcgcggc
     1741 gtcagggtac gcggacggct ggcagtggac gcggcggggc ggttcaccgc gttgtcaatg
     1801 gaatatgacg ccgatctggg cgcgtatatc tcgccggtcg gcgtgttcgc caacgtcaac
     1861 aatccgttgc agtcgctgac cggatgttat gccattccgg cggcgcacgc ggtgttccgg
     1921 atgatgcata caaattcggt accgaccggg ccgtatcgtg gggcagggcg cccggagatg
     1981 gcgctgctgg tcgaacggct ggtggatatc gccgctcgcc ggctggggtt ggatccgttc
     2041 ctgttaagag aacgcaatat cattcccgct gatgcgttcc cttggaccat ccccagcgga
     2101 gcgcgatatg acagcgacga tttctcgcgg ctgatgcgga ccgcgcggga ggcttcggac
     2161 tgggacggtt tcaaaacgag atcggcgccg acaggatttc gctccggccg cgggatggcg
     2221 ctgttcatcg aggtctcggg cggcggcggc gcaccggatg aggcggcgct gacgctgtca
     2281 gccgtcgatg ggcaggcggc actgcggatc gaaacggtaa cggccagcac aggccagtcc
     2341 cattcgcgta ccttcgcatt ggtggcgggt ccacggttgg ggctaccgga ggcttcggtc
     2401 gaactcgtgg catccagtcc tgcaacggct ttgtcggggg ccggatcgta tgcctcccgc
     2461 agcacgattg cggctggcag tgcggtcgcg gcggctgcgg aggctctatc gttgcggatt
     2521 cgctcgatgg cggcgctgcg ggcgaactgc gcgccggagg atctgcatct ggcggatgaa
     2581 tgcgtctgtc gcgctgatgg cagcgtcgta tgccggatcg tggatctgct cggagagccg
     2641 atgctggaaa cgggccgggt gatgcccgtc caggcctttg cctccggttg tcatgtggcc
     2701 gaggtcgcgg tggatgaggc gaccggggca acggtgctga cacggtacct cgcggtggac
     2761 gacgccgggg taacgatcga tccgcaggcg gcggaggcgc agatccaagg tggaattgcc
     2821 cagggcgtcg gtgaggtgct gggtgaggag gcggttatcg atgtggcgtc cggccaaccc
     2881 tacgcggcga gcctcatgga ttacatgctg ccgcgtgcca atgatttccc gaattaccgg
     2941 gtgatggaat gcaacacgcc gtcgccgtcc aacccactgg gtgtgaaggg catcggcgag
     3001 gccggaacga ccggtgcatt gtgcgcggtc accagcgcgg tggcggatgc gctgggcggg
     3061 cgcgaactgc cggcgatgcc attcacgcag gagcggctgt ggcgggtgct tcaggattat
     3121 gacacgtcgt catagtacct cgaaggctcg tcggtgttgg ttcccgcaag cgtcgcctct
     3181 tccggagaaa aggcggggcg cattttgctt ggttaccgcg ggatgtcgcc ttgaagcatt
     3241 acgcgataca aataccttgt ctggtccatg tcgtagtcgc cgttcgcctt gtgcagcagg
     3301 cagcgattat cccacatcac gagatcgccc ggccgccact cgtgccgata ctgaaacgcc
     3361 tgctgtgtcg cgtgttccag caaatcgtcc agcagcttca gtgcctctcg atgatccagg
     3421 ccgacgatcc cctcgatgcg gatcggattg atgtaaatcg acttgcggtg attgtccgga
     3481 tgggtgcgca c
//
LOCUS       DASWQN010000610         4896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_982379, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000610 DASWQN010000000
VERSION     DASWQN010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982379"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1044)
                     /gene="pdxA"
                     /locus_tag="VGC82_10385"
     CDS             complement(40..1044)
                     /gene="pdxA"
                     /locus_tag="VGC82_10385"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY0423719.1"
                     /translation="MAKPLALTLGEPAGIGPDITIKAWLRRNELKLPPFYLLGDRAFL
                     AGRAKALGLDVKLAGVSAEEARDAFADALPVVATGIAATANPGRPDDRSAEAALASIR
                     QAVGDVVAGRAGAVVTNPVAKNVLYRAGFRHPGHTEFLAELAAGGGRAPQPVMMLWSP
                     ALAVVPATIHLPLRDAISQLSSDLIVTTARIVVGDMKARFGIANPRLAVSGLNPHAGE
                     DGSLGTEDQAVVAPAVEILRGEGIDVRGPLPADTMFHDAARKTYDCAICMYHDQALIP
                     VKTLAFDDAVNVTLGLPFIRTSPDHGTAFDIAGTGRANPSSLIAALRLAARIAATTQA
                     "
     gene            complement(1048..1920)
                     /locus_tag="VGC82_10390"
     CDS             complement(1048..1920)
                     /locus_tag="VGC82_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423720.1"
                     /translation="MTCTASPLRAQTVAVMVNGEPITNYDIEQRSKLNFLTTHKAADR
                     KQVINELIDEKVKIKEGKKFGVDPGVSDIDQSFAAMSSRMRITPDQLTKSLESQGIRA
                     DTLKARIKAEMVWTSLVRGRYKESLQVGEKDVAAAVQGSGDEKQEAEAFEYKMQPIVL
                     IVPRGSAPAAIEARQKEAEVLRNRVQTCDDANALFKSMQNAAIREAVIKTSADIPPVL
                     RDLLDKTPIGHLTPPEVTKQGVEMVALCGRKPTTVDSPKKKAVRDKMFSDKYEAKSKA
                     YLQEVRKAAMIEYR"
     gene            complement(2074..4578)
                     /locus_tag="VGC82_10395"
     CDS             complement(2074..4578)
                     /locus_tag="VGC82_10395"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEY0423721.1"
                     /translation="MAVVATRQGLSPVFRRRAPMRRHRCRLALTGASVLTVIFGLVFA
                     GALGVATMTPASAQSFTYNPRPPRAAPPRAASDGQMLVQATQVDFDYNTSRVSAVGNV
                     QMFYNGTSVEADKVIYDQKTKRLHAEGNIRMTDADGKITYANIMDLSDDYRDGFVDSL
                     RVDTADQTRMAATRADRSGGNYTVFENGVYTACAPCKDDPKKPPLWQVKGARIIHDQT
                     DKMLYFENAQLEFFGVPMAYLPYFSTPDPTVKRKTGFLMPAVSSATGYGYGVETPFYW
                     AIAPDYDATLNPRFTTRQGVLLQGEFRQRLLDGSYQIRAYGIDQLDPKAFAGQPGDRQ
                     FRGGVDTKGQFALNDKWVWGWDGVLLSDYFFFQDYKLAQYKDPLGSFLTLPTEAISQL
                     YLTGVGNRSFFDLRTIYYLSYSGNQSQVPIIHPVLDYSNVLNRNILGGEFSYKTNFTS
                     LTRDTAAFDPITTSANTQGLCTMASADPLARMPNQCLLRGMPGTYTRATAEVQWRRSF
                     TDPAGQIWTPFASLRADAIDASISNQPGVSDFLPVGDTQALRLMPTVGLEYRYPFINV
                     QPWGSTTIEPIAQVIIRPNEPYAGKLPNEDAQSLVFDTSNLFSVDKFSGYDRVEGGGR
                     ANVGVQATTQFDRGGSVKVLFGQSYQLFGLNSFAVADVTNTGLDSGLATPKSDYVGSA
                     SYSPNRTYTFSVRSRFDQATFNIQRFEAEGRANFDRWSVSMLYGNYAAQPELGYLTRR
                     EGLLGSASVKVAANWVVTGAARWDLEANKVNQYIVGAGYVDDCFVLAANYVTSYAYAT
                     AATTPPVLSHAFMLQIGLRTLASSSASASAGATGIQ"
     gene            complement(4578..>4896)
                     /locus_tag="VGC82_10400"
     CDS             complement(4578..>4896)
                     /locus_tag="VGC82_10400"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY0423722.1"
                     /translation="IRSSESSGFATAGYRLQYHKLLAQPFLLAAMVMLAAAVSLRFFR
                     FGGVQKMVLSGVGAGFLLYVLSKVTEDLSKAELMHPIAAAWLPVFVGGLTGFLALLYQ
                     EDG"
BASE COUNT          910 a         1467 c         1598 g          921 t
ORIGIN      
        1 tgatgacatc gcgcagcggc ggaaggtcgt cgatcgcgct catgcctgcg tcgtcgcggc
       61 gatgcgggcg gccaggcgca atgcggcgat caggctcgat ggattggcgc ggccggtgcc
      121 ggcgatgtcg aacgcggtgc catggtccgg cgaggtccgg atgaacggca agcccagcgt
      181 gacattgacc gcgtcatcga acgccagggt cttgaccggt atcagcgcct gatcgtgata
      241 catgcagatc gcgcagtcgt aggttttgcg cgcggcgtcg tggaacatgg tgtccgccgg
      301 tagcggaccc ctgacatcga tgccctcgcc gcgcaggatc tcgacggcag gcgctacgac
      361 ggcctggtcc tcggtgccga gcgagccgtc ctcgccggcg tggggattga ggccggatac
      421 cgcgaggcgg gggttggcga tgccgaagcg ggctttcata tcccccacca cgatgcgcgc
      481 agtcgtgacg atcaggtcgc ttgagagctg cgagatcgcg tcgcggagcg gaaggtggat
      541 cgttgcgggc accacggcga gggcagggga ccacagcatc atgaccggtt gcggcgcacg
      601 gccgccgccc gccgcgagtt cggcgagaaa ctcggtgtga ccgggatgcc ggaagccggc
      661 gcgatagaga acgttcttgg cgaccggatt ggtgacaacg gcgccggccc gcccggcgac
      721 gacatcgccg accgcctgac gaatggacgc gagcgctgcc tccgcgctcc tatcgtccgg
      781 ccggcccgga ttggccgtgg cggcaatgcc ggtcgccacc accggcaacg cgtcggcgaa
      841 tgcgtcgcgg gcttcttctg cgctgacccc ggcgagcttg acgtcgagcc cgagagcctt
      901 cgctcgaccg gccaggaagg cgcggtcgcc gagcagatag aatggcggca gcttgagctc
      961 attgcggcgt agccacgcct tgatggtgat atcaggcccg atgcctgcgg gctcacctaa
     1021 ggtcagcgcg aggggctttg ccatgcgtca acgatattcg atcatcgccg ctttgcggac
     1081 ttcctgcagg taggctttcg atttcgcctc gtacttgtcg gaaaacatct tgtcccggac
     1141 cgcctttttt ttcggcgagt cgacggtggt cggtttcctc ccgcacagcg caaccatctc
     1201 gacgccctgc ttggtcacct cgggcggcgt gaggtggccg atcggcgtct tgtcgagcag
     1261 gtcgcgaaga acaggcggta tgtcggcgga ggtcttgatc acggcttcgc ggatcgcggc
     1321 gttctgcatc gacttgaaga gcgcgttcgc gtcgtcgcag gtttgcacgc ggttacgcag
     1381 gacctcggct tccttctgcc gcgcctcgat ggcggcgggc gcggatcctc gcgggacgat
     1441 caggacgatc ggctgcatct tgtattcgaa ggcctcggcc tcctgcttct cgtcgccgct
     1501 gccctgcacc gccgcggcaa catccttctc gccgacctgg aggctctcct tgtagcgtcc
     1561 gcgaaccaga ctggtccaaa ccatctcggc cttaatgcgg gccttcagcg tatcagcccg
     1621 gatgccctgg ctctcaagag attttgtcag ctggtcgggc gtgatccgca tccgcgaact
     1681 catcgccgca aaggattgat cgatgtcgga gacgccagga tcgacgccaa atttcttgcc
     1741 ttccttgatc ttcactttct catcgatcaa ctcgttgatc acttgctttc ggtcggcagc
     1801 cttgtgggtc gtcaggaagt tcagcttgct gcgctgttcg atgtcgtaat tggtgatcgg
     1861 ctcgccgttg accatgaccg cgaccgtttg cgcacgcaac ggcgaagcgg tgcaggtcat
     1921 caggagcaac ccggcggcac aacccgcaac gagagtccga agccgatggg aggatgctgt
     1981 cgtcttcatg gtcatgttcg ctatatcaac cgattcgaat tgcagccgcg acgcgccggg
     2041 gattcgccct cgggcaccgt gccggcaccg gttctactgg atacccgtgg cgccggcgct
     2101 tgctgacgcg gacgaactgg caagcgtacg caggccgatc tgcagcatga aggcatggct
     2161 gagcacgggc ggcgtggtgg cggcggtggc gtaggcgtag gaggtgacat agttggcggc
     2221 cagcacgaag caatcgtcca catagccggc gccgacgatg tactgattga ccttgttggc
     2281 ttcgagatcc catcgggcgg ctcccgtcac cacccagttc gccgcaacct tgaccgacgc
     2341 gctgcccaaa agtccttcgc ggcgggtcag gtatccgagt tcgggttggg cggcgtagtt
     2401 gccgtaaagc atgctgaccg accagcggtc gaagttcgca cggccttcag cctcaaaccg
     2461 ctggatgtta aacgtcgctt gatcgaagcg cgaacgtacg ctgaaggtat aggttctgtt
     2521 gggcgaatag ctggcgctgc cgacataatc ggatttcggg gtggcaagac ccgaatcgag
     2581 accggtattg gtgacatcag cgaccgcgaa cgagttcagg ccgaatagct gataggactg
     2641 tccgaacagc accttgaccg agccgccgcg gtcgaattgc gtggtggcct ggacgccgac
     2701 attggcgcgg ccgccgccct cgacgcggtc atagcccgag aacttgtcga cgctgaacag
     2761 attgctggtg tcgaacacca gactttgcgc gtcttcgttg ggaagcttgc ccgcataggg
     2821 ttcattggga cgaatgatga cctgcgcgat cggctcgatt gtcgtgctgc cccacggctg
     2881 aacgttgatg aagggatagc gatattcaag accgaccgtg ggcatcaggc gcagggcttg
     2941 agtgtcaccc accgggagga agtccgaaac acccggctgg ttcgagatcg aggcatcgat
     3001 cgcatcggcg cggagactcg caaacggggt ccagatttgc cccgccgggt ccgtgaacga
     3061 gcgccgccac tgcacttcgg ccgtcgcccg cgtgtacgtg ccgggcattc cgcggagcag
     3121 acattggttc ggcatccgcg ccagaggatc ggcggacgcc atcgtacaca ggccttgggt
     3181 gttcgccgac gtcgtgatcg ggtcgaacgc ggccgtgtct cgagtcaggc tcgtgaaatt
     3241 cgtcttgtag ctgaattcgc cgcccaggat attgcggttg aggacattcg aataatcaag
     3301 gacaggatgg atgatcggca cctgggactg attgcccgaa tagctcagat aataaatcgt
     3361 gcggagatcg aagaagctcc ggttgccgac accggtcaga taaagctggg atatggcttc
     3421 cgtcggcagg gtgagaaaag agcccagcgg atccttgtat tgggccagct tgtaatcctg
     3481 gaagaagaag tagtccgaga gcagcacgcc gtcccagccc cacacccact tgtcgttcag
     3541 cgcgaactgg cccttggtgt cgacgccgcc gcggaactga cggtcaccgg gctggccggc
     3601 gaaggccttt ggatccaact ggtcgatacc gtaagcgcga atttgatacg atccgtccag
     3661 caggcgctga cggaattcgc cctgtaacag cacgccctgc cgggtggtaa agcgcggatt
     3721 gagggttgcg tcgtagtctg gggcgattgc ccagtagaac ggggtttcaa cgccataccc
     3781 atagcccgta gccgaagaga ccgccggcat caaaaacccg gtcttgcgct tcaccgtcgg
     3841 atcgggcgtc gagaaatacg gcagatacgc catcggaacg ccgaaaaact cgagctgtgc
     3901 gttctcgaag tagagcatct tgtcggtctg gtcgtgaatg atgcgggcgc ccttgacctg
     3961 ccacagcggc ggctttttcg gatcatcctt acagggcgcg caggcggtat agacaccgtt
     4021 ttcaaagacg gtgtagttgc cgcccgaacg gtcggcgcgc gtcgcggcca ttcgcgtctg
     4081 gtcggcggtg tcgacgcgca gcgaatcgac aaacccgtcg cggtaatcgt cgctgagatc
     4141 catgatgttg gcgtaggtga tcttgccgtc cgcatcggtc atgcggatgt tgccctcggc
     4201 atggagccgc ttggtcttct ggtcgtagat gaccttgtcg gcctcgacgc tggtcccgtt
     4261 gtagaacatc tgcacgttgc cgaccgccga cacccgtgaa gtgttgtagt cgaagtcgac
     4321 ttgggtcgcc tggaccagca tctgtccatc actggcggcc cggggcggcg ctgcgcgagg
     4381 aggtctggga ttataggtaa aggactgcgc ggaggcgggc gtcatagtcg ccaccccgag
     4441 cgcgccggca aaaaccaacc cgaaaatgac ggttagaacg gatgcgccgg tgagggccaa
     4501 gcgacaccga tggcggcgca tgggggcgcg ccgcctgaac acgggcgaca gtccttggcg
     4561 ggtggcgact acagccacta cccgtcctcc tggtacagca aggccaaaaa gccggtgagg
     4621 ccacccacga agacgggcag ccacgccgca gcgatcggat gcatcaactc agccttgctc
     4681 aaatcctcag ttactttcga cagaacatag agcagaaagc ccgcgcccac gccacttaaa
     4741 accatctttt ggacgccgcc gaagcggaag aaccgtaacg ataccgcggc ggccagcatg
     4801 accatcgcgg ccagcaaaaa cggctgtgcc agaagcttat ggtactgcaa ccggtacccg
     4861 gcggtcgcga atcccgagct ttcggaagac cggatg
//
LOCUS       DASWQN010000611         3938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1774543, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000611 DASWQN010000000
VERSION     DASWQN010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774543"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="VGC82_10405"
     CDS             <1..1289
                     /locus_tag="VGC82_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0423723.1"
                     /translation="AIASGMNIPIDISIVDSIPAPIAKGGPGAKLRTDGRGRNGMDFH
                     INPEQREMVASVRALAQSEFKATAGKWMDGTFPWENMKKLAELGVLGMSVPEEYGGLG
                     LPILDTALILEEIAKVDYVTAMAVLGEAGVQTRVIARYAPASIRERILPAVVSGDCIL
                     AVCMTEPHAGTDVANYRTNARIVGDRVVLKGTKTLISRAREAGMFVVFTRVDGKPGRE
                     GIGCVLLEPNTPGFEVTGTYHTMGGENLHEIQFNDCELPLENLVIREDGFRKLLSAFN
                     TQRCLNPAISLGLAEGAFDEAVNYVRERTVFNKPIADFQGIRWKLADMFKDIEAGRGL
                     LYRACLTANPFPDPFMAAAAKIFCNEMSLRVTTEAVQVHGGFGFTDEYPVSRFYRGAR
                     YGSIGGGASETLRDLIGKKIVGEFDVADGIMGLGTF"
     gene            1291..2412
                     /locus_tag="VGC82_10410"
     CDS             1291..2412
                     /locus_tag="VGC82_10410"
                     /inference="COORDINATES: protein motif:HMM:NF020031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY0423724.1"
                     /translation="MLLIEADGKTLFAEQSIPVPAAVLATDVVPPLPGEGPWIVKAQV
                     PAGGRGNAGGIIRCVSRAEVEAAVAGMLGTRLKGHDIDACLVESAVAGQERYLAIMVD
                     PPSYGLRVIYSAQGGVEIEQSGSIQGRLCAPHPGAASEALAELLVDEPDAWRSQVAGI
                     GKALATLLLERELALAEINPLFVSPEGCAAGDAKVIIDLNAVYRQPPIAAIIASRPRT
                     YADACRKLTEGFDYVELDPDGEIGLVTTGAGLSMMLIDELTARGAKPLNFCDIRTGQM
                     RGSPARLIRVLQWITARPSLKAVLVNIFAGITDLGEFAGLLAAAIQASPSLRVPVVAR
                     LVGRNAAEARRILLEAQPDMLVTEDLEEALARIDEVVRA"
     gene            2409..3293
                     /locus_tag="VGC82_10415"
     CDS             2409..3293
                     /locus_tag="VGC82_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY0423725.1"
                     /translation="MIGSLSRTTPVIVQGITGRMGSSHAALMRAYGTNIVGGTSIRTD
                     IPQAAGVPVFPTCAEAVVATGAVASIAMAPPEETWSAVREALAAGIKLIVTVAEGVPM
                     HDAIRIGRAVKRAGAVWVGASTPGLAVPGECKLGFLPDVCLRPGPIGIMSKSGTLSYE
                     VGYRLAREGLGQSIWVGVGGDPVKGVRFADMLPVFFSDPRTKGIILVGEVGGAEEEEC
                     AEAYARLGARKPLFALIAGREAKEGVSMGHAGALVHGDSGTLESKTKQLTRAGARVFG
                     SVEGLIGACLRDYRFDRR"
     gene            complement(3476..>3938)
                     /locus_tag="VGC82_10420"
     CDS             complement(3476..>3938)
                     /locus_tag="VGC82_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0423726.1"
                     /translation="VALYLPAAPPSLTHSTASEPDMADRRPARTESVFLVDDDVSVRE
                     VLSAELAQAGFTVVEAASGEAALQLVDSGLEFDVLVTDLAMPRMDGIALIRAIGVRRP
                     HARAIILTGNAGAGASLAIGGALPSVTLLRKPIAGQELADQIAALLEPAAS"
BASE COUNT          713 a         1190 c         1326 g          709 t
ORIGIN      
        1 gcgcgattgc gtctggcatg aatattccaa tcgatatttc cattgtcgat agcatcccgg
       61 cgccgattgc caaagggggg ccgggggcta agctgcgcac cgacggaagg ggacggaacg
      121 gcatggattt ccacatcaac ccggaacaac gcgagatggt cgcctcggtg cgcgcgttgg
      181 cgcagtccga gttcaaagcc acggccggca aatggatgga cggcacgttc ccctgggaga
      241 acatgaagaa actcgccgaa ctcggcgttc tgggcatgtc tgtgcccgag gaatacggcg
      301 gccttggcct gccaatcctg gacaccgcac tgatcctgga ggagatcgcc aaagtcgatt
      361 atgtcaccgc catggcggtc ctgggtgagg ccggcgtgca gacccgggtg atcgcccgct
      421 acgcgcctgc gtccatccgc gaacggatcc tgccggcggt ggtcagcggc gactgcatcc
      481 tggcggtctg catgacggag cctcatgccg gaacggacgt cgccaactac cgcaccaatg
      541 cccgcatcgt cggtgaccgc gtcgtcctga agggaaccaa gacgctgatc agccgagcac
      601 gggaggctgg gatgttcgtg gtgttcaccc gggtggatgg gaagccgggg cgggaaggga
      661 tcggctgcgt gctgctggag ccgaatacgc cggggttcga ggtcactggc acctaccaca
      721 ccatgggcgg cgaaaacctg cacgagatcc agttcaacga ctgcgaactg ccgctggaga
      781 acctggtgat ccgcgaggat ggatttcgca agctgctgtc ggcgttcaat acgcagcgct
      841 gcctcaaccc ggcaatctcc cttgggctgg ctgaaggcgc tttcgacgaa gccgtcaatt
      901 atgtccgcga acgcaccgtc ttcaacaagc cgatcgccga cttccagggc atccgctgga
      961 agctggctga catgttcaag gacatcgagg ccggccgcgg gctgctgtac cgcgcgtgcc
     1021 tgaccgccaa tccgtttccc gatccgttca tggctgcggc ggcgaaaatt ttctgcaacg
     1081 aaatgtcctt gcgcgtgaca accgaggcag tccaggtgca cggcggcttc ggcttcaccg
     1141 acgaataccc cgtttctcgc ttctaccgtg gcgcgcggta cggctcgatc ggcggcggcg
     1201 cgtcggagac actgcgcgat ctgatcggca agaagatcgt tggcgagttc gatgtcgcgg
     1261 atggcatcat ggggctgggg actttctgac atgctgctga tcgaggcgga cgggaaaacc
     1321 ctgttcgccg aacaaagtat tccagttccg gccgctgtgc tggcgaccga cgtcgtgccg
     1381 ccattgccgg gcgaggggcc gtggatcgtg aaagcgcagg tgccggccgg ggggcgcggc
     1441 aacgccggtg ggatcatccg ttgcgtttcc cgcgccgagg tcgaggccgc cgtcgccggc
     1501 atgttgggca ctcggctgaa gggccacgat atcgatgcgt gcctggtgga gtcggccgtg
     1561 gccgggcagg agcgctatct ggcgatcatg gtcgatccac ccagttacgg actgcgcgtg
     1621 atctattctg ctcaaggtgg cgtcgagatc gagcaatccg gttcaatcca aggccggcta
     1681 tgcgcgccgc atccgggcgc cgcatccgag gcgctggccg aactgctggt ggacgaaccc
     1741 gacgcgtggc gttcccaggt cgctggcatc ggcaaggcgc tggcaacgct gctgctggag
     1801 cgcgaattgg cgctcgccga gatcaacccg ctgttcgttt caccagaagg ttgtgcggcc
     1861 ggcgacgcca aagtgatcat cgatctcaat gccgtctatc gtcagccccc catcgccgcg
     1921 atcattgcct cccggccgcg cacctacgct gacgcatgcc gcaaactcac ggagggcttc
     1981 gattatgtcg aactcgaccc ggacggagaa atcgggctgg tcaccactgg cgccggcctt
     2041 tcgatgatgc tgatcgatga actgacggcc cgtggcgcta agccgctgaa tttttgcgac
     2101 atccgcaccg gccagatgcg cggcagccca gcacgcctga tccgggtttt gcagtggata
     2161 accgcccgac cctctttgaa ggcggtgctt gtcaacatct ttgccgggat caccgacctc
     2221 ggtgagttcg ccgggctgct ggccgccgcg atccaggctt cgccctcatt gcgcgttccg
     2281 gtggtggccc gtctggtcgg ccgtaacgcg gcggaagcac ggcggatcct gctcgaagcg
     2341 caaccggaca tgctggtgac cgaggatctc gaggaagcgc tcgcgcgcat cgacgaggtc
     2401 gtacgggcat gatcgggtcg ctgtcgcgga cgacgccggt gatcgtgcag ggcatcaccg
     2461 gacgcatggg aagcagccac gcggcgctga tgcgggctta tggaacaaac attgtcggcg
     2521 gaacctccat tcggaccgac atcccgcaag ccgccggcgt tcctgtgttt ccgacatgcg
     2581 ccgaggcggt ggttgcgacc ggagcggttg cctccatcgc gatggcgcct cccgaggaaa
     2641 cctggagcgc ggtacgggaa gccttggccg cggggatcaa gttgatcgtc acggtcgccg
     2701 aaggcgttcc gatgcacgat gcgatcagga tagggcgggc cgtcaagcgg gcaggggcgg
     2761 tttgggtcgg cgcctccaca ccgggtctgg cggtgccagg ggagtgcaag ctggggttcc
     2821 tgccggatgt ctgcctgcgc ccggggccga tcgggatcat gtcgaaaagt ggcactctgt
     2881 cctatgaagt cggctaccgc ctcgcacgcg aggggctcgg acagagcatt tgggttggcg
     2941 ttggtggcga tccggtcaaa ggcgttcgct ttgcggacat gctgccggtg tttttcagtg
     3001 atccacgaac gaagggaata atcctggtcg gcgaagtggg cggtgccgag gaagaagaat
     3061 gcgccgaggc ctacgcgcga ctgggtgcga gaaagccgct ctttgccctg atcgcggggc
     3121 gggaggcaaa ggaaggcgtt tccatgggcc acgccggcgc gctggttcat ggcgactccg
     3181 ggacgctgga atcgaagaca aagcaattga cccgggccgg cgccagggtc ttcgggtccg
     3241 tcgaaggcct gatcggtgcc tgccttcgag actatcgttt cgataggcgc taaacggcga
     3301 gcgggccgtt tagcgcattt tcagcccgac tatacaggtc gaaaatgctc tattctcttg
     3361 tttgatcgcg tgaatcaagc gcttcggacg ttccgcccag cgtgatcgcg ctgtaggcaa
     3421 taccgactat gatgaactgg atgttcaccg ccgtggcgga atcctggttc gaacgctatg
     3481 aagccgccgg ttccagcagg gctgcgatct ggtcggcaag ttcctgcccg gcgatcggct
     3541 tgcgcagcag agtaaccgat ggcaaggcac cgccgatggc cagtgaagca ccggcgccgg
     3601 cattgccggt caagatgatg gcacgtgcgt gcggccggcg caccccgatc gccctgataa
     3661 gcgcgatgcc atccatgcgc ggcatggcaa gatcggtgac cagcacgtca aattccaggc
     3721 cgctgtcaac cagttgcagc gctgcctccc cggatgcagc ctcgacaacg gtgaagccgg
     3781 cctgtgccag ttcagcagac aaaacttcgc gcacggacac atcgtcatca accagaaaga
     3841 cggactcggt tcgggcaggc cggcgatccg ccatgtcggg ctccgacgcg gtggaatgcg
     3901 tcaggcttgg cggcgccgcg ggcagataaa gcgcaacc
//
LOCUS       DASWQN010000612         4673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_323564, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000612 DASWQN010000000
VERSION     DASWQN010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323564"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..969
                     /locus_tag="VGC82_10425"
     CDS             145..969
                     /locus_tag="VGC82_10425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY0423727.1"
                     /translation="MQYGIHLPHAGEQASPALIRRFAIHAEELGLDDIWVSEHIIVPR
                     DKFPRSPLFYDPVLTLTWAAAVTTRVRLGTSVLVLPMRHPLPLAKELATLQNLSEGRL
                     ILGAGVGWLEPEFAALGVPFAERGRRLDEGLAMMRAVWTQDPVTFHSRTIPAVIEGMT
                     MTPMPVLPIPLWLGGSSDAALRRTVRVADGWHGSGLTPAAAAPIVQRLRAARPEAEFV
                     ISIRLHWNGRDPAVLKDAVEGFAAIGVQHIMIAPEDRNVDDWDSVIAGVGKLAAER"
     gene            1080..2087
                     /locus_tag="VGC82_10430"
     CDS             1080..2087
                     /locus_tag="VGC82_10430"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY0423728.1"
                     /translation="MANRRRFLQGSAGMGAALAAFGINPALAQEMGKAAGRSSKPLKA
                     AFSNAGLQATWCAQGKQAAEYWGKLFNVEVTWFDGELAAQKQRAAIDNLASQKWDFVA
                     IQAFGIGTLTAPVNKLIDAGTPVIDMDTLIAPLDTINIHSFLAPDNEFMGASVTQALV
                     DSIGGEGTMIMTQGALGHTGAQGRARGFQSVVKKYPKIEVLDTQPADWDVTKVARIWE
                     TLLTKYPKISAAYFHNDDMALAAHNVMKAHNRTEIKIGGCDAMPPALAAVSDGRMLAT
                     VRNPSCRIHGGAVVAGVAAVMAGEKTGSSGIPKNIVVDGPVVTKANAPGMMWMEEHFL
                     I"
     gene            2095..3618
                     /locus_tag="VGC82_10435"
     CDS             2095..3618
                     /locus_tag="VGC82_10435"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY0423729.1"
                     /translation="MSDLKAPTLLKAPALLELSGITKTFGGVAALREVDFSLLAGEIH
                     GLVGENGAGKSTLMKIIAGVHAEYQGEMRLDGQTVRFASPRDARAGGIGMVHQELSVV
                     PSLSVAENVHLGSQPVHRFGLIDWRAMRRGAAEHLANLGIDADPRAPMGGLPLGLQQL
                     VELARVLFSGARIIILDEPTSALAPPEVERLFGVLRRLRDAGRSIVFISHFLDDVLAV
                     CDRVTVFRNGQAVATEACRHVSKRWVIDRMIGTGHGELEESYLGDITLHSSPEARVVL
                     EVEGLHRAGAYHDVSLRVRAGEVLGIYGFLGAGQLEFARTLFGLLPADRGRIALEGRM
                     VRFASAPQARRMGMAFLPESRRLMLFGSEPVFKNVTIAILERIHRLWLRPGAERAIAE
                     RHVQGLRIRPPDVSRALGTLSGGNQQKVALAKWLTHLPRVLVLSEPTRGMDIAAKDDV
                     IRIVRGLREQGIAIVVVSTEPETVLSLADRILVMKKGRVVREFANEPVSKDRLMEAA"
     gene            3615..4628
                     /locus_tag="VGC82_10440"
     CDS             3615..4628
                     /locus_tag="VGC82_10440"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0423730.1"
                     /translation="MSGTVQAARTGLLGWLRGRLRNVAPFVTLLLLIAFFSLASGSFA
                     TLGNLQNILTQVSVTAIIAVGLTFVILCAEIDLSVASIANATGIVVAYFTMQDPSVTI
                     ANIPLPGWAAIVIALSACFALGAVNAFGLTRIGIPSFIMTLAMLQIAAGICALLVRGQ
                     IAYAVPPLIGTLGARSFGPVPWIVIVAAAFLLLGHIVLTYTRFGRYVYMTGGNREAAE
                     YSGVNVRAILSAVMIISAVCSGVAGMLGVAYFGSAQQNEFDTYLLDSISAVVVGGTSL
                     FGGQGGIGNTIIGLFVLGILNNGLDHINIDSFLKILIRGLILLVALVINVYAQRMRTG
                     ETK"
BASE COUNT          770 a         1427 c         1625 g          851 t
ORIGIN      
        1 cgcactgtca accggcggtg taggaggaag ccacggagcg tgcggtatcg ccgttttgcc
       61 cgaacctcta ctcttgttcg aggcgacgtt ccgctgcggc gatatgcgac ccggcgatat
      121 gtgacccatg gaggaaatcc aacgatgcaa tatggcattc acctgccgca tgccggcgaa
      181 caggccagtc cggcgctgat ccggcggttc gcgatccacg cggaggagct gggtctcgac
      241 gacatctggg tgagcgagca catcattgtg ccgcgggaca aatttccccg ctccccgctg
      301 ttctacgacc cggtgctgac cctgacctgg gctgccgcgg ttacaacgcg cgtgcggctt
      361 ggcaccagtg tcctggtgct gccgatgcgc cacccgctgc cgctggcgaa ggaactcgcc
      421 accctgcaaa acctgtcgga gggccgtctg atcctgggtg cgggcgtcgg ttggctggag
      481 ccggaattcg ccgccctggg cgtgccgttc gccgaacggg gccggcggtt ggacgaggga
      541 ctggcgatga tgcgggccgt ttggacccag gatccggtga cgttccacag ccgcacgatc
      601 ccagccgtga tcgagggcat gacaatgacg ccgatgcccg tccttccgat tccgctctgg
      661 ctgggcggca gctccgacgc ggcattgcgc cgcacggttc gggtcgccga cggctggcat
      721 gggtctggcc tgacgcccgc cgccgccgcc ccgatcgttc agcgtctgcg cgccgcgcgg
      781 cccgaggccg agttcgtgat ttcgatccgc ctgcactgga acggccgtga tccggccgta
      841 ctgaaggacg cggtcgaagg cttcgcggcg atcggcgtgc aacacatcat gatcgctccg
      901 gaagaccgca atgtggacga ctgggacagc gtgatagccg gcgtcggaaa gcttgcggcg
      961 gagcggtaga aggccgttcg ccttccgatc gccgaacctg tggtatctgc tgccatcggg
     1021 ggttgccgcg aatcacgccg ggggattacc gtccggcaat aaaaccggga gggataacca
     1081 tggccaaccg caggcggttt ctacagggtt cggcggggat gggcgcggcg ctcgcggcat
     1141 tcggtatcaa cccggcgctg gcgcaggaga tgggcaaggc cgcggggcgg tcgagcaaac
     1201 cgctcaaggc cgcattctcg aacgccggct tgcaggcaac ctggtgcgct cagggcaagc
     1261 aggcggcgga atactggggc aagttgttca acgtcgaggt gacctggttt gacggtgaac
     1321 ttgccgcaca gaagcagcgt gcggcgatcg acaacctggc gtcgcagaag tgggattttg
     1381 ttgccatcca ggccttcggg atcggcacgc tgaccgcccc ggtcaacaag ctgatcgatg
     1441 cgggaacccc cgttatcgac atggacacgc tgatcgcgcc cctcgatacc atcaacatcc
     1501 acagcttcct tgcgcccgac aatgaattca tgggcgcatc cgtcacccag gcgctggtgg
     1561 attcgatcgg cggcgaaggg accatgatca tgacccaggg tgcattgggc cataccggcg
     1621 cgcaggggcg ggcccgcggc ttccagtcgg tggtgaagaa ataccccaag atcgaggtgt
     1681 tggacaccca gccggccgac tgggatgtga cgaaagtggc acgcatctgg gaaacgctgc
     1741 tgaccaagta tccgaagatc agcgccgcgt atttccacaa cgacgacatg gcgctggcgg
     1801 cgcacaacgt gatgaaggcg cataaccgca ccgagatcaa aatcggcggc tgcgacgcga
     1861 tgccgccggc gcttgccgcg gtcagcgatg gccgcatgct ggcaacggtg cgcaatccgt
     1921 cctgccgcat ccatggcggc gcggtcgtgg ccggtgtcgc cgcggtgatg gccggcgaga
     1981 agaccggttc cagcggcatc ccgaagaaca tcgtggttga cgggccggtc gtcaccaagg
     2041 cgaatgctcc gggcatgatg tggatggagg aacacttcct gatctgaccg gccggtgtct
     2101 gatttgaagg cgcccacgct tttgaaggcg cccgcgctac tggaactgtc cggcatcacc
     2161 aagaccttcg gcggcgtcgc ggccttgcgc gaggtggact tttccctgct ggccggcgaa
     2221 atccacggat tggtcggcga aaacggcgcg ggcaaatcca cgctgatgaa gatcattgcg
     2281 ggcgttcacg ccgagtatca gggcgagatg cggctcgatg ggcaaaccgt gcgcttcgcc
     2341 tcgccgcgcg atgcacgggc cggcggaatt ggcatggtgc accaggaatt gtcggtggtg
     2401 cctagcctga gcgtggcgga gaacgtgcat ctcggctcgc agcccgtgca tcgcttcggg
     2461 ttgatcgact ggcgagccat gcggcgcggc gcggcggagc atctggcgaa cctcggtatc
     2521 gacgccgatc cccgagcacc catgggcggg ctgccgctcg ggctgcagca gctcgtcgaa
     2581 ctggcgcggg tgctgttttc gggcgcccgc atcatcatcc tggatgagcc gacgtccgcc
     2641 ctcgcgccgc cggaggtgga gcggcttttc ggtgttctgc gccggctgcg cgatgcgggc
     2701 agaagcatcg ttttcatctc gcattttctc gacgacgtgc tggcggtctg cgaccgggtg
     2761 acggtgttcc gcaacggcca ggccgtggcg accgaagcct gtcgccacgt cagcaagcgc
     2821 tgggtcatcg atcggatgat cggcaccggg cacggagagc tggaggaaag ctacctcggc
     2881 gacatcacgc tgcacagctc gcccgaggca cgggtggtgc tggaagtcga aggcctgcat
     2941 cgtgccggcg cctatcacga cgtctcgctg cgggtgcggg ctggtgaggt tctggggatc
     3001 tacgggttcc tcggcgccgg gcagttggag tttgcccgca cgttgttcgg cctgctgccg
     3061 gccgatcgcg gacgcatcgc gctggagggc cggatggttc ggttcgcctc ggcaccacag
     3121 gcgcggcgga tggggatggc gttcctgccg gagagccggc ggctgatgct gttcggttcg
     3181 gagccggtgt tcaagaacgt gacgatcgcc atcctggagc ggatccatcg tttgtggctg
     3241 cgcccgggcg ccgaacgcgc gatcgccgaa cgccacgtcc agggacttcg catacgcccg
     3301 cccgacgtgt ctcgcgcctt gggcacgctg tccggcggca accagcagaa ggtggcgctg
     3361 gcaaagtggc tgacgcacct gccgcgcgtg ctggtgctga gcgagcccac gcgagggatg
     3421 gacatcgccg ccaaggacga cgtgatcagg atcgtgcgtg gcctgcgcga acaggggatc
     3481 gcgatcgtgg tggtttcgac cgaacccgaa acggtgctgt cgctggcaga ccgcatcctg
     3541 gtgatgaaga agggacgcgt ggtgcgggag ttcgccaatg agccagtcag caaggaccga
     3601 ctgatggaag ccgcatgagc ggcaccgtgc aagccgcgcg cacgggtctg ctggggtggc
     3661 tgcgcggacg gttgcgcaat gtcgccccgt tcgtgacctt gctgttgctg atcgcgttct
     3721 tttccctcgc gagcggctcg tttgcgacac tgggcaacct gcaaaacatc ctgacgcagg
     3781 tctcggtgac cgcgatcatt gcggtggggc tgacgtttgt gatcctgtgc gccgagatcg
     3841 acctgtcggt cgccagcatc gccaatgcga ccggtatcgt cgtggcatat ttcacgatgc
     3901 aggatccgag tgtcacgatc gccaacatcc cgctgccagg ctgggcggcg atcgttatcg
     3961 cgttatcggc gtgcttcgcc cttggcgcgg tgaatgcgtt cgggctgacg cgcattggca
     4021 ttcccagctt catcatgacg ctggctatgc tgcagatcgc cgccgggatc tgcgcgctgc
     4081 tggttcgcgg acagatcgcg tacgcggtgc cgccgttgat cgggacgctg ggcgcacgct
     4141 cgtttggccc cgtcccgtgg atcgtgatcg tggccgccgc gttcctgttg ctgggccata
     4201 tcgttctgac ctacacgcgg tttggccgct atgtttacat gaccggcggc aaccgcgagg
     4261 ccgccgagta ttccggcgtc aatgtccgag cgatcctgtc ggcggtgatg atcatctcgg
     4321 ccgtgtgttc cggcgtcgcc gggatgctgg gcgtggcgta cttcggcagc gcgcagcaga
     4381 acgaattcga cacctacctg ctcgacagta tttctgcagt ggtggtgggc ggcaccagcc
     4441 tgttcggcgg ccagggcggc atcggcaata ccatcatcgg gctattcgtg ctggggatcc
     4501 tgaacaacgg gctggaccac atcaacatcg acagcttcct gaagatcctg atccgcggcc
     4561 tcattctgtt ggtggcgctg gtgatcaacg tgtacgcgca gcggatgcgg acgggcgaga
     4621 cgaaatagcg gtgctggcgg cagcgttcag gaacggctgg ccggtgatgc tga
//
LOCUS       DASWQN010000613         2815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_718172, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000613 DASWQN010000000
VERSION     DASWQN010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718172"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..2508
                     /locus_tag="VGC82_10445"
     CDS             196..2508
                     /locus_tag="VGC82_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEY0423731.1"
                     /translation="MERKLLWLSAWMIHNANHIRPNRDGLKVGGHQASCASCISIMAA
                     LYLEVARPQDRIAVKPHAAPVFHAMNYLFGRQTREKMEGLRRFGGIQPYPSRVKDGGE
                     VDFSTGSVGLGVAMTSFAALMADYTRLHGLGRTDLPPGRHIAICGDAELDEGNIYEAL
                     LEGWKHDVRNVWWIVDYNRQSLDSVVPDRLFGRIEGLFRDMGWNCVTIKYGRKLEAAF
                     TREGGEALRAWIDDCPNSLYSALTFQGGAAWRQTLLSDLGRSKARAIIDELNDDELGA
                     LMTNLGGHDIETIIEAFRTADAEGDQPTCFIAYTIKGMGLPFAGHKDNHAGLMTKEQV
                     ELFRQEMGIRPGHEWDLFEGLDGSEAEFREFLAECPFGTPLTPEGRLLSAPAVHVPPG
                     LPMPRSAGRKMSTQAGFGDILAEIGRGQGELKDLAAHIMTTSPDVTVSTNLGPWVNRR
                     GIFDRHTRNDVFRDSKLASAQRWGMSPKGQHIELGIAEQNLFLLLGAAGLAPSMSGAR
                     VLPVGTVYDPFVNRGLDALIYACYQDARFLLISTPSGLTLAPEGGQHQSVNTPLIGMA
                     ADRLASFEPAHVDELGILLRHAFDFMQQPDGSAVWLRLSTRALEQKERSLDPSSVIAG
                     AHWVVPPVAGARIALAYQGPVAPEAEAAFAELVVEEPGAGLLAITSPDRLHAGWLAAQ
                     RARRTGDRGAMSHIETLLAPLARGAALVSVLDGHPATHAWLGSIRGQRVVPLGPDRFG
                     QGGDIPDLYREYGLDTDAILDACAQAMLAG"
     gene            2692..2768
                     /locus_tag="VGC82_10450"
     tRNA            2692..2768
                     /locus_tag="VGC82_10450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2726..2728,aa:Arg,seq:tct)
BASE COUNT          482 a          830 c          998 g          505 t
ORIGIN      
        1 gacggtcagc ccggaattcg gtcctctcac gccgcaaaac catcaggttt cgcagtctgg
       61 tgactccaac cccgaacagt gccaaaaccg ccgggtgctg aacatcgccg ggaggatttc
      121 cgaatgaacg cgccgattac cgcgatcgcg ccgctcgcac cgccccggtc acggcatgtg
      181 cacatcctga agcagatgga acgcaagctg ctgtggctgt cggcatggat gatccacaac
      241 gcgaaccaca tccgcccaaa ccgcgacggg ctgaaggtgg ggggacatca ggcgtcctgt
      301 gcgtcctgta tctccatcat ggcggcgctg tatctggagg tcgcccggcc gcaggatcgg
      361 atcgcggtga agccccatgc ggcgccggtg ttccatgcga tgaactatct gttcgggcgt
      421 cagacccgcg agaagatgga gggtttgcgg cggttcggcg ggatccagcc gtatccgtcc
      481 cgggtgaagg atggcggcga ggtcgatttt tccaccgggt ccgtcggcct gggagtcgcg
      541 atgaccagct ttgccgcgtt gatggcggac tacaccaggc tgcatggact ggggcggacg
      601 gacctgccgc cggggcgcca tatcgcgatc tgcggcgacg ccgaactgga cgagggcaat
      661 atctatgaag ccctgctgga gggctggaaa cacgatgtcc gcaatgtttg gtggatcgtg
      721 gattacaacc gccagagcct cgattccgtt gtgccggacc ggctgttcgg gcgcatcgag
      781 gggctgttcc gcgacatggg ctggaactgc gtcaccatca aatacggccg aaagctggag
      841 gcggcgttca ccagggaggg tggcgaggcg ctgcgcgcct ggatcgatga ctgccccaac
      901 agcctgtact cggcgctgac gttccagggc ggggcggcat ggcggcagac cctgctgtcg
      961 gacctgggac gctcgaaggc cagggccatt atcgacgaac tgaacgacga cgaacttggc
     1021 gcgctgatga ccaacctcgg tggccacgac atcgagacga tcatcgaggc gttccgcacc
     1081 gccgacgcgg agggcgacca gccgacctgc ttcatcgcct acacgatcaa gggcatgggc
     1141 ctgccgttcg ctgggcacaa ggacaaccac gccggactga tgacgaagga gcaggtcgaa
     1201 ctgttccgcc aggaaatggg gatcaggccc ggccacgaat gggacctgtt cgaaggcctc
     1261 gacggctccg aggcggaatt tcgagaattc ctggcggagt gtccgttcgg tacgcctttg
     1321 acccccgaag gtcggctgct gtcggccccg gcggtgcatg tgccgccggg gctgccgatg
     1381 ccccgatcgg ctgggcggaa aatgtcaacc caggccggct tcggcgatat cctggcggag
     1441 atcggacgcg ggcaggggga gctgaaggac ctcgctgcgc acatcatgac gacgtcgccg
     1501 gatgtcacgg tctccaccaa tttggggccg tgggtcaacc ggcgggggat tttcgaccgt
     1561 cacacgcgca acgatgtgtt ccgcgattcg aaactggcgt cggcacagcg ttgggggatg
     1621 tcgcccaagg ggcagcatat cgagcttggg atagccgaac agaacctttt tctgctgttg
     1681 ggcgcggcgg gactggcccc ttcgatgtcg ggtgcgaggg tcttgccggt gggcacggtt
     1741 tacgatccgt tcgtcaaccg cggtctcgat gccctgatct acgcttgcta ccaggatgct
     1801 cggttcctgc tgatctccac cccgtccggc ctgaccttgg cgccggaggg ggggcagcac
     1861 cagtcggtga acacgccgct gatcgggatg gcggccgatc ggctggcgag cttcgagccg
     1921 gcgcatgtcg atgaactggg catcctgctg cgccatgcgt tcgatttcat gcagcagcct
     1981 gacgggtcgg cggtttggct gcggctttcg acgcgggcgc tggagcagaa ggaacggagc
     2041 ctcgacccct cctcggtgat cgcgggcgcg cactgggtgg tgccaccggt tgcgggagcg
     2101 cggatcgcac tggcctatca gggaccggtg gcacccgagg ccgaggcggc tttcgccgaa
     2161 ctggtcgtgg aggagcctgg ggctgggttg ctggcgatta cctcgccgga ccggctacac
     2221 gccgggtggc tggcggcgca gcgagctcga cggaccggtg atcgcggggc gatgtcgcat
     2281 atcgagacgt tgctggcgcc tctggcgcgc ggagccgcgc ttgttagcgt gctggacggg
     2341 cacccggcaa cgcatgcctg gctgggttcg atccgcgggc agcgcgtggt gccgttggga
     2401 ccggaccggt tcgggcaagg tggggacatt ccggatttat accgggagta tggactggat
     2461 acggacgcga tcctggatgc gtgtgcccag gcgatgctgg cggggtgatg ggcaatcgaa
     2521 atggcggata actttgctct gcccaggacg gttcggacga gtcgcgaagt gcccgcgaaa
     2581 gacggtcggg tttacgcggg gaagtacgaa ttggcacaat cggactggtc gggtaggtat
     2641 tgcacgcccg gtaggctgca ttacgggttg gccggatagg ctggcgtacg ggcgccctta
     2701 gctcagttgg atagagcaac agccttctaa gctgtgggtc ggtggttcga atccaccagg
     2761 gcgcgccata acccatctgg ctcgtgtcat cgcccgctca ttccgccacc atcgg
//
LOCUS       DASWQN010000614         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2302542, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000614 DASWQN010000000
VERSION     DASWQN010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2302542"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VGC82_10455"
     CDS             complement(<1..1032)
                     /locus_tag="VGC82_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423732.1"
                     /translation="MMSQSVTQAVRPINLSPKPMVSAISLLLLSFVLGTVMFIALRSP
                     LKDDIAWLLYVAGRWMAGRELYIDLIEVNPPLIVWISAVPLQLAQWLDVGTQFVAIPV
                     FVAAVLGCGWWASGLLCVRGGIFADRLPVFAAIGTSLLILPAADLGQREHLLVAAFLP
                     YLVLFARSLEGKPVPVIASLAAGVLAGLGCALKPRYAAVFAVLECLALTRGVRPWRVM
                     PFAAGATLAGYAGLVALLCPAYLRRAVPMALALYGATDVPFLTLLADSALLLSGQAVA
                     VVLLWLRRRTLTDYSPMLTLLVFAATSTVICFVDGKDWYYHRLPATVATVLALAIWIA
                     AEVTQQRGRM"
     gene            complement(1177..1539)
                     /locus_tag="VGC82_10460"
     CDS             complement(1177..1539)
                     /locus_tag="VGC82_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0423733.1"
                     /translation="MYTVLVVEDEMLVRMPIAEHLRDCGYHVLEAGNASEAIAAMEGE
                     DPVSLVFTDVRMPGKMDGFGLAEWFHLHYPEVPVLLTSGYNGGRTIPTTGVDAGRFIE
                     KPYSQTQVAQQIAALLNT"
BASE COUNT          404 a          595 c          555 g          279 t
ORIGIN      
        1 cattctgccc cgctgctggg tcacctcggc cgcgatccag atcgccagcg ccaggaccgt
       61 cgccacggtt gccggcagcc ggtggtagta ccagtccttg ccgtcaacga aacagatcac
      121 cgtgctggtc gcggcgaaca ccagcagcgt gagcatcggg ctgtagtcgg tcagcgtgcg
      181 gcggcgcaac cacagcagga cgaccgccac cgcctgtccc gacagcagca aagcgctgtc
      241 ggccagcagg gtcaggaacg gcacgtcggt cgcaccgtac aaggcgaggg ccatcggcac
      301 cgcacggcgc agataggcgg gacaaagcag cgcgaccaag ccggcataac ccgccagcgt
      361 ggcgccggcg gcaaacggca tgacccgcca ggggcgcaca ccgcgcgtca gggccaggca
      421 ttccagtacg gcgaagaccg ccgcgtaccg cggcttcaac gcgcaaccca gacccgcgag
      481 cacccctgcc gccagggatg cgatgaccgg cactggtttg ccctccaggg accgggcgaa
      541 cagcaccagg tacggcagga acgcagcgac caggagatgc tcccgctgcc ccaggtcggc
      601 ggcgggcagg atcagcagcg acgtcccgat cgcggcgaat accggcaacc gatcggcgaa
      661 tatgcctccg cgcacgcaca acaggccgga agcccaccag ccgcatccaa gtacggcggc
      721 cacgaaaacg ggaatcgcga cgaattgggt gccgacatca agccactggg caagctgcag
      781 cggcacagcc gatatccaga cgatcagcgg cggattgacc tcgatgaggt cgatgtacag
      841 ctcccgtccc gccatccagc ggcctgccac gtacagcaac catgcgatgt cgtccttcag
      901 cggcgaccgc agggcgatga acatcaccgt gccgaggacg aacgacagca aaagcaagct
      961 gatggcggac accatcggct tgggcgagag gttgatcggg cggacggcct gggttacgga
     1021 ctgggacatc atggatcgat ctaaggcaaa tgactaaaaa gcggacgtat atcgcacgct
     1081 ttgttgccat ggtgttagag cgaatccaga tggcttaata atggcggcga ccgccgcgag
     1141 gccgaaaccc gggtggaacc aacacctggg gacggatcaa gtattcagca gcgccgcaat
     1201 ctgctgggca acctgggttt gcgaatacgg cttctcgatg aagcggccgg catcgacacc
     1261 ggtcgtcggg atagttcgcc cgccattgta gcccgaggtc agcaggacag gcacctcggg
     1321 ataatgcaaa tgaaaccact ctgccaaacc gaatccatcc atcttgccgg gcatgcgaac
     1381 gtcggtgaat accaagctga ccggatcctc gccttccatg gctgcgatgg cttccgacgc
     1441 gttcccagcc tctagcacat ggtagccgca atcacgcaga tgctcggcga tcggcattcg
     1501 caccagcatc tcgtcctcga caacgagaac cgtgtacatg caatggtttt ccaaaaagcc
     1561 gcgacccagc gaaaatgaca cgttattcat aagtctcgat gtggtgcccc cgcctgctaa
     1621 atgccgacga caccggggca acttatcgtg acattagaat ggcttcaact ttcggggatc
     1681 gcctaggcgc gaccgcggat cgaatctggc gcggcttatg tcgtccagaa catgctgttc
     1741 gtgcttggcg ccctcgactt cagcctcggc cagtcgctcg ccgatcagag tctcgacact
     1801 tttcgctgca atccgggcat tcacgaccgc gtc
//
LOCUS       DASWQN010000615         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1379388, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000615 DASWQN010000000
VERSION     DASWQN010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379388"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2852
                     /locus_tag="VGC82_10465"
     CDS             <1..>2852
                     /locus_tag="VGC82_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY0423734.1"
                     /translation="ICMEVSVGSKVAARGLEWDVTGIEQLGPQQLVRLACSGGDLAGL
                     EWNVLYPLEPLSIVRIDPEPDEAGPLDAWRRYHVARLLEQIPGVPVPPGRVAIEPYQR
                     VPLLRALDMVRPRLLLADGVGLGKTIQAGLIAAELLVRRRAHRILIVCPPGPLLRQWE
                     QEMRVRFGLRFTPISDSSSLRDARRGLELGGNPFDATALCLTSIDFAKQDHVLAELER
                     VAWDLVIIDEAHHCVGEENQRRLLAEVLSARADGLLLLTATPHDGHDPHFASLMALLD
                     PSLVDGAGRLAGTAYRRHVVRRLKSHIRTPSGAPLFRERIVIPVRVDVTHAPAVLDFH
                     KALSALVAPRLQRSRDRNGLTDALAFVSLLKRSMSTIAACVATLRVVAERYGTKPLRE
                     RRRALRAYRQRIVRYGVLGAAEDADLAELEAEEMAAGLAGAGTADALRHLIHLGETAA
                     AHDPKLAALVLEVRLIRLRHPGANILVYTEYADSQRAATEALAGIGGTVLTISGADSE
                     ADRTRAAERCTEEDGILLVSTDSLAEGLNLHQRCFHLIHLDLPYNPNRLEQRNGRIDR
                     YGQRNDPEIRYLFLAGTFEERLLLRLIAKYEKARACLSFMPNTLGVGADPVGLHEPLF
                     QGFREDLLSTLPRLVHSLDLAAEDTDSDAYRDLLREIDRAFQSFDDMAVRHGWLSGGE
                     TGDNPPAERCDNIDLVAFVRSVLPREEGGYRVPAGWMHDLENLPGFDPATGIVRLTED
                     PEQVRDERGRVLLYPGRSHPIVRRCIASVLTGRVSAARADTLSLLMTYVVEAGSQLRS
                     VFALRLFPDGSIVEQGDFLSLAGDAVSPNLLWGRLFAGWAPCAIAAAAGTAFKIADRI
                     GGRAAAVYQDQFDRDGAVAHAWLTRRGIELGGPVLRQTADLFDASPRRSDWRSCAIPE
                     QRLAGLVADPLMPAAHRRDAAEALLRFRARTAEMPP"
BASE COUNT          422 a          907 c         1001 g          522 t
ORIGIN      
        1 gaatctgcat ggaagtctcg gtcgggagca aggtcgccgc gcgcggtctg gagtgggatg
       61 ttaccggaat cgagcaactc ggcccgcagc agcttgtgcg gctggcctgt tccggcggcg
      121 atctggccgg cctggaatgg aacgtgctgt atccgctgga gccgctatcg atcgttcgta
      181 tcgacccgga acccgacgaa gccggcccgt tggatgcgtg gcggcggtat catgtcgcgc
      241 ggctgctcga gcaaattcct ggtgtgcccg ttcctcccgg gcgggtggcg atcgagccct
      301 accagcgagt cccgctgctc cgcgcgctgg acatggtgcg gccgaggttg ctgctggcgg
      361 atggcgtcgg cctggggaag accatccagg ctggcctgat cgcggcggag ttgctggtgc
      421 ggcggcgcgc ccaccggatc ctgatcgtct gcccgcccgg cccgttgctc cggcagtggg
      481 agcaagagat gcgggtccgc ttcggtctgc gcttcacccc catctccgac agctccagcc
      541 tgcgggacgc tcggcggggg ctggaactgg gcggcaaccc gttcgacgcc accgcgctgt
      601 gcctgacgtc gatcgacttc gccaagcagg accacgtact cgcggaactg gaacgcgtgg
      661 cgtgggacct tgtcattatc gatgaggcgc atcattgcgt cggcgaggaa aaccagcggc
      721 ggcttctggc cgaagttctg tcggcccgcg ccgacggttt gctgctgctg accgcaacgc
      781 cgcatgacgg acacgacccg catttcgcgt cgttgatggc gctgctggat cccagcctgg
      841 ttgacggtgc cggacggttg gccgggacgg cttatcggcg gcatgtggtc cggcggctga
      901 agtcccatat ccgcacaccg tcgggcgcac cgcttttccg ggaacggatc gtcattccgg
      961 ttcgcgtcga tgtgacgcat gcgcccgccg tgcttgattt ccataaagcg ttgtcggcct
     1021 tggtggcgcc caggttgcag cggtcgcgcg accggaacgg gctgaccgac gccctggcat
     1081 tcgtcagcct attgaaacgg tcgatgtcca ccatcgccgc ctgcgttgcg acgcttcggg
     1141 tggtggcgga gcgctatggc accaagccgc tgcgggagcg gcgtcgcgca ttgcgcgcct
     1201 atcgccagcg gatagttcga tatggtgtgc tgggagccgc cgaggacgcc gacctcgccg
     1261 aactggaggc cgaggaaatg gctgccggcc ttgcgggcgc cgggaccgcg gacgccttgc
     1321 gccacttgat ccacctgggc gagacggcgg cggcgcatga cccgaagctg gccgccctgg
     1381 tgctggaagt caggctgatc cggttgcgtc accccggcgc caacatcctg gtttatacgg
     1441 agtacgccga tagtcagcgt gcggcgacgg aggcgcttgc cggcatcggc gggacggtct
     1501 tgacgatcag cggtgcggac tccgaagcgg accgaacgcg ggcggcggag cgctgcaccg
     1561 aggaggacgg aatcctgctg gtcagcaccg attcgctggc cgaggggctg aacctgcacc
     1621 agcgctgctt tcacctgatt cacctcgacc tgccctataa tccgaaccgc ctggagcagc
     1681 gcaacggccg catcgaccgc tacggacagc ggaacgatcc tgagatccgc tacctgtttc
     1741 tcgccggcac cttcgaggaa cggctgctgc ttcggctgat cgcgaaatac gaaaaagcgc
     1801 gggcctgcct gtcgttcatg ccgaacacgc tgggcgtggg cgccgatccg gtcggtctgc
     1861 acgagccatt gtttcagggc ttccgcgagg atctgctcag cacgctgcct cggttggtcc
     1921 attcgctgga tctggcggcc gaggataccg acagcgacgc ctaccgtgac ctgctgcggg
     1981 aaatcgaccg cgcgtttcaa agcttcgatg acatggcggt ccgtcacggg tggttgtccg
     2041 gcggcgagac cggcgacaat ccgccggccg aacgatgcga taacatagat ctggtcgcgt
     2101 tcgtccggtc ggtgctgccg cgcgaggaag gtggctacag ggttcccgcc ggctggatgc
     2161 atgacctgga gaacctgccg ggcttcgatc cggcaaccgg gatcgtgcgg ctgacggaag
     2221 atcccgaaca ggtcagggat gagcgggggc gggtgctgct gtaccccggg cggtcccatc
     2281 cgatcgtccg gcggtgtatc gcgtcggtcc tgacgggacg ggtaagcgct gcgcgcgccg
     2341 acacattgtc gttgctgatg acctatgtgg tggaggctgg ctcgcagctc cggtcggtct
     2401 tcgcgcttcg cctgtttccg gatggttcca ttgtggagca gggcgatttt ctttcgctcg
     2461 ccggtgatgc cgtcagcccc aacctgttgt ggggccgcct gttcgcgggc tgggcgcctt
     2521 gcgccatcgc cgccgcggcc ggtaccgctt tcaagatcgc ggaccggatc ggcggccggg
     2581 cggctgccgt atatcaggac cagttcgacc gcgacggtgc ggtagcccac gcctggttga
     2641 cccggcgggg gatcgagctt ggcggaccgg tgcttcgtca gaccgctgac ctgttcgatg
     2701 ccagcccgcg gcgaagcgac tggcgatcct gtgccatacc cgaacagcgg cttgccggcc
     2761 tggttgccga tccattgatg ccggcggcac atcgccggga cgcagccgaa gccttgcttc
     2821 gattccgtgc ccggacggcg gaaatgccgc cg
//
LOCUS       DASWQN010000616         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1907128, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000616 DASWQN010000000
VERSION     DASWQN010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907128"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..724
                     /locus_tag="VGC82_10470"
     CDS             299..724
                     /locus_tag="VGC82_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423735.1"
                     /translation="MGIGSITLWRSYTGTVPETDRVVATRQLQARTAQASEQLVEKTK
                     GLEATQQESIDQLQVVQDQLTTVRRLLAAQQADTRKLSEQVGTLTESIDSLRHSFAST
                     QSSEPATPPSTRTRSARGSGKTKPHGIKSANRKRGKTQG"
     gene            1245..2084
                     /locus_tag="VGC82_10475"
     CDS             1245..2084
                     /locus_tag="VGC82_10475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0423736.1"
                     /translation="MAPLSRRGFMTASAAVTAGYTLAAGPVRADVIKTDTDGLTTGDA
                     KIKVADGEMPGYFAKPNGVSNPPVVLVAMEIFGLHEYIKDVTRRLAKLGALAVAPDYY
                     FRKGTDLTNITDIPQLLPIVNAKPDAELLSDLDSTVAWAKSQGGDTSKLGIIGFCRGG
                     RTVWEYAAHSPALKAGAAFYGPPVDPPNPLWPKSPTQLAPEMKAPVIGLYGEADTGIP
                     VATVEALKAALAENKKTAEFKIYPGAPHGFHADYRASYRKEAADDAWANMQAWFKKYG
                     VLS"
     gene            2301..>2510
                     /locus_tag="VGC82_10480"
     CDS             2301..>2510
                     /locus_tag="VGC82_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423737.1"
                     /translation="MFNIPDAMQTAYQLRNANGLGVENTGAAVFIPQLSEPNPPHAQL
                     SRTRCDAEVLFAQLHPANAPAIAGHH"
BASE COUNT          523 a          819 c          742 g          426 t
ORIGIN      
        1 cgtgccaacc tttggtgacc agcggagtcg tgcagccaaa cgacgctggc ggttggtcca
       61 atagattcgc tccgtcgcgg aaaagcctga tttgagtgag gttttcgtcc gagataccgt
      121 aaacaaccgg taaatcccgg gttttagcgc tgcacagcct gtggtaatca agggtattgc
      181 aaatcacctg cggctttgga gtctcatgac cgaatttgcc tcccggaata caagttcgct
      241 tggacgcatc ggcggacagc cgatcgccgt cggtgcagtc gccctcgcca tcgttctgtt
      301 gggcataggc tcgatcacgc tctggcgcag ctataccggc accgtgcccg agaccgaccg
      361 ggtggtcgca acccggcaat tgcaggcccg caccgcgcaa gcgtccgagc aactggtcga
      421 aaagaccaag ggcctggaag cgacccagca ggaatcgatc gaccagttgc aggtggtgca
      481 ggatcagttg acgaccgtgc gtcgcctgct cgccgcgcaa caggccgaca cgcgaaaact
      541 ttccgaacag gtcgggaccc tgaccgaatc aatcgacagc ctgcggcatt cctttgccag
      601 tacgcagtca tccgaaccgg ccacgccgcc gtcgacccgc acgcgatcgg cccggggatc
      661 cggcaagacg aagccgcacg gaatcaagag cgccaatcgc aagcgcggca aaacgcaggg
      721 ctgacggcat aggccgccgg aaacgtccgg gtcagctcac gaccagaaca agcccgcgcg
      781 agcggattca gaatactccc gcaccgccaa cacacccttc gggttggtcg cgggtatctg
      841 gccgcccgct caaacccggc cggtcgggct ctgattggcc cacagaaccg ccgacgtcac
      901 gtttccggcc gcgctacggc aaaaaggtgc ccgccggaac tttttcccgc cgagtgtgaa
      961 ctggttcaca tatgcccgtg cgaatccggg gcatcctaca acctaccgga tggcgtgagc
     1021 cgatttcaat gtccggggct ggcaaggtcg ttgacggtat actgcgtcga cggccttgtt
     1081 ttttgcgccc tgttttccgg tcctgcgtcg gcgacccgac caatctcgca agcaggtgat
     1141 ttgcgttcag cccggtttgc cgcctgaatg cgcctcctga aaatggagga aattcaatga
     1201 gtccagaacc gctgctgacc tcagatgtca tcggactgac aaaagtggcg ccgttgtcgc
     1261 gccgcggctt tatgaccgcg tccgcggctg tgacggccgg ctatacgctg gccgccgggc
     1321 cggtgcgagc cgatgtgatc aagaccgaca ccgatggcct caccacgggc gacgccaaaa
     1381 tcaaggtcgc cgacggcgag atgcccggct atttcgccaa acccaacggc gtcagcaacc
     1441 cgccggtggt gctggtggcg atggagattt tcggcctgca cgaatacatc aaggacgtga
     1501 cgcggcgcct cgccaagctc ggcgcgctcg cggtggcgcc ggattactat ttccgcaagg
     1561 gcaccgacct caccaatatc acggacatcc cgcaattgct gccgatcgtg aatgcgaagc
     1621 cggatgccga actgctgtcc gatctcgaca gcacggtggc ctgggccaaa tcgcagggcg
     1681 gcgatacctc caagctcggc atcatcggct tctgccgcgg cgggcggacc gtctgggaat
     1741 atgccgccca cagtccggcg ctgaaggcgg gcgcggcttt ctacggcccg ccggtcgatc
     1801 cgccgaaccc gctctggccg aagagcccga cgcagcttgc gcccgagatg aaggccccgg
     1861 tgatcgggct ctacggcgaa gccgacaccg gcattcccgt cgccaccgtc gaagcgttga
     1921 aagcagcact cgccgagaac aagaagaccg ccgagttcaa gatctatccc ggcgcgccgc
     1981 acggcttcca cgccgactat cgcgccagct accgcaagga agccgccgac gacgcgtggg
     2041 ccaacatgca ggcctggttc aagaaatacg gcgtgctgag ctaatccggc tggctaatcg
     2101 acagacgaca aaacggcgcg cagccatcgg ctgcgcgcta tttgtctgac gcggcctatc
     2161 tgtcgcgatc gcgcctgcga ctggccttgg acaaaacgtg ccttgacgtc tgttcgaatt
     2221 cccagagcac ccacacgaac gccgtgccca aactgacgac gataccgagg aagacaaacg
     2281 tcaaagccgg ggttgtgagc atgttcaaca tccctgatgc gatgcaaacg gcgtaccagc
     2341 tacgcaacgc gaacggcctc ggtgttgaaa acaccggggc cgcagttttc atccctcaac
     2401 tatccgaacc gaatccgcct cacgctcaac tcagcagaac ccgttgtgac gcagaggttt
     2461 tattcgcaca gctgcacccg gcgaatgcgc cagccatagc gggtcatcac
//
LOCUS       DASWQN010000617         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2434240, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000617 DASWQN010000000
VERSION     DASWQN010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2434240"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VGC82_10485"
     CDS             complement(<1..633)
                     /locus_tag="VGC82_10485"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0423738.1"
                     /translation="MKAVICQQFGGPEVLVVREVPDPPSPGTDEIQVKIAARGVQYVD
                     VLMLAGKYQFRPEPPFIPGSEAAGHVVAVGAGVTAFAVGDTVMSRHSLGALAELGNAK
                     AVLCDKVPAGLTLSQAGVFRGAYTTAYHALLQRGRMQAGEWVLVHGAAGGIGIAAIQV
                     AKVFGAKVIATAGSEEKRQACLEEGADHAIDYRSGFVDKVKELTGGRGVDI"
     gene            783..1523
                     /locus_tag="VGC82_10490"
     CDS             783..1523
                     /locus_tag="VGC82_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0423739.1"
                     /translation="MSSSATLTGPVVASNGDEEQILEALRGTPLLRAVSPQVMDRLVK
                     ISEIVNVAEDTELYRQGEAAGALYIVLDGQLAGFSTAANGTTAVVEVIRTGETLGLAT
                     LLARLPRLMGVRTVTASRLLAVEADGLLELVEQEPSLVTALLRAEANEFHALVRQVCD
                     LKLRTTAQRLGCYLLSLSTERQANATAMRLPFDKRLLAARLGCRQENLSRAFAALRGF
                     GVETHGARVILHDIAKLRDYSEPDEALA"
     gene            complement(1645..2898)
                     /locus_tag="VGC82_10495"
     CDS             complement(1645..2898)
                     /locus_tag="VGC82_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423740.1"
                     /translation="MTLTVVPEDDGSGHWKRNLWVCVFGSFTTIVAMTLLLPFLPLYV
                     EQLGVTDHAAIVQWSGAAYGATFLSAALFAPLWGRLGDQYGRKLMLIRASLGMAVAMS
                     LIGLSQNVYQLVALRLLAGLLGGYSSGSMVLVATQTPRQRTAWALGMMSSGVMAGNLA
                     GPLIGGVLPPLIGIRMTFLAAGGIIFVAFLATTFLIKEEPRPRRSAAARKLGGWAAVA
                     DKRPVVAMLATGMLLMFANMSIEPIITVYVAQLVPDPAHVTIVAGLVMSAAALGSILS
                     ASRLGKLADRVGHWTVIVFCLLVCALLLIPQAFVTAGWQLIVLRFLMGLSLGGLLPCV
                     ATVIRHSVPERSAGGILGYSTSSQYVGQVTGPLIGGFIGGHFGMRAVFLGTSVLMAAG
                     AFYAWISRPRRPSGGRESASGSSYD"
BASE COUNT          592 a         1001 c          896 g          495 t
ORIGIN      
        1 aatgtccacg ccccttccac cggtcagttc cttcacctta tcgacgaacc cggagcggta
       61 gtcgatcgca tgatccgcac cttcctcaag acaggcctgg cgcttctcct cgctgccggc
      121 ggtggcaatg accttggcgc cgaacacctt cgccacctgg atcgcggcga tgccgatacc
      181 gccggccgcg ccatgcacca gtacccactc gcctgcctgc atccgcccgc gttgcagtaa
      241 ggcgtgatag gccgtggtgt aggcgccgcg aaaaacccct gcctgggaca aggtcaagcc
      301 tgcgggcacc ttgtcgcaca ggactgcttt ggcgttgccg agttccgcga gggcacccag
      361 cgagtgccgc gacatcactg tatcgcccac cgcgaacgca gtgacgcccg cgccgacagc
      421 gacgacatga cccgcggcct cgctgccggg aatgaacggt ggctcaggcc ggaactggta
      481 ttttcccgca agcatcaaca cgtcaacgta ttgaacgcct cgtgccgcga ttttaacctg
      541 gatctcatcg gtgccggggg atggcggatc cggcacttca cgcaccacaa gcacctcagg
      601 gccgccaaac tgctgacata tcactgcttt cataacgaac cctttccgta tcggattaca
      661 aatcacgcaa ataacatctg tgagaccacg ccaacgacac ggaaccttga tcgtgatcaa
      721 ttccttccgg tagaaattcc ggtgacgaaa ttctgcgttc aattcacgac ggcgaggttt
      781 gtatgtccag ttccgctacg ttgaccggtc cggtcgtagc cagcaatggc gatgaggagc
      841 agatcctcga ggcgctgcgg ggaacgccgt tgctgcgtgc cgtctcgccc caggtcatgg
      901 accggctggt gaagatcagc gagatcgtga acgtcgccga agacaccgaa ctttaccgtc
      961 agggtgaagc cgcgggcgcc ttatatatcg tgctggacgg ccagcttgct ggattcagca
     1021 cggcggccaa cggcacgacc gcggtcgttg aagtgattcg taccggcgaa accttgggtt
     1081 tggcgacctt gctggcgcgc ttgccacggc tcatgggggt tcggaccgtg acggccagcc
     1141 ggctgctagc cgtcgaggcc gacggattgc ttgaactggt cgagcaggaa ccgtcactgg
     1201 tcaccgcgtt gctgcgggcc gaggccaacg agtttcacgc cctggtccgc caggtgtgcg
     1261 acctgaagct tcgcaccacg gctcagcggc tcggctgcta cctgctttcc ctatcgacgg
     1321 agcgccaggc caacgcgacc gccatgcggc tgccgttcga caagcgcctg ctggccgctc
     1381 ggcttggttg ccgccaggaa aacctttcgc gtgccttcgc cgcactccgc ggattcgggg
     1441 tcgagaccca tggggcgcgc gtcattctgc acgacatcgc aaagttgcgg gactattcgg
     1501 agccggacga ggcactcgca taatctcccg cctataggtg tccgcctgca gcttaccgcg
     1561 tcgggtcgag cgcgccccag tagatccgcc ttgtgggcct tcgcccgcca agacgagggg
     1621 cggaccctgc accagtcgga gcagttaatc ataactggac cctgaggcgg actctctacc
     1681 gccgctcggc cgccgcgggc gcgatatcca ggcatagaac gccccggcgg ccatcagtac
     1741 cgatgtgccc aggaacacgg cgcgcatgcc gaaatggccg ccgatgaagc cgccgatcag
     1801 tggaccggtg acctgcccga catattgcga tgaggtcgag tatccaagga tgccgccggc
     1861 gctccgctcc gggacgctat ggcgaatcac cgtcgcaacg catggcagca agccaccgag
     1921 cgagagtccc atcaggaagc ggagcacgat caactgccat ccggcggtga cgaacgcctg
     1981 cgggatcagc agcaaggcgc acaccagcag gcagaagacg atcaccgtcc aatggccgac
     2041 gcgatccgcg agtttgccca gccgggaagc cgaaagaata ctgcctagtg ccgcggcgga
     2101 catcacgagc cccgcgacga tggtgacatg agccggatcg ggaacgagct gggcgacata
     2161 gaccgtgatg atcggttcga tcgacatgtt cgcgaacatc agcaacatcc cggttgccag
     2221 cattgcgacg accgggcgct tgtcggccac cgcggcccag ccgcctagct ttcgcgccgc
     2281 cgccgatcgc ctcggacggg gttcctcctt gatcagaaac gttgtcgcca ggaatgcaac
     2341 gaaaatgatc cccccggccg cgaggaatgt catgcggatg ccgatcaacg gcggcagtac
     2401 ccctccgatc agcggccctg ccaggtttcc cgccatcacg cccgaggaca tcatgccaag
     2461 cgcccaggcc gtccgctgcc gcggtgtctg cgttgccacc agcaccatcg agccggacga
     2521 atagcccccc agcagacccg ccagcagccg cagcgcgaca agctgataca cgttctgcga
     2581 cagcccgatc agcgacatcg ccacagccat cccgagactc gcccgtatca gcatcagctt
     2641 gcggccgtac tggtccccca accggcccca cagcggcgcg aacaatgccg cggacaggaa
     2701 cgtcgcgccg taggcggcgc ccgaccactg cacgatggcg gcgtgatcgg tgacgccgag
     2761 ttgctcgaca tacagcggca gaaacggcag gagcagcgtc atcgcgacga tcgtggtgaa
     2821 cgaaccgaag acgcagaccc aaagatttcg tttccagtgt cccgacccgt cgtcctcagg
     2881 gaccacagtc agggtcacag tgcgggaatc acccggtcaa caaacagatt caggctcgcc
     2941 gcggtctgct cgtagctcat tgtgccgagg cggaactgca tgat
//
LOCUS       DASWQN010000618         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2567096, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000618 DASWQN010000000
VERSION     DASWQN010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2567096"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..715
                     /locus_tag="VGC82_10500"
     CDS             74..715
                     /locus_tag="VGC82_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423741.1"
                     /translation="MTRTEILDRYRHLRAISTQHHNAALAFVSRPTILEHAKRLGLAA
                     GKALIADNHETMTLLFDLNLYTAKEGRSRALDRYARAAKLPPGSDEAHMLDAMRHPNF
                     SIWRLERKHEAAGLVVTDLLREREEWLVDENLERSAPNDAVFAARVCKPESFAMTCGV
                     VVPLTSELIEKAMHSALAGKAEKPEQLALDPRFATALYRAAIDSGIMNHVAYE"
     gene            900..1805
                     /locus_tag="VGC82_10505"
     CDS             900..1805
                     /locus_tag="VGC82_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0423742.1"
                     /translation="MLDGKVAVVTGAGGGIGRATAIALAANGARVVVNDIGATVTGEG
                     QDAGPAQRVVQEIEQANGAGRAVANSGSVASWEDAQRMVQDAIDNFGRIDIVVNNAGI
                     LRDRMFHYMSIEEWDAVIKVHLYGAFYVSRAAVPHFRKQESGCYTHITSTSGLIGSVG
                     QTNYGAAKLGIAGLSRCIAMDMQRYHIRSNCIGPHAFSRMIETVPGQTEEQLQARAAK
                     TRPDHIAQLIAFLGTDAAADVSGQIFGVRGNEVYLYNQPRPIRIMARTDGWTPEKLAA
                     EWLPAVRTSFTPLERTRDVYPWDPV"
     gene            1966..>2444
                     /locus_tag="VGC82_10510"
     CDS             1966..>2444
                     /locus_tag="VGC82_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0423743.1"
                     /translation="MPSTLRGADIVARTLERAGHTDIFTLSGNHIMSLFDAAIETKLA
                     LIHVRHEAATVHMADAYGRLTGRPGIAWVTGGPGHANAVGALFTALGQETPMVLFSGH
                     TETDQLGRGGFQELRQVDMAAPVCKAAWMATDTATVGMEVAKAIRLATSGRPGPVHL"
BASE COUNT          469 a          802 c          777 g          396 t
ORIGIN      
        1 gcaggtctat cctgccggga caacccacaa cgccgccgca cgtcgttcgg cggaagaaag
       61 gcgaggatcc ggcatgactc gaacggaaat cctggaccgc taccgtcatc tgcgcgcgat
      121 cagcacgcaa caccacaacg ccgcccttgc cttcgtatct cgcccgacaa tcctcgagca
      181 cgccaagcgg ctcggactgg cggcagggaa ggcactgatc gccgacaacc acgagacgat
      241 gacgcttctc ttcgatctca acctttacac cgccaaggag ggccgctcgc gggctttgga
      301 ccgttatgcg cgagcggcaa agctcccacc cggctccgat gaggcgcaca tgctcgatgc
      361 catgcgccac cccaatttct cgatctggcg ccttgagcgg aaacacgagg cggctgggct
      421 tgtcgtcacc gatttactgc gtgaaaggga ggagtggctg gtggacgaaa acctggaacg
      481 gagcgcacct aacgacgcgg tgttcgccgc acgcgtttgc aagccggaaa gcttcgcgat
      541 gacctgtggc gtcgtcgtgc cgcttaccag tgagctgatt gagaaagcca tgcacagcgc
      601 ccttgccggg aaagcggaaa agccagaaca gctcgccctg gatccgcgct ttgccaccgc
      661 gctctaccgc gccgccatcg acagcggcat catgaaccac gtcgcctatg agtgatgccg
      721 tctgaagcac catcacgccg agcgaagccg cgatctggcg gcgaaagatg tcgaagcgct
      781 ggcatccgaa atggtttccc gaggctgttc cgaacgtcca catccggccg ggatgatgac
      841 agcgaccgcg ctctgtcgca ttgtgcggcg gttcagattg aaggaggaaa cagatgggca
      901 tgcttgatgg aaaggtcgcc gtggtcacgg gggccggcgg cggtatcgga cgggcgacag
      961 cgattgccct ggccgcgaac ggggcccggg tcgtggtcaa cgacatcggc gcgacggtta
     1021 ccggagaagg ccaggatgcc ggtccggccc agcgcgtggt gcaggagatc gagcaggcga
     1081 acggcgccgg ccgagcggtg gccaacagcg gcagcgttgc gagttgggaa gacgcccagc
     1141 gcatggtgca ggacgcgatc gacaatttcg gccggatcga catcgtggtg aacaatgccg
     1201 gtatcctgcg cgatcgcatg ttccattaca tgtcgatcga ggaatgggac gccgtcatca
     1261 aggtccatct ctacggcgca ttctatgtct cccgcgccgc cgtgccgcat ttccgcaagc
     1321 aggaaagcgg ctgttatacc catatcacgt ccacctccgg cctgatcggc agtgttggtc
     1381 agaccaacta cggcgccgcc aagctgggca tcgccgggtt gtcccgctgc atcgcgatgg
     1441 acatgcagcg ctaccacatc cgctccaact gcatcgggcc gcacgcgttc agccgcatga
     1501 tcgaaaccgt gccgggccag accgaggagc agttgcaggc gcgtgcggcg aagacccgtc
     1561 cggaccacat cgcacagttg atcgcctttc tcggcaccga tgccgcggca gacgtctccg
     1621 gtcagatttt cggcgtacgc ggcaacgagg tttatctcta caaccaaccg cggccgatcc
     1681 ggatcatggc ccgcacggat ggctggacgc cggagaagct ggcggcggaa tggctacccg
     1741 cggtgaggac cagcttcacg ccgctggagc gcacccgcga cgtctatccg tgggacccgg
     1801 tctgatagcc cgcgcgttga cttgatccgg gaagccgccg gcccacccgg gacctgactg
     1861 tcccgcccct gcgttcgccg cgtctgtggg gcgtgcgcgg gggaccagcc ggccgcggga
     1921 cgccggtgcg ggcggacaac atcccaagac caaggaccac cacgaatgcc atccactctg
     1981 cgcggtgccg acatcgttgc ccgaacgctt gaacgtgccg gtcataccga cattttcacg
     2041 ctgtccggca accacatcat gtcgctgttc gacgccgcga tcgagacgaa attggcgttg
     2101 attcacgtcc ggcatgaagc cgccaccgtg catatggccg atgcctacgg gcgcctgacc
     2161 ggacgacccg gcatcgcctg ggttaccggt ggaccgggcc acgccaacgc cgttggcgca
     2221 ttgttcaccg cactggggca ggaaacgccg atggtgctgt tttccggcca taccgaaacc
     2281 gaccaacttg gtcgcggcgg cttccaggaa ctgcggcagg tcgacatggc cgccccggtc
     2341 tgcaaggccg cctggatggc caccgacacc gccaccgtcg gcatggaggt tgcgaaggcc
     2401 attcgcctgg ccacatccgg tcgcccagga ccggtgcacc tgag
//
LOCUS       DASWQN010000619         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1379545, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000619 DASWQN010000000
VERSION     DASWQN010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379545"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..670)
                     /locus_tag="VGC82_10515"
     CDS             complement(377..670)
                     /locus_tag="VGC82_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423744.1"
                     /translation="MTDAQLRALLLDCLKLWEVKGKVVVRETYLVIETPCGLFTVTRA
                     GPDLRPVRWFYQTPERAAARRPARAAPSVVALLSALRNAMEGAGGDRLRVGGG"
     gene            complement(674..2101)
                     /locus_tag="VGC82_10520"
     CDS             complement(674..2101)
                     /locus_tag="VGC82_10520"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0423745.1"
                     /translation="MVDLVIRGDTVVTPQGVGAHDILIAGGTIAAVAAPGSIPIPEGA
                     RVIDARGKIVIPGGIDPHVHCKWHLPGPDGSASETQPPDVVSKAAVHGGTTTMIDFTR
                     ASQGKDVRDAIEKREMDWKGHCACDYAQHLMVEGALPVDLPAQIGEAIQSGFPTVKIF
                     TTDITPSRKGRMVDFGDIWEIFQVLAANQGLGVIHSEDNDIVMHMYGKLIREGRTGFE
                     NMAEVHNTLSEDVSFRRVIRLAEKVTGTALYMMHVSAATGVSAIHEARRRGVPIYGES
                     LHQYMLYSSEDYKRPNGQIYHTYPSLKGPEDHVALWQGTLDGSINCVATDEICCTLKQ
                     KLQGVRIDDTTGGNAGVEPRVALMYTEMVGKRHYSLQRYVDLISTNAAKIMGLYPRKG
                     ALAAGSDADICVLDPADQRVIRAEDLHEADYTPWEGRKMDAWPCLTVLRGKVVVENGV
                     FKGSLDDGKWQHRKVANEMFTAPRL"
     gene            complement(2086..>2265)
                     /locus_tag="VGC82_10525"
     CDS             complement(2086..>2265)
                     /locus_tag="VGC82_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423746.1"
                     /translation="RRHWRRTGRAGLVWRLRANQQDLIRGAVADASPRLAHPARLGDD
                     VPQGHDKRGKTWSIW"
BASE COUNT          413 a          780 c          632 g          440 t
ORIGIN      
        1 gcgcgatcaa ccagagaaaa tcctgccggt gatgaacggt cagcaaatcg tatcggcggt
       61 cgatcgactc gaatttccga tcgattgatt caaacttacg gtctatcgca tcaaaccgcc
      121 ggtccatgcg gtcaagcatc agatcctggc gctgctccag ccgctccagc gtggccgcag
      181 tcgtcttcgc gatatgttcg agaactgcga tgccagcttc cctaccttca tgatacggag
      241 cttgggcagg aaatcaaccg ggataccagg catcgcgtct tcctcaagaa cgcctgaact
      301 tacccccggc aaatattaac acagtataaa tcggtggcag agccccgtca cttgcacgca
      361 atagtatgcg tgacttctat ccacctccta ctcgcagccg gtctccgccc gcaccctcca
      421 tcgcattccg caacgccgac agcagcgcca ccaccgacgg ggcggctcgt gccggccgcc
      481 gtgccgccgc ccgctccggc gtctggtaga accagcgcac cggacgcaga tcggggccgg
      541 cgcgggttac cgtgaacaga ccgcacggcg tctcgatgac aaggtacgtc tcgcgcacca
      601 cgaccttgcc cttcacctcc cacagcttca gacagtccag cagcagtgcc cgaagctgcg
      661 cgtccgtcac cagctacagc cgaggggccg tgaacatctc gttcgccacc ttgcgatgct
      721 gccatttccc gtcatccagg ctgcctttga acacgccgtt ctccaccacc accttgccgc
      781 gcagcacggt caggcatggc caggcatcca tcttgcgccc ctcccacggc gtataatccg
      841 cttcgtgcag gtcctcggct cggatcaccc gttggtccgc ggggtccagc acgcagatgt
      901 cggcatcgga tcccgcggcc aatgcgcctt tgcggggata caggcccatg atcttcgcag
      961 cattggtgga aatcaggtca acgtaacgct gtaacgagta atgccgcttg ccgaccattt
     1021 cggtgtacat cagcgctacc cgaggctcga cgcccgcatt gccgccggtg gtgtcgtcga
     1081 tccgcacgcc ctgcagcttc tgcttcaggg tgcagcagat ctcgtcggtg gcgacacagt
     1141 tgatcgaccc gtccagcgtt ccctgccaca aggccacgtg gtcctccggc cccttcagcg
     1201 aaggataagt gtgatagatc tggccgttcg gccttttgta gtcctcgctg ctgtaaagca
     1261 tgtattgatg caaggactcg ccatagatcg gcacgccccg acgccgagct tcatggatcg
     1321 ccgaaacgcc ggtggcggcg gagacatgca tcatatacaa ggccgtgccg gtcacctttt
     1381 ccgccagccg gatcacccgc cggaacgaga cgtcctcgga caacgtgttg tgcacctcgg
     1441 ccatattctc gaacccggtg cggccttcgc ggatcagctt gccatacatg tgcatgacaa
     1501 tatcgttgtc ctcggagtgg atcaccccaa gtccctggtt cgccgccaga acctggaaaa
     1561 tctcccagat atcgccgaaa tcgaccatcc tgcccttgcg gctgggcgtg atgtcggtgg
     1621 taaagatctt taccgtggga aagccggact gaatcgcctc gccgatttgt gcgggcaggt
     1681 caaccggcag cgcgccctcg accatcaggt gctgcgcgta atcgcaggcg cagtggccct
     1741 tccagtccat ctcccgcttc tcgatcgcat cccgcacatc cttcccctgc gatgctcggg
     1801 tgaagtcgat catggtcgtc gtgccgccat gaaccgccgc cttgctgacc acgtccggag
     1861 gctgcgtttc actcgcgctg ccgtccgggc caggcaaatg ccacttgcag tggacatgcg
     1921 gatcgatgcc gccgggaatg acgatcttgc ccctggcatc gatcaccctt gcgccttccg
     1981 gaattgggat gctgccaggt gccgcgaccg cggcgatggt cccgccggcg atcaggatgt
     2041 catgcgcgcc aacgccctga ggcgtcacca ccgtgtcgcc acggatcacc agatcgacca
     2101 tgtcttcccc cgcttgtcgt gtccctgcgg cacatcgtcg cccagccgag ccggatgggc
     2161 aaggcggggg gacgcgtcgg ccacggcacc gcggattagg tcttgttgat ttgcgcgcaa
     2221 ccgccacacc agtcctgcgc gccctgttcg gcgccaatgc cgccg
//
LOCUS       DASWQN010000620         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2170990, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000620 DASWQN010000000
VERSION     DASWQN010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2170990"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="VGC82_10530"
     CDS             complement(<1..1416)
                     /locus_tag="VGC82_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY0423747.1"
                     /translation="MADAPLIVVGIGASAGGIEAFRQFFERMPADSGLAFVVVLHLAA
                     ARRSLLPEILARWTAMPVSEAHDGVAVAANHVLVIPSGNVAELRDGCLWLAPMAPGSP
                     RFLAPIDVFFDSMAASLAENAIGIILSGTGHDGALGLKAIRARGGLTLAQGGDGTQPG
                     YAGMPDSAVAAGAVDLHVPAEEMPGHIIAARQVRSATLREAEEPGPDGESIRLAICAI
                     LRSRLDHDFYQYKHQTFMRRVQRRMQVLRVARYDEYVKRLEADREQVVLLFRDLLISV
                     TSFFRDASVFTALEQAIIPRLFEGKDASSDLRIWVPGCATGEEAYSLAILLRAHMDTL
                     TTVPKVQIFASDIDEVAIATARAGRFPATLLNGMSAERRARYFVEGPGGYAVKQEVRE
                     LCTFSAHSLIRDPPFSRIDMVSCRNLLIYLDSNLQDRVIPIFHYALVPRGVLVLGLSE
                     SIARHDKYFQPLDRSNRIFVRR"
     gene            1889..>2572
                     /locus_tag="VGC82_10535"
     CDS             1889..>2572
                     /locus_tag="VGC82_10535"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0423748.1"
                     /translation="MPQINILNLTRLSAEDRARIEAVDPSVRLTDAAGWFDGEYRETW
                     PAFTAQRYLAPGTTGRGSRQDRDRLLAEADIVIGGWPFPLDLRARAARLKWFHQRPAG
                     ASNLLRGDLWRSDVTVTTSRGAANALAIAEYAMAGILHVAKGLHRAAVDRNGGGFSAR
                     AYRPLLLQGKTVCVVGAGGIGMQVGRLCAAIGMRVVGTRRHQAAELPEGFSEIGSAAD
                     LDRFLSVSDV"
BASE COUNT          518 a          795 c          820 g          439 t
ORIGIN      
        1 ccggcgaacg aaaatccggt ttgaccggtc cagtggctga aaatacttgt cgtgacgggc
       61 gattgactcc gacagtccga ggacgaggac accgcgaggc accagcgcat agtggaagat
      121 cggaatgaca cgatcctgca aattactgtc cagatagatc aacaagttgc ggcaggaaac
      181 catgtcgatg cgggagaatg gaggatcgcg gataaggctg tgggcggaaa acgtgcaaag
      241 ttcccgcacc tcctgcttga ccgcgtaacc tcccgggcct tcgacgaagt accgggcccg
      301 ccgttcggct gacatgccgt tcagcagcgt cgcgggaaaa cgtccggccc gggcggtggc
      361 gatcgccact tcgtcgatgt cgctggcgaa aatctggacc ttcgggaccg tcgtcaacgt
      421 gtccatgtgc gcgcgcagca gaatggccag ggaataagct tcttcccccg tggcgcaccc
      481 gggcacccag atccgtaggt cgctggatgc atccttgccc tcgaaaaggc gggggatgat
      541 cgcctgttcc aacgcggtga aaaccgaggc gtcgcggaaa aaactggtta cgctgatcag
      601 caggtcgcga aacagcagca cgacctgctc gcggtcggcc tcaagccgct taacgtattc
      661 atcgtaacgt gccacccgaa gaacctgcat ccggcgctgc acgcgccgca tgaatgtctg
      721 gtgtttatat tggtagaaat cgtgatcgag ccgggaccgc aggatcgcgc agatcgccag
      781 gcgaatactt tcgccatccg gtcccggctc ctctgcttcc ctcagcgtgg cggagcgaac
      841 ctgccgagcg gcgatgatat ggccgggcat ctcctcggcc ggtacgtgca gatcgacggc
      901 gcccgccgcc acggcgctgt ccggcatacc ggcatatccg ggctgggttc catcgccgcc
      961 ctgcgccaag gtcaggccgc cgcgggccct gatcgccttg agaccgaggg cgccatcgtg
     1021 acccgttccg gagagtatga tgccgatggc attctcagcc aggctggccg ccatcgaatc
     1081 gaaaaacacg tcgatcgggg ccagaaaccg gggcgaaccc ggcgccatcg gggccagcca
     1141 cagacagcca tcgcgcaact cggccacgtt gcccgacggg atgaccagga cgtggtttgc
     1201 cgcaacggcg acgccgtcat gcgcttcgct gacgggcatg gcggtccagc gcgccaggat
     1261 ctccggcagc aaactcctgc gggcggcggc gagatggagg acgacgacaa aggcaagacc
     1321 gctgtcggct ggcattcttt cgaagaactg gcgaaacgct tctatgccgc cggccgaggc
     1381 gccaattccg acgacgatga gcggtgcgtc agccatcgtc tcgtcccgcc gtcgccggcg
     1441 accggcccac ccgatcgtaa aaagaatcgc actgcaagac agcccgcata tggatgagtt
     1501 tacagcatgt tcccaaggtt gcgcgaggca gtttgctcgc tcggaccaag acgggcgcga
     1561 cgccgccttt gcaaggcctc atgcgcagca gtgaaggata aacgaagtgg aactgaacgc
     1621 caagactatg caacagaacg ccaaaacgac cccggaagcg agatttaacc gtacgcgaat
     1681 ttgttgccac agttgggtca ttacattact gagaatcaca tcgacgtgac tctagtatgc
     1741 gccgcaggag cctcgtggat ccagtcggcg cgacataatc gagcccgagg caaagcatcg
     1801 tgaaggacac gcgctgccgc ctcaacgcct tcggtttaac accgcggcga acctgcctta
     1861 aggttcgcgc tcgcgtcgga accggatcat gccgcaaatc aacatcctga accttacccg
     1921 tcttagcgcc gaagatcgcg ccagaatcga ggcggtcgat ccatcggttc gactgaccga
     1981 tgccgccggc tggttcgatg gcgaataccg cgaaacttgg cccgcgttta ccgcgcagcg
     2041 gtatctggca ccgggcacca cgggccgggg aagccggcaa gaccgcgacc gcctgctggc
     2101 cgaagccgac atcgttattg gtggttggcc gtttcctcta gacttgcggg ccagggctgc
     2161 gcggctgaaa tggtttcatc agcgcccggc gggcgcgagc aacctgctgc gcggcgacct
     2221 gtggcgaagc gatgtgaccg taacgacatc gcgcggcgca gccaatgcgc tggccatcgc
     2281 ggagtatgcg atggctggca tcctgcatgt cgccaagggt ctgcaccgag cggcggtgga
     2341 tcggaacggc ggcgggttca gtgcgcgcgc ctaccggccg ctgctgctcc agggcaagac
     2401 cgtctgcgtc gtcggcgccg gcggcatcgg tatgcaggtc gggcggttat gtgccgcgat
     2461 cgggatgcgg gttgtcggca cgcgccggca ccaggcggcg gaattgcccg aaggattcag
     2521 tgaaatcgga tctgccgctg atcttgatcg tttcttatcc gttagcgacg tt
//
LOCUS       DASWQN010000621         4352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_323891, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000621 DASWQN010000000
VERSION     DASWQN010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323891"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VGC82_10540"
     CDS             <1..1123
                     /locus_tag="VGC82_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0423749.1"
                     /translation="WKSPFGRWFLTGYEDTVLLLRDRRFGKGYDDPEALVRRFGPTAM
                     QEPAVVELSHMMLMRDPPDHTRLRGLVTKAFTARRIEALRVRIQDLTDRLLDKVVANG
                     RMDAIRDLAFPLPVLVICELLGIPEPDRDHFVTRTSSGGALLNPTPPTRAELDAANAG
                     SSRSGAYFEALFEQRRQEPQDDLITQLVQAEEAGDRLSTAELRANVNLLFAAGHETTV
                     NLIGNGILSLLRHPAQWQMLRDDPLLIPNAIEEILRFESPVQAVGRTVAEPFELRGVA
                     LAQDDIVVSLVGAANRDPAVFENPDRLDITRKDLRPLSFGGGIHFCLGAQLARIEAAV
                     VFETLARRLPSLRLVEPVQPKWRSSFTLRGLTELAVEWG"
     gene            complement(1274..1957)
                     /locus_tag="VGC82_10545"
     CDS             complement(1274..1957)
                     /locus_tag="VGC82_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423750.1"
                     /translation="MQVMIGMLVTFAALVLGLLTASVENTYDRAALDRQAYALQLTDL
                     DQCLRNIGPGGEHSRGLLKSYTAAVIASTWPLEPRPVGVDYPDTSTMPQVGSSPVLAS
                     LMNRIDLELRHMQPADSFQASVRQDCLATFHDVARARLLVIESVSRRISVPFQGILVF
                     WLMIVFATFGLAAPRNSLSLIGIILCAVSLSSALFVIADLGQPYGGLFAISSADMRTA
                     LAQMMASGS"
     gene            2352..2726
                     /locus_tag="VGC82_10550"
     CDS             2352..2726
                     /locus_tag="VGC82_10550"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY0423751.1"
                     /translation="MTLDRPPFRVLRPVAWGCVVLIAGLSLLPAEEMVRTSLGGHMEH
                     ATAYAGTALLIGLSYPAHRWKRIAAGLVAYAGVLELLQNFSPGRHPAILDWFSSSTGA
                     VIGLAFVHMADEVWRRKSRLSH"
     gene            complement(3047..3967)
                     /locus_tag="VGC82_10555"
     CDS             complement(3047..3967)
                     /locus_tag="VGC82_10555"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY0423752.1"
                     /translation="MLKDALYGGINAAVLTAMNADWSIDLDRMAGHCRWLLANGCNGL
                     AVLGTTGEANSLGISERIAVLEGLVERGIPAAKMLPGTGTTAIPDTVLLTRRAADLGC
                     RGALLLPPFYYKNPSDDGLLVYFNEVIQRVGGDIKLYLYNFPQQSAIPFSVDFIGRLL
                     KANPGKIKGIKDSSGNYENSLSYVEAFARDGFEVYAGDDSLLKPLLEKGSAGSITAAS
                     NVNCALGAEVYANWDNAKGAAAHEILAATRRAVISVPLIPALKSLVARNTGDQSWLNI
                     RPPHLKLTEAQQATLFNLFDASGITLAKAA"
     gene            complement(4036..>4352)
                     /locus_tag="VGC82_10560"
     CDS             complement(4036..>4352)
                     /locus_tag="VGC82_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HEY0423753.1"
                     /translation="LDLDSCRCSDYARRQRRVPDCVSLTPKALADIDWLPPSCGYRRI
                     RDGKGLAWWHPLVSGDPDTVHQAGVSVRGRAVSERRAGALEEHVVAWPGRPPKGRRPV
                     DS"
BASE COUNT          822 a         1365 c         1335 g          830 t
ORIGIN      
        1 ttggaaatcg ccgttcggcc gctggttcct gaccggttac gaggatactg tcctgctgct
       61 ccgcgaccgc cgcttcggca aggggtacga cgatcccgaa gccctggtcc gccgctttgg
      121 accgaccgct atgcaggaac ccgcggtggt cgagctgtcg cacatgatgc tgatgcgcga
      181 tccgcccgat cacacccgat tgcgcggcct tgtcaccaag gccttcaccg cgcggcggat
      241 cgaggcgctg cgcgtccgca tccaggacct gaccgaccgc ctgctggata aggtcgttgc
      301 aaacggccgc atggatgcga tccgcgacct ggcctttccc ctgccggtac tcgtcatctg
      361 cgaactgctt ggtattcccg aaccggatcg ggatcatttt gtcacgcgga ccagttccgg
      421 cggcgcgctg ctcaatccga cgccgcccac ccgagcggaa ctcgacgccg cgaatgccgg
      481 tagcagcaga tccggtgcat atttcgaggc gctgttcgaa cagcgccgcc aggaaccgca
      541 ggacgacctg atcacccaac tggtgcaagc cgaagaagca ggcgaccggt tgagcaccgc
      601 ggaactgcgc gccaacgtca acctgctgtt cgccgccggc cacgagacga ccgttaacct
      661 gatcggcaac gggattctgt cgctgctgcg tcatcccgcg cagtggcaaa tgctgcgtga
      721 cgatccgtta ttgatcccga acgcgatcga ggaaatcctg cgcttcgagt cgcccgtcca
      781 ggcggtcggc cgcacggtgg ccgaaccgtt cgaactgcgc ggcgtggctc tggcacagga
      841 cgatatcgtg gtgtcgctgg tcggggcggc gaaccgcgat ccggcggtgt tcgaaaatcc
      901 cgaccggctg gacattactc gcaaggattt acggccgcta tcgtttggcg gcggcattca
      961 cttctgcctc ggcgcgcagc ttgcccggat cgaggcggcg gtggtgttcg aaacgctggc
     1021 caggcggctg ccttcgctac ggctggtgga accggttcag ccaaagtggc gatctagctt
     1081 cacgttgcgc ggcttgacgg agctggcggt ggagtggggt tgataccgca tcgtcgccgg
     1141 cggtgttgcc acgtcgtgat cggcgcagag cctgttccgg tccatgctac aattccgtat
     1201 cattccgcga ccgcctggat ggcagaaccg gcgctctttg cctgttgaac cctcgcgagc
     1261 gctttcgggc gcatcacgag ccagaagcca tcatttgcgc gagggcagta cgcatgtcgg
     1321 cacttgagat ggcaaacagg ccgccatagg gctggccgag atcggcaatc acgaaaagcg
     1381 cactgctcaa tgagaccgca caaagaataa tcccgatcag ggaaaggctg ttgcgtgggg
     1441 ccgccaggcc gaatgtggcg aagacgatca ttagccagaa taccagaatg ccctgaaacg
     1501 gcacggagat tctccggctt accgactcga tcacaaggag gcgggccctg gccacgtcgt
     1561 ggaaagtcgc gagacagtct tgccgcacac tggcctgaaa cgagtccgcc ggttgcatat
     1621 gccgtagctc caggtcaata cggttcatca ggctggccag aaccggactg gaaccaacct
     1681 gtggcatggt ggacgtgtcc gggtagtcga caccgaccgg acgcggctcc aagggccatg
     1741 tactggcgat caccgcagcg gtatagctct tgagaaggcc tctggagtgt tcgccgccgg
     1801 gcccgatgtt gcggaggcat tgatcgaggt cggtgagttg cagtgcataa gcttggcgat
     1861 cgagagccgc acggtcataa gtgttttcaa ccgacgcagt gagaagtccg agcaccagcg
     1921 ccgcgaacgt caccagcatc ccgatcatca cctgcatggt ctgaaccgtt tcagctgccc
     1981 gatgcgtctc cgggagccgt ggccgaacgt agcggccaag gcctgcgctg gcgcaaagca
     2041 ccacgaatac ggcaaaagcc gaccagactt ccgacaccta acaccggccc cttgtttggc
     2101 gattcagctc tcagatagca ggggactggc taacgaccta cccgttcaac agcagcacgc
     2161 cggggcggta tccaccgcca acgccgatcc ctcggtatag gaaccgcgat gcggcgctgg
     2221 cctgccaatg gctggcgagc cggagcatcc gggcgcttct gcgacgcatg gccgtaattc
     2281 gattgaaaac cgaggggtgg tcattatatc gcgggcatcg aaggtggagc gaagcggctg
     2341 gtggagttga cgtgacgctt gatcggccgc cttttcgcgt tcttcgcccc gtagcatggg
     2401 gctgcgtcgt gttgatcgca ggcctgtccc tgttgccagc cgaggaaatg gtccgcacca
     2461 gtctgggcgg ccatatggag catgcaaccg cctatgccgg tacggcactg ctgatcggat
     2521 tgagttatcc ggctcatagg tggaaaagaa tagcggccgg gttggtggct tatgcgggtg
     2581 tcctcgagct cttgcagaat ttttcgccgg gccgacatcc agcgatactc gattggttct
     2641 caagctctac gggcgccgtc atagggctcg cttttgtcca tatggcggat gaagtatggc
     2701 ggcgcaaaag tcgattatcc cattgagagt ccgatgacga atagccgttt agagccgggc
     2761 gatccggctc agcggtatcg tggtcagcat cacatcaaga gcgtcgggcc aatcgccata
     2821 gcagggtttt ctccgcttgg gcataaccgg ccttgtgccg gtcacccaag actttgcctg
     2881 ttgagacaaa gtcttgggtg acagcgccgc gcccggcctt cacactatta tggaatgcta
     2941 cagcccgtgt ttgcccgggg tggagttttc atcctgatcg aacaccgaat gctgggtaaa
     3001 cctcaaacga tcaaggtcta aatgtccgca acgggtcccc gcatcatcag gcggccttgg
     3061 ccagcgtgat cccgctggca tcgaacaggt tgaacagagt cgcctgctgc gcctcggtca
     3121 gcttcaggtg tggcggacgg atattcagcc agctttggtc accggtattg cgtgccacca
     3181 gcgatttcaa tgccgggatc agcggcaccg agatcaccgc cctacgcgtt gccgccaaaa
     3241 tctcatgcgc cgctgcgccc ttggcgttgt cccagttggc gtacacctcg gcgccaagcg
     3301 cgcagttgac gttggatgcg gccgtgatgc ttcccgcgct gcctttttcc agcaacggct
     3361 tcagcagcga atcgtcgccc gcatagactt cgaacccgtc gcgggcaaag gcttcgacgt
     3421 aggataggct gttctcgtag ttgccgctgc tgtccttgat ccccttgatc ttgcctgggt
     3481 tggccttcaa cagccggccg atgaaatcca ccgagaacgg gatcgccgac tgctgcggga
     3541 aattgtagag atacagcttg atgtcgccgc cgacgcgctg gatcacctcg ttgaagtaaa
     3601 ccagcagccc gtcgtccgac gggttcttgt aatagaacgg cggcagcagc agcgcgccgc
     3661 gacaacccag atccgccgcc cggcgggtca acagcacggt atcggggatc gctgtcgtgc
     3721 cggtgccggg cagcatcttg gccgccggaa tgccgcgctc gaccaagccc tccagcactg
     3781 cgatgcgttc gctgatgcca aggctgttag cctcaccggt tgtccccagc acggcaaggc
     3841 cgttgcaccc gttggccagc agccagcggc agtgcccggc catccgatcg aggtcgatcg
     3901 accaatcggc gttcatcgcg gtcagcaccg ccgcgttgat tccgccatac aacgcatctt
     3961 tcaacatgtc gcagtctcct tcaggtacaa atccggcatc ttcagttaca acccagcatc
     4021 accggtccgg atcgctcaac tgtccaccgg ccttctgcct ttgggcggac ggccaggcca
     4081 cgccacgacg tgttcctcca gcgcccctgc ccggcgttcg ctgaccgctc gaccgcgaac
     4141 cgagaccccg gcttgatgca cggtatccgg atcgccggac accaaagggt gccaccaagc
     4201 caggcccttg ccatcccgta tccggcgata gccgcaggaa ggcggcagcc aatcgatgtc
     4261 agccagcgcc ttgggcgtca ggctgacgca atccggcacc cttcgctgac ggcgtgcgta
     4321 atcgctgcat cggcaggaat ccaggtcgag ca
//
LOCUS       DASWQN010000622         6958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1114688, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000622 DASWQN010000000
VERSION     DASWQN010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114688"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..1447
                     /locus_tag="VGC82_10565"
     CDS             416..1447
                     /locus_tag="VGC82_10565"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0423754.1"
                     /translation="MTSQVVAPSVLAATPPGMPPGTPLGTNDLNTKTQIKHVIIIYGE
                     NRSFDHVFGTYRSPSGDTVSNILSKGIVNEDGTPGPNFSKAAQFQAKTTDTYSVSPGK
                     TSVYQTLPPPFATIQAAENADYGLLPRDLRLLTTGATGLKPGSVDTRIANANALPSGP
                     YQLTPGQPYDAYAASPVHRYYQARQQADCDASKASEADPSGCANDLYPWVEVTIGAGS
                     NGKPQPSGFNDATTGEGSTAMGFYNVQQGDMAYFKKLADEYTISDNYHQPGMGGTGLN
                     SIPPVSPTASGTPTERAAPPHRQPTRSRTQIPSPARTTGTPRMATRAGRTVPAPMPAS
                     PASVRSSAT"
     gene            1501..2286
                     /locus_tag="VGC82_10570"
     CDS             1501..2286
                     /locus_tag="VGC82_10570"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0423755.1"
                     /translation="MNNYNPGYFGNGTVDTTDTFTIPPVPTPSIGNVLLAAGVSFRWY
                     GEGFTQYAADPADPTNVYCNICNPFQYQTSIMANETVRDQVLKDTTEFYTDLQAGVLP
                     AVSFVKPGALNDGHPASSKFSIYEAFVRKIITELRKQPKLWQSTAVFITVDEAGGYYD
                     SGYIQPVDFFGDGPRIPLIVVSPFTRGGRVSHEYGDHVSILKFIEANWGLPTISGRSR
                     DNLPNPVQSGANPYVPTNGPSLGDLMSMFQRSNDDRHDDANDD"
     gene            2646..5006
                     /gene="gspD"
                     /locus_tag="VGC82_10575"
     CDS             2646..5006
                     /gene="gspD"
                     /locus_tag="VGC82_10575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEY0423756.1"
                     /translation="MRGNIVRRQRSLALVLGLVQLAGCNHPSPPAIPALSPLPNGVTA
                     APARVDGGVGAPDALPPAQVAYGTNAAIPPAGSPANAEPGDVSLDFADTDIREVAAQI
                     LGTILKVNYTIDPSIHGTATLHTVRPLNRSELVPTLESLLAQNGAAVVTNGSLYRVVP
                     IGQAIVTAASGAGTAGAVVVPLRYATSEDLAKILQPYVGEGGKIAADPGRNALLISGE
                     PAAREGLIGLVRAFDVDVLARQSYALLPVNNGDVKDFTSALQDAFRGQAGGALAGLVR
                     VVPLARISAVLVVSSQQRYIEEVRRVYTLIERARRQTIRSWHVYYLQNSHSQDVAYVL
                     QQAFTPGNVTAQPSSNTRSQTGQSGTGQSGLGGSGGAIGGGGGGGGLGSGSAGSLGRS
                     GLSGGISGGGGGFGGGGGGFGGGLGQGGGQGGGQQAIGGPPQQAQQSVAGGANPLLGG
                     LEPGGSDQGTDTLRVIPNDQNNAILVFGTEREQNTMEAMLRKIDILPLQVRIDAVIAE
                     VTLNDTLEYGTQFFFKSGGINGILNTAAQTPVNAAGTALNLNFPGFFLGGSSRGGAPF
                     AIQALQQVTTVHVLSSPELLVLDNQPARLQVGSVVPYLSQSSQSTISPNAPVVSSINY
                     QQTGVIMEVTPRVNSGGLVTLDVMQDVSSVATGITTPGINSPTFNERNVTSRVVVQDG
                     QTIGLAGLITDSASLGNQGIPWLKDVPILGLLAGTQNNTRQRTELLILITPHVIHDQR
                     DARALTDDLRDQLINAAAVPGMLNGLRPSGQSDPSARLRKQLDLQQ"
     gene            complement(5040..5456)
                     /gene="gspG"
                     /locus_tag="VGC82_10580"
     CDS             complement(5040..5456)
                     /gene="gspG"
                     /locus_tag="VGC82_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEY0423757.1"
                     /translation="MSNRPRRDDRGFTLLELLVVIAILGLLIGLVAPAALRQLGSART
                     SVAKQSIERISSVLDLYKLDIGSYPSSDQGLRALIEPPSGISNWNGPYVKGGMPVDPW
                     NHPYVYRGPSARAGHDFDLCSQGPLGTGGDQEICNQ"
     gene            complement(5453..5899)
                     /locus_tag="VGC82_10585"
     CDS             complement(5453..5899)
                     /locus_tag="VGC82_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0423758.1"
                     /translation="MSIPFLACMAAAAAFYHLPPRVLPSIQAVEGGRPGLVQPNANGS
                     ADLGLMQINTIWIEPLAAYAHMTQQATIDRLIDDPCFNIAASAAIMRIYLTEARGDLM
                     TAIGFYHSHTPALGSAYQLKVLSSAGALFSRQPNGKRQDNNRLVNR"
     gene            complement(5919..>6958)
                     /gene="rfaE2"
                     /locus_tag="VGC82_10590"
     CDS             complement(5919..>6958)
                     /gene="rfaE2"
                     /locus_tag="VGC82_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEY0423759.1"
                     /translation="AVEQQLDAADVIVLSDYAKGVLCDGVLDAILARGNEKGRLIIAD
                     PKRADFAAYRGATVLTPNEHEVRVATRIDSEHDAEADRAGRMALEATGGRAVVVTRSA
                     KGLTLVCRDADALHLATRAREVADVSGAGDTLVAALAVALGAGASLPDAAMLANATAG
                     ISVGKQGTATVSRQELLDVLHLHELIETDRKVANLEDAVERVAEWRRRGLKVGFANGC
                     FDLIHPGHVRLLSEARGACDRLIVALNTDASVKRLKGPSRPLQNEMARATVMASMAPV
                     DLVTLFDEDTPLEMIRALRPDILVKGSDYTVDQVVGADLVKSWGGKIVLVTLRDGHST
                     TGTINRMTAVA"
BASE COUNT         1322 a         2274 c         2185 g         1177 t
ORIGIN      
        1 gttcgtgccg atcggcctgg tcgccgccgg tgcttcggat ccgaccaatc cttgtcccac
       61 caccggttgc atcgcggtct tcttgccttc gtcgatcgac gctgacgacg gcaagcggca
      121 tgaagaccgg cgttcggacg acgatgacaa agacagatag ccgccctggc ctgaagtaat
      181 catccggcgc ccgcggtagt ccccgcgggc gcttttcgtt gcggccgagc agcagaaatc
      241 aacaaatgtg gacccgcaca aaagcgtgag tcttcgttct gtaagacagg tgtcagcgga
      301 acgacttaca acccaaccat aacggcactg gccgacccta atgcccacag gggaacaacc
      361 aatgcacctt cgaacattca gagcaaggct cttgctatgc tcatcgctgt tcgcgatgac
      421 cagtcaggtc gtcgcacctt cggtcctggc ggcgacaccg cccgggatgc cgcccgggac
      481 gccgctggga acgaacgacc tgaacaccaa gacccagatc aagcatgtca ttatcatcta
      541 cggcgagaac cgaagcttcg atcacgtttt tggcacgtat cgttcgccgt cgggcgacac
      601 cgtgtccaat atcctctcga aagggatcgt caacgaagac ggcacgcccg ggccgaactt
      661 cagcaaggcg gctcagttcc aggccaaaac caccgacacc tacagcgtaa gcccaggcaa
      721 gacgtcggtt taccagaccc tgccgccgcc gttcgccacg atccaggcgg cggagaacgc
      781 ggactacggc ttgctgcccc gcgatctgcg gctgctgacg acgggcgcca ccggcttgaa
      841 gccgggatcg gtcgataccc ggatcgcgaa cgcgaatgcg ctgccaagcg ggccgtatca
      901 gttgacgccg ggtcagccct acgacgccta cgccgccagc cccgttcacc gctactacca
      961 ggctcgccag caggccgact gcgacgcatc gaaggccagc gaagccgatc ccagcggttg
     1021 cgcgaacgac ctgtatccgt gggtcgaggt gacgatcggc gccggcagca atggcaagcc
     1081 acagcccagc ggcttcaatg acgccacaac gggtgagggc tccaccgcga tggggttcta
     1141 caacgtccag caaggcgaca tggcgtactt caagaagctt gccgacgaat acaccatcag
     1201 cgacaactac catcagcccg gcatgggcgg caccgggttg aacagcatcc ccccggtttc
     1261 gccaacggca tctggtactc cgacggaaag ggcagcgccg ccacaccgcc agccgaccag
     1321 atcgagaacc caaatcccca gcccggcacg aacaactggt acacccagga tggctactcg
     1381 ggcgggtcgt acagtgcctg cgccgatgcc agccagcccg gcgtcggttc ggtcgtcggc
     1441 tacctgaaag ccctgccgaa gcctgtcgcc gccaattgcg aggacgggca ttactatctg
     1501 ctgaacaact acaatcccgg ctacttcggc aacggaacgg tggacaccac cgataccttc
     1561 accattccgc cggtgccgac gcccagcatc ggcaacgtgc tgctggcggc cggcgtgtcg
     1621 ttccgctggt atggcgaagg gtttacccag tacgccgccg acccggcgga cccaaccaac
     1681 gtgtactgca acatctgcaa tccgttccag tatcagacct cgatcatggc gaacgagacg
     1741 gtacgggacc aggtgctcaa ggacaccacg gagttctaca ccgaccttca ggccggcgtc
     1801 ctgccggcgg tatcgttcgt gaagccgggg gcgctgaacg acggccatcc ggcatcctcg
     1861 aagttcagca tctatgaggc gttcgtccgc aagatcatca ccgaactgcg caagcagccc
     1921 aagctttggc aatccaccgc ggttttcatc acggtggatg aggctggcgg atactacgac
     1981 tcgggttaca tccagccggt ggacttcttc ggcgacggac cgcggatccc gctgatcgtc
     2041 gtgtcgccat tcacccgcgg cggccgcgtt tcgcacgagt atggcgacca cgtttcgatc
     2101 ctgaagttca tcgaggcgaa ctggggtctg ccgacgatct ccggccgaag ccgcgacaac
     2161 ctgcccaacc cggtgcagtc gggggccaat ccgtatgtcc cgaccaacgg accgtcgctt
     2221 ggggacctta tgtcgatgtt ccagcgatcc aacgacgaca ggcacgacga cgcgaacgac
     2281 gactgacgtc ggcccgaaga cggttcggtg ccgaacagcc aaaggcggcg ataagcgttg
     2341 aggcggcgat tgcacggggc cgtctggctt cgtgcaatcg atctcgtgcc cagtgccctg
     2401 agcgcgaagc ctccatcccg aagcggcctg cccgtagcct cttggcaccg ccgggaagca
     2461 cacgcgcatg gcacggcgga acatccgcta cggcggcgtc cattcttgtt ggccgacgac
     2521 gattatcgcg cgaatgcgcc cctggtatca cgcgaatgcg ccctggatca caagggattg
     2581 cgtcgctccg gctgccggtc cggtccatca gtgctttggg cagggcgacg gatggaacga
     2641 aactaatgcg aggcaacatc gttcgccggc agcggtcgct agctcttgtt ctgggcctgg
     2701 ttcaacttgc gggctgcaat cacccatccc ccccggcgat cccagcattg tccccgttgc
     2761 cgaacggcgt caccgccgcc ccggcgcggg ttgacggcgg cgtaggcgcc cctgacgcac
     2821 tgccgccggc gcaggtcgca tatggaacaa atgccgcgat cccgccagcg ggttcgccgg
     2881 ccaacgccga accgggggac gtcagcctgg atttcgccga taccgacatc cgggaggtcg
     2941 cggcgcagat cctgggaacg atcctgaagg tcaactacac catcgacccg tcaatccatg
     3001 gcaccgccac cttgcatacg gtccggccat tgaaccggtc ggaactggtc ccgacgctgg
     3061 aatcgctgct ggcgcagaat ggcgcggcgg tggtgacgaa tggcagcctt tatcgagtcg
     3121 tgcccatcgg ccaggcgatc gtcaccgcgg cgagcggggc cggaaccgcc ggtgccgtcg
     3181 tcgtgccgct gcgctacgcg acgtccgagg atttggccaa gatcctgcaa ccctatgtcg
     3241 gcgaaggtgg caagatcgct gccgacccgg gacggaacgc gctgttgatc tcgggcgaac
     3301 ccgccgcccg agaagggttg atcggcctgg tgcgcgcgtt cgacgtggat gtgctggccc
     3361 ggcaatccta cgcgttgctg ccggtgaaca acggcgacgt gaaggatttc acctcggcgt
     3421 tgcaggacgc gttcaggggg caagccggcg gcgcattggc ggggcttgtg cgcgttgtcc
     3481 cgctggcgcg aatcagcgcc gtgctggtcg tatcttcaca gcaacggtat atcgaagagg
     3541 ttcgccgggt ctatacgctg atcgagcggg cgcgcaggca gaccatccga agctggcatg
     3601 tgtactatct gcagaacagc cactcccagg acgtcgccta tgtgctgcag caagcgttca
     3661 cgcccggcaa cgtcacggcg cagccctcca gcaacacgcg ttcccagacc ggacaatcag
     3721 gaaccggaca atcgggatta ggaggatctg gcggagccat cggcggcggc ggtggcggcg
     3781 gtggcctggg aagcgggtcg gccggctcgc tggggcggtc cgggctgtcc ggcggcatca
     3841 gcggtggggg cggcggtttc ggcggcggcg gtggcggttt cggcggtggt ttggggcaag
     3901 ggggcgggca ggggggcgga cagcaggcga tcggcggccc gccgcagcag gcccagcaat
     3961 cggtagcggg cggtgccaac ccattgctcg gcggcttgga accaggaggc tccgatcagg
     4021 gaaccgacac gttgcgggtg atacccaacg atcagaacaa tgccatcctg gtctttggca
     4081 ccgagcgcga acagaacacc atggaagcga tgttgcggaa gatagatatc cttccgttgc
     4141 aagttcgtat cgatgccgtc atagccgagg tgacgctgaa cgataccctg gaatacggca
     4201 cacagttctt cttcaagtcg ggcggcatca acggcatcct gaacaccgcc gcgcagacgc
     4261 cggtcaacgc agccgggacg gcgctgaacc tgaacttccc gggtttcttc ctgggtggca
     4321 gcagcagggg cggggcgcca ttcgcgatcc aggcgctgca acaggtgacc acggttcacg
     4381 tcctatcgtc gcccgaactc ctggttctgg acaaccagcc ggctcggctt caggtcggca
     4441 gtgtggttcc ctatctcagc cagtcctcgc aaagtaccat ctcgccgaat gccccggtgg
     4501 tcagctcgat caactatcag cagaccgggg tgatcatgga ggtcacaccg cgggtaaata
     4561 gtggtggcct ggtgacgctg gatgtgatgc aggatgtcag cagcgtcgcg accgggatca
     4621 ccacgccggg catcaattcg ccgacgttca acgagcgcaa cgtgacctcg cgcgtggtgg
     4681 tgcaggatgg ccagacgatc ggcctcgcgg gactgatcac cgatagcgct tcgctgggca
     4741 accaggggat tccgtggctg aaagacgtgc cgatcctggg gctgctcgcg ggcacgcaga
     4801 acaacacccg gcagcggacg gaattgctga tcctgattac cccgcatgtg atccacgacc
     4861 aacgcgacgc gcgggcactg accgatgatt tgcgcgatca gttgatcaac gcggccgctg
     4921 tgccgggcat gttgaacggc ttgcgtccct cgggtcagtc agacccctcg gctcgccttc
     4981 ggaaacaact ggacctgcaa caatagtttt ctgacgacat gcgcgcgaac gctgtcggct
     5041 tactggttgc agatttcctg atcgccgccg gtgccaagcg gcccttggga gcacaggtcg
     5101 aagtcgtgac ccgcgcgggc tgatggcccc cggtacacgt atggatggtt ccacggatcg
     5161 accggcatgc cgcccttcac atacggaccg ttccagttgg aaatccccga gggaggctcg
     5221 atcaacgccc gcaggccctg gtcgctggag gggtagctgc caatatccag tttgtacaga
     5281 tccagcaccg aactgatgcg ctcgatcgac tgcttggcga ccgacgttct ggcgctgccg
     5341 agctggcgca gggccgcggg ggccacgaga ccgatcagca ggccgaggat ggcgatcacc
     5401 accaacagct cgagcaaggt gaagcctcgg tcgtcacggc ggggacggtt gctcatcgat
     5461 tgaccaatct gttgttgtcc tgtcgcttcc cgttcggctg ccggctgaac aacgcgccgg
     5521 ccgaggacag caccttcaac tgataagcgg agccgagcgc gggtgtgtgg gaatggtaaa
     5581 agccgatggc ggtcatcagg tcgccccgtg cctcggtcag ataaatccgc atgatcgcgg
     5641 ctgaagctgc gatgttgaaa catgggtcgt cgatcaggcg atcgatcgtg gcctgctgcg
     5701 tcatatgagc ataagcggcc agcggttcga tccaaatggt gttgatctgc atcagtccca
     5761 gatcggcgga gccattggcg ttgggctgga ccagtcccgg ccggccgccc tcgaccgcct
     5821 ggatcgaggg aagcacgcgc ggcggcaggt gatagaacgc cgcggccgcg gccatgcacg
     5881 ccaggaatgg gatgctcatg gaagccccga attccgcact aagccaccgc cgtcatgcgg
     5941 ttgatcgtac ccgtggtgct gtggccgtcg cgcagcgtga ccaggacgat ctttccaccc
     6001 cagctcttga ccagatcggc gccgaccacc tgatcgacgg tataatccga tcctttcacc
     6061 agaatatccg ggcgcagcgc acggatcatt tccagtggtg tgtcctcgtc gaacagcgtg
     6121 accagatcga ccggcgccat cgaagccatg accgtggcgc gagccatctc gttctgcaac
     6181 ggccggctgg gccctttcaa ccgcttgacc gatgcatcgg tgttcagcgc gacgatcagg
     6241 cggtcgcacg ccccacgcgc ttcgctgagc agccggacat gaccaggatg gatcaggtcg
     6301 aagcagccat tggcgaagcc gactttcagg ccccggcggc gccactccgc gaccctttcg
     6361 acggcgtcct cgagattggc gaccttgcga tccgtctcga tcagctcatg cagatgcagt
     6421 acatccagca gttcctgacg cgacaccgtg gcggttccct gcttgcccac cgagatgccg
     6481 gcggtggcgt tcgccagcat cgccgcatcc ggcaacgacg cgccggcgcc caaagccacg
     6541 gccagagccg ccaccagcgt gtcaccggcc cccgaaacat cggcaacctc ccgcgctcgg
     6601 gtggcgagat gaagtgcgtc ggcatcgcgg cataccagcg tcaggccctt ggccgatcgc
     6661 gtgaccacta ccgcccgccc gccggttgcc tccagcgcca tgcgtccggc gcgatccgcc
     6721 tcggcatcgt gttcggaatc gatccgcgtc gccacgcgga cttcgtgttc gtttggggtc
     6781 agcaccgtgg caccgcgata cgcggcgaaa tccgcccgct tggggtcggc gatgatcagc
     6841 cggcccttct cgttgccgcg cgccaggatc gcatccagca ccccgtcgca cagcacgccc
     6901 ttggcataat ccgacagcac aatcacatcc gccgcgtcga gctgctgttc aaccgcct
//
LOCUS       DASWQN010000623         4503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1510710, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000623 DASWQN010000000
VERSION     DASWQN010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510710"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..772)
                     /locus_tag="VGC82_10595"
     CDS             complement(257..772)
                     /locus_tag="VGC82_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423760.1"
                     /translation="MPDSVADIAQRHGFSPDAARAVAEALRHGGGRMAQFNHPELGGM
                     GQWVAGGMLMIGDMFNNDLKARVDALCRDLAAAPGPVPATTEPQPGQTSHWWPDGLGS
                     ASATGAQNDMRYACFPDQRRLAVMRDGRVRVYDTGDHRISGFSQQQSSSQSLSFTSQT
                     GIVRLEDLHEV"
     gene            1024..1479
                     /locus_tag="VGC82_10600"
     CDS             1024..1479
                     /locus_tag="VGC82_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0423761.1"
                     /translation="MIALNINGQVHQLEVDPETPLLWVVREWLGMTGTKFGCGIAQCG
                     ACSVHIDGAVTRSCSVPVGSVGTAKVVTIEGLSPDGISHPVQRAWLAHEVPQCGYCQS
                     GQIMAAAALLAQNPKPSDADINDAMSNICRCGTYNRIRAAIHTVAAGAA"
     gene            1490..3661
                     /locus_tag="VGC82_10605"
     CDS             1490..3661
                     /locus_tag="VGC82_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0423762.1"
                     /translation="MELNRRLFVATSVAGAFSLGWKMPASAQAVALPSAQGAAPNGAG
                     EVGIWAVVHPDDTVVVRIARSEMGQGTFTGLAQLVADEMDADWPKVRAEYITPEANLA
                     SKRAWGDMSTGGSRGIRSSVDYVRKGGAAARAMLIRAAAEQWGVPIDQCTASSSVVTH
                     TPSGKTVRYGEVAAAAAKLSVPTEVTLKKPAEWKIIGKGVKRLDTVEKLSGKQIFAID
                     VQLPDMLNATIAQCPVFGGKLVSFDAKAIQGMAGVRQVVAVGDNAVAVVADKFYQAKT
                     ALAKLPIVWDEMGNGQVSSATIAATLREGLEAKQAGEGLRQGDVDAAFAGAAHKLEAV
                     YGTPFLNHATLEPMNCTAKVVGETVEIWVPSQNGEASMAAAAEAGGVKLADVKVHKLH
                     LGGGFGRRGQQDYVKQAVMIAKQVGGRPVKLIWTREEDMQHGFYRPVGLAKMSGAMDD
                     AGRVVGLRMRVAAPSIVANLIPSALVNGVDKVAFQGMNPDEFGYMAVPNILIDHAIRT
                     TSVPVGFWRGVNVNQNTICMECFVDEMAYAAKQDPLEFRRAMMGKNPKHLAILNAVAD
                     KAGWGTKPPNGVYRGIAQNCGFGSYVAAVAEVSVSDKGELKILRIVAATDPGYAVNPD
                     QIDAQVDGSFAYGLSAMLYSENTIKNGRVVEGNFDTYQVARMSDMPKVETVLAPSGGF
                     WGGVGEPTIAVAAPAVLNAIFAATGKRIRQLPLKDQDLRRT"
     gene            3987..4142
                     /locus_tag="VGC82_10610"
     CDS             3987..4142
                     /locus_tag="VGC82_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423763.1"
                     /translation="MPINGRDAGDLSASMEAFRSGERPSTLMGRLMKGFSRDEIQAIA
                     AWTAAQK"
     gene            4152..>4503
                     /locus_tag="VGC82_10615"
     CDS             4152..>4503
                     /locus_tag="VGC82_10615"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0423764.1"
                     /translation="MLERRSMLTATAAALAMPFVPRPGVAQAAARVVVIGGGFGGASV
                     ARALRKTDPRIDVTLIEPNPVFTACPFSNEVIAGMRDIAKQRFSYQGIAASGVHVVQA
                     AAIGVDPPSRRVTVS"
BASE COUNT          825 a         1406 c         1480 g          792 t
ORIGIN      
        1 acgcctgttg caacggcacg atcatcacgc ccggcgcggc ccgcacgcca accccggcca
       61 gcatcgaaaa gaacaccaca accaaggtaa tccaggcata gtcaacccgg ccggagcagg
      121 cttttgcgag gcgtgcgtgg atcatgcgaa ggatcttctc tcaccagtcg aaatctgtag
      181 aacggtgtca ccagtcaagg tagcgtcacg tcttgcctac caagcagtat ttattgcgcg
      241 ctggttcgcg ttgatgtcaa acctcgtgca ggtcctccag ccgaacgatt ccggtctggc
      301 tggtaaagct caggctctgg ctactgcttt gctgctggga aaacccactg atccggtgat
      361 ctccggtgtc gtaaacccgg acgcggccgt cgcgcatcac cgcgaggcgg cgttggtcgg
      421 ggaagcacgc atagcgcatg tcgttttgcg cgccggtggc ggaggccgat cccagaccat
      481 ccggccacca atggctggtt tggcccggct gtggttccgt ggtggccggc accggaccag
      541 gcgcggccgc gaggtccctg cataaagcgt ccactcgcgc cttcaggtcg ttgttgaaca
      601 tgtcgccgat cataagcatg ccgcccgcga cccattggcc cataccgcca agttccgggt
      661 gattgaactg cgccatgcgg ccaccgccgt gacgcagagc ctccgccacc gcgcgagcgg
      721 catccggact gaatccgtgg cgctgggcga tgtcggcaac gctgtctggc atgatgcggt
      781 ctcctgtgat gtcctgaatg tggaaggtcg tggtccgcgg cggtttatgc gccggccaac
      841 cggttccacg ccccaacatc cacatggttc cggcgctggc cgcttgcacc ggttaacttt
      901 gacgatcagg ataccgtctg atcacatggc cggccagctt ccggtcattc caactgcgtg
      961 acgccagtcg acctacgcca gtcgactcac accagtcgcc atacgccaac cagggggttc
     1021 aggatgatcg ccttaaacat caatgggcag gtgcatcagc tggaggtcga tccggaaacg
     1081 ccattgctct gggtggtccg cgaatggctt ggcatgactg gcacgaagtt cggctgtggc
     1141 atcgcacaat gcggcgcgtg ctcggtccac atcgatggcg cggtgacgcg ctcgtgctcg
     1201 gtgccggtcg gatcggttgg caccgccaag gtggttacga ttgaaggact gtcacccgac
     1261 ggcatcagcc atcccgtaca gcgggcctgg ctggcacacg aggtgccgca atgcggctac
     1321 tgccagtccg gtcagatcat ggcggcagcc gcgctgctgg cacaaaaccc gaagcccagc
     1381 gacgccgaca tcaacgacgc gatgagcaat atctgccgct gcggcaccta taaccgcatc
     1441 cgcgcggcga tccacaccgt cgccgccggc gcggcatagg agcggagcca tggaactcaa
     1501 ccgtcgcctg ttcgtcgcaa cctcggttgc cggcgccttc tcgctcggct ggaaaatgcc
     1561 ggcgtccgcc caagccgtgg cactcccgtc cgcgcaaggc gcggcgccaa acggtgccgg
     1621 ggaagtcggc atctgggccg tcgtccatcc cgacgacacc gtcgtcgttc gcatcgcacg
     1681 ctccgagatg ggtcagggaa cctttaccgg actcgcccag cttgtcgcag acgagatgga
     1741 tgccgattgg ccgaaagtcc gggcggagta catcacccca gaggccaacc tcgcgagcaa
     1801 gcgcgcctgg ggcgacatgt ccaccggcgg cagccgcggg attcgatcgt cggttgacta
     1861 tgtccgcaaa ggcggtgccg cggcacgggc catgctgatc cgcgccgccg ccgaacaatg
     1921 gggcgttcca atcgatcaat gtaccgcgtc gtccagcgtc gtcactcata cgcccagcgg
     1981 caagacggtc cgctatggcg aggtggcggc ggcggcggcg aagctttcgg tgccaaccga
     2041 ggttacgctg aagaaaccgg ccgagtggaa gatcatcggc aagggcgtga agcggctcga
     2101 tacggtcgag aaactgtcag gcaagcagat cttcgccatc gatgtgcaat tgcccgacat
     2161 gctgaatgcg accatcgcgc aatgcccggt gttcggcggc aagctggtca gcttcgacgc
     2221 gaaagccatc caggggatgg caggcgtccg ccaggtggtg gcggtcggcg acaacgccgt
     2281 ggctgtcgtc gccgacaagt tctatcaggc caagaccgcg ctggcgaagc tgccgatcgt
     2341 gtgggacgag atgggtaacg gccaggtcag cagcgcgacg atcgcagcca cgcttcgcga
     2401 agggctggag gcaaagcagg ctggcgaagg cctcaggcag ggcgatgtcg atgcggcatt
     2461 cgccggagcc gcgcacaagc tggaggcggt gtacggcacg ccgttcctca atcacgcgac
     2521 ccttgagccg atgaactgca cggcgaaagt cgttggcgag acggtcgaga tctgggtccc
     2581 gtcgcagaat ggcgaagcat cgatggccgc cgctgctgaa gctggcggcg tgaaactggc
     2641 tgacgtgaag gtccacaagc tgcatttggg cggcgggttc ggccggcgtg ggcagcagga
     2701 ttacgtcaag caggccgtca tgatcgccaa gcaggtcggc ggacgcccgg tgaagctgat
     2761 ctggacgcgc gaggaggaca tgcagcacgg cttctatcgc cccgtcggcc tggcgaagat
     2821 gtcaggcgcg atggacgatg ccggtcgtgt tgtcgggttg cggatgcggg tcgccgcacc
     2881 ctccatcgtc gccaacctga ttccgtcggc gctggtgaac ggggtggaca aggtcgcctt
     2941 ccagggcatg aatcccgatg agttcggcta catggcggtg ccgaacatcc tgatcgacca
     3001 tgcgatccgg accacctcgg tgccggtggg tttctggcgt ggcgtcaacg tcaaccagaa
     3061 caccatctgc atggaatgct ttgtcgatga aatggcctac gcggcgaagc aggatccgct
     3121 ggaatttcgg cgcgcgatga tgggcaagaa tcccaagcac ctggcgatcc tgaatgccgt
     3181 tgcggacaag gccggctggg gcaccaagcc gccaaacggc gtgtatcgcg ggattgccca
     3241 gaattgcggg tttggcagct acgtcgcagc cgtggcggag gtttcggtgt ccgacaaagg
     3301 cgagttgaag atcctgcgta tcgtcgcggc caccgatcca ggttacgcgg tgaaccccga
     3361 ccagatcgat gcacaggtgg atggttcctt cgcttatggg ctgagtgcga tgctctacag
     3421 cgaaaacact atcaagaacg gccgggtggt cgagggcaac ttcgacactt accaggtggc
     3481 gcggatgtcc gacatgccga aggtcgagac tgtcctggca ccgagcggcg gtttttgggg
     3541 cggggttggg gagccgacga tcgcggtcgc ggcacctgcc gtgctcaatg cgatcttcgc
     3601 tgccaccggc aagcggattc gccagttgcc gctgaaggac caggatctgc gccgaaccta
     3661 gcgtccgcca atgttccgcg tcggttgcgg tgcggcttgt tcgcgcagcc gccaggatca
     3721 ttaaaccaga cctgttgtcg ctgattccaa tgtgtcacga ccggccttgt gccggtcact
     3781 cgcgacttcg cctagttcgg taccgcaggt cgtgcatgcc cgccaagcac ggccatgaca
     3841 ggaaacgact gaccgccatg ttaagaaccg ccgctttgct gctgttggtg ccttgtctcg
     3901 cggtggcggc tgatctgccg gccggaccac ctcagggagc cacgagctgt tcgggctgtc
     3961 atgcgccagc cggcagcggc ggtttcatgc cgatcaacgg gcgcgacgca ggcgacctgt
     4021 ctgccagcat ggaagcgttt cgcagcggcg agcgcccatc cacgctgatg ggccgcttga
     4081 tgaaagggtt ttcgcgcgac gaaatccagg ccatcgcggc ctggaccgcc gcacaaaaat
     4141 aggagcggcc aatgctcgaa cgccgatcga tgctgacggc aaccgccgcg gcgctggcca
     4201 tgcccttcgt tccgcgccct ggtgtggcgc aggcggcggc gcgggtcgtc gtcatcggtg
     4261 gcgggttcgg tggcgcaagt gtcgctcggg cgctgcggaa gaccgatcca cgcatcgacg
     4321 tgacgttgat cgagcccaac ccggtcttca ccgcctgccc cttcagcaac gaagtgatcg
     4381 cgggcatgcg cgacatcgcc aagcagcgct tttcctacca gggcatcgcc gcatccggcg
     4441 tacatgtggt gcaggcggcg gcaattggcg tcgatccgcc gtcccgccgc gtcacggtca
     4501 gca
//
LOCUS       DASWQN010000624         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_455681, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000624 DASWQN010000000
VERSION     DASWQN010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455681"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..1129
                     /gene="xth"
                     /locus_tag="VGC82_10620"
     CDS             326..1129
                     /gene="xth"
                     /locus_tag="VGC82_10620"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0423765.1"
                     /translation="MRIATYNVNGVNGRLPVLLRWLAWAQPDVVCLQELKAPQEKFPE
                     AAIRGAGYGAIWHGQKSWNGVAILARGAEPQETWRGLPGDTDDSQSRYLEANVAGILV
                     GCLYLPNGNPAPGPKFDYKLRWFERLREHAAGLLAQNAPVVLAGDYNIMPTNLDVYKP
                     ERWVHDALFRPEVRTAFHELVAQGWTDALRQLYPDKRIYTFWDYFRNAYGRDAGLRID
                     HLLLSPKIAPRLRAGGVDREVRGWEKTSDHAPAWIELADVPAPRQRRLG"
     gene            complement(1172..2767)
                     /locus_tag="VGC82_10625"
     CDS             complement(1172..2767)
                     /locus_tag="VGC82_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0423766.1"
                     /translation="MRRRTLLTSGSVAAAAGALPSRFAIGQTLAGTNKLLRYIPTAVL
                     SVLDPSWTTTQVSITHGYYVFDTLFGLDSSQTPRPQMAERADVSDDGRTWTIRLREGL
                     RFHDNEPVLARDAAASLRRWAARDVYGQTVGAVVEEWGAADDRTIRIKLTVPFPLMLD
                     ALAKPNGMLPVVMPERLANTDLAKPVTEMVGSGPYRFLKNEFVPGSSGAYEKFAGYVP
                     RTEKPDWASGGKVAHIERIEWRIINDPSTASAALQKGEVDWWEQVQPDLLPLLRKNAD
                     LTVANFNPVGFFGCMRFNHLHPPFNNAAVRRAVRLGMNQEDYMSAVTGGDAAIYRSCK
                     ALFPCGTPYGEEIGAEDMTGDVAAARAALKQAGYAGEKVVILNPADVPTIAPFGQVTF
                     DYLKQLGMNVEIQEMDWNTLAQRRTKADPVDKGGWSIFHNWWLGTSIANPAISTVVRG
                     LGAKGWAGWHSNEKIEALTADWTHASTDEARHSLARSIHQEAMNSVPTVVLGRFFILT
                     AHRKGLRGLLQGTSPYPWNLSWT"
BASE COUNT          500 a          910 c          855 g          529 t
ORIGIN      
        1 ccctccccca ccaagcggac tggacaggtg tgccgcatcc cccaatagga aacaccgtcc
       61 atcggtcaga cgttcgacca cacgcttgtg catgttgaac ggggaaacca acccaaattg
      121 ctcaacccta cgtcgacgcc gatccgagca tcgagcaaag cgcccaactc aactgctgtc
      181 ggaagctcgc ttcgcgtatc cgtctcgtcg cgattgacaa aagtgctatt cccaaaaggc
      241 agccttcttc gcttgccggc cgtttcgggc catagcacca tctgataccc tgaggacgcc
      301 ccagcatgat ccgcggtttc gcggcgtgag aatcgccacc tacaacgtca acggcgtgaa
      361 cgggcgcttg cccgtgctgc tgcgctggct cgcgtgggct caaccggacg tcgtttgcct
      421 gcaagagctg aaggcgccgc aggagaagtt tccggaggcg gcaatccgtg gggccggcta
      481 cggtgcgatc tggcacggcc agaagagctg gaacggtgtc gccatcctgg cgcgcggtgc
      541 tgagcctcag gagacctggc gcgggttgcc gggcgatacg gatgacagcc aaagtcgcta
      601 tctcgaggcc aatgtcgccg gaatcctggt cggctgcctc tatctgccga acggcaatcc
      661 cgcccctggg ccgaagttcg actataagct gcgctggttc gagcgtctca gggaacacgc
      721 cgctggcctt ctcgcgcaaa acgccccggt cgtgctggcc ggcgattaca atatcatgcc
      781 gaccaacctc gacgtctaca agcccgagcg ctgggtgcac gacgcgctgt tccgccccga
      841 ggttcgcacc gcctttcacg agctggtggc gcaagggtgg acggatgcgc tccgacagtt
      901 atacccagac aaacgtattt acacgttctg ggattatttc cggaatgcct atggccgcga
      961 cgcggggttg cggatcgacc atctgctgct ttccccaaag attgcgcctc gcctgagggc
     1021 cgggggcgtt gatcgcgagg tgcgcgggtg ggagaagaca agcgatcatg cgccggcctg
     1081 gatcgagttg gccgatgttc ctgcgccgcg ccagcgccgt ttgggctgac cagctggctg
     1141 gcgcgatcca ccgcgtgcga ccaattcggc atcatgtcca ggataaattc cacgggtagg
     1201 gggaagttcc ttgtagcagt ccacgcaatc ccttccggtg tgcggtcagg ataaagaacc
     1261 gtcccaacac caccgtggga accgaattca tcgcttcctg atggatcgac cgcgccaggc
     1321 tgtgacgagc ctcatccgtc gatgcgtggg tccagtcggc tgtcagcgcc tcgatcttct
     1381 cgttgctgtg ccacccagcc cagcccttgg cgcccaaccc gcgcaccacc gtactgatgg
     1441 ccggattggc gatcgaggtg cccagccacc agttgtggaa gatcgaccac ccgcctttgt
     1501 caaccggatc ggccttcgtg cgccgttgcg ccagggtgtt ccagtccatt tcctgaattt
     1561 cgacgttcat gccgagctgc ttgaggtagt caaaggtcac ctggccgaac ggcgcgatgg
     1621 tgggaacgtc ggccggattc aggatcacca ccttctcccc cgcatagccg gcctgcttca
     1681 gcgcggcacg agccgccgcc acgtctcccg tcatgtcttc cgcgccgatt tcctccccat
     1741 acggcgtgcc gcaggggaat agcgccttgc aggaccggta gatcgcggca tcgccgccgg
     1801 tcacggcgct catgtagtct tcctggttca tgcccaggcg caccgcccgg cgaacggcgg
     1861 cgttgttgaa gggcggatgc aggtggttga accgcatgca gccgaagaat cccacaggat
     1921 tgaagtttgc caccgtcagg tcggcattct tgcgcagaag cggcagcaga tcaggctgca
     1981 cctgctccca ccagtccact tctcccttct gcagcgcggc cgaggcggtc gaagggtcgt
     2041 tgatgatccg ccactcaatc cgctcgatat gcgcgacctt gccgcccgaa gcccagtctg
     2101 gtttttcggt ccgcggcaca tagccggcga atttctcgta agcaccgctg ctgcccggga
     2161 cgaactcgtt tttcaggaaa cggtatggcc cggagcccac catctccgtg actggtttgg
     2221 ccagatcggt gttggccagt cgctccggca tcaccaccgg cagcatcccg ttcggcttgg
     2281 ccagtgcatc cagcatgagc gggaagggga cggtcagctt gatgcggatg gtgcgatcgt
     2341 ccgccgcacc ccattcttcc acaacggcgc caaccgtctg gccataaacg tcgcgcgccg
     2401 cccagcgccg caggctggcg gctgcgtcgc gggccagcac cggctcattg tcgtggaagc
     2461 gcaatccctc gcgcagcctg atggtccagg tgcggccgtc atcggagaca tcggcgcgtt
     2521 cggccatctg cgggcgcggt gtttggctgc tatcgaggcc aaacagcgtg tcgaacacgt
     2581 aatacccatg ggtgatggaa acctgcgtcg tggtccagga tggatccagc accgacagaa
     2641 cggccgtcgg gatatagcgt agcagcttgt tggttcccgc cagggtctgg ccgatggcga
     2701 atcgtgacgg caacgccccg gccgcggcgg caacggagcc cgatgtcagc agagtgcgtc
     2761 gccgcatgtc ttgaatcctc attttcccaa ggca
//
LOCUS       DASWQN010000625         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2567433, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000625 DASWQN010000000
VERSION     DASWQN010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2567433"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1610
                     /locus_tag="VGC82_10630"
     CDS             <1..1610
                     /locus_tag="VGC82_10630"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0423767.1"
                     /translation="YSAPPVEREPLADACEVLVIGAGFAALLLWHKLRDAGVLDVRFC
                     EKGGDVGGTWYWNRYPGIACDVESYSYLPLLEEMGYIPTMKFASGFEILEYCQSMAEK
                     SGFYDHCLFHTTVQQTVWDEAAGCWTVLTDRGDKMRARYVVLANGILTTPKLARIEGM
                     ETFQGQAFHTSRWDYTIDLEGKRIGIIGTGATAVQVIPEIAKVAKKLYVFQRTPSTID
                     VRDQRATTQEEIETWSKDPGWAKARRERLARISSGRTALKGNDDYLSGKVDTFKERRQ
                     YERELSQEELIHKQLNTNFRIMEQIRARIDAIVADPKTAAALKPYYPYGCKRPTFHDE
                     FLPTFNLPHVTLVDTAPLGVSRINTRGVVHEGTEYPLDVLIYATGFQWMATSTFNMIT
                     GRFGQTLRQKWESDGVRTFLGLHSHGFPNLFIMSGPQGGGGQFNFTRGIEAHTDYVVW
                     MLKTLRERGANIVDIKKQPENAYAEHCREADISTRPLRDCLSYYNGDGAAEPGSLAYY
                     GGPQKWHQLRSAAQESLEPYVFEVAPA"
     gene            complement(1712..>2317)
                     /locus_tag="VGC82_10635"
     CDS             complement(1712..>2317)
                     /locus_tag="VGC82_10635"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423768.1"
                     /translation="GQSWDIITDLAIKLVPGGHPYHAFAEAAANAARRGKMAAPEVES
                     ITVSRPGLTVLGGPRHPTDLIEMAHSLAYFAAAGAADHEFTWKHASAAKIGDPAIHML
                     IDKVQVGSPPIENTVRYRQGATVAIRTYDGRISTDTVYLPKGAGALGIAWSDIDGKFR
                     TLLPEAGMHDDQIEATLALIHDFGNLANVSVLTGSLRRRDV"
BASE COUNT          489 a          700 c          695 g          433 t
ORIGIN      
        1 tctactcggc gccccccgtc gaacgcgagc ccctcgccga cgcgtgcgag gtcctggtga
       61 tcggggccgg cttcgccgct ctgctgctct ggcacaagct tcgcgacgcc ggagttctcg
      121 atgtccgctt ttgcgagaag ggcggcgacg tcggcggaac ctggtactgg aatcgctatc
      181 ccggaattgc ctgcgacgtg gaatcgtaca gctatctgcc tctgctggag gagatggggt
      241 acatccccac tatgaagttc gcctcgggct tcgagattct cgaatattgc cagagcatgg
      301 cggagaaatc cggcttctat gaccactgcc tgtttcacac gaccgtccaa cagacggtgt
      361 gggacgaggc tgccggctgt tggacagtcc tcacggaccg cggcgacaag atgcgcgccc
      421 gctacgtcgt gctggccaat ggcatcctga cgacaccaaa gctggctcgg atcgaaggca
      481 tggaaacctt ccaaggccag gcgttccata cctcccggtg ggactacacc attgacctgg
      541 agggcaagcg catcggcatt atcgggacag gtgcgaccgc cgtgcaggtc atcccggaaa
      601 tcgccaaggt cgcgaagaaa ttgtacgtct ttcagcggac gccctccacc atcgacgtgc
      661 gcgaccagcg cgcgacgaca caggaggaaa tcgaaacgtg gtcgaaagat cccggttggg
      721 cgaaggccag acgtgaaaga ctcgccagga tatcatccgg gcgaaccgct ctgaagggca
      781 atgacgacta tctctcgggt aaagtggaca ccttcaagga acgcaggcag tacgaacggg
      841 aactgtccca ggaagaactg atccacaagc agctcaatac caacttccgg atcatggaac
      901 agatcagagc acgaatcgac gcgatcgtgg cagatccgaa aaccgcggcg gcgctcaagc
      961 cctactatcc gtatggctgc aaacggccga cgttccacga cgaattcctg ccgacgttca
     1021 acctgcctca tgtgacgctt gtcgataccg ctccgctggg tgtcagcaga atcaacacca
     1081 gaggcgtggt tcatgagggc accgagtacc cgcttgacgt tctgatctac gcgaccggct
     1141 tccaatggat ggcaacgtcc acgttcaaca tgatcaccgg ccgcttcggg caaaccctgc
     1201 gacagaaatg ggagtcggac ggggttagga ccttcctcgg tctgcacagt cacggattcc
     1261 caaacctgtt catcatgtcg ggaccgcaag gcggcggcgg acagttcaat ttcacccgcg
     1321 gaatcgaagc tcatacggat tatgtcgtct ggatgctgaa aacattgcgc gagcgcggtg
     1381 ccaacatcgt tgatatcaag aaacaaccag agaacgctta tgcggagcac tgccgtgaag
     1441 cggacatcag cacccgtccg ctgcgcgatt gcttatccta ctacaatggc gacggcgccg
     1501 ccgaacctgg cagcctggct tactacggag ggccacagaa atggcaccaa ctccgcagcg
     1561 ccgcccagga atctctggaa ccctatgttt tcgaggtcgc gccagcctga ctgattttat
     1621 ctgggcttgc gattgccgga ccgcagcaaa gcccagtgga aacaccgggc cttgtccagc
     1681 gcatcgcacg tgcgttcatt gcgataaccg attaaacatc tcgacgtcgc agtgaaccag
     1741 tcagcaccga aacgttcgca agattaccaa agtcatggat caacgccagc gtcgcctcga
     1801 tctggtcgtc gtgcatgccc gcctcgggaa gcagcgttcg aaatttgccg tcgatgtcgc
     1861 tccaggcgat tcccagggcg ccggcgccct tcggcaggta aaccgtatcg gttgatatac
     1921 ggccatcata ggtccggatc gccacggtgg cgccctggcg atagcggacc gtgttctcga
     1981 tcggcgggct gccaacctgg accttgtcga tcagcatgtg gatggcgggg tcgccgatct
     2041 tcgcggcact cgcatgtttc caggtgaatt cgtggtcagc cgcgccggcc gcggcgaaat
     2101 aggccaggct atgggccatc tcaatgagat cggttggatg ccgtggaccg cccagcacgg
     2161 tcaggcccgg gcgggaaacg gtgatgcttt ctacctcggg ggcggccatc ttgccacgtc
     2221 gcgcggcgtt ggcggcggct tcggcgaatg catggtaggg gtgcccgcca ggaacgagct
     2281 tgatggccag gtcggtgatg atgtcccagc tttgccc
//
LOCUS       DASWQN010000626         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1247699, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000626 DASWQN010000000
VERSION     DASWQN010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247699"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VGC82_10640"
     CDS             complement(<1..306)
                     /locus_tag="VGC82_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEY0423769.1"
                     /translation="MDANVKRNAPVRASPADRPLTAGGALAALGIVYGDLGTSPLYTF
                     QTIAGTVGGHPSTQDALGLLSLVVWALVITVSIKYCVFVMRADNHGEGGILALMALVT
                     "
     gene            complement(398..2419)
                     /locus_tag="VGC82_10645"
     CDS             complement(398..2419)
                     /locus_tag="VGC82_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0423770.1"
                     /translation="MAAPENLLGRETSPYLLQHADNPVHWRPWGKAALDEAKAANKPI
                     MVSIGYAACHWCHVMAHESFEDADTAALMNSLFVNIKIDREERPDIDHIYMSALHALG
                     EQGGWPLTMFTTPDGTPFWGGTYFPPEPRWGRPSFRQVLHGVADAFHANAAVVTQNTK
                     ALTQFLEQQATASVGEAPGPAQLEAVAGAYLKMTDPQHGGLRGAPKFPNPPIYRFLWQ
                     NAFRSGQTEGRDALHLMLERMSQGGIYDHLGGGFARYSTDDIWLVPHFEKMLYDNAQL
                     LELLALAHADRPNPLYAQRAAETVGWMMRDMSAQRIDGKAAFAASEDADSEGEEGRFY
                     VWTEAEVDALLGQDSGGFKRAYDVTPGGNWEGHTILRRIALFGSPDHEAALSRGREVL
                     FGAREKRVRPGRDDKVLADWNGLAIAALARCSMVFGKPEWLDRAREAFDFILTHMTAD
                     HGGVDHAWRLGRVTAPGLIEDQAAMTRAALALHEATGEAAFLGVAERLANAARDAFAD
                     GHGGFFTTAAGAADVPLTRPRTAADNATPAGNGIMAEALARLFHLTGNADWRERANGV
                     LHAFGGQTDQLVGMPTLLAAADLLEEGASVVITGTAPAALVKAALNSPDPAVAVICAG
                     GSGAVPTGHPAFGKTAGTHQAAAYVCRRNACGLPVMDASVLSQVLRTRV"
     gene            2546..>2887
                     /locus_tag="VGC82_10650"
     CDS             2546..>2887
                     /locus_tag="VGC82_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY0423771.1"
                     /translation="MFQPRHRGLTNHASGVAAENAACAALLADGWTIHARRLRTKAGE
                     VDVVAEKAGILAIVEVKRRPTLAEAASCLTVRQQCRLLGACEIVLAEHPDWGINGVRF
                     DVLVVNPAGQVR"
BASE COUNT          519 a         1005 c          879 g          484 t
ORIGIN      
        1 cgtcaccagt gccatcagcg ccaggatacc accctcgccg tggttgtccg cccgcatcac
       61 gaaaacgcag tatttgatcg acaccgtaat gacaagcgcc catacgacca gcgacaatag
      121 ccccagcgcg tcctgggtgg acggatgacc accgaccgtc ccggcgatgg tctggaaggt
      181 atagagcggg ctggtgccaa ggtcgccgta aacgatgcca agcgcggcca acgcgccccc
      241 ggcggtaagc ggccgatcgg caggcgaggc acgcaccggg gcatttcgtt tcacattcgc
      301 atccattgat ttctccgccg gagccgcccg ccccagaccg gccgggtcaa gcggggccat
      361 ctgcgcccga acgacgttcg gaggaacctt cccgcgatca aacccgcgtg cggagcacct
      421 gcgacagaac ggaggcatcc atgaccggca ggccacaagc gttacgccgg cacacataag
      481 ccgcggcctg gtgggttccg gcggtcttgc caaacgccgg gtgaccggtc ggtacagcgc
      541 cgctgccgcc ggcgcatatc accgccaccg cggggtccgg gctattgagg gcagccttga
      601 ccaatgcggc aggggcggta cccgtgatca cgacgctggc tccctcctcc agcaaatccg
      661 ccgctgccaa cagcgtgggc atcccgacta actggtccgt ctgtccgccg aacgcgtgca
      721 gtaccccatt cgcacgttcc cgccagtcgg cattgcccgt caggtgaaac agacgcgcca
      781 acgcctcggc cattatgccg ttgccggcgg gcgtggcgtt gtcggcggcg gttcgcggcc
      841 gggtcagcgg tacgtccgcc gccccagccg ccgtggtaaa gaacccacca tgcccatccg
      901 cgaaggcatc ccgggcagcg ttcgccagcc tttcagccac acctaaaaat gccgcttctc
      961 ccgtcgcctc atgcagagcc agcgccgccc gggtcatcgc cgcctgatcc tcgatcagac
     1021 caggagcggt aacgcggcca agacgccaag catgatcgac accgccatga tccgcggtca
     1081 tatgcgtcag aatgaaatcg aacgcttccc gcgcgcgatc cagccattcc ggtttaccga
     1141 ataccattga acaacgagcc agggcggcga tcgccaagcc gttccaatct gccaggacct
     1201 tgtcgtcgcg ccccggacgt acccgttttt cccttgcccc gaacagcacc tcccggcccc
     1261 gagacaaagc tgcctcgtgg tcgggagacc cgaacagagc gatccggcgc aagatggtat
     1321 ggccttccca gttgccgcct ggggtgacgt cataggctcg cttgaacccg cctgagtcct
     1381 gccccagcag ggcatccacc tcggcctcgg tccagacata gaagcgccct tcctcgccct
     1441 cgctgtcggc atcctcggaa gccgcgaacg ccgccttgcc gtcgatccgc tgcgcgctca
     1501 tgtcgcgcat catccagccc acggtctctg ccgcgcgctg tgcgtaaagc gggttcggcc
     1561 ggtcggcatg agcgagcgcc agaagctcca gcaactgcgc gttgtcgtag agcatcttct
     1621 cgaagtgcgg caccagccag atgtcgtcag tcgaataacg cgcgaagccg ccgccgaggt
     1681 gatcgtagat cccaccctgg gacatgcgtt ccagcatcag gtgcagcgcg tcccggcctt
     1741 ccgtctggcc gctgcgaaac gcgttctgcc acaggaagcg gtagatcggc ggattgggaa
     1801 acttcggcgc accgcgcaat ccgccatgct gcggatcggt catcttcaag tatgcccccg
     1861 cgaccgcctc cagttgcgcc ggtcccggtg cctctcccac cgaggccgtc gcctgctgtt
     1921 ccaggaactg cgtcagcgcc ttggtattct gtgtcaccac cgccgcattc gcgtggaagg
     1981 catccgcgac gccgtgcagc acctgccgga acgacggacg gccccatcgc ggttcgggcg
     2041 gaaaatacgt cccgccccag aacggagttc cgtcgggtgt ggtgaacatc gtcagcggcc
     2101 agccgccttg ctcgccgagt gcgtgcaacg ccgacatgta gatgtgatca atgtcgggac
     2161 gctcttcgcg gtcgatcttg atgttgacga acaggctgtt catcaacgcc gcggtgtccg
     2221 cgtcctcgaa ggattcgtgc gccatgacat ggcaccaatg gcatgcggca tagccgatgg
     2281 agaccatgat cggtttgttg gctgccttcg cctcgtcaag cgcggccttg ccccatggcc
     2341 gccagtgaac cgggttgtca gcatgctgga gcagataagg agaggtctcc cggcccagca
     2401 ggttttccgg tgcggccatg atgcggatcc cgacgaattg ccatccagac ataggtcgct
     2461 ggcgggcgat gccaactccg ccgaaccgga gagcgaaccg ggcaccatta accggatgct
     2521 aaccgaccga cactagtctg gctggatgtt ccagccgcgc caccgaggcc tgaccaacca
     2581 cgccagcggc gtcgccgccg agaatgccgc ctgtgccgct ctgctggccg acggctggac
     2641 catccatgct cgccgccttc gcaccaaagc cggggaagtc gatgtggtgg cggaaaaagc
     2701 cggcatcctg gcgatcgtcg aggtcaagcg caggccgacc ctggccgagg ccgcttcctg
     2761 cctgacggtt cgccaacagt gtcgcctgct tggtgcctgc gagatcgtcc tggccgaaca
     2821 tccggactgg ggcatcaatg gggttcgctt cgacgtcctc gtggtgaacc cggcgggaca
     2881 ggttcgc
//
LOCUS       DASWQN010000627         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_587908, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000627 DASWQN010000000
VERSION     DASWQN010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587908"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VGC82_10655"
     CDS             complement(<1..572)
                     /locus_tag="VGC82_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0423772.1"
                     /translation="MTGFDQPDWNGPPAETLDELYENAPCGYLTTRADGVIVRANATL
                     LRFLGESRDTLLGRPIQSILTTGARLFYETNCAPLLTLHGSLDEISLDLARADGGLLP
                     ALLNWRRVDDAQGRILGYRVMVVSAVERVAYERQLFAERERARQQEAMVRELNADLER
                     RVEARTAELVQAKKMEGLGQLTGGIAHDFN"
     gene            complement(576..1370)
                     /locus_tag="VGC82_10660"
     CDS             complement(576..1370)
                     /locus_tag="VGC82_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0423773.1"
                     /translation="MDVLRRNNVSVSGHGARTILFSHGFGCDLSAWRDVAPAFEDDWR
                     VVLFDHVGSGSSDITAYDPAKYASLLGYAQDVQEVMATLGLQGAVFVGHSVAAMIGML
                     AAIEQPRRFASLVMLCPSPCYLNDGDYIGGFSRADIGGLLDMLDRNFLAWSQTTAPAI
                     MGNPERPGLGQSLTESFCRMDPEIARGFARATFLSDHREDLSRLRIPSFVLQTRADII
                     APVAVGEYVAAHLPDSTFAMMRATGHCPHMSAPSETIERIRQYLAA"
     gene            complement(1544..>2542)
                     /locus_tag="VGC82_10665"
     CDS             complement(1544..>2542)
                     /locus_tag="VGC82_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423774.1"
                     /translation="IGIWGAVILTILRMLQGIGVGGEWGGSVLIAMEWSRTHQSRGLV
                     AAWPQFGVPAGLFLSNLVILAFSAWSGADFVAWGWRVPFMLSIVLIGIGLWIRLGILE
                     TPVFKQLVEGKKIEKAPIIEVIKRQPREIILSALLRMAEQAPFYIFTAFVFSYGTGTL
                     KMSRELVLSAVLVASCVSFVTIPLSGHISDRIGRRKMYLIGAAVTGVFGFLYFAMLAS
                     GSAALVFAAIVLSLIPHDMMYGPQAALIAEAFTPRLRYSGASLGYQLASVIAGGPAPL
                     IATALFAAYHTGYAVAIYIAACAVISLVSAAMMPDYTGKDISAEYDAPVGPVATRT"
BASE COUNT          506 a          814 c          799 g          423 t
ORIGIN      
        1 ttgttgaaat cgtgggcgat gccgccagtc agttggccca ggccttccat ctttttcgcc
       61 tggaccagtt cggccgtgcg ggcttcaacg cggcgttcca aatcggcgtt cagctcgcgc
      121 accatcgctt cctgctggcg ggcccgctcc cgttcggcga agagctggcg ttcgtaggca
      181 acccgttcga cggcgcttac caccatgaca cggtagccca ggatgcggcc ttgcgcatca
      241 tcgacacggc gccagttcag caacgccggc agcaacccac catcggctcg ggccagatcg
      301 agcgaaattt cgtccagcga gccgtgcagg gtcagcagcg gcgcgcagtt ggtctcgtag
      361 aacagacgcg cgccggtcgt caggatcgac tggatcggcc ggcccagcag ggtgtcgcgg
      421 ctttcgccga ggaaacgcag cagggtcgca ttggctctga cgatgacccc atcggcccgc
      481 gtcgtcagat aaccgcacgg agcgttttca tacagttcat cgagcgtttc ggctggcggc
      541 ccgttccaat ccggctgatc gaatcctgtc accggtcagg ccgccaggta ctggcggatg
      601 cgctcgatcg tctcagacgg ggcgctcatg tgcgggcagt gcccggtggc ccgcatcatc
      661 gcgaaggtgc tgtcgggtaa atgcgccgcg acgtattctc cgaccgctac cggggcgatg
      721 atgtccgccc gggtctgcag gacgaacgat gggatgcgca acctggacag gtcctcgcgg
      781 tggtccgaca ggaaggtcgc gcgtgcgaag ccgcgagcga tctcggggtc catgcggcag
      841 aaactttcgg tcagcgactg cccgaggccg gggcgctccg ggttgcccat gatagccggc
      901 gccgtcgttt gcgaccaggc gaggaaattc cggtccagca tgtcaagcaa gccaccaatg
      961 tccgcgcggc tgaatccgcc gatgtagtcg ccgtcgttca gatagcaggg cgacggacac
     1021 agcatgacca gggacgcgaa ccgtcgcggc tgttcgatcg ccgcgagcat cccgatcatg
     1081 gcggccacgg agtgcccgac gaacaccgcg ccctgtagcc caagcgtggc catgacctcc
     1141 tgcacatcct gcgcgtaacc cagcaggctg gcatatttgg ccggatcata ggccgtgatg
     1201 tcggaactgc cggatcccac atgatcgaac aacacgacgc gccaatcgtc ctcgaatgca
     1261 ggtgcgacgt ctcgccacgc gcttaggtcg cagccgaatc catgggaaaa caggatcgtt
     1321 ctggcaccgt ggccggaaac gctgacgttg ttacggcgta gaacgtccat tcctgcatcc
     1381 cggcccgagg gcagcgtcat aaggacgcac ccgacagtaa aagatatgcc gcgccggatc
     1441 caatcaggct cgcggaaatc acggagtttc gtcgcgtgac attcgatgtg acggtatttc
     1501 cacggcaaca tcaagacttc gatgcaagga acccgcgggt cgttcaagtc cgtgttgcca
     1561 ctggtcccac tggcgcatca tattcggcgg aaatgtcctt gccggtgtag tcgggcatca
     1621 tggcggctga aaccaggctg atgaccgcac aggccgcgat gtagatcgcg acagcgtacc
     1681 cggtgtgata ggcggcgaac aacgcggtcg cgatcagcgg cgcgggaccg ccggcgatca
     1741 ccgacgccag ttggtagccc aatgaggcac cgctgtagcg cagccggggc gtgaatgcct
     1801 cagcgatcag cgcggcttgc ggcccataca tcatgtcgtg cgggatcagc gacagcacga
     1861 tcgcggcgaa cacaagcgcc gcggagccgc tggcgagcat cgcgaaataa aggaagccga
     1921 aaacccctgt cacggccgcg ccgatcaggt acatcttgcg ccggccgatc cggtcggaga
     1981 tgtgacctga cagcgggatg gtgacgaacg acacgcagga tgccaccagc accgccgaaa
     2041 gcaccagttc ccgcgacatc ttcagggtgc ccgtgccgta ggaaaacacg aacgccgtga
     2101 agatgtagaa cggtgcctgt tcggccatgc gcagcagcgc ggacaggatg atctcccgcg
     2161 gctggcgctt gataacctcg atgatcggcg ctttctcgat cttcttgccc tccacgagct
     2221 gcttgaagac cggggtttcc aggatgccga ggcggatcca caggccgatg ccgatcagca
     2281 cgatgctcag catgaacggc acgcgccagc cccaggcgac aaaatccgct ccagaccacg
     2341 cactgaatgc caatatcacc agatttgaga gaaacaagcc ggcgggcacg ccgaactgtg
     2401 gccaggcagc gaccaggccg cggctttggt gggtgcgcga ccattccatc gcgatcagca
     2461 ccgatccgcc ccattcgccg cccaccccaa tgccttgcag catgcgcagg atggtgagga
     2521 tcaccgcacc ccagatgccg at
//
LOCUS       DASWQN010000628         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1510960, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000628 DASWQN010000000
VERSION     DASWQN010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510960"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VGC82_10670"
     CDS             complement(<1..329)
                     /locus_tag="VGC82_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0423775.1"
                     /translation="MVTQTDVQGWAEGLDGVVERIAPRFGRAEPRRRARAYMRGLLAP
                     IERKNGWQLAEAVGDATPDGVQDFLSRVHWDADAVRDDLQAYVVQHLSDPDGVAVLDE
                     TGFLKKG"
     gene            complement(377..1063)
                     /locus_tag="VGC82_10675"
     CDS             complement(377..1063)
                     /locus_tag="VGC82_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0423776.1"
                     /translation="MADYVISPPAIPSVPVAGGGSFPVRRVFCVGRNYAEHAREMGSD
                     PDREPPFFFMKPADALLLNNADMPYPSQSTQLHHEMELVVAIGEGGTNIKEADALRHV
                     WGYAAGLDMTRRDLQNAAKKEGKPWDMGKGFDYSAPIGLMVPAAKIGHPAAGLIELKV
                     NGRVRQTSDLSKMIWNVPETIAYLSRLVKLAPGDLIYTGTPENVAAVERGDLLEGVVE
                     GVGSVTTRIV"
     gene            1140..2432
                     /gene="dctA"
                     /locus_tag="VGC82_10680"
     CDS             1140..2432
                     /gene="dctA"
                     /locus_tag="VGC82_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HEY0423777.1"
                     /translation="MAGTSARSAKGLYIQVLLGVVIGALLGHFWPALAVQMQPFGDAF
                     IKLVRMIIAPIVFVTVVVGIAKLSDAKEVGRIGIKAILYFEVMTTIAMFIGLIVAHVF
                     QPGAGLNIDPATLDSKAVARYADAPHQDVTTFLLNIIPNTVIDAFAKGEILQVLLFAV
                     LFGLGLSRLGERGAAVVRVLDEAGAALFGVIALIMRVAPLGAFGAMAFTIGRYGIGAL
                     AQLGFLMLCFYLTCAIFVFVVMGAVAAAMGLNILKILRFVKEEFLIVLGTSSSEAAMP
                     TLMEKLELLGCGKSLVGLTVPLGYAFNLDGSSIYFTMAIAFIAQALNIALSWSDYLLI
                     LAVLLLTSKGAATVTGGGFITLAATLATMNGKVPVAGMVLVLGIDRFMSEARAITNLF
                     GNTVATIFVAWWEGSLDLQRTRAILDRSGGVEDAVLAE"
BASE COUNT          435 a          836 c          830 g          528 t
ORIGIN      
        1 ttgccctttt tcaggaagcc ggtttcatcc agcacggcca cgccatcggg atcgctcaga
       61 tgctgcacga cataggcttg taggtcatcg cgcaccgcat cggcgtccca gtgcacccgg
      121 ctcaggaagt cctgcacccc atccggggtc gcatcgccta ccgcttcggc caattgccag
      181 ccgttcttgc gctctatcgg cgccagcaaa ccgcgcatgt aggctcgagc ccgccgccgt
      241 ggctcggccc gcccaaaccg cggcgcaatc cgttcaacaa cgccgtccag gccttcagcc
      301 cagccttgca catccgtttg cgttaccatg ccgcgttacc tcctagtcga ggtaacgctc
      361 aaacccggct gtagtactag acgatgcgcg tggtcaccga accaacgcct tcgaccacgc
      421 cttccagcaa gtcgccgcgt tcgacggcag ccacgttctc tggcgtgccg gtgtagatca
      481 gatcgcccgg tgccagcttc accagtctgg acaggtaggc gatcgtctcc ggcacgttcc
      541 agatcatctt cgacaggtcc gaggtctggc ggacgcggcc gttgaccttc agctcgatca
      601 ggccggcggc ggggtgtccg atcttcgcgg ccggaaccat cagtccgatc ggcgccgaat
      661 agtcgaaccc cttgcccatg tcccagggct tgccttcctt cttggccgcg ttctgcaggt
      721 cgcgccgtgt catgtccagg cccgccgcgt agccccagac gtgcctcagt gcgtcggctt
      781 ccttgatgtt cgtgccgcct tcgccgatcg ccacgaccag ctccatttca tgatgaagct
      841 gggtgctttg cgacgggtac ggcatgtcgg cgttattgag cagcagggca tcggccggct
      901 tcataaagaa gaaaggaggc tcccggtccg ggtcgctgcc catctcccgg gcgtgttcgg
      961 catagttgcg gccgacacaa aaaacccgcc taacgggaaa actaccgccg cccgccacgg
     1021 ggacggatgg tatcgccgga ggggagatga cgtaatcagc cattcggttg caccttcagg
     1081 gtgtgaaagc ttcggtcgga atcagcggcc ggacataagg gaaagaggaa caccaggcaa
     1141 tggcaggcac cagcgcacgc tccgccaaag gtctttatat ccaggttctt ctcggcgttg
     1201 tcatcggcgc cttgctgggc cacttctggc cggcgttggc ggtgcagatg cagcctttcg
     1261 gcgacgcgtt catcaaactg gtgcgcatga tcatcgcccc gatcgtcttc gtgacggtgg
     1321 tggtcgggat cgccaagctg tccgacgcga aggaagtcgg ccggatcggc atcaaggcaa
     1381 tcctttactt cgaggtgatg acgacgatcg ccatgttcat cggcctgatc gtcgcccatg
     1441 tgttccagcc gggtgccggc ctcaacatcg atccggcgac cttggacagc aaggccgtgg
     1501 cccgctacgc cgatgcgccg catcaggatg tcaccacctt cctgctgaat atcatcccca
     1561 acaccgtgat cgacgcgttc gccaagggcg agatcctgca ggtgctgctg ttcgccgtgc
     1621 tgttcggcct gggcctgtcg cgcctgggtg agcgcggtgc ggccgtcgtg cgtgtcctgg
     1681 acgaagccgg agccgcattg ttcggcgtca tcgcccttat catgcgggtg gcgccgctgg
     1741 gcgcgttcgg tgccatggcg ttcaccatcg gccgctacgg catcggcgca ttggcccaac
     1801 tcgggttcct gatgctgtgt ttctacctga cctgcgccat cttcgtgttc gtggtgatgg
     1861 gcgcggtggc tgccgcgatg gggctgaata tcctgaagat cctgcgtttc gtgaaagagg
     1921 agttcctgat cgtgctgggc acttcgtcgt ccgaggcggc gatgcccacg ctgatggaaa
     1981 agctggagct gctgggctgc ggcaagtccc tggtcggact gaccgtgccg ctgggatatg
     2041 cgttcaacct ggacggatcg tcgatctact tcaccatggc gatcgcgttc atcgcccagg
     2101 cgttgaacat cgcactgagc tggagcgact atttgctgat cctggcggtg ctgctgctga
     2161 cgtccaaggg tgcggcgacg gtgaccggcg gcgggttcat caccctggcg gcgacgctgg
     2221 ccaccatgaa cggcaaagtc cccgtggccg gcatggtcct ggtgctcggg atcgaccgtt
     2281 tcatgagcga ggcgcgcgcc atcaccaacc tgttcggcaa cacggtcgcg acgatcttcg
     2341 tcgcgtggtg ggagggttcg ctggatcttc aacggacgcg ggcgatactg gatcggagcg
     2401 gcggggtgga ggacgcggtg ctggcggagt aggcaggtcg agcgtgctaa gcatcacccg
     2461 tctcggagcg ggttatctat catccagccc caagcctgag ccgggttaca ccatagaagc
     2521 aaacgcgccg ccggacacag gccaagtccc tcggcacaca ggttttggcg gttgccgggc
     2581 ggcctctatg tggctctggc atcccggata tcgctgcgcg gacgtcagc
//
LOCUS       DASWQN010000629         4395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1247836, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000629 DASWQN010000000
VERSION     DASWQN010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247836"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..2106
                     /locus_tag="VGC82_10685"
     CDS             388..2106
                     /locus_tag="VGC82_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0423778.1"
                     /translation="MPRPIPALCIAFLLAWPAQPHAQPEAAPDEGFSPLDEITPANVK
                     QLVAAFTFRTGKPRAHTSAPLAVGDVLLVLTPFPHTLFGLDLTQPNAPVKWTYAPRPN
                     HLAEGLTCCGAPTGGIAAQDGRIYLNSLDGQVSALAAADGKVLWNATVAHPEAGEILA
                     TAPLPVMDELIVGTSGDDAGARGALIALDASSGGQRWKVFSTGPDNEAGIGESFHPFY
                     RTGDGPDLGVTTWPPAAWQQGGGGLAGPLVFDPGSGMLFQQTGHPAPLNPDQREGDNL
                     WTSGLFARDARTGAATWFDAIDPHDVHSLGAGGGIILATSQGRPILIHPDANGYLYEL
                     DRTSGEILSANPYLPVTATHGVDRATGRPVRDANFTLTRGSTTRDICPGWPAGSNALP
                     AFSALSGFAYLPVAQLCMDMQPVSANYIAGTPFAGANVRMKPAAGRSLGALIGWDIAA
                     RHAAWTLPESLPLRGGALVTGGGLVFYGTLDGFIKAADARTGTALWQFHTASGIVARP
                     ISYRAPDGHQYLAVLAGSGGLTGTNSAQEIDARDATAAHGLAAALGPLPTPDDASGVL
                     YVFRLP"
     gene            2103..2642
                     /locus_tag="VGC82_10690"
     CDS             2103..2642
                     /locus_tag="VGC82_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0423779.1"
                     /translation="MIALRTAVALVLLVAACKREERSFRTDPPIAQALDQVAVMPGGI
                     AGASPDVDRAQGHAFVGNAYQLSQGKQLFDWFNCSGCHANGGGASGPALMNAWWRYGP
                     DEVSIFVSIRDGRPHGMPAFRDKLTTEQVWQLTQYVRTIGTTSATTAAPNRNDSMHTR
                     PAENRGPATFDASKPAAGQ"
     gene            2645..3700
                     /gene="coxB"
                     /locus_tag="VGC82_10695"
     CDS             2645..3700
                     /gene="coxB"
                     /locus_tag="VGC82_10695"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY0423780.1"
                     /translation="MRRSTMRVATALPLLCAGCSGWQSAMDPGGPQAAKLKALIWVFL
                     ITCGIIWLLVMLVLAAAVWHRRPPADPLANPLANPLADPATNPSADPLATPNRTASIV
                     VGAAVAASVVVIFGLTLASYFATRGIFDSRPEPLIIQVRGHQWWWEATYKDRSINQNF
                     LTANELHVPVGQPIRIELAADDVIHSFWAPNLTGKQDLIPGRDNALTFTVDKPGTYRQ
                     QCAEFCGLQHAHMAMLIVAEPAEAYQNWRHAQLVEPAAPTEAAEQRGRQAFLAKPCAA
                     CHTIRGTQASGTLGPDLTHVGSRQYIVAGILPATRGSLAAWIADPQTIKPGNNMPMVP
                     LTADELNDLCAYLASLK"
     gene            3697..>4395
                     /locus_tag="VGC82_10700"
     CDS             3697..>4395
                     /locus_tag="VGC82_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0423781.1"
                     /translation="MNAGDISPDVRDAAIPQDVLEAQLGTIWRTERGLLGWLGTVDHK
                     LIGRRYIATAFVFLALGGILAMLMRVQLSGPENRLIGPDRYNQIFTMHGANMMFLFAV
                     PVMEAVAVYVVPLMIGTRNIAFPRLNAFSYWMYLAGGILLWTAFALNMGPDVGWFAYV
                     PLSGPQYGIGKRSDIWAQMITFTEVSALAVAVEIVVTVLTQRAPGMSLDRIPLFVWSM
                     LVTAFLIILAMPSIM"
BASE COUNT          763 a         1500 c         1381 g          751 t
ORIGIN      
        1 gcacctgaag cgggtctttt gaagaaggca cttagttggg gagcaaagga tgacacaatc
       61 gattcatctg caagattccg acccggcaat gtcgaacccg ggggatgagg tgccacccgg
      121 cacacccggg tccggtgaag atgtctgtcc aaaatgccat ggctccggac gggaagaggg
      181 tcaaacctgc cagaattgca acgggaccgg cgtgatcacc agggcaatcg gcggagcgtg
      241 aacgcatccg aaagggaagc gccggggcgc gtttgatata gcggccgccg cggttgttgc
      301 taacaatcac accgtcgaag tccaatgcgt cagcccacga tgattgagag ccccaaggcc
      361 ttccgttcgc atgaccgagg ccaggcgatg ccgcgcccga tcccagccct atgcatcgcc
      421 ttccttttgg cctggccggc tcaaccccac gcccagcccg aggcagcccc cgacgagggg
      481 ttcagtccgc ttgacgagat cacgcctgcg aacgtcaaac agctggtagc cgctttcacg
      541 ttcaggacgg gcaagccaag ggcccatacc agcgctcccc tggcggtggg agacgtcctg
      601 ctcgtgctta cgccgttccc gcacacgctg tttggcctgg acctcaccca gccgaacgcg
      661 ccggtgaaat ggacctatgc gccgcggccc aaccatctgg ccgaaggctt gacctgctgc
      721 ggtgccccca ccggcgggat cgctgcccag gacggtcgta tttatttgaa cagcctggat
      781 ggacaagtaa gcgcgctcgc cgcggcagac ggcaaagtcc tctggaacgc cacggtggcg
      841 catccggagg ccggcgaaat cctggcgacg gcgcccctgc cggtcatgga cgaattgatc
      901 gtcggcacct cgggcgacga tgccggcgcc cgaggcgcgc tcatcgcact ggacgcctcc
      961 agcggcggtc agcgctggaa agtgttcagc accggccccg acaacgaggc cggcatcgga
     1021 gaaagcttcc atccgttcta caggaccggc gacggcccgg atctcggtgt caccacatgg
     1081 ccgcccgcgg cctggcaaca gggcggtggc ggcctcgccg gcccgctggt gttcgatccc
     1141 ggctcgggca tgctgttcca gcagaccggc catcccgccc ctctgaaccc cgaccagcgg
     1201 gagggcgaca acctatggac gtcgggcctg ttcgcgcgtg atgctcggac cggtgccgcg
     1261 acctggttcg atgcgattga tccacacgac gttcacagcc tgggagccgg cggtggtatc
     1321 atcctggcaa ccagccaggg ccgtcccatc cttatccatc cggatgcgaa tgggtatcta
     1381 tacgagctgg atcgtacaag cggcgagatc ctctcggcca atccctatct gcccgtcacc
     1441 gcgacgcacg gggtcgatcg cgcaaccggc cgccctgtcc gcgatgcgaa tttcactttg
     1501 acccgaggct ccacgacccg cgatatctgt cccggctggc ctgccggatc gaacgcgctc
     1561 ccggcgttct ccgcgctgag cggcttcgcc tatctgccgg ttgcccaact ctgcatggac
     1621 atgcagccgg tctcggccaa ctacatcgcc ggaaccccat tcgcgggcgc caacgtgcgc
     1681 atgaagccgg cggcgggccg atccctgggc gcactgatcg ggtgggacat cgcggcgagg
     1741 catgcggcct ggacactgcc ggaatcctta ccgttgcgcg gcggagcgct cgtgacaggc
     1801 ggcggcctgg tcttctatgg aacgttggac ggcttcatca aagccgccga tgcccggacg
     1861 ggaaccgcgc tttggcaatt ccataccgcc tccggcatcg tcgcacgccc catcagctac
     1921 cgcgcgccgg acgggcacca atacctcgct gtcctggccg gttcgggcgg actgaccggc
     1981 accaattcag cgcaagagat cgacgcccgc gacgccacgg ccgcgcatgg gctggcggcg
     2041 gcactgggac cgctgcccac gcccgacgac gcaagcggcg tcctttacgt attcaggctg
     2101 ccatgatcgc gctgcggaca gcggtcgcgc tcgtgctcct ggtcgcagcc tgcaagcggg
     2161 aggagcggag cttcaggacc gacccgccga tcgcccaggc tctggaccag gtcgcggtca
     2221 tgccgggggg catcgccggc gcctcgccgg atgtcgatcg cgcccaaggc cacgcctttg
     2281 tcggcaacgc ctatcagctc agccagggca agcagttgtt cgactggttc aactgcagcg
     2341 gctgtcatgc caacggcggc ggcgcttcgg gtccggcact gatgaatgct tggtggcgct
     2401 atggaccgga cgaggtctcc atcttcgtct cgatccgcga cgggcggccg catgggatgc
     2461 cggcgtttcg cgacaagctg acgacggagc aggtctggca actcacccag tatgtccgga
     2521 ccattggaac cacatcggcg acgaccgctg cgccgaatcg caacgactcg atgcacaccc
     2581 ggccggcgga gaaccgtggt ccggcaacct tcgacgccag caaaccggcc gccggccaat
     2641 gacgatgcgt cgatcgacga tgcgggtcgc cacggcgctg ccgctgctct gtgccggctg
     2701 cagcggctgg caatcggcga tggaccccgg cggtccgcag gccgcgaagc tgaaggcgct
     2761 gatctgggtc ttcctcatca cctgcggcat tatctggctc ctggtcatgc tggtgctggc
     2821 ggccgcggta tggcaccgcc gcccacccgc cgatccactg gccaatccac tggccaatcc
     2881 attggccgac cctgcgacta atccatcggc cgatccattg gccacgccca accggacggc
     2941 atccatcgtg gttggcgctg ccgtcgcggc cagcgtcgtg gtgatcttcg gccttacgct
     3001 cgccagctac ttcgcgacgc gtggcatctt cgacagccgt ccggagccat tgatcatcca
     3061 ggtccgcggc caccagtggt ggtgggaggc gacctacaag gacaggtcca taaaccagaa
     3121 tttcctcacc gcgaacgagc tgcacgtccc ggtgggccaa cccatccgca tcgaactcgc
     3181 ggcggacgat gtcatccatt ccttctgggc gcccaacctc accggcaagc aggatctgat
     3241 ccccgggcga gacaacgcac tgaccttcac cgtcgataag cccggcacct atcgccagca
     3301 atgcgccgaa ttttgcggcc tgcagcacgc gcatatggcc atgctgatcg tagccgaacc
     3361 ggctgaagcg tatcagaact ggcggcacgc ccagctcgtc gaacccgctg ccccgaccga
     3421 ggccgctgag cagcgaggcc ggcaggcctt cctggccaag ccctgcgccg cctgccacac
     3481 gatccgcggc acccaggcgt ccggcacgct gggtccggac ctgacacacg taggatctcg
     3541 ccaatacatc gtagcgggca tcttgccggc gacccgaggc tcgttggccg cctggatcgc
     3601 cgacccgcag acgataaaac cgggcaacaa catgccgatg gtgccgttga cggccgacga
     3661 actgaacgac ctttgcgcct acctggcatc gttgaaatga acgcgggcga catctccccc
     3721 gacgtgcgtg atgccgcgat cccgcaggac gtgctggaag cgcagctcgg gacgatctgg
     3781 cgcaccgagc gggggctgct gggatggctg ggcaccgtgg atcacaagct gatcggccgc
     3841 cgctacatcg cgactgcgtt cgtcttcctg gcactgggcg gaatacttgc gatgctgatg
     3901 cgcgtgcagc tttcggggcc ggagaaccgc ctgatcggcc ctgaccggta caaccagatc
     3961 ttcaccatgc acggcgcgaa catgatgttc ctgttcgcgg tgccggtgat ggaggcagtc
     4021 gcggtgtatg tcgtgccgtt gatgatcggc acgcgcaaca tcgcctttcc ccggctgaac
     4081 gccttttcct actggatgta cttggcgggc ggtatcctgc tgtggacggc cttcgcgctg
     4141 aacatgggac ccgatgtagg ctggtttgcc tacgttccac tgtccgggcc gcaatacggc
     4201 atcggcaagc gttcggatat ctgggcgcag atgatcacct tcaccgaagt ctctgccctc
     4261 gccgtggcgg tcgagatcgt tgtcacggtg ctgacgcagc gcgcccctgg catgtcgctc
     4321 gatcggattc cgctgttcgt ctggtcgatg ctggtgaccg ccttcctgat catcctggcg
     4381 atgccctcga tcatg
//
LOCUS       DASWQN010000630         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1511072, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000630 DASWQN010000000
VERSION     DASWQN010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511072"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..404)
                     /locus_tag="VGC82_10705"
     CDS             complement(105..404)
                     /locus_tag="VGC82_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HEY0423782.1"
                     /translation="MPDVPPDRLSNNPKSPHYDEAALRRGIGIRFKGVERTNVEEYCV
                     SEGWIRVAVGKTLDRRGNPLTMKAVGEVEPYFRDVQPDTEQQGAAKPDDGNPEAL"
     gene            complement(490..1380)
                     /locus_tag="VGC82_10710"
     CDS             complement(490..1380)
                     /locus_tag="VGC82_10710"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0423783.1"
                     /translation="MPTQPVSFYSEGTRLAGDLFLPDGLASGEKRGGIVLCHGYTGVR
                     NLYLPDTAKALTDAGYIVLTFDYKGWGDSDGPRSRLSPYGRVIDSQAAITFLGAQPNV
                     DADRLGIYGTSYGGATVVWTAAVDPRVKAVVAVVGIGHGRRWMRSVRRPDEFADLLAR
                     SEADRVRRVMTGKSEFADRNAVLLPDRQSADLAAAARRGNPGAVGEIPLEFIDDTLGF
                     HPEWIVDKIAPRPVLFITSDDDRLVPPDESESLYAKAGEPKKLIVLKGWGHYEVYAGE
                     AFRQVIEPTLDWYGTYLPPR"
     gene            1439..2128
                     /locus_tag="VGC82_10715"
     CDS             1439..2128
                     /locus_tag="VGC82_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0423784.1"
                     /translation="MATYVLLHGAYQGGWIWQRVAPHLRAAGNRVFAPTLDGCAERRH
                     ALRPGIDTESQAAEISEMLFFEDLHDVVLVGTSCGGMVAARVAETSRERIGRIVLADA
                     LALFNGEAVSDHVKRAGAVTDGVATGPTPEDAANRMFASLNEPTKAWALARYTPHPVA
                     AMAAPVKLDKFWQQEWKASVIWCRQSVNPPVAHQKRAADTLKARWYELDTGHYPMLTE
                     PVALARLIMAE"
BASE COUNT          369 a          645 c          746 g          372 t
ORIGIN      
        1 gacggggtag ggatcggcca ggaaggcggg atcccggacg tcgaaggacg gttgggctgt
       61 cgcggacatc ttcaggctcc ggtcactgtt ccagcaacgg tgtatcacag cgcctcgggg
      121 ttcccgtcat cgggtttggc ggctccttgc tgctcggtat caggctgtac gtcgcggaaa
      181 tacggctcga cctcaccgac cgccttcatc gtgagtggat tgccgcggcg gtcgagcgtc
      241 ttgccgacgg ccacacgaat ccatccttcg ctgacgcagt attcctcgac attggtcctt
      301 tcgacacctt tgaagcggat gccgatgccg cgccgcaggg cagcttcgtc gtagtgtgga
      361 cttttcgggt tgttggacag gcgatcgggg ggaacgtccg gcatagggca ccttgaggca
      421 ataagacgca gcccgatacc gggtcatggc aagcctgtcc atgcaggcac cggggcggag
      481 agcgttcggc taccggggcg gcaggtaggt tccgtaccag tcaagggttg gctcgatgac
      541 ctggcggaac gcctcgccag cgtaaacctc ataatgtccc cagcctttca ggacgatcag
      601 cttcttcggc tcgccggcct tggcgtaaag ggattcgctt tcgtcgggcg gcaccaggcg
      661 atcgtcatcg ctggtgatga acaacaccgg ccggggtgcg atcttgtcca cgatccactc
      721 gggatgaaaa cccagcgtgt cgtcgatgaa ctcaagcggg atctcgccga ccgcgccggg
      781 gttgccccgt ctggccgcgg cagcgagatc ggcggactgg cgatccggga gaagcaccgc
      841 gttgcggtcg gcgaactcgg acttcccggt catgacgcgg cggacgcgat cggcttccga
      901 tcgggcgagc aggtcggcga actcgtctgg gcggcgaacg ctgcgcatcc agcggcggcc
      961 atggccgatg ccgacgaccg caacgaccgc cttgacgcgt ggatcgaccg cggccgtcca
     1021 gacgacggtg gcaccgccgt agctggtgcc gtagatacca aggcggtccg catccacgtt
     1081 cggctgcgcg ccgaggaacg tgatggcagc ctgcgagtcg atgacccggc catagggcga
     1141 caggcgcgat ctcggcccgt cgctgtcacc ccagccttta taatcgaacg tcagcacgat
     1201 gtagccagcg tcggtcagcg ccttcgcggt atccggcaga taaaggttcc gcacaccggt
     1261 atagccatga cacaggacga tgccgccgcg cttttcaccc gatgccagtc cgtcgggcag
     1321 gaacaggtcg ccggcgagcc gcgtgccctc gctgtagaaa ctgaccggct gggtcggcat
     1381 ggcttttccc ccattgctgt gctgatgttc gcggcagaat gagggggaga gaacgatcat
     1441 ggcaacctat gtgctgctgc acggcgccta tcagggcgga tggatctggc agcgtgtggc
     1501 gccgcacctg cgcgccgccg gcaaccgggt gttcgcgccg acgctggatg gctgcgccga
     1561 acgccggcac gccctgcggc ctggcatcga taccgaatcc caggcggcgg aaatctcgga
     1621 aatgctgttc ttcgaggatc tgcacgacgt cgtcctggtc ggaacaagct gcggcgggat
     1681 ggtggctgcc cgggtggcgg aaacgtcacg cgaacggatc ggccggatcg tgttggccga
     1741 cgcgctggcg ctgttcaacg gcgaggcggt gtccgatcac gtcaagcggg caggagcggt
     1801 gaccgatggc gttgcgaccg gcccgacgcc ggaggatgcc gctaaccgga tgttcgcctc
     1861 attgaacgag ccgacgaagg cctgggcgct ggcgcgatat acgccgcatc cggtggccgc
     1921 gatggcggcg ccggtgaagc tggacaagtt ctggcagcaa gagtggaaag cctcggtgat
     1981 ctggtgccgg cagagcgtca atccgccggt ggcgcaccag aagcgggccg cggacacgtt
     2041 gaaggctcgg tggtatgagt tggataccgg gcactatccg atgctgacgg aaccggtggc
     2101 gttggcgcgg ttgatcatgg cggaataacc gg
//
LOCUS       DASWQN010000631         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_455981, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000631 DASWQN010000000
VERSION     DASWQN010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455981"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..>1674)
                     /locus_tag="VGC82_10720"
     CDS             complement(10..>1674)
                     /locus_tag="VGC82_10720"
                     /inference="COORDINATES: protein motif:HMM:NF027658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HEY0423785.1"
                     /translation="PHDVVAYVDMTGEWAVNHPHERVFWKLHRAGGMRIAAFRSEHQH
                     VLAQSGDHRRIEWGTAFLAVAESAGDVIMGDIDHCRDTFARAGRLSGDHLRASQPCKV
                     DYISDPVAAAMLHLPIDGGVRHVLIAYDDEWSIELMQQRLRPWWRRHEGADPQAMLAA
                     AMHEADAVLARCDAFDAALATRAEAIGGLGYADLLGLTWRHAIAAHKLVAGPDGRPLF
                     FSKENFSNGCIATVDVTYPSAPLFLLFNPALLQAMLDPVIAYCASPDWPHPFPAHDLG
                     TYPIANGQTYGDWESGRGPHEDIVETQMPVEEAGNMLILIAALARAEGAPGYAGRHWP
                     LLRQWADYLIEAGFDPDEQFCTDDFSGTLGHNVNLSAKAIMGIACFAQLAAALGHHAD
                     AKRYRATAEAFARLWLERARDGEGTRLAFDQAGSWSLKYNLVWDRLLRLELFPEEELR
                     REQAFYRGKAGLYGVPLDGRGTLTKPEWMLWAACLADDPALLRDWVGCILRYANETPN
                     RVPFSDLYFTDSGRKMAFQARSVVGGFHIALLADAWTKGRLASQPS"
BASE COUNT          302 a          549 c          544 g          279 t
ORIGIN      
        1 aagattgatt caggaaggct gggaagcaag ccgacccttg gtccaggcgt ctgccagaag
       61 cgcgatgtgg aaccctccga ccacggaacg cgcctggaaa gccattttgc ggccgctgtc
      121 agtgaaatac aggtcggaga acggcacccg gttcggtgtt tcattggcgt aacgcaagat
      181 gcagccgacc cagtcacgca gcaacgccgg gtcatcagcc aggcaggcag cccatagcat
      241 ccattcaggc ttggtcagcg ttcccctgcc atccagcggt acgccgtaca gcccggcttt
      301 tccgcgatag aatgcctgct cccgtcgcag ttcttcctct ggaaacagct cgaggcggag
      361 cagccggtcc cacaccaggt tgtacttcag gctccagctt cccgcttggt cgaaggcaag
      421 gcgtgtcccc tcgccgtcgc gggcgcgctc aagccagagg cgggcaaacg cctcggccgt
      481 ggcacggtag cgtttggcat cggcatgatg tccgagcgcc gccgcgagtt gtgcgaagca
      541 agcaatcccc atgatggcct tagccgacag gttcacgtta tggcccagcg ttccagagaa
      601 atcgtcggtg caaaactgct cgtccgggtc gaatcccgcc tcgatgagat aatccgccca
      661 ttgccgcagc aatggccagt gccgtccggc ataaccgggt gcgccctcgg cgcgggcgag
      721 cgcggcgatc aggatcagca tgttgccggc ctcctcgacc ggcatctggg tctcgacgat
      781 gtcctcgtgc ggtccacgcc ccgactccca gtcgccgtac gtctgtccat tggcgatcgg
      841 gtaggtgccg aggtcgtgcg cggggaaagg atgtggccag tccggcgacg cgcagtatgc
      901 gatcaccggg tccagcatgg cctggagcag cgccgggttg aacagaagga acagcggcgc
      961 cgaagggtag gtcacatcca ccgtggcgat gcagccgttc gagaagttct ccttcgagaa
     1021 aaacagcggt cgcccgtcgg gcccggcgac gagcttatgc gccgcaatcg cgtgccgcca
     1081 agtcagcccc agcaggtcgg catagccgag gccgcctata gcttccgccc gcgtcgccaa
     1141 tgccgcatcg aacgcatcgc atcgcgccag cacggcatcg gcctcgtgca tcgcggctgc
     1201 gagcattgcc tgcggatcgg cgccttcgtg ccggcgccac caggggcgca ggcgttgctg
     1261 catcaactcg atcgaccact cgtcgtcata ggcgatcagc acgtggcgga cgccgccgtc
     1321 gatgggcagg tgcagcatgg cggccgcgac cgggtcggag atgtaatcca ccttgcacgg
     1381 ctgcgacgca cggaggtgat cgccgctgag ccggcccgcg cgggcgaaag tgtcgcggca
     1441 gtggtcgatg tcgcccatga tcacgtcgcc ggcgctttca gcgacggcca ggaaggcagt
     1501 gccccactcg atacggcgat ggtcgccgga ttgggccagg acgtgctggt gctctgagcg
     1561 gaacgccgct atgcgcatcc ccccggcgcg atgaagcttc cagaacacgc gctcatgcgg
     1621 atggttgacc gcccattcgc ccgtcatgtc gacataggcc acgacatcgt gcgg
//
LOCUS       DASWQN010000632         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_456380, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000632 DASWQN010000000
VERSION     DASWQN010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456380"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VGC82_10725"
     CDS             <1..426
                     /locus_tag="VGC82_10725"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEY0423786.1"
                     /translation="VLERLMGRWLSDAGDWTEEADLATALNQAAARILAVALTPEAIA
                     LHRLLIAESARFPELPLMLGQAGAGEGTSRIQAILSRAVAAGRLPPGDMAFAAEQFLH
                     LLLAGPQRRALGLGSKLDEHQTLVWRETSVALFLSGIER"
     gene            493..1569
                     /gene="rfbB"
                     /locus_tag="VGC82_10730"
     CDS             493..1569
                     /gene="rfbB"
                     /locus_tag="VGC82_10730"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY0423787.1"
                     /translation="MKLLLTGGCGFIGSAVVRHLLRQTDHAVVNVDKMTYAASEDALE
                     DCHRHNRHILVKADIADPASMRQVFEAHDPDAVMHLAAESHVDRSIDGPADFVQTNVV
                     GTFILLEASRRHYNNLSSDRKAMFRFHHISTDEVFGALEYGDPPFTETTPYDPRSPYS
                     ATKAASDHLVRAWSHTYKLPTIVSNTCNNYGPWQFPEKLIPLVTLNALEGKELPVYGD
                     GSNMRDWLFVEDHAEALVRIVEQGIPGETYAIGGRQPRSNLQVVKAICAHLDKRIPVP
                     NGPRERLIRYVTDRPGHDFRYEIDPSRSEQALRWKAPHDFETGLARTIDWYLDNRTWW
                     EALRAGRYAGQRLGTSTDSKEKAA"
     gene            1566..>2117
                     /locus_tag="VGC82_10735"
     CDS             1566..>2117
                     /locus_tag="VGC82_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY0423788.1"
                     /translation="MKGILLAGGSGTRLHPMTLASSKQLLPVYDKPMVYYPLSTLMLA
                     GIRDILLISTPEDLPQFKRLLGNGSRFGVRFQYAEQPKPDGIAQAFLIGRDWLAGEAC
                     ALALGDNLIHADHLSTLLRTAAARPVGATVFAYQVRDPERYGVVSFDAAGRVVDIVEK
                     PTAAESNWAVTGLYFYDAAVSDLA"
BASE COUNT          397 a          690 c          656 g          374 t
ORIGIN      
        1 gtgctggagc ggctgatggg aaggtggctg tcggatgccg gcgattggac tgaagaggcc
       61 gatctcgcaa ccgctttgaa ccaagccgcc gcccgcatcc tcgcggtcgc ccttacgccg
      121 gaggccatcg ccctgcaccg cctgctgatc gcggaaagcg cccgcttccc ggaactgccg
      181 ctgatgctgg gccaggcggg ggcaggcgaa ggaacttcac gcatccaggc aatcctgtcc
      241 cgagcagtcg ctgcgggaag gctgccgcca ggggacatgg cgtttgcggc cgaacaattc
      301 ctccatctct tgctggcggg gccgcagcgt cgggcgctcg gcctgggctc gaaacttgac
      361 gaacaccaga cactggtatg gcgagaaacg tcggtggcac tgtttctatc gggtattgaa
      421 aggtgatggt tgccgcgcgg cctaaaataa tgttaccgca cgcgccatag aacggcaggg
      481 atacgtgatt tcatgaagct tttgctgacc ggcgggtgcg gatttatcgg ttcggccgtg
      541 gtccggcacc tgctacgtca aaccgaccat gccgtggtca acgtcgataa aatgacctat
      601 gcggcgtcgg aagacgcgct ggaagactgt caccggcaca accgccacat cctggtgaag
      661 gccgatatcg ccgaccccgc ttccatgcgc caggtcttcg aagcccacga tccggacgcc
      721 gtgatgcacc tggcggcgga aagccacgtt gaccggtcga tcgacggccc cgccgacttc
      781 gtgcagacca atgtcgtcgg caccttcatc ctgctggaag cgtcgcgccg gcattacaac
      841 aacttgtcct ctgaccggaa ggcgatgttc cggtttcatc atatttccac cgacgaagtt
      901 ttcggcgcgt tggaatatgg cgatccgcca ttcaccgaaa ccacgccata cgatccgcgc
      961 agtccctact ccgcgaccaa agccgcatcc gaccacctgg tccgcgcttg gagccatacc
     1021 tacaagcttc cgaccatcgt ctcgaacacc tgcaacaact acggtccctg gcaattcccc
     1081 gaaaagctga tcccgctggt taccctgaac gcgctggaag gcaaggagct cccggtctat
     1141 ggcgacgggt ccaacatgcg cgactggctg ttcgtcgaag accacgccga ggccttggtc
     1201 cgcatcgtcg aacaggggat cccgggtgag acctacgcga tcggcggccg gcagccgcgt
     1261 agcaacctgc aggtggtcaa ggcgatctgc gcccatctcg acaagcgcat tcccgtcccg
     1321 aacggcccgc gcgagcggct gatccgttac gtgacagacc gccccggcca cgatttccgc
     1381 tatgaaatcg atccctcgcg cagcgaacag gcgctgcgat ggaaagcgcc gcacgacttc
     1441 gagacagggc tggcccgcac catcgactgg tatctggaca accggacctg gtgggaagcg
     1501 ttacgagcgg gccggtatgc cggacagcgg ctgggtactt cgaccgacag caaggaaaaa
     1561 gccgcatgaa gggcatcctg ctcgctggcg gatccggcac gcgcctgcac ccgatgaccc
     1621 tggcgtcgtc aaagcaattg ctgcccgtct atgacaagcc gatggtctac tacccgctct
     1681 cgacgctgat gctcgcgggg atcagggaca tcctgctgat ctccacgcct gaggacctgc
     1741 cgcagttcaa acggctgctc ggtaacggct cgcgcttcgg tgtccggttc cagtacgccg
     1801 aacagccgaa gccggacggg atcgcccagg ccttcctgat cggccgcgac tggctggccg
     1861 gcgaggcctg cgctctcgct ttgggcgaca acctgatcca cgccgaccat ctttcgacgt
     1921 tgctgcggac ggcggccgcg aggccagtgg gagcgaccgt gttcgcctac caggtccgcg
     1981 atccggagcg ctacggcgtg gtgtcgttcg atgcggccgg gcgcgtggtc gatatcgtcg
     2041 agaaaccgac cgcggcggag tccaactggg ctgtgacggg cctttacttc tacgacgcgg
     2101 cggtcagcga tctcgct
//
LOCUS       DASWQN010000633         4233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2303727, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000633 DASWQN010000000
VERSION     DASWQN010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303727"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VGC82_10740"
     CDS             <1..423
                     /locus_tag="VGC82_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0423789.1"
                     /translation="VFFQMKSRDTFAPIGPYLVTKDEIADPQKLSINLWVNGELKQSF
                     NTDDMAHNIARCIEWVTSIHTLEPGDILATGTNHRGLSSFMDGDVVELETQGLGRLRF
                     NVRDELKRTWSRETRLDRQQKNQEGPTHQLTGKYAKPG"
     gene            643..1575
                     /locus_tag="VGC82_10745"
     CDS             643..1575
                     /locus_tag="VGC82_10745"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY0423790.1"
                     /translation="MKIVACNSNRPLAEAVAEILKLPLTNASIRRFADMEVFVEIREN
                     VRGEDVFVIQSTSFPANDNLMELLVTLDALRRSSARRITAVIPYFGYARQDRKSAPRT
                     PISAKLVANLITEAGANRVLTMDLHAGQIQGFFDIPVDNLIAEPLFSRDIQERYQGRD
                     IMIVSPDVGGVVRARAIATRLNCDLAIIDKRRERAGHSEVMNIIGDVVGRDCILIDDL
                     VDSGGTHCNAAEALVKRGARSASAYVTHGVLSGAAVARIAASPIEMMTITDSIQPTDE
                     VLRCSNVRELTIAPLLAEAMRRISDESSVSSLFD"
     gene            1772..2710
                     /locus_tag="VGC82_10750"
     CDS             1772..2710
                     /locus_tag="VGC82_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HEY0423791.1"
                     /translation="MDHIIGQAPTASANATANMIVDGHQKTFMQDVIEASRSVPVLVD
                     FWATWCGPCKQLTPTLERVTKAAGGRVKLVKIDIDQNQALVRQLAQIGLPMQSVPTVA
                     AFWQGQIADVFQGAQPESEVKRFVEALLKASGGSMPAADLLAEAKAALEQGDPQQAGE
                     LFSALIGEEPENGDAWGGLIRALIAMDQEEAAEEALSQVPEKLAEHAEVNGARSALVL
                     AREGRAAKDQLQTFQARLAADPADHQARYDLATALNAMNQREQAAEALLEIIRRARTW
                     NEGAARLQLLKFFESWGSDDPATMAARRRLSALLFS"
     gene            2712..3377
                     /locus_tag="VGC82_10755"
     CDS             2712..3377
                     /locus_tag="VGC82_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY0423792.1"
                     /translation="MAMFHPRLEDLPPEFAVFPLTGALLLPRGKLPLNIFEPRYLAMV
                     QDSLATGRMFGMIQPDPHAAAAAQGDGLYRVGCLGRLSSFSETDDGRLLIALTGLIRF
                     TIAAELEPRHGYRRVRGDFSKYVDDLTLDRPQVEIEREKILSALRAYFGQRKVDANWE
                     AIRGLSDDTLVITLAMACPFEAVEKQALLEAPTDTDRAATLLALLQMGAAGPDLPPGH
                     SVS"
     gene            3386..3616
                     /locus_tag="VGC82_10760"
     CDS             3386..3616
                     /locus_tag="VGC82_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY0423793.1"
                     /translation="MPDPSPSADPVTPSPAPAESAVDPRLLEILVCPLTKAPLEYDRT
                     ANELISRKAGLAYPIRDGIPIMLPEEARKLEE"
     gene            3616..3972
                     /locus_tag="VGC82_10765"
     CDS             3616..3972
                     /locus_tag="VGC82_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEY0423794.1"
                     /translation="MPVPTLIRLDRAARVLHVTFDDGTDYALPAEYLRVESPSAEVQG
                     HNPAQKQTVAGRRHVGIIGVEPVGQYAVRLVFDDLHDTGIFSWDYLHELGREHDHRWA
                     GYLDALASRGLSRDPR"
BASE COUNT          815 a         1331 c         1306 g          781 t
ORIGIN      
        1 gtgttcttcc agatgaagtc gcgcgacacg ttcgccccga tcggacctta cctggtgacc
       61 aaggacgaaa tcgccgatcc gcagaagctg tcgatcaacc tgtgggtgaa cggcgaactg
      121 aagcagtcct tcaacaccga cgacatggcg cacaacattg cccggtgcat cgagtgggtg
      181 acatccatcc atacactcga accgggcgat atcctggcga ccggcaccaa ccatcgcggc
      241 ctgtccagct tcatggacgg cgatgtggta gagctggaaa cgcaaggtct cggacggctt
      301 cggttcaacg tccgcgacga actgaagcgc acctggtcgc gtgaaacccg gctggaccgg
      361 cagcaaaaga accaggaggg cccgacgcat cagcttaccg gaaaatacgc caagccaggc
      421 tgaccacagg accggcatcc gtcgcgaggc agcgtgcctc gcgcggcggc cacactgcct
      481 gtggcaggtt agccacactg cctgtgtcag gttagccaca ttgtctgtgt caggttagtc
      541 agggtctgtg tcaggttaat ggacatgcga cagggcggtt gctagaagcc cgttgtttcc
      601 ggcaccccga ccggttcggc gttcgagcca gcggccctgc tcatgaagat cgtagcctgc
      661 aacagcaatc gcccgttggc cgaggctgtc gctgaaatcc tgaaactgcc gctgacgaat
      721 gcctccatca ggcgcttcgc cgatatggaa gtgttcgtcg aaatccggga gaacgtacgc
      781 ggcgaggacg tcttcgtcat ccagtcaacg tcgtttccgg cgaacgacaa ccttatggag
      841 ctgctggtca cgctggacgc cctgcggcgt tcgtccgcgc ggcgcatcac cgctgtcatt
      901 ccgtatttcg gctatgccag gcaggatcgg aagtcggcgc cgcgcacgcc aatctcggcg
      961 aaactggtgg cgaacctgat caccgaggcg ggcgccaatc gcgtcctgac gatggatctc
     1021 catgccggcc aaatccaggg attcttcgac atcccggtgg ataacctgat cgccgaaccg
     1081 ttgttcagcc gcgacatcca ggagcgttac caaggccgcg acatcatgat cgtctctccc
     1141 gacgtgggtg gcgtcgtgcg cgcccgggcg atcgcaaccc ggctgaactg cgaccttgcc
     1201 atcatcgaca aacgccgcga acgagccggc cattccgagg tgatgaacat catcggcgac
     1261 gtcgtaggcc gcgactgcat tctgatcgac gaccttgtcg actccggcgg cacccattgc
     1321 aacgcggcgg aggcgctggt gaagcgcggc gcccgttcag ccagtgccta cgtcacccat
     1381 ggcgtgctgt ctggcgccgc cgtcgcccga atcgcggcat cacccatcga gatgatgacg
     1441 atcaccgatt cgattcagcc gacggatgaa gtactgcgct gcagcaacgt tcgcgagctg
     1501 accatcgccc cgttgctggc cgaggcgatg cgccgcatca gcgatgaaag ctccgtcagt
     1561 tctttgtttg actgacaagg gaaccatccg aacgttccgg catgatgcct tatgcgattg
     1621 cttcgactgg atgaaacgct tccactggac gccgccgccc cgaaacggca tggtccgcac
     1681 ctggacgacc tacctcaaac ggacgcttgt ttgtttgcgg gtcgcctaca tctagactcg
     1741 aacgaccaaa gccgaccggg aagcacgcgt aatggaccac atcatcggcc aggcaccgac
     1801 tgcctctgcc aacgccaccg ccaacatgat cgtcgatggc catcagaaga ccttcatgca
     1861 ggatgtgatc gaagcttcac gctcggttcc cgttctggtg gatttctggg caacctggtg
     1921 cggcccctgc aagcaactca cgccgacgct tgagcgggtc accaaggccg cgggcggacg
     1981 cgtcaagctg gtgaagatcg acatcgacca gaatcaggcc ctggtccgcc agttggccca
     2041 gattggcctt cccatgcagt ccgtgccgac tgttgcggcc ttctggcaag gccagatcgc
     2101 ggacgtgttc cagggcgccc agcccgaatc cgaagtcaag cgtttcgtcg aagccttgtt
     2161 gaaggcgagc ggcggttcga tgcccgcggc agacctgctc gcggaggcaa aggcggcgct
     2221 ggaacagggc gatccgcagc aggcgggcga gctgttcagc gcactgatcg gcgaggagcc
     2281 ggaaaacggc gacgcctggg gtggcctgat ccgcgccttg atcgcgatgg accaagagga
     2341 agccgccgaa gaggcgctgt cccaggtgcc cgaaaaactt gccgaacacg ccgaggtcaa
     2401 cggcgcccga agtgcgcttg tgctggcaag ggaagggcgc gctgccaagg atcaactgca
     2461 gacttttcag gcgaggctgg ctgccgaccc cgccgaccat caggcgcgct atgacctcgc
     2521 gacggcgctg aatgcgatga accagcgcga gcaggccgcc gaagccttgc tcgagatcat
     2581 ccgccgcgcc cgtacctgga atgaaggcgc tgccaggctg caactgttga agttcttcga
     2641 gtcctggggc tccgacgatc ctgcgaccat ggccgccagg cgacgtctat cggcgctgct
     2701 gttcagctaa catggcgatg tttcacccgc ggcttgagga tctgccgccc gaattcgcgg
     2761 tttttccgct gaccggagcg ctgctgttgc cgcgaggcaa attgccgctg aacattttcg
     2821 agccgcgtta tctggcgatg gttcaggatt cgctggccac cggccgcatg tttggcatga
     2881 ttcagcccga tccccacgcg gcggcagccg cccaaggcga tggcctctac cgtgtcggat
     2941 gcctgggcag actttcgtcg ttcagcgaaa ccgacgacgg ccggctgctc atcgcactga
     3001 ccggattgat ccgcttcacg attgctgccg aactggaacc gcggcacgga taccggcggg
     3061 tgcgcggcga tttcagcaag tacgtggacg acctgacgct ggataggccg caggtcgaga
     3121 tcgagcgcga gaaaatattg tcggccctgc gcgcctattt tggtcaacgc aaagttgacg
     3181 ccaactggga ggccattcgt ggcctttctg atgacacgct ggtcatcacg ctcgccatgg
     3241 cctgtccatt tgaagcagtg gagaagcaag ctttgctgga ggctccgaca gataccgacc
     3301 gagcggcaac gctgttggcg ttgttgcaga tgggtgcggc cggtccggat ctgccgccgg
     3361 gccattcggt cagctagaat aacccatgcc agatccctca ccttcggcag accccgttac
     3421 cccgtccccc gccccggccg agtcggcggt cgatcctcgg ctgctggaga ttttggtctg
     3481 cccgttgacc aaggctccgc tcgaatacga ccgcaccgcc aacgaactga tcagccgtaa
     3541 agccggtttg gcgtatccaa ttcgcgacgg gatcccgatc atgctgccgg aagaagcccg
     3601 caagctggag gaatgatgcc ggtccccacg ctcatccgcc tggatcgtgc ggctcgggta
     3661 ttgcatgtca cgttcgacga tgggaccgat tacgcactgc ctgccgaata tttgcgtgtg
     3721 gaaagtccaa gcgcggaggt tcaaggacac aatcccgcgc aaaagcagac ggtcgccggg
     3781 cggcgccacg tcggcatcat cggggtcgag ccggtcgggc aatatgcggt ccggcttgtt
     3841 ttcgacgacc tgcatgatac cggcatcttc tcctgggact atctgcatga gcttggccgc
     3901 gagcatgacc atcgctgggc cggatacctg gacgcactcg catcgcgggg actatcgcgc
     3961 gatccgcgtt agctgcgtgt cggtcgcggg atgcaatgac gaaaatggtt tggcatcgct
     4021 tggaccattg gcctgtggaa cattggcccg tggaccattg gcctatggac gattggcccg
     4081 tgccacgttg gccggccacg cttttcatga cgccgaggga aatagcttat gccgccccgc
     4141 cctcgtcgtt tgagcgaagc cgaccaggca ctttgggcag cgtacgccag ccaactcgcg
     4201 cctttgcggg gccgagtcag ccggaagccg ctg
//
LOCUS       DASWQN010000634         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_588688, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000634 DASWQN010000000
VERSION     DASWQN010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588688"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VGC82_10770"
     CDS             complement(<1..240)
                     /locus_tag="VGC82_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423795.1"
                     /translation="MAASINYLSCGQESPSAILLPAFICHSVAAESNFRFCCYRSPTQ
                     KNFLIQMSSPTPPVATHAAPIETLAGLVERVTYHNN"
     gene            245..916
                     /locus_tag="VGC82_10775"
     CDS             245..916
                     /locus_tag="VGC82_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0423796.1"
                     /translation="MQPFPAGFEIKDIATNGTTLSVRVGGQGPAVVLLHGYGETGDMW
                     APLVTELARDHTVVAPDLRGMGLSARSAGGYDKKTQGQDIAGLLDALGIERADLVTHD
                     IGNMVGYAFAAQYPDRVIRFALMDAPLPGIGPWEEILKSPLLWHFRFGGPDMERLVAG
                     RERIYLDRFWNEFRLIRSLLARLRANIMPNYTLSLARCMPVSASSPLSTRMPLTTRPL
                     SPGAS"
     gene            919..1098
                     /locus_tag="VGC82_10780"
     CDS             919..1098
                     /locus_tag="VGC82_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423797.1"
                     /translation="MPVMAVGGEKSFGPTMAVVMRAAATDVQEQVILASGHWLMEEQP
                     MATVAAVRAFLNRQH"
     gene            complement(1343..2299)
                     /locus_tag="VGC82_10785"
     CDS             complement(1343..2299)
                     /locus_tag="VGC82_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HEY0423798.1"
                     /translation="MGEVHDILEAKGREGALQAGLERAIVDAASAYMGDEDNALGFVY
                     AGWAQCALPHSKLLPDAPWGIRAEKLRLLVEPGRRPVGDANETEFVGVPFGAHARLIL
                     LYLQTEALRTGNREVELGGSLREWLSRIGVSYGGATAKSVRNQAERISMCKLSFHFTE
                     GKKTGLLQQTIVDRALFLEPEERHSHPRQRRLNLEAAKLSEGFFEQLRRHPVPIEEAA
                     VKVLNNKSAGLDCYLWLAYRLHVLTGNVPVSWPALKGQFGTAFTKLAGFKSWFLAPDG
                     PLALALSVYPDAKVAVEENGVVLKPSKPPVPPRLMASYTPRS"
BASE COUNT          517 a          733 c          731 g          533 t
ORIGIN      
        1 gttgttgtgg taggtcactc gctcaaccaa gccagcgagc gtctcgatcg gcgctgcatg
       61 ggtggctact ggcggtgtgg gagacgacat ttggatcaga aaattctttt gagtggggct
      121 ccggtagcaa cagaaccgaa agttcgattc ggccgcaacg ctgtggcata taaaggcagg
      181 cagcagaatt gccgacggcg attcctggcc gcaagaaagg tagttgatgc tcgccgccac
      241 agatatgcag ccgttcccag ccggcttcga aattaaggac atagctacga atggcacgac
      301 cctgtctgta agggtcggcg gacagggacc tgctgtcgtc ctgcttcacg ggtacggaga
      361 aacaggcgat atgtgggcgc cgctcgtaac agaacttgcc cgcgaccata ccgttgttgc
      421 ccccgatctt cgcgggatgg ggctctccgc ccgctctgcc ggaggctacg acaagaagac
      481 acagggtcag gatatcgccg gcctgctcga cgcactcggg atcgagcggg cggacctcgt
      541 aacgcacgac atcggcaaca tggtgggata cgccttcgct gcgcagtatc ctgaccgggt
      601 catccgcttc gccctcatgg acgcgccgct gccggggatc gggccgtggg aggaaatact
      661 caagagcccg ctattgtggc atttccgctt tggcggccct gatatggagc gcttggttgc
      721 aggccgcgaa cgcatctacc ttgatcgctt ctggaacgag tttcggctga tccgaagtct
      781 tttggcgagg cttcgcgcga acattatgcc aaactatacg ctcagcctgg cgcgatgcat
      841 gccggtttca gccagttcgc cgctttcgac caggatgcca ttgacaacaa ggcctttgtc
      901 gcccggggca agctgacaat gccggtgatg gcggtgggtg gcgaaaagtc atttgggcca
      961 accatggcgg ttgtcatgcg tgccgcagcg acggatgtgc aggaacaggt cattttggct
     1021 tccggtcatt ggctgatgga ggaacagcca atggccacgg ttgcagccgt gcgtgccttc
     1081 ctgaatcggc aacattgatc gccgcacccg ccaccactgt tggagttccg gtagcaacgg
     1141 aaccaagagt gcgattggag acaccgccat gacatggcac gctccgctgc gagccggcta
     1201 tgcatcaact cgtgcatcta tctccggcca gcaaaccaac ccgcggcttt gggttgaatc
     1261 gaactttcgg ttccgttgct accggaccct cgatgtaggg gttttatcgc cttcaggctg
     1321 ccccaagtgg ggactctgct gatcacgacc gaggcgtgta actagccatt aaacgaggcg
     1381 gtactggcgg ctttgaaggc ttgaggacga cgccattctc ctcgactgcc accttggcgt
     1441 ccggatagac agacaaagca agcgccaacg gcccatcggg ggctaagaac caagatttaa
     1501 agcctgcaag tttggtgaac gctgtcccga actgcccctt taatgcaggc caggatacgg
     1561 gcacgttgcc ggttaggacg tgcaggcgat acgcgagcca aagatagcaa tcaagcccag
     1621 cggacttgtt gttgagcacc tttacggccg cctcctcaat aggcactgga tgccgccgta
     1681 gctgctcaaa aaatccttcg gagagctttg ccgcctccag attcagccgc cgctggcggg
     1741 ggtgtgagtg cctctcctcg ggttcaagaa agagcgcgcg gtcaacgatt gtctgctgga
     1801 gcaacccggt cttcttgcct tctgtgaaat ggaaactcag tttgcacatc gagatccgtt
     1861 cagcctggtt tcgcacggat ttggctgttg ccccgccata actgacgccg atccgcgaca
     1921 gccattcgcg caacgagccc cctagctcca cctctcggtt ccccgttctg agcgcctcgg
     1981 tctgcaaata cagcaggatc aagcgagcat gagcaccgaa cgggacgccc acgaattcag
     2041 tctcgttggc atctcctacc ggtcgtcggc caggttccac cagtagtcgg agtttctctg
     2101 cccggatgcc ccagggagca tcaggaagca acttggaatg aggtagggcg cactgagccc
     2161 atcccgcgta aacgaaccca agagcgttgt cctcatcgcc catgtaggcg ctggccgcgt
     2221 ccacgattgc tcgttcaagg ccagcttgta gagccccttc gcgtcccttg gcctccagga
     2281 tgtcgtgaac ctcgcccatg ggtctgcctc atcaaccgcc agtcgtttgt ttcaaatccg
     2341 acgctgctag aacgcttacc agggatgccg gcggctgtcg ctggggactc tgctgagcga
     2401 gctattagaa agaatattag atacttatat tagcttgctc agcagagtcc ccaggataac
     2461 tttgcttatg tcattgattt catttaatca cagccctcct taaacaaggc cgtg
//
LOCUS       DASWQN010000635         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_456598, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000635 DASWQN010000000
VERSION     DASWQN010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456598"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1312
                     /locus_tag="VGC82_10790"
     CDS             104..1312
                     /locus_tag="VGC82_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEY0423799.1"
                     /translation="MHKSFLKAGHVPTLFASFLYFDLSFMVWVLLGPLGVAIAKTLNL
                     DPAHKGLMVATPVLAGAALRVVLGLLVDRYRAKRVGIAAQIVVIIGLLAAWTIGIDSF
                     GATLLLGCVLGLAGASFAVALPLASAWYPPEHQGTALGIAGAGNSGTVLAALFAPMLA
                     IAFGWQNVIGLAAIPLFLTLCVFIFAAKDSPNTPAPKPLAAYFALLKVGDCWWFMFFY
                     GVTFGGFVGLASSLTIYFNDAYGLSPVVAGYCTAAVVFVGSLVRPFGGAVADRVGGVR
                     ALVVLYAVAASAFATISTGLSTIYLAMPVFLIGILALGMGNGSVFQLVPQRFKREIGV
                     MTGLVGMSGGVGGFYLASSLGYSKQLTGSYGPGFLVFALLAAVALVGISGVRHRWRTT
                     WTALYGAARV"
     gene            1332..>1930
                     /locus_tag="VGC82_10795"
     CDS             1332..>1930
                     /locus_tag="VGC82_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0423800.1"
                     /translation="MIAPRRSRLVLVGNGMAGVRALEETLSRAPHQFSISVFGAEPHG
                     NYNRIMLSPVLAGEKTFEAITTHPRSWYEENGIELIAGEPVVAIDREARTVTGALGTT
                     RPYDVLLLATGSNPVIIPLPGHKLTGVIGFRDIGDVETMLAASRAGEHAVVIGGGLLG
                     LEAANGLALNGMDTTVLHIMPTLMERQLDPVSAGLLKADL"
BASE COUNT          289 a          622 c          618 g          401 t
ORIGIN      
        1 acctccccaa agacgggctt gtgccggata ctaggcgcta agcgcttcac gcccatcgac
       61 cggcacgcca acgacggctt gcccccgaga gtgatccact caaatgcaca agagtttcct
      121 caaggccggt catgtaccga ccctgttcgc gtcgttcctg tatttcgacc tcagcttcat
      181 ggtctgggtg ttgctcggcc cgctgggcgt ggcgatcgcc aagacattga atctcgaccc
      241 ggcgcataaa ggcctgatgg ttgccacgcc ggttctcgcc ggcgccgccc ttcgcgtggt
      301 gctgggtctg ctggtcgatc gctatcgggc gaagcgcgtc gggattgccg cacaaatcgt
      361 cgtcattatc gggctgctcg ccgcctggac gatcggcatt gacagcttcg gcgccacgct
      421 gctgctggga tgcgtgctgg gcctcgccgg ggcatcgttc gccgtggcgc tgccgctggc
      481 atccgcctgg tatccgccgg agcaccaggg caccgcgctc ggcattgccg gcgcgggcaa
      541 ttccggcacc gtcctggctg cgttgttcgc ccccatgctg gcgatcgcgt tcggctggca
      601 gaatgttatc ggcctggcgg ctatcccgct gttcctgact ttgtgcgtgt tcatcttcgc
      661 cgcgaaggac agcccgaaca cccctgcgcc gaagcctttg gcggcctact tcgccctgct
      721 caaggtcggc gactgctggt ggttcatgtt tttctacggc gtgacgttcg gcggcttcgt
      781 cggcctggct tcatcgctga ccatctactt caacgacgcc tacggcttgt ctccggtcgt
      841 agccgggtac tgcaccgccg cggtcgtctt tgttggatcg ttggttcgtc cgttcggcgg
      901 cgctgtcgcc gaccgcgtcg gcggagtccg ggccctggtg gttctgtatg ccgtcgccgc
      961 cagcgcgttc gcgacgatct ccaccgggct gtccacgatc tatctggcga tgccggtgtt
     1021 cctgatcggc atactggcac tcggaatggg caacggttcg gtatttcaac tcgtgccgca
     1081 gcgcttcaag cgggaaatcg gcgtcatgac tgggcttgtc ggcatgagcg gcggcgtcgg
     1141 cgggttctac ctggcctcca gccttggtta ctccaagcaa ctgaccggca gctacggtcc
     1201 tggtttcctg gttttcgcct tgctggcggc ggtcgccctg gtcggcatca gcggcgtgcg
     1261 ccaccgctgg cggacgacct ggaccgcgct ttatggcgcc gctcgcgttt aacggagaga
     1321 tcgacatgaa catgatcgct cctcgccgtt cccggctggt actggtcggg aacgggatgg
     1381 caggcgtgcg tgcgctggaa gaaaccctga gccgtgctcc gcatcaattc tccatctccg
     1441 tgttcggtgc ggaaccgcac ggcaactata accgcatcat gctgtcgccc gtgctggcgg
     1501 gcgagaagac cttcgaagcg atcacaaccc acccaaggtc gtggtacgag gaaaacggca
     1561 tcgaactgat cgccggcgag cctgttgtcg cgatcgaccg cgaggcaagg acggtgaccg
     1621 gcgcattggg caccactcgt ccctacgatg tgctgctgct ggccaccggg tcgaacccgg
     1681 tgatcattcc gctgccggga cacaagctga ccggggtgat cggcttccgc gacatcggcg
     1741 atgtggaaac gatgctggcg gccagccgag ccggagaaca tgcggtcgtc atcggcggcg
     1801 gcctgctcgg tctggaggcg gcgaacgggc tggcgctaaa cggcatggac accaccgtgc
     1861 tgcacatcat gccaaccttg atggagcggc aactcgatcc agtctcggcc gggttactga
     1921 aagccgatct
//
LOCUS       DASWQN010000636         7096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1248567, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000636 DASWQN010000000
VERSION     DASWQN010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248567"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /gene="rho"
                     /locus_tag="VGC82_10800"
     CDS             complement(<1..1182)
                     /gene="rho"
                     /locus_tag="VGC82_10800"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY0423801.1"
                     /translation="MHLAELKKKTPAELVNFAESVQVENASSLRKQDIMFAILKNLAD
                     SDQAIQGDGTLEILSDGFGFLRSPEANYLPGPDDIYVSPSQVRRFGLRTGDTVDGQIR
                     APRDGERYFALLKVDTVNFEPPDAVRHRVNFDNLTPLYPEERLKMEMEKFESVAKGPT
                     KDFTPRVIDLISPVGKGQRALIVAPPRTGKTVMLQNIAAAIAANHPEVFLIVLLIDER
                     PEEVTDMARSVNGEVISSTFDEPATRHVQVTEMVLEKAKRLVEHKRDVVILLDSIMRL
                     ARAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMD
                     EVIFEEFKGTGNSEIILDRKLSDKRSFPAIDITKSGTRKEELLVERSVLSKMWVLRRI
                     LN"
     gene            complement(1445..4159)
                     /locus_tag="VGC82_10805"
     CDS             complement(1445..4159)
                     /locus_tag="VGC82_10805"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY0423802.1"
                     /translation="MTLDSRFAAGEIEPRLYDGWERAGSFACDPDSNAVPFTVMIPPP
                     NVTGSLHMGHALTFTIQDTLVRWRRMQGRDALWQPGTDHAGIATQMVVERLLAAEGKD
                     RREMGREAFVERVWQWKAESGGTITTQLRRLGASLDWPRERFTMDDGLSAAVKETFVK
                     LHDQGLIYRDRRLVNWDPKLQTAISDLEVENKEVKGSLWYIRYPIEDEPGQFIPGRFI
                     IVATTRPETMLGDTAIAVHPEHPIYGALVGKRAILPLVGRSIPIVADLYADPEKGTGA
                     VKITPAHDFNDFEVGRRHNLPMPSVLDRQALVTLAEIEELLTEIPGIADPVFIRTLAG
                     QARFAARSAIVAELERLELLDRIEPHTHQVPFGDRGGVPVEPRLTTQWYCNAEVLAKP
                     AIAAVETGKTVFYPRQWENTFFAWMRDIQPWCISRQLWWGHRIPAWYGPDGTVFVGRD
                     EAEAAARARDHYGHDEILVQDEDVLDTWFSSALWPFSTLGWPEKTKEVQRYYPGDVLV
                     TGFDIIFFWVARMMMMGLHMMGDVPFRTIYIHGLVRDERGQKMSKSRGNVIDPLELID
                     SYGADALRFTICALTGPGRDVKLGAARVQDYRGFVTKLWNAARFCEMNGMQPQAGFDP
                     ADASLPLSRWLLAETNAAIRDATDALEAYRFDEYAASCYRFVWNTFCDWFLEFSKPLL
                     AEGADPSDAAEIRSVGAHVLGQILRLLHPVIPFVTEELWDRFGYGAPGSLIETTWPEP
                     VSVPGAEQARVELDWVVRLIGLVRSVRAEMNVPPSKPSPVLLQGASAEALVRVERWIE
                     AIRRLARASEVSALVGDVPKGSAMAVLEETTIVLPLEGLIDIGMERARLAKDRDKLVA
                     DANKTAQKLANADFVSRAPEEVVTENRDRLESARSEIARLQAALDRLE"
     gene            complement(4244..4807)
                     /locus_tag="VGC82_10810"
     CDS             complement(4244..4807)
                     /locus_tag="VGC82_10810"
                     /inference="COORDINATES: protein motif:HMM:NF022155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HEY0423803.1"
                     /translation="MSDKPSNPAPTPSAADPSMEEILASIRRILNEDEVPAVPQAGTE
                     APAGDDVLILEAAMLVPSDKPSPGVVEAELEPEPIAPEAKAPRQAMEAVTSPPQADPF
                     EQDAPADDLLAPETAAAAAFSIGSLVRTLTAGRTTQVYAGGPTLEDIVRGELRPLLKQ
                     WLDANLPPVVERLVRAEIEKVVGRAVS"
     gene            complement(4822..6357)
                     /locus_tag="VGC82_10815"
     CDS             complement(4822..6357)
                     /locus_tag="VGC82_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY0423804.1"
                     /translation="MGLRHGLLASFSLVALTAAAGGQTPARSPLSQPPAMAPAVRSAE
                     GAAPKGKAAPGTAVAAGAAAAQYIPHTLTEALAATYTYQPALQAERARLRATDENVPT
                     ALAGWRPTMVLGGQAGYGDGVSRAYTAQLGRVIAAQTDRVIGTAQATVTQNVYTGGKT
                     QAGVNRSKNQVMAERATLLAQEQTSFNNAVAAYVGVIQTQQLLALQINNEQVLSKQLQ
                     ATNDRFRVGEITRTDVAQAEAALQGARAQRETAEGNLQTQRGNFQQFVGFAAPSDLIE
                     PQPLNLPVNSEQEASALAAANNPNVVTALFNDAAAKDAVDLALSALLPTVSLNGQTFQ
                     QTNAGGRSTSNNGFQVTASLNVPIYQGGSEYAAVRQARQQQQQTNRLIDDARRTAVQN
                     AVQAWGTLVAAKASADSTRSQIRANQIALEGTEREAIVGSRTTLDVLNATQALLSSRT
                     TLVQNLAQVITASYAVAVAIGRLTARDLHLPVPLYDETAYYQAVKDRWVGLGDYATSQ
                     PGR"
     gene            complement(6593..>7096)
                     /locus_tag="VGC82_10820"
     CDS             complement(6593..>7096)
                     /locus_tag="VGC82_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY0423805.1"
                     /translation="DVPLGNGRFMMEPMVFARLLQAARLQENERVLVVGAATGYGAAV
                     LADCGCRVTALEEDPALLAMANAVLPEQAPGVTVVSGPLAAGWASGAPYDLILIEGAV
                     PEIPPALAAQMHQETGRIITAIHAPGRVTQATIGEMTSFGLGLSSIFDCATPPIPSLR
                     KAPAFSF"
BASE COUNT         1219 a         2275 c         2236 g         1366 t
ORIGIN      
        1 attgaggatg cgccgcagca cccacatttt cgacagcaca ctgcgttcga ccaggagttc
       61 ttccttgcgc gtgcccgact tggtgatgtc gatggccggg aacgaccgct tgtccgacag
      121 tttgcgatcc agaatgatct cggaattgcc ggtgcccttg aactcttcaa agatcacttc
      181 gtccatgcgg ctgccggtgt cgatcagcgc cgtggcgatg atggtcaaag agccaccttc
      241 ctcgatgttg cgtgctgcgc cgaagaaacg cttcggtcgc tgcagcgcgt tggcatcgac
      301 accaccggtc aacaccttgc cggatgacgg caccacggtg ttgtaggctc gggccaggcg
      361 catgatgcta tccagcagga tgacaacgtc gcgcttgtgc tcaaccaggc gtttggcttt
      421 ttccagcacc atttcggtca cctgcacatg gcgggtcgcc ggttcgtcga aggtggagct
      481 gataacctcg ccgttgacgc tgcgagccat gtcggtgact tcttccggcc gctcatcgat
      541 cagcagcacg atcaggaata cttccggatg attggcggcg atggcagcgg cgatgttctg
      601 cagcatcacc gtcttgccgg tgcgtggcgg cgccacgatc aacgcgcgct gtcccttgcc
      661 caccggcgaa atcagatcga tgacccgcgg cgtaaagtct tttgtcggcc ccttggcgac
      721 ggactcgaat ttctccatct ccattttcag gcgttcttct ggatacagcg gcgtcaggtt
      781 atcgaagttg acgcgatggc gcaccgcatc gggcggctcg aaattcacgg tatcgacttt
      841 aagcagggcg aagtaacgct ccccatcccg aggcgcgcgt atttgcccat cgacggtatc
      901 gcccgtgcgc aggccaaagc gccggacctg gctcggactg acgtagatat catcggggcc
      961 gggtaagtaa ttggcttccg gactgcgcag gaagccgaac ccgtcggaca ggatttccag
     1021 cgtgccgtca ccctggatgg cttggtcgct gtcggcgagg ttcttaagga tcgcgaacat
     1081 gatgtcctgc ttacgcagcg acgacgcgtt ctcgacttgt acggattcgg cgaaattcac
     1141 cagttcggct ggtgtttttt tcttcagttc agcgagatgc atgaggatgc ctggacgtga
     1201 gggtgcgggt cggacgccgg atcggcctga gcgtgtttag ggcgaacacg ctgcgcggtt
     1261 cgcttgccaa tcggaggttc ttggctctca gaatgtatgg ctcaaggcgt tatgtgcatg
     1321 agcacagtcg cccgacgttc gggagggagt tctatataag gaaaggtctg accggtcgtc
     1381 aatgggttcg accgattctt tcacaattga tgccgcgatg acgcacaagg gcgaacccga
     1441 ccgactactc cagccggtcc agcgccgctt gtaatcgagc aatctcggat cgggcggatt
     1501 caagccggtc gcggttctcg gttaccactt cctccggtgc ccggctgacg aaatcggcgt
     1561 tcgccagttt ctgcgcggtc ttgttggcgt cggcgacaag cttgtcgcga tccttcgcca
     1621 ggcgcgcccg ttccatgcca atatcgatca gcccttccaa cggcagcacg atggtggttt
     1681 cctccagcac cgccatggcc gaccccttgg ggacatcgcc gaccaaggcc gaaacctccg
     1741 atgcgcgcgc caggcggcga atggcctcta tccatcgctc cacccgaacc agcgcttcgg
     1801 cggaggcacc ttgcaacagc accggcgagg gcttcgacgg cggcacgttc atctcggccc
     1861 ggaccgaacg gaccagtccg atcagccgca ccacccagtc cagttcgact cgggcctgct
     1921 ccgccccagg cacgctgacg ggctccggcc acgttgtctc gatcaggctg cccggcgcgc
     1981 catagccgaa acggtcccac agctcttcgg taacgaacgg gatcaccggg tgcaacaggc
     2041 gcaggatctg tcccagcaca tgcgcgccga cagagcgtat ttcagcggca tcggagggat
     2101 cggcaccttc agccagcagc ggcttggaga actccaggaa ccagtcgcaa aacgtattcc
     2161 agacgaaccg gtagcaggaa gcggcgtact catcgaagcg gtacgcttcc agcgcatccg
     2221 tcgcgtcccg gattgcggcg ttcgtctcgg ccagcagcca gcgcgacaag ggcagggaag
     2281 cgtctgcagg atcgaagccg gcttgcggtt gcatcccgtt catttcgcag aagcgtgcgg
     2341 cgttccacag cttggtcacg aagccgcggt agtcctgcac gcgggccgcc ccaagcttca
     2401 cgtcgcggcc cgggccggtc agggcgcaaa tggtaaagcg cagcgcatcg gcaccataac
     2461 tgtcgatcaa ctccagcgga tcgatgacgt tgccgcggga cttcgacatc ttctggccgc
     2521 gctcatcgcg caccagcccg tgaatgtaga tggttcggaa cggcacgtcg cccatcatgt
     2581 gcagccccat catcatcatc cgggcaaccc agaagaagat gatatcgaag ccggtgacca
     2641 gcacgtcacc cggatagtag cgttggacct ccttggtctt ttccggccaa ccaagcgttg
     2701 aaaacggcca cagcgccgaa ctgaaccaag tgtcgagaac gtcctcgtcc tgcaccagga
     2761 tttcgtcgtg tccgtaatga tcgcgcgccc gggcggcagc ctcggcttcg tcccggccga
     2821 cgaacaccgt gccatccgga ccgtaccagg ccgggatgcg atgcccccac caaagctggc
     2881 gcgaaatgca ccagggctgg atgtcccgca tccaggcaaa gaaggtgttc tcccactgcc
     2941 tgggatagaa aaccgtcttt ccggtttcca ctgccgcaat cgccggcttg gccagaacct
     3001 cggcattgca gtaccactgt gtagtcagcc tcggctccac gggcacgcca ccgcgatcgc
     3061 cgaacggcac ctgatgtgtg tgcggctcga tcctgtcgag cagttccagc cgttccagtt
     3121 ccgccacgat ggccgaacgg gcggcaaacc gagcctgtcc ggcgagggtg cggatgaaga
     3181 ccggatcggc gatgccgggt atttcggtca gcagttcctc gatctcggcc agtgtgacca
     3241 gcgcctgacg gtccagtacc gagggcatcg gcaggttgtg ccggcggccc acctcgaagt
     3301 cgttgaagtc atgcgccggg gtaatcttaa ccgcgccggt gcctttttcc ggatccgcgt
     3361 agaggtcggc gacgataggg atcgaacggc cgacaagcgg caggatggcg cgcttgccga
     3421 ccagcgcgcc gtagatggga tgttccggat gcaccgcgat ggcggtgtcg cccagcatcg
     3481 tttccggccg cgtcgtggcg acgattataa accgccctgg gataaactgg ccgggttcgt
     3541 cctcgatcgg gtagcggatg taccagagcg agcctttgac ctccttgttc tcgacctcga
     3601 ggtccgagat cgctgtttgc agctttgggt cccagttcac cagccgccgg tcgcgataga
     3661 tcaggccctg atcgtggagt tttacaaagg tttccttcac cgcggcggac aagccgtcgt
     3721 ccatggtgaa gcgctcgcgc ggccagtcca gcgaggctcc caggcggcgg agctgcgtgg
     3781 tgatcgtgcc gcccgactcc gccttccact gccagacgcg ctccacgaac gcttcgcggc
     3841 ccatctcgcg gcgatctttt ccctcggccg ccagcaaccg ctcgacgacc atctgcgtgg
     3901 cgatgccggc atggtcggtg ccgggttgcc aaagcgcgtc gcggccttgc atgcggcgcc
     3961 agcggaccag cgtgtcctga atggtgaagg tcagcgcgtg acccatatgc aggctgccag
     4021 tgacgttcgg cggcgggatc atgacggtga agggcaccgc gttcgagtcg gggtcgcacg
     4081 cgaagctgcc tgcgcgttcc cagccatcgt acagccgggg ctcgatctcc ccggcggcaa
     4141 acctgctatc cagtgtcatc gcgctctccc gcggtccggc gcatctgtcc ggggtccgcg
     4201 tcaccgattg tccgaaccac catcgcggcg ggcgcgcttg ttatcaggaa acggcgcggc
     4261 cgacgacctt ctcaatttcg gcacgaacca ggcgctccac aaccgggggc aggttggcgt
     4321 ccagccattg cttcagcagc ggacgcaatt cgccacgcac gatgtcctcc agcgtcggtc
     4381 cacccgcata cacctgggtc gttcgcccgg cggtcagggt gcggaccagg ctaccgatcg
     4441 agaacgcggc ggcggcagcg gtttccggtg ccagcagatc gtcggccggg gcatcctgtt
     4501 cgaacgggtc ggcttgaggg ggggacgtta cagcctccat tgcctggcgc ggcgccttgg
     4561 cttccggcgc gatcggttcg ggttccaatt ccgcttcgac cacacccgga ctgggtttgt
     4621 cggacggcac cagcatagcc gcttccagta tcagcacatc gtcgcctgct ggcgcttcag
     4681 tgccggcttg cggcacggcc ggaacctcgt cttcgttcaa gatacgccgg atggacgcga
     4741 gaatctcctc catcgaggga tcggcggcgc ttggggtcgg ggcgggattg gatggtttgt
     4801 ctgacattgt ctcgacgatt ttcagcggcc gggctggctg gtagcatagt cgccaagtcc
     4861 aacccagcgg tccttgaccg cctggtagta agcggtctcg tcatatagcg gcaccggcag
     4921 gtgcaggtca cgcgcggtca ggcggccgat cgcgacggca accgcgtagg aggcggtgat
     4981 cacctgcgcc aggttctgca ccagggtggt gcgcgaactc aacagcgcct gggtcgcgtt
     5041 cagcacgtcg agggtcgtgc ggctgccgac gatcgcctct cgttccgtcc cctcaagcgc
     5101 gatctgattg gcgcggatct gcgagcgtgt gctgtcggcc gaagctttgg cggcgaccaa
     5161 cgtgccccag gcctgaaccg cgttctgcac cgcggtgcgt cttgcgtcgt cgatcagacg
     5221 gttggtctgc tgctgctgct gccgagcctg acgcaccgcc gcgtattccg acccaccctg
     5281 gtaaatcggc acgttaagcg atgcggtgac ctgaaagccg ttattcgatg tgctgcggcc
     5341 gccagcattg gtttgctgga aggtctgacc attgagactg acggtgggca gcagggcgga
     5401 cagtgccaga tcgacggcgt ccttcgccgc agcgtcattg aacagcgcgg tgacgacgtt
     5461 cgggttgttg gccgcggcga gtgccgatgc ttcctgctcg ctgttcaccg gcagattgag
     5521 cggttgcggc tcgatcaggt cagaaggggc ggcgaagccg acgaactgct ggaagttacc
     5581 ccgctgggtt tgcagatttc cttcggccgt ttcgcgctgc gcccgggcac cttgcagcgc
     5641 ggcctcggcc tgcgccacgt cggtacgggt gatttcgccg acacggaacc ggtcgttggt
     5701 cgcctgaagc tgtttggaga gcacctgttc gttgttgatc tgcagcgcca gaagctgctg
     5761 agtctggatc accccgacat aggcggcgac cgcattgttg aagctggttt gctcctgtgc
     5821 gagcaaagtt gcccgctccg ccatcacctg attcttcgac cggttgacgc ctgcctgggt
     5881 ctttccgccg gtatagacat tttgcgtaac ggtcgcctga gcggtgccga tgacccggtc
     5941 tgtctgagcg gcgatcacgc gtccaagttg ggccgtgtac gcccgcgaca caccgtcgcc
     6001 gtagccggcc tgtcccccaa ggaccattgt cggccgccac ccggccaggg ccgtcggcac
     6061 gttttcatcg gtcgcccgca gcctcgcgcg ctcggcttgc agtgctggtt gataggtata
     6121 agtcgccgcc agagcctcgg tcagggtgtg cggaatatat tgggcggccg ccgcgcccgc
     6181 tgccacggcg gtacccggtg cggccttgcc tttcggcgcg gcgccctcgg ccgatcgcac
     6241 cgcgggcgcc atggccggag gctgcgacaa cggggacctt gccggtgtct ggcccccggc
     6301 ggcagccgta agggcaacca agctgaagct ggcgagcaaa ccgtgacgca aacccatttg
     6361 atacccttct cagaagaccc ccggccgtgt cggcaggctt aaccaagcat cctgatgatg
     6421 tggttaccac gccaaccgcg gcgatcacca ccaaatccgc tgcattccaa tgtcgttcta
     6481 caagactgcg tcttttctga ctcagccagt tctcgttcgc ccagttctgg ttcgcccagt
     6541 cctaattaag ccagtcctcg ttcgcccgtt tctcgctcct tcgggggcgc cgtcagaacg
     6601 agaaggcggg cgccttccgc aacgacggga tgggcggggt ggcacaatcg aaaatcgatg
     6661 aaaggccgag accgaacgaa gtcatctcgc cgatcgtcgc ctgcgtgacc cggcccgggg
     6721 cgtggatcgc ggttatgatc cggccggtct cctggtgcat ctgggcggcc agggcagggg
     6781 ggatttccgg aaccgcgcct tcgataagga taaggtcgta gggggctccc gacgcccaac
     6841 cggcggcaag gggacccgat accaccgtca cgccgggcgc ttgttccggc agcaccgcat
     6901 tggccatcgc cagcagggcc ggatcttctt ccaacgcggt aacgcggcag ccgcaatccg
     6961 cgagcaccgc ggcgccataa ccagtcgccg cgccgaccac caggacacgt tcgttttcct
     7021 gcagcctggc agcttgcagc agccgggcga acaccatcgg ttccatcatg aagcggccgt
     7081 tgcccagcgg cacgtc
//
LOCUS       DASWQN010000637         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_61318, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000637 DASWQN010000000
VERSION     DASWQN010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61318"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VGC82_10825"
     CDS             <1..596
                     /locus_tag="VGC82_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423806.1"
                     /translation="PWTVAETTGCYVSLILRHQPNRSWPFAFDAKVGYRLSGAALTVT
                     IQLTNRHGKAAPAGIGLHPYFPKLGDPALRFDAGGAWENGADALPRRHGVPRPDWLHT
                     DPRSVARSRLDNCFTAWQGTADILAGSAKLQIVASEVFRQLQVFTPSWADFFCVEPVS
                     HVPGAINRIDLPAVQAMHILQPNEALSGTIHFNHSSG"
     gene            complement(752..1417)
                     /locus_tag="VGC82_10830"
     CDS             complement(752..1417)
                     /locus_tag="VGC82_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY0423807.1"
                     /translation="MGAAADFPHPPPSAVAAIETLIALDPDFSGILERAGALPWRRRA
                     SGFPGLLQAIVAQMISNQAAAAIWGRLRAIPGAMDPALLVGMPDDTLRAAGLSRPKVI
                     HAKALATAFLDGTLSAERLALLDDEAAIGTIGSIKGMGRWTAEIYLLFALDRLDVFPS
                     GDIALAAALADLKAMPVRPPPAALRELAQAWQPVRSLAARLLWHHWRHVTGRPAMDDF
                     KIL"
BASE COUNT          285 a          563 c          484 g          280 t
ORIGIN      
        1 ggccctggac cgtggctgaa accaccgggt gctacgtcag tttgatcctg cgacaccagc
       61 ccaaccggtc ctggcccttc gccttcgatg ccaaggtcgg ttaccgccta tcgggcgcgg
      121 cgctgacggt caccatccag ctgaccaacc ggcacggtaa ggcggcaccg gccggaattg
      181 gcctccatcc ttattttcca aaacttggcg atccggcctt gcgcttcgac gccggcggag
      241 cctgggaaaa tggcgcggat gcactgccgc gacgccatgg cgtgccgcga cccgactggc
      301 tgcacaccga cccccgttcg gttgcccgat cccggttgga caactgcttc accgcttggc
      361 aagggacagc ggacatcctg gctggctccg ccaaactgca aatcgtggcc agcgaagtgt
      421 ttcggcaatt gcaggtgttc acgccatcct gggccgattt cttttgtgtc gaaccggtca
      481 gccatgtgcc gggtgcaatc aaccgcatcg atctgcccgc cgtacaggca atgcatatcc
      541 tacagccgaa cgaagccctc agtgggacga tccacttcaa ccacagttcg gggtgagtcg
      601 ggctgtccca agctccttgc ccgccgcgaa gtctcatctc cgctagaccg ttggtctatc
      661 tccttgtttg gtcgcgtgat tcacacgcat cgtgcgaaca gcgcgtcctt cggacggcgg
      721 acattccgcc cagcgtgatc acgccatagg gtcaaaggat cttgaaatca tccatcgccg
      781 gacggcccgt gacatgcctc caatggtgcc acaacagccg agccgccagt gaccggaccg
      841 gctgccaggc ctgcgccaat tcccgcagcg ccgccggcgg gggccgcacg ggcattgcct
      901 tcagatcggc caatgccgcg gccagcgcga tatcgcccga ggggaacacg tccaaccggt
      961 ccagtgcgaa aagcagatag atctcagcgg tccagcgtcc catgcccttg atggacccaa
     1021 tggtgccgat cgccgcctcg tcatccagca gcgcgagacg ttcggcgctc agcgtgccgt
     1081 ccaggaacgc cgtcgccaac gccttggcat ggatgacctt cgggcgcgac aggccggcgg
     1141 cgcgcagcgt gtcgtccggc attccgacca acagggcggg gtccatggcc cccggtatgg
     1201 cgcgcagccg gccccagatc gccgcggctg cctggttgct gatcatctgt gccacgatcg
     1261 cctgcagcag gccggggaat ccggatgccc gccgccgcca tggcagcgca cccgcacgtt
     1321 cgagaatgcc ggaaaaatcc gggtcgagcg cgatcagcgt ctcgatcgcg gcaaccgcag
     1381 aagggggtgg atgcggaaaa tccgcggctg cccccatggt gtctggctcc atgcggcaga
     1441 ctctggcgtc aaccttcgcc gcgccgcaag cccattaatt gacaccctgt taaccgaaac
     1501 tcgctacccg acgccccgtt ccggacgccc ctacccgact cgggcgatcc tgccggtgtg
     1561 cgggtccatg gtgaacttcg cgaccatcga accctcgggc gtttccaggc tg
//
LOCUS       DASWQN010000638         3028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2172276, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000638 DASWQN010000000
VERSION     DASWQN010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2172276"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VGC82_10835"
     CDS             <1..351
                     /locus_tag="VGC82_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0423808.1"
                     /translation="CVLLVDDDDLVRETLAAQLEDLGFTTLEASSGHDAIAIVEAHEG
                     VDALVCDLAMPDLNGIQTIRAVRHRRPELPCFLLTGYIGEQTALSRSDTFTLLRKPMS
                     GGALADHIEAVLSV"
     gene            complement(508..583)
                     /locus_tag="VGC82_10840"
     tRNA            complement(508..583)
                     /locus_tag="VGC82_10840"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(548..550),aa:Phe,seq:gaa)
     gene            complement(817..999)
                     /gene="yacG"
                     /locus_tag="VGC82_10845"
     CDS             complement(817..999)
                     /gene="yacG"
                     /locus_tag="VGC82_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEY0423809.1"
                     /translation="MAKQPSSCPICGRRSSEANRPFCTPRCQEVDLGRWLTGGYRIPG
                     PPAESDEDPPPSPDAA"
     gene            complement(992..2089)
                     /locus_tag="VGC82_10850"
     CDS             complement(992..2089)
                     /locus_tag="VGC82_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEY0423810.1"
                     /translation="MSVSIRVATSPGEAHVAVVDDNRLLDYALWRPGAPDGVGDVHRG
                     RVIAQVPAMAGAFVALVDAEGFLPDSEGAKGLTAGTMLTVRVTRAAQGNKGPRLTARD
                     ATLTSGPVALIRRGPDPVSRLAARYPEAPIVVDDAGAAASLRLGSRVSIATAVFDEDI
                     AEAIEALSRSVVELRDGASLSIWPTPALVAIDLDAGGALTGGGGARSRHEALNRAALP
                     FLAEQIRLRNLSGGIVVDLAGLSPRKRATLAPNLIDALAIDPLHPRFLGFTALGLAEI
                     VRPRIHPPLHELLAGPLPAGLAALRAVMGEFNRDPRALPAIRAHPAVIGALQADPVAL
                     PDLARRTGRGVTLRSDPSMAATAWVLEKDHG"
     gene            complement(2086..2661)
                     /locus_tag="VGC82_10855"
     CDS             complement(2086..2661)
                     /locus_tag="VGC82_10855"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEY0423811.1"
                     /translation="MKPLPLILASSSPRRMMLLQQIGIVPDHVVSPDIDETPLRNEQP
                     RPYAIRMAWGKATAVTSPDHLVLAADTVVAAGRRILPKAADAAEVRACLTLLSGRRHK
                     VMTAIVLTGPDRRATERLVESAVTFNRLTADQIERYVATREGEGKAGGYAVNGQAAGF
                     IRFLSGSYSGVVGLPLFETAQLLRGHGWQVP"
     gene            complement(2695..2913)
                     /gene="infA"
                     /locus_tag="VGC82_10860"
     CDS             complement(2695..2913)
                     /gene="infA"
                     /locus_tag="VGC82_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY0423812.1"
                     /translation="MSKEDMIEFSGTVMELLPNAMFKVKLDNEHSILAHTSGKMRKNR
                     IRVLAGDRVNVEMTPYDLSKGRITFRFK"
BASE COUNT          521 a         1010 c          995 g          502 t
ORIGIN      
        1 tgcgtgcttc tggtggacga cgatgacctg gttcgcgaga ccctcgcggc gcaacttgag
       61 gatctggggt tcacgacgct cgaagccagc agcgggcacg atgcgatcgc cattgtggaa
      121 gcccacgagg gcgtagatgc actagtctgc gatttggcga tgccggacct gaacggcatc
      181 cagactatcc gcgcggtgcg ccatcgccgg ccggaactgc cgtgctttct gctgaccgga
      241 tatatcggcg aacaaacggc actctcgcga tcggacacgt tcacgctgct acgcaagccg
      301 atgtccggcg gcgcgctggc cgatcatatc gaggctgttc tgtcggtttg aacgcgcgag
      361 tcggtgtcgc atcgcggccg gcgtcgtaca taatcttccg cggtaactgg gacggccaca
      421 cggccggttc cggcagaaaa ctgatcggaa atggcgcctg tcggggctta ggccgagccg
      481 cgatgcactg tgagatgttg aggtctatgg tgcccagggg cggaatcgaa ccaccgacac
      541 tgcaattttc agtcgcatgc tctaccaact gagctacctg ggccacgccg ccgcaaccga
      601 acgacagcga ggcgggtgct taacccatga ccgcccgacg atcaagcgtc gatggccgaa
      661 gcttcgttca ccccgcgtcc ggtgcggata ctcccaccgc gtccggccca tgatgtccac
      721 gccgcgtccg accaatgatg ctcacgccgc gtccgaccaa tgatactccc gccgcgtccg
      781 acccatgatg ctcacgccgc gtccgaccca taatgttcac gccgcgtccg gcgatggcgg
      841 cggatcctcg tcggactcgg ccggcggccc tggaatgcgg taacccccgg tcagccagcg
      901 gccaagatcg acctcctggc agcgcggggt acagaatggc cggttcgcct cggacgatcg
      961 ccggccgcaa atcgggcaac tggatggttg cttagccatg gtccttctcc aatacccagg
     1021 ccgttgccgc catggacggg tccgaccgca atgtaacgcc gcgtccggtg cgccgcgcaa
     1081 gatccggcaa tgcgaccgga tccgcttgca acgcaccgat caccgccggg tgcgcccgga
     1141 tcgccggcaa ggcgcgcggg tcgcgattga attcgcccat caccgcccgc agcgcggcca
     1201 gtccggccgg taatggtccc gccagcaact cgtgcagcgg tggatgaatg cgcggccgaa
     1261 cgatctccgc cagcccaagc gcggtaaagc cgaggaatcg agggtgcagc ggatcgattg
     1321 ccagggcgtc gataagattg ggcgccagcg tcgcccgctt gcgcggtgac agcccggcca
     1381 ggtccacaac aatcccaccc gagagattcc gcaacctgat ctgttcggcc aagaacggca
     1441 gggccgcccg gtttaacgcc tcgtgccgcg accgtgctcc gccgccgcca gtgagggcac
     1501 cgccggcgtc caggtcgatc gcgaccagcg ccggcgtcgg ccagatcgac agcgacgctc
     1561 catcgcgcag ttcgaccacg gagcgtgaaa gcgcctcgat cgcctccgcg atatcctcat
     1621 cgaataccgc cgtcgcgatg ctgacccgcg aacccaggcg caacgacgca gcggcgccgg
     1681 catcgtccac gacaatcggt gcttcgggat accgggcggc gaggcggcta accggatcgg
     1741 ggccgcggcg gatcagcgcg accggtccgc tggtcagggt cgcgtcccgg gcggtcagcc
     1801 gcggaccctt gttgccctgc gccgcccggg tcacccgaac ggtcagcatc gtgccggcgg
     1861 tgaggccctt tgccccctcg ctgtcgggca gaaagccttc tgcgtccacg agtgccacga
     1921 aggcgccggc catggccgga acctgcgcga tcacccgccc gcggtgcacg tcgccgacgc
     1981 catccggggc gccgggacgc cacagcgcgt aatccagcag acggttatcg tccacgacgg
     2041 cgacatgggc ctcgccgggg ctggtggcga cgcggatcga gacactcatg gcacctgcca
     2101 gccgtggcct cgcaacaact gcgccgtctc gaacaagggc aggccgacaa cgccggagta
     2161 gctcccggac aggaagcgga tgaagccagc ggcctggccg ttgacggcat agccgccggc
     2221 cttgccctcg ccctcccgcg tcgcgacgta gcgttcgatc tggtcggctg tgaggcgatt
     2281 gaatgtgacg gcgctctcga ccagccgttc ggtggcgcgc cgatccgggc cggtcagcac
     2341 gattgccgtc atgactttgt gccggcggcc cgaaagcagc gtcaggcagg cgcgaacctc
     2401 ggcggcgtca gcggccttgg gcaggatacg gcgaccggcc gcgaccacgg tatcggccgc
     2461 cagcaccagg tggtcgggcg acgtcaccgc ggtcgccttg ccccaagcca tgcggatggc
     2521 ataggggcgc ggctgctcgt tgcggagggg cgtttcgtcg atgtcggggg agacgacgtg
     2581 atccggcacg ataccgatct gctgcaggag catcattcgg cgggggctgg aggatgccag
     2641 gatcagcggg agcggcttca cccgctgtag tcgtgcatca attggggtcg cacactactt
     2701 gaagcgaaag gtgatgcggc ctttggacag gtcgtacggc gtcatttcca cgttcacccg
     2761 gtcgccagcg agcacgcgga tgcggttctt gcgcatcttg ccgctggtgt gggccagaat
     2821 ggagtgctcg ttatccaact tcaccttaaa catggcgttg ggcaggagct ccatcaccgt
     2881 gccgctaaat tcaatcatgt cttcttttga catcaggcct cttatgtctg ttgctttgac
     2941 cggaacatga tattcagcgg gggccaggtc aagccgtggt tcggccttgc tgccattcgg
     3001 ggccggtcga taacggatag gcttcgcc
//
LOCUS       DASWQN010000639         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1380934, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000639 DASWQN010000000
VERSION     DASWQN010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380934"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VGC82_10865"
     CDS             <1..276
                     /locus_tag="VGC82_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423813.1"
                     /translation="QWGACWLAGQLWEKLHLEQFHGDRKRCRPVRRPTPMYHGRAWRG
                     HPRLPLLPRENPVSSHRETALTWVDITPTDVDLAEQAHIPAQLSLPP"
     gene            complement(387..>2764)
                     /gene="mfd"
                     /locus_tag="VGC82_10870"
     CDS             complement(387..>2764)
                     /gene="mfd"
                     /locus_tag="VGC82_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY0423814.1"
                     /translation="QSGGGPGVTVFDQLRGQAERWNTEGRRIVVAAWTRGSRERLTNL
                     LREHGFKDAAQEDDLASIRRKPAGSVSVIVLGIERGFVAERMALVGEQDLLGERISRP
                     PRRRKRADQFIAEATEIAEGDLVVHQEYGIGRYDGLATLHVTGAPHDCLRLIYDGDEK
                     LFLPVENIEVLSRYGSEHQGATLDKLGGVAWQNRKAKMKQRIRDMAGELIRIAALRKM
                     RDAETMAPPEGTWDEFCSRFPFAETEDQSRAIADVLEDLAAGKPMDRLICGDVGFGKT
                     EVALRAAFVAAMSGSQVAVVVPTTLLARQHFRTFTARFAGMPVRVAQLSRMVTAREAG
                     DVRKGLANGDINIVVGTHALLAKSVEFSDLGLLIVDEEQHFGVAHKERLKAMKADVHV
                     LTLTATPIPRTLQLALTGVREMSIIATPPVDRLAVRTFIMPFDSVVIREALQRERFRG
                     GQVFCVVPRIEDMQRMSDRLKEIVPEAKTIQAHGRLAPTELERVMTEFGDGKYDILLS
                     TNIVESGLDMPAVNTLLIYRADMFGLGQLYQLRGRVGRGKQRGYAYLTWPPNHRLSVA
                     AEKRLTVMQTLDALGAGFTLASHDLDIRGAGNLLGDEQSGHIREVGIELYQQMLEDAV
                     ADIRVEGKRAGQERDWTPNISLGLPVLIPEAYVRDLPVRLGLYRRIGALASDGESDAM
                     AAELVDRFGSMPEEVENLLGVVALKRACREAGVEKLEAGPKGMVLTFRGNAFSNPAGL
                     VRWLSSKGGLVRLRPDHKLAIVRDMDVATRLKFARDTLRSLDQIVRQAKAA"
BASE COUNT          491 a         1009 c          791 g          473 t
ORIGIN      
        1 caatggggcg cgtgctggct ggccgggcag ttgtgggaga aactgcatct agagcagttt
       61 cacggtgacc ggaaaaggtg ccggccggtt cgccgcccga cgccgatgta tcatggccgt
      121 gcttggcgcg gccacccacg acttcctttg ctgccgcgag agaacccagt ttccagtcac
      181 cgtgaaactg ctctaacgtg ggtcgatatc acaccaaccg atgttgatct cgccgaacaa
      241 gcacacatcc cggcgcaact cagccttcct ccgtgacccc tgggtcaaac cttgctttgc
      301 cgcacctccc gcactcaggg gaagaagcac cgcactaccg gccgcgtttc ccaatgaacg
      361 accgcccacc accccgcccg ccagctctaa gccgccttcg cctgccgcac gatctgatcc
      421 aacgaccgca gcgtgtcgcg ggcaaacttc agccgcgtag ccacatccat atcccgcacg
      481 atcgccagtt tatggtccgg ccgcagccgc accaggccgc ccttgctcga taaccaccgc
      541 accagcccgg ccgggttgga gaacgcattg ccacggaagg tcagcaccat gcccttcggg
      601 ccggcctcca gcttttccac cccggcctcc cggcaggccc gcttcagcgc caccacgccc
      661 agcaggtttt ccacctcctc cggcatcgac ccgaaccgat ccaccagttc ggccgccatt
      721 gcgtcgcttt cgccgtccga ggccagcgcg ccgattcggc gataaagccc cagccgcacc
      781 ggcagatcac gaacataggc ttccgggatc aacaccggca gacccagcga gatgttcggc
      841 gtccagtcgc gttcctggcc ggcgcgcttg ccctcgaccc ggatatcggc gaccgcgtcc
      901 tccagcatct gctggtaaag ctcgatcccg acctcgcgga tatggccgga ttgttcatcc
      961 ccaagcaagt tgccggcacc gcggatatcc aggtcgtgcg acgccagggt gaacccggca
     1021 cccaacgcat ccagcgtctg catcaccgtc agccgtttct ccgccgcgac ggatagccgg
     1081 tggttcggcg gccaggtcag ataagcgtag ccgcgctgct tgccccggcc aacccggccg
     1141 cgcaactgat aaagctgccc aagaccaaac atgtccgccc ggtagatcag cagagtattc
     1201 accgccggca tgtccaggcc gctctcgacg atattcgtgg acaacagaat gtcgtatttg
     1261 ccgtcgccga actcggtcat cacccgttcc agttcggttg gcgccagccg cccgtgcgcc
     1321 tggatggtct tcgcttccgg cacgatctcc ttcagccggt cggacatgcg ttgcatgtcc
     1381 tcgatccgcg gcaccacgca gaacacctgg ccgccacgga accgttcgcg ctgcagcgcc
     1441 tcgcggatca ccaccgagtc gaacggcatg atgaacgtgc gcaccgccag gcgatcgact
     1501 ggcggcgtgg cgatgatcga catctcccgc accccggtca gcgcgagctg caatgtccgc
     1561 gggatcggcg tcgcggtcag cgtcagcaca tggacatcgg ccttcatcgc cttcagccgt
     1621 tccttgtgcg cgacgccgaa atgctgctcc tcatcgacga tcagcagccc gaggtcggaa
     1681 aactccaccg acttcgccag caaggcgtgc gtcccgacga cgatattgat gtcgccattg
     1741 gccaaaccct tgcggacgtc gcccgcttcc ctggcggtca ccatccgcga caactgcgcc
     1801 actctcaccg gcattccggc gaaccgagcg gtaaacgtcc ggaaatgttg ccgggcaagc
     1861 agcgttgtcg gcaccaccac cgccacctgg gagcccgaca ttgccgccac gaaggcggcg
     1921 cgcaacgcga cctcggtctt gccgaagccg acatcgccgc agatcagccg atccatcggc
     1981 ttgccggctg ccagatcctc cagcacatcg gcgatggcgc gggactggtc ctcggtttcc
     2041 gcgaacggaa agcgcgagca gaattcgtcc caggtgcctt ccggaggcgc catggtctcg
     2101 gcatcgcgca tcttgcgcag ggcggcgatg cggatcagct cgcccgccat gtcgcggatg
     2161 cgctgcttca tcttggcctt gcgattctgc caggccacac cgcccagttt gtccagcgtg
     2221 gcgccctgat gctcgctgcc gtagcgcgaa aggacctcga tgttctctac tggaagaaac
     2281 agtttttcat cgccgtcgta gatcagccgc aggcagtcgt gcggcgcacc cgtcacatgc
     2341 agcgtcgcca ggccgtcgta gcggccgatg ccgtattcct ggtgcaccac caggtcgccc
     2401 tcggcgattt ccgtcgcttc ggcgatgaac tggtcggccc gcttgcgccg gcgcggcggg
     2461 cgggaaatcc gctcgcccag caagtcctgc tcgccgacca gcgccatgcg ttcggcgacg
     2521 aagccgcgtt cgatacccaa aacgatgacc gagaccgaac cagctggctt gcggcggata
     2581 ctcgcgagat cgtcttcctg cgcggcatcc ttgaagccat gttcccgcag caagttggtc
     2641 agccgttcgc gggacccgcg cgtccatgcg gcaacgacaa ttcgccgccc ctcggtgttc
     2701 caacgctccg cctggccgcg aagctggtcg aagacggtaa cgccaggacc cccacccgac
     2761 tgcg
//
LOCUS       DASWQN010000640         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1511919, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000640 DASWQN010000000
VERSION     DASWQN010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511919"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1524
                     /locus_tag="VGC82_10875"
     CDS             76..1524
                     /locus_tag="VGC82_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423815.1"
                     /translation="METQKLLHEISSPAHAQGATPPVEPMAAPDSAAAVLPPPRRRDP
                     TAPRCGAHIGVMPFRSFGEAPHLALGLADEITNGLSRFRWMFIVSANSLGRFAPENRD
                     EAAIRRELGLDLLLDGSVQQAGSRIRISIRMLDLRASSRIVWARRFDRETDDLLMLQD
                     EIAAEVVAQIDPAVLLIEADRATSRGQADATAYDRVLQSVPLIGRLELEPFLQAGRNL
                     AQAIAQEPDYAAAYAWYAYWHIFLIAQDWTENPKAITQKAMTQKAGELAERSIVLDPL
                     DARGLTISGHVRAFLHRRPREAMVLHDRALSLNPNLAMAWALSAAACAYAGDLTEAER
                     RNNRYKSLSPMDPHAFFFDSFFVLIHLMKRDYATAVELGRNVSLLNPLFSAAYKPYLA
                     ALGHLHRDQEAAATRRRLLALEPDFTIERFLEDSAIERECDRMHYAEGLRLAGVRERD
                     PPTANAPIVLRPSLEHHPAKWDHLPDPDKDAR"
BASE COUNT          312 a          511 c          522 g          297 t
ORIGIN      
        1 gagacaggtc ttgcgttaca agcgtacgac cgctgacagg cggtcctggc ggatttgctg
       61 gacgcgacgc catcggtgga aacccagaag ctgttgcacg aaatcagcag cccagcgcac
      121 gcccagggcg cgacgccgcc ggtggagcct atggcggcgc cagattcggc cgccgcggtg
      181 cttcctccac cacgcagacg ggatccgacg gcaccccgtt gcggcgccca catcggcgtc
      241 atgccgttcc gatcgttcgg cgaagccccg catctggcac taggactggc agacgagatc
      301 acgaacgggc tgtcccggtt ccgttggatg tttatcgtgt ccgccaactc gctcggccgg
      361 ttcgcaccgg aaaaccggga cgaggccgcg atcaggcggg aactcggttt ggacctcctg
      421 ctggacggat cggtgcaaca ggcgggcagc aggatccgca tcagcatccg gatgctggac
      481 ctgcgagcca gcagccggat cgtctgggcc cgccggttcg atcgggaaac cgatgacctg
      541 ttgatgttgc aggatgaaat cgcggcggag gtcgtagcgc agatcgatcc ggcggttctg
      601 ctgatcgagg cggatcgcgc cacaagccgc ggtcaagccg atgccaccgc gtatgaccgc
      661 gtgctgcaat cggtcccgct gatcggccgc ctggaactcg aacccttcct gcaggccggc
      721 cggaacctgg cacaggcgat tgcgcaggag cccgactatg cggcggctta tgcctggtat
      781 gcctattggc acattttcct catcgcacag gactggacag aaaacccaaa ggcgataacg
      841 cagaaggcga tgacgcagaa ggctggcgaa cttgccgaac gctcgatcgt tctcgatccg
      901 ctcgacgcgc ggggcttgac gatcagcggc catgtccggg cgttcctgca tcgtcgtccg
      961 cgggaagcga tggtgctgca tgatcgggcg ttgtcactca atccgaacct ggcgatggcc
     1021 tgggcacttt cagccgctgc ctgtgcttat gccggcgacc tgacggaggc ggaacggcgt
     1081 aacaaccgct acaagtccct gtcgcctatg gacccgcatg cgttcttctt cgactcgttc
     1141 ttcgtgctga tccatttgat gaagcgtgat tatgcgaccg cggtagagct tggccggaat
     1201 gtcagcctgc tcaacccttt gttcagcgca gcttacaagc cttatctggc cgcactggga
     1261 cacctgcatc gtgaccagga ggcggcggca acacggcggc gcctgctggc tctggagccc
     1321 gatttcacca tcgagcgctt cctcgaagac agcgcgattg agcgcgaatg cgacaggatg
     1381 cactacgcgg aaggtttacg cctcgctggg gtgcgggaac gcgatccgcc cacggccaac
     1441 gcaccgatcg tgcttcggcc gagcctggag catcatccgg ccaagtggga tcacctaccg
     1501 gatccggata aagatgctcg ctaaaacaat gatctagact gttttcagtg agcccaggcg
     1561 aacggtctag ccgacagcct gtctgatccg gacaaggtgg cccggataag gcagcagact
     1621 ggcgcggatg ttctccccag gt
//
LOCUS       DASWQN010000641         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1381044, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000641 DASWQN010000000
VERSION     DASWQN010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381044"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VGC82_10880"
     CDS             <1..935
                     /locus_tag="VGC82_10880"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0423816.1"
                     /translation="VRAQRSDRTARIGVLMGIGESDPEAGPRVEALELGFRELGWTKG
                     HNLQLDYRWTAGDLDRARIFAKEIVELKPDLIVVHSTPAVKALRQLTTTIPMVFVLIA
                     DPIGSGFVQNLAHPGGNITGFMNVDSPMTSKWLELIVDIAPKTRQVALIYNPNTASFY
                     QNFLRTFDAAAPKFNVRGIATPVTDAAGIERAMKELGQLPDTAMFVLPDVFVQVHRAL
                     IIKLAEQYRVPAIYPYRFFATSGGLLSYGIDTVIVFRQAAGYADKILKGVTPNDLPVQ
                     APLTFKLTVNLKAAHAIGLSIPESFLLRADEVIE"
     gene            complement(1055..1408)
                     /locus_tag="VGC82_10885"
     CDS             complement(1055..1408)
                     /locus_tag="VGC82_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423817.1"
                     /translation="MTLKGVPAFTMIAIVGLGVQVHAQTPCRELVRLRNTGTEAWKQA
                     MRAPRSERCGALDHAALAADATFKYADNNRVSCGISVPLLNQVEGYHRQAVQARDNVC
                     AGRPLRPFPADIMQR"
     gene            1707..2120
                     /locus_tag="VGC82_10890"
     CDS             1707..2120
                     /locus_tag="VGC82_10890"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0423818.1"
                     /translation="MNSMVEMDDQAVRVALQRHWDASDAGDFEVEHEIYREDAVLDYP
                     QSGERIRTRRNIQESRFVQPNRKRFTVRRMIGGGGLWVTEFVLTYDGIPSYAVSIMEF
                     REGLVANETQYFADRFDPAPSRAHLVERMGEITPF"
BASE COUNT          450 a          639 c          665 g          477 t
ORIGIN      
        1 cggtccgcgc gcagcggtcc gaccggacgg cgcgaattgg tgtcctcatg gggattggtg
       61 aaagcgatcc cgaagcgggg cctcgcgtag aggccttgga actgggtttc cgcgagcttg
      121 gttggacaaa gggccacaac cttcagctgg attatcgttg gaccgcaggg gacttggatc
      181 gagcgcgaat cttcgcaaag gaaattgtcg aactcaagcc cgatcttatt gtcgttcaca
      241 gtaccccggc agttaaggca ttgcgccaac taacgacgac cataccgatg gtcttcgtct
      301 tgatcgcaga cccgatagga agcgggttcg ttcagaacct cgcgcatccc ggaggcaaca
      361 ttacgggctt tatgaatgtc gattctccga tgaccagcaa gtggttggag ttaatcgtcg
      421 atatagcgcc gaagaccaga caagttgcct tgatttataa tcccaatacg gcgtcatttt
      481 atcagaactt ccttcgtaca tttgacgccg ctgcgccgaa attcaatgta cgcggcatcg
      541 ccacgccggt cactgatgcg gctgggatcg aacgcgcgat gaaggaactc ggtcaattgc
      601 cggacacggc aatgttcgtg ctccccgacg ttttcgtgca ggtccaccgc gcgttgatca
      661 taaaactggc ggaacagtat cgcgtgccgg cgatctatcc atatcggttc tttgcaacga
      721 gcggcgggtt gctgtcgtat ggaatcgaca ccgtcatcgt ttttcgccaa gccgctggat
      781 atgcagacaa gatcctgaaa ggtgtgacgc caaatgacct tccggtacag gcgccattga
      841 cgttcaagtt gactgtcaac ctgaaggccg cgcatgcgat tggcctatcg atcccagagt
      901 cgtttcttct tcgtgctgat gaagtgatcg aatgagcgtg ttcacttctg ctattggcac
      961 taagccgaca ccgtgatctc aggcaatgtc cgctttgcgc cagaaccgga ccttccctta
     1021 agtcgggcaa gcactggacc agcttcccgt gatctcagcg ttgcatgata tctgcgggaa
     1081 atggtcgaag agggcggccc gcacagacat tgtcgcgagc ctgtaccgcc tggcggtggt
     1141 agccttccac ctggttcagc aacgggacgg agatgccgca tgacacgcga ttgttatctg
     1201 cgtatttgaa tgtcgcgtcc gcggccaggg cggcatgatc taatgcgccg cagcgctccg
     1261 atcgcggtgc tctcatcgcc tgtttccagg cctctgtgcc cgtgttgcgc agccgcacca
     1321 gttcccggca cggcgtttgc gcgtggacct gaaccccgag cccgacgatg gctatcatgg
     1381 tgaaggccgg cactcctttc agcgtcatag ggttatcctc gcaaagcgtc cgtgccaccg
     1441 gatacgcgac agcataccgc caatattggc ccgtcaggtg cagcggatat gcgaccacgc
     1501 tggttggacc gaacgatgac agccggtcag gccgcttgtc caaatcgagg cgagaagtct
     1561 tcgggccact ggccgatgca atccgcacat tgccggaggc tccgcacagg cgtatcctca
     1621 ataccaaccg caatcgcccg catcgtgagg cggcccacga tcggcgcggt cggcagcgcc
     1681 cacggttcag aatggcgttc acccctatga attcgatggt cgagatggat gatcaggccg
     1741 tgcgggtggc gctgcagcgc cattgggatg cttcggacgc gggcgatttc gaggtcgaac
     1801 acgaaatcta ccgcgaggat gcggtgcttg attatccgca atcgggcgag cggatccgca
     1861 cccggcgcaa cattcaggag agccggttcg tccagccgaa caggaagcgt tttacggtcc
     1921 ggcgaatgat cggcggcggc ggtctctggg tcactgaatt cgtactgacc tatgacggca
     1981 taccgtctta cgcggtaagc atcatggagt tccgcgaagg actggtggcc aacgagacgc
     2041 aatatttcgc cgatcggttt gaccctgctc cctcacgtgc gcatcttgtc gagcgaatgg
     2101 gcgaaatcac gccattctag atgcgatgcc ggtccgggcg gtcagggctc cggcggcgga
     2161 tgcccttatc gtatcgccga agaagttggc tgactgtgaa gtagcttacg tttccgcgtt
     2221 cccggcccgg g
//
LOCUS       DASWQN010000642         4095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_719859, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000642 DASWQN010000000
VERSION     DASWQN010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719859"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1270
                     /locus_tag="VGC82_10895"
     CDS             266..1270
                     /locus_tag="VGC82_10895"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY0423819.1"
                     /translation="MPNVTRRRLGGLAGASILLPRFAHAAEINWKFTSLQPSVHPSTV
                     RYQEAAERVKQKSNGRFEITVFHSGQLGTDIDTFTQVRIGGVHMMVLSNVITATGAPL
                     TSALSIPFSFKNYDQVWAAADGTLGELLREDLKKAGYLTTNKILDSGFHQITNNKGPI
                     NTVDDVQGLKLRAPPSPIQVSTWKSIGAAPASMNFSELYTALQTGVVDGQESPASLAK
                     TTRLYEVQKYMSITAHLWDGWYTLINPRAFGKLPKDMQELVMESFDQAGMDERKDLAV
                     LNQDAQTFMAEKGMLFNTPPDTSSFRKKLKDVGFYTEWKEKLGPDVWNALQDISGPLT
                     "
     gene            1295..3175
                     /locus_tag="VGC82_10900"
     CDS             1295..3175
                     /locus_tag="VGC82_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY0423820.1"
                     /translation="MDASNGPLHAGLPLRATAIARIAGDIERPLIYVMERIAAALVLA
                     DVAVLLLGVIARYVFQHPLIWTDDLAQAVFLWLGMIGSVVALNRGEHMRMTAILARAN
                     RPARAGMEAYGAAVGLMFLAAVVQPSIEYVEEVSRLMMPGLGVSKAWLTAPLPVGFAL
                     MIVIAATRLFRAFPAWVGVVLIALAAAVAWGVPLADPWLESLGNINLLLFFVGLIGAS
                     VLAGVPISIAFAFSTFAYLVLTTDVPMLVFVGRFYEGMSHLILLAVPLFVFLGLLIEM
                     TGMARAMVGFLASLLGHIRGGLSYVLVGAMYLVSGISGSKAADMAAVAPALFPEMIKR
                     GAKPGDLVALLSATGAQTETVPPSLVLITIGSVTGVSISALFTGGLLPSIVLAIALCF
                     VVRYRYRKQDLSGIRRATGSEVGRAFIASVPALALPFVIRFMVVEGVATATEVSTIGI
                     VYTIVIGLLVYRQFDWSRIVPMLVETASLSGAILLILGAATSMAWALTQSGFSQQLAD
                     AMTGLPGGKYSFLGVSILAFVVLGSVLEGIPVIVLFGPLLFPIAQKLGIHEIHYAMVT
                     VLAMGIGLFAPPFGVGFYAAAAIGRVDPNLGIRPIMGYLVAMLIGLIVVAAVPWISIG
                     FL"
     gene            complement(3201..3584)
                     /locus_tag="VGC82_10905"
     CDS             complement(3201..3584)
                     /locus_tag="VGC82_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0423821.1"
                     /translation="MNLLLDTHVFLWWDRRDEALCAEARTAIEDPRNNVFISAASIQE
                     IAIKRRLGKLAFLGSASSAIGANGFHELPVVPLDAEAAGDLAWQHNDPFDRLLVAQAM
                     RLGLILLTADRLVRTFGGIAQIWAG"
     gene            complement(3581..3871)
                     /locus_tag="VGC82_10910"
     CDS             complement(3581..3871)
                     /locus_tag="VGC82_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY0423822.1"
                     /translation="MPNASLSVGQPSQGKIMTQFVSLYDAKTRLSALVDRASAGEEIV
                     IAKNGVPFARLMPIEQRGERRKPANAMRIGYLADDFDAPDPRIEQMFGGDDS"
     gene            complement(3903..>4095)
                     /locus_tag="VGC82_10915"
     CDS             complement(3903..>4095)
                     /locus_tag="VGC82_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423823.1"
                     /translation="GSVEQSDLYGRKGNWDPRHLFYARDGEIRMTPSLGKQMVAHCDR
                     NEINRPVHERRRVKPPQSL"
BASE COUNT          726 a         1215 c         1297 g          857 t
ORIGIN      
        1 tggtcggcac aggcgccggc gttgtggttt cggccggctc ggaaaccacg gccaaccggt
       61 gaagcgagag actccctgca ccgtgtgcag gcgagcgcca cccgaagcgt ggctggaagc
      121 agtaagcctt aaacaggctt ggagccctgc cgcacagcca ctggcgcaca gccgctgtcg
      181 caccaccact gtcgcaccgc cacatgacaa gctcgaccgc gtgagctagc atttgtccaa
      241 tgacaaacgg ggagaaacga tagacatgcc gaacgtcacc aggcgccggc tcggtgggct
      301 ggccggagcc tcgatcctgc tgccccgctt cgcccatgcg gccgagatca actggaaatt
      361 caccagcttg cagccatccg ttcacccatc cacggtgcgc tatcaggagg ccgccgaacg
      421 ggtcaaacaa aaatccaacg gacggtttga aatcaccgtt ttccattccg gccagctggg
      481 caccgatatc gacacgttca cgcaagtgcg catcggtggc gtgcatatga tggtactgtc
      541 gaacgtcatc accgcaaccg gtgcgccgtt gacctcggca ttgagcatcc ccttcagctt
      601 taagaattac gatcaggtct gggctgctgc cgatggcacg ttaggcgagt tgttgcgcga
      661 ggacctgaaa aaggccggct acctgaccac caacaagatc ttggacagcg gcttccacca
      721 gatcaccaac aacaagggac cgatcaacac cgtcgatgac gtccagggat tgaagctgcg
      781 tgcgccacca agcccgatcc aggtttccac ctggaaatcg atcggtgcgg ctccggcctc
      841 gatgaatttc tctgagcttt acaccgcgct gcaaaccggc gtcgtggacg gtcaggaaag
      901 ccccgcttct ttggccaaga ccacccgcct gtatgaagtg cagaaataca tgtccatcac
      961 cgcccatttg tgggacggct ggtacacgct gattaatccg cgcgccttcg gcaagcttcc
     1021 aaaggatatg caggagctgg tgatggaaag cttcgatcag gcagggatgg acgagcgtaa
     1081 ggatctggcg gtactgaacc aggacgcgca aaccttcatg gccgagaagg gcatgctctt
     1141 caacaccccg cccgacacct cgtcgttccg caagaagctg aaggacgtgg gcttctacac
     1201 cgagtggaaa gagaagctgg ggcctgacgt gtggaatgcg ctgcaggaca tctccggccc
     1261 gttgacctga ttgccgccgc acggagaccc ggtcatggac gccagcaacg gcccgcttca
     1321 cgcgggcctg ccccttcgcg cgaccgctat cgctcggatc gccggggata tcgaacgacc
     1381 gctgatctac gtgatggagc gcatcgccgc ggcactcgtg ctggccgacg tggccgtcct
     1441 gctgctgggc gtcatcgcgc gctacgtgtt ccagcatccg cttatctgga ccgacgacct
     1501 ggctcaggcg gtgttcttgt ggctgggcat gatcggttcc gtcgtggcgc tgaaccgcgg
     1561 cgaacacatg cgaatgacgg cgatactcgc ccgcgccaac cgccctgcac gcgctggaat
     1621 ggaagcatac ggtgcggctg tgggcttgat gttcctggcg gccgtggtgc agccctcgat
     1681 cgagtacgtc gaggaggttt cccgcctgat gatgccgggg ttgggcgtgt cgaaagcgtg
     1741 gctgacggca ccgctgccgg tcggtttcgc gctgatgatc gtcatagcgg caacacggtt
     1801 gttcagggcg tttccggctt gggtcggcgt ggtgctgata gcattggccg cggcggtcgc
     1861 ctggggcgtg ccgctggccg acccgtggct ggagtcgctc ggcaacatca atcttctgtt
     1921 gttcttcgtg ggactgatcg gagcatcggt cctggcgggg gtgccaatct ccatcgcctt
     1981 cgcgttctcc acgttcgcct acctggtatt gaccacggat gtgccgatgc tggtgttcgt
     2041 gggccgcttc tacgagggca tgtcgcacct gatcctgctg gcggtgccgt tgttcgtgtt
     2101 cctgggtctg ttgatcgaaa tgaccggtat ggcgcgggcg atggtcggct tcctggcctc
     2161 gctgctgggg cacatccgcg gcggactgtc ctatgtgctg gtcggcgcga tgtacctagt
     2221 gtccgggatt tccggatcga aggccgccga catggcggcg gtggccccgg cgctgtttcc
     2281 ggaaatgatc aagcgcggcg ccaaacccgg ggatcttgtg gcactgctgt cggccaccgg
     2341 cgcacagact gaaacggtgc ccccgagcct ggtgctgatc accattgggt cggtgacggg
     2401 ggtatcgatt tcggcgctgt ttactggtgg gttgctgcct agcatcgtgc tggccatcgc
     2461 gctgtgcttc gtggtgcgct atcgctatcg caagcaggat ctgtcgggca tccggcgcgc
     2521 gacaggatca gaagttggac gcgccttcat cgcctcggtg ccggcgctgg cactgccttt
     2581 cgtcatccgg ttcatggtgg tggagggcgt ggcgaccgca accgaggttt ctactatcgg
     2641 cattgtctat actatcgtca tcggcctgct ggtgtaccgg cagttcgact ggtcgcgcat
     2701 cgtgccgatg ctggtggaga cggcgtcgtt gtccggcgct atcctgctga tcctgggtgc
     2761 cgcgacctcc atggcctggg cgctgacgca gtcgggtttc tcgcaacagt tggcggacgc
     2821 gatgaccggg ttgccgggcg gcaagtacag ctttctgggc gtgtcgatcc tcgctttcgt
     2881 cgtgctgggt tcggttctcg agggcattcc cgtcatcgtg ctgttcgggc cgttgctatt
     2941 cccgatcgcg cagaaactag gcatccacga gatccactac gcgatggtga ccgtgctggc
     3001 gatgggtatc ggattgttcg cgccgccatt cggagtggga ttctacgccg cggcagcgat
     3061 tggccgggtg gaccccaatc tggggatacg gccgattatg gggtatttgg tggcgatgct
     3121 gatcggcttg atcgtcgtgg cggctgtgcc gtggatttcg atcgggtttt tgtagggcgg
     3181 cgtggaacct gatcgaagcc tcatccagcc caaatttgcg ctataccgcc gaaagtgcgg
     3241 accaggcggt ccgcggtcag caggataagg ccaaggcgca tcgcctgggc caccaagagc
     3301 cggtcaaacg gatcgttatg ctgccaggca aggtcgcccg cggcttcggc atccagcggc
     3361 acgaccggta gttcgtggaa accattggcg ccgatcgcgg acgatgccga accgagaaac
     3421 gctagtttcc ccagtcggcg cttgattgcg atctcctgaa ttgaggcggc gctgatgaag
     3481 acgttgttgc gcggatcttc aatcgcggtg cgggcttccg cacacaaggc ttcgtcccgc
     3541 ctgtcccacc aaaggaaaac atgggtatcc agcaaaagat tcacgaatca tcgccaccga
     3601 acatttgctc gatgcgagga tcgggtgcgt caaagtcatc agccagatag ccaatgcgca
     3661 tggcattggc gggttttcgc cgctcgccgc gctgttcgat tggcatgagc cgagcgaaag
     3721 ggacaccgtt cttggcgatc acgatttcct cgccagccga agcgcggtcc acgagcgcgg
     3781 agagccgcgt cttcgcatcg tataggctga cgaactgggt catgatctta ccttgactag
     3841 gttgaccaac cgatagcgat gcgttgggca atagtcaatg actcgatgcg tactggctgc
     3901 gatcacagac tttggggcgg tttcactcgc ctccgctcat ggaccggccg attgatttcg
     3961 ttgcggtcgc aatgtgccac catctgtttt ccgaggcttg gcgtcatcct gatttcaccg
     4021 tcccgtgcat aaaataaatg tcgcggatcc caattgcctt tccggccgta caggtccgat
     4081 tgttccaccg agccg
//
LOCUS       DASWQN010000643         3449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1381126, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000643 DASWQN010000000
VERSION     DASWQN010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381126"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VGC82_10920"
     CDS             complement(<1..242)
                     /locus_tag="VGC82_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0423824.1"
                     /translation="MMLQVRDLHAYYGQSHILHGIDLDVGEGEIVSLLGRNGVGRSTT
                     CKAIMGLVPPQGRITFRGRELAGLRCDMVAKAGIGYV"
     gene            complement(239..985)
                     /locus_tag="VGC82_10925"
     CDS             complement(239..985)
                     /locus_tag="VGC82_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0423825.1"
                     /translation="MTALSLRDVRKNFGATEIIRGVTLDIAKGERHCIIGPNGAGKTT
                     CFNLISGRFPITSGEILLNDQRIDALPPHSINRLGLSRSFQITSIFHRLSVFENLRCS
                     LLWSRGYRYSFWSPLWRQKALNEAAEALLEDLNLATRHDVLAGLLSYAEQRALEIGMT
                     IAGGASVVLLDEPTAGMSNTETDYAMALIRKVTQGKTLLMVEHDMKVVFDLADTITVL
                     VYGQVIASGPPAAVRANRAVQEAYLGALEG"
     gene            complement(982..2235)
                     /locus_tag="VGC82_10930"
     CDS             complement(982..2235)
                     /locus_tag="VGC82_10930"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0423826.1"
                     /translation="MKSRWLIAALIVAAIALPWLFYDWGHHRHAGFLISMLSQMGMMI
                     ILALSYNMLLGQAGLFSLCHATFFGIGGYAMIHMLNAAGDGGLPVPMEAMPVLAGLAG
                     LGLAIVFGAMATKQRATAFAMITLGISELMATAALMFHHFFGGEGGVTTNRMIENSVF
                     GLTYASGIQVYYLTLTWTVIAIALMYYLTLTPLGRMANATRDNFERAQFIGYDPRMVR
                     FLQFALSGFFAGIGGGLYAIIYEIVTFDALAAPLSANALLMAYIGGITVFGGPILGAI
                     LITLLQSGVSLLSNSWLVYVGVMFIAMVIFAPTGLTGIINAHRPIKRAERLGYLIMPY
                     LRLVIPSLGVLLGFIGLVELMSFLTIGAAQGKRLVLFGNTIDVHATLPWVVSGGSLLV
                     GGLWLRFEAAGFHRVWESLTAEARR"
     gene            complement(2232..3179)
                     /locus_tag="VGC82_10935"
     CDS             complement(2232..3179)
                     /locus_tag="VGC82_10935"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0423827.1"
                     /translation="METILISVLNGLVYGMLLFMLASGLTLIFSMMGVLNFAHASIYM
                     VGAYLAFTISQVVGFFPALIIAPIVCGLIGAAIEIWGLRRVHHNGHIAELLFTFGLVF
                     IIQRLVQMTWGMLPMAFRVPASLDFPLFVLYGVNFPAYRGFMLLVSGAMLLGTWLLLK
                     KTRIGLIIQAALTHPGMVGALGHNVPRVFTTVFAGGCLLAGLAGVIGGNYQTTEPGMA
                     DSMGPIVFVVVVFGGLGSLAGCFIASILMGMIQTFAVVIDYSLSDLLHPFGLTLSGDT
                     LLTEILTVPVARIGALLPLVMMVTILFFRPRGLLGTRDT"
     gene            complement(3195..>3449)
                     /locus_tag="VGC82_10940"
     CDS             complement(3195..>3449)
                     /locus_tag="VGC82_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423828.1"
                     /translation="KAGSTDALKVALALEDMKATDIVDAPVQMRKEDHQLLMPYYAVS
                     FTKDVKYDAEKTGLGWKTDFVATTEDLTLPTTCKMKRPTS"
BASE COUNT          752 a         1065 c         1059 g          573 t
ORIGIN      
        1 acgtagccga tcccggcctt ggcaaccatg tcgcaccgca ggccagcaag ctcgcggccg
       61 cggaaggtga tccgcccctg gggtgggacc agccccatga tcgccttaca ggtggtggac
      121 cggccgacgc cattgcggcc aagtaaggag acgatttcgc cttcgcccac atccaggtca
      181 atgccgtgca ggatatggct ctgcccgtag taggcgtgca ggtcccggac ttgcagcatc
      241 atccttccag cgcccccaga taggcttcct gcaccgctcg gtttgcccgc acagcggccg
      301 gcggaccaga ggcgatgacc tggccataga ccagcaccgt aatggtatcc gcgaggtcga
      361 acacaacttt catgtcgtgt tccaccatca gcagggtctt gccctgcgtg accttgcgaa
      421 tcaacgccat ggcatagtcg gtttcggtgt tgctcatgcc cgccgtcggt tcgtcgagca
      481 ggaccacgga ggcgccgccg gcgatggtca taccgatctc cagcgcgcgc tgttcggcat
      541 aggatagcag acccgccaac acgtcgtgac gggtggccag gttcaggtct tccagcaagg
      601 cttcggccgc ctcgttcaag gctttttgcc gccacagcgg cgaccagaac gagtaacggt
      661 agccgcggct ccagagcagg gagcagcgca gattctcgaa caccgacagg cggtggaaga
      721 tcgaggtgat ctgaaagctg cgcgacaaac caagccggtt gatgctgtgc ggcggcaggg
      781 cgtcgatccg ctggtcgttc agcaggatct cgcctgaagt gatcggaaag cgcccgctga
      841 tcaggttgaa gcaggtcgtc ttgccggcgc cgttcggacc aatgatgcag tgccgttcgc
      901 ctttggcgat gtccagggtg acgccgcgga tgatttccgt cgcgccgaaa ttcttgcgga
      961 cgtcgcgcag ggacagcgcg gtcaccggcg ggcctcggcg gtcaggcttt cccagacccg
     1021 atgaaagccg gccgcttcga aacggagcca caagccaccc accagcagcg acccgccgga
     1081 gaccacccag ggcagcgtcg catgcacgtc gatggtatta ccgaacaaca ccagtctttt
     1141 tccttgcgcc gccccgatgg tcaggaacga catcaattcc accagtccga taaagcccag
     1201 caaaacaccg agcgatggaa tgaccagccg cagatacggc atgatcagat agccaagccg
     1261 ttcggcccgc ttgatggggc ggtgcgcgtt gatgatgccg gtcaggccgg tgggcgcgaa
     1321 gatcaccatg gcgatgaaca tgacaccgac atagaccagc caggagttcg acagcaggct
     1381 gacgccgctc tgcagcaggg tgatcaggat cgcgccgagg atcgggccgc cgaacacggt
     1441 aatgccgccg atataggcca tcagcagcgc attagccgac aagggtgcgg ccagcgcgtc
     1501 gaacgttaca atttcgtata taatagcgta aagtccgccg ccgatgccgg cgaagaaacc
     1561 ggacaaggcg aactgcagga accgcaccat cctcgggtca tacccgatga actgcgcccg
     1621 ctcgaagttg tctctggtgg cgttggccat gcggccgagc ggcgtgagcg tcaggtagta
     1681 catcagcgcg atggcgatga cggtccaggt cagggtcagg tagtaaacct ggatgccgga
     1741 ggcataggtc aggccgaaaa cactgttttc aatcatgcgg ttggtcgtaa cgccgccctc
     1801 gccaccgaaa aaatggtgga acatcagggc ggcggtggcc atcaattcgc tgatgcccag
     1861 ggtgatcatg gcgaacgcgg tcgcgcgctg cttggtcgcc atcgcgccga acacgatcgc
     1921 caggccaaga ccggccaggc cggccagaac cggcatcgcc tccatcggca ccggcagacc
     1981 gccgtctccg gctgcgttca gcatgtggat catcgcgtac ccaccgatgc cgaaaaacgt
     2041 cgcgtggcac agggagaaca ggcccgcctg ccccagcagc atgttgtacg acagtgccag
     2101 gatgatcatc atgcccatct gactgagcat ggaaatcaga aagccggcgt ggcggtgatg
     2161 tccccagtcg tagaacaacc aaggcagtgc gatcgcggcg acaatcaggg ctgcaatcag
     2221 ccagcgggac ttcatgtgtc gcgggtccct agcaggccgc gaggccggaa gaacaggata
     2281 gtgaccatca tcaccaacgg cagcagcgcg ccgatgcggg cgaccgggac ggtcaggatc
     2341 tcggtcagca gcgtgtcgcc ggacagcgtc aggccgaacg ggtggagcag atcggacagc
     2401 gagtagtcga ttaccacggc gaaggtctgg atcatcccca tgaggatcga ggcgatgaag
     2461 cagccggcga gcgagcccaa tccgccgaat accaccacca cgaaaacgat cgggcccatc
     2521 gagtcggcca tgcctggttc cgtggtctgg tagttgccgc cgatcacgcc ggccagcccc
     2581 gccaaaaggc aaccgccggc gaaaaccgtg gtgaagacgc gtgggacgtt atgaccgagc
     2641 gcgccgacca tgccgggatg tgtcaaggcc gcctggatga tcagaccgat gcgggtcttt
     2701 ttcagcagca gccatgtgcc cagcagcatc gcgccggaca ccagcagcat gaagcctcga
     2761 taggccggaa aattgacgcc atagagcacg aacaacggga aatccagcga cgcaggcacc
     2821 cggaatgcca tcggcagcat accccaggtc atctgcacca ggcgctggat gatgaacacc
     2881 agcccgaagg tgaacagaag ttcggcaatg tgcccgttgt gatggacgcg tcgcagcccc
     2941 cagatctcga tcgccgcgcc gatcagcccg cacacgatcg gagcgatgat cagcgccgga
     3001 aagaatccca cgacctggct gatggtgaac gcgagatagg cacccaccat gtagatactg
     3061 gcatgggcga agttcagcac acccatcatg ctgaagatca gtgtcaagcc gctggcgagc
     3121 atgaacagca gcatgccgta caccaagccg ttcagcaccg agatcagtat cgtttccata
     3181 aaccaaaggt ccggctagga tgtcggccgc ttcatcttac aggtcgtggg aagcgtcaga
     3241 tcctcggttg tcgcgacgaa gtcggtcttc cagcccaggc cggttttttc cgcatcgtat
     3301 ttgacgtcct tggtaaagct gactgcataa tacggcatca gaagctggtg gtcttccttt
     3361 cgcatctgca ccggcgcatc cacgatgtcg gttgctttca tatcctccag cgccagcgcg
     3421 accttcaaag catcagtcga tccagcctt
//
LOCUS       DASWQN010000644         4462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1776795, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000644 DASWQN010000000
VERSION     DASWQN010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776795"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VGC82_10945"
     CDS             <1..541
                     /locus_tag="VGC82_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423829.1"
                     /translation="TSDIIAENERVPVCGGPQRLLDTFGFSSRRKRTAITDRVNPIDL
                     PPIVHPSPDWRCAILPTETDDAARLAAARQKRAGHLKVLLLSHIDRRRLVGGALITAG
                     TSIAAAALIFAYATAPSPYGHIRDVVQPLAGSVVFGQLTSFGFSQVGLPLRYVMARIL
                     FNMEIYQRWLRQRNYAYWR"
     gene            910..1266
                     /locus_tag="VGC82_10950"
     CDS             910..1266
                     /locus_tag="VGC82_10950"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY0423830.1"
                     /translation="MGYCHCRSCRSWSAAPVNTFSLWSPEAVEVVRGKEHIAAYHKTD
                     LSHRQFCRLCGGHLMTQHPPLKMIDVFAATIPALAFMPQIHVNYAETVLPMHDGLPKL
                     RDFPAEFGGSGEVVAE"
     gene            complement(1204..1404)
                     /locus_tag="VGC82_10955"
     CDS             complement(1204..1404)
                     /locus_tag="VGC82_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423831.1"
                     /translation="MAIGMSSCSSKIEHGLLDDHRLFDLIRKGHRHFRGKLARPLLDL
                     LALFRNNLAGTAKLRREIPQLG"
     gene            1397..2797
                     /locus_tag="VGC82_10960"
     CDS             1397..2797
                     /locus_tag="VGC82_10960"
                     /inference="COORDINATES: protein motif:HMM:NF017505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HEY0423832.1"
                     /translation="MAMRPDPTFHASPKLAMEAEPENFAYTVLLSPDFSKPDALAVID
                     VKPGSPTYSQIVHTVTMPYKGDEFHHFGWNACSSSLSPLTGHAFLERRYLIIPGIRSS
                     RIYVVDTKPHPTQAKIHKIIEPEEVFRKTGYSRPHTVHCGPEGIYVSTLGGGGKDGTD
                     GPPGVFIMDCETFDILGRWEIDRGPQVLHYDFWWNLPRDYMVTSEWGLPPQFENGIVP
                     DDLLSGKYGHHIHFWDLRARRNVQTIDLGANHQMALEIRPAHDPVREYGFLGVVVDNT
                     NLEGSIWTWWREGGKFHIEKTATIPPEPAPKDQLPPLLQGFGAVPPLVTDIDLSMDDK
                     FLYVACWGTGEMRQYDVTDPRKPKLAGSVHTGGIVRRTPHPNGKMFVGGPQMVELSRD
                     GKRVYWTNSLYSTWDNQFYPDGLPGAQVMAIANPNGGLALERDYWVTFPDGYRAHQIR
                     LEGGDCSTDSFCYPSA"
     gene            2865..3536
                     /locus_tag="VGC82_10965"
     CDS             2865..3536
                     /locus_tag="VGC82_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423833.1"
                     /translation="MNPEAVWAPAGLWLAVVASGLYHGINPGMGWPLAVSAGLMEKNS
                     RALVAAFWPLTVGHFLAMLLVILPFSLLVTLVYWQQQIQIGASLLVIGFGTFRFVYRR
                     HPRALARIRPTQLGLWSFTVALAHGAGLMLVPIYLGLCQITNAASRPIGGRVSADLRT
                     AILVSVFHSAAMLAGGGVSAWLVYRYLGLKFVSRGWFNLDAVWAISLVLVGTVSLALG
                     LKASY"
     gene            3681..3839
                     /locus_tag="VGC82_10970"
     CDS             3681..3839
                     /locus_tag="VGC82_10970"
                     /inference="COORDINATES: protein motif:HMM:NF042577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6726 family protein"
                     /protein_id="HEY0423834.1"
                     /translation="MRLALCLIVLASLGGCGVVALPFRVTGDVVQAVPVIGKPIGKPI
                     HAVGDAID"
     gene            4160..4399
                     /locus_tag="VGC82_10975"
     CDS             4160..4399
                     /locus_tag="VGC82_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY0423835.1"
                     /translation="MRSNAAVFRKVHEPLTIEAVDIDKPQGREVLVRTVATGVCHSDL
                     HVVDGLSRYPTCPSCWAMKEPAWWRRLAATSPLCG"
BASE COUNT          826 a         1392 c         1357 g          887 t
ORIGIN      
        1 tacaagcgac atcatcgcag aaaacgagcg cgtgccggtg tgcggtgggc cacagcgttt
       61 actcgatact ttcggctttt cgtcgcgccg aaagaggacg gcaataaccg atcgagttaa
      121 cccaatcgac ctcccgccca tcgtccatcc gtctccggac tggcgttgcg cgatcctgcc
      181 caccgagacg gatgacgccg cccgcctggc cgccgcgcgt cagaaacgcg ccggtcattt
      241 gaaagtgctt ctcctatcgc acatcgatcg ccggcggctt gtaggcggag cgttgatcac
      301 agccggcacg tcgattgccg cagccgcact gatcttcgca tacgcgacgg cgccttcccc
      361 gtatggccac atccgcgatg tggtgcagcc gttggccggc tccgtcgtgt tcgggcaact
      421 caccagtttt gggttctccc aggtcggttt gcccctgcgg tacgtgatgg cccgtatctt
      481 gttcaacatg gagatttacc agagatggct acggcaaagg aactacgcgt actggcgatg
      541 accctccgcg cctgggcaag aagaattcat gatccaggta cggcaaagcg cacagtcggt
      601 cttgcagccg aaatggacgg actggcggcg agtatcgaag tagccgaccg gcaattggtg
      661 tgaccagcaa aagcgtagga caggctgtcc ggccgtaacg ttgactggta acgcccttat
      721 cgccggtgag ccttcaattc gctgtcccca ctgcgacagg ccacccaact gcgacaggtt
      781 ggcgcggcgg tcccaacgct tggcaaccca gcgtcaacag taacaggaga aactagcatg
      841 agcatcgtat accaaggcac atgtttttgc ggtgccgtcg aaatcgcggt tgccggagaa
      901 cccaaggcaa tgggttattg ccattgccga tcctgccgat cgtggtccgc cgcgccggta
      961 aacacttttt ccctatggtc gccggaggcg gtggaggtgg taaggggaaa ggagcacatc
     1021 gccgcgtatc acaaaacgga cctgagccac cgtcaattct gtcgcctctg cggcggacat
     1081 ctgatgactc agcacccgcc actgaagatg atcgatgtct tcgcagcaac catccctgcc
     1141 cttgctttca tgccacagat tcatgtcaac tacgctgaaa cagtgttgcc gatgcatgac
     1201 ggactaccca agctgaggga tttcccggcg gagtttggcg gttccggcga ggttgttgcg
     1261 gaataacgcg agcaggtcca gcagcggtct ggccaacttg cctcggaagt gccggtggcc
     1321 cttgcggatc aggtcaaaga gccgatgatc gtcgagcaac ccatgttcga tcttgctgga
     1381 acaggaggac atcccaatgg ccatgcgacc tgatccgact tttcacgcat cgccaaagct
     1441 tgcgatggaa gccgaaccgg agaacttcgc ctacactgtg ctgctcagcc cggacttctc
     1501 gaaacccgac gccctcgcgg tgatcgacgt gaagcccggc tcgcccactt acagccaaat
     1561 cgtccacact gttacgatgc catacaaggg cgacgagttc catcatttcg gctggaatgc
     1621 gtgctcgtcg tcgttgtcgc cgctcaccgg ccacgccttc ctcgagcggc gctatctcat
     1681 aattcccggt atccgctcct cgcgcatcta tgtggtggat accaagcccc acccgaccca
     1741 ggcgaagatc cataagatca tcgagccgga ggaggtgttc aggaagaccg gctattcacg
     1801 tccccacacc gttcactgtg gcccggaagg catctatgtc agcaccctcg gcggcggcgg
     1861 caaggacggc accgatggac cgccgggcgt cttcatcatg gactgcgaaa ccttcgatat
     1921 cctcggcagg tgggagatcg accgcggtcc gcaagtgctg cactacgatt tctggtggaa
     1981 cctgccgcgc gactacatgg tgacaagcga gtgggggttg ccgccgcagt tcgagaacgg
     2041 gatcgttccg gacgatctgc tctcgggcaa gtatggccat catatccact tctgggatct
     2101 tcgcgcccgg cgcaacgtcc agaccatcga cctgggcgcc aatcaccaga tggcactcga
     2161 aatacggccc gcacatgatc cggtgcgtga gtacgggttc cttggtgtcg tggtggacaa
     2221 caccaatctt gaaggctcga tctggacgtg gtggcgcgaa ggcgggaaat tccacatcga
     2281 gaagacggcc acgatcccgc cggagccagc ccccaaggac cagttgccgc ccctgctgca
     2341 aggattcggc gcggtgccgc cgctggtaac cgacatcgac ctctccatgg acgataaatt
     2401 cctttacgtc gcctgctggg gaaccggcga gatgcgccaa tacgacgtta ccgacccgag
     2461 aaagccgaag ctcgcaggct cagttcatac cggcgggatc gtgcggcgca cgccgcaccc
     2521 aaacggcaag atgttcgtgg gcgggccgca gatggtcgaa ctcagccgcg acggcaagcg
     2581 ggtttactgg accaactcgc tctattccac ctgggacaac caattttatc ccgatggcct
     2641 tccgggcgcc caggtcatgg cgatcgccaa cccaaacggt ggcctggcat tggaaagaga
     2701 ctactgggtg acgttccctg acggctaccg ggcgcatcag atccgtctcg agggcggcga
     2761 ctgctcgacg gattcatttt gctatccgtc ggcttaagca ggcccgtcgg cttaagcagg
     2821 cccgtcggct taagcaggcc catcggctta agcgggccat cggcttgaac cccgaagccg
     2881 tctgggcacc tgccggactg tggctggccg tcgttgcgag cggcctctac cacgggatta
     2941 atcccgggat ggggtggccc ctcgccgtct cggccggact gatggaaaag aattcgcgtg
     3001 ccctcgtggc ggctttctgg ccgctcacgg ttggccactt cctcgcgatg cttctggtca
     3061 tcctgccctt ctcgctgctg gtcacgctgg tttactggca gcagcagatc cagatcggcg
     3121 ccagtcttct ggtcatcggg tttggtacct tccggtttgt ctatcgccgg catccgcgag
     3181 cgctggcgcg gatacggccg acgcaactgg ggctgtggtc gttcaccgtg gcgctcgcgc
     3241 acggcgcggg cttgatgctc gtaccgatct acctcggact ctgccagata accaatgccg
     3301 cgagccggcc gatcggcggc cgtgtgagtg ccgatctccg cacggccatc ctggtctccg
     3361 tcttccactc agcagcgatg cttgcgggcg gtggcgtatc ggcttggctg gtgtaccggt
     3421 acttggggct gaagttcgtg tcgcgcggct ggttcaacct ggacgcggtc tgggccatca
     3481 gcctcgtttt ggtcggcact gtgtcgttgg ccctcggcct gaaggcctca tactgagcgc
     3541 cgggactggc gcatccatca tggccccgga catgggtctt gtcggcgtgc tcatttcgca
     3601 gatggctgaa cgcgtcggcc gcgcgcacca acttgatccg gcggtgcgag cgcaatacat
     3661 ttcggccagg aggtatcacg atgcggttag ccttgtgcct gattgttctc gcttcgctgg
     3721 gcggctgcgg cgtcgtggcg cttcccttcc gggtaactgg tgacgtggtg caggcggtgc
     3781 cggtgattgg caagccgatc ggcaaaccga tccatgcggt cggcgacgca atcgactgaa
     3841 gcagtcggcg caatcccccg acgtgggaaa gcacacaccc tgcatcccgc cgaaccgtat
     3901 gcttcgccgg atgcgggcgc gctgcttgga gcgaaatccg tgctgataaa cacggcttcc
     3961 actttcgccc ttgtttcacg agcatctttg tccggtaggt gatcccactt ggtcggatga
     4021 tgttgcaagc tgctggctca cgcggcgttg ccaccggaac gagaggcccg catcgacgac
     4081 gcccggccta cacggctaat caaggcgtcc gctgccacgg cggaacaaga gcttaatcaa
     4141 ccagggggaa acgatcatca tgcggagcaa cgccgccgtc tttcgcaagg tccacgaacc
     4201 actgaccatc gaggccgtgg atatcgacaa gcctcagggc cgcgaggtgc tggtccgcac
     4261 cgttgccacc ggtgtctgtc acagcgatct gcatgtcgtg gacggcttga gccgctaccc
     4321 gacctgccct tcgtgctggg ccatgaagga gccggcgtgg tggaggcggt tggcggcgac
     4381 gtcacctcta tgcgggtagg cgatcatgtc gttgcctgtc tgtcgggctt ctgcggcaca
     4441 tgcccgcaat gtctcagcgg cc
//
LOCUS       DASWQN010000645         5045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2040678, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000645 DASWQN010000000
VERSION     DASWQN010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040678"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VGC82_10980"
     CDS             <1..272
                     /locus_tag="VGC82_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0423836.1"
                     /translation="HQLRADLVLLAMGFLGPRRGALLDQAGVELDQRGNVLANTVQYQ
                     TSRERLFTAGDMRRGQSLVVWAIREGRQCARAVDVALMGNSTLPR"
     gene            450..1208
                     /locus_tag="VGC82_10985"
     CDS             450..1208
                     /locus_tag="VGC82_10985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0423837.1"
                     /translation="MSFYRGMTCENVTLNGDKGAPITAYVARPSGPGPFPGVVLIHHL
                     PGWSELYIETTRRFAHHGYLAICANLYEREGGGSDGNPDDVAAKVRAEGGIADSQMVG
                     DTEAAVKWMRAQPDNNGKVGLIGSCSGGRHAFIYACQKHDIDAVVEQWGGGVVMAKEE
                     LNAKKPVAPIDMTKDLSCPLLGLFGNDDRAPSPEQVNQHEAELKKYGKTYEFHRYDGA
                     GHGFFYWHRPLYRPEQAMDGWNKIWTFFGKYLAK"
     gene            1220..1525
                     /locus_tag="VGC82_10990"
     CDS             1220..1525
                     /locus_tag="VGC82_10990"
                     /inference="COORDINATES: protein motif:HMM:NF039404.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HEY0423838.1"
                     /translation="MCTSIIEIARAEGMAKRGDEWFPLSHAVVAYDHARHAPLGDVIT
                     LDFINTDLDPGARAGIELTLETAKELRAALDQAINAAEFEEAEVRGKGAVPHLSRAA"
     gene            1599..2822
                     /locus_tag="VGC82_10995"
     CDS             1599..2822
                     /locus_tag="VGC82_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423839.1"
                     /translation="MPERLDLSARGGTKPSAPVGLELGQESAPLQTASRGFDHPRCWD
                     AGGAGQRSGVKTVPCGTQKWIMDDERAFTCTLRQPLLRPPLLLPTLAQFFQQERPNMP
                     QSQGTRFTADPLTFIVRHQLWDGNVEDHSDQGVSIDVAADVGGKQTALLRFNCFDIER
                     SYTYGPENPELTTAGRVGGGMGVHCRMDPITDGNPISWTIQILGKKLPKMLERAGYKE
                     IAEATDLASVCRVLPDVEACARDTFLSKRNTVKHNRGTDIFEAGNIRFGLEMRRQRNG
                     DGGLAIHVLGDVGGSKGKTYVEETELLAFDCFWNGAHYHYGPRNKNHRINWDMTIVED
                     PLEWTFEQLENRKLGAMIERAGYPGIAADLDLDKVASVLPALKKRAFAMYEEGERLTG
                     HKGLPLEFTPNLAAE"
     gene            complement(2936..3859)
                     /locus_tag="VGC82_11000"
     CDS             complement(2936..3859)
                     /locus_tag="VGC82_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY0423840.1"
                     /translation="MTADLLCMGEPMLEFNQLPPNPDGTQHYLEGHGGDTSNAAIAAA
                     RQGASVGMITALGEDMPGDSFIRLWQREGVDTACAMRTDRYQTGVYFVTHDGTGHHFL
                     HYRANSAAAMYSAADVPAQAIAAARILYVSGISQGISTSACDAVFAAIETAISHGVKV
                     AYDTNYRPRLWPPARAAAVMHAAMARADFAFPGEEDVRTLTGLTDPDRMLDFYLRLGS
                     KIVLLKMGEAGAYLGATDVRIRIPPHIVVPVDATGAGDTFCGSFLARILAGDAPEPAA
                     RYASVAAALKCRGYGAVGPIPTAAEVWAAFS"
     gene            complement(3856..4476)
                     /locus_tag="VGC82_11005"
     CDS             complement(3856..4476)
                     /locus_tag="VGC82_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY0423841.1"
                     /translation="MSQSALIQKLRAARVVPVVRTSSAAHAETAVQWLRAAGIRIFEI
                     TMTIPDAIPLLARLAKLPDLLIGAGTVPDEATARACLDAGARFLVAPWVDATLSGPCR
                     QANAALMLGALTPTEVRAALAAGADVVKIFPASSAGGPGHIKALASVFPGVAFCPTGG
                     VEPANVAAYLSAGAAFVGMGGALLDEKRIAAGDRAAIEAVAKAVLS"
     gene            4811..>5045
                     /locus_tag="VGC82_11010"
     CDS             4811..>5045
                     /locus_tag="VGC82_11010"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461702.1"
                     /note="Catalyzes the cycloisomerization of
                     cis,cis-muconate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEY0423842.1"
                     /translation="MPVNPADSPILGTLYGSDAMRAVFDETTYFQRMLDVEAALARVQ
                     GHMGIIPADAAEAIGVAAVVRNLRTEELAASARN"
BASE COUNT          980 a         1563 c         1654 g          848 t
ORIGIN      
        1 agcaccaact ccgggctgat ctcgttttac tcgcaatggg tttcctcggc ccccgccgtg
       61 gtgccttgct ggatcaggcc ggcgtcgaac tggaccagcg cggcaatgtc ctggccaata
      121 ccgtccagta tcagacctcc cgggagcggc tgtttaccgc cggcgatatg cgccgcggcc
      181 aatccctggt ggtgtgggcg atccgcgaag gccgccaatg tgctcgggcg gtggacgtcg
      241 cgctgatggg caacagcacg ctgccaagat aaaggccagg cgtagccggg accagcagat
      301 gccggctcct tgttgcgccc cgcaggcgat tgtcgaatac tgcccgtgcg acgcttttcg
      361 gcagtaggcg agtttatccg cttgtcgatg acggcgcggc gctttgatgg ccgatggcgg
      421 cccaagaccc agaccatgga ggaagatcga tgtcattcta ccgcggcatg acctgcgaaa
      481 acgtaacatt aaacggtgac aagggagcgc cgataacggc ttacgtggcc aggccatctg
      541 gtcccggccc tttccccggc gtcgtgctta tccaccatct tccaggctgg agcgagctgt
      601 acatcgagac gacgcgtcgg ttcgcgcatc acggctatct cgcgatctgc gccaaccttt
      661 atgagcgtga gggtggcggc agcgacggca atcccgacga cgtcgccgcc aaggtgcgcg
      721 ccgagggcgg cattgccgac tcccagatgg tcggcgatac cgaagccgcc gtgaagtgga
      781 tgcgcgcgca gcccgataac aacggcaagg tcggtctcat cggctcctgc tcgggcggcc
      841 ggcacgcctt catctatgct tgccagaagc acgacatcga tgccgtcgtc gagcagtggg
      901 gcggcggcgt agtgatggcc aaggaggagc tcaacgcgaa aaagcccgtc gccccgatcg
      961 acatgacgaa ggacctgagc tgcccgctgc tcggcctgtt cggcaacgac gaccgcgcac
     1021 cgagccccga gcaggttaac cagcacgagg ccgagctcaa gaagtacggc aagacgtatg
     1081 agttccaccg ctacgatggg gccggccacg gtttcttcta ctggcaccgg ccgctatacc
     1141 ggcccgagca ggcaatggac ggctggaaca agatttggac cttcttcggc aagtatctgg
     1201 cgaagtagga gggccggaca tgtgcacgtc catcatcgaa atcgcgcgtg ccgagggcat
     1261 ggcgaagcgc ggggacgaat ggttcccgct atcgcatgcg gtggttgcct atgaccacgc
     1321 gcgccatgcg ccactgggcg acgtcatcac gctcgatttc atcaacacgg acctcgaccc
     1381 gggcgcgcgg gcggggatcg agctgacgct ggagacggcc aaggagctgc gcgccgctct
     1441 tgaccaagcg atcaacgccg cggagttcga ggaagcggaa gtgcggggca agggcgccgt
     1501 gccccacctt tcacgggctg cgtgatgtca aaggcaagtc agccgaagta acttcggctg
     1561 ggcttgcctc tggcgtctgg ttggctgcgg atccgctttt gcccgagcgg ctggatctat
     1621 ccgcgcgcgg tgggacgaag ccctctgccc cggtgggttt agaactcggt caagagtcgg
     1681 cgccgttgca aaccgcgagc cggggcttcg atcatccgcg ctgttgggat gccggcggcg
     1741 cggggcaaag atccggtgtg aagaccgtgc cctgcggcac gcagaagtgg ataatggacg
     1801 atgaacgggc atttacctgc actctgcgcc aaccactctt gcgaccaccg cttctcctcc
     1861 ctacgctcgc acaattcttc caacaggagc gtcccaacat gccacaatca cagggaacaa
     1921 gatttaccgc cgacccgctg actttcatcg tcaggcacca actttgggat ggcaacgtcg
     1981 aggatcattc ggatcaggga gtatccatcg acgtggcagc cgacgttggc ggcaagcaga
     2041 ccgcgctgct gcgcttcaac tgcttcgaca tcgagagaag ctacacctac ggcccagaga
     2101 atccagaact gaccactgct ggccgtgtcg gtggcggcat gggcgtacac tgccggatgg
     2161 acccgatcac cgatggcaat cccatcagct ggaccatcca aatccttggc aaaaaactcc
     2221 cgaagatgct ggaacgggcc ggatacaagg aaatcgcgga ggcaaccgac cttgcttcgg
     2281 tatgccgcgt cctgcctgac gtagaggcct gtgcccggga cacattcctg tccaagcgaa
     2341 acaccgtcaa acacaaccgt ggcacggaca tcttcgaagc cggcaacatc cggttcggcc
     2401 tggagatgcg gcggcagcgg aacggtgacg gtggtctcgc gatccacgtt cttggcgacg
     2461 tgggcgggtc caaaggaaag acctacgtcg aagaaacgga acttctggca tttgactgtt
     2521 tttggaacgg tgcgcactac cactatggcc cgcgcaacaa aaatcaccgg atcaattggg
     2581 acatgacaat cgtcgaagat ccgctggagt ggaccttcga gcagctcgag aacagaaagc
     2641 ttggtgcgat gatcgagcgc gccggttatc cgggaatcgc cgccgacctg gatctggata
     2701 aagtggcctc ggtccttccg gcattgaaga agcgagcctt cgccatgtac gaggaaggcg
     2761 agagattaac cggtcacaag ggattgccgc tcgagttcac cccgaatctg gccgccgaat
     2821 agctcaccgc gaaggcggtg gagggctcag aaaccctcca tcgccgcatt caatgacgcc
     2881 cacctcaggc acggcaaggc gacagctggt gctgcaagat ggcttcgcac gcggtctagg
     2941 aaaaagccgc ccacacctct gccgccgttg gaatcggccc gaccgcgccg tatccccggc
     3001 acttcaatgc cgcggccacg ctggcgtaac gcgccgcggg ttccggcgca tcgcccgcga
     3061 gaatccgggc aagaaacgat ccacagaacg tgtcgcctgc gccggttgcg tccaccggca
     3121 cgacgatgtg cggcgggatg cgaatccgaa cgtcggttgc gcccagatag gcgccggcct
     3181 cgcccatctt cagcagcacg attttcgatc ccagccgcag gtagaaatcc agcatccggt
     3241 cgggatcggt caggccggta agggtccgca cgtcctcctc gccggggaag gcaaagtcgg
     3301 cgcgtgccat ggcggcatgc atcaccgccg ccgcccgtgc cgggggccac agcctgggac
     3361 gatagttggt gtcgtaggcg acctttacgc cgtggctgat cgcggtctcg atcgcggcga
     3421 aaaccgcgtc gcaggccgag gtggaaatcc cctggctgat ccccgagaca tacaggatgc
     3481 gcgctgcggc aatcgcctgc gccgggacat ccgcggccga atacatcgcc gccgcggagt
     3541 ttgcccggta gtgcaggaaa tgatgccccg tcccgtcatg ggtcacgaaa tatacgccgg
     3601 tctggtagcg gtcggtgcgc atcgcgcagg cggtatcgac gccctcgcgt tgccacaggc
     3661 ggataaagct gtcgccgggc atgtcctcgc ccagggcggt gatcattccg acgctggcac
     3721 cttggcgggc ggcggcgatg gcggcgttgg aggtgtcgcc accgtggccc tcgaggtagt
     3781 gctgggtgcc gtccgggttg ggcggaagct ggttgaactc cagcatcggc tcgcccatgc
     3841 acagcagatc ggcggtcatg acagaacggc cttcgccacc gcctcgatcg ccgcccgatc
     3901 gccggccgcg atgcgctttt catcgagcaa ggcgccgccc atcccgacaa acgcggcacc
     3961 ggcggacaag taggcggcca cgttggcggg ctccacgccg ccggtggggc agaacgcgac
     4021 gccagggaag acgctggcca gtgccttgat gtggcctgga ccgcccgcgg aggatgcggg
     4081 gaagatcttc accacgtcgg cacccgcggc cagggccgcg cgcacctcgg ttggcgtcag
     4141 ggcccccagc atcagggcgg cgttggcctg acggcagggt ccggatagag ttgcatcgac
     4201 ccaaggggcg accaggaagc gcgcgccggc atccaggcag gctcgggcgg ttgcctcgtc
     4261 agggacggtt ccggcgccga tcaacaggtc agggagtttg gccaggcggg cgagcagtgg
     4321 aatggcgtcg gggatcgtca tcgtaatctc gaatatgcgg atgccggcgg cgcggagcca
     4381 ttgcacggcg gtttcagcgt gcgcggcgga actggttcgc acgacgggta cgacacgagc
     4441 cgctcgcagt ttttggatca aggcggattg tgacatttgg cgggctcctc aacacataac
     4501 gaaacaggtt gagaaaccag agcgcaagat cgcagccgca ggttgtttcg tgtgtgaaca
     4561 aaatttaccg tggatttccg aatcgtgcgg tcggccgtac tctagctaaa gcggttatac
     4621 ggtgactgga aacaggggtc tctggcggct gcaaaggaag tcgtgggtgg ccgtgccaag
     4681 cacggccatg acacatggac gtcaggcggc gaactggccg gcacattttc ccgatcggat
     4741 tccgaaggag atgttgccgc cgccgcggca ctctggctta aagcctgacg cgccatcagg
     4801 agtgaccgca atgcccgtca atcccgccga cagtccgatc ctgggcacgc tctatggcag
     4861 cgacgccatg cgcgcggtgt tcgacgagac cacctatttc cagcgcatgc tggacgtgga
     4921 agcggcactc gcccgggtgc aggggcacat gggcatcatc ccagccgacg cggccgaggc
     4981 gattggcgtt gcggccgtgg tcaggaatct tcggacggag gagcttgccg ccagcgcccg
     5041 gaatg
//
LOCUS       DASWQN010000646         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2040921, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000646 DASWQN010000000
VERSION     DASWQN010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040921"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="VGC82_11015"
     CDS             complement(<1..162)
                     /locus_tag="VGC82_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0423843.1"
                     /translation="MLTRSLFVRLEAKPGQEDQVAAFLRQGLQLAQEETTTPLWFALR
                     LGPSTFAIFD"
     gene            complement(305..1132)
                     /locus_tag="VGC82_11020"
                     /pseudo
     CDS             complement(305..1132)
                     /locus_tag="VGC82_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
     gene            1204..>1791
                     /locus_tag="VGC82_11025"
     CDS             1204..>1791
                     /locus_tag="VGC82_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY0423844.1"
                     /translation="MRIDVLALDDVFDLGLAAVLDAFQTANELIAVTKMAIPRFDVRM
                     VGVRRSVNTAQGLRVPVHAAGKRTPDCVVVPAIGFKMPGPLEAALARPDVRDAAVALR
                     QWAGQGSIMTAACIGTFVMAESGLLDHRQATTTWWLAPLFRKRYPSVALSDSSMIVKS
                     GRFVTAGAALSHMDLALWLVRGVSPELASLTAKYLI"
BASE COUNT          303 a          569 c          583 g          336 t
ORIGIN      
        1 gtcgaagatc gcaaatgtgc tgggacccaa ccgcaacgcg aaccacaacg gcgtggtcgt
       61 ttcttcttga gcgagctgga gtccctggcg caggaacgcc gcgacctggt cctcctgacc
      121 gggctttgcc tccaaccgaa cgaacaagct tcgtgtgagc actgcggact cctttgagaa
      181 acgtcgtttc cgtaccttct gcggcgagtt gctggccaag cgtgtggaga tacttcatcc
      241 cggtgtcgca catgagggtg accacggtcg ccgccggacc gaggtgttct gccagccgca
      301 gcgccgcgac cacattggcg ccggtggaca caccggcaaa caggccttct tcgcgtgcga
      361 gacgcaatga catggccgtc gcggcttgcg tcgagacctg ctcgatccga tcgacgatct
      421 cgggacgcca cagcggcacg acaaatccag caccgatgcc atcgatcttg tgagcgccgg
      481 ccgatccgcc tgagagcacc ggcgactcgg agggttcgac cgccgtgacg cgaacgtttt
      541 tgtcgtaccg ccgaagcgct tcggcggtgc cgcgcagcga cgccgccgtg ccgacacttt
      601 gcacgaagcc gtcgatccgg ccctcggtct gcgtccagat ttcgtccccc atctgctgat
      661 aggcggccat ctgatcggtg ttgcggagct ggtcggtcca gaacgcgccg gtgtctgcgg
      721 caatctggcg tgcggcctcc accatctcgt tggtgagctg ctcggtcatc cgtccgctgt
      781 cgctgtgcac gacctggagg cgcgcgccaa ggatgcgcat gtgatcgagc ttctctcgcg
      841 agaacgcgtc ggaggtgacg atgtgcagcg gatatcgttt gacggcgcac acgagggcca
      901 acgacacgcc ggtgctgccg ccggtatatt cgacgaccga accgccggcc ggaagccgtc
      961 catcggcttc tgcggcctcg atcatggcga ccgccatgcg gtccttcatg ctgccggtgg
     1021 gattctggtt ctccagcctg agcaggacgc gggcaccgtt cgagggaacg acgtaacgca
     1081 gcggttgtaa cgacgtgtga ccgaccaacc ggaacatgtc tgacgacgcc atggatcaca
     1141 gcctaggcgc aggcgccctt tggcggcaga ggcaagaacg ccaactttga tatgcttctt
     1201 gccatgcgga tcgacgtgct cgccctcgac gacgtcttcg atctcggatt ggcggcagta
     1261 ttggatgcgt ttcagacggc caacgaactg atcgccgtca cgaaaatggc catcccgcgt
     1321 tttgacgttc ggatggtggg ggtgcgaaga agcgtcaaca cggcacaggg cctgcgcgtc
     1381 ccggtgcatg cggccggcaa gcgaaccccc gattgcgtcg tcgtgccggc cattggcttc
     1441 aagatgccgg gtccgctgga ggcggccctg gcgcgtcccg acgtgcgtga tgccgccgtc
     1501 gcgctccgac aatgggcagg ccagggctcg atcatgaccg ccgcgtgcat cggcacgttc
     1561 gtgatggccg agtccgggct tcttgatcat cgacaagcga cgacgacgtg gtggctggcg
     1621 ccgctctttc gaaagcgata cccgagtgtc gcgctgagcg attcgagcat gatcgtcaag
     1681 tcggggcggt tcgtgaccgc cggggccgcc ctgagccaca tggatctcgc gttgtggctg
     1741 gtgcgcggtg tcagtccgga gctcgcgtcc ttgaccgcga agtacctcat c
//
LOCUS       DASWQN010000647         5185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1645157, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000647 DASWQN010000000
VERSION     DASWQN010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645157"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VGC82_11030"
     CDS             complement(<1..835)
                     /locus_tag="VGC82_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY0423845.1"
                     /translation="MRAINAIRMGGGDVLPLVEGGKGVSISNGISAGNWALAGGVGTF
                     SAVNADSFDIDGRAIPQVYYGRSRRERHEELVQYAVQGALTQARRAWDITSGKGRIHA
                     NILWEMGGAERIITEVLEQAKGIINGITCGAGMPYRLSDIAARFNVHYYPIISSARAF
                     NALWKRAYSRTAELLGGVVYEDPWLAGGHNGLSNGEDPNRPEDPFPRVLALRKVMRDF
                     GLHETPIIMAGGVWCLEDWEGWIDNPDLGPVAFQFGTRPLLTKESPIGDAWKQRLLTL
                     KE"
     gene            complement(963..3206)
                     /gene="pnp"
                     /locus_tag="VGC82_11035"
     CDS             complement(963..3206)
                     /gene="pnp"
                     /locus_tag="VGC82_11035"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY0423846.1"
                     /translation="MFNYFRKELDWGGRKLVLETGKIARQADGAVMVSYGETIVLCTA
                     VGVRSAKPGQDFFPLTVNYQEKAFAAGKIPGGFFTREGRPSEAEVLKSRLIDRPIRPL
                     FPEGFRNEVQIVATVLSYDNENDPDIVAMVGCSAALTLSGIPFFGPIAAARVGYRDGQ
                     YILNPTRDEMTTSELELVLAGTAEGVLMVESEARELSEEIMLGAVTFGHAAFQPVINA
                     IIELAEHAAKEPWPLPEKSADELALAGRVDALGRAGIAEAYLEKIKQARHEKVGAAKK
                     AVAAALAAEGLDPEKAKGLLHDLEADVVRNAILETGIRIDGRDTRTVRPIIAEVGILP
                     RAHGSALFTRGETQAFCVATLGTGQDEQVVDALLGEYREHFMLHYNFPPYSVGEAGRM
                     GSPGRREVGHGKLAWRAVHPLLPPKEQFPYTMRVVSEITESNGSSSMATVCGTSLALM
                     DAGVPLKRPVAGIAMGLIKEDRGFAVLSDILGDEDHLGDMDFKVAGTEEGVTSLQMDI
                     KITSITFDIMKTALEQAKEGRLHILGEMAKALTGARGDVAQTAPRITIINVPKEKIRE
                     IIGTGGKVIREIVEQTKCKVDIDDDGTIKIAAVDSEQAQKAIDWIRGIVAEPEIGVIY
                     NGKVVKTADFGAFVNFLGSKDGLVHISELTQGRVGKTTDVVNNGDAVKVKVIGFDDRG
                     KVKLSMRVVDQATGADITEQVGAKPSRGGDRPERGDRPERGDRPERRDRFERADADGE
                     QPSGFRD"
     gene            complement(3565..3834)
                     /gene="rpsO"
                     /locus_tag="VGC82_11040"
     CDS             complement(3565..3834)
                     /gene="rpsO"
                     /locus_tag="VGC82_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY0423847.1"
                     /translation="MSLTAERRTTLIGDYRRAETDTGSPEVQVALLSERIGTLTEHLK
                     IHAKDFHSRRGLLMLVGRRRRLLDYLKGKDQARYQTLIGRLSLRR"
     gene            complement(3877..4803)
                     /gene="truB"
                     /locus_tag="VGC82_11045"
     CDS             complement(3877..4803)
                     /gene="truB"
                     /locus_tag="VGC82_11045"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY0423848.1"
                     /translation="MSRRKPRGRPLDGWLIIDKPAGMTSTDVVNRVKRLFDAQKAGHG
                     GTLDPLATGLLPVAFGAATKTVPYVMDGTKLYHFTLKFGEARDTDDADGAVIQTSDVR
                     PDDAAIEAALPAFRGAIMQVPPIFSAIKVAGERAYDMAREGRIPVLEPRPARVDRFVM
                     IARVDADTAIFEVASGKGVYMRSLARDLARACGSVGYIAALRRLRVGPFLEHHAIPLD
                     KLREPDDTAPASPDLLLPVLTALADIPALALTEQEAAGLKHGQAISLVALMGRIPATA
                     DPDGGLARAMAGGRVIGLCRLQDGMLKPERVL"
     gene            complement(4874..>5185)
                     /locus_tag="VGC82_11050"
     CDS             complement(4874..>5185)
                     /locus_tag="VGC82_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423849.1"
                     /translation="QNHIGDLEVQPLPTQIPALSNPVDLFPGMSKKWGLSFLINTEVG
                     HAGRSAGSLAWAGLNNTYYWLDPVKKVAGVLMTQTLPFADPTVLEALDAFERAVYETA
                     G"
BASE COUNT          871 a         1764 c         1598 g          952 t
ORIGIN      
        1 cctctttcaa cgtcagcagc cgctgcttcc acgcgtcgcc gatcgggctt tccttggtca
       61 gcaaaggccg cgtgccgaac tggaacgcga ccggacccag gtcagggttg tcgatccagc
      121 cctcccagtc ctccaggcac catacgcccc cggccatgat gatcggcgtc tcgtgcaggc
      181 cgaaatcgcg catcaccttg cgcagcgcca acacccgggg gaacggatct tcgggtcggt
      241 tcggatcctc gccgttcgaa agaccattgt ggccgcccgc aagccatggg tcctcgtaaa
      301 ccaccccgcc cagcaactcc gcggtccttg aataggcccg tttccacagc gcgttgaacg
      361 ctcgggccga cgatatgatc gggtagtagt gaacattgaa cctggcggcg atgtcggaca
      421 gccggtacgg catgccggca ccgcaggtga tgccgttgat gatgcccttc gcctgctcca
      481 gcacctcggt gatgatgcgt tcggcgccac ccatctccca caggatgttg gcgtggatac
      541 ggcccttgcc gctggtgata tcccaggcgc gccgagcctg ggtcaatgcg ccctgaacgg
      601 cgtattgcac cagttcctcg tggcgctccc gccgcgatcg gccataataa acctgcggga
      661 tcgcgcgccc gtcaatgtcg aaactatcgg cgttgaccgc gctgaacgtg ccgacaccgc
      721 cggccaaagc ccagtttccg gcggagatgc cattggagat ggagacacct ttgccgcctt
      781 ccaccagcgg caggacgtcg cccccgccca ttcgtatcgc gttgatcgct cgcatggcca
      841 aatgccccct gaaaggccag tccctcgaga agcctatggg attggtctta tccccatgac
      901 cccgtgcccc tccgggggcg ttctaccggc gaaccgatag aacgccgctg gcggatgcaa
      961 gttcagtcgc ggaaaccgga cggctgctcg ccgtcggcgt cggcccgctc gaagcggtcc
     1021 cgccgctccg gacgatcgcc ccgctccgga cggtcacccc gttccggacg gtcgcctccg
     1081 cgcgagggct tcgcaccgac ctgttcggtg atatcggcac ccgtcgcctg atcgacaacc
     1141 cgcatcgaca gcttcacctt gccgcggtcg tcaaaaccga tgaccttgac cttcacagcg
     1201 tcgccgttgt tgaccacgtc ggtggtcttg ccaacccggc cttgcgtcag ctcgctgatg
     1261 tgaaccagcc cgtccttgga ccccaggaag ttcacgaagg cgccgaaatc ggccgtcttc
     1321 acgaccttgc cgttatagat cacgccgatc tccggctcgg ccacgatgcc gcggatccag
     1381 tcgatcgcct tttgtgcctg ttcggagtcg acggccgcga tcttgatggt gccgtcgtcg
     1441 tcgatgtcga ccttgcactt cgtctgctcg acgatctccc ggatgacctt gccgccggtg
     1501 ccgatgatct cccggatctt ctccttcggc acgttgatga tggtgatcct gggtgcggtt
     1561 tgcgccacat cgccgcgcgc gccggtcagt gccttggcca tctcgcccag gatgtgcagg
     1621 cggccttcct tcgcctgctc cagcgccgtc ttcatgatgt cgaacgtgat ggacgtgatc
     1681 ttgatgtcca tctgcaggct ggtcacacct tcctcggtgc ccgccacctt gaagtccatg
     1741 tcgcccaaat ggtcttcatc gcccaggatg tccgacagca cggcaaagcc gcggtcttcc
     1801 ttgatcagcc ccatggcgat gccggccacc ggccgcttca gcggcacgcc cgcgtccatc
     1861 agcgccagcg aggtgccgca aaccgtcgcc atcgaggacg aaccgttcga ctcggtgatc
     1921 tcggagacca ctcgcatggt gtacgggaac tgttccttcg gcggcagcag gggatgcacc
     1981 gcacgccacg ccagcttgcc gtggccgact tcgcgccggc ccggcgaacc catccgtccg
     2041 gcctcaccaa cggagtacgg agggaagttg taatgcagca tgaagtgctc gcggtattcg
     2101 cccagcagcg cgtcaaccac ctgctcgtcc tggccggtgc caagcgtggc aacgcagaag
     2161 gcctgggtct cgccacgggt gaacagggcg gaaccatgcg cacgcggcag aatgccgacc
     2221 tcggcgatga tcgggcggac ggttctcgtg tcacgcccgt cgatccggat gccggtctcc
     2281 agaatggcgt tgcggaccac gtcggcctcc aggtcgtgca gcaagccctt ggccttctcc
     2341 gggtcgagcc cctcggcggc cagcgcggcg gcgacggcct tcttcgcggc accgaccttt
     2401 tcgtgacgcg cctgcttgat tttttccagg tacgcttcgg cgatgccggc gcggcccagc
     2461 gcgtccaccc gcccagccaa ggccagttcg tcggcagatt tctccggcag cggccagggt
     2521 tctttagcgg cgtgttcggc aagttcgatg atcgcattga tcaccggctg gaaggccgca
     2581 tgaccgaacg tcaccgcccc cagcatgatc tcttccgaaa gttcccgagc ctccgactcg
     2641 accatcagca cgccctcggc ggtgccggcg agaaccagct caagctcgct ggtggtcatc
     2701 tcgtcccggg tcgggttcaa gatatactgg ccgtcccggt agccgacccg ggcggccgcg
     2761 atcgggccaa agaacgggat gccggacagc gtcagcgccg cggagcagcc gaccatcgcg
     2821 acgatgtcgg ggtcgttctc gttgtcgtag ctcagcaccg tggcgacgat ctgaacctcg
     2881 ttgcggaacc cttcggggaa caacggacgg atcggtcggt cgatcaagcg gcttttcagc
     2941 acctcggctt cgctcgggcg gccctcgcgc gtgaagaagc caccagggat cttgccggcg
     3001 gcgaatgcct tttcctggta attgacggtc agcgggaaga aatcctggcc cggcttggcc
     3061 gatctcacgc caacggcggt gcaaagcacg atggtctcac cgtaggagac catgacggcg
     3121 ccatcagcct ggcgggcgat cttgccggtc tccaggacca gcttgcggcc gccccagtcg
     3181 agttccttgc ggaaataatt gaacattcag tcgtccttca gatgcgcggc atcggcagac
     3241 gagcagacgg ctgttcccgc cagtccctgt tgtgctcggg gtggcggcgt ctcgggtaac
     3301 acggagcgcg cactgcgtgc cgttggaagg gtcgcagccg cggccatgtg gccggaaacg
     3361 ccgtcgtttg tcccgcgcct ttcggggatg gccggatcgg cgatctttct gcccaccgcg
     3421 tcgcagtgtg ccgggggaat acctgccccc gtccaggtct ttcgatcagt caacatgcgg
     3481 gaaatagtga tgactcccgc ctctgaccag ctttatctac cgttcaggca cgccaacgcg
     3541 gacggacctg gcgccgatcg gttgctaccg ccgcaggctc aggcggccga tcagcgtctg
     3601 atagcgcgcc tgatccttgc ctttcagata gtccagcaac cgccgccgcc gccccaccag
     3661 catcaggaga ccacggcgcg aatggaaatc cttcgcatgg atcttcaaat gttcggtcag
     3721 cgtgccgatc cgttcggaca gcagcgctac ctggacttcg ggactgccgg tatcggtctc
     3781 agcccttctg taatcaccaa taagcgtcgt acgacgctcc gccgttagcg acatcaggca
     3841 actcctgtgc aagagggaag aaaacgcgtg ccgggatcaa agaacccgtt cgggcttcag
     3901 catgccgtcc tgaaggcggc acagcccaat cacgcggccc cccgccatgg ctcgggccaa
     3961 cccaccatcg ggatcggccg tcgcgggaat ccggcccatc aacgcaacca ggctgattgc
     4021 ctggccatgt ttcaggccgg cggcctcctg ttcggtcaag gccagcgccg ggatgtcggc
     4081 cagcgcggtc agaaccggaa gcagaaggtc cggggaagct ggcgcggtat cgtccggttc
     4141 gcgcagtttg tccagcggta ttgcgtggtg ctccaggaaa ggcccaaccc gcaggcgcct
     4201 cagcgcggcg atatagccga cggaaccgca ggcgcgggcc aggtcccgtg ccaggcttcg
     4261 catatagacg cccttacccg aggcgacctc gaagatcgcc gtgtcggcgt ccacccgcgc
     4321 gatcatgacg aaccggtcca cccgtgccgg acgcggttcc agaaccggga tgcggccttc
     4381 gcgtgccatg tcgtaggccc gctcgcctgc gaccttgatg gctgagaaaa tcggcggaac
     4441 ctgcatgatc gcgccgcgga acgccggcag cgccgcttcg atcgcggcat cgtcggggcg
     4501 gacgtcgctg gtctggatga ccgcgccatc agcatcgtcg gtgtcccgcg cctcgccgaa
     4561 cttcagggtg aagtggtaca gcttggtccc gtccatgaca tacggaacgg tcttggtcgc
     4621 cgcgccgaaa gccaccggca gcaatccagt cgccaacggg tccagcgtgc cgccgtggcc
     4681 ggccttctgg gcgtcgaaca gccgcttgac gcggttgacc acgtccgtgc tggtcatccc
     4741 ggccggcttg tcaatgatca gccacccgtc gagcgggcgg ccacggggct tgcggcggga
     4801 catttggctt tctcaggcgt agggcaggcg gcggggtagg gactgaatgc ctggttgtca
     4861 atcactccca gacttatccg gcagtctcgt acaccgcgcg ctcgaacgca tccagcgcct
     4921 ccagcaccgt cggatcggcg aacggcaggg tctgggtcat caacacgccc gcgaccttct
     4981 tcaccgggtc gagccaatag taggtgttgt tcaagcccgc ccatgccagg ctgccggcgc
     5041 tgcgaccggc gtgaccgact tcggtgttga tcaggaaact cagtccccat ttcttcgaca
     5101 tgccgggaaa caggtcaacg ggattggaca gcgccgggat ctgcgtcggc aacggctgca
     5161 cctcaaggtc gccgatgtgg ttctg
//
LOCUS       DASWQN010000648         3614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2173043, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000648 DASWQN010000000
VERSION     DASWQN010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2173043"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..769)
                     /gene="moaC"
                     /locus_tag="VGC82_11055"
     CDS             complement(293..769)
                     /gene="moaC"
                     /locus_tag="VGC82_11055"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY0423850.1"
                     /translation="MTAFTHFDQAGNAAMVDVSGKAVTARTATARARVVMEAATLAMI
                     QQGSAKKGDVLGVARLAGIMGAKRTADLIPLCHPLPITSVKVDLEADAAANAVEILAT
                     VRTTGQTGVEMEALTAASVAALTVYDMCKAVDRSMRIEGLRVTHKAGGKSGDFTQD"
     gene            complement(766..1572)
                     /gene="trpC"
                     /locus_tag="VGC82_11060"
     CDS             complement(766..1572)
                     /gene="trpC"
                     /locus_tag="VGC82_11060"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY0423851.1"
                     /translation="MPDVLARIAFDVRADVAIRKAIRGIEALRHDIAVRHDATRGFGY
                     ALKQASGAGRYGLIAEIKKASPSGGLIRENFDPAALARAYEAGGATCLSVLTERVHFQ
                     GDPQHLKAARAAVPLPVLRKDFILDPWQVYESRAMGADCILLIMAALTDAQAREMEQV
                     AISLDLDVLVEVHDREELDRALLLETSLIGINNRNLKTLKIDLAVTEELAPLVPPDRF
                     LIAESGIRNTDDLRRLSSVGPNCYLVGESLMRQSDVAAAVRALLGDGIGS"
     gene            complement(1610..2632)
                     /gene="trpD"
                     /locus_tag="VGC82_11065"
     CDS             complement(1610..2632)
                     /gene="trpD"
                     /locus_tag="VGC82_11065"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY0423852.1"
                     /translation="MSANLKQTIARLAAGETLADDQSEAAFDIIMSGEATPAQIGALL
                     MAMRIRGETVAEITGAVRAMRARMTSIEVPAGAIDVCGTGGDGAGTLNVSSTVTFVVA
                     GCGVPVAKHGNRALSSRTGGADVLTALGVNVDAPMASLPAILAEAGCVFLFAPRHHPS
                     MRHAAGPRVELGTRTIFNLLGPLANPARVRRQLTGVFSPDWTRPMAETLAALGHEAAW
                     IVHGMGLDELTIAGQNQVTALSGGSIASFVVEPEDAGLQRAPIEAIRGGDAAFNAAAL
                     LAVLQGAPGAYRDTVLLNAAAALIVAGQAGDLREGVSLAADSIASGAAFAALEALRRV
                     SAVSHA"
     gene            complement(2665..3276)
                     /locus_tag="VGC82_11070"
     CDS             complement(2665..3276)
                     /locus_tag="VGC82_11070"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY0423853.1"
                     /translation="MILLIDNYDSFTFNLYHFLGDIGARSEVWRNDKLSVADALAMQP
                     EAIVLSPGPCTPTEAGICLDLIAAAAGKIPLFGVCLGHQAIGQAFGGRVIRAAEPMHG
                     KVSQITHTGTDILEGLPSPFKATRYHSLVVERDTIPDTLVPTAWTDDGLIMAMHHRTL
                     PIYGVQFHPESIASEHGHTILANFLALARDTNSRGTNSPARAA"
BASE COUNT          628 a         1217 c         1131 g          638 t
ORIGIN      
        1 gcatgattcc cgccggtgag atttggaaag cgttacacca tcggcgatag aaggaggtag
       61 ggacttcgcc tcgtacgtta ttcaaacacc ggatttgcca gtcacggtcg agcttaacag
      121 aattgtagca acttgcggca cgtgctgact ggtcgcctcg tatcaggtcg cgataacctc
      181 gactccccgg caactccagt ttgcgtccat gggggttgga cggcgtttgg agccgtatgt
      241 cggcgccagt ccctcgacca agctgatagt ggggtcgtct cgaagcagac cctcagtcct
      301 gtgtgaaatc tccggatttc ccgccagcct tgtgcgttac ccgcagacct tcgatccgca
      361 tcgaccggtc caccgccttg cacatgtcat agacggtcag tgccgccacg cttgccgctg
      421 tcagcgcctc catttccact cccgtctgcc cggtcgtgcg gactgtcgcg aggatttcca
      481 cggcgttggc ggcagcgtca gcttccagat cgaccttcac ggatgtgatc ggcagcggat
      541 gacacagcgg gatcaggtcc gccgtgcgct tcgcacccat gatcccggcc aagcgagcca
      601 ctcccaggac atcacctttc ttggcggagc cttgctggat catcgccagc gtcgccgctt
      661 ccatcacgac ccgggctcgc gcggtcgccg tccgcgcggt taccgccttg cccgagacat
      721 cgaccatcgc cgcgttcccg gcctggtcga aatgggtaaa cgcggtcacg agccgatgcc
      781 gtcgccaagc agcgccctga cggcggctgc gacatcgctc tgccgcatca ggctctcgcc
      841 gaccaggtag caattcggtc ccacggatga cagccggcgc aggtcgtcgg tgttccgaat
      901 gccgctttcc gcgatcagga accgatccgg aggcaccaag ggcgccagtt cctcggtcac
      961 cgccagatcg atcttcaatg tcttcaggtt acggttattg atcccgatca aagatgtctc
     1021 cagcagcaac gcccgatcga gctcctcccg atcatggact tcgaccagca catcgagatc
     1081 caggcttatc gcgacctgct ccatttcgcg cgcctgcgcg tcggtcagcg cggccatgat
     1141 caggaggatg caatccgcac ccatggcccg gctttcgtaa acctgccacg ggtcgaggat
     1201 gaagtccttc cgcagcaccg gtaacggcac ggcagcccga gcggctttga gatgttgcgg
     1261 gtcaccctga aaatgcacgc gttccgtcag caccgaaagg catgtcgctc cgccggcttc
     1321 gtaggcccga gccagtgcgg ccggatcgaa attctcccgg atcagcccac cggagggcga
     1381 cgccttcttg atttccgcga tcaatccgta gcggcccgcc cccgaggcct gcttcaacgc
     1441 atagccaaag ccccgcgtgg cgtcgtggcg cacggcaata tcatgccgca gcgcctcgat
     1501 cccacggata gccttgcgta tcgccacatc cgctcgcacg tcaaaggcga tgcgggccag
     1561 cacatccggc atcgcctcgt tttcgtccgc cattggctaa cctcattggc tatgcgtggg
     1621 acacggcgct aacccgccgc agggcttcca acgcggcgaa cgcggctccg gatgcgatgg
     1681 aatccgctgc cagggacaca ccttcgcgca aatcgccggc ctgcccggcc acgatcagcg
     1741 ccgcggcggc gttcagcagc acggtatcgc ggtaggcacc gggcgcgccc tgaagcaccg
     1801 ccagcagggc agcggcattg aaggcggcat cgccgccccg gattgcctcg atcggcgcac
     1861 gctgaagacc ggcatcctcg ggttccacga cgaaactggc gattgaacca ccgctcagtg
     1921 cggtcacctg gttctgcccg gcgatcgtca gttcatcgag ccccattcca tgtacgatcc
     1981 aggcggcttc atgccccagc gcggccagcg tctccgccat cgggcgggtc cagtcgggcg
     2041 agaacacccc ggtcaactgc cgccgcaccc gagccggatt ggccagcggc cccagcaggt
     2101 tgaaaatagt acgggtacca agttcgaccc gagggccggc ggcatgacgc atcgacggat
     2161 gatgccgtgg ggcgaacagg aagacgcaac cggcctcagc caggatcgcc ggcagcgacg
     2221 ccatcggcgc atccacattg accccgagcg cggtcagaac atccgccccg ccggtgcgcg
     2281 acgacagcgc ccggttgccg tgcttggcca ccggaacgcc gcagcctgcc accacgaacg
     2341 tcacggtgga ggacacattc agcgttcccg ccccatcgcc tcccgtgccg cagacgtcga
     2401 tcgcgccagc gggtacctcg atggacgtca ttcgcgcccg catcgcgcgc acggcgccgg
     2461 taatctcagc gacggtctcg ccgcggatgc gcatcgccat cagcagcgcg ccgatttgcg
     2521 ccggcgtggc ctcccccgac atgatgatgt cgaaggcggc ctcggactgg tcgtccgcca
     2581 gggtttcacc ggcggccagc cgagcgatcg tctgtttcag gttggcagac attgatcaat
     2641 tcacagccgg ggccgggctc atgctcaggc cgcgcgcgcc ggcgaattgg tgccgcggga
     2701 attcgtatcg cgagccaggg ccaggaagtt ggcgaggatg gtatggccgt gctcgctggc
     2761 gatgctttcg gggtgaaact gcacgccata gatgggcagt gtgcggtgat gcatcgccat
     2821 gatcaggccg tcatcggtcc aggcggtcgg aaccagcgtg tcggggatgg tgtcccgctc
     2881 caccaccagg ctgtgatagc gcgtggcttt aaacggggag ggaaggccct ccagaatgtc
     2941 cgtgccggta tgggtgatct ggctcacctt tccatgcatc ggttcggctg cgcggatcac
     3001 cctgccgccg aatgcctggc cgatcgcctg gtgcccgagg cacacgccga acagcgggat
     3061 cttgccggcc gcggcggcga taaggtcgag gcagatgccg gcctcggtcg gggtgcatgg
     3121 gccgggcgac agcacgatcg cctccggctg catcgccagg gcgtccgcga cagatagctt
     3181 gtcgttgcgc cacacctctg acctggcgcc gatgtcgccc aggaagtggt agaggttgaa
     3241 ggtgaagctg tcgtagttgt cgatgagtag gatcatggcc cgggactttt gtggcaccgt
     3301 atgtttggtc cggcggcgcg cgccggtcaa tcaggaagcg acgtagcgcg tcaggtctgg
     3361 cgccatcgag gcgatccctg gcgtccgctt aatggggtgg ccctgtgcca gctaccccga
     3421 actttcgtgc gtgcggacga agccgcttgt agccgcgcca agcatggcca cggcaccgtt
     3481 ggacgccttc gaccatggcc ggaccgggac tacccatggt tcggtgtgat cggcacgcaa
     3541 ttcgtccgtc cggacgcgac gcaatcctgc accgcggcgg cgtagcgcaa ctgctgcatc
     3601 acgaacagca cgcc
//
LOCUS       DASWQN010000649         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2173201, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000649 DASWQN010000000
VERSION     DASWQN010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2173201"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VGC82_11075"
     CDS             complement(<1..257)
                     /locus_tag="VGC82_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEY0423854.1"
                     /translation="MTDLTASIAELTRDLVVLDSRSSRSNLPVADRIVPELAGFEIER
                     IDYQDAAGVAKRVLVAHRGGPGGIAFSGHMDTVPDTGWQDDP"
     gene            343..1476
                     /locus_tag="VGC82_11080"
     CDS             343..1476
                     /locus_tag="VGC82_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY0423855.1"
                     /translation="MHADYIDPRSGASFPLDQPRWCGDGHAPLLLTPLPGLTRAQIDT
                     TTRSLWRYRAAFPFQIDDPITLGEGMTPLIPRSLAGVQVLLKCEWFNPTCSFKDRGAS
                     VMLSLLRSQGIGHVLEDSSGNGGAAVSAYAAAGGMAATIMAPASTSPAKTVQMRAHGA
                     AVELIPGSRQDTADAAVARSASIFYASHNWHPFFLQGTKTLAYELWEDLGFRVPDNII
                     TPCGAGSNVLGCEIGFSELLRAGQIETLPRIFAAQPANCAPIAAAFMAGSDEPVPTTI
                     SPTMAEGTAIARPIRLREVLGTLRETRGGAVTLTEAEIAKATLDLARIGIYVEPTCAQ
                     AAAAFAKLLETGTVTPEQTTVLVMTGTGLKATPRIAELLGIAL"
     gene            1473..>2363
                     /locus_tag="VGC82_11085"
     CDS             1473..>2363
                     /locus_tag="VGC82_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEY0423856.1"
                     /translation="MKPPVTTPPRVALLGFSIECNRFAPVATESDFRTRTLIEGADLL
                     TEARSPAPRMLGELPGFLAAMDAAGPWQPVPSVLAMAEPNGPVEQAFFDRLMRQWGRD
                     LTAAEPLDGVYLVLHGAGLTTSDHDPEGTLLGLVRRSVGPDVPIVATFDLHANVSEAD
                     VTLLNGFVGYRTNPHLDMRERGAEAAGLLRRLLTGTRTFLAWVRLPIVPPTVMQLTGK
                     DAPNRPYGELIDLGQQRMTEAPYAGRVLNVSVMGGFAYADTPFNGLTVVVTATDQDAA
                     NSLANEIAVAGWSRREQFRPT"
BASE COUNT          390 a          796 c          778 g          399 t
ORIGIN      
        1 gggtcgtctt gccagccggt gtcgggcacc gtgtccatat ggccggagaa tgcgatgccc
       61 ccagggccac cgcgatgcgc caccagcacg cgtttcgcca cgccggcggc atcctggtag
      121 tcgatgcgct cgatctcaaa acccgccagt tccggcacaa tgcggtcggc gactggaagg
      181 ttggaacggg aactccgact gtccaaaacc acgagatcgc gggtcagttc ggcaatagaa
      241 gctgtcaggt cggtcatgcg gctcgatttt taggaccgtt gaagcgtctg tcgcaagacc
      301 acaggcttga gcgcacgctc ggttccgacc acactccacc ccatgcatgc cgattatatc
      361 gatcccaggt ccggtgcatc gttccccctc gatcagccgc gatggtgcgg cgatggccat
      421 gcgccattgt tgctgacgcc tttgcccggg ctgacgcggg cgcagatcga caccacgacg
      481 cgcagtctgt ggcggtaccg cgcggcgttt ccgttccaga tcgacgatcc catcaccctg
      541 ggcgagggca tgaccccgct gatcccacgc tcgctcgccg gggtccaggt gctgctgaaa
      601 tgcgagtggt tcaacccgac ctgcagcttc aaggaccggg gtgccagcgt catgctgtcg
      661 ctgctgcggt cgcaggggat tggccatgtg ctggaggaca gttccggcaa tggcggcgcc
      721 gcggtctcgg cctatgccgc ggcgggcggc atggcggcga cgatcatggc gcccgcctcc
      781 accagcccgg cgaagacggt gcagatgcgg gcgcacggcg cggcggtgga actgatcccg
      841 ggttcgcgcc aggataccgc cgatgctgcc gtggcccggt cggcttcgat attctatgcc
      901 agccacaact ggcacccgtt cttcctgcag gggaccaaga ccctggccta cgaattgtgg
      961 gaggatcttg gtttccgggt gccggacaac atcatcacgc cctgcggggc gggctccaac
     1021 gtgctgggct gcgagatcgg cttttccgaa ttgctgcggg ccgggcagat cgagacattg
     1081 ccgcgcatct tcgccgccca gccggccaat tgcgcaccca tcgccgctgc tttcatggca
     1141 gggtcggatg agcccgtgcc gaccaccatt tccccgacca tggccgaggg taccgcgatt
     1201 gcccgcccga tccggctgcg ggaggtcctg ggcacattgc gcgagaccag gggcggtgcg
     1261 gtgacgctaa ccgaggcgga gatcgccaag gcgacgctcg acctggcgcg catcggaatc
     1321 tacgtggagc cgacctgcgc ccaagcggca gcggcgttcg caaagctgct ggagacaggg
     1381 acggttacgc cggaacagac gacagtgctg gtgatgacgg ggaccgggct gaaggcgacg
     1441 ccgcggatcg ccgaactgct ggggatcgcg ttatgaaacc gcccgtcacg accccgccac
     1501 gggtcgccct cctgggcttc tccatcgaat gcaaccgctt cgcccctgtt gccaccgaat
     1561 ccgatttccg gaccaggacc ctgatcgaag gtgccgacct gctcacggaa gcccgatccc
     1621 ccgcacctcg tatgctcggg gaactgcccg gcttcctcgc ggccatggac gcagccggcc
     1681 catggcagcc ggtaccaagc gtgctggcga tggccgagcc gaacgggccg gtggagcagg
     1741 cattcttcga ccggctgatg cggcaatggg gaagggactt gaccgccgcc gaaccgctgg
     1801 acggcgtgta tctggtgctg cacggcgctg gcctgaccac cagcgaccat gatccggagg
     1861 gcactctgct cggtcttgtc cgacgcagcg tggggccgga cgtgcctatc gtcgccacct
     1921 tcgatctgca tgcgaacgtg tccgaggccg acgtcaccct gctgaacggc ttcgttggct
     1981 accgcaccaa cccacatctg gatatgcgcg aacgcggcgc cgaggccgcc ggcctgctgc
     2041 gtcgcctgct gaccggcact cgcacgttcc tggcctgggt acgcttgccg atcgtgccgc
     2101 cgacggtgat gcagcttacc ggcaaggacg ctccgaaccg gccgtatggc gaactgatcg
     2161 acctcggcca gcagcgcatg accgaagcgc cctatgccgg gcgggtgctg aacgtctcgg
     2221 tcatgggcgg tttcgcctac gccgatacgc cgttcaacgg gctgaccgtg gtggtcaccg
     2281 cgacggatca ggatgccgcg aactcgctgg cgaacgagat cgccgtcgct ggctggtccc
     2341 gccgcgaaca attccgaccc acc
//
LOCUS       DASWQN010000650         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1513002, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000650 DASWQN010000000
VERSION     DASWQN010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513002"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VGC82_11090"
     CDS             complement(<1..239)
                     /locus_tag="VGC82_11090"
                     /inference="COORDINATES: protein motif:HMM:NF022607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HEY0423857.1"
                     /translation="MRAKWPLLAMVLGVGVTYAAYPYVTLYRLGSAIQSADAGTLEAM
                     VNWPAVREGIKEDVCDLALDDPGPKTGDELPPFGAS"
     gene            complement(347..1486)
                     /locus_tag="VGC82_11095"
     CDS             complement(347..1486)
                     /locus_tag="VGC82_11095"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HEY0423858.1"
                     /translation="MLVNPRHGVPGVTILPGGGFRRAKAAITTWPGYAATPLRDLPAI
                     AAEAGIGTLRLKDESSRFGLGSFKALGGAYAVANLLAAEVSRAGLARSANSEDLATGR
                     FAAHTGGITVSCATDGNHGRSVAWGAGRFHCRCVIFVHETVSQARVDAITRFGAAVVR
                     VAGNYDDAVREADRQAATNGWFIVSDTSYPGYTEVPRDVMQGYRLMADEAADQWAGAP
                     PTHVFMQAGVGGAAAAVSVQMRARFTPAPLLVVVEPERAACLLQSARTGTASVVHGDL
                     ATIMAGLACGEPSLLAWQELERAAWAFMSIPDVAAARSLRRLADGGIVIGESGVAGMA
                     GLLASVSDAAAKKDLRLTTDSRVLLFGTEGITDPATYHNTLSLTV"
     gene            1594..2568
                     /gene="denD"
                     /locus_tag="VGC82_11100"
     CDS             1594..2568
                     /gene="denD"
                     /locus_tag="VGC82_11100"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEY0423859.1"
                     /translation="MRISILGGGGFLGRKIAAKLAKDGTLGGKPVTALTLFDMSEPPK
                     PGVAPFPITSVGGDVVTLPDDAIPEGTDVVFHLAAVVSAQAEADYELGRRVNIRGTDA
                     VIDRCRALGTRPRVVFTSSVASFSADQNSTLSDNTRQIPGNSYGAQKAAAELILADAS
                     RRGFLDAVTLRLPTVIVRPGRPNKAASSFFSAIVREPLLGLDTELPVPDDFAVWVCSP
                     RRAVDWLLHAGSMDTSTMGLDRSIDPPGISTTVAHLLQALDEVAPGAASHVKRVEDKA
                     IAAIVSTWPPAFEALHARTLGFSAHEPILDVIHAFIEDDLAATKAERA"
BASE COUNT          493 a          885 c          824 g          497 t
ORIGIN      
        1 gaggcgccga atggcggcag ttcgtcccct gtcttcggac ccgggtcgtc cagcgccagg
       61 tcgcagacgt cctctttgat cccctcgcgc accgctggcc agttgaccat ggcctccagg
      121 gttccggcat cggccgactg tatggccgac ccgagccgat acagggtaac ataaggatat
      181 gcggcgtagg tgacaccaac gccaagcacc atcgcaagaa gtggccattt ggctcgcatt
      241 cgatcgctcc gatgctgcaa tttccggaat agtaacgccc gctggtctga catctttccg
      301 tcaacccttc gataccctag gccacaatta gttgatcgga agttaactaa accgtgagcg
      361 agagagtatt atggtaggtc gccggatccg tgatcccttc agtgccgaac agcagtacgc
      421 ggctgtcggt agttaggcga agatcttttt tggccgcggc atctgatact gaggccagta
      481 atccggccat gcccgccaca ccagactcgc cgatgacgat cccaccatcc gccaatcgcc
      541 gcaagctgcg ggctgccgct acgtctggaa tggacatgaa cgcccaggcc gcccgctcca
      601 gctcttgcca tgccaacagg cttggctcgc cgcacgccaa gccagccatg atcgtcgcca
      661 ggtcgccgtg taccacggat gcggttcccg ttctcgcgct ctgcagcaga cacgccgccc
      721 gctccggttc caccacgacc agcaaaggag cgggggtgaa tcgcgcgcgc atctgcacgg
      781 agaccgccgc cgccgcacca ccgacaccgg cttgcatgaa aacatgcgtc ggcggggctc
      841 cggcccattg gtccgccgct tcatccgcca tcaggcgata gccttgcatc acgtcgcgtg
      901 gaacctccgt atagcccgga tacgaggtat cagagacaat aaaccatcca tttgtcgcgg
      961 cctgacgatc tgcttcccgc accgcgtcgt catagttccc cgcaacccgc accacagcgg
     1021 cgccgaaccg ggtgatcgca tcgacccggg cttggctgac ggtttcgtgc acgaaaatca
     1081 cgcagcggca gtggaagcgt ccggcgcccc atgcgaccga gcggccatgg ttgccgtccg
     1141 tggcgcagga tacggtgatt ccgccagtgt gagcggcaaa gcgaccggtc gccaaatcct
     1201 ccgaattcgc cgaccgcgcc aaacctgcac gcgacacttc cgccgcgagc agattggcca
     1261 ccgcgtaggc accaccgagc gccttgaagc tgccaaggcc aaatcgggag ctctcgtcct
     1321 tgagcctcaa agtgcctatg ccggcctccg cggcgattgc gggaaggtcc cgtagtggtg
     1381 tcgcggcata gccgggccag gtggtgatcg ccgctttcgc gcgacggaaa ccaccgcccg
     1441 gcaagatggt cacaccaggg acgccatgcc gtgggttgac cagcaactcg aatgatcgtg
     1501 gcgcgctcat gcctcaacgg tggaccgctg gctgcgccgg tgcaatctat ccgcggctgg
     1561 cgggccatgc tcggctggcg ggaggacttc acgatgagaa tttcgatact gggtggcggc
     1621 ggtttccttg gccgcaagat cgccgcaaaa ttggccaagg acggcacgct tggtggcaag
     1681 ccggtcaccg cgctgacctt gttcgacatg tccgaacccc cgaagcccgg ggtggcaccg
     1741 ttcccgatta cctcggtcgg cggcgatgtc gtcaccctgc ctgacgacgc gataccagag
     1801 ggcaccgacg tcgtgtttca tcttgccgcg gtggtcagtg cccaggcgga ggcggactac
     1861 gagctgggcc ggcgggttaa tatccgcggc accgacgcgg tgatcgacag atgccgagca
     1921 cttggaaccc gcccgcgcgt ggtgttcacc agttccgtgg ccagttttag tgccgaccag
     1981 aacagcacgc tgtcggacaa tacgcgccag attcccggca acagctatgg cgcacagaaa
     2041 gccgcggccg agctgatcct ggcggacgca tcccgccgtg gcttcctgga tgccgtgacg
     2101 ttgcgcctgc cgaccgtcat cgttcgcccg ggccgtccca acaaggctgc cagttccttt
     2161 ttctccgcca tcgtgcggga gcctttgttg ggcctggata cggaattgcc ggttcccgac
     2221 gatttcgccg tctgggtttg cagtccgcgc cgggcggtgg actggctgct gcatgcgggg
     2281 tcgatggata ccagcacgat gggattggac cgcagcatcg atccgccggg aatcagcacc
     2341 accgtggccc acttgctgca ggcattggat gaggtcgcgc cgggcgctgc atcgcatgtc
     2401 aagcgggtcg aggacaaagc catcgccgcc attgtttcga cctggcctcc tgccttcgag
     2461 gcgttgcacg cccgcacgct gggcttttca gcgcacgagc cgatcctgga tgttatccac
     2521 gcctttatcg aagatgatct ggcggcaacg aaggcggaaa gggcctgacg gagggatccg
     2581 gcggcacttg gcgaccgtcg cacaagcgcg tcagctcatg tttttaccac agtgatgccc
     2641 taatcggagg atcacttatt gcgtgggacc gggtccggtt ccaaaacact tcctgtctt
//
LOCUS       DASWQN010000651         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_62613, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000651 DASWQN010000000
VERSION     DASWQN010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62613"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VGC82_11105"
     CDS             <1..593
                     /locus_tag="VGC82_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase, family 2"
                     /protein_id="HEY0423860.1"
                     /translation="LLLNNDVDVINPGWLREMVSHAIRPGIGAVGAKLLYPDGTIQHG
                     GVTVGVGGVAGHQYLHHPRGDVGYFGQLVLARNITAVTGACLLLRRQSFQEVGGLDEI
                     SLPVAFNDIDLCLKLVARGYRNLWTPYAELYHHESASRGSDLAGKKAARFMAEVSVMR
                     QRWGEVLDHDPYWNPNLALHVTEVSLAFPPRDPVIT"
     gene            complement(594..2360)
                     /locus_tag="VGC82_11110"
     CDS             complement(594..2360)
                     /locus_tag="VGC82_11110"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003618295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY0423861.1"
                     /translation="MSAPALSALRQELTRQGIDGFIVPRADEHLGEYVPAAAERLAWL
                     TGFTGSAGVAVVLDATAAVFTDGRYVLQLAEQTDPGLWQRLHLTEDPPPSWLVRHAPK
                     NARIGYDPLLISDEGLARYTETGLELVPLEHNPIDRVWTDRPPAPCTPAEPHPLAYAG
                     RSSEDKRADVAKMLREAAQDAAVLTDPASIAWLLNIRGGDVPFTPFALGFALIHADGA
                     TELFIDPRKVPEQTRSWLGNAVSVADRDALLPALQSLKGKTVRVDQTGSPVWFAQQLR
                     KAGAAVAFGSDPCLLPKACKNEGEQSGARAAHLRDATAVCRFLHWLSITAPAGRETEM
                     SAANMLLGFRRQVEGFRGESFPAISGAGEHGAIIHYRVTEDSNRSIKPNEVYLIDSGA
                     QYPDGTTDITRTVWTGPGDPPDSVRDRVTRVMQGHIALATAMFPKGVGGAHLDALARQ
                     ALWQVGLDYDHGTGHGVGSFLSVHEGPVSISRLARPIPIAAGMILSNEPGYYLPDHYG
                     IRLENLLLVQPASLPDAVKPFLCFETLTLAPFDRRLLKPALMTARDIAWLDAYHARVL
                     RIVGPTLEGAEQEWLTAACAPL"
     gene            2550..>2742
                     /locus_tag="VGC82_11115"
     CDS             2550..>2742
                     /locus_tag="VGC82_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423862.1"
                     /translation="MSDRTADSQFSFHLPSLTYIDAKWEEPNLRTPTAPVQPSLTAKL
                     SGWLVRRLQAIVAWRRDQQA"
BASE COUNT          520 a          872 c          838 g          512 t
ORIGIN      
        1 tcctgctgct gaacaacgat gtggatgtga tcaatccggg ctggctgcgg gaaatggtgt
       61 cgcacgcgat tcgtccgggc atcggagcag tcggcgccaa attgctctat cccgacggca
      121 cgatccaaca tggcggtgtg accgtcgggg ttggcggggt cgcaggccat cagtatctac
      181 atcatccccg cggcgacgtg ggctacttcg gccagctcgt gctggcgcgg aacatcaccg
      241 cggtgacggg agcctgcctg ctgcttcgcc gccagtcgtt tcaagaggtt ggcggccttg
      301 atgaaatttc gctgccggtg gcgttcaacg acatcgacct gtgcctcaag ctggtcgcgc
      361 gaggctaccg caacctttgg accccgtatg ccgagcttta ccaccacgag tcggcatcgc
      421 gcgggtctga tctggcaggg aagaaggcgg cgcggttcat ggctgaggtt tccgttatgc
      481 gccagcggtg gggtgaagtg ctggaccatg atccgtactg gaatccgaac ctggcgctgc
      541 acgtgaccga agtctcgctg gcgttcccgc cgcgtgatcc ggtgatcacc tgatcaaagc
      601 ggcgcacagg ccgctgtcag ccattcctgc tccgcccctt ccaaagtcgg acccacaatt
      661 cgcaggacgc gagcatgata cgcatcgagc catgcgatat cgcgagccgt catcagcgct
      721 ggcttcagca gccgccggtc gaagggtgcg agggtaaggg tttcaaagca caagaagggc
      781 ttgacggcat ccggcaggga agcgggttgc accagcaaca gattctccag cctgatgccg
      841 tagtggtccg gcaggtagta acccggttca tttgacaaaa tcatacccgc cgcgattggg
      901 atcggacgtg ccagccgcga gatactgacc ggtccctcgt gcaccgacag gaaactgccc
      961 acgccatgtc ctgtgccatg gtcgtaatcg aggccgacct gccaaagcgc ctgacgcgcc
     1021 aacgcatcga ggtgcgcccc gcccacaccc ttcggaaaca tcgcggtggc aagcgcgatg
     1081 tgcccttgca tcacccgggt tacccgatcc cggacactgt ccggcgggtc gccaggaccg
     1141 gtccagaccg tgcgcgtgat gtccgttgtc ccgtccggat actgcgcacc ggaatcgatc
     1201 agatacactt cgttcggctt gatcgaccgg ttagaatcct ccgtcacccg gtaatgtatg
     1261 attgcgccgt gttccccagc accagagatt gcagggaagc tctcgccgcg gaacccttcg
     1321 acctggcggc ggaagcccag cagcatgttg gccgcggaca tctcggtttc ccgtccggct
     1381 ggtgccgtga tggaaagcca atgcaggaag cggcatacgg cagtcgcatc gcgcagatgt
     1441 gccgcgcggg caccggactg ctcgccctcg ttcttgcacg ccttgggcag caggcaggga
     1501 tcgctgccga atgcaaccgc tgcccccgcc ttacggagtt gctgggcgaa ccagacgggc
     1561 gatccggtct gatcgacccg cacggtcttt cccttcaagg attgcagcgc cggcagcagc
     1621 gcatcccgat ccgcgaccga cactgcgttg cccaaccacg agcgggtctg ttccggcacc
     1681 ttccgaggat cgatgaacag ttcggtcgca ccatcggcat gtattagggc gaaccccaag
     1741 gcgaatggcg tgaacggcac gtcgccacca cggatgttca gcagccaggc gatcgacgct
     1801 ggatcggtga gcacggctgc gtcctgagcg gcctcccgca gcatcttcgc cacgtcagct
     1861 cgtttatcct ccgacgagcg gccagcgtag gccaaaggat gcggttcggc cggagtgcag
     1921 ggggcagggg ggcggtccgt ccacacccga tcgatgggat tatgctccag gggcaccagt
     1981 tcgaggccgg tttcagtgta gcgggcgagg ccttcgtcgc tgatcagcag tggatcgtag
     2041 ccaatgcggg cgttcttggg cgcatgtcga acgagccagg acggcggcgg atcctccgtc
     2101 aggtgcagac gctgccacaa ccccggatcg gtctgttccg cgagttgcag gacgtatcgg
     2161 ccatctgtga acacagccgc cgtcgcgtcc aggacaaccg ccacgcccgc actgccggta
     2221 aaccctgtca gccacgccag acgctcagcc gcggctggaa catattcgcc gagatgttcg
     2281 tccgcacgcg gaacgataaa gccgtcgatg ccctgacgcg tcaattcctg ccgcaatgcg
     2341 gataacgccg gagcgctcat tttcgacccc ttgcatccat aaacgaaaaa ttcatagcta
     2401 tgccgaagcc atgcactgga agcatggcag ttttgggctt ttcactcttt atcgaagcgt
     2461 aaccgatcct atgttgcact gcatcatacc tgggggcgcc cgccaccccg acgtcgcgta
     2521 gcacccgggt ttgtgtatgg acactagcta tgagcgacag aaccgctgac tcccaattct
     2581 cattccatct tcccagcttg acctatatcg acgcaaaatg ggaagagccg aacctcagaa
     2641 cacccaccgc gccggtacaa ccgtcactta cggccaagtt gtctggctgg ttggtccgtc
     2701 ggctccaggc gatcgtggct tggcgccgtg atcagcaggc tg
//
LOCUS       DASWQN010000652         3379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1513155, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000652 DASWQN010000000
VERSION     DASWQN010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513155"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VGC82_11120"
     CDS             complement(<1..401)
                     /locus_tag="VGC82_11120"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY0423863.1"
                     /translation="MQDFITRIPKAELHVHLEGCIEPEMMFDLARRNGVTLPWSSPRA
                     LLAAYEFADLRSFLDLYFQGCLVLVRQQDFYDVTMAYLRRARADGVVRAEVFIGPQSF
                     TERGIALATVLDGVLAAMRDAADEFGISAGL"
     gene            544..1719
                     /locus_tag="VGC82_11125"
     CDS             544..1719
                     /locus_tag="VGC82_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY0423864.1"
                     /translation="MRPMEGMRVIALEHAVAAPLCTRHLADLGADVIKIERPGDGDFA
                     RAYDDYVNGICSHFIWLNRGKRSVTLDVKNPDARAALNTLIEGADVLVQNLAPGAAAR
                     LGLTHEALKPANPGLVVCDISGYGESGPFVQKKAYDLLIQAESGLISVTGSANEPSRV
                     GISIADIATGMYALTGILGALLRRGRTGQGANVKVAMLDALGEWMTYPMLRHAYAGSA
                     PPRVPTSHPAIAPYGAHRTMDGQVILGLQNEREWAVFCQKVLGSSVIQTDPRFATQNV
                     RRENRQALTAVIEDFFSDKTSAEVAALLDAANIANGRLNEPIDVWNHVQFSARDKWRT
                     VNTEAGPVQALLPPFTFTDEAAPMGDVPSVGQHTDEVLGEIGFSAERIAAMRAAGAV"
     gene            1716..2216
                     /locus_tag="VGC82_11130"
     CDS             1716..2216
                     /locus_tag="VGC82_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY0423865.1"
                     /translation="MSDFDPTQHRMVPEQRWFDDFRIGERFVLPSRTMTEAVFLAFQG
                     ASGDNHPVHYDVEFCKARGMPGLLAHGLQSLVQTAPGAGLFPYLVEDSLVGFIEQSSR
                     FLKPVFAGDTVYPALEIDELTANRTTGVLGFKSTIHNQRRELVLDGRQRYLLRRRLNT
                     GADLKG"
     gene            complement(2206..2688)
                     /locus_tag="VGC82_11135"
     CDS             complement(2206..2688)
                     /locus_tag="VGC82_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423866.1"
                     /translation="MMRATLFGLLILAGTSIGSTNGYADEAPEPALLERTDAFDKAYG
                     ASFYEFDACGDGIGGRIYRSALVDKLKQCPFSEAARKRFQLRATAQRRKSNQVMAKLI
                     EDNGGLPMRLEGMTRSCREQFDSPEYRLVRSRLDDFSSGKLGSDAVITQPCDAPEITP
                     "
     gene            complement(2685..3299)
                     /locus_tag="VGC82_11140"
     CDS             complement(2685..3299)
                     /locus_tag="VGC82_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0423867.1"
                     /translation="MRLGMLMLAVIVSLPADAQAAADAAAGKDKAQVCAACHGENGVS
                     SMPGVPSLAGQQDQFIQWQLVFFRSGRRPNPAMAPLVADLGDEDVRNLGAYFSGLPYN
                     TRTATEQPDAGLAAKGRTIAAQHRCASCHMDNYRGSRAAAAIANQREDYLIKALADYR
                     SGTRPSVGVAAMTEAAAGLSDDEIAAVAHYVATLAPPPRKQGTR"
BASE COUNT          632 a         1099 c         1047 g          601 t
ORIGIN      
        1 aacaggccgg cgctgatgcc aaattcatcc gccgcgtccc gcatcgcggc cagcacgcca
       61 tccaggaccg tagccagtgc gatcccgcgt tcggtgaagc tttgcggccc aatgaagacc
      121 tcggctcgca ccaccccatc cgcccgggcc cggcgcagat acgccatggt gacgtcataa
      181 aaatcttgct gccgcaccag aaccagacag ccctggaagt agaggtccag gaaagaccgc
      241 aaatcggcga actcataagc tgcaagcagc gcccgtggag aactccaggg cagcgtcaca
      301 ccattccgcc tggcgagatc gaacatcatt tccggctcga tgcatccttc cagatgcaca
      361 tgcagttcgg ctttcggaat cctggttatg aaatcctgca ttggcgataa aactccggtc
      421 atggcccaac ccaccggata catcttcgcc ggcccgccaa ccagtccgcc gtcctgtttt
      481 tgacatcgcc aggaaccagg gctttcctcc tgtgcgaacc cagacatcaa aggaaacgcc
      541 cggatgcgtc ccatggaagg catgcgcgtg atcgcgctgg aacacgccgt cgcggcgcca
      601 ctgtgcaccc gccatctggc cgatctcggc gccgacgtta tcaagatcga gcggcccggc
      661 gacggcgact tcgcccgcgc ctacgacgac tacgtcaacg gcatttgcag ccacttcatc
      721 tggctcaacc ggggcaagcg aagcgtgacg ctggacgtaa aaaacccgga tgcaagggca
      781 gcattgaaca cgctaatcga aggcgccgat gtgctggtac agaaccttgc accgggcgcc
      841 gccgcaagac tcggcctgac gcatgaagcg ctgaagccgg ccaatcccgg gctggtcgtc
      901 tgcgacatct ccggctacgg cgaaagcggc ccgttcgtgc aaaagaaggc ttacgacctg
      961 ctgatccagg ccgaatccgg gctgatcagc gtcaccggat cggcgaacga accctcccgc
     1021 gtcgggatat cgatcgccga catcgcgacc ggaatgtatg cgctgaccgg catcctcggc
     1081 gcgctgctgc ggcgcggccg cacggggcag ggggcgaacg tcaaggtcgc catgctcgat
     1141 gcgctcggcg aatggatgac ctatccgatg ctgcgccatg cctatgccgg gtcggcaccg
     1201 ccgcgcgtgc ccaccagcca ccccgccatt gccccgtatg gcgcgcatcg aacgatggac
     1261 ggccaggtga tcctgggcct gcaaaacgaa cgtgaatggg cggttttctg ccagaaggtg
     1321 cttggcagtt cggtaataca gaccgatcca cgtttcgcta cacagaacgt ccggcgggag
     1381 aaccggcagg cgctgaccgc ggtgatcgag gatttcttca gcgacaagac gtcggcggag
     1441 gtggcggcgc tgctggatgc cgcgaatatc gccaatgggc ggctgaacga gccgatcgat
     1501 gtctggaacc atgttcagtt ctcggctcgt gacaagtggc ggacagtgaa caccgaggcg
     1561 gggccggtcc aggcgttgct gccgccattc acgttcaccg atgaagcggc accgatggga
     1621 gatgtgccgt cggtcggcca gcacactgac gaagtgctcg gggaaatcgg cttctctgcc
     1681 gaacggatcg cggcgatgcg cgcggcgggg gcggtatgag cgatttcgac cccacccagc
     1741 accgcatggt gcccgaacag cgctggttcg acgattttcg cattggtgaa cgcttcgtgc
     1801 tgcccagccg gacgatgacc gaggcggtgt tcctggcctt ccagggcgcc agcggcgaca
     1861 atcatcctgt tcactacgac gtggagttct gcaaggcgcg cggcatgccc ggcctactgg
     1921 cgcatgggct gcagtcgctg gtgcagacgg ctccgggcgc cggattgttc ccatacctcg
     1981 tggaggactc gctggtcggt ttcatcgagc agtccagccg gtttctcaag cccgtcttcg
     2041 ccggcgacac ggtctatccg gcgctggaaa tcgatgagct gacggcgaac cggaccaccg
     2101 gcgttctcgg gttcaagagc acgatccaca accagcgccg ggaattggtg ctggacggcc
     2161 ggcagcgcta tctgttgcgg cgccggctga acacaggggc cgacctcaag gggtaatctc
     2221 cggcgcatcg cagggctggg tgatgacggc gtccgatccc agcttgccgc tggagaaatc
     2281 atcgagccgg ctacgaacca agcgatactc cggactgtcg aactgttcgc ggcagcttct
     2341 cgtcatgcct tccaggcgca tcggcagtcc gccgttgtct tcgatcaact tcgccatgac
     2401 ctggtttgac ttgcgtcgtt gcgccgtcgc ccgaagctgg aagcgcttcc tggcagcctc
     2461 ggaaaaaggg cattgcttca gcttgtccac caatgcgctc ctgtagatac ggccgccgat
     2521 gccgtcgccg caggcgtcga attcgtagaa cgatgcgcca taggctttgt cgaacgcgtc
     2581 ggtgcgctcc aacaacgccg gctctggggc ttcgtcggcg tatccgttcg tgctgccaat
     2641 cgaggttcca gccagtatca gcagaccgaa cagggtggcg cgcatcatcg cgttccttgc
     2701 ttccgcggcg gcggtgcgag cgtcgcgaca taatgcgcca ccgcggcgat ttcgtcatcg
     2761 ctcaggccgg cggctgcctc ggtcatcgcg gcgaccccaa cgctcgggcg ggttccggag
     2821 cggtaatccg ccagcgcctt gatcaggtag tcctcccgct ggttcgcgat cgccgcggcc
     2881 gcccggctgc cacggtagtt gtccatatgg caactcgcac aacggtgctg tgccgcgatg
     2941 gtccggccct ttgccgcgag cccggcatcg ggttgttcgg tcgccgtcct ggtgttgtac
     3001 ggcaggccgg aaaaatacgc cccgaggttg cggacatcct cgtcgccaag gtcggcgacg
     3061 agcggcgcca tggccgggtt cggcctgcga ccggaacgga aaaacacaag ctgccactgg
     3121 atgaactgat cctgttggcc ggcgagcgac ggaacccccg gcatcgagga aactccgttc
     3181 tcgccatgac aggctgcaca aacctgtgct ttgtccttgc ctgcggctgc gtccgcggcg
     3241 gcttgcgcat cggctggcag cgagacgatc actgccagca tcagcatacc tagcctcatc
     3301 aagccccgat tgtcctattt cgcgtagctg atgcgataga tcgctccggc ccagtcatcc
     3361 gaaaccagca atgacccat
//
LOCUS       DASWQN010000653         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_458045, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000653 DASWQN010000000
VERSION     DASWQN010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458045"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..702
                     /locus_tag="VGC82_11145"
     CDS             298..702
                     /locus_tag="VGC82_11145"
                     /inference="COORDINATES: protein motif:HMM:NF039363.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6163 family protein"
                     /protein_id="HEY0423868.1"
                     /translation="MSSERIESEENVWTRRLVLFLRIMALIAVTKGLYHWAQVTGFIG
                     GEEDAFENQPMAWQAATIYFAVIELVAAVGLWLATPWGAVVWLTTVVSMAVIELMFPG
                     IYGGSLLVVAVEALMLAAYLALAWMAARERPP"
     gene            1247..1762
                     /locus_tag="VGC82_11150"
     CDS             1247..1762
                     /locus_tag="VGC82_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY0423869.1"
                     /translation="MNKAVAMAVETAEQAAGQVPVQPLYLEALTLVERLHRRLLDVIK
                     DEFDRRGRADINSVQALLLYNIGDKELTAGELRTRGYYLGSNVSYNLKKLVELGFLDH
                     QRSRVDRRSVRIRLTPQGQEIRRVVDALYQKHVKTVEQVGGISNEEFATLNKSLHRLE
                     RFWTDQILYRL"
     gene            1954..2178
                     /locus_tag="VGC82_11155"
     CDS             1954..2178
                     /locus_tag="VGC82_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423870.1"
                     /translation="MLVERGLRVMNVEVVGDAYAIASNYLRRSGAIPDSFATNERLLQ
                     IVVDLFQQGEFNRIKLGNKAITRYEADALV"
     gene            2227..2448
                     /locus_tag="VGC82_11160"
     CDS             2227..2448
                     /locus_tag="VGC82_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423871.1"
                     /translation="MEAAIDRIMMTYDLLMNRSAAASAEARAKVTEYVTTLSAAGEKD
                     IHRLTVCGLTYLRELDGSSDPVKAGFTGL"
BASE COUNT          493 a          783 c          761 g          521 t
ORIGIN      
        1 gggaacggcg gtcgccggcc gcggcgcgtc gcgcatttcg atcggacgcc cgaatttgat
       61 cgccatgtga tgtctctcct gaaggcggca gtctggttag ctcgtgttcc acaattctaa
      121 cactttgcat ggcgtccgtc accgagcctt gacctgccgc aagcccgatt gattttgccg
      181 cgccgagggg ccatgagttg gccaatgagc atccattcca gcccatgacc agcgatcagc
      241 atgtctgaaa ccccatcgcg cgaccattcg cgggacaacg ccatttcggt ggcggcgatg
      301 tcgtcggaac ggatcgagtc cgaggagaac gtctggacga ggcggctggt gctgttcctg
      361 cggatcatgg ccctcatcgc cgtcaccaag ggcctctatc actgggcgca ggtcacgggc
      421 ttcatcggcg gcgaggaaga tgcgttcgag aaccagccga tggcctggca ggccgccacg
      481 atctatttcg cggtgatcga actggtcgcc gcggtcggcc tgtggctcgc gacgccctgg
      541 ggcgcggtgg tgtggctcac caccgtggtg tcgatggcgg tgatcgaact gatgtttccc
      601 ggcatctatg gcggcagcct gctggtggtg gcggtcgaag ccttgatgct ggccgcctat
      661 ctcgcgctgg cctggatggc ggcgcgcgaa cggccgccat agcggttact tgcgtgctgc
      721 aattcacgcg cgatgaagga ttttgcagtc gggtggcttc atccttcgag acggcgctgt
      781 cgcgcctcct caggatgagg tcctctaacc cctcgtggtg aggagcgcgg cgacgccgcg
      841 cgtctcgaac catgaggccc gtgcccgatg accacaatca tcatcgctga actggctaaa
      901 ccggggttcg cccccctaat aatttcgccg gtcccgcggt aaccatttgg tcaccttaaa
      961 cgtcccggtc atacctgtta cgcaggcctt tccagtttga acgatgctgt tcccatatgc
     1021 gacaggccgc gcggacgaag cgtgaacagg gcctccgcac cgtgaatcaa aagttgcgaa
     1081 aagcccttta tccatgggct taatcggcga ttcaccgtct taaattcatg ctctctttat
     1141 tgtcttattt aagcggatgt tcaaacggcc cccttaagtt gaggctatca ggcgggacaa
     1201 aagtttcgtc gaataagtcg ataaaaacga caacagggga agtgtcatga ataaagccgt
     1261 tgcgatggcg gtggaaaccg ctgaacaggc tgccggccag gtgccggtgc agccgttgta
     1321 tctcgaagcg ttgacgctgg tcgagcggct gcatcgccgc ctgcttgacg tcatcaagga
     1381 tgaattcgat cgccgtggcc gcgccgacat caattcggtg caggcgctgc tgctctacaa
     1441 tatcggcgac aaggagctga ctgcgggcga actgcgcacc cgcggctact atctcggctc
     1501 caacgtgtcc tacaatctga agaagctggt cgagctcggc ttcctcgatc atcagcgttc
     1561 gcgggtcgat cgccgctcgg tgcgcatccg cctgaccccg cagggtcagg agatccgccg
     1621 cgtcgtcgac gcgctgtacc agaagcacgt caagacggtg gagcaggtcg gcggcatctc
     1681 gaacgaggag ttcgcgacgc tcaacaagtc gctgcaccgg ctcgagcggt tctggaccga
     1741 ccagatcctc tatcggctct gagaccttct tcggccaagc cggcgtgttg aaaagaccgg
     1801 cagccaccga aaacaaaaaa gtgacttccc caagcgcatc ggtccggtct tccggcccgg
     1861 tgcgctctgt tttgtcggga taaaacccgt cttgccacaa tggaactttc ctgcgcctgg
     1921 ctgcttatcc gtttgtagcg gaggacctga gtgatgctgg tcgagcgtgg attacgggtg
     1981 atgaatgtgg aggtggtcgg cgatgcctat gccatcgctt cgaactacct gcgccggtcc
     2041 ggcgccattc ccgacagctt cgccaccaac gagcgattgc tccagatcgt cgtcgatctg
     2101 tttcagcaag gcgagttcaa ccgcatcaag ctcggcaaca aggccatcac caggtacgaa
     2161 gcagacgcac tggtctgagc gcaaccgccg ccctgatcgc gactgcccac aagcaaagag
     2221 gatttcatgg aagccgccat cgaccgcatc atgatgacct atgatctcct gatgaaccgg
     2281 tccgccgccg ccagcgccga agcccgcgcc aaggtgaccg agtacgtcac cacgctgtcc
     2341 gccgccgggg aaaaagatat tcatcggctc accgtgtgcg gactgaccta cctgcgcgaa
     2401 ctcgacggct cgagcgatcc ggtcaaggcc ggatttactg ggctgtagtc tctcgcttca
     2461 tccctgatcc tctggaatac tggatcgccc ggtcacaccg ggcgacgacg tattgcgctg
     2521 gaggacccca tgctcacgct gcatcatctc aacgactc
//
LOCUS       DASWQN010000654         2876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2305310, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000654 DASWQN010000000
VERSION     DASWQN010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2305310"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..2291
                     /locus_tag="VGC82_11165"
     CDS             126..2291
                     /locus_tag="VGC82_11165"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEY0423872.1"
                     /translation="MDYVKSSGIRVAKPLYDFVNSEALPGTLIDSDTFWGGFASLLAD
                     LAPRCKALLAKRDSIQQQLDAWHLANKGKPRDSEQYLSFLRGIGYLVEEPASVAVDPA
                     KVDAEIATLAGPQLVVPVTNARYALNAANARWGSLYDALYGTDAIPEDAGATRGPGYN
                     KVRGAKVVAKAREILDAAVPLDSGYHADTVGYSVRDGALLAALKPGGTTGLKEPSQFV
                     GYLGEASAPSSVLLRHNGLHLEIVIDRSNPIGKDDPAGVADVLVEAALTTIQDCEDSI
                     AAVDAEDKVLAYRNWLGLMKGTLQDTFEKAGQKMTRKLNPDRQYTKPGGGTLWLSGRS
                     LMLIRNVGHHMYTDAVLDADGAEVPEGFLDAAVTSLIGLHDLNSKGPIKNSRAGSIYI
                     VKPKMHGPEEVALTAELFGRVENILGMAANTLKMGIMDEERRTSANLKSCIAAAAHRV
                     VFINTGFLDRTGDEIHTSIEAGPMIRKNEMRGTAWIKAYEDQNVDIGLECGLRGKAQI
                     GKGMWAAPDRMAEMLQQKVGHPMAGANTAWVPSPTAATLHALHYHQVDVGVRQTELAS
                     RPRASLKDLLTIPLAEGQNWSPDDLRQELDNNCQGILGYVVRWIDQGIGCSKVPDIHD
                     VGLMEDRATLRISSQHIANWLHHGVVTQEQVTEALQRMAVVVDRQNAADPLYRPMAPR
                     FDGVAFKAACDLIFQGRSQPNGYTEFILHRRRREAKAAA"
     gene            complement(2366..>2876)
                     /locus_tag="VGC82_11170"
     CDS             complement(2366..>2876)
                     /locus_tag="VGC82_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0423873.1"
                     /translation="NGAMGYSVPAAVGAKIAFPDRMVVSMVGDGGFMMTGQEIATAFH
                     HGVAPIVLVFNNQMYGTIRMHQEKAYPWRVSGTALTNPDFCKYIEAFGGHGEVVTMTE
                     EFAPAFRRAVESGKPALIELRTNPEQITTRTTIADLRAGKQPPKAAAKPKRAAAPVHR
                     VSTSGKKRG"
BASE COUNT          550 a          835 c          957 g          534 t
ORIGIN      
        1 cgatgttcac ccaaaatcca cggcgatatc cccttcgtgg gcgttggcca gggctggcga
       61 ggtcaaccaa atgcgtccaa taatggggat tgcagccgct tttcgtccac cgggagccag
      121 tcttaatgga ttatgtcaaa tcgtctggta ttcgcgtcgc caagcctctt tacgattttg
      181 tcaacagcga ggccttgccg gggaccttga tcgacagtga cacattctgg ggcgggttcg
      241 cctccttgct cgccgatctc gcaccgcgct gcaaagcgct gcttgcgaag cgcgacagta
      301 tccagcagca gctcgacgcc tggcatcttg ccaacaaagg gaaaccacgg gacagcgaac
      361 agtatctgag ctttttgcgc ggaatcggat acctggtcga ggaacccgcc tcggtcgcgg
      421 ttgacccggc caaagtcgat gcggagatcg cgacgctggc cggtccgcag cttgtggtcc
      481 cggtaaccaa cgctcgatat gcgctgaatg ctgcgaacgc tcgctggggc agcctttatg
      541 acgcgctgta tggcacggac gcgattccgg aggacgcagg tgcgacccgt ggacccgggt
      601 acaacaaggt tcggggcgcc aaggtggtcg ccaaggcgcg cgaaatcttg gacgcggcag
      661 tgccgctgga tagcggctat cacgcggata cagtcggcta ttcggttcgg gacggggcac
      721 ttctggccgc cctgaagccc ggtggcacaa ccgggttgaa ggagccctct cagtttgtcg
      781 ggtacctggg ggaggcgtca gcgccgtcgt ccgtactgct ccggcataac gggcttcatc
      841 ttgaaatcgt gatcgaccga tccaatccga tcggcaagga cgaccctgcc ggtgttgccg
      901 atgtgctggt ggaagcggcg ctaaccacga tccaggactg cgaagattcc atcgcggcgg
      961 tggatgccga ggacaaggtc ctggcctatc gcaactggct gggcctgatg aaaggcacgc
     1021 tgcaggatac cttcgagaag gctggccaga agatgacccg caagctcaac cccgaccggc
     1081 agtacaccaa gccgggaggc ggcacgctgt ggctgtccgg ccgaagcctg atgctgatcc
     1141 gcaatgtcgg tcatcacatg tacaccgatg ccgtgctcga tgccgacggt gcggaggttc
     1201 cggaaggatt tctggacgcg gctgtcacgt cgttgatcgg cctgcatgac ctgaacagca
     1261 aggggccgat caagaacagc cgagccggat cgatctatat cgtgaagccg aagatgcacg
     1321 ggcctgagga ggtggcgctg accgctgagc tgttcgggcg cgtggaaaac atcctgggga
     1381 tggccgcgaa cacgctgaag atgggcatca tggatgaaga acggcggacg tcggccaacc
     1441 ttaagtcctg catcgccgcg gcggcgcacc gggtggtgtt catcaacacc gggttcctgg
     1501 atcggacggg ggatgaaatt cacacctcga tcgaggctgg gccgatgatc cgcaagaacg
     1561 agatgcgcgg gacagcctgg atcaaggcgt acgaggacca gaacgtcgat atcggcctgg
     1621 aatgcgggct gcggggcaag gcccagatcg gcaaggggat gtgggccgca ccggatcgca
     1681 tggcggagat gctgcagcag aaggtcgggc acccgatggc gggagcaaac accgcttggg
     1741 tgccgtctcc gaccgccgcg acgttgcatg cgctgcatta ccatcaggtc gatgtcggtg
     1801 ttcggcaaac ggaactggcg tcgcggccgc gggcgtcgct gaaggatttg ctgaccatcc
     1861 cattggcgga gggccagaac tggtcgccgg atgatttgcg gcaggagctc gacaacaact
     1921 gtcagggcat tctcgggtac gttgtgcgct ggatcgacca agggatcggt tgctccaagg
     1981 tgccggacat ccatgacgtg ggactgatgg aggaccgggc gacgttgcgg atttcgtcgc
     2041 agcacatcgc caattggctg caccacgggg tggtgacgca ggagcaggtg accgaggcgt
     2101 tgcaacgcat ggcagttgtc gttgaccgac aaaatgctgc cgatccgctg tacaggccga
     2161 tggcgccgcg gttcgatggc gtggcgttca aggcggcgtg cgatctgatc tttcagggac
     2221 ggtcgcagcc gaacggctat acggagttca tcctgcatcg ccgccggcgg gaggcaaaag
     2281 ccgccgcgta gtcggtggtg acggcgcggc atggtatggc ccttaccggc cgcgtatcgc
     2341 gcacaatttg gcgaattgcg attgcttacc cccgcttctt gccagaggtg gaaacacgat
     2401 gcaccggtgc ggcggctcgc tttggtttcg ccgcggcttt cggcggttgc ttccccgccc
     2461 gcaaatccgc gatcgtggtt cgagtggtga tctgctcggg attggtgcgc aactcgatca
     2521 acgccggctt gccgctttca acggcccggc gaaaggctgg cgcgaattcc tcggtcatgg
     2581 tcaccacttc gccgtggccg ccgaacgcct cgatgtattt gcagaaatcc gggttggtca
     2641 gcgcggtgcc ggaaacccgc cacggatagg ctttctcctg gtgcatgcgg atcgtgccgt
     2701 acatctggtt gttgaacacc aatacgatcg gcgcgacgcc atggtggaaa gccgtcgcga
     2761 tctcctgccc agtcatcatg aacccaccgt cgccgaccat gctgacgacc atgcgatccg
     2821 gaaaggcgat cttggcaccg accgcggccg gcacgctgta gcccatcgcg ccgttg
//
LOCUS       DASWQN010000655         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1645893, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000655 DASWQN010000000
VERSION     DASWQN010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645893"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..1083)
                     /locus_tag="VGC82_11175"
     CDS             complement(358..1083)
                     /locus_tag="VGC82_11175"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HEY0423874.1"
                     /translation="MGKDRSGAGAEGGLRAVKPPPDRLYGRARGHKLRPRQEVLLDVT
                     LPRLALPDPLPPFPGPLWLEVGFGGGEHALAQVRAHPDVTLIACEVFENGICSLLSAL
                     VPEGEEATAPLPPNLRLWTDDARILLRALPDGCLDQLFLMFPDPWPKARHAKRRFVHP
                     AMLPLLARVMKFGAEWRVASDDPTYQAWVRDVMASQTLFDTVAIHTGRPDGWPPTRYE
                     AKALRAGRQPLYWVFQRRRERVG"
     gene            complement(1032..2219)
                     /gene="metK"
                     /locus_tag="VGC82_11180"
     CDS             complement(1032..2219)
                     /gene="metK"
                     /locus_tag="VGC82_11180"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY0423875.1"
                     /translation="MRDNGEYLFTSESVSEGHPDKVADRISDTVLDAFLTADPYARVA
                     CETLVTTNRVVLAGETRGPATVTPELLTHLTRLAIRDIGYEQEGFHWANADVAVYLHS
                     QSSDIAQGVDSAGNKDEGAGDQGIMFGYACRETETLMPAPLYYAHEILRTIRDLRKAG
                     DRSVAGLLPDAKSQVTLRYVDGKPAGATSVVVSTQHMAGLEQPEIKRLLWPLVETILP
                     KGWMCTEEEFHVNPTGNFVIGGPDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKV
                     DRSAAYACRYLAKNVVAAGLADRCTLQISYAIGVARPLSVYVDLHGTGRDIDESRLER
                     TLRDVMNLSPRGIREHLKMNRPIYAPTSAYGHFGREPDEAKGTFTWEKTDLVPALRAA
                     FGR"
     gene            complement(2327..>2542)
                     /locus_tag="VGC82_11185"
     CDS             complement(2327..>2542)
                     /locus_tag="VGC82_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0423876.1"
                     /translation="RKTGAIGFAALRGGQVVGEHSLIFAAGSEHIVLTHRAFDRRTFA
                     TGAVRAAIWSVSQPPGLYSMMDVLGMA"
BASE COUNT          447 a          845 c          824 g          426 t
ORIGIN      
        1 agcgacgcct gccgtcggtt accagatcgg atcgatgtga taagcgccag gccgaagggc
       61 ctctaagacc gttcatttgc gggtattctg ccattccgct tccggcccag atctcgccaa
      121 aatgcgaatg acggttatcc ccgagttgcg accaagagcg gaaagaccgg tgtccgccaa
      181 tcgcggccga ctgaccaaca tgggcgcctt gctgccgcga cggccgcgtg gcttggaacc
      241 ttcaaactga cccactacca gggcaagcgt acaggaaatc ggaggaacac gccgacatcc
      301 aagccattcc gcgtcctccg cgccgggctc atccgccgca atggccttta aaggccatca
      361 gcccacccgt tcgcgccggc gctggaagac ccagtagagc ggctggcgcc ctgcccgcaa
      421 tgccttggcc tcataccggg tcggtggcca gccgtccggc cgacccgtgt ggattgctac
      481 ggtatcgaac agcgtctgcg acgccatcac atcccgcacc caggcctgat aggtcggatc
      541 gtcgctggcc acgcgccatt cggcgccgaa cttcataacc cgggccagca gcggcagcat
      601 cgccggatgc acgaagcggc gcttggcgtg ccgagccttc ggccatgggt cggggaacat
      661 caggaacagt tggtcgaggc agccatccgg caaagcacgc agcagaatgc gggcatcgtc
      721 ggtccacagg cggaggttgg gcggcagcgg cgcggttgcc tcctcaccct cggggaccag
      781 tgccgagagc agcgagcaga tgccgttttc gaagacctcg cacgcgatca gcgtcacatc
      841 cggatgggca cgcacctggg caagtgcatg ttcgccgccg ccgaagccga cttcgagcca
      901 aagcgggccg gggaacggcg gcagcgggtc gggcaacgcc agacgcggca gggtcacatc
      961 cagcaggact tcctggcggg gccgcagctt gtggccgcgc gcccgcccgt ataaccggtc
     1021 cggtggcggc tttaccgccc gaaggccgcc ctcagcgccg gcaccagatc ggtcttttcc
     1081 cacgtgaagg tgcccttcgc ctcgtccggc tcgcgcccaa aatgaccgta cgccgaggtc
     1141 ggcgcataga tcgggcggtt catcttgaga tgttcgcgaa tgccgcgagg cgacaggttc
     1201 atcacatccc gcaacgtccg ctccaaccgg ctttcatcga tatcccggcc ggtgccatgc
     1261 aaatccacat acaccgacaa cgggcgggcc acgccgatcg cgtagctgat ctgcagggtg
     1321 cagcgatccg cgaggccggc ggcgaccacg ttcttcgcga ggtagcggca ggcataggca
     1381 gccgatcgat cgaccttggt cgggtccttg ccgctgaacg caccgccgcc gtgcggggcg
     1441 gcaccgccgt aggtatcgac gatgatcttg cgtccggtca ggccgcaatc gccgtcgggg
     1501 ccgccgatga cgaagttgcc ggtcgggttc acatggaact cctcctcggt gcacatccaa
     1561 cctttcggca ggatcgtctc caccagcggc cacagcaggc gcttgatctc aggctgctcc
     1621 agccccgcca tgtgctgggt ggacaccacg accgaggtcg cgcccgccgg cttgccatcg
     1681 acatagcgca gggtgacctg gcttttggcg tccggcagca gccccgctac cgagcggtcg
     1741 ccagccttgc gcagatcgcg gatggtgcgc aggatttcgt gcgcataata cagcggcgcc
     1801 ggcatcaagg tctcggtttc gcggcaggcg tagccgaaca tgatgccctg gtcgccagcg
     1861 ccctcgtcct tgttgccggc gctgtccacg ccctgggcga tgtcgctgga ctgcgagtgc
     1921 aggtacaccg cgacgtccgc gttggcccag tgaaagcctt cctgctcgta gccgatgtcg
     1981 cggatcgcca gccgcgtcag gtgagtcaga agctccggcg taaccgttgc cggcccgcgg
     2041 gtttcgccgg ccaggacgac gcggttggtc gtgacaagcg tttcacaggc gacgcgggca
     2101 taagggtcgg cagttaggaa ggcgtccagg accgtatcgc ttatccggtc ggcgaccttg
     2161 tcgggatggc cctcggaaac cgactccgag gtaaacaaat actcgccgtt gtcccgcatg
     2221 aggtcatttc tcgcattgag gtgggttgcg gcctgccaga tggcggcttt cggcagggtg
     2281 ccaaagttgg cggtgtgtgg ccgaatccgt cctcagggtc aagctttcaa gccatgccga
     2341 gaacatccat catcgaatag agcccgggcg gctgcgatac cgaccaaatt gccgccctga
     2401 ccgcacctgt tgcgaaggta cggcggtcga acgctcggtg ggtcaggacg atgtgctcgc
     2461 tgccggcggc gaagatcagg ctgtgctccc cgaccacctg gccgccgcga agcgcagcga
     2521 acccgatggc gccggtcttg cg
//
LOCUS       DASWQN010000656         3572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_62763, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000656 DASWQN010000000
VERSION     DASWQN010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62763"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VGC82_11190"
     CDS             complement(<1..426)
                     /locus_tag="VGC82_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0423877.1"
                     /translation="MDQPVRELPTGDWPAGLTRVPYWVYRDPVVARDEQARIFEGPSW
                     HYLCLEIEVPEVGDYRAAMMGAMPVVVARAENGDVVAFENRCAHRGSLICLDDSGNGK
                     DFQCVYHAWRYDLRGNVTSIAFRRGVNGKGGMPEDFDMSA"
     gene            643..1863
                     /locus_tag="VGC82_11195"
     CDS             643..1863
                     /locus_tag="VGC82_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0423878.1"
                     /translation="MSAYLVGLLAILLSAATALAAQVPAPNPAQGDGGVSSFYTWSGD
                     IPAAPGQMLRSEPLEPNLGLAMAGEQRRILYSSIDGIDGQTPITVSGAYFLPKGTPPA
                     GGWPLLAWAHGTTGLADTCAPSWNPRSERDATYLNSWLQQGYAVVATDYQGLGTPGPH
                     PYLVVRPEAYSVLDSVRAVLKSLPDIANRVVLIGQSQGGGAVFATAGVAPGYAPELNI
                     RGSVATGVPFLDPVMLRSRPPPNPQDKADPTVAYNLYIGLLVQQGNAALTADELVTER
                     ALPLLQEARTTCVGKLFRDVMAAGLNRGNALKPGFRAAFASVLPMMQYTTMRLPQPLF
                     VGTGEQDHDVPPATQLALVRNACASGSVVEAHLYAGLSHGATVNASLKDSVPFVRKVM
                     AGEPITPVCEPAAE"
     gene            complement(2039..3238)
                     /locus_tag="VGC82_11200"
     CDS             complement(2039..3238)
                     /locus_tag="VGC82_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0423879.1"
                     /translation="MADILIAGAGLGGLTMALALLQRGHQVRVFEQASELREVGAGVQ
                     LGANGTRILIALGLDAAMQRVVCAATGKEIRLGTTGQCWPTFDLGETSIERFGAPYWM
                     VHRGDFHRVLLDAVRSTDPDAVRAGNGCVGFEQSADHVTLHLANGEQATGEVLIGADG
                     VHSRVRQQMFGPGSGKAGSPHFTGIMAWRGLVPMQRLPPHLRRLVGANWIGVGGHVVT
                     YPLRRGEILNFVGVVERDDWRLESWNEPGSREECLHDLRNWHDDVKTIIRNIDTPYKW
                     ALLGREPLDHYAQGRVCVMGDAAHPTLPFLAQGANMALEDAIVIARCLDSFEVPEALR
                     RYENARLPRTAAIVRGSSDNTKRFHNPALGSPEGAAAYVEREFQPEKVQQRYDWLYQY
                     DALTVAL"
BASE COUNT          632 a         1164 c         1130 g          646 t
ORIGIN      
        1 cgccgacata tcgaaatctt ccggcatgcc gcccttgcca ttgacgcctc ggcggaaagc
       61 gatggaggtc acgttgccgc gcaaatcata gcgccaggcg tgataaacgc attggaagtc
      121 tttgccgttg ccgctgtcgt cgaggcagat cagcgagccg cgatgggcac accggttctc
      181 gaatgcgacc acatcgccgt tctcggcacg agcgaccacg acgggcatcg cccccatcat
      241 cgcagcgcga taatcgccga cttcagggac ctcgatctcc aggcacagat aatgccagct
      301 tggtccctca aatatccgcg cctgttcgtc acgcgcgacc acgggatcgc gatagaccca
      361 gtagggaacc cgggtcaggc ctgcaggcca gtctccggtc ggcagttctc gcacaggctg
      421 gtccatgttg ccctccctga ccgatttgcg cgacgctagg ccacttctcg gcgttctcgc
      481 aaatcagcgt ccggtgttcc cacgcctgcg agcataatgt cctgtgggca tcatgtcctg
      541 ggcatcatgt cctgggcatc attgatagaa gcgctcaatc taaactacgc ttcgccaaca
      601 cttgaaggca tgccggctgg aagttccagg gagaaccgga caatgagcgc atacctcgtg
      661 ggcctgttgg cgattttgct gtcggcggcg acggcgcttg cggcgcaggt gcccgcgcca
      721 aacccggcgc agggcgacgg cggcgtctca tcattctata cttggtcggg ggacatcccc
      781 gcggcaccag ggcagatgct gcgcagcgaa cctctcgagc cgaaccttgg actggcgatg
      841 gcgggtgagc aacggcgcat cctgtattcc tccatcgacg gaatcgacgg ccagacgccg
      901 atcaccgtgt cgggcgcgta tttcctgccg aaggggaccc cgccggcggg cggttggccg
      961 ttgttggcct gggcccacgg cacgaccggt ctggcggaca cttgtgcgcc gtcgtggaat
     1021 ccgcggtcgg agcgtgatgc gacctacctg aatagctggc ttcaacaagg ctacgccgtc
     1081 gtcgcgaccg actaccaggg gcttggtacc cccgggccgc acccgtacct cgtggtgcgg
     1141 ccggaggcgt acagcgtact ggacagcgtg cgcgcggtgc tgaaaagctt gccggatatc
     1201 gcgaaccgcg ttgtcctgat tggccagtcg cagggcggcg gtgcggtatt cgccactgcg
     1261 ggcgtggccc cggggtatgc gcccgaactg aatattcgcg gcagcgtggc cactggcgta
     1321 ccgtttcttg atccggtcat gttgcgttcg cgaccgccgc caaacccgca agacaaggcc
     1381 gacccaacag ttgcctacaa cctctacatc gggcttctgg tgcagcaggg caacgcggcg
     1441 ctgacggcgg acgagttggt gaccgaacgg gcgctgccgt tgctgcagga ggcgcgtacc
     1501 acctgcgtcg ggaaattgtt ccgcgacgtc atggccgccg gtctcaaccg gggcaatgcg
     1561 ctaaaacccg gcttccgggc ggccttcgct tccgtcctgc cgatgatgca gtacaccacc
     1621 atgaggctgc cgcaaccgct gttcgtcggc accggcgaac aggatcacga cgtgccgccg
     1681 gcaacccaac tcgccctggt tcggaatgct tgcgccagcg gatcggtggt ggaggcgcat
     1741 ctttatgccg ggctcagtca cggcgccacg gtgaacgcct cgttgaagga ttcggtgcct
     1801 tttgttcgca aagtcatggc tggcgaaccg ataacgccgg tttgcgagcc ggccgcggaa
     1861 taaacgctgc cgccccatct ggcccacggc ggcgggtatg aagaagccac cctgggttta
     1921 ggcttccgcc cgcgatgcca aagcattttc agcatgacgt gcatggtgaa atgctccggt
     1981 cccttgttcg atcgcatgat tcacccgctt caaacgttcc gctcagcgcg atcatgccct
     2041 aaagtgccac ggtcagcgcg tcgtactggt atagccagtc gtagcgctgc tgaacctttt
     2101 ccggttggaa ctcccgttcc acataagcgg cggcgccttc gggactgcca agtgccgggt
     2161 tgtggaagcg cttcgtgttg tcggacgatc cgcgcacgat cgccgcggtg cgtggcagac
     2221 gggcgttttc gtagcgccgc agcgcctcgg gcacctcgaa cgagtcgagg cagcgggcga
     2281 tgacgatggc gtcttccaaa gccatgttcg cgccctgcgc caggaacggc aatgttggat
     2341 gcgcggcatc gcccatcacg cacacccgcc cttgcgcata atgatccagc ggttcacgtc
     2401 ccagcagggc ccatttatag ggcgtatcga tgttgcggat gatggtcttc acgtcgtcat
     2461 gccagttgcg gaggtcgtgc aggcactcct cccggctgcc tggctcgttc caggactcca
     2521 ggcgccagtc gtcacgctcc accacgccga cgaagttcag gatttcgccc cgccgcagcg
     2581 gataggtcac cacgtggccg ccgacgccga tccagttggc gccgaccagg cgccgcaggt
     2641 gcggcggcag acgctgcatc ggcaccaggc cgcgccaggc catgatgccg gtgaaatgcg
     2701 gcgaacccgc cttgcccgat cctggtccga acatctgctg gcggacgcgc gagtgtacgc
     2761 cgtcggcacc gatcagcacc tcgccggtcg cctgctcgcc gttggccagg tgcagggtga
     2821 cgtgatcggc gctctgctcg aaccccacgc acccgttgcc ggcgcgcacc gcatccggat
     2881 ccgtggagcg tacggcgtcc agcagcaccc tgtggaagtc gccacgatgc accatccaat
     2941 agggtgcgcc gaatcgttcg atggaggttt cgcccaggtc gaaggttggc cagcattgtc
     3001 ccgtcgtgcc gagccggatc tccttgccgg ttgcggcgca gacgacacgt tgcatggccg
     3061 catcgagccc aagggcgatc aggatccggg tgccgttggc accaagctgc acgcccgcgc
     3121 cgacctcccg cagttcactg gcctgctcga acacgcgcac ctggtgtccg cgctgcagca
     3181 gcgccagcgc catggtcagc ccgcccaggc cagctcccgc gatgaggata tccgccatag
     3241 gtttcccccg agttgccgat cccgcggtca cagtatccaa gcgcggtccg aacgcaaacc
     3301 ttggtcgagg ttgagcggca tggcatcatt ctgtgatacg gaagcatcga tcgatgggct
     3361 ggtacacccg acgctcggaa ccaggccgcg gaaatcggac gccggggggt gatccttgca
     3421 ggtgggatca tcggaaaggt gaatcatgcc ctcgaacaaa cggcttaggg cggtgtctgc
     3481 ggaccaaggt agagcagaaa ccgctctgat gaatgaaatc tggcggaagg cgccgtccga
     3541 cccatccatc aggcgtcgca atcgcacgag ga
//
LOCUS       DASWQN010000657         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2305317, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000657 DASWQN010000000
VERSION     DASWQN010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2305317"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..601)
                     /locus_tag="VGC82_11205"
     CDS             complement(239..601)
                     /locus_tag="VGC82_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423880.1"
                     /translation="MQIGANHNRSPAPVRFLQVNKAELGKIVKTSTDPVIERIAAYPW
                     PRGSVSVRKLRGGYSLSSARTGAPVARLKPMPQDDRFEVLWWRRDAWGSAGPFGAGLS
                     LNDALAFIASEPPFWIRA"
     gene            581..>1542
                     /locus_tag="VGC82_11210"
     CDS             581..>1542
                     /locus_tag="VGC82_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0423881.1"
                     /translation="MVRPDLHLPQAFVAWMAPFLTAFCQRSRDTAAALAVGALMAIGP
                     RTVTNCLRALGLAEHPSFTAFHRVLNRNVWSSLALARTLLRLLVPVFEPGRPFVIIGV
                     DHTLERRRGRRIEPASHFHDAVRSTAKQKVTSRGLRWISAMLLVNVPFAARIWGLPVL
                     TALTPSKAWCESHKRRYRPVTEWAERLLLTLHRWLPDRMIVAVMDGEFAAIALLHRLR
                     QSMVVITRLRLDACLFDPPPPYKGRGRHPKKGPRQPSLKARITDRATVWKRAVQASRT
                     SWRSGGWIEYASGTALWWHAGKEPLPIRWILVRYPDKRHGTEAFL"
BASE COUNT          266 a          508 c          480 g          288 t
ORIGIN      
        1 agtggcgggt ggatcgagta tgcgtccgga acggcgctgt ggtaccacgc tggcaaggag
       61 ccgctgccaa ttcgctggat actggttcgt tatccagaca aacgtcacgg tacggaggct
      121 tttctatgca ctgacaccca aatggccctg cttgacgtgc tcgactgcta caatcgccgc
      181 tggtcgatgg aaacgactta tgaagaagct ggactttgcc catcacgggc cagtgagatc
      241 aggcccggat ccagaagggc ggctccgagg cgatgaaggc gagagcgtcg ttgagcgata
      301 atccggcgcc aaacgggccg gccgagcccc aggcatcacg gcgccaccac agtacctcga
      361 agcggtcatc ctgcggcatc ggcttcagtc tggcgacagg cgcgccggtg cgggcagatg
      421 acaagctata gccgccgcgg agcttgcgga cgctgacgct gccgcgcggc caagggtagg
      481 ccgcgatccg ctcgatcaca ggatcagttg atgtcttaac gatcttccca agctccgctt
      541 tgttgacctg cagaaaccgg accggcgcag gagatctatt atggttcgcc ccgatctgca
      601 tctgccgcaa gccttcgttg cttggatggc acccttcctc accgccttct gccaacgcag
      661 tcgcgacacc gccgcggccc tggcagtcgg cgccctgatg gcgatcgggc cgcgcaccgt
      721 caccaactgc ctgcgcgccc tcggcttggc cgaacatcca agcttcaccg cctttcatcg
      781 cgtgctcaac cgcaatgtct ggtccagctt ggcgctggcc cggaccttgc tgcggttgct
      841 ggtcccggtg tttgagccgg gtcggccatt cgtcatcatc ggcgtcgatc ataccctgga
      901 acgccgccgt ggtcgccgca tcgagcccgc cagccatttc cacgatgccg tccgctccac
      961 cgccaagcag aaagtcacca gtcggggcct gcgctggatc agcgcgatgc tgttggttaa
     1021 cgtgccgttc gccgctcgca tctggggact accggtgctg accgcactga cgccgtccaa
     1081 agcctggtgc gagagccaca aacggcgtta tcggccggtg accgaatggg cggagcgtct
     1141 gctgctgacg ctgcatcgct ggctgccgga ccgcatgatc gtggctgtca tggacggcga
     1201 attcgccgcc atcgctctat tgcaccggct gcgtcagtcc atggttgtca tcacccgcct
     1261 gcggctggat gcctgcctgt tcgatcctcc cccgccctac aagggacgcg gccgccatcc
     1321 caagaagggc ccgcggcaac cgtcgctgaa ggcgcgcatc accgaccgtg ccacggtctg
     1381 gaagcgagcg gttcaggcca gccgcacgtc ctggcgcagt ggcggctgga tcgagtatgc
     1441 gtccggaaca gcgctgtggt ggcacgccgg caaggagccg ctgccgattc gctggatact
     1501 ggttcgttat ccagacaaac gtcacggtac ggaggctttt ct
//
LOCUS       DASWQN010000658         1812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1382304, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000658 DASWQN010000000
VERSION     DASWQN010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382304"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VGC82_11215"
     CDS             <1..181
                     /locus_tag="VGC82_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423882.1"
                     /translation="RRAKEKDDSISHFMVDASATAALAYGRAAGVYTVDKDAGAGESS
                     CAPARDGHVLHERAG"
     gene            complement(383..459)
                     /locus_tag="VGC82_11220"
     tRNA            complement(383..459)
                     /locus_tag="VGC82_11220"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(423..425),aa:Pro,seq:tgg)
     gene            complement(523..1005)
                     /locus_tag="VGC82_11225"
     CDS             complement(523..1005)
                     /locus_tag="VGC82_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY0423883.1"
                     /translation="MNKTADPHRQYSWLRMFLALATAICVFSGGAEAASIQPLEIVTR
                     NGVQVFSVEMATTEQEKETGLMYRKELADGKGMLFDFSPEQQVSMWMKNTYIPLDMIF
                     IRADGRILRIAENTEPLSTRIIASGGLAKGVLEVIGGTARKYGIEPGDRVAHPLFNGR
                     "
     gene            complement(1143..>1812)
                     /locus_tag="VGC82_11230"
     CDS             complement(1143..>1812)
                     /locus_tag="VGC82_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY0423884.1"
                     /translation="GVMGSDGFESKKLGGASAVTLANRLGPGELSGGAPLDPTLDALS
                     GLGDSAANLVEISGVIKWFDASKGYGFIVPDNGWPDVLLHVTVLRRDGYQTAYEGARL
                     VCECVQRAKGYQAFRILSMDESTAIHPAQMLPPRTHVSVTPTSGLERAQVKWFNRLRG
                     FGFLTCGEGTPDIFVHMETLRRFGMTELRPGQYVLVRFGPGSKGMMAAEIQPEAGSPG
                     LSSH"
BASE COUNT          376 a          575 c          531 g          330 t
ORIGIN      
        1 aagacgggcc aaggaaaaag atgatagcat ctctcacttc atggtcgatg cgtccgctac
       61 cgcggcgctt gcttacggtc gagcagcggg agtctacacc gtggacaagg atgcaggagc
      121 tggggagagc agctgtgcac ctgctcgcga cggccacgtc ctgcatgaac gggcaggatg
      181 aatgatggtc cgcgcaattc cggaccagat agaggaatca cggctccctc tcggtgcccg
      241 gccagaccgc cacagagcgt gcaagggtcg ttttcatctg cccgaaatgt gctgtggtcg
      301 atcgagccca agcgactttg gcaaagccag cgggagacgg ttcaattgtc ccgattgcgg
      361 aacaagtgtc tatgcttggg catggtcggg gcagctggat tcgaaccaac gacctgcagt
      421 acccaaaact gccgcgctac caggctgcgc tataccccga tgatccaagg aaaagatgtc
      481 gatacacgct taaagcgctg ccagcaaggc ttcggacgcc gttcagcggc cattgaagag
      541 cggatgcgcg acccggtcac caggctcgat accgtatttc ctggcggtac cgccgatcac
      601 ctccagaacc cccttcgcca agccgcctga cgcaatgatc ctggtggaca aaggctcggt
      661 attctcggca attcggagga tccggccgtc cgcgcggatg aagatcatgt cgagcgggat
      721 gtaggtattt ttcatccaca tcgagacctg ctgttccgga gagaaatcga acagcatgcc
      781 tttgccgtcc gccagctcct tgcggtacat caggccggtt tccttctcct gctcggtcgt
      841 cgccatctcg accgagaaaa cctgcacacc attcctggtg acgatctcga gcggctggat
      901 gctcgccgcc tcggctccgc ccgagaagac gcaaatcgcg gtcgctagcg ctaaaaacat
      961 ccgcagccac gaatattggc gatgcggatc ggctgttttg ttcatggaac cgcttgaccg
     1021 gccgagttgg aattcgtgat gccgacccta ccccgaggat ccgggcaaat accagaccgg
     1081 atccggcgtc cggcgatgcc gttttcggca tccggtgatg tcgggaagcc gcaagctgcg
     1141 cgttagtgcg acgacagccc gggcgagccg gcttccggct gtatttcggc ggccatcatg
     1201 cccttggagc cgggcccgaa ccgcaccaga acatattggc cagggcgcaa ttcggtcatg
     1261 ccgaaccgcc gcagcgtttc catatggacg aagatatccg gcgtcccctc cccacaggtc
     1321 aggaagccga atccgcgcaa gcggttgaac cacttgacct gggcccgctc gaggccgctg
     1381 gtcggggtca cgctgacatg ggtccgcggc ggcagcatct gcgccggatg gatcgcagtc
     1441 gactcgtcca tcgacaggat tcgaaacgcc tggtaaccct tcgcgcgctg tacgcactcg
     1501 cagaccagcc gcgcaccttc atacgcggtc tgatagccgt cgcgccgcag cacggtgaca
     1561 tgcagaagca catccggcca accattgtcg ggaacgatga agccgtaccc cttcgaagca
     1621 tcgaaccatt tgattacgcc gctgatttcg acaaggtttg cggccgaatc gccgagaccc
     1681 gataacgcat cgagcgtcgg gtcgagcggc gcgccgcctg acaactcacc cggtcccaac
     1741 cggttcgcta acgtaacggc agacgcgccg ccaagcttct tggactcaaa tccgtccgac
     1801 cccatgaccc cg
//
LOCUS       DASWQN010000659         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_985779, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000659 DASWQN010000000
VERSION     DASWQN010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985779"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1146)
                     /locus_tag="VGC82_11235"
     CDS             complement(223..1146)
                     /locus_tag="VGC82_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0423885.1"
                     /translation="MEMTTALIKSGRIVARLRYSIRGNLLLALIALLTLGTATMAASS
                     LNANKASVLGPQTMHLAPAIGQNELQELPVPSKAFPPAGLSRADRAQVVTPRVIDAIR
                     VAQQKSHVDPGLLLAIAAKESGFNPEARNRHSTARGLLQFTNATWLTVVRDFGPRYGL
                     SRYAQAIETDRDGNLRVSKPRLRRAILALRDNPRLQAIMTAERLEQGRPTLELHLGRS
                     ARAADLYFLHLLGPTGATHFLTELNEHPDASSLTVVGGAAKANAGLFVRDGRPLTVAE
                     AYASVQATLDEQASRHADLFQPRPMLVSDAR"
     gene            complement(1355..1783)
                     /locus_tag="VGC82_11240"
     CDS             complement(1355..1783)
                     /locus_tag="VGC82_11240"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEY0423886.1"
                     /translation="MPINSLPDHAVTTTPSAAMRSPPALWAGLRRGLARKCPACGQGQ
                     LFRGYLAVIPVCPVCANDNEQYPSDDFAPYVTIFLVLHLMVPILFAADRTWDMSVGLE
                     MALALPLFLIATLLLLPFAKGAVIGFAWARGVTRNPAANP"
BASE COUNT          412 a          628 c          615 g          377 t
ORIGIN      
        1 aattcaagcc ctgaagagct tgcgtgggag tcccgtgtgg cggcacccac gatgttcctc
       61 tgacgcaaaa tcgagggcgg cggcgtcagg catggccatg acactatcgt caaccgctgc
      121 agccttcgtc accacgccaa cgccaatttt gcgatgtatc cctgcctgga tgccgtccat
      181 cccacagccg ccttatgcgc atcactcacg agcattggcg ccttatcgcg catcgctcac
      241 gagcattggc cttggctgaa acaggtcggc atgtctcgac gcctgttcgt ccagcgtcgc
      301 ttgaacagag gcataagcct cggcaaccgt cagcgggcgc ccgtccctga cgaatagccc
      361 ggcattggcc ttggccgcac cgccgacgac ggtcaggctc gacgcatcgg gatgctcgtt
      421 caattccgtc aagaaatgcg tggcgccagt tggaccgagc aggtgaagga aataaagatc
      481 agccgcacga gccgaacggc ccaggtgaag ctcaagcgtt ggccgccctt gttccagtcg
      541 ctccgccgtc atgatcgcct ggaggcgcgg gttatcgcgc agggccagga tcgcgcgacg
      601 aaggcgaggt ttgctgaccc gcagattccc gtctcgatcc gtttctatcg cctgcgcgta
      661 gcgcgacagt ccgtatcgtg gcccaaaatc cctcaccacc gtcagccatg tagcattggt
      721 aaactggagc agtccgcgtg cggtggaatg ccgattgcga gcctcggggt tgaagccgct
      781 ttccttcgcg gcgatagcaa gcagaagacc gggatcgaca tggcttttct gctgggcaac
      841 ccggatggcg tcgattaccc gaggcgtgac gacctgcgcc cgatcagcac gcgatagccc
      901 ggcgggcgga aatgccttgc tcggaacagg cagttcttgc agttcgtttt gaccaatggc
      961 cggtgcaaga tgcatggttt gagggccaag tacagacgcc ttgtttgcgt tgagactcga
     1021 ggctgccatg gtcgccgtcc caagagtgag cagggcgatc aacgccaaca acaggttgcc
     1081 gcgaatggaa taacggagcc gcgcgacaat ccgtcctgat ttaatcagcg cggtggtcat
     1141 ctccattatc tacctttccc gcactttgac tggcgctcct tgacacggct gatgaaaagc
     1201 ggttcccggc cgctacccca cataatcccg ggcgccagac gtatctaaag gttggaagcg
     1261 tcttcccaga tacaagtccg accgattttc gtggaacccg ctgctttgaa tcaccccgca
     1321 ccctgttccg accgcctcat gggcttcgat cgcctcaagg attggcggcc ggattgcggg
     1381 taacgccacg ggcccaggcg aaaccaatca ccgcaccctt ggcgaacggc agcagcaaaa
     1441 gcgtcgctat caggaaaagc ggcagcgcca acgccatctc gaggccaact gacatgtccc
     1501 aggtacgatc cgcggcaaac aggatcggga ccatcaggtg cagaaccagg aagatggtga
     1561 catacggggc aaaatcatcc gacgggtatt gctcgttgtc gttggcgcag accggacaaa
     1621 ccggaatgac cgcgagatac ccgcggaaca attggccctg gccgcaggcc gggcatttcc
     1681 tggccagacc gcgccgcaaa cctgcccata gggcgggcgg cgagcgcatg gccgcggaag
     1741 gcgtggtggt gaccgcatgg tcgggcagcg agttgatcgg cacgtccggt actcctgacg
     1801 agcctactta actgaactgc acaagatttg ttcccgcgcc tcgggcccga gcgaggctgg
     1861 atggatgcca acaacatgtc gccgattcca tctggtggcg agtcgtcagg cgcttgggca
     1921 tgtgccaatc agcgtccgga ccggcatgac gtcgttctgg cacgacgtcg tcgtcgagga
     1981 tggtgatcct taccttggat gcgcggcgag caacgcccga ccgcgctggg tc
//
LOCUS       DASWQN010000660         7067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2305544, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000660 DASWQN010000000
VERSION     DASWQN010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2305544"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VGC82_11245"
     CDS             complement(<1..415)
                     /locus_tag="VGC82_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0423887.1"
                     /translation="MSFASAPSQPGAISRPGGSYRPACSVTHLVLIPSFNSGRLLQDT
                     VAAARACWTPVWVVIDGSTDDSAAVITSMAEADLGLRVLRLPSNQGKGSAVRHGLAAA
                     QARGFTHALVMDADGQHPAGSIATFMATSASAPDAI"
     gene            718..1035
                     /locus_tag="VGC82_11250"
     CDS             718..1035
                     /locus_tag="VGC82_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY0423888.1"
                     /translation="MNVVTKVTAGERSPSEDPHPPSAPRVLALAQLIIETLNLDISAS
                     TIDPQAPLFGEGLGLDSIDMLEISLAVSQTYGVKLRADDSDNHQIFRSLASLSEHIHQ
                     HTG"
     gene            1039..1686
                     /locus_tag="VGC82_11255"
     CDS             1039..1686
                     /locus_tag="VGC82_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423889.1"
                     /translation="MRGGIVGGGFLILAGIAAFLIHQDLTGLTPAWLVGAAVAMQAAA
                     VWFISRRLAFRYRGIPAVVIILILAAVFLFGLPVRSVGLASGGMCHAAAYVGLLTWFA
                     GSLRPGQEPAVTGFARRIRRTMPDKVVRYTRWVTIAWCAFFAAQIGISALLLTMASQT
                     VWIAFVNLWNLPLVVAMIVAELGVRLLLFRREPRTGLLDTLYGLHRLRARPGSLP"
     gene            1683..3341
                     /locus_tag="VGC82_11260"
     CDS             1683..3341
                     /locus_tag="VGC82_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0423890.1"
                     /translation="MSGAIGTRLPILCDGDRPVARRGSRIIPRSGFLAEIEELAERLP
                     DWPYVVNFCSDRYHFTVAWAASMLRGQVTLLPASRDAAAVAALRADYQALYVLVDDDA
                     ATDHSAPHFAYPALAQAGASSRVPAFAPDQIAAVLFTSGSTGLPNPSPRRWGRLVAGS
                     RAAGAALGVGRFPGAALVATVPHAHSYGLESAVMLPLQHGLLMTAERPFFPADIIASL
                     ESGDLPGILVTTPVHLRALVGDAAGAGFGTSFRAAFVLSATAPLSGELAERAEAAFRA
                     PVQEIYGCSEAGQLATRRTIEGPTWRCLDGFRLYNDAAGCWASGPHESDVLLADEIET
                     IKDGGFVLHGRTADLVNVAGKRSSLAYLTRQLLAIDGVEDGAFLIPEDVAAGATSRLA
                     AVALAPGLTPGTILDRLRERIDAAFLPRPLHVVDTLPRNGLGKLPRSEILRLIGSTKP
                     AAAHPAVIRLHFRVDHPTVAGHFPGNPIVPGAVLLDELVAALFPDGWSGVVEAAKFHH
                     PVRPGDTLTVARQTEGEQTRFKCFLAETEQLVLSGILRTPSPSL"
     gene            3392..4234
                     /locus_tag="VGC82_11265"
     CDS             3392..4234
                     /locus_tag="VGC82_11265"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423891.1"
                     /translation="MTVLRSAVWLARSLGRPVTRLLILPVCLGFALFHAEARTASNRY
                     LARVLGRRPRALEVIRHFFTFGTTIVDRVYLLNDEAERFDITIHGEEILTEILARGEG
                     CLLLGAHHGSFEVLRLVGRRQPRLSVSLAMYEETGRKIGAALNAINPRLTPSIIGLGT
                     PSAMIEIKDCLEQGGFVGMLADRTLRTDTPIQVDFLGARAAFPTGPARLATLLRPQVV
                     LMVGLFRGGNRYDIHFERLTAAGAAKPGPEEATRLIKNYVARLEHFCRLAPYNWFNFF
                     DFWA"
     gene            4236..4838
                     /locus_tag="VGC82_11270"
     CDS             4236..4838
                     /locus_tag="VGC82_11270"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HEY0423892.1"
                     /translation="MNRLRRMVLLGFLTTFPAHAAQTASALPVTLDQLMATLHSVRHV
                     DARYTERRYLHILRTPVETRGSLRFDAPAHLEKATDPTANGVAERLTIDGDRLTIDRG
                     GGVSPIVLILHEHPEIGVLVESIRATLSGDGEALRRIFDITPSGTIDHWQLVLQPRDR
                     SQHALLQWMRISGYAGRITTIDTANDQGDRSEMAIVEQTP"
     gene            4835..>7067
                     /locus_tag="VGC82_11275"
     CDS             4835..>7067
                     /locus_tag="VGC82_11275"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0423893.1"
                     /translation="MIVRRVAVLTWIVLLAGACLFVAQLHIDTDMTAFLPRSASPAQR
                     VLIEQFRDGVVSRLLLVAIEGVPADRATALSKLLAGRLRGNGEFVAIENGEESGFARD
                     RDFLWSNRYLLSDRIEAGRFTRAGLRGALEHDLAGLGSASGFLVERTLAADPTGEMLH
                     LVEQQMEGGNGPARHDGVWVSPDGSRALMLVRLRAAGSDIDGAQRALATVQSAFDKAA
                     SDPADQAARLVMTGPPVFAVQARDRIKADATRLSALASLLVATALLVVYRSPRVLALA
                     FVPVVSGAVIGIAAVGLVFRTVHGITLGFGATLIGEAVDYAVYLFTQTSPNSPVQPTM
                     GRIWPTLRLGVLTSICGFSAMLLSSFEGFAQLGLFTITGLAVAVCVTRWVLPALIPER
                     FAGVRQSRIMMRIATFAAGLRRLRIGVLVLAATATGALAMSKGAFWAGDLASLSPVPP
                     AEQRLDQSLRRDMRAPDAAYLIVVRRADQEAALQAAERLTTSLVPLVAQGALSGIDSP
                     ARYLPSLAAQRSRQAVLPDPAILQVELDAALDDLPFQPGLFAPFIAAIGNARSGSPIT
                     RTELDGTSLSLKLDSLLLHRQDGWVAMLSLRGVTAPDVIATAVAGEPDTVFVDLKAES
                     DRLLGVYLREGISLAGLGAAAIGLLLAVSLRSLPRLAAVTAPLVASVLVTLAILRLGG
                     HVLSVFNLFGMLLVVAIGSNYCLFFDRQRGDPAGMPRVLASLLLANACTVAGFGVLAL
                     SHTP"
BASE COUNT         1059 a         2307 c         2284 g         1417 t
ORIGIN      
        1 cgatcgcgtc tggagcggag gcggaggtgg ccatgaaggt tgcgatgctt ccggccgggt
       61 gttgcccgtc ggcatccata accagggcat gggtaaaccc acgcgcctgt gccgcggcta
      121 agccatgccg gaccgctgac cctttcccct gattgctggg caggcggagc acccgtaatc
      181 ccaggtccgc ttcggccatc gacgtgatca cagcggcgga gtcatccgtg ctgccgtcga
      241 ttaccaccca gacgggtgtc cagcaggccc ttgccgcggc aaccgtgtcc tgaagcagtc
      301 gtccgctgtt gaagctggga atgagcacca ggtgggtgac cgagcaggca ggacgatagg
      361 aaccaccggg cctggaaatg gcgcccggtt ggcttggagc cgacgcgaag ctcacggcgc
      421 gcccgcttcc tcggcctgcc ggcgacagcg cgcggtcgcc gatgcggcgg aggcggatgg
      481 catcctggtc tttcgcacgc aggcacctcc cacccaatcg aaccatccag ctcgtaggcg
      541 gccacgcgat tgggtgccac gagattaaat tgacgagcca agtcatcatc ttggcgcgga
      601 cgatcccggc atgcaaacct cgaatcgaac gagcgctgcg gtggcagcca gccaaaaaag
      661 cggtttaagt tcaggccgtt tctgctatgt cgggcgccga tctgaatttg cctggggatg
      721 aacgttgtga cgaaagtaac cgctggagaa cgatccccgt cggaagatcc gcaccccccg
      781 tctgcgccgc gggttctggc tcttgcacag cttatcatag aaacgctgaa cctggacatc
      841 agcgcgtcga cgatcgatcc gcaagcgccg cttttcggcg aaggcctggg gctcgattcg
      901 atcgacatgc tcgagatctc gcttgcggta tcgcagacct atggcgtgaa gctccgggcc
      961 gacgacagcg ataaccatca gatcttccgt tccctcgcca gtctcagcga acatatccac
     1021 cagcacacgg gctgaacgat gcgtggcggc atcgtcggtg gcgggttcct tatcctggct
     1081 ggtatcgcag catttctgat ccaccaagat ctgaccggcc tgacgccggc gtggcttgta
     1141 ggcgccgccg tcgcaatgca ggcggcggcc gtatggttca tatccaggag actggcgttt
     1201 cgataccgcg gcatcccggc agtcgtcatt attttgattc tggcggcagt ttttctgttt
     1261 ggactgccgg tgcgttctgt cggccttgcg tccggcggaa tgtgccacgc cgcggcttat
     1321 gtcgggctgt tgacctggtt cgccgggtcc ttgcgtcccg gccaggagcc tgccgttacc
     1381 ggttttgccc gccggatacg acggacgatg ccggataagg tcgttcgcta cacccgctgg
     1441 gtgaccatcg cctggtgcgc cttcttcgcc gcccagatcg ggatttccgc cttgctgctg
     1501 accatggcgt cgcaaaccgt ctggatagcg ttcgtcaacc tgtggaacct gccgctcgtg
     1561 gtggccatga tcgtggcgga attgggtgtc cggttgctcc tgtttcggcg ggagccgcga
     1621 accggcctgc tcgatacatt gtacggcctg caccgcctcc gcgcccgccc tggaagcctg
     1681 ccatgagcgg cgccattgga acccggctgc cgatcctgtg cgacggtgat cgccctgtcg
     1741 ctcggcgagg ctcgcgcatc attccacggt ccggatttct ggccgaaatc gaggaactgg
     1801 ccgagcgact gccggactgg ccctatgtgg tcaacttctg ctccgatcgt taccatttca
     1861 ctgtcgcctg ggcggcatcc atgctgcgcg gacaggttac gttgctgccg gccagtcgtg
     1921 acgccgctgc ggtggcggcg ctgcgggcag actatcaggc gctgtatgtt ctggtggatg
     1981 acgacgcggc gaccgaccat tccgcgccgc attttgccta tcccgcgctc gcgcaggcag
     2041 gcgcgagcag ccgcgtgccg gcctttgcgc cggaccagat cgccgcggtg ctttttacgt
     2101 ccggctcgac cggccttccc aatcccagtc cacgccgctg ggggcggctc gttgccggca
     2161 gccgagctgc gggtgcagcg cttggagtcg gacgattccc cggtgcggcg cttgtcgcga
     2221 cggtcccgca cgcccacagc tatggattgg aatcggccgt gatgctgcct ttgcagcatg
     2281 ggcttttgat gacggcggag cggccgttct tccccgccga catcatcgct tccttggaaa
     2341 gtggcgattt gcccggcatc ctggtgacaa ccccggtgca tctgcgggcg ctggtgggtg
     2401 acgctgccgg cgctggcttc ggcacctcgt tccgcgccgc gttcgttcta tcggccaccg
     2461 ctcccttgtc cggggaactc gcggagcgtg ccgaagcggc gttccgcgcg ccggttcagg
     2521 agatctatgg ctgctccgag gctggccaac tcgctacccg ccgcactatc gaaggaccga
     2581 cctggcgctg cctggacggt ttccgccttt acaacgatgc cgctggctgc tgggcttccg
     2641 ggcctcacga aagcgacgtc ctgctcgccg atgagatcga gacgatcaag gacggcggat
     2701 tcgtcctgca cggccgtacc gccgatctgg tcaatgtggc cggcaagcgc agttcgcttg
     2761 cttatctgac acgtcagctt cttgcgatcg atggggtcga agacggtgcc ttcctgatcc
     2821 ccgaggacgt tgcggcgggg gccacttcgc gactggccgc cgtcgcgctc gcgccaggtt
     2881 tgacgccggg gacgatcttg gaccggttgc gcgagcgcat cgacgctgcg ttcctgccgc
     2941 gtccgctgca tgtcgtcgat actctgccgc gcaacggcct aggcaagctg ccacgctcgg
     3001 aaatcctccg cctgataggt tcgaccaaac ccgcggcggc gcatccggcc gttatccggc
     3061 tgcacttccg cgtggaccat ccgacagtcg ccggccattt ccccggcaat ccgatcgttc
     3121 ctggcgctgt actcctggac gagttggtgg ctgccctttt ccccgatggc tggtcgggcg
     3181 tggtcgaagc cgcgaaattc catcatccgg tcaggccggg cgataccctg acggttgcgc
     3241 ggcagaccga aggcgagcag acccgcttca aatgcttctt ggcggaaacc gaacaactgg
     3301 tgctttccgg catcctgagg accccatcac catcgctgta aacacaaggc gccgttcgcc
     3361 ggactggagc agccgtcgcg agatcggttc gatgaccgtc ctgcggtctg cggtctggct
     3421 ggcccggagc ctcggccggc cggtgacccg cctgctgatc ctgccggtct gcctcggctt
     3481 cgccctgttc cacgccgagg cacgcaccgc ctcgaaccgt taccttgccc gggtgcttgg
     3541 ccggcggccg cgagcattgg aggtaatcag gcacttcttc acgttcggaa ctacgatcgt
     3601 ggaccgggtt tacctgctca acgatgaggc cgagcgtttc gatatcacaa tccacggcga
     3661 ggagatcctg accgagatcc tcgcacgggg cgaaggttgc ctgcttctcg gtgctcacca
     3721 cggcagtttc gaggtcctgc ggctggtcgg gcgcaggcaa ccccggctga gcgtcagcct
     3781 tgccatgtac gaggaaaccg gccgcaagat cggtgccgca ttgaatgcga tcaatccacg
     3841 tctgacaccc agcatcatcg gcctcgggac acccagcgcg atgatcgaga tcaaggactg
     3901 tctggagcag gggggcttcg tcggcatgct ggccgaccgg acactgcgca cggacacgcc
     3961 catccaggtt gattttctcg gcgctcgagc ggcgtttccg accggtcctg cccggcttgc
     4021 cacacttctg cggccccagg tggtactgat ggtcggcctg tttcgtggcg ggaatcgcta
     4081 tgacatccat tttgagcgcc tgaccgcggc cggcgccgcg aagccgggcc cggaagaagc
     4141 gacccgcttg atcaaaaact atgtcgcccg gcttgaacat ttctgccggc tcgcacctta
     4201 caactggttc aatttctttg atttctgggc atagcatgaa ccggcttcgg cgcatggtcc
     4261 tgctgggctt tttgacaacg tttccggctc atgccgctca gaccgccagc gccctgccgg
     4321 tcacattgga ccaacttatg gccaccctgc acagcgtccg gcacgtcgat gcccgctaca
     4381 ccgaacggcg ctacctgcac atcttgcgca cccccgtcga gacgcgtggt tccctgcgct
     4441 tcgacgcgcc ggcccatctg gaaaaagcga cggatcccac cgccaatggc gttgccgaac
     4501 ggctaaccat cgacggcgac cggttgacta tcgatcgggg gggtggcgtt tcgccgatcg
     4561 tcctcatact gcatgaacat cccgagatcg gcgtgctcgt ggaaagcatc cgtgccacgc
     4621 tgtccggcga tggcgaggcg cttcggcgta tcttcgacat cacgccgtcg ggcacgatcg
     4681 atcattggca gctcgtgttg cagccgcgcg atcggagcca gcacgcgttg ctgcaatgga
     4741 tgcggatcag cggctatgca ggacgcatca ctacaatcga cacagcgaac gatcagggcg
     4801 accgttctga aatggccatc gtggagcaga ctccgtgatc gtgcgccgcg tcgcggtctt
     4861 gacctggatc gtcctgctcg ccggcgcatg cctttttgtt gcgcagttgc acatcgacac
     4921 cgacatgaca gccttcttgc ctcgctcggc atcgcctgcg caacgggtgc tgatcgagca
     4981 gttccgtgac ggcgtggttt cccgtctcct gctggtcgcc atagaaggag ttcccgccga
     5041 ccgcgccacg gcgctcagca agctcctggc gggacggctg cgcggcaacg gcgagtttgt
     5101 cgcgatcgaa aacggcgagg agagcggttt tgcccgcgac cgcgacttcc tgtggtcgaa
     5161 ccggtatctg ctcagcgaca ggatcgaggc gggccggttc acccgcgccg gcctgcgggg
     5221 cgcactagag cacgatctgg ccggcctcgg ttcggcctcg ggtttcctgg tggagcgcac
     5281 attggctgcc gaccccaccg gcgagatgct gcatctggtc gagcagcaga tggaaggcgg
     5341 caacggtccg gcccgtcacg atggtgtgtg ggtttcgccc gatggcagcc gcgccctgat
     5401 gctggtgcgg ttgcgtgccg cgggctccga tatcgacggg gcgcagcggg ccctggcaac
     5461 ggttcagtcc gcgttcgaca aggccgcctc cgatcccgcg gatcaggcgg ccagactggt
     5521 gatgactgga cctccggtct tcgccgtcca ggcgcgagac cggattaaag ccgatgcgac
     5581 ccggctttcc gcgttggcga gcctgctcgt ggccacggca ttgctcgtgg tctatcggtc
     5641 gccgcgtgtc ctggcactcg ccttcgtgcc ggtggtcagt ggcgccgtga tcggaatcgc
     5701 cgcggtcggc ctggtattcc ggaccgtcca cggcatcacc ttgggcttcg gtgcaacgtt
     5761 gatcggcgag gcagtcgatt acgccgttta tctgttcacc cagacttccc ccaacagtcc
     5821 ggtccagccg acgatgggcc gcatctggcc aacgcttcgg ttgggtgtcc tgacctcgat
     5881 ctgcggcttc tccgccatgt tgctgtccag cttcgaaggg ttcgcgcagc tcggtttgtt
     5941 cacgatcacc ggcctcgcgg tcgcggtttg cgtgacgcga tgggtgctgc cagccctgat
     6001 cccggagcgg ttcgccgggg ttcggcagtc ccggattatg atgcggatag cgacctttgc
     6061 tgcgggcttg cgccgccttc gaatcggcgt cctggtgctg gcggccaccg cgaccggcgc
     6121 gcttgccatg agtaaaggcg cattttgggc gggcgacctc gccagcctca gcccggtgcc
     6181 cccggcggag cagcgtctgg accaatcgtt gcggcgggac atgcgggcgc cggatgccgc
     6241 ttacctgatc gtggtccggc gggctgacca ggaagcggca ctccaggccg ccgaacggct
     6301 gacgacttcg ctcgttccgc ttgtcgcaca gggcgcgctg tccggcatcg actcccccgc
     6361 gcggtacctg ccgagcctgg cggcgcagcg gtcgcggcag gctgtcctgc ccgatccggc
     6421 tattttgcag gtcgagctcg atgcggcgct cgatgacctg ccattccagc cgggcttgtt
     6481 cgcgccgttc attgccgcga tcggcaatgc gcgatccggg tcgccgatta cccgcacaga
     6541 actggatggc acgtcgcttt ccctgaagct cgactcgctg ttgctgcatc ggcaggacgg
     6601 ctgggtcgcc atgctgtcct tgcgcggcgt caccgcaccg gacgtcattg cgacggcagt
     6661 tgccggtgaa ccggacactg tctttgttga tctgaaggcc gagtcggatc gcctgctggg
     6721 ggtgtacctg cgggaaggga tcagtcttgc gggcctgggc gccgcggcga tcggtctgct
     6781 gctggcggtg agcctgcgat cgctgccgcg gctggccgcc gtcacggcac cgctggtggc
     6841 ttccgtcctg gtgacgctgg caattctccg cctgggcggg catgtgttgt cagtcttcaa
     6901 cctgttcgga atgcttctgg tggtcgccat cggctcgaac tattgcctgt tcttcgaccg
     6961 gcagcgcggt gatccggcgg ggatgccccg ggtgctggcg tcactgctgc tggctaacgc
     7021 ctgtaccgta gccggctttg gcgttctggc attgtcgcat acgccgg
//
LOCUS       DASWQN010000661         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_854847, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000661 DASWQN010000000
VERSION     DASWQN010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854847"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VGC82_11280"
     CDS             complement(<1..261)
                     /locus_tag="VGC82_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423894.1"
                     /translation="MGLGDTTSDETFDVVYLILSGTTTAARCPELLRGLVGLGFSTVV
                     AVPTPNASRVIAPRDLADVEGVRVVESYFDAAIRPRPPRGVVL"
     gene            complement(316..1791)
                     /locus_tag="VGC82_11285"
     CDS             complement(316..1791)
                     /locus_tag="VGC82_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0423895.1"
                     /translation="MDSPLPQEWDVEADVVVVGFGAAGVATAVTAHDLGAKVVILEKA
                     PEGQEGGNTRVAGQGYLNTSSVEQAAAYLTALCGPYTVPEAMVKVWAQEMGQNNAWLE
                     SLGGDPQEHQHPPVGIEFPDLPGSECVHKFHDGPTYGYSNTWKQFERLVKQRPIPILY
                     ETPGKELIQHAVTGEILGVRAWQGHKSVAVKASRAVVLTCGGFENNQEMIRNYLPGVP
                     YCYTSGTPYNEGDGITMAMSVGADLWHMNNFAGPSMALKAPEIRTSFSMQALHYSKET
                     PGGMIVVGPDAKRFTDEKYKTRHGKVPVNGRWFPLSTPCPMFLIFDHTLFSSGPLYDK
                     NPSHGWTQIIERYDWSENNSVELAKGWIKTAGSLVDLASIVGLDPAALVETVSRWNHF
                     CDANHDADFGRSLMLAPIVLGPFYAVELSPSMLNTQGGPRRNERGQIVRPNGKPIARL
                     YSAGELGSIYSYLYQGTGNIGECLAFGRISGRNAAAETPWA"
     gene            complement(1791..>2703)
                     /locus_tag="VGC82_11290"
     CDS             complement(1791..>2703)
                     /locus_tag="VGC82_11290"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEY0423896.1"
                     /translation="IGVRYTANGQQHEARANREVIVSAGSINSPQLLELSGIGQPDLL
                     QSLGIEVRHALKGVGENLRDHYSPRMKWTVPAALGLTYNDKGRGLGLVWQALKYALTT
                     KGLLGLPASPIRAYVRTRAGLDAPDAAISWIPFLAGANFKLAKNSGITAIMNILRSES
                     TGSIHVSSQSPNRPPAVRFNFLSAQLDREVTLEAMRITRRIMTAPAMQGIATDEIAPG
                     VNIQADDELLDWVRANAETTYHPVGTCKMGSDPMAVVDSELRVHGMEGLRVADASIMP
                     TLTSGNTNAPSIMIGEKASRLVLAAAA"
BASE COUNT          494 a          852 c          821 g          536 t
ORIGIN      
        1 cagcacgacc ccacgcggcg gccgcggacg gatggccgca tcgaagtatg attccaccac
       61 ccgcacaccc tcgacgtcag ccaggtcacg aggcgcgatc acccgcgaag cgttcggtgt
      121 cggcaccgcg acgacggtag aaaagcccag cccgaccaga ccgcgcaaaa gctctggaca
      181 gcgtgccgcg gtcgtcgtgc cgctgagaat tagatagacg acgtcgaagg tttcgtcact
      241 ggttgtgtca ccaagaccca tcgcgctgcc tccgtacccg gtctccagac ttgttccttc
      301 gaaatcggac aactgctacg cccaaggcgt ttcagcggcc gcgttccgcc ctgaaatgcg
      361 gccaaacgcc aagcactccc caatatttcc ggtgccctga tacaagtagc tatagatcga
      421 acccagttct ccggcgctgt agagcctggc gatgggctta ccgttgggcc ggacaatctg
      481 ccccctctca ttgcgtctcg gaccgccttg ggtgttgagc atcgacggcg agagttcaac
      541 cgcgtaaaac ggccccagaa cgatgggcgc cagcatcagg gaccggccga aatccgcgtc
      601 gtggtttgcg tcacagaaat ggttccaccg gctgacggtc tccaccagcg cggcgggatc
      661 cagcccaacg atcgaggcaa gatccacaag actgccggcg gtcttgatcc atcccttcgc
      721 cagctcgacg ctgttgttct cgctccaatc gtagcgctcg atgatctgcg tccaaccgtg
      781 actcgggttc ttgtcgtaca ggggcccaga ggaaaacagc gtatgatcga atatcaaaaa
      841 catcgggcac ggcgtggaca acggaaacca gcgtccattc accgggactt tcccatgccg
      901 ggtcttatac ttctcgtcgg tgaagcgttt ggcgtccggg cccacgacga tcatcccgcc
      961 cggcgtttcc ttactgtagt gtagcgcctg cattgaaaag gaggtccgga tctccggagc
     1021 cttcaacgcc atggaggggc cggcaaaatt gttcatgtgc caaagatcgg ctccgaccga
     1081 catcgccatc gtgatgccgt cgccttcatt gtacggcgtg ccggaagtgt agcagtaggg
     1141 gacaccagga aggtaattgc ggatcatctc ctggttgttt tcgaagcctc cacacgtcag
     1201 cacgactgcc ctgctggctt tgacggcgac tgatttatgc ccctgccacg ctcgcacccc
     1261 aaggatttcc ccagtgacgg cgtgctggat cagttctttc ccaggcgttt catacaggat
     1321 ggggataggg cgttgcttga ccaggcgctc gaactgcttc caggtgttgg agtagccata
     1381 ggtgggccca tcgtggaatt tgtgcacaca ctccgaaccg ggtaggtcgg gaaattcaat
     1441 gccgaccggc gggtgctgat gctcttgcgg atcgccccca agactttcaa gccatgcgtt
     1501 gttctggccc atctcctgcg cccatacctt caccatggct tccggaacgg tgtaaggtcc
     1561 gcacagggca gtgagatagg cggcggcttg ttcgaccgac gacgtattca ggtagccttg
     1621 gcccgcgacc cgcgtgttgc cgccttcctg accttccggc gccttctcca ggataaccac
     1681 cttggctccc agatcgtggg ccgtgacggc ggtcgccacg ccggctgctc cgaatccgac
     1741 gacaacgaca tcggcttcca cgtcccattc ttgcggcaag gggctgtcca ttacgctgcc
     1801 gccgcgagca ccaaccggga tgccttctcg ccgatcatga tggacggcgc gttggtgttg
     1861 cccgaagtga gcgtcggcat gatcgatgcg tcggcgacgc gcaacccctc catgccatgc
     1921 acgcggagtt cgctgtcaac caccgccatg gggtcggagc ccatcttgca ggtaccgacc
     1981 ggatggtagg tggtttcggc gttggccctg acccagtcca gcagttcgtc gtcggcctgg
     2041 atattgacgc ccggcgcaat ctcgtcggtg gcgatgccct gcatggcggg tgcggtcatg
     2101 atgcgccttg tgatgcgcat cgcttccagc gtgacttcgc ggtcgagctg ggcactgagg
     2161 aagttgaagc ggaccgctgg cggcctgttt ggcgactgcg aggacacatg aatactgccg
     2221 gtgctttccg aacgcaggat gttcatgatc gcggtgatgc cggagttctt cgcgagcttg
     2281 aagttcgcac cggccaggaa cgggatccag gagatcgcgg catccggcgc atccagcccc
     2341 gcccgggtgc gaacgtatgc ccggatcggc gaggccggca gcccaagcag ccccttggtg
     2401 gtcagcgcgt atttcaacgc ctgccaaacc aggccgaggc cgcgcccctt gtcgttgtag
     2461 gtcaggccca gtgcagccgg caccgtccat ttcatgcgcg gcgaataatg gtcgcgcagg
     2521 ttctcgccga cgcccttgag cgcgtggcgc acctcgatgc cgaggctttg cagcaggtcg
     2581 ggctggccga tgccggacaa ttccagaagc tgcggggaat tgatcgagcc ggcactgacg
     2641 atgacctctc ggttggcgcg ggcttcgtgt tgctgtccgt tggcggtata gcgcacgcca
     2701 atg
//
LOCUS       DASWQN010000662         2935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_854943, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000662 DASWQN010000000
VERSION     DASWQN010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854943"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1997
                     /locus_tag="VGC82_11295"
     CDS             <1..1997
                     /locus_tag="VGC82_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0423897.1"
                     /translation="RVRNHRALTALTLLYLPIGLFMTQASAAELTLQIPETVVTATRI
                     PTPPDQIAAGVSIVDRAAIEAHGYNSLPQALADIPGLHVSPAGGQGGQTSVFIRGSNS
                     NHVLVLRDGMPITDASDPTAAFNFGTDTLADVERIEVIRGPMAAVYGSGAIGGVINLI
                     SRRGTEPGIHWSGDLAGGYPALIRGSVTASGVQGPLDFALTAESQSQRGYDAVPQRQS
                     IYRGVPQGFRDRTLTLNLGYTPIEGTRLSLFLRANTAWFGFNTLGSPTFDNSNSNGEW
                     TSLLGRVGGATHLFDGKLESSVFVGQAQDDRRYLEPFAPADPNLASSDSRYHSYRTDV
                     QWNNTLHLDDLIALPALSASAMTFGYEYIGDRVKVRVNENSFGQSVAASMTDNAIYAG
                     LQTTVVQRLALTGQVRQDWVAGNSPTTWRIGAVYDLKEIATHLKVAYGTAYRAPSLFD
                     RFGVDTFGYVGNPALKPEQARGWEAGFTTDIAALNRADFASVGATYFDQRVRDLIVGV
                     FAPVDTAVNLGSAHVRGVETEATLRPARWLDLHATYTFLDTSASGQSASQGSQLLRRP
                     QNEASFDVTIRPVPDLRLLATLIYTGSAHDFLYDNNGNGIGNGVGQHGLIANAAANYT
                     VTPAVELYLNGWNILYSKFEPVNGYQTPGPTVLAGVRIRL"
     gene            complement(2019..2678)
                     /locus_tag="VGC82_11300"
     CDS             complement(2019..2678)
                     /locus_tag="VGC82_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0423898.1"
                     /translation="MEKLHLPHLVGAVAGRSEALIVALGSSSTAGVMASDSAHTYPAV
                     LQTALNRGLPSAHFAVINRGIGGQDAPEELARLDADVIAVRPQLVIWQVGANGAMRQA
                     DPSEFHRMVEEGVNRLLKAGSDVILMDNQRSPKLLAAVEHIVLEDSLGHVARETGVNL
                     FSRSHLMDAWSEEGSKPGLFTASDGLHHNDLGYYCVTQVLARQIIATVNASSSAVSAS
                     R"
BASE COUNT          539 a          929 c          925 g          542 t
ORIGIN      
        1 gtcgcgtgcg taatcatcgc gctttgacgg cattaaccct gctatacttg ccgatcgggc
       61 tgttcatgac gcaggccagc gctgccgaac tgacgctgca aatccccgaa acggtcgtga
      121 cggcgacccg catacccaca ccccccgatc agattgccgc cggcgtctcg atcgtcgatc
      181 gagcggcgat cgaggcgcac ggctacaaca gcctgcctca ggcgctggcc gatatccccg
      241 ggttgcatgt cagcccggcc ggcggacagg gcggccagac gagcgtgttt atccgcggca
      301 gcaactccaa ccatgttctg gtgctgcgcg acggcatgcc gataaccgac gcatcggatc
      361 ccacggctgc gttcaacttc ggcaccgata cgcttgccga cgtggagcgg atcgaggtca
      421 tccgcggtcc gatggcagcc gtttatggtt cgggggcgat cggtggcgtg atcaacttga
      481 tctcgcgccg gggtacggag ccgggcattc actggagcgg cgaccttgcg ggcggctatc
      541 ctgccctgat acggggcagc gtgaccgcca gcggcgtgca gggtccgctg gatttcgcgc
      601 tgacggcgga atcccagtcg cagcggggct atgacgccgt tccgcagcgc caaagcatct
      661 atcgcggggt tccgcagggc ttccgtgacc ggaccctgac gctgaatctg ggctatacgc
      721 cgatcgaagg caccaggttg tcgctgttcc tgcgggccaa caccgcctgg tttggcttca
      781 ataccctggg atcgcctacc ttcgacaatt ccaattccaa tggcgaatgg acatcgttgc
      841 taggacgggt cggcggcgcc acgcatttgt tcgacggcaa gctggaaagc agtgtcttcg
      901 tcgggcaggc gcaggacgac cggcggtatc tggagccgtt cgcgccggcc gatccaaacc
      961 tggcgtcgtc cgattcgcgg taccactcct atcgcaccga tgtgcagtgg aacaacacgc
     1021 tgcatctgga cgatctgatc gcgctgccgg cgctttccgc cagcgccatg accttcggtt
     1081 acgagtacat cggcgacagg gtcaaagtcc gggtgaacga gaactcattc ggccagtccg
     1141 tcgcggcgtc gatgaccgac aatgcgatct atgccggctt gcagacgacg gtcgtgcagc
     1201 gcctggccct gacgggtcag gtgcgccagg actgggtcgc cggcaactcc cccacgacat
     1261 ggcgcattgg cgcggtttac gatctgaagg aaatcgcgac gcatctcaag gtcgcctatg
     1321 ggacggccta ccgggcgcca tcgttgttcg acaggtttgg cgtggatacg ttcggctatg
     1381 tcggaaatcc ggcactgaaa cctgaacagg cacgcgggtg ggaagccggt ttcacgaccg
     1441 acatcgcggc tttgaaccgc gcagatttcg cctcggtcgg cgcgacctat ttcgatcagc
     1501 gcgtgcgcga cctgattgtg ggtgtgttcg cgccggttga taccgcggtc aatctcggct
     1561 ctgcccatgt gcgtggtgtc gagaccgagg cgacgctgcg cccagcccgt tggctcgacc
     1621 ttcatgccac ctacacattt ctcgatacgt ccgcgtccgg ccaatcggct tcccaaggct
     1681 cccagttgct ccggcggccg cagaacgagg cgtccttcga cgtgacgatc cggccggtgc
     1741 ccgacctgcg ccttcttgcg acgctgatct acaccgggtc tgcgcacgac ttcctgtacg
     1801 acaacaacgg gaacggcatc ggcaacggtg tcggccagca tggcttgatc gccaacgcgg
     1861 cggcgaacta cacggttacg ccggcggtcg agctgtatct caacggctgg aacatccttt
     1921 actcgaagtt cgagccggtg aacggatacc agacgcccgg cccaacggtg ttggccggcg
     1981 tcaggatcag actgtgacgc aggatggcag gaccatactc atcggctggc cgaaacggcc
     2041 gatgagctgg cattgaccgt ggcgatgatt tgacgcgcga gaacctgggt gacgcaatag
     2101 tagccaaggt cgttgtggtg caaaccgtcc gaggccgtga acagcccagg tttggagccc
     2161 tcctcggacc acgcgtccat caggtgactg cgggaaaaca gattcacgcc ggtctcgcgc
     2221 gcgacgtgac caagcgagtc ttccaggaca atgtgttcga ccgccgccag cagcttgggt
     2281 gagcgctggt tgtccatcag gatgacgtcg cttccggctt tcagcagacg attgacgccc
     2341 tcctcgacca tccggtggaa ttccgacgga tcggcctggc gcatggcgcc attcgcgccg
     2401 acctgccaga tcacgagttg cgggcgcacg gcgatgacgt cggcatccag acgagccagt
     2461 tcctcgggcg cgtcctggcc gccaatgccg cggttgatga cggcaaaatg cgcgctcggc
     2521 agtcccctgt tcaatgcggt ctgaagcacc gccggatacg tatgcgcact atcggatgcc
     2581 atgacgcctg ccgtcgagga ggagccaagg gcgacgatca atgcctccga ccgccccgcg
     2641 acggcgccga cgagatgcgg cagatgcaat ttctccatcg gcggatcgga tgggcaggtc
     2701 gcagccgcgc ggcatgtcag ggggacggaa agtccgatga cacaacaaaa aacgccgaaa
     2761 aaatgctttc ggttcgatgg tctcttcatc ccggactgat gccgcattgc ttacgggctg
     2821 tccagtacga aggacccttc tgtccaaacg tggtggcgtg cgatcggccg tgctatgggc
     2881 gcggccatcc acaacgtctt tgcaaccacg ggaaacccca gcacctgcca ccctg
//
LOCUS       DASWQN010000663         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_986227, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000663 DASWQN010000000
VERSION     DASWQN010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986227"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="VGC82_11305"
     CDS             <1..1367
                     /locus_tag="VGC82_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY0423899.1"
                     /translation="TSYITAAAIMTAPVGWMASRYGRKPLHIACIIGFTIASMLCGAA
                     QSLTQIVVFRLLQGMFSAALVPLAQTTLLDIYPPERRGFAMAIWGMGVMIGPIMGPTL
                     GGYLTENYDWRWVFYINLPFGLLAAAGLLIFLPNGREPTGGRFDWLGFTLLSTGIGAL
                     QLMLDRGQDLDWFSAREIVVEAILAGLGFYLFTVHLCSARQPLLRPNVFRDRNFLFGL
                     IMMFAVGTLLVSSLALMTPWLQVMSDYPVETAGLIMAPRGLGNFAAIMLSGRLVNRID
                     ARWLVATGLIMLCASFYVMTGWTPDVSQRDIILTIALQGGGLGLVFMPLQVLAFATLP
                     AALRTEGAALFSLLRNIGAAIGVSVTSTVLARNTQSLHEVIGASVTPFNRALAGIGSL
                     DPATHKGAAMLDKLISQQAEIIAYGNEYVLLICTTLPAVLLLLLMRRPAPASGPAVAA
                     EPVE"
BASE COUNT          238 a          495 c          514 g          337 t
ORIGIN      
        1 tgacatccta tatcaccgcc gcggcgatca tgactgcgcc ggtgggttgg atggccagcc
       61 gatacggtcg caagccactt catatcgctt gcatcatcgg cttcacgatc gcgtcgatgc
      121 tctgcggggc ggctcagtcg ctgacgcaga tcgtggtttt ccgtctgttg caaggcatgt
      181 tcagcgccgc actggtgccg ctggcgcaga ccacgctgct ggatatctac ccgccggagc
      241 ggcgtggctt cgccatggcg atctggggta tgggggtgat gatcggcccg atcatgggac
      301 cgaccttggg cggatacctg acggaaaact atgactggcg atgggttttc tacatcaacc
      361 tgccattcgg gcttttggcc gcggccggtc tgttgatatt cctgcccaac ggccgggagc
      421 ccaccggagg gcgtttcgac tggctgggat tcaccctgtt gtccaccggc atcggtgcct
      481 tgcagttgat gctggaccgc ggtcaggacc tggattggtt ctccgcgcgg gagatcgtgg
      541 tcgaggcgat cctcgccggc cttggcttct atctgttcac cgtgcatctg tgttccgccc
      601 gacaaccgct gttgcgcccg aacgtgttcc gtgaccggaa ttttttattc gggctcatca
      661 tgatgttcgc ggtggggacg ttgctggtct ccagcctggc gctgatgacg ccatggcttc
      721 aggtcatgag cgactatccg gtggaaaccg ccggcctgat catggccccg cgcgggcttg
      781 gcaattttgc cgccatcatg ctcagcggcc ggctggtaaa tcggatcgat gcccgttggc
      841 ttgtcgccac cggactgatt atgctgtgcg cctcgttcta cgtgatgacc gggtggacgc
      901 cggatgtttc gcagcgcgac attatcctga ccatcgccct gcagggcggc ggactgggtc
      961 tggtgttcat gccgctgcag gttcttgcgt tcgccacgct ccccgctgcg ctgcgcaccg
     1021 aaggcgccgc gctgttcagc ctgctgcgca atatcggcgc ggcgatcggc gtatcggtta
     1081 cctcgacggt cctggcacgc aatacgcaat cgctgcacga ggtgatcgga gcgagcgtca
     1141 caccttttaa ccgggcgctg gcgggcatcg ggtcgctcga tccggcgacg cacaaggggg
     1201 cggcgatgct ggacaagctg atcagccagc aggcggagat catagcctat ggcaacgagt
     1261 acgttctgct gatctgcacc accctgccgg cggttctgct gctgctgctg atgcgccgcc
     1321 ctgccccggc aagcggacca gccgtcgccg ctgaaccggt ggagtagcga gttggagcag
     1381 cgaccgcttg acaggcgccg cgccgtccca gcccggcttc ccccaatgtc gtgtcgaagg
     1441 tttccgtgac ctgtctggcg aacccgcccc gatgagcgat gtggagacag actggccgcc
     1501 gtaccagcgg ccggtcgcgg tgatccaacc gttgcccggc attggcgaca tggtgtggca
     1561 cctgccgcat atccgcgcga tcgc
//
LOCUS       DASWQN010000664         2941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_986530, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000664 DASWQN010000000
VERSION     DASWQN010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986530"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VGC82_11310"
     CDS             <1..825
                     /locus_tag="VGC82_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423900.1"
                     /translation="LWRRQEAGVTTLHEHVAQLLRQQLDAPNVPAEKLNAALRLLAKC
                     RATLIQNTVVQQCGSTVQGGPFQGMRFVEQSAEGCHVPKLLGCYESELHAHILAAAGR
                     DYQAVINIGAAEGYYAVGLARLMPDVRVFAYDTNAASHPVCRSLAERNGVADRVVVGG
                     LFDGEAFGRFAGQRTLVVCDIEGGETELLDPTRHPALRAMDLIVELHDSPQAKPSLLI
                     PQRFAATHAVTIVRHRGQAIELPPMFDALGHLDQLLAIWEWRSEPTPWAVMQVRTA"
     gene            795..2522
                     /locus_tag="VGC82_11315"
     CDS             795..2522
                     /locus_tag="VGC82_11315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0423901.1"
                     /translation="MGGDAGSDSLTDMNDIANVAIRFEPDAYLLSGPKLMGRQAAGNA
                     FLRAAVAGRKGQALWAFTPNRNSAEAFAALVRTLDGDAEARWLPADRLDLLGQIGTLY
                     LPGPSLGEAARLRLRAGTAAYSLCGVTHTTASHAAMDDITGLLTAPVMPWDALICTSA
                     AVARTVKLLLEAEIAALRWRFGPSIAITLPRLPVIPLGVHCDDFVFAPRERENARTAL
                     GIAADEVVALFVGRLSFHAKAHPHAMYAGLQAAAQRTGKKLVLLQSGWFATETIEQAF
                     RDGAARTCPDVRALFNDGKDPDSRRLAWAAAALFVSLSDNIQETFGLTPIEAMAAGLP
                     VVVTDWDGYKDTVRDAVDGFRIPTWMPPPDGEPFARGYEAGIDTYDLYAGLTCQTVSV
                     DMQVLIDRLSQLVSDRRLRQRIGEAGRRRAREVFDWAVVYRQYQALWAELGQLRQAAG
                     SDPGQQAQIAAAPRAAASRMDPFRGFAHYPTTTIQPRTHVSLRPGADGTAYAELAGDA
                     LFNYAGKVLPAQPVVETLLALLTPGELMIQDLAGRAGLDLGTAVLGISVLAKMGLLRL
                     RAPDVSGLA"
     gene            2687..>2941
                     /locus_tag="VGC82_11320"
     CDS             2687..>2941
                     /locus_tag="VGC82_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="cytochrome c-type biogenesis protein; required for
                     the transfer of heme to apocytochrome c; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HEY0423902.1"
                     /translation="MITELGHFALILALFVAIIQSTIPLVGAARRHLGWMAVGRSCAL
                     TGFALTALAMLALMHAYVTSDFTVINVIENSHTDKPLLYRI"
BASE COUNT          490 a          983 c          950 g          518 t
ORIGIN      
        1 ctgtggcgaa ggcaggaagc gggcgtgacc accctgcacg agcacgtggc acaactcctg
       61 cgccagcaac tggacgcgcc gaacgtaccc gcggagaagc tcaacgccgc gctgcgcctg
      121 ctcgccaagt gtcgtgcgac cttgatccag aacacggtcg ttcaacaatg cggttcaacg
      181 gtccagggcg gtccgttcca ggggatgcgg ttcgtcgagc agtccgctga agggtgccat
      241 gttccgaagc tgctgggctg ctacgaatcg gaacttcacg cccacatcct ggcggccgcc
      301 ggacgcgact accaggccgt catcaacatc ggcgcggcgg aaggatatta cgccgtcggc
      361 ctcgcacgcc tgatgcccga tgtccgcgtc tttgcctacg acaccaacgc cgcttcgcat
      421 ccggtttgcc ggtccctcgc ggaacgtaac ggcgtggccg atcgtgtcgt cgtcggaggc
      481 ctgttcgacg gcgaggcatt cgggcgcttt gccggacaaa gaacactcgt ggtgtgcgac
      541 atcgaaggcg gcgagaccga actgctcgat cccacgcgcc atcctgcgct gcgggcgatg
      601 gacctgatcg tcgagctgca cgacagccca caagcgaaac caagcctgct gatcccgcaa
      661 cggttcgctg ccacacatgc cgtcacgatc gtccgccatc gcggacaagc aatcgagctg
      721 ccgccgatgt tcgatgcgct gggccatctc gaccagcttc tggcgatttg ggagtggcgt
      781 tcggaaccga cgccgtgggc ggtgatgcag gttcggacag cctgacggac atgaacgaca
      841 tcgccaacgt ggccatccgg ttcgagccgg acgcctacct tctgtccggt cccaagttga
      901 tgggccgtca ggcggcggga aacgccttcc tgcgtgcggc ggtcgccggc cgaaagggtc
      961 aggcactatg ggcctttacc ccgaacagga attccgccga ggccttcgcc gccctggtgc
     1021 gcacattaga cggcgacgcc gaggcacgct ggttgccagc cgaccgcctg gacctgctgg
     1081 gccagatcgg cacgctctat ctccccggac caagcctggg cgaagcggcg cgcctgcgcc
     1141 tgcgagccgg gaccgcggcc tacagcctgt gcggcgtcac ccacaccacc gcctcgcatg
     1201 cggcaatgga cgacatcacc ggcctgctca ccgcgccggt catgccatgg gacgcgctga
     1261 tctgcacctc ggcggccgtg gcacgcacgg taaagttgct tctggaagcc gagatcgccg
     1321 ctctgcgctg gcgcttcggg ccgtcgatcg cgatcacgct gccgcgtctt cccgtcattc
     1381 cgctcggtgt gcactgcgac gacttcgtgt tcgccccgcg ggagcgggaa aatgcgcgaa
     1441 ccgcactcgg gatcgccgcg gacgaggtgg tggcgctgtt cgtcgggcgg ctgtccttcc
     1501 atgccaaggc gcatccgcat gcgatgtatg ccggtttgca ggcagcggcg cagcgtactg
     1561 gcaagaaact ggtgctgctt cagtccggct ggtttgccac cgagaccatc gaacaagcgt
     1621 tccgcgacgg tgccgcgcgc acctgccccg acgtgcgggc gcttttcaac gacggcaagg
     1681 atccggacag ccgccgcctg gcctgggcag cggcggctct ttttgtttca ctttccgaca
     1741 atattcagga aacctttggt cttacgccga tcgaggcaat ggccgcgggg ctgccggtcg
     1801 tggtgaccga ctgggacggc tacaaggaca cggtgcgcga cgcggttgac ggtttccgca
     1861 tccccacatg gatgccgccg cccgacggcg agccgttcgc tcgcggctac gaagccggca
     1921 tcgacacata cgacctttat gccgggctga cctgtcaaac ggtgtcggtg gacatgcagg
     1981 tcctgatcga ccgcctcagc caactggtca gcgatcgccg acttcgtcaa cgaatcggag
     2041 aagccggccg ccgccgggcc cgggaagtgt tcgactgggc ggtggtgtac cggcagtacc
     2101 aggcgctatg ggccgaactg ggccagctcc gccaggcggc gggcagcgat cctgggcagc
     2161 aggcgcagat tgcagcggcc ccccgagccg cggcaagccg gatggacccg ttccgcggct
     2221 tcgcccatta tccgaccacg acgattcagc cgcgaacgca tgtttcgctg cgacccgggg
     2281 ccgacggtac ggcttacgcc gaactcgccg gcgacgcgct gttcaactat gcgggcaagg
     2341 tcctgccggc ccagcctgtt gtcgagacgc tgctggcgct gctgacgccg ggcgaactca
     2401 tgatccagga tcttgccggg cgtgccggat tggacctggg aacggccgtg ctgggcatct
     2461 cggttctggc caaaatgggg ctgctgcggc ttcgcgctcc ggatgtctcc ggattggcat
     2521 aacctcgacc tgccgctccg acgcatcgag agaaaccgtg ctgtattgcg tccttgtccc
     2581 gggaacgctg ggtggtatat cggccttgcc ggtcgagatc gatattccgt tcggggaagc
     2641 ggctccgctc gcgttgcctg ccaaaggaca tatagggtca gcacccatga tcactgaact
     2701 cggccatttc gcgctgatcc tcgcgctctt tgtcgccatc atccaatcga cgatcccgct
     2761 ggtcggtgcc gctcggcggc atctcggctg gatggcggtg ggacgatcct gtgcgctaac
     2821 cggcttcgcg ctgaccgccc tggcgatgct ggcactgatg cacgcgtacg tcacctcgga
     2881 cttcacggtg atcaacgtca tcgagaacag ccacaccgac aagccgctgc tgtaccggat
     2941 c
//
LOCUS       DASWQN010000665         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_327627, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000665 DASWQN010000000
VERSION     DASWQN010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327627"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..622
                     /locus_tag="VGC82_11325"
     CDS             143..622
                     /locus_tag="VGC82_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0423903.1"
                     /translation="MTEPPDIRDHLGRFLDWEEAHVGFDKAVSGIPHDKRGAVPAGFE
                     HSPWQLLEHIRLAQDDIRDFCVKADYVHTMKWPDDYWPKNPAPPNDKAWADSIAGYRR
                     TLEELKQMARDAKDLTAKVPTGKPTQTYLRAILMTGDHTAYHVGQLVAVRRALGAWK"
     gene            complement(731..>1777)
                     /locus_tag="VGC82_11330"
     CDS             complement(731..>1777)
                     /locus_tag="VGC82_11330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0423904.1"
                     /translation="LEVEMIQNAQVRRSRLETTLASMDSLGQHLAGIPGRKNLVWISG
                     GISMLSVTGAMGMGPNGSIQSFEDRVKQTSQKLAQQGVVLYMVDAKGLVLPSPMTMNS
                     SVPGTPPAQGRGRFEAQQQAESISADPRAAMELMASITGGRYLRNTNDLMEGFRKAAV
                     DLAGSYTLGFYVAEELDSKWHSLKASVKRPGVSLQHRQGYLADAVAAKPAGWPNDLAM
                     AVASNPIGSSAVRLTASCALTSDPEPGTLQVGLRIEPWSLRFAENGGFQQTQIQIIFA
                     ERSANGRTRLTTDTPTVKIPDQSWETAQREGLRYARRLKPARDAVSLRVIVRDMATGR
                     YGTLDVPLKALPVR"
BASE COUNT          301 a          598 c          567 g          311 t
ORIGIN      
        1 cggaaggtgc ggagatccac ttccgcaaca tccggttgat ggagttactg cccggtccgg
       61 cgtgatcttc gcgacgatgc cgccaccggc tcgcgggcac gtcacactgg aatcctcggc
      121 tgccgctcag gagtcgatcg aaatgaccga accacccgac atccgcgatc acctcggccg
      181 cttcctcgac tgggaagaag cgcacgtcgg attcgacaag gccgttagtg gtattcctca
      241 tgacaagcgc ggcgccgtcc ccgccgggtt cgagcactcg ccatggcaac tgctcgagca
      301 catccggctg gcgcaggatg acatccgcga cttctgcgtc aaggcggact acgtgcatac
      361 gatgaaatgg ccggacgact actggcccaa gaacccggcg ccgccgaacg acaaagcgtg
      421 ggccgacagc atcgcgggct accggcggac cctggaggag ctgaaacaga tggctcgtga
      481 cgcgaaggat ctgaccgcga aggtcccgac cggcaagccg acccagacct acctgcgcgc
      541 aatcctgatg accggggatc acaccgccta tcacgtcggc cagctggtgg cggtccgccg
      601 tgcgctgggt gcgtggaagt agcaggcggt tcgagcggac gtcacacgga tcatttttgc
      661 cacggaacac acggatcatc tttatccggg tactccgtgc aatccgtggc cagctacctg
      721 tgcgtgaaca ctagcggacc gggagcgcct tcagcggcac gtcgagggtt ccgtatcggc
      781 ccgtggccat gtcgcgcacg atgacgcgca gcgacaccgc atcgcgcgcg ggcttcaagc
      841 ggcgcgcgta acgaagccct tcccgttgcg ccgtctccca gctctggtcg gggatcttca
      901 cggtcggcgt gtccgtcgtc aggcgcgtgc ggccgttcgc ggagcgctcg gcgaaaatga
      961 tctggatctg cgtctgctgg aagccgccgt tttcggcaaa gcgaagcgac cacggttcga
     1021 tgcgcaggcc gacctgcagc gttccgggct cagggtcgga cgtcagggcg cacgaggctg
     1081 tcaatcgcac cgccgaggag ccgatcggat tggacgccac cgccatcgcc aggtcattcg
     1141 gccagccggc cggcttcgcg gcgaccgcgt cggcgagata accctggcga tgctggaggc
     1201 tcacgccggg gcgcttcacg ctcgccttga ggctgtgcca tttgctgtcg agttcttccg
     1261 ccacgtagaa gcccagcgtg tagctgcccg ccagatccac cgccgccttc ctgaatccct
     1321 ccatcaggtc gttggtgttg cgaaggtagc gcccgccggt gatcgacgcc atcaactcca
     1381 tcgcggcacg cgggtcggcg ctgatgcttt cggcctgttg ctgggcctcg aaccgcccgc
     1441 gcccttgcgc cggcggcgtg ccaggcacgc tcgaattcat cgtcatcggt gagggaagca
     1501 cgagtccctt cgcatcgacc atgtagagca cgacgccctg ctgcgcgagt ttctgcgagg
     1561 tctgcttcac ccggtcttcg aagctctgga tgctgccgtt cgggcccatg cccatggcgc
     1621 cggtcaccga cagcatcgaa atgccgccgc tgatccacac caggttctta cggccgggga
     1681 ttcccgccag gtgctgcccg agactgtcca tcgacgccag cgtggtttcg aggcgggacc
     1741 ggcgcacctg cgcgttctgg atcatctcca cttcgag
//
LOCUS       DASWQN010000666         3254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2306407, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000666 DASWQN010000000
VERSION     DASWQN010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2306407"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VGC82_11335"
     CDS             complement(<1..618)
                     /locus_tag="VGC82_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility-associated protein"
                     /protein_id="HEY0423905.1"
                     /translation="MLTIGGLVLLFGCVLGSYLASGGSMAPLLEAVPFEMWTIGGAAM
                     ATTLMSNSIHDIKHMLGAFGKMMKGAAFRKADYVELLSLLYYLVRLASTKGAMALEAH
                     IEKPAESAAFQKFPKIIANHHASTVICDYLRMVGMNADDPNQIEGVMARELKKTLVEE
                     LHPSHIMQSMADALPALGIVAAVLGVIKTMSHIDQPPAVLGEMIGG"
     gene            complement(629..1705)
                     /locus_tag="VGC82_11340"
     CDS             complement(629..1705)
                     /locus_tag="VGC82_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEY0423906.1"
                     /translation="MAVKGDKKGSKGSVIIKRYEIVEGGHHGGAWKVAYADFVTAMMA
                     FFLLMWLLNATTEDQRKGLADYFSPNNLMSHASSGTGQPFGGHTAFDEGALVSDRGSV
                     QITTGKRPVIERTEDGDDPVFSEFHRAQAGEGVGRGPMRDDQDADEEDKPMGQKSDRP
                     APGPGGASELERQGQPEAKPVTPAELAAEKARQEKAAFEQAAEQIKAAVRADPALAEL
                     SKQLAIDMTPEGLRIQILDEVKLPMFPSGSASPNERARLLMQKVVPVLMKLRQPLSIA
                     GHTDAAPFPGPDRTNWELSAERANATRRLLVEGGLPEARIKSVIGNADRNPLLPADPL
                     AAANRRIAILVLREAPVELPRSKH"
     gene            complement(1790..3097)
                     /locus_tag="VGC82_11345"
     CDS             complement(1790..3097)
                     /locus_tag="VGC82_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423907.1"
                     /translation="MPSAATEDKVIETVVMRRVFWRLMPFLLAAYLICYIDRVNVGFA
                     SLQMNKAVGIDPKTYGLGAGIFFIGYFLLEVPSNMGLEKFGASKWIARIMISWGAISG
                     AFALIGGPTSFLILRFLLGAAEAGFFPGVILFLTYWFPSEYRAKIVGIFMVAIPVAGL
                     TGSPISGAILGMDGVGGLGGWQWVFILEAMPAILLGFVSIFWLTDRPEHATWLTMTEK
                     TWLANKLLAERQRVSKVKQLSTWKVLFNKHVLIMALVYSGAAGASSAMALWQPQVIKS
                     FGLSNLETGFLNAIPYAISAVLMILWGRSSDRSGERVWHNALPLGWMGLALAATFFAH
                     NLWALLPLLTLVLAGTYCSKGPFWALCSEWLAAPVAAVGLAQINALGNLSGFFCNYLI
                     GWIKDVTGSFPLALMPIAALAAAGTIAVLVIGRDQPRTVAVRQ"
BASE COUNT          633 a         1059 c          959 g          603 t
ORIGIN      
        1 gccgccgatc atctcgccca acaccgccgg gggctgatcg atatgcgaca tcgtcttgat
       61 cacgcccaga accgccgcga caatccccag cgccggcagc gcgtcggcca tgctctgcat
      121 gatgtgcgag ggatggagtt cctcgaccag cgtcttcttg agctcccgag ccataacgcc
      181 ttcgatttgg tttgggtcgt cggcgttcat cccgaccatg cgcaggtaat cgcaaatgac
      241 cgtgctggca tgatgattgg cgatgatttt ggggaatttc tgaaacgcgg cgctttcggc
      301 cggtttttcg atgtgcgcct ccagcgccat cgcgcccttg gtgcttgcga gccgaaccag
      361 gtagtacaac aggctcagca gctcgacata gtccgcctta cggaatgcgg cgcccttcat
      421 cattttgccg aacgcgccca gcatgtgctt gatgtcgtgt atgctgttcg acatcagcgt
      481 cgtagccatg gccgcaccgc cgatcgtcca catttcgaac ggaacggcct cgagcagtgg
      541 ggccatgctg ccgccggacg cgagatagct tcccagcacg caaccaaaca gcagaaccag
      601 gccgccgatc gtcagcatcg ctcagatcct agtgtttgct acggggaagc tcgacaggag
      661 cctcccgcag cacgaggatg gcgatgcggc ggttcgccgc ggccagggga tcggccggca
      721 gcagtggatt gcgatcggca ttcccgatta ccgacttgat cctcgcctcc ggcaatccgc
      781 cctcgaccag caggcgccgt gtggcgttcg cccgttcggc cgacagctcc cagttcgtgc
      841 ggtccggccc ggggaacggc gcggcatcgg tatggccggc gatggagagc ggttggcgca
      901 gcttcatcag caccggcacg accttctgca tcagcagccg agcccgttcg ttgggcgagg
      961 ccgaccccga gggaaacatc ggcagcttga cctcgtcgag gatctggatg cgcagccctt
     1021 ccggggtcat gtcgatcgcc agttgtttgg acagttccgc cagtgccgga tcggcgcgga
     1081 cggccgcctt gatctgctcc gccgcctgct cgaacgccgc tttttcctgg cgcgcttttt
     1141 cggctgctaa ttcagccggc gtgacgggct ttgcttcggg ttgtccttgg cgctcaagct
     1201 cggacgcgcc gccgggaccg ggtgccgggc gatcgctttt ttggcccatg ggcttgtctt
     1261 cttcgtcggc gtcctgatcg tccctcatgg gaccgcggcc gacaccttcg ccagcctgcg
     1321 cgcgatggaa ttcactgaac acggggtcgt cgccgtcctc ggtccgttcg atgaccggcc
     1381 gcttgccggt ggtgatctgc acgctgcctc gatcggacac caatgcgccc tcgtcgaacg
     1441 cagtgtgacc gccgaatggc tggccggtgc ccgaggatgc gtgcgacatc aggttgttgg
     1501 ggctgaaata gtccgccaag cccttccgct ggtcctctgt cgtggcgttc agcagccaca
     1561 tcagcaggaa gaaggccatc attgcggtca cgaagtcggc gtaagcgacc ttccaggcac
     1621 caccgtgatg gccgccttcg acgatctcat acctcttgat gatgacgctt cccttggacc
     1681 ccttcttatc gcccttgacc gccatgtcac cctccagagg aggtgcaaca tgacggccaa
     1741 acgttaaccg agtgctaacc tggcagaaga aaatcctgtg cccggcttct tactgccgca
     1801 cggcaaccgt cctgggttga tcccgtccga tcaccagcac cgcaatcgtt cctgccgccg
     1861 ccagtgcggc gatcggcatc agcgccaatg ggaagctgcc ggtcacgtcc ttgatccagc
     1921 cgatcaggta gttgcagaaa aagccggaca aattgcccag cgcattgatc tgagccaacc
     1981 ccaccgcggc caccggtgcg gccagccatt ccgagcacaa cgcccaaaac ggacccttgc
     2041 tgcaataggt gccggccagg accagcgtca gcagcggcaa caacgcccac aggttatggg
     2101 cgaagaacgt cgccgccaac gccagcccca tccagcccaa cggcagtgcg ttgtgccaca
     2161 cccgctcacc gctgcgatcc gacgaccggc cccaaaggat catgagcaca gcggagatgg
     2221 cgtaagggat cgcgttgagg aatccggttt caagattgct caagccaaac gacttgatga
     2281 cctgaggttg ccaaagggcc atcgccgaac tggcacccgc ggcaccagaa tagaccagcg
     2341 ccatgatcag gacgtgcttg ttgaacagca ccttccaggt ggacagttgc ttgactttgg
     2401 atacgcgctg gcgttccgcc agcagcttgt tcgccagcca tgttttttcc gtcatggtga
     2461 gccaggttgc atgctccggc ctgtcggtca gccagaaaat gctgacgaaa ccaagcagga
     2521 tcgccggcat ggcttccagg atgaagaccc actgccagcc gccaagaccg ccgaccccgt
     2581 ccatgccgag gatggcgccg gaaataggcg atccggtcag ccctgcgacc gggatcgcca
     2641 ccatgaagat gccgacgatt ttggcgcggt attccgatgg gaaccagtat gtcaggaaca
     2701 ggatcacgcc ggggaaaaaa cccgcttccg cggcgcccag caggaagcgc aggatcagga
     2761 agctggtcgg accgccaatc aacgcgaagg cgcccgagat ggcgccccag gagatcatga
     2821 tgcgcgcgat ccacttgctg gcgccgaatt tctccagacc catgttgctc ggcacttcca
     2881 gcaggaaata accgataaag aagatgccgg cgcccagacc ataggtcttc gggtcgatgc
     2941 cgaccgcctt gttcatctgc agggacgcga agccgacgtt tacgcggtcg atgtagcaga
     3001 tcagataggc agccagcagg aacggcatca gccgccagaa cacccgtcgc atcacgacgg
     3061 tctcgatgac cttgtcctca gtcgcagccg aaggcatctc tttctcccaa atcacaacga
     3121 acgccagcct gttcccacaa cgctgaacac ggcagcgcta aggcaagaga cgaccccaaa
     3181 atcgctgcaa tgccatctgt tgccggcttc gacaaggcgc ttttccggca aatcgccagg
     3241 gatggtgtct gccg
//
LOCUS       DASWQN010000667         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_459450, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000667 DASWQN010000000
VERSION     DASWQN010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459450"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3015)
                     /locus_tag="VGC82_11350"
     CDS             complement(<1..>3015)
                     /locus_tag="VGC82_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY0423908.1"
                     /translation="VLDGTRLEDFDVETSTKRQLKGNIYLAKVVRVEPSLQAAFVEYG
                     GNRHGFLAFSEIHPDYYQIPIADRQRLIEMQAEEARREEEEEDLEMALGQASSAPDPE
                     PFQPSGVPPGQVPLAQPTPEPWALAAFPRMLAAADPVAEIRPTVATRLTEPEAWAKSE
                     TPRLLAAPADVPILEANALEADALGPNAVEANASGAIPLEIATQRVYEDDSDGDDSES
                     YDSNGDHVENLPPPETVGGGDQETGDDQRERRMPPRFLRNYKIQEVIRRRQILLIQVV
                     KEERGTKGAALTTYLSLAGRFGVLMPNSPRGGGISRKITSATDRRRLKEVMAELDIPR
                     GMGMIVRTAGANRPKPEIKRDCEYLLRLWDDIREHTMKSVAPALIYEEASLIKRAIRD
                     VYSRDIDEILVDGEEGWRAAHDFMRMLMPSHAKKVQLWRDNGQPLFARSQVEAQLDGM
                     LMPTVQLRSGGYLVINQAEALVAIDVNSGRSTRERNVEETALRTNLEAADEVARQLRL
                     RDLAGLIVIDFIDMESKRHNAMVERRIKEALKNDRARIQVGHISHFGLLEMSRQRLRP
                     SLAETSFIPCPHCGGTGHVRSTENAAIHVLRGIEDEGGKRRSAEIIVHVSTPVALYLL
                     NHKRERLREIELRYVMRVIIAADDAQIAPQFRIEKVRTRSPDELPVAMTAERSLPPPV
                     EDVEDDDEVEAGPVGAEDEDDDQDSDNQDNDSGEDTAEERAADAASGGGADEAERRKK
                     RRRRRRRGGRRDDQQPGAATGSTEEPAEGVAEVEVRGSEDGSESAAPAAVTPTLADSP
                     LVAGPAIAADEAQTEPVVAEATVEATENPPAPDDDPANRRRGRRGGRRRRRDNEGELS
                     PFAVPGADQPDLQPAYAGPTPADPFGGRAFDIFDVMDQAERAAEAKPASRTTPAVEIV
                     NDAAPEPQAVSEPEGAPQPEAASTPEAIPEPPVPPESEAAPGPQSVEPESVEPESVEP
                     ESVGPGLRAASAESGSASVEATENPPAPDDDPA"
BASE COUNT          446 a         1015 c          947 g          607 t
ORIGIN      
        1 agccggatcg tcatcaggcg ccggcggatt ttccgtcgct tcaaccgacg cgctgccgga
       61 ttccgcactt gccgcccgga gaccaggccc caccgactct ggctccaccg actctggctc
      121 caccgactct ggctccaccg attgagggcc cggcgccgct tcggattccg gcggaaccgg
      181 aggctctggt atcgcttcgg gcgtactcgc tgcctcgggt tgcggcgcgc cttccggttc
      241 gcttaccgcc tggggttccg gtgcggcgtc gttgacgatc tccacggctg gggttgtgcg
      301 ggatgcgggt ttcgcttcgg ccgctcgttc cgcctggtcc atcacatcaa agatgtcaaa
      361 cgcgcgcccg ccgaacggat cggccggagt cggtccggca taggccggtt gcagatcagg
      421 ctgatcggca cccggcacgg cgaacggcga caattcgccc tcgttgtcgc gccgccgccg
      481 ccgtccgccg cgccgcccac gccgtcggtt agccggatcg tcatcaggcg ccggcggatt
      541 ttccgtcgct tcaaccgttg cttcggcaac caccggttcg gtttgggctt cgtccgccgc
      601 aatggccgga ccggcgacca ggggagagtc tgccagcgtg ggagtcacgg cagctggagc
      661 agccgattcc gatccatctt cggaaccacg aacttccact tcagcgacgc cttccgcggg
      721 ttcctcggtt gatccggtgg ccgcacccgg ctgctgatca tcccgccggc cgccgcgccg
      781 ccgccgccgc cgccgtttct tgcgccgttc ggcctcgtcg gcaccgccgc cggaagccgc
      841 atcggcggcc cgctcctccg cggtgtcttc cccgctgtca ttatcctgat tgtcactatc
      901 ctgatcgtcg tcctcgtctt cggcaccgac cggaccagcc tcgacttcgt cgtcatcctc
      961 gacgtcttcg accggaggcg gcagggagcg ctccgcagtc atcgccacag gtagttcgtc
     1021 gggcgaccgc gtccggactt tctcgatgcg gaattgcggc gcgatctgcg catcgtcagc
     1081 ggcgatgatg acccgcatga cgtagcggag ctcgatttcg cgcagccgct cgcgcttgtg
     1141 gttcagcaaa tagagcgcca ccggcgtgga gacatggacg atgatctcgg ccgaacggcg
     1201 cttgccgcct tcatcctcaa tgccgcgcag cacatggatc gccgcatttt ccgtgctgcg
     1261 gacatggccg gttccgccgc aatgcggaca cgggatgaag ctggtctccg cgagcgaggg
     1321 acgcagccgc tggcggctca tttccagcaa cccaaaatgg ctgatatggc cgacctggat
     1381 gcgggcgcga tcgttcttca gcgcctcctt gatccgacgc tcgaccatgg cgttgtggcg
     1441 cttcgactcc atgtcgatga agtcgatgac gatcaggccg gccaggtcgc gcagacgcaa
     1501 ctgccgcgcg acttcatcgg ccgcctcaag gttggtgcga agcgcggttt cctcgacatt
     1561 gcgttcacgc gtggagcggc cggagttgac gtcgatcgcg accagcgcct cggcctggtt
     1621 gatcaccagg tagccgccgg agcgaagctg caccgtcggc atcagcatgc cgtcgagttg
     1681 tgcctcgacc tggctgcggg cgaacaaagg ctgcccgtta tcccgccaaa gctgtacttt
     1741 cttcgcgtga cttggcatca gcatgcgcat gaagtcgtgc gccgcgcgcc agccttcctc
     1801 gccatcgacc aggatctcgt cgatgtcgcg cgaataaacg tcgcggatcg cccgcttgat
     1861 caggctggcc tcttcgtaga tcagcgccgg ggccaccgac ttcatggtat gctcgcggat
     1921 gtcgtcccac agccgcagca ggtactcgca gtcgcgcttg atctccggct tcggccggtt
     1981 ggcgccggcc gtgcgcacga tcatgcccat gccgcgcggg atatccagtt ccgccatcac
     2041 ctctttcagg cgccggcggt cggtcgcgga ggtgattttg cgggaaatcc cgccaccgcg
     2101 cggggagttc ggcatcagca cgccaaaccg gccggcaagc gacaggtatg tggtcagcgc
     2161 cgcgcccttg gtgccacgct cctccttgac gacctggatc agcaggatct gccggcggcg
     2221 aatgacttcc tggatcttgt agttgcgcag gaagcgtggc ggcatacggc gctcgcgctg
     2281 gtcgtcgcca gtctcctggt cgccgccgcc tacggtctcc ggaggcggca ggttttcgac
     2341 gtggtcgccg ttggagtcat aggactcgct gtcgtcgcca tccgaatcgt cctcgtagac
     2401 gcgctgtgtc gcgatctcga gcggaattgc tcccgaggca tttgcttcca cggcatttgg
     2461 ccccagggca tctgcctcca aggcatttgc ttccaagatt ggcacatcag ccggcgctgc
     2521 cagaagcctc ggcgtttccg acttcgccca ggcttccggt tcggttaagc gcgttgcaac
     2581 cgtcggccga atttccgcga cggggtcagc ggcggcaagc atccttggga atgcggcaag
     2641 cgcccatggc tccggcgtgg gctgggccaa tgggacttga cctggcggaa ctccacttgg
     2701 ttggaagggt tcaggatccg gggcggacga tgcctgtcca agcgccatct ccaggtcttc
     2761 ttcttcctcc tcgcgacgag cttcctcggc ctgcatctcg atcaggcgct gccggtcggc
     2821 gatgggaatc tgatagtagt cggggtggat ttcgctgaac gccaagaacc cgtggcgatt
     2881 gccgccgtat tcgacaaatg ccgcctgcag gctaggttct acccggacga cttttgcgag
     2941 gtagatgttt cccttaagtt gtcgtttcgt cgacgtctca acgtcgaaat cttcaagtcg
     3001 ggtaccgtcc agcac
//
LOCUS       DASWQN010000668         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1251439, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000668 DASWQN010000000
VERSION     DASWQN010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251439"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VGC82_11355"
     CDS             <1..606
                     /locus_tag="VGC82_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY0423909.1"
                     /translation="MGGGGGSTVTAGGLLLRGEPDGNFVVLNAKSGEVLWKFQTGFGA
                     DAPPAVYEVDGQQYIAIVTGGNSLQGSATGDALWVFGMNGQLSPLPPPQPPSRIAGPL
                     GPIADGTSTIKIGDNNVEYAYWPARTRVKAGTAVTFTNVGDIMHTATAATAAGSVADK
                     WDTGALAKGESKSISFTELGTYYYICTPHPWMYGQVIVEAP"
     gene            734..2059
                     /locus_tag="VGC82_11360"
     CDS             734..2059
                     /locus_tag="VGC82_11360"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0423910.1"
                     /translation="MSRLQALENIRVVDFSWVRAGPWATRWLGALGAEIIKIEWPESE
                     RGRLPSSTTPQHLEVNLNTSGNFNDTNVNKKSVSLNVRSPKGLEIVKRLIAVSDIVIE
                     NFSSRVLRNWGLGYDELCRIKPDIVYVSMSGYGHTGRNHHYTTFGPVAQAVSGLTYLS
                     GMPDRPPAGWGWSYMDDTGGMYGAMCALTGLYHRNMTGHGQHIDQAQMVSSVPLNGPA
                     LLDFTVNGRGSRRVGYPPGNRAHWPGTPLVNNYRGPTVAPHNAFRTQPGGYNDWCVIV
                     CHSDEDWQRLVQVMGAPAWAASAKFVTAAGRLQHQAELDEQIEAWSMTLDKYELTERC
                     QEAGVRALPVQSAEDRVEHDPQLRHRQMYLPIEHPALGTHKVQNAPFQLSETPASNRL
                     PSPLIGQHTREIVEGLLGYSHEEVRAGFADGTFWPTQRARFPYMDEMLQ"
     gene            2056..>3015
                     /locus_tag="VGC82_11365"
     CDS             2056..>3015
                     /locus_tag="VGC82_11365"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0423911.1"
                     /translation="MNEALGNEALGPLAGLRILELADEKGQFCGKLLGDLGADVVKIE
                     PPSGERCRHIGPFLDDIPHPERSLSFWYYNTSKRGITLNLETADGQTLFGRLAAAADV
                     ILETFPPGYLTSLGFGYEALREANPGLILCALTPFGQTGPWRDYLSSDLLHMAAGGEM
                     ASCGYDEVDVPNAPPIAPGGGNAWHMGCHYAYMATMAALVYRTVSGEGQYIDVSIHEA
                     CALTTEAAVANYIYRGEVLRRQTGRHHAAGPTPRTQFRAKDGMYVTALISGGLNPRNV
                     RNLANLMDSFGMAGDLKDPKYQDPAVIAANASYIIDELVAGFIA"
BASE COUNT          589 a          915 c          955 g          556 t
ORIGIN      
        1 atgggcggcg gcggcggctc gacggtgacc gccggcggtc tgctgctgcg tggcgagccg
       61 gatggaaact tcgtcgtgct gaatgcgaaa tcgggcgagg tgctatggaa gttccaaacc
      121 gggttcggcg ccgatgcacc gccggcggtg tatgaggttg acggccagca gtatatcgcg
      181 atcgttaccg gtggaaattc cctgcaaggc agcgccaccg gcgacgcatt atgggtgttc
      241 ggcatgaatg gacagctatc cccgctgccg ccaccgcagc cgccatcgag aatcgccggt
      301 ccgctcgggc cgatcgctga tgggaccagc acaatcaaga ttggcgacaa caacgtagag
      361 tatgcctact ggccggctcg cacccgggtc aaggcgggaa ccgccgtgac cttcaccaat
      421 gtcggcgaca tcatgcacac cgcgaccgca gccaccgcgg cggggtcggt tgccgacaaa
      481 tgggataccg gtgcactggc gaaaggggaa tccaagagca tctcgttcac cgaacttggc
      541 acgtattact atatctgcac cccgcatccg tggatgtatg ggcaggtgat tgtcgaagcg
      601 ccgtaggcgg ccgcgacgac gttcgaacca gcggccccgg gcgagatatt cggcgctcag
      661 ggccgctgct cttccttccg cgcgccgatc caagtaaacg actcggttat ctgtgacgca
      721 ggataacgaa atcatgtctc gactgcaggc cctcgagaac attcgcgtcg tggacttcag
      781 ctgggtgcgg gccgggccat gggccacacg ttggctcggg gcgcttgggg cggagatcat
      841 aaaaatcgag tggccggagt ctgagcgcgg caggctgccg agttccacga cgccgcagca
      901 tctcgaggtc aacctgaata cttcgggaaa cttcaacgat accaacgtca acaagaaaag
      961 cgtcagtctt aacgtccgaa gtcccaaggg gctggagatc gtgaagcggc tgatcgccgt
     1021 ctccgatatc gtgatcgaga attttagctc ccgcgtgttg cggaactggg gccttggcta
     1081 tgacgagctt tgcaggatca agcccgatat cgtctatgta tccatgtccg gctacggcca
     1141 caccggccgc aatcaccact acaccacgtt tggtcctgtc gcccaggcgg tctcggggct
     1201 gacctatctt tccggcatgc ccgacaggcc gcccgcaggc tggggctggt cgtatatgga
     1261 cgataccggc ggcatgtacg gggcgatgtg tgccctgacc ggcctctacc atcgcaacat
     1321 gacgggacac ggccagcaca tcgatcaggc tcagatggtg tcgagcgttc ccctcaacgg
     1381 tccggcactg ctggacttca cggtgaacgg gcgcggatcc cgccgggtcg gctatccccc
     1441 cggcaaccga gcgcattggc ctggcacacc tttggtcaac aactaccgtg gtccaacggt
     1501 cgcgccccat aatgctttcc ggacccagcc cggcggctat aacgactggt gcgtgatcgt
     1561 ctgccattcc gatgaggatt ggcagcgact ggtgcaggtg atgggcgcac cggcgtgggc
     1621 cgcctcggca aaattcgtca ccgctgccgg acgcttgcag caccaggcgg aactggacga
     1681 gcagatcgag gcctggagca tgacgctgga taaatacgag ctgacggagc gttgccagga
     1741 agccggtgtt cgcgcccttc cggtgcaaag tgccgaagac cgggtggagc atgaccctca
     1801 gttgcgccac cgccagatgt atctgccgat cgagcatccg gcgctcggca cgcacaaagt
     1861 acagaatgcc ccatttcaac tttcagaaac gccggcttct aaccgcctgc cgagccccct
     1921 gatcggtcag cacacgcggg agatcgtgga aggcttgctg ggctatagcc acgaagaggt
     1981 tcgcgccggg ttcgccgacg gcacattctg gccgacgcag cgggctcgtt ttccatatat
     2041 ggacgagatg ctgcaatgaa tgaagcgttg gggaatgaag cgttggggcc gctggccggg
     2101 cttaggatcc tggaacttgc ggatgaaaag ggccagtttt gcggcaagct tctcggcgat
     2161 ctcggggccg atgtggtcaa gatcgaaccc ccttccggcg agcgctgccg ccacatcggg
     2221 cctttccttg acgacatccc ccacccggag cgcagtctgt cgttctggta ctacaacacg
     2281 tccaaacgcg gcatcaccct gaacctggaa accgccgatg gccaaacgct tttcgggcgc
     2341 ttggcggcgg cggcggacgt gatcctggaa acgttcccac cgggctacct gacctcgctc
     2401 gggtttggtt atgaggcctt gcgtgaggcc aaccccgggc tgatcctgtg cgccctgacg
     2461 ccattcggac agaccggtcc gtggcgggat tacctgtcca gcgacttgct ccatatggca
     2521 gcgggtggcg agatggcgtc ctgcggctat gacgaggttg acgtgccgaa cgcgccgccg
     2581 atcgcgcccg gtggcggcaa cgcctggcac atgggatgtc actacgcata tatggcaacc
     2641 atggccgcat tggtttaccg aaccgtttcg ggggaggggc agtatatcga tgtctcaatt
     2701 cacgaggcct gcgcgctgac gaccgaagct gcggtcgcca actacattta tcggggagag
     2761 gtgctgcggc gccaaaccgg tcgccatcat gctgccggcc ccacgcctcg cacgcagttt
     2821 cgcgccaagg acggcatgta cgtcacggcg ctgatatcgg gtgggcttaa tcccaggaac
     2881 gtcaggaacc tggcaaacct gatggacagc ttcggcatgg cgggcgatct caaggatccg
     2941 aagtaccagg atccagccgt aatcgcggca aatgcgtcgt atatcattga cgaactggtc
     3001 gcgggattca tcgcc
//
LOCUS       DASWQN010000669         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_987279, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000669 DASWQN010000000
VERSION     DASWQN010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987279"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..>1793)
                     /locus_tag="VGC82_11370"
     CDS             complement(256..>1793)
                     /locus_tag="VGC82_11370"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HEY0423912.1"
                     /translation="PSLPDNPDGLPIGFARGAPQIDPRDGTSLINPATGKVMTEFGLT
                     CAACHTGQIVYGKTAMTIDGGSAMLDLGKLRTAVGIAILRTAVLPDRFDRFADRVLGF
                     DSTPTAREQLRKQFRLAVAGVEEQIKLELSVASGSVEEGVGRLDALNRIGNQVFSLDM
                     RDRNGRPNRENYAPTNAPVHFPHIWDAIWFSWAQYDSSIQRPMVRNAGEAMGVGALVN
                     LSNQGQSLYTSSVDFAALHRIEGALSGPYAPGHTPLGDRRFTGLAAPRWPAFLPKIDS
                     QLAAKGEVLYAGMCAGCHLPAPNTAAFWDARFWRPADDNPEAHSFLDLPLIPVLEIGT
                     DPAEAAVLGSRMVTSFPFLDIRTQVFGRALGDVVEKVAQRWYEGQTPPLSAAMQDEMN
                     GYRPNGIRAPMAYRARPLNGIWATAPYLHNASVPNLYALLSPLAERPRVFWLGNRDYD
                     PKTVGYNSTDPVEGGFQLDTSRSGNSNKGHEFDTGPIGNGIIGRRLAPEERLALIEYL
                     KTL"
BASE COUNT          328 a          559 c          588 g          318 t
ORIGIN      
        1 ttcgtcggca tgacgaagtg gcaaggcggg ctgagatagc ggtggaacga gtgaccagag
       61 cggtttcacg gtgagaggaa ccgagttcta ttgtgcctgt aaagaaagtc gtggatggcc
      121 acgccaagca cggccatggc acctggatgc caggtgccac accggctcgc atctcttcca
      181 gtcagcgtga aagcgctttg agagcgtaaa acaaacacca accattttct gcctgtacag
      241 tcgtagttga gcggcttaaa gcgtcttcag atactcgatc agggcgagtc gctcctcggg
      301 cgccagccga cggccgatga tgccgttgcc gatcggccca gtatcgaatt cgtgcccctt
      361 gttgctgttg ccggaacgcg acgtgtcgag ttggaagccg ccttcgaccg gatcggtgct
      421 gttgtagccg acagtcttcg ggtcgtagtc gcggttgccc agccagaaca cacgcggacg
      481 ctccgcaagc ggcgacagca gagcatacag gttcggcacc gaagcgttgt gaagataggg
      541 cgccgtggcc cagatgccgt tcaacggccg ggctcggtat gccatcggcg cgcgaatgcc
      601 gttgggccgg tagccattca tctcatcctg catcgcggcg gatagcggcg gggtctgacc
      661 ctcgtaccag cgctgtgcca ccttctcgac cacgtcgccc agggcacgcc caaacacctg
      721 tgtcctgata tcgagaaacg ggaaactcgt gaccatgcga ctaccgagca cggccgcctc
      781 tgccggatcg gtgccgattt ccaaaacggg gatcagcggc aggtcgagga agctatgggc
      841 ttccgggttg tcatcggccg gacgccagaa cctggcatcc cagaacgcgg cggtgttggg
      901 cgcgggcagg tgacacccgg cgcacatccc ggcatacagc acctcgccct tggctgccag
      961 ttgggaatcg atcttcggca ggaaggccgg ccagcgcggc gccgccagcc cggtgaagcg
     1021 ccggtcgccc agcggcgtat ggcccggcgc ataggggccg gacagggctc cctcgatccg
     1081 gtgcagcgcc gcgaagtcga ctgaggaggt gtacagcgac tggccctggt tcgacaggtt
     1141 caccagcgcg ccgacgccca tcgcttcgcc ggcgttgcgc accatcgggc gctggatgga
     1201 actgtcgtac tgcgcccaac tgaaccagat cgcgtcccag atgtgaggaa aatgcaccgg
     1261 ggcgttggtg ggcgcgtagt tctcgcggtt tggccgccca tttctgtcgc gcatgtccag
     1321 ggagaagacc tggttgccga tgcggttgag cgcatcgaga cgcccaacac cttcctccac
     1381 gctgcccgac gccacgctga gttcgagctt gatctgctcc tccacgccgg caactgccag
     1441 cctgaactgc ttgcgcaact gctcgcgggc ggtaggcgtc gaatcgaaac cgagcacccg
     1501 gtcggcgaat cgatcgaagc gatccgggag gaccgcggtg cgcaggatgg cgatgccgac
     1561 cgccgtccgc agcttgccca ggtccagcat cgccgatcca ccatcgatgg tcatggcggt
     1621 cttgccatag acgatctgcc cggtgtgaca ggcggcgcag gtcaatccga actcggtcat
     1681 gactttgccg gtcgccggat taatcaagct cgtgccgtcg cgcgggtcga tctgtggtgc
     1741 gccacgcgca aatccgatcg gcaggccgtc aggattgtcg ggcaggctgg gtg
//
LOCUS       DASWQN010000670         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1251770, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000670 DASWQN010000000
VERSION     DASWQN010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251770"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..937
                     /locus_tag="VGC82_11375"
     CDS             38..937
                     /locus_tag="VGC82_11375"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEY0423913.1"
                     /translation="MALLPFDDRDGWLWLDGSLVPWRDARLHVLSHGLHYASAVFEGE
                     RAYSGNIFRLRDHTERLINSGRILSFEIPYSAEQIDAASNAVLQANGLTDGYVRPIAW
                     RGSEQLAVSATGTSVHLAIGAWPWPSYFGTERMQGIRMAEAEWRRPPPETAPTKAKAT
                     GLYMIASLSKVKAEQEGYADALMLDWRGLVAEATGANIFFVIDGDLHTPVPDCFLDGI
                     TRRSVMSLARRNQMKVVERQIEPAELTRATEVFLAGTAAEVTAVREIGPHRFTPGRIT
                     ETLMQDYDRLVQQPPESVARMLA"
     gene            complement(1037..1489)
                     /locus_tag="VGC82_11380"
     CDS             complement(1037..1489)
                     /locus_tag="VGC82_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY0423914.1"
                     /translation="MGFSPLTETLLTRADAICQGRGVRLTDLRRQVLGLILDRDAPTG
                     AYDLLDQLRSTRHGAAPPTVYRALEFLREQGLVHKLERLSAFVGCIADEDHDHAAQFL
                     ICRSCGRVTELEDHELAHALDDAARRLGFTVGKATIEAEGQCAACAGT"
     gene            1536..1943
                     /gene="hisI"
                     /locus_tag="VGC82_11385"
     CDS             1536..1943
                     /gene="hisI"
                     /locus_tag="VGC82_11385"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY0423915.1"
                     /translation="MNENAISPVLEAIRFDANGLVPAVAQQHDTGEVLMMAWMNAEAV
                     RETLATGRVCYFSRSRGRLWRKGETSGQTQHLKELRVDCDGDTLLLLVHQDGVACHTG
                     RRNCFYRAFRDGALVEIAAPEVSPDALYGHAHG"
     gene            1936..>2864
                     /locus_tag="VGC82_11390"
     CDS             1936..>2864
                     /locus_tag="VGC82_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0423916.1"
                     /translation="MAETTPVTVLTGFLGAGKTTVLNHLLRQPELARTAVLVNEFGEI
                     GLDHLLVEKLDDSTVLLNAGCLCCTIRGDLARVLREMLPRARRGDIARIVIETTGLAD
                     PVPILATLMTDPVVVSVYRLDGIVTVVDAVNGAAHLDQQEEAVRQVAVADRIIVSKFD
                     LADTGPLHERLRALNPGAPVRQAAQGIVDPGFVLGAGLFDPSAKIPDVAGWLNAAAFE
                     AAPEHHHHDRNCHDARISAFCVTLDQPLDWPGLAMWLEMLVASRGENLLRVKGILNLR
                     GYDRPVAIHAVRHLLHPPVKLAAWPKGDPRTSRL"
BASE COUNT          484 a          918 c          981 g          481 t
ORIGIN      
        1 cggcactgaa cgcaacaaaa ttacggagag gacggccatg gcgttacttc ctttcgacga
       61 ccgggacggc tggctttggc tggacggttc cctggttccc tggcgggacg ccaggctgca
      121 tgttctatcg cacggtttgc actatgccag tgcggtgttt gaaggcgaac gggcctactc
      181 gggcaatatt ttccgcctgc gtgaccatac cgaacgcttg atcaactccg gccgcatcct
      241 gagcttcgaa atcccctact ccgccgaaca gatcgacgcg gccagcaacg ccgtgctgca
      301 ggccaacggc ctcaccgacg gctatgtgcg cccgatcgcc tggcgcggat cggagcaact
      361 ggcggtatcg gcgaccggca cctcggtcca tctggcgatc ggcgcctggc cgtggccaag
      421 ctacttcggc accgagcgga tgcagggcat tcgcatggcc gaagccgaat ggcgccgccc
      481 gccgccggaa accgcaccca ccaaggcaaa ggcgacggga ttgtacatga ttgcctcgct
      541 gtcgaaggtg aaggcagagc aggaaggcta cgccgacgcc ctgatgctgg attggcgcgg
      601 actggtcgcc gaggcgaccg gggccaacat tttcttcgtg atcgacggcg atctgcatac
      661 cccggtaccg gactgcttcc tcgacggcat cacccgccgc agcgtcatga gcctggctcg
      721 gcgcaaccag atgaaggtgg tcgagcggca aatcgagcct gctgaactga cccgcgccac
      781 cgaggtgttc ctggccggaa ccgccgccga ggtgaccgcc gtgcgggaga tcgggccgca
      841 ccgcttcacg cccggaagga tcaccgaaac gctgatgcag gactacgaca ggctcgtgca
      901 gcaaccgccg gaatccgtcg cgcgcatgtt ggcctgactg cctgcgtcgg actgcgcatt
      961 cgcgcccatt aggccgggaa acccggcaga tcggagcctg ccggaaaccg aggcgatggt
     1021 tcaggattgg tgaaaatcac gtcccagcac aggcggcgca ctggccttcc gcctcgatgg
     1081 tggctttccc gacggtgaat cccagccgcc tggccgcgtc gtccagggca tgtgcgagtt
     1141 cgtggtcttc cagttccgtc acccggccgc aggatcggca gatcaggaac tgcgcggcgt
     1201 ggtcgtggtc ctcgtccgcg atgcagccga cgaaggccga cagccgctcc agcttgtgca
     1261 ccagcccctg ttcccgcagg aactccagcg cgcggtacac ggtcggcggt gcagccccat
     1321 gccgcgtgct gcggagctgg tcgagcaggt cataggcgcc ggtcggggcg tcgcggtcca
     1381 ggatcagtcc cagtacttgg cggcgtaagt cggtcagccg gacgccgcgg ccttggcaga
     1441 ttgcgtcggc gcgggtcagc aatgtctcgg tcagggggga aaaccccatg cggcactcct
     1501 tggcggcggc cccgcccgat gcctagatac cattcatgaa cgaaaatgcc atctcgcctg
     1561 tcctggaggc aatccgcttc gacgccaacg gcctggtccc cgccgtcgcg cagcagcacg
     1621 ataccggcga ggtgctgatg atggcgtgga tgaacgccga ggcggtgcgg gagaccctgg
     1681 cgaccggccg ggtctgctac tttagccgca gtcgcggcag gctgtggcga aaaggcgaaa
     1741 cgtccggcca gacgcagcac ctgaaggaac tgcgggtcga ttgcgacggc gacacgctgt
     1801 tgctgctggt gcatcaggat ggcgtcgcct gtcacaccgg gcggcggaat tgcttctacc
     1861 gggcgtttcg tgatggcgcg ctggtcgaaa tagcggcgcc cgaggtttcg cccgatgccc
     1921 tgtatgggca cgcgcatggc tgagaccacg ccggtcacgg tgctgacggg cttcctgggc
     1981 gccggcaaga ccaccgtgct gaaccatctg ctgcgccagc ctgaactggc gcgaaccgcc
     2041 gtgctggtga acgagttcgg cgaaatcggg ctggatcacc tgctggtgga gaaactcgac
     2101 gactccactg tgctgctgaa cgccggctgc ctgtgctgca ccattcgcgg cgacctggcc
     2161 cgcgtcctgc gcgagatgct gccgcgggct cggcgtggcg acatcgcgcg gatcgtcatc
     2221 gagacgaccg gcctggccga cccggtgccg atcctggcga cgctgatgac ggatccagtc
     2281 gtcgtctcgg tttatcgact ggatggcatc gtgacggtgg tcgatgcggt gaacggggcg
     2341 gcgcatctgg accagcagga ggaagcggtt cggcaggtgg cggtggcgga ccggatcatc
     2401 gtcagcaaat tcgatctggc ggacacgggc ccgttgcatg agcgcctccg cgcgctgaac
     2461 ccgggcgcac cggtgcggca ggccgcgcag gggatcgtcg atcccggttt cgtgctgggc
     2521 gcggggctgt tcgatccgtc ggcaaagatc ccggacgtgg ccggctggct gaacgcggcg
     2581 gccttcgagg cggcgcctga gcaccatcat catgaccgga actgtcacga tgctcgtatc
     2641 agcgcgtttt gcgtgacgct ggatcagccg ctggattggc cgggactggc gatgtggctg
     2701 gagatgctag tcgcgtcacg cggggaaaac ctgttgcggg tcaagggcat cctgaacctg
     2761 aggggatacg accggccggt ggcgatccac gcggtgcgtc acctgctgca tccaccggtc
     2821 aagctggccg cctggcccaa aggcgatccg cgcaccagcc gcct
//
LOCUS       DASWQN010000671         3248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1383946, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000671 DASWQN010000000
VERSION     DASWQN010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383946"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VGC82_11395"
     CDS             complement(<1..305)
                     /locus_tag="VGC82_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0423917.1"
                     /translation="MDSIFRIAVVEDDPEISRMVTAYMTDHGFEVTGARNGRELDRVM
                     ASGKIDCVILDVGLPGEDGLSICRRVRGKLSVPIIMVTGRASDTDRIVGLELGADDYL
                     "
     gene            570..1742
                     /locus_tag="VGC82_11400"
     CDS             570..1742
                     /locus_tag="VGC82_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEY0423918.1"
                     /translation="MSLKGAAYIAGAWEHPTRKAPDKSIALLHAECARGALEDAGLTK
                     NDVDGYFCAGDAPGGLGPLSMVDYMNLKCRHVDSTDVVGSSYQVAVGHAMEAIAAGHC
                     NIALITLSGRPRSEGQATGTAPRPGNPAAPEMQFEFPYGPATANLYGMCATRHMHEYG
                     TTSEQLAWIKVAASHHAQHNPHAMLRDVVTVEDVVNSPMVSTPLHRLDCCVISDGGGA
                     LILTKPEIAKTLKRPLVKVIGVGESPKHQMGGEVDLTYTAARWSGPPAWEMAGIKPSD
                     IKYASIYDSFTITVLMQLEDLGFCEKGQGGRFVADGNLISGVGKLPFNTDGGGLCSNH
                     PANRGGITKIIEAVRQLRGEAHPAVQVPNCDLAIAHGTGGALGTRHCGSTVILERE"
     gene            1748..2149
                     /locus_tag="VGC82_11405"
     CDS             1748..2149
                     /locus_tag="VGC82_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY0423919.1"
                     /translation="MAYVARKISFVGTPDTNPETKPYFEGADAGKLMVRRCTSCKKVH
                     WYPRELCPFCFGDCSWEQASGKGKVYTFSVMERANPPYAIGYVTLEEGPSMMTNFVDC
                     DFKALKIGQDVKVTFVKSEGDGPAVPMFTPA"
     gene            complement(2394..>3248)
                     /locus_tag="VGC82_11410"
     CDS             complement(2394..>3248)
                     /locus_tag="VGC82_11410"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639258.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEY0423920.1"
                     /translation="GEVFKGPKQWQAIGVDGDNATYRWSDGSTYVKNPPYFDGITMEP
                     VPVGDIKGARVLAELGDSITTDHISPAGSIRKVSPAGDYLLERQVTQANFNSYGARRG
                     NHEVMMRGTFGNIRIRNEMLKNVEGGMTRHLPSGEEMPIYDAAMRYKKEGVPLVIFGG
                     KEYGTGSSRDWAAKGTMLLGVKAVIVESFERIHRSNLVGMGVLPLVFKPGVDRKTLGI
                     VGDELIDIIGLDTITPRMELSLVIHRANGTVDTVPVICRIDTVDEVAYYQHGGILHYV
                     LRGMAKAA"
BASE COUNT          628 a         1017 c          983 g          620 t
ORIGIN      
        1 aggtagtcgt cggcgccaag ctccaatccc acgatgcggt ccgtatcgct ggcgcgaccg
       61 gtaaccatga tgatcggaac agacaatttg ccccgcaccc ggcggcagat actcaggccg
      121 tcttcgcccg gcaagccaac atccagaata acgcaatcga tttttccact ggccatcacg
      181 cgatcaagct cacggccgtt gcgcgcaccg gtcacctcga acccatggtc agtcatatag
      241 gctgtcacca ttcggctgat ctcgggatca tcctcgacga ctgctatgcg gaaaatagaa
      301 tccatggccc acctttcgcg gcggagactg gcaccgtgtg gtgttcacaa acaagtgcaa
      361 cctgtatttg gacacaaatt gacatattct gtgtcggaac gagctgaccg cgccggtatc
      421 gccggtgcgc cagaatgccg gggccggggt agccgtccgg ctaccgatca gtataatcgc
      481 ccgatgccaa aaccggatcg ccgctggcgg cggtcgtata ggcgtgcagc ggattgccgt
      541 ggaacggccg tccatgaggg gagtgaagca tgagtttaaa aggtgcggcc tatatcgcgg
      601 gggcttggga acatcccacc cgcaaggcgc cagacaagtc catcgccttg ttgcatgcgg
      661 agtgcgccag aggcgcgctg gaagacgcag gcctgaccaa gaacgacgtg gacggctact
      721 tctgcgccgg cgatgcgccg ggtggcctgg gtccgttgtc gatggtggac tatatgaacc
      781 tgaaatgccg tcatgtggac agcacggatg ttgtcggctc gtcatatcag gtggcggtcg
      841 gccatgcgat ggaggcgatc gccgcaggcc attgcaacat tgccctgatc accctgtccg
      901 gccgtccccg cagcgaaggc caggccacgg ggaccgcccc gcgtcccggt aatccggcgg
      961 cccccgaaat gcagttcgag tttccttacg gtcctgccac ggccaacctg tacgggatgt
     1021 gtgcgacccg gcacatgcac gaatacggca cgaccagcga gcaactcgcc tggatcaagg
     1081 tggccgcgtc gcatcatgcg cagcacaacc cgcacgccat gctgcgtgac gtggtcacgg
     1141 tggaggatgt cgtgaattct ccgatggtct ccacgccgct gcatcgcctg gattgctgtg
     1201 tcatcagcga tggcggcggc gcgttgatcc tgaccaagcc ggagatcgcc aaaaccctga
     1261 agcggccgct ggtgaaggtc atcggtgtcg gtgagtctcc caaacaccag atgggcgggg
     1321 aggtcgatct gacctacacc gccgcgcgct ggtccggtcc gccggcatgg gaaatggccg
     1381 ggatcaagcc gtccgacatc aagtatgctt cgatctacga cagcttcacc atcaccgtgc
     1441 tgatgcagct agaggacctg ggattctgcg aaaagggcca gggcggcaga ttcgttgccg
     1501 atggcaactt gatctccggt gtcggcaagt tgccgttcaa taccgacggc ggcggattgt
     1561 gctccaatca tccggccaac cgcggcggca ttaccaagat catcgaggcg gtgcgccagc
     1621 ttcgcggtga ggcccatccc gctgttcaag tgccgaactg cgatcttgct atcgcgcacg
     1681 gcaccggcgg cgcgctcggt acgcgtcact gcggcagcac cgtgattctg gaacgggagt
     1741 gatcgacatg gcctatgtgg caagaaaaat ctcgttcgtc ggcacgccgg acacaaaccc
     1801 cgagaccaag ccgtatttcg aaggtgcgga cgccggtaag ctgatggtcc gccggtgcac
     1861 gagttgcaag aaagtgcact ggtacccgcg cgaactctgc ccgttctgct tcggcgactg
     1921 ctcgtgggag caggcgtccg gcaaggggaa ggtctatacg ttcagcgtca tggaacgcgc
     1981 caacccgccc tatgccatcg gctatgtcac gcttgaggaa ggcccgtcga tgatgaccaa
     2041 cttcgtcgat tgtgacttca aggcattgaa aatcggccag gacgtgaagg taacgttcgt
     2101 gaagtcggaa ggcgatgggc cagcggtgcc gatgttcacg ccggcctgat cgatgcgctt
     2161 ggccctgacg tctggtccaa gcgatttttt ctgcggcagc gaggccaaca ggggcggacg
     2221 atgcgtcgcc gccccggttg ccatcctgtg agggtcaccc agccgtcatc aagttagcac
     2281 gcagaattca acatgcccga ggggtccgac ttcgatagag tcggcccgtt taggagggtc
     2341 agcccgtcca ggagggtcag ccgtccagaa gagtcagccc gtccaggtgg ttgtcaggcc
     2401 gccttcgcca tcccgcgaag gacatagtgc aggataccgc catgctggta gtaggccacc
     2461 tcgtccaccg tatcaatccg gcagatcacc ggcaccgtat cgaccgtccc gttggctcgg
     2521 tggattacca gtgacagttc catccgtggc gtgatcgtgt ccaaaccgat aatgtcgatc
     2581 agttcgtccc cgactatccc cagcgtcttg cgatcgacac ccggcttgaa caccagtggc
     2641 agcacgccca tgccgacaag attggaccgg tggatgcgct cgaagctttc gacgatcacc
     2701 gccttgaccc ccagcagcat cgtgcccttc gccgcccagt cacgcgacga tccagtgcca
     2761 tattccttgc caccgaagat caccagcggc acgccttcct tcttgtagcg catggcagca
     2821 tcgtagatcg gcatttcctc gccggacggc aggtgccggg tcatgccgcc ttcgacattc
     2881 ttcagcatct cattccggat ccggatgttt ccgaacgtgc cgcgcatcat gacctcatga
     2941 ttgccgcgcc gcgcgccgta gctgttgaag ttggcctgcg ttacctgacg ctccagcaag
     3001 tagtccccgg ccggcgacac cttacggatc gaccccgccg ggctgatgtg gtcggtggtg
     3061 atgctgtcgc ccagttctgc cagaaccctg gcgcccttga tgtcgcccac cggcaccggc
     3121 tccatcgtga tgccgtcgaa gtagggcggg ttcttcacgt aggtggaccc gtccgaccag
     3181 cgataagtcg cattgtcgcc atcgacgccg atcgcctgcc actgtttcgg gcccttgaag
     3241 acctcgcc
//
LOCUS       DASWQN010000672         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1911821, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000672 DASWQN010000000
VERSION     DASWQN010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1911821"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..>2580
                     /locus_tag="VGC82_11415"
     CDS             232..>2580
                     /locus_tag="VGC82_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0423921.1"
                     /translation="MTVPDALCPGFGFTAADLASRDGLVRLDKHFLKVLGSADNDLHQ
                     RLLTARAAPDSVAARDESDLVVALGPYLDEFVAELFGIQAETGTLLRETLALDPIHAC
                     KRLFVQRQAVKKYPDPSGFDAVALRAALEARIGAPLTERGFAVHVAASESDPEALDEA
                     LRYAAWATLTASGQAFHHGGTLFRVPHRIDPQHLVPVETIERDGVTMLRLPEHDWRHR
                     EGFALTDHGMNTQQALDQVNYCIWCHTQGKDSCSTGLKDRKTGGFQKSVFGVTLNGCP
                     LDEKISEMHTLRAAGHVLGAFATIAIDNPMMAATGHRICNDCMKACIYQKQEAVDIPQ
                     TETSILKDVLALPWGFEIYALLTRWNPLDIRRPLPRPDSGRKVLVVGLGPAGFTLAHH
                     LMNDGHTIVAIDGLKIEPLSFDVSAPVRDVETLYENLDDRVMAGFGGVAEYGITVRWN
                     KNYLKLVRLLLERRPEFAHFGGIRFGGGATLSMDDAWAMGFDHIALCMGAGKPTVLDI
                     PNGLARGVRQASDFLMALQLTGAAKQSSIANLQLRLPVVVIGGGLTAVDTATESLAYY
                     VRQVEKFALRYRTLVAEKGEMTVRLGWTLEEASIANEFLAHAAAIQAERDTAAAEGRA
                     PALAALLNLWGGATVAYRRRLVDSPSYTLNHEEVAKAMEEAVRFAEGLTPAAVETDAY
                     GHASALRMKRADGSEVALAARAILIAAGTQPNTVLAREDHRIQLDGRYFQAVDENGAK
                     VEPIRGLAKPEQAQVLMHRAENGRFLSFFGDLHPSFFGNVVKA"
BASE COUNT          457 a          851 c          813 g          459 t
ORIGIN      
        1 atccttgacg cgaaacagcg gaggcaggcg cggacattgg ccacaactga taacagccgc
       61 ggttgcgtcg ccaattcgac ctaccggttt ccagtcttcg tccccaattg tatcgacatg
      121 aaggttgcct gaacaggctg cctgtggcat ccgcgccgtt ttcgggccat accggtttgc
      181 cagttcctcc atcttggccc gttcctccac cttgaaaagt gaccttacct cgtgactgtc
      241 cctgacgcgc tttgtcccgg ctttggcttc actgccgccg atctcgccag ccgcgacggc
      301 ctggttcgcc tcgacaagca tttcctgaag gtcctcggca gtgcggacaa cgacctgcac
      361 cagcgcttgc tgaccgcacg agccgcaccc gacagcgtcg ccgcgcgcga tgagtcagac
      421 ctcgttgtcg ccctcggtcc ctatcttgac gagttcgtgg cggagttgtt cggcatccag
      481 gcggaaaccg ggaccttgct acgagagacg ctggcgctcg acccgatcca tgcctgcaaa
      541 cggctgttcg tgcagcgcca ggcggtgaag aaatatcccg atccctccgg cttcgacgcg
      601 gtcgccctgc gcgcggcgct ggaagcccgc atcggcgcgc ccctgaccga acgcgggttc
      661 gccgtgcatg tcgcggcttc ggaaagcgat cccgaggcac tggatgaggc tctccgctat
      721 gcggcgtggg cgacgctgac agcttccggg caggctttcc atcatggcgg gacgctgttc
      781 cgcgtgccgc accgcattga ccctcagcat ttggtgccgg tcgagaccat cgagcgcgat
      841 ggcgtcacca tgctgcgcct gccggaacac gactggcgcc atcgtgaggg tttcgcgctg
      901 accgaccacg gcatgaacac ccagcaggcg ctggatcagg tcaactactg catctggtgc
      961 cacacgcagg ggaaggattc ctgcagcacg ggtctgaagg accgcaagac cgggggattc
     1021 cagaaatcgg tatttggcgt cacgctgaat ggctgcccgc tggacgagaa aatcagcgag
     1081 atgcacacgc tgcgcgccgc cggccatgtg ttgggggcct tcgccacgat cgccatcgac
     1141 aacccgatga tggccgccac cggtcaccgc atctgcaacg actgcatgaa ggcctgcatc
     1201 tatcagaagc aggaagccgt cgatattccg cagacagaga ccagcatttt gaaggacgtg
     1261 ctggcgctgc cctggggctt cgagatctac gcgctgctga cccgctggaa tccgctggac
     1321 atccggcgac cgctgccgcg tccggacagc ggccggaagg tccttgtcgt cgggctggga
     1381 ccggccggct tcaccctggc ccatcacctg atgaacgacg gccataccat cgtcgcgatc
     1441 gatggcttga agatcgagcc gctcagcttc gacgtttccg ccccggtgcg cgatgtggag
     1501 acgctgtatg agaacctcga cgaccgagtc atggcgggat tcggcggcgt ggccgagtac
     1561 ggcatcaccg tccgctggaa caaaaactac ctgaagctcg tgcgcctgct gctggagcga
     1621 aggcccgaat tcgcgcattt cggcggcatc cgctttggcg gcggcgcgac gctgtcgatg
     1681 gacgatgcct gggcgatggg cttcgaccac atcgcgctgt gcatgggcgc cggcaagccg
     1741 accgtgctgg acatcccgaa cggcctcgcc cgcggcgttc ggcaggcatc ggattttttg
     1801 atggcgctgc agctgaccgg tgcggcgaag caatcctcca tcgccaatct gcagctccgg
     1861 ttgcccgtgg tggtgatcgg cggcgggctg accgcggtgg acaccgcgac ggaaagcctc
     1921 gcctactatg tccgccaggt ggagaaattc gccctgcgct accgcacact ggtggccgag
     1981 aagggcgaaa tgactgtccg cttgggctgg accctcgaag aagccagcat tgccaacgaa
     2041 ttcctggccc acgccgccgc cattcaggct gaacgcgaca cagcggcggc cgagggacgg
     2101 gcgccggcgt tggcggcgct tttaaacttg tggggcggcg ccacggttgc ttaccgccgc
     2161 cgcctggtgg acagcccgtc ttacacgctc aaccacgagg aagtggccaa ggcgatggag
     2221 gaagccgtgc gcttcgccga ggggctcacc cccgccgcgg tggaaacgga cgcctacggc
     2281 cacgcctccg cactgcgaat gaagcgtgcc gatggctccg aagtcgctct agccgcacgg
     2341 gcgatcctga tcgccgccgg aacccagccc aatacggtac tggcgcgtga ggaccatcgt
     2401 attcagctcg atggccgcta cttccaggcg gtggacgaga atggtgccaa ggtcgaaccc
     2461 atccgcggtc tggccaagcc ggaacaggcc caggtcctga tgcaccgcgc cgaaaacggc
     2521 cgctttctta gcttcttcgg cgacctgcat cccagcttct tcggcaatgt cgtcaaggcc
//
LOCUS       DASWQN010000673         3849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2571929, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000673 DASWQN010000000
VERSION     DASWQN010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2571929"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..1540)
                     /locus_tag="VGC82_11420"
     CDS             complement(257..1540)
                     /locus_tag="VGC82_11420"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY0423922.1"
                     /translation="MFLTSALRIPDAVAGFLGTLDRPLDPDLLIRSARRQTGMRDFGD
                     IPFIGPLTRLLDSCSRESNLSLVGRSATNWDVVRFLSNLLTVQAAIAQHPEIAKAEIR
                     QPIFITGLPRSGTTFLHRLMLTDEANRAPAVWETIYPSPTAGSRNQRIAKAARQLKAF
                     EWLAPEFRGLHPLEATSPQECSEITAHVFRSLRFDTNYHVPSYRAWLDADVVRHLPAY
                     RFHKQFLQYLRHQDGIERRWVLKCPEHLFALGAIKAVYPDARLVFVHRDPVKVLLSQA
                     KLTEVLRRPFTRSLDPKTLGPHESRRWLDGTRRMMEVGDDGGFPNRICHVHHMDLISD
                     PVSSVEGVYRHFGMQLSQQTTSAIEAYVASRPGGGYGGHSYHFADHGLDEQHERAKFR
                     PYMIRFGVTAEDASRRQPAPSATLVPSAGSQSLQS"
     gene            1750..2382
                     /locus_tag="VGC82_11425"
     CDS             1750..2382
                     /locus_tag="VGC82_11425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0423923.1"
                     /translation="MQEYHLRRRSLIAACSALLSVAMLPARAAGDEAVLAPVQRLIDS
                     LLSIMKAGMATPFQLRFATLGPVIDQSFDLATILKESVGSTWATLPPDQQSMLADAFR
                     RYTVASYVNSFDSYNGQRFDVLPQTRTVGADQVVQTRLIPKAGESHELDYVVRQGSSG
                     WRVVDVLAEGSISRVAVQRSDFRRLLSRGGAQALADSLRAKSADMSGGGS"
     gene            complement(2412..3296)
                     /locus_tag="VGC82_11430"
     CDS             complement(2412..3296)
                     /locus_tag="VGC82_11430"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0423924.1"
                     /translation="MPITITELTHGFAGEVSGIDITQPLTRTQVEILEAGMDRFGVLV
                     YHDQRFTDAQQKAFSLNFGALEQTAGGNLTKAADRRLDADIADVSNLGVDHKPLARDD
                     RRRLFNLGNQLWHSDSSFRAIPAKYSLLSARTVVDAGGNTEFADMRAAYDALDPKTKA
                     EIEDLICEHSLIYSRGTLGFTELSDEEKQMFKPVRQRLVRTHPVTGRKSLYLSSHIGT
                     IVGWPMPEARAFVRDLTEHAVQPRFTYVHKWRQFDLVMWDNRTTMHRVRRFDETQVRD
                     MRRTTVAGDTVTVAQMAA"
BASE COUNT          695 a         1187 c         1222 g          745 t
ORIGIN      
        1 atccacttgg cttgaccatc cacttggcct gaccatccac ggctatccct gcgacgccgc
       61 ttcctgccga tcgagacgct gttcgtgcag gtgcgtgggg ccgcgaccgc gagatggggt
      121 tttgaagctg cgcgcggtgg cgctggatgg accaggatcc acgccacgcc aaccgccaac
      181 gtgtcctgtc ccatgagcgc gatcgagaag cagttggagg ccgcaacggc cggtttcccg
      241 gcaaagccaa cagcccttac gactgcaagc tttggctacc ggcgctcggc accagcgtcg
      301 cgcttggggc tggttggcgg cggctcgcgt cctccgcggt gacgccgaag cggatcatgt
      361 agggacgaaa cttcgcccgc tcgtgttgtt cgtccaggcc gtggtcggcg aaatggtagc
      421 tgtggccgcc gtatccgccg ccgggcctgg acgcgacata ggcttcgatc gccgaggtgg
      481 tttgttgtga cagttgcatg ccgaaatgcc gataaactcc ctccaccgaa ctgaccggat
      541 cgctgatcag atccatgtgg tgcacatggc agatgcggtt ggggaagccg ccatcgtcac
      601 cgacctccat catccgtcgc gtcccatcca accagcggcg gctttcgtgc gggcccagcg
      661 ttttggggtc caggctgcgg gtgaacggac ggcgcaggac ctcggtcagt tttgcctggg
      721 aaagcagcac tttcaccggg tctcggtgaa cgaagaccag cctggcgtcc ggatagaccg
      781 ccttgatcgc ccccagcgca aacagatgtt cagggcattt cagcacccag cgccgttcga
      841 taccatcctg gtgccgcaga tattgcagaa actgcttgtg gaaccgataa gcgggcaaat
      901 gccgcacgac gtcggcatcc agccacgccc ggtacgacgg cacatggtag ttggtgtcaa
      961 acctcaggct gcggaaaaca tgggccgtga tctcgctgca ttcctgcggc gacgtcgctt
     1021 ccagcggatg caggccacgg aattccggtg ccagccactc aaacgccttc agttgcctcg
     1081 ccgctttggc gatgcgctgg tttcggctgc ccgccgtggg ggaagggtag atcgtttccc
     1141 acacagcggg cgcacgattg gcttcgtcgg tcagcatcag ccggtgcagg aacgtcgtcc
     1201 cgctgcgcgg cagaccggtg atgaaaatcg gttggcgaat ctccgccttg gcgatttcgg
     1261 gatgctgagc aattgccgcc tgcacagtca gcaaattgga caggaagcgg acgacgtccc
     1321 agttggtcgc gcttcgtccc accaggctga ggttggattc cctcgaacaa gaatccagca
     1381 gccgggtcag tggaccgata aacgggatat cgccaaaatc gcgcattccg gtttggcgcc
     1441 gggccgatcg gatcaagaga tccggatcga gcggccggtc cagggtgcca agaaagccgg
     1501 ccacggcgtc gggaatccgc aaggcgctgg tcaagaacat caaaaactag tctcccgcta
     1561 acggcgccgt gatcttccac aatcccaatg aatctaaggc cccttcgccg aacttccgga
     1621 cacatcgccg tgagcccgtg cgatcccgaa aggtcgagcc caaggaggtg cgttttttgt
     1681 atctggatga tgtcaggagt agacccacat ggataaatca ttcgtcaaac gcggcagagg
     1741 atcgcttcga tgcaagaata ccatctccgg cgacgtagct tgatcgccgc gtgttcggcg
     1801 ctgctatccg tcgcgatgct cccggcccgc gccgcggggg atgaggcagt tctagcgccc
     1861 gtccagcgtc tgatcgatag cttgttgtcg atcatgaaag ccgggatggc tacgcccttc
     1921 caattacgct ttgcaacgtt ggggccggtt atcgaccaga gtttcgacct ggcgacgatc
     1981 ctgaaggaat cggttggctc aacctgggcg acactgccac cggatcaaca aagcatgttg
     2041 gcggacgcgt tccggcgcta caccgttgcg tcctacgtca acagtttcga cagctacaac
     2101 ggacagcgtt tcgacgtctt gccgcaaacc cggacggtgg gtgcggacca ggtggtgcaa
     2161 acccgcctga ttccaaaagc cggcgagagt catgagcttg actatgtcgt gcgtcaggga
     2221 tcgtccggct ggcgggtggt cgatgtgctg gcggaaggct cgatcagccg cgtcgccgtg
     2281 cagcgttccg acttccgtag gctgctctcg cgcggcggtg cgcaagcgtt ggcggacagc
     2341 ctgcgcgcca aatccgccga tatgtccggt ggcgggagtt gagccgcttc ctgctggcct
     2401 gaaccggacc ctcaggcggc catctgcgcg acggtcaccg tatcgccggc cacggtcgtg
     2461 cggcgcatgt cgcgcacctg cgtttcatcg aaacgccgga cgcgatgcat ggtcgtccgg
     2521 ttgtcccaca tgaccaggtc gaactgacgc catttgtgca cgtaggtgaa ccggggctgc
     2581 accgcatgtt ccgtcaaatc gcgaacgaag gcgcgggctt ccggcatcgg ccagccgaca
     2641 atggtgccga tatgcgacga cagatacagc gacttgcggc cagtgaccgg atgcgtcctg
     2701 accaggcgct ggcgcaccgg cttgaacatc tgcttttcct catccgacaa ctcggtaaag
     2761 ccgagcgtgc cgcgcgagta gatcagcgag tgctcgcaga ttagatcctc gatttcggct
     2821 ttcgtctttg gatccaacgc atcataggcc gcgcgcatgt cggcgaactc ggtgttgcca
     2881 ccggcatcga cgacggtgcg ggcagacagc agcgaatact tcgcgggtat cgccctgaac
     2941 gagctgtccg aatgccatag ctggttgccc aggttgaaca gccgccgccg atcgtcccgt
     3001 gcgagcggct tatggtccac cccgagattc gagacatccg cgatatcggc gtccagccgg
     3061 cggtccgcgg ccttggtgag gttgccgccg gcggtctgct ccagggcacc gaaattcagg
     3121 ctgaacgcct tctgttgtgc gtctgtgaac cgctgatcgt gatagaccag gacaccaaaa
     3181 cgatccatgc cggcctccag gatctcgacc tgggtgcggg tcagtggctg ggtgatgtcg
     3241 ataccggaaa cctcaccggc gaagccgtgg gtcagttcgg tgatcgtgat gggcacggtg
     3301 tttctccttc tattccgctg gcaggcgcct gctggctatt ccgccgccat ggcaggcgcc
     3361 tgctgggctg tcatggcgat cccgttgatc gaggtgcggc gtaagatcgc gcacctcctg
     3421 caaatccctg taccgccgaa cacggtgcat cgtggtgcgg ttgtcccgca tcacaaggtc
     3481 gttgacccgc cgagagcagc ggacagatcg cgccggtgct tccaagtgaa gcatcggagt
     3541 gccacgggcg gttgcccagc gcgtccatgc gccagctatc gtcgctcgcc catggcttgc
     3601 cgctgggtgg gcagctcgcc gctgcgatcg aggttcgcga tgtccccgtt ccgaccgggc
     3661 gaaggcgccg ctcgctgggt ttcgacatct ggccgatccg agtggcttcc gattggctag
     3721 aattgcggct gaagttgcag tgttgctcat cggtcagggc tggtcgcgga gcaccagcaa
     3781 ggcccagcga tccatgcccg cttcgatcgc ggcgacctga tcgacagtca ggggcacgct
     3841 gaggtcggt
//
LOCUS       DASWQN010000674         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_592778, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000674 DASWQN010000000
VERSION     DASWQN010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592778"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VGC82_11435"
     CDS             complement(<1..273)
                     /locus_tag="VGC82_11435"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423925.1"
                     /translation="MQVQTVTGPIDLDQLGRTLMHEHLFIAFPGAEFDPTAKFDRTGF
                     VAEAVQRLKQLRTEHGVRTFVDPCPIELGRDAALMREIAEKSEMNVV"
     gene            complement(286..1836)
                     /locus_tag="VGC82_11440"
     CDS             complement(286..1836)
                     /locus_tag="VGC82_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEY0423926.1"
                     /translation="MNNVVATASGKVRGSVLDGVLVFKTVPYGASTAGANRFRPPQPP
                     DPWSGVRDCLAFTGRAPQAGLRAATRPELENFSGAADPSPETEDCLTVNVWTPGCDLA
                     ARRPVMVWFHGGAFAYGNANSPRTSGSRLAARNDVVVVTVNQRLNIFGHLDLSVFGGE
                     PFRLSGNAGTLDMVASLEWVRDNIAAFGGDPGNVTIFGESGGGAKVSTLLSMPRAKGL
                     FHRAIIQSGAAVRLRTKDRALALTECVLQHRGTAAATVDLLQSVPVADLLAAVGPAQQ
                     AIGPSPTPLFDRYPFGPVVDGDVLPAQPFDPAASDISADVPLIIGDMKTETTSFLATV
                     DKVWDRTLTEAELRQRVEAVAGEHAGRIIELYGQLYPAMNAAERLIAVTTDCNFRIRS
                     LVLAQRRAALRRGRVWMYSFEWETPVFGGKLMSPHAMDVPFVFNTLDLTNATDGRPDA
                     QTLADTMSSVWAAFARNGRPDHPSIPAWPAYDAENRATLVLDKMCRIENDPRGRERRL
                     WQELTGTL"
BASE COUNT          320 a          634 c          609 g          314 t
ORIGIN      
        1 gacgacgttc atctcggact tctcggcgat ttcgcgcatc agcgcggcgt cgcggccgag
       61 ttctatgggg cagggatcga cgaaagttcg gaccccgtgt tcggtgcgca actgtttcag
      121 gcgctggacc gcctccgcca cgaagccggt tcggtcgaat ttcgcggtcg gatcgaattc
      181 ggcgcctgga aaggcgatga acagatgctc gtgcatcaag gtgcgaccga gctgatcgag
      241 atcgatcggg ccggtgaccg tttggacctg catagcgtgc ttcccttaca acgtgccggt
      301 cagttcttgc cacaggcggc gctcgcgtcc gcgtggatcg ttctctatcc ggcacatctt
      361 gtccagcacc agcgtagccc ggttttccgc gtcgtaagcg ggccatgcgg ggatcgaagg
      421 atgatccggc cggccgttgc gggcgaacgc tgcccagacc gacgacatcg tatccgccag
      481 cgtctgggcg tcgggccgcc catccgtcgc gttggtcaga tccaacgtgt tgaacacgaa
      541 gggcacatcc atggcatgcg gagacatcag cttgccgccg aataccggcg tttcccactc
      601 gaacgaatac atccagaccc gacctcgtct caatgctgcc cgccgctgcg ccagcaccag
      661 cgacctgatc cggaagttgc agtcggtcgt caccgcgatc agccgttccg cggcattcat
      721 ggcggggtat agttgcccgt acagttcgat gattcggccg gcgtgctcgc cggcgaccgc
      781 ctcaactcgc tgccgcaact ccgcctcggt cagcgtccga tcccagactt tatcgacggt
      841 cgccaggaaa ctggtcgtct cggtcttcat gtcgccgatg atcagcggaa cgtcggccga
      901 aatatcggac gcggcgggat cgaacggctg cgcgggcagc acgtcgccat ccaccaccgg
      961 cccgaacggg tagcggtcga acaacggcgt cggcgacgga ccgatggcct gctgcgccgg
     1021 tccgacggcg gccagcagat ccgcgaccgg aacggactgc agcagatcta ccgtcgcggc
     1081 ggcggtgccc cggtgctgca gcacgcattc ggtcagcgcc agcgcccgat ctttggtccg
     1141 caaccgcacc gccgcgccgc tttgaatgat cgcccgatga aacaagcctt tcgcccgcgg
     1201 catcgacagc agcgtgctga ccttggcgcc gccgccggac tcgccgaaaa tcgtgacgtt
     1261 gcccgggtcg ccgccgaacg ccgcgatgtt gtcgcggacc cactccagcg atgccaccat
     1321 gtccagcgtg ccggcgttgc cggacaggcg gaacggttcg ccgccgaaca cggacagatc
     1381 gaggtggccg aaaatgttca gccgctggtt gacggtcacc acgacgacat cgttccgcgc
     1441 tgctagtcgg ctgccgctgg tgcgcggcga gttggcattg ccgtaagcga acgcaccgcc
     1501 gtggaaccac accatcaccg gccgcctggc ggccaggtca cagccggggg tccagacatt
     1561 gaccgtcagg cagtcctcgg tttcgggcga ggggtcagcg gcgccggaaa aattctccag
     1621 ttccggccgc gtggctgccc gcaagccggc ctgcggcgcc cggccggtga acgccaggca
     1681 gtcgcgcacg ccggaccatg gatcgggggg ctgcggcggg cggaagcggt tcgcgcccgc
     1741 ggtcgaggcg ccatacggca ccgtcttgaa cacaagcacg ccgtcaagca ctgaaccgcg
     1801 cactttgccg gacgctgtag cgaccacgtt gttcatcgct gtccccccga atcagggcag
     1861 catgccggag gatggcg
//
LOCUS       DASWQN010000675         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1252638, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000675 DASWQN010000000
VERSION     DASWQN010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252638"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VGC82_11445"
     CDS             <1..421
                     /locus_tag="VGC82_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0423927.1"
                     /translation="KGIEDLKAALAASRTLPGANGKVATMGYCLGGRLAFMMAEQSDA
                     DANISYYGVGLDELLSDLTRVTAPLVVHVADKDAFFPPEGRAAVVAATMGHKYAVSYV
                     YPNADHAFARVNGTHWDGRSATIANGRSAEVLVKALG"
     gene            421..1659
                     /locus_tag="VGC82_11450"
     CDS             421..1659
                     /locus_tag="VGC82_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY0423928.1"
                     /translation="MNNPNTAAQAGKGLAKTPGALAGIKVIDLTRVLGGPYCTMILSD
                     HGADVIKIEPPQGDEVRDWGPPFHDGDASYFIGVNRNKRAIALDLSKPEGKAVLLRLL
                     QGADVLIENFKPGSMEKWGIGYEAVLSQRFPKLIHCRVSGFGGDGPLGGLPGYDAILQ
                     AMTGLMSINGDANTGPMRLGTAMVDMGTGLYSAIGILMALHERGQSGQGQFLDMTLYD
                     CGMALLHPQAANFFLNGKRPSGTGNPHPNLVPYDKYPTRTCEIFIASGNNGQFRKLCE
                     LIGRPDMPDDPRFANNGERNINRVALTLALAAAFADQDGKELSLRLIRAGVPAGPVLA
                     VDETTAAPHTAHREMVTELDWYKGIGTPIKFSRTKGGTRRPPPRFNQHGAEVLLEHGY
                     SAAEIDALLNAGVLHAVRRA"
     gene            complement(1836..2585)
                     /locus_tag="VGC82_11455"
     CDS             complement(1836..2585)
                     /locus_tag="VGC82_11455"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEY0423929.1"
                     /translation="MPEPAPVATQAEHVPMVESKQQRPWWSETIGKNRLADYALTALL
                     VLEAMILFIGAPLASMGFRIPLLIGGSVGVPLVLSIVIISSSTGARSLAALASAFAVV
                     GAAYRIDHSSLVTVWLGHLAVIVAVLAISIVVAKAVFAPGRITHHRIEGAVILYLNIA
                     LAFTSVFRLISELDPLAFSQFPAHQSEAAAVGGMLYFSFTTLTSTGFGDILPVNPVAR
                     SMANLESVIGQLYLAILLARLVTMHVEARRY"
BASE COUNT          578 a          794 c          867 g          493 t
ORIGIN      
        1 caaggggatc gaggatctga aagccgcgct ggccgcaagc cggaccctgc ccggcgccaa
       61 cggcaaggtt gcgacaatgg ggtactgcct cggtggccga ctggctttca tgatggcgga
      121 gcagtccgac gccgacgcca acatctccta ctatggcgtt ggcttggacg agttgctgag
      181 cgacctgacc cgagtaaccg cgccgcttgt ggtacacgtc gctgacaagg atgcgttctt
      241 cccaccggaa ggcagggccg cggtggtggc cgcgacaatg ggacacaaat acgccgtcag
      301 ctacgtctat cccaatgccg accatgcatt tgcgcgggtc aacggcactc actgggatgg
      361 ccggtccgct accatcgcca atggacgaag cgcggaagtg ctggtgaagg cgctgggctg
      421 atgaataatc caaacaccgc cgcgcaggct ggcaagggat tggcgaaaac cccgggggcg
      481 ttggcgggaa tcaaggtcat cgaccttacc cgcgtgctcg gcggcccata ttgcaccatg
      541 atcctgtcgg accacggtgc cgacgtgatc aagatcgagc cgccgcaggg cgatgaggtg
      601 cgcgattggg gtcctccatt ccatgacggc gacgccagct atttcatcgg cgtcaaccgc
      661 aacaagcggg cgatcgcgct cgatctgagc aagccggagg gcaaggcggt gctgctccga
      721 ttgctgcaag gcgccgatgt gctgatcgaa aatttcaagc cgggatcgat ggagaagtgg
      781 gggatcggct atgaagcggt gctgtcgcag cggtttccga aattgatcca ttgtcgcgtt
      841 tcggggtttg gcggcgacgg cccgttgggc ggcttgccgg gctacgatgc catcctgcag
      901 gcgatgaccg gactgatgag catcaacggc gatgccaaca ccggcccaat gcggctgggt
      961 acggccatgg tcgatatggg caccggcctg tattcggcga tcggcatcct gatggcgctg
     1021 cacgagcggg ggcaatcggg tcagggacag tttctcgaca tgaccttgta cgattgcggc
     1081 atggcattgc tgcatcctca ggcggcgaac ttcttcctca acggcaaacg cccgtcgggg
     1141 acgggaaatc cgcatcccaa cctggtgccg tacgataagt atcccacccg gacctgcgag
     1201 atattcatcg ccagcggcaa taacgggcaa tttcgcaaat tgtgcgagtt gatcggccgg
     1261 ccggatatgc cggacgatcc gcgcttcgcc aacaatggcg aacgcaacat caatcgtgtg
     1321 gcgctgacgc tggccctggc cgcggctttt gccgaccagg atggcaagga attgtcgtta
     1381 cggctgatca gggccggggt gccggctgga ccggtcctgg cggtggacga aacgacggcg
     1441 gcgccacata ccgctcatcg ggagatggtg actgaactgg actggtacaa gggaatcggg
     1501 acaccaatca agttcagccg caccaaaggc ggcacccgcc gcccgccgcc caggttcaac
     1561 cagcacggcg ccgaggttct gcttgaacac ggttattcag cagcggaaat tgatgcgctg
     1621 ctgaacgccg gagtgctgca cgccgtccgg cgtgcatagt caggctatgt gatccgcacg
     1681 aagcaccata aagccacctg ctaatatgcg gacagattca actccgtatg cgggttgcgc
     1741 ataagctgag cgcgtatctc atcacgttgc aatttcatgt cccttcaatg ggaagagcgg
     1801 tatggagaaa ctgcagtatg gccggttatc cgctgtcagt atcggcgcgc ctctacgtgc
     1861 atggtgacca atcgggcaag taatatcgca aggtataact ggccaatgac ggattccagg
     1921 ttcgccatgc tgcgcgccac gggattgacc ggaaggatgt cgccaaatcc ggtcgaagtc
     1981 agtgtcgtaa agctgaaata cagcatcccg ccaaccgcgg cggcttcact ctggtgcgcc
     2041 gggaattgac tgaaagcgag gggatcgagt tccgagataa ggcgaaagac ggaggtaaat
     2101 gccagggcga tattcaggta aaggatcacc gcgccctcga tgcgatggtg ggtgatgcgg
     2161 cccggcgcga aaaccgcttt cgcgacgacg atcgagattg ccagcacggc gacgatcacc
     2221 gcgaggtgac ccagccaaac cgtaacaagc gacgaatgat cgatacgata cgcggcgccc
     2281 acgacggcaa aggcgcttgc gagcgccgcc agggatctgg cgccggtact cgacgaaatg
     2341 atcacgatcg agagaacaag cggtaccccg accgatccgc ctatcagcag cgggatccga
     2401 aagcccatgc tcgcaagcgg cgcgccgatg aaaaggatca tcgcttccag cacgagcagc
     2461 gcggtgagtg cgtaatccgc aagcctgttt ttaccgattg tttcggacca ccacggccgc
     2521 tgctgtttgc tttccaccat cggcacatgc tccgcttgcg tggcgaccgg tgccggctcg
     2581 ggcattggct ccgagccaac ccttcgttgc ggtgtggcaa actgatccgc aatggtgtct
     2641 gctacccgtc agagcctcca aagcctcatc catcggtctg gtaatacatg cggaacgcgt
     2701 ggatgccgac cttcgtcggc atgacgaggt gg
//
LOCUS       DASWQN010000676         3602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1912529, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000676 DASWQN010000000
VERSION     DASWQN010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912529"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /gene="leuB"
                     /locus_tag="VGC82_11460"
     CDS             complement(<1..1091)
                     /gene="leuB"
                     /locus_tag="VGC82_11460"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY0423930.1"
                     /translation="MAANKKLLILPGDGIGPEVMREVRRVIDWMDRRRMVTFDVSDGH
                     VGGSAIDTYGTPITDETMQKAKDADAILFGSVGGPKWDKLGFDLRPEIAILRLRKELD
                     LFANLRPAIVLDPLVDASTLKADVIRGLNIMIVRESTGGIYFGEPRGVETLPDGQKRG
                     FDTETYTTNEIERVSRVAFELARKRQNRVCSVEKANVMQSGLFWRQTVTALQAREFPD
                     VELTHMYADNCAMQLVRNPRQFDVIVTSNLFGDLLSDLASMLTGSLGMLPSATLGAVQ
                     RSGKRHALYEPIHGSAPDIAGKGIANPLAQTLSFAMLLRFSFGMDEDADLIEKACVNV
                     LKNGLRTADIMAPGSARVGTGVMGESILR"
     gene            complement(1154..1774)
                     /gene="leuD"
                     /locus_tag="VGC82_11465"
     CDS             complement(1154..1774)
                     /gene="leuD"
                     /locus_tag="VGC82_11465"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY0423931.1"
                     /translation="MEPFTTLTAVAAPMPMQNIDTDKIIPARFLKTIKRSGLGVHLFD
                     PLRFDKNGDEKPDFILNQEPFRNAQVIIAHENFGCGSSREHAPWALLDFGIRCIIAPD
                     FADIFYNNSFKNGILPIRLPRAVCDQLMDDTRLGGNARVTIDLARQVVIRPNGEEIGF
                     EIDPFRKHSLLNGLDDIGQTLQHGTEIDGFEAARRESQPWLPSTAA"
     gene            complement(1845..3263)
                     /gene="leuC"
                     /locus_tag="VGC82_11470"
     CDS             complement(1845..3263)
                     /gene="leuC"
                     /locus_tag="VGC82_11470"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY0423932.1"
                     /translation="MSASRPRTLFDKIWDSHVVEKLPDGTCILYIDRHLLHEVTSPQA
                     FEGLRLAGRKLRRPDMTIAVADHNIPTIGSRAPSDIKEEDSRIQVETLEKNVAEFGVP
                     YIPILDVRQGIVHIIGPELGLSLPGTTIVCGDSHTSTHGALGALAFGIGTSEVEHVMA
                     TQTLLQNPAKNMQIDVSGTLGTGCSAKDIVLAIIGKITTAGGNGHVIEYTGDTIRALD
                     MAGRCTVSNMSIEAGARAGLIAPDQTTFDYVKGREFAPKGEAYEQAVAYWRTLPTDPG
                     AHFDTIVRLTTAEIAPMVTWGTTPGAVVPVTGVVPNPSDEPDEGKRAQLLRMLEYMAL
                     TPGQAMQDLPIDVVFIGSCTNGRIEDIRAAAGIAKGRRVADGVQALVVPGSGLVKRQA
                     EEEGLDRILLEAGFEWRDPCCSMCLGMNPDKLTPGQRCASTSNRNFEGRQGQGGRTHL
                     LSPAMAAAAAVTGRLTDVRQMG"
     gene            complement(3423..>3602)
                     /locus_tag="VGC82_11475"
     CDS             complement(3423..>3602)
                     /locus_tag="VGC82_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423933.1"
                     /translation="TTLFGTPVDITVSELAIEAFLPADAETADIMRRVARRGEAPRGP
                     GSAESPDALASQIAN"
BASE COUNT          604 a         1153 c         1159 g          686 t
ORIGIN      
        1 tcgcgcagga tcgactcccc catcacgccg gtaccgacgc gggccgaccc tggggccatg
       61 atgtcggccg tccgcaagcc gtttttcagc acgttgacac aggctttctc gatcaggtcg
      121 gcgtcttcgt ccatgccgaa cgaaaagcgc aacagcatgg cgaaactgag ggtttgcgcc
      181 agcgggttgg caatgccctt gccggcgata tccggtgcgc tgccatggat cggctcatac
      241 agcgcgtgcc gcttgcccga gcgctgtact gcacccagcg tcgccgatgg cagcatgccc
      301 aggctaccgg tcagcatcga cgcaaggtcc gacaacaggt cgccgaacag gttggatgtg
      361 acgatcacgt cgaactgccg cggattgcgg acgagctgca tggcgcagtt gtcggcatac
      421 atgtgcgtca attcgacatc ggggaactcc cgagcttgca gggctgtcac ggtttggcgc
      481 cagaacaggc cggactgcat gacgttggct ttctcgacgc tgcacacgcg gttctgccgc
      541 ttgcgggcaa gttcgaaggc aacccgggaa acgcgctcga tctcgttggt agtgtatgtt
      601 tcggtatcga atccacgctt ttggccatcg ggcagcgttt cgacgccgcg cggttcgccg
      661 aaatagatgc cgcctgtgct ctcgcggacg atcatgatgt tcaggccgcg gatcacatcg
      721 gccttcaggg tggaggcatc gaccagcggg tccagcacga tcgccgggcg cagattggcg
      781 aacaggtcca gttccttgcg caggcggagg atggcgatct ccggccgcag gtcgaatccc
      841 agcttgtccc attttgggcc acccaccgat ccgaacagaa tggcgtccgc gtccttggcc
      901 ttctgcatgg tctcgtcggt gatcggagtg ccgtaggtat caatggccga cccgccgaca
      961 tgaccgtcgg agacatcgaa agtcaccatg cggcggcgat ccatccaatc gatcacgcgg
     1021 cggacctcgc gcatgacctc cggcccgatg ccgtcacccg gaagtatcag aagcttctta
     1081 ttggcggcca tcatggatct ttcgtctgag gcgtcatcgc atggctatac accgaagcgg
     1141 atagggcggt cgatcaagcg gcggtggagg gcaaccatgg ctggctttcg cgtcgggctg
     1201 cctcgaagcc atcgatctcg gtgccgtgtt gcagggtctg gccgatgtcg tccagtccgt
     1261 tcagcagcga gtgtttccga aacggatcga tctcgaagcc gatttcctcg ccgttgggac
     1321 ggatgacgac ctggcgggcg aggtcgatgg tgacgcgggc attgccgccc agcctggtgt
     1381 catccatcag ttggtcgcac acggcgcgtg gcaggcggat cggcaggatg ccgttcttga
     1441 agctgttgtt gtagaaaatg tcggcgaaat ccggcgcgat gatgcagcgg atgccgaaat
     1501 ccagcagtgc ccagggcgcg tgctcgcgcg acgagccgca gccgaagttt tcgtgcgcga
     1561 tgatgacctg cgcgttgcgg aacggctcct ggttcaggat gaaatcaggt ttttcatcgc
     1621 cattcttgtc gaaccgcagc gggtcgaaca ggtgaacccc aaggcccgaa cgcttgatgg
     1681 tcttgaggaa gcgcgccggg atgatcttgt cggtgtcgat gttctgcatc ggcattggcg
     1741 cggcaacagc ggtaagggtc gtgaagggtt ccatgggtat gttccctgcc ctggtgattg
     1801 gttgtgaacg cagcagaccg atggtgccat atcgcggcca tccctcaccc catctgccgc
     1861 acgtcggtca ggcgcccggt gaccgccgcc gccgccgcca tcgcgggcga cagcaggtgc
     1921 gtccggccgc cctggccctg gcggccttcg aagttgcggt tcgaggtgct ggcgcagcgc
     1981 tgaccggggg tcagcttgtc cggattcatg cccaggcaca tcgagcagca cggatcccgc
     2041 cattcgaagc ccgcttccag caggatgcgg tcgagccctt cctcctccgc ctggcgcttg
     2101 accagacccg atcccggcac caccagcgcc tgcacgccat cggccacgcg acggcccttg
     2161 gcgatgccgg cggcggctcg gatatcctcg atccgcccat tggtgcagga gccgatgaag
     2221 accacgtcga tcggcaggtc ctgcatcgcc tggcccggag tcagcgccat gtattccagc
     2281 atccgcagca actgcgcgcg cttgccctcg tcgggctcgt cggacgggtt gggcacgacg
     2341 ccggtcaccg gaaccacagc gcccggtgtc gttccccagg tcaccatcgg cgcgatctcc
     2401 gccgtcgtca ggcgcacgat ggtgtcgaaa tgcgccccag gatcggtcgg cagcgttctc
     2461 cagtaagcga ccgcctgctc atacgcctcg cctttcgggg cgaactcgcg gcccttgacg
     2521 taatcgaacg tcgtctggtc gggcgcgatc agcccagccc gagccccagc ctcgatcgac
     2581 atgttgctga cggtgcatcg ccctgccatg tccagcgccc ggatcgtgtc gccggtgtat
     2641 tcgatgacgt ggccattgcc gccggcggtg gtgatcttgc cgatgatcgc caacacgatg
     2701 tccttggcgg aacacccggt gcccagcgtg ccggacacgt cgatctgcat gttcttggcc
     2761 gggttctgca gcagcgtctg ggtggccatc acatgctcca cctccgacgt gccgatgccg
     2821 aacgccagcg caccgagcgc gccgtgggtc gaggtgtggc tgtcgccaca aacgatcgtc
     2881 gtgccgggca acgacagccc aagctccggc ccgatgatgt gcacgatgcc ctgccgcaca
     2941 tccaggatgg gaatgtaggg aacgccaaat tcggcgacgt tcttctccag cgtctcgacc
     3001 tggatgcggc tgtcttcttc cttgatgtcg gacggcgcgc gggaaccgat ggtcgggatg
     3061 ttgtggtcgg ccaccgcgat ggtcatgtcc gggcggcgca gcttgcggcc ggcgagacgc
     3121 agtccctcga acgcttgcgg gctggtgacc tcgtgcagca gatgccggtc gatgtagagg
     3181 atgcaagtgc cgtccgggag tttctcgacg acatggctgt cccatatctt gtcgaaaagc
     3241 gtgcgcggcc tgctggcgga catatcaact ccgatttcgt acggtgcagg cactgatttg
     3301 aacgaacact tcgtcttgtg caacccgttc tcccgtcagc gggggcgccc cagccatgcg
     3361 agcgccattc atcgccgcaa cgctgggagc ctaagcaagc ctaccgggcg acgtcgcgtc
     3421 gcctaattcg cgatctggct cgccagcgcg tcgggcgact cggcggaacc gggcccccgc
     3481 ggagcctctc ctcgccgggc aacacggcgc atgatgtcgg ccgtctccgc atcggccggc
     3541 aggaacgcct cgatcgccag ttccgatacc gtgatatcga ccggcgtccc aaacagcgtg
     3601 gt
//
LOCUS       DASWQN010000677         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1780681, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000677 DASWQN010000000
VERSION     DASWQN010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780681"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VGC82_11480"
     CDS             complement(<1..558)
                     /locus_tag="VGC82_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0423934.1"
                     /translation="MKNRPTRPIPPHPDVIIEHEQPVWSGRFPLDVVKFRHRRFDGVM
                     SNTRTWELWRRGQAAALLPYDPHTDAVVLIEQFRLPALAAGVDPTLVELPAGLCEENE
                     DPADTIRREMHEEMNLTADRVERIGGYMLTAGGADEFCHLYAGRVVAPAADGEGIVGH
                     GGEAAENEDIRVRVWSAADAIEAAFA"
     gene            complement(555..1910)
                     /locus_tag="VGC82_11485"
     CDS             complement(555..1910)
                     /locus_tag="VGC82_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEY0423935.1"
                     /translation="MTRTGANQQRRRPPEPAEVRIDRMGSEGDGIAHLQDGTPLYLPF
                     TLPNETVVARPLQPRGDGWHAVAEATPNASDARVEPPCRHFGRCGGCVLQHWRDADYR
                     AWKSGLLSHALRQAGFTPPDPLEFVPGLPGERRRIDFAIRRTQGRIVLGLHGQRSAEV
                     IDITDCLVLHPSLMALIQPLRALLRSLQAVRREGSVVINLLDSGPDMLLRSDAALTLP
                     DRTALTAFAALHGLTRVSHANAGDTPETVGLLRPATTVLSSISLRPPPGVFLQATKEG
                     EHAIVEAVLRAMPGKITGKTRVAELFAGCGTLTFALARMVRVAAFEGDTATSGALRQA
                     VNQGGMAGRIEVSQRDLARQPLSAKELGAFAAVVLDPPHGGAAAQIGQLAASGVATVV
                     YVSCNPATLSRDALVLNRAGYKLASATAIDQFLWSARLESVCVFRGDWHAASPALYKR
                     P"
     gene            2044..>2457
                     /locus_tag="VGC82_11490"
     CDS             2044..>2457
                     /locus_tag="VGC82_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0423936.1"
                     /translation="MSDPTAEIARRRTFAIISHPDAGKTTLTEHLLRAGGAIQLAGNV
                     RAKGERRRTRSDWMGIERDRGISVVTSVMTFEYSGRVFNLLDTPGHEDFSEDTYRTLT
                     AVDSAVMVIDAAKGIEARTRKLFEICRMRDIPIITF"
BASE COUNT          426 a          812 c          821 g          398 t
ORIGIN      
        1 cgcgaatgct gcctcgatcg cgtccgcggc ggaccagacg cgcacccgga tatcctcatt
       61 ttccgcagct tcgccgccgt gaccgacgat gccttcgccg tccgccgcgg gtgccacaac
      121 ccggccggca tacagatggc agaactcgtc cgcgccgccc gccgtcagca tgtagccgcc
      181 gatgcgttcg acgcggtcgg ccgtcaggtt catttcctca tgcatttcgc ggcggatggt
      241 gtccgcggga tcctcgttct cctcacaaag gccggcgggg agttcgacca gcgtgggatc
      301 cacgccggcg gccagcgcgg gcaggcgaaa ctgttcgatc aacaccacgg catcagtgtg
      361 cgggtcgtag ggcagcaagg cggccgcctg gccacgccgc caaagttccc aggttcgcgt
      421 attggacatc acgccgtcaa agcggcggtg gcggaatttg acgacgtcga gcggaaagcg
      481 gccggaccac acaggctgtt catgttcgat gatgacatcc ggatgcggcg ggatcggccg
      541 ggtcgggcga tttttcatgg gcgcttgtaa agggcgggcg acgcggcgtg ccaatcgccg
      601 cggaagacgc agacgctttc caggcgggcg gaccacagga actgatcgat agcggtggcc
      661 gaggccagct tgtagccggc gcggttcaac acaagcgcgt cgcgcgacag cgtcgctggg
      721 ttgcagctga catacaccac cgtcgccacg cccgaagcgg cgagttgccc gatttgcgcc
      781 gccgcgccgc catgcggcgg atcgagcacg acagcggcga aggcacccag ttccttcgcc
      841 gacaatggct ggcgggccag atcgcgttgc gacacctcga tccgtccggc catgccgccc
      901 tggttgaccg cttgccgcag cgcgccggaa gtcgccgtgt cgccctcgaa cgcggcgacg
      961 cgcaccatcc gcgccaatgc gaacgtcaac gtcccgcagc cggcgaaaag ttcggcgacg
     1021 cgtgtcttgc cggtgatctt ccctggcatt gcccgaagca ccgcctcgac gatcgcatgt
     1081 tcgccttctt tcgtcgcttg caggaacacc ccgggcggcg gacggagcga aatactggac
     1141 aggacggtgg tggcgggacg cagcagcccg accgtttccg gtgtgtcgcc ggcgttggcg
     1201 tgagagaccc tggtcaggcc atggagggcg gcgaaagcgg tcagcgcggt gcggtccggc
     1261 aacgtcagcg ccgcatccga acgcagcagc atgtcgggac cggaatccag caggttgata
     1321 acgacggacc cttccctccg gaccgcttgc aaactgcgca gcaaggcgcg caacggctga
     1381 atcagggcca ttaaactggg gtgcagcacc aggcagtcgg tgatgtcgat tacctccgcc
     1441 gaacgctgcc catgcagccc cagcacgatg cgcccctgcg tgcggcggat cgcgaagtcg
     1501 atgcggcggc gctcaccggg cagtcctggg acgaactcca gcgggtcggg cggggtgaag
     1561 ccggcctggc gaagtgcgtg ggacagcaag ccggatttcc acgcccgata gtcggcgtca
     1621 cgccagtgtt gcaacacgca gccgccgcaa cggccgaaat ggcggcatgg cggctcgacc
     1681 cgagcgtcgc tggcattagg ggtggcctcg gcgacagcgt gccagccgtc gccacgcggc
     1741 tgcagcggcc tcgcaacgac agtctcgttg ggcaacgtaa atggcaggta aagcggggtg
     1801 ccgtcctgca ggtgggcgat tccgtcgccc tccgacccca tccggtcgat acgcacttcg
     1861 gccggttcgg gcggcctgcg acgttgctgg tttgcacctg ttcgggtcat tcagtcacca
     1921 aggaaggctg tgtttgggca gaaagcccgg cttttcgtgc gcttgcgtca cccgcgcgct
     1981 tcggacgttc cgccggacgg gatcgtgccc tatatatcgc agcaccgcca tgaaagcccc
     2041 ggaatgtccg atcccaccgc cgagattgcc cgccgccgta ccttcgccat catctcccac
     2101 cccgacgccg gaaaaaccac gctgaccgaa catctactgc gggcgggcgg cgcgatccag
     2161 cttgccggca acgtccgagc caaaggcgaa cgccggcgga cgcggtcgga ttggatgggg
     2221 atcgagcggg accgcggcat ctccgtcgtc acgtcggtga tgacgttcga gtattccggt
     2281 cgtgtgttca acttgctgga cacgcccggg cacgaggatt tttccgagga tacctatcgc
     2341 acgctgacgg cggtcgactc cgcggtgatg gtcatcgatg cggcgaaagg gatcgaggcg
     2401 cgcacgcgaa agctgttcga aatctgccgc atgcgcgaca tcccgatcat caccttc
//
LOCUS       DASWQN010000678         5467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_723964, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000678 DASWQN010000000
VERSION     DASWQN010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723964"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGC82_11495"
     CDS             <1..217
                     /locus_tag="VGC82_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423937.1"
                     /translation="GSGDFGAGYGGSGILAPAPAVWLQNPRFEEVSGCGMIRSSLIGA
                     WRPWPGGDTRVLTWSFIYRPRIKPVST"
     gene            662..1681
                     /locus_tag="VGC82_11500"
     CDS             662..1681
                     /locus_tag="VGC82_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423938.1"
                     /translation="MKKILVMVGMMPLLVPAQALAHVKWFCAYDTEVPPLPILDVITR
                     SFSAVAMGFCALMFVAYVVDRATSTTTWAKQLDEAIARHGRYSEIIIRLSVGVLFLVL
                     WHDGDTILMPELKTANSLVPWLQLAISASMIFPGTLILGAAGIMGLYFYAVSEYGAFH
                     MMDYPIIPGLAVYLGLTARPGFNTLRLPVLYCSVAATMMWGAIEKFGYPCWTLPLMAA
                     YPKLMLGFSFDWFMSIAGFVEFSLAFFMITGTALLRCSCLALLMLLVGAIPAFGSNDA
                     TGHLLIIAGLLVMMISGQRTIQMPAVVLRAGILTQAGLLTSGYVLTIVAAFGLYYSSQ
                     YLAGR"
     gene            complement(1810..2958)
                     /gene="lpxB"
                     /locus_tag="VGC82_11505"
     CDS             complement(1810..2958)
                     /gene="lpxB"
                     /locus_tag="VGC82_11505"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY0423939.1"
                     /translation="MPLIYLVTGEQSGDVLGARLMHAIRSRRPDVAFAGIGGDAMAAE
                     GLTTLFPIRALALMGLLEIVPKIFELRTLLRRTIENIEARRPDVLVTIDSPGFTLRVL
                     KALQPTTLPRAHYVAPQVWAWRENRVKHYPGLWDQLLCLLPFEPAFFARHGLPARFVG
                     HPVLESGADTGNAERFRARHGIAAEAKILTVMPGSRQSEVSRLLPILEQTIRLLPVPV
                     VPVVPLAGPVEETVRQQTSSWPVRPILVADTKGKHDAFAASSAALTKSGTSTLELALA
                     NVPMLVTYRINPLSHQIAKRMIKVKYASIVNLLLNRAVVPELLQYESRPDRLAAELGR
                     LLTDSDAADAQRRGFAEALAMLRPASGMPSDCAAEAVLSMLSQSEMRQ"
     gene            3262..3402
                     /locus_tag="VGC82_11510"
     CDS             3262..3402
                     /locus_tag="VGC82_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423940.1"
                     /translation="MSRITNDRLAEKALRTTLLAAEGAAFADPIEGLKALFVPVRLFL
                     NR"
     gene            complement(3645..4337)
                     /gene="ubiG"
                     /locus_tag="VGC82_11515"
     CDS             complement(3645..4337)
                     /gene="ubiG"
                     /locus_tag="VGC82_11515"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEY0423941.1"
                     /translation="MTSETVSVHEVAKFDRLASRWWDPNGPMKPLHRMNPVRIGWIEG
                     LLHGPVRVLDVGCGAGLAAEELARRGHDVLGIDAAGEAIEAARAHAEGLGLALRYRSA
                     IAEDLVAEGARFPLITCLEVIEHVPDQARFVRVLADLLEPGGILVLSTLNRTRRSWVM
                     AKVGAEYVLRMLPVGTHDWRAFITPAELGGLLRAAGLRVGHTTGLLADPLAGRWRLGR
                     DMSVNYMMAATR"
     gene            4417..>5467
                     /locus_tag="VGC82_11520"
     CDS             4417..>5467
                     /locus_tag="VGC82_11520"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY0423942.1"
                     /translation="MARIVMKFGGTSVADLDRIRNVVQRIKREVDFGNQVAVVVSAMS
                     GVTNQLVKWCSELSPMHDAREYDAVVATGEQVTAGLLAIGLQDSGIDARSWQGWQIPL
                     RTDTAHGKARIQSIEGGELIRRMEAGQVAVVAGFQGIAPDNRITTLGRGGSDTSAVAL
                     AAALRADRCDIYTDVDGIYTTDPRIVPKARKLAKIAYEEMLELASVGAKVLQTRSVEL
                     AMNERVRVRVLSSFLDAPPGDDTGTLVVDEDEIVEKENVAGIAYSRDEAKITVRRVPD
                     RPGIAAAIFGALAEQNANVDMIVQNISADGTTDMTFTVGKADLPRARAALAGIKAHIN
                     YAELLEDPDVAKISVV"
BASE COUNT          997 a         1735 c         1688 g         1047 t
ORIGIN      
        1 cggctcgggg gattttggcg ccggctatgg cggctcgggg attttggcgc cggcgccggc
       61 ggtttggctg cagaatcccc gcttcgaaga ggtatcgggg tgcggcatga ttcgcagcag
      121 tctgatcggc gcctggaggc catggcccgg cggtgacacc agagttctca cgtggagttt
      181 tatttaccga ccgcgcataa aaccggtatc aacataataa cgaaactaca gtaacgactt
      241 ctggactgtt ccgcggctga atggcgtcac catagttccc gctgccatcc ggccagtcag
      301 ccggcggtgc aaataggtta cgctgatagt tgttcaggtc cagggccatc acgggcaacg
      361 tggaaatcgt ctccgatacc ttgggccaat tgacaatagg tgaggctatg ttgggatcga
      421 tcattgtttc acttgttgga gttggccttc cggtcgttgc cggaatcggg ctggcgccga
      481 aacgcgaagg cggcgaacgg atgttggcgg atcggtgccg cgatcgcttc cggtcgttga
      541 tgctcgccac ccggccgaga atctgttcgg agcatctgtc cgaggtcctg ccttattcct
      601 ccacgttccg caccggctga agggatcggg tgatcgaacc gcgaccggcg ggatccggac
      661 aatgaagaaa atcctcgtga tggtcggcat gatgccgctg ctggtgccgg cgcaagccct
      721 ggcgcacgtc aaatggtttt gcgcctatga tacggaagtt ccgccgcttc ctattctgga
      781 cgtcattacg aggtcgttca gcgcggtcgc catggggttc tgcgcgctga tgttcgttgc
      841 ctacgtcgtc gaccgcgcga cgagcaccac gacctgggca aaacagttgg acgaggctat
      901 agcccgccac ggacgatact ccgagatcat cattcggctg tctgtcgggg ttctttttct
      961 cgttctgtgg catgacggcg acacgatcct gatgccggag ctgaagactg ccaacagcct
     1021 cgttccgtgg ctgcagttgg cgatttcagc gtcgatgata tttccgggca cgctgatcct
     1081 tggcgccgcc ggtatcatgg ggttgtattt ctatgcggtg tcggaatacg gcgcgttcca
     1141 catgatggat tacccgatca ttcccggcct cgcggtctac ctcgggctga cggcccgccc
     1201 cggtttcaat accctgaggt tgcccgtgct gtattgcagt gtggcggcaa cgatgatgtg
     1261 gggggcgatc gaaaaattcg gctatccatg ctggacattg cctctgatgg ccgcctaccc
     1321 gaagctgatg ctgggcttca gtttcgactg gttcatgtcc attgccggct ttgtcgagtt
     1381 cagtcttgcg ttcttcatga tcaccggcac cgcgctgctg cgttgttcgt gcttggcgtt
     1441 gctgatgctt ctggttggtg cgattccggc gtttggcagc aatgatgcga cgggacatct
     1501 gctgatcatc gccggattgc tcgttatgat gatttcgggc cagcggacca tccagatgcc
     1561 cgcggtagtg ctgcgggcgg gaattctgac acaggccggg cttctgacat ccggctacgt
     1621 gctgacgatc gtcgcggcgt tcggcttata ctatagttcg cagtacctgg cgggacggtg
     1681 atagcgttat cgccctgctt gggcgcggtc atccacgaca ttgcccgcaa gtcggaaaat
     1741 catggtcgcc ggcaaggggc gcggccagga cacaagcggc ggaagcggcg attcgtcatc
     1801 ggataattct cattgtcgca tttccgactg gctcagcatc gacagcacag cctccgccgc
     1861 acaatccgat ggcatccccg atgcaggccg cagcatcgcc agagcttcgg cgaaaccccg
     1921 cctttgtgcg tccgccgcat cgctgtccgt cagtaatcgg ccgagctccg ccgccagccg
     1981 atctgggcgg ctttcgtact gcagcagttc cggaacgaca gcgcggttca gcaacaaatt
     2041 gacgatcgac gcatatttca ccttgatcat ccgcttggcg atctgatgcg acaatgggtt
     2101 gatccgatag gtcacaagca tcggcacgtt cgccagcgcc agttccagcg tcgaggttcc
     2161 ggacttcgtc agcgctgccg aggaggccgc gaacgcatcg tgcttgccct tggtatccgc
     2221 gaccaggatc ggccgcacgg gccaggatga cgtttgctgc cgaaccgtct cctccaccgg
     2281 cccggccagc ggcaccactg gcacgaccgg gacaggcaac agccggatcg tctgctcgag
     2341 gatcggcagc aaccgggaaa cctcgctctg gcggctcccg ggcatgaccg tcaggatctt
     2401 cgcctccgcc gcgatgccat ggcgggcgcg aaatctctcg gcattgcccg tgtcggcgcc
     2461 gctttccagc accggatgtc cgacgaatcg cgccggcagg ccgtgccgag cgaaaaacgc
     2521 cggctcgaac ggcaacaggc acagcagttg gtcccatagc cccggatagt gcttcacccg
     2581 gttctcccgc cacgcccaga cctgaggcgc cacataatgc gcccggggca gcgtggtcgg
     2641 ctgcaacgcc ttcaacaccc gcagcgtaaa ccccggcgag tcgatcgtta ccaatacgtc
     2701 cggtcgccgg gcctcgatat tctctatcgt ccgccgcaaa agcgtgcgca gctcgaaaat
     2761 cttcggcacg atctccagca gccccatcag cgccaaggcg cggatcggaa acagggtggt
     2821 cagtccctcg gcggccatgg cgtcaccgcc gatgccggca aatgcgacat cgggccgccg
     2881 cgaacggatt gcgtgcatca acctggcgcc gaggacgtcg ccgctttgtt caccggtgac
     2941 cagataaatg agcggcatga ccctgagcac cacaagaagg cggcaagttc gtggaatttt
     3001 tccccgggcg caaatccgtg ccgcggccgg cttctcatcg cgctctcgca tcagtgagtt
     3061 gcgactgatt atcaatcgcg atagaagata cctattcttg ggaagccccg cctgcgccag
     3121 gtcgttttac gccccgtctg ttcgacaccc cgccccggtt ccgccgtcgg tctgggcgaa
     3181 gctgaaggat actccttcca tccaatttcc gtccgatcgg acgacgcaag cgcatgcaac
     3241 acgccagccg atgccatcag cttgtcgcgg atcaccaacg accgtcttgc ggagaaggcc
     3301 ctgcggacaa cgctgctcgc ggccgaaggc gccgccttcg cggatccgat cgaggggctg
     3361 aaagcgcttt tcgtgccggt ccgtctgttc ctgaatcgct gaccgccacc ggtcctgaat
     3421 gacagctcga acccaatccc ctgtctccga caggagcaaa ccgggatccg atgcgatgac
     3481 aattgtacgc tcgcttggcg tcaccaccct ggcggccttg atggccgcgc accgaacggc
     3541 cgcggaacca ggcgctgcga ccggttgtcc aatggaccag gcgtggaccg acgcggtcga
     3601 tgtacatctg cggtcgccgc atgaaggcgc ggaccggcgc cctgctaccg cgtggcggcc
     3661 atcatgtaat tgaccgacat atcccgcccc aggcgccatc gcccggccag cggatcggcc
     3721 agcagtccgg tcgtatgccc aacccgcagc ccggccgccc gcaacagccc cccgagttcg
     3781 gctggtgtga tgaatgccct ccagtcgtgc gtgccgaccg gcaacatgcg cagcacatat
     3841 tcagcgccga ccttcgccat cacccaggag cggcgcgtcc ggttcagcgt ggacagcacc
     3901 agaatgccgc caggttccag cagatcggcc agaacccgca cgaagcgcgc ctgatcgggg
     3961 acatgctcga tgacctcaag acaggtgatc agcgggaaac gagcaccctc cgccacaagg
     4021 tcctcggcaa tggccgatcg gtatcgcagg gccaggccca gcccttcggc gtgcgctcgg
     4081 gccgcctcga tcgcttcgcc ggccgcgtcg attcccagga catcgtggcc ccgccgagcc
     4141 agttcctcgg cggccagtcc ggcgccgcag ccgacatcca gcacccgaac gggaccgtgc
     4201 aaaagcccct cgatccagcc aatccgaacg gggttcatcc ggtgtagcgg cttcatcggg
     4261 ccgttcgggt cccaccagcg tgacgccagc cggtcgaact tggcgacttc atgcaccgag
     4321 accgtttcgc tcgtcatggt agctccattg cttgctccga tgggcgcgca tcagtatgtg
     4381 tgcgccggta aatcaaaatg gcaaccgatc tccgctatgg cccgaatcgt gatgaaattc
     4441 ggcggcacgt ccgtcgccga ccttgaccgt atccgcaacg tggtccagcg catcaagcgc
     4501 gaggtcgatt tcggcaacca ggtcgctgtc gtcgtttccg ccatgtccgg cgtcacgaat
     4561 caactggtca agtggtgttc ggagctgtct ccgatgcacg acgcccgcga atacgacgct
     4621 gtcgtcgcta ccggcgagca ggtgacggcg ggcctgctgg cgatcggcct gcaggacagc
     4681 ggaatcgacg cacgatcctg gcagggctgg caaatcccgc tgcgtaccga cacagcacac
     4741 ggcaaggctc ggattcagtc gatcgagggc ggcgaactga tccgccggat ggaggccggc
     4801 caggtcgccg tggtcgccgg cttccaaggc atcgcaccgg acaatcggat caccaccctt
     4861 ggccgcggcg gatcggacac ctcggccgtg gcgctcgccg ccgctttgcg ggcggaccgg
     4921 tgcgatatct ataccgatgt ggatgggatc tacacgacgg acccgcgaat cgtgccgaaa
     4981 gcgcgcaaac ttgccaagat cgcgtatgag gagatgctgg aacttgcgtc cgttggtgcc
     5041 aaagtgctgc agacgcgcag cgtggaactg gcgatgaacg agcgggtgcg ggtgcgggtg
     5101 ctctccagct ttctggacgc gccaccgggt gacgacaccg gcacgctggt ggtggatgag
     5161 gatgagatcg tggaaaaaga gaatgtggcg ggcatcgctt actcccgcga cgaagcaaag
     5221 atcaccgtgc gccgtgttcc ggaccgtccg ggcattgccg ccgccatttt cggcgcgctg
     5281 gcggagcaga atgccaatgt ggacatgatc gtgcagaaca tctccgccga tggtaccacg
     5341 gatatgacct tcacggtggg caaagccgac ctgccgcgcg cccgcgccgc cttggctggc
     5401 atcaaggcac acatcaatta cgccgaactg ctggaagatc cggacgttgc gaagatcagc
     5461 gttgtcg
//
LOCUS       DASWQN010000679         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1912996, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000679 DASWQN010000000
VERSION     DASWQN010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912996"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VGC82_11525"
     CDS             <1..496
                     /locus_tag="VGC82_11525"
                     /inference="COORDINATES: protein motif:HMM:NF023816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0423943.1"
                     /translation="KLVLSLPVLANAMLGGWPDVRSGFLETACAGPRPRREEAELRDR
                     ASAMLRGVGLSRVMDRRADLLEHTAQRFLEIARALTMRPKFVLLDEPAGGLTVAEIEH
                     LGAIIGVIRDSGIGVLLVEHHTDFVFRICDRVTALNLGRTIAHGTTNQVRNDPDVIRV
                     YLGA"
     gene            493..1209
                     /locus_tag="VGC82_11530"
     CDS             493..1209
                     /locus_tag="VGC82_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0423944.1"
                     /translation="MSKLLEVEDLHVAYGKAEVVHGAALFVAEGEFTALLGRNGAGKS
                     TTLHAVSGLIGKRGGQVRFAGKDITAATPRDIVRAGLVQVLEGHRVFHPLTVEDNLLI
                     GTYARNKRGDRSKLDAMYVLFPELAERRQQPASRLSGGQQQILAVAQGIIAEPRLLIL
                     DEPSGGLAPLVIDRILDVARSLSRDGVAILLVEQLVEKALAHADRCYLMATGRIVHEG
                     LAKDLAGSDILHSAYLGGGP"
     gene            complement(1253..>2269)
                     /locus_tag="VGC82_11535"
     CDS             complement(1253..>2269)
                     /locus_tag="VGC82_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HEY0423945.1"
                     /translation="RRDLTATVHAVHRLTPTIIEVVIRAPAAARAFRPGQFYRLQNFE
                     VLAAHDDEHGVGRTVLNMEGLAMTGAWTDPERGLVSVIALEMGGSSDLCSMLRPNEPV
                     ILMGPTGSPTEIHANTTVALVGGGLGNAVLFSIGAATRAAGSKVVYFAGYKALRDRYK
                     IAEIERAADVIVWCCDEAPGFAPNRARPQDRTFVGNIVQAMAAYGSGELGDQAVKLAE
                     ADHMIVIGSHRMMQAVGAARHGTLAPYLKPGHSAIGSINSPMQCMMKEVCAQCLQPQG
                     NPETGERTVVFSCFNQDQALDRVDFPALHERLGLNGTQEKLTAQWIDRTLRRLAVRTE
                     VAAE"
BASE COUNT          378 a          748 c          736 g          407 t
ORIGIN      
        1 caagctggtg ctttccctcc cggtgctggc caatgccatg ctgggcggct ggccggatgt
       61 ccgttcgggg tttttggaaa ccgcctgcgc cggtccccgg ccgcgccggg aagaagccga
      121 actgcgtgat cgcgcctctg ccatgctgcg cggggtcggt cttagccggg tgatggaccg
      181 gcgcgccgat ctgctggaac acaccgccca acggtttctc gaaatcgccc gggccctgac
      241 gatgcgcccc aaatttgtcc tgctggacga acccgcgggg ggcctgacgg tagcagagat
      301 cgagcatctc ggcgccatca tcggcgtcat tcgcgacagc ggcatcggcg ttctgctggt
      361 agaacatcac accgatttcg tatttcgtat ctgcgaccgc gtcaccgcgc tcaacctcgg
      421 ccgaacgatc gcgcacggca cgaccaacca ggtccgcaac gaccctgacg tcatccgggt
      481 atatctgggc gcatgagcaa acttctggag gtcgaggacc tgcatgtcgc ctatggcaag
      541 gcggaggtgg tgcacggggc cgcactgttc gtggccgagg gcgaattcac cgcgcttctc
      601 ggccgcaacg gcgccggcaa aagcacgacg cttcacgcgg tctcgggcct gatcggcaag
      661 cgtggcggcc aggtccggtt cgcgggcaag gacatcaccg ctgccacgcc ccgcgacatc
      721 gttcgtgccg gcctcgtgca ggtgctcgag ggccatcgcg tcttccatcc gctgaccgtg
      781 gaagacaatc tgctgatcgg cacctacgct cgcaacaagc gcggcgaccg gtcgaagctg
      841 gacgcgatgt atgtcctgtt tcccgaactg gccgaacgtc gccagcagcc ggcctcgcgc
      901 ctgtccggtg gacagcagca gattctggcc gtggcgcaag gcattatcgc cgaaccaagg
      961 ttgctgattc tggacgagcc gtcaggcggg ctggcgccgc tggtgatcga ccggattctc
     1021 gacgtcgcgc gttcattgag ccgcgacggg gtggcgatcc tgctggtgga gcaactggtc
     1081 gagaaagccc tggcgcacgc agaccggtgc tacctgatgg cgacagggcg catcgtgcac
     1141 gagggcctgg cgaaggattt ggccggcagc gacattctgc acagtgccta tctgggcggc
     1201 gggccctaac gcccgcgtcg atcggcggcg cgcacgcggg aaatcgcgct gattattcgg
     1261 cggccacctc ggtccgcacc gccagccgcc gcagcgtacg gtcgatccat tgtgccgtca
     1321 gcttctcctg cgtgccgttc agccccaacc gttcatgcag cgccgggaag tccacccggt
     1381 ccagcgcctg atcctgattg aagcaggaga ataccacagt ccgttctccg gtttcgggat
     1441 tgccctgcgg ttgcaggcac tgcgcgcaaa cctccttcat catgcattgc atgggggagt
     1501 tgatcgaccc gatcgcgctg tgacccggct tcaggtaagg cgccagggtt ccatgccgag
     1561 ccgcgccgac cgcctgcatc atgcggtgcg aaccgatgac gatcatgtgg tccgcctcgg
     1621 ccagcttcac cgcctggtct cccagttcgc ctgacccgta ggccgccatc gcctgcacga
     1681 tattgccgac gaaggtccgg tcctgcgggc gggcgcggtt tggcgcgaac ccaggcgcct
     1741 catcgcagca ccagacgata acatcggcgg cacgttcgat ttccgcgatc ttgtaacggt
     1801 cgcgcagcgc cttgtagccg gcgaaataga ccaccttcga cccggccgcc cgggtagccg
     1861 cgccgatgga aaacagcacg gcgttgccaa gtccgccgcc gaccagggcg accgtggtgt
     1921 tggcgtggat ctcggtaggc gagccggttg gtcccatcag gatcaccggc tcgtttggtc
     1981 gcagcatgga gcagaggtcg gaactgccgc ccatttccag cgcgatgacg ctgaccaatc
     2041 cgcgttcggg atcggtccat gcgccggtca tcgccaatcc ctccatgttc agcaccgtgc
     2101 ggccgacacc gtgttcgtcg tcatgtgccg ccagaacctc gaaattctgc aaccgataga
     2161 actgtcccgg acggaatgct cgtgcagcgg cgggtgcgcg gatgaccacc tcgatgatcg
     2221 ttggggtgag ccggtgcacg gcatggacgg tggcagtcag atcgcgtcg
//
LOCUS       DASWQN010000680         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_461468, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000680 DASWQN010000000
VERSION     DASWQN010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461468"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VGC82_11540"
     CDS             <1..937
                     /locus_tag="VGC82_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0423946.1"
                     /translation="EKRRAEKLRLLSAFAAKQEAERAHLQSFVDRFRAKASKAAQAQS
                     RMKRLAKMEPVSATIEERVAPFTIPSPTRPLAPPLMGLDHASVGYGGEPVLRNLNLRL
                     DVDDRIGLLGVNGAGKSTFAKMAAGALDLQAGHMQRERRIKVGWFHQHQIEAMDPADT
                     PLDIIRRERPDDSETAHRSRLAGFGIGFDKQSTTVENLSGGERARLLLNLVAMAAPHL
                     LILDEPTNHLDIDSRRALLDALNDYRGAVILITHDRSLMELVADRLWLAADGTVKPFN
                     GDLDDYARFVLDRAKGGSAPGQMRGNKAKRVKKAA"
     gene            971..1537
                     /locus_tag="VGC82_11545"
     CDS             971..1537
                     /locus_tag="VGC82_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423947.1"
                     /translation="MRIGTWLCVAVLGAASLLVSPSLAANYPTPLEADWIATDFKFHT
                     GEVIPALRLHYTTIGDPGGEPVLILHGTGQSGRAMLTPGFAGELFGSGQPLDAARYFR
                     EASRDYDPAPGLERIAAPLLAINAADDERNPPETGLMAQALARVRNGHLLLIPASADT
                     RGHGTTGLAKFWQQQVREFLAEVPKQPM"
     gene            1724..>2460
                     /locus_tag="VGC82_11550"
     CDS             1724..>2460
                     /locus_tag="VGC82_11550"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0423948.1"
                     /translation="MLSREDNDIVTRVGPDTPMGRTLRMYWVPALLSSEIAEPDCPPV
                     RVRLLGEDLVAFRDTKGRVGLLDEYCPHRRVSLFFGRNEECGLRCIYHGWKFDIDGQC
                     LEMMNEPEENDFSHKVRAAAYPSCEMGGIVWAYLGPPGQQPAPPKFAWTQAPETHRHV
                     TKVIQECNWLQALEGGLDTSHAPILHRLISETSTRGGFKPSNPFVRGKPPKLVVDLTD
                     YGYQYAGVRSLGDDEIHVRTYHFILPF"
BASE COUNT          445 a          792 c          793 g          430 t
ORIGIN      
        1 cgaaaaacgc cgggcggaaa aacttcggtt gctgtcggcg ttcgcggcaa agcaggaggc
       61 ggagcgggcg catctgcaaa gtttcgtcga tcgttttcgc gccaaggcca gcaaagccgc
      121 gcaggcgcaa tcgcgcatga agcggctggc gaagatggag ccagtttccg ccaccatcga
      181 ggaacgtgtg gcgccgttca ccatcccgtc gccgacccgc ccgctggcac cgccgctgat
      241 gggactggac cacgcgtctg tcggatacgg cggcgagccg gtgctgcgga acctgaacct
      301 gcggctggac gtggacgacc gtattggcct gctgggcgtc aacggcgcgg gcaaatcgac
      361 ctttgccaag atggccgccg gggccctgga tctgcaggcc ggccacatgc agcgcgagcg
      421 ccgcatcaag gtcggatggt tccaccaaca tcagatcgag gcgatggacc ccgccgacac
      481 cccgttggac atcatccgcc gtgaacgccc ggacgacagc gagaccgccc accggtccag
      541 gctggctgga ttcgggatcg gcttcgacaa gcagtcaacc acggtggaga acctctccgg
      601 cggcgaacgt gcccggttgt tgctgaacct ggttgcgatg gcagcgccgc atctgcttat
      661 tctggacgag cctaccaacc acttggatat cgacagtcgc cgtgccttgc tggacgcgct
      721 gaacgactat cggggcgcgg tcatcctgat cacccacgac cggtccttga tggaactggt
      781 ggccgaccgg ctttggctcg ctgccgatgg gaccgtcaag ccgttcaatg gagacctgga
      841 cgactacgcc cggttcgtgt tggatcgcgc caaaggcggc agtgcacccg gccagatgcg
      901 cgggaacaag gcgaagcgcg tcaaaaaggc ggcgtgactt tgtcgccgat acggggacgg
      961 ggagaccggc atgaggatcg ggacatggtt atgcgtcgcg gtgctcggtg ctgcctcctt
     1021 gctcgtatcg ccctcgctcg ctgccaacta cccgaccccg cttgaggccg actggatcgc
     1081 caccgacttc aaattccaca ccggcgaagt catcccggcg ctgcgcctgc actacaccac
     1141 gatcggcgac cccggcggcg agcccgtcct gatcctgcac ggcaccggtc agtccggccg
     1201 cgcgatgctg acacctggct tcgctggcga actctttggt tccggccagc cgctggatgc
     1261 cgcccggtac ttcagggagg cgtctcgcga ctacgatccc gctcccggtt tggagcgtat
     1321 tgccgcgccg ctgctggcaa tcaacgccgc cgacgatgaa cgcaacccgc ccgaaaccgg
     1381 attgatggcc caggccctgg cgcgcgttcg aaacgggcat ctgctgctga tcccggcaag
     1441 tgccgatacg cgcgggcatg gcacgaccgg gctggcgaag ttctggcagc agcaagtgcg
     1501 ggaatttctt gccgaggtgc caaagcagcc gatgtaaggg accgcatcct cgttcatcgc
     1561 caggaattcc attaccgcac gagggtcccc tgtaagtgca ggcggcttgt cagaatcggg
     1621 gtctcttgcc cttggttgcg gtccggtgat acgggttgat cgactttaag cgtattgcga
     1681 cgtcttgcct ttcgattggt caacaccagg aggggatacg gccatgcttt cgcgcgaaga
     1741 caacgacatc gtcacccgcg tcggtccgga cacgcccatg gggcgaacgc tgcggatgta
     1801 ttgggttccc gccttgctct cgtcggaaat cgccgaaccc gattgcccgc cggtgcgcgt
     1861 gcgcctgctg ggcgaggatc tcgtggcgtt ccgcgacacc aagggccgtg ttgggctact
     1921 ggatgaatac tgcccgcacc ggcgtgtctc gctgtttttt ggccgcaatg aagaatgcgg
     1981 cctgcgctgc atctatcacg gctggaagtt cgacattgat ggccaatgcc tcgagatgat
     2041 gaacgagccg gaggagaacg acttcagcca caaggtgcgg gctgccgcat acccaagctg
     2101 cgaaatgggc ggcatagtct gggcctatct cggtcccccc ggccagcagc cggcaccgcc
     2161 gaaattcgcc tggacgcagg cgccggagac gcaccggcac gtcaccaagg tgatccagga
     2221 atgcaactgg ctgcaggcgc tggagggcgg gctcgacacc tcgcacgcgc caatcctgca
     2281 ccggctgatc agcgagacgt cgacgcgggg cgggttcaag ccctccaacc ccttcgtgcg
     2341 tggcaagccg ccgaagctgg tggtggacct gacggattac ggctaccagt acgctggcgt
     2401 ccggtcgctg ggggacgacg aaatccacgt ccgcacctat catttcatcc tgccgttcca
//
LOCUS       DASWQN010000681         4044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2440524, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000681 DASWQN010000000
VERSION     DASWQN010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2440524"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="VGC82_11555"
     CDS             complement(<1..160)
                     /locus_tag="VGC82_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate-protein ligase B"
                     /protein_id="HEY0423949.1"
                     /translation="MISCGTPEWRIADGLTEYPAALAEMQARVAAIHAGVAPECVWLV
                     EHPPLYTAG"
     gene            209..294
                     /locus_tag="VGC82_11560"
     tRNA            209..294
                     /locus_tag="VGC82_11560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:242..244,aa:Leu,seq:gag)
     gene            complement(296..478)
                     /locus_tag="VGC82_11565"
     CDS             complement(296..478)
                     /locus_tag="VGC82_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423950.1"
                     /translation="MTRHKVDPLAHAGREPMRRIIAKRWSTTPIRHGIPWTNVTNVRT
                     FALLRSIQATGEAKVS"
     gene            complement(867..1178)
                     /locus_tag="VGC82_11570"
     CDS             complement(867..1178)
                     /locus_tag="VGC82_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423951.1"
                     /translation="MRAVRVLTGGYGISADQPWLPGLPHVAAALATYFVFGGQACVTG
                     LLLATLASFVVYLPLTALTLNGGRAIHADASAPSLTLKAQAALLGLWTLTAWVGAALA
                     A"
     gene            complement(1182..2882)
                     /locus_tag="VGC82_11575"
     CDS             complement(1182..2882)
                     /locus_tag="VGC82_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HEY0423952.1"
                     /translation="MDIERPLSANERIKQASNLLRGTIADGLSHVETGALADDDTQVT
                     KFHGFYQQDDRDLRAERGKQRMEKAFSFMVRMRVPAGILTPKQWLGVEKLAMERANHT
                     IRLTTRETVQFHGILKGNLRPLMQGLHDDMLDTIAACGDVNRNVIAAADPWRRDLHEV
                     VCGLAREISTHLLPRTRAWHEIWLDEEKVSGGIEEDEPILGRTYLPRKFKIAIALPPH
                     NDVDVFAHDLGLIAIVEGNRIVGYNVVVGGGMGMTHGEPETYPRTGDVIGFCKPEHAV
                     DVAEKVVTVQRDHGDRSNRKHARLKYTIDDMGLDRFAGLVNERLAVKLEPPRDYNFTS
                     TGDRLGWAEEADGSAHFTLFVENGRIRGRMMEGLHAVAELGVGRFIMTPNQNLILADI
                     PAASRQAVETILAGHGLDQPVSGLRRSAMACVALPTCGLALAESERYLPDLITRLEDE
                     MERFSLRDDEITIRMTGCPNGCARPYVSEIGLVGRTPGIYNLYLGGAHEGTRLNKLHR
                     RDVDGDGIVAALSPLFASYAKERTGNERFGDYVIRAGIVKETTAGLNFHENLSTEVGN
                     "
     gene            complement(2895..>4044)
                     /locus_tag="VGC82_11580"
     CDS             complement(2895..>4044)
                     /locus_tag="VGC82_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HEY0423953.1"
                     /translation="TLERLNAEVGIKDAAGASVIHVDFARPGADAPSRTRPFEAEITE
                     HGRLSGSRSTSDTHHVAVSLEGSGIDYEPGDSLGVVPSNDPALADAVLKAAGLSGNDT
                     LRSTLIDRLDITTLTGKQIEDFARETGTQAPPAGWAEGRQIIDLLETAPGALTAEQLT
                     ALLRPLPPRYYSIASSRKAVGEEAHLLVAGLRYVSHGRDRGGVASLDMTERHREGDKL
                     KVFLRSNPHFRLPADNDRPIIMVGPGTGLAPFRGFLQEREAIGAGGRNWLVFGHRNYT
                     HDFLYQLELQDWLKSNLLTRLDVAFSRDQPEKRYVQNTLWDSRSDLYAWLKDGAALYV
                     CGDASAMAKDVHAMLLRILADQGKQTEAAAKAELDTIRRDGRYLRDVY"
BASE COUNT          694 a         1349 c         1255 g          746 t
ORIGIN      
        1 tgccggcagt ataaagcggc gggtgctcaa ccagccagac gcattccggg gcgacgccgg
       61 cgtggatggc ggcaacacga gcctgcattt cggcgagggc ggcgggatat tcggtcaggc
      121 cgtcggcgat ccgccactcc ggcgttccgc aggagatcat tgaaccggcg ttattcatcc
      181 gctatacccc gcccctgcct accacggtgc ggtcgtggcg gaatggtaga cgcgcaagct
      241 tgaggtgctt gtgggggtaa cctcgtggaa gttcgagtct tctcgaccgc accaatcaac
      301 tgaccttcgc ttctcccgtc gcctgaatgc tgcggagcag tgcgaatgtg cgcacattcg
      361 ttacgttggt ccaggggatg ccgtgccgga tcggtgtcgt gctccagcgt ttcgcgatga
      421 tgcggcgcat cggttcacgc ccagcatgcg ccagcggatc gactttatgc cgggtcacta
      481 cggcggatgc cggcgctgat cgaccggccg ctgccggtgc cggcggagtg cctggcgacg
      541 ctggacgaat tgtcggcgcc gggttgatgc cgcattacgg ggatccgttc agtcctcgcg
      601 gggttgccgg gccagtaggt ctcatcacgg agaaccggat ggcctcccaa accaatcgct
      661 catgccgttg gctcccaaca cctcgatcct gctccgtgac acttgtcctg cgttcgtgtt
      721 gagaatcgta aagagagaac gcgcccgccg caccaggccc ggctacgctt caaacacctc
      781 ccagcgcgat gcccgccgcc agcagcgaaa cggctccccc cctaaatgtc agccgggctt
      841 gcccatccgg ttccggcttt gcgcgctcaa gccgccaagg cggctccgac ccaagccgtc
      901 aacgtccaca gtccgagcag ggctgcctga gccttcaggg tcaaagaagg cgccgacgca
      961 tctgcgtgaa tggcccggcc cccgttcaac gtcagcgccg tcaacggcag atagaccacg
     1021 aagcttgcca gcgtcgccag cagcaggccg gtcacgcagg cctgcccgcc gaagacgaaa
     1081 tacgtcgcca gagcagcggc cacatgcggc aaacccggca gccacggctg gtcggcggaa
     1141 atgccataac cacccgtcag gacacgaaca gcgcgcatcg atcagttccc cacttcagtg
     1201 gacaggtttt cgtggaaatt cagcccggcg gtcgtttctt ttacgatacc cgctcggatc
     1261 acgtagtcgc caaagcgctc gttgcccgtc cgctccttcg cataggacgc gaacagcggc
     1321 gacagtgcgg caacgatgcc gtcgccatcg acgtcgcggc ggtgcagctt gttcagcctg
     1381 gtgccttcat gcgctccgcc gagatacagg ttgtagatac caggcgtccg gccaaccaat
     1441 ccgatttccg acacgtaggg ccgagcgcaa ccgttcggac agccggtcat gcggatggtg
     1501 atttcgtcgt ctcgcaggct aaatcgctcc atctcgtctt cgagtcgagt gatcaggtcg
     1561 ggcaaatagc gctcactctc ggccagggcc agaccgcagg tcggcaaggc aacgcatgcc
     1621 atcgcactgc gccgcaaccc gctcaccggt tgatccaacc catgtccggc caggatcgtc
     1681 tcgaccgcct gacggcttgc cgccggtata tccgccagga tcaaattctg gttcggcgtc
     1741 atgatgaacc gtccgacgcc gagttccgcg accgcgtgca gcccctccat catccgcccg
     1801 cggatgcggc cgttctccac gaacagggtg aaatgtgccg aaccgtcggc ttcctccgcc
     1861 cagcccaagc gatcaccggt cgaggtgaaa ttataatccc gaggcggttc cagcttcacc
     1921 gcaagccgct cgttgaccag cccggcgaac cgatcgagcc ccatgtcgtc gatggtgtat
     1981 ttcagccggg cgtgcttgcg attggaacga tcgccatgat cgcgttgaac cgtaacgact
     2041 ttctccgcga catccacggc atgttccggc ttgcagaagc cgatcacgtc gccggtccgc
     2101 ggataggttt ccggctcgcc atgggtcatt cccatgccac cgccgaccac gacgttgtag
     2161 ccgacgatgc ggttcccctc gacgatggcg ataagcccga ggtcgtgggc gaacacgtcc
     2221 acatcgttgt gcggcggcaa tgcgatggcg atcttgaact tgcggggcag gtaggtgcgg
     2281 ccaagaatcg gctcgtcttc ctcgatcccg ccggatactt tttcctcgtc cagccagatc
     2341 tcgtgccagg ctcgggtgcg cggcagcagg tgggtcgaga tctcgcgggc caagccgcag
     2401 acgacctcgt gcaggtcccg ccgccatgga tcggccgccg cgatgacgtt gcggttgacg
     2461 tcaccgcacg ctgcgatcgt gtccagcatg tcgtcgtgca gcccctgcat cagcgggcgc
     2521 aggttgccct tcaggatgcc gtggaactgc accgtctcgc gcgtcgtcaa gcgaatggtg
     2581 tggttggcgc gttccatcgc cagtttctcc accccaagcc actgtttcgg agtcagaatg
     2641 ccggccggca cccgcatccg gaccatgaag ctgaaagctt tctccatccg ctgcttgccg
     2701 cgttcggcac gcagatcgcg gtcatcctgc tgatagaacc catggaattt cgtcacctgc
     2761 gtgtcgtcgt cggcaagcgc gccggtttcc acatggctca gaccgtcggc gatggtgccg
     2821 cggagaagat tgctggcttg cttgatgcgc tcgtttgccg aaagcggccg ttcgatatcc
     2881 atgttcgccg tcccttagta cacgtcgcgc aggtagcggc cgtcgcgacg gatcgtgtcg
     2941 agttcggctt tcgccgccgc ctcggtctgc ttgccctggt cggccagaat acgcagcagc
     3001 atcgcgtgca cgtccttggc catcgcgctg gcgtcaccac aaacgtaaag cgcggcgcca
     3061 tccttcagcc aggcatagag atccgaccgc gaatcccaca gcgtgttctg cacatagcgt
     3121 ttctcgggct gatcgcggga gaacgcgacg tccagccgcg tcagcaggtt gctcttgagc
     3181 caatcctgca gttcgagctg atacaggaaa tcatgggtgt agttgcggtg cccgaacacc
     3241 agccagttgc ggccgccggc gccaatcgcc tcgcgctcct gcaggaagcc acggaacggc
     3301 gccaggccag tgccggggcc gaccatgatg atcggccggt cgttatccgc cggcaggcgg
     3361 aaatgcgggt tcgaccggag gaacaccttc agcttgtcgc cctcgcgatg ccgttcggtc
     3421 atgtccagcg acgcaacgcc gccccggtcg cgtccgtgcg acacgtagcg cagtccggcg
     3481 accagcagat gcgcttcctc gcctaccgcc ttgcggctgg aggcgatcga gtaatagcgc
     3541 ggcggcaacg ggcgcagcag cgcggtcagt tgttcggcgg tcaacgcacc gggcgcggtt
     3601 tccagcaggt cgatgatctg gcggccctca gcccaacccg ccggcggcgc ctgggtgccg
     3661 gtttcgcggg cgaaatcctc gatctgcttg ccggtcagcg tcgtgatgtc cagccggtcg
     3721 atcagcgtgc tgcgtagcgt gtcgttgccg gacaggccgg ccgctttcag caccgcatcg
     3781 gccagcgccg gatcgttgga cggaaccaca cccagcgagt ctcccggctc gtagtcgatg
     3841 cccgaccctt ccagcgacac cgcgacatga tgggtgtcgg aggtggatcg gctgccgctc
     3901 aggcgcccat gttcggtaat ctcggcttcg aacgggcggg tccgggatgg cgcatccgca
     3961 ccgggccggg cgaaatcgac atgaatgacg gaggcgcccg cggcatcctt gatcccgacc
     4021 tcggcgttca gccgctccag cgtg
//
LOCUS       DASWQN010000682         2183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_330140, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000682 DASWQN010000000
VERSION     DASWQN010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330140"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VGC82_11585"
     CDS             <1..759
                     /locus_tag="VGC82_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0423954.1"
                     /translation="VGGALAMLKDGLFERFPCNAVYGMHNRPGLPVGRFITGPGARAA
                     GGAFFDITVTGKGAHGAHPQQGIDPVLVACHLGTALQSIVSRNVSAMDTAVLSITRIQ
                     SGDAYNVIPQSAVMAGTVRTLKPEVMTLIEQNMKRLVSSVAAGFGAEALVDFRVIFAP
                     MVNDAAEAIAYGDAAAGLTGEENVRRDGPPGMGSEDFSFMMEQVPGAHINLGNGESAA
                     LHNHKYDFNDEAIPYGVALYAAITEKKLPKFSPD"
     gene            843..1048
                     /locus_tag="VGC82_11590"
                     /pseudo
     CDS             843..1048
                     /locus_tag="VGC82_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
     gene            complement(1094..1759)
                     /locus_tag="VGC82_11595"
     CDS             complement(1094..1759)
                     /locus_tag="VGC82_11595"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY0423955.1"
                     /translation="MKIDTIDPTRTAMIVADMQNDFVAAGAPMETPAARAVVPKLAEA
                     LTICRDAGIRVIYTAHVHRRDGSDMGLFDDMHPPIASRAALVDGTAGVDIYPELAPAP
                     GEHVIKKHRYSGFFGTDLDIILREWGITTVVISGTTTENCCHATARDAMFRNYGVVFL
                     SDATATYDYPDRGFGPMANTDVHHATLVILAASTAHVMSVADMAARVEHGRRTAATSS
                     HAA"
     gene            complement(1953..>2183)
                     /locus_tag="VGC82_11600"
     CDS             complement(1953..>2183)
                     /locus_tag="VGC82_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEY0423956.1"
                     /translation="EVFQVRFVPSGEESDDIDPESDDEIPIEGNAVDLGEAAAEQLGL
                     ALPPYPRMPGVEMPEHEDEPQPHPFAALRRLN"
BASE COUNT          411 a          684 c          691 g          397 t
ORIGIN      
        1 gtcggcggcg cgctggccat gctgaaggac gggctgttcg agcgttttcc ctgcaacgcc
       61 gtctacggca tgcacaaccg ccccggcctg ccggtgggcc gcttcatcac cggccccggc
      121 gccagggcag ccggcggggc gttcttcgac atcaccgtca ccggcaaggg ggcgcacggc
      181 gcgcacccgc agcaaggtat cgatccggta ctggtagcct gccatctggg aacggctttg
      241 cagtcgatcg tgtcccgcaa tgtctccgcg atggacaccg ccgtgctgag catcacccgc
      301 atccagtccg gcgacgctta taacgtgatc ccgcaatccg ccgtcatggc cggcacggtc
      361 cggacgctga agcccgaggt gatgacgctg atcgaacaga acatgaagcg cctggtatcc
      421 agcgtcgccg cgggcttcgg cgccgaagcc ttggtcgatt tccgcgtcat cttcgcgccg
      481 atggtcaacg acgcggcgga agccatcgcc tatggcgacg ccgcggccgg actgacaggc
      541 gaggaaaacg tgcgtcgcga tgggcctcca ggcatggggt cggaggattt cagcttcatg
      601 atggagcagg ttcccggcgc gcacatcaac ctgggcaacg gcgaaagcgc ggcgctgcac
      661 aatcataaat atgactttaa cgacgaggcg atcccttacg gcgtcgcgct gtatgccgcg
      721 atcaccgaga agaagttgcc gaagttttcg cccgactgat gctgacccgg cccaacggcg
      781 gagacctgac gatctggttc gacgggcagg caatacccgc tcgaaccggc gacagcgtgg
      841 ccatggcgct gcttgccgcc ggcatcactg tcacgcggac gacgccggta tcggggcgcc
      901 gcgtggtccc tactgcatga tgggcgggtg tttcgaatgc ctggcggagg tggacggcca
      961 gcccaacgtg cagacctgca tggcgagcgt gcacgaaggc atgcgagtcc tccgccagca
     1021 ggggccgcgg tcgattcccc aagaatgact attcgcccgc gcgacaaata gccggcaacg
     1081 cggcccgttt gcttcaggca gcgtgcgagg aagtcgctgc tgtgcggcga ccatgttcca
     1141 cgcgcgcagc catatccgcg accgacatga catgcgcggt cgacgcagca agtatcacca
     1201 gagtcgcgtg atgaacgtcc gtattcgcca tggggccgaa gcctctgtcc ggatagtcgt
     1261 acgtcgccgt cgcatctgac aggaagacga cgccatagtt gcggaacatc gcatcgcgcg
     1321 cggtggcatg acagcaattc tcggtggtcg ttcccgagat gacgacggtg gtgatacccc
     1381 actcgcgcag aatgatgtcc aggtcggtac cgaagaatcc gctgtagcga tgcttcttga
     1441 tcacatgctc accaggagcc ggagccagtt ctgggtagat atccacgccc gcggtgccgt
     1501 ccaccagggc ggcgcggctg gcgatcggcg ggtgcatgtc gtcgaacaaa cccatatcgc
     1561 tgccgtcacg gcgatgcaca tgggcggtat agatcacgcg gatgcctgca tcgcggcaaa
     1621 ttgtcagcgc ttcagccagc ttgggcacca ctgcgcgggc ggccggcgtc tccatcggtg
     1681 ccccggctgc gacaaagtca ttttgcatat ccgcaacgat catggcagtc cttgtcgggt
     1741 ctatcgtatc gatcttcatc gcagcgcccc ttgttgcaag tccacttgat cgcagccagg
     1801 ataacacagc attttaatgt cgccagcaaa gaccgtccct attcttgtgg aaggtttaaa
     1861 caacgcggga ggacaagccg tatgtccagg ggccgcatat tcaggggccg catgtccagg
     1921 ggctggcgaa cctgcttcag gctggcgaac tttcagttca accggcgtaa cgcggcgaat
     1981 ggatgcggct gcggttcgtc ctcgtgttcc ggcatctcga cacccggcat cctcgggtag
     2041 ggaggcagcg ccaggccgag ttgttcggcc gccgcttcgc cgagatcgac agcattgccc
     2101 tcgatgggaa tttcatcgtc cgattccggg tcgatatcgt ccgattcttc gccggatgga
     2161 acaaagcgga cctggaaaac ctc
//
LOCUS       DASWQN010000683         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_724744, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000683 DASWQN010000000
VERSION     DASWQN010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724744"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VGC82_11605"
     CDS             complement(<1..986)
                     /locus_tag="VGC82_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY0423957.1"
                     /translation="MPIPQTMTYIAANGAGGPEVLGLATGPVPAPKPDEVLIRVQAAG
                     INRPDVAQRQGSYPPPAGASPILGLEVAGEVVATGAQVTTLSVGDQICALTNGGGYAE
                     YCTAPAPQCLPWPKGYDAIRAGALPETSFTVWANLFQIGRLSKGETALVHGGTSGIGV
                     TAIQFAREFGARIFATAGSDEKCAACLKLGADAAINYRTQDFAVEVKTLTEGRGVNVI
                     LDMVGGPYMARNVRSLGLDGRLVLIAFLQGSKVPEFDFVPVLVRRLTIAGSTMRPRTT
                     AQKGQIAAELQEKVWPVLDAGRCPPVIHATFPLAQAAEAHRLMESSTHIGKI"
     gene            1113..>2945
                     /locus_tag="VGC82_11610"
     CDS             1113..>2945
                     /locus_tag="VGC82_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEY0423958.1"
                     /translation="MPRLGLRLTPQGHLIAEDQDDAPEIESRVGTRLVEAFAQGSGYG
                     LVRLGAGEVGQALPPVFVWWRSFAARYIGAVCLNASGREDDAMIPVVAPPDAGDLRSL
                     VLTAPMMTGAEYLTEAVLLALWDETALAFAAALAASGTGLQRFLNTMNAAWNLVGRVH
                     FNLAENRRDEEQPFAFMATYTTRLSAQARAQHVPLGQALQEYAGAANRDKLLSLLLPV
                     KRAAERCEWLRPMVDAGEIYHPLRWGPAEASRFLHSLPELESAGIIVRMPASWRANRP
                     NRPRVAATVGSRKPSALGLEGVLDFRMSVILDGEPLTEQEVATLLAGTDDLVLLRGQW
                     VEIDRARFEKTMERFRAAEALAAQDGMSFTEAMRMLAGASISADDPAVTDPDWSRVTA
                     GPWLEETLTALRAGDGDGPDPGPALKGTLRPYQRAGTRWMHLLSSLGLGACLADDMGL
                     GKTIQVLALLLAQQQAGRPSLLVAPASLLGNWAAEIEKFAPGLKAVIVHPSAMSAEQI
                     KQFTPEHHDLAITSYGSLLRMPALTAASWRFVILDEAQAIKNPAARQTKAAKALQAKA
                     RIALTGTPVENHLGDLWSIFDFINPGLLGSAKQFSRYAKSLAERT"
BASE COUNT          481 a          968 c          993 g          503 t
ORIGIN      
        1 atgatcttgc cgatatgcgt gcttgactcc atcaatcggt gcgcctcggc tgcctgagcc
       61 agcggaaatg tcgcatggat caccggcgga caccgcccgg catccagcac cggccagact
      121 ttttcctgca attccgccgc gatctgccct ttctgcgccg tggtgcgggg ccgcatcgtc
      181 gagccggcga tggtcaggcg ccgtaccaac accggcacga agtcgaactc cggcactttt
      241 gatccctgca gaaacgcgat cagcaccagg cgcccatcga ggcccagcga gcgcacgttg
      301 cgtgccatgt acggcccgcc gaccatgtcc agaatcacgt tgacgccgcg gccctcggtc
      361 agcgtcttca cctcaacggc gaagtcttgc gtgcggtagt tgatcgccgc atccgctccc
      421 agtttcaggc aagcggcgca tttctcgtcc gacccagcgg tcgcgaagat ccgcgcgccg
      481 aactctcggg caaactggat ggcggtaacg ccgatgccgc tggtgccgcc gtgaaccagg
      541 gcagtctcgc ccttggataa ccggccgatc tggaacaggt tggcccaaac ggtgaaggac
      601 gtttccggca gggcgccggc ccggattgcg tcgtagcctt tcggccaagg caggcattgc
      661 ggcgccggcg cggtgcaata ctccgcgtag ccgccgccat tggtcagggc acagatctgg
      721 tcgccgacgg acaaggtcgt gacctgagcg cccgtggcga cgacctcccc cgcgacctcc
      781 aggcccagga tcgggctggc gccggcggga ggcggatacg acccttgccg ttgcgcgaca
      841 tccggccggt tgatgccggc ggcctgaacc cggatcagca cctcatcggg cttcggtgcc
      901 ggaaccggac cggtggccaa accgagaacc tcgggtcctc cggcgccgtt ggctgcgatg
      961 taggtcatgg tttgcgggat gggcatgccg ggttcctcca atcgggatct tgcaccgaat
     1021 cctaggcgcg tcgcccgccg tggcaaaggc gacaatgaat tgtcccggcc cggcccgcga
     1081 cgccgaaccc gttttccgga tacaatatcc ccatgccccg actcggatta cgcctgaccc
     1141 cgcagggcca cctcatcgcc gaggaccagg acgatgcgcc ggagatcgaa tcccgggttg
     1201 gcactcgtct ggtcgaggct ttcgcgcaag gatccggcta tggcctggtt cggcttggag
     1261 ccggtgaggt tgggcaggcc ttgccaccgg tcttcgtctg gtggcgcagc tttgccgccc
     1321 gctatatcgg cgccgtgtgc ctgaacgcat ccggacgcga ggatgacgcc atgatcccgg
     1381 tcgtggcgcc gccggatgcc ggcgatctcc ggtcgttggt gctgaccgcc ccgatgatga
     1441 caggcgccga atatttgacc gaagcggtgc tgctggcgct gtgggatgaa acggcattgg
     1501 ccttcgccgc ggctctcgcc gcttccggca ccgggctgca gcgcttcctg aacacgatga
     1561 acgcggcctg gaacctggtc ggccgcgtgc atttcaatct ggcggaaaat cgccgcgacg
     1621 aggaacagcc gttcgcgttc atggcgacct acacgacgcg cctgtcggcg caggcccggg
     1681 cgcaacacgt cccgctgggc caagcgctgc aggaatatgc cggggcggcc aaccgggaca
     1741 aattgttgtc gctgctgctg ccggtgaagc gcgccgccga acggtgcgag tggctccgcc
     1801 cgatggtcga tgccggggag atttatcatc cgctgcgctg gggaccagcc gaggcgtcgc
     1861 gtttcctcca cagcctgcct gaactggaaa gtgccggcat catcgtgcgc atgccggcct
     1921 cgtggcgagc caaccggccg aaccgcccgc gggtcgcggc cactgtcggc agccgcaaac
     1981 cgtctgcgct ggggctggaa ggcgtgctcg acttccgcat gagcgtgatc ctggacggcg
     2041 aaccgttgac cgagcaggag gtcgcgacgc tgctggcggg caccgacgat cttgttttgc
     2101 tgcgtggcca gtgggtggag atcgaccggg cgcgttttga gaagacgatg gagcggttcc
     2161 gcgcggccga ggcgctggcg gcgcaggatg ggatgagctt taccgaggcg atgcggatgc
     2221 tggccggcgc ctcgatcagc gcggacgatc cggcggtgac cgatcccgac tggtctcgtg
     2281 tcaccgccgg cccatggctg gaggaaacat tgacggcgct gcgggctggt gacggcgacg
     2341 gacccgatcc tggacctgca ctgaagggaa cgttgcggcc ttaccaacgt gccggcacgc
     2401 ggtggatgca tttgctgtcc agcctcggcc ttggtgcctg tctggccgac gacatgggcc
     2461 tggggaagac gatccaggtg ctggccttgc tgctggcaca gcagcaggcc gggcggccaa
     2521 gcctgctggt cgcaccggcg tccttgctgg gcaactgggc ggcggagatc gagaaattcg
     2581 cccccggcct caaggccgtc atcgtgcatc cgtcggcgat gagcgcggag cagatcaagc
     2641 agttcacgcc ggagcaccac gatctggcaa tcaccagcta tggctcgttg ctgcgaatgc
     2701 cggcgctgac ggcggcttcg tggcgctttg tcatcctcga cgaggcccag gcgatcaaga
     2761 accctgccgc caggcagacc aaggccgcga aggcgctgca agccaaggcg cggatcgctt
     2821 tgaccggaac gccggtggag aaccacctcg gcgacctgtg gtcgatcttc gacttcatca
     2881 accccggcct gctgggcagc gccaagcagt tctcccgcta tgccaagagt ttggccgaac
     2941 ggacc
//
LOCUS       DASWQN010000684         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_198199, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000684 DASWQN010000000
VERSION     DASWQN010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198199"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /locus_tag="VGC82_11615"
     CDS             complement(<1..1767)
                     /locus_tag="VGC82_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY0423959.1"
                     /translation="MQTVISLLPYLWPVGNPGARIRVVAALIFMALSKVATVYVPIIY
                     GRIIDALAPHDAAATAFIVPVALIMAYGAVRIGGAGFGEVRDALFASVQQRAVRLLAL
                     RTFRHLHAVSLRFHLDRQTGGMSRVIDRGVLGMQSVLRLAVFNVVPTALELVMVTAII
                     WHMFDWRYAAVTCLAVLVYVSFTAVLAGRRGRYRRTMNDTDNDASTKSLDSLINYETV
                     KYFGNEEHEAQRYDGALARYERAAVRVQVSLNMLNLGQAAIIAIGLTLIMLLAASGMR
                     DGSMTVGKFVVVNTYLIQLYQPLNFLGMVYMTIKQGLVDMEQMFALLRVQREITDKPG
                     AKALTAHLSESSAGEVVFENVRFGYQPNRTILQGIDFSVPAGGKLAIVGPTGAGKSTI
                     NRLLFRFYDVTGGRILIDGQDIRDVTQDSLRAAIGVVPQDTVLFNDSIRYNIGYGRPG
                     ASDADIEHAAKLAQVHDFVLRLPEGYDTRVGERGLKLSGGEKQRVAIARTILKDPRIL
                     VLDEATSALDSRTEQDIQTALATVSLRRTTLVIAHRLSTVVDADEIIVLQDGLVAERG
                     KHSALLALGGLYARMWALQATEH"
     gene            1882..2730
                     /locus_tag="VGC82_11620"
     CDS             1882..2730
                     /locus_tag="VGC82_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4743 domain-containing protein"
                     /protein_id="HEY0423960.1"
                     /translation="MDQYAHTGFERHVRACNNAEIPGSRIPFLIHEQVVGWVPPRIAS
                     ALQAMASVKCASDRVVLDDPAALPGIARVLSERGLFRWRGEAFDVRARPDAPALTQID
                     RGAIPSFGIQAAGVHVNGLVQRSDGLHFWVARRASDKILDPGKLDHIVAGGVPAGLDP
                     AATLIKEAGEEAAIPPALAEAAVQVGVISYAMDRPEGLRRDWLYCYDLVLPNDFTPQA
                     ADGEVEAFQLWPIARVVEAVRETDDFKFNVNLVLIDLFLRQELIPDPEARTLRASLQA
                     AYASMR"
BASE COUNT          517 a          848 c          965 g          515 t
ORIGIN      
        1 atgttcggtc gcctgtagtg cccacatcct ggcatacagg ccaccgagag ccagcaaggc
       61 actgtgtttg ccgcgttcgg cgaccaaacc atcctgcagc acgatgatct cgtcggcgtc
      121 gacgacggtg gacaggcgat gggcgatcac cagggtggta cggcgaaggg agacggtagc
      181 gagtgctgtc tggatgtcct gttcggtgcg gctgtccagc gcgctggtgg cctcatccag
      241 gaccaggatg cgggggtcct tcaggatggt gcgagcaatg gcgacgcgct gcttttcgcc
      301 gccggacagt ttcaggccac gctccccgac gcgggtatcg tagccttcgg gcaggcggag
      361 gacgaaatcg tggacctggg cgagctttgc cgcgtgttcg atgtcggcgt cggaggcgcc
      421 tggccggccg tagccgatgt tgtagcgaat gctgtcgttg aacagcacgg tatcctgcgg
      481 caccacgccg atggccgcgc gcaggctgtc ctgcgtcacg tcccggatat cctggccgtc
      541 gatcaggatg cggcctccgg tcacgtcgta gaagcggaac agcaagcggt tgatggtcga
      601 tttaccggca ccggtggggc cgacgatcgc cagcttgccg cccgccggta ccgaaaagtc
      661 gatgccctgc aatatcgtcc ggttgggctg gtagccaaag cggacgtttt cgaacacgac
      721 ctccccagcg gagctttcgg aaagatgggc ggtcaaagcc ttggcgccgg gtttgtcggt
      781 gatttcccgc tggacgcgga gcagcgcgaa catctgttcc atgtccacca gaccctgctt
      841 gatcgtcata tagaccatgc caaggaagtt cagcggctga taaagctgga tgaggtaggt
      901 gttgaccacg acgaacttgc cgacggtcat gctgccgtcg cgcatgccgg atgccgcgag
      961 cagcatgatc agggtcaggc cgatggcgat gatcgccgcc tggccaaggt tcagcatatt
     1021 cagcgagacc tggacgcgga ccgcggcgcg ctcgtagcgg gctagtgccc cgtcatagcg
     1081 ctgtgcctcg tgttcttcgt tgccaaagta ctttaccgtt tcgtagttga tcaggctgtc
     1141 cagcgacttc gtcgaggcat cgttgtcggt atcgttcatc gtccggcggt agcggccgcg
     1201 gcggccggcg agcaccgcgg tgaacgagac atagacgagc accgcgaggc aggtgacggc
     1261 ggcatagcgc cagtcgaaca tgtgccagat gatcgcagtg accatcacca gttccagcgc
     1321 cgtgggaacg acgttgaaaa ccgcgaggcg aagcaccgac tgcatcccga gaacgcctcg
     1381 gtcgatgacc ctggacatgc cgccggtctg gcgatcgagg tgaaaacgca ggctgacggc
     1441 gtgcaggtga cggaatgtac gcagtgcaag cagacgcacc gccctttgct ggaccgaggc
     1501 gaacagggcg tcacggacct cgccaaagcc ggccccgccg atccgaaccg cgccgtacgc
     1561 catgatcagg gcgaccggga cgatgaacgc cgtggccgcg gcgtcatgcg gtgccaaggc
     1621 gtcgataatg cggccgtaga tgatcggaac gtaaacggtg gccaccttgg acagcgccat
     1681 gaagatcaat gccgcgacga cgcggatacg cgcgcccgga ttgccgaccg gccacaggta
     1741 gggcagcagg gagatcacag tctgcatggt ggatcgcggc gcccgctggg atggcggcga
     1801 gtccgccgcg gcttgtgggt ggctgcttcg tctcatgctc ggcatgtggc atgatgctgc
     1861 gtggtttgaa aggcaaagag cttggatcag tatgcacaca ccggctttga gcgtcatgtg
     1921 cgtgcctgca acaacgcgga aattcccggc agccgcatac cgttcctgat ccatgagcag
     1981 gtggtcgggt gggtaccgcc tcgcatcgcc tcggcattgc aggcgatggc atcggtgaaa
     2041 tgcgcgagcg accgggtggt gctggacgat cctgcggcgc tgcccggcat cgcccgggtg
     2101 ttgtcggagc ggggattgtt tcgctggcgc ggagaggcgt tcgacgtgcg tgcgcggcct
     2161 gatgcaccgg cgctgacgca gatcgaccgt ggggcaatcc catcctttgg gatccaggcg
     2221 gccggagtcc acgtcaatgg actggtccag cgctcggatg gccttcattt ctgggtcgct
     2281 cgccgagcct ccgacaagat cctggacccg ggcaagctgg accatatcgt tgccggcggc
     2341 gttcccgcgg gattggatcc agcggcgacg ctgatcaagg aggcgggtga agaagccgcg
     2401 attccgcccg cattggcgga ggcggcggta caggttgggg ttatctcgta tgcgatggac
     2461 cgccccgaag ggttacggcg cgactggctg tactgctacg acctggtgct gccgaacgac
     2521 ttcacgccgc aggcggccga tggcgaagtc gaagcgttcc aactctggcc gatcgcacgt
     2581 gtcgtcgaag ccgtccgtga gaccgacgac ttcaagttca acgtcaacct ggtgctgata
     2641 gacctgttcc tccgccagga actgatcccg gatccggaag ccagaacgct tcgcgcaagc
     2701 ttgcaagcgg cctatgcttc catgcgttaa accgggtgct tcccgaaacc cgccgttcat
     2761 cggcgcatgg tccacgaaga aagcgcttca acgaaggctt ccaccgcctg cgggccggcg
     2821 ataatcgtca ggcgcttcgg gctgc
//
LOCUS       DASWQN010000685         4372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1121184, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000685 DASWQN010000000
VERSION     DASWQN010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121184"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VGC82_11625"
     CDS             <1..326
                     /locus_tag="VGC82_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423961.1"
                     /translation="SGGLVGSLTGAGVSREEAHVYAEGVKRGGTLVTVRANETDASRI
                     EAILARAGSTDWQQRRTAYGSDWKGFDDASSGLRGNESTAPTASLPGETAAGYSPATD
                     PNRRL"
     gene            533..1807
                     /locus_tag="VGC82_11630"
     CDS             533..1807
                     /locus_tag="VGC82_11630"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0423962.1"
                     /translation="MGALFAQETDAAAAKPDSLNVGRIDRITQFFGDEVASHRLSGAV
                     VMISRGGNIAYQEAFGVRDPDTRAGMLRDSIFRIYSMTKPVTAVAVLMLVEDGKIHLG
                     DAASAYLPAMKSPRVLIEQTDGVGGRFATTVPADHEITIEELLTHTSGITYGAGSSLA
                     EQMMKDAGIGLELGAAGIALASRINDAQMVEKIARVPLMFQPGTAWHYGRSFDVLLAL
                     IEAVSGVTADQFYEQRIFRPLGMTDTFFNLPPDRRNRVAQPGPNPYSFFGRVPLLTNV
                     SLPRTFLAGGAGLLSTAPDYMRFASMLANEGELDGVRILMPETVALMTSDRIGPALAR
                     PPAFNPAEGYGFGLGVPVRTRSTASIPGEVGEFNGYGAAGTLFWVDPAEHLVAVLMTQ
                     SPDWADRKRAVFRTLVYQSLPNNRSRSSSAAH"
     gene            1936..2820
                     /locus_tag="VGC82_11635"
     CDS             1936..2820
                     /locus_tag="VGC82_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY0423963.1"
                     /translation="MRYRVKHTTRYAYGTPVELAAHMVHLRPRRRSWQTVISERIVTD
                     PAPARRRDGLDHFGNHVTWLFMDMPHADFEVTAYSTVEVDCPPPPAVSDTPAWETVVD
                     ATRGPEGWEAVEFRFGTKMAPLDPASEAYAAQSFTKGRPVLDALLELNERFYTEFRFR
                     SGVTSISTPVSQVMARREGVCQDFSHAMVSGLRGIGIPARYTSGYIRTRPPPGQVKRQ
                     GSDQSHAWVGCWLGPQFGWVDVDPTNGIVVKDEHVLLGWGRDFSDVSPVRGVILGGGD
                     HALSVGVDLEPVDNGEPE"
     gene            2817..3497
                     /locus_tag="VGC82_11640"
     CDS             2817..3497
                     /locus_tag="VGC82_11640"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0423964.1"
                     /translation="MTSLRVLSTLAVMGAMRELGAQYEAQTGISVEADFSPTVALLER
                     LRGGETADIAILTAQGIDDLIHEGIVRNATRTNMALSFVGIAVKAGAAKPDIGSVEAL
                     KATLLAARSVAYSKIGASGIFFAGLLERLGIAAEVRASIVPSGFTAERLVSGEAELAV
                     QQVSELMMVPGIEVVGPLPPEVQTVATFSGGLLAASPRPEEASALLRFLASPEVAPIL
                     RRSGLEPA"
     gene            complement(3573..>4372)
                     /locus_tag="VGC82_11645"
     CDS             complement(3573..>4372)
                     /locus_tag="VGC82_11645"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY0423965.1"
                     /translation="TGGTISIFSLFLAGVMPGLLLGFSLIILCLVMAYRNGHPKGDAV
                     PVRLAGKMALEAFWGLITLIIILGGILGGIFTAVEAGAVACVWAFFVTMFIYRDYRWG
                     DLPALVHRTLRTVAMVMTLIAFASSVGYMMALMQVPAKITGFLLTFSNNKYVILMMIN
                     VLLLVLGCLLDMAPAILIVTPILLPVMINFGVDPVHFGMIMLMNLGIGLCHPPVGAIL
                     FVGCAVGKVTLEQVMREIWPFYLVMFTVLMLVTYVPSISLWLVHLHG"
BASE COUNT          813 a         1419 c         1357 g          783 t
ORIGIN      
        1 cgtcaggcgg cttggtcggt tcgttgaccg gtgccggagt cagccgtgag gaggcccatg
       61 tttacgccga aggcgtgaaa cgtggcggta cattggtcac cgtccgagcc aacgagacag
      121 acgcttcgcg gatcgaggcg attctggcgc gggccggttc gaccgactgg caacagcggc
      181 gcaccgcgta cggttccgac tggaagggct tcgacgacgc gtcgagcggg ctgcgtggta
      241 acgaatccac tgctccaacg gcttcgctgc cgggcgagac cgcggccggc tattcgcctg
      301 caaccgatcc caaccgccgg ctttgacggc atgatagaga acgtctgggg gaacaccccg
      361 gacgttctcc gtttcgcttc cggccccagc gtgcgattgc cggaccataa ggagtgtgca
      421 aacttcggcg gcagcgcttg gcgaggcagc ccgccgcccg attttgcttc ataatgatca
      481 caggtagtac cggagagaaa tcgtcaaacg ccgccggctt atcatggctc ccgtgggcgc
      541 ccttttcgcg caagaaaccg acgcggcggc tgcgaaaccc gacagcctaa acgtcgggcg
      601 gattgatcgt atcacccagt ttttcggcga tgaggtcgcc agccaccggc tgtcgggcgc
      661 agtggtgatg atcagccggg gtgggaatat cgcctaccag gaagcgtttg gcgttcgtga
      721 ccccgacacc cgagcgggca tgctgcgcga cagtatcttt cgcatctatt cgatgaccaa
      781 gccggttacc gccgttgccg tgctgatgct ggtggaagat ggcaagatcc acctcggcga
      841 tgccgcatcg gcctatcttc cagcaatgaa aagcccgcgc gtgctgatcg agcaaactga
      901 cggcgtcggc ggtcgcttcg ccaccactgt gcccgcggat catgagatca ccatcgagga
      961 gttgctgacg cacacctcgg gaatcaccta tggcgccgga agttcacttg ccgaacagat
     1021 gatgaaggat gccggcatcg gcctcgagct tggcgccgcc ggcatcgcgc tcgccagccg
     1081 gatcaacgac gctcagatgg tcgagaaaat cgccagggtg ccgctgatgt tccagccagg
     1141 gacggcgtgg cactatggcc gctcgttcga cgtgctgctg gccctgatcg aggccgtctc
     1201 cggcgtgacc gctgaccagt tctacgagca acggattttc cgcccgctcg gcatgaccga
     1261 cacgttcttc aatttgccgc cggaccggcg aaaccgcgtc gctcagccgg gcccaaatcc
     1321 atattcgttt ttcggcaggg tcccgctgct gaccaacgtg tcgctgcctc gcactttcct
     1381 ggcaggcggg gccgggttgc tgtcgaccgc accggactac atgcgcttcg cctcgatgct
     1441 ggcgaatgaa ggtgaacttg atggggtccg gatactgatg cccgaaaccg tggccttgat
     1501 gacgtcggat cgcattggtc cggcacttgc caggccgccc gcgttcaacc cagccgaagg
     1561 ttacggcttt ggcttgggcg tgccagtgcg aacaagatca accgcgtcga tccccggcga
     1621 ggtgggcgag ttcaacggct acggcgccgc cggaacgctg ttttgggttg atccggcgga
     1681 acatcttgtc gctgttctga tgacccagtc gccggattgg gcggatcgta agcgagcggt
     1741 atttcgaacg ctcgtctatc agtcgttgcc gaataatcga tctcggtcgt cctcagcggc
     1801 acattgacct tgtggtcaac gatcgtgtgc tcaacggccc gcttgttccc ggatggtgcg
     1861 gcgggacggg ataccgggtc ctgattattg accccgtccc gagacaaacg ccttcgcaca
     1921 cgataaacac cgccgatgcg ttaccgcgtg aagcacacta cccgatacgc ctacggcacg
     1981 ccggtcgagc tcgccgcgca catggtgcat ctgcgcccgc ggcggcgatc gtggcagacc
     2041 gtcatctcgg agcgtatcgt gaccgaccca gcccctgcac gcaggcgtga cgggctggac
     2101 catttcggca accatgtcac atggctgttc atggacatgc cgcacgccga tttcgaggtt
     2161 accgcctatt ccaccgtcga ggtcgattgt cccccgcctc cggccgtcag cgacacgccc
     2221 gcctgggaaa ccgtggtgga cgcgacccgc ggtcccgagg gctgggaagc cgtggaattc
     2281 cggttcggga ccaagatggc accgctcgac ccggcgtccg aagcctacgc cgctcagtca
     2341 ttcaccaagg ggcgcccggt cttggacgcc ctgctggagc tcaacgaaag attctacacc
     2401 gaattccgct ttcgctccgg cgtcacctcc atctccacgc cggtatccca ggtcatggcg
     2461 cgccgggagg gcgtatgcca ggatttctcc cacgccatgg tcagcggcct gcgcgggatt
     2521 ggaataccgg cgcgctatac ctccggctat atccgcaccc gaccgccacc gggtcaggtc
     2581 aaacgccaag gctccgatca atcgcatgcc tgggtcggct gctggcttgg accccagttc
     2641 gggtgggtgg acgtggaccc gaccaacggc atcgtggtga aggacgaaca cgtcctgctc
     2701 ggctggggcc gggacttcag cgatgtcagc cctgttcgcg gcgtgatcct ggggggcgga
     2761 gaccatgccc tttcagttgg cgtcgatctt gagcccgttg ataacggaga accggaatga
     2821 ccagccttcg ggtgctttcg acccttgccg tcatgggcgc gatgcgcgaa ctgggcgccc
     2881 aatatgaagc ccaaacgggc atttcggtcg aagcggattt ctcccctact gtcgccctgc
     2941 tcgagcggct gcgcggcggc gaaacggccg atatcgccat cctgaccgcc cagggcatcg
     3001 acgacctgat ccatgaaggc atcgtgcgga acgcgacccg caccaacatg gccctgtcgt
     3061 tcgtcggcat cgctgtcaag gccggcgccg ccaaaccgga catcggatcg gtggaggccc
     3121 tgaaagccac cctgctcgcc gcgcgctccg ttgcctactc gaagatcggc gccagcggca
     3181 tcttcttcgc tggcctgctg gagcgcctgg gcatcgcggc cgaggtcagg gcgagtatcg
     3241 tcccctccgg cttcacagcg gaacgcctgg tcagcggtga ggccgaactc gcggtgcagc
     3301 aggtcagcga actgatgatg gtcccgggca tcgaggtcgt cggtccgctg ccgccggaag
     3361 tccagaccgt cgccactttt tccggcgggc ttctggccgc gtcgccccgg ccggaggaag
     3421 catccgcgct gctgcggttc cttgcatcgc ccgaggtggc cccgatcctg cggcgttccg
     3481 gcctggagcc ggcctgaggg tacccgccgc cgcggaaagg ccggcttctc gtttcgcgcc
     3541 tcgaccctcg tcaagggcgc gaaacgcgct gctcagccgt gcagatgcac cagccatagg
     3601 gaaatcgagg gaacgtaggt caccagcatc agaacggtga acatgaccag gtagaatggc
     3661 cagatttccc gcatgacctg ctccagcgtc acctttccca cggcgcaccc cacgaagagg
     3721 atcgcgccga ccggcgggtg gcatagcccg atccccaggt tcatcagcat gatcatgccg
     3781 aaatgtaccg gatcgacccc gaaattgatc atcaccggca gcaggatcgg cgtgacgatc
     3841 aggattgccg gtgccatgtc caaaaggcat cccaacacca gcaacaacac gttgatcatc
     3901 atcaggatga cgtatttgtt gttcgaaaac gtcagcagga agccggtgat cttcgccggc
     3961 acctgcatca acgccatcat gtagccaacc gaggacgcga acgcgatcag cgtcatcacc
     4021 atcgccaccg tgcgcaacgt ccggtgtacc agggccggca ggtcgcccca gcggtagtcg
     4081 cggtagatga acatggtgac gaagaacgcc cagacgcagg caaccgcgcc ggcctcgacc
     4141 gcggtgaata tgccgcccag gataccgccg aggataatga tcagcgtgat cagtccccag
     4201 aacgcctcca gtgccatctt tcccgccagg cgcacgggca ccgcatcgcc tttcggatga
     4261 ccgttgcggt acgccatcac cagacacagg ataatcagcg aaaaaccaag cagaaggccg
     4321 ggcatcaccc ctgcgaggaa aaggctgaag atcgagatcg ttccgccggt gg
//
LOCUS       DASWQN010000686         6246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_858453, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000686 DASWQN010000000
VERSION     DASWQN010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858453"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VGC82_11650"
     CDS             complement(<1..880)
                     /locus_tag="VGC82_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PotD/PotF family extracellular solute-binding
                     protein"
                     /protein_id="HEY0423966.1"
                     /translation="MATTRSRRFGRRSLLATAGIAAGSGAVTGFPTIWAQNIKNVTLR
                     QFGTGVSNLNAVADKVKADLGFTLSMTALDTDAVTQKAVTQPKSYDIADIEYFAIKKV
                     FPTGVMQPMDAKKIKYADKIVPIFTKGKLTPESAMAQGTTPYSVGFVDGPNGKIFAKG
                     QTEWMTLIPTIYNADTLGIRPDLVGRPITHWKDLVDPAFKGKASILNIPSIGIMDAAM
                     VMESLGTLKYADKGNMTKPEIDKTVAFLIEAKKAGQFRAFWKSFDESVNLMASGEVII
                     QSMWSPAVAAVRSKGIA"
     gene            complement(963..1910)
                     /locus_tag="VGC82_11655"
     CDS             complement(963..1910)
                     /locus_tag="VGC82_11655"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0423967.1"
                     /translation="MRSNAIDVRPAGGAVGAEITGIDLSQPLAGSVRNTIRKTLADRG
                     VVFFRDQALTPEQHIGLARQFGDININRFFAHADGYPEIALVVKEPEQTRNIGGGWHT
                     DHSYDKAPALGSILYAREVPPSGGDTLFASMYAAYDALSDGLKQTLAGLRAVHSSRHV
                     FGVERGDLKGRIGNAVAAVQDAVHPVVITHPDSGRKTLYVNPGFTLRFEGWTDDESRP
                     LLEFLYRHAAKPEFGCRFVWRNGSIAFWDNRATWHYAVNDYQGERRLSPPDHGRGVRT
                     AGLIGRRPGGVWRSCPSAWSHAPHGAPKRSVACNLISRD"
     gene            2050..2532
                     /locus_tag="VGC82_11660"
     CDS             2050..2532
                     /locus_tag="VGC82_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEY0423968.1"
                     /translation="MNPIAWLISLALPACGFPAAEGLPVPLPMDTAHIVRPATPNTSL
                     AAPEGFSPQPDIVTPSYRLPPERLFALVQEVAASQPNTYRAALFSDRLQVHYVARSAM
                     FNFPDLVMVQVGQTGADSSNIILYSRSVYGRSDFGANRKRVEAWLAALRAKLPPSSER
                     "
     gene            2540..4315
                     /locus_tag="VGC82_11665"
     CDS             2540..4315
                     /locus_tag="VGC82_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY0423969.1"
                     /translation="MRGIGPFLKDAWQLARPYFMTSEERWSARGLLVAIIVMNLTLVG
                     LTVVLSFWRREFYNALQDKDWVAFLELLFLYRNTPSGLLPGFCEVAGIHIVLAVYSVY
                     LNQMLQIRWRRWLTRQFLTEWLADRAYYNISLTTDRAAVGTDNPDQRVAEDLRDFCQM
                     SLTLTLDLLSNIVSLFSFVSILWGLSGAIEAFGIPIPGYMVWVALAYALVGTTLTHLV
                     GRPLSILNFRQQRVEADFRYALVRVRENMESIALYGGEQEEGVTLRERFAAVIGNWYQ
                     IMRRTKMLNSLINGYLQVASVFPFVVAAPRYFSGAIQLGGLMQTVGAFGSVQSSMSWF
                     VESYSEIARWRAIVERLATFHRAIVKARAETHGGFVNTVGTAGTVRLRDVTMSLPDGT
                     KLLDGADLLLTAGHSVVITGRTGSGKSTLFRVLAGIWPFGQGQVELPPKSFFLPQQPY
                     IPLGTLRHVITYPNAVDAFSEQEIEQALTDAGLPDLCPRIEKDDNWPQRLSGGEQQRV
                     ALARALLAKPDWIFMDEATASLDPDAEEALYRVLKQRLPNATLVSIAHRPSVAAFHDT
                     RLVFKREGAGAGSLTPVAAVPAVGE"
     gene            4317..5993
                     /locus_tag="VGC82_11670"
     CDS             4317..5993
                     /locus_tag="VGC82_11670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEY0423970.1"
                     /translation="MTMPRFSEISEPRPTQAGLAEAYVTIDAAIDRGDHATAMADWDA
                     LRRHYDTWSALVHLRFAQDTTDAQAKADRDYADALAPEAAELETGLKRRLLADSNRQA
                     VAALVGEHAPRLWETDVTTFDPVIKPDLEEEARLSARYTELLASAKLEIAGQTVNLAG
                     LGPYAENPDRVVRHQAEQTRWRFFEANAATLDDLYDQLVRLRHGMARKLGFQTYTPLG
                     YRRLRRVDYGPDDVARYREQVVRHVVPLVGRLLEARRVENGWDRLRYWDEALVDPAGN
                     PKPAGDHDRLVDAAAAMFDGMDDKLASFYRMMSQGGFMDLKNRPGKAGGGFCTSFPTE
                     GVPFIFANFNGTHHDIGVFTHEMGHAFQNWESRNQPNVDYLWPTMEAAEINSMGLEFL
                     SYPGIGRLVGEDAADRFRRMHLIGALAFLPYGVCVDHFQHEVYAKPEASPAERHAMWS
                     RLEKIYMPWTDYGDLDFLVKGGRWQAKPHIFGSPFYYIDYTLAQCCAMQFWVRSRRDY
                     AGTVRDYVALCARGGSAPFQDLVRSAGLVSPFADGALADVVTEAAAVLKV"
BASE COUNT         1096 a         1992 c         1920 g         1238 t
ORIGIN      
        1 aggctattcc cttcgatcgc acggccgcca cggccggcga ccacatcgac tggatgatga
       61 cctcgccgga ggccatcagg ttgacgctct cgtcgaagct tttccagaac gcacggaact
      121 ggccggcctt tttcgcctcg atcaggaagg caaccgtctt gtcgatctcc ggcttcgtca
      181 tgttgccttt gtcggcgtat ttcagggtgc ccagagactc catgaccatg gcggcgtcca
      241 tgatgccgat ggacgggatg ttgaggatcg atgccttgcc cttgaaggcg ggatcgacga
      301 ggtccttcca atgagtgatt ggccggccga ccaggtcggg gcggatgccc agcgtgtcgg
      361 cattgtagat cgtggggatc agcgtcatcc actcggtctg gcccttggcg aaaatcttgc
      421 cgttcgggcc atcgacgaaa ccgaccgagt agggcgtcgt gccttgcgcc atggcacttt
      481 ccggcgtcag ctttcccttg gtgaagatcg gcacgatctt gtcggcgtac ttgatcttct
      541 tggcgtccat cggctgcatc acgccggtgg ggaagacctt cttgatcgcg aaatactcga
      601 tgtccgcgat atcgtaggat ttgggctggg tcaccgcctt ctgcgtcacc gcatcggtgt
      661 ccagtgccgt catcgacagc gtgaagccca gatccgcctt caccttgtcg gcgaccgcgt
      721 tgaggttcga cacgccggtg ccgaactggc gcagcgtgac gttcttgatg ttctgcgccc
      781 aaattgtcgg aaacccggtg acagcgccgc tgccggccgc gatcccggcc gtggcgagaa
      841 gggaacggcg gccgaaccgg cgtgatcgag tggttgccat cggcttgctg cgctccccta
      901 cgttgttcgt ttgcgatctt gcatggaccg tgccatcggc gatcggcgga aaaggccggc
      961 gatcagtcgc gactgatcaa attgcaagca acggagcgtt tcggtgcacc gtgaggagca
     1021 tgcgaccagg cggacgggca tgatcgccat acgccgcccg gtcgcctccc gatcaagcct
     1081 gcagttcgca cccctcgacc gtgatccgga ggcgacaacc ggcgttctcc ctggtaatcg
     1141 ttgacggcgt agtgccacgt cgcccgattg tcccagaacg cgatcgaccc gttccgccac
     1201 acgaaccggc aaccaaactc cggcttcgcc gcgtggcggt acaggaactc cagcagcggc
     1261 cgggactcgt cgtccgtcca gccttcgaag cgcagggtga acccggggtt cacatacagc
     1321 gtcttgcggc cactgtccgg gtgcgtgatg accacaggat gcaccgcatc ctggacagcc
     1381 gccacggcat tgccgatgcg gcctttcagg tcgccgcgct cgacgccgaa cacgtgccgg
     1441 cttgaatgca ccgcgcgcag tccagccagc gtctgcttca atccatccga cagcgcgtca
     1501 taggctgcgt acatgctggc aaacagcgtg tcgccgccgg aaggcggcac ctcgcgggcg
     1561 tataggatgg aaccgagcgc cggcgccttg tcgtagctgt ggtcggtgtg ccatccgccg
     1621 ccgatattgc gggtctgctc gggctctttc accaccagcg caatttccgg ataaccatcg
     1681 gcatgagcga agaagcggtt gatattgatg tcgccaaact gccgcgccag gccgatatgc
     1741 tgttccggcg tcagcgcctg gtcgcgaaag aacaccacgc ctcgatcggc aagcgtcttg
     1801 cggatcgtgt tccgaaccga accggcgagt ggttgggaca ggtcgatgcc cgtgatctcg
     1861 gcgcccaccg cgccaccggc tggtctgacg tcgatcgcgt tgcttcgcat gttgtcctcc
     1921 gtgttgggta catagtggca gggatgcgtt ccattgccca gcaaagtacc tctgcccagc
     1981 cttcgcaatt gacttgccgc ctgccgcctt tcggggcatc accggccaga tgatgggtgg
     2041 atgcgccgga tgaacccgat tgcctggctg atcagtctgg cccttcccgc ttgcggtttc
     2101 cccgcggcgg agggactgcc ggtgccgtta ccaatggaca ccgcgcacat cgtccgaccc
     2161 gccacgccga acacctcgct ggccgcgccg gaaggcttca gtccgcaacc cgacatcgtc
     2221 acgccgtcct accgcctgcc gcccgagcgg ctttttgccc tggttcagga ggtcgccgcc
     2281 agccagccga acacgtatcg ggccgcgctg ttttcggacc ggcttcaggt ccactacgtt
     2341 gcccgcagcg caatgttcaa cttccccgac ctcgtgatgg tgcaggtagg acaaaccggc
     2401 gcggacagca gcaacatcat tctgtactcc cgcagcgttt atggccgctc cgacttcggc
     2461 gcgaaccgca agcgggtgga agcctggctt gccgccctgc gggcaaaact cccaccatcc
     2521 agcgagaggt gacccgttca tgcgtggtat cggccccttc ctgaaagacg cctggcaact
     2581 cgctcgtccc tatttcatga cctcggagga acggtggtcc gcccgcggcc tgctggtcgc
     2641 catcatcgtc atgaacctga ccctggtcgg attgaccgtg gtgctcagct tctggcggcg
     2701 tgagttctac aatgcgctgc aggacaagga ctgggttgca ttcctggaac tgctgttcct
     2761 gtaccggaac accccaagcg ggctgctgcc cggcttttgt gaggttgccg gtattcacat
     2821 cgtactggcg gtttactcgg tctatctgaa ccagatgctg cagatccgtt ggcgccgctg
     2881 gctgacccgg caattcctga cagaatggct ggccgaccgc gcctattaca atatcagcct
     2941 gactacggat cgggcggccg tcggcaccga caaccccgac cagcgcgtgg ccgaggattt
     3001 gcgcgacttc tgccaaatgt ccctgacgct taccctggac ttgctctcca acatcgtctc
     3061 gctttttagc ttcgtcagta ttctctgggg cctgtccggc gccatcgagg cattcggtat
     3121 cccgatcccc ggctacatgg tttgggtcgc attggcttat gccctggtcg gcaccacgct
     3181 gacccacctg gttggccgcc cgctttcgat cctgaacttc cgtcagcagc gggtcgaggc
     3241 ggatttccgc tatgcgctgg tgcgtgttcg cgaaaacatg gagagcatcg ccctgtacgg
     3301 cggcgagcaa gaagaaggcg tgaccctgcg cgaacgcttc gccgcggtca tcggcaactg
     3361 gtaccagatc atgcggcgaa cgaagatgct gaattcgctg atcaacggct atttgcaggt
     3421 tgccagcgtg tttcccttcg ttgtcgccgc gcctcgctat ttctccggcg ccatccagct
     3481 tggcggattg atgcagacgg tgggggcatt tgggtcggtg caaagctcga tgtcgtggtt
     3541 cgtggagtcg tattctgaaa tcgcgcggtg gcgagccatc gtcgaacgtc tggctacttt
     3601 ccatcgagcc atcgtcaaag cgcgggcgga aacccatggt ggcttcgtga acaccgtcgg
     3661 aaccgcggga acggttcggc tgcgcgacgt gacaatgagc ctgcccgatg gcacgaagct
     3721 gctcgacggt gccgatctac ttctgaccgc gggtcattcc gtcgtcatca ccggtcgcac
     3781 cggatccggc aaatcgacat tgttccgcgt cctggccggg atctggcctt tcgggcaagg
     3841 tcaggtcgag ctgccgccga aatcgttctt cctgccgcag cagccgtaca ttccgctcgg
     3901 caccttgcgg cacgtcatca cctaccccaa tgcggtggac gcattttccg aacaggagat
     3961 cgagcaggcg ctgacggatg ccggcctgcc ggacctttgc ccgcggatcg agaaagatga
     4021 caactggccg caacgccttt cgggcgggga gcagcaacgg gtcgccctcg ccagggcgtt
     4081 gttggcgaaa ccggattgga tcttcatgga tgaggcaacc gccagcctgg atcctgacgc
     4141 cgaggaggcg ctgtatcgcg ttctgaagca gcggctgccc aacgccacgc tggtctcgat
     4201 cgcgcatcgt ccttcggtcg cggcgttcca cgatacccgt cttgtgttca aacgtgaggg
     4261 cgccggcgcc ggtagcttga cgccagtcgc cgccgtgcca gcggtaggtg agtgatatga
     4321 cgatgccgcg cttcagcgaa atttcagaac ctcgcccgac ccaagccggg ctcgccgagg
     4381 catatgtgac gatagacgca gccattgacc gcggcgatca cgccacggcc atggccgatt
     4441 gggacgcgtt gcggcgacac tacgacacat ggtcggcgct ggtgcacttg cgctttgccc
     4501 aggacaccac ggatgcacag gcaaaagcgg atcgcgatta tgccgacgcg ctggctccgg
     4561 aagccgccga actggaaacc ggcctgaagc ggcggttgct cgccgatagc aaccggcaag
     4621 ccgttgccgc ccttgttggc gagcacgccc cgcggctttg ggaaaccgac gtcacgacgt
     4681 tcgaccccgt catcaagccc gatcttgaag aggaagcccg gctttccgcc cgctataccg
     4741 aactgctcgc ctcggccaag ctggagatcg ccggccagac ggtgaatctc gccgggctgg
     4801 gtccctatgc cgaaaatccc gatcgcgtgg tgcgtcacca ggcagaacag acacgatggc
     4861 ggttcttcga ggcaaacgcc gcgaccctcg acgatctgta cgaccagctt gtcagactgc
     4921 gccacggcat ggcccgcaaa ctgggcttcc agacctacac gccgcttggc tatcggcgcc
     4981 tgcgtcgcgt cgattatggc cccgacgatg tcgcccgtta ccgcgaacag gtcgtccgcc
     5041 atgttgtccc cctggtcggc cgcttgctgg aagcgcgccg cgtggaaaat ggttgggacc
     5101 gcctgcgata ctgggacgag gcgctggtcg atccggccgg caacccgaaa cccgccggcg
     5161 atcatgaccg gctggtggat gccgccgcgg cgatgttcga cggcatggac gacaaattgg
     5221 cctcgttcta tcggatgatg agccagggcg ggttcatgga tctgaaaaac cgcccgggca
     5281 aagccggtgg cgggttctgc acatcgttcc caaccgaggg cgtgccgttc atctttgcca
     5341 acttcaacgg cacccatcac gacatcggcg tttttaccca tgagatggga cacgccttcc
     5401 agaactggga aagccgcaat cagccaaacg tggactacct ttggccgact atggaggcag
     5461 ccgagatcaa ctccatgggt ctggagttcc tgtcctatcc gggcatcggc cgcctggtcg
     5521 gcgaggatgc cgccgaccgc ttccgccgca tgcacctgat cggcgcactg gcgttcttgc
     5581 cctacggcgt ctgcgtcgat catttccagc acgaggttta cgccaagccg gaagcttccc
     5641 cggctgagcg gcatgcgatg tggtcgcgac tggaaaagat ctacatgcca tggaccgact
     5701 acggcgacct ggacttcctg gttaagggcg gccggtggca ggccaagcca catatcttcg
     5761 gctcgccgtt ctactacatc gactataccc tggcacagtg ttgcgcgatg cagttctggg
     5821 tccggtcgcg acgcgattat gccgggacgg tacgagacta cgtcgcattg tgcgcccggg
     5881 gcggatcggc accattccag gatctggtgc ggtcagcggg cctggtatcg ccgttcgccg
     5941 atggcgcgct ggcggatgtc gtcacggaag cggcagcggt gcttaaggtt taggacgcac
     6001 tcgggcgccc cgaagaagcg cggccggatt gacaatcgct ttgcactcgg atggcccgat
     6061 catcgccagc agcggcgacg ctccatagaa acgttcgatc tgtgcaactc ttcaggacaa
     6121 cttgcttaca aaaattcgtg gattccgtag gctgctgctg ggcgttccat cggggttcgt
     6181 gcgatgggta catggcaatc atcgcttccc ggtggcaaat ccgccaaaat caccgaccag
     6241 gcaacg
//
LOCUS       DASWQN010000687         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_198484, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000687 DASWQN010000000
VERSION     DASWQN010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198484"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VGC82_11675"
     CDS             complement(<1..542)
                     /locus_tag="VGC82_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0423971.1"
                     /translation="MIKIEPAPKGDHTRGLGGFAAGFFAAFSRNKRSIAVDLKKPEGQ
                     AVIHRLVPTADVVLENYGPGTMERLGCGYDQLAPLNPRLIYLALKGYLAGPYEHRPAL
                     DEVVQFQTGLAFMTGPIGQPLRAGASVIDILGAVFGVVAAQAALREREQTGKGQRVGS
                     ALFESAAFLMSTHMAGMTATG"
     gene            complement(539..649)
                     /locus_tag="VGC82_11680"
     CDS             complement(539..649)
                     /locus_tag="VGC82_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0423972.1"
                     /translation="MTGTSATPLAGLRVLEFSHTVMGPTAGLIFAELGAM"
     gene            complement(646..2004)
                     /locus_tag="VGC82_11685"
     CDS             complement(646..2004)
                     /locus_tag="VGC82_11685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0423973.1"
                     /translation="MTERRQLRLGAFMRPVSIHTAWWRYPGAYPDANFNFRHIAGFAQ
                     TLERGKFDAFFMADHLALLNMPMNALKRSATTTSFDPLTLLPALAVLTERIGLIATAS
                     TTFNDPYHIARKFASLDHISNGRAAWNLVTTSNPEAALNFGLQEHVEHGERYLKAREF
                     YDVVTGLWDSWADDAFIRDVENGVYFDPERMHVLNHKGAHFSVRGPLNIARPVQGWPV
                     IVQAGASEAGRQIAAETAEMVFAAGGPMADAQRFYADVKGRMETLGRNPNHLKIMPGA
                     LVVVGETVAEAQKKRDLLDSLVHPDSGIGSLSIALGCDASKFDLDGPLPEIPETNQSK
                     SGRQRVIDRAKRDNLTVRQLAQVAGGYGGLSFTGTPETIADQMEEWLFTDACDGFNVM
                     FPYVPGGLDDFVNMVFPELQRRSLFRKEYEGRTLRENLGLPRPENRFFPTNPNRVQEH
                     GR"
BASE COUNT          400 a          708 c          801 g          437 t
ORIGIN      
        1 ccagtcgcgg tcatgccggc catgtgggtg ctcatgagga aggcggcgct ttcgaacaac
       61 gcgctgccga cccgttggcc tttgccggtc tgctcacgct cccgcagggc ggcttgggcg
      121 gcgacgacac caaagaccgc ccccaggatg tcgatcaccg aggcgcccgc ccgcagcggc
      181 tggccgatcg ggccggtcat gaaggcgagg ccagtctgga actggacgac ttcgtccagg
      241 gcgggacggt gttcatacgg tccggccaga tagcctttca gggccaggta gatcagccgg
      301 gggttcagcg gcgcgagttg atcgtagccg caaccgaggc gttccatcgt gcccggccca
      361 tagttttcca gcaccacgtc ggcggtcggg acaagccggt gaataaccgc ctgcccttcc
      421 ggctttttca gatccaccgc gatgctgcgc ttgtttcggc tgaaggcggc gaaaaagccg
      481 gcggcgaagc cacccaggcc cctggtgtgg tcgcctttcg gagcgggctc gatcttgatc
      541 acatggcgcc gagttcggcg aatatcagac cggcggtcgg acccatgacg gtgtggctga
      601 attccaggac ccgaaggccg gcaagaggcg tcgcggacgt tccggtcatc gcccgtgctc
      661 ttggacgcgg ttcgggttgg tcgggaagaa ccggttctcc ggccgtggca ggcccagatt
      721 ttcccgaagc gtccggcctt cgtattcctt gcggaacagg cttcgacgtt gcagttcggg
      781 gaagaccata ttgacgaaat cgtccaggcc gccggggacg taggggaaca tgacgttgaa
      841 gccgtcgcag gcgtcggtga acagccactc ttccatctga tcggcaatgg tttccggtgt
      901 gccggtgaac gacagcccgc cgtagccccc ggccacctgg gcgagttggc gcaccgtcag
      961 gttatcccgt tttgcccggt cgatgacgcg ctgtcggcca cttttgcttt ggttggtttc
     1021 ggggatttcg ggcagcggac catcgaggtc gaacttcgat gcgtcgcagc ccaaggcgat
     1081 cgacagcgac ccgatgccgc tgtcggggtg caccaggctg tcgagcaaat cgcgtttctt
     1141 ttgcgcctcg gctacggttt cgccgaccac gacgagcgcg ccgggcatga ttttcaggtg
     1201 gttggggtta cggcccaggg tctccatgcg ccccttgacg tccgcgtaga agcgctgcgc
     1261 gtcggccatt gggccgccgg cagcgaacac catctccgcg gtttcggcgg cgatctgccg
     1321 gccggcttcc gacgcgccgg cctggacgat gaccggccag ccctgaaccg gtcgggcgat
     1381 attgagcgga ccccggaccg agaaatgggc gcccttgtgg ttcaggacgt gcatgcgttc
     1441 ggggtcgaag tacacgccgt tttccacgtc gcggatgaag gcgtcgtcgg cccaactgtc
     1501 ccacagtccg gtcaccacgt cgtagaactc gcgtgctttc aggtatcgct cgccgtgctc
     1561 gacgtgctcc tgcaggccaa agttcaacgc ggcctcgggg ttggatgtgg tgaccaggtt
     1621 ccacgccgcg cggccgttgc tgatgtggtc cagcgaggca aacttccggg cgatatggta
     1681 cgggtcgttg aacgtggtcg aggccgtggc gatgaggccg atccgttcgg tcaggacggc
     1741 gagcgcgggc aggagcgtca gcggatcgaa cgatgtcgtg gtggcactgc gcttcagggc
     1801 gttcatcggc atgttcaaca acgccagatg atcggccatg aagaaggcgt cgaacttgcc
     1861 gcgctccagc gtctgcgcga agccggcgat atggcggaag ttgaagttgg cgtccgggta
     1921 ggcgccggga tagcgccacc acgcggtgtg gatactgact gggcgcatga aggcccccag
     1981 ccgcaattgt cgccgttcgg tcatggccat ggctccattg ctacatccgg caggctaata
     2041 tgccgaacca ggagttgcca tacttggcga acctcgaatt tgcggcggcg gtaaatcgct
     2101 tcgacgaatt gaccgattga ggggcttctt agtcgcgctg gcaaaatgcc aggagcagcc
     2161 gggccccagg agcagacagc ggatgtcatg cgaaaactgg gcaacctcag ccggtcctga
     2221 gcgcggcttc aaagccggcg gacgcggggc atgatctccg tcgcgaagcg gcgcatttca
     2281 gcctcgaacg gctggaactg gagcataaac aactcgatgc cggcgtcggc gaagtggcgg
     2341 atgcgg
//
LOCUS       DASWQN010000688         3623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_594292, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000688 DASWQN010000000
VERSION     DASWQN010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594292"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1036)
                     /locus_tag="VGC82_11690"
     CDS             complement(62..1036)
                     /locus_tag="VGC82_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0423974.1"
                     /translation="MVGSLTALRPAAAADVTLLNVSYDPTRELYRATDRAFAQAWQAK
                     TGQLVTVNQSHGGSGAQARAVLDGLQADVVTLALAADIEALSKRGLIAPGWQSRLPDN
                     AAPFTSTIVLLVRKGNPKGIHDWPDLLKPGVAVITPNPKTSGGARWNFLAALGWAQHQ
                     PGATAAQAERFVRDLFHQVPVLDTGARGATNSFAQRDLGDVLLAWENEAWLARKEFGA
                     DAYEIVYPSLSILAEPPVAVVDSVVDKRGTREVAQAYLEFLYTARAQDIAARNYYRPR
                     DASVAARYAERYPTLNLVSIQDFGGWDKAQAMYFADGGMFDRIYTPGK"
     gene            complement(1551..2792)
                     /locus_tag="VGC82_11695"
     CDS             complement(1551..2792)
                     /locus_tag="VGC82_11695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0423975.1"
                     /translation="MHMMYFTEQPMAAYDAKAGLDFGATALNFSNKYFDPKEGSRLYN
                     EYLEEYIMVEECGADGIMLNEHHNAPFCMQARANMFAAILAAVTKRVKIVMLGNPLPL
                     TENPVQLAEELAMIDMISKGRLVSGFVRGGGQEQLANGVNPAFNRERFVEAHDLVQKT
                     WTEPGPFRWEGTHYQQRVVNPWAIPLQKPYPRVWIPGVLSKETVIWTAQQRYPYIALN
                     TSIEATKTIHETYDKAAADVGYTAGPENFGYLIRVHVQDTEEKAMQNARQFMWMQGEF
                     TGLANPVWANPAGYFSPSGRRAFVEFSTGRAKNPRGNPTFEQQVADTMIIAGTPDQVI
                     ERLKYLVKETRPGIMGIWANDGTVSKEDRRRCIQLLCGEVMPAIKEYGAGLGLKDPWE
                     ANAPVHLRYSTDIPAQRVAAE"
     gene            complement(2806..>3623)
                     /locus_tag="VGC82_11700"
     CDS             complement(2806..>3623)
                     /locus_tag="VGC82_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0423976.1"
                     /translation="ILSFEVREGNPRMSVVWTDTVKEIAGIKLHLSRAGSGPTVLVLH
                     HDIGTVDRLPFYDQLATKFDVLVPHHPGWGKSERPQWMRHPRDLGAIYSWLQADLDVS
                     GVSLIGLGFGGWIAAEMASQSPRTYKKLVLVGAMGIKPPEGDIADQAIVSYLDYPRSG
                     FHDQAAFASVYGDVTTDQLEAWDIAREMSFRTAWKPYMYNQTLPHLLGGVRTPALIIW
                     GDDDRIVPLSAGQAYARALSGATLTTIPACGHFAEMEKPDAVANLAVEFLSRS"
BASE COUNT          635 a         1151 c         1091 g          746 t
ORIGIN      
        1 ggcattttcc cgcccggcat ttccccaccc ggcgtttccc cgacggtcat tttcccgacg
       61 gtcattttcc cggcgtgtag atacggtcga acatcccgcc atcggcaaaa tacatcgcct
      121 gggctttgtc ccagccaccg aaatcctgga tcgagacgag gttcagcgtc ggatagcgtt
      181 cggcgtaccg cgccgcgacg gaagcatcgc gcggccggta gtagttgcgt gccgcaatgt
      241 cctgtgcccg ggccgtataa agaaattcca gataggcctg cgccacctca cgcgtgcccc
      301 gcttgtccac gacgctatcc accaccgcca ccggtggttc ggccaggatg ctcagcgagg
      361 gatatacgat ctcataggca tcggcaccga attctttccg agcgagccac gcttcatttt
      421 cccaggccag cagcacgtcg cccagatcgc gttgcgcgaa actattggtg gctccgcgcg
      481 caccggtgtc caggaccggc acctggtgaa acaggtctcg tacgaaacgt tcggcctggg
      541 cagcggttgc acccggctgg tgctgcgccc aacccagcgc ggcgagaaaa ttccatcgcg
      601 cgccgccgga ggttttgggg ttcggcgtga tgacggccac gcccggcttc agcaagtccg
      661 gccaatcgtg gatgcctttc ggattgccct tacgcaccag cagcacgatc gtgctggtaa
      721 acggcgccgc attgtccggc aggcggcttt gccagccggg agcgatcagg ccacgcttcg
      781 acaatgcctc gatgtcggcg gccagtgcca gggtcacgac atccgcttgc agaccgtcca
      841 gcacggcccg cgcctgcgcg ccggatccgc catgggactg gttgacggtg acaagctgcc
      901 ccgtcttggc ttgccaagcc tgggcgaatg ctcggtcggt cgcacggtat aactcccgcg
      961 ttggatcgta ggagacattc agcagggtaa cgtccgccgc cgcggcaggc cgaagcgccg
     1021 tgaggcttcc gaccatcgca gcaagcgcgg ttctgcgggt caatcgatca gacatgatgc
     1081 atctccggta ggcatgcctt tcactgagaa ggattttgtt gttggtcaac aacggtctgg
     1141 ttcgctgcca ggccgaacat gtggaattct actgtcgcca acgttttgct gtcgccaacg
     1201 tgctgctgtc gctaacgtgc tgggcggggc acaacattgc gacacagata cagaacgaca
     1261 ggccttgctt ctcgttatct ctgattattc cggatacaag ctcgttctgt ccatcggcag
     1321 cccaatggct tagcgcattc tgtaggttaa cgcgcgacaa ggccagcatt ttcagccggt
     1381 cggcagcctt acgatatgac cccgcccgat ggcgcttcgg atgccgcgcc ttggcgtcgg
     1441 tgcatatagg gcgtctggcg gctttgttta tccgcaggag gccgtggatg ccggcatcgc
     1501 gcaatgcagc gccggggtgg cgggaaatcc agccaccccg gctatttcag ctactccgcc
     1561 gcgacgcgct gcgccgggat gtcggtcgaa taacgcagat gcaccggcgc attcgcctcc
     1621 caagggtcct tcagtcccag tccagcgcca tactccttga tcgccggcat cacctcgccg
     1681 cacagcaact ggatgcagcg gcggcggtct tccttgctga ccgtgccgtc gttggcccag
     1741 atccccatga tgcccggacg ggtttccttc accaggtatt tcagccgctc gatcacctgg
     1801 tcgggcgtcc cggcaatgat catggtatcc gcgacctgtt gctcgaacgt cgggttgccg
     1861 cgtgggttct tggcgcgccc ggtggagaat tccacgaacg cccggcgtcc ggatggcgag
     1921 aaatatcccg cgggattggc ccagacggga ttggccagtc cggtgaattc accctgcatc
     1981 cacatgaact ggcgggcgtt ctgcatcgcc ttttcttcgg tgtcctgcac atgcacccga
     2041 atcaggtaac cgaaattctc cgggcccgcg gtatagccca cgtcggcggc ggctttgtca
     2101 taggtctcgt ggatggtctt ggtcgcttcg atcgaggtgt tcaacgcgat gtacggatag
     2161 cgctgctgcg ccgtccagat cacggtttcc ttcgacaaca cgcctggtat ccacacccga
     2221 ggatacggct tttgcagcgg gatcgcccag gggttcacca cgcgctgctg ataatgcgtg
     2281 ccttcccagc ggaatggtcc tggctccgtc caggtctttt gcaccaaatc atgtgcctcg
     2341 acgaaccgct cgcggttgaa cgccgggttc acgccgttcg ccaactgctc ctgtccgccg
     2401 ccgcgcacga agccggacac cagccggcct ttcgagatca tgtcgatcat cgccagctct
     2461 tccgcgagct gcaccggatt ttcggtcagc ggcagcgggt tgcccagcat gacgatcttc
     2521 acgcgcttgg tcaccgcggc caggatggcg gcgaacatgt tggcccgcgc ctgcatgcag
     2581 aacggagcat tgtggtgctc gttcagcata atgccgtcgg cgccgcattc ctcgaccatg
     2641 atgtattctt cgaggtactc gttgtagagc cggctcccct ctttcgggtc gaagtacttg
     2701 ttggagaagt tcagcgcggt cgcgccaaaa tccagcccgg ccttcgcgtc gtaggccgcc
     2761 atcggctgtt cggtgaaata catcatatgc atggtgaggc ctccttcagc tacgagacag
     2821 aaattcgacc gcgagattcg cgaccgcgtc gggtttttcc atttcggcga aatgtccgca
     2881 ggccggaatg gtcgtcaagg tcgcgccgga cagtgcgcgc gcataggcct ggcctgcgct
     2941 caatgggacg atccggtcgt cgtcgcccca gatgatcagg gccggggttc gcacgccgcc
     3001 caacaggtgt ggcagagtct ggttgtacat gtagggtttc cacgcggtgc gaaagctcat
     3061 ttcgcgggcg atatcccagg cttccaactg atcggtggtg acgtcgccat agaccgatgc
     3121 gaaggctgcc tggtcgtgga agccggaccg cgggtagtcg aggtacgata cgatcgcctg
     3181 gtcggcgata tcgccttcgg gcggcttgat tcccatggcg ccgaccagca ccagcttctt
     3241 gtatgtccgc ggcgactggc tggccatctc ggctgcgatc cagccgccga aaccaagacc
     3301 gatcagggat acgccggata cgtcgagatc cgcctgcagc caactgtaga tcgcgcccag
     3361 atcccgtgga tggcgcatcc attgcggccg ctccgacttg ccccagccgg gatggtgcgg
     3421 gaccaacaca tcgaatttcg tggccaactg gtcataaaaa ggcaggcggt ccacggttcc
     3481 gatatcgtga tgcaggacca gcaccgtcgg cccactcccg gctcgggaca gatgcagctt
     3541 gatgccggcg atctctttga ccgtatcggt ccagacgacg ctcattcttg ggttcccttc
     3601 tcggacttcg aatgacagga tac
//
LOCUS       DASWQN010000689         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2441507, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000689 DASWQN010000000
VERSION     DASWQN010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2441507"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="VGC82_11705"
     CDS             complement(<1..1287)
                     /locus_tag="VGC82_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEY0423977.1"
                     /translation="MIFVAPWVLGGLLTLPLLWWLLRVTPPAPRSEIFPAVRFLLGLN
                     ATEETPARTPWWLLALRLVAAGLVIVALARPVLDAGTALVGNGPVLLVMDNGWAAADD
                     WVRRKEAAGALLDRAERAGRKAALLATAPNGSGAPVAISPTMPVADLRARLAAMQPLP
                     WPPDRAAAADAMRDWSDRTDASVIYVPDGLTDGADFGRFASAMRDVGPVTEVCCDAAS
                     SPLLLPPASEADRLVIHLAQAPRATATEASVLAQSGDGRTLARTSITVPAGASAASGP
                     ISLPPELRNRLTRLVLDGPPSAGSVVLLDERWRRRPVGLLPGDLTTADTPFTGSIYFL
                     KRALAPFSELREGDAATLLKRDMSVLVLADRPLPDGPERDALEAWVRKGGLLIRFAGP
                     RTAEAATNDTDTLLPVRLLGGDRQLGGAMSWSQPAGL"
     gene            complement(1284..2156)
                     /locus_tag="VGC82_11710"
     CDS             complement(1284..2156)
                     /locus_tag="VGC82_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0423978.1"
                     /translation="MANDALTAEALGARLPPLLIAADRVASTVAQGVHGRRRVGQGDS
                     FWQFRRFVSGDPLSRIDWRQSAKSGRGAPDGWFIRETEWEAAQTVCLWRDASASMRWR
                     SRQVPVEKRDRAGLLLLSLASLLLRGGERVMMMQQGARSVTSRSGLERLVAELEAVDD
                     DAGLPPDVRLPRHGTVVLFSDFLSPLDEIRALIGRFAGVPVTGYLLQILDPAETALPY
                     QGRVRFRGLESDGDALIPRVESVREAYAERLKAQQDGLAAICATAGFGFGIHRTDHPP
                     EMALLTLYQALAAR"
BASE COUNT          498 a          938 c          823 g          389 t
ORIGIN      
        1 gaggccggcc ggctggctcc aggacattgc cccgcccaac tggcggtcgc ctccaagcag
       61 ccgcacggga agcaaagtgt cagtatcgtt ggtcgccgcc tccgcggttc gggggccggc
      121 aaatcggatc agcaatccac ccttgcgcac ccatgcctcc aacgcgtcgc gttccggccc
      181 gtccggcaag ggccggtcgg ccaacacaag gacggacatg tcacgtttca ggagtgtcgc
      241 cgcatcgcct tcccgcagtt cggagaacgg cgccagcgct cgcttcagga agtagatcga
      301 cccggtgaac ggcgtatcgg ccgtggtgag atcgcccggc agcaggccca ccggccgccg
      361 ccgccatcgt tcgtccagca gcacgaccga cccggcgctc ggtggcccgt ccaacaccag
      421 ccgcgtcagc cggttacgta gttccggcgg cagggaaatg ggtccggatg cggccgacgc
      481 acccgccggt acggtgatgc tggtccgagc caaggtacgg ccatcgccgc tttgtgccag
      541 cacgctggct tcggtagcgg tcgcgcgagg ggcttgcgcc agatgaatga ccagtcgatc
      601 cgcctcactg gcgggcggca aaagcagcgg cgaggacgcc gcatcgcagc aaacttccgt
      661 caccggacca acatcgcgca tggcggaggc gaagcgcccg aaatccgcgc catcggtcag
      721 gccgtccggc acataaatta ccgatgcatc cgtccggtcc gaccaatccc gcatcgcgtc
      781 cgctgcggcg gcgcgatccg gtggccatgg caagggctgc atcgcggcca agcgggcacg
      841 cagatcggcg actggcattg tgggagagat tgcgaccggg gcgccgctgc cattcggtgc
      901 ggtggccaac aatgcggctt tgcgtccggc acgttcggca cggtccaaca acgcgccggc
      961 cgcttccttc cggcgtaccc agtcgtccgc cgccgcccag ccgttatcca tcaccagcag
     1021 caccgggccg ttgcccacaa gcgcggttcc ggcatccagc acagggcgcg ccagtgcgac
     1081 gatcaccaaa cccgcagcca ccagccgaag ggccagcagc caccatggcg tccgcgccgg
     1141 ggtttcctcg gttgcgttca acccgagcag gaaacggacc gccggaaaga tctccgaccg
     1201 cggtgccggc ggggtaaccc ggagcagcca ccacagcagc ggcaaggtca acagaccccc
     1261 gagcacccat ggggcgacga aaatcatcgc gccgccaggg cttgatagag tgtcaacagc
     1321 gccatctcgg gcggatgatc ggtgcggtgt atgccaaagc cgaacccggc ggtggcgcaa
     1381 atcgcggcca acccgtcttg ctgcgccttc aagcgttcgg cgtaggcttc gcgcacggac
     1441 tcaacccgcg ggatcagcgc gtcgccatcg ctttccaggc cgcgaaaccg cacacggccc
     1501 tggtagggca gggcggtttc cgccggatcg aggatttgca gcaaatatcc cgtcaccggc
     1561 accccggcaa atcggccgat caacgcgcgt atttcatcga gcggcgacag gaagtcgctg
     1621 aaaagcacca ccgtcccatg gcgtggcaac cgcacgtccg ggggcagacc agcatcgtca
     1681 tcgacggctt ccagctcagc caccagtcgc tccaggccgg atcggctggt caccgaacga
     1741 gcaccttgct gcatcatcat caccctttcg ccgccgcgaa gcagcaggga ggccaatgaa
     1801 agcaacagca gaccggctcg atcgcgtttt tcgaccggca cctggcgcga acgccagcgc
     1861 atggaggccg aagcatcccg ccacagacag acggtctggg ccgcttccca ttcggtctcg
     1921 cggatgaacc agccgtcggg cgctccgcgg cctgacttcg cggattgccg ccaatcgatc
     1981 cgcgacaacg gatcgcccga gacgaagcgc ctgaactgcc agaaactgtc accctgcccg
     2041 acacgccggc ggccatgcac gccctgggcc accgtagagg ctacccgatc cgccgcgatc
     2101 aacaacggcg ggagccgggc acctaacgcc tcagcggtca gggcatcgtt tgccatgcat
     2161 catcctttgt ctgccatgca tcatccttcg ttgggccggc gtgaaacgtc cccgggatgt
     2221 tcgctgctgg ataccgccgc gcggcagagg ccacacgggc gtcaataagg cgccatcgac
     2281 gcccaggatc tggaccgcgc accatcgccg ggctcgatgc cattatggaa cagggtgctt
     2341 ttatagtgtc atcgccgttc ttggcgcggc tacctgcgac cgcacctaca tgccgggaag
     2401 tcgtggattg ccgggacagg gccggccata accgatttgg caccgaccag gcggtggccg
     2461 gcaactccgg gcacatgaag ccaccgtccg tcacgagatg cccgccgaca ccttgcccga
     2521 cgccgacacc ctgcccgatc atcacaaggc cgtgttgccg cagggcgtgg tcgggcatgc
     2581 ggctcaggaa aaacctaccc aatactgcca accaatcggt cgatcacatt ctccagcgcg
     2641 attccgtc
//
LOCUS       DASWQN010000690         4317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2574246, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000690 DASWQN010000000
VERSION     DASWQN010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2574246"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VGC82_11715"
     CDS             complement(<1..287)
                     /locus_tag="VGC82_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0423979.1"
                     /translation="MTCLFPHLSRLRLVACLALLAGLGACGTHPSHRTTAHYSPSRYY
                     PPPGPASDPWGPYIHEASARFTVPENWIRRVMRQESGGQEDVISWAGAMGL"
     gene            complement(477..1241)
                     /locus_tag="VGC82_11720"
     CDS             complement(477..1241)
                     /locus_tag="VGC82_11720"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0423980.1"
                     /translation="MNLFDLKGKVAVITGGNGGIGLGMARGIASLGASIVIAGRDEGK
                     AATALTTLREMNAEASFVAADVTKKAACQALIAAATEKYGRIDILVNNAGTSVRKMPQ
                     DLSEDEWHHVLDTNLTSAFFCSQAAYPEMVKAGGGKMINIGSMMSLFGAPYAVAYASS
                     KGGIVQMTRAMATAWAKDNIQVNAVLPGWIDTDLTKRARQQVTGLHDSVEKRTPHGRW
                     GTSDDMAGIAAFLASSASDFVTGTAIPVDGGFSIAM"
     gene            complement(1467..3461)
                     /locus_tag="VGC82_11725"
     CDS             complement(1467..3461)
                     /locus_tag="VGC82_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY0423981.1"
                     /translation="MGHGSERAFGNEGGACMSGTRNGRSPRSVALVGPYSSGKSTLFE
                     ALLEAAGAPVKRPADPRNRPMTTEIRLGHCNYLGDSWSILDCPGSIEFAYQACSALAM
                     VDIAVVVCEPSPAKAAAVAPLLKTLKDEGIPHIVFINKIDTLEGSLADTLASLQAFAG
                     CPLVLRQMPIFDGESVSGYVDLMSERAYRYRRGEASELMQIPASMLDEEQAALGRLVE
                     TLADRDDALLEKVLEDIKPTPEELYRDMRKDLLDGAVIEVLVGAAEHANGVRRLWKAL
                     RHDTPEPAETANRKGIDPNGPALAQIFRTAYAGHTGKLSYARVWSGTIADGAHLGGTR
                     LGGIYHYANGELTKVPEAKQGDIVAFGRLDGVPTGATVSPGAQPEPLPFPRPTPPVYA
                     MAIATTDRKDDVKLSGALHKLAEEDASLFITQDGETVLHGQGEMHLQTTVEHLAKDYN
                     LRLATHKPAIAYKETIRKPVHEHGRLKRQTGGHGQFADVKIDIAPRGRGEGFQFVDKI
                     VGGAVPRNFIPAVGEAAEEATRKGPFGFPVVDVSVTLVDGGFHSVDSSDMAFKTATQI
                     AMKEALAKADPVLLEPVDHVTISVPNDYTPRAQRLLTGRRGQILGFAERQDWPGWDDV
                     EALVPEAELHDLIIELRSQTMGLGTYRKRFDHLAETRTVP"
     gene            complement(3627..4190)
                     /locus_tag="VGC82_11730"
     CDS             complement(3627..4190)
                     /locus_tag="VGC82_11730"
                     /inference="COORDINATES: protein motif:HMM:NF027286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEY0423982.1"
                     /translation="MPTFRMRRRVWVYLAAGTVAMTFLGRLVTETSAAPDQIYRLAPF
                     IGSLLVAVGWIVTSEVNIGNSRRQHTITLITQHAFDAQRAANRDIIKQSLPSFRTRLQ
                     PGPTIDFDDENATLLKAIDLELNFYEFLAVGVASGNLDERLIRESLGSQFKAFYIQVE
                     AYIAHWRAKNDKTWRELSRMYTRWLGI"
BASE COUNT          728 a         1349 c         1403 g          837 t
ORIGIN      
        1 atcagcccca tcgctccggc ccaggagatc acgtcctcct gcccgccgga ttcctgacgc
       61 atgacacggc gaatccagtt ttccggcact gtgaagcgtg cggaggcttc gtggatgtag
      121 ggcccccagg ggtcgctggc cggtcccggc ggcgggtaat agcgggatgg actgtagtgg
      181 gcggtcgtgc ggtgggaagg atgagtgccg cacgcgccga ggccggccag caaagccagg
      241 caggcgacca gccggagccg ggaaaggtgc gggaacaggc aggtcatgga caggtcaccg
      301 gcaaggtggt tccgccgttg ttaccacaga agctgaagga cgtggtagaa atctatgcca
      361 gcgttacgaa cttggcccgg gaagcctacc gggtagcaat gatatttggc aatcaagttc
      421 ggcatggctg gttgggcaag cgccagccgg acgccaaggg cggcaacctg gcgcgatcac
      481 atcgcaatcg aaaatccgcc atccaccgga atcgccgtgc cagtcacaaa gtcggatgcc
      541 gatgacgcaa gaaacgcggc aattccggcc atgtcgtccg aagtgcccca tcggccatgc
      601 ggcgtgcgtt tctctacgct gtcgtggagt ccggtcacct gctggcgcgc gcgcttggtc
      661 aggtcggtgt cgatccaacc gggcaacacg gcgttcacct ggatattgtc ctttgcccac
      721 gcggtggcca tcgcgcgcgt catctgcacg atcccgccct tgctggacgc ataggcgacc
      781 gcataaggcg cgccgaacag cgacatcatc gagccgatgt tgatcatttt gccgccgccc
      841 gccttcacca tctcggggta ggcggcctgc gagcagaaga aggcactcgt caggttggta
      901 tccaggacgt gatgccattc gtcctcggac aggtcctgcg gcatcttgcg gactgacgtg
      961 ccggcattgt tcaccaggat gtcgatgcgg ccgtatttct cggtggcggc ggcgatcagc
     1021 gcctggcacg cagccttctt ggtcacgtcg gcagcgacga agctggcttc tgcattcatc
     1081 tcccgcaggg tggtcagggc ggtggcggct ttgccttcgt cacgtccggc aatgacgatg
     1141 ctggcgccga gcgaggcgat gccgcgtgcc atacccagcc cgatcccacc gttgccgccg
     1201 gtgatgaccg ccacttttcc ctttagatcg aacaggttca tcggacattt tcccgcttga
     1261 ggcatgagcg cgccgggcgg atggccaaag cgcgtattca tgttgacggg aaacaagtcg
     1321 gtttgtcact gccatcgaca tgggcgccgc tgggaccgcc aaacaaaacc cttggtcggg
     1381 acgtgccgcg gcatgagggg cgacctcatg ccgcgcaaga cgtgacctca tgctgcgcaa
     1441 gacgtgacct catgctgcgc aagacgtcag gggaccgtgc gggtttccgc cagatggtcg
     1501 aaccgcttgc ggtatgtgcc caggcccatg gtctgcgacc gcagctcgat gatcagatca
     1561 tgcagttccg cctcgggaac caacgcttcc acatcgtccc atcccggcca gtcctggcgt
     1621 tcggcgaagc cgaggatctg cccgcgccgg ccggtcagca ggcgctgcgc ccggggcgtg
     1681 tagtcgttcg gcacgctgat ggtaacgtgg tccaccggtt ccagcagcac cggatcggcc
     1741 ttggcgagcg cctctttcat cgcgatctgg gtagcggtct tgaacgccat gtcggagctg
     1801 tccaccgagt ggaagccgcc gtccaccagg gtgaccgaca catccaccac gggaaagccg
     1861 aacgggcctt tgcgggtggc ttcctcggcg gcctcgccca cagccgggat aaagttgcgt
     1921 ggcaccgcgc cgccgacgat cttatcgacg aactggaagc cttcgccgcg cccccttggg
     1981 gcgatgtcga tcttcacgtc ggcgaactgg ccgtgtccgc cggtctggcg tttcaggcgg
     2041 ccgtgctcat gcaccggctt gcgaatcgtt tctttgtagg cgatcgccgg cttatgggtt
     2101 gccagccgca ggttgtagtc cttggccagg tgctccaccg tggtttgcag atgcatctcg
     2161 ccctggccgt gcagaacggt ttcgccgtcc tgggtgatga acagggatgc gtcttcctcc
     2221 gccagcttgt gcagcgcgcc ggacagtttc acgtcgtcct tccggtcggt ggtggcaatc
     2281 gccatcgcgt acaccggtgg cgtcgggcgc gggaacggca gcggttcggg ttgcgcgccg
     2341 ggcgaaacgg tggcgccggt gggtacgcca tccaggcgtc cgaacgctac gatgtcacct
     2401 tgtttcgctt ctgggacctt tgtcagttcg ccattcgcat agtgatagat gccgcccagg
     2461 cgcgtgccgc caaggtgcgc gccgtcggcg atcgtgccgc tccagacgcg ggcataggac
     2521 agtttgccgg tgtgcccggc gtaggcggtg cggaagatct gggcgagcgc cggcccattc
     2581 gggtcgatcc ccttgcggtt ggcggtttcc gccggctccg gcgtgtcgtg ccgcagcgct
     2641 ttccacagcc ggcggacgcc attggcgtgt tcggcggcgc cgaccagaac ctcgatgacc
     2701 gcgccgtcca gcaggtcctt gcgcatgtcg cggtagagtt cttcaggcgt cggctttatg
     2761 tcctccagca ctttttccag cagcgcgtcg tcacggtcgg ccagcgtttc caccagccgg
     2821 cccagcgcgg cctgctcctc gtcgagcatc gaagccggga tttgcatcag ttccgaggcc
     2881 tcaccgcggc gatagcggta ggcgcgctcg ctcatgaggt cgacgtaacc ggaaacgctc
     2941 tcgccgtcga agatcggcat ctgccgcagc accagcgggc aaccggcaaa ggcctgcaga
     3001 ctggcgaggg tatcggcgag gctgccttcg agcgtgtcga tcttgttgat gaagacgata
     3061 tgcgggatgc cttcgtcttt tagcgtcttg agcagcggcg ccacggcggc ggccttggcc
     3121 ggcgacggct cgcagacgac gaccgcgata tcgaccatcg cgagggcgga gcatgcctgg
     3181 taggcgaatt cgatcgagcc gggacagtcc aggatcgacc aggaatcacc caggtagttg
     3241 cagtgtccca gtcggatctc ggtggtcatc gggcggttgc gtggatcggc cggtcgcttg
     3301 acaggggcgc ccgctgcttc cagcagcgct tcgaaaagcg tggattttcc gctgctgtaa
     3361 ggccccacca gagccaccga gcgaggactt ctgccgtttc ttgttcctga catgcacgca
     3421 cctccttcgt ttccaaaggc gcgttccgag ccgtggccca tgcgaagcgg gcactcggtg
     3481 cgccttcgcc cggagatgct agcgcaaaac cattgcaagg ggagtcccct ttcatgaccg
     3541 cgccgccagc cgcccgttgc agggcatcac cgctgttctt tcccaggtcg gtacaatccg
     3601 gaaattgcgc cgatccggcc ggcgaatcag atgcccagcc agcgtgtgta catccgcgac
     3661 agctcgcgcc atgtcttgtc gttcttggcg cgccaatggg cgatataggc ttcgacctgg
     3721 atgtagaacg ccttgaactg gctccccagg ctttccctga tcaggcgttc atccaagttc
     3781 ccgctggcaa cgccgacggc gaggaattcg tagaaattca actccaggtc gatcgccttc
     3841 agcagagtcg cgttttcgtc gtcgaaatcg atcgtcgggc ctggctggag ccgggtccgg
     3901 aaactgggca gggattgctt gatgatgtcg cggttggcgg cccgctgcgc gtcgaacgca
     3961 tgctgcgtga tcaacgtgat ggtatgctgg cgcctggagt taccgatgtt aacttccgag
     4021 gtgacgatcc atccgaccgc gaccagaagg gaccctatga acggcgcgag gcggtatatc
     4081 tggtcaggag cagccgacgt ctcggttacc agccggccga ggaacgtcat cgcgaccgtg
     4141 ccggcggcca gatagaccca gactcggcgg cgcatgcgaa aggtcggcaa gtttcatccc
     4201 cggcttgtca tagccggaga accgacccag gcgtctgtgg ttcaggagcc cggcttggta
     4261 tcaagcatcg acatgtcttt gaccctgtgg ctacttcgat cttaccatat agcttcg
//
LOCUS       DASWQN010000691         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_594586, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000691 DASWQN010000000
VERSION     DASWQN010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594586"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..>1508
                     /locus_tag="VGC82_11735"
     CDS             172..>1508
                     /locus_tag="VGC82_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0423983.1"
                     /translation="MRRRTFLATATVTASTALPRFAIAQSARARTLRFVPQANLTLLD
                     PIFTTALVSVNHGWAIYDMLFSVNRQYEIRPQMAEGYSLSDNGRTYLIKLREGLKFHN
                     GEPVRAQDCAPSLQRWAMRETIGQSLNKAVDVWGVQDDRTLKITLKYPLPIFLDAIAR
                     GAASVPFIMPEHVAKTDPYKQITDYTGSGPFRFIKDQFVPGSSVLYLRHEGYVPRSEP
                     ADWTSGGKVAHFDRLEWHVIPDPATATAALRSGEVDWCDQVQPDLVPVLRKHPDVAIG
                     SSNPLGFNGVLRFNHLHPPFDNVGVRRAVMMAVNQTDYLTAITGNDASASRICKSFMP
                     CGMQYGKELGTAAMPGDLDRARAALKAAGYAGEKVVIINPTDFATIAPMGDVTYDLLK
                     QLGMNVEIVATDWGTVTQRRASKEPIAKGGWSILHTWAPSNVISNVVEHPFLRG"
BASE COUNT          299 a          473 c          464 g          272 t
ORIGIN      
        1 atcagggatc tgacgtgccg tcctccacgg tcatggtccg cccgcgccag cgcaaatgca
       61 ggcggcggtc catggataag cagttgcagc aagcataaaa tccgaggttt gcaactgccc
      121 gctttattgc gcatgctcac cgcaaaacaa agaacaggga ggctacgtat tatgcgacgc
      181 cgcacattcc tcgccaccgc caccgttacg gcgtccactg ccctgccccg cttcgccatt
      241 gctcagtccg ccagggcccg caccttgcgc ttcgtgccgc aggccaacct tacactgctg
      301 gacccgattt tcacgacggc attggtgtcg gtcaaccatg gctgggcgat ctacgacatg
      361 ctgttcagcg tcaaccggca atacgagatc cggccccaga tggccgaggg ctacagccta
      421 tccgacaatg ggcggaccta cctcatcaag ctgcgggagg gactgaaatt ccacaacggc
      481 gagccggtgc gcgcgcagga ttgtgcgccc agcctgcagc gctgggcgat gcgcgagacg
      541 atcgggcaat cgctcaacaa ggccgtggac gtctgggggg tgcaggatga ccggacattg
      601 aagatcacgc tgaaatatcc gttgccgatc ttcctggatg cgatcgcgcg cggtgccgcc
      661 tcggtgccgt tcatcatgcc ggagcatgtg gcgaagaccg atccctataa gcaaatcacc
      721 gactataccg gctccggtcc tttcaggttc atcaaggacc agttcgtgcc tggctcctcg
      781 gtgctgtact tgaggcacga aggttatgtg ccgcgatccg aaccggcaga ctggacgtca
      841 ggcggaaaag tagcgcattt cgaccgcctg gagtggcatg tcattccgga tccggccacc
      901 gccaccgcgg cactgcgatc gggtgaggtg gattggtgcg accaggtgca gcccgatctc
      961 gtgccggtgc tacgcaaaca tccggacgtc gccatcggca gttccaatcc gctgggtttc
     1021 aacggcgtgc tgcgcttcaa tcatctgcat ccgccgttcg acaacgtcgg cgtccggcgg
     1081 gcggtgatga tggcggtgaa ccagaccgac tacctgaccg cgatcaccgg aaacgatgcc
     1141 tcggcgtcgc gcatctgcaa gtccttcatg ccgtgcggca tgcaatacgg caaggagctt
     1201 ggcaccgccg ccatgccggg cgatctggat agagcccgag ccgcgctgaa ggctgccggc
     1261 tatgcaggcg agaaggtggt gatcatcaat cccacggatt tcgccacgat cgccccgatg
     1321 ggcgacgtga cctacgatct gctgaaacag cttggcatga acgtggagat cgtggcgacc
     1381 gactggggca cggtgactca gcgccgcgcc agcaaggagc cgatcgccaa gggaggatgg
     1441 tcgatcctgc acacctgggc gccgtccaac gtgatcagca acgtggtgga gcacccattc
     1501 cttcgggg
//
LOCUS       DASWQN010000692         4841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2046161, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000692 DASWQN010000000
VERSION     DASWQN010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046161"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1125
                     /gene="rpsB"
                     /locus_tag="VGC82_11740"
     CDS             343..1125
                     /gene="rpsB"
                     /locus_tag="VGC82_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY0423984.1"
                     /translation="MAMPDFSMRQLLEAGVHFGHHTRRWNPRMAPFLFGVRNQVHIID
                     LQQTVPMLDRALRAVRDVTAAGGRVLFVGTKRAAAEHVAEAAKRCGQYYINHRWLGGT
                     LTNWKTITGSIKRLRQIEDLVGGDTQGLTKKEVLDITRDKDKLDRALGGIKDMGGLPD
                     ILFIIDTNKEKLAVEEANKLHIPVVAILDSNSDPTGVTFPIPGNDDAIRAITMYCDLI
                     AGAVLDGISAEMAHSGADIGAREELPPEVLADIEPEAAPATA"
     gene            1150..2061
                     /gene="tsf"
                     /locus_tag="VGC82_11745"
     CDS             1150..2061
                     /gene="tsf"
                     /locus_tag="VGC82_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003623141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY0423985.1"
                     /translation="MAAITAALVKDLREKTGAGMMDCKKALTETDGDVEVAIDWLRKK
                     GLAAAAKKSGRVAAEGLIGVASGTNRAAIVEVNAETDFVARNDTFQEFVAKIAEIALE
                     IGDDLDAINAAPFAPGGHSVAEQLTHMVATIGENMTIRRAKVLSVPQGVVASYMHQAL
                     KPGLGKIGVLAAVEGPGELAALELLGRQVGMHVAASRPEALDIDAVDPAALERERAVL
                     SEQARASGKPENIIEKMVDGRIRKYYEEVVLLEQVWVHDGESRVRAVAKKAGVNLTGF
                     VRFQLGEGIEKPQGPDFAAEVAATAGV"
     gene            2163..2885
                     /gene="pyrH"
                     /locus_tag="VGC82_11750"
     CDS             2163..2885
                     /gene="pyrH"
                     /locus_tag="VGC82_11750"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY0423986.1"
                     /translation="MRQPAVYQRVLLKLSGEALMGRREYGLDTDMVNAIASDIKAVAA
                     MGVQVSVVIGGGNIFRGVSGAASGMDRAQADYMGMLATVMNALAMQTALEKRDCPTRV
                     QSAIPMATVCEPYIRRRAERHMEKGRVVIFAAGTGNPFFTTDTAAALRAAEMKCDALL
                     KGTQVDGVYSADPRKFTDATRYEQLTYHDVLAKDLGVMDAAAISLARENHLPIVVFNI
                     HAPGAFAQVMRGEGAFTRIIEA"
     gene            3058..3627
                     /gene="frr"
                     /locus_tag="VGC82_11755"
     CDS             3058..3627
                     /gene="frr"
                     /locus_tag="VGC82_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY0423987.1"
                     /translation="MATADLNSLKQDLTRRMDGSVETLRREFAGLRTGRAHPGLLDPV
                     KVVAYGTEMPLNQLATVGAPEPRMLTVQVWDKSMSGAVEKAIRDSGLGLNPAADGMMI
                     RVPIPQLTTERRNELAKLAHKYAEGAKISVRGVRRDGMEQIKGYEKKHEIGEDLAKNW
                     AEEVQKLTDQFIKKVDEVLVEKEKDIKQV"
     gene            3723..4481
                     /gene="uppS"
                     /locus_tag="VGC82_11760"
     CDS             3723..4481
                     /gene="uppS"
                     /locus_tag="VGC82_11760"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY0423988.1"
                     /translation="MSSAVTSAVDGGSVIVAGQIVPAHVAIIMDGNGRWAKSRSLPRI
                     AGHREGSRAVRRAVEAAIRSGVSWLTIYAFSSENWRRPAGEIQDLTGLLRRYLRSEIA
                     ELKESGVRLRFIGDRTRFDADIQKDLAAAERDTSDNARLNLTIALSYGARAEIAAAAR
                     AAVAAIRTGCMDEAELDEDVFARFLATAGMPDPDLIIRTSGEQRLSNFLLWQAAYAEL
                     VFLDVLWPDFDHEQFNAALTEFARRERRYGARKD"
     gene            4651..>4841
                     /locus_tag="VGC82_11765"
     CDS             4651..>4841
                     /locus_tag="VGC82_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423989.1"
                     /translation="MAFAALVALISAGLSLEWLGFCHQSKKAATIVLFAALPLSVMLT
                     ASGATVPALGLLAVAMVAG"
BASE COUNT          956 a         1496 c         1561 g          828 t
ORIGIN      
        1 aatctggttg agttcactcg attcatagcg gtccaacaca cttcggaact tgatcacacg
       61 cacaccttgg agggcttccg tccggcgcat ccaggctctc caaagaaccc agtatcaccg
      121 gacagatcgt gtgctaccta acccggacat atcgtgtgct accgacaacg aggcttcgca
      181 accttgcaac cgtcggcatt ctgcgccata tccccgtccg ctggctgatc gccggtaaat
      241 tcacacgcgg ggagcctttc ccagccaggc atggccaggg tccggtgctg acaagcaacc
      301 cccgcggagg caacaaccgg acaacaggaa aggagcacgc tcatggcgat gcccgacttc
      361 tcaatgcgcc agctactcga ggccggcgtt cactttggcc accacacccg ccgctggaat
      421 ccgcgcatgg cgccgttcct gttcggcgtc cgcaaccagg ttcacatcat cgacctgcag
      481 cagaccgtgc cgatgctgga ccgtgcgctc cgcgccgtcc gcgacgtgac cgccgccggg
      541 ggccgggttc tgtttgttgg caccaagcgg gccgccgccg aacacgtggc cgaggccgcc
      601 aagcgctgcg gccagtacta catcaaccac cgctggctcg gtggcacgct gacgaactgg
      661 aaaaccatca ccggttcgat caagcggctg cgccagatcg aggatctggt cggcggcgac
      721 acccagggcc tgaccaagaa agaggtgctg gacatcaccc gcgacaagga caagctggac
      781 cgagcgctgg gcggcatcaa ggacatgggg ggcctgccgg acatcctgtt cattatcgac
      841 accaacaagg aaaagctggc ggtcgaggaa gccaacaagc tgcatattcc ggttgtcgcg
      901 atcctggaca gcaactccga ccccacgggc gttaccttcc cgatccccgg caacgacgat
      961 gcgatccgtg ccatcaccat gtattgcgac ctgatcgccg gtgccgtgct ggatggcatc
     1021 agcgccgaga tggcccattc cggtgctgac atcggcgcac gcgaagagct gccgcccgag
     1081 gtgctcgccg atatcgaacc tgaagccgcc ccggcgaccg cctgaaccat ttttcaggag
     1141 actaaggata tggccgcgat caccgccgcc ctggtgaaag acctgcgcga gaagaccggc
     1201 gcgggcatga tggactgtaa gaaggcgctg accgagaccg atggcgatgt cgaagtcgcg
     1261 atcgactggc tgcgcaagaa gggcctcgcc gcggccgcga agaagtccgg ccgtgtcgca
     1321 gccgagggct tgatcggggt cgcctccggc acgaacaggg ccgccatcgt cgaggtcaat
     1381 gccgaaaccg atttcgtcgc gcgcaatgac acgttccagg aattcgttgc gaagatagcg
     1441 gaaatcgcgc tggaaatcgg cgacgacctc gacgcgatca acgccgcgcc gttcgccccg
     1501 ggtggccatt cggtcgccga acaacttacc cacatggtcg cgacgatcgg cgagaacatg
     1561 accatccggc gcgccaaggt gctgtcggtc cctcagggag tcgtcgccag ctacatgcat
     1621 caggcgttga agcccggcct cggcaagatc ggcgtcctgg ccgcggtcga ggggcccggg
     1681 gaacttgctg cactggaact gctgggccgt caggtgggca tgcacgtcgc cgccagccgc
     1741 cccgaagcgc tggatatcga cgcagtcgat ccggccgccc tggaacgcga gcgggccgtg
     1801 ctcagtgaac aggcacgtgc gtcgggcaag cccgagaaca tcatcgagaa gatggtcgat
     1861 ggccgcattc gcaaatacta cgaggaagtg gttctgctgg aacaagtttg ggtccatgac
     1921 ggcgaaagcc gggtgcgggc ggtggccaag aaagccggcg tcaacctgac gggcttcgtc
     1981 cgcttccagc tgggcgaagg gatcgaaaag ccgcagggac cggacttcgc agcggaggtc
     2041 gcagctacgg ctggcgtctg atatactagc cggccgaccc gctaaaacgc cgagacgcga
     2101 cttgttcaga acgggtggcg ccactagggt gccgcccgtt cttcattgcc ctacggagtc
     2161 gcatgagaca gcccgcggtt taccagcgtg ttctgctgaa attatccggc gaagcgctca
     2221 tgggccggcg cgagtacggc ctggacaccg acatggtcaa cgctattgcc agcgacatca
     2281 aggcggtcgc cgccatgggc gtgcaagtgt cggtggtgat cggaggcggc aatatcttcc
     2341 gtggcgtgtc cggtgccgcg tccggcatgg atcgcgccca ggccgattac atgggtatgt
     2401 tggcaaccgt gatgaacgcg cttgccatgc aaaccgcact ggaaaagcgg gattgtccga
     2461 cgcgcgtgca gtccgccatt cccatggcaa ccgtttgcga gccttatatc cgccgtcggg
     2521 cggagcggca catggaaaag ggcagggtgg tgatattcgc cgccggtact ggcaatccat
     2581 ttttcaccac ggacaccgcg gccgcgttgc gcgcggcgga aatgaagtgc gacgcgttgc
     2641 tgaagggcac ccaggtggat ggcgtctatt ccgccgaccc acgcaagttt acggatgcca
     2701 cgcgctacga gcaactgacc tatcacgacg ttctcgcgaa ggaccttggc gtcatggatg
     2761 ccgctgcgat cagcctcgcg cgggaaaacc acctgcccat cgtcgtgttc aacatccatg
     2821 cgcccggtgc attcgcgcag gtcatgcgtg gtgagggtgc atttaccagg atcatcgagg
     2881 cctgaacgtg cgcggagcga cagccagatc ggcccgagcg gcaccaaatc gcaagacgat
     2941 cgtggtgtgc gagcgcccgt cgctcgagcc atgttcgcct ctgccggata gggtggccgc
     3001 gccggcttct gaaataccgc tgtctcacta atcctgaagg agttgggagg aaacgtcatg
     3061 gccaccgcgg acctgaacag cctgaagcaa gaccttactc ggcgtatgga cggttcggtg
     3121 gaaacgctgc gccgggaatt tgccggcctg cgcaccggac gcgcccaccc gggccttctg
     3181 gatccggtca aggtcgttgc ctacggaacc gagatgccgc tgaaccaact ggccaccgtc
     3241 ggtgcaccgg agccgcgcat gctgaccgtg caggtctggg acaagtccat gagcggagcg
     3301 gtggaaaaag ccatccgcga ctccggcctg gggcttaatc ccgctgccga cggcatgatg
     3361 atccgcgtgc cgattccgca actcacgacg gaacgccgta acgaactcgc caagctggcg
     3421 cacaaatacg ccgaaggtgc gaagatttcc gtgcggggcg tacggcgtga cggcatggag
     3481 cagatcaagg gatacgaaaa gaagcacgag atcggtgagg atctggccaa gaactgggcc
     3541 gaggaagtgc aaaaacttac cgaccaattc attaaaaagg tggacgaggt gctcgtcgag
     3601 aaggaaaagg acatcaagca ggtttgaagg gtttggggca caggatgcct gtcatcacta
     3661 aacgggcagc cagtgtcgtg ccagaggatg ccatgtcaac aaccgtcgtg gcatccccgg
     3721 cgatgtcgtc tgccgtcacg tctgcggttg atggcggttc ggtcattgtt gctggccaga
     3781 tcgtccccgc ccacgtcgcc atcatcatgg acggcaacgg aagatgggcg aaatcccgca
     3841 gcttgccgag gatcgcgggg catcgcgagg gatcccgtgc cgtacgacgg gcggtcgaag
     3901 cggcgatccg gtcaggggtg tcctggctga cgatctatgc gttcagcagc gagaattggc
     3961 ggcgcccggc cggtgagatc caggacctga ctggattgct gcggcgatac cttcggtcgg
     4021 agatcgccga actgaaggaa tccggcgtgc gcctgcgctt catcggcgac cgcacacgct
     4081 tcgatgccga tatccagaag gatctggcgg cggcggagcg agacacttcg gataacgcca
     4141 ggctgaacct gacgatcgcc ttgtcgtatg gcgcacgagc cgaaatcgcg gcggcggcgc
     4201 gcgcagccgt ggcggcgata cgcaccggat gtatggatga agccgaactc gacgaagatg
     4261 tgttcgcgcg gttcctggcg accgccggaa tgcccgaccc cgatctgata atccgcacct
     4321 ccggggagca gcggctgtcc aatttcctgc tctggcaggc agcgtatgcc gaactggtgt
     4381 ttctcgacgt gctttggccc gatttcgacc atgagcagtt caatgcggcg ctgacggagt
     4441 tcgctcgacg ggaacggcga tatggcgccc gtaaggattg agccgctaac agaggccccg
     4501 ccggtgatat cggcagggtc ggaccgaagc gccatcgcca acgcaacacc cggcagcacg
     4561 gcctccgccc gctggggcga cctcagggtg cgtatcctgt ccgcggcggt tctggctccg
     4621 ctggccctgg ggtgcatctg gatgggcgga atggcctttg ccgccctcgt cgccctcatc
     4681 tccgccggcc tttcgctcga gtggttgggt ttttgtcacc agagcaagaa ggccgccacc
     4741 attgtcctgt tcgctgccct gccgctgtcg gtgatgctga cagcttcagg tgcaacggtg
     4801 ccggcccttg gtctgctggc ggtcgcaatg gtcgctggaa t
//
LOCUS       DASWQN010000693         3044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2574535, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000693 DASWQN010000000
VERSION     DASWQN010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2574535"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..542
                     /locus_tag="VGC82_11770"
     CDS             396..542
                     /locus_tag="VGC82_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423990.1"
                     /translation="MPARAQAGRMIKIGGKLPINLLIGAYHNLVRPEFGATWQLRTQI
                     AFIF"
     gene            complement(707..1618)
                     /locus_tag="VGC82_11775"
     CDS             complement(707..1618)
                     /locus_tag="VGC82_11775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0423991.1"
                     /translation="MIDVISGGRLIAGFPVGTPMDGCFAYGQNPSHLRERYLEAHDLV
                     MKAWQEKDTFAFNGRFNQQRYVNIWPRPIQQPHPPIWIPGGGSVETWQWCAEMDYVYC
                     YLSYYGHKIGKATMDGFWAEMDRLGKDRNPYRAGFAQTVCVAESRDQAMDLYLEAAEY
                     FFGRCLHIDPRFAAPPGYTTEATMRFGLASQVSAAAAKSVARPTEMKDLVDAGYIVIG
                     TPAEVTEQIVDLAENLNVGNLMLLLQFGNMNKDLTRYNTKLFAEKVMPTLKKFYSEWE
                     HRWWPKPMDARLRADIPAYTPTMRAAE"
     gene            complement(1615..1959)
                     /locus_tag="VGC82_11780"
     CDS             complement(1615..1959)
                     /locus_tag="VGC82_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423992.1"
                     /translation="MKLMWFHLMPYTELPDDFKQAHPSIWVDIHSSLFDPKRAHLMYN
                     DFMDELEYAADCGYDAICVNEHHSNGYGLMPSPNLIASSLARRTTDATICVMGNSLAL
                     YNPPTRVPRNSP"
     gene            complement(2802..2990)
                     /locus_tag="VGC82_11785"
     CDS             complement(2802..2990)
                     /locus_tag="VGC82_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423993.1"
                     /translation="MDYRDPPPLDPEPDIDPPPLEPLPDIDPPDISFMLPELPEDMPE
                     LPEDMPEPPEDMPPPLPL"
BASE COUNT          609 a          912 c          919 g          604 t
ORIGIN      
        1 ttcatcctga tgatgcggat atattggccc aaagaaaccc ctccctcgat tctcaatggg
       61 acttggtcgc cgccgcttgt taagaaagct ggctgagacg acaggactcg cagcacgcgc
      121 ggaagcagtg cgtgctgcat aagtcctatc ggttggtaaa tcggaatatt cgtgaagtcg
      181 cctttacgcg catgaagtat ttctggcgtt cgacgaggtg gtgctacgta aacgctcgac
      241 gacggcttta cgatcaggca tagacggaag cgcaatgact ggcggcgggg cagccgtgga
      301 ctgtggatcg cacgcccgaa gcgaaagctg gtttgttggc actgtcccgg tcatcaccgc
      361 cacctggccc gcccccgggc aacaaggctt ggaccatgcc tgcgcgcgcg caggccggcc
      421 gcatgatcaa gatcgggggc aagcttccga tcaacctgct tatcggcgct taccacaact
      481 tggtgcgtcc agaattcggg gcgacctggc agctgcgcac gcagattgct ttcatattct
      541 gattcatcgc cgcaccatcg tggtgtgtgc cgacgtgatc tgaatgcctc ggtctgggcc
      601 gtcgtgttgt gtcgcatggc tgcattacgc tagatcatta tcttgccgct gacgctggat
      661 cgccaagtca agcgtcgaac cttggcactc agcacaaaaa ccaccgctac tcggccgcgc
      721 gcatcgttgg cgtataggca ggaatatccg cacgcaaccg ggcatccatc ggcttcggcc
      781 accaccgatg ctcccactcc gaatagaatt tcttcaacgt cggcatcacc ttttcggcga
      841 acagcttcgt gttgtaacgc gtcagatcct tgttcatgtt gccgaactgc agcaacaaca
      901 tcagatttcc gacattcaga ttctccgcca gatcgacgat ctgctcggtc acctcagcag
      961 gcgtgccaat cacgatgtaa ccggcatcga ccagatcctt catctcggtc ggccgagcca
     1021 ctgatttggc ggcggccgcg ctgacctgac tggccaggcc gaagcgcatc gttgcctcgg
     1081 tggtatagcc cggtggcgcg gcaaaccgcg gatcgatatg caggcagcgg ccgaagaaat
     1141 actccgcggc ttcgagatac agatccatcg cctgatcgcg gctttccgcc acgcacaccg
     1201 tctgggcaaa tccggcccgg tacggattgc ggtccttgcc gagccgatcc atctcggccc
     1261 aaaacccgtc catcgtggcc ttgccgatct tgtgcccgta gtaggacagg tagcagtaaa
     1321 cgtagtccat ctcggcgcac cactgccacg tctcaaccga accgccgccc ggaatccaga
     1381 tcggcggatg cggctgctgg attggcctcg gccagatgtt gacgtaacgc tgctggttga
     1441 accggccgtt gaaggcgaag gtgtctttct cctgccatgc cttcatgacc aggtcgtggg
     1501 cttccagata gcgctcccgc aggtggctgg gattttgccc gtaggcgaaa caaccatcca
     1561 ttggcgtgcc aacgggaaag ccggcgatca gccgtccgcc ggaaattacg tcgatcatgg
     1621 cgaattcctc ggcacccgcg tcggtggatt gtacagcgcc agagaattgc ccatcacgca
     1681 gatcgtggca tccgtggtgc gccgagccaa cgaggacgcg atcaggttcg gcgacggcat
     1741 caatccatag ccgttggagt gatgctcgtt tacgcagatt gcgtcgtagc cgcaatcggc
     1801 ggcgtattcg agttcatcca tgaagtcgtt gtacatcagg tgcgcgcgct tcgggtcgaa
     1861 cagcgacgag tggatatcga cccagatcga cggatgcgcc tgcttgaagt cgtccggcag
     1921 ctccgtgtac ggcatcaggt gaaaccacat cagcttcatg ggcgctcctc gtcgtgatca
     1981 ggctaccgat cggtagcctg ctccggctcc ctgctgcgat caagccgtca ttttgcggcc
     2041 tccgcttgtg gcttcttctc tggcgtttgc ggtttgggcg ccggcttcgg cggtatgccg
     2101 gctggcccgg ttatcacatc gaaatcgaaa ctgctgcgtg gcatcggttg ctccttggtt
     2161 ggtggaccaa agaccgttcc agtcttagcg gagttcgatt catgagacag aaaaacgaaa
     2221 gccgcctggg tggggcggct ttggatttgg ctacgactgc acgatcctaa accgccggga
     2281 cataccagcg gtaccaaaat cgcgaagcgg gtgaagtcgt aaccatacga gttacataca
     2341 gccttcatgg gctgggcgca acacgaggaa acgtggcgga cgggcaaccg gacctgatcg
     2401 tcccgcgttc cggaagtcgc accacgtccc gtggaggccg ggagcaccga tccgcctccc
     2461 ggcgaagcca ggtaggagat gaaaatgtcg aagacgcgtt tgcttcaggc ggtttgcaca
     2521 gttgcgttgc tggcggcggc gccggcgttt gctcaaacca atatccagcc cgccgatacc
     2581 ggcgtcggcg gcgcacccaa taatcccacc gcaaatgaga ccatgccgaa ccggtcaggc
     2641 agcatggcac cggccgatcg catgggggcc atgggctcgc attccggaat ggatagccat
     2701 tcgacgaggc atgcggcgat ggatcaccga ggcaggtccg cggatacaag cgccgatcaa
     2761 ctgaacgaac aaagctacca agccgcacgc agcggtcagg cctacaacgg cagcggcggc
     2821 ggcatgtcct cgggcggctc gggcatgtcc tcgggtagtt cgggcatgtc ctcgggtagt
     2881 tcgggcagca tgaatgatat gtccggagga tcgatgtcag gcagcggctc aagcggcggc
     2941 ggatcgatgt ccggctccgg atcgagcggc ggcggatcgc ggtaatccaa cgataaaacg
     3001 tcgcggcatc gctgcagggc ggtgccgcga cgccttcaat agac
//
LOCUS       DASWQN010000694         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_67587, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000694 DASWQN010000000
VERSION     DASWQN010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67587"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..600
                     /locus_tag="VGC82_11790"
     CDS             91..600
                     /locus_tag="VGC82_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HEY0423994.1"
                     /translation="MTAAPKPPSGQEGFFWKTKTLEEMSDSEWESLCDGCGRCCLEKL
                     EEEDTGKIYFTHVSCKLLDAGLCACKDYPNRSDQVSDCVRLTPDNVRTLNWLPPSCGY
                     KLVAEGRDLYWWHPLISGDPDTVHEAGVSVRGRVQGTETEIADADLEDHIVQWPRLLP
                     KRARLKKRP"
     gene            816..>1896
                     /locus_tag="VGC82_11795"
     CDS             816..>1896
                     /locus_tag="VGC82_11795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0423995.1"
                     /translation="MPEAVADDLSKLPAQVIELSDASIAPRPALKPNEVRTILMSLML
                     TMFLAALDQTIVATALPTIGRQFQDVSSLSWVITAYLLASTAVAPVFGTLSDIYGRRA
                     MITTALSLFIAGSILCALAPNMPVLIVARGLQGLGGGGILPVVQTVISDVVTPRERGQ
                     YQAYFSGVWVAAGIGGPVLGGVFAEHLHWSMIFWINVPLGVASLALLLPKMGKLPVFH
                     RRRQVDWLGGVLLMASAVVFMLVLTWGGNRYLWLSPVIMAMVGSSVALALAFVWHARN
                     ADEPFLPLPLLGGTVVPYAILAGSCALGAMVGLTVHLPLYYEVVYHLSASEAGLALIP
                     LAAISTVGAAIAGRTMARAKRYKRVA"
BASE COUNT          313 a          613 c          609 g          361 t
ORIGIN      
        1 tgcaggtcac tgacacaccc cacttgcacc gttgacgtcg caccccttag aaagggcgga
       61 attcaccgtc cccggaagcc cccaggatcc atgaccgcag ctcccaagcc gccctcggga
      121 caagagggat tcttttggaa aaccaagacg ttggaggaga tgtctgactc cgaatgggaa
      181 agcctgtgcg acggttgcgg gcggtgctgc ctggagaagc tcgaggagga ggacaccggc
      241 aagatttatt ttacccatgt ttcctgcaag cttctggatg ccggcctatg cgcctgcaag
      301 gactatccga atcggtccga ccaggtttcc gattgcgtcc gccttacgcc ggataacgtc
      361 cgcacgctga actggctgcc gccgagctgc ggctacaagc tggtggccga ggggcgcgat
      421 ctctattggt ggcatcccct gatctcgggc gatcccgata ccgttcacga ggccggcgtt
      481 tccgtgcggg ggcgcgtgca gggcaccgag accgagatcg ccgacgccga cctcgaagac
      541 cacatcgtgc aatggccccg gttgctgccg aagcgggcgc gcctcaagaa gcgcccataa
      601 tcccgacttg cgccatcacg cgattgcgtt gccccctgtg cgggaagcac ccatgcgcgg
      661 aacccgctgc cgcggcttgg ccttgccgcc taaattgcgg cggtcgagcg attcgacatc
      721 gccatctcgg ccctcccgtc gcgcttcgca gataaataaa catcgccatg aacaagttcg
      781 aacggcagag ccgtcaaccg gccgattcac aaccattgcc cgaagccgtc gccgacgacc
      841 tgtcgaaatt gccggctcag gtcatcgaac tcagcgacgc gtcgatcgcg ccgcggccgg
      901 cgctgaaacc gaacgaagtc cgcaccatcc tgatgagcct gatgctgacg atgttcctgg
      961 ccgcgctcga ccagaccatc gtcgcgacgg cgctgccgac cattggacgg caatttcagg
     1021 acgtctccag cctgtcctgg gtgatcaccg cctatctgct ggcctcgacc gcggtggcgc
     1081 cggtgttcgg caccctcagc gacatctatg gccgccgcgc catgatcacc accgcgctca
     1141 gcctgttcat cgccggctcg atcctgtgcg cgctggcgcc gaacatgccg gtgctgatcg
     1201 tggcgcgcgg cctgcaggga ctcggcggcg ggggtatcct gccggtggtg caaaccgtga
     1261 tctcggacgt ggtaacgccg cgcgaacgtg gccagtacca ggcctatttc agcggcgtat
     1321 gggtggcagc gggtattggc ggaccggttc tcggcggcgt gttcgccgaa catctgcact
     1381 ggtcgatgat cttctggatc aatgtgccgt taggcgtcgc atcgctggcc ctgctgctgc
     1441 cgaagatggg caagctcccg gtgtttcacc gcaggcgcca ggtcgactgg ctcggcggcg
     1501 tgctgctgat ggcctccgcg gtcgtcttca tgctggtgct gacctggggc ggtaatcgtt
     1561 atctctggct gtcgccggtc atcatggcga tggtgggatc gtccgttgcg ctggcgctgg
     1621 ccttcgtgtg gcatgccaga aacgccgacg agccgttcct gcccctgccg ctgctgggcg
     1681 gaacggtcgt tccctacgcg atcctggcag gcagctgcgc gcttggcgcg atggtcgggc
     1741 tgaccgtgca tctgccgctg tattatgagg tggtttatca tctcagcgcc agcgaggcag
     1801 ggctggcgct gattccgctg gcggccattt caacggtagg ggccgcgatc gccggccgga
     1861 ccatggcgcg cgccaagcgt tacaagcggg tggcga
//
LOCUS       DASWQN010000695         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1914804, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000695 DASWQN010000000
VERSION     DASWQN010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914804"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VGC82_11800"
     CDS             <1..197
                     /locus_tag="VGC82_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423996.1"
                     /translation="MSEHTEQTDDKDAEKAAHAERDAKVDDAVEDTFPASDPPSTTPP
                     PGTRKAEQIEAEQKSEAENE"
     gene            complement(356..1645)
                     /locus_tag="VGC82_11805"
     CDS             complement(356..1645)
                     /locus_tag="VGC82_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0423997.1"
                     /translation="MRWKAAQTYESAPYSMQDRNAMLSTRRTILSATAAATILPRISR
                     AQPPTIRIGVLNDQSGPYRDDGGPTGVVCAKQALEDFGLSGKGWNVEVLSADHQNKPD
                     VGISIARQWFDRDGVDAIVDVPTSSVGLAVNTICREKNKVYLNSGTGSSDLTGPQCSP
                     NTVHWTYDTYMLGKSTGGATVKAGGDTWFFITADYVFGQTLQRDTAAVVTKAGGKVLG
                     SIAYPFPETTDFSTQLLRAQASGAKVLGLCMAGADTENCIKQAHEFGLTRSMALTALL
                     IQVRGVRALGLETAQGLHWSESYYWDLNDRTRAWNDRVRTKTPNGVWPNMTQAGDYAV
                     TLHYLKAVAEMGVAAAKADGRAVVQRMKAMPTDDDCFGPGRIREDGRALHPVYLLEAK
                     TPSESKQSWDVAKVIATTPMEEAFRPLNEGGCPLVKS"
     gene            complement(1691..2020)
                     /locus_tag="VGC82_11810"
     CDS             complement(1691..2020)
                     /locus_tag="VGC82_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0423998.1"
                     /translation="MYPPATREMAQQRRELAPAAQAAFDAFGKAAFADGALSAKMKQI
                     IAVAAAHVTQCPYCIKAHTKAARRAGATPQELMEAIWVAAEMRAGAAYAHAALMLDTL
                     EAESERH"
     gene            2321..>2585
                     /locus_tag="VGC82_11815"
     CDS             2321..>2585
                     /locus_tag="VGC82_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0423999.1"
                     /translation="MKTIPIGSKGTAGLLVTPAHLANQVKDGTLPPVFATPMMIVVME
                     NAALNAIRPFFDEGESAVGTAVNVQHLAATPVGHQVRAEAEVIG"
BASE COUNT          496 a          782 c          795 g          512 t
ORIGIN      
        1 taatgtcgga acataccgaa cagactgatg acaaggatgc tgaaaaggcc gcccacgctg
       61 agcgcgatgc caaggtcgat gatgcggtcg aggatacctt ccctgccagc gatccgcctt
      121 cgacgacacc gcctcccggc acgcgcaagg ctgaacagat cgaggctgag cagaaaagcg
      181 aagccgagaa cgaatgagcc gtaggttcta cgtttaacac tgagctttgt gtaatcctgt
      241 tggacgacgt tgcggccgcc cgggcaatgg ccacctaatt tcggaactat gcctggatgt
      301 gctccgaagc tcatgcgtta gacgcagacg tggtttagag ccgaacgcgc ctcaatcagc
      361 ttttcaccag cggacagccg ccttcgttca gcgggcggaa ggcttcctcc attggcgtgg
      421 tggcgatcac tttggccaca tcccaagact gcttgctttc ggatggcgtc tttgcctcca
      481 gcagatacac cggatgcagc gcgcggccat cctctcggat cctgcccggc ccgaagcagt
      541 catcatcagt tggcatggct ttcattcgct ggaccacggc acggccgtcc gccttcgccg
      601 ccgcgacgcc catctcggca accgccttca ggtaatgcag cgtcacagca tagtcgccag
      661 cctgggtcat gttgggccat accccgttcg gcgttttggt ccgcacgcgg tcattccagg
      721 cccgcgtgcg gtcgttcaaa tcccagtaat aggactcact ccagtggagt ccctgcgccg
      781 tctcaagccc gagcgcgcgc acaccgcgca cctggatcag cagggcggta agcgccatgg
      841 agcgggtaag gccaaactca tgggcctgct tgatgcagtt ctcagtgtcg gcaccggcca
      901 tgcacaggcc cagcaccttg gcgccggagg cctgggctcg gagcagctga gtcgaaaagt
      961 cggtggtttc cgggaacggg taagcgatgc tgcctagcac cttgccaccg gctttggtca
     1021 cgactgccgc ggtgtcacgc tgcagggttt ggccaaacac ataatcggcc gtgatgaaga
     1081 accaggtgtc accgcctgcc ttcaccgttg ctcccccggt ggacttgccc agcatgtagg
     1141 tgtcgtaagt ccagtggact gtgtttgggc tgcattgcgg cccagtcaaa tccgaagatc
     1201 cggtgccaga attgagataa accttgttct tctcccggca gatcgtattg accgcaaggc
     1261 cgaccgagga ggttgggacg tcgacgatgg cgtccacccc gtcgcgatcg aaccattgcc
     1321 gcgcgatgga gatcccgaca tctggcttgt tctggtgatc ggctgacagc acctcgacat
     1381 tccagccctt gccggaaagg ccgaaatcct ccaacgcctg cttggcacag acgacaccgg
     1441 tcggaccgcc atcatcgcga tacggtccgg actgatcgtt caacacgcca atacggatgg
     1501 tggggggttg cgcacggctg atccggggca agatggtggc cgcagcggtt gcactgagga
     1561 tggtgcgtcg ggttgatagc atggcattcc tgtcctgcat tgaatatggc gccgattcgt
     1621 aggtctgtgc cgccttccac ctcatccagc acgatttgct gtgtgttctg cgtgggtggg
     1681 ggcggccggt ctagtgacgc tcagactccg cttcaagcgt atccagcatc aatgccgcat
     1741 gcgcatacgc ggcgccggcg cgcatttctg ccgctaccca gatggcctcc atcagttcct
     1801 gcggcgtcgc accggcacgc cgcgcggcct tggtgtgagc tttgatgcag tagggacatt
     1861 gagtgacgtg cgcggccgca accgcgatga tctgtttcat cttggctgac aaggcaccat
     1921 cggcaaacgc cgccttgccg aaggcgtcga acgcggcctg ggcagcgggt gcaagttcac
     1981 ggcgctgttg cgccatttca cgagtggcgg gcggatacat gcgatcaggc atcctttctc
     2041 cttcgggacg gtccaatgct ttcgcggccg tcctgtcaac cgagcattgg tgacgagacc
     2101 gagaaacccg cagcgccgct caggttgcaa tgagggcatt gtcaacttaa tcggctgtag
     2161 acgcgttgat acccggaccg gattgagcac gtgaatcgct atgccagagc ggcaacatcg
     2221 gtgaccgcgg cccacctctg gaaggcttgg tgcggatggc gctcgcaaac gctgcgggag
     2281 ccaagcaacg atggtatgca agcaaccgta aggacagttc atgaaaacga ttccgatcgg
     2341 gagcaagggc actgccggac tgctcgtcac cccggcccat cttgcgaatc aggtaaagga
     2401 cgggacgctt ccgccggttt tcgcgacgcc gatgatgatc gtggtcatgg agaacgccgc
     2461 gctgaatgcg atccggccgt tcttcgatga gggcgagagc gctgtcggca ctgcggtgaa
     2521 tgtgcaacac cttgccgcca cccctgtcgg ccatcaggtc cgtgcggagg ctgaagtgat
     2581 tggag
//
LOCUS       DASWQN010000696         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2046796, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000696 DASWQN010000000
VERSION     DASWQN010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046796"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            823..1044
                     /locus_tag="VGC82_11820"
     CDS             823..1044
                     /locus_tag="VGC82_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424000.1"
                     /translation="MEQPGLDQRHRDKNGQIARKHGNTLISSLRQVYGAHFAADCPGN
                     AKLSDVLHQLDEPSLTKLVHDVHGHEAGD"
     gene            1562..>1692
                     /locus_tag="VGC82_11825"
     CDS             1562..>1692
                     /locus_tag="VGC82_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HEY0424001.1"
                     /translation="MQALVWHGKEDIRCDRVSDPGIEHPRDAIVKITSCAICGSDLHL
                     "
BASE COUNT          327 a          490 c          559 g          316 t
ORIGIN      
        1 agttccatga ccttggcgag gtcggtcccc aacgcgtcgc tcacaagccg gcaagcctcg
       61 tgcaggatcg agccgatgtc ctcgtatttg atggccaact caccaaaccg cgcaagcaca
      121 ttttgctgcc gcaggagctc agcttcgcgg gttgacgcca ctgcggcgga ctcctgtatg
      181 ggtgcgaccg gtggcaaccg tgctcatcgt cgctgatgcg tcacgccact ggctgatggg
      241 tcatgcctgc ccaggcccgc ggcgtaggca ggctgtctgc tggccaagcg ataacgatca
      301 cgccgatagc gctgcgagcg ggcccgcggc tttggatctg ccacgggccc gcggtccggg
      361 aactgcgtcg aatacagtgg ccgaccccac gaactcatgg gtcggccggt gccggttggc
      421 tgccttagcg gtgggtggtc gctgtcgaat gcgagctccc acggttgaac aggtaatggt
      481 cggccgacaa ggcaagaagc ggcaggatga tcaaggtaag cgcgaagtac atcggaattt
      541 tccgttctca ggttggtgaa aatcgaacac cccatgaaga gtggcgttcg catgtcgctt
      601 cgagttgatc gagccggttc ggctgccgcg acgcacagcg gtggcaggat gctaaaaaag
      661 agagggttcc ccgggtttcg ctggggggcg gcgcttgggt cgaggcaaca cctttttggc
      721 gcattggccc gatccgcggg cttgacgggc tttggcggcg acaagcgcgc cggatgcaac
      781 ggccagccgt cggcggcgtt ctcgggtaaa ggaggacagc agatggaaca gcctggcctg
      841 gatcaacgcc accgcgacaa gaacggccag attgcgcgga aacacggcaa cacgctgatt
      901 tcgagcctcc gccaggttta tggcgcccat ttcgccgccg actgtcccgg gaatgccaag
      961 ctgtccgacg tgctgcacca gctcgacgag ccatccctga cgaagctggt tcatgatgtt
     1021 catggccatg aagctggcga ctgagactgg tggtgaaggt ttagagtcca ttaccgaata
     1081 ccgcttcagg tgcggcctcc cgctccgtcc tgggggcgag aatcaccgcc gctaaatggg
     1141 cttccagcgc gggccatggt gccactgcgg gaaaatcgtg gaaccggcaa cgaaccggcc
     1201 attaccttgg gcggcccgac tccgccgggc ggaccaattt gacgcaaatg gcgcgcgcgg
     1261 gtggcatagc gttgctggcg ggactacccg gcctgaagcg gtatttgtca tcaactactg
     1321 gtttatagta acgacaagcc aggttcggct acgtgctgaa caccaggggg gcggttttcc
     1381 tgtggtacct gcgcctatgg gccgcgaccg cggtacctcc agaacgtgat agctggacag
     1441 cgaacgcgtc ccatttctca aggttgttgg ctcccagata gtaaatcgac ggaaggttcc
     1501 aaaggcgcgg ccgttggggc gcggccattg tggcaggaca gcagaattgg ggagcgcagg
     1561 catgcaggct ttggtgtggc acggcaagga agacatccga tgcgaccggg tttcggatcc
     1621 cgggatcgaa catccgcgcg atgcgatcgt gaagattaca agctgcgcca tctgcggctc
     1681 cgaccttcat ct
//
LOCUS       DASWQN010000697         1914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1255169, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000697 DASWQN010000000
VERSION     DASWQN010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255169"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..516
                     /locus_tag="VGC82_11830"
     CDS             142..516
                     /locus_tag="VGC82_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0424002.1"
                     /translation="MVAHLVLALDAQRKWCRSRGMPFPPALAALLDSLAVELRRNRPE
                     AARRGPDRSDVGDVPISAEAEPVPQPLCIDDRAAALLLGLSERTVRRLRASGVLPSVV
                     AGRRRLIRLTDVEAFVANGGTQ"
     gene            513..1460
                     /locus_tag="VGC82_11835"
     CDS             513..1460
                     /locus_tag="VGC82_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424003.1"
                     /translation="MTEYKAFIPLSFKSTGQGSATFVIAHTGVLDLDGDILVPGAIGQ
                     QVATVLPAHNWNSLPLGKARISEQGNQVVADVQFNPTPDGQAWYEAIKWDFENPPSVQ
                     QYSWGYTPTKSRPGEHNSQPVRFLESVVIHECSPVVVGASIGTHTAGVKQRDILSSSE
                     WAIIRAARHEADAVEAEMKTKVRSIAKAFHATEESLYADRVAVESNEAMGHITTVVPA
                     ATVSTAVRSAAQVATKLAAAQLGTKTPEIRWFSEPDEVTLYGRALPSRDIIYLNDRLT
                     QTQSLVIAAHEVGHCAGLTETECRAYEGHFLERTLRDTL"
     gene            1447..1680
                     /locus_tag="VGC82_11840"
     CDS             1447..1680
                     /locus_tag="VGC82_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424004.1"
                     /translation="MTLYSGRATTGREATYAEKEASLLEALELEFLDECHPNTADILK
                     RRLLPLESDHLLMVLELIVVAKENYASRMASAS"
     gene            1677..>1914
                     /locus_tag="VGC82_11845"
     CDS             1677..>1914
                     /locus_tag="VGC82_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424005.1"
                     /translation="MTAKTARCHLCGRIVGRKSCRQEDSGWYCVQRGLLVGVGVEIIT
                     PQPIPQPWKDDPALLAATAAEEQAQQTYDRADMEW"
BASE COUNT          429 a          617 c          555 g          313 t
ORIGIN      
        1 taacggatcc ccacgcgtca accgcgctcg acacgatgac cggctggaac accgtggata
       61 actattcggc cacccccgcc gacatcgcag agagcgcacc atcaccatca cagcgccggt
      121 atgaacggcg acggtgatca cgtggtcgcg cacctcgtcc tggcattgga tgcccagcgc
      181 aaatggtgcc gatcccgggg gatgccgttt ccgccggctt tagcggcgct gcttgattcg
      241 ctggcggtcg agcttcgtcg gaatcggccg gaagcggcca gacgcggacc ggacaggtca
      301 gacgttggtg acgtgccgat ctcggcggaa gctgagcctg tgccgcaacc attatgcatt
      361 gacgaccgcg ccgcggctct cctgctcggt ctcagcgagc gcacggtgag gcggttacga
      421 gcgagtgggg tgctgccgtc ggtcgtggct ggccggcggc gtctcattcg ccttaccgat
      481 gtggaggcct tcgtggcgaa cggcggaacc cagtgacaga gtacaaggcg ttcatcccct
      541 tatcttttaa atcgacagga caaggcagtg ccacattcgt catcgcccac accggggtgc
      601 tcgacctaga tggggacatc ctagttccgg gtgcaatcgg tcagcaggtg gccacggttc
      661 taccggccca taactggaat agtttaccgt tggggaaagc gcgaatcagt gaacagggca
      721 accaagtcgt cgccgacgtc cagttcaatc cgacgccaga cgggcaagcc tggtacgagg
      781 caatcaagtg ggactttgag aatcctccct cggtgcagca gtacagctgg ggatacaccc
      841 ctaccaagtc gcgcccaggc gaacataaca gccaaccggt ccggttcctt gagagcgtgg
      901 taatccacga atgcagcccg gtggtggtcg gcgccagcat cggcacgcac accgcgggag
      961 taaaacagcg cgatatactt tcatcgtccg aatgggccat catccgcgcc gcgcgacacg
     1021 aggcagacgc cgtcgaggcc gaaatgaaaa ccaaggtccg atccatcgcc aaagcgttcc
     1081 atgccaccga agagtcgctg tacgccgacc gggtcgccgt cgaatcaaat gaagcaatgg
     1141 ggcacatcac gaccgttgtt cccgcggcca cagtctccac agcggtccga tccgccgccc
     1201 aggtggccac caaactcgcc gccgcacaac tgggcactaa aacacccgaa atccggtggt
     1261 tcagcgagcc tgacgaggtc accctctacg gtcgagctct gccatcccga gacatcattt
     1321 acctcaacga tcgattaacc caaacgcaaa gtctagtgat cgcggcgcat gaggtcgggc
     1381 actgcgccgg actcaccgaa acagaatgcc gcgcatacga aggccacttc ctcgaaagga
     1441 cgctccgtga cactctatag cggcagagcc accaccggtc gcgaagccac gtacgccgaa
     1501 aaagaggcta gtctcctgga ggcgcttgaa ctggaattcc tcgacgagtg ccacccgaac
     1561 accgctgaca tactgaaacg gcggctacta cctctcgaat cggaccacct cctcatggtc
     1621 ctcgaactga tcgtcgtggc caaagaaaac tacgcgtcgc gaatggcatc ggcatcgtga
     1681 ccgcaaaaac agcccggtgc catctatgcg gccgcatcgt cggccggaaa tcctgtcgac
     1741 aggaggacag cggatggtac tgcgtccagc gcggtctact ggtcggtgtc ggcgtcgaaa
     1801 tcatcactcc acaaccgatc ccccaaccct ggaaagacga cccggcactg ctagccgcca
     1861 ccgccgcaga ggagcaggca cagcaaacct acgaccgtgc cgacatggaa tgga
//
LOCUS       DASWQN010000698         1685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1651042, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000698 DASWQN010000000
VERSION     DASWQN010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651042"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..690
                     /locus_tag="VGC82_11850"
     CDS             118..690
                     /locus_tag="VGC82_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0424006.1"
                     /translation="MGGSGWFGTVGAGYDVQFGGWVAGIFGDGQFGDLRGSLIDSQTG
                     TEGRERLRTSYAAGARLGYLVAPNVLSYANAGYSGSEWSGTTQAFPNVSALRPVVSTT
                     PSFRRDGWFIGGGVENSLNFFGITAPGWFMKTEYRSAYYDRISLPETYVPGNIYSSTG
                     LTGTAVTFKPWVQTISTSLVYRFNWAGSRY"
     gene            861..1046
                     /locus_tag="VGC82_11855"
     CDS             861..1046
                     /locus_tag="VGC82_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424007.1"
                     /translation="MRKLLLVVGLFTLAIGLLWIGQGTGVIDWPQSSFMIRQIQWAGY
                     GAALAALGLVLIWQSQR"
     gene            1165..>1685
                     /locus_tag="VGC82_11860"
     CDS             1165..>1685
                     /locus_tag="VGC82_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0424008.1"
                     /translation="MTTALFDLRGKVAIVTGGNGGIGLGMARGLAEAGADIAIVGRNE
                     AKSTAAVAELKRHGVRAMAATADVTDKAAVADMVARTASELGRIDILINNAGINIRKP
                     PHALEIEEWESVIRTNLTSAFLCSQAVYPVMKQAGGGKIINIGSMMSIFGASFAPAYA
                     ASKGGIVQFTRSCA"
BASE COUNT          290 a          523 c          547 g          325 t
ORIGIN      
        1 ggcggcggtt tgtggaatgc cgacagcggt gtgcagagca cgggcgttgc tgttccaggc
       61 cccctcggag cttctacttt tggatcggcc ggcacggcgt taatccggga tcaacgcgtg
      121 ggtggcagcg gctggttcgg aaccgtcggt gcgggttacg acgtgcagtt cggcggctgg
      181 gtagctggca ttttcggcga tggccagttc ggcgatcttc ggggctcgct gattgattcg
      241 cagaccggaa ccgagggtcg tgaaaggctt cggaccagct acgcggccgg cgccagacta
      301 ggctacctgg ttgcgccaaa tgtgttgtcc tacgcgaatg ccggctactc cggctcggaa
      361 tggtcaggca ccacccaggc gtttccgaac gtcagcgccc tcaggcctgt ggttagtacc
      421 acgccgtctt tccgtcgtga tggctggttc atcggcggcg gtgtcgagaa cagcctgaac
      481 ttcttcggca tcacggcacc gggctggttc atgaagaccg aataccgctc cgcgtactat
      541 gaccgcattt cactgcccga gacgtatgtt ccgggaaata tttatagttc cacgggtcta
      601 acgggcactg cggtcacctt caagccctgg gtgcagacca tcagcacctc gctggtctac
      661 cgcttcaact gggccggctc gcgctactga tctcctccga aatccagcga ctcgaaagcc
      721 ccggcatagt ccggggcttt tttgtaagtt ggcgggatag ctccaggcct cgggcgactc
      781 gaggtccttg ccttgggtcg accggcacgc cagtctgacc tggtttcgac gttaccgcgc
      841 gccccaaacg ggaagcccca atgcgcaaat tgctactggt cgttggtctg ttcacgctcg
      901 cgataggcct gctgtggatc ggccagggca ccggtgtgat cgactggccg caatccagct
      961 tcatgatccg gcagatccag tgggccggtt acggcgcggc gctggccgcg ctcggcctgg
     1021 tcctgatctg gcagagccag cgctgatcga agcggccaga ctcggcacgt cgcgcgtccg
     1081 tcttgtccgg gccgttcgcg tctcctaaca cttgcagccg gcatgaacgg cccgggaccg
     1141 ggcacggcag gaggaacacc ggcaatgaca acggcgcttt tcgatctccg cggcaaggtc
     1201 gcgatcgtca ccggcggcaa tggcgggatc ggacttggca tggcgcgtgg gctggccgag
     1261 gccggcgctg atatcgccat cgttggacgc aatgaagcaa agtcgacggc ggcggtcgct
     1321 gaactgaagc ggcacggcgt cagggcaatg gccgccaccg ctgatgtcac cgacaaggct
     1381 gcggttgccg acatggtcgc gcggacggcg agcgaactcg gccggatcga catcctcatc
     1441 aacaatgccg gcatcaacat tcgcaaaccg ccccacgcgc tcgagatcga ggaatgggag
     1501 agcgtgatcc ggaccaacct caccagcgcc ttcctgtgtt cccaggcggt gtaccctgtc
     1561 atgaagcagg cgggcggcgg caagatcatc aacatcggtt cgatgatgtc gatcttcggc
     1621 gccagtttcg cgccggccta cgccgccagc aagggcggca tcgtgcagtt cacccgctcc
     1681 tgtgc
//
LOCUS       DASWQN010000699         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1783129, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000699 DASWQN010000000
VERSION     DASWQN010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783129"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VGC82_11865"
     CDS             <1..201
                     /locus_tag="VGC82_11865"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0424009.1"
                     /translation="DKILLRKRFVIETVLGILKSEMGLEHSRHRSPVNAMVHVLSCLV
                     AYAFRPGKPSISLKAKQLQAYP"
     gene            262..1389
                     /locus_tag="VGC82_11870"
                     /pseudo
     CDS             262..1389
                     /locus_tag="VGC82_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612008.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1535..1799
                     /locus_tag="VGC82_11875"
                     /pseudo
     CDS             1535..1799
                     /locus_tag="VGC82_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199072.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          352 a          550 c          562 g          335 t
ORIGIN      
        1 gacaagatcc tgctgcgcaa gcgcttcgtc atcgagaccg tgctcggcat cctcaaatcc
       61 gaaatggggc ttgagcattc acgccacaga tcgcccgtca acgccatggt ccatgtgctc
      121 tcctgcctcg tcgcttatgc cttcagaccc ggcaagccat ccatctcgct gaaagctaaa
      181 cagcttcagg cttatccata gttggggtta agtggaattt ctgcctgaga gcggcgagga
      241 tgccgcggat caggagcaag tatgaccaag cgaacacgac gaacccatgc agcggccttc
      301 aaggccaagg tggcgctggc cgcgatcaag ggcgagaaga cgctggccga gttggcgcag
      361 cagtatgatg tgcaccccaa ccggatcacg gcctggaagg cacacgtggt tgaggcggcc
      421 tcgggccttt tcggcgcggc atccgacgcg gcccggccat cgatgtgaag acgctccacg
      481 ccaagatcgg ggagctgacg ttggagaacg attttttgtc cggtgcgctc accaaggccg
      541 gcctgctgag cgcaaagcga tgatcgatcg gacccacgac cttccgctgg cacggcaggc
      601 agcactgctg cggctcagcc ggagcagcct ttactacgaa ccacaacccg cgccggagga
      661 tgaattcgcg atcatgcggc ggatcgacgc actgcatctg gattacccgt tcgcgggcag
      721 ccggatgctg cgtgacctgt tgcgcggcga aggtgtcatc atcggccgtg agaaggtgcg
      781 gacaatgatg cggcggatgt gcatcgaggc gttgtatcgc cggccgaaca ggtcaaagcc
      841 gtgtccagga cacagattta tccgtatctg ctgcgcggcc tggcggtcga gtggccgaac
      901 caggtctggg ccatggacat cacctacatt ccgatggccc gtggtttcgt ctaccttgcg
      961 gccgtggtgg actggttcag ccggcgtgtt ctggcctggc gggtttcgat caccatggag
     1021 gtcgatttct gcctggaggc ggtcgaagag gcgctggcca ggcacggcaa gcccgccatt
     1081 tttaataccg atcagggctc gcagttcacc agcgctccct tccccggcct gctgttgaag
     1141 aacgggattg cgatcagcat ggacgggcgc ggcgcctggc gggacaacgt ctttgtcgag
     1201 cgtctatggc ggtcggtgaa gtatgaagaa gtctatttgc gggcctatga aacggtcgcc
     1261 gaggcccgga ggctgatcgg gcggtatctg gatttcttca acaccaagcg gccgcactcg
     1321 agccttggtg cgcaaacgcc cgaccatgcc tactttactc agtcgccgcc gttagcagca
     1381 gccgcatgaa tttcgccgtt gctgttgggt cctctctccg gtcgggctac gccctccctg
     1441 cgcgacgacc caacagcaac acattacgca accaaccggc agggaatcca cttaacggag
     1501 gcgaaacgct gtagcgaaca agccgaccac ctctgtggtg atcccaccac gcgcgacggc
     1561 tgtccctagc agcgaaccgg gtttgccgac acagcgcgac cgccatctgg caatgatcgc
     1621 ggaacatggc cgcctggctt ggcaggccgc cacccattac ggccaacggt ccttggtaga
     1681 aacgacaatg ggtcgataca aagcgctgat cggaccccgg ctacgcgctc gcggctttgc
     1741 ggcccagcag actgaggctg ccattggcgt ggcgatgcta aaccggatgt tggcggccg
//
LOCUS       DASWQN010000700         5184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1387596, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000700 DASWQN010000000
VERSION     DASWQN010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387596"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..2096
                     /gene="ligA"
                     /locus_tag="VGC82_11880"
     CDS             12..2096
                     /gene="ligA"
                     /locus_tag="VGC82_11880"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY0424010.1"
                     /translation="MLEATARQAVETLTEAEAAEELEHLAQEIAHHDAAYHTHDAPEI
                     SDADYDALRRRNSAIEARFPELIRADSPSSRVGGAPETGFAKLRHRVPMLSLDNAFDA
                     AEFAEFCNRAKRFLGRTEPLVFVAEPKIDGLSINLTYEHGRFTRGATRGDGTEGEDVT
                     GNLRTIKSVPDRLRGEAPALIEIRGEVFMTKADFLALNQAQAEAGGKVFANPRNAAAG
                     SLRQLDVRITAGRPLSLFAYAQGESSEPVAATHWEYLQRLKDWGFEVNPLSRRLGSEA
                     DAEAFQNEIGLARSGLGYDIDGVVYKIDDLGLQRRLGFVGRAPRWATAWKFPAEQATT
                     VLRDIRIQVGRTGALTPVAELEPVNVGGVLVARATLHNEDEIARKDIRIGDTVVLQRA
                     GDVIPQIVSVVQDRRRPDQAAFVYPEVCPACGSNAVRPPGEVVRRCTGGLICPAQRVE
                     RLIHFVSRPAFDIDGLGEKTIQEFYSEGWLHGPADLFKLPEREAEIAKREGWGKLSAR
                     NLSRAIAARRTVPLERFIFALGIRRIGSTNAKLLARHYGSFANWREQMLAATTIGSDE
                     RLALGSISGIGPTIAEELTDFFGEPRNIAALDELAAELTIQDAARAEAADSPIAGKTV
                     VFTGTLETLSRPEAKARAEALGAKVTDSVSKKTDIVVVGADAGSKARKATELGVRTMT
                     EAEWQELVGDPA"
     gene            2187..2696
                     /locus_tag="VGC82_11885"
     CDS             2187..2696
                     /locus_tag="VGC82_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY0424011.1"
                     /translation="MMAKSHVVVGLATWIAAAPLLHIFPLNPAYLGLAVAGSLLPDID
                     HPKSWVGRRTWPVSTALASILGHRGVTHSALAVVGLAALLLQAGYRWGGVSALIVGYL
                     SHLAADMLTPQGLRLAWPLRGTWALPICRTGSVMEPIIVALLVCGIGWWIFRHGLGGA
                     PALPRKPSG"
     gene            complement(2913..3839)
                     /gene="phnD"
                     /locus_tag="VGC82_11890"
     CDS             complement(2913..3839)
                     /gene="phnD"
                     /locus_tag="VGC82_11890"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0424012.1"
                     /translation="MLRRQAIATGAVALGFGLGSGRRARAQTVKEFRIGILGGENTQD
                     RLTRYDEFQKLLEAHLKMPVKLFPAADYAGVMQGIAAGQLEAAEFGASGFAGAWLDCK
                     CIEPVVVPQEADGSTYYYSVMVVRADSGIKSLADMKGRSLAWADPNSTSGYLIPSATL
                     KTKGIDLKDGGYFTKTGFSGGHEQGVVAVLNRQYDAAVTWTSGQGEKSEGYTRGNLRS
                     MVDRKMLKMSDIDIIWQSGKIPNGPWAFRTALPADLKKSFEAFLLDLPKSHRKVYDDV
                     EKGAGVGYVPAAMELYQDIIDLRLAERSANRS"
     gene            4143..4814
                     /locus_tag="VGC82_11895"
     CDS             4143..4814
                     /locus_tag="VGC82_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEY0424013.1"
                     /translation="MANVVTIEAEVRARSGKGAARATRRAGKVPGVIYGGKQEPNLIA
                     LDPRVVIRELHRAGWQSRLYEITAGAEPIRALIRAVQFHPVSDAPEHVDFQRLVTGQR
                     IKVAVPVHFENDGISPGIKRGAVLNVVRHAVEVYCDADHIPEQFTADLGPLDFNDNVR
                     WHDLKGTENVKAVIDRDFVVATVVPPTKIEVVAEAAVAGAPTAAAAPAKGGSRAPGKA
                     PAKKG"
     gene            4862..>5184
                     /gene="pth"
                     /locus_tag="VGC82_11900"
     CDS             4862..>5184
                     /gene="pth"
                     /locus_tag="VGC82_11900"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY0424014.1"
                     /translation="MAANRHNIGFMAIDVIATRHGFSPWRRRFKGLTAEGAIAGQKIL
                     ALKPLTYMNNSGESVQEAASFYKLAHEAIVAFHDELDLTPGKMRVKKGGGAAGHNGLR
                     SMDKM"
BASE COUNT          927 a         1670 c         1699 g          888 t
ORIGIN      
        1 cgttccgcgc gatgctagaa gcaacggcgc gacaagccgt cgagacgctg acggaggcgg
       61 aagctgccga agaactcgag catctggcgc aggaaatcgc gcaccacgat gctgcgtatc
      121 atacccatga tgcgccggag atctccgacg ccgattacga tgcgctgcgc cgccggaatt
      181 cggcgatcga ggcgcgtttt ccggaactga tccgggctga ctctccgtcc agccgtgtcg
      241 gcggagcgcc tgagaccggc ttcgcgaaac ttcgccaccg cgttccgatg ctgtcgctcg
      301 acaacgcctt cgacgcggcc gaatttgccg aattttgcaa ccgagccaag cggttccttg
      361 gccgaaccga accgttggtg ttcgtcgcgg aaccgaaaat agacggactc tcgatcaacc
      421 tgacctatga gcacggtcgg ttcactcgcg gtgccacaag gggcgacggc acggaaggtg
      481 aagacgtcac cggcaacctt cgaacgataa agtcggtacc ggaccggctg cgcggcgagg
      541 cgccggcgct gatcgaaatc cgcggtgagg tcttcatgac caaggccgac ttcctcgcgc
      601 tgaaccaggc gcaggccgag gcggggggga aagtcttcgc caacccgcgc aacgccgccg
      661 ccggcagctt gcggcaactg gacgttcgga tcaccgccgg ccgccctctg tccctgtttg
      721 cctatgccca gggcgaaagc agcgaacctg tggctgccac ccactgggag tatctgcaac
      781 ggctgaagga ctggggtttc gaggtcaatc cgctgtcccg gcggcttgga agcgaggcgg
      841 acgccgaagc gttccagaat gagatcggcc tggcccggtc gggactgggc tacgacatcg
      901 acggcgtcgt ttacaagatc gacgaccttg ggttgcagcg ccgcctgggg ttcgtcggcc
      961 gtgcgccgcg ctgggcgacc gcctggaaat ttccggccga acaagccacg accgtactgc
     1021 gcgacatccg catccaggtt ggccgaaccg gcgcactgac cccggtcgcc gaactggagc
     1081 cggtcaacgt cggcggcgtc ctggtcgctc gggctacgct gcacaacgaa gatgaaatcg
     1141 cacggaaaga tatccgcatc ggcgacaccg tcgtgctgca acgcgccggg gacgtgatcc
     1201 cgcagatcgt gtccgtcgtg caagatcgcc gccggccgga tcaggctgca tttgtctatc
     1261 ccgaggtctg cccggcatgc ggaagcaatg ccgtgcggcc acccggggaa gtggttcgcc
     1321 gttgcaccgg cggcctgatc tgcccggcgc aacgggtcga acggctgatc cacttcgtct
     1381 cccgcccggc gttcgatatt gacggactgg gcgagaaaac catccaggag ttctacagcg
     1441 aaggctggct gcacggcccg gccgatctgt tcaagctgcc ggaacgggaa gccgaaatcg
     1501 ccaagcggga aggctggggc aaattgtcgg cgcggaacct gtcccgcgcg atcgccgcgc
     1561 gccggacggt gccgctggag cggttcatct tcgccctcgg gatccgccgc atcggctcga
     1621 ccaacgcgaa actgctggcc aggcattacg gcagctttgc caactggcgg gagcagatgc
     1681 tggcggcgac cacgatcggg tccgatgaac ggctggcact cggcagcatc agcggcatcg
     1741 gccccacgat cgccgaggaa ctgacggact tcttcggtga gccgcgcaac atcgccgcgc
     1801 tggacgaact tgccgccgag ttgaccattc aggacgccgc ccgagccgag gccgccgaca
     1861 gcccgatcgc cggcaaaacc gtggttttta ccggaacgct ggaaacactg tcgcggcctg
     1921 aagcgaaggc tcgggccgag gcgctgggtg cgaaggtaac ggattcagtt tcgaagaaga
     1981 ccgatatcgt ggtggtcggc gccgatgccg gttcgaaagc acgcaaggca acggagctcg
     2041 gcgtgcgaac gatgaccgag gcggagtggc aggaacttgt cggcgatcct gcgtaaccga
     2101 acctcggcca gcgatgaagc tagctcagcc agaaagcgcg gttcgctgtg ctggcgccgg
     2161 ggtgtgtccc ggtgttcgtc cgcgagatga tggccaaatc ccacgtcgtg gttggcttgg
     2221 ccacatggat cgccgcggca ccgctgctgc acattttccc gcttaacccg gcgtacctcg
     2281 gcttggcggt ggccgggtcg ctccttccgg acatcgacca tcccaaatcc tgggtcgggc
     2341 gccggacctg gccggtttcg acggcgctcg cgtcgatcct ggggcatcgc ggcgtgaccc
     2401 attcggcgct cgcggtcgtc ggcctggcgg cactgctgtt gcaggcaggg tatcggtggg
     2461 gcggcgtgtc cgcgctgatc gtcggctacc tgtcgcacct cgcggcggac atgctcacgc
     2521 cgcaaggtct caggctggca tggccgctgc gcgggacctg ggcgcttcca atatgccgca
     2581 ccggctcagt tatggagccg atcatcgtgg ccctcctggt gtgcgggatc ggctggtgga
     2641 ttttccggca cggcctcggt ggcgcacccg cgttgccgcg aaaaccatcg ggctgacggc
     2701 caccgactca aagttgtttc gcgctgactg aacaggattc tcctgtgctc gcaaagcaag
     2761 tcgtggctgg ccgcgccagg cacgggcatg acacgtcggc gccaggcggc acgccggctc
     2821 gcaccctttc cggccagcgt gaaagcgctg cagggagccc gcccgcaccc ggatcatcag
     2881 gcctgcaccc agatcagcct gcacccagat catcaggagc ggttggcact ccgttccgcc
     2941 agtcgcagat cgataatgtc ctggtagagt tccatcgccg ccggcacgta gccaacgcct
     3001 gcgccttttt cgacgtcgtc atagaccttg cgatgcgact tcggcagatc gagcaggaac
     3061 gcctcgaacg acttcttcag gtccgccggc agtgcggtgc gaaacgccca gggaccgttg
     3121 gggattttcc ccgactgcca gatgatgtcg atgtccgaca tcttcagcat cttcctgtcc
     3181 accatcgagc gcaggttgcc ccgcgtgtag ccctccgact tctcgccctg gcccgacgtc
     3241 cacgttacgg cggcgtcgta ctgccggttc agtaccgcga ccaccccttg ttcgtggccg
     3301 cccgagaacc ctgtcttggt aaaatacccg ccgtccttca ggtcgatccc cttggtcttc
     3361 agcgtggcgc ttgggatcag gtaaccggac gtggaattgg gatcggccca ggccagcgag
     3421 cggcccttca tgtcagccag cgacttgatc ccggaatcgg cccgcacgac catgaccgag
     3481 tagtagtacg tcgagccatc cgcttcttgc ggcaccacca cgggctcgat gcatttgcag
     3541 tccagccagg cgccggcaaa gccggacgca ccgaactcgg cggcctccag ttggccggcg
     3601 gcgatccctt gcatgacacc ggcataatcg gctgccggaa acagcttcac cggcatcttc
     3661 aggtgtgctt cgagcagctt ctgaaactcg tcatagcgcg ttaggcgatc ctgcgtgttt
     3721 tcgccgccca ggatgccgat gcggaattcc ttcacggtct gggcacgggc gcgcctgccg
     3781 ctgcccaggc cgaatcctaa tgcaacggcg ccggtagcga tggcctgtct gcgtagcatg
     3841 ctgctgtttc cccttgtggt cggcccggca gtagcgtgag gtcgatgaca agcggatggc
     3901 agttgggtga aaagacgatg tcagaactca gcccgacggg taacccttgg aacggcatgt
     3961 gacgcagcgc caggaggccg gcggtaccgg catctgatgg gatttttgat gccgaaaagt
     4021 ggccggcgcg gagcgacaag cggacaaatg cgccataacc cttgcccccg gccccgccct
     4081 gggctacacg cccggcgcgt cggcacccct ggaggccggc gtttgtcatt caggagcgaa
     4141 ctatggccaa cgttgttaca atcgaggccg aggtacgcgc gcgaagcggt aagggggcgg
     4201 cgcgggctac tcggcgggca ggcaaggtgc ctggtgttat ttatggcggc aagcaggaac
     4261 ccaacctgat cgcgctcgac ccaagggtgg tgatccgcga gctgcatcgg gccggctggc
     4321 agtcccgttt gtatgaaatc accgccggag cggagccaat ccgggcgctg atccgcgccg
     4381 tacagtttca tcccgtgtcg gatgcgccgg agcatgtcga tttccagcgc ctggtcaccg
     4441 gccagcggat caaggtggcg gttccggtcc atttcgagaa tgacggaatc agccccggga
     4501 tcaagcgggg tgcggtgctg aacgtcgtgc gccacgcggt ggaagtgtat tgcgacgccg
     4561 accacattcc ggagcagttc accgccgatc tcgggccgct ggatttcaat gacaacgtcc
     4621 gctggcacga cctgaagggc accgagaacg tcaaggcggt gatcgaccgc gacttcgtgg
     4681 tggccaccgt ggtgccgccg acgaagatcg aggtggttgc cgaggcagcc gtcgccggcg
     4741 ctcccaccgc tgctgccgct ccggcaaagg gcggctcccg cgcacctggc aaggcgcctg
     4801 ccaagaaggg ctgactgccg tgacgcagct ctgggtcggc ctcggcaatc cagagcctgg
     4861 gatggcggcc aaccgccata acatcgggtt catggcaatc gacgtgatcg cgacccgcca
     4921 cggtttctcg ccgtggcggc gccgcttcaa gggcctgacg gccgagggcg ccatcgccgg
     4981 acagaagatc ctggcgctga agccactgac ctacatgaac aactcgggcg agtccgttca
     5041 ggaagccgct tccttctaca agctggcgca tgaggccatc gtcgcgttcc acgacgaact
     5101 cgacctgaca cccggcaaaa tgcgggtcaa gaagggcggc ggtgccgccg ggcataacgg
     5161 attgcgcagc atggacaaga tgtt
//
LOCUS       DASWQN010000701         3196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2442688, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000701 DASWQN010000000
VERSION     DASWQN010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2442688"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VGC82_11905"
     CDS             complement(<1..546)
                     /locus_tag="VGC82_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HEY0424015.1"
                     /translation="MIPYIPAIVLLHGACAVIYAALAALILARPPPSRTGVWLAFACA
                     VTAVWAASFALAGQAPVGRLAVWLELARSAAWYGFILHLYRRSIGANGQVATAFRTMG
                     LLALLLLAGTPLVEWLSGPATPSFQSLGTAAKLGLAVCNVLLLENLYLNTPSDSRWNI
                     NLLCVALGGVFLYDLLIYADGV"
     gene            complement(590..1930)
                     /locus_tag="VGC82_11910"
     CDS             complement(590..1930)
                     /locus_tag="VGC82_11910"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY0424016.1"
                     /translation="MTPSAASHHPRREMAVLGLLETLLSFAAIYAMIRVAAASATFPS
                     TFGVLPRNDFGLAVILTIVAGVAGLAIGLYRTDTRLSRQRLLITTSLAACIAFGILLV
                     VSGAGVRAGQALSIALLLGAWLATLILVRLTYGPATERMFRARRILLVGEPRRIGAVN
                     ARLRSRRGQRFDPVLNHAADMSWALLRQQRIWGVVLTSEPEDPAIVPLLDCKLRGVKV
                     LSGAAFNEKYLGRIDLDTLSANDLLFDQGFAVSRFDDAVKRLNDVVVGLAMLVLAVPL
                     MALTALAIKIDSRGPVLYRQQRIGRYNEPFTLFKFRSMRVDAEAGGPRWAQKHDSRIT
                     RIGGFIRMTRIDELPQLANVIMGEMSLVGPRPERPHFVEQLARVIPFYRQRAYVKPGL
                     SGSAQVNFPYGASVEDAREKLAYDLFYVKNRSVFLDTIILLSTVRVVLFREGAR"
     gene            2682..>3196
                     /locus_tag="VGC82_11915"
     CDS             2682..>3196
                     /locus_tag="VGC82_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HEY0424017.1"
                     /translation="MIEKSGVKAPDDYIIGSGDQLSIFVYRNPDLSEGAVAVRPDGRI
                     STPLIEDIVASGKTPTKLAREIEGRLTKYVQEPNVTVIVRGFIGPPDRQVRVIGEATD
                     PIAIPYRDHMTLLDVMIATKGLTKYAAGNRAVLVRVDPDGKQQSIKVRLNDLIKDGDI
                     GQNIEMNAGDTL"
BASE COUNT          681 a          951 c          987 g          577 t
ORIGIN      
        1 cacgccgtcg gcgtagatca gcaggtcgta caggaagacg ccgcccagcg cgacgcacag
       61 gaggttgatg ttccagcgtg agtccgacgg cgtgttgaga tacaggtttt ccagcaacag
      121 cacgttgcag accgccagcc ccagtttagc cgcggtgccc agcgactgga aactgggtgt
      181 ggcggggccc gacagccact ccaccagagg agtgccggcc agcaacagga gggccagcag
      241 ccccatggtc cggaaggcgg tcgcgacctg gccgttggcg ccgatcgacc gccggtaaag
      301 gtgcagaata aagccatacc acgccgccga acgggcgagt tccagccaaa cagccaagcg
      361 gccgaccggc gcttgcccgg caagggcgaa cgacgcagcc cagaccgccg ttactgcgca
      421 ggcgaaggcg agccagacgc ccgtccggct gggcggcggg cgcgccagga tcagggcggc
      481 gagcgcggca tagatcaccg cgcaagcgcc gtgcagcaga acgatcgccg gaatgtacgg
      541 gatcatgagc gcagctatcc ggcctgtcgc atcgtctgca ttccggcgtt cagcgtgcac
      601 cttctcggaa cagcaccacc cggacggtcg aaagcagaat gatcgtgtcg aggaaaaccg
      661 accggttctt aacatagaac aaatcgtagg cgagcttctc gcgagcatcc tcgactgagg
      721 cgccgtaggg gaagttgacc tgcgccgaac cgctcaaccc gggtttgacg taggcgcgtt
      781 ggcggtagaa tgggatgacg cgagccagtt gttcgacgaa atgaggccgc tccggccttg
      841 gtcccaccag gctcatttct cccataatga cgttagctaa ttgcggcagt tcatcgatcc
      901 gggtcatccg gataaacccg ccgatgcggg tgatccgcga atcatgcttc tgggcccagc
      961 gcgggccgcc agcctcggcg tccaccctca tgctgcggaa cttgaacaag gtaaatggct
     1021 cgttgtatcg tccgatgcgc tgctggcgat acaggacggg gccccgactg tcgatcttga
     1081 tcgccagcgc ggtcagcgcc atcaacggca ccgccaggac aagcatggcc agtccaacga
     1141 ccacgtcgtt caatcgcttt accgcatcgt caaaccgact caccgcaaac ccctggtcaa
     1201 aaagcaagtc gttcgcgctc agggtgtcca gatcgatccg gcccaggtat ttttcgttga
     1261 aagcggcgcc gctcaggacc ttcacccccc gcagcttgca atccaacaac gggacgatgg
     1321 ccgggtcttc gggttccgag gtcagcacga ccccccatat ccgttgctgg cgaagcagcg
     1381 cccaggacat gtctgctgca tgattcagca ccggatcaaa ccgttggccg cgccgggacc
     1441 gcagtcgggc gtttacggca ccgatgcgac gcggctcgcc gacgagcaga attcgtcgtg
     1501 cccggaacat ccgttcggtg gcgggaccgt atgtcaggcg gacgaggatc agggtggcca
     1561 accaggcgcc cagaagtaat gcgatcgaca aagcctggcc ggcacggacg ccggcgccgc
     1621 tgacgaccag caagatgccg aaagcgatgc aggcggcgag gctggtcgtg atcaggagcc
     1681 gttggcggct gaggcgcgta tccgtccggt acaggccgat ggcaagcccc gccacaccgg
     1741 cgacaatggt caggatgacc gccaggccga agtcattgcg gggcagtacg ccaaatgtgc
     1801 tgggaaacgt agccgatgcg gcggcgacgc ggatcatggc gtagatcgcc gcgaaagaca
     1861 gcagggtctc cagcagacca aggaccgcca tttcgcgccg cggatggtgc gaagccgcgg
     1921 atggcgtcaa ggctttcgtt tccatccgtc cagccccggg ttgaatagac gatagttccg
     1981 gggcaccccg gctcccgatc ggcggaacga atgtgcgtgg ggcatttttg ccgtgatgtt
     2041 tgggagaatc tattatttaa acaggtagca gccgcggaac gacaggcgag cgaacgttgc
     2101 agattggtgc atagcaggtc aatgcttttt tcaaattcga tgtttgtttg aaaaaccgag
     2161 ctatggtgcg aaatatcttt tatgtttaga aagttgtcat gttagtagat gagctcctgc
     2221 gatttgaggt gcaacttttg cctgaccgca acatagacta aacttgtctt ttgcaacacg
     2281 ttgcgtcggc gcgttgaatc gacgcgggcg tcatgttata tgaaacctta tatgttgcag
     2341 agcttgtcag gctaatctcg tgcgggaggc atagacttca ttaccacagc aggaggtttg
     2401 agttctttcc ccgatcaatt ggccccacta tcaacggcaa catcggccac tcaccctttc
     2461 cttccgctca tccgatcacc agccccggcg gtcggcggcg tttgatccat tcagaggttg
     2521 acgtgatccc atcagcgttt cagagccgat tagccgtagg cccctcgggc gggagatgtg
     2581 gttccgccgt caaagctctt cgcttgaatt gggcattcag ccttatgttc gtactaggcg
     2641 gctgcggcag cagccttttc aacggccgcg cggatccgcc ggtgatcgag aagtcaggcg
     2701 taaaggcacc cgacgactac atcatcggat cgggcgacca gcttagtata ttcgtctatc
     2761 gcaatcccga cctcagcgaa ggcgctgttg ccgtccgtcc ggacggacgt atctccacgc
     2821 cgctgatcga ggacatcgtg gcatccggaa aaacaccgac caagctggcg cgcgagatcg
     2881 aggggcgcct gaccaagtat gtccaggaac caaacgtcac ggtgatcgtg cgaggcttca
     2941 tcggtccgcc ggatcggcag gtgcgggtga tcggagaggc aacggatccg attgccattc
     3001 cgtataggga ccacatgact ttgctcgacg tcatgatcgc caccaagggg ctgacgaagt
     3061 acgccgccgg caaccgcgcc gtccttgtgc gggtcgatcc ggacggcaag cagcagtcga
     3121 tcaaggtaag gctgaacgat ctgatcaagg acggcgacat cggccagaac atcgaaatga
     3181 atgcaggcga caccct
//
LOCUS       DASWQN010000702         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_860015, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000702 DASWQN010000000
VERSION     DASWQN010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860015"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VGC82_11920"
     CDS             <1..649
                     /locus_tag="VGC82_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0424018.1"
                     /translation="TRLLQGINREYSGFIKIDGADLREINLRHLRSSFGVVLQENFLF
                     RGSIRDNILAGRPGLTLEDAIRAARLAGAEEFIERMPNGYETYIEEGSPNLSGGQRQR
                     LAIARALITDPRILILDEATSALDPESEALVNANLLRIGRGRTMLIVSHRLSSLVNCD
                     QILVLERGKVMDIGPHSVLLERCAIYRTLWNQQHRHMQNEGSGHAALAPTLVQGD"
     gene            612..2021
                     /locus_tag="VGC82_11925"
     CDS             612..2021
                     /locus_tag="VGC82_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEY0424019.1"
                     /translation="MQLLPRPSSRAISTTAEDRHDPTLPVILEYQSPSTAIINMPMPR
                     IASGFTWTFASMIAVLCTLAGVIEVDRVVTATGVVVARSPTIVVQPLETAIVRSIEVH
                     PGDVVHAGQILARLDPTFAAADMGALVAQVSSLQAQVSRMQAEMENRPFNYAGLDPNM
                     ALQAAIYGQRQAEFNFRLENYKQKGDSLLAAIARARGDTTNYADRLAYAKTLEQMRKE
                     LEHLNVGSKINTLSAMDTRVQMQAGLDTAQQQLLGSQRDLDALLAERNGYVQTWHTDI
                     ADKLTDALGKLSDARESLNKAQLRRQLVELRAEQDGTVMTISKVSVGSVLNPGQQFIT
                     LVPTDAPLEVEANIAGSDDGHVSVGDPVDVKFDTFQYSRYGLAHGLVRIVSPDSFSAQ
                     DESRNPSGAVPLPNSANGVYYRSRITLDRIELHNTPANFHLTPGMPVSADIRVGKQTI
                     LRYMLGKTIPLATEGLREP"
     gene            2055..>3473
                     /locus_tag="VGC82_11930"
     CDS             2055..>3473
                     /locus_tag="VGC82_11930"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEY0424020.1"
                     /translation="MAFFDRLISGFSSAASLRRGVRMQEQGNARAAFVLLSRAGRANI
                     PEAEFRVGRCYLEGAGVPPSRADGVRWVERAAAKGYVEAQALLATLCLHGMAPGFADA
                     GSGAGGSLFGGSNTAEPDYVSAAKWARRAAEAGSVEGQAVLGYILTSGPENLRNAEEG
                     DRWYEKSAAGGCPQGQLGYALVLARDTTKPGAQEELLRHLSQAAEKGLATALYLYGMV
                     LERGVGAAQDRSAAVACYKQAAEKGHRAAQARWGFALMEGNGVEANPVEGESWLRRAA
                     LAGDPEAAALVGDIYAKGGSLPPNYAEAAMWFRRAADANHRGATRALAMLYLTGAGVP
                     RDQEESARLFSIAAQAGDATAHVDLGNLLLKGHGDEQDQIRTRSWFEQAAASGDLIAA
                     FNFGVCLAEGVGIERDDRKAAEWLRRAADGVVNAQYWYGRMLVEGRGVEANPEEGRVW
                     ITKAADVGMIEAEVMLADMMLSG"
BASE COUNT          636 a         1103 c         1137 g          597 t
ORIGIN      
        1 cacccgattg ctgcagggca tcaatcgcga atactcgggc ttcatcaaaa tcgacggtgc
       61 cgacctgcgg gaaatcaacc tgcgtcacct acgctccagc ttcggcgtcg tgctgcagga
      121 gaacttcctg ttccgcggct cgatccgcga caacatcctg gccggccgtc ccgggctgac
      181 gctggaagat gcgatccgag cggcgcgcct ggccggggcc gaggaattca tcgagcggat
      241 gccgaacggg tacgagacct acatcgaaga aggctcgccc aacctgtcgg gcggccagcg
      301 gcaacgcctc gccatcgccc gtgccttgat caccgatccg cgcatcctga ttttggatga
      361 ggccaccagt gcgctggatc cggaatccga ggcgctggtg aacgccaacc tgctgcggat
      421 cggccgtggc cggaccatgc ttatcgtgtc ccaccgtctg tcatccctgg tcaactgcga
      481 ccagatcctg gtcctcgaac gaggcaaggt catggatatc ggaccgcatt cagtgctgct
      541 cgagcggtgt gcgatctacc gcacgctctg gaaccagcaa caccgtcaca tgcagaatga
      601 aggctcaggt catgcagctc ttgccccgac cctcgtccag ggcgattagt accaccgcgg
      661 aggaccggca cgatccgact ctgccggtca tcctggagta tcagtctccc tccaccgcta
      721 tcatcaacat gccgatgccg cggatcgcca gcggcttcac ctggacgttc gccagcatga
      781 tcgccgtgct atgcacgctg gccggcgtca tcgaggtgga ccgcgtggtg accgcgaccg
      841 gtgtggtggt ggcacgctcg cctacgatcg tggtccaacc gttggaaacc gccatcgtcc
      901 gctcgatcga ggtgcatccc ggcgacgtgg tgcatgccgg ccagatactg gcccggctcg
      961 atccgacttt cgcggcagcc gatatgggcg cgcttgtggc gcaggtgtcc tcgttgcagg
     1021 cacaggtttc gcgcatgcag gcggaaatgg agaaccggcc gttcaactat gccgggctcg
     1081 accccaacat ggctctgcag gccgccatct acggccagcg gcaggccgag ttcaacttcc
     1141 ggctggaaaa ctacaagcag aagggggaca gccttctggc ggcaatcgcc cgtgcgcgtg
     1201 gggataccac gaactacgcg gaccggctgg cgtacgccaa gaccctcgag cagatgcgca
     1261 aagaacttga gcacctgaac gtcggctcca agatcaacac cctttcggcg atggacacgc
     1321 gggtgcagat gcaggctggc ctcgacaccg cccagcagca gttgctcggc tcgcaacgcg
     1381 accttgatgc gttgctcgcc gaacgcaacg gctacgtgca gacctggcat actgatatcg
     1441 ccgataagct gaccgacgcc ctgggcaagc tttcggatgc ccgggagtcg ctgaacaagg
     1501 cgcagttgcg ccggcaattg gtcgagctgc gcgccgaaca ggacgggacc gtgatgacga
     1561 tcagcaaggt gtcggtgggt tcggtgctga acccggggca acagttcatc acgctggtgc
     1621 cgaccgacgc gccgctggaa gtcgaagcca atatcgccgg cagtgacgac ggccacgttt
     1681 ccgtgggcga tccggtggac gtcaaattcg acaccttcca gtattcccga tacggcttgg
     1741 cccacggtct ggtgcggatc gtcagtccgg acagcttcag cgcccaggac gaatcccgca
     1801 atccctccgg cgcggtccca ctcccgaatt cggccaacgg ggtgtattat cggtccagga
     1861 taacgctcga tcggatcgaa ctgcacaaca ctccggccaa cttccacctc acgcccggca
     1921 tgccggtcag cgcggacatc cgcgtaggca agcagaccat cttgcgctac atgctcggaa
     1981 aaacgatccc cttggctacc gaagggctgc gggagccgta aggccccgca ttcccggcag
     2041 gacgcaacca gcacatggcc ttttttgaca ggttgatcag tggattttcc tcggccgctt
     2101 cgttgcgccg gggcgtccgg atgcaggaac agggcaacgc gagagccgca ttcgtgctgc
     2161 tttcgcgggc cggccgagcc aatattccgg aagccgaatt ccgggttggg cgctgctacc
     2221 tggaaggcgc cggcgttccg cccagtcgtg cggacggcgt ccggtgggtc gaacgggccg
     2281 cggccaaggg ctatgtggag gcacaagcgt tgctcgccac gctatgcctg cacggtatgg
     2341 ccccgggctt cgccgacgcg ggttccgggg cagggggcag cctgttcgga ggctccaaca
     2401 ccgccgaacc ggattacgtc agcgccgcca aatgggcgag gcgggcggcc gaagcgggat
     2461 ccgtcgaagg gcaggcggtg ctgggctaca tcctgacatc aggtccggag aacctgcgca
     2521 acgccgagga aggcgatcgc tggtatgaaa aatccgctgc ggggggatgt cctcagggcc
     2581 agctcggcta cgcgctcgtg ttggcgcgcg ataccaccaa acccggcgcg caggaagaac
     2641 tgcttcggca tctgagccag gcggcggaaa agggcctggc aacggcgctt tacctttacg
     2701 gcatggttct tgagcgtggc gtgggggctg ctcaggatcg aagcgccgcc gtggcttgct
     2761 ataaacaagc tgccgagaaa ggtcatcgcg ccgctcaggc ccgctggggt ttcgctttga
     2821 tggagggcaa cggcgtcgaa gccaacccgg tggaaggcga atcgtggttg cggcgtgccg
     2881 cgttggcggg cgatccggaa gctgccgcat tggtgggcga catctatgcc aagggcggct
     2941 cgttgccgcc aaactatgcc gaggcggcga tgtggttccg ccgtgccgcc gacgccaatc
     3001 accgtggtgc cacccgcgcc ctggccatgc tgtatctgac gggcgcaggt gtcccgcgtg
     3061 accaggagga atccgcccgg ctgttcagca tcgcggccca ggccggcgat gccaccgccc
     3121 atgtcgatct tggcaatctg ctgctgaaag ggcacggcga tgaacaggat cagatccgca
     3181 cccggtcctg gttcgagcag gctgcggcct cgggcgatct gatcgctgcg ttcaattttg
     3241 gcgtctgcct cgcggaaggg gtcggcatcg agcgcgacga ccgtaaggcg gctgagtggc
     3301 ttcgccgtgc cgccgacggc gtcgtcaatg cccagtattg gtatggccgc atgctggtcg
     3361 aaggccgcgg cgtcgaagcc aaccccgagg aaggccgcgt ctggattacc aaggccgccg
     3421 atgtcggcat gatcgaggcc gaggtaatgc tggcggacat gatgctgtcc gga
//
LOCUS       DASWQN010000703         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_68304, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000703 DASWQN010000000
VERSION     DASWQN010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68304"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VGC82_11935"
     CDS             <1..1080
                     /locus_tag="VGC82_11935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY0424021.1"
                     /translation="VLLGDCVPLMAMLPPASVHCVFADPPYNLQLRGELRRPDDSLVD
                     GVDDDWDRFADFAAYDAFTREWLGECRRLLRKDGTIWVIGAYHNIFRIGAILQDLGFW
                     ILNDVIWRKSNPMPNFRGRRFTNAHETLIWAARGRDSRYRFNYQAMKSLNDDIQMRSD
                     WFIPLCTGPERMRNEHGLKLHPTQKPEALLHRVLLASTNQDDIILDPFTGTGTTPAVA
                     KRLRRHFIGIERHPAYAEAAIGRVRREKPISVEGATSQPTKREATRIPFGSLVEQGLI
                     AAGTRVFDRQRRVSAVVGPDGSLSTGDLRGSIHKIGALLQNAPSCNGWTFWHFERDGS
                     LHPLDMLRSEVAGQTGAPAPDPLAL"
     gene            1077..1667
                     /locus_tag="VGC82_11940"
     CDS             1077..1667
                     /locus_tag="VGC82_11940"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEY0424022.1"
                     /translation="MTDLNAIKLATRDLHTRAERSGIIADILAGRASRLGVALLLRNL
                     LPVYQLLDASALGHPALARSDVIKADLLVLSPGVDLPLLTQGQAYADWVTRASDGDTS
                     GLLGHIYVRYLGDLNGGRIMQRRLAACLGEVGGALAFGHYPVLDNEDNFRRDYREELD
                     RAVRVAKFETVKREALAAFELNIVLSEAVKSHVESA"
BASE COUNT          315 a          527 c          625 g          357 t
ORIGIN      
        1 gtcttgctcg gggactgcgt gccgctcatg gccatgctgc cacccgcgtc ggtccactgc
       61 gtttttgccg acccgccgta taacctgcag ctccgcggcg agctgcgccg gcccgatgac
      121 agcctggtcg acggcgtgga cgacgactgg gaccggtttg ccgacttcgc cgcctacgac
      181 gccttcaccc gcgagtggct gggggagtgc cggcggctgc tgcgcaagga cggcacgatc
      241 tgggtgatcg gcgcctacca caacattttc cgcatcggcg ccatcctgca ggatctgggg
      301 ttctggatcc tcaacgacgt gatctggcgg aaatccaacc caatgccgaa tttccgcggg
      361 cggcgcttta ccaacgcgca cgagacgctg atctgggccg ctcgcgggcg ggactcgcgc
      421 taccggttca attaccaggc gatgaagtcg ctgaacgacg atattcagat gcggagcgac
      481 tggttcattc ccttgtgcac cgggcccgag cggatgcgca acgagcatgg gttgaagctg
      541 catcccacgc agaagcccga ggcgctgctg caccgcgtac tgctggccag cacgaatcag
      601 gatgatatta tcctggaccc gttcaccggc acgggcacga cgccggcggt ggcgaaacgg
      661 ctgcgaaggc attttatcgg tatcgagcgg catccggcct acgccgaagc ggccattgga
      721 agggtgcggc gggagaagcc gatctctgtc gaaggcgcga cgtcgcagcc gacgaaacgg
      781 gaagccaccc gcataccgtt cggcagcttg gtggagcagg gtttgatcgc ggcgggaacc
      841 agggtgttcg atcgccagcg gcgtgtcagc gcggtggtag ggccggacgg atcgctgagc
      901 accggggatc tgcgcgggtc gatccacaaa attggagcgt tgttgcagaa cgcgcctagc
      961 tgcaatggct ggacgttttg gcacttcgag cgcgacgggt cgctgcatcc gttggatatg
     1021 ctgcgttcgg aagtggctgg ccaaaccgga gcgccggcac cggaccctct ggcattatga
     1081 ccgacttgaa tgcgatcaag ctggcaaccc gtgacttgca cacgcgggcg gagcgatccg
     1141 gtatcatcgc cgatatcctg gcagggcggg caagccgact gggtgtggcg ctgctgttac
     1201 ggaatttgtt gccggtctat cagttgctag atgcatctgc gttggggcac ccggcgctgg
     1261 cccggtcgga cgtcatcaag gctgatttgc tcgtgctgtc tcccggtgtt gacttgcccc
     1321 tgctaacgca ggggcaagcc tatgccgatt gggtgacgcg ggcctcggac ggtgacacat
     1381 cggggttgct tgggcatatc tacgtgcggt acctgggcga tctgaacggc ggccggatca
     1441 tgcagcggcg gttggccgcg tgcctgggcg aggtcggcgg ggcgttggcc ttcggccatt
     1501 atccggtatt ggacaacgag gacaacttcc ggcgcgacta tcgcgaggaa ctggatcgcg
     1561 ctgtgcgtgt cgcgaagttc gagacggtga agcgcgaagc cttggctgcg ttcgagttga
     1621 atatcgtgtt atcggaggcg gtgaaaagcc atgtggagtc cgcctgaggg caaagctgtt
     1681 tgtggtcact cctccagaag gcgatacatc tttccgtcaa actgaacgag ccgcatgctt
     1741 ttcatcggcc ggaagtcggt ggggctgctg ctgattttga tccccggaag caccatcggc
     1801 agtgtcgtcg tttccggcag gctg
//
LOCUS       DASWQN010000704         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_860295, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000704 DASWQN010000000
VERSION     DASWQN010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860295"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VGC82_11945"
     CDS             <1..699
                     /locus_tag="VGC82_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424023.1"
                     /translation="LIGMLTWRGLLMSYVALMLLVNLPMVWLLVPPAPPRPKPGAAEA
                     GVAKSPAERRAVVLLGGFFALRWFITSAIAVHVLPLLRGVGLTAAEAVGIAAMIGPGQ
                     VAGRIIEYAVGPRINLLVKARIGALLFPAGAMLLLFGGPFAVAGFAVLYGMSNGIMTV
                     NRGTLPLAMFGPAGYAQMLGWLAVPVLLAQATAPTLTAPLVAALPAADVLLISGAAAA
                     VAVMFLLPLRLPAS"
     gene            complement(802..1716)
                     /locus_tag="VGC82_11950"
     CDS             complement(802..1716)
                     /locus_tag="VGC82_11950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0424024.1"
                     /translation="MVPADEDFDGTWPFAPCFTDAAGFHQHYIDEGTGRPILLLHGQP
                     TWGYIWRRFIPALAKTHRAIVPDHMGFGKSETPPDRDYTLRTHVENLTALIDALDLHD
                     ITLVMQDWGGPIGVGYAVRHPERIHSLVLMNTVVGYGTAGRRDLPNPVETPWFRWIRD
                     GLPTGRTEAVLSQLGSTILSVMQIVGLERLDRVDPTFIRAYGAPFATPADCKGAVEFP
                     LDLALGRIRDFVREGASGVPALRDKPALLIEGMLDRAIPPALALADFAGIWPKAPIIQ
                     VPGAGHYIQEDAPEIVVPALQTFLGMVG"
     gene            complement(1826..2194)
                     /locus_tag="VGC82_11955"
     CDS             complement(1826..2194)
                     /locus_tag="VGC82_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0424025.1"
                     /translation="MIDAQDLAQRYIASWNESDPVVRRQLVDRLWSQDARYADPMMKA
                     DGQDGIAELIGGVHRQFPGYCFALTGQADGHGLFVRFSWSLAPAGGRAVAFGTDFASV
                     APDGRLEQVTGFLDQVPGTA"
     gene            complement(2395..3114)
                     /locus_tag="VGC82_11960"
     CDS             complement(2395..3114)
                     /locus_tag="VGC82_11960"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0424026.1"
                     /translation="MHHETLTYQADGLTMRSQLFFEPSSGRRAGVLVFPEAFGLGEHA
                     ISRAERLAALGYVALACDLHGEGRVVDDLQEAIGLLQPLLSNPSRTRARAAGGLKALT
                     SRPEADPARVASIGYCFGGTMSLELARSGADLKAVVGFHSGLGTSAPKSDAKAIKARV
                     LVCIGADDPMIPPEQRAAFETEMREAGVDWQMHLYGNTVHSFTNPEAAKRNMPDAIRY
                     NADADERSWAALQKLFAETLT"
     gene            complement(3356..4150)
                     /locus_tag="VGC82_11965"
     CDS             complement(3356..4150)
                     /locus_tag="VGC82_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEY0424027.1"
                     /translation="MNDVIAALVRRHDPDRFLTALFAAPDRRDALLTLYAFNHELARA
                     REVASQAPLALIRLQWWREVVEGDRKRHEVATPLVDAISAGTLQPADLLPLIEARETE
                     VYGDFETTEEWRAWVLAGAGGLAVAAAGALGAPEAEAVRPFGAAYGVAGLLRASGVLA
                     SQGSCLLPHDLLRRHGVSPEAFIHDPGSEPARAALLDVVREGQALLAEARRIAVPRMA
                     VAAVLPAVLARRDLARWPDVAIPRRMGDRLAVTVAGMTGAGLTSAG"
BASE COUNT          757 a         1499 c         1445 g          752 t
ORIGIN      
        1 ttgatcggga tgctgacatg gcgcggcctg ctgatgagct acgtggcgtt gatgctgctg
       61 gtcaacctgc cgatggtttg gctgctggtg ccaccggccc cgccccgccc aaagccgggt
      121 gccgcggagg ccggcgtggc aaaatcgcct gcggaacggc gtgcggtggt gctgcttggt
      181 ggattctttg cgttgcgctg gttcatcacc tcggccatcg cggtgcatgt cctgccgttg
      241 ctgcggggcg tcggactgac cgcggccgag gccgtcggca tcgccgcgat gatcggtccc
      301 ggtcaggtcg ccggacggat catcgagtat gccgtcgggc cgcgcatcaa cctgctggtc
      361 aaggctcgga ttggcgcatt gctgttccca gcgggcgcga tgttgctgct gttcggcggc
      421 ccattcgccg tggccggttt cgccgtgctc tacggcatga gcaacgggat catgacggtc
      481 aaccgcggca ctttgccgct cgcgatgttc ggtcccgcgg gatatgccca gatgctgggc
      541 tggctggcgg tgccggtgct gctggcgcag gcgaccgccc cgacgttgac cgcgccgctg
      601 gtcgccgcat tgccggcggc cgatgtcttg ttgatcagcg gcgcggccgc ggccgtcgcg
      661 gtgatgttcc tgctgccgct gcggttgccg gcttcctaac cgacggtcac gccggtgcat
      721 cgttcagagc gtgcggatcg tgcgattcaa atagaccaat cgcagcctgc attatcaggc
      781 cgccaatggc ctgaccgccc gctaccccac catcccgagg aaggtctgca gcgccggcac
      841 cacgatctcc ggcgcatcct cctggatata atgtccagct cccggcacct ggatgatcgg
      901 cgccttgggc catatccccg cgaagtcggc cagtgccaga gcgggcggga tggcccgatc
      961 cagcattccc tcgatcagca gcgcgggctt gtcgcgcagc gccggcacgc ccgaggcgcc
     1021 ctcacgcacg aagtcgcgta tccgccccaa cgccaggtcc agcggaaatt caacggcccc
     1081 cttgcagtcc gccggggtag cgaacggtgc gccataagct cggatgaacg tcggatcgac
     1141 ccggtccagg cgctccagcc cgacgatctg catcaccgac aggatggtgg aaccgagttg
     1201 cgacaatacc gcttccgttc gcccggtcgg caggccgtcg cggatccagc gaaaccacgg
     1261 agtttccacc gggttgggca gatcgcgccg ccccgctgtg ccgtagccga cgacagtatt
     1321 catcaacacc aggctgtgga tgcgttcggg atgccgcacc gcatagccaa cgccgatcgg
     1381 gccgccccag tcctgcatca cgagggtgat gtcatgcaga tccaacgcgt cgatcagtgc
     1441 cgtcaggttc tcgacatggg tgcggagcgt gtagtcccgg tcgggcggcg tttcgctttt
     1501 gccgaagccc atatggtcgg ggacgatggc ccggtgggtc ttggccaggg cggggatgaa
     1561 gcgtcgccag atgtagcccc aggtgggctg tccgtgcagc agcaggatcg gccggccagt
     1621 gccctcgtcg atgtagtgct ggtggaagcc ggcggcgtcg gtgaagcacg gtgcgaacgg
     1681 ccacgtgccg tcgaaatcct cgtccgccgg aaccatggtg actgcctccc gtgttcaggc
     1741 gcccgtattc aagggccaat gttaaacggc cagtgttcag ggaagtgtgc ccgcccccgt
     1801 gcatcggggg aaggcacaac gatgctcagg cagtgccggg aacctgatcg aggaagccgg
     1861 ttacctgctc cagccgtccg tccggcgcca ccgacgcgaa gtcggtgccg aacgcgacgg
     1921 cccgaccacc ggccggcgcc aggctccagg aaaatcgaac aaacagaccg tggccgtcgg
     1981 cctggccggt caaggcgaag cagtatccgg gaaactgacg atgcacaccg ccgatcaact
     2041 cggcgatgcc gtcttgcccg tctgccttca tcatcgggtc ggcgtagcgc gcatcctggc
     2101 tccagagccg gtccaccagt tgccgccgaa ccacgggatc ggactcgttc caggacgcaa
     2161 tgtagcgctg cgcaagatct tgagcgtcga tcatgggttt tttccttgtg cctcaccggc
     2221 tggattgccg gcgagggcag aggatgcgga caggcgggaa tgaaagcgat tacgtcagag
     2281 gtaaaaccgc ttgccgatct accgcttgcc gatctggtga attcgcatcg cagcggagcc
     2341 tgatcgacca gggtttccaa ctcatggtcg aacgaccgca aggtccggcg gcggctacgt
     2401 caacgtttcc gcgaacagct tctgcagcgc agcccaggag cgctcgtcgg cgtcggcgtt
     2461 atatcggatc gcgtccggca tgttgcgctt tgccgcttcc gggttcgtga agctatgcac
     2521 ggtgttgccg taaaggtgca tctgccagtc cacaccggct tcacgcatct cggtttcgaa
     2581 cgccgcacgc tgttcgggtg gaatcatcgg gtcgtccgcg ccaatgcaaa ccaggacgcg
     2641 ggccttgatg gccttggcat cggatttcgg cgcgctggtt cccaacccgc tgtggaagcc
     2701 gacgaccgcc ttcaggtcgg cgccggagcg ggcaagttcg agcgacatgg tgccgccaaa
     2761 gcagtagccg atcgatgcca ctcgggcagg atctgcttcg gggcggctgg tgagggcctt
     2821 cagccctccg gccgcgcggg cgcgtgtgcg gcttggattg gataacaaag gctgcagtag
     2881 gccaatcgct tcctgaaggt cgtcaactac gcgcccctca ccgtgaagat cgcatgccag
     2941 ggcgacatag cccaatgcag ccagtcgttc ggcacgcgaa atcgcatgct cgcccagtcc
     3001 gaaagcctcg ggaaagacca gcaccccggc ccgccgaccg gatgagggtt cgaaaaacaa
     3061 ctggctgcgc atcgtcagac cgtcagcctg ataggtcagt gtttcgtggt gcatccaaca
     3121 gtcccctcta tcaattggat ttcgccgcat cataccgagc aagacgcggc ggctacgcgg
     3181 tgaagggttc aacttcgctt gtcacggcga gccggcagaa cacatggcac gcaagacgac
     3241 ggtcaacgct gatggtttgg ccacgctgat ggtttagtag gtcaatctcg tcgccatgtt
     3301 cgggcgtcag acaatgcggc gcccgtgagg ccggcgcccg tcagctccgc ccgcgtcagc
     3361 cagcactcgt cagcccagcg cccgtcattc cagcaacagt caccgccagc cggtcgccca
     3421 tgcggcgcgg tattgcaacg tccggccatc gcgccaggtc acgccgggcg agcaccgcag
     3481 gcaacaccgc tgcgaccgcc atccgcggaa cagcgatccg gcgagcctcg gcaagcaatg
     3541 cctgaccctc ccggaccacg tccaggagcg cagcccgagc gggttcggac ccgggatcgt
     3601 ggatgaaggc ctcgggcgag acgccgtgcc gccgcaacag gtcgtgcggc aggaggcagc
     3661 ttccctgcga cgccagcacg ccgcttgccc gcagcaatcc ggcgacgcca taggcggcac
     3721 cgaacggacg aaccgcctcg gcctcgggcg cccccagtgc gcccgccgcg gccaccgcca
     3781 gtccccctgc tcccgccaac acccaggcgc gccattcctc tgtcgtctcg aaatcgccgt
     3841 aaacttcggt ctcgcgcgcc tcgatcagcg gcaacagatc agcaggctgc agagtaccag
     3901 cagagattgc gtcaacgagc ggcgttgcca cctcgtgccg cttgcggtcg ccctccacca
     3961 cctcccgcca ccattgcagg cggatcagcg ccaacggagc ctggctggcg acttcgcgcg
     4021 cccgcgccag ttcgtggttg aacgcataca gcgtcagcaa cgcatcccgc cggtcgggcg
     4081 ccgcgaacag cgccgtcaga aaccggtcgg gatcatggcg gcgaaccaag gcggcgatca
     4141 cgtcgttcat gcccaggtcg tggcgcgctt caccagcccg cgcaaccctc ggggccaacc
     4201 tttggttgcc cgagggcgat gccctccgac gtgggtgtcc caattagcgt tctccgacag
     4261 agcatcaatg acgaaaatgg ccagaccccc cgccggagcg gcaacgccct ccggctgcca
     4321 gggggagtcg accacccgcg acggtcggtc gcgcgactct gccggaaccc tgcttcgggc
     4381 ggacctccgt caaagggcac cgccctcggg caaccgaagg ttggcccaag ggttgacgcc
     4441 ttggcgggct ggc
//
LOCUS       DASWQN010000705         3716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1122992, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000705 DASWQN010000000
VERSION     DASWQN010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122992"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VGC82_11970"
     CDS             <1..305
                     /locus_tag="VGC82_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424028.1"
                     /translation="SRWDAAAIGDVVIPACEAGLAHRRGQHARVVTLLEPRLDQIRLL
                     GGSNAQRDLFRQILVDSAMKADRRDVVASLIADEAASHKVPPTQRAGYAAAARWLM"
     gene            319..702
                     /locus_tag="VGC82_11975"
     CDS             319..702
                     /locus_tag="VGC82_11975"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEY0424029.1"
                     /translation="MPVIERLMLDGQPEPVSHYCHVTKAGNLIWVSGTVGVAADGSVP
                     EGVVEQFEVAIANMDACLKAVGAGAEHVVKVVVYLTDIHDRRKINPIRQRYFGPHRPA
                     STLVEVSALVSPELKVEIEAQAVLP"
     gene            complement(779..1822)
                     /locus_tag="VGC82_11980"
     CDS             complement(779..1822)
                     /locus_tag="VGC82_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0424030.1"
                     /translation="MSMNASEPRPYLHPHVNEAWLGKLREEILEPDLPIVDAHHHLWE
                     RESGRYLLDELRADLGAGHNVRGTVFIQCGYAYRGDGPAEMRPVGETERVASIAAQAV
                     SAGSPGVCAGIVGYCDFRLGDRVDAVLEAHVAAAHGRFRGIRQSAGWDAAIVSTTSTV
                     PPRGLLMDPAFRAGLTRLGKFGLSYECSLYHPQLPELTDLARAFPDLPILANHCGGPI
                     RIGPYADHPDDAFAAWRTALRDLAACPNVFLKLGGQAMTIRGFAWHEAPLPPSSEELA
                     AAWRPTMETCIETFGASRCMFESNFPVDKGMCSYVAIWNAFKRVSAGYSPDEKAGLFH
                     DTATRFYRLTLSN"
     gene            2004..2828
                     /locus_tag="VGC82_11985"
     CDS             2004..2828
                     /locus_tag="VGC82_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0424031.1"
                     /translation="MTTSSRVALITGCGKPVGIGNSTAWALAASGIAVVVSDVAPTGV
                     ANEHNTQGDVDPSWGGVDSLVESIRSSGGDAFATLGDVRQEEDAARMVAEVLRRHGRL
                     DILVNNAGAPQGADRNEIENVPVEAWDLTMGVNAKGAFLMCRAAVPVMRKAGWGRIIS
                     VSSRAAFRPGPRRATYAASKAAIVGFTKSLALDLAPFGVTVNAVLPGPIRTTRAISTN
                     RREYGDDLEVGFRERAKEIPVGRFGSPEEVAATIAFLASDGASFVTGQAIGVDGGW"
     gene            3108..3680
                     /locus_tag="VGC82_11990"
     CDS             3108..3680
                     /locus_tag="VGC82_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424032.1"
                     /translation="MMLTSADPHSIEFFVQNARFMPPQDVEDRALRHCADYGLVSRRT
                     QAEWINDWSMTFRFECDDPHKPPSETLADRKAAEPRVAPHKASAIRSPADRKQAAWNQ
                     ANAMSPLWAKCILDEATRMGRSSNERSDIAATTVAASCSHWERDIHEVLRKAGEQDVE
                     FQADLHKQVIEFATSKIVSVRESVPVAASE"
BASE COUNT          677 a         1147 c         1245 g          647 t
ORIGIN      
        1 catcccgctg ggacgcggcg gcgatcggcg atgtcgtcat accggcatgc gaggccggat
       61 tggcgcaccg gcgcgggcag catgcccggg tcgtcaccct actggagccc cgtctggatc
      121 agatccgcct gcttggcggc agcaacgccc agcgcgacct gttccgccag atcctggtgg
      181 attccgcgat gaaagccgat cggcgcgatg tcgtggcgtc gttgatcgcg gatgaggccg
      241 caagccataa ggttccaccg actcaacgtg ccggctacgc cgccgccgca cgctggctga
      301 tgtgaaggaa aacaagccat gccggtaatc gaacgcctga tgctggacgg ccaaccggaa
      361 ccagtcagcc actattgcca cgtcaccaag gccggcaatc tgatctgggt ctcgggcacc
      421 gtgggagtcg cggcggacgg cagcgtcccg gagggtgtcg tcgagcaatt cgaggtcgcc
      481 atcgccaaca tggatgcttg cctgaaagca gtcggtgcgg gcgcggagca tgtggtgaag
      541 gtggtcgtct atctgacgga tatccatgat cgcaggaaga tcaatccgat ccggcagcga
      601 tatttcggcc cgcaccgtcc cgcctcgacg ttggtcgagg tttcggccct ggtctcgccg
      661 gaactgaagg tggagatcga ggcccaggcg gtcctgccgt agcacgcagt tcccgtccac
      721 ggcccaagct cagttcggcc ccgggctcag ttcggcaagg ctcaatttga cgcaaggctc
      781 agtttgacaa ggtcagtcga tagaaccggg ttgcggtgtc gtgaaacaga ccggccttct
      841 cgtcaggcga gtatcccgcc gacacgcgct tgaacgcgtt ccatatggcc acgtagctgc
      901 acatgccctt gtccaccggg aaattgctct caaacatgca gcgggaggcg ccgaaggttt
      961 caatacaggt ttccatggtg ggccgccatg cagcggccag ttcctccgac gacggcggaa
     1021 gcggtgcctc gtgccatgcg aagccccgaa tcgtcatcgc ctggccaccc agcttcagga
     1081 acacgttcgg gcaggccgcc agatcgcgca acgccgtgcg ccaggccgcg aacgcgtcgt
     1141 ccgggtggtc ggcgtagggc ccgatgcgaa tagggccgcc acagtggttc gccaggatcg
     1201 gcaggtcggg gaacgcgcgg gcaagatcgg tcagctccgg tagctgcgga tgatataaag
     1261 aacattcgta actaaggccg aactttccga ggcgcgtaag cccggcgcgg aaggctggat
     1321 ccatcagcag gccgcgcggc ggcacggtag aggtggtgga cacgatggcg gcatcccagc
     1381 cggcgctctg gcgaatgccg cggaaccgcc cgtgagcggc ggcgacatgc gcctccagca
     1441 ccgcgtcaac acggtcgccg agacgaaaat cgcagtagcc cacgataccg gcgcagacgc
     1501 ccggcgaacc ggccgacacg gcttgggcgg cgatcgaggc cacacgttcg gtctcgccca
     1561 ccgggcgcat ttcagcgggg ccatcgccgc gatacgcgta tccgcactgg atgaacacgg
     1621 tgccgcggac attgtgcccg gctcccagat cggcgcgaag ctcgtccagc aggtagcggc
     1681 cggactcgcg ctcccacaga tgatgatgcg catcgacgat cggcagatcg ggttccagga
     1741 tctcctcgcg cagcttgccc agccacgcct cgttgacgtg gggatgcagg tacgggcggg
     1801 gttcgctggc attcatggac atggtggcct cctccgaact ccgcctcgcc cgatcgcgcc
     1861 gacggattgc gccgagcctg ccacggagat ggtgagttaa acaggtgcat gcggtcacgg
     1921 ttatccgccg gaaccctcgc cagcctgcgc ctggcgcgat attctgctga caacaagggg
     1981 ctgacaacaa aggggagact tccatgacga cttccagccg cgttgcgttg atcactggct
     2041 gcggcaagcc tgtcggaatc ggcaattcca ccgcttgggc gttggccgct tcgggcattg
     2101 ccgtcgtggt ttccgatgtc gcgcccacag gcgtcgcaaa cgagcacaac acgcaggggg
     2161 acgtcgatcc gtcgtggggt ggggtggaca gtctggtgga gtccatccgc tccagcggcg
     2221 gcgatgcctt cgcgaccctg ggcgatgtcc ggcaggaaga ggatgctgcc cgcatggttg
     2281 ccgaggtact caggcgccat gggcggctgg atatcctggt caacaacgcc ggcgcgccgc
     2341 agggcgccga ccgcaacgag atcgaaaacg tccccgtcga agcctgggac ctgacgatgg
     2401 gcgtgaacgc caagggcgcc ttcctgatgt gccgagcggc cgttccggtc atgcgcaagg
     2461 cggggtgggg ccggattatc agtgtgtcgt cccgagccgc atttcggccg ggcccgcgtc
     2521 gcgccaccta tgctgcgtcg aaggccgcca ttgttggctt caccaagtcg ctcgcacttg
     2581 atcttgcgcc atttggcgtc accgtgaatg cggtgctgcc cgggccgatc cgtaccacgc
     2641 gggcgatcag caccaatcgc cgcgagtacg gcgacgacct ggaggtcggg ttccgcgagc
     2701 gtgccaagga gattccggtt ggccgcttcg ggtcaccgga ggaggtcgcg gcgacgatcg
     2761 cattccttgc atcggacggc gcgagctttg tcaccggtca ggcgatcggc gtggacggcg
     2821 gctggtagta cattccctgg tggtacgtcc tgggcgcggc caagaatagc ggttgccggg
     2881 gctacgggca tcgggcctga ggacgtaacc gcgttggacc gttggcaatc cccctcctgc
     2941 aacaccagta tgtgcgttga aaacccgatg cttgttagcg acgatgggga ttagcgacaa
     3001 gaggggcgcg gtcattgaag cgatgtgcga tgctggctgg cccaacgttg ctcggcccaa
     3061 tgttgctggg ctcaatgttg ctggcgggct gtggtggacc gccacgagtg atgctgacgt
     3121 cggccgaccc gcactcgatc gagttttttg tccagaacgc caggttcatg ccgccacagg
     3181 atgtcgagga tcgcgcgttg cggcactgcg ccgattacgg gttggtctcg cgccgtaccc
     3241 aagccgagtg gatcaacgac tggtccatga cgttccgctt cgagtgtgac gatccgcata
     3301 aaccgccctc ggaaaccctg gcagacagga aggccgccga accacgggtt gctccgcata
     3361 aggcgtctgc catcagaagc ccggcggatc ggaaacaagc tgcgtggaat caagccaacg
     3421 ccatgtcccc gctctgggcc aaatgcatcc ttgacgaggc tacgcgaatg ggacgttcct
     3481 cgaacgagcg ctcggatatc gcggcgacca ccgtggcggc tagctgctcc cactgggagc
     3541 gcgatattca cgaggtgctg cggaaagccg gggaacagga cgtcgaattc caggcggacc
     3601 tgcataaaca agtgattgag ttcgccacat cgaagattgt ttcggtgcgg gagtcggttc
     3661 ccgtcgctgc cagcgaatga ttttccgata gcgaattggc tgccggaagt tgtgcg
//
LOCUS       DASWQN010000706         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1388193, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000706 DASWQN010000000
VERSION     DASWQN010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388193"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..>1611
                     /locus_tag="VGC82_11995"
     CDS             193..>1611
                     /locus_tag="VGC82_11995"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0424033.1"
                     /translation="MATIQAKSVRRREDFRLLTGQGNYAADAAPGGMAVAIFLRSPHA
                     HARIEHIDAAPARGLPGVIAVYTAADLAGVPPIAGGIGFPRPDGGPAPKTDRPLLAGD
                     RVRFVGEPVALVIAESRAAGLEAAEAIVVDYAGLPVVTEPAAAMAVGAPAVWDDVPDN
                     IGFLWQRGDAEGADAALASSAHVTSLDFTVSRVTANSMEPRGAWAQVAADGRIELHAS
                     HQSPFALRNGIAGGIFQIPPTDIRVVPGDVGGSFGMKSGVHLEPVLAAWAARELNRPV
                     RWISDRTEGFLTDEQAREMLITASLGLDAEGKFTALKLRWNVNLGAYVSGRSGWGVGN
                     IGGIAGVYMIPFIAAQVCGVMTHTVPTAAYRGAGRPEATYAIERLIDVAARELGISPY
                     ELRRRNLIPPAAMPYKTALTFTYDCGEFEGNMQEATALADFATFEERRQAAAKLGKLR
                     GIGLCNCIEVAGGPFLRPAKDLA"
BASE COUNT          262 a          549 c          527 g          273 t
ORIGIN      
        1 ccaggcgata cgaggattga tttcgacccg aacgcggccg attactgatg acatgaaccg
       61 tgtccagccc gcgatgagcc acccacgcga gttggacacg gttcaaattc cctctttcaa
      121 attccctctg cggaatcgcg cttcccgccg aacccgctat gatcgggtga agacaaagcg
      181 ggagctaaga ttgtggcaac gatacaggcg aaatccgtcc ggcggcgcga agacttcaga
      241 ttgctgaccg gccagggcaa ttacgcggcc gatgccgcac cgggtgggat ggcggtcgcg
      301 atcttcctgc gttccccgca cgcccatgcc cgcatcgagc atatcgacgc cgcccccgcg
      361 cgcgggctgc ccggcgtgat tgcggtctat acggctgccg accttgccgg cgttccaccg
      421 atcgccggcg gcatcggctt cccgcgaccc gacggcggcc ccgccccaaa gaccgaccgc
      481 ccactgctgg ccggcgatcg agtgcgtttc gtcggcgaac cggtggcgct ggtcatcgcc
      541 gagtcgcggg ccgcggggct ggaagccgcc gaagcgatcg tcgtcgatta cgccggcctg
      601 ccggtggtga ccgaacccgc tgccgcgatg gccgtaggcg cgcccgccgt gtgggacgac
      661 gtgcctgaca acatcggatt cctgtggcag cgcggcgacg ccgaaggcgc cgacgcggcc
      721 ctggccagtt cggcgcatgt aaccagcctc gacttcacgg tttcccgggt gaccgccaac
      781 tcgatggagc cgcgcggcgc ctgggcccag gttgccgccg acgggcggat cgagctgcat
      841 gcgtcgcatc aatccccctt cgcgctgcgc aacggcatcg ccggcggcat attccagatc
      901 cctcccacgg atatccgcgt ggtgcccggc gatgtcggtg ggtcgttcgg catgaagtcc
      961 ggcgtgcatc tggaaccggt cctggccgcc tgggctgccc gcgaactgaa ccgtccggtg
     1021 cgatggattt cggatcggac cgagggtttc ctgacggacg agcaggcccg cgagatgctc
     1081 atcacggcct cactcggttt ggacgccgag ggcaagttca ccgcactgaa gctgcgttgg
     1141 aacgtcaatc tgggggctta cgtctccggc cggtccggct ggggcgtcgg caatatcggc
     1201 ggcatcgccg gtgtctatat gatcccgttt atcgccgccc aggtttgcgg cgtcatgacc
     1261 cacacggttc ccactgcggc ctatcgtggg gcagggcggc cggaagcaac ctatgcgatc
     1321 gagcggctga tcgatgtcgc cgcccgtgaa ctgggcatca gcccgtatga attgcgccgc
     1381 cgcaacctga tcccgccggc ggcgatgccg tacaagaccg cgctgacctt cacctacgac
     1441 tgcggcgaat tcgaaggcaa catgcaggaa gccaccgcgc tggccgactt cgcgaccttc
     1501 gaggaacgcc gtcaagccgc tgcgaagctc ggtaagctgc gcggtatcgg cctgtgcaac
     1561 tgcatcgagg tcgccggcgg tccgttcctg cgtccggcca aggacctcgc g
//
LOCUS       DASWQN010000707         3146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1651827, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000707 DASWQN010000000
VERSION     DASWQN010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651827"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..950)
                     /gene="upp"
                     /locus_tag="VGC82_12000"
     CDS             complement(321..950)
                     /gene="upp"
                     /locus_tag="VGC82_12000"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY0424034.1"
                     /translation="MPNVTVLDHPLLRHKLTLLRDKTTTTAMFRQVAREISLLMAYEV
                     TRDLPLEPIQIETPLEQMQAQRLAGKKLCLVSILRAGNGILEGMLDLVPSARVGHIGL
                     YRDPATLQPVEYYLKLPNDITDRLVILVDPMLATGHSAAAAVARLKQAGVPAMKFVCL
                     LAAPEGIATFTREHPDVPVFTGAIDRELDSHGYIRPGLGDAGDRFYGTR"
     gene            complement(1003..1809)
                     /locus_tag="VGC82_12005"
     CDS             complement(1003..1809)
                     /locus_tag="VGC82_12005"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0424035.1"
                     /translation="MAVSLRRTLLLGVLVLFILFLYGPTLTILVLSFQGPDGGLTFPM
                     NGFSVHWFARLFQSQAVGDFGGSLIRSFALATMVMVTTVVVSLAAGMAFRRRFRGAGP
                     LFTLTVASLIIPSILVSLGVGLLFNELGLDPDWYSSAFGAQLTWTLPFGLLIMFAVFN
                     RFNPAWEEAARDQGATAWQSFRHVVLPIIAPSVIGIALFGFTLSYDEFARTLLTAGSD
                     NTLPLEIFGMTTSVTTPVLYALGTLTTVFSFVVILCSLVIVRLLAAKRVT"
     gene            complement(1809..2645)
                     /locus_tag="VGC82_12010"
     CDS             complement(1809..2645)
                     /locus_tag="VGC82_12010"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0424036.1"
                     /translation="MAPFLQVAPLAAILCVFLLLPMGVLLVVSFFDYDSVQIIPSFQF
                     TNYTDVLGSAVTWRTYLNTLRYTVIVWAVTAVLGFTIAYVLAFEIRSRTTQMVLFLVC
                     TVPFLTSNIIRMISWIPFLGREGLLNSVLLKAGLIHQPLEFLLFSDFAVVLAYTHLYA
                     LFMVTPIFNTMMRIDRRLLEAALDNGAGWWRTLSSVILPLCKSGIAIGSIFVVTMVMG
                     DFVTVRLMSGGQSASVGLMISSQISSLQYPAACANAVVLLAIVLMMIAAMLRIVDIRK
                     EL"
     gene            complement(2711..>3146)
                     /locus_tag="VGC82_12015"
     CDS             complement(2711..>3146)
                     /locus_tag="VGC82_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424037.1"
                     /translation="PAVAAVRSKGIACTYQPLAEGYRAWAGGLGLAKHLSGLELDAAY
                     EYINWYLSGWVGAYLNRQGYYSAVLDTAKQFMTPDEWGYWIEGKPAQKDVLNPDGKIM
                     EKAGAVRDGGSFTERMGRVACWNSVMNEDRYMIQKWNEFIAA"
BASE COUNT          717 a          950 c          929 g          550 t
ORIGIN      
        1 taacttcttc gtattcctca gcgtccgtgc catggtctaa cctgataaca atgcggagtg
       61 aactggcgct cgaccaattc ttgatcaaga tcagctcgtt gacactaaaa gaaagacccg
      121 tcaacgcttt cgctggcagt cgaatgacag gcatcacacc ctcgctaaaa ctcggactat
      181 cacgatgacg tgaatgtagc tcacgaactt gtgatgatca aggaaattgt tactaattcc
      241 gtgaaggcat cggaatcggc gcaaggctcc ctggcgcaag gctgtcgcgc agttcccgcc
      301 caagtgaaga ttttgccgga ctaccgtgtc ccgtaaaagc gatctccggc atccccgagc
      361 cccgggcgga tgtagccgtg gctgtccaat tcgcgatcga tcgcgccggt gaagactggc
      421 acatccggat gttcccgggt gaacgtggcg atgccttcag gtgccgccag caggcaaacg
      481 aatttcatcg ccggcacgcc tgcctgcttc agccgggcaa ccgcggccgc ggccgaatgg
      541 ccggtcgcca acatcgggtc caccaggatc accaagcggt ccgtgatgtc gttcggcagc
      601 ttcagatagt attccactgg ctgaagcgtc gccggatcgc gatacaagcc gatatggcca
      661 acccgagcag acggaaccag atccagcatg ccttcgagta ttccgtttcc ggcccgcagg
      721 atggaaacca ggcacagctt ctttcccgcc aaacgttggg cttgcatctg ttccaaaggg
      781 gtttcgatct ggatcggctc cagcggcagg tcgcgggtga cctcgtaggc catcagcagg
      841 ctgatctccc gcgcgacctg gcggaacatc gccgtcgtcg tggtcttgtc gcgcagcaac
      901 gtcagcttgt ggcgaagcaa cggatgatcg agcacggtca cgttcggcat gcgcgtgttt
      961 ccccctatag gccgccactc ctggtgctga tgtttccgga cgctacgtca ctcgtttggc
     1021 ggccagcaac cggacgatca ctaacgagca aagaataacc acgaacgaga acaccgtcgt
     1081 caacgttccc agcgcataaa ggaccggggt cgtaacactg gtcgtcatgc cgaagatttc
     1141 cagtggcagc gtgttgtcgc tgccggcggt cagcagcgtg cgggcgaact catcatagga
     1201 cagcgtgaaa ccgaacaacg cgataccgat gacgctagga gcgatgatcg gtagcacgac
     1261 atgacgaaac gattgccacg ccgtggcgcc ttggtcgcgg gcggcttcct cccacgctgg
     1321 attgaagcga ttgaacaccg cgaacatgat cagcagcccg aatggaagcg tccacgtaag
     1381 ctgcgcgccg aacgcagaac tataccagtc gggatcgagg cccagttcgt tgaacagcag
     1441 accgacgccc aggctaacga ggatcgacgg gatgatcagg ctggcaacgg tcagggtgaa
     1501 cagcgggccg gccccccgga agcgccggcg gaacgccatt ccggcggcga gcgagaccac
     1561 aacggtcgtc accatcacca tggttgccag ggcgaatgac cggatcagcg atccgccgaa
     1621 gtcgccgacc gcctgcgact ggaacaacct ggcaaaccag tggacggaga acccgttcat
     1681 cggaaatgtc aggcccccgt ccgggccctg gaacgacagc accaggatag tcagcgtcgg
     1741 cccgtaaagg aacaggatga acagcaccag cacgcccagc aagagagtgc ggcgaaggct
     1801 gacagccatc aaagctcctt ccggatatcg acgatgcgca gcatcgccgc gatcatcatg
     1861 agcacgatcg ccagcagcac caccgcgttg gcacaggccg cgggatattg cagcgacgat
     1921 atctggctgg agatcatcaa gccgacggat gcgctctgac cgccgctcat gaggcgaacg
     1981 gtgacgaaat cgcccatgac catggtgacg acaaagatcg aaccgatggc gatgccggat
     2041 ttgcacagag gaaggatcac cgaactgagc gtccgccacc agccggcgcc gttgtcgagt
     2101 gcggcttcca gcagacggcg atcgatccgc atcatggtgt tgaagatcgg cgtgaccatg
     2161 aacagggcat aaagatgcgt ataggcgagc acgacggcga agtcggagaa caacaggaac
     2221 tccaaaggct ggtggatcag gccagctttc agcaggacgc tgttcaacag accctcgcgc
     2281 cccaggaacg gtatccagga aatcatgcga atgatgttgg aggtcaggaa tggaacggtg
     2341 cacacgagga acagcaccat ctgggtcgtc cggctgcgga tttcgaacgc cagcacatag
     2401 gcgatggtaa agcccagaac ggcggtgaca gcccagacga tgacggtgta gcgcagtgta
     2461 ttgagatagg tgcgccaggt aaccgccgac ccaagcacat ccgtatagtt cgtgaactgg
     2521 aagctcggga taatctggac gctgtcgtag tcgaagaagc tgaccaccag cagcacgccc
     2581 atcggtagca gcagaaagac gcacaggatc gcagccaggg gtgccacttg aagaaatgga
     2641 gccattttga ggcgcgtcac cctgttgatg ccgcgccgcg ccttcagccg gccatcgcgc
     2701 acgggtcagc tcaagccgcg ataaattcgt tccatttctg gatcatgtag cggtcctcgt
     2761 tcatcacgga attccaacag gcaacccggc ccatccgttc ggtaaaggag ccaccgtcac
     2821 gcaccgcgcc ggccttctcc ataatcttgc catcagggtt aagcacatcc ttctgagcgg
     2881 gcttgccttc gatccagtat ccccattcat ccggtgtcat gaactgtttc gcggtgtcga
     2941 ggacagcgct gtagtacccc tgtcggttca aataggcgcc gacccagccg gacaggtacc
     3001 agttgatgta ttcgtacgcc gcgtccagtt ccaggccgct caggtgtttc gccaggccca
     3061 gtccgccggc ccaggcgcgg tagccctctg ccaaaggctg gtaggtgcag gctattccct
     3121 tcgaccgcac ggccgccacg gccggc
//
LOCUS       DASWQN010000708         3436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1784080, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000708 DASWQN010000000
VERSION     DASWQN010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784080"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VGC82_12020"
     CDS             <1..909
                     /locus_tag="VGC82_12020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0424038.1"
                     /translation="PVIATALLHKHRAGLWILTTLACLAVVLGAFFPAIGSDLPDVIG
                     NRILSIVAIVATAAFVHHARDIQDRLTTLTRRAEAAERIKTEVLTDLSREIRTPLHTL
                     LGVLTLTMISSKPEQREVLGRVRNDGKQLLATIDNLIDLTQIEERKLRRQTIDIALIA
                     RDAAASAGPSARDNQVTVALADVGEAAETTAIGDSWAIRRILDNFVANAVRFTPPGGT
                     VSLSVNRLADTVTASVSDNGKGLPSELTQFFHEDAPTTEGSAVSSTGGAGLALSSRLA
                     RTMNGKVIAHNQPGTGAMVSLSLPAA"
     gene            1465..2016
                     /locus_tag="VGC82_12025"
     CDS             1465..2016
                     /locus_tag="VGC82_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0424039.1"
                     /translation="MSTEKSSIHARLAHVGIYAHDKPRLEQFYTTVLGLMVTDSGQAR
                     SGMELTFMSASPGNHHQLVLVSGRPDTSGFNPINQISFMVDSLADLRVVHRRALDNGA
                     TDMRVTSHGNAWSCYFKDPEGNTVEAYLDTPFHVPQPHGEPLDLEKSDDEIMRETEAS
                     CRADPGFMMMDEFQKSLASRLAH"
     gene            2176..2967
                     /locus_tag="VGC82_12030"
     CDS             2176..2967
                     /locus_tag="VGC82_12030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY0424040.1"
                     /translation="MTVSRPLSFILAASEQGTLIVNRHDQHVNSSGGYGVGFEILENG
                     AYNQSEVDLVLRLLNWRREFHGDGVLALDCGANIGVHTIAWAKHMTGWGSVLAFEAQE
                     RVFYALAGNICLNNCLNARALQVAVGDQDGTLSIPVPDYNRPGSFGSLELVFNDNTEN
                     IGQPIDYRQEALQPVPARRLDDLRPPRVDLLKIDVEGMEMSVLRGAMDIVGRCQPYML
                     IEHIKTDQAALAALLDESGYRTWTVGINTLALPKDDPCASRIVVR"
     gene            complement(3005..>3436)
                     /locus_tag="VGC82_12035"
     CDS             complement(3005..>3436)
                     /locus_tag="VGC82_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY0424041.1"
                     /translation="DAMAKGAGVPILHIVEATAADLRRIGISRGTIGVLGTAATLAMG
                     LFQERLSVAGWDTIVPNPREMDRLVSPAIASVKANRVAEAYPPLVEVVNDLAARGATA
                     VVLGCTEIPLGIQAGPADALRVPVVDTIDALARAAIGWATG"
BASE COUNT          681 a         1083 c         1048 g          624 t
ORIGIN      
        1 ccggtcatcg cgaccgcgct gcttcacaaa catcgcgccg gcttgtggat actgactacg
       61 ctcgcttgcc tcgcggttgt gctaggcgcc tttttcccgg cgatcggctc cgatctgccc
      121 gatgtgatcg gcaaccgaat cctttcgatt gttgcgatcg tggcaacggc ggcgtttgtt
      181 caccatgccc gcgacatcca ggaccgattg accaccctga cccggcgggc ggaagcggcc
      241 gaacggatca agacagaggt tctgacggac ctgagccgtg aaatccggac gcccctgcac
      301 acgctgcttg gcgtgctgac cctgacgatg atcagcagca aacctgaaca gcgggaggtg
      361 cttggccggg tgcggaacga cggcaagcag ttgctggcga cgatcgacaa cctgatcgac
      421 ctgacgcaaa tcgaagagcg caagctgcgc cggcagacga tcgacatcgc gctgatcgca
      481 cgggatgccg ccgcaagcgc gggtccgtca gctcgggaca accaggttac tgtcgcgctg
      541 gccgacgtag gagaggctgc cgaaaccacg gcgatcggcg attcctgggc aatacggcgg
      601 attctagaca acttcgtggc caacgccgtc cgattcacgc cgccgggcgg gacggtgtcg
      661 ctatcggtga accggctcgc ggacacggta acggcatcgg tcagcgacaa cggcaaaggc
      721 cttccgtccg aattgacaca gtttttccac gaggacgccc cgacgaccga aggcagtgcg
      781 gtatcgtcaa ccggtggagc cggactggcg ctcagcagcc gtcttgcgcg cacgatgaac
      841 ggcaaggtca tcgcccacaa tcaaccgggg accggcgcga tggtcagtct ttccctaccg
      901 gcggcataac ccgagcattg atcccgcttc tcgattaaat tgggaccagg aagacgacag
      961 aaaaaacccc gtctataagg acagggcttt gatcgatcct gaggtgcaga agttaagatt
     1021 atcgacggtc tttagtcccc ttgaggggcg cttcttctac cgtcgtcaac gaatgccaca
     1081 ggtgatcatg cgctattcaa taccggccta aacaaaccct gtcaagaagc tgatcgttgc
     1141 agagcccgcc gtacccgcat tgttattcgc ctgcccctac tctacccctt tgggccctta
     1201 ctctacccct ttggccccca ctcaacccct ctggagcgca ggtttacgca tgtgtaaagt
     1261 ggcctgggtg gtcggctgag ctgatattgc cggaccggcg gagtcgcgtt cccggagcca
     1321 ctgcaccgtg atcagaatcg agattgcctg gcgacatcgc tgcggcatga ccagaaggga
     1381 caacacaccg aagtccggcc gcccgacgct tgcacaagcg gccaccggga acttgaatgg
     1441 cgtaaggcaa acgagggagc aaccatgtcc accgaaaaat cgagtattca cgcccgtctc
     1501 gcccatgtcg ggatctacgc ccacgacaag ccaaggcttg agcagttcta cacgacggtg
     1561 cttggtctga tggtgaccga cagcgggcag gcccgcagcg ggatggagct gacattcatg
     1621 agcgccagcc ccggcaacca ccatcagctt gtgctggtga gcggccggcc ggatacctcg
     1681 ggcttcaacc cgatcaacca gatttccttc atggtcgact cgctggccga tctgcgggtc
     1741 gtgcatcggc gcgcactgga caacggtgcg accgacatgc gcgtcacctc ccacggcaac
     1801 gcctggtcct gctacttcaa ggatcccgag ggcaataccg ttgaagccta tctcgatacg
     1861 ccgtttcacg tgccgcagcc gcatggcgag ccgctggacc tggaaaaatc cgacgacgag
     1921 atcatgcgcg aaaccgaggc atcgtgccgg gcggatcccg ggttcatgat gatggatgag
     1981 tttcagaaat cgctcgcgag tcgtttggcg cactgagggt ttggctgggc ctgttcgatc
     2041 atcgcttctc gcggacacgt gatacatctt tgactctggc gcatgcgggg ctataccggc
     2101 cggaccatcg accgtacagc ccgcgtcggc acggcggatc acgcgccttg ccgacaggcc
     2161 aggagtgtcc tcgcgatgac cgtgagccgg ccgctatcct ttatactggc ggcctcggaa
     2221 caaggcacgt tgatcgtcaa tcgtcacgat cagcacgtca atagttcggg tggctatggc
     2281 gtcggcttcg aaatcctcga gaacggcgcg tataatcaat ccgaagtgga tctggtcctg
     2341 aggctgctga actggcgcag ggaattccat ggcgacggcg tgctcgcgct cgattgcggt
     2401 gcgaacatcg gtgttcacac catcgcctgg gcaaagcaca tgaccggctg gggtagcgtc
     2461 ctcgcgttcg aggcgcagga acgggtcttc tatgccctgg caggaaacat ctgcctgaat
     2521 aattgcctga atgcgcgcgc cttgcaggtc gcggtcggcg accaggacgg cacattgtcc
     2581 atcccggtgc ccgactataa ccggccgggc agcttcggca gcctggagtt ggtgttcaac
     2641 gacaacaccg aaaacatcgg ccagccgatc gactatcgcc aggaagcctt gcaaccggtt
     2701 ccggcacgcc gattggacga cctccgcccg ccgcgggtcg atttattgaa aatcgacgtc
     2761 gagggaatgg agatgtccgt actgcgcggt gccatggaca tcgtcggccg gtgtcagcct
     2821 tacatgctga tcgaacacat caagaccgat caggcggcgc tggcagcgtt gctggacgaa
     2881 agcggctacc gcacatggac ggttggcatc aacaccctcg cacttccaaa ggacgatcca
     2941 tgtgcgtcgc ggatcgtcgt gcggtgagcg cggccgtcgg cggcatcatc gaccggttgc
     3001 cgtatcagcc ggttgcccat ccgatcgctg cccgcgcgag ggcgtctatc gtatccacta
     3061 cggggactcg tagagcgtcg gccggaccag cctgaatacc gagcggtatt tcggtgcagc
     3121 caagcacgac ggcggtggcg ccgcgagccg cgagatcgtt caccacctcg accagcggcg
     3181 gataggcctc cgcgacgcgg ttcgccttca ccgatgctat cgccgggcta accaggcgat
     3241 ccatttcccg gggattcggc acgatggtgt cccaccccgc gacgctaagc cgctcctgaa
     3301 acagccccat ggcgagggtc gcggcggtcc ccagaacgcc gatggtgccg cgcgagatcc
     3361 cgatgcgccg cagatcggcc gcggtcgcct cgacgatgtg caggatcggc acgccggcgc
     3421 ccttggccat cgcatc
//
LOCUS       DASWQN010000709         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_596426, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000709 DASWQN010000000
VERSION     DASWQN010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596426"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VGC82_12040"
     CDS             complement(<1..179)
                     /locus_tag="VGC82_12040"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008853830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY0424042.1"
                     /translation="MAVVQGLQGIREAYTFDDVLLKPGLSDILPSEADIRSRVTRAIG
                     LNIPIIASAMDTVTEA"
     gene            complement(350..598)
                     /locus_tag="VGC82_12045"
     CDS             complement(350..598)
                     /locus_tag="VGC82_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424043.1"
                     /translation="MIYILAALVPPLGLLLNGQPFAAIFNLVLIVFCLIFGLVFHILL
                     LVPSAHAVIAVHMKREQRRHREVVEAIRQHGPPPGYSR"
     gene            723..2198
                     /locus_tag="VGC82_12050"
     CDS             723..2198
                     /locus_tag="VGC82_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY0424044.1"
                     /translation="MNKERLIPLIVATALFMENMDSTVIATSLPAIAADIGTSPLTLK
                     LAITSYLLSLAVFIPASGWTADRFGARMVFSLAIAVFMVGSVGCALSSSVTHFVIARI
                     VQGVGGAMMTPVGRLVLLRSIDKSALVNAMAWVTVPALVGPVIGPPLGGFITTYFSWH
                     WIFLINIPIGLLGIFMAMRYIDPIRSEDPERFDLYGLVLAGIGLGGIAFGLSVAGLNL
                     LPWSVVAALVAIGIIAMTLYVIHARRTASPVLDFSMLRMPTLRAAIIGGFLFRLGIGA
                     LPFLLPLLMQVGFGLSPFRSGLVTFGSAVGAMGMKTLAARLIRTFGFRNIMMINAVVS
                     SIFLAACALFTSATPLLLIVIILVVGGFFRSLQFTAINTVAYAEIEPAQMSRASTLVS
                     VNQQLAISTGVAVGAFAVETTMMWRHVEELSASDFAPAFIVVSIISAVSTYFFWQMPD
                     DAGHEISGRKAVEISSRKGAAKAAAKAASETTEDARDQRLG"
     gene            2336..>2635
                     /locus_tag="VGC82_12055"
     CDS             2336..>2635
                     /locus_tag="VGC82_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY0424045.1"
                     /translation="MLAAVALCSMCLCARAGTFDDLWLTRIKPPPYGGVATQFTDTIA
                     ADGKRLHPYRESICAHMLERFGQRLRVTNGANGRSVVCRVADKGPNRRFWPRREVD"
BASE COUNT          432 a          855 c          845 g          503 t
ORIGIN      
        1 gcttcggtga cggtgtccat ggcggaggcg attatgggaa tgttgagccc aatcgcccgg
       61 gtgacgcggg agcggatatc ggcctcggag ggcaggatat ccgacaggcc gggcttcaga
      121 agcacgtcgt cgaacgtgta ggcttcccga atcccctgca gtccctgcac aacagccatc
      181 accaactcca ttcggcggcc cgttcgccgc ggtacgacct cgggatgagc aggtcctgcg
      241 gcggcacagc tggcgtcgcc gtcgaatcaa agcccatcgg gtagggttgg cggtggtcga
      301 tagcatggcc ccagcatgat tcaaaggggc cgcggcctca aatccggctt cagcgcgaat
      361 aacccggtgg cgggccgtgc tggcggatgg cctccaccac ctcccggtgc cggcgctgtt
      421 cgcgtttcat gtgaaccgcg atcaccgcgt gggccgaggg caccagcagc aggatgtgaa
      481 aaaccagccc gaagatcagg caaaagacga tcaggaccag gttgaagatc gccgcaaaag
      541 gctgcccgtt cagcagcagt ccgagcggcg gcaccagggc ggcgaggata tagatcatga
      601 tcggatctcc ggcggcgtta cgcaggattt aggtggtatc ccgggttccg caatagcctc
      661 catggcagtg gagtgataca gccgctactc ccgcccgcat ccaccgcccg ttccggaaat
      721 cgatgaacaa agaacgcctg atcccgctca tcgtggctac cgccctgttc atggagaaca
      781 tggattccac ggtgatcgcc acctcgctac ccgcgatcgc cgccgatatc ggcaccagcc
      841 cgctgacctt gaagctggcg atcacctcgt atctgctgtc gctggcggtg ttcattccgg
      901 cgagcggctg gaccgccgat cgcttcggcg cccgcatggt cttcagcctg gccattgccg
      961 tcttcatggt ggggtcggtc ggctgcgcgt tgtcctcctc cgtcacccat ttcgtgatcg
     1021 cccgcatcgt gcagggggtg ggcggcgcga tgatgacgcc ggtcgggcgg ctggtgctgc
     1081 tacgctcgat cgacaagagc gcgctggtca acgcgatggc ctgggtgacg gtgccggcgc
     1141 tggtcggccc ggtgatcggg ccgccgctcg gcggtttcat cacgacctat ttctcctggc
     1201 actggatctt cctgatcaat attccgattg ggttgctcgg catcttcatg gcaatgcgct
     1261 acatcgaccc gatccgcagc gaggaccccg agcgcttcga tctctacggg ctggtgctgg
     1321 ccgggatcgg gctgggcggc atcgcgttcg ggctctcggt cgcgggcctc aacctgctgc
     1381 cctggagcgt ggtcgccgcg ctggtcgcga tcggcatcat cgcgatgacg ctctacgtca
     1441 tccacgccag gcgcacggcc tcgccggtgc tggacttttc gatgctgcgg atgccgacat
     1501 tgcgggccgc catcatcggc ggcttcctgt ttcgcctcgg catcggcgcg ctgccgttcc
     1561 tgctgccgct cttgatgcag gtcggattcg gcctatcgcc gttcagatcg ggactggtga
     1621 cgttcggctc cgcggtcggc gccatgggca tgaaaacgct cgccgcgcgc ctgatccgga
     1681 ccttcggttt ccgcaacatc atgatgatca atgcggtcgt cagctcgatc ttcctcgccg
     1741 cctgtgcgct gttcacctcg gccacgccgc tgctgctgat cgtgatcatc ctcgtggtcg
     1801 gcggcttctt ccgctcgctg cagttcaccg cgatcaacac cgtggcctat gccgagatcg
     1861 agccggcgca gatgagccgc gccagcacgc tggtcagcgt caaccagcaa ctggcgatct
     1921 cgacgggcgt cgcggtcggc gcgtttgcgg tggagaccac catgatgtgg cgccatgtcg
     1981 aggaactgag cgcgtccgat ttcgcgccgg cgttcatcgt ggtctcgatc atctccgccg
     2041 tatcgaccta tttcttctgg cagatgcccg acgatgccgg ccacgaaatc tccggccgca
     2101 aggcggtcga gatttccagc cgcaagggcg cggcaaaggc agccgccaag gcggcgagcg
     2161 aaactaccga ggacgcgcgc gatcagcggc tggggtagat caggcgtttc gcacccgacg
     2221 cggaacgacg gtggggtgtg gccagaaagc ctctgctgcg gagaaacagg cagtttcgcc
     2281 acgatctcgc caataatccc gccgcacaat tcccgccatg tatgtacgtg cgtcaatgct
     2341 cgcggcagtc gcgttgtgtt caatgtgcct gtgcgcacgc gccggcacgt tcgacgatct
     2401 ttggctgacc cgcatcaagc cgccgcctta cggcggcgtc gcgacccaat ttaccgatac
     2461 gattgcggcg gatggcaagc ggctgcatcc ctaccgggag tcgatctgtg cccacatgct
     2521 cgaacgcttc gggcagcggc ttcgcgtcac caatggtgcg aacggcagat ccgtcgtctg
     2581 ccgggtggcc gacaagggac cgaaccgccg attctggccg cgccgcgaag tcgac
//
LOCUS       DASWQN010000710         5765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2311489, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000710 DASWQN010000000
VERSION     DASWQN010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2311489"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /gene="msrA"
                     /locus_tag="VGC82_12060"
     CDS             complement(<1..553)
                     /gene="msrA"
                     /locus_tag="VGC82_12060"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY0424046.1"
                     /translation="MSVTQETATLGGGCFWCLEAVFKDLRGVSWVMSGYAAGSVPNPS
                     YKAVCGGRTGHAEVVQVKFDPNELAYADLLRVFFSIHDPTTQDRQGNDVGSQYRSIIL
                     THSEAQAETARAVMKEISDAKIWPGKLVTQVEPLTVFYEAEAEHHDYFARNPWSGYCQ
                     VVVAPKVVKFRKQFADRVKRDAAE"
     gene            766..1335
                     /locus_tag="VGC82_12065"
     CDS             766..1335
                     /locus_tag="VGC82_12065"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEY0424047.1"
                     /translation="MSELMEKAHETIEKHASHSDPWARGVALLVSFLAAALAITQIGG
                     NAAQNAYLTHHVSLSNDWAFFQAKNLRAVVRTSEADLLASLPNATDPAIQARIKDATD
                     YSARMRDDPVGGDGMKQLAAKAQAQEHDRDEAAHRYHNYEYAAGALEIAIVLASVSIV
                     TRIRALTIGAGVIGAAAAVAAIGVASNLF"
     gene            1397..1909
                     /locus_tag="VGC82_12070"
     CDS             1397..1909
                     /locus_tag="VGC82_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424048.1"
                     /translation="MRKTFEPIDDLLIERLFQPVLDMVAHRTGLSRPAACCFCVDMAS
                     LAWIVSRVRELSDAIGKWDLGSAFVDVAILMLGLVALACLRTLFRRAGNKQANPLRPA
                     MQPHRAVMLLMLAARVVQLRVPTLMAAADLSMLLFAASALYLGACVERPPMRRGFALH
                     HARVDVAVSP"
     gene            complement(2057..2719)
                     /gene="aat"
                     /locus_tag="VGC82_12075"
     CDS             complement(2057..2719)
                     /gene="aat"
                     /locus_tag="VGC82_12075"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY0424049.1"
                     /translation="MSRRQVEVTTDLVLRAYRHGLFPMAEGRDGDKLFWLDPIKRGVL
                     PLHEFHLSRRLLRTVLSGGFTVTADRNFAGTIAGCATPVPDRPDTWINPQIERLFAEL
                     FRMGHAHSVETWQGSTLVGGLYGVALGGVFFGESMFSFARDASKVALVHLIARLRLGG
                     FRLLDTQFITDHLAQFGAEEIPRERYKQLLADAVDEPAIWLSDPEQETLTREFRRIAG
                     RD"
     gene            2764..3177
                     /locus_tag="VGC82_12080"
     CDS             2764..3177
                     /locus_tag="VGC82_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0424050.1"
                     /translation="MLTIKHLDHLVLRVTDLRTMMAFYTDVLGCPVEKVQEDLGLYQL
                     RAGSALIDLVPVDGPLGAKGGAAPGPEARNLDHFCLRVEPFDSSAIIAHLRAHGAEPA
                     PVASRYGAEGQGPSIYVSDPEGNVVELKGPPGPVA"
     gene            complement(3193..3840)
                     /locus_tag="VGC82_12085"
     CDS             complement(3193..3840)
                     /locus_tag="VGC82_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424051.1"
                     /translation="MRLIWRFSLSVSVLAFVCGCGPNYSPDTYASNAVQQANKVEQGV
                     VVGVRNVGVSASGTTGTITGAAAGGIAGSQAPVGPMGALTALGGSLVGGIAGATVEHA
                     TADTAAFEYIVRKQAGDLVSVTQKDKTPLALGQKVLVIAGNQARVVPDYTLPPDVSPN
                     KSAETAKPVETAGAVVMPKAEALPAPVDTKPVETGPGDAKAADRVPNQPEDPATH"
     gene            3932..5686
                     /locus_tag="VGC82_12090"
     CDS             3932..5686
                     /locus_tag="VGC82_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY0424052.1"
                     /translation="MSNTPPGRAPSTEQDARTMQGAVAKHGLARNTANYGDKDFALYL
                     RRSFAKSMGYSQAMLDRPVVGIVDTGSAYNNCHRTVPELIDAVKRGVLAAGGLPMPFP
                     TISLCEPSLFPTSMHYRNLAALDTEAMLTAQPMDAAVLIGGCDKTVPAQLMAAASADV
                     PAVQLVTGPMLATPFQGERLSACTDCRRYWAQYRAGTVTEQRIGELEGRLATTAGTCG
                     VMGTASTMACIAEALGMIPLGHGSIPAVHADRLRAAEEAGALAVRLIENPIRPSQIMT
                     AHAVENALRVLLALGGSTNALIHLTAVAGRLGVKIDLRKLNALSDSTPVLVDLKPTGD
                     GYMEDFHAAGGMPALLWELRDLLHMDPIDVTGVTLRERLTEPAFVDRRYIRTVREPVS
                     PVGGIVSLFGSLAPRGAILKRSAATPSLFEKEARAVVFDGLEDLANRIDDPDLDVTAE
                     DILVLKNAGSLTPAGMPEAGYLPIPSRLARAGVKDMVRMSDCRMSGTAFGTIVLHITP
                     EAAAGGPLGLVESGDRIRLSVKDRSLDLLVSAEELERRQASWVPPAKPKRGWDRLIAD
                     QVLQADEGCDLAVLRAEG"
BASE COUNT         1024 a         1782 c         1862 g         1097 t
ORIGIN      
        1 actcggccgc gtctcgtttg acccggtcag caaactgctt gcggaatttc accacctttg
       61 gcgctaccac cacctggcaa taaccgctcc agggattgcg tgcgaagtag tcatgatgtt
      121 cggcctcggc ttcgtagaac accgtcaacg gttcgacctg ggttaccagc ttgccgggcc
      181 agattttagc gtcgctgatt tccttcatca ccgcccgcgc cgtttcagcc tgcgcctcgg
      241 aatgcgtcag aatgatcgag cgatactgcg aaccgacgtc gttgccctgc cggtcctgcg
      301 tggttggatc atggatcgaa aaaaacactc gtagaagatc cgcgtaggcc aactcgttcg
      361 ggtcgaactt gacctgcacg acctcggcat gtccggtccg gccaccgcag acggctttgt
      421 agctgggatt gggcacgctg ccggcggcat agccggacat cacccagctg actccgcgaa
      481 ggtccttgaa gaccgcctcg aggcaccaga agcatccgcc gcccaaggtg gccgtttcct
      541 gcgtcacgct catgtccatc tccctctcgg tgacctgatc atatatgcgc tttcgccttc
      601 cgtcacaccg gcacccggcc gctcggcctt ttcgcggcat ccgagaccgc catcgatcgg
      661 cgcgaacagg cacgccgtcg gccaggaatg cccgcgggac tttcatttgg tgattttgcc
      721 tatctgtgaa caaacatggc ggcggcagcc acaggagagc tcgacgtgag tgagttgatg
      781 gaaaaggcgc acgagacgat cgagaaacac gccagccact ccgatccctg ggctcgggga
      841 gtcgccttgc tggtgtcctt cctcgccgcg gcgttggcga taacccagat cggcggcaat
      901 gccgcgcaga atgcctattt gacgcaccat gtctcgctat cgaacgactg ggcgttcttc
      961 caggccaaga acctgcgcgc ggtggtgcgg acttcggaag ccgatctgct ggccagcctg
     1021 cccaatgcga ccgacccggc gatccaggcg cggatcaagg acgccacgga ttacagcgcc
     1081 cggatgcggg acgatcccgt ggggggcgac ggcatgaaac agttggcagc caaggcacag
     1141 gcccaggaac acgatcgcga cgaggccgca caccgctacc acaactatga atacgcggcc
     1201 ggcgccctgg agatcgccat cgtcctggca tccgtttcga tcgtaacgcg catccgggcg
     1261 cttaccattg gtgccggcgt gatcggcgcc gctgcggctg ttgccgcaat cggtgttgca
     1321 tcaaacttat tttgatccat tgacattatg ccgcattacc tgctttaggg tcacataatc
     1381 gtgaaggtga ccttaaatgc gaaagacgtt cgaaccgatc gacgatttgc tgatcgaacg
     1441 gctttttcaa ccggtgttgg atatggtcgc ccatcgcacc ggcctcagcc ggccggcggc
     1501 gtgctgtttc tgcgtcgata tggcatcctt ggcctggatc gtgtcgcggg tgcgggaatt
     1561 gtcggacgcg atcggcaagt gggatctggg ttcggccttc gtcgacgtcg cgatcctgat
     1621 gttgggcctt gtcgctctgg cttgcctgcg cacgttgttt cgacgggctg ggaacaaaca
     1681 ggccaatccg ctgcggccgg caatgcagcc gcatcgggcg gtcatgctgc tgatgctcgc
     1741 cgcgcgcgtg gtgcaacttc gggtgccaac gctgatggcg gcggccgatc tttcgatgct
     1801 gctgtttgcc gcatccgcgc tttaccttgg tgcctgcgtc gaacggccgc ctatgcggcg
     1861 cggtttcgct ctccaccatg cccgagtgga cgtagccgtt tcgccgtaat gacggcgatt
     1921 aatcgggcta agggtccgtc gagcgcaatc ggcgattgac catcttcagc ctgactgtgt
     1981 cagccagaaa cggtctatct cccgctgcat cgcttgatta acgcgcttcg gacgttccgc
     2041 ccagcgtgat cacgctctag tcccggccgg cgatccgccg gaactcccgc gtcagcgtct
     2101 cctgttccgg gtcggaaagc cagatcgcgg gttcgtccac cgcgtcggca agaagctgct
     2161 tataacgttc gcgtgggatt tcttcggcgc cgaactgtgc aaggtggtcg gtgatgaact
     2221 gggtatccag caaccggaag ccacccaggc gcagccgggc gatcaagtgt actagcgcga
     2281 ccttggacgc gtcgcgggcg aagctgaaca tgctctcacc aaagaacacg ccgccgagcg
     2341 cgacaccgta caagccgccg accagtgtgc tgccctgcca ggtttccacc gaatgggcgt
     2401 gccccatgcg gaaaagttcc gcgaacaagc gctcgatctg cgggttgatc caggtatcgg
     2461 gccggtcggg caccggtgtc gcacagccgg cgatggtgcc ggcgaagttg cggtcggcgg
     2521 tgacggtgaa gccgccggac aggaccgttc ggagcagccg gcgggacaag tggaactcgt
     2581 gcagcggcag cacaccgcgt ttgatgggat ccaaccagaa cagcttgtca ccgtcgcgcc
     2641 cctcggccat cggaaaaaga ccgtggcgat aggcgcgcag cacaaggtcc gtcgtgactt
     2701 ccacctggcg tcgggacatg gtcgtatagt gctgcgtggg gcgacggatt gaaaggacga
     2761 acgatgctga cgatcaagca cctcgatcat ctggttcttc gggtcaccga cctgcggaca
     2821 atgatggcgt tctacaccga tgtgctgggc tgtccggtgg aaaaggtcca ggaggacctt
     2881 gggctgtacc aattgcgggc aggcagtgca ttgatcgacc tcgtaccggt ggacggaccg
     2941 ttgggtgcga aaggcggtgc ggcgccaggc ccggaggccc gcaacctgga ccacttttgc
     3001 ctaagggtgg aaccgttcga ctccagtgcc atcatcgcac atttacgcgc ccatggggcc
     3061 gaacccgctc cggtggcgtc ccggtacggc gccgagggcc aggggccgtc gatctacgtt
     3121 tctgatccgg aaggcaatgt cgtggagctt aagggtcccc cgggcccggt ggcctgatcc
     3181 gtcggggaaa catcaatgcg tcgccggatc ttccggctgg tttggcaccc ggtccgcggc
     3241 tttggcatcc cctggcccgg tttcgaccgg cttggtgtcc accggtgcag gcaaggcttc
     3301 ggctttcggc atgacgacag ccccggccgt ctcaaccggt tttgcggttt cagccgactt
     3361 gtttggggac acatccggcg gcagcgtgta atccggtacg acccgcgcct ggttccccgc
     3421 gatcaccagc accttctgcc caagcgccag gggcgtcttg tccttctgtg tcacgctgac
     3481 cagatcgcca gcctgcttgc ggacgatgta ttcgaaggcg gccgtgtccg cggtagcatg
     3541 ttcgacggtc gcgcccgcta taccgcccac cagcgaacca cccaacgcgg ttaaagcacc
     3601 catcggaccg acaggagcct gggatccggc gatgccgccc gcggccgcgc cggtgatggt
     3661 gccggtcgtg cccgaggcgc tgacgcccac attgcgcacg cccaccacga cgccctgttc
     3721 gactttattt gcttgctgca ccgcgttgct cgcataggta tcgggggagt agttcggtcc
     3781 gcatccgcac acaaaggcca gaaccgatac cgagagggaa aaccgccaga tcaaacgcac
     3841 gctcctgtcg ttgcaggcgt gtggtagccg atgatgccaa ccgagtcaca gcagtttgag
     3901 tcatgtcggc gaccgggcta ggatggcaga catgtcaaat acccctccag gacgcgcccc
     3961 ctcgaccgag caggacgcaa ggaccatgca gggcgctgtt gccaaacacg gtctggcccg
     4021 caacaccgct aactacggcg acaaggattt cgcactttac ctccggcggt cgttcgcgaa
     4081 atcgatgggc tattcccagg cgatgctgga tcgtccggtg gttgggatcg tcgataccgg
     4141 atcggcctac aacaattgcc accggacggt gccggagctg atcgacgcgg tgaagcgcgg
     4201 ggtcctggcc gccggcggac tgccgatgcc atttccgaca atttccttgt gcgagccgtc
     4261 gctatttccg acctcgatgc attaccgcaa tctcgcggca ctcgataccg aagcgatgct
     4321 gacggcgcag ccgatggacg cggcggtgct gatcggcggc tgcgacaaga cggtgccggc
     4381 ccagctgatg gcggcggctt cggcggatgt cccggcagtg caacttgtaa ccgggccgat
     4441 gctggcgact ccgttccagg gcgagcgact gtcggcctgc accgattgcc ggcgctactg
     4501 ggcgcaatac cgtgccggta cggtcacgga acagcggatc ggggagcttg aaggccggct
     4561 ggcgacgacc gccgggacat gcggcgtgat gggcactgcg tccaccatgg cctgcatcgc
     4621 cgaggctttg ggtatgatcc cgctgggcca tggctcgatc cccgcggtgc acgccgatcg
     4681 gctgcgtgcg gcggaggaag ctggcgcctt ggcagtgcga ctgatcgaga atccgatccg
     4741 gccgtcgcag atcatgaccg ctcatgcagt cgaaaacgcc ctgcgcgttc tgctggcctt
     4801 gggcggatcg accaacgcgc tcatccacct gacggcggtt gccgggcgat tgggcgtaaa
     4861 gatcgatctg cgcaagctga acgcgctgtc cgattcgacc ccggtgctgg ttgacctgaa
     4921 gccgaccggc gacggctaca tggaggattt ccacgccgcc ggcggaatgc cggcgctgct
     4981 gtgggagctg cgggacctgc tgcatatgga cccgatcgac gtcaccggcg tcaccctgcg
     5041 ggagcgcttg actgaacctg ccttcgtgga cagacggtat attcgcaccg tgcgcgaacc
     5101 ggtatctccg gtaggcggta tcgtgtcgct gttcgggtcg ttggcgcctc ggggagcgat
     5161 ccttaagcgg agtgcggcca ccccgtcttt gttcgaaaaa gaggcgcgtg ccgtggtgtt
     5221 cgacggcttg gaagacctgg ccaatcggat cgacgatcca gacctggatg tgaccgccga
     5281 ggatatcctg gtgttgaaga acgccgggtc gctgacgccg gcgggcatgc cggaagccgg
     5341 ctatctgccg atcccgtctc gcctcgcccg cgccggagtg aaggacatgg tgcgcatgag
     5401 cgattgccgc atgtcgggaa cggcgttcgg cacgatcgtg ctgcacatca cgccggaagc
     5461 cgccgccgga gggcctttgg gactggttga aagcggcgac cgtatcaggc tgtcggtgaa
     5521 agaccggtcg ctggatctgc tggtgtcggc agaggaactg gagcgccggc aagcatcatg
     5581 ggtgcctccg gccaagccga agcgcgggtg ggaccggcta attgccgatc aagtgctgca
     5641 agcggacgaa ggctgcgatc tggcggtgct gcgggctgag ggatgatgtg gcaaggctac
     5701 gttgaacctg aactggggtt ggatatgagc gtcacgcttc agaaaccgat gagcgttgac
     5761 gaatt
//
LOCUS       DASWQN010000711         3450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1256494, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000711 DASWQN010000000
VERSION     DASWQN010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256494"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..413)
                     /locus_tag="VGC82_12095"
                     /pseudo
     CDS             complement(188..413)
                     /locus_tag="VGC82_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612190.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(435..1217)
                     /locus_tag="VGC82_12100"
     CDS             complement(435..1217)
                     /locus_tag="VGC82_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0424053.1"
                     /translation="MAIRKLLLPLISTSAGEAALSTAWTVAQLWNAHMLVLHVQMDLA
                     PAPAMSAMFQRFTVEHRVRVAEATTGAESATASFLISTGRKLDILPQQARLADLTVVR
                     HPATPEEVSSSDVLHAVLFDSGRPLLIAPQVAPTTVGRRICLGWNGSAESAAAVEAAM
                     PWLERAEAVCILWAKEYQRHGPQAPELQAHLAMHGVSADLRMFKPIDNIVGAGLLAAA
                     KEFNCDLLAMGAYAHSRLRQQILGGVTRHILEHATLPVMMHR"
     gene            1555..1854
                     /locus_tag="VGC82_12105"
     CDS             1555..1854
                     /locus_tag="VGC82_12105"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEY0424054.1"
                     /translation="MQMRIIWGKILPGQWGAFEAAFKAALEIRGEVKGLKGQWLLQDQ
                     NDPDAGYSISQWESEEDMRAFWDSKKRGEAMAVLQPFYVNQFTITNCAVRIASSY"
     gene            2042..2245
                     /locus_tag="VGC82_12110"
     CDS             2042..2245
                     /locus_tag="VGC82_12110"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEY0424055.1"
                     /translation="MAVASVSKITAASPTSFDAAIQEGLARANRTLRGITGLHVIEEK
                     ASVKNGKIEEYRVTMEVTFILEE"
     gene            2443..>3450
                     /locus_tag="VGC82_12115"
     CDS             2443..>3450
                     /locus_tag="VGC82_12115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424056.1"
                     /translation="MGVRSLALLDRDACTLPHLRGCSGQRKPKRQNRGKPMTTALEVR
                     TMRKVYLHLLPFTFALYLICYLDRANIGFAALTMNRDLGLSSYIYGLGAGAFFWGYFL
                     LEVPSNLILERVGARRWIARIMITWGIVSASFAFVSGPISFFILRFLLGLAEAGFFPG
                     MILYFTYWFPPYHRARIIAGFMAAIPVAIGLGAPVSTALLELDGIFGLAGWKWLFLAE
                     ALPALILGGAVLFYLTDRPEVANWLAPDEKQWLTSVMAEERRRVEAEPRVSVWRSMID
                     RRVLALAAIHFAQAGVSVSMAVFIALMIKGLGLTNMQTGWLTSVPFIFGTIGILVWGH
                     IS"
BASE COUNT          684 a          976 c         1057 g          733 t
ORIGIN      
        1 caagtcccag cagatgcgat ggtcgcgaaa aggcgccgat ctgctgctcc aaatccgctg
       61 tgcagtctat aacggcacgc tcggtaccgg gtttgggcat cgatttgacc ggacctccaa
      121 cgcggacccg acctttgcta aaacggcatg atcctccaat gtttgggcag tcccgacgaa
      181 cactcgacaa agccttgaac aggatggtac tttcagcatc ggcaacggac cagccgacca
      241 cgcggcggct gaagatgtcg atggtgacgt atagatagaa gtaggtccat cttgctggcc
      301 ccttcagttt ggtgatgtcc acgaccggct tcgttggggg ctaccgccag caattccggt
      361 ttggtgtagg caggatggtg caactgccgg cggcgtacgc aaacctcgct gtggcatgga
      421 ggatgcgatg cggcctaccg atgcatcatg accggcaatg tcgcgtgctc caggatgtgc
      481 cgcgtcacgc cgccaagaat ttgttgccgc aaacgggagt gggcataggc gcccatggcc
      541 agaaggtcgc agttgaactc tttggccgcc gccagcagac cagcgccgac tatgttgtcg
      601 attggtttga acatcctcag atcagccgac acaccatgca tcgccaagtg ggcctgaagc
      661 tctggcgctt gcggaccatg tcgttgatac tctttagccc agagaatgca cacagcttct
      721 gcgcgctcca gccaaggcat tgccgcctcc acggcggcgg cggactcagc cgagccattc
      781 cacccgaggc aaatccgcct gccgactgtc gtcggcgcca cctggggggc aatcagcagg
      841 gggcggccgg aatcaaacag caccgcgtgt agaacgtccg aggaggacac ctcttctggg
      901 gtagcgggat ggcgcaccac cgtgaggtcg gcgagccttg cctgctgcgg gaggatatcc
      961 agcttccgtc ccgtcgatat gagaaagctg gcggtggcgc tttccgcccc agtggtggct
     1021 tccgccactc tcaccctgtg ctcaacagtg aagcgttgga acatcgcgct catcgctggg
     1081 gccggtgcca gatccatttg cacgtgtagc acgagcatgt gcgcgttcca gagctgggcg
     1141 acggtccagg cggtggacag agccgcctct cccgcactgg tgctgatcag tggcaataaa
     1201 agcttgcgga tggccatgat ctacactccg ataagccctg ccttacgtag cataaagccg
     1261 cgcacgagtt gtccgaaacg ttcagagccg acaccaggct gtgaagaatg agctgccaaa
     1321 caggcgctcg gcggttccct gtggttgccc aggctttggg tcgtggtctc cgcctgagca
     1381 ctttcgtgct gtatttgtcc cagaccatcc acttcggaca atttcgcaaa ttgtttgtaa
     1441 ccgcgcgccg ctgacgataa ggtatcggtg ggcacttggg gctgataggc catctgggca
     1501 atgcccgtgg tcacttgcaa cacgcgtcgc cgaggcccga tagggggtat cgtcatgcag
     1561 atgcggatca tctggggcaa aatcctgccc ggccaatggg gtgctttcga ggctgccttc
     1621 aaggcagcgc tggaaattcg cggggaagtg aagggactga aaggtcaatg gctacttcaa
     1681 gaccagaacg atcctgatgc cggatattcg atctcgcaat gggaaagtga ggaggatatg
     1741 cgggccttct gggacagcaa gaagcgcggt gaagccatgg ccgtgcttca gccgttctac
     1801 gtgaaccagt tcacgatcac caactgcgca gttcggatag catcttcata ttaatcggac
     1861 ggtggcgcca cccgcttcgg cgacctgctg taccaacccg agaacgcgat acgggcgtct
     1921 ggtggccatt ctcgtaggcc acagagcaag cggttgtaga ggcgcgttgg caagctgctt
     1981 gtcggaaacc atactggctt gttggcagcc gagtggcggc caatccttcg aggaggcaga
     2041 aatggcagta gcaagcgtgt caaagatcac cgcggcttca ccgaccagct tcgatgccgc
     2101 tattcaggaa ggcctcgcac gagcaaacag gacattgcgc gggatcactg gcctgcacgt
     2161 gatcgaggag aaggcgagcg tgaaaaacgg caagattgag gagtaccggg tgactatgga
     2221 ggtaaccttc attttggaag aatgagggga tgacctggcg gccacggtaa ccggcccgga
     2281 cgcttcgtgt tgtgtttggc cgtgatcaat cagaagaacg ctatctcgaa gacaagtttg
     2341 agaatgctta tccggcatac cgagcaagaa cccgtcgctg gatttagggg gtcgcatagt
     2401 ccctgagcga ggggcctgcg gtctttttgc tggtcgcccg gcttgggcgt tcgaagcctg
     2461 gcgctactag accgggatgc gtgcacgctg ccgcatctca gagggtgcag cggccaacga
     2521 aagccaaaac gacaaaacag ggggaaaccg atgacgaccg cgcttgaggt caggactatg
     2581 cgcaaggtct acctgcacct tctgccattc acctttgccc tctatctgat ttgctatctt
     2641 gatagagcca atattggctt tgcggcactg accatgaacc gcgatctggg cttaagttcg
     2701 tatatctacg gattaggcgc gggcgctttc ttctggggct acttcctgct ggaggtgccg
     2761 agcaacctga tcctggaacg cgtcggcgca cgccgctgga ttgcccgtat catgatcact
     2821 tggggcatcg tatccgcctc gtttgcattt gtcagtggcc cgataagctt cttcatcctc
     2881 cgtttcctgc tggggcttgc cgaagccggc tttttccctg gcatgattct ctacttcacc
     2941 tattggttcc cgccgtatca ccgggcccgg atcatcgccg gctttatggc ggccattccg
     3001 gtggccatcg gccttggcgc accagtgtcc accgctttgc ttgagctcga tggcatcttt
     3061 gggctggcag gctggaaatg gctgttttta gcagaggcat tgccggcact gatcctggga
     3121 ggcgcggtgc tattctatct gaccgatcgt ccagaggttg cgaactggct cgcgcccgac
     3181 gagaagcagt ggctgacttc cgtgatggcg gaggaacgcc ggcgcgtcga ggcggaaccg
     3241 cgcgtctcgg tctggcggtc catgattgat cggcgcgtgc tggccctggc agcaatccat
     3301 ttcgctcaag ctggcgtcag cgtcagcatg gcggtgttca ttgcgcttat gattaaggga
     3361 cttggcctga ccaacatgca gaccgggtgg ctgacttcag taccgttcat cttcggcacc
     3421 atcggcatcc tggtttgggg tcatatctcc
//
LOCUS       DASWQN010000712         3137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_69150, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000712 DASWQN010000000
VERSION     DASWQN010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69150"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VGC82_12120"
     CDS             complement(<1..840)
                     /locus_tag="VGC82_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435937.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0424057.1"
                     /translation="MSQATATAEHVPTEARIQAKVAALGPWAWLRANLLSSWWSTAVT
                     LALGYIIVRVLFSLFEWGFANAVWTVPYNAAGIPDTSVCQNVKGVGACWAVLTDKYRL
                     ILFGRYPFDEQWRPAIVVVLFIGLYVVSAMRRFWRKELALIWVATLAAVGVLMWGGIF
                     GLTEVTNDNWGGLPLTLILATFGAAFSFPLAILVALGRRSTKLPAVKLLCVLYVELIR
                     GVPLISVLFMASVMFPLFMPAGVNVDKLLRAQIAFILFSGAYLAEVVRGGLQALPKGQ
                     AEAA"
     gene            complement(842..2053)
                     /locus_tag="VGC82_12125"
     CDS             complement(842..2053)
                     /locus_tag="VGC82_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004753.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0424058.1"
                     /translation="MSQATADPRVLTARAAPKRRMRLSWADPVFRSVVWQVVIVGFVV
                     AVCWYLAHNTSENLAARHIATGFGFLDRVAGIPIGESLLPYNPAEDTYGRALLIGVLN
                     TLKVSVIGVILATILGTIIGIGRLSKNWLLAKMTAFYVETLRDLPLLLQLLFWYTVLQ
                     GLPGPKQSWHLGEFAFLSNRGMKLPVLDWQPAHTWALLAFIAGFIAMMMWNRRARKQQ
                     EATGVRPAVWPVALVLLIVLPLIVWAALGAPFQLELPVLRGFNFQGGTTLSPEYFALM
                     LGLIIYTSSYIAEIVRSGIQAIPVGQWEAAGALGLRPSHVLRQIVLPQALRVIIPPMT
                     SQYLNLAKNSSLAVAIGFQDIVSIANTTLNQTGQAIEGIAVIMAVYLTISLSISLFMN
                     WYNARIALVER"
     gene            2284..2814
                     /locus_tag="VGC82_12130"
     CDS             2284..2814
                     /locus_tag="VGC82_12130"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0424059.1"
                     /translation="MSATLKPLTVDEFLAWERAQELRFEFDGIQPVAMTGGSLAHTRI
                     TTRLTIALGQRVQSPCEPLGPELKVLTPGRVRYPDASIICSEPDEDSDIVSPTVVFEV
                     LSPSTVLTDRRVKAMEYAAVPSILAYVMLETDRPEVTVRRRSGGWEAETIAGIEADLD
                     LPEVGVTVPLAEIYLR"
BASE COUNT          612 a          951 c         1065 g          509 t
ORIGIN      
        1 ggcggcctcg gcctgacctt taggcagggc ttgcaggccg ccgcgaacca cctcggccag
       61 gtaagcgccg gagaacaaaa tgaaggcgat ctgcgcccgc agcagtttgt ccacgttgac
      121 gccggccggc atgaacagcg ggaacatgac gctggccatg aacaggaccg agatcagcgg
      181 cacgccgcgg atcaattcca catacagcac gcagagcagt ttcaccgcgg gtagcttggt
      241 agagcggcgg ccgagggcga ccaggatggc cagcggaaaa gagaacgcgg caccgaacgt
      301 cgccaggatc agggtcagcg gcaggccgcc ccagttgtcg ttggtgacct cggtcaagcc
      361 aaagatgccg ccccacatca gcacgcccac ggcggccaag gtcgccaccc agatcagcgc
      421 cagttccttg cgccagaagc ggcgcatggc ggacacgacg tacaggccga tgaacaacac
      481 caccacgatc gccgggcgcc attgctcgtc gaaaggatag cggccgaaca ggatcagccg
      541 atatttgtcg gtcagaaccg cccagcaggc cccgacgccc ttgacgttct ggcaaaccga
      601 ggtgtcgggg atgccggcgg cgttgtacgg caccgtccag acggcgttgg caaaccccca
      661 ttcgaacagg ctgaacagga cgcggacgat gatgtagccc aaggccagag tgaccgcggt
      721 ggaccaccag gaactcagca ggttggcgcg tagccaggcc cacggcccca gcgccgcgac
      781 tttcgcctgg atgcgagcct cggtggggac gtgttcggcg gtggcggtgg cctggctcat
      841 gttatcgctc caccagcgcg atgcgcgcgt tgtaccagtt catgaacagg ctgatcgaca
      901 ggctgatggt aagatagacg gccatgatca cggcgatgcc ctcgatggcc tggccggtct
      961 ggttcagcgt ggtattggcg atcgagacga tgtcctggaa gccgatcgcg acggcgagcg
     1021 aactgttctt ggccaggttc aggtactggc tggtcatcgg cgggatgatc acgcgcaacg
     1081 cctgcggcag cacgatctgc cgcaggacgt ggctggggcg caagccaagc gcgccggcgg
     1141 cttcccattg accgaccggg atggcctgga tgccggagcg gacgatctcg gcgatgtacg
     1201 acgaggtgta gatgatcaga ccgagcataa gggcgaaata ctcggggctg agggtggtgc
     1261 cgccctggaa gttgaagccc ctcaggaccg gcagttcgag ttggaacggt gcgccgaggg
     1321 cggcccagac gatcagcggc aagacgataa gcaggaccag ggccaccggc cagacggcgg
     1381 gccggacgcc ggtggcctct tgttgtttgc gagcgcggcg gttccacatc atcattgcga
     1441 tgaagccggc gatgaatgcc agcagggccc aggtgtgggc aggttgccaa tcgagcaccg
     1501 gcagcttcat gccccggttc gacagaaagg caaactcgcc gaggtgccat gactgctttg
     1561 ggccaggcag gccctgcagc acggtgtacc agaacaacag ttgcagcagc aacggcaggt
     1621 cgcggagggt ttccacatag aaggcggtca ttttcgccag caaccaattt ttcgacagcc
     1681 ggccgatgcc gatgatggtg ccgaggatgg tcgccaggat gacgccgatc accgacacct
     1741 tcagcgtatt cagcacgccg atcagcagcg ctcggccgta agtatcctcc gcggggttgt
     1801 acggcagtaa cgattccccg atcgggatgc cggcgacgcg gtcaaggaac ccaaaacccg
     1861 tggcgatatg gcgggcggca agattctcgg aggtgttgtg ggcgagatac cagcagaccg
     1921 cgacgacgaa gccgacgatg acgacctgcc acaccaccga ccggaacacc gggtcggccc
     1981 aggacaggcg catgcgccgc ttcggtgccg ctctcgcggt caggacgcgg ggatcggcgg
     2041 tggcttgtga catcaggcgg cggtctccct cgctggtggt ctggcagggt aatgacgcaa
     2101 gatcgatgcc atacgagatg cgcacgtcac atggctgcgc tgggagtgtg cggtggatcg
     2161 cacacgggat caaccggcat gtttctggcg aagtgctgga gttcgggtcg tggttcctat
     2221 atcaattgcg cacgcccgtg aggcgcatcg gacacgcccg ggaggcgcat cggaacggac
     2281 tgcatgagcg caacactgaa gccgctgacc gtggacgagt tccttgcctg ggaacgggcg
     2341 caagaattgc ggttcgaatt cgacggtatc cagccggtcg ccatgaccgg gggcagtcta
     2401 gcccataccc gcatcaccac ccgactgacg atcgcgcttg ggcaacgtgt ccaatcgcca
     2461 tgtgaaccgc tcggtccgga gctgaaggtc ctcacgccgg gccgggtgcg gtatccggac
     2521 gcaagtatca tatgctcgga accggacgag gatagcgaca tcgtttcgcc gaccgtggtg
     2581 ttcgaggtac tgtcgccctc caccgtgctg acagatcgtc gggtcaaggc gatggaatat
     2641 gccgctgttc caagcatcct ggcctatgtg atgctggaga ccgaccggcc cgaggtcacc
     2701 gtccgccgcc gatctggcgg ctgggaggcc gagaccatcg ccggtatcga agccgacctg
     2761 gacctgcccg aggttggcgt gacggtcccg ctggcggaaa tttatcttcg gtagatcggc
     2821 gggaatcgcc agacgctgcc ggcgaaggcg gagtcggtcg gcgtgaggcc gtttggaccg
     2881 ctcgtgccct ctggcctggt aatcgttttg tggcgttgca tacgatggta ttctaaggtc
     2941 gcggttccgt gatttcgtcg cagtgcattc ttctgcctgc gacgcctgcc tgggagccga
     3001 agacacagga tggcccgttc aactcgcaca acggcgcgtc ctgccgccgc cacagccgat
     3061 cccgcgaacg cggtcaagga ctaccggcac cggcaggagg cgaccgctcg gccggatgcc
     3121 ggcgcggcgc cccggtt
//
LOCUS       DASWQN010000713         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_333059, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000713 DASWQN010000000
VERSION     DASWQN010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333059"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VGC82_12135"
     CDS             <1..378
                     /locus_tag="VGC82_12135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0424060.1"
                     /translation="GVFVTGRVPDVRPYIAHATAGVAPMRIARGIQNKVLEAMSLGKP
                     VVLTSGALEGIEAEPGKEIILADTIEAFAASCSRLATSNDGASIGAAARTRVLRDYDW
                     SARLRRFDDLLRPMSAGAVTETV"
     gene            424..>1963
                     /gene="xrtA"
                     /locus_tag="VGC82_12140"
     CDS             424..>1963
                     /gene="xrtA"
                     /locus_tag="VGC82_12140"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HEY0424061.1"
                     /translation="MNAPVLTAARPVAARADWRRLLPALITGLLLLGALFSKEVAAAV
                     ETWNASTAYNHCFLVIPIALYLLWDRRFDLTGIAVRPMPSALLLGLPLAAAWLLSERL
                     GIMEGRQIVAVSFVELLFLAVLGKRLWWALAGPLLYLFFLVPFGEFLTPALQDVTTFF
                     IRHGLETLGVPAYIDGYVIEIPQGTFFVAEACASLRFLIASLAFGCLYALMMYRSPMR
                     RGVFILVSIVVPVIANGLRGIGIVYLGYLLGSAQAAAADHVIYGWVFFSAVILLLIAL
                     GLPFRQDDIPTRSSSGQCATAGANAFSIRLAFAIAFGVAGMAILAPTVAAGLTIRAGI
                     AVPTPARIEVGPDCAVQSATQPEAPGSAVMTQRVLCGDVRMDMSWEAFPPRSTAGPIM
                     AERRRLVLRALTEGLSENWLDAGDGVASPWRIMSSNDPAYTTAVSVWIDGKPIRPGLT
                     MRLRMAVASVTGSAYAPMLVIVTPVVDWQTSNTAEIKAAETSLPRFLLTHPGLDRTIG
                     ALSVL"
BASE COUNT          287 a          632 c          650 g          394 t
ORIGIN      
        1 ggcgtcttcg tgaccggccg ggtacccgat gttcggccct atattgcgca cgcgaccgcc
       61 ggggtggcac ccatgcggat cgcccgtggc atccagaaca aggtgctcga ggccatgtcg
      121 ctcggcaagc ccgttgtgct cacctccggc gcgctcgagg ggatcgaggc ggagccgggg
      181 aaggagatca ttctggctga cacgatcgag gctttcgccg cgagttgctc ccggctggcc
      241 acaagtaacg acggggcttc gatcggcgcc gcggcccgta cccgtgtcct gcgagattat
      301 gactggtcgg cgcggttgcg ccggttcgac gacctgctgc gcccgatgtc ggccggtgcc
      361 gtcaccgaaa ccgtttgacg gacagtccat ggccagacgc atcataaccg agggccctgg
      421 ctcatgaacg cacccgtcct gaccgcggcg cgaccggtgg ccgcgcgggc ggactggcgc
      481 cggctactgc cggctttgat cacgggcctg ctgctgctgg gcgcgctttt cagcaaggaa
      541 gtcgccgccg cggtcgagac ctggaacgct tcgaccgcct acaatcactg tttcctggtc
      601 attccgatcg cgctttacct gctgtgggac cgccgcttcg acctgaccgg catcgccgtt
      661 cggccgatgc catcggcgct gctgcttggt ctgccgctcg cagccgcatg gctgctgtcg
      721 gaacggcttg gcatcatgga ggggcggcag atcgttgccg tcagctttgt cgaattgctg
      781 ttcttggcgg tactgggaaa gcggttgtgg tgggccctcg cgggtccgct gttatacctg
      841 tttttcctgg tgccgttcgg ggaattcctg acaccggcgc tgcaggacgt gaccacgttc
      901 ttcatccgcc acgggctcga gacccttggc gttcccgcct acatcgacgg ctatgtcatc
      961 gaaattccgc aaggcacgtt cttcgtcgcg gaggcctgtg ccagcctgcg gtttctgatc
     1021 gcctcgctcg ccttcggctg cctttacgcg ttgatgatgt atcgcagccc gatgcgccgc
     1081 ggcgtgttca tcctggtctc gatcgtcgta ccggtcatcg ccaacgggtt gcgcggcatc
     1141 ggcatcgttt atctcggata tcttctcggc agtgcccagg ccgcggcagc ggaccatgta
     1201 atctatggct gggtgttctt ctcggcggtc atcctgctgc tgatcgcgct gggcctgccg
     1261 tttcggcagg acgatatccc gacccgatcg tcgtcaggac agtgcgccac ggctggagca
     1321 aacgcatttt ccatacgcct ggcgttcgcg atcgcgttcg gggtggccgg catggccata
     1381 ctcgctccga ccgtggcggc cggcctgacg atcagggcag gcatagcggt tccgacgccc
     1441 gcccgcatcg aagtcggccc cgactgcgcg gttcagtccg cgacccaacc cgaggctccc
     1501 gggtccgctg tcatgacgca gcgcgtgctg tgcggcgacg tgcgaatgga catgagttgg
     1561 gaggcattcc caccccgttc caccgccggg ccgatcatgg ctgaacggcg gcggctggtg
     1621 cttcgggcct tgaccgaagg actgtcggag aactggttgg atgccggaga cggggtcgcc
     1681 agcccgtggc ggatcatgag ctcgaacgat ccggcctaca cgaccgccgt gtccgtctgg
     1741 atcgacggca agccgatccg gccgggattg acgatgcggt tgcgaatggc cgtggccagc
     1801 gtgaccggat cggcttacgc gccgatgctc gtcatagtga cgccggtggt ggattggcaa
     1861 acaagcaaca ccgccgagat caaggccgct gaaaccagtc tgccaaggtt cctgttgaca
     1921 catcctggcc tggaccggac gatcggggcg ttgtccgtgc tgc
//
LOCUS       DASWQN010000714         2440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1388841, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000714 DASWQN010000000
VERSION     DASWQN010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388841"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VGC82_12145"
     CDS             complement(<1..588)
                     /locus_tag="VGC82_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY0424062.1"
                     /translation="MPAALGVVTPDAMASIILVLGAPRSGTTWLAKIVDSHPDVLYRH
                     EPDETLPSSGPLTGDMLPALLSRWVGDRGARTVTKRPFFPKSWQSAGARHARAVFAAA
                     VSTASRLPALMNALTRVQIPDLMRCPAPRVAIKSVRWSGGAAILARTLPGSRTVFILR
                     HPCGQVASVIRGNSRRRFDLKTKGTDMPFDEAAAIQ"
     gene            complement(598..888)
                     /locus_tag="VGC82_12150"
     CDS             complement(598..888)
                     /locus_tag="VGC82_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0424063.1"
                     /translation="MMGFRVTIANTGATIDCPTEQQILHSAVAAGVDYPYTCATGNCA
                     SCISELRAGEVSMLPYSDNALSAAQFAEGKILACRARPLSDVEVLWLGRGRR"
     gene            complement(885..1253)
                     /locus_tag="VGC82_12155"
     CDS             complement(885..1253)
                     /locus_tag="VGC82_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424064.1"
                     /translation="MEWGFYLTIALSGKEAGPHDHGIWCVNAAISGREVHKFYRRSDD
                     GSRDGYATVDEIGEVTVSPGHGMAMADHAIHATEVVGNEPAIGLALYGYALARFPSVV
                     WFHPAFDTVRASPSRRMEAA"
     gene            complement(1213..1464)
                     /locus_tag="VGC82_12160"
     CDS             complement(1213..1464)
                     /locus_tag="VGC82_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424065.1"
                     /translation="MNEQRVEACATLIRQARAILDADGSGEAALEKIKGLLVGLADRG
                     ETLFPRGDFAMPDAQGRNHILALEDGDGMGLLPHDRPVW"
     gene            1463..>2440
                     /locus_tag="VGC82_12165"
     CDS             1463..>2440
                     /locus_tag="VGC82_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0424066.1"
                     /translation="MIGPPLSPPSLRDPATQRYSPKMSKRLQQIDAFFRQGQASHLAG
                     RLVEAESIYRQVIAAAPRHADALHALGALALQAGNAAVADTLLTQAITLKPAADFQLT
                     RAAALLALHRPDDAARIARVVLRARPQSAEAHQLLGHALCDSKHANEAVEAYRTALRL
                     RPELPDIRNNLGLALRQAGLLAEAEQEFRQAPAEPEALVNLSSVQKERGAFAEAEATL
                     RRALDIAPANPILRYNWSLLMLLLGRTPDAWDGWEQRFRAGAIPERHFSQPQWHGEPL
                     GNRTLLVYAEQGLGDVIQFVRYLPAIRGNVVFEAPPRLMRLLSANPALPP"
BASE COUNT          447 a          837 c          747 g          409 t
ORIGIN      
        1 ctgaatggcg gcggcctcat cgaacggcat atcggttcct ttggtcttca ggtcgaaccg
       61 ccgccggctg ttgccgcgta tgaccgacgc aacctgaccg caggggtgcc gcaggatgaa
      121 aaccgttcga ctgcccggca gggtccgagc caggatcgcc gcaccgccgg accatcgtac
      181 cgacttgatc gccacccggg gcgcggggca acgcatcagg tccggtatct gaacccgtgt
      241 cagcgcgttc atcagtgcgg gcaggcggga cgcggtactg accgcggcgg cgaacaccgc
      301 ccgcgcatgg cgcgcccccg ccgactgcca cgattttggg aagaacggcc gcttggtgac
      361 ggtgcgtgcg ccgcggtcgc ccacccagcg agaaagcagg gctggcaaca tatcaccggt
      421 caacgggcct gaactgggca atgtctcgtc gggttcgtgc cggtaaagca cgtcaggatg
      481 gctatcgacg atctttgcca gccaggtcgt gccggaacgc ggcgccccta acacaaggat
      541 gatcgacgcc atcgcgtccg gtgtaacgac tcccaacgcc gccggcattt ggatcgatta
      601 ccgccgcccc cggcccagcc aaagaacttc aacatccgat agcggcctgg cacggcaggc
      661 caagatcttt ccctcggcaa attgcgctgc ggacagcgca ttgtcgctgt acggcagcat
      721 cgaaacttcc ccggcgcgca gttccgaaat acaggaggcg cagtttccgg tggcgcaggt
      781 ataaggataa tccacgccgg ctgcgaccgc ggagtgcaga atttgctgtt ccgtgggaca
      841 atcgatcgtc gccccggtat tggcgatggt aacccggaac cccatcatgc cgcctccatc
      901 cgccggctcg gcgacgcccg aaccgtgtcg aaggccggat ggaaccacac caccgaggga
      961 aaccgagcca gcgcatagcc gtaaagtgcc aagccgatgg ccggttcatt gccgacaact
     1021 tcggtggcgt ggatggcgtg atccgccatc gccatgccgt ggccagggga aaccgtcact
     1081 tcgccgattt catcgaccgt cgcataaccg tcgcgcgagc catcatcgct acgtcgatag
     1141 aatttgtgca cctcccgccc ggaaatcgcc gcgttcacgc accagattcc gtgatcgtgc
     1201 ggccctgctt ccttaccaga cagggcgatc gtgaggtaaa agccccattc catcgccatc
     1261 ctcgagagcc aggatatggt tgcggccctg cgcatcgggc attgcgaagt caccgcgcgg
     1321 gaacagcgtc tcgccccggt cggccagccc cacgagcagt cccttgatct tttccaacgc
     1381 cgcctcgccg gagccgtcgg catcgagaat cgcgcgagcc tggcggatca gcgtcgcgca
     1441 cgcttctaca cgttgttcat tcatgatcgg tcccccgctt tccccgccat cattgcgcga
     1501 tccggccacc cagcgctact ccccgaagat gagcaaacgg ttgcagcaga tcgacgcctt
     1561 cttccgccag ggacaagcat ctcatctggc tgggcgtctg gtggaagcgg aatcgatcta
     1621 ccgccaggtg atcgccgcgg cgccgagaca tgcggatgcg ctgcacgccc tcggcgcgct
     1681 ggcgctgcaa gcgggaaacg ccgcggtcgc cgacacgctg ttgacccagg cgattaccct
     1741 gaagcccgct gccgactttc aacttacccg tgccgctgcc ttgctggcac tgcaccggcc
     1801 ggatgacgcc gcacggattg cccgcgtcgt gctccgggcg cgcccgcaaa gcgccgaagc
     1861 ccaccagttg ctcggtcatg cgctgtgcga cagcaagcac gcgaatgagg ccgtcgaagc
     1921 ctaccgaaca gcgcttcgac ttcggccgga actgccggac atacgcaaca acctcggctt
     1981 ggcgttgcgc caggcgggcc tgctggctga ggccgaacaa gagtttcgcc aggcgcccgc
     2041 cgaacctgag gcactggtca atctttccag cgtgcagaaa gaacgcgggg ccttcgccga
     2101 agccgaggcg acgctgcgcc gtgcgctgga catcgccccc gcaaacccga tactgcgcta
     2161 caactggtcg ctgctgatgc tgctgctggg ccgaaccccg gatgcctggg atggctggga
     2221 acaacgtttc cgcgccggcg ctattccgga gcgacatttc agccagccac aatggcacgg
     2281 ggagccgctc ggcaatcgca ccttgctggt atatgcggaa caggggcttg gcgacgtcat
     2341 ccagttcgtc cgctaccttc cggcgatccg cggcaacgtc gtgttcgagg cgccaccgcg
     2401 cctgatgcgc ttgctgtcag ccaatccggc gctgccgccg
//
LOCUS       DASWQN010000715         3960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_861081, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000715 DASWQN010000000
VERSION     DASWQN010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861081"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..2254)
                     /locus_tag="VGC82_12170"
     CDS             complement(176..2254)
                     /locus_tag="VGC82_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY0424067.1"
                     /translation="MKSARLAVDIGGTFTDLALEQGDRRTTTKVLTTPADPERGVMEG
                     FQTIIQEAGVAPGDIGLIIHGTTLATNSIIERKGARTALITTEGFRDVIAMGNESRYD
                     QYDLNIVLPEPLVPRYLRLPVPERLDNTGKVLRPLDEAAVHALISLLRQEQVASVAVG
                     LLHAFVNPVHERRIQAILQQELPDIPVSLSSDVSPEMREWERFSTTVANAYVQPMMAR
                     YLRRLEADLQAAGADAPLFLMMSGGGLTTIETACRFPIRLVESGPAGGAIFSAHIARE
                     CGLDSVLSFDMGGTTAKICLIDDYKPQTARTFEVARVGRFRKGSGLPLRIPVIEMVEI
                     GAGGGSVAHVDTMGRIAVGPESAGADPGPACYGRGGTHPAVTDANLSLGRYDPGRFAG
                     GSMKLDVAAAHDALLADVGAKLGLTAELAALGVVEMVDENMANAARVHAIESGKTYEG
                     RTLIAFGGGGPVHACRVAEKVGINRVLVPSGAGVGSAIGFLRAPVGYEVVRSLYQRFS
                     TFDVAAVNDLLDDMRQEAEAAVEKGSFGAPLVETRTAYMRYVGQGHEIPVPLPNRLLK
                     AEDVALVRARYDEEYARFFDRPVPGSDVEIMSYAVVVTTAVAEISVPLSNENLVPIKA
                     APVRTQLVRDTTTGAVSPWAIYDRMALAPASTIVGPAIVMEDETSTLIGPAWTATVNP
                     LGYIEMVRGR"
     gene            complement(2446..>3960)
                     /locus_tag="VGC82_12175"
     CDS             complement(2446..>3960)
                     /locus_tag="VGC82_12175"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0424068.1"
                     /translation="DPQLNAYLLVVADRALAQARAAEADIAAGRYRGPMHGIPFALKD
                     IYCTAGIRTTCHSRTLADYVPDFDATTVAKLYDAGAVLLGKLSTHEFAHGGPSFDLPW
                     PVARNPWNREHATGGSSSGSGASVAAGLAPGSLGSDTGGSIRNPAALCGLVGLKPTYG
                     LVSRSGVYANSFSYDHAGPMTWTVEDCAIMLQAIAGHDPKDPASAGHAVPDYRAQLSA
                     GVKGLRIGVLRHLYEDDVPVAASLKAALEQAYDVLRGLGATIEDARIRSAADYHAVKI
                     TGAESELFAVHEPALRKRLNDFGADFLGRVLGALLISGPDYMQASRQRRAMIAEMAPL
                     YDRYDVLLTAGPGPAARLDAWRTIDFWQRNSVTTPFNVTGGPALVQCIGFTAEGLPLS
                     MQVVGRPFADAMVLRVAKAYEDATLWRQQRPVLEAGAAPLSAPSVPPPAKGEISQVRR
                     DEIASICKRRGLVVPEKHFEQLCATAPYVEEMVGHLGRSWDFTDEPASVFQFGG"
BASE COUNT          727 a         1362 c         1189 g          682 t
ORIGIN      
        1 gatcgacatg cccttgacga ttagcgcaat gctgctgatc gaagacacga aatataccgt
       61 cctgagacgc ctcaacgaaa cctatgatgc actccccatt gacctgacta cgcggtggcg
      121 gccgtcgtgc tactggtcag atgaggcagc ggaacaggac cttccgcggc ctgtcctacc
      181 gccctcgaac catctcgatg tagcccagtg gattgactgt ggcggtccat gcgggaccga
      241 tcaatgtcga ggtctcgtct tccatgacga ttgccggccc tacgatcgtg gacgctggcg
      301 ccaaggccat ccggtcatag attgcccagg gcgacaccgc acccgtggtc gtatcgcgca
      361 caagctgcgt gcgaaccggc gccgccttga tcgggaccaa gttttcgtta cttaatggca
      421 ccgatatctc ggccaccgcg gtcgtgacga ccacggcgta gctcatgatc tcgacatccg
      481 accctggtac tggccggtcg aaaaacctgg catattcctc atcatagcgg gcgcggacaa
      541 gtgcgacgtc ttcggccttc agcaaccggt ttggcaacgg cacggggatc tcgtggccct
      601 gtccgacata gcgcatatac gccgttcggg tctccaccag cggtgcgcca aagctgcctt
      661 tctccaccgc cgcttccgct tcttgccgca tgtcgtccag cagatcgttc accgccgcaa
      721 catcgaacgt cgagaaccgc tgatacaggc tgcgaacgac ctcatacccc accggcgcgc
      781 gcaaaaaacc gatcgcgctg ccgacgccgg cgccggaggg caccagcacg cggttgatcc
      841 cgaccttctc cgccacccgg caggcatgca ccggcccgcc gccaccgaat gcgatcaacg
      901 tgcggccttc gtaggttttg ccggactcga tggcatgcac gcgggcggcg ttggccatat
      961 tctcatcgac catttcgacc acaccgagcg ccgccagttc ggctgtcaga cccagcttgg
     1021 cgccgacatc ggccagcagc gcatcgtgcg ccgcggcgac atccagcttc atcgagccac
     1081 ccgcgaagcg gccggggtca taccgcccaa gcgacagatt ggcgtcggtc acggccggat
     1141 gcgtgccgcc acgtccgtaa cacgccggcc cgggatcggc gccggcgctt tccggcccca
     1201 cggcgatccg gcccatcgta tcgacatgcg ccaccgatcc gcctccggca ccgatctcca
     1261 ccatttcgat gaccggaatc cgcagcggca gacccgatcc cttgcggaaa cggccaaccc
     1321 gggcgacctc gaacgtgcgg gcggtttgcg gcttgtagtc gtcgatcagg cagatcttcg
     1381 ccgtcgtgcc gcccatgtcg aaggacagca cgctgtccag gccgcattcc cgagcgatat
     1441 gggcggaaaa gatcgcaccg ccggcaggcc cgctttccac cagacggatc gggaagcggc
     1501 atgcggtctc gatcgtcgtc aacccgccgc ccgacatcat caggaacagc ggcgcatcgg
     1561 ccccggccgc ttgcaagtcg gcttccaacc gccgcaggta acgagccatc atcggctgca
     1621 cgtaggcgtt ggcgacggtg gtggagaaac gctcccactc ccgcatctcc ggcgaaacgt
     1681 ccgatgacag cgacacggga atatccggca gctcctgctg caggatcgcc tggatgcggc
     1741 gctcgtgaac cggattgacg aaggcatgca gcaagccgac ggcgacgctg gcgacctgtt
     1801 cctgacgcaa aagcgagatc aacgcgtgca ccgcggcctc atccaatggg cgcaggactt
     1861 tcccggtgtt gtccaaccgt tccggcaccg gcaggcgcag gtagcgcggc accaatggct
     1921 ccggcagcac gatattcagg tcgtattggt cgtagcggct ttcgttgccc atggcgatga
     1981 catcgcggaa accctcggtc gtgatcagcg cggtgcgtgc gcccttgcgc tcgatgatcg
     2041 aattggtcgc aagcgtcgtg ccgtggatga tcaggccgat atcgcccggc gcgacgccag
     2101 cttcctggat gatcgtctgg aaaccctcca tcacgcctcg ttccgggtcg gctggcgtgg
     2161 tcagcacctt tgtggtggta cgccgatcgc cttgttccag ggccagatca gtaaacgtgc
     2221 cgccgatatc gacagcaaga cgagcagatt tcatccaagt ccccggagcg agatgagcga
     2281 tgcgcagcaa agcctagttg cattcgccgc gccttcctat ctgctattgg cgatggcgct
     2341 atgggaaact gatcgatttc ccttatcgat ccctggcgtt gacgcgttac ggccgcttta
     2401 tctgatgctt gaaacaagcg tcaggaacgg atcaacaggc cctacctatc caccgaactg
     2461 gaacacgctc gccggctcgt ccgtgaagtc ccaggagcgg ccgagatgac cgaccatctc
     2521 ctccacatag ggcgccgttg cgcatagctg ctcgaagtgc ttttccggca ccaccaatcc
     2581 gcggcgcttg cagatcgaag cgatctcgtc gcgccgaacc tgactgattt cccctttcgc
     2641 ggggggcgga acggagggcg cagacagcgg tgccgcacct gcctccagca ccggacgctg
     2701 ctgccgccaa agggtggcat cctcgtatgc cttcgccacc cgcagcacca tcgcgtcggc
     2761 gaacggcctt cccaccacct gcatcgatag cggcagacct tcggcggtga atccgatgca
     2821 ctgcaccaat gccggcccgc cggtcacgtt gaatggtgtc gtcacggagt tccgctgcca
     2881 gaaatcgatc gtccgccagg cgtccaaccg tgccgccgga cccggtccgg ccgtcagcag
     2941 gacgtcgtag cggtcgtaca gaggggccat ctcggcgatc atcgcccgcc gctgccgcga
     3001 ggcctgcatg taatcgggtc cgctgatcaa cagcgcgccc agcacccggc ccaggaaatc
     3061 ggcgccaaag tcgttcagac gcttccgcag cgccggctcg tggacggcga acaactcgct
     3121 ttccgccccg gtgatcttca ccgcgtggta gtcggcagcc gaccggatcc ttgcatcctc
     3181 gatcgtggcg ccgaggccgc gcagcacgtc ataggcttgc tccagggcgg ccttcagcga
     3241 ggcggcaacg ggaacatcgt cctcatacag atgccggagc acgccgatcc gcagtccctt
     3301 tacgccggcg gaaagctgcg cgcggtaatc cggcaccgca tgtcccgcgc tggccgggtc
     3361 cttgggatca tgcccggcga tcgcctgcag catgatcgcg cagtcctcga ccgtccatgt
     3421 cattggtccg gcatggtcgt agctgaagct gttggcataa acgccggacc ggctgaccag
     3481 cccataggtc ggcttcaacc ccaccagccc gcatagcgcc gccggattgc ggatggaccc
     3541 gccggtatcc gatcccaggg aaccaggtgc caatccggcg gccacgctgg cgccggaacc
     3601 gctcgacgag ccgccggtgg cgtgctcgcg gttccaggga ttccgagcca ccggccacgg
     3661 caggtcgaac gatggaccgc cgtgggcgaa ctcatgcgtt gacagcttgc ccagcagcac
     3721 cgccccggca tcatatagtt tggcaacggt cgtcgcatcg aagtccggga cgtagtccgc
     3781 caaagtacgt gaatggcacg tcgtccggat gccggcagtg cagtagatat ccttcaaggc
     3841 gaaggggatc ccgtgcatcg gcccgcggta gcggcctgcc gcgatgtccg cctcggccgc
     3901 ccgcgcctga gccagcgccc gatccgcgac aaccagcaga taggcgttca actgcggatc
//
LOCUS       DASWQN010000716         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_464753, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000716 DASWQN010000000
VERSION     DASWQN010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464753"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VGC82_12180"
     CDS             <1..201
                     /locus_tag="VGC82_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HEY0424069.1"
                     /translation="VLEPRLNDIAHEEGYEGRVLIAADPAMTGADCRIEWRGGGAERS
                     ELNIENALSALISRHFSNSVKG"
     gene            287..565
                     /gene="fliN"
                     /locus_tag="VGC82_12185"
     CDS             287..565
                     /gene="fliN"
                     /locus_tag="VGC82_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEY0424070.1"
                     /translation="MPMEAAPKHTAADLEAVFDVPVTVSAVLGKSGMEVSQLLKLGRG
                     TIVELDRKVGEAIDIYVNDRLVARGEVVLVEDRLGITMTEIIKAGSGS"
     gene            591..>1680
                     /locus_tag="VGC82_12190"
     CDS             591..>1680
                     /locus_tag="VGC82_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0424071.1"
                     /translation="MRLLIVGGLQGQIGLATKIAMDKGAKVTHAADIEQALATLRGGI
                     GADLVFCDVGLDIGALVRGLAAERICTPVVACGIGTDTRRAVDAIRAGAKEYLPLPAD
                     PELIAAVLAAVADESHQLVYEDEAMAAVLSLADQIAGSEASILVTGESGTGKEVLARY
                     VHRKSARNDKSFISVNCAAIPENLLESELFGHEKGAFTGAIARRIGKFEEANGGTLLL
                     DEISEMDARLQAKLLRAIQEREIDRVGGTKPVKVDIRIIATSNRDLAEAVKHGTFRED
                     LLYRLNVVNLRIPALRERPKDIRALSHHFARKYAEANGVPYRAISSPTERLLLGHYWK
                     GNVRELENTIHRAVLLATGPDISPEAIRL"
BASE COUNT          330 a          536 c          522 g          292 t
ORIGIN      
        1 gtgctggaac cccggctgaa cgatatcgcc catgaagaag gttatgaggg ccgcgtgctg
       61 atcgccgccg accccgccat gaccggcgcc gattgccgca tcgagtggcg cggcggcggc
      121 gccgaacgca gcgaactcaa catcgaaaat gcgctgagcg cccttatttc ccgtcatttc
      181 tcgaattcag tgaaaggata gtctcatggc gaccgacaat gtcgatctgc ccgatctcag
      241 ccaggaaggc gcggccagtc cctccggcgc gctcctcaac accgacatgc cgatggaggc
      301 cgcgcccaag catacggcgg ccgacctgga ggcggtgttc gatgtcccgg tcacggtcag
      361 cgccgtgctg ggcaagagcg ggatggaagt cagccagctg ctgaagctgg gccgcggcac
      421 cattgtggaa ttggatcgca aggtgggcga agccatcgac atttatgtca atgaccgcct
      481 ggtggcgcgc ggcgaagtcg tgctggtgga agaccggctg ggcatcacca tgacggaaat
      541 catcaaggcc gggtccggct cttaaaccag tatattgaag gataatcgct atgcgtctcc
      601 ttatcgtcgg cggactgcag ggccaaatcg gcctggccac caaaatcgcc atggacaagg
      661 gtgccaaggt cacccatgcc gcggacatag aacaggcttt ggccaccctt cgcggcggca
      721 tcggcgccga tttggtgttc tgcgatgtcg gcctggacat cggcgccctg gtgcgcggcc
      781 tggccgccga acgcatctgc accccggtgg tggcctgcgg tatcggaacc gacacgcgcc
      841 gcgcggtgga cgcgatccgc gctggcgcca aggaatattt gccgctgccc gctgatccgg
      901 aactgatcgc agcggtgctg gcggcggtgg ccgatgaatc ccatcagctg gtctatgaag
      961 acgaagccat ggccgcggtt ctcagcctgg ccgaccagat cgccggtagc gaagcctcca
     1021 tcctggtaac cggcgaaagc ggtaccggca aggaggtgct ggcccgctat gtccatcgca
     1081 aatcggcacg caacgacaag tcgtttattt ccgtgaactg cgccgccatc ccggaaaacc
     1141 tgctcgaatc ggaattgttc ggccacgaaa agggcgcctt caccggcgcc atcgcccggc
     1201 gcatcggcaa gttcgaggaa gccaatggcg gcaccttgct gctggacgaa atcagcgaga
     1261 tggatgcgag gctgcaggcc aagctgctgc gtgccatcca ggaacgcgag atcgaccggg
     1321 tcggcggcac caagccggtc aaggtcgata tccgcatcat cgccacctcc aaccgcgacc
     1381 tggcggaagc ggtcaagcac ggcaccttcc gcgaagactt gctgtaccgc ctcaacgttg
     1441 tgaatctgcg cattcccgcg ctgcgcgagc gtcccaagga tattcgcgcc ttgtcgcatc
     1501 actttgcgcg caaatatgcc gaagccaatg gcgtgcccta tcgcgccatc tcctccccga
     1561 ccgagcgcct gctgctgggt cattattgga agggcaatgt tcgcgagctg gaaaacacca
     1621 tccatcgcgc cgtgctgctg gcgaccggtc ctgatatctc gcccgaagcc atccgcctgc
//
LOCUS       DASWQN010000717         3001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2180591, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000717 DASWQN010000000
VERSION     DASWQN010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180591"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGC82_12195"
     CDS             <1..192
                     /locus_tag="VGC82_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424072.1"
                     /translation="GHSQTQSDAALGQLDVGGADYHFKHGFGCTDPPTPTWTIKLQLL
                     MCIRVTPPLEGQLDDGAGR"
     gene            273..878
                     /locus_tag="VGC82_12200"
     CDS             273..878
                     /locus_tag="VGC82_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424073.1"
                     /translation="MAYYLVRFYSGSGDRPPEQLIREVVDRELRPKLQQGGGLTRYIT
                     FIADDGRVGSASVYETKAAAQNGLQIAREWADSTGAMQGYQLSQTLEGEIAATIDADI
                     QYEPAFGMARLFPTQATAAQVVETIKKARAGASDTGSRIRTVIVQLPDGRVGSFAAYD
                     SQEKREQHTAAVSRARENPEMRRVLPNDPENIAVRIITSTA"
     gene            1119..2825
                     /locus_tag="VGC82_12205"
     CDS             1119..2825
                     /locus_tag="VGC82_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424074.1"
                     /translation="MITDHSARTDWSRRDMIRGSAAAMMGAYGLTPGYGLAADIPYEY
                     DGSKFQMAAPEPTPKRGGVLRYGILNRPPHFDVHQSGTVGNIGTQGCMFDNLIRRDPR
                     DSGQTIVPDLAHSWEIAQDGKTYTFHLRQGVQFHDGTDFTSADIKATFDRICRPPAGV
                     SIPRTPLFRAVSEINVRDKYTVEFKLSAPRSVNFMMSAFASGWNVIFSKKTLEENGYN
                     LRKIPNIPGTGPYKTQRRVENEIWVMEKNPNYWNKGLPYLDGIEFYNLLPFSPELAAG
                     VLSGRVDYARAVDPGTARRAAATSGMSTVKFYQSVIHATWMNAKRKPFDDPRVRRAMH
                     LIFDKQVLVDVVQDVSPLMVGGFIYPFSEFATPKNQLANRPGYQHDSATAIKEAKALL
                     AGAGQGSNMRTLDFMVRDLNHHKLFGQAMQAMLREVNIQCDLRTVVESVWFGDATAGN
                     YDLAVGAIVSTLLDPSDYFNAWYRTDGPQNYSFWHNAEFDKLVDQIDTEVDADRRLAL
                     IRRAEDAMEQDPPLVPVAWENILDVWFNYVKGHNPKDYFGIYDVVRFDTHWLNKADGI
                     RP"
BASE COUNT          669 a          860 c          883 g          589 t
ORIGIN      
        1 ggacatagcc agacgcagtc ggatgcggca ctgggtcaat tggacgtcgg cggcgcggac
       61 taccatttta aacatggttt cggatgcact gatccaccga cgcctacctg gacaataaaa
      121 ctgcaacttc tgatgtgtat cagggtgacg cctccactgg aggggcagct ggacgacggc
      181 gccggccgat agccagacct aatccttgca tccgcggcgg tgctgccctc taactcgagc
      241 gctgccagaa gaacacaagg agggataata gcatggctta ctatctcgtg cgcttttact
      301 ctggttccgg tgatcggcct cctgagcaac tgatcaggga agttgtcgat cgcgaattgc
      361 gtccaaaatt gcagcaaggc ggtgggctga cccgctacat caccttcatc gcggatgatg
      421 ggagggtcgg gtcggcctct gtttacgaaa ccaaggcagc tgcccagaac ggcttgcaga
      481 tcgcccggga atgggcagac agcacgggtg cgatgcaggg atatcagctt tcccaaacgt
      541 tagaaggcga gatcgctgca accatcgatg ctgacatcca atatgagcca gcgttcggta
      601 tggcaaggtt gttcccaaca caggcgaccg ccgcgcaagt cgtcgagacg atcaagaagg
      661 cccgtgctgg agcgtcggat acaggcagtc gcatccgcac ggtgatcgtg caactgccag
      721 atggccgcgt tggctcgttc gccgcctatg actctcagga aaagcgcgaa cagcacacag
      781 ctgcggtcag cagggctcgg gaaaatccgg agatgaggcg cgtgcttccg aacgatccgg
      841 agaatattgc cgttcggatt atcacctcga cagcgtagtg aatgggcggt gccgcgagtt
      901 ggcagcctcc cggcaccgcc accgcaaact tcagtgctgt aaaattgcca gttgcgaaca
      961 tggttgtaac cgcctggcgt cggtgcctat tctgcctgcg tggcggctga attacctgtt
     1021 catcgggcaa cacaagcgag caggtgacgc catgcgccgt ccaaacaaat acccataaga
     1081 agacgcttag taaaaagcca aacgggagga ttccgacaat gattaccgac cactccgcgc
     1141 gtactgattg gtcgcgccga gatatgattc gcggcagcgc tgcggcgatg atgggggctt
     1201 acggtctgac cccgggctac ggcctcgccg ctgatattcc gtacgagtac gacggatcga
     1261 aatttcaaat ggctgcaccg gagcccactc ccaagcgagg cggggtactc cgctatggca
     1321 tcctcaatcg gccgccgcac tttgacgtgc atcaatcggg cactgtcggc aacatcggca
     1381 cgcagggctg catgttcgac aacctgatcc ggcgcgatcc gcgcgacagc ggccagacta
     1441 tcgtccccga tctggcgcac agctgggaaa tcgcgcagga cggtaagacc tacacgttcc
     1501 acctgcgcca gggcgtgcag ttccacgatg gaacggattt cacatctgct gacataaagg
     1561 cgacctttga ccgtatttgc aggccgccgg ccggcgtcag cattccgcgc accccgctgt
     1621 ttagagcggt cagcgagatc aacgtgcggg acaagtacac cgttgaattc aagctgtcgg
     1681 cgccgcgctc ggtcaatttc atgatgtccg ccttcgcaag tggctggaac gtcatattca
     1741 gcaagaagac gctggaagag aatggttaca atctgcgcaa gatccccaat atccctggta
     1801 caggccccta caagacgcaa cggcgggtcg agaacgagat ctgggtgatg gagaaaaacc
     1861 cgaactactg gaacaagggc ctgccttacc tcgatggcat cgaattctac aatctgctgc
     1921 cgttctcccc ggagctggcg gccggcgttc tgtcaggccg ggtagattac gcccgcgcag
     1981 tcgacccagg caccgcacgc agggcggccg cgacctcggg catgtccacc gtaaagttct
     2041 atcagagcgt gattcacgca acttggatga atgccaaaag gaaaccgttc gatgatccgc
     2101 gtgtcaggcg cgccatgcat ctgatcttcg acaaacaggt cctggtggac gtcgtacagg
     2161 atgtctcccc gctgatggta gggggcttta tctacccgtt ttctgagttt gccacgccca
     2221 agaaccaact cgccaatcgg cccggttatc agcacgactc cgccactgcg atcaaagagg
     2281 ctaaagcctt gttggccggc gcgggccagg gcagcaacat gcgaacacta gatttcatgg
     2341 tgcgggacct caaccatcac aagctctttg gccaagccat gcaggcgatg ctgcgggagg
     2401 tgaatattca atgtgacctg cgaacggtgg tcgaatcggt gtggttcggt gacgcgacgg
     2461 ccggcaatta cgatctagcc gttggcgcca tagtctcgac cttgctcgat ccttccgact
     2521 actttaacgc gtggtatcgc accgacgggc cgcagaacta ctcgttttgg cacaacgccg
     2581 aattcgacaa actggtggat caaattgata ccgaagtcga cgccgacagg cgcttggcgc
     2641 tgatccggcg agccgaggac gccatggagc aggatcctcc gctggttccg gtcgcctggg
     2701 aaaacatcct tgatgtctgg tttaactacg tcaaaggcca caatcccaaa gattatttcg
     2761 gtatctacga cgttgtccgg ttcgatacac actggctgaa taaggccgac ggcataagac
     2821 cctgaccaat tgggctcggc gggcgatcgc gcaaacaaaa cgctggctgc cagattgccc
     2881 ggtgcttcag atgcctgagg tcactgatgg cggtggaagc gtggcattga acgtggtcgc
     2941 tggccaagca aggcctttgc cggcatcgtt tgttgctcgc ctggcaggga tcgagttgaa
     3001 t
//
LOCUS       DASWQN010000718         5378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_201251, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000718 DASWQN010000000
VERSION     DASWQN010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201251"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VGC82_12210"
     CDS             complement(<1..598)
                     /locus_tag="VGC82_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0424075.1"
                     /translation="MDFDLFVIGGGSAGVRCARISAGHRALVGIAEERHWGGTCVNIG
                     CVPKKLLVQAGEYGGWADDAAGFGWTITKGSHDWGKLIAAKDREITRLEGIYRKMLAS
                     AGATVFDGRAVLVDPHTLEVAGKRVTAENIVIATGGYPERPSIPGAELGIISDDAFYL
                     KTMPRHVVMVGSGYIAVEFAGIFRNLGAEVHLVYRQPLP"
     gene            complement(882..1496)
                     /locus_tag="VGC82_12215"
     CDS             complement(882..1496)
                     /locus_tag="VGC82_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424076.1"
                     /translation="MMMAAGAQEDGGVVKTSHEPAQPSKDRENRDDRGKHFLEAGQRK
                     RLTNLDSLAVPEQLIAFVEAQPLKILDAQRRDVGRPIGLCTLSHGRVPVGLVFPVKPL
                     LKLQNPPTEVLDDLGQLSQRLLHRLDHLDRRDDFAYRRTRHVRRSVEADAQPERRAPR
                     LLFGVQLASRLPYGRPWWPTSAAVWRQSVSDRPLQPPFSARRPS"
     gene            complement(1716..2498)
                     /locus_tag="VGC82_12220"
     CDS             complement(1716..2498)
                     /locus_tag="VGC82_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0424077.1"
                     /translation="MDLGLQGKKAIVCAASKGLGRACAISLAREGVELVITARTASTL
                     EETAAEIRQMTGAKVTAVAGDITSEAGRAAALAACPAPDILVNNNGGPPRGDFREWDR
                     DMWIKAVDANMLAPIFLIKSVIDGMIDRKFGRIVNITSGSVKSPIPELGMSNGARAGL
                     TGFVAGLARQVARHNVVINNILPGPFLTDRLRGGFQEAARKTNRPVDELVQERQANSP
                     AGRAGDPAEFGDACAFLCSAKIGFIVGQNLLLDGGAFNSSST"
     gene            complement(2616..3413)
                     /locus_tag="VGC82_12225"
     CDS             complement(2616..3413)
                     /locus_tag="VGC82_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0424078.1"
                     /translation="MTGRDGGADLQPIGNSPVVILVRPQLADNIGACARAMANGGLFD
                     LRLVAPRDGWPQEKAWRMASGADRILDAATVHGTVADAVADLHHVFATCPRPRHIVKP
                     VLTARGAAAELREICTRGLRAGLLFGPERAGLDNDDMAQADALVRFPLNPAFMSLNLA
                     QAVMVMAYEWWSEEDATPSRTLMTNETQVATKGRLDNFLVHLVDQLDACGFLRNLPKR
                     AGMVRNIRHFFQRGEVTEQELRTLHGVVTELAIGRRQRGRLETDQAE"
     gene            complement(3413..4762)
                     /gene="cysS"
                     /locus_tag="VGC82_12230"
     CDS             complement(3413..4762)
                     /gene="cysS"
                     /locus_tag="VGC82_12230"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY0424079.1"
                     /translation="MPELFLHNSLTRRKERFEPADPAHVRMYVCGLTVYDLAHIGNGR
                     AIVVYDVFARLLRLLYPSVTYVRNITDVEDKINARAREIGEPISVLTARTTADFHADM
                     AALGALPPDQEPRATAHIAEMIMIIERLIASGHAYVAEGHVLFAVASDPNYGALSGRS
                     PEDLLAGARIDVAPYKRDPGDFVLWKPSTDDLPGWSSPWGRGRPGWHIECSAMSWRYL
                     GETFDIHGGGSDLIFPHHENEMAQSLCAFPGSRFARYWIHNGMLQLNGEKMSKSLGNF
                     FTVRDILAKAPGEAIRLLLLRTHYRAELDFSDQGLAEAKRELDRFYRALEQAPGAGAG
                     AVPAAVLEALCDDLNTPLAISTMHGLADAAMTGNREAASGLRAAGAVLGLLQQDPDAW
                     FRGGADDSAEIDAAIAERLAARKSRDFARADAIRADLAAKGIVLEDGPAGTTWRRNR"
     gene            4897..>5378
                     /locus_tag="VGC82_12235"
     CDS             4897..>5378
                     /locus_tag="VGC82_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY0424080.1"
                     /translation="MDPTLRTASRLRRFLAVFGPGLVVMLADTDVGSVITAGQSGVQW
                     GYRLLLLQLVLVPILFMVQELTVRLAIFTGRGHGELIRDTFGPAWAWVSAVGLGVASI
                     GALLTEFSGVAGVGELYGLSRYASLPVAAAALLLVVLTGSYRRVERLAMAVGLFELAF
                     "
BASE COUNT          943 a         1787 c         1662 g          986 t
ORIGIN      
        1 acggcagcgg ttggcgataa accagatgca cttccgcacc caggttgcgg aatatcccgg
       61 cgaactcaac cgcgatgtag cccgacccga ccatgacgac gtgccgcggc atcgtcttca
      121 gatagaaggc atcgtccgag atgataccga gttcggcacc gggaatactg ggacgctccg
      181 gatatccgcc ggtggcaatc acgatatttt ccgcggtcac gcgcttgccg gcgacttcca
      241 acgtgtgcgg gtccacgagg acagccctgc cgtcgaacac ggtcgcaccg gcactggcca
      301 gcatcttgcg gtaaattccc tccagccggg tgatctcccg gtcttttgcg gcaatcagct
      361 tgccccagtc gtgggacccc ttcgtaatgg tccagccaaa accggcggca tcatcggccc
      421 agccgccata ctctcccgcc tgcaccagca attttttcgg cacacagcca atattcacgc
      481 aggtgccgcc ccagtggcgt tcctcggcaa tgccgactag agcccggtgg ccggcggaga
      541 tgcgagcgca gcgaaccccg gcagagccgc cgccgatgac aaacaggtcg aagtccatgc
      601 gcgtacgtcc tcctcatcgc gggttgctaa tgtgtgacct gaggaacgat ggcgcaagat
      661 tggccgcgcc aggcccagca ctacccgcca tccgcgaaac gtcgggcgat cgccggatta
      721 gtccgtggaa cgtgatccgg cagttcccat atagttttga ggccacgcgt ttccgcgcta
      781 tgatggccgt cacgaggtaa tggaaatggc tgtgcgccga ccgatgtcca cgaccaacac
      841 tcaggccgag cccgccagca aggcggctcg caccacgtct atcaagatgg ccgccgcgcc
      901 gaaaagggcg gttgcaaagg ccggtccgaa acggactgcc gccaaaccgc cgcgctcgtc
      961 ggccaccacg gacggccgta aggcagccgc gatgctaact gaactccgaa cagaaggcgc
     1021 ggcgctctcc gctcgggttg ggcgtctgct tcaacgcttc tcctgacatg gcgcgtacgc
     1081 cgataagcga aatcgtcgcg acggtcgagg tggtcaagac gatgcagcag acgctggctc
     1141 agttgaccca ggtcgtcgag aacctcggtg ggcgggtttt gaagcttgag cagcggcttg
     1201 acgggaaaga ctaagcctac cggcacgcgg ccatgagaga gcgtgcaaag gccgattggc
     1261 cggccgacgt cacgccgctg ggcgtcgagg atcttgagcg gctgggcctc aacaaaagca
     1321 atcaactgtt ctgggacggc aagcgaatcg aggttcgtca atctcttacg ctgaccggct
     1381 tccagaaagt gcttgccgcg gtcgtctcgg ttttcgcgat ccttggaggg ctgggcgggt
     1441 tcatgactgg tcttaacaac gcctccatct tcctgtgcgc ccgcggccat catcaacttg
     1501 gatgtccggc ttcgacgttt cccctggcgc ccatgccggc ccaacctgcc gcatccgtga
     1561 agccatgatg actggcaatg ccccggactg gggatatttc ggactggcaa tatttcggac
     1621 gagcaatgct tcggcgggaa tattggtaac aaggaaaatg tgcggacaac cccgcatttt
     1681 ttcttgctcc ggcccgacct gtcagaccct ccacctcagg tgctggacga attgaacgcc
     1741 ccgccatcca gcaacaaatt ctgcccgaca ataaagccga ttttcgccga acacaggaac
     1801 gcgcacgcgt cgccgaactc cgccggatcg ccggcgcgcc ccgccgggct gttcgcctgc
     1861 cgctcctgca ccagttcgtc caccgggcga ttggtcttgc gggcagcctc ctggaacccg
     1921 ccgcgcagcc ggtcggtcag gaacgggcca ggcaggatgt tgttgatcac cacattatgc
     1981 ctcgccacct gccgcgcgag gccggcgaca aagccggtca gcccggcgcg ggcgccattg
     2041 ctcatcccca gctcagggat cggcgacttt accgatccgg acgtgatgtt gacgatgcgg
     2101 ccgaacttgc ggtcgatcat accatcgatc accgacttga tcaggaagat cggcgccagc
     2161 atgttggcat cgaccgcctt gatccacata tcgcgatccc actcgcgaaa atcgccgcgc
     2221 ggtggcccgc cgttattgtt caccagaata tccggcgccg gacatgccgc gagtgccgcc
     2281 gctcggcctg cctcactcgt gatatcccca gcgacggcgg tgaccttcgc gcccgtcatc
     2341 tggcggattt ccgccgcggt ttcttccagc gtcgaggcgg tgcgggcggt aatcaccaat
     2401 tccacgccct cccgcgccaa cgaaatcgcg caggctcggc cgagcccctt gctggcagcg
     2461 cagacgatgg cttttttgcc ttgcagtccc aggtccatga tcgttcctcc gtttggccgc
     2521 gatgtaagcc ggccgcatct ggcggaactc tatcaggggg aaacgggatc gcctactgac
     2581 ccggccgcgc aagacggtgg ctgtcgcggt tgaggttatt cggcctgatc agtctccagc
     2641 cgtccgcgct ggcgccggcc gatcgccagt tccgtcacca cgccatgcag cgtgcgcagt
     2701 tcctgttcgg tgacctcgcc gcgctggaag aaatgccgga tgttgcgcac catgcccgcc
     2761 cgtttgggca aattgcgcag gaagccgcac gcatcgagct gatccaccag atggacgagg
     2821 aagttgtcca gcctgccctt ggtcgccacc tgcgtctcgt tggtcatcag cgtgcgggac
     2881 ggggtggcgt cctcttcgct ccaccactcg tacgccatca ccatgaccgc ctgcgccagg
     2941 ttcagcgaca tgaacgcggg gttgagcggg aagcgcacca gcgcgtcggc ttgcgccatg
     3001 tcgtcattgt ccaggcccgc tcgttcggga ccaaacagca ggccggcgcg caggccgcgc
     3061 gtgcagattt cacgtaattc cgccgccgca cccctggccg tcaataccgg cttgacgatg
     3121 tgccgaggcc ggggacaggt ggcaaagacg tggtgcaagt cggcgaccgc atcggcaacc
     3181 gtcccatgca ccgtcgccgc atccaggatg cgatcggcac ccgaggccat gcgccaggcc
     3241 ttttcctgtg gccatccgtc cctgggggca acgaggcgca ggtcgaacaa gccgccattc
     3301 gccatggctc gcgcgcacgc gccgatatta tcggccaact gcggccgcac caggatcacc
     3361 accgggctgt tgccgatcgg ctgcaggtca gcgccgccat cgcgtccggt cattacctgt
     3421 tgcgccgcca ggtcgtgccc gccggtccgt cctccagcac gattcccttg gcggccagat
     3481 ctgcccggat tgcgtcggca cgggcaaagt cgcgcgattt ccgagccgcc agccgttcgg
     3541 cgatcgcggc gtcgatttcc gccgagtcgt ccgctccgcc gcggaaccag gcgtcggggt
     3601 cctgctgcaa caagcccaat acggcgccgg ccgctcgcag cccggatgcg gcctcgcggt
     3661 ttccggtcat cgccgcatcc gccagcccat gcatcgtcga gatcgccagc ggcgtattga
     3721 ggtcgtcgca cagcgcctcc agcaccgccg ccgggacagc gcccgcgccg gcaccgggag
     3781 cctgttccaa agctcggtag aaacgatcca gctcgcgttt cgcctcggcc aggccctgat
     3841 ccgaaaagtc cagttccgca cggtaatgcg tccgcagcaa cagcaagcgg atcgcttcgc
     3901 ccggtgcctt cgccaggatg tcgcggacgg tgaagaaatt gcccaggctc ttggacatct
     3961 tctcgccgtt caactgcaac atgccgttgt gtatccagta acgggcgaac cggctgccgg
     4021 ggaaggcgca caggctttgc gccatttcat tctcgtggtg tggaaagatc aggtcgctac
     4081 cgccgccgtg gatgtcgaac gtttcgccca ggtagcgcca ggacatggca gagcactcga
     4141 tatgccaccc gggtcgtccg cgtccccagg ggctgctcca gccgggcaaa tcgtcggttg
     4201 acggcttcca aaggacaaaa tcgcccggat cgcgcttgta aggtgcgacg tcgatacggg
     4261 cgccggcgag caggtcttcc gggctgcggc ccgatagcgc gccgtagttc ggatcgctcg
     4321 cgacggcgaa caggacatga ccctccgcca cgtaggcatg gccggatgca atcagccttt
     4381 cgatgatcat gatcatctcg gcgatgtggg ccgtggcgcg cggctcctgg tccggcggca
     4441 acgcgcccaa ggcggccatg tcggcgtgaa aatccgccgt ggtgcgggcg gtcaggacgc
     4501 tgatcggctc tccgatctcg cgcgcccggg cattgatctt gtcttctacg tccgtgatgt
     4561 tgcgaacata ggtcaccgac ggatacagaa gtcgcaacaa gcgggcgaac acatcataaa
     4621 cgacgatcgc ccgcccgttg ccgatatgcg cgaggtcgta caccgtcagg ccgcagacat
     4681 acatgcgcac atgagccgga tcggccggtt cgaaccgctc cttgcggcgg gtcaggctgt
     4741 tgtgcaggaa aagttcgggc atcatacgga tccattgtcg gctggggctt tgtcccgcaa
     4801 acatggtgtc ggcgcaatgg catttgacac cggcgggaag ggacgttagc gacgccctcc
     4861 gctcaccgag agccattcgc aaatcccggt gcatccttgg atcccaccct ccgcacggca
     4921 tcccgcctgc gccgcttcct ggctgtgttc ggccccggcc tcgtggtgat gttggcggat
     4981 accgacgtcg gcagcgtcat caccgccgga caaagcggcg tgcagtgggg ctaccgcctg
     5041 ctgctgctgc aactggttct ggtgccaatt ctcttcatgg tgcaggaact cacggtgcgc
     5101 ctcgcgatct tcaccggaag gggccacggc gagttgattc gcgacacatt cggcccagcc
     5161 tgggcctggg tgtcggccgt cggcctcggc gttgcctcga tcggcgcatt gctgacggaa
     5221 ttctccggtg tcgccggcgt cggcgagctg tacggactgt cgcggtatgc gtcgttgccg
     5281 gtggcggccg cggccctgct gctggtggtt cttactggct cataccgacg tgtcgaacgg
     5341 ctcgcgatgg ccgtgggtct gttcgaactg gcattttt
//
LOCUS       DASWQN010000719         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_992771, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000719 DASWQN010000000
VERSION     DASWQN010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992771"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="VGC82_12240"
     CDS             complement(<1..176)
                     /locus_tag="VGC82_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY0424081.1"
                     /translation="MNAVDDAITSRRSIRRFLPTPVPVATVEAILDVAARAPSGTNMQ
                     PWRGYVVAGAARDAL"
     gene            complement(173..1207)
                     /locus_tag="VGC82_12245"
     CDS             complement(173..1207)
                     /locus_tag="VGC82_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0424082.1"
                     /translation="MSDVTHDSFSFTNLPLLQGGTLPEVTLAYVTRGRLAPDGRNAIL
                     VTHGYTSGPRMIEPGIASSEGAWSTLIGPGAPIDTDRYYVVCSNMLGSSYGSTNAASA
                     DPRTGKPYGSKFPKITMTDIVTAQKRLLEHLGVTHLRAVIGPSFGGFQAFQWAVTFPD
                     FMDGVAPVVTSPLPPQSDRVEGLRQWFEKDPNWNGGDYYHTGGVHGTLTALRVDTLRR
                     YGLAASLAERFPNPADCDAELRRVASAWADVFDANSLFILGGAMAAYDITKDYGRIKV
                     PVLYVLSRTDALFPPSLAPGVMADLRAAGVDATYVEIDSEHGHLASGADAAKWAPALR
                     AFIARLEAAE"
     gene            1337..>1630
                     /locus_tag="VGC82_12250"
     CDS             1337..>1630
                     /locus_tag="VGC82_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEY0424083.1"
                     /translation="MTVIALATALPAAAANVTVAMHRLTQQGTGEDLGKIVISGSDAG
                     AVFTLDLHGLPPGQHGFHVHENSGCGPTNMNDVRIPGGAAGGHLDPDHTNKHEG"
BASE COUNT          307 a          528 c          505 g          290 t
ORIGIN      
        1 agcgcatccc gtgcggcgcc agccacgaca taacctcgcc aaggctgcat gttcgtgccg
       61 ctgggtgccc tcgcggcgac atcaaggatc gcctccaccg tcgcgaccgg caccggtgtc
      121 ggcaggaatc gccggatgga gcggcgggag gtgatcgcgt cgtcaacagc gttcattcag
      181 cagcttccag tctggcgatg aaggcgcgaa gcgccggcgc ccatttggcg gcatccgcgc
      241 cagatgccaa gtgaccgtgc tcgctgtcga tttccacata cgtggcatcg accccggccg
      301 cccgcagatc cgccatgacc cccggcgcca gactgggagg gaacagcgcg tcggtccgcg
      361 acagcacata aagcaccggg accttgatcc gcccatagtc cttggtgatg tcataggccg
      421 ccatcgcacc tcccaggatg aacagggagt tcgcatcgaa cacatccgcc caggccgacg
      481 cgacccgccg caattccgcg tcgcaatccg ccggattggg aaaccgttcg gcaaggctgg
      541 cggcgagccc ataccgcctg agcgtgtcca cgcgcagcgc tgtcagcgtc ccatgtacgc
      601 cgccggtgtg gtagtagtcg ccgccgttcc agttcgggtc cttctcgaac cattgccgca
      661 gcccctccac ccggtcggat tgcggcggca aaggcgacgt cacgacaggg gccaccccat
      721 ccatgaaatc cggaaacgtc accgcccatt gaaacgcctg aaatccgccg aacgacggtc
      781 cgatcaccgc tcgtagatgg gtgacgccca agtgttccag aagccgcttc tgagcggtga
      841 cgatgtccgt catggtgatt ttcgggaatt tcgacccata cggtttaccg gtgcgcgggt
      901 cggctgacgc cgcgttggtg gagccgtaac tcgaccccag catgttggag cacacgacgt
      961 agtagcggtc ggtgtcgatc ggcgctccgg gaccgatcag cgtgctccac gctccttccg
     1021 acgacgcgat gccgggttcg atcatccgcg gaccgctggt atagccgtgg gtaaccagga
     1081 tcgcgttgcg gccatccggc gccaaacggc cgcgcgtgac atacgccagc gtcacctccg
     1141 gcagcgtccc accctgaagc agcggcaggt tggtgaacga gaaactgtcg tgggtgacat
     1201 cggacatgat ggattcccta tggatccccc tgatggatac ccctgggggg attcggatca
     1261 ggttgccatc gaccgcgatt gcatgcgaat caacggcgca tagcggcaag gggacactat
     1321 gaaatatacg ctttgcttga cggtcatcgc gcttgccacc gctctgcccg cggcagcagc
     1381 gaatgtgacg gtcgctatgc accggctgac gcagcaagga accggcgagg atctcggcaa
     1441 gattgtcatt tctggttcgg atgccggtgc cgttttcacg ctcgatctgc acggtttgcc
     1501 gcctgggcag catggtttcc atgtgcatga aaacagcggt tgcggcccga ccaatatgaa
     1561 tgatgtccgc attccgggcg gcgcggctgg tggccatctt gatccggacc acaccaacaa
     1621 gcacgagggt
//
LOCUS       DASWQN010000720         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_69863, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000720 DASWQN010000000
VERSION     DASWQN010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69863"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /gene="glgA"
                     /locus_tag="VGC82_12255"
     CDS             <1..1202
                     /gene="glgA"
                     /locus_tag="VGC82_12255"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001901.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEY0424084.1"
                     /translation="TQDGLPVYVLLCPQLYDRPGNPYGDASGRDWPDNDVRFGRFASA
                     AAQLAAGTLDKNWAADLVHANDWQAALVPAYLAWNAVRIPSILTIHNLAYQGLFPKES
                     LRRIGAPADTFHIDGLEFYDKLSFLKGGLVYASHLTTVSETYAREITTAELGCGLEGL
                     LRRRSDAAQLTGILNGIDESWDPRLCSQLAQPFAAGDWEGKQANADYVRQQFGLALSR
                     GPIFGLVARLVHQKGVDLVLSAADEIIEAGGQIIVTGSGEPEIEQALVEAHRRRPDAI
                     GVVIGFNDGQARRIFAGSDFTLMPSRFEPCGLSQMYAQRFGSLPIGHQTGGLAETITD
                     GETGFLFDQPSTESFLGGVRRAFATFRAKDRLNSMRQSAMSRSFSWDISAACYSALYR
                     KALSSQA"
     gene            complement(1172..2338)
                     /locus_tag="VGC82_12260"
     CDS             complement(1172..2338)
                     /locus_tag="VGC82_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424085.1"
                     /translation="MQTGFGPFVAVYLTTQKWTQVEIGLVLSIGGVVGLIGQMPGGAI
                     IDAARSERLVAGLAIAAIGTSALAYAAWPIFPVVAAAATLHAAASCVLGPAIAAISLG
                     LVGPLAISERLGRNARYASLGNGVAAGVMGTAGYLLSSRSVFLVTFVLAIPTLLALAR
                     IRESEIDVARAHGAVPRDTADVTATSVASLVRRRPLLVFAASLLLLQLANAAMLPLMA
                     GVVTTRSSQWAPVLIAACIIVPQAIVALMSPSVGRMAQQWGRRPLLLVGFAALVVRGL
                     LFATVRDPYLLVAVQVFDGITAAVFSVMVPLIVADVAFGSGHFSLAQGIVGTAVGIGA
                     SLSNVLAGYASDQFGSTVAFTGLACVAAAGLAMIWLVMPETRRLNQAWEDKALR"
BASE COUNT          426 a          816 c          759 g          351 t
ORIGIN      
        1 cgacccagga cggcctgccc gtctacgttc tgttgtgccc gcagctttac gaccgccccg
       61 gcaaccccta tggcgatgcc tcgggccggg actggccgga caatgatgtc cgcttcgggc
      121 ggtttgcctc ggccgcggcc cagctcgccg cgggcactct ggacaaaaat tgggcagctg
      181 acctggttca tgccaacgac tggcaggccg ctctcgtgcc ggcctacctg gcctggaacg
      241 cggtcaggat ccccagcatt ctgaccatcc acaacctggc ctatcagggc ttgtttccaa
      301 aagagtcgct acgccggatc ggcgcccccg cggacacctt ccatatcgac ggcctggagt
      361 tctacgacaa gctgtccttc ctcaagggcg gcctcgtcta cgcctcgcat ctgacgaccg
      421 tcagcgagac ctatgccagg gagatcacca cggcagaact cggctgcggg ctcgagggcc
      481 tactgcgccg ccgctccgac gccgcgcaac tgacgggcat attgaacggc atcgacgaaa
      541 gctgggatcc acggctctgc tcgcaactgg cgcagccgtt tgccgccggg gactgggagg
      601 gcaagcaggc gaatgccgac tatgtccgcc agcagttcgg cctggcgctg tcgcgcggtc
      661 cgatcttcgg tctcgtcgcc cgtctggttc atcagaaagg cgtcgatctc gtgctctcgg
      721 ccgcggacga aatcatcgag gccggtggcc agatcatcgt gaccggcagc ggcgagcccg
      781 agatcgagca ggcgctggtc gaggcgcacc gccgcagacc ggacgcgatc ggagtcgtga
      841 tcgggttcaa cgacgggcag gcgcggcgga tatttgccgg cagcgatttc acgctgatgc
      901 cgtcgcgctt cgagccatgc gggctgagcc agatgtacgc gcagagattc gggtcgctgc
      961 cgatcggcca ccagaccggc ggcctcgcgg aaaccatcac cgacggggaa accggattct
     1021 tgttcgatca gccgtcgacc gagtcgtttc tcggcggggt gcggcgcgcc ttcgcaacct
     1081 tcagggccaa ggaccggctc aactcgatgc gccagagcgc gatgtcgcga tccttcagct
     1141 gggatatttc ggcggcgtgc tacagcgcgc tttaccgcaa ggccttgtcc tcccaggctt
     1201 gattcagccg ccgggtttcc ggcatcacca accagatcat ggccagcccc gccgcggcga
     1261 cgcaggcgag ccccgtaaag gcgacggtgc ttccgaactg gtcgctggcg tagccggcca
     1321 gcacgttgct gagcgatgcg ccgataccga cggctgtgcc gacgatcccc tgcgcgagac
     1381 tgaagtggcc gcttccgaac gcgacgtcgg cgacgatcag cggcaccatg acgctgaaga
     1441 ccgccgcggt gatgccgtcg aacacctgca ccgccaccag caaataggga tcgcgcacgg
     1501 tggcaaacaa cagcccgcgg acaaccagag cggcaaagcc gacaagcagc agcggccgcc
     1561 gtccccattg ctgcgccatc cttccgaccg acggcgacat cagcgcgacg atcgcttgcg
     1621 gaacgataat gcaggcggcg atcagcaccg gcgcccactg gcttgaccgg gtggtcacga
     1681 cccccgccat cagcggaagc attgcggcgt tggcgagttg cagcagcagc aggctcgctg
     1741 cgaagaccag taacggtcga cggcgcacca gactggcaac gctggtggcg gtgacatcgg
     1801 ccgtgtcgcg gggcaccgcg ccgtgcgccc gcgccacatc gatctcgctc tcccggatgc
     1861 gggccagcgc cagcaaggtc gggatagcca ggacgaaggt gaccagaaac accgaacggc
     1921 tcgacagcag gtagccggcc gtccccatca cgcccgcggc gacgccgttt ccgagcgacg
     1981 cgtagcgggc gttgcgcccg agccgttcgc tgatcgccag cggcccgacc agaccaaggc
     2041 tgatcgccgc aatcgccgga ccgagcacgc agctggcggc ggcgtgcagg gtcgcagcgg
     2101 ccgctacgac cggaaagatt ggccacgccg cgtaggccag ggcgctggtc ccgatggcag
     2161 cgatcgcaag cccggcgacc agccgttccg agcgggccgc atcgatgatg gctccccccg
     2221 gcatctgacc gatcagaccg accacgccgc cgatcgacag caccaggccg atctccacct
     2281 gggtccattt ctgcgttgtc agataaaccg cgacgaacgg cccgaacccg gtctgcacat
     2341 cggccagaaa ga
//
LOCUS       DASWQN010000721         5449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2577300, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000721 DASWQN010000000
VERSION     DASWQN010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2577300"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1350)
                     /locus_tag="VGC82_12265"
     CDS             complement(73..1350)
                     /locus_tag="VGC82_12265"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424086.1"
                     /translation="MKEAPENLPPGSYPALRPGDTDWQRTLWAMVGIQFVMTAAVNFL
                     SPIIPLMLPQLGVETPQGIAIWAGLITGSTSFVAAFASPVWGRIADKHGRKLSLLRSS
                     FAIAVFTALMGLSVNVWQFFGARAVMGVFAGFSSAAIALVASQVPERRLGYALGWLSS
                     GQLVGSLVGPVVGGLLADATGSYRIPFFLCAGITCAGLVLVWAVVTENFTAPKQAKAK
                     SLFSGIGLLTGSASLSALFMVLLMAQLSTRTIAPVVTIFVQELIGSPPQIATLAGVAF
                     SITGLANLMAAPFLGNRSDIIGYRKVLLISLLGATLTSAPQIFVSDYWTFVAERFSVG
                     LFIGGILPTANALIGRSVSRENRGTIYGLTASATFLGNSIGPLMGGGIAAGLGIRWVF
                     AVTTVLLAANLAWVWFTVPELLDEQEDYQPPDQ"
     gene            1478..2230
                     /locus_tag="VGC82_12270"
     CDS             1478..2230
                     /locus_tag="VGC82_12270"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY0424087.1"
                     /translation="MSSRQDRKRIGLLLPSSNTTQEPEFTRIMPYGVTLHVARLPLRT
                     VEPASTARIVEDIETESQKLADADVDAIVLAATAPSSRNGIGYDQELIKRIETASGKP
                     ATTASTALIQALKTLDATHLVVGAPWSEATNVTTAAFVQAHGFTVLAHQAMGHLSNLE
                     IGDLEEQTAYEMGIAIDRPEARTVMLACGNWPTLGIVDRLESALGKPVLTTNQVSLWA
                     VLRLVGYHAPVFGWGLLLREHMIEPASVVLAL"
     gene            2227..3186
                     /locus_tag="VGC82_12275"
     CDS             2227..3186
                     /locus_tag="VGC82_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEY0424088.1"
                     /translation="MKIVIPDDYQDIVDRLSCFSLLVGHDVIRYREPAADLDQLVERL
                     HPAEVVVAIRERVNFSRALLQRLPNLKLIALVGRAATTIDYGACKDCGVLVSTGASNS
                     PVAPAELTVALIVASRRNIALEAERMRRGDWPCTLSHRLAGSTLGIFGLGSIGSLVAR
                     AGAGMGMNVLAWGQQASAAKAQAAGYDFAAGKADLFERSDVLSLHVRLRPDTIGIVGP
                     EDLARMKPTALLVNSSRAELIKRGALLAALEAGHPGYAAVDVYEEEPITGGNHPFLSM
                     PNVLCTPHLGWAEWDNFELYFRECFEQIVAYFGGAAMRLAGLG"
     gene            complement(3204..3785)
                     /locus_tag="VGC82_12280"
     CDS             complement(3204..3785)
                     /locus_tag="VGC82_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY0424089.1"
                     /translation="MTGIHSLAVFCGSRVGRKPDYAAAGRALGLGLAQADIRLVFGGG
                     RIGIMGIVADAVLEAGGKVLGVIPEFLTQWEVAHEYVTEMVITDSMHTRKRRLYEESD
                     AFLIMPGGLGTFDEAFEIITWRQLRLHDKPIMLCNVAGSAAPLVSVIDHAIEQGFADP
                     ACRGLFEVIEGVPAVLERLRTLPAGTRGQAERL"
     gene            3891..4361
                     /locus_tag="VGC82_12285"
     CDS             3891..4361
                     /locus_tag="VGC82_12285"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0424090.1"
                     /translation="MGRLYRMTKRIAIYSAIMLAAVGISSIGVALADGLNIIPIRQAG
                     MSLAGGDFAFIRSTVAAKGDVKALEAPAKAIAKWAAVIPSVFPKGTETGNDTKALPEV
                     WADNAGFQKIALALGEASTKLAAAAKAGDADEVAADAKLVGDQCGACHKTYRAK"
     gene            4711..>5449
                     /locus_tag="VGC82_12290"
     CDS             4711..>5449
                     /locus_tag="VGC82_12290"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424091.1"
                     /translation="MRLSIRHFCLALVALSIAITARSASAEDTIKVGYVDPFSGPFAS
                     GGDEFLKVFAFILEKANAKGGALGKKFELVPFDDKLQPAEALIALKNITDQNIPFVMQ
                     CTGSNVGAALVDGVSKHNARNPDNRVLYLNCGALATELTNEKCDFWQFRFAGNVEMRA
                     AARIKALPKDVKKVYLLNQDYLFGQSVQHDTRKWLAKLRPDIEVVGDELMPFGKVQDF
                     SSYVAKIKASGAQSVITGNYNRDLNLLI"
BASE COUNT         1092 a         1703 c         1649 g         1005 t
ORIGIN      
        1 cggattcaac cactccgagc aaatctaagc ggtgctaacg ccgcacgaat tccatgggca
       61 acgtttagaa agttactgat caggaggctg ataatcctcc tgttcatcca gcaactccgg
      121 caccgtaaac caaacccatg ccaaattggc cgccagcagc accgtcgtca ccgcgaatac
      181 ccagcggatg ccgagccccg ccgcgatccc gccccccatc aatggtccga tcgagttgcc
      241 gaggaacgtc gccgaagcag tcaaaccata gatggtgccg cggttctccc gcgacacgct
      301 gcgccctatc aaggcgttcg ccgtgggaag gatgccgccg ataaacaggc ccaccgagaa
      361 tcgttcggcg acgaacgtcc agtagtcgct gacaaagatc tgcggcgcgc tggtcagcgt
      421 ggcgcccagc aggctgatca gcagcacctt gcgatagccg atgatgtcgc tgcggttgcc
      481 caggaacggc gcggccatca ggttcgccag cccggtgatg gagaacgcga cccccgccag
      541 cgtggcgatc tgcggcggcg aaccgatcag ctcctgcacg aagatggtga cgaccggcgc
      601 gatggttctt gtgctgagct gcgccatcaa cagcaccatg aacagcgcgc tcaggctggc
      661 cgaaccggtc agcaggccaa tcccggagaa tagcgacttc gccttggcct gcttcggcgc
      721 ggtgaagttc tcggtgacca ccgcccacac caggaccagc ccggcgcaag taatgccggc
      781 gcacaagaag aacggaattc gataacttcc ggtcgcatcc gccagcaggc caccgaccac
      841 cggaccaacc agcgagccga ccaactgccc ggacgaaagc cagcccagcg catagcccag
      901 ccgccgctcc ggcacctggc tggcgaccag tgcgatcgca gcggaggaaa atccggcgaa
      961 cacccccatc acggcgcgcg cgccgaagaa ctgccacacg ttgaccgaca gccccatcag
     1021 cgcggtaaac accgcgatcg cgaagctgga acgcaaaagc gacagcttgc gtccgtgctt
     1081 gtccgcgata cggccccaga ccggtgaggc gaaggcggcg acgaacgacg tggagccggt
     1141 gataagtccg gcccagatag cgattccctg aggcgtctcg acgccaagct gcggcaacat
     1201 cagcggaatg atcgggctca ggaagttgac cgccgcggtc atgacgaact ggatgccgac
     1261 catcgcccac agcgtccgtt gccaatcggt gtcgccaggc cgcagcgcgg gataggatcc
     1321 aggcgggagg ttttccggcg cttccttcat cttcggtcat gctaaccgaa aggcagtgga
     1381 ttggaaggct gccaaggccg caatgggcca aggccgcaat gggtttgaca gtatccgagg
     1441 cgtctgggac attcccggcc tgaaggggag gaaaccaatg tcgtcgcgcc aggaccgcaa
     1501 gcggatcggc ctgctgctac cgtcgtccaa caccacccag gagcccgagt tcacccgcat
     1561 catgccctac ggcgtgacgt tgcacgtcgc ccgcctcccg ctccgcaccg tcgaacccgc
     1621 ctccaccgct cggattgtcg aggacatcga aaccgagagc caaaagcttg cagatgcgga
     1681 tgtcgatgcg atcgtgctgg cggcaacggc gccaagctcg cgcaacggca tcggatacga
     1741 ccaggaactg atcaagcgga tcgagactgc ctcgggcaag ccggccacaa ccgcctccac
     1801 cgcacttatc caggcgctga agacgttgga cgcaacccac ctggtggtcg gcgcaccctg
     1861 gagcgaggcg accaacgtca ccaccgcggc ttttgtccag gcgcacggct tcactgtgct
     1921 ggcgcatcag gcgatgggac atttaagcaa cctggagatc ggcgacctgg aggagcagac
     1981 cgcgtacgag atgggcatcg ccatcgaccg gcccgaggcc cgaaccgtga tgctggcttg
     2041 cggcaactgg ccgaccctgg ggatcgtgga ccgcctggaa tccgcgctcg gcaagccggt
     2101 gctgacgacc aaccaggtca gcctgtgggc ggtgcttcgg ctggttgggt atcacgcgcc
     2161 ggtcttcggt tggggattgc tgttgcggga gcacatgatc gagcctgcca gtgttgttct
     2221 agcgttatga aaatcgtcat cccggacgac taccaggata tcgtcgatcg actgtcctgc
     2281 ttctcgcttc tcgttggcca tgacgtcatc cgctatcgcg aacccgccgc cgatctggat
     2341 caactcgtcg aacgcctgca cccggccgag gtcgtcgtcg caatccgtga gcgggtgaat
     2401 ttctcccgcg cgttgctcca gcggttgcct aacctgaagc tgatcgcgtt ggtcggccga
     2461 gccgcgacga cgatcgacta tggtgcgtgc aaggattgcg gtgtcctggt gtccaccggc
     2521 gccagcaact ccccggtcgc gccggccgaa ctgaccgtcg cgctgatcgt cgcctcgcgc
     2581 cgcaatatcg cgttggaggc ggaacggatg cgccggggcg actggccatg cacgttatcg
     2641 catcgtctgg ccggcagcac gctgggcatc ttcggcctgg gttcgatcgg gtcgctcgtt
     2701 gcccgggccg gggcagggat ggggatgaat gtcctggctt ggggacagca ggcgtccgcc
     2761 gccaaggccc aggccgccgg ttatgatttc gccgccggca aagccgattt gttcgagcgt
     2821 tcggatgtcc tgtcgctaca cgtccgcctg cgtcccgata ccatcggtat cgtcggtccg
     2881 gaggatttgg cgcggatgaa acccaccgcg ctgctggtca actcctcccg agcggaactg
     2941 ataaagcgcg gtgcattgtt ggcggccttg gaggcggggc accccggtta tgccgcggtg
     3001 gacgtttatg aggaagaacc catcaccggc ggcaaccacc cattcctgtc gatgcccaac
     3061 gtcctgtgca cgccacacct gggatgggcc gagtgggaca atttcgagct ctatttccgg
     3121 gaatgtttcg aacagatcgt cgcctatttc gggggcgccg ccatgcgcct ggcgggcttg
     3181 ggctgacacc aggcgtggag cccctaaagc cgctccgcct ggcccctggt ccccgctggc
     3241 aatgtcctga gccgttccag taccgccgga acgccctcga tcacctcgaa caagccgcgg
     3301 caggcgggat cggcgaagcc ctgctcgatc gcgtggtcga tgaccgagac caaaggcgcg
     3361 gccgaaccgg cgacgttgca cagcatgatg ggtttgtcgt gcaggcgaag ctggcgccag
     3421 gtgatgattt cgaatgcttc gtcgaacgtg ccgaggccgc ctggcatgat caggaaggcg
     3481 tctgactcct catacaagcg gcgcttgcgg gtatgcatgc tgtcggtgat gaccatctct
     3541 gtaacgtatt cgtgggcgac ttcccactgg gtcaggaatt ccgggatcac gcccaggacc
     3601 tttccgccgg cttctagcac ggcatcggca acgatgccca tgatgccgat ccggccgcct
     3661 ccaaacacca aacggatatc tgcctgcgcc agaccaaggc ccagtgcccg tccggcggcg
     3721 gcataatcgg gtttgcggcc gacgcgggac ccgcaaaaaa cagcgagtga gtgtatcccc
     3781 gtcatgaata tccttctttc taacgtctta tagtctgttc gttggcaaaa aaatctgcta
     3841 ccgaatcatc gtccaacctg gatcattatg catgatgatc catcaaccaa atgggaaggc
     3901 tttaccggat gacaaaacga atagctattt atagtgccat tatgctggct gcggtgggta
     3961 tttcatcgat tggtgtcgct ttggctgatg gccttaatat cattcccatt cggcaagccg
     4021 gcatgtccct ggccggtggc gatttcgcct tcatccggtc gacggtagcg gccaaaggcg
     4081 atgtcaaggc gctcgaagct cccgccaagg ccattgcgaa atgggcggcc gtgatcccga
     4141 gtgtgttccc gaagggcacc gaaaccggca acgacaccaa agccctgccc gaagtatggg
     4201 ccgacaatgc cggattccag aagatcgctt tggcgttggg cgaagcctca accaagctgg
     4261 ccgccgccgc taaggccggc gatgctgatg aggttgccgc cgacgccaag ctggtgggcg
     4321 accagtgtgg cgcctgccac aagacctatc gcgcgaagta gcatctgatc cgctggatcg
     4381 ccggctacgg ttgtcaacga ccgcagccgg cgatctgccg gtttcgttgt cggtttttga
     4441 cgaaacatcg gtcttcgagg aaacgcggtg gtcctacgtg atccgcctcc aagccgaaga
     4501 caatctttcc ataccgtgtc ccgtcaacgg gccctttatc gtctcggcca gccccttgat
     4561 ggctgatgac aactgcctgt aacttgcgtg gcaacgctgt gacttgcgtg gccagcggaa
     4621 tgaacccacg ccgcatgatt gactccggtc tgcgaaaaag gcctactgct tatcaattaa
     4681 taattatctg ttgtgggttc gggaggaagc atgaggttgt ctatccggca tttttgccta
     4741 gcgttggtcg ctttgtcgat tgccataacg gctcgttcgg cctcggccga ggatacgatc
     4801 aaggtcggct atgtcgatcc gttctccggc ccattcgcct ccggtggcga cgaatttctc
     4861 aaagtattcg cttttatttt ggaaaaggcc aatgcgaagg gcggcgcgct tgggaaaaaa
     4921 ttcgagctgg tgccgttcga cgacaagctg cagccggcgg aagcgctgat cgccctgaaa
     4981 aacatcaccg atcagaacat cccgttcgtc atgcaatgca ctggatcgaa tgtcggcgcg
     5041 gctctggtcg atggcgtgtc gaagcacaat gcacgcaacc ccgacaaccg cgttctgtat
     5101 ttgaactgtg gcgcgctggc gaccgaactg accaacgaga agtgtgactt ctggcagttc
     5161 cgcttcgccg gaaacgtaga gatgcgcgcg gccgcccgga tcaaggcgct gcccaaggac
     5221 gtcaaaaagg tttacctgct caaccaggac tacctgttcg gccagtccgt gcaacatgat
     5281 acccgcaaat ggctggcgaa actgcgcccc gacatcgagg tggtgggcga cgaactgatg
     5341 ccgttcggca aggtgcagga tttctcgtcg tacgtcgcta agatcaaggc gtccggcgcg
     5401 cagtccgtca tcaccggcaa ctacaatcgc gacttgaacc tgttgatca
//
LOCUS       DASWQN010000722         3238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_334034, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000722 DASWQN010000000
VERSION     DASWQN010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334034"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1894)
                     /locus_tag="VGC82_12295"
     CDS             complement(242..1894)
                     /locus_tag="VGC82_12295"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0424092.1"
                     /translation="MAEITGSEIIARCLQKEGITDLFYIMGGPMLLAEATCIKEGIRM
                     IDVRHEQAAAFACQAYTRLKQAPSVCMAASGPGVTNLITGMANALIDCCPVVALGGSS
                     PLSQFGRQVFQEIDQVELMRGCCKYVDRLINLKRIPQQINFALQKAMSGKPGPVYLDC
                     PGDMLYQKIDENLVDWSFAGRPILDSRPMGDPRQVDALIGALAEAKNPVIVSGSGVIW
                     SRAWAEMQAFVEAAGIPFYTTPQGRGVVPDDHQYAYPSMRSGAFKDADLIIVLGTRMN
                     YIIGHASPPRFGPTAKIARIDIDPAEIAAAARYVDIPIVGDCKAVLGQLLQGIKGRIN
                     PDTYAGWRKQLSDGEAAKRSSAGANKLVEDGDIHPVRMLEAVRDFAKRDAILCVDGQE
                     TLNFGRLTMPTYAPGHRLNSGPFGTMGVGMPFGVGAKVACPDKQVIVVHGDGSMGLNA
                     MELDTAIRHKIPILVVISLNGGWTGDPKREKPGRDLGYTRYDKICEALGGYGEYITKV
                     EDIAPALERAQKKVDEGMVALVNVRTDYRARFAGVAFSDYST"
     gene            complement(1934..>3238)
                     /locus_tag="VGC82_12300"
     CDS             complement(1934..>3238)
                     /locus_tag="VGC82_12300"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0424093.1"
                     /translation="ESRGAYLTGFFVLALSECDKMGKSLMTTNGFGGDCLAGVRVLDM
                     TQFEAGPSCTETLAWLGAEVVKVENPKGGDPGRSLGGKDGMDDPYFLNFNANKKSISV
                     NLKDPHGLQLVKDLAAKADVLVENFAPGAIERLGLGAEVIRALNPSIIYCQVKGFGEG
                     SPYEKNLAFDMIAQACSGIMSTTGELDGRPLKPGPSLGDTGTGMLLSISILGALYRRK
                     ATGQGDHLQVAMQDAMLHYMRIAFSYQARTGKAAPRAADSSVSGGNPPMGTFPSKGGG
                     PNDYVYVYTSRANPEHWRRLLAVIGREDLIGDARYDSPSARSERAAEVNEMVAAWTRR
                     HTKHEAMEIIGAAGIPAGAVLDTQELTDDPTFEQRGIMQTIDHPVVGPFKMVAWPVRF
                     SGSPPPVKPAPLLGANNDDVLTNWLGLGADQVGGLRTDNIIG"
BASE COUNT          554 a         1031 c         1024 g          629 t
ORIGIN      
        1 cagccgagca gtaaagcact gtcccttggc gctgacagca gaagccgcag acgcgtccac
       61 aggattggta atgtaggcgc ggagcgtcag tagcattgtt aatgttcgcg cggatgcgtc
      121 aacagggttg ttaatgcgta cgtgtcatgg ccgggcttgg cccggccacc cacgacttca
      181 acgccgcccc aaatgcgaca gcggcagccg gaacgagctc cggccgccac gcacccggcc
      241 gctaagtcga gtaatccgag aacgccacgc cggcaaaccg cgcacggtaa tccgtgcgga
      301 catttaccaa tgccaccatt ccctcatcga ccttcttctg cgcgcgttcc agcgccgggg
      361 cgatatcctc cactttggtg atgtattcgc cgtagccgcc aagcgcctcg cagattttgt
      421 cgtaacgggt gtaaccaagg tcgcgacctg gcttttcccg cttcgggtcg ccggtccagc
      481 cgccgttcag gctgatcacc accaggatgg ggatcttgtg gcgaatggcc gtatccagtt
      541 ccatcgcgtt caatcccatc gagccgtcgc catgcaccac gatcacctgc ttgtccggac
      601 aggcgacttt cgcgccgacg ccgaacggca tgccgacacc catggtgccg aacggaccgg
      661 agttcagacg atggcccggc gcgtaggtcg gcatggtcag gcggccgaag ttcaaggttt
      721 cctgcccatc gacgcacagg atggcatccc gcttggcgaa gtcgcgaacc gcttccagca
      781 tgcggacggg atggatgtca ccgtcctcga ccagcttgtt ggccccggcg gaggaccgtt
      841 tcgcggcctc gccgtccgac aactgcttgc gccagccggc gtatgtgtcc gggttgatcc
      901 ggcccttgat gccttgcagc aactggccca gcacggcctt gcagtcgccg acgatgggga
      961 tatcgacata gcgtgcggcg gctgcgattt cggccggatc gatatcgatg cgggcgatct
     1021 tggcggtggg tccgaaccgc ggcgggctgg cgtggccgat gatgtagttc atgcgggtgc
     1081 cgaggacgat gatcaggtcg gcatccttga atgcgccgga gcgcatcgag ggataggcgt
     1141 attgatgatc gtccggcacc acgccgcggc cttgtggcgt ggtatagaac gggatgccgg
     1201 ccgcctcgac gaaggcctgc atttcggccc aggctcgcga ccagatcacg ccggagcctg
     1261 acacgatcac cggatttttc gcctccgcca gcgcgccgat cagcgcatcc acctggcgcg
     1321 gatcgcccat cgggcggctg tcgaggatgg gacggccggc gaacgaccag tccaccaggt
     1381 tttcatcgat cttctggtac agcatgtcgc cggggcaatc gaggtaaacc ggaccgggtt
     1441 tgccgctcat ggccttttgc agggcgaagt tgatctgttg cgggatgcgc ttgaggttga
     1501 tcagccgatc gacgtatttg cagcagccac gcatcaactc gacctgatcg atttcctgga
     1561 acacctggcg gccgaactga ctcagcgggc tggatccacc gagggcaacc accgggcagc
     1621 aatcgatcag cgcgttggcc atgccggtga tcaggttggt gacgccaggg ccggaggcgg
     1681 ccatgcagac cgacggcgcc tgcttcaggc gagtataggc ctggcacgcg aaggccgccg
     1741 cctgttcatg gcggacgtcg atcatgcgga tgccttcctt gatgcaggtg gcctccgcca
     1801 gcagcatcgg gccgcccatg atgtaaaaga gatcggtgat cccttccttc tgcaggcatc
     1861 gggcgatgat ttccgagccg gtgatttccg ccatattctt gttctcccta gattagccga
     1921 tgggtcgccg atattagccg atgatgttgt ccgtccgcag gccgccgacc tggtcggcgc
     1981 caagtccgag ccagttggtc agcacgtcgt cgttgttcgc ccccagtaat ggtgccggct
     2041 ttaccggcgg ggggctgccg gagaagcgga cgggccaggc aaccatcttg aacggtccca
     2101 ccaccgggtg atcgatggtc tgcatgatgc cgcgttgctc gaaggtggga tcgtcggtca
     2161 gttcttgcgt atcgagcacc gctcccgcag ggatgccggc ggcgccgatg atctccatcg
     2221 cttcgtgctt ggtgtgccgg cgggtccagg cggcgaccat ctcgttcact tccgcggcac
     2281 gttctgaccg ggcggagggg ctgtcatagc gcgcgtcgcc gatcaggtct tcccgcccaa
     2341 tcaccgccag caggcggcgc cagtgctcgg gattggcacg cgatgtgtac acgtagacat
     2401 aatcgttggg accgccgcct ttgcttggga aggtgcccat cggcgggttg ccgcctgata
     2461 cgctgctgtc ggcggcccgt ggggcggcct tgccggtgcg cgcctggtag gaaaacgcga
     2521 tgcgcatgta gtgcagcatc gcatcttgca tcgcgacctg cagatgatcg ccctgccctg
     2581 tcgccttgcg gcggtagaga gcgcccagga tcgatatcga cagcagcatc ccggtgccgg
     2641 tgtcgcccaa cgacgggccg ggcttcagcg gccggccgtc caattcgccc gtcgtgctca
     2701 tgatgccgct gcaggcctgc gcgatcatgt cgaacgcgag gttcttctca tacgggctgc
     2761 cctcgccaaa gcccttcacc tgacagtaga tgatcgacgg gttcagcgct cggatcacct
     2821 cggcgccgag gcccaggcgc tcgatcgcgc ccggcgcgaa gttctccacc agcacatcgg
     2881 ccttggcggc aaggtccttg accagttgca ggccgtgcgg gtccttcagg ttgacgctga
     2941 tggacttctt gttggcattg aagttcagga aatacggatc atccatccca tccttgccgc
     3001 ccagcgaccg gcccggatca ccacctttgg gattttccac cttcaccacc tcggcgccga
     3061 gccaggcgag ggtttcggtg caggacggtc cggcctcgaa ctgggtcatg tccagcacac
     3121 gcacgccggc aaggcagtcg ccaccaaacc cgttggttgt catgagcgat ttccccatct
     3181 tgtcgcattc gctcaacgct aggacgaaaa atccagtaag gtaagcccct cggctttc
//
LOCUS       DASWQN010000723         3255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_597704, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000723 DASWQN010000000
VERSION     DASWQN010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597704"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /locus_tag="VGC82_12305"
     CDS             complement(<1..1811)
                     /locus_tag="VGC82_12305"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY0424094.1"
                     /translation="MGDTKGRSYLPRTRKTRAMLAVLAMASPKPVLRVQLASLLWSRR
                     ENEQARASLRQSVHELQDTLGGTWSHVFVADRHHLSLRGTELDIDALMLAQPERISTG
                     LLDRYEDVLLEDLDGLDPAFDRWLDEERGRFVRIGRAIGESLLAQCDDPVATIEAAEH
                     LLGIDRRHESAWRAIIRANAEQGDFAAALGSYERCRSVLAESSAARPSPETEDLVGRI
                     RAEIVSDRLSGPVAPPTVAPVRVVRRDRTTLRLRVMPLRTIGEERDDGLGAGLAEEIS
                     AGLSRFRWISCLPTMVFPSGSIADRTSGGSLVEPGEGPWTGLEADLILDGTIQHGVGR
                     VRIIVRLMDMRAGGEIVWAGRFDREPTDTLALQDELGAAIVAQVDPELMQHEARRSSA
                     ARSHDLTAQDLLLQALPALYRLERDSFLNARRLLEMSLRSDSSSSVAHGWLAYWDLLY
                     IGQGWASNPAATTAEAARLAERAVMLDPGDARALTLAGHVRGFLGKRPEEANALHERA
                     IALNPNLALAWCFSGLAHSYLGRHEEALRRMNLALRLSPSDPHGFFYDTALIMPHLLR
                     GDYAGAIEAGRRAIEINPLFSSAYKGYLSALGWMDQKR"
     gene            2176..3228
                     /locus_tag="VGC82_12310"
     CDS             2176..3228
                     /locus_tag="VGC82_12310"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEY0424095.1"
                     /translation="MAVDGLTLPFQSRFATQAWLRRGLGLVLCIAIAALAEIGGSFAP
                     LIGAPLFAIGIGVLIANAVPAFVQQKSLRISDTSKLCLKTGIVLLGASLDLGDIVRTG
                     LGSLPLLAVTMTSGLICALWLGKSLGIDWRMRCLIGIGTTICGASAIAALAPVIRAKA
                     EEIAYSVTVVFFFNMVAVFTFPALGHLLGLSDNGFGIWAGTAVNDTSAVVAAGFAFSQ
                     AAGTTATIVKLTRTTLIIPLVIGFGLALPFLIKQQDAGSQSLAKRAYAAVPTFIILFV
                     LAAAANSAGLIGPHAMEIQMLGRVVMVVALAAVGLQGHWRAFVGAGSRPLVLGLATWF
                     VVAASSLAVQAWTQAL"
BASE COUNT          611 a         1031 c          988 g          625 t
ORIGIN      
        1 cgcttttgat ccatccagcc gagggcagag aggtaaccct tgtaggccga cgaaaacagc
       61 ggattgatct cgatcgcccg gcgcccggcc tcgatcgcac ctgcatagtc gccgcgcaac
      121 aggtgcggca tgatcagggc cgtgtcgtag aaaaacccgt gcgggtcgga cggcgacaag
      181 cgcagcgcca ggttcattcg gcgcaatgcc tcctcatgcc gacccagata agagtgcgcg
      241 agtccggaaa agcaccacgc aagggcaaga ttcggattaa gagcgatcgc ccgctcgtgc
      301 agcgcattgg cttcttccgg acgcttgccc agaaagcccc tgacatgccc cgccagcgtc
      361 aatgcccggg catcgcccgg atcgagcatg acggcacgct ccgccaacct ggccgcctcg
      421 gccgtggtgg cggcaggatt agatgcccat ccctgaccga tgtagagcag atcccaatag
      481 gccagccaac catgggcgac cgaactgctg gagtccgaac gcagcgacat ttcaagcaac
      541 cggcgggcat tcaggaaact atcccgctcg agccggtata atgccggcag agcctgcagc
      601 agcaaatcct gggcggtcag gtcgtgcgac cgggccgccg aactgcgtct tgcttcgtgc
      661 tgcatcagtt cgggatcgac ctgggcgacg atcgcagcgc ccagttcgtc ttgcagagcc
      721 agcgtgtcag tcggctcgcg gtcgaagcgg ccggcccaga cgatctcgcc gccggcccgc
      781 atatccatca gacgcacgat aatgcgaacc cgtccaaccc catgctggat cgtgccgtcc
      841 agtatcagat cggcttcaag gccagtccac ggcccctcac ccggctcgac cagcgaaccg
      901 cccgacgtac ggtcagcgat cgatccggac ggaaaaacca tggtcggcag acacgagatc
      961 catcgaaagc gggatagacc ggctgatatc tcctcggcca agccagcgcc aagtccgtcg
     1021 tcacgctctt ccccgatggt tcgcagcggc atgacgcgca gccgcaaggt cgtgcgatcg
     1081 cggcgaacaa cgcgcacggg agcgaccgtg ggcggcgcaa ccggaccgga caaacggtcg
     1141 gaaacgatct cggcgcgaat gcggccgacc agatcctcgg tttccggcga tgggcgcgcg
     1201 gccgacgact cagcgagaac gctccggcat cgctcatatg accctaatgc ggcggcgaaa
     1261 tccccctgtt cggcgttggc ccggatgatc gcccgccatg cgctctcgtg ccttcggtcg
     1321 atgccgagaa gatgctccgc ggcttcgatc gtggccaccg gatcgtcgca ctgcgcgaga
     1381 aggctttcgc cgatggcgcg tccgatccga acaaagcggc cgcgttcttc gtccagccag
     1441 cggtcgaacg ctggatccag accatcgaga tcctccagca atacatcctc ataccggtcc
     1501 agcaggccag tcgaaatccg ttcgggctgc gccagcatca atgcatcgat atcgagttcg
     1561 gtcccgcgca gcgacaaatg atgtctgtca gcgacaaaaa catggctcca ggtgccaccg
     1621 agcgtatcct gcagttcgtg cacactctgc cgcagagagg ctcgcgcttg ctcgttttcg
     1681 cgccggctcc atagcaacga cgccagttga acccgcagca ccggtttcgg gcttgccatc
     1741 gccaggaccg ccagcatggc ccgggtcttg cgcgtgcgcg gcaggtagga cctgcccttc
     1801 gtatcgccca cggtcatatg ccccaggaga cgcaagtgga ggcgggcacc gcagttcggg
     1861 gccatcgcgc ggtcgttggg aggtgcggtt tgccggcgaa tgacgagatc tcttatatga
     1921 tagcgaaagt catgacagat ctacaaccat aacgagagca acttactccc tagtatccga
     1981 tcacgggtta tagagcaaca atgctcgcgc tgaaacatca gcatcggccg cggcctttcg
     2041 gtgccattgg tgtcgttccg gtcagacttt ccgcgccgcg gaccggaaac gctaaattat
     2101 tttatgtttg gccagcttgg tgacacgcgc gcactcttcc cgcagtatcc ctgcaagatg
     2161 gacaaggagg aaaatgtggc ggtcgacggc ctcacgttgc catttcaatc ccggttcgcg
     2221 actcaggcat ggcttcgccg tggcctgggc ttggtgctgt gcattgctat cgccgcactg
     2281 gcagagatcg gcgggtcgtt cgcacccttg atcggtgctc ctttgttcgc catcggtatc
     2341 ggcgtgctga tcgcgaacgc cgttccggcg tttgtgcagc aaaaatcgct gcggatctcc
     2401 gacaccagca agttgtgcct gaagactggc atcgtcctgt tgggtgccag ccttgatctt
     2461 ggcgacatcg tccgcactgg acttggctcg ctgcccttgc tggcggttac aatgacatcc
     2521 gggctgatct gcgctttatg gctcggcaaa tcgctcggta tcgactggcg gatgcgctgt
     2581 ctgatcggaa tcggcacgac gatatgcggg gcctcggcga ttgccgcctt ggcgccggtg
     2641 atccgcgcca aggccgagga gatcgcctac tccgtgaccg tcgtattctt cttcaacatg
     2701 gtcgccgtct tcaccttccc ggcgcttgga catctcttag gtctgagcga caatggtttc
     2761 ggcatctggg cgggaacagc ggtgaacgac acctccgccg tggtcgccgc ggggttcgcg
     2821 ttcagccagg ctgccggcac gacggccacg atcgtcaaat tgacccgaac aacgctcatc
     2881 attccgttgg taatcgggtt cggactagcg ttgccattcc tgatcaagca gcaggacgcc
     2941 gggtcccaat cattggccaa gcgcgcttat gccgctgttc cgacctttat catccttttc
     3001 gtgctggcag ccgccgcgaa cagcgccgga ttgatcggtc ctcatgccat ggaaatccag
     3061 atgctgggcc gtgtggtgat ggtcgtggca ctggcggccg ttggactgca aggtcattgg
     3121 cgggcctttg tgggtgccgg cagccgaccg ctggttttgg gcttggcaac atggttcgtc
     3181 gtggcggcat ccagcctggc ggtgcaagcc tggacgcaag cgctttgagt tcacgaaatc
     3241 gttttcgcgg ctgta
//
LOCUS       DASWQN010000724         3667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_465493, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000724 DASWQN010000000
VERSION     DASWQN010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465493"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..1423
                     /locus_tag="VGC82_12315"
     CDS             299..1423
                     /locus_tag="VGC82_12315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0424096.1"
                     /translation="MEDALEDFPASDKLIGAIDCDIHPAVPSTRVLLPYLDDYWREHV
                     LRRGLERENLEPSSYPVNAEINGRPDWLLPSGPPGSDLVAMQSHLLDRLRPSFAICNV
                     IHGAQVMLSEDLSAAFCKAINNWIRAEWLDRDPRLRASIVVPPHSAELAVQEIERLAP
                     DHRFVQVLLLAMTELPLGRRQNWPIYKAAEAHGLPIGIHAGSSFRHPPSALGWPSYYL
                     EDYVSQAQGFQAALNSLVSEGVFVKFPGLKVVLIESGVTWLPASLWRLDKTWRGVRAE
                     VPWLEQLPTATVREHVRLTLQPFDAPPNEQQLATLLEQLGSDRMLLFSSDYPHWHFDG
                     DDALPAGLSAELVRKICVDNPLETYPRLKHDRAPRTVDGA"
     gene            1545..2657
                     /locus_tag="VGC82_12320"
     CDS             1545..2657
                     /locus_tag="VGC82_12320"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0424097.1"
                     /translation="MNMTVLDRKPDLGAASHLGFIDCDVHPYVKGPNDFDPFLSKRWL
                     EHKRTIGGRTRQGLAKTAMYPRMSPGVGMRMDSWPKTGGHPGSDLPMMQEQLLDLFDV
                     AYGLLAPLVGGAGGERNVEFGAAMATAVNEWQIAHWCDPDPRLLGAVQVNVEHTEAAI
                     AEIEKRAGDRRFAQVNIPPRGLEPLGRRRYWPILEACAANGFPVSLHLGGTSGHASTG
                     GGWPSFYHEEHPSYVQTMQTLVTSLVCEGVFEQIPNLKIVLVEGGFAWLPALCWRLDK
                     HWKRLKAEVPHLTRSPSEYVHEHFWVTTQPIEEPERPQDMLSILEWIGPERVMFSTDY
                     PHWDQDDPRYAFKIALPDDWKRKIYRDNARALYNLD"
     gene            2691..3104
                     /locus_tag="VGC82_12325"
     CDS             2691..3104
                     /locus_tag="VGC82_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY0424098.1"
                     /translation="MSRHVVARVEDIPLGCRKIVTVRGREIGIFNVGDSFYGLINRCP
                     HQGAPLCTGEIVSRLVAPTPGDYRLTRPGEMIRCPWHCWEFDIRTGQSLCDPDSVQAR
                     ALDIAVEPGATLVEGPFVAESVEVSVEASYVVVTM"
     gene            3192..3335
                     /locus_tag="VGC82_12330"
     CDS             3192..3335
                     /locus_tag="VGC82_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424099.1"
                     /translation="MLQYLIANLAGDLCFDPITAEAVALAVSGLQQPAAAGLEAAIVR
                     SLS"
     gene            complement(3447..>3667)
                     /locus_tag="VGC82_12335"
     CDS             complement(3447..>3667)
                     /locus_tag="VGC82_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424100.1"
                     /translation="VASRKVRRVLGPLRLVLIALAAGVVVLSQPAAPVQAQLMDQLKG
                     AVGSGQGETNNSGGILGGLGGGGMPSVG"
BASE COUNT          669 a         1127 c         1172 g          699 t
ORIGIN      
        1 tggcttccgg tcttgcgaac gctccgggtg gatctgtgga acgggggcgg cttttggttt
       61 ctcgctcgca gggcgtattc gatgcgtcgg gaacgatgat cgacgcgggc gcgaaagagc
      121 aactccgcgg cttcatgggt gggttcgccg gcttcgtcgc gctgcacaaa acatagcgca
      181 agcctacacg cgacgtgtgt tgatccagga gggccgcgtc actgccgccg tgcgatcgtt
      241 tacacgcaac ccggcatcgc ttaccctccg gtggcataac tgaaggcgaa gaccgaagat
      301 ggaggacgcg ctggaggatt ttcctgcctc cgacaagttg atcggcgcca tcgactgcga
      361 cattcatcca gccgtcccca gcacccgcgt gttgctgccc tacctggatg actattggcg
      421 tgagcatgtc ctgcgccgcg gtctggaacg agaaaacctc gaaccgagca gctatcccgt
      481 caatgccgag atcaacggcc gccccgactg gctgctgccg tcgggtccac cgggcagcga
      541 cctggtggcc atgcaatccc atctgctcga tcgcctgcgc ccaagctttg cgatctgcaa
      601 tgtgatccat ggcgcccagg tgatgctgag cgaggatctg tccgcggcgt tctgcaaagc
      661 gatcaacaac tggatcaggg cggaatggct ggaccgtgat cccaggctgc gtgcgtccat
      721 cgtggtgccg ccgcacagcg cggaactcgc ggtccaggag atcgaacgcc tggcgccgga
      781 ccatcgcttc gttcaggttc tcctcctggc gatgacggag ttgccgctcg ggcggcggca
      841 gaactggccg atctacaagg ccgcggaagc gcatggattg ccgatcggca tccacgccgg
      901 cagcagtttc cgccacccgc cgagcgcgct gggctggccg tcctattatc tggaggatta
      961 tgtttcccag gcccaaggct ttcaggctgc gttgaacagt ctggtcagcg agggcgtctt
     1021 cgtgaaattt cccggcctga aagtggtgtt gatcgagtcc ggcgtcacct ggctgccggc
     1081 gtcgctatgg cggctggaca agacatggcg cggcgtgcgg gctgaagttc cttggctgga
     1141 gcagttgccg acggcaacgg tccgggaaca tgtgcggttg acgctgcagc cgttcgacgc
     1201 cccgcccaac gaacaacagc ttgctacgct gctggagcag cttgggtccg accggatgct
     1261 gctgttctcc tccgattacc cacactggca tttcgacggt gacgacgcgc tgccggccgg
     1321 gctgtccgcg gaactggtga gaaagatctg cgtcgataac ccgctggaaa cctatccccg
     1381 gctaaagcat gatcgcgctc cgcggaccgt ggacggcgcg tgaatcatgc gatcgaacaa
     1441 caagcatgat cgcgctctgc ggaccgcgga aggcgcgtga atcatgcgat caaacaactc
     1501 aggctgctcg gtcagcctga aaatcgcctg aaggagacga cgcgatgaat atgactgtcc
     1561 tggaccgcaa gcccgatctt ggcgccgcga gccacctggg attcatcgac tgcgacgtgc
     1621 atccctatgt caaaggtccc aatgatttcg atccgttcct gtcgaagcgc tggctggaac
     1681 acaagcggac gatcggcggc cgcactcgcc agggcctggc gaaaactgcg atgtacccgc
     1741 gcatgtcgcc cggggtgggg atgcggatgg atagctggcc gaagaccggc ggtcatcccg
     1801 gatccgacct gccgatgatg caggaacaac tcctggacct gttcgatgtc gcctacggat
     1861 tgcttgcgcc actggtcggt ggagcgggcg gtgagcgcaa tgtcgagttt ggcgctgcga
     1921 tggcgacggc ggtcaatgaa tggcagatcg cgcattggtg cgatcccgat cctcggttgc
     1981 tgggcgcggt tcaggtcaat gtcgagcata ccgaagcggc aatcgcggaa atcgagaaac
     2041 gtgccggcga ccgccgcttt gcccaggtga acattccgcc tcgcgggctg gagccgctgg
     2101 ggcgtcgccg ctactggcca attctggagg cttgcgcggc gaacggattt cccgtgtcgc
     2161 tgcacctcgg cggcaccagc ggccacgcat ccaccggcgg cggctggccg tcgttctacc
     2221 atgaagagca tccgtcctac gtgcagacga tgcagaccct ggtgaccagc ctggtttgcg
     2281 agggcgtgtt cgagcagatc ccaaacctga agatcgtgtt ggtggaaggc ggcttcgcct
     2341 ggctgccggc gttgtgctgg cggttggaca agcattggaa gcgcctgaag gcggaggtgc
     2401 cgcacctgac gcgctccccg tcggaatatg tgcatgagca tttctgggtg acgactcagc
     2461 cgatcgagga gcccgagcgg ccacaggata tgctgtccat cctggaatgg atcggaccgg
     2521 agcgggttat gttctcgacc gactatccgc actgggatca ggatgacccc aggtacgcat
     2581 tcaagatcgc gctgccagac gactggaagc gcaagatcta ccgggacaac gcccgggcct
     2641 tgtacaatct tgactagggc aggggaggcc tggactaggg caggggaagc atgagccgcc
     2701 acgtcgttgc cagggtggag gacatcccgt tggggtgtcg caagatcgtc accgtgcgag
     2761 gccgcgagat cggcatcttc aacgtcggcg actccttcta tggcctgatc aaccgctgtc
     2821 cgcatcaggg cgcgccactc tgcaccggtg agatcgtcag ccggctggtc gcgcccaccc
     2881 cgggcgacta ccgtctgacg cgcccgggcg agatgatccg ctgtccctgg cactgctggg
     2941 aattcgacat ccgcacgggg cagtcgttat gcgatccgga tagcgtgcaa gcgcgagcac
     3001 tcgacatcgc agtcgaaccc ggcgccacac tggtcgaagg acctttcgtt gccgaatcgg
     3061 tcgaggtgtc ggttgaggcg agctacgtcg tcgtaacgat gtgacgtgtt gaatggctgg
     3121 agcgggttcg aggttcgatc ctgctaccgg gtcgaagcat ccagtcattc acgtcaaagc
     3181 agcgacttcg catgctgcag tatctgatcg caaaccttgc gggtgacctg tgctttgatc
     3241 ccatcaccgc cgaggctgta gccctggccg tttccggtct gcagcaaccc gctgctgccg
     3301 gccttgaagc cgctatcgtt cgctccctgt cctgatccgg tcattttact cataagcgag
     3361 ttcttgaccg acgatgcgga gctgtcgccg acgtagttgt tctgaataca atactgcagg
     3421 acaccggccg tattgctggg gccggcctaa ccgaccgagg gcattcctcc gccgcccaac
     3481 ccaccgagaa tgccgccgct gttgttagtc tcgccttgcc ccgatccaac cgcacccttc
     3541 aactgatcca tcagttgtgc ctgcaccggg gcggccggtt gcgatagcac gacgactccc
     3601 gctgcgagcg cgatcaggac gagccgcagc ggacccagca cgcgcctgac tttacgagat
     3661 gccacca
//
LOCUS       DASWQN010000725         3783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2577513, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000725 DASWQN010000000
VERSION     DASWQN010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2577513"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..633
                     /locus_tag="VGC82_12340"
     CDS             253..633
                     /locus_tag="VGC82_12340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0424101.1"
                     /translation="MSANTILVAEDEPSTLETLGRALLDAGFAVLQADKASSAVALFE
                     SNDVDLLLTDIALPGPLDGVALAEWMRQRKPHLPVVFLSGHFNRLATADGLPPPSAFL
                     VKPVSLEEVVSVVARLLDEAATGR"
     gene            864..2591
                     /gene="poxB"
                     /locus_tag="VGC82_12345"
     CDS             864..2591
                     /gene="poxB"
                     /locus_tag="VGC82_12345"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HEY0424102.1"
                     /translation="MPRQSVADIIADTLHNVGIRRIYGIVGDSLNGLTDSLRRKRNID
                     WIHTRHEESAAFATGAEAHLTGQLAVCAGSCGPGNLHLINGLYDCHRSRVPVLAIAAH
                     IPTDEIGSGYFQETDPRHVFAECSHYVGPVATVSQIGRTLDIAIREAVAKRGVSVVVI
                     PGDVALQQAEQPAPARWIAPAAPLVHPNVEDLQALAQMLDGAQRVTMLCGAGCAGAHG
                     EILALAEMLKAPVVHALRGKEWVEYDNPYDVGMTGLIGFSSGYHAMMNCDALLMLGTD
                     FPYRQFYPAQAKVAQVDLRGENIGRRCHVDVGVVGDVKLTAMALRGLLTAKTDRSYLD
                     RAVAHYRDARKSLDELAVGHPGRKPIHPQYLARVLDEVASDDAIFTCDVGTPTVWAAR
                     YFTMNGRRRLIGSFWHGSMANALPQAIGAQAAFPNRQVVSFSGDGGFAMLMGDLITLK
                     QAELPAKVVIVNNGTLGFVELEMKASGFLDTGTELQNPNFASLAESCGILGIRVEDPA
                     DLRDALVRALRHPGPAVVDVVTNRMELSMPPKATMEQAKGFGIYLAKAIISGRGDEIV
                     ELARTNLWR"
     gene            2603..3346
                     /locus_tag="VGC82_12350"
     CDS             2603..3346
                     /locus_tag="VGC82_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY0424103.1"
                     /translation="MRVALFVPCYVDAFEPQVGVATLELLERFGLDVAYPFDQTCCGQ
                     PMTNTGCHQEAAATEALFVRNFTGYDYIVAPSGSCVHQVRAHMTAIEQTQEVIEVRNR
                     TFELVEFLHDVLKVEAFPWAAFPHKVSYHGNCNALRGIGHASPSELHEPFFSKPLDLL
                     AKVKGVELVQIVRPDECCGFGGTFSVFEPAVSAKMGHDKVADHVRAGAEYVVSADSSC
                     MMHQRGSADRLGLKLKFIHIAQVLNGAAA"
     gene            3343..>3783
                     /locus_tag="VGC82_12355"
     CDS             3343..>3783
                     /locus_tag="VGC82_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424104.1"
                     /translation="MSKLLHPDLHWGKNEAPVNQDGERLQHAEGTSQPLRPNRNEPQP
                     RGAKVHGRRPIDQNEAAARFIAAPEHAKMHDERLWDLRKKRDAQMHGIPEWEDLRELA
                     SQIKQHTLGHLDRYLEQFEENAKANGVHVHWAVDGAAHNQIVHGI"
BASE COUNT          732 a         1178 c         1192 g          681 t
ORIGIN      
        1 gatgatctgc gcaaaaccgc cgcccatggg ctcggctcgg cggtcggaac ctgcgtgaga
       61 catcaaccgg taacgccacc tttccgatgt cgccaacgtt cgccgcgggc cgccacttac
      121 aacattgggt gataccgata agccccatgg acacggcggc gttcagtacc cgcccgaagg
      181 caaccgattt tcacgttgag gaaacctgtt tggcctcgtg ctgttgctga cagacgtcgg
      241 tcggagatat tgatgtccgc gaacacgata ctggttgcgg aggacgagcc atcaaccctt
      301 gaaaccctgg gacgtgcgct tctggatgct ggcttcgcag tactccaggc ggacaaggca
      361 agttccgcgg tggcgttgtt tgagagcaac gacgtggacc tgttgctaac cgatatagcc
      421 cttccggggc ccctcgacgg cgttgcgctg gctgaatgga tgcggcagcg gaagccccat
      481 ttgcccgtgg tcttcctcag cggtcatttc aaccggctgg cgacggcgga tggactaccg
      541 ccgcccagcg ctttcctggt taagcccgta agccttgagg aggtcgtgag cgtcgtcgct
      601 cgcctgctcg atgaggcggc aacgggcagg tgactcgcga catcaacgac gacagcgcgc
      661 gtgcatatga cgccactgtt gatcctccga tgggtacggt gcggtagcgg tcctaagtca
      721 cactacgatt tgaaggcttg aggctgtcac gctgctggtg ccgcgcgcgt tgcgcaaaca
      781 gcagcgtagc ggttaacgtt tgtcgccggt gcgctagcgg acgaaacgcc tgtcacgggc
      841 cattcacttc acggacgatc cccatgccca ggcagtcagt cgccgacatc atagcggaca
      901 ccctgcacaa cgtcggcatc cgacgaatct acggcatcgt cggcgactcg cttaacggac
      961 taaccgactc gctgcggcgc aagcgcaaca tcgactggat ccataccagg cacgaagagt
     1021 cggcggcttt tgccacgggc gccgaagcgc acctgaccgg acaactcgct gtctgcgccg
     1081 gaagctgcgg cccgggcaac ctgcacctga tcaacgggct ctacgattgc caccgcagcc
     1141 gtgtcccggt gctggcgatc gccgcgcaca tcccaacgga cgagatcggc agcggctatt
     1201 tccaggaaac cgatccgcgt catgtgtttg ccgagtgcag ccactacgtc ggcccggtcg
     1261 ccacggtctc gcaaatcgga cgcaccctgg acatcgcgat ccgcgaggcg gtcgccaagc
     1321 gcggcgtctc ggtcgtcgtg atcccaggcg acgtggcttt gcagcaagcg gagcagccgg
     1381 cgcccgcccg ctggatcgcc cccgccgcac cactggtgca cccgaacgtg gaggacctgc
     1441 aggcgcttgc ccagatgctc gacggcgcgc agcgggtgac catgttgtgc ggggcggggt
     1501 gcgccggcgc ccatggggag atcctggccc tggcggaaat gctcaaggcg ccggtggtgc
     1561 acgcgttgcg cggcaaggag tgggtggaat acgacaatcc gtatgacgtc ggcatgaccg
     1621 gcctgatcgg attctcgtcg ggctaccacg cgatgatgaa ttgcgacgcg ctgctgatgc
     1681 tggggaccga cttcccgtac cgccagttct atccagccca ggcgaaggtc gcgcaagtcg
     1741 atctgcgcgg ggaaaatatc ggccgccgct gccatgtgga tgtgggcgtc gtcggcgatg
     1801 tcaagctgac cgcgatggcg cttcgggggc tgctgacggc gaagacggac cgctcgtatc
     1861 tggaccgagc cgtcgcgcac tatcgggacg ccaggaagtc gctcgacgaa cttgcggttg
     1921 gccaccctgg ccgcaagccg atccatcccc agtacctggc gcgcgtgctg gatgaggtcg
     1981 cttcggacga tgcgatcttc acctgcgacg tcggcacgcc gacggtttgg gcagcccgtt
     2041 acttcacgat gaacggccgc cgccgcctga tcggctcgtt ttggcatggc tccatggcca
     2101 atgcgctgcc acaagcgatc ggcgcgcagg cggcattccc gaaccgtcag gtcgtctcat
     2161 tctccggcga tggcggcttc gccatgctga tgggcgacct tattacgctg aagcaagccg
     2221 aactgccggc gaaggtcgtc atcgtcaaca acggcacgct aggctttgta gaactggaga
     2281 tgaaggccag cggcttcctc gataccggga ccgaactgca gaacccgaac ttcgccagcc
     2341 tcgcggaatc ctgcggcatc ctgggcatcc gcgtcgaaga ccccgccgac ctgcgcgacg
     2401 cgttggtccg tgcgctgcgc catccaggac cggcagtggt cgatgtggtc accaatcgga
     2461 tggaactgtc catgccgccg aaggcaacga tggagcaggc caagggcttc ggcatctacc
     2521 tggccaaagc aatcatcagc gggcgcggcg acgaaatcgt tgaactggca cgcaccaatc
     2581 tgtggcggta acggagatcc gcatgcgcgt tgccttgttc gtcccctgct acgtggatgc
     2641 attcgagcca caggtcgggg ttgccacgct ggagttgctg gagcggttcg ggttggatgt
     2701 ggcatacccg tttgaccaga cctgctgtgg ccaacccatg acgaacaccg gttgccacca
     2761 ggaagccgca gccaccgaag ccttgttcgt gcgaaatttc accgggtacg actacatcgt
     2821 cgccccatcg ggctcctgcg tgcatcaggt gcgcgcgcac atgaccgcta ttgagcagac
     2881 acaagaagtc atcgaagtac ggaaccggac attcgaactc gtggagttcc tgcacgacgt
     2941 gctgaaagtc gaagcctttc cttgggcagc attcccgcac aaagtcagtt accacggcaa
     3001 ctgcaacgcg ctgcgcggca tcgggcatgc ctcaccttcc gaattgcacg aaccattttt
     3061 ttccaagccg ctggacctgc tggcgaaggt caaaggggtg gaactggttc aaatcgtccg
     3121 ccccgacgaa tgctgcggtt tcggtggaac attctctgtg tttgagcccg cggtgtcggc
     3181 gaagatgggt cacgacaagg tggccgacca cgtccgagcc ggggcggaat acgtggtttc
     3241 agctgattcg tcgtgcatga tgcaccagcg cggcagcgcc gacaggctcg gactgaagct
     3301 gaagttcatc cacattgccc aggttcttaa tggagcggcg gcatgagcaa gctgctccat
     3361 cctgatctgc actggggtaa gaacgaagcg cctgtcaacc aggatggcga gcggctgcaa
     3421 catgccgaag ggacgtctca gcccctccgc cccaaccgca atgagccaca gccgcgcggt
     3481 gcgaaagtgc atggacggcg gccgatcgac cagaacgagg cggctgctcg tttcatcgcc
     3541 gcccccgaac acgccaagat gcacgacgag cggctgtggg atctgcgcaa gaagcgcgat
     3601 gcgcagatgc acggtattcc ggaatgggaa gaccttcgcg aacttgcctc gcagatcaag
     3661 caacatactc tcgggcatct tgatcgctac ctggagcagt tcgaggaaaa tgccaaagcc
     3721 aacggagtcc atgtgcactg ggcagtggat ggcgcggcgc acaaccagat cgtgcatggc
     3781 att
//
LOCUS       DASWQN010000726         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1257811, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000726 DASWQN010000000
VERSION     DASWQN010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257811"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1622)
                     /locus_tag="VGC82_12360"
     CDS             complement(<1..>1622)
                     /locus_tag="VGC82_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0424105.1"
                     /translation="TTAVIGNLSDDAAQVTWPATIRTLLRVVRPYRTQLAVTVLTGIG
                     RVIAFIGVSVLGALVLAAVRNGTPTGMLIAALLITAPLAGILHWLESWIAHAMAYALL
                     ADMRVDLFRKLDALAPAYLLRRRSGDLVALATQDVETVEYFFAHTIAPAIVAVLVPVS
                     VIVAMAWIAWPLALALAPFLLYAAASPVRGRQRIDRLGSAARGALGIMGAFTTETILG
                     LAVLVMFSATERWREGFMTAVRTYQGLRLSLLADLSFQAASLDALTGLGGLSVAALGA
                     VLVTHGMIAPTALPLLVLISVAAFLPVSEIAQVGRQLADTIASTRRLAVVHAEPVPIT
                     DGARDPADGSDVRFEHVGFVYPGRSMPALRDMSFTVPAGATVALVGPSGAGKTTVANL
                     LLRFWDPASGVIRLGGIDLRDLKLDGLRGRIALVAQDTYLFNDTLGANVRLARPEAPD
                     EQVALALSRAALAEFVAGLPEGLATRVGERGVQLSGGQRQRIAIARAFLKDAPLLVLD
                     EATSHLDTISEQAVRGALDTLMADRTTIVIAH"
BASE COUNT          303 a          564 c          536 g          219 t
ORIGIN      
        1 gatgcgcgat cacgatcgtc gtgcggtccg ccatcagcgt gtccaaggca ccccgcacgg
       61 cctgttcgct gatggtatcg aggtgcgacg tcgcctcgtc caggaccagt agcggcgcgt
      121 ccttcaggaa cgctcgggcg atcgcgatgc gctgacgctg gccacccgat agctgcacgc
      181 cgcgctcccc cacccgcgtt gccaatcctt ccggcaagcc cgccacgaac tccgccagtg
      241 ctgctcggga cagcgcgagt gcgacctgct cgtccggcgc ttcgggtctg gccagccgga
      301 cgttggcccc cagcgtgtcg ttgaacagat aggtgtcctg cgccaccagg gcgatgcggc
      361 cgcgtagccc atccagttta agatcccgca aatcgatgcc gcccaaccgg atcacgccgc
      421 tcgccggatc ccagaagcgc agcagcaggt tcgcgaccgt cgtcttgccg gcaccggatg
      481 ggccgaccaa agccacggtg gccccggccg gcacggtgaa cgacatgtcc cgcagcgcgg
      541 gcatcgaccg gccggggtaa acgaagccaa cgtgctcgaa gcgcacgtct gaaccatctg
      601 ccgggtcacg ggctccgtcg gtgatcggca ccggttcggc gtgcacgaca gccaggcgcc
      661 gggtggaggc gatcgtgtcc gccagttgcc ggccgacctg cgcgatttcc gagaccggca
      721 ggaacgccgc gaccgagatc agcaccagca gcggcaaagc cgtgggcgcg atcatgccgt
      781 gcgtcaccag cacggcaccc agagccgcga ccgacagacc gccaaggccg gtgagggcat
      841 ccagcgaggc cgcctggaat gacaggtcgg ccaggagcga cagcctcagg ccctggtagg
      901 tccgcaccgc cgtcatgaac ccttcgcgcc agcgttcggt ggcgctgaac atcaccagta
      961 ccgccaggcc taggatggtt tcggtggtga acgcccccat gatgccaagc gcgccgcgtg
     1021 ccgccgatcc cagccggtcg atgcgttggc gtcctcggac cggcgaggct gccgcgtaca
     1081 gaagaaacgg tgcgagggcc aaggccagcg gccacgcgat ccaggccatc gccacgatca
     1141 cgctgaccgg caccagcacg gcgacgatcg ccggcgcaat ggtgtgggcg aagaaatact
     1201 cgacggtttc cacatcctgc gtcgccagcg ccaccagatc gccggaacgc cgccgcaaca
     1261 ggtaggcggg cgccagcgcg tcgagtttgc ggaacagatc gacccgcatg tcggccagca
     1321 aagcataggc catggcatgc gcgatccagg attccagcca gtgcagtatc ccggcgagcg
     1381 gtgcagtgat cagcaaggcc gcgatcagca tgccggtcgg ggtgccgttg cgcaccgcgg
     1441 ccaacaccaa tgcgccgagg acgctgacgc cgatgaaggc gatcacgcgg ccaatcccgg
     1501 tcagcacggt aacagccaac tgggtccggt atggccggac gacccgcagc aaagtacgga
     1561 tggtcgccgg ccacgtaacc tgggcggcat catccgacag attgccaatg acagcggtgg
     1621 tg
//
LOCUS       DASWQN010000727         5344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_598249, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000727 DASWQN010000000
VERSION     DASWQN010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598249"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VGC82_12365"
     CDS             <1..418
                     /locus_tag="VGC82_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0424106.1"
                     /translation="RLLYTDTAMYVGVVYTYLPFMVLPLYARLSRLDATLMAAAADLG
                     APPSRVFLRVTLPLSLPGVAAGAALVFVPAIGEYVIPALLGGPRAQLIGKVLWEEFFA
                     NRDWPTASAVAVWLLLLLVAVPGAGIRMAGLFRRRI"
     gene            546..1022
                     /locus_tag="VGC82_12370"
     CDS             546..1022
                     /locus_tag="VGC82_12370"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY0424107.1"
                     /translation="MTSSMTGVLEAATRNANSSNDLALAAAQVIARRAALGLAAIFDP
                     MGADHVEFGRMVPEKVEAFSAAGMIMMRHATQAGEQIAQFASDAVMAAVRATVAIVGS
                     ASPAAMAETQGQFAKSWFEQAASNFMAIGILALGAQEAALVPIQETLAANSARLGG"
     gene            1277..2608
                     /locus_tag="VGC82_12375"
     CDS             1277..2608
                     /locus_tag="VGC82_12375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424108.1"
                     /translation="MASNVAATVEPIEKETMRRVARRLIPLLMAAYFAAYLDRVNVGF
                     AALTMNKSLGLSAEIFGIGSGIFFLGYFLFEVPSNLILSKVGARRWIGRILLTWGVIS
                     AATAFITGTWSFLGIRFLLGLAEAGFYPGIILYLTWWFPSNYRSRIIGIFMTAIPISI
                     VSGSLISSQILRFGAWGGLDGWQWLFILEALPAIVLGVVVIVYLTDTPEQAQWLEPAQ
                     RDWLVRRLGDERSQKEAVHNYSFAETVRNSRVWLLTLVYFGQNVTGYGLVIFLPQIVS
                     RFGVGVGMNGLISALPYLAAAMAMVWWGLHADRSAEHRLHAAAACFLNFAGLAACVFL
                     ENPLFMMIAIVLGQVGQSAIAPTFWALPTAMLSGTAAAGGIALINSVGNLGGFLGPYM
                     MGSIRDATGSFTIGLLSIATGALVAAIVLLLLPREQHLRQDSESNVRAAAD"
     gene            2687..3871
                     /locus_tag="VGC82_12380"
     CDS             2687..3871
                     /locus_tag="VGC82_12380"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0424109.1"
                     /translation="MTQPTGPLRGIRVIDITTVVLGPYCSQTLGDMGADVIKVETIEG
                     DSTRLIGPSRTPGMGSYFANLNRNKRSLAIDLKKPEAQEAILRLVETADVFVHNMRIG
                     AAQRLGLDYERLSARNPKLIYACASGFRKGSSMQEFPAYDDLIQGVSGIASLNAGPDG
                     APRYFPTVVVDKLTGAMLASMIGMALFHRERTGQGQEIHLPMMETILSFSLVEHLWHG
                     TLGEPEKGLGYPRMLTPHRRPYATKDGFISVIAHSDRQWAKLFQAMGVPELIEDVRFA
                     SVTARSANIDAVYATLTNGMRQRTTEEWLAELRPADIPCGKANSLNDLFTDPYLVETG
                     YFEPHVHPSEGSVVIPAIPAHFSGSPANVHRPWPKLGEHSREILAEAGFSDAEIESVL
                     SR"
     gene            4495..>5344
                     /locus_tag="VGC82_12385"
     CDS             4495..>5344
                     /locus_tag="VGC82_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424110.1"
                     /translation="MATFSWNTGTNGLWKIATNWTPGTVPNDIPPTATVDVTIDAKAT
                     TAAYLVTIAATDTITINSLTMNAVNNAQTLNQTPFLGAILEVDGTLKFAAGSAGLIDG
                     PLQNDLVVSNGTIVNAGTINAFVQGTGVSTFSGNNAIYFTNWLQSLGTVIVDTASIAE
                     FNAITNTLTDGIFEAKGSTNAVDLGGSLGHLIVNIGTIQGPGILLPGTNIEGWTQLIF
                     DGPDSTINEWNGTTYVPVESTIRLIQNNATISVNSDPISNLGRDYTTANALTIGAQAL
                     FFEQAGT"
BASE COUNT         1023 a         1614 c         1626 g         1081 t
ORIGIN      
        1 gcggctgcta tacaccgaca ccgcgatgta cgttggcgtg gtgtacacct acctgccctt
       61 catggtgttg ccgctttacg cccgcctgtc gcgactggac gcgacgctga tggcggcggc
      121 ggcagacctg ggcgcacccc cctcgcgcgt attcttgcgg gtgaccttgc ctctgtcgtt
      181 gccgggagtc gcggcgggcg cggcgttggt gttcgtccca gcgatcggcg aatacgtgat
      241 cccggcgctg ctcggcgggc cgcgggcgca gttgatcggc aaggtcctgt gggaggagtt
      301 tttcgccaac cgcgactggc cgacggcatc cgctgtcgcg gtttggttgc tgctgctgct
      361 ggttgccgtc cctggcgcgg gcatcaggat ggccggtttg tttcggcgca ggatctaaca
      421 ttcgcagctc gtcgctgtgg cctgatgcac ggcaaggtgc gataggctga gaatcgacct
      481 tgcaactgct atgctgcggt gcaacataat ttacgagtgc cgcacatcgc accttaagga
      541 caaccatgac atcatcaatg accggcgttc tggaagcggc gacccgcaac gccaatagca
      601 gcaacgacct cgctttggcc gccgcccaag tcatcgccag gcgcgccgcg cttggtctgg
      661 ctgccatctt tgacccaatg ggcgcggatc atgtcgaatt cggtcggatg gtgccggaga
      721 aggtggaagc gttctcggcg gcaggcatga tcatgatgcg gcacgccacg caggcgggcg
      781 aacagatcgc gcaattcgcg tctgacgctg tcatggccgc agtacgggcg acggttgcca
      841 tagtgggcag cgccagcccg gcggcaatgg cagaaacaca aggccagttc gccaagtcct
      901 ggtttgagca ggcggcgtcg aacttcatgg cgatcggcat tttagcgctg ggtgcgcagg
      961 aggcggcttt ggtcccaatt caagagacgc tcgcggccaa ttccgctcgg cttggcggat
     1021 agtcagccgc cgccgggtgc agtgacgcga tcggcgtcac atgcgcgcac agcatggcga
     1081 accgcccgcc tatcgctgcg ttcaagctgc tgtctggagc atgatcacgc ggggcggaac
     1141 gcctgaagcg cgtgaatcat gtgcccaaac aacgaggtag aacattttcg gcctgcagag
     1201 tccggctgga aatgctctag gcgagagatg ctctagatga gactggcaat aaaaataacg
     1261 gtcgggagag ttgtccatgg cttcgaatgt ggcggccact gtcgagccaa ttgaaaagga
     1321 aacgatgcgt cgcgtcgccc ggcgcctgat tccgcttctg atggctgcct actttgctgc
     1381 ctatctcgat cgtgtgaacg ttgggttcgc cgccctgacg atgaacaagt cgctggggct
     1441 gtccgccgag atcttcggca tcggcagcgg catcttcttt ctcggctact tcctgttcga
     1501 ggtccccagc aacctgatcc tgtccaaagt gggggcgagg cgctggatcg ggcgcatcct
     1561 gctgacctgg ggcgtgatct cggccgcgac agcattcatc accggcacct ggtccttcct
     1621 tggcatccga ttcctgttgg gccttgccga ggccggattt tatcccggca tcattctgta
     1681 tcttacctgg tggttccctt cgaattaccg ctcgcggatc atcggcatct tcatgaccgc
     1741 gatccccatc tccatcgtct cgggttcatt gatatccagc cagattctga ggtttggcgc
     1801 ctggggcggc ctggatggat ggcagtggct gttcatcctg gaagcgctgc ccgccatcgt
     1861 gcttggcgtc gtggtcatag tttacctgac ggacactccc gaacaggcgc aatggcttga
     1921 accggcgcag cgtgactggc tggtccgccg cctcggcgac gagcgcagcc agaaagaggc
     1981 ggttcacaac tactcgttcg ccgaaacggt tcgcaattcc cgggtctggc tgctgacgct
     2041 ggtctatttc ggccagaacg tcaccggcta cggcctggtt attttcttgc cacagatcgt
     2101 cagccggttt ggcgtcggcg tcggcatgaa cggcctgatc agcgcgcttc cctacctggc
     2161 tgccgcgatg gccatggtgt ggtggggcct gcatgcggat cggtcggccg agcatcgcct
     2221 gcacgccgcg gccgcctgct tcttgaactt cgccgggctg gcggcgtgcg tgttcctgga
     2281 gaatccgctc ttcatgatga ttgcgatcgt gctggggcag gtgggacagt ccgccatcgc
     2341 gccgaccttt tgggcgctgc cgaccgcgat gctgtccggc accgccgcgg ccggcgggat
     2401 cgcgttgatc aattcggtgg gaaatttggg gggattcctg ggtccataca tgatgggcag
     2461 cattcgggac gctaccggga gcttcaccat aggcctgttg tcgatcgcca ccggggcgtt
     2521 ggtcgcggcc atcgtgttgc tgctcctgcc gcgggagcag catctgcgac aagactccga
     2581 gagcaacgtg cgcgctgccg ccgattgagg caggcacgac ccctgaaggt tgccaagccg
     2641 ctgcgcgccg aggatgctcc tggcgcgcaa cgacaaggaa actgccgtga cacaaccgac
     2701 tggcccgctg cgcggcatcc gggttatcga catcactacc gtcgtgctag gcccatactg
     2761 ttcccaaacg ctgggcgaca tgggcgcgga cgtgatcaag gtcgaaacaa tcgagggcga
     2821 ctcgacgcgc ctgatcgggc cgtcccgcac gccgggcatg ggcagctact tcgccaacct
     2881 caaccgcaac aagcgcagcc tggcgatcga cctgaaaaag cccgaggcgc aagaggccat
     2941 tctgcgtctg gtcgagaccg ctgacgtatt tgttcacaac atgaggatcg gcgcggctca
     3001 gcggcttggc ctggactacg agagactcag cgcccgcaac ccgaagctga tctacgcttg
     3061 cgccagcggc ttccgcaagg gcagctcgat gcaggaattt ccggcctatg acgatcttat
     3121 ccagggggtt tccggcatcg cttcgttgaa cgccgggccg gacggcgcgc ctcgctactt
     3181 tcccacggtc gtggtggata aactgaccgg tgcgatgctc gcatcgatga tcgggatggc
     3241 cttgttccac cgagagagaa caggccaagg tcaggaaatt catctgccga tgatggagac
     3301 gatcctgtca ttctccctgg tcgagcatct ttggcacggc acgctgggcg aaccggagaa
     3361 gggcctgggc tatccccgca tgctgacgcc gcatcgccgc ccgtatgcca cgaaggacgg
     3421 ctttatcagt gtcatcgccc atagcgaccg gcaatgggcc aagctgtttc aggcgatggg
     3481 cgttccggaa ctgatcgagg atgtccggtt tgcttccgtg accgccagaa gcgccaacat
     3541 cgatgcggtg tacgccaccc tgacgaacgg gatgaggcag cgcaccaccg aagagtggct
     3601 ggcggagctt cgtccggcgg atattccctg cggcaaggcc aatagtctga acgacctgtt
     3661 cacagaccca tatcttgtcg aaaccggtta tttcgagccc catgtgcacc cttccgaagg
     3721 aagcgtggtg atacccgcca tcccggctca tttctctgga tcgcccgcga acgtgcatcg
     3781 cccatggccg aagctcggag aacacagtcg cgagatcctg gcagaggcgg ggttttcgga
     3841 cgctgagatc gagtcagtcc tttcccgcta acaaactccg atagccccgc acagcgtgcg
     3901 aaacactata atggcttcag cgcagcgggt ggaagcgtca gatgccgcca ttattgtggc
     3961 tgcctttgcc aattgtccga aggacttggt catcgcttga gccttccgtc gtcatagcgg
     4021 tgctcgcgac gccctggtgg ccgcagggcc acatcgacgg atttggccat acctgcaaaa
     4081 ggcgtggatg cggaccttcg tcggcatgac gaggcggtgc cgccacgaag tcgatctcgc
     4141 cgcgatctgg tattgccaga cgcggaagcc gcctgcttaa gccaaacatc catttgctgt
     4201 tgtaaacgct accctgaatg tcacgatgtt ggtgaggtat cgcagtcatc acagtcgcgt
     4261 tcgtatatcg atctcaatgc gacgtgattt cttttcttac tccctcatcg tcattgttat
     4321 gtacgcgcaa gccggtgcca aggagcggcg aagtacggct tcgtgaggac acaacagggc
     4381 tttgaaacga acatcagggc gcagcggaat ccgcttcagg cgcgataacg gaacggcaac
     4441 gctactatac tgctatttcg taatagtttc actgtccaat acaagggaaa ccagatggca
     4501 acttttagtt ggaacacggg cacgaacggt ttgtggaaaa tagccaccaa ctggacgcct
     4561 ggcacagtac cgaatgacat ccctcccacc gcgaccgtgg atgtgacgat cgatgccaag
     4621 gccaccaccg ccgcctacct cgtcaccatt gccgccaccg acacgatcac catcaattcg
     4681 ctgacgatga acgcggtgaa caatgcccag acccttaacc agacgccatt cctcggcgcg
     4741 atcctggagg ttgacgggac acttaagttc gcggcgggat cggccggtct gatcgatggg
     4801 ccgctgcaaa acgatcttgt cgtctccaac ggaaccatcg tgaacgccgg cacgatcaac
     4861 gccttcgtcc agggcaccgg agtcagtacc ttttccggca ataacgccat ctatttcacc
     4921 aactggctgc agtcgctcgg tacggtgatc gtggacaccg cgagcatcgc ggaattcaac
     4981 gccataacca acaccctgac cgacgggatc ttcgaggcca agggatcgac aaatgccgtg
     5041 gacttaggcg gcagtcttgg tcatctgata gtgaatatcg ggaccataca gggccccgga
     5101 atactgctgc caggcaccaa catcgagggc tggacccagt tgattttcga cggtccggat
     5161 tcgacgatca atgaatggaa cggcactacc tatgtgccgg tcgaatcaac gatccggctg
     5221 atccagaaca atgccacgat cagcgtgaat tccgatccca tctccaacct tggacgcgac
     5281 tacacgacgg ccaatgcact tacgatcggt gcgcaggcgc tgttcttcga gcaagccggc
     5341 acgc
//
LOCUS       DASWQN010000728         3214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_334600, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000728 DASWQN010000000
VERSION     DASWQN010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334600"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..744)
                     /locus_tag="VGC82_12390"
     CDS             complement(205..744)
                     /locus_tag="VGC82_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0424111.1"
                     /translation="MSDDVTGDVIDHGTRSRLRKAPNAFRTISEVADDLHIPQHVLRF
                     WETKFSQVKPLKRGGGRRYYRPDDIVLLRRIADLLYTQGYTIKGVQRLLREGGGLLSD
                     DIPPPTAEERAAAAAEKGDLPDKPPQIPGLDLVAPPASLMQLVRAAPRVRPDNETEQL
                     RQLLTEALQELEALRALLG"
     gene            complement(793..1104)
                     /locus_tag="VGC82_12395"
     CDS             complement(793..1104)
                     /locus_tag="VGC82_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY0424112.1"
                     /translation="MLTITRAHIAEMIYTQVGLSRNESADLLEDVLDRIATHLEHGES
                     VKISGFGTFSVRQKGRRIGRNPKTGEEVPILPRRVLVFRPSHVLKAHVNGIMPGTEDD
                     E"
     gene            complement(1223..2194)
                     /locus_tag="VGC82_12400"
     CDS             complement(1223..2194)
                     /locus_tag="VGC82_12400"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY0424113.1"
                     /translation="MPPRSILAGCGGYLPERIVSNDELSRSVDTSDEWIRERTGIRQR
                     HFAAKHETAAFMGTAASRAALADAGATAADVDAIILATSTPDQAFPATALRVQAALGV
                     TRGFGFDLSAACAGFIYGLSIADAMIRSGQARGVLVIGSEVYSRILNWQDRGTCVLFG
                     DGAGAVFVRANEDGDERHRGILSTHIHAQGTLGDILYVDGSVGQPDKPGHLVMNGREV
                     FRHAVVRLAEAVEEALAANGLARTDIDWLVPHQANKRIIDAMGRKLGLAPDRVVVTVD
                     RHANTSAASVPLALAEARADQRIRTGDLVLLEALGGGLTWGSALVRL"
     gene            complement(2207..>3214)
                     /gene="plsX"
                     /locus_tag="VGC82_12405"
     CDS             complement(2207..>3214)
                     /gene="plsX"
                     /locus_tag="VGC82_12405"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY0424114.1"
                     /translation="SMRLAVDAVASGEASGLVSAGNTGALLALAKIVIKSLPGIDRPA
                     MAAIGPSARGDVVMLDLGANVQCDVRNLVEFAVMGDVFARTVLGLTAPTIGLLNVGSE
                     ELKGDDTVREAAEILRASHIGPQFHGFVEGHDIAAGTTDVVVTDGFTGNVALKTGEGA
                     LKLIGEFLKQVFTNGIAGKIAYLLVRPGLDRLREWLDPRRYNGAVMVGLNGVVVKSHG
                     GTDAKGFAHAVDVAMDMVVHRFNDRIREDLARIALDKRETTAAEAKNAVPAGELPAEG
                     TVAKNLGAKDPGATDLGTKDLGTKDLGTKDLGTKDLVANDQRAGDPRPDSTDDTHLAT
                     A"
BASE COUNT          603 a         1053 c          975 g          583 t
ORIGIN      
        1 gcgtgtgaat gatgcgatca aaccagggca gagcatgatc acgcggggcg gtacgtcgga
       61 tgcgtgtgaa tcatgcaatg aaaccaaggc acagcatgat cacgcgaggc ggaacgtccg
      121 aagcgtgtaa atcatgcgat cagacaagag catttcccga ctgcacagtc aggctggaaa
      181 tgccctaatg ccgcgctggc tgacctaacc cagtagcgcg cgtagcgcct caagctcctg
      241 caatgcctcc gtcaacaact ggcgcaattg ctccgtctcg ttatctggcc tgacccgagg
      301 tgcggcacgg accaattgca tcagcgaggc cggtggggcg acaagatcca gtccggggat
      361 ttgcggcggc ttgtcgggca gatcgccctt ttccgccgcc gccgcggcac gctcctccgc
      421 ggtgggtgga gggatgtcgt cggacaacaa gccaccgccc tcgcgcaaca ggcgctgcac
      481 gcccttgatc gtatagcctt gagtgtaaag cagatccgcg atccgccgca gcaggacgat
      541 gtcatcgggc cggtagtacc gccggccacc gccgcgcttc aaaggcttta cctgggaaaa
      601 ctttgtctcc cagaagcgca gaacatgctg cgggatatgc agatcgtccg ctacttcgct
      661 gatcgtgcgg aaggcgttag gcgccttcct gagccgtgac cgggtcccat ggtcgatgac
      721 atcgccggtc acgtcgtcgc tcatgtgctg gtccaaggac cgccgcccgg ttcagtccca
      781 agctgggcga catcattcat catcttccgt accaggcatg atgccattga catgggcctt
      841 cagcacatga ctgggacgaa agacgagaac gcggcggggc aggatcggaa cctcttctcc
      901 ggttttcggg ttgcgcccga tacgccgccc cttctgtctt acggaaaagg tcccgaaccc
      961 gctgatttta accgattcac catgctccag gtgagtcgcg atccgatcca ggacgtcttc
     1021 aagcaggtcc gccgactcgt tcctggagag gccaacctgc gtgtagatca tttcggcgat
     1081 atgagcgcgc gtaatcgtca acatggccga acgctatgca gatcgaaccg gcacgtcaag
     1141 gttctcacga agaaaatgtt accggatcag gggtttgcgg ctgatcatgt cgcccgggtt
     1201 acaggcgggt cgtcattgga ggttataatc tgacgagggc cgatccccag gttaggccgc
     1261 cacccagcgc ttcgagcaat accagatcgc cggtgcggat gcgctgatcc gcgcgcgcct
     1321 cggccagcgc caacggaacc gacgcggcag acgtattggc atgacgatcc accgtcacca
     1381 cgacgcgatc cggtgccaac cccagcttgc gtcccatggc gtcgatgatc cgcttgttgg
     1441 cctggtgcgg caccagccag tcgatatcgg tccgagccaa gccgttcgcc gcgagggctt
     1501 cctcgaccgc ctccgccaag cggacaacgg cgtggcggaa tacctcgcgg ccgttcatca
     1561 ccagatgccc tggcttgtcc ggctggccga ccgaaccatc gacgtaaaga atatcgccca
     1621 acgtaccctg ggcatggatg tgtgtggaca agatgccgcg atgccgctcg tcgccgtcct
     1681 cgttcgcgcg gacaaagacc gccccggcgc catcgccgaa cagcacgcag gttccccggt
     1741 cctgccagtt caggatgcgc gaatacacct cgctgccgat gaccaggaca cccctcgcct
     1801 ggccggagcg gatcatggca tcggcgatgg acagcccgta aatgaacccg gcacaggcag
     1861 ccgacaggtc gaacccgaac ccacgtgtga cgcccagcgc cgcctgcacg cgcagcgccg
     1921 tcgcgggaaa cgcctggtcc ggggtgctgg tcgccaggat gatcgcatcc acgtccgcgg
     1981 cggtcgcgcc cgcatcggcc agggcagctc gtgaagcggc ggtgcccatg aacgcggcag
     2041 tctcgtgctt ggcggcaaag tgccgctggc ggattcccgt ccgctcccgg atccattcgt
     2101 cggaggtatc caccgaacgc gagagttcgt cattggagac gatccgctcg ggcaaatagc
     2161 ccccacagcc ggcgaggatg gaacgaggcg gcattgctgg tcctggtcag gcggtagcga
     2221 gatgagtgtc gtcagtcgag tccggccgcg gatcgccggc gcgctggtca ttggccacca
     2281 gatctttggt ccctagatct ttggtcccta gatctttggt ccctagatct ttggtcccta
     2341 gatctgtagc ccccggatct ttggccccca gatttttagc caccgtgcct tcagcgggca
     2401 attcgccggc aggaacagcg ttcttcgcct ccgcggcggt ggtttctcgt ttgtccagcg
     2461 cgatccgggc cagatcctca cgaatgcggt cgttgaaccg gtgcacgacc atgtccatgg
     2521 cgacatcgac cgcatgggcg aatcccttcg catcggttcc accatgcgat ttgaccacca
     2581 cgccgttcag gccaaccatc accgcgccat tgtaccgccg cggatcgagc cattcgcgca
     2641 atcgatcaag tcccggccgg accagcaggt aagcgatttt gcccgcgatg ccgttggtaa
     2701 agacctgctt gaggaattcg ccgatcagct tcagcgcacc ctctccggtt ttcagcgcga
     2761 cgttgccggt gaatccgtcc gtgaccacga catcggtggt gcccgcggcg atgtcatgac
     2821 cttcgacgaa gccatggaac tgcggaccaa tatggctggc ccgcaggatc tcggccgcct
     2881 cccgcacggt gtcgtcgccc ttcagttcct ccgacccgac gttcagcaat ccgattgtcg
     2941 gagccgtcag gcccagcacc gtgcgggcga acacgtcgcc catgacggcg aactcgacca
     3001 gattgcgcac gtcgcactgc acgttggcac ctaggtcgag catcacgacg tcgccccgag
     3061 ccgacgggcc gatagccgcc atggcggggc ggtcgatgcc tggaagcgac ttgatgacga
     3121 tcttcgccag cgccagcagc gcaccggtgt tgccggcgga caccagcccg gacgcctcgc
     3181 cggatgcgac ggcgtcgact gccaggcgca tcga
//
LOCUS       DASWQN010000729         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_598313, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000729 DASWQN010000000
VERSION     DASWQN010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598313"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VGC82_12410"
     CDS             <1..417
                     /locus_tag="VGC82_12410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY0424115.1"
                     /translation="CSPVRRQRMIDAFTPGAEGHALWAGLDVRPQDLLVRKRRFGAFA
                     PGASDLHEILQARGIDTLIITGTASQVCCESTARDAMMLNYKVFFVADGNATFTDEEH
                     NATLSAMAYTFCDVIETNALIGLIQVAADAREPAVA"
     gene            493..>1788
                     /locus_tag="VGC82_12415"
     CDS             493..>1788
                     /locus_tag="VGC82_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424116.1"
                     /translation="MIVSRRGLLASAAGAAAFPGVSAAAAAPQILRIGMTAADLPNTH
                     GIPNNGGEGFRFLGYPAYDSVVNWDFTHTDKLADIAPGLFTAWRIDEGNPLRWICDVR
                     QGVKFHDGTDFTADSVIFNLGRIYDDKSPQYDPPAAPIVRATVSMIDTFEKADDKTIV
                     ITTKYPFSFLPYLLTRILIVSPAQWEATGKNWAAFAKNPAGTGPFKITKVVPGQYAEM
                     SRNENYWDKARIPKLDKMIVYPMPEATTRVAALRSAQVDWIEVPPPDSIPSLKAAGLQ
                     ISLWPYPHTYPYCLNCTEGSPFKDERVRQAINYAIDREGMCAMLNGTAKPAIGLYPPD
                     DPMFGTPANRYKYDPEKAKALLAAAGYGPGKPMKAKIMISTSGSGQMVPIPMNEFMQQ
                     NFKAVGMDIDFDVVEWGTMLVAMRSDPKSPPSHGVDGINN"
BASE COUNT          353 a          568 c          547 g          320 t
ORIGIN      
        1 tgctccccgg ttcggcggca gcgtatgatc gacgccttca ctcccggcgc cgagggccat
       61 gccctgtggg ccggcctgga cgtcaggccg caggacctgc ttgtgcgcaa gcggcggttc
      121 ggtgcgttcg caccaggtgc atccgatctg catgaaatcc tgcaggcgcg cgggatcgac
      181 acgctgatca tcaccggaac cgcgtcgcag gtgtgctgcg aatcgacagc acgcgacgcg
      241 atgatgctga actacaaagt gttcttcgtc gccgacggca acgcgacgtt caccgatgag
      301 gaacacaatg ccacgctgtc ggcgatggcc tacacgttct gcgacgtgat cgaaaccaac
      361 gccctgatcg gcctgatcca ggtcgccgcg gacgctcggg agcccgccgt ggcataggcc
      421 ttgcagcgcg tcgctggtat ccgttgcacc cagtatccaa tgcacccggt atcaattgca
      481 caaggacacg gcatgattgt ttcgagacgt ggcctgttgg cctccgcggc cggcgccgcg
      541 gcatttcccg gagtttccgc ggccgcggcc gctccgcaga ttttacggat cggcatgacc
      601 gccgccgacc tgccgaacac ccacggcatt ccgaacaatg gcggcgaggg attccgcttc
      661 ctcggctacc ccgcctacga ttccgtggtg aactgggatt ttacccatac cgacaagctg
      721 gccgatatcg cgcccggcct gttcaccgcg tggcggatcg atgaaggcaa cccgttgcgg
      781 tggatctgcg atgttcgcca gggggtgaag ttccacgacg gtacggactt cacggccgac
      841 tcggtgattt tcaacctcgg ccggatttat gacgacaagt cgccgcaata cgacccaccg
      901 gccgcgccga tcgtgcgggc gacggtgtcg atgatcgaca cgttcgaaaa ggccgacgac
      961 aagacgatcg tcatcacgac gaagtatccg ttcagcttcc tgccgtactt gctgacccgt
     1021 atcctgatcg tcagcccggc gcagtgggag gcgaccggca agaactgggc agccttcgcg
     1081 aaaaatcccg ccggcacagg gcctttcaag atcaccaagg tggtgccggg gcaatacgcc
     1141 gagatgtcgc gcaacgaaaa ttactgggac aaggcgcgca ttccgaagct ggacaagatg
     1201 atcgtttatc cgatgccgga agcgaccacg cgtgtcgcgg ccctgcggtc ggctcaggtc
     1261 gattggatcg aggtgccgcc gccggattcc attccttcgt tgaaggcggc cggattgcag
     1321 atcagccttt ggccgtatcc gcacacctat ccctattgcc tgaattgcac cgagggctcg
     1381 cccttcaagg acgagcgggt gcgccaggca atcaactacg cgatcgaccg cgaaggcatg
     1441 tgcgcgatgc tgaatggcac cgccaagccc gccattgggc tgtacccgcc ggacgatccg
     1501 atgttcggca cgccggccaa ccgctacaaa tacgatccgg agaaagccaa ggccttgctg
     1561 gcggcggccg gctacggacc cggcaagccg atgaaggcga agatcatgat ctcgacctcg
     1621 gggtccggcc agatggtgcc gatcccgatg aacgaattca tgcagcagaa tttcaaggcc
     1681 gtcggcatgg acatcgactt cgatgtcgtc gagtggggaa ccatgctggt cgccatgcgc
     1741 agcgatccaa aatctccgcc gtcacatggc gtcgatggca tcaacaac
//
LOCUS       DASWQN010000730         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2445361, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000730 DASWQN010000000
VERSION     DASWQN010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2445361"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VGC82_12420"
     CDS             complement(<1..510)
                     /locus_tag="VGC82_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY0424117.1"
                     /translation="MAFLIMTVVGPDRPGLVSALSEAVAARNGSWLESRMARLAGQFA
                     GIVLVEAPEALLDDLRALESQGLQIVVQSGTSAAVETAAEPSSLLEVVGNDRPGIVRD
                     ITQILVRNRINIEELTTGVASGSFSGETLFRVTARVRAPDPAAMEAMRAGLELLGNEL
                     MVDIHPAPEG"
     gene            703..1881
                     /locus_tag="VGC82_12425"
     CDS             703..1881
                     /locus_tag="VGC82_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0424118.1"
                     /translation="MSNFVSGRHFLQTPGPTNLPDRVLRAMDRNAINHRGPEFGALGR
                     EIIAKLRGVFQAASATIGIFPASGTGAWESALANTLTAGDRILISRTGQFSHLWEQLA
                     ARFGLDVIPLETDWRRGGDPVAIGEMLAQDREHKIKAVCVVHSETSTSCMTDVLAVRQ
                     AMNRTSHPALLMVDAISSLACADYRHDEWEVDVTIAGSQKGLMVPPGLSFTVISDKAM
                     AVARAGGSRRSYWDWEPLVTSNKTGFFPYTPAINLLYALNEALDMLAEEGLPNVFARH
                     NRYAQATRLAVAAWGLELQCADPTAYAPGVTAIRTPEGHSADRLRNIILEKFNMSLGN
                     GLGRIENRVFRIGHMGDLGVLSLTGTLTGVEMGLLAAGIPHNQGGVQAAMNYLAASNG
                     "
BASE COUNT          345 a          689 c          632 g          355 t
ORIGIN      
        1 gccttcgggc gcgggatgga tgtccaccat cagctcgttg ccgagcagct ccagccctgc
       61 ccgcatcgcc tccatcgccg ccgggtccgg cgcgcgcacc cgggccgtta cccggaacag
      121 cgtctcgccg ctgaacgacc cgctcgcgac acccgtggtc agttcttcga tattgatcct
      181 gttccgaacc aggatttggg tgatgtcgcg cacgattcct ggccggtcgt tgccgacgac
      241 ctctagcagc gaactcggct ccgccgctgt ttcgacggcg gcggacgttc cgctctgcac
      301 cacgatctgc aggccctggc tttccaatgc ccgcagatcg tccagcagtg cctccggcgc
      361 ctccaccagc acaatgccgg cgaactgccc ggccagccga gccatccggc tttccagcca
      421 actgccgttg cgggcggcga cagcctcgga caacgcactg acgagacccg ggcggtccgg
      481 cccgacgaca gtcatgatga gaaaagccat ggccgatggt cctgaacctg ttcagcgcga
      541 tcaaatactg cctccggcga catcgagcca gaccatgtcc attccccggc gggtggcgtc
      601 gaacagactg tcgcgccgcc acgcacccca cctattcgcc aacgttattg caaaccggga
      661 cgcgacatgg ttgttacgcg cccattggag ggtgcgttcc cgatgtcgaa ctttgtttcc
      721 ggccgccatt tcttgcagac ccccgggccg accaatctgc ccgaccgggt gctgcgcgcc
      781 atggaccgca atgcgatcaa ccaccggggc ccggaattcg gcgcgctggg ccgggaaatc
      841 atcgccaagc tacgcggcgt gttccaggcc gcctcggcca cgatcgggat ttttcctgcc
      901 tccggcaccg gtgcgtggga atcggcgttg gccaacacgc tgaccgccgg cgaccgcatc
      961 ctgatcagcc gcaccggcca gttctcgcat ctgtgggaac aattggcggc gcgcttcggc
     1021 ctggacgtga tcccgctgga gactgattgg cgccgtggtg gcgatcccgt cgcgatcggc
     1081 gagatgctcg cgcaggatcg tgagcataag atcaaggcgg tttgcgtggt tcacagcgag
     1141 acctcgacaa gctgcatgac ggacgttttg gcggtgcggc aggcaatgaa ccggacctct
     1201 catccggcgc tgctgatggt cgatgcgatc tcctcgttgg cctgcgccga ttaccggcac
     1261 gacgagtggg aggtcgatgt gacaatcgcc ggctctcaga aagggctgat ggtgccgccg
     1321 gggctgtcgt tcaccgtgat cagcgacaag gccatggcgg tggcacgcgc cggcggatcg
     1381 cgccgcagct attgggactg ggagccgttg gtcacctcca acaagaccgg tttcttcccc
     1441 tacactccag cgatcaatct tctctacgcg ctgaacgagg cactggacat gctggccgag
     1501 gaaggcctgc cgaacgtctt cgcccgccac aaccgctatg cccaggccac gcgtctggcc
     1561 gtcgccgctt ggggtctgga actgcaatgc gccgacccga ccgcctacgc gcctggggtg
     1621 accgcgatcc gcacgccgga ggggcacagc gccgacaggc tgcgcaacat catcctggaa
     1681 aagttcaaca tgagcctggg caacgggctt ggccggatcg agaaccgcgt gttccgcatc
     1741 ggccacatgg gcgacctggg cgtgctgtcg ctgactggca cgctaaccgg ggtggagatg
     1801 ggacttctcg cggccggcat tccgcacaat caaggcggcg tgcaggcggc gatgaactat
     1861 ctggcggcgt cgaacggctg aggacctctc gcggccgaca tcagcccggc atcacctgga
     1921 tcgctttgta aagccgcctc gacagccttg cgattatctc ctggacgttg ccaacgttaa
     1981 gcagcgttgc cgcgccggca tagaccgccg ctccgagccc g
//
LOCUS       DASWQN010000731         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2445369, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000731 DASWQN010000000
VERSION     DASWQN010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2445369"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..1428)
                     /locus_tag="VGC82_12430"
     CDS             complement(346..1428)
                     /locus_tag="VGC82_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0424119.1"
                     /translation="MSEGQTKTAFGRIFPPRDAWLAKAPPEPIIDPDLPIIDTHHHLW
                     HRTGGARGESWEVPAFRYLIDEFLADCATGHNVVGSVFLQCHSMYRATGPVQMRPVGE
                     TEFVAGIAAMSASGGYGKTAVAAGIVGYADLTQGDWVTPVLEAQLRAGGGRFRGVRHS
                     AGYDADPVIGNSAPDMQPGLYLRPDFRAGMGRLAALGLSLDAWGFHPQMPDIVDLARA
                     FPGSNIIVGHCGGPLGYGRYAGQRDEVFADWKRSVAELAKCQNVTIKLGGMMMRLAAY
                     DYMALDAPPSSEQLAGHWRPYVETCIELFGANRCIAESNFPVEKMGIGFAALFNALKR
                     MAGGCSGDEKAAIFAGTARRVYRLDC"
     gene            complement(1493..>2216)
                     /locus_tag="VGC82_12435"
     CDS             complement(1493..>2216)
                     /locus_tag="VGC82_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424120.1"
                     /translation="SWWTLLPNRVDLWAESSRKRREDLFGAATKESLMDRYSRSATLA
                     CLAAMAVALTPIAAVAQMRTYYHVGAWDAFSGRSDSGGAVCGVGTTNRTEQRSLSIRF
                     DIGGSETMFAASKPDWSIPQDTRVTVVMQIGLDTPWTQQASGNGHSINWALDRNAMQV
                     FDRQFRGAASMTVTFPEGNEPPWTLSLAGSTAISDTFARCVRDLTRQVQATQPRADDA
                     PPQGPTQPFSPPAVAAPALPPH"
BASE COUNT          415 a          750 c          659 g          392 t
ORIGIN      
        1 tcatcgactt gttgccgaat aatatctatc gctcgactac agtattatcg aaaccgctca
       61 cactacaagg cacacggttt atcaaaacga ggagtaccgt tgccgcaagc cggaatgaat
      121 ctaggcgcag acgatatgaa accgcgccac aggccccgcg aaatcctttg acggcgactt
      181 tggattcacc catgacccaa tgagcaagcc tccggcgccc tcaaccatgc aatcaacaaa
      241 gtgttgatct aatcagccgt tccactgttc caccagtatt cgttcttcac ttgaagcagt
      301 catcgcggcc gaacgaaggc ttggccgcca aaccacggcc acgcttcagc aatccaaacg
      361 ataaacccgt ctggccgtgc cggcaaagat agcggccttc tcgtcgccgg agcatccgcc
      421 cgccatccgc ttcagcgcgt tgaacagggc cgcgaagcca atccccattt tctccaccgg
      481 gaagttgctc tcggcgatgc agcggttcgc accgaacaat tcgatacagg tctcgacata
      541 cgggcgccaa tggccggcga gttgttccga cgatggcggt gcgtcgagcg ccatgtagtc
      601 ataagccgcc agtcgcatca tcatcccacc gagcttgatc gtcacattct ggcatttcgc
      661 cagttcggcg accgaccgtt tccagtcagc gaagacctcg tcccgctggc cggcgtaacg
      721 gccatacccc agcgggccgc cgcaatgtcc gacaatgatg ttgctgccgg ggaaggcacg
      781 agcaaggtca acgatgtcag gcatttgcgg gtgaaatccc caggcatcca gcgacaggcc
      841 cagtgcggca agccggccca tcccggcgcg gaagtcggga cgcagataaa gaccgggctg
      901 catgtccggc gcgctgttgc cgatcaccgg gtcggcatcg tatccggccg agtgccgcac
      961 gccgcgaaac cgcccgccac cggctcgcag ctgtgcctcc agcaccggcg taacccagtc
     1021 gccttgcgtc agatcggcat atccgacaat gcccgcggcc accgcggtct tgccgtagcc
     1081 gccgctggcg ctcatggcgg cgatacccgc gacgaactcg gtctcgccta ccggacgcat
     1141 ctggaccggt ccggtcgcgc ggtacatcga atggcactgc aggaatacgc tgccgacgac
     1201 attgtgaccg gtcgcgcagt ccgccaggaa ctcgtcgatc aggtagcgga acgccggcac
     1261 ctcccacgat tcgccgcgcg ccccgccagt gcggtgccac aggtggtgat gggtgtcgat
     1321 gatcggaaga tccggatcga tgatcggctc cggcggcgct ttcgccagcc aggcatccct
     1381 tggcggaaat atcctgccaa aggcggtctt ggtttgtccc tcgctcatcg gcgcttcctc
     1441 aatctgcttg ccgcgattac cgcacggtct cgcgccatgc tcaagccggc ggtcagtgcg
     1501 gtggcagtgc cggggccgcg acggctgggg gactgaaagg ctgcgtcggc ccttgaggcg
     1561 gcgcgtcgtc cgcccgcggc tgagtcgcct gcacctggcg ggtcaggtcc ctgacgcagc
     1621 gggcaaatgt gtcgctgatg gcggtggaac ctgccagcga cagcgtccac ggcggttcat
     1681 tgccctccgg gaaggtcacg gtcatcgacg ccgcgccgcg gaactgccgg tcgaagacct
     1741 gcatcgcgtt gcggtccagc gcccagttga tggaatgtcc gttgccgctg gcctgctgcg
     1801 tccagggtgt atcaaggcca atctgcatca ccaccgtcac gcgggtgtcc tgcgggatcg
     1861 accagtccgg cttgctggcc gcgaacatcg tctccgaacc gccgatatcg aaccggatcg
     1921 acaggctgcg ctgctcggtc cggttcgtgg tgccgacgcc gcaaaccgcg ccgcccgagt
     1981 cgcttctgcc gctgaacgcg tcccaggcgc cgacatgata gtaggtccgc atctgcgcca
     2041 cggcggcaat cggggtcagc gcgaccgcca tcgccgccag gcaggccagc gtggctgatc
     2101 gagaataacg gtccatcagg ctttccttcg tcgctgctcc gaatagatcc tcgcgccgct
     2161 tccgggacga ttccgcccac aaatccacac ggttgggcag caatgtccac cacgaa
//
LOCUS       DASWQN010000732         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1125557, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000732 DASWQN010000000
VERSION     DASWQN010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125557"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1948
                     /locus_tag="VGC82_12440"
     CDS             <1..1948
                     /locus_tag="VGC82_12440"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0424121.1"
                     /translation="LYEGHAIAAVAAISSSIADQAAALIKVDYEVLPHVIDVDEAMAP
                     DAPLLFEDMITRGVEPAPTKPSNISKRLEFAIGDVEAGFKMADVVIEKEFKTAAVHQA
                     YIEPHACVAKYEADGQCEIWSSSQGHFQMRALTAQILGAKLGDLRVTPAEIGGGFGGK
                     TVTYLEPVAATLSRKSGAPVRLQMSREDVFRASGPTSGASMWVKIGVKKDGTIVAADG
                     IFKFQAGAFPGSPVMNGCLCAYAPYEIANQRAVGYDVVSNRPKAAAYRAPGSPISAFA
                     VESVLDMCAKAIGMDPLEIRRKNACHIGTPTLAGPKHAHAGYVETVEALMNHPAYKVK
                     LGPNQGRGVASGYWFNGGGESSATLQVNADGTVLVATGSPDVGGSRASMAIMAAETLG
                     VDYNTVRAIVADTASVGYTHVTGGSRVTFATGMAVVNTSKTVVKELCKRAALIWGVDE
                     EGVIWEDGHAKPAGSNVGDFKPLSLKEIAAKAAATGGPITASGGVNAGGQAPGFSTQF
                     CDVEVDPETGRVTILRFVAAQDVGRAIHPSYVEGQIQGGVVQGIGWALNEEYIYNANG
                     GLDNPGFLDYRCPVASDLPMIDAIMIEVPNPRHPFGAKGVGEVPIVPPLAAVANAVRA
                     ALGERMYDLPLSPPKIRAVIDAKG"
BASE COUNT          359 a          606 c          721 g          314 t
ORIGIN      
        1 cctgtatgaa ggacatgcca tcgctgcggt tgccgcgatc agcagcagca tcgccgacca
       61 ggcagcggcg ctgatcaagg tggactacga ggtcctgccg catgtcatcg acgtggacga
      121 agcaatggcc cccgacgcgc cgttgctgtt cgaggacatg atcacgcgcg gcgtcgagcc
      181 ggcgccgaca aagccgtcca atatctccaa gcggttggaa ttcgcgatcg gcgacgtcga
      241 ggccggcttc aagatggctg atgtggtgat cgagaaggag ttcaagactg ccgccgtcca
      301 ccaggcgtat atcgagccgc atgcctgcgt cgcgaagtat gaggcggacg ggcagtgcga
      361 aatctggtcc tccagccagg gccacttcca gatgcgcgcg ctgacggcgc agatcctggg
      421 cgcgaaactg ggtgacctgc gggtaacgcc ggcggaaatc ggcggtgggt tcggcggcaa
      481 gacggtcacc tatctggaac cggtggcagc cacgctgtcg aggaaatccg gcgccccggt
      541 gcggctgcaa atgagccgcg aggatgtatt ccgcgcttct ggcccgacct cgggcgcgtc
      601 gatgtgggtg aagatcggcg tcaagaagga cggcacgatc gttgccgccg atggcatctt
      661 caagttccag gcgggcgcct tcccgggttc gccagtgatg aacggttgcc tgtgcgctta
      721 cgcgccgtac gagatcgcca accagcgggc ggttggctac gatgtcgtgt cgaaccggcc
      781 gaaggcggcg gcgtatcgcg cgcccggctc gccgatctcg gccttcgcgg tcgaaagcgt
      841 gctggacatg tgcgccaagg cgatcggcat ggacccgctg gagataaggc gaaagaatgc
      901 ctgccatatc ggcacgccga cgctggccgg gccgaagcac gcccatgccg gatacgtcga
      961 aacggtcgag gcgctgatga accacccggc ctacaaggtg aaactgggtc cgaaccaggg
     1021 cagaggagtc gcctccggct actggttcaa cggcggcggc gaatccagtg ccacgctgca
     1081 ggtgaatgcc gatggcaccg tgctggtggc gaccggcagc ccggacgtcg gcgggtcgcg
     1141 tgcttcgatg gcgatcatgg cggcggagac gctgggggtc gattacaaca ccgtccgggc
     1201 aatcgtggcg gacacggcgt ccgttggcta cacgcatgtc accggtggct cgcgggtgac
     1261 gttcgcgacc ggaatggcgg tggtgaacac ctccaagacg gtggtgaagg agctgtgcaa
     1321 gcgcgccgcg ctgatctggg gcgtggacga agagggcgtg atctgggaag acgggcacgc
     1381 caagccggcc gggtcgaacg tcggtgactt caagccgctg tcgttgaagg aaatcgcggc
     1441 gaaggcggcg gcgacgggcg gcccaatcac cgcatccggc ggcgtgaatg ccggcgggca
     1501 ggcgcccggt ttctccaccc agttctgcga tgttgaagtc gatccggaga ccggccgggt
     1561 gacgatattg cgcttcgttg ccgcgcagga tgtcgggcgg gcgatccacc caagctacgt
     1621 ggagggccag atccagggcg gcgtggtgca gggcatcggc tgggcgctga acgaagaata
     1681 catctacaac gcgaacggcg gcctcgataa tccgggcttc ctggactacc gctgtccggt
     1741 ggcctcggac ctgccgatga tcgacgccat catgatcgag gtgcccaacc cacggcaccc
     1801 gttcggggcc aagggcgtgg gcgaggtgcc gatcgtgccg ccgctggccg cggtggccaa
     1861 cgcggtccgg gcggcgctgg gcgagcggat gtacgacctg ccgctgtcgc cgccgaagat
     1921 ccgcgcggtg atcgacgcca agggctgacg gacacgtcat gccccacgtc gtcctcgcca
     1981 gctcgaacgg tcgggaattc
//
LOCUS       DASWQN010000733         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1125629, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000733 DASWQN010000000
VERSION     DASWQN010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125629"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VGC82_12445"
     CDS             complement(<1..866)
                     /locus_tag="VGC82_12445"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0424122.1"
                     /translation="MTVKAQAMDPYPDFLDSTNRLADSHTLRTRLDRDGYLFIRNLLP
                     AQTILAVRERLLAKAATGGWLHPDSPVQAGIANPAAACKDPEDRYMRVFRGLWMDEEL
                     HRLRIHPVVTDLFTRIFGEPALAHPMFVQRNIFPQAGSFDFTTGMHQDRVHIGGATSY
                     ALWMPLGDCPREKGALAVAAGSHTKGILETRVGSGAGGMDIAVPVPGKWVTGAFRAGD
                     ALIFQDVTVHKALPNRTREIRMSFDARFQPVSQPVADTNMVPYAGCGTWEDVYAGWEW
                     TDGQYFWKDLTL"
     gene            1057..>1708
                     /locus_tag="VGC82_12450"
     CDS             1057..>1708
                     /locus_tag="VGC82_12450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424123.1"
                     /translation="MSGNSRTAPNIPWWKEPTKDQWYAYCAAWMGWTLDAFDFTVFLL
                     IMAPIAQEFGVPLIDVTAVFSVTLIMRLGGATASGWLADRMGRRAPLMISILWYSVCN
                     FAAGFSPSFTFLFVVRALLGIGMGAEWPAGAALAMESWPARSRGLMSGVLQGSWGLGF
                     ALSALAYGFLYGPLEAWHKGYGWRGMLILGVLPALACVWIRFYVKEPEVWTENKKIQ"
BASE COUNT          298 a          513 c          556 g          341 t
ORIGIN      
        1 tcgagcgtca ggtccttcca gaaatactgg ccatcggtcc attcccatcc ggcataaacg
       61 tcctcccagg tgccgcagcc cgcataggga accatgttgg tgtcggcaac gggctggctc
      121 accggctgga agcgcgcgtc gaacgacatg cggatttccc gggtccggtt cggcagcgcc
      181 ttgtgcaccg tgacgtcctg gaagatcagc gcgtcgcccg ccctgaacgc cccggtcacc
      241 catttgccgg ggaccggcac ggcgatgtcc atcccaccag cacccgagcc gaccctggtt
      301 tccaggatac ccttcgtgtg cgaaccggcc gcaacggcca gcgcgccttt ttcgcgcggg
      361 caatcgccga gcggcatcca cagcgcatag cttgtggcac cgccgatatg gacgcgatct
      421 tgatgcatgc cggtggtgaa gtcgaagctg ccggcctgcg ggaagatgtt gcgctgtacg
      481 aacatcggat gagccagcgc cggttcgccg aagatccggg tgaacaggtc ggtgacaacg
      541 ggatggatgc gtagtcgatg cagttcctca tccatccaga ggccacggaa cacgcgcatg
      601 tagcgatcct cgggatcctt gcacgcggcc gctgggttgg cgatgccggc ctgcaccggg
      661 ctgtcggggt gaagccagcc gcctgtcgcc gccttggcca gcaggcgttc gcgcacggcg
      721 agaattgtct gcgccgggag aaggttgcgg atgaacaggt agccgtcacg atccagccgg
      781 gtacggagcg tatgactgtc ggctagccga ttggtcgagt cgagaaagtc gggataggga
      841 tccatcgcct gtgccttcac cgtcatggac aggctcgaac tatgcaggcc ggtcgccctc
      901 cacgcaatcg acaccggcga cgtgccgaaa tcgcacctcc tgcgtcgact ccgatgtgaa
      961 ttgcggatgg ctactatacg cgcctacgct tcctgtgaga tgagtcaagc gactgctcgt
     1021 cccgaacaca attacaataa acgtgggaac aaaacaatgt ctgggaatag ccgaaccgca
     1081 ccgaatatcc cttggtggaa agagcccacc aaggaccagt ggtatgccta ttgcgccgcc
     1141 tggatgggct ggacgctgga tgccttcgac ttcaccgtct tccttctcat catggcgccg
     1201 attgcgcagg agttcggggt cccgctcatc gatgtaacgg cggttttttc ggtcaccttg
     1261 atcatgcgcc tgggcggcgc gaccgcctcc ggttggttgg ccgatcgcat ggggcgccga
     1321 gcgccgctga tgatctccat tctttggtac tcggtctgta atttcgccgc cgggttctcg
     1381 cctagcttca ccttcctgtt cgtggttcgg gcgctgttgg gaatcgggat gggggccgaa
     1441 tggccagccg gagcggcgct ggcgatggaa tcgtggccgg cgcggtcgcg cggtctgatg
     1501 tccggcgtgt tgcaggggtc gtgggggctg ggcttcgcgt tgtcagcgct cgcttacggc
     1561 ttcctgtacg ggcccctcga agcgtggcac aagggttacg gttggcgcgg catgctgatc
     1621 ctgggcgtgc tgccggcttt ggcctgcgtc tggatccgct tttatgtaaa agagccggaa
     1681 gtctggacag aaaacaagaa gatacagg
//
LOCUS       DASWQN010000734         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_466520, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000734 DASWQN010000000
VERSION     DASWQN010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466520"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VGC82_12455"
     CDS             <1..286
                     /locus_tag="VGC82_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0424124.1"
                     /translation="VEQLARNHEVLIIIEEAAIGGFGARVMHHLAWKGLLDSGLKIRP
                     MVMPDLFIDHDSQPKQLAEAGLSAKDMVAVALGAVGIEVPRVGSKVVTSR"
     gene            432..1037
                     /locus_tag="VGC82_12460"
     CDS             432..1037
                     /locus_tag="VGC82_12460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424125.1"
                     /translation="MLLSAVACAVASARGARAQPDASPDQPLRELYAALEATMRSGNA
                     APFQKRFDALAPFVDRAFDLETVLKVSIGLRWDNMDANLRDRLLKAFRRFTIATYVAN
                     FDKYDGERFRILPGTRESGSDRIVETEIVNGGQRIRLDYVMREAGGAWRIVDVLLDGS
                     ISRVAVQRSDFRKIMATGDADALIRSLHRKVADLSNGALSS"
     gene            1034..2248
                     /gene="hpnI"
                     /locus_tag="VGC82_12465"
     CDS             1034..2248
                     /gene="hpnI"
                     /locus_tag="VGC82_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HEY0424126.1"
                     /translation="MIAVLAGAAAVLAAIGVIQSLVASRLVAAFAGRPIDRPASRPAV
                     TILKPLHGDEPLLEAALTTTCRQDYPNWQIVFGVQHQADPAAAVVRRLQAAFPLVDIA
                     LVVNPALHGANRKIGNLINMLPAARHDVLVIADSDLHVRPDYLDRLIAALQQPNIGLV
                     TTLYAGLPAVSKLPARLGATQITHGFLPGALLSRALGRQDCLGATMCLRRHDLARIGG
                     FPALVGHLADDNVLGRRVAALGLRVALAATVPLTTVPEATVRALFRHELRWARTIRAL
                     EPAGFAASVLQYPLAWALLTVLLSGAALWSIALFLVAWVLRAVAALGVDRALAPLWVE
                     EHGSDDAVALAFSCPVWLLPLRDLLSVAVMLASYGGRHVEWRGHGLHADTPPPVCRNS
                     EALRPIEGTNAR"
     gene            2245..>3480
                     /gene="hpnJ"
                     /locus_tag="VGC82_12470"
     CDS             2245..>3480
                     /gene="hpnJ"
                     /locus_tag="VGC82_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HEY0424127.1"
                     /translation="MMKTLFLHPPSFDGFDGGAGARYQAKREIKSFWFPTWLAQPAAL
                     VPGSKLIDAPPARIGLEAVTRQARDYELCVMHTSTPSFASDVKVAEAMKAANPSLKIG
                     FVGAKVAVQPAESLAQGGAIDFVARNEFDFTIKEIAEGRDWGSVDGISYRNKAGVIIH
                     NKDRQILEDMDQLPFVTEVYKRDLRIEDYFIGYLMHPYVSLYTGRGCKSHCTFCLWPQ
                     TVGGHRYRVRSPGHVAEEIRQAKAMFPQVKEFFFDDDTFTDNLPRAEAIAKELGKMGV
                     TWSCNAKANVPRETLKVLKDNGLRLLLVGYESGNQQILYNIKKGMRIEVARKFTKDCH
                     ELGVKIHGTFILGLPGETKETIEETIRFATDINPHTIQVSLAAPYPGTFLYKQAVEHG
                     WLDAEHAELVDTSGVQIAPL"
BASE COUNT          579 a         1058 c         1168 g          675 t
ORIGIN      
        1 ggtcgagcaa ctggcgcgga accacgaggt cctgatcatc atcgaggaag ccgcgatcgg
       61 cgggttcggt gctcgggtga tgcaccatct ggcttggaaa gggttactcg attcgggatt
      121 gaagatccgt ccgatggtca tgccggactt gttcatcgac cacgattccc agcccaagca
      181 gttggcagag gccggccttt ccgcgaagga catggtcgcc gtggcgctgg gggccgttgg
      241 aatcgaggtg ccccgggtag ggtcgaaggt cgtcacctcg cgatgattgg gctgttggca
      301 ggggatgcgt ttttcggctt ggcggatcgg cgtgccggca ccggtccttt tgaattgagc
      361 cgcgcctgac catgacggac gggactgttc caagatcagc cgcggggccg gctgtcccga
      421 gccgccgcgt tttgctgctg tccgccgtgg cctgtgcggt cgcgtcggcg cggggtgccc
      481 gggcacagcc ggacgcatcc cccgatcagc cgctgcggga gctttacgcc gcactggaag
      541 cgacgatgcg gtccggcaac gcggcgccgt ttcagaaaag attcgatgcg ctggcgcctt
      601 tcgtggaccg ggccttcgac ctcgagaccg tgctgaaggt ttcgatcggt ctgcgctggg
      661 acaacatgga tgccaatctc cgcgaccgcc tgctgaaggc atttcgccgc ttcaccatcg
      721 caacctatgt ggccaacttc gacaaatacg acggcgagcg ctttcgcatc ctgccaggaa
      781 cgcgcgagtc cggatccgac cggattgtcg agacggaaat cgtcaacggc ggccagcgca
      841 ttcgcctgga ctacgtgatg cgggaggccg gtggcgcctg gcgtatcgtg gatgtgctgc
      901 tggacgggtc gattagccgg gttgccgttc agcgttcgga ctttcgcaag atcatggcca
      961 ccggcgacgc ggatgccctg atccgaagcc tgcaccgcaa ggtcgccgat ctttcgaatg
     1021 gtgcgctgag ttcatgatag ctgttctggc cggtgcggcc gcggtgctgg ccgcgattgg
     1081 tgtgatccag tctttggttg cttcgcgcct ggtcgcggcg ttcgccggcc ggccgatcga
     1141 tcgaccggct tcgcggccgg cggtgacgat tttgaagccg ttgcacggcg atgagccgct
     1201 gctggaggca gctctgacga caacctgccg gcaggactac ccgaactggc agatcgtgtt
     1261 tggcgtgcag catcaggccg atccggcggc tgcggtggtg cggcgcctgc aggcggcatt
     1321 tccgctggta gacatcgccc tggtggtcaa tcccgcgctg cacggcgcca accgcaaaat
     1381 cggcaacctg atcaacatgc tgcccgccgc ccggcatgat gtgctggtga tcgccgattc
     1441 cgatctgcat gtccggccgg attatctcga ccgcctgatc gcggccctgc agcaaccgaa
     1501 tattggtctg gtcaccacgc tttatgccgg ccttccggca gtttccaagc tgcccgctcg
     1561 gctgggtgcc acacagatca cgcatggatt cctgccaggc gcgctgctgt cgcgggcgct
     1621 tggccggcag gattgcctgg gggcgacgat gtgcctgcga cggcatgacc tggcgcgtat
     1681 cggcggcttc ccggcgctgg tcggtcacct cgcggacgac aatgtcctcg ggcgccgggt
     1741 tgccgccctg gggctgcggg tcgcattggc cgcgaccgtg ccgctgacga cggtgcccga
     1801 agcgactgtc cgggcactgt tccggcacga actgcgctgg gcgcggacga taagagctct
     1861 ggagcccgct ggatttgccg cttcggtgct gcaataccct ctggcttggg cgctgctgac
     1921 cgtcctgctg agcggcgcgg cgctatggtc gatcgcgctg tttctggtcg cctgggtatt
     1981 gcgggccgtc gccgctttag gtgttgaccg ggcattggcg ccgctatggg tcgaagaaca
     2041 tggcagcgac gatgccgtcg cacttgcttt ctcctgcccg gtctggcttt tgccgctgcg
     2101 agacctgtta tcggtggccg tgatgctggc cagctatggc ggccgccacg tggaatggcg
     2161 cggtcatggc ttgcacgccg atacgccgcc gcctgtttgc cgcaactccg aagcactccg
     2221 cccgatcgag ggaaccaacg cgcgatgatg aagacactgt tcctgcaccc gccttcgttc
     2281 gatggtttcg acggtggtgc cggtgcgcgc taccaggcga agcgcgagat caagtcgttt
     2341 tggttcccga catggctggc tcagccggcc gctctggtgc ccggcagcaa gctgatcgac
     2401 gcgccgccgg ccaggatcgg gctggaggcg gtgacacggc aggcgcgcga ttacgagcta
     2461 tgcgtgatgc acacctccac accgtccttc gcgtcggatg tgaaggtcgc cgaggcgatg
     2521 aaggcggcca atccatcgct caagatcggc ttcgtcggtg ccaaagtcgc cgtgcagccg
     2581 gccgaaagcc tggcccaggg cggcgccatc gacttcgtcg cgcgcaacga gttcgatttc
     2641 acaatcaagg agatcgccga gggccgcgac tggggttcgg tggatggcat ttcctaccgc
     2701 aacaaggccg gcgtcatcat ccacaacaag gatcggcaga tcctcgagga catggaccag
     2761 cttccgtttg tcaccgaagt gtacaagcgc gacctgcgga tcgaggacta cttcatcggc
     2821 tacctgatgc atccctatgt ctcgctctat accgggcgcg gctgcaagtc gcactgcacg
     2881 ttctgcctgt ggccgcagac ggttggcgga catcgctacc gggtgcgctc tccggggcat
     2941 gtggctgagg aaatccgcca ggcgaaggca atgtttccgc aggtgaagga gttcttcttc
     3001 gacgacgata ctttcaccga taatctgccg cgtgccgagg cgatcgcgaa agagctcggc
     3061 aagatgggcg tgacgtggtc ttgcaacgcc aaggcgaacg tgccgcgtga gactttgaag
     3121 gtactgaagg acaacgggct gcggttgctg ctggtgggat acgagagcgg caaccagcag
     3181 atcctctaca atatcaagaa gggcatgcgg atcgaggtcg ccaggaagtt caccaaggac
     3241 tgtcacgaac tgggggtgaa gattcacggg acgtttatcc ttggcctgcc gggcgagacg
     3301 aaagaaacga tcgaagaaac catccgcttt gccacggaca tcaacccgca cacgatccag
     3361 gtgtctctgg ccgcgccgta tccgggcacg ttcctgtaca agcaggcagt ggagcacggc
     3421 tggctggatg cggagcacgc cgaactggtc gataccagcg gcgtgcagat tgccccgctg
//
LOCUS       DASWQN010000735         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_203010, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000735 DASWQN010000000
VERSION     DASWQN010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203010"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1783
                     /locus_tag="VGC82_12475"
     CDS             <1..1783
                     /locus_tag="VGC82_12475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0424128.1"
                     /translation="GQPNRTGIREIDDLGWQLYRAAEERARAQSAMAYQITLLRAVTE
                     STADAIFMVDPQGLVTYANPESARMFGWNEDELIGQTLHHVADGRRTSGSANAAWDSK
                     LTRTVTAGRASVREEDIFFRRDGQMVMVECTYSPVVVDDAPAGAVLTVRDISARKRID
                     AALRENEARLCELVSTLDLAKILVRDPNGTIRFWSFGCERLYGWSGAEAIGRSTFSLL
                     QTVLPVPQDEINAVLLRDGEWSGDLIQTCRDGSLITVAVRSVLQRGAQNEPRAIMESV
                     LDVTALRRAQADLRQLNRDLEHRVVQEVASREAAQIRAAHAERMQALGQLAGGIAHDF
                     NNILQVVAGSAALIERRPDDVTAIIRIVRRIIDSANRGAAITRRMLIFARRGKLEAEA
                     VAPATLLEGLREICSYTLGPGIDIRVQLAPNLPRLVADKSQLETALVNMATNARDAMP
                     NGGVLTFSARLDVMASAMSDQAGLIPGAYIRLAVSDNGTGMDSTLLARVAEPFFTTKG
                     VGQGTGLGLSMVKGFAEQSGGSFSISSEVGAGTTVSLWLPQATNEAEAGSCETAEPGT
                     PLSAGGRLLLAEGEYPMRGTVATRLEA"
BASE COUNT          351 a          595 c          598 g          329 t
ORIGIN      
        1 gggacagccg aaccgcacgg gcattcggga aatcgacgat ctgggttggc aactttatcg
       61 cgcggccgag gaacgagccc gcgcgcaatc ggcgatggcc taccaaatca ccttgcttcg
      121 cgctgtgacc gaaagcaccg ccgatgcgat cttcatggtc gatccgcaag gcctcgtgac
      181 ttacgcgaat cccgaatccg cgcgcatgtt cggctggaac gaggacgaac ttatcggtca
      241 aacgctgcac catgtcgcgg acggccggcg caccagcggc agcgccaacg cggcctggga
      301 cagcaagctg acccgcacgg tcaccgccgg ccgggcatcc gttcgcgagg aagacatatt
      361 cttccgccgc gacggccaga tggtcatggt cgaatgtacc tactcgcccg tggttgtgga
      421 cgacgcgcca gccggcgccg tgctgaccgt ccgggacatc tcggcccgca aacgcatcga
      481 cgccgcactg cgcgaaaatg aggcgcggtt atgcgaactc gtgagcactc tcgacctggc
      541 gaaaattctg gttcgcgatc caaacggcac gattcggttc tggtcgttcg gctgtgaaag
      601 gctttacggc tggtccggtg ccgaggccat cggacgatcg actttctcac tgcttcaaac
      661 ggtcctcccg gtgccgcagg acgagatcaa cgcggtcttg ctgcgtgatg gggaatggtc
      721 gggggacctg atccagactt gccgcgacgg cagcctcatt accgtggcag tacgcagcgt
      781 gctgcaacgc ggcgcccaaa acgagcctcg cgcgatcatg gaaagcgtgc tggacgtgac
      841 ggcgctgcgc cgggcccaag ccgacctgcg gcagcttaac cgcgacctcg agcatcgggt
      901 cgtccaggaa gtcgccagcc gcgaagcggc acaaatccga gccgcgcatg cggaacggat
      961 gcaggcgctt ggccagttgg cgggcgggat cgcccacgac ttcaacaaca tccttcaagt
     1021 ggtcgcaggg tccgcggcat tgatcgagcg acggcctgac gacgtaacgg cgatcatccg
     1081 catcgttcgc agaatcatcg attccgccaa ccgcggcgcc gcgattacac ggcgcatgtt
     1141 gattttcgcc cggcgcggca agctggaggc cgaagctgtc gccccggcca cgctgctgga
     1201 aggtctgcgg gagatttgca gctatacgct tggcccgggc atcgacatcc gcgttcaact
     1261 cgcccctaat ctgccgcggc tggtcgccga caaaagccag ttggaaacgg cgctggtcaa
     1321 catggccacc aacgccaggg acgcgatgcc aaacggtggg gtgctgacgt tctccgcccg
     1381 tctggatgtg atggcttccg ccatgagcga tcaggccggt ctcattcccg gcgcctatat
     1441 ccggcttgcc gtgtccgaca acgggactgg catggacagc acgcttctgg cgcgcgtcgc
     1501 ggaacctttc tttaccacaa aaggtgtcgg ccagggcacc ggcctgggac tttccatggt
     1561 caaaggcttc gctgagcagt ccggaggcag cttcagtata tccagcgagg tcggagccgg
     1621 taccaccgtc agcctgtggc tcccgcaggc gacgaacgag gcggaggctg gctcttgtga
     1681 aaccgccgaa ccgggcactc cattgagcgc tggcggacgg ttattgctgg cggagggcga
     1741 gtacccgatg cgcgggactg tggcgacgcg gcttgaagcc tagaccattt gcggcctgat
     1801 tatgcaggcc gcaaatggtc tgcctcctta tttattgcgc aactcctcgc ttcggacgtt
     1861 ccgcccagcg tga
//
LOCUS       DASWQN010000736         3644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_863096, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000736 DASWQN010000000
VERSION     DASWQN010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863096"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1042)
                     /locus_tag="VGC82_12480"
     CDS             complement(98..1042)
                     /locus_tag="VGC82_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0424129.1"
                     /translation="MDQTTRVLDPMKFRDPLITAKGDKRATVTLRALETLWFNTGTLC
                     NLTCRNCYIDSSPRNDRLAYLTTAEVERYLDEVVRDHLPTKLIGFTGGEPLMNPDIIA
                     MLEAVLSRGFDALVLTNAMKPLRKLRTEMLALRERYGDRLTIRVSLDHYDPAIHEQER
                     GRRSWAPALDGLIWLARNGFKLDVAGRRFTGETEQSLREGYAALFAAAGIAVDAYDPV
                     RLMLFPEMEPESDVPEITTACWGILHKSPDDVMCAGARMVVKRKDASAPVVLACTLLA
                     YDPRFEMGTTLAEASGPVSLNHPFCASFCVLGGASCSR"
     gene            1130..2275
                     /locus_tag="VGC82_12485"
     CDS             1130..2275
                     /locus_tag="VGC82_12485"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0424130.1"
                     /translation="MAGALDHLLVLDLTSHLSGPYCAMLLADHGAEVIKIEPPKGDSA
                     RGMPPFINGESAPFMTWNRNKRSVVLDLKQSADRDALLDLIDRADILVENYRPGVLDR
                     LGLGWTALHQRNKRLILASISGFGQTGPYSSRGGFDLITQAMSGLMSTNGPADGPPHR
                     LPIAISDVTAGMFLAFGVLAAVEARHRTGEGQHVETSLLEAATSLAVYESAHYFATGT
                     RPERIGQAHRGSSPYQCFQTSDGYITVGASQQKFFEALCDLVGAPDLVADPRFASNAE
                     RVRHNDALVTLLAARISTQPSSHWLAALEKLGIPAGPVLHYDEVFTDPQILARDMVVE
                     TNHPVTGPFRTMGVTVKLSDTPGSIRRAAPRLGEHTNEVLNQRKAAE"
     gene            2275..3096
                     /locus_tag="VGC82_12490"
     CDS             2275..3096
                     /locus_tag="VGC82_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY0424131.1"
                     /translation="MSSQSEKAQRFRSLHETSETFVIANAWDAGSARILTSLGFKALA
                     SSSGACAGVLGRLDGMVTRDEALAHCKAIVDATDLPVSADLEKGFGDSPREAAETIRL
                     AGGIGLVGASIEDATGDTAHPLFDPSQATDRIAAAVQAARSMPFPFTLTARTENYLRG
                     NPDLQDTIRRLQAFEAAGADVLMAPGLPDLEAVRAVCAALSRPVNFMAGIKGKSFTVA
                     ALSEAGVRRVSLATSLYRAAITGFVAAAKEVAEHGTFGYLDGTIPTPAFNAFMKS"
     gene            3224..>3644
                     /gene="tsaA"
                     /locus_tag="VGC82_12495"
     CDS             3224..>3644
                     /gene="tsaA"
                     /locus_tag="VGC82_12495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEY0424132.1"
                     /translation="MTSFTIQPIGTLHTPWRTVSECPRNGRQPDPAPVCTVRVLPDFV
                     DGLLGLAGLSHLILLYWLDKATQPKLTFTPPFDPEPRGVFSTRAPFRPNPIGLSVVAF
                     DGFDGPDVLKVRFLDCVDGTALLDIKPYLATTDSEPSA"
BASE COUNT          672 a         1213 c         1074 g          685 t
ORIGIN      
        1 aacccgatct catgccgcac tggccagaac agttttttgg cgaccagttc tgccacttga
       61 accaggaagg tgccgaacgc ttcagcaacc aactcgctca gcgactgcag gaagcgccgc
      121 ccaggacgca gaacgaggcg cagaacgggt ggttgagcga caccggaccg gacgcctcgg
      181 ccagcgtcgt ccccatctca aaacgcggat cataggccag caacgtgcag gccagaacga
      241 ccggcgccga ggcatccttt cgcttcacca ccatgcgcgc accggcacac atgacgtcgt
      301 ccggcgattt gtgcagaatt ccccagcagg cggtggtgat ctccggcaca tcgctttctg
      361 gttccatttc gggaaacagc atcaaccgca ccggatcgta agcgtccacc gcgatcccgg
      421 ccgccgcgaa caacgcagca taaccctccc gcagcgactg ctcggtttcg ccggtaaagc
      481 ggcggccggc aacatccagt ttgaatccat ttcgggcgag ccagatcaac ccatccagcg
      541 ccggagccca gcttcgccgg ccgcgttcct gttcatggat agccgggtcg taatgatcga
      601 gcgacacccg gatggtcagc ctgtcgccgt atcgctcccg cagcgcgagc atctcggtcc
      661 gaagttttcg cagcggtttc atcgcgttcg tcagcaccag cgcgtcgaac ccgcgcgaca
      721 gcaccgcttc cagcatggcg atgatgtcgg ggttcatcaa tggctccccg ccggtgaagc
      781 caatcagttt tgtcggcaag tggtcccgaa cgacctcgtc cagatagcgt tcgacttccg
      841 ccgtggtaag gtaagccagt cggtcgttgc gcggcgacga atcgatgtag caattccggc
      901 aggtcaggtt gcacagcgtc ccggtgttga accacagtgt ttccagcgcc cgcagcgtga
      961 cggtggcccg tttatctccc ttcgcggtta tcaatggatc gcggaatttc atcggatcaa
     1021 gcacacgggt cgtctgatcc aaactcggtc tcctgcaggt tgtaagaccc gatgatggcg
     1081 catttccacc gctgtggcat catcgcccaa gcgataaagg aggaacatca tggccggtgc
     1141 tctcgatcac ctgctcgtcc tggacctgac cagccacctt tccggcccgt actgcgcgat
     1201 gctgctggcc gatcatggtg ccgaggtcat caagatcgag ccgccgaaag gtgactccgc
     1261 gcgcggtatg ccgccgttca tcaacggcga aagcgcaccg ttcatgacat ggaaccgcaa
     1321 caagcggtcc gtcgtgctcg accttaagca gtccgccgac cgggacgcac tgctggacct
     1381 gatcgaccga gccgacatcc ttgtcgagaa ttaccgccct ggcgtgctgg accggctggg
     1441 ccttggctgg accgcactgc atcagcgcaa caagcggctg atcctggctt ccatctcggg
     1501 gtttgggcag accggcccct acagttcgcg cggcggcttt gacctgatca cccaggcgat
     1561 gtccggactg atgagcacga acggccccgc ggacggcccg ccgcaccggt tgccgatcgc
     1621 catctcggac gtcaccgccg gcatgttcct ggcgttcggc gtccttgccg ccgtggaggc
     1681 acgccaccgc accggcgagg gccagcatgt cgagacgtcc ctgctggagg ccgccacgtc
     1741 gctggcagtc tatgaatccg cccactactt cgccaccggc acacggccgg agcggatcgg
     1801 tcaggcgcat cgcggaagct ctccctacca gtgctttcag acctcggacg gctacatcac
     1861 cgtcggtgcg tcgcagcaaa aattcttcga agcactgtgt gacctagtcg gtgcgcccga
     1921 tctggtcgcc gatccgcgct tcgccagcaa tgccgaacgc gtgcggcaca acgacgcgct
     1981 ggtgacgctg ctggcagcca ggatatccac gcagccatca tcccattggc tggccgcact
     2041 ggaaaagctg ggtattcccg ccggtccagt gctgcactac gacgaagtct ttaccgaccc
     2101 gcagatccta gcgcgggata tggtggttga gaccaaccat ccagtcaccg gcccgttccg
     2161 caccatgggc gtcacggtca agctttccga tacgcccggc tcgatcaggc gcgccgcgcc
     2221 acgcctgggc gagcacacga atgaggtctt gaaccaacga aaggcagcgg aataatgtcc
     2281 agccagtcgg aaaaagctca acgcttccgg agcttgcacg aaacgtcgga aaccttcgtt
     2341 atcgccaacg cctgggatgc cggttctgcc cgtatcctga cctcactcgg gttcaaggca
     2401 ttggcgagtt ccagcggagc ctgtgccggc gtgctcggcc ggctggatgg catggtcacg
     2461 cgcgatgaag ctctggcgca ttgcaaagcc atcgtcgatg ccaccgatct gccggtgtcg
     2521 gccgatctgg agaaaggttt cggcgactcc cccagagagg ctgccgaaac tatccggtta
     2581 gccggtggca ttggcctcgt cggcgcatcg attgaggatg ccaccggcga tacggcgcat
     2641 ccgctgttcg atccttcgca agccacagac cgcattgccg ccgccgtgca ggccgctcgg
     2701 tccatgccat ttccgttcac cctgacggcg cgaaccgaga attatctgcg cggcaacccc
     2761 gacttgcagg acacgatccg gcgcttgcag gcattcgagg cagccggcgc ggacgtgctg
     2821 atggcaccag gcctgcccga ccttgaggca gttcgtgccg tttgtgccgc gctgtcgcgc
     2881 ccggtcaatt ttatggccgg gatcaaaggc aagtcgttca ccgtggcggc attgtccgag
     2941 gccggggtca ggcgcgtcag cctggcgaca tcgttgtatc gcgccgccat caccggcttc
     3001 gtggcggctg cgaaagaggt cgccgaacat gggacatttg gttacctgga cgggacgatc
     3061 cctaccccgg cgttcaacgc gttcatgaag agctagtgca ttttcagcct gactgtacag
     3121 gccgcaaatg gtctatctcc ttgttttatc gcgtaattca cacgccccgg acgtttcgtc
     3181 cagcgtgatc atattctaga gcggcgcgct ggaaggagtc cgattgacca gtttcacgat
     3241 ccagcccatc ggcacgctgc acacgccatg gcgcacggta tccgaatgcc cgcggaacgg
     3301 ccgccagccc gacccggcgc cggtttgcac cgtgcgtgtg ttacccgact tcgtggacgg
     3361 gctgctcggc ctagccggct tgtcccacct tatcctgctg tactggctcg ataaggcgac
     3421 gcagccgaaa ctgactttta caccaccctt cgaccctgaa ccgcgcggcg tgttttccac
     3481 ccgcgctccg ttcaggccca acccgatcgg gctgtcggtc gtcgccttcg acgggttcga
     3541 tggcccggac gttttgaaag tccgcttcct ggactgcgtt gacggcaccg cgttgctcga
     3601 catcaagccc tatctggcga ccaccgacag cgagccgagt gccg
//
LOCUS       DASWQN010000737         4393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_71490, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000737 DASWQN010000000
VERSION     DASWQN010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71490"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VGC82_12500"
     CDS             complement(<1..758)
                     /locus_tag="VGC82_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010506807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0424133.1"
                     /translation="MQVVVVPNRLAPVVTTELNYLVGSNDSPDGFPGTAHALEHMMFR
                     GSEGLDRDQLAQLGALLGGVYNASTSETVTQYTYTVPADNLDVALRIEALRMRGLTLN
                     EADWQQERGAIEQEVSRDLSSPFYTFMAQAQAILFEHTPYQHDALGDRPSFDKTDAAL
                     LRQFYDRWYAPNNAILVIAGDVKPTEALAQAQAAFGDIPRRDVPLHAAVETVPVQPKT
                     LVLDTNFSVGLVAMVYPMPGLKHADFAAADVLSDV"
     gene            complement(1090..1995)
                     /locus_tag="VGC82_12505"
     CDS             complement(1090..1995)
                     /locus_tag="VGC82_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY0424134.1"
                     /translation="MSTELPANLEPWQWPEQTWRKIVGRARAGRSLKPDSWPGGARCA
                     VAISFDADHETIPLRDNDESPMRISQGQYGNRQAMPRILKLLERESVPASFFYPAVAA
                     LLHPDEVRAVAGDGHEIGIHSWIHEVNTALPREAERELTFRSRDTLAKLSGKEPVGIR
                     TASWDFSVNTLDIIREMKLLYDSSLMADDDPYELEDQGVPTGVVELPPEWIRDDAVYF
                     NMLRFSGLRPYTPPSAVEEIFTAEFDGAWDEGGMFLLTMHPHITGHRSRLPLLERLIR
                     HMKTKGSCWFATHAQVADWCNQRHG"
     gene            2062..3456
                     /locus_tag="VGC82_12510"
     CDS             2062..3456
                     /locus_tag="VGC82_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0424135.1"
                     /translation="MPDIDPALLSAEDMLAAYARQQLTPVDVLQAVTERVARLNPNLN
                     AFAAMNPAALQAAGESASRWRSGRPMGMLDGVPCTVKDLVDLAGFPTRRGSRTTSADP
                     VPDDAPMVMSLKAAGAVILGKTTTTEYGWKSPGDCPLHGITRNPWNPAYTTGGSSSGA
                     GAAGAAGFGPLHIGTDAGGSVRIPAAWCGLVGLKPTYGRIPQWPASAFASVSCAGPMT
                     RTVRDAALMFSAMARWDLRDPFCLPDDPRNWCGGIEDGVAGLTVGMLSNPGFDAPIDA
                     DGVAAVERAAQLLADAGAYVEQADAGLPDTSLVFGRVWGAALARLVAGMPTQVAGLLD
                     PGIREVAQTLGGMTAIEFMDAEATRAAAAHAMARLHQRYDLVLCPTVPAGPPLADAPT
                     VDPVRALWTAWAPWTFTFNLTRQPAISMPLGPRADGMPNSVQIAAAQFRDDLVLRAAR
                     AIEVALPIQVADLG"
     gene            complement(3680..>4393)
                     /locus_tag="VGC82_12515"
     CDS             complement(3680..>4393)
                     /locus_tag="VGC82_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0424136.1"
                     /translation="LEWFQIWSKLLEGGPEFDWDGRFFKLKRLQTDPVSVQRPRPPVM
                     SAGFSPRGRDFAARAADVLFLNVTELDQVPALLADVAQHMARYDRSIAVYTMAHVVCR
                     PTRRQAEDYFHYFAEEMADTEGQAYYRRNRGSTVPGATVLGATVSGAAASGAGAPIAR
                     PFENRFTRGTGQQFAGGYPGAYPFVGSPDDIAEEMGRMSATGLAGCTVAFVDYLAEIP
                     FFVQEILPRLERLGLRDKA"
BASE COUNT          787 a         1371 c         1458 g          777 t
ORIGIN      
        1 acgtccgaca ggacgtcggc agcggcgaag tccgcatgtt tcagtcccgg catcggataa
       61 accatcgcaa ccaggccgac cgagaaattg gtatcgagga cgagagtctt tggctgcacc
      121 ggaacagtct cgaccgcggc gtgcaggggc acgtcccggc gcgggatgtc gccgaacgct
      181 gcctgtgcct gcgccaatgc ctcggtcggc ttaacgtccc cggcgataac caggatcgcg
      241 ttgttcggag cgtaccatct gtcataaaac tggcgcagca gggcggcgtc ggttttgtcg
      301 aacgagggac ggtcgccgag agcatcatgc tggtacggcg tgtgctcgaa caagatggcc
      361 tgggcttgcg ccatgaaggt gtagaatggg ctggacaggt cgcgcgaaac ttcctgctcg
      421 atggcgccgc gttcctgctg ccaatcagcc tcgttcaacg tgaggccgcg catgcgcagg
      481 gcctcgatcc gcaacgccac gtccagatta tcggccggga ccgtgtaagt gtactgcgtg
      541 acggtttcgc tggtgctggc gttgtaaacg ccgcccagca gggcgccaag ctgagcgagt
      601 tggtcccggt caaggccctc gctgccgcgg aacatcatgt gttccagtgc gtgagcggta
      661 ccgggaaacc cgtccggcga gtcattggag ccaaccagat agttcaactc ggtcgtaacc
      721 accggagcca ggcggttcgg cacaaccacg acctgcattc cattggacag ggtcgcccgg
      781 agcacgctat ccgccggggt ggcggattca attgcgccgc atgggacggc taagacgatc
      841 agagcaaagc cagctgccca acggatcaca cactgtttca atatctactc ccgcaccgac
      901 agttatcggt aacaaatcta agcaatacag tgtgccgggg gcaacgaaat tgcggccgac
      961 acgaaattgc ggccgaccag ggaatgcgcg acgcggcctt ctccagccac gccggaccga
     1021 ggtgattgcc tcgcatggac gcgggtcgcc gctcggtatc aggaccggcg gggttttgga
     1081 cgcgctggct cagccgtggc gctggttgca ccagtcggca acctgggcat gcgttgcgaa
     1141 ccagcagctc cctttggtct tcatgtggcg gatcaggcgc tccagcagcg gcaaacgaga
     1201 gcggtgtccg gtgatgtgag gatgcatcgt cagcagaaac atcccgcctt cgtcccaggc
     1261 gccgtcgaat tcggcggtaa agatttcctc caccgccgat ggcggggtgt agggccgcaa
     1321 tcctgaaaac cgcagcatgt tgaagtacac agcatcgtcg cggatccatt ccggcggcaa
     1381 ctcgacaacg cctgtcggga cgccttggtc ttccagttca taaggatcgt cgtcggccat
     1441 caggctgctg tcgtacagca gtttcatctc acggatgata tccaacgtgt tgacggaaaa
     1501 atcccagctt gccgtgcgga tcccgacggg ctccttgccg ctgagcttcg ccaacgtatc
     1561 gcgcgaccga aaagtcagct cccgctcggc ttcccgtggc aaggcggtgt tgacctcgtg
     1621 gatccacgag tggatgccga tttcatgtcc gtcgccagca accgctcgga cttcgtctgg
     1681 gtgcagcagc gccgccaccg ccggatagaa gaatgacgcc ggcaccgact cgcgttccag
     1741 cagcttcagg attcggggca tcgcctggcg attgccgtat tgcccctggc tgatccgcat
     1801 ggggctttca tcgttgtcgc gcagcggaat tgtctcatgg tcggcgtcga agctgatcgc
     1861 cacggcgcag cgggcgcctc ctggccagga atccggtttc aggctgcggc cagcccgagc
     1921 gcggccaacg atcttgcgcc aggtctgttc gggccactgc caaggttcca ggtttgctgg
     1981 gagttcggta ctcattgagg gtttcatccc gttgctgccg gcgtcatgct gcacctagtt
     2041 cgacccattg gaaaagaggc catgccagac atcgaccctg ccctgctcag tgccgaggac
     2101 atgctggctg cctatgcgcg ccagcagttg acccccgtgg atgtgctgca ggcggtaacc
     2161 gagcgggtgg ctcggttgaa cccgaacctg aatgcattcg ctgcgatgaa tccggcggca
     2221 cttcaagcgg ccggggaaag tgcgtcccgt tggcggtcgg gccggccaat ggggatgctg
     2281 gatggcgtgc cctgcacggt gaaggatctg gtcgatctcg ccgggttccc gacgcggcga
     2341 ggcagccgga ccacaagtgc cgacccggtg cccgacgatg cgccgatggt aatgagcctg
     2401 aaagccgccg gggccgtcat actgggcaag accacgacaa ccgagtacgg ctggaagtcg
     2461 ccgggggact gcccgctgca cggcatcacg cgcaatccgt ggaaccctgc ctatacgacg
     2521 ggcgggtcgt cgtcgggcgc gggcgcggcg ggtgcggccg gatttggccc gctgcacatc
     2581 ggaacggatg ccggcggatc ggtgcgcatt ccggcggcgt ggtgcgggtt ggtcggcttg
     2641 aagcccacat acggcaggat tccgcaatgg cccgcgagcg cgttcgccag cgtctcctgt
     2701 gccgggccga tgacgcgaac cgtgcgcgac gcggcgctaa tgttctccgc catggcacgt
     2761 tgggacctcc gcgacccgtt ctgcctgccg gacgacccca ggaactggtg cggtgggatc
     2821 gaggacgggg tcgccggcct gaccgtgggt atgttgagta atcccggctt tgacgctccg
     2881 atcgatgccg acggtgtcgc ggcggtggag cgtgccgcgc agctgctggc tgatgccggc
     2941 gcttatgtgg agcaagcgga tgccgggctc cccgatacca gcctcgtgtt cggccgggta
     3001 tggggcgccg ccttagcccg gttggtcgcc ggaatgccga cccaggtggc gggcctgctg
     3061 gacccaggca tccgcgaggt cgcgcaaacc ctgggcggca tgaccgccat cgagttcatg
     3121 gacgccgagg caacccgagc cgccgccgcg cacgcgatgg ctcggttgca ccagcgctat
     3181 gatctggtgc tgtgtccgac cgtgcccgcc ggtccaccct tggccgatgc gccgacggtc
     3241 gatccggtgc gggcattgtg gaccgcctgg gcgccgtgga cgttcacgtt caatttgaca
     3301 cgccaaccgg cgatctcgat gccgttgggt ccgcgggcgg acggtatgcc aaactccgta
     3361 cagatcgccg cagcgcagtt ccgcgacgat ctcgtgctgc gtgcggcgcg ggcgatcgaa
     3421 gtggcactgc ccatccaggt ggcggatctc ggctaagccg cggagagtaa gcgattggtt
     3481 acattgccaa gtctgcgatc gcttacgtca aggcaccgcc aggggtgcga tcgagtggcg
     3541 gaccggttgc gcaaccttca ggcggatgca agtgcgtggc gcgcgccaca ggacgctggc
     3601 caacctgaag gatgaccgga tcatcgccgg cgttcctggc tcgagacgtc gatacagtgc
     3661 ggcgatcgcg ccgagcagac tacgccttgt cgcgcagtcc caagcgttcc aggcggggca
     3721 ggatctcctg cacgaagaat gggatttccg cgaggtaatc gacgaacgcg acagtgcagc
     3781 cggcgagccc ggtcgcactc atgcgcccca tttcctcggc gatgtcgtcg ggcgagccga
     3841 cgaagggata cgcgcccgga taaccgccag cgaattgctg cccggtgccg cgcgtgaagc
     3901 ggttttcgaa cggccgggcg atcggtgccc cagcaccgga ggccgcggcg ccagaaactg
     3961 tggcgccaag gactgtggcg ccagggaccg tggagccgcg attacggcga tagtacgcct
     4021 ggccttcagt gtcggccatt tcttcggcaa agtaatgaaa gtaatcctcg gcttgtcgtc
     4081 gggtcgggcg gcagaccacg tgcgccatgg tatagacggc aatgctgcga tcataacgag
     4141 ccatatgctg ggcaacatcg gccaacaacg ccggcacctg gtccaattcc gtcacgttca
     4201 aaaacagcac gtccgctgcc cgggccgcga aatcccggcc gcgcggggag aacccagccg
     4261 acatcaccgg tggacgcgga cgctggacag acacaggatc cgtttgcagc cgcttcaatt
     4321 tgaagaagcg gccgtcccag tcgaactccg gtccgccttc aagcagtttc gaccagatct
     4381 ggaaccactc cag
//
LOCUS       DASWQN010000738         4203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2446177, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000738 DASWQN010000000
VERSION     DASWQN010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2446177"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VGC82_12520"
     CDS             complement(<1..1017)
                     /locus_tag="VGC82_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY0424137.1"
                     /translation="MMFIFAADHAAHDPNRFTLPSDTRPYWEVPARADALLAALTAGG
                     LTAQSARDHGLDPIARVHDAGYLSFLQTAFARWRSVPGSGPILRAPAYAVRHKARRPD
                     AVAGQAGWYLSSVTAPIVEATWTAALGSAHAAIDAADIVLSGKTHAYALCRPPGHHAH
                     TDMAGGFCYLNNAAIAAQRMLDGGSGPVAILDFDVHHGNGTQQIFYERDDVHYVSVHG
                     DPAGLYPWYAGYADETGQGRGLGWNLNLPLPPGTRDADFAASVGKGLDAIGRKRPAAL
                     IVSVGFDAQEGDPAANLAVSADGFAMVGRQIAALGLPTVLIQEGGYLIERLGANLTAF
                     FTGFG"
     gene            1295..1771
                     /locus_tag="VGC82_12525"
     CDS             1295..1771
                     /locus_tag="VGC82_12525"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEY0424138.1"
                     /translation="MVTRSGENVTLRLADNVEVRLIVPLAIDAIKPGSFIGTAAMPQP
                     DGTLKAREVHVFPESMRGTGEGHRPWDLETGSTMTNGTVGDFAVANGRMLTLTYKGGE
                     QKVFVPESVPVITYEAGSKADLKTGAHVFVTGTHNGDESLTAVRIAVGKNGLVPPM"
     gene            complement(1924..2784)
                     /gene="cysE"
                     /locus_tag="VGC82_12530"
     CDS             complement(1924..2784)
                     /gene="cysE"
                     /locus_tag="VGC82_12530"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEY0424139.1"
                     /translation="MRPSPCHLMLVHPDDAAAITDALWARLRREAEAAYQREPKLASL
                     FFDSILNQPSFEAAVFHRVAARLKNDTISLPLIVQAFGKASEDDGCIVEAAKADIAAV
                     LDRDPACTRLIEPFLYFKGFHAIQAHRLNHWLWHNGERDFALYLQSRSSEVFQTDIHP
                     AAKFGHGIFLDHATGLVVGETAEVGDDVSMLHHVTLGGSGKETGDRHPKIRKGVVIGA
                     GAAILGNIEVGEHARVAAGSVVLKSVPAHATVAGVPAKVVRIVATDEPSRSMDQMLDN
                     LSYQSFNYTI"
     gene            complement(3076..4104)
                     /locus_tag="VGC82_12535"
     CDS             complement(3076..4104)
                     /locus_tag="VGC82_12535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY0424140.1"
                     /translation="MSRPPKLSRLKRDLHAIVHRSEYKPSPQEDRGRSATSPHQIPMP
                     GWKDILVRTWGEVSGNNIFLVAGGVTYAVLLALFPGLAALVSIYGLLLDPAQVERQVA
                     ALSNVLPASSAQMIGDELHKLVTAPSGSLSISAGVALLLALWSASRGMSGLITAFDIA
                     YEQKETRGFFKLNLVAIGLTVLMLIGGTVIIALVGVLPAALQLVGLESSTKWLLLVLE
                     WPILIVVVMSGLAVLYRYAPDRHPPRWRWVSPGAIVATVLWLLGSAGFSLYVTHFNSY
                     DKTYGSLGGVVVMLTWLYLSAFVALLGAVINAQSERQTRADSTVGGAKPLGTRKAQAA
                     DTVGEATS"
BASE COUNT          837 a         1285 c         1338 g          743 t
ORIGIN      
        1 cccgaacccg gtgaaaaacg cggtcagatt ggcgccgagg cgttcaatca ggtagccgcc
       61 ttcctggatc agcaccgtcg gcagacccag ggcggctatc tgacggccga ccatcgcgaa
      121 tccatccgcg ctgacagcga gattcgccgc ggggtcgcct tcctgggcat cgaagcccac
      181 cgacacgatc agcgctgccg gccgcttgcg gccgatggca tccagtcctt ttccgacaga
      241 ggccgcgaag tcggcatctc gtgtgccggg cggcagaggc agattgaggt tccatcccaa
      301 gcccctgccc tgcccggttt catccgcata accggcgtac caaggataca gaccggcggg
      361 gtcgccatgc accgagacgt aatgcacgtc atcgcgctca tagaagatct gctgcgtgcc
      421 gttgccgtga tggacgtcga agtcgaggat cgcaacaggt ccggaaccgc cgtcgagcat
      481 tcgttgcgcc gcgatcgccg cgttgttcag gtagcagaac ccgccggcca tgtcggtgtg
      541 cgcgtgatgc cccggcggcc ggcaaagcgc ataggcgtgg gtcttgcccg aaagaacgat
      601 atccgccgca tcaatggcgg cgtgcgccga accgagggcc gcggtccagg tggcctcgac
      661 aatcggcgcg gtgacgctgc tgagatacca tccggcctga ccggccacgg catcgggccg
      721 cctggcctta tggcgcacag catatgctgg cgctcgcaga attgggcccg aaccgggcac
      781 ggaacgccat cgggcaaagg cggtctgcag aaaggacaga tagccggcgt catgcacgcg
      841 ggcgatcggg tccagaccat gatcccgcgc ggattgggct gtcagaccgc cggcggtcaa
      901 tgccgcaaga agcgcgtcgg cgcgcgccgg aacttcccaa tacggacggg tgtcgctggg
      961 tagggtaaag cggtttggat cgtgagcggc gtggtcggcg gcaaatataa acatcatcga
     1021 tcctcaggtc ttccccttgc gggtagcagc ttacatcccg gatttttacc acgtgaatat
     1081 gttgattgct ttttctttcg aaatgtcgtc tggcgatctg atccggtaaa aaggtgaagt
     1141 ggcagtgtcg atatccagac gctcagttac catcgttccg gcgttcctgg gcttgggcgg
     1201 cttcgcatct gcccaaacgc cggccaagga ctcgtcgcct gtgccgatcc ggattcgggg
     1261 tgccatcgac aggatcgatg ggaaggaaat gcggttggtc acgcgcagcg gcgaaaatgt
     1321 cacgctcagg ctggccgaca atgtcgaggt aaggctgatc gtgcccctgg cgatcgacgc
     1381 aataaaaccc ggttcgttta ttggcacggc agcgatgccg caaccggacg gaacgctgaa
     1441 ggcgcgggaa gtacacgtct ttccggagtc gatgcgcggc accggtgaag gtcatcggcc
     1501 ttgggaccta gagaccggga gcacgatgac aaacggcact gtcggtgatt tcgcggtcgc
     1561 caacggccgc atgctgacac tgacgtacaa gggcggcgag cagaaggttt tcgtcccgga
     1621 aagcgtgcct gtgatcacct atgaagccgg cagcaaggcc gacctgaaaa ccggcgcgca
     1681 tgtctttgtt actggaaccc acaacggcga cgaatcactg accgcggtac gaatcgcggt
     1741 cggcaagaac ggtctggtgc ccccaatgta gccggacagg cgctgcggcc cgcacggtcc
     1801 tggcgacgcg caattgcgtg acggccgagc attaccaacc cgaccgcaca ggctggagat
     1861 gcattacctt cttgctttat acgatttaca cgcttcggac gttccgccca gcgtgatcat
     1921 gctctagatc gtatagttga aggactgata cgaaagatta tcgagcatct ggtccatcga
     1981 ccgggagggc tcatcggtcg cgacgatgcg caccaccttg gccggcacgc cggcgaccgt
     2041 ggcatgggcg ggcaccgact tcaggaccac cgaccccgcg gcaacccgag catgctcgcc
     2101 gacctcgatg ttgccgagaa tcgcggcacc tgctccgatc accacaccct tccggatctt
     2161 cgggtggcgg tcgccggttt ccttgccgct gccgcccagg gtgacgtgat gcagcatcga
     2221 gacgtcgtcg ccaacctccg ccgtctcgcc aaccacgagc ccggtggcat ggtcgaggaa
     2281 gatgccatgg ccgaacttcg ccgcaggatg gatgtcggtc tggaacacct ccgatgaacg
     2341 gctttgcaag tacagcgcga agtcgcgctc gccgttgtgc cacagccagt ggttcagccg
     2401 gtgcgcctgg atagcgtgga agcccttgaa atagaggaac ggttcgatca ggcgggtgca
     2461 ggcagggtcg cggtcgagca cggcggcgat gtccgccttt gctgcttcca cgatgcagcc
     2521 gtcatcctcg gaggccttgc cgaaggcttg cacgatcaac ggcagggaaa tggtgtcgtt
     2581 cttcaaccgg gcggcgacgc ggtggaaaac cgccgcttca aaactcggct ggttcaggat
     2641 cgagtcgaag aacagcgagg ccagcttcgg ctcgcgctga taagctgctt ccgcttctcg
     2701 acgtagccgt gcccacaagg catcggttat cgctgctgcg tcgtcggggt gaaccagcat
     2761 caagtggcac gggctcgggc gcatgtggtt gggtccgcag gttattggac gtccagaatc
     2821 ggacgattag ccatacatgt tcggatgatc gatggtttcg tcaactcccc tgcgcgtgcc
     2881 acggatcgat cgtgttgctt tcggatatcg acgagctgtc acgaacgtcg gccaagccat
     2941 tgttgttccc agtatttgca tcaaccggat aaccagtggg cgccggtcag tcctgttgct
     3001 gacgcatccg gtgcgcgccc ggatggagca ggccaggcaa tggacgcaac gggagcaagc
     3061 gaagtccgaa gccggctaag aggtcgcttc accgaccgtg tcggcggcct gagctttgcg
     3121 cgttcccagc ggcttggcgc cgccaacggt tgaatccgcc cgcgtctggc gttcggattg
     3181 cgcgttgatc acagcaccca acagcgcgac gaaggccgaa agataaagcc atgtcagcat
     3241 cacgacgaca ccgccaaggg agccgtaggt tttgtcgtaa ctattgaagt gggtgacata
     3301 gagcgagaaa ccggccgaac ccagaagcca caaaacagtc gcgacgatgg cgccagggga
     3361 gacccaccgc cagcgcggcg gatgacggtc gggggcataa cggtacaaca ccgccagccc
     3421 ggacatgacg acgacgatca ggatcggcca ttccagtacc agcagcagcc atttcgtgga
     3481 gctttcaagg ccaaccagtt gcagcgccgc gggcagcacg ccgaccagcg cgatgatcac
     3541 cgttccccca atcagcatca gcaccgtcaa gccgatcgcc accagattga gcttgaagaa
     3601 gccccgagtt tccttctgct cgtaagcgat gtcgaacgcg gtaatcaggc cactcatgcc
     3661 tcgcgaagca ctccagagtg ccagcagaag ggcgacgcca gcgctgatgc tgagcgaccc
     3721 gctgggagca gtgacgagct tgtgaagttc gtcgccgatc atctgcgcgc ttgacgccgg
     3781 cagaacgttc gacaaggcag cgacctgtcg ttcgacctga gccgggtcca gcaacaggcc
     3841 gtaaatggaa accagggcgg cgaggccagg gaacagcgcc agcaacacgg cgtaggtgac
     3901 cccgccagcc acgaggaaaa tgttgttccc ggatacctcg ccccaggtcc gtaccagaat
     3961 atccttccat cccggcatcg gaatctgatg gggtgaggtc gcggaccggc ccctgtcctc
     4021 ctggggcgac ggcttgtact cgctgcggtg cacgatcgcg tgcaggtctc gtttcagtcg
     4081 gctcagcttg ggcggccggc tcatggcgcg gtcccttcag ggaaaagctg aacaacgagc
     4141 cggccttttg gcggccggcg gtcacgctgc ggtgtcatcc gatccgggcg gctatgctgc
     4201 cga
//
LOCUS       DASWQN010000739         4434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_466985, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000739 DASWQN010000000
VERSION     DASWQN010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466985"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1811
                     /locus_tag="VGC82_12540"
     CDS             <1..1811
                     /locus_tag="VGC82_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0424141.1"
                     /translation="MGYISRTAVRHADDFPAMRGRPLAFLFSYIRRHPAGHLTVLIAV
                     LVAVTCSVSTQYGMKYLIDIVATGRDAAGDRVWGAFALLCGLVAADNLAWRVGLYTAH
                     RTFVAVTGDVRRDLFRHLAGHSPSYFAERLPGALASRISSTANAAFTLENTIAWNVIP
                     PTVALMLSICFIASVNLALAGTLLAISAVLGAIVFQMARKGSQRHRDFATKAAAVDGE
                     LVDIIGNFNVVRAFGATLREQRRVGERMETEMAARRRSLYYLEHIRLVHAVLTVFLTA
                     GVIAWAIILWQNGQAKVGDLVLITSLAFGILHGTRDLAVSLVDLTQHVVRLEEAISTL
                     LTPHDLENLPGAPALPVGPGEVAFERVRFNYPGRSAVLADFNLVIAPGERIGLVGFSG
                     AGKSTVLALLQRFFDVSGGRITIDGHDVRDVTQESLRNVMAVVPQDISLFHRSVLENI
                     RYARPDATEAEVLSAADMAHCRDFIEAMPDGFDTMVGDRGIKLSGGQRQRLAIARALL
                     KNAPILLLDEATSALDTESEQAIQHALDRLMTGRTVIAIAHRLSTLQRFDRIIVMDKG
                     RIVDDGSPYALASRPGLYRELLRKQRMEPAQAAEAA"
     gene            complement(1808..2476)
                     /locus_tag="VGC82_12545"
     CDS             complement(1808..2476)
                     /locus_tag="VGC82_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424142.1"
                     /translation="MLSQLGLPISDLDEPLVRQHANPAPRRAIHISGYDREGKDFYAT
                     PPWVTEALLQHVRFRAPVWEPCCGDGAMSSVLAHHGYDVVSTDLVDRGFGTPGVDFLL
                     SREVPGGCRSIVTNPPYGDTGSHAGQSRSPLAMLKFLRHAMMLTASVHGQLALLVRLQ
                     WAAGKRVAQTMSGGPFSALIVLTRRIQWFEMGEHTTTAQHHHAWVVFDHAHPAGQPPV
                     MLYA"
     gene            2720..4120
                     /locus_tag="VGC82_12550"
     CDS             2720..4120
                     /locus_tag="VGC82_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY0424143.1"
                     /translation="MSRLVVVSNRVAPIQEGEPTAGGLAAGVLDALKQKGGLWFGWSG
                     EVTSDAEPKPGAVRRVGPITLFTVDLTRKDYDEYYRGFANDALWPVLHYQIGLGHFEW
                     PAFEGYRRVNDRFAAALKPLVGADDLIWAHDYHLLCLAEALRRHGLTNRMGLFLHTPF
                     PSPGVFMTIPAHAELVQAMCAYDLLGFQTAIDRTAFIDYVMRHAGGIAIEADGVPDGA
                     VSVFGRLVRTGVYPIGVNVDEVRAQAETPVNRRQATRMRASLMERLILSVDRLDYSKG
                     LRQRFAAFERFLADFPAWHGLVTFMQIAPPSRSDIQAYQRIRRELEGEAGRVNGRFAE
                     VDWVPLRYLNRGFARNVLMPLYAEAQACLVTPLRDGMNLVAKEYVAAQDPEDPGVLIL
                     SQFAGAARELETALLVNPYDEAGMAQAMDRALSMPAEERRERHQAMLRVMRANSLERW
                     RDRFEADLRANFAAVR"
     gene            4216..>4434
                     /locus_tag="VGC82_12555"
     CDS             4216..>4434
                     /locus_tag="VGC82_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424144.1"
                     /translation="MGQTVDNEDLQEEHQTRADQSRSEQQTDRRDRAGNTSRPGDERH
                     DHEGRRDDDADSKPTRRWPLVVLGVVVLL"
BASE COUNT          768 a         1403 c         1462 g          801 t
ORIGIN      
        1 ctatggggta tatttctagg accgcggtcc ggcacgctga cgattttccg gccatgcgcg
       61 gtcggccgct ggcctttctc ttctcgtaca ttcgccgcca tcccgcggga catctgaccg
      121 tgctgatcgc ggtacttgta gcggtgacct gcagcgtttc cacccagtac ggcatgaagt
      181 acctcatcga catcgtcgcc accggtcgcg acgcggccgg cgatagggtc tggggcgcat
      241 ttgcgctgct ttgcggtctg gtggcggcgg acaatttggc ctggcgtgtc ggcctttaca
      301 ccgcgcaccg caccttcgtg gctgtcaccg gcgacgtccg ccgggatctg ttccgccatc
      361 tggccggcca ctctcccagc tacttcgccg aacgcctgcc gggcgcgctg gccagccgga
      421 tctcctcgac cgccaacgcc gccttcacac tggaaaatac catcgcgtgg aacgtgattc
      481 cgccgaccgt ggcgctaatg ctgtcgatct gcttcatcgc atcggtcaat ttggcgctag
      541 ccggaacgct gctggcgatc tccgcggtac tgggtgcgat cgtgttccaa atggcccgca
      601 agggctcgca gcggcaccgg gattttgcca ccaaagccgc cgcggtggac ggagagcttg
      661 ttgatattat tggaaatttc aacgtagtgc gagcgttcgg cgccacactg agggagcagc
      721 ggcgggtcgg cgagcggatg gagacggaaa tggcggcgcg gcgccgcagc ctgtactacc
      781 tggagcatat ccgcctggtt cacgcggtcc tgaccgtgtt cctgaccgcc ggcgtgatcg
      841 cctgggccat catcctctgg cagaacgggc aggccaaggt cggcgacctg gttctgatca
      901 catccctggc attcggcatc ctgcacggca ctcgcgacct tgcggtctca ctcgtggacc
      961 tgacccagca tgtggttcgg ctcgaggaag cgatctccac cctgctgacg ccgcatgacc
     1021 tggaaaacct gccgggcgcg ccagcgctgc cggtcggccc cggtgaggtg gcgttcgagc
     1081 gggtacgctt caactacccc ggtcgctccg ccgtgctggc cgacttcaac ctcgtgatcg
     1141 ccccggggga acggatcgga cttgtcggat tctccggcgc cggaaaatcg accgtgctgg
     1201 cattgctgca gcggttcttc gacgtcagcg gcgggcgaat caccatcgac ggccatgacg
     1261 ttcgcgatgt gacgcaggaa agcctgcgga atgtcatggc ggttgtgccg caggacatct
     1321 cgctgttcca tcgttcggtg ctggagaaca tccgttacgc gcggcccgat gcaaccgagg
     1381 ccgaggttct gtcggcggcg gacatggcgc attgccggga cttcatcgag gcgatgccgg
     1441 atggcttcga caccatggtc ggcgatcgtg gcatcaagct ttcgggcggg cagcggcagc
     1501 gcttggccat cgcacgggcg ctgctgaaga atgcgccgat cctgttgctg gatgaggcga
     1561 ccagcgcgct ggacaccgag tcggagcagg cgatccagca tgcactggac cggctgatga
     1621 cggggcgcac cgtgatcgcc atcgcccatc ggctttccac gctgcaacgc ttcgaccgga
     1681 tcatcgtcat ggacaaaggc cgcatcgtcg atgacggctc tccctacgcc ctcgcctccc
     1741 ggcccggcct ataccgggag ttgttgcgca aacagcgaat ggaaccggcg caagcggccg
     1801 aggcggctta ggcgtacagc atcaccggcg gctggccggc gggatgggcg tggtcgaaca
     1861 ccacccaggc gtggtgatgc tgcgctgtcg tggtatgctc gcccatctcg aaccattgga
     1921 tccggcgggt cagcacgatc agcgccgaga acggaccgcc ggacattgtc tgcgccacgc
     1981 gtttgccggc cgcccattgc aggcgcacca gcagcgcgag ctgaccatgc accgacgcgg
     2041 tcagcatcat cgcgtggcgc aggaacttca gcatcgccag gggtgagcgc gactggcccg
     2101 cgtgggaacc ggtgtcgcca tagggcgggt tcgtgacgat gctccggcag ccgccaggca
     2161 cttcgcggct gagcagaaaa tcgaccccag gcgtgccgaa gccgcggtcc accagatcgg
     2221 tggaaaccac atcgtagcca tggtgggcca agaccgagga catcgcgccg tcaccgcagc
     2281 aaggctccca taccggcgcg cggaaccgca catgctgcag caacgcctcc gtcacccagg
     2341 gcggggtggc atagaaatcc tttccttcgc gatcgtagcc ggaaatgtgg atggcccggc
     2401 gcggcgccgg gttggcgtgt tgccggacaa gcggttcatc aagatcactg atcggcaggc
     2461 caagttgaga taacatcgca gtcgctttcg gtcgaaaggt tcgtgattat cgggcctcgc
     2521 tgtgtcccga gcgaaacccg cgtccgccgc gactcggcct aaaaagcatg tcgtcgcggc
     2581 gcgggatttt accccgcggc gccaccaccg aacaaccacg agtagatgca cccgatgaca
     2641 gcctctctcg ccgccattag gctggccgcg gcgaatcgcg gagcatggtc cgcgcttttg
     2701 cggagtagcc gatcaggaca tgtcccgtct cgttgtcgtc tccaaccgcg tcgccccgat
     2761 ccaggagggt gagccaactg ccggtggttt ggccgccggc gtgctggacg cgctgaagca
     2821 gaagggcggc ttgtggttcg gctggagcgg cgaagtcacc agcgacgccg aaccaaagcc
     2881 cggagccgta cgccgcgtcg gcccgatcac gctgttcacc gtggacctca cacgcaagga
     2941 ctacgacgaa tattatcgcg gcttcgccaa cgatgcgctt tggccggtgc tgcattacca
     3001 aatcgggctg gggcactttg aatggccggc gttcgaaggc taccggcgcg taaacgaccg
     3061 atttgccgcc gcgctgaagc cgctggtagg cgccgatgac ctgatctggg cgcatgatta
     3121 ccatctgctt tgcttggcgg aggcgctgcg ccggcatggc ctgaccaatc ggatgggctt
     3181 gttcctgcac acccccttcc cttcccccgg cgtcttcatg acgatcccag cgcatgcgga
     3241 gctggttcaa gcgatgtgcg cctacgattt gttggggttc cagacggcga tcgaccgcac
     3301 cgcgttcatc gactacgtaa tgaggcatgc cggcgggatc gccattgaag ccgacggcgt
     3361 gcccgacggc gccgtctcag tgttcggacg gctcgtgcgg actggcgtct atccgatcgg
     3421 cgtgaatgtg gatgaggtgc gggcacaggc ggaaacaccg gtcaatcggc ggcaggcgac
     3481 gcggatgcgg gcgagcctga tggagcggct gattctcagc gtggaccggc tggactactc
     3541 gaaggggttg cgccagcgct tcgcggcgtt cgaacgcttt ctggcggatt ttccggcgtg
     3601 gcacggtctc gtgaccttca tgcagatcgc accgccctcg cgctccgaca tccaggcata
     3661 ccagcgcatc cggcgcgaac tggagggcga ggccgggcgc gtcaacggac gcttcgccga
     3721 ggttgattgg gtgccgttgc ggtacctgaa ccgcggcttc gcacgcaacg tcctgatgcc
     3781 attatatgcc gaagcccagg cttgcctggt gacgccgctg cgcgatggga tgaaccttgt
     3841 tgccaaagag tacgtcgcgg cacaggatcc cgaggatccc ggcgtgctga tcctgtcgca
     3901 atttgccggt gcggcgcggg aactggaaac ggcgctgctg gtcaatccgt acgatgaggc
     3961 agggatggcc caggcaatgg accgggcgct ttcgatgccg gcggaagaac gccgggagcg
     4021 tcatcaggca atgctcaggg tcatgcgggc caacagcctg gaacggtggc gtgaccgctt
     4081 cgaagcagat ctgcgcgcga attttgcggc cgtcagatag cccttcgcca tctccggaca
     4141 gcctcgcttt gatgtgaaac caacatccgc cgaagtctgc gttgtcgtcc tgcggcgata
     4201 ttcgggagtt gggctttggg gcaaaccgtc gataacgagg atctgcagga ggaacatcaa
     4261 accagggccg accagagccg aagcgaacaa caaaccgatc gccgcgatcg tgcgggcaat
     4321 accagccgcc ccggcgacga gagacacgat cacgaagggc ggcgggatga cgacgccgac
     4381 agcaagccga cgcgccggtg gccgcttgtc gtccttggcg tcgtggtgtt gctg
//
LOCUS       DASWQN010000740         4655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_863470, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000740 DASWQN010000000
VERSION     DASWQN010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863470"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VGC82_12560"
     CDS             <1..1333
                     /locus_tag="VGC82_12560"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEY0424145.1"
                     /translation="ASGQLGYGIPAASFNSGIDRRPDLIGCDMGSIDIGPTYLGKGEM
                     ATAPEATRRDLRRVLQAARALDVPLVIGSAGSAGASPHLDATLAMVRNIARADGHRFR
                     MAVMRADIPRPTLRGAVRAGKVAGFDGMPALTEEDVTEAAHIVGQMGMGPFRKALESD
                     VDVIIAGRACDTAIFASLPVVLGFPTGLAMHMAKIIECGSLCCVPGGRDPILAELDHD
                     GFVLESMNPDRRATPTSVAAHSLYEQSDPFTVQEPDGTLDLSLADYQAVDDRRTRVSG
                     ARWRDTTQPTIKLEGARKIGERAVLLCGSADPRFIEQCKALLPKVADLVQSLVCEEQP
                     IDYTLTFRVYGIDGVRMVVPENEALPGEVFIMGECIAPTRERASEVVRTCKQFLLHFG
                     YPGRLSTAGNIAFPFTPPEVSLGPAYRFSVYHLLHVDDADALFPVEVETVG"
     gene            complement(1407..2345)
                     /locus_tag="VGC82_12565"
     CDS             complement(1407..2345)
                     /locus_tag="VGC82_12565"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0424146.1"
                     /translation="MAIFVIGGTGFIGIRVIPLLVARGESVVCMDINPNAPSLAGLGD
                     KVSVVRGDVTQFDDVISVMEASKADRVINLSYNLGQDLPPHRATKLNIVGMDNCFEAA
                     RILGVKHTVFASSLAVSGQQSHFGERLVTEDDYKHAVVQYGSHKAFNEFQAKDYIDKH
                     GMTITAIRPANVTGPDKVRGSVDHVNIITRPARGEPVSFPFADAMRCPIHVDDIAEVF
                     VRVLMADKPAHAIYNSGGQAISLAEIAAIVREYLPDAQISFEKQTGGKDGSGNWLIDN
                     TRLVQEFGVQYRPYRWRVLQIINDCRTEIGQPPIRA"
     gene            complement(2468..4078)
                     /locus_tag="VGC82_12570"
     CDS             complement(2468..4078)
                     /locus_tag="VGC82_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424147.1"
                     /translation="MTLRIPLPATAPFAAVALFTAIAAFAAWAAIPAEAATFRWANDG
                     DVNAMDPATRQETVQLSFLSNIYEPLVRRNRSLGLEPALATSWEQTSPTVWRFHLRPG
                     VKWQDGSPFTADDVVFTLKRIQAPNSSMRAPMAPVKEARKIDDLTVDFETTVPDPIFL
                     QEQTNLLIMSKAWCEAHNSSEPVTIGKDDNYAIHYAMGTGPFKQLSREPDRKTVLEKN
                     PLWWDKPEHNLDRVEFNVISSAPTRVAALLSGEVDMIYSVPPQDIARIKQTDGLKLLQ
                     TPELRTVYLGFNQSRDELKSSDVKGKNPFRDLKVREAVALAIDEPAIANRVMLGLGHP
                     TWEMWGPGVNGYDAALDMRPKPDPAKAKQLLADAGYPNGFRVGLDCPNDRYVMDEQIC
                     TAIVSMLARIGIKTDLHAQTKSKFFNQILSPNYDTDFWMLGWTPATYDGHNVLYTLLA
                     TRNGKRGEVNVGGYSNPVLDDLIESIGVETDVVKRTAMIHQSIEILQKDLPALPLHQQ
                     VIVWAARNNVEMAQPADNFLPYRLVQMK"
     gene            4162..>4655
                     /locus_tag="VGC82_12575"
     CDS             4162..>4655
                     /locus_tag="VGC82_12575"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEY0424148.1"
                     /translation="MQIDSRTDIVGLVDIQPTFMPGGELAVAQGDDIIPVVNRLLGLF
                     DHAFATQDWHPADHVSFASVHPGKRPYDTMDMPYGAQVLWPDHAVAGTPNAAIHTGID
                     QTRIEAIIRKGFRRTLDSYSALFENDRRTPTGLEAWLRQRGFSQLFLCGLATDFCVAW
                     SAEDA"
BASE COUNT          847 a         1522 c         1386 g          900 t
ORIGIN      
        1 agcttccggc caactcggct acggcatccc cgcggcgtcg ttcaattccg gcatcgaccg
       61 ccgcccggac ctgatcgggt gcgacatggg gtcgatcgac atcggcccga cctatctggg
      121 caagggcgag atggcgaccg cccctgaagc cacgcgccgt gaccttcgcc gtgtcctgca
      181 ggctgcccgt gccctggacg tgcccctggt catcggctcc gcgggatcgg ccggagcgag
      241 cccgcatctt gatgccaccc tggcgatggt ccgcaatatc gcccgcgccg acggccaccg
      301 cttccgcatg gcggtgatgc gcgccgacat ccctcgtccg acacttcgag gcgctgttcg
      361 cgccggcaaa gtcgccggct tcgacggcat gcccgcgctg accgaggaag acgttaccga
      421 ggccgcacac atcgtcggcc agatggggat ggggccattc cggaaggcgc tcgaatccga
      481 tgtggacgtc atcattgccg gccgagcctg cgacaccgcg atcttcgcct cgctgccggt
      541 tgtgctggga ttccccaccg gcctcgcgat gcacatggca aagatcatcg agtgcggttc
      601 actctgctgc gtcccgggcg gacgcgaccc gatcctggcc gaactcgacc atgacggctt
      661 cgtcctcgaa agcatgaatc cagatcgccg cgccaccccc acatccgttg ccgcgcatag
      721 cctgtatgag caatccgacc cattcaccgt acaggaaccg gacggcacac tcgacctgag
      781 cctggccgac taccaggcgg tggacgatcg ccgaacccgc gtcagcggcg ccagatggcg
      841 cgacaccacc cagccgacca tcaaactcga aggcgcgcgc aagatcggcg agcgcgccgt
      901 cctgctgtgc ggttcggccg atcctcgctt catcgagcaa tgcaaggcgc tgctgccgaa
      961 agtcgccgac ctggtgcaaa gcctggtctg tgaggaacag ccgatcgact acacgctgac
     1021 gttccgcgtt tacggcatcg acggcgtgcg catggtggtc ccggagaacg aagcccttcc
     1081 cggggaggtc ttcattatgg gcgaatgcat cgccccgacc cgcgagcggg cgtcagaggt
     1141 ggtgcgtacc tgcaagcaat tcttgctgca tttcggctat cccggccgcc tgtcaaccgc
     1201 cggcaacatc gcctttccat ttacgccgcc ggaagtttcg ctcggtcccg cctatcgctt
     1261 cagcgtttat cacctgttgc atgtggacga tgccgacgca ttgttcccgg tggaggtcga
     1321 aacggtggga tagggcgcga acctgggacg tcggcgcttt ggttcaccaa ccagataggg
     1381 aacgcgctaa cagcgctcgc ggccgatcaa gcccggattg gcggctgccc aatctcggtc
     1441 cggcagtcgt tgatgatctg cagcacgcgc caccggtaag gccggtattg cacaccgaac
     1501 tcctgcacca aacgtgtgtt gtcaatcagc cagttgcctg acccgtcctt accgccggtc
     1561 tgcttctcga acgagatctg ggcgtccggc aaatactccc gcacgattgc ggcgatctcc
     1621 gccagcgaaa tggcctggcc gcccgagttg tagatcgcgt gcgccggctt gtcggccatc
     1681 agcacgcgaa cgaaaacctc cgcgatatca tccacatgaa tcggacaccg catcgcgtcg
     1741 gcaaacggga aggaaaccgg ctcgccccgc gccgggcggg tgatgatgtt cacatgatcc
     1801 accgaaccac ggaccttgtc cggcccagtg acattcgccg ggcgaatggc ggtgatcgtc
     1861 atgccgtgct tgtcgatgta gtccttggcc tggaattcgt tgaacgcctt gtgcgacccg
     1921 tactggacca ccgcgtgctt gtaatcgtcc tcggtcacca agcgttcgcc gaaatggctc
     1981 tgctggccgc tgaccgcgag ggagctggcg aagacggtgt gctttacgcc aagaatgcgc
     2041 gccgcctcga agcagttgtc cataccgacg atattcagtt tggtcgcgcg gtgcggcggc
     2101 aaatcctgcc ccaggttgta cgacaggttg ataacgcgat cggccttcga cgcttccatg
     2161 accgagatga cgtcgtcgaa ctgggtgacg tcgccgcgca ccaccgatac cttatccccg
     2221 agaccggcga gcgagggcgc atttggattg atgtccatgc acacgaccga ctcaccgcgc
     2281 gcgaccagaa gcggaatgac gcgaatgccg atgaacccgg tcccgccgat aacgaagatg
     2341 gccatgtcgt tttctccctt cccacgggtc tcgcccgctt tacggcagga tcggacgaag
     2401 cgttcggttc ggcaagagcc gatgccggcg gaccgacacc accgtcacgc gagacaaggc
     2461 ggatcgatca cttcatctgg accaatcggt atggcaggaa gttatccgcc ggttgcgcca
     2521 tctcgacgtt gtttcttgcc gcccagacga tcacctgctg gtgcagcgga agggccggca
     2581 gatccttctg caggatctcg atcgactgat ggatcatcgc cgtccgttta acgacatcgg
     2641 tttcgacgcc gatactctcg atcaaatcgt caagcaccgg gttggagtag ccaccgacgt
     2701 tgacttcccc ccgcttgccg ttgcgcgtgg ccagcagcgt gtacagcacg ttgtgcccat
     2761 cataggtcgc cggcgtccaa cccagcatcc agaagtcggt gtcgtaattc ggcgacagga
     2821 tctggttgaa gaacttcgat tttgtctggg cgtgcagatc ggtcttgata ccgatccggg
     2881 ctagcatcga cacgatggcg gtgcagatct gctcgtccat gacgtaacgg tcgttggggc
     2941 aatccagtcc gacccggaag ccgttggggt agcccgcatc ggccagcaat tgcttcgcct
     3001 tcgccgggtc gggcttcggc cgcatatcca gcgcggcatc gtatccattc acccccggcc
     3061 cccacatctc ccacgttgga tgcccaagcc ccagcataac ccggttggcg atcgccggct
     3121 catcgatggc cagcgcgacc gcttcccgca ctttcaggtc gcggaacgga tttttgccct
     3181 tcacatccga gcttttgagc tcatcgcggc tttggttgaa gcccagatag accgtccgca
     3241 actccggcgt ttgcaacagt ttcagaccgt cggtctgctt gatccgggcg atgtcttgcg
     3301 gcggcacact gtagatcatg tcaacctcgc ccgacagcag agccgccacc cgcgtcggtg
     3361 ccgacgagat cacgttgaac tctacccggt ccaggttgtg ctcgggcttg tcccaccaca
     3421 gcggattctt ctcgagcaca gtcttgcgat ccggctcccg cgagagctgc ttgaacggcc
     3481 cggtccccat cgcgtaatga atggcgtagt tgtcgtcctt gccaattgtt acgggttcgc
     3541 tggaattgtg cgcctcgcac caggccttcg acatgatcag caggttggtc tgttcctgca
     3601 ggaagatcgg gtctggcacg gttgtctcga aatccaccgt cagatcgtcg atcttccgag
     3661 cctccttgac cggtgccatc ggcgcccgca ttgaagagtt cggcgcctgg atccgcttca
     3721 acgtgaacac cacgtcgtcg gcggtaaatg gtgaaccgtc ctgccacttc actccgggcc
     3781 gcaggtggaa acgccacacg gtcggggatg tctgctccca cgatgttgcc agcgccggct
     3841 caagaccgag cgagcgattg cgccggacca ggggctcata aatgttcgag agaaaactaa
     3901 gctgcaccgt ttcctgcctt gtggcaggat ccatcgcgtt gacgtcgccg tcattggccc
     3961 agcggaaggt cgccgcctcg gccggtatcg ccgcccaggc ggcaaacgct gcgattgcgg
     4021 taaataaagc aaccgcggcg aacggtgccg tcgcgggtaa tggaatgcgc agcgtcatgg
     4081 tttgcccccg gtgtccttgg tagttgagcc caacggcgga aagctaaccg taccgggcgg
     4141 ttgtgaacag ggagagtgca gatgcaaatc gattcccgca ccgatatcgt gggtttggtc
     4201 gatattcagc cgacctttat gcctggcggc gagttggcgg tcgcacaggg cgatgacatc
     4261 attccggttg taaaccggct gcttgggtta ttcgatcacg cattcgcaac gcaggattgg
     4321 cacccagcgg accacgtctc gtttgccagc gtccatcccg gcaagcgccc ctacgacacc
     4381 atggacatgc cgtatggcgc ccaggtgctg tggcccgacc atgctgtcgc cggcacgccg
     4441 aatgccgcca tacataccgg aatcgaccag acccgcatcg aagcgatcat ccgcaaaggc
     4501 tttcgccgaa cgctcgacag ctattccgca ttgttcgaaa acgaccgccg aaccccgacc
     4561 gggctggagg cgtggctccg ccagcgaggc ttctcccagc tgttcctgtg cggcctggct
     4621 accgatttct gcgttgcatg gtccgccgag gacgc
//
LOCUS       DASWQN010000741         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1522130, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000741 DASWQN010000000
VERSION     DASWQN010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522130"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VGC82_12580"
     CDS             complement(<1..727)
                     /locus_tag="VGC82_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY0424149.1"
                     /translation="MIEPNLIWPIVAAPFVGSFLGVLIIRLPAGTPLAMVRSACQHCG
                     TALGPLDLVPLISFVVLRGRCRYCGGNIGLFHPAVELAALGIAILAVLGDPDPGWAWV
                     ACALGWTLLTLAWIDWTDFLLPDVLTLPLLLGGLVLTRIWDPDSLTDHCLAAALAYLS
                     FRGIAVVYRLLRGRDGLGGGDAKLFAAAGAWCGLGALPLVVLGSAVIGLLAALGLALA
                     GRNMTSTTRIPFGPCIALAFWLVW"
     gene            816..>2223
                     /locus_tag="VGC82_12585"
     CDS             816..>2223
                     /locus_tag="VGC82_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY0424150.1"
                     /translation="MGTTVTDHQLDALSDILLRQGHCDQRTLERARRVAIDSDQRLDA
                     VLIQLGLVSERGLADAYADLLSIPVTSVDRYPPLEPVLPDRLGATFLRHARAIPLAAD
                     VQTVTLAAADPLDPFTQAAVAVATGRQVRLQVAVPIELETALNRLYPEEGSATPDAGA
                     DAPLEEDAERLKDLASEAPVIRLVNLLITRAVETQASDIHIEPFEDRLRVRYRYDGVL
                     HEAEPPPRHLAAAITSRIKIMARLDIAERRMPQDGRIKMAVRGQDVDFRVSTIPSLHG
                     ETVVLRILDRSAVVFDYARLGLAPGVIRKLGSALDLPNGIVLVTGPTGSGKTTTLYTG
                     LLALNAITRKIVTVEDPIEYQLGGINQIQVRAQIGLTFASLLRSILRQDPDVIMVGEI
                     RDGETAQIAVQAALTGHLVLSTLHTNSAAAAVTRLRDMGVEDYLLTAVLRGIMAQRLV
                     RRLCLDCRRREPAPPELVQ"
BASE COUNT          429 a          763 c          670 g          361 t
ORIGIN      
        1 tccacaccag ccagaaagcc agggcgatac aagggccgaa cggaatccgg gtcgtggatg
       61 tcatgtttcg gcctgccagc gcgagtccca aggccgcgag cagcccgatc accgcgctgc
      121 ccagcacgac cagcggcaac gcgcccaggc cgcaccacgc tccggccgcg gcaaacagct
      181 tggcatcacc gccacccaga ccgtcgcgtc cgcgcagcag ccgatatacc acggcgatcc
      241 ctcggaacga caaatacgcc agggccgccg ccaggcagtg atccgtcagc gagtccggat
      301 cccagatgcg tgtcagcacc aacccgccca gcaacagcgg caacgtcagc acgtcaggca
      361 gcaggaagtc cgtccagtcg atccacgcca gcgtcagcaa agtccaccct agcgcgcaag
      421 caacccaggc ccacccagga tccggatcgc ccagcacagc caggatcgcg atccctaatg
      481 cggccaattc aacggctgga tgaaatagcc cgatgtttcc accgcaatac cggcaccgtc
      541 cgcgtaggac gacaaaactg atcaggggca ccaggtcgag agggcccaac gcggtcccac
      601 aatgctggca ggccgaccga accattgcga gcggggtgcc agccggcagc cggatgatca
      661 gcaccccaag aaagctgccg acaaacggcg cggccactat gggccaaatc aggttcggct
      721 cgatcacggg aaactttcgt cgaaagtcag tcgctgacct gtcacttgcc cctcatctca
      781 cggcggctta tgcgtacttc aggaacgcgg ccgcgatggg aaccaccgtc accgatcacc
      841 aacttgacgc acttagcgac atattgctgc gtcagggtca ctgcgaccag aggaccctgg
      901 agcgggcgcg tcgcgtggca atcgactccg atcagcgact ggacgcggta ctgattcagt
      961 tgggcctggt ttcggaacgt ggcctagccg acgcatacgc cgatcttctg tccattcccg
     1021 ttaccagcgt cgaccgctat ccgccgttgg aacctgtgct gccggatcgc ctcggtgcca
     1081 cgttcctgcg acacgcgcga gcgattcccc tggcggcaga tgtgcagacc gtcacactgg
     1141 ccgccgcgga tccgctcgat ccgttcaccc aggcggcggt tgccgtcgcg accggacgcc
     1201 aggtgcgact gcaggtcgcg gtgccgatcg aactggaaac ggcgctgaac cgcctttatc
     1261 cggaagaagg ttccgcgacg cccgacgccg gcgccgacgc gcccctggag gaggacgcgg
     1321 aacgtctgaa agacctcgcc agtgaggcgc ccgtcatccg cctggtcaac ctgttgatta
     1381 cccgagcggt ggagacccag gcgtctgaca ttcatatcga accgttcgag gaccgcctgc
     1441 gtgtccgtta ccgctacgac ggtgtcctgc atgaagcgga accgccgccg cgtcaccttg
     1501 ccgccgcgat cacctcacgc atcaaaatca tggcgcgcct cgatatcgcc gaacggcgga
     1561 tgccgcagga tgggcgcatc aagatggcgg tgcgtggtca ggacgttgat tttcgtgtgt
     1621 ccacgatccc gtcgctgcat ggcgaaacgg tggtgctgcg catcctcgat cgctccgccg
     1681 tggtgtttga ttacgcgcgc ctgggactgg cgcctggggt tatccgtaag cttggatcgg
     1741 cgctggacct gccgaatggg atcgtgcttg tcaccgggcc gaccggcagc ggcaagacca
     1801 cgacgttata cactgggttg ctggcgctga atgccatcac ccgcaagatc gtcaccgttg
     1861 aggacccgat cgagtatcag ctcggcggca tcaatcagat ccaggttcgc gcccagatcg
     1921 gcctgacctt cgccagcctg ttgcggtcga tccttcgcca ggacccggac gtcatcatgg
     1981 tgggcgaaat tcgcgacggc gaaaccgccc agatcgctgt ccaggcggcg ctgaccggac
     2041 atctcgttct ttcgacgctg cacaccaact ccgctgccgc cgccgtcacc cgcctgcgcg
     2101 acatgggcgt tgaggattat ctgctgaccg cggtcctccg cggcatcatg gcgcaacggc
     2161 tggtccggcg actttgtctc gactgtcggc gtcgcgaacc ggcaccgccg gaactggtcc
     2221 agc
//
LOCUS       DASWQN010000742         7792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1919012, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000742 DASWQN010000000
VERSION     DASWQN010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1919012"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..508
                     /locus_tag="VGC82_12590"
     CDS             200..508
                     /locus_tag="VGC82_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424151.1"
                     /translation="MTTMVWPLRRTCLAVAALTIAPMPARAEFFDGARRTFQTDIPHF
                     FQDDIPCAFGGQPTSHTKTSCKSSDHSAKRAPARTGDRDAVSPAKADDAHNVPAKSNR
                     "
     gene            706..1830
                     /locus_tag="VGC82_12595"
     CDS             706..1830
                     /locus_tag="VGC82_12595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0424152.1"
                     /translation="MNFEFSDELLQLREQARRFLTDRCSPSVPRRILDTDEPYAADLW
                     QAVAEMGWTGAAIPEEYGGVGLGRLAVCVLAEELGRAVAPIPFSSSVYLATEAIMLFG
                     SEQQKQAWLPKLATGEIIGTFALAERPGSSDPHKLTASVNAGRLSGTKLAVPDGDIAD
                     LAIVAVNGGNGAWLHLVDLKGAGVTRGTVVTFDPTRSHARIEFSDARAEPLPDSLGAE
                     TVRTLLDYAAVPMAFEQVGGAQAALDMANAYAKERFAFGRPIGSFQGIKHKLADMYIQ
                     VELARSNAYYGAWAVHTGAAELPIAASVARIAASEAGWFVTKENIQTHGGMGFTWQMD
                     CHLYYRRAKLLSLALGGAREWKHRLVRELRARNTTDLAAA"
     gene            1841..3076
                     /locus_tag="VGC82_12600"
     CDS             1841..3076
                     /locus_tag="VGC82_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0424153.1"
                     /translation="MDFEDTPDEAAFRAEARAFLDANAERRKPGEVEGYRRGQDAPGA
                     MQRAKDFQRRKYDAGFVGIHWPTEWGGRGGTPIQHVIYNQEEAKYDVLTGLFGIGINL
                     AQPTICTWGTPEQRDRFVKPAMRADEVWCQLFSEPDGGSDLAALRTRAERDGDEWVVN
                     GQKIWNSGAHYSDFGILVARSNPDVPKHEGLTFFILDMKTPGIEIRPIRQVSGSSHFN
                     EVFFTNVRIPDSRRVGPVGAGWKVALTTLMNERHGMRDAPGPDVAELFRLAQSIELED
                     GPAIDDSAVQEKLAEFYTRSQGLKYTKFRGITALSKGQTPGPESSIIKVVAANKLQDI
                     LSYGLDMIGMAGPTMGDAMPVAGIFEEALLYSPALRLAGGTDEIMKNIIAERVLGLPQ
                     DPRADKGLPFRDIPTGVRK"
     gene            3073..5205
                     /locus_tag="VGC82_12605"
     CDS             3073..5205
                     /locus_tag="VGC82_12605"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY0424154.1"
                     /translation="MTSVNATTHKLHSFFWPESIAVLGASPDLHRIRGRLLHQLRENG
                     FPGRILPINPSYQEIEGLACYPSIGAAGGGGIDLALIAIPAAGVAAAVEECARAGVKN
                     TLIISSGFAEEGGAAGDMQAELLAVTQRTGIRACGPNCEGYYNALGRVATTFSPTVET
                     REDDGRVLVSDKRIGVIAQSGGIGFALFNRGKAAGLGFSYVISTGNEADLGMADFLDY
                     MIEDPHTHAVMLFCEAVRNGPGFVAALEKARRLGKPVIAIKVGRSDAGTRATASHTAS
                     LSGSYAAYHAVFGRYGVIEAEDADEAVAIAGLVLTCPLPKGRRAGIITVSGGGGAWMA
                     DTLSAHGLIVPNLSAGSQSALRPLMPSYGAAGNPVDVTAQGSNTGPAMMTVMEHLAGS
                     DEIDIVVLVTSLASEVRASLDAARIRAVAASCGKPMTVWSYTLPSAFGREAAAGCGLF
                     VDSNLRNIGVAMGKLAGYAEALQRQLPEPFAPVSGRLYPGLPAVVPEYLAKQVLAAWL
                     PDTREKLVTSAEAAADAAQHLGFPAVLKVQSPDLPHKTEAGGVRLNLVDRDAVINAYT
                     AMVADVGRHAPGASIDGVLVQRMAPKGHELVIGIVDDPTFGPIMMLGFGGTTVELFGD
                     VVHAPAPVDEAEATRMILSLKSARLLTGFRGAKPIDLAPVARLVASLSRAALTLRDQV
                     REFELNPVIVHQDGSGLTVADALLMMKQ"
     gene            5221..6120
                     /locus_tag="VGC82_12610"
     CDS             5221..6120
                     /locus_tag="VGC82_12610"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0424155.1"
                     /translation="MLTIEPTGAILGATITGIDLSRPIPESDFGRILLALGRHGVLRF
                     PNQDLDVGDVKRFSELFGEIQGNPIRSMGATRPFPEVGILSNVKENGEYIGSPDAGQD
                     WHTDMSYRETMGFVNVLYGIRIPRRDGKALGGTEFANMHAAYDALPENIRSRLGSMTV
                     THDFEKFWEHMRRDKASLRPAMTDEQRKRRPPVVHPLFLTHPITRRKVLYCNPGYAVR
                     INELSQADSDQMLDMIFRHQLEMRFRYTHVWNENDLLIWDHLGTIHRAIADYGPDEIR
                     LMRRCQVMATKVFNADFLRPALV"
     gene            6363..7103
                     /gene="aqpZ"
                     /locus_tag="VGC82_12615"
     CDS             6363..7103
                     /gene="aqpZ"
                     /locus_tag="VGC82_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HEY0424156.1"
                     /translation="MRDDMARRISAEFFGTFWLTFGGCGAAVLAAGFPAVGIGLLGVS
                     LAFGLTVLTMAYAVGHISGGHFNPAVTLGLWSAGRCANKHVLPYIVAQVVGAIVAAGV
                     LWVIASGKPDWVPAGFAANGYGALSPGKYGLTACLITELLMTFFFLFIIVGTTSKGAA
                     SGFAGIPIGLALTLIHLVSIPVTNTSVNPARSTGAALFAGSEYIGQLWLFWLAPIVGG
                     MVAGALARWMYEPDTLIEAIVVEERQAA"
     gene            7162..>7792
                     /locus_tag="VGC82_12620"
     CDS             7162..>7792
                     /locus_tag="VGC82_12620"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0424157.1"
                     /translation="MRWVRFSKDDAVSYGSLEGDLIQEIRGEPWGDATPTGRTYARAE
                     IKLEVPVIPRTFYCAGINYAAHIREMAHKRGVEPVFPEKADIGYRANNALIADGEPVV
                     IPHHAPPKVNYEGELVVVIGKQAKHLTEDNAMSCVFGYTIGNDVSERTWQRGDRTFWR
                     GKNTDTFKPMGPSIETAVDLDAMETVIRVNGREDLRFKTNDMIFGIAHYI"
BASE COUNT         1397 a         2372 c         2575 g         1448 t
ORIGIN      
        1 tccgccccgc gcgggctgga tcaggttttt ggcggcatgg ctgtccagat ggtcccccgg
       61 cgtcggccaa gagctatttt caggtcgtaa gaattctcct cgttaaaaat tctcctggta
      121 tggcagggtc aaagtctgac gtggtcggtt accatggggt tgcttgtccg gcactcgtgt
      181 caggaagctg gaggtcgcat tgaccacgat ggtttggccg cttcgccgga cctgtttggc
      241 tgttgccgcg ctgacgatcg cgccgatgcc ggcacgagcg gagttcttcg acggtgcgcg
      301 gcggaccttc cagactgata ttccgcattt ctttcaagac gatatcccct gcgcctttgg
      361 cgggcagccg acgagccata ccaagacatc ctgcaaatcg tccgaccatt cggcgaagcg
      421 cgcgcccgcc agaactggtg atcgcgacgc agtttcaccc gccaaggccg acgacgcgca
      481 caacgtaccg gccaagtcca accgctagaa cccgcgggca ggccccggtt tccgccgaag
      541 cccaggtcgc ctgtcgtcga gttgacctcc cggcccgttc agggtcccaa gcgagccggc
      601 caaagcggcc atgcggcgcc ttgcggccgc ccggccgcca cctgagactt gccccttcgc
      661 tgcctcggga taacaatcgg cccgggtgga aaagggtcag gaaccatgaa cttcgaattc
      721 tcggacgagt tgcttcagct ccgagagcag gcgcgccggt tcctgaccga tcgctgttca
      781 ccgtcggtgc cgcggcgtat tcttgatacc gacgagccct acgccgccga tttatggcag
      841 gctgttgccg agatgggctg gaccggggcg gcgattcccg aggagtacgg cggtgtcgga
      901 ctcggccgcc tcgcggtttg tgtgctggcg gaggaactcg gccgggcggt ggcgccgatc
      961 ccgttttcgt cgtcggttta tttggcgacc gaagcgatca tgctgttcgg gtcggagcag
     1021 cagaaacagg cctggctgcc caagctggcc acgggcgaga tcattgggac gtttgcactg
     1081 gccgagcggc cgggatcgtc cgatccccat aaactgaccg catccgtaaa cgccggccgc
     1141 ctgagcggga ccaagctcgc ggtcccggac ggcgacatcg ccgacctcgc gatcgtggcg
     1201 gtcaacggtg gcaacggcgc ctggctgcat ctggtggatt tgaagggtgc cggggtgacg
     1261 cgggggacgg ttgtcacgtt cgacccgacg cgctcgcacg cgcgcatcga attttcggac
     1321 gcccgggcgg aaccactgcc ggacagcctt ggcgccgaga cggtacgcac gctgctcgat
     1381 tacgccgctg tgccgatggc gttcgagcag gtcggtgggg cgcaagccgc gctcgacatg
     1441 gccaatgcgt atgccaagga acgctttgcc tttggccggc cgatcggatc gtttcagggc
     1501 atcaagcaca agctcgcgga catgtacatc caggtcgagt tggcccgctc caacgcttat
     1561 tacggcgcgt gggcggttca caccggtgcg gccgaattgc cgatcgccgc gtcggtcgca
     1621 cgtatcgcgg cgtccgaggc cggctggttc gtgaccaagg aaaacatcca gacccacggc
     1681 ggcatgggct tcacctggca gatggattgt cacctgtatt accggcgcgc caagctgctc
     1741 agcctggcgt taggcggggc gcgcgagtgg aagcaccggc tggtgcgcga actgcgtgcc
     1801 cgcaacacca ccgatctggc agcggcatag gagcgcgacg atggatttcg aagacactcc
     1861 ggacgaagcc gcgttccgcg ccgaagcccg cgcttttctc gacgccaacg ccgaacgcag
     1921 gaaaccgggc gaggtggaag gctatcgccg cggccaggac gcgcctggtg ccatgcagcg
     1981 cgcgaaggat tttcaacgtc gcaaatatga cgctggattt gtcggcatcc attggcccac
     2041 cgagtggggc gggcggggcg gcacgccgat ccagcatgtc atctacaatc aagaagaggc
     2101 caaatacgat gtcctgacgg gcctcttcgg catcggcatc aatcttgcgc agccaacgat
     2161 ctgcacctgg ggcacgccgg aacagcggga tcgtttcgtc aagccggcta tgcgggcgga
     2221 cgaggtctgg tgccagttgt tcagcgaacc cgacggcgga tcggatctcg cggcgctgcg
     2281 cacccgagcc gaacgggacg gcgacgaatg ggtcgtcaac ggccagaaga tctggaattc
     2341 cggggcgcat tacagcgact tcggcatact ggtcgcgcgc agcaacccgg atgtgccgaa
     2401 gcatgagggg ctgacgttct tcatcctgga tatgaagacg ccgggcatcg aaatccggcc
     2461 gatccgccag gtttcgggat cgtcgcattt caacgaggta ttcttcacca acgtccgcat
     2521 tcccgacagt cgccgcgtcg gacccgtcgg tgcagggtgg aaagtcgcgc tgaccacgct
     2581 gatgaacgaa cgccacggca tgcgcgacgc ccccggcccg gatgtggccg aactcttccg
     2641 gctggcgcag tcgatcgaac tggaagatgg gccggccatc gatgattcgg cggtacagga
     2701 aaaattggcg gagttctaca cccgctccca aggcctgaaa tataccaagt tccgcggcat
     2761 cacggcgttg tcgaaggggc aaacgccggg gccggaatcg tcgatcatca aggtcgtcgc
     2821 ggcaaacaag ctgcaggaca ttctgtccta cgggttggat atgattggca tggccggtcc
     2881 gacgatgggc gacgcgatgc cggttgcagg catcttcgag gaggccctgc tttactcgcc
     2941 ggcattgcga ctggccggcg gcaccgatga aatcatgaag aacatcatcg ccgaacgtgt
     3001 gctgggcctg ccgcaggatc cgcgcgccga taaagggttg ccgttccgtg acattcccac
     3061 gggagtgcgc aagtgacttc agtcaacgcg acgacacaca aactccatag cttcttctgg
     3121 ccggaatcga ttgccgtgct tggcgcctcg ccggacctgc accgcatccg cgggcggttg
     3181 ctgcaccaac tccgggagaa cggctttccc ggccgcattc tgccgatcaa tcccagctac
     3241 caggagatcg aggggctggc ctgctatcca tcgatcggag cggcgggtgg cggcgggatc
     3301 gacctcgcgc tgatcgccat tcccgccgcc ggcgtggcgg cggccgttga agagtgtgcg
     3361 cgggccggcg tcaagaatac cctgatcatc agttccggtt tcgccgagga aggcggggcc
     3421 gccggcgaca tgcaggccga acttctggcg gtgacgcaac ggactggaat tcgcgcctgt
     3481 ggccccaact gcgagggcta ctacaatgcg ctcgggcgtg tcgccacgac gttcagccca
     3541 accgtcgaaa ccagggaaga tgacggccgg gtcctggtat cggacaagcg gatcggggtg
     3601 atcgcgcaat cgggcgggat cggtttcgcg ctgttcaacc ggggcaaagc cgccggcctg
     3661 ggcttcagct atgtcatctc caccggcaac gaagccgacc tgggcatggc agacttcctg
     3721 gactacatga tcgaggatcc gcacacccac gccgtcatgc tgttctgcga agccgtgcgc
     3781 aacggtcccg gcttcgtcgc ggccttggag aaagcgcgcc gcctgggcaa gccagtcatc
     3841 gccatcaagg tcggccggtc agacgccggc acccgggcga cggcgtccca tacagcctcg
     3901 ctgtccggat cttatgccgc ttaccacgcc gtgttcggac gctacggcgt gatcgaagcc
     3961 gaggacgccg acgaagcagt ggcgatcgcc ggcctcgtgc tgacctgccc gctgccgaag
     4021 ggcaggcggg ccgggatcat caccgtctcg ggcggcggcg gagcctggat ggcggacaca
     4081 ttgtccgcgc atgggctgat cgtgccgaat ttgtcggcgg ggtcgcagtc ggccctgcgc
     4141 ccgctgatgc cgtcctatgg ggcggcaggg aacccggtcg atgtcaccgc ccaaggctcg
     4201 aacaccggtc cagcgatgat gaccgtcatg gaacatctcg ccggatcgga cgagatcgac
     4261 atcgtcgtgc tggtcacgtc cctggccagc gaggtgcggg cgtcactgga tgccgcgcgc
     4321 atccgggcgg tggcggcaag ctgcggcaag ccgatgacgg tctggagcta caccttgccc
     4381 tcggcgttcg ggcgcgaggc cgccgcgggc tgtggtttgt ttgtggacag caaccttcgc
     4441 aacatcggcg tcgcgatggg caagcttgcc ggctacgcgg aagcgttgca gcgacaattg
     4501 ccggaaccgt tcgcgccggt gtcgggtcgg ctgtatcccg gcctgccggc ggtggtgccg
     4561 gagtatctgg ccaagcaggt gctggccgcg tggctgcccg acacacggga aaagctcgtg
     4621 acatcggccg aagcggcggc ggatgcggct cagcacctgg gctttccggc ggtcctgaag
     4681 gtccagtcgc ctgacctgcc gcataagacc gaggcaggtg gggtgcggtt gaatctggtg
     4741 gatcgggacg cggtgataaa cgcctacacg gcgatggtgg cggatgtcgg gcgtcatgcg
     4801 cccggggcat cgatcgacgg cgtcctggtg cagcggatgg caccaaaggg tcatgagctg
     4861 gtcatcggca tagtcgatga tccgaccttc ggccccatca tgatgctggg ttttggcggc
     4921 accacggtgg agctgttcgg cgacgtggtg catgcacccg ccccggtgga cgaggcggaa
     4981 gccacccgca tgatcctgtc gctgaaatcc gcccgtttgc tgaccggctt tcgtggcgcg
     5041 aagccaatcg atctggctcc cgttgctcgg ttggtcgctt cgctgtcgcg tgcggcgctg
     5101 acattgcgcg accaggtgcg cgaattcgag ctgaacccgg tcatcgtgca tcaagacggt
     5161 tcgggactga ctgtcgccga cgccttgttg atgatgaaac agtaaaggag gtcctgcgtg
     5221 atgctgacca tcgagccgac cggcgcgatc ctcggtgcca cgatcaccgg catcgatctg
     5281 tcgcggccaa ttccggaatc cgatttcggc cgtatcctgc tcgcccttgg ccggcacggc
     5341 gtgctgcgct tcccgaacca ggatctggac gttggcgacg tcaagcggtt ctccgagctg
     5401 tttggcgaga tccagggcaa tcccatccgg tcgatgggtg cgacgcggcc gtttcccgag
     5461 gtcggcattc tttccaacgt caaggagaac ggcgagtata tcggcagtcc ggatgccggg
     5521 caggactggc acaccgacat gtcctatcgg gaaacgatgg ggttcgtgaa cgtcttgtat
     5581 ggcatccgca ttcctcgccg ggacggcaag gcgttgggcg gcaccgagtt cgcgaacatg
     5641 cacgcggcgt acgacgcact gccggaaaac atcaggagcc gtctgggcag catgacggtc
     5701 acccacgatt tcgagaaatt ctgggagcat atgcgtcggg acaaggccag cctccggccg
     5761 gcgatgacgg acgagcagcg gaagcggcgc ccgcccgtgg tgcatccatt gttcctgacg
     5821 catccgatca cccgccggaa agtgctgtac tgcaacccgg gttatgccgt gcgcatcaat
     5881 gaactgtcgc aagccgacag cgaccagatg ctggacatga ttttccgcca ccagttggaa
     5941 atgcgtttcc gctacaccca cgtttggaac gagaacgatc tattgatctg ggatcatctt
     6001 gggaccatcc accgtgccat cgccgattat ggtccggacg aaattcgcct gatgcgccgc
     6061 tgccaggtca tggccaccaa ggtgttcaat gccgactttc tgcgcccggc acttgtttga
     6121 ccgcatgatt cactcgcttc ggacgttccg ccgctccgga cgttccgccc ggcgtgatca
     6181 tctgcagagg ccgcggaggc atgaaacggt gcaccggtaa catcacgagt ggtgggctac
     6241 ttttttgatg caattcattt caaacaaacc gcaaatagtc gctgtcggac gctttgtccg
     6301 gaatccgatc gcctggacct gatcgttcag acgcaaagcg tcctatgggg gtggaggttc
     6361 atatgcggga cgatatggca cggcggatat cggcggaatt ttttggcaca ttctggctga
     6421 cgttcggcgg atgcggcgca gcggtgctgg cggcggggtt cccggcggtg ggtatcggac
     6481 ttctaggggt gtcgttggcc tttggcctga ccgtgctgac catggcttat gccgtcggcc
     6541 atatctccgg tggccatttc aatccggccg tgacgttggg cctgtggtcc gccggccggt
     6601 gtgccaacaa gcatgtgctg ccctacatcg tggcgcaggt cgtcggcgcc atcgtggccg
     6661 ccggggttct atgggtgatc gcatcgggca agccggattg ggtgcccgcc ggtttcgcgg
     6721 caaacgggta cggcgcactc agtccgggca agtatggttt gaccgcctgc ctgatcaccg
     6781 aactgctgat gactttcttc tttctgttca tcattgtcgg gacgacctca aagggcgcgg
     6841 catccggatt tgccggcatc ccgatcggcc tggctttgac gctgatccac ctggtgtcga
     6901 tcccggtcac caatacctcg gtcaacccgg cacgaagcac tggggcagcc ctttttgccg
     6961 gcagcgagta tatcggccaa ttgtggctgt tctggctggc gccgatcgtg ggcggaatgg
     7021 tcgccggcgc gttggcacgc tggatgtacg agccggacac gctgatcgag gcgatcgtcg
     7081 tggaggaacg ccaggcggcg taagcttgcc agaccggttg ggcgggagcg atcatggcgg
     7141 accaaaatag ggggatcggc catgcgctgg gtgcgtttca gtaaagacga cgcggtttcc
     7201 tatggctcgc tggaaggcga tctgatccag gaaatccgcg gcgagccgtg gggcgatgcc
     7261 accccaactg gccgcaccta tgcgcgtgcg gaaatcaaac tggaagtgcc ggtgataccc
     7321 cgcacctttt actgcgccgg catcaactac gcggcgcata tccgcgagat ggcccataag
     7381 cggggcgtgg agcctgtgtt tccggaaaaa gccgacatcg gataccgggc gaacaacgct
     7441 ttgatcgccg atggcgagcc ggtggtgatc ccgcatcatg cgccgcctaa ggtgaattat
     7501 gagggcgaac tggtcgtggt catcggcaag caggccaagc acctgaccga ggacaacgcg
     7561 atgtcctgcg tgttcggcta tacgatcggc aacgatgtta gcgagcgcac ctggcagcgc
     7621 ggcgaccgca cgttctggcg tggcaagaac accgacacgt tcaagccgat ggggccatcg
     7681 atagaaaccg ccgtcgacct ggatgcgatg gagaccgtta tccgggtcaa cggccgggaa
     7741 gatttacgtt tcaaaaccaa cgacatgatc tttggcatcg cccactacat tg
//
LOCUS       DASWQN010000743         2906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2183174, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000743 DASWQN010000000
VERSION     DASWQN010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183174"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VGC82_12625"
     CDS             <1..194
                     /locus_tag="VGC82_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424158.1"
                     /translation="NSASGFTFVGSTCLDRPRGSDFDPDRSDVDLMVTYRPGHHPGVA
                     AYQDLRDALAKLFGRRSIW"
     gene            complement(663..739)
                     /locus_tag="VGC82_12630"
     tRNA            complement(663..739)
                     /locus_tag="VGC82_12630"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(703..705),aa:Pro,seq:ggg)
     gene            858..2441
                     /locus_tag="VGC82_12635"
     CDS             858..2441
                     /locus_tag="VGC82_12635"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HEY0424159.1"
                     /translation="MIAFAALLERLVFTPSRNAKIALLRRYFATQPDPDRGIGLAAIA
                     GDLSFTAAKPGLIRELAAARTDPVLFDWSYDYVGDLAETVALIWPAAPVSTDPPTLAE
                     VVEALETEPKAALPALVAGWLDCADSSTRLALLKLITGGLRVGASSRLAKIALAEIGG
                     VSADDVEEVWHGLQAPYLPLFAWLEGRGPKPDPADAPVFRPPMLAHPLEAADLAALLP
                     EDWRAEWKWDGIRVQMVATTGGRRLYSRGADDISGAFPEIVAAMQFDAVLDGELLVIR
                     DGVVAPFADLQQRLNRKVVSAKMIERFPVGVRVYDLLFDGTEDLRGLPFDQRRLRLEA
                     WLARTNPARMDLSPLIRFSSLPELMVLRDGARAASIEGLMLKRGDSAYLPGRVKGQWW
                     KWKRDPLTIDAVLMYAQRGHGKRSSFYSDYTFGLWRGNELVPVGKAYFGFTDQQLTFL
                     DRWIRNNTTARFGPVREVEKSMVLEVAFDAAQLSNRHKSGVAMRFPRLARIRTDKPAA
                     EADRLETLMALVEDREVHG"
     gene            complement(2504..>2906)
                     /locus_tag="VGC82_12640"
     CDS             complement(2504..>2906)
                     /locus_tag="VGC82_12640"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEY0424160.1"
                     /translation="RLFGQRWNPPGLPAIYTGTSFAVCLLEILVHANRKTPPSAARSV
                     KAVVPDNVSREMFDLALHPGWDNKRDPSIAQAFGRTWIREKRSALLLVPSAVTAGRDT
                     NVVVNPDHPDAVRILVGPETELGLDRRLFGA"
BASE COUNT          493 a          876 c          947 g          590 t
ORIGIN      
        1 cgaactctgc cagcggtttc acgttcgtcg gctcgacgtg tttggatcgg ccgcgcggtt
       61 ccgacttcga tccggaccgt tcagatgttg acctgatggt cacttatcgt cccggccacc
      121 atcccggtgt cgccgcctac caggatctcc gagacgcttt ggcgaagctg ttcggccgcc
      181 ggtcgatctg gtgatggaaa ccgcggctga gaaccccttc atccgggcgg caatcggacg
      241 gtcgcggcag gcgatctatg caacgtgacc catgtgcatg gttgtggacg tttgtcaggc
      301 cgccgatatc atcgccgcgt tgttcagggt cgcgtttgtt cagggtcgcg attttgataa
      361 ctacatcgcc gaccggatgg cttcgttctg cagtggaacg ccaattcgaa atcgccggtg
      421 aggcactaaa gcggcttcct cgggaggctc cggaactcgc cgcgcgactg cccgatcttc
      481 ggttcgcgaa cgccatgcgc cgatccatgc tatcgcgaac tggacgatgg gactgttcgg
      541 cagaccatcc acgacgttct ccctgatgtc cggacgcaag ttgctgcttg ctgagcgggc
      601 tgggcgatca ccgtaacaaa ttgtcatgta gccgtcataa tgatgcgggg aaacggtaac
      661 gttggtcgga gcggcgggat tcgaacccac gacccccagt cccccagact gatgcgctac
      721 caggctgcgc tacgctccga ccgcggggat gtgtcgttta acagcctcgt ggtccggccg
      781 caatgcgcct gctcgcctcc gacactgctc gctaccgaca ctggcgctcc gccgcgatca
      841 gcccaacata ccgaagtatg atagccttcg ccgccctgct ggagcgtctg gtcttcacgc
      901 cgtcccgcaa tgccaaaatc gcgttgctga ggcgttactt cgccacacag ccggatccgg
      961 accgcggcat cggccttgcg gcgatcgccg gtgacctgtc cttcaccgcg gcaaaaccag
     1021 gattgatccg cgaactggcg gcggctcgga ccgatcctgt cctgttcgac tggtcgtacg
     1081 actacgtcgg agacctggct gaaaccgtgg ccttgatctg gcctgccgcg ccggtctcca
     1141 cagacccccc aacgcttgcc gaggtggtgg aggccttgga aaccgagccg aaggctgcct
     1201 tgccggcttt ggtggcgggg tggctcgatt gcgccgactc ctcgactcgt cttgcgctgt
     1261 tgaagctgat cacgggcggc ctgcgcgtag gggcctccag ccggctggcg aaaatagcac
     1321 tggctgaaat tggcggcgtc agcgccgacg atgtggaaga agtctggcat ggcctgcagg
     1381 ctccctatct gccgttgttt gcttggctgg agggcagggg gccgaagccg gatcccgccg
     1441 atgctccggt ctttcgtccc ccgatgctgg cccatcccct ggaagcggcc gatcttgccg
     1501 ccctgctgcc cgaggattgg cgggcggaat ggaaatggga tggcatccgg gtgcagatgg
     1561 tcgccacgac cggaggccgg aggctgtatt cacgcggtgc ggatgatatc tccggcgcct
     1621 tcccggaaat cgtcgcggcg atgcaattcg atgctgttct ggatggtgaa ctgctggtga
     1681 tccgtgacgg ggttgtcgcc ccgttcgccg acctgcagca acggctgaac cgcaaggtcg
     1741 tttcggcgaa gatgatcgag cgttttccgg tcggcgttcg ggtttacgac ttgctgttcg
     1801 acggcaccga ggatctgcga ggattgccat tcgatcagcg ccgtctgcgc ctcgaagctt
     1861 ggctggcgcg caccaatccg gccaggatgg atctctcgcc gctgatccgg ttttcgtcgt
     1921 tgccggaatt gatggtgctg cgcgatggtg cgcgagccgc ctcgatcgag ggcctgatgc
     1981 tgaagcgcgg cgacagcgcc tatctgccgg gcagggtcaa gggccagtgg tggaagtgga
     2041 agcgtgatcc gctgacgatc gacgcggttt tgatgtatgc gcaacgtggt cacggcaagc
     2101 gcagcagttt ctactccgac tatacttttg ggctttggcg gggcaatgag ttggtgcccg
     2161 tcggcaaggc ctatttcggc ttcaccgacc aacagctcac ctttctggac cgatggatcc
     2221 gcaacaacac cacggcccgg tttggaccgg tccgcgaggt ggagaagtcg atggtgctgg
     2281 aggtggcgtt cgacgcggcc cagctatcga accgtcataa gtccggcgtc gcgatgcgat
     2341 ttccccggct ggcccgaatc cgcaccgaca agccggccgc cgaggcggat cggctggaga
     2401 cgctgatggc tttggtcgaa gaccgcgaag ttcacggttg aatgccagca acgaggtttt
     2461 gagatggggc attctgttta tccaaggcct tccgccggga gcgtcaggct ccgaatagcc
     2521 gccggtcgag gcccagctct gtctccggcc ccaccaaaat tcgcactgcg tcagggtggt
     2581 cgggattgac gacgacattt gtgtcgcgtc cggcggtgac ggcggaggga acaagcagga
     2641 gagcggatcg tttttccctg atccaggttc ggccgaaggc ttgggcgatc gacgggtcgc
     2701 gcttgttgtc ccaccctgga tgcaaggcca gatcgaacat ctcgcgagaa acattgtcgg
     2761 gaacaacggc tttaacggat cgcgcggcgc tcggcggcgt cttacggttg gcatgcacga
     2821 ggatctccag aaggcagacg gcgaagctcg tgcccgtgta gatcgccggc aggcctggcg
     2881 ggttccaacg ctgaccaaac aggcga
//
LOCUS       DASWQN010000744         3475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1919343, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000744 DASWQN010000000
VERSION     DASWQN010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1919343"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..905)
                     /locus_tag="VGC82_12645"
     CDS             complement(156..905)
                     /locus_tag="VGC82_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0424161.1"
                     /translation="MIELSSAPASLPPGRRVYAIGDIHGCARQLANLHALIRRDLEQR
                     PVASALLLHIGDYVDRGADTSGVIAMLVDGCPIPGMEMVNLMGNHESTMLDALAGERA
                     AGTDWLFAGGKAALQSYGIDPDGPRDLWPKLIPRSHQAFLRDLVLMHREGGYAFIHAG
                     VRPGIPLEDQARDDLLRSRQPFLYSEADFGAVVVHGHTPVKAPVVRRNRIAIDTGAVF
                     GGKMTCLVLETGTMGFLTADPVDAPVSIAHR"
     gene            complement(964..2118)
                     /locus_tag="VGC82_12650"
     CDS             complement(964..2118)
                     /locus_tag="VGC82_12650"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEY0424162.1"
                     /translation="MTGTPTPFTLAIPDQDIADLRARLALTRFPDQAPDAPWAFGTDL
                     GYMKSLVPYWRDRFDWRAQEAALNAFPQFKVRMPDVDLHYLHVLGQGPSPTPLLLMHG
                     WPGSVFEFLDIIPRLTDPARFGGDPADAFTVIAPSLPGYGLSFTQGQKRFNIEAIADC
                     LNALMTDVLGYRRYAAQGGDYGAITSSRLGYAHPDNLIGIHINLLSVRRDGPAPDHPT
                     DEERRYFDELALWLKEETGYQWIQGTKPQTLAYGLTDSPAGLAAWIAEKFRTWTDCDG
                     DPESAVSRDHLLANISFYWFTGAIGSSFWPYYARMHGPWPIPRGGTVDVPMGYCAFPK
                     EIIRPPRSVAQQMYTNILRWTEMPKGGHFAAMEQPAALAEEIRAFFRPLR"
     gene            complement(2208..2579)
                     /locus_tag="VGC82_12655"
     CDS             complement(2208..2579)
                     /locus_tag="VGC82_12655"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0424163.1"
                     /translation="MAGNGQKIIELDRQRMAAMAAKDVAALNALLADDLIYTHSSARM
                     DTKKTLVGAMESGATVYTAIEPSEVVAQDLGDAVVLTGVAAISVNSGGNANSFRVRFT
                     DVYANRNGQWQMVTWQSTRLA"
     gene            2683..3276
                     /locus_tag="VGC82_12660"
     CDS             2683..3276
                     /locus_tag="VGC82_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY0424164.1"
                     /translation="MLDHLKANNRRWAARKVAADAGFFKRLEGQQAPEYLWIGCSDSR
                     VPANEIVDLDPGELFVHRNVANLAPPQDANYLSVLQFAVDVIRVKHIIVVGHYGCGGI
                     AAAVDGRRRGLVDHWLHPIREVFNENRPLLEAIADQSERLNRLCELNVMRQVRNVSSD
                     VFVQDAWARGQDLSVHGWVYSLGNGLVTDLDVTVNKP"
BASE COUNT          635 a         1092 c         1128 g          620 t
ORIGIN      
        1 catgggtgtg cgtgtgtctc cggggggcgc tagctagcag gaagcgcggg ccggggaaac
       61 atttgcttct ccccgcttcg attatcgaca ggtgcccgat tatcgatagg tgcccaaact
      121 gtcggcaggc tcccaactgt cggagggctc ccgaactatc ggtgggctat cgataccggc
      181 gcgtcaaccg gatcggcggt caggaagccc atcgtgccgg tttcgagcac aagacaggtc
      241 atcttgccgc caaacaccgc gccagtgtcg atggcaatcc ggttgcggcg gaccaccggc
      301 gcttttaccg gcgtgtggcc atgcaccacc acggccccaa aatccgcctc ggaatacaga
      361 aaaggctgcc gcgagcgcaa caggtcgtcg cgcgcctggt cctcgagcgg aatgccggga
      421 cgaacgccgg cgtggatgaa ggcgtagccg ccctcccggt gcatcagcac caggtcgcgc
      481 aggaacgcct gatggctccg tgggatcagt ttgggccaca ggtcgcgtgg accgtccggg
      541 tcgatgccgt agctttgcaa cgccgctttg ccgccggcga acagccagtc ggtgccggcg
      601 gcacgttcgc ccgccaaggc atccagcatg gtgctttcgt ggttgcccat caggttgacc
      661 atctccatcc ccgggatcgg gcagccatcg accagcatgg cgatcacgcc ggacgtgtcc
      721 gcacctcggt ccacataatc gccgatatgc agcagaaggg cggaggcaac gggccgttgc
      781 tccagatccc gcctgatcag cgcgtgcagg ttggccaact gccgcgcaca gccgtggatg
      841 tcaccaatgg catagacacg ccgcccgggc ggcagcgagg cgggcgcgga actgagttcg
      901 atcattgcgc atcggggtta caggcccatg gcgattgaat caacctcacg ggcctctgct
      961 tgcctacctg agaggacgga aaaacgcccg gatctcctcc gcgagagccg ctggctgctc
     1021 catcgcagcg aaatggccgc ctttcggcat ctccgtccaa cgcaggatgt tggtgtacat
     1081 ctgctgcgcg accgaacggg gcggccggat gatttccttt ggaaacgcgc agtagcccat
     1141 cggcacatcg acggtgccgc ctcggggaat gggccacggg ccgtgcatgc gcgcgtagta
     1201 cggccagaac gacgagccga tggcgccggt gaaccagtag aacgagatgt ttgccagcag
     1261 atggtcgcgg ctgaccgccg actccgggtc gccgtcgcag tcggtccagg tgcggaactt
     1321 ctcggcgatc caggcggcta ggccagccgg cgagtcggtc aggccgtagg ccagagtctg
     1381 cggcttggtg ccctggatcc actggtaacc agtctcctcc ttcagccaca gcgccagctc
     1441 gtcgaagtag cggcgttctt cgtcggtcgg atggtccggc gccgggccgt cccgccggac
     1501 gctcagcagg ttgatgtgga tgccgatcag attgtccgga tgtgcgtagc ccaggcggga
     1561 agaggtgatg gcaccgtagt cgccgccctg cgccgcgtag cgccggtagc cgaggacatc
     1621 ggtcatcagg gcgttcaaac agtcggcgat ggcctcgatg ttgaaacgct tctgcccttg
     1681 ggtgaaggac aggccgtagc cgggcagcga aggcgcaatc accgtgaacg cgtccgccgg
     1741 gtcgccaccg aagcgggcgg gatccgtcag gcggggaatg atgtccagga actcgaacac
     1801 cgatcctggc cagccatgca tcagcagcag cggggtgggc gacggcccct gccccaacac
     1861 atgtaggtaa tgcagatcga catccggcat ccgcactttg aactgcggaa aggcgttcag
     1921 tgccgcctcc tgcgcccgcc agtcgaaccg atccctccag tacggtacga ggctcttcat
     1981 gtagccgaga tcggtgccga acgcccacgg cgcatccggc gcctgatccg gaaaccgggt
     2041 cagcgcgagg cgcgcgcgca gatccgcaat gtcctggtcc gggatcgcga gcgtgaatgg
     2101 cgtcggtgta ccggtcattc tgaacgtccc ctgaaattgc cacccatcaa gatgacaatc
     2161 caggcgaacc ggtcaagcca ccggcttgcc ggcctgaccg cctgggccta cgccaatcgc
     2221 gtgctctgcc aggtcaccat ctgccactgg ccgttcctgt tggcataaac atcggtgaaa
     2281 cgcacgcgga acgagttcgc gttgccgccg gagttgacgc tgatcgcggc gacccctgtc
     2341 agcaccaccg catcgccgag atcctgtgcg acgacctcgg acggctcgat ggcagtataa
     2401 acggtcgccc ccgactccat ggcgccgacc agcgtcttct tggtgtccat ccgagcagaa
     2461 gaatgcgtgt agatcaggtc gtcggcgagc agcgcattga gtgccgcgac gtctttcgcc
     2521 gccatcgccg ccatgcgctg acgatcgagt tcgatgatct tttgcccgtt tccagccatt
     2581 atgaacgtcc ctccgttaat cttgtcaggt ctcgccgcat cgtaggcaga caacggacgc
     2641 ttggccagat atcctgcccg ccagcagctt gcgggagttc gggtgctcga tcatttgaaa
     2701 gcgaacaatc gccggtgggc ggccaggaag gtcgccgcgg acgccgggtt cttcaaacgg
     2761 ctggaaggcc agcaggcgcc ggaatatctt tggatcggct gctccgacag ccgggtgccg
     2821 gccaacgaaa tcgtcgatct cgatcccgga gagctgtttg tccatcgcaa tgtcgccaac
     2881 ctggcgccac cgcaggatgc gaactacctg tctgtgctgc agttcgccgt cgatgtgata
     2941 agggtaaagc acatcatcgt cgtcggtcac tacggctgtg gtggcatcgc ggcggcggtg
     3001 gatggcaggc ggcggggcct ggtggatcac tggctgcatc cgatccgcga ggtgttcaac
     3061 gaaaaccggc cgttgctgga agccatcgcc gaccagtcgg agcggcttaa ccgcttgtgc
     3121 gagctgaatg tcatgcggca ggtgcgcaac gtgtcgtccg acgtgttcgt gcaggacgcg
     3181 tgggcgcgcg gacaggacct cagcgtgcat ggctgggtct attcactggg caacgggctg
     3241 gtgacggatc tggacgttac ggtaaacaag ccctgagttt gctggtaacc gcttccagtc
     3301 ggccaatccc ggctgatacc ggtccacatg aaataagcgc ttcaagcccc gtgtccaaac
     3361 gcgttacgac ctgcggaggc ctgcacaggc gtggcggctg actatagatt gtggcgtagc
     3421 cgcgcttggg gcggcaccaa atattgtgtc atggccgtgc ctagcgcggc caccc
//
LOCUS       DASWQN010000745         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_72506, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000745 DASWQN010000000
VERSION     DASWQN010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72506"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VGC82_12665"
     CDS             <1..628
                     /locus_tag="VGC82_12665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0424165.1"
                     /translation="HGAGRGMMVATQSLRDELAARGFRNIRPWSRGVDLDLFKPYPKT
                     EFTHLPAPIFIYVGRVAVEKNIGAFLKLDLPGSKVVVGGGPQLASLRREYPNTTFTGP
                     RHGEALARAYSGADVFVFPSLTDTFGLVILEALACGTPVAACPVTGPKDVLIGNDGKI
                     GAIDADLQRAALDALTADRAACRTHAERYSWRACAETFLSHLVPINTR"
     gene            complement(708..1340)
                     /locus_tag="VGC82_12670"
     CDS             complement(708..1340)
                     /locus_tag="VGC82_12670"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424166.1"
                     /translation="MGFAPKQLFSRENRYSTMVGVLFCLGALLGIWPSNVWLPTIQGQ
                     MLAHSGITGAAAVPFIGFGMTLFGIGGMLGQAAYGFIADAIGRRWANILYSTGTLVAG
                     MTLYVVLQDYSYYPFVLPFFGFSVFGIFAGLAVYLPELFPTHMRATAVSFCSGSGRVI
                     TAFGPLVAGLLVVPFGGNFARATAVITCFAVLSIVAMIIGRETKGDTLPH"
     gene            1339..1998
                     /locus_tag="VGC82_12675"
     CDS             1339..1998
                     /locus_tag="VGC82_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424167.1"
                     /translation="MNTRSSALIGLRHFQGLAAGVHRLEALRFLHAAPQREQDQRRDH
                     AGEQRVAPAVGADQRIELAAQDAAERRAGHHETEHPGTRQVGECLGDKGDADHELRAG
                     ADAGQKAANAEDERVRGKALQRREHRDHDHAQGQGADAADIVSDHPEGHAAKCPTEQA
                     DHAEQAADPPDVGDRRSTAQKLGQRRAQHQGKQVEVGIVDCPAEPREQQHLPLVTCDV
                     S"
     gene            2168..>2869
                     /locus_tag="VGC82_12680"
     CDS             2168..>2869
                     /locus_tag="VGC82_12680"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0424168.1"
                     /translation="MEEEATQVLAVFAATLDVDRMPERVLVQCKNLLLDALACAVAGH
                     AGEETPQVAAFATALAPGLGTSIIGGNQGSLTGATVLNAFLITAITMCDAHRTTLTHV
                     TPEVVPPALAIAERDNLSGRDLLAALAAGFEVTTRVGIGLDFAEFRGRGFHGPGILGP
                     FGAAAAVGRLLRFDAATMARGFGLAGSQAAGTFAAWGTPTVKFHQCRGALSGLMAALL
                     AQQGFLANPDFLTRPD"
BASE COUNT          548 a          933 c          930 g          458 t
ORIGIN      
        1 ccatggcgct ggccgcggga tgatggtggc cacgcagtcg ttgcgcgacg aactggccgc
       61 acgaggcttc cggaatatcc gcccatggtc gcgcggcgtc gatttggacc tgttcaagcc
      121 gtatccaaag accgagttca cgcatctgcc tgcgcctatt ttcatctatg tcggacgggt
      181 ggcggtggaa aagaacatcg gcgcgttcct gaagctcgat ctgcccggat cgaaagtcgt
      241 ggtgggcggc ggaccgcaac tggcatcgtt acgacgcgag tatcccaaca cgacattcac
      301 cggacccagg cacggcgaag cgctggcacg ggcctactcg ggcgccgatg tgttcgtgtt
      361 ccccagcctg accgacacgt tcgggctggt gatcctggag gctttggcct gcggcacgcc
      421 ggtggcagcc tgtccggtga ccggtccaaa ggatgtgctt atcggtaatg acggcaagat
      481 cggcgctatc gatgccgatc tacagcgcgc cgccctggat gcgctgaccg ccgaccgcgc
      541 cgcctgccgg acccacgctg aacggtattc ctggcgggca tgtgctgaga ccttcctgtc
      601 gcacttggtg cccatcaaca cccgataaac tggctggatg gtccgctccg agctgtccgc
      661 ttcaaacccg ctcctgactc cggcagacag tcagccaagg cacaagctca gtgcggcagt
      721 gtgtcgccct tcgtttcccg tccgattatc atggccacga ttgacagtac cgcgaagcac
      781 gtaatgaccg ccgtcgcacg ggcgaagttg ccgccgaacg gaaccaccag caaacctgcg
      841 accaatggtc cgaaggcggt gatcacgcgg cccgatccgc tgcagaacga aaccgccgtc
      901 gcgcgcatgt gcgtcgggaa caactccggc agatatacgg ccagtccggc aaaaatcccg
      961 aacaccgaaa agccgaagaa cggcagcacg aacggatagt agctgtaatc ctgcagcacc
     1021 acgtacaggg tcattccagc caccagtgtg ccagtactgt aaagtatatt ggcccaacgg
     1081 cgtccgatgg cgtcggcgat aaagccatag gccgcctgac ccagcatgcc gccaatgccg
     1141 aacagcgtca tgccgaagcc gatgaacggc acggcggcgg caccggtaat cccgctgtgg
     1201 gccagcatct ggccctggat cgtcggcagc cacacgttgc tgggccagat ccccagcagg
     1261 gcgcccaggc agaacagcac gcccaccatg gtgctgtaac ggttttcccg gctgaacaat
     1321 tgttttggcg cgaaccccat gaatacccga tcctcggcgc tgatcggatt gcgccacttt
     1381 cagggcctgg cggcgggcgt gcaccgcctg gaagcgctcc ggttcctcca tgccgcgccg
     1441 caacgcgagc aggatcagcg ccgggatcac gctggcgaac agcgggtagc gccagccgta
     1501 ggggccgacc aacgcataga gctggccgcc caggatgctg ccgaacgacg cgccggtcat
     1561 catgaaaccg agcatccggg cacgcggcag gtcggcgaat gcctcggcga caagggggat
     1621 gccgaccacg aactccgcgc cggtgccgac gccggacaga aagcggcaaa tgccgaagat
     1681 gaacgtgttc ggggaaaggc cttgcagcgc cgtgaacacc gcgaccatga ccacgctcag
     1741 ggccagggtg cggatgcggc cgatatagtc tccgaccacc ccgaaggcca tgccgccaaa
     1801 tgcccaaccg agcaggccga ccatgctgag caggccgccg acccgcccga tgtcggcgac
     1861 cgaaggagca ccgcccaaaa gctcggtcag cgccgggcgc aacaccaggg caaacaggtt
     1921 gaagtcggta ttgtcgattg cccagccgaa ccacgcgagc agcagcacct tccactggta
     1981 acctgtgatg tctcgtagcc agccacggct ggccgaggtc tcgccgctcg gcatttctgc
     2041 tcccctcggt tatttcccca ggcttgttcg gcggaccgtg tctgtcaatc tgcagcggac
     2101 cggatgcagt ggaccgaatc gggggatcgc cgcccggatc ggctgaacga gacagggaaa
     2161 gcgcagcatg gaggaagagg ccacacaggt actggcggtg ttcgcggcca cgctcgatgt
     2221 cgatcggatg ccggagcggg tgctggtcca atgcaagaac ctgctgctgg acgccttggc
     2281 atgtgccgtc gcaggacacg cgggcgagga aaccccgcag gtcgccgcgt tcgcaactgc
     2341 attggcgccg ggcctgggca ccagcatcat cggcggaaat caggggtccc tgaccggggc
     2401 gaccgtgctg aacgccttcc tgatcaccgc cataacgatg tgcgacgcac accgaaccac
     2461 gctgacacat gtgacgcccg aggtcgtccc gccggcactg gcgatcgccg aacgcgacaa
     2521 tctctccggc cgcgacctgt tggcggcgct ggcggcgggg ttcgaggtga cgacgcgcgt
     2581 cggcattggc ctggacttcg ccgagttccg cggtcgaggg tttcatggac caggcatcct
     2641 gggtccgttc ggggcggctg ccgcggtagg gcggttgctg aggtttgacg ccgcgacaat
     2701 ggcgcgcggt tttggactcg ccggcagcca ggcggcgggg acattcgcag cgtggggaac
     2761 gccgacggtc aagttccacc agtgccgggg cgcgctgtcc gggctgatgg cggccttgct
     2821 ggcgcagcag ggtttcctcg cgaatccgga ctttctgacg cgccccgat
//
LOCUS       DASWQN010000746         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_336557, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000746 DASWQN010000000
VERSION     DASWQN010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336557"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..>1734)
                     /locus_tag="VGC82_12685"
     CDS             complement(139..>1734)
                     /locus_tag="VGC82_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEY0424169.1"
                     /translation="VLCVADSTNLRLTIRLLLELKSTGRPLILVLNMFDIATRRGVSV
                     DVPRLSEALGVPVVTSIAVRKGGTGDLLRLTDEIAAQAQTPDRQNLWEPLTVAQLRAT
                     QREADRIIAAAVSLPARPDSWTGRIDAVVLHPVAGLVILAAILFVMFQAVFAWAQPLM
                     ELLSSAFAALGQLVHDTVPAGLLQSFLQNGVISGVGSVIVFLPQIVMIFLFILLLEDF
                     GYMARAAFLMDRIMGGAGLHGRAFIPLLSSFACAIPGIMATRVIDNRRDRLTTILVAP
                     LMTCSARIPVYTLIISAFIPAGQVWGGINLRGLVMFGLYAAGIISALGVSFVIKFFMW
                     RDYAPAPFMLELPDYKMPRARGIAIGVYTRAKMFLQRAGTTIFAMMVLIWFLASFPPP
                     PPGAEGPAINFSLAAMIGKALEPLLAPLGFNWQIAVALIPGMAAREVAVAALGTVYAI
                     EGGKEAADQIGQALASKWTLATALSLLAWYIFAPQCASTLAVIRRETGSWKWMAVTFG
                     YMLALAYAASLATFNIAVALGAG"
BASE COUNT          331 a          574 c          586 g          243 t
ORIGIN      
        1 cccgctccag gcgcggcgcg ggcgccgagg ctgaagccgc ggccgccgct gatgctcgca
       61 ccgccgttat acgagccgcc gcgatccggt ttctagcgcg ggcaggtacg ctccccgggt
      121 gattcaaaaa cgcggtgtct aaccggcgcc gagcgcaaca gcgatgttga aggtcgcaag
      181 gcttgcggca taggccagcg ccagcatgta gccgaaggtg accgccatcc acttccagct
      241 tccggtctcc cggcggatca cggcgagtgt cgacgcgcat tgcggggcga agatgtacca
      301 ggccagcaag gacagggcgg tcgccagcgt ccacttgctg gccaaggcct gtccgatctg
      361 atcggcggct tccttgccgc cctcgatcgc atagacggtg ccgagcgcgg caaccgccac
      421 ctcgcgcgcc gccatgccgg ggatcagcgc caccgcgatc tgccaattga agccgagcgg
      481 ggccagcaac ggctccaggg ctttgccgat catcgccgcc aggctgaagt tgatggcggg
      541 gccttccgcg ccgggcggcg gcggtggaaa cgacgccagg aaccatatca gcaccatcat
      601 cgcgaagatc gtggtgccgg cccgctgcag gaacattttg gcccgcgtgt agacgccgat
      661 ggcgatgccg cgggcgcgcg gcatcttgta gtcgggcagc tccagcatga acggcgccgg
      721 cgcatagtcg cgccacatga agaacttgat cacgaacgag acgccgagcg cgctgatgat
      781 gccggcggca tagaggccga acatcaccag cccgcgcaga ttgatgccgc cccacacctg
      841 cccagcgggg atgaaggcgg aaatgatcag cgtgtagacc gggatccgcg ccgaacaggt
      901 catcagcggc gccaccagga tggtggtcag ccggtcgcgg cgattgtcga tcacacgggt
      961 cgccatgatg cccggaatcg cgcaggcgaa actcgacaac agcggaatga aggcgcggcc
     1021 gtgcaggccg gcgcctccca tgatccggtc catcaggaag gccgcccgtg ccatgtagcc
     1081 gaaatcctcc agcagcagga tgaacaggaa gatcatgacg atctggggca ggaacacgat
     1141 tacgctgccg accccggaga tcacgccgtt ctggaggaag ctctgcaaca ggcctgcggg
     1201 cacggtatcg tgaaccaatt gcccgagcgc tgcgaaggcg ctcgacagca attccatcag
     1261 cggctgcgcc cacgcgaaca ctgcctggaa catgacgaag aggatcgccg ccaggatgac
     1321 gagccctgcc accgggtgca gcacgaccgc atcgatccgc cccgtccagg agtcgggccg
     1381 cgccggcagg ctcacggccg cggcgatgat gcgatccgcc tcgcgctggg tggcgcgcaa
     1441 ttgcgccacg gtgagcggct cccagagatt ttgccggtcc ggcgtttgcg cctgcgccgc
     1501 gatctcgtcg gtcaggcgca gcagatcgcc ggtgccgccc ttgcgcaccg cgatcgacgt
     1561 cacgacggga acgccgagcg cttcggacag ccgcgggaca tcgacgctca cgccgcggcg
     1621 ggtggcgatg tcgaacatgt tgagcaccag gatcagcggc cggccggtgc tcttgagctc
     1681 gagcaggaga cgaatcgtga gccgcagatt ggtggaatcg gccacacaaa gcac
//
LOCUS       DASWQN010000747         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2051873, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000747 DASWQN010000000
VERSION     DASWQN010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2051873"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..>1713
                     /locus_tag="VGC82_12690"
     CDS             280..>1713
                     /locus_tag="VGC82_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424170.1"
                     /translation="MNRQDGLNMVNGIDRRGFLKTAALAAAAPLAAPRLSLAADAKTL
                     RFIPQANLANLDPIWTTQYVVRNGALLIWDMLYGVDDKLQPQPQMVEGHEASADFKTW
                     TFKLRPSLKFHDGQPVLAKDVTASILRWMARDATMGGPIKKQMDALEVVDDRTFRFRL
                     NKPYSKMLFALGKSSTPSLFIMPERIAATDPFKQITEYVGSGPMRFNKNEWIPGAKAI
                     FEKFDAYAPRAEKANWLSGGKRINFDRIEWQIVPDVATAAAALQNGEVDWWETPSADL
                     VPLLRKNPNIAVDIADPLGNIGSFRINHLHPPFNDVRARRAIQIALSQEDYMGAVVGD
                     DQTLWKTIPGFFTPDTPLYTENGGEKLKGKRDYELAKKLLAESGYKNEPVVLLVATDV
                     NITKAQGDVTADLLKQIGMNVDYQALDWGTVGQRRAKKEKPSEGGWNIFHTWHAGADC
                     VNPAPYIALDSSGDTAGFGWPKSDLVQE"
BASE COUNT          370 a          511 c          526 g          306 t
ORIGIN      
        1 tactaatcaa aataatatgc catgtgatct ccatcgggat tagccaggtt cgggcttgcc
       61 agcttcggcg gcgattgtgg ccgtggtgac gctccccacg accgggtatc agttgtcgct
      121 attcgcggtt ccggcgccga caagtccttc cgatccggcg aaacacgttt tatttgcaac
      181 gaaccccgat cgccgccagc acgcggatct gaccactttc ttttcgcacc gcagtacggc
      241 acggtgcgca taggtggcct gcggcaggcg tatcgaccaa tgaacagaca ggacggcttg
      301 aacatggtca atgggatcga tcgtcgaggt ttcttgaaaa cagccgcgct cgcggcggcg
      361 gctccgcttg ccgcgcctcg gctgtccctg gcggcggatg caaaaacgtt gcgcttcatc
      421 ccacaggcga acctcgccaa tctggacccg atctggacga cgcaatatgt cgttcgcaat
      481 ggcgccctgc tgatctggga catgctctac ggcgtcgacg acaagctgca accgcagccg
      541 cagatggtcg agggtcacga agccagtgcc gacttcaaaa cctggacctt caagctgcgc
      601 cccagcctga aattccacga cgggcaaccg gtgctggcaa aggacgtgac ggccagcatc
      661 ctgcgctgga tggctcgcga cgcgacgatg ggcggcccga tcaagaaaca gatggatgcg
      721 ctggaggtgg tcgatgaccg gacattccgc ttccggctga acaagcctta ctccaagatg
      781 ttgttcgccc tgggcaagag cagcacgccc agcctgttca tcatgccgga acgcatcgcc
      841 gccaccgacc cgttcaagca gatcaccgaa tacgtcggat ccggcccgat gcggttcaac
      901 aaaaacgaat ggattcccgg cgccaaggca atcttcgaga agttcgatgc gtatgccccg
      961 cgggcggaaa aggccaattg gctgtccggg ggcaagcgta tcaacttcga ccggatcgaa
     1021 tggcagatcg ttccggatgt cgcgacggcc gccgcggcgc tgcagaatgg cgaagtcgat
     1081 tggtgggaga cgccgtcggc cgacctggtg ccgttattaa ggaagaatcc caacatcgcg
     1141 gtggatatcg ccgacccgct gggcaatatc ggatcttttc gcatcaacca cctgcacccg
     1201 ccgttcaacg atgtccgtgc ccgccgcgcc atccagatcg ctctgagcca ggaagactac
     1261 atgggtgccg tggtcggtga cgaccaaacc ttgtggaaga caataccggg attcttcacg
     1321 cccgacacgc cgctctacac ggaaaacggc ggggaaaagc tgaagggcaa acgcgattac
     1381 gaactggcga agaagctgct tgccgagtcc ggctacaaga acgaaccggt ggtgctgctg
     1441 gtggcgaccg acgtcaatat caccaaggcg cagggcgatg tgacggcgga tctgctgaag
     1501 cagatcggta tgaacgtgga ttaccaggcg ctcgattggg gcacggtcgg acagcggcgc
     1561 gccaagaagg aaaaaccgtc cgagggtggc tggaacattt ttcacacctg gcacgccggc
     1621 gccgactgcg tgaatccggc gccgtacatc gctttggact caagcgggga cactgccggg
     1681 ttcggctggc cgaagtcgga tctggtgcag gag
//
LOCUS       DASWQN010000748         3003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2315216, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000748 DASWQN010000000
VERSION     DASWQN010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2315216"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1070)
                     /gene="phnE"
                     /locus_tag="VGC82_12695"
     CDS             complement(144..1070)
                     /gene="phnE"
                     /locus_tag="VGC82_12695"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEY0424171.1"
                     /translation="MTALGPTDDPAYRLFQQRFGALRRSRQTESLLTLAAVILLFVIA
                     AAWTDFSPQKVVNGIPRIGEYFGKLFSIEPERNAAPIGVLALAHVFGGVKEPQSIAYW
                     FYRIDTYLSLLWQTVEMAILATAIGFGAAFALSFPATSRFVGSRFIVMLTRRLLEIMR
                     TVPQIVLAFILVWPFGIGPLAGIVAIAIHTTGTLGKLFCELNENAELGAVDGIRSVGG
                     SWLAQIRYGVVPQVMPGFLSYALLRFEINVRSSTIIGFVGAGGIGQELKRVIGFNIYE
                     EVSAIVILILLIVVAIDLTSERIRHRFIGALV"
     gene            complement(1102..2061)
                     /gene="phnD"
                     /locus_tag="VGC82_12700"
     CDS             complement(1102..2061)
                     /gene="phnD"
                     /locus_tag="VGC82_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0424172.1"
                     /translation="MIRQTVRRLAAAGLALLIPLAADAQDSKQQDSKQQDWKQQVKEL
                     RVGLLGGENTSSRLARYDGFQHLLQEKLGIPVKLYPAADYAGVMQAMGAGQVDLSEFS
                     PSAFAGAWLDCKCVEPTVVPQEKDGTIFYISVMVARKDSGITSLEQMKGHSLAWADPN
                     SASGYLIPSASLKAGGIDLADGAYFSRTGFAGGHEQGVVAVLNHQYDASVTWTSGQGD
                     IAQGFSRGALRGMVDRGMLKMSDVNIIWKSGKIPNGPWGLRSALPAGLKSAFTDFMLD
                     LPKSHKDIYDAVEQGSGVGYQLATLGLYKDIIELRETERRGSR"
     gene            complement(2085..2891)
                     /gene="phnC"
                     /locus_tag="VGC82_12705"
     CDS             complement(2085..2891)
                     /gene="phnC"
                     /locus_tag="VGC82_12705"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEY0424173.1"
                     /translation="MLQLVDLTRRFGDKKAVNQAGVTIGDGEMVGIIGRSGAGKSTLL
                     RLINRLIDPSEGQILYHGTDVTALRGSALRAWRARTAMIFQQFNLVNRLDVLTNVMCG
                     RLYDMPAPRVLLKWFTPAERALAVQALDRLGIAELAMNRADTLSGGQQQRVAIARALM
                     QRPRILLADEPIASLDPLNAKLVMDALADINRRDGITILTNLHTLDTARSYCRRIIGM
                     STGRIVYDGAPDGLTDHVLREIYGQAGQEALDERLTSISLPNAALAAFAG"
BASE COUNT          570 a          925 c          965 g          543 t
ORIGIN      
        1 gaacagccac atcaggtaga acaggtaagc gaagacgccc gccagcagca gccagcgcag
       61 ccagcctttg ccacgaaaca cctcgggatg acgggcgcgt tcagcggcga ggtccagaac
      121 cgtgccgctc atacccgtgc cgctcatact agggcgccga tgaagcggtg acggatacgc
      181 tccgatgtca ggtcgatggc gacgacgatg agcaggatca ggatgacgat ggcgctgact
      241 tcttcgtaga tgttgaagcc gatcacccgt ttcagctcct ggccgatgcc gccggcgcca
      301 acaaagccga tgatggtgga cgaccgcacg ttgatctcga accgcaacag cgcgtagctg
      361 agaaagcccg gcatgacctg cggcacgacg ccatagcgga tttgcgccag ccaggaaccg
      421 ccgaccgacc ggatgccgtc gaccgcaccc aactcggcgt tttcattcag ctcgcagaac
      481 agcttcccga gcgtgcctgt cgtgtggatg gcgatcgcga cgattccggc gagcgggccg
      541 atgccgaacg gccagaccag gatgaaggcg aggacgattt gtggcacggt gcgcatgatc
      601 tcgagcaacc gccgcgtcag catgacgata aagcggctgc cgacaaagcg gcttgtggcg
      661 gggaatgaca gcgcaaaggc ggcgccgaaa ccgatcgcgg tggccaggat ggccatctcg
      721 acggtctgcc acaacaacga caggtaggtg tcgatccggt agaaccagta ggcgatggac
      781 tgcggttcct tcactccgcc aaacacatgc gccagcgcca gtacgccgat cggggcggca
      841 ttccgctcgg gctcgatgga aaacagtttg ccgaaatatt cgccgatccg agggattccg
      901 ttgacgacct tctgcggcga gaaatcggtc caggcggcgg cgatgacgaa cagcaggatg
      961 acggctgcaa gagtcagcag cgactcggtc tggcgcgacc gccgaagtgc cccgaagcgc
     1021 tgctggaaca gccggtacgc gggatcatcg gttggaccga gagcggtcac cgttggcgcc
     1081 ttcgttcggc aggcggagtc attaccggct gccccgccgc tcggtttccc gcagctcgat
     1141 gatgtccttg tacagaccca gggttgccag ttgatagccg acacccgatc cctgctctac
     1201 tgcgtcgtag atgtccttgt gcgactttgg caggtcgagc atgaagtcgg tgaacgccga
     1261 tttcaggcca gccggcaacg ccgaacgcaa gccccacggg ccgttgggga tcttgcccga
     1321 tttccagatg atgttgacgt cggacatctt cagcatgccg cggtcaacca tgccgcgcag
     1381 cgcgccccgg ctgaagcctt gtgcgatgtc gccctggccg gaggtccatg tgacgcttgc
     1441 gtcgtactgg tggttcagga cggcgacgac gccctgttca tgtccgccgg cgaatccggt
     1501 gcgcgagaaa tacgcgccgt ccgccaggtc gatgccgccg gccttcagcg acgcgctggg
     1561 gatcaggtag ccggaggcgg aattcggatc ggcccaagcc agtgaatggc ccttcatctg
     1621 ctccagtgac gtaatgccgg aatccttcct ggcgaccatc acggagatat agaagatcgt
     1681 gccgtccttt tcctgcggca cgaccgtggg ctccacgcat ttgcagtcca gccacgcacc
     1741 agcgaaggca gagggactga attccgacag gtccacctgg ccagcgccca tcgcttgcat
     1801 cacaccggca taatcggccg cgggatagag tttcaccgga atgccgagct tttcctgcag
     1861 caggtgttga aagccgtcgt accgggcaag gcggctggag gtgttctcac cgcccaacag
     1921 accgacacgc aattccttga cctgctgctt ccagtcctgc tgcttcgagt cttgttgctt
     1981 cgagtcttgg gcgtccgctg ccaacgggat caacagcgca agcccggcag cggccagacg
     2041 tctgacggtt tggcggatca taaagtcttc tcccggtgga agccctaccc cgcgaaggcg
     2101 gccagggctg cgttcggaag actaatggag gtcaggcgtt cgtccagggc ttcctggccg
     2161 gcttgtccat agatttcgcg cagcacatgg tcggtcagcc catcgggtgc gccgtcgtaa
     2221 acaattcgtc cggtggacat gccgatgatg cggcggcaat agctgcgcgc ggtgtccagc
     2281 gtgtgcaggt tggtcaggat ggtgatgccg tcccggcgat tgatgtcggc cagcgcatcc
     2341 atcaccagct tggcgttcaa cggatcgagc gaggcgatcg gttcgtcggc cagcaggatg
     2401 cggggacgct gcatcagcgc cctggcgatg gcgacccgtt gctgctgccc gcctgacaag
     2461 gtgtcggcac ggttcatggc caactcggcg atgcccaacc ggtcgagcgc ctggaccgca
     2521 agcgcccgtt cggccggcgt aaaccacttc agcaatacgc ggggtgccgg catgtcatac
     2581 agccggccgc acatgacgtt ggtcagcacg tccagccggt tcaccaggtt gaactgctga
     2641 aagatcatcg cggttcgggc ccgccaagcc cgcaacgcgc tgccgcgcag cgcggtcacg
     2701 tcggtgccgt ggtaaaggat ttggccctcg gacgggtcga tcagtcggtt gatcaggcgc
     2761 aacaaggttg attttccggc acccgagcgg ccgatgattc ccaccatctc gccgtcgccg
     2821 atggtcacgc ccgcttggtt taccgccttc ttgtcaccga accggcgggt gagatcaacg
     2881 agttgcagca atggttcgcc ttactggtgt tggtgcggtc gagcgcggag gcgccgggct
     2941 tttggcatct agcagcggag catggccggt agtgtgacgg tttcatgatc ctgacgccga
     3001 att
//
LOCUS       DASWQN010000749         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2447245, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000749 DASWQN010000000
VERSION     DASWQN010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2447245"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..950)
                     /locus_tag="VGC82_12710"
     CDS             complement(243..950)
                     /locus_tag="VGC82_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0424174.1"
                     /translation="MPGVLDGAIALVTGASRGIGAAVSVELARLGARVVITARTQGGL
                     EETDDAIRAIGGEATILPLDLREGEQIDAIGPTLFQRFGRLDVLLHNAGALGRLTPVS
                     HILPDDWADVVAVNLSASWRLIRTCDPLLRRAPAGRAVFVTEQRAREPRAYWGAMGAT
                     KAGMEHLALTWAQEVATTFVRVNLFDPGPVATRMRSNAFPGEKPATLPTAERVAPALA
                     ALCLPLEQRNGSLVHFQ"
     gene            complement(950..2416)
                     /gene="purF"
                     /locus_tag="VGC82_12715"
     CDS             complement(950..2416)
                     /gene="purF"
                     /locus_tag="VGC82_12715"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY0424175.1"
                     /translation="MAFNSRDIDDDSLHEECGVFGVWNTPDAAAVTALGLHALQHRGQ
                     EATGITSFDGARFHSHRGMGLVGDNFGDARVIEKLPGRIAIGHNRYATTGDTVLRNVQ
                     PLYADFEFGGFAVAHNGNLTNAHILHRSLVRRGCLFQSTTDSEVIVHLIAISLYSTVV
                     DRLIDALKQVVGAYSLVALSNEALMGVRDPLGVRPLILGRIGSEGTGGWVLASETCAL
                     EIVGADFIRDVEPGEIVVINDKGVHSVKPFGRQQERFCVFEYIYFARPDSVMEGTPVY
                     DARKAIGAELAREADVPADVIVPVPDSGVPAAMGYSKQSGIPFELGIIRNHYVGRTFI
                     EPTEHIRHLGVKLKHSANRPVLKGKRVVLVDDSIVRGTTSKKIVEMVRAAGAKEVHMR
                     ISSPPTTNSCFYGIDTPERSKLLAANHTVEEMAALIGADSLAFISIDGLYRALGKDGR
                     DPKKPYYCDACFTGDYPIPLPDQQDNSDRQLSLLAAHH"
     gene            complement(2428..>2647)
                     /locus_tag="VGC82_12720"
     CDS             complement(2428..>2647)
                     /locus_tag="VGC82_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424176.1"
                     /translation="MVFPIERWPDVVLNARTLTPTYEAARWVRSQLPEPYRPHTLDPP
                     PAGRRPTAEALLHASPQGRATGKHIERE"
BASE COUNT          461 a          879 c          855 g          452 t
ORIGIN      
        1 tgaaccagca gaacttcacc aagacggcga attgctgggg ggtcggcaac aaccgcggcg
       61 gcaccaacac caaccggatc gtcatggcgg tccggcggta gtcttgcatc ccatcacttg
      121 tcgcacccct gccgctgacc aggccggcgg cactacgagt tgtttgtgat acggggtttt
      181 cggacgggtg gatcggaggg gcatgccgaa ccgagcgcca tcccacccaa gaacgcgcca
      241 gcctattgga aatgtactaa cgagccgttg cgctgttcca gcggcaggca aagcgctgcc
      301 aacgccggcg ccacccgttc cgcagtcggc aacgtcgccg gcttctcgcc gggaaaggca
      361 ttggatcgca ttcgggtcgc cactggcccc ggatcgaaca ggttgacccg aacgaatgtg
      421 gtcgcgactt cctgcgccca ggtcagcgcc aggtgctcca tccccgcctt cgtcgcaccc
      481 attgcccccc agtatgcccg aggctcccgc gcccgctgtt ccgtcacgaa caccgcccgt
      541 ccggccggcg cccgacgcag caggggatcg catgttcgga tcagccgcca gctcgcggac
      601 aggttcacag ccaccacgtc cgcccaatcg tccggcagta tatgggacac cggcgtcagt
      661 cgccccagcg ccccggcgtt gtgcaacagc acgtccagcc ggccgaagcg ctggaacaag
      721 gtcgggccga tcgcgtcgat ctgctcccct tcgcgcaaat ccagcggcag gatcgtggcc
      781 tcgccgccga tcgctcggat cgcgtcgtcg gtttcctcca ggccaccctg ggtgcgcgcg
      841 gtgatcacca cgcgcgcgcc gaggcgggcc aactcgaccg aaacggccgc gccaatgcct
      901 cggctggcac ccgtcaccag ggcgatggcg ccatccagca cgcccggcat cagtgatggg
      961 ctgccagcag gctgagctgc ctgtccgagt tatcctgttg gtcgggcagc ggaatcggat
     1021 agtcgccggt aaagcaggcg tcgcaatagt aaggtttctt cggatcgcgg ccatccttgc
     1081 caagcgctcg gtacagcccg tcgatcgaaa taaacgccaa actatcggcg ccgatcagtg
     1141 ccgccatttc ctcgaccgtg tggttggccg ccagcagctt gctgcgctcc ggcgtgtcga
     1201 tcccgtagaa gcaggagttc gtcgtgggcg gcgacgaaat gcgcatgtgc acttccttgg
     1261 cgccggccgc gcgaaccatc tcgacgatct tcttgctggt ggtgccgcgc acgatcgaat
     1321 cgtccaccag caccacgcgc ttgcccttca gcaccgggcg gttggccgag tgcttcagct
     1381 tcacgcccag atggcggata tgttcggtgg gctcgatgaa ggtgcggccg acataatggt
     1441 tgcggatgat cccaagctcg aacgggatgc cgctttgctt cgaatagccc attgccgcgg
     1501 gaacgccgga atccggcacc ggcacgatga cgtcggccgg cacatcggcc tcacgcgcca
     1561 gttcggcgcc gatcgccttg cgcgcgtcat agaccggcgt gccctccatc accgagtcgg
     1621 gccgagcgaa atagatgtat tcaaacacgc agaaccgctc ctgttggcgg ccgaatggct
     1681 tgacgctgtg tacgcccttg tcgttgatga cgacgatttc cccgggctcc acatcacgga
     1741 tgaagtcggc gccgacgatc tccagggcgc aggtctcgct ggccaggacc cagccaccgg
     1801 tgccctcgga tccgatgcgg cccaggatca gcggccgaac ccccaacgga tcgcgcaccc
     1861 ccatcaaggc ttcgttggac agcgcgacca gggaataggc gccgaccacc tgcttcaagg
     1921 cgtcgatcag ccggtccacc acggtggaat acaggctgat ggcgatcagg tggacgatga
     1981 cctcggagtc ggtggtggac tggaacaggc agccgcgacg caccaaggag cggtgcagga
     2041 tgtgggcatt ggtcaggttg ccgttgtgcg ccaccgcgaa gccgccgaat tcgaaatctg
     2101 catacagcgg ctgcacgttg cgcagcacgg tgtcgccggt ggtggcatac cggttgtggc
     2161 cgatggcgat gcggccgggc agcttctcga taacccgggc gtcgccgaaa ttgtcgccga
     2221 ccaggcccat gccgcggtgc gagtggaagc gggcgccgtc gaagctggtg atgccggtgg
     2281 cttcctggcc gcgatgctgc agggcgtgca ggcccagcgc cgtcaccgcc gcggcgtcgg
     2341 gcgtgttcca gacgccaaac acgccgcact cctcatgcag ggagtcgtca tcaatatccc
     2401 ggctgttgaa tgccatcgtg acagttccta ttcgcgttcg atgtgcttgc cggtcgcgcg
     2461 gccttgcgga ctggcatgca gcagggcttc agcagtgggt cgtcggcccg ctggcggcgg
     2521 gtcgagcgtg tggggacggt aaggttcggg caattgagaa cgcacccacc gcgcggcctc
     2581 ataggtcggc gtcagtgtcc gagcgttgag tacgacgtcg ggccaacgtt cgatcgggaa
     2641 caccatc
//
LOCUS       DASWQN010000750         3706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_468081, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000750 DASWQN010000000
VERSION     DASWQN010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468081"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1029
                     /gene="speE"
                     /locus_tag="VGC82_12725"
     CDS             163..1029
                     /gene="speE"
                     /locus_tag="VGC82_12725"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEY0424177.1"
                     /translation="MPTDSWINETLYPDWGQRFRVTRELARVKSPFQDIAIFESSSHG
                     RVMVLDGVIQITEGDEFVYQEMLAHVPLLAHGAAANVLIIGAGDGGVLRRVLQHQSVK
                     RAVMVEIDGEVIRLSKEFLPRIAGDAWTDKRADVIVGDGIDYVKRAPDGSFDAIIVDS
                     TDPIGVGEVLFTDEFYANCARILTDRGLVVNQCGVPFMQADELHETSVRRRKFFSHVT
                     AYVAAVPTYVGGFMTLGWAAKDASLALASPETIRARAARAGILGTTNYWTPEIHTGAF
                     NLPPYIARHLPD"
     gene            1389..2270
                     /locus_tag="VGC82_12730"
     CDS             1389..2270
                     /locus_tag="VGC82_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0424178.1"
                     /translation="MRFAVASVVVASFLLTAPVVPAQAQVDSREGIALQNQIYQLRQE
                     LRAVQDQAARGGGSAIRPPAYAPPPQQQYGGNELVTQLLTRVDSLEDQVRQLRGRIDE
                     TQNQLQRQNDNLGKRIDDLAFQVNPQGGQPGFAPPGANPPASTLRAPASVPPGPPPRP
                     ASPPGPRTPEVALQDGNAALARRDYPAAEAAAREVLANRTSPRAYDAQFLLAQALSGE
                     RQYPQAAVAYDDVYNRSRTGSHAQDARLGLASSLTAINEKKAACDVLSRMRTEFPQLR
                     SDISAGMSATSQKAGCR"
     gene            2261..3514
                     /gene="tilS"
                     /locus_tag="VGC82_12735"
     CDS             2261..3514
                     /gene="tilS"
                     /locus_tag="VGC82_12735"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY0424179.1"
                     /translation="MPLTIMLKAMFAAAMARLGPFEPNPALAVAVSGGADSMALALLA
                     ADWVRQRDGATQAFVVDHGLRPASGVEAEVTIQRLARFGIPAKLLALTTLKPGSALAE
                     RARIMRYKVLSDACAQAGILHLLLGHHAADQVETLAMRVLRDSHTHGLAGMSALRETT
                     VLRLLRPLLAIEPGLLRRFLMEYETAWVEDPSNQDVRAMRARLRQGFANPSGKGNDLL
                     HALTSVGRRRCQEEAATADELARRVTIRPEGFALLSPNRIGVAALRSLIGTIGGARYL
                     PGLGQTTELAAQPKPATLAGVRILPAGRFGDGLLIVREEAGIGDPVPALPDGIWDNRF
                     RLIAPWDIPAGVMIGKLGSDAERFRARTDLPSAVLRTLPAVRMGKVLAAVPNLGYINP
                     ENDVRVTMLFSPRRPLAGSGFVPTS"
BASE COUNT          649 a         1180 c         1189 g          688 t
ORIGIN      
        1 ccgcacgaca gcgttcccgc attgaaagca gcgttccgac cgcggacgat caaccttggc
       61 gagcagcggc gcggtctgat cggctgatcg atcccggtct aacccgaaca tcagtgacgg
      121 cccgagcgga tgcttgggcc gtcaacgtat tggagccagg cgatgccgac cgatagctgg
      181 atcaacgaga cgctgtatcc ggactggggc cagcgttttc gggtgacgcg cgaactggcc
      241 cgggtgaaga gccccttcca ggacatcgcg atcttcgaaa gttcgtccca tggccgcgtc
      301 atggtactgg acggcgtcat ccagatcacc gagggcgacg aattcgttta tcaggagatg
      361 ctggcccatg tgcccttgct ggcccatggc gccgcggcaa acgtattgat catcggcgcc
      421 ggcgacggcg gcgtgctgcg ccgcgtcctg cagcaccaaa gcgtgaagcg cgccgtgatg
      481 gtcgaaattg acggcgaggt catacgcctg tcgaaggaat tccttccacg gatcgccggc
      541 gatgcctgga ccgacaaacg ggcggatgtc atcgtcggcg atggcatcga ttacgttaag
      601 cgggcgccgg acggctcctt cgatgccatc atcgtcgatt cgaccgatcc gatcggcgtg
      661 ggtgaagtgc tgttcaccga cgagttctat gccaattgcg cccgcatcct gacagatcgt
      721 ggactggtcg ttaatcaatg cggcgttccg ttcatgcagg cggatgaact gcatgagacc
      781 agcgttcggc gccgcaaatt tttttcgcac gtcaccgcct atgttgccgc ggttcctacc
      841 tatgtcggcg ggtttatgac cttgggttgg gccgcgaagg acgcctccct cgcgttggca
      901 tcgccggaaa cgatccgcgc ccgtgcggcg cgggcgggaa ttttgggcac gacgaactac
      961 tggacgccgg aaatccatac tggggcgttc aacctgcccc cctatatcgc gcgccatctg
     1021 cctgactagg gcgtcgcgcc gaaacgccca acaccggctc aggccccaca ttggttcagg
     1081 ccccacattg gttcaggcag cgtcggtccg cacccgatca cgcagcgccg gccgcggcaa
     1141 acatcgacac gatgtcatgc cgaacttgca ttggattgcc gtgttcgcct gcgacacgat
     1201 aaaccacttt tggcctgaat gtgcaggccg caaatggtct gtctcccggt ttgaccgcct
     1261 cccggtttga ccgcccccct gtttcgccgc atgccgtcac ccgcttcgga cattccgccc
     1321 agcataatca cgctctagac tgacgcccgc cagactgatg ccggtcaata actcccgagg
     1381 aagtttgcat gcgattcgcc gttgcttctg ttgtcgtggc aagctttctg ctgacagccc
     1441 ccgtggtgcc cgcacaggcc caggtggaca gccgggaagg catcgcgttg cagaaccaga
     1501 tctatcagct ccgccaggag ctgagggcgg tccaggacca ggcggcgcgc ggcggagggt
     1561 ccgccatccg tccaccggcc tacgcgccgc cgccacaaca gcaatatggc ggcaacgaac
     1621 tcgtcaccca gcttttgacg cgcgtcgact cgctggagga tcaggtccgc caactccggg
     1681 gccggatcga cgagacgcag aaccagcttc agcggcaaaa cgacaatctg ggcaagcgga
     1741 tcgacgacct ggcatttcag gtcaatcccc agggtggtca gcctggattt gcaccgcctg
     1801 gcgccaaccc gccggcttcc acgctacgcg cgcccgcctc tgttccaccg ggtccgccgc
     1861 ctcgccccgc ctcgccgccg ggtccgcgga cgcccgaggt tgccttgcag gacggcaacg
     1921 ccgcgcttgc ccgtcgtgac tatcccgccg cggaggccgc cgcgcgggaa gtgctggcga
     1981 accgcacctc gccgcgagcg tacgatgcgc agttcctgtt ggctcaggca ctgtcgggcg
     2041 agcggcaata tccccaggcg gccgtcgctt acgacgatgt ctataatcgg tcgcgcaccg
     2101 gttcgcatgc ccaggatgcc cgccttggcc tggcatcgtc gctgaccgcg atcaatgaga
     2161 aaaaggcggc ctgcgatgta ttgagccgga tgcggacgga gttcccgcag ctccgctccg
     2221 acatcagcgc cggcatgtcg gcaacgtcac agaaggcggg ttgccgctaa ccatcatgct
     2281 gaaggccatg ttcgcggcgg caatggccag gctggggccg ttcgagccta atcccgcgct
     2341 ggcggtcgcg gtatccggtg gcgcggacag catggcgctc gcccttctcg ccgcggattg
     2401 ggtgcgccag cgcgacgggg cgactcaggc gttcgtcgtc gatcacgggt tgcggccggc
     2461 ctccggcgtc gaagcggaag tcaccatcca acgcctcgcg aggttcggca ttccagcgaa
     2521 gctgctggcg ctgacgacct tgaaaccagg gtccgcgctg gccgaacggg cacggatcat
     2581 gcgttacaaa gtgctttccg atgcatgcgc gcaagccggc attctccatc ttttgctcgg
     2641 tcaccatgcc gcggatcagg tcgagacgct ggcgatgcgg gtgctgcgcg acagccacac
     2701 gcatggcctt gccggtatgt ccgccctgcg cgagaccacc gtcctacggc tgctgcgtcc
     2761 gctgctggcg atcgagcccg ggctgcttcg gcggttcctg atggaatatg aaacggcgtg
     2821 ggtcgaagac ccgtccaacc aggacgtgcg ggcgatgcgc gctcggttgc ggcagggttt
     2881 cgccaaccca tccgggaagg gaaacgacct tctccatgcc ctgacatccg tcggcaggag
     2941 gcggtgccag gaggaagctg cgacggctga tgaactggcg agaagggtca cgatccgccc
     3001 tgaaggattc gcgctgctgt cgccgaaccg cattggcgtg gcggcgctgc ggtcccttat
     3061 cggaacgatc ggtggagcgc gctatctccc tgggctggga cagacaacgg aactggcggc
     3121 acaaccaaag ccggcaacgc ttgcgggcgt gcgcatcctg ccggcggggc ggtttggcga
     3181 cggcctactg atcgtgcgtg aggaagccgg gatcggcgat ccggttccgg cattgcccga
     3241 cggtatctgg gacaaccggt ttcggttgat cgcgccgtgg gatattcccg caggtgtgat
     3301 gatcggcaaa ctcggttcgg atgcggaacg gtttagagcg cgcacggacc tgccctccgc
     3361 cgttctgcga acgctgcctg cggtcagaat gggtaaagtt ctggctgcgg tgccaaatct
     3421 aggatatatc aacccggaaa atgacgtgcg ggtgacgatg ctgttcagcc cgcggcgacc
     3481 gctcgcaggt tcgggatttg tccccacctc ctagtccggc ggcgtgaaga cgactgcccg
     3541 cttgttattg gccccggttt ggttaacgat cgtcatgcgg cggggatgta gggatggacc
     3601 aagcgcacta tgttagctgt cttgctgggt cggttatcgt ccaagatcgc ctggcgtcca
     3661 attcggactg agaacgtgag caaagtcata tgagcaattt tggccg
//
LOCUS       DASWQN010000751         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1788360, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000751 DASWQN010000000
VERSION     DASWQN010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788360"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VGC82_12740"
     CDS             complement(<1..739)
                     /locus_tag="VGC82_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY0424180.1"
                     /translation="MSRPISRRLLLGTTAATLPLPGLISARGDAPEFEMKLGNDNPMS
                     HPNTIRQQQAADRILMETNGRVKISVFPNNQLGGDTDMLSQLRSGALEFMSLSGLILS
                     TFTPLSSIYGIGYAFPDYKKVWAAVDGDLGAMIRASVDKSGLYAFEKIWDNGFRQISS
                     STHPIRTPDDLKGYKIRVPVSPLWTSMFEAFGAAPASINFSEVYSALQTKIVEGQENP
                     LVNIETAKLYEVQKYVSMTRHMWDGFIM"
     gene            complement(845..2335)
                     /locus_tag="VGC82_12745"
     CDS             complement(845..2335)
                     /locus_tag="VGC82_12745"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY0424181.1"
                     /translation="MRGHLFGMSETNIFQQPAGPQAASQAVAANPNVAGETADQALVR
                     AQAAAQAKRMNEASGICADVLAASPDHPAALALLGIIAAMVGDAEKGIVLMRRAIQLR
                     PGNATWYGHLSSMCRATYRLEEALTAGQESIRLDSNSPEHLVNLSLIFVDADDREHAV
                     ACLLRAIGLKHDHADGHLALAQNLLAMGDFDAGWLEYEWRNLTEAGKASMPSMTSAPW
                     NGMRIPTGRLLLVGDQGYGDTIQFARYISMAAERCQEVILGCSQEMGPLLSKMPGVSQ
                     YCHQWTDVPPHAAHCRLSSLPYLFRTQLDTIPGQAPYLKADPARIAHWKNRLDATLPA
                     GRKRVGLAWSGRPTHPNDRRRSLPLTQFASLTQAGPAAFVSLQKPMPERDLETIPKFP
                     GMVNLADELTDFGETAALMENLDLVITVDTAVGHLAGALGKAAWILIPKAADWRWMVD
                     REDSPWYKSVRLFRQPKPGNWDDPIGRLQIALTRHLAGSLAESAAG"
BASE COUNT          452 a          797 c          800 g          505 t
ORIGIN      
        1 gcataatgaa cccgtcccac atgtgccggg tcatcgagac gtatttctgc acctcatata
       61 atttggccgt ctcgatgttc acaagcgggt tctcctggcc ttcgacgatc ttcgtctgca
      121 gggccgaata gacctcgctg aaatttatgc tcgccggggc ggctccgaag gcttcgaaca
      181 tgctggtcca tagcgggctg accggcacgc gaattttgta gcccttcagg tcgtcgggtg
      241 tccgaatggg atgggtactg ctgctgatct ggcggaagcc gttgtcccaa atcttctcga
      301 acgcatacaa tccggacttg tccaccgagg ccctgatcat cgcgcccagg tcgccatcga
      361 ccgctgccca gactttcttg tagtctggga aggcatagcc gattccgtat atcgagctca
      421 gcggcgtgaa cgtggaaagg atcagccccg acaggctcat gaattccagg gcgccggatc
      481 gaagctgcga cagcatgtcg gtgtcgccgc ccagttggtt gttgggaaag accgagattt
      541 tcacccgccc gttggtttcc atcaggatcc ggtccgcggc ctgttgctgg cggatggtgt
      601 tcggatgcga catgggattg tcgtttccca gcttcatctc gaactcgggc gcgtcgccgc
      661 gggcggaaat taggcccggc agcggcaagg tagcggcggt cgtacccagc aagagccggc
      721 gggaaatcgg gcgggacatc gtgttcctcc attgccgggt cgtgtcgtgc ccgcgccgga
      781 ggaacctgtc ggtggccacc ccggtggtca agacccgagg ctgtgcagaa ctttccgatg
      841 gggcctaacc ggcggcgctt tccgccagcg accccgcgag gtgacgggtc agggctattt
      901 gcaaccgtcc gatcggatcg tcccagttgc cgggcttcgg ttggcggaac aaccggacgg
      961 atttgtacca tgggctgtcc tcgcgatcca ccatccaccg ccagtcggcg gctttcggaa
     1021 tcaggatcca ggcggctttg ccaagcgcgc cggccagatg tcccaccgcg gtatccacgg
     1081 tgatgaccag gtcaagattc tccattaggg ccgcggtctc gccaaaatca gtcaactcgt
     1141 cagccaggtt gaccatgccg ggaaatttcg gaatggtctc caggtcccgc tccggcatcg
     1201 gcttctgtag cgagacgaag gcggccggac ctgcctgcgt caacgaggcg aactgtgtca
     1261 gaggcagcga gcggcggcgg tcgttcggat gcgtcggacg tcccgaccaa gccaatccga
     1321 cccgtttcct gccggccggc agtgtggcgt cgaggcggtt cttccaatgc gcgatgcggg
     1381 ccggatcggc cttcagatag ggcgcttgtc cgggaatcgt atcaagctgc gtgcggaaca
     1441 gatatggcag gctggacagg cggcaatgcg cggcgtgggg cggcacatcg gtccattgat
     1501 ggcaatactg cgaaaccccc ggcatcttcg acagcaacgg ccccatctcc tggctacagc
     1561 caaggatcac ctcctggcag cgttcggcag ccatcgagat ataccgagcg aactggatgg
     1621 tatcgccgta accttgatcg ccgaccagaa gcaggcggcc ggtcggaatt ctcatgccgt
     1681 tccacggtgc cgaggtcatc gacggcatgc tcgcctttcc cgcctcggtc aggttgcgcc
     1741 actcgtattc gagccagccg gcgtcgaagt cccccatcgc cagcagattc tgcgccagcg
     1801 ccagatgacc gtcggcatgg tcgtgcttca agccaatcgc gcgcagcagg caggcaacgg
     1861 cgtgttcccg atcgtccgca tcaacgaaga tcagcgacag attgaccaga tgctccgggc
     1921 tgttggagtc caggcggatc gattcctgtc cggctgtcaa agcctcctcc aaccggtagg
     1981 tggcgcggca catcgaactt agatgtccat accaggtcgc attgcctggc cggagctgga
     2041 tagcccggcg catcagtacg atgcccttct ccgcgtcacc gaccatggcg gcgatgatgc
     2101 ccaacagcgc gagcgcggcc ggatggtccg gcgaggccgc gagaacatcg gcgcaaatgc
     2161 cgctggcctc gttcatccgt ttagcctggg cggcggcctg cgcgcggacc agcgcctgat
     2221 cggccgtttc gccggcgacg ttcgggtttg cagcgacggc ttgactggcc gcctgcggac
     2281 cagccggttg ctgaaagata ttggtttcgc tcatgccgaa taaatgcccg cgcaccgggt
     2341 gcttcggcaa gtgctttcac caggctgcga cctggcccgt atgcggcttg tcggtcccga
     2401 cctatcacgg aaataggatc ggtctggcca agctggtatc gtcgtcggca agagcggtcg
     2461 tcgaccgccc cccgcaccca gggccccgaa cctgatgttc gctatgataa gcgcacccgg
     2521 tttcaaaccc tggctttttg tttcggtgtc tgcc
//
LOCUS       DASWQN010000752         1931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1127618, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000752 DASWQN010000000
VERSION     DASWQN010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127618"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VGC82_12750"
     CDS             <1..840
                     /locus_tag="VGC82_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY0424182.1"
                     /translation="RQDRLGAERRDHRKITVVDGRIGFAGGVNLDKVYENPVSAGVPE
                     DGDTKRAFWRDTAVRIEGPAVAELQKLFFGTWREQKAPPVQEADYFPPLPRQGVQTIR
                     VIGSSPGNKEPLYYASALTAMKHARQRVWLCTGYFVPPHQEREELMKIARAGLDVRIV
                     VPAFSDVQSAVYAARAAYGDLLQAGAHIHEVRDAVVHAKMAVVDDTWAVVGSSNLDRR
                     SVVFNNEVDVVILGHDSAMQVEAVMRQYMAASPEVDLAKWEQRSAAERMNELQARVWQ
                     YWM"
     gene            complement(1118..>1931)
                     /locus_tag="VGC82_12755"
     CDS             complement(1118..>1931)
                     /locus_tag="VGC82_12755"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0424183.1"
                     /translation="AEAGPEAENWTYDTMLKAAEACKKANMTFGIGLSTTPDSVDTAG
                     AMFAAFGAEVISAKGDITVRSDNVRQVLEYAQQLVKHLPADAVSYDDASNNRALISGK
                     SALIWNPPSAWAVARRDAPKVAEDCWTFPAPKGPKGRFIPMGAFSWGIWKFSSNKTAA
                     KELIEFLSQRENVEARCNASLGYDVPPFASMTDFKVWEEVGPPKGTVYHYPIRKSHHQ
                     ESWIALAPAPPEIAVQAYNRGTLPTMLAKLQSGQSIKQVQDWAQDELEGFVR"
BASE COUNT          353 a          595 c          629 g          354 t
ORIGIN      
        1 cgtcaggatc ggctgggcgc cgaacgccgc gaccatcgca agataaccgt cgtcgatggc
       61 cgcatcggtt tcgccggcgg agtcaacctg gacaaggttt atgagaaccc ggtgagtgcc
      121 ggcgtcccgg aagacggcga caccaaacgg gcattctggc gcgacaccgc ggttcggatc
      181 gaaggaccgg cggtcgcgga actgcaaaaa ttgttcttcg gcacatggcg ggagcagaag
      241 gccccgccag tccaggaagc ggactatttt ccaccgctgc cgcgccaggg ggtgcagacg
      301 atccgcgtca tcggcagttc gccgggcaac aaggagccgc tgtattacgc ctccgcgctc
      361 acggcgatga agcacgccag gcagcgtgtc tggctatgca ccggctactt cgtgcccccg
      421 caccaggagc gtgaggaact catgaaaatc gcccgggcgg ggctggatgt gcggattgtg
      481 gttccggcat tcagcgatgt gcaaagtgcg gtttatgcgg cccgagcggc atatggcgac
      541 ttgctgcagg ccggcgccca catccacgaa gtgcgggacg ccgtggtgca cgccaagatg
      601 gcggtggtgg acgatacctg ggcggtcgtg gggtcgtcga acctcgatcg tcgcagtgtg
      661 gtgttcaata acgaggtcga tgtggtgatc ctgggccacg actcggcgat gcaggtggaa
      721 gcggtcatgc ggcagtacat ggctgcttcg cccgaagtcg atctggcgaa gtgggaacaa
      781 cgctcggccg ccgaacggat gaacgagttg caggcgaggg tatggcaata ctggatgtaa
      841 ccaccacgcg ctgcatgctc tgctggtccc ggtcacttca gggtctgatg ccgatcattg
      901 cttcggaccc ggtgcggtag cggcgacttg cccggcgcgg ccgaggtggc gttgccggcg
      961 ccactcaggc accggcaaac accacttcga ccgaaaggca caaggaatcc atcccggatc
     1021 aaacgtcaga acgccgaagc ttcgagacgc caggcttcga gacgcccaag cttcgagacg
     1081 ccggagcttc gaacggcgcc gaagcccaac gtagaaacta ccggacgaag ccttccagtt
     1141 catcctgcgc ccagtcctgg acctgcttga tcgactgacc actctgcagc ttggccagca
     1201 tcgtcggcaa ggtgccgcga ttataggcct ggacggcgat ctccggcggg gcaggggcca
     1261 acgcgatcca gctctcttga tggtgcgact tgcggattgg gtagtgatag acggtgcctt
     1321 tcggcggccc gacctcttcc caaaccttga aatcggtcat gctggcgaag ggcgggacgt
     1381 cgtagccgag gctggcgttg caccgcgcct cgacattctc ccgctgcgag aggaactcga
     1441 tcaactcctt ggccgcggtc ttgttggagc taaacttcca gatcccccag gagaacgccc
     1501 ccatcgggat gaaacggccc ttcggtcctt tcggagccgg aaacgtccag caatcctcgg
     1561 ccacttttgg cgcgtcgcgc cgggctacgg cccaggccga aggcgggttc cagatcagcg
     1621 cgctcttgcc ggaaatcagc gcgcggttgt tcgatgcgtc gtcatagctg acggcatccg
     1681 ccggcaggtg tttcaccaat tgctgcgcgt attccagcac ctggcggacg ttatcggacc
     1741 gcacagtgat gtcgcccttg gcgctgatca cttcggctcc gaatgccgcg aacatcgctc
     1801 cggcggtatc gaccgagtcc ggggttgtgc tcagcccgat gccgaacgtc atgttggctt
     1861 tcttgcaggc ctccgcggcc ttcagcatcg tgtcgtaggt ccagttttcc gcttccgggc
     1921 cggcctcggc g
//
LOCUS       DASWQN010000753         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_337225, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000753 DASWQN010000000
VERSION     DASWQN010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337225"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            751..966
                     /locus_tag="VGC82_12760"
     CDS             751..966
                     /locus_tag="VGC82_12760"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEY0424184.1"
                     /translation="MIGGFARAHVSSLTEADLDALEAVMEFPDTDLADWLTGRVPIPA
                     EEDTPMLRRMREFQTHRLRDRRDSQTP"
     gene            963..>2125
                     /locus_tag="VGC82_12765"
     CDS             963..>2125
                     /locus_tag="VGC82_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY0424185.1"
                     /translation="MSDAIKVWGAPEGWDAFLLAKRRREHSGSILHVTRDDARMARLA
                     EALAFVMPEAEILRFPAWDCLPYDRVSPQAALVSERIATLARLLEKPSGPRIVLTTVN
                     ALVQRVPPRSAFAGASMDLTVNGQVKPEKLAEYLEANGYGRAGTVMEPGEYAMRGGII
                     DIFPSGEPDPVRLDLFGDTIESIRVFDASSQRSAGKRKSLSLRPVSEVPLGKESIARF
                     RTGWRELFGQEAASDPIYLSISDGRRHPGMEHWVPLFHPTMENLLDYLPDAAVSLDHQ
                     SEEVLEARLEMIADHAQARKAVPRDGEVPYRPIPPSMLYLDREGWDEMLEYGPLLAFT
                     PFGMPDGAGGVDGGGRPGPVFAQSGGGPGVTVFDQLRGQAERWNTEGRRIVVAA"
BASE COUNT          361 a          664 c          720 g          380 t
ORIGIN      
        1 cgggccgatg ccacgcaagc ttgtcaacgg cgcgaagagc ggggcaagcg tttcggcatc
       61 cgacggttgg gcattcgcag gccctgcgtt cggttgccga gaatccaggg tccgtctgca
      121 gatcgccgct ttgggtcgag cgcgcgtcgg tagatcggtc atggcgggcg tgggcccaca
      181 agccacgcct gggccagatt cagggtccgg tcatgattaa cttctccggt tggtggacgg
      241 ccccgccacc ccgtcctgga gggcaaaggc ccatcatcca cggctgtgcc ggatttggcg
      301 cctgtcgaaa aaaaatcgct gcggccttcg atcgccatca cggacttgag atcgtgcttg
      361 gagcggtcag taatcaacgg accgttagca ctgcgagccg tggatcgtgg gtttttgccc
      421 accaagacgg tatccgagac cccgcccgca gcagctattt cggagcagac gcttggcacc
      481 gcgagccgtg ggtggtgggt ctgcgcccag catgacgggg atgaccggat cgtgcacaaa
      541 tcggagccgt cgttccgaac cggacccctt ggggctgaac attttacgag atcggcaact
      601 acaggccggg cgttcacggg ctgacagata tggcgggcac ctctatatga cagccgatcg
      661 atgactgaac cagaccactc agcgctggac ataagacgcc ggcggctttt gtttcgcgcc
      721 acgcatcgcg ggacgtttga aaacgatctg atgatcggtg gattcgcgcg tgctcacgtc
      781 tccagtctaa ccgaagcgga tctggatgcg ttagaggcag tcatggagtt tcccgatacc
      841 gatctggccg actggctgac gggccgtgtg ccgatccccg ccgaagagga cacgccgatg
      901 cttcgccgca tgcgggaatt ccagacgcac aggcttcggg acaggcggga ctcgcaaaca
      961 ccatgagcga cgcgatcaag gtttggggcg ctccggaagg ctgggacgct ttcctgctgg
     1021 cgaagcgccg gcgcgaacat agcggcagca tcctgcatgt cacccgcgat gatgcccgca
     1081 tggcgcgctt ggccgaggca ctggcgttcg tcatgccgga agccgagatc ctgcgctttc
     1141 cggcctggga ctgcctcccg tatgaccgcg tctcccccca ggcggccctg gtttccgaac
     1201 ggatcgcaac cctggctcgg ctgctggaaa aacccagcgg accgcggatt gtcctgacca
     1261 cggtcaacgc cctggtgcag cgcgttccgc cacgctccgc cttcgcgggc gccagcatgg
     1321 acctgacggt caacggccag gtgaagccgg agaaacttgc cgagtacctg gaggccaacg
     1381 gttatggccg agcggggacg gtgatggagc cgggcgagta tgcgatgcgc ggcgggatta
     1441 tcgacatctt tccgtccggc gaacccgatc cggtgcgcct ggacctgttc ggcgacacga
     1501 tcgagtcgat tcgggttttc gacgccagtt cgcagcgcag cgccggcaag cgcaagagcc
     1561 tctccctgcg tccggtttcc gaggtgccgc tgggcaagga gtcgatcgcc cgtttccgca
     1621 ccggctggcg cgagctgttc ggccaggagg cggcatccga cccgatctac ctgtcgatct
     1681 ccgacgggcg ccggcatccc gggatggagc attgggtgcc gctgttccat ccgacgatgg
     1741 aaaacctgct cgattacctg ccggatgccg cggtcagcct ggaccatcaa tccgaggagg
     1801 tcctggaggc tcggctggag atgatcgccg accacgccca ggcccgtaaa gccgtgccgc
     1861 gggatggcga ggtgccctat cggccgatcc cgccgtcaat gctgtatctc gaccgcgagg
     1921 ggtgggacga gatgctggag tatggtccgc tgctcgcctt caccccgttc gggatgccgg
     1981 acggcgccgg cggcgtcgat ggcggcggcc ggccgggtcc ggtattcgcg cagtcgggtg
     2041 ggggtcctgg cgttaccgtc ttcgaccagc ttcgcggcca ggcggagcgt tggaacaccg
     2101 aggggcggcg aattgtcgtt gccgc
//
LOCUS       DASWQN010000754         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_337412, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000754 DASWQN010000000
VERSION     DASWQN010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337412"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VGC82_12770"
     CDS             complement(<1..203)
                     /locus_tag="VGC82_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424186.1"
                     /translation="MFPDLKFAATNEHQVSSNQSFGKCITRLQRLSGLRIHQCPESEV
                     RHIRRTSVSRHPLFVFSGLLIPRV"
     gene            complement(442..1482)
                     /locus_tag="VGC82_12775"
     CDS             complement(442..1482)
                     /locus_tag="VGC82_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HEY0424187.1"
                     /translation="MPYPTHLLPTTVVGSYPQPGWLVNPNTLHQHGVPRAHAHDMWRV
                     PEALLEQAQDDATILAIRDMERAGIDIVTDGEIRRESYSNRFALALEGVDADTPGELR
                     SQAGRVTKVPRVVGKIRRISGVETRDALFLRENATHATKITLPGPFTLSRQVQNDFYQ
                     DEEELAMDYAIAVNQELHALKATGVDVVQLDEPWVRTAPDKAIRYGIRAINRALQGID
                     GPTVVHVCFGYAAVVSEKPSGYSFLAELGDTVAQQISIEAAQPRLDLGVLRDLAGKTI
                     MLGVLDLGDPAVELAETVAERIREGLRFVPADKLIPAPDCGMKYLARDVAFGKLRALA
                     DGAAIVRRELAG"
     gene            1566..2450
                     /locus_tag="VGC82_12780"
     CDS             1566..2450
                     /locus_tag="VGC82_12780"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY0424188.1"
                     /translation="MWPIRSALFVPAHRRDWVAKAIRVAPGAAVLDIEDSVPPQFKSQ
                     AMAFLEAEIHELRDAGVGAFVRIQPLFDGTAAEVEAAVTDGLTAIVLPKVAAPDEIRR
                     LHDLLSYAEGRAGIAHGSVGILPLPETAQGMYDAYNLAKASPRVRGIHGALSGAVSGD
                     FARAFGFRATAGGEEQQYLASKLVLDSRAAGAMHPIAGIFGTAQDDLPAVERLIRKAR
                     DFGYSGVAVMHPSHVAIANAAYRPTAEEVAYFEGLLEAFAAAEKAGLGAVSYQGAMVD
                     YAMLPLAKEVLAEAARIR"
     gene            2488..>3561
                     /locus_tag="VGC82_12785"
     CDS             2488..>3561
                     /locus_tag="VGC82_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY0424189.1"
                     /translation="MAADSISTDAAPRAIRLADYAPPAFLIDTVDLNFELDETATKVV
                     AKLALRRNPAAPPQSPLHLDGEALTLVRITRDGSTLAPAAYQATKGALTIPDMPDVCV
                     LEIETRLSPKDNTELSGLYVSGGNYFTQCEAQGFRRITYFPDRPDVMSRYTTTIVGPC
                     PVMLSNGNPGPVTDLGDGRRSVTWVDPHPKPCYLFALVAGDLVAVNDSFVTRSGRDVK
                     LGIYVRRGDEDRCGHAMRSLKTSMKWDEDVFGLEYDLDVFNIAAVSDFNMGAMENKGL
                     NVFNTKYVLARPETATDSDYQGIETVIAHEYFHNWTGNRITCRDWFQLTLKEGLTVFR
                     DQEFSADMSSGFAEGREDATAAAV"
BASE COUNT          662 a         1163 c         1099 g          637 t
ORIGIN      
        1 acgcggggga tcagcaggcc actgaaaaca aagagagggt ggcgcgagac tgaggtccgc
       61 cggatgtggc ggacttcaga ttcggggcac tggtgaatac gcaggccgga caaacgttgc
      121 agacgcgtga tgcacttacc aaaggactga ttcgacgaaa cctgatgttc gtttgttgcc
      181 gcgaacttca ggtccggaaa caacagctca agtcaataaa gtgagccggc cagtgacgaa
      241 tctggccggc gtcctgctta gggtctgctc cgaaatattc gcctcggtca cgatctccaa
      301 tccagcaacg atcgcgatgg cgactcaact ggcgtacatc gggcaaagcc cgttatgacg
      361 ttgctgagtt gtaccacccg aactcgaagc gcttggcaac aaaccttggc ttaaaccaga
      421 acatcgattg acggccggac cctatccggc caactctcgc cgtacgatcg ccgcgccgtc
      481 cgcgagtgcc cgcagttttc cgaaggctac atcgcgcgcc aaatatttca ttccgcaatc
      541 cggcgccgga atcaatttgt cagccggcac gaagcgcagc ccctcgcgaa tgcgctcggc
      601 aaccgtctcc gccagttcga cggccggatc gcccagatcc agcacgccca gcatgatcgt
      661 cttgccggcc aaatcgcgca ggacgcccag gtccaaccga ggctgcgccg cctcaatcga
      721 aatttgctgt gcaacggtat cgccgagttc cgccaggaag ctgtagccac tgggcttttc
      781 cgagaccacc gcggcgtagc caaagcatac gtgcaccacc gtcggaccgt cgatcccttg
      841 cagcgctcgg ttgatcgcac gtatcccgta acggatcgcc ttgtcgggcg ccgtgcgcac
      901 ccagggttca tccagttgca ccacatcgac gccggtcgcc ttcagcgcgt gaagttcctg
      961 gttcacggcg atggcgtaat ccatcgcgag ctcttcctcg tcctggtaga aatcgttctg
     1021 cacctggcgt gacagcgtga atgggccggg cagcgtgatc ttcgtggcgt gggttgcgtt
     1081 ctcccgcaga aacagcgcgt cccttgtctc cacgccggat atccggcgga tcttgccaac
     1141 cacgcgcggc accttggtga cccgtccggc ctgggagcgc aactcgcccg gggtatcggc
     1201 gtccacgcct tccagcgcga gcgcaaagcg gttcgaatag ctttcccgcc tgatttcacc
     1261 atcagtgacg atgtcgatcc cggcgcgctc catgtcgcgg atcgccagga tcgtcgcatc
     1321 gtcctgcgcc tgctccagca gcgcctccgg cacgcgccac atgtcatgcg cgtgggcacg
     1381 aggcacgccg tgctggtgca acgtgtttgg gttcaccagc cagccgggct gaggatagct
     1441 gcccacgacg gtggtcggca gcagatgggt gggatagggc atggaacgga acctcctgtg
     1501 gcacgattgc accaccgggc gtgtccccgc aaactccgga ccatacgctc caggagaatg
     1561 atcgcatgtg gcccatccgt tccgcgttgt tcgtgccggc gcatcgccgc gactgggtgg
     1621 cgaaggcgat ccgcgtagcc cctggcgcag ccgtgctgga catcgaggat tccgtgccgc
     1681 cccagttcaa gtcgcaggcg atggccttcc tggaagccga gatccacgaa ctgcgcgacg
     1741 ctggcgtcgg cgcttttgtt cgcatccagc ccttgttcga cggcacggcc gccgaggtcg
     1801 aagcagcggt aaccgacggg ctgaccgcca tcgtattgcc aaaggtcgcc gccccggacg
     1861 agatccgccg gctacacgat ctgctgagct acgccgaggg ccgcgccggc atcgcccatg
     1921 gcagcgtcgg tatcctgccg ctgccggaaa cggcccaggg catgtacgac gcctataacc
     1981 tcgcgaaggc cagcccgcgt gtgcgtggca tccacggcgc gttgagcgga gcggtgtccg
     2041 gcgacttcgc gcgtgccttc ggttttcgag cgacggccgg cggcgaggag caacagtatc
     2101 ttgcctcaaa gctggtgctg gacagcagag ccgccggcgc aatgcatccg atcgccggca
     2161 ttttcggcac ggcgcaggac gatctgccgg ccgtcgagcg actgatccgc aaggcgcgcg
     2221 acttcggata ttctggcgtc gcggtcatgc atccgtccca tgtcgcgatc gccaatgcgg
     2281 cgtatcgccc gaccgccgag gaggtcgcct acttcgaggg cttgctggag gcattcgcgg
     2341 cggcggagaa agccggcctc ggcgcggtca gctaccaggg cgcgatggtc gattacgcga
     2401 tgctgcccct ggcgaaagag gttctcgccg aggccgcccg gatccgatag catccagtat
     2461 catgcacgac ctgaagccgg agaacacatg gcagccgact ccatcagcac cgacgccgca
     2521 ccgcgtgcca tccggctggc ggactacgcc ccgccggcct ttctgatcga caccgtcgac
     2581 ctgaactttg aactggacga aaccgccacc aaggtcgtcg caaagctggc gctacggcgc
     2641 aatcctgccg cgccgccgca atcgccgctg catctggacg gcgaggcgct gacgctggtc
     2701 cgcatcaccc gcgacggttc gacgctcgct ccggcggcct atcaagcgac caagggcgca
     2761 ctgaccatcc cggacatgcc ggacgtctgc gtgctggaga tcgagacgcg gctgtcgccg
     2821 aaggacaaca cggaactcag tggcctttac gtatcgggcg gcaattattt tacccagtgc
     2881 gaggcgcagg ggttccggcg gatcacctat tttcccgata ggcccgatgt aatgtcgcgc
     2941 tataccacga ccatcgtcgg gccttgcccc gtgatgctgt cgaacggcaa cccgggtccg
     3001 gtcaccgatc tcggcgatgg ccggcgatcc gtcacctggg tcgatcctca tccgaaacct
     3061 tgttatctgt tcgcgctggt ggcgggcgac ctggtcgcgg tcaacgacag tttcgtcacc
     3121 cgctccgggc gtgacgtgaa gcttggcatc tacgtccgcc gtggtgatga agaccgctgc
     3181 ggccatgcga tgcgctcatt gaagacgtcg atgaagtggg acgaggacgt ttttggcctg
     3241 gagtacgacc tggacgtgtt caacatcgct gccgtgtccg acttcaacat gggcgcgatg
     3301 gagaacaagg gcctgaacgt cttcaacacc aaatacgttc tcgcgcgtcc ggaaaccgcg
     3361 accgacagcg actaccaggg catcgaaacc gtcatcgcgc acgagtattt ccacaactgg
     3421 accggcaacc gcatcacctg ccgcgactgg ttccagctga cgctgaagga agggctgacg
     3481 gtctttcgcg accaggaatt ttccgccgac atgagctcgg gctttgccga aggacgcgag
     3541 gatgcgacgg cggccgcggt c
//
LOCUS       DASWQN010000755         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2316084, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000755 DASWQN010000000
VERSION     DASWQN010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2316084"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1052)
                     /locus_tag="VGC82_12790"
     CDS             complement(186..1052)
                     /locus_tag="VGC82_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0424190.1"
                     /translation="MAIRKLLLPLTGTAAGEAALATALTIARRWNAHVLALHVRVDSR
                     DVAPLAGEGLSGAMIEEMMSATEKESNDRAHAVRSMFERFVARHDVAVQEAHAGSDHA
                     TASFAAVTGREEDIVAQQARLADLTVVPHPDSGEDVSSSDALHAVLFDSGRPVLIAPQ
                     VTPPMIGARVCVAWNGTAESASSVQGALPWMQRAESVAILTAEGYQRRGPGAPDLAAY
                     LALHGIRPEIVTFKSVSGSVGAGLLAAAANFGCDLLSMGAYSHSRLRQLILGGVTRHV
                     LEHSTLPVMMNR"
     gene            complement(1293..1889)
                     /gene="pdxH"
                     /locus_tag="VGC82_12795"
     CDS             complement(1293..1889)
                     /gene="pdxH"
                     /locus_tag="VGC82_12795"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY0424191.1"
                     /translation="MPAEDFLAVSPYAQFYAWMAEAETSEPNDPNAMAVATATPDGRP
                     SVRTVLLKGVDERGFVFYTNKESRKGEELSLNPNAALLFHWKSLQRQIRIEGTVEPVT
                     DAEADAYYSSRARISRLGAWASLQSRPLAKRTILQERLAEMEARYPDVIPRPSYWSGY
                     RVLPEMFEFWQDMPYRLHDRTIYRRSAGGVWEQGKLFP"
     gene            2051..3217
                     /locus_tag="VGC82_12800"
     CDS             2051..3217
                     /locus_tag="VGC82_12800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0424192.1"
                     /translation="MKETLRADPHAEIREEVRKLCSRFPGEYWRKLDQERGYPTDFVR
                     ALTEGGYLAALIPEDYGGAGLPLSAAAAILETVQAEGSNGAACHAQMYIMGTILRHGT
                     DQQKKAYLPKIASGELRLQAFGVTEPTSGTDTTSLRTFARKEGDTYVVNGQKVWTSRA
                     EHSDLMLLLARTTPKDQTTKRTDGLSTFIVDMRAALGHGLTIRPIRTMMNHNSTEVFF
                     DNMVIPAENLVGVEGRGFRYILDGMNAERLLIAAECIGDAKWFLRKASDYARERQVFG
                     RPIGQNQGIQFPLARAYAQMRAAELMVQAGCEKFEAGETPGEEANMSKMLAAEASWAA
                     GEACIQTHGGFGFAEEYDIERKFRETRLYQVAPISTNLILAYIAEHVLGMPRSY"
BASE COUNT          673 a         1093 c         1026 g          568 t
ORIGIN      
        1 cacgcgatca aaccaagagc ccggagcgtg tgaagcacgc gatcaaacca agagtccgaa
       61 gcgtgtgaag cacgcgatca aacaaagaga caaagctttt tcggcctgta caggcaggct
      121 ggaaatgtca tagtccgggt cggaagctcg ccatgccggc gaacctccgc tccgacagcc
      181 agcggtcagc ggttcatcat caccggcaag gtggaatgtt ccagcacatg gcgggtcact
      241 ccaccaagga tcaactgacg cagccgggaa tgcgaatagg cgcccatcga tagcaggtcg
      301 cagccaaaat tggccgccgc cgccagcagg cccgccccga ccgatccgct gaccgacttg
      361 aatgtaacaa tttccggacg tatgccgtgc agcgcgaggt aggctgccag atccggcgca
      421 cccgggccgc gcctttgata gccttcggcc gttagaatgg cgacgctttc ggcacgctgc
      481 atccacggca gcgcgccctg gaccgacgat gccgactccg cggtgccgtt ccatgcgacg
      541 caaacccgcg cgccgatcat cggcggcgtt acctgaggcg cgatcagcac cggccggccg
      601 gagtcgaaca gcaccgcgtg cagtgcgtcc gacgacgaca cgtcctcacc ggagtcggga
      661 tgcggcacga cggtcaggtc ggccaaacgc gcctgctgcg ccacgatgtc ctcttcacgg
      721 ccggtcaccg ccgcgaaact ggccgtcgcg tgatccgaac ccgcatgggc ttcctgaacg
      781 gccacgtcgt gacgggcaac gaaacgttcg aacatcgagc ggaccgcatg cgcccggtcg
      841 ttgctttcct tctcggtcgc cgacatcatt tcctcgatca tcgcaccgga caggccctcg
      901 ccggccagcg gcgccacgtc ccgactgtca acccgtacgt gcaatgcgag aacatgggcg
      961 ttccatcggc gggcaatggt gagcgcggtt gccagtgcgg cttccccagc cgcggttccg
     1021 gtcagaggca gcaaaagttt gcgtattgcc atggtcagct ccgcaaaagg gttttgcgcc
     1081 cagcgttccg aacctgacgt tcgccatcat gagcgaaatt gaggttcggg aacactagtt
     1141 ctttgttttc agtaacctgc gatcccgaga gtcctcggtg cgacgaacct tggggtgcga
     1201 cgaactttgg gagcaggact acaagagcaa ggcaggtttg ctttggggtt gcaagtacat
     1261 tctgggcaag tgcgtccgac gaagcgacga gcctacggga agagttttcc ttgctcccac
     1321 acgccgcccg ccgaccgccg atagatcgtc cggtcgtgca aacgataggg catgtcctgc
     1381 cagaactcga acatctccgg caaaacccga tatccagacc aatagctcgg tcgcggaatt
     1441 acatctgggt aacgcgcctc catttccgca agacgctcct gtaaaattgt tcgcttcgcc
     1501 aacggacgcg actgcagcga tgcccaggca ccgagccggg aaattcgcgc ccgtgagctg
     1561 tagtaggcgt cggcttccgc atcggtgacc ggctcgaccg tgccctcgat acggatttgc
     1621 cgttgcaacg atttccagtg aaacaacagc gccgcgttcg gattgagcga cagttcctcg
     1681 cccttgcggc tttccttgtt ggtgtagaaa acgaagccgc gctcatcgac gcccttcagc
     1741 agcacggtcc gcaccgaggg acggccatcc ggcgtagcgg tcgccacggc catggcgttc
     1801 ggatcgttcg gctccgacgt ttccgcttcc gccatccatg cgtagaactg cgcgtatggc
     1861 gaaaccgcca aaaaatcttc agccggcatg aatcaggccc tcacatcgtg acatccggca
     1921 ctgataacag catgttgctg gaatccggca agcacccatc cggtgccggc cctcgtacac
     1981 ctcggccgct cgtcggttgc cggccggccg gcaaacccaa tactgagaaa acccgttcgc
     2041 ggaggccgcc atgaaagaga ctttacgcgc cgatccacac gcggaaatcc gcgaagaggt
     2101 ccgcaagttg tgcagccggt ttcctggcga gtactggcgt aagctcgatc aggagcgcgg
     2161 ctatccgacc gatttcgtgc gcgcgctgac cgaaggcggg tatctcgccg cgctgattcc
     2221 cgaggattac ggcggcgccg gcctgccgct gtccgccgcc gcggcgatcc tggagacggt
     2281 gcaggccgag ggcagcaacg gtgcggcctg ccacgcccag atgtatatca tgggcactat
     2341 cctgcggcat ggcaccgacc agcagaagaa ggcctacctg ccgaagatcg ctagcggaga
     2401 gcttcggctg caggcattcg gcgtcaccga acccaccagc ggaaccgaca cgacgtcgct
     2461 gcgcacgttt gcccgcaagg aaggcgacac ctacgtcgtc aacgggcaga aggtgtggac
     2521 cagccgggca gaacattccg acctgatgct gctgctggcg cgaaccacgc ccaaggacca
     2581 gacgaccaag cgcaccgacg gtctttcgac cttcatcgtc gatatgcgcg ccgccctcgg
     2641 ccacggcctg accattcgcc cgatccgcac catgatgaac cacaactcga ccgaggtgtt
     2701 cttcgacaac atggtcatcc cggccgagaa cctcgttggc gtcgagggcc gtgggttccg
     2761 ctacattctg gatggcatga acgccgaacg gctgctgatc gccgcggagt gcatcgggga
     2821 cgccaagtgg ttcctgcgca aggcctccga ctatgccagg gaacgccagg tgttcggccg
     2881 gccgatcggc cagaatcagg gcatccagtt tccgctggcc cgcgcctacg cgcaaatgcg
     2941 cgccgccgaa ctgatggtgc aggcgggttg cgaaaaattc gaggccggag agacgcccgg
     3001 agaggaggca aacatgtcca agatgctggc ggccgaggcc tcatgggcgg cgggcgaggc
     3061 ctgcatccag acgcatggcg gcttcgggtt cgccgaggaa tacgacatcg agcggaagtt
     3121 ccgtgaaacc cggctgtatc aggtggcgcc gatcagcacc aacctgatcc tggcctatat
     3181 cgccgagcac gtgctcggca tgccgcggag ctattgatgc gaacgatggt cgttccatac
     3241 cggactgcgg ccgacaggca tctaggtgca actgccacgg cgcgatcccc ctcccctaca
     3301 cccctccccc gcacgcggga gaggggaacg gcgggtcgtg tctggttgtt tccagggccg
//
LOCUS       DASWQN010000756         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2052815, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000756 DASWQN010000000
VERSION     DASWQN010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052815"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VGC82_12805"
     CDS             <1..492
                     /locus_tag="VGC82_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY0424193.1"
                     /translation="IERGLKMTGQRRVVAKVLSEAGDHPDVEELYHRASALDQHISIA
                     TVYRTVRLFEEQGILERRDFGGGRARYEPTEDGPHYHLIDVETGKVIEFQDPEHERLM
                     RVIAARLGFDVVSLRLELFGRRHGSAVAPHEGGLSEHEGSHDSSAASRDERFGDPIKG
                     ASQ"
     gene            489..1319
                     /locus_tag="VGC82_12810"
     CDS             489..1319
                     /locus_tag="VGC82_12810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003623791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEY0424194.1"
                     /translation="MTSGTSAVESVVRPFAEIRSGNLGVRITADAKEIDALQALRYRV
                     FYQEMGARPDAVTATTRRDRDIYDTVADHLLVVDHSLGEGAESVVGTYRLIQRHAASQ
                     IGHFYSSDEYDISRIEACPGPILELGRSCVDNLYRNRAVMQLLWRGIAAYVFQNKIEL
                     MFGCASLPGTDPDAIAAELTYLYRNHLAPDAIRPRALPDRYIDMCRLGQEDIDIRAAL
                     TKLPPLLKGYLRLGGFIGDGAVVDPQFNTTDVAVVVQTDLVTDKYSKHYELQRRETSD
                     "
     gene            1327..>2612
                     /gene="lnt"
                     /locus_tag="VGC82_12815"
     CDS             1327..>2612
                     /gene="lnt"
                     /locus_tag="VGC82_12815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY0424195.1"
                     /translation="MPPLIRRLRGLHGWRADLVALASGALSALALPPLFVLPALLAGI
                     PVLLCLIQGARGPAVAARRGWWFGFGLHLVGLYWITEAILIEAARFWWLVPFAVPALA
                     AVLAVFTAVPAAVTWYAGSAERGPAWSGWRVAATLAGTWVLSDLARQFVATGFPWNPL
                     GSVWEFPGRVGDIMIQPASLFGAHGLTLVTILLASLPLLGWGWRAGGLAVLALWCGFG
                     LNRIDQKMPPGPDLTVLLIQGNVAQGQKWDQALMVSIFRRYLALTGQAVGKANGHPAV
                     VVWPETASPAQLQTDSQARKLIAEAANGAPALIGSVRFDDASRPRNSLFALGAQGQIE
                     AIYDKWHLVPFGEYQPDWLPLGIQVVPGGGFARGPGPRTLHVPGLPAVGVLICYETIF
                     PSEVVDPSDRPDWMVNVTNDAWFGNSSGPRQHLAAA"
BASE COUNT          420 a          806 c          876 g          510 t
ORIGIN      
        1 atcgaacggg gcctgaagat gaccggccag cgcagggttg tcgcgaaggt cctgtcggag
       61 gccggcgatc acccggatgt ggaggagctt taccataggg cttccgcgct tgatcagcac
      121 atctcgatcg cgacggttta ccggacggtt cgcctgttcg aggagcaggg catcctggag
      181 cggcgggatt ttggcggcgg ccgagcccgt tacgaaccca cggaggatgg cccgcactac
      241 cacctgatcg atgttgaaac cggcaaggtc atcgaattcc aggatccgga acatgagcgg
      301 ctgatgcgcg tgatcgccgc gcgcctcggt tttgacgtcg tttccttgcg gctcgaacta
      361 tttggccggc ggcacggttc ggcggttgcc ccgcatgagg gtggcttgtc cgagcacgag
      421 ggttcgcacg actccagcgc tgccagccgt gacgaacgat tcggcgaccc gatcaaagga
      481 gcgagccaat gacctcgggc accagtgccg tcgaatcggt cgtgcggccg ttcgcggaga
      541 tcaggagcgg caaccttggc gtgcggatca ccgccgacgc gaaggaaatc gacgcccttc
      601 aggcgctgcg ctaccgggtg ttctaccagg aaatgggcgc ccgtcccgat gcggtaaccg
      661 ccaccacccg gcgcgaccgg gatatctacg acaccgtggc cgaccatctg ctggtggtcg
      721 atcacagcct gggcgaggga gcagaaagcg ttgtcggcac ctaccggctt attcagcgcc
      781 acgctgccag tcaaatcggg catttctatt cgtcggacga atacgacatc agccgcatcg
      841 aggcttgccc gggccccatt ctagaactcg gccggtcctg tgtggacaat ctgtaccgca
      901 accgcgcagt gatgcagttg ctgtggcgcg gcatcgctgc ctacgtgttt cagaataaga
      961 tcgagctgat gtttggatgc gccagtttgc ccggcaccga ccccgatgcc atcgcggcgg
     1021 agctgacata cttgtaccgc aaccacctcg ccccggatgc gatccggccg cgtgccctgc
     1081 cggaccgcta tatcgatatg tgccgcttgg gccaggagga tattgatatc cgcgcggcgc
     1141 tgaccaaatt gccgccactg ctcaagggtt atctgcggtt gggcggtttc attggcgacg
     1201 gagcggtggt ggacccgcaa ttcaatacga ccgatgtcgc ggtcgtggtg cagaccgatc
     1261 tggtaaccga caaatactct aaacactatg aactccagcg ccgcgagacg tccgactagc
     1321 catgccatgc cgcctttgat tcgccggttg cgtgggcttc atggctggcg tgccgatctg
     1381 gtcgctttgg cgtcgggcgc attgtcggcg cttgcattgc cgccgttgtt cgtgctgccc
     1441 gccttgctgg cggggatacc ggtgttgctt tgcttgatcc aaggcgctcg cggtccggcc
     1501 gtcgccgctc ggcgaggctg gtggttcggc ttcggcttgc atcttgtagg cctgtactgg
     1561 atcaccgagg caatcctgat cgaggccgct cgcttctggt ggcttgtgcc gttcgcggtg
     1621 ccggcgctgg ccgccgtgct cgcggtgttc accgcggttc ccgccgcggt gacctggtac
     1681 gccggctccg ccgagagggg accagcttgg tccggctggc gagtcgcggc cactttggct
     1741 ggtacctggg tgctgtcgga ccttgcgcgt cagttcgtgg cgaccggctt tccttggaac
     1801 ccactgggaa gcgtgtggga gtttcccgga cgggtaggcg acatcatgat ccagcccgcg
     1861 tcattgttcg gggcgcatgg actgacgctg gtgacgatcc tgctggcctc gctgccgttg
     1921 ctgggctggg gctggcgggc cggcggcttg gccgtcctgg cgttgtggtg cggttttggc
     1981 ctgaaccgga tcgaccagaa gatgccgccg gggcccgatc tgaccgtgct gctgatccag
     2041 ggcaacgtcg cgcagggcca gaaatgggac caggcactga tggtctcgat cttccgccgc
     2101 tatctggccc tgacaggaca ggcggtaggg aaagcgaacg gccatcccgc ggttgtggtc
     2161 tggccggaga ccgcaagccc ggcgcagttg cagaccgaca gccaggcccg caaactgatc
     2221 gccgaagcag ccaacggcgc gcccgccttg atcggttcgg tgcggttcga tgatgcgagc
     2281 cgcccgcgca acagcctgtt tgccctgggc gcccaaggcc agatcgaagc catatacgat
     2341 aaatggcacc tggttccatt cggggaatat cagccggact ggctgccctt gggtatccag
     2401 gtggtaccgg gcggcggctt cgcccgaggc cctggtccgc ggacattgca cgtgccggga
     2461 ctgcccgcgg tcggcgtgct gatctgctac gagacgatct ttcccagcga agtggttgat
     2521 cccagcgacc ggcccgactg gatggtgaac gtcaccaacg acgcgtggtt cggcaattcg
     2581 agcggcccac ggcagcatct ggctgccgcc ag
//
LOCUS       DASWQN010000757         5903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_468987, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000757 DASWQN010000000
VERSION     DASWQN010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468987"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="VGC82_12820"
     CDS             complement(<1..147)
                     /locus_tag="VGC82_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HEY0424196.1"
                     /translation="MRVIGLAGWSGAGKTTLVRRLIPALIARGLRVSTLKHAHHSFDI
                     DQPGK"
     gene            282..2027
                     /locus_tag="VGC82_12825"
     CDS             282..2027
                     /locus_tag="VGC82_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY0424197.1"
                     /translation="MTEADAGKPNAGKIIRFEHPGAGRQRAKTTPKGREVQPWAKDEV
                     ARLLADRPRAREFLIEYLHLIQDTYKQISAAHLAALADEMRLAYAEVFETATFYAHFD
                     VVKEGDPPLAPLVIRVCDSVTCAMFGAERLLKELQALPDANGVGAKARVVRAPCVGLC
                     DQAPVVEVGHHFLHNADQTSVQKAIERGDTHPHVPDDYIDYHVYVAAGGYRLLHQIRS
                     GEIDVETVLSKLDDTGLRGLGGAGFPTARKWRSVRGEPGPRLMAINADEGEPGTFKDR
                     HYLNSDPHRFLEGMLIAAHVVDARDIYIYLRDEYPVAREILTRELAELPAGGPIVHMR
                     RGAGAYICGEESSMLESLEGKRGLPRHKPPYPFQFGLFGRPTLINNVETLYWVRDLLE
                     KGVSWWTGFGRHGRTGQRSFSVSGRVKQPGVKLAPAGITAQELIDEYCGGMLDGHTFT
                     AYLPGGASGGILPASMADIPLDFGTLEKHGCLIGSAAVVVFSDQDDIKGVALNLMRFF
                     EDESCGQCTPCRSGTQKARILMERDDWNVELLDELSQVMRDASICGLGQAASNPLTCI
                     IRYFPEKLAPKEIAE"
     gene            2024..4855
                     /gene="fdhF"
                     /locus_tag="VGC82_12830"
     CDS             2024..4855
                     /gene="fdhF"
                     /locus_tag="VGC82_12830"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEY0424198.1"
                     /translation="MSSSTTREGPQHLEATTTETQGLAFELDGERAEARPGETLWDVA
                     RRLGTNIPHLCHRPQPGYRPDGNCRVCMVEIEGERVLAPSCKRMPAVGMKVHSQSARA
                     VKARAMVMELLVADQPARETSHDPTSSFWSWAERTGVTESRFPAAERWTHDPSHPAMR
                     VNLDACIQCNLCVRACREVQVNDVIGMAYRNAGSKVVFDFDDPMGESTCVACGECVQA
                     CPTGALMPAAYLDENESRVVWPDRKVDSLCPYCGVGCQVTYEVANEKLVYASGRDGPA
                     NHNRLCVKGRFGFDYIHHPHRLTRPLVRLPNTPKHAQDQVDPANPWTHFREATWEEAL
                     DRAAAGFSFIRDANGPKALAGFGSAKGSNEEAYLFQKLVRTGFGSNNVDHCTRLCHAS
                     SVAALMEGVNSGAVTAPFAAALDAEVIIVIGANPTVNHPVAATFIKNAVKERGAKLIV
                     IDPRRQMLSRHALHHLAFKPGSDVALLNALLHTIIEEGLTDDQYIAGYTEGYEELRER
                     IKEFSPERMEPVCGIPAQTLREVARLYARSRASIIFWGMGISQHVHGTDNARCLIALA
                     LVTGQIGRPGTGLHPLRGQNNVQGASDAGLIPMVFPDYQSVEKVNIREQFETFWKTEL
                     DPVRGLTVVEIMNAIHGGQIKGMYIEGENPAMSDPDLNHARAALAKLEHLVVQDLFLT
                     ETAFHADVVLPASAFAEKSGTFTNTDRRVQIARPVIDPPGEARQDWWIIQQVGQRLGL
                     DWAYAGPSDIFAEMAQVMPSLNNITWERLEREGAVTYPVDAPDQPGHEIIFYSGFPTE
                     SGRAKIVPANVLPPDEVPDDDYPMVLSTGRVLEHWHTGSMTRRSGVLDAIEPEAIAFM
                     SPKNLWRENLRPGDLIRIETRRGVVEVKVRSDRDVPEGMVFMPFCYAEAAINLLTNPA
                     LDPFGLIPEFKFCAARVTASVDSGGMMAK"
     gene            complement(5078..>5903)
                     /locus_tag="VGC82_12835"
     CDS             complement(5078..>5903)
                     /locus_tag="VGC82_12835"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY0424199.1"
                     /translation="SMLSLSSRPGSDARPQRHWKNASAKEAAGLIRPGATVAVGWLGD
                     SLATALADAFALNTQPNGLTIVYGATLGDGRNHGLNLLAHEGLVRRVVGGQWHPVFGL
                     QALAAANRIEAYSFPVGVIRRLFRDIAEGMPGHLSRSGLGTFTDPRNGGGRLNPRTWE
                     QLVRLVRPAGNDVLLFQGFPIDAALIGVAFMEGTAAITMTRDSMAIARAARKCGGIVI
                     AQMNRIGTLDRLPSEQVVVPDSLIDILVAADPRDRAWETFSPARAAWKRQPRRLTH"
BASE COUNT         1126 a         1862 c         1894 g         1021 t
ORIGIN      
        1 ctttcccggc tggtcgatgt caaagctatg atgagcgtgc ttcaaggttg agacccgaag
       61 gccgcgtgcg atcagcgcgg gaatcaggcg gcggaccagg gtcgtcttgc ccgcgccgct
      121 ccagcccgcc agccctatga cacgcatcgg taaattggag taggatcagc cgcgctggac
      181 atgaagaacc ggacctcgtt ggatgacgct ggttaaggct tcctgagcaa gcagacgacg
      241 caggtgatgg atatgacgtt caggaagcac gggaaacact tatgaccgag gcagatgctg
      301 gtaagccaaa tgctggtaaa ataatccggt tcgagcatcc gggtgcgggg cgccagcgcg
      361 ccaagacgac gccaaagggc cgtgaagttc aaccctgggc caaggacgag gttgccagac
      421 tgctcgccga ccggccccgc gcacgcgagt tcctgatcga gtatctgcac ctgatccagg
      481 acacctacaa gcagatctcg gcggcgcacc tggcggcgct ggcagacgaa atgcggctgg
      541 cctacgccga ggtcttcgaa acggcgacct tctacgccca tttcgacgtg gttaaggaag
      601 gcgatccgcc tctcgcgccg ctggtgatcc gcgtttgtga cagcgtgacc tgtgcgatgt
      661 tcggtgccga acggctgctt aaggagctgc aggcgttacc ggatgcgaat ggcgtcggtg
      721 ccaaggcgcg tgtcgtcagg gcgccatgcg tcgggctatg cgatcaggcg ccggttgtcg
      781 aagtgggcca tcatttcctg cacaatgccg atcaaacatc ggtgcaaaag gcaatcgagc
      841 ggggcgatac gcatcctcat gttcccgacg actacattga ctatcacgtt tacgtagccg
      901 ccgggggata ccgcctgctc caccagatcc gatccggcga gatcgacgtc gaaactgtgc
      961 tgtcgaagct ggacgacacc ggcctgcgcg gcctgggtgg cgccgggttc cctaccgcac
     1021 gcaaatggcg atcggtccgc ggcgaacctg gcccccggct gatggcgatc aatgccgacg
     1081 agggcgagcc cgggacgttc aaggatcgtc actacctcaa ctcagatcca caccgatttc
     1141 ttgagggaat gctgattgcc gcccatgtgg tggatgcgcg agacatctac atctatctgc
     1201 gcgatgaata tccggtggcg cgggagatcc tgacgcgcga gcttgccgag ttaccggccg
     1261 gcggcccgat cgtgcatatg cgccgcggcg cgggggccta tatttgtggc gaagagtcct
     1321 cgatgctcga gagcctcgag ggcaagcgcg gcctgcctcg ccataagccc ccgtatcctt
     1381 tccagttcgg cttgttcggg cgcccgacgc tgatcaacaa cgtggagacg ctgtactggg
     1441 tgcgcgacct gctcgaaaag ggcgtatcct ggtggaccgg cttcggccgc catggccgga
     1501 ccggacaacg gagtttctcg gtctccggcc gggtcaagca gccgggcgtg aaactcgctc
     1561 cggcaggcat caccgcccag gaactcatcg acgagtactg cggcggcatg ttggacggcc
     1621 acacgtttac ggcgtatctg cccggcgggg cctcgggcgg catcctaccc gcctccatgg
     1681 ccgacatccc gctcgatttc ggcacgctgg agaaacacgg gtgcttgatt ggctccgcgg
     1741 cggttgtggt tttctccgat caggacgaca tcaagggcgt cgccctcaat ttgatgcgct
     1801 tcttcgagga cgaaagctgc ggccagtgca cgccttgccg gtccggcaca caaaaggccc
     1861 gcatactgat ggaacgggac gactggaacg tcgagctgtt ggatgagctg tcgcaggtca
     1921 tgcgcgacgc ctcgatctgc ggattgggcc aggcagcctc taatccgcta acttgtatta
     1981 tacgctactt tccagaaaag ttggcgccga aggagattgc cgagtgagca gttcaaccac
     2041 acgcgaaggc ccgcagcatc tcgaagcgac cacgaccgag acgcagggcc tggcgttcga
     2101 actggatggc gaacgggccg aggcaaggcc gggcgagacg ctgtgggacg tggcacgccg
     2161 tttgggcacc aacattccgc atctgtgcca ccggccgcag ccggggtatc ggccggacgg
     2221 caactgccgg gtgtgcatgg tggaaatcga aggcgagcgc gtcctggccc catcgtgcaa
     2281 acgcatgccc gccgtcggca tgaaggtcca cagccagtcc gcccgcgcag tgaaggctcg
     2341 ggccatggtg atggaactgc tcgtcgccga ccagccagcg cgcgaaacct cacacgatcc
     2401 gacctcgtcg ttctggagtt gggcagagcg caccggcgtg accgaaagcc ggttccctgc
     2461 cgcggagcgc tggacgcacg accccagcca tcccgcgatg cgcgtcaatc tcgatgcctg
     2521 cattcaatgc aatttgtgcg tccgcgcctg ccgcgaggtg caggtcaacg acgtcatcgg
     2581 catggcatat cgcaacgccg ggtcgaaagt cgtgttcgat ttcgacgacc cgatgggcga
     2641 atccacttgc gtggcgtgcg gcgaatgcgt gcaggcatgc ccgaccggcg ccctgatgcc
     2701 ggcggcttat ctcgacgaaa acgagtctcg ggtggtttgg cccgaccgca aggtggacag
     2761 cctgtgcccc tattgcggcg tgggctgcca agtcacctat gaagtcgcaa acgaaaaact
     2821 ggtttatgcc agcggccgcg acggtccggc gaaccacaac cgtctgtgcg tgaagggccg
     2881 cttcgggttc gactatatcc atcacccgca ccggctcacg cgtcccctcg tccgcttgcc
     2941 gaacactccc aagcacgccc aggatcaggt ggaccccgcc aatccctgga cgcatttccg
     3001 cgaagcgaca tgggaggaag cgctggatcg cgccgccgcc ggcttcagct tcatccggga
     3061 tgcaaatggg cctaaggcac tggctggttt tggttcggcc aaaggctcga acgaggaggc
     3121 ctacctgttc cagaagctgg tgcggaccgg cttcggctcg aacaacgtcg atcactgcac
     3181 gcggctgtgc cacgcgtcgt cggtggcggc ccttatggaa ggggtcaact ccggtgccgt
     3241 gacagcacct ttcgccgccg cgctcgatgc cgaggtgatc atcgtcatcg gcgccaaccc
     3301 gaccgtgaat cacccggtcg ccgccacctt catcaagaac gccgtgaagg agcgcggggc
     3361 caagctgatc gtcattgatc cgcgtcgcca gatgctgtcg cggcacgcct tgcaccactt
     3421 ggcgttcaag ccgggcagcg acgtcgcgtt gctgaacgcc ctgctgcaca cgatcatcga
     3481 ggaggggctg accgacgacc agtatatcgc cggctacacc gagggctacg aggaactccg
     3541 cgaaaggata aaggaattct ccccggagcg gatggaaccc gtgtgcggca ttcccgcgca
     3601 gactctgcgt gaggtcgctc ggctttatgc ccgttcccga gcgtcgatca tcttctgggg
     3661 aatgggcatc agccagcacg tccacggcac cgacaacgca cgatgcctga tcgcgctggc
     3721 gctggtgacc ggccagatcg gcagaccagg caccggactg cacccgctgc gtggccaaaa
     3781 caacgtgcaa ggcgcctccg acgccgggct gatcccgatg gtgtttccgg actaccagtc
     3841 ggtcgagaag gtcaatattc gcgagcagtt cgagacgttc tggaagaccg aactggaccc
     3901 ggtgcgcggc ctgacggtcg tcgaaatcat gaacgcgatc cacggcggac agatcaaggg
     3961 catgtacatc gagggcgaaa accctgccat gtcggatccg gacctgaacc acgcgcgcgc
     4021 ggcactggcc aagcttgaac acctcgtcgt ccaggatctg ttcctgacag aaacggcatt
     4081 ccatgccgac gtcgtgctgc cggcttccgc cttcgcggaa aagagcggca cgttcaccaa
     4141 tacggaccga cgggtgcaaa ttgcccggcc agtgatcgac ccgcccggcg aagcgcggca
     4201 ggattggtgg atcatccagc aggtcgggca gcgcctgggc ttggactggg cgtatgccgg
     4261 gccgagcgat atttttgctg aaatggccca ggtcatgccg tccctcaaca acatcacctg
     4321 ggagcggctg gagcgcgagg gtgctgtgac ttacccggtg gatgcgccgg atcagccagg
     4381 ccacgaaatt atcttctact cgggtttccc gaccgagagc gggcgcgcga agatcgtgcc
     4441 tgcgaacgtg ttgccacctg acgaggtgcc ggacgacgac tacccgatgg tgctgtccac
     4501 cggccgggta ttggaacact ggcacacggg ttcgatgacc cggcgctccg gcgttctgga
     4561 cgcgatcgaa cccgaggcga tcgcattcat gtcgccgaag aatctctggc gggaaaacct
     4621 gcggccaggc gaccttattc ggattgaaac ccggcggggt gtcgtggagg tcaaggtacg
     4681 gtctgatcgc gacgtgccgg agggaatggt gttcatgccg ttctgctacg ccgaggccgc
     4741 gatcaacctg ctgaccaacc cggcgctcga tccgttcgga ctgatccccg agttcaaatt
     4801 ttgcgccgct cgggtgacgg catccgttga cagcggtggg atgatggcga agtagccgcc
     4861 ccggctgctc tgacggccgg tcccgcctcc ggcgcccgcg aagcgttgac catgggtcat
     4921 ggccgtgctt ggcgtggcca ccccacgact ttgctcaaac gcaggaatgt cgtcggcagc
     4981 caaccgttcc acaggaaccg gtaatggcta cccgtccggc cgggggacgc ccgcgatgca
     5041 atcacagacc gtgcctgaag cggttttggg ctggacttta atgggtcagc cggcgtggct
     5101 ggcgcttcca ggcggcacga gcgggcgaga acgtttccca cgcccgatca cgcgggtccg
     5161 cggcaaccag gatgtcgatc agcgagtccg gcaccacgac ctgctccgaa ggcagcctgt
     5221 cgagtgtgcc aatgcggttc atctgggcaa tgacgatccc gccgcacttg cgcgccgcgc
     5281 gagcaatcgc catggaatcg cgggtcatcg tgatggcggc ggtgccctcc atgaatgcca
     5341 cgccaattaa cgcggcatcg atcggaaatc cctggaacag caaaacgtca ttgccggcgg
     5401 gacgcaccag acgcaccagt tgctcccatg tgcgggggtt cagccggccg ccgccgttgc
     5461 gcgggtcggt gaatgtcccc aacccgctgc gcgaaaggtg acccggcatg ccctcggcga
     5521 tatcgcggaa cagccgcctg atgacgccga cgggaaaact gtaagcctcg atccggttgg
     5581 cggctgcgag cgcttgcaaa ccgaataccg gatgccactg accaccgacc acccgccgaa
     5641 ccaggccctc atgtgcgaga agattgaggc catggttgcg gccatctccc agggtagccc
     5701 catacacgat ggtcaggccg ttcggctgcg tgttgagggc gaaagcatcc gccaacgcgg
     5761 tcgcgaggct gtcgcccaac cagcccacgg cgaccgtggc acccggccgg attagccctg
     5821 ccgcctcctt cgccgaggcg ttcttccaat gccgctgtgg acgagcgtcg gatcccggcc
     5881 gagatgacaa tgacagcatc gac
//
LOCUS       DASWQN010000758         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1656955, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000758 DASWQN010000000
VERSION     DASWQN010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656955"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VGC82_12840"
     CDS             <1..1083
                     /locus_tag="VGC82_12840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0424200.1"
                     /translation="AVTRRLLAFAKRGNLQTEEVDPTVVLVDMQELLNHTLMGSIVVE
                     LDLPPWLPMILVDRMQLETVLVNIANNARDATPSGGMITISARQESVHENGMQAAGLT
                     AGSYVRLSVRDRGIGMDAKTLARAVEPFFSTKGPGQGTGLGLSMAKGFIEQSGGKLHI
                     VSRVGEGSTVTLWFPAMGTRSLRTAKGRIPTWTTGTSTPTTAPMPVALTQAEPRGSNT
                     TVLVVDDNRQTRELICEWLSECDFEVHQAASGEAALATMEGGLEIDMLLTDFSMPGMN
                     GIELAHAVRKRTPGQPVIILTGNTGEIAIDAIGKAVGGACTVLQKPIRISELPDHIKV
                     ALLRSPASQSSLALLVNLASAGGTPA"
     gene            complement(1400..1714)
                     /locus_tag="VGC82_12845"
                     /pseudo
     CDS             complement(1400..1714)
                     /locus_tag="VGC82_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1741..>1930)
                     /locus_tag="VGC82_12850"
     CDS             complement(1741..>1930)
                     /locus_tag="VGC82_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY0424201.1"
                     /translation="MNGRQMVDAARLARPGLKVLFITGYAENAVIGHGILEPGMHVLT
                     KPFALDDLAHRIRAIISS"
BASE COUNT          355 a          556 c          645 g          374 t
ORIGIN      
        1 gcggtgaccc ggcggttgct cgccttcgcg aagcgaggaa atttgcagac agaggaagtg
       61 gatccgacag ttgtacttgt tgatatgcag gaactgctca accataccct aatgggtagt
      121 attgtcgtcg agttggacct cccgccatgg ctgcccatga tcctggtcga tcggatgcag
      181 cttgagaccg tgctggtcaa cattgccaac aatgcgcgcg acgcaacacc gagtggcggc
      241 atgattacga ttagtgcccg tcaggaatcg gtacacgaga atgggatgca agcggctgga
      301 ctgactgccg gcagttacgt ccggctgagt gtgcgtgatc gcggcattgg catggatgcc
      361 aagacactcg cccgggcggt tgagccgttt ttctcgacaa aaggtcccgg ccagggaact
      421 ggtctcggtc tatcaatggc caagggcttc atcgagcagt cagggggaaa gctgcacatc
      481 gtgtcgcggg ttggtgaagg cagcacggtg acgctgtggt tcccggctat gggcacgaga
      541 tcgctgcgga ctgctaaggg gcggatcccc acatggacca ccggcacgtc gaccccaacg
      601 actgcgccca tgccggtcgc cctgacgcag gcagagccgc ggggcagcaa caccacggtt
      661 ttggtggtgg atgataaccg acagacgcgg gaactgattt gcgagtggtt aagcgaatgc
      721 gacttcgagg tccaccaagc tgcatcgggg gaggcggcgt tggcgaccat ggagggcgga
      781 ctggaaatcg acatgctgct gacggatttc tcgatgccgg gaatgaacgg gattgagttg
      841 gcgcacgccg tgcgcaagcg caccccgggg caacctgtga taatcctgac tggcaacacg
      901 ggtgaaattg cgattgatgc aattgggaag gcggtcggcg gcgcatgcac ggtgttgcag
      961 aagcccatcc gaattagcga attgcccgac catatcaagg tcgcccttct ccggtccccc
     1021 gcctcccaat cctctctggc gctgctggtc aatctggctt ccgcaggagg tacgccggct
     1081 taagcctcct tcctccatag gccagagttt gcgcctgccg cgggcggcgc tgaaggcgga
     1141 cgataggctg ggcaggctgt cgatagcgat ccacgggcgg cgaatgtggg gactatgcag
     1201 tgacagttcg cgcataggag ggccaactct tcgagcttag tcttttgtcg aaaccggctt
     1261 tgtatgatga caaatctcgc agccgcgctt acgcccccaa cctggaaatc gtactatgcg
     1321 agacgttgta ggtgcgggcg atatcgcatc ggctcgccgg cgtcgcgacg gcgcagggct
     1381 tccttgacct ggtgggctgt cagcttggcc ggccggccat tctcgcgccg cgggcttggg
     1441 cgcggaggcg gccttcgccg gtccgcgcgc ggatcagttc gcgctcaaac tccgccaacc
     1501 cgcccagcac cgtcagcatg aggcgaccgt gcggtgttgt ggtatcggcc tatgtgtcac
     1561 caagggaacg gaacctggcc ttgcggtcgg tgatcgtggc gagggtgttg agaagatccc
     1621 gcgtcgatcg cgccagccga tcgagccggg tgaccatcag cacgtcgtcc tcgtccagcc
     1681 tggcgtgggc cttgcgcagt tcggtgcgat ccacctgggc tccgcttgct aagggttcgg
     1741 tcagcttgaa ataatcgccc gtatccgatg cgcgaggtcg tcgagcgcga acggcttggt
     1801 caggacatgc atgccgggtt cgaggatgcc gtggccgatc accgcgttct cggcgtagcc
     1861 ggtgatgaac agcactttca gccccgggcg cgccaggcgg gccgcgtcaa ccatctggcg
     1921 gccgttcatg
//
LOCUS       DASWQN010000759         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_73719, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000759 DASWQN010000000
VERSION     DASWQN010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73719"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..956)
                     /locus_tag="VGC82_12855"
     CDS             complement(69..956)
                     /locus_tag="VGC82_12855"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0424202.1"
                     /translation="MRVGFIGVGNMGGPMCRNIIRNTNHEVVVFDLNPDAVSACTGLG
                     ASSGTSVADVASRCDVVITSLPIPRIVEDVALGAGGIAENARPGTVFIDLSTNSPATA
                     KRVNEGMKAKGIPMLEAPVSGGTARATDGTIVIMVGGDETIFEQNLPLLKSFSGEVIH
                     VGEIGYGSTAKLINNMLAFCNSAAAAEALMIGKRSGIDLRKLDAVIRNASGMSAGYAN
                     LSSKTLAGDFKPTFALDLAHKDLRLALEMADELGVPGMIAPQVMNLMRMARGMGLGSS
                     DSASLVRVYETTLGEDIRG"
     gene            complement(1109..2149)
                     /locus_tag="VGC82_12860"
     CDS             complement(1109..2149)
                     /locus_tag="VGC82_12860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0424203.1"
                     /translation="MPEHPERLDHFMARANAIYYATRDPFADFTTAPEISQVFGEIVG
                     LWAAVSWRLLGNPAPVALVEAGPGRGTLMTDAIRAIRRAAPDFADALSIHLIETSPRL
                     RAAQAQRLPDATWHDDMSTVPAQPIILLANEFLDALPIRQFVRRGSGWTERFVAERFG
                     AGRFVAGGQWIEQPANACEVPPKANVEGDLVEGDIVELNETARGFVGATAERLTHQPG
                     VALFLDYGSERSAAGDSLQALARKRPVDPLVHAGSADLTAHVDFADLAEVARARGAGV
                     QGPIAQGTFLTTLGLFQRTEKLAAGRPLAQVAELMKAARRLAEPAAMGQLFKAMAVHS
                     AGCPALPGFVPP"
     gene            complement(2217..>2431)
                     /locus_tag="VGC82_12865"
     CDS             complement(2217..>2431)
                     /locus_tag="VGC82_12865"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEY0424204.1"
                     /translation="GIFLCGYATARIIGEHFREPDAFLGFLPFGTTMGQLLSIPMFLA
                     GLWLIVRARPVDRQTADPVSATADRI"
BASE COUNT          410 a          760 c          827 g          434 t
ORIGIN      
        1 tttttgacgt tacacgccaa ccgtgggccc cggcaaccgg tgtggtgcaa ctttccgccg
       61 cagccaattc agccgcggat atcctcgcca agtgtcgttt catagacgcg aaccagtgag
      121 gccgagtcgg aggaacccag ccccatgccg cgggccatgc gcatcaggtt catgacctgc
      181 ggggcgatca tccccggcac gccgagttca tcggccattt ccagtgccag gcgcagatcc
      241 ttgtgcgcga ggtccagcgc gaaggtcggc ttgaagtccc cggccagcgt tttcgacgac
      301 agattggcat agccggcgga catgccgctg gcgttgcgga tcaccgcgtc cagtttcctc
      361 aggtcgatgc ccgagcgctt gccgatcatc agcgcctcgg ccgcggcggc ggagttgcag
      421 aacgccagca tgttgttgat cagcttggcg gtggagccgt agccgatttc gcccacatgg
      481 atgacctcgc cgctgaagga tttcaacaac ggcaggttct gctcgaagat cgtctcgtcg
      541 ccgccgacca tgatcacgat ggtcccatcg gttgcgcgcg cggtgccgcc ggagaccggg
      601 gcttcgagca tcgggatgcc cttggccttc atgccctcgt tcacccgctt cgcggtcgca
      661 ggggagttgg tggacaggtc gatgaacacc gtgcccggcc tggcgttctc ggcgatgccg
      721 ccggcgccga gggctacgtc ctcgacgatg cgggggatcg gcagggaggt gatgaccacg
      781 tcgcagcgcg acgcgacatc ggcgaccgag gttccggacg atgcgccgag gccggtgcag
      841 gccgacaccg cgtcgggatt caagtcgaag accacgactt catggttggt gttgcggatg
      901 atgttccggc acatcgggcc gcccatgttg cccacgccga taaagccaac tctcatgtct
      961 ggtttcccct tgtgccgctc gaacctatgg cgcccggaca ttgtggcgga agtgggaatg
     1021 gttgtcagga ggtctcggga ccggtgcgct cgcgctgctg cacgccatgt tctgccaggg
     1081 cgggatgttc ggttagggcg atgttcggtt agggcgggac gaaaccgggt agggcggggc
     1141 aacctgccga gtgcactgcc atggccttga acagctgccc catcgccgcc ggttctgcca
     1201 ggcggcgagc ggccttcatc agttcggcga cctgtgccag cggccggccg gcagccagtt
     1261 tctcggtgcg ctggaaaagc cccagtgttg tcaggaacgt tccttgcgcg atgggaccct
     1321 ggacgccggc accgcgcgcg cgcgctacct cggcgagatc ggcaaaatcg acatgcgcgg
     1381 tcagatcggc ggaaccggca tgcaccaacg gatcgaccgg acgcttgcgc gcaagtgcct
     1441 gcaggctgtc gcccgccgca ctgcgttcgg agccatagtc caggaaaagc gccacgcctg
     1501 gttggtgcgt caggcgttcg gccgtggcgc cgacgaagcc gcgggcagtc tcgttcaact
     1561 cgacgatgtc tccctccacg aggtctccct ccacgttggc cttgggcggc acttcacagg
     1621 cattggctgg ttgctcgatc cattgcccac ctgcgacgaa ccggcctgcg ccgaaccgtt
     1681 ctgcgacaaa ccgttcggtc cagccggagc cgcggcggac gaactggcgg atcggcaggg
     1741 cgtcgaggaa ctcgttggct agaagtatta tcggctgggc gggaaccgtg gacatatcgt
     1801 catgccaggt ggcgtccggt agccgctgcg cctgggcggc acgcaagcgg ggcgaagtct
     1861 cgatcaggtg tatgctcaac gcatcggcga agtccggtgc ggcgcggcgg atggccctga
     1921 tggcgtccgt catgagggtg cctcggccgg gtccggcttc caccaacgcg accggcgcgg
     1981 gattgccgag aagccgccag ctcaccgccg cccatagacc aacgatttcg ccgaagacct
     2041 ggctgatctc gggcgcggtg gtgaagtcgg caaatgggtc ccgggtcgca tagtagatcg
     2101 cgttggcccg agccatgaag tggtccaggc gttcagggtg ttcaggcatg gccgatcccg
     2161 ggacaggaaa tgtgcgacgc cggcatgatg atgatccgct agatgacgat gatccgctag
     2221 attctgtccg cggtcgcgct tactggatct gccgtctgcc gatcgaccgg ccgtgcccgc
     2281 acgattagcc acaggccggc aaggaacatc gggatcgaca gcaattgccc catggtggtg
     2341 ccgaacggca ggaaccccag aaaggcgtcg ggttcgcgga agtgttcgcc gatgatccgg
     2401 gccgtggcgt agccgcacag gaaaatgccg g
//
LOCUS       DASWQN010000760         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2053309, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000760 DASWQN010000000
VERSION     DASWQN010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053309"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VGC82_12870"
     CDS             complement(<1..592)
                     /locus_tag="VGC82_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0424205.1"
                     /translation="MSSTPQLQPVLDRIDADFDNSLERLFALLRIKSISADPAFAADC
                     KAAADHLARDIATLGFKAEVRPTAGHPAIVAKSNGKANGGARPQVLFYGHYDVQPVDP
                     LNLWHRPPFEPVVTDHADGRKIIVARGAEDDKGQLMTFIEACRAWKSVTGSLPVDITI
                     VIEGEEEIGSKNFVPFLETHKDELKADFALVCDTGMW"
     gene            complement(620..>1796)
                     /locus_tag="VGC82_12875"
     CDS             complement(620..>1796)
                     /locus_tag="VGC82_12875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY0424206.1"
                     /translation="LWFWAGQQVLQGRDLYPSDPKAYFDFIYPPLSAVLLAIPGYFGK
                     IPLYLCLSVFNAIAWWLTGQLSNAMTGAGRTPGPWLFALPGFVTISFVFDMFDLGQPN
                     LILLAMMLFGFWLLQHKRPWMAGGMFALATAIKVFPVAVLPYLVWRRRWTSVASTVVF
                     LAIFLFVVPAPVRGFQHNFSELKTWFQGMVGSSSEQGFGQRGQQNWSWVNQSIIAVTH
                     RLVRPVNYNQDNPAKPAAYMNLLDLDFKMANWVVLAVSFAIGLGFIAVMPPRARVTPR
                     SNAEELGILFCLMTVASPLARQYYFMWLFFPMTVLIHRAAYDPRRGVRVGTWATLAAA
                     GILMCLSLPVFPNDLQAYGNNLAAAAIIAGGLAWHLLHPPVEAAAGSLSPSALKPAE"
BASE COUNT          398 a          552 c          546 g          300 t
ORIGIN      
        1 cccacatgcc ggtgtcgcaa accagggcga agtcggcctt caactcgtct ttgtgggttt
       61 caagaaacgg aacgaaattc ttcgatccga tctcttcctc gccttcaatg acgatcgtga
      121 tgtcgaccgg caacgaaccg gtcacagatt tccaggcccg gcaggcttcg atgaacgtca
      181 tcaactggcc cttgtcgtcc tcggcgccgc gcgcgacgat gatcttccgt ccgtcggcgt
      241 gatcggtgac gacgggctcg aacggcggac ggtgccacag attgagcgga tcgaccggct
      301 gcacgtcgta atgaccgtag aacagcacct gcggccgcgc cccgccattt gccttgccgt
      361 tgcttttcgc gacgatggcg ggatgaccgg cggtcggccg gacctcggcc ttgaaaccga
      421 gcgtcgcgat atccctggcg agatgatcgg ctgccgcctt gcagtccgcg gcgaaggccg
      481 gatcggcgga gatcgacttg atccgcagca gggcaaacaa ccgctctagg ctgttgtcga
      541 aatcggcatc gatgcggtcg aggaccggct gaagttgcgg cgtgctggac attcgaaaat
      601 cttccctgag ttggcggggc tactcggcgg gttttagcgc cgacggagac aaggagccag
      661 cggctgcctc caccggcgga tgcaggaggt gccaggccag tccgccggca atgatggccg
      721 cggcggcaag gttattgcca taggcctgca ggtcattcgg aaacaccggc aacgacaggc
      781 acatcagaat cccggcggcc gcgagtgtcg cccacgtccc cacgcgtaca ccgcggcgtg
      841 gatcgtaggc cgcccgatga atcaggacgg tcatcggaaa gaacagccac atgaaataat
      901 actgccgggc cagcggggac gccaccgtca tcaaacagaa caggatgccg agctcctcgg
      961 cattcgagcg cggtgttacc cgcgcccgcg gcggcatcac cgcgatgaag ccgagcccga
     1021 tcgcgaatga caccgcgagc acgacccaat tcgccatctt gaaatcaaga tcgagcagat
     1081 tcatgtatgc cgccggcttg gccggattat cctggttgta attcaccgga cgcaccagac
     1141 ggtgcgtgac cgcgatgatg gattgattga cccaggacca gttctgctga ccgcgctgtc
     1201 caaacccctg ctccgagctt gagccgacca tgccctgaaa ccaggtcttc agttcggaga
     1261 aattatgctg aaaaccgcgt acgggcgctg gaaccacgaa cagaaaaatc gccaggaaaa
     1321 ccaccgtgct tgcgacggac gtccaccgcc ttcgccaaac cagataggga aggactgcta
     1381 ccggaaaaac cttgatggcg gtcgcaagcg caaacatgcc gcccgccatc cagggccgct
     1441 tgtgctggag cagccagaag ccgaacagca tcatcgcgag caggatgagg tttggctgcc
     1501 cgagatcgaa catgtcgaaa acaaaggata tggtgacaaa tcccggcagc gcgaacagcc
     1561 acgggccggg agttcgcccc gcgcccgtca tcgcgttcga gagctgaccc gtcagccacc
     1621 atgcgatcgc gttgaaaacc gacaggcaga gatagagcgg tatcttgcca aaatagccgg
     1681 gtattgcgag caacaccgcc gatagcggcg ggtagatgaa atcgaaatag gccttgggat
     1741 cgctcgggta gaggtcccta ccctgcagca cctgttgccc ggcccagaac cagagc
//
LOCUS       DASWQN010000761         2181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1524469, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000761 DASWQN010000000
VERSION     DASWQN010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524469"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VGC82_12880"
     CDS             <1..1024
                     /locus_tag="VGC82_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424207.1"
                     /translation="ISHYPPLAGPDDRMCTILRRMLQNPDLPPRLRTPDGWPEPMRAE
                     WGDDEGTTSAAHHRAALVGWMERTRSALDAFNPDFVLLWGDDQYENFREDIIPPYCIA
                     AYDKFAFSPPPNNVWGETDKTYEIAGNRKAAKMLASRLIEQGFDTAYAYKPLHHPLGH
                     AFTNAVFYLDYNRQGFPYPIIPFAINCYGRKVVSQRGGVPAFGKTDPDAEQDPPAPAP
                     WRLFDLGAATARILVESPYRVALLASSGWSHAFLTAKNHYLYPDTPSDRRMYEHLRDG
                     NYDAWRTYPANAVEDSGQQEILNWMCLTGALNALGRKPRETGFVDTWIFNSSKTFLIA
                     PPGPSG"
     gene            complement(1417..2007)
                     /locus_tag="VGC82_12885"
     CDS             complement(1417..2007)
                     /locus_tag="VGC82_12885"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0424208.1"
                     /translation="MNRRDLLFAGTATGLASLVPRSAAHAQVTHQFGESWFTNVTLQT
                     HDGRSVRFYDDLLKGKGAIINFMFTRCDGVCPGMTQNLAYVQELLGERVGRDIFMYSI
                     TIDPEHDSPEMLARYAAPFDIRPGWLFLTGAKKDVELLRARLGFVDSDPREDADPDEH
                     TGTVRIGNEPLHRWVMAPGLANPQAIVRTVLRVLPA"
BASE COUNT          446 a          687 c          652 g          396 t
ORIGIN      
        1 gatatcccac tacccgccgc ttgccggtcc cgatgaccgc atgtgcacga tactgcgacg
       61 catgctgcag aaccccgacc tgccgccgcg gttgcgaacg ccggacggct ggccggagcc
      121 gatgcgggca gagtggggcg acgacgaggg taccacatcg gcagcgcacc atcgtgccgc
      181 cttggtaggc tggatggaac ggacccgaag cgccctcgac gcattcaatc cggatttcgt
      241 tttgctatgg ggcgacgatc agtacgagaa ctttcgcgag gacatcatcc cgccttactg
      301 catcgccgct tacgacaaat tcgccttctc gccgccgccg aacaacgttt ggggcgagac
      361 ggacaagacc tacgagatcg ccggcaatcg caaggccgcg aaaatgctgg caagccgcct
      421 gatcgagcaa ggtttcgaca cggcctatgc ctataagcca ttgcatcacc cgcttggaca
      481 cgccttcacc aatgcggtct tctaccttga ttacaaccgc caggggtttc cctatccgat
      541 cataccattc gcgataaact gctacggacg caaagtggtg tcgcagcgtg gcggcgtccc
      601 tgccttcggc aagaccgatc cggacgccga acaggacccg ccggcacccg caccgtggcg
      661 actgttcgac ctcggcgccg cgaccgctcg catcttggtc gagtcgccat atcgggttgc
      721 tctgctggcc tcctccggct ggtcgcacgc tttcctgacc gccaagaacc attaccttta
      781 tccggacacg ccttcggatc ggcggatgta tgagcacctt cgcgatggca actatgacgc
      841 atggcgaacc taccccgcga acgccgtcga ggacagcgga cagcaggaaa tcctgaactg
      901 gatgtgcctg acaggcgcat tgaacgccct cgggcgcaag ccgcgggaga cgggcttcgt
      961 cgatacctgg attttcaact cctcgaagac gttcctgatc gcgcctccag gcccgtccgg
     1021 ataagaccct gctcggcgga gatcagcctc ggtcacccct ggtcttgatg cccgccgccg
     1081 cggcagcgtc cgccagtcgg aaaggatggt atccggcact ccggcctacg atgtgatcgt
     1141 tgctggagcg agccggcgcg gcggccgact gaatggacga aagggcctcc tgatccaggt
     1201 cggctgaaca gggctgaaat actcgccaaa actttgggta tccgacgagc gcgccaaccg
     1261 ctgagcgcca cgaccataga aaagaagtct atcgagacca catatatttg accaaagtct
     1321 ttccgaccca caacgattcc aaatcggatg tggttgaatc agcatcggaa cgaacgcatt
     1381 gacgtcatcc tgcacgcccg taggacgagc gggaagctac gccgggagca cccgaagcac
     1441 ggttcgcacg atggcctggg gattggcgag gccgggcgcc atgacccagc gatgaagcgg
     1501 ctcattgccg atccttacgg tgccggtatg ctcatcggga tcggcatcct cgcgcggatc
     1561 agagtcgaca aaccccagtc gcgcgcggag caactccacg tccttcttcg cgccggtcag
     1621 gaacagccag cccggacgga tatcaaatgg ggctgcgtat cgggccagca tctccggcga
     1681 gtcgtgctcc ggatcgatgg tgatcgagta catgaagatg tcacgaccga cccgctcccc
     1741 gagcagttcc tgaacgtagg caaggttctg tgtcatgccc gggcagactc cgtcgcaccg
     1801 agtgaacatg aagttgatga tggcgccttt ccccttcagc aggtcgtcgt aaaatcgcac
     1861 gcttcggccg tcatgtgtct gcagcgtgac gttggtgaac caggattcgc cgaattggtg
     1921 ggtcacttgc gcatgcgcgg ccgatcgcgg gaccagcgaa gcgaggccgg tcgcggtgcc
     1981 tgcgaagagt aggtcgcgtc tgttcatgag gactccggtt tgatttccaa ggtgaccgcc
     2041 gctcggtcac cgctgggcaa cccaccagcg caagcggcgg aacgttgtcg cggccgatca
     2101 tgccttgggt taatgattat gtccatacca tgggtgtaac ggcagattgc agacgcagac
     2161 agtgcggcac cgaacgaaac a
//
LOCUS       DASWQN010000762         5530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1789462, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000762 DASWQN010000000
VERSION     DASWQN010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789462"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2326)
                     /gene="lon"
                     /locus_tag="VGC82_12890"
     CDS             complement(<1..2326)
                     /gene="lon"
                     /locus_tag="VGC82_12890"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY0424209.1"
                     /translation="MDTQPATLPDQTGDDVLLVPVREVVLFPGVVLPLLANRPGLEAA
                     LQEAARNSRTIAVVLQRDPTTEAPGLSDMYPIGTEGRLLRYVTGRDGMHHGIVQGVGR
                     IRLLDIVDGTDPRTVRVERIPEPTDGGTDIDARFHQLRERALETLGLLEQSPPEIAAA
                     VQSIEQPGQLADMVASLMDLAPGEKQEILETVPLLARLDRVLWRLAYRLEVLRLSADI
                     GKRTRATMEGRQREFMLREQLKAIQKELGEDEGGSPELADLRRTLDEAGLPEEVNKQA
                     QRELRRLERMPDGSPESGMVRTYLEWLSELPWKIGDDRAIDIAEARRVLDQDHHALEK
                     IKRRIVEFLAVRKLNPDGRGPILCFVGPPGVGKTSLGQSIARAIGRPFVRVSLGGVHD
                     ESEIRGHRRTYIGALPGNIIQAIRRAGSRACVMMLDEIDKLGAGGFQGDPSSALLEVL
                     DPEQNATFRDAYLAVPFDLSRVLFIATANQMDTIPAPLRDRMEVVSLSGYTEEEKLVI
                     ARRYLVPRQLGRNGLKPEQAEIDDTALSAIVRDYTREAGVRNLEREIGAVLRNVAAQF
                     AEGHADKMRIGPGDLPAILGPSRFEAEVANRVAMPGIATGLAWTPVGGDILFIESTRM
                     SGNGKLILTGQLGDVMKESAQAALSLLKSRASSLAIEPGLFERSDIHVHVPAGAIPKD
                     GPSAGVAMFMSLASLMVGRTVNPAIAMTGEISLRGIVLPVGGIREKVVAAERAGITTV
                     MLPARNRRDYDDIPASTRNRLKFVWLETVDDAMAT"
     gene            complement(2326..2715)
                     /locus_tag="VGC82_12895"
     CDS             complement(2326..2715)
                     /locus_tag="VGC82_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY0424210.1"
                     /translation="MPRSASDIMWAEAVAALARVDRLHRDFFRPDPKGWEPPVDVLET
                     QGELVIMVALPGVRGDEVEIVVHDDSLAIVGVRRLPAFLQRARVHRMELPHGRFERRV
                     AIPSGRYELTRRDLADGLLVVALRRFG"
     gene            complement(2976..5033)
                     /locus_tag="VGC82_12900"
     CDS             complement(2976..5033)
                     /locus_tag="VGC82_12900"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY0424211.1"
                     /translation="MNLRIGIDIGGTFTDLVAISHDGTVTTHKTASTPHDYGEGIIEG
                     LRALLADRPGWVGDVLHATTVGSNTVLEAKGAKTALITTRGFRDILEIRDLRMPVLYD
                     IGWTKPRALVERRLRLEVTEKMRPDGTVAVPLDPASLGPVLAMLRSENVVSVAICLLH
                     SYANPAHERAVAHAVREALPDIAISVSSEILPEIKEYPRTSTTVINAYVQPVVRAYIT
                     ALDARLRGLGIEAPLQLMQSNGGLASASFAADWPAHIIESGPAAGVVGGAALARRLDE
                     PRIVTFDMGGTTAKAGLVENGKVLRTEAIEIGGGVMAGSRLLVGAGYMLKLPAIDLAE
                     VGAGGGSICRLDSAGAPKVGPESAGAIPGPVCYGRGGIAPTIADCTLALGYLDTGGLT
                     GGTVRLDLEAARAAILRDLAEPLGLSVEAAAHGMLRLASATMMRAIRAVSVERGRDPR
                     QFAMLAFGGNGPLFAAGIAAELGIGRVIIPPLPGLFSAFGLLVADAEHHARQSIRVRL
                     GEADPDRISRIITDLAEAGAQRLGRDGFASERQVFQRGAFARYVGQSSEIEVALPDGA
                     VTPGLIAALFAEEHEKTYGFRAPEGEPVELISLSVMARGLPEQPRLPERIPPFTQAVP
                     THRRTWFPKEGWVETPVSDRAGLTGTVRGPFIVQEYDATCLVPRGATALVDDFGNIRL
                     ALG"
     gene            complement(5054..>5530)
                     /locus_tag="VGC82_12905"
     CDS             complement(5054..>5530)
                     /locus_tag="VGC82_12905"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY0424212.1"
                     /translation="RFRGGMSQRKTWRMLADEGILQVRADRQTHRPYGLQGGGPGEPG
                     LNVMNPGLPTETKLHAKLTMTLTRGEIFRHELPGAGGWGPALDRELDMVARDLRDGLV
                     SIKAAARDYGVVAAGDPPVIDPAATEVLRARLRAERKPLPSVAWERAVPIAWERAV"
BASE COUNT          948 a         1929 c         1703 g          950 t
ORIGIN      
        1 cagtcgccat ggcgtcgtcc accgtttcca gccacacgaa tttcagccga tttcgcgtgc
       61 ttgcggggat gtcgtcgtaa tcgcgcctgt tgcgcgccgg cagcatcacg gtggtgatgc
      121 cggctcgttc ggccgccacc accttttccc gaatgccacc cactggaagc acgatgccgc
      181 gcaggctgat ttcgccggtc atggcgatcg ccggattgac ggtgcggccc accatcaacg
      241 acgccagtga catgaacatg gccacgccgg ccgacgggcc atccttcgga atcgctcccg
      301 ccggcacatg aacgtggata tcgctgcgct cgaacagccc gggctcgatc gccaggctgc
      361 tggcacgcga tttcaacaga ctaagcgctg cctgcgcact ctctttcatc acatcgccta
      421 actgcccggt caggatgagc ttgccattgc cggacatgcg cgtgctttcg atgaacagga
      481 tatccccgcc gaccggcgtc caggccagcc cggtggcgat cccgggcatg gcgacccggt
      541 tggcgacctc ggcttcaaag cggctggggc cgaggatcgc cggcaggtcg ccgggaccga
      601 tacgcatctt atcggcatgg ccctcggcaa actgagcggc cacgtttcgc agcaccgccc
      661 cgatctcgcg ttccaggttg cggacaccgg cttcccgtgt gtagtcgcgt acgatcgcgg
      721 acagcgccgt gtcgtcgatt tcggcctgct ccggcttcag tccattgcgc ccgagctgac
      781 gcggaacgag gtaacgccgg gcgatcacga gcttttcttc ttcggtgtag ccggacaggg
      841 acaccacttc catgcggtcg cgcagcggcg cagggatggt gtccatctgg ttggcggtgg
      901 cgatgaacag gacgcggctc agatcgaacg ggacggctag gtaggcgtcg cggaaggtgg
      961 cattctgttc gggatccagc acttccagca gcgccgatga cggatcgcct tggaagcctc
     1021 cagcgcccag cttgtcgatt tcatccagca tcatcacgca agcgcgtgac ccagcacgcc
     1081 ggatggcctg gatgatgttg ccgggcaacg cgccgatata cgtccggcga tggccgcgga
     1141 tctcgctttc atcatgcacg ccgcccaggc tgacgcgcac gaacggccgt ccgatggcgc
     1201 gcgcgatcga ttggcccaac gatgtcttgc cgacgccggg tggcccgacg aagcacagga
     1261 tcggcccgcg tccatccggg ttcagctttc gcaccgccaa gaattccacg atgcggcgct
     1321 tgatcttttc cagcgcatga tggtcctgat cgagcacgcg gcgcgcctcg gcgatgtcga
     1381 tcgcacgatc gtcgccgatc ttccacggca gttcggacag ccactccagg tatgtgcgaa
     1441 ccatgccgga ttccgggctg ccgtccggca tccgctccag ccgccgcaat tcgcgctgcg
     1501 cctgcttgtt gacctcttcc ggcagtcccg cttcatccag tgtgcgccgc agatcggcca
     1561 gttccggtga gccgccctcg tcctccccca gctctttctg gatggccttg agctgctcac
     1621 gcagcatgaa ctctcgctgg cgcccctcca tggttgcgcg ggttcgcttg ccgatgtcgg
     1681 ccgacaggcg cagcacctcc aaccgatagg ccagccgcca caggacgcgg tcgagcctag
     1741 ccagcagcgg gaccgtttcc aggatctcct gcttttcgcc aggggcgagg tccatcaggc
     1801 tggcgaccat gtcggcgagc tggcccggct gttcgatcga ctgcacagcc gccgcgattt
     1861 cgggcggcga ttgctccagc aaacccagcg tttccagcgc ccgctcgcgc aactggtgaa
     1921 agcgggcatc gatgtcggtc ccgccgtccg tcggttcggg gatgcgttcg acgcgaaccg
     1981 ttcgcggatc cgtgccatcg acgatgtcca gcaatcggat ccgaccgaca ccctgaacaa
     2041 tgccgtggtg cataccgtcg cggccggtga cgtagcgaag cagcctgcct tcggttccga
     2101 tcgggtacat gtcggaaaga ccgggcgcct cggtcgtcgg gtcgcgctgc aacaccacgg
     2161 cgatggtgcg cgaattacgt gcggcttctt gcagcgctgc ctccagccct ggccggttgg
     2221 caagcagcgg caggaccacg ccggggaaca gcacgacctc ccgcaccggc accagcagaa
     2281 cgtcgtcccc ggtttgatct ggcaacgtgg ctggttgtgt atccattacc cgaacctccg
     2341 cagcgcaacg acaagcagcc cgtccgcgag atcccgccgt gtcagctcat agcggccgga
     2401 cggaatggcg actcgtcgct cgaatcgccc atgcggcagt tccatccgat gtacacgggc
     2461 ccgctgcagg aaggcgggaa gacgtcgtac gccgacaata gccaggctat cgtcgtgcac
     2521 cacgatctcc acctcgtccc cccgaacgcc aggcagcgcg accatgatga cgagctcgcc
     2581 ctgagtctcg agcacatcga cgggcggctc ccatcccttt gggtcaggac ggaaaaaatc
     2641 gcgatgcagg cgatcgacgc gggcaagcgc ggcgacggcc tccgcccaca tgatgtcgga
     2701 ggcgctacgt ggcatgttgg ccctcgaaac agttcttcat acaacgccca ggtcgaatgg
     2761 cgggttgccc cgcgaggtcc tgattccgct cgcggccagg ttccgagcgt ggctggccgg
     2821 gcggattgtg cccgtgactt cgctggtttg gtccaggcgc aagtatgacc gcgccgggca
     2881 gtacgcccat atcccgcgaa tcatgcgact atatcgcgtt acaggcaaat cttcgccctg
     2941 catgatcgac accttcatga tcggccaaaa atggcctatc cgagcgccaa ccggatattg
     3001 ccgaagtcgt ccacaagcgc ggtcgcgccc ctcggcacca aacacgtcgc gtcgtattcc
     3061 tgcacgatga acggaccacg gaccgtcccg gtcagcccgg cacgatcgct caccggtgtc
     3121 tccacccatc cctccttcgg gaaccaggtg cggcgatgtg tcggcacagc ttgcgtgaac
     3181 ggcggaatcc gctcgggcaa ccgaggctgc tccggcagtc cacgtgccat gacggacagg
     3241 ctgatcagtt cgaccggttc gccctccgga gcccgaaagc cataggtctt ctcgtgttcc
     3301 tcggcaaaca acgcagcgat cagccctggc gtgacggccc catcgggcaa cgcaacctcg
     3361 atctcgctcg actggccgac gtaacgggcg aacgcgccgc gctggaaaac ctgtcgttcg
     3421 gaggcaaagc catcgcggcc tagtcgttgc gccccagcct ccgccaaatc ggtgatgatc
     3481 cggctgatgc ggtccggatc ggcttccccc agccgcacgc ggatgctctg gcgcgcatgg
     3541 tgttcggcgt cggcgaccag cagaccgaac gcgctgaaca gtcccggcag cggcgggatg
     3601 atgaccctgc cgatccccaa ttcggccgct attccggcag cgaacagcgg gccgttgccg
     3661 ccgaacgcca gcatggcgaa ttgccgcggg tcgcggccgc gttccaccga taccgccctg
     3721 atcgcacgca tcatggtggc cgacgccaag cgcaacatgc catgcgccgc cgcttcgacc
     3781 gacagcccca agggttcggc gagatcgcgc aggatcgcgg cccgagcagc ttccagatcc
     3841 agccgcacgg tacctccggt cagtccgccg gtatcgaggt agccgagcgc caaggtgcaa
     3901 tcagcgatgg taggcgcgat gccgcctcgg ccatagcaca ccggaccagg aatcgccccg
     3961 gcgctttccg gaccgacttt cggcgcgccc gcgctgtcca gccggcagat cgatccgccc
     4021 ccggcaccca cctcggccag gtcgatcgcc ggcagtttca acatgtagcc ggcgccgacc
     4081 agcagccgcg atccggccat gaccccgccg ccaatctcga tcgcctctgt gcggaggact
     4141 ttgccgttct caaccaaacc cgcctttgcc gtcgtcccgc ccatgtcgaa cgtgacgatg
     4201 cgtggctcgt ccaggcgacg agccagtgcg gccccaccga cgacgccggc agcgggaccg
     4261 gattcgatga tgtgggcggg ccaatccgcc gcgaaggaag ccgatgccag gccgccgttc
     4321 gactgcatca gttgcagcgg cgcttcgatc ccgagcccgc gcaaccgagc atccaaggcc
     4381 gtgatatagg ctcggacgac cggctggaca taagcgttga tcacggtcgt gctggtgcgc
     4441 ggatactcct tgatctccgg caggatttcg ctgctgaccg aaatggcgat gtccggcagg
     4501 gcctcccgga ccgcgtgcgc caccgcccgc tcgtgcgccg gattggcata gctgtgcagc
     4561 aggcatatcg cgacgctgac gacattctcc gaacgcagca tcgccagcac cggcccaagg
     4621 ctggccgggt ccagcggaac cgcgaccgtc ccgtcgggac gcatcttttc cgtaacttcc
     4681 aggcgcaggc ggcgttcgac caaagcacgc ggcttggtcc agccgatgtc gtacagcacc
     4741 ggcatgcgca aatcccggat ctccaggatg tcgcggaaac cacgcgtggt gatcaatgcc
     4801 gtcttggcgc ccttggcttc cagcaccgtg ttcgatccga cggtggtggc atgcagcacg
     4861 tcgcccaccc atcccggccg gtcggcgagc agcgcacgca gaccctcgat aatcccctcc
     4921 ccgtagtcgt gcggcgttga cgcggtcttg tgtgtcgtga ctgtcccgtc atgagaaatt
     4981 gccaccagat cggtgaacgt gccgccgatg tcgatgccga tgcgcaggtt caagccgtgt
     5041 gctcccacgc cactcatacc gcgcgttccc acgcgattgg taccgcgcgt tcccacgcga
     5101 cagaaggcaa aggttttcgt tcggcgcgca aacgggcgcg caagacttcg gtggcagccg
     5161 ggtcgatgac cggcgggtcg ccggcggcga ccacaccata atcacgggcg gcagccttga
     5221 tggaaaccaa gccgtcgcgg aggtcgcgtg ccaccatgtc cagctcacgg tccagcgccg
     5281 gaccccagcc tccggcaccg ggaagttcat ggcggaaaat ctcgccgcgc gtcagggtca
     5341 tcgtcagctt ggcgtgcagt ttcgtctcgg tcggcaggcc cgggttcatc acgttcagac
     5401 ccggctcgcc cggtccgccg ccttgcaacc cgtacggccg atgagtctga cggtccgcgc
     5461 gaacctgcag gataccctca tcggccagca tccgccacgt cttgcgctgc gacatcccgc
     5521 ccctgaaccg
//
LOCUS       DASWQN010000763         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_732497, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000763 DASWQN010000000
VERSION     DASWQN010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732497"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1671)
                     /locus_tag="VGC82_12910"
     CDS             complement(4..1671)
                     /locus_tag="VGC82_12910"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0424213.1"
                     /translation="MSQATSQATETPPAKMAGKVDVVIVGAGFGGMYMLHRVRGLGLS
                     AVVFDVASGVGGTWYWNRYPGARCDVESMQYSYSFSEELQQEWQWSEVFAGQPEILRY
                     ANHVADRFDLRRDMRFETRVTDAVFDEATHRWMVRTDRGDVVSAKYCVMATGCLSAAR
                     MPDFPGLDSFKGQTYHTGHWPHEGVDFAGKRVGVVGTGSSAIQSIPVIAQHAAHVTVF
                     QRTPNFSIPSRNRPMPEDYSRSWKDIYAAKRAEARLSRNGVLTNPNDQSAMDTPEQDR
                     LAVYENRWESGGTTFMAAFNDLIFNKASNDTAAEFVRGKIRDMVKDPAKAALLAPTNH
                     PIGTKRICVDTDYYLTYNQPNVDLVDVRNAPIEAVTPDGLLVGGKQYGFDAIVFATGF
                     DAMTGALTRMGIVGRGGRKLADKWEAGPRTYLGLMTADFPNMFMITGPGSPSVLSNMM
                     VSIEQHVDWVADCLGYLNSRQLDCIEPTLSAENAWVEHVNEVAHITLYPSAASWYMGA
                     NIPGKPRVFMPYIGGVGAYRQKCDEIAANAYQGFSVAGPANVAVAAE"
     gene            1901..2368
                     /gene="rplM"
                     /locus_tag="VGC82_12915"
     CDS             1901..2368
                     /gene="rplM"
                     /locus_tag="VGC82_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY0424214.1"
                     /translation="MLKSTPSIKPSEVTKGWILIDAEGVVLGRLAVIIASRLRGKHKP
                     QFTPHVDCGDNVVVINAAKVRVTGDKAEQSVFYYHTGYPGGIKGRTIRQRLESRHPER
                     VLEKAVERMITRGPLQRKQMKHLHVYGGGEHPHVGQQPATLDVAAMNPKNKRG"
     gene            2373..2909
                     /gene="rpsI"
                     /locus_tag="VGC82_12920"
     CDS             2373..2909
                     /gene="rpsI"
                     /locus_tag="VGC82_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY0424215.1"
                     /translation="MSGNSTGTLADLKDALSARQAAAEADTDASGVPAPLLASTQPHR
                     DPKRDELGRSYATGRRKESTARVWIKPGKGDIQVNGKRVVQYFARPVLRMLLTQPFLV
                     ADRYNQFDVFCTVSGGGLSGQAGAVRHGISRALTYYEPDLRSILKVAGFLTRDSRAVE
                     RKKYGKAKARRSFQFSKR"
BASE COUNT          605 a         1027 c          982 g          583 t
ORIGIN      
        1 ttgctattcc gctgcaaccg cgacgttggc aggaccggct accgagaaac cttggtatgc
       61 gttcgccgcg atctcgtcgc atttctggcg gtatgcgccg acgccgccga tataaggcat
      121 gaacactcgc ggcttgcccg gaatattggc gcccatgtac cacgatgccg cggacgggta
      181 gagcgtgatg tgggcgactt cgtttacgtg ctccacccag gcattttccg cggaaagggt
      241 gggttcgatg cagtccaact gccggctgtt cagataaccg aggcaatcgg caacccagtc
      301 cacatgctgc tcgatcgaca ccatcatgtt gctgagcacc gacgggctgc ccgggccagt
      361 gatcatgaac atgttcggga aatccgcggt catcaggccc agatacgtcc tcgggccagc
      421 ttcccacttg tccgccagct tgcgtccgcc acggccgacg atgcccatcc gggtcagcgc
      481 gccggtcatg gcatcgaagc cggtggcgaa gacgatcgcg tcgaagccat attgcttgcc
      541 gccaaccagc aatccgtccg gtgtcactgc ctcgatcggc gcattccgca catcgaccag
      601 atcgacgttc ggctggttgt aggtcaggta gtagtcggta tcgacgcaga tccgcttcgt
      661 cccgatcggg tggttggtcg gcgccagcag cgcggccttc gccggatcct tgaccatatc
      721 ccggatcttg ccgcggacga actccgctgc ggtgtcgttc gaggctttgt tgaagatcag
      781 gtcgttgaac gccgccatga acgtcgtccc gccgctttcc cagcggttct cgtacaccgc
      841 cagacggtct tgctccggcg tgtccatggc ggattgatcg ttggggttgg tcaggacgcc
      901 gttccggctc aaccgagcct cggcgcgctt cgcggcatag atgtctttcc aggaccgcga
      961 atagtcctcc ggcatcggcc ggttgcgcga cggaatgctg aagttcggcg tccgctggaa
     1021 taccgtgacg tgcgcggcgt gttgcgcgat caccgggatc gactggatcg ccgaggaccc
     1081 tgtcccgacc accccaaccc gctttccggc gaaatccacg ccctcatgcg gccagtggcc
     1141 ggtgtggtat gtctgcccct tgaagctgtc caggccggga aaatccggca tcctggcggc
     1201 cgacagacag ccggtcgcca tgacgcagta tttcgccgag acgacatcgc cccggtcggt
     1261 gcgcaccatc cagcggtgcg ttgcttcatc gaacaccgcg tccgtcacgc gcgtttcgaa
     1321 ccgcatgtcg cggcgcagat cgaaccggtc cgccacatgg ttggcatagc gcaggatttc
     1381 gggttggccg gcgaaaacct cgctccactg ccattcctgc tgcagttcct cggagaacga
     1441 gtaggaatac tgcatgctct cgacatcgca gcgggcgccc gggtagcggt tccagtacca
     1501 ggtgccgcca acgccggaag cgacatcgaa cacgaccgcg gacagtccca gaccacggac
     1561 gcgatgcagc atgtacatgc cgccgaagcc cgcgccgacg atgactacat cgaccttacc
     1621 ggccatcttc gcgggtgggg tttcggtcgc ctgggacgtc gcctgggaca tggatgctcc
     1681 ttaatatggt atgcaggcat ccgataccgt tccgccaaaa attgtcaacc agcgagtagg
     1741 ctttcacacc ggctggttca ggcgagcccg gcgaggcagc catgtccggt taccatgtct
     1801 ggtagccgcg tctgattgcc atgtctggaa accccttgac catacagggt tacgccgcca
     1861 taacccgccc cttctgaatt atcggacgct tgtaacaacc atgctcaaga gcacgccctc
     1921 catcaaacca tcggaggtga caaagggctg gatactgatc gatgccgaag gcgtcgtcct
     1981 cggtcgcctc gccgtcatca tcgcaagccg cctgcgcggc aagcacaaac cgcaattcac
     2041 cccgcatgtg gattgcggcg acaacgtcgt cgtgatcaac gcggccaagg tccgcgtcac
     2101 tggcgacaag gcggaacagt cggtcttcta ctaccacacc ggctatcccg gcggcattaa
     2161 aggccgcacc atccgtcagc ggctcgagtc caggcatccc gagcgcgtgc tggaaaaggc
     2221 ggtggagcgc atgatcacgc gcggcccgct gcagcgcaag cagatgaagc acctgcacgt
     2281 ttatggcggc ggcgagcatc cgcatgttgg gcagcagccg gcgacgctgg acgtcgcggc
     2341 gatgaacccg aagaacaaga ggggctgatc agatgtcagg aaactcaacc ggcaccttgg
     2401 ccgacctcaa agatgcactc tcggcgcgtc aggctgctgc cgaagccgat accgacgctt
     2461 ccggcgtgcc tgcgccgttg ctcgccagca cccagccgca ccgcgaccca aagcgcgacg
     2521 aactcggccg ctcctacgcc accggacgtc gcaaggaatc caccgctcgg gtttggatca
     2581 agcccggcaa gggcgacatc caggtgaacg gcaagcgggt ggtgcagtat ttcgctcgcc
     2641 cggtgctgag gatgctgctg acccagccct tcctggtggc agaccggtac aaccagttcg
     2701 acgtattctg cacggtcagt gggggcggac tatcgggtca ggccggcgcc gtccgccatg
     2761 gcatcagccg ggcattgacg tactatgagc cggatttgcg gagtatcctg aaggtcgcgg
     2821 gattcctgac ccgcgattcg cgcgcggtgg aacgcaagaa gtacggcaag gcgaaggccc
     2881 gccggagctt ccagttcagc aagcgctgat ccgccagaac cgacaaaatt agaaccgacg
     2941 aaaaggggcg cttacgggcg ccctttttcg caactcgggg atggcgagtc ggtgcgtttc
     3001 gtgcggacgc ggccgcgact ttgcgaacgt cctgcgaagg aagccggtgc ggatgaaagc
     3061 catggtggcc cgccacctgt cgccgcaggc ggcgtgttgc agacacgtga tggcgaagcg
     3121 tccttgccga agcgtttgct caagatcgct tcggacgcga ctaataccaa cggccgctga
     3181 ctccgacctt tgcgcga
//
LOCUS       DASWQN010000764         2777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_469543, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000764 DASWQN010000000
VERSION     DASWQN010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469543"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VGC82_12925"
     CDS             <1..861
                     /locus_tag="VGC82_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY0424216.1"
                     /translation="WLPGYQGASPVRIGNAASSQLQLDIFGELIDAMYQEAALGLVRP
                     AASWDLQCTLVSHLEKIWALPDESIWEVRGGAQNFTFSKVMVWVAIDRAIRGAEAFGL
                     PAPLERWKTLRTHIHETICREAYNEERKTFVQYFGGDTLDASLLLLPLVGFLPPEDER
                     IKGTVEAIGRDLMFDGLVRRYRTGETKDGLSGSEGVFIACSFWYVDNLVLLGRDQEAR
                     DMFDRLLGLANDVGLLAEEYDPVEQRQLGNFPQAFSHLALINTALNLGAAGGPAAQRS
                     CSANETPHGP"
     gene            complement(971..1273)
                     /locus_tag="VGC82_12930"
     CDS             complement(971..1273)
                     /locus_tag="VGC82_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424217.1"
                     /translation="MQLPGTTVKIPTGLAFEIPDLLMLQGWADFHELRMAIDLDVCVE
                     GDEYEELLGLYDKDRAFRRWMVWRSCDGIVVQPTMGRAMLFDYMADALETLIPARD"
     gene            1634..1798
                     /locus_tag="VGC82_12935"
     CDS             1634..1798
                     /locus_tag="VGC82_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424218.1"
                     /translation="MQKLTSTAQYAVRPQARAVATARDENSLLRGLTYGVVLSAAVWI
                     VAGYVTFALR"
     gene            complement(2006..>2777)
                     /locus_tag="VGC82_12940"
     CDS             complement(2006..>2777)
                     /locus_tag="VGC82_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0424219.1"
                     /translation="LSSVARFERATAPLAVRHQGQFPAATISFNLAPGVALSTAETAV
                     RDSTAELRMPEDVRTEFAGNARDMQQSLSTQPLLIAAALISIYIVLGVLYESLLHPLT
                     IISTLPSAGLGALLALWITGTDLSVMGMIGIILLMGIVKKNAIMMVDFALEAERQRGM
                     SPFDAIYAACLERFRPITMTTLAALLGALPLALAFGTGSELRRPLGIAIVGGLLVSQM
                     LTLYTTPVVYLALESLTRRRRTIVPVPPSDRAAPIGSP"
BASE COUNT          589 a          805 c          819 g          564 t
ORIGIN      
        1 tggcttcccg gctatcaagg cgccagcccg gtgcgtatcg gcaatgccgc gagttctcag
       61 cttcagctcg acatcttcgg cgaacttatc gatgccatgt accaggaagc ggccctgggt
      121 ctggttcgcc cggccgcgag ctgggacctg caatgtaccc tggtaagcca tttggagaag
      181 atttgggcgc taccggatga aagcatttgg gaagtccgcg gtggggcgca gaacttcacg
      241 ttctcgaagg tcatggtgtg ggtagcgatc gaccgggcca tccgaggtgc cgaggccttc
      301 ggcttgccgg cgccgctgga acgctggaag acgctgcgga cgcatatcca tgagacgatc
      361 tgccgggaag cctataacga ggaacgcaag actttcgtgc agtattttgg tggcgacacc
      421 ctcgatgcca gcctgttgct gctgccgctc gtgggttttc tgccgcccga agacgagcgg
      481 ataaagggta ccgtggaggc aatcggccgg gacctgatgt ttgacggcct ggtgcgccgc
      541 tatcgcaccg gggaaaccaa ggatggcctg tcgggcagtg agggcgtctt catagcctgc
      601 agtttttggt atgtcgacaa cctggtgctg ttaggccgcg atcaagaggc tcgagacatg
      661 ttcgacagat tgttaggcct ggcgaacgat gttgggcttc tggccgagga atatgacccg
      721 gttgagcagc ggcaacttgg caacttcccg caggcgtttt cgcatttggc gctgataaat
      781 acggcactta atctgggtgc cgcgggcggt ccggcagccc aacgcagctg ttcggccaac
      841 gagactcccc acgggccctg aacaacgtcg acaaatgggc ctaggtcccg acgcacgaga
      901 gcatggtcac gctggcggga cgtcgaagcg cgtgagtcat gcaatcaagt gctctaacgc
      961 cggggaacga tcaatcgcgc gccgggatca gggtttccag cgcgtctgcc atatagtcga
     1021 acaacatcgc gcgtcccatc gtgggctgca caacaatgcc atcgcacgaa cgccaaacca
     1081 tccagcgccg aaaagcgcgg tccttgtcgt aaagaccgag cagttcttca tattcgtcac
     1141 cttcgacgca gacatcgagg tcaatcgcca tgcgtaactc gtggaagtcg gcccatccct
     1201 gcagcatgag caggtcgggg atttcaaagg ccaagccggt cggtattttg acggtcgtac
     1261 ccggtagttg cactgaaacc tccttaatga cgaccgggat ccggccaccc actcacgaac
     1321 atgtgaatgt atgcctcacg ggaccgtgat cttgcaacag tttattttcg aagaaatagt
     1381 ctccgaacga ctcacggaac ggtgataaca aagcggatca ccgatcgccc gcaaggcatt
     1441 gaaaccaaga aacgccttgc agctatttaa attgatttca tattatttcg tcaatcgtat
     1501 taattccatc gttacgtttc caattgatca cgaatcctcg atttgtgaaa tcgatgcgat
     1561 cgcgttcagt tgattaaagt tggccgacga ctcagcgagt ggttatttcc ctcccaacag
     1621 gaaggaacac tcggtgcaaa aactgacttc aacggcccag tatgcggtcc gcccccaagc
     1681 ccgcgccgtc gcgacggcgc gtgatgagaa ttcgctgctg cgaggtttga cctatggcgt
     1741 cgtcctgtcg gcggctgtct ggatcgtcgc cggctatgtg acgtttgcac tgcgctaagg
     1801 tatttttagc caggtcatgc ccgcatgacc cacacgcttt aaacgttccc aggacttgct
     1861 ccttagacca cgaccgtttg aggctgaacc ctccacaacg ttcgatcatg gtctaagcct
     1921 atgtttagct tcgcgatcca cgccttccga ctgggtcagc ttcaggcgat cacactctaa
     1981 tctgctctgt ggtcgcaaac cgtaatcagg ggctgccgat cggtgcggcg cgatcggacg
     2041 ggggaacggg aacaattgtc cgcctccgcc gggtgaggct ttccaacgcc agataaacca
     2101 ccggcgtggt ataaagcgtc agcatttgtg acaccaacag cccgccgacg atggcgattc
     2161 ccagcggccg gcgtagctcc gagccggtgc caaatgctaa agccagcggc aatgcgccca
     2221 gcaaagccgc cagtgtggtc atcgtgatcg gacggaatcg ctccaggcag gcggcgtaga
     2281 tggcgtcgaa tggcgacatt ccgcgctggc gttcggcttc aagcgcgaaa tccaccatca
     2341 tgatggcgtt cttcttcacg atacccatca gcaggatgat gccaatcatg cccatgaccg
     2401 acaggtcggt tccggtaatc cacagcgcca gtaacgcacc cagccccgcc gacggcaacg
     2461 tcgagatgat cgtcagcggg tgcagtaggc tctcgtacag cacgcccagc acgatgtaga
     2521 tcgaaatcag cgcggcggcg atcagcagcg gctgggtgga cagcgactgc tgcatatctc
     2581 gcgcattacc ggcgaattcc gttcgcacgt cctcgggcat tcgcaattcg gcggttgaat
     2641 cccgtacggc tgtttcggcc gtgctcaacg ccacgccggg agcaaggttg aagctgatcg
     2701 tggcggctgg aaactgaccc tggtggcgca ccgcgagggg cgcagtagcg cgctcgaagc
     2761 gcgccacgga ggacagc
//
LOCUS       DASWQN010000765         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2448864, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000765 DASWQN010000000
VERSION     DASWQN010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2448864"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1383
                     /locus_tag="VGC82_12945"
     CDS             100..1383
                     /locus_tag="VGC82_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEY0424220.1"
                     /translation="MPNDTVIKLIQPGTFNDQLTDVLRDGARALLAQAVEAEVADFLG
                     TYADLKTEDGHRRVVRHGHLPEREVMTGIGPVAVRQPRVRDREAGADDPERIRFTPMI
                     LPPYARRSKSLEMLLPILYLKGISTGDFEEALAALLGKDASGLSASTIARLKGVWVDE
                     HKRWNERDLSAKRYVYIWADGIHLQARLEDDAQCILVVIGATPEGKKELIGFTDGTRE
                     SAHDWRTLLLDLKRRGLAIAPELAVADGALGFWKALGEVWPKTREQRCWVHKTANVLN
                     RLPKSQHAKAKRALQAIWMAEMKTDADAAFDAFIESYQVKYDKAAECLRKDREALLAF
                     YDFPAEHWKHLRTTNPIESTFATVRHRTIRSKGCLSNKTALAMVFKLVDGAQKTWRKL
                     DGHNQLPKIIQGVKFADGLEVVVKPAAIQAQTAAA"
BASE COUNT          311 a          492 c          487 g          254 t
ORIGIN      
        1 gagctattgc ccaaagttgg tgacggcatg aatcggtgaa ggcggcatat cgtgggggtg
       61 ttcaacgcct ccacttctcc atcgaaggag cgatatgccg tgcccaacga taccgtcatc
      121 aaactgattc agccagggac gttcaacgac cagctcaccg atgttttgcg cgatggggca
      181 cgcgccctgc tcgctcaggc agtcgaggcc gaggtcgccg attttctcgg cacgtatgcc
      241 gacctcaaga ccgaggatgg ccaccggcgt gtcgtgcggc atggccatct gcccgagcgc
      301 gaggtcatga ccggcatcgg gcctgtcgcg gttcgccagc cacgcgtgcg cgaccgtgag
      361 gccggtgccg acgatcccga gcgcatccgc ttcacgccga tgattttgcc gccctatgcc
      421 cggcgctcga aaagcctgga gatgctgctt ccgatcctct acctgaaggg catctcgacc
      481 ggcgacttcg aggaggcgct ggcggccttg ctcggcaagg atgcttccgg tttgtcggcc
      541 tcaacgattg cgcggctgaa gggtgtctgg gtcgacgagc acaagcgctg gaacgagcgc
      601 gatctctccg ccaagcgcta cgtctacatt tgggccgacg gcatccatct acaggcgcgt
      661 ttggaggatg acgcgcaatg catcctcgtc gtcatcggcg caacgccaga gggcaaaaag
      721 gagctgattg gcttcaccga tggcacgcgc gagagcgccc atgactggcg caccctgctg
      781 ctcgatctga agcggcgcgg gctggcaatc gcgccggagc tggccgtcgc cgacggtgcg
      841 ctcggcttct ggaaggcgct cggcgaggtg tggcccaaga cgcgggagca gcgctgctgg
      901 gtgcacaaaa cggcgaacgt cttgaacagg ctgcctaaga gccagcatgc gaaggccaag
      961 cgtgccttgc aggcgatctg gatggccgag atgaagacgg atgctgacgc tgctttcgac
     1021 gccttcatcg agagctacca ggtcaaatac gataaggccg ccgaatgcct gcgcaaggat
     1081 cgcgaggcgc tgctcgcatt ctacgacttc cccgccgagc actggaagca tctgcgcacg
     1141 acgaacccca tcgaaagcac cttcgcgacc gtgcggcaca ggacgatccg ctcgaaaggc
     1201 tgcctctcga acaagaccgc gcttgccatg gtcttcaagc tggtcgacgg cgcgcagaaa
     1261 acctggcgca agctagacgg ccacaaccag ttgccaaaga tcatccaggg tgtgaagttt
     1321 gccgacgggc tcgaagttgt cgtcaagccg gcagccattc aagcccaaac cgccgccgcc
     1381 tgacccatca ggccgtcacc aagaatcggc gatagctcaa ccagctgcgc tcagcctcaa
     1441 aggctggcgc gatcgcggac caaaggcggc aaggctcgac acgagcagaa cgagtccgga
     1501 ctactccagt cggcggacct gccgagcgac ccccggcgcg cacg
//
LOCUS       DASWQN010000766         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1789783, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000766 DASWQN010000000
VERSION     DASWQN010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789783"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..553)
                     /locus_tag="VGC82_12950"
     CDS             complement(11..553)
                     /locus_tag="VGC82_12950"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEY0424221.1"
                     /translation="MNEAAPFVLTLTLDPDAQANFDCLRKAHFPSHRLVVGAHVTLFH
                     ALPGDLPAASAVASEAAAQQAFPVDVTGVRFLGRGVAFSLESLRLHRLRARFYQLWEA
                     RLTPQDRQRWQPHVTIQNKADTIVAKALYQRLRADFSPYQVVATGLGLWIYRNGPWEA
                     VGSFPFADKTHGLAASAITT"
     gene            complement(570..1502)
                     /locus_tag="VGC82_12955"
     CDS             complement(570..1502)
                     /locus_tag="VGC82_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0424222.1"
                     /translation="MFGFSRSQSIAFVVMLVLIVVSPFVLYPVFLMRVLCAALFASAF
                     NLMIGYVGLLSFGHAAFFGMGSYVAAWTMKVWHVDASIAIIFGGLTGGALGLVLGYLA
                     IRRSGIYFAMITLALAQMVYFFCLEAPFTGGEDGIQQVPRGYMLGFINLSNDMTMYWV
                     VAAVFLCGFLLVHRIIHSPFGQVLKGIRENEPRATSLGYRTDDYKLIAFVLSCTIAGV
                     AGGTKALVFGIATLTDVHYSTSGEVVLTTLLGGLGTVFGPAMGALVVTSMENYLAQFG
                     AWVTVTQGVIFMICVLAFRRGIIGELGAFLKISL"
BASE COUNT          335 a          481 c          478 g          230 t
ORIGIN      
        1 cggcctcgga tcaggtagtt atcgcggaag cggcaaggcc gtgtgtcttg tcagcaaatg
       61 gaaaactacc gactgcttcc catgggccgt ttcggtagat ccacaggccc aggccggtcg
      121 cgacgacttg gtacggcgag aaatcggctc gcagtcgctg atacagcgct ttagccacga
      181 tggtatcggc cttgttctgg atggtcacat ggggttgcca ccgctggcga tcctgtggtg
      241 tcaggcgggc ttcccagagc tggtaaaacc gcgcgcgcag ccggtggagc cgcagcgatt
      301 ccagggaaaa ggcgaccccg cggccgagga accgcacacc tgtcacgtca acggggaaag
      361 cctgctgggc ggctgcctcc gacgcgacgg ccgacgcggc cggcagatcg ccgggcagcg
      421 cgtggaacag cgttacgtgc gcgccgacga ccaaccggtg ggatggaaaa tgtgccttgc
      481 gcaagcaatc gaaattcgcc tgcgcgtcgg gatcgagcgt cagcgtcagg acgaatggtg
      541 ccgcttcatt cacgcggttg ccgaaccggt tacagcgaaa tcttcaggaa cgcaccgagt
      601 tccccgatga tgccgcggcg gaacgccagc acgcagatca tgaagatcac gccctgggtc
      661 accgtcaccc aggcgccgaa ctgggccagg tagttctcca tcgacgtcac caccagcgcg
      721 cccatggccg ggccaaaaac cgttcccagg ccacccagca gcgtcgtcag cacgacctct
      781 ccggatgtcg agtaatgcac atcggtcagt gtggcgatgc cgaagaccag tgccttggtg
      841 cccccggcga caccggcgat cgtgcaggac agcacgaagg cgatcagctt gtagtcgtcg
      901 gtgcggtaac cgagcgaggt cgcccgcggc tcgttttcgc ggatgccttt cagcacctgt
      961 ccgaacggcg aatggatgat gcggtgcacc agcaggaagc cgcacagaaa cactgcggcg
     1021 acgacccagt acatggtcat gtcgttcgac agattgatga agcccagcat gtagccgcga
     1081 ggcacctgct ggatgccgtc ctcaccgccg gtgaacggcg cttccaggca gaagaagtaa
     1141 accatctgcg ccagcgccag ggtgatcatg gcgaagtaga tgccggagcg gcggattgcc
     1201 aggtagccca gcaccagacc tagcgccccg ccggtcaggc cgccgaagat gatcgcgatc
     1261 gacgcatcga cgtgccacac cttcatggtc caggcggcga catagctgcc catgccgaag
     1321 aacgccgcat ggccgaagga caacagcccg acatagccga tcatcaggtt gaaggcgctg
     1381 gcgaacaacg ccgcgcacag cacccgcatc aggaagaccg ggtacagcac gaacgggctg
     1441 acgacgatca acaccaacat caccacgaag gcgatggatt gcgaccgcga aaatccaaac
     1501 atcaggcggc ctttccaaac agtc
//
LOCUS       DASWQN010000767         6032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_732837, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000767 DASWQN010000000
VERSION     DASWQN010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732837"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(558..2783)
                     /locus_tag="VGC82_12960"
     CDS             complement(558..2783)
                     /locus_tag="VGC82_12960"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY0424223.1"
                     /translation="MNAIIGQPISRVDGPAKVTGSATYAGEFNLPGLAHAAMVLSTIP
                     HGRIVRMDTVEAERAPGVLAVLTHQNAPRLAYKQMPQRPQVDTKSGEQLHVLQDPDVK
                     FNGQPIALVVAETLEQAIHAADLVKVEYERSEAHTVFDTGRERPPSEVTVKGGRPADA
                     GRGNADAALEAAPVRMEATYVHPREHHNAIEPHVTIAHWEGEHLTLYDKTQWVDNDRK
                     EIAYIFDIPEQHIRVVSPFVGGAFGSALRTWPHVTLAALGARHVRRPVRLELTRRQLY
                     TSIGFRPHSQQHVALGATPEGRLTAVVHEVTQQVASYEEYAETVLSPTLHLYTCPDLR
                     AQYRLVEMDTNSPCPMRAPGVVTGVLGLEMAMDELAVKLDMDPLELRLRNYAERDENK
                     DLPWSSKELRACYRTAAERFGWERRRKEPGSMREGRMLVGYGMATAIYHSDRSAGSAR
                     AMLTGNGTAVVRSASSDMGPGTYTSMTQVAAETLGLPVDHVRFELGDTDLPYAPVHGG
                     STTMASVGNAVAAACQAMQRKLVDLARAAAEGPFAGLPADSITCRDGGLVRVDGRGAL
                     VPYAELLRQHNLSYLEAEATAEPGEETKKFSSAAFGAVFVEVRVDPELGSVRVPRIIG
                     AYDVGRVINPKTARSQCIGGMVNGVGMALQEEAQWDPRFGRVMNANLAEYLVPVCADV
                     QELEAIFVPGEDLNFNPLGVKGLAEVAICGVAPAIANAVFNATGRRLRNLPITPASLL
                     G"
     gene            complement(2780..3766)
                     /locus_tag="VGC82_12965"
     CDS             complement(2780..3766)
                     /locus_tag="VGC82_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY0424224.1"
                     /translation="MQPFTYVAAPDLSAAGARALHGAEFIAGGTDMLQLLQESVRRPK
                     ELADINALPISGISREPDGAVRIGALTRLQEAADSPIIREHFAVVAEALDATASPMVR
                     HMATVGGNLLQRTRCLYFRDVSTPCNKREPGSGCSAMEGENRIAAILGGSEHCIAVQP
                     SDLPVALIAMDAEVLVQHADGADHWVPIEDFHRVPGDTPDIETVLRPGDLIMALRLPA
                     HAAGRRSHYVKVRDRASFAWALASCAAALRQDADGTVVDVRLAAGGVATKPWRLPQAE
                     QVLLGHKLDREVIQRAAEAATTGADPRPLNAFKVPLLRQTVERVLLELGEPA"
     gene            complement(3770..4252)
                     /locus_tag="VGC82_12970"
     CDS             complement(3770..4252)
                     /locus_tag="VGC82_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0424225.1"
                     /translation="MQDSSLTFDIALTINGHSQGVNVDARTTLLDALRDRLHLTGTKK
                     GCNLGQCGACTVILDGRRVVSCLTLAVMCQGKSVTTIEGLADGGRLHPVQEAFVEHDA
                     FQCGYCTPGQIMSAVGAIQEGRVGSDDEVREQMSGNICRCGAYSNIVAAVRQAAGQGA
                     "
     gene            5114..5896
                     /locus_tag="VGC82_12975"
     CDS             5114..5896
                     /locus_tag="VGC82_12975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY0424226.1"
                     /translation="MTEREDPDKTGPQGGSTRRELLATTAVAVVGLIAAPPRDAAARS
                     YSGGLPWTPGEGQYPEVVQPVPWTFFTPAEGAAVEAIVDRLVPADNLSPGGKDAGCAV
                     FIDRQLAGPFGRAQGLYMRGPFAEGLPQQGLQSPTTPAERYRSALAALDRYCQQQQGG
                     KAFAALSPEQQDQILHGIEDGHVQLPETDGTAFFELVLQNTMEGFFADPIHGGNRDMV
                     AWKMLGFPGARYDYRAFIGNYNKPFPLPPVSIKGRPEWSRRT"
     gene            5900..>6032
                     /locus_tag="VGC82_12980"
     CDS             5900..>6032
                     /locus_tag="VGC82_12980"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HEY0424227.1"
                     /translation="MATKLPAKDVVVIGLGWAGSIVANELTDAGLNVVAIERGPGVTR
                     "
BASE COUNT         1037 a         2022 c         1912 g         1061 t
ORIGIN      
        1 cgacgcgaat tgcagctctg gcaagaggtg cgtcgccgcg ccgaggaagc gacgagtagg
       61 gcgcattcac gctgaccgga aaagatgcga gccaatttgc cgcttggcat cgttgtgtcg
      121 tggccgtgct tggtgcggcc acccacggct ttctttgcag ccatgagaaa actctgcttc
      181 cagtcaccgt gaatcgcccc aaccgcgtct gggtcgggaa tgataatacc gccgccggac
      241 tggctgcttc gttgctcagc gcctctccgc gtccgctgac gaagaggagg tttatgcact
      301 cccgatttac gccgaccgtg tgcccgtcag cttggtgatt ggcgagccgg aaccgtgccc
      361 tcggctgacg gagggcgtcg cacgtccgga cgcgcttgcc ccggctaccg caccgggcac
      421 ccgcttgagg aagcgctggc ggagacgcac gcggtgacct gtcccccgtg accccaaaat
      481 gttcccgccg ccgccctcgc cagcctgccc cgctcgccag ccctggtcca ttcgccagcc
      541 ctggcccact cgccagccta ccccagaaga ctcgctgggg tgatcggcag gttacgcaaa
      601 cgccgaccgg tcgcgttgaa cacggcgttg gcgatcgctg gtgccacgcc gcaaatagcg
      661 acttccgcca agcccttcac accgagggga ttgaagttga ggtcttcgcc cgggacgaag
      721 atcgcctcca gctcctgcac gtcggcgcag accgggacaa gatactccgc caggttggcg
      781 ttcatcaccc ggccgaacct cgggtcccat tgcgcctctt cctgcaacgc cattcccacc
      841 ccgttgacca tgccgccaat gcactggctg cgggcggtct tcgggttgat cacccgcccg
      901 acgtcgtagg cgccgataat ccgcgggacc cgcaccgaac ccagctccgg gtccacccgc
      961 acctcgacga acaccgcacc gaacgccgcg cttgagaact tcttggtttc ctcgccaggc
     1021 tctgccgtcg cctcggcctc caggtaactc aggttgtgct gccgcagcag ttcggcatag
     1081 ggcaccagcg caccgcggcc atcaacgcgc accaggccgc cgtcgcggca tgtgatgcta
     1141 tctgctggca gtccggcaaa tggtccttcg gctgctgccc gggcgagatc gacgagtttg
     1201 cgctgcatcg cctggcaggc ggcggccacc gcgttgccga cgctcgccat ggtggtcgat
     1261 ccgccatgca cgggagcgta gggcaggtcg gtgtctccga gttcgaagcg gacatggtcc
     1321 accggcaggc ccagcgtctc ggcagccacc tgggtcatcg acgtgtaggt cccaggcccc
     1381 atgtccgatg aggcgctgcg cacgacggcc gtgccgtttc ccgtcagcat ggcccgagcc
     1441 gaaccggcgc tgcggtcgga atggtagatg gcggttgcca tcccgtagcc caccagcatc
     1501 cggccttcac gcatcgaacc aggctcttta cggcgccgtt cccagccgaa gcgttcggcc
     1561 gcggtacggt agcaagcccg cagttccttg ctcgaccagg gcaaatcctt gttctcgtcg
     1621 cgttcagcgt agttccgcag acgcagctcc agcgggtcca tgtcgagttt gacggcaagc
     1681 tcgtccatcg ccatttcgag tcccaacacg cccgtcacga cgcccggcgc ccgcatcggg
     1741 cacggcgagt tggtgtccat ctcgaccagt cgatactggg cgcgcaggtc ggggcacgtg
     1801 tagaggtgca gggtcggtga cagcaccgtt tcggcatatt cctcgtagga ggcgacctgc
     1861 tgcgtcactt cgtggaccac cgccgtcagc cgtccctcgg gcgtcgcacc gagcgcgacg
     1921 tgctgctggg agtgcggccg gaatccgatg gaggtataaa gttgccgccg cgtcagctcc
     1981 agccgcaccg gccggcggac atgccgcgcc ccgagggcgg ccaacgtcac atgcggccac
     2041 gtccgcaagg ccgacccgaa cgcgccgccg acgaagggcg atacgacccg gatgtgctgc
     2101 tccgggatgt cgaaaatgta ggcgatctcc ttgcggtcgt tatctaccca ttgagtcttg
     2161 tcataaaggg tgaggtgctc gccttcccaa tgcgcgatgg tgacgtgcgg ctcgatcgca
     2221 ttgtggtgct cgcgcgggtg gacataggtc gcctccatcc ggaccggcgc cgcttccagc
     2281 gcggcatccg cgttcccgcg cccggcgtcg gccggccggc cacccttcac cgtcacctcg
     2341 ctgggggggc gttcgcgtcc ggtatcgaaa accgtatgcg cctcggaccg ctcgtattcg
     2401 accttgacca gatcggcggc gtgaatcgcc tgttccaagg tttcggcaac gacaagagcg
     2461 atcggttggc cgttgaactt cacgtccggg tcctgaagca catgcagttg ctcacccgac
     2521 ttcgtatcca cctggggacg ctgcggcatc tgcttgtagg cgagccgcgg cgcgttctgg
     2581 tgggtcagaa ccgccagcac gcccggggcg cgttccgcct ctaccgtgtc catacgtacg
     2641 atccggccat gcgggatcgt gctgagcacc atcgcggcat gcgcgagtcc aggcaggttg
     2701 aattcgccgg cataggtggc gctgccggtt accttggccg ggccatcgac ccggctgatc
     2761 ggctgcccga tgatcgcgtt catgccggtt ctcccagttc gagcagcact cgttccacgg
     2821 tttgccgcag aagcggaacc ttgaatgcat tcagtggacg aggatcggcc cccgtcgtcg
     2881 ccgcttcagc cgcgcgctgg atcacctccc ggtccaactt gtggcccagc agaacctgtt
     2941 ccgcctgtgg cagccgccag ggcttggttg ccacgccgcc ggccgcgagc cgcacatcca
     3001 ccaccgtacc atccgcgtcc tgccgcagag ccgcggcgca tgaggccagc gcccaggcga
     3061 aactggctcg gtcgcgcacc ttcacgtaat gggaccgccg tccggccgca tgcgccggaa
     3121 gccgcagcgc catgatcagg tcgccgggcc ggagcacggt ctcgatatcc ggcgtgtcgc
     3181 ccggcacccg atggaaatcc tcgatcggta cccaatgatc ggcgccgtcc gcatgctgaa
     3241 ccagcacctc ggcatccatg gcgatcaacg cgaccggtag atccgagggc tgcaccgcaa
     3301 tgcaatgttc gctgccgccg agaatggcgg caatgcggtt ttcgccctcc atcgcggagc
     3361 agccgctgcc gggctcgcgc ttgttgcaag gcgtcgagac gtcccggaaa tacaggcagc
     3421 gcgtccgctg caacaggttg ccgccgacgg tggccatgtg ccgcaccatg ggtgaggcgg
     3481 tggcatccag ggcctcggcc accacggcga agtgctcgcg gatgatgggt gagtcggccg
     3541 cctcctgaag ccgcgtcaag gcaccgatgc ggacggcgcc gtcaggctcc cgcgagatgc
     3601 ctgagatcgg cagggcgttg atatccgcga gttccttcgg cctgcgaacg gactcctgca
     3661 ggagctgcag catgtcggtg ccgccagcga tgaattcggc gccgtgcaag gcccgggcac
     3721 cggccgcgga caggtccggc gctgcgacat aagtgaaggg ctgcatacgt caggctccct
     3781 ggccggctgc ctggcgaacc gcggcgacaa tgttgctata ggcgccacag cggcagatgt
     3841 tcccgctcat ctgttcgcgg acttcgtcat ccgacccgac gcggccttcc tggatggcgc
     3901 ccaccgccga catgatctgg ccgggcgtgc aatagccgca ctggaaggcg tcgtgttcga
     3961 cgaaggcttc ctgcacggga tgcagccggc cgccgtcggc aaggccctcg atcgtcgtca
     4021 ccgacttgcc ctgacacatc acggcgaggg tcaggcagga cacgacgcgc cgcccatcga
     4081 gaataacggt acaggcaccg cactggccga ggttgcagcc cttcttggtg ccggtcagat
     4141 gcagtcggtc ccgcagcgcg tcgagcaagg tggtgcgcgc atcgacgttc acgccctgtg
     4201 agtggccgtt gatggtaagc gcgatgtcga aggtgagtga cgagtcttgc atggatggct
     4261 cctcggcggt ggcggcaacg tgggctggac gaagccgcaa cgctgaaacg ctgcaatgcg
     4321 ttcgccgcgt atgaccgcca acccggagaa atcgcagttg cttcgttcaa gccaatttga
     4381 cgcgacttag aagcagggga tgctcctcgg ccgacaatag gttccatacg ggttgatccg
     4441 cttcgcatga tatagaacca attgcgccaa tgccggcttc gtttgccggt tccaagaaaa
     4501 gccccagtca ttgttgccgg tgaccacttt gcggaatgca ttcgccgcca tcgggcattc
     4561 ccgaaccgag gctcatctcg agcggcatgt atctgaatgt attggcaggc gtctgttaca
     4621 aatgcgtgtg cctccggtgt cgggttgagc cgccaaaact gaccaatatg tgttcgcgat
     4681 ctcggatcat gtgaggacgt cgttcggcaa cgcggcatcc ggcaccacgt tggcgactgg
     4741 acgcacgtcg atagatggtc attgtcgtga agctgagttt cgtttttccc ctactatgta
     4801 ttttcgcggc cgcgccggtc tgcctggcgc aaacgagcac cggttcatcg gcaggtattt
     4861 caggggccaa cggcaacgcg ggtaccggcc aaaccccaac gccgagcggc aatcgctctc
     4921 ccgcggtagc ccgcggccca gacgagccat ccctcaagcc gcgccggcca gagactgcca
     4981 acggcacaag tggcgcgccg acaaatgaaa gctgcacccc cggaccgaac accagccgag
     5041 ggtcgagcgg cgcgaatggc tcgtgcttcc cacaatgaga tcgaatcagt aacgatctgc
     5101 aacggagcat tctgtgacag aacgcgaaga cccggacaag actggtccgc agggcggaag
     5161 cacacgccgg gaacttcttg ccacaaccgc cgtggcggtt gtcggtctga tcgcagcgcc
     5221 tcctcgtgat gccgctgccc gctcatattc cggcggattg ccctggaccc ccggcgaagg
     5281 tcagtatccg gaggtcgtac agcccgtgcc ttggacattc tttacccccg ctgagggggc
     5341 cgccgtcgag gccatagtcg atcggctcgt gccggccgat aatctcagcc cgggcggtaa
     5401 ggacgcgggg tgcgccgtgt tcatcgaccg ccagttggcc gggccattcg gccgagcaca
     5461 gggcctgtac atgcgcgggc cgttcgccga gggtttgccg cagcagggcc tgcagtcccc
     5521 gacaacgcca gccgaacggt atcgaagcgc actcgcggcg ttggatcgtt attgtcagca
     5581 gcaacagggc ggcaaggcct tcgccgcgct gtcgcccgag cagcaggacc aaatcctgca
     5641 cggtatcgag gacggacacg tgcagctgcc ggaaaccgac ggcaccgcat tcttcgaact
     5701 ggtgttgcaa aacaccatgg agggcttctt cgccgatccc atccacggcg gcaaccgcga
     5761 catggttgcc tggaagatgc tgggctttcc cggtgctcgg tacgactatc gcgccttcat
     5821 cggcaactac aacaagccat tcccgctgcc cccggtcagc atcaaaggcc ggcccgaatg
     5881 gtcccggagg acatagcgga tggcgaccaa gctcccggcg aaggacgtcg tggtaattgg
     5941 ccttggctgg gccggctcga tcgtcgccaa tgaattgacc gacgcgggac tgaatgtggt
     6001 cgcgatcgag cgcggccctg gcgtgacacg gc
//
LOCUS       DASWQN010000768         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2317061, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000768 DASWQN010000000
VERSION     DASWQN010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2317061"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VGC82_12985"
     CDS             <1..607
                     /locus_tag="VGC82_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HEY0424228.1"
                     /translation="DVGVRRLLGDLAEAEKGHEKTAAKLTDQLLSPDVRAEEDRTRRR
                     VFVLQYVQPGLAGLMDGSVSTLAPLFAAAFATHQNWQTFLVGLAASIGAGISMGFAEA
                     LSDDGSLTGRGSPWLRGVTCGLMTTVGGLGHTMPYLVPDSWPNAFWIATAIAGVVVFF
                     ELWAIAYIRARFMDTPFLQAVFQIVLGGAIVLAVGILIGAA"
     gene            690..1217
                     /locus_tag="VGC82_12990"
     CDS             690..1217
                     /locus_tag="VGC82_12990"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY0424229.1"
                     /translation="MTDRLQYGTPAKVLHWLVAALLLVQYLIGWLMPDVHRGPPGVAM
                     TFHISFGILILTLIVLRFAWRLTHPVAPESSLPPWQRLSSEAMHWLLYLLVLATTLTG
                     WLFASFRGWSVSFFYLFPLPMLASDNRAAIKTIDGWHQAAEWALLITIGLHVVVALVH
                     IFVYRDRIMQRMLPG"
     gene            complement(1233..2099)
                     /locus_tag="VGC82_12995"
     CDS             complement(1233..2099)
                     /locus_tag="VGC82_12995"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0424230.1"
                     /translation="MKPGDHVVTCLSVFCGTCDNCSTGRPVLCTDTTVKMLPGASNRL
                     SWARPEKLNQFLNLSSFAEQMLVHENAIVKIRKDMPLELAALIGCGVITGYGAVVNTA
                     KVAPGESVVVIGCGGVGMAAINGAEIAGAGRIIAVDTNPAKLQLATKLGATDIVDPRN
                     GDVVQQVRDLTGGGVHHSFEVLGRKETAEQAFGMLAPGGTATIVGMIPFGQKIELHGF
                     DFLRERKIQGSSMGSNHFRVDMPRLVEFYMRGRLHLEDWISARLKLSEINDGFAAMKA
                     GKTVRSVIMFDS"
BASE COUNT          330 a          698 c          660 g          419 t
ORIGIN      
        1 ggatgtcggc gtccgcaggc tgctcggcga cctcgccgag gcggaaaaag gccacgagaa
       61 aaccgccgcc aagctgaccg accagctcct cagcccggac gtgcgcgccg aagaagaccg
      121 gacgcgacgg cgggtgttcg tgctgcaata tgtgcagccc ggtcttgccg gcctgatgga
      181 cggctcggtc tcgacgctgg cgccgctgtt cgcggcagcc tttgccaccc atcagaactg
      241 gcagactttt ctggtgggac tggccgcctc gatcggcgcc ggcatcagca tgggttttgc
      301 ggaagccctc tccgacgacg gatcgctcac cgggcgcggt tcgccgtggc tgcgtggcgt
      361 gacctgcggc ctgatgacga cggtcggcgg tctcggccac accatgccct atctcgttcc
      421 ggactcctgg cccaatgcgt tctggatcgc gaccgcgatt gcgggcgtcg tggtgttctt
      481 cgaactgtgg gcgatcgcct atattcgcgc gcgcttcatg gacacgccgt tcctgcaggc
      541 ggtattccag atcgtgctcg gcggcgccat cgtgctcgcc gtcggcattc ttatcggggc
      601 ggcatagcct gcgcggagcc gctgggcatc ccagtgccac gccggctttg ccattcgaac
      661 agcccctcgc aacctcctcc aggaatccga tgaccgatcg tctgcaatac gggacccccg
      721 ccaaggtgct tcactggctg gtggccgcac ttctgctggt gcaatacctg atcggctggc
      781 tgatgccgga cgtccaccgc ggcccgcccg gcgtggcgat gacgttccac atttcgtttg
      841 gaatcctgat cctgaccctc atcgtcctga gattcgcctg gcggctgacg catccggtgg
      901 cgcccgaaag ctcgcttcca ccctggcagc ggctgagttc ggaagcgatg cactggctgc
      961 tctatctgct ggtgctggcc actacgctga cggggtggct gttcgcgtcg tttcgcggct
     1021 ggtcggtgtc gttcttctat ctttttccgc tgccgatgct ggcttcggac aatcgggcgg
     1081 cgatcaagac catcgatggc tggcatcagg cggcggaatg ggcgctcctg atcacgatcg
     1141 gattgcacgt cgtcgtcgcg ctggtccaca tcttcgtcta tcgcgaccgc atcatgcagc
     1201 ggatgctgcc gggataggct tttggttcgg ttttagctgt cgaacatgat cacgctgcgc
     1261 accgtcttgc ccgccttcat cgcggcaaag ccatcgttga tctcggacag cttcagcctg
     1321 gccgaaatcc agtcctccag atgcagacgc ccgcgcatgt agaactcgac cagccgcggc
     1381 atgtcgaccc ggaaatggtt cgagcccatc gacgagccct gaatcttgcg ctcgcggagg
     1441 aaatcgaaac cgtgcagctc gatcttctgg ccgaacggga tcatcccgac gatggtcgcg
     1501 gtgcctcccg gcgccagcat cccgaacgcc tgctccgcag tctccttgcg gcccagcact
     1561 tcgaacgaat gatgcacgcc gccgcccgtg agatcgcgga cctgctgcac cacgtcgccg
     1621 ttgcgcggat cgacgatgtc ggtggcgccc agcttggtgg ccaattgaag ttttgcggga
     1681 ttggtgtcga ccgcgatgat gcggccggcg ccggcgatct cggcgccgtt gatcgcggcc
     1741 atgccgacgc cgccgcagcc gatcaccacc acgctctcgc cgggcgcgac cttcgcggtg
     1801 ttgacgaccg cgccgtagcc ggtgatcacg ccgcagccga tcagggccgc cagttcgagc
     1861 ggcatgtcct tgcggatttt gacgatggcg ttctcgtgca ccagcatctg ctcggcgaac
     1921 gacgacaaat tgaggaattg attaagtttc tccgggcgcg cccatgacag ccggttggac
     1981 gcgcccggca gcatcttcac cgtcgtgtcg gtgcacagca ccggccgccc ggtcgagcaa
     2041 ttgtcgcagg tgccgcagaa taccgaaaga caggtcacca cgtgatcgcc gggcttcaca
     2101 tagctga
//
LOCUS       DASWQN010000769         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1657901, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000769 DASWQN010000000
VERSION     DASWQN010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657901"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VGC82_13000"
     CDS             <1..200
                     /locus_tag="VGC82_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424231.1"
                     /translation="FHALGNEVSLPPDDRGRALDLDACSWRRWADFMIGGPLPAEPPL
                     PEMLRRIGQAAFYLSTIAERI"
     gene            complement(205..1728)
                     /locus_tag="VGC82_13005"
     CDS             complement(205..1728)
                     /locus_tag="VGC82_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEY0424232.1"
                     /translation="MAPPPVIRLHPEDNVVIARATLLPGASVGGNFLAKERIPAGHKV
                     AVRPIAGGEAIRRYGQIIGFASAAIAPGQHVHVQNCEMGDFAKDYAYGVDARATEFFE
                     PAATFQGIRRPDGKVATRNYIGILTSVNCSAHVAGVVADMFRRNPFSGDDPLADFPNV
                     DGVVALTHKTGCGMTQDEPLRVLRRTLAGYARHPNFSHVIVLGLGCEVNQIGGLMEEQ
                     RLAGRLKAMDIQTMGGSRKTVAAGVSFVKEILAESNQVTRVTVPARELTVALQCGGSD
                     GYSGVSANPALGAASDLLVRHGGTVILSETPETYGAEHLLTRRAVSREVGEKLVGLMR
                     WWEDYTKQEGAEMNANPSPGNKAGGLTTILDKSLGAMAKAGTTNLVDVVRYAEEVKAR
                     GFVFMDTPGYDPVAATGQVAGGANLVCFTTGRGSVFGCKPAPSIKLATNTPMFRHIED
                     DMDVNCGTVLDGDETVQQAGQRIFDMILRVASGERTKSELFDFGAAEFAPWVLGATM"
BASE COUNT          321 a          608 c          550 g          348 t
ORIGIN      
        1 tgtttcacgc gctgggcaac gaggtcagcc tgccgcccga tgaccggggc cgtgccctgg
       61 acctggatgc ctgctcctgg aggcggtggg cggatttcat gatcggcggt ccgctaccgg
      121 ccgaaccacc attgcccgag atgcttcgcc ggatcgggca agccgcattc tacctttcga
      181 caatcgccga acgcatctga cgcgttacat cgtggcgccc agtacccatg gcgcgaactc
      241 cgctgcgccg aaatcgaaca gttcgctctt ggtccgttcc cccgatgcaa cgcgcaggat
      301 catgtcgaaa attcgctgcc ccgcctgctg gaccgtttcg tcgccatcaa ggacggtgcc
      361 gcagttcacg tccatgtcgt cctcgatatg tctgaacatc ggcgtgtttg ttgccagctt
      421 gatcgacggc gcgggcttgc agccgaacac gctgccccgc ccggtggtaa agcacaccag
      481 gttggcacct cctgccacct gacctgtcgc ggcgaccggg tcgtatccgg gcgtatccat
      541 gaagacaaag ccgcgagcct tcacttcctc cgcgtatcga acgacatcga ccagattggt
      601 ggtgccggcc ttggccatcg cgcccaacga cttatccagg atcgtggtga ggccgcccgc
      661 cttgttgccg ggggatgggt tggcgttcat ctccgctccc tcctgctttg tgtagtcctc
      721 ccaccagcgc atcagtccaa ccagcttttc cccgacttcc cgagaaaccg ctcggcgagt
      781 cagcagatgt tcggcaccat aggtctcggg tgtttccgac aggatcaccg tgccgccgtg
      841 tcgtaccagc aggtcgctcg ccgcacccag cgccggattg gccgatacgc ccgagtaacc
      901 atccgatccg ccgcattgca gcgccaccgt cagttcacgg gccggcaccg tcacacgcgt
      961 gacctggttg gattccgcca agatttcctt cacgaacgac acgcccgcgg caacagtctt
     1021 gcggctgccg cccatcgtct gaatgtccat ggccttcaat cggccggcca ggcgctgttc
     1081 ttccatcaat ccgccgatct gattcacctc gcaacccaga ccaagcacga tgacgtggga
     1141 aaaatttggg tggcgggcat agccggccaa agtgcgccgc agcacgcgca gtggctcgtc
     1201 ctgcgtcatg ccgcaaccgg tcttgtgggt cagcgcaacg acgccgtcga cattgggaaa
     1261 atcggccagc ggatcgtcgc cgctgaacgg atttcgacgg aacatgtcgg cgaccacgcc
     1321 ggcgacatga gccgagcagt tcacgctggt gagaatgccg atatagttgc gcgtagcgac
     1381 cttcccgtcc ggccgtcgga tgccctggaa cgtcgccgcc ggctcgaaga actcggtggc
     1441 tcgggcatcg acgccatagg cataatcctt ggcgaaatcg cccatctcgc agttctggac
     1501 atgcacatgc tggcccggcg cgatggcggc cgaggcgaag ccgatgatct ggccgtagcg
     1561 tcgaatggcc tcgccgccgg cgatgggtcg gacagcaact ttgtggccag ccgggatacg
     1621 ctccttcgcc aggaaattgc cgccgaccga ggcgcccggc agcaacgtgg cgcgtgcgat
     1681 gacgacgttg tcctcggggt gcaggcggat gacgggtggc ggggccatga tgggttcctc
     1741 ggtgaagatc gccgtctttt acagcctccg cgtcatcatc cgcaaagccg gctgctaaac
     1801 gttcgcaaag ccggctgcta aacgttg
//
LOCUS       DASWQN010000770         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2185904, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000770 DASWQN010000000
VERSION     DASWQN010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2185904"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VGC82_13010"
     CDS             <1..738
                     /locus_tag="VGC82_13010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HEY0424233.1"
                     /translation="FLYKEVLKKELPWVENVQRAKRPAKLPVVFTPTEARAVLAKMRG
                     TTRLMAQLLYGCGLRLNECARLRVKDVDFGYLHITIRDAKGGRDRITMLPTSLVDPLR
                     HAIEKRRLLHEEDLATGHGAVFLPGAIARKYPNAAREFGWQYIFASSRVSCDPRMAEQ
                     SPGEGRHHVGESFLQRAVKQAVRDAALDKPGTCHTFRHSFATHLLENGYDIRTVQELL
                     GHKDVSTTMIYTHVLNKPGLGVKSPLD"
     gene            complement(894..>3219)
                     /locus_tag="VGC82_13015"
     CDS             complement(894..>3219)
                     /locus_tag="VGC82_13015"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY0424234.1"
                     /translation="GNHEAADFNGTWYAFLAHTYDGGANWTTVNATPNAPVQKEACIW
                     NEGGSNQCRNLLDFNEITMDEKGRVLYGFADGCIDDCEEGGPNSYSSKATIARQSGGK
                     GLLSQFDPTEPVAPQAACLSGRRDDLASYLKWIAPDNGGSDITGYKIYRGTTANNLVL
                     IGQQVGDKNTYNDRTANPAVPNYTYEIRAVNASGEGVFSNLLTLPLSPRVEKTGSCSA
                     PGVTAVTDPVGDESDGVTEHDLTSVSMAEPVTNAQTGAADNIVFTIKVQDLNPVPPGW
                     RWSVRFTVAGYNPPAAPAVGPQEDWFVSMISSDNAAPAFTYGTTGVFQGAARFFTTVG
                     NLDPSSTFNADGTITLVLPKETFRSHAVCTGTCGPLNPGTAVSIALGSVRLSPPSEVP
                     GSGGTNETIPDTTGAASYQLRPANLCLPNTAPVASLTADKGTGTAPLTVTFNGSASSD
                     DDSIDTIATYTFNFNDGTDDVVQSSPIVVHTFNQGGLYDVKLVVTDSRGKVSSNTAHA
                     LVEVEQTTTPSAAQLLNISSRLRAQTGDNTLIGGFIITGTGAKKVAVRALGPSTQVPG
                     FVPDTVLELHDSTGALVTSNDNWGDSPEASQIQAVGLAPGDSRESAILRLLNPGAYTA
                     IVRGKNNAIGTAVVEAYDLDPTNDSKLANLATRGFVQTGDNILIAGFITGNRNANINV
                     LLRAMGPSLAGQVPAALADPIMELHDSNGATLATNDNWQDTQKTQIEQTGIPPSNARE
                     SAIVSSLPPGGYTAIVRGVNGGVGNAVVEVYNLP"
BASE COUNT          588 a          917 c         1068 g          646 t
ORIGIN      
        1 ttcctctaca aagaagtgct aaagaaggag ctgccctggg tggaaaacgt acaaagggcg
       61 aagcgacccg ccaagctacc ggtggttttt acacccaccg aagcgagagc cgtcctggcc
      121 aagatgagag gaacgacccg gttgatggcc cagttgctct atggatgcgg gctgcgcttg
      181 aacgaatgcg cccggcttcg ggtgaaggat gtggatttcg gatatctgca cataaccatt
      241 cgggacgcga aaggtgggcg cgaccgcatc acgatgctgc cgacttcgct ggtggacccg
      301 ctccggcacg cgattgaaaa gcgccgactt ttacatgaag aagatctggc gacaggccac
      361 ggcgcggtct ttctgcctgg ggcaattgcg cgaaagtacc cgaacgcggc ccgggagttc
      421 gggtggcaat acatcttcgc ctcgtcgcgg gtctcttgtg atccgcgcat ggccgagcaa
      481 tctccggggg aagggcggca tcatgtgggg gagagctttc tccagcgcgc tgtcaaacag
      541 gccgttcgcg atgcagccct ggataagccg gggacctgcc acacctttcg gcactctttc
      601 gcgacgcatt tactcgagaa tggctacgat atccggacgg tccaggaact cctcgggcac
      661 aaggatgtaa gcacgacaat gatttacacc cacgtgctca acaagccggg tctcggggtg
      721 aagagtccgt tggactgagg agaaggaagg acgctgaagg tttgagacaa gcggtctcct
      781 tcgccaaggc tacggcgtgc cagggagcgc ttgcctaaca attattcctc gacggcgagc
      841 ggcatgcgcc cgcggcattt ctgccgcgga cgcactacgc acgcaaatcg gcgctaaggc
      901 agattataga cttccacgac ggcgttaccg acgccgccat tcacgccgcg gacgatggcg
      961 gtgtagccgc cgggcggcag cgagctgaca atcgcggatt ccctggcgtt gctaggtggg
     1021 ataccggtct gttcgatctg ggtcttctgg gtgtcctgcc agttatcgtt cgttgccagg
     1081 gtcgcgccgt tgctgtcgtg cagttccatg atgggatccg ccagggcggc agggacctgg
     1141 ccggcgagcg atggtcccat cgcgcgcagg agcacgttaa tgttcgcgtt ccggtttccg
     1201 gtgatgaagc cggcgataag gatgttgtcc cctgtctgga caaagccgcg cgtggcgagg
     1261 ttggccagct tcgaatcgtt ggttggatcc agatcgtagg cctcgactac cgcggtgccg
     1321 atggcgttgt tcttgccgcg cacaatggcg gtgtatgcgc ctgggttaag caaccgcagg
     1381 atcgctgact cgcgggaatc gcccggcgcc aatccgactg cctggatctg cgaagcctct
     1441 ggcgaatcgc cccagttgtc gttgctggtc acaagcgcgc cggtggagtc gtgcaattcc
     1501 aggacggtat cgggcacgaa ccccgggacc tgcgtggaag gcccgagggc gcggaccgct
     1561 actttcttcg caccggttcc agtgatgatg aatccgccga tgagggtgtt gtcgccggtc
     1621 tgggcgcgga ggcggctgga gatgttcagc aactgggcgg cgctcggggt ggtggtttgt
     1681 tccacttcca ccagggcatg ggctgtgttc gagctgacct tcccgcgcga atcggtcacg
     1741 accagcttca cgtcgtagag gccaccctgg ttaaaggtgt gcacgacgat cggcgaactt
     1801 tgcacaacgt catcggtgcc gtcattgaag ttgaacgtgt aggtcgcgat ggtgtcgatc
     1861 gaatcgtcat ccgacgacgc gctgccgttg aaagtcacgg tcaacggagc ggtgccggtt
     1921 cctttatcgg ccgtgagact ggccacgggc gcggtgttag gcaggcaaag gtttgccggc
     1981 cgcaactgat aactcgcagc gccggtcgta tccggaatgg tctcgttggt gccaccgctg
     2041 ccgggaactt ccgaaggcgg cgacaggcgc acgctcccga gagcgatgct aacagccgtc
     2101 ccgggattga gcgggccgca ggtgccggtg cacacggcat gcgaacggaa ggtttccttg
     2161 ggaagcacga gggtgatggt gccatcggcg ttgaaggtgc tcgacggatc caggttgccg
     2221 accgtggtga agaagcgcgc cgcgccctgg aatacgccgg tcgttccgta ggtgaatgcc
     2281 ggggcggcgt tatccgaaga gatcatggag acgaaccaat cttcctgggg ccccacggca
     2341 ggcgcggccg gcgggttgta tcccgcaacg gtaaagcgga cagaccagcg ccagcccggc
     2401 ggcacgggat tgagatcctg cactttgatg gtgaagacga tgttgtcggc ggcgccggtc
     2461 tgcgcgttgg ttaccggctc cgccatggag acggaggtga ggtcgtgttc ggtgacgcca
     2521 tcgctttcat cgcctaccgg atcggtgacc gcggtcacgc ccggcgcgct gcacgacccg
     2581 gttttttcca cccgtgggct gaggggcaag gtgagtaaat tgctgaatac accttcgccg
     2641 gacgcattca cggccctgat ctcgtaggtg tagttcggaa ccgcaggatt ggcggtgcga
     2701 tcgttgtagg tgttcttatc acctacctgt tgaccgatca gaaccaggtt attcgcggtg
     2761 gtgccgcggt aaatcttgta gcccgtgatg tcgctgccgc cgttgtccgg cgcgatccac
     2821 ttcaagtacg aggcgagatc gtcgcgccgg cctgagagac aagccgcctg cggcgccacc
     2881 ggctcggtgg gatcaaactg cgagagcaat ccctttccac ccgattgacg cgcgatcgtc
     2941 gccttcgagc tataggaatt cgggccgcct tcctcgcaat cgtcgatgca accatcggcg
     3001 aagccgtaaa ggacccggcc tttctcgtcc atggtgattt cattgaagtc gagcagattg
     3061 cggcactggt tgctgccgcc ctcgttccag atacaggctt ctttctggac cggcgcgttc
     3121 ggggtggcgt tgaccgttgt ccaatttgcg ccgccgtcat aagtatgggc gaggaaggca
     3181 taccaggtgc cgttgaaatc cgcggcctca tggttgccg
//
LOCUS       DASWQN010000771         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_338867, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000771 DASWQN010000000
VERSION     DASWQN010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338867"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VGC82_13020"
     CDS             <1..623
                     /locus_tag="VGC82_13020"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424235.1"
                     /translation="YQYAGVRSLGDDEIHVRTYHFILPFHQIRPSRTPSGLWVDAGHI
                     WVPMDDGTTMVYNWNYSAGTVGLTEEDRLERHLGNGPLHVDQTTFRSKANRDNNYFLD
                     REVQRTESFSGIDGINAQDRALQESMGRIVDRSKEHLGPSDKAIIQARKLLREAVQAV
                     AAGKPPHGTGSSYYGLRAHEAVLPRDADWRQELAADLKQEAILATV"
     gene            767..1000
                     /gene="xseB"
                     /locus_tag="VGC82_13025"
     CDS             767..1000
                     /gene="xseB"
                     /locus_tag="VGC82_13025"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY0424236.1"
                     /translation="MAEDDVAQLSFEDALAELDQIVRGLEGGTLKLDAAVQAFERGVM
                     LRRHCEAKLAEVEARVEALVQRTDGSFGTRPLE"
     gene            997..1767
                     /locus_tag="VGC82_13030"
     CDS             997..1767
                     /locus_tag="VGC82_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY0424237.1"
                     /translation="MVKSRADQLLVARGLVESRTRAQALILAGKIFSGERRVAKAGDF
                     LADDVPLEVRGQDHPWVSRGGLKLAHGLAHFGFSPAGRVCLDVGASTGGFTDVLLANG
                     AAKVHAVDVGHGQLAWKLRCDPRVVVHEKTNARYLTREAVADPIEALVCDASFIGLAT
                     ILPAPLALCAAGAWAVVLIKPQFEAGAASVGGKGVVRDPAVHQAVCERVIDWWSGLPG
                     WTVSGVIESPITGPEGNKEFLLGAHLLATNASSEAFRG"
     gene            2126..>3014
                     /locus_tag="VGC82_13035"
     CDS             2126..>3014
                     /locus_tag="VGC82_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0424238.1"
                     /translation="MLGTVALAVDPGTEAPNDWPQYNRTANAWRYSPLDQINKDNVGK
                     LSVAWIAHGGDITMGIQETPLAIDGVIYSITSGDKVAALDGKTGQEIWRYEPKLHALT
                     KKVLFSPYSRGVAVGHGMVFIGTVDGRGIALDQKTGKEKWQVQLTDFVNCHGCNFTSP
                     PVVAGDMLTFGSTAGELATQGKIFGVEAATGKKVWEFNTIKQDEKSWPGESGKYGGGG
                     AWMPGTYDADTDTVYYGTGNPGKDFVTSDREGDNLYTDSIIALNPRTGSIKWYRQEIK
                     QDVWDYDSPYEVMLFKKNGK"
BASE COUNT          628 a          841 c          999 g          546 t
ORIGIN      
        1 gctaccagta cgctggcgtc cggtcgttgg gagacgacga aatccacgtc cgtacctatc
       61 atttcatcct gccgttccat cagatacggc cgtcaaggac gccaagtggg ctttgggtgg
      121 acgccggcca catctgggta ccgatggatg acggcaccac catggtttac aactggaact
      181 acagcgccgg gacggtgggc ctgaccgagg aagaccggct ggaacgtcat ttgggcaacg
      241 gtccgctgca tgtcgatcag acgacgttcc gatccaaggc gaaccgggac aacaactact
      301 tcctggaccg tgaggttcaa cggaccgaaa gcttctccgg aatcgacggc atcaacgcgc
      361 aggaccgtgc cttgcaggaa agcatggggc ggatcgttga ccgctccaag gagcatctgg
      421 ggccgtcgga caaggcgatc atccaggccc gcaagctgct gcgggaagcg gtgcaggcgg
      481 ttgccgccgg aaagccgccc cacggaacgg ggtcaagcta ttatggcctg cgggcgcacg
      541 aggccgtact cccgcgcgat gccgactggc ggcaggaact ggccgcggac ctgaagcagg
      601 aagcaatcct ggcgacggtt taagcgaccg gttgcccccg tcgggctgac gccttaagtg
      661 agcctggtca aggaggcgtc gccttcgcgt gcccgtaatc tgccccggcg aatgtacggg
      721 cggtcccgtg gccagtcttc aaccagtcgg aaaggcccgc gaaatcatgg cggaagacga
      781 tgtcgcccag ttgtcgtttg aagacgcgtt ggcggagctg gatcagatcg tccgcggact
      841 cgaaggcggc acgctgaaat tggacgccgc ggtacaggcg ttcgaaaggg gcgtgatgct
      901 gcgccggcat tgcgaggcca agctggcgga agtcgaagcc agggtggaag cgctggtgca
      961 gcggaccgac ggttcgttcg gaacacggcc gctcgaatag tgaagtccag ggccgatcaa
     1021 ttgctggtgg cacgtggctt ggtcgagagc cgcacgcggg cgcaggcgct gatcctggcg
     1081 gggaagatct tctccggcga gaggcgggtt gccaaggcgg gcgacttcct ggccgacgac
     1141 gtgccgttgg aggtgcgcgg gcaggatcat ccttgggtgt cgcgtggtgg cctgaaactg
     1201 gcgcatggat tggcgcattt cgggttttca ccggcagggc gggtgtgcct ggatgtcggc
     1261 gcctcgacgg gcggatttac cgacgtgctg ctggcaaatg gcgccgcaaa agttcatgcg
     1321 gtcgatgtcg gccatgggca acttgcctgg aagctgcgct gcgaccctcg ggtcgtggtg
     1381 catgagaaaa ccaacgcccg ctatctgacg cgggaggctg tagccgatcc gatcgaagcc
     1441 ctggtttgcg atgccagctt catcgggttg gcaacgatat tgcccgcgcc cttggcattg
     1501 tgcgccgccg gtgcgtgggc ggtcgtcctg atcaagccgc agttcgaggc cggggccgca
     1561 tcggtcggcg gcaaaggtgt ggtgcgcgat cccgcggtgc accaagcggt ttgtgagcgt
     1621 gtaattgact ggtggtccgg gttgcctggc tggaccgtct cgggggtcat cgaaagccca
     1681 atcaccggac cggaaggcaa caaggagttc ctgttgggcg cgcatcttct tgcgaccaac
     1741 gcttcttcgg aggcatttag gggctgactt cgaacgcgaa cggcgacagg actaccgata
     1801 gctgccgcag ttaaagcgtg ccggcgccgg ctatgcatag gcatctggcc acgacccggt
     1861 tgatctgcgt cgtacagggg cgcatcctcc gacgtagaat gcgacatccc agtggtatcg
     1921 tcgttcgatc gccgattcga gcaaatcaat gtggcggtat cttgcgagcc agtacaagtg
     1981 gcgtcacgaa tgactgtagt ttcaactcgc cgtcttatga acaaaaatcg ctagatgcag
     2041 gagcagtcgc taccgtccgc actaaacagg gaggagacaa acaatgcaca agatagcttt
     2101 ggcggcaacc gttgggttgg cttgcatgct tggcacggtc gccctcgcag tggaccccgg
     2161 gaccgaggcc cccaacgact ggccgcaata caaccgaacc gccaacgcgt ggcgttacag
     2221 tccactcgac cagatcaaca aagacaatgt cggcaaactc tcggtagcct ggatcgccca
     2281 tggcggcgac atcaccatgg gcattcagga aacgccgttg gcgatagacg gtgtgatcta
     2341 ctccatcacg tcgggcgaca aggtcgccgc gctggacggc aaaacggggc aggagatttg
     2401 gcgatatgag cctaaactcc atgcgctcac gaaaaaggtg ctgttctcgc cctatagccg
     2461 gggcgtcgcg gtcggccacg gcatggtctt catcggtacg gtggatggcc gcggtatcgc
     2521 ccttgaccag aagaccggca aggagaaatg gcaggtccag ttgaccgatt tcgtcaactg
     2581 tcacggctgc aacttcacgt cgccgccggt ggtggccggc gacatgctga ctttcggatc
     2641 aaccgcgggt gagctggcga cgcaaggaaa gatctttggt gtcgaagccg ctaccggcaa
     2701 gaaggtttgg gaattcaaca ccatcaagca agacgagaag agctggccgg gcgaaagcgg
     2761 gaaatatggc ggcggcggcg cctggatgcc gggaacatat gatgccgaca ccgacaccgt
     2821 ttactatggc accggcaatc ctggaaaaga ctttgtcact tcggatcggg agggcgacaa
     2881 cctttacacc gactcgatca tcgcgctcaa tccccggacc ggaagcatca agtggtatag
     2941 acaagaaatc aagcaagatg tgtgggatta tgattcacct tatgaggtga tgctgttcaa
     3001 gaaaaacggc aagg
//
LOCUS       DASWQN010000772         3301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2054086, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000772 DASWQN010000000
VERSION     DASWQN010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054086"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /gene="arfB"
                     /locus_tag="VGC82_13040"
     CDS             complement(<1..284)
                     /gene="arfB"
                     /locus_tag="VGC82_13040"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY0424239.1"
                     /translation="MLSIDERAVQETFIRASGPGGQNVNKVETAVQLRLSLDRAALPP
                     SVRARLEKLAGRRLTLDGDILITSQQHRTQERNRAEAMNALVELIRRATVA"
     gene            498..1835
                     /locus_tag="VGC82_13045"
     CDS             498..1835
                     /locus_tag="VGC82_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424240.1"
                     /translation="MKTAITRRKAVGLGIAAGTLPLVNIHSAGAAGRLSVALWDHWVP
                     AGNQAMKQMVGAWAEKNKVDVTLDFLTSVGNKITITMAAEAQAKTGHDIYAFDMWSVH
                     QYADQLDPMDDIMQAFIAKYGKIGRAYEYLGVSEGHWRAVPVAWGSSPLTPCGRISML
                     KQYADIDLQKWFPAHEATPATSAEWTYDAQLKAAAACHKAGFPFGFGCGTTTDSNQTW
                     GAMFGAFGADLVDAKGAITADSDNVRAALEYAKRMVPFLPSDTVSYDDASNNRALISG
                     RSALIWNPPSAWAVAKRDAPQVAADCWTFPNPRGPAGRMVPIRPYFFGLWSFAQNKPA
                     ARDLLAHLGAREQMETLTTAVDGYDIPPYASMADFKIWEEVGPPKGTVYNYPIRPWHD
                     AEYYVTGSSGPPDIGVQAWNRGLIPTMVSKIVAGQTIQQSIDWAKDELEGFTR"
     gene            1968..3023
                     /locus_tag="VGC82_13050"
     CDS             1968..3023
                     /locus_tag="VGC82_13050"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEY0424241.1"
                     /translation="MTLTALLRHLGFAALLVLLSAATVRMMIAARVMDTPEARKAHDR
                     PTPKGGGVGVVVTFLVGLAVLYRYAEFARLANPYFLGVIEASVAIAVVAFLDDLFDWP
                     FIVKLGAQVLAALVAVGSGLYVSDFRIPYLGPLAFAWIGVAATVFWVLFTTNALNFID
                     GLNGLASGVALVACLFIVFIAEQHGGWFAYAAAGLLAAGLAGFLPFNFPKARIFMGDV
                     GSQFCGFMLAVLAVVASRFDGVELSFLLMPMLLSGVLFDVAFTLVRRTLAGETLTQPH
                     RGHLYQVAQRAGVSPVAVTLVHWGFAVFGGVCCLLFIALPSPYKPPVPFITVVPQLCW
                     VWFVADRAHRAGLSRWG"
     gene            complement(3105..>3301)
                     /locus_tag="VGC82_13055"
     CDS             complement(3105..>3301)
                     /locus_tag="VGC82_13055"
                     /inference="COORDINATES: protein motif:HMM:NF021900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424242.1"
                     /translation="VTALVPALGYEASSRVAKRALAERRSVADVVLDEGLLTEVQLNE
                     LLQLEAMTRPSRTRVVVARA"
BASE COUNT          554 a         1083 c         1053 g          611 t
ORIGIN      
        1 gcaaccgtcg ctcgccggat cagctcgacc agcgcattca tcgcctcagc gcggttacgc
       61 tcctgggtcc ggtgctgctg gctggtgatc aggatgtcgc cgtccagcgt cagccgccgc
      121 cccgccaact tctccaaacg agctcgcacg ctgggcggca gggcagcccg gtccagcgac
      181 aggcgaagct gcaccgcggt ttcgaccttg ttgacgttct gcccgccagg acccgaggcc
      241 cggatgaagg tttcctgcac cgcgcgttca tcgatggata gcattctggt tatctggatc
      301 atgagggaac caacagaaac cttctacaac ataccaaccg ggccggtcgc ccacgtcgcc
      361 gtccgttcag ccggccaaac cacgagctag ccacaggtca gccggccagc cttgtccaag
      421 cggcgcggcc gaggaatact ccgcgcagtc aaaataacgc aggcatattg ccggctgacc
      481 agggaagctc cacgtcaatg aagacagcca taacgcgccg caaagccgtt ggacttggta
      541 tcgccgccgg aacgctgccg ctggtgaaca ttcactccgc cggcgcggcg gggcggctga
      601 gcgtggcgct gtgggaccat tgggtacccg ccggcaacca ggccatgaaa caaatggtcg
      661 gcgcctgggc cgaaaagaac aaggtcgatg tgacgttgga tttcctgacc tcggtcggca
      721 acaagatcac catcacgatg gcggccgagg cgcaggcgaa gaccggccac gacatctacg
      781 ccttcgacat gtggtcggtg catcaatatg ccgatcaact cgacccgatg gacgacatca
      841 tgcaggcgtt catcgcgaaa tacggcaaga tcggccgagc ctatgagtat ctgggagtgt
      901 cggaggggca ctggcgcgcg gtgccggtgg catggggatc ctcgccgctg accccgtgcg
      961 gccgcatcag catgctgaag caatacgccg acatcgacct gcaaaaatgg tttcccgcgc
     1021 atgaggcgac gcccgctacg tccgccgagt ggacctacga cgctcagctc aaagccgctg
     1081 ccgcctgcca caaggccggt ttcccgttcg gtttcggctg cgggacgacg accgactcca
     1141 accagacctg gggtgccatg ttcggcgcgt tcggagccga cctggtggac gccaagggcg
     1201 ccatcacggc cgattcggac aatgtccgcg cggcgcttga atacgcgaaa cggatggtgc
     1261 cgttcctgcc ctcggacacc gtcagctatg acgacgcctc caacaaccgg gcgttgattt
     1321 ccggcaggtc ggcgctgatc tggaacccgc cttcggcctg ggccgtggcc aagcgcgacg
     1381 ccccgcaggt tgcggcggac tgctggacgt tccccaaccc gcgcgggccc gccgggcgaa
     1441 tggtgccgat acggccgtat ttcttcggac tatggtcgtt cgcgcagaac aagccggcgg
     1501 cacgcgacct gctcgcgcat ctcggcgcgc gcgagcagat ggagacattg accaccgctg
     1561 tcgacggcta tgacatccca ccctacgcgt cgatggccga tttcaagatc tgggaagagg
     1621 tcggaccgcc gaagggcacg gtctataact atccgatccg cccctggcac gacgccgagt
     1681 attatgtcac tggatcctcc ggcccgccgg acatcggggt gcaggcgtgg aaccgcgggt
     1741 tgatcccgac gatggtgtcc aagatcgtcg ccggccagac catccagcag tcgatcgact
     1801 gggcgaagga cgagctggag ggattcacgc gttgattccg tcaccgcggg gtggccggga
     1861 cgagctgaag gcgatagacg atccggatat tgtgttgaac gcgtagatcg tcagccgaac
     1921 ccagggcggt ccggccgcaa cgatccgcgc cgctgagcgg attgccgatg accctgaccg
     1981 ccttgcttcg ccatctcggt ttcgccgcgc tgctcgtact gctgtccgcg gcaaccgtcc
     2041 gcatgatgat cgccgctcgg gtcatggaca cgccggaggc ccgcaaggcg cacgaccggc
     2101 caacgcccaa gggcggcggc gtcggcgtcg tcgtgacctt cctggtgggc ctggccgtgc
     2161 tttaccggta cgcggagttc gcccgcctcg ccaacccgta tttcctcggc gtcatcgagg
     2221 catcggtcgc gatcgcggtg gtcgccttcc tcgacgattt gttcgactgg ccgttcatcg
     2281 tcaagctggg cgcccaggtg ctggcggcgc ttgtcgccgt cggcagcggt ttgtacgtga
     2341 gcgatttccg cattccctat ttggggccac tggccttcgc atggatcggc gtcgccgcga
     2401 cggtgttctg ggtcctgttc accaccaacg cgctgaactt catcgacggc ctgaacggcc
     2461 tggccagcgg ggtggcgctg gtcgcctgtc ttttcattgt gttcatcgcc gaacagcatg
     2521 gcggctggtt cgcatacgcc gccgccgggc tgctggcagc cgggctggcc ggattcctgc
     2581 cgttcaattt ccccaaggcc cgtatcttca tgggcgatgt cggcagccag ttctgcggct
     2641 tcatgctggc ggtgctggcg gtcgtcgcca gccgcttcga cggcgtggaa ctgtctttcc
     2701 tgctaatgcc gatgctgttg tcgggcgtcc tgttcgatgt cgcgtttacc ttggtgcggc
     2761 ggacgctggc cggggagacc ctgacgcaac cgcaccgagg ccacctttat caggtggccc
     2821 agagggctgg cgtgtctccg gtggccgtca cgctggtgca ttggggcttc gcggtgttcg
     2881 gcggggtgtg ctgcctgctg ttcatcgccc tgccctcccc ctacaagccg ccggtcccgt
     2941 tcatcacggt ggtgccacaa ctttgttggg tgtggtttgt cgctgaccgg gcccatcgcg
     3001 ccgggttgag tcggtggggc taggaaggtc tgggatggaa tggcggccat gatcgccccg
     3061 cgatggaacg tttaaaataa acgatttagg ccgcggtctt catctcatgc ccgggcaacg
     3121 acaacccgcg tccgcgatgg ccgcgtcatc gcctccagtt gcagcaactc gttcagttgg
     3181 acctcggtca gcaagccctc atccaacacc acgtccgcca cggatcgacg ttccgccaac
     3241 gcccgcttgg cgacccgcga cgatgcctca tacccaagcg ccgggaccag ggccgtcacc
     3301 a
//
LOCUS       DASWQN010000773         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1129344, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000773 DASWQN010000000
VERSION     DASWQN010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129344"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VGC82_13060"
     CDS             <1..690
                     /locus_tag="VGC82_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY0424243.1"
                     /translation="EALVLLRSAGVNLDRVCRVEASTGCAAIAVDPAGENAIAVGSGA
                     NLFVRASQIEDTLLASAATLVLQMEVPALETALLIARARVAGTKVVLNLAPAANLPEA
                     ALRAVNVLVVNKTEAGWLAGHLGCGRTAIALRDRLGGIAVVLTRGAEGADVAGHDTEW
                     HEPAVAVEAVDTTAAGDCFVGVLAHRLDRGENLQSAVRRAALAAAVCCTRHGSQGSIP
                     TAAETDAFAME"
     gene            complement(743..1792)
                     /locus_tag="VGC82_13065"
     CDS             complement(743..1792)
                     /locus_tag="VGC82_13065"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0424244.1"
                     /translation="MPVRTFLAAVALLAASAQATHSAFAGPNDLTIVVRDESFLRALS
                     SAYVQPFTAITGTRVQQEVWEGGIETLRTQAKLPDNSWDLVLVDADELSAGCGEGLFE
                     KLDWSAIGAKEHYVAQAASDCGVGAIFATTVLAWDKDKLPVAPSWSDFWDVAKYPGKR
                     GLRKGVRGNLEFALMADGVAPADVYKTLSSSEGIDRAFRKLDQLKPYIVWWSAEADAA
                     HILALGDVLMTSAPSGQVATMAEHDHKRLGLQFTASLFELQSWAIIKSSPSARMAQQF
                     LYFTGMPAVELRLLQQSGDGGLAKGLNEGISPELAAVSPSNPANLAAGLKLDAGFWRD
                     NLPKLRQRFEAWQSH"
BASE COUNT          321 a          553 c          621 g          343 t
ORIGIN      
        1 gaggcattgg tgctgctgcg gagcgccggg gtaaatctgg atcgggtctg ccgggtggag
       61 gcaagtaccg gatgcgccgc aatcgccgtg gatccggcgg gcgagaacgc catcgccgtt
      121 ggctccggtg ccaacctgtt cgtgcgggcc tcccagatcg aggatacgct gctggcgtcc
      181 gccgccacgt tggtgctgca aatggaagtg cctgccctgg aaacggcttt gttgatcgct
      241 cgggcccggg tggctggcac aaaagtggtg ttgaacctgg cgccggcggc aaatttgccg
      301 gaagcggcgc tgcgggcggt gaatgtgctg gtggtgaaca aaacagaggc tggatggcta
      361 gctgggcacc tgggatgcgg ccggacagcc atcgctttgc gtgatcggct gggcgggatt
      421 gccgtggtgc tgacgcgtgg ggccgagggc gccgacgtgg cgggccacga tacagagtgg
      481 cacgaaccag ccgttgcggt cgaggcggtg gataccacgg ccgcaggcga ctgtttcgtc
      541 ggagtattgg ctcaccgctt ggatcggggc gagaacttgc aatcggcggt tcgccgggct
      601 gctctcgcgg cggcggtctg ctgcacgcgt cacggcagcc agggcagcat ccccacggcg
      661 gctgaaaccg acgcgttcgc tatggaatga ccacgcaatc cccatcccca tccccaactc
      721 cgttacttcc ggcgggaata gtctaatgcg attgccaggc ttcgaaacgt tggcgcagct
      781 tgggcaggtt gtcgcgccag aaaccggcgt ccagctttaa cccagccgcg aggttggctg
      841 gattgcttgg cgacacggct gccagttccg gggaaatccc ttcgttcagg cccttcgcca
      901 gaccgccgtc gccgctttgc tgcagcagcc gcagctcgac cgccggcatt ccggtgaaat
      961 acaagaactg ctgcgccatc cgggcggacg ggctgctttt gatgatggcc cagctttgca
     1021 gctcgaacag gctggcggtg aattgcaggc ccaggcgctt gtggtcgtgt tccgccattg
     1081 tcgcgacctg gccgcttggc gcgctggtca tcagcacgtc tcccagggcc aggatatgcg
     1141 cggcatcggc ctcggcggac caccagacga tgtatggttt gagctggtcc agcttgcgaa
     1201 aagcgcgatc tatcccctcg gagctggata atgtcttgta cacgtctgca ggggcgacac
     1261 cgtcggccat tagagcaaat tcgaggttgc cgcgtacgcc cttgcgcagg ccgcgttttc
     1321 ccggatattt cgcgacgtcc cagaaatccg accatgatgg cgcgaccggc agtttgtctt
     1381 tgtcccaggc caggacggtg gtggcgaaaa tcgcgcccac gccgcaatcg ctggccgcct
     1441 gcgccacgta gtgctccttg gcgccgatcg ccgaccagtc gagtttttcg aacaagccct
     1501 cgccacagcc ggcggacaat tcgtcggcat cgaccagcac caggtcccaa ctgttatcgg
     1561 gcaacttggc ctgggtgcgc agcgtttcga taccgccttc ccaaacttcc tgttgcaccc
     1621 gggtgccggt aatggcggtg aatggctgca cataagcgga gctcagcgcg cgcaagaacg
     1681 actcgtcacg aacgacgatc gttaaatcgt tggggccggc aaaggccgag tgagtggcct
     1741 gcgccgaggc cgcgagaagt gccacggcgg caaggaacgt gcggacgggc atgaacaggc
     1801 gaatttccgg gtttccaatg atggaccgac gctacagg
//
LOCUS       DASWQN010000774         6931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_470166, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000774 DASWQN010000000
VERSION     DASWQN010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470166"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(395..967)
                     /locus_tag="VGC82_13070"
     CDS             complement(395..967)
                     /locus_tag="VGC82_13070"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0424245.1"
                     /translation="MSGTLHQTMTVAAFLDWEDSQPLRYEFDGFRPCAMTGGSAGHSR
                     VQRNLIAALVSRLRGTSCEPFGSELKIMAANSVRYPDAFVVCSPVRDKATVVSDPVVV
                     FEIISPSTSGVDRIIKNQEYRDTPSIQRYVILEQDRQAATVFSRDHGNWAGHVMAGET
                     DLAMPEIGISIPLAELYTGVELAVDPPAEQ"
     gene            1115..1981
                     /locus_tag="VGC82_13075"
     CDS             1115..1981
                     /locus_tag="VGC82_13075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0424246.1"
                     /translation="MNDQPSDDPQTILDRIQQQAIRTETPCGEGKMVWHLWDQSGGTA
                     PVVVLFHGGAGSWRHWIRTIPALAPTYRVLVPDLPGLGESDFPPNGDDAFAIATIVAD
                     GIDRIIGTDTSYQVVGFSFGGTMAACIGALRGTRVRSVTIIGSSGVGSLGSAVKLEKV
                     RHLEGQERRDTHRTNLNRLMIADPAKIDDLAILIQDWNTVRSRLRTPAISRSGVIMKA
                     IDQLQSPLNGMWGELDAPANPKGPERVATLRAHCPDADIRLIPKTGHWAAYEAADTVN
                     AILLEMLARTRT"
     gene            2013..4628
                     /locus_tag="VGC82_13080"
     CDS             2013..4628
                     /locus_tag="VGC82_13080"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0424247.1"
                     /translation="MNEASTGSLSKGPGEMMGSAGPGMLAGLRVIEIADERAEYTGLL
                     LAGLGCDVVKVEPPEGNATRRIGPFLDDTPGQERSLFFWNYNRGKKSVVLDIQSQPDS
                     LLRLLDGADILLDSSCGALNTALNLDRAALNARFPKLITARMTPFGDDGPWANFKASD
                     LIHLALGGVMMNCGYDPDPNLQYDSPPIAPQVWHAYHIAGEQLATGIIAALIARHRTG
                     EGQDVSVAVHEAVSKNPELDIMHWVMRRVPLWRLTCRHALETPNHSPSIMHTKDGRWY
                     ISHGMGARDLKALVPLLSKYGMQADLQPPPPDADLKARAVPGSTASDEARAHMIDVVQ
                     RFTRAWTYQDMPWLEAQEAGLLWAPIRKPHENALDEHWLQRKSFANVYHPEQQRSFRY
                     PTSKWLATKTSWQVGRRAPLLGEDTETVLAEQPRLSSVPARPKGDGANPAVSALHGKP
                     FPLQDVKILDFAWFLASAGGTRFLSAMGAQSYKVEWKDNPDTRLAAMAPVGGRAARDA
                     ATGPLPGVKDPDMGGQFNNKNAGKRGISLNIRHPKGLAIAKDLVRICDIVAEGFSPGV
                     LERLGLGYDVMRKIRPDIIYIQQAGMGAHGTYGRMRTVGPVAAAFGGQADMSGLPEPA
                     MPVGWGYSYLDWMGAYGYALALLGALYHRDRTGEGQWIDASQCESGLFLTGTSILDWS
                     ANGREWRRYGNRSPYKPAAPHGAFRARGKDRWVAIACFTEAEWQALARAAGHLEWLDD
                     KRFATLADRLAHQDALESIIGAWTATNSAEDVMTVLQNASVPAGVCQNAEDRCDNDPQ
                     LRHLKWLTEVTGTKIGTWPVYELPMKFSRTPAYIGGQIDRGAPGYGEDNEWLLTTMLG
                     MTPSDVQRLAEEGVI"
     gene            complement(4788..5318)
                     /locus_tag="VGC82_13085"
     CDS             complement(4788..5318)
                     /locus_tag="VGC82_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424248.1"
                     /translation="MFEKAGITGDLEVQGQGGNLIPVVTLRGLPSEMLMAASAAGNTE
                     ASIQLPGSPREDLNCAATGTGYICAPNAAGGPRLSAGLAAAAAITIRVSVSMAGVGKL
                     PAQEKVLELADTNAALARLRRLGPSQVPEGAASLAPPSTSGMMAMADAALKAAGYKNG
                     LTDVQALLAKIMKGRQ"
     gene            5532..6560
                     /locus_tag="VGC82_13090"
     CDS             5532..6560
                     /locus_tag="VGC82_13090"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY0424249.1"
                     /translation="MNDALQPDPLSIHRTIVTLDTHIDIPWPTGPDPFQDGPRRVDLP
                     KMLRGGLSAACFVAYVPQTSRTVASEDAAFNRAIAMLEQINAMEQMPSAIPMRIANRA
                     DAIEAAKRDDVLAIVPCVENGFAIGADLGRIAELRQRGAIYMTLTHNGHNALADSCNP
                     RRDLGDRETEHGGLSALGRAAVAAMNKAGMLVDVAHTSRDTMVQAAEASRSPIVSTHS
                     SISAICDHSRNMTDWQLDVIRDVKGVIQITAVAAFLKPNAKPEEVTVADFADHIDYAV
                     KRIGIDHVGISSDFDGGGGFSDWHDASQSPNITIELVRRGYDRGALEKLWGGNFLRVM
                     RVAEGMAS"
     gene            6560..>6931
                     /locus_tag="VGC82_13095"
     CDS             6560..>6931
                     /locus_tag="VGC82_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0424250.1"
                     /translation="MRTVALPGGQTVPAIGQGTWYMGERRTDTQREADALRLGIDLGM
                     TLIDTAEMYASGGAEEVVAQAVSGIRDRVFIVSKVLPQNASRSGVAAACDRSLKRLRT
                     DRIDLYLLHWRGGHPLAETVAA"
BASE COUNT         1289 a         2208 c         2198 g         1236 t
ORIGIN      
        1 atatgggaat tcatgaactg ggtctggatc cggcggattt gctgccgccg ctgaagccgc
       61 tttaactttt ctgagcgtga tcacgctggg cggaacgtcc gtcgtccaaa ggacgcgctg
      121 ttcgcgatga gtatgagtca cgcgaaggac gcgctgttcg cgtatgagtc acgtgatcaa
      181 acaaggagat agaccattcg cagcctgcac agtaccgtcc gaagagtcgg aatcaacgtg
      241 agttggtatc acgccccaac gatcggttga tcaggtcgga cagatggttc ttggcgtcgg
      301 ctacgctgtg ctggctcatg gtcctaatat cgcaatctga tggccatttg cccagcgcga
      361 tcactgcagc ggcgtcgata ctctccgcaa cactttactg ctcggccggc ggatcgactg
      421 ccagttccac cccggtataa agttctgcca agggaatcga aataccgatc tccggcatcg
      481 cgagatccgt ttcacccgcc atcacatgcc cagcccagtt cccgtggtcg cggctgaaaa
      541 cagtggctgc ctggcggtcc tgctccagga tcacgtaccg ctgtatcgac ggcgtgtccc
      601 gatactcctg gttcttgatg atgcggtcca caccagaggt actcgggctg atgatctcga
      661 acaccactac cggatcgctg acgacggttg ccttgtcgcg gacgggcgag cacaccacga
      721 aggcatccgg atagcggacg ctattcgccg ccatgatttt caattcgctg ccaaatggct
      781 cacaggatgt tccgcgcaag cgggacacca gggccgcgat caggtttcgt tgcacgcgag
      841 aatgaccagc cgaaccgccc gtcatcgcgc atgggcgaaa accgtcgaat tcataacgga
      901 gcggctggct gtcctcccaa tccaggaaag ccgctaccgt catcgtttgg tgcagggtgc
      961 cgctcatcgc ttgaacatag cacgtcggcg gtcgttcggc agctcgattt ggcgacactc
     1021 gtttggcgtc ttctcgattg gcgtccctcg atttggcgtc cggccgcggc tgtctctaaa
     1081 ctaacccgcg accgtaccag aggacccatc cgggatgaac gaccagccta gcgacgatcc
     1141 ccagaccatc ctggaccgca tccagcagca agccattcgc accgaaacgc cctgcggcga
     1201 gggcaagatg gtctggcacc tgtgggacca gtctggcggc accgctccgg tcgtcgtcct
     1261 gttccatggc ggagccggat cctggcggca ctggattcgt accatcccgg ccctggcccc
     1321 gacataccgc gtgctggtcc ccgacctccc tggtctcggc gaatcggact ttcctcccaa
     1381 cggagacgat gccttcgcca tcgcgacgat tgtcgccgac gggatcgaca gaatcatcgg
     1441 caccgacaca tcataccagg ttgtcggctt ctccttcggc ggcaccatgg ccgcctgtat
     1501 cggtgcgctt cgtggcacgc gggttcgctc cgtcaccatc atcgggtcgt ccggcgtcgg
     1561 ctctctcggc tccgcggtga agctcgaaaa ggtccgccat ctggaagggc aggagcggcg
     1621 cgacacccac cgcaccaacc tcaatcgcct gatgatcgcc gacccggcga agatcgacga
     1681 tctggcgatc ctgatccagg actggaacac cgtccgctcg cgcctccgca cgccggccat
     1741 ttcccgcagc ggcgtcatca tgaaggcgat cgaccagttg cagtcgccgc tgaacggcat
     1801 gtggggcgag ctcgatgctc cggccaatcc aaagggaccg gagcgcgtcg caaccctgcg
     1861 cgcgcattgc ccggacgccg atatccggct gatcccgaaa accggccatt gggcggccta
     1921 tgaagccgcc gataccgtga acgcaattct gctggaaatg ctggcgcgca cacgcacctg
     1981 accacaggct cgcggggggg aggaagcaaa caatgaacga agcgtccaca ggttccttgt
     2041 caaaaggtcc gggtgagatg atgggcagcg ccggccccgg catgctggcg ggcctgcggg
     2101 tcatcgagat cgccgacgaa cgcgccgaat acaccggcct gttgcttgcc ggcctcggct
     2161 gcgacgtcgt gaaggtcgaa ccgccggagg gcaacgcaac ccgccgcatc ggcccgttcc
     2221 tggacgacac gccaggccag gaacggtcgc tgttcttctg gaactacaat cgcggcaaga
     2281 aatccgttgt cctggacatc cagtcgcaac ccgattcgct gttgcggttg ctcgacggcg
     2341 ccgacatcct gctggacagt tcatgcggtg ccctgaacac cgcgctgaac ctggatcgag
     2401 ccgcgctcaa tgcgcgtttt ccgaagctca tcaccgcgcg catgaccccg ttcggcgacg
     2461 atggaccgtg ggcgaacttc aaggcgtccg acctgattca tctggccctg ggcggagtca
     2521 tgatgaattg cggctacgac ccggatccga acctgcaata cgactcgccg ccaatcgcac
     2581 cacaggtgtg gcacgcctat catatcgccg gcgagcagct tgccaccggc ataatcgccg
     2641 cgctgatcgc ccgccatcgc accggcgagg gccaggacgt ttcggttgcc gtgcatgaag
     2701 cggtgtcgaa gaatcccgaa ctcgatatca tgcactgggt catgcggcgg gttccgctgt
     2761 ggcgcctgac gtgccggcac gcgctggaaa cgccaaatca ttcgccgagc atcatgcata
     2821 ccaaggacgg ccgctggtat atctcgcacg gcatgggcgc gcgcgacctg aaggccctgg
     2881 ttccgttgct gtcgaagtat ggcatgcagg ccgacctgca accgccgccg ccggacgccg
     2941 acctgaaggc gcgtgcggtg ccgggctcga ccgcgagcga cgaagcccgg gcgcacatga
     3001 tcgacgtggt gcagcgcttt acccgcgcct ggacgtacca agatatgccc tggctggagg
     3061 cgcaggaggc tgggctgctg tgggcgccga tccgcaagcc gcacgagaac gcgctcgacg
     3121 agcactggct gcaacgcaaa tccttcgcca atgtgtacca tccggagcag cagcgctcat
     3181 tccgctaccc caccagcaaa tggctggcga cgaaaacaag ctggcaggtc ggccgccgcg
     3241 cgccgctgct tggcgaggat accgaaacgg tcctggctga gcagccacgg ttgtcctcgg
     3301 tcccagcgcg gccgaagggc gacggcgcca atccggccgt ctcggcactg cacggcaagc
     3361 cgtttcccct gcaggacgtg aaaatcctcg attttgcatg gttcctggcg tccgccggag
     3421 gcacgcgttt cctttccgcc atgggtgcgc aaagctacaa ggtcgaatgg aaagacaacc
     3481 ccgacacccg cctcgccgcc atggcacctg tgggtggccg agcggcgcgc gatgccgcca
     3541 ctggcccgct gccgggtgtg aaagatccgg atatgggcgg ccagttcaac aacaagaacg
     3601 ccggcaaacg agggatttcg ctgaacatcc gtcatcccaa gggcttggcg attgccaagg
     3661 acctcgtgcg gatctgcgat atcgtcgccg aggggttttc accgggggtg ctggaacgcc
     3721 ttggcctcgg ttacgacgtg atgaggaaaa tccggccgga catcatttac atccagcaag
     3781 ccggcatggg cgcacacggc acttacggcc ggatgcgcac tgttgggccg gtcgccgcgg
     3841 cgttcggcgg ccaggctgat atgtcgggcc tgcctgaacc ggcgatgccg gtagggtggg
     3901 gatactccta cctcgactgg atgggcgcct atggctacgc gcttgccctg cttggcgccc
     3961 tgtatcaccg cgaccgtacg ggagagggcc agtggatcga cgcctcgcag tgtgagtccg
     4021 gattgttcct gaccgggacc tcgattctgg actggtcggc caacggccgg gaatggcgcc
     4081 gctacggcaa ccgctcgccg tacaaaccgg ccgccccgca tggcgccttc cgtgcccggg
     4141 ggaaggaccg gtgggtcgcc atcgcctgct tcaccgaggc agagtggcag gcattggcgc
     4201 gcgcggcagg tcacctggaa tggctggacg acaagcgttt tgccaccctg gcggatcgtc
     4261 tcgcgcatca ggacgccttg gaatccatta tcggcgcctg gaccgcgacg aacagcgcgg
     4321 aggacgtgat gacggtcctg caaaatgcct cggttcccgc cggagtctgc cagaacgccg
     4381 aggaccgttg cgacaacgac ccgcagctgc ggcacctgaa atggctgacc gaggtcaccg
     4441 gaaccaagat tgggacctgg ccggtgtacg agttgccgat gaagttctcc cgcaccccag
     4501 cctatatcgg cggccaaatc gaccgcggcg cacccggcta cggcgaggac aacgaatggc
     4561 tgctaacgac catgcttggc atgacgccgt ccgacgtgca gcgcctggcg gaggagggag
     4621 tcatataaag gcgagtttac ctgcttctcc tggtgtcctg cccccggatg ttcgtcgcgt
     4681 catgcggtgg acgcagggga tcagcaagcc actgaaacag agaaagggtg gcgcgagact
     4741 gaggtcccct aaggcggact tcagattcgg gacaccagac caggctttta ttgacgcccc
     4801 ttcatgattt tcgccaacag tgcctgaacg tcagtcaatc cgttcttgta acccgccgcc
     4861 ttcaacgccg cgtcggccat tgccatcatc ccgctggtgg acggcggcgc cagtgaggct
     4921 gcgccctccg gcacctgcga cggaccaagt cttcgcagcc tggccaaagc agcattcgta
     4981 tcggccagct ccaggacttt ctcctgagca ggcagtttgc cgacgcccgc catggaaaca
     5041 gacactctga tggtaattgc cgccgccgcc gccaaaccgg cagacaatct tggcccgccg
     5101 gcggcattcg gcgcgcagat gtagccggtc ccggtggcgg cgcagttcag atcctcacgc
     5161 ggacttcccg gcagttggat ggatgcttcg gtgttgccgg ccgccgaggc tgccatcagc
     5221 atctcgctgg gcaggccgcg cagcgtgacg accgggatca gattgccgcc ttgaccctga
     5281 acctcaaggt cgccggtgat gcccgccttt tcgaacaagc gctttttcgc acgcatcaga
     5341 caccggtcag ggcgtggctt atctccgggg cagaccaata cccaactgcc gatttgctcc
     5401 tggctcgcgg cccgcaagcc ggtggttatc gtgacgaatc ccatggtcac gagggcgctc
     5461 cagccggctg cccgcctcat tttgcacgcg aacctcttcc gtaccgcgca attatctgta
     5521 agcgtgtcgg gatgaacgat gccctgcagc cagacccgct ttcgatccat cgaaccatcg
     5581 tgacgctgga tacgcatatc gacattccct ggcccactgg tccggacccg ttccaagatg
     5641 gcccccgccg cgtagatttg ccaaaaatgc tgcgtggcgg gctcagcgcg gcctgctttg
     5701 tggcctatgt gccgcagacc tcgcggaccg tggcatccga ggatgcggcg ttcaaccgag
     5761 ccatcgcgat gctggagcag atcaacgcga tggagcagat gccgtcggcc atccccatgc
     5821 gcatcgccaa tcgggcggac gcaatcgagg cagccaagcg ggacgacgtc ctggcgatcg
     5881 tgccttgcgt ggagaacgga ttcgccattg gggccgattt gggccggatc gccgaactgc
     5941 ggcagcgcgg ggcgatctac atgacgctga cgcataacgg gcacaatgca ctggcggatt
     6001 cctgcaatcc gcgacgggac cttggggacc gggaaaccga gcacggcggg ttgtcggcgc
     6061 tgggacgggc agcggtggca gcgatgaaca aggctggcat gttggtggat gtggctcaca
     6121 cgtcgcgcga cacgatggtg caggcggccg aggcgtcccg ctcgccgatc gtttcgaccc
     6181 actccagcat cagtgcgatc tgcgaccatt cccggaacat gacggattgg cagttggacg
     6241 tcattcggga cgtgaagggg gtcattcaga ttaccgccgt cgcggcgttc ctgaagccca
     6301 acgcaaagcc cgaggaggtg acggtggccg acttcgcgga tcatatcgac tacgcggtga
     6361 agcgcatcgg gatcgatcat gtcgggatat cctccgactt cgatggcgga ggcgggtttt
     6421 ccgactggca tgacgccagc cagagcccga acatcacgat cgagctggtg cggcgcggct
     6481 atgaccgggg cgcccttgaa aagctgtggg gcggtaattt tctgcgggtg atgcgggtgg
     6541 cggaggggat ggcgagctga tgcgaacagt tgcattgccc ggcgggcaga cggttccggc
     6601 gatcgggcag gggacctggt acatgggcga gcgccggacc gacacccagc gagaggccga
     6661 cgccttacgg ctcggtatcg atcttggcat gacgttgatc gacacggccg agatgtacgc
     6721 cagcggaggg gcggaagagg tcgtcgccca ggcggtttcc ggcatcaggg atcgcgtttt
     6781 catcgtcagc aaggtgctgc cgcagaatgc ctcccgatcc ggggtggcgg cggcgtgcga
     6841 tcgcagcctg aagcggctgc ggaccgatcg gatcgacttg tatctgttgc actggcgtgg
     6901 cgggcatcct ctggcggaga cagtcgccgc g
//
LOCUS       DASWQN010000775         1829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_206887, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000775 DASWQN010000000
VERSION     DASWQN010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206887"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VGC82_13100"
     CDS             complement(<1..819)
                     /locus_tag="VGC82_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0424251.1"
                     /translation="MLIFVVLSLPARAQERIVNIYNWTDYIDPSVLVDFTKETGIKTR
                     YDVFDSLETLEAKLLAGHSGYDIVAPSNEPSFSRLIRAGALATIDRAKVPNWANLDPA
                     LMQRVEPSDAGNRHGPIYLWGSIGLGINPGRIQALAPDAPLDSWALLLDPAWAKQVAP
                     CGIIMMDSAIDVIPSVLRYLGRSPDSSTPEDLNAVLKALMAIRPFIRDFASGGALEAL
                     ATGQACLALDYSGDVAQASARAKAGVGLRYVAPKEGAEIGFDMLAIPSDSRHKAE"
     gene            complement(831..1628)
                     /locus_tag="VGC82_13105"
     CDS             complement(831..1628)
                     /locus_tag="VGC82_13105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0424252.1"
                     /translation="MSSAAPGRIAAVTGAASGIGEAACRRLLQAGWTVFGLDVAERRL
                     DAVRDNFAEFQTRFRPVVCDVSDAGSVTPAFAGIDKLNALVCCAGVLRTSLLEDMAVE
                     DFDLVLNTNLRGTFLCAQKALPALRRTATVENPFRVVLLSSVAALRPKIVSGAYAASK
                     AAVSQLCRVMAAEWAPSGVLVNALAPGTVDTPMVRAVSDPAASKGYRPSGVSPVGRIA
                     QPDDVVDVMMFLLSDAARYVTGTTIPVDGGTQAAFIPPGSSLPERLF"
BASE COUNT          357 a          612 c          554 g          306 t
ORIGIN      
        1 ctcggctttg tggcgcgagt cggacgggat cgccagcatg tcgaacccga tttcggcgcc
       61 ttccttcggt gccacatagc gcaaaccgac tcccgccttg gctcgggcgg aggcctgcgc
      121 cacatcgccg gagtaatcca gcgccaggca ggcttgtccc gtcgccaagg cctccaacgc
      181 gccgccactg gcaaaatcgc gaataaaggg acgaatcgcc atcagggctt tcaggaccgc
      241 attcagatct tccggcgtcg aactgtccgg cgagcgacca aggtagcgca gcaccgatgg
      301 gatcacgtcg atcgccgaat ccatcatgat gatcccgcag ggcgcgacct gtttcgccca
      361 ggccggatcg agcagcagcg cccatgaatc caatggcgcg tcgggcgcca gcgcctggat
      421 gcgcccggga ttgataccta atccgatcga cccccacaga taaatcggcc catgccggtt
      481 gccggcatcg gacggttcga cacgctgcat cagcgctgga tccaggttcg cccagttcgg
      541 caccttcgcc cggtcgatgg tcgccagcgc cccggccctg atcaaccgcg aaaaactcgg
      601 ctcgttgctc ggtgcgacga tgtcatagcc ggaatgcccg gccagcagct ttgcttccag
      661 cgtctcaagg ctgtcgaaca catcgtagcg ggtcttgatc ccggtttcct tggtgaagtc
      721 caccagcacg gacgggtcga tgtagtcggt ccagttatag atgttgacaa tgcgttcctg
      781 cgcccgggcc ggcagcgaca ggaccacgaa gatcagcaac cagcgcatcc tcagaacagc
      841 ctttccggga gcgacgagcc aggcgggatg aacgccgcct gcgtgccgcc atcgaccgga
      901 atagtcgtgc cggtcacgta gcgcgcggcg tccgacagca gaaacatcat cacgtccacc
      961 acatcgtccg gttgggcaat ccgcccaacg ggggagacgc cggacggccg ataccctttg
     1021 gacgccgccg gatcggaaac cgcccgaacc ataggtgtat ccacggtccc gggtgcgagg
     1081 gcgttgacca gcacgcccga cggcgcccac tccgccgcca tcacccggca aagctggctg
     1141 accgcggcct tggatgcggc ataggctccg ctgacgatct tcggccgcag cgccgcgacg
     1201 gatgacagca gcaccacccg gaatggattt tcgacagtcg ccgtccgccg cagtgccgga
     1261 agggcctttt gcgcgcacag gaaggtgccg cgcaggttgg tgttcaggac aagatcgaaa
     1321 tcctcgacag ccatgtcctc cagcagcgat gtccggagga ccccggcaca gcaaaccaac
     1381 gcgttcaatt tgtcgatgcc ggcgaacgct ggcgtcacac tgcctgcatc cgagacatcg
     1441 cataccaccg gccggaaccg ggtctggaat tcggcgaaat tgtcgcgcac cgcatcgagc
     1501 cggcgttcgg ccacgtccag tccgaagacc gtccagccag cctgcagcag tcgccggcat
     1561 gccgcttcgc caatccctga ggcggcgccg gtaactgccg cgattcttcc cggtgctgcc
     1621 gagctcatga tggttcctcc gcgatgcgtg atgcgatgct acaatgtgaa ccggcggaac
     1681 gcggcatcat agaacttgaa gtggccagcc aatgccagac ggcacatgcc agatgggcac
     1741 aggtatccgc gaggttccat aagcggcggc cggggcgctg ctgtctgtca cgaatttacc
     1801 ctgatgatgg cttcataatg ccggattcc
//
LOCUS       DASWQN010000776         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2582620, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000776 DASWQN010000000
VERSION     DASWQN010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582620"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /gene="pgi"
                     /locus_tag="VGC82_13110"
     CDS             <1..1143
                     /gene="pgi"
                     /locus_tag="VGC82_13110"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY0424253.1"
                     /translation="MAYRALRPFSDRSMTFRFVANVDGADFHEATIGLDAAETLFIVS
                     SKTFTTLETMTNAATARAWLVAKLGSDAAVAKHFAAVSTNIPAVTTFGIDQANTFGFW
                     DWVGGRYSMDSAIGLSTMIAVGPENFRAMLAGFHAMDEHFRTAPPARNLPLLLGLLTV
                     WYNNFFGAQTLGIMPYSAHLARFPAYLQQLQMESNGKHVDFDGQVVDYQTGPIIWGEP
                     GTDSQHSFYQLLHQGTKLVPCDMIGFCKPLSPLTEQHDLLMANLFAQAEALGFGKTAQ
                     ELKAEGSPDFQTPFRVTEGNRPTNMILAERLDPATLGSLVALYEHSVFTQGAIWAIDS
                     YDQWGVELGKVLASRIIPELDRGTDPKLAHDASTNALISRYRRLRG"
     gene            complement(1144..>1555)
                     /locus_tag="VGC82_13115"
     CDS             complement(1144..>1555)
                     /locus_tag="VGC82_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0424254.1"
                     /translation="AARFTRVLGTLLVNGVSLITALGVVSDALGNQAAVAAVEDATTS
                     ARGGGGLAEPLSRSRIFPTRAIHLLRLGEENAQLGPMALRAADIHEERTRLALQRMVA
                     LLVPAITILMGLAVAGIVASLMTAMLSLNDLAGA"
BASE COUNT          286 a          499 c          497 g          273 t
ORIGIN      
        1 atggcatatc gggcactgcg cccgttcagc gaccggtcga tgacgttccg gtttgtcgcc
       61 aacgtggacg gcgcggattt ccatgaggcg acgatcggcc tggacgcggc ggagacgctg
      121 ttcatcgtct cctccaagac cttcacaaca ctggagacga tgaccaacgc ggcaacggcc
      181 cgcgcgtggc tggtcgcgaa actaggatcg gatgccgcgg tggcgaagca tttcgccgca
      241 gtttccacca acattccggc cgtcacgacg ttcggcatcg accaggccaa tacgttcggc
      301 ttctgggact gggttggcgg gcgctattcg atggacagcg ccatcggctt gtccacgatg
      361 atcgcggtag ggccggagaa tttccgcgcc atgctggccg ggtttcatgc gatggacgag
      421 catttccgca ctgctccccc tgcccgcaac ctgccgttac tgcttggact gctgaccgtc
      481 tggtacaaca acttctttgg cgcccagact ttggggatca tgccctattc ggcgcatctg
      541 gcgcggtttc ccgcctatct ccagcagctt cagatggaaa gcaacggcaa gcacgtggat
      601 tttgacggac aggtcgtgga ctatcagact ggcccgatca tttggggtga accagggacc
      661 gacagtcagc actcgttcta ccaactgctg caccagggca cgaagctggt gccctgcgat
      721 atgatcgggt tctgcaagcc gctcagtcca ttgaccgaac agcacgacct gctgatggcg
      781 aacctgttcg cccaggccga ggcgctgggc ttcggcaaga ccgcacagga gctgaaggcg
      841 gaggggtcac cggatttcca gacgccgttc cgcgttaccg agggcaaccg gccaaccaac
      901 atgatcctgg cggaacgcct ggaccccgca accctcggga gcctggtggc gctgtatgag
      961 cacagcgtgt tcactcaagg ggccatctgg gcgatcgaca gttatgacca gtggggcgtc
     1021 gagctgggca aggtgctggc cagccgcatc atccccgaac tggaccgcgg caccgacccg
     1081 aagctggcgc acgatgcttc caccaacgcg ctgatcagcc gctaccgtcg tttgcgggga
     1141 taatcaggcg ccggcgagat cgttcaggct gagcatcgcc gtcatgaggg acgcgacgat
     1201 tccggcgacc gcgaggccca tcaggatggt gatcgccgga accaagaggg cgaccatccg
     1261 ctgcaaagcc aggcgggtgc gttcctcgtg gatgtcggcc gcgcgcaggg ccattggccc
     1321 caactgggcg ttttcctctc ccagtcgcag cagatggatg gcgcgggtcg gaaagatgcg
     1381 gctgcgcgac agcggttcgg ccagaccgcc gccaccacgg gcgcttgtgg tcgcatcctc
     1441 gaccgcggcc accgcggcct ggttgcccaa cgcgtcgctc accaccccga gcgcggtgat
     1501 caacgacacc ccgttgacga gcagcgtgcc caggacgcgc gtaaacctgg cggcg
//
LOCUS       DASWQN010000777         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2054604, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000777 DASWQN010000000
VERSION     DASWQN010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054604"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VGC82_13120"
     CDS             complement(<1..417)
                     /locus_tag="VGC82_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424255.1"
                     /translation="MDADEQIRVRQGANPATNPTVLRQLAHDPSVTVRASLALNPALP
                     EQVVAILAADTDARVRTILGRKLATLAPTLTNETRHQVQQDAVARLTAMVAEAGQRVR
                     ANISEAVRAMPDGPREIILRLAHDPAVMVCEPIIRFS"
     gene            complement(417..1820)
                     /locus_tag="VGC82_13125"
     CDS             complement(417..1820)
                     /locus_tag="VGC82_13125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0424256.1"
                     /translation="MTELILRPPVTASPSAPPSQDLIRDALVESRQRWRHLVTLAADL
                     AFETDSKGRFVFVIPEDGLGWPAGSLIGQPSEVLLGDDGTGSVFNAFRPVVEVRRHRI
                     WLRCFDGGLAMMTVSAAPLYDRHGNIVGARGTAIDTTDGDTQTSRIAGRLRRGEALDY
                     ILARVAQETGADSMMDAALWALIHALGAEGSAVIGSLAEDAAAEVLHECGPGASAILP
                     VAANLVQHGIEPAHATNQDGRLVVAVGCRTRFGVNSGLAVWRGAAGLAWDPEDLLLAA
                     SAGNIVRMILEYEAVQRDMAQQARTDSLTGLLNRRAFLEEVRRHLERLDRETEAGTLM
                     FVDLDAFKAVNDRLGHAMGDQVLVHVADMMRGLVRPSDLIARLGGDEFAVWLSGADHM
                     TAAERADQLCKQAPVEIQDMLPEAFAGLGVSVGIATRRRGSAESIEDLMRRADMAMYE
                     VKRNGRRHWRVSLLDGD"
BASE COUNT          331 a          662 c          646 g          330 t
ORIGIN      
        1 cgaaaaccgg atgatcggct cgcacaccat cacggcagga tcgtgtgcca gccgcaggat
       61 gatctcacgc gggccgtcgg gcatcgcacg caccgcttcg gagatattgg cccgcacccg
      121 ctgccccgct tcggcgacca tcgccgttaa cctggcgacg gcgtcctgct gtacttggtg
      181 acgcgtctcg ttcgttagcg tcggggccag ggtcgccagc ttgcggccca ggatcgtccg
      241 tacccgggcg tcggtatcgg ccgccagtat cgccacgacc tgttcgggca gtgccgggtt
      301 cagtgccagg ctggcacgca cggtgaccga gggatcatgc gccagttgcc gcaacacggt
      361 cggattggtg gccggattcg cgccctggcg gacgcgaatt tgctcgtcgg cgtccatcag
      421 tcgccgtcca gcagggaaac gcgccagtgg cggcgtccgt tgcgcttgac ttcatacatc
      481 gccatgtcgg ctcgcctcat gaggtcctcg atcgattccg cgcttccccg ccggcgtgtc
      541 gcgatcccga ccgacacccc caggcccgcg aacgcttcgg gcagcatgtc ctggatttcg
      601 acgggcgcct gtttacacag ttgatcggca cgttccgcgg cggtcatgtg gtcggcaccg
      661 ctcaaccaga cggcgaactc atcgccgccc aggcgagcaa tcaggtcgga cggccgcacc
      721 agcccgcgca tcatgtccgc tacgtgcacc agcacctgat cgcccatagc gtggccaaga
      781 cgatcgttga ccgccttgaa cgcatccagg tccacgaaca tcaacgtgcc ggcctccgtc
      841 tctcggtcga gacgttcgag gtgacgccgc acctcctcaa ggaaggcacg cctgttgagc
      901 aaaccggtca gcgagtcggt gcgcgcctgt tgcgccatgt cccgttggac ggcttcgtat
      961 tccaggatca tgcgcacgat gttgccggcc gatgctgcca gcaacaggtc ctccggatcc
     1021 caggccaggc cggcggcgcc gcgccagacc gccagcccgg aattgacgcc gaagcgggtg
     1081 cggcagccga ccgccaccac gagtcgcccg tcctggttcg tggcatgagc aggttcgatg
     1141 ccgtgttgga ccagatttgc cgcgactgga aggatcgcgg aagcgcctgg gccgcactcg
     1201 tgcagcactt cggctgcggc gtcttccgcc agactgccaa tcacggccga accctcggcg
     1261 cccagcgcgt ggatcaacgc ccacagggcg gcgtccatca tgctgtcggc gccggtttcc
     1321 tgtgcgacgc gggcaaggat gtaatccaga gcttcgccgc gccgcagccg gccggcgatc
     1381 cgcgaggtct gggtatcgcc atcggttgtg tcgatcgcgg tgccccgcgc gccgacgatg
     1441 ttgccgtgcc ggtcgtacag cggtgcggcg gaaaccgtca tcatcgcgag gccaccgtcg
     1501 aagcagcgaa gccagatgcg gtgtcgccga acctccacga ccgggcggaa ggcgttgaaa
     1561 acggagccgg tgccgtcgtc gccgaggaga acctccgagg gctggccgat cagcgatccg
     1621 gcgggccagc caagcccatc ctcgggtatg acgaacacga agcggccctt ggaatcggtt
     1681 tcgaacgcca gatcggccgc cagggtgacg aggtgccgcc agcgctgacg gctttcgacc
     1741 agcgcatccc gaataaggtc ttgcgacggt ggcgccgatg gcgaggccgt aacaggtggg
     1801 cggaggatca gttcggtcat gggcggcgag tcccgaggag gctggacgcc atcaagccac
     1861 gaataggtta accaaaggtt gccgccgccg gtttgtgctc tgtcggcaag tacccgcttc
     1921 aaacgcgatc tcgacgccgt cgtagtgaac gaagtctgca gcgttcgat
//
LOCUS       DASWQN010000778         5027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1922794, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000778 DASWQN010000000
VERSION     DASWQN010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922794"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="VGC82_13130"
     CDS             complement(<1..1537)
                     /locus_tag="VGC82_13130"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0424257.1"
                     /translation="MRRSIRGKLLGSFAVVVLLASTSGVVGLWALDHATTVTNGVLAE
                     EVPDLEAGLAAAQVLQRSIADLQRYSRSLDGLGQLRGSIDNDLSRFNSLMLSIRDGHA
                     DVASRAGEEPHGAIAATGRVKELVDATLTEHAVLSTAATAAIAAHDRRAAYIFDLDGR
                     RLDLASFTSLQAVQLSRWISDLGEAVRFETPFKGGTDAGKSTFARWQAGFKAPDDKLQ
                     KMLKDFAAANVQIHEAAASIMAADGETRASDLERNRVRLFVKAQRQAEALVDYAGPLL
                     LGLDQAEAQALAQLETVAKRIDARLGELTKELRSTFVNAQHEATSSSQIAWMIAAVTL
                     AAGFIGSFALALMAGRAISRPIENLTVVTRRLSEGELDVEIPGAQRKDEIGALAVAVE
                     TFKLGSIERQRLEAEQRTEQAKKEQRAKMVDGLIARFETQVTGALQTLASSAGDLNST
                     ASRMAETAEETTRQVTNVAAASEQATSNVQTVAAATEELSASVSEIGRQMEQSTRIAS
                     QAVA"
     gene            complement(1560..2078)
                     /locus_tag="VGC82_13135"
     CDS             complement(1560..2078)
                     /locus_tag="VGC82_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424258.1"
                     /translation="MKQSFRAFALVAVLAAPVAAAQAMDTVAYKTQVEETIREVISGN
                     FPSIDKTLARQDEIIKIGVEGAREFAVDKPEYAKLMQLTIANVDTMKKMEPEQLEAQW
                     GDGAEAFKAAGFDITKLSQFSAARSHADTIIHPTTSFVLLSLYKKTGDKALLEQAKSE
                     LVEVLEHLQHLN"
     gene            2261..2707
                     /locus_tag="VGC82_13140"
     CDS             2261..2707
                     /locus_tag="VGC82_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424259.1"
                     /translation="MREEAQLRERLRKIEALFASAGTAGERLAAEAALDRVGSRLAAM
                     NERDPAVEMQFSMPDQWSRRLFLALSRRYGLQPFRYRRQRLTTVVMRVPRGFVDQVLW
                     PEFQELNKALVQYLNDVTLRVIREEVHGDASEAAEVPQALPAAASS"
     gene            2761..3603
                     /locus_tag="VGC82_13145"
     CDS             2761..3603
                     /locus_tag="VGC82_13145"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0424260.1"
                     /translation="MPTIDAQVHAYERNHPGRPWAGVLHGPPEVTGNDMVAAMDAVGV
                     DGALLVSPFAMYRYDASYAREVHAAHPGRFGLIKPVDPSDPGVADTIADWAATKGTVG
                     VRIMMNRDVSTDPADPGINRVLATAAKHALPVNILCWGRLEQAGLLAARNPDTMLVID
                     HLGLQQPFEPPPPAEPFADLPKLLSLAQHPNVAVKISGACTLSHERFPYNDLWDPLCR
                     IFDAFGFDRCMWGTDWTRAVELLTYEQGVMAFRVTDRLSDSDRAALMGETLERIYQWS
                     PSKT"
     gene            4021..4452
                     /locus_tag="VGC82_13150"
     CDS             4021..4452
                     /locus_tag="VGC82_13150"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEY0424261.1"
                     /translation="MATRAVDATSSLTGLWLTQDHDGIIRITNCGANLCAEIAGLILD
                     RPNDPTPVDFRGVSQCHLPLITDARPIRPNLWKGHINDPRNGSAYGVELHLDPRGNLA
                     LRGFLGVPLLGHTQTWTRYTGTVPDDCRMHAGSANPTQKRS"
     gene            4816..>5027
                     /locus_tag="VGC82_13155"
     CDS             4816..>5027
                     /locus_tag="VGC82_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0424262.1"
                     /translation="MTTYLVTGGAGFIGSHLCDALIAGGHAVRVLDDLSTGQRTNLPP
                     AATLILGVVAEPEIVAEALDGVDGCF"
BASE COUNT          900 a         1614 c         1544 g          969 t
ORIGIN      
        1 cggcgactgc ctgactggcg atccgggtcg attgctccat ctggcggccg atttcgctga
       61 ccgaggccga caactcctcg gtcgcggcag ccacggtctg cacgttgctg gtcgcctgtt
      121 cggaagccgc cgccacgttg gtgacctggc gcgtggtttc ttccgcggtt tccgccatgc
      181 ggctcgcagt gctgttcaaa tcgcccgcgg acgaggccaa agtttgcaat gcgccagtga
      241 cctgggtttc gaagcgcgcg atcaagccgt caaccatctt cgcgcgctgc tccttcttcg
      301 cctgttcggt gcgctgttcc gcctcgagac gctggcgctc gatcgagcca agcttgaacg
      361 tttcaaccgc gacagcgagc gcgccaattt catccttgcg ctgagctccc ggtatctcga
      421 cgtccagttc cccttcgcta agccggcgag tcaccacggt aagattttcg atcggccgcg
      481 aaatcgccct gccggccata agcgcaagag cgaacgaccc aatgaaaccg gcggccagcg
      541 tcaccgcggc gatcatccat gcgatctgcg aggacgacgt cgcctcatgc tgcgcattga
      601 cgaaggtgct gcgcaactcc ttcgtcagtt cacccaggcg ggcgtcaatg cgcttcgcaa
      661 ccgtttcgag ctgcgccagg gcctgtgcct cggcttgatc gaggcccagg agcagcggcc
      721 cggcataatc caccaaagcc tcggcctgtc gctgtgcttt gacgaacagg cgaacccgat
      781 tgcgctcgag gtcggacgcc ctggtctcac catcggctgc catgatggac gcagcggcct
      841 catggatctg aacattcgcc gccgcgaagt ccttcagcat tttctgcagc ttgtcgtccg
      901 gcgctttgaa gccggcctgc cagcgggcga atgtgctctt gccggcatcc gtcccgccct
      961 tgaagggcgt ctcgaatcga acggcctctc cgaggtcgct gatccatcgc gacagttgga
     1021 cggcctgcag cgacgtgaaa ctggcgaggt ccaggcggcg tccatcgaga tcgaagatgt
     1081 aggcggcacg ccggtcgtgg gcggcgatcg ccgccgtggc ggcggtgctg aggacggcat
     1141 gttccgtcag cgttgcgtca accagttcct tcacccgccc ggttgcggcg atggcgccat
     1201 ggggctcttc tccggcacgg gatgcgacgt cggcatgacc gtcgcggatg ctgagcatga
     1261 ggctgttgaa gcgggacagg tcgttgtcga tcgagccgcg aagctgacca agcccatcga
     1321 gcgaccggct atagcgctgc aggtcggcga tgctgcgttg cagaacctgc gccgcggcaa
     1381 gtcccgcctc gagatccggg acctcctcag ccagcacacc gttagtcacg gtcgtggcat
     1441 gatccagtgc ccaaaggccg acgacaccgc tggtcgaggc gagtaatacg acaacagcga
     1501 agctgccaag cagcttgccg cggatagaac gacgcatctg tgcttctccg tggccaagat
     1561 caattcaggt gctgcagatg ttccagcact tcgaccagtt ccgactttgc ctgctcaagc
     1621 aacgccttgt cgccggtctt cttgtacagt gacaacagca cgaacgaagt cgtggggtgg
     1681 atgatcgtgt cggcatgact gcgggcggcg ctgaattggc tgagtttcgt gatatcgaag
     1741 ccagccgcct tgaatgcttc ggcgccgtcg ccccattgcg cctccagttg ctccggttcc
     1801 attttcttca tcgtatcgac gttggcgatc gtgagttgca tcagcttggc gtattcgggt
     1861 ttgtccaccg caaattcgcg cgcgccctcg acgccgatct tgatgatttc gtcctggcgc
     1921 gccagggttt tgtcgatcga cgggaagttg ccgctgatga cttcgcggat ggtttcctcg
     1981 acttgcgtct tgtaggcaac ggtgtccatc gcctgggcgg cggcgaccgg tgctgcgagc
     2041 accgcgacga gggcgaaagc gcgaaatgac tgcttcatga ctactccgga atggcttacg
     2101 gatcctggga tagctaaaaa cagttaatga aagcataaga aatcaggtga ggatttgtcg
     2161 ctgtctcggc gatcggccca caggcgccgg ccatgccgga tgtttgactg cccggcgcct
     2221 ttctgttgcg gtcaacccgg ccggctcgga aggggaatgc atgcgggagg aagcgcaact
     2281 gcgggaaagg ctgcggaaga tcgaggcgct gttcgccagc gccgggaccg cgggcgaacg
     2341 acttgccgcc gaagcggcac tggaccgggt aggctcgcgt ctggcggcta tgaacgagcg
     2401 tgatccggct gtcgaaatgc agttctcgat gcccgatcaa tggtcccggc ggcttttcct
     2461 ggcgctgtcc cgccgctatg gcctgcagcc tttccgctat cggcggcagc ggttgacgac
     2521 ggtcgtgatg cgggttccgc gcggcttcgt cgatcaggtg ctgtggccgg aattccagga
     2581 attgaacaag gcgttggtgc aatatttgaa cgatgtgacg cttcgcgtta tccgcgaaga
     2641 ggtccatggt gatgccagtg aggcggcgga ggtcccgcag gctctgcccg ccgcggcatc
     2701 atcgtagccc agcagcggaa tttgccggcc gggattacga tcagggaagg gttgcaagtc
     2761 atgccgacca tagatgccca ggttcatgcc tacgagcgca atcatcccgg acggccttgg
     2821 gcaggggttc tgcacggtcc gccggaggtc acgggaaacg acatggtggc tgcgatggac
     2881 gctgtcggcg tggacggcgc gctgctggtt tcgccgttcg cgatgtatcg ctacgacgcc
     2941 agctacgcgc gagaggtcca cgccgcccat cccggacgct ttgggttgat caaaccggtt
     3001 gacccgtccg atcccggagt ggccgatacc atcgccgact gggccgcgac caagggaacg
     3061 gtcggcgtcc ggatcatgat gaatcgagac gtatcgaccg accccgccga ccccggcatc
     3121 aaccgggttc tggccaccgc cgccaagcac gccttgccgg tcaacatcct gtgctgggga
     3181 cgcctggagc aggccggact gctggccgcc cgcaaccccg acacgatgct ggtgatcgac
     3241 cacctcgggc tgcagcagcc gttcgagccg cccccgccgg ccgaaccgtt cgcggatctg
     3301 ccgaagctgc tgtccctcgc ccagcacccc aacgttgcgg tgaaaatcag cggagcctgc
     3361 acgctttcgc acgaacggtt cccgtacaac gacctgtggg acccgctttg ccgcatcttc
     3421 gacgccttcg gcttcgatcg ctgcatgtgg ggcaccgact ggacccgcgc ggtggaactg
     3481 ctgacctacg agcagggggt gatggcgttt cgcgtcaccg accgcctgtc cgacagcgac
     3541 cgggccgctt tgatgggcga gacgcttgag cggatctacc agtggtcgcc gtcaaagacc
     3601 tgatcggcct gctatgggaa aatcaccaat tggcttgtgc tgcaacgaca agcagaccaa
     3661 ccggtggcgg acaatcgccg gtctctggac gactcatccg tttcggcgtc cggtgcgagc
     3721 gaggattcct gtgtcgcaac aaccatctgc agggtcaacc ctggcagcaa ggcggtcgat
     3781 tacgtcaata cctcatcgcg gctgtaaccg acaaaaatgc gacccgcgct gcctgtagcc
     3841 gctccggccg agtcgtcagt cccgacgagg tgtactcaat tagaaccacc cgcgcctgcg
     3901 gacttacttt ttggctgcct ctcggtcatg cccgctgctc ggatcgcgaa cccaaccgat
     3961 gggaccaagc tggccacgat gattagaacg agacgcctcc tggcctgtgt tctgctgtgc
     4021 atggcgacgc gtgcggtcga tgcgacgtct agcctgactg gtctctggct cacgcaagat
     4081 catgacggca tcatcagaat caccaattgc ggcgctaacc tgtgcgcgga aattgctgga
     4141 ctgatcctgg atcgcccgaa cgaccccacc cctgttgatt tccgcggcgt ttcgcaatgc
     4201 cacctgccgc tgatcaccga cgcccgcccg atccgaccca acctctggaa gggccacatc
     4261 aacgatccgc gcaacggcag cgcctatggc gtcgaactcc acctcgaccc gcgggggaac
     4321 ctggccttgc gcggcttcct cggcgttccg ctactgggcc acacccagac ctggacgcgc
     4381 tacaccggga cggtgccgga cgattgccgc atgcatgccg gcagtgcaaa tccgacgcaa
     4441 aaacgctctt gatcacccgc cgcgcagcgg ccgaagtcga tggatccgat catcaacagc
     4501 gaggctggat ctcgttctcc gtggctccgt gttgcgctcc gtgccatccg tattgaagtg
     4561 caatggcaac cgccgcacaa atctccaggt cagccgccca atccaccagc acagaaatcg
     4621 cgaactccag acggaacatt ccgcgctttc atggcgcatg accaacgtgg cacactgtcc
     4681 gcgtaaatat tgcgttcagg atacatctgt tttgaccaaa ttcccgacgg ccgcctttga
     4741 tccatcgccg cctccgaaaa caacgcggag gccatcaggc ggtcgggcca gtcaaggaac
     4801 cgaaggagcc gctgtttgac cacctatctg gtgaccgggg gcgccggctt tatcggctcc
     4861 catctatgcg atgccctgat tgccggaggc catgcggtcc gcgttctgga tgacctgtcc
     4921 accggccagc ggaccaacct cccgcctgcc gcaaccctga tcctgggcgt tgtcgcagag
     4981 cccgaaatcg tcgcggaagc cctggatgga gtcgatggct gctttca
//
LOCUS       DASWQN010000779         4781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2450047, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000779 DASWQN010000000
VERSION     DASWQN010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2450047"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VGC82_13160"
     CDS             complement(<1..243)
                     /locus_tag="VGC82_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424263.1"
                     /translation="MQRGINITAWFRFPASHEPAALARYLSDQALADLRAAGFDFVRL
                     AVDPSVGDTQRAVLITAIRRIQRQGMTVVVSPHPQDW"
     gene            complement(606..878)
                     /locus_tag="VGC82_13165"
     CDS             complement(606..878)
                     /locus_tag="VGC82_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424264.1"
                     /translation="MPLKLASYAGLGVALLSVIYAVQVIVKTLIIGNPVAGYPSLMTV
                     VLFLGGVQLVFLGVIGEYLGRVFNETKQRPLYLVERYVPSDVKISS"
     gene            complement(869..1585)
                     /locus_tag="VGC82_13170"
     CDS             complement(869..1585)
                     /locus_tag="VGC82_13170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0424265.1"
                     /translation="MQEVSSHSPHRSRRPTISVVVPAFNEAIGLKLFHRRLVEALAGV
                     ESWEVIYVNDGSTDATLPVIEALRGAGDHVAVVSLSRNFGKETAITAGLDHAAGDAVV
                     VIDADLQDPPELIPELMACWRAGFDMVYAKRRSRTGDTWLKRGTAKAFYRLMQRMGDL
                     KLPENTGDFRLMSRRVVDAVRQLREQHRFMKGLFAWVGYPTTFVCTTANPGAPAPASG
                     LTGSCGTSHWRGSPALPSCL"
     gene            1648..3138
                     /locus_tag="VGC82_13175"
     CDS             1648..3138
                     /locus_tag="VGC82_13175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0424266.1"
                     /translation="MVNATDLRSKNTFSSFSVPVVAFLVLTGAAWLRSAEYDEQYTLF
                     LTAGVPRPDWPETVFPAGIVAAAQAGHASLDAIARDLRATDVHPPLYFWAVSIWRRIV
                     GPSLFAARMLSVLCGVLTIALVGAVARRCAIRPAIAMLITLGCYGFVYTNAIARGFAP
                     AQTLTLCGIALLLGRRQWLVGLCLGAACCCNYLAAFVAVAAILSVGAWRAVPAMVPFL
                     ALDAWFFVAQHDARPGQFPPFEVWPSLLRLVEYQTASVFGGLPMYLDGAWRIVAGACI
                     GLVAVVVIVGIAGAHFWNAGPAIRVFGAAAIATPIGLLLLGAAFNNTPIELRYLSFGL
                     PFVALLAASAVPRGHGRGCRGSLRRRSWVDWRILVLIIQFGSIIGLLISPRTMQPARI
                     AATEAARLVGNGVVLLPRGNDGVGIVGAFAIEAPAWLPILLIRPGDPVAERVAPYRRV
                     VLALLAQDRDSVASIAGLRAAFALLNWRRVGVGSSVEVYERTGDGQ"
     gene            complement(3263..4006)
                     /locus_tag="VGC82_13180"
     CDS             complement(3263..4006)
                     /locus_tag="VGC82_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY0424267.1"
                     /translation="MRILFANPNTTAAVTDRIAAAARAAASPGTQIVAVTGQTGVPYI
                     ATRADAVIGARVALELLAEHADGCDAAVVAAFADPGVGGARELLSIPVIGMAEAAMLT
                     ACMLGRRFSIVTFATAMGPWYRECVDYNGLSGRLASIRCVSGGFRDINTVQEEKREAL
                     VEACRLAVEQDEADVVILGGAPLAGLAAKVAEDVPVPLVDGVAAAVRQAEVLASLRVR
                     KAVAGTFRRPDAKAVTGVSVGLEGLFYRR"
     gene            complement(4039..>4781)
                     /locus_tag="VGC82_13185"
     CDS             complement(4039..>4781)
                     /locus_tag="VGC82_13185"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0424268.1"
                     /translation="MIALGLVQAALFAPAHETMHQTAFASRRLNAVVGWLAACPSLLN
                     WHFYTAFHLAHHRHTQVADLDPELMAPPPVSLRGYLLRISGVPFWTLRLKVIADCWRG
                     DLSAYPYVSAAAGPKIVRSVRAMTMVMVGGAVASVALFGWATPLLYWIGPQLLGQPPL
                     RAYLLAEHTGCTEDRNGLTNTRTTLTCAPVRLLMWDMPYHAEHHLFPSIPFHRLAEAH
                     GLIRSKLGYLQPGYARWNIGFVRSLMGK"
BASE COUNT          840 a         1571 c         1485 g          885 t
ORIGIN      
        1 ccagtcctgc ggatgcggcg agaccacgac ggtcatccct tgtcgctgaa tgcgtcgtat
       61 agccgtgatc aaaaccgcgc gttgcgtatc gccgacagac ggatcgactg cgagccgcac
      121 gaagtcgaaa ccggcggcac gaaggtcggc caacgcctga tcgcttaggt agcgcgccag
      181 ggcggcgggt tcgtgactgg cgggaaaccg gaaccaagcg gtgatgttga tgccccgctg
      241 caagcggcct gcaaccgcag cgatgggacc gggaagtcgc cacgaggaaa ctgaaccgta
      301 tgacgcactg gtctggcttg gtcgttttat cctgggcgga gacccggcat cggcaccttt
      361 gttggcgtcc gcaatgcaat gcgtggctgg tggggcttcg cccccgatga cgggattggg
      421 ctgcagcgct acgacgggac caggctgcag cgccgtgacc gaggccgcta ctttgccgca
      481 gactattaga tccatggctt gcgacgcgcg cgcgggcgaa agcttggccg gtgggttttc
      541 accggccagt gcactggtcc cgaggcaggc gaccaagccg gccagcaaga cccaccagca
      601 cccgctcacg acgaaatctt tacgtcgctc gggacatacc gctcgaccag atacagcggc
      661 cgctgttttg tctcgttgaa cacccggccc aggtactcgc cgatcacccc cagaaacacc
      721 agttgcacac cgcccaggaa aagcacgacc gtcatcaggc tgggataccc ggcgactgga
      781 ttgccgatga tcagcgtctt cacgattacc tgcacggcgt agatcaccga cagcagcgcc
      841 accccaaggc cggcgtagct cgccagcttc aaaggcatga cggtaaagct ggtgatcccc
      901 tccagtgcga ggttccacag cttccagtaa gaccacttgc tggcgccggc gcaccgggat
      961 tcgcggtcgt acacacaaac gtcgttggat atcccaccca ggcgaacagc cctttcatga
     1021 accggtgctg ctcgcgcaac tgccgcacgg catcgactac gcggcggctc atgaggcgga
     1081 aatcgccggt gttctcgggc agcttcagat cgcccatgcg ctgcatcagg cggtaaaaag
     1141 ccttggccgt tccccgtttc agccaagtgt cgccggttcg gctgcgccgc ttggcgtaaa
     1201 ccatatcgaa gcctgcccgc cagcaggcca tgagttcggg gatcaactcc ggagggtcct
     1261 gcaggtcggc gtcaatcacc accacggcat cgcccgcggc atggtccagt cccgccgtga
     1321 tcgccgtctc cttaccgaaa ttgcgcgaca ggctgacgac agcgacatga tcgccggcac
     1381 cgcgcagtgc ttcgatcacc ggcagcgtgg cgtcggtgct gccgtcgttg acgtagatga
     1441 cttcccagct ttccacgccg gccaaggctt caaccagccg gcggtggaag agcttcagcc
     1501 caatggcctc gttgaaagcc ggaacgacca ccgagatggt tggcctccgg gaccggtgcg
     1561 gcgagtgcga cgacacctcc tgcataagca ctccgttaac ctttggctga tacatccgtg
     1621 tgcaggatgc ggtggaagta ctgagaattg gttaacgcga cggaccttcg ctcgaaaaat
     1681 actttctcgt cgttctcggt gccggtggtc gcgtttctgg tgctgacggg tgctgcatgg
     1741 ttacgcagtg cagaatacga tgagcaatac acgctgttcc tgaccgctgg ggtgccgagg
     1801 ccggattggc cggaaacggt ctttcccgcc ggaatcgttg cggcggcaca ggcgggtcat
     1861 gccagcctgg atgcaattgc ccgcgacctt cgtgccaccg atgtgcatcc gccgctgtat
     1921 ttctgggcag tatcgatctg gcggcggatc gtcgggccca gcctgttcgc ggcgcgcatg
     1981 ttgtcggtcc tgtgcggggt gttgacgata gccctggtcg gcgcggtcgc ccggcgctgc
     2041 gccatccgtc cagcgatcgc gatgctcatc acactcggtt gctatggttt tgtatatacg
     2101 aatgccattg cgcgaggctt tgctccggcg cagacgctga cattgtgcgg gatcgcattg
     2161 ctgctgggcc ggcggcagtg gctggttgga ctgtgcctgg gtgccgcatg ctgctgcaac
     2221 tatctggcgg cattcgttgc cgtggcggcg attctgtcag ttggcgcgtg gcgcgcggtg
     2281 ccggcgatgg tcccattcct ggcgttggat gcctggtttt tcgtcgccca acacgacgct
     2341 cgccccggcc agtttccgcc gttcgaggtc tggccaagcc tgttgcggtt ggtggaatac
     2401 cagacagcat cggtgtttgg tggactgccg atgtatttgg acggagcctg gagaatcgtt
     2461 gccggagcct gcatcggtct ggtcgctgtg gtcgtgatcg tcgggatcgc cggtgcccac
     2521 ttttggaacg caggccccgc tatccgagtg tttggcgccg ccgcgatcgc aaccccaatc
     2581 gggctgttgc tgttgggtgc tgccttcaac aacacgccga tcgaactgcg gtacttgtcg
     2641 tttggactgc cgttcgttgc cttgctggcc gcctcggctg taccccgtgg acatggccgg
     2701 ggttgccgcg gcagcttgcg ccgtcggtcg tgggtggact ggcgaatcct tgtcctcatc
     2761 atccagtttg gcagcatcat cggtctgctg atctcgcctc gcaccatgca gcctgcccgc
     2821 atcgcggcaa ccgaggccgc cagacttgtc ggcaacggtg tcgtcctgct tccccggggg
     2881 aatgacggtg tcggcatcgt cggtgcattt gcgattgaag ctcccgcttg gctgccgatt
     2941 ctcctgatcc ggccgggcga tcctgtggcc gaacgggttg cgccgtatcg ccgcgtcgtg
     3001 ctggcgctgc tggcacagga tcgggatagt gtcgccagta ttgccgggct gcgcgctgcc
     3061 ttcgcgctgc tgaactggcg gcgggtgggg gttgggtcca gcgttgaggt ttatgagcga
     3121 accggcgatg ggcagtagcg tggccgtgct gagagcgctc gaaggaatga aactgaagaa
     3181 aatctttccg ttcaatccgt gcacgaaatc ccagtaatcc gtgatgaaac gatcaccccc
     3241 cgaaccccaa acgcacccca gatcaccgcc ggtaaaacag cccctccaac cccaccgaaa
     3301 ccccagtaac cgccttcgca tcaggccgcc gaaaagtccc cgcaaccgcc ttgcggaccc
     3361 gcagcgacgc cagaacctcc gcctgccgca ccgccgctgc gacgccatcg accaggggca
     3421 ccggcacatc ctcggccacc ttcgcagcca gcccagccaa cggcgctcct cccaggatca
     3481 ccacatccgc ctcatcctgc tccactgcca gccggcaggc ttcgaccagg gcctcccgct
     3541 tttcctcctg cacggtgttg atgtcccgga acccgccgct tacgcatcga atgctggcta
     3601 accgccccga cagcccgttg taatccacgc actcccggta ccacggcccc atagccgtcg
     3661 cgaacgtgac gatggaaaac cgcctcccca acatgcaggc ggttagcatt gccgcctcgg
     3721 ccatcccgat caccgggatc gacagcagtt cccgtgcacc cccaacaccc ggatcggcga
     3781 atgccgccac caccgcggcg tcgcatccgt cggcgtgctc cgccagcaat tccagcgcga
     3841 ccctggcgcc gatgacggcg tctgcccggg ttgcaatgta gggcacaccg gtctgcccgg
     3901 tgaccgccac gatttgggtt cccggactgg cggccgcccg ggctgcggcg gcgatccgat
     3961 ccgtgacggc ggccgtggtg ttcgggttgg cgaacaaaat cctcatgcta ttgccccatc
     4021 aggctattcc ccatcaggct atttccccat tagggaccgg acgaagccga tattccaccg
     4081 cgcgtaaccc ggctggaggt agcccagttt cgagcggatc aacccatgcg cctccgccag
     4141 ccgatggaac gggatcgaag ggaacaggtg gtgctcggca tgatacggca tgtcccacat
     4201 cagcagccgc acgggcgcgc aggtcagtgt ggtgcgtgtg ttggtcaatc cgttgcggtc
     4261 ctcggtgcag ccggtatgtt ccgccagcag atacgcccgc aatggaggct ggccgagcaa
     4321 ttgcggcccg atccagtaca ggagcggcgt cgcccatccg aagagtgcca ccgaggcgac
     4381 cgctccgccc accatcacca tcgtcatcgc gcgcacactg cgaacgatct tcggccccgc
     4441 ggcggccgag acatagggat acgcgctgag atcgccgcgc cagcaatccg cgatgacctt
     4501 cagccgcagc gtccagaacg gcacgcccga tatccgcaac aagtagccgc gcagcgaaac
     4561 cggcggcggc gccatcagct ccgggtccag gtcggccacc tgcgtgtgcc ggtgatgcgc
     4621 caggtgaaac gcggtgtaga aatgccagtt caacagtgat ggacaggctg ccagccagcc
     4681 gacgacggca ttcaatcggc gcgatgcgaa cgcggtctga tgcatggttt catgcgccgg
     4741 agcgaacagc gcggcctgca ccagacccag cgcgatcatc g
//
LOCUS       DASWQN010000780         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_603265, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000780 DASWQN010000000
VERSION     DASWQN010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603265"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VGC82_13190"
     CDS             complement(<1..794)
                     /locus_tag="VGC82_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY0424269.1"
                     /translation="MTRSHNIAVLPGDGIGKETVPESLKVLDAAARRFGFNLNLTHYD
                     WSCETYKNTGMMMPEDGLDQLRKSDSILLGAVGWPGVPDHVSLWGLLIPIRRGFDQYA
                     NVRPCKLLPGVRTPLAGRTEADIDFYVVRENTEGEYSSVGGRMFPGTDREFVSQQSVF
                     TRQGVDRILKYAFELAMTRPKKHVTSATKSNGIIHTMPYWDERFAEMAGRYPEIRTDQ
                     YHIDILCAHFVQHPDWFDVVVGSNLFGDILSDLGPACTGTIGIAPAA"
     gene            887..>2256
                     /locus_tag="VGC82_13195"
     CDS             887..>2256
                     /locus_tag="VGC82_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HEY0424270.1"
                     /translation="MAFAGFQDYLWGMHISTSPAFKENAHLALGNPGLQKALARSGPS
                     FIARRAAAVAGLPEFEKLRDTARDIKNHALANLDFYLEAYEAKVLESGGKVHWCSDAD
                     EARDAVLAICQAAGARTVTKGKSMIGEEIAINDHLERHGITPVETDLGEYIIQLRHEL
                     PSHIIAPAFHLNMEDWEAAFRKAHTDLPADRTFNERRDILVEARTKLRSSFLAADVGI
                     TGANFLIAETGSSVIVTNEGNGDLTQTLPRVHIVLASIEKCVPTLEDATSLLRVLARS
                     ATGQDFSVYTTFSTGPRRAGDLDGPEEYHVVLIDNGRSAMLGTEFQEMLRCIRCAACM
                     NHCPVYGAVGGHAYGWVYPGPMGAVLTPSLIGVEKAGHLPNASTFCGKCESVCPVRIP
                     LPKLMRHWREREFERHLTPATYRSNLALWAWFAKRPSAYRTVTRVAAAALGSIGRRRG
                     RFGSLP"
BASE COUNT          414 a          697 c          746 g          399 t
ORIGIN      
        1 gcggccggcg cgatgccgat ggtgccggtg caggccggcc ccagatcgga caggatgtcg
       61 ccgaacaggt tggagccgac gaccacgtcg aaccaatccg gatgctgcac gaaatgggcg
      121 cacaggatgt cgatgtggta ctggtcggtg cgaatctccg gatagcgccc ggccatctcg
      181 gcaaagcgct cgtcccagta tggcatcgtg tgaatgatgc cgttcgactt cgtggccgac
      241 gtgacgtgct tcttgggccg cgtcatggca agctcgaagg cgtatttcag gatgcgatcc
      301 acgccttgac gggtgaagac gctttgctgg ctgacgaact ccctgtccgt gccagggaac
      361 atccggccgc cgaccgacga gtattcgccc tcggtatttt cccgcacgac atagaaatcg
      421 atatcggctt cggtgcggcc ggccagcggc gtgcggaccc ccggcaaaag tttgcacggg
      481 cggacattgg catactggtc gaagccgcgg cggatcggga tcagcaggcc ccagagtgag
      541 acatggtccg gcacgccggg ccagccgacc gcgcccaaca ggatggaatc cgatttgcgg
      601 agctggtcca ggccgtcttc cggcatcatc atgccggtat tcttataggt ttcgcacgac
      661 cagtcgtaat gggtcagatt caggttgaat ccgaagcggc gggcggcggc atccagcacc
      721 ttcagcgact caggcacggt ttccttgccg atcccgtcgc ccggtagaac cgcgatgtta
      781 tggctgcgcg tcatggctgt ttcctagaaa agcggtagcc gcaggaataa cggagccggc
      841 acgacacgga aaccccgctg gcgccaagcc gacccgacca cgacccatgg ctttcgccgg
      901 ttttcaggat tatctctggg gaatgcatat ctcgacctcg ccggcattca aggaaaacgc
      961 ccacctggct ttgggcaatc cggggctgca gaaggcgctg gcccgcagtg gccccagctt
     1021 catcgcccgg cgggcggcgg ccgtggcggg gttgccggag ttcgagaagc tgcgggacac
     1081 cgccagggac atcaagaacc acgccttggc gaacctggac ttctacctgg aagcctatga
     1141 ggcgaaggtg ctcgaatccg gcggcaaggt gcactggtgc tccgatgccg acgaagccag
     1201 ggacgccgtc ttggcgatct gccaggcggc aggcgcgcgg accgtcacca aaggaaaatc
     1261 gatgatcggc gaggagatcg ccatcaacga ccatctggaa cgccatggga ttacgccggt
     1321 cgagaccgat ctgggcgagt acatcattca gctccggcac gagctgccga gccacatcat
     1381 cgctccggca tttcacttga acatggagga ttgggaggca gctttccgga aagcccatac
     1441 cgacctgccg gccgaccgca ccttcaacga gcgtcgcgat atcctcgtgg aggcccgaac
     1501 caaattgcgg tccagttttc tcgcggcgga cgtggggatt accggggcaa actttctgat
     1561 cgccgagacc ggcagcagcg tgatcgtgac gaacgaaggc aacggcgacc tgactcagac
     1621 tttgccccgg gttcatatcg tcctggccag catcgagaaa tgcgtgccaa ccctggagga
     1681 cgcgacttcc ctgctgcggg tgctggcgcg gtcagccacc gggcaggact tttccgtcta
     1741 tacgacgttc tccactgggc cgcggcgggc cggggacctg gacgggccgg aggaatacca
     1801 cgtcgtcctg atcgacaacg ggcggtcggc gatgctcggg accgagttcc aggagatgct
     1861 gcggtgcatc cgttgcgccg cttgcatgaa ccactgcccg gtttatggcg cggtcggcgg
     1921 tcatgcctat ggctgggttt atcccggtcc gatgggcgcg gtgctgaccc caagcctgat
     1981 cggggtggaa aaagccgggc atctgccgaa tgcctcgacg ttctgtggca agtgcgaaag
     2041 tgtctgcccg gtgcggattc ccctgccaaa gctgatgcgc cactggcgcg agcgggagtt
     2101 cgagcgccac ctgacgccgg cgacctatcg cagcaatctc gccctctggg cgtggttcgc
     2161 gaagcggccc tcggcgtacc gcaccgtgac gcgggtggcc gccgcggcgc tcgggtcgat
     2221 cggtcggagg cggggccgtt tcggctccct gccgat
//
LOCUS       DASWQN010000781         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1526067, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000781 DASWQN010000000
VERSION     DASWQN010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526067"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..983)
                     /locus_tag="VGC82_13200"
     CDS             complement(123..983)
                     /locus_tag="VGC82_13200"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424271.1"
                     /translation="MNLHQRGFALLIVLWTVGFLALLGTQIVAAGRSDTQLADNLKQE
                     GVLQAAAEGAVANVMFRILAAKDPGFAPDGMVREVRIGRTLVLVQVEDESDRVNLNTA
                     SGVLLRALIVEVGLPPAQATRLAASILDWRTSGANPRSGGAKAADYRAAGRAYGPPGT
                     PFQSVGELADVLGMTPELLARLEPHLTVLTDGDPDLSTRDPVVARALTDAAGVADDTA
                     AAQQTADQLLRVSVSAVGRNGARYSLVVVASADFQNRSPRVNMLLRERGGGLTMGSAT
                     SLAGRSDAAR"
     gene            complement(980..>1503)
                     /locus_tag="VGC82_13205"
     CDS             complement(980..>1503)
                     /locus_tag="VGC82_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424272.1"
                     /translation="GFLLAGLSQTVRFGLTAWRQHAHLSDDKTDLEAVERSLRAVVEN
                     LSPGDDQARPPIEGSADALTGVTRLRVPNGGSTPIRVEAGLAVSGTRLVLRWRPHHHG
                     PPLSPPLPPAETELVGGVARLRIEYWRPPGIWVTRWREPDLPLLIRFQLSFAGPYAPR
                     WPDIVVAPLLSRP"
BASE COUNT          272 a          522 c          466 g          243 t
ORIGIN      
        1 tatatcatca attataatta tcttgatatg attgcgttgg ccagaataaa acgatctctt
       61 cctcgtccgg aaccaagcag gagattctgt taacctgatt gcaatacgag accgcttgcc
      121 cgtcacctcg cggcatcgga cctgcccgcc aaggaagtcg cggaacccat cgtcagaccg
      181 ccgccgcgtt cccgcaacag catgttcacg cgcggggagc ggttctggaa gtcggccgat
      241 gccacgacga ccagcgaata ccgcgctccg tttcgcccca cggcggacac actgactcgc
      301 agcaattggt cggcggtctg ctgtgccgca gcggtatcgt cggctacgcc cgcggcgtcc
      361 gtcaacgccc gcgccaccac cggatcgcgc gttgacaggt cgggatcgcc atccgtcagc
      421 accgtcagat gcggctccag gcgcgccaac agctccggcg tcatgcctag cacgtccgcc
      481 agttcgccaa cgctttggaa tggtgttccc ggcggcccgt aggctcgccc cgccgcccga
      541 tagtcagccg ccttggctcc gccggaccga ggattggcac ccgacgtccg ccagtccagg
      601 atcgaggccg cgaggcgggt ggcctgtgcc gggggcaagc caacctcgac gatcaaagca
      661 cgcagcaaaa cgcccgatgc ggtgttgaga ttgacccggt cgctttcatc ctcgacttgc
      721 accagaacca gtgtccggcc gatccggacc tctcggacca tgccgtcggg cgcgaacccg
      781 gggtccttcg cggccagaat ccggaacatg acattggcaa cggcaccttc cgccgctgcc
      841 tgcagcacac cctcttgttt cagattgtcg gccagttggg tgtcggaccg cccagcggcg
      901 acgatttgcg tccccagcag cgcgagaaat cccaccgtcc aaagcacaat cagcaaggcg
      961 aagccgcgtt gatggaggtt catggccgcg acagcagcgg cgccacgacg atgtccggcc
     1021 accgtggcgc gtatggacca gcgaagctca gctgaaaccg gatcaacagc ggaaggtccg
     1081 gctcccgcca gcgtgttacc cagataccgg gcggccgcca gtattcgatc cgcaggcggg
     1141 ccacgccgcc gaccagttcc gtttccgccg gcggcagtgg tggtgacagc ggcggtccgt
     1201 ggtgatgcgg acgccagcgc aacaccagcc gcgtgccgga taccgccagt ccggcttcca
     1261 cccggatcgg cgtcgatcct ccgttcggga cgcgcaggcg ggtcacgccg gtcagggcat
     1321 cagccgaccc ctcgatcggc ggccgagcct gatcgtctcc gggggaaagg ttttcgacga
     1381 cggcacgcag gctgcgctcg accgcttcca ggtcggtctt gtcgtcggac agatgggcgt
     1441 gctgccgcca agcggtcagg ccgaaccgca ccgtctggct caggccggcc agcaggaagc
     1501 caa
//
LOCUS       DASWQN010000782         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_603673, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000782 DASWQN010000000
VERSION     DASWQN010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603673"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /gene="pgk"
                     /locus_tag="VGC82_13210"
     CDS             <1..421
                     /gene="pgk"
                     /locus_tag="VGC82_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY0424273.1"
                     /translation="AIILPVDAVVAFHFAAHAPSHAYGLDAIPPEGMILDVGPQSVAR
                     IHAAIDDAATLVWNGPLGAFELPPFDRGTMVAARHAAERTRAKKLISVAGGGDTVAAL
                     NQAGVAGDFTYVSTAGGAFLEWMEGKPLPGVEVLRIH"
     gene            470..1537
                     /gene="fba"
                     /locus_tag="VGC82_13215"
     CDS             470..1537
                     /gene="fba"
                     /locus_tag="VGC82_13215"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644240.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY0424274.1"
                     /translation="MARITLRQLLDHAAEHDYGVPAFNINNMEQALAIMEAASSLDAP
                     VIIQASRGARSYANDVMLKHMMDAVTEIYPQIPVCVHLDHGNEPATCMTAIQAGFTSV
                     MMDGSLKTDGKTPGDWDYNVGVTKKVTDMAHLGGISVEGELGVLGSLETGMGDKEDGH
                     GAEGKLSHDQLLTNPDEAVKFVKETKVDALAIAMGTSHGAYKFTRKPDGDILAMNVIE
                     EIHRKLPNMHLVMHGSSSVPQDLQDIINANGGKMKPTWGVPVSEIQRGIKNGVRKINI
                     DTDNRMAMTGQIRKVLNDNPEEFDPRKYLKPAMEAMAKLCKQRLQEFNTAGQASKIKK
                     VLTTAEMAKRYVKGELDPKVA"
     gene            1671..>2505
                     /locus_tag="VGC82_13220"
     CDS             1671..>2505
                     /locus_tag="VGC82_13220"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY0424275.1"
                     /translation="MNLAELNKVALALVAPGKGILAADESSGTIKKRFDGINVESTEE
                     SRRDYREMLFRSSEAMTKSISGVILYDETIWQNAKDGTPLVKIIEQSGAIPGIKVDEG
                     TQAVPNCPGELVTVGLDKLAERLKKYYDRGARFAKWRAVINIDKDKIPSMTAIHVNAH
                     ALGRYAALCQAAQIVPIVEPEVLMDGDHDIDRCYEESQRVLNETFQELRIQRVALEGM
                     VLKPNMVVPGKKSTKQASVEEVAEKTIRLLKNCVPAAVPGIAFLSGGQSDEDATAHLD
                     AM"
BASE COUNT          572 a          752 c          749 g          432 t
ORIGIN      
        1 cgccatcatc cttcccgtgg acgccgtggt cgcatttcac ttcgcggccc atgcgccttc
       61 ccatgcctac ggtctcgatg cgatcccccc tgagggcatg atcctcgacg tcggcccgca
      121 gtcagtggcg cggattcatg ccgcgatcga cgacgccgcg acgctggtct ggaacggccc
      181 gctcggggcg ttcgagctgc cgccgttcga ccgcggcacc atggtagcgg caaggcacgc
      241 ggccgagcgg accagggcga agaagctgat ctcggtcgct ggcggcggcg acaccgtggc
      301 ggcgctgaat caggccggcg tggccggcga tttcacctat gtttcgaccg cgggcggtgc
      361 gtttctcgaa tggatggaag ggaaaccctt gcccggtgtc gaggttttga gaatacacta
      421 gatcaaggcc ggatcgggca ttgaggcctc aacgcggaga acaggacaca tggctcgcat
      481 cacgttgcgt caattgctcg accacgcggc ggaacacgat tacggcgtgc cggccttcaa
      541 catcaacaac atggagcagg cgctcgccat catggaagcc gcctccagcc ttgatgcgcc
      601 tgtcatcatt caggcctcgc gcggcgcgcg ctcctacgcc aacgacgtca tgctgaagca
      661 catgatggat gcggtcaccg aaatctatcc gcaaataccg gtctgcgtgc acctcgatca
      721 cggcaacgaa cctgcgacct gcatgaccgc gatccaggcc ggcttcacct cggtcatgat
      781 ggacggctcg ctaaagaccg acggcaagac acccggcgac tgggattata acgtcggggt
      841 caccaagaaa gtcaccgata tggcccatct cggcggcatt tcggtagaag gcgaacttgg
      901 ggtgctgggt tcgctcgaga ccggcatggg cgacaaggag gacggtcacg gtgccgaggg
      961 caagctctcc cacgaccagt tgctcaccaa tcccgacgag gcggtcaagt tcgtcaagga
     1021 gaccaaggtg gacgcactgg ctattgccat gggcacgtcg cacggcgcct acaaattcac
     1081 ccgcaagccg gatggcgaca ttttggccat gaatgtgatc gaggaaattc accgcaagct
     1141 gccgaacatg catctggtga tgcatggttc gtcgtcggtg ccgcaggacc tgcaggacat
     1201 catcaacgcc aatggcggca agatgaagcc gacctggggc gtgcccgtgt ccgagatcca
     1261 gcgcggcatc aaaaacggcg ttcgcaagat caacatcgac accgacaacc gcatggcgat
     1321 gaccggacag ataagaaagg tgctgaacga caatccggaa gaattcgacc cgcgcaaata
     1381 tctcaagcca gcgatggaag cgatggccaa gctatgcaaa cagcggctgc aggaattcaa
     1441 caccgcaggt caggccagca agatcaagaa ggtgctcacc accgccgaga tggcgaagcg
     1501 ttacgtcaag ggcgagctcg atccgaaagt cgcgtgacag gccactcccg agcgaaccgc
     1561 cagccacccc gcgccgcgcg cgttttctta ggaattgcct gattcttggg aattgcctga
     1621 gaacggtcta cattgacttg caaatatcca gtgctcagga ggaggcagcg atgaatctcg
     1681 ccgaactcaa caaggtcgct ctggccctgg tcgcgccggg caagggcata ctcgccgcgg
     1741 acgaatcctc aggcacgatc aagaagcgct tcgacggcat caacgtcgaa tcgaccgagg
     1801 aatcgcgccg cgactatcgc gaaatgctgt tccgctccag cgaagcgatg accaagtcca
     1861 tttccggtgt catcctctat gatgagacca tctggcaaaa tgccaaggat ggcacgccgc
     1921 tggtgaagat catcgaacaa tcaggagcga tccccggcat caaggtcgat gagggcacgc
     1981 aagccgtgcc gaactgcccg ggtgaattgg tcaccgtcgg gttggacaag ctcgccgagc
     2041 gtctaaagaa atattacgat cggggcgcgc ggttcgccaa atggcgcgcg gtgatcaata
     2101 tcgacaaaga taagatcccg agcatgaccg caatacacgt caatgcccac gcgctcgggc
     2161 gttacgctgc attatgccag gccgcgcaga ttgttccgat cgtcgaaccg gaagtcctga
     2221 tggatggcga tcatgacatc gaccgctgtt atgaagaaag ccagcgcgtt ctcaacgaga
     2281 cctttcagga attgcggatt cagcgcgttg cattggaagg catggtcctg aaacccaaca
     2341 tggtagtccc cggcaagaaa agtaccaagc aagcttccgt tgaagaggtc gcggagaaaa
     2401 ccattcggct attgaagaac tgcgttcccg cggcggtgcc cggcattgcc ttcctctccg
     2461 gcgggcagtc tgacgaggac gccaccgcgc atctcgacgc catga
//
LOCUS       DASWQN010000783         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2318539, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000783 DASWQN010000000
VERSION     DASWQN010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2318539"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            459..1691
                     /locus_tag="VGC82_13225"
     CDS             459..1691
                     /locus_tag="VGC82_13225"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0424276.1"
                     /translation="MTQATFAALGLAEPLLRALEARKFEIPTPIQADTIPALLANRDL
                     LGVAQTGSGKTAAFTLPMLQHLAAQQIRPAPFVARALILAPTRELALQIDETVRALSI
                     NLRLRTVVIIGGASRFKQVEAMRRGADIVIGTPGRVCDLMQGRELQLGAVSRFVLDEA
                     DRMLDLGFIKDIRRIVAALPSQRQSCLFSATMPAEVASLANSLLRDPMRVEIARKEET
                     TPKIEQFVHHLPQSGKQSLLLSMLQDDALSRVIVFTRTKHGANKVAAVLENAGIQVNA
                     IHGNKSQPQREKALRDFRTGRARVLVATDIAARGIDVTGISHVINFDLPVEADSYVHR
                     IGRTARAGAAGIAISFCDPTERGVLRQIERQMNLTIGVVGDAPAPMTHQPNRPSAPGQ
                     AQRRRYNDRGNRGMRSAA"
     gene            1798..2262
                     /locus_tag="VGC82_13230"
     CDS             1798..2262
                     /locus_tag="VGC82_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003623258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEY0424277.1"
                     /translation="MTTLYIDGDACPVRDEAYRVADRLGLPVFVVANGSRPIRPTGRP
                     NVTMITVKAGADVADDWIAERVTKADICITSDIPLASRCLTKGGRALAPNGKIWTRDN
                     IGNALAGREIARHLREIGLNTGGPAPLTKQDRSHFLSALDNLVRAALRERVE"
     gene            2259..>2711
                     /locus_tag="VGC82_13235"
     CDS             2259..>2711
                     /locus_tag="VGC82_13235"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEY0424278.1"
                     /translation="MTDKVLGVLGGMGPLASAQFMLRLTLLTPAGRDQDHIPAVLWSD
                     PRVPDRSAAQSGTGPDPLPWLMRGIDGLTRAGCGAIAIPCNTAHGWYDAMAKGAGVPI
                     LHIVDATAADLRRIGILRGTIGVLGTAATLAMGLFQERLSVAGWDTIVP"
BASE COUNT          501 a          886 c          841 g          483 t
ORIGIN      
        1 gcatgccggt ggctgaatca catcgatgac ctgatattgc agtcgatgcc gtccctgacc
       61 cacgttgcga aactggacat gtcgatcgca cctcatgccc tgaacgcgat gcggtgctgg
      121 aaatgaatga ccgatcgttc ggcgattttt cgaacctgca ggtcgagaat tggatgatcg
      181 acctcgaacg gctcgtcgcg tgaccacggc cgtcgcgtaa caacgctccg tcgatcaggg
      241 cacaacgcag ttgcacaaaa cccttgcaat tacggaaccc atggccgata taccggatgt
      301 ctttccgtga ttgcgtgatt aacgggcggt tatcctcgtc cgaagtccgg aatttcccat
      361 ctgaacaccg ttcgtcccca tttaatcgcg cggggcgatg aactcacagg cgctcgccgt
      421 ccatatgaca gtggcgtgcg ctggagcata cccaggtttt gacccaagct actttcgcgg
      481 ctctcggcct cgccgaaccg ctgctgcgcg cacttgaagc ccgcaaattc gagattccta
      541 ctccgattca ggccgacacc atcccagcgc tactggccaa cagggatctg ctcggcgtcg
      601 cccaaaccgg cagcggcaag accgcggcct ttacactccc gatgctgcag cacctcgcgg
      661 cgcagcaaat ccgtcccgcg ccgttcgtgg cccgggcgct gatcctcgct ccgacgcgtg
      721 aactggcgct tcagatcgac gagaccgtcc gcgcgttgtc gatcaacctg cgtctgcgta
      781 ccgtggtcat cattgggggt gcgagccgct tcaaacaggt cgaggccatg cgccgcggcg
      841 ccgatatcgt catcggtacg ccgggccgcg tgtgcgacct gatgcaggga cgcgaactgc
      901 aactcggcgc cgtgtcccgc ttcgtcctgg atgaggctga ccggatgctc gaccttggct
      961 ttatcaagga catccgccgg attgtagcgg ccctgccgtc gcaacgtcag tcttgccttt
     1021 tctcggccac aatgccggca gaggtcgcga gcctggcgaa tagcctgctg cgtgacccga
     1081 tgcgggtgga gatcgcccgc aaggaagaga ccacaccgaa gatcgagcag ttcgtccatc
     1141 atctgccaca gagcggcaag cagtcgctgc tgctgtccat gctgcaggac gatgcgctca
     1201 gccgggtgat cgtattcact cgcaccaaac atggcgccaa caaggtcgcc gcggtgctgg
     1261 agaacgcggg aatccaggtg aacgcgattc acggcaacaa aagccagccg cagcgtgaga
     1321 aagccttgcg ggacttccgc accggccggg cacgcgttct ggttgcgacc gacatcgcgg
     1381 cccgggggat cgacgtgacg gggatctccc acgtcatcaa cttcgatctt ccggtcgaag
     1441 cggatagcta tgtccatcgc attggccgca ccgcgcgggc cggagccgcg ggcattgcca
     1501 tctcgttctg cgacccgacg gagcgtggcg tgctgcgcca gatcgagcgt cagatgaacc
     1561 tgacgatcgg cgtggtgggt gatgcgccgg ctccgatgac gcaccagccg aaccgcccca
     1621 gtgcgccagg gcaggcgcag cggcgccggt acaacgaccg cggcaatcgg ggcatgcgct
     1681 ccgccgcgta actcggaccc cgtccagaca gcgtgtctga acgggtaaag attccaccta
     1741 aaacatgtgc taaagctttt cgctgagcca acgcgaacgg aaaaagtttt agccgggatg
     1801 accaccctct atatcgacgg cgatgcctgt ccggtccgcg acgaagccta tcgcgtcgcg
     1861 gaccggcttg gcttgccggt tttcgtcgtt gccaacggct cgcgcccgat acgcccgacc
     1921 ggccggccga acgtcaccat gatcacggtt aaagctggtg cggacgtcgc cgatgattgg
     1981 atcgccgaac gtgtcaccaa ggcggatatc tgcatcacca gcgacattcc gctggcctcc
     2041 cgctgcctga cgaaaggtgg ccgagccttg gcgccgaacg ggaagatatg gacccgggac
     2101 aacatcggca acgcactcgc cggccgcgag atcgcgcgcc atctgcggga gatcgggctg
     2161 aacaccggtg gtccggcgcc tctcacgaag caggaccgct cgcactttct cagcgcattg
     2221 gataacctgg tgcgggcggc actacgagag cgggtcgaat gacggacaag gtccttggcg
     2281 tcctgggcgg tatggggcca ctggcgagcg cgcagttcat gctgcgccta acgctgctga
     2341 ctccggccgg ccgcgatcag gatcatattc ccgccgtcct ttggtcggac ccgcgcgtcc
     2401 cggaccgaag cgccgcccaa tccggaaccg gtccggatcc gctgccctgg ctgatgcgcg
     2461 gcatcgatgg cctgacccgc gcaggttgcg gcgcgatcgc catcccctgc aacaccgcgc
     2521 atggctggta cgatgcgatg gccaagggcg ccggcgtgcc gatcctgcac atcgtcgatg
     2581 caaccgcggc cgatctgcgg cgcatcggga tcctgcgcgg caccattggc gttctgggga
     2641 ctgccgcgac cctagccatg gggctatttc aggagcggct tagcgtcgcg gggtgggaca
     2701 ccatcgtgcc g
//
LOCUS       DASWQN010000784         3904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_76205, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000784 DASWQN010000000
VERSION     DASWQN010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76205"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            897..>3904
                     /locus_tag="VGC82_13240"
     CDS             897..>3904
                     /locus_tag="VGC82_13240"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEY0424279.1"
                     /translation="MTASASSQTGLGLPQSLFIPNVTPDNGLSAPFNSFFTFFGQFFD
                     HGLDKITTDSLGGSSFVFIPLAKDDPLYNPATPGNNFMVETRAQNQAGPDGILGTADD
                     VHQFSNGDTPFVDQSQTYASDPSHQAFLRAYMIGSDGKLHSTGALLGQTGIDGHDSMA
                     TWGELKANALQFLGIKLDDTNDVGNVPLLAVDAYGNLILGPHGLAQLVENVPGSVPAR
                     QILREGNLAAPISTDANTLRTGNAFINDMAQGASPVDPQFGQPVPYNHALLDAHYVAG
                     DGRVNENIGLTAIQNIFHSEHDRILASIQKLVQDNLNAGDTGYATNWVLPGVILTGPV
                     LDAAGRVITPATQIAANQWNGERLFQAAKFTTETEYQHIVFDEFARKVAPAIHVAGGV
                     NLHINPAITSEFANVVYRFGHSMLDENINLYVLGADGKPVMGANGQPQMTTEGLIQAF
                     TNPLQFAATPNATADIVLGTVNQVGNEIDEFVTGTLQNNLLGQPLDLASLNIARGRDT
                     GVAPLNEVRNQLFSQTGESQLKPYVSWRDFGGQLKHIESLVNFVAAYGTHASITSATT
                     NAAKLLAAATLVAAGTLGSPTFNIDAYNFMNSVGAYASNKLDTRALHDSNGIPAVWGT
                     GSVTGVDNIDMWIGGLAERQTLFGGVLGSTFEYIFRTQMENLQDADRLYYLPRIEGMD
                     YEESLQDSSLAQLVRANTNIKHLSGDIFQTPEYNVEASTYYLKDANGNVILDANNNPT
                     FKKNADGTFDTTNWLRNATTGTLLVNVLADGTVKFIDDENFLGNTIVLGGTEGNDKLT
                     AGAGGGNTIWGDGGNDVLDGGLGNVPQFLFGGDGNDTLYGGAAADTLHGDVGDDTIYG
                     GDGIDNIFGGDGNDWIEGGRGDDIIMGGLGNDVIMGNEGHDELTGGEGDDWLDGRGGQ
                     GQLMFGDSGAPSGGQPLYSGNDVMIGGANGGDIFKGFSGDDIMVGNGSFTKFIGGLGF
                     DWGSYETAIHGVSEDMNRKEFITPSGLAEDS"
BASE COUNT          861 a         1259 c         1088 g          696 t
ORIGIN      
        1 tcggccagag cggcacgtag cccagcttgt cgatccagtt cagatctcga tcgaatcgat
       61 agatgcaccc cgtcatgcag ctttggccaa cgtgcgcagt cgctcgggga cccgctcggg
      121 accaagatgc cccggagcgg ccgacgccgt cggattcggg tcggagttca gaatcattac
      181 cgataacttc atagtgattt cagaataaga aatatatcga tatagttcgg attacggaat
      241 ttctaatacc aactcggcga actgacacgt agatcatgct cacggtcatt gcgttgtagt
      301 actcgtcgaa acatcaatgt aatggagata cgcacatgac gatcaaaatc actcgtctcg
      361 acctcgacta tatcctgaca cagatcgaga tggctgaagg gggccagccg ccagtcaatc
      421 cgctgctgtc attcggcctg cgcgaagtcg ccggcacgaa caacaacctt gtgcccggtc
      481 agagcaattt cggcgcctct aatcagctgt ttcctaccct gagcacccca atcctgcaga
      541 atgcgcagaa cggcacttcc tatgcccaga ccagcggact ggtccaggac gcgtcgccgc
      601 gcaccatcag cctgctgatc gccagccagg acgccaacag ccagctgacg cccagccagg
      661 aaatcgtagc ggctggcgcg gacaagattc tcggcaccgc ggacgatgtc ttcgtgaacg
      721 gcaatgccgc cgccttcgcc gcccagcaac atgccctcgg cctgcttggc attggctatc
      781 agaacctgac gctgcccggc gcggacggca tttacggcac cgcggacgat accggcacca
      841 ccgtcacctg ggcggacggc accgtccaca cgacattcgg caacctcgcg acaccgatga
      901 ccgcctccgc cagcagccag accggccttg gcctgcccca gagccttttc atccccaacg
      961 tgacacctga caacggcctt tctgccccat tcaacagttt cttcacgttc tttggccagt
     1021 ttttcgacca cggcctcgac aagatcacca ccgattcgct tggcggctcc agcttcgtct
     1081 tcataccgct ggcgaaggat gaccccctgt acaatccggc gacacccggc aacaacttca
     1141 tggtggaaac ccgcgcgcag aaccaggccg gcccggacgg gatcctcggc accgccgacg
     1201 acgtccacca gttcagcaac ggggacaccc ccttcgtcga ccagagccag acctatgcct
     1261 ccgacccatc gcaccaggca ttcctgcgcg cttacatgat cggctcggac ggtaagctgc
     1321 actccaccgg tgccttgctg ggccaaaccg ggattgacgg ccatgacagc atggcgacct
     1381 ggggcgagct caaggccaac gcgttgcagt tcctcggcat taaacttgat gacaccaacg
     1441 acgttgggaa cgtgccgctg ctggctgtgg acgcctacgg caacctgatc ctcggcccgc
     1501 acggactggc gcagctggtc gagaacgtcc ccggttcagt cccggcgaga cagatcctgc
     1561 gtgagggcaa ccttgcggcc ccgatttcca ccgacgccaa cacgctccgc accggcaatg
     1621 ccttcatcaa cgacatggcg cagggcgcct ccccggttga tccgcagttt ggccaacccg
     1681 tgccctataa ccacgctctg ttggatgcgc actacgtcgc gggcgatggc cgggtgaacg
     1741 agaatatcgg cctcaccgcg atccagaaca ttttccattc ggagcatgat cggatactcg
     1801 cgtcgatcca gaagctggtg caggacaacc tcaacgccgg agataccggc tacgcgacca
     1861 actgggtgct tccgggcgtc attctgaccg gtccggtcct tgacgccgct ggtcgggtca
     1921 ttacacctgc gacgcagatc gcagctaacc agtggaatgg cgaacgcctg tttcaggccg
     1981 ccaagttcac taccgaaacc gaataccagc acatcgtctt tgatgagttc gcccgcaaag
     2041 tcgccccggc aattcatgta gccggcggtg tgaacctgca catcaatccg gcaattacat
     2101 cagagttcgc caacgtcgtc tatcgcttcg ggcattcgat gctggacgag aacatcaatc
     2161 tctacgtgct cggtgcggac ggtaagcctg tgatgggcgc caacgggcaa ccacagatga
     2221 caaccgaagg cctgatccag gcgttcacca acccgctgca atttgccgct acacccaacg
     2281 caaccgccga catcgtgttg ggcaccgtca accaggtcgg caacgagatc gacgagtttg
     2341 tcaccggcac cctgcaaaac aaccttttgg gtcagccgct ggaccttgcc tcgctcaaca
     2401 tcgcccgcgg ccgtgacacc ggcgttgcgc cgctgaacga ggtgcgtaac caactcttta
     2461 gccagaccgg cgaaagccag cttaagccat acgttagctg gcgggatttt ggcggccagt
     2521 tgaaacacat cgaatctttg gtcaacttcg ttgctgccta tggcacgcac gccagcatta
     2581 ccagcgccac gacgaacgcc gccaagctgt tggcagcggc cactctggtt gccgcgggaa
     2641 cactcggcag ccccacgttc aacatcgacg cctacaattt catgaacagc gttggcgcct
     2701 atgcgagcaa caagctcgac acccgagccc tgcacgactc gaatggcatc ccagcggtct
     2761 ggggcaccgg ttcagttacc ggtgtggaca acatcgatat gtggattggc ggcctcgccg
     2821 agaggcagac cctgttcggt ggtgtgctcg gctctacctt cgaatacatc ttccgcaccc
     2881 agatggaaaa cctgcaggac gccgaccggc tgtactacct gccgcgcatc gaaggcatgg
     2941 actacgaaga atcgctgcag gacagttcgc ttgcgcagct ggttcgagcg aacaccaaca
     3001 tcaagcattt gtccggcgac atattccaaa ccccggaata caacgtcgag gcgagtacct
     3061 actacctcaa ggatgcgaat ggaaacgtaa tcctggatgc caacaacaac ccaaccttca
     3121 aaaagaacgc cgatggcacg ttcgacacga ccaactggct aaggaacgcg acgaccggca
     3181 cactcttggt caacgtgctg gccgacggga cggtcaagtt catcgacgat gaaaacttcc
     3241 tcggcaacac catcgttttg ggcggcaccg aaggtaacga caagctgacg gcgggcgcgg
     3301 gaggcggcaa cacgatctgg ggcgacggcg gcaacgacgt gcttgacggc ggtctcggca
     3361 acgtccctca attcctgttc ggaggcgacg gaaacgacac gttgtatggc ggcgcggccg
     3421 ccgacacgct tcatggcgac gtcggcgacg acacgatcta cggcggcgac ggcatcgaca
     3481 acatcttcgg tggcgatggc aacgattgga tcgaaggcgg ccgcggcgac gacatcatca
     3541 tgggcggcct cggcaacgac gtcatcatgg gcaacgaggg ccatgacgag ttgaccggtg
     3601 gcgaaggcga cgattggctt gatggccgtg gtggccaggg tcagttgatg ttcggcgact
     3661 ccggcgctcc aagcggcgga caaccgcttt acagcggcaa cgacgtcatg atcggtggcg
     3721 caaacggcgg cgacatcttc aaaggcttca gcggcgacga catcatggtc ggcaacggaa
     3781 gcttcaccaa gttcatcggc ggccttggct tcgactgggg ctcctatgaa accgccatcc
     3841 acggcgttag cgaggacatg aaccggaagg agttcatcac gccaagcggc ctcgccgaag
     3901 actc
//
LOCUS       DASWQN010000785         6200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_999802, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000785 DASWQN010000000
VERSION     DASWQN010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999802"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="VGC82_13245"
     CDS             <1..1377
                     /locus_tag="VGC82_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0424280.1"
                     /translation="GNLRCIGSTTYKEFRNYFEKDRALVRRFQKIDVNEPSVEDSVKI
                     LRGLKLNYEKHHKVRYTDEAIRAAVELSAKYIHDRKLPDKAIDVIDEVGASRMLLPEH
                     KRRKTVTLKDVEEIVAKIARIPPKSVSADDKETLRNLERDLKSMVFGQDKAIDSLAAA
                     IKLARAGLREPEKPIGNYLFSGPTGVGKTEVARQLASTMGIELIRFDMSEYMERHSVS
                     RLIGAPPGYVGFDQGGMLTDAIDQHPHAVLLLDEIEKAHPDLFNILLQVMDHGKLTDH
                     NGKTVDFRNIILIMTTNAGASDMAKEAIGFGREVRTGEDDDAIKRLFTPEFRNRLDAT
                     ITFAGLTPEIVGRVVEKFVMQLEAQLADRNVTIELSSAAKEWLAERGYDRLYGARPLG
                     RVIQEHIKKPLAEELLFGKLVKGGAVKVTLKDNKLEFEFTEAALPALPKPEGDDDSGA
                     GREEEVEV"
     gene            complement(1515..2144)
                     /locus_tag="VGC82_13250"
     CDS             complement(1515..2144)
                     /locus_tag="VGC82_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424281.1"
                     /translation="MADPIPHPAHSYDIKDTTTGVAWQADGTPYDGPVAGLTNEIILG
                     GTDNINVTSYQPNSFIHTGDGQDGIDVSPAGGNNIIDAGTNSNFLTGGSGNDTFYLDG
                     RNPISNIWSTIKGFHLGDHATIWGVTANDFKLTWLQDQGADGARGLTGVFEADGSPKI
                     AVTLSGFGFAGLRDNTIRLSYGRTADLPGLPGSNFATIALASSPFAPTS"
     gene            complement(2689..3387)
                     /locus_tag="VGC82_13255"
     CDS             complement(2689..3387)
                     /locus_tag="VGC82_13255"
                     /inference="COORDINATES: protein motif:HMM:NF006035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0424282.1"
                     /translation="MADHRLALIIGANRGIGLGVTKEFLARGWDVIATARDPAKAVAL
                     QALEAAHSGRVSIVRLEMTDPAALDGFANSLGSKVVDAVLINAGISGPEHHSASAATA
                     AEIGELMFTNAIAPIRLTRSLVSHVRRDTGVVAFTSSIMGSIAANNGGHELYRASKAA
                     LNSLARGLWSELRSRKLTMVSLHPGWVRTDMGGPSAAVSVEDSARGIVTVIEQQSGAH
                     QHRFLDYQGLELPW"
     gene            complement(3554..4753)
                     /locus_tag="VGC82_13260"
     CDS             complement(3554..4753)
                     /locus_tag="VGC82_13260"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424283.1"
                     /translation="MHKLTRRTLLSSTAALAALPARAADAPGITASEILIGQTIPYSG
                     PASSYGTIGKASDAYFHMINDQGGIAGRRLRLLSLDDGYLPPKTVELTRRLIEEDKVA
                     FLFNTLGTPTTNATRKYVNSKKVPQLFIASGATQFGDREHYPWSIGWQPTYQIEGHIY
                     AKYTLQQLPGAKAAVLYQSDDSGRDYFKGFKDGLGAAADTTIVATATYEVTDPTVDSQ
                     ILTLRDKGADVLFMTGIPKFNAMAIRKAFDIGWKPKLFFISSTGSSVATAIMPAGPEK
                     AIDVITASYVKDPTDKQWAKDEGYLAWREWMEKYYPGADVRDVNNVYGYCTAQTLVQT
                     LKQCGSDLSRENIMRQATNLDIALPMLQPGIRVKTGPEDYYPLKQMRLVKFDGSGWTP
                     FGDVISG"
     gene            complement(4808..5794)
                     /locus_tag="VGC82_13265"
     CDS             complement(4808..5794)
                     /locus_tag="VGC82_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HEY0424284.1"
                     /translation="MRAVWYDRQGPANEVIETGEVPTPEPGIGQVRVRLEASGVNPSD
                     TYRRRGAVPAEYARVIPNSDGAGVIDKAGDGVPARWIGKRVWLYNGQRNGRWMGTAAE
                     FIALDVDLVAELPDHVSFAEGATLGIPGMTAHGCVFAAGSVQGKTVLVTGGAGAVGHY
                     AVQLAAWAGAEVIATVSSGDKADRARAGGAAHVVNYRTEDVASRVAEITRGAGVDHVV
                     DVDLGGNLAATLGSVRENGSIAYYASNGALEPKVNLRALMAKNLTVRGFVLPSSPHAA
                     RKRAQLDIAAFIRTRGRVLSVAGEFPLYQTAAAHMSVEAGGKVGTVVVACAR"
     gene            5949..>6200
                     /locus_tag="VGC82_13270"
     CDS             5949..>6200
                     /locus_tag="VGC82_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424285.1"
                     /translation="MAFVGDYDGSIVAVLEQLRLTADLEGLAILDTSPDAADAPIAYC
                     LGVAGPGTIERGQALLRECPGRPSHIVAPDRRPVLACPWV"
BASE COUNT         1169 a         1924 c         1909 g         1198 t
ORIGIN      
        1 ggtaacctgc gctgtatcgg cagcacgacc tacaaggaat tccgcaatta cttcgagaag
       61 gaccgcgccc tggtccggcg gttccagaag atcgacgtga acgagccgtc ggtggaagac
      121 agcgtgaaaa tcctgcgcgg cctgaagctg aactatgaga agcaccacaa ggtccgctac
      181 acggatgagg caatccgcgc cgcggtggag ctttcggcca aatacatcca cgaccgcaag
      241 ctcccggaca aggcgatcga cgtgatcgat gaggtcggtg cgtcccggat gctgttgccg
      301 gagcataagc gccgtaagac cgtcactttg aaagacgtgg aagagatcgt ggcgaagatc
      361 gcccgcatcc ctcccaagtc cgtctcggcc gacgacaagg aaacgctgcg gaacctggag
      421 cgcgacctga aatcgatggt atttggccag gacaaggcga tcgactcgct cgccgccgcg
      481 atcaagctgg cccgagcggg actgcgcgaa ccggagaagc cgatcgggaa ctatctgttc
      541 tccggcccaa ccggcgtcgg caagacggaa gttgcgcggc agttggcctc gaccatgggc
      601 atcgagctga tccgcttcga catgtccgaa tacatggagc ggcattcggt ctcgcgtctg
      661 atcggcgcgc ccccgggata tgtcgggttc gaccagggcg gcatgctgac cgatgcgatc
      721 gaccagcatc cccatgcggt gttgctgctc gatgaaatcg agaaagctca tccggacctg
      781 ttcaacatcc tgctgcaggt gatggaccac gggaagctga ccgaccacaa cggaaagacg
      841 gtggatttcc gcaacatcat cctgatcatg acgaccaacg cgggcgcgtc cgacatggcg
      901 aaggaagcca tcggcttcgg ccgggaggtc cgcactggtg aggatgacga tgcgatcaag
      961 cgcttgttca cgccggaatt ccgcaaccgt ctggatgcga ccatcacgtt cgccggcctg
     1021 acgccggaga tcgtgggccg cgtggtcgag aagttcgtga tgcagctgga agcccagctc
     1081 gccgatcgca acgtgacgat cgagttgtcc tcggcggcca aggagtggct ggcggaacgc
     1141 ggctacgacc ggctgtatgg cgctcggccg ctgggcaggg tgatccagga gcacatcaag
     1201 aagcctctcg cggaggaact gctgttcggc aagttggtca aaggcggcgc tgtgaaggtg
     1261 acgctgaagg acaacaagct ggagttcgaa tttaccgagg cggctttgcc tgctttgccg
     1321 aagccggagg gcgacgacga tagcggtgcg ggacgggaag aagaagtcga ggtttaagta
     1381 ggactgaacg gcaggccccc aagctgactg caccggatct tgggggcctg cgggatgagg
     1441 tccaagacgg tgttatggcg gaaccggcca cgctggcaat gcgatgacca gacccgtggc
     1501 gaattgcgct ggaattagct ggtgggcgcg aacggacttg acgccaacgc tatggtcgca
     1561 aagttgctgc caggcagacc cggaaggtcg gccgttcgac cgtagctaag acggatcgtg
     1621 ttgtcccgaa gcccagcaaa gccaaaccct gagagcgtga ccgcgatctt cggggaaccg
     1681 tccgcttcga atacgccggt taagcccctg gccccatcag cgccctgatc ctgtagccag
     1741 gtgagtttga agtcgttagc cgtcacgccc cagattgtcg catgatcgcc gaggtgaaag
     1801 cccttgatcg tgctccagat attggatatc gggttgcggc catccaagta gaacgtatcg
     1861 ttgccgcttc ctccggtcag gaagttgctg ttggttccag cgtctatgat gttgttccca
     1921 cccgctggcg aaacgtcgat tccgtcctgt ccatcgccag tatggataaa cgaattcggc
     1981 tggtagctcg tgacgttgat attgtccgta cctcccagga tgatctcatt cgtcagcccg
     2041 gcgactgggc catcataagg cgtaccatct gcctgccagg cgacgccggt tgtcgtgtct
     2101 ttaatatcat agctatgcgc cggatgagga attgggtctg ccataacgga acctcttaaa
     2161 tctgaatatt atacacggaa ttttagcgcg tgatcacgct gggcggaacg tcttgagcgt
     2221 gggaattaca cgaacgaaca atgagatatt tcgacctgta ctgtcaggct gaaaatgctt
     2281 taacagacga tcgtgatgcc atcgttagac ggaggacatc aaccgcggcg tcttgatcat
     2341 gtcttgatgg aagcttggca ttcgcgggcc gggtcgctgg tacaggaata atcatgaatg
     2401 attaggacag tgcacgccgc acaaatggaa aataggcaac gcccaagaat gaacggaccc
     2461 aggagcgtga tcacgctgag cggaacgcgt ggatcccgcg atcaaacaag cggatagacc
     2521 atttaaggcc tgcacagtca ggctgaaaat agtctaagcc gataatctcg gggcggaagt
     2581 gtggaccaaa gcacgcacca agtcctcggg agcttgcggc ttcctgcctg gctttgaaac
     2641 catggggcgc cgagtggtgg ccccattgag ccttcaggct gcttcgttct accagggcaa
     2701 ctccaacccc tggtaatcca ggaaccggtg ctggtgcgcg ccgctttgct gctcgatcac
     2761 cgtgacgatc cccctcgcgc tgtcctcgac cgagaccgcg gcgcttgggc cgcccatatc
     2821 cgttcgcacc caaccgggat gcagcgatac catcgtcagc ttccggctgc gcagttcgct
     2881 ccagagtccg cgcgccaacg agttgagagc ggccttgctg gcccgataca actcatgacc
     2941 tccattgttc gccgcgatgc tgcccatgat cgaggacgtg aacgccacaa cccccgtatc
     3001 ccgccttacg tggctcacca gcgatcgagt gagacggata ggggcgattg cgttggtgaa
     3061 cataagttcg ccgatctcgg cggccgttgc tgcgcttgca ctgtgatgct ccggcccgga
     3121 aattccggcg ttgatcagca ctgcgtcaac gaccttgctg cccaggctgt tggcgaaccc
     3181 gtccaatgcc gcgggatccg tcatctccaa ccgaacgatg gacacgcgcc cggagtgcgc
     3241 tgcttccagc gcctgcagtg caacggcctt cgccggatcg cgcgcggtgg cgatgacatc
     3301 ccagccgcgg gccaggaact ccttcgtcac gcccagcccg atgccgcgat tggcaccgat
     3361 tatcagtgca agccgatgat cagccattgt tctatctcca tccagccgaa agcctgttgc
     3421 gtgagactca cggcaccagc ctgaccagca tccatatatc cagcagtcgc aaagcgcgag
     3481 acgcggcgac tgctacgcat catcaggagc ggatttgcct cttgacccgg ccggcgcgct
     3541 ttgctcctgg cggttacccg gagatcacgt cgccgaacgg cgtccaaccg ctgccgtcga
     3601 acttgaccag gcgcatctgc ttgagcggat aataatcctc tggtccggtc ttcacccgga
     3661 tgcccggctg cagcatcggc agcgcgatgt ccagattggt cgcctgccgc atgatattct
     3721 cccgcgacag gtcgcttccg cattgcttca gcgtctgcac cagggtctgc gcggtgcaat
     3781 agccataaac gttgttgacg tccctaacgt ccgcaccggg gtaatatttt tccatccact
     3841 cgcgccacgc caggtagccc tcatccttcg cccactgctt gtcggtggga tcttttacat
     3901 agctggcggt gattacgtcg atcgccttct ccggcccggc cggcatgatc gcggtggcaa
     3961 ccgacgatcc cgtggacgaa atgaagaaca gctttggttt ccagccgatg tcgaaggcct
     4021 tgcggatcgc catcgcgttg aatttcggga tgccggtcat gaacagcacg tcggccccct
     4081 tgtcccgcag cgtcaggatc tgcgagtcga ctgtcgggtc cgttacctca tacgtcgccg
     4141 tcgcgacgat cgtcgtgtcg gcagcggcgc ccaggccgtc cttgaagccc ttgaagtagt
     4201 cgcgccccga gtcgtcgctc tggtaaagca ccgccgcctt ggcccccggc aactgctgca
     4261 gcgtgtattt ggcgtagatg tgaccctcga tctgataggt cggctgccag ccgatgctcc
     4321 aggggtaatg ttcgcggtcg ccgaactggg tggcgccgga ggcaataaaa agctgcggca
     4381 cttttttgct gttgacgtat ttccgcgtgg cattggtggt cggcgtaccg agcgtgttga
     4441 acaggaacgc gactttgtcc tcttcgatca gacgccgggt cagttccacc gtcttcggcg
     4501 gcaggtagcc gtcatccagc gacagcagcc gcaaccggcg gccggcgatg ccgccttgat
     4561 cgttgatcat atggaagtag gcgtcggatg ccttgccgat cgtgccgtac gaggatgccg
     4621 gcccgctgta cgggatcgtc tgcccaatca ggatttccga cgccgttatc cccggcgcat
     4681 cggccgctcg ggctggcagc gcggccagcg ccgcggtgct gctcaacagg gtccggcgcg
     4741 tcagtttgtg catgactgtc ttcctcggga tcatcttccg ctcaacagca caggcccacg
     4801 gaacaagtca acgcgcgcac gccaccacca cggtgccgac cttgccgccc gcctccaccg
     4861 acatatgggc ggccgcggtc tggtagagcg ggaactcacc cgcgaccgac aggacgcgac
     4921 ctcgggtccg gatgaacgcg gcgatgtcca gttgcgcccg cttgcgggcg gcatgcggac
     4981 tgctgggaag gacgaagcct cggacggtta ggttcttcgc catcaacgcg cgcaggttga
     5041 ccttcggctc aagcgccccg ttgctggcgt agtaggcgat gctgccgttc tcccgcacgg
     5101 agcccagtgt cgctgccagg ttgccgccca gatcgacatc gacgacatgg tcaacgccgg
     5161 cgccgcgggt gatctcggcg actcgtgagg caacgtcctc cgtgcggtag ttgacgacat
     5221 gagccgcgcc gccggcccgt gcgcggtctg ccttgtcgcc cgaactgacc gtggcgatca
     5281 cctccgcccc ggcccaggcg gcgagctgca ccgcgtagtg cccgactgcc ccagccccgc
     5341 cggtgacaag cacggtcttg ccttgcaccg aaccggcggc gaacacgcag ccatgcgcgg
     5401 tcatgccggg gatgccaagc gtcgccccct cggcgaagga cacatggtcc ggcagttcgg
     5461 cgacgaggtc cacgtccagc gcgatgaact ccgcggcggt gcccatccag cgcccattgc
     5521 gctggccatt gtacagccag acccgcttgc cgatccacct ggcaggcact ccgtcaccgg
     5581 ctttgtcgat gacgcccgcc ccgtcactgt tcgggatgac ccttgcatac tcggctggta
     5641 cggcaccgcg ccggcggtac gtgtcggatg gattcacgcc cgacgcctcg agccgcaccc
     5701 gcacctggcc gatgccaggt tccggtgtcg gcacctcccc agtctcgatc acctcgttcg
     5761 ctgggccctg acggtcatac caaacggcgc gcatggtttt ctctcctatt ctcgcgctca
     5821 tcgtagccta tttgtccgcg tgttgtgcag ggcggccagc gcggatggca ggttcggccg
     5881 gatttcctat ccatcgcgtc acggaaaagc ctaggctggt tccggtatcg tgacttggag
     5941 agactgacgt ggcttttgtc ggcgattatg acgggtccat cgtcgcggtg ctggagcaac
     6001 tgcgtctgac agctgacctg gaaggtttgg cgatcctgga cacgagcccg gatgcggctg
     6061 atgcgccgat cgcctattgc ctgggcgtcg ccggcccggg cacaatcgaa cgtggtcagg
     6121 ccttgttgcg cgaatgccct ggccgtccgt cccacatcgt tgcaccggat cggcgtccgg
     6181 tactggcctg tccgtgggtc
//
LOCUS       DASWQN010000786         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2056098, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000786 DASWQN010000000
VERSION     DASWQN010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2056098"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VGC82_13275"
     CDS             complement(<1..200)
                     /locus_tag="VGC82_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424286.1"
                     /translation="MFAVLAESPGFRLPCRPMQFLHAADIHLDSPLTGLSSRGSVPLH
                     VVQHCTRRAFANVVDLAIARDVA"
     gene            complement(376..>1697)
                     /locus_tag="VGC82_13280"
     CDS             complement(376..>1697)
                     /locus_tag="VGC82_13280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0424287.1"
                     /translation="GFQHLVRDHRNREAARELPASTVLKLAPERDWVQRRIEQARVAG
                     QLSFTQDEALAVLSAYGIPTVPTRFAASPMDAAAAADLLGYPGVVKLRDTAAPADRLP
                     GSLVFDLHDASQVVAACQKLFARKVRPGGTDELLVQRHAGRGRELAIRVSDGATFGPT
                     ISFGAGGTMPNPEDLAADLPPLNLTLAQGLIRRSRTGAMLTEPLRDRPPANAAAVAEV
                     LVRISQLIVDFPEIAVLDVPSLFADADGVLAADAWLRLRGLQEPLVRLAIAPYPSELT
                     EHRQVGGERITIRPIRPEDAQAHAAFFSRLSPQDIRYRFFSAMRELSAEQTVRLTQVD
                     YDREMAFLAVREATNETVGVCRLACDADGRSGEFAVIVQADMKGRGLASHLMHRLIDW
                     ARSRGLQQIEGQILADNQPMLAFIRHLGFSLRRMPDDPEVMEAQLWL"
BASE COUNT          325 a          552 c          541 g          279 t
ORIGIN      
        1 gccacatccc gggcgatcgc caggtccacg acgttggcga acgctcggcg ggtgcaatgc
       61 tgaacaacgt gcagagggac gctgccgcgg ctggaaaggc cggtcagcgg gctgtccagg
      121 tggatgtcgg cggcgtggag gaactgcata gggcggcagg gtagccgaaa gccaggcgat
      181 tcggccagaa ccgcgaacat gattgcacca ggttagggtg gtgatggtgg cggtcatagt
      241 gctgtggtcg gttgaaaata taaccgcttc aagcgcgatc tcagatccgt catggtcaac
      301 gaaggccgca gccttgattg atagtgtcat ggccgtcgat gatagtgatg ccgaccacga
      361 catcgcatag ccggatcaca accaaagctg ggcctccatc acttcgggat cgtccggcat
      421 gcggcgcagc gaaaaaccca ggtgccggat gaacgccagc atcggctgat tgtccgccaa
      481 tatctggccc tcgatctgtt gcagcccgcg tgaccgcgcc caatcgatca gacggtgcat
      541 caagtgcgac gcgaggcccc ggcctttcat gtccgcctgc acgatcaccg cgaactcccc
      601 ggaccgtcca tcggcatcgc aggccagacg gcaaaccccc accgtctcgt tggtcgcctc
      661 ccgcaccgcc aggaatgcca tttccctgtc gtagtccacc tgcgtcagcc gaaccgtctg
      721 ttcggctgaa agttcgcgca tagcgctgaa aaaccgataa cggatatcct gcggcgacag
      781 gcgggagaag aaggccgcat gcgcctgggc atcctccggc cttatcggcc tgatcgttat
      841 ccgttcccca ccgacctgcc gatgttcggt caactccgaa ggatacggtg cgatggccag
      901 acgaacgagc ggctcttgca gcccgcgcag tcgcagccat gcgtcggcgg ccagcacgcc
      961 gtcagcgtcg gcgaacaagg atgggacatc cagcactgcg atttcgggga agtccacgat
     1021 caactgactg atccgcacca gcacctcggc gacggcggcg gcgtttgccg gcgggcggtc
     1081 ccgaagaggc tccgtcaaca tcgccccggt gcggctgcgg cggatcaggc cttgcgccaa
     1141 cgtgaggttc aagggcggca gatcggctgc gagatcctcg ggattcggca tcgtgccgcc
     1201 ggcgccgaat gaaatggtgg ggccaaacgt agcaccgtcg gacacgcgga tcgccagttc
     1261 ccgcccgcgt cccgcatggc gctgaaccag caattcgtcc gtcccgccag gccggacctt
     1321 gcgggcgaac agcttttggc aggccgcgac aacctgcgag gcgtcgtgca gatcgaatac
     1381 caggcttccg ggcaagcgat cggccggcgc tgccgtatca cgcagcttga ccactccggg
     1441 atagcccagc agatccgcgg ccgccgccgc atccatcgga ctggcggcga agcgggtggg
     1501 cacggttgga attccatagg cactcagaac cgccaacgct tcatcctgcg tgaacgacag
     1561 ttgtccagcg acacgcgcct gctcgatgcg gcgctggacc cagtcgcgtt cgggcgccaa
     1621 cttcaacacc gtgctggctg gcagttcgcg cgccgcctca cggttgcggt ggtctcgaac
     1681 cagatgctgg aagcccc
//
LOCUS       DASWQN010000787         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1792210, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000787 DASWQN010000000
VERSION     DASWQN010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792210"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1243)
                     /locus_tag="VGC82_13285"
     CDS             complement(20..1243)
                     /locus_tag="VGC82_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY0424288.1"
                     /translation="MLKQDIVHEATRLTRAGQLVEATALLQRMLLGESAPDATSRTTG
                     RIALTGREPPTIDANANAIEETDSPHLARATSAQPRMLRALLDRTKERSGLGLQGVIK
                     RAPLSTPDIVPEGARFIEGTYSNPAGSRAYRLFIPSRYQGQPLPLVVMLHGCTQSPDD
                     FATGTRMNFIAEEQTCFVVYPAQRSEANQAKCWNWFRTADQQRGRGEPSLIAGITRQI
                     MRDYSVDSKRVYVGGLSAGAAAAAVMGATYSDLYAAIGVHSGLACGAAIDLPSAFVAM
                     RQGGGSDDRVILGDGPAVPTIVFHGDRDITVHPNNGDQILEQSMRTTSTQKKVHRGRV
                     PGGHAYTRTIHTDASGRGVFEHWNIHGAGHAWSGGSPAGSYTDPRGPDATREMLRFFL
                     EHSLPSSEASVSLHP"
     gene            complement(1249..1668)
                     /locus_tag="VGC82_13290"
     CDS             complement(1249..1668)
                     /locus_tag="VGC82_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY0424289.1"
                     /translation="MADNVQELRPKPADTEKITINLGYVDLGHVDLMVQEGFYSNRTD
                     FIRTAIRNQLERHADVVKQSTVRKSLDLGLRNYSREDLEAARRAGEMLRINVLGLASI
                     AQDVTPELARATIASVSVLGALHASPAVKAALADRTR"
BASE COUNT          337 a          520 c          552 g          381 t
ORIGIN      
        1 taagcaagcc gcggaaaacc tacgggtgaa gtgacacgct agcctcactg ctggggagcg
       61 aatgctcgag gaagaaccgc agcatttccc tcgttgcgtc cggtcctcgc ggatcagtgt
      121 aggagcccgc agggctgcct cccgaccatg cgtgtccagc tccgtggatg ttccagtgct
      181 caaagactcc gcgcccgctg gcgtcggtat ggatcgtgcg ggtataggca tgccctccag
      241 gtacccgccc gcgatgcacc ttcttttgcg tgctcgtcgt tctcatagac tgctcaagaa
      301 tttgatcacc gttgtttgga tgcaccgtaa tgtcgcgatc gccgtgaaaa acaatggtcg
      361 ggacagctgg cccgtcacct agaattaccc tgtcatcgga cccgccacct tgtcgcatgg
      421 cgacaaacgc agagggaagg tcaatggcgg ccccacaggc gaggccagaa tgtacgccga
      481 ttgccgcgta cagatcgctg tacgttgctc ccatgacagc agcagcggcc gctccggccg
      541 acagtccacc aacgtagacg cgctttgaat caaccgaata gtcgcgcatg atttggcgag
      601 tgatgcccgc gataagcgaa ggttcacccc tgccccgctg ctggtcggct gtgcgaaacc
      661 agttccagca tttcgcctgg tttgcctcgc tgcgctgtgc aggatagacc acaaagcaag
      721 tttgttcttc tgcgataaag ttcatcctcg taccggtggc gaaatcgtct ggcgactgcg
      781 tgcagccgtg tagcatgacg accaaaggaa gcggttgccc ctgataacgg ctggggatga
      841 agagcctgta ggcacggctt cccgccggat tgctgtaggt accttcgatg aaccttgcgc
      901 cctccggcac gatgtccggc gtggatagcg gggcgcgctt gatcaccccc tgcagtccga
      961 gcccagagcg ctcctttgtg cgatcgagca acgcgcggag catgcgcggt tgagcggatg
     1021 tggcccgcgc taggtgcgga ctatccgtct cctcaatagc attggcgttc gcgtcaatgg
     1081 ttggcggctc gcgtccggta agcgcgatgc gaccggtggt gcgcgatgtc gcgtctggcg
     1141 cgctctcacc gagaagcatg cgctgcagga gcgcggtggc ctcgacgagt tggcccgcgc
     1201 gcgtgaggcg tgtggcttcg tgaactatgt cctgtttcag catcgccttc accgcgtcct
     1261 gtcggcaaga gcggccttga ccgccggact ggcatgcaaa gcccccagca cggagactga
     1321 ggcaattgtg gcgcgagcaa gctcgggcgt gacgtcttgg gcgatactgg caaggcccag
     1381 aacattgatg cgaagcatct caccggctcg ccgcgccgct tcgagatcct cgcggctgta
     1441 gttccgcaga ccgaggtcca ggctttttcg gaccgtggac tgcttgacta cgtccgcgtg
     1501 acgttcgagt tgattgcgga ttgccgttcg aatgaaatcg gtacggttcg aatagaaccc
     1561 ttcctgcacc attaaatcga cgtgaccaag gtcgacataa ccaaggttga tcgtgatttt
     1621 ttcggtgtcg gcgggctttg gtcggagttc ttggacattg tcggccatga ttaccatcca
     1681 ttaaacatct gtgtggatgg tatatggatg tcaagaagcg cctgtcaagg gtacgcgtcc
     1741 ggtcattggc tagatcgctt ctggcgcaca gcgaaggccg gcgagttgtc
//
LOCUS       DASWQN010000788         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1660202, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000788 DASWQN010000000
VERSION     DASWQN010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660202"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..249)
                     /locus_tag="VGC82_13295"
     CDS             complement(49..249)
                     /locus_tag="VGC82_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424290.1"
                     /translation="MMSHDRSPEPRSTGELPVIVACIIFLLHLMPGGLLAGLVLWTLA
                     SFPIGVLIGHCILSEEAKPHVQ"
     gene            complement(444..884)
                     /locus_tag="VGC82_13300"
     CDS             complement(444..884)
                     /locus_tag="VGC82_13300"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEY0424291.1"
                     /translation="MKMMRLGIAIGLLAGLGACSDMMGGKSSGTPRAVATQPTVAPDM
                     VRQVQAKLRDGGYYKQGSVDGVWGSGTEAAVRSFQQDHNMSASGQLDVPTLQALNLAG
                     GPPASSTAATNNPANIDTRPTQPMRTDANVNSPPTDNTAAPPPR"
     gene            complement(983..>2023)
                     /locus_tag="VGC82_13305"
     CDS             complement(983..>2023)
                     /locus_tag="VGC82_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY0424292.1"
                     /translation="EVLNAMVESGVITADQAQAAASRIAFPPSPTAPGWFAAWVADQS
                     QALLAPDVDAVLRTTLDNRQQAVAEIRLSALLDGPGAAADVGQGAVVIIDAASGAVRA
                     MVGGRNFRESPFNRAVLARRQPGSVFKPFVWLAALEHGMTPDDTVLDAPLRIGSWSPV
                     NFDHRYLGSVTLEEALAQSINTASVRLLIQAGGARTVAATAARLGIADKLPNDASLAL
                     GTGEVGLLELTAAYAPFFNGGNRVIPHGLDRTPHQPVPVIEPRQAASMARMLASVVSR
                     GTGRAAAVPGRGVAGKTGTTQDSRDAWFIGSVRGMLIGIWLGNDDNQPMKGILGGGLP
                     ARLFHDIAMGID"
BASE COUNT          356 a          695 c          614 g          358 t
ORIGIN      
        1 cttcggcgat acgcggttct gccgggaccg ttacgcccaa gtgatgggct actgaacgtg
       61 cggctttgct tcttcgctta atatgcaatg gccgatcagg acgccgatcg gaaaggacgc
      121 caacgtccaa agaacgagcc ctgccagcag tccgcccggc atcaggtgca gcagaaagat
      181 gatgcaggcg acgatcaccg gcaattcccc ggtggagcgc ggttcgggtg acctatcgtg
      241 gctcatcatg gcctaaatct ccagttcggg cgcttttcgt tctgatacag aaacactatg
      301 atgcacaccg cgtgccgcgg aaagatcgct gttgaatcag cgcgctgaga catgattcca
      361 gcacttgcgc gagagagtgt aaatagttcc gacagccgcc atccggccga caggtcccgg
      421 aattaccggg acccgggcca gcttcaacgt ggtggaggtg ctgcggtatt atccgtcggc
      481 ggcgaattga cgttagcgtc cgtgcgcatc ggctgcgtcg gcctcgtatc gatgttcgcc
      541 gggttgttcg ttgcggcggt gctgcttgcc ggtggtccgc ctgccagatt cagcgcctgc
      601 agggttggca catccaactg tccactcgcg ctcatgttgt ggtcttgctg gaacgaccgt
      661 acggccgcct cggtgcccga tccccagacg ccgtcaaccg aaccctgctt gtagtagccg
      721 ccgtcgcgca gcttcgcctg aacctgccgc accatgtccg gcgctaccgt tggttgcgtc
      781 gccaccgcgc gcggtgttcc actcgatttg ccgcccatca tgtccgaaca ggcgcccagc
      841 ccggccagca ggccaatcgc gatcccaagg cgcatcatct tcatcggttc ctccatcgtt
      901 ttggcacagt ggaagcggcc ggtcggcggt ttggttccaa acgccacgga tagcgaagcg
      961 gagaggtggt gacgcgccgg tttcagtcga tgcccattgc gatgtcatgg aacaatcgcg
     1021 ccggcaatcc accgccgagg atgcccttca tcggctggtt gtcgtcattg cccagccaga
     1081 taccgatcag cattcccctc accgatccga tgaaccaggc atcgcgcgag tcctgcgtcg
     1141 tgccggtctt gcctgccacc ccacggcccg gtacggccgc cgctcgcccg gtgcctcgac
     1201 tgacgaccga agccagcatg cgcgccatgc tcgctgcttg gcgcggctct ataaccggca
     1261 cgggctggtg cggcgtgcga tccaggccgt gcggaataac ccggttgccg ccattgaaga
     1321 acggcgcata ggcggccgtc agctccagca gcccgacttc cccggttccc agcgccagcg
     1381 aggcatcgtt cggcagctta tcggcgatgc ccaaccgggc cgccgtggcg gcgaccgtcc
     1441 gcgcacctcc agcctggatc aagagccgga ccgaggcggt gttgatcgac tgcgccagcg
     1501 cctcctccag cgttaccgaa cccagatacc ggtggtcgaa gttgaccggg ctccaactgc
     1561 cgatgcgtag cggcgcgtcc agcaccgtgt cgtcgggggt catgccgtgc tccagcgccg
     1621 ccagccaaac gaacggctta aacaccgaac ccggctgccg ccgcgccaaa accgcacggt
     1681 tgaacggact ttcgcgaaaa ttccgcccgc cgaccatggc tcgcaccgcg cccgacgccg
     1741 catcgatgat gacgaccgcg ccctggccca cgtcggccgc cgcaccggga ccatccagca
     1801 gtgccgaaag ccggatttcc gccactgcct gctggcggtt gtccagggtt gtacgcagca
     1861 ccgcatcgac atccggagcc agcagtgcct gcgactgatc ggccacccag gcggcgaacc
     1921 acccgggcgc ggtggggctg ggcggaaaag caatccgcga agccgccgcc tgcgcctgat
     1981 cggcggtgat aactccagac tccaccatcg cattcagcac ctc
//
LOCUS       DASWQN010000789         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1131568, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000789 DASWQN010000000
VERSION     DASWQN010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131568"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="VGC82_13310"
     CDS             <1..1584
                     /locus_tag="VGC82_13310"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0424293.1"
                     /translation="VRPSRFRVAPNRVAPRLVTRAGIQTIEALIEFETAIGRGNGVLR
                     LVPDAEGKLAAWVLLTALDEIKGHEERIGARASTGQAFSREFGGPNWLDYRRKAQAYE
                     EHDPVAVVIGGGQAGLGIAACLGRLDVDTLVIDRHERIGDAWRKRYHSLTLHNEVHIN
                     HLPYMPFPPNTPVFIPKDKLANWFESYADAMELNVWTGTELKHGVFDETTGTWEVSLR
                     RQDGSERILRPRHLIFATGVSAIPVRPNAPGLDDFAGTVMHSGQYTTGHAWKGRKALV
                     MGTGNSGHDVAQDLHASGANVTMIQRSTTLIVSLEQAQKVYDMYKEGPSLADCDLLAT
                     AVPYKVLVKGYQIAAAAMQAADKPLLDGLAARGFRLDNGPPDDTGFQMKYLRRGGGYY
                     FNVGCSDLIVDGQIGLLQYSDIDRFVPEGVRMKDGRVIEADLLVAATGYKNQQDVVRA
                     YLGDEVADRIGPVWGFDEGGEERNMWRRTAQPGLWFTAGGLPHVRIYSKYLALQIKAL
                     EEGLLGASNAVREGALQES"
     gene            1675..>1862
                     /locus_tag="VGC82_13315"
     CDS             1675..>1862
                     /locus_tag="VGC82_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019087450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HEY0424294.1"
                     /translation="MTIRNVAPATKERLRVRAASHGRSMEAELRAILNAALESGPDRE
                     VNLAEAIRRRFAPVGGVDL"
BASE COUNT          355 a          531 c          662 g          314 t
ORIGIN      
        1 gtacggccaa gcaggttccg tgtcgctccg aaccgggtgg cgcccaggct ggtgactcgc
       61 gccggaatac aaacgatcga ggcgttgata gagtttgaaa cggcgatcgg ccgcggaaat
      121 ggggtgttgc ggctggtgcc cgatgcggag gggaaactcg ccgcctgggt gctgttgacg
      181 gcgttggacg aaatcaaggg ccacgaggaa cggatcggtg cccgagcttc caccgggcag
      241 gctttttccc gcgagttcgg cggcccgaac tggctggact accgacgcaa agcgcaggct
      301 tacgaggagc acgacccggt tgcggtggtg atcggcggcg ggcaggccgg gttgggcatc
      361 gcggcgtgcc tcgggcggct tgatgtggat acgctggtca tcgaccggca cgaacggatc
      421 ggcgatgcgt ggcgcaagcg gtaccactcg ctgacgctgc acaacgaagt gcatatcaat
      481 cacctgccgt atatgccgtt tccgcccaac acgccggtgt ttatcccaaa ggataagctg
      541 gccaactggt tcgagtccta cgccgatgcg atggagctca acgtctggac cgggacggag
      601 ctgaagcacg gggtcttcga cgaaaccacc ggaacgtggg aagtctcgct acgccggcag
      661 gacgggtcgg aacggatcct gcggccgcgg catctgatct tcgcgacggg ggtcagtgcc
      721 attccggtgc ggccgaatgc acccgggctg gatgattttg ccggcacggt gatgcactcc
      781 gggcaataca ccacgggcca tgcgtggaag ggccgcaagg cgctggtgat ggggaccggg
      841 aacagcggtc acgatgtcgc ccaggacctg catgccagcg gcgcgaacgt gacgatgatc
      901 cagcgcagca cgacgctgat cgtcagcctg gagcaggcgc agaaagtcta tgacatgtac
      961 aaagagggtc catcgctggc ggattgcgac ctgctggcga cggcggtgcc ttacaaggtg
     1021 ctggtcaagg gctatcaaat cgcggcagcg gcgatgcagg ccgccgacaa gccgttgctc
     1081 gacggtttgg ccgctcgggg gttccggctg gacaacgggc ctccggacga taccggcttc
     1141 cagatgaagt atctgcggcg cggcggaggt tattacttca acgtcgggtg ttcagacttg
     1201 atcgtggatg ggcagatcgg gttgctgcag tacagcgaca tcgaccggtt cgttcctgaa
     1261 ggcgtgcgga tgaaggacgg gcgcgtgatc gaggccgatc ttctggtggc ggccaccggg
     1321 tacaagaacc agcaggatgt ggtgcgagcg tatctgggcg atgaggtcgc ggatcgcatc
     1381 gggccggtct ggggcttcga cgagggtggc gaggagcgca atatgtggcg gcgcacggct
     1441 cagcccggac tatggttcac ggcgggcggg ttgccgcatg tgcggatcta ctcgaagtac
     1501 ctggcgctgc agatcaaggc gctcgaggaa ggcttgctgg gcgcgagcaa cgcggtccgg
     1561 gagggcgcgc tgcaggaaag ctgatccggc agccacatcg cgaacaagct tgcagtcaat
     1621 gtatgcattg actacactgg cttcatgcac accgatcaac tccagacgac cacattgacc
     1681 atccgcaacg tggcacccgc caccaaagag cgtctgcgcg tgcgtgcggc cagccacggc
     1741 cgatccatgg aagcggaact gcgcgccatt ctgaatgcgg cacttgaaag cggccccgac
     1801 cgcgaagtca acctggcgga ggcgatccgc cggcgcttcg ccccggtcgg tggcgtcgat
     1861 ct
//
LOCUS       DASWQN010000790         3032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2584635, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000790 DASWQN010000000
VERSION     DASWQN010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2584635"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1773)
                     /locus_tag="VGC82_13320"
     CDS             complement(<1..1773)
                     /locus_tag="VGC82_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424295.1"
                     /translation="MSRTRTSRALAAGAVLVGLTTGGQAATPETAATPETKAPGIIVR
                     SGNHPGFGRIVIGTDAKATYRLDQDGDHVVVVLANDLILAAPPALPRNVAAMTTDGSA
                     LDMVLSHGAQLHAARMDGRIVLDILNTVSPPSSRPSSGPRPGKILSMASSAELGGRSG
                     YGKTGTSPPNIAAVTEPPPVTTKAPASSPPGHPVAGPQVAKMSVANMGVTGEGVSAPA
                     MPRPEPSGVNTLGSDVLGAGAIESAQQVPPGRDVMPENEGPVGLLARRIKLPKGMDGS
                     AFLVPFESTTAAASFRSGDSTYIVFNERRPVDMAALAAEPTFGAASVRLLPSGTVFRI
                     PHPATLSIALTQVPQGWRIAALTATPKRQAIVASQVDGRLNLAAEQPGDVVTMADPDT
                     GATLLVGTQRRAGQAVAAGRQSTEFILRPTIQGVVVEPLSDQIALKQIPTGFSLSGGP
                     SGLRLSPPTSATAALMDASNLTRRLNFPTMAPDALMRRALRQFGEAADSAPLARGPRH
                     HAVAESFLALGLAVEAQSLLHMAVEQDPKEAASATTGALSAVAALLAGRPAEADALLD
                     QRLDGTDEIALWRAVRQAMLDEGSP"
     gene            complement(1770..2423)
                     /locus_tag="VGC82_13325"
     CDS             complement(1770..2423)
                     /locus_tag="VGC82_13325"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HEY0424296.1"
                     /translation="MLDPSSDPAAVGIDALKQKLASIRTNQPQVESAMVTEVVRAVLT
                     TMSHDLTAKEASLLREVEELGRTITSAKSEIAALRVDDITDHDIPFATDELDAIVDHT
                     AHATNAILESCEMLDEVAATVSGEAAAKLQEAVTRIYEACSFQDITGQRITKVVGTLK
                     VIEEKVAQIIGTFGPSIAAPKRGADRVATDADLLNGPQLPAHAMDQSDIDALMASFE"
     gene            complement(2416..2802)
                     /locus_tag="VGC82_13330"
     CDS             complement(2416..2802)
                     /locus_tag="VGC82_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HEY0424297.1"
                     /translation="MAIDKSMNVLIVDDYKTMLRIIRNLLKQIDFNNVEEASDGAEAL
                     SKLRGGNFGLVISDWNMAPMTGLQLLQEVRADNRLKTLPFIMITAESKTENVVAAKAA
                     GVSNYIVKPFNAETLKGKIEKVLGHA"
     gene            complement(2828..>3032)
                     /locus_tag="VGC82_13335"
     CDS             complement(2828..>3032)
                     /locus_tag="VGC82_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424298.1"
                     /translation="AAIFNDLSMPVMLQVLDRMKDAKAASVMAAMNPEKARDVTAELA
                     QLRTGRATVPPSPAAPRPVSAGS"
BASE COUNT          475 a          955 c         1002 g          600 t
ORIGIN      
        1 gggggaacct tcatccagca ttgcctgccg caccgcgcgc cacagagcga tctcgtcggt
       61 gccgtccagc cgttggtcca acagcgcgtc ggcttcggca ggccgtccgg ccagcaacgc
      121 ggcgacggcg gacagggcgc ctgtcgtggc cgatgcggcc tctttcggat cctgctccac
      181 ggccatgtgc aacaggctct gagcctcgac ggcgagaccg agtgccagaa agctttctgc
      241 gacggcgtgg tgtctggggc cgcgcgccaa gggggccgaa tcggccgcct cgccgaactg
      301 ccggagtgcg cggcgcatga gcgcatcggg tgccatcgtt ggaaaattca ggcgcctggt
      361 caggttcgat gcgtccatca gtgcagccgt ggcgcttgtc ggaggggaca gcctgaggcc
      421 ggaggggccg cccgacaggc tgaatccggt tgggatctgc ttcagcgcga tctgatccga
      481 tagcggctcg acaaccacgc cctgtattgt cggccgcagg atgaactccg tgctctgccg
      541 gcctgcggcc accgcctggc cggcgcggcg ctgggtgccg acaagcaacg tggccccggt
      601 atcgggatcc gccatggtga cgacatcgcc cggctgttcc gcggccaggt tgagacgtcc
      661 gtcaacctgg gacgcgacaa ttgcttgccg ctttggcgtc gccgtcaggg ccgcgatccg
      721 ccagccttgt ggcacctgcg tcagggcgat cgacagcgtg gctgggtggg ggattcgaaa
      781 cacggtgccg ctcggcagga ggcgcaccga cgccgcgccg aacgtgggtt cggctgccaa
      841 cgcggccata tcgacggggc gacgctcgtt gaaaacgatg taggtgctgt cgccgctccg
      901 gaacgaggcg gcggcggtgg tgctttcgaa cggcaccagg aaagcggatc cgtccatgcc
      961 ctttggcagc ttgatccgcc gggccagcag gccgaccggg ccctcgttct ccggcatcac
     1021 atcgcgccct ggcggcacct gctgagcgga ctctatcgcg cctgccccga gcacatccga
     1081 accgagtgta ttcacgccag atggttcagg tctgggcatg gctggagcgg agacgccttc
     1141 gcccgtaacg cccatgttgg caacgctcat cttggcaacc tgtgggccgg ccaccgggtg
     1201 acctggcggc gatgacgccg gcgccttggt cgtgactggt gggggctccg ttaccgccgc
     1261 gatgtttggc gggctcgtgc cggtcttgcc gtagccggac cggccaccca gttccgccga
     1321 cgacgccatt gaaaggattt tgcccggacg tggtccggac gagggtcggc tactgggcgg
     1381 cgaaacggtg ttcaggatgt ccaggacgat ccggccgtcc atgcgcgcgg catgcaattg
     1441 agcaccatgg ctcaggacca tgtcgagggc tgaaccatcc gtcgtcatcg cggcgacatt
     1501 ccgcggcaat gccggtgggg ccgcgaggat aaggtcgttc gccaggacga caaccacatg
     1561 gtcgccgtcc tgatcgagcc ggtaggtggc ttttgcatcc gttccgatca cgatccggcc
     1621 gaagccgggg tggttaccgg atcgaacgat gatgccgggt gcttttgttt ccggcgtagc
     1681 ggctgtctcc ggcgtagcag cctggccgcc ggtggtcagg ccgaccagga cagcgccggc
     1741 ggcaagtgcc cgcgatgtcc tggtgcggct cattcgaagc tcgccatcag cgcgtcgata
     1801 tccgactggt ccatcgcgtg cgccggaagc tggggaccat tcagcagatc cgcgtcggtg
     1861 gcgacacgat ccgcgccgcg cttcggtgcc gcgatggatg gtccgaaggt gccgatgatc
     1921 tgtgcgactt tctcctcgat gaccttcaac gtgccgacca ccttggtaat ccgctggccg
     1981 gtgatgtcct gaaagctgca ggcttcgtag atccgggtga ccgcttcctg caattttgcc
     2041 gccgcttcgc cactaaccgt cgcggcgact tcgtccagca tctcgcagct ttccaggatc
     2101 gcgttcgtgg catgcgccgt atgatcgacg atcgcatcga gctcatccgt ggcgaaaggg
     2161 atgtcatgat ccgtgatgtc gtccacgcgc agcgcggcga tctccgactt ggcggacgtg
     2221 atggtccggc caagttcttc cacctcccgc agcagcgagg cttccttcgc ggtcaggtcg
     2281 tggctcatgg ttgtcagcac ggctcgtact acctcggtga ccattgcgga ttcgacctgg
     2341 ggctggttcg tgcggatcga agccagtttc tgtttcagtg catcgatgcc gacggcggca
     2401 ggatcgctgc tgggatcaag catgacccag gactttttca atcttgccct tgagcgtttc
     2461 ggcgttgaat ggcttgacga tatagttcga aacgccagcc gctttggcgg cgacgacgtt
     2521 ttccgtcttg ctttcggcgg tgatcatgat gaacggcagc gttttcaggc gattgtccgc
     2581 acgcacttcc tgcagcagtt gcagcccggt catcggggcc atattccagt cgctgatcac
     2641 caggccgaag ttgccgcccc gcagtttgga cagcgcctcc gcgccgtccg aggcttcctc
     2701 gacattgttg aagtcgatct gcttgagcag gttgcggatg atccgcagca tcgtcttgta
     2761 atcatcgacg ataagcacgt tcatggattt gtcgatcgcc atcttttact ctccgttcgt
     2821 ggttgaatta gctgcccgcg gaaacaggtc gcggcgcggc aggcgatggc ggaaccgtgg
     2881 cgcggccggt ccgcaactgc gccagttccg ccgttacgtc gcgggccttc tcggggttca
     2941 tcgccgccat gactgaagcg gccttcgcat ccttcatccg gtcgagcact tgcagcatca
     3001 ccggcatcga aagatcgttg aagattgccg cg
//
LOCUS       DASWQN010000791         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2451563, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000791 DASWQN010000000
VERSION     DASWQN010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2451563"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            381..1118
                     /locus_tag="VGC82_13340"
     CDS             381..1118
                     /locus_tag="VGC82_13340"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0424299.1"
                     /translation="MLLRRLMNEPPMQLAKGEFGGRVGIWRLIELFDTHGVKATIFTP
                     GRICELYPQAIRAAARSGHEVADHMWEHHVPQDAALEQDHLRKTVAALERLTGKRPVG
                     TRSSHSLPLLRDEGFIYVSENAGDHLPYYVRDQSRQVCLLNLPFHFAIDDAMFFSFAW
                     MGSGNAAQRITDPDRVLDLWWDAFMHQYRQGGYLNVCLHPFVSGRALRITMLDRLITR
                     MKTLPGVWFPSCEELARHILKTTPPNT"
     gene            1128..>1747
                     /locus_tag="VGC82_13345"
     CDS             1128..>1747
                     /locus_tag="VGC82_13345"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0424300.1"
                     /translation="MLWKQGYTISDEISLSDKDLHWPDGNRCCVSITVNLSVARSPEG
                     IRASDLQTADAFFALNDGLDQVLSLLHRHRFKATFVVPAVLADIRPGLLRDLVAQGHE
                     IAAGGFRHEDVSHLPRDEAAARIAAATDILTRVTGQRPEGWYSLPRPTDAFANGTISP
                     YTMDLLIDAGYTWFGNGLADDIPHYWVTDFAARRAILTLPYYYHFD"
BASE COUNT          327 a          578 c          515 g          327 t
ORIGIN      
        1 ggaacgaatg gcgccaatcg ccgcaggctg gttgccgtcc gcccaggtcc gtccccccta
       61 tccgcagcaa cgcttcatcg tccaattccc aaggtgggag ccgagtgcgt tggcagcgca
      121 ctctcggatc tgtccggggg gcgcggggta acccgcgttc taccggatgg caagtctgcg
      181 acggtggacc gtatcggcct tcgaggttag cattctctcc gagtgaagga ccggcacgta
      241 tggacatcga attgtcacgc gacatcgcgg cgatctccgg tgccgccgca gcatatgccg
      301 ctgcgcacag gcgcatcgcc aggttggttt atccaacggg catacagctg gccgtcaact
      361 tcaccgcgga cttcgacacg atgctgctgc gtcggctgat gaacgaacca ccgatgcagc
      421 ttgccaaggg cgagttcggc gggagggtcg gcatctggcg gctgatcgag ctgttcgata
      481 cccatggggt aaaggcaaca atcttcacgc ccgggcgcat ctgtgagctg tatccccagg
      541 cgatacgcgc ggcagcccgc agcgggcacg aagttgccga ccacatgtgg gagcaccatg
      601 taccgcaaga tgccgctctc gaacaggacc acctgcgcaa gactgttgcg gcgctggaac
      661 ggctgaccgg gaagcgaccg gtcggcaccc gcagttcgca cagcctgccg ttgctacgtg
      721 atgagggctt catctatgtc tcggagaacg ctggcgatca cctgccgtac tacgtgcgcg
      781 accagtcgcg gcaggtctgc ctgctgaacc tgccattcca cttcgccatc gatgatgcaa
      841 tgttcttcag cttcgcctgg atgggcagtg gcaacgcggc gcaacgcatc accgaccccg
      901 atcgcgtcct cgatctttgg tgggatgcat tcatgcacca atatcgtcag ggcggctacc
      961 tcaacgtctg cctccatccg ttcgtttccg gacgagcctt gcgcatcacc atgctcgatc
     1021 ggctcatcac ccggatgaaa acacttccgg gggtctggtt cccaagttgt gaggaactgg
     1081 cacgccacat cctcaaaacg accccaccca acacgtaagg ccgcccgatg ctctggaagc
     1141 aaggctacac gatttcggac gaaatcagcc tgtcggacaa ggacctgcac tggcccgacg
     1201 gcaaccgctg ctgcgtcagc atcacggtca atctcagtgt ggcgcgcagc cccgaaggga
     1261 tccgcgccag cgacctgcaa acagccgacg cattcttcgc gctgaacgac ggtctggatc
     1321 aggtcctgtc tctgctgcac cggcaccggt tcaaggccac gttcgtggtg ccggctgtgc
     1381 tggccgacat tcgcccaggc ctgttgcggg acctcgttgc gcaaggtcac gagattgccg
     1441 ctggcggctt tcgccacgaa gacgtcagcc accttccgcg tgacgaggcg gcggcccgca
     1501 tcgccgccgc caccgacatc ctgacacgcg tcaccggtca acgtccggaa ggctggtact
     1561 ctctgccacg cccgactgac gccttcgcca acggcaccat cagcccatac acgatggatc
     1621 tgctgatcga cgcgggttac acgtggttcg gcaacggttt ggccgacgac attccgcatt
     1681 attgggtcac cgatttcgcc gccaggcggg cgatcctgac gttgccctat tactaccact
     1741 tcgatga
//
LOCUS       DASWQN010000792         5118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1527760, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000792 DASWQN010000000
VERSION     DASWQN010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527760"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1874
                     /gene="aroB"
                     /locus_tag="VGC82_13350"
     CDS             195..1874
                     /gene="aroB"
                     /locus_tag="VGC82_13350"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY0424301.1"
                     /translation="MTRNTALRETIPIPDTIAGRSIVLVGLMGAGKTSIGRRLAARLG
                     LPFRDADQEIELAAGCTIPELFARYGETAFRDGERRVIRRLLAGDPMVLAFGGGAFLD
                     PRTREATRAEAVSVWLRCTLPTLVRRVATRDNRPLLTGRDREETLRELMEIRYPIYAE
                     ADVIVDCGDEPPDHTTTSVMNALQEWKPARRLHVVLPSTSYDVVIGDNLLARAGALLA
                     PRLPQKRAVVVTDQIVAGLHLQTLMDGLAETAITASSIVVKGGEASKSMDTYLDVVDQ
                     LLEARVERRTAVIALGGGVVGDLAGFAAATTLRGLPFIQIPTTLLSQVDSSVGGKTGV
                     NTKRGKNLVGAFYQPRMVLADTGTLGTLPPRELKAGYAEIAKAGLIGDAAFFTWCERH
                     GQAVISGDREAQAEAIRRACAFKAAVVGDDEREEKPNDGRALLNLGHTFGHALEAEYG
                     YDGGLLHGEGVAIGLGLAFKLSVRLGLCPPADAERVIAHVGAVGLPADLSMLNRRFSA
                     ETLIGHMRRDKKMRDGALHFVLARGIGQAFTSADVPPDAVMDLLRDEGCGA"
     gene            2009..2722
                     /locus_tag="VGC82_13355"
     CDS             2009..2722
                     /locus_tag="VGC82_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0424302.1"
                     /translation="MEAAEYALMDEAEDRMWWYRALHARLCDRLSGTTGQILDAGCGT
                     GGFLAVLHRQRPDLIRFGVEWDPTAALRSRQKSGAMIANGSVNLLPFADDSFDAAVSA
                     DVLCHAAADPAACLAELRRVLRPKGHLVLNMPAYRWLLAAHDRRVHNVRRLTASATAT
                     LLRQAGFERVQTRYWNFLLLPMMIAQRKILRRGDAVSDVAPFPPWLDATLHGITEIER
                     RLPFPLPAGGSVLAIAEKP"
     gene            2719..3708
                     /locus_tag="VGC82_13360"
     CDS             2719..3708
                     /locus_tag="VGC82_13360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0424303.1"
                     /translation="MNDTSTPAHTAPARAMQARPGLSIVVPVYRGAATIGRLVDALSD
                     LAPEGGLEVVLVNDGSPDNSAEVCRDLLATARVPLVYVEHARNYGEHNAVMTGLRHAS
                     GDFVITMDDDLQNPPEEVVKLYDHARLGGWDVVYTRYAVKQHEGWRNLGSRFANAVAD
                     RLLDKPKGLYLSSFRCMSALVVQAVSCYRGPYPYVDGLIMQVTQRIDSIEVRHLPRQE
                     GRSNYTLVRLVHLWLNLATSFSLAPLRLAIYAGVGLSILGTIGAVATITEALMMHDTP
                     SGWASLMTVLLLVSGVQSMILGVLGEYVGRIFLSANGKPQGTVRSVLRNTHTA"
     gene            3705..4037
                     /locus_tag="VGC82_13365"
     CDS             3705..4037
                     /locus_tag="VGC82_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEY0424304.1"
                     /translation="MSAYWAVLAAAIVTSMAGQTLLKAGAGASSFVSQLFDPRTMIGL
                     CLYGGAALLYIVALRRIPMSVALPCTAISYVAAALIGHYGFAEPLGAMHVGALALICA
                     GVVLLALA"
     gene            4207..4797
                     /locus_tag="VGC82_13370"
     CDS             4207..4797
                     /locus_tag="VGC82_13370"
                     /inference="COORDINATES: protein motif:HMM:NF024170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEY0424305.1"
                     /translation="MMAIPDLVNLSTLLDEAKCFELVRQHRWPQGARCPGCDSAAVVR
                     NGHDETQPHRQRYLCKACSRRFDDLTDTVLAGHHQPLRVWVLCLYLMGLNLSNRQIGE
                     ELGLNTSDVQTMTEHLRHGLVTRTPNVKLQGEVEIDEVYVVAGHKANRPKLQKRAAGT
                     ASQTKGRPRARHDGEGQAADPRPDPARRTGCFAHAT"
     gene            4924..>5118
                     /locus_tag="VGC82_13375"
     CDS             4924..>5118
                     /locus_tag="VGC82_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424306.1"
                     /translation="MAKIAQPGKAWAMEIWLAPFAAMFTQPTWLNAVALATGALRCLN
                     RRTVCSALRAVDGASDKGFSR"
BASE COUNT          849 a         1697 c         1688 g          884 t
ORIGIN      
        1 gcggtgtcgg cgggaacacc cccagcatca gcaccccgcc ggcgattgct accagcccgg
       61 ccagcacgat aaagaagaaa atgcgtatca tcgacgctcc tgaggtcggt tccgtggcgc
      121 cctggccgtt cgcgcatatc cgcgactcgc aggcaatcgc ctgatccggc accgatgtgc
      181 taacgtccgg aattatgaca cgcaacactg ctctgcggga gacgattccc atcccggaca
      241 ccatcgccgg acgcagcatc gtgctggtcg gcctgatggg ggccggcaaa acctcgatcg
      301 gccgccgcct cgccgcgcgc ctcggcctgc cgttccgcga cgccgaccag gaaatcgagc
      361 tcgccgccgg ctgcaccatt cccgagttgt tcgcccgcta cggcgaaacc gccttccggg
      421 acggcgaacg ccgggtcatc cgtcgcctcc tggccggcga cccgatggtc ctggccttcg
      481 gcggcggcgc cttcctggac cccaggaccc gcgaagccac ccgcgccgaa gcggtcagcg
      541 tctggctccg ctgcaccctg cccacccttg tgcgccgggt cgccacccgc gacaaccgcc
      601 ctttgctgac cggtcgcgac cgcgaggaaa cgctgcgcga attgatggaa atccgctatc
      661 ccatctatgc cgaagccgat gtcatcgtcg attgcggcga cgagcccccc gaccatacga
      721 cgaccagcgt catgaatgcg ctgcaggaat ggaagcccgc ccgccgcctg cacgtcgtgc
      781 tgcccagcac cagctacgac gtggtcatcg gcgacaacct gctggcccgc gccggtgcgc
      841 tcctggcacc gagactgccg cagaaacgcg ccgtggtcgt caccgaccag atcgtcgccg
      901 gcctgcattt gcaaaccctg atggacggac ttgccgagac cgcgatcacc gccagttcca
      961 tcgtcgtcaa aggcggtgag gcgtccaaaa gcatggatac ctacctcgac gtcgtggacc
     1021 agttgctgga ggcacgcgtg gaacgccgca ccgcggtcat cgcgctgggc ggcggcgtgg
     1081 tcggcgacct tgcgggattc gccgccgcga cgacgttgcg cggcctgccc ttcatccaga
     1141 tccccacgac ccttctctcg caggtggaca gctcggttgg cggcaagacc ggagtgaaca
     1201 ccaagcgcgg caagaacctg gtcggcgctt tctaccagcc tcgcatggtc ctggccgaca
     1261 ccggaacgct gggcaccctg ccgccgcggg agcttaaggc gggatacgcg gaaatcgcca
     1321 aggccggcct gatcggcgac gccgcgttct tcacctggtg cgagcgccac ggccaagccg
     1381 tcatctccgg cgaccgcgaa gcccaggccg aagccatcag gcgcgcctgc gccttcaagg
     1441 cggccgttgt cggcgacgac gaacgcgagg aaaagcccaa cgacggccga gcgctgctga
     1501 atttggggca cacgttcggc catgcgctgg aggcagagta cggctacgac ggcggcctgc
     1561 tgcacggcga gggcgtcgcg atcggcctcg gcctcgcttt caagctctcg gtccggcttg
     1621 gtctgtgccc gccggcggac gctgaacggg tgatcgccca tgtcggcgcc gtcggattgc
     1681 cggcggacct gtcgatgctg aaccgccgct tctcagcgga aacgctgatc ggccacatgc
     1741 ggcgcgataa gaagatgcgc gacggcgcct tgcatttcgt gctggcgcgg gggatcggcc
     1801 aagcgttcac cagcgccgac gtcccgcccg atgctgtgat ggacctgctg cgggacgaag
     1861 ggtgcggcgc gtagtggacc tttacccgac cgtcgccggc gggaccggcg ctgggttcgc
     1921 tagctggacc gtcgccggct gcaccgtggc tggctggacc gtggctggct ggaccgtcgc
     1981 gggctgggct gcctgataac tgtatcagat ggaagccgcc gaatacgcac tgatggacga
     2041 agccgaggac cgcatgtggt ggtaccgtgc cctccacgcc cgcctctgcg accgcctgtc
     2101 ggggacgacc gggcagatcc tggacgccgg ctgcggcacc ggcggctttc tggcggtctt
     2161 gcaccggcag cgccccgacc tgatccgctt cggcgtcgag tgggatccca ccgccgcgct
     2221 gcggtcacgc cagaaatccg gggccatgat cgcgaacggc agcgtcaacc tcctgccgtt
     2281 tgccgacgac agcttcgatg ccgccgtctc ggccgatgtg ctgtgccacg cggcggcgga
     2341 cccggcggcg tgtctggccg aactgaggcg cgtgctgcgt ccgaaaggac accttgtgct
     2401 gaacatgccg gcctatcgat ggctgctggc cgctcacgat cgccgggttc acaacgtccg
     2461 gcgtctgacg gcctccgcga ccgcgaccct gctgcgtcag gccgggttcg aaagggtgca
     2521 aacccgctac tggaatttcc tgctgctgcc gatgatgata gcgcagcgga aaatcctccg
     2581 tcgtggcgac gctgtgtcgg acgtcgcccc atttccacca tggcttgatg ccaccttgca
     2641 tggaataacc gagatcgaac gccggcttcc attcccgctt cccgccggcg ggtcggtgct
     2701 ggccatagca gagaaaccat gaacgacaca tccaccccag cccacaccgc gcccgcgcgg
     2761 gccatgcaag cccgccccgg attgtccatc gtcgtcccgg tttatcgcgg cgccgccacc
     2821 atcgggcggc tggtggacgc gctgtccgac cttgcccctg agggcgggct ggaggtcgtc
     2881 ctggtcaatg acggcagccc cgacaactcc gccgaagtgt gccgcgacct gctggcgacg
     2941 gcgcgtgtgc cgctggtcta tgtcgagcac gcccgcaact acggcgagca caacgcggtc
     3001 atgaccggcc tgcgccatgc cagcggcgat ttcgtcatca ccatggacga cgacctgcag
     3061 aatccgccgg aggaagtggt gaagctttac gaccacgccc gcctcggcgg ctgggacgtg
     3121 gtgtacaccc gctacgccgt gaaacagcat gaaggctggc gcaacctcgg cagccgcttc
     3181 gccaacgccg tcgccgaccg gctgctggac aagccaaagg ggctgtatct gtcgtccttc
     3241 cgctgcatgt cggccttggt ggtgcaggcg gtatcctgct accgcggccc ctacccctat
     3301 gtggacggcc tgatcatgca ggtcacccag cggatcgaca gcatcgaggt gcggcatttg
     3361 ccgcggcaag agggacgctc gaactacacc ttggtccgcc tcgtccattt atggctgaac
     3421 cttgccacca gcttctcgct cgcgccgctg cgcctggcga tctatgccgg cgtcgggctg
     3481 tcgatcctgg gcacgatcgg cgcggtcgcg acgatcaccg aggccctgat gatgcacgac
     3541 acgccatccg gctgggcatc gttgatgacg gtgctgctgc tggtgtccgg cgtgcagtcg
     3601 atgatcctgg gcgtactggg cgagtatgtc ggacgcatct tcctgtccgc caacggcaag
     3661 ccacagggta ccgtgcgaag cgtccttcgc aacacgcata cggcatgagc gcctattggg
     3721 cggtgctggc ggcggcgatc gtgaccagca tggcggggca aacgctgctg aaagccgggg
     3781 ccggggcatc cagcttcgtg tcccagttgt tcgacccgcg caccatgatc gggctttgcc
     3841 tgtatggcgg ggctgcgctg ctgtatatcg tggcgctgcg tcgcataccg atgtcggtcg
     3901 ccctgccgtg caccgcgatc tcatacgtgg cagcggcact catcgggcat tatggtttcg
     3961 ccgaaccgct gggtgccatg catgtcgggg cgctcgcgct gatctgcgcc ggggtcgtgc
     4021 tgctggcgtt ggcctgagcc accgtgaaat cccgccctac cgcccgccgg acaccgctgg
     4081 aaacgctccg taacccgagt ggctcttgtc gggtttcggg gtgatgggtc tggaaggtag
     4141 cgcaagctga aggtctctga tttgatccgg tgaagatgcc ttctggtttg ttcgaggagc
     4201 tgcgcgatga tggcgatacc cgatctggtc aatctctcga cactgctgga tgaggcgaag
     4261 tgtttcgagt tggtgcgcca gcatcgctgg ccgcaggggg cgcgctgtcc cggttgcgac
     4321 agtgcggcgg tcgtccgcaa cggccacgac gagacccagc cgcatcggca acgctatctt
     4381 tgcaaggcgt gcagcagacg tttcgacgat ctaaccgaca cggtgctggc cgggcatcac
     4441 cagccgttac gggtctgggt gctgtgtctc tatctgatgg ggctgaacct ttccaatcgg
     4501 cagatcggag aggaacttgg cttgaataca tcggacgtgc agacgatgac cgagcatctg
     4561 cgccacggcc tggtgactcg gacgccgaac gtcaaactgc aaggcgaagt cgagatcgat
     4621 gaagtctatg tcgtggccgg acacaaagcc aaccggccga agttgcaaaa aagggcggcc
     4681 gggacggcgt cgcagactaa aggacgcccc cgggcgaggc acgatggaga aggacaagcc
     4741 gccgatcctc ggcctgatcc agcgcggcgg acaggttgct ttgcacatgc tacctaaact
     4801 ggactttggc gatttcccag accgcttggt agcgtcgacg tagtcagctg cttgtgcggg
     4861 gatttggaga ttctgtaggc cgtagcgaag tttcccgtgt gtttgtcgca tgggaaaccg
     4921 atcgtggcga agattgcgca gcccgggaag gcctgggcga tggagatctg gctggcaccg
     4981 ttcgcggcga tgttcacgca gccaacgtgg ctgaatgcgg tggcgctggc gacgggagca
     5041 ctgcggtgtc tgaaccgccg cacggtatgc tcggcgctgc gggcggtgga cggcgcctcg
     5101 gacaaggggt tcagccgg
//
LOCUS       DASWQN010000793         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_604950, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000793 DASWQN010000000
VERSION     DASWQN010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604950"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1483
                     /locus_tag="VGC82_13380"
     CDS             83..1483
                     /locus_tag="VGC82_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424307.1"
                     /translation="MLEHLEKQESLTTNQWKIFAAATIGDMLDFFDFYLIGFILAFIV
                     GGWHLTYGQSGAILLASGIGAPIGSLFWGWMADKVGRRTVMILTVLNFSLATGLMALT
                     PDQGWLFLSICRFFVGVGVTGLYTVDIAIVQEFVPAYKRGWITGLTTTLLPAGTLLGA
                     LAGTYLEPYIGWRGLFAVGLLPALLTLLIRAWVPESPHWLIGKGRLDEARRSLAWALQ
                     VDPGTIQLPTSVAPVERALWRELFKYPRSMAVACLTGISQTGSVGLSLWMTTLFVLVL
                     KVSPGDASFLIIWVGAVSIFGRLFCSWISDAMGRRPAVVLSCLIAATTMSLAGYLNQV
                     YIGPVSMFYVMILAQSFFGSGNYSIVGPYMAEVWPSKLRASGMGFGYGFGNLGKFIGP
                     AGLAVLAGSDNFVTPKATLAALIPAMNYFAFWYVVGAAAIWFIGFETKGRTIEEIDET
                     LNASAAARSSPKVAAQ"
     gene            1587..>2816
                     /locus_tag="VGC82_13385"
     CDS             1587..>2816
                     /locus_tag="VGC82_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424308.1"
                     /translation="MSFMDRHLPTLSDALVLPIVRGTADLTGFAYQGGDFENLNDRIV
                     QFLADNPAEVRATARQYDEAIALQLAFRRSQGLNLQDAVLEKCQMFRVGAGFHTDRPL
                     RLLALLGPGDLMVNTPLDFLTNHLNVRLDLLYLLPDRKLPDEIPDHDVAFFAISEADP
                     ATLDRLRALFAAWPRPVLNDPACLPTLERDTLAKSLASIPGICSPTAVAVTRAALEVH
                     LRSGQPIDGFESSTGPYPCLIRPAGSHAGAGLARVSSAMELAEYLRLSFGHDFFLTAF
                     EDYSGLDGFYRKSRVAFIDKKPFLCHMAISTNWMVHYLNAGMTECAGKRAQEADAMAN
                     FDRTFAHRHAAAFQALHERLGFDFYSIDCAETRDGRLLVFEADAAAIIHLMDPPDMFP
                     YKQPQMHRVFSAFDTMLR"
BASE COUNT          482 a          896 c          832 g          606 t
ORIGIN      
        1 gccgaccttg tttgactcgc cgctcatctg catcgtcgtg ccaaatcaac aataattcca
       61 aatcgacgac tgggagaggc tgatgttgga acatctggaa aagcaggagt cgcttacaac
      121 gaatcagtgg aagatcttcg ccgcggcgac gatcggcgac atgctcgatt tcttcgactt
      181 ttacctgatt ggcttcatcc tggcgtttat cgtcggcggc tggcacctga cctacggaca
      241 gtcgggagcg atcctcctgg catccggcat tggcgcgccg atcggttcgc tgttctgggg
      301 ctggatggct gacaaagtcg ggcggcggac agtcatgatc ctgaccgtgc tgaacttctc
      361 gctcgccacc ggcctgatgg cgcttacacc cgatcagggc tggcttttcc tgtcgatctg
      421 ccgtttcttc gttggcgtcg gcgtgaccgg cctctacacc gtcgatatcg cgatcgtgca
      481 ggaattcgtg cccgcctata agcgcggctg gatcaccggc ttgaccacga cactgctgcc
      541 ggcgggcacg ctgctgggtg cgctggccgg aacctacctg gagccgtata tcggctggcg
      601 cggcctcttt gccgtggggc tgctgccggc cctgttgaca ctgttgattc gcgcctgggt
      661 tccagaatcg ccgcattggc taataggcaa aggccggctc gacgaagcac gcaggtcgct
      721 tgcctgggca ttgcaagtcg atcccggcac gattcaattg ccgacatctg ttgccccggt
      781 tgaacgggcc ctctggcgcg agttgttcaa atacccgcgc agcatggcgg tagcctgcct
      841 gacgggcatc agccaaacag gcagcgtcgg actgagcctg tggatgacga cgctgttcgt
      901 gctggtgctc aaggtcagcc ctggcgatgc gtcgttcctg atcatctggg tcggagcggt
      961 atcgatcttc ggacggttgt tctgttcatg gatttcggat gcgatggggc gccggcccgc
     1021 cgtcgtcttg agttgcctga ttgcagcaac gacgatgtcg ctcgcggggt atctgaacca
     1081 ggtctatatt ggtcccgtct cgatgttcta cgtcatgata ttggcgcaga gcttctttgg
     1141 cagcggcaac tactcgatcg tcggcccgta catggccgag gtctggccgt cgaagctgcg
     1201 cgcgagcggg atgggctttg gctacggctt cggcaacctg ggcaagttca tcggtccggc
     1261 gggcctggcg gtgcttgcgg gttccgacaa cttcgtcacg ccgaaggcga cgctcgccgc
     1321 gctgatcccg gcgatgaact actttgcctt ctggtatgtc gtcggtgccg ccgcgatctg
     1381 gtttatcggc tttgagacca agggacggac catcgaggaa atcgatgaaa cgttgaacgc
     1441 gtcggcggca gcgcgatcgt cacccaaagt ggccgcgcaa tagaccgctc gccaagtcac
     1501 accgaacact ccggccggtg ttcggtttaa ccattggtta accattggat gccaccctgg
     1561 cggcgcaacc cgctcaggaa cccgccatgt cgttcatgga cagacacctg cccacactca
     1621 gcgatgcgct ggtgctaccc atcgttcggg gaaccgcgga tctgacgggt ttcgcctacc
     1681 agggcggcga ctttgagaac ctgaatgacc ggattgtgca attcctcgcc gacaatccgg
     1741 ccgaggttcg cgcgacggcg cggcaatacg acgaagcgat cgccttgcaa ctggccttcc
     1801 gtcgttcgca aggcctcaac ctccaggacg ctgtgctcga gaaatgccag atgttccgcg
     1861 tcggcgccgg cttccatacg gatcgcccgt tgcgcctgct ggcgctgctc ggaccgggcg
     1921 acctcatggt caacacgcca ctcgacttcc tgaccaacca cctgaatgtc cggctggacc
     1981 tgctctacct tctgccggac cgcaagctgc cggatgaaat tcccgaccac gacgtcgcct
     2041 tcttcgcgat aagcgaagcc gatcccgcga cccttgatcg cttgcgcgcc ctgttcgccg
     2101 catggccacg cccggtcctg aatgacccgg cttgcctgcc gacgctggaa cgcgacaccc
     2161 tggccaagtc gttggcaagc attcccggca tttgcagtcc aaccgccgtc gccgtcaccc
     2221 gcgccgcgct ggaggtccac cttcggtccg gccagccgat cgacggtttc gagagttcga
     2281 ccgggcctta tccctgcctg atccggcctg ccgggtccca cgccggcgcc ggtctcgcac
     2341 gcgtttcatc ggcgatggaa ctggcagaat atctacgcct gtccttcggc catgattttt
     2401 ttctgaccgc attcgaggac tacagcggcc tcgatggctt ttaccgcaag tcccgcgtcg
     2461 ccttcatcga caagaaaccg ttcctttgcc atatggcgat ctcgaccaat tggatggtgc
     2521 actatttgaa tgccggcatg accgaatgcg ccgggaagcg ggcgcaggaa gccgatgcca
     2581 tggccaactt cgatcgaacc ttcgcgcacc gccacgccgc ggcattccag gccctgcacg
     2641 aacgtctcgg cttcgatttc tattcgatcg actgcgccga aacccgagac gggcgcctgc
     2701 tggtgttcga ggctgatgcc gccgccatca tccatctgat ggatccgccg gacatgtttc
     2761 cctataaaca gccgcagatg caccgtgttt tctccgcctt cgatacgatg ttgcgt
//
LOCUS       DASWQN010000794         2835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_209183, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000794 DASWQN010000000
VERSION     DASWQN010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209183"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VGC82_13390"
     CDS             <1..666
                     /locus_tag="VGC82_13390"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY0424309.1"
                     /translation="DRLLSGASDAARKVEIHEVAQDKGIMTMRLIEGGLFIEAGKTVK
                     FEPGGRHLMFIGLAAPFREGEQVSVSLVFEQAGKITVPLVVRGIGARAPDPLTKVELQ
                     RPTPLAAVDDPDEAFFTHFCDPKAMANITVSPGRAGAVEIAIQLEDGDERPLTTAHGV
                     SVTLANPDKGIAPVTAIAEHVGDDKWVVRMDAPQPGRWSLDLGIKLSATDGVNIEAPI
                     LIR"
     gene            complement(683..1858)
                     /locus_tag="VGC82_13395"
     CDS             complement(683..1858)
                     /locus_tag="VGC82_13395"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEY0424310.1"
                     /translation="MSVSRLRDYPGPALFSYGFRPFFFFGSVYAGAIILVWLPAFYGQ
                     LNLSTAFVPRDWHVHEMLFGYISAVVAGFLLTAVPNWTGRLPLQGRPLILLFSTWVAG
                     RIAVSSSAWIGWGLAASIDSVFLILLAASAAREIIAGKKWGNLMIVAIMCLLAAGNLA
                     FHLEAHFAGLAEYTTRGGIAVVISLVALIGGRIIPSFTRNWLAKRAPGQMPAPFGRFD
                     AATVGLSALVLLLWVIRPLDRTTGGLLLLCGGMHLIRLVRWSGYRTSADRLVLILHIG
                     YAFIPVGFILTALSAFDLVAPGAGIHAWTGGAIGTMTLAVMSRATLGHAGQRLEASPA
                     TQLIYAAVIIAALARVCAVLETDHAGLLLVVAGVAWAAAFLGFAAVYSAAFWSPRRL"
     gene            2246..>2835
                     /locus_tag="VGC82_13400"
     CDS             2246..>2835
                     /locus_tag="VGC82_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0424311.1"
                     /translation="MLSQSLNTSITSNKVAPVAPSPDQFWALTGHAGLLASDFSYRKD
                     EEIYGEDEPAEYVYQIVRGAVRTYKLLSDGRRQIGAFHLPGDVFGLQAGMTHRLAAEA
                     IIDTTVRLVKRHSLEQAAGSDVRVARRLWTMTASDLRHAEDHMLLLGRKSAMERVANF
                     LLEMDRRLAVAGMMALPMCRRDIGDYLGLSLETVSRA"
BASE COUNT          636 a          871 c          855 g          473 t
ORIGIN      
        1 gatcgtctgt tgtcgggcgc aagcgatgcc gccaggaaag tcgaaattca tgaggtggcc
       61 caagacaagg gcatcatgac catgcgtctt atcgaagggg gtctgttcat cgaagccggc
      121 aagacggtga agttcgagcc cggcggccgg cacctgatgt tcatcggact tgccgccccg
      181 ttcagggaag gtgagcaggt atcagtctcc ctggtgtttg aacaggccgg caagatcacg
      241 gtgccgcttg ttgtccgggg gattggagcg cgcgcgccgg atcccctgac caaagtggag
      301 ttacagaggc ccacgccact agcggccgtc gatgatcccg acgaggcctt cttcacgcat
      361 ttctgcgacc cgaaggcgat ggccaatatc acggtgtccc caggccgcgc cggtgcggtc
      421 gagatcgcaa tccagctcga ggatggtgac gagcgaccgc tcaccacggc ccacggcgta
      481 tcggtgacgc tcgccaatcc ggacaaaggc atcgcgccgg taaccgcgat cgccgaacat
      541 gtcggcgacg acaaatgggt ggtgcggatg gacgcgccgc agccaggacg ctggtcgctc
      601 gatctcggca tcaagttatc ggccaccgac ggggtcaaca ttgaggcccc gatcctcatc
      661 agataaatct caagaataaa tctcagagcc ggcgtggcga ccagaacgcc gccgagtaga
      721 ccgcggcaaa tccaagaaac gccgcagccc aagcgacacc ggcaaccacc agcaagagac
      781 cggcgtggtc cgtttccagc acggcgcaga cgcgcgcgag ggctgcaata atcactgcgg
      841 cgtagattag ctgtgtcgca ggcgacgcct cgagccgctg cccggcatga ccgagcgtgg
      901 cccggctcat cacagcgagc gtcatggtgc cgatcgcccc acccgtccat gcgtggattc
      961 cggcaccggg cgccacgaga tcgaaggcgc tgagcgccgt gaggatgaag cccaccggaa
     1021 tgaacgcata tccgatatgc aggatcagca ccagccggtc ggctgatgtc ctgtatccgc
     1081 tccagcgcac gaggcggatg aggtgcatgc cgccgcataa cagcaacaac ccgccggtag
     1141 tccggtccag cggtcggatc acccagagca agagaacgag cgcgctcaag ccaacggtcg
     1201 cagcatcgaa acggccgaac ggcgccggca tctggcccgg cgcacgtttt gccagccagt
     1261 ttcgcgtaaa actcgggatg atccggcctc cgatcagcgc aacgaggctg atcaccacgg
     1321 cgatgccgcc acgggtggtg tactcagcca agccagcgaa atgagcctcc agatgaaacg
     1381 ccagattgcc ggcagcgagc agacacatga tggcgacaat catgagattg ccccacttct
     1441 tgcccgcaat gatctcgcgc gcggcagaag ccgcaagcag gatgagaaag acggaatcga
     1501 tggaagcggc cagaccccag ccaatccagg ccgagctact gacggcaatt cggccggcaa
     1561 cccatgtgga gaacagcaga atcagcggcc tgccctgtag cggcagtctg cccgtccagt
     1621 tcggcaccgc cgtaagcagg aaacctgcga caacagctga tatatatccg aatagcatct
     1681 catggacatg ccagtcccgg ggcacgaacg ccgtactgag gttcagctgg ccgtaaaacg
     1741 ccgggagcca caccaggatt atggcgccgg catagacgga tccaaaaaag aagaacggcc
     1801 gaaaaccata cgaaaacaga gcgggaccgg gataatctct caagcgcgac actgacatga
     1861 ttaggacctc acttgggctg ttcttggcta gatcctagcc agcgtaacgc gcggcttctc
     1921 gtggaaccgc aaacagcttt gcggttcgct ccggatcgta tgcctctttc ggtgatcttg
     1981 tttgcgcaag cgcaaagaca acttccttgc gccgacatat tctttaacaa gcggttcagc
     2041 aaaatgggag atagacgttc cgaatccccg gcgcgccgaa gcagccgttc cttccggaga
     2101 gctgggtgaa agccagtcgc cctccggtaa accgcggggc gcctccggta aactacgtag
     2161 ggggttcccc ttaaggtatg aagcgaaatt tccagatcag attggctcgc gtaccaattg
     2221 gccatccgcc gcagaggaga tggccatgct cagccaatcg ctcaatactt cgattacaag
     2281 caacaaagtc gctccggttg ccccctcccc tgaccagttc tgggccctga cgggacacgc
     2341 ggggctcctg gcgagcgatt tttcctaccg caaggatgaa gaaatctacg gcgaggatga
     2401 gccagcggag tatgtctacc aaatcgtccg aggcgccgtg cggacttata agctgctctc
     2461 cgacggccgc cgccagatcg gcgcctttca tctgccaggc gacgtcttcg gactgcaagc
     2521 gggaatgact caccggctcg ccgcggaagc cattatcgat accaccgtgc ggctggtgaa
     2581 acgccacagc ctcgagcagg ccgcgggaag cgatgttcgg gttgcacgta ggctctggac
     2641 catgacggcc agcgatcttc gccatgcgga agaccacatg ctgcttctgg ggcgaaagag
     2701 cgccatggaa cgtgtcgcaa atttcctcct tgagatggac cgccgtctcg cagttgctgg
     2761 catgatggcg ctgcccatgt gccgtcgcga tatcggtgac tacctcgggc tatcgctgga
     2821 aacggtctca agggc
//
LOCUS       DASWQN010000795         2962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_341879, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000795 DASWQN010000000
VERSION     DASWQN010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341879"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..400)
                     /locus_tag="VGC82_13405"
     CDS             complement(2..400)
                     /locus_tag="VGC82_13405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY0424312.1"
                     /translation="MKGFTLLEVIIALVIAGIAATVLFEATGSGLHATQTAGMYDQAI
                     VRAKSRLAGATHGTKLSAGDWRGDDGGGFHWRLLVAPVASASVRQAGVVGPRAAASVP
                     VVLYAVSVWVSWNDGNTARDVRLDTEQVGG"
     gene            complement(397..1620)
                     /locus_tag="VGC82_13410"
     CDS             complement(397..1620)
                     /locus_tag="VGC82_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0424313.1"
                     /translation="MTIFRYTAIGAGGERLAGVMDAATEAEVIARLQRQGTMPVHAEP
                     ADRASRWAGLLQIDLGAGRGLRKQDVADLIRELATMLTAGQDLDRALRYMQETAPSRV
                     MPTVTGLRDAVRDGSPLSAAMARYPNSFPAMHVGLVRAGEAGGNLGPTLSRIADLLDR
                     QRALASTVTSALIYPALLFVAMIGAVMLLLTQVLPQFVPMFEQSGTALPGSTQFLIAA
                     GNFIQEDGLFLLLAIALLIVAARVALRQGRVRLGVDRLLLRLPVIGTLAREVLAARFT
                     RVLGTLLQNGVALIPALAIVRDAVGNWAARSAVDQASVTARGGGSLTRELERAEVFPP
                     RTIHLLRLGEETAQLAAMALRAADIHEEKTRLATQRLTALLVPAMTILMGLAVGGIVA
                     SLMTAMLSLNNLASG"
     gene            complement(1697..>2962)
                     /locus_tag="VGC82_13415"
     CDS             complement(1697..>2962)
                     /locus_tag="VGC82_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY0424314.1"
                     /translation="NRLYPEDTQARPGAETDAPLEEDAERLKDLASEAPVIRLVNLLI
                     TRAVETQASDIHIEPFEDRLRVRYRYDGVLHEAEPPPRHLAAAITSRIKIMARLDIAE
                     RRMPQDGRIKMAVRGQDVDFRVSTIPSLHGETVVLRILDRSAVVFDYVKLGLSPSVVH
                     KLGAALDLPNGIVLVTGPTGSGKTTTLYTGLLALNAVTRKIVTVEDPIEYQLQGINQI
                     QVRSQIGLSFASLLRSILRQDPDVIMVGEIRDGETAQIAVQAALTGHLVLSTLHTNSA
                     AAAVTRLRDMGVEDYLLTAVLRGIMAQRLVRRLCPDCRRREAAAPELIDRFDLDRRTA
                     DRPILLSRAVGCPACRQTGYRGRTAIAEFLRLGPEIERLIFARADHATIERAAVAEGM
                     EVMFDAGLAAALAGETTIEELTRSIRADA"
BASE COUNT          505 a         1023 c          963 g          471 t
ORIGIN      
        1 ttcatccacc gacctgttcg gtgtccagcc gcacatcgcg tgcggtattg ccgtcgttcc
       61 agctcaccca gacgctgacg gcatacaaca ccaccggcac ggacgccgcc gcgcggggcc
      121 ccacgacccc ggcctggcga accgaggccg atgcgacggg ggccacaagc aggcgccagt
      181 gaaagccgcc gccgtcgtcg ccgcgccagt ccccggccga cagcttggtg ccgtgggtcg
      241 ctccggccag gcgcgatttc gcccgcacga tggcctgatc gtacatcccc gcggtctggg
      301 tcgcatgcag gccgctcccg gtggcctcga acagcaccgt agcggcgatg ccggcgatca
      361 ccagcgcgat gatcacctcc agcagggtaa agcccttcat cccgacgcca ggttattgag
      421 gctgagcatc gccgtcatca gcgaggcgac gatcccgccg accgcgaggc ccatcaggat
      481 ggtcatcgcc ggcaccagca aggcggtcag ccgctgcgtc gccagacggg tcttctcctc
      541 gtggatgtcc gccgcacgca gcgccatggc ggcaagctgc gcggtttcct cgcccagccg
      601 cagcagatgg atggtgcgcg gcgggaacac ctcggcgcgt tccagttccc gcgtcagcga
      661 accgccgccg cgtgcggtca cgctggcctg gtccaccgcc gaccgtgccg cccaattgcc
      721 cacggcatcg cgcacaatgg ccagggccgg aataagtgca actccgttct gcagcaaagt
      781 gccgagaacc cgggtaaacc gcgccgccag cacttccctg gccagggtgc cgatcaccgg
      841 cagacgcagc aggaggcggt ctacgccgag gcgcacgcgc ccctgccgca gcgccacccg
      901 agcggcgacg atgagcagcg cgatcgccag cagcaggaac agcccgtctt cctggatgaa
      961 atttcccgcc gcgatcagga actgggtcga tcccggcagc gcggtgccgc tttgttcgaa
     1021 catcggtacg aactgcggca gcacctgggt cagcagcaac atgaccgcgc cgatcatcgc
     1081 cacgaaaagc agggccgggt agatcagcgc cgaggtcact gtggacgcca gcgcccgctg
     1141 ccggtccagc aggtcggcga tgcgcgacaa ggtcgggcca agattgccgc cggcctctcc
     1201 cgcacgcacc aacccgacat gcatcgctgg gaaactgttc ggataccgcg ccatagcggc
     1261 cgatagcgga ctgccgtcgc gcacggcatc gcgcaggccg gtgaccgtgg gcatcacccg
     1321 cgaaggcgct gtctcctgca tgtatcgcag cgcccgatcc aggtcctggc cggcggtcag
     1381 catcgtcgcc agttcgcgga tcaggtcggc gacgtcctgt tttctgagcc cccggccggc
     1441 gcccagatcg atctgcagca accccgccca gcgcgaggct cggtcggccg gttcggcgtg
     1501 cactggcatc gtgccctggc gctgcagcct ggcgatgacc tccgcctcgg tggccgcgtc
     1561 catgacgccc gcgaggcgtt cgccgccggc gccgatcgcg gtataacgaa atattgtcat
     1621 gtgtagcgaa acatcgtcat gtgtagcgaa acatcgtcat gtgaagcgaa acattgtcgt
     1681 atgcgtcgaa gcatggtcat gcgtctgccc gaatgctgcg ggtgagttcc tcgatcgtgg
     1741 tttcaccggc cagagcggcg gcgaggccgg catcgaacat cacctccata ccctcggcga
     1801 cggcggcccg ttcgatcgtg gcgtggtcag cccgcgcgaa gatcagccgt tcgatttccg
     1861 gccccagccg cagaaactcg gcaatcgcgg ttcgcccgcg atatccggtt tgccgacagg
     1921 ctgggcagcc gaccgctcgg gacaacagga ttggccggtc ggcggtgcgc cggtcgagat
     1981 cgaaccggtc gatcagttcc ggggctgccg cttcgcgccg ccggcagtcg ggacagagcc
     2041 gccgcaccag gcgttgcgcc atgatgccgc gcagtacggc tgtaagaagg taatcctcca
     2101 cccccatgtc gcgcagccgg gtcactgcgg cggcggcgga gttggtatgc agggtagaga
     2161 gcaccaggtg cccggtcagg gcagcctgga ccgcgatctg cgcggtctcg ccgtcgcgga
     2221 tttcaccgac catgatgacg tcggggtcct gtcgcaggat agaccgcagc aggctggcga
     2281 aactcaggcc gatctgcgag cgcacctgga tctggttgat gccttgaagc tggtattcga
     2341 tcgggtcctc gacggtgaca atcttgcggg tgaccgcatt cagcgccagc agcccggtgt
     2401 acagcgtcgt ggttttgccg ctgccggtcg gcccggtcac cagcacgatg ccgttcggca
     2461 gatccagcgc cgcccccaat ttatggacca ccgaagggga caaaccgagc ttgacgtagt
     2521 cgaaaaccac ggccgaacgg tcaaggatgc gcagcacgac cgtttccccg tgcagcgatg
     2581 gaatggtgga gacacggaaa tccacgtcct gaccgcgcac cgccatcttg atccgcccat
     2641 cctgcggcat ccgccgttcc gcgatgtcga gacgagccat gatcttgata cgggacgtga
     2701 tcgcagccgc gaggtgccgc ggcggcggct ccgcttcgtg cagcacgcca tcgtagcgat
     2761 agcggacgcg cagccggtct tcgaacggtt cgatgtggat gtccgaagcc tgcgtctcca
     2821 ccgcccgggt gatcagcagg ttcaccagcc ggatcaccgg cgcctcgctg gccaggtcct
     2881 tcagccgttc ggcgtcctct tccagtggcg catcggtctc ggcgccaggg cgggcctggg
     2941 tatcttcggg ataaagccga tt
//
LOCUS       DASWQN010000796         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_473239, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000796 DASWQN010000000
VERSION     DASWQN010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473239"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..2553
                     /locus_tag="VGC82_13420"
     CDS             190..2553
                     /locus_tag="VGC82_13420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0424315.1"
                     /translation="MPIDKPDAATGLTARVRFKASAASSGVAGTGGQPRPTTLGRQLG
                     WLVVAVVFPMLVFGLAGVALQYRAERANAARDALELARDLGSGLEADLKARGAALQTL
                     ALSPLLAAGDLAAFGQQLRQTTATQFPDSTILLWDAGGQVVSDTRSSLGTATPARDPP
                     PNLRQVFVTARPGISDVYTDVGLGRPAITLDVPVLREDGAVAYVLSITPGPSMVADQF
                     HSMPVREGWTVSIADRKGIFIARSKDSDHYVGQRAGAAFFARLPGTGASVAEIVNRKG
                     IPVLLTFRRSEPSGWTVVIGIPRKDLTDPAWHLTFLTLTAGCLLLLLGLALARIFARR
                     IIGPINALQMLSTQENRGTRAAAPFTTGLREVDEVGRTLLKSFTELRESRTELARVNA
                     ALESRVAEALAASDAAHERLAQGQKIQALGQLAGGIAHDFNNILQTMTGAATMIERRS
                     DGEERTKRFVRVMLEAASRGASITQRLLSFARRGTLSAEPIATAALLDGVREVLAHTL
                     GSPITVCTATKPDVPRILADRGQLETALINLGVNARDAMPDGGTLTLSARQERVIADS
                     PHPAGLAQGDYVRIAVTDTGTGMDPATLSRVTEPFFTTKPAGVGTGLGLSIVRGFAEQ
                     SGGALQIESAPGSGTIVTLWLPQAPDDALALGPRAATEVVAAGKSTGCVLLVDDDDLV
                     RETLVAQLEDLGFTTLAASSGRAAIALLEANEDVVALVCDLAMPDLNGIQTIRAARHR
                     RPELPCFLLTGYTGEHAALAGSDTFTVLRKPLSGGALAAHIEAVLAA"
     gene            2693..>3258
                     /locus_tag="VGC82_13425"
     CDS             2693..>3258
                     /locus_tag="VGC82_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424316.1"
                     /translation="MSLAMNEDKVLEKATMRRVSWRLMPFLMLAYLLCYIDRVNVGFA
                     SLQMNKAVGIDPKTYGLGAGIFFIGYFILEVPSNLALERFGARTWIARIMVTWGLVSA
                     AFALIGGPISFLILRFLLGAAEAGFFPGVILYLTYWYPAEYRAKIVGIFMVAIPVAGL
                     IGSPISGAILGMDGVLGLGGWQWIFLLEA"
BASE COUNT          547 a         1007 c         1052 g          652 t
ORIGIN      
        1 ccaacttctc gcgcgtttgc aattgcgttg gccggcacca acagtaccgg cagccaggag
       61 atactgatga caccatcgcc aaaggaccga agcgggccgg tgcgaatgac cggattgctg
      121 tctgctgggt tggaaaactg catcgcggaa ccccttcgga aagggacggc gccacggatc
      181 tagctacgga tgcctatcga caagcctgat gctgctaccg gattgaccgc ccgagtgcgg
      241 ttcaaggcaa gcgcagcctc atcaggcgtg gctggtacag gcgggcagcc gcgcccgacg
      301 acgttgggga gacagcttgg ctggctggtc gttgccgttg tcttcccgat gctggttttc
      361 ggccttgccg gggttgcgct gcaataccgg gcggaacgtg ccaacgcggc ccgcgatgcg
      421 ctggagctgg cgcgcgatct gggctccggt ctcgaagccg acctgaaggc acgcggtgcg
      481 gcgctgcaaa ctttggcttt gtcaccctta ctggccgcgg gcgatcttgc cgcgttcggt
      541 cagcagctcc ggcagacgac ggcgactcag tttccggact ctaccatcct gctgtgggac
      601 gcgggcggac aggtggtatc ggacactcgt tcctcattag gaacggcgac tccggcgcgc
      661 gatcctccgc ccaatctgcg ccaggtattc gttaccgcgc gccccggcat ctccgacgtt
      721 tacaccgacg tcggtttggg caggccagcc atcactctcg atgttccggt gctgcgcgag
      781 gacggcgctg tcgcttatgt gctatcgatc acgcccgggc cgagcatggt ggcggaccag
      841 tttcacagca tgcctgttcg cgaaggctgg acagtctcga tcgcggaccg aaagggaatc
      901 ttcatcgctc gcagcaagga tagtgatcac tatgtaggac aacgggccgg agccgcgttt
      961 ttcgctcgtt tgcccggcac cggcgccagc gttgccgaaa tcgtcaaccg gaagggtata
     1021 ccggtgctgt tgacgttccg ccggtcggaa ccgtcggggt ggacggtggt gatcggaatt
     1081 ccacgcaagg atctgaccga tccagcgtgg cacctgacat tcctgaccct gaccgctggc
     1141 tgcctgctgc tgctgcttgg tctggcgctg gctcggatct tcgcgcggcg gatcatcggc
     1201 ccgatcaacg cgctgcagat gctttccacc caggagaatc gtgggacacg tgcggctgct
     1261 cctttcacga ccgggctgcg cgaagtggac gaggtgggcc gcaccctttt gaaatctttt
     1321 acggaacttc gggaaagccg aaccgaactg gctcgggtga atgccgcgct tgaatccagg
     1381 gttgccgagg cacttgccgc cagcgacgca gcccacgagc gactcgccca agggcagaaa
     1441 atccaggcgc tcggccagct tgctgggggc atcgcgcatg atttcaacaa catcctgcaa
     1501 actatgaccg gcgcggcgac aatgatcgag cgacgttcgg acggtgagga aagaaccaaa
     1561 cgctttgttc gcgtcatgct ggaggcagcg tcccgcggag catcgatcac gcagcgcctg
     1621 ctgtcgtttg cccggcgtgg caccctgagc gcggagccga tcgcgacggc cgcactgctg
     1681 gacggggttc gagaggtatt ggcccacacg ctcggcagcc cgatcaccgt ttgcacggcc
     1741 accaagccgg atgtcccaag aattctcgcc gatcgcggcc aactcgaaac cgctttgatc
     1801 aatctcgggg tcaacgcgcg tgatgcgatg ccggacggcg gcacgctgac gctcagcgcg
     1861 aggcaggaac gggtcatagc ggacagtccc catcccgccg gcctcgcaca aggcgactac
     1921 gtgcgtatcg cggtcacgga caccggaacg ggaatggatc cggctacgct gtcgagggtg
     1981 accgaaccgt tcttcaccac caagccggcc ggagtcggca ccggcctggg tctttccatc
     2041 gtgcggggtt tcgcggagca atcgggcggc gcgctgcaga tcgaaagcgc tcccggatcg
     2101 ggaaccatcg tgacattgtg gttgccccag gcgcccgatg acgcgttggc gcttggtccg
     2161 cgggccgcca ccgaggtcgt ggctgctggc aagtccaccg gctgcgtgct tttggtagac
     2221 gacgatgatc tggttcgtga gaccctcgtg gcacagcttg aggatctggg cttcacgacc
     2281 ctggcggcga gtagcggccg ggcagcgatc gccctgttgg aagcgaacga ggacgtagtt
     2341 gcattggtat gcgacttggc tatgccggac ctgaacggca tccagacgat ccgagcggcg
     2401 cgccatcgcc ggccggaact gccttgtttt ctgctgactg gctataccgg cgagcatgcg
     2461 gcgcttgcag gatcggatac gttcaccgtg ctccgcaagc cgctttctgg cggagcgctg
     2521 gctgctcaca tcgaggctgt cctggcggcc tgagcggtca tacctctccg ccgcctgccg
     2581 gttgtccttg tgcaatcgtt cttgtgcaat tgtgcttaca ttgctgctgt ttcctctctg
     2641 gcgccggctg ccgatccctc ctacgctgcc gcgaaccttt ggttggaaac aaatgtccct
     2701 tgccatgaac gaggacaaag tcctcgaaaa agctacgatg cgccgcgtct cctggcgcct
     2761 gatgcctttc ctcatgctcg cctacctgct gtgctacatc gaccgggtca atgtcggctt
     2821 cgcttcgttg cagatgaaca aagcggttgg gatagacccc aagacctacg gcctgggtgc
     2881 cggcattttc ttcatcggct atttcatcct ggaagtaccg agcaacctgg cgctggagcg
     2941 gtttggcgcg cgaacgtgga tcgcgcggat catggtgacc tggggcctgg tctcggcggc
     3001 gttcgcgctg atcggcggac cgatttcgtt cctgatcctg cgcttcctgc ttggcgccgc
     3061 ggaagctggg tttttccccg gcgtgatcct gtatctgacc tactggtatc cggcggaata
     3121 ccgcgcgaaa atcgtcggca tcttcatggt ggccatcccg gtcgccggcc tgatcggctc
     3181 gcccatatcc ggcgcgatcc tcggcatgga cggggtgttg ggactgggcg gttggcagtg
     3241 gatcttcctg ctggaggc
//
LOCUS       DASWQN010000797         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2585572, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000797 DASWQN010000000
VERSION     DASWQN010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2585572"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..923)
                     /locus_tag="VGC82_13430"
     CDS             complement(150..923)
                     /locus_tag="VGC82_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY0424317.1"
                     /translation="MEQGQSEQVLSGPPSIVLDKVNKWYGTMHVLRDISLTVTEKERV
                     VVCGPSGSGKSTMIRCINRLETHQEGQIIVDGTELTDDLRALDTIRREVGMVFQSFNL
                     FPHLTILENCTLAPIWVRKMPKAEAEELAMSYLERVKIPEQARKYPGQLSGGQQQRVA
                     IARALCMKPKIMLFDEPTSALDPEMIKEVLDTMIGLAETGMTMVCVTHEMGFARQVAH
                     RVVFMDRGEIVESGPPRELFENPRTDRLKLFLSQILRGH"
     gene            complement(987..>1751)
                     /locus_tag="VGC82_13435"
     CDS             complement(987..>1751)
                     /locus_tag="VGC82_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0424318.1"
                     /translation="VVSAMRRFWRKELALIWVATLAAVGVLMWGGIFGLTEVTNDNWG
                     GLPLTLILATFGAAFSFPLAILVALGRRSTKLPAVKLLCVLYVELIRGVPLISVLFMA
                     SVMFPLFMPSGVNVDKLLRAQIAFILFSGAYLAEVVRGGLQALPKGQAEAADALGLSY
                     WKKTGFIILPQALRLVIPPLVNTFIGFFKDTSLVLIIGLFDLLTMGKVALSDPPWQSF
                     STEVYVGLAIIYIAFCFAMSRYSRSLERDLNRSRGR"
BASE COUNT          355 a          579 c          517 g          300 t
ORIGIN      
        1 cctccacgac cctccgtgtt gaagtgccgc ggccagccct ccgcaccaac ctcgcaggtc
       61 aacccccgat aaccaccccg accgcacggt acgtcggcgt gaatgcccgg cgcaagatcc
      121 agccagacat caaaccccgc ctccaggcct caatggcccc gtaaaatctg gcttaagaac
      181 agcttcaacc gatccgtccg gggattttcg aacaactcgc ggggcgggcc gctttccacg
      241 atctcgccgc ggtccatgaa caccacgcgg tgggcgacct ggcgggcaaa tcccatctcg
      301 tgtgtcacgc acaccatggt catgccggtc tcggccaggc cgatcatggt atccagcacc
      361 tccttgatca tttcgggatc caaggccgac gtaggctcgt cgaacagcat gatcttcggc
      421 ttcatgcaca gggccctggc gatagccacg cgctgctgct ggcccccgga caactgcccg
      481 ggatatttcc gagcctgttc ggggatcttg acgcgctcca ggtagctcat ggcgagttct
      541 tccgcctcgg ccttcggcat cttgcgcacc cagatcggcg ctaaagtaca gttctccagg
      601 atcgtcagat gcgggaacag gttgaacgac tggaacacca tcccgacctc acgccggatc
      661 gtgtccagcg cccgcagatc gtcggtcagt tcggtcccgt cgacgatgat ctggccctcc
      721 tggtgcgtct ccagccggtt gatgcagcgg atcatggtgg acttgccgga cccggacggg
      781 ccgcaaacca caacccgctc cttttcggtc accgtcaggg aaatgtcgcg cagcacgtgc
      841 atcgtgccat accacttgtt gaccttgtcc agcacgatcg acggcggccc cgaaaggacc
      901 tgttccgatt gaccctgttc catcttactg ttccgcatgg tttggctcat tgcaaaggat
      961 ccctgcacct tgatcggccc aactcgtcag cggccgcgag agcggttcag atcccgctcc
     1021 aggctgcggc tgtatctcga catggcgaaa cagaaggcga tgtagatgat cgcgaggccg
     1081 acatagactt cggtcgagaa gctctgccag ggcgggtcgc tcagcgcgac cttgcccatc
     1141 gtcagcaggt cgaacaggcc gatgatcagc accagcgacg tgtccttgaa gaatccaatg
     1201 aaagtgttga ccagcggtgg gatcaccaga cgcagcgcct ggggcaggat gatgaagccg
     1261 gtcttcttcc agtaactcag gccaagcgcg tcggcggcct cggcctgacc tttaggcagg
     1321 gcttgcaggc cgccgcggac gacctcggcc agataggcgc cggagaacag gatgaaggcg
     1381 atctgcgcgc gcagcagctt gtccacgttg accccggacg gcatgaacag cgggaacatg
     1441 acgctggcca tgaacagcac ggagatcagc ggcacgccgc ggatcaattc cacatacagc
     1501 acgcagagca atttcaccgc gggtagcttg gtagagcggc ggccgagggc gaccaggatg
     1561 gccagcggaa aagaaaacgc ggcaccgaac gtcgccagga tcagggtcag cggcagtccg
     1621 ccccagttgt cgttggtgac ctcggtgagg ccaaagatgc cgccccacat cagcacgccc
     1681 acggcggcca aggtcgccac ccagatcagc gccagttcct tgcgccagaa gcggcgcatg
     1741 gcggacacga c
//
LOCUS       DASWQN010000798         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_736373, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000798 DASWQN010000000
VERSION     DASWQN010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736373"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1140)
                     /locus_tag="VGC82_13440"
     CDS             complement(16..1140)
                     /locus_tag="VGC82_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY0424319.1"
                     /translation="MPATDVGTSEDLLAELSAWVRMETPTTDGAAVNRLMDVAHAELD
                     RAGATVTRIPGRHGYGDNLIARTVGEGKPVLVAGHLDTVWSHGTLDTMPYVVNGTKAH
                     GPGIYDMKAGSFLAFHAVRSILRQGVATPRPIVLLLTPDEEVGSPTSRWLIEQEAAQA
                     AAVLIPEPAGAGGACVTARKGVGRFVMRIEGRGSHAGGAFEDGASAVVELSHQILGLH
                     RMVDIDRGITLNAAPIWGGSRPNVISPDAGCEIDLRVNSAADGVLMEERLLGLTAKTS
                     GCRVIVEGGMNRPPYTESPAIAALYAHAQGIAQQVGLALPKQHRGGGSDGNFSAALGV
                     PTLDGLGCPGSGAHASHEHILWGHLAQRAALMAGLLETLR"
     gene            1254..1676
                     /locus_tag="VGC82_13445"
     CDS             1254..1676
                     /locus_tag="VGC82_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY0424320.1"
                     /translation="MSNLYFFEDLHVGQSATLSKTISEADILMYAAVSLDTNPVHFDE
                     EFAGKSRFGGRIAHGMLSAGLISALLGTRLPGPGTIYLRQSLAFKAPVRIGDTVKAVV
                     EITDLNPSRKHATLRTRCMVKENIVIEGEAVVLVPARG"
     gene            1676..>2019
                     /locus_tag="VGC82_13450"
     CDS             1676..>2019
                     /locus_tag="VGC82_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEY0424321.1"
                     /translation="MRIFTDWRDVPSDARGATVALGNFDGVHLGHASVIRAAHAARPD
                     APLAVLTFEPHPREVFRPDDPPFRLTLSPERADALAGLGVDLLYELRFNHDFSLMTAE
                     DFVRQVLHEGFGA"
BASE COUNT          351 a          729 c          568 g          371 t
ORIGIN      
        1 tccgtggttg acttctcatc tcaacgtttc cagcaacccc gccatcagcg ccgcccgctg
       61 cgccaaatgg ccccacagaa tatgctcatg gctcgcatgc gcacccgatc ccggacatcc
      121 cagcccgtcc agggtcggca cccctaacgc cgcagagaaa tttccgtccg aaccgccgcc
      181 gcgatgctgc ttcggcaacg ccaggccaac ctgctgcgca atgccctgag catgtgcata
      241 aagcgccgca atggccgggc tttcggtgta aggggggcgg ttcatgccac cctcgacgat
      301 caccctgcaa cccgacgtct tcgcggtcag gcccagcaac cgttcttcca tcaagacccc
      361 atcggcggcg gagttcactc gcaggtcaat ctcgcaccca gcatccggcg agatcacgtt
      421 gggccgtgac ccgccccaga tcggtgcggc attcagcgtg atcccacgat cgatatccac
      481 catccggtgc agccctagga tctggtgcga caactccacc acggcggacg cgccatcctc
      541 gaaagccccg ccagcgtgcg atccgcgtcc ctcgatccgc atgacgaaac gcccgacgcc
      601 cttcctggcg gtcacacagg cgccaccggc accggccggc tccgggatca gcaccgctgc
      661 cgcctgtgcc gcctcctgtt cgatcagcca ccgactggtt gggctgccga cttcctcgtc
      721 cggcgtcagc agcagcacga tcggccgggg tgtggcgacg ccctgccgca ggatcgaccg
      781 caccgcatgg aacgcgagga aactcccggc cttcatgtcg tagatgccgg gcccgtgcgc
      841 cttggtccca ttcaccacgt agggcatcgt gtccagcgtc ccgtgcgacc agaccgtatc
      901 cagatgaccc gcgaccagca ccggcttgcc ctcgccaacc gtccgagcga tcaggttgtc
      961 gccatacccg tgccgtccgg gaatccgcgt gaccgtggca cccgcccgat ccagttccgc
     1021 atgcgccacg tccatcaggc ggttcaccgc cgccccatcc gtcgtcggag tttccattct
     1081 gacccacgcc gacagctccg ccagcagatc ctccgaggtt ccaacatcag tcgccggcat
     1141 cgcgccctct catttccaca tcaagaccct gtgcgtctca tccgccagga cgtcgagttc
     1201 gcttgccctg ccgtccgttc gccgccaaga cataagaaaa ccgggatcgc ggaatgtcca
     1261 acctctattt ctttgaagac ttgcacgtcg ggcagagcgc cacgctatcc aagacgatca
     1321 gcgaggccga cattttgatg tatgcggctg tcagtctgga taccaaccct gtccatttcg
     1381 acgaagagtt cgctggcaaa tcccgcttcg gcgggcgcat cgcccacggc atgttgtccg
     1441 ccggcctgat ctctgccctg ctggggactc ggctgccggg acccggcacg atctacctgc
     1501 gccaatcgtt ggccttcaag gcaccggtcc ggattggcga caccgtcaag gccgtggtcg
     1561 agatcaccga tttgaatcca tcgcggaaac acgccacgct gcgcaccaga tgcatggtaa
     1621 aggagaatat cgtgatcgag ggtgaagcgg tcgttctggt gcctgcgcgc ggctgatgcg
     1681 gatttttacc gactggcggg acgtgccgtc cgatgcgcgc ggcgcgacgg tcgcacttgg
     1741 caattttgac ggcgtgcatc tgggccatgc cagcgtcata cgcgccgctc atgccgccag
     1801 gcccgacgcg ccactcgcgg tgctgacgtt cgagccgcat ccgcgcgagg ttttccgtcc
     1861 cgacgatcca ccattccgcc tgaccttgtc gcccgaacgc gccgatgcct tggctggcct
     1921 gggtgtcgat ctgctatatg aactgcggtt taaccacgat ttcagcctga tgaccgccga
     1981 ggacttcgtc cgccaagtgc tgcacgaggg ctttggtgc
//
LOCUS       DASWQN010000799         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1397775, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000799 DASWQN010000000
VERSION     DASWQN010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397775"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2040
                     /locus_tag="VGC82_13455"
     CDS             <1..2040
                     /locus_tag="VGC82_13455"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0424322.1"
                     /translation="DIVAVVIATSLTAARDAAEHVVVDFRPLPSVSHALDAVADGAAA
                     AWAEGPSNTPIDATLGDSAATAAAFAAAAHVVRFDTWIQRVAGVPMEPRSAIGVYDST
                     QAKYTLHAGAGGAVRPRNDLAAVLGVPETDVRMVMHDVGGNFGTRGAFNPEFALVVWA
                     SRRVGRPVKWTCDRAEAFLCDYQARDLAVTAELALDAEGTFLGLRCSNLSNLGAYLVG
                     FSPLQKGAAIVASIYHVPAVAVRARGAFTNTAPTRPYRSAGRPEVIYVMERLIDLAAR
                     QTGLDRITLRRRNLVPKTAMPYRNPFGLVYDSGAYHRVMETALRLGDWEGFTARQAEA
                     AGRGKCRGIGVANYVDTATGVPRERAEVTVKPEGVVEVVIGTGSQGQGHETSFAQLVS
                     EWLGVPHESVVLVTGDTDRVKWGGGAHSGRALRLGSIVMRNASTEIVEKAMRIASHVL
                     EANPSDIVFSGGQFVVAGTDRAIGIFAVAAAALARNDLPEDLAGPLGAVSDETIRDAS
                     FPYGCHVCEVEIDPETGSVEIISYTAVDDVGRAVNPMIVHGQVHGGIAQGVGQALWEQ
                     VVYDRTTGQMLTGSFMDYAMPRAGGLPSYVTELSEVPSTTHPLGIRPAGEGGTTPALA
                     VVVNAVVDALAGFGVTHVEMPTTPERIWRAIRAAQRHGRLEDASSAATGQIAQPL"
BASE COUNT          353 a          643 c          744 g          407 t
ORIGIN      
        1 gacatcgttg ccgtggttat tgcgaccagc ctcacagcgg cccgcgacgc ggcggagcat
       61 gtggtcgtgg attttcggcc gttgccttcg gtgtcccacg ctctcgatgc tgtggccgac
      121 ggcgcggcgg ccgcctgggc ggagggacca tcaaataccc cgattgatgc gacgctcggc
      181 gactcagccg ccaccgcggc ggcattcgct gcagcggcgc atgtggtccg gttcgacacc
      241 tggattcagc gggtagccgg tgtgccgatg gaaccccgct cggcgatcgg agtgtatgac
      301 tccacccagg ccaaatacac gctgcatgcc ggtgccggcg gagcggtgcg gccgcgcaac
      361 gacctcgctg cggtactggg cgtgccggag actgatgttc gcatggtcat gcacgatgtc
      421 ggcggcaatt tcggcactcg cggcgccttc aatccagaat tcgcgttggt ggtctgggct
      481 tcgcggcggg tcggccgccc ggtgaaatgg acctgcgacc gagcggaagc atttctgtgc
      541 gattaccagg cgcgggatct ggcggtgacc gccgaactcg ctttggatgc agaaggcacg
      601 ttcctgggcc tgcggtgctc caacctgagc aatctcggcg cctatctggt cgggttcagc
      661 ccgctacaga aaggtgctgc catcgtcgcc agcatctacc atgtgcccgc agtggcggtt
      721 cgtgcacgcg gcgccttcac caacacggcg ccgacccggc cttatcgcag cgccgggcgg
      781 ccggaggtga tatatgtgat ggagcggttg atcgacctgg cggcgcggca gaccgggctc
      841 gaccgcatca ccctccgccg tcgcaatttg gttccgaaaa ccgcgatgcc gtatcgaaat
      901 ccgttcggct tggtttacga cagcggtgcc tatcatcggg tcatggagac cgcgctgcgg
      961 cttggcgact gggagggctt caccgcccgc caagccgagg cggccgggcg cggcaaatgc
     1021 cgtggcatcg gtgtcgccaa ctatgtcgac acggcgaccg gtgtgccgcg cgaacgggcc
     1081 gaggtcacgg tgaaacccga aggcgtggtc gaggtcgtta tcggcactgg ttcccaggga
     1141 caggggcatg agacgagttt cgcccaattg gtctcagaat ggctgggtgt cccgcatgaa
     1201 agtgtggtgc tggtgaccgg cgatacggac cgcgtgaagt ggggcggcgg cgcgcactcc
     1261 ggccgcgcgc tgcgattggg cagcattgtc atgcgtaacg cttcgacaga gatcgttgag
     1321 aaagccatgc ggattgccag ccatgtgctg gaggccaatc cctccgatat cgtgttcagc
     1381 ggcggacagt tcgttgtcgc cgggaccgat cgcgcgatcg gcatttttgc cgttgcggcg
     1441 gcggcactgg cccgaaacga tctgccggag gatctggcgg gtccgctggg cgccgtcagt
     1501 gacgaaacga tcagggacgc gagctttcct tatggctgcc acgtctgcga ggtcgagatc
     1561 gatccggaga cggggtcggt agagatcatt tcctacaccg cggtggatga tgtaggacgc
     1621 gcggtgaatc caatgatcgt gcatgggcaa gtacacggtg ggatcgcgca aggcgtcggc
     1681 caggcgctat gggaacaggt tgtttacgat cgaacgaccg ggcagatgct caccggctcc
     1741 ttcatggatt acgcgatgcc gcgcgcagga gggctgcctt cctatgtcac tgaacttagt
     1801 gaggtgccgt cgacgacgca cccgcttggc attcgtcccg ccggtgaggg tgggactacg
     1861 ccggcgctcg cggtggtggt aaatgcggtg gttgacgcgc ttgccggttt tggcgtcacc
     1921 catgttgaga tgccgacgac gccggagcgg atttggcggg cgatccgtgc cgcgcagcgc
     1981 catggtagac tggaggacgc gtcgtcagcg gccactggtc aaattgcgca gccgctttga
     2041 ccgccactgc gtttgttgct cacctttgct ggctccagcg cgatcaaccc cttgcctggc
     2101 agatacagtc ccatacgcgg tccaaggcac cattcggggg ccaccag
//
LOCUS       DASWQN010000800         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2057670, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000800 DASWQN010000000
VERSION     DASWQN010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2057670"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VGC82_13460"
     CDS             <1..825
                     /locus_tag="VGC82_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEY0424323.1"
                     /translation="RLNLGQAVQVLDPATDRDVFDKLIPEYQLLTGRRGTPPESAARR
                     VATRPSVAAAMLLHGGQADAAICGGTGAWWRQIQYILPIVPRRPDVSRVYALSCLILQ
                     TGVLFICDTFMVLDPNAEQITEMTLLAAEAVRGFGLIPKAALVSHSSFGASDSQSARK
                     MRQALSMIRARAPGLEIDGEMHADAALVQTIRDHAVPDSRLSGSANLLIMPTLDAANI
                     SFNVLKAAADGLPVGPILLGMSKPIHVVVPSVTARGIVNLSALAVVEAQEQAGATA"
     gene            828..1268
                     /locus_tag="VGC82_13465"
     CDS             828..1268
                     /locus_tag="VGC82_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY0424324.1"
                     /translation="MLWLLFGAVVLFLLMGGLRAFERASVTSVKSLFTWIAALGGLSL
                     ALLLILTGRGGIALGALTLFGPLLYQQWQAARGRRIGGSAPGRSSGPMTREEAYQVLG
                     LHQNATQSEIREAHRRLMRGAHPDAGGSDWLAARINQARDILLG"
     gene            1403..>2165
                     /locus_tag="VGC82_13470"
     CDS             1403..>2165
                     /locus_tag="VGC82_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010501497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEY0424325.1"
                     /translation="MIKKLRKAVLPVAGLGTRFLPATKATAKEMLPVVDKPLIQYAVE
                     EARAAGIEQFCMVTGRGKTALVEHFDVAYELEATLRERDKKDALASLSGAQSEPGSIT
                     TVRQQVPLGLGHAIWCARAFIGDDPFAVLLPDDLVLSDQPCLAQLADAYYETGGNIVA
                     VTEVPREQTNRYGILKIGKDDGRLVEVTGLVEKPSPEDAPSNLSIIGRYILMPEVIKY
                     LALMERGAGNEVQLTDGMARLIGEQPFHGLRYKGRR"
BASE COUNT          366 a          687 c          709 g          403 t
ORIGIN      
        1 cgcctgaacc tgggccaggc tgtgcaagtg ctggacccgg caaccgaccg ggacgtattc
       61 gacaagctta tcccggaata ccagcttctg acgggccgcc gcggcactcc ccccgaatct
      121 gccgctcggc gggtcgcgac ccggccatcg gtagcggcgg cgatgctgtt gcacggtggc
      181 caggcggatg ccgcgatctg cggcggcacc ggcgcctggt ggcggcaaat ccagtatatc
      241 ctgccaatcg tcccgcgccg tcccgatgtc agccgggtat acgcactgtc gtgccttatc
      301 ctgcagactg gtgtgctgtt tatctgcgat accttcatgg tgctcgatcc gaacgccgaa
      361 caaatcaccg agatgacgtt gctggcggcg gaagcggtgc gtggtttcgg gctgattccg
      421 aaggccgcgc tggtctcgca ctccagtttc ggcgccagcg acagccagtc cgcgcgcaag
      481 atgcgccagg cgctgtcgat gatccgagcg cgcgcccccg ggctggagat cgacggcgag
      541 atgcatgccg acgcggcgct ggtgcagacg attcgcgatc acgccgtgcc ggacagccgg
      601 ctgagcggca gtgccaacct gctgatcatg ccaacgctgg atgccgcgaa tatctcgttc
      661 aacgtgttga aggccgcggc ggacgggctg ccggtcgggc cgatcctgtt gggtatgtcc
      721 aaacccatcc atgtggtcgt ccccagcgtc accgcgcgcg gcatcgtcaa cctgagtgcc
      781 ctggcggtgg tggaggcgca ggagcaagcc ggcgcgaccg cttagccatg ctatggctcc
      841 tgttcggtgc cgtcgtcctg ttcctgctga tgggcggctt gcgcgccttc gagcgggctt
      901 cggtcacctc ggtgaaatcg ctgttcacct ggattgccgc gcttggcggc ctcagtctgg
      961 ctttgctgct gatcctgacc ggccgcggcg gtatcgctct tggcgcgctg actctgttcg
     1021 gaccgctgtt gtatcagcaa tggcaggcgg cccgcggccg gcggatcggc ggcagcgcgc
     1081 ccgggcgctc gtctggcccg atgacccgcg aggaagccta ccaggtgctg ggtctgcacc
     1141 agaatgctac ccagagcgaa atccgcgagg cgcaccgccg gctgatgcgc ggcgcccatc
     1201 ccgatgccgg cggatcggac tggcttgccg ccaggatcaa ccaggctcgc gacattctgc
     1261 ttgggtaatg caggcaaggc gccctatttc accaccaacg gtcggcttta acactcggac
     1321 atgggcagcg attctcttgc aggctgccat attttggccc gaagcgcgtg atgcctacag
     1381 gaaattttaa tggagtcgcc gcgtgatcaa aaaactgcgc aaagccgttc tccccgtcgc
     1441 tggcctgggc acccggtttt tgcccgccac taaagcgact gccaaggaaa tgcttcccgt
     1501 cgtcgataaa ccgctgatcc agtatgccgt cgaagaggcg cgcgcagctg gcatcgagca
     1561 gttctgcatg gtcaccggac gcggcaagac tgcgttggtg gagcacttcg acgtcgctta
     1621 cgaactggaa gcgactctgc gcgaacgtga caagaaggac gcgctggcgt cattgagcgg
     1681 ggcgcagagc gagccgggct ccatcaccac ggtgcgccag caggtgccgc tcgggctggg
     1741 ccacgcgatc tggtgtgccc gcgccttcat cggggacgat ccgttcgcgg tgctgctgcc
     1801 ggacgacctg gtgctgtccg accagccatg cctcgcgcag cttgcagatg cctactatga
     1861 aaccggcggc aacatcgtcg cggtcaccga agtgccgcgt gagcaaacca accgctatgg
     1921 cattctgaag atcggcaagg acgatggccg cctggtcgaa gtcaccgggt tggtcgagaa
     1981 gccctccccc gaggatgcgc cgtccaactt gtcgatcatc gggcgctaca tcctgatgcc
     2041 ggaagttatc aaatacctgg cgttgatgga gcgcggcgcc ggtaacgagg tgcagcttac
     2101 ggatggcatg gcaaggttga tcggcgagca gccgttccac ggtttgcgct acaaaggtcg
     2161 tcgtt
//
LOCUS       DASWQN010000801         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1398275, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000801 DASWQN010000000
VERSION     DASWQN010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398275"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VGC82_13475"
     CDS             <1..227
                     /locus_tag="VGC82_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEY0424326.1"
                     /translation="KMEQGEDMFHKRRSLFFKKMQIVRGCDAKRNVLVYMVYSDRLIE
                     GSPKNSTSSVPIMPWGAADSAVQKCGECIQ"
     gene            427..597
                     /locus_tag="VGC82_13480"
     CDS             427..597
                     /locus_tag="VGC82_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424327.1"
                     /translation="MRAFSFILAVALVVAAPSIAGSVDTSLPGVGTFAYSGPSVAASA
                     SPAIPSADRTRS"
     gene            complement(638..919)
                     /locus_tag="VGC82_13485"
     CDS             complement(638..919)
                     /locus_tag="VGC82_13485"
                     /inference="COORDINATES: protein motif:HMM:NF042781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6719 family protein"
                     /protein_id="HEY0424328.1"
                     /translation="MSQIATPIMSTSRAGPVLWLLLGGFALAALSGPAAAEQVSREQD
                     IVNLRLGQRILVDDGSCPPGQIKEVSGARMTADGILRTQKCIARSGTRK"
     gene            complement(916..1068)
                     /locus_tag="VGC82_13490"
     CDS             complement(916..1068)
                     /locus_tag="VGC82_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424329.1"
                     /translation="MSGNRNILFLIIGALIVAVGVLGYNLYQTKKEPEGVQINLGPNG
                     LKIQNK"
     gene            1153..>1577
                     /locus_tag="VGC82_13495"
     CDS             1153..>1577
                     /locus_tag="VGC82_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0424330.1"
                     /translation="MNATDTKRKILDAVDAGFDAQLAVTRDFVAIPSTRGAEGPCQDM
                     IGDLLRQRGYEVDDWHIEVDDLRDMRGFGPIEHDFSKARTVVGTYRPQSNVGKSLILQ
                     GHCDVVPAGPLEMWKTPPFSPVVKDGRMYGRGACDMKSGT"
BASE COUNT          294 a          501 c          485 g          297 t
ORIGIN      
        1 acaagatgga gcagggcgaa gacatgtttc acaaacgccg ctcgctgttc ttcaagaaaa
       61 tgcagatcgt gcgtggctgc gacgccaagc gcaatgtgct ggtctatatg gtctattcgg
      121 accggctgat cgaagggtcg ccgaagaatt caacctcgtc ggttccaatc atgccgtggg
      181 gcgctgcgga tagcgcggtc caaaaatgcg gcgaatgcat tcagtgaagg ccgctttacc
      241 ccggcttcgc aaggtgaggt agccggcgaa gccgcgcgac tgtgtcgtcc cgcccaggcg
      301 acgaaacaaa tcttccgggc aacgaaacca ttctccgctt tcgcgaaaat ccccggaaac
      361 gctgcgtggt taccaagctg accatggacc ggcagaaagt gccggcggcg aacccggagc
      421 agtcagatgc gggccttcag cttcatcctt gccgtagcct tggtggtggc tgccccctcc
      481 atcgccggct cggtcgatac cagcctgccg ggggtgggca ccttcgctta cagcgggccc
      541 tcggtcgcgg cctcggcttc cccggccatc ccgtccgcgg atcgcacccg cagctgaccg
      601 ggaccgctgg gaaagttgcc gaccgtcccc ggtccggtta tttccgggtc ccggatcgcg
      661 caatgcactt ttgggtgcgg aggatcccgt cggccgtcat cctcgcgccg gaaacttcct
      721 tgatttgccc gggcgggcac gatccgtcgt cgaccaggat gcgctgcccg agccgcaggt
      781 tgacgatgtc ctgttcgcgc gacacctgtt ccgccgccgc cggacccgac aacgcggcca
      841 gcgcgaaacc gcccagcaat agccacagaa caggcccggc gcgtgaggta ctcatgatcg
      901 gtgtggcgat ctgactcact tgttctgtat cttcaacccg ttcggaccga gattgatctg
      961 cacgccttcc ggctccttct tggtctggta aagattgtag cccagcacgc ccaccgccac
     1021 gatcagcgct ccgataatga gaaacagaat attgcggttg ccggacatcc gtggctccat
     1081 ggctcgcggg attttgcggg agtgtatggg cccgcttgcg attctgaaag cgacgacggg
     1141 acacgtgacg cgatgaatgc caccgacacc aagcgtaaaa tcctcgatgc ggtcgatgcc
     1201 ggtttcgacg cccaacttgc cgtcacccgc gatttcgtcg cgattccctc cacccgcggc
     1261 gccgagggcc cctgccagga catgatcggc gatctgctgc ggcagcgcgg ttatgaggtc
     1321 gatgactggc atatcgaggt cgacgatctc agggatatgc gcggcttcgg accgatcgag
     1381 catgatttct caaaggcgcg caccgtggtc ggaacctacc gtccgcaaag caatgtcggg
     1441 aagtcgctga tcctgcaggg gcattgcgac gtggtacccg ccggcccgct cgagatgtgg
     1501 aagacgccgc cattctcccc ggtggtcaag gacggcagga tgtatggccg cggcgcctgc
     1561 gacatgaagt ccggcac
//
LOCUS       DASWQN010000802        11739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1793902, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000802 DASWQN010000000
VERSION     DASWQN010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11739
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793902"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VGC82_13500"
     CDS             <1..1193
                     /locus_tag="VGC82_13500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0424331.1"
                     /translation="AADAPAASVTVEPIQVRNVAPASTYIGRVQAIQSVAIVARVQAF
                     VDKIDFQEGAMVKAGQLLFELQKAPYQAAVEAAQAGLQRAEATLRNAQLIYERDSRLG
                     RGAVVSQSQIDADLATRDADNADVLSARANLETAAINLSYTSITSPIDGRIGKAAYTV
                     GNLVGASASAGPLATVVQVDPIRVIFSVADRDIVSALQRTHQTSAKIASTLSLDLQLP
                     DGQRYPQHGEIEFIDNAVDPATGTVSVWGRFANPEGLLIPGGFVSVDVQRATPEERPL
                     VPVQAVQDDKAGHFVLLVGPDNKVSQQTISIDRQIGQDWIVTAGLKGGENVIIEGLQK
                     VQPGQVVSPVQAQTQNEQTGSSPNGSSNGASSHAASSNGVSSNGASSGNEQASVDTRP
                     GQGH"
     gene            1197..4343
                     /locus_tag="VGC82_13505"
     CDS             1197..4343
                     /locus_tag="VGC82_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEY0424332.1"
                     /translation="MLSSFFIDRPKFAIVIAIVITLAGLLALWVIPVAQYPNITPPQI
                     TVSANYPGADAETVANTIGIPIEEQVNGVQNALYMSSTSSSSGSYTLTVTFAIGSDPN
                     IDQVNVQNRVSLAQAQLPATVAQQGLTVLQQSTNFVIAVNLFSPNNKYDQTFISNYAN
                     VNLRYPLARLSGVGNAQILGNAQYAMRIWMDPVRMTSLNVSPSDVVNAISTQNQQSAA
                     GQIGAPPISGGQRQQLTIITQGRLASADQFRDIIVKTNANGGIVRIRDIADVELGAQT
                     YASTSRLNQFPSATLAIYQAPGANALELANAIQQQVKEIAKDFPPGLEYAVVYDSTRF
                     VRANISEILTTLGITLLLVIAVVYVFLQDWRATLIPTCAIPVSLIGVFAVLYMLGYSA
                     NTIDLFAIVLAITLVVDDAIVVVENVTRHLEEHPQQPPREATREAMAEIIGPVIASTL
                     VLVAVFAPVGFLPGISGQLFRQFAVTISVSVVISGVNALTLSPALCALMLRPPKQARF
                     RLFTGFNRALNWTRGVYGGTVRWLGRRLLVGGVALGGVLVAAYLLFAAVLAGFLPNED
                     QGYFFVNVGLPNGAALVQTEQVIDRIGQMVRSTPGVTDAIELSGFSIVNGTQQPNAGA
                     VIVILKPWDQRTAADMRAEAIIGRLQTQFNALPTATITAFNPPSIPGLGHTGGLDLQM
                     QGRTGQSYPDLAATARALIYAGNQDPSLSSVFTTFSASVPEVMVRVNTARAELLGVSP
                     NDIYTTLQAHLGSQFVNYFNLQSQVFQVIVQDASRFRAQVSDINQLYVRSASGAMVPM
                     NSLIDITTVQGSNAVTRYNLYPSVEINGSARGSASSGQAMQAMAAVAAQHLPPGYGYE
                     WTSLSYQQQQGGGAGGTVFLFALVFAYLFLVAQYESWSLPMSVILSVSVAALGALLAL
                     WVRGLALDVYGQVGLVLLIGLAAKNAILIVEFAKNRVDHGDEVREAAQAGATTRFRAV
                     LMTAIAFIIGVIPLVIATGAGAGARQSIGTTVFGGMVLATILGIVSVPVLFIMFEFLA
                     RWSGHLLQRREVRGRPAE"
     gene            complement(4717..5265)
                     /locus_tag="VGC82_13510"
     CDS             complement(4717..5265)
                     /locus_tag="VGC82_13510"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0424333.1"
                     /translation="MARVPYLDPSDLAPEDQDLLKRPISLFKALVNSPKAARAFSGLG
                     GYIRYGSKLDPRLRELAILQVGWLARSPYEWSHHVKLGHDFGVSDEDIQALIDEPAGK
                     PTSLGSLPRLVLRSAREMTNDGAMTEATFTALQAELGNELVVDLTLAIGFYNAVVRVL
                     ATLQIDVEPDYMPYLERFPLPA"
     gene            5428..5919
                     /locus_tag="VGC82_13515"
     CDS             5428..5919
                     /locus_tag="VGC82_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY0424334.1"
                     /translation="MRATVTPAGNLTMDGDTFRVALGYGGIRADKREGDGATPAGLLP
                     LRTVLYRPDRQPAPICAVSVQRLAPYDGWCDDPTHAAYNSLVRLPINARAEALWREDA
                     LYDIIGVLGWNDRPVEPARGSAIFLHVARPDFASTEGCVALSLETLLDVLSKGLTEIL
                     VTV"
     gene            complement(5909..6910)
                     /locus_tag="VGC82_13520"
     CDS             complement(5909..6910)
                     /locus_tag="VGC82_13520"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0424335.1"
                     /translation="MHRFAAIGLDHRHVYDLTAGLLAAGATCVGHDPETSDPRVLAGF
                     RKRFPDVPAVTRDRLFDDPSVDFIVLAAVPRDRAGLAIKAMQRGKDVMVDKPGVTTLD
                     QLASVEAIVRETGRIWSVALGRLTSPAVQAALAVARSGELGRLVSLTSLAPHRLNRAL
                     RPAWFFEQEAYGGIITDIGVHSIDQFLAFADATEATIAASTIGMFGTPPEGFEDFAEV
                     TMRSGTMTGTMRMDWFTPDGLPDWGDGRLFLVGTQGTLELRKNLDIEGRAGGDHLFVA
                     NRERTRYQDCSLLPVTYYSDFLADLRDRKGRTLDGGRVFSACRLALQCQGRAERFRP"
     gene            7005..7751
                     /locus_tag="VGC82_13525"
     CDS             7005..7751
                     /locus_tag="VGC82_13525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0424336.1"
                     /translation="MTDRVAIITGAGSGIGRATALNLLAGGWHVALVGRRPEALEETA
                     GLATGGKTLVVPTDVTEPAAVDALFAKVKSTYGRLDTLFNNAGGNVPTTNFGDFTFEM
                     WRKVCAINLDAMFLCANAAFRAMRDQEPQGGRIINNGSISAHNPRPGSVAYTSTKHAV
                     TGLTKSIALDGRQYNICSSQVDIGNAATPMTARSTNGQMQPYGQMAVEPRMDADHVGK
                     QVAFMANLPLESNVQFVTIMATKMPWIGRG"
     gene            7877..8086
                     /locus_tag="VGC82_13530"
     CDS             7877..8086
                     /locus_tag="VGC82_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424337.1"
                     /translation="MDRIKILDETLWPQVPKDVITEAEHAAKESTVWIARQGNGHIVA
                     MDGPGDPEVATGDITFIAEVAPSDG"
     gene            complement(8402..9484)
                     /locus_tag="VGC82_13535"
     CDS             complement(8402..9484)
                     /locus_tag="VGC82_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0424338.1"
                     /translation="MRAAVFRKGAIVVDTLPDPVPTEGQVLVRTLACGICGSDLHAAK
                     HTHQFVNLARRAGNRWAMDADRDVVFGHEFCCEVVDYGPSTQPRLAPGTLVCSMPMTL
                     AGETVQGIGYSNDIAGGFAQYMPLADRMLLPVPNGLPASSAALTEPIAVGWHAVQNAR
                     LAPDDVPLVVGCGPVGLAVIAGLVIKDVHPIIAADYSPARRALALKMGADIVVDPAEG
                     SPYEAWRQTATPDGYNPSRYAQLFGLGPRLRPAVIFECVGVPGVIQQVLEGAPPSARI
                     VVVGVCMETDRIEPFFGIVKQLNVQFVLAYTAQEFAESLHYIAEGKVDVSPLITGTVG
                     LDGVGGAFAELANPERHAKVLVDPWV"
     gene            complement(9503..10300)
                     /locus_tag="VGC82_13540"
     CDS             complement(9503..10300)
                     /locus_tag="VGC82_13540"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEY0424339.1"
                     /translation="MSGTSLLPWRRPHLNVIVLHGVIAARAGMLNADSVRPLIQKGFA
                     GSSRRPVILDIESPGGSPVQSDLIAGLIRDHADRTGVRVHAVIREVGASGGYWIACAA
                     DEIHANPMSIVGSIGVVGGGFGFPHLLDRLGIERRVYTAGTNKSRLDPFSPEKPEDVA
                     FAKDLMEKLHVRFKDWVRTRRRQRLKASDDSLFDGGYMLGEQALALGLIDGLSTVDEL
                     VRDLGGDRARARRIAPKRQRWALRLPRLALDTALDVLEDRAWRINLR"
     gene            complement(10297..11025)
                     /locus_tag="VGC82_13545"
     CDS             complement(10297..11025)
                     /locus_tag="VGC82_13545"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY0424340.1"
                     /translation="MPSITGPSFIGLADAEPPPIGPEVDATPRPLPSRSPIRGRYVTL
                     EPLHRRHANELWQAAHGADGSWTYLGVGPFASEQAMERQVMDLASVHDPMLWAVRPVS
                     TGVASGWLALMDIQPKNAAIELGNIWFSPRMQRTRAATEAIFLMLKLAADDLGYRRLV
                     WKCNALNGPSKRAAERLGFSYEGRHRMHIVVKGRQRDTDWYSIVGDEWLRCRDALLAW
                     LDPGNFAADGTALAGLRDLRGQPA"
     gene            11132..11602
                     /locus_tag="VGC82_13550"
     CDS             11132..11602
                     /locus_tag="VGC82_13550"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY0424341.1"
                     /translation="MMLRRVLLAAVLGAAFVSAASAATVSYTARLSGRAEVPKIDSAG
                     KGKLDATFDTQTKAFNYKLTFDGLSGPATAAHIHGPATRTQTAGVVAPLGDKSPTSPV
                     TGSVTLTDDQVKMLQSAKLYANVHTAANPGGEIRGQIRTVHPKKNAATTAATAK"
BASE COUNT         2164 a         3830 c         3696 g         2049 t
ORIGIN      
        1 gagctgctga cgcgccggcg gcctcggtga ccgtcgagcc gatccaggtc agaaatgtcg
       61 cgccggccag cacctatatc ggccgcgtac aggcgatcca gtcggttgcg atcgtcgcac
      121 gggtacaggc tttcgtggat aagatcgact tccaggaagg cgccatggtg aaggccgggc
      181 aacttttgtt cgagttgcag aaggcgccct atcaggctgc cgtggaagcc gcccaggccg
      241 ggctgcagcg agccgaggcg acgctgcgga acgcccaatt gatctatgaa cgcgactccc
      301 ggcttggccg aggagcggtc gtcagtcagt cacagatcga cgccgacctc gcgacccgcg
      361 acgcggataa cgcggacgtt ctgtcggccc gagccaatct ggaaacggcg gcgatcaatc
      421 tcagctacac cagcatcacc tcccccatag acggccggat cggcaaggcg gcctacaccg
      481 tcggcaatct cgtgggtgcc agcgccagcg ccggtccgct cgcgaccgtg gtacaggtgg
      541 acccgatccg ggtgatcttc tcggtggcgg accgcgatat cgtcagcgca ttgcagcgca
      601 cgcaccagac ctcggccaag atcgcgtcga cgctctcgct cgatctgcaa ctgccggacg
      661 gtcagcgtta cccgcagcac ggtgagatcg agttcatcga caacgcggtg gaccccgcca
      721 ccggaaccgt gagcgtttgg gggcgcttcg ccaatcccga gggcctgctg atccccggcg
      781 gcttcgtatc ggtggacgtc cagcgggcca cccccgagga gcggcccctc gtgccggtgc
      841 aagctgtgca ggacgacaag gctggacact tcgtcctgct ggtcgggccg gacaacaagg
      901 taagccagca gacgatctcg atcgaccggc agatcggcca ggactggatc gtcaccgccg
      961 ggctgaaggg cggcgagaac gtgatcatcg aaggactgca aaaggtgcag ccggggcagg
     1021 tcgtcagccc ggtgcaggct cagacccaaa acgagcagac cggcagcagc ccaaacggtt
     1081 cctcgaatgg cgcttcctcg catgccgcct cctcaaatgg cgtttcctcg aatggcgctt
     1141 cctctggcaa cgagcaggca tccgtggaca ctcgccccgg ccaggggcac tgagcggtgc
     1201 tgtcgagctt cttcatcgac cggccgaaat tcgcgatcgt catcgcgatc gtcatcacgc
     1261 tggcgggcct gttggcgctg tgggtgatcc cggtcgccca gtacccgaac atcaccccgc
     1321 cacagatcac cgtctccgcc aactacccgg gtgctgacgc cgagaccgtc gcgaacacga
     1381 tcggcatccc gatcgaggag caggtcaacg gggtgcagaa tgcgctgtat atgtccagca
     1441 ccagttcaag ttccggcagc tacacgctca cggtcacctt tgcgatcggc agcgacccga
     1501 acatcgacca ggtgaacgtg cagaaccggg tctcgctggc gcaggcgcag ctgccggcaa
     1561 cggttgccca gcaggggctg acggtgctgc agcaatccac caacttcgtc atcgcggtga
     1621 acctgttttc gccgaacaac aagtatgacc agacctttat ctccaattat gcgaacgtca
     1681 atctgcgata tccgctggcc cgcctgagcg gcgtcggcaa cgctcagatc ctgggcaacg
     1741 cccagtacgc catgcggatc tggatggacc cggtgcgcat gacttcgttg aacgtcagtc
     1801 ccagcgacgt ggtgaacgcg atctccacac agaaccagca atccgccgcc ggacagatcg
     1861 gggcgccgcc gatcagtggc ggccagcggc agcaactgac gatcatcacc caaggccggt
     1921 tggcttcggc cgaccagttc cgcgacatca tcgtcaagac aaacgccaat ggcggcattg
     1981 tacggatccg cgatatcgcc gacgtcgagc tcggggcgca gacctacgca tcgacgtcca
     2041 ggttgaatca gtttccatcg gcgaccctgg cgatctacca ggcgccggga gccaacgcgc
     2101 tggaactggc gaacgcgatc cagcagcagg tgaaggagat cgcgaaggac tttccgccag
     2161 gattggaata tgccgtggtt tacgattcca cgcgcttcgt tcgggccaat atctcggaaa
     2221 tcctcaccac gctggggata accctgcttc tggtgatcgc ggtcgtctac gtgttcctgc
     2281 aggactggcg cgccaccctg atcccaacct gcgcgatccc ggtttcgctg atcggcgtct
     2341 tcgcggtgct ctatatgtta ggctattccg ccaacacgat cgacctgttc gcaattgtac
     2401 tggcgattac tctcgtggtg gacgacgcga ttgtggtcgt ggaaaacgtc acccgccacc
     2461 tggaggagca cccgcagcag ccgccgcggg aggccacgcg tgaggcgatg gccgagatca
     2521 tcggtccggt catcgcgtcc acccttgtgc tggtggcggt cttcgccccg gtcggcttcc
     2581 tgccgggcat cagcggtcag cttttccgcc agttcgcggt caccatctcg gtctcggtgg
     2641 tgatttcggg cgtcaacgcc cttaccctca gtccggcatt gtgcgcgctg atgctgcgcc
     2701 cgccgaagca ggcaaggttc cgcctgttca ccgggttcaa ccgcgcgctg aattggacgc
     2761 ggggcgtcta tggcggcacc gtgcgctggc tgggcaggcg gctgctggtc ggcggcgtcg
     2821 cactgggcgg cgtgctggtc gctgcctacc tgctgttcgc cgcggtgctt gcgggcttcc
     2881 tgccgaatga ggaccagggc tacttcttcg tcaacgtcgg tctgccgaac ggggcggcgc
     2941 tggtgcagac cgagcaggtg atcgaccgga tcgggcagat ggtccgcagc acgccggggg
     3001 tgacggacgc gatcgagcta tccggtttca gcatcgtcaa cggaacgcag caaccgaacg
     3061 ccggcgccgt tatcgtaatc ctgaagccct gggaccagcg gaccgctgcc gacatgcggg
     3121 cggaggcaat catcggtcgc ttgcagacac agttcaatgc gcttccgaca gccaccatca
     3181 ccgcgttcaa tcctccatcg atcccggggc tgggccacac cggcggcctg gacctgcaga
     3241 tgcagggaag aaccggccaa tcctatccag acctggcggc aaccgcccgg gccctgatct
     3301 acgccggaaa ccaggaccca tcgctttcgt cggtcttcac cacgttctcg gcatccgtgc
     3361 cggaggtcat ggttcgcgtg aacacggccc gggcggaact tctgggtgtg tccccgaacg
     3421 atatctacac gacgctacag gcccatctcg gctcccaatt cgtgaactac ttcaacctgc
     3481 aaagccaggt gttccaggtc atcgtgcagg acgcgtcgcg cttccgggcg caggtctccg
     3541 acatcaacca gctttatgtg cgcagcgcat ccggcgcgat ggtgccgatg aacagcctga
     3601 tcgacatcac gacggttcag ggcagcaacg ccgtaacgcg ctacaacctt tatccgtcgg
     3661 tcgagatcaa tgggtcggcc cgcggcagcg cgagttccgg ccaggcgatg caggccatgg
     3721 cggcggtggc ggcacaacac ttgccgccgg gctacggcta cgaatggacc agcctgtcct
     3781 atcagcagca gcaaggcggc ggcgcggggg gcacggtatt cctgttcgcg ctggtgttcg
     3841 cctatctgtt cctcgtggcc cagtacgaga gctggtcact gccgatgtcg gtgatcctgt
     3901 ccgtatccgt cgcagcgttg ggcgccttgc tcgcgctttg ggtccggggc cttgcgctgg
     3961 acgtgtacgg gcaggtgggg ctggtgctgc tgatcgggct ggcggcgaag aacgcgatcc
     4021 tgatcgttga attcgccaag aaccgcgtgg accatgggga cgaggtgcgc gaagctgccc
     4081 aggccggcgc gacgactcgt ttccgcgcgg tgctgatgac tgcgattgcc tttatcatcg
     4141 gcgtgattcc cctggtgatc gccaccgggg ccggggccgg cgcaaggcag tcgattggaa
     4201 ccaccgtatt cggcggcatg gtcctggcga cgatcctggg catcgtctcg gtgccagtcc
     4261 tgttcatcat gttcgagttt ctggcgcggt ggagcggcca cctgttgcag cggagggaag
     4321 tccgcggcag accggcggaa tgaccgtgag caggtcgaac gcttgtcagc gcgaccgctt
     4381 gcaaaccctg gtggatggct cacgaccttt gagcaagatt tgccctcttg agccagactt
     4441 gccttcttga gccggactgg gacgtcaatt accagctgtc gttgacaaca acgcgccgcc
     4501 ggtgcgcatg gcagcgaacg attgccggcg gcctcttgtc aggtcccgtg tccccaccgg
     4561 catgtcgtaa ccctgcgggt tcggcatcga ccacatcgat gtaaccctgc cccgccttcg
     4621 cagggcatgc gcagcaaggt catcaccgtc tggatggcct gacctgcgcc cgtgcaactg
     4681 cctccaatgg ccaccaaagt ccgagcgacc attgattcag gccggcagcg gaaaccgctc
     4741 cagatacggc atgtagtcgg gctccacgtc gatttgcagg gtagccagaa cgcgaacgac
     4801 ggcgttgtag aatccaatcg ccagcgtcag gtccaccacc agttcgttgc ccaactcggc
     4861 ttgcagcgcg gtgaacgtgg cttcggtcat ggcgccatcg ttggtcattt cccgcgccga
     4921 tctcagcacc agcctgggga gcgaaccgag gcttgtgggt ttgccggcgg gctcgtcgat
     4981 cagcgcctga atgtcctcgt cgctgacgcc gaaatcatgg cccagcttga cgtggtgcga
     5041 ccactcgtag ggcgagcggg cgagccaacc gacttgcagg atagccagtt cgcgcaaccg
     5101 cggatcaagc ttgctgccgt agcggatgta cccaccgagg ccggaaaatg ctcgcgctgc
     5161 cttgggcgag ttcaccagcg ccttgaacag cgagatcggc cgcttaagca gatcctggtc
     5221 ttcgggtgcg aggtcggatg gatcgagata gggaacgcgg gccattggtt tcctccattg
     5281 cgtgcggcga cggaatgcgc gcaggttcgg tgtggcggct gcacgcaaaa tcaacaagat
     5341 acctgccgac cgaggggtca ataattcggc cggctgctat aacagtctgc cagaacggaa
     5401 atgaccactc ggcccgggga caccaacatg cgagccaccg tgacgccggc cggaaacctg
     5461 acgatggatg gcgacacatt cagggttgct ttgggttatg gcggcatccg cgccgacaag
     5521 cgggagggcg acggcgcgac gcccgccggt ctcttgccat tgcgcaccgt gctgtatcgc
     5581 ccggatcgac agccggcccc gatctgcgcc gtctcggtgc aaaggctcgc gccgtacgac
     5641 gggtggtgcg acgacccgac ccacgcggcc tataacagtt tggttcgctt gccgatcaat
     5701 gcccgcgccg aggcgttatg gcgcgaggat gcactctacg acatcatcgg cgtgcttggc
     5761 tggaacgatc ggccagtgga accagcccga ggttcggcaa tcttcctgca tgtcgcgagg
     5821 ccggatttcg cctcgaccga gggatgcgtg gcattgtcct tggaaaccct gctcgatgtc
     5881 ctgtcgaagg gcctgactga aatccttgtc acggtctgaa ccgttctgcc cttccctggc
     5941 attgcagcgc aagccggcag gcggagaaca cgcgcccgcc gtccagcgtc cggcccttcc
     6001 ggtcgcgcag gtcggccagg aaatcgctgt aatacgtcac aggcaggagg ctgcaatcct
     6061 ggtagcgcgt tcgttcccgg ttcgctacga acaaatggtc gccgcctgcc ctgccctcga
     6121 tgtccaggtt cttgcgtagt tccagggtcc cctgggtccc gaccaggaac aaccgcccgt
     6181 cgccccaatc cggcagccca tcgggcgtga accagtccat ccgcatcgtc ccggtcattg
     6241 tgccgctacg cattgttacc tccgcgaagt cttcgaaccc ctcaggcggg gttccgaaca
     6301 tcccgatcgt actcgcggcg atcgttgcct cggtggcgtc cgcgaaggcg aggaactggt
     6361 cgatcgagtg aaccccgata tcggtgatga tcccgccata ggcctcttgt tcgaagaacc
     6421 aggcgggacg cagcgcgcgg ttcagccgat gcggcgccag gctggtcagc gacaccagcc
     6481 gccccagttc acctgatcgg gcgactgcca gcgccgcctg caccgccggg gaggtcagac
     6541 ggcccaacgc gaccgaccag atccgccctg tttctcgtac gatagcctcc acggaggcca
     6601 actgatccaa ggtggtgaca cccggcttgt ccaccatgac atccttgccg cgctgcatgg
     6661 ccttgatcgc gaggccggcg cgatctcgtg gcacggcggc cagcacgatg aaatccaccg
     6721 aaggatcgtc gaacagccgg tcgcgcgtca ccgccggcac atcgggaaag cgcttgcgaa
     6781 agccggccag gacccgcggg tcggaggtct ccgggtcatg gccgacgcag gtcgcgcctg
     6841 cggccagtag cccggcggtc aggtcataga catggcggtg atccaggccg atcgcggcaa
     6901 agcgatgcat gtgcgtttcc atcggtcgaa gcgtcgcacc atcttccccg cgcggcaacc
     6961 gcggtccagg atagcgaggt gttcaattca agggggaaaa cccgatgaca gatcgtgtcg
     7021 ccattatcac cggcgcgggc agtggtatcg gacgcgctac ggcgctcaat ctgcttgccg
     7081 gcggctggca tgtcgcgctg gtgggccgcc gtcccgaggc actggaggaa accgccggcc
     7141 tggcgacagg aggcaagacg ctggtcgtgc cgaccgacgt caccgaaccg gccgccgtgg
     7201 acgcgctgtt cgccaaggtg aaatctacct atgggcgcct ggacacgttg ttcaacaatg
     7261 ccggcggcaa cgtccccacg acgaattttg gcgacttcac cttcgagatg tggcgcaaag
     7321 tctgcgcgat caacctggac gcgatgttcc tgtgcgccaa cgcggcgttc cgcgcgatgc
     7381 gcgaccagga gcctcagggc gggcggatca tcaacaacgg ctcaatttcg gcgcataatc
     7441 cgcggccggg gtcggtggcc tatacctcca cgaagcacgc ggtcaccggc ttgacgaagt
     7501 cgatcgcgtt ggatgggcgg caatacaaca tttgcagcag ccaagtggat atcggcaatg
     7561 ccgccacgcc gatgaccgcg cggtccacca acggccaaat gcagccgtat ggccagatgg
     7621 cggtcgagcc caggatggac gcggaccatg tcggcaagca ggtggcattc atggcgaacc
     7681 tgccgctgga atcgaacgtg cagttcgtga ccatcatggc caccaagatg ccttggatcg
     7741 gacgcggctg atgcaggccg ggcagcgggg ccattcgagc ttccctgccc gcttggttca
     7801 acgacgttgt ccggcagagg ggaaccgagt cgatccgctg gacgttcgaa ttgcgcgaga
     7861 taacggggag gatatcatgg accggatcaa aatcctggat gagaccttat ggcctcaggt
     7921 accgaaagac gtcatcaccg aggccgaaca tgccgccaaa gagagtaccg tctggatcgc
     7981 ccgccagggg aatggccata ttgttgcaat ggacggtccc ggcgaccctg aagttgcgac
     8041 aggcgatata acgttcattg ccgaggtcgc acccagcgac ggctaaggat tggtgacgaa
     8101 tatccgcttc gtggcgggtg atgcagcccc acaaagtcat ggggtgcttc gctccgtgta
     8161 cgccggaccg gttgctgtcg cgtggttgtc tgcgcttgaa acggggtcgg ccttatgccg
     8221 gccacccacg acttcccgcg ttaggacaaa tcgtgggtgg ccgcgccagg cgtggctatg
     8281 acactacgat agtgccttac cgcctgggaa ccccgccgac catgacacgt ggagacctct
     8341 gcccgaagcg gttattttgg atgccctgag cgcgagacct acctgttgac ccggcggaac
     8401 gctagaccca gggatcgacc agaaccttcg catgacgctc cgggttcgcc agttcggcga
     8461 acgcgccgcc aacgccgtcc aaaccgaccg tcccggtgat caacggagac acatcgacct
     8521 tgccttcggc gatgtagtgc aggctttcgg cgaactcttg ggccgtgtaa gccagtacga
     8581 actgaacgtt caattgcttg acgatgccaa agaacggttc gatccggtcg gtttccatgc
     8641 agacgccgac caccacgatc cgcgctgacg gtggcgcccc ttccagcacc tgctggatca
     8701 cccccggcac gccgacgcat tcgaagatca cggccggccg cagcctggga cccaggccaa
     8761 aaagctgggc gtagcggctg gggttgtatc cgtccggcgt cgccgtctgc cgccaggcct
     8821 cgtatggcga cccttcggcg ggatcgacga cgatgtcggc gcccatcttc agtgccaagg
     8881 cacgccgagc cggagagtag tccgcggcga tgatcggatg gacgtccttg atgaccaggc
     8941 cggcgatgac cgccaatccg accggaccgc aacccaccac caggggcaca tcgtccggtg
     9001 ccaggcgggc attctgcacg gcatgccaac ccaccgcgat cggttccgtc agcgcggcgc
     9061 tggacgctgg aaggccgttt ggaaccggca gcagcatgcg gtcggccagc ggcatgtatt
     9121 gggcaaatcc tccagcgatg tcgttggaat agccgatgcc ttgcacggtt tcgcccgcca
     9181 gggtcatcgg catcgagcaa accagcgttc ccggcgccaa tcgaggttgc gttgaggggc
     9241 cataatccac gacctcgcag cagaattcgt ggccgaagac cacgtcacga tcggcatcca
     9301 tcgcccagcg gttgccagca cgtcttgcca gattgacgaa ctggtgggtg tgcttggcgg
     9361 catggaggtc cgacccgcag atgccgcatg caagggtgcg taccagaacc tgcccctcgg
     9421 tcggaaccgg atcgggcagc gtatcgacga caatggctcc ctttcggaag acggctgcac
     9481 gcatggtggt gtttcctttt ctttatcgca ggttgatacg ccacgcccga tcttccaata
     9541 cgtccagcgc cgtgtccaaa gccagccgcg gcagccgaag cgcccagcgc tgccgcttcg
     9601 gcgctatccg gcgtgctcgg gctcgatccc cgcccaggtc gcgcaccagt tcgtccaccg
     9661 tcgaaagccc atcgatcaga ccgagcgcca gagcctgttc gcccagcata tagccaccgt
     9721 cgaacaagga atcgtcagac gccttcaggc gctgtctgcg gcgcgtgcgg acccagtcct
     9781 tgaagcgcac atggagtttc tccatcaggt ccttggcgaa tgccacgtcc tccggctttt
     9841 ccggactgaa cggatccaac cgagacttgt tggtaccggc ggtatagacg cggcgttcga
     9901 tgcccaatcg gtcgagcagg tggggaaagc cgaagccgcc gccgacgacg ccgatggagc
     9961 cgacgatgct catgggattg gcgtggatct catccgccgc acaggcgatc caatagccgc
    10021 cggacgcacc gacctcccgt atgacggcat ggacgcggac cccggttctg tccgcatgat
    10081 cgcggatcag gcccgcgata agatcggatt gcaccggcga tccgcccggg ctctcgatat
    10141 ccaggatcac cggccgccgg ctggagccgg caaagccctt ctggatcagc gggcgaaccg
    10201 agtcggcgtt cagcattccg gcgcgggccg cgattactcc gtgcaaaacg atcacgttca
    10261 ggtgaggacg acgccacggc agcaggctgg tgccgctcat gcgggctggc cccgcagatc
    10321 acgcaagcca gcaagcgcgg tcccgtcagc cgcgaagttc ccgggatcga gccacgccaa
    10381 caatgcgtcg cggcagcgga gccattcgtc gccgacgata ctgtaccagt cggtgtcgcg
    10441 ctgccgcccc ttcaccacga tgtgcatgcg gtgccggccc tcatagctaa agccaagccg
    10501 ctccgccgct cgcttcgagg gtccgttcag cgcgttgcat ttccaaacca atcgccggta
    10561 gcccaaatcg tccgccgcca gcttcaacat caggaatatc gcctcggtgg cggcccgcgt
    10621 gcgctgcatg cgcgggctga accagatatt ccccagctcg atcgcggcgt ttttcggctg
    10681 aatatccatc aacgccagcc acccggaggc gacgccggtg ctgaccggcc gcaccgccca
    10741 caacatcggg tcatgcaccg aagcgaggtc catgacctgg cgttccatcg cctgctccga
    10801 cgcgaatggc ccgacaccca ggtaggtcca ggatccgtcc gctccgtggg cggcctgcca
    10861 caactcgttg gcatgacggc gatgcaacgg ctcgagtgtc acatagcgac cgcggatcgg
    10921 ggaccgtgac ggcaatgggc gcggggtcgc gtcaacctcc ggcccaatag gcgggggttc
    10981 ggcgtctgcg agcccgataa atgacggtcc ggtgatcgaa ggcatggcgg catccttggt
    11041 ttcgtgagat acggtggccc cggcggcggc tgccgacaag ccgctaccgc cctggccgct
    11101 cgccggggca cgaaccacat attgggggac catgatgctc cgtagagttc ttctcgctgc
    11161 cgttctgggt gcggcttttg tttcggctgc ctcggccgcc acggtcagct ataccgcccg
    11221 actttccggc cgcgccgagg tcccgaagat cgacagcgca ggcaaaggca agctggacgc
    11281 cacgttcgat acgcaaacca aagcgttcaa ctacaagctg accttcgacg gcttgagcgg
    11341 cccggccacc gcggcccata tccacggccc ggcgacccgg acacaaaccg ccggcgtggt
    11401 ggctccactc ggggataaga gcccgaccag cccggtgacc ggttcggtta ccttgaccga
    11461 cgaccaggtc aagatgctgc aatcggccaa gctgtatgcc aatgtgcata ccgcggccaa
    11521 cccgggcggc gagattcgcg gccagatccg gaccgttcat cccaagaaga acgccgccac
    11581 cacggccgcg acagcgaagt aagcatccga ccctgtccca ccccggcgtc gcgccggggc
    11641 ggcaaccggt cacattcgcg gaccacaacg atgtccagca acggacgcat ccaggaggaa
    11701 cccatgatcc gtcgccgccg tctgcttcag gcttctgcc
//
LOCUS       DASWQN010000803         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2321078, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000803 DASWQN010000000
VERSION     DASWQN010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321078"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..575)
                     /gene="phnG"
                     /locus_tag="VGC82_13555"
     CDS             complement(78..575)
                     /gene="phnG"
                     /locus_tag="VGC82_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HEY0424342.1"
                     /translation="MNDNVSADHPHPAHPHPVDLHPGRRRWMAVLARGDASRLELLLR
                     DHGARPAYAVLRGPEGGLVMVRGKAGGGGASFNLGEMTITRCTVRTETGFIGHAYVAG
                     RDKKRAELAALADALMQDPDLAVDIERDIIAPLEWEQNARATRRAEKAAATRVQFFAM
                     QTMRT"
     gene            596..1435
                     /gene="phnF"
                     /locus_tag="VGC82_13560"
     CDS             596..1435
                     /gene="phnF"
                     /locus_tag="VGC82_13560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HEY0424343.1"
                     /translation="MSRQLDADLVIVNHELPWDGLGSYERMMNAASATDDPDTCLVHR
                     QEGVALWRQIVRQIQADIASGTSKPGARLPTEAELSVRFGVNRHTVRRALEELSRDGL
                     VRVEQGRGSFVAEDVLDYNVEARTRFSEWIRKHNKEPSGIIRQLREIPADQRVAAGLG
                     VRSGSRVVLLERLGLADDRPVSLARHYFPALRLKGMLQALQATPRITDALRAVGVEDY
                     LRQQTRVTARLPTPTEAELLRMARNRPVLITENINVDRTGAIVEFGSGCYPTPRVQIV
                     FEP"
     gene            1445..>1629
                     /locus_tag="VGC82_13565"
     CDS             1445..>1629
                     /locus_tag="VGC82_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424344.1"
                     /translation="MHDLRITGGTVLLGLDGLQAVDLVLKDGLIDKVGGAGGEASLDA
                     TELLVLPGIVDLHGDAF"
BASE COUNT          274 a          499 c          564 g          292 t
ORIGIN      
        1 catggcatcg agtacggcgc gaaagcacgc ttgggcactg gtgaccggat cggcgaatcc
       61 gggcgagagg tcgctgctca tgtccgcatc gtctgcatgg cgaagaactg cacccgcgtt
      121 gccgcggcct tctcggcacg cctggtcgca cgggcatttt gctcccattc gagcggggcg
      181 attatgtcgc gctcgatgtc aaccgcgagg tcggggtcct gcatcaaggc atcggccaag
      241 gcggccagtt cggcgcgctt tttgtcgcgt ccggcgacgt aggcatggcc gatgaagccg
      301 gtctcggttc gcaccgtgca ccgcgtgatc gtcatttcac ccaggttgaa cgacgcgccg
      361 ccaccgccgg ccttgccacg caccatcacc agaccgcctt ccgggccacg caacaccgcg
      421 taggccgggc gcgcaccgtg gtcgcgcaat agaagttcga gccggctggc gtccccccga
      481 gcgaggacgg ccatccagcg gcgacggccg ggatgcaggt cgaccggatg cggatgggcg
      541 ggatgcgggt gatcagcgga aacgttgtcg ttcatcgcgg tccggtcatg tttagatgtc
      601 tagacagcta gacgccgacc tggttatcgt caaccatgaa ctgccatggg acgggcttgg
      661 atcgtatgaa agaatgatga acgcggcatc cgccacggat gacccggaca cctgtctggt
      721 acatcggcag gaaggtgtgg cgctttggcg ccagatcgtc cggcagatac aggctgacat
      781 cgcgtccggc accagcaagc cgggcgctcg gctgccgacc gaggcggagc tttctgtgcg
      841 cttcggcgtc aaccgccata ccgtccggcg cgcgctggag gagttgtcgc gcgacggcct
      901 ggtgcgggtg gagcaggggc gtgggtcgtt cgttgccgag gatgtgctgg actacaacgt
      961 cgaggctcgg acccgctttt ccgaatggat acgaaaacat aacaaggaac cgtcggggat
     1021 catccgccag ttgcgggaaa tccccgcgga tcagcgggtc gcggccggcc tgggggtgcg
     1081 tagcggcagc cgcgtcgtgc tgctggagcg tctcggcctg gcggacgacc gccccgtcag
     1141 tctcgcacgg cactattttc ccgcgctccg ccttaagggg atgctgcagg cgctgcaggc
     1201 gacaccgcgc atcaccgacg ctttgcgtgc cgtcggtgtc gaagactacc tgcgccagca
     1261 gacccgggtt accgcccggc tgccgacccc aaccgaggcg gagctgctgc ggatggcgcg
     1321 aaacaggcct gtgctgataa cagagaacat caacgtggat cgcaccggag cgatcgtcga
     1381 attcggctcc ggctgctatc cgacgccgcg cgttcagatc gtcttcgaac cctgaggttt
     1441 atcgatgcat gacttgcgga tcactggtgg aaccgtgctg ctgggcctcg atgggctgca
     1501 agccgtggat ctggtgttga aggacgggct gatcgacaag gttggcggtg ccggcggcga
     1561 ggcttcgctg gatgccacgg agttgctggt gctgccgggg atcgtcgatc tgcatggcga
     1621 cgcgttcga
//
LOCUS       DASWQN010000804         3268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_342715, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000804 DASWQN010000000
VERSION     DASWQN010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342715"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VGC82_13570"
     CDS             complement(<1..880)
                     /locus_tag="VGC82_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424345.1"
                     /translation="MPEPLTDLDFAGVARFVALVGLKRWQQRLSEIRDLAASGVRAGQ
                     AIRQRHGVELSLEKLRRRSNAVPSMAEVLLGRIAGEIPQIAADLSPKGRERLIEKLRS
                     GLSGQNTLIPVFHLVRTAVAQRSRGFAVAFSGLDYETPYDLLLTRDQIEAEVACDVVT
                     AEEGRNVHRGAWFRLADRIDPDLQIWLTAHPGRYLLKMTLSDGLRGGLVDSDATLSRL
                     HGRIRTMLETHTRQDHDETIVLRLGPLLVAGAQAEDLGLISSLRREFGPEAHLSVTTA
                     GNAVFVMAARAGQENEV"
     gene            complement(1087..2205)
                     /gene="aroC"
                     /locus_tag="VGC82_13575"
     CDS             complement(1087..2205)
                     /gene="aroC"
                     /locus_tag="VGC82_13575"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY0424346.1"
                     /translation="MSHNSFGHLFRVTTWGESHGPAIGGVIDGCPPLLPLTEADIQPW
                     LDKRRPGQSKFTTQRQEPDAVRILSGVFEGMTTGTPISLVIENQDSRSRDYAAIATQF
                     RPGHADLTYDLKYGIRDYRGGGRSSARETAMRVAAGAVARLVLGGAVLGGAVRIRGAL
                     VQVGPHRIDRDRWDWGQVNQNPFFCPDSAAAAVWETYLSEVRKSGSSAGAVVEVVAEG
                     VPPGLGAPIYGKLDADLAGALMSINAVKGVEIGEGFAAAALSGEENADEMRMDDGLVA
                     FGSNHAGGILGGISTGQPIVARFAVKPTSSILSPRHSVDRLGQDIEIVTKGRHDPCVG
                     IRAVPVGEAMVACVLADHLLRHRAQNPHAPTTTRLPRN"
     gene            complement(2217..3059)
                     /gene="fabI"
                     /locus_tag="VGC82_13580"
     CDS             complement(2217..3059)
                     /gene="fabI"
                     /locus_tag="VGC82_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY0424347.1"
                     /translation="MTDSAPAPLPAAGSLMRGKRGLIMGVANERSLAWGIAAACAAQG
                     AELAFTFQGEALERRVRPLAAQTGSNLVIPCDVADEASIDSAFAALRDRWDGLDFLVH
                     AIGYADKQYLRGRYLDTPRDAFLQALDISCYSFTSVCQRAVPMMKPGGSLLTLTYLGA
                     ERWVPHYNVMGVAKAALEASVRYLAADLGAAGIRCNGISAGPIKTLAASGIGDFRYIL
                     RWNQLNSPMRRNVTIEEVGGAGVYLLSDLASGVTGEIHHVDCGYHTVGMKHPDAPDIA
                     VAEG"
     gene            3058..>3268
                     /locus_tag="VGC82_13585"
     CDS             3058..>3268
                     /locus_tag="VGC82_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424348.1"
                     /translation="MMILSVAMREGMRRFELVAQGSRTRQGVVHADSTETGSRQALAA
                     LVAAAVTGSRGAFATDNGFRRAADGK"
BASE COUNT          598 a         1067 c         1036 g          567 t
ORIGIN      
        1 ccacctcgtt ctcctgaccg gcgcgcgctg ccatgacgaa aactgcgttg ccggccgtgg
       61 ttaccgacag atgcgcttca ggcccgaatt cgcgacgcaa cgatgaaatc aggcccaaat
      121 cctcggcctg tgcgccggcc accagcaatg ggccgaggcg caggacgatc gtttcgtcat
      181 ggtcttgccg cgtatgggtc tccagcatgg tgcgaatgcg cccgtgcagc cgggacagcg
      241 tcgcgtctga atccaccagt ccgccgcgca atccatctga cagggtcatt ttcagcagat
      301 aacggccggg atgagcggtc agccagatct gcagatccgg atcgatccgg tcggccagcc
      361 ggaaccaggc accgcggtga acattgcggc cttcttcggc ggtcaccacg tcgcaagcca
      421 cctccgcctc gatctggtcg cgcgtcagca gcaggtcgta aggcgtttcg taatcaaggc
      481 cggaaaatgc gacggcgaac ccgcgggagc gctgcgccac ggcggtgcgc acaaggtgaa
      541 aaaccgggat cagagtgttc tgcccgctga ggccgcttcg aagcttttct atcagccgct
      601 cgcggccctt cggcgaaaga tccgctgcga tttgcgggat ttctccggcg atgcgcccca
      661 gtaagacctc cgccatggag gggaccgcgt tcgagcggcg gcggagcttt tccaggctga
      721 gttcgacgcc gtgccgctgc cggattgcct ggccggctcg cacgcccgaa gcggcaagat
      781 cacggatctc cgataaccgc tgctgccacc gctttaagcc taccagagcg acaaaccgcg
      841 caactccagc aaaatccagg tcggtcaacg gctcgggcat ctcagatcag gtcatgagtg
      901 ggagtagccc attcatcact caatattgcg gtgcgtcaag tcgaagtgtg gcacatcgcg
      961 gcgtcatgat gcgagccgtc gtgaaacgtc atggccgccc gctccggcgc ggcctcccca
     1021 tccccagggc catccccttg ggtccgtccc ttgggtccgt tgcacaatac aacgaacttc
     1081 gcgacatcaa ttgcgcggca accgggtggt ggtaggtgcg tgtggatttt gcgcccggtg
     1141 acgaagcagg tgatcggcca gcacacaggc gaccatggcc tcacccactg gaaccgcgcg
     1201 aatgccgaca caaggatcgt gccgaccctt ggtgacgatc tcgatgtcct ggccaaggcg
     1261 gtccaccgag tggcgcgggg acaggatcga gcttgtcggc ttaacggcga aacgggccac
     1321 gatgggttgg ccagtcgaaa tgccgccaag gatcccgccg gcatggttgg agccgaatgc
     1381 caccagccca tcgtccattc gcatttcgtc ggcattttcc tcgccggata gagcggcggc
     1441 ggcgaagcct tcgccgattt ccacgccttt gacggcgttg atcgacatca gcgcgcccgc
     1501 gagatcggcg tccagcttcc cgtagatcgg agcgccgagg cctggcggaa cgccttcggc
     1561 cacgacttct acaaccgccc ctgccgatga accggatttg cgcacttccg acaagtaggt
     1621 ttcccaaact gccgcggcgg cggagtccgg gcagaaaaac gggttctggt tcacctgccc
     1681 ccaatcccag cgatcccggt cgatccggtg cggcccaacc tgcacaagcg cgccccgaat
     1741 ccgtactgca ccgcccagca ctgcaccgcc cagcacgagg cgcgcgaccg cacctgctgc
     1801 gacccgcatc gcggtttccc gagcagacga gcgcccgccg ccgcgataat cccggatgcc
     1861 gtatttcagg tcgtaggtca ggtcggcatg accgggccgg aactgcgtgg cgatcgccgc
     1921 gtagtcgcgt gaccgagagt cctggttctc gatcaccagg ctgatcgggg taccggtcgt
     1981 catgccttcg aaaacgccgg acaggatgcg gaccgcatcg ggttcctggc gttgcgtggt
     2041 gaatttcgat tgccccggcc gccgcttatc gagccagggc tggatgtcgg cttctgtcag
     2101 cggcagcaac ggggggcagc cgtcgatcac cccgccgatc gctggaccgt gactttcacc
     2161 ccacgtcgtg acccggaaaa gatgcccgaa gctgttatgc gacatggtgg cggctgtcag
     2221 ccttccgcga cggcgatatc gggggcatcg ggatgtttca tccccacggt atgatacccg
     2281 caatcgacgt ggtggatttc tccggtcaca ccggaggcga ggtcggatag cagatacacg
     2341 ccggcgccgc cgacttcctc gatcgtcaca ttgcgccgca tcggtgagtt gagttggttc
     2401 cagcgcaaga tgtagcggaa atcgccgatg ccggatgcgg ccagggtctt gatcggaccg
     2461 gcgctgatcc cgttgcagcg tatgccggcg gctcccaggt cggcggccag ataacggacc
     2521 gatgcttcga gtgcggcctt cgcgacgccc ataacgttgt aatgcggcac ccagcgttca
     2581 gccccgagat aggtcagcgt cagcaggctg ccgccgggct tcatcatcgg caccgcccgc
     2641 tggcagaccg aggtgaagga ataacacgaa atgtccaatg cctgcaggaa cgcgtcccgc
     2701 ggtgtgtcca agtaacggcc gcgcaggtac tgcttgtcgg catagccgat ggcgtggacc
     2761 aggaagtcca gcccgtccca acggtctcgc aatgcggcga acgccgaatc gatgctcgcc
     2821 tcatccgcga catcgcaggg gatcacgaga ttcgatccgg tttgggccgc cagcggacgc
     2881 acccggcgct ccaatgcttc accctggaaa gtgaacgcca gttcagcgcc ttgtgcggcg
     2941 caggcagcgg cgattcccca agccagcgaa cgttcgttgg cgacgcccat gatcaggcca
     3001 cgcttgccgc gcatcaggct gcctgcggcg gggaggggtg ccggagcact atcggtcatg
     3061 atgatcctct cggtggcgat gcgcgaaggc atgcgccgct tcgagttggt ggcacaaggc
     3121 agccggaccc ggcaaggggt tgttcatgcc gattcaaccg aaaccggctc ccgtcaagcg
     3181 ttggccgctc tcgtggccgc cgcggtaacc ggcagtcgag gcgctttcgc gaccgacaac
     3241 ggcttccggc gggcggcgga cggcaaga
//
LOCUS       DASWQN010000805         5587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_474103, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000805 DASWQN010000000
VERSION     DASWQN010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474103"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VGC82_13590"
     CDS             <1..1046
                     /locus_tag="VGC82_13590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0424349.1"
                     /translation="VEELTRAGFLGALIPEEYGGSGLPVRAAAVILEEINASGCVASQ
                     CHAQMYIMGTLLRHGSEAQKRKYLPEIAAGRIRLQAFGVTEPTTGSDTTQLKTRAVRD
                     GDSYVVNGQKVWTSRAMHSDMMLLLARTTSADQVKKRSDGLSVFLIDMTAKNTGSGLE
                     VRPIKAMINHNTTEVFFDNFRIPADSLIGEEGKGFRYILDGMNAERILVASEAVGDGR
                     WFVRKATQYAKDRVVFGAPIGKNQAVQFPIARAWAELEAADMICRRAAALFDNGQECG
                     ADANIAKLLASESAWKAADTAMQTFGGFAYAREYEIERKWREVRLYQIAPISTNLILG
                     YVGQHVLGMPRSY"
     gene            complement(1040..1591)
                     /locus_tag="VGC82_13595"
     CDS             complement(1040..1591)
                     /locus_tag="VGC82_13595"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0424350.1"
                     /translation="MSATVQKRMTADEFIAWAMEQPEGKRYELVAGEVVAMAPERAAH
                     GRMKGRIFVRLLDAIRVAHAECEAFPDGMAVRVDANTLYEPDAMVRCGAPVDDNATEV
                     ADPLIVVEVVSPSSRKRDSGGKLEDYFRIPSVRHYLIVKTENRTIIHHRRDEAGEIAT
                     HIIRDGDIRLDPPGLVVTGLFVQ"
     gene            1733..3319
                     /locus_tag="VGC82_13600"
     CDS             1733..3319
                     /locus_tag="VGC82_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424351.1"
                     /translation="MPRSRLWLALACLVASTVAASAQKQGGTLRITHRDNPPSASVLE
                     EATISTLMPFMAVFNNLVVFDPESPQNRMDKVVPDLATSWALTEDGRTLTFQLRGGVK
                     WHDGKPFTSADVKCTFDILSGKASAKLRKNPHKAWWFNIKDIVANGDEQVVFHLNDPQ
                     PSIMAMFAGGFSPIYPCHVPAAEMRTRPIGTGPFKFAEFKQNESIRLVRNPDYWKQGR
                     PYLDAIEYTIIPNRATMVLAMATGKFDLSFTSELTPELTKSLQEQAPAMQCVLQPTGT
                     QGNLLVNRERAPFDNEQVRKAMVLSIDRRAFSDILSRGVFEVGGANQPPPEGLWGWSK
                     AFTETVPGYGADVEKSREEGRQIMKSLGYGPDKMLALKVSTRNLPDYRDAAVILIDHL
                     KSIYIQGELEPLDSSVWYARLARKDFSVGMNVQGMSIDDPDVVFFENFACGSERNYTA
                     YCNADLEKKFHEQSAMKDVDARRKLVWDIDRALQVDAARPVVYHAKAGTCWWPRVHGI
                     RIATNTIYNHWRFEDVWLDQ"
     gene            3326..4273
                     /locus_tag="VGC82_13605"
     CDS             3326..4273
                     /locus_tag="VGC82_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0424352.1"
                     /translation="MFEYILRRLGLMVLTLFGVSVAIFLLLRIVPGNIADILFDSAGL
                     IDPAEKQQIVKDLGLDLSLVQQYIHWIAGLARGDLGYSYVSEKPVLDEILPRIPITAR
                     LAGLALLFAVCTGVPFGVISAVRQNTRLDYVLRVFSLLFLSLPSFWLGLLVLMAFVNW
                     FGLLPIYEKAPQTWGAFVLALCVPAATVGLRSSALIMRLTRSSMLEVLRQDYVRTARS
                     KGLSEPAVTFIHALRNAVLPVITTIGIETAFLFGGLIVTETVFNIPGVARFLVEAIRW
                     RDYPMVQNLVMFIAIIVVTVNLIVDLAYMFVDPRIRLRA"
     gene            4270..5103
                     /locus_tag="VGC82_13610"
     CDS             4270..5103
                     /locus_tag="VGC82_13610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0424353.1"
                     /translation="MREALYYARRYPLGVAGAVILIVFVLTAIFAGVVAPFDPLTTNA
                     RASLAPPSGTYLIGADMMGRDMLSRMIHGARISLAVALGATCIGSGLGAMLGLASGYL
                     GGWFDLIVQRVAEVMQSLPLLVMALVITASLGPSLTNTIIAIAIPLIPNVARVIRANV
                     LSLRELNYVEAARAVGMRQTRIALVHVFPNTLAPLIVLATAQLGSTILTEASLSFLGL
                     GVPEPYPSWGRMLSESAAEYIRSAPWLVIFPGLAISLVVFGANLFGDALRDILDPRQG
                     D"
     gene            5103..>5587
                     /locus_tag="VGC82_13615"
     CDS             5103..>5587
                     /locus_tag="VGC82_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0424354.1"
                     /translation="MAPVLEVSGLSTVFHTRTGIVRAVQDVSFSVEAGETLAIVGESG
                     CGKTMTALSLMRLIPTPPGEISAGTVKLDGVDLLALDEASMRKMRGSQIAMIFQEPMT
                     ALNPLFTVGRQIAEMVSLHENVSRRAANARAVEMLHQVRIPAPERRAREYPHQLSGGM
                     R"
BASE COUNT         1005 a         1740 c         1754 g         1088 t
ORIGIN      
        1 tcgtggagga actgacccgc gccggtttcc tcggtgcgct gattccggag gaatacggtg
       61 gctccggcct gccggttcgc gccgcagcgg tcattctgga ggaaatcaac gcctcgggct
      121 gcgtcgccag ccagtgccac gcgcagatgt acatcatggg cacgctgctg cggcatggca
      181 gcgaagcgca aaagcgcaaa tacctgcctg aaatcgcggc tggccgcatc cggctgcagg
      241 cgttcggcgt gaccgaaccc accaccggtt ccgatacgac gcagttgaaa acccgagcgg
      301 tgcgggacgg cgattcgtat gtggtcaacg ggcagaaggt atggacgtcg cgcgccatgc
      361 actccgacat gatgctgctg ctggcgcgca cgacatcggc cgaccaggtg aagaagcgca
      421 gcgacgggct gtcggtcttc ctgatcgaca tgaccgcgaa gaacaccggg tcggggctgg
      481 aggtccggcc gatcaaggcg atgatcaacc acaacacgac cgaggttttc ttcgataatt
      541 tccgcattcc ggctgacagt ctgatcggcg aggaagggaa ggggttccgc tacatcctgg
      601 acgggatgaa tgccgaacgc attctggtcg ccagcgaggc ggtcggcgac ggccgctggt
      661 tcgtgaggaa ggctacgcaa tacgccaagg accgggtcgt cttcggcgcc ccgatcggca
      721 agaaccaagc ggtccaattc ccgatagccc gagcctgggc ggaactggag gcggccgaca
      781 tgatatgccg ccgggccgcg gcgctgttcg acaacggtca ggaatgcggc gcggacgcca
      841 acatcgccaa gttactggct tcggaatcgg cgtggaaggc cgccgacacc gcgatgcaaa
      901 cctttggcgg ttttgcctat gcaagggaat acgagatcga gcggaagtgg cgcgaggtgc
      961 ggctgtacca gatcgcgccg atctcgacca acctgatcct gggttatgtg ggtcagcacg
     1021 tccttgggat gccgcgatcc tattgaacga acagccccgt cacgacgagg cccgggggat
     1081 cgagcctgat atcgccatcg cgaatgatgt gggtggcgat ctccccggcc tcgtcacgtc
     1141 ggtgatggat aatcgtacga ttttcggttt tgacgatcag atagtgccgc accgagggaa
     1201 tgcggaaata gtcctcgagc ttgccgccgg agtcgcgttt gcgggaggaa ggggagacca
     1261 cctcgacaac gatcaacgga tcggcgacct ccgtcgcgtt gtcatcgaca ggcgcgccac
     1321 agcggaccat cgcatcaggt tcgtaaagcg tattcgcatc cacccgtacc gccatcccgt
     1381 cgggaaatgc ttcgcattct gcatgggcca cacgtattgc atcgaggagt cgcacgaata
     1441 ttcggccctt catccggccg tgcgccgcgc gctccggcgc catggctacc acctcgccgg
     1501 cgacgagttc gtagcgcttg ccctccggct gttccatcgc ccaggcgatg aattcgtccg
     1561 cggtcatgcg tttctggacg gtggcgctca tggtgtaagc ctggactgcc aatgaacatc
     1621 aatgatgggc tctccgaacg agtggcgcaa ggatggaccg atgtcattcc caccgcgccg
     1681 atcgttcgct aaggtgccgc cagtaccaag aatgcccgaa gggaaaacgt ctatgccgcg
     1741 atctcgccta tggttggcgc tcgcttgcct ggttgcttcg accgtggccg cgtcggccca
     1801 gaagcagggc ggcacgttgc gcatcacgca tcgcgacaac ccgcccagcg cctcggtgct
     1861 ggaagaggcg acgatttcca cgctgatgcc gttcatggcg gtgttcaaca acctggtggt
     1921 gttcgatcct gaaagcccgc agaaccggat ggacaaggtt gtgcccgacc ttgccacgtc
     1981 gtgggcgttg accgaggacg gccgcaccct gacgttccag ttgcgcggcg gcgtcaagtg
     2041 gcacgacggc aagcccttca ccagcgccga cgtgaaatgc acattcgata ttctgtccgg
     2101 caaggcaagc gccaagttac gcaagaaccc gcacaaggcc tggtggttca acatcaagga
     2161 catcgtcgcc aacggtgacg aacaggtggt gtttcacttg aacgacccac agccgtcgat
     2221 catggcgatg ttcgccggcg gattttcgcc gatctatccc tgccatgtac cggccgccga
     2281 aatgcgcacc cgtccgatcg gcaccggacc gttcaagttc gccgaattca agcagaatga
     2341 gtcgatccgc ctggtgcgca accccgacta ctggaagcaa ggccggccat acctcgatgc
     2401 gatcgaatac accatcatcc ccaaccgggc gacgatggtg ttggcgatgg cgaccggcaa
     2461 gttcgacctg tcgtttacca gcgaactgac ccctgaactg acgaaaagcc tgcaggaaca
     2521 ggcgccggcg atgcagtgcg tgctgcaacc cacgggcacc cagggcaacc tgctggtcaa
     2581 ccgcgaacgc gccccgttcg ataacgagca ggtccgcaag gcgatggtcc tgtccatcga
     2641 ccgccgagcg ttctccgaca ttctcagccg tggtgttttt gaggttggcg gtgccaacca
     2701 gccgccgccc gaaggcctct ggggctggtc caaggcgttc acggaaactg tccctggata
     2761 cggtgccgat gtcgagaaaa gccgcgagga aggccgccaa atcatgaagt ccctcggcta
     2821 cggcccagac aagatgctgg ctctgaaggt gtccacgcgg aacctgccgg attatcgcga
     2881 tgcggcggta attttgatcg accacctgaa gtcgatctac atccagggcg aactcgaacc
     2941 gctggattcc tcggtctggt atgcccgatt ggctcgcaaa gacttctctg tcgggatgaa
     3001 cgtgcagggc atgagcatcg acgacccgga cgtggtgttc ttcgaaaact tcgcatgcgg
     3061 ctccgaacgt aactacacgg cgtattgcaa cgccgacctg gagaagaaat tccatgagca
     3121 gtcggcgatg aaggatgtcg atgctcgccg caagctggtt tgggatattg acagggcgtt
     3181 gcaggtcgat gccgctcgcc cggtggtgta ccacgccaag gccggcacct gttggtggcc
     3241 gcgcgtgcat ggcatcagga tcgccacgaa cacgatctac aaccactggc gattcgagga
     3301 cgtgtggctg gaccagtaaa ccccgatgtt tgagtatatc cttcgccgcc tcgggctgat
     3361 ggtgctgacc ctgttcgggg tgtcggtcgc catattcctg ttgctgcgca tcgttcccgg
     3421 caacatcgcc gatatcctgt tcgactcggc cgggttgatc gacccggcgg agaagcagca
     3481 gatcgtcaag gatctcggcc tcgacctgtc gctggtgcag caatacatcc attggatcgc
     3541 ggggctggcc cgcggtgacc tcggctattc ctatgtctcg gaaaagcccg tgctggacga
     3601 aatccttccg cgtatcccga tcaccgccag gttggcaggc ttggcgctgt tgttcgccgt
     3661 ctgcaccggc gtgccgttcg gcgtcatcag cgccgtccgg cagaacaccc ggctggatta
     3721 tgtcctgcgg gttttcagcc tgctgttcct gtccctgccc tcgttctggc ttgggcttct
     3781 ggtgctgatg gcgttcgtga actggttcgg cctgctgccg atctacgaaa aggcgccgca
     3841 gacatggggg gcgttcgtcc tggcgctgtg cgttcccgca gccaccgtcg gcttgcgcag
     3901 ctccgcgctg atcatgcgcc tgacgcggtc gtcgatgctg gaagtcctgc gccaggacta
     3961 tgttcgcacc gctcggtcga aaggcctgtc cgaacccgcc gtcaccttca tccacgcgct
     4021 gcgcaacgcg gtgctgccgg tgatcacgac gatcgggatc gagaccgcgt tcctgttcgg
     4081 cggcctgatc gtcaccgaaa ccgtgttcaa catccccggt gtcgcccggt tcctggtgga
     4141 ggcgatccgc tggcgcgact acccgatggt gcagaacctg gtcatgttca ttgcgatcat
     4201 agtcgtgacc gtgaatctga tcgtcgatct ggcctacatg ttcgtcgatc ctcgcatcag
     4261 gctgcgcgca tgagagaggc cctgtactat gcccgccgct atccgctggg cgtcgccggc
     4321 gcggtcatcc tgatcgtctt tgtcctgacg gcgattttcg ccggcgtggt ggcaccgttc
     4381 gatccgctga cgaccaatgc cagggcgtcc ctggcgccac cgagcgggac atacctgatc
     4441 ggcgccgaca tgatgggccg ggacatgctg agccggatga tccacggcgc ccgtatctcg
     4501 ctggccgtcg cccttggcgc cacctgtatc ggcagcggcc tgggcgcgat gctggggctt
     4561 gcgtcgggct acctcggcgg ctggttcgac ctcatcgtgc aacgtgtggc cgaggtcatg
     4621 cagtcgctgc ctctgcttgt gatggcgctc gtgatcaccg cttcgctggg tccgtcgctg
     4681 accaacacga tcatcgccat cgccatcccg ttgatcccaa acgtcgcccg cgtcattcgt
     4741 gccaacgtgc tgtcgcttcg cgagctgaac tatgtcgagg cggcgcgtgc cgtcgggatg
     4801 cgccagaccc gtatcgccct ggtgcatgtc tttcccaaca ccctcgcacc cttgatcgtg
     4861 ctggcaacgg cgcagcttgg atcgaccatt ctgacggagg catcgttgtc gtttctcggc
     4921 cttggcgtgc cggagccata tccctcgtgg ggccgcatgc tgtctgaatc ggcggcggaa
     4981 tatatccggt ccgctccctg gctggtgata tttcccggcc tggcgatcag cctggtggtg
     5041 ttcggcgcca acctgttcgg cgatgcgctg cgggacatcc tcgatccgag gcaaggcgac
     5101 tgatggcccc cgtcctggaa gtgtccggct tgagtacggt ctttcatacg cgaacgggca
     5161 tcgtccgcgc ggtgcaggac gtttcattca gcgtcgaggc cggcgaaacc ttggctatcg
     5221 taggagaatc cggttgcggc aagaccatga ccgcactgtc gctcatgcgc ctgatcccga
     5281 cgccgccggg tgagatctcg gcgggaacgg taaagctgga cggtgtcgac ttgctggcgc
     5341 tggacgaggc cagcatgcgg aagatgcgcg gcagccagat cgcgatgatt ttccaggagc
     5401 cgatgacggc gctcaacccg ctgttcaccg ttgggcggca gatcgccgag atggtctcgt
     5461 tgcatgaaaa tgtttcccgt cgcgccgcca acgcccgcgc cgtggagatg ctgcaccagg
     5521 tacgcattcc cgccccggaa cggcgggccc gcgaatatcc gcaccagctt tccggcggca
     5581 tgcgcca
//
LOCUS       DASWQN010000806         4816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_210982, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000806 DASWQN010000000
VERSION     DASWQN010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210982"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1118
                     /locus_tag="VGC82_13620"
     CDS             219..1118
                     /locus_tag="VGC82_13620"
                     /inference="COORDINATES: protein motif:HMM:NF005861.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0424355.1"
                     /translation="MSLLCSGRVAIVTGAGRGIGREHALALAAHGAKVVVNDLGTSGD
                     GVGADETPAQEVVRTIIERGGDAVVNTDNVADFRGAKRLIDQAIDTYGTLDVLVNNAG
                     ILRDRVLVNMVEPEWDAVIEVHLKGTFAPSRHASAYWRNKSKEIDGPVNARIINTSSV
                     SGLYGNTGQTNYGAAKAGIAAFTVIAARELKRYGVTVNAVAPQALTRMTEGLRERTDE
                     QKSAMHPGRVSPAIVWIASAESADFTGRIVEAGGGLLAIAEGWHRGPTVPPEEDPAKL
                     GPIMMELQARARKNAGMNGMDLD"
     gene            1126..2328
                     /locus_tag="VGC82_13625"
     CDS             1126..2328
                     /locus_tag="VGC82_13625"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0424356.1"
                     /translation="MTRALFAGLKVIDCASFVAAPAAATVLADFGAEVIKIEPPGDGD
                     PWRTLYKRPGMPASEHNHPWLMDCRNKLGLGLDLKSEAGRAVLERLVSQADVFITNVP
                     LPARERLRIRYADFADKYPRLIYASLTAYGETGEEAGKTGFDATAYWARSGLMDEVRP
                     DHRAIPARSVAGMGDRPTAMALYGGIVTALYQREKTGLGGEVRASLMANGMWANGYLI
                     QARLCGVDFPPRPPREESVSALGNIYRTSDDRWFMLAVLNERQWPALARAVGMPELAL
                     DPRFAQPRERGVNAVVLMGILDEVFARRPLAEWRTILDAAGLTFGVVGTVAEIATDPQ
                     AVAAGILRPLGDIGMMTVDSPFTLSAAEKVPVEVAPGYGEHSRAILLAAGYSETEVSD
                     LRSSGTIV"
     gene            complement(2353..3132)
                     /locus_tag="VGC82_13630"
     CDS             complement(2353..3132)
                     /locus_tag="VGC82_13630"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0424357.1"
                     /translation="MVLTNQVALVTGAGQGTGKAAALALAAAGASVVVADIAARKAEE
                     TAETIISLQQRAAAVSADVGDLGDIDRMVRRALGLFGQIDILVNNAGVTRRADIMDLT
                     EADWDRIHRVNAKGVFFCLQRVAREMIPQRSGRIINIASIAGKGYVGTSNAAYAASKG
                     AVISLTKTAAQQLGRYNINVNSVCPGVTRTALSDANLHIRAQQEGISLEEVERRRAEV
                     IPLRRANDPEDIAAMVVFLASSGARNITGQSFNVDGGLIMD"
     gene            complement(3214..4443)
                     /locus_tag="VGC82_13635"
     CDS             complement(3214..4443)
                     /locus_tag="VGC82_13635"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0424358.1"
                     /translation="MSDNSQDLDFLAGIRVVDFTQFEAGPSCTETLAWLGAEVVKIEN
                     PKTGDPGRRLRPGQPDDDPYYFHMFNANKKSITVNLKSPQGLALVKDLLRKADVCVEN
                     MAPGTIERLGLGYDVVRELNPGIIYCQVKGFGTGSPYETNLAFDMIAQATGGTISVTG
                     ERGRQPVKPGLSLGDTGTGMTMAITILGALHKRAKTGEGHRLQVAMQDAMLHYMRTNF
                     STQARSGKAVERDGTRSGGGSNAPSGLYACAPGGPNDYVWIMTSRANPEHWSRLCKVM
                     GREDLITDPRFATPADRVKHDAVLDAIISEWTRTRTKHEAMAQVGGAGIPAGAVLDTM
                     ELQNDPTFEERGIMQVMQHPKHHPFKMPAWPVRVDGKPPRVSASPMLGQHTDDVLTNW
                     LGLSHAEVEKLRADSVV"
BASE COUNT          844 a         1514 c         1560 g          898 t
ORIGIN      
        1 atttggcacg cggcagcctt accaacctgc gcgccaaccg gtcagccttc cgggggcttt
       61 ctgccggtgc cgattcgaac agcgaaaagt gcctttgcat cgatccgcgg tagagaccga
      121 tccccaaggc cggcctgtat gttgacctgg gtgggcaagt cacccgaaac tcgggaaggg
      181 ccgcgtttgg tccgtcggca attcaagggg aacagttcat gagtctgctg tgcagcgggc
      241 gtgttgcgat cgtcaccggc gcggggcggg gtatcggccg ggagcatgcg ttggcgctgg
      301 ccgctcatgg cgcgaaggtc gtggtcaacg atctcgggac cagcggagac ggcgtcggag
      361 ccgatgaaac gcccgcgcag gaggtcgtgc gcacgattat cgaacgcggc ggcgatgcgg
      421 tggtgaacac cgacaacgtc gccgacttca ggggcgccaa gcggctgatc gaccaggcga
      481 tcgacaccta tggaacgctg gatgtactgg tgaacaatgc cggtattctg cgcgaccgcg
      541 tgttggtgaa catggtggaa ccggaatggg acgcggtgat cgaggtgcat ctgaaaggca
      601 cgttcgcgcc ttcacgccat gcctcggcgt attggcggaa caagtccaag gagatcgacg
      661 ggccggtcaa cgcccgcatc atcaacacca gttcggtgtc cggcctgtac gggaataccg
      721 gccagaccaa ttacggtgcg gccaaggccg ggatcgcggc gttcacggtg atcgccgcgc
      781 gggaactgaa acgctatggc gtgacggtga atgccgtcgc tccgcaagcg ttgacgcgga
      841 tgaccgaggg actgcgggaa cggaccgatg agcagaaatc cgccatgcat cccggccgtg
      901 tttcccccgc catcgtctgg atcgccagcg ccgagtcggc ggacttcacc ggccggatcg
      961 tcgaagccgg cggcggtttg ctggccatcg ccgaaggctg gcaccgcggc ccgaccgtgc
     1021 caccggaaga ggatccggca aaactcgggc ccatcatgat ggagttgcag gcccgggccc
     1081 ggaagaacgc agggatgaac ggcatggacc tcgattgacc ccgtcatgac ccgggctttg
     1141 ttcgccggat tgaaggtcat cgactgcgcg agctttgtgg ctgcccccgc ggcagccacc
     1201 gtgctggcgg attttggcgc cgaggtcatc aagatcgagc cgccggggga cggcgatccc
     1261 tggcggacgc tgtacaagcg cccgggaatg ccggcatccg aacataacca tccttggttg
     1321 atggactgcc gcaacaagct cggcctcggt ttggacctga aatccgaagc cgggagggcg
     1381 gtgctggagc gactggtttc ccaagccgac gtcttcatca ccaacgtgcc gctgcccgcg
     1441 cgggagcggt tgcggatccg ttacgccgat ttcgccgaca aatacccgcg gctgatctat
     1501 gcgtcgctca ccgcgtatgg cgagacgggg gaggaagcgg gtaaaaccgg cttcgacgcc
     1561 acggcctatt gggcgcgctc cgggctcatg gatgaggtcc ggcccgacca tcgggcgatc
     1621 ccagcacgct cggttgccgg aatgggcgac cgtccgaccg ccatggcact atatggcggc
     1681 atcgtcaccg cactctacca gcgggaaaag accggacttg gaggcgaggt gcgcgcctcg
     1741 ctgatggcca atgggatgtg ggccaatggt tacctgatcc aggcgcggct ctgcggcgtc
     1801 gatttcccgc ctcggccacc gcgggaggaa tcagttagcg ccctgggcaa tatttatcgg
     1861 acctcggatg accggtggtt catgctggcg gtgctgaacg aacggcaatg gccggcgctg
     1921 gcgcgcgcgg tcggaatgcc cgaactggcg ctcgacccgc gcttcgcgca accccgcgaa
     1981 cgcggtgtca acgctgtcgt gctgatgggc atcctcgatg aggtttttgc ccgccggccc
     2041 ttggccgaat ggcgaaccat tctcgatgcc gccggcctga ccttcggagt cgtcggcacg
     2101 gtggcggaga tcgcgaccga tccacaggct gtcgctgcgg gaatcctgcg cccactcggc
     2161 gatatcggca tgatgacggt ggacagcccg ttcacgttga gcgcagccga aaaagtgccg
     2221 gttgaagtgg cgccgggtta tggcgagcat agccgcgcga tcctgctggc cgccggctat
     2281 tccgagacgg aagtttccga tctgcgatcg tccgggacga tcgtctaagt aatcaggtca
     2341 gcaatccggg cctcagtcca taatcaagcc gccatcgaca ttgaacgact ggccggtaat
     2401 gttccgggca ccggatgacg ccaggaagac caccatggca gcaatatcct ccgggtcgtt
     2461 tgcgcgtcgc agcgggatga cttcggctcg gcggcgctcc acttcctcga ggctgatccc
     2521 ttcctgttgc gcgcggatgt gcaggttggc atccgacagc gcggtgcggg tgacgccggg
     2581 gcagaccgaa ttgacgttga tgttgtaacg acccagttgt tgtgcggcgg tcttcgtcag
     2641 gctgatcacc gcgcccttgc tggccgcata ggccgcattc gaggtgccca catacccttt
     2701 gccggcgatc gaggcgatgt tgatgattcg gccgctgcgc tgcggaatca tctcccgcgc
     2761 cacgcgctgc aggcagaaga atacgccctt cgcattgacc cgatggatgc ggtcccagtc
     2821 ggcttccgtc aggtccatga tgtcggcccg tctggtgacg ccggcgttgt tgaccaggat
     2881 atcgatctgc ccaaagagcc ccagcgcgcg tcgtaccatg cggtcgatgt cgccgaggtc
     2941 accgacatct gccgagacgg cggcggcacg ttgttgcaac gatatgatgg tctccgccgt
     3001 ctcctcggcc ttgcgtgcgg cgatgtcggc cacgacgaca ctcgcacccg cggcggccag
     3061 ggccagcgcc gcggctttgc cggttccctg tcccgcgccg gtcaccaggg cgacttgatt
     3121 ggtcaagacc acgcgacatt tctccggttc ggccccggcg gcagaaaatg cacctaccct
     3181 cccgcgccgg tgcgggaggg cgtgctccgc gcatcacacc acgctgtcgg cgcgcagctt
     3241 ttcgacttcg gcgtggctca ggccgagcca gttcgtcaga acgtcgtcgg tgtgctgtcc
     3301 cagcatgggc gatgccgata cgcgcggcgg cttgccatcg acccggaccg gccacgccgg
     3361 catcttgaac gggtggtgtt tcggatgctg catcacctgc atgatgccgc gttcctcgaa
     3421 ggtcgggtcg ttctgcaatt ccatcgtgtc gagtaccgca cccgcaggga tgccggcgcc
     3481 gccgacctgt gccatcgcct cgtgcttggt gcgcgttcgc gtccactcgg aaatgatcgc
     3541 atccagcacg gcatcgtgct tcacacgatc ggccggcgtg gcaaagcgtg gatcggtgat
     3601 cagatcctca cgccccatca ccttgcacag ccgcgaccag tgttcagggt tggcgcggct
     3661 ggtcatgatc cagacgtagt cgttcgggcc gcccggggcg cacgcataca gccccgatgg
     3721 cgcattgctg ccaccgccgg accgcgtgcc gtcacgctcc accgctttgc cgctgcgtgc
     3781 ctgagtggag aagttggtgc gcatgtaatg cagcatcgca tcctgcatcg ccacctggag
     3841 ccggtggccc tcgccggtct ttgcccgctt gtgcaacgcg cccaggatgg tgatcgccat
     3901 cgtcatgccc gtgccggtat cgcccagcga caaacccggc ttcaccggct gacggccgcg
     3961 ctcaccggta acgctgatag tgccgccggt agcttgtgcg atcatgtcga aggccaggtt
     4021 ggtctcgtac gggcttccgg tgccgaaacc cttcacctgg caatagatga tgccgggatt
     4081 gagttcgcgc acgacgtcat agccgagacc aagacgctcg atggtgccgg gcgccatgtt
     4141 ctccacgcac acatccgcct tgcgcagcag gtccttcacc aacgcaagcc cctgcggcga
     4201 tttcaggttg accgtgatcg acttcttgtt cgcattgaac atgtggaagt aatacgggtc
     4261 gtcatccggc tggcccggac gcaggcggcg gcctggatcg ccggtcttgg ggttttcaat
     4321 cttcaccacc tcggcgccaa gccatgccag cgtctcggtg caggacgggc cggcttcaaa
     4381 ctgagtaaaa tcgacgacgc ggatacctgc tagaaaatcg agatcctgac tgttgtccga
     4441 catttccgtt ctcctccatc ggtggccgtg gttgctttgc ggcattgcag gaaatcatgg
     4501 cacaacggac tggctcatcc aaggtagccg cggtttggct gtagccacct ccgcggttcg
     4561 gcaggtatcc ccggtccctg tgccagagat ccaatgctgg gcttgaccta caggctttcg
     4621 taggagaacg tccgcctatt ctgcgcaatg ccgtccaaat aattcatgta gaaagcagag
     4681 gagcgaagcc gtggccaata cccaatcaag cggacccgtc gcgccgtccg gcctcaatca
     4741 tttggtcatc aacgtccgga atatggaaga gtcgcaccgg ttctggaccg agatcctggg
     4801 attcacccag gtcgga
//
LOCUS       DASWQN010000807         3931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_79262, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000807 DASWQN010000000
VERSION     DASWQN010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79262"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VGC82_13640"
     CDS             complement(<1..716)
                     /locus_tag="VGC82_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY0424359.1"
                     /translation="MTKPLQGLLVVSLEQAVAAPYCAARLADAGARVIKIERAEGDFA
                     RGYDTAVQGLSSYFVWLNRGKESLVADIKQSEDAALLHRLIAKADVFIQNLAPGAAAR
                     SGFGSETLRARHERLITVDISGYGETGEYAAMKAYDLLVQAESGLATVTGHPAGPGRV
                     GVSIADIGCGMNAYAGVLEALIERSRTGFGKGLKVSLFDAVADWMNVPLLYFDGTGKS
                     PARMGLAHPSICPYGAFATK"
     gene            complement(1112..2212)
                     /locus_tag="VGC82_13645"
     CDS             complement(1112..2212)
                     /locus_tag="VGC82_13645"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEY0424360.1"
                     /translation="MPLTTVSNGRTYDWSYAVGRGAATGTGFNYPQTMCLGNDGDIFV
                     ANRGNENNFGQRVNRIKLGAPGEEELLAEFFQNGQGDGKSTWPFGVAVDSKNNVYVSD
                     EWTNSISVFHGNGTFIRKWGETGSGDGQLLRPAGLACDKDDNVIVVDSGNNRLQVFTG
                     EGKFLSKFGKAGSGDGEFNQPWGITLDSAGDIYVADWKNHRIQKLSPEGQFLMKIGEY
                     GTVPAAADAFAVTYLGPYVSTTPPAEYPKAGLLNHPTDVAVDNDGDIYIADWGNHRIS
                     IFDRHGRPLTNLMGDAQVMSKWGQQSIDANPDMMKALRRVRNLEPQWRFCFPTAVDFN
                     PKTDEIIVADSQRNRLQIYKKVRDYVDFQANL"
     gene            complement(2334..3464)
                     /locus_tag="VGC82_13650"
     CDS             complement(2334..3464)
                     /locus_tag="VGC82_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0424361.1"
                     /translation="MALAQKDPVLVVLQLTGGNDYLNTVIPYNNPLYQDNRKAVGIPE
                     SQILAFDNEYGFPPYLAPLKKIWDENKLAIMHGVGYLDSPRSHFRSMDIWHTCEAEKV
                     GTEGWLGRVVREFDPKKENVVTAVSFGPCLFRALAVPGVPVACVAGSLERYGFLPNIQ
                     EPTQRQQVLDRFSRMYQPIEGSGVMEYLGTTGLDSLKGADILKVAPSLYKSDVKYPNT
                     SIASKLRGIAQVHLANLGTRVFYCDHGSFDSHAGQNPLHAKLWTDVSEGLDAFMTDLR
                     QHDAADNVIVLMFSEFGRRVRDNGSGTDHGAAGATFVLGDRVKGGHYGEYPSLAAEKL
                     VQGDLNPSMDFRGIYSTILDKWLKVDPVPIVNGTYEQPAFLN"
     gene            complement(3490..>3931)
                     /locus_tag="VGC82_13655"
     CDS             complement(3490..>3931)
                     /locus_tag="VGC82_13655"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY0424362.1"
                     /translation="LVGGYELPKPGYGELSMQPSYMGQDLLNPPSVEGWHTGSEWINS
                     GSLMARINFVADQLGNQSLPGVQAIIGRLKAKGTLQPEQLVTDCLDLLGPVEVGADTR
                     KELMDQAKQWGQLSWDSEPNAQMASRRATEMMQLIVATREFQFA"
BASE COUNT          714 a         1241 c         1159 g          817 t
ORIGIN      
        1 tctttcgtgg cgaacgcgcc ataggggcag atcgacgggt gcgccaggcc catccgcgcc
       61 ggcgacttgc cggtgccgtc gaagtacagc aggggcacat tcatccagtc ggcaacggca
      121 tcgaacaggc tgaccttcag gcctttgccg aacccggtcc gggagcgttc gatcagcgct
      181 tccaatacgc cggcgtaagc gttcatcccg cagccgatgt cggcgatcga cacgccgaca
      241 cggccgggac cggcaggatg gccggtaacc gtcgccagcc cggattccgc ctgcactaaa
      301 aggtcgtagg ccttcatcgc cgcgtattcg ccggtttcgc cgtagcccga tatatcgacg
      361 gtgataaggc gttcatgtcg ggcccgcagg gtctccgatc cgaacccgga cctggcggcg
      421 gcgccagggg cgaggttctg gatgaacaca tccgctttgg caatcagccg gtgcagcaac
      481 gcggcgtcct cggattgttt gatgtccgca accaaacttt ctttgccgcg attgagccag
      541 acgaagtagc tggacaagcc ctgcaccgcg gtgtcgtagc cgcgcgcgaa gtcgccctcc
      601 gcccgttcga tcttgatcac ccgcgccccg gcgtcggcca accgagcggc gcaatatggc
      661 gcggctacgg cttgctccaa cgagacaacg agcaggcctt gcaatggttt ggtcatgatc
      721 ccgatccttg tcagctggcg aagccccagg ggcgcgccat accgcatcga caggaccata
      781 cctgcgcacg cggaggcaat accggctcgt ccaccgcata agcaatcggc tgaatcgttc
      841 tatgagcgga caccggtttc gccgtccggt tcgtggatgg cagtagatct caccaccaaa
      901 ttcttaccgt tggcccatac aattcctggc ccatcccacg cacgtctagg tgggtcgaaa
      961 cgaaacgccg cgacgttggg aaacgccgcg gcgcgcgaag gaacgcaagg caggccgacg
     1021 cctatttact cggacgtcgg tctagctcat tgttttagcg agcatcttca cccgttcaga
     1081 taatctatct gaacgggatc tgctctagag gctaaagatt agcctggaag tccacgtaat
     1141 cgcggacctt tttatagatc tgcaagcgat tgcgttgact gtccgcgacg atgatctcat
     1201 cggtcttcgg attgaagtcc accgctgtcg gaaagcagaa gcgccattgc ggttccaggt
     1261 tcctgacgcg gcgcagcgcc ttcatcatgt ccggattggc gtctatgctt tgctgacccc
     1321 atttggacat cacctgggca tcacccatca ggttggtcag cggccgtccg tgcctgtcga
     1381 aaatgctgat ccgatgattg ccccagtcgg cgatgtagat atcgccatca ttgtccacgg
     1441 caacgtcggt gggatgattg agcaagcccg ccttcggata ctccgccggc ggcgtggtcg
     1501 agacataagg tccgagataa gtgaccgcga acgcatccgc tgccgcgggc acggttccat
     1561 attcgccgat cttcatcagg aactggcctt cgggcgacag cttctggata cggtggttct
     1621 tccaatccgc tacatagatg tccccggcgg agtcgagcgt gatgccccag ggctggttga
     1681 actcgccgtc gccgctaccg gccttgccga atttcgacag gaacttgcct tcgccggtga
     1741 acacctgcag gcggttgttg ccactgtcca cgacgatcac gttgtcgtct ttatcgcacg
     1801 ccaggccagc cggacgcagc aactggccat cgccgctgcc ggtctcgccc catttgcgaa
     1861 tgaaggtgcc gttcccgtgg aataccgaga tgctgttcgt ccactcgtcc gaaacgtaca
     1921 cgttgttctt gctgtccacg gcaacgccga acggccacgt ggattttccg tccccctggc
     1981 cgttctggaa gaattccgcc agtagttcct cttcgcccgg cgcgcccagt ttgatgcggt
     2041 tgacccgttg gccgaagtta ttttcgttgc cgcggttggc gacgaagatg tcgccgtcgt
     2101 tgccgagaca catcgtctgc ggatagttga atccagtccc agtcgccgcg ccacgcccaa
     2161 cggcgtagct ccagtcatag gtccgtccgt tcgaaaccgt cgtaagcggc atggcgttct
     2221 cccgtcgcag cttgtggagc cttccgttgg acggaaggct aattacgcac gtccctcgtt
     2281 cctccaggac cggggttccc cgcgccggag gaacgaggct gtagtcggac cgcctagttc
     2341 aggaaggccg gctgctcgta ggtgccgttg acgatcggaa ccggatcgac cttcagccac
     2401 ttgtccagta tcgtcgagta aatgccgcgg aaatccatgc tggggttcag gtcgccctgc
     2461 accagtttct ccgctgccag cgagggatac tcgccgtagt ggccgccctt gacccggtcg
     2521 ccaagaacaa acgtcgcgcc ggccgcgcca tggtcggtgc cggagccatt gtcgcgcacc
     2581 cggcggccga actccgagaa catcagcacg atgacgttgt cggcggcgtc gtgctgacga
     2641 aggtcggtca tgaacgcatc cagcccctcg gacacgtcgg tccacagctt cgcatgcagc
     2701 gggttctgcc cggcgtggct gtcgaagctg ccgtggtcac agtagaagac gcgggtgccc
     2761 agatttgcca gatgcacctg ggcgatgccg cgcagcttgc tggcgatcga cgtgttcgga
     2821 tacttcacgt cggacttgta cagcgacggc gcgaccttca ggatatccgc gcctttcaag
     2881 gaatccaggc cggtcgtgcc aaggtattcc atcaccccgc tgccttcgat cggctgatac
     2941 atgcgggaga aacggtccag tacctgctga cgctgcgtgg gctcctggat attcggcagg
     3001 aagccgtagc gttccagcga gccggcgacg catgccaccg gtacccctgg aaccgccagt
     3061 gcgcggaaca ggcacggacc gaagctcacg gccgttacga cgttttcctt cttcgggtcg
     3121 aactcacgca caacgcggcc cagccagcct tcggtgccga ccttttccgc ctcgcaggta
     3181 tgccagatgt ccatcgagcg gaaatgcgac cgcggtgaat cgaggtaacc cacgccgtgc
     3241 atgatcgcga gtttgttctc gtcccaaatc ttcttcagcg gtgccaggta cggcgggaag
     3301 ccatattcgt tgtcgaacgc gagtatctgg ctttccggta ttccgaccgc cttgcggtta
     3361 tcctggtaca gcggattgtt gtacggtatc accgtattga ggtagtcgtt cccgcccgtc
     3421 agttgcagca ctaccaggac aggatctttt tgcgccaatg ccatttgcat cttcctccta
     3481 tgaatttcgc taggcgaact ggaattcccg cgtcgccacg atcagttgca tcatttcggt
     3541 ggcgcgcctg gacgccatct gagcattcgg ttcgctatcc cagctcaatt ggccccattg
     3601 cttcgcctgg tccatcagtt ccttgcgggt gtcggcaccg acttccaccg ggcccagcag
     3661 gtcgaggcag tcggtgacaa gttgctccgg ttgcagcgtg ccctttgctt tcagacggcc
     3721 gatgatggct tgcacaccgg gcagcgactg attgccgagc tgatcggcga cgaagttgat
     3781 ccgcgccatc agcgagccgc tgttgatcca ctccgacccc gtgtgccagc cctcgacgga
     3841 tggcgggttg agtagatcct ggcccatgta ggacggctgc atcgacagtt cgccatagcc
     3901 aggcttggga agttcatagc cgcccaccag g
//
LOCUS       DASWQN010000808         4493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_474478, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000808 DASWQN010000000
VERSION     DASWQN010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474478"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VGC82_13660"
     CDS             <1..1186
                     /locus_tag="VGC82_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0424363.1"
                     /translation="PRGRDLDLPGRQEAAANIHIGIDWLASVSFGHIDKIGRRVIVLG
                     GGNTAMDCCRSSRRLGGDDVKVIVRSGFEEMKASPWEKQDAIAEDIPILNYLVPKEFK
                     HDRGRLYGMTFEAVKAEYDARGRRKLVPTGEPDVFFECDDVLVAVGQENAFPWIERDI
                     GLSFNEWDMPVVDGITFQSTEPRVFFGGDAALGPKNIIWAVAHGHQAAISIHQFCQGI
                     DVKDRPPPMVTLASQKMGIHEWSYDNGVSLDLRFKVPHRDKQIALKDIKAEVELGFDD
                     KLAFAEAQRCLNCDVQTVFTDKLCIECDACVDICPVDCITFTDNGTEGELRTRLMAPA
                     HNTEQALYVSGALKTGRIMAKDEDVCLHCGLCAERCPTGAWDMQRFLIDVAQAENACC
                     SS"
     gene            1171..3009
                     /locus_tag="VGC82_13665"
     CDS             1171..3009
                     /locus_tag="VGC82_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY0424364.1"
                     /translation="MLQQLDRVEGLQRVNEFVVKFANVNGSGSASANALFAKAVLRMG
                     IVATPRNIFPSNIQGLPTWYEVRISQAGHLAARGGGVDLMVSMNPQTWDQDMQSLEPG
                     GYLFYDNTKPLPAETFRSDIVVLGVPLTEITNRAYRDSRQRQLFKNIVGLGALSALLD
                     MDSAAIEQLLAEQFKGREKLITANIQALNMGRAWVKQNMDWPIGLTLRHADTVGDRIF
                     IDGNSAAALGAVYGGATVCAWYPITPSSSLAEAFTRYCGMTRVDKVTGKNNFAIVQAE
                     DELSSIGMVIGAAWNGARAFTATSGPGISLMQEFLGLAYFAEIPAVIFDVQRAGPSTG
                     MPTRTQQTDIMACAYASHGDTKHVLLLPDDPFECFEFAALAFDLADRLQTPIFVMLDL
                     EIGMNERLSKPFQWDDSRRMDRGKVMTAEQLEAGKQFGRYLDVDGDGIAYRTLPGTHP
                     SKGAFFTRGTSKDRMARYSEEGHDYLENMERLLRKHVTARSLVPAAIERRAFVPTRRG
                     AIYYGSTSPAIDEANLLLADKGIYIDLLRVRGFPFGQEVETFIAAHDHVFVVEQNRDG
                     QLRSLLVNELEIDPKKLGKVVHYDGTPISAGFIAGAIAGRIESGVL"
     gene            3006..3848
                     /locus_tag="VGC82_13670"
     CDS             3006..3848
                     /locus_tag="VGC82_13670"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY0424365.1"
                     /translation="MTFLAKPKLHHPELHKNSLGYTRRDYEGAISTLCAGCGHDSISA
                     AIVRACFELELPPHRIAKLSGIGCSSKTPTYFLGASHGFNSVHGRMPSVLTGANLANR
                     DLIYLGVSGDGDSASIGLGQFAHSMRRGVNMVYIVENNGVYGLTKGQFSATSDRGAKS
                     KRGVVNSDEAIDLVELALQLGATFVARSFSGDKAQLVPLIEAALKHEGAAFIDCISPC
                     VAFNNHDGSTKSFDYVREHNAAVNYLDVIPGAAGDRRRLCSRGDRGGHSARRLDTAAK
                     KATC"
BASE COUNT          919 a         1230 c         1397 g          947 t
ORIGIN      
        1 gccccgaggg cgcgacttgg atctgcccgg tcggcaggaa gcggcagcaa atatccatat
       61 cgggatcgat tggctggcct cggtttcatt tggtcatatc gacaagatag gccgacgcgt
      121 gatcgtgctg ggtggcggca acactgcaat ggattgttgc cggtcctctc gccgcctggg
      181 tggcgacgac gtcaaggtca tcgtccgttc ggggttcgag gagatgaagg cctccccctg
      241 ggaaaagcag gacgcgatcg ccgaggacat ccccatcctg aactacctgg tgccgaagga
      301 attcaagcac gacagaggcc ggctgtacgg gatgactttc gaggcggtga aggccgaata
      361 cgacgctcgc gggcgtcgta aattggttcc caccggggaa cccgacgtct tcttcgagtg
      421 cgatgacgtt ctggtcgcgg ttggtcagga gaatgcgttt ccctggattg aacgcgatat
      481 cggcctgagc ttcaacgaat gggacatgcc ggtcgtggat ggaatcacct ttcaatccac
      541 cgaaccgcgt gtgttcttcg gcggcgatgc ggcattgggc cccaaaaata taatttgggc
      601 ggtcgcacac gggcatcagg cggcgatctc gatccaccag ttctgccagg gcatcgacgt
      661 caaggatcga ccgccgccca tggtgacgct agccagccag aaaatgggca ttcatgaatg
      721 gagctatgat aacggcgtct cgcttgacct gcggttcaag gttccgcatc gcgacaagca
      781 gatcgcattg aaggacatca aggcggaggt cgaactgggt ttcgatgaca agttggcgtt
      841 cgccgaagca caacggtgcc tgaactgtga tgtgcagacc gtcttcaccg acaaactatg
      901 catcgaatgc gacgcctgtg tggatatctg cccggtcgac tgcatcacct ttaccgacaa
      961 tggaactgaa ggcgagctgc ggacgcgtct gatggccccc gcccataata cggagcaggc
     1021 gctgtacgta tccggtgcgt tgaagactgg gcgaatcatg gcgaaggacg aagatgtctg
     1081 cctccattgc ggactgtgtg cggagcgttg ccctacgggc gcgtgggata tgcaacgttt
     1141 cttgatcgat gtggctcagg cggagaacgc atgctgcagc agctagaccg tgtagaaggc
     1201 ctccaacggg tcaatgaatt cgtcgtcaaa ttcgccaatg tgaatggctc gggatcggcc
     1261 agcgccaatg ctctgttcgc taaggccgta cttcgcatgg gcatcgtggc gacgcccagg
     1321 aacatctttc cgtccaacat acaagggtta ccgacatggt atgaggtacg gatctcccag
     1381 gccggccatc tggcggcacg tggcggcggc gttgacctta tggtctcgat gaatccgcag
     1441 acctgggacc aggacatgca gtcgctggaa ccaggcgggt atctttttta cgacaatacc
     1501 aaaccgctgc cggcggaaac atttcgatcg gacatcgtgg tcctcggtgt accgctgacc
     1561 gaaatcacaa acagggcata tcgtgattct cgccagcggc aattgttcaa gaacattgta
     1621 ggcttgggcg ccctgtcggc gctgctcgat atggattcgg ccgccatcga gcagttgttg
     1681 gcggaacaat tcaagggacg cgaaaaactg atcacagcca atattcaggc gctgaatatg
     1741 ggccgtgcgt gggtcaagca gaacatggac tggccgatcg gcctgacgct tcggcatgct
     1801 gacacggtgg gcgaccgcat ctttatcgat ggaaatagtg ccgccgcgct cggcgcggtc
     1861 tatggcggtg caacagtctg cgcttggtat ccgatcaccc cgtcttcgtc gctggccgag
     1921 gccttcaccc gctattgcgg catgacgcgg gtggataagg tcaccgggaa gaacaacttt
     1981 gccatcgttc aggctgaaga cgaattgtcg tctatcggca tggtgatcgg tgctgcctgg
     2041 aacggcgccc gagccttcac ggctacgtca ggacccggca tttcgctgat gcaggaattc
     2101 ctcgggctgg cgtatttcgc cgaaattcct gcggtcatct tcgacgtgca acgcgctgga
     2161 ccttctaccg gcatgcccac gcggacgcag cagactgaca tcatggcctg tgcttatgct
     2221 tcgcacggcg acaccaagca tgttctgctg ctgccggatg accctttcga gtgcttcgag
     2281 ttcgcggctc tggccttcga tctggcagat cgtctgcaaa cgccgatttt cgtcatgctc
     2341 gacctggaaa tcggcatgaa cgaacggttg tccaagccct tccagtggga cgatagccgc
     2401 cggatggacc gtggcaaggt gatgacggcg gagcaactcg aagccggaaa gcagttcggc
     2461 cgttatctgg atgtcgacgg cgatggcatt gcctatcgta ccctgcctgg cacgcacccg
     2521 tcgaaaggcg cgttctttac ccgtggcacg tccaaggacc gtatggcgcg ctactcagaa
     2581 gaaggccacg actatctgga gaacatggag cgactgctgc gcaagcacgt cactgcccgc
     2641 agtctggtgc ctgcggcaat tgagcggcgg gcatttgtcc cgacgcgacg gggggccatc
     2701 tattacggct ccaccagccc cgccattgac gaagccaacc tgctattggc ggacaaggga
     2761 atttacatcg acttgctgcg ggtgcgggga tttccattcg gccaggaggt tgagaccttc
     2821 atcgcggcac atgaccacgt gtttgtcgtg gagcagaacc gtgatggcca gttacgatcg
     2881 ttgctggtaa atgaactgga aatcgatcca aagaaactgg gcaaggtggt gcattacgac
     2941 ggtaccccca tcagtgcggg ctttatcgcc ggggcgattg ccggccgcat cgaatcgggt
     3001 gtcctatgac ttttctcgcc aagccgaagc tgcatcatcc cgaactccat aagaatagtc
     3061 ttggatacac gcggcgcgac tatgaagggg cgatctcgac cctttgcgcc ggttgcgggc
     3121 acgattcgat tagcgcggca atcgtccggg catgttttga gcttgaactg ccaccgcacc
     3181 gaatcgccaa gctttcagga atcggctgtt cgtcaaagac cccgacctat ttcttgggag
     3241 cgtcgcacgg cttcaactct gtccacgggc ggatgccatc ggtgctgacc ggggctaatc
     3301 tcgcgaaccg cgatctgatc tatctcggtg tgtccggtga cggcgacagc gcctcaatcg
     3361 ggctggggca gttcgcgcat tcgatgcggc gcggcgtgaa catggtctat attgtcgaaa
     3421 ataacggcgt gtacggactg accaagggcc agttctcggc gacttccgac agaggtgcca
     3481 agtcaaagcg tggtgtcgtc aacagcgatg aggcgatcga cctcgtcgaa ctggcgcttc
     3541 agcttggagc gacattcgtc gcgcgcagtt tctcgggcga caaggcgcag ttggtgccgt
     3601 tgatcgaggc cgcgctgaag cacgaaggtg cggccttcat cgactgcatt tccccgtgtg
     3661 tggcgttcaa caaccatgat ggatcgacca aaagctttga ttacgtacgc gagcacaatg
     3721 cggcggtgaa ttatttggat gtgataccag gggcggcagg agatcgccgt agattatgct
     3781 ccaggggcga tcgaggtggt cactcagcac gacggctcga cactgcagct aagaaagcta
     3841 catgctgatt acgaaatgca tgatcgcgtt tcggcaatga cctacatgca gcagcgacac
     3901 gcggctggcg agattgtaac cggattgctc tatgttgatc cgaaccctcg agacctgcac
     3961 ggtcaccttg cgacgtcagc tacgcctttg aatgccatgc aggaagaagc gttggttccg
     4021 ggatcgtcgg tactagcgaa gatcaatgcg gggcttcggt aggagtggct ggaatcgaac
     4081 cgcggcccgg agcattccat ctgctggcaa cggcaggcat cggcagatcg cttcccgccc
     4141 ggtccgaccg ccgataagtc ctgctagacc caataccgtt gaattaagtg gcctgcttgc
     4201 atttcggcgg ggcctttgca gcggcggaat aggccgctga tttgccaggc tggcgttgtc
     4261 tatgtccagc tctttgggat ggtgatgtat gggctgggac gagcagctgg atgtgatcgt
     4321 catagtggat gcgttggatt gatgatggcg cggcccgcgc tttggtgggg aagttgctca
     4381 tcttgcgttt gacagcgcgc ggattgtgtc gcccgcggct gcgttcggcc ggtagcgaga
     4441 tgatctccag gagcacgaga tcaaccatcc ggcgtcgctg ccgaggggga aaa
//
LOCUS       DASWQN010000809         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2586867, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000809 DASWQN010000000
VERSION     DASWQN010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2586867"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /gene="der"
                     /locus_tag="VGC82_13675"
     CDS             complement(<1..783)
                     /gene="der"
                     /locus_tag="VGC82_13675"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY0424366.1"
                     /translation="MDSKHAGESIGTVAIVGRPNVGKSALFNRLVGRNIAIVHDQLGI
                     TRDRLAALSSKGRRPFTVWDTGGIGGAGETELTTQVRGAADSAMRESDVILFIVDAQA
                     GLNPIDEELAKLLRKSKRPVVLVVNKIDHEKHEDLDADFARLGFDNSIPISAAHGRGI
                     SDLLEVVDGLLPETQAPNPKSEIESAATSLAIVGRPNAGKSSLINSILRDARTIVSEL
                     PGTTRDAVDILYERSGEKFLLIDTAGMRQRSKHSTSVEVFSVM"
     gene            complement(796..1419)
                     /gene="pgsA"
                     /locus_tag="VGC82_13680"
     CDS             complement(796..1419)
                     /gene="pgsA"
                     /locus_tag="VGC82_13680"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY0424367.1"
                     /translation="MMGRPLMTTANKITVIRILMIPVFVTLAIYYGESIQEGNPQDWM
                     RFTAITVFLLAAISDGLDGYVARRYNQRSSLGVILDPIADKGLLLSGIITLSISNWSQ
                     VDPHYGKFPAWFPVLVITRDLVIVSGSLLLHVLNGKVRVRPSWTGKVATVLQMAAIAW
                     VMLQMRILPLIYIVFAAGFFTLVSGVIYFRNGLRQLSAEGHANAKLG"
     gene            complement(1467..>1801)
                     /locus_tag="VGC82_13685"
     CDS             complement(1467..>1801)
                     /locus_tag="VGC82_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY0424368.1"
                     /translation="DALRSNEEALEFVGHFFREGKPVAAICHAAWVLIDAEVVRGRTL
                     TSWPAIKTDIRNAGGNWVDQEVVVDSGLVTSRKPDDIPAFNEKMIEEFCEGRHSATSG
                     KHPGELET"
BASE COUNT          359 a          564 c          522 g          356 t
ORIGIN      
        1 catgacgctg aagacttcca cggaagtcga atgcttactc cgttggcgca ttccagctgt
       61 gtcgatgagg aggaattttt ccccgctgcg ctcataaaga atatcgacgg catcccgggt
      121 cgtgcccggc aattcgctga cgatggtgcg cgcatcgcgg aggatcgaat tgatcagcga
      181 ggactttccg gcattcgggc ggccgacgat ggcgagggaa gtggcggcac tctcgatttc
      241 ggattttgga tttggcgcct gggtttcggg aaggaggcca tccacaactt cgaggagatc
      301 agaaatgccg cggccgtgcg cggcgcttat cgggatggaa ttatcgaaac ccagccgcgc
      361 gaaatccgca tccagatcct cgtgcttctc gtgatcgatc ttgttcacca ccaggacgac
      421 agggcgcttc gatttgcgca gaagtttcgc cagctcctca tcgatcggat tcagacccgc
      481 ctgcgcatcg acgatgaaga gaatgacgtc gctctcgcgc atggccgaat cggctgcgcc
      541 ccgcacctgg gtggtcaact ccgtctcgcc cgcaccgccg atgccgcccg tatcccagac
      601 cgtgaacggc cggcggccct tgctcgacaa ggcggccagg cgatcacgcg ttatgcccag
      661 ctgatcgtgg acaatggcga tgttccggcc gaccagccga ttgaagagcg cggatttccc
      721 cacgttcggc cgaccgacaa tagcgacggt gccgatactt tccccggcgt gtttcgaatc
      781 catgacgctg gcactctagc ccagctttgc gttggcgtgg ccttctgccg ataattgccg
      841 caatccgttc cggaaataaa taacgccgga taccagggtg aagaacccgg cggcgaagac
      901 gatgtagatc aacggcagga tccgcatctg cagcataacc caagcgatcg ccgccatctg
      961 cagcaccgtc gccacctttc cggtccagct cggacgcacc cgcaccttcc catttaaaac
     1021 atggagcagg aggctgccgc tcacgatcac gaggtcgcgg gtaatgacaa gcacggggaa
     1081 ccaggccggg aatttgccgt aatgcggatc gacctggctc cagttgctaa tgctgagcgt
     1141 gatgattccg ctgaggagca ggcccttgtc cgcaatcgga tccaggatca ccccgagcga
     1201 gctgcgctgg ttgtagcggc gcgccacgta cccgtcgaga ccgtcactga tcgccgccag
     1261 caaaaagacc gtgatcgccg tgaagcgcat ccagtcctgc ggattccctt cctggatgct
     1321 ttcgccgtaa tagatcgcga gggtcacaaa caccgggatc attaggatcc ggatcacggt
     1381 gatcttatta gcggtggtca tgagcgggcg gcccatcatg aaaacttaga cggcctcgcc
     1441 gccccagatg caaggcaatt tgcccgctag gtttccagct cgcccgggtg cttcccggac
     1501 gtcgcggagt gccggccttc gcaaaattct tcgatcatct tttcgttgaa ggccgggatg
     1561 tcgtccggtt tgcggctcgt gaccagcccg ctgtcgacga cgacctcctg atccacccag
     1621 ttcccgccgg cattgcgaat atcggtcttg atggccggcc atgaagtcag ggtccgtccg
     1681 cgcaccactt cagcgtcgat caacacccag gccgcatggc agattgccgc caccggtttt
     1741 ccctcgcgaa aaaaatggcc gacgaactcg agcgcttctt cgttcgaccg caacgcatcc
     1801 g
//
LOCUS       DASWQN010000810         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_737695, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000810 DASWQN010000000
VERSION     DASWQN010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737695"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VGC82_13690"
     CDS             complement(<1..292)
                     /locus_tag="VGC82_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424369.1"
                     /translation="MFDTDNDGTLDLAEVKKAASDLFARLDRDHDGTLDKRELAGKLS
                     ARELAAADPDHDGTLTAEEYLAVVEQRFNAANPDKDGTLDAKELNTSAGRVLL"
     gene            1516..>1866
                     /locus_tag="VGC82_13695"
     CDS             1516..>1866
                     /locus_tag="VGC82_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424370.1"
                     /translation="MRKIHGVGPGFLACLTSTLLIALVCPEALADQLSITPASLARIG
                     TVDERFQSYNVEMAEVTGGRFWKPYGPNTSDGHSDLYEYRTPTDLTNPRLRRLAAALA
                     PAYMRVSGTWANATY"
BASE COUNT          338 a          554 c          546 g          428 t
ORIGIN      
        1 gcaggagcac cctcccggcg ctggtattta attccttggc atccaaggtt ccatccttat
       61 ccgggtttgc cgcgttgaag cgttgctcga cgaccgccag atattcctct gccgtcagcg
      121 ttccatcgtg atcagggtcg gcagcggcca gctccctggc actcaatttg cctgccaatt
      181 cgcgcttgtc gagagtgccg tcgtgatcgc ggtccagtct ggcgaacaag tctgacgccg
      241 ccttcttcac ctccgcaagg tcaagcgttc catcgttatc ggtgtcgaac attcggatcg
      301 gattggggtg gcggggaccc gccaagaccg gcaagcttcc agcgaacaat gtcgcaatcg
      361 tcaacagaat tgaccggcga gatatcatga cagatctctc catccacttc tcctgaactg
      421 gagtccggcg cgaactgcgc gttcccttgc cattgccgcg ctatcgcagc tgacaagtta
      481 tggtcggccg caatagtgtc aatgtgaaag gaactgtcgc ctcgaacttc ccgatctggg
      541 ctgaggcggg ccatgtcgcc gacctccacc taaattagct caacacgcat aacgggtttg
      601 tggtcatacg gtcccgattt ggatataatc gcagacgcga agtgagggtg accgatggga
      661 agtcacgttt acaacgtcgt caaggctccg attgggtggt cgcttttttg cgaccgcgaa
      721 cgcatcggcg ggtacgtttc gagtgaagcg gcgctggagg cggcaacggc gttcggcgca
      781 aaggctctcc gggagggtta cagtattcag atcaacgtgc cgggaccgca actgcatgca
      841 gttgacgact tcgtatcgtg gccggcgcgg tggcacgttg atccaaagta gcgaaccgac
      901 tctccgtcgt tatcgccgac aaacgggccg ctagatcgcg caccgaaccg aggcgtgggt
      961 tttcacaggc gcctttcaac agtcattatc agccgtctta cccgtcggtt tggccccttc
     1021 agcttgctgc gccgccgtgg gttggccctg catctgtttc tgcgcatcct gcgaagacgc
     1081 cgcgacatct ccagttgcgc ggttcatcgt gccggtcggc gggtgctgac tggagtctgt
     1141 actcgtgcct gccgtcgtct gtccggcgct acctacggtg ggcccggaac ctgcgtcctt
     1201 cgcggctttg ccggcggtgt tgcacgggcc ggcctgtgct gtgccggcgc tgagagccac
     1261 aatcgccacg cccgcgagaa tcagtgattt tatgttcatc tgcttctcct tggtttttcc
     1321 ctggcgtgcg cccttgccat accaggagga ggagaaacgg actcgtgcgc gacaggttcc
     1381 gcacggcaaa agcaaatctc gctatggttt tatcgactgg cttcttgttc gcgtggcatc
     1441 aagctcgcta ccttgcgtct gctcaaggca gaccgcttga tccgccggcg tcaagcgccg
     1501 aggcggagaa tttgcgtgcg aaaaattcac ggggtcggtc ctggattcct tgcttgcctg
     1561 acgtcgacgt tgctcatcgc acttgtctgc cccgaggctc tcgccgatca gttgtccatc
     1621 acacctgcga gcttggctcg cataggcacg gtcgacgaac gtttccaatc ctataacgtt
     1681 gagatggccg aggtgaccgg cggaagattt tggaagcctt atggacccaa cacttcggat
     1741 ggacattcgg atttgtacga ataccgtacg ccgaccgatc tgactaaccc gcggttgcgg
     1801 aggctggctg cagcattggc gcctgcttat atgcgtgtca gcggaacctg ggcaaacgcc
     1861 acctat
//
LOCUS       DASWQN010000811         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_343450, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000811 DASWQN010000000
VERSION     DASWQN010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343450"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VGC82_13700"
     CDS             complement(<1..198)
                     /locus_tag="VGC82_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424371.1"
                     /translation="MISDALCWTVAALAALPAVMWLLNLPLFRRPKRPDVCYSVSVIV
                     PARNEEAAIGACLRSLLASTGA"
     gene            complement(195..1043)
                     /locus_tag="VGC82_13705"
     CDS             complement(195..1043)
                     /locus_tag="VGC82_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0424372.1"
                     /translation="MSGNRRSAGLADEAALVLAARSSWITWFMTAYMRRYARRHFHAV
                     RIAGSLPPALDPARGVVVYSNHPSWWDPVMFVLLQAHVFPDRTGFGPMDANALGRYGV
                     LERIGVFGLPPGTRRGAARFLRVSEGVLRTPNTVLWVTAEGGFTDPRQRPVRLRPGVA
                     HLLRHLPDVTAVPLALEFPFWNERTPEALARFGEPIMADAGRDAAAWEALLAERLTDA
                     MDRLAEDVMTRDPDRFQTLVAGKAGIGGVYDVWRRGRAAIRGRRARLDHEGRASKGSP
                     VEQPAP"
     gene            complement(1046..>1871)
                     /locus_tag="VGC82_13710"
     CDS             complement(1046..>1871)
                     /locus_tag="VGC82_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HEY0424373.1"
                     /translation="ADGLVALGETFGASYEPGRGIARIETAQGRAVAVIAEDGERIPV
                     SAVVSNMDAVRTYRELIGGPPAAAFNKARRYEPACSGVVLYLGLNKRYDHLLHHDFVF
                     SRDPEQEFDAIYRRGEPAADPTCYLAAPARTDPHVAPPGGEALYVLVHTPYLRPGQDW
                     REMLPGYRRVILDKLMRTAGLHDLESRIVFEQALTPQDIHDRYRVLNGAIYGLASHGR
                     FFGAFKPGNRSRHVGGLYLAGGAAHPGPGMPMVMMSGWIAADSLDQDVRSNAQAMA"
BASE COUNT          365 a          671 c          571 g          264 t
ORIGIN      
        1 ggccccagtg ctcgcgagca gactccgcag gcacgcgccg atcgcagctt cctcattgcg
       61 tgccggcaca ataaccgaaa cggaatagca gacgtccggt cttttgggcc ggcggaacag
      121 cggaaggttg agcaaccaca tgaccgccgg cagtgccgcc aacgcggcga ccgtccagca
      181 caaggcgtca ctgatcacgg cgccggctgc tccaccggcg agcctttgga ggcacgaccc
      241 tcgtggtcca acctggcgcg gcggccccga atcgcggccc gaccacggcg ccagacgtca
      301 tagacgccgc cgatacccgc tttgcccgcg accaatgttt ggaacctgtc ggggtcgcgt
      361 gtcatcacgt cctccgccaa ccgatccatc gcatcggtca agcgctcagc cagcagcgcc
      421 tcccacgccg ccgcgtcgcg cccagcgtcc gccatgatgg gttcaccaaa acgggccagg
      481 gcctccggcg tccgctcgtt ccagaacgga aactccagcg cgagcgggac ggccgtcaca
      541 tcaggcagat gacgcagcag atgagccacg cccggccgca gccggaccgg acgctgccga
      601 ggatcggtga atcccccctc ggcggtgacc cacaacacgg tattcggcgt ccgcaaaaca
      661 ccttcggaca cgcggagaaa tcgagcggca cctcgacgcg tgcccggcgg caggccgaac
      721 actccgatac gctccagaac gccgtaacgc cccagcgcat tcgcatccat cggcccgaaa
      781 ccggttcggt cgggaaaaac atgcgcctgc aacagcacga acatcacagg gtcccaccag
      841 gaggggtggt tcgaatacac caccaccccg cgtgcgggat ccaatgccgg cggcaggcta
      901 ccggcgatcc gaacggcatg gaaatgccgt ctcgcgtagc gccgcatata cgccgtcatg
      961 aaccacgtaa tccacgaaga gcgtgccgcg agcaccaggg ccgcctcgtc agccaaccct
     1021 gccgaacgcc ggttgcctga catcgtcagg ccatcgcctg cgcgttcgac cggacatcct
     1081 gatccagcga gtcggccgcg atccatccgg acatcatgac catcggcata cccggtcccg
     1141 gatgggcggc gccgccggcc agatacagcc cgccaacgtg acggctacga tttccgggct
     1201 tgaaggcgcc aaagaaccga ccatggctgg cgagcccgta gatcgcaccg ttcagcaccc
     1261 ggtatcggtc gtggatgtcc tgcggggtca gcgcctgctc gaacacgatc cgcgactcca
     1321 ggtcgtgcag gcccgcggtg cgcatcagct tgtccagaat gacgcggcga tagcccggca
     1381 acatttcccg ccagtcctgc cccggccgca gatagggcgt gtggaccaac acgtaaagcg
     1441 cctcgccacc gggcggtgcc acatgcggat cggtccgcgc cggcgccgcg agatagcaag
     1501 tcgggtcggc tgccggctcg ccccggcgat agatggcgtc aaattcctgc tccggatcac
     1561 gcgagaaaac gaaatcatga tgcagcagat gatcgtagcg cttgttcagc ccgagataca
     1621 gaaccacgcc ggagcaggcc ggttcgtacc gccgagcctt gttgaacgcg gcggccggcg
     1681 gtccgccgat caactcgcgg taagtacgaa cagcgtccat gttcgatacc actgcggaaa
     1741 ccgggatgcg ttcgccgtcc tcggcgatga ccgcgaccgc gcggccttgc gccgtctcga
     1801 tcctggcgat cccccggccg ggctcgtagc tggcgccgaa cgtttcgcct aaagcgacca
     1861 atccgtcggc g
//
LOCUS       DASWQN010000812         3987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2321979, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000812 DASWQN010000000
VERSION     DASWQN010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321979"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..1062)
                     /locus_tag="VGC82_13715"
     CDS             complement(304..1062)
                     /locus_tag="VGC82_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEY0424374.1"
                     /translation="MRIEYLYRYPVKGLTAEALEQAEVKAGGAIPWDRAFALAHGDAG
                     FDPANPTWLPKWNFLCQMKHAKAALLFSVFDPGSKTLRIRAPDGSGIEANAMTNAGRE
                     RIGAFLADYMAEEARGQPEFHYVEGHVFGDQKTPVISLNNLASLRDFEAKVGARRHRR
                     RFRANIWFSGAPAWAEREWIGREIQVGGAVLRVLKGTTRCPATEVNPETGERDAHPVE
                     ELRSLYGHVELGVHAEVVEGGQFSVGDAIELMEI"
     gene            1177..3756
                     /gene="clpB"
                     /locus_tag="VGC82_13720"
     CDS             1177..3756
                     /gene="clpB"
                     /locus_tag="VGC82_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY0424375.1"
                     /translation="MDIQKFTERCQGFLQAASTIAIREFHQRITAEHLLKALLDDEEG
                     AAAGLIKAAGGDPKAAAAANDAEVARQPKVSGAGAGQPQLTPELVRVLDSAQQTSTRA
                     GDEYVAQDRLLVALAESNTPAGRALKSGGITPQALEKTVNDLRKGRKVTSPNAESNFD
                     ALKKYARDVTAVAREGKLDPVIGRDEEIRRTIQVLARRTKNNPVLIGEPGVGKTAIVE
                     GLALRIVNGDVPEALKDKQLMSLDLGAMVAGSKYRGEFEERLKAVLKEIEDAQGQVIL
                     FIDEMHTLIGAGRADGAMDASNLIKPELARGTLHCIGATTLTEYRKHVEKDAALARRF
                     QPVFVGEPTVEDTISILRGIKEKYELHHGVRIADAALVAAATLSKRYITDRFLPDKAI
                     DLIDEASSRLRMQVDSKPEALDELDRRVIQLKIEREALKKESDPASKERLVRLEKELA
                     DLEDRSNAMSTAWKNEKDQVQETQKLKERLDQARSEVEVAQRRGDLGRASELMYGVIP
                     DIERQLARSSDGKLVNEAVTEESIAAVVSRWTGVPVDKMLEGERAKLLRMEDSLRRRV
                     IGQEEALEAVSHAVRRARAGLQDPNRPIGSFLFLGPTGVGKTELTKALAAFLFDDDKA
                     MVRIDMSEFMEKHAVSRLIGAPPGYVGYDEGGVLTEAVRRRPYQVILFDEVEKAHEDV
                     FNILLQVLDDGRLTDGQGRTVDFKNTIIVLTSNLGSDVLAQQKEGEPTAMVQGDVMKV
                     VRQHFRPEFLNRLDEIVLFRRLQRSDMASIVEIQLAGLRKLLADRKIGLEPDRSALEW
                     LAAEGYDSTYGARPLKRVIQRSLQNTLAGLILEGEVKEGETVRVSAGASGLVINGQLA
                     EAA"
BASE COUNT          803 a         1188 c         1321 g          675 t
ORIGIN      
        1 aacgccgcgg catacggcct tgacgccatg atcagactga cttgatcgcg ggtaaccgga
       61 agcgacggtt cgtcatcaga ataactgaaa atgtcgcgca tttgggcatc cgaaaattat
      121 ttgccatcct cttgggatga acgtctctcg gtgaaaagag cgccgcggga gcgaaccccc
      181 gcggcaaagt tcaagggagg aaacaccaac cttgtaactc acttttccgc gaaaatgcaa
      241 tcgtattctc actaaaccgt gatcacgttt tcaggtcgat accgaaatat aacttggggg
      301 cgtctatatc tccatcagct cgattgcgtc gccgaccgag aattggccgc cttcgacgac
      361 ttcggcatga accccgagtt cgacatggcc ataaagcgag cggagttcct cgaccgggtg
      421 cgcatcgcgt tccccggtct ctggattgac ttcggtcgca ggacagcggg tcgtgccctt
      481 cagtacgcgc agcacggctc ctcccacctg tatctctcgc ccgatccatt cgcgttcggc
      541 ccaggccggg gcgccgctaa accaaatatt ggcgcgaaag cggcggcggt gtcggcgggc
      601 gcccaccttt gcctcaaagt cccgcaggct ggcgaggttg ttcaggctga tcactggggt
      661 cttctggtca ccgaacacat ggccctcgac atagtggaat tccggctggc cgcgcgcttc
      721 ctcggccatg tagtccgcca gaaaggcgcc gatccgctcg cgcccagcat tggtcatcgc
      781 gtttgcttcg attccgctcc cgtccggcgc gcgaatgcgc agcgtcttgg agccaggatc
      841 gaacacgctg aacagcaagg ctgccttcgc gtgcttcatt tggcaaagga agttccattt
      901 cggcagccat gtcggattgg cgggatcgaa gcccgcgtcg ccatgcgcca gggcgaaggc
      961 gcggtcccac gggatggccc ccccggcctt gacctcggcc tgctccagcg cttcggcggt
     1021 caaccctttg accggatagc ggtacaggta ttcgattcgc atcgtcaaac ccccttgagc
     1081 gtccgtccgc gccgccccac tttgacatca gagttcgctc atgtcgcctt cttacagggg
     1141 catgaacggg ccgtcgcctg atgaaaggga catgagatgg atattcagaa attcaccgaa
     1201 cgctgccaag ggtttctcca ggccgccagc acgatagcga tacgcgaatt ccaccagcgc
     1261 atcaccgccg aacatctgct gaaagcgctg ctggatgacg aggaaggagc ggccgccggc
     1321 ctgatcaagg ccgccggagg cgatccgaaa gccgctgcgg cggccaacga tgcggaagtg
     1381 gcgcgtcagc ccaaggttag tggcgccggg gcgggccagc cgcaactgac gccggaactg
     1441 gtccgcgtcc tcgattcggc acagcagacc tcaacccgcg ccggagacga atatgtggcg
     1501 caggatcgcc tgctggtggc gcttgcggaa tccaatacgc cggccggccg cgctttgaag
     1561 tcaggcggga ttacgcctca ggcgctggag aaaacggtca atgacctgcg caagggacgc
     1621 aaggttacca gcccaaacgc ggagtcgaac ttcgacgcat tgaagaagta tgcccgtgac
     1681 gtgaccgcgg ttgcacgcga aggcaagttg gacccggtca tcggacgcga cgaggaaatc
     1741 cgccgcacga tccaggtcct tgcccgccgg acgaagaaca acccggtgct gatcggcgaa
     1801 cccggcgttg gcaagacggc cattgtcgag gggctcgcgc tgcgcatcgt caacggcgat
     1861 gtgccggagg cgctgaaaga caagcagctt atgtcgctcg accttggtgc catggtcgcc
     1921 ggatcgaaat accgcggcga attcgaggag cggctgaagg cggtcctgaa ggagatcgag
     1981 gacgcgcagg gccaggtcat cctgttcatc gacgagatgc atacgttgat cggcgcgggc
     2041 cgagcggatg gggcgatgga tgcctcgaac ctgatcaagc cggaactggc gcgcgggaca
     2101 ctgcactgca tcggcgcgac cacgctgacc gaataccgca agcacgtaga gaaggacgcg
     2161 gccttggctc ggcgattcca gccggtgttc gtgggcgagc cgacggtcga ggacacgatc
     2221 agcatcctgc gcgggatcaa ggaaaagtac gagctgcacc acggcgtgcg gatcgccgat
     2281 gcggccctgg tcgcggcggc cacattgtcg aagcgctata tcaccgaccg gttcctgccg
     2341 gacaaggcga tcgacctgat cgacgaagca tccagccgtc tgcgtatgca ggtggactcg
     2401 aaaccggagg cgctggatga acttgatcgg cgggtgatcc agctcaagat cgagcgggaa
     2461 gcgctgaaga aggaaagcga tccggcctcg aaggagcggc tggtcaggtt ggaaaaggaa
     2521 ctggccgacc tggaagatcg gtcgaacgcc atgagcaccg cctggaagaa cgagaaggac
     2581 caggtgcagg aaacccagaa gctgaaagag cggctggatc aggctcgctc cgaggtcgag
     2641 gtggcgcagc ggcgcggcga tctggggcgc gcgtcagagc tgatgtacgg cgtcatcccg
     2701 gacatcgagc ggcaattggc gcggtcgtcc gacggcaaat tggtgaacga ggcagtcacc
     2761 gaggaaagca tcgccgctgt ggtgtcccgt tggaccggcg tgccggtgga caaaatgctg
     2821 gagggcgagc gcgccaagct gctgcgaatg gaagactcgc tacgccggcg ggtgatcggc
     2881 caggaggagg ctctggaggc ggtgtcgcac gcggtgcggc gcgctagggc cgggttgcag
     2941 gatccgaatc ggccgatcgg cagctttttg tttcttggtc cgaccggcgt gggtaaaacc
     3001 gaactgacca aggcgctggc ggcgttcctg ttcgacgacg acaaggcaat ggtgcgcatc
     3061 gacatgagtg agttcatgga gaagcacgcc gtgtccaggc tgatcggtgc tcccccgggc
     3121 tatgtcggtt atgacgaggg cggtgtgctg accgaggcgg tgcgtcgccg gccataccag
     3181 gtgatcctgt tcgacgaggt cgagaaggca catgaggatg tgttcaacat cctgctgcag
     3241 gttctggacg acggcaggct gacggatgga cagggacgga ccgtcgattt caagaacacg
     3301 atcatcgtcc tgacgtcgaa ccttggcagc gacgtgctgg cgcagcagaa ggaaggcgag
     3361 ccgaccgcga tggtacaagg cgacgtcatg aaggtcgtgc gccagcactt ccgtccggag
     3421 ttcctcaacc ggctggatga gatcgtgctg ttccggcggt tgcagcgcag cgacatggcc
     3481 tcgatcgtgg agatccagct cgctggcctg cggaaattgc tggcggaccg gaagatcggg
     3541 ctggagccgg accggagcgc gctggagtgg ctcgcggccg agggatacga cagcacctac
     3601 ggcgctcggc cactgaagcg ggtgatccag cggtcgttgc agaacacgct cgccgggttg
     3661 atccttgaag gcgaggtgaa ggaaggcgag accgttcgcg tctcggccgg agccagtgga
     3721 ctggtgatca acgggcagtt ggcggaagcg gcctgacgat ccggggggcg gtttcgtgcc
     3781 gcccccctcc cgctgtcccc ctatcccgct gtccggggtc gggcaaacca ttctccaggc
     3841 cgggcagccg tcaatcccga ccaaacgcct ggtcggaaat gtccgaagcc gccggcaccg
     3901 ccgtatcgga cgacacgaca atccgcgcgt tacccggcag cctgaccaaa tcttccattc
     3961 gccgcgcccg catcccggcc gcgaata
//
LOCUS       DASWQN010000813         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1134018, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000813 DASWQN010000000
VERSION     DASWQN010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134018"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VGC82_13725"
     CDS             complement(<1..759)
                     /locus_tag="VGC82_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEY0424376.1"
                     /translation="MRVPFSGLATMLPRLAGPVCSVALVVLAMLAGLTTPAPAQQLDL
                     SHGGPIDITARDGIEWRQVEQEVIARGDAKAIRGNVTVLADRLTAWYRKKGATQGTQP
                     AGASGPTGASGPAGASGPTGASGLAGDISTEGNEVYRLQADGHVRILTETDHAEGDKA
                     TYDLDQSVLVMTGRDLKLTTPNNVITAKDDLEYWPQKHMAVARGNAVVVTTDAKRIAA
                     DTLVAYTTDNPPPNVAKPEAASAKPSQDPLASSGK"
     gene            complement(759..1445)
                     /gene="lptC"
                     /locus_tag="VGC82_13730"
     CDS             complement(759..1445)
                     /gene="lptC"
                     /locus_tag="VGC82_13730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY0424377.1"
                     /translation="MTGRPLQTGKDEQRSPGRYLLGNASARDRAPPAPGRMAQRRFVI
                     TLTKWVLPLAAMALLALIALWPEIDAATSKARLALKHVSGEIQGGKLIDARYNGVDEK
                     GRPYTVTAATAWQIDAERVGLISPVGDITLQNGTWLSLTAKQGTFIQHQNQLDLVKDV
                     TLYRDDGTTMRTESASIDMKAGAAAGSEPVHVEGPFGVLDAQGFTVMDKGAAIDFPGP
                     SHVILNGAGN"
BASE COUNT          347 a          687 c          686 g          431 t
ORIGIN      
        1 cttgcccgat gaagcgagcg gatcctggga cggctttgct gaagccgctt cgggtttcgc
       61 gacattcggc ggcggattat cggtcgtata ggcgaccaac gtatcggctg ctatgcgctt
      121 ggcgtccgtg gtcaccacca cggcattgcc ccgagcaacc gccatgtgct tttgcggcca
      181 atactccagg tcgtcttttg cggtgatcac attgttcggt gtggtcagct tcaggtcgcg
      241 tccggtcatg accagaacgg attggtccag gtcataggtc gccttgtcac cctcggcgtg
      301 gtccgtttca gtcaggatcc gcacatgccc gtcggcctgc aggcggtaaa cctcgttccc
      361 ttcggtcgag atgtcgccag cgagccctga tgcgccagtt gggcctgatg cgccagccgg
      421 gcctgatgcg ccagttgggc ccgatgcgcc agccggttgc gtgccctggg tggctccctt
      481 cttgcggtac catgccgtca accgatcggc gagaaccgtg acattgccac ggatcgcctt
      541 tgcatcgccg cgcgcgatca cttcctgttc cacctgccgc cactcgatgc cgtcgcgggc
      601 ggtgatgtcg attggcccgc catgcgacag gtcgagttgt tgcgccggcg ccggcgtcgt
      661 cagcccggcc agcatcgcca ggacgaccag tgcaaccgag cacaccggtc cagccaggcg
      721 gggcagcatg gtcgcgaggc cagagaaggg gacgcgcatc aattgccggc cccgttgagg
      781 atcacgtgcg agggtcccgg gaagtcgatc gccgcaccct tgtccatcac agtgaaccct
      841 tgcgcatcca gcacgccgaa cggtccctcg acatgcaccg gttcagagcc cgccgcggcg
      901 ccggctttca tgtcgatgga ggcgctttcg gtccgcatcg tggtcccgtc atcccgataa
      961 agcgtcacat ccttgaccag gtcgagctgg ttctgatgtt ggatgaaggt gccctgcttc
     1021 gcggtcaaag acagccaggt gccgttctgc agcgtgatgt cgccgacagg ggatatcagt
     1081 ccgacccgtt cggcatcgat ttgccacgcc gtcgccgcgg tcacggtgta gggacggccc
     1141 ttttcgtcca ctccattgta ccgcgcgtcg atcagctttc cgccctggat ttcccccgac
     1201 acatgtttca gggccagtcg ggccttgctc gtggcggcat cgatctcggg ccacagcgcg
     1261 atcagggcca acaatgccat tgccgccagc ggcaggaccc acttagtcag cgtaatcaca
     1321 aaccggcgct gcgccatgcg cccgggcgcg ggtggggcgc gatcccgcgc actggcattg
     1381 cccagcaggt agcggccggg agaccgctgc tcgtccttcc cggtctgaag tggacgcccg
     1441 gtcatggcac gcttcgcgtt acgcgggcgc cctgtctctt tggcccgtgt ccctccagct
     1501 tcgtgccgag cgtttggtcg gcgagggcat cgatgttcga tacgtcccag cggcctcgaa
     1561 agagggtcgc gagcgttccg ggttcagtgt gggcgatgaa acaggtcaag atagcggtgt
     1621 cttaaaatgc tttttgcggc ttttctatgg gccggaggag cgagcagggt caaccctgct
     1681 ggattgatac acagttacaa atagataatg ctttcttccg aaacggctgg ccatcgcgtt
     1741 ggatgcgggc tggattcgat gccgcccggt cgtgccgggg acggcggtgt gaccagaaca
     1801 tgttcagggc tgcctgggac agcagagaac gcatcaaacc gcccgatgag catgatggcg
     1861 gctccggcat tccgccggga tcgtcgtctt gtcccatccg ctgacgcggg cgccgctgtc
     1921 catgtccctg ccgcctgtcg cgcaccctgg caccacgcct gcacttcctt atagtgcgag
     1981 acaatcgcag accggggaaa agcgcgccat gacgatcaat ggccgggtcg tgttcagtca
     2041 gatggacgag gtgcgcttcg gcgtgcccgc cgccgaggcc gttgccgaac aagccacgag
     2101 gttcggcgcc gagagggttt tcctgatggt cagcggcacc ctcaatcgga c
//
LOCUS       DASWQN010000814         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_737905, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000814 DASWQN010000000
VERSION     DASWQN010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737905"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..2021
                     /locus_tag="VGC82_13735"
     CDS             345..2021
                     /locus_tag="VGC82_13735"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0424378.1"
                     /translation="MPMMPGKLALLELLKQEGVRIMFGNPGTTELPLMDALAAEQELK
                     YILALQEAPAMGMADGYAQASGQCAVVNLHAAPGLGNAMGMLYDAQKAGSPILVTAGQ
                     HEQRFNFTEPLLWADLPTIARPFVKWSEEVRRLEDLPRAIHRAVKTALAPPTGPVFLS
                     LPGDILTGSADLDLGRPSRVAAGIRGDAGAIGQAAAIIAKASSPVIVAGDAVAQGGAL
                     QELVALAEALGAPVYDEGMASRAVFPSSHPLYRGALVRLPEAIRGMLMQHDLLISVGG
                     DLFTLSLPGDVESMPEGFPVVHLDTDPWELAKNYPEKVSILGEPRVTLPELTEAVLKA
                     RSGMEAEAAAKRLVHVRAEGVASLQKLHAMADAVAERHPIHPLALMQTIGRLLPDDAV
                     VIDETISSGNGLRRFLKSDEAQSFYGLRGGGIGWGLPAAIGVKLALPDRPVVALIGDG
                     SAMYTIQGLWTAARENLRMVFVVINNYSYRILKQRTNAMKGLAAQADTYVAMDLDKPR
                     IDFASVARGLGLTAHKAGSLSDLGDLMAAALAADGPTLIDVEVDRSWKPV"
BASE COUNT          406 a          603 c          726 g          401 t
ORIGIN      
        1 gtccaatccg ttgatagtta caacgaaacg aaatagcaat aattttgtta atcatcttta
       61 tgttattcgg aagcgcctcg ctcaacgagc cgcgagtgct gagctgccat tgcagtggcc
      121 catcggacca gagcgacacg aaataaactg gacaccgata tgatggcatt caaagcggac
      181 agccagaaaa cccgacgaca aagggtcggt gtcccacgat cattgtgctt caaatcagcc
      241 ggcagttctg tatcggcgaa tagcactgat agcctggcgg cttgatatgt tgcaccgagc
      301 cttgtagcgg atcaggacgc cgccgcacgc ttggggaaac caaaatgccg atgatgccgg
      361 ggaaactcgc cttactcgaa ttgctgaagc aggagggtgt gcgaatcatg tttggcaacc
      421 ccgggactac ggagttgccg ctcatggatg cgttggccgc ggagcaggag ctgaaataca
      481 tcctggcgct gcaggaagcg ccggcgatgg gaatggcgga cggttacgcc caggcgtccg
      541 gccagtgtgc cgtggttaat ctgcacgcgg caccgggcct tgggaacgcg atgggcatgt
      601 tgtacgacgc tcagaaggcc gggtcgccga tcctggtgac cgccgggcag cacgaacagc
      661 gcttcaactt caccgaaccg ctgctatggg ccgatctacc gaccatcgcg cgcccattcg
      721 taaaatggtc ggaggaagtg cggcgcctgg aagatctgcc acgggcgatc catcgggcgg
      781 tgaaaaccgc attggcgcct ccgaccgggc cggttttcct gtcgctgccg ggcgatatcc
      841 tgaccggcag cgccgatctg gatctcggca ggcccagccg ggtcgccgcc gggataaggg
      901 gcgatgccgg cgcgatcggg caggcagccg cgatcatcgc gaaggccagc agcccggtga
      961 tcgtcgccgg cgatgcagtc gcgcagggtg gtgcgctgca ggaactggtg gcgctggccg
     1021 aggcattggg cgcgccggtg tatgacgagg gcatggccag ccgagcagtg tttccttcat
     1081 cacacccgct gtatcgtggc gcgctggtgc ggctgccgga ggcgatccgc ggcatgctga
     1141 tgcagcacga cctgctgatt tcggtcggcg gcgacctgtt taccctgtcc ttgcctggcg
     1201 acgtggagtc catgccggaa ggcttcccgg tggtgcatct ggataccgat ccttgggagt
     1261 tggcgaaaaa ctatccggag aaggtttcca tcctgggtga gcccagggtc acgcttccgg
     1321 aactcacgga ggccgtgctg aaggctcgct ccggcatgga ggccgaggcc gcggcgaagc
     1381 gtctggtgca cgtgcgggcc gaaggcgtcg ccagtttgca aaagctgcac gcgatggcgg
     1441 atgcggtcgc ggagcggcat ccgatccatc cgctggccct gatgcagacg atcgggcggt
     1501 tgctgccgga cgacgcggtc gttatcgatg aaaccatctc atccggcaac ggcctgcgtc
     1561 gctttctgaa aagcgacgag gcgcaaagct tctacggcct tcgtggcggc ggcatcggct
     1621 gggggttgcc tgccgcgatc ggagtcaagc tcgcgctgcc ggatcgtccg gtggtggcgc
     1681 tgatcggaga tggatcggcg atgtacacga tccagggcct atggacggcg gcgcgcgaga
     1741 acctgagaat ggtgttcgtt gtcatcaata actattcgta tcgaattctc aagcagcgaa
     1801 cgaacgcgat gaaggggttg gcagctcaag ccgacacgta tgttgcgatg gatctggaca
     1861 aaccgcggat cgattttgcc tcggtggcgc gcgggctggg tctgacggcg cacaaggccg
     1921 ggagtttgtc cgaccttggc gatctgatgg cggcggcatt ggcggcggac ggcccgacct
     1981 tgatcgacgt ggaggtcgat cgttcctgga agccggtctg acgtcgccgg cgccgaatcg
     2041 gtccaacccg tcgagctatc gttggccgca gtcgtcgcgg gacatgccat gctaatcggg
     2101 atcgggagga catcatgaag ttcggttact tcacgc
//
LOCUS       DASWQN010000815         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_737948, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000815 DASWQN010000000
VERSION     DASWQN010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737948"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1055)
                     /locus_tag="VGC82_13740"
     CDS             complement(24..1055)
                     /locus_tag="VGC82_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0424379.1"
                     /translation="MRFGIWALVHGSRGALQDPEEPYDASWERNLALVQEAERLGYDS
                     TLVAQHTINPHHPPNDQLEAWTASAALAAVTSRIEIITAIKPYLYHPVVLAKMALQIE
                     HISRGRFALNLVNAWNMPELEKAGIPFAPHDERYAYGSEWISVVAPLMQGDTVTFRGK
                     YFNVNDYTLRPADPYRARPKIYVGGESEPARALVADHGDVWFINGQPLTDVKILIADV
                     ARRPRQAAPLEFGLSAFVIARKTEAEAQAALLHLAELAKLDAPIRAVQRANTDPYSVM
                     AQTMSKTPRVGSNGGTAAGLVGSYDQVADRIRHFADAGIELFMLQFQPFEAEMRRFAT
                     EIMPRVRRL"
BASE COUNT          283 a          486 c          579 g          329 t
ORIGIN      
        1 ctcagccggt cctgagcgcg gcttcaaagc cggcggacgc ggggcatgat ctccgtcgcg
       61 aagcggcgca tttcagcctc gaacggctgg aactggagca taaacaactc gatgccggcg
      121 tcggcgaagt ggcggatgcg gtcggccact tgatcgtagc tgcccaccag acccgctgcg
      181 gtgccgccgt tgctgccgac gcggggcgtt ttcgacatcg tctgcgccat gaccgaatat
      241 ggatcggtat tggcgcgctg caccgcccgg atcggcgcat ccagtttggc gagttcggcc
      301 aggtgcagca gcgccgcctg tgcctcggcc tcggtttttc gggcaatgac gaaggcggac
      361 aggccgaatt ccagcggtgc cgcctgacgc gggcggcgag ccacgtccgc tataaggatt
      421 ttcacgtctg tcagcggctg gccgttgatg aaccagacgt cgccatgatc cgccaccagc
      481 gctcgggcgg gttccgattc accgccgacg tatattttcg gacgggcgcg atatgggtcg
      541 gcggggcgca gggtatagtc gttcacattg aaatacttgc cccggaaggt gaccgtatcg
      601 ccttgcatca ggggcgccac gacggaaatc cattccgagc cgtaggcgta gcgttcatcg
      661 tgcggagcga aggggattcc ggccttttcc agttccggca tgttccaggc gttgaccaga
      721 ttgagcgcga agcggcctcg gctgatgtgc tcgatctgca gtgccatctt tgccagcacg
      781 accgggtggt agaggtaggg cttgatcgcc gttatgattt cgatgcggct ggtgaccgcc
      841 gccagtgcgg ccgaggcggt ccaagcctcc agctggtcgt tcggcgggtg atgcggattg
      901 atggtgtgct gggcgaccag cgtgctgtcg tagccgaggc gttcggcttc ctggaccaac
      961 gccagatttc gctcccagga ggcgtcgtat ggttcctcgg ggtcctgcaa cgcgccgcga
     1021 ctgccgtgaa cgagtgccca gattccgaag cgcatttgcc aattctccgg tgctgtctgt
     1081 agccttggga cgtgttgctg tcgagaaagc cgtgggataa cggttgggtg caacccagtg
     1141 ccattgtgtc tggccgaggc gttcggacgg agcctgctgg gcgcgtttca tctgggcggc
     1201 ggcgcacgtg gggaccaggc aggcgccgta tcatgatggg tgtggttatg ctgcgatggc
     1261 ctgggtttca gaaacacgcc gaggatttga gcttcggcca gcatggggat gccgtggaat
     1321 tctccgccgt cgatcacgaa ctcattgcgc ggacccgccg cgagaaggcg ggccagcggg
     1381 tcagggccag cggatcagag cgagagtaga ggcggcgcac cggatgcagc atacgcgtcc
     1441 gtcgggccga cgccgatcga tgtgacaacc gatccggacg cgaagccgcc gatcagcttc
     1501 tatgccggcc attatccgga ggcgttcgga tggaccagga tctgaaagat tttgcagcat
     1561 tgatcgatgc cgccgaccga atcgtcctat tcactggcgc ggggatcagc acggagtccg
     1621 gcatcccgga ttttcgcagc cctggcgggg tgtggaccaa gcagacgccg atcgatt
//
LOCUS       DASWQN010000816         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1795066, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000816 DASWQN010000000
VERSION     DASWQN010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795066"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VGC82_13745"
     CDS             <1..571
                     /locus_tag="VGC82_13745"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY0424380.1"
                     /translation="TITCDSAEEAVAGAATLGYPVVLKGVADGVAHKSDLGLVHVGLR
                     DPGMVEDAYAAVGCPTVVVQGMIAGDLEAIAGVTRAEGVGMVLVAGLGGIFAEALHDV
                     ATFPLPVSRSFIEAGVAKSSLGRVLASPRWRHRHAARAFVDLLMNLQATALALGDGLE
                     AIDINPVILGPAGAIAVDALVVPLGVSKP"
     gene            568..1710
                     /locus_tag="VGC82_13750"
     CDS             568..1710
                     /locus_tag="VGC82_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HEY0424381.1"
                     /translation="MSARFTVTYQVRGTAEDIAARAQAIAVEQSVEMPLAAIHDEKIL
                     SEIVGVVEGIDDIGDGSFTVRIGLATETVGHDAGQFLNMIFGNTSLHDDVMLKDVAVP
                     DSLAKVFRGPRHDVAALRSRLGIPARALTGSALKPQGMSADRLGALAAQLASGGLDFV
                     KDDHGLADQDYSRFAGRVRACSAGVGRAARSTGHPTRYIPSVTGDLTVMRSQIAFARS
                     EGLDCVMLAPMICGFPTMQTLRQEFPEMALFAHPSMGGAARIAPELLIGGLFRLLGAD
                     AVIFPSFGGRFGYSSETCRRLADNARRSGDGLKAALPVPAGGMTVERTKEILDFYGPD
                     TLLLIGGNLLMAGDRITAETERFTRAVADHRFANQSVITLDGTSEA"
     gene            complement(1854..>2039)
                     /locus_tag="VGC82_13755"
     CDS             complement(1854..>2039)
                     /locus_tag="VGC82_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424382.1"
                     /translation="RFTEWRAMAEPLVADGSLIYREDVIDGLENAPDALTGILSGRNF
                     GKLLVRVAAERPQASSH"
BASE COUNT          346 a          595 c          706 g          392 t
ORIGIN      
        1 gacgattacg tgcgactcag ccgaggaagc ggtcgcaggg gctgcgacac tgggttatcc
       61 ggtggtgctg aaaggcgtcg ccgacggcgt tgcgcacaag agcgatttgg gtttggtgca
      121 tgtcgggctg cgcgatccag gcatggtgga agatgcctac gcggcggttg gctgtcccac
      181 tgtcgtggtg caagggatga tcgcgggcga tctggaagcc atcgccggcg tgacccgggc
      241 ggaaggcgtg gggatggtgc tggtcgccgg cctgggaggc attttcgcgg aagcattgca
      301 cgacgtcgcc acgttcccac ttccggtcag ccgctcgttt atcgaggctg gagtggcgaa
      361 gtcatccctg gggcgcgtgc ttgccagccc acgatggcgg catcggcacg cggcccgtgc
      421 tttcgtcgat cttctcatga atctgcaagc gactgcgctg gcgctgggcg acgggctgga
      481 agccatcgac atcaatccgg tgatcctggg gccagccggt gcgatcgcgg tcgatgcgct
      541 ggtggtgccc ctcggggtgt caaagccatg agcgcccggt ttaccgtcac ttatcaggtt
      601 cgcggtaccg ccgaggacat cgctgcccgg gcgcaggcca tcgccgtcga gcaaagcgtc
      661 gagatgccac tggccgccat ccacgatgag aagatcctgt cggaaatcgt cggcgtggtg
      721 gagggtatcg acgacatcgg cgatggctcg ttcaccgtgc ggattgggtt ggccaccgag
      781 accgtgggcc acgatgccgg tcagttcctc aacatgatct tcggcaacac gtcgctgcat
      841 gacgacgtga tgctaaaaga tgtcgctgta ccggacagcc tggccaaggt gttccgcggg
      901 ccgcggcacg acgttgccgc cctgcggtct cggcttggaa ttccggccag ggcactgacc
      961 gggtcggcgt tgaagccgca gggcatgtcg gcggatcggc tgggagcgtt ggcggcgcag
     1021 ttggcgagcg gcgggttgga ttttgtcaag gacgaccacg gactggcgga ccaggactat
     1081 tcccgcttcg ctggccgggt acgggcttgc tccgccgggg taggccgagc agcacgttcc
     1141 accggtcacc caacgcgcta tataccgagc gtgacgggtg atctgacggt gatgcgctca
     1201 caaatcgcct ttgctcgaag cgaaggcctc gattgtgtca tgctggcgcc gatgatctgc
     1261 ggctttccca cgatgcagac cttgcggcag gaatttccgg agatggcgct tttcgcccat
     1321 cccagcatgg gcggtgcggc gcgcatcgca ccggagttgc tgatcggcgg cctgttccgc
     1381 ctgctgggag ccgatgcggt tatcttcccc agcttcggcg ggcggttcgg ctattcatcc
     1441 gaaacctgcc gccgcctggc ggacaatgct cgccggtccg gtgatggttt gaaggcggcg
     1501 ttgccggtgc cggccggggg tatgacggtg gagcggacca aggagatcct ggatttctat
     1561 gggccggaca cgttgttgct cattggcggc aacctgctga tggccggcga ccggataacc
     1621 gccgagaccg agcgcttcac ccgtgcggta gcggatcaca ggttcgcaaa ccagagcgtg
     1681 atcacgctgg acggaacgtc cgaagcatga gaatctcccg atcaaataag gaaatagacc
     1741 atttagagag tggcgcgaga ctgaggtccg ccggatatgg cggacttcag atccgggaca
     1801 ccaggcgatt tcgcggtcct gatgacttca gggtggtccg cgatgggttg gtttcaatgc
     1861 gagctggcct gagggcgctc cgccgcgacc ctgacaagca atttgccaaa gttacggccg
     1921 cttaggatgc cggtcagcgc atcgggcgca ttctccagtc cgtcgatcac atcctcccgg
     1981 taaatcaggc tgccatcggc gaccaagggt tccgccatcg cacgccactc ggtgaaccg
//
LOCUS       DASWQN010000817         6681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1002955, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000817 DASWQN010000000
VERSION     DASWQN010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002955"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            802..2220
                     /locus_tag="VGC82_13760"
     CDS             802..2220
                     /locus_tag="VGC82_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424383.1"
                     /translation="MSAAEITTDIPARMDRLPWSRFHLLIVVALGVTWVLDGLEVTIV
                     GSIGPILRDKQTLGLTDQDIGSVAGCYVAGAVVGALLFGWLTDRFGRRLIFNVTLGIY
                     LVGVLASAFAWNFWSFAAFRAATGLGIGGEYAAINSAIDELIPARLRGRIDLIVNGSY
                     WAGAALGAAGSLLLLSGRFVGIDLGWRLGFGIGAVLGSVILFLRRFVPESPRWQITHG
                     REQEAKQNTDDIERRVGKDLPPAKNTLKVHPRKVFGFGLILGAMVGKNSGRSVLALTL
                     MVAQAFLFNAVFFTYGLVLATFYHVDDARTGLYILPLAAGNFLGPLLLGHLFDTVGRK
                     KMIAGTYGLSGLLLIGVAILFGMGVLTAWTQTFCWMVIFFVASAAASSAYLTASEIFP
                     LETRAMAIAVFYALGTLLGGVSAPFLFGYLISTGSQWTVAAGYLGAAALMIAGSICEA
                     FLGIEAAGKSLEDVAQPLSSSA"
     gene            2290..2685
                     /locus_tag="VGC82_13765"
     CDS             2290..2685
                     /locus_tag="VGC82_13765"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY0424384.1"
                     /translation="MTDCLRWSFVGLMLGFSVAGHAADPWAQAEPLTVIMVDNRFEPD
                     HIVFHARQAYELRLENHGKEMHEFTAPAFLTAASIRDKSLLANRGTDVVVQAGKSVRI
                     FLVAPAKGRYDLSCADHDWDGMVGGITVD"
     gene            2826..4763
                     /gene="thrS"
                     /locus_tag="VGC82_13770"
     CDS             2826..4763
                     /gene="thrS"
                     /locus_tag="VGC82_13770"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY0424385.1"
                     /translation="MPAITLPDGSVRQFDASVTGTDVAAAIGPGLARAALAMKLDGKI
                     VDLSSRIDRDSNVVFITRRDPEALEMIRHDAAHVLAEAVQALYPGTQVTIGPAIENGF
                     YYDFARNEPFTPDDFAAIESKMHDIIGRAAKFERRVIDRDEAIAFFQAKGEKYKAQLI
                     QDLPTTETITLYSQGDWVDLCRGPHMRTTADVGSAFKLMKVAGAYWRGDHRNAMLSRI
                     YGTAWRDQKELDAYLHQLEEAERRDHRRIGKDMELFHIQEEAVGSIFWHPKGWKLYRT
                     VEAYLRRRLDAAGYQEVKTPQLLDRSLWEKSGHWEAFRQNMFIAQVENEDKVLALKPM
                     NCPCHIQIFRHGLRSYRELPLRMAEFGSCHRYEPSGALHGIMRVRAFTQDDAHIFCTE
                     AQITPETVRFVELLSTVYRDFGFPDFRVKFADRPEARIGRDEVWDQAEGALRDACAIA
                     GVDYELNPGEGAFYGPKLEFVLRDAIGRDWQCGTLQVDFMLPERLDAEYVGEDGARHI
                     PIMLHRAIFGSFERFIGILIEQYAGRFPLWLAPVQAVVATIVSDADDYARQVAAAFAQ
                     AGLAVNTDLTNQKINAKVREHSLALVPVLAVVGRKEAENRTVALRRLGSQAQEILPLQ
                     EAVARLASEATPPDLITSNVA"
     gene            4874..5395
                     /gene="infC"
                     /locus_tag="VGC82_13775"
     CDS             4874..5395
                     /gene="infC"
                     /locus_tag="VGC82_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY0424386.1"
                     /translation="MAAPPTRDGPRVNEEIRIPQVRLIDQDGEMQGVLTVREAMQRAF
                     AVGLDLVEISPNADPPVCKILDFGKFKYEQQKKKNEAKKKQKVIEIKEIKVRPNIDEN
                     DYQVKMRAMKSFIDEGDKVKVTLRFRGREMAHQDIGVRVLERIRAEMDTTSKVEQMPR
                     MENRQMVMVLSPR"
     gene            5542..6615
                     /locus_tag="VGC82_13780"
     CDS             5542..6615
                     /locus_tag="VGC82_13780"
                     /inference="COORDINATES: protein motif:HMM:NF007504.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HEY0424387.1"
                     /translation="MPTFPPHALKSLAHLVARRMGSEEGEAMQVADHLVRANLAGHDS
                     HGVGMLPTYVRLLREGLLVPNQTADTVLDAGALLVIDARRGYGQRMAADAVRRAIGRA
                     KEVGACVLAFRNSSHIGRIGTYAELAAAEGCAFTAFVNVADHHDVQATWGASEPRLGT
                     NPFCAAVPGTDGPAVMLDMATTTIAAGKARVAYNKGLPVPEGCLIDASGNPTNDPAGF
                     IRDHTGALRAFGTHKGSGLAVMCEIMAAAVAGGQGAFHEAKGGVLNSMLATVIDLSKL
                     GDPQKISADILEVKSHVKSARPAPGFDEVLTPGEPERRYAAKRMEQGIEVDDTTWRDI
                     RAAAETLGITAAEFDQAIGANRG"
BASE COUNT         1238 a         2082 c         2086 g         1275 t
ORIGIN      
        1 cgccaatccg cgggagctcg gtcgtaccaa tcattccagc ggacaagcca ttcggtattt
       61 ttcatctgcc tatcccgtgc gataattctt acgctatcgg aacctaaaat ccgcggccga
      121 atcgcccggc tagcgaactg gccacgccca ccagggcgct gtcgaggttc ccattgatgg
      181 ccctgtaccg ttcatatttt cgtgttcact accggcatgt gcgggctccc gtgagcctgg
      241 gtcggagccc gtagatccgc cctcggggcc catcagggca agctgttcgg caaacgggcg
      301 gtcgccgttc agcacggcaa ccaccttttt caacgcatcg tttcgaccca actcgtaagc
      361 gcggcgcgcc agcgtttgca gcgagacttc gatgccttct agttcggtgt tcggcaaggg
      421 atgtgacctt cttttagcgg tgatacgtaa cgctccgacc ggcagcttag ccagattttc
      481 atcacctgca accgaacata ccgggttacg gagtggcact aattgatgtt tgaaccgtgg
      541 accggttccc gatccggcac cgttaccgcc tgtcagtgga acccaaatcg ctcgcctgcg
      601 ctatgtcgca gcgctcgtag gagcggcgga tgcatgcgcc ggccccatcc acaatcggtt
      661 tggggacgag cctcaatcag ccgcctcgat ttcatcggtt gtgccggcgg cgcggatcac
      721 gagatggccg cgtgaacgca gccatccctc ccgttttcat ggacggccat tcgacatctc
      781 catcgtgcag gacgaagcca gatgagcgca gcagaaatca ccacggatat tccggcacgc
      841 atggatcgcc tgccctggag ccggtttcac ctgctgatcg tcgtggcgct cggggtcacc
      901 tgggtgctgg acggactgga ggtgaccatc gtcggctcca tcgggccgat cctgcgggac
      961 aagcagacgt tgggcctgac ggaccaggac atcggcagcg tcgcgggctg ctacgtggcg
     1021 ggggcggttg tcggtgcgtt gctgttcggc tggctgaccg accggttcgg ccgccggctg
     1081 atctttaatg taacgctggg gatttatctg gtcggcgtgc ttgccagcgc gttcgcctgg
     1141 aatttctggt cgtttgccgc cttccgagcc gcaaccggcc tggggatcgg cggcgagtac
     1201 gcggcgatca actccgcgat cgatgaattg atcccggcgc ggctgcgtgg ccgcatcgac
     1261 ctgattgtca acggcagcta ctgggccggc gccgcgctgg gtgccgccgg ctccctgctg
     1321 ctgctgtcgg gccgctttgt cggcatcgac ctgggctggc gcctgggctt cggcatcggc
     1381 gccgtgctgg gcagcgtcat cctgttcctg cgtcgcttcg tccctgaaag cccgcgctgg
     1441 cagatcaccc acggccgtga gcaggaagcc aagcagaaca ccgacgacat cgaacgaagg
     1501 gtcggcaagg acctgccgcc ggcgaaaaac accctgaagg tccacccccg gaaagtgttc
     1561 gggttcgggc tgatcctagg cgcgatggtc gggaaaaaca gtggccgcag cgttctggcg
     1621 ctgacgctga tggtcgccca ggcttttctg ttcaatgccg tgttctttac ctacggcttg
     1681 gtattggcca ccttctacca cgtcgatgac gcacgcaccg ggctctatat cctgcctctc
     1741 gccgcgggca actttctcgg ccccctgttg ctcggccacc tgttcgacac cgtcggccgg
     1801 aagaaaatga tcgccggcac ctatggcctg tccggtctgc tgctgatcgg cgtggccata
     1861 ctgttcggga tgggcgtgct gacagcctgg acgcagacct tctgttggat ggtgattttc
     1921 ttcgtcgcgt ccgccgccgc cagttccgcc tacctgacgg ccagcgaaat atttccgctg
     1981 gaaacgcggg cgatggcgat tgcggtgttc tacgccctgg gcacgctgct cggcggcgtc
     2041 agcgcgccgt ttctgttcgg ctacctgatc agcaccggca gtcaatggac ggtggcggcc
     2101 ggttacctgg gcgctgccgc cttgatgatc gccggaagta tatgcgaggc tttcctgggc
     2161 atcgaggcag ccggtaaatc gctggaagac gttgcccagc cgctgtccag cagtgcctga
     2221 tcagccggcc aacggacgac ccgaggcttg ccggcgatgg gctatccggc ttctggagtg
     2281 gaacgcgcaa tgaccgattg cttgcgatgg tcgtttgtcg gcctgatgct gggttttagt
     2341 gtcgcgggcc acgctgccga cccttgggcg caagccgaac cgttgaccgt gatcatggtc
     2401 gataaccgct tcgagcccga ccatatcgtg ttccacgccc gccaggctta tgaacttcgg
     2461 cttgagaatc atggcaagga gatgcatgag ttcaccgccc cggccttcct gacggctgcc
     2521 agcatccgtg ataagagcct tctggcaaat cggggaaccg atgtcgtggt gcaggccggg
     2581 aagtcggttc ggatcttcct ggtcgccccc gccaaggggc ggtacgacct tagttgcgcc
     2641 gaccacgatt gggacggaat ggttggtggc atcacggttg attaaggctt tggagcagaa
     2701 gcctggtccc tcgttgccgc gtcccaccct tgaacaggtc acgccaaccg gcgcagcaaa
     2761 gggttgcggc gaggcgcgct gttcgtcata gacaccgcct cttcaactct caggatcagc
     2821 gacccatgcc cgccattacc ctgcccgacg gttccgtgcg ccagttcgac gcctccgtca
     2881 cgggcacgga cgtggccgcc gcgatcggtc ccggcctggc ccgcgccgcc ctggcgatga
     2941 agctcgatgg caagatcgtc gatctgtcct cccggatcga ccgggattcc aacgtcgtct
     3001 tcatcacccg ccgcgacccc gaggcgctgg agatgatccg ccacgacgct gcccatgtgc
     3061 tggccgaggc ggtgcaggcg ctgtatccgg gcacccaggt taccatcggc ccggcgatcg
     3121 aaaacggctt ctactacgat tttgcccgca atgagccttt caccccggac gatttcgccg
     3181 ccatcgaatc gaagatgcac gacatcatcg gccgcgcggc gaagttcgag cggcgcgtga
     3241 tcgaccgcga cgaagccatc gccttcttcc aagccaaggg cgagaaatac aaagcccagc
     3301 tgatccagga tcttccgacg actgaaacca ttaccctgta cagccagggg gactgggtcg
     3361 atctatgccg cggtccgcat atgcgaacca ccgccgatgt cggttcggcg ttcaagctga
     3421 tgaaggtcgc cggtgcatat tggcgcggcg atcaccgcaa cgccatgctg tcgcgtatct
     3481 acggcaccgc ctggcgcgat cagaaggaac tcgacgccta cttgcaccag ctggaggaag
     3541 ccgaacgccg cgaccaccgc cgcatcggca aggacatgga actgttccac atccaggaag
     3601 aagcggtggg ctcgatcttc tggcacccca aaggctggaa actgtatcgc acggtcgagg
     3661 catatctacg ccgccgcctg gatgcggccg gataccagga agtgaaaacc ccacagttgc
     3721 tcgatcgcag cctatgggaa aagtcgggcc attgggaggc gttccggcag aacatgttca
     3781 tcgcccaggt ggagaacgag gataaagtcc tcgcactgaa accgatgaac tgtccctgcc
     3841 acatccagat cttccgccac ggccttcggt cctaccggga gttgccgctg cgaatggcgg
     3901 aattcggctc gtgccatcga tacgaaccgt ccggcgcgct gcatggcatc atgcgggtgc
     3961 gggcgttcac tcaggacgat gctcatatct tctgtaccga ggcgcagatc accccggaaa
     4021 ccgtccgctt cgttgaactt ctttcgaccg tttatcgcga cttcggtttc cccgatttcc
     4081 gcgtcaagtt cgccgaccgt ccagaggcgc ggattggacg ggacgaggtc tgggatcaag
     4141 ccgaaggtgc gttgcgcgac gcctgcgcca tcgctggcgt ggactacgag ttgaaccccg
     4201 gcgagggtgc tttttatggc ccgaagctgg aatttgttct gcgtgacgcc atcggccgcg
     4261 actggcagtg cggcacactg caggttgact tcatgttgcc ggagcggctg gacgcggaat
     4321 atgtcggcga ggacggtgcc cgtcatattc ccattatgct gcaccgagcc attttcggca
     4381 gctttgaacg ctttatcggc atcctgatcg agcagtatgc cggtcgtttc ccgctctggc
     4441 tcgcgccggt gcaggcggtg gtggcaacga tcgtctccga cgccgacgat tacgctcggc
     4501 aggtcgcggc cgcgtttgcc caagccgggc ttgccgtgaa caccgacctg accaaccaga
     4561 agatcaatgc caaggttcgc gagcacagtt tggcgctggt tccggtgctt gccgtcgtgg
     4621 ggcggaagga agcggaaaac cgcacggtcg cgctgcggcg gctgggatct caggcacagg
     4681 aaatccttcc gctgcaggaa gcggttgcca gacttgcatc agaggcgaca cctcccgatc
     4741 ttataacaag caacgtggct tgattttagg cccgttttcc gcccacacta gccagcggcc
     4801 ttcgtcaggc gcatccccgc gccgtcgttg accgtcgaaa cagagacagg agagaaagat
     4861 agccagagga ccaatggccg ccccgccaac ccgcgacggc ccccgcgtca acgaggagat
     4921 tcgaataccg caggttcgcc tgatcgatca ggacggcgag atgcagggag tgctgaccgt
     4981 gcgtgaggcg atgcaacgcg cctttgccgt cggccttgac ctggtggaga tcagcccaaa
     5041 cgccgatccg ccggtctgca agatcctcga tttcggcaag ttcaaatacg aacagcaaaa
     5101 aaagaaaaac gaggccaaga agaagcagaa ggtcatcgag atcaaagaga tcaaggtgcg
     5161 cccgaatatc gacgaaaacg actatcaggt gaaaatgcgc gccatgaagt cgttcatcga
     5221 cgagggcgac aaggtcaagg tgacattgcg cttccgtggc cgcgaaatgg ctcaccagga
     5281 tattggtgtg cgggtgctgg aacgtatccg agcggaaatg gacacgacct caaaggtcga
     5341 gcagatgccg cgcatggaaa accgccagat ggtgatggtg ttgtcgcccc ggtagcctgc
     5401 catggcacgc cccggcggag gcgggaatcc tgcctccgct tcgatgccgc atcgatacct
     5461 ccggttcggg aacgatgtag tgtcggcagg cttcgattca atcaccggaa tgacatttac
     5521 atctgacatc aggcagcctc gatgcccact tttccacccc atgcgttgaa gtcccttgcc
     5581 cacctggtcg cccgccgcat gggcagcgag gagggcgagg cgatgcaggt cgccgatcat
     5641 cttgttcgtg ccaacctcgc cgggcacgac agccatggcg tgggaatgct gccgacctat
     5701 gtccgtttgc tgcgcgaggg gttgctggta cccaatcaga ccgcggacac ggtattggat
     5761 gccggcgcgc tactggtgat cgacgcacgc cgcggctatg gccaacgcat ggccgccgac
     5821 gccgtgcgcc gggcgatcgg gcgcgccaag gaggttgggg cttgcgtcct tgcgttccgc
     5881 aattcctcgc atatcggccg gatcggcaca tacgccgaac tcgccgcggc cgagggatgc
     5941 gcatttacgg cgttcgtcaa tgtcgccgac catcatgacg tgcaggcgac atggggtgct
     6001 tcggagcctc ggctgggcac caatccgttt tgcgccgccg ttcctggaac ggatggtccg
     6061 gcggtgatgc tcgacatggc gacgaccacg atcgctgccg gcaaggcgcg ggtcgcctac
     6121 aacaaggggc tgccggtccc ggagggttgc ctgatcgacg cttccggcaa tcctaccaat
     6181 gacccggcgg gatttatccg cgatcacacc ggcgcgttgc gggccttcgg cacacataag
     6241 ggttcgggac tcgcggtgat gtgcgagatc atggccgcgg cggtcgccgg tggtcagggc
     6301 gcattccacg aggccaaagg cggcgtgctg aattcaatgc ttgccacggt gatcgatcta
     6361 tccaaactgg gtgatccgca gaagatctca gccgatatcc tggaggtaaa gtcgcacgtc
     6421 aaatccgccc gtccggcgcc agggttcgac gaggtcctga caccggggga acccgagagg
     6481 cgctacgccg ccaagcggat ggagcaaggc attgaggtgg acgacacgac ctggcgcgac
     6541 atccgcgcgg ccgccgaaac gctgggtatc accgcggcgg agttcgacca ggcgataggc
     6601 gccaaccgcg gctgatcgcc aacgtccaag ccacccacgg cgccggaacg aagaacccgc
     6661 ccgccgacgg tcttcaaggc a
//
LOCUS       DASWQN010000818         7018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1267541, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000818 DASWQN010000000
VERSION     DASWQN010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267541"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VGC82_13785"
     CDS             <1..1142
                     /locus_tag="VGC82_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424388.1"
                     /translation="LLPLACFVLPLPLAVAVMIPPMTRRRKPAAKWLCWIDLAVSLSV
                     LAATVVIPLDAVASIFAPLLGLDGVLRSCRRRKRGAIPPLERGLALLASLLLVLTVAI
                     PDPFTAWLAMAGAISMSLLSTLRRRWDEAVLAFVGAGIALLGQQLLLAPGPAVFGYFS
                     LFAGFAMIACVVPDLGVVLVVLILRLANLTPWPQAAEALGVGIALIGVLGCATMLTVV
                     QGKPDAPQSPRSRLALLELSQASTAMLSICLGQEDGRFAGLVLLMLLILTRTAARMAD
                     GIVAGVATACLGGVPPLGVFPGLVLVVLALSNHAPWLLLPLAVGFASLLSASLPRHFP
                     GRALDHWPKVAIPSIGWLPLALALFIGYFAPDSVVRWSRILLATH"
     gene            1142..2656
                     /locus_tag="VGC82_13790"
     CDS             1142..2656
                     /locus_tag="VGC82_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HEY0424389.1"
                     /translation="MEAANNVIRTAEAEMCRPWPRHILSHAEWTALAFGSVAEGWVLL
                     SHWAETAQVHALFLNPAAASIVPVSTAVEAGGYPALSAALPEAAWYERMILDLWGHEA
                     AGSADLRHWLDHGNWLLSPPMAPRPEAQPQGEPIMTPAPEQDEAMVLPLGPVWGRLDE
                     PAHVRLSMNGSSVRRAEALLGFSHKGTLTLMRGKSPRAAARYAARLSADATVAHSVAF
                     ALATEVAMGVEAPPRATRLRIVMMEIERIAAHLDNLAEVGRLAGAAMVHARCGLQREI
                     LLRASDAAFGHRLMMDCVVPGGVAADIAEDGPRFILRALGDIASEMPAIRRLHDGPVL
                     SSRLTGVGRTAGRLAAFPDAEGVKGVGGVVGRASGYAFDVRNVFVPGYRELAPRTAAG
                     SDGDAKARQHLRIQEIEESLRLIGLALDMLPAGPVTVALLPVSGEGIACAESIRGDVW
                     HWLRLDHGQIAAVFPRDPGWALWPLAQRVLQNAALDDIDLIRASLALPVSGMDL"
     gene            2659..3150
                     /gene="nuoB"
                     /locus_tag="VGC82_13795"
     CDS             2659..3150
                     /gene="nuoB"
                     /locus_tag="VGC82_13795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEY0424390.1"
                     /translation="MQTPRSEPVGPVDQATTESLMVRLEAAAQIRLGRSLGIRHINTG
                     SCNGCELELRALNNILYDLERFGLRFVESPRHADVLLVTGPLTRNLRTALEQAWDATP
                     EPKWVVAVGDCAVDGGVFKGSYAVLGGTGLSVPVDLTIGGCPPPPARILAGLRTLLEA
                     NAG"
     gene            complement(3409..3651)
                     /locus_tag="VGC82_13800"
     CDS             complement(3409..3651)
                     /locus_tag="VGC82_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424391.1"
                     /translation="MIFAPAVSLLPSLNPPEHTLVITSFKRLIAALAFVLPVAALVTS
                     PVMAASHTKTKPQHASVHKASAHKTHSKKTVAPTAS"
     gene            complement(3733..4590)
                     /gene="hpnD"
                     /locus_tag="VGC82_13805"
     CDS             complement(3733..4590)
                     /gene="hpnD"
                     /locus_tag="VGC82_13805"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEY0424392.1"
                     /translation="MKPTPLGAARADLDAVEAIVRSAGTSFYHGMRLLPPDRRHAMYA
                     IYAFCRIVDDIADEAGTPQEKRLGLSAWRDKIAALYEGRTEGPVTRVLAAAVPRFQLR
                     AEDFLAVIDGMQTDAETVVVAPNLATLDLYCDRVASAVGRLSVRAFGDASPAADQVAH
                     ALGRALQLTNILRDIQEDAARGRIYLPLEYLQDAGVPPDPIAMLTAPGLPTVCSRVAD
                     MAHAYFRKARAAMELCDRTAMKPARLMGATYDAILVALERRGWERLHEQVSLPKWRKL
                     WLASRHGLF"
     gene            complement(4587..5444)
                     /gene="hpnC"
                     /locus_tag="VGC82_13810"
     CDS             complement(4587..5444)
                     /gene="hpnC"
                     /locus_tag="VGC82_13810"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEY0424393.1"
                     /translation="MNQPDKVLVENVETWSGKDRGDENFPVGSPLIARRHRDPIHRFY
                     TFARNADDIADSPILAPEAKLGRLGIMEDVLLGRRSDGSPSATALRASLAATGVTPAH
                     ATDLLIAFRQDATKSRYETIDELYNYCHYSAVPVGRYVLDLHGETHACHSPSDALCTA
                     LQILNHIQDCARDLRELDRCYLPEALLKHFGATVDDLRLPAETPALRRVFLTLLDRID
                     RMNNAASELPESVRDRRLRLETGVIYNLSKRLARRLANDDPISSRVKLGKADVIFSVL
                     SSVFYLARL"
     gene            complement(5441..6556)
                     /locus_tag="VGC82_13815"
     CDS             complement(5441..6556)
                     /locus_tag="VGC82_13815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0424394.1"
                     /translation="MIALAAVVLLIWLYLVFAHGMFWQAGPVLPVSYPLTAPSVAVVV
                     PARDEAWVIEASLRSLLAQDYAGPFRVILTDDNSTDGTGGIARAIADPRLTVITGAPC
                     PAGWSGKLWAVSQGVEEAAAAELVLLTDADIVHEPAHVSALVARIERYNLDLVSEMVK
                     LRCESWAERALVPAFVFFFQLLYPFARVNDGLKATAAAAGGTILVRRRTLQRIGGIES
                     VRGALIDDVALAAAVKKGGRIWLGHSDLARSVRPYPGVGDIWQMIARTAFVQLRYSLP
                     LLIGTTVGMAVTWLLPPALTLFGHGAAFWCGSIAWLMLSACYLPTLRRFGRSPLWAPF
                     LPLVACFYMAATLGSAFNHYRGRGVAWKGRAYQGIAG"
     gene            complement(6553..>7018)
                     /locus_tag="VGC82_13820"
     CDS             complement(6553..>7018)
                     /locus_tag="VGC82_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HEY0424395.1"
                     /translation="IKPTPTGKMILDAAAGRVPAYIDTGLNIVHVDDVAEGHVLALEK
                     GTIGERYILGGENMLLKDILGLVANVVHRRPPSIQLPEAVVWPAAFIMEKLAKVTGIA
                     PLMTRDHLKMARKKMFYSSAKAMSELGYRPRPVRVAVEDAVAWFRTNGMLSG"
BASE COUNT         1231 a         2232 c         2274 g         1281 t
ORIGIN      
        1 cgctgctccc gctggcttgt tttgtcctgc cgctgccatt ggcggtcgcc gtgatgatcc
       61 cgccgatgac gcggcggcgc aaaccggcgg cgaaatggct gtgctggatc gatctggcgg
      121 tatcgctgtc ggtgcttgcg gcgaccgtgg tcatcccact ggacgcggtc gcatcgatat
      181 ttgccccgct gcttggcctt gacggcgtgc tgcgatcctg ccgccggcgc aagcgcggcg
      241 ccatcccgcc gctggagcgc ggtctggcac ttctggcgag cctgttgctg gtgttgaccg
      301 tggcgatacc ggatccgttc accgcatggc tcgcgatggc gggcgctatt tcgatgagtt
      361 tgctttcgac cctgaggcgg cgctgggacg aagcggttct ggctttcgtc ggtgcgggca
      421 tcgccttgct gggtcaacaa ctattgttgg caccgggtcc tgccgtgttc gggtatttca
      481 gccttttcgc ggggttcgcg atgattgcct gcgtcgttcc ggatcttggc gtggtgctgg
      541 tcgtcctgat cctccgtctc gcgaacctga cgccgtggcc acaggcagcc gaggcccttg
      601 gggttggcat cgcactgatc ggcgtgctgg gctgcgccac gatgctgacg gtggtccagg
      661 gaaagcctga tgctccgcag tcccctcggt cgcggctggc attgctcgaa ctgagtcaag
      721 ccagcactgc catgttgtcg atatgccttg gacaggagga tggacgcttc gccggtctgg
      781 tcctgcttat gttgctgatc ctgacccgaa ccgccgccag gatggccgat gggatagtcg
      841 ccggcgtggc gaccgcttgc ctggggggcg ttcctccact cggtgtgttc cctggtctcg
      901 tgctcgtcgt cctggccctg tcgaaccatg ccccgtggct gttgcttccg ctggcggtcg
      961 gcttcgcgtc cctcctgtcg gccagcctgc ctcgccattt tcccggccgc gcgttggacc
     1021 attggcccaa ggtcgcgatc ccctcgatcg gctggctacc cttggccctt gcgcttttca
     1081 tcggttactt cgcgccggac agcgtggtgc gctggtcccg cattctcctg gcaacccact
     1141 gatggaggcg gcgaacaacg tcatccgaac cgccgaggcg gaaatgtgcc ggccgtggcc
     1201 gcgccatatc ctgtcccatg ccgaatggac cgccctcgca ttcggatcgg tcgcggaagg
     1261 ctgggtcctg ctttcgcact gggcagagac ggctcaggtg catgcgctgt tcctgaaccc
     1321 ggcagcggcg tccatcgtgc cggtttcgac tgccgttgag gccggcggct acccggcttt
     1381 gtccgctgcc ctgcccgaag ctgcctggta cgaacgaatg atcctcgatc tttgggggca
     1441 tgaagccgcc ggctccgccg accttcgcca ctggttggat catggcaact ggctgctgtc
     1501 gccgccgatg gcgccacggc cggaagcgca gccgcaaggc gaaccgataa tgacaccggc
     1561 gccggagcaa gacgaagcga tggtgctccc gctgggaccg gtctggggcc ggttggatga
     1621 acccgcgcat gttcgcctga gcatgaacgg ttcttccgtc cggcgcgccg aagcgttgct
     1681 tgggttctcc cacaagggga cgctgacgct gatgcgcggc aaatcgcccc gcgccgccgc
     1741 ccgctatgcc gcgcggcttt ccgccgatgc aacggtggcg cactcggtcg cctttgccct
     1801 tgccaccgaa gtcgccatgg gtgtcgaggc gccgccccgg gcaacaaggc tgcgcattgt
     1861 catgatggag atcgagcgga tcgccgccca cctggacaac ctggcggagg tcggccggct
     1921 ggcgggggcc gcgatggtgc acgcaagatg cggcttgcag cgcgaaatcc tgctgcgggc
     1981 atcggatgcc gcgttcggtc accggctgat gatggattgc gtcgtgccag gcggcgtcgc
     2041 cgccgacatc gccgaggacg gaccccggtt catcctgcgc gcactcggcg acattgcgtc
     2101 cgaaatgccc gcgatccgcc ggctccatga cggcccggtc ttgtcgtccc ggctgaccgg
     2161 cgttggccga acggccggac gtctggccgc cttcccagac gcggaaggcg tgaaaggcgt
     2221 ggggggcgtt gtggggcgtg catcggggta tgcgttcgac gtgagaaacg tgtttgtccc
     2281 gggctaccgc gaactggccc cgcggaccgc agccgggtcg gacggggacg ccaaggcgag
     2341 gcagcatctg cgtattcagg agattgagga aagcctccga ctgattggcc tcgcgttgga
     2401 catgctgccg gcgggaccgg tgaccgttgc gctgctgcca gtcagcggcg agggcatcgc
     2461 gtgcgccgag tcgatccggg gcgatgtatg gcactggctg cgcctcgatc atggccagat
     2521 cgccgccgtg ttcccgcgtg atcccggatg ggcgctttgg ccgctggccc aaagggtgct
     2581 gcaaaatgcc gcgctggacg acatcgacct gattcgcgcc tcgcttgcgc tgccggtatc
     2641 cggaatggat ttatgatcat gcaaacacct cgatccgaac cggtcggacc ggtcgatcaa
     2701 gcaaccaccg aatcgctgat ggtgcggctt gaagcggcgg cacaaatccg cctgggacgc
     2761 agcctgggga tccgccacat caacaccgga agctgcaatg gctgcgaact ggaactacga
     2821 gcgctgaata acattctcta cgacctggag cgctttggtc ttcgtttcgt cgagagcccg
     2881 cgtcacgccg atgtattgct ggtgaccgga ccactgacgc ggaacctgag aaccgcactg
     2941 gaacaagcat gggacgcgac cccggagccg aaatgggtgg tagccgtcgg cgattgcgcg
     3001 gtggatgggg gtgttttcaa gggcagttat gcagtcctgg gcgggaccgg cctgtcggtg
     3061 ccggtggacc tgacgatcgg cggctgcccg ccaccgccgg ccaggatctt ggccggcctg
     3121 aggacgttat tggaagcgaa tgccggatag cagcaagcct tccccgcgga cgggaaaggc
     3181 tcgttgacgt ccatcagagc atctacgcac gcgtgggagt atgatcacgc tgggcggaac
     3241 gtccgaagcg tgtgaatcat acggtaaaac aaagaggcag agcattttcg gcctgtacaa
     3301 tcggcctgaa aatgctctgg agtatgatca cgctgggcgg aacgtccgaa gcgcatgaat
     3361 catacataaa aaaccatctg cggcctgcac ggtcaggctg aagatgttct agctggcggt
     3421 cggggcgacg gtcttcttgc tgtgcgtctt gtgcgcgctg gctttgtgga cagacgcgtg
     3481 ttgcggcttc gtcttggtgt ggcttgcggc catgaccggt gacgttacca gggcagcgac
     3541 cggcagaacg aatgccagcg cggcgatcaa gcgcttgaac gaagtaatca cgagcgtgtg
     3601 ctccggtggg ttgagggagg gcaaaaggct gacagccggg gcgaagatca tgccctagtc
     3661 gactgacaac actatgcatc gcgcatcggt acgaatgcaa gttatttagc tcgagagaat
     3721 gtgacagtgt ctttaaaaca acccatggcg agaggccagc caaagtttgc gccattttgg
     3781 caaactgacc tgctcatgga gccgttccca gccccggcgt tccagtgcaa cgaggatcgc
     3841 gtcgtatgtc gcacccatca atcgggccgg cttcatcgcg gtgcggtcgc acaactccat
     3901 cgcggccctg gctttgcgga aataggcgtg cgccatatcg gctacgcggc tgcagacagt
     3961 cggcagcccg ggcgcggtca gcatcgcgat gggatcgggc ggcacgccgg catcctgcag
     4021 gtattcaagc ggcaggtaga tccgccccct tgccgcatcc tcttgaatat cccgaagaat
     4081 attagtcaac tgcaaggctc ggccgagcgc atgggcaacc tgatctgcgg ccggagatgc
     4141 atcgccaaac gcccgaacgg acaaacgtcc gacagccgag gccacgcggt cgcagtaaag
     4201 atcgagcgtc gccagattcg gcgccacgac caccgtttcc gcatcggtct gcattccatc
     4261 gatcacggcg aggaaatcct ccgcccgcaa ctgaaaccgt ggtaccgcgg cggcaagcac
     4321 gcgggtcacc ggcccttccg ttcggccctc atacaaggcg gcgattttgt cccgccaggc
     4381 agacaggcca agacgtttct cttgcggcgt tcccgcctca tcggcgatgt catcgacgat
     4441 ccggcagaaa gcatagatcg catacatcgc gtgccggcga tccggtggaa gcaggcgcat
     4501 tccgtggtag aacgacgtgc ccgcggaacg cacgatcgcc tccaccgcgt ccagatccgc
     4561 cctggcggcg cctaacggcg tgggcttcat agtcttgcca agtagaacac cgacgacagg
     4621 acactgaaga tcacgtccgc cttacccaac ttcacccggc ttgatatcgg gtcgtcgttg
     4681 gcaagccggc gtgccagtcg tttcgacaaa ttgtagatga ccccggtttc cagacgcagg
     4741 cggcggtcgc ggacgctctc cggcagctcc gacgccgcgt tgttcatcct gtcgatgcgg
     4801 tccaacaacg tgaggaacac gcggcgaagc gcgggcgttt cagccggcag ccgcaaatcg
     4861 tccaccgttg cgccgaaatg cttcagcagc gcctcgggca agtagcaacg atccagttcg
     4921 cgaagatccc tggcgcaatc ctgaatatgg ttcaggatct gcagtgcggt gcagagggca
     4981 tcggaaggcg aatggcacgc gtgggtttcg ccgtgcaggt ccagcacata gcgtccgacc
     5041 ggaaccgccg aatagtgaca gtagttataa agttcgtcga tcgtctcgta ccgtgacttg
     5101 gtggcgtcct ggcggaaggc gatcagcagg tccgtcgcat gcgccggcgt cacgccggtt
     5161 gccgcgaggc tggcccgcag ggccgttgca ctcggcgatc cgtccgaacg gcgcccgagc
     5221 agaacgtctt ccatgatccc gaggcggccg agcttcgcct ccggggcaag gatcggtgaa
     5281 tcggcgatgt cgtcggcgtt gcgggcgaag gtatagaacc ggtgaatggg atcgcgatgc
     5341 ctgcgcgcga tcaagggcga gccgacggga aaattctcgt ctccgcggtc ctttcccgac
     5401 caggtctcca cattctcgac gaggaccttg tccggctggt tcatccggcg attccctggt
     5461 aggcgcggcc cttccacgcg acgccgcgcc cacgataatg gttgaacgcc gatccgagcg
     5521 tcgccgccat gtagaaacag gccaccagcg gcaggaaggg cgcccaaagc ggcgaacggc
     5581 cgaaccggcg caatgtcggc agataacagg ccgacaacat cagccacgcg atcgagccgc
     5641 accagaaggc agcgccatgt ccgaacagcg tcagcgccgg cggcaacagc cacgtcacgg
     5701 ccatgccgac tgtcgttccg atcagcaacg gcaaggagta gcgaagctga acgaaggcgg
     5761 tgcgcgcgat catctgccag atgtcgccga cgcccgggta tggccgcacc gacctcgcca
     5821 ggtcggaatg gcccagccag atccggccac cctttttaac cgccgccgcc aaagcgacat
     5881 cgtcgatcaa tgcccccctt acgctctcga tgccaccgat ccgctgcagg gttcggcgcc
     5941 tgaccaggat ggttccgcca gccgcggcgg ccgtggcctt caggccgtcg ttcacccggg
     6001 cgaaggggta aagcaactga aagaagaaaa cgaaggctgg caccagcgcc cgttccgccc
     6061 agctttcgca cctcaatttc accatttcgg agaccagatc aaggttgtat cgctcgattc
     6121 tggcaacgag ggcggacaca tgagccggtt cgtgcacgat atccgcatcg gtcaacagga
     6181 ccagttcggc ggcggcggct tcttccacgc cttgcgaaac cgcccacagc ttgccgctcc
     6241 atcctgccgg gcagggtgcg ccggttatca cggtcagccg gggatcggcg atcgcgcggg
     6301 cgatcccacc agtaccgtca gtgctgttgt cgtcggtcag gatgacccgg aacggcccgg
     6361 catagtcctg tgcgagcagt gaccgcaggg atgcctcgat gacccaagcc tcgtcgcggg
     6421 ccggcacgac gaccgcgacc gatggcgccg tcagcggata actgaccggc agcacgggcc
     6481 ccgcctgcca gaacatgcca tgggcaaaca ccagatagag ccagatcagc agcaccacgg
     6541 ccgccagcgc gatcatccgg acaacatgcc attggtccgg aaccaggcaa cggcgtcctc
     6601 caccgccacg cggaccgggc ggggccggta ccctagttcc gacatcgcct tggccgacga
     6661 gtagaacatc ttcttgcgag ccatcttcag gtggtcgcgc gtcatcaacg gggcgatgcc
     6721 ggtaaccttg gccagctttt ccatgatgaa ggccgccggc cagacgaccg cttccggcaa
     6781 ctggatggag ggcggccggc gatgcacgac gttcgccacc agcccgagga tgtccttcag
     6841 cagcatgttc tcgccgccca ggatgtagcg ttcgcctatc gtgcccttct ccaaagccag
     6901 gacatggcct tcagcgacat cgtccacatg aacgatgttc aggccggtat cgatgtaggc
     6961 cggcacccgc ccggcagccg cgtccaggat catcttgccc gtgggggtcg gcttgatg
//
LOCUS       DASWQN010000819         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1003079, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000819 DASWQN010000000
VERSION     DASWQN010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003079"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..366)
                     /locus_tag="VGC82_13825"
     CDS             complement(211..366)
                     /locus_tag="VGC82_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424396.1"
                     /translation="MTTRRTLVLSDPPVPPGDPPVLPDPDEPVPVEEPPSPIPIPPDG
                     PPEPLRA"
     gene            complement(530..>2014)
                     /gene="glgX"
                     /locus_tag="VGC82_13830"
     CDS             complement(530..>2014)
                     /gene="glgX"
                     /locus_tag="VGC82_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY0424397.1"
                     /translation="EMVARLHDAGLEVILDVVYNHTAEGNENGPTLSFRGIDNLSYYR
                     LMPEQRYYINDTGTGNTLNMSHARVVQMVTDSLRYWATEMHIDGFRFDLGTILAREAD
                     GFNEAHGFLQSCLQDPILSATKLIAEPWDIGPGGYQVGGFPPGWAEWNDKFRDTTRAY
                     WKGDPGMQAEMATRMAASADSFARRGRKAWASVNFITAHDGFTLNDLVSYNEKHNEAN
                     GEEGRDGHSNNHSWNFGVEGPTDDPGIIALRERQKRNLLATLLLAQGTPMLLAGDEFG
                     RTQNGNNNAYCQDNELSWVNWNFGEREVELAEFARKVIVLRQSFPILRRSRFLTGEYN
                     ADLDVRDVRWMSPSGQDIEEAHWGDANARCFGMLLDGRAQASGIKRPAMDATALLVLN
                     AHHDVVNFAMPDVVGGSVWRCLLDTNNLDDVNQPFQTGETYEITGRSLVLFVLEPESR
                     RSVALRRAIEGFRQVAERPIPVATPEPGPETEMALSPGTGTTRP"
BASE COUNT          373 a          596 c          634 g          411 t
ORIGIN      
        1 gttgcagcaa agcagttgtt tacgcgacgc ccgaggccgc aggcttttat cagaccgccg
       61 gttatgatga ggaagactgg gacgagatct gcatgggcgg catcgtgcaa atgctcaaga
      121 agctggactg acgaagcgag ggcaggcgtg ccgatgccgc gctgtcagcg cgtttacagg
      181 tttaccgaca acgcagcatg gcgaggctga ttatgcccgc aatggctccg gcgggccatc
      241 gggcggaatc ggtatcggcg acggcggttc ctcgaccggc accggttcgt ccgggtccgg
      301 caagaccgga ggatcgccag ggggaaccgg cggatccgac agaaccaggg ttcggcgcgt
      361 cgtcaatggt ggttccattc cggttgtttc tgacaccata tgtcctcacg taggaaagcc
      421 gcgtcggcgt tcgagggttc gccggggatc gatgcaccag accaggaaag ccagcggttt
      481 gacggtcgtc gcgtctgcag cataacccgc ggccttttag ctccgtcatt cacggacgtg
      541 tcgttccggt tcccggcgac agggccattt ccgtttcggg accgggctca ggtgtcgcca
      601 ccggaatcgg acgttcggcg acttggcgga aaccctcgat cgcgcgccgt agtgcgacgg
      661 agcgtctgct ttccggctcc agcacgaaca gcaccagcga gcgtccggtt atttcatagg
      721 tttcacccgt ttgaaacggt tgattcacgt catcgaggtt gttggtgtcc agcagacagc
      781 gccaaaccga gccgccgacg acgtccggca tggcgaagtt caccacgtca tggtgggcgt
      841 tcaggaccag aagcgccgtg gcatccatgg ctggccgctt gatgccgctg gcttgcgctc
      901 gcccgtccag cagcatcccg aaacaccggg cattggcatc gccccagtgg gcctcctcga
      961 tatcctgacc cgacggggac atccagcgga catcgcgaac atccagatcg gcgttatatt
     1021 ctccggtcag aaagcgagat cgccgaagga tcggaaagga ctgccgcaac acgatgacct
     1081 tgcgagcgaa ttcagcgagt tcgacctccc tctcaccgaa attccagttt acccagctaa
     1141 gctcgttgtc ctggcaatac gcgttgttgt tgccgttctg cgtacgaccg aactcgtcgc
     1201 cggccagcag catcggcgtg ccttgagcca gcaaaagcgt agccagcaaa ttgcgtttct
     1261 gccgttcgcg caaggcgatg atgccggggt cgtcggtcgg gccctctacg ccaaagttcc
     1321 aggaatgatt attggaatga ccgtcgcggc cctcctcgcc attcgcttcg ttatgtttct
     1381 cattgtagga aaccaggtcg ttcagggtaa agccgtcatg ggcggtgata aagttcacgc
     1441 tggcccacgc cttgcggcca cgtcgggcga aagaatcggc ggatgcggcc atgcgagtcg
     1501 ccatctcggc ctgcatcccg ggatcgcctt tccaataggc tcgggtggta tcgcggaatt
     1561 tgtcattcca ttcggcccag ccgggcggga aacctccgac ctgatagccg ccgggtccga
     1621 tgtcccatgg ttcggcgatt agttttgtcg ccgacaggat gggatcctgc agacagcttt
     1681 gcagaaaacc gtgcgcttcg ttgaacccgt cggcttcgcg cgccaggatc gtgccgagat
     1741 cgaagcggaa tccgtcgatg tgcatctcgg tcgcccagta gcgcaggctg tcggtgacca
     1801 tctgcacgac gcgggcgtgg ctcatgttga gcgtgttccc ggtgccggtg tcgttgatgt
     1861 agtagcgctg ttcaggcatc agccgatagt acgacagatt gtcgatgccg cggaaagaca
     1921 gggtcgggcc attctcgttg ccttccgccg tgtggttgta aacgacgtcc aggatgactt
     1981 ccaggccggc gtcgtgcagg cgggcgacca tctc
//
LOCUS       DASWQN010000820         7528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2191342, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000820 DASWQN010000000
VERSION     DASWQN010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2191342"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..913
                     /locus_tag="VGC82_13835"
     CDS             125..913
                     /locus_tag="VGC82_13835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0424398.1"
                     /translation="MTQGSTVTQGFAIYPSLRDRVVLVTGGASGIGAEEVTQFARQGA
                     KVAFLDVSDDAARQLGDTLGGESLPRPHYVHCDLKDIPAVQAAIAEIAGRLGPITVLV
                     NNAANDQRHKFEDVTVEFWDERMATNLRHQFFTIQAVAPMMRAAGGGSIINFGSISWH
                     TSQGGMPAYTTAKAGIEGLTKGMARDLGPHGIRVNCVIPGWIMTQRQIDLWLTPEAEA
                     NLLQAQCLKAKLVPADVARMVLWLASDDSRMCSSQLWIVDGGRL"
     gene            complement(947..2455)
                     /locus_tag="VGC82_13840"
     CDS             complement(947..2455)
                     /locus_tag="VGC82_13840"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEY0424399.1"
                     /translation="MTNPTAYERLASRFKLISTIGECASMLGWDAAAVMPSGGGAARG
                     DQLAVLAVLRHGHLIAPEVEADLAAAEGTNAWEAANIRLMRHEYTRAKALPPDLVEAQ
                     AKANSDCEKVWRVARGDSDFKLVQPYLAEVLRLTREAATVLSTALGLSPYDALMDGYQ
                     QGVGADDVAPIFADYEEFLSSALPFAEELQARQPAPIAPAGPFPIAAQQALCRDLAQR
                     LGLDFEHARLDQSAHPFSGGTPTDVRITTRYQEEDFASAILAVVHETGHALYERGLPS
                     AYARLPVGEAAGMAVHESQSLIVEMQAFRSDSFLGWLGGYLHDTFGGAPEPYSLDNLG
                     RLWRKVQRSHIRVDADELTYPAHVIQRFRLERAMIAGDLGVADLPVAWNDALKSLLGI
                     TPANDAQGCLQDIHWYDGAFGYFPSYTLGAMSAAQLMRAAGEQVAGLDAALGRGDLSP
                     LLAWLRVNVHSVGSRLDMNEILERATGRRLDPLAFQDHLKRRYLEPSGARAV"
     gene            complement(2452..3144)
                     /locus_tag="VGC82_13845"
     CDS             complement(2452..3144)
                     /locus_tag="VGC82_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEY0424400.1"
                     /translation="MTAGYGRFVVPGLSTLVMMVVLIGLGTWQVQRLHWKQTILEQIA
                     KAEAAPAVPLMDAPAPYTKVSVTGRFQFDRAAQFGVEVRDTRAGPTIGFDQIVPLDRD
                     GAPPLLVDRGWVPLKRQTPLDDPAGAVTIVGYVRPGEKPSWFSASDDVPARQFFTLDP
                     RAIGNAVGVRDPEPFTLVALGQATAEVYPAPTQHLPRPLNNHLSYVITWYGLAGALAV
                     ISGAWMRKALRS"
     gene            complement(3350..4198)
                     /locus_tag="VGC82_13850"
     CDS             complement(3350..4198)
                     /locus_tag="VGC82_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY0424401.1"
                     /translation="MSSDAHSAPSLATSGIKHPYHLPDPSPWPIIGSLGGFLTVFGIV
                     LAAHYGNYVVLVLGLLTVLTTMFFWWRQVIRESRTPGAHSVIVRLGLRYGMTLFIASE
                     VTFFVGFFWSYFNFLLFPETQGDGQTVWPPTNVHTFDPFHLPFLNTMILLLSGTTVTW
                     AHHSLLEGDRKGLVRGLGITILLGLAFTTCQAIEYSNAPFKFNGGGVYSSVFFLATGF
                     HGFHVIVGTCFLIVCWFRARGGQFTPERHFGFEAAAWYWHFVDVVWLFLFICIYWYGA
                     GQIAAE"
     gene            complement(4293..4913)
                     /locus_tag="VGC82_13855"
     CDS             complement(4293..4913)
                     /locus_tag="VGC82_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY0424402.1"
                     /translation="MTASQRNALLGGTVALAVVGMVGMSFAAIPLYRLFCAATGYAGT
                     PGIGPAPAPGSSGGTIRVRFNADTNPGLPWIFAPDQLEVSVNLGDEQVAFYHARNQSS
                     QPVTGMALYNVTPEKVGKYFHKTACFCFNKQMLMADQSMEFPVSFWVDPAIRADPNTA
                     DVKTITLSYTFFRSLGDAAASGALAKAGPHVGGTSAAEPEQESRTN"
     gene            complement(5086..6063)
                     /locus_tag="VGC82_13860"
     CDS             complement(5086..6063)
                     /locus_tag="VGC82_13860"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY0424403.1"
                     /translation="MIDATPSMIHANEGDSPPLAAALGPTRDLCLESDYRDWIALLKP
                     RVLTLVVFSGAIGLAVAPGHLHPVLAFTAILCIAIAAGACGAINMWYDRDIDAIMRRT
                     RNRPIPAGRIQPGPALGFGVTLAVGSVLMMGLALNIPAALILALSICFYVFVYTVWLK
                     RRTPQNIVIGGAAGAFPPVIGWAAVTGSVDVIPLVLFAIVFVWTPPHFWSLALWANDD
                     YRRAGIPMLPVVAGAKETRRQILLYTLVLVPLTLVPFFMGFSGLPYGLTAGLLGVVFL
                     QRVYQVLTDRQDAAGNSLTGDVPAKAAFKYSVLYLFALLGALAIDRLAG"
     gene            complement(6067..>7528)
                     /gene="ctaD"
                     /locus_tag="VGC82_13865"
     CDS             complement(6067..>7528)
                     /gene="ctaD"
                     /locus_tag="VGC82_13865"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY0424404.1"
                     /translation="LVGGVLSQIMRAQLQFPHNTIVPDGQEWNTIITSHGLLMIFFSV
                     MPALIGGFGNWFIPLMIGAPDMAFPRLNNISFWLLPPAFVLICIGLVMNESGSGWTLY
                     PPLSNATYENGMGMDCTLFALHLAGVSSLLGAMNFIVTIFNMRAPGMTLHKMPLFIWS
                     ELVTAFLLVLAVPVLAGAITMLICDRNFGTAFFDPTGGGDPVLFQHLFWFFGHPEVYI
                     MILPGFGIISHVVSTFSKKPIFGYLGMVYAMIAIGVVGFVVWAHHMFVSGIDVDTRAY
                     FMAATLVIAVPTGIKIFSWIATMWGGSIELKTPMLWAIGFIFVFTIGGVTGVVLANAG
                     IDQVVHNTYYVIAHFHYVLSLGAVFSIFAGFYYWIGKMSGHQYPEFWGKVHFWLTFIG
                     VNLTFFPQHFLGLSGMPRRYPDYPEAFAGWNFVSSIGAYISGIAFLVFLYVCFRTFTS
                     KEHVADNYWGQGATTLEWTQTSPPAFHTYLEMPRIP"
BASE COUNT         1680 a         2308 c         2306 g         1234 t
ORIGIN      
        1 tggatagcca gttcgcgact ctcgaagagc cggcacaaga cgagcatcca atcatcgtgg
       61 acatcggcgg atcggccgag gcggccattg agaaagcaat cagtcaaatg aaggaacgtt
      121 taccgtgact cagggatcta ccgtgactca ggggttcgcc atctacccaa gcctgcgcga
      181 tcgggtcgtc ctggtaaccg ggggcgccag cggcatcggc gccgaggagg tcacccagtt
      241 cgctcgtcaa ggcgcgaagg tcgcgtttct ggatgtatcg gatgacgcgg cacgccagct
      301 tggcgacaca ctgggtgggg aaagcctgcc acggccgcat tacgtgcatt gcgacctgaa
      361 agatattccc gccgtccagg ccgcgatcgc ggaaatcgcc ggacggctcg gcccaattac
      421 cgttctggtc aacaacgccg ccaacgacca gcgccataag ttcgaggatg tgacggtcga
      481 attttgggat gagcggatgg cgaccaattt gcgccatcag ttctttacga ttcaggctgt
      541 cgcaccgatg atgcgtgctg ccggtggggg atcgatcatc aatttcggct cgatttcctg
      601 gcataccagc caaggcggaa tgccggccta caccaccgcg aaagccggca tcgagggcct
      661 gaccaaaggc atggcgcgcg atcttggccc gcacggcatc cgggtcaact gcgtgattcc
      721 aggttggatc atgactcagc gacaaatcga cctgtggttg acgccggaag ccgaagccaa
      781 tttgctgcag gcccagtgtt taaaagccaa actggtgccc gccgatgtcg ctcgcatggt
      841 gctctggctg gcttccgacg acagccgcat gtgcagcagc cagttgtgga tcgtggatgg
      901 cggccgcctg taaacccatg ctcaaactta cggccaacgg ggtcggttag acagcccgag
      961 cgccggatgg ttcaagatag cgccgcttga gatgatcctg aaatgcgagc ggatccaatc
     1021 ttcggccggt tgcccgctcc aggatttcgt tcatatccag ccggcttccc acgctgtgca
     1081 cattgacgcg tagccatgcc aaaaggggcg acaggtcgcc acggcccaaa gccgcgtcga
     1141 gccccgccac ttgctcgccc gcggcccgca tcaactgcgc cgccgacatc gcgcccagcg
     1201 tgtagctcgg gaaatacccg aacgcgccat cataccagtg gatgtcctgc aagcacccct
     1261 gcgcatcatt tgccggcgtg atgccgagca gcgacttcag ggcgtcgttc caggccacgg
     1321 gaaggtcggc aacgccgaga tcgccggcga tcatcgctcg ctcgaggcgg aagcgctgga
     1381 tgacatgggc gggataggtc agttcgtctg catcgacgcg gatatggctg cgctgaactt
     1441 tacgccatag ccgtcccaga ttgtcgagac tgtaaggctc aggggcgccc ccgaacgtgt
     1501 cgtgaaggta gccgccaagc cagcccagaa aagagtcgga acgaaaggcc tgcatctcga
     1561 cgatcagcga ctggctttcg tggaccgcca tgcccgcggc ttcgcccacc ggcaaacggg
     1621 catacgccga tggcaggccg cgctcataaa gcgcatgacc ggtttcatgc acgacggcga
     1681 gaatggcgga ggcgaagtcc tcctcctgat agcgggttgt gatgcgcaca tccgtaggcg
     1741 ttccgccgga gaacggatgg gccgactggt ccagccgcgc atgctcgaag tccaggccca
     1801 ggcgttgggc aagatcgcgg cagagcgcct gctgagcggc aatcggaaag ggtccggccg
     1861 gcgcgatagg cgcgggttgc cgcgcctgga gctcctccgc aaacggcaat gccgaggaaa
     1921 gaaactcctc ataatccgcg aagatcggag cgacatcatc ggcgccaaca ccttgctggt
     1981 agccgtccat cagcgcgtcg taggggctca ggccaagggc ggttgacagg acggtggcag
     2041 cctcacgggt cagacgcagt acttccgcta ggtagggctg gaccagcttg aaatcgctgt
     2101 cgccgcgcgc gacccgccac accttttcac agtccgagtt cgccttggcc tgggcctcca
     2161 ccagatccgg cggcagcgct ttcgccctgg tatattcgtg ccgcatcagg cggatattcg
     2221 ccgcttccca cgcattggtc ccctcggcgg cagcaagatc cgcctcgacc tccggcgcaa
     2281 taagatgccc atgccggaga actgcgagca cggcgagttg gtcgcctcgg gctgctccgc
     2341 cgccggacgg catgaccgcg gccgcgtccc accccagcat cgatgcacac tcgccgatgg
     2401 tgctgatgag tttgaagcgg ctcgcaagcc gttcgtaggc ggtcgggttg gtcatgatcg
     2461 caacgccttt ctcatccatg caccagatat gacggccagc gctcccgcca ggccgtacca
     2521 ggtgatcaca tagcttagat gattgttcag cggccgtggc aaatgctgcg tcggggcagg
     2581 gtacacttcg gcggtcgctt gacctagcgc caccagggtg aacggttcgg ggtcccggac
     2641 gccaaccgcg ttgccgatcg cccgcggatc tagcgtaaag aattgcctcg ccggcacgtc
     2701 gtcggatgcg ctgaaccagc tgggcttttc tccgggtcgg acataaccca cgatggtaac
     2761 ggcaccagca ggatcatcga gaggggtttg gcgcttcagc ggaacccacc cccggtccac
     2821 cagaagcggc ggtgcgccgt cgcgatccaa cgggacgatc tggtcgaacc cgatcgtagg
     2881 gccggcacgg gtatcccgta cttcaacgcc aaactgtgcg gcgcgatcaa actggaaccg
     2941 gccggtgaca gagactttgg tgtacggcgc gggtgcatcc atcagcggca cggccggcgc
     3001 cgcttcagcc tttgcgatct gctcgaggat ggtctgtttc caatgcagcc gttgaacttg
     3061 ccacgtccca agtccgatca ggacgaccat catgacgagg gtgcttaaac cgggaacgac
     3121 aaaccggccg tagcctgccg tcacaggctt atctcggtac gacggcgacg gatcgcggcc
     3181 gccagaacag cgctcggagt catgataacc gtccattggt ggcatgcaac cgcggcgacg
     3241 gcaggaagaa aaacttcacc caaaacgcag ttccgactat cggcgccccc ggcacagcga
     3301 tgaccaaggc ggccccggta tcggatcgag ggtgtcgagg cgcggccggc tactccgcag
     3361 cgatctgccc cgcgccatac caatagatgc agatgaaaag aaacagccac accacatcga
     3421 caaaatgcca gtaccaggca gccgcctcga agccaaaatg gcgctccgga gtgaattggc
     3481 cgcccctcgc gcggaaccag cacacgatca ggaagcatgt cccgacgatc acgtggaagc
     3541 cgtggaagcc ggtggcaagg aagaacaccg acgaatagac gccaccgccg ttgaacttga
     3601 atggtgcgtt ggagtactcg atcgcttggc acgtcgtgaa ggccaggccg agcaggatgg
     3661 tgatgccgag gccgcggacc aagcccttgc ggtcgccttc cagcaggctg tggtgcgccc
     3721 aggttacggt cgtccctgac agcagcagga tcatggtgtt gagaaacggc aggtggaacg
     3781 gatcgaacgt gtggacattg gtcggcggcc agaccgtctg cccgtccccc tgagtctcgg
     3841 gaaacagaag gaagttgaag taggaccaga agaagccgac gaagaacgtg acctcgctgg
     3901 cgatgaacaa ggtcatgcca tagcgcaatc ccaggcgtac gatgacgctg tgggcaccag
     3961 gggtgcgcga ctcgcgaata acctgtcgcc accagaagaa catggtggtc agaacggtca
     4021 gcagtcccag aacgagtacc acgtagttcc cgtaatgggc tgccaggacg atgccaaaca
     4081 cggtcaggaa gccgcccagg gatccaatga tcggccacgg gctgggatcc ggtaaatgat
     4141 agggatgttt gataccagag gtagccaggc tcggtgcact gtgcgcgtcg ctgctcatgc
     4201 catacccatc gaaacatcgc cggggaagcc gccccggccc gatcggcgca taatcgccaa
     4261 aacccgcacc agttcaagtg ggcggaaaga cgtcaatttg ttcgcgattc ttgttcaggc
     4321 tccgccgccg aagtcccacc cacatgcggc cccgccttgg caagggcgcc ggacgccgcg
     4381 gcatcaccca aagagcggaa gaacgtgtag gacaaggtga tggtcttcac gtcggcggta
     4441 ttcggatcgg cacggatcgc cggatcgacc cagaagctga cgggaaattc catactttga
     4501 tcggccatca gcatctgttt gttgaagcag aagcatgccg tcttgtggaa gtatttccca
     4561 actttctccg gtgtgacgtt gtaaagcgcc atgcccgtca ccggctggct ggactggttc
     4621 ctggcgtgat agaaagcgac ctgttcatcg cccaggttaa ccgaaacctc gagctggtcc
     4681 ggcgcaaaaa tccacggcaa accagggttt gtgtccgcgt tgaagcgcac ccggatcgtc
     4741 ccgccgctgg agcccggggc gggagccgga ccgataccag gcgtgccggc atagccggtt
     4801 gccgcgcaga acaaccggta aaggggtatg gccgcgaacg acatacccac catgcccacc
     4861 accgccaaag ccacggtccc gccaagaagc gcgttgcgtt ggctcgcggt cattcagtag
     4921 tgcgccaatt tgacgatgga gaccgcatag aaaagggcac agagccccaa taggacgatc
     4981 aggatggccc agctccgtcc tcggcgccgg cgaatcgcat cgcggcccac gtctgcttcc
     5041 ggaggagagt ctgcttccga aggagagtct ggcccggcca ccggcctatc ctgccagtcg
     5101 gtcgatagcc aacgccccaa gcaacgcgaa caggtataag acggaatact tgaaagcagc
     5161 cttcgctgga acatccccgg tcaggctatt gccggcagca tcctgccgat cggtcagcac
     5221 ctgatacacg cgctgcaaaa acaccacccc gagcaaacct gccgtcaggc catagggaag
     5281 gccactgaat cccatgaaaa atggcacaag cgtcagagga acgagaacca gcgtataaag
     5341 caggatttgc cggcgggttt ccttcgcgcc cgcgaccacc ggcagcatgg ggattccggc
     5401 gcggcggtaa tcgtcatttg cccatagcgc cagcgaccag aaatgcggcg gcgtccagac
     5461 gaacacgatg gcgaacagca cgagcgggat cacatcgacc gacccggtta ccgctgccca
     5521 gccgatcacc ggcggaaaag cgcctgccgc gccgccaatg acgatgttct gcggcgtgcg
     5581 ccgcttcagc cagaccgtat aaacaaagac gtagaagcaa atcgacaacg caagaatcag
     5641 cgccgccgga atgttgagcg ccaagcccat catcagcacg gagccaaccg ccaacgtcac
     5701 tccaaatccc aacgcgggac caggttggat ccggccagcc gggataggtc ggttacgggt
     5761 acggcgcatg atcgcgtcga tatccctgtc gtaccacatg ttgatcgcgc cgcaggcgcc
     5821 cgcggcaatc gcgatgcaca ggatcgcggt aaacgccagg acaggatgaa ggtggccggg
     5881 tgccaccgcc aatccaatcg ctccggagaa gaccacgagc gtcaggaccc gtggcttcaa
     5941 caacgcgatc cagtcgcggt agtccgattc gagacaaagg tcgcgggtcg gcccaagcgc
     6001 cgccgccaga ggggggctgt ccccctcgtt ggcgtggatc atcgaaggcg tcgcatcaat
     6061 cactgactag gggatccgcg gcatttccag gtaggtgtgg aaggcaggtg gtgaagtctg
     6121 ggtccattcg agcgtcgtcg caccctgacc ccagtaattg tccgccacat gctccttgga
     6181 agtaaaggta cgaaaacaaa cgtacaggaa caccaaaaac gcaatgccgc tgatataggc
     6241 cccgatggac gacacgaagt tccaaccggc gaaggcttcg ggatagtcgg gatagcgccg
     6301 aggcataccc gacaggccga ggaaatgctg cgggaagaag gtcagattga cgccgatgaa
     6361 ggtcagccag aagtgcacct taccccagaa ctccgggtac tggtggccag acatcttgcc
     6421 gatccagtag tagaatccgg caaagatcga gaaaaccgca cccagcgaca gcacatagtg
     6481 gaagtgcgca atcacatagt acgtgttgtg cacgacctgg tcgatgccgg cattggcgag
     6541 caccacgccg gtgacaccgc cgatggtgaa cacgaagata aagccgatcg cccacagcat
     6601 cggggtcttc agttcaatcg agccgcccca catcgttgcg atccatgaaa aaatcttgat
     6661 gccggtcggt accgcgatca ccagggtcgc ggccatgaaa tacgccctcg tgtcaacgtc
     6721 gatgccggac acgaacatat ggtgcgccca tacgacgaag ccgaccacgc cgatggcgat
     6781 catggcatag accatgccca ggtaaccaaa aatcggcttt ttgctgaagg tggatacgac
     6841 gtggctgatg atcccgaagc caggcaggat catgatgtaa acttccggat ggccgaagaa
     6901 ccagaacaga tgctggaaca gcaccggatc gccgccgccc gtagggtcga agaaagcggt
     6961 gccgaagttt cggtcgcaga tcagcatggt gatcgcaccc gccagcaccg gaaccgcgag
     7021 caccagcaga aacgccgtca ccaactcgga ccagatgaac agcggcatct tgtgcagggt
     7081 catgcccggg gcgcgcatgt tgaagatcgt cacgatgaag ttcatcgcgc ccagcagcga
     7141 actcacgccg gcaaggtgca acgcgaacag ggtgcaatcc atccccatgc cgttctcgta
     7201 cgtggcgttc gacagcggcg gatacagcgt ccagcctgac ccggactcgt tcatcacaag
     7261 accgatacag atcagcacga aggcgggcgg cagcagccag aagctgatgt tgttgagcct
     7321 cgggaacgcc atgtccggcg cgccgatcat cagcgggata aaccagttgc caaaaccgcc
     7381 gatcagtgcg ggcatgacag agaagaagat catcagcagc ccgtggctgg tgatgatggt
     7441 attccactcc tggccgtcag ggacgattgt gttgtgcgga aattgcaact gcgcccgcat
     7501 gatctgggac agcaccccgc ccacgaga
//
LOCUS       DASWQN010000821         9148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1663664, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000821 DASWQN010000000
VERSION     DASWQN010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9148
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663664"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VGC82_13870"
     CDS             complement(<1..242)
                     /locus_tag="VGC82_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY0424405.1"
                     /translation="MFLGLIVPSAAWVQPAVARSAPDSFADLATKLLPAVVNVSSTQT
                     ITAKNGGPGPGAGPEMPVFPPGSPFEQFFKDFLNRNR"
     gene            complement(563..1444)
                     /locus_tag="VGC82_13875"
     CDS             complement(563..1444)
                     /locus_tag="VGC82_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY0424406.1"
                     /translation="MSRFAIAGIAVLVVLAFILNASLFTVAQTEQVLVVQFGEVVRPI
                     EQPGLHVKLPIVQNVISFDRRLMAVELPGEEVILGDQRRLIVDSVTVFRISDPLRFYQ
                     AIGPIPDNIRGRLNSIVTGSLRRVLGNNKLLDVLSADRERIMATIRGQVNTEMQGFGV
                     TIADVRIRRADLPQENTQAILSRMQSERQRVAAQARAEGAEASQRIKADAERERTVLV
                     ADARSTADRLRGEGEGEATKIFAQSFGQDTAFFSIWRTLQGYRDIFEAGAARLVVTPD
                     NDYLRYLQAPPPDVSRP"
     gene            complement(1441..2772)
                     /gene="hflK"
                     /locus_tag="VGC82_13880"
     CDS             complement(1441..2772)
                     /gene="hflK"
                     /locus_tag="VGC82_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY0424407.1"
                     /translation="MPWNNGGPGPWGNPPGSSGGDDDKKPTRGPWGNPGGNKGTKGGD
                     GEERPDPDRGPRRPGGPFGGGGPFGGGGGPFGGGGAAPDLDRLIAQAQAYIRGMMGGG
                     SGGGRSGGPLRGFANSRGLILLLLVVVALWVGSGFYRVQPDEQGVVMRFGAYSYWTPP
                     GLHWHVPWPVESVSQPTVTRINRTEIGFRSGAAGQIDSGRDSAGRDVLAESLMLTGDE
                     NIIDIDVAVFWRINDASAFLFNTADPQALVRAVAESSMREVIGHTPIQPALTQLRAQI
                     ESDVHKQTQEILDRYKAGVEVTQVQLQKVDPPAAVIESFRDVQRANTDAERMRNEAES
                     YRNDIVPRARGDAARIVAEGQGSKAASVAQATGQAQRFESVLKAYQSAKEITLRRMYL
                     DTMQDVLAHSQPLVIDDKLKGLVPFLPLNVPPPTVVAPVGPASSAAVGASR"
     gene            2916..4076
                     /locus_tag="VGC82_13885"
     CDS             2916..4076
                     /locus_tag="VGC82_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY0424408.1"
                     /translation="MVDASPDALRAALRAIKDPASSRDIVTAGLVEGIEVRGGLVQAT
                     LLTDRAHAAAMEPVRREVEAVLMRQPNVTNATAILTAHKAPGGPKQAAPVGAAGQGHG
                     QSQSQGQSHGGPGGKPALLLPDVKAIVAVASGKGGVGKSTVAVNLAVCLARQGHKVGL
                     LDADIYGPSLPRMTGLNRKPEVRDGKMIPLQAWGLSCMSIGFLVDEETPMIWRGPMVM
                     GALEQMMGQVEWGPLDILVVDMPPGTGDAQLTMAQRVALTGAVIVSTPQDIALIDARR
                     GVRMFEKTKVPVLGLVENMSFYCCPNCGHRADIFGHGGARTEAARLGAEFLGEIPLLL
                     DIRLASDGGTPISALAPESDAAKAFAALAARVWEKIGGTTAERSHGPRIVID"
     gene            complement(4093..6303)
                     /locus_tag="VGC82_13890"
     CDS             complement(4093..6303)
                     /locus_tag="VGC82_13890"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEY0424409.1"
                     /translation="MRRNPHLFRLTANVALAALLWDSVLPASTLAQPPPPPLPQPYQV
                     QPAQPQGDPPSRVGRIAATSGTVSFHNQGDTQWSRASVNYPVSSGNAFWTEPAAEAQL
                     EIAANRIVMAGTTEFDIATLDANGIQAVAAQGEVYIHLNDLAADEAWTIQTPRGLVRL
                     EGVGRYGIVVGNTEQPTLVTVIDGSAQIDGPGVSLHVGNGETASIGGTDTFEGNVGPA
                     RNDAFLSARQDAERPRPAPAAPMPAVPMPAAPPVQVPPQVAAMPGGSDLNRYGDWSEA
                     PEYGRVWYPPVAQGWVPYRHGHWAYVAPWGWTWIDDAPWGFAPFHYGRWIEIGGRWAW
                     TPGGYAAAAPPVYAPALVTFIGIGAGVALGAALASGSIGWVPLGPREPFRPWYRASDR
                     YTREVNIQHVTNINNNNSNVTINNYVNRGAATAIPSAAMAASRPVQSVAQPVSAQQFA
                     TARPFVGQQPVRPTAATAGVTPAVARHMNLGPAAGFRPAPGPAVHLQAPGPTVVRTAV
                     PGAAVPVARVPATAVSPAIAPGAAVRGATVPGSIPSQLARPMVVAPGQVQPGAVPAAT
                     PAAIGNPAAQPNRPAATTQAPASPAASSGAISAPQTGRATQAAPTVVRPGEPRSAPSA
                     NAAFAHPGQPAPPVHSEVRPLPQVIAPSRPEAPRIAVPAPRPEPQHFAPAAPPPHVAT
                     PNIVHTEPPHFAPQPMAMRPAPPPAPMPRAAAPAPAPVPAPQAPAAHQKRPGER"
     gene            6826..7653
                     /locus_tag="VGC82_13895"
     CDS             6826..7653
                     /locus_tag="VGC82_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY0424410.1"
                     /translation="MPAAIIVAAVIVFPWIFTLFMSVHDWKVSGATPFVGMANYAKML
                     VDDRFQWAIVRTLTFTAGSVAAPVLLGVWAAVCFASNFRFKGLARTIFVLPMMATPVA
                     ISLVWTMMFHPQLGVLNYILSLVGLPPSAWVYDSATVIPTLVMVETWQWTPLVMLIVL
                     GGIASLPTDPYEAAVLDGASTWQMFRHITLPLAWPFIVVAAVIRMIDALKAFDTIYVI
                     TLGGPGTSSETLNILLYQTAFAYYDLGYGSAMVVIFFVLILLISMLLLHVRQKQAWQ"
     gene            7662..8495
                     /locus_tag="VGC82_13900"
     CDS             7662..8495
                     /locus_tag="VGC82_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0424411.1"
                     /translation="MARHRFKRLLGRAGLYFSVLMLISPAVLFFLWMLSLAFKNEADN
                     TAFPPVFIPNPPTFANFVDVFEKNDFLTYTINSVIVSFSATGLALLFGVPAGYGIAKA
                     KAGKAAVLILIARVTPGLSYLIPLFLLFQWMGLIGTLAPLVITHLVITVPIVVWIMIG
                     FFEGLPNELEEAALVDGATIWQAFRYVAMPLARPGITVAMILAFIFSWNNFIFGVVLA
                     GRSTRTLPVAVYNVLTFEQISWGPLAAAALIVTAPVLLLTLLMQKEIVAGLTAGGVKG
                     G"
     gene            complement(8533..8910)
                     /locus_tag="VGC82_13905"
     CDS             complement(8533..8910)
                     /locus_tag="VGC82_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0424412.1"
                     /translation="MATPPNPVSDGQVGLLRDTIVALVRRDGADLSARQLAVFLTCYL
                     TDGAHTVRGLAAELSVSKPAITRALDRLGELDLARRKVDPLDRRSVLVQRTPRGSAFL
                     RDLRSIMTEVSAGGRKAPKKAAA"
BASE COUNT         1565 a         2810 c         3057 g         1716 t
ORIGIN      
        1 cggttgcgat tgaggaagtc cttgaagaat tgctcgaacg gggagcccgg cgggaacacc
       61 ggcatttccg gaccggcacc aggacccggt ccgccgttct tcgctgtaat ggtctgagta
      121 gacgacacat tcaccaccgc aggcagcaac ttggtagcaa gatcagcgaa actgtcgggt
      181 gccgatcgcg cgacggcggg ttgaacccat gcggcagacg ggacaatcaa gcccaggaac
      241 atgccgaaca aggcggaacg gacaagcgtg gaacagtgca ggcgcatgat cggttttccc
      301 tggcgagtta gccgcatatg gtccggttcc tcaggctagc aacttttccg gcgaaacaca
      361 ccggtcgtgt tgtgcgattt ccgatcgtga ccatcatcgc gtcatggtgt cctgtccccg
      421 aagttcatcg catgatgcga cgggcacagg ggataagacg gcccgaaccg ccggcgtcct
      481 ggaccggagg tttgctgtcc tggatcggag gtttgcccga agttgcaagc ttctgaacca
      541 ggacgataga tcggcgcgaa gttcaggggc gtgagacatc gggtgggggc gcctgcaggt
      601 atcgcagata atcgttgtcc ggcgtgacca cgagacgggc ggcgccagcc tcgaagatgt
      661 cgcgatagcc ctgcaacgtg cgccagatcg agaagaacgc cgtatcctgg ccgaacgact
      721 gggcaaaaat ctttgtcgcc tcgccctcgc cttcgccacg cagccggtcg gcggtggagc
      781 gggcatcggc gaccagcacg gtgcgctcgc gttcggcatc ggccttgatg cgttggctcg
      841 cttcggcacc ctcggcgcgc gcctgggcgg caacgcgctg gcgttccgat tgcatccgcg
      901 acaggatcgc ctgggtgttt tcctgtggca gatccgctct gcggatacgc acgtcggcga
      961 tggtgacgcc gaagccttgc atctcggtgt tcacctgccc cctgatggtg gccatgatcc
     1021 gctcccggtc ggctgacagc acgtccagca gtttgttgtt acccagaacc cgccgcaggc
     1081 tgccggtgac gatggagttc agacgcccac ggatattgtc cgggataggc ccgatcgcct
     1141 ggtagaagcg cagcgggtcg gaaatgcgga acacggtcac gctgtcaacg atcagccggc
     1201 gctggtcgcc cagaataacc tcctcgccgg gcagttcgac cgccatcagg cggcggtcga
     1261 agctgatgac gttctgcacg atcggcagtt tgacgtgcaa gccgggttgc tcgatcggcc
     1321 gcacgacttc gccgaactgc acaaccagaa cctgttccgt ctgcgcgacg gtaaacaggc
     1381 tggcgttcag gataaaggcc agaaccacga ggacagcgat gccggcaatg gcgaagcggc
     1441 tcatcggctg gctcccacgg ctgcggagga ggcggggcca accggtgcga ccacggtcgg
     1501 cggcggcacg ttcaacggca ggaacggcac cagccccttc agcttgtcgt cgatcaccaa
     1561 tggctgggaa tgggcgagca cgtcttgcat ggtgtccaga tacatgcgcc gcagggtaat
     1621 ctccttggcc gactgataag ccttcagcac gctttcgaag cgctgcgcct ggccggttgc
     1681 ctgggcgacc gacgcggctt tggaaccttg cccctcggcg acgatcctgg cggcatcgcc
     1741 gcgggcacgc ggcacgatat cgttgcggta ggactcggcc tcgttgcgca tccgctcagc
     1801 gtcggtattg gcgcgctgca cgtcgcggaa actttcgatc accgcggccg gcggatcgac
     1861 cttttgcaat tgcacctggg tcacttcgac gccggccttg taacggtcca ggatttcctg
     1921 ggtttgcttg tgcacgtcgc tctcgatctg ggcgcgcaac tgggtcagcg ccggctgaat
     1981 cggagtgtgc ccgatgacct cgcgcatgga gctttcagca accgctcgta ccagcgcctg
     2041 cgggtccgcc gtattgaaca ggaacgccga agcgtcgttg atccgccaga acacggcgac
     2101 gtcgatgtcg ataatgttct cgtcgcccgt cagcatgagg ctttcggcca gcacgtcgcg
     2161 cccggccgag tcgcggccgg agtctatttg tccagccgcc ccggagcgga agccgatttc
     2221 ggtccggttg atgcgggtga cagtcggctg cgagaccgat tcgaccggcc atggcacatg
     2281 ccaatgcaac cccggcgggg tccagtagga atacgcgccg aaacgcatca cgacgccctg
     2341 ctcgtccggc tgcacccggt aaaagccgct gccgacccag agcgcgacga cgaccagcaa
     2401 cagcaggatc aggccgcggc tgttggcgaa cccccgcagc ggaccgccgc tgcggccgcc
     2461 gcccgaaccg ccacccatca tgccgcggat gtaggcctga gcctgagcga tcagccggtc
     2521 gaggtccggt gcggcgccgc caccgccgaa ggggccgccg ccgccgccga aaggaccgcc
     2581 tccgccgaac gggccccctg ggcggcgagg tccgcggtcc gggtcgggac gttcttcgcc
     2641 atcaccacct ttagtaccct tgttgccgcc cgggttcccc caggggcctc gtgtgggctt
     2701 cttgtcatcg tctccgcccg atgagcctgg cgggttgccc cagggaccgg gaccgccgtt
     2761 gttccaaggc atttttaggt ggctcctgtc gaatcgccga atgcgcgagc cgttggcgga
     2821 aacggtgtca tacgccatat caatatattc aagcgctcgc ggcgcggtgc ttatccgccg
     2881 gccgtgtcag ttttgcaagg tgaggttcaa tatccatggt cgatgcttct ccggatgccc
     2941 tgcgtgcggc tttgcgcgcc atcaaggacc cggcctccag ccgggatatt gtaaccgctg
     3001 ggctggtgga aggaatcgag gttcgcggcg gcctggtgca ggcgaccctg ttgaccgacc
     3061 gtgcccatgc ggcggcgatg gagccggtgc ggcgagaggt cgaagccgtg ctgatgcgcc
     3121 agccaaacgt gaccaacgca accgcgatcc tgaccgcaca caaagctccg ggggggccga
     3181 aacaggcggc accggttggg gcggcgggcc aaggccatgg ccagagccag agccagggcc
     3241 agagccacgg gggcccaggc ggaaagccgg cgctattgtt gccggacgtc aaggccattg
     3301 tcgcggtcgc atccggcaag ggtggcgtcg ggaagtccac ggtcgcggtc aacctggcgg
     3361 tctgcctggc caggcagggt cacaaggtcg gactgctgga tgccgatatc tacggaccca
     3421 gcctgccgcg gatgacgggg ctgaaccgca agccggaggt tcgcgacggc aagatgatcc
     3481 cgctgcaggc ctggggcttg tcctgcatgt cgatcggctt tctggtcgat gaggaaaccc
     3541 cgatgatctg gcgtggcccg atggtgatgg gcgcgctgga gcagatgatg gggcaggtcg
     3601 agtggggccc gctcgacatc ctggtggtag atatgccgcc gggaaccgga gacgcgcaat
     3661 tgaccatggc gcagcgagtg gcgctgaccg gcgcggtcat cgtctccacg ccgcaggaca
     3721 tcgcgctgat cgacgctcgg cggggcgtcc ggatgttcga aaagaccaaa gtgccggtgc
     3781 tcgggctggt cgagaacatg agcttctact gctgcccgaa ctgcggtcac cgcgccgaca
     3841 tttttggcca cggcggcgcg agaaccgaag ctgctcgact gggggcggag tttttaggcg
     3901 aaatcccgct gctgctcgat attcggctcg cgtccgatgg cggcacgccg atctcggcct
     3961 tggcgcccga aagcgatgcg gcgaaggcat ttgccgccct ggccgcccgt gtgtgggaga
     4021 agattggcgg caccactgcg gaacgcagcc acgggccgcg gatcgttatc gactgacggc
     4081 ggcgccagcg ttctaccttt ccccagggcg cttctggtgg gcggcgggcg cttggggagc
     4141 aggcaccgga gcaggcgctg gagcagccgc acggggcatc ggcgccggag gtggtgccgg
     4201 ccgcattgcc atcggttgcg gtgcgaagtg cggtggttcg gtatgcacga tgttcggagt
     4261 agcgacatgt ggcgggggtg cggccggggc gaaatgctgc ggctctgggc gtggggccgg
     4321 cacggcgatg cgcggggctt cggggcgcga gggcgcgatc acctgcggca atggccgcac
     4381 ttccgaatgg acgggcggtg ccggctggcc tggatgcgcg aacgcagcat tggcacttgg
     4441 cgcagaccgt ggctcgcccg gtcgcacgac cgtcggcgca gcctgcgtcg cccttccggt
     4501 ttggggtgct gaaatggcgc ctgaactggc cgccggcgaa gctggggcct gcgtcgtggc
     4561 agcagggcgg tttggttgcg cggccgggtt gccaatggca gcaggcgtgg cggcaggcac
     4621 ggcgccaggc tggacctgcc ccggtgcaac gaccatcgga cgagcgagct gactggggat
     4681 tgacccgggc accgtggctc ctcggaccgc ggctccgggt gctattgccg gggaaaccgc
     4741 tgttgccggg actcttgcca ccggaacggc ggctccgggg actgctgttc ggacaacggt
     4801 tggcccgggc gcctggaggt gaaccgccgg accgggtgcg ggccgaaaac ccgccgccgg
     4861 gccaaggttc atgtggcgcg cgacggctgg ggttacgccg gccgtcgcgg ccgtgggacg
     4921 gactggttgt tggcccacaa acggacgcgc cgttgcgaat tgttgcgccg aaaccggttg
     4981 agcgaccgat tgaaccggac gcgatgctgc catggcggca ctggggatcg ctgtggcggc
     5041 acctcggttg acgtaattat tgatggtgac gttgctgttg ttgttgttga tgttcgtgac
     5101 atgctggatg ttgacctcgc gcgtgtagcg gtcggaggct cgataccagg gacggaacgg
     5161 ttcgcgtggg cccagcggca cccagccgat cgatcccgat gccagggccg cgccaagggc
     5221 gacgccggcg ccaataccga tgaaggtgac caaggccggc gcgtaaaccg gcggggccgc
     5281 agcggcatag ccgcccggcg tccaggccca acgcccgccg atctctatcc agcggccgta
     5341 atggaacggc gcaaaccccc acggcgcgtc gtcgatccag gtccagcccc aaggcgccac
     5401 ataagcccaa tggccgtggc gatacggcac ccatccttgc gccaccggcg gataccagac
     5461 gcggccgtat tccggcgctt ccgaccagtc gccataccga ttcaggtcac tcccacccgg
     5521 catggcggcg acctgcggag ggacctgcac cggaggggcc gctggcattg gtaccgctgg
     5581 cattggcgcc gctggggctg gccgcggccg ctcggcgtct tgccttgcac tcaggaacgc
     5641 atcgtttcgc gccggtccga cattgccctc gaaggtatcg gtgccgccga tcgaggcagt
     5701 ttcaccgttg ccgacgtgca gcgatacgcc gggtccatct atctgcgccg aaccgtcgat
     5761 cacagtgacc aaggtcggct gctcggtgtt gccgacgacg atgccgtagc gacctacgcc
     5821 ttcgaggcgc accaatccgc gaggggtctg gattgtccag gcctcatcgg cggcaaggtc
     5881 attcagatgg atataaacct cgccctgtgc cgcgaccgcc tgaattccat tggcgtcaag
     5941 cgtggcgata tcgaactcgg tggtacccgc catgacaatg cggttggcgg cgatctcaag
     6001 ctgcgcttcg gccgcgggtt cagtccagaa ggcgttgccg ctgctgactg gatagttgac
     6061 gctggcccgg ctccactgcg tgtcgccctg gttatggaac gacaccgtgc ctgaagtggc
     6121 ggcgatgcga ccgacgcggg aaggtggatc gccctggggc tgcgccggct ggacctgata
     6181 cggttgcggc aggggtggcg gaggcggctg tgccagggtg gaagccggca gaacggaatc
     6241 ccacagcaag gcagccagcg ccacattggc tgtcaggcgg aaaaggtgag gatttctgcg
     6301 catcctattg gtccgatcgc tgttggtcgt agccggagga cgatccggga aggcggcgaa
     6361 attcacggat cacccgtggc gggcgtccga tacccgatct tcctgtttcc ggcgtggaag
     6421 tgccgcgcct cctttgttga agctgttgaa gcgccgcgtc gccgttcacg ctgatgttaa
     6481 ccgatgcgcg gaccccgatc caacaatcct gtttggcggc gactgccgtt ggctgcttca
     6541 agccgcccgg atttgcatcg aaggaccttg gtcccgcggc gcaagcccgc cggtccggac
     6601 aacgagatgc tcctcgccat ccggacctca ggtcgatccg ctgtcccgcg gtggtgtgga
     6661 ccggtcgcct aagctattga gggtgcgcaa ggggctgtcc aacgccgggc cccgcaacca
     6721 gagacctaca accgcatgag cgcatccctt tctcaggcca gccgggtgtc gccggaccga
     6781 gcgcaacatc gccacaacta tgctcgccgc tactggatat ttgccatgcc agcggcgatt
     6841 atcgttgcgg cggttattgt gtttccgtgg atattcaccc tgttcatgtc ggtgcatgat
     6901 tggaaggtat cgggtgcgac gccgttcgtc ggcatggcga actacgccaa gatgctcgtg
     6961 gacgatcgtt tccaatgggc aatcgtgcgt acattgacgt tcaccgcggg ttcggtcgcg
     7021 gcgcctgtac tgctgggcgt gtgggcggcg gtctgcttcg cctccaactt ccgcttcaaa
     7081 gggttggcac ggaccatctt cgtgctgccg atgatggcga cacccgtcgc gatctcgctg
     7141 gtatggacaa tgatgtttca cccgcaactg ggggtgttga actatatcct gagcctggtt
     7201 gggctgccgc cctcggcctg ggtctatgac agcgcaacgg tcatcccgac ccttgtgatg
     7261 gtggagacgt ggcagtggac gccgctggtg atgctgatcg ttcttggcgg gatcgccagc
     7321 ctgccgaccg acccatacga ggccgcggtt ttggacggcg caagcacctg gcagatgttt
     7381 cgccatatca ccttgccgct ggcttggccg ttcatcgtgg tagccgcggt catccgcatg
     7441 atcgacgcct tgaaggcgtt tgacacgatt tacgtgatca ccctgggggg tcccggcacg
     7501 tcgagcgaga cgttgaatat cctgctgtat cagacggctt tcgcctacta cgacctgggc
     7561 tacgggtcgg cgatggtggt catcttcttc gtgctgatcc tgctgatcag catgcttctg
     7621 ttgcacgttc ggcaaaagca ggcatggcaa tgaaacgcag catggcacgg caccgtttca
     7681 aacgcctgtt gggacgggcc gggttgtact tctcggtgct gatgctgatc tcgcccgcgg
     7741 tcctattttt cctttggatg ttgtcgctgg cgtttaagaa cgaagccgac aacaccgcat
     7801 ttccgccggt gttcattccg aacccgccga cgttcgccaa ctttgtcgac gtcttcgaaa
     7861 agaacgactt cctgacctac accatcaatt cggtgatcgt gtcgttcagt gccaccgggc
     7921 tggcgctgct gttcggcgtg ccggcgggat acggcatcgc caaggcaaag gccggcaagg
     7981 cggccgtgtt gatcctgatc gccagggtaa cccctggcct gtcctatcta attccgttgt
     8041 tcctgctgtt tcagtggatg ggtctgatcg gcacgctggc accgctggtc atcacccatc
     8101 tggtgatcac ggtcccgatc gtggtctgga tcatgatcgg cttcttcgag ggcctgccga
     8161 acgaactgga ggaagcggcg ctggtcgatg gtgctacgat ctggcaagca ttccgctatg
     8221 tcgcgatgcc gctggctcga cctgggatta ccgtagcgat gatcctggca tttatattca
     8281 gttggaacaa cttcatcttc ggggtggttc tcgcaggccg ttcgacgcgg acgctgccgg
     8341 tcgcggtgta taacgtgctg accttcgagc agataagttg gggtccgctt gccgcggcgg
     8401 ctctgatcgt gacggcgcct gtcctgttgc tgaccttgct catgcagaag gagatcgtcg
     8461 cggggctgac cgcgggcggc gtaaagggag gatgaacccc ttcaacgcca tacgccagcc
     8521 gccacagctt gatcaagccg cggctttctt cggtgctttt ctcccgccgg cggacacctc
     8581 ggtcatgatg cttcgcagat cacgcagaaa cgcagaaccc ctgggcgtgc gctgcaccag
     8641 cacactgcga cgatcgaggg gatcgacttt gcgccgcgcg agatcgagtt caccaagacg
     8701 atccaaagcc cgggtaatgg caggcttcga cacgcttagc tcagctgcca ggccgcgaac
     8761 cgtgtgggca ccatcggtca aatagcaggt caaaaagacc gcgagctggc gggcggaaag
     8821 atcggcacca tctcggcgca ccagcgcgac gatcgtgtcc cggaggaggc ccacctggcc
     8881 gtccgaaacg gggtttggag gagttgccac gtcattaatc ccttcctagg cgggttacgg
     8941 atcacgtccc cacatttcat ttcctaggta gtaaatagat tgtggccaat cttattacgt
     9001 atattcaact cataacaact tggtgacact tcctcatgat cgctcaggca taatcagttc
     9061 gcgggccggt cattcagcaa tgaaacgact tcgatatcgt cttcaggcac gcagcgacag
     9121 cttgcgcgca ggcatcaacg acctcgag
//
LOCUS       DASWQN010000822         1740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1268120, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000822 DASWQN010000000
VERSION     DASWQN010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268120"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VGC82_13910"
     CDS             <1..381
                     /locus_tag="VGC82_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424413.1"
                     /translation="SVEAGGMRGVVEASLANSYPPVVRHDSATFAAFRARWLGNDPES
                     FAAINRMLAAENIEHELAAIDCLVLLTAGRHDGLRPPSIIEPMSRQIPGARFLELNSG
                     HFASVQTPGLVAQAIHYFLHGLGL"
     gene            complement(626..>1740)
                     /locus_tag="VGC82_13915"
     CDS             complement(626..>1740)
                     /locus_tag="VGC82_13915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0424414.1"
                     /translation="ALDENPILALERDMELLQHLDALNYSEAWIGEHHSGGFEIIASP
                     ELFIAAAAERTRHIRLGTGVVSLPYHNPFIVADRMVQLDYQTRGRAMLGVGPGSLVHD
                     AIKIGIDPADQRRMMNESLDVIMDLFRGESVTRKSDWFDLRDAALQLPPYTRPRMEMA
                     VAAARSPAGALAAGRHGLGMLSIGGTSDDALKHHASNWDLYADTAKANGHVPDRKNWR
                     LVTLMHVAETREKARNNVRHGLDKFCDYFRDVATFPIVPGDITDRYQYMVDSGTACIG
                     TPDDAIVFIERLLKGSGGFGVIMELAQNWADWADTKRHYELMARYVHPHFQASREWRS
                     TSYDYARDHHDDFTGRSGAAVQAEIDRLAAKRRAAE"
BASE COUNT          299 a          549 c          557 g          335 t
ORIGIN      
        1 tcggtggaag ccggcgggat gcggggggtt gtggaagcca gcctggccaa ctcctatccg
       61 ccggtcgtta ggcacgattc agcgacattc gccgcattcc gagcgcgatg gctcggcaac
      121 gatccggaaa gcttcgccgc gatcaaccgg atgctggctg cggaaaatat cgagcacgaa
      181 ctcgccgcga tcgactgcct ggtgctgttg accgccggcc ggcatgatgg gctgcgacct
      241 ccgtcgatca tcgagccgat gtccaggcaa attcccggcg cgcggttcct ggagttgaac
      301 agcggccatt tcgcatcggt gcaaaccccg ggtctggtcg cgcaggcaat ccattacttc
      361 ctgcacggcc tggggcttta gagcagatcg ccagaaagcc gaaaggtccg aagcgtgagt
      421 ctgcctggag agcgatcgac cagcgcggtt ttacggtgac tgggaaaaga gttctctcgt
      481 ggttgcaaag aaagtcgtgg gtggccgcgc caagcacggc catgacatat cggcgccagg
      541 cggcgagctg gcttgaacct tgttcagtca gcgtaaaacc gctttagcgc gtcgcgatcc
      601 tgatcggacc gcggtgcgcg atagcttatt ccgccgcgcg ccgttttgcc gccagccggt
      661 cgatttccgc ctgcaccgcg gcgcctgacc gtccggtgaa gtcgtcgtgg tggtcgcgtg
      721 cataatcgta gctcgtgctc cgccattcgc gcgacgcctg gaaatgcgga tggacgtaac
      781 gggccatcag ttcgtagtgc cgcttggtgt ctgcccagtc ggcccagttc tgcgccagtt
      841 ccatgattac gccgaaaccg ccggagccct tcagcagccg ttcgatgaat acgatcgcat
      901 catcgggcgt gccgatgcag gcggtgccgc tatccaccat gtactggtag cgatcggtaa
      961 tatcgccggg gacgatcggg aaggtcgcga catcgcggaa gtaatcgcaa aacttgtcca
     1021 atccgtgccg aacgttgttt ctggccttct cgcgggtttc ggcgacgtgc atcagcgtca
     1081 ccaggcgcca gttcttgcgg tccggcacat ggccgttggc cttggccgtg tcggcataaa
     1141 ggtcccagtt gctggcgtgg tgtttcaacg cgtcgtcgct ggtgccgccg atcgacagca
     1201 tgcccaaccc atgccgcccg gccgccaggg caccggccgg agagcgtgcc gctgccaccg
     1261 ccatctccat ccgcgggcgc gtgtaggggg gcagttgcag cgccgcgtcg cgcaggtcga
     1321 accagtcgga cttgcgggtg acgctctcgc cgcggaacag gtccatgatg acgtccagcg
     1381 actcgttcat catgcggcgc tgatcggcgg ggtcgatgcc gatcttgatt gcgtcgtgca
     1441 ccagcgatcc gggcccgacg cccagcatcg cccgcccgcg ggtctggtaa tccagttgca
     1501 ccatccggtc cgcgacaatg aacggattgt ggtagggcag cgacactact cccgtcccca
     1561 gccggatatg ccgggtccgc tccgctgccg ccgcaataaa cagctccggg ctggcgatga
     1621 tctcgaaccc gccggaatgg tgttcgccga tccacgcctc actgtagttc agcgcgtcga
     1681 ggtgctgcag caactccata tcccgctcca gcgcgagaat ggggttttcg tccaaggcat
//
LOCUS       DASWQN010000823         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1531190, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000823 DASWQN010000000
VERSION     DASWQN010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531190"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VGC82_13920"
     CDS             <1..769
                     /locus_tag="VGC82_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0424415.1"
                     /translation="RGFDHDIREAMVETLEAQGIILHPNSTPEKLEASGDRRTLTLGS
                     GGTITTDLVFFATGRRANISGLGLDKVGIELNAKNAIKVDENLRTTQPHIDAMGDVTD
                     RVNLTPVATAEGHALADTLFGDNPRTVALWNIPSAVFTTPPVGIVGLSEEQACTLAPV
                     DIYLTRFTPMRHNLSGRGRKTTMKLVVDKATQKIVGAHMLGEDAPEIIQGIAIAVVMG
                     ATKADFDRTIGIHPTAAEEFVTLRTLTRTTALAEAAE"
     gene            847..2421
                     /locus_tag="VGC82_13925"
     CDS             847..2421
                     /locus_tag="VGC82_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424416.1"
                     /translation="MKRRSFLAAAAATALARPAVANGTKPLIFVPQANLTSLDPVWTT
                     ATVTRNFALMVYDMLYGRDENFVPRPQMVEGHVIDDDGKRWTMKLRDGLMWHDGTPVL
                     ARDCIASLQRWMKRDAAGVTINLRVDAIEAPDDRTLVWRLRKPFPLLPFFLSKVQPQP
                     VMMPERIAATVDPFKPVTEIVGSGPFRFLAKEYVSGAFAAFEKFDRYVPRNEPASYTA
                     GGHHVLVDRVEWRVIPDSATAANALTAGEVDWLELPQPDLLPMLKQKEGVTTGLLDLY
                     GTVGILRPNHLNAPTSNVGVRRAMFAAIDQREIMLATMGEDPSTWRAPMGYFLPNSKS
                     ANDAGMDFVRKRHSTDEVKRMLDEAKYGGEKIVFLHPTDQLAYNAFSTVVVDAFHKVG
                     LNVDEQMTDWGTVVQRRPSKEPLDKGGWSMFPAGAPGPEFVDPLLANTLRSNGSKAWF
                     GWPDDPVLEAAYEAWIDAPDEAERHKQATAFQAAAFQSVPSIPLGQYLPHAAWRSNIT
                     GLVKGSAPVFWGAKKE"
     gene            2428..>2712
                     /locus_tag="VGC82_13930"
     CDS             2428..>2712
                     /locus_tag="VGC82_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424417.1"
                     /translation="MAEGDLPGEPDANTLIRGFEATDATAVRDLFVLVNRLLSPPHLR
                     TAFEAYIARSLSEEIGRIPAYYAEHAGGFWVACRGEDVVGMFGLEAAGPNA"
BASE COUNT          519 a          861 c          878 g          454 t
ORIGIN      
        1 gcgcgggttc gaccacgaca tccgcgaagc gatggtcgag acactggagg cgcagggcat
       61 cattctgcat ccaaacagca cgcccgaaaa gctggaggcc agcggcgatc gtcgcacgct
      121 gaccctgggc agcggcggga cgataacgac cgacctggtg ttcttcgcca ccggccgccg
      181 cgccaacatt tccggcctcg gcctggacaa ggtcgggatc gaactgaacg ccaagaacgc
      241 gatcaaagtg gatgagaacc tgcgtaccac gcagccgcac atcgatgcca tgggcgacgt
      301 gacggaccgg gtgaacctga ccccggtggc caccgctgaa ggccacgcgc tggccgacac
      361 gctgttcggt gacaatcctc gcacggttgc attgtggaac ataccctctg cggtattcac
      421 cacgccgccg gttggaattg tcggcctgtc ggaggagcag gcctgcacat tggccccggt
      481 cgatatctac ctgacgcgct tcaccccgat gcggcacaac ctgtccggac gcggccgcaa
      541 gacgacgatg aagctggtgg tggacaaagc gacgcagaaa atcgtcggtg cccatatgct
      601 tggcgaagac gcgccggaga tcatccaggg catcgccatc gccgtcgtga tgggcgccac
      661 gaaggccgac ttcgaccgga cgatcggcat ccatccgacg gcggcggagg aattcgtcac
      721 actacgcacc ctcacaagga caacggctct ggccgaggca gcggaataac cctcacggcc
      781 ccgtacggcc gtcgggtaaa cctgaacacg aaacaattcg gcagaagacc gattgcggag
      841 gatacgatga agcgacgttc attccttgct gccgccgcgg cgaccgcact cgctcggcct
      901 gccgtcgcta atggcacgaa gccgctgatc tttgtgccgc aggcgaacct gaccagcctt
      961 gatcccgtct ggaccacggc gaccgtaacg cggaacttcg cgctcatggt ttatgacatg
     1021 ctttacggcc gcgacgagaa tttcgtcccc cgcccgcaga tggtcgaagg tcatgtcatc
     1081 gacgatgacg gcaagcgctg gacgatgaag ctgcgcgacg gactgatgtg gcacgacggc
     1141 accccggtgc tggcacgcga ctgcatcgcc agcctgcagc ggtggatgaa gcgcgacgcc
     1201 gccggcgtca ccatcaacct gcgggtcgat gcgatcgagg cgccggacga tcgcacgctt
     1261 gtttggcgcc tgcgcaaacc attcccgctg ctgccatttt ttctttcgaa agttcagcct
     1321 cagccggtga tgatgccgga gcgcattgcc gccaccgtcg atccattcaa accggtaacc
     1381 gagatcgtcg gcagcggccc gttccggttt ctggcgaaag aatacgtttc cggcgccttt
     1441 gcggcgttcg agaaattcga ccgctacgtt cctcggaacg aaccggcgtc ctatacggcg
     1501 ggtggccatc atgtgctggt ggatcgggtg gaatggcggg tcatccccga cagcgccacc
     1561 gccgccaacg cattgaccgc cggcgaagtg gattggctgg agctgccgca acccgatctg
     1621 ctgccgatgc tgaaacagaa ggaaggcgtc accaccgggc tgctcgatct ttacggcacg
     1681 gttggcatcc tgcggccgaa ccacctgaac gcgccgacca gcaatgtcgg tgtccggcgc
     1741 gcgatgttcg ccgcgatcga ccagcgggag atcatgctgg cgacgatggg cgaggatccg
     1801 tccacctggc gcgcgcccat gggatacttc ctgccgaact ccaaatccgc caacgacgcc
     1861 gggatggact tcgtccgcaa gcggcacagc accgatgagg tcaagcggat gctggatgag
     1921 gcgaaatacg gcggcgagaa gatcgtcttc ctgcatccca cggaccagct cgcctacaac
     1981 gctttcagca cggttgtggt cgatgcgttt cacaaagtcg ggctgaatgt cgatgagcag
     2041 atgaccgact ggggcaccgt ggtgcagcgc cgcccatcga aggaaccgtt ggacaagggc
     2101 ggctggtcga tgttccccgc tggcgcgcca ggtcccgaat tcgtcgatcc gctgctggcc
     2161 aacaccctgc gcagcaatgg gtccaaggcc tggtttggct ggcccgacga tccggtgctg
     2221 gaggcggcct acgaagcatg gatcgacgcg ccggacgagg ccgaacggca caagcaggcg
     2281 acggcgttcc aggcggcggc gttccagtcg gttcccagca ttccgctggg acagtacctg
     2341 ccgcatgcgg cctggcggtc aaatatcaca gggttggtaa aggggtccgc gccggtgttc
     2401 tggggcgcca agaaggaatg acctcgcgtg gcagagggtg acctgccggg ggagccggac
     2461 gcgaacaccc tcatccgcgg gttcgaggcg acggatgcaa ccgcggtgcg ggacctgttc
     2521 gtccttgtaa accggctgct gtcgccgccg cacctgcgca ctgcgttcga agcctatatc
     2581 gcccggtcgt tgtccgagga gatcgggcgc atcccggcct attacgcgga gcacgccggt
     2641 gggttctggg tcgcctgccg gggtgaggac gtcgtcggga tgttcggcct tgaggccgcc
     2701 ggtccgaacg ct
//
LOCUS       DASWQN010000824         3023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_739141, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000824 DASWQN010000000
VERSION     DASWQN010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739141"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..535)
                     /locus_tag="VGC82_13935"
     CDS             complement(257..535)
                     /locus_tag="VGC82_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424418.1"
                     /translation="MASIDPVIERIEAYPWPRGGVSVRKLHGGYSLSSARTGAPVARL
                     KPIPQDDRYEVLWWRRDAWGPAGPFGAGLSLDDALTFIASEPPFWIQA"
     gene            646..1959
                     /locus_tag="VGC82_13940"
     CDS             646..1959
                     /locus_tag="VGC82_13940"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0424419.1"
                     /translation="MAPFLAAFCHRSRDTAAALAVGALLAIGPRTVTNCLRALGLAEH
                     PSFTAFHRVLNRNVWSGLALARTLLRLLVPMFEPGRRFVIIGVDHTLERRRGRRIEPA
                     SHFHDAVRSTAKQKVTSRGLRWISAMLLVNVPFAGRIWGLPVLTALTPSQAWCEHHKR
                     RYRPVTKWAELLLLTLHRWLPDRVIVAVMDGEFAAIDLLHRLRRYVVAITRLRLDACL
                     FDPPLEYKGRGRYPKTGARQPPLKARITDPATVWKRAVQASRTSWRSGGWIDYASGTA
                     LWYHAGKAPLPIRWVLVRYPDKRHDTEAFLCTDTQMAPLDVLDCYNRRWSVETTYEEA
                     RAHLGVETQRQWSDPAIFRTTPLLFGLYSLVTLYVQQNAERLALSPRRAAWYPKPAAT
                     FADALARLRQHVWFEHFVTSADAPNMKECLPPDLQRLIQLACYAP"
     gene            2122..3023
                     /locus_tag="VGC82_13945"
                     /pseudo
     CDS             2122..3023
                     /locus_tag="VGC82_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881221.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          538 a         1004 c          922 g          559 t
ORIGIN      
        1 ccctgacctc cgctgcgctc cacggctcag agatagtgtc gggacgaaag gcgtcccgcc
       61 atttcagccc gtcgccttcg gcgcgggccg aacaaaaggc tgttttgtca gatgactaaa
      121 atatcgccga aaacgccggc gttgccgggt tgccgtccag catttgcagg tggcaagaca
      181 ccgttgttgc ccggccgccc aggaccatag gaacttaagg agcctcctgg actttgccca
      241 tcgcgggtct gtgaggtcag gcttgaatcc agaagggcgg ctccgaggcg atgaaggtga
      301 gagcgtcgtc gagtgataat ccggcgccga atggcccggc cgggccccag gcatcgcggc
      361 gccaccacag cacctcatag cggtcatcct gcggtattgg tttgagcctg gcgacaggtg
      421 ccccagtacg agccgatgac aagctgtagc cgccatggag cttgcgaacg ctgacgccgc
      481 cgcggggcca gggataggcc tcgatccgct cgatcacagg atcaatcgac gccattacga
      541 tcttctccga ccccgttccg tttgaccggc agacaccgta ccatcgcagg aggttctgtc
      601 atggtccgcc ccgatcggcc tctcccacaa gctttcgttg cttggatggc acctttcctc
      661 gccgccttct gtcaccgcag tcgcgatacc gccgcagcct tggcagtcgg cgccttgctg
      721 gcgatcgggc cacgcacggt taccaactgc ctgcgtgctc tcggcctggc cgagcatcca
      781 agcttcaccg cctttcaccg cgtgctcaac cgcaatgtct ggtcgggctt ggcgctggct
      841 cggaccttgc tgcggctgct ggtcccgatg tttgaaccgg gccggcgctt cgtcatcatc
      901 ggcgtcgatc ataccctgga acgccgccgc ggtcgccgca tcgaacccgc cagccacttc
      961 cacgatgccg tccgctccac cgctaagcag aaagtcacca gtcgagggtt gcgttggatc
     1021 agcgccatgc tgttggtcaa cgtgccgttc gccggtcgca tttggggttt gccggtgctg
     1081 accgcgctga cgccgtccca ggcttggtgc gagcatcaca agcggcgcta tcggccggtg
     1141 accaaatggg ccgaacttct gctgctgaca ctgcaccgct ggctaccgga ccgcgtgatc
     1201 gtggcggtca tggacggtga attcgccgcc atcgacctgc tgcaccggct gcgccgatac
     1261 gtggtcgcca tcacccggct gcggctggat gcatgcctgt tcgatccccc tttggagtac
     1321 aagggacgcg gccgctatcc caagacaggc gcgcggcaac caccgctgaa ggcgcgcatt
     1381 accgatcctg ccacggtctg gaagcgggcg gtacaggcca gccgcacgtc ctggcgtagc
     1441 ggcggctgga tcgactatgc ctccggaacg gcgctgtggt accacgccgg caaggcgccg
     1501 ctgccgatcc gttgggtgct ggttcgttat ccggataaac gtcatgacac cgaggccttt
     1561 ctgtgcaccg acactcaaat ggccccactt gacgtgctcg actgctacaa tcgccgctgg
     1621 tccgtggaaa cgacttacga agaagcgcgc gcccatctcg gtgtcgagac ccagcgccag
     1681 tggtcggatc cggcaatctt tcgcaccacg ccgctgttgt tcggtctgta ctcgttggtc
     1741 accctctacg ttcagcagaa cgccgagcgg ctggcactct cgccgcgccg ggccgcctgg
     1801 tatcccaaac cggcggccac cttcgccgac gctctggcgc gtctgcgcca gcatgtctgg
     1861 ttcgagcatt tcgtcacgtc ggccgatgcc cccaacatga aggaatgcct cccgcccgat
     1921 ttgcaacgat tgatccagct tgcgtgctac gcaccctgaa ctgacatatc agtcgacagc
     1981 cgcgacaaaa tgggcttccc accggggagt ccgggtggga aagccgaact cacagccgga
     2041 gttctgacct gtttccgata tcacagaccc gcgatgggca aagtccagga agcctattca
     2101 cttccgtaga accgctctgt cgtgccgttc aaacaccatg ctgaacaccg ctatcacatc
     2161 cccaagcccc ggtcgggtca cgaactggcg ggagtacgac acggcattga agcgccgggg
     2221 tagtctgacc gtttggttta ccgacggggc gatccaggcg tggcaagcgg aacggcggac
     2281 cacgccaggc ggtcagtcgc actactcgtc cctagcgatc accacggcgc tgacggcctg
     2341 gcgctgcggc agaccgaggg ctgatcggct ccgtcatcgg gctgctcggg cttgacctcg
     2401 ccgtgccgga tcattccaca ctgagccagc gggccaagac gctgcagctg ccgccgctcc
     2461 ggcagagcgg ttccggctcg ctgcatttac tggtggacag caccggactg agctcggcgg
     2521 agccggcgga tggctggtgg aaaagcacgg cacgtcgcgc cgccggtcct ggcgcaagct
     2581 acacatcggc gttgatgccg tcagcggtga gattctcgcc gtcgaagtga ccaagaaaga
     2641 aatcgacgat gtcacgatga ttggcgcact tctcggccag attgctgatc cccttgcttc
     2701 ctttaccgcc gacggcgatt acgatcagga ccgggtttac caggcggtca ctgatcatca
     2761 tcatcccgat gctgccgtga tcgtgccgcc gcgggcaacc gcggtgccga gtgcctcggc
     2821 cgagaccacc ccaacacaac gcgaccgcca tctgcggata attggggaac gcggtcgcat
     2881 ggcctggcaa aaagccagcg gctacaatct gcgggccaag gtcgaggcgt cgattggccg
     2941 gtataaacgg gtgatcggcg atgcgcttcg atcgcgcgcc gacgagaccg aggttgccat
     3001 cgctgccgct gcgctgaacc aaa
//
LOCUS       DASWQN010000825         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1664034, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000825 DASWQN010000000
VERSION     DASWQN010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664034"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1293)
                     /locus_tag="VGC82_13950"
     CDS             complement(133..1293)
                     /locus_tag="VGC82_13950"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0424420.1"
                     /translation="MDMMDLTFVPASRIVEVARLAFVTPDVDFLCFGESDQATSPAIR
                     DAAVTALMNGETRYPDVRGLPSLRVALSGYLSSLHAKPVAESRIQVTASGMTAITVAL
                     AAVVRAGHRVVVHSPAWPNITNAARLRGAVVENLDLDVQPDGGFRLDLDRLDSMMDGA
                     RAFVLNSPGNPTGWTASEAELLAIRDIARRHGAWLISDEVYSRLVYDGSAAAPSMLDI
                     AEADDRIIVCNSFSKTWIMTGWRLGWLVVPEGTAEQFVDIVETVHSGVAPFIQQGGLA
                     ALGETNAIERFRDHCAAGRELAGQALAGLNGIRYQPPAGAFYAFMAVEGLHDSLDLAK
                     KLVTRHGVAVAPGVAFGSAGEGWLRVCFAQSAPMMERAMQRLRDGLRAEMGS"
     gene            complement(1471..2715)
                     /locus_tag="VGC82_13955"
     CDS             complement(1471..2715)
                     /locus_tag="VGC82_13955"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424421.1"
                     /translation="MASLSAIGGVLAQRNARIFYSGSIVCWTGSWVQRIATDWLAWEL
                     THSALWVAIIAFCNLAPSVIVSPIAGAVADRVDRVRLTMASQFVTVAQAIILVALILS
                     GLIRVEFMAFLTLCNGAAETFAQPARQCLIPGLVPRSHLPGAVALNSLTYNAARFVGP
                     AISGPMIAVWGVVPSIVVNALAFLYASLTMHMLRLDPSIRRGERSGFSVLHDAFDGIR
                     YVARHRGIGPLMLYAATVGMTLRAVPEMLPPYVADQFGRGAEGLATLASTMGCAALIG
                     GLAVAIRGRMTGLTRIAVGCGLILALATAGFVATHIFAVGVICIAAIGAATTMHGISC
                     QTLLQNSTAPGMIGRVLSTWGMITRAAPAMGALAYGIASEFLGLQIPVLIGAGICVVV
                     WLRTWSRLSHMVPILEGSVTAD"
BASE COUNT          544 a         1016 c          890 g          411 t
ORIGIN      
        1 cctcgatcca gtcgcgcaag gtggaccggg ccggctcacc cgggcgcata tccaggtcgg
       61 gcgcgctttc cggctgaggc gcacggaacg ccggctggga cgggctgccg caaaccccgc
      121 acattggctg gctcatgatc ccatctccgc ccgtagccca tcgcgcagcc gctgcatcgc
      181 ccgctccatc atcggggcag actgggcaaa acagacccgc agccagcctt ccccggcgga
      241 gccgaatgcc acgcccggtg cgaccgcaac gccgtgccgc gtgaccagtt tcttcgccag
      301 atccaggctg tcatgcagcc cctcaaccgc catgaacgca tagaatgcgc cagccggcgg
      361 ctggtagcgg atcccgttca accccgccag cgcctgcccc gcgagttcgc ggccggcggc
      421 acaatggtcg cggaaccgct cgatggcatt ggtttcgccc agtgccgcca ggccgccctg
      481 ctggatgaac ggcgcaacgc cggaatgcac ggtttcgacg atatcgacga actgctccgc
      541 cgttccctcg ggcacgacca gccagcccag ccgccagccg gtcatgatcc aggtcttgga
      601 aaagctgttg caaacaatga tccggtcatc cgcctccgca atgtccagca tcgacggcgc
      661 cgcggccgaa ccgtcgtaaa ccagccgcga gtaaacctcg tccgagatca gccacgctcc
      721 gtgccggcgg gcgatgtcgc ggatcgccag caattccgcc tcggatgccg tccaccccgt
      781 cgggttgccc ggggagttca ggacgaaagc ccgcgccccg tccatcatgc tgtccagccg
      841 gtcgaggtcg agcctgaagc cgccatccgg ctgtacgtcc agatcgaggt tctcgaccac
      901 ggctccgcgc agccgcgcgg cgttggtgat gttgggccag gccggactat gcacgacgac
      961 gcggtggccg gcccgcacca ccgccgccag cgccacggtg atcgccgtca tgcccgacgc
     1021 cgtgacctgg atgcggcttt ccgccaccgg tttggcatgc agcgaactca agtagcccga
     1081 cagcgcgacg cgcaatgacg gcaggccacg cacgtccgga tagcgcgtct ctccgttcat
     1141 cagtgcggtc accgccgcat cgcggatggc cggcgaggtc gcctggtcgg actcgccgaa
     1201 acacagaaaa tccacatccg gcgtgacgaa agccagacgg gcgacctcga cgatgcgcga
     1261 ggccggaaca aacgtaagat ccatcatgtc catataggcg ttgcccctcc gagatgggat
     1321 caccccgggc gcaatttacc cttccggtgt cgccgaatat aaggaaatcg caaccgcctg
     1381 acggtcacgc gcctgattcc gcggttcctc actcatcacg gcgaagaacc ggaccgtatc
     1441 gcggatcacc gagcagccgc ggcaaatcca tcagtcggcc gtgacgctgc cttccaggat
     1501 cggaaccatg tgggaaagac ggctccaggt tctcagccag accacaacac aaattccagc
     1561 gccgatcagc actgggatct gcagtcccag gaactccgat gcgatgccgt aggccagcgc
     1621 ccccatcgcg ggcgccgccc gggtgatcat gccccaggtg ctcagcaccc ggccaatcat
     1681 ccccggcgcg gtggaattct gcagcagggt ctggcaggaa atgccgtgca tcgtcgtggc
     1741 ggcaccgatc gcggcgatgc agatcacgcc aacggcaaat atgtgggtgg caacgaagcc
     1801 ggcggtcgcc agcgctagaa tcaggccgca gccgaccgcg atccgggtca ggccggtcat
     1861 gcgtccgcgt atcgcgaccg cgagtccgcc gatcaacgca gcgcaaccca tggtgctggc
     1921 cagggttgcc aaaccctcgg caccgcggcc gaactggtcg gcgacataag gcggcagcat
     1981 ctccggcacc gcccgaagcg tcatgccgac cgtcgcagca tacagcatca acggtccgat
     2041 gccgcgatgc cgggcgacat accggatgcc gtcgaacgca tcatgcagca cgctgaaccc
     2101 gcttcgttcg ccgcgccgga tcgatgggtc cagccgcagc atgtgcatcg tcaggctggc
     2161 atacaggaag gccagggcgt tcaccacgat cgacggcacc acgccccaga ccgcgatcat
     2221 cggccctgag atcgccggcc ccacgaagcg cgcagcgttg taggtcaacg agttcagtgc
     2281 caccgcaccg ggcagatgcg accgcggcac cagcccgggg atcaggcact gccgggcggg
     2341 ctgtgcgaac gtctccgccg cgccattgca cagcgtcaga aaggccatga actcgacccg
     2401 gatcaggccg ctcaggatca gcgcgaccag gatgatggcc tgtgcgaccg taacgaactg
     2461 gcttgccatg gtcagccgca cgcggtccac gcggtctgcc accgccccgg cgatcggcga
     2521 aacgatgacc gaaggtgcca ggttgcagaa cgcgataatg gcgacccaaa gtgccgagtg
     2581 ggtcagctcc cacgccagcc agtcggtggc gatgcgctgc acccatgacc ccgtccagca
     2641 aacgatgctg ccggaataga agatccgggc gttgcgctgt gcgaggacgc cgccgatcgc
     2701 cgacagactg gccatgccag ctcagcgcgg ggcaggcgga gacggcaggc tgcgttcggt
     2761 ctcggtcccg gtgttccgca cgcagtcgct gatcctccgg tcgtgcgcga acagctccga
     2821 caagccccga tcggggccaa ccggaccgta attcgccgaa t
//
LOCUS       DASWQN010000826         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_80879, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000826 DASWQN010000000
VERSION     DASWQN010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80879"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..597
                     /locus_tag="VGC82_13960"
     CDS             67..597
                     /locus_tag="VGC82_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0424422.1"
                     /translation="MLKARSHFVAPPKLPPPTPVVDVSDAEMRLAQDLLFFAYRDYTN
                     VADKVLDELGLGRAHHRALHFIGRNPGITVSGLLAILRITKQSLARVLNALVDEGFVS
                     QAPGFEDRRLRLLTLTEKGRSLEKRLFDQQREQISRAYQEAGPEAVSGFRTVMRAIMD
                     KGARAYMDGTGSAEDA"
     gene            602..1303
                     /locus_tag="VGC82_13965"
     CDS             602..1303
                     /locus_tag="VGC82_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0424423.1"
                     /translation="MPESNHILVVEDDPRLRERLARYLTGEGFRVTAAGDAAEARIQL
                     RAIDPDLMVLDVMMPGESGLDLTQSLRQERNPDMPILLLTARSAPEDRIAGFEAGADD
                     YLGKPFEPRELVLRIRALLRRAPPPLAPQMPMGPVRIGEAEYDVERGELRDGSGPIRL
                     TGGESALLTALARKANEVLSREEIAEALGMDDSGERAIDVQVVRLRRKIEADPREPRF
                     LHTVRGRGYILKPGI"
     gene            1304..>2170
                     /locus_tag="VGC82_13970"
     CDS             1304..>2170
                     /locus_tag="VGC82_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY0424424.1"
                     /translation="MRAWIRLPFGARIKRMMPRSLLGRTLLIMLVPLVLVQAIALQLF
                     YGNHLGVVSRRLSGAIAGEIYYTVELLHRFQTPEEREWALQLAREQFVLGIKMEPGAV
                     LEPRQRENVLGPMDDDFAAALTEKFNVPFTMDWTSDPRSVLIRLQLPDGVLDVEAPRK
                     RLAIGTVFLFVGWLVGSALFLFGIAALFMRNQVRAIRRLARSAEAFGMGRDVPQIRPE
                     GAVEVRQAAAAFNRMQERIRRFLAQRTEMLAGVSHDLRTPLTRLRLALAMLPRTEELQ
                     PDVAEMTADVEEM"
BASE COUNT          370 a          696 c          744 g          360 t
ORIGIN      
        1 ctcatttaga acgcatgtgt ttattaagcc aatgatactg acctatatca agatcgaatg
       61 ccaggcatgc tgaaagccag atcacacttt gtcgcgccac ccaagctgcc gccgccgacg
      121 ccggtggtcg atgtgagcga cgccgagatg cgccttgccc aggatctgct gttcttcgcc
      181 taccgcgact acaccaacgt ggcggacaag gtcctcgacg aactcgggct ggggcgggcg
      241 caccatcggg cgctgcattt catcgggcgg aatccgggca tcacggtgtc gggtttgttg
      301 gcgattctgc gcatcaccaa gcaatccctg gcgcgcgtgc tgaacgccct ggtcgatgaa
      361 ggcttcgtca gccaggctcc ggggttcgag gatcgccggc tgcggctgct gaccctgacc
      421 gaaaagggcc gatcgctgga aaagcggctg ttcgaccagc agcgcgaaca gatttcgcgg
      481 gcctaccagg aagccggccc cgaagcggtg tctggctttc gcactgtgat gcgcgccatc
      541 atggacaagg gcgcacgcgc ctatatggac ggcaccggtt cggcggagga cgcctgacat
      601 catgcccgaa agcaaccaca tcctggtcgt ggaggacgat ccccgcctgc gcgaacgcct
      661 ggcgcgctac ctgaccggcg agggtttccg ggttaccgcc gccggcgacg ccgccgaagc
      721 gcggatccag ctacgggcga tcgatccgga cctgatggtt ctggacgtga tgatgccggg
      781 cgaaagcggc ctcgacctga cgcagtcgct ccgccaggag cgcaatcccg atatgccgat
      841 cctgctgctg accgcccgca gcgcaccgga agaccgcatc gccgggttcg aagcgggggc
      901 cgacgactac ctgggaaagc cgttcgaacc gcgtgaactg gtgctgcgga tcagggcgct
      961 gctgcgccgg gcaccgccgc cgctggcacc gcagatgccg atgggaccgg tgcggatcgg
     1021 cgaagcggaa tacgatgtcg agcgcggcga actgcgcgac ggcagcggcc cgatccggct
     1081 gaccggcggc gaatcggcgc tgctgaccgc actggcccgc aaggccaacg aagtcctgtc
     1141 gcgtgaggaa atcgccgagg cgctggggat ggacgattcc ggcgaacgcg ccatcgacgt
     1201 tcaggtcgtc cgcctgcgcc gcaagatcga ggcggacccg cgtgagcccc ggttcctgca
     1261 caccgtgcgt ggccgcggct acatcctgaa gccgggtatc tgaatgcgcg cctggatccg
     1321 gttgccattc ggcgcccgca tcaagcggat gatgccccgg tcgctgctag ggcgcacgtt
     1381 gctgatcatg ctggtgccgc tggtgctggt gcaggcgatc gcgctgcagt tgttctacgg
     1441 caaccacctc ggcgtcgtgt cccgccggct gtccggcgca atcgcggggg aaatctacta
     1501 caccgtcgaa ctgctgcacc gcttccagac gccggaggaa cgcgaatggg cgctgcaatt
     1561 ggcacgcgag cagtttgtcc ttgggatcaa gatggagccg ggcgcggtgc tggaaccacg
     1621 ccagcgggag aatgtcctgg ggccgatgga cgacgacttc gcggcggcgc tgaccgagaa
     1681 attcaacgtg ccgttcacca tggactggac gtccgacccg cgctccgtgc tgatccggct
     1741 gcaattgccg gacggcgtgc tggacgtgga ggcgccgcgc aagcgcctgg cgatcggcac
     1801 cgtgtttctg ttcgttggct ggctggtcgg atccgcgctg ttcctgttcg gcatcgcggc
     1861 gttgttcatg cgcaaccagg tgcgcgccat ccgccgcctg gcgcggtctg cggaagcgtt
     1921 cgggatgggg cgcgatgtac cgcagatccg cccggaaggt gccgtcgaag tgcgtcaggc
     1981 ggcggcggca ttcaaccgca tgcaggaacg catccgccgc ttcctggcac agcggaccga
     2041 aatgctggcc ggcgtatcgc atgatctgcg cacgccgctg acccgtctgc gcctggcgct
     2101 ggcgatgctg ccgcgcaccg aggaactgca gccggacgtg gccgagatga ccgccgacgt
     2161 agaggagatg
//
LOCUS       DASWQN010000827         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2323693, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000827 DASWQN010000000
VERSION     DASWQN010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323693"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="VGC82_13975"
     CDS             <1..1338
                     /locus_tag="VGC82_13975"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0424425.1"
                     /translation="YSKMLSDITKSVIEVNEPEQASEAIARAFHIAESGTPGPVAVIL
                     PEDIFDETTDAELNLPRPRVVAGPRNQDLERLADLLAKAERPLILAGGALLADSLHDK
                     TVYADLKRLSEEWVLPISPTHRRPQLFDAHHPNYGGYMGIRVPPPLIGEMKKADLIVA
                     LGERLTDSVSQSYTFPAAPQPQLPVVHIWPDANEVGRVWRVDLGMPCDPHEVVKGLLA
                     LQTPPDASKRRGWVDGLHKIHLSLLEKTWEPTTDGINFAAIVCEVDKHLADDAVITSD
                     AGNFGSYIHRYIGFKPGQEFLSSVVGAMGSGMPMAVAGCLRRPGKQVVAFMGDGGALM
                     MGNEIATACQYGGNPIVIISDNSMYGTIGMHSYVRYPDRKFMDATRLTNPDFAAWGRS
                     FGAEGITIREESEIAGGIARAFAVKTKPVVVHCLTSALQMSAWRRFQNGAVLP"
     gene            complement(1902..>2336)
                     /locus_tag="VGC82_13980"
     CDS             complement(1902..>2336)
                     /locus_tag="VGC82_13980"
                     /inference="COORDINATES: protein motif:HMM:NF028151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HEY0424426.1"
                     /translation="LNGVTQSGTAATMYRNATSSFNVGGTTAATAGPADTLTLHLSED
                     AYTGDAQFVLAIDGKQIATPQSVTASHSAGAWQTIAFAGSFGAGSHTVGITFTNDAYG
                     GTAATDRNLYDNGIDVNGQHYGTGVTAMMSNGTTTFNIATTH"
BASE COUNT          399 a          708 c          773 g          456 t
ORIGIN      
        1 tattcgaaaa tgctgtccga catcaccaaa agcgtcatcg aggtgaacga acccgaacag
       61 gcctccgagg ccattgctcg ggcgttccac atcgccgaaa gcggcacacc ggggccggtc
      121 gcggtgatcc tgccggaaga cattttcgac gaaacgaccg acgctgaatt gaacctgccg
      181 cgtccgcgcg tcgttgccgg accgcgcaac caggatctgg agcggctggc ggacctgctg
      241 gccaaggcgg aacggccgct gatcctggct ggcggcgcac tgctggcgga cagcctgcac
      301 gacaagacgg tttacgccga cctgaagcgt ctgtctgagg aatgggtgct gccgatcagt
      361 ccgacccacc ggcgtccgca actgttcgac gcgcatcatc cgaactatgg cggctatatg
      421 ggcatccgcg tgccgccgcc gctgatcggc gagatgaaga aggccgacct gatcgtcgcg
      481 ttgggcgagc gcctgaccga cagcgtcagc cagtcctaca cctttccagc cgcaccccag
      541 ccgcagcttc cggtggtgca tatctggccc gatgcgaacg aagtcgggcg cgtctggcgc
      601 gtcgatcttg ggatgccctg cgatccgcat gaggtcgtca aggggttgct ggccttgcag
      661 actccgcccg acgcgtcaaa acgccggggc tgggtcgatg ggctgcacaa gatccatctg
      721 tcgctgctgg aaaagacctg ggagccgacg accgacggca tcaacttcgc cgccatcgtc
      781 tgcgaggttg acaagcacct ggcggacgac gcggtgatca cgtccgacgc cggcaatttc
      841 gggtcgtata tacaccggta tatcggcttc aagccggggc aggagttcct gtcctcggtc
      901 gtgggcgcga tggggtcggg gatgccgatg gcggtggccg gatgcttgcg ccggccgggc
      961 aagcaggtcg tcgcgttcat gggcgacggc ggcgcgctga tgatgggcaa cgagatcgcc
     1021 accgcctgtc agtacggcgg caatccgatc gtcattatct ccgacaattc catgtacggc
     1081 acgatcggga tgcactccta cgtgcgctat ccggaccgca aattcatgga tgcgacccgc
     1141 ctgaccaatc ccgatttcgc cgcctggggg cggtcgttcg gggccgaggg cattacgatc
     1201 cgggaggagt cggagattgc cggagggatc gcgcgggctt tcgcggtgaa gacgaagccg
     1261 gtcgtggtgc actgcctgac ctcggccctg caaatgagcg catggcggcg gttccagaac
     1321 ggtgcggtat tgccctgacc gttgggagtc gttcggtgcg ggtcgcggga gtaaggcccg
     1381 gccgccgtga gctgctatca gccgctcgcc gcgtccgggg tttctcaata tgcgagagaa
     1441 tttagcctga ttatacaggt cgaaaatgct ttaccccgtt gttaatcgcg tggttcacac
     1501 gcatcgcgcg accagcgcgt cctttggtgg ttcacacgca tcgcgcgacc atcgcgtcct
     1561 ttggacgacg gacgttccgc ccagcgtgat caccctctgg tgtcctaatt tgaggtccgc
     1621 catatccggc gaacctcagt ctcgcgccac tctctctttg gttcagtggc ctgctgatcc
     1681 cttgcgtccg ccgcatgatg cgacgaattt cgggggcagg acactagccg atggttataa
     1741 tcgagcagcc acctgaacga gagcggtttt acgggcactg gaaacagagt cctcttgtgg
     1801 ttgcagagaa agccgtgggt ggcttgcgcc gagcaccgcc atgacacatc gacgccaggc
     1861 ggcacacagg ctcgcaactc ttccagccgg cgtgcgagcg cctagtgagt ggttgcgata
     1921 ttgaatgtgg tggtgccgtt gctcatcatc gcggttacgc cggtgccgta atgctggccg
     1981 ttgacgtcga tgccgttgtc gtaaaggttg cggtcggtgg ccgctgtacc gccataggcg
     2041 tcattggtga atgtgatgcc gacggtgtgc gatccggcgc caaagctccc ggcaaaggcg
     2101 atcgtctgcc aggcgccggc gctgtgcgag gcggtgacgc tctgcggcgt tgcgatctgt
     2161 ttgccgtcga tggccaggac gaactgggca tcgccggtgt atgcgtcctc ggacagatgc
     2221 agggtcagcg tatccgccgg cccggccgtg gccgcggtcg ttccgcccac gttgaagctg
     2281 ctggttgcgt tgcggtacat cgtcgccgct gtgccgcttt gggtaacccc gttcag
//
LOCUS       DASWQN010000828         6265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1135678, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000828 DASWQN010000000
VERSION     DASWQN010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135678"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VGC82_13985"
     CDS             complement(<1..979)
                     /locus_tag="VGC82_13985"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY0424427.1"
                     /translation="MAVSFVGWGISGDLFRLMGPPSWVAKIGGQTIEVPAFQTEFQRA
                     MAQEARDLPQGQEASADVRRRVGQQTLERLIAQTALSQELRDLHVVAPDDAVASVARS
                     MPVFQGSDGKFSKAVFDSVLRNNGFTETRFLAGLRADLAQRQLLSTLSGSVGAPDIEV
                     KPLYEAEFEKRAADMALFPLAAAPEPAAPDASALQRWYDNHPDLYATPEFRRIKAIEL
                     SPQSLSSELTVTDDELRAAYDEHRSEYVSPEKRSAEVISAPDEAKARALAEKWRSGAN
                     WAAMQAAAQAEGASAITEDDATIVQFPDPDLAKAVFSAAADMVSEPVKGQ"
     gene            1646..2863
                     /locus_tag="VGC82_13990"
     CDS             1646..2863
                     /locus_tag="VGC82_13990"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0424428.1"
                     /translation="MPESPADKAMFADSKTRMPLDGITVLDLTLARAGPTAVRHFADW
                     GANVIRIEPPATEGEDVAGRRHGFDFQNLHRNKRAVTLNLKSKEGHEAFMRLAAKADV
                     VLENMRANVKHRLKVSYEDVKAINPRIVYGSISGFGQDGPYGPRAGVDQIAQGMGGLM
                     SITGEPGRGPMRVGIPIDDLTAGNLLALGCMMALFDRERTGVGRWVTTSLLEAQIFML
                     DFQASRWLMEGEVAGQAGNDHPTGIPTGVFPTSDGHINIAASSSRVFTRFCEAIDRKD
                     WLERDDWKTQVGRSKDRRAINAAISEITATKPSNHWIDLFEANGIPCGPIYSIDQVFA
                     DPQVKHLGMATKMSSPHVGEADVVASAINISGFSKAVRLHTPDAGEHGDDILKSVGYT
                     DAELDDMRRKGVI"
     gene            2864..3682
                     /locus_tag="VGC82_13995"
     CDS             2864..3682
                     /locus_tag="VGC82_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY0424429.1"
                     /translation="MLPDHAETRAFAGGKMLAAKDDGVGLITFNQPEKRNAISMEMWT
                     GLGEILDEFSEDSSVSVVIVTGSGNKAFVSGADISQFEKNRNSADAQAEYDRANSIGR
                     NKLIGFKKPVIARIRGFCMGGGLAIAMATDLRIASSDSQFGIPAARLSIAYAPDAVRR
                     LIDLVGPAHARMILYTAKRIDAAEAERIGLINRMTSEEGLNDVVLDIARTIADNAPLS
                     VAASKLTINEMIKDESQRDMDAIRKMTEICFNSADYKEGRTAFMEKRPPRFIGR"
     gene            complement(3744..4103)
                     /locus_tag="VGC82_14000"
     CDS             complement(3744..4103)
                     /locus_tag="VGC82_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424430.1"
                     /translation="MAYTDRRSDGTQYIRLSKRAADEMLSATPAAALSDYLEQCDNRL
                     IPSDTNLFLSAQDRLTRDDIVTTLPLCINIIRIGTTLVLPDGERFGRDDINASLSLRL
                     VLALATLDEISRQQDGG"
     gene            4488..5642
                     /locus_tag="VGC82_14005"
     CDS             4488..5642
                     /locus_tag="VGC82_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0424431.1"
                     /translation="MFELPEEHRMLQDLVAKFVDRDLIPLEKGVLAREMSGQTSGLEP
                     HEEQRLLAKCKELGLWGLDVPEEYGGANLPYAALAGVYEEQGRTCVPFTFPPDSPNLH
                     MLIAVATGDQRTKYLEPYARGEAHSAIAISEPGAGGDPAGMTTRAVRNGSDWVINGRK
                     IWVSRVPQADFVIVMARTGEGKRAEGVTAFIVERGTKGFIIEREIPMLGGQRTYELVF
                     EDCRVPASQLLGVEGKGYAPMQLRLTVRRLQIGAWCIGMSRRALDMMVEHTKQRVTFG
                     QRLADRQAIQWWIADAAIKIHACRLMVYDAATKADAGRDVRTEASMVKLYATEMATEV
                     VDHAMQAFGAMGVAKELPLQLMAQKVRTMRVYEGPSEVHRMAIARRIIGR"
     gene            5639..5974
                     /locus_tag="VGC82_14010"
     CDS             5639..5974
                     /locus_tag="VGC82_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HEY0424432.1"
                     /translation="MSESRVKAGIWVSMALRMGNANGRYGVVLRKGDPDAGGILVVLR
                     KDKEVSVLSQLRSGEGELAWMRATGPDPVDEATADAYVARQVKFDPDLWVVEFEAPDL
                     LPPFDAKLV"
BASE COUNT         1203 a         1945 c         2013 g         1104 t
ORIGIN      
        1 gttgcccctt caccggctcc gaaaccatat cagccgcggc cgagaaaact gccttcgcca
       61 gatcgggatc aggaaactgc acgatcgtcg catcgtcctc ggtgatggcg gatgcgccct
      121 cggcctgtgc cgccgcctgc atcgccgccc agttggcacc cgaccgccac ttttccgcta
      181 gggcacgagc cttcgcttca tcgggcgccg aaatcacctc ggccgatcgc ttctccggcg
      241 acacatattc ggacctgtgc tcatcatagg cggcgcgcag ttcgtcatcg gtgacggtta
      301 gctccgacga aagcgactgc ggcgacaact cgatcgcctt gatccggcgg aactccggcg
      361 tggcataaag atcggggtga ttgtcgtacc agcgctgcaa ggcagaggca tccggagcgg
      421 ccggctccgg cgccgccgcc agcggaaaca gcgccatgtc ggcggcacgt ttttcgaact
      481 cggcttcgta gagtggcttc acctcgatgt ccggtgcgcc caccgatcca ctgagggtgg
      541 acagtaattg ccgttgtgcc aggtcggcgc gcaggcccgc caggaagcgg gtctcggtaa
      601 aaccgttgtt gcgcaggaca ctgtcgaaga ccgccttgct gaacttgccg tcgctgccct
      661 ggaacaccgg catcgatcgc gctaccgatg ccaccgcatc gtccggcgca acgacgtgaa
      721 ggtcgcgcaa ttcctggctg agtgcggttt gggcgatcaa ccgctccagt gtctgttgcc
      781 caacccggcg gcgcacgtcg gccgaggcct cctggccttg cggcagatca cgggcttcct
      841 gcgccatggc ccgctggaat tccgtctgga aagccggcac ctcgatcgtc tggcccccga
      901 tcttggcgac ccaactcggt ggccccatca agcggaaaag atcgccgctg atcccccagc
      961 cgacgaacga aaccgccatc aggacgaaga atcctcgagc gatccaggaa tcagtgtaac
     1021 ggcggaattg agtgatcatg aaacgcggcg ggcccgaata ttagaacggg gcggagcata
     1081 tacggcggcc gttgcgcgat ggccagcttt cggatgggcg ggcggcatag attgccgttg
     1141 gccggttggc ggagcgatgg cagtggctcc gaaccgcggg cgcggatacg gggtcagtgg
     1201 atgatatacc cgccgtccac caccaggtcg tggccgtgga tgttgctgcc ctatgtcgtg
     1261 gcaggccggc gatgaggaag cttggggcgc tgccccacac cctgccaagg gccggcggcc
     1321 cttggaacca cttgttaggg cgtgatcgcc tgaggttgac ccagtcagaa ggcctgagtc
     1381 acgcggcgtc ccaaccggcc gcttcgatct ggctggcttc gctggaatca tagcgatcca
     1441 aaatgcttcg aacctgatca ttcgaatagc ctgaagggct tccgtccggc ccatccaggt
     1501 ctccaaagaa cccaatatca ccggacagat cgtgggctac ctaaaccgga cagatcgtgt
     1561 gctaccgaca tcttcgcgtg cggctcttga gcaaacgaca agaccggcgg attatcgccg
     1621 ccagatgtcc tgggaaggaa ccaacatgcc cgagagtccg gccgataagg cgatgttcgc
     1681 cgacagcaag acccgtatgc cgttggacgg catcaccgtg ctggacctga cgctcgctcg
     1741 tgcgggcccg accgcggtgc gccatttcgc cgactggggt gccaacgtca tccggatcga
     1801 gccaccggcc accgagggcg aggacgtcgc cggccgccgg catggattcg acttccagaa
     1861 cctgcaccgc aacaagcgcg cagtgacgct gaacctgaag tcgaaggagg gccacgaggc
     1921 gttcatgcgg ctggcggcca aggccgacgt ggtcctggag aacatgcgcg ccaatgtgaa
     1981 gcaccggttg aaggtgtcct atgaggacgt gaaggcgatc aatccccgca tcgtctatgg
     2041 cagcatctcc ggcttcggac aggacgggcc atacggtccg cgagccggcg tcgatcagat
     2101 cgcgcaaggc atgggcgggt tgatgtcgat caccggcgag ccggggcgcg ggccgatgcg
     2161 cgtcggcatc ccgatcgacg acctcaccgc cggcaacctc ctggcgctcg gctgcatgat
     2221 ggcgctcttc gaccgtgagc gcaccggcgt tggacgctgg gttacaacgt cattgctgga
     2281 agcgcagatt ttcatgctcg acttccaggc cagccgctgg ctgatggagg gagaagtcgc
     2341 cgggcaggcg ggcaacgacc acccgaccgg tatccccacc ggcgtgtttc cgaccagcga
     2401 cggccacatc aatatcgccg ccagttcgtc gcgcgtgttt acccgcttct gcgaggcgat
     2461 cgaccgcaag gactggctgg aacgtgacga ctggaagacc caggtcggcc gcagcaagga
     2521 ccgccgcgcg atcaacgccg ccatctcgga aatcaccgcg acgaaacctt cgaaccattg
     2581 gatcgatctg ttcgaggcca acggtatccc ctgtggccca atctattcga tcgaccaggt
     2641 gttcgccgat ccccaggtca agcaccttgg catggcgacg aaaatgagca gcccgcatgt
     2701 cggggaggcg gatgtggtgg cgtccgccat caacatctcc ggcttttcca aagcggtccg
     2761 cctgcacacg ccggatgccg gggaacatgg cgacgacatt ctgaaaagcg ttggctacac
     2821 ggacgcggaa ctggacgaca tgcgacgcaa gggagtcatt tgaatgctgc cggatcacgc
     2881 tgagacccgt gctttcgcgg gcgggaaaat gctggcggcg aaggatgacg gcgttggcct
     2941 gattaccttc aaccagccgg aaaagcgcaa cgccatctcg atggagatgt ggactggtct
     3001 cggcgagatc cttgatgagt tctccgaaga cagcagcgtc agcgtggtga tcgtgacagg
     3061 gtcgggaaac aaggcgtttg tttcgggggc tgacatcagc cagttcgaga agaaccgcaa
     3121 cagcgcggat gcgcaggcgg agtacgacag ggccaacagc atcggacgca acaagcttat
     3181 cggcttcaag aagccggtta tcgcccgcat ccgcggcttc tgcatgggcg gcggcctggc
     3241 catcgcgatg gcgaccgatc tgcgtatcgc ctccagtgac agccagttcg gcatcccggc
     3301 ggcacgtctg tcgattgctt acgcgccgga tgcggtgagg cggctgatcg atctggtggg
     3361 accggcgcat gcccgcatga tcctttacac cgccaagcgg atcgatgcgg cggaggcgga
     3421 acgcatcggg ctgatcaacc ggatgacgag cgaagagggt ctgaacgacg tggtgctgga
     3481 tatcgcccgc accatcgccg acaacgctcc gctatccgtc gcggcatcca agttgaccat
     3541 caacgagatg atcaaggacg aaagccaacg cgacatggac gcgatcagga agatgaccga
     3601 gatctgcttc aacagcgcgg actacaagga aggccgcacg gcgttcatgg agaaacggcc
     3661 gccgcggttc attggacgct gatccgtagc gcagcgccgc tgctatctgc cggctcgtgc
     3721 tttcgtgcgg cacgggcgat ggatcagcct ccatcctgct gccgagaaat ctcgtccaac
     3781 gtcgccagcg ccagaacgag cctgagcgac aagctggcat tgatgtcgtc tcggccgaac
     3841 ctttcgccgt ccggaaggac cagcgtcgtt ccgatgcgga tgatgttgat gcagagcggg
     3901 agggtggtga cgatgtcgtc gcgcgtcagg cgatcctggg cgctcaggaa cagattcgta
     3961 tccgatggga tcaggcggtt atcgcactgc tcgaggtagt cactcaaggc agcggccgga
     4021 gtcgctgaca gcatttcgtc ggcggcccgc ttggagagcc ggatgtactg ggtgccgtca
     4081 gagcgacgat cggtgtatgc catggtgccg gtccagcgat tgttatgatc atgccacgct
     4141 aacggaccgg ctcgacctag ccaactggcc tcctgatatc cggaatagcc gacttccggg
     4201 gctcccgcac atgctccaat gccaggtctg cgccagacag cgccggacag gtttacccag
     4261 tcgtaagcga acggccgaac agccgctcca atcgactggc attttcctca ctctcaggtc
     4321 gatggtcctc ccgcagcgca attgcatgga agcggcttgg catcacccca ttgcatccgc
     4381 gtgcataaag cgttctcaca ctgccgggct ttgtgccttc gtgacgaaac gtccggatcg
     4441 tgcttccttc tccgccagcc gaccacgagc caacaggagc atcccatatg ttcgaactgc
     4501 ccgaagagca ccggatgctg caggatctgg tggcaaagtt cgtggaccgg gatttgatcc
     4561 cactggagaa aggcgtcctc gcgcgggaga tgtccggcca gacatcaggc ctggagccgc
     4621 acgaggaaca acggcttctg gcgaagtgca aggaactcgg cctctggggt ctcgatgttc
     4681 cggaggaata cggtggcgcc aacctacctt acgctgcgct ggccggcgtc tatgaagaac
     4741 aaggccgcac ctgcgtgccg tttaccttcc cgcccgacag cccaaacctg cacatgctga
     4801 ttgccgtcgc gaccggtgac cagcggacca aatatctcga accctatgct cggggcgaag
     4861 cgcattccgc catcgcgatt tccgaacccg gcgcgggcgg cgaccccgcc ggcatgacga
     4921 cgcgggccgt caggaacggc tccgactggg ttatcaacgg ccgcaagatc tgggtcagcc
     4981 gcgtcccaca agccgatttc gtcattgtca tggctcgcac cggcgagggc aaacgtgccg
     5041 agggcgtcac cgccttcatc gtggagcgcg gcaccaaagg cttcattatc gagcgcgaga
     5101 tccccatgct gggcggtcag cggacctacg aactggtgtt cgaagactgc cgcgtgccgg
     5161 cgtcgcagct gctgggcgtc gagggtaagg gctatgcgcc catgcagctt cgcctgacgg
     5221 tgcgccgcct gcagatcggg gcgtggtgca tcggcatgtc tcgcagagca ctggatatga
     5281 tggtggaaca caccaagcag cgggtgacgt tcgggcagag gctggccgat cgccaagcca
     5341 tccagtggtg gattgccgat gccgcgatta agatccacgc ttgccggttg atggtttacg
     5401 atgccgcgac caaggcggat gccggccgcg atgtgcgcac cgaagcatcc atggtgaagc
     5461 tgtatgccac cgagatggcg accgaggtgg tcgatcacgc catgcaggct ttcggcgcga
     5521 tgggtgtggc caaggagttg ccactgcagt tgatggccca aaaggtccgc accatgcggg
     5581 tttacgaagg cccgtccgaa gtccaccgca tggccatcgc gcgccgcatc atcggtcgat
     5641 gagcgaatct cgcgtcaaag ccgggatctg ggtcagcatg gcattgcgga tgggcaacgc
     5701 caatggccgc tacggtgtgg ttttacgcaa gggcgatccg gatgccggcg ggatattggt
     5761 ggtccttcgc aaggacaaag aggtctcggt cctgtcgcag cttcgatcgg gggaggggga
     5821 gcttgcctgg atgcgtgcaa ccggccctga cccggtcgat gaggcgacgg ccgatgccta
     5881 tgtcgccagg caggtcaaat tcgaccccga tttatgggtc gtcgagttcg aggcacccga
     5941 tctgctgccg ccgttcgatg cgaagcttgt ttgacgtcgg cgctgcccgg ttccagagcg
     6001 ccgaccacgc gcgccagttt cacgggcgag atgtccttgc cgcggatgac gccatcgctg
     6061 cctctattgg gtggtggcac gcgcgctggg atgaaccgca caccgctcgg tggctgtagc
     6121 caatcccccg cccggtcccg cgcaccgctg gcggacgccc tccgggcctt cgcccgccaa
     6181 agcggatcga aggccgcgcc cgcagcgatt tgttcgaagc agaccttatg tgtgcaggag
     6241 tgccatcaag ccggcagccg gatgg
//
LOCUS       DASWQN010000829         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2060632, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000829 DASWQN010000000
VERSION     DASWQN010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060632"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..>1818)
                     /locus_tag="VGC82_14015"
     CDS             complement(25..>1818)
                     /locus_tag="VGC82_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY0424433.1"
                     /translation="QVADLVADAPRRPIEAALPGQRATITDRNGQPMAMSIRTVSLFA
                     DPRQIGDVDDAARKLKQVLPRLSLEETIDRLKRDKKFIYLAREITPREEQAINDLGIP
                     GVDFRPTQHRQYPLARMAAQVLGGVDVDQSGFAGVEKYFDERLRTNTDPLRLSLDLQV
                     QAAVREELLTAMNTFGAIGGCGIVMDVHTGEVLAMVSLPDYDANSVRTAPAENRFNRA
                     IEGTYEPGSTFKLQTASMALDLGVAHIWDQFDASRNISIGRFTISDFEGKHRWLFLPE
                     VLAYSSNLGAAHIAQAVGAERQRAWLKGMGMFGRTGIELPEAARPLVQSAATWKEIVT
                     LTVGFGHGISVTPLHVVRGTAAIANGGTLIRPTILAQEPNEHPDAVRVMQPSTSDVMR
                     RLMRLVVTDGFGKTAEVPGYYPGGKTGTAEKVTAHGGYKKHANVAAFMSVFPMNAPRY
                     AVYMMLDEPHGNKNTYGYATAGWVVAPAAGKVIARIGPMLGMLPDIVDMPAINQALAI
                     PLQPARPAAGSPARGPGGVTPTADAAPKTAVGTNRPTLTVPATVLPPAISRPVRDIRR
                     QTTAAPVDASPADAAPADTAPAVAVASVAAR"
BASE COUNT          304 a          595 c          598 g          321 t
ORIGIN      
        1 atgttgaaaa tgttccgcat gatgtcagcg agcagcaacg gaggcgaccg cgacggcagg
       61 ggcggtatcg gctggggcgg catcggcggg ggaggcatcg acaggagcgg ccgtggtttg
      121 gcgtctgatg tcccgcaccg gacggctgat ggctggtggc agcactgtcg ccggaacggt
      181 cagggtcggc cggttcgttc ccacggcggt ctttggtgcg gcatccgcgg ttggcgtcac
      241 cccgcctggt ccgcgtgccg gagaacccgc cgccggccgc gccggctgca acgggatcgc
      301 cagcgcctgg ttgatcgccg gcatatcgac gatatccggc agcatgccca gcatcggtcc
      361 gatccgggcg atcaccttgc ccgctgcggg cgcgaccacc cagcccgcgg tggcatagcc
      421 gtaggtgttc ttgttaccgt gcggctcgtc cagcatcatg taaaccgcgt aacgcggcgc
      481 gttcatcgga aagacgctca taaaggcggc gacgttggcg tgcttcttgt agccgccatg
      541 cgcggtgacc ttctcggccg tgccagtctt gccgccagga taatagccgg gcacctcggc
      601 cgtcttgccg aacccatcgg tcaccaccaa gcgcatcagc cgccgcatga cgtcggacgt
      661 cgatggctgc atgacccgga cggcatccgg atgctcgttt ggctcctggg ccaggatggt
      721 cgggcggatc aacgtgccgc cgttggcgat agccgcggtg ccgcgcacga catgcagcgg
      781 cgtgaccgaa atgccgtggc cgaagccgac ggtcaaagta acgatttctt tccaggtcgc
      841 cgccgactgg accaacggcc gagccgcctc aggcagttcg atcccggtac ggccaaacat
      901 gcccatgccc ttgagccatg ctcgctgacg ctccgcaccg accgcctgcg cgatgtgcgc
      961 cgcacccagg ttggacgagt aggcgagcac ttcgggcagg aacagccagc ggtgcttgcc
     1021 ttcgaaatcc gagatcgtaa agcggccgat ggagatgttc cgggacgcgt cgaactggtc
     1081 ccagatatgc gccacgccca aatccagcgc catcgaagcc gtctgcagct tgaacgtgct
     1141 gcccggctca tacgtaccct cgatcgctcg gttgaagcga ttttccgcgg gcgcggtgcg
     1201 gaccgaattg gcatcgtaat ccggcaggct gaccatcgcc agcacttcgc cagtatggac
     1261 gtccatgaca ataccgcagc cgccgatcgc accgaacgta ttcatcgcgg tgaggagttc
     1321 ctcgcgcacc gctgcctgaa cctgcaggtc gagcgacagg cgcaacggat cggtgttggt
     1381 gcgcaggcgt tcgtcgaagt atttttccac gccggcgaag ccgctctggt ccacatccac
     1441 gccaccgagg acctgtgccg ccatgcgcgc caatggatac tgccggtgct gggtcgggcg
     1501 gaaatcgacg ccggggatac ccaggtcgtt gatcgcctgc tcttcgcgcg gcgtgatctc
     1561 gcgcgccaga tagatgaact tcttgtcgcg cttcagccgg tcgatggttt cctcgagact
     1621 gagccgtggc aggacctgct tcagcttgcg cgccgcgtca tcgacatcgc caatctgccg
     1681 cggatcggcg aacagcgata cggtgcgtat cgacatcgcc atcggctgac cgttgcggtc
     1741 ggtgatggta gcccgctggc ccgggagcgc cgcctcgatc ggacgccgcg gcgcatcggc
     1801 gacgaggtcc gccacctg
//
LOCUS       DASWQN010000830         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_81389, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000830 DASWQN010000000
VERSION     DASWQN010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81389"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VGC82_14020"
     CDS             <1..522
                     /locus_tag="VGC82_14020"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY0424434.1"
                     /translation="AGKARVAYNKGLPVPDGCLIDAAGEPTNDPTGFIRDHTGALRAF
                     GTHKGSGLAVMCEIMAAAVAGGQGAFHEAKGGVLNSMLATVIDLSRLGDPAKIAADVQ
                     AVKAHVKSARPGPGFDEVLTPGEPEKRYAARRMAEGIDVDDTTWRDIRAAALSLGVTD
                     AEFDRAAGANLGA"
     gene            515..1486
                     /locus_tag="VGC82_14025"
     CDS             515..1486
                     /locus_tag="VGC82_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY0424435.1"
                     /translation="MPELSARESVARTLEIEQRGLEAVRQALDGDLGVAFAAAADVIE
                     AAGGTVIVTGMGKSGHVGRKLAATLASTGTPSYFVHPGEASHGDLGMIRERDVVLALS
                     WSGETPELSDIVAYIRRFGVKLIAITSRPGSALGSAADVGLFLPAAQEACPNGLAPTT
                     STTMQMVAGDALAVLLLERRGFTSVDFQRFHPGGKLGARLSKAASVMHSGDEMPVVPV
                     TAGLSDAIVEMTSKRFGITAVIDAAGDLVGVLTDGDLRRAFKRGFVDGPVVEAMGRRP
                     HSVRPDVLAVEVLAQMNRSRITCIFVVEDRKPVGLIHVHDLLRIGIV"
BASE COUNT          253 a          457 c          641 g          279 t
ORIGIN      
        1 gccggcaagg cgcgggttgc ctacaacaaa gggctgcccg tgccggatgg atgtctgatc
       61 gacgcagccg gtgagccaac aaacgaccca accggattca tccgtgatca caccggggca
      121 ctgcgcgcgt tcggcaccca caagggatcc gggctggcgg tgatgtgcga gatcatggcc
      181 gcggcggtgg ctggcgggca gggcgcgttc catgaggcga agggcggcgt gctgaactcc
      241 atgctggcga cggtaatcga tctgtcgcgg ctgggggatc ctgcgaagat cgcggccgat
      301 gtgcaggcgg tgaaggcgca tgtgaagtcg gcgcggccgg ggccggggtt cgatgaggtg
      361 ctgacgccgg gtgagccgga gaagcggtat gcggcgcggc gaatggcgga gggcatcgac
      421 gtcgatgaca ccacctggcg cgacattcgc gcggcggcgt tgtcgctggg cgtgacagac
      481 gccgagttcg accgggcggc cggggcaaac ctgggtgcct gaactgtcgg cgcgcgagag
      541 cgtcgcccgg acactggaga tcgagcaacg tggcctggaa gccgttcggc aggcgctgga
      601 cggcgacctt ggggtggcgt tcgcggccgc ggcggacgtg atagaggcgg ccggcggcac
      661 cgtcatcgtc accgggatgg gcaagtcggg ccatgtcggg cgaaagctgg cggcgacgct
      721 ggcctcgacc gggacaccca gctacttcgt gcatcccggg gaggcgagcc acggcgatct
      781 ggggatgatc cgggagcgcg acgtggtgct ggcgctgtcg tggtcgggcg agaccccgga
      841 actgtcagac attgtcgcgt atattcgccg gttcggcgtg aagctgatcg cgatcacgtc
      901 gcggcctggc agtgcgctgg gttcggcggc tgatgtcggg ctgtttctgc cggcggcgca
      961 ggaggcttgt ccgaatgggc tggcgccgac cacctcgacc acgatgcaga tggtggccgg
     1021 cgatgcgctg gcggtgctgc tgctggagcg gcggggcttc acctcggtgg atttccagcg
     1081 gtttcatcct ggcgggaagc tgggggcgcg gttgtcgaag gctgcaagcg tgatgcacag
     1141 tggcgatgag atgccggttg tgccggtcac cgcgggcctg tcggacgcca ttgttgagat
     1201 gacatcaaaa cgttttggca tcaccgccgt gatcgacgcc gccggtgacc tggtaggcgt
     1261 gctgaccgac ggcgacctgc ggcgggcctt taaacgcggc tttgtcgatg gaccggtcgt
     1321 tgaggcgatg gggcgccggc cgcacagtgt tcggcctgac gtgctggcgg tcgaggtgct
     1381 ggcgcagatg aaccggtcgc ggatcacctg catctttgtc gtcgaggatc gcaagccggt
     1441 cgggctgatc cacgtgcacg acctgctgcg gatcgggatt gtctagctga ggacgttgtt
     1501 tagctgggga aacggtggga cattctgaga agccagcggg ttccggaagg tgacgcgctg
     1561 catcgccggg catcaacggg gccgatgggt taaaactccg ttgcccgcag ggttcgcgcc
     1621 agccggtcca
//
LOCUS       DASWQN010000831         3701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2323875, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000831 DASWQN010000000
VERSION     DASWQN010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323875"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VGC82_14030"
     CDS             <1..197
                     /locus_tag="VGC82_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424436.1"
                     /translation="LYVYVVKPDNTILAQNVKAGPSVQGFTEITDGLAQGQTVVLSGH
                     SRIAPGRLVKPSSASAGSVP"
     gene            194..3295
                     /locus_tag="VGC82_14035"
     CDS             194..3295
                     /locus_tag="VGC82_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0424437.1"
                     /translation="MSIVRPFIHRRIGTSLLALGVIVVGMVAYLQLPISALPLVDFPT
                     IQIQAKLPGASAQTMATSVATPLERMLSNVSGVTQMTSSSSLGMTNIVLQFDLSRNID
                     GAAQDVQTQINAAGGLLPKDMPDPPTYWKVNPANASIISLALTSPTLPLTELDRYAED
                     FIAQPIAQLPGVGLVDYHGQVRPAVRVRIEPDRLAGLGLTLEDVRAVIGNATVDAPKG
                     TLNGPHQAVVLNATDQLLSADAYRSLVIAYRNGAPIHLQDVGSVDDAPEDVRSAAWFQ
                     GQRAIIVDAAQQPGSNVVATIQAIKAQLPAIAATLPAAAELHIVGDRTQTIHASVRDV
                     QFTMLVSIALVVMVIFVFLRNLWATLIPSLTIPLSLIATFAVMYVLGYSLDNLSLMGL
                     TIAVGFVVDDAIVVIENIMRYIEAGRPRLEAALAGVKEVSFTIISMTVSLIAVFIPVL
                     LMGGLVGRLFREFAVTVSVAIAMSGVVSLTVTPMLCGWLIKRDAHTEPGRIYQWSEKL
                     FQVITAGYGRMLDRVLRHQRATLLVMLATIVLTGWLFIQIPKGFFPQVDSGLILGTAR
                     AAPDISFDAMSQRIKALADIAMRDPGVSTVDYWIGANPTVSQGRLLINLKPLDQRITT
                     ADQILARLRPLMARVEGITLGMQVRQDIQVGGRSSAAQYQYTLQAADVDELYKWSTLL
                     EQKLSDLPQLRDVSSDRQASATSATLDIDRATAARMGVSIRAIDETLYDAFGQRQVAT
                     LYSPLNQYHVILELAPRFQTTTDALDRLYVRSATTNKLVPLNLLVRIQQGVSPITINH
                     QGLFPAVTLSFNVAPGVALGDAVAAVQAVEQQADIPANVTGTFQGAAQAFQNSLQSQP
                     WLILAAIVTIYIVLGVLYESVIHPLTIISTLPSAGVGALLGLWLTGQDLSVIGMIGIL
                     LLIGIVKKNAIMMIDFALVAERVEGKSSFDAIRQACLLRFRPIMMTTFAALLGALPLA
                     LGHGAGAELRRPLGLAIVGGLIVSQALTLFTTPVVYLSFDRLQRSIRIRTNARAGLIV
                     TIK"
BASE COUNT          644 a         1182 c         1152 g          723 t
ORIGIN      
        1 gcctgtacgt ctatgtggtg aagcccgaca acacaatcct tgcccagaac gtcaaggccg
       61 gccccagcgt ccagggattc accgagatca ccgacggact ggcccagggg cagaccgtgg
      121 tgctgagcgg gcattctcgc atcgctccgg gccggctggt gaagccctct tcggcttccg
      181 ccggaagcgt gccatgagca tcgtccgccc gttcatccac cgtcgcatcg gcacctcgct
      241 gctggcgctc ggcgtcatcg ttgtcggcat ggtggcgtat ctgcagctac cgatctcggc
      301 gttgccgctg gtcgacttcc cgaccatcca gatccaggcc aagcttccgg gagccagcgc
      361 gcagaccatg gcaacatcgg tcgccacacc gttggaacgg atgctgtcga acgtctcggg
      421 cgtgacgcag atgacctcat caagctcgtt gggtatgacc aacatcgtcc tgcagttcga
      481 tctcagccgc aacatagacg gggcggcgca ggacgtgcag acgcagatca acgcagcagg
      541 cggcctgctg ccgaaggaca tgcctgatcc gccgacatac tggaaggtca atcccgcgaa
      601 tgcgtcgatc atctcgctgg cgctgacatc accgacgctg cctctgactg aactagaccg
      661 ttacgcagaa gatttcatcg cccagccgat cgcacagctt cctggggtcg ggctggtcga
      721 ctaccacggg caggttcgtc ccgcagtgcg ggtgcgcatc gagccggacc gcttggctgg
      781 cctggggctg acgctggaag acgtgcgcgc ggtgatcggc aatgccacgg tggatgcacc
      841 gaaaggcacg ctgaacggtc cgcatcaggc ggtggtgctg aatgccaccg accagttgct
      901 gagcgccgac gcctatcgct cgcttgttat cgcctaccgc aacggcgccc cgattcacct
      961 gcaggatgtc ggatccgtcg atgatgcgcc ggaagacgtg cgatccgcgg catggttcca
     1021 aggtcagcgc gccatcatcg tcgatgcggc gcagcagccg ggctccaacg tcgtcgccac
     1081 gatccaagcc atcaaggccc agcttccggc gattgcggcc acgcttcccg ccgcggcaga
     1141 actgcatatc gtcggcgatc gcacgcagac tatccacgcc tcggtcaggg acgttcagtt
     1201 caccatgctt gtcagcatcg ccctggtggt catggtgatc ttcgtattcc tgcgcaacct
     1261 ctgggcgacg ctgatcccca gcctgacgat tccactgtca ctgatcgcca ccttcgcggt
     1321 gatgtatgtg ttgggctaca gcctcgacaa tctctcgctg atggggctga ccatcgccgt
     1381 gggcttcgtc gtcgacgacg ctatcgtcgt catcgagaac atcatgcggt atatcgaggc
     1441 gggacggcct cggcttgaag ccgcgctggc aggcgttaaa gaggtcagtt tcaccatcat
     1501 ctcgatgacg gtgagcctga tcgcggtgtt catccccgtc ctgctgatgg gcggacttgt
     1561 gggacgcttg ttccgagagt tcgccgtcac cgtcagcgtg gcgatcgcga tgtcgggtgt
     1621 ggtgtcgctg acagtgacgc cgatgctgtg tggttggctg atcaagcgcg acgcgcatac
     1681 ggagcctggt cggatctatc aatggtcgga aaagctgttt caagtcatca ccgccgggta
     1741 tggccggatg ctggaccggg tgctacgcca tcagcgcgcc acgctgctgg tgatgctcgc
     1801 gaccatcgtg cttacgggct ggctgttcat ccaaattccc aaagggttct ttccgcaggt
     1861 tgacagcggg ctgatcctgg gcacggcgcg ggccgcgcct gacatatcat tcgacgccat
     1921 gtcccaacgg atcaaggcgc tggccgacat cgccatgcgc gatccagggg ttagtacggt
     1981 cgactactgg atcggcgcca atcccaccgt cagccagggt cggctgctga tcaacctcaa
     2041 accgctcgac cagcgcatca cgaccgccga ccaaatcctg gcgcggctgc ggccgcttat
     2101 ggctagggtc gaagggatta cgctaggcat gcaggtccgc caggacatcc aggtcggcgg
     2161 tcgctcatcg gcggcgcaat accagtacac gctacaggcc gccgatgtgg acgagctgta
     2221 caaatggtcg acgttgctag agcaaaagct gagcgacctg ccgcagctgc gtgacgtcag
     2281 ttcagaccgc caagcctcgg caaccagcgc cacgctcgac attgaccggg caactgcggc
     2341 gcggatgggc gtgagcatcc gtgcaatcga cgagacgctg tacgacgctt ttggtcagcg
     2401 ccaggtggcg accttgtact ccccgctgaa ccagtatcac gtgatcctcg aactggcgcc
     2461 gcgattccag acgacgacag acgcgctgga ccgcctgtac gtgcggtccg ccaccacgaa
     2521 caagctggtc ccgctcaatc ttctggttcg catccagcag ggggtctcgc cgatcaccat
     2581 caaccaccag gggctgtttc cggcggtgac cttgtcgttc aatgtcgcac ccggggtcgc
     2641 gctgggcgat gccgtcgccg cggtgcaggc agtggaacag caggcggata taccggccaa
     2701 cgtcaccggt acgttccagg gagcggcgca agcgttccag aattcgctgc agagccagcc
     2761 gtggctgatc ctggccgcca tcgtaacgat ctacatcgtt ctgggcgtgc tctacgagag
     2821 tgtgatccac ccgctcacca tcatctccac cttgccgtcc gccggtgtcg gcgcgctact
     2881 ggggctgtgg ctcaccgggc aggatctctc ggtcatcggc atgatcggca tcctgctgct
     2941 gatcggcatc gtcaagaaga atgccatcat gatgatcgac ttcgcgctgg tggccgagcg
     3001 cgtggagggt aagtcgtcat tcgacgcgat caggcaggcc tgcctgttgc ggttccggcc
     3061 tatcatgatg accacgttcg cggcgttgct cggtgcactg ccgctggccc tggggcatgg
     3121 cgcaggcgcc gaattgcgcc ggccgttggg tctcgccatc gtcggcggcc tgatcgtttc
     3181 tcaggcgctg accctgttca ccacgccggt cgtctacctt tcgttcgatc ggctacagcg
     3241 gtccatacgc atccgcacca atgcccgagc tgggctgatc gtgacgatta agtaatatgt
     3301 tagacatcca atatgaagcg gtcacatggg tttcagcgaa gtgctatggt ttggccgcgc
     3361 agccggcgct cgctgacacg ctgcggtcag cggcggcccg gtaagttatg tgccgatgga
     3421 ggctgcacga tggcctcgtc gcggcgctat ggtgtcaatc gccccgagcg gttttccggc
     3481 cgatagctgt cagcgttgaa gaccatcaag cggtctgcgc cttgtggcgc ggtgcaccac
     3541 tgtgcgaccg caggcgaaac agtggtgcga tggcgccgat gctcttgcct tcctctcggt
     3601 gcagccgaac cgcctcgagc cgctagtgag ggcgcaacgt gtgcttgcgc cgcccccctg
     3661 cttcgcgtcg gccataccgg ccttgacgcg ctcgcgttcg a
//
LOCUS       DASWQN010000832         5010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2192662, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000832 DASWQN010000000
VERSION     DASWQN010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192662"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="VGC82_14040"
     CDS             complement(<1..1301)
                     /locus_tag="VGC82_14040"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0424438.1"
                     /translation="MLRQIIGYVPSTVVPAVVSFVMIYCYTRLLTPSAYGDFSLVFSA
                     VQVVQTVFFYAIPVALTRFYPAALAQQRQDDFLTECYILFYLLSVFVIALIGVVTFSP
                     IAISPVLWVLAILLMIARSAVVLNQAVNRITFKVARFSVIECTHAVLGLGLGLLFIYV
                     LGSSATSVVLGLSVAALICMLLDARPLTLPFRADRTKISRDNIAQLVKFALPLLVVDL
                     TVCILQLSDRFLLDTLGGAEALGIYFVAYNLVERPISLVCTAISTATFPVAVQVLQDH
                     GLSTGGRQAGKNGVILLAVALPACVGLALTAPYIATAMIGANFRAGVAALIPIMCVTA
                     LCRGLSTHFIDHIFHLTGRTVLALRIYGPAAVANIGLNLVLIPRYGMFGAAWAGVACQ
                     AAALVAGWIIGRRAFPIRLPLLDPVKIVTAALLMGLTLFFV"
     gene            complement(1359..2288)
                     /locus_tag="VGC82_14045"
     CDS             complement(1359..2288)
                     /locus_tag="VGC82_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424439.1"
                     /translation="MNNLIAPVFSKWDDKHSALWGNQPLCLEHRLQENPLFSRANLGK
                     LIEKYPTEMYTLVHMGAQGEPKVWRQGVIGDATGEHVIDSIAAGRLWINLLHVNEVDP
                     RYGELLDTIFQEVETRVPGYSTFSRINGILISSPRAQVYYHFDPSGQSLWQIEGAKRV
                     YLYPPVPPFLTPQSLEHVALYRDEVGIKYEPWFDEYASVYELKAGQMMHWPLNSPHRV
                     ENLDRLSISMTTEYFTPEIRRKQMVNTANGLLRKHLGLDPSRNTSGSSFWSKAVLQAA
                     VSRAGLLNRQRAKRREVTFKLDPVRSGEIIDLN"
     gene            complement(2449..3555)
                     /locus_tag="VGC82_14050"
     CDS             complement(2449..3555)
                     /locus_tag="VGC82_14050"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0424440.1"
                     /translation="MRKSWLIPVLVVLAAVVPAALFTDYHLFQMTMVIVYAIAILGLS
                     LLTGFNGQISLGHGAFYAIGAYTTAILMSAWNVPYWLTLPISALVCAGVGFLLGLPAL
                     RLGGLYLALTTYALAIAIPQILKYKAFDEWTGGVQGLVIDKPDAPFGLTLTPDQWLYL
                     FTLGVAVVMYIVAWNIVRGRIGRAMMAIRDHALAAEAMGINIAIVKTRAFALSAMYTG
                     LAGSLGAIAVQFVAPDSFGVFVSIFLFVGLVVGGVSSIGGTLIGAAFIEFVPNLADKL
                     SKAAPSAVYGVILIAVMFLMPAGAGGFLHLMWRKMFPERPGRVAAQPAEPLSVPFETM
                     PALDGPGLGGIAPGAGPVKQALHRNASEPVTRGD"
     gene            complement(3561..4433)
                     /locus_tag="VGC82_14055"
     CDS             complement(3561..4433)
                     /locus_tag="VGC82_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0424441.1"
                     /translation="MLAGVLHQIMSGIATGGIYASVALALVMIYQATHHVNFAQGEMA
                     TFSTYITLTFINLGLPYWVAFAAAVVLSFCIGVAIERLLMRPLAGAPVLASVGVFIGL
                     VLIVNALSGWFFDYTIKQFPTPFPDHSMLGGFLSGHELGATAVTLLVLISVYLFFRHT
                     RLGLAMRAAAFNPTSSRLVGVRVGWMLALGWGLAAAIGAVAGCMVAPIVFLDPNMMGG
                     ILLYAFAGALLGGIDNPLGAVLGGFAVGVIENLGGAYLVGTELKLTLALVIIVSVLTI
                     RPAGLMGRRVYSRV"
     gene            complement(4446..>5010)
                     /locus_tag="VGC82_14060"
     CDS             complement(4446..>5010)
                     /locus_tag="VGC82_14060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0424442.1"
                     /translation="ICGQVRTQGTITLGGQRIDGMATEDIVRLGVAQVPEGRGTFMEL
                     TVEENMRLGAYTRPARDATADFEHMFDYFPRLKERYKQQAGTLSGGEQQMLAIVRALL
                     LRPRLLLLDEPSFGLAPLIVQEIFRILARIKHDEGVSILLVEQNAALALEFSEDAYVL
                     ETGRIVMAGSSADISKDEGIRRSYLGY"
BASE COUNT         1106 a         1575 c         1499 g          830 t
ORIGIN      
        1 ctgacgaaga acagcgtcag tcccatcagc agcgccgctg tcacgatttt gacggggtca
       61 agcagcggca aacggatcgg gaaggctcgc cggccgatga tccatccggc gaccaaagcc
      121 gccgcctggc acgccacgcc agcccaggct gccccgaaca tcccatacct gggtattagc
      181 acgaggttca ggccaatatt ggcgaccgca gctggcccgt aaatccgaag cgccagcacg
      241 gttcgccccg tgaggtggaa aatgtggtcg atgaaatggg tcgaaaggcc ccggcacagg
      301 gcggtcacgc acatgatcgg gatcagggcg gccacgccag cgcgaaaatt cgcgccgatc
      361 atcgccgtgg cgatatatgg cgccgtgagg gccaggccga cgcaggccgg aagagcgacg
      421 gcgagcagaa tgacgccgtt cttgcccgcc tgccggcccc ccgtgctgag cccgtgatcc
      481 tgcaggacct gcaccgcgac cggaaacgtg gccgtcgaga tcgcggtgca gaccaggcta
      541 atgggacgct ccaccaggtt ataggcgacg aaatagatcc ccagtgcttc cgcgccgccc
      601 aacgtatcca gaaggaagcg atccgacagt tgcagtatgc agaccgtaag gtccacaacg
      661 agcagcggca gggcgaactt gacgagttgc gcgatgttgt cgcggcttat ctttgtccga
      721 tcggcgcgaa agggcagcgt cagcggtctg gcgtccagca acatgcaaat cagggcggcc
      781 actgacaggc ccaggacgac cgaggtcgcg gaacttccca atacatagat gaacagaagg
      841 ccaagtccca gcccaagaac ggcatgagtg cattcgataa cgctgaagcg ggcgacttta
      901 aaagtaatgc ggttgaccgc ctggtttaaa accaccgccg aacgagcgat catcaataga
      961 atggccaaga cccacaaaac cggtgatatc gcgatcggcg agaaggtcac aacgccgatc
     1021 aaagcgatga cgaagacgct aagcaagtaa aacaggatat aacattccgt caggaaatca
     1081 tcctgccgtt gctgggcaag ggctgccgga taaaaccgcg tcagtgcaac cgggatggcg
     1141 tagaagaaga cggtctggac gacctgcacc gccgaaaaga ccaagctgaa atcgccgtac
     1201 gcgctcgggg tcagcagccg cgtatagcag tagatcatta cgaacgatac gaccgccggc
     1261 acgaccgtcg atggaacgta gccgattatc tggcgaagca tttcacgcgg ccgacaggct
     1321 gcttgtggtt cagcgaattc agcctgtctt tcccgatttc aattgaggtc gatgatctca
     1381 ccggagcgga ccggatcgag cttgaacgtg acctcccgcc gcttcgcacg ctggcggttc
     1441 agcaatccgg cccggctgac cgcggcttgc aataccgcct tcgaccagaa gctggatccg
     1501 ctcgtgttgc gcgacgggtc gagcccgagg tgcttccgca gcaggccatt ggccgtgttc
     1561 accatctgct tgcggcggat ttcgggcgta aaatactctg tcgtcatgga tatgctgagg
     1621 cgatccaggt tttcgacgcg atgcggggaa ttcagcggcc agtgcatcat ctgccctgcc
     1681 ttaagctcat aaacactggc gtactcgtcg aaccacggtt cgtatttgat acccacttca
     1741 tcccggtaga gggccacatg ctccaggctt tgcggggtga ggaacggcgg cacgggggga
     1801 taaagataga cgcgcttggc accctcgatc tgccacagcg attgccctga agggtcgaaa
     1861 tggtagtaca cctgcgcccg cggggaggag atcaggatgc cgttgatccg gctgaatgtg
     1921 gaatagccgg ggacgcgagt ttcgacctcc tggaatatgg tgtccagaag ctcaccgtat
     1981 cgcggatcga cctcgtttac gtgaagcaga ttgatccaaa gccggcctgc ggcgatcgag
     2041 tcgataacgt gctcgccggt cgcatcgccg atgacgcctt gccgccacac cttcggctca
     2101 ccttgggccc ccatatgcac cagagtgtac atctcggtgg gatacttctc gatcagcttt
     2161 cccaggttcg cgcggctgaa caagggattc tcctgcagac gatgctccag gcacaacggt
     2221 tgattgcccc aaagcgctga atgcttgtca tcccatttgc tgaacacggg cgcgattaag
     2281 ttgttcacac taggtcccct gatggacatc tgacccagta tgtaacacgg cgccttgccg
     2341 gcttcgcgtc acttgtcgga gactaacacc ttcttccgaa acaattactt gttacgctca
     2401 tgttgaccat agcatcgttg accgaacggt cttcgaatgc tgcggacgtc aatcgccgcg
     2461 ggtcacaggt tccgacgcat ttctgtgaag tgcctgctta acgggccctg cgccgggtgc
     2521 gatgccgccc agaccgggac cgtctaacgc gggcatcgtc tcaaatggga ccgacagggg
     2581 ctccgccggt tgcgccgcaa cccgcccggg ccgttcgggg aacatcttcc gccacatcaa
     2641 gtgaagaaag ccccctgccc ccgccggcat cagaaacatc accgcgatca ggatcacgcc
     2701 ataaaccgcg ctaggcgccg ccttggaaag cttgtcggcc aaattgggca cgaactcgat
     2761 gaatgccgcc ccgatcaggg ttccgccgat ggaggacacc ccgcccacaa ccaaccccac
     2821 gaacaggaag atcgagacga acaccccaaa actgtccggt gcgacgaact gcaccgcgat
     2881 ggcaccgagc gatccggcca atccggtgta catggcgctc agcgcgaagg cgcgggtctt
     2941 cacaatggcg atattgatcc ccatcgcttc ggccgccagc gcgtggtccc ggatcgccat
     3001 catcgcccgg ccgatacgtc cgcgcacgat gttccaagcg acgatgtaca tcaccaccgc
     3061 cacgcccagg gtaaacagat acagccactg gtccggtgtc agggtcagcc cgaacggcgc
     3121 atccggcttg tcgatcacca gaccttgcac gccgccggtc cattcgtcga aggctttgta
     3181 tttcaggatt tgcggaatgg cgatcgccag cgcgtaggtg gtcagcgcca gatacaaccc
     3241 gccgagacgc agcgccggaa gacccagcag aaagcccaca ccggcgcaaa ccagcgccga
     3301 aatcggcaag gtcagccagt agggcacatt ccaggcgctc atcaggatcg ccgtggtgta
     3361 cgcgccgatg gcgtagaacg cgccgtgacc taacgagatc tggccattga agccagtcag
     3421 cagcgaaagt cccaggatgg cgatcgcata gacgatgacc atcgtcatct ggaacagatg
     3481 ataatcggtg aacagggcgg ccgggacgac cgccgcgagc accaccagaa ctggtatcag
     3541 ccaggatttc cgcatgtcga tcatacccgg ctatagactc tgcggcccat caggccagcc
     3601 ggccggatgg tcaggacgct gacgatgatc accagcgcca acgtcagttt cagctcggtt
     3661 ccaaccaggt aggcaccgcc gaggttctcg atcacgccga ccgcgaaccc tcccagcacc
     3721 gcccccagcg gattgtcgat cccgcccagc agcgcgccgg cgaaggcata cagcaaaatg
     3781 ccgcccatca tgttggggtc gaggaagacg atcggtgcga ccatgcatcc cgccacggcg
     3841 ccgatcgccg ccgccaggcc ccatcccaaa gccagcatcc acccaacccg gacgccgacc
     3901 agccgggaag acgtcggatt gaacgccgcc gcccgcatcg ccagacccag cctcgtgtgc
     3961 cggaaaaaca gataaacgct gatcaatacg agcagggtga ccgcggtggc gccgagttca
     4021 tggccggaca ggaatccgcc cagcatcgaa tggtcgggaa acggtgttgg aaactgcttg
     4081 atggtatagt cgaagaacca gccgctgagg gcgttgacga tcagcaccag cccgatgaac
     4141 acgccgaccg aggccagtac cggcgcaccc gccaacggcc gcatcagcag acgctcgatc
     4201 gccacgccga tgcagaacga cagcacgacc gcggcggcga acgccaccca gtatggcagt
     4261 cccaggttga tgaacgtcag cgtgatatag gtggagaacg tcgccatctc cccctgcgcg
     4321 aaattcacat ggtgggtggc ctggtagatc atgaccaggg ccagcgccac ggacgcatag
     4381 ataccgcccg tcgcgatacc cgacatgatc tggtgaagaa cgcccgcgag catcgtatcg
     4441 ccccttcagt aaccgagata cgagcggcgg atgccctcgt ccttgctgat atccgccgag
     4501 gaacccgcca tgacgatgcg gccggtttcc agcacgtagg catcctcgga aaattccagc
     4561 gcgagggcgg cgttctgctc gaccagcagg atgctgacgc cttcgtcgtg cttgatgcgc
     4621 gccaggatgc ggaaaatctc ttgcacgatc agcggcgcga ggccgaagga cggctcatcc
     4681 agcaacagca gcctgggacg cagcagcagc gcccggacga tggcgagcat ctgctgctcg
     4741 ccgcccgaca gagtgccggc ttgttgcttg tagcgctcct tcaggcgggg aaagtagtcg
     4801 aacatgtgct cgaagtccgc ggtcgcgtca cgcgccgggc gggtataggc gccgaggcgc
     4861 atgttctcct cgacggtcaa ttccatgaag gtgccgcggc cttcgggcac ctgggcgacg
     4921 ccgaggcgga cgatgtcctc ggtcgccatg ccgtcgatgc gctggccgcc gagggtaatg
     4981 gtgccttggg tacgaacctg gccgcaaatc
//
LOCUS       DASWQN010000833         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2589167, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000833 DASWQN010000000
VERSION     DASWQN010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2589167"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VGC82_14065"
     CDS             <1..227
                     /locus_tag="VGC82_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424443.1"
                     /translation="SFGMRRKRPRSEWLLQGKYRNTAALARAPTGTRYDAVIDIPLKR
                     TTTSVGSPVIQSRQRFVSQGLGQMEAEVRS"
     gene            228..1541
                     /locus_tag="VGC82_14070"
     CDS             228..1541
                     /locus_tag="VGC82_14070"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0424444.1"
                     /translation="MRVAVIGTGIAGNAAAWSLSKRYAVTVYDREARIGGHSHTVAID
                     YDGVPVAVDIGFIVYNELNYPDLTALFAHLGVETIESCMSFAVTADTGRFEWKGGGSN
                     WLETFSGLFAQPRNLLSPSYLRMLRDVLAFNEQSTKDYAAGKLAGLTLGDYFRRNNFA
                     PRLLTDYLAPMGAAIWSAPASEILDFPAENFIAFFSNHRLLQYDRPVWRTVKGGSQRY
                     VEKLTAAFRDQIKPGCAVTAIARTPHGVLVHDSLGNTDIYDHVIVASHSDQALAMLSD
                     ADDHERSILGAIGYSSNKVYLHRDPRLMPKRKRAWASWNFLRWPRKGSVDNDVSVTYW
                     MNPLQGIDADKPLFVSLNPPFEPDPALTFGTYLFEHPQYNAAAFAAQKRLGEIQGKRR
                     TWFCGAWTGYGFHEDGLRSGLAVAEALGAVAPWREPALSLAEAAE"
     gene            1544..>2142
                     /locus_tag="VGC82_14075"
     CDS             1544..>2142
                     /locus_tag="VGC82_14075"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HEY0424445.1"
                     /translation="MPKQITSTERHDAATDAAAALYVGDVMHARLKPIGHRFSYRVMS
                     LLIDLDRLEEADRQSRLFGVNRAALYSFSEADHGPRDGSPLRAYVQHCAAENSIDLTG
                     GRVLLLCYPRLLGYTFNPLSVYFGYRANGELALMIYEVRNTFGDIHPYVVPVQPDQLS
                     DAGVTQQQDKLFYVSPFIEMAMRYHFHVLPPGQCIKLRIL"
BASE COUNT          408 a          709 c          637 g          388 t
ORIGIN      
        1 tctcattcgg catgcggcgc aagcgccccc gttcggaatg gttgctgcaa ggtaagtacc
       61 ggaacacggc cgcgctggcc agagcgccaa ctggaacgcg gtatgatgcg gtgatcgaca
      121 tacccctaaa acgcacgacc acgtcggttg gatcaccggt gatccaaagc cgccagcggt
      181 tcgtttcaca agggttaggc cagatggaag ccgaagtgag gtcttgaatg cgggtcgcgg
      241 tcatcgggac gggaattgcg ggcaatgccg cggcctggag cctgtcgaaa cgttacgcgg
      301 tcacggtcta cgaccgggaa gcgcggatcg gcgggcatag ccacaccgtc gccatcgact
      361 acgacggcgt tccggttgcc gtcgacatcg gtttcatcgt ctacaacgag ctgaactacc
      421 cggacctgac cgcgttgttc gcacatcttg gcgtcgaaac gatcgaaagc tgcatgagct
      481 ttgcggtgac ggcagacacc ggccgcttcg agtggaaagg cggcggcagc aactggctcg
      541 agaccttcag cggcctgttc gcgcaacccc ggaacctgct gtcgccgtcc tatctgcgga
      601 tgctgcgtga cgtcctggcc ttcaacgagc aaagcaccaa agattacgcc gccggcaaac
      661 tcgccgggct gacgctcggc gactatttcc gccgcaacaa cttcgcaccg cgcctgctga
      721 ccgactacct ggcgccgatg ggcgccgcga tctggtcggc accggccagc gagatcctgg
      781 atttcccggc cgagaatttc atcgccttct tctccaacca tcgtctgctg cagtacgacc
      841 gcccggtctg gcgcacggtc aaaggcggca gccaacgcta tgtcgaaaaa ctgaccgcgg
      901 ctttccgcga ccagatcaag cccggctgtg ccgtcaccgc gatcgcgcga acaccacatg
      961 gcgtcctcgt tcacgacagc ctcggcaata ccgacatcta cgatcatgtc attgtcgcct
     1021 cgcatagcga ccaggcgctg gccatgctgt ccgacgccga cgaccacgaa cgttcgatcc
     1081 tgggcgcgat cggctactcc tccaacaagg tctatctgca ccgcgacccc aggctgatgc
     1141 ccaagcgaaa gcgcgcctgg gcctcgtgga atttcctgcg ctggccgcgc aaaggatccg
     1201 tcgataacga cgtctccgtg acctactgga tgaacccgct gcagggcatc gacgcggaca
     1261 agccgctttt cgtcagcctc aacccgccgt tcgaaccgga tccggcgctg accttcggca
     1321 cctacctgtt cgaacatccg cagtacaacg ccgccgcctt tgccgcgcaa aaacgccttg
     1381 gcgaaatcca gggcaagcgc cggacctggt tctgcggcgc ctggaccgga tacggattcc
     1441 acgaggacgg attgcggtcc ggtctggcgg tggcggaagc actcggcgca gtggcgcctt
     1501 ggcgcgaacc ggcgttgagc ctcgcggagg ccgcggagta gcgatgccca aacagattac
     1561 cagcaccgaa cgccacgacg cggctaccga cgccgctgcc gcgctctacg tcggtgacgt
     1621 catgcatgcg cggctgaagc cgataggcca tcgcttcagc taccgcgtca tgagcctgtt
     1681 gatcgacctc gaccggctcg aagaagcgga ccggcagtcg cgcctgttcg gcgtcaaccg
     1741 ggcggcgctt tacagtttca gcgaggctga tcatggcccg cgcgacggat ctccgttgcg
     1801 ggcctatgta cagcactgcg ccgccgagaa cagcatcgac ctcaccggcg ggcgggtgct
     1861 gctgctctgc tatccccgcc tgctcggcta caccttcaat ccgttgtcag tctattttgg
     1921 ctatcgtgcc aatggcgaac tggcactgat gatctacgag gtgcggaata ccttcggcga
     1981 catccatccc tatgtcgtgc cggtgcagcc ggaccaactg agcgatgccg gcgtcacgca
     2041 gcaacaggac aagctgttct acgtctcgcc cttcatcgag atggcgatgc gataccattt
     2101 tcacgttttg ccgccgggtc agtgcatcaa gctgcggatc ct
//
LOCUS       DASWQN010000834         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_476794, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000834 DASWQN010000000
VERSION     DASWQN010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476794"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..937
                     /locus_tag="VGC82_14080"
     CDS             44..937
                     /locus_tag="VGC82_14080"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0424446.1"
                     /translation="MRWAEGPAYFPEGGYLLFSDIPNNRIMKFDEKTGQTTVFRSPSN
                     YANGNTRDRQGRLVTCEHSVTRRITRTETDGKITVLADKFEGKRLNAPNDIVVKSDDS
                     IWFTDPLFGINGEWEGNKEKPEQVTTNVYRIAKDGKITAVITDIVNPNGLAFSPDEKK
                     LYVIEWKGTPNRSIWSYDVNADGTLGSRTKLIDAADQGALDGFRVDRDGNLWCGWGSN
                     GALQSEPTEVGGRKVYQLKGKSEDLDGVMVFNPEGKPLAFIRLPERCANLTFGGPKNN
                     RLYMTACHSVYALYVEAHGAV"
     gene            1005..>1723
                     /locus_tag="VGC82_14085"
     CDS             1005..>1723
                     /locus_tag="VGC82_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HEY0424447.1"
                     /translation="MTKLSDATRSKLKTVSTATVATALFKRGFRIQMIQDVHPLNPDQ
                     PTLVGEAFTLRYMPAREDLNKIDVFRDRAHPQRKAIEDCPPGGVLVMDSRKDARAASA
                     GAILVTRLMKRGVAGVVTDGGFRDSAEIAKLGFAAYHHRPSAPTNLTLHQAIEINVPI
                     GCGDAPVFPGDVILGDSDGVIVIPAHLAEEIANEAFEMTAFEDFVTEQVQGGRSILGL
                     YPATDEQTLVDFAAWRKATGR"
BASE COUNT          350 a          566 c          527 g          280 t
ORIGIN      
        1 gtaccggatc tattcgagca cgatcgagca ggtcgcgacc ggcatgcgct gggccgaagg
       61 cccggcctat ttcccggaag gcggctattt gctgttctcc gacatcccca acaaccggat
      121 catgaagttc gacgagaaga ccggacaaac caccgtgttt cgcagtccgt cgaactatgc
      181 caatggcaac acccgggacc gccagggccg cctcgtcacc tgcgagcatt cggtcacccg
      241 ccgcatcacc cgcaccgaga cggacggcaa gatcacggtg ctggccgaca agttcgaggg
      301 caagcggctg aatgccccga acgacatcgt cgtgaagtcc gacgacagca tctggttcac
      361 cgatccgctg ttcggcatca acggcgagtg ggaaggcaac aaggaaaagc cggagcaggt
      421 caccaccaac gtctaccgca tcgccaagga cggcaagatc accgctgtga tcaccgacat
      481 cgtcaatccc aacgggctgg cgttctcgcc ggacgagaag aagctctatg tcatcgagtg
      541 gaagggcacg cccaaccgca gcatctggag ctatgacgtc aacgccgacg gtacgctcgg
      601 cagccgcacc aagctgatcg acgccgccga ccagggtgcg ctggacggct tccgggtcga
      661 ccgcgacggc aatctgtggt gcggttgggg cagcaacggc gcgttgcagt ccgaacccac
      721 ggaagtcggc ggccgcaagg tgtatcagct gaagggaaag tccgaggacc tcgacggtgt
      781 gatggtgttc aatccggagg gcaagccgct cgccttcatc cggctgccgg aacgttgcgc
      841 caacctcacc ttcggcggac cgaagaacaa ccgcctctat atgacggcct gccactcggt
      901 ctatgcgctc tatgtggaag cccatggcgc ggtgtgagta attaccgaca ttccgggttc
      961 gcgctttgcg cgccccggaa tgacggcaac agggatgata cactatgaca aaactgagcg
     1021 acgccacccg cagtaaactg aaaaccgtct cgaccgccac cgtcgccacc gcgctgttca
     1081 agcgcggctt tcgaatccag atgatccagg acgtgcatcc gctaaacccg gaccagccga
     1141 cgctggtcgg tgaggccttt acgctgcgct acatgccggc gcgcgaggac ctcaacaaga
     1201 tcgacgtgtt tcgcgaccgc gcccatcccc aacgcaaggc gatcgaggac tgtccgcccg
     1261 gcggggtgct ggtcatggac agccgcaagg atgcgcgcgc cgcttccgcc ggcgccatcc
     1321 tggtgacacg gctgatgaag cgcggcgtcg ccggcgtggt caccgacggc ggctttcgcg
     1381 attcggccga aatcgcaaag ctcggttttg ccgcctatca tcaccggccc agcgcgccga
     1441 ccaacctgac cctgcaccag gcgatcgaga tcaacgttcc gatcggctgc ggcgatgccc
     1501 cggtgtttcc cggcgacgtc atcctgggcg attccgacgg cgtgatcgtg atccccgccc
     1561 atctggccga ggagatcgcc aacgaggctt ttgagatgac cgcgttcgag gatttcgtca
     1621 ccgagcaggt gcaaggcggc cgctctatcc ttgggctcta tcccgccacc gacgagcaga
     1681 cgctggtcga tttcgccgcg tggcggaaag ccaccgggcg gta
//
LOCUS       DASWQN010000835         4587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2455962, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000835 DASWQN010000000
VERSION     DASWQN010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2455962"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..677)
                     /locus_tag="VGC82_14090"
     CDS             complement(360..677)
                     /locus_tag="VGC82_14090"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY0424448.1"
                     /translation="MQGLIRPGLLLACLLAPAAARSDNAVQVKIDNFTFSPNKLTIVK
                     GTEVTWENGDDIPHTIVLGAINVRSKTIDTDKTFAYRFDKAGTFSYVCGLHPQMHGQV
                     VVK"
     gene            complement(689..1612)
                     /locus_tag="VGC82_14095"
     CDS             complement(689..1612)
                     /locus_tag="VGC82_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0424449.1"
                     /translation="MNIDDYNADRRGFLKCMTWAGSAMVWTVAGGIPRSHIIGSAGAT
                     EAGFSFAQVSDSHLGFDKPVNTNVAGTFQEALAQVTAAEEKPAFLIHTGDITHLSKPE
                     QFDTAAQLLGATQLQVYTVPGEHDILEDDRKSYLNHYGKGTRGDGWYSFNAHGVHFIG
                     LVNVVNLQGNGLGDLGHTQLEWLENDVKGLSASTPVVVFAHVPLWIVYKDWGWGTVDG
                     AQALSYLKRFGSVTVLNGHIHQVMQKVEGQVAFHTARSTAFPQGVPGVAKGPGPAPVP
                     PGELKNVLGTAKITLVTTKSPLAIVDTPLMG"
     gene            1749..2273
                     /locus_tag="VGC82_14100"
     CDS             1749..2273
                     /locus_tag="VGC82_14100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0424450.1"
                     /translation="MATDGRTRRFELLAMPHLDAAFNLARWLTGNTADAEDVVQDAYL
                     RAFRYLDAFRGDNFRVWLLTIVRNSFLNWLKDNRSGRQLFQPIPADLEWTDPAPSAEA
                     MLLDRVDSETLGVLMAGLPAEYREVLILREIEELSYKDIATVTGIPPGTVMSRLSRAR
                     LALRKAWTESELNR"
     gene            2270..3019
                     /locus_tag="VGC82_14105"
     CDS             2270..3019
                     /locus_tag="VGC82_14105"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY0424451.1"
                     /translation="MNHNHARKLLSAYADHQIGPIRRFWLGRHIAACPSCLAELEALQ
                     ALRSAVRTNLTVHRAPPALAMRIGSALPREAPPSPRRRPISFGFANAAIAGSCAGVAL
                     TLAVTRMTPTPDPLVADVVADHVRSLMADHLTDVATSDQHTVKPWLSARLDLSPVVND
                     FAAQGYPLVGGRLDYVEGHRAAAIVYRRDKHVINLFVFVSDTRTDAPFRQDTRDGFNV
                     VRWGMNGLSYVAVSDVSAPQLLTFAHLVANY"
     gene            complement(3016..3234)
                     /locus_tag="VGC82_14110"
     CDS             complement(3016..3234)
                     /locus_tag="VGC82_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424452.1"
                     /translation="MIAGGSGRVLGFPWAPEQARELPMAPTLLSFRSALALCIVADRG
                     YASHAGSRAWTVSSLTVQAVTPPSMIMR"
     gene            complement(3231..3425)
                     /locus_tag="VGC82_14115"
     CDS             complement(3231..3425)
                     /locus_tag="VGC82_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424453.1"
                     /translation="MWKHLLERAQQRDGIRLGLPFLDATTIPAHRKTAGAAKKAALPH
                     SAAGAKRLAAHVADFPPMPA"
BASE COUNT          866 a         1397 c         1385 g          939 t
ORIGIN      
        1 cgatgaggga atggtggcat tggtaaatgt ccgcacggat taccgtgcgc ggtttgccgg
       61 cgtggcgttc tcggattact cgacttagcg gccgggtatg tggcggccgg agctcgttcc
      121 ggccgccgct atcgcgtttc gggcgggcgt tgaagtcgtg ggtggccggg ccaagcccgg
      181 cgccatgaca cgcgcacatt aacgaccccg ttgacccatc cgcgagaaca ttaacaactc
      241 cttggacgca tccgcgccta cattactaac ctggttgacg cgtctgcggc ttctgctgtc
      301 agcgccgagg gacagtgctt tactgctcgg ctgacgaggc gcctgtatgg ggccgggtct
      361 tattttacta ccacttggcc gtgcatctgt ggatgcagac cgcagacgta ggagaaggtg
      421 ccggccttgt cgaaccggta ggcgaacgtc ttgtcggtgt cgatcgtctt cgaacggaca
      481 ttgatggcgc ccaggacgat ggtgtgcggg atgtcatccc cattctccca ggttacctcc
      541 gttcccttga cgatcgtcag cttgtttggg gaaaacgtaa agttgtcgat cttgacctga
      601 acggcattgt cagatcgcgc agccgccgga gccagcaggc aggcgagcaa aagccccggc
      661 cgaatcaatc cttgcatgaa aactaccctc aacccatcaa aggtgtatcg acgatcgcca
      721 gcggtgactt ggtcgtcacc agggtgatct tggcggtgcc aagcacgttc ttgagttcgc
      781 caggaggcac cggagccggg cccggacctt tggcgacgcc gggcacgcct tgcggaaagg
      841 cggtggagcg cgcggtgtgg aacgcgacct ggccctcgac cttctgcatc acctgatgga
      901 tgtggccatt caatactgta accgaaccga atcgcttcag gtagcttaat gcctgcgcgc
      961 cgtccacggt gccccagccc cagtccttgt aaacgatcca gagcgggaca tgggcgaaca
     1021 ccacgaccgg cgtgctcgcg gacaggcctt tgacgtcatt ctccagccac tccaattgcg
     1081 tgtgacctag atcgcctagc ccgttgccct gcaggttgac cacgttgacc aggccgatga
     1141 agtgcacccc atgggcgttg aagctgtacc agccgtcgcc gcgcgtgccc ttgccataat
     1201 ggttcaggta gctcttgcgg tcgtcctcca ggatgtcgtg ctcacccggg acagtataaa
     1261 cctgcagctg cgtcgcaccc agcaactgtg ccgccgtgtc gaactgctcg ggcttcgaca
     1321 aatgagtgat atcaccggta tggatcagga aagcgggttt ctcttccgcc gccgtcacct
     1381 gggccagggc ttcctgaaaa gtgccggcga cgttggtgtt caccggcttg tcgaagccca
     1441 gatggctgtc gctgacctgg gcaaagctga agcctgcctc ggttgcccca gcgctgccga
     1501 tgatatggga acgtggtatg ccgccggcaa ccgtccatac catggcgctg ccagcccagg
     1561 tcatgcactt caggaagccg cgccggtctg cgttgtagtc gtcgatattc attttgagcg
     1621 ctccttgttg atctcgaagt caataccgag accggaccgc cgatattcct cgttggaaaa
     1681 agatttattg tcgggaataa aacccgccgt cggctggtat aatgaatgct tcatttggga
     1741 tgcgtgtctt ggcgaccgat ggccgaaccc gacgcttcga actgctggcg atgccgcatc
     1801 tggatgcggc gttcaatctc gctcgctggt tgaccggcaa taccgccgat gccgaggacg
     1861 tggtgcagga tgcctatctg cgcgcattcc gctacctcga tgcgttccgc ggcgacaatt
     1921 tccgggtgtg gctgctgacc atcgtgcgca acagttttct caattggctg aaagacaacc
     1981 gctccggccg gcaactgttt cagccgatac cggcggatct ggagtggacc gatccagcac
     2041 cgagcgccga ggcgatgctg ctggatcgcg tggacagcga aacactgggt gtcttgatgg
     2101 ctggattgcc ggcagaatac cgggaggtcc tgatcctgcg cgagatcgag gagctttcct
     2161 acaaggacat cgccaccgtg acggggattc cccccggcac cgtgatgtcg cgtctatccc
     2221 gcgcccggct cgcgctgcgg aaggcatgga ccgagagcga gttgaaccga tgaatcacaa
     2281 ccatgcccgc aagcttctgt ctgcctacgc cgatcatcag atcggcccga tccggcggtt
     2341 ctggctagga aggcacatcg ccgcctgccc gtcctgtttg gccgaactgg aggcactgca
     2401 ggcgttacgg tctgccgtgc gtaccaattt gacggttcac cgcgcaccac ctgcgcttgc
     2461 gatgcgtatc gggtccgcgc tgcctcgtga ggcgccgccg tcgccgcgcc gtcgtccgat
     2521 ttctttcggc ttcgccaacg cggcgatcgc gggtagctgc gcgggtgtcg cactgacctt
     2581 ggcggtgaca cgaatgacgc cgacgcccga cccgctcgtc gccgatgtcg tggccgacca
     2641 tgtccgatca ttgatggccg accacctgac ggacgtagca acaagcgacc agcatacagt
     2701 caagccttgg ctgtccgcac gcttggacct gtcgccggtg gtcaacgatt ttgccgccca
     2761 gggctatcca ttggtcggcg gccgcctgga ttacgttgag ggacaccgtg ccgccgccat
     2821 cgtgtaccgg cgggacaagc atgtgatcaa cctgtttgtc ttcgtatcgg acacgcggac
     2881 ggatgcgccg tttcgtcagg acacgcggga cggtttcaac gtcgtccgct gggggatgaa
     2941 tggcttgtcc tacgtcgccg tttccgatgt ctcggcgccg cagttgctta cgttcgccca
     3001 cttggtcgcg aactattagc gcatgatcat gctgggtgga gttacggcct gcacagtcag
     3061 gctggaaacg gtccaggccc tagatccggc atgcgaggcg tagccgcgat ccgcgacgat
     3121 acaaagtgcc aacgccgaac gaaagctcag cagggtcggt gccatcggca gttcgcgggc
     3181 ttgttcgggt gcccagggaa agccgaggac gcgtccggac ccgcctgcaa tcatgcaggc
     3241 attggtggaa aatccgccac gtgagcggcc aagcgcttcg cgcccgccgc gctgtgcgga
     3301 agagccgcct ttttcgccgc acccgcggtt ttccggtgcg ccgggatcgt ggtggcatcg
     3361 aggaagggca ggcccagccg gatgccgtcg cgctgttgcg cccgctccag caggtgcttc
     3421 cacacgccat ggcggctcca gcggataagg tctgcgccgc catccaccac agccccagat
     3481 gcggcggaac gctgcgccac ttcgcgccgt ttctgtgccg ccagataatc gcttcgatgg
     3541 tgcgccgcag attgagccac tcggccttgt gaggccggca cgctcgatca gcggtgccaa
     3601 ttcctcccat tgcgcgtcgg tcaggacgcc atacccgctt tcctgtgtca tgcctgcaat
     3661 acagggcgga actcggcggc caacagaccc tggtgaaatg ctgctcacga accactcagt
     3721 gcaacccttt tgggcagact gacccgtccc gctggcgccg gtccggcata caccgacatc
     3781 cgtcgcaaaa atcttccgtc agatccgccg ttcgtgcttc aagccccccc tcgcttccaa
     3841 ccgttcgcac acgagcccct tgcgaaccga gacccaacat ggattgcgaa aacatgaaga
     3901 actggactgc atgaacgcta accgcttctt ccgctgcagt ccactccaga ttcagagctt
     3961 gagggaggca tgggcaaatg gcgttcaacc cttcgattat acccgaatag tcaaataatt
     4021 caccgtaatg tggaccaatt tattaattat ccactttgac tgatccggat aagaaaggta
     4081 gtaattgagg cggcggctga cccaaccggg cggcaaagcg acacgagctc gaccgtttga
     4141 gcaattcgga ggggaataat gaagcagtat cgcttactac actttctacc gcttgacctg
     4201 ttaccccaag ttccgtttct actgttggcg ggaaagacac cgcgagggca cgcccagttg
     4261 ccgacagtat atatgtgccc gacggtgtaa taccgaatgc tcggtacccg atggcgtgcc
     4321 agcccaaagg gcttgtccgc acctgggtcc gccttcgtgg tgactacaaa cattatcgcg
     4381 gacgaagtcc ggcagcgtcc catggacgca tcgacgggtc gattccgttc aggcgagcga
     4441 aagctcataa caccccgctt ttgggacgta taaggaggtg tagtcaggta taacgaaccg
     4501 gcgatttggc ttctgagcga ggacgaactg gacctggtgg cggtggtggc ggtggaaccg
     4561 tagtggagat cgtcgagatt tcgatcg
//
LOCUS       DASWQN010000836         4879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1004685, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000836 DASWQN010000000
VERSION     DASWQN010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004685"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VGC82_14120"
     CDS             <1..628
                     /locus_tag="VGC82_14120"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEY0424454.1"
                     /translation="PLVHRPRLPARAQRMLSEIVTGHLLETLAARKDLDPKIELALRS
                     ALARVGPNPTVPPFARSTGSVSTTGQAASDPRAAFPEAEARQRADERDDYAILDALRR
                     KEMMSATTMLAAKAGVAVSVIERACGLGDAKAIVSLAWKAGLTAQTAVVLQVMLAQLP
                     SDRILRPNQGTGYLLSEDEMRSQLASLGTGGAEKRPWMPRRLSATIPQ"
     gene            complement(643..1554)
                     /gene="argB"
                     /locus_tag="VGC82_14125"
     CDS             complement(643..1554)
                     /gene="argB"
                     /locus_tag="VGC82_14125"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY0424455.1"
                     /translation="MSESPSYPTDLAAATQQAHVLAQALPFLRRYAGATVVVKYGGHA
                     MGEEHLAQQFGSDIALLKQVGINPVVVHGGGPQINAMLKRMAIQSTFIDGLRVTDAAM
                     VEVVEMVLAGTVNKYVAGLITKAGALAVGICGKDGGMIRARKLQRTKRDPGSRIEQVL
                     DLGFVGEPTNVDVRVIHALTGGGLVPVIAPVGVDADGQTYNINADTVAAAVAGALNAT
                     RLLMLTDVPGVLDKDKHLIASLTLAEVSERIADGTITGGMIPKVENCVEAVRQGVKGA
                     VILDGRVPHACLLELFTEAGPGTLIRR"
     gene            1710..2684
                     /locus_tag="VGC82_14130"
     CDS             1710..2684
                     /locus_tag="VGC82_14130"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0424456.1"
                     /translation="MRQPQATYAPDGLMAGMVLPKSVSRGRVGMTAGLTNAVLAQLPL
                     AVAVIDAGLRLLYWNEAAAGVFGVPPMLASEMPPLAGILSSVAHLDLPQRDRIMAFVA
                     AEVAAGGRTEPDSCLRVSLGRERRIVLQVRGLSAGRWMLVIDDGRMPVASGRDGAARS
                     DAWLDALTGLSNRRHCNEVLRGMVDGATQAAVLTIDLDRFGPINDTLGHAVGDALLCL
                     VAQRLRRETRDEDLLVRLGSDEFVILLANGARAEALATRVIESLSKPFLVEGHLAEIS
                     ASIGISRFPDHGPTADDLMRHAALALSEAKNDGRRTWRVFAPETALAA"
     gene            complement(2719..2928)
                     /locus_tag="VGC82_14135"
     CDS             complement(2719..2928)
                     /locus_tag="VGC82_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424457.1"
                     /translation="MAIANDEKTAEFQRGWDAAILAARAWHEAKAKQAMVQSRRTRFP
                     KSLERDAEVHRRSAEMMITLRPDDV"
     gene            3185..3562
                     /locus_tag="VGC82_14140"
     CDS             3185..3562
                     /locus_tag="VGC82_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424458.1"
                     /translation="MQGNGVEMDFPNHELTSVPPPEKSRLAGATDNLSRTLTINDITP
                     EHERVLAIIPQRTLQSVASLFERAPKAGRFSALNVAQKLNYIAEVTAVPYRTMKEFSD
                     QPGTLGLMMGRGLARSLEAAPKR"
     gene            complement(3584..>4879)
                     /locus_tag="VGC82_14145"
     CDS             complement(3584..>4879)
                     /locus_tag="VGC82_14145"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0424459.1"
                     /translation="DPAHREAMTFECPDTGDTLGLSFIIPRTQDDLVRRRKMMLNWAR
                     TTCGMMGRSPDFMNVTYACWAGAADYFGRGKPEFADNMRRYYRYIAENDLVLTHSLIN
                     LQRSRNVSGTFNLQEGTALQVVKETSAGIIVRGARVLATLGPLADEIAVYSPRLAQHV
                     EQHSPFALSFALPCDTPGLRFLCRDSFDYEKTHFDHPLGSRFEEMDCVVFFDDVLVPW
                     ERVFILGDVGLLNGAASITHSSAHTAHQGAAKNLAKCELVLGVALLVTETLGNSHLPH
                     AEERIGELVMYTELIRACMRAAEADAELDEWGIMCPAPLPAETTRNLFMTAYPRMVEI
                     LQLLGSSSLMILPTEADLNGPLAGHIDQYLATDNSSARDRVKLFHLAWDISCSSFGNR
                     QVLYERFFASDPLTRARALAGMFPKQQVTDRVLDFLARE"
BASE COUNT          870 a         1553 c         1590 g          866 t
ORIGIN      
        1 gccgctggta caccgcccga ggttgcctgc ccgagcgcaa cgcatgctgt cggagatcgt
       61 gaccggacat ctgctggaaa cgctggcagc acgcaaagac ctggatccca agatcgagct
      121 ggcgttgcgt tcggccctcg cacgggtggg gcctaacccg acagtgccgc cttttgcccg
      181 ctcaacaggt tccgtcagca cgaccggaca agctgcatct gacccgcgcg cggctttccc
      241 ggaggctgag gcgcggcagc gggctgacga gcgcgatgac tacgcgatcc tggatgcctt
      301 gcgtcgcaag gagatgatgt cggcgaccac gatgctcgct gccaaggctg gggttgcggt
      361 ttcggtaatc gagcgggcct gtggtctggg cgatgccaaa gccattgtca gcctggcctg
      421 gaaagccggc ctgacggcgc aaaccgcggt cgtgctgcaa gtgatgttgg cccagttgcc
      481 ctccgacaga atcctgcgcc ccaaccaagg cactggatat ctgctatccg aggatgaaat
      541 gcgttcgcag ctagcgtccc tgggcaccgg aggcgccgaa aagcgtccct ggatgccgcg
      601 ccgactgtca gccaccatcc cgcaatagcc cggcgtgacg gatcagcgcc tgatcaacgt
      661 gcccggcccg gcctcggtga acagttccag caggcacgcg tgcggcaccc ttccgtcgag
      721 gatgaccgcg cctttcacgc cctgacgcac ggcctccacg cagttctcga ccttcgggat
      781 catgccgccg gttatcgtgc cgtcggcgat ccgttcgctc acctcggcca gcgtcagcga
      841 ggcgatcagg tgcttgtcct tatcgagcac ccccggcacg tccgtcagca tcagcagccg
      901 cgtggcgttc agtgccccgg ccaccgccgc ggcgactgtg tctgcattga tgttgtaggt
      961 ctgaccatcg gcatcgacgc cgacaggggc gatcaccggc accaatccgc cgccggtcag
     1021 cgcatggatc acccgcacat cgacgttggt cggctcgcca acgaagccga ggtcgagcac
     1081 ttgctcgatg cggctgccgg gatcccgctt ggtgcgctgc agcttgcggg cgcggatcat
     1141 gccgccgtcc ttgccacaga tgcctacggc cagcgcaccc gccttggtga tcaatccggc
     1201 aacgtatttg ttgacggtgc cggccaggac catctccaca acctcgacca tcgcggcatc
     1261 ggtcactcgc aggccgtcga tgaaggttga ctggatcgcc atccgtttca gcatcgcgtt
     1321 gatctgcggc ccgccgccat gtaccacgac cggattaatg ccgacctgct tcaacagggc
     1381 gatgtcgctg ccgaactgct gcgccagatg ttcttcgccc atcgcatggc caccgtattt
     1441 caccacgacg gtggcaccgg catagcggcg caggaaaggc agggcctgcg ccaggacatg
     1501 cgcctgctgc gtggccgcag cgaggtcggt cggatagctg ggggattcgc tcatgcgcgc
     1561 gtttagggcg ggggccgaaa ggggtcaagc ctcgccgcgg cggcaacgac cggccggtac
     1621 ccggaccgtc agaataaatg ccgggctatt aacgcttcgg taagtacggg cagatactct
     1681 cggccccagg tagtgaactg gaggctgaag tgcgtcaacc gcaggcaacc tatgcaccgg
     1741 atggactgat ggccggcatg gtgctgccga agtcggtttc ccgcggacgg gtgggcatga
     1801 cagccggcct gaccaacgcc gtgctggcgc aactgccgtt ggccgtcgcg gtgatcgacg
     1861 cggggttacg gctgctgtac tggaacgaag ccgccgccgg ggtgttcggc gtcccaccga
     1921 tgttggcttc ggagatgccg ccgctggccg ggatactgtc ctcggtcgcc catctggacc
     1981 tgccgcagcg cgacaggatc atggcatttg tcgcggcaga ggtcgcggca ggcggccgga
     2041 cggagccgga tagctgtttg cgcgtttcgc tgggacggga gcggcggatc gttcttcagg
     2101 tacgcggact gagtgccggt cgctggatgc tggtcatcga cgacggcagg atgccggtcg
     2161 cttccggccg tgacggggcg gcgcgcagcg acgcgtggct cgacgcgctg accggactga
     2221 gcaaccgccg gcattgcaac gaagtcctgc gtggcatggt cgatggcgca acgcaagcgg
     2281 ctgtgctgac gattgatctc gaccgcttcg gacccatcaa cgacacgctg ggtcatgccg
     2341 tgggcgatgc gttgctatgc ctggttgcgc agcgactgcg tcgggaaacc cgggacgagg
     2401 accttctcgt gcgcttaggc agcgacgagt ttgtcattct gctcgccaac ggtgccaggg
     2461 ccgaggccct tgcgactcgg gtgatcgaaa gcttgtccaa gccgtttctg gtcgaaggcc
     2521 acctcgccga aatcagcgcg agtatcggga tctctcgttt tcccgatcat ggcccgacag
     2581 cggatgacct gatgcgccat gcggccctgg ccttgtccga ggcaaaaaac gacggccggc
     2641 ggacctggcg ggtgttcgcg ccggaaaccg cgttggcggc atagcctgtg aagttattcc
     2701 cggcgacgtg atctcgcctc agacgtcgtc cggacgcagg gtgatcatca tttcggctga
     2761 acggcggtgc acctccgcat cgcgttccaa agatttcgga aaccgcgtcc gccgggactg
     2821 gaccatggct tgcttggcct tcgcctcatg ccatgcacgg gctgcaagga tggccgcatc
     2881 ccagccccgt tgaaactccg cggttttttc gtcgttagcg atggccatga gctctcctgg
     2941 ggcatgggtg gaacagaaaa agcggcttcg atacgcgaaa aattgtcgcg atcccgccgc
     3001 ctgcggctaa accattaccg cgccgggccc gacgtccacg gcgcgtgaac tgagcgttca
     3061 gcctcagggg tcggcgagcg tcgaaggaga agcgcgtatg ttttcggttg gacaaatggt
     3121 ttatcaaaag gctggcaaac acagcggcaa ggttttggag tgcgagggcg acagggtcta
     3181 tttggtgcag ggcaacggcg tcgagatgga tttcccgaac cacgagttaa cgtcggtgcc
     3241 tccgccggaa aagagccgac ttgccggcgc aaccgacaac ctgtcgcgaa ccctcacgat
     3301 caacgacatc accccggagc atgagcgggt tctggccatc attccgcaac gaacacttca
     3361 gtcggtggcg tcgttgttcg agcgcgcgcc gaaagccggg cggttcagtg cgttgaacgt
     3421 ggcgcagaag ctcaattaca ttgccgaagt cacggcggtg ccttaccgga cgatgaaaga
     3481 gttcagcgat cagcccggca cgctgggact gatgatggga cgcgggctgg cccgcagcct
     3541 ggaagcggcg cccaagcggt agcgcccgat ccccaccgcc agttcattcc cgtgccagga
     3601 aatccagcac ccgatcggta acctgctgct tcgggaacat ccccgccagc gcccgagccc
     3661 gggtcagcgg atcgctggcg aagaaccgct catacagaac ctggcggttg ccgaacgaac
     3721 tgcaggagat atcccacgcc aggtggaaca gcttcacccg gtcgcgggcg gaactgttgt
     3781 cggtcgccag gtactggtcg atgtgcccgg ccagggggcc gttgaggtcc gcctcggtcg
     3841 gcaggatcat caggctggac gatcccagca actgcaggat ttccaccatc cgcggatagg
     3901 cggtcatgaa caggttccgc gtcgtttccg ccggcagcgg ggcagggcac atgatgcccc
     3961 actcatcgag ttcggcgtcc gcctcggcgg cacgcatgca ggcgcggatc agttcggtgt
     4021 acatcaccag ttcgccgatc cgctcctcgg catgcggcaa atggctgttg ccgagtgtct
     4081 ccgtgaccag gagtgcgaca ccgaggacca actcgcattt cgcgaggttt ttcgcggcgc
     4141 cctgatgcgc cgtgtgggcc gaggaatggg tgatcgacgc cgccccgtta agcaggccga
     4201 cgtcgccgag gatgaacacc ctttcccacg gcaccagcac gtcgtcgaag aacacgacgc
     4261 agtccatctc ttcgaagcgc gaacccagcg gatgatcgaa atgcgttttc tcgtaatcga
     4321 aactgtctcg gcacaggaag cgcaaaccgg gcgtatcgca gggcaacgcg aagctcagcg
     4381 cgaacgggct gtgctgctcc acatgctgcg ccagccgtgg cgaatacacc gcgatctcgt
     4441 ccgccagcgg ccccagcgtc gccaacacgc gcgcgccgcg gacgatgatg ccggcggagg
     4501 tttccttcac cacctgtagg gcggtgccct cctgcagatt gaatgtcccg ctgacattgc
     4561 ggctccgctg caggttgatc agcgagtggg tcagcacgag gtcgttctcg gcgatgtagc
     4621 ggtaataccg ccgcatgttg tcggcgaact ccggcttgcc gcggccgaaa taatctgcag
     4681 cgccggccca gcaggcgtag gtgacgttca tgaagtcggg cgagcgcccc atcatgccgc
     4741 aggtcgtgcg ggcccagttc aacatcatct tgcgccgccg gaccaggtcg tcctgggtgc
     4801 gggggatgat gaacgacaag cccagcgtgt ccccggtatc cgggcactcg aacgtcatcg
     4861 cctcgcgatg cgccggatc
//
LOCUS       DASWQN010000837         3549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1928913, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000837 DASWQN010000000
VERSION     DASWQN010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928913"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3164
                     /gene="rpoC"
                     /locus_tag="VGC82_14150"
     CDS             <1..3164
                     /gene="rpoC"
                     /locus_tag="VGC82_14150"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY0424460.1"
                     /translation="KGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALE
                     LFKPFIYSKLEKYGHATTIKAAKRMVEKERPEVWDILEEVIREHPVMLNRAPTLHRLG
                     IQAFEPVLIEGKAIQLHPLVCTAFNADFDGDQMAVHVPLSLEAQLEARVLMMSTNNIL
                     SPANGKPIIVPSQDIVLGIYYLSLETVLFKETPDNEAPAFGRIGEIEHALFANAITLH
                     DKIRARYETVDAGGNPIKPLVVTTPGRMMIAQILPKHPDVPFDLINRQLTKKSVSDVI
                     DMVYRHCGQKECVIFADRLMGLGFAQAAKAGISFGKDDLIIPESKGTLITSTQAEVKE
                     FEQQYQDGLITAGERYNKVVDAWSRCTDEVAGAMMKEISKQEKGVPTNSVWMMSHSGA
                     RGSPAQMRQLAGMRGLMAKPSGEIIEQPIIANFKEGLSVLEYFNSTHGARKGLADTAL
                     KTANSGYLTRRLVDVAQDCIIVEEDCGTERGLTVKPVMDGGEVVSSLSERTLGRTTAE
                     DVLDPATGKILLANNTLIEEPEAELLETANVEAVKIRSVLTCESDIGVCGHCYGRDLA
                     RGTPVNIGEAVGVIAAQSIGEPGTQLTMRTFHIGGAAQRGAETSSVEASHEGTITVRN
                     RNVIANSTGALVVMSRNCEIVLVDDKQRERARFRVPYGARLLAEENQVVTRGQKLAEW
                     DPFTLPIITERAGKVEYIDLIDSITLVERMDEVTGLTSKVVVDYKQGSKSIDLRPRLQ
                     LKDDKGEVIRLDNNTDARYFLAPDSILSVENGATVQAGDVIARIPREGSKTRDITGGL
                     PRVAELFEARRPKDHAVIAECDGRVEFGKDYKAKRRVIVKNDETGEETEYLIQKGKHV
                     SVQEGDFVRRGDPLVDGPRVPHDILKVLGVEALSDYLVNEIQDVYRLQGVKINDKHIE
                     VIVRQMLQKVEIMDPGDTTFLTGETIDRTEFDSINSKLLKDERPAHAMPVLQGITKAS
                     LQTNSFISAASFQETTRVLTEAATAGKTDSLMGLKENVIVGRLIPAGTGAVMNRLRAV
                     AAGRDQRTLWNENARLTEETAAE"
BASE COUNT          725 a         1058 c         1165 g          601 t
ORIGIN      
        1 tgaagggcaa gcagggccgc tttcggcaga acctgctcgg caagcgcgtc gattattccg
       61 gccgttcggt catcgttgtc ggtccggaac tgaaactgca ccagtgcggg ctgccgaaga
      121 agatggcgct cgaactgttc aagccgttca tttactcgaa gctggaaaag tacggccacg
      181 ccacgaccat caaggccgcc aaacggatgg tggaaaagga gcgtcccgaa gtgtgggaca
      241 tcctggagga agtgatccgc gagcatccgg tcatgctgaa ccgggcgccg acgttgcatc
      301 gcctgggcat ccaggcgttc gagccggtgc tgatcgaagg caaggcaatt cagctccatc
      361 cgctggtctg caccgccttc aacgcggact tcgacggcga ccagatggcg gtgcatgttc
      421 cactgtcgct ggaagctcag ctcgaggcgc gggtgctgat gatgtccacc aacaacatcc
      481 tcagcccggc gaacggcaag ccgatcatcg tgcctagcca ggacatcgtg ctgggcattt
      541 actacctgtc gctggaaacc gtgctgttca aggaaacgcc ggacaacgag gcgccagcgt
      601 ttggccgcat cggcgagatc gagcacgcgc tgttcgctaa cgcgatcacg ctgcacgaca
      661 agattcgcgc gcgctacgaa acggtggatg ccggcggcaa cccgatcaag ccgctcgtcg
      721 tgaccacgcc gggccggatg atgatcgcgc agatcctgcc gaagcatccg gacgtgccgt
      781 tcgacctgat caaccggcag ttgacgaaaa agagcgtctc cgacgtgatc gacatggttt
      841 atcgccattg cggtcagaag gaatgcgtga tcttcgccga caggctgatg gggcttgggt
      901 tcgcccaggc agcaaaggca ggcatctcgt tcggcaagga cgacctgatc atcccggaaa
      961 gcaagggcac gctgatcacc agcacccagg ctgaggtgaa ggaattcgag caacaatacc
     1021 aggacggcct gatcaccgcc ggtgaacgct acaacaaggt cgtcgatgcc tggtcacgct
     1081 gcaccgatga agtggccggc gcgatgatga aggagatcag caagcaggag aaaggcgtcc
     1141 ccaccaactc ggtgtggatg atgtcgcact ctggcgcgcg tgggtcgccg gcgcagatgc
     1201 gccagttggc tggcatgcgc gggctgatgg ccaagccgtc cggcgagatc atcgagcagc
     1261 cgatcatcgc caacttcaag gaaggcttgt ccgtccttga gtacttcaac tctactcacg
     1321 gcgcccgcaa gggtctcgcg gacacggcgt tgaagacggc gaactccggc tacctgaccc
     1381 gacgcctggt ggacgtggcg caggattgca tcatcgtcga ggaagattgc ggcaccgaac
     1441 gcggcctgac ggtgaagccg gtgatggatg gcggcgaggt cgtgtcgtcg ctgtcggaac
     1501 ggaccctggg tcgcaccacg gccgaggacg tgctcgatcc ggcgaccggc aagatcctgt
     1561 tggcgaacaa cacgctgatc gaggagcccg aagcggagct gctggaaacc gccaacgtcg
     1621 aagcggtgaa gatccgttcg gtgctgacct gcgagtcgga tatcggcgtg tgcggtcact
     1681 gctatggccg agacctcgca cgtggcacgc cggtgaatat cggtgaggct gtcggcgtca
     1741 tcgccgcgca gtcgatcggt gagcccggca cgcagttgac gatgcgtacc ttccacatcg
     1801 gcggcgcggc gcagcgtggc gccgagacgt ccagcgtcga agcgagccac gaaggcacga
     1861 tcaccgtccg caaccgcaac gtcatcgcga acagcacggg cgcgttggtg gtcatgtcgc
     1921 gtaactgcga aatcgtcctg gtggacgaca agcagcgcga gcgtgcccgc ttccgcgttc
     1981 cctatggtgc gcgtctgttg gccgaggaga accaggtggt gacccgcggc cagaagctcg
     2041 ccgaatggga cccgttcact ctgccgatca tcaccgagcg tgccggcaag gtcgaataca
     2101 tcgacctgat cgacagcatc acgctcgtgg aacggatgga cgaggtaacc ggcctgacct
     2161 ccaaggtcgt cgtcgattac aagcaggggt ctaagagcat cgatctgcgt ccccgcctgc
     2221 agttgaagga cgacaagggc gaggtcatcc gcctcgataa caacaccgac gcccgttact
     2281 tcctggcgcc ggattcgatc ctgtcggtgg aaaatggggc cactgttcag gccggcgacg
     2341 tgatcgcacg tatcccgcgc gagggcagca agacccgtga catcaccggc ggtctgccgc
     2401 gggtggcgga actgttcgag gctcggcgtc ccaaggacca tgcggtcatc gccgagtgcg
     2461 acgggcgggt ggagtttggc aaggactaca aggcgaagcg ccgcgtcatc gtgaagaacg
     2521 acgagaccgg cgaggaaacc gagtacctga tccagaaggg caagcatgtg tccgttcagg
     2581 agggggattt cgtccgccgc ggcgacccac tggtcgatgg gccgcgcgtg ccgcacgaca
     2641 tcctgaaggt gctgggtgtg gaagcgttgt ccgattactt ggtcaacgag atccaggacg
     2701 tttatcgact gcagggcgtg aagatcaacg ataagcatat cgaggtgatc gtccgtcaga
     2761 tgctgcagaa ggtggagatc atggatccgg gcgacaccac cttcctgacc ggcgagacga
     2821 tcgatcgcac cgagttcgac tcgatcaaca gcaagctgct gaaggacgag cgtccggcgc
     2881 atgcgatgcc ggtgcttcag ggcatcacga aagcctcgct gcagaccaac agcttcatca
     2941 gcgcggcgtc gttccaggag accacgcggg tgctgaccga ggctgcgact gcgggcaaga
     3001 ccgacagcct gatggggctg aaggagaacg tgatcgtcgg ccgtttgatc ccggccggca
     3061 cgggtgcggt gatgaaccgc ctccgcgccg tggccgccgg ccgcgaccag cggacgcttt
     3121 ggaacgagaa tgctcggctg acggaggaga ctgcggcgga gtaacgtccg cgcatttggg
     3181 atgacggagg gccggtggta cgagagtgcc atcggccttt tttgttacct acggccgtag
     3241 atgtttttgg gatctgcgtg cagccacgat tgcaggcatc aagaccgccg cgatcattcg
     3301 atgcaccaca aaggccagcg catctcggtg ccttcggatt gatgggtggc gatgagaccc
     3361 gctgcgcagg ccgcgcaggt aggcgctgca ccgactgagg ccgatataaa tcagatcgcg
     3421 cttgatagac ggtaccgccg ccccttacgt cgctgccaat gcccgccgca atccctccgg
     3481 atcccccttg atcgcccgca gcaacgtgcg agctggacca ctcggccgtt tggcgcctcg
     3541 ctcccattc
//
LOCUS       DASWQN010000838         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1664990, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000838 DASWQN010000000
VERSION     DASWQN010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664990"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VGC82_14155"
     CDS             complement(<1..965)
                     /locus_tag="VGC82_14155"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HEY0424461.1"
                     /translation="MKLPADHPQRIELNDEVHARPPEPLVAPCRLSYLALLSDAAQRE
                     AGWLAVCDLCRRYGVAPPAGRPLHFSAELGPFRLKWERHTEFIRFMFIVEGTTGDPFE
                     RPAISVLPEDWVAGLPGEIMVAAHAALIKADPGTVDPQAIGNRWFAGNMPAGAAVSGG
                     AATALTDFRIHADGFSRHLLLDHRTRPWQAGRVAQGLLEIDTYRVMALLALPVARAVG
                     PLLATSEQELSQITASLVSASETDEPALLDRLTQLEAEIENQQSKTRYRFGAADAYYE
                     LMRHRIDELREQRIQGLQAFREFTERRLAPAINTCRSIAVRQKSLS"
     gene            complement(949..1833)
                     /gene="bchO"
                     /locus_tag="VGC82_14160"
     CDS             complement(949..1833)
                     /gene="bchO"
                     /locus_tag="VGC82_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase BchO"
                     /protein_id="HEY0424462.1"
                     /translation="MSERLNWGRDGQDWPHRRFSRFIEAEGLTWHVQVIGNGPAVLLI
                     HGTGASTHSFRSLVPLLANDFTVVVPDLPGHGFTGTPLLASGFTLPRVARGMAALLRE
                     LGLKPVVVAGHSAGAAIAVRMTLDRSITPVAVVSLNGALLPFRGVANDLFGPAARFLA
                     SSTMTARAFTFFAGSRPSVERLVRSTGSHIDEEGVRLYARLAASSVHVHGALALMANW
                     DLRPMARDLPHLPAHLVLVVGSRDGMVPPSESYRVGAMVPNAELVSLRGLGHLAHEER
                     PAEIAALLQRAVTHETAG"
     gene            complement(1830..>2449)
                     /locus_tag="VGC82_14165"
     CDS             complement(1830..>2449)
                     /locus_tag="VGC82_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0424463.1"
                     /translation="SDRFQVRRDDFRIVRFRQRIGTTTIFAVDASGSAAMHRLAEAKG
                     AVELLLADCYVRRDQVAMIAFRGDGATLLLPPTGSLLRAKRCLAGLPGGGPTPLATGI
                     AAAHGLAGSIRRAGRTPVITLLTDARANIARDGTGGRPRAEAEALEIGRSLRATRVAT
                     LLVDTSPRPNPFARRLAEEMGARYVPLPYADASMLSRAVRSTASA"
BASE COUNT          474 a          831 c          771 g          373 t
ORIGIN      
        1 gataacgatt tctggcgcac cgcgatcgaa cggcaggtgt tgatcgccgg agccagccgg
       61 cgttcggtaa attcccggaa agcctgcagg ccctgtatgc gttgctcgcg cagttcgtcg
      121 atcctgtgcc gcatcaactc gtaataggca tccgccgccc cgaaccggta tcgggtcttc
      181 gattgttgat tttcgatttc cgcctcaagc tgggtcaacc ggtccaacag cgccggctca
      241 tccgtttcgc tggcggaaac cagcgacgcg gtgatttgcg acaactcctg ttcggatgtg
      301 gccagcaacg gtccgacggc tcgcgcaacg ggtaacgcca gcaaagccat cacgcggtag
      361 gtgtcgattt ccagaagccc ttgcgccacc cgcccggcct gccacggcct cgtgcgatga
      421 tccagcagca aatgccggct gaacccgtcg gcatggatgc ggaaatccgt aagcgcggtc
      481 gccgccccgc cggagaccgc cgcgccggcg ggcatgttgc cggcaaacca gcgatttccg
      541 attgcctgcg gatcgacggt tcccggatct gccttgatca gcgcggcatg ggcggcgacc
      601 atgatttctc ctggcagtcc cgctacccaa tcctcgggca acaccgagat cgccggccgc
      661 tcgaacggat cgccggtggt gccttcgacg atgaacatga atcggataaa ctcggtgtgc
      721 cgttcccact tcaggcgaaa cggccctagc tcggcgctga agtgcaacgg gcgtccagcg
      781 ggcggtgcca cgccataacg ccggcacagg tcgcacaccg caagccatcc ggcttcgcgt
      841 tgggctgcgt cgcttagtaa ggccagatag gacagtcggc acggtgccac cagcggctcc
      901 ggcgggcggg catgtacctc gtcgttcagt tcgatccgct gggggtgatc agccggcagt
      961 ttcatgcgtg accgctcgtt gcagcaaggc ggcgatctcg gccggccgct cctcatgcgc
     1021 aagatggccc aacccgcgaa gcgacaccag ttccgcgttc ggcaccatgg cgcctacgcg
     1081 ataggattcg gacggcggaa ccataccatc ccggctgccg accaccagca ccaggtgggc
     1141 aggcaaatgc ggcaggtcac gcgccatcgg ccgcaggtcc caattcgcca tcagcgccag
     1201 cgcgccatgc acatgaaccg aactggctgc caagcgggca tacagacgca cgccctcctc
     1261 gtcgatgtgc gatccggtgg atcgcaccag ccgttccacc gacgggcgag acccggcaaa
     1321 gaaagtaaat gcccgggccg tcatggtgga gctggccaaa aaccgggcgg cagggccgaa
     1381 taaatcgttg gccaccccac ggaacggcag cagcgcgccg ttcagactga ccacggcgac
     1441 aggggtgatg gaccgatcca acgtcatccg cacggcgatg gcggctcccg cagagtgacc
     1501 cgccacgaca accggcttca gcccaagttc ccgcaacaga gccgccatcc cgcgcgccac
     1561 ccgaggcaac gtaaacccgg acgccaataa tggagtcccg gtaaatccgt gccccggcag
     1621 atcgggcaca acgaccgtga aatcattcgc caacaagggg acaagggagc gaaacgaatg
     1681 agtggaggct ccagtgccgt ggatcagcag caccgctggt ccgttgccta tcacctggac
     1741 atgccaggtc aggccttcgg cttcgatgaa acggctgaac cggcgatgcg gccagtcttg
     1801 tccatcacgt ccccagttca atcgctcgct cacgccgacg ccgtggaccg caccgctcgg
     1861 gatagcatcg aggcgtcggc ataaggcaac ggcacatacc gggcacccat ctcctcggcc
     1921 aagcgccgag cgaaggggtt gggccgtggc gaggtatcga ccagcagtgt tgccaccctc
     1981 gttgcccgca gcgagcggcc aatctccaac gcctccgcct cggcgcgcgg ccggccgccg
     2041 gtgccgtcgc gggcaatatt ggcgcgggcg tcggtcagca acgtgatcac cggggtgcgg
     2101 ccggcacggc gaatgctgcc ggccagtcca tgcgcagccg cgatcccagt cgcgagcggc
     2161 gtggggccgc cgccgggcag acccgcgagg caacgtttgg cccgcagcag cgatccggtc
     2221 gggggaagca gaagagtcgc gccatcgcct cggaacgcga tcatcgccac ctggtcgcgc
     2281 cggacatagc aatcggcaag caggagttcg accgcgcctt tcgcttccgc taaccggtgc
     2341 atggcggcgg agcccgacgc atccaccgca aaaatcgttg tggtgccgat gcgctggcga
     2401 aaacgcacga tgcggaaatc gtcacgccgg acctggaacc gatccgaac
//
LOCUS       DASWQN010000839         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1797186, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000839 DASWQN010000000
VERSION     DASWQN010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797186"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2096
                     /locus_tag="VGC82_14170"
     CDS             <1..2096
                     /locus_tag="VGC82_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424464.1"
                     /translation="LDRFESQARQHPILLLMEDTQWADDTTLELIDLIVERARQLPML
                     VLVTFRPEFEPPWAGLANVGALSLGRLGRESVESIVAQVTSGRALPSEVTKQIVAKTD
                     GNPLFVEELTKAVLEAGILVEYPEGYRLDGPLPPLAIPATLQDSLMARLDRLLSVKEI
                     AQTGAAIGREFSYPVLRAVVARDDAALGHALTELEQAELVYRRGTPPEAVYSFKHALV
                     RDAAYESMLKSRRQQLHGQIARALENGFADVAASEPEILAHHFTEAGTVQRAITYWLK
                     AGNLALSRSSNSEAVKHLSRGIELIQNLEPSPERTRKELDFYLALGPAVAATEGDAAQ
                     ETSRVFSRARDLLGDGGTPTEQMTVLWGNYLAHSMRAEYSAAVEVARQCLTLASEHQH
                     PGMLALASRFMGQTLHFMGAFAEARAHLERTLAICAANQETIATYRRFGTDDQVTTLS
                     FLAVTLFLLGYPEQSAVVADQAISRARTIGLAFTTAVALSNMAVIGTIGGDARRALAE
                     ADEAIAISADNEFASTDHRARFFRGALSAQGGDHQLGIELMQNAMAAAEHTSERNRHT
                     LYLCHIASAHASLGRPDVALGLLDEALQIAETTSEKFFLAELYRLRGKILLGLGKKAE
                     AEAELRVGLLIAQQQQARWWELRVAATLAEHLRDEGRSAEACSVLKPVFDWFSEGFDT
                     PDLKHAKALLDQLGC"
BASE COUNT          426 a          685 c          678 g          396 t
ORIGIN      
        1 tgctcgatcg gttcgagagt caggcgcgcc agcacccgat cctgttgctg atggaagaca
       61 cgcagtgggc cgacgacacc accctcgagc tgattgacct gatcgtcgag cgggcgcgcc
      121 agctgccgat gcttgtactg gtcaccttcc ggcccgagtt cgagcccccc tgggccggtc
      181 tggccaatgt cggcgccttg tcgctcggca ggctcggccg tgagagcgtt gaaagtatcg
      241 tcgcccaggt gaccagcggc cgcgcgctgc ccagcgaggt gacaaaacag atcgtcgcca
      301 agaccgatgg aaatccatta ttcgtcgagg aactcaccaa ggcggttctc gaagcaggga
      361 tcctggtcga ataccccgag ggctatcggc tcgatgggcc gcttccgccc ctcgcgattc
      421 cggccacgct tcaggactcc ttgatggcac gacttgaccg cctgctttcg gtaaaggaga
      481 tcgcccaaac cggcgccgcg ataggccggg aattctccta tcccgtgttg cgcgctgtgg
      541 tcgcgcgcga cgatgctgca ttgggacacg ccctcaccga actcgagcag gccgagctgg
      601 tgtaccgccg cggcacgccg ccggaggcgg tctacagctt caagcacgcg ctggtgcgag
      661 atgcggccta tgaaagcatg ctcaagagcc ggcggcagca gctgcacggc cagatcgcac
      721 gcgcattgga gaacggcttt gccgatgtgg cagcgagcga accggaaatc ctcgcgcacc
      781 atttcacgga ggccggcacc gttcagcgcg ccatcaccta ctggctcaag gcaggaaatc
      841 tggcgctgag ccgctcctcg aactctgaag ccgtcaagca tctcagccgg gggatagagc
      901 ttattcagaa tttggaacct tcgccggagc gcacccgcaa ggaactggat ttctacctgg
      961 ctctcggacc ggcagttgcg gcgactgaag gcgatgccgc acaggaaacg tcgagagtgt
     1021 tctcacgcgc tcgcgacctg ctcggcgatg gcgggacccc gacagaacag atgacggtgc
     1081 tctggggcaa ctacctcgcc cacagcatgc gggcggaata tagcgccgcc gtcgaagtcg
     1141 cccgacaatg cctgacgctg gcgtcagagc atcagcatcc ggggatgttg gccctggcca
     1201 gccggttcat gggccagact ttgcatttca tgggagcttt cgctgaggcg cgcgcgcacc
     1261 tggagcggac gctggcaata tgcgcggcca atcaggagac gattgcgacc tatcgccgtt
     1321 ttggcactga cgatcaggtt accacgctgt cctttctcgc cgtcacgctt ttccttctcg
     1381 gctatccgga gcagtccgcc gtcgtcgccg accaggcgat ctcacgcgcg cgaacgattg
     1441 gactggcttt cacgactgcc gtggcgctga gcaatatggc cgtcatcggg acgataggcg
     1501 gcgatgcccg gcgcgcgctg gccgaggccg atgaagccat agccatcagc gccgacaacg
     1561 aatttgcaag taccgatcac agggcacgtt ttttccgggg ggccctgtct gcgcaaggcg
     1621 gtgaccacca gctaggcatc gagctgatgc aaaacgccat ggcggcggcc gaacacacct
     1681 cggaacgaaa ccggcacact ctttatctct gccatatcgc ttctgcccat gcgagcctcg
     1741 gccggccgga cgtcgcgctc ggtttgttgg acgaagcgct gcagatagcc gaaacaacaa
     1801 gcgagaaatt cttcctggcc gaactttatc gtctgcgagg caagattctg ctgggtctgg
     1861 ggaagaaggc cgaggcggaa gctgaattgc gcgtggggtt gctgatcgcg cagcagcagc
     1921 aagcacgttg gtgggaactg cgcgtcgccg ccactcttgc ggagcatttg cgcgacgagg
     1981 gcagatcagc ggaagcctgc tctgtcctca agccggtttt cgattggttc tccgaaggtt
     2041 tcgacacacc ggatctgaag cacgccaaag cgctcctgga ccaattgggc tgctagccag
     2101 caaactcaat cgatccagga atggcgaaca ataacggcag gcaccacccg ccgcctcagc
     2161 cctgatgcct ttggaactag gcttt
//
LOCUS       DASWQN010000840         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1136477, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000840 DASWQN010000000
VERSION     DASWQN010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136477"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VGC82_14175"
     CDS             complement(<1..240)
                     /locus_tag="VGC82_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0424465.1"
                     /translation="MTTMHPRDTGTEATRLLEVSGLKKHFPIYKGVFARPSGQVYAAD
                     GVSFHIDRGETLGLVGESGCGKSTVGRTLLKLIEPT"
     gene            complement(237..1289)
                     /locus_tag="VGC82_14180"
     CDS             complement(237..1289)
                     /locus_tag="VGC82_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0424466.1"
                     /translation="MIRPTDIPEAATGATDTEPVLEIDNLQTYFFTPAGIVRAVDGVS
                     YAVKPGQTLGVVGESGSGKSVTALSILRLVAKPPGRIVGGSVRFRGTELLNLSETDME
                     AVRGNRISMIFQEPMTSMNPLLKVGHQISEAIALHQGLSRRAAMDQATEMLRRVHIPE
                     PERRAHAYPHQLSGGMRQRAMIAMALSCNPQVLLADEPTTALDVTIQAQILDLIRELQ
                     DASGMAIVLITHDMGVIAENADRVVVMYAGRKVEEADVDDIFDNPGHPYTKGLLGSIP
                     HLDGARQRGTSRRPRLNEIKGMVPSLAALPPGCSFAPRCGMASEPCRDTYPPLVQHRP
                     GHWVACWHADRVLAGA"
     gene            1429..>2048
                     /locus_tag="VGC82_14185"
     CDS             1429..>2048
                     /locus_tag="VGC82_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424467.1"
                     /translation="MNDADSARRNATRREFMKVSAAVAAGMYGLAPRYGLAADIPMQF
                     DGSKFQLAAPEPNPKRGGVLRYGITNRLPHFDVHQSGTINSLGAQGCMFDNLIRRDPR
                     DSGKTIIPDLAESWEIAPDGKTYTFFLRKGVLFHDGAELTAADVKATFDRIAKPPPGI
                     AIPRSILFATVSEINARDKYTVEFKLSEPRPANFIMSAIASGWNVIV"
BASE COUNT          400 a          627 c          622 g          399 t
ORIGIN      
        1 agtcggctcg atcagcttga gcaacgtgcg cccgactgtg gatttgccgc agccgctctc
       61 accgaccagc ccgagggttt cgcctcggtc gatatggaac gagacgccat cggccgcata
      121 tacctgcccc gaaggtcgcg caaagacgcc cttgtaaatc ggaaaatgtt ttttgaggcc
      181 actgacctcc agcagcctgg ttgcttcagt gcccgtgtcc ctggggtgca tggtggtcat
      241 gcgccggcga gcactcggtc cgcatgccag caggccaccc agtgaccagg acggtgctgc
      301 accagcggcg ggtaggtgtc ccggcaaggt tcgctcgcca tgccgcaacg gggggcgaag
      361 ctgcaccccg gtggcagcgc agccaatgac ggaaccatac ccttgatttc gttcaaccgc
      421 gggcgtcggg atgtcccccg ttgtcgggca ccgtcgaggt gcgggatcga accaagcaga
      481 cctttggtat agggatggcc cgggttgtcg aagatgtcgt caacgtcggc ttcctccacc
      541 ttgcgtccgg cgtacatcac cacaacgcgg tcggcgttct cggcgatcac gcccatgtcg
      601 tgcgtgatca ggacgatcgc catgccggaa gcatcctgca actcccgtat caggtcgagg
      661 atctgcgcct ggatggtgac gtccaaagcc gttgtcggct cgtcggccag cagcacttgc
      721 ggattgcagg acagcgccat ggcgatcatc gcgcgttgac gcatgccgcc ggaaagctga
      781 tgcggatagg cgtgcgcgcg gcgctccggt tcgggaatat gaacccgccg gagcatttcc
      841 gtggcttgat ccatggccgc gcggcgagac aggccttggt gcaaggcgat cgcctcgctg
      901 atctgatgcc caaccttgag caacgggttc atcgaggtca tcggttcctg gaagatcatc
      961 gaaatccggt tgccgcgcac ggcttccata tccgtctcgc tcaggttcaa cagctcggtt
     1021 ccgcgaaagc ggaccgatcc cccgacgatc cgcccaggcg gcttggcgac caggcgcagg
     1081 atggaaagcg cggttacgct tttgccgcta ccggattcgc cgacgacccc taatgtctgt
     1141 ccgggcttca ccgcatagga aacaccatcc acggcccgca cgatcccggc gggcgtgaaa
     1201 aaataggtct gaaggttgtc gatctccagg accggttcgg tatcggtcgc acccgtggcc
     1261 gcctccggaa tatcggtggg tcgaatcaat gcaggctccc tgtccggtac attatggtcc
     1321 ggcatattat ttttaacgtg catcatggta tgaattgtca ggggagcata gggtcccctg
     1381 cgttgaacag atcaaatcaa tagatcgacg atcgggagga atggaaaaat gaatgatgcg
     1441 gactccgcac gccggaacgc gacccgccgg gagtttatga aggtcagcgc cgccgttgcg
     1501 gctgggatgt acggcttggc gccgcgttat gggctggccg ccgacatccc aatgcagttc
     1561 gacggctcca agttccagct tgcggcaccc gaacccaatc caaagcgtgg cggtgtgctg
     1621 cgttacggca tcaccaaccg gctgccgcat ttcgatgtgc accaatccgg taccatcaac
     1681 agtctgggcg cccagggctg catgttcgac aacctgatcc gccgcgaccc gcgcgacagc
     1741 ggcaagacca tcattcccga tttagccgaa agctgggaga tcgccccgga cggcaagacc
     1801 tacactttct ttctgcgcaa aggcgtgttg tttcatgacg gtgcggaatt aacggcggcg
     1861 gatgtaaagg cgacattcga tcggattgcc aagccgccgc cgggaatcgc cattccccgc
     1921 agcatcctgt tcgccacggt ttctgaaatc aatgcccgcg ataagtacac cgtcgaattc
     1981 aagctgagcg aacctcgtcc ggcgaacttc attatgtcgg cgatcgccag cggctggaat
     2041 gtgatcgt
//
LOCUS       DASWQN010000841         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2456372, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000841 DASWQN010000000
VERSION     DASWQN010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2456372"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VGC82_14190"
     CDS             complement(<1..208)
                     /locus_tag="VGC82_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424468.1"
                     /translation="MVKSLRRHWGTLLPMLAIWPPLILSAGHVVVFTLWTAWLSFTPS
                     TLLPEGGFVGWRNYTSVMATRNFHV"
     gene            complement(287..1564)
                     /locus_tag="VGC82_14195"
     CDS             complement(287..1564)
                     /locus_tag="VGC82_14195"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424469.1"
                     /translation="MADLSRRTLMTATGAALAAPNALRAENVKPRLTAISQWSTGSDG
                     AAISALGKLFEQNGGLWQHSPVPGFTTEMMNKLRAQIMSGDPPAASQLKGPEIAAWSK
                     IAPTVDLDAIVAEAGYDKVVAPELAALHKPFGHWIALPLQLYRINTLFVSKKAMDKVG
                     ATALPATWAEFNGLATKMKAAGIIPLANGGIRYDDGMKWEIALNGISPAIYRKAIMEL
                     DTDALNSPEVLGAFEQTRKLADWMNPSIAAQGWSVQVAPFIRGEMGMLLMGGWAQGNI
                     INGGFTLADFVSGPAPQDNGRAAYIMNADAFILWQRKEADLQAGQRLFAKLVMQLSTQ
                     EMYSHITGSIPVRTDADLSGPGWSDQQRSSAQGLKDALKEKQVVLSLAHNMAQPNPIT
                     AAMIDVLTEFVHNKDLKPKEAQQRLVDSIDSAR"
     gene            1741..2829
                     /locus_tag="VGC82_14200"
     CDS             1741..2829
                     /locus_tag="VGC82_14200"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY0424470.1"
                     /translation="MKITSIAMQEYRWPRSKPITNGKHTYTHVDYALVKIGTDQGITG
                     IGLGHGGEIERAIIAHLAPMLVGEDPINVERLWHKMWVPKLVGRRGLTTRAISAIDIG
                     LWDIRAKVANLPLWQMLGGFRDRVPTYVAGGYYEEGKGLHELAREMEDNVRMGARAVK
                     MKVGGAPIAEDAERVRVARDAVGPGVKLLVDANCAYRSYEAIQFARKIEPYDVFWFEE
                     PVMPDDYDGHRKLAQATSIPIATGENEYTRYGFRDLIRNDCAAILNADAKILGGVTEF
                     MKVAALAQAHDLDIAPHGSQDIHVHLVAAISNGLILEFYRDTVDPMWGRIYHQTLKLN
                     DDGTVSAPLAPGIGIEPNEDVLAPYRIA"
BASE COUNT          557 a          956 c          966 g          542 t
ORIGIN      
        1 caacgtggaa attccgcgtc gccatcaccg aggtatagtt gcgccagccg acgaacccac
       61 cttccggcag caacgtcgaa ggcgtgaacg acagccacgc cgtccacagc gtgaacacca
      121 cgacatgccc ggccgaaagg atcagcggcg gccagattgc cagcatcggc aacagcgttc
      181 cccagtgccg acgcagcgac ttgaccacgc ggcggccgga cgcatcctgc cggaggtgcg
      241 gggctctcaa tatgccgctg ggggcgccgt cgctgggcaa cgcgtgtcag cgcgcgctgt
      301 cgatcgaatc gaccagccgc tgctgcgcct ccttcggttt caggtccttg ttgtggacga
      361 actcggtcag gacgtcgatc atcgccgcgg tgatcgggtt cggctgggcc atgttatgcg
      421 ccaggctcag caccacctgt ttctccttca atgcgtcctt cagtccctgg gcgctgctgc
      481 gctgctggtc cgaccagccc gggccgctga ggtcggcatc ggtgcgcacc gggatggagc
      541 ctgtgatgtg cgaatacatc tcctgcgtgg aaagctgcat caccagcttg gcgaacagcc
      601 tttgtcccgc ctgcagatcc gcctcttttc gttgccaaag gatgaaggcg tcggcattca
      661 tgatgtaggc ggcgcggccg ttatcctgag gcgccggccc gcttacaaaa tcggccagcg
      721 taaagccgcc gttgatgatg ttgccctgcg cccaaccgcc catcagcagc atccccattt
      781 cgccgcggat gaagggcgcc acttgcacgc tccagccctg tgcggcgatc gatgggttca
      841 tccaatccgc cagcttgcgg gtctgctcga aggcgccgag cacttcgggg ctgttgaggg
      901 cgtcggtgtc cagctccatg atcgccttgc gatagatggc ggggctgatg ccgttcagcg
      961 cgatctccca cttcatgccg tcatcataac ggatgccgcc gttggcgagc gggatgattc
     1021 cggccgcttt catcttcgtc gcaagcccgt tgaattccgc ccatgttgcc ggcagcgcgg
     1081 tagcgccgac cttgtccatc gccttcttcg agacgaacaa cgtattgatg cggtacagct
     1141 gcagcggcaa cgcgatccag tggccgaacg gcttgtgcag ggcggcgagt tccggcgcca
     1201 cgaccttgtc gtagcccgcc tcggccacga tcgcatcaag gtccacggtc ggggcgatct
     1261 tcgaccatgc cgcgatctcc ggccccttga gttgtgacgc ggccggcgga tcgccggaca
     1321 tgatctgcgc ccgcagcttg ttcatcattt ccgtggtgaa gcccggcacc gggctatgct
     1381 gccagaggcc gccgttctgc tcgaacagct tgccgagcgc cgagatcgcg gcgccatcgc
     1441 tgcccgttga ccattgcgat atcgccgtca gtcgcggttt gacgttctcg gcacgcaagg
     1501 cgttcggtgc cgccaacgcc gcgccggtgg cggtcatgag ggttcggcga gacaggtcgg
     1561 ccatggtttc ctccggtgcc ggacgcttcc cggcgcccat tgcagccagc ttagccaacc
     1621 cctcgaccgg cgtcacctgt ggacaaaagc caacggctga tgtcctttcg gaggaaatcg
     1681 gcgctgcgct accaagcggt gccagtaccg gacgaagatc atgagcaagg aggaaaaccc
     1741 gtgaaaatca cctcgatcgc gatgcaggag tatcgctggc cgcgcagcaa gccgatcacc
     1801 aatggcaagc acacttatac gcatgtggac tacgcgctgg tgaagatcgg caccgaccag
     1861 ggcataacgg gaatcggtct cggccatggc ggcgaaatcg agcgcgccat catcgcccat
     1921 cttgcgccga tgcttgttgg cgaggatccg atcaacgtcg aacgcctgtg gcacaagatg
     1981 tgggtgccaa aactggttgg ccgccgcggc ctgaccacgc gagcgatctc ggcgatcgac
     2041 atcggattgt gggacatccg cgccaaggtc gcgaacctgc cgctgtggca gatgctgggc
     2101 ggcttccgcg accgcgtgcc gacctatgtc gccggcggct actacgagga aggcaagggc
     2161 ctgcacgagc ttgcccggga gatggaggac aacgtcagga tgggcgcccg cgccgtgaag
     2221 atgaaggtgg gcggcgcgcc gatcgccgaa gacgccgaac gcgtgcgggt tgcgcgtgac
     2281 gccgtggggc caggggtgaa actgctggta gatgcgaact gtgcctatcg gtcctacgag
     2341 gcgattcagt ttgcccgcaa gatcgagccg tacgacgtgt tctggttcga ggaaccggtg
     2401 atgcccgacg attacgacgg ccatcgaaaa ctggcacagg cgacatcgat cccgatcgcc
     2461 acgggcgaga acgaatacac ccgctatggc ttccgcgacc tgatccgcaa cgactgcgca
     2521 gcgatcctga acgcggacgc caagatcctc ggcggcgtca ccgagttcat gaaggtcgcg
     2581 gccttggccc aggcgcacga cctggacatt gcgccgcatg gttcgcagga catccacgtg
     2641 catctcgttg cagcaatttc gaacgggctg atcctggagt tctaccgcga cacggtcgat
     2701 cccatgtggg gccggattta ccaccagaca ctgaagttga acgacgatgg cacggtcagc
     2761 gcaccgctgg cgccgggcat tggcatcgag ccgaatgagg acgtgttggc cccctatcgg
     2821 attgcgtgag cggcaggaaa ggagacggca aatgaagatc accaaggtcg agccgctgct
     2881 gcttgatcgt ttctgttacg tgcgcatcga aaccgaccag ggcatcaccg gcctgggcga
     2941 gagcggcgcc tgggggcagc ttgaggcgac ggccgcctcg atttcgaaat acgccgaata
     3001 cctgattggc aaggacccgc g
//
LOCUS       DASWQN010000842         6169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_477254, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000842 DASWQN010000000
VERSION     DASWQN010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477254"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VGC82_14205"
     CDS             <1..840
                     /locus_tag="VGC82_14205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0424471.1"
                     /translation="RSTFCTELSFKDHMTKAIPAGDSHDASEKDREIEELREAVRELR
                     EAVRARDEFVAIAAHELRNPMTPILMQVDMLMNAAKQPGRCRPEIIIPRLERLEFAVG
                     EFIRRSTTLLDVSRIAAGNLQIELSKFDLSQVVREVVARAEPSARMTRSHVEADLQEQ
                     VVGTWDRLAIEQVAENLLSNALKFGAGKPVTVTVRRDGRTVQLVVRDQGIGISEEASA
                     RIFQRFERAVGHSEHGGFGIGLWLANQLVVAMGGTIDVQSRPGEGATFLVNFAHAAAR
                     VIQ"
     gene            837..2318
                     /locus_tag="VGC82_14210"
     CDS             837..2318
                     /locus_tag="VGC82_14210"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY0424472.1"
                     /translation="MNEIRTIERVPSGVPGLDTILNGGFTKSGIHILEGPPGAGKTTL
                     ANQICYSHAAAGGVALYVTLLAETHTQLLLNLATMRFFDLSLLPERISYISGFGAMEE
                     EGLAGLVTLLRREIGASKATVLVLDGLTAARDQAASNTAFKMFVQELRTQAALHGCTV
                     FLLTTASGQVLAPEYTMVDGIVELMDHRFGSRSQRSLYVNKLRGSDYLPGRHPFRITP
                     EGLVVNPRIEAEFRVSTRLDKAWPGKLSIGVKGVDVMLGGGVPEASVTGLMGPSGIGK
                     TSMGLHFLSGSSSNEPGLCFGFYETPPRSLQKAARFGLDLDAAMRRGDLEILWQAQGE
                     NSQDALAHGLLEAVGRRGVRRLFLDGLGGFIEASVEPDRISRFFSVLANELRARGVTT
                     LYTMETQNILDFGIRMPVTGMSSIVENLIALRYVESGSRSRRLLSVVKVRDSGFDPAV
                     REFVINDGSGVFLAGAFQRLEELWSGSAPERAKEGSSSTPEDQ"
     gene            2315..2695
                     /locus_tag="VGC82_14215"
     CDS             2315..2695
                     /locus_tag="VGC82_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0424473.1"
                     /translation="MTTILVVDDEALIAMALQGALEDEGYQVVTAANGRQGLERLAAL
                     PRPELVLLDMMMPVMSGPAMLQAMQADVALGAIPVIVLSSLPREAVQARTTGVAAILR
                     KPYTVNEILGVIARVLREAGGNAS"
     gene            complement(2891..3118)
                     /locus_tag="VGC82_14220"
     CDS             complement(2891..3118)
                     /locus_tag="VGC82_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424474.1"
                     /translation="MALQLGATREAFIAAGTPADKASAAAEELAGYDNRLAGIETRIA
                     VLVAMVAGLYALLAPGVWMLVRIASKTGALS"
     gene            complement(3304..3378)
                     /locus_tag="VGC82_14225"
     tRNA            complement(3304..3378)
                     /locus_tag="VGC82_14225"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3344..3346),aa:Val,seq:cac)
     gene            3651..4454
                     /gene="dapF"
                     /locus_tag="VGC82_14230"
     CDS             3651..4454
                     /gene="dapF"
                     /locus_tag="VGC82_14230"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY0424475.1"
                     /translation="MTTGFVKMHGCGNDFVVLDERVRPLDLTPVRAAAIADRHTGIGC
                     DQLIVLSPTANADLSMRIYNPDGSEAGACGNATRCVADLLMRQTGRAAVTIRTIAGDL
                     PAVRRPDGLIQVDMGPAQLDWSDIPLARPMDTLRLQLPGHPAAASMGNPHATFFVADL
                     DAEPISETGPRFEWDPLFPQRANIGFAQVLDPQTIRLRVWERGAGLTLACGSGACAAL
                     VNAHRRGLTHRRAMVIVDGGVLEIEWRDDGNVLMTGPVATAFTGEIDLS"
     gene            4451..5686
                     /gene="mtaB"
                     /locus_tag="VGC82_14235"
     CDS             4451..5686
                     /gene="mtaB"
                     /locus_tag="VGC82_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEY0424476.1"
                     /translation="MTVDILTFGCRLNAYESEVMRVLSAAGDLEATVVVNTCAVTAEA
                     ERQARQAIRRVARERPEARIVVTGCAAQIDPASWAALPNVHRVLGNDDKMRPENWLPG
                     AGSAVSDIMSARETAAHLVTEFAGRARAFVQVQQGCDHRCTFCVIPFGRGRNRSVPIG
                     AVADQVRTLVAGGYQEVVLTGVDIASYGPDLPGSPSLGQMVRRLLLAVPELPRLRLSS
                     IDPAAIDDDLWRLIAEEPRLMPHLHLSLQAGSDMILKRMRRRHSRGDAMEVVRRARDL
                     RPGIAIGADLIAGFPTETETLFDQTLSFVAEAALPYLHVFPYSERAGTPAARMPPVPK
                     PVRKQRAAALRDAGRAEAHRFFTAKIGDMVSILTEADRSGHTEHFAPVRLALPCAPGQ
                     LVTARVTGATNDTLLAEAA"
     gene            5686..>6169
                     /locus_tag="VGC82_14240"
     CDS             5686..>6169
                     /locus_tag="VGC82_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEY0424477.1"
                     /translation="MALGGFFTRLKEGLSRSTQKLSGGITAVFKKRRLDDEALEELEE
                     LLISADLGTGAARKVIAAFRRSRFGKEVTDEEIKQALAEEISAILEPVAKPLELNPAL
                     KPHVVLVVGVNGTGKTTTIGKLAQTYREQGKRAVMAAGDTFRAAAVEQLQIWGERTGT
                     P"
BASE COUNT         1087 a         2018 c         1970 g         1094 t
ORIGIN      
        1 cgctctacct tctgcaccga actttctttt aaggaccaca tgacaaaggc cattccggct
       61 ggcgacagcc acgatgcgtc ggaaaaggac cgggagatcg aagaactgcg cgaggcagtc
      121 cgggaactgc gtgaagcggt ccgggcgcgc gacgagttcg tcgcgatcgc ggcgcatgag
      181 ctgcgcaacc cgatgacgcc catcctcatg caggttgata tgctgatgaa tgcggcgaaa
      241 caacccggcc gctgccgccc ggagatcatc atacctcgcc tcgaacgtct cgagttcgcg
      301 gtcggtgagt tcatacgccg ttcaacgact ttacttgatg tatcgcgcat cgcagctgga
      361 aatcttcaga tcgagctttc caaattcgat ctctcgcagg tggtgcggga ggtggtggcc
      421 cgtgccgaac cgagcgcccg catgacgcga tcccacgtcg aggcggacct gcaggagcaa
      481 gtggtcggaa cgtgggaccg gctcgcgatc gaacaggtcg cggagaatct gttgtcgaat
      541 gccctcaagt tcggcgccgg caaaccggtt accgtgacgg tacgccggga cggccggacg
      601 gtccagctcg ttgtccgcga ccagggcatc ggcatctccg aggaagcaag cgctcgaatt
      661 ttccagcgat tcgaacgtgc cgtcggccac agcgagcatg gcggcttcgg cattggcctc
      721 tggctggcca accagctcgt cgtggccatg ggcggcacga tcgacgtcca aagtcgtccg
      781 ggcgaaggag cgacttttct ggtgaatttt gcccatgcag ccgcaagagt aatccaatga
      841 atgagattcg aacgatcgag cgggtgccga gcggggtgcc cggcctggac accatcctga
      901 acggcggctt taccaaatcc ggtatccata ttctggaagg accaccgggg gccggcaaga
      961 cgacattggc caaccagatc tgttattccc atgcagcggc gggtggcgtc gccctgtatg
     1021 tcacgctcct ggccgaaacc catacccagt tgctgctgaa ccttgccacg atgcgcttct
     1081 tcgacctgtc gctcctgccg gagcggatct cctacatcag cggctttggc gccatggagg
     1141 aggagggcct ggcggggttg gtcacgctgt tgcggcgcga gatcggggca agcaaggcga
     1201 ccgtgcttgt tctggacggt ctgaccgccg cgcgggacca ggcagcgtca aacaccgcgt
     1261 tcaagatgtt cgtgcaagaa ttacgcaccc aggcggcact gcacggctgc acggttttcc
     1321 tgctgaccac cgccagcggg caggtccttg ctcctgaata cactatggtg gacggcatcg
     1381 tcgagttgat ggaccatcgc ttcgggtctc gttcccaacg cagcctttac gtcaacaagc
     1441 tgcgtggcag cgattacctt cccggccggc accccttccg catcacgccg gaaggcttgg
     1501 tggtcaatcc gcggatcgag gcggaattcc gggtgagcac ccgcctggac aaggcttggc
     1561 ctggcaaatt atccatcggc gtcaagggtg tcgatgtgat gcttggaggc ggcgtgcccg
     1621 aagcatcggt cactggcctg atgggtccgt ccggcatcgg caagacgtcg atgggcctgc
     1681 atttcctgtc cggatcaagt tccaatgagc cgggtctgtg cttcggtttc tacgaaaccc
     1741 cgccccgctc gctgcaaaag gcggctcgct tcgggctcga tctcgatgca gcgatgcgcc
     1801 gcggggatct cgagatacta tggcaggcgc agggtgaaaa ttcgcaggac gcgctggcgc
     1861 acggcctgct ggaggcggtc ggccggcgcg gcgtcaggcg gctgttcctc gacgggcttg
     1921 gtggcttcat agaggcttcc gtcgagcccg accgcatcag ccgctttttc tccgtgctgg
     1981 ccaacgagct gcgggcgcgc ggcgtgacca cgctctacac catggaaacg caaaacatat
     2041 tggacttcgg tatccgaatg ccggttaccg ggatgtcatc catcgtggag aacctgatcg
     2101 ccctgcgcta cgtggaaagc ggctcgcggt cccggcggct actgtccgtg gtgaaggtcc
     2161 gcgacagcgg gttcgacccg gccgttcgcg agttcgtcat caacgacggc agcggcgttt
     2221 tccttgcggg cgcgttccag cgccttgagg aactctggag cggatccgca ccggagcgtg
     2281 ccaaggaagg gtcttcctcc acgccggagg accaatgacg accatcctgg ttgtggatga
     2341 cgaggcgctg atcgccatgg ctcttcaggg tgcgctggag gacgaaggct atcaggtcgt
     2401 aaccgcggcc aacggcaggc agggactcga gcggctggct gcgcttccgc gcccggagct
     2461 cgtgctgctg gatatgatga tgccggtcat gagtggtccc gcgatgctgc aggccatgca
     2521 ggctgacgtg gccctgggtg ctattccggt catcgtcctg agttccctgc ccagggaggc
     2581 ggtccaggcg cgcaccaccg gcgtcgccgc gatcctgcgc aagccctata ccgttaatga
     2641 aattctcggc gtgatcgcac gggtacttcg ggaagcaggc ggaaatgctt catagccgca
     2701 cgcgcagcag gaggtccgct tgtttggttt ccgcagttga ttgggtacga cggggattag
     2761 gcccacgtga aggcggctga cccgtatgcc tccgtcgact gggaggcatc ttcgcgcttt
     2821 cccgactgtt agctggggat gctcgatgcg gcggacaggg atatttggcg taggctgccc
     2881 cgacagcccc tcaggaaagc gcaccggttt tggatgcgat ccgcacgagc atccagaccc
     2941 ccggagcaag caacgcgtat agcccggcca ccatggccac cagcaccgcg atccgggtct
     3001 caataccggc caggcgattg tcgtacccag ccagctcctc ggccgcagcg gatgccttat
     3061 ccgccggcgt gcccgccgcg atgaacgctt ctctcgtggc ccctagctgc aacgccatgt
     3121 gacggcttcc atgtgtctgc gaacgtacga acgaggctga cgacaagtca acaaatttgt
     3181 atagcgccgg cacggggagc acgtggtccg gtgaaaggcg gcgaccacga gcgacgttgg
     3241 gcaaggacca ctgcgcatac gtcagttttg cacgagcggc gtagcgattg atttcaaagc
     3301 ggatggtggg cgcgacaggg attgaacctg tgacccccgc cgtgtgaagg cgatgctcta
     3361 ccgctgagct acgcgcccat ccgacgcagc gaaggggcgc ttggtaactg gtcggcgccc
     3421 gtactgcaag tccgaaaccc cgggcgaata aaggagccct cgtccggacc cccgaagttc
     3481 ggagcggtgg cacggggtcg gaatcgaggc atcggcatcc tgcgcccgaa tcccagccgt
     3541 ctgccgcgtg ccccaccgcc tctggtcatc aaccgcctga atcgcacaat cccaaccgac
     3601 cgagcacgcc cattctggcg caatcccgcg cgcttctcta tatccgcgcg atgaccaccg
     3661 ggttcgtcaa gatgcatggc tgcggaaacg acttcgtcgt gctcgacgag cgcgttcgtc
     3721 cgctcgacct gacccccgtt cgggccgcgg cgatcgccga ccggcacacc ggcatcggct
     3781 gcgaccagtt gatcgtcctg tcgcccaccg ccaacgccga cttatccatg cgcatctaca
     3841 acccggatgg atccgaggcc ggcgcctgcg gcaacgccac ccgctgcgtc gccgatcttc
     3901 tgatgcggca aaccgggcgc gccgccgtca ccatccgcac catcgcgggc gacctgccgg
     3961 cggttcgccg tcccgatgga ttgatccagg tcgatatggg accagcacag ctggactggt
     4021 ccgatatccc gctcgcgcga ccgatggaca cgctgcgtct gcagcttccc ggccacccgg
     4081 ccgccgcgag catgggcaat ccccacgcca cattcttcgt cgccgacctc gacgccgaac
     4141 cgataagcga aaccggtccc aggtttgaat gggaccccct gttcccgcag cgggccaata
     4201 tcgggtttgc ccaggtgctc gacccccaga ccatccgcct gcgcgtctgg gaacgcggtg
     4261 ccggcctgac tctggcctgt ggctccggtg cctgcgccgc gctggtgaac gcgcaccggc
     4321 gcggcctgac gcaccgccgc gcgatggtca tcgtcgatgg tggcgtgctg gagatcgagt
     4381 ggcgcgacga cggcaatgtg ctgatgaccg gccctgtcgc caccgccttc accggcgaga
     4441 tcgatctgtc atgaccgtcg atatcctcac gtttggttgc cgcctcaacg cctacgagag
     4501 cgaggtcatg cgcgtgctgt cagccgccgg cgatcttgaa gccaccgttg tcgtcaatac
     4561 ctgcgcggtg acggcggaag ccgaacgcca ggcacgccag gccatccgcc gtgtcgcccg
     4621 ggagcgtccc gaagcgcgca ttgtcgttac cggctgcgcc gcccagatcg atccggcctc
     4681 ctgggccgct ctaccaaacg tgcatcgcgt gctcggcaac gacgacaaaa tgcgccccga
     4741 aaactggctg ccgggtgccg gatcggcggt ctccgacatc atgtcggcgc gcgaaaccgc
     4801 cgcccatctg gtcaccgagt tcgccggccg ggcgcgcgca ttcgtgcagg tgcagcaggg
     4861 ctgtgaccac cgctgcacct tctgcgtcat cccgttcggc cgcggccgca accgttccgt
     4921 ccctattggt gctgtcgccg atcaagtccg caccctggtc gcgggcggct atcaagaggt
     4981 cgtgctgacc ggtgtggata tcgcctccta tggccccgat ctgccgggat cgccttcgct
     5041 gggccagatg gtccgccgcc tgctgctggc ggtgccggaa cttccgcggc tacggctgtc
     5101 ctccatcgac cctgccgcga tcgacgacga tctgtggcgc ctgatcgccg aagaacccag
     5161 gctgatgcca catctgcatc tgtcgctgca ggccggcagc gacatgatcc tgaagcgcat
     5221 gcgccgccgg cactcgcgcg gcgatgcgat ggaagtggtc cgacgagccc gtgacctgcg
     5281 tcccggcatc gccatcggcg ccgacctgat cgccggattt ccgaccgaaa ccgagacctt
     5341 gttcgaccag acgctgagct tcgtggccga ggccgccctg ccctatctgc atgtgttccc
     5401 ttacagcgaa cgcgccggga ctccggctgc ccgcatgccc ccggtgccca agcccgtgcg
     5461 aaagcagcgt gccgccgctt tgcgcgacgc cggccgggcc gaggcacatc gtttcttcac
     5521 cgccaagatc ggcgacatgg tctcaatcct gaccgaagcc gaccgatccg gtcataccga
     5581 gcattttgcc ccggtacgcc tggcgctgcc atgcgcaccc ggccaattgg tcaccgcccg
     5641 ggttaccggt gcgacgaacg acaccttgtt ggcggaggct gcctgatggc gctcggtggt
     5701 ttcttcacgc gcctgaagga aggcttgtcc cgcagcacac aaaagctgtc cgggggcatc
     5761 acagcggtgt tcaagaaacg ccgcctggac gacgaggcgc tggaggagct ggaggaactg
     5821 ctgatctcgg ccgatctcgg caccggggcg gcgcgaaaag tgatcgcagc cttccgccgc
     5881 agccgttttg gcaaagaagt caccgacgag gaaatcaagc aggcactcgc cgaggaaatc
     5941 agcgcgatcc tggaacccgt cgcgaaaccg ctggaactga atccggcact gaagccgcac
     6001 gtcgtgctcg tggtcggagt gaacggcacc ggcaagacca ccacgatcgg caagctggcg
     6061 cagacttaca gggagcaagg caagcgcgcg gtcatggccg ccggcgatac gttccgggcg
     6121 gcggcggtgg agcagctgca gatctggggt gagcgaaccg gcactccgg
//
LOCUS       DASWQN010000843         2772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1929246, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000843 DASWQN010000000
VERSION     DASWQN010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929246"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..620)
                     /locus_tag="VGC82_14245"
     CDS             complement(96..620)
                     /locus_tag="VGC82_14245"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY0424478.1"
                     /translation="MSLVLLQLVGLFAGLSMLAFGGGSGAIPDMQRAAVEQHHWLTAR
                     EFLDMFAISRAAPGPGSLIVALIGLKAAGLAGAVLSFAAMFAPSCLAVHIVARYWQKA
                     ARSAWRAVVERALAPIAVGLTFASGLSLMRGTEHGWLPWGVTIASTALFSLTEINPIL
                     LLTCGAAVVLLVGR"
     gene            complement(667..1323)
                     /locus_tag="VGC82_14250"
     CDS             complement(667..1323)
                     /locus_tag="VGC82_14250"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY0424479.1"
                     /translation="METRPASASPSQGPPFPGLPFPDAPSPGAPLLGPPSLGPPSLAS
                     LFTSFVSIGMMSFGGGLAAWTRREVVQRKAWLDDKQFLSGYALCQLVPGATNVNLAVF
                     IGTQLRGPAGALACFFGLTTLPLVIVLILGTWYLHSQGNTSGAWISIALGGIGAVAIG
                     LNLGTGVRLARRNIAGPIPIAITAAVGLSIGVFGFSLLRVLLVVMPVSLLAAFLTRPK
                     "
     gene            1380..2387
                     /locus_tag="VGC82_14255"
                     /pseudo
     CDS             1380..2387
                     /locus_tag="VGC82_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            2422..2498
                     /locus_tag="VGC82_14260"
     tRNA            2422..2498
                     /locus_tag="VGC82_14260"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2456..2458,aa:Arg,seq:cct)
     gene            complement(2511..>2772)
                     /locus_tag="VGC82_14265"
     CDS             complement(2511..>2772)
                     /locus_tag="VGC82_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424480.1"
                     /translation="FLRGLGGTGWFGWYRDEETERLAEAWLQAETQEDRVAITDLIQA
                     RAFETVPAIPLGQFQIRTAHRKNLTGIIEATGAFPWNVRRVG"
BASE COUNT          498 a          805 c         1022 g          447 t
ORIGIN      
        1 ggatgtcctt gcggttatag gtgacgatcc cgataccaac cgaaaaaggc attgttcacc
       61 tttggcaatc tgcggttgtc ctctacggcg gcggcctagc gcccaacaag caaaaccacc
      121 gcggctccgc atgtcagcag caggatcggg ttgatctccg tcaggctgaa cagggccgta
      181 ctggcgatcg tgacacccca gggtaaccaa ccatgttcag tgccgcgcat cagcgacaag
      241 ccgctggcaa aagtcaggcc gaccgcgata ggggccaatg cacgctccac taccgctcgc
      301 caggcggaac gcgcggcctt ctgccagtag cgggcgacga tgtgcacagc gaggcaggac
      361 ggcgcgaaca tcgcggcaaa cgacagcacc gcgccggcca ggcccgcggc tttcaggccg
      421 atcagtgcca cgatcagcga ccccggcccg ggtgcggcgc gggagatcgc gaacatgtcg
      481 agaaactccc gagcggtgag ccagtggtgc tgctcgacgg cggcgcgctg catgtcgggg
      541 atcgcgccgg agccaccgcc gaaggccagc atcgacaggc cggcgaacag gccgacgagc
      601 tggagcagga ccagactcat ggagaatagg ggctcatgtt gagtgggggc tcatgttgga
      661 tggggttcat tttgggcggg tcaggaaggc ggcgagcagg ctgaccggca tgaccaccag
      721 caaaacccgc agcagggaga agccgaaaac gccgatggac aggccgacgg cggcggtgat
      781 ggcgatgggg atcgggccgg cgatgttgcg ccgcgcgagg cggacgccgg tgcccaggtt
      841 cagcccgatc gcgaccgcgc cgatgccgcc gagggcgatg ctgatccagg cgcctgacgt
      901 gttgccctgg ctgtgcaggt accaggtgcc gaggatcagc acgatgacca gcggcagcgt
      961 agtcaggcca aagaaacagg caagcgcccc ggccgggccg cgcaactggg tgccgatgaa
     1021 taccgcgagg ttgacattgg tggcccctgg cacgagttgg cacagcgcat atccggacag
     1081 gaattgtttg tcgtcgagcc aggcttttcg ttgcacgacc tctcgccttg tccaggcggc
     1141 gaggccgcca ccgaagctca tcatcccgat gctgacgaag ctggtgaaca gcgacgccag
     1201 ggatggcgga cccagggatg gtggacccag gagtggcgcg cctggggatg gcgcatctgg
     1261 gaatggtagg cccgggaacg gcgggccttg ggatggcgag gcggaggctg gtcttgtctc
     1321 catcggtgca ttgaagcctg tccggtgtag ggtggacaga ggcaagcagg aggctacgga
     1381 tggaataccg gtcgttgggt cgcagcgggc tgaaagtgtc gcctttatgc ctgggcacga
     1441 tgatgttcgg aggtgctacc gaggatgcga cggcggcgcg gatcgtggcg cgggcgaagg
     1501 agcagggcgt caatttcatc gactcggccg acggctacaa cggcggccgg tccgaggaag
     1561 tggtaggccg agcgatccgg gaccatcggt cgtggtgggt gctggcgacg aaatgcgcca
     1621 acccgacggg gcaggggccc aatgctcggg gattgtcgcg gcggcatgtg cagcatgcgg
     1681 tggaagccag cctgcggcgg ctgggcgtcg aagagatcga cgtgctgtac ttgcacaagg
     1741 aagatcactc cacgcccctg gcggagacgg tgcatgcgct ggcggacctg atccgcgccg
     1801 gaaaaatccg ctacttcggg gtttcgaact accggagctg gcgggtggca gagatttgcc
     1861 ggctttgcga cgaggccgga atcgatcggc ccgtggccag tcaaccgctg tacaatgcgc
     1921 tgaaccgcga agtggagacg gagcatctgc cggcttgcgg gtattttggg ctgggcgtgg
     1981 tgccgtacag cccgctggcg cgtggcgtgc tgacggggaa ataggtgccg gacgtgccgc
     2041 ctccggccgg gacgcgcgcg ggccggcagg atcggcggat gctggagtcc gaatggcgcc
     2101 ccgaaagcct gcatatcgcg cgcgaaatcg ccgaccatgc tcgggggaag ggggtctcgg
     2161 cggggcagtt cgcgtttgcc tgggtgttga acaatcgctt cgtgacggcg gcgatcggcg
     2221 gccccaggac cgaagagcag tgggaggatt atgtcgcggc gctggcgtgc cggctggatg
     2281 cggcggatga ggctttggtg gatcggctgg tgtcgccggg acatccgtcc acgcccgggt
     2341 ataatgatcc ggcttatccg atcgaagggc gggtggcccg cggttgatgc ggtcggggcg
     2401 cctctgtata agagtggccg ggtccccgta gctcagccgg atagagcact agattcctaa
     2461 tctaggggcc gtgcgttcga atcgcgccgg ggacgccatc tcgatcgctt ctatcccacc
     2521 cgccgaacgt tccacggaaa tgcgcccgtc gcctcgatta tcccggtgag gttcttgcgg
     2581 tgagcggtgc ggatctggaa ctggccgagt ggtatggcgg gcacggtctc gaaggcgcgc
     2641 gcttggatca gatcggtgat tgcgacgcgg tcttcctgcg tttccgcctg cagccaggcc
     2701 tcggccagcc gctcggtctc ctcgtcgcgg taccagccga accagccggt gccaccgagg
     2761 ccccgtagga at
//
LOCUS       DASWQN010000844         5058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1136552, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000844 DASWQN010000000
VERSION     DASWQN010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136552"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VGC82_14270"
     CDS             <1..219
                     /locus_tag="VGC82_14270"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF021883.4"
                     /note="Catalyzes the cycloisomerization of
                     cis,cis-muconate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEY0424481.1"
                     /translation="LAPVIGRAEAHHVVKHACDIALAEKISLADALARDPAVSSRLDR
                     AAIERLIDPANYLGSTQGFIDRVVTAAR"
     gene            complement(238..870)
                     /locus_tag="VGC82_14275"
     CDS             complement(238..870)
                     /locus_tag="VGC82_14275"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEY0424482.1"
                     /translation="MDLKGYLARCPLIAILRGIRPDEAVAVTTSLELRGIAIVEVPLN
                     SPDPMTSIAALARAFGDRLLIGAGTVMTEAQVGEIAAAGGKLIVTPHADAAVVRAAKR
                     HGLLAVPGIFTPAEAFAMLGAGADALKLFPAEGASPAMLRSMRAVLPFGTMVLPVGGI
                     DASNMSEWMAAGAVGFGIGSAIYKQGDSAETVSAKALALVAALVPAGAPA"
     gene            complement(861..1775)
                     /locus_tag="VGC82_14280"
     CDS             complement(861..1775)
                     /locus_tag="VGC82_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEY0424483.1"
                     /translation="MIGVDWGTTSFRAFRIGRDGTIRDRRSAAKGIMNVPDGRFGDTL
                     REEVGPWLAAGESQVLLSGMIGSRQGWKEASYLPCPAGAAELGAALVDVGFDWARVKL
                     IPGLSGVDDSGVAEVMRGEETQVLGVPPLLRDGGIACLPGTHSKWVRVEAGRIVSFTT
                     HMTGEIFGALRGHTILGRMMLDGPADGLPFDAGLARAADPGGLLHHVFGVRALALAGR
                     LSESDAPAYLSGILIGHEVRAALAGTPSTGAPSTGAAGTVVQVIGAPELTALYAKAIT
                     ACGGFAERHDGEAAARGLALIAEHAEWT"
     gene            1960..2721
                     /gene="pgeF"
                     /locus_tag="VGC82_14285"
     CDS             1960..2721
                     /gene="pgeF"
                     /locus_tag="VGC82_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY0424484.1"
                     /translation="MAEALTTDLIKTPHGFFTRRGGVSSGSYASLNCSLSSPDERSAV
                     LENRARVARSLGTDPDRLLGLTQVHGTRVVTATEAWAAGHGEKADAMVTDRPDLALGI
                     VTADCAPVLFAGSGGAIVGAAHAGWRGAVAGVLEATIAAMVALGASAGRITAAIGPCI
                     AQQSYEVGADLRDTVLLRSAADSAFFAPGKSSDRWQFDLPGYCAARLRVVGVGHVVVT
                     GHDTLLEEDRFFSHRRRTLAKAGPIGHQISVIRAA"
     gene            2813..3730
                     /locus_tag="VGC82_14290"
     CDS             2813..3730
                     /locus_tag="VGC82_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424485.1"
                     /translation="MALLLSLVVLPCLGLAACGDLPEPFLGNPGANARRLAQPLTSLL
                     AVPPGTNTLLPDAANQELAKQIAVALQATEVPAMVRTPEKTDWRLITTAQRNGDYVVP
                     LFSVLDPQGKEQGTAQGEKVPLDVWSNADPALLSQLAAEAAPRIGAVLTSIRIAHDKT
                     DPGSLYNRPAKVLVADVTGAPGDGNLSLTQQMRSRLAVLGPVVLTTPAGADFVLQGEV
                     KVVPLPKRQERVEIQWIIKTASGDERGRVVQLNEIPAGTLSRYWGDVAVVVASEASSG
                     VNDVILRQAGHDPNETRQSAKQEAHQSAK"
     gene            3893..4348
                     /locus_tag="VGC82_14295"
     CDS             3893..4348
                     /locus_tag="VGC82_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424486.1"
                     /translation="MAFKGKPPGSGAGDISKRTEELRPVLLLTAKEASEARGMRRSGL
                     SAEDIAIAMGKPVDEIEKVLVQMRMPRPETTRGTLNVTLAAHRLIMHERQGDEPIWTT
                     MDRLLDELLRLRQAQAAQSAAPPPSQRRASRRLDADASLPLLAGIGFPE"
     gene            4423..4908
                     /locus_tag="VGC82_14300"
     CDS             4423..4908
                     /locus_tag="VGC82_14300"
                     /inference="COORDINATES: protein motif:HMM:NF045247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424487.1"
                     /translation="MSRPWIPRRVLVTPSALGWPSGVAMTERAAALGSNIVRLDSDRL
                     TGLPDTYRDAKTTMAIVAASPSRRRLQPIPPSADWRFDLAEGCPAHCSYCYLAGSLKG
                     PPIIRAYANLPEILAELPSAAGHGRVTSTSLGRTAEGTTFECSCYTDSLAIEHLTGHL
                     E"
BASE COUNT          851 a         1672 c         1687 g          848 t
ORIGIN      
        1 cttgccccgg tgatcggtcg tgcggaggcg catcatgtcg tgaagcacgc ctgcgatatc
       61 gcgttggccg agaagatttc cctcgctgac gcgctggcac gcgatccggc ggtttcgtcc
      121 cggctggacc gcgcggcgat cgaacggctg atcgacccgg ccaactacct tggcagcacc
      181 caaggcttca tcgaccgcgt cgtcacggcg gcccggtagg cgtccggcac ggaacgatca
      241 ggccggagcc cctgccggaa ccagggcggc gaccagggca agcgccttgg cgctgacggt
      301 ttcggccgag tcgccttgtt tgtagatcgc cgatccgatg ccgaacccga ccgcgcccgc
      361 cgccatccac tcgctcatgt tggaggcatc gatgccgccg accgggagca ccatcgttcc
      421 gaacggcagc accgcccgca tggaccgcag catcgccggg ctggcaccct cggcgggaaa
      481 cagcttcagg gcgtcggcac cggcgccgag catggcgaag gcctccgccg gagtgaaaat
      541 tccgggcact gcgagcaggc cgtggcgctt ggcggcccgc accacggcgg cgtcggcgtg
      601 cggtgtcacg attagcttgc ctccggcggc ggcgatctcg ccgacctgag cctcggtcat
      661 gacggttccg gcgccgatca gcagccggtc gccgaacgca cgggccagtg ccgcgatgct
      721 ggtcattggg tccggtgagt tgagcggcac ctcgacgatg gcgatgccgc gcagttcaag
      781 ggacgtggtg accgcaaccg cttcatccgg ccgaatgccc cgcaggatgg cgatgagcgg
      841 gcagcgcgcc agatatccct tcaagtccat tccgcgtgct ccgcgatcag tgcgaggccg
      901 cgcgcggcgg cttcgccgtc atggcgttcg gcgaagccgc cgcatgcggt gattgctttg
      961 gcgtaaaggg cggtcagttc cggcgcgccg atcacctgca ccaccgtgcc cgctgctccg
     1021 gtcgaaggtg cgccggtcga aggtgttccg gccagcgccg cccgcacttc atggccgatc
     1081 aggatgccgg acaagtaggc cggcgcgtcc gactcggaca ggcggcccgc cagcgctaag
     1141 gcccgcacgc cgaacacatg gtgcagcagg ccgcccggat cggccgcccg agcgagaccg
     1201 gcgtcgaacg gaaggccgtc ggcgggacca tcgagcatca tccggcccag aatcgtgtgg
     1261 ccgcgcaggg cgccaaatat ctcgcccgtc atgtgcgtgg tgaagctgac gatccggccg
     1321 gcttccaccc gcacccactt cgaatgcgtt cccggcaggc aggcgatgcc gccgtcacgc
     1381 agcaacggag gcacgccgag gacctgggtt tcttcgccgc gcatgacctc ggccacaccg
     1441 ctgtcatcga cgcccgacag gccggggatc agtttgaccc gtgcccagtc gaatccgaca
     1501 tcgaccagcg cggcacccag ttcggccgct ccggcagggc agggcaggta ggaggcttct
     1561 ttccagccct gacggctgcc gatcatgccg gatagcagga cctggctctc cccggcggcc
     1621 agccacgggc cgacttcctc gcgcaacgtg tcgccgaagc gtccgtccgg aacgttcata
     1681 atgcctttcg cagccgaacg ccggtcgcgg atggtgccgt cgcgcccgat gcggaatgcg
     1741 cggaaactgg ttgtgcccca atcgaccccg atcatcgcgt tgtccttccc agcttgtctt
     1801 gcccagcttt atccctgccc ggcgttctct ggcccccggc gttatctggc ctctggagtt
     1861 gcctggcctg ggggctgtca cacgccagcg taacacagtt gaccggtggc ggcacattgc
     1921 gcacatcgaa gctatcgacg ttcccggagt cccaccacga tggcggaagc cctgaccacg
     1981 gacctgatca agactccgca tggattcttc acgcgtcgcg gcggcgtgtc cagcggttct
     2041 tacgccagcc tcaattgcag cctgtcgagt ccggacgaac ggagcgcggt tctggaaaac
     2101 cgcgcccgcg tggcgcgatc cctcggtacc gatcctgacc ggctgctggg gctgacccag
     2161 gttcatggca caagggtggt caccgccacc gaagcctggg ctgccggcca tggcgagaaa
     2221 gccgatgcca tggtcaccga ccggccggac ctggcgctcg gtatcgtgac ggcagactgt
     2281 gcgccggtgc tgttcgcggg ttccggtggc gcaatcgtcg gtgccgccca cgccggctgg
     2341 cgcggcgccg tagccggtgt gctggaggcg acgattgcgg caatggtcgc actgggcgca
     2401 tcggcgggtc ggatcaccgc cgccatcggc ccctgcatcg cgcagcaatc gtatgaggtt
     2461 ggggccgacc ttcgggacac cgttctgctt cgatcggccg ccgactcggc gttcttcgcc
     2521 ccgggaaagt cttccgaccg ctggcagttc gatctgcccg gctactgtgc cgcccgactc
     2581 cgcgtcgtcg gggtgggtca cgtcgtcgtg accggacacg acacattgct ggaagaggat
     2641 cgctttttca gccaccgcag gcgcacgctc gccaaggcgg gtccgatcgg ccaccaaatc
     2701 tcggtcatca gggcggcctg agcggaacaa tgagacggtg ctgcgggaaa gccccttccg
     2761 aaggcttgcg gcatctgctt caagcggcgg aatgatcgct cgtcttggcc gtatggctct
     2821 gctcctctcg ctcgttgttc ttccctgcct cggtttggcc gcttgcggcg accttccgga
     2881 gccgttcctg gggaatccgg gggcaaacgc acgccgattg gcgcagccgc tgacgtcgtt
     2941 gctggcggtg ccgccgggga ccaatacgct gctgcccgac gcggcgaacc aggaactagc
     3001 gaagcagatc gccgtggcgc tgcaggcaac cgaggtaccg gccatggtgc gcacgccgga
     3061 gaaaaccgat tggcgcctga tcaccacggc gcaacgcaac ggcgattacg tcgtgccgct
     3121 gttcagcgtg ttggatcctc agggcaagga gcagggcacc gcgcagggtg agaaggtgcc
     3181 gctggacgtc tggagcaacg ccgatccggc tttgttgagc caactcgcgg ctgaggcggc
     3241 gccgcgcatc ggcgctgtgc tcacgagcat ccggatcgcg cacgacaaga ccgaccccgg
     3301 gagtttgtac aaccggccgg caaaggttct ggtggccgac gtcaccgggg cgcccgggga
     3361 cggcaatttg tcgctgacgc agcagatgcg ctccaggctg gccgtgcttg gacccgtggt
     3421 cctgacaaca ccggcgggcg cggacttcgt cctgcaaggt gaagtcaagg tggtgccgtt
     3481 gcccaagcgc caggagcgag tggaaatcca gtggatcatc aagaccgcgt cgggcgacga
     3541 gcggggacgg gtggtgcagt tgaacgagat tccggccggg acgctgtccc gctactgggg
     3601 cgatgtcgcg gtcgttgtcg ccagcgaggc ttccagtggc gtcaacgacg tgatcctgcg
     3661 gcaggccggc catgacccga acgaaacccg ccagtcggcg aagcaggaag cccaccagtc
     3721 ggcaaagtag gaaacccacc agtcggcgaa gccggaaacc caccagtcgg cgaagtcgtt
     3781 tgctatgtcc ggggtctttc gcggcctcag ttccaagaat atttccgggc tgccggtagc
     3841 ccggacgtga tatacacttc gcacgaagcc gaatgtgcag gagtcggtgc ggatggcctt
     3901 caagggtaag ccccccggca gtggcgccgg cgatatctcg aaacgcactg aagaactgcg
     3961 tcctgtcctg ctgctgactg ccaaggaggc gtcggaagcc cgagggatgc ggcgatcggg
     4021 cctgtccgcc gaggacatcg ctattgcgat gggcaagccg gtggatgaga tcgagaaggt
     4081 cctggtgcag atgcgcatgc cacggccgga gactacccgg ggcacgttga atgtcacctt
     4141 ggccgcacac cgtctgatca tgcacgagcg ccaaggcgac gaaccgatct ggaccacgat
     4201 ggaccggctg ctggatgaat tgttgcggct gaggcaggca caggcggccc aaagcgcagc
     4261 gccgccgccg tcgcagcggc gtgccagccg gcggcttgac gccgatgcgt ccttgccgtt
     4321 gctcgcggga attggtttcc cggagtagcg catcgcattg tcgttccatg gagcggcgcc
     4381 tgaggcactg gcaaatccgc gttctcgccc gacatgccgg tcatgtcgcg cccttggata
     4441 ccccgacgtg tacttgtcac cccatcggcc ctcggttggc cgagcggcgt cgccatgacc
     4501 gagcgggccg cggcgcttgg ttcgaacatc gtgcgtctcg attccgaccg tctgactggt
     4561 ttgcccgata cataccgcga cgcgaaaacc actatggcga tcgtcgccgc gagcccgagc
     4621 cgtcgccgcc tgcaaccgat cccgccaagc gccgactggc ggttcgatct tgccgaaggt
     4681 tgtccagccc attgcagcta ttgctacctc gccggttcat taaaggggcc gccgatcatt
     4741 cgggcctatg ccaatctgcc tgaaatcctg gccgaactgc ccagcgctgc cggccatggc
     4801 agggttacgt caacctcgtt gggccggacc gcggaaggga cgacgttcga gtgctcctgc
     4861 tacacagact cactggcgat cgagcacctg accggccatc tagagtagat cccgttcaga
     4921 tagattatct gaacgggtga agatgctcgc taaaacaatg agctagaccg ttttcaggta
     4981 acaggggcaa aaccccgggc atgccgtggt atcaggcggg ctggccgcgg catcggacga
     5041 gttgcggcaa tggcggaa
//
LOCUS       DASWQN010000845         3534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2193285, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000845 DASWQN010000000
VERSION     DASWQN010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2193285"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VGC82_14305"
     CDS             complement(<1..289)
                     /locus_tag="VGC82_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyllide reductase subunit Z"
                     /protein_id="HEY0424488.1"
                     /translation="MLVLDHDRAGGYWGAVYAFTAIRGLRVIIDGPVGCENLPVTAVL
                     HYTDALPPHELPVVVTGLSEEELSRNGTEASMRRAAATQDPDMPAVVVTGSI"
     gene            complement(293..1723)
                     /gene="bchY"
                     /locus_tag="VGC82_14310"
     CDS             complement(293..1723)
                     /gene="bchY"
                     /locus_tag="VGC82_14310"
                     /EC_number="1.3.7.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyllide a reductase subunit Y"
                     /protein_id="HEY0424489.1"
                     /translation="MLEAARVAGKSESLDRLLADYPRGPHDQPQSMCPAFGSLRVGLR
                     MRRTATILCGSACCVYGLTFTSHFYGARRSVGYVPFNSETLVRGQLYEDIRNAVFETA
                     KPADYDAVVVINLCVPTASGVPLDRLPKAIDGVRIIGIDVPGFGVPTHAEAKDVLSGA
                     MLRYARQEAQSGPVARPRALADMPTVTLIGELFPADPVGVGALLAPMGLAVGPQVPSR
                     EWRDLYGALDCAAAAAVHPFYTASIREMQGAGRPVVGSGPVGVEGTAAWLDAIGNATG
                     ISSDAAKARALPAIRSALDANPIDARITVSGYEGSELLVARLLIEAGAEIPYVGTACP
                     RTEWSESDRAWLQARGTHVQYRASLEQDLAAMLEVRPDLAVGTTPLVQKAKALGIPAL
                     YFTNMVSARPLFGPAGAGALAAIVAAQTKGRDRFARMVSFFDKVGNGETAGYGWTNVP
                     KDPPGAKDRYRRAREAKAKASQAVGT"
     gene            complement(1770..2692)
                     /locus_tag="VGC82_14315"
                     /pseudo
     CDS             complement(1770..2692)
                     /locus_tag="VGC82_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyllide a reductase iron protein subunit
                     X"
     gene            complement(2758..>3534)
                     /gene="bchC"
                     /locus_tag="VGC82_14320"
     CDS             complement(2758..>3534)
                     /gene="bchC"
                     /locus_tag="VGC82_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyll synthesis pathway protein BchC"
                     /protein_id="HEY0424490.1"
                     /translation="PFPGMGYPLVPGYESVGRIVAAGAGNETRIGQTVFVPGASCFGE
                     IRGLFGGAASRLVVPASRAMPIDSSLAERGILIALAATAHHAIAGGELPGLIVGHGVV
                     GRLLARLVVVAGGQPVVWEIDPSRRGGATGYEVLHPDDDKRRDYRCICDASGDPGLLD
                     TLIGRLAFGGEIVLAGFYADRVSFAFPMAFMREARLRVAAQWQADDLHAVGRLIGNGR
                     LSLDGLITHRADATQADAAYRTAFGDPACLKMILDWRASA"
BASE COUNT          622 a         1155 c         1174 g          583 t
ORIGIN      
        1 cgatgctgcc cgttacgacc accgccggca tatcaggatc ctgtgtcgca gcggcccgcc
       61 gcatcgaggc ttccgtgccg ttcctggata attcttcttc ggacaggccg gtgacgacaa
      121 cgggcagttc atgcggcggc agggcatcgg tgtagtgcag aacggcggtg acaggcagat
      181 tttcgcagcc gacaggtccg tcgatgatga ctcgcaggcc acgtatcgcg gtaaacgcat
      241 aaactgcgcc ccagtatccg ccagcccgat catgatccaa aacgagcatg cgtcaggttc
      301 ccacggcctg ggatgccttg gccttcgcct cgcgggcgcg gcggtagcgg tctttcgccc
      361 caggcgggtc ctttggaaca ttcgtccagc cgtatccggc ggtttcgccg ttgccgactt
      421 tgtcgaagaa actcaccatc cgcgcgaaac ggtcccggcc cttggtctgt gccgcaacga
      481 tcgcggccag tgcgcccgcg ccggcggggc cgaacaacgg tcgcgcggag accatgttgg
      541 tgaagtagag cgccgggatc ccaagcgcct tggccttttg cacgagcggt gtcgtgccga
      601 ccgcgaggtc gggtcggacc tccagcatcg ccgcaaggtc ctgctccagc gatgctcggt
      661 attgcacatg cgtgccgcgc gcttgcagcc aggcgcggtc ggactcgctc cactcggtcc
      721 tcgggcaagc cgtgccgacg taagggatct cggcgccggc ttcgatcagc agtcgggcga
      781 ccaggagttc ggacccctca tagccggaaa cggtgatccg cgcgtcgatc gggttggcat
      841 ccagcgccga ccggatggcc ggcagcgcac gggctttggc ggcgtccgaa gatattccgg
      901 tggcgttgcc gatcgcgtcc agccaggcgg cggtgccctc gacaccgacg ggaccggagc
      961 cgacgaccgg gcgtccggct ccctgcatct cgcgtatcga tgctgtgtag aacggatgaa
     1021 cggctgccgc cgcggcgcag tccagcgcgc catatagatc gcgccactcg cgcgatggga
     1081 cctgtggccc gaccgccagg cccatcgggg ccagcaaggc gccgacaccc acgggatcgg
     1141 cgggaaaaag ctccccgatc agcgttacgg tcggcatgtc ggccagtgcg cgtggccggg
     1201 cgacaggccc ggactgggct tcctggcgcg cgtagcgcag catcgcaccc gacagcacgt
     1261 ccttggcctc ggcatgggtg ggtacaccga agcccggaac atcgataccg atgatgcgca
     1321 cgccgtcgat ggctttcggc agacggtcca gcggcacgcc ggacgctgtg ggcacgcaca
     1381 ggttgatgac cacgacggcg tcgtaatcgg ccggtttcgc ggtctcgaag acggcgttcc
     1441 tgatgtcctc gtaaagttgc ccgcgcacca gcgtttcgga gttgaacggc acgtagccga
     1501 cagaccgcct ggcgccatag aaatgcgaag taaaggtcag gccataaacg caacacgccg
     1561 aaccgcagag gatggtggcg gtccgacgca tgcgcaggcc gacgcgcaag gacccgaagg
     1621 ccgggcacat cgactggggt tggtcgtgcg gtccgcgtgg atagtccgcc agcaggcggt
     1681 ccagcgactc gctcttgccg gccacccggg cggcctccag catcgtttct cggccaccat
     1741 ggcaatcggg tgcgatcgaa ccgtccacgt caggcgcctt cgtagatgac ttcgagcgac
     1801 ggccgttcca gcaccgtcgc cgcgccatgt cttcaagcgt cgccggatcc agcacgacat
     1861 cccgaccgac cgcatcgccg gcgaacaggc ccagcagcgc gtcgtggctc agtggcgatg
     1921 ggcgcaccgg gggtgcggag gccacctgtt ccgcgagttg ctcgaacacc gccgcccacc
     1981 ggccgccggg cttgccgata atctcgtagc tggcgctttt gcggcgaatg tcgtcgtcgg
     2041 cgggtatcgc ggacagcacc gggatgccga cggcattggc gaaggcctgt gcctcgccgg
     2101 tgccgtcgtc cttgttgacg accatgccgg cgacgccgac attgccgccg agcttgcgaa
     2161 agtaatcgac ggcggagcaa acgttgttgg cgacgtaaag cgactgcagg tcgttcgacg
     2221 caacgacgat gactttctgg cacatgtcgc gggcgatcgg caggccaaaa ccgccgcaga
     2281 ccacgtcgcc gaggaaatcc agcaggacat agtcaaatcc ccattcatgg aaaccgagtt
     2341 tttcgagcaa ctcgaagcca tgaatgatgc cccggccgcc gcagccgcgg ccgacctcgg
     2401 gtccgccgag ttccatagcg aagacgccgt cgcgcttgaa gcagacgtcg ccgatcttga
     2461 ctgcctcgcc ggccagtttc ttcttcgacg acgtctcgat gatggtcggg cagcttcgcc
     2521 cgccaaacag caacgacgtg gtgtcgcttt ttggatcgca gccgatcagc aacacgcgtt
     2581 tgccctgctg tgccatcatg taggacaggt tcgccagggt gaagcttttg ccgatgccgc
     2641 ccttgccgta gatcgcgata acctgtgttt ctttcttgac cggtccggtc atcggggcat
     2701 cgggctcgat cgccgcctct tggcgcagcc gtgtcaggtt cgcatcgaag ggtgcgttca
     2761 tgcgctagct ctccaatcga ggatcatttt caggcaggcg gggtcgccga aggcggtgcg
     2821 gtaggcggca tccgcctgag tagcgtcggc gcggtgggtg atcaggccgt cgagcgagag
     2881 gcggccattg ccgatcagac ggccgacggc gtgcagatcg tcggcctgcc attgtgcagc
     2941 cacgcgcaac cgggcttcgc gcatgaaggc catcgggaag gcgaacgaca cacggtcggc
     3001 gtaaaagccg gcaaggacga tttcgccgcc gaacgcgagg cggccgatca gcgtatccag
     3061 caggccgggg tcgccgctgg cgtcgcagat gcaccggtaa tcacgacgtt tgtcgtcgtc
     3121 gggatgcaac acctcgtatc cggtcgcacc gcctcgccga ctgggatcga tttcccagac
     3181 cacgggttgg ccgccggcca cgacaaccag acgggccaac agcctgccga ccacgccgtg
     3241 gccgacgatc aggccaggca gttcgccgcc ggcaatcgca tgatgggctg tggcggcaag
     3301 ggcaatcagg atgccgcgtt cggccaagct cgaatcgatc ggcatggcgc gggaggctgg
     3361 cacgacgagg cgtgatgccg cgccgccgaa caggccgcgt atttcgccga aacagctggc
     3421 gccgggaacg aagacggtct ggccgatgcg cgtctcgttg cccgcaccgg cagcaacgat
     3481 gcggccaacg gattcatagc cggggaccag cggatacccc atccccggaa atgg
//
LOCUS       DASWQN010000846         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1005261, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000846 DASWQN010000000
VERSION     DASWQN010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005261"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VGC82_14325"
     CDS             complement(<1..1247)
                     /locus_tag="VGC82_14325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY0424491.1"
                     /translation="MRQGRTHVGEALCLAKEGQLLIAVQAGQEQASEASAETVPLPPL
                     RLTVDGSYPHAPACTKDDDKVANMPGETDPHVPHRAMITACAIGATVLQLLDQTIANV
                     ALPYMQGSFSSSFDEITWVLTSYITASAIMTAPVGWLAIRYGRKRLYVGCILGFTITS
                     MLCGAAQSLGQIVMFRVLQGLFGAALVPLSQATLLDIYPQERRGFAMAIWGVGVMLGP
                     IMGPTIGGWLTETYSWRWVFYINLPFGLLAAAGLMIFLPGGGEQGRLRFDWLGFGMLT
                     MGIGAMQLMLDRGQIQDWFTSREVIVEAVLAGLGFYLFLVHALSARQPLIRPALFKDV
                     NFASGVLLMFMVGIFMVSSLALMTPWLQVLSHYPVETAGLIMAPRGFGSLVTTMMTGR
                     LAARIDARLLVGIGLLLLTYSYWL"
BASE COUNT          336 a          505 c          474 g          238 t
ORIGIN      
        1 agccaatagc tgtaagtgag cagcagcaat ccgattccca ccagcagccg cgcgtcgatg
       61 cgcgcggcca ggcgtccggt catcatcgtg gtcacgaggc tgccaaaccc gcgcggcgcc
      121 atgatcagcc ccgcggtttc caccgggtaa tggctcaata cctgaagcca tggcgtcatc
      181 agtgcgaggc tggacaccat gaagatgccg accatgaaca tcagcaggac gccggatgcg
      241 aaattgacgt ccttgaacaa tgccggacgg atcagcggct ggcgcgcgga caacgcgtga
      301 accaggaaaa ggtaaaatcc cagcccggcc aacaccgcct cgacgatgac ctcccgggag
      361 gtgaaccagt cctggatctg gccgcggtcc agcatcagct gcatcgcacc gatccccatc
      421 gtcagcatgc caaagccaag ccaatcgaag cgcaggcgcc cctgctcgcc accgcccggc
      481 aggaaaatca tcaggccggc ggcagcgagc aggccgaacg gcagattgat gtagaacacc
      541 cagcgccaac tgtaggtttc ggtcagccag ccgccgatcg ttggacccat gatcggccct
      601 agcatcacgc cgacacccca aatcgccata gcgaagccgc gccgctcctg ggggtagatg
      661 tcaagcagcg tagcctgcga taacggcacc agggcggcgc cgaacaggcc ctgaagcacg
      721 cgaaacatga cgatctggcc caaagactgg gccgcgccgc agagcatcga ggtgatggtg
      781 aagcccagga tgcaaccgac atagaggcgc ttgcgaccat agcggatcgc cagccagccg
      841 accggcgcgg tcatgatcgc ggacgcggtg atgtaggagg tcaagaccca ggtgatttcg
      901 tcgaagctcg acgaaaaact gccctgcatg tagggcagcg cgacgttggc gatggtctgg
      961 tcgagcagct gcaacacggt ggcgccgatc gcgcacgccg tgatcatggc tcggtggggc
     1021 acgtgggggt cggtttctcc cggcatgttg gcgaccttgt cgtcgtcctt ggtgcaagcc
     1081 ggagcatgcg gataagaacc atccaccgtc aaacgcaacg gtggcaatgg gaccgtctcc
     1141 gcgcttgcct cacttgcttg ttcctgaccc gcctgcacgg caatgagcag ctggccttct
     1201 ttggcgaggc acaaggcctc tccgacatgc gtgcgtccct gcctcatctc gcgtcgctgc
     1261 ggaaaaagaa atgggcggtc tacgccaaac caccctttgc cgagccggag gcggcgcctg
     1321 atcgtgaccg aaccatccgg cgtttgacgc cggcgagaca tcgtaagcat cctgtccgcc
     1381 gctgagcgga caacgggacg acgacaagat cgccaccatg ccgggagaaa cagtccacca
     1441 cgacacgccc catcggggaa tgatcaccgc gtgcgcgatc ggcgccacgg tcttgcagtt
     1501 gctcgaccag accatcgcca acgtcgcgct gccctatatg cagggcagtt tct
//
LOCUS       DASWQN010000847         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1665355, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000847 DASWQN010000000
VERSION     DASWQN010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665355"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1259)
                     /locus_tag="VGC82_14330"
     CDS             complement(42..1259)
                     /locus_tag="VGC82_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424492.1"
                     /translation="MTPALRQIVFINTAHTITHYSLLILPTAVLAMAVPNGPFGESYG
                     PILALATGMFVLYGLLSLPQGWLAQRFGRKVLMTAFFLGTAGGLIAAGLAHSPVMLAV
                     ALAVAGGFAAIYHPIGTTMLVEAAGDKPGRSIGVNGVFGNLGVALAPVVTAFLANAVG
                     WRAAFIAPGLLCAAAGLLWMRTPLAQANIHANRRPFPPIPRHLVRRAVLVLMLTATSS
                     GLVFNAFTLLLPKLLQERLADNAELLPLVGAAAFLVTLCGALTQFSVGRMIDRNTLKK
                     VFLPISLVLAPAMLALSFAQGWLILPLAGAVAASIFGQVTVNETMAARYISPELRARI
                     YSVRFFVGFLGAAAAAPVVSILYERTGSVAAVTMLLAGFSVVTLGCALFFPDRKEELH
                     PELWATALPAAAE"
     gene            complement(1518..2273)
                     /locus_tag="VGC82_14335"
     CDS             complement(1518..2273)
                     /locus_tag="VGC82_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0424493.1"
                     /translation="MQQRRILLVTGAASGIGAAVCRAMARPETAILVHTRRNREGAEA
                     VARNIRDAGGLAAVALGDLAEPAVAEALVATTVEQFGRLDVLISNAGFADRTAFADLT
                     SQALTVSTETIQGAFFRLARAAMPHLHGASHGRVIAVSSFVAHAFRTDLTTFPASAAA
                     KAGLEALVRALAIELGPAGITVNAVVPGFIRKDEGAHRAIGQGVLADQTARIPLGRIG
                     LPEEVAAVVAFLASPAAAYVTGQAIHVDGGLVI"
     gene            2430..>3116
                     /locus_tag="VGC82_14340"
     CDS             2430..>3116
                     /locus_tag="VGC82_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0424494.1"
                     /translation="MRHLSADVLVIGAGGAGMYAAIEAARAGANVLLLDRSLIGRGGA
                     TIMAQMTVAAAVGPPGSDHWRHHLADTLVAGRGLCDPELARLLCEAGVEVIQEMERWK
                     VGWARDGAHLRSVQAPGHDRPRCVYVDFLSTGPAVSKTLRGVVQRTPRIERLGDALVT
                     DLVTRDGVVTGAVVVHIETGKVLTIGANATIIATGGLTRLYRRNSASANMGGDGYALA
                     LRAGAELIDME"
BASE COUNT          563 a          984 c         1070 g          499 t
ORIGIN      
        1 cggtttgcaa atggaggttc ccgaggtgga cgggccgcca gctactcggc agcggccggc
       61 aacgcggtgg cccatagttc cgggtgcagt tcttccttgc ggtcgggaaa gaacaatgcg
      121 cagcccaagg tgacgactga aaacccggcc agcagcatgg tgaccgcggc gacgctgccg
      181 gtgcgctcat agagaatcga gaccaccggt gcggcggcag ccgccccaag gaagccaacg
      241 aagaaacgca ccgagtagat gcgggcgcgt agttcgggag aaatatacct ggcggccatc
      301 gtctcgttca ccgtcacctg gccgaaaatg gaggccgcga ccgctccggc cagcggcagg
      361 atcagccaac cttgcgcgaa ggacagggca agcatcgccg gcgccagcac caggctgatc
      421 ggcaggaaca ccttcttcag tgtgttgcgg tcgatcattc ggcccaccga gaactgtgtc
      481 agcgcgccgc acagggtcac caggaacgca gcggcgccga ccagtggcag cagttcggca
      541 ttatccgcca gcctctcctg cagcagcttc ggcagcagca aggtgaacgc gttgaacacc
      601 agccccgagg acgtcgcggt cagcatcagc accagcaccg ctcggcgcac caaatgacgg
      661 gggatcggcg gaaacgggcg tctgttggca tggatattcg cttgcgcgag cggcgttcgc
      721 atccacagca gcccggcggc ggcgcaaagc agtccagggg cgatgaaggc ggcgcgccag
      781 ccaaccgcat tcgccaggaa cgcggtgacg acaggggcca gcgctacccc gaggttgccg
      841 aacacgccgt tgacgccgat cgagcggccc ggcttgtcgc ccgctgcctc caccagcatc
      901 gtcgtgccga tcgggtggta gatcgccgcg aatccgccgg caacggcgag cgccaccgcc
      961 agcatcaccg gcgaatgggc caggcctgcc gcgatcagcc cgcccgccgt tccaaggaaa
     1021 aaagccgtca tcagcacctt gcggccgaag cgctgtgcca gccagccttg cggcagcgac
     1081 aacaggccgt agagcacgaa catgccggtc gcgagcgcca gaatcgggcc ataggactcg
     1141 ccaaatgggc cgtttggaac ggccatcgcc agtactgcgg ttggcaggat cagcaggcta
     1201 taatgggtga tcgtgtgcgc ggtgttgatg aacacaatct gccgcagggc cggggtcatc
     1261 acggatgttc cttgaacgcg tcattcgata taggctgtcg ttatgacgat gtctggccaa
     1321 atcccgttgc gagacggcca cagtctgctg cagccgtttg agcatgcgcc ttgctcgatg
     1381 gtcggatact cgcacgatta cttgtccggc cacaaaaccg gactgcaccg gtatccacgc
     1441 gcagttgctg tgcgcgaccg gcaccggtgg tgctcgccgc cgggcggagt ggtgccagat
     1501 catcgggaat acgggcacta tatcaccaac ccgccatcga catggattgc ctgaccggtg
     1561 acgtaggcgg cggcgggtga ggcgagaaaa gccactaccg cggcgacttc ttccggcagt
     1621 ccgatccggc ccagcggaat gcgcgccgtc tggtccgcga gcacgccttg gccaatcgcc
     1681 cggtgcgcac cctcgtcctt gcggatgaat cctggcacca cggcgttcac cgttatgccg
     1741 gccggaccaa gttcgatggc caatgcccgc accagcgcct ccaggccggc cttggcggcg
     1801 gccgaggcag ggaaggtggt caggtccgta cggaaagcat gggcgacgaa gctcgaaacg
     1861 gcgatcaccc ggccgtggct ggcgccgtgc aaatgtggca tcgccgcacg ggccaggcgg
     1921 aagaaggcgc cttggatcgt ctcggtcgag accgtcaacg cctgggacgt caggtcagcg
     1981 aaggcggtcc ggtcggcgaa gccggcgttg ctgatcagca cgtccaggcg gccgaattgc
     2041 tccacggttg ttgcaaccag tgcttccgcc acggccggtt cagccagatc gccgagggcg
     2101 accgccgcca gtccgccggc gtcgcggata ttccgggcta cggcttcggc gccttcgcgg
     2161 ttgcgccttg tatggacgag gatcgcggtt tcgggccgcg ccattgcacg acagaccgcc
     2221 gcgccgatgc cactggcggc gcccgtcacg agcaggatac gtcgttgttg catgagtggg
     2281 atgtccggca gtcgtggcaa gtgctgttgg acgttagtcc acgatcgcgg cgaggaaaat
     2341 ggtggctcgt ttggaatgcg gcgagtggtc ttgcgtgctg gccgaaaatt cgccacgtcc
     2401 gggcgacata gcgggagatc atgcgcctca tgcggcatct gtccgccgac gttcttgtta
     2461 ttggggccgg gggcgccggg atgtatgcgg cgatcgaggc cgcccgcgcc ggcgccaacg
     2521 tgctgctgct ggaccgtagc ctgatcggac gcggcggtgc gacgatcatg gcgcagatga
     2581 cggtcgccgc ggcggttgga ccgccgggca gcgaccattg gcgtcaccat ctggccgaca
     2641 cgctcgtggc aggccgtggt ttgtgcgatc ccgaactcgc gcgacttctg tgcgaagcgg
     2701 gtgtcgaagt cattcaggag atggagcgct ggaaggtcgg ctgggctcgc gacggagcgc
     2761 atctccgctc cgtccaggca cctggtcacg atcgtccccg ctgcgtgtac gtggatttcc
     2821 tgtccaccgg cccggccgtg agcaagacgt tgcgcggcgt cgtgcagcgg acgcctcgca
     2881 tcgaacggct tggggatgcg ctggtcaccg acctggtgac ccgggacggc gtggtgaccg
     2941 gtgccgtcgt ggttcacatc gaaaccggca aggtgctgac catcggtgca aatgcaacga
     3001 tcatcgcgac cggcggcctg acaaggctgt atcgccgcaa cagcgcgagc gccaatatgg
     3061 gcggtgatgg ctacgccctg gcgctgcggg ccggggcgga actgatcgac atggag
//
LOCUS       DASWQN010000848         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_82191, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000848 DASWQN010000000
VERSION     DASWQN010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82191"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VGC82_14345"
     CDS             complement(<1..241)
                     /locus_tag="VGC82_14345"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEY0424495.1"
                     /translation="MLTLRSGASSLVVAPEYGAGITGWMVGRTPILRRALPQALVGGD
                     SHAMGCFPLVPYGNRIAWGRFHWDGTEYVLQPNFGD"
     gene            305..1414
                     /locus_tag="VGC82_14350"
     CDS             305..1414
                     /locus_tag="VGC82_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0424496.1"
                     /translation="MVKVDCVVVGAGVVGLAVARALAMAGREVIILEAAEAIGTETSS
                     RNSEVIHAGIYYPANSLMARFCVAGRRQLYAYCAEKGVPHINCGKLIVATNPQEDAML
                     AGIKQRAETNGVEGMRVLTAAEAIALEPNLVCTSALLSPATGIVDSHGFMVALQGDAE
                     NAGAVSVFHSPVLGGRAGGRGIEIDVGGADPMSLRCRLLVNSAGLHAPGLAGRIAGMP
                     QSRVPTAYYAKGNYFTLSGRSPFSRLIYPVPVPGGLGVHLTVDLGGQARFGPDVEWID
                     TIDYTVDPSRSDSFYAAVRKYWPGLKDGALQPGYAGIRPKTVPKGAAGQDFVVQGPQS
                     HGLPGLINLFGIESPGLTASLAIADHVLEVSRTES"
     gene            1491..>2469
                     /locus_tag="VGC82_14355"
     CDS             1491..>2469
                     /locus_tag="VGC82_14355"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY0424497.1"
                     /translation="MFIRNAWYVAAWADEVAEQPLARRICNEPVVLFRDAEGRAAALL
                     DMCCHRGAPLHLGKVTERGLACGYHGLTFDHTGRCVYIPGQDRIPERASVRAFPVVER
                     DAFLWIWMGDAAKADPAAIVPYPWHNDTARWPHKHTMYPIKAPAMLMVDNLMDLTHLG
                     YVHTSTIGGNPTAHVEAKMETTRTPLGLKFTRWLPNSLPPPTYIKAVGFQNRIDRVQM
                     FEFIAPGSVLQLSAADEAGAYASGKVDDSKLQFRLFHGLTPETDTTCFYFWSAANGFR
                     PSDPAATEGLFNETSAAFKEDLIMVEAQQARLSQIGETALVDIATDAARL"
BASE COUNT          459 a          742 c          833 g          435 t
ORIGIN      
        1 ggtcgccaaa gttgggctgc agaacatact cggtgccatc ccagtggaag cgcccccacg
       61 cgatccggtt gccgtatggc acgagcggaa aacatcccat cgcgtgcgaa tcaccgccga
      121 ccaaggcctg cggcagcgcc cgccgcagta tcggtgtgcg gcccaccatc cagccggtaa
      181 taccggctcc atattcggga gcgaccacga gggatgatgc gcccgacctc agggtgagca
      241 tatatggact ccggttcgcg cccagcctaa cccgttcgat ggctcattgt caggaggaaa
      301 cagagtggtg aaagtcgatt gcgtggtggt gggtgcgggg gtggtgggac tggcggtggc
      361 gcgcgcgctg gcgatggctg ggcgtgaggt cattatcctg gaagccgccg aggcaatcgg
      421 gaccgaaacg tcatcgcgca acagcgaagt gatccacgcc gggatctatt accccgcgaa
      481 cagcctgatg gctcgatttt gcgtcgccgg gcggcggcaa ctgtatgcgt attgcgccga
      541 aaagggcgtg ccgcacatca actgcggtaa gctgatcgtc gcgaccaatc cacaggaaga
      601 cgcgatgctg gccgggatca agcagcgcgc ggaaaccaat ggcgtcgagg gcatgcgggt
      661 gctgaccgcg gccgaggcca tcgcgctgga gccgaacctg gtctgcacca gcgcgctgct
      721 gtcgccggcg accggaatcg tcgatagcca cggcttcatg gtcgcgctgc agggcgatgc
      781 cgaaaacgcc ggcgcggtct cggtattcca cagcccggtg cttggcgggc gggcgggcgg
      841 gcgtgggatc gagatcgatg tcggaggcgc cgatcccatg tcgttgcggt gccgcctgct
      901 ggtgaactcg gctggtttgc acgcacccgg attggcggga cggatcgcag gaatgcccca
      961 gagtcgggtg ccgacagcgt attatgccaa ggggaattac ttcacgctgt ccgggcgttc
     1021 gccgttctcg cggctgatct acccggtgcc ggtgccaggc ggtctcggtg tccacctgac
     1081 cgtcgatctg ggagggcagg cccggtttgg ccccgacgtg gaatggatcg acaccatcga
     1141 ttacacggtc gatccctcac gttcagacag tttctacgcg gcagtgcgca agtactggcc
     1201 agggttgaag gatggcgcgc tacagccggg atacgccgga atcaggccga agacggtgcc
     1261 gaagggcgcg gcggggcagg atttcgttgt gcagggcccg caaagccatg gcttaccagg
     1321 gctgatcaac ttgttcggga tcgagagtcc gggcctgacc gcctcgctgg cgatcgccga
     1381 tcatgtgctg gaggtttcgc ggacggaatc ataggcttcc ggggcggagc ttgacagcgg
     1441 tggccgcttg cgtcggatca tgcggggaaa ggatacaggg aggggaaaaa atgttcatcc
     1501 gcaatgcctg gtacgtcgct gcctgggccg acgaggtggc cgaacagcca ttggcccgga
     1561 ggatctgcaa cgagcctgtg gtgctgtttc gcgatgccga gggccgcgcc gcggcgctgc
     1621 tcgatatgtg ctgccaccgc ggcgcgcctt tgcacctggg aaaagtgacg gaacggggtc
     1681 tcgcctgcgg ctatcacggc ctgaccttcg accacaccgg acgctgcgtt tacatccccg
     1741 gtcaggatcg cattccggaa cgggcgtcgg tccgagcgtt tccggtggtg gagcgggacg
     1801 ctttcctgtg gatctggatg ggtgatgcgg cgaaagccga cccggccgcg atcgtgccct
     1861 atccctggca caacgacacc gcgcgctggc cgcacaagca cacgatgtat ccgatcaagg
     1921 ccccggcaat gctgatggtg gacaatctga tggacctgac gcatctaggc tacgtccaca
     1981 cctcgaccat cggcggtaat ccaacggccc atgtcgaagc gaagatggaa accacccgta
     2041 caccgctcgg gctgaagttc acacgatggt tgccgaactc ccttccgccg ccgacgtaca
     2101 tcaaggcagt cggcttccaa aaccgcatcg accgggtgca gatgttcgag ttcatcgcgc
     2161 cagggtcggt cctgcaactc agcgcggcgg acgaagccgg tgcttacgcc agtggcaagg
     2221 tggatgacag taagctgcag ttccgcttat tccacggcct gaccccagaa accgacacga
     2281 cctgtttcta cttctggtcg gctgccaatg gtttccggcc gagcgaccct gccgctaccg
     2341 aagggctgtt caacgagacc tccgctgcct tcaaggagga tctcatcatg gtcgaagcgc
     2401 agcaagcgcg tttgtctcag atcggcgaga cagcgctggt ggatatcgcg accgacgcgg
     2461 caaggctgc
//
LOCUS       DASWQN010000849         3484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1136663, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000849 DASWQN010000000
VERSION     DASWQN010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136663"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VGC82_14360"
     CDS             complement(<1..486)
                     /locus_tag="VGC82_14360"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY0424498.1"
                     /translation="MNQILADVTRTLPPTDIELAAVVRNFHTARVLVIGDVILDRYIS
                     GAVHRLSPEAPIPVLRPATNQCTLGGAANVALNIATLGGQAILIGVIGDDAAGDEVRR
                     LVAATPGVASAMVRIAGRPTTSKTRFMTGSHQLLRLDEETTAPLDRAGVAAILEAVEQ
                     QL"
     gene            681..1076
                     /locus_tag="VGC82_14365"
     CDS             681..1076
                     /locus_tag="VGC82_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424499.1"
                     /translation="MKYLPPFELIRRSIEISTVRGELRVNLSYEDFVAILRQLIQGVE
                     VDEAWYLRAYEDIGGAVKEGAVGSARQHFVHDGYFEGRLPFPMAVDERWYLAENPDVA
                     ESIRHGMVQSAQDHFDKDGYREGRLPFGM"
     gene            1287..2048
                     /locus_tag="VGC82_14370"
     CDS             1287..2048
                     /locus_tag="VGC82_14370"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY0424500.1"
                     /translation="MDDAALLALAAELAARAGTEIMAIRKRGFQIDRKADQSVVTEAD
                     RAAEAIILAGLRRALPGCSVVAEEEASTGKIVAATPEFWLVDPLDGTREFSSGGNDFA
                     VNIGLVRAGRPVLGVVGVPATGEIFGGIVGSGAWRRKDGNRSPVVARMPPPAGLTVVA
                     SRYHGDQARLDGFLQGRPVAEVVNFGSSLKFLRLAEGKADLYPRFGRTMEWDTCAPQA
                     VLEAACGSVETLEGQPLRYGKPGWDNPHFVCWGLR"
     gene            complement(2139..2771)
                     /locus_tag="VGC82_14375"
     CDS             complement(2139..2771)
                     /locus_tag="VGC82_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424501.1"
                     /translation="MAKAILLVGFDYSNAHEDEFHDWYDLEHVPERRRVPGFGLCERW
                     ISVANPKHAVATYDLESLGVLKSEPYLAIAYENLSVWSKRVGPMCTRLIRFDGEQTLP
                     GDADSPAGAGGLLVNAMNVAPEHDAEFNEWYDNEHIPALAAVPGVLSARRFMDATGTH
                     RYLALYHLKAPEVAMGPDWKNAASSPWTERLRPHFKDHLRILTRRYVRTP"
     gene            3006..3428
                     /locus_tag="VGC82_14380"
     CDS             3006..3428
                     /locus_tag="VGC82_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HEY0424502.1"
                     /translation="MLHLTKLAVGVRDADHLRELQASRLRERSPLRHRTRNFPRRRTE
                     VVDGGSIYWVIAGTMMARQRITDIIEDRRDDLTPCTSLILDPELVPLVGRPTRPFQGW
                     RYLDPDAAPPDMAALGTILGLDSLPASLRQELRALCLL"
BASE COUNT          638 a         1141 c         1089 g          616 t
ORIGIN      
        1 gagctgctgt tcaaccgcct ccaggatcgc cgccacgccc gcccgatcaa gcggcgccgt
       61 ggtctcctca tcaaggcgga gaagctggtg cgagcccgtc atgaaccggg ttttggaggt
      121 cgtcggccgg cccgcaatcc ggaccatggc ggaagcgacg cccggggtag ccgcgacaag
      181 ccgtcggacc tcgtcacccg ccgcgtcgtc gccgatcacg ccgataagga tcgcctggcc
      241 acccaatgtc gcgatgttca gcgcgacatt ggccgccccg ccgagggtgc actggttcgt
      301 cgcaggccgc agcaccggaa ttggcgcctc cggcgacagg cggtggactg cgccggaaat
      361 gtaacgatcc aggatcacgt caccgatcac caggacccgt gccgtgtgga aattccgcac
      421 gaccgcggcc agttcgatat cggtgggcgg cagggtgcgc gtaacgtctg ccaggatttg
      481 gttcatacgg ggcaccggtc ggttgatagc aaaggagccg ctgaatagca ggatgattcc
      541 aatttcgcac ctgctttcgc acctgaccgc actgggagat taaataccga atctgattcc
      601 atgtcggtcg cggaagcgct attggagcgg gaccggcgaa agacgacgcc cgacacaagc
      661 agcccgatca tggagacacc atgaaatatc ttccgccttt cgaattaatc cgccgctcaa
      721 tcgagatttc aacggtccgc ggcgaacttc gggtcaacct ttcctatgag gattttgttg
      781 ccatactgcg gcagctgatc cagggcgtcg aggtggatga agcgtggtat ctgcgcgcct
      841 acgaggatat cggcggcgcc gtgaaggaag gcgctgtcgg ctccgccagg cagcattttg
      901 ttcatgatgg ctacttcgag ggacgcctgc cgtttcccat ggcggtggac gagcgatggt
      961 atctggctga aaatccggat gtcgccgaaa gtatccgcca cggtatggtg cagtcggcgc
     1021 aggaccattt cgacaaggac ggttaccgcg aaggccgcct gccatttggc atgtgatcga
     1081 atgccgttcg ggcagcctaa tgacttcgga cagcctaatg acgatgcgtc acccccgctt
     1141 cccaaaccca cgcccaaacc catgcccaaa ccgacgatag tgtatgagac cgcgaccatg
     1201 atgcacaggc caacgatccg ccgtagcaag gcaaggaacc cagcggccga acgcgatcac
     1261 ccgcatcgct cgggcgtgcc ggcatgatgg acgatgccgc gctcctggcc ttggcggccg
     1321 aactcgcggc tcgggcgggg acggaaatca tggccatccg aaaacgcggg ttccagatcg
     1381 accgcaaggc ggaccaatcg gtggtgaccg aggcggaccg cgcggccgag gccatcatcc
     1441 tcgccgggtt gcgccgcgct ctgcccggct gttccgtcgt cgccgaggag gaagcgtcaa
     1501 ccggcaagat cgtcgccgcg acgccggagt tctggctggt cgatcccctg gacgggaccc
     1561 gtgaattcag cagcggagga aatgatttcg ccgtcaacat cggtctggtt cgcgccggcc
     1621 gtccggttct cggtgtcgtc ggtgtcccgg ctaccggcga gatcttcggc ggtattgtcg
     1681 gctccggcgc ctggcggcgg aaagacggca accgttcgcc ggtcgtggcc aggatgccgc
     1741 cgccggcggg cctgacggtc gtcgccagcc gttaccacgg cgatcaggcg cgactggacg
     1801 ggtttctcca agggcggccc gtcgcggagg tcgtcaactt cggctccagc ctgaaattcc
     1861 ttcgcctggc cgaaggaaaa gccgatctgt atccccgctt cggccgcact atggagtggg
     1921 acacctgcgc gccgcaagcg gtgcttgaag ccgcttgcgg ttcggtcgaa acgctggaag
     1981 gccaaccgct gcgctacggg aaacccggct gggacaaccc ccatttcgtc tgctggggcc
     2041 ttcggtaacc cgcccccgat cctgaacccg ccacggtcct gaacccgccc cgagccacaa
     2101 cccgccctta gccacaagcc acaacctgcc cgcggccctt aaggcgtgcg gacgtagcgg
     2161 cgcgtcagga tacgcaggtg atctttgaaa tgcggccgca accgctcggt ccagggcgat
     2221 gacgccgcat ttttccagtc cggacccatc gccacctccg gcgccttaag gtggtaaagc
     2281 gccagatacc gatgggtccc ggtcgcgtcc atgaaacgcc gcgccgacaa cacgccggga
     2341 accgcggcaa gcgccgggat gtgctcgttg tcgtaccact cgttgaattc agcgtcgtgt
     2401 tcgggggcca cgttcatggc gttcaccagc aagcctccgg cgccagcggg actgtcagcg
     2461 tcgcccggca aggtctgctc gccgtcaaag cggatcagcc gggtgcacat cgggccgacg
     2521 cgcttcgacc agacggacag gttctcatag gcgatggcga ggtacggctc gctcttgagc
     2581 acccccaggc tttccagatc gtaggtcgcg acagcgtgtt tgggattggc aacactgatc
     2641 caccgctcgc ataggccgaa accgggcaca cgccgccgtt ccggcacatg ttcaaggtcg
     2701 taccagtcgt ggaattcgtc ctcatgcgca ttcgaatagt cgaagcccac cagcagaata
     2761 gcttttgcca tgttcccctc catcttctgg attccggtgc cggcagcatg atcgggtgaa
     2821 accgatcgga cgccaagggt cctgcgacac ggtcatgcga caccatcccc ggcgccggaa
     2881 cgcccatccc gagatgctgc gccgcatcgt aaaatctgcg gcggtctggc ttgacggggt
     2941 gaagcgtccc atgtttgcgc ctacactggc atgccgaaaa cgcccagaac ccgagtcgcc
     3001 tcatcatgtt gcatctgacc aagctcgccg tgggcgttcg agacgccgat cacttgcgcg
     3061 aattgcaggc gtcgcgcctg cgcgagcgtt ccccactgcg ccaccgcacc cgcaacttcc
     3121 cgcgccggcg gaccgaggtc gtggacggcg gctcgatcta ctgggtgatt gccgggacga
     3181 tgatggcgcg gcagcgcatc accgatatca tcgaggatcg gcgcgacgat ctgacgccct
     3241 gcaccagcct gattctggat ccggaactcg tgccgctggt cggccgccca acccgtccgt
     3301 tccagggctg gcgctacctc gatcccgatg ccgccccgcc cgacatggcc gcccttggca
     3361 cgattctagg tctggacagc ctgcctgcgt cactgcggca ggaacttcgg gcgctctgtc
     3421 tgctgtgatc tcgcgcaggg attcagcttc atgacgggca gatgaatcag gttcatgtga
     3481 tcgg
//
LOCUS       DASWQN010000850         3766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_477443, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000850 DASWQN010000000
VERSION     DASWQN010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477443"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VGC82_14385"
     CDS             <1..531
                     /locus_tag="VGC82_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019087800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEY0424503.1"
                     /translation="SAVTYLEGYLFDPPAAQSAFRKAAAAAHAAGRRVALSLSDAFCV
                     NRHRAAFLDLVANHVDILFANETEITALYQRNSFEEAVAAVRQDVALAALTRSEEGSL
                     ILHGNETIHIAAQPTKVVDTTGAGDAYAAGFLVGLTSGKHLDLCGRMGSIAAAEIISH
                     YGARPEADLRKLMPPA"
     gene            complement(641..1636)
                     /locus_tag="VGC82_14390"
     CDS             complement(641..1636)
                     /locus_tag="VGC82_14390"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0424504.1"
                     /translation="MTTQPKLEHRPLGKAGLSVSAIGLGCMSLSGVYGEADDAASEDL
                     VRHAIDIGVDHFDSSDMYGWGHNEQVLGRALKGRRDGVVVATKFGQTQRPGQGNGVNG
                     RPEYVIEACEASLKRLGIEVIDLYYQHRVDPAVPVEETVGAMARLVEQGKVRFLGMSE
                     AAPDRIRRAHAVHPIAAVQTEYSLLYREEAEQTRATTVELGIGFVAYAPLGRGFLTGA
                     IKSFADVDGRRAAHPRFQEQNFDTNRSLVAKIEEIAKQKSCTPSQVTLAWLLAQGPDV
                     VAIPGTRYASRLDENVGALMVKLSAEDVARISAAVPAGAAAGTRYPAGGMAGVFI"
     gene            1821..2864
                     /locus_tag="VGC82_14395"
     CDS             1821..2864
                     /locus_tag="VGC82_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0424505.1"
                     /translation="MQTSHLPVLSYPFSTPPEPGTLTDVAPGIRWLRLPLPYRLDHVN
                     VYLIENAGGWTALDTGLGTDACKQAWEAALSGPLRGQGLKSLIVSHFHPDHVGLAGWL
                     CQRYGLELTMPRPEYLHSLVLQCAPADYGEEVFRPFYQRHGLSAEATEIVLSRGHEYL
                     KRTTGVPASYHRIKHDDTLAVGPRDFKVLTGGGHALEQAMLYRPEERLFLAADQVIAR
                     ISPNVSVHPMEPDLDALGIYLASLRAIKASVAPDVLVLPGHGLPFHGLHVRVEQLIEH
                     HALRCGEIAVACKDTPKSVAEIVPHLFTRELDAHQTGFAFGEVLAHVNHMLGRGELRL
                     ETDAGHVDRYRTV"
     gene            complement(2931..3362)
                     /locus_tag="VGC82_14400"
     CDS             complement(2931..3362)
                     /locus_tag="VGC82_14400"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY0424506.1"
                     /translation="MAQIKRHASAKWQGSGKEGSGSLTTQSGTLKDTPYGFNARFGDG
                     KGTNPEELIAAAHAGCFSMATAFQLSGAGHPPESMDCEANLTMEQVPGGWKIAAIHLV
                     LKARVPGLDDAKFQELARGAKENCPVSKVLNAEITLEASLV"
     gene            complement(3455..3702)
                     /locus_tag="VGC82_14405"
                     /pseudo
     CDS             complement(3455..3702)
                     /locus_tag="VGC82_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
BASE COUNT          671 a         1266 c         1163 g          666 t
ORIGIN      
        1 tccgccgtca cctacctgga aggatacctg ttcgatccgc cggcggccca atcggcgttc
       61 cgcaaagccg cggcggcggc ccacgccgcc ggccggcggg tggcactatc attgtccgac
      121 gcgttctgcg tcaaccggca ccgtgcggcc tttctcgatc tggttgccaa tcatgtggac
      181 atcctgttcg ccaacgagac cgaaatcacc gcgctgtatc aacgcaacag cttcgaggaa
      241 gccgtcgcgg ccgttcgaca ggatgtcgcc ttggcggcac tcacccgcag tgaggaaggc
      301 agcctgatcc tgcatggcaa cgagacaatt catattgccg cccagcccac gaaggttgtg
      361 gatacgacgg gggccggcga tgcctacgct gccgggttcc tggttgggct gacatcgggc
      421 aagcacctgg acctgtgcgg ccgcatgggc agcatcgccg cagccgaaat catcagccac
      481 tacggcgctc ggccggaagc cgacctacgg aaactgatgc ccccggcgtg aggccacaag
      541 atcaacctcg tatcgtggcg ctacggtggc ccccggccaa tgacgcgccg gcggcgccag
      601 cggaagccgc gatgggccgc cgaggcataa ctgcaccggc ttagatgaac acccccgcca
      661 ttccgcccgc cgggtatcgc gtcccggcag cggcacccgc gggcaccgcc gccgaaatac
      721 gtgccacgtc ctcggctgac agcttcacca tcaaagcacc gacattttcg tccagccggg
      781 acgcgtagcg cgtgccggga atggcgacca cgtccggacc ttgcgccagc agccaggcca
      841 gggtgacctg cgatggggtg caggacttct gcttcgcgat ctcttcgatc tttgccacca
      901 acgaccggtt ggtgtcgaag ttctgctcct ggaaccgggg atgtgccgcc cgccgtccat
      961 cgacgtcggc aaagcttttt atcgctcccg tcaggaagcc ccgccccaga ggcgcgtagg
     1021 cgacaaagcc gatccccagt tccaccgtgg tcgcccgcgt ctgctcggct tcctcgcgat
     1081 acagcagcga gtattccgtc tgcaccgcgg cgatcggatg caccgcgtgg gcgcggcgga
     1141 tgcggtccgg cgccgcctcc gacatgccga ggaaccgcac cttgccctgt tccaccagcc
     1201 tggccatcgc tccgacggtt tcctcgactg ggacggccgg atcgacgcgg tgctggtagt
     1261 acaggtcgat gacttcgata ccgagccgct tcaggctggc ttcgcaggcc tcgatgacat
     1321 attcaggccg cccattgacg ccgttcccct gccccggccg ttgcgtctgg ccaaacttgg
     1381 tggccaccac cacgccatcg cgccggccct tcagcgccct gccgagcacc tgctcgttat
     1441 ggccccaacc atacatgtcg gacgagtcga aatgatccac gccaatgtcg atcgcatggc
     1501 gaacgaggtc ttccgatgcc gcgtcgtcag cctcgccgta aacgcccgac agcgacatgc
     1561 acccaaggcc aatcgccgag acggacaggc cggctttgcc aagcggacga tgttcgagct
     1621 taggctgcgt tgtcatggga atagtcctcc aatgccgaat ttagcagcat tatggagtgc
     1681 cggtcgggat gacgccagag tgggccgccc caaagtggac cgcgccagag tgggccgtcc
     1741 agagtgcgcg ccaatattat ggacccgatc cgggaccgtt tgccatatcc gagcggcggc
     1801 ggttttgcta ccaagcccgg atgcagactt cacatctccc cgtcctgagc tatccgttct
     1861 ccactccccc cgaaccaggc actttgaccg acgtggcgcc cggcatacgc tggctgcggc
     1921 tgccgctccc ttatcggctg gatcatgtga acgtttatct gatcgagaac gccggcggct
     1981 ggaccgcgct cgataccggg ttgggcacgg atgcctgcaa gcaggcgtgg gaagccgcgc
     2041 tatctggccc attgcgggga caaggcctga aatcgctgat cgtgtcgcat ttccatcccg
     2101 accatgtcgg gttggccggc tggctgtgcc agcggtatgg cctggaactg acgatgccgc
     2161 ggccggaata tctgcacagc ctggtgctgc aatgcgcgcc ggcagattac ggtgaggaag
     2221 tgttccgccc gttctaccag cggcacgggc tgtcggccga ggcgaccgaa atcgtcctga
     2281 gccgcggaca tgaatacctg aagcgcacga cgggggtgcc cgcctcgtat caccggatca
     2341 agcacgacga cacgctggcg gtgggcccgc gcgacttcaa ggtgctgacc ggcggcggac
     2401 atgcgctgga gcaggcgatg ctctaccggc cggaggaaag gttgtttctc gcagccgacc
     2461 aggtcatcgc ccgcatttcg ccgaatgtca gcgtccaccc gatggagccc gacctcgacg
     2521 cgctgggcat ctatctggcc tcgctgcgag ccatcaaggc aagcgttgcg cctgatgtgc
     2581 tggtgctgcc gggacacggc ctgccgttcc acggcttgca tgtccgggtg gagcaattga
     2641 tcgagcacca tgcgttgcgc tgtggagaga tcgcggttgc ctgcaaggac acacccaaat
     2701 cggtggcgga gatcgtgccg catctattta ccagggaact cgatgcccat cagaccggct
     2761 tcgcgttcgg cgaggtcctg gcgcatgtga accacatgct gggccgcggc gagttgcggc
     2821 tggaaacgga tgccggccac gtggatcgct accggacggt gtaacgggtt cggaaggacg
     2881 atccgaccct gagttgccgg atcgcctacc cgatgacctt gcgccggcgc ctaaaccagg
     2941 ctcgcctcca gcgtgatctc ggcattcagc accttggaaa ccgggcagtt ctccttcgcg
     3001 ccccgcgcca gctcctggaa cttggcatcg tccagtccag gcactctggc cttcagcacc
     3061 aggtggatcg ccgcgatttt ccagcccccc ggcacctgct ccatcgttaa attggcctca
     3121 caatccatgc tttccggtgg atgccccgcg ccgctgagct gaaacgcagt cgccatgctg
     3181 aagcagccgg cgtgcgcagc cgcgatcagc tcctccgggt tggtgccttt gccatcacca
     3241 aaccgggcgt tgaagccata cggagtgtcc ttcaaagttc cgctttgcgt cgtcaggctg
     3301 cccgagcctt ccttgccgga cccctgccac ttcgccgacg cgtgccgttt gatctgagcc
     3361 atagcgtttt ctccttcatg atagcctggc gcccgccctt ccggccgccg aggaaacgta
     3421 aatcgcgctg taagcccgct gggcgcggga agcctcagcg ggtgcgttga gcgtcgatgc
     3481 tccagatccc gcccccggcc gcggcaaggt agaggaatac gaagcaaaac agtatggccg
     3541 cgtcaccgcc attcaggacc gggaagaaat tccgcgggta gtgagacatg aaataggcga
     3601 cggccatgtc gccgccagga tgaacgccac cggacgcgtg aacagtccga ggatgatcag
     3661 gatgccaccc accacctcca ggcagccttg cacccccagc agcgacatca gctcgggggc
     3721 ggcgggtgcg gtacgcggtg gaatcgagaa aagcgtctgc gttccg
//
LOCUS       DASWQN010000851         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_740584, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000851 DASWQN010000000
VERSION     DASWQN010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740584"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..296
                     /locus_tag="VGC82_14410"
     CDS             87..296
                     /locus_tag="VGC82_14410"
                     /inference="COORDINATES: protein motif:HMM:NF043052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HEY0424507.1"
                     /translation="MVDSVYKIIDLVGSSETSISKAIDRAISKAGESVRQLGWFEVTQ
                     IRGRIESGKIGSYQITLKAGFKLED"
     gene            323..1243
                     /locus_tag="VGC82_14415"
     CDS             323..1243
                     /locus_tag="VGC82_14415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0424508.1"
                     /translation="MAADASAVGTAQAVRGCLYGVLAALIWGSQLVMSRAGVTVGLDG
                     FDVAAIRCGAAGLVMLPWFMIQHPYGRGAGRTAWSHAVWLALAAGPLFTFASLGGFRF
                     APLPHGAVLQPSAMVLGSMLLSTLVLGERWRPLRLLGAAVIVLGLILIAGPALLQGDR
                     LTPIGDLSFMTAGLLFATYSVLQKRWSVGPMAATGATSLVAAVFYVPFYLAFAKWERI
                     LALSWPMLIAQIVVQGLLTGFVSLVAFGRSIQILGAARASLFPALVPASAIVLGIPVV
                     GEWPNWGQTAGLVVVTIGLLIALSSGVKRA"
     gene            1433..1591
                     /locus_tag="VGC82_14420"
     CDS             1433..1591
                     /locus_tag="VGC82_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424509.1"
                     /translation="MRPHANSAAIADALAFLAVQPNRLDPLMRAQRVLAPGVNAALLP
                     YGCHPNPE"
BASE COUNT          241 a          496 c          540 g          357 t
ORIGIN      
        1 tatgcgtccg attacaccat agccggacga gcgtagggtg aacctctcgc agctgaagcg
       61 agggtcaaaa cgggggggat agggctatgg ttgacagcgt ctataagatc atcgatttgg
      121 ttggatcatc cgaaaccagc atcagcaagg ccatcgacag ggccatctca aaggcaggcg
      181 agagtgttcg tcagctcggc tggttcgagg tgacgcagat ccgaggccgc atcgaaagcg
      241 gcaagatcgg cagctaccag atcaccctca aagccggctt caagttggaa gattgaggcg
      301 aatgggctga ggctggctga cgatggcagc cgacgcgtcc gcggtcggaa ccgcacaggc
      361 tgtcagagga tgtctgtatg gcgtgctggc cgctctgatc tggggcagtc aactggtgat
      421 gtcgcgggcg ggtgtcaccg tggggctgga tggcttcgat gtggcggcca ttcgttgcgg
      481 cgcggcggga ttggtgatgc tgccgtggtt catgatccag catccctatg gacgtggtgc
      541 aggacgcacc gcctggtcgc atgcggtttg gctggctctg gccgcggggc cgctgttcac
      601 cttcgccagc ctgggcgggt tccgctttgc tcccttgccg catggcgccg tcctgcaacc
      661 gtcggcgatg gtgctgggat cgatgctgct atcgacattg gtcctgggcg agcggtggcg
      721 gcctttgcgt ctgctcggcg cggcggtcat cgtgctgggc ctgatcctga tcgccggacc
      781 ggcccttctg cagggcgacc ggctgacgcc catcggcgat ctgtcattca tgaccgccgg
      841 cctgctgttc gcgacctatt ccgtcctgca gaagcgctgg tcggtcggcc caatggctgc
      901 cacgggcgcg acaagccttg tggccgctgt cttctacgtg ccgttctatc tggccttcgc
      961 caaatgggag cggatcctgg cgttgtcctg gcccatgctc atcgcccaga tcgtggtgca
     1021 gggcctgctg acgggctttg tctcgttggt ggcctttgga cgttccattc agatcctggg
     1081 tgcggcacgc gcctcgctgt tccctgcgct ggtgccggct tcggccatcg ttctggggat
     1141 accggttgtc ggcgagtggc cgaactgggg ccagacagcc ggcttggtcg ttgtgacgat
     1201 cggcctgctg attgctctca gttcaggtgt taagcgtgcg tagcgcgcgg tcgaacgtcg
     1261 cgccgtgatc ctctacccca atatgccttt gcggattctc cgcatcgctg ccgtcatctc
     1321 ccggcgccgc tcaatcgctt cggccgatcg cagtctgtgc ttccacatcc cgatagcgac
     1381 tggccttgag ggccatcaac cagtctgcgc cttgatggcg cggtgaacca cggtgcgccc
     1441 gcacgcgaac agcgcggcga tcgccgatgc tcttgccttc ctcgcggtgc agccgaaccg
     1501 cctcgacccg ctgatgcggg cgcagcgtgt gcttgcgccc ggtgtgaacg ccgcgctgct
     1561 gccctacggt tgtcacccaa atccggaatg acttgctaat ccctcggttt tccgaatcga
     1621 tgcatcgatc gatt
//
LOCUS       DASWQN010000852         3644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_740585, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000852 DASWQN010000000
VERSION     DASWQN010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740585"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1305)
                     /locus_tag="VGC82_14425"
     CDS             complement(121..1305)
                     /locus_tag="VGC82_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424510.1"
                     /translation="MWENRCKVAISGVGFSKVSRTADIPLAAHALTAVKEAVADSGLQ
                     MSDIDGLATYPELPATGHAEVDGISIVSVNCMMAMLKLPDLTWHIQVGTTNIGGAVQQ
                     AANALLAGVCKYAVVWRAMHNPRGTYQNLPGAQAHGAAQFTAPYGFGGPGQGMAVAYT
                     RWLERNNQSRDKMATLAVTQRRHTQHNPHAYFHGTPLTHEDYFSSRMVAYPFCLFDCD
                     IPVQGAVAIVLTTAERARDLKPKPAFLAGYGQRLHFEVAGRIGSLSSYMEGGSSSAKL
                     TWERSGFSPKDVDVAQIYDGFSASVIYGLESYGFCKEGEALDFIQDGRIELDGDLPLN
                     TFGGSLGTGRIHGLWHIIEGALQASGRAGSRQVKDANVSFVGASAPIVTGTTFIFVGD
                     PY"
     gene            complement(1319..1597)
                     /locus_tag="VGC82_14430"
     CDS             complement(1319..1597)
                     /locus_tag="VGC82_14430"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY0424511.1"
                     /translation="MGIDLKFEAVRGEGAIYAYTIMYHTGDKRFASAVPYASIVVELD
                     DAPNALLAGNLLEAPYTDAKVGRRVEVIFEKLNDDITLPQFRLARTAE"
     gene            2041..2748
                     /locus_tag="VGC82_14435"
     CDS             2041..2748
                     /locus_tag="VGC82_14435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEY0424512.1"
                     /translation="MTDPGHLKSSIKICMFDQYGTVVDMQGGLRDFAAGFLRAKGWAG
                     DPNAFVTWWRRTHFENSMIDALLQHEHTSYREIGHLSVRQVMDRADIAHTEDDVRSLV
                     AAIERLQCFPEVPAALARLRTRYKLVVLSNGDPDMLEAAKQHHGIPFDAVISVAEANA
                     FKPHRATYEMAARRMNTAMNEILFVANHEFDCVGAKAAGMSTVFIDRRRRPFARWPHQ
                     PDMIVNSMTELADLLVG"
     gene            complement(2869..3318)
                     /locus_tag="VGC82_14440"
     CDS             complement(2869..3318)
                     /locus_tag="VGC82_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0424513.1"
                     /translation="MTKGFLLDTNVISAAAPDRRTVPDAAKAAAREWIAENQGRLWLP
                     VTAITEIAAGIGTREGAGGSRHAADLAGWLKAVLGAYSNRVLMLNAEAALHARTLSRN
                     AREAGIAVGFADLTVACIASAHGLVVATRNIRDFTPMGIETLDPFTA"
     gene            complement(3315..3554)
                     /locus_tag="VGC82_14445"
     CDS             complement(3315..3554)
                     /locus_tag="VGC82_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY0424514.1"
                     /translation="MHELQLRDAKASFSAVVEQAAKGEETVVTRHGQPTAVVLGYEVW
                     QRLTNSRPSFADLLLSFPDVGEVVRDRTPARDSDL"
BASE COUNT          702 a         1180 c         1071 g          691 t
ORIGIN      
        1 acgaaaaggc aagataaccg acctgggact cgggcaagtt cgcgcgaaac ggcgagcgcc
       61 gatcggctaa gcctcacgtt ccccgcaatc gccggagtgc cgccggcctg cttccagccg
      121 tcagtacgga tcacccacga aaataaacgt cgtgccggtc acgatggggg cgctggcgcc
      181 gacgaaggag acattggcat cctttacctg ccgcgatccg gcgcggcccg atgcttgcag
      241 cgcgccctcg atgatgtgcc aaagcccgtg gatacgtccg gtgcccaggc tgccgccaaa
      301 agtattcagc ggcagatcgc catcaagctc gatgcgcccg tcctgaatga aatccagcgc
      361 ctcgccttcc ttgcagaacc cgtaggactc caacccatag atcacagatg cggaaaaacc
      421 gtcatagatc tgggccacgt caacgtcctt tggactgaag ccggagcgtt cccaggtcag
      481 cttggcggag ctgctgccgc cctccatgta gctggacagg ctgccgatgc ggcccgccac
      541 ctcgaagtgc agccgctgcc catagccggc caggaaagcc ggcttcggct tcaggtcccg
      601 cgcccgctcc gccgtggtca gcacgatggc gactgcccct tgcaccggga tgtcgcagtc
      661 gaacaggcag aacggatagg cgaccatccg cgacgaaaaa tagtcctcat gcgtcagcgg
      721 cgtgccgtga aaataggcgt gcggattgtg ctgcgtgtgg cggcgctgcg tgaccgccaa
      781 ggtcgccatc ttgtcgcggc tttgattgtt ccgctcaagc cagcgcgtgt aggcaaccgc
      841 catcccctgc cccggaccgc cgaaaccata gggcgcggtg aactgggctg caccgtgcgc
      901 ctgggccccg ggaaggttct gataggtccc gcggggatta tgcatcgccc gccacaccac
      961 ggcgtatttg catacccccg cgagaagtgc gttcgccgcc tgctgcaccg cgccgccgat
     1021 gttggtcgtt cccacctgga tgtgccaggt caggtcaggc agcttcaaca tcgccatcat
     1081 gcagttgacc gacacgatgg agatgccgtc cacctccgca tgcccggttg ccggcaattc
     1141 cggataggtc gccaaaccgt cgatgtccga catttgcagt ccggaatccg cgacagcctc
     1201 tttcaccgcc gtcagcgcat gagctgcgag tgggatgtcg gcggttcggg aaaccttgga
     1261 gaaacccacg ccgctgatgg ccactttgca ccggttttcc cacatgcgat ttctcccatt
     1321 attcggcagt acgcgccagg cggaactgcg gcagcgtgat gtcgtcgttc agtttttcga
     1381 aaatgacctc cacgcgccgc ccgaccttcg catcggtata aggcgcttcc aacaggttgc
     1441 cggcgagcag cgcgttcggc gcgtcgtcga gctccacgac gatgctggcg tagggcacgg
     1501 cggaggcgaa gcgcttgtcg ccggtatgat acatgatggt gtacgcgtag atcgcacctt
     1561 cgccgcgcac ggcttcgaac ttcaggtcga tccccatgca ttggacgcac gtcgcatacg
     1621 gcggatgctg aaaatggccg caagtctgac agcgttgcag ttcaagtgcg ccccgtttgg
     1681 cggcatccca aaacggtttt gtccactcgt tcgggaccgg gatcggccgg gttaccttgc
     1741 tccagtcgct cgttgtcatc gccttgtccc tccggttcgt ctgatagcaa cggggcgcct
     1801 tggtggcatc caaatggcaa cactcaatgc tgcttcccga tcagtctgcc gatcttgact
     1861 tggttgtggc cagcaccgcc tatttctcaa tggtaaagat gatcgtgaac gccgtcatgc
     1921 agcctggaaa ggatcaccgg aacccatcgc tccaaaagga ttgcgaaccg ccgtccggtt
     1981 tgaggctgcg tgataggagt aggtttgtaa aaatgctcta ccacgaaatg gaccacgcgg
     2041 atgacagacc ccggccacct gaaatcgtcc atcaaaatat gcatgttcga ccaatacggt
     2101 accgtcgtgg acatgcaggg tggactgcgc gacttcgccg ccgggttcct gcgggcaaag
     2161 ggctgggccg gcgaccccaa cgcatttgtc acatggtggc gccgtaccca ttttgagaac
     2221 tccatgatcg acgccctgct gcagcacgag catacgtcat accgcgaaat tggccacttg
     2281 tcggtccgcc aggtgatgga ccgagccgac atcgcccata ccgaggatga cgtgcggtcg
     2341 ctcgttgcgg ccatcgagcg cctgcagtgc ttccccgaag tgccggcggc gctggctcgc
     2401 ctccggaccc gctacaagct tgtggttctg tcgaatggcg acccggatat gctcgaggcc
     2461 gcgaagcaac accacggtat tccattcgac gccgtcattt cggttgccga ggcgaatgcc
     2521 ttcaagcccc atcgagccac atacgagatg gccgctcgga ggatgaatac cgcgatgaac
     2581 gaaatcctgt tcgtcgccaa ccacgagttc gactgcgtcg gcgccaaggc cgccggcatg
     2641 tccacggtct tcatcgaccg ccgtcgccgc ccatttgccc gctggccgca ccagccggac
     2701 atgatcgtca actcgatgac cgaacttgcc gatctcctgg taggctaacg tcccggttca
     2761 gatcggcgct ggtatgtcgg gcggccagtg cctcgtccgc gggggcgggt agccgccaca
     2821 ccaaagccaa cggtcaatgg cagggacttt attcgattcg atctctgctc atgctgtgaa
     2881 cggatccagc gtttcgatgc ccatcggcgt gaagtcccgg atgttgcggg tagcaaccac
     2941 caatccgtgg gcgctcgcaa tgcaagccac ggtcaggtcc gcgaagccaa ctgcgatgcc
     3001 ggcctctcgc gcgttgcggg aaagagtccg tgcgtgcaac gctgcttccg cattcaacat
     3061 cagcacccgg ttcgagtacg cccctagaac agccttgagc caaccagcca aatccgccgc
     3121 atggcgcgac cctcccgcac cctcgcgcgt accgataccg gcggcgattt ccgtgatggc
     3181 ggtaacaggc aaccagaggc gaccctgatt ttcggcgatc cattctcgcg cagccgcttt
     3241 tgcggcgtcg gggacagttc tccgatccgg tgcggcagcc gaaatcacat tggtatcgag
     3301 cagaaagccc ttggtcacag gtcgctatcg cgcgccggtg tccggtcgcg caccacttcc
     3361 ccgacatcgg ggaaactgag caggagatcg gcgaaggagg ggcggctgtt ggtcagacgc
     3421 tgccaaacct cgtagccaag cacgaccgca gtcggctggc catgccgagt gaccacggtt
     3481 tcctcacctt tcgccgcctg ctcgaccacg gcggagaaag acgccttggc atcccgcaac
     3541 tgaagttcgt gcatcgcttt tcctgacgtg accattgtgg tcacagtcat atagcagcga
     3601 ccatgccgaa taccaacact ttctggcacc cctgtcggta gcac
//
LOCUS       DASWQN010000853         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_82341, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000853 DASWQN010000000
VERSION     DASWQN010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82341"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..>1715
                     /locus_tag="VGC82_14450"
     CDS             339..>1715
                     /locus_tag="VGC82_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEY0424515.1"
                     /translation="MHEVMKQGLYDPKNEHDSCGVGFIVNIKGRKSHDIISQGLKILE
                     NLNHRGAVGADPLVGDGAGILLQMPDALLNDWARANGKSLPAVGRYAVAMCFLPQDQA
                     ARDHVIEHFEQVVAKEGQAMVGWRDVPLDITGLGKTVIETMPVIRQAIVASSLSIRDQ
                     NAFERKILAIRKQILNNLRTLAEKRNIPGVTETYMPSFSTRTIVYKGLLLATQVGTFY
                     RDLGNPLTTSALAMVHQRFSTNTFPSWKLAHPYRFLAHNGEINTVRGNVNWMYARRRS
                     MESDLIGADLNKMWPIIPHNQSDTACIDNALEILVAGGYSLAHAMMMLIPEAWAHDPL
                     MDPERKAFYEYYAALMEPWDGPAAIAFTDGRQIGATLDRNGLRPARYVITDDDYVIMA
                     SEAGVLPVLEEKIVRKWRLQPGKMLLIDLEEGRIIDDAEIKQKLAREHPYAEWLKETQ
                     FKLEDIPES"
BASE COUNT          359 a          543 c          523 g          290 t
ORIGIN      
        1 atattggatg gcggtaccat cgcacgggcg cgaaccatgg ggaagccgac gcggtcggtg
       61 ctgccgccac cgccgccgga ggcaagtgcg gccatccggg ttcgccgtca cgcctgaagg
      121 cggaccattg cggcgaaccg gcagcggaaa accgggttcc gaagggtggg gacaggggcc
      181 tttcgggtcg cttccaccgc ttgccgttca cggcacattt agcttacgct gcgatgcggc
      241 aggatcattg ccatttgccc gccgaacggg cagactggtc cattgtgccc aatcgcgcat
      301 tttgagggca aatccgatac gccagggaag ctgtggcaat gcatgaggtt atgaaacaag
      361 gcctttacga ccccaaaaac gagcatgatt cctgcggcgt tggcttcatc gtcaacatta
      421 aaggccgaaa atctcatgac ataatctccc agggcctgaa aatcctggag aacctgaacc
      481 accgcggcgc agtgggtgcc gatccgctgg ttggcgacgg cgccggcatc ctgttgcaaa
      541 tgccggacgc attgctgaac gactgggcgc gggcgaacgg caagtcactg cccgccgtcg
      601 gccgctatgc cgtcgccatg tgcttcctgc cccaagacca ggccgctcgg gaccacgtga
      661 tcgaacattt cgagcaggtg gtcgccaagg aaggccaagc catggtgggc tggcgcgatg
      721 tcccgctgga catcaccggc ctcggcaaga cggtcatcga gaccatgccg gtcatccgcc
      781 aggcgatcgt ggcctccagc ctgtcgatcc gcgaccagaa cgcgttcgag cgcaagatcc
      841 tggcgatccg caagcagatc ctgaacaacc tgcggaccct ggcggaaaag cgcaacatcc
      901 ctggcgtgac cgaaacctat atgccgtcgt tctccacccg gacgatcgtt tataaggggc
      961 tgttgctggc gacccaggtc ggcacgttct atcgggatct cggcaacccg ctgacgacat
     1021 cggctttggc gatggtacac cagcgctttt ccaccaacac cttcccgtcg tggaagctgg
     1081 cgcatccgta tcgcttcctg gctcacaacg gcgaaatcaa caccgtccgc ggcaacgtca
     1141 actggatgta cgcccgccgc cggtcgatgg aatccgacct gatcggcgcc gacctgaaca
     1201 aaatgtggcc catcatcccg cacaatcagt cggacacggc ctgcatcgac aacgccctcg
     1261 aaatcctggt cgccggcggc tactcgctgg cgcatgcgat gatgatgctg atcccggagg
     1321 cctgggcgca cgacccgctg atggatcccg aacggaaagc gttctacgag tactacgccg
     1381 cgctgatgga gccatgggac ggccccgccg cgatcgcctt taccgatgga agacagatcg
     1441 gcgccaccct ggaccgcaac ggcctgcgcc cggcgcgcta cgtcatcaca gacgacgatt
     1501 acgtcatcat ggcttcggaa gccggcgtgc tgccggttct ggaggagaag atcgtccgca
     1561 aatggcggct gcagcccggc aaaatgctgc tgatcgacct ggaagaaggc cgcatcatcg
     1621 acgatgcgga gatcaagcag aagctggctc gtgagcaccc gtacgcggaa tggctgaagg
     1681 agacgcagtt caagctggag gacatccccg aaagc
//
LOCUS       DASWQN010000854         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2590059, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000854 DASWQN010000000
VERSION     DASWQN010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2590059"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(545..1822)
                     /gene="serS"
                     /locus_tag="VGC82_14455"
     CDS             complement(545..1822)
                     /gene="serS"
                     /locus_tag="VGC82_14455"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY0424516.1"
                     /translation="MHDIRAIRADPAVFDAAMARRGLPPVSVELMKLDADRRAAQTAL
                     QERQARRNALAREIGQGKRSGADTAALEVEATALRGDMDGLEKSAAELDTAIRGILET
                     LPNVLDPSVPDGADETANVVLKQVGQPRGFDFNPKQHFELGEDLKMMDFATASKLAGA
                     RFTVLRGPLARLERALGQFMLDQHTQERGYEETIVPSLVNAATAYGTGSLPKFEEDLF
                     KTTDGRYLIPTAEVPLTNVVAGDIVPEKQLPIRLTALTDCYRSEAGSAGRDTRGMLRQ
                     HQFRKVELVCIAHPDNSSVEHEWMTRSAELILEKLGLPYQRVVLASGDTGFASTRTHD
                     LEVWLPGQAMYREISSCSNCKDFQARRMNARFRGGADGKTIAHVHTLNGSGVAVGRAL
                     IAVMENYQQADGSIVVPDVLRPYMGGMASIGGL"
     gene            complement(1827..>2051)
                     /locus_tag="VGC82_14460"
     CDS             complement(1827..>2051)
                     /locus_tag="VGC82_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY0424517.1"
                     /translation="QLPVALTLLAKVGITNSAQLKKFRRYAYVGMFVIAAILAPPDVI
                     TQTGLAVPLIALYEISIISAGWVETKPVED"
BASE COUNT          380 a          713 c          604 g          354 t
ORIGIN      
        1 cgaaacagag cgtgatcacg ctgtgcggaa cgtccgaagc gtgtgaatca tgcgagcaca
       61 ccaagaggta gggcattttc ggcctgcaca gccaggctga aatggtctgg acgcactggc
      121 ggcaccactt cgtcatgccg acgcagggcg gcatccacgc cgaccgccgt atgaccgaag
      181 tcgtggatga tggcgcctga tgcggctgtg tcggtctatc ggcggaccga cataaaccgc
      241 gtgacgacga accctgttcc cgcctgctac cgctcgcagc ggcccgaacc aacgtctcga
      301 tcatcgacga caaggcagga ctacttctcc gcgtcctcca cgacctccgt gcgctccgtg
      361 tcaagagcct tcgccagcct tcgcacgggg ctcaagccga acccgccgaa caccgcggcc
      421 caccgcacca tccgccaatg tcaaaacccg gcaggaacct gcctcggcat ccaactgcac
      481 tccacaccag gaacccctgc cacagcctca cgacccgaaa tctggcacta caccccatct
      541 ggcactacaa cccgccgatg cttgccatcc cgcccatgta agggcgaagc acgtccggca
      601 ccacgatcga tccgtccgcc tgctgatagt tttccatgac cgcgatcagc gcgcgcccaa
      661 cggccacgcc cgatccattc agcgtgtgaa catgggcgat ggtcttgccg tccgccccgc
      721 cgcggaagcg ggcgttcatc cgccgcgcct gaaagtcctt gcagttcgaa caggaggaga
      781 tttcccggta catcgcctgc ccgggaagcc acacctccag gtcgtgcgtg cgggtagagg
      841 caaatccggt atcgccggag gccagcacca cccgctggta cggcagtccc agtttctcca
      901 ggatcaattc ggcggaccgc gtcatccact catgctccac cgagctgttg tcggggtgag
      961 cgatacagac cagctccacc ttgcggaatt ggtgctggcg cagcatcccg cgtgtatcgc
     1021 gtccggcgga acccgcctcg ctgcggtagc agtccgtcag cgccgtcagg cgaatgggaa
     1081 gctgcttctc gggcacgata tcgccggcca cgacattcgt cagcggcact tccgccgtgg
     1141 ggatcaggta gcggccgtca gtggtcttga agaggtcttc ctcgaatttc ggcaggctac
     1201 cggtcccata ggcggtggcc gcattgacca gggacggcac gatggtttcc tcgtagcccc
     1261 gctcctgcgt gtgctggtcc agcatgaact ggccaagcgc gcgctccagc cgggcaagcg
     1321 gcccacgcag tacggtaaac cgggcgccgg ccagcttgga ggccgtcgcg aagtccatca
     1381 tcttcaggtc ttcgcccagc tcgaaatgct gcttcgggtt gaagtcgaag ccgcgcggct
     1441 ggcccacttg tttcagcacc acgttggctg tttcgtcggc gccatcaggc accgaaggat
     1501 ccagcacgtt cggcagcgtt tccagaatgc cgcgaatagc ggtgtcgagc tccgcggcac
     1561 tcttctccag accgtccatg tcgccgcgca gcgcggttgc ctcaacctcc agggcggcgg
     1621 tgtcggcacc gctgcgcttg ccctggccga tttcccgcgc caaggcatta cggcgggcct
     1681 gcctctcctg caacgcggtt tgcgcggccc gccggtccgc atccagcttc atcagctcca
     1741 ccgagaccgg cggcaacccc cggcgcgcca tcgccgcatc gaaaaccgcc ggatcggctc
     1801 ggatggcgcg gatatcgtgc atggcgctag tcctctacgg gcttcgtttc gacccaaccg
     1861 gcgctgatga tggagatttc gtacaaggcg atcaacggaa ccgccaaccc ggtctgggtg
     1921 atgacgtccg gaggtgccag gatcgccgcg atcacgaaca tgccgacata agcgtagcgg
     1981 cggaacttct tcaactgagc cgaattggtg atcccgacct tcgccaaaag cgtcaacgcg
     2041 accggaagct g
//
LOCUS       DASWQN010000855         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2061911, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000855 DASWQN010000000
VERSION     DASWQN010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2061911"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VGC82_14465"
     CDS             <1..940
                     /locus_tag="VGC82_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424518.1"
                     /translation="GLPYLDAIEYRIVPNRSTRILGFVAGTFDMTYPTDVSVPLLRDI
                     QQQDPDAHCEMRRTNVSTNLIINRDAPPFDNPAIRRALALAIDRRAFNDIINEGQGRI
                     GAVMLPPPEGVWGMPEEMLHTLTGYSPDVTKNREEARGIMKQLGYGPDHLLKTKIFTR
                     NINGFRDPALILNDQLKQVFIDAELDVADTPQYYSRVFKKDYAVGLNLTGSSLDDPDQ
                     NFFENYACGSLRNYTNYCNPALEKEFEAQSVETDFAKRKAIVWDIERKLADDVVRPII
                     YHDVAAACWQPYVKNVTIMVNSIYNGWRWEDVWLDK"
     gene            1027..1791
                     /locus_tag="VGC82_14470"
     CDS             1027..1791
                     /locus_tag="VGC82_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEY0424519.1"
                     /translation="MTQKTKLRSSFQEAVADIPDGSTIAFGGFAMPGTPFNLIKALME
                     QGARRLTLVGNTTGGAQQPRMPDIGMLVENGQVAKVICAFTAATRPTDVLPFTKYYES
                     GQVEAELVPQGTLAERLRAAGAGIPAFYTPTAVGTELAQGRESRVIDGREYLLEYALK
                     LDYAFIRARTADTAGNLRFHRSQRNFGPVMATAARITIAEIDEPVVPAGSIDPDDVHT
                     SGIYVHRLVQVPPEPVGLWPTKRQARGQVTGQEPRK"
     gene            1788..2459
                     /locus_tag="VGC82_14475"
     CDS             1788..2459
                     /locus_tag="VGC82_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEY0424520.1"
                     /translation="MSGTKGLNRQQMADRVAREFQDGWIVNLGVGIPTMCSNTDIGER
                     DITYHSENGVIGYGAVAAEGHEDLNLVNAGGQNVTLKPGAAIVHHADSFGVIRGGHID
                     ATVMGAYQVSMTGDFANWKLKGQKGGGIGGAMDLAACAKRVFIILEHVTRDGSARLVT
                     ACDLPVTARGVVTLVATNYGLFEPAGSGFRLLEIAPGLTVEEIRAATGGTLTIADGLR
                     EIAGR"
BASE COUNT          539 a          799 c          833 g          463 t
ORIGIN      
        1 aggcctgccg tacctcgacg ccatcgagta ccgcatcgtg ccaaaccgct cgacacgaat
       61 actcgggttt gtcgccggca cgttcgacat gacataccca acagatgtct cggtgccact
      121 gctgcgcgac atccagcagc aggatcccga cgcccattgc gagatgcggc gcaccaacgt
      181 cagcaccaat ctgattatca accgcgatgc tccgccgttc gacaatccag cgatcaggcg
      241 cgcccttgcg cttgcgatcg accgccgtgc tttcaacgac atcatcaacg aaggccaggg
      301 acggatcggc gccgtcatgc tgccacctcc ggaaggtgtc tggggcatgc cggaggagat
      361 gttgcacacg ctgacgggct atagccccga tgtcacgaag aacagggaag aagcccgagg
      421 gatcatgaag caactgggct acggcccgga tcacctgttg aagacgaaaa tcttcacccg
      481 caacatcaat ggatttcgcg atccggcgtt aatcctgaac gaccaactca agcaggtgtt
      541 catcgatgcc gaactggacg ttgcggacac gccgcaatat tacagccggg tgttcaagaa
      601 ggactacgcg gtcggcctga acctgaccgg cagttcgctc gacgatccgg atcagaattt
      661 cttcgaaaac tatgcctgcg gctcgttgcg gaattacacg aactattgca acccggcgct
      721 ggagaaggaa ttcgaggcgc agtcggtaga aaccgatttc gccaagcgta aggcgatcgt
      781 gtgggacatc gagcgcaagc tggccgacga tgtcgtgcgg ccgatcatct accacgacgt
      841 cgccgccgcg tgctggcaac cctacgtgaa gaacgtcacg atcatggtca acagcatcta
      901 caatggctgg cggtgggagg atgtttggct ggacaaataa gctggccgac cgtccgccgg
      961 tcccgctcgc cttgacaccg cgtcccgcct gaacgttcat cggcgcgaca cgaagggcgg
     1021 gaaacgatga cccaaaagac caagctgcga agcagtttcc aagaggctgt ggccgacatc
     1081 ccggatggca gcaccatcgc gtttggtggc ttcgccatgc ccggaacgcc gttcaatcta
     1141 atcaaggcgc tgatggagca gggggcaagg cgcctgaccc tggttggcaa taccaccggc
     1201 ggcgcgcagc aaccgcggat gcccgatatc ggcatgctgg tcgaaaatgg acaggttgcg
     1261 aaggttatct gcgcctttac ggcagcgacc cgcccgacgg acgtgttgcc attcactaaa
     1321 tattacgaat ccgggcaggt cgaggccgaa ctggttccgc aaggcacgct ggctgaacgg
     1381 ctgcgcgcgg cgggtgcggg catcccggct ttctacacgc ccaccgccgt cggcaccgaa
     1441 ctcgcccagg gtcgggaaag ccgggtcata gacggccgcg aatacctgct ggagtacgcg
     1501 ttgaagctgg actacgcctt catccgagcc cgcaccgccg acactgccgg caacctgcgg
     1561 ttccaccgtt cgcagcggaa ctttggcccg gtcatggcga cggccgcacg catcacgatc
     1621 gccgagatcg acgaaccggt cgtgccggct ggcagcatcg atcccgatga cgtgcatacc
     1681 tccggtatct acgtccaccg cctggtgcag gtgccgccgg aaccggttgg cctgtggccg
     1741 accaagcggc aggcccgggg acaagttact ggacaggagc cacggaaatg agcggaacga
     1801 aaggcctcaa tcgccagcag atggccgacc gcgtggcgcg ggaatttcag gacggctgga
     1861 tcgtcaacct cggcgtcggc atcccgacga tgtgctccaa caccgacatc ggcgagcggg
     1921 acattaccta tcattccgag aacggcgtga tcggctacgg tgccgtcgcg gccgaggggc
     1981 atgaggatct gaacctggtc aatgccggcg gtcagaacgt cacgctgaag ccaggtgcag
     2041 ctatagtgca tcatgccgac agtttcgggg tgatccgcgg cggtcacatc gacgcgacgg
     2101 tgatgggagc ctatcaggtc tcgatgaccg gggacttcgc caactggaag ctgaagggtc
     2161 agaagggcgg cgggatcggt ggagcgatgg atctggcggc ctgcgccaaa cgggtgttca
     2221 tcatcctgga gcacgtcacc cgggacggca gcgcgcgctt ggtgacggcc tgcgatctgc
     2281 cggtgacggc tcgcggggtc gttactctgg tggcgacgaa ttatgggctg ttcgagcccg
     2341 ccggcagtgg gttccggctg ctggagatcg cgcctggcct gaccgtggag gagatccgcg
     2401 ccgcgaccgg ggggacgctg accatcgcgg atggattgcg ggagatcgcc gggaggtagc
     2461 atctggggta ggccggcccg ctcccctatc gcttcttgat gatgatcttg actttgatga
     2521 tcacgatcac cagtatagac gccaggaagt ttagttgttt ccgcatctct tcctccaggt
     2581 tgaagccggc ggggccgtcc ccgccggctt ctccattttg gcgacaacct atga
//
LOCUS       DASWQN010000856         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_346707, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000856 DASWQN010000000
VERSION     DASWQN010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346707"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VGC82_14480"
     CDS             complement(<1..812)
                     /locus_tag="VGC82_14480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0424521.1"
                     /translation="MMFPPILRLIAATTAIRLAFAQTTGLGVDESYMVTAGRVVSLGY
                     FDHPPASWWLSWGAAHLLGTDAPVAVRLPFILLFALSQFLVWRIGCLAADSRSGYWAA
                     VALNLSPVFGVTTGSWVLPDGPLDAALLGAALCLLHALPATGHRTSGWWAASGVCAGL
                     ALFSKYSAVLSLAGAACYLMVSPTHRRWLLRPEPYIAAVLAVLVFSPVLVWNASHGWA
                     SFTFQGDRAMGVRLRPLMPLQTLGGEALFVLPWLWVPMMFLLVRGFRRDAAWP"
     gene            811..1020
                     /locus_tag="VGC82_14485"
     CDS             811..1020
                     /locus_tag="VGC82_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424522.1"
                     /translation="MECGCFCCLQTFEAAEVIDWIDDGDTPLCPHCGIAAVLPGVTDL
                     VELFRLRERRFGPRAGVTLLPGDDT"
     gene            complement(1081..1566)
                     /locus_tag="VGC82_14490"
     CDS             complement(1081..1566)
                     /locus_tag="VGC82_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY0424523.1"
                     /translation="MTDIDGYEVLGLGLYFEDLPVGRKFRTIGRTLTEADLVSFIGVT
                     GMTEVLFSNTEFLRNESDIQQRVVPGAMVYAFAEGLLVHATMQHTGFAFLNMELDVKG
                     PTFADDTIHVEAEVIESRRSASRPNRGLVRTFNCVVKQDGSVAITYNPLRMVKAKGTG
                     T"
     gene            complement(1633..2388)
                     /locus_tag="VGC82_14495"
     CDS             complement(1633..2388)
                     /locus_tag="VGC82_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0424524.1"
                     /translation="MRGLSGRNVLLTGGASGIGRATAVRLVEEGCRVGILDIDEAGAA
                     ETVRRCGGQAWTRRTDITDRASVEAAVAAFEHEVGPADCLANIAGWDVIRNFLDSDVA
                     LWDKIIAINLYGPLHLHHVVLPGMVKRGFGRVVNVSSDAGRVGSSGEAVYSACKGGII
                     AFTKTVAREVARKGVTLNVLCPGPTDTPLFDSVKASSPDGAKIGEAMARAVPLKRIGQ
                     PEDYPGTIAFLLSDDAAYITGQTLSVSGGLTMS"
     gene            complement(2473..2667)
                     /locus_tag="VGC82_14500"
     CDS             complement(2473..2667)
                     /locus_tag="VGC82_14500"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0424525.1"
                     /translation="MEPCIEAFGVERCMFESNFPPDKQSGGYTELWNAFKRIAAGASA
                     AEKTALFSGTAARVYRMIVP"
BASE COUNT          545 a          944 c          859 g          476 t
ORIGIN      
        1 ggccaggcag catcgcgccg gaagcctcgg accagcagaa acatcatcgg cacccacagc
       61 cagggcagga cgaacaatgc ctcgcccccc agggtctgca gcggcatcaa aggacgaagc
      121 cgcaccccca ttgctcggtc cccctggaag gtgaaggacg cccagccatg cgaggcgttc
      181 cagacgagca cgggcgaaaa caccagaacg gccaataccg ccgcgatata gggctccggc
      241 cggagcagcc agcgccggtg ggtcgggctg accataaggt agcacgccgc gcccgccagg
      301 ctcagcaccg ccgaatactt ggaaaacagt gctagtccgg cgcatacccc cgaagccgcc
      361 caccagcccg aagtccggtg tccagtcgcc ggcaaagcat gcagcagaca cagcgctgct
      421 cccaacagcg cggcatcgag cgggccgtca ggcaacaccc agcttccggt cgtcacccca
      481 aacaccggag acagattaag cgcgacagcc gcccaatatc ccgatcggct gtccgccgcg
      541 agacagccga tccgccagac caggaactgc gacagagcga acaaaaggat gaatggcagg
      601 cgaaccgcca ccggcgcatc ggtacccagc agatgcgccg cgccccagga cagccaccaa
      661 cttgccggag ggtggtcgaa atagccgagg ctgaccacgc gtccggctgt taccatatag
      721 ctctcgtcca cccccaggcc ggttgtctgg gcgaaggcca gccggatcgc cgtcgtcgcg
      781 gcgataagcc gcaggattgg aggaaacatc atggagtgcg gctgcttctg ttgccttcag
      841 accttcgagg ccgcggaggt catcgactgg atcgatgatg gcgacacgcc gctatgcccg
      901 cattgcggca tcgcggccgt actgcccggc gtaaccgatc tggttgaact gtttcgcctg
      961 cgtgaacgcc gcttcggtcc cagggccggt gtgacgctgc ttcccggcga cgacacatag
     1021 gttgggaccg agggccagcc gcgggacgcc tcggcgcgga gcagcgcatc ctccgtccac
     1081 tcaggtcccg gtgcccttgg cttttaccat gcgcaatgga ttgtaggtga tcgcgacgct
     1141 gccgtcctgc ttcaccacgc aattgaaggt ccgcacaagc ccccggttcg gtcggctggc
     1201 gctgcggcgg ctttcaatga cctcggcttc gacatgaatc gtgtcgtcgg cgaatgtcgg
     1261 gcctttcacg tccagctcca tgttcaggaa ggcgaagccg gtgtgctgca tggtcgcgtg
     1321 caccagaagg ccctcggcga aagcatacac catcgcgccc ggaacgaccc gctgctggat
     1381 atcgctttcg ttccgcagga actcggtgtt cgagaacagc acctcggtca tgcccgtcac
     1441 gccgataaag ctgaccagat cggcttcggt cagcgtgcgg ccaatggtgc ggaatttccg
     1501 tccgaccggc aggtcctcga agtaaagacc gaggccaagg acctcgtagc cgtcgatgtc
     1561 cgtcatcctc gcttcctcgc catcgttatc caatacctgc gcgcgtcatt cacaatgatc
     1621 ctggggaacc cgtcacgaca tggtcaggcc gcccgacacg ctgagggttt ggccagtgat
     1681 atacgccgcg tcgtccgaca acagaaaggc gatggtcccg gggtagtcct ctggttgacc
     1741 aattcgcttc agcggcacgg cgcgggccat ggcctcacct attttggcgc cgtcgggcga
     1801 tgacgctttg acgctgtcga atagcggcgt gtcggtcgga cccgggcaca gcacattcag
     1861 ggttacgcct ttgcgggcga cttcccgtgc cacggtcttg gtgaaagcga tgatgccccc
     1921 cttgcaggcg gaataaacgg cttcacccga ggaacccacg cgcccggcat cggatgacac
     1981 gttcacgaca cggccgaatc cccgtttgac cattcccggc aatacgacgt gatgcagatg
     2041 cagcgggcca tacaggttga ttgcaatgat cttgtcccaa agtgccacgt cgctgtcgag
     2101 gaagttccgg atgacgtccc acccggcaat attggccagg caatccgccg gcccaacctc
     2161 gtgctcgaac gccgcgacgg cggcttcgac gcttgcccga tccgtgatgt cggtgcgccg
     2221 ggtccaggcc tgaccgccgc accggcgcac ggtttccgcc gcgccggcct cgtcgatgtc
     2281 gagaatcccg acacggcaac cttcctccac cagccgaacc gccgtggctc ggccgattcc
     2341 gctcgcgccg ccggtcagca agacgttccg gcccgagaga cctctcattg atagccgccc
     2401 tgaaggcgaa tgcgaattgc gcgggtcatg cgaggcctcc tggaccgaga tgcgccgttc
     2461 gttgccggtc cttcagggca cgatcatgcg gtagacacgg gcagcggtgc cgctgaacag
     2521 cgcggttttc tcggcggcgg atgcaccggc agcgatgcgc ttgaaggcgt tccacaattc
     2581 cgtatagccg cctgactgct tgtccggcgg gaaattgctc tcgaacatgc accgctccac
     2641 gccaaatgcc tcgatgcacg gttccacaga ttgccgtcag gcagcggcga gttcttccga
     2701 cgatggcgga atgtcacgtt cgtggaactc gaagccgaac gaggtcatgc cgacgccacc
     2761 gagcttgacg ttgacgttag ggcacttggc gaactccttg atgtccttct gccagccggc
     2821 aagg
//
LOCUS       DASWQN010000857         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1005713, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000857 DASWQN010000000
VERSION     DASWQN010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005713"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..1104
                     /locus_tag="VGC82_14505"
     CDS             331..1104
                     /locus_tag="VGC82_14505"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0424526.1"
                     /translation="MATWRDQVALVTGGGRGIGRAIARRLAREGASVCVNYLSRADAA
                     ESLAADINANGGRAIAVAADVADDAAVEGMVDRVNAAFGPVTILVNNAGVSHQATLDS
                     YDAERLAHMRQVNADGVIHTVRAVMTDMRRQKYGRIVNITSIAALGTALPGNAFYAAT
                     KAEAQILTLRFALELGPHGISVNAVAPGFIQTEGTRGPNDDAWKERSQRIAALAMLGR
                     VGEPDDVANAVAFFASPESGWVTGQVLAVDGGRMDYIGG"
     gene            1190..1690
                     /locus_tag="VGC82_14510"
     CDS             1190..1690
                     /locus_tag="VGC82_14510"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEY0424527.1"
                     /translation="MSAADAPVFEAFIAPHRSLTGKAAAFIIAGMLVFSSGIALRCWI
                     WGAWPVVALSLLDVPLLVLLLAINQRRARASELIILDQAGLTVIRTDPRGVRARNKLP
                     AAWLRIDLHNGPGIPRVVLSSRGRTCEVGAFLHESEKIALFDALRDGVYRVRNPRFDN
                     PQLREE"
     gene            complement(1697..2134)
                     /locus_tag="VGC82_14515"
     CDS             complement(1697..2134)
                     /locus_tag="VGC82_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0424528.1"
                     /translation="MGSERESRPSPIDVHVGSRIRLRRTLLGMSQERLGEALGLTFQQ
                     VQKYERGVNRVGASRLFDLSRVLDVPISFFFDDMPDSLSASFGGLPNRRSGGTTEPQD
                     PFGDDTLSRRETLELVRAYYRITDPSIRKRVFDLIKSMGPTDP"
BASE COUNT          481 a          740 c          806 g          528 t
ORIGIN      
        1 gctgccctgc atatacggca acgccacatt ggcgatcgtt tgatccaatg attgcaacaa
       61 ggtcgcacca atggctgacg ccgttatcat cgcacgatgc ggaacgttgt ccgcggctgg
      121 caagcttggg gccgaggcgg ccatggcgct acttctggtt aaggtcaggg attctgcctg
      181 acgacacatg cccgatcagg atgagcgtaa cgttcggtcg cggtcaattg ccagcgcgtt
      241 cgcctcctcg tccgggtgtt gtgaccgcgc cggttattat cttgggatag tgtgttgttc
      301 cgcgaatagt agcgttcttt ggaggcttgc atggcaacat ggcgcgatca ggtagcgctc
      361 gtgacgggag ggggtcgagg cattgggcgc gctatcgctc ggcggcttgc gcgggagggt
      421 gcatcggtct gcgtgaacta cctgtcgcgc gccgatgccg cggaatccct ggccgcggat
      481 atcaatgcga atggcggacg cgcgatcgcg gtggcggcgg acgtcgcgga cgatgcggcg
      541 gtggagggta tggtcgaccg ggtcaatgcc gcattcggac cggtgaccat cctggtgaac
      601 aacgccggcg tctcgcatca ggcgaccttg gacagttacg atgcggaaag actggcgcac
      661 atgcgccagg tgaacgcgga cggcgtgatc cacacagtac gcgccgtcat gacggatatg
      721 cgccggcaga aatacgggcg tatcgtcaat atcacctcaa tcgccgctct cggcaccgca
      781 ttgccgggaa atgccttcta cgccgccacc aaggccgagg cgcaaatact gaccttgcgg
      841 ttcgccctgg aacttgggcc gcacgggatt tcggtgaacg ccgtcgcgcc gggtttcatc
      901 cagacagaag gaacccgcgg gccgaatgac gatgcgtgga aggagcgatc ccaaagaatc
      961 gccgcattgg cgatgctcgg ccgcgttggc gagcccgacg acgtggcgaa cgcggtggct
     1021 ttcttcgcat caccggagtc aggttgggta acgggtcagg tgctggcggt cgacggcggg
     1081 cggatggact atatcggtgg ctgagccgaa tccgactgct tcactcggcg cgaccatatc
     1141 ctggctcagc ggttctggag gttttttccg ggtctgatag gatggtccga tgtcagccgc
     1201 ggatgcaccg gtcttcgaag ccttcatcgc cccccaccgt agcctgacgg gcaaagcggc
     1261 cgccttcatc attgccggca tgttggtttt cagctctggg atcgccctgc gatgctggat
     1321 ttggggcgcc tggccggtgg ttgcccttag ccttctggac gtccccctgc ttgtcctgct
     1381 tctggcgatc aaccagcgcc gggcgcgggc gagcgagctc atcattctcg atcaagctgg
     1441 gcttacggtt atccggaccg atcccagagg tgtacgggcc cgtaataagc tgccggcggc
     1501 ctggctgcgg atcgatctgc ataacggccc cgggattccc agggtcgtcc tgagcagccg
     1561 ggggcgcacc tgcgaggtcg gtgcttttct gcacgagtcg gagaaaatcg cgttgttcga
     1621 cgcgttgcgc gacggggttt accgggtcag gaatccgcgg ttcgataacc cgcagttgcg
     1681 ggaggagtag cggatgtcag gggtctgtgg ggcccatcga cttgataagg tcgaaaaccc
     1741 gcttgcggat cgaaggatcg gtaatccgat aataggcccg aaccagctcc agcgtttccc
     1801 gccggctgag ggtatcgtcg ccaaacggat cctgaggttc cgtggtgccg cccgaacgcc
     1861 gattcggcaa cccaccgaaa ctcgcggaca ggctgtcggg catgtcgtcg aaaaagaagc
     1921 tgatcgggac gtcgagaacc cgcgacagat cgaacaggcg cgacgcgcca acccgattga
     1981 cgccgcgttc atacttttgc acctgctgga aggtcagtcc cagagcttcg ccaagccgct
     2041 cctggctcat gcccagcagg gtgcggcgga ggcgaatgcg ggaccccaca tgcacatcga
     2101 ttggactggg acggctttct ctttcactgc ccaatacttg ttccgccaca gccatgcctg
     2161 gtaccccttt ccggtgggcc ttcctatatc gcgtccgcgc ttatcgcact atcggcgctt
     2221 cgtagcgtga aactgaaaac aaactatggt gcttcatcga ttcgtgcaac caaaaattgt
     2281 caaatggaca gaaatttatt gtcatctagt ttaaatttta tgagtttatt ccaaaatttg
     2341 gacattatgc cgcttttcgc ggccgtttac cgacaaggcc actgatcatg agtgccaagg
     2401 caacgcctcc aggtagtggt aagcccgacc gagcatacaa tgttggcgga agcttcgccg
     2461 gcaaccgcac aggcaaaata tcggtgatcc cagtctccac tcgcccgagt tcgtgccctt
     2521 tcgcatcgaa gccggcggat atgccggtat tggcc
//
LOCUS       DASWQN010000858         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1798115, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000858 DASWQN010000000
VERSION     DASWQN010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798115"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VGC82_14520"
     CDS             complement(<1..867)
                     /locus_tag="VGC82_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424529.1"
                     /translation="MRYLLALLLSACISFALIWAWIAAMPMAFMDPEYPSWRAKEVML
                     DRCDLGEVIILGDSRAAADILPTRLPFRTTNLAVGGGEAIEAFSVMTRVLECHSLPRM
                     VILSLDAGHFTRPDLFWERSVRFGFLSATDIEGLRQASHQTGDPSVYEARPVEGLPPV
                     LRDWLYRIHFPPLYFSSLAHGGGIFRWVRNQDRLAATLSARGQYYFGTKAGSNTVAVE
                     GELESFHPLPILDFYFDKLLSELDRRGIQTRFIAMPINDATWERVRPVMRTGFAAYLA
                     GYQRRYRNFLVEP"
     gene            complement(867..>2150)
                     /locus_tag="VGC82_14525"
     CDS             complement(867..>2150)
                     /locus_tag="VGC82_14525"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEY0424530.1"
                     /translation="YGASAAAFLPLLILSVCGNFILLRAMHLSLVAGRWAAFGVPLNL
                     LVLGWFKYGGVDPLPPLGLSFFTFTQIGCLLHHAAGDTRPPKLRDFALFAAFFPALLA
                     GPILNPREMLPQFARTEDWQFTAGNIAVGSGFFLIGLLKKTLLADPLSTIVTTGFADP
                     AHLTLFPAWQATTAYSLRLYFDFSGYTDMAIGLAWMVGLRFPDNFQQPYRAASIIAYW
                     QCWHMSLTRFLMTNVHAPLTMAIMRRRRDHLRPIDAAGQRTIAGFLAMIAAPVGVTMV
                     LVSLWHGATIPFLLFGLLHTLFLLINHAWRLAGKAVLPQAASVVLTYLCVLAGSVFFR
                     ATSASDAASMLAGMAGLHGTGAIEPDIRAAVDVVWLVSLYAIVWLAPTTRQWMQGEAR
                     FAWKPSPRWAVAMGCAATLGLLAAGGSSEFLYFRF"
BASE COUNT          488 a          673 c          678 g          311 t
ORIGIN      
        1 gggttcaacc aggaagtttc ggtaccggcg ctggtagccc gccaggtaag ccgcgaaacc
       61 ggtgcgcatc acgggtcgca cccgctccca ggtggcatcg tttatcggca tggcgatgaa
      121 gcgcgtctgg atgccgcgcc gatccagctc cgacaacagt ttgtcgaagt aaaagtccag
      181 gatcgggagg gggtggaacg attccaactc accctcgacg gcgacggtgt tcgaaccggc
      241 cttggtgccg aagtagtatt gtccgcgcgc cgacaacgtc gccgccaacc tgtcctggtt
      301 ccgcacccaa cggaaaatgc cgccgccatg cgcgaggctg gaaaaataca gcggcggaaa
      361 gtgtatccgg tacagccagt cacgaagcac gggaggcagt ccctcgaccg gccgcgcttc
      421 atagaccgaa ggatcgccgg tctggtggga cgcctggcgc aggccctcga tgtccgtcgc
      481 cgaaaggaac ccgaagcgga cgcttcgttc ccagaacagg tcaggccggg tgaagtgccc
      541 tgcatcgagc gaaaggatga ccatcctggg caacgaatga cattcgagta cccgggtcat
      601 taccgaaaat gcttcaatcg cctcaccgcc gccaaccgcg aggttcgtgg tacggaacgg
      661 caaccgcgtg ggcaggatat cggctgccgc cctggagtcg cccaggatga tcacctcgcc
      721 cagatcgcac cgatcgagca tgacctcctt ggcacgccag gacggatact cggggtccat
      781 gaacgccatc ggcattgccg cgatccaggc ccagatcagc gcgaagctga tgcaggcgga
      841 cagcagcagg gcaagcagat agcgcatcag aaccggaaat acaggaattc gctgctgccg
      901 ccggcggcga gcaagcccaa cgtggcggcg catcccatcg ccacggccca tcggggagag
      961 ggtttccacg cgaagcgcgc ctcaccctgc atccactgcc gcgtagtcgg ggccagccat
     1021 acgatggcat acagcgacac cagccaaaca acgtccaccg ccgcacgaat gtcgggttcg
     1081 atcgccccgg tgccgtgcaa tcccgccatt ccggccagca tcgacgcggc gtccgaggca
     1141 ctcgtggccc ggaagaaaac cgaaccggca agaacgcaaa ggtaggtcag aacgacactc
     1201 gcagcctgcg gcaggaccgc ttttccggcg aggcgccagg catggttgat cagcaggaac
     1261 agagtatgca gcagcccgaa caacagaaac gggattgtcg cgccatgcca taacgagacc
     1321 agcaccatcg ttactccgac cggagccgcg atcatggcca gaaatcccgc gatcgttcgc
     1381 tgtcccgcgg catcgatcgg ccgcaaatgg tcccttcgcc ggcgcatgat cgccatcgtc
     1441 aacggcgcat gcacgttcgt catcaggaag cgcgtcagcg acatgtgcca gcattgccag
     1501 taggcgatga tcgaagccgc gcggtaaggc tgctggaaat tgtcgggaaa ccgcaggccg
     1561 accatccacg caaggccgat cgccatgtcg gtatacccgg agaagtcgaa atacagccgc
     1621 agcgaataag cagtggtagc ctgccatgcc ggaaacagcg tcaggtgggc gggatcggcg
     1681 aacccagtgg tgacaatggt ggaaagcgga tcggccagca gcgtcttctt cagcagtcca
     1741 atcaggaaaa acccgcttcc cacggcgata ttgcctgcgg tgaactgcca gtcttcggtg
     1801 cgggcgaact gaggcagcat ttcccgcgga ttcaggatgg ggccggcgag cagggccggg
     1861 aagaaggctg cgaacagcgc gaaatcgcgg agtttcggcg gccgggtgtc gcccgccgca
     1921 tgatgcagca ggcagccgat ctgggtaaac gtaaagaaac tgagtcccag cggcggcagc
     1981 gggtccaccc caccatactt gaaccagccc agaaccagca ggttgagcgg taccccaaac
     2041 gcggcccatc ggccagcgac gagggacaag tgcattgctc ggagcaggat gaaattgccg
     2101 cagaccgaca ggatcagcag cggcaggaat gcagcggcac tggcgccgta
//
LOCUS       DASWQN010000859         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_214610, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000859 DASWQN010000000
VERSION     DASWQN010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214610"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VGC82_14530"
     CDS             <1..801
                     /locus_tag="VGC82_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEY0424531.1"
                     /translation="RDVPVAQPPFWGARVIESAPKAIVPFVNERSLYQFQWGYRKQGR
                     TFEDFLGWAKQELRPVMRRMLTLCEDQEILRPQAAYGYWKAAGQGNDLVIFEQDGTTE
                     VARFALPRQPKDDGECIADFFRDIDDAERDVIALQVVTVGQKASDTARIWFEDNRYQD
                     YLYLHGLSVEMAEAMAEYVHKRIRAELGFAGEDDRDMEKMLSQGYRGSRYSFGYPACP
                     RLEDQVPILGMLGADRIGISLSDEFQLHPEQSTSAIVVLNPRAKYFSV"
     gene            804..1283
                     /locus_tag="VGC82_14535"
     CDS             804..1283
                     /locus_tag="VGC82_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY0424532.1"
                     /translation="MLDGNSDDTIREILTTTRRIALVGASAKSWRPSNGVMRFLLDRS
                     YDVTPVNPGLAGQTIHGRTVVGSLAEAAPLDMVDVFRASVHVGELVERVIALGARTIW
                     MQLGVVDHDAAVKARAAGIMVVMDRCPVIEDRRIGPFGTGLLSPTGPTQSNGPYSVQ"
     gene            1366..2232
                     /locus_tag="VGC82_14540"
     CDS             1366..2232
                     /locus_tag="VGC82_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 family protein"
                     /protein_id="HEY0424533.1"
                     /translation="MTNEERDIITKFIARVGGAAPSSGFGSGSVPATQPALPPIDRDA
                     DAFIGQAFTQYPEARYRITQLAFVQEHALVEAQNRIQRLEWELQQARQAAQQAQQSGA
                     RQGGGGFFSGLFGGGNRSQSAPPQGDPRWGGGGQGGGYQGGPQPNYQQSAPPPPQYPP
                     NYQQGMFAQRGGSGFLGSALTTAAGVAGGVVAGNALMGLFSPHQGLGGGFGGGGGLGG
                     SPWATPTGATQDTSGWGQGGGAAGNQDFVDNGTWDQPDTSTDSSWTDNSGGGSDSGWD
                     SGGGGGSSDDTF"
BASE COUNT          432 a          753 c          828 g          416 t
ORIGIN      
        1 cgggacgtgc cggttgccca acccccattc tggggcgccc gcgttattga atcggcgccg
       61 aaagccatcg tgccgttcgt caacgaacgc agcctgtacc aattccagtg gggatatcgc
      121 aaacaggggc gaacgttcga ggacttcctc ggctgggcca agcaagagct gcggccggtg
      181 atgcgccgca tgctgacgct gtgcgaggac caggaaatcc tgaggccgca agccgcctac
      241 ggctactgga aagccgccgg ccagggcaac gacctggtca tcttcgagca ggacgggacg
      301 acggaggtcg cccgcttcgc actgccgcgc cagccgaagg acgacggcga atgcatcgcc
      361 gacttcttcc gggatatcga cgacgccgaa cgcgatgtta tcgccctgca ggtggtgacc
      421 gtgggccaga aggcctcgga caccgcgcgc atctggttcg aggataaccg ctaccaggac
      481 tatctgtact tgcacggcct gtcggtggag atggccgagg cgatggcgga atacgtccac
      541 aagcgcatca gagctgagct gggctttgcc ggcgaagacg atcgcgacat ggagaaaatg
      601 ctgtcgcaag gctatcgcgg cagccgctac tcgttcggct atcccgcctg cccacgcctg
      661 gaggaccagg taccgatcct gggcatgttg ggggcggatc ggatcggcat ctccctgtcc
      721 gacgagttcc agttgcatcc ggagcaatcg accagcgcga ttgttgtgtt gaatccgcgc
      781 gcaaagtact tttctgtgta ggcatgctcg atggcaactc cgacgacacg atccgtgaga
      841 tcctgaccac cacgcggcgc atcgctctgg tcggtgcttc ggcgaagtcc tggcggccgt
      901 cgaacggcgt catgcggttc ctgctcgacc gctcgtacga cgtgacgcca gtcaaccccg
      961 gcttggccgg ccagaccatc cacggccgca cagtggtcgg ctcgctggcc gaagcggcgc
     1021 cgctggacat ggtggatgtg ttccgagcct cggtgcatgt cggcgaactc gtggagcgtg
     1081 tcatagcgct tggcgccagg accatttgga tgcagctcgg tgttgtcgac cacgatgccg
     1141 cggtaaaagc ccgagccgcc ggtattatgg tcgtgatgga ccgctgtccg gtcatcgagg
     1201 atcgccgcat cggcccgttc ggcaccggct tactcagtcc aaccggccct actcagtcca
     1261 acgggcctta ctcagtccaa tagtacccac cattgaacca aaaccgaatc ggcgccacat
     1321 tcgatctgtt cgggtcgttc tgacagaccc ataggggatg ccccaatgac gaacgaagaa
     1381 cgcgacatca tcaccaaatt catcgcccgt gtcggcggtg ccgcgccgtc ctcgggcttc
     1441 ggttccggtt cggttccggc aacgcagccg gcgctgccgc cgatcgaccg agatgccgac
     1501 gccttcatcg gccaggcctt cacccagtat ccggaggcgc gttaccgcat tacccagctt
     1561 gcgttcgtgc aggagcatgc gcttgtcgaa gcgcagaatc gcattcagcg gctggagtgg
     1621 gagctgcagc aggcgcgcca agccgctcaa caggcgcagc aatccggcgc gcggcagggc
     1681 ggcggaggat ttttcagtgg gctgttcggc ggtggaaacc ggtcgcaatc cgccccgccg
     1741 caaggcgatc cgcgctgggg cggaggcggc cagggcgggg gatatcaggg cggcccgcaa
     1801 ccgaattatc agcaatcggc accgccgccg ccgcagtatc cccccaacta ccagcagggc
     1861 atgttcgccc agcggggcgg gtcagggttc ctggggtcgg cgctgacgac agcggccggc
     1921 gtggcgggtg gtgtggtcgc aggcaatgcg ctgatgggct tgttctcccc gcaccagggc
     1981 cttggcggcg gttttggcgg tggaggggga ctgggtggca gcccgtgggc gacgccgacg
     2041 ggggctactc aggacaccag cggctggggg cagggcggtg gagcggccgg caaccaggac
     2101 tttgtcgaca acggcacctg ggatcagccg gataccagca cggactcgtc atggaccgac
     2161 aacagcggcg gcggcagcga tagcggctgg gacagcggtg gcggtggtgg gtcgtcggac
     2221 gatactttct agatccgtcg gttgccgcat gacgcagatc gtggcgcttg cgtcgttcca
     2281 ggcgatatca tggtgaaagt ccgctgcttc ggcggtggtt aaaacgtgac acggacaccg
     2341 ccataaccgg cgagcggcgg cccggcggcc aggctgcgcg tgttcgtggt attgggcgcc
     2401 tgggtgatcg gcaccaggga taccggcga
//
LOCUS       DASWQN010000860         3325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2062258, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000860 DASWQN010000000
VERSION     DASWQN010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062258"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1319)
                     /gene="odhB"
                     /locus_tag="VGC82_14545"
     CDS             complement(27..1319)
                     /gene="odhB"
                     /locus_tag="VGC82_14545"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEY0424534.1"
                     /translation="MATEIVVPALGESVTTATVARWMKQQGETVAADEPLVELETDKV
                     TVEVNAPAAGVLSSVAVPEGTEVEVGSVLGMLDAAGAAASTPAAASKSAPIAKPATTP
                     GPRPAANPPPGVNPPPRPLGPVSRPAASAPVHPPLPAAARMIAENRVSASDVGTGTGK
                     DGRITKGDVMDFLNRVAAPAPNAQAAPVAVKAPRQDEPREQRVKMTRLRRTIALRLKE
                     AQNNAAMLTTFNEVDMSAIMTLRSQYRDAFEKKHKGVRLGFMSVFVRACCVALKEFPA
                     VNAEIDGDDIVYKNFVNMGIAVGGPSGLVVPVIRDADQLGFAGIEAAIADFGKRARDG
                     ALKLDDLTGGTFSITNGGIYGSLMSTPILNPPQSAILGMHKIQERPVVIAGKIEIRPM
                     MYLAVSYDHRIVDGKEAVSFLVRVKEGVEDPRRLLLDL"
     gene            complement(1372..>3325)
                     /locus_tag="VGC82_14550"
     CDS             complement(1372..>3325)
                     /locus_tag="VGC82_14550"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY0424535.1"
                     /translation="VKYHLGTSTDVEIAGHNVHLSLQPNPSHLEAVDPVVVGKVRARQ
                     DRAGDTKMRGSVMAILMHGDAAFAGQGLVYETLAMSQLIGYRTGGTIHLVVNNQIGFT
                     TVPAHAYSGLYCTDVAKSIQAPILHVNGDDPEAVIFCARMAAEYRMKFASDIVLDIVC
                     YRRHGHNETDEPAFTQPLMYKAINATKTTRTLYAEKLAAAGVVPAEESRQMLDEFTKT
                     LEDANSAAKSYKPNKADWLEGHWSGFTPVDQQEIELTEEATAVPADTLKQIGAALAHV
                     PENFNLNPKIARQLEAKQKAIATGEGIDWATGEALAFGSLLLEGSRVRLSGEDTQRGT
                     FSQRHAVLIDQLNQHEYVPLNNIVSDQARIEIYNSLLSEAGVMGFEYGYSLADPRTLV
                     LWEAQFGDFANGAQVIIDQFLASGETKWLRMSGLVLLLPHGYEGQGPEHSSARIERYL
                     QLCAERNMYVCNPTTPANYFHALRRQLHRNFRKPLIVFEPKSLLRHKLAVSPLADMAE
                     GTRFQFVIPEVDELNPPEQIRRVVICSGKVYYDLLQERRSKDIKNIVILRLEQLYPFP
                     VNTLTRLLAPYTNADVVWCQEEPENMGAWNFVDRRIEKVLLRLDMKVRRPTYVGRDAA
                     ASPATGLARTHMAQQARLVATVLGIG"
BASE COUNT          580 a         1060 c         1035 g          650 t
ORIGIN      
        1 gccacagcga tgctccaaat ccactctcat aaatccagca gcagccggcg cgggtcctcg
       61 accccctcct tgacgcggac caggaaggac accgcctctt ttccgtccac gatgcggtga
      121 tcgtacgaca ccgccagata catcatcggc cggatctcga tcttgccggc gatcacgacc
      181 ggccgctcct ggatcttatg catccccaga atggccgact gaggcgggtt caggatcggc
      241 gtggacatca gcgacccgta gatgccgccg ttggtgatgc tgaaagttcc gcccgtcagg
      301 tcatccagct ttaatgcacc gtcacgcgcc cgcttgccga aatcggcgat ggcagcctcg
      361 atcccggcga accccaattg gtcggcatcg cggatcaccg gaacgaccag cccggagggg
      421 ccaccgacgg caatgcccat gttgacgaag ttcttataga cgatgtcgtc gccgtcgatc
      481 tcggcgttga ccgctgggaa ctccttcagc gccacgcagc aggctcggac gaagacgctc
      541 atgaagccca ggcgaacgcc cttgtgcttc ttctcgaagg cgtcgcgata ctggctgcgc
      601 agcgtcatga tcgcggacat gtccacctcg ttgaaggtgg tcagcatcgc cgcgttgttc
      661 tgcgcttcct tcaaccgcag tgcgatcgtg cggcgcaggc gggtcatttt cacccgctgc
      721 tcccgcggct cgtcctggcg cggcgccttc acggccactg gcgccgcttg tgcgttcggc
      781 gctggggcgg caacgcggtt gaggaagtcc atcacatcac ccttggtgat gcggccatcc
      841 tttccggtcc cggtcccgac gtcgctggcc gaaaccctgt tctcggcgat catccttgct
      901 gccgccggca gtggcggatg gaccggcgcg gacgcggccg gtcttgaaac tgggccaagg
      961 gggcgcggcg gcgggttgac cccgggcggc gggttggcgg cgggacgagg ccctggcgtg
     1021 gtagccggct tcgcaatcgg ggctgattta gaagcggcgg ccggcgtgga ggcagcagcc
     1081 ccggccgcat ccagcatacc aagaaccgat ccaacctcga cctcagtgcc ctcgggaacc
     1141 gcgaccgatg acagcacccc ggccgccggt gcgttgacct cgactgtcac cttgtcggtt
     1201 tccagctcaa ccagaggctc gtccgccgcc actgtctcac cttgctgctt catccagcgg
     1261 gcaaccgtcg cggttgttac gctctcgccc aaggcgggca ctacgatttc agtcgccaaa
     1321 gcggtatgtc tccaggcgga cagcgggaat ttaggcgaag cgggaggcgg tctagccgat
     1381 gccgagtacc gtggctacca gccgcgcttg ctgtgccata tgggtacggg cgagaccggt
     1441 ggccgggctc gcggcggcgt ctcggccgac ataggtaggc cggcgaactt tcatgtccag
     1501 gcggagcaac accttctcga tccgccgatc gacgaagttc cacgccccca tgttctccgg
     1561 ctcttcctgg caccagacga cgtcggcgtt ggtataaggc gccagcagac gagttaaggt
     1621 gtttaccggg aacggatata actgctccag ccgcaggatg acaatattct tgatgtcttt
     1681 ggaccgccgt tcctgcagca ggtcatagta aaccttgccg gaacagatca ccacgcggcg
     1741 gatctgctcc gggggattca gttcatcgac ttccgggatg acgaactgaa agcgcgtacc
     1801 ttcggccatg tcggccagcg gcgacacagc cagcttgtgc cgcagcagcg acttcggttc
     1861 gaacacgatc agcggtttgc ggaaattgcg gtgcaactgg cggcgtagcg cgtgaaagta
     1921 gttcgccggg gttgtgggat tgcagacgta catgttccgt tcggcacaca actgcaaata
     1981 ccgctcgatc cgagccgaag aatgctccgg tccctggccc tcgtacccgt gcggcagcag
     2041 cagcacaagg ccggacatgc gcagccactt tgtttcgcca ctggcaagga actggtcgat
     2101 gatgacctgt gcgccgttgg caaaatcgcc gaactgtgcc tcccacaaca ccagcgtgcg
     2161 cggatcggcc agtgagtagc catactcgaa gcccattacg ccggcttccg acagcagcga
     2221 attataaatc tcgatccgcg cctggtcgga gacgatgttg ttcagcggga cgtattcatg
     2281 ctggttcagc tgatcgatca gcaccgcgtg gcgctggctg aacgtgccgc gctgagtgtc
     2341 ctcgccggac agtcgcacgc ggctgccttc cagcagcagg ctgccgaagg ccagggcctc
     2401 gccggtcgcc cagtcgatgc cctcaccggt cgctatcgcc ttctgtttcg cctcaagctg
     2461 gcgagcgatt ttcggattga gattgaagtt ttcaggcaca tgggccagcg ccgcgccgat
     2521 ctgtttcagc gtgtcggcgg gtaccgccgt cgcctcctcg gtgagctcga tttcctgctg
     2581 atcgactggg gtaaagccgc tccaatgtcc ctccagccaa tcggctttat tcggcttgta
     2641 ggacttcgcc gcggaattgg cgtcctccag cgtcttggta aattcgtcca gcatttgccg
     2701 ggactcttcc gcgggaacga cacccgcggc ggccagcttc tcggcgtaca gcgtgcgggt
     2761 ggtcttggtc gcgttgatcg ccttgtacat cagcggctgt gtgaatgccg gctcatccgt
     2821 ctcgttgtgg ccatgacggc ggtaacagac gatgtccagc acgatgtcgc tggcgaactt
     2881 catccggtac tcggcggcca tcctggcgca aaagatcaca gcttccggat cgtcaccgtt
     2941 gacgtgcagg atcggcgcct ggatcgactt cgcgacgtcg gtgcaataca gcccggaata
     3001 cgcgtgcgcc ggcaccgtgg tgaagccaat ctgattgttg acgaccagat ggatcgtccc
     3061 gccggtgcgg tagccgatca actggctcat cgccaaggtt tcatagacca aaccctggcc
     3121 ggcgaaggcg gcatcaccgt gcatcaggat cgccatgacc gatccgcgca tcttggtgtc
     3181 gccggcgcgg tcctggcggg cgcgcacctt gccgaccacc accgggtcga ccgcttccag
     3241 gtgcgagggg ttgggctgta gtgacagatg cacgttgtgg ccggcgatct cgacgtcggt
     3301 ggaggtgccg aggtggtatt tcaca
//
LOCUS       DASWQN010000861         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_214766, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000861 DASWQN010000000
VERSION     DASWQN010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214766"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1489
                     /locus_tag="VGC82_14555"
     CDS             191..1489
                     /locus_tag="VGC82_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424536.1"
                     /translation="MRVQHRHIIYVQGYDPRGLAQYYRMFRTELRKFGRLYQLAATIS
                     RPQVAPDGETASWTIETQASEWQTRTSYDFLRFEDLIQRDLAAPIWRTVLHAMWIYWR
                     LVFGGTIARFWKSHWRFATFITYPHFLLFIEALCALGVAFAFREGLEALGIPGLLSMA
                     AAAALFVATLGTMLKYTENLTYLLYLLSDTIWTWEFSHRQRPEWDERIDRFAQYLVNV
                     ARSSDAEEIVVVGHSSGSFLGTEILGRALKLDPALGRHGPRIVLLTIGGNFPIVGFHA
                     VSQDFRDHLRLLAIEPSIDWIDCQSRKDVMNFYPFDPIAGHGIDVGASRRNPTIVPVR
                     FREIIKPEHYNLFRWKFFRVHFQFVMANERPHAYDFFMIVCGPIPLSERMARPEAALA
                     IATGDAAAREWAWKSLETAVAGCVDAADIGEMEPSARRRG"
     gene            1819..>2258
                     /locus_tag="VGC82_14560"
     CDS             1819..>2258
                     /locus_tag="VGC82_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEY0424537.1"
                     /translation="MQNWNIAVTTFSKTPLLDTIHTPDDLRRLKLEQIRQVADELRQE
                     TIDAVSVTGGHFGAGLGVVELTTALHYVFDTPRDRLVWDVGHQTYPHKILTGRRERMG
                     SLRQLGGLSGFPQRSESEYDTFGTAHSSTSISAALGMAMAAKIKG"
BASE COUNT          422 a          707 c          673 g          456 t
ORIGIN      
        1 caccggcacc gagccgctcg acgttcaatg acggccgggc ggacggaggc gcagtgcccg
       61 tcgctctgcg gcaacagtag agaatattag gcgaattcgg caccacgcgg ccccgatgag
      121 ggctgaaata gctcttggcg cgttacgcgg cctgatatag tccccgggtc agtctgggga
      181 tcagcagtaa gtgcgcgttc agcaccgcca tatcatctat gtgcagggat acgatccgcg
      241 cggcctcgcg cagtattacc gcatgttcag aaccgaactg cgcaaatttg gccggctgta
      301 tcagctcgct gccaccatta gccgcccgca agtcgcaccc gacggcgaga ccgcatcctg
      361 gaccatcgag acccaggcaa gcgagtggca gacccgcacc agttacgatt tcctgcggtt
      421 cgaagacctg atccagcgcg accttgccgc gccgatctgg cgcaccgttc tccatgccat
      481 gtggatctat tggcgtctgg tcttcggcgg caccatcgcg cgattctgga agtcgcattg
      541 gcggttcgcg actttcatca cctacccgca tttcctgctg ttcatcgaag ccctgtgcgc
      601 tctgggcgtc gcctttgcat ttcgggaagg cttggaagcg ctgggcattc ccggcctgtt
      661 gagtatggcc gcggcggcgg ccctgttcgt cgccacactc ggaaccatgc tgaaatacac
      721 ggaaaacctg acctatctgc tttatctgct ctcggacacg atctggacct gggaattctc
      781 tcaccgccaa cggccggaat gggacgagcg gatcgaccgc tttgcccaat acctcgtgaa
      841 tgtcgcacgc agcagcgacg ccgaggaaat cgtggtcgtc ggtcacagtt cgggatcatt
      901 tttggggacc gagatattgg ggcgcgcgct caagctcgat ccggctctcg ggcggcacgg
      961 cccgcgtatc gtcttgctta cgatcggcgg caatttcccc atcgtcggat ttcatgccgt
     1021 gtcgcaggat ttccgcgatc accttcgcct gctggcgatc gagccttcga tcgactggat
     1081 cgactgccag tcgcgcaagg atgtgatgaa tttctacccg ttcgacccca tcgccggtca
     1141 cggcatcgac gtcggcgctt ccagacgcaa tcccacgatc gtgccggtcc ggttccgcga
     1201 aatcatcaag cccgagcatt acaacctgtt ccgctggaag ttctttcgtg tccatttcca
     1261 gttcgtcatg gccaacgagc ggccgcacgc ctacgatttt ttcatgatcg tgtgcgggcc
     1321 gatcccgttg agcgaacgca tggctcgtcc cgaggcggcg ctggctatcg cgaccggcga
     1381 cgccgcggcc cgcgaatggg cctggaaaag cctcgaaaca gcagtcgctg gttgtgtgga
     1441 tgccgccgat ataggtgaga tggaaccatc ggcccgccgc aggggttgag aactgggctt
     1501 gcgccgacct caatcccatc gcgccccgat ggttccggcg ggttggcttt gaccgcagct
     1561 ttagtgtaaa gctctaaggc tgtgggctgt tgagagcgat ctgccgccag ttttcgacgg
     1621 tagtaagcgt ttcaaatagc tcacaaaaat gaccgagaag ggtgaagccg ccaagcgtga
     1681 tgtatatcac attgaacgat ccggagcgca tctaggtttt caaatcggac ttcggcctgc
     1741 tcaggtggca tccggcgtcc gggttttcgc tgcgccgatg ctgcgcaacc catctcaatt
     1801 cgggccgttg gtcccgacat gcaaaattgg aatatcgccg tgaccacatt tagtaaaacg
     1861 ccgcttctcg acacgatcca caccccggac gatctgcgcc ggctcaagct ggaacaaatc
     1921 cggcaagtcg ccgatgagct gcgtcaggag accatcgatg ccgtgtcggt cacgggcgga
     1981 catttcggcg cgggccttgg cgtcgtcgaa ttgacgacgg ccttgcacta cgtgttcgac
     2041 accccgcgcg accgcctggt gtgggacgtg ggccaccaga cctacccgca caagatcctg
     2101 accggccggc gcgagcgcat gggcagcctg cgccagctgg gcggcctgtc gggctttccg
     2161 cagcgcagcg agagtgaata cgacaccttc ggcaccgccc actcgtccac cagcatctcc
     2221 gcggccctgg gcatggcgat ggcggcgaaa atcaaggg
//
LOCUS       DASWQN010000862         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1930312, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000862 DASWQN010000000
VERSION     DASWQN010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1930312"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VGC82_14565"
     CDS             complement(<1..449)
                     /locus_tag="VGC82_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0424538.1"
                     /translation="MEEVDCVVAGAGVVGLAVARALALAGREVIILDAAESIGTETSS
                     RNSEVIHAGIYYPAGSLMARFCVAGRRMLYPYCAEKGVPHVNCGKLIVATNAQEDAML
                     AGIKLRAEANGVEGMRVLSAAEAIALEPNLACTSALLSPATGIIDSHS"
     gene            514..1380
                     /locus_tag="VGC82_14570"
     CDS             514..1380
                     /locus_tag="VGC82_14570"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEY0424539.1"
                     /translation="MLSLNAGASSIVVAPELGGGLTGWIVGRTPVLRRALPQATVGGD
                     RHALGCFPLLPYGNRIGGARFRWSGVDYALQRNFGDAPHTIHGLGWQRGWTVETSTTR
                     SVTLRLRHDPDPTWPFAFDAEVGYNLSETALTVTIRITSRHPVPAPAGIGVHPFFPKV
                     DAASLRFDATGVWENDAGALPLRHGPPQPGWLHRRPRFIRDSRLDNCFTGWNGSADIQ
                     AGPAGLRIEASFVFRQLQVFTPSWADFFCVEPVSHVPDAVNRPDLPPEQAMHILQPND
                     TLSGTVSFILTG"
BASE COUNT          255 a          592 c          448 g          250 t
ORIGIN      
        1 gaatggctgt cgatgatccc cgtcgccggc gacagcagcg cgctggtgca ggccaggttt
       61 ggctccagcg cgatcgcctc ggcggcactc agcacccgca tgccctcgac cccattggcc
      121 tccgcccgca gcttgatgcc cgccagcatc gcatcttcct gcgcgttcgt cgccacgatc
      181 agcttgccac aattcacatg cggcacaccc ttctccgcgc agtacggata cagcatgcgc
      241 cgcccggcca cacagaaccg agccatcagt gacccggcgg gatagtagat cccggcatgg
      301 atcacctcgc tgttgcgcga cgacgtctcg gtgccgatgc tttcggcggc gtccaaaatg
      361 atgacctcac gcccggccag cgccagcgcg cgcgccaccg cgagacccac cacgccggca
      421 ccggccacga cgcaatccac ttcctccacg cctgattctc ctcaccgaaa cgctggccga
      481 cgggttaggc tgggcgcgca gcggagtcca tacatgctca gcctgaacgc gggcgcgtca
      541 tcgattgttg tcgcaccgga gctcgggggc ggcctgaccg gatggatcgt tggccgcacg
      601 ccggtgctgc ggcgggcgct gccccaggcg acagtcggcg gcgaccgcca tgcactggga
      661 tgtttcccgc tgctccccta cggcaaccgg atcggcggcg cccgctttcg ctggtccggc
      721 gtcgactacg cattgcagcg caacttcggc gacgcaccgc acacgatcca cggcctgggc
      781 tggcagcggg gctggaccgt cgaaacatcc accacacggt cggtcacact gcggctgcgc
      841 cacgaccccg atccaacatg gccgttcgcc ttcgacgccg aggtcggtta caacctgtcc
      901 gaaaccgccc tgaccgtgac gatcaggata actagccgcc atcccgtccc ggccccggcg
      961 ggcatcggcg tgcatccgtt ctttcccaag gtcgacgctg cctcactccg cttcgacgcc
     1021 accggcgtgt gggagaacga tgccggcgcg ttgcccctgc gccacggtcc gccacagccc
     1081 ggctggctgc atcgccggcc acgcttcatc cgcgactcgc ggctcgacaa ctgtttcacc
     1141 ggatggaacg gatcggcgga catccaggcc ggacccgccg gcttgcggat cgaggccagc
     1201 tttgtgttcc gccagcttca ggtgttcacc ccatcctggg ccgacttctt ctgcgtcgaa
     1261 ccggtcagtc acgtgcccga cgcggtgaac cggcccgacc tgccgccgga acaggcgatg
     1321 cacatcctgc agccgaacga cactctcagc ggcaccgtca gcttcatcct gaccggctga
     1381 tcaaccggcc tttgccatca ccgcgccttt gccatcatcg ccagtgccgc ctcgggccct
     1441 gccagaagct ccacaagacg aatctgatca accagcgcca gatgaccgcc cgccgcgctc
     1501 cctttgatca cctccgacgc gcgccgcagc aaaagatcgt acgtc
//
LOCUS       DASWQN010000863         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_610732, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000863 DASWQN010000000
VERSION     DASWQN010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610732"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="VGC82_14575"
     CDS             complement(<1..1503)
                     /locus_tag="VGC82_14575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0424540.1"
                     /translation="MTDADHEAFARRWAEAIVGTSYVSMGRRVLAKHLLGLTSQLVDA
                     ALAAEFDPAIGRQVGVDMVAAHFTGTETLRETLALIAEGLPGLLGSAPGCADTGSRVA
                     QLAASMAAGYAGALRERSLDEQDAVYRAGLRARRQAEQALAASEAKFRAMFTDAAIGI
                     GIGDLEGNITDANPALQQMFGYGHEEFTRLHLSAMVHPDDAASVWQTYEELIRGERDH
                     FRLEKRYYRSDGGEIWTHVTVSLVRDESGEPAYQVAMLEDISERRSLEAKLHHQAYHD
                     PLTALANRQMVTERLTQIFAGPAEQRRVGLCFLDLDGFKVVNDSLGHHVGDQLLKTVA
                     SRLTRCCGPGQLVARMGGDEFVIIAEGTAGEEDVAALADKVLAAMAAPIRVGKHELTV
                     TTSIGVVERPVADTNPEDLLQAADITMYRAKANGKARYAMFDQNRNDNEVARFTLSAT
                     MLAAVERGEFFVEYQPLLRLRDRSLIGVEALVRWRHPTFGVLGPERFIDLA"
BASE COUNT          283 a          540 c          454 g          297 t
ORIGIN      
        1 cgccagatcg atgaagcgtt caggtcccag caccccgaat gtcggatgac gccaccgtac
       61 cagtgcctca acgccgatta gcgaacgatc ccgtagccga agcagcggct ggtactcgac
      121 gaagaactca ccccgctcca ccgcggcaag catcgttgcg gacaacgtga aacgagccac
      181 ttcgttatca ttacggttct ggtcaaacat cgcgtagcga gccttgccgt ttgctttcgc
      241 tcggtacatg gtgatatcgg cggcttgcag aaggtcctcg ggattggtgt cggcgactgg
      301 tcgctcaacc accccgatgc tcgtcgtcac cgtcagctca tgcttgccga ctcgaatcgg
      361 cgcggccatc gcggccagca ccttgtccgc cagcgccgcc acgtcttcct ctccggcggt
      421 accctcggcg ataatcacga attcgtcgcc gcccattcgg gccaccagtt gccccggtcc
      481 gcagcagcgg gtgagccggg atgcgacggt tttcagcaac tggtcgccga cgtggtgtcc
      541 caagctgtca ttgaccactt tgaacccgtc caggtccagg aaacacagcc caacccgacg
      601 ctgctcggca ggcccggcga atatctgcgt caggcgttcg gtgaccatct gccggttcgc
      661 cagcgccgtc aacgggtcgt ggtaggcctg gtgatgcagt ttggcctcca ggctccggcg
      721 ttcggagatg tcctccagca tcgccacctg gtaagccggt tctcccgact cgtcgcgcac
      781 caacgacaca gtcacgtggg tccagatctc cccgccgtcg gatcggtagt accgtttttc
      841 caggcggaaa tggtcacgct caccccgaat aagctcttcg taggtttgcc acacgctggc
      901 cgcgtcatcc ggatggacca tcgcactgag gtgcagcctg gtgaactcct catggccata
      961 accgaacatc tgctgcaatg ccggattggc atcggtgata ttgccctcta aatcaccgat
     1021 gccaattcct atcgccgcgt cagtgaacat ggctctgaat ttcgcctcgc tggccgcgag
     1081 tgcctgctcg gcttgcctgc gcgcacgcag accggcgcga taaaccgcat cttgttcatc
     1141 aaggctgcgc tcacgcaatg ccccggcgta tccggctgcc atgctggcgg ccagctgagc
     1201 gacccggctg ccggtatcgg cacatcctgg agccgaaccc aacagacccg gcaaaccctc
     1261 ggcgatcagg gctagggtct ctctgagcgt ctcggtgccg gtgaaatgcg ccgccaccat
     1321 gtcgacgccg acctggcgcc cgattgccgg gtcgaactcc gcggccagcg ctgcatcgac
     1381 caactggctg gtgagcccaa gcaaatgttt ggcgagcacg cggcgtccca tcgacacata
     1441 gctcgtgccg acaatcgcct cggcccagcg ccgggcgaac gcctcgtgat cggcgtcggt
     1501 catgcccgcc ggcctactac cgcatagccg gtgaaacgct cgggatgatc atcgacatcg
     1561 gccggcgagt ccgg
//
LOCUS       DASWQN010000864         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2062625, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000864 DASWQN010000000
VERSION     DASWQN010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062625"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1089
                     /locus_tag="VGC82_14580"
     CDS             217..1089
                     /locus_tag="VGC82_14580"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0424541.1"
                     /translation="MLTKAQIEEYNDVGAIVVPDVLTPDEVRTLSDVTDGFVERARNL
                     TGHTDIYDLEDSHTADNPRVRRIKTAHLHDPAYAALVRHPKIIAVLQALWGPDIRFDT
                     AKLNMKCAGFGAPVEWHQDWAFYPHTNDNLAAVGVMFDDMAMENGPLMIIPGSHKGPT
                     FDHHADGVFCGAMDPANRDVDYGKAIALTGKAGSITVHHVRAVHGSAPNVSDRDRRLL
                     LFQFRAADAWPILGFPAGIEKFDALMVAGQSRQPRLSPAPVRLPLPPALLQGSLYENQ
                     KGMKNRFFDMVAAQ"
     gene            1086..>1681
                     /locus_tag="VGC82_14585"
     CDS             1086..>1681
                     /locus_tag="VGC82_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424542.1"
                     /translation="MSQSSGSQSPGSQSPGSRPPAMEPIFDRTPHPFGGGPVDPVVYD
                     ASVAALRQRTVNFAARRYADMMMVGGLDRATADRHFRLRAAVVPAGLDARPIGLTIQS
                     EAAAAHAAGDFNIETDAADSRVTVRWGDRVGHAVSRPGYGSILLRPGIAPGFDPQPIS
                     RHFPHAGRPWPIGDAVETGAAPPATFADASDAFFAGSPG"
BASE COUNT          280 a          579 c          540 g          282 t
ORIGIN      
        1 gtccggcgat ggtgcgaatc gtatgccccg attctagcgc ccgtttccgc gtcgcgttaa
       61 gtccgacagg ctgctagagt ggaagccgtg cgcccatcag cacggatttc actctaacca
      121 cgggaattcg cctctgctcc cggggacttg gccggcccgg ctgtagcctc ttcgcggaaa
      181 gtcggtgtag cctctgatcc aagaacggag ggatcgatgc tcaccaaggc tcagatcgag
      241 gaatacaacg acgtcggcgc catcgtcgtc ccggatgtgc tgacaccgga cgaagtacgg
      301 acgctcagcg acgtgaccga cggtttcgtc gagcgcgccc ggaatctgac aggccacacc
      361 gacatctacg acctggaaga cagccacacc gcggacaacc cgcgggttcg ccgcatcaag
      421 accgcccatc tgcacgaccc ggcctatgcc gccctggtcc ggcatccgaa aatcatcgcc
      481 gtgctgcagg cactgtgggg tccggacatc cgtttcgaca ccgcgaagct gaacatgaaa
      541 tgcgccgggt tcggtgcgcc ggtggaatgg caccaggatt gggcgttcta tccgcacacc
      601 aacgacaacc tcgccgcggt gggcgtcatg ttcgacgaca tggcgatgga aaacggaccg
      661 ctgatgatca ttcccggcag ccataaggga ccgaccttcg atcaccatgc cgacggcgtt
      721 ttctgcggcg caatggaccc ggccaaccgc gacgtggact acggcaaagc catcgcgctg
      781 actggcaagg ccggctcgat caccgtgcat catgtccggg cggtgcatgg atcggcgccg
      841 aatgtgtcgg accgggaccg gcggttgctg ctgttccagt tccgagcggc cgacgcctgg
      901 ccgatcctcg gctttcccgc cggcatcgag aaattcgacg cgctgatggt tgcgggccag
      961 tcgcgccaac ccaggctctc gcccgcgccg gtgcgtctgc cgctgccgcc agccctgctg
     1021 cagggctcgt tgtatgagaa ccagaagggc atgaagaacc gcttcttcga catggtggca
     1081 gcgcaatgag ccaatcctcg gggagccagt ccccgggcag ccagtccccg gggagccggc
     1141 cgccggcgat ggagccgatc ttcgaccgca cgccgcatcc gttcggcggc gggccggtcg
     1201 atccggtggt ctatgacgcc tcggtcgccg ccttgcgcca gcgcacggtc aatttcgccg
     1261 ctcggcgtta tgccgacatg atgatggtcg gcggcctcga tcgagccacg gccgatcgtc
     1321 attttcggct gcgcgcggcc gtggttccgg cggggctgga cgcccgcccg atcgggttga
     1381 ctatccagtc ggaagccgcc gccgcccacg ccgccggtga cttcaacatc gagacggacg
     1441 cggcggacag ccgcgtgacc gtgcgctggg gggatcgcgt cggccatgcc gtctcccgtc
     1501 ccggctacgg cagcatcctg ctgcgccccg gcatcgcacc cgggttcgac ccgcagccca
     1561 tcagtcgtca cttcccgcat gccggtcggc cctggccgat cggagatgcg gtggaaacag
     1621 gcgcggcccc acccgctacg tttgccgacg cgtcggacgc attcttcgcc ggttcgcccg
     1681 g
//
LOCUS       DASWQN010000865         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_875255, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000865 DASWQN010000000
VERSION     DASWQN010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875255"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..856)
                     /locus_tag="VGC82_14590"
     CDS             complement(14..856)
                     /locus_tag="VGC82_14590"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0424543.1"
                     /translation="MTVNAATAIARAGNETEDVLILRREGIVGQKAAFSREWVEQLRE
                     DMMTAFWDAIQRPGGAISRGPRRWYVELHPQALSGFRDLVTHPWITAMAEGILGADYE
                     IVEVGFDTPFQGAKNQPWHRDFPSPQATYEHRRITSLAFNLTGVDVTPDMGPFEVAPG
                     TQWDDGRSWKHEMFPDRAAWPGYEERGIRKYPQMGDISCRSALTMHRGTAHPSPVARP
                     VLVIGIDAPGAGHAALHDMMMTKDYHAALRRSLQDRIKCRVVDKLIPITQKHDIEELV
                     MGTE"
     gene            complement(945..2014)
                     /locus_tag="VGC82_14595"
     CDS             complement(join(945..1525,1525..2014))
                     /locus_tag="VGC82_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611116.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY0424544.1"
                     /translation="MSAAVKITCTDHLPAELRGVAAKSDDAATARRLLAIAMVLEGTS
                     RLDAARRTGMDRQTLRDWVIRYNERGLDGLASRKPPGAAPKLTAAQMSELSDLVLAGP
                     DPKVDKVVRWRCLDLRAEVTRRFSVTVPERTIAKWLCKLKLTRLQPRPFHPKKDAAAQ
                     EAFKKNFSALLKQALLGSTAATPIEIWFQDEARVGQKGGHTYVWAKVGSRPPMIRDNR
                     HDSAYIFGAICPDRAVGAAIIAPAANTECMNLHLLEISTQVAPGAIAGLVCDSAGWHQ
                     TGGEPTVPDNIVLLPLPPYSPELNPMENVWEYLRGNKLCATVWDSYEAIVEACADAWN
                     WFVNDPDRIRSIGTRGWATVNV"
     gene            complement(2057..>2278)
                     /locus_tag="VGC82_14600"
     CDS             complement(2057..>2278)
                     /locus_tag="VGC82_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424545.1"
                     /translation="SRLLDVSKLNADDKAALDNTTKARGEVLAKWSSLGLKESAPLWY
                     YVLREAEYYGVIPAAPDADSPSILKRCEM"
BASE COUNT          409 a          719 c          667 g          483 t
ORIGIN      
        1 cgatcttgcc gcgtcattct gttcccatca ccaactcctc gatatcgtgc ttttgcgtaa
       61 tggggatcaa cttgtcgacg acgcgacatt ttattctatc ttgtaacgat cgccgcagcg
      121 cggcatggta atccttggtc atcatcatgt catgcagcgc cgcatgtccc gctccgggtg
      181 catcgatacc gatgacaagc accgggcgcg cgaccggcga cggatgtgcc gttccacgat
      241 gcatggtcag agccgaacgg cacgagatat cacccatctg cggatatttg cggatgcctc
      301 gctcttcgta accgggccag gcagcgcggt ctggaaacat ttcgtgcttc catgaccggc
      361 cgtcgtccca ttgtgtgcca ggcgcgactt cgaaaggccc catatccggg gtcacgtcca
      421 cgccggtcag gttgaacgcg agcgaggtga tccggcgatg ctcgtatgtc gcctgtggcg
      481 acgggaagtc gcggtgccag ggctggttct tcgctccctg gaacggcgtg tcgaagccaa
      541 cctcaacgat ttcgtagtcc gctccgagta tgccttccgc catggctgtg atccaaggat
      601 gggtcaccag atcgcggaac ccgctcagcg cctgcgggtg caactccaca taccatcgtc
      661 gcggccctcg gctgatggca ccgccgggac gttgtatcgc gtcccaaaag gccgtcatca
      721 tatcttcccg gagttgctcg acccattcgc gggaaaacgc agccttctgg cccacgatgc
      781 cctcacgccg caggatcaga acgtcttcag tctcgttacc agcgcgggcg atggctgttg
      841 cagcattcac tgtcatcggt atcgtcttcc cttatgacct ggccggtaca gtcaggccgc
      901 atgattcccg ccggtcagat ttggaaagcg ttataccagc ggccctagac attgaccgtt
      961 gcccatcccc ttgttccgat cgagcggatg cggtctggat cgttaacgaa ccaattccag
     1021 gcatccgcgc aggcttcgac gattgcctcg tagctgtccc agaccgttgc gcagagcttg
     1081 ttgccgcgaa ggtattccca gacgttttcc atcggattca gttccggcga atagggcggg
     1141 aggggcagca gaacgatgtt gtctggcact gtgggttcac cgcctgtctg gtgccaaccg
     1201 gcactgtcgc agaccagccc ggcgatcgcg cctggggcaa cctgtgtgct gatctccaga
     1261 aggtgcaggt tcatgcattc ggtgttggcg gcgggggcga tgatcgccgc gccgaccgcg
     1321 cggtcgggac agatggcgcc gaagatgtag gcactgtcgt gccggttgtc gcgtatcatc
     1381 ggcgggcgcg agccgacctt agcccagaca taggtgtgcc cgcctttctg gccgacccga
     1441 gcctcatcct gaaaccagat ttctatcggt gtcgccgcgg tgctaccgag caaggcttgt
     1501 tttagcaggg cactgaagtt tttttaaaag cctcctgcgc cgctgcatcc tttttaggat
     1561 gaaacggacg cggttgcagc cgggtcagtt tcagtttgca cagccacttg gcgatggtgc
     1621 gctccggcac cgtgacgctg aaacgccgag tcacctccgc acgcagatcc aggcaccgcc
     1681 agcgcaccac cttgtccact ttggggtcag gtccggcgag cacgagatcg ctcagttcgc
     1741 tcatctgtgc cgcggtcaac ttcggcgccg caccaggcgg cttgcgcgat gccaggccgt
     1801 caaggccgcg ctcgttatag cgaattaccc agtcacgcag ggtctgccgg tccatgccag
     1861 tccgccgggc agcatcaagt cgggaggttc cctccagcac cattgcgatc gccagaagcc
     1921 gacgggccgt cgccgcgtca tcactcttcg ccgctacccc gcgaagctcc gctggcaggt
     1981 gatccgtgca ggtaattttg acggctgcgc tcatccgatc ctccaggcgg cgaatcgccg
     2041 cgtcggatgc agcgaatcac atttcgcacc gtttgagaat cgagggagag tcggcgtcag
     2101 gggcggctgg tataactccg tagtactcgg cctcacgcaa gacatagtac cagagcggag
     2161 cgctctcctt gagccccaaa ctgctccact tcgccagaac ctcacctctg gccttggttg
     2221 tgttatccaa ggccgcctta tcatcggcat tcagcttgga tacatccaag agccgtga
//
LOCUS       DASWQN010000866         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1798788, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000866 DASWQN010000000
VERSION     DASWQN010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798788"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..84
                     /locus_tag="VGC82_14605"
     CDS             <1..84
                     /locus_tag="VGC82_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008854809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0424546.1"
                     /translation="SVEIPEDAAEKIATVKDAIDYIEKQKQ"
     gene            118..1380
                     /gene="fabF"
                     /locus_tag="VGC82_14610"
     CDS             118..1380
                     /gene="fabF"
                     /locus_tag="VGC82_14610"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY0424547.1"
                     /translation="MRRVVVTGMGIACPLGVGVEHVWRRLINAESGIGPITAFDASDL
                     PCRVAGQVPPGNRASGGLDINEWIPIRDQKKMDRFIHLAMVAGAEAVEDSGWVPRTED
                     DYCATGVMIGSGIGGLETIYHASILVHEGKARRLSPFFIPSALGNLASGHLSIKYGFK
                     GPNHSVVTACATGVHAIGDASRLIALGDADVMLAGGSEATVCAIGIAGFCASRALSTG
                     FNDRPAAASRPWDKDRDGFVMGEGSGCLVLEEYEHAKKRGAKIYAEVSGYGMSGDAHH
                     ITSPAEGHDGAYRSMRAAMRNGGFSPADIQYVNAHGTSTPMGDDMELEAVERMFGNSA
                     RGLAMSSTKSATGHLLGAAGAIEAVFSILAIRDGVAPPTLNLDEPSRQSVIDRVVKTA
                     QQRRIKVVLSNSFGFGGTNASIIFRAAA"
     gene            1428..2393
                     /gene="mltG"
                     /locus_tag="VGC82_14615"
     CDS             1428..2393
                     /gene="mltG"
                     /locus_tag="VGC82_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY0424548.1"
                     /translation="MATIAVVGAMAAGGFQWWNEALRQPGPLAEARAIVIPRGGARQL
                     ADALESNGVISQTMIFRAAAWVTKGDGTLHAAEFAFPAHASVRQVLAILRAAHPVEHH
                     LAIPEGLTARQIAVVLAHAEAMTGEIDAISEGSVLPETYAYEYGADRTAIVARAEAAM
                     DKELAAAWADRAPDLPLASPREALILASIVERETAKPDERAHVAAVYLNRLRQGMKLQ
                     ADPTVAYSASGGTGVLDHRLTRAELGRDDPFNTYRSAGLPPAPICSPGLGSLLAVMHP
                     AESDDLFFVADGTGKHVFSRSYQAHDAAVARWRAMGGSAPHETPD"
BASE COUNT          488 a          799 c          895 g          525 t
ORIGIN      
        1 agcgtggaaa ttcccgagga tgctgccgaa aagatcgcta ccgtcaagga tgcgatcgac
       61 tacatagaaa agcagaagca gtaagttttt cctgattagc acgaaaggcc tgcggtaatg
      121 cggcgtgtag tggtgacggg catggggatc gcctgtccac tcggcgtggg cgtggaacat
      181 gtctggcgtc gtttgatcaa tgccgaatcc ggtattgggc cgatcacggc gttcgatgcc
      241 tcggatctgc cttgcagggt tgccggacag gttccgcccg gcaaccgggc ttcgggcggc
      301 cttgacatca atgagtggat accgatcagg gatcagaaaa agatggatcg ctttatccat
      361 ctggccatgg tcgccggtgc cgaggcggtc gaagattcgg gctgggtacc gcgaaccgag
      421 gacgattact gcgcgaccgg cgtgatgatc ggttcgggta tcggcggcct ggaaacgatc
      481 tatcacgcct cgatccttgt ccacgaaggc aaagcgcggc ggttgtcgcc gttctttatt
      541 ccttcagccc tgggcaacct tgcctcgggt catttgtcga tcaaatacgg ctttaaaggc
      601 cctaatcatt ccgtcgtgac ggcatgtgcc actggcgtgc acgcgatcgg cgacgcatcg
      661 cgcctgattg cgcttggcga tgccgatgtc atgctagcgg gcggttcgga ggccacggtg
      721 tgtgccatcg gcatcgccgg attctgcgcc tcccgcgcgc tgtccacggg gttcaatgac
      781 cgtccggccg cggcgtcgcg tccgtgggat aaggatcgtg atggctttgt catgggcgaa
      841 ggctccggct gcctggtgct ggaggaatac gagcacgcca agaagcgtgg cgcaaagatt
      901 tacgcggagg tgtccggcta cggcatgtcg ggtgatgccc atcatatcac gtcaccggcc
      961 gaggggcacg acggcgcata ccgctcgatg cgggcggcga tgcgcaacgg cgggttcagc
     1021 ccggcggata tccagtacgt caacgcgcac ggcacctcga cgccgatggg cgacgatatg
     1081 gaactggaag ccgtcgagcg gatgttcggt aattcggccc gtggcttggc catgtcgtcc
     1141 accaagtcgg cgaccggaca cttgctgggt gcggccggtg cgatcgaggc ggtgttttca
     1201 attctcgcga tccgggacgg tgtggcgccc ccgacgttga acctggatga accgagccgg
     1261 cagagcgtca tcgatcgggt ggtgaaaacg gcacagcagc ggcggattaa agtggtcctt
     1321 tccaacagct tcggcttcgg cggcaccaac gcctcgatca tcttccgcgc tgctgcatga
     1381 ttggccaccg ccccctgggg cggtggctcc ggcggacact agctgggttg gcaacgattg
     1441 ccgtggtggg cgccatggcc gccggcgggt ttcagtggtg gaacgaggcg ctgcggcaac
     1501 ccggcccact cgccgaggcg cgggccatcg tcatcccgcg gggtggcgct cgtcagcttg
     1561 ccgacgcact ggagtccaac ggggtcatca gccagaccat gattttccgt gccgccgcct
     1621 gggtcaccaa gggggacggc acgctacatg ccgcggaatt cgcctttccc gcccatgcgt
     1681 cggttcgcca ggtccttgct atcctgcgcg cggcgcatcc ggtcgagcac cacttggcca
     1741 tccccgaggg gctgaccgcc cggcagatcg cggtcgtgct ggcacatgcc gaagcgatga
     1801 cgggggaaat cgatgcgatt tctgaagggt ctgtccttcc ggaaacctac gcctatgaat
     1861 atggcgccga ccgaaccgcc atcgtcgcac gggccgaagc cgcgatggat aaggaactgg
     1921 ccgccgcttg ggcagaccgg gcgccggacc taccgttggc ctcccctcgg gaggcgttga
     1981 tccttgccag catcgtggaa agggagacgg caaaacccga cgagcgcgcg catgtcgcgg
     2041 cggtgtacct gaaccggttg cggcagggca tgaagctgca agccgacccg acggtagcct
     2101 actccgccag cggtggcact ggcgtgctgg atcatcgcct gacccgcgcg gagcttggtc
     2161 gggacgatcc cttcaatacc taccgcagtg ccggtctgcc gccggccccg atttgctcgc
     2221 ctgggttggg cagcttgcta gccgtgatgc atccggcgga aagcgacgat ttgttcttcg
     2281 tggctgatgg caccggcaag catgtcttct cccgcagtta tcaggcccac gatgcggctg
     2341 tcgcgcgttg gcgggcgatg ggcggctctg ctccgcatga gactccggat tagggcatga
     2401 tatcgtcgac cgggacgttc gcgccatccg tttcgcagtg ctgcctgtga agcgtcgcag
     2461 taacgcgaag gcacaatcgc taacgaagtc ccggagggtt ttgactttcg tcaggcaccg
     2521 agctggaagt accggaactg caattggatc gcttgcacga tgcggtcaac attgtaaact
     2581 tatttgacat cacagcagcg gactcgaggg ccatcacttt gaacgagacc gcaaatctat
     2641 ccaagccttt ggcggccaac gatcttgttt cagagatatg ggaacggacg ttcggcatac
     2701 cggcaga
//
LOCUS       DASWQN010000867         5721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1534149, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000867 DASWQN010000000
VERSION     DASWQN010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534149"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..1585
                     /locus_tag="VGC82_14620"
     CDS             326..1585
                     /locus_tag="VGC82_14620"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424549.1"
                     /translation="MDTKPSKVLGMPTGFGGMRGQFGLNGLNFFAAAVQAGFGPFIAV
                     WLAQQGWSLNAFGLVLSIGTLTALLGQLPGGMLVDHIHAKRYAAAAALAVLACSAISL
                     CLSSSFPVVAAAEMAHGLASCVLTPAIAALTLSVCGHENFAERLGLNARYAALGNAAS
                     AAALGAASFYVSQRAVFLLTAVLVVPAMASLLMIRASAPTEDDHPALKHPKQREHPAW
                     RIFAEPALHVFAVAVVLFQLANAALLPLALNGLARLHDAPGFLVSATIVVPQIITAAL
                     APWAGRLAQRIGRRPVLIVGFAAIPVRALSFATVQGALPLAVFQALDGISAAVFGLML
                     ALIAADLTRRTGYLNLAIGALGLASGLGATFSTVLAGWLADRFGAQATFLFLAGVGAA
                     ATCLLWLAMPETRPARTRHGRKAAHPA"
     gene            1666..2784
                     /locus_tag="VGC82_14625"
     CDS             1666..2784
                     /locus_tag="VGC82_14625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY0424550.1"
                     /translation="MDQAEAKHLFEPTGDFQCLHEFLPAARAKLSDHIWGYLVGAAET
                     ETTLRRNRLAIDSIALRPRVCVDVSKIDLGTTLFGRKLRMPVILAPVGSLESFHPGGA
                     ATAGRAASAFGIPIMVSSVTHPGLEEAAAAATGPKIFQLYVRGDDAWIDDVVKRAVAA
                     GYDAFAITVDTAAYSRRERDIAGRFVKPWRAAATGQNYQSGFTWDNVKHFKDKHGIPL
                     ILKGIATGEDAALCCEHGVDGVYVSNHGGRQLDHGRGSMDVLPEVMAAVRGRAKVIVD
                     GSICRGTDIVKAIALGADAVAMGRMYCYALAADGEAGVHKMLELLEHELGVAMALAGA
                     RGLGELHPGYLFRNAPQVTPTHAHSAFPLLQATPEYRG"
     gene            complement(2980..3630)
                     /locus_tag="VGC82_14630"
     CDS             complement(2980..3630)
                     /locus_tag="VGC82_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0424551.1"
                     /translation="MSETSNRIVWGRNTSSNVMKVLWGLGELGLAFERIDVGGAFGKT
                     DTPEYLGMNPTGLVPTLQEGPFTLWESNAILRYLAQTHASGSPLWPTEPHARANIDRW
                     MDAQQTVLNRPMSGVFWGLVRTAPDKRDLKALEASIAETARAWRMIETGLKQHAFIAG
                     SDFTLCDIPWGVHAHRWFNMDYLGLTRPDIPALRAWYDRLCERPAYRQHVMATPVV"
     gene            3744..4253
                     /locus_tag="VGC82_14635"
     CDS             3744..4253
                     /locus_tag="VGC82_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY0424552.1"
                     /translation="MQGRPPRNLAVLAALGAAAALGVAFAAETWGELVPCALCLLERW
                     PYRVVIVLGLLAAVVPGGIARMLLMLVIVCLLADAAVAAVHVGVEMGWWPSPLPECAA
                     PRFSGGSIADRLASMPSRPAKPCDEPTFLVPGFPVSFAAMNLLFAMGFAALLAMSLSG
                     SILPRRNRR"
     gene            4250..4789
                     /locus_tag="VGC82_14640"
     CDS             4250..4789
                     /locus_tag="VGC82_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEY0424553.1"
                     /translation="MTDELPGDLSAQEKTKRMIRVDHAGEYGATRIYAGQLALLGRGG
                     KGDLLRHMQAQEQAHLHTFNGMIADRRVRPTALLPLWHLAGFALGAVSAAMGEKAAMA
                     CTVAVEEAIDEHYSAQLESLGAAEPELRQIVEKFRSEELEHRDLGLDHGAEQAPGYQL
                     MSRVIKAGCRAAIAISERI"
     gene            4963..5457
                     /locus_tag="VGC82_14645"
     CDS             4963..5457
                     /locus_tag="VGC82_14645"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEY0424554.1"
                     /translation="MIAALAWALPVRAQPKLPPGLLEYAASALARLIDDARQQAIADG
                     VRPIQATTYRALLGYFPPALLQRCRFATGTSRTLTMPALAFSYGDASAITLGDVVLFK
                     SERVADSDLKVWAHELTHVMQYQRWGVDGFAERYVRDSAAVEREATDNANRFVTWHAT
                     QAGR"
BASE COUNT          949 a         1787 c         1860 g         1125 t
ORIGIN      
        1 catatacata actaatcaca acttggtgac acttcctcat gaccgctcag ggacaatcgg
       61 ttcgcgggac ggtctccagc aatgaaacga cttcgatatt gtcttcaggc gcgcagcgac
      121 agcttgcgcg caggcatcat cgacctcgag cagcatgatg tcggtatccc ggcgatccgc
      181 gcgccaacgc cggccgggca tgatcggcgc cgaatctggt tgacgacgcc gccccagcgt
      241 gcggtcgctt gatcgagcgc gacatgccaa cgatgctccc atcgcccgcc gtgacgtttg
      301 cggcgagttg gtcctgaaat agaatttgga caccaaaccg agcaaagtgc tcggcatgcc
      361 gacggggttt ggcgggatgc gcggccagtt cggcctgaac ggcctgaatt tctttgctgc
      421 cgcggtccag gccgggttcg gcccatttat cgccgtctgg ctcgctcaac aaggctggag
      481 cctcaacgcg tttggtctcg tgctcagcat cggcacgctt accgccctgc tcggacagtt
      541 acccggcggc atgctcgttg accatattca tgccaagcgc tacgccgccg cggcggccct
      601 ggcggtcctc gcctgttccg ccatttcgct gtgcctgtcg tcatcctttc ccgttgtcgc
      661 ggcggccgag atggcccacg gcctcgcaag ctgcgtgctg acgccggcga tcgcagctct
      721 gacgctgtcg gtatgtgggc atgagaattt tgccgaacgg ctcggcctga atgcgcgcta
      781 tgccgcgctc ggcaatgccg cttcggccgc tgccttgggc gctgcctctt tctacgtctc
      841 ccagcgtgcg gtttttctgc tgaccgcggt gctggtggtg cccgccatgg cctcgctgct
      901 catgatccgc gcctccgctc caaccgagga cgaccatccg gcactgaagc acccgaaaca
      961 gcgggagcat ccggcctggc ggatctttgc cgaaccggcg ttgcatgttt tcgctgttgc
     1021 ggtcgtgctg tttcaactcg ccaatgcggc attgctgccg ctggcgctga acgggctggc
     1081 gcgcctgcac gatgcgcccg gatttctggt atccgcgacg atcgtggtgc cgcaaatcat
     1141 caccgccgcc ctggcgcctt gggcaggcag acttgcacaa cgaatcggcc gccggccggt
     1201 gctgatcgtg ggcttcgcgg cgatcccagt ccgagcctta tcgttcgcca cagtgcaagg
     1261 cgcgctgcca ctggccgttt tccaggcgct ggatgggatc agcgcggcgg tgttcgggtt
     1321 aatgctggcc ctgattgccg ccgacctgac ccggcgaacc ggctacctga acctggcaat
     1381 cggcgcactc ggtttggcgt ccggcctagg ggccaccttc agcaccgtcc ttgccggctg
     1441 gctcgccgac cgcttcggcg cccaggcgac attcctgttt ctggccggag tcggcgccgc
     1501 ggcgacttgc ctgttatggc tcgccatgcc ggaaacgcgc ccggcgcgaa cgcggcatgg
     1561 gcgcaaggcc gcgcatccgg cctgaccggc tcttgcgcgt tcgcctgctc caaggcctga
     1621 taccggctag gtcgccaacg tagatcatgt tttgaggggg aaaccatgga ccaagctgaa
     1681 gccaaacatc ttttcgagcc aacgggcgat tttcagtgcc tgcatgaatt tctccccgcc
     1741 gcccgcgcca aattgtccga ccatatctgg ggctacctgg tcggcgccgc ggaaaccgag
     1801 accacgctgc ggcgcaaccg cctcgccatc gactcgatcg cgctgcgccc gcgcgtttgc
     1861 gtggacgtgt cgaagatcga cctcggaacc accttgtttg gccgtaagct taggatgccg
     1921 gttatcctcg cccccgtggg ttcgttggag tcatttcatc ccggcggcgc cgccacggcg
     1981 ggacgggctg cctccgcctt cgggatcccg atcatggtga gttcggtgac ccatcccggc
     2041 ctggaggaag ccgcggcggc agcgacggga ccaaagattt tccagctata cgtccgaggc
     2101 gacgatgcct ggatcgacga cgtggtgaag cgggctgtcg ccgcgggcta cgatgccttc
     2161 gccatcacgg tggataccgc ggcctattcc cgccgcgagc gcgatatcgc cgggcgtttc
     2221 gtcaagccat ggcgggccgc ggcgacgggg cagaactatc agtccgggtt cacctgggac
     2281 aacgtgaagc acttcaagga caagcacggc atcccgttga tcctaaaggg catcgccacc
     2341 ggagaagacg ccgcgctgtg ttgcgagcat ggtgtcgatg gcgtctatgt ttccaaccac
     2401 ggcggccgcc agctcgatca cggccgtggg tcgatggacg tgctgccgga ggtcatggca
     2461 gcggtacggg ggcgcgcgaa ggtgattgtc gacggcagca tctgccgcgg gaccgacatc
     2521 gtgaaggcaa ttgcgctggg tgcggatgcc gtcgcgatgg gtcggatgta ttgctacgcg
     2581 ttggccgcgg atggggaggc cggtgtgcac aagatgctgg aactgctgga gcacgagttg
     2641 ggtgtcgcga tggcgctggc cggcgcccgc ggcctgggcg aactgcatcc cgggtacctg
     2701 ttccgcaatg ctccgcaggt cacgccgacc cacgcccaca gcgctttccc gctattgcaa
     2761 gcgacgcccg agtaccgcgg ctagaccgtt ttccgttcgc ctgggctcac tgaaaacagt
     2821 ctagctcatt gttttagcga gcatcttcac ccgttcagat gatctatttg aacgggatct
     2881 gctctagggt ttgcgcgcgc tgaacgggaa cgtttgcatg gtcaggccga aacagcctcg
     2941 cggcctgacc tgaactgaag aacgcgcggt tcgcgcaggc tacacgacgg gtgtcgccat
     3001 gacatgctgc cgatacgccg ggcgctcgca caagcggtcg taccaggcgc ggagcgcggg
     3061 tatgtccggg cgggtcaggc cgaggtaatc catgttgaac cagcggtgcg catgcacacc
     3121 ccacgggata tcgcacaggg taaagtcgct gccggcaatg aaggcgtgct gcttcaggcc
     3181 agtttcgatc atgcgccatg ctcgggcggt ttctgcgatc gaggcttcca gggccttcag
     3241 atcccgcttg tccggcgcgg tgcggaccag gccccagaac acaccgctca tggggcgatt
     3301 gagcaccgtc tgctgggcgt ccatccaacg gtcgatgttt gccctggcgt gtggctcggt
     3361 gggccaaagg ggggaaccgg acgcatgcgt ctgggcgagg tagcgcagga tggcattgct
     3421 ttcccacagc gtgaacgggc cctcttgcag ggtgggaacc agtccggtcg ggttcatgcc
     3481 gaggtattcc ggggtgtcgg tttttccgaa ggcgccgccg acatcgatcc gctcgaacgc
     3541 caagccgagt tcgcccagcc cccacaggac tttcatcacg ttgctggagg tgttgcgacc
     3601 ccagacaatc cggttcgatg tttcgctcat ggtttcccca gtgtccccga aaaggtgtgt
     3661 ttggaatgcc gccttggacg ctaatccggc cgccgggacc cggcaacttg catccggtcc
     3721 aaggacgggg cacaccgaat ccaatgcagg gtcgtcctcc acgcaacttg gccgttctgg
     3781 ctgccctcgg ggcggcggca gcgcttggag ttgcctttgc tgccgaaacc tggggtgaac
     3841 tggttccttg cgcactgtgt ctgctggagc gctggccata ccgagtcgtc atcgtcctgg
     3901 gcttgctggc cgccgtggtg cccggaggca tcgcgcggat gctgttgatg ctggtaatcg
     3961 tctgcctgct ggccgatgcg gcggtggccg cggtgcatgt cggcgtggag atgggttggt
     4021 ggccgagtcc gctgccggaa tgcgcggcgc cgcgtttcag cggggggtcg atcgccgatc
     4081 gcctcgcgtc aatgccatcg cgacccgcga agccttgcga tgagccgacg tttctggttc
     4141 ccggctttcc cgtgtcgttc gcggcgatga acctgctgtt tgccatggga tttgccgcgc
     4201 tgctggccat gtcgttgtca ggatccattt tgcctcggag gaacaggcga tgacggacga
     4261 acttcccggc gatctttcgg cgcaggagaa aaccaaacgg atgatccggg tggaccatgc
     4321 gggcgagtat ggcgcgaccc gcatctatgc cgggcagctt gccttattgg gtcgtggcgg
     4381 caagggagac ctgctacgcc acatgcaggc gcaggaacag gcgcacctgc atacgttcaa
     4441 cggcatgatt gccgaccgcc gggtgcgccc gacggcattg ctgccgctgt ggcacttggc
     4501 agggttcgct ttgggtgcgg tctcggccgc gatgggcgaa aaagccgcga tggcctgtac
     4561 ggtcgcggtc gaggaagcca tagacgagca ttacagcgcc cagcttgaaa gcctgggtgc
     4621 ggccgaaccg gagcttcggc aaatcgtgga aaaattccgc agcgaagagc tggaacaccg
     4681 cgatctgggc ctggatcacg gtgcggaaca ggccccgggt tatcagctga tgtcccgcgt
     4741 catcaaggcg ggatgccggg cggcgatcgc gatcagcgag cggatataag cgttccgtcc
     4801 gacgcccgtg gggtgcgtag tgggtaattg ccgtgccgcc gtcgcgggca gcctgacgtg
     4861 atcgtcctga tctcgccaca ttttcggcgg cgtttagacg cgaagaggta tataattccg
     4921 cgtcatgacc tgcatcacca acaacttatg cgccggtcga tcatgatcgc cgccctggcc
     4981 tgggccttgc ccgtccgggc tcagccgaag ctgccgcccg gtctgttgga atatgccgcg
     5041 agcgcgctgg cccggctgat cgatgacgcg cgacagcagg ctatcgcgga cggagtgcga
     5101 ccgatccaag caaccaccta ccgcgctttg ctcggctatt ttccgccggc tttattgcag
     5161 cggtgccgtt ttgccaccgg caccagccga acgctgacca tgcccgcgtt agccttttcc
     5221 tacggcgacg cgtcggcgat cacactgggg gacgttgtct tattcaagtc cgaacgtgtc
     5281 gccgacagcg acctgaaggt ttgggcgcac gagttgaccc atgtgatgca gtaccagcgc
     5341 tggggcgtgg acggtttcgc cgaacggtat gtgcgcgata gtgccgcggt ggagcgcgag
     5401 gcgaccgaca acgccaaccg cttcgttacg tggcacgcga cgcaagctgg acgatagatc
     5461 cgcagcacat cgagccgcag cacatcgagc cagagtggcc cgcagcgttc cagcgcttca
     5521 gaccaggacg tttgttcaat tgggccggca cttttgttcg tccaggcggc acttttgttc
     5581 aactgggcct gtacgttcgt tcgatttggg atcgacgcaa cccttccggg cttaaaccgt
     5641 ttaggtcatg cgaccagaag tggccgcaaa acaggagcga aacatggaca aaaatcgagt
     5701 cgaaggcacc gcacaccagg t
//
LOCUS       DASWQN010000868         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1798993, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000868 DASWQN010000000
VERSION     DASWQN010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798993"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..655
                     /locus_tag="VGC82_14650"
     CDS             110..655
                     /locus_tag="VGC82_14650"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEY0424555.1"
                     /translation="MKIDAKISKLRRHIAGLAVIVTAGSLAACTAAAPPGPVVRTGAE
                     TAAISSTVTEPAGYETIYLSGMTGTLRAPDGSPPGTPGPDSGDTEAQATRALGNIKNA
                     LASKGLGFGDIVMMHAYLAPDPQTGRGDSAGWSRAYSKFFGVADQPNKPSRTSITVVL
                     GSPAAKIEIETIAVRKPAGKI"
     gene            778..984
                     /locus_tag="VGC82_14655"
     CDS             778..984
                     /locus_tag="VGC82_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424556.1"
                     /translation="MICHIQYFTKDSKPANQVEWDGEQADVLNAAQDGLVEHKADFAI
                     VFDEEGRNLGTVGDFATNRPWPEA"
     gene            1051..1338
                     /locus_tag="VGC82_14660"
     CDS             1051..1338
                     /locus_tag="VGC82_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424557.1"
                     /translation="MNIKTALLALAAVGFLAAPMMVTTAGADTMKSCAATWKGMAPAD
                     KAMTTHKAFLSSCGEDTPKQMVAVRPDQRLAPLPCHDYSDCTTQEVTSDPK"
BASE COUNT          321 a          462 c          456 g          298 t
ORIGIN      
        1 attcctgcgt taccaaaata aatgcgtcgg gcttccgcac gatttgtgaa gcgagtactg
       61 tcgcgtcgat atcaatctaa ttctgataac ttctgtcgga gcgatggtta tgaaaattga
      121 cgcgaagatt tcgaagctgc gccggcacat tgcagggttg gcagtgattg tcacggccgg
      181 ttcgctggcg gcctgtaccg cagctgcgcc gccgggaccc gtggtccgta ccggggcaga
      241 gaccgcggcg atttcgtcga cggtgaccga gccggccggc tacgagacca tctatctcag
      301 cggcatgacc ggaaccttgc gggctccgga cggttctccg ccaggcacgc cgggtcccga
      361 ttcaggcgat accgaggccc aggcaacgcg tgcgctcggc aacatcaaga atgccttggc
      421 gtccaagggg ctgggctttg gcgatatcgt aatgatgcac gcctatctgg cgcccgatcc
      481 gcagacgggc cgcggcgatt ccgcgggttg gagccgcgct tattcgaaat ttttcggcgt
      541 ggccgaccag cccaacaagc cttcgcgcac ctcgatcacg gtcgttctcg gcagtccggc
      601 ggcgaaaatc gagatcgaga ccattgctgt gcgcaaaccc gcagggaaaa tatgagctag
      661 gacttttggt gaaccgtcat cagttcgccg cgacgagcgc ccaaccacgc ccatgctttt
      721 caataggttc atatggtccg gattgccgtc cttcacgcga ggctttagca ttaagccatg
      781 atctgccaca tccagtattt cacgaaggac agcaagcccg cgaaccaggt ggaatgggat
      841 ggtgagcaag ccgacgtgtt gaacgccgcg caggatggtc ttgtcgagca caaggctgat
      901 tttgccattg tcttcgacga ggaaggccgg aatttgggaa ccgtagggga ctttgctacc
      961 aatcgcccat ggccggaagc ctgaggcttg cggtcgggac tcatgaatca tagccagaaa
     1021 gcgacgatag cgaccaggaa gacggcagcc atgaatatca agaccgcttt actcgccttg
     1081 gccgctgtcg gctttctggc tgcgccaatg atggtcacaa cggccggcgc cgacaccatg
     1141 aaaagctgcg cggcgacctg gaaaggcatg gcgcccgccg acaaggccat gacgacccac
     1201 aaggcgttct tgtcgtcttg cggggaggac accccgaagc agatggttgc agtccgtccc
     1261 gaccagcggc ttgctccgtt gccctgccac gattactccg actgcaccac gcaggaggta
     1321 acgtcggatc ccaagtaagc cgataaatta gtgccagcct ggtgtcccgc gagggcgcag
     1381 caatcttcaa ttccataatg ccaaatttcc aaaggcggac cgccgccccg acggcctggg
     1441 tcccaatggc gtcgccgtta ttctgggctt cctgcgcgcc aaagccccgg tcgctgctgc
     1501 gcccatccat gccggttagc caaccgcgaa ataacct
//
LOCUS       DASWQN010000869         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1271308, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000869 DASWQN010000000
VERSION     DASWQN010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271308"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..429
                     /locus_tag="VGC82_14665"
     CDS             133..429
                     /locus_tag="VGC82_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424558.1"
                     /translation="MGYLVTFQWGWLLLSLLLGFAMGWIAVVQRGRGVSKAAARWLAV
                     LAAALLASALARLIPGRPGYWLDLGLMMFAAYLAGCTAGSWLRARVLSRSTPAA"
     gene            811..1866
                     /locus_tag="VGC82_14670"
     CDS             811..1866
                     /locus_tag="VGC82_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424559.1"
                     /translation="MLEAIRRAISFLRQKQILHKLGVAVSVTVIGIACYVLYHMLRGI
                     DVDQVVEAIKQTEPSQIGLAALFVAAGYFTLTFYDLFAVRAIGRTEVPYRINALAAFT
                     SYSIGHNVGASVFTGGAVRYRIYSAWGLNAIDVAKICFLAGLTFWLGNAAVLGLGISY
                     HPEAAAAIDQLPPWLNRIVALAIIGGLLAYVAWVWMQPRAVGSGPWTVMLPGGPLTLL
                     QIAIGIIDLGFCALAMYVLVPDEPNLGFVVVAVIFVSATLLGFASHSPGGLGVFDAAM
                     LVGLWQMDREDLLAGMLLFRLLYYIAPFVISVILLTFREVILGARMKRLRQASPGLDA
                     GPPREAAYVRKRRDTGG"
     gene            1897..>2695
                     /locus_tag="VGC82_14675"
     CDS             1897..>2695
                     /locus_tag="VGC82_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY0424560.1"
                     /translation="MAIDASSSLFSPWPDRLRHAAIILLAAGLALAFLVAFGELSLFR
                     AAAVFVCIAAAALVPWRLHTTVESREEVRGANPVESPAVSAIVAGMPDPAVLLDRAGR
                     VIHLNAAAAQLAPALRKNELAQFALRSPEIITALREAIATSEPRRATYLDHVPVDRWM
                     ELIITPVPVPTLFGGSDKCMLMTFHDQTPLRRVEEMRADFVANASHELRTPLAALSGF
                     IDTLQGPAKDDAKARERFLGIMHAQATRMARLIDDLLSLSRVELSAHV"
BASE COUNT          401 a          873 c          875 g          546 t
ORIGIN      
        1 ggtcaaggcc ggtttgcccg cgaaccggtt cacggcgatc ggctacggca gtacccagcc
       61 ggtcgccggc aacgatacca acgaaggcaa ggcgcagaac cggcgcatcg atttcctggt
      121 gagataaggg cgatggggta tttggtgacg tttcaatggg gctggctgct gctctcgctg
      181 ctgctgggct tcgccatggg ctggatcgcg gtggtccagc gcggtcgagg cgtgtcgaag
      241 gcggctgccc gatggctggc cgtgctggcg gcggcgttgc tcgcatcggc gcttgcccgc
      301 ctgatccccg gccgtcccgg ctattggctc gaccttggtc tcatgatgtt cgcggcctat
      361 ctcgccggtt gcacggcggg atcatggctg cgtgccaggg tgctctcgcg cagcacgccg
      421 gccgcctgag ctcgccacgt cttcccggcc cgttaccgga tgttacatgc gatgccgacc
      481 ggacttctcg ggcgaggggc cagaactcga tctgctgatc ttccggcggc tctgggccgg
      541 actacggcgc gatatcacga aaacttcact gcttgctccg cgcggttgcg gtggtagctc
      601 ttgccgccct cgggctgaat gcacgatcct gcgcatccgg ggcgccgaac ttcgtcctaa
      661 tgtgttgaac gagcggattt atttacatca agcgaattcc actccggctt aaaacgcgct
      721 accacgtcgt gccggaaagc gagcttggcg ccgacggcgg caaggttggc ctgggcgcgg
      781 tggtcgcccg atcgtatttt tgaggtctag atgctggaag caatacgcag ggcgatctcg
      841 tttctgcgcc agaagcaaat cctgcataag ctaggtgttg cggtcagcgt cacggtcatc
      901 ggaatcgcgt gctacgtgct ttaccacatg ctccggggca tcgatgtcga ccaggtggtc
      961 gaggcgatca agcagaccga gcccagccag atcggcctcg cggcgttgtt cgtggcggcg
     1021 ggctatttca ccctgacatt ctatgacctg ttcgcggttc gcgcgatcgg ccgcaccgag
     1081 gttccgtacc gcatcaacgc gctagcggcc ttcaccagtt attcgatcgg gcacaatgtc
     1141 ggcgccagcg tgttcaccgg gggcgcggtg cgttaccgga tctattccgc ctgggggctg
     1201 aatgcgatcg atgtcgccaa aatatgtttc cttgccggcc tgacgttctg gctcggcaac
     1261 gcggcggtac tgggactcgg gatctcctat catcccgaag ccgccgccgc gatcgatcag
     1321 ctgccgccct ggctcaaccg catcgtcgcg ctcgcgatca tcggcgggct gctggcatat
     1381 gtggcctggg tctggatgca accaagggcc gttggcagcg gcccctggac cgtgatgctg
     1441 cccggcggcc cgctgacctt gctgcagatc gcgatcggga tcatcgatct cggtttctgc
     1501 gcgctcgcga tgtatgtgct ggtgccggac gaacccaatc tcggtttcgt cgtcgtcgcg
     1561 gtgatctttg tctcggcgac gctgttgggc ttcgccagcc attcccccgg cgggctcggc
     1621 gtgttcgacg ccgccatgct ggtcggactg tggcagatgg accgggaaga tctgctggcc
     1681 ggaatgctgc tgtttcgcct gctttattat attgcgcctt tcgtcatatc tgtgatcctg
     1741 ctgacgttcc gggaggttat actgggcgcc cgaatgaaac gtctgcgcca ggcgtcaccg
     1801 gggctggacg ccggaccgcc gcgcgaagcc gcctatgtca ggaaacggcg cgacaccggc
     1861 ggctgagtcc gataggattg cggagatatc cggccgatgg cgatagatgc ttcttcttcc
     1921 ctgttttcgc cgtggccgga ccgcttgcgc catgccgcca tcatcctgct tgccgccgga
     1981 ctggcgctgg ccttcctggt cgccttcggc gaattgtcct tgttccgcgc cgccgcggtg
     2041 tttgtctgca tcgccgctgc cgcgctggtg ccgtggcggc tgcacaccac ggtcgaatcg
     2101 cgcgaggaag tccgcggcgc caatccagtg gaaagcccgg ccgtcagcgc cattgtggcc
     2161 ggcatgcccg accccgcggt gctgctcgac cgcgccggcc gcgtcattca cctcaatgcc
     2221 gccgcggcgc agctggcgcc ggcgctgcgc aagaacgaac tggcccagtt cgcgctgcgc
     2281 tcgcccgaga tcatcaccgc gctgcgcgaa gccattgcga ccagcgaacc ccggcgcgcc
     2341 acctatctgg atcacgttcc ggtcgaccgc tggatggagt tgatcatcac cccggtgccg
     2401 gtaccgacgt tattcggcgg cagcgacaaa tgcatgctga tgacgttcca cgaccagacc
     2461 ccgctgcgcc gggtcgagga aatgcgcgcc gatttcgtcg ccaacgccag ccacgaactg
     2521 cggaccccgc tcgcggcgct gtcgggcttc attgacacgc tgcagggtcc ggccaaggac
     2581 gacgccaagg cgcgcgagcg cttcctcggc atcatgcatg cgcaggcgac gcggatggcg
     2641 cggctgatcg acgacctgtt gtcgctatcg cgggtcgaac tctctgccca cgtcc
//
LOCUS       DASWQN010000870         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1666995, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000870 DASWQN010000000
VERSION     DASWQN010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666995"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VGC82_14680"
     CDS             <1..348
                     /locus_tag="VGC82_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEY0424561.1"
                     /translation="IEVEDAPFIAVELDWTGDGRHQVLSFRTNIDEVVTAGPDHPIRI
                     SQDLITCEPTPYILVRPGSAGHLGIEARINRATYYELVALAVPEWVGSKRLLGVWSCG
                     KFFSLGELPPGEA"
     gene            345..947
                     /locus_tag="VGC82_14685"
     CDS             345..947
                     /locus_tag="VGC82_14685"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY0424562.1"
                     /translation="MTPAALRVRLAEIAAQPFEASAPHGDDVLATLAKAPVPAAVLVP
                     VVLSETPGVLLTKRTSHLSAHAGQVSFPGGRIDPEDSGPEAAALREAWEEIGLEPASV
                     QVLGRMTDHVTGTGYRITPILGVVPPDLVYRLSLHEVESAFELPISVVLDPNAPKLQR
                     QHVGGVWREYWVWPHPDHYIWGATAAILVQLAERLRERAV"
     gene            947..1417
                     /locus_tag="VGC82_14690"
     CDS             947..1417
                     /locus_tag="VGC82_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEY0424563.1"
                     /translation="MRRAFLVTCLALQAGLASFAGSALAQTRIDSINTNFRWLGPDDK
                     IIVERYDDPRVGNVSCYLSRAETGGIKGGLGFAEDPSRFSIACRAVGPVSLPPSMPKN
                     EVIGFSQASLFFKAFQIHRSLDAEKRVLVYTVVSTKLINGSPFNSISVVPTALP"
BASE COUNT          318 a          548 c          614 g          375 t
ORIGIN      
        1 atcgaggtcg aggatgcacc gttcatcgcg gtcgaactcg attggaccgg cgacgggcgg
       61 catcaggtgc tgtcctttcg caccaacatc gacgaagtcg tcaccgccgg tccggaccac
      121 cccatccgca tctcccaaga cctgatcacc tgcgaaccga ccccgtacat cctggtgcgc
      181 cctggttcgg ctgggcacct tgggatcgag gcccgcatca atcgcgcgac ctattacgag
      241 ttggtcgctc tcgcggttcc cgagtgggtg ggtagcaagc gcctccttgg cgtgtggagt
      301 tgcggcaaat tcttttccct gggcgagttg ccgcccggcg aggcgtgacg ccggccgcgt
      361 tgcgggtccg gctggccgag atcgccgcgc aaccgttcga agcgtccgcg ccacatggcg
      421 acgacgtgct ggcgaccctt gccaaggcgc cggtgccggc tgcggtcctg gtgcctgtcg
      481 tcctcagcga gacgccgggc gtcctgctga ccaaacgcac ctcccacctc agcgcgcacg
      541 ctggacaagt cagcttcccg ggtggccgga tcgaccccga agatagcggg cccgaggctg
      601 cggcgctgcg ggaggcctgg gaggaaatcg ggctggagcc tgcgtcggtg caagtgctgg
      661 ggcggatgac cgaccatgtg acgggcaccg gctatcggat tacgccgatc ttgggcgtag
      721 tgccgcccga cctggtttac cggttgtcgt tgcacgaggt ggaatcggcg ttcgaattgc
      781 cgatcagcgt cgtgttggat ccgaacgccc cgaagttgca gcgccagcac gtcggggggg
      841 tgtggcgcga atattgggtt tggccgcacc ctgatcatta tatctggggc gccaccgcgg
      901 cgatcctggt tcaactggcc gaacggttgc gtgaaagggc tgtctgatgc gtcgcgcgtt
      961 tttagtcacc tgcttggcat tgcaagccgg gctggcgtcg tttgccggtt cggcattggc
     1021 gcagaccagg atcgattcca tcaacacgaa cttccgttgg ctagggccgg acgacaagat
     1081 catcgtggag cggtatgacg atccccgcgt cggcaacgtg tcctgctatc tgtcgcgggc
     1141 cgagacgggc gggatcaagg gcggcctggg gttcgccgag gacccaagcc ggttctcgat
     1201 cgcctgccga gcggtgggac cggtatcgct gccgccgtcg atgccgaaga acgaggtcat
     1261 cgggttctcg caggcatcgc tgttcttcaa ggccttccag atccaccggt cgctggatgc
     1321 cgagaagcgt gtcctggtct atacggtggt cagcaccaag ctgataaacg gctcgccgtt
     1381 caacagcatc agcgtggtgc cgacggcttt gccttgacgc ctggttcccg gggatcaggt
     1441 ggatcgtccc accagtcggc gctgatggga tgaaactgac tgaacctggt caggatcatc
     1501 catccatgcg cgcgatcgtt gaaaagcaag gcatgcgcgg gtaccagagc ggcggacaat
     1561 gaagcggtgg ataatgatca tcccggttga cggacgagat ttcatcctgg gaccagcggc
     1621 ctccggcgtg ccgccagctt cgcgattggc ggaggcgtcc tgctcatgtc ggcacgatgt
     1681 ggaagagatt acaagcactg gcccggttga gtatcgtcgt agtgcgaacc cgtggacaac
     1741 tcgtggggat tttgttgcga accaaaggga ttgaattttt agctgccact agtccgttat
     1801 aagagcatgc tacttcctct taatcctgtt ttgtggaaca atgcttgggc cgtta
//
LOCUS       DASWQN010000871         3990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2195100, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000871 DASWQN010000000
VERSION     DASWQN010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195100"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VGC82_14695"
     CDS             complement(<1..323)
                     /locus_tag="VGC82_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424564.1"
                     /translation="MATTSNEGEIVMAMRILWPNLPAQLVPVATEAVGPGFETDFHEK
                     FEDVTDEQWANANAVVGACPPQYIDKLRNCRIFVKYGVGYDDVDIERFGKLGIPVCNV
                     PDYGTR"
     gene            complement(801..1928)
                     /locus_tag="VGC82_14700"
     CDS             complement(801..1928)
                     /locus_tag="VGC82_14700"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0424565.1"
                     /translation="MNVDVLNRSGIQTAAASRLAIADCDIHPRPAGVGIGGVSKSLYP
                     YLSKRWVDHVEEFGITYRQPWEKGSAYPKGQPQACRRDAFLPDGRSPGSDLRFMAEQH
                     LDPNNVALGILNPLTSGQGATNPLLSDAITHASNEWQKAEWTSQDKRLRGSVVVPYED
                     AAASVREIELRAGDADFAQVLLLSRTAEPLGQRRYWPIYEAAAAANLAVGIHAFGNGG
                     WPNTAGGWGSYYIEEMVGHAQAQQALLVSMILEGVFEHVPDLRVVLIEGGLAWAAALA
                     WRLDRQWAKLKRELPHLKRAPSEYIRTNVWFTTQPIEEPEPRTQLAEAIEWIGWDRVL
                     FATDYPHWDFDDPAQALPIKLTEAQRRAVFMDNAKAVYRQG"
     gene            complement(2034..3110)
                     /locus_tag="VGC82_14705"
     CDS             complement(2034..3110)
                     /locus_tag="VGC82_14705"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0424566.1"
                     /translation="MTGVAPIDCDVHPVVPELAALMPYMDDHWRETVVRRGIEGLTTI
                     SYPTRNPLSFRQDWRDETGRTATDAATLGRQGLDLFGTRLAICNCLYGVQAQFSEDLG
                     MAMSRALNDWIVREWLDRDPRLRASIVVPMQNPELAVDEINRCAPDRRFVQVMLLVGS
                     EITLGRRQNWPIYAAAERHGLPIGIHAGSMYRNPMTPVGWPTSFTEEYVNQATAFQSQ
                     LTSLIAEGVFAKFPSLTVVLMESGVSWLPAYLWRLTKFWKGLRSEIPWVSDPPGSIVR
                     DRVRLTLQPFDAPPDASSVMRLIEHIGSDEMLLFSTDYPHYQFDGDSLTGAGPIPEGM
                     DKNLAQKIMAENPLRTYVRLNEIA"
     gene            complement(3247..3564)
                     /locus_tag="VGC82_14710"
     CDS             complement(3247..3564)
                     /locus_tag="VGC82_14710"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY0424567.1"
                     /translation="MSDWVRVAAKGDVAEGACIGVRVGDREVAVYHLPGGEFRATANV
                     CTHEYALLSDGWLENGCIECPLHAAQFDIRTGKALSAPADEDIAVYPLKAEGEDLLVQ
                     VPG"
     gene            complement(3656..>3990)
                     /locus_tag="VGC82_14715"
     CDS             complement(3656..>3990)
                     /locus_tag="VGC82_14715"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0424568.1"
                     /translation="IVNISSVAAIVGTGSNIAYGGSKAALDTMALSLARILGPEIRVL
                     TVSPAAVDTAFVPGRTTAMVEKVASTTPLKRVVQADEVAQAVMAAVVNLTSTTGWIIP
                     VDGGKLVG"
BASE COUNT          714 a         1283 c         1269 g          724 t
ORIGIN      
        1 cgcgtgccgt aatccggcac gttgcagacg gggatgccga gtttgccgaa gcgctcgata
       61 tcgacgtcgt cgtagccgac gccgtatttc acgaagatgc ggcaattgcg cagtttgtcg
      121 atgtattgcg gggggcaggc gccgacgacc gcatttgcgt tggcccattg ctcgtcggtg
      181 acatcctcga atttctcgtg gaagtccgtc tcgaaccctg ggccgaccgc ttcggtcgcg
      241 actggtacaa gctgtgccgg caggttcggc cagagaatgc gcatcgccat gacaatttcc
      301 ccctcgttcg atgtcgtggc catggtgcca tcgccacagc ggaatgacca catgcgagcg
      361 cgaaaccgag cggcctgtcg tccatcgatg cctcctggca ggcccatgtc ggtcaaggcg
      421 ggggcggctg atcgtctgcg ttcaggaaat ggatcggatt gttggccgga ccgccatggg
      481 cgcaaccgga cgtgtccgac acgagccttt ggagcggttc acggcgactg gaaacagggg
      541 ttctctcgcg gctgcaaagg aagtcgtggg tggccgcgcc aagcacggcc atgacacata
      601 ggcgtggcga cacataggcg tggcggcacg ggtcggcttg caccctggaa tgggcgcata
      661 gggcgatgcc cctcgccgcc atcgcccgag cgccggtttt gcgatgacag gaacgcatcg
      721 caaccgaagc tgtcgctgcc gcgataagac gcggcccatg acgcaccggc tgccgccgac
      781 gcgtctttca cccagattga tcagccctgc cgataaaccg ccttggcgtt gtccatgaac
      841 accgcgcggc gttgcgcctc ggtcagcttg atgggcagcg cctgcgcggg atcgtcaaaa
      901 tcccaatgcg gatagtcggt cgcaaacaac acccgatccc agccgatcca ctcgatcgcc
      961 tcggccaact gggtgcgggg ttcgggttcc tcgatcggct gggtggtgaa ccagacgttc
     1021 gtgcggatgt attccgaggg cgcccgcttc aaatgcggca gttcccgctt cagcttcgcc
     1081 cattgccggt ccagccgcca agccaacgcg gcggcccagg ccaacccgcc ctcgatcagc
     1141 acgacgcgca agtcgggcac gtgttcgaat acgccctcca gaatcatgct gaccagcagc
     1201 gcctgctgcg cctgagcgtg accgaccatt tcctcgatgt agtagctgcc ccagccgccg
     1261 gcggtattcg gccagccgcc attgccgaac gcatggatgc cgacggccag gttcgcggcg
     1321 gcggcggctt cgtagatcgg ccaatagcgg cgctgcccga gcggttcggc ggtgcgtgac
     1381 agcagcagga cctgggcgaa gtcggcatcg ccggcgcgca gttcgatttc gcgcaccgag
     1441 gcggcggcgt cctcgtatgg gacgacgacc gaaccgcgca ggcgcttgtc ctgcgatgtc
     1501 cattcggcct tctgccattc gttcgacgcg tgggtgatgg cgtccgacag cagcgggttg
     1561 gtcgcgccct ggccgctggt cagcgggttc aggatgccaa gtgccacgtt gttcgggtca
     1621 agatgctgtt cggccatgaa gcgcaaatcg ctgccgggcg agcggccatc gggcaggaac
     1681 gcatcgcggc ggcaagcctg cggctggccc ttgggatagg cagagccctt ttcccatggc
     1741 tgacgatagg tgatgccgaa ctcctcgacg tggtccaccc agcgcttgga cagataggga
     1801 tacagcgact tgctgacccc accgataccg actccggccg ggcggggatg gatgtcgcag
     1861 tcggcgatgg ccagccgcga tgcggcggcg gtctggatgc cggagcgatt gaggacgtcc
     1921 acattcatgg cgtgtctccg ttggttttga tcgcctgatc cacgcgttcc gcacgtttcg
     1981 cgcaacgcga tcagtagctt ggtcgcctga tccacgcgtt ccgcgcaacg cgatcaggcg
     2041 atttcattga gtcgaacata agtccgcagc gggttttccg ccatgatctt ctgcgcgaga
     2101 tttttgtcca tcccttccgg aatcggtccc gcgccggtca aactgtcgcc gtcgaactgg
     2161 tagtgcggat agtcggtgga gaacagcagc atctcgtccg acccgatatg ctcgatcaac
     2221 cgcatgaccg aggacgcatc aggcggcgca tcgaacggct gcaaggtcag acgcacgcgg
     2281 tcgcggacga tggaccctgg cggatcggag acccatggga tctcgctgcg cagccccttc
     2341 cagaacttcg tcaggcgcca caggtaggcg ggcagccagg agacgccgga ttccatcagc
     2401 accacggtca acgaggggaa tttcgcgaag acaccctcgg cgatcaggct ggtaagctgg
     2461 gattggaagg cggtggcctg attgacgtat tcctcggtga agctggtcgg ccagccgaca
     2521 ggggtcatcg ggttgcgata catcgaaccg gcatggatgc cgatgggcag accgtgccgt
     2581 tcggccgccg cgtagatcgg ccagttctgc cggcggccga gggtaatctc actgccgacc
     2641 agcagcatga cctggacaaa acggcggtcc ggcgcgcagc gattgatctc atccaccgcc
     2701 agttcggggt tctgcatcgg cacgacgatg gacgcacgca accgcggatc gcgatccagc
     2761 cattcccgaa caatccagtc attcagcgct cgggacatcg ccatgccgag gtcttcgcta
     2821 aattgcgcct gcacgccata aaggcagttg cagatggcta aacgggttcc gaacaggtcc
     2881 agcccctgtc gcccgagggt cgctgcgtcg gtggcggtgc ggccggtttc gtcgcgccaa
     2941 tcctgccgga aactgagcgg attgcgcgtc ggatagctga tcgtcgtgag gccctcgatg
     3001 ccgcggcgca ccacggtttc gcgccagtga tcatccatgt agggcatcag cgcggcgagt
     3061 tccggcacga ccgggtgaac gtcgcaatcg atcggggcga ccccggtcat cgctgtcgac
     3121 gccctgtcgg ccccggaccg caaccacccg atcggctgtc gctccatgct tcaacgtcat
     3181 cgcaggcagc ggccagcggt ccgggcgtcc gcttgcaatg aagcggactg aagccaagat
     3241 cggcgatcat cccggaacct gcaccagcaa atcttcccct tcggctttca ggggataaac
     3301 ggcgatgtct tcatcggctg gcgcggagag ggctttgccg gtgcgaatgt cgaattgcgc
     3361 ggcatgcagc gggcactcga tgcaaccatt ctccagccaa ccgtcggaca gcagcgcata
     3421 ttcgtgcgtg cagacgtttg ccgttgcgcg aaattcgccc cctgggaggt ggtaaaccgc
     3481 gacttccctg tcacccactc gcacgccgat acacgcacct tccgcaacgt cacccttcgc
     3541 ggctacccgc acccagtcgc tcatcgtcgt ctccatcaat gtcgccggac agcataccag
     3601 tggatacagc atgccaatgg atacagcatg ccagtggatt atgggttggc caacctcagc
     3661 ccaccagttt gccgccatcc accgggataa tccagccggt ggtggatgtc aggttgacca
     3721 ccgccgccat gaccgcctgc gccacctcat ctgcctggac cacgcgcttc agcggcgtgg
     3781 tcgaggcgac tttctccacc atcgccgtcg tgcgccccgg cacgaaagcc gtgtccaccg
     3841 cggcggggga gacggtcagc acccggattt ccggtcccag aatgcgcgcc agcgacagtg
     3901 ccatggtgtc cagtgcggct ttcgacccgc cataggcgat gttgctgccg gtgccgacga
     3961 tcgccgcgac cgaagagatg ttcacgatca
//
LOCUS       DASWQN010000872         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2195164, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000872 DASWQN010000000
VERSION     DASWQN010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195164"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VGC82_14720"
     CDS             <1..334
                     /locus_tag="VGC82_14720"
                     /inference="COORDINATES: protein motif:HMM:NF037716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HEY0424569.1"
                     /translation="SSDWGNVRAELADNAAARLLIQMLPITIEMQDHLRQEKTGNLPS
                     SLPEVPRQRDFAVGTLGLWGPDHFVIYYRKGRVPQPGIAILGNVTGDVSIFDRPGPIT
                     VRVEAVNP"
     gene            346..648
                     /locus_tag="VGC82_14725"
     CDS             346..648
                     /locus_tag="VGC82_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424570.1"
                     /translation="MKGRLSASNALLALIALLNLLSAATLAQPANLPPALVYDTLTHG
                     PVPIPLGEKHWHGATTTTAMSPFRRSSTAKTSIGWKRSLTNNSRADRAADCRRPAT"
     gene            658..984
                     /locus_tag="VGC82_14730"
     CDS             658..984
                     /locus_tag="VGC82_14730"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0424571.1"
                     /translation="MAQEPTNARKSFGDIAPHLAEITDKVLFGDVWANPALSPRDRSL
                     VTITSLISLYRTNELPFHLNGAVENGVTKDEIIATITHLAFYAGWPPAMTGMQIARKV
                     FDEADS"
     gene            996..2198
                     /locus_tag="VGC82_14735"
     CDS             996..2198
                     /locus_tag="VGC82_14735"
                     /inference="COORDINATES: protein motif:HMM:NF009048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HEY0424572.1"
                     /translation="MSATCSASRPAPSVMTALTPIMVTVLVGFVVIGAALPVLPLHVR
                     DTLGFGPVMVGVVAGCQFAAALVARLWSGHAADTKGAKWAVMAGLLAATAAGLLYLMS
                     LSFVSTPTISVVILLAGRAVLGGAESFIITGATTWGLVRVGAHNSGKVIAWMGTAMFA
                     AFAGGAPLGTVLYTNGGFGAVALATAIAPLATLLLIAPLRGATPAKKGGPSILSVVSR
                     IWLPGVGAALGSVGFGAIIAFSSLLFVEHGWTPVWLPFTAYAVALILARLLFGHLPDR
                     IGGAQVALISVLVETTGLTLMWLAPSALVAATGAALTGFGYSLVFPGLGVEAIRLAPP
                     ESRGVAMGAYTACLDIALGVSGPVLGLIASSAGFGVVFLVSAIVVLSSAVVALKLQRK
                     ALAVIEAR"
     gene            2198..2452
                     /locus_tag="VGC82_14740"
     CDS             2198..2452
                     /locus_tag="VGC82_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0424573.1"
                     /translation="MNRRTLAACICAFLAADLNRSVIAEEVHMPLVRIAQLEIDPERL
                     ESYTAAVKEEFETSIRVEPGVLAIYAVAEKDNPSKLRVCL"
     gene            complement(2456..>2846)
                     /locus_tag="VGC82_14745"
     CDS             complement(2456..>2846)
                     /locus_tag="VGC82_14745"
                     /inference="COORDINATES: protein motif:HMM:NF024975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0424574.1"
                     /translation="KYGFPLAITVSPANVHDSKGIVPVLHQLAGRGFKGTALGDLSYR
                     GQRLSKASEALGITLQPSAGGHGGTFIPEGIRWVVERSLAWISRYRRLNTIFERTEGH
                     LTAFIEIAFISILSRRLVRLVTQEISA"
BASE COUNT          489 a          874 c          890 g          593 t
ORIGIN      
        1 ttcatcagac tggggaaatg tcagggccga gctggccgac aacgctgcag cgcgtttgct
       61 gatccagatg ctgccaatca ccatcgagat gcaggatcac cttcgtcagg agaagacggg
      121 caacctgccg tcatcgctgc cggaggttcc gcgtcagcgg gacttcgcag tggggaccct
      181 ggggctatgg gggccggatc atttcgtgat ctactatcgc aaaggccgtg tcccccagcc
      241 cgggattgcg atcctcggca acgtgaccgg cgatgtctcg atctttgacc gtcctggtcc
      301 gattactgtt cgcgtcgagg ccgtgaaccc gtgaggcgat ctggcgtgaa gggccgcctg
      361 agcgcgagca acgcgttgct cgcgctcatt gccctgctga accttctgtc ggcggccacc
      421 ctggcccaac cggctaacct gccgccggcc ctggtctacg acactctaac gcacggaccg
      481 gtgccgatcc cgctcggcga aaagcattgg cacggcgcca cgacgaccac ggccatgtcg
      541 ccgttcagga gaagctcgac ggcaaaaacg tcgattggat ggaaaaggtc actgacgaac
      601 aattcccgcg ctgaccgagc cgccgattgt cggcggcccg caacttagga aaagcacatg
      661 gcccaagaac ccaccaatgc ccgcaaatcg ttcggcgaca tcgcgcctca tctggccgaa
      721 attaccgaca aggttttgtt cggagacgtt tgggcaaatc cggcgctgtc gccgcgcgac
      781 cgtagcctgg tgacgatcac gagcctcatc tcgctctacc gaaccaacga gctgccgttt
      841 cacctgaatg gagcggtgga gaatggcgtt accaaagatg agatcatcgc gacgatcacg
      901 cacctcgctt tctatgcagg ttggccgcct gcgatgaccg gcatgcagat cgcccgcaag
      961 gttttcgacg aagccgacag ctgatccagc cccaaatgtc agcaacatgt tcggcctctc
     1021 gccctgcgcc ttccgtgatg actgctctca cgcctatcat ggtgacagtc ctggttggct
     1081 tcgtggtgat tggtgcagca ctgccggtcc tgccactcca tgtccgcgac accttgggct
     1141 tcgggcctgt catggtcggg gtcgtggcag ggtgtcagtt cgctgcggca ctcgtcgcgc
     1201 gtctctggtc tggccatgcg gcggatacga agggcgccaa gtgggccgtg atggcgggcc
     1261 tcctggcagc cacggccgcc ggattgctct atctcatgtc attaagcttc gtgagcacgc
     1321 cgacgatctc ggtcgtgatc ctgttggccg ggcgcgccgt cctcggcggg gccgagagct
     1381 tcatcattac aggggcgacg acatgggggc tggtccgcgt cggcgcgcat aactccggta
     1441 aggtgatcgc ctggatggga acggcgatgt ttgcggcctt tgccggtggc gcccctttgg
     1501 gcacggtctt atataccaac ggaggcttcg gcgcggtcgc attggctacg gccatcgcac
     1561 cgcttgccac cttgcttctg attgccccgc ttcggggtgc tacgcctgca aaaaaaggcg
     1621 gcccgagcat cctgtccgtc gtcagtcgga tttggttacc tggagttggt gccgccctgg
     1681 gtagcgtcgg tttcggcgcc atcatcgctt tcagctcact gctcttcgtc gagcacgggt
     1741 ggacccctgt ctggttgccc ttcacggctt atgccgttgc cctcatcctc gcacgcctgc
     1801 tgttcggcca tttacccgat cgaattggcg gcgcgcaggt cgcgttgatc tccgtgctgg
     1861 ttgaaacgac tggcctgacg ctgatgtggc ttgcgccatc ggccctcgtg gcggcaaccg
     1921 gcgcagctct gaccggcttt ggatactctc ttgtgtttcc tggcctcggc gtggaggcca
     1981 ttcgccttgc tccgcccgag agccgcggcg tcgccatggg cgcctacacg gcctgcctcg
     2041 acatcgcgct aggtgtctcg ggccccgttc taggcctgat tgcgagtagc gcagggtttg
     2101 gcgttgtctt cctcgtcagt gcaatcgtcg tgctcagttc ggccgtcgtc gccttgaaat
     2161 tgcaacgcaa ggctttggcc gtgatcgagg caagataatg aaccggcgaa cattggcggc
     2221 ttgcatctgc gcgttccttg ctgcggacct gaaccgcagc gtcattgccg aggaggttca
     2281 catgccactt gtccgtattg cccagcttga gatcgatcct gagcgattgg aaagttacac
     2341 ggcggcagtc aaggaagagt tcgagacctc gatccgtgtc gagcccggcg tgctggcaat
     2401 ctacgctgtc gctgaaaagg ataatccttc taaacttaga gtctgtttgt agacgtcagg
     2461 cgctgatttc ctgagtcacc aggcggacca ggcggcggga caggatggag atgaaggcga
     2521 tctcgatgaa agcggtgagg tgcccctcgg tccgctcgaa gatagtgttc aaccggcggt
     2581 agcggctgat ccaggccagg gagcgctcaa cgacccagcg aatcccttcc ggaatgaagg
     2641 ttccgccatg gccgccggcg ctgggttgga gggtgatgcc aagcgcctcg ctggccttgg
     2701 acagccgctg gccgcgatag ctgagatcgc ccagggcagt ccccttgaag ccgcggccgg
     2761 caagttgatg cagcaccgga acgatgccct tgctgtcatg gacattcgcc ggtgagacgg
     2821 tgatcgccag cgggaaaccg tacttg
//
LOCUS       DASWQN010000873         2285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_875885, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000873 DASWQN010000000
VERSION     DASWQN010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875885"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1937
                     /locus_tag="VGC82_14750"
     CDS             <1..1937
                     /locus_tag="VGC82_14750"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0424575.1"
                     /translation="LVRDGWEMLRRLKRAKIPVLWNTSLDRLEGDDRVRAAIAGKHRF
                     EVDVVALNMGFQPEVGLARALGVPHRFVDRGVGYLAAETDSEGRTAVPGVFVVGDGAA
                     PGGARIAEARGRLAGSAAAREVGFAAPPDAATRAALARAETFQDALWRIFAAPAIPIA
                     DSTIVCRCEEITAARLRAEIAVGITSLPALKKATRAGMGRCQGRFCAATIARMCPADP
                     EPEGFAAPRAPLRPVPAAPLMRESPEFSAPLLLDPAPPVRLHALPPLPVETRHAAVAV
                     IGAGVAGLSTAYFLARGGADVLLIDRDEAGMAASTANAGSLHVQLLSYDFNGDTPEDG
                     GPAAHTLALAPRSIALWKAIAEAAGEDLGIRIEGGLMLAEDAAGLAWLRRKSAMERRW
                     GVDSHVLGRNELRDLAPALSDHMLGANFVPEEGYGDPLRGMNALLRQAQAQGVRLLRG
                     AEVCSIDRSGSRWAIATTKGVVTAGVVVNATGPWAARSGRMVGLNLPVTGTVQQVIVT
                     EPAPLLTRHLVAMANRHLSLKQQASGGFLIGGGWFGAFDPATGQSSTLRRSIEGNLWV
                     CAQALPALKALSVLRTWTGINPAIDRAPLLGEAPGLPGFYNAVTANGYTLGPVVGQIT
                     AEAILHGETIDPRYTLARFG"
BASE COUNT          371 a          755 c          783 g          376 t
ORIGIN      
        1 atctggtgcg cgacggctgg gagatgctgc gccgattgaa gcgggcaaag attcccgtgc
       61 tgtggaatac atccctcgac cggctggagg gcgacgaccg ggtgcgggcg gcgatcgccg
      121 gaaagcatcg cttcgaggtc gatgtcgtcg cgctgaacat gggcttccag ccggaagtcg
      181 gcttggcccg cgccctgggt gtaccgcatc ggttcgtcga tcgtggggta ggctatctgg
      241 cggccgagac cgactcggag ggccgcaccg ccgtcccggg cgtgttcgtt gtcggcgatg
      301 gcgcggcacc gggcggtgcc aggatcgccg aagcgagggg acgcctcgcc ggatcggcag
      361 ccgcgcgaga ggtcgggttc gccgcgccgc cggacgcggc cacacgagct gccttggcac
      421 gtgccgaaac cttccaggac gccttgtggc ggatcttcgc cgcccccgcg atcccgatcg
      481 ccgacagcac catcgtttgc cgctgcgaag aaatcactgc cgccaggctg cgcgcggaaa
      541 tcgccgtggg catcacctcc ttgccggcac tgaagaaggc aacccgcgcg gggatgggcc
      601 gctgtcaagg ccggttctgc gccgccacaa tcgcccggat gtgcccggcc gatcccgaac
      661 cggaaggctt cgctgccccg cgtgcgccgt tgcgtccggt gccggccgcg ccattgatgc
      721 gggaatcgcc ggagttctcc gcgcccttgc tgctcgatcc ggcgccgccg gtcaggttgc
      781 acgcgctgcc accactgccc gtcgagaccc gccatgccgc tgtggccgtc atcggcgccg
      841 gcgtggcggg actatcgacc gcctacttcc tggccagggg cggcgcggac gtgctgctga
      901 tcgaccgcga cgaggctggg atggcggcca gcaccgccaa cgccggcagt ctgcacgtcc
      961 agttgctgtc ctacgacttc aatggcgaca cgccggaaga tggcggcccg gcggcgcaca
     1021 cgctggcctt ggccccacgc agcatcgcgc tgtggaaagc catcgctgaa gccgccgggg
     1081 aagacctcgg catccgcatc gaaggcggcc tgatgttggc cgaggacgct gccggtctgg
     1141 cttggctgcg ccgaaagtcg gcgatggaac ggcgctgggg cgttgatagc cacgttcttg
     1201 gaagaaacga actgcgcgac ctcgcgccag ctctgtcgga ccacatgctc ggagccaatt
     1261 tcgtgccgga agagggctac ggcgacccgt tgcgcggcat gaacgctctg ctgcggcagg
     1321 cgcaggcaca gggcgttcgc ctcctgcgtg gggcagaggt ctgcagtatc gatcgttccg
     1381 gatccaggtg ggcgatagca accaccaaag gcgtcgtcac cgcgggcgtt gtggtcaacg
     1441 ccaccggccc ctgggcggcc cgatccggcc gaatggtggg tctgaacctg ccggtgaccg
     1501 gcacggttca gcaggtcatc gtgaccgaac cagcgccgct gctcacgagg catctggtgg
     1561 cgatggcgaa ccggcatctg tcgctgaaac aacaagccag cggtgggttc ttgattggcg
     1621 gcggctggtt cggcgcgttc gatccggcaa ccggtcaaag cagtaccctc cgccggagca
     1681 tcgagggcaa tctgtgggtc tgcgcgcagg ccttgccggc gttgaaggcg ctgtccgtcc
     1741 tgcgcacctg gaccgggatc aatccggcga tcgaccgggc gccattgctg ggggaggcgc
     1801 ccggactgcc cggcttctac aacgcggtca ccgcgaacgg atacacgctg gggccggtgg
     1861 tcgggcagat caccgccgag gcaatcctac acggtgagac gatcgatcca cgctacactt
     1921 tggcgcggtt cggttaagcg tccgttgatc aacgaccgct gcgaaccggg tgatttggct
     1981 caacaacgtc gcggtcggct tcacaccttg cgcgccccat tgatcgccat ggcgcggtcc
     2041 ttgccgcttg tgtcatgacc agccttgtgc cggtacccac gacattcgct gttgaggcaa
     2101 agtcgcgggt gccgcgccaa gcatggccat gacacgatct caaacactac agcctttgtt
     2161 caccatgacg gacttgagac cgcgcttgga gcggggttac ttacggacaa accctaaccg
     2221 ctcagagcca gtggaacccg cgcgccatgg cggtcagcaa gccaccgatc gtggcaacct
     2281 gaagc
//
LOCUS       DASWQN010000874         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_742290, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000874 DASWQN010000000
VERSION     DASWQN010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742290"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /gene="bchL"
                     /locus_tag="VGC82_14755"
     CDS             complement(<1..826)
                     /gene="bchL"
                     /locus_tag="VGC82_14755"
                     /EC_number="1.3.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin:protochlorophyllide reductase
                     (ATP-dependent) iron-sulfur ATP-binding protein"
                     /protein_id="HEY0424576.1"
                     /translation="MTVIRRIPARGDGEGSVQVQMQPDLKIGNAQVFAVYGKGGIGKS
                     TTSSNLSVAFSKLGKRVLQIGCDPKHDSTFTLTKRLVPTVIDILEQVNFHSEELRVED
                     FVYQGYNGVMCVEAGGPPAGTGCGGYVVGQTVKLLKEHHLLEDTDIVIFDVLGDVVCG
                     GFASPLQHAGRALIVAANDFDSIFAMNRIVAAIQAKARNYEVRLAGVIVNRSAATDQI
                     DKYNERTGLKTLAHFPDLDVIRRSRLKKSTLFEMDPTPEIVAVQNEYLRLAAALPAG"
     gene            complement(823..>2300)
                     /locus_tag="VGC82_14760"
     CDS             complement(823..>2300)
                     /locus_tag="VGC82_14760"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEY0424577.1"
                     /translation="LHADPEQLAAQLLDLEYTLIPHGLHVVGEGLNVEQRVAMLDAAG
                     VTDAGERARLDGLLAEDHEVPAIIHALDGGYIRPAPGGDLLRTTEVLPTGRNLHGFDP
                     FRIPSAFAVRDGARQADRLLARHQADSGSYPATVAMVLWGTDNLKTEGGPIAQAIWLL
                     GAEPRCDSYGRVSGAKLVPLETLGRPRIDVIATLSGIFRDLLPLQTKLLAEAALLAAT
                     ADEPIEQNFVRLHALAFQAENGGSIEDAALRVFGNADGAYGANVNQLVGSGTWNDEGE
                     LADAYLKRKGFAYGVSGKPRRHDAVLSHALARVDIAYQNLDSLELGVTTVDHYFDTLG
                     GISRAVRRARGGQAAAVYIGDQTRGDGTVRTISEQIALETRTRALNPKWYEALLSHGY
                     EGVREIEAHVTNTLGWSATTGEVQPWVYEKLSETFVLDPAMRERLAALNPTASAKIAN
                     RLIEAHERNYWLPGDAMREALQHAGEELEDRLEGVGLEVAA"
BASE COUNT          402 a          743 c          705 g          450 t
ORIGIN      
        1 tccccgctgg aagtgctgcc gccagccgga gatattcgtt ctgcaccgcc acgatttccg
       61 gtgtcgggtc catctcgaac agcgtggact tcttcaaccg ggaccggcgg atcacgtcga
      121 ggtcggggaa atgcgccagc gtcttcaaac cggtgcgttc gttgtacttg tcgatctggt
      181 cggtggcggc actgcggttg acgatgacgc cggccagccg cacctcgtag ttgcgggcct
      241 tcgcctggat cgccgcgacg atccggttca tcgcgaagat cgagtcgaaa tcgttcgcgg
      301 cgacgatcaa ggcgcgtccg gcatgttgca gcggggaagc gaatccgccg cacacgacat
      361 ccccaagcac atcgaatatc acgatgtcgg tgtcttcgag cagatgatgt tccttcagca
      421 gctttaccgt ctgcccgacg acatagccgc cgcaaccggt gcccgctggt ggaccgccgg
      481 cctcgacgca catcaccccg ttgtagccct gatagacgaa atcctcgacg cggagttcct
      541 cggaatggaa gttcacctgc tccaggatgt cgatgacggt cggcaccagc cgcttggtca
      601 gggtgaaggt cgaatcgtgc ttcggatcgc aaccgatttg cagcacccgc ttaccaagtt
      661 tggagaacgc gaccgaaaga tttgacgacg tcgtgctttt gccgatgccg cctttgccat
      721 agaccgcgaa tacctgggcg ttgccgatct tgaggtcggg ctgcatctgc acctgaacac
      781 tgccctcccc gtctcctctt gctggaattc gcctgatgac ggtcatgctg ctacctccag
      841 accgacccct tcgaggcggt cttcaagttc ctcaccggcg tgctgcaggg cttcccgcat
      901 cgcatcgcct ggcaaccagt agtttcgctc gtgcgcctcg atcagccgat tggcgatctt
      961 cgccgaggcg gtagggttca gtgccgccaa ccgttcccgc atcgccggat cgaggacgaa
     1021 ggtttccgac agcttctcat agacccaggg ttggacttca ccggtggtgg ccgaccagcc
     1081 aagggtattg gtcacatgcg cttcgatttc ccggacgcct tcatagccgt gcgacagcag
     1141 cgcctcgtac catttcggat tcagtgcgcg agtccgcgtt tccaacgcta tctgttccga
     1201 aatggtacgc accgtgccgt cgccgcgcgt ctgatcgccg atatacacgg ccgccgcctg
     1261 tccaccgcgt gcccgccgta ctgctcggct gatgccaccg agcgtatcga agtaatgatc
     1321 gacggtggtg acgcccagtt cgagcgaatc aaggttctgg tacgcgatat ccacgcgagc
     1381 cagcgcgtgc gacagtaccg cgtcgtgacg tcggggtttt ccggaaacgc cgtacgcgaa
     1441 acctttgcgc ttcaggtagg catcggcgag ttcgccctca tcgttccagg tgccgctgcc
     1501 cacaagttgg ttgacgttgg cgccataggc accgtcggca ttgccgaaca cccgcagcgc
     1561 ggcatcctcg atcgagccgc cgttctcggc ctggaaggcc aaggcgtgca ggcggacaaa
     1621 attctgttcg atcggttcgt cggcggtggc ggccagcagc gcggcctcgg cgagaagttt
     1681 cgtttgcagc ggcagaagat cgcggaaaat cccggacaag gtggcgatca cgtcgatgcg
     1741 tggccgcccc agcgtttcca acggcaccag tttggcgccc gaaacccggc cataactgtc
     1801 gcatcgcggt tccgcgccca acagccatat cgcctgggca atcggacccc cctcggtctt
     1861 gaggttgtcg gtcccccata acaccatcgc cacggttgcc ggatacgagc cgctgtcggc
     1921 ctgatgacgg gcgagcagcc ggtcggcctg gcgggcaccg tcgcgcaccg cgaaggcgct
     1981 ggggatgcgg aaagggtcga agccgtgcag gtttcgtccg gtaggcagca cctccgtggt
     2041 gcgcagcagg tcgccgccgg gtgctgggcg gatgtagccg ccgtccagcg cgtggatgat
     2101 agcgggaacc tcgtgatcct ctgccagcaa tccgtccagc cgcgcgcgtt cgcctgcatc
     2161 cgtcacgccg gcggcgtcca gcatcgcgac acgctgctcc acattcaggc cttcgccgac
     2221 aacatgaagc ccatgtggga tcaaggtgta ttcaaggtcg agcaactgcg ccgcaagctg
     2281 ttcggggtct gcatggagca
//
LOCUS       DASWQN010000875         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_84209, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000875 DASWQN010000000
VERSION     DASWQN010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84209"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..>3017
                     /locus_tag="VGC82_14765"
     CDS             350..>3017
                     /locus_tag="VGC82_14765"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY0424578.1"
                     /translation="MPDVSAAPPSSALAQELLDLTARIGGRADEDPFGNPVLLVSLAI
                     TRQMDSGDLTETDVAGLIRTLRDAAFADRAGRVASYLGGIDTAVNDGILAGLAQTLLR
                     PDPNDSPVRWAEYRTMTERTRFAAVFTAHPTFSLPLAVNHALAEAACGRPVASFESHR
                     PPPITLRDEFDQAASAIANGRDAIDRFNAALISVARGAWADRWTELTPSPVILSTWVG
                     YDTDGRTDIGWWDTLRLRLEMKRLQLGRLHAQVSVLPSAGALAERILQAGDAVDRQIA
                     LCPDQPEPAKVAAFAHAMVGERETALTSPAPLLPLFRAAIDRAQDHEKLALTVARAGL
                     MSHGMALAHTHTRLNAAQIHNVVRQRLGIADPPEDPSRRRALFGAINAALDTVQPELV
                     DFGALLSEQASAAKLMMTVAQIVKHIDGSVPVRFLIAETETGYTLLAALWLARLFGIE
                     QHIEISPLFETAEALEQGAVVLEEALRSPHYRAYLKRTGRLALQFGYSDSGRYVGQLA
                     ASYLIERLRLKIGQTLEHHGVTGVEVILFDTHGESIGRGAHPGSLADRLKYLSPTASR
                     QALGRAGLKVREEAAFQGGDGYLLFGTPELALATITRIAEQTYHPAAGPIEDPVYADP
                     DFAADFFATIQSNMEGLVEDAGYAALLGAFGPSLLDHTGSRPAARQSDGMGGAARIRH
                     PRELRAIPNNAILQQLGWCANTLQGIGAAAARHPETFTELRQASRRFKRAMDLASHGL
                     AHSDIGVLRAVIATLDPGTWLDRASHTRRPGRREALVSVARALERLDIWAAAQSMFRR
                     ISADHLALRVTWPDVPHMANREVLLHALRLALIHRIWLLATEIPDFSPRHGVTRLAME
                     AAILRLDIETSLNLLAEIFPSATDPAADFDYGE"
BASE COUNT          484 a          998 c         1013 g          522 t
ORIGIN      
        1 ggtcgcgcgt ctggcggcag cacaggcggc tttgggtacg gcatccggcc ggacggctta
       61 agcgcgatcc ggttgctgat aacggcctga gcctggcgcc cttcacgatc actttggaag
      121 cactgaggcg gcgtcggact ggcataccca ccagcgaagc gcgttggtgt tgccgccccc
      181 gatggtccgc accttcgaag tgcaaggatc gcgcttgtcc gggacgatat cgaacagcga
      241 acaccagtga tgccggtggt gggcgcggga gccacggttc gcgtatccgc tgaccggagc
      301 cgccaaatcc gatcttgctg cggcgcacaa atcccgccac actcgcttca tgccggatgt
      361 ttcagctgcc ccgccaagtt ctgctctggc ccaggagctt ctggatctga ccgcgcgcat
      421 cggcgggcgc gccgatgaag atccgttcgg caacccggtc ctgctggtgt cactggcgat
      481 tacccgccaa atggattccg gcgacctgac cgagaccgac gtcgctggct tgatccgcac
      541 cctgcgcgat gccgcgtttg ccgatcgcgc cgggcgcgtt gcctcctacc ttggaggcat
      601 cgataccgcc gtcaatgacg gcattctggc cggtctcgca caaaccctgc tgcgtcccga
      661 tccgaacgac agcccggttc gctgggccga gtaccgaacg atgaccgaac gcacgcgttt
      721 cgcagcggtc ttcaccgcgc atccgacgtt ttccctgccg ctcgcggtca accatgcatt
      781 ggcggaggca gcctgtggcc ggcccgtggc gagtttcgag tcccatcgcc cgccgccgat
      841 cacgctacgc gacgagttcg accaggctgc gtccgccatc gccaacggcc gcgatgccat
      901 cgaccggttc aacgcagcgc ttatctccgt cgctcgtggc gcctgggctg accgatggac
      961 cgaactcacg cccagcccgg tgatcctgtc cacctgggtc ggctacgaca cggacggccg
     1021 caccgatatc ggctggtggg acacgctgcg gctgcggctt gagatgaagc ggctgcagtt
     1081 ggggcgcctg catgcccagg tctcggtgct gccgagcgcc ggcgcgctgg cggaacggat
     1141 cctgcaggcg ggggatgcgg tggaccggca gatcgcgctg tgtcccgatc aaccggagcc
     1201 agcgaaggtc gccgccttcg cccatgccat ggtgggggag cgggaaaccg cgttgacatc
     1261 gcccgcgccg ctgctgccgc tgtttcgtgc ggctatcgac cgtgctcagg atcacgagaa
     1321 actcgcgctg accgtggcgc gcgccgggct gatgtcgcac ggcatggcgc tggcgcacac
     1381 tcataccagg ctgaatgcgg cgcagatcca caacgtcgtg cgtcaacggc tgggtatcgc
     1441 cgatccaccg gaagatccat cccggcggcg cgccttgttc ggcgcgatca atgccgcctt
     1501 ggacacggtg cagcctgaat tggtcgattt cggcgcgttg ctcagcgagc aggcatcggc
     1561 ggcgaaactg atgatgacgg tggcgcaaat cgtcaagcac atcgatggtt cggtgccggt
     1621 ccgtttcctg atcgccgaaa ccgagactgg ctatacactg ctcgccgcgc tgtggctcgc
     1681 ccggctgttc ggcatagagc agcacatcga gatttccccg ttgtttgaga cggcggaggc
     1741 gctggagcag ggcgccgtcg tcctggagga ggcgctgcgc agcccgcact accgggcgta
     1801 tctgaagcgc acgggcagac tggcgctgca gttcggttat tcggactccg ggcgctatgt
     1861 agggcagctc gcggccagct acctgatcga acggttgcgg ctgaagatcg gccagacgct
     1921 ggagcatcac ggcgtgaccg gcgtcgaggt catcctgttc gacacccacg gcgaaagcat
     1981 cgggcgcggc gcgcatcccg gatcgttggc cgaccggctg aaataccttt cgccaactgc
     2041 gtcccgccag gcgctcgggc gggcgggact gaaagtgcgg gaggaagcgg cgttccaggg
     2101 cggcgacggc tacctgctgt tcggcacccc ggaactggcg ctggcgacga tcacccgcat
     2161 cgccgaacag acctaccatc ccgcggcggg tccgatcgaa gatccggttt acgccgatcc
     2221 ggatttcgcc gccgacttct tcgccacgat ccagtccaac atggaaggac tggtggagga
     2281 tgccggttac gcggccctgc tgggtgcctt cggcccgtca ttacttgacc acacgggatc
     2341 gcggccggcc gccaggcagt ccgacgggat gggcggggcc gcccgcatcc gtcatccccg
     2401 ggaattgcgc gcgatcccga acaacgcgat cctgcaacaa ctcggctggt gcgcgaacac
     2461 gctgcaaggc atcggggccg ctgctgcccg ccatccggaa acgttcaccg aactgcggca
     2521 ggccagccgg cggttcaagc gggccatgga tctggcgagc catgggctgg cacactcgga
     2581 catcggggtg ttgcgcgcgg tgatcgccac gctggaccct ggcacctggc tggatcgcgc
     2641 atcccataca cggcgtcccg gccggcgcga ggcgctggtc tcggtcgctc gggcgctgga
     2701 acggctggat atctgggcgg cggcgcagtc gatgttccgg cggatttcgg ccgatcacct
     2761 ggcgttgcgc gtgacctggc cggacgtgcc gcacatggcg aaccgcgagg ttcttctgca
     2821 cgcgctacgt ttggcgttga tccaccgaat ctggctgctg gcgacggaaa ttccagattt
     2881 ctcgccccgt catggggtaa cgcggctggc gatggaggcg gcaatcctgc ggctggacat
     2941 cgagacgtcg ctgaacctgc tggcggagat attcccgtca gcgaccgacc ccgcggcgga
     3001 cttcgattac ggggagc
//
LOCUS       DASWQN010000876         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1271901, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000876 DASWQN010000000
VERSION     DASWQN010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271901"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VGC82_14770"
     CDS             <1..1126
                     /locus_tag="VGC82_14770"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0424579.1"
                     /translation="DSESRFRALGATVLRGEAKFIGLATISVDGRAITARRFVIAAGS
                     RATVPPIDGLDRVAYWTNHSLFDLAEKPDHLLILGGGPIGLEMADAFSGLGCRVTLVE
                     AVRIAAKDDPELVAGLRQALTARGVVFREGVKVIGVSPGPVLLLADGSRVQGSHLLVA
                     VGRTPNLAALNLAAANVKAGPAGVATDQGLRSVSNKRIFAVGDIADPKGIGPRAFTHV
                     GSYHASIVIRRVLFRLPARIDYAALPRVTYTAPELAQAGLTEAEAIASGLKVRILRWP
                     LADNDRAIAERDTVGLVKLIVSGSRVVGAGILAPNAGEMISQWTLAIAQRTKLSALAS
                     MIVPYPTRSEAAKRAAGSFFAASLFAARTKTLVRLLSRLP"
     gene            complement(1186..>1682)
                     /locus_tag="VGC82_14775"
     CDS             complement(1186..>1682)
                     /locus_tag="VGC82_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424580.1"
                     /translation="SSYSGIGTGGQIGRQPFQAQASRAREMSQLEDDSRLSRMLLKVA
                     ENLDAEADAMEAEGRERRASARIRQIEMFGTLSSASGPQEALVQITNLSAGGAKIRFG
                     GNGEPPAGNVVLAVPNLGLFLKGTILRTRGAEAAIIFDSTTSADPCLAWVLQSEPMMA
                     DGTV"
BASE COUNT          285 a          528 c          536 g          333 t
ORIGIN      
        1 ggactcagag tcgcggttcc gcgctctggg ggcgacggtg ctgcgtggcg aagccaaatt
       61 cattggtctg gcgacgattt cggtggacgg gcgcgccatc accgcgcgcc ggtttgtcat
      121 cgcggccggc agccgcgcga ccgtgccgcc gatcgacggc ctggatcgag tcgcatattg
      181 gaccaaccac tcgctcttcg atctggcaga gaagccggac catttgctga tccttggcgg
      241 cggaccgatc ggattggaga tggcggatgc gttctccggc ctcggctgtc gggtcaccct
      301 cgtggaggca gtgcggatcg cggcgaagga cgatccggag ctggtggccg gcctgcgtca
      361 ggcgctgacc gctcgcgggg tggtgttccg cgaaggtgtc aaggtcatcg gcgtttcgcc
      421 cggaccggtg ttgctgctgg cggacggatc ccgggtgcaa ggctcgcact tgctggtggc
      481 ggtggggcgg acgcccaacc tggcggcgct caatctagcg gcggccaatg tcaaggcggg
      541 accggcaggc gtcgcaacgg accaaggcct gcgcagcgtc agtaacaagc gcattttcgc
      601 ggtcggagat atcgccgacc cgaaggggat cggaccgcgg gccttcaccc atgtcgggtc
      661 ctatcatgcc agcatcgtca ttcgccgcgt gctgttccgg ctgccggcac ggatcgatta
      721 tgccgctctg cctcgggtta cctacaccgc tccggaactg gcgcaggccg ggctgacgga
      781 agccgaggcg atcgcttccg gtttgaaggt gcggattcta cgctggccgc tggcggacaa
      841 cgatcgcgcc atcgccgaac gggacaccgt cgggctggtc aagctgatcg tcagcggaag
      901 tcgggtggtg ggggccggta tcttggcccc aaacgctggc gagatgatca gtcaatggac
      961 cctcgccatc gcccaacgca cgaaactgtc ggcgcttgcc agcatgatcg tgccatatcc
     1021 aactcgttcc gaagcggcca agcgcgcggc tggcagtttt ttcgccgcat cccttttcgc
     1081 ggctcggacc aagacgctgg tgcgcctgct gtcccgattg ccctaaccgt atgttgccct
     1141 agccgtatat tgcatcgcgg cacattctat cgcggcgtct tctgttcaaa ccgttccgtc
     1201 cgccatcatc ggctcgcttt gaagaaccca cgccaggcag gggtcggcgc tcgtggtgga
     1261 atcaaaaata atcgcggctt cggcgccacg cgtcctcagg atcgtccctt tcaggaaaag
     1321 gccgaggttc ggcaccgcca gtaccacgtt gcccgccggt ggctccccgt tgccaccgaa
     1381 ccggatcttt gcaccgccgg ccgaaagatt ggtaatttga accaacgcct cttgtggccc
     1441 ggacgcactt gacagggttc caaacatctc aatctgacga atacgagcag aggcgcggcg
     1501 ctcccgccct tcggcttcca tcgcgtcggc ttcggcatcg agattttctg caactttcag
     1561 caacattcgt gaaagccgag aatcgtcttc caattgagac atctcgcgcg cgcgggatgc
     1621 ttgtgcctga aatggctggc gtccaatctg acctcctgtt ccaattccag aataggatga
     1681 tc
//
LOCUS       DASWQN010000877        12031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1007428, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000877 DASWQN010000000
VERSION     DASWQN010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 12031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12031
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007428"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="VGC82_14780"
     CDS             <1..1522
                     /locus_tag="VGC82_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424581.1"
                     /translation="AQSGRVLKFIPQSDVTVLDPIWTTAYVTRNHGYMIFDTLFGTDG
                     AFKPSPQMAAGLLTENDGKLVRIPLREGLKFHDGSPVLARDCVASIQRWAKRDTFGQT
                     LMAATDELTASDDKTIQFRLKRPFAMLAGALGKSPTNFPAMMPERLAKTDAFTQVTEM
                     VGSGPYRFNTRERVVGSMAVYDRFADYVPRTDGTPNWTAGPKVAHFDRVEWHVIPDAG
                     TALAALQTGEVDWWENPGNDLLPALRQAAGVKVEVQDPTGLMGCMRLNHLAAPFDNPN
                     IRRALLKAIDQRDFCLASSGDDPAMWHVPTGLFCPQSPFATPAGLEVFSGKRDYDAVR
                     KEIVAAGYKGEKTVVLAPTDFPLLKAMADVCADVFTKIGLNVDYQSMDWGTVVQRRIK
                     KDPTDKGGWSVFNTFWAGLDQFNPVGHVFMRGIGDSPGSSPGWPSSPRIEQLRQQWLE
                     APDLAAQQSLARDLQLQALTDVPYVPLGQVLGATAYRTNLTGVLNGFVLFWNVRKT"
     gene            complement(1583..2509)
                     /locus_tag="VGC82_14785"
     CDS             complement(1583..2509)
                     /locus_tag="VGC82_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0424582.1"
                     /translation="MTAVATAVHRPGESRGLARLAGQRPRRAARRRRYFTNVAALLLS
                     AVILVWTITPIYNMVMVAFEAEGDVFSEHIWPPHPSPRSFWIVITQGYWYLEHFWHQF
                     GNSAYIAGMTTLLVLLIGSMTSFSVGRMRIKNGWLVTNAALLTYVIPASFLAIPFYLI
                     MNSYGLHDNPWSVIAALVTFATPYAIFIFQEYGRSIPIELDESARIDGASPLQIYARI
                     YLPLMAPALVAVGTYALLLAWNEYLYQFLLLSNKESMTVVVALAQFINSDQAPWNYMM
                     ATALIYALPPVATYYAFRRRMSSGLTMGGVKG"
     gene            complement(2506..3420)
                     /locus_tag="VGC82_14790"
     CDS             complement(2506..3420)
                     /locus_tag="VGC82_14790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY0424583.1"
                     /translation="MNTALAIDHGRPVVRRSSVLRYWQGRLRGSEFTWAVAFVVPYIG
                     VFLAFVVYPVLYGAWMGSDPHLYTQLFSDPIYLQTVANTLIFLAVGVNIKLFLALLLS
                     GFFMRPGWLMKGLLLIFVLPWAIPALPTFISIHWMLNGEWGLLNNALYILFGIEGPSW
                     LNERWLALGSAICAHIWKWLPFWTVILLAGRMSIPQDIYDAAKVDGATGLRSFMHVTF
                     PMLANLYLVLTLLATIFLLGDFNSVTFVTGGGPANSTHVLATLGIRNAYDMGHPELGV
                     AAVMSALPLMIPLVIILMRKLRTSDVQL"
     gene            complement(3430..4818)
                     /locus_tag="VGC82_14795"
     CDS             complement(3430..4818)
                     /locus_tag="VGC82_14795"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424584.1"
                     /translation="MANLGGSLGRRSLLRGSVGLGAAGALARPHIAKAAATTATIWWI
                     QGFIQEEDVAFRELVAEYQKVSGNTLDYSIIPYAPMRQKIVSAVTSGLVPDLFQNTPT
                     ELIALYAWDDKLLDLSDVVDTQRAEYTETALLSAFCYNSVKKTRGYYGVPYTTAVLPN
                     HIWRPLVEKAGYKIEDIPKTWDAYYDFFKNVQKKLRAQGERKVYGLGFQVTTNGNDPN
                     NVFNYFLLANGGQDLVTKDGKLHLDDPKVREGVIKALTYPATAYKEGFVPPGAINWND
                     ADDNNAFHAKQVVMDLDGTISTEVAVIHNEQDYRDIVTMGLPLGNDGKPVPSQAANAF
                     GLIPKGAKNVEVAKDFLKYLIQPKVTNAYLKTGLGRSIPAMLSIVKNDPWWLDPADPH
                     RVAYVQQGLFAPTSPSFWAYNPAYAQVQNEHVWSAAWIDIMTGGMTPQAAADKAFKRV
                     EEIFAKYPIVQS"
     gene            5110..6687
                     /gene="secD"
                     /locus_tag="VGC82_14800"
     CDS             5110..6687
                     /gene="secD"
                     /locus_tag="VGC82_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY0424585.1"
                     /translation="MMYFSRMKATLILGVCLLGMLLAVPNLFAAPAAWMPWRTVHLGL
                     DLRGGSYLLLEVDMKAVLKERLESLADGVRQALRGGQIFYQTLEAQPDQTRVLLRLRD
                     ASKTDAAVAALRPLISPDASTGLPDMAIGSDPSGTVTLTLSATALAARATGAVDQSIE
                     IVRRRIDETGVVDPQITRQGQSRIVVQLPGVGDPNRIKQLIGKTAHMTFRLVDETANA
                     ASGGPPPPGDDFLQMQDRPGEKIGVRKRIDVDGADLTDARAGTSPETGDWVVNFTFNS
                     VGARRFADVSRANVNHRFAIVLDDKVISAPVIREAITAGRGQISGSFNASSANDLAVL
                     LRAGALPAPLIVVEERSVGPELGADSIRAGAVSLVAGFVFVIAFMGFFYGLFGWFANL
                     ALVVNLLLMLGVMSLLQATLTLPGMAGILLTLGMAVDANILINERIREEQKLGRPPLA
                     ALEHGFSRAYSTIFDSNATAFLAHVMLFVFGSGPVKGFAVTITVGIATSLFTAWMLTR
                     LLVSRWYIATRPKLLPV"
     gene            6754..7716
                     /gene="secF"
                     /locus_tag="VGC82_14805"
     CDS             6754..7716
                     /gene="secF"
                     /locus_tag="VGC82_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY0424586.1"
                     /translation="MFIKPRFRLVPDNTRINFMRGRYMGLIVSAILSTASVIMFFYPG
                     LNLGIDFSGGVVMEVRTDGPADFSQIHAALSAEHIPEQGVQRFGDASEVLIRLGAQSS
                     EQATQAAVARVRTALEKGVPGARIQRTDAVGASVSAELFRNGLLALGISLLMILVYIW
                     FRFEWQFAVGAVVTLVLDITKAIGFLALTRIEFDLVMVAAILTVLGYSTNDKVVVYDR
                     VRENLRKYKSMPLRELIDLSINETLNRTLGTSMTVFLASLPLALFGGESISGFAWVML
                     FGIVVGTSSSIFIAAPILLFLGEHRLRRDSAAPLVAKAAPASRP"
     gene            7713..8705
                     /locus_tag="VGC82_14810"
     CDS             7713..8705
                     /locus_tag="VGC82_14810"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY0424587.1"
                     /translation="MTIGRRRLLTGLPVATALALAPVAAGAVQPIQPRRHLTMLLGFA
                     AGTGADRIARDFAGFLAQHLVGADITMNNLPGGAGRSGLAALGDAAANGATIGWVVTP
                     TLPARVIDLNEPSLGQRIQLLGQVEREPIAFVSPAKDPANSIQDIIQRASDDADAVPL
                     GTPPAGSPPHLAALRLQVLAQTQLNIVTFPSAAAARQAVLAGNVSAAALGLAEAIGDI
                     RDGNLVAIGIAAKRRFGLLPDTPVLDEAGIPLSAFIRRGIAVPVGTPAEAVIPVVEAM
                     RAAAMDEAFREQAEAKGYYAAWAEGPAWAAQMQTEQEALAKLWETNPWLSSSGG"
     gene            8909..9904
                     /gene="glpX"
                     /locus_tag="VGC82_14815"
     CDS             8909..9904
                     /gene="glpX"
                     /locus_tag="VGC82_14815"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEY0424588.1"
                     /translation="MASNDKPLPGTMTDRNLALELVRVTEAAALAASRWIGHGKKNEA
                     DGAAVEAMRKAFDAVAIDGTVVIGEGEMDEAPMLFIGEKVGAGGPAMDIAVDPLEGTT
                     LTAKGGPNAMATVALAESGNFLHAPDIYMDKIAVGGGLPDGVVTLDDSVGNNLRNLAR
                     AKKRDVSELVACILDRDRHKELIAYCREAGARVMLISDGDVAGVIATAMPDAEIDIYL
                     GSGGAPEGVLAAAALRCVGGQMQGRLLYESDEQIERVRSMGHPDPKRCFSVEEMAKGD
                     VMFAATGVTAGAMLRGVSRRPGGATTHSIVMRSHSGTVRFIEAHHNFVQKTWTPT"
     gene            9930..11708
                     /gene="recJ"
                     /locus_tag="VGC82_14820"
     CDS             9930..11708
                     /gene="recJ"
                     /locus_tag="VGC82_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY0424589.1"
                     /translation="MTPAETVLGVTRSLSGRRWLWRTGEERVAAGIAQRLVLPEIVSR
                     LLAARGIGIDAAADFLEPTLRVMLPDPSTMADMDIAADRLADAVRRGETVGVYGDYDV
                     DGACSAALMVGFLRELGCPVHHHVPDRIKEGYGPNAPALVALAARGASLIVCVDCGTA
                     AAVVLASVAGRAEVIVLDHHKAEGMPPAILATVNPNRLDDGSGLTHLCAAGVAFLTAI
                     ATVRALRRLGFFAARPEPDLMGLLDLVALATVCDVMPLTGVNRAFVCQGLKVMARRSR
                     AGIAALLDITQAREKLNASTCGYALGPRINAGGRISEADLGLRLLLCEDRIEALALAE
                     KLEAVNRMRQDVEAGILETAMFAAELQVAAGRAAVLVTGEGWHPGVVGIVAGRIKERF
                     NRPACVAGFDGGLGKGSGRSVPGLDLGAAVIAARQAGLLVTGGGHAMAAGFSVRSERL
                     DAFHAFLDERLAAAAAMPGAADLVVEGTLAVAGATTEVARHLERLAPFGAGNEEPTLV
                     LHRARVVRADRVGREANSIRAFVEGEGGAGRLKAMMFRAREGAVADALLARADCRPLH
                     LAGHLRAEEWNGSVSPCFVISDAAMA"
     gene            11797..11872
                     /locus_tag="VGC82_14825"
     tRNA            11797..11872
                     /locus_tag="VGC82_14825"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11831..11833,aa:Glu,seq:ttc)
BASE COUNT         2072 a         3608 c         4101 g         2250 t
ORIGIN      
        1 ggcgcagtcc ggccgcgtgc tgaaattcat tcctcaatcg gacgtaacgg tgctcgatcc
       61 gatctggacc acggcctatg tcacccgcaa ccacggctac atgatcttcg acacgctgtt
      121 cggcaccgac ggcgcattca agcccagccc gcaaatggcg gcggggctgc taaccgagaa
      181 tgacggcaaa ctggttcgta tcccgctgcg cgagggcctg aagttccatg acggctcgcc
      241 ggtgctggcg cgggactgcg ttgccagcat tcagcgctgg gcgaagcgcg acacgttcgg
      301 tcagaccctg atggcggcca cggacgagct gaccgcgagc gacgacaaga ccatccagtt
      361 tcgtctcaaa cgcccattcg cgatgctggc gggcgccctg ggcaaatccc caaccaattt
      421 tccggcgatg atgccggagc ggctggcgaa gaccgatgcg ttcacgcagg tcaccgaaat
      481 ggtcggcagc ggtccttatc gcttcaacac gcgggagcgc gtggtcggct cgatggcggt
      541 gtatgatcgg ttcgccgact acgttccaag gaccgatggc acgccgaatt ggaccgccgg
      601 cccaaaggtg gcgcatttcg accgggtcga gtggcacgtc attcccgacg ccggcaccgc
      661 gttggcggcg ttgcagaccg gtgaggtcga ttggtgggaa aacccaggca acgacctatt
      721 gcccgcgttg cgccaggcgg ccggtgtcaa ggtcgaggtg caggatccga cgggactgat
      781 ggggtgcatg cggcttaatc atttggccgc tccgttcgac aatccgaaca tccggcgggc
      841 gctgctgaag gcgatcgacc agcgcgattt ctgccttgcc agcagtggcg acgatccggc
      901 gatgtggcat gtgccgaccg ggctgttctg tccgcaaagc ccgttcgcga caccggccgg
      961 actggaggtg ttcagcggca agcgcgacta cgatgccgtg cgcaaagaaa tcgtcgcagc
     1021 cggctacaag ggagaaaaga cggtcgtgct ggcaccgacc gattttccgc tgctgaaggc
     1081 gatggccgat gtctgcgccg acgtgttcac caagatcgga ctgaacgtcg attaccagtc
     1141 gatggactgg ggcaccgtgg tgcagcgtag gatcaagaag gacccgaccg ataagggcgg
     1201 ctggagcgtg ttcaacacgt tctgggctgg gctcgatcag ttcaacccgg tcggacatgt
     1261 gttcatgcgc ggcattggcg acagtcccgg ttcttccccc ggatggccat ccagcccgcg
     1321 catcgagcaa ctacggcagc aatggctcga ggcgccggat ttggcggcgc aacaatcgct
     1381 ggcgcgggat ctccagcttc aggcgcttac cgacgtgccc tacgtgcctt tggggcaggt
     1441 actgggggcg acggcctatc gcaccaacct gaccggagtg ctgaacgggt tcgtgctgtt
     1501 ctggaacgtt cgcaagacct gactgcgcgg cagattggcc ttgaacctcc gatggctggc
     1561 cttgaacctc cgatggccac cgtcagcctt tcacgccacc catggtcagg ccagaggaca
     1621 tccggcggcg gaatgcgtag taggtcgcga ccggcggcag agcgtagatc agggcggttg
     1681 ccatcatata gttccacggc gcctggtcgc tgttgatgaa ctgagccagg gccaccacca
     1741 cggtcatgct ttccttgttc gacagcaaca ggaactgata cagatattcg ttccacgcca
     1801 acagtaacgc gtaggtgccg accgcgacca gtgccggcgc catcagcggc aaatagatgc
     1861 gggcgtagat ctggagcggc gatgcgccgt ctattcgcgc cgattcgtcg agttcgatcg
     1921 ggatgcttcg gccgtactcc tggaagatga agatcgcata gggtgtggca aaggtcacca
     1981 gcgctgcgat caccgaccac ggattgtcgt gcaacccgta gctgttcatg atcagataga
     2041 atgggatggc caggaacgat gccgggatca cgtaggtcaa caacgcggcg ttggtgacca
     2101 gccagccgtt cttgatacgc atccgtccca cggagaaact ggtcatggac ccgatcagca
     2161 gcaccagcag cgttgtcatg ccggcgatat aggcgctgtt gccgaactgg tgccagaaat
     2221 gctccagata ccagtagccc tgggtgatga cgatccagaa actgcggggc gacgggtgcg
     2281 gcggccagat gtgctcgctg aagacgtcgc cttcggcctc gaatgcgacc atgaccatgt
     2341 tgtagatcgg tgtgatcgtc cagaccagga tcacggcgct gagcagcagg gccgccacat
     2401 tggtaaaata gcgtctccgc cgcgcggcgc ggcgcggtcg ctgccctgcg agccgcgcca
     2461 ggcctcggct ttcccctggc cgatgaacgg cggttgccac ggcggtcaca gctgtacgtc
     2521 cgaggtgcgc agcttgcgca tcagtatgat caccagcggg atcatcaacg gcagcgccga
     2581 catgacggcg gccacaccga gctccggatg gcccatatcg taagcgttgc ggatgccgag
     2641 cgtcgccagc acatgggtgg agttggccgg gccgccgccg gtgacgaagg tgacggagtt
     2701 gaagtccccg agaaggaaga ttgtcgccag cagggtcaga accaaataaa gattggcaag
     2761 cattgggaac gtcacgtgca tgaagctgcg caaaccggtt gcaccgtcca ccttggccgc
     2821 gtcgtagatg tcttgtggga tcgacatccg gcccgcaagc aggatcaccg tccagaacgg
     2881 cagccacttc cagatatggg cgcagatcgc cgaacccagg gcgagccagc gttcattcag
     2941 ccaggacggg ccctcgattc cgaagagaat atacaacgcg ttgttcagca ggccccactc
     3001 gccgttcagc atccagtgga tggagatgaa cgtgggcagt gccgggatcg cccagggcag
     3061 gacgaagatc agcaacagtc ccttcatcag ccagcccggg cgcatgaaga agccggacag
     3121 tagaagcgcc aaaaacaact tgatgttcac ccctacggcc agaaagatca gcgtgttggc
     3181 cacggtttgc aggtagatcg ggtcggagaa tagctgagtg tacaggtgcg gatcgctgcc
     3241 catccacgct ccgtaaagca ccggatagac cacgaaagcc aggaacaccc cgatataggg
     3301 cacgacgaag gcgacggccc aggtgaactc ggagcctcgc aggcggcctt gccaataacg
     3361 caggacggac gaccgacgga caaccggacg gccgtgatcg atagccagag cggtattcat
     3421 gatcgcgcct cagctttgca cgatgggata tttcgcgaag atctcctcga cacgcttgaa
     3481 cgccttgtcc gccgccgcct gcggcgtcat tcccccggtc atgatgtcga tccaggcggc
     3541 gctccagaca tgctcgtttt ggacctgggc ataggccggg ttgtaggccc agaacgatgg
     3601 tgaggtcggg gcgaacaatc cttgctggac gtaggcgacc cggtgcggat cggccgggtc
     3661 gagccaccat gggtcgttct taacgatcga cagcatcgcc ggaatgctgc gtcccaggcc
     3721 cgtcttcagg tatgcattgg tgaccttcgg ctggatcaag tatttcagga agtccttggc
     3781 cacttcgacg ttctttgcgc ccttcgggat caggccgaag gcattggcgg cttggctcgg
     3841 caccggcttg ccgtcgttac ccagcggcag gcccatcgtg acgatgtcgc ggtagtcctg
     3901 ctcgttgtgg atgaccgcga cctcagtcga gatcgtgccg tcgaggtcca tgaccacctg
     3961 cttggcgtgg aaggcattgt tgtcgtcggc gtcgttccag ttaattgccc ccggcggtac
     4021 aaagccttcc ttataagcgg tagccggata ggtcagtgcc tttatgacgc cttcgcgcac
     4081 ctttgggtcg tcgaggtgca gcttgccgtc cttggtgacg agatcctggc cgccattggc
     4141 aagcaggaaa tagttgaaca cgttgttggg atcattgcca ttggtggtga cctggaaacc
     4201 aaggccataa accttgcgct ctccttgcgc gcgcaatttc ttctgcacat tcttgaagaa
     4261 gtcgtagtag gcatcccagg tcttcgggat atcctcgatt ttgtagccag ccttttccac
     4321 caacggccgc cagatatggt tcggcagcac tgctgtcgtg taaggcacgc cataatagcc
     4381 gcgcgttttc ttgacgctat tgtagcagaa ggctgaaagc agcgcggttt cggtatattc
     4441 cgcccgttgc gtgtccacca cgtcggacag gtcgagtagt ttatcatccc acgcatacag
     4501 cgcgatgagc tcggtagggg tattctggaa caggtcgggg acgagcccgc tggtaacagc
     4561 ggaaacgatc ttctggcgca tcggcgcgta ggggatgata ctgtaatcca gggtattgcc
     4621 gctgaccttc tggtattcgg ccaccagttc gcggaatgcc acatcctctt cctggataaa
     4681 tccctgtatc caccaaatgg ttgccgtggt ggcggccgcc ttggcgatat gcgggcgcgc
     4741 cagggcgccg gctgccccta atcccaccga tccacgaagc aatgagcgtc ggccaaggct
     4801 gcccccaagg ttggccatgt cctttatcct tccctgcttt tatcgcgcta acccttgttc
     4861 gggtgcggta tgatttttca gcagacgtta ggcccaagag cgtcgtgtcc gcaaggccgc
     4921 cgtttggtcc gcactcgttc gtgcggcagg tcaaccgggc gacctggccg atgcgcgacc
     4981 ggcgagatca gcaggccgat tcttttccgg tgcgggcaac cgaacactgg acaatccgct
     5041 cggcggcagc gcattaggat cgccgccgaa cgaacggcac ggtgtcccgg gagctccgcg
     5101 cgtaagacca tgatgtattt cagccgcatg aaagccacgc tcatcctggg cgtctgcctg
     5161 cttggcatgc tcctggcggt gccgaacctg tttgccgcgc cggcggcctg gatgccctgg
     5221 cggaccgtgc atctcggcct cgacctgcgc ggcggcagtt acctgctgct ggaagttgat
     5281 atgaaggcgg tcctgaagga acggctggaa agcttggccg atggggtgcg ccaggcgctg
     5341 cgcggcgggc agattttcta tcagacgctg gaagctcagc cggaccagac cagggtgctg
     5401 ctgcgcctgc gcgatgcgtc caagaccgac gccgcggtcg cggccctgcg gccattgatc
     5461 agcccggacg cgtccaccgg gctgccggac atggcaatcg gctccgatcc cagcggaacc
     5521 gtcacgctca cgctgtcggc cacggcgctg gcggcgcggg ccaccggagc cgttgaccag
     5581 tcgatcgaga tcgtgcggcg cagaattgac gaaaccggtg tggtggatcc gcagatcacg
     5641 cgccagggac agagccgcat cgtcgtgcaa cttcccggtg tcggcgatcc gaaccggatc
     5701 aagcagttga ttggcaagac cgcccacatg accttccggc tggtggacga gaccgccaat
     5761 gcggcgtcgg gcggcccgcc gcctccaggc gacgacttcc tgcagatgca ggatcgcccg
     5821 ggagagaaga tcggcgtgcg caagcgaatc gatgtcgatg gcgcggatct gacggatgcc
     5881 cgcgccggca ccagccccga aaccggggac tgggtggtga acttcacctt caactcggtg
     5941 ggcgcacggc gtttcgcgga tgtgtcccgg gcgaatgtga accaccggtt tgccatcgtg
     6001 ctggacgaca aggtgatttc cgcaccggtg atccgcgagg cgatcaccgc cggccgcggg
     6061 cagatcagcg gctcgttcaa cgcgagttcg gccaatgatc tggcggttct attgcgggcc
     6121 ggcgccttgc cggcgccgct gatcgtggtc gaggaacgtt ctgtcggacc cgaactgggt
     6181 gcggacagca ttcgggcggg ggcagtgtcc ctcgttgccg ggtttgtgtt cgtgatcgcc
     6241 ttcatgggct ttttctatgg actgttcggc tggttcgcga acctggccct ggtggtcaac
     6301 ctgctgttga tgctgggcgt catgtcgctg ctgcaggcga cgctgacgct tccgggcatg
     6361 gccggcatcc ttttgacgct gggcatggcg gtcgatgcca acatcctgat caacgagcgg
     6421 atccgcgagg aacaaaagct gggacgtccc cccttggccg cgctggagca tggattttcc
     6481 cgcgcctaca gcaccatctt cgacagcaac gccacggcgt tcctggccca tgtgatgctg
     6541 ttcgtcttcg gttcgggacc ggtcaagggt ttcgccgtca ccatcaccgt cggtattgcc
     6601 acgtcgctgt tcaccgcgtg gatgctgacc cggcttctgg tctcccgctg gtacatcgcc
     6661 acgcggccga agttgctgcc ggtttagttc gatcgcttga ttcgcgtggt ttgggcgtcc
     6721 gcccggcgcg atcacgctct gaggtttctg cccatgttca tcaagccccg cttccgtctg
     6781 gttcccgaca acacccgcat caacttcatg cgcgggcgct acatggggct gatcgtttcg
     6841 gcgatcctgt ccaccgcctc ggtcatcatg ttcttctatc ctggtctgaa cctgggcatc
     6901 gacttttccg gcggcgtggt gatggaggtc cgcaccgacg gtcccgccga tttttcccaa
     6961 attcatgccg cgctcagtgc cgaacatatc ccggagcaag gggtgcagcg gtttggcgac
     7021 gcgtcggagg tgctgatccg gctgggcgcg caatcgtccg aacaagcgac gcaggccgcg
     7081 gtcgcccggg tgcggacggc gttggagaaa ggcgtgcccg gcgcccgcat ccagcgcacc
     7141 gatgcggtcg gcgcgagcgt gtcggccgaa ctgttccgca acggtttgct cgcgctgggc
     7201 atcagcctgc tgatgatcct ggtctatatc tggttccgct tcgaatggca gttcgccgtg
     7261 ggggcggtgg tcacgctggt gctggacatc accaaggcga tcggatttct cgcgctgacc
     7321 cggatcgagt tcgatctggt gatggtcgcg gccatcctga cggtgctggg ctattccacc
     7381 aacgacaagg tagtggtgta cgaccgggtc cgggaaaacc ttcgcaaata caaatcgatg
     7441 ccgctgcggg agcttatcga cctgtcgatc aacgagacgt tgaaccggac tcttggcacg
     7501 tcgatgaccg tgttcctggc aagcctgccg ctggcgctgt ttgggggcga gtcgatctcg
     7561 ggcttcgcgt gggtcatgct gttcggcatc gttgtcggta cttcgtcgtc gatcttcatc
     7621 gccgcgccga tcctgctgtt cctgggtgaa catcgcttgc gccgcgactc ggctgcgccg
     7681 ctggtcgcca aggccgcgcc ggcctcgcga ccgtgacgat tggccggcgg agattgctga
     7741 ccggcctgcc ggtcgctacc gcgctggctt tggcgccggt tgcggccggg gcggttcagc
     7801 cgatccagcc caggcggcac ctgacgatgc tgctcgggtt tgccgcggga accggtgcgg
     7861 acaggatcgc ccgcgatttc gccggcttcc tggcacagca tctggtcggc gccgatatta
     7921 caatgaacaa cctgcccggg ggtgccggac gaagcgggct ggccgccctg ggcgatgcgg
     7981 cagccaatgg tgccaccatc ggctgggtgg tgacgcccac gctgccggcg cgcgtcatcg
     8041 atctcaatga gccgtcgctt gggcagcgga tccagttgct cgggcaggtc gaacgcgagc
     8101 cgatcgcttt cgtctcaccg gcgaaagatc cggccaattc gatccaggac atcatccaac
     8161 gcgccagcga cgatgccgat gcggtgccgc ttggcacacc ccccgccggc agtccgccgc
     8221 atctggcggc actgcggctg caggtgctcg cacagacaca actgaacatc gtcacgttcc
     8281 cgtccgccgc ggcggcaagg caggcggtgc tggcgggcaa tgtttcggcc gccgcgctgg
     8341 gactggcgga ggcgatcggc gacatccgcg acggcaacct ggtcgcgatc ggcatcgcgg
     8401 ccaagcgtcg gttcggcctg ttgccggaca ctcccgtgct ggacgaggcc ggcattccgc
     8461 tgtcggcctt tatccgccgc ggcatcgcgg tgccggtggg gacgccggcg gaagcggtca
     8521 taccggtggt cgaagcgatg cgagcggcgg ctatggacga ggcgtttcgc gaacaggcgg
     8581 aggccaaggg ctactatgcg gcctgggcgg aaggtccggc atgggccgca cagatgcaaa
     8641 ccgaacagga agcgctggcg aagctttggg agaccaaccc ttggctgtcc tcgtcgggtg
     8701 ggtgagtcgg gtggatgcgg atactgaccc cggccgcgac gcgccatcat gcccgaatgc
     8761 cggcctgtgc gggcgccggc ttctgtgggc tccgctcgtg tgggctccag gcctgtgcgg
     8821 gcgtcggcct gtgcgggcgt cggcctgtgc ggcgaggcgg tccatgctaa gtagtgcaga
     8881 ctcgaatcgc gtcctgaagg aggaatccat ggccagcaat gacaagccgc tgcccggcac
     8941 gatgaccgac cgcaacctgg cgctggaact ggtccgcgtg acggaagccg cggcgctcgc
     9001 ggcgtcccgc tggatcggcc acggcaagaa gaacgaggcg gatggtgcgg cggtggaagc
     9061 aatgcgcaag gccttcgacg cggtcgccat cgacggtacg gtggtgatcg gcgaaggcga
     9121 gatggacgag gcgccgatgc tgttcatcgg cgaaaaggtc ggcgccggtg gtccggcgat
     9181 ggatatcgcg gtcgatccgc tcgagggcac cacgctgacc gcgaagggtg gcccgaacgc
     9241 aatggctacg gtggccttgg ccgaaagcgg caattttctg catgcccccg acatctacat
     9301 ggacaagatt gcggtaggcg gtggtttgcc ggatggagtg gtaaccctgg acgactcggt
     9361 cggcaacaat ctgcgcaatc tggcgcgggc gaagaagcgc gatgtgtcgg aactggtggc
     9421 ctgcatcctg gaccgcgacc ggcacaagga gctgatcgct tactgccgcg aggcgggtgc
     9481 ccgggtcatg ctgatcagcg atggcgatgt agccggcgtg atcgccaccg cgatgccgga
     9541 tgccgagatc gacatatacc tggggtctgg tggcgcgccg gagggcgtgc ttgcggcggc
     9601 tgcgctgcgc tgcgtcggcg ggcagatgca ggggcggttg ttgtacgaat cggatgagca
     9661 gatcgaacgc gtccgctcga tgggtcatcc cgacccaaaa cgctgttttt cggtggaaga
     9721 gatggccaag ggcgacgtga tgttcgccgc gacgggcgtc accgcgggcg cgatgctgcg
     9781 cggcgtcagc cgccgccccg gtggtgcgac cacccattcg atcgtgatgc gcagccactc
     9841 cggcaccgtg cgtttcatcg aggcgcacca caacttcgtg cagaagacgt ggacgccgac
     9901 ctgatgacga aggtcgggag gaagacgttc tgacgccggc cgaaaccgtc ctcggcgtga
     9961 cgcgaagcct gtccgggcgg cgctggttgt ggcggaccgg ggaggaacgc gtggcggccg
    10021 gcatcgccca gcggttggtg ctgcccgaga tcgtcagccg cctgctggct gcaaggggga
    10081 tcggcatcga cgccgccgcc gatttcctgg aacccacgct gcgggtaatg ctgccggatc
    10141 cctccacgat ggccgacatg gatatcgcgg ccgatcggct ggccgatgcg gttcgccgtg
    10201 gcgaaaccgt tggcgtttat ggcgactacg atgtggacgg ggcctgcagc gccgcgctga
    10261 tggtcggctt cctgcgcgaa ctgggttgtc ccgtgcatca ccatgtgcct gatcgcatca
    10321 aagagggtta tggccccaac gcaccggccc tggtcgcact ggcggcccgt ggtgccagcc
    10381 tgatcgtttg tgtcgattgc ggcaccgccg cggcggttgt gctggcgtcg gtcgccggtc
    10441 gggccgaggt gatcgtgctg gaccatcata aggcggaggg gatgccgcct gcgatcctgg
    10501 ccacggtcaa cccgaaccgc ttggatgatg gttcgggact gacccatctg tgcgccgccg
    10561 gcgtggcgtt cctgaccgcg atcgccaccg tgcgggcgct gcgccggctg ggctttttcg
    10621 ccgcgcggcc cgaacctgac ctgatggggc tgctggatct ggtggcactg gcgacggtat
    10681 gcgacgtaat gccgctgacc ggggttaacc gggcgttcgt gtgccaggga ttgaaggtga
    10741 tggcgcgccg gtcccgggca gggatcgcgg ccttgctgga catcacccag gctcgggaga
    10801 agttgaacgc gtccacctgt gggtatgcgc tgggcccgcg gatcaatgcc ggcgggcgga
    10861 tcagcgaagc cgacctgggg ctgcgcttgc tgctgtgcga ggaccggatc gaggcacttg
    10921 cgctggccga gaagttggag gcggtcaacc gcatgcgcca ggacgtcgag gcgggcatct
    10981 tggaaacggc gatgttcgca gccgagcttc aggtcgcggc cggccgggcg gcggtgttgg
    11041 tgacggggga ggggtggcat cccggggtgg tcggcatcgt cgccggccgg atcaaggagc
    11101 gtttcaaccg cccggcctgc gtggcagggt tcgatggcgg gttgggcaaa ggctctggcc
    11161 gatcggtccc aggcctcgat ctgggagcgg cggtgatcgc ggctcgccag gcgggtctgc
    11221 tggtgaccgg cggcggacat gcgatggcgg ctgggttttc ggtgcgcagt gaacgactgg
    11281 atgcattcca cgcgttcctt gatgagcgcc tggccgcggc agcggcgatg cccggcgctg
    11341 cggatctggt cgtcgagggg accctggcag tcgcgggggc gacgaccgaa gtggcgcggc
    11401 atctggagcg gctggctcct tttggagcag gaaatgagga gccgactttg gtgctgcacc
    11461 gcgcgcgggt cgtgcgtgcc gatcgggtag ggcgcgaggc gaactcgatc cgagcgtttg
    11521 tggagggtga gggcggggcc ggacggttga aggccatgat gttccgggcg cgggaagggg
    11581 cggtggcgga tgcgttgctg gcgcgggctg attgcaggcc gctgcatctc gcggggcatc
    11641 tgcgggcgga ggaatggaat ggttcggtca gcccgtgctt tgtcatttcg gacgccgcga
    11701 tggcgtgaga atgggctgtg atcagggtgc ttgaccgccg atggcgggtc cgctacccac
    11761 cgccggcccg gcggttatcg ctgttggctc ggccttgtcc cgttcgtcta gaggcccagg
    11821 acaccgccct ttcacggcgg taacacgagt tcgaatctcg tacgggacgc caattgaatg
    11881 aacaaattgc tggatgggtg gcggtaattg agcagggttt accaagaacc ttaccaagat
    11941 agtcattact gaatgacggc gaacagggtc ggatatttgc cgctacgcgg cgcgcgtaac
    12001 attcgaacag cgcggaaggc tgcttcgccg g
//
LOCUS       DASWQN010000878         4378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2458714, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000878 DASWQN010000000
VERSION     DASWQN010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2458714"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..794)
                     /locus_tag="VGC82_14830"
     CDS             complement(105..794)
                     /locus_tag="VGC82_14830"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY0424590.1"
                     /translation="MNQIGLFEAIYSARAMRRLKPDPVPDELMTRVLDAAIRAPSGGN
                     AQNWIFIVVRDEAQRRRLGAVYRKASDEVAEIYAARGRPAHLTEAQYQRLMAGGSYLW
                     DHIGEAPVLLVPCLRKRDMPPREALPDAVAARYDAHLAHQDRIRGSSIYPAVQNIILA
                     CRGLGLGTLITTNHILYEDEVRAILGLPDDVYTFALMPVGFPLGKYGPLARRPVSEVA
                     FADRWGTNWPG"
     gene            complement(868..993)
                     /locus_tag="VGC82_14835"
     CDS             complement(868..993)
                     /locus_tag="VGC82_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424591.1"
                     /translation="MAAGAQQNSLKLVELEDIPLSYLPGDARRVRVRMVGDIGSG"
     gene            1570..2877
                     /locus_tag="VGC82_14840"
     CDS             1570..2877
                     /locus_tag="VGC82_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0424592.1"
                     /translation="MASGNTTPRSKGSSRRGLLKGAAAAIGTAVGSDAIRGFPTIWAQ
                     NIKDIVLHHAGPPVTAIPAIAEKASKDLGFTVQMQASENADLLNRFLSQSSAIDCADI
                     SLVYMRYLIGRNVLQAIPQSKFAHWDKTIPLFTKGEYPDGRKVPQQGITPTMILYATD
                     ATGQKATGGAPTEWLTGIPTVTNGDTLGIRPDLVGRKVDSWADVLSQEFKGKSAIQDN
                     PTVGVIDVAMALEARGDIKYGNKGNMTKEEIDKTVKAMLELKQSGQFRSFWSSFDQSV
                     NLMASGEVVIQSMWSPAVAAVRSRGIPCTFQPLKEGYRGWGYTMSVMKHVSGIKLDAF
                     YDYMNWYTSGFEGAFIARQGYYAAQPENTRKYLTENEYGYWFEGKAAETDILSPFGKV
                     MEKAGNVRDGGALWDRLGNIAVWNSVMDEDRYLTRRWNEFITS"
     gene            3064..4146
                     /gene="rutA"
                     /locus_tag="VGC82_14845"
     CDS             3064..4146
                     /gene="rutA"
                     /locus_tag="VGC82_14845"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HEY0424593.1"
                     /translation="MQLGVFIPIGNNGWLISTTSPQYKPSFDLNKTIVDKAEQFGFDF
                     ALSMIKLHGFGGPSGFWDYNLESFTLMAGLAATTTKIKLFATCAVLTMPPPIAARMAV
                     TIDSISHGRFGVNIISGWQRREYTQLGMWPGAEHYKRRYDYCAEYVTIMKELWETGRS
                     DFKGDFFQMDDCRCLPMPTAKIPIICAAQSDAGTRYAAEHADYNFCASFGYNAPTAVA
                     PSVVRLVKAKEQTGRDCGALILTMIIADETDAAANAKWEHYKAGTDLEALAYRDEQAQ
                     DDPSTDIYAQPNRRRILGTNHLPTNQGVLVGSYASVARMLDELAEVPGVRGVMMTFDD
                     FVIGMEQFGTRIMPLMRSRNEISMAA"
     gene            4143..>4378
                     /locus_tag="VGC82_14850"
     CDS             4143..>4378
                     /locus_tag="VGC82_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424594.1"
                     /translation="MNTAGLSSILPFGLRGRTAVAAGRRGSTRLPPWLVSWMQVGPLA
                     LILVVLFALPTTLFLIVSFWDYDRTGIYPAFLLD"
BASE COUNT          889 a         1347 c         1326 g          816 t
ORIGIN      
        1 gatgatgctt ggagcaggaa ccccctgacc ccggtcaatc acaccatcac tctgagggac
       61 gaccacttta ctcgccaact ccggcagccg gcccgttacc ccatctagcc aggccagttc
      121 gtgccccagc ggtcggcgaa ggcaacctct gaaacagggc gacgcgccaa cggaccgtac
      181 ttcccaagcg gaaaaccgac cggcatcagt gcaaacgtat agacatcatc cggcaggcca
      241 agtatcgctc ggacttcgtc ctcatacagg atgtggttgg tggtaatcag ggttccgaga
      301 ccaaggcccc ggcacgccag gatgatgttc tgcacggccg gatagatgct cgaaccgcgg
      361 atccgatcct gatgagccag gtgggcatca taacgcgccg cgacagcgtc cggcagcgcc
      421 tcccgcggcg gcatgtcgcg cttccgcagg cagggaacga gtaggaccgg cgcctcgccg
      481 atgtgatccc acagatagct gccgccggcc atcagccttt gatactgcgc ctccgtcaga
      541 tgcgccgggc gtccacgcgc ggcgtagatt tcggcgacct cgtccgaggc cttgcggtaa
      601 acggcgccca ggcggcggcg ctgcgcctcg tcgcgcacaa cgataaagat ccagttctgc
      661 gcattgccgc cggacggcgc ccggatggcc gcgtccagga cccgggtcat cagttcatcc
      721 ggcaccggat caggcttaag ccggcgcatc gcgcgagcgc tgtagatggc ctcgaacagt
      781 ccgatctgat tcatgcctgg tgcctttgcg agagctacat gatggcgtgt gcggcgcgtc
      841 tgttcacctc ggccggaagc gccgttgtca gccggaaccg atgtcgccca ccatgcgaac
      901 acgaacacgg cgggcatctc ctggcaggta ggatagcggg atatcctcca attcgaccag
      961 cttcaggctg ttctgctggg caccggctgc caccgcgcgc tttttgcctc agtctgggcc
     1021 gccagtatcg cctgatcgcg agtcaggttg ggccgtgctg gttggcatcg tcggccgtca
     1081 gctctggctg gccgcccgtg gtgcggacgg ccaaacggcg atggaggtgt ttgccggtga
     1141 cgtgatcgcc ggggacgcgc cgttgtcgtt cttcagttcc gtcggcacca gtcttcgtcg
     1201 gcaccagtct tcgtcggaat ctctgacaac cgatcgacgc tgccaccggg ccggtggcgt
     1261 taccgataag cttggtgtgc cgaatcgcca cctaccgtta agaaccctgt cagcagatat
     1321 ttcgcattcg gacgcccagc cgaggcagct tgttgagttg ggtagagacc gaccgcggcc
     1381 atgcatctat gaatgattat tccataggca tttctggcac aaaatggtgc ataagcacac
     1441 atactatcct ataccgcgct gaataaaaat ttaatgatcg aaaaacagcc atgttgcatt
     1501 gcagcgtgag cgggatcggg ttagcgtctg cttcgatcct gcccatatca tacccgagga
     1561 tagacacgca tggcatcagg caacaccacg cccaggtcga agggcagctc ccgccgaggc
     1621 cttttgaaag gtgccgcggc ggccatcggc actgccgtcg gttcggatgc gatacgcggc
     1681 ttcccgacca tctgggcgca aaacatcaag gacatcgtcc tgcatcacgc cggccctccg
     1741 gtcactgcca ttccggcgat cgccgagaag gccagcaagg accttggctt caccgtgcag
     1801 atgcaggcct cggaaaacgc cgatctgttg aaccgcttcc tgtcgcagtc cagcgccatc
     1861 gactgtgccg acatcagcct ggtgtatatg cgctacctga tcggccggaa cgtgctgcag
     1921 gcgatcccgc agtcgaaatt cgcgcactgg gacaagacca tcccgctgtt caccaagggc
     1981 gaatacccgg acggccgcaa ggtgccgcag cagggcatca cgccgacgat gatcctgtat
     2041 gcgaccgacg ctaccggcca aaaagccacc ggcggcgcgc cgaccgagtg gctcaccggc
     2101 attcccacgg taaccaacgg cgatacgctt ggcatccgcc ccgacctggt tggccgcaag
     2161 gtcgatagct gggcggacgt gctcagccag gagttcaagg gcaagtcggc gatccaggac
     2221 aacccgaccg ttggcgtcat tgacgtagca atggccctgg aagcgcgcgg cgatatcaaa
     2281 tacggcaaca aaggcaacat gacgaaggag gagatcgaca agaccgtcaa ggcgatgctg
     2341 gagcttaaac agtccggcca gttccgctcg ttctggtcgt cgttcgatca gtcggtgaac
     2401 ctgatggcct ccggcgaggt ggtgatccag tcgatgtggt cgccggccgt ggccgccgtg
     2461 cgttcccgcg gcatcccctg caccttccag ccgctgaagg aaggctatcg cggctggggc
     2521 tataccatga gcgtgatgaa gcatgtgtcg ggcatcaagc tggatgcgtt ctatgactac
     2581 atgaactggt acacgtcagg tttcgaaggc gcgtttatcg cccgtcaggg ctactacgcc
     2641 gctcagcccg agaacacgcg gaaatacctg accgagaacg agtatggcta ctggttcgag
     2701 ggcaaggcgg ccgaaaccga catcctcagc ccgttcggca aggtgatgga aaaagccggc
     2761 aacgtgcgcg acggcggtgc cttgtgggat cgcctcggca atatcgcggt gtggaactcg
     2821 gtgatggacg aagaccgtta cctgacccgc cgctggaacg aattcatcac gtcctgagca
     2881 tgatcacgca aagcggaacg ttcggagcgt gtgaatcatg cgattaaacg aagagcatgg
     2941 tcacgcaaag cggaacgttc gggccggtga atcatgcaac caacaaagtc aatcatgcga
     3001 tcaacaaagt cacagagcat tttcagcttg tacagttgaa atgctccgag ccggagatac
     3061 ttgatgcagc ttggtgtttt cattcccatc ggcaacaacg gttggctgat ctccacgacg
     3121 tcgccgcaat acaagccgag tttcgacctg aacaagacca ttgtcgataa ggccgaacag
     3181 tttggctttg acttcgcgct gtcgatgatc aagctgcacg gctttggcgg gccgtccggt
     3241 ttctgggact acaacctgga atccttcacc ttgatggctg ggcttgccgc gacgacgacc
     3301 aagataaagc tgttcgcgac gtgcgcggtg ctgaccatgc cgccacccat cgcggcgcgg
     3361 atggcggtga ccatcgacag catctcgcac ggacgcttcg gggtgaacat catctccggc
     3421 tggcagcgcc gcgaatacac ccagctgggc atgtggccgg gtgccgaaca ctacaagcgc
     3481 cgctacgact actgcgcgga atacgtcacc atcatgaagg aattgtggga gaccgggcgc
     3541 tccgacttca agggtgattt cttccagatg gacgattgcc gctgtctgcc gatgccgacc
     3601 gcgaagatcc ccatcatctg tgccgcgcag agcgacgccg gaacacgcta cgccgcggaa
     3661 cacgcggatt ataatttctg cgccagcttc ggctacaacg ccccgaccgc cgtcgccccc
     3721 agcgtggttc ggctggtcaa ggccaaagag caaaccggcc gcgactgcgg ggcgctgatc
     3781 ctgaccatga tcatcgccga cgaaaccgat gcggcggcaa atgcgaagtg ggaacattac
     3841 aaggccggca ccgacctcga agcattagcc tatcgcgatg agcaggctca ggacgatccc
     3901 agtacggata tctatgcgca gccgaaccgg cgccgcatcc tggggacgaa ccacttgccg
     3961 accaaccagg gcgtgctggt cggatcctat gcctcggttg cccggatgct cgacgaactc
     4021 gcggaagtgc ccggcgtgcg cggcgtgatg atgacgttcg acgatttcgt catcggcatg
     4081 gagcagttcg gcacgcgcat catgccgctc atgcggtctc gcaatgaaat ttcgatggcc
     4141 gcatgaatac ggctggcctg agttcgatcc tgccgttcgg cttgcgtggc cggacggcag
     4201 tggcggcggg caggcgtggg tcaacccgtt tgccgccctg gctggtatcg tggatgcagg
     4261 ttgggccgct ggcactgatc ctggtggtgt tgttcgcgct accgaccacg ctgttcttga
     4321 tcgtcagctt ctgggactac gaccgcaccg gcatttatcc ggccttcctg ctggacaa
//
LOCUS       DASWQN010000879         5801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_479675, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000879 DASWQN010000000
VERSION     DASWQN010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479675"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VGC82_14855"
     CDS             <1..705
                     /locus_tag="VGC82_14855"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424595.1"
                     /translation="SMPQSPGAEWRKKWSSYDPALANKMLDGIGLAKKDNEGFRLRTD
                     RGERLRIQVIAIAAFLPYPKLSEMVADQWRKIGIQADVREMERGLGFAKQTNNEHQLF
                     VWNNGGTELLYLFPTWAVPVIPGAAFAPDYARWYSSGGTQGAKPDDPNILKIFDLYAE
                     AAGLKAEERNRNAQQIWKILVDQQYGIGTVGQSPAGLGVRLVSNRLGNIAARVCNAQH
                     CRTPGNSHPETWYFKT"
     gene            710..1702
                     /locus_tag="VGC82_14860"
     CDS             710..1702
                     /locus_tag="VGC82_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0424596.1"
                     /translation="MLTYLLRRLGLAIITVFAITLVTFVILHLPPVDFVTAYAAQAAA
                     SGSAISAAEADAMRLAYGLNEPLWVQYWKWLSMVAVGDFGRSFEYGRPVTEVIGDRLW
                     LTILLSIGAIIVTWSLALPIGIYSAVRQYSAGDYIFTFLAFIGLAIPSFLLALIVMYA
                     GLRLFNTNVGGLFSSDYQLAPWSWGKVWDLIKHLPVPMLILALPGIAQLVRIMRANLL
                     DELRRPYVVTARARGLAERRVIMRYPVRAALNPFASTIGYLLPYTVSGSVIISVVLSL
                     PTVGPLLLHSLISQDMLLGSSIILLLGVLTVFGTLLSDLLLMLIDPRIRLGQLR"
     gene            1699..2832
                     /locus_tag="VGC82_14865"
     CDS             1699..2832
                     /locus_tag="VGC82_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0424597.1"
                     /translation="MSTTTIAEPLVTSEAEVRVAVATQRQLIWWRFRKHKLAVASAGV
                     VLAFYLIAVFADFIAYSGPFDTLAARSYIPPQPIHWLDADGHFHPQVYALKGKRDPRT
                     FKLSYAPDPSEPRDLVLFGRGYEYNLFGLFPTDVHLLGVSGGRPQDSLFLLGTDQLGR
                     DVFSRLVLATQTSLTIGLVGVSLSLVLGVLLGGLSGLYGGWVDVVIQRVIEILRSIPT
                     IPLWMGLAAAVPTSWSPQRVYFAITLIISLIGWTELARVVRGRFLALREEDFITAAEL
                     AGASRLRIIFKHMVPSFLSHIIAAVSLALPAMIISETTLSFLGLGLRPPAISWGVLLQ
                     DAQNLQTLGLAPWLLVVAVPVIVVILAFNFLGDGLRDAADPYG"
     gene            2825..3835
                     /locus_tag="VGC82_14870"
     CDS             2825..3835
                     /locus_tag="VGC82_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0424598.1"
                     /translation="MAEPILQVRDLHTHFFADEGVVRAVDGTSFELYAGRTLGIVGES
                     GCGKSVTARSLLRIVERPGRIVSGEILLRRADGTVTDLVKLPADGTEMRAIRGGEIGL
                     VFQEPMSSLSAFHTIGDQLIEAIRLHGGLSKRAARSRAIELLALVGIPRPSQRVDAYS
                     FELSGGLRQRVMVALALSAEPRVLIADEPTTALDVTTQAQILDLIRRLQQERDLAVIL
                     ITHDMGVIAEMADDVVVMYLGHEVEKGPVDAIFHAPRHPYTQSLLRSIPSVLAEPRAR
                     LATISGSIPHPFARPPGCPFHPRCPDYMRGVCEQSFPAEILIGERHAAACHLYTSASR
                     SA"
     gene            3832..4845
                     /locus_tag="VGC82_14875"
     CDS             3832..4845
                     /locus_tag="VGC82_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY0424599.1"
                     /translation="MTQVLDVRSLRKHYPIRQGLLGRVTARIRAVDDVSFSIARGETL
                     SLVGESGCGKTTTSRCILRAIAPTSGEIRFRGASGEEIDVATLNRKQLRPLRRQMQMI
                     FQDPFSSLNPRMTIAEIIGEPLLVNGLTNTTERNRRVAELLELVQLPNAYLNRFPHAF
                     SGGQRQRIGIARALALNPSLIVADEPVSALDVSVQAQIVNLMLDLQDKLGMAYLFVAH
                     DLSIVKHVSHRVAVMYVGRIVEVAPTTSLFTTPRHPYTEALLSAVPVPDPRLRAQRIV
                     LEGDVADPSDPPPGCHFHPRCRYATDRCRVETPELTEIAPGHAVRCLRARELSLAGVV
                     APA"
     gene            5049..>5801
                     /locus_tag="VGC82_14880"
     CDS             5049..>5801
                     /locus_tag="VGC82_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424600.1"
                     /translation="MKPPRLLYCVALIVLATAGITPARAAPFMIVGNDEKPGTDAQGK
                     PIVNAAGNDTVLIVDVAEPEAPKIVATLQLENSIVGPPTNLAISPNGDIALVADSMTV
                     IEENGTRKLVPTDKLFVIGLKATPPQLIQTLTLGKQPSGLSFNSNGDLALVCNRADGS
                     ISVLKIEGKQVTPIGTVAIGPGVSHAVFTPDGKHALALKSPDNRIAMLDIDRDKVTYN
                     KLDLPTYPFPYNIVVTPDSRLAITADNGNNGSS"
BASE COUNT          965 a         1790 c         1858 g         1188 t
ORIGIN      
        1 agcatgccgc aaagtcccgg tgcggaatgg cggaagaagt ggtccagcta tgatccggca
       61 ctcgcgaaca agatgctgga cggcatcggg ctggcgaaga aggacaatga gggtttccgc
      121 ctgcgcaccg accgcggcga gcgactgcgc atccaggtga tcgcgatcgc ggccttcctg
      181 ccatacccca agctgtccga gatggttgcg gaccagtggc gcaagatcgg catccaggca
      241 gatgtccgcg agatggagcg aggacttggc ttcgccaaac aaaccaacaa tgaacaccag
      301 ttgtttgttt ggaacaatgg tggaaccgag ctgctgtatt tgtttccgac ctgggcggtg
      361 ccggtcattc caggtgctgc gttcgcaccc gactatgcgc ggtggtattc gtcgggcggc
      421 acgcagggag ccaagcccga tgatccgaat atcctgaaga tcttcgacct gtatgccgag
      481 gcggcgggtc tgaaggcgga ggaacgcaac cgcaacgcgc agcagatctg gaagattttg
      541 gtcgatcaac agtatgggat cggcaccgtc ggacaatccc cggcgggtct cggtgtgcgc
      601 ctggtgtcga accggctggg caacatcgcc gctcgggttt gcaatgcgca gcattgccgc
      661 acgccgggaa attcccatcc ggaaacctgg tacttcaaga cctgatgccg tgctgaccta
      721 tttgttgcga cggctgggac tggcgattat cacggtgttc gccatcacgc tggtgacctt
      781 cgttatcctg cacctgccgc cggtcgattt cgttactgcc tatgccgcgc aggcggctgc
      841 atcgggttcc gcgatctcgg cggccgaggc ggatgccatg cggctggcct acgggctgaa
      901 cgaaccgctc tgggtgcagt attggaaatg gctgtcgatg gtcgcggtcg gggatttcgg
      961 ccgctccttc gaatacgggc ggccggtgac cgaggtgatc ggcgaccggc tgtggttgac
     1021 gattctgctg tcgatcggcg ccatcatcgt cacctggagt ttggcgctgc cgatcgggat
     1081 ctactccgcg gtgcggcaat attccgccgg tgactacatc tttacgttcc tggccttcat
     1141 cgggctggcg atcccgagtt tcctgctcgc gctgatcgtg atgtatgccg ggcttcgact
     1201 gttcaacacc aatgtcggcg gcctgttttc ctcggattac cagttggcgc catggtcctg
     1261 gggaaaggtc tgggatctga tcaagcacct tccggtgccg atgctgatcc ttgcgctgcc
     1321 gggcatcgcg caacttgtgc ggatcatgcg cgccaatctg ctggatgagt tgcgtcggcc
     1381 ctacgtggtg acggcgcggg cgcgcggctt ggcggaacgg cgggtgatca tgagatatcc
     1441 ggtgcgtgcg gcgctgaacc cgttcgcctc gacgatcggc tatctgctgc cctataccgt
     1501 gtcgggcagc gtgatcatct cggtggttct tagcctgccg accgtgggac cgctgttgct
     1561 gcattcgctg atttcccagg atatgctgct gggatcgtcg atcatcctgc tgctgggcgt
     1621 cctgacggtg ttcggcacct tgctgtcgga cctgttgctg atgttgatcg atccacgcat
     1681 ccggctgggg cagttgcgtt gagcaccacc accattgcag aaccgctcgt caccagcgaa
     1741 gccgaagtcc gcgtcgcggt cgccacccag cgtcagttga tctggtggcg gtttcgcaag
     1801 cacaagctgg ccgtggccag cgccggggtc gtgctggcgt tctacctgat cgcggtgttc
     1861 gccgatttca tcgcttactc cgggccattc gatacattgg cggcccgcag ctatatcccg
     1921 ccgcagccga tccactggct ggatgccgac ggacatttcc atccgcaggt ctatgcgctc
     1981 aagggcaagc gcgatccccg cacgttcaaa ctgtcctacg cgccggaccc aagcgagcct
     2041 cgggatctcg tgttgttcgg gcggggctac gagtataatt tgtttgggct gttcccgacc
     2101 gacgtacatt tgctgggcgt cagcggcggc cggccgcagg attcactgtt tcttctcggc
     2161 accgatcaac tgggccgcga tgtgttctcg cgcctggtgc tggcgaccca gacgtccctg
     2221 acgatcgggc ttgtcggcgt ttcccttagt ttggtactgg gtgttctgct gggcggcctg
     2281 tccgggctgt atggtggttg ggtcgatgtg gtgatccagc gggtgatcga aatcctgcgg
     2341 tcgattccga ccattccgct atggatgggc ctggccgcgg cggtgccgac aagctggtcg
     2401 ccgcagcgcg tgtatttcgc catcaccctg atcatttcgt tgatcggatg gaccgaactg
     2461 gcgcgcgtgg tgcgcgggcg gttcctggca ttgcgggagg aagatttcat caccgccgcc
     2521 gaactcgcag gcgcgtctcg gctgcgcatc atcttcaagc acatggtgcc gtcgttcctg
     2581 tcgcacatca tcgccgcggt cagtttggcg ctgccggcga tgatcatcag cgagacgacg
     2641 ttgtcgttcc tcgggctagg gttgcggcca ccggcgattt cctggggcgt gctccttcag
     2701 gatgcgcaga atctgcagac gcttggactg gcaccgtggc tgctggtcgt tgcggtgccg
     2761 gtgatcgtgg tgatcctggc gttcaacttc ctcggcgacg ggctgcgcga cgcagccgac
     2821 ccctatggct gagccgattc tgcaagtgcg cgatctgcac acgcatttct tcgccgacga
     2881 gggcgtggtg cgcgcggtcg atggtacctc gttcgagttg tatgcgggcc gcacgctggg
     2941 gatcgtcggc gaatccggct gcggcaagag cgtgacggcg cgctcgctgc tgcggatcgt
     3001 cgagcggcca gggcgcatcg tcagtggcga aattctgctg cgccgcgcgg atggtacggt
     3061 gaccgatctg gtcaagctac cggcggatgg caccgaaatg cgcgccattc gcggcgggga
     3121 gattggcctg gtgttccagg agcccatgag ttcgctgtcg gcgttccata cgatcggcga
     3181 ccagttgatc gaggcgatac gtttgcacgg cggcctgtcg aaacgcgccg ctcggtcgcg
     3241 agcgatcgaa ctgctggcgt tggtcggcat tccgcgtccg tcacagcggg tcgatgccta
     3301 ttcgtttgaa ctgtccggcg gactacgcca gcgggtcatg gtcgcgctgg cgctgtccgc
     3361 ggaaccacgc gtgctgatag ccgacgaacc gacgacggcg ctggacgtga cgacgcaggc
     3421 gcagatcctc gatctgatcc gccgcctgca gcaggaacgc gatctcgcgg tgatcctgat
     3481 cacccacgac atgggggtaa tcgcggagat ggccgacgat gtcgtggtga tgtatctcgg
     3541 ccacgaagtc gaaaaaggcc cggtggacgc catcttccat gcgccgcggc atccgtacac
     3601 ccagtcgctg ctgcgctcga tcccgagcgt actggcggaa cctcgagcac ggttggcgac
     3661 gatcagcggg tcgatcccgc acccgttcgc ccgcccgccg ggctgcccat tccatccgcg
     3721 atgcccggat tacatgcgcg gcgtctgcga gcagagtttc cccgccgaga tcctgatcgg
     3781 ggaacgccac gcggcagcct gccaccttta tacatctgca tcgcggtcag cgtgacacag
     3841 gtcctcgatg tccggtcgtt gcgcaagcat tatcccattc gtcagggact gctcggacgc
     3901 gtcaccgcac ggattcgcgc ggtggacgat gtcagcttca gcattgcccg tggcgagacg
     3961 ttgtcgctgg tcggtgaaag cggctgcggc aagaccacga cctcgcgttg tatccttcgg
     4021 gctatcgcgc ccaccagcgg cgagatccga tttcgcggcg catcaggcga ggagatcgac
     4081 gttgcgaccc tgaaccgcaa gcagttgcgg cccctgcggc ggcaaatgca gatgatcttc
     4141 caggacccgt tttcctcgct caatccgcgc atgaccatcg cggaaatcat cggcgagccg
     4201 ctgctggtca atggcctgac caacaccacc gagcgtaacc ggcgcgtggc cgaattgctg
     4261 gaactggtgc agttgcccaa cgcctacctg aaccgctttc ctcatgcttt ctccggcggc
     4321 cagcggcagc gcatcggcat tgcccgcgcc ttggcgctca acccatcgct gatcgtggcg
     4381 gacgaaccgg tgtcggcgct ggatgtctcg gtgcaggcgc agatcgtcaa tctgatgctg
     4441 gatcttcagg acaagcttgg gatggcctac ttgttcgtgg cgcacgatct atcgatcgtg
     4501 aaacatgtca gccatcgcgt cgcggtgatg tatgtcgggc gcattgtcga ggtcgcgcca
     4561 accacctcgc tgtttaccac gccacggcat ccctacaccg aggctttgct gtccgccgtt
     4621 cccgtgcccg atccgcgtct gcgcgcgcaa cgcatcgtgc ttgagggcga tgtcgcggat
     4681 ccgtcggacc cgccgccggg ctgccatttc catcctcgct gccgctatgc cacggaccgg
     4741 tgccgcgtgg aaacgccgga actgaccgag atcgcaccgg gtcatgcggt acgttgcctg
     4801 cgtgctcgtg aactgtcctt ggccggggtg gtggcgccgg cttgaagggc tttggccgcg
     4861 cgtcgtcgcg cttatcggcg gcgcggcgac cgtcctggcg tcgaagttcg agcatgccgc
     4921 agggggcgct tgccactgtt cgacatggct ctcagactgg tcccaccccg gcgtttggtt
     4981 gccaggcgcc gggtgaatcg acccaccaaa tcagcgtcca gcagaatcaa aaacaccggg
     5041 aggaaaacat gaagccgccg cggctcctgt attgcgtcgc tcttatcgtc ctggctactg
     5101 ccggcatcac tccggcacga gccgcgccct tcatgatcgt cggcaacgat gaaaagcctg
     5161 gaactgacgc gcagggaaag cccatcgtga acgccgccgg caatgacacc gtgctgatcg
     5221 ttgacgtggc agagcccgag gcgccgaaga tcgtcgcgac actgcagctc gagaattcca
     5281 ttgtcggccc gccgaccaat ctggcgattt cgccgaacgg cgatatcgcg ttggtcgcgg
     5341 attccatgac ggtcatcgag gagaacggca cgcgcaaact ggtgccgacc gacaagctgt
     5401 tcgttatcgg cctgaaggcc acgccgccgc agctcataca aacactgacg ctcggcaagc
     5461 agccgtcggg gctttcgttc aattccaacg gcgacctggc gctggtgtgc aatcgagccg
     5521 acggctcgat cagcgtgttg aagatcgaag gcaagcaggt gacgccgatc ggcaccgtcg
     5581 cgatcggccc gggcgtgtcc catgccgttt tcaccccgga cggcaaacac gctttggccc
     5641 tgaagtcacc ggacaaccgg attgcgatgc tggatatcga tcgcgacaaa gtcacctaca
     5701 acaagttgga tttgccgacc tacccgttcc cttacaacat cgtggtcaca cccgattcga
     5761 ggctggcgat caccgccgac aacggcaaca atggttcctc c
//
LOCUS       DASWQN010000880         5207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_216443, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000880 DASWQN010000000
VERSION     DASWQN010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216443"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..490
                     /locus_tag="VGC82_14885"
     CDS             260..490
                     /locus_tag="VGC82_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424601.1"
                     /translation="MTTVTHDMLNDCQALRRSPTVRSDGLQGRISRIVGLWRERRRQR
                     QSFDTFGHRELRELGLSRWEVERELAKPFWRG"
     gene            complement(496..1974)
                     /gene="glpK"
                     /locus_tag="VGC82_14890"
     CDS             complement(496..1974)
                     /gene="glpK"
                     /locus_tag="VGC82_14890"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY0424602.1"
                     /translation="MTRYIGAIDQGTTSSRFIVFDRGGNTIAVAQREHRQIYPNPGWV
                     EHDPLEIRDNTMAVIEEALRSADLEATDLAAVGVTNQRETTLLWDRSTGLPVYNALVW
                     QDTRVDPMVAEMARDGGQDRFRATTGLPLASYFSALKLRWLLDTVPGARQRAEAGDLA
                     FGTVDSWILWHLTGQHITDVTNASRTQLMDLATLEWDAGLLAAFGVPPAVLPRIVPSS
                     CVYGLARAPLAGVPVAGILGDQQAALMGQACFIPGEAKNTYGTGCFLLMHTGTTPTPS
                     KHGLITTVAAKLGDRPATYALEGSIAITGALVQWLRDNLGLIKSSAEIETLALSVPDN
                     GDVYFVPAFSGLYAPWWRADARGTIAGLTRFASAGHLARAALEATAYQTMDVVRAMEQ
                     DSGIAMASLRVDGGMVVNRTLMQFQADMLDVPVVRPSCLETTALGAAYAAGLAVGYWT
                     GLDDLRVNWAEAERWTPEMDRDRRASLTASWHKAVDRSMAWG"
     gene            2098..2826
                     /locus_tag="VGC82_14895"
     CDS             2098..2826
                     /locus_tag="VGC82_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0424603.1"
                     /translation="MSQRFAGRTAVVTGGVSGIGAGIARRLAAEGATLSIWDMDEAAL
                     ARSGISHSVALDVTDPDAVRRAAEATAAALGKIDIMVTSAGVTGPNHPTWEYPIAAWD
                     RVIDINLKGVFYCCHAVVPFMRTNGYGRIVNIASIAGKEGNPNASAYSASKAGVIGLT
                     KSLGKELAATQIRVNCVNPAAVKTPLFEQMTPEQVNWMLSKIPIGRFGAIDEVASLVL
                     WLASEEASFSTGAVFDISGGRATY"
     gene            2888..4297
                     /locus_tag="VGC82_14900"
     CDS             2888..4297
                     /locus_tag="VGC82_14900"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0424604.1"
                     /translation="MNDEDAGFLPATQLAELIRTKQISPVEYMRALLRRIEALEPKVN
                     AFVHLAADQAMDAARAAEAKLMSGEPIGRLHGLPVTIKDLAITKDMPTQQGSLIYQGN
                     QPAEDAPIVSRLKHEGAIILGKTTTSEFGWTGVSRSPLTGITHNPWRFGFNAGASSAG
                     AGAAAAAGFGPLHQGSDGAGSIRMPAHFCGVFGLKPSFGRVPSYPVNTGDLTSHVGPL
                     TRSVADSAMFLEVAAGPHPLDYTSLEAGPAAYQARLHEGIKGKRIAYSPDLGHARVDP
                     GVAALVKKAAERFSEAGAVVEEMATPWAAAGPDLIRFFWSAHQTRFKPFLEQWEQLMD
                     PGLVACIKASSNVSIAEYQAARERKMAYIADIHRWFEDWDFLLTPTVSVAAFPAEKLM
                     PDHWPRHEWDWVSWAEFSYPFNMAWNPAASVPCGFTADGLPVGLQIVGKRFDDLGVLQ
                     ASAAFESIQPWADKRPALA"
     gene            4337..4996
                     /locus_tag="VGC82_14905"
     CDS             4337..4996
                     /locus_tag="VGC82_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY0424605.1"
                     /translation="MAEALHAFLLGFPALFSIVNPISGAFIFRGVTASRGPDVQAKLA
                     RLVALNSFGVMMGALWAGSYVLAFFGISLAALRVAGGLVVALSGWSLLNAPEQREVSK
                     QEQAASSEGLEDVALFPLTIPFTTGPGTISVAVALGAGHPRLFAGLGWFFLGMTGAAI
                     AMTATIWVTYLYADRLTRMMGPTGTRTITRISAFLLLCIGVQILITGVEDVLGPLLAA
                     R"
BASE COUNT          898 a         1725 c         1648 g          936 t
ORIGIN      
        1 ccattaaggg aagggagtag catggcgcca acacgttaga gcattttcag cccgcctaca
       61 ggtcgaagat gctctacagg ccgaaggcgc tctgcctatt ttgcttgatc gcatgattca
      121 cacgcctggg acgttccgcc cggcgtgatc atgctctcgc atgaattttt gtcatgaaca
      181 ggctgacaaa ccatcgtttg tcctgggccg gcgaagcgcc caggtttccg ccagatttaa
      241 caggataccg gaggctacga tgaccactgt cacccatgat atgctgaacg attgtcaggc
      301 gcttcgccgc tccccaacgg tccgctccga cgggttgcaa ggacggatca gccgcattgt
      361 tggcttatgg cgggagcggc gccggcagcg gcagtcgttc gatactttcg gccaccgcga
      421 actgcgtgaa ctgggtcttt ctcggtggga ggtggagcgc gagttggcaa agccgttctg
      481 gcggggctga ctgtctcatc cccaggccat cgagcgatcg accgccttgt gccacgaggc
      541 ggtcagcgag gcgcgccgat cccgatccat ctctggcgtc caccgctccg cctccgccca
      601 attgacccgt agatcgtcga gcccagtcca ataaccgacc gctagtccgg cggcataggc
      661 agcccccagt gccgtcgtct ccagacagga cgggcgtacc acaggcacat ccagcatgtc
      721 cgcctgaaac tgcatcagcg tgcggttgac caccattccg ccatccaccc gcagggacgc
      781 catcgcaatt ccgctgtcct gctccatcgc ccggacaacg tccatggtct ggtaagcggt
      841 cgcctccagc gcggccctcg ccagatgacc ggccgaggca aaccgcgtca gcccggcgat
      901 cgtcccgcgc gcatccgccc gccaccaggg cgcgtacaga ccggagaatg cgggcacgaa
      961 atacacgtcg ccattatccg gcacggatag tgcaagagtc tcgatctcgg cgctgctctt
     1021 gatcaaaccg aggttgtcgc gcagccactg caccagggca ccggtgatcg cgatcgagcc
     1081 ctccagcgca taggtcgccg gccggtcgcc cagcttcgcc gccacggtgg tgatcaaccc
     1141 gtgcttcgac ggcgttgggg tggttcccgt gtgcatcagc aggaagcatc cggtgccgta
     1201 ggtgttcttc gcctcgcccg gaatgaaaca tgcctggccc atcaacgcgg cctgctgatc
     1261 gccgaggatg ccggcgaccg gcacccccgc cagcggcgcc cgcgccagcc cgtacacgca
     1321 ggacgacggc acgatccgcg gcagcaccgc cggcggcaca ccgaacgccg ccagcagtcc
     1381 ggcgtcccat tccagcgttg ccaaatccat cagttgcgta cggctggcgt tcgtcacgtc
     1441 ggtgatgtgc tgcccggtca ggtgccacag gatccaactg tccacggtgc cgaatgcgag
     1501 gtctcctgct tcggcccttt ggcgggcacc gggcaccgtg tccagcagcc accgcagctt
     1561 cagcgccgaa aagtagctgg cgagcggcag gccggtcgtg gcgcgaaacc ggtcctggcc
     1621 accgtcgcgc gccatctccg caaccatcgg atcgacccgc gtgtcctgcc agaccaaggc
     1681 gttgtaaacc ggcagcccgg tcgagcgatc ccacagcaac gtcgtttcgc gctgattggt
     1741 aaccccgacc gccgccagat cggttgcttc cagatcggcg gagcgcagcg cctcctcgat
     1801 cactgccatc gtgttgtccc ggatctcaag cggatcgtgt tcgacccatc cgggattggg
     1861 atagatctgc cggtgctccc gctgggccac ggcgatggtg tttccaccgc ggtcgaacac
     1921 gatgaagcgg ctggacgtgg tgccctggtc gatggcgccg atataccgtg tcattacatc
     1981 tccttcgggc gctcagtctg ccgcctcgcc ggtaaatccg acaacccatc ttgccgccgt
     2041 cgccgctccg gctttcactg tgacgagctc actttgcagg caacaaggag actgtccatg
     2101 tcgcaacgat tcgcagggcg caccgccgtc gtgaccggcg gcgtctccgg cattggtgcc
     2161 ggcattgccc ggcggctcgc cgctgaaggc gccacgctga gtatctggga catggacgaa
     2221 gccgctctcg cacggtccgg tatctcccat agcgtggcgc tcgatgtgac cgacccggac
     2281 gcagtgcgcc gcgccgccga ggcaactgcc gcggcgctgg ggaagatcga catcatggtg
     2341 acatcggccg gggtgaccgg gcccaaccac ccgacatggg agtatccgat tgccgcctgg
     2401 gaccgggtga tcgacatcaa cctgaagggc gtcttctatt gctgccacgc cgtcgtgccg
     2461 ttcatgcgga ccaatgggta tggccggatc gtcaatatcg catcgatcgc cggcaaggag
     2521 ggcaatccga acgcctctgc ctattcagcc tcgaaggctg gtgtcatcgg attgaccaag
     2581 tcgctcggca aggaactcgc ggcaacccag atccgcgtca actgcgtaaa cccggcggcg
     2641 gtgaagacgc ccctgttcga gcaaatgacc ccggagcagg tgaactggat gctgtcgaaa
     2701 atccccattg gccgcttcgg cgcgatcgat gaggtcgcct cgctggtcct gtggctggcc
     2761 agcgaggagg ccagcttctc caccggcgca gtgttcgaca tttccggcgg acgggcgaca
     2821 tactgagggt gggacacgtc ccgacagccg gcgcgacgac gttcgcttca agtagaaggg
     2881 gataacgatg aacgacgaag acgcgggttt cctgccggcc acccaactcg cggaactgat
     2941 ccgcaccaag cagatatccc cggtcgaata catgcgcgcc ctgctgcggc gaatcgaggc
     3001 gctggaaccg aaggtcaatg cgttcgtcca cctcgcggcc gaccaggcga tggacgccgc
     3061 acgcgcggcc gaagcgaaat tgatgtcggg cgagcctatc ggccgcctgc atggcttgcc
     3121 ggtcacgatc aaggacctgg cgatcaccaa ggacatgccg acgcagcagg gaagcctgat
     3181 ctaccaaggc aaccagccgg ccgaggacgc gccgatcgtg tcccgcctga agcatgaggg
     3241 cgccatcatc ctcggcaaga ccacgacatc ggagtttggc tggaccggcg tgtcccgctc
     3301 gcccttgacc ggcatcacgc acaacccgtg gcggttcggg ttcaatgccg gcgcctcctc
     3361 ggcaggggcg ggcgcggcgg cggcggctgg tttcggcccg ctgcaccagg gcagcgacgg
     3421 cgccggctcg atccggatgc cggcgcattt ctgcggcgtg ttcgggctga agccgagttt
     3481 cggccgagta ccgagctacc cggtcaacac cggggacctg acgtcgcatg tcggaccctt
     3541 gacgcgttcg gtcgcggaca gcgcgatgtt cctggaggtg gcggctggcc cgcacccgct
     3601 tgactacacc agcctggaag cgggtccggc cgcttatcaa gccaggctgc acgaggggat
     3661 caagggcaaa cgcatcgcgt acagccccga cctgggccac gcgcgcgtcg atccgggcgt
     3721 cgccgctctg gtgaaaaaag cagccgaacg attttccgaa gccggcgccg tcgtcgagga
     3781 aatggcgacg ccatgggcgg ctgccggccc ggacctgatc cggttcttct ggtcggccca
     3841 ccagacacga ttcaagccgt tcttggagca atgggagcag cttatggacc ccggcctggt
     3901 ggcctgcatc aaggcctcca gcaacgtctc gatcgccgag taccaggccg cgcgcgaacg
     3961 caagatggcc tacatcgccg acatccaccg ctggttcgag gactgggact tcctgctgac
     4021 gcccacggtc tcggtcgccg cctttccggc ggagaaattg atgcccgacc attggccgcg
     4081 acatgaatgg gactgggtga gctgggccga gttctcctat ccgttcaaca tggcctggaa
     4141 cccggcggcc agcgtgccct gcggcttcac cgcggacggg ttgccggtgg gcctgcagat
     4201 cgtcggcaag cgatttgacg atctcggcgt tctgcaagcc tcggcagcgt tcgagagcat
     4261 ccagccgtgg gcggataaac gtcccgcgct ggcctgacag aatcctggcc ccgcagcatc
     4321 ttggcctgac agcatcatgg cggaagccct gcacgccttc ctgctcggtt ttcccgcgct
     4381 gttctccatc gtcaacccga tcagcggcgc tttcattttc cgcggcgtca ccgccagccg
     4441 gggcccggac gttcaggcaa agctcgcgcg cctggtggcg ctgaattcct ttggggtgat
     4501 gatgggcgcg ctttgggccg ggtcctatgt gctggcgttc ttcggcatca gcctcgccgc
     4561 gttgcgggtt gccggcgggt tggtggtggc cttgagcggc tggagcctgc tgaacgcacc
     4621 cgaacaacgc gaggtgagca aacaggagca agccgcctca tccgaaggat tggaggatgt
     4681 cgcgttgttt ccgctgacga tcccgttcac caccggccct ggcacgatct cggtggcagt
     4741 ggccttgggt gccggtcatc cgcggctgtt cgccgggctg ggatggttct ttttgggcat
     4801 gaccggggcg gcgatcgcga tgaccgcgac gatctgggtg acttacctct atgcggacag
     4861 attgacccgg atgatgggcc ccaccggcac ccgcaccatt actcggatct cggctttcct
     4921 gctgttgtgc atcggcgtgc agatcctgat caccggtgtc gaggatgtgc tggggccgct
     4981 tctggcggcc cgatagaggc cgcaccttcc tgagtgtccg tccgcgtgaa tggccgcacg
     5041 acggtccttg gcggatgttg atttgaattg ctagactgct ccttacgagt cgcttgcttc
     5101 ctcgctttcc gcgggccaga agccaggctg acctcttgta ggaacctgac catgaagctg
     5161 aaggccgacc gcgccacctt gatcaaggcg ctggcccacg tccaaag
//
LOCUS       DASWQN010000881         2894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1272305, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000881 DASWQN010000000
VERSION     DASWQN010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272305"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VGC82_14910"
     CDS             complement(<1..409)
                     /locus_tag="VGC82_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEY0424606.1"
                     /translation="MKKHLLRQLAAGSILSSMLLVGSVQAQSTSEDLVKMEKNAANVV
                     MPTQTYDNQRYSTLSQIKANNVGKLQVAWTFSTGVLRGHEGAPLVVGDVMYVHAPFPN
                     TVFALDLNHDGKILWKYEPKQDPNVIPVMCCDTV"
     gene            783..1703
                     /locus_tag="VGC82_14915"
     CDS             783..1703
                     /locus_tag="VGC82_14915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HEY0424607.1"
                     /translation="MLCLVYLTTIFNPSQARSSAEYDKFPLDRVADGDYAHVGQIAVT
                     TRENEGDIANLGVIVGLDAVAVVDTGGSVRVGQELLAAVQSITSKPVRYVINTHEHPD
                     HIFGNAAFGPGVTFVGHHNLPAEMQKRGEFYLRSFRGVLGRQAIEQVRIIPPTLLVMD
                     EMTLDLGDRRLHLMAWNPAGHTDCDLTVLDETTGVLFAGDLVFMRHLPVLDGSLRGWL
                     SVLPRLAAVPARIVVPGHGPSAPWPQALDDERRYLTQLADDTRRLIAAGVPVTEAVLS
                     IGQSEREHWALFDDYNPRNATASFSELEWE"
     gene            complement(1681..2796)
                     /gene="pqqE"
                     /locus_tag="VGC82_14920"
     CDS             complement(1681..2796)
                     /gene="pqqE"
                     /locus_tag="VGC82_14920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEY0424608.1"
                     /translation="MTGLAPLGLLAELTHRCPLACPYCSNPLELENRADELDAAAWAR
                     VFQQAAAMGVLQIHLSGGEPASRRDLIEITQAASDAGLYTNLITSAIGLDADRLRQLK
                     AAGLDHVQVSIQDTDPAAADRIAGYDGAHARKQVFAQAVVRLGMALTVNAVIHRANIA
                     HLTELVDLAASLGAGRVEIAHAQYYGWALRNRSALMPTREQIDRAMQEMDQLQARYAG
                     KLSIDSVIPDYYARRPKPCMGGWGRRSLNVTPTGLVLPCHAAQTIPGLEFWSVRDHSL
                     ADIWANSPAFEAFRGTAWMKEPCRSCAFQQQDFGGCRCQALAITGDAAATDPSCDLSP
                     VHAKMQILAEQATAEPATTYVYRGRKVSKEATPIPVR"
BASE COUNT          558 a          879 c          856 g          601 t
ORIGIN      
        1 ttaccgtatc gcagcacatc accggaatga cgttcggatc ctgcttcggc tcgtatttcc
       61 acaagatctt tccgtcgtga ttgagatcga gtgcgaagac cgtgttggga aacggcgcat
      121 gaacgtacat cacatcaccc acaaccagcg gtgccccttc gtgcccgcgc aacacgccgg
      181 tcgagaaggt ccacgcgacc tgtagcttgc ccacgttgtt cgctttgatc tggctcaggg
      241 tcgaatagcg ctggttgtca taggtctgcg ttggcatcac cacgttcgcc gcgtttttct
      301 ccatcttaac gaggtcttcg ctggtgcttt gcgcttgcac gcttccaacg agcagcattg
      361 atgaaaggat gcttccggcc gcgagttgcc tcaacaggtg ttttttcata ttttcctcct
      421 aacggcgact ttatcagaca cggcgactta tccaggagcc catcggataa caccgatgtt
      481 ggtccagaaa tccaccgggc aaaaccaggt gactggttcg tttacctagc cagcgttatt
      541 atcgcgcttc cgcgcttttg tgcactgcag aaacttcggc tccgctactt gaacgccgtt
      601 taatccttag catgggccgt gcgcggtcaa gcttcatacc ggaagaaaca gatcaacatc
      661 acagagtgtt gagtcgatcg cgatttcggg cgctaccgaa tcccggtcct aacggacgga
      721 aaggaaacat ctttatgatg cactgcacca tgcggaccgg tccaaatttt cgttacttga
      781 caatgttatg cctggtttat ttgacaacca tcttcaatcc atcccaagcg cgatcttcgg
      841 cagaatacga caaatttccg ctcgatcggg ttgctgacgg cgattatgcg catgtgggcc
      901 agatagcggt gaccacgcgg gagaatgagg gcgacatcgc gaacctcggc gtcattgtcg
      961 gactcgacgc cgtcgcggtt gtggataccg gaggcagcgt ccgggtcgga caggaactgc
     1021 tcgccgcagt ccaatcgatc accagcaagc cggtccgata cgtcatcaac acgcacgaac
     1081 atcccgacca cattttcggc aacgcggcat tcggaccagg cgtgacgttt gtcgggcacc
     1141 ataatctgcc cgcggaaatg cagaagcgcg gtgagttcta tctgcgctcg tttcgcggcg
     1201 tcctggggcg gcaagcgatc gagcaggttc gcatcatccc gccaacgttg ctcgtgatgg
     1261 acgagatgac gctcgacctt ggcgacaggc gactgcatct tatggcgtgg aaccccgctg
     1321 ggcacaccga ctgcgacctg acggtattgg acgagaccac gggcgtgctg tttgccggcg
     1381 acctggtctt catgcgtcat ctgcctgtgc tggacggcag cctgcgcggc tggctgtcgg
     1441 tactgccacg tttggcagcg gtgccggcca gaattgtggt cccggggcat gggccatcgg
     1501 cgccatggcc gcaggcgctg gacgatgaac gccgatacct gacgcagctt gcggacgata
     1561 cgcgccgcct tatcgcagcg ggggtcccgg tcaccgaggc ggtgctttcg atcggtcagt
     1621 cggagcgcga acactgggcc ttgttcgacg actacaatcc gcgcaatgcc accgcttcgt
     1681 tcagcgaact ggaatgggag tagcttcttt gcttactttc cggccgcggt agacgtaggt
     1741 cgttgccggt tctgctgtcg cctgttcggc cagaatctgc atctttgcat gcacaggtga
     1801 aagatcgcag cttggatcgg tcgcggcggc atcccccgtg attgccagcg cctggcagcg
     1861 gcagccgccg aaatcctgct gttgaaaggc acaggatcgg cacggttctt tcatccaggc
     1921 ggttccgcgg aatgcctcga aggcgggcga attcgcccag atatcggcca ggctatgatc
     1981 ccgcacggac cagaactcca ggccagggat cgtttgggcc gcgtggcagg gcagcaccaa
     2041 gcctgtcggg gtcacgttca acgagcgacg tccccagccg cccatgcaag gcttcgggcg
     2101 tcttgcgtag tagtcgggaa tcacggaatc gattgataat ttgccggcat accgcgcctg
     2161 aagctgatcc atctcctgca tcgcgcgatc gatctgttcg cgcgtcggca tcagcgcact
     2221 tcgattgcgc agtgcccagc cataatattg ggcatgggca atctccactc ggcccgctcc
     2281 cagggaagca gccaaatcca ccagctccgt caggtgcgcg atgttggcgc gatgaataac
     2341 ggcattgacc gtcaatgcca tcccgaggcg gacaacggcc tgcgcgaaca cctgcttgcg
     2401 ggcgtgtgcg ccatcatagc cggcgatgcg gtcggccgcc gcaggatcgg tatcctggat
     2461 ggatacctgc acatgatcca gtcccgccgc cttcagctgc cgcagcctgt cggcatcaag
     2521 cccaatcgcg gaggtgatca ggttggtgta aagcccggcg tcactggccg cttgagtaat
     2581 ttcgatcagg tcgcggcgag atgccggctc gccgcctgag agatggatct gcaggacccc
     2641 catcgccgcg gcctgctgga acaccctcgc ccacgccgcg gcatccagtt cgtcggcccg
     2701 attctcaagt tcaagcgggt tagagcagta gggacaggcc agcggacagc ggtgggtcag
     2761 ttccgccagc aaaccgagcg gggccaggcc tgtcatgcga tcagcagccg cttgccggcc
     2821 agttcagcca gcatgtcgtt gacgtcccgc gcgataacat cccggtcggc cgcataaacg
     2881 accgcgagtt cgtc
//
LOCUS       DASWQN010000882         4600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1799957, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000882 DASWQN010000000
VERSION     DASWQN010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799957"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /gene="groEL"
                     /locus_tag="VGC82_14925"
     CDS             complement(<1..1030)
                     /gene="groEL"
                     /locus_tag="VGC82_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0424609.1"
                     /translation="MAAKDVRFGGEARARMLRGVDILADAVKVTLGPKGRNVVLDKSF
                     GAPRITKDGVTVAKEIELADKFENMGAQMVREVASKTNDIAGDGTTTATVLAQSIVRE
                     GAKAVAAGMNPMDLKRGIDKAVIAVVEELKKRSKKITTPAETAQVGTISANGESDIGK
                     MIAEAMQKVGNEGVITVEEAKGITTELDVVEGMQFDRGYVSPYFITNAEKMIADMDQP
                     YILIHEKKLSGLQPMLPLLESIVQSGRPLVIIAEDVEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAILTGGTVISEDLGIKLENVTLAMLGTAKRVLIEKENTTIVE
                     GAGSTTDIA"
     gene            complement(1161..1478)
                     /gene="groES"
                     /locus_tag="VGC82_14930"
     CDS             complement(1161..1478)
                     /gene="groES"
                     /locus_tag="VGC82_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY0424610.1"
                     /translation="MSFRPLHDRVVVRRLTAEEKTAGGIIIPDTAKEKPMEGEVIAVG
                     PGARNEAGQLVALDVAAGDKILFGKWSGTEVKLDGEELLIMKESDIMGIITGTAATKK
                     KAA"
     gene            1781..2041
                     /locus_tag="VGC82_14935"
     CDS             1781..2041
                     /locus_tag="VGC82_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Usg family protein"
                     /protein_id="HEY0424611.1"
                     /translation="MTLVRQLQDYRLTTAEILYHLPDHPNLIQAFIWQDLDIAPRYPV
                     LHKFLDFWKREIEGSLHSVRVASASLVKPPTVGHAETMLRLH"
     gene            complement(2293..3243)
                     /locus_tag="VGC82_14940"
     CDS             complement(2293..3243)
                     /locus_tag="VGC82_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEY0424612.1"
                     /translation="MTRNRTLLAGITVLTLALSPGLADARAGGGSSMGSRGSMTFSAP
                     PTTNTAPYSAAPMQRSMTPQYQPAPSYGGSPMYQGQRSSGFMSGLMGGLIGAGIGGML
                     FGHGFMGGGMGGFGFLGLLLQIFLLVIAVRFLFRLFTGARSPATAGGPNIFARGGMFG
                     AMRNASMGGGSAQPRGGSSAPPPIAISPGDYQQFEQLLQGVQAAWSAHDLSGLQAMTT
                     PEMASYFAEQLSEQVSRGVRNTVTDVNLVSGDLSQAWAEQGREYATVAMRFSMIDATR
                     DSAGRVVDGSATEHTTATELWTFVRSRGGRWILSAIQQTR"
     gene            3515..>4600
                     /locus_tag="VGC82_14945"
     CDS             3515..>4600
                     /locus_tag="VGC82_14945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424613.1"
                     /translation="MSITLPPDGLQGSRLTAAVTTLVLAVGMASLDTSIANTALPTIA
                     ADVNASPADSIWVVNAYQLALVVSLLPFASLGEIIGYRRVYVWGLAVFTGASLLCALS
                     WSLPTLTMARIIQGFGASGIMSVNTALIRFIYPSGSLGRGVGNNALVVAVSTAVGPTI
                     AAGILSFGTWQWLFAVNVPFGLIAFVLALRALPDTKRAPHRFDFGSAVLNAATFGLLI
                     FGIGEATHRAGLLITVSILVAALASGITLIYRQLGLQAPMLPVDLYRRPMFALSSLTA
                     LFSFAAQGLAFVALPFYFQSVLGLSAVQTGLLLTPWPVMTAIAAPIAGPLSDRYPPAV
                     LGGIGLAILSLGLALIAALPGHPGTGGI"
BASE COUNT          750 a         1504 c         1393 g          953 t
ORIGIN      
        1 cggcgatgtc ggtcgtggaa ccggcgcctt cgacgatcgt ggtgttctcc ttctcgatca
       61 gcacccgctt cgccgtgccc agcatcgcaa gcgtgacatt ttccagcttg atgcccaggt
      121 cttcgctgat cacggtgccc ccggtcagga tcgcgatgtc ttccagcatc gccttgcggc
      181 gatcaccgaa gccaggcgcc ttcacagccg caaccttcag gccgccacgc agcttgttca
      241 ccaccagagt cgccagggcc tcgccctcga catcctcggc gatgatgacc agcggacggc
      301 cagactgcac aatgctttcc agcagcggca gcatcggctg caggccggac agcttctttt
      361 cgtggatcag gatgtacggc tgatccatat cggcgatcat cttctccgcg ttcgtgatga
      421 aatacgggga aacgtaaccg cggtcgaact gcatgccctc cacaacgtcg agttcggtcg
      481 tgatgccctt ggcttcctcg acggtgatga caccctcgtt gccgaccttc tgcatcgctt
      541 cggcgatcat cttgccgatg tcggattcgc cgttggccga gatcgtgccg acctgggcgg
      601 tttccgccgg ggtggtgatc ttcttggagc gctttttcag ctcttccacg acggcgataa
      661 ccgccttgtc gatgccgcgc ttgaggtcca tcgggttcat gccggcggca acagccttcg
      721 cgccctcacg gacaatggac tgtgccagca cagtcgcggt ggtggtgccg tcgccggcga
      781 tatcgttggt tttcgaggcc acttcgcgca ccatctgggc gcccatgttc tcgaacttgt
      841 cggccagctc gatttccttg gcgacggtca cgccgtcctt ggtaatccgc ggcgcgccga
      901 aactcttgtc cagaaccacg ttgcggccct tcgggcccaa tgtcaccttt acggcgtcag
      961 ccaatatatc cacgccacgc aacatgcgcg cgcgggcttc gccgccgaag cgaacgtcct
     1021 ttgcagccat cagtattgtt ccctcattgt cgataaccgt cgaagtcaaa ggcgtgcatt
     1081 agtgcaccga gcgtggcggc tgtgccgtcc acggcccgat gactacgacc aaaggcttcc
     1141 gtggtaagaa gaagacccgc ttatgcggcc ttcttcttgg tcgcggcggt tccggtgatg
     1201 atgcccatga tgtcggactc cttcatgatc agaagttcct caccatccag cttcacctcg
     1261 gtgccggacc acttgccgaa caggatcttg tcgcctgccg cgacgtcgag agccacaagt
     1321 tgacccgctt cgttgcgggc gccgggacct acggcgatga cttcgccttc catcggcttt
     1381 tccttggccg tatcggggat gatgattcct ccggcggtct tttcttcagc ggtcaggcgc
     1441 cggactacaa cccggtcgtg cagggggcgg aaactcattt taagatgctc ctgagttcgt
     1501 tctcagatga aatggatcgc cacaaccgaa cgcgggcttt gatgagcggc tgtcggttgt
     1561 tagcactccc aggcagggag tgccagcgat ctagccccga tgggtgccga agtcaagcgt
     1621 tggcagcact ctttcgtcaa gagtgctaag atgttgaata tgttgtgttt cctgttgcgt
     1681 ccggcggttg cgctccgaca ttgtagtgtc attgggccgg caatgtcctg gttaggcctc
     1741 gccgagtccg tccataagct tgaataggaa gcggaaagcc atgacgctcg ttcggcagtt
     1801 gcaagattat cgtctcacca cggccgaaat cctctaccat ctgccggatc atccaaacct
     1861 gatccaggcc tttatctggc aggatctcga tatcgcgcca cgctacccgg tgttgcacaa
     1921 attcctcgat ttctggaaac gagagatcga gggcagcctg cattcggtcc gcgtcgcatc
     1981 cgcgagccta gtcaaacccc ccaccgtcgg ccacgcagag acgatgctgc ggcttcacta
     2041 ggtcaggtcg cgccgctgcc cttcgggctt cgggcggggg atcgccgaac gggtcactcc
     2101 ggcccgttcg gccgcgtgaa ccaggcgttc tatcatcgca gtcacgcgcc cgtcgctcgt
     2161 gccctagctt ggctcggggc gcttcgggcg ctgctgcgtc gtcatcgcgc aggctatggc
     2221 ttcccgatcc gcgggctgcc atttcgcccg tcggatcgga tagctgttcg cccggatggc
     2281 aaccgaacgc ggttaccgag tttgttgtat cgccgacagg atccagcgcc cgccgcggga
     2341 gcggacgaag gtccagagtt cggtcgccgt ggtgtgttcg gtggcgctgc cgtcaaccac
     2401 ccgtccggcg ctgtcgcggg tcgcatcgat catcgagaac cgcatcgcga ccgtggcgta
     2461 ttcgcggcct tgctcggccc acgcctgcga caggtcgcct gacaccaggt tcacgtcggt
     2521 gacggtgttg cgcacgccgc ggctgacctg ctccgagagt tgttcggcga aatagctggc
     2581 catctcaggc gtcgtcatcg cctgcagacc gctcaggtca tgcgcgctcc aggctgcctg
     2641 aacgccttgc agcaactgtt cgaactgctg gtaatcgccg ggactgatgg cgatcggcgg
     2701 cggagcagaa gagccacccc ggggctgcgc ggagccgccg cccatcgagg cgttccgcat
     2761 tgccccaaac ataccgccgc gggcgaagat gttcgggcca cccgcggtcg ccggggatcg
     2821 ggcaccggtg aacagccgga aaagaaaccg caccgcgatg acgagcagga agatctgcaa
     2881 cagcaggccg aggaagccga agccgcccat gccgccgccc atgaagccgt ggccgaacag
     2941 catcccgccg atgccggcac cgatcaggcc gcccatcagg ccagacatga agccggagga
     3001 acgctgccct tgatacatcg gcgagccgcc gtagctgggt gccggctgat actgcggggt
     3061 catgctgcgt tgcatcggtg cggccgaata cggcgcggtg ttggtcgttg gcggcgcgga
     3121 gaacgtcatg cttccgcgac tgcccatcga actaccccca ccagcccggg cgtcggcgag
     3181 cccgggggac agagccaacg ttaggaccgt gatgccggcc aacagggttc gattgcgagt
     3241 catttctttc actttaacgt tgtgttctgc ccaatatggt gcgccgggga gactaaagcc
     3301 agtggcatcc cgcgctgcca cgaaccaact gcccacgcgt cacgttcagg cgccgtccat
     3361 cgcggagatt ttcgctgatg gcgctacgac aaatgggttt caacctgacc atgcaaaccg
     3421 aattggccct tctttatgct tgatcggctg actggcaccg ccggtacgcg gcgtcctgcc
     3481 gggacctgac ctgtcagcgg cgccggcgga cacgatgagc atcacgttac cgccagatgg
     3541 cctgcaaggc tcccggctga ccgctgccgt caccacgttg gtcctcgcgg tcggcatggc
     3601 gtcgctggat acatcgatcg ccaacaccgc tttgccaacc atcgccgcgg acgtgaatgc
     3661 cagcccggcc gactcgatct gggtagtcaa tgcctatcag ttggcattgg tggtttcgct
     3721 gctgccgttc gcctccctgg gcgagatcat cggttaccgc cgcgtctatg tctggggcct
     3781 ggcggtgttc accggcgcgt cgctgctctg cgcactgagc tggtcgctgc ccactctgac
     3841 catggcgcgg atcatccaag gcttcggcgc cagcgggatc atgagcgtca acaccgcgct
     3901 gatccggttc atctacccct cggggtcgct gggccgaggc gtcggcaaca acgcactggt
     3961 agtcgccgta tcgaccgctg tcggaccgac cattgccgcc ggaatcctgt ctttcgggac
     4021 ctggcaatgg ctgttcgcgg tcaatgtccc gttcggcctg atcgccttcg tcctggcgtt
     4081 gcgcgctttg cccgacacca agcgcgcgcc gcaccggttc gatttcggca gcgccgtgct
     4141 gaatgcggcc acgtttggcc tgctgatctt tggcatcggc gaggcgacgc accgcgccgg
     4201 cctcctgatc accgtcagca tcctcgtggc ggcgttggcg tctggtatca cgctgatata
     4261 ccggcaactc ggactccagg caccgatgct gccggtggat ctttaccgcc ggccgatgtt
     4321 cgccttgtcc tcgctgacgg ccctgttctc gttcgcagca caggggttgg cgttcgtcgc
     4381 cctgcccttc tacttccaga gcgtgctggg gcttagcgcc gtccagaccg gattgctgct
     4441 gaccccttgg ccggtcatga cggcaatagc ggcacccatt gccgggccgc tgtccgaccg
     4501 ctacccacca gcggtgctcg gcggcatcgg tctcgcaatt ttaagcctgg ggctggcttt
     4561 gattgcggca ttgcctggtc atcccggaac cggcggcatc
//
LOCUS       DASWQN010000883         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_876565, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000883 DASWQN010000000
VERSION     DASWQN010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876565"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VGC82_14950"
     CDS             complement(<1..458)
                     /locus_tag="VGC82_14950"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEY0424614.1"
                     /translation="MTRKLVSQANVLVFAAIIGFLALIGFTTWDRFVAARSARDWTTH
                     TYAVLAAAGKVEIAVRDAETGQRGYLLTGDKAYLEPYESALGQMPDLLADLQRLTADN
                     AVQQARLSLLAPAWQRRSQQFVQNIQAREDKGLSAAAAILQTGGGRETMT"
     gene            633..1370
                     /locus_tag="VGC82_14955"
     CDS             633..1370
                     /locus_tag="VGC82_14955"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY0424615.1"
                     /translation="MARLSVNLNKIALLRNSRRTGVPDLRRFGRLALEAGAEGLTVHP
                     RPDERHIRGSDVPMLAELMRPSRPGKELNIEGYPDERLMQILRENRPEQATLVPDAPD
                     AFTSEEGWKLDPDQARLAAPAIAAMKALGCRVILFIDPDPDVIDRVRAAGADGVEIYT
                     GAYAAAFRDGRQQPILEQIGATAVRAKTAGLVVNAGHDLNLRNIPLLMRAVPFLAEAS
                     IGHELTADALERGFTATVAAYAQALSG"
     gene            complement(1377..>2014)
                     /locus_tag="VGC82_14960"
     CDS             complement(1377..>2014)
                     /locus_tag="VGC82_14960"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0424616.1"
                     /translation="AALLRPDRFRAVIGLSVPYRPRHKVRPTTMMPQTDDAVFYQLHF
                     QAPGVAEAELEEDVRASIRAIVLANSGEAPGGFGMVPRAGGLRSGLRAAGSAALPGWL
                     TETDLDFYVGEFNRTGFRGGLNWYRNIDRNWEFLAPFAGAKVAVPALYVVGERDLVLT
                     FPGAKELAANLRTFVPRLQETIMLPGCGHWTQQERPDAVNAAMIGFLRGLA"
BASE COUNT          369 a          667 c          629 g          349 t
ORIGIN      
        1 tgcgtcatcg tttcgcgtcc accgccggtc tgcaggattg cggcagccgc gctcaagccc
       61 ttgtcctcgc gcgcctggat gttttgcacg aactgctgcg agcggcgctg ccaggctggc
      121 gccagcaaac taaggcgggc ctgttggaca gcattgtcgg cggtgagtcg ctgcaaatcc
      181 gcaagcaggt cgggcatctg accgagagcc gattcgtacg gctccagata ggccttgtcg
      241 ccggtcagca gatagccgcg ttgcccggtt tcggcgtcac gaaccgcgat ttcaaccttc
      301 ccggccgctg ccaggacggc ataggtatgg gtggtccaat cccgtgcgga gcgcgcggca
      361 acaaaacggt cccatgtcgt gaagccgatc agcgccagga aaccgatgat ggcggcgaac
      421 accaggacgt tggcctggga gacgagtttc ctggtcacga cgcgcccaac ccccgatgcg
      481 gatgcagaat tcccctcctg aacccggttc gttcgggccg gggatcgcct tcgggtatgt
      541 ttagtaagca tcccaaaaag tctattcacg ctcgggtgcg gggtacggac gccatgttga
      601 gtcaccgatg atgacgggtt acgctccatt gtatggcacg acttagcgta aacttgaaca
      661 aaatcgccct gctgcggaat tcccgccgca ccggcgtacc cgacctgcgc cgtttcggtc
      721 ggttggcgtt ggaagccgga gccgagggat tgaccgtgca tcccaggccg gatgagcgcc
      781 atattcgcgg cagcgacgtg ccgatgctgg cggagctgat gcggccctcg cggccgggca
      841 aggaactcaa catcgagggc tatccggacg agcggctgat gcagatcctg cgcgaaaatc
      901 ggccggagca ggcgacgctg gtgccggatg cgccggacgc attcacctcg gaggaaggct
      961 ggaagctgga tcccgatcag gcccggctgg cggcccccgc catcgccgcg atgaaagccc
     1021 tcggctgtcg ggtgatcctg ttcatcgatc ccgatccgga cgtgatcgat cgggtacgcg
     1081 cggcgggagc cgatggcgtg gagatctaca ctggcgccta tgccgcggcg tttcgcgatg
     1141 gccggcagca gccgatcctg gagcagatcg gtgcgacggc cgtccgtgcc aaaaccgcgg
     1201 gcttggtcgt caatgccggg cacgacctga acctgcgcaa tattcccttg ctgatgcgcg
     1261 cggtgccatt cctcgcggag gcctcgatcg gccacgaact gacggcggac gcgctggagc
     1321 gcggtttcac ggcaacggtc gcggcctatg cgcaggcatt gtcgggctaa agcctttcac
     1381 gccagtccgc gcaaaaaccc gatcatcgcg gcgttcaccg catccggccg ctcctgctgg
     1441 gtccagtggc cacaccccgg cagcatgatc gtttcctgaa gtcgcggcac gaaggtccgt
     1501 aaattcgccg ccagttcctt tgcgcccgga aacgtcaata ccagatcccg ctcccccacg
     1561 acatacagcg ccggcacggc aaccttcgcc cccgcgaacg gcgcgaggaa ctcccagttc
     1621 cggtcgatat tgcgatacca attaagaccg ccgcgaaacc cggtgcggtt gaactcgccc
     1681 acatagaaat cgagatccgt ttcggtcagc caccccggga gcgccgccga accggcggct
     1741 cgcagtcccg accgcagccc gcctgccctc ggcaccatgc caaagccgcc tggtgcctcc
     1801 cctgaatttg ccaatacgat tgctcggatc gaggctcgaa catcctcctc cagctccgcc
     1861 tcggccaccc ccggagcctg aaagtgcaac tgatagaaca ccgcgtcgtc ggtctggggc
     1921 atcatggtag tgggacgcac tttgtgccgt ggccgatacg gcacgctcag tccgatcacc
     1981 gcgcgaaacc ggtccggtcg cagcaaagcc gcat
//
LOCUS       DASWQN010000884         8294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_876671, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000884 DASWQN010000000
VERSION     DASWQN010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876671"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VGC82_14965"
     CDS             <1..967
                     /locus_tag="VGC82_14965"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEY0424617.1"
                     /translation="AAGAKTAAANGDSVILGPLTSAETAAVAPIAREAGIPVLAFTND
                     ASQSQPGVWPLGITPDQQIQRLVGSAQAQGRAQFAGLLPDSDFGRAMATALTRATTAA
                     GLPPPNIRMHSPGMAAITASVRDLSDYANRRGPIDAKVKAARALGTPDGRRTAQELVR
                     TPILPPSFNVLLLADTGDNLQEIAAVLPYYDVDHSMVQVIGPALWADPASGSGAVRGA
                     WYAAPDSNSRSNFERDYAAKYGSRPPPLADLAFDSALIAKVAAGSAGGNLQVLTQPGG
                     FVGTDGWLILLPDGRVRRGLAVFRIERSGPELIDPAPQSSTEPVL"
     gene            complement(1043..2050)
                     /gene="trpS"
                     /locus_tag="VGC82_14970"
     CDS             complement(1043..2050)
                     /gene="trpS"
                     /locus_tag="VGC82_14970"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY0424618.1"
                     /translation="MQRVFSGIQPSGIPTLGNYLGAIRNWVTLQDDHECLWCIVDLHA
                     ITVWQDPADLRRQTLEMAAVLLACGVDPKNHVLFPQSGVRAHAQLAWIFNCVARLGWL
                     NRMTQFKDKAGKDRENASSGLYVYPNLMAADIHAYHSTRVPVGDDQRQHLELANDIAQ
                     KFNHDYGADFFPNIEPVILGPAARVMSLRDGTKKMSKSDPSDQSRINLNDDADTIALK
                     IRRAKTDPMPLPSEVEGLADRPDARNLVGIYAALTDTDLAAVLREHGGQGFGPFKSAL
                     TEVLVEKLAPIASETSRRLGDQEALIDLLRSGAERAAAIADPIVSEAERLVGFIGIRG
                     G"
     gene            complement(2055..3644)
                     /gene="murJ"
                     /locus_tag="VGC82_14975"
     CDS             complement(2055..3644)
                     /gene="murJ"
                     /locus_tag="VGC82_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY0424619.1"
                     /translation="MLRGILTVGGWTLVSRILGFGRDMLIAALIGTGPIADAFFVALK
                     LPNLFRRLFGEGAFNAAFIPAFSGLLHTEGVGAARQFAQEAFAVMAFWLGILTILGEI
                     FMPQLMVVLAPGFAEDPGKFALAVSLSRITFPYLVLICLAALVSGVLNGLEKFTAASA
                     SYVLFNIVSIACMIWMTPYVPTVGHALSWGVTISGVAQLGLLMLAVRRGGMALKIPRP
                     RMTPQMRLLLGRMAPGLVGAGVTQLNLTVDVVIASLLPPGSVSVLYFADRVQQLPLGV
                     IGTAVGTALLPLLSRQVRAGEAASANGTLNRAVEYGLFLTLPAALALIVCAYPIMLVL
                     FGRGAFDLESVLLSSQSLVAYALGLPAFVLVKVFAPAFFARGDTSMPVKIGIGSVTLN
                     LALNLAFMVPLAHIGPALATSLAAMCNVGGLALVLVRRGHFVPDRQLWKRCLGMIGAS
                     VAMAAVLWEARRLLFAGPTHGWQGLASLGGLVGAGMLAYAVAAPLLGAYDLRDIGRMM
                     SRRRLRTESGSAISRAPTTET"
     gene            complement(3734..4696)
                     /locus_tag="VGC82_14980"
     CDS             complement(3734..4696)
                     /locus_tag="VGC82_14980"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY0424620.1"
                     /translation="MSKVAVIGAGAWGTALALQAVRADHRVVLVARDAASASAMEHTR
                     ESPRLPGVRLPQQLDVRDDIPHDAELMLWVVPTQHLRSSLSRLRTASGMIVVCAKGVE
                     AGTHLLPLEVVSDVVPSRPLAILTGPNFAQEVADGLPTASVVASADATIRERVLSMLG
                     TATFRLYGNDDPIGAQLGGAAKNVIAIAAGAVIGAGLGENARAALVTRGLRELCRLAV
                     ALGGRADTVMGLSGLGDLLLTCTGPSSRNFSLGYALGQGQSLASILAERMAVTEGVPT
                     AAALVARAGQVELPICRAVADLLAGVKTLDQAITQLLSRPQRDE"
     gene            complement(4689..5507)
                     /gene="rlmJ"
                     /locus_tag="VGC82_14985"
     CDS             complement(4689..5507)
                     /gene="rlmJ"
                     /locus_tag="VGC82_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEY0424621.1"
                     /translation="MNYRHAYHAGNFADCFKHALLIALLDSFVRKPAPFFVLDTHAGA
                     GRYDLDAEPSRRTAETDGGIGRLLRSPSPALARYLGLVTELGLYPGSPTFIRAVLRAE
                     DRLVCCERHPEDVTLLRRLFFRDRQVEVHDRSGWEALTGLLPPREKRGLVFIDPPFEA
                     KDEFFTLTDELQRGHDRFGHGVFAAWYPIKQMAAVRGVHARLKSSGMRDIVAVELHLR
                     DPSSAERLNGCGLVVINPPYQFEDQAGLIARDVLDGVGENEVGAGTKVLRLVDE"
     gene            complement(5507..6556)
                     /gene="tsaD"
                     /locus_tag="VGC82_14990"
     CDS             complement(5507..6556)
                     /gene="tsaD"
                     /locus_tag="VGC82_14990"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY0424622.1"
                     /translation="MKPVLGIETSCDETAASVVDGEGHVLAETVLSQLAEHAPFGGVV
                     PEIAARAHLRYLSEQVRRTMAQAGVAFADLGGVAATSGPGLIGGLIVGSQFGKGVAIA
                     NGLPLVSVNHLEAHALTARLPGLVEGGAPFPYLLFLLSGGHSQCIAVMGIGRYVRLGG
                     TVDDAAGEAFDKVAKLLGLDWPGGPALEKTALGGDPMRFPLPRPMMRRPGCDLSFSGL
                     KTAVAQLVPRLGPRLTDEQRADIAASFQLAVVEVLADRAGHAMGMMHERFPSADLLVV
                     AGGVASNAAVRAGLGKAALSQGFRLVAPPARLCSDNAVMVAWAGVERLRLGWSDGLGA
                     DARPRWPLEALEAVS"
     gene            6677..7420
                     /locus_tag="VGC82_14995"
     CDS             6677..7420
                     /locus_tag="VGC82_14995"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY0424623.1"
                     /translation="MLPVQIIGRRFIDKSSVLKRILITRPEPGASQTAARLTALGLMG
                     VVAPVLSITTRPLRAPDRMAATILTSRNAVPSCPPSIHDRPVFAVGTATAAMALEAGF
                     KQVLDADGDAIALANLIAETLKPADGALFLPTGLGQGADLAASLRQHGFRVLRRVAYH
                     AASVPILPDVAATHLRQGGLVAAMFFSGDTSHHFVRLLRAANLTESVRDVEAVSISER
                     AAMPLRPLPWRRISVAPKPNQDAMLALLK"
     gene            7417..>8294
                     /locus_tag="VGC82_15000"
     CDS             7417..>8294
                     /locus_tag="VGC82_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424624.1"
                     /translation="MIEQDPPLPPDGPPEATAVAPKPRRRDLVPWFYGLGFLVLAGAL
                     FYLWQNPTIPGEPAASVSARQAVEQRLNDIDSRLSRLEQRPVPDLGPITARVDALGGR
                     IADQTQLGARLDAVSGRIESLSGRQQTGLDAAKQQIDSLASRIAAIETNAGGLDAVTK
                     RLNRIARLQEASLALASGRPIGDLPNAPEALARYARTAPPTEAQLRLSFPRAEQAALA
                     AQQPDKKSAPFIERVWDRAQGLVTVRRGDDLVVGNSSATTLNQAQTALDAGDLAGAVA
                     AVETLEGPPAEAMAAWL"
BASE COUNT         1667 a         2923 c         2386 g         1318 t
ORIGIN      
        1 cgcggccggg gcaaaaacgg ccgccgccaa cggcgacagc gtgatactcg gtccgctgac
       61 gtccgccgaa actgccgcgg tcgcgccgat cgcccgcgaa gcgggaatac cggttttggc
      121 gttcaccaac gacgcgtcgc agtcccaacc cggtgtttgg ccccttggca tcacgcccga
      181 tcaacagatc cagcgtttgg tcggcagcgc tcaggctcag ggaagagcgc agttcgctgg
      241 cctgctgccg gattccgatt tcggccgggc aatggccacc gccctgacgc gggcgaccac
      301 cgccgcgggc ctgccaccgc cgaacattcg gatgcattca cctggaatgg cagcgattac
      361 cgcctcggtc agggacttgt cggactacgc caaccgccgg ggaccaatcg acgcaaaggt
      421 taaggccgcc agggctttgg gaaccccgga cggacgccgt accgcccagg agcttgtaag
      481 aacgcccatc ctgccgcctt cgttcaatgt tctgctcctg gcggatacag gtgacaatct
      541 gcaggagatc gccgcggtat tgccctacta cgatgtggac cactccatgg ttcaggtcat
      601 cggaccggca ctgtgggctg atccggcaag tggttcgggt gcggtccggg gcgcctggta
      661 tgcagcgccc gacagcaatt cgcgcagcaa cttcgaacgc gattacgccg ccaagtatgg
      721 cagccgaccc ccgccgttgg ccgatctggc cttcgattca gccctgatcg caaaagtggc
      781 tgccggatcg gcaggcggca atctgcaggt cctgacgcag cccggcggct tcgtgggaac
      841 cgacggctgg ctcattctgc tccccgatgg ccgggtacgg cgcggtttgg cagtgttccg
      901 tatcgagcgc tccggtcccg aactgatcga tcccgcgcca cagtcgtcca ccgaaccagt
      961 gctgtgaacg gctaatatca tgtgtcatgg ccgtgcttgg tacggccacc cacaactttc
     1021 tttgcaaccg gtcggcataa cctcagcctc cacgaattcc gataaagccg accagccgct
     1081 ccgcctcgct gacaatcgga tcggcgatcg cggctgcgcg ttcggcgccc gaacgcagaa
     1141 gatcgatcaa cgcctcctgg tcacccaacc tccggctggt ctccgaggcg atcggcgcca
     1201 gtttttccac cagcacttcc gtcagcgcac tcttgaacgg tccaaaaccc tgccccccgt
     1261 gctcgcgcag caccgcggca agatcggtat ccgtcaaggc ggcatagatc ccgaccaagt
     1321 tccgggcatc cggccggtca gccagccctt cgacctcgga cggcaacggc atcgggtcgg
     1381 tcttcgcccg cctgatcttc agcgcgatcg tgtcggcatc gtcattgagg ttgatgcggc
     1441 tctgatcgga cgggtccgac ttggacatct tcttcgtccc atcccgcaga ctcatcaccc
     1501 gtgcagcagg gcccaggatg accggctcga tattcggaaa gaaatccgcc ccgtagtcat
     1561 gattgaattt ctgcgcgata tcgttcgcga gttccaaatg ttgccgctga tcgtcgccaa
     1621 ccggcacccg ggtcgagtgg tacgcatgaa tgtccgccgc catcaggttc ggatagacat
     1681 atagcccgga cgacgcgttc tccctgtcct tgcccgcttt gtccttgaac tgagtcatgc
     1741 ggttcagcca gccgaggcgg gcgacacagt tgaagatcca ggcaagctgg gcgtgggcac
     1801 gcacgcccga ctgcggaaac aggacatggt tcttcggatc gacgccgcag gccagcagga
     1861 cagccgccat ttccagcgtt tgtcgccgca ggtccgccgg atcctgccac accgtgatgg
     1921 cgtgcaggtc cacgatacac caaaggcact cgtgatcatc ctgaagcgtg acccagttgc
     1981 ggatcgcacc aaggtagttc cccagtgtgg gaatgccgga cggttggatg cccgaaaaga
     2041 cgcgctgcat acgttcaagt ctctgtcgtt ggggcccgac tgatggctga tccgctctcg
     2101 gtgcgcaagc gacgccgcga catcatgcgt ccgatatcac ggagatcgta ggcacccagc
     2161 agcggcgccg caacggcgta ggcaagcatt ccggcgccga ccagtccccc gagagacgcc
     2221 aaaccctgcc agccgtgcgt cgggcctgca aacagcaatc gccgagcttc ccacaatacg
     2281 gcagccatcg ccaccgatgc cccgatcatg ccgaggcagc gcttccagag ctggcgatcc
     2341 ggtacgaaat gtccccgccg aaccaggacc aacgccagtc cgccgacatt gcacatcgcg
     2401 gccaggctcg tcgccagcgc cggtccgata tgcgccaacg gcaccatgaa ggccaggttc
     2461 aaggccaggt tcaaggtgac acttccgatg ccgatcttga ccggcatgct ggtgtcgcca
     2521 cgggcaaaaa acgccggtgc aaagaccttc accagcacaa aggccggcag cccgagtgca
     2581 taggccacca gcgactgcga tgacagaagc acgctctcca ggtcgaatgc accacgcccg
     2641 aacagcacca gcatgatcgg atacgcgcaa acgatcaggg ccaaggccgc cggcaatgtc
     2701 agaaatagcc cgtactcgac ggcgcggttc aacgtgccgt tggccgaagc ggcctctccg
     2761 gcacggacct ggcgggacaa cagcggcaac agcgccgttc cgacggccgt gccgatcacc
     2821 ccgagtggca attgctggac ccggtccgcg aagtacagca ccgagacgct gccgggcggc
     2881 aaaaggctgg cgatgaccac atcgaccgtc aggttcagtt gggtaacccc cgctccgaca
     2941 agtccgggcg ccattctgcc caacagcaga cgcatctgcg gcgtcatcct cgggcgggga
     3001 attttcagcg ccatcccgcc acgccgcacc gccaacatca gcaaaccaag ctgagcgacg
     3061 cccgagatcg tcacgcccca tgacagcgcg tgccccactg tcgggacgta aggcgtcatc
     3121 cagatcatgc aggcgatcga gacgatgttg aacagcacgt acgatgccga cgccgcggta
     3181 aatttttcca gcccgttcag aacgcccgaa accaaagcgg cgaggcagat cagcacgaga
     3241 tagggaaagg tgattctgga caggctgacg gcgagggcga acttgccagg gtcttccgcg
     3301 aaccccgggg ccaggaccac catgagctgg ggcatgaata tttcgcccag gatcgtcagg
     3361 atcccgagcc aaaacgccat gaccgcgaag gcctcctggg caaactgccg cgccgcccca
     3421 acgccttccg tgtgcaacag tcccgagaac gcagggatga acgccgcgtt aaaagcaccc
     3481 tcgccgaaca accggcgaaa caggttgggc agcttcaagg ccacaaagaa cgcatcggcg
     3541 atcggcccgg tcccgatcag cgccgcgatc agcatgtccc tgccaaatcc caggatccga
     3601 ctgaccagcg tccacccgcc gacggtgaga atacctctca gcacgtcagt tcttgctccg
     3661 ctttgcgagc cgcggggaat cgcacagcgt cacatggggg cgaaatatgc ccgtacgaaa
     3721 caccgatcca catctattca tcccgttgcg gacgcgacag aagttgcgtg attgcctgat
     3781 ccagcgtctt cacgccggcc agcaggtccg cgaccgcgcg gcagatcggc agctccacct
     3841 gtcccgcacg ggcaaccagc gcggcggccg tcggaactcc ctcggtcacc gccatccgct
     3901 cggccagaat gcttgccaga ctctgcccct ggccaagcgc gtaccccagg ctgaaattcc
     3961 ggctcgacgg ccccgtgcaa gtcagcagca ggtcaccgag accggacaaa cccataacgg
     4021 tgtcggctcg tccccccagc gcgaccgcca gacggcacag ttccctcagc ccccgggtca
     4081 ccaaggctgc tcgggcgttc tcccccagtc cggcgccaat caccgcgccg gccgcaatcg
     4141 cgatgacatt cttcgcggcc cctccaagct gcgccccgat tggatcgtca ttcccataca
     4201 aacggaaggt ggcggtcccc aacatcgaca gcacacgctc ccgaatcgtg gcatccgcac
     4261 tggccaccac cgacgccgtc ggaagcccgt cggccacctc ctgcgcgaaa ttgggcccag
     4321 tcagaatggc caacgggcgg gaagggacca catcgctgac cacttcgagt ggaagcagat
     4381 gtgtccccgc ttcgaccccc tttgcacaaa cgacgatcat cccggatgcg gttcgcaggc
     4441 gcgaaagcga agaccgcagg tgctgcgtcg gcaccaccca caacatcagc tcagcgtcat
     4501 gggggatgtc gtctcgcacg tccagttgct gtggaagccg cacgccaggc aaccgcgggc
     4561 tttccctggt gtgctccatg gcagaggcag aagccgcatc gcgtgccacc agcacgaccc
     4621 gatgatccgc acgcaccgcc tgaagtgcca acgccgttcc ccacgcgccg gcaccgataa
     4681 ccgcgacctt actcatccac cagtcttagc actttggttc ccgcaccgac ctcattttcg
     4741 ccaaccccgt ccaggacatc gcgggcgatc aggcccgcct ggtcctcgaa ctgataaggc
     4801 ggattgatca ccaccagccc gcatccattg agccgttcgg cactagacgg atcacgcagg
     4861 tgaagctcga ccgcgacaat gtcccgcatc ccggacgact tcagccgcgc atgaacgccc
     4921 cgaaccgcgg ccatttgctt gatcgggtac cacgcggcaa acaccccatg gccaaatcga
     4981 tcatgaccgc gctggagttc gtccgtgagt gtgaagaact cgtctttcgc ctcgaacggc
     5041 gggtcgatga acaccagccc gcgcttttcc cttggcggca acagcccggt caacgcttcc
     5101 catccggatc ggtcatgcac ctccacttgc cggtcgcgga aaaacaggcg ccgcaggagc
     5161 gtcacgtcct caggatggcg ttcgcagcaa accaaccgat cctccgccct cagcaccgca
     5221 cggataaagg tcggcgatcc cgggtagagg ccaagttcgg ttaccaaccc cagatagcgc
     5281 gccagcgccg gcgacggcga gcgcagcaag cgcccaatgc caccatccgt ctcggctgtc
     5341 cgtcgagacg gttcggcatc gaggtcataa cgcccagccc ccgcatgcgt gtccaaaacg
     5401 aaaaatggtg caggcttgcg cacgaacgaa tcgagaaggg cgatcagcag cgcgtgcttg
     5461 aagcagtccg cgaaatttcc ggcgtgataa gcgtggcgat aattcatcag gaaaccgctt
     5521 ccaacgcctc cagcggccac cgcgggcggg cgtcggcacc aaggccgtcg ctccagccga
     5581 ggcgcaatcg ttccacaccg gcccatgcaa ccatgacggc gttgtcggaa cacagtctgg
     5641 caggcggagc aaccaggcgg aacccctgcg acagagccgc tttacccaac ccggcccgca
     5701 cagccgcgtt cgatgcgacc ccgccggcca ccaccagcag gtcagccgac ggaaaccgct
     5761 catgcatcat ccccatcgca tgaccagccc gatccgccag gacctcgaca acggcaagct
     5821 ggaagctggc ggcgatgtcc gcgcgctgct catcggtcag acgaggaccc agccgcggca
     5881 ccagttgcgc caccgctgtc ttcaagcccg aaaaactcag atcgcatccc ggccgccgca
     5941 tcatcggacg cggcaacgga aaccgcattg gatcgccacc aagagctgtc ttctccagag
     6001 ccggcccgcc tggccaatcc agccccaaca gcttggcgac cttgtcgaac gcctcgcccg
     6061 cggcatcatc gaccgttccg ccaagccgga cataccggcc aatgcccata acggcgatgc
     6121 actgagaatg tccgccggaa agcaaaaaca acaggtacgg aaatggcgcc ccgccttcga
     6181 ccagcccggg caaccgagcc gtaagcgcgt gcgcctccag gtgattgacg cttacaagcg
     6241 gcagaccgtt ggcgatcgca acacccttgc cgaattggct tccaacgatc agcccaccga
     6301 tcagccccgg tcccgacgtt gccgccaccc cgccaagatc ggcaaatgcg acgccggcct
     6361 gggccatcgt ccggcgcact tgttcggaaa ggtatcgcaa atgcgcacgc gccgcgattt
     6421 cgggaacgac accgccaaat ggcgcatgct ccgccagttg gctcagcacg gtctcggcca
     6481 ggacatgccc ctcgccatcg accacggaag cggccgtttc atcgcaggat gtctcgatcc
     6541 ccagaaccgg tttcatcgca tcgttcccac caacgtccgg ctccccaagg tccggctccc
     6601 caaggtccgg ctccaagaca taccgtcgcg gcctttgtcc agtggcgacg catcaaaaac
     6661 taggttaacg acgggcatgc tcccggttca aatcatcggc cggaggttca ttgacaagag
     6721 cagcgtgttg aagcgcatat tgatcacaag accggagccg ggcgccagcc aaaccgccgc
     6781 ccgcctgacc gcgctcggcc tgatgggcgt cgtcgcgccc gtcttatcga tcaccacgag
     6841 gccactccgg gcccccgatc gcatggcggc cacgatcctg acgagtcgaa acgccgttcc
     6901 gtcatgccct ccctccatac atgatcgtcc ggttttcgcc gtaggaaccg cgacggcggc
     6961 catggcactg gaagccggct tcaagcaggt gctcgatgcg gatggcgacg cgatcgcgct
     7021 cgccaatctc atcgcggaga ccctgaaacc ggcggatggc gccttgtttc tgccaaccgg
     7081 gttagggcag ggagccgatc tcgcggcctc gctccggcaa catggttttc gcgtccttcg
     7141 tcgcgtcgcc tatcatgcgg cgagcgtccc catccttccg gacgtcgccg cgacccatct
     7201 gcgccagggc ggcctcgtgg cagcaatgtt cttttccggg gacacttctc accatttcgt
     7261 gcgtcttctc cgggcagcga acctaaccga atccgtccga gatgtcgaag cggtgtctat
     7321 cagcgagcgg gcggccatgc cattaagacc ccttccttgg cggcgcatca gcgtcgcccc
     7381 caagcctaac caggatgcga tgctggcgtt gctgaaatga tcgaacaaga ccctccgcta
     7441 ccaccagacg gaccgcccga ggctaccgcc gtcgcaccaa aaccccggcg acgcgacctc
     7501 gtcccgtggt tttacggcct tggtttcctc gtactggcag gtgcgctctt ctacctttgg
     7561 caaaacccca ccataccggg ggaaccggcc gccagcgtat ccgcccgtca ggcagtcgaa
     7621 caacgcctca acgacatcga ttcccgcctc agccgcttgg agcaacggcc tgttcccgat
     7681 ctagggccga tcaccgcgcg ggtggacgcc ctcggcggac ggatagccga ccaaacacaa
     7741 cttggcgccc ggctagacgc ggtgtcaggc aggatcgagt cactgtccgg ccgccagcag
     7801 accggcctcg acgcggccaa acagcagatc gactcgcttg ccagccggat cgcagccatc
     7861 gaaacgaacg ccggtggcct cgacgccgtg accaaacgac tgaaccgcat cgccaggctg
     7921 caggaagcgt ctctcgcgct ggcttccggc cggcccatag gcgatctgcc caacgcgccc
     7981 gaagcactcg ctcgttatgc ccgcaccgcg ccgccaaccg aggcgcaatt gcgcctgagc
     8041 ttcccgcgtg ccgaacaggc agcactagcc gcgcaacagc cggacaagaa aagcgcgccg
     8101 ttcatcgagc gtgtctggga ccgggcgcaa ggcttggtca ccgttcgccg cggcgatgat
     8161 cttgtggtcg gcaattcctc cgccacgacc ttgaatcaag cccaaaccgc gctggacgcg
     8221 ggtgacttgg ccggcgcggt cgcagctgtc gaaaccctgg aaggaccacc tgccgaagca
     8281 atggccgcgt ggct
//
LOCUS       DASWQN010000885         4231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2196014, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000885 DASWQN010000000
VERSION     DASWQN010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196014"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..801)
                     /locus_tag="VGC82_15005"
     CDS             complement(190..801)
                     /locus_tag="VGC82_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424625.1"
                     /translation="MQERVLACTPCHGVQGGGTNNDYFPRLAGKPSGYLFNQLVAFRD
                     GRRKYAPMNYLLEYQPDAYLQRMADHFAAQRAPLPRSPVSDVSREVLARGQSLATQGD
                     PTSGVPACAGCHNPSLTGMEPGIPGLVGLRPNYITAQLGAWRYGTRTAAAPDCMQIVA
                     GHLTEPDVAAAAAYLSSLPIPADPVPVARGTLAMPLACGSEPQ"
     gene            1270..2721
                     /locus_tag="VGC82_15010"
     CDS             1270..2721
                     /locus_tag="VGC82_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0424626.1"
                     /translation="MIRQPDPKPDVMARRDSIVAGLRALLPESGLIAEKLRLKPYETD
                     GLSAYRQVPLAVVLPETTEQVAAILRFCHKNGVRVIPRGAGTSLSGGALPLADAVVVG
                     MMRMNQILDIDYPDRCAVVQAGVTNIGITNAVSADGFFYAPDPSSQLACMIGGNVNMN
                     SGGAHCLRYGVTANNLLGLKIVTIEGDIIDIGGAHLDAAGYDWLGLLTGSEGMLAIIT
                     EVTVRILRSPEGARAMLAAFKSNEIAGACVDSIISSGIIPVALEFMDRPAIHACEAFA
                     HAGYPLDAEAMLIIEVEGSVEEQDRLLGQLSAICEKFDPISLRVSQSEAESLAIWKGR
                     KGAFGAVGRISPDYLCMDGTIPTSQLPLVLRRIQEMSDSYGLKVANIFHAGDGNLHPL
                     IMFDANNPASFHAAEEFGADILKLCVEVGGCLTGEHGVGIEKRDLMPVQFHAHELQQQ
                     RDLKSAFDPDWLLNPSKVFPLLEPKAGALPHAA"
     gene            2718..3908
                     /locus_tag="VGC82_15015"
     CDS             2718..3908
                     /locus_tag="VGC82_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0424627.1"
                     /translation="MTSEDAIVATVAEAVRSREPLLVQGNGTKAGMLRPVQAARTLSA
                     SGLSGINLYAPKELILSAWAGTPLVEIEAALAEGGQHLIAEPPDLSGLLGATGKPQTF
                     GGVVATNLSGPRRVAWGAMRDHVMGIRAVTGRAEVIRSGGRVLKNVTGLDLCKLLTGS
                     HGTLGVITEITVKVLPAPEATGTLVLPGLTAAAGVAAMSAALGSPFGVSGATWLPAEA
                     VARVPGLAAVSGTATLIRIEEFAPSVTYRIGRLNDQFGVSGTITLDTAASRAVWKDVR
                     DAKPLAARAEDAVWRVSVRPSAGPSVLAALQPAGVSGFLDWGGGLVWLAGPADTATHD
                     AVEASAKAARGTWTLLRAPDTLRGAVRVVPEETAPLARITRQVKAAMDPAGILNPGRL
                     YAGL"
     gene            3911..4183
                     /locus_tag="VGC82_15020"
     CDS             3911..4183
                     /locus_tag="VGC82_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424628.1"
                     /translation="MFFIPGWISSVLFLITCYLAGYQHVQLRRPNPILNLQFLMIVFS
                     LLMILVGTVYNRTDQWLSLAFFLIAAGGLIIMIRQHRMLPPNKNFE"
BASE COUNT          737 a         1404 c         1324 g          766 t
ORIGIN      
        1 acaggcggct gccaggattt gtgtggcagg cgatgcagtc accggcgcga gcgagatact
       61 cgccctgcgt gaccatggcc gcggactgtc cgcccggcgc atcgtcggct cggacactgg
      121 agccgctggc gacgatgcag ccaatcaaaa gaacgacgcg gatgatggtc tttcgcatcg
      181 tctggtgcct cattgcggtt cgctgccgca ggcaagcggc atggcgagtg tgcctcgcgc
      241 aaccggcacc ggatcggctg ggatcggcag cgacgagaga tacgccgctg ccgcggctac
      301 gtcgggttcg gtcaggtgtc cggcaacgat ctgcatgcag tcgggcgcgg cggcggtgcg
      361 cgttccatac cgccaggcgc cgagttgcgc ggtaatgtag ttcggccgca ggccaaccag
      421 gcccggaata cccggctcca tgccggtaag ggagggattg tggcaaccag cgcaggccgg
      481 gacgccactt gtgggatcgc cttgggtagc gagcgactgc ccccgagcca aaacttcgcg
      541 gctgacatcc gaaaccggac tccgcggcag aggcgcgcgc tgcgccgcga agtggtccgc
      601 catccgctgc agataggcat caggctgata ctcgagcagg taattcatcg gggcatattt
      661 gcgccgcccg tctcggaacg cgacgagttg attgaacaag taacccgagg gctttcccgc
      721 aaggcgcgga aagtaatcgt tattggtgcc gccgccctgt actccgtggc acggtgtgca
      781 agcaagaacc cgttcttgca tgctgtcttc cacggcaggt tgcgcctttg gcggacgaga
      841 gaaaccgacc gccaagctga tcaccagcgc ggttcgcgcc aacccatacg aaaaagcgcg
      901 gaccattgtc ggaattgacg cacgcctccg acgaccgaag cgggtttcgt gaacaatcgg
      961 cggacatccg gccttatcca actgcgcccc actttatccg tcctcatgca cgcgatcatc
     1021 cgcgtccgct acgaccgcat gccacgctcg ttgtctgcgt tacgctcgtt agttcgagtg
     1081 accaaccagc aatgtgctgt ggttgttccg aagttgaaat tcaccacagc cggcgaatct
     1141 caggttcggg acgccatcgc tgtggtcggt gacctggcga tcgcccccaa cgtaagacag
     1201 cccctgtgcg ccagggtggg acacagctat ataacgacca cattttccag ccgaggaagg
     1261 gttttcgccg tgatccgtca accggacccc aagcccgacg tgatggcccg ccgcgacagc
     1321 atcgtcgccg gcctgcgcgc gctgctgccc gagagcggct tgatcgccga aaagctgcgc
     1381 ctgaagccgt acgaaaccga cgggctgtca gcttaccgcc aggtcccgct tgcggtcgta
     1441 ttgccggaaa ccacggaaca ggtcgccgcc atcctgcgct tctgccataa aaacggcgtc
     1501 cgcgtcattc cccgcggcgc cggcacctcg ctgtccggcg gcgcgcttcc gctggccgat
     1561 gccgtcgtcg tcggcatgat gcggatgaac cagatcctcg acatcgacta tcccgaccgc
     1621 tgcgccgtcg tgcaggccgg ggtcaccaat atcggcatca ccaatgccgt ctccgcggat
     1681 ggcttcttct atgcccccga cccgtccagc cagcttgcct gcatgatcgg cggcaacgtc
     1741 aacatgaact ctggcggcgc ccactgcctg cgatacggcg tcaccgccaa caacctgctc
     1801 ggcctgaaga tcgtcaccat cgagggcgac atcatcgaca tcggcggcgc ccatctcgac
     1861 gccgccggct acgactggct gggtctgttg accggcagcg aagggatgct ggcgataata
     1921 accgaggtca cggtccgcat cctgcgcagc ccggaaggtg cgagggcgat gctggcggcc
     1981 ttcaaatcga acgaaatcgc cggcgcctgc gtcgattcga tcatctcctc cggcatcatc
     2041 cccgtcgcgt tggagttcat ggatcgtccc gcgatccatg cctgcgaagc cttcgcccac
     2101 gccggctatc cgctcgacgc cgaagccatg ctcatcatcg aggttgaagg cagcgtcgag
     2161 gaacaagacc gcctgctcgg ccaactctcc gctatctgtg agaaattcga cccgatcagc
     2221 ctgcgggtct cccagtccga ggctgaatcg ctggcgatct ggaaaggccg caaaggcgcg
     2281 ttcggcgcgg tcgggcggat cagccccgac tatttgtgca tggacggcac cattcccacg
     2341 agccagctgc cgctcgtgct tcgccgcatc caggagatgt ccgacagcta cggcctgaag
     2401 gtcgccaaca ttttccatgc tggcgacggc aacctgcacc ccttgatcat gttcgacgcc
     2461 aacaatccgg ccagcttcca cgcagcggag gaattcgggg ccgacatcct gaaactctgc
     2521 gtcgaggtcg gcggctgcct gaccggggag catggcgtcg gcatcgagaa gcgcgacctg
     2581 atgccggtgc agttccatgc ccatgaactg cagcagcagc gcgatctgaa atccgccttc
     2641 gacccggact ggctgctcaa tcccagcaag gttttcccgc tgctcgaacc taaagccggc
     2701 gcgttgccgc atgccgcatg acctccgagg acgcgatcgt cgccacggtc gccgaagccg
     2761 tccgtagccg cgagcctctg ctggtccagg gcaacggcac caaggccggc atgttgcgcc
     2821 cggtccaggc agcacgaacg ctttcggcgt ccggcctgtc cgggatcaat ctgtatgctc
     2881 cgaaggaact gatcctttct gcctgggctg gcacgccgct ggtggaaatt gaagccgcat
     2941 tggccgaggg cggccagcac ctgatcgccg aacctccgga tctgtctgga ctattgggtg
     3001 ccaccgggaa gccccagacg ttcggcgggg tcgtcgccac caacctgtcc ggcccgcgcc
     3061 gcgtcgcctg gggtgccatg cgcgaccacg tcatgggaat acgagcggtg accggacgcg
     3121 cggaggtcat ccgatccggc gggcgggtgt tgaagaatgt gaccggcctc gatctgtgca
     3181 aactgctgac tggcagccac ggcacattgg gcgtgatcac cgagattacg gtgaaagtcc
     3241 tgccggcgcc ggaagccacc ggcacgctgg tgctgccggg actgacggct gccgccggtg
     3301 tcgcggctat gtcggccgct ctcggctccc cgttcggcgt ctccggtgcg acctggctgc
     3361 cggccgaggc ggttgcccgc gtgcctgggc tggccgctgt cagcggaacg gccacgctga
     3421 ttcgtatcga ggagttcgcg ccctcggtga catatcgcat cggccgcctg aacgaccagt
     3481 tcggcgtatc tggcaccatt acgctcgaca ccgcggcatc acgcgccgtt tggaaggatg
     3541 tccgcgacgc caagccactg gccgcacgcg cggaagatgc tgtttggcgc gtttccgtcc
     3601 ggccgtcggc tggtccctcc gtgctggccg cattgcagcc ggccggtgtg tcgggttttc
     3661 ttgactgggg cggcggcctg gtgtggctgg ctggccctgc ggataccgcg acccacgacg
     3721 cggtcgaggc gtcggcgaaa gcggcgaggg gcacctggac gctgttgcgc gcgcccgata
     3781 ccttgcgcgg cgcggtgcgc gtagttccgg aagaaaccgc tccgctggcg cggatcaccc
     3841 gccaggtgaa ggcggcgatg gaccccgccg ggattctcaa ccccggccgc ctctacgctg
     3901 gcctatgagg atgtttttca ttcccggctg gatcagcagt gtactgtttc tgatcacctg
     3961 ctatctcgcg ggatatcagc atgtgcagtt gcggcggccg aacccgatcc tcaatttgca
     4021 gttcctgatg atcgtctttt cgttgctcat gatcctcgtc gggacggttt acaaccgcac
     4081 ggaccagtgg ctgtccctgg cgttttttct gatcgcggcc ggtggcctga tcatcatgat
     4141 ccggcagcac cgcatgttgc cgcccaacaa gaacttcgag taacccatgc aaaccaactt
     4201 caccaccgaa cagctgcgcg acccggacac c
//
LOCUS       DASWQN010000886         7214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2459319, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000886 DASWQN010000000
VERSION     DASWQN010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2459319"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1538)
                     /locus_tag="VGC82_15025"
     CDS             complement(153..1538)
                     /locus_tag="VGC82_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424629.1"
                     /translation="MFEVLDQRTSLTGNQKRIVAAAVLGDMLEFFDYFLIGFVLAFVV
                     GPWKLTFMQSAIVLLSSGIGAMLGAAFWGWMADKIGRRPVFNLTILNFSLATGALYFT
                     PDGGWIFLSLLRTVVGFGVGGLYCVDLPLVQEFMPASKRGFIGGLVTAFIPVGVMIGS
                     LFAAYLAPAVGWRGLFAAGVLPALAILVVRVWVPESPRWLLRQGRVADAKKSLAWALE
                     MDPKDLPDPTPLESETTASNFLDLFKYPRSLIVSWLGNLGAQTGSYGVALWAPTLFML
                     ILHISPSDAAKSMIALTIGGVVGRVIFSMLSESIGRRRAGGLLGFGAALFIALAGVFH
                     SEMVFGVSVFWLLLICASFFSDGGFAVVGPYAAEVWPSHLRTSGMGSAYGFGGLGKII
                     GPLGLALVVGSSNVVKPEATLDAIQPAFLYLASWFALAGAVYFFLGIETKGRSIEEID
                     SDLKGVPVVAE"
     gene            complement(1679..2416)
                     /locus_tag="VGC82_15030"
     CDS             complement(1679..2416)
                     /locus_tag="VGC82_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HEY0424630.1"
                     /translation="MGSERNMAPQMASEPGSGSLAWQARCLLRAARSGCLATSSAGHP
                     FASLVTPACMPDGSLLLLLSQLSEHTRHLAADARCSVMVTGAAADANPQTTPRVTVTG
                     VAEVVNEPALKARFLAVHPYAALYADFSDFATWRVVPAAGLLVAGFGRASRLRAADLA
                     PEAKSVAAILASEAGILRHCNRDHPDALATIAGQPGDWRMVTADVEGFDLALGERVVR
                     FAWSAPVSDAGDVRRELVRLAEGARNG"
     gene            2484..3185
                     /locus_tag="VGC82_15035"
     CDS             2484..3185
                     /locus_tag="VGC82_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0424631.1"
                     /translation="MKQTIALVDDDRNILTSVSMTLEQEGFTVRSYTDGESALQGLTA
                     RPVDLAVLDIKMPRMDGMELLQRLRQRSSMPVIFLTSKDEEVDELMGLRLGADDYITK
                     PFSQRLLLERIRALLRRNEAGKAEGSGSAPGGVMTRGDLVLDETKHQCIWRGHDIQLT
                     VTEFLLVKALAARPGMVKSRDQLIDAAYGENIYVDDRTIDSHVKRIRKKFRIGTEEFN
                     HIETLYGIGYRYKEQ"
     gene            3199..4884
                     /locus_tag="VGC82_15040"
     CDS             3199..4884
                     /locus_tag="VGC82_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HEY0424632.1"
                     /translation="MPDGGLLSGLSRPKAPARRFRQRWVSPLLRRILLVNALPLALLV
                     VALLYLDQYQNGLLEAQVTALREQARVFAGALGESAVRTTDPDNAKLVSEVAASLLRR
                     LTEPTPDAQARLYAPDGTMLANSRMREGPGGMIAAEPLPPPLDHGPVLRTIGWIYDRV
                     LAWLPHSAPTTVVDLTPSAGGTDWQPDVKEELRLNSAGNSNETAPYIRRTRDNRLLVT
                     VAVPVERDKHTVGIISLTREAREVDESLFSVRMSILALFGLALLLTVLLSWYLSLTIA
                     RPILSLAEAAADMREGSGRSGSVPPALLRRRDEIGALASALAASSAALWERMDATERF
                     AADVAHEIKNPLSSIRSAIETLRRIEDPGRQRQLLAIIGQDVMRLDRLISDVSDASRL
                     DAELSRVTTQPVDVVPILRTLQELDEATRDEDHDPKLEVVAPATGLSVWAVEDRLVQV
                     LRNLIGNAHSFSPPCGRIVVRVRDAGGVAELSVEDAGPGIPDANLEHIFDRFYSERPS
                     GESFGQHSGLGLSISRQIVEALHGQISAENRRDGNGRVLGARFIVRLPLVVRS"
     gene            5134..5583
                     /locus_tag="VGC82_15045"
     CDS             5134..5583
                     /locus_tag="VGC82_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424633.1"
                     /translation="MPFDGLGYVATTTTAKPSIWTRLSHTMFGAPPVTKSIPLHAFQP
                     VPASQPDAVTLQVLTIGRALIEDRKDWVQRRYETRDGRRCAVGALRGAARLMNLHGPQ
                     TGANTILLSVAMSRGFNDIESMNDNSSHRQVIAAFDEAITRARHYPH"
     gene            complement(5815..>7214)
                     /gene="zwf"
                     /locus_tag="VGC82_15050"
     CDS             complement(5815..>7214)
                     /gene="zwf"
                     /locus_tag="VGC82_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY0424634.1"
                     /translation="IGVDLADLDADKWRDSLHKMMETFVGQAASESRIDKIDEDTWKR
                     LTGHISYIRGDLNDKGLYERLGQHLDQEAHGDGNCLFYLAIGDRFFAPVVTHLGQAGL
                     FDEKKDGDGKPSRWRRVVIEKPFGHDQASARALNATILRCAHEDQIYRIDHFLGKETV
                     QNIMALRFANGMFEPLWNRDRIDHVQITVAETVGVESRGSFYEKTGALRDMVPNHVFQ
                     LLAMVAMEPPGGFDAHAVRSRKSDVFSALRTVAPEDAVRGQYGPGPNGVVGYRQEKNV
                     APDSPVETYVAMELAIDNWRWAGVPFYLRTGKHMSRRVTEIAIRFKPAPYAPFEDTNV
                     AALPPNWLLLQIQPDEGIALQFEVKRPGPVVDLAAVRMAFKYADWFPKEPNIGYETLI
                     FDVMTGDQTLFNRADMVEETWRVVGEVLEAWSKDKPDGFPNYAAGSDGPADADALLKR
                     HGRAWRPTMDTATPK"
BASE COUNT         1416 a         2389 c         2193 g         1216 t
ORIGIN      
        1 catggtcctg accttcgcgg cggctttcat cttcctgttc atcgccccgg agaccgcacg
       61 caagcctttg gaagacatcg tgcgatgaag ataccggggc cgatgaaata ccggggccga
      121 tgaagatacc agttcgtccg ccgaagataa ggtcactccg cgacgaccgg cacgcccttc
      181 agatcgctgt cgatctcctc gatggaccgg cccttggttt cgattcccag gaagaagtaa
      241 accgcacccg ccaaggcgaa ccagcttgcc agatacagga aagcgggctg aatggcgtcc
      301 aacgtcgcct ccggcttcac gacgttggaa ctgccgacca ccaatgccag gccgagcgga
      361 ccgatgatct tgccgaggcc gccaaagcca taggcgctcc ccatgccgga cgtccgcaag
      421 tgcgatggcc agacttcggc ggcataaggc ccaaccacgg cgaagccacc atccgagaag
      481 aacgaagcgc agattagcag cagccagaat accgagactc cgaagaccat ctcgctatga
      541 aaaacccctg ccagcgcgat gaaaagcgcc gctccaaaac cgagcaagcc acccgccctg
      601 cgccggccga tgctttcgga cagcatcgag aagatcacgc ggccgacgac gccgccgatc
      661 gtcagggcga tcatcgactt ggctgcgtcc gacggcgaaa tgtgcagaat cagcatgaac
      721 agcgtcggcg cccagagcgc cacgccgtag gagcctgtct gagcgcccag gttccccagc
      781 caggaaacga tcaggctgcg tggatacttg aacaaatcga ggaagttgct ggcggtggtc
      841 tcgctttcca gcggcgtcgg gtccggcagg tccttcgggt ccatctccag cgcccaggcg
      901 agcgacttct tcgcgtccgc cacccggccc tggcgcagca gccagcgcgg cgattccggt
      961 acccacacgc gaaccaccag aatcgccagt gccggcagaa cgccggccgc gaacaggccg
     1021 cgccagccga ccgccggggc caggtaggcg gcaaacaggc tgccgatcat gacgccgacc
     1081 gggatgaagg cggttaccag gccaccgatg aagccccgct tgctggcggg catgaactcc
     1141 tgcaccagcg gcaaatcgac gcagtacaga ccgccaacgc caaagccgac cacggtgcgc
     1201 agcaggctca ggaagatcca gccgccgtcg ggcgtgaaat acagcgcgcc ggtggcgagg
     1261 gaaaaattga ggatggtcag gttgaacacc ggccggcggc caatcttgtc cgccatccag
     1321 ccccagaacg ccgcaccgag catcgcgccg atccccgacg ataacagcac gatcgccgac
     1381 tgcatgaagg tcaacttcca gggaccgacg acgaaggcca acacgaagcc gatcaggaaa
     1441 taatcgaaga attccagcat gtcgccgagt accgccgcgg cgacaatacg tttctggttg
     1501 ccggtcagcg acgtccgctg atcgaggact tcgaacatgc atttcctccc ccgccgggct
     1561 aagcctgctg ctcgcgccgc cgcggaaacg gtgtcacagg tcgtgcggct ctaccagagg
     1621 ctggccggcc tttggactcc agcgcagctt ccacggcgag gccgtccgcg ggcggtgatc
     1681 agccgttccg cgcgccctcc gccaaacgca ccaactcccg ccgcacatcc cccgcatcac
     1741 ttaccggcgc tgaccaggca aaccgaacca cccgttcgcc caacgccagg tcgaaccctt
     1801 ccacatccgc cgtcaccatc cgccaatcgc ccggctgtcc ggcgatcgtt gccagtgcat
     1861 cgggatgatc gcggttgcag tgcctcagaa ttcccgcttc ggaagccaag atagccgcga
     1921 cggattttgc ctccggcgcc aggtccgccg cccgaagccg cgacgctcgg ccaaacccgg
     1981 ccaccagcaa tcccgccgcc ggcacaaccc gccacgtcgc gaaatcgcta aaatcggcat
     2041 acagtgccgc ataaggatgc accgccagaa accgggcctt cagcgccggc tcgttcacca
     2101 cctcggccac gccggtgacg gtcacccgcg gcgtggtttg cggattcgca tccgccgccg
     2161 cgccggtcac catcaccgaa caccgcgcat cggcggccag atgtcgcgta tgctccgaaa
     2221 gctgcgacag cagcagcagc agcgagccat ccggcatgca agccggcgtt accagtgacg
     2281 caaacgggtg gccggcggaa cttgtcgcca gacaaccgct tcgcgctgcc cgcagcaaac
     2341 acctggcttg ccaggcgaga cttccggatc ctggttccga cgccatctga ggtgccatat
     2401 tccgttcact tcccatccgt aatccgccac aatcatccac catatcatgc caggatggta
     2461 aacggacctt caaggagacc acgatgaagc agaccattgc cctggttgac gatgaccgca
     2521 acattctgac cagcgtctcc atgaccttgg agcaggaagg cttcacggtc cgcagctaca
     2581 ccgacggcga aagcgcgctg cagggcctga ccgcccggcc cgtggacctc gcggtgctag
     2641 acatcaagat gccccgcatg gatggaatgg aactgctgca gcgcctgcgc cagcgcagct
     2701 cgatgccggt gatcttcctg acatcgaagg atgaggaagt cgatgaactg atgggccttc
     2761 ggctcggcgc cgacgattac atcacaaaac cgttcagcca gcgcctgttg ttggagcgga
     2821 tccgtgcctt gctgcgccgc aacgaagccg gcaaggccga aggctccggc agcgctccgg
     2881 gcggcgtcat gacccgcgga gacttggtgc tggacgaaac caagcaccaa tgcatctggc
     2941 gtggccacga catccagttg acggtgaccg agttcctgct ggttaaagcg ctggccgccc
     3001 ggcccggcat ggtgaaaagc cgcgaccaac tgatcgatgc cgcctatggc gagaacatct
     3061 atgtcgatga tcgcaccatc gacagccacg tgaagcgcat tcgcaaaaag ttccgcatcg
     3121 gcacggaaga attcaaccac atcgaaacgc tctacggcat cggctaccgg tacaaagagc
     3181 agtaatcagg agcattcatt gcctgacggc gggttgttat ccggcctgtc ccggccgaag
     3241 gcgccggcga ggcgcttccg ccagcgctgg gtgtcgccgt tgctgcggcg catcctgctg
     3301 gtcaacgcgc tgccgctggc gctgcttgtc gtggcgctgc tgtacctgga ccagtaccag
     3361 aacgggctgc tggaagcgca ggtcaccgcg ctgcgagagc aagcccgcgt gtttgccggc
     3421 gcactgggcg aaagtgccgt gcgcacgacc gatcccgata atgcaaagct ggtttcggaa
     3481 gtcgcggcct cattgctgcg ccgcctgacg gaacctacgc cagacgccca ggccaggtta
     3541 tacgcccccg acggaaccat gctggcgaat tcccgaatgc gggaaggacc tggcggcatg
     3601 atcgccgccg aaccactgcc gccgccgctc gatcatggac cggtattgcg aacgatcggc
     3661 tggatctatg accgcgtgct ggcgtggttg ccgcacagcg ccccgaccac ggtggtcgac
     3721 ctgacgccct cggccggcgg aaccgactgg cagcccgacg tgaaggaaga attgcgcctc
     3781 aacagcgccg gcaatagcaa tgaaaccgcg ccgtatatcc ggcgcacccg cgacaaccgc
     3841 ctgctcgtca ccgtcgctgt gccagtggag cgggacaagc ataccgtcgg aatcatctcc
     3901 ctgacgcggg aggcgcggga ggtggacgag agtctgttca gcgtgcggat gtcgatcctg
     3961 gcgctgttcg gcctggcgtt gctgctgacc gtgctgttgt cgtggtatct gtccctgacc
     4021 atcgcacgcc caatcctgag cctggcagag gccgccgcgg acatgcgcga aggcagtggg
     4081 cgatccggca gcgtgccgcc ggccctgctg cggcggcggg atgaaatcgg cgcgctggcc
     4141 tccgcactcg cggcctcatc cgctgcatta tgggagcgaa tggacgcgac ggaacgcttc
     4201 gccgccgacg tggcgcatga gatcaagaac cccctctcgt cgatccgcag cgcgatcgaa
     4261 accctgcgcc gaattgaaga tcccggtcgc cagcggcaac ttctagcgat catcgggcag
     4321 gacgtgatgc gcctggaccg gctgatcagc gatgtctccg acgcgtcgcg cctggatgcg
     4381 gaactgtcgc gggtcacgac acagccggtc gacgtcgtgc cgatcctgcg caccttgcag
     4441 gaactcgacg aggcgacgcg tgacgaagac cacgatccaa agctggaggt cgtcgctccg
     4501 gccaccgggc tgtccgtttg ggcggtagag gaccgcctgg ttcaggtgct gcgcaacctg
     4561 atcggcaacg cgcattcctt cagcccgcca tgcggcagga tcgtcgtgcg ggttcgcgac
     4621 gccggtggtg tcgcggagct gagcgtggaa gacgcgggcc ccggcatccc cgatgccaac
     4681 ctggagcaca tcttcgatcg gttctactcg gaacgtccca gcggcgaaag cttcggacag
     4741 cactccggcc tggggctgtc gatcagccgg cagatcgtcg aggcgctgca tgggcagatc
     4801 tccgccgaga atcgccgcga tggcaacggc cgcgtgctgg gcgcccgctt catcgtccga
     4861 ctgccgctgg tggtccgatc ctgacgcttt ggtgcccgag cggcaggcgg atcgcctgca
     4921 cctgcgctgg tatcagcaag ccgacggacg accatcgcag gacttctgag ccggtttgga
     4981 gcggccgcaa gcttaaactg gcattgagaa accgtttgtg atcggtaaag gcttggcatc
     5041 atcgcaatcg tcagccaact tatcgtgcac ggtgaacaac cgaggcgaaa acgtggttta
     5101 acgcttggca ccctgcaact atgggaggca cccatgccgt ttgatggact tggttacgta
     5161 gctaccacca ccaccgcgaa gccttcgatc tggacgcgcc tgtcccacac gatgttcggg
     5221 gcgcctcccg ttacgaagtc aatcccgctc catgcgtttc aaccggttcc ggccagccag
     5281 ccggatgcgg tgacgctgca ggttctgacg atcggccgag cgctgatcga ggaccggaag
     5341 gattgggtac agcggcgtta tgaaactcgc gacgggagac gctgtgcggt tggtgccctt
     5401 cgtggcgccg caaggctaat gaacctgcac ggcccacaga ccggggcgaa cacgattctg
     5461 ctctcggttg ccatgagccg cggctttaac gacatcgaga gcatgaacga caactccagt
     5521 caccgccagg tgattgcggc gttcgacgag gctatcacgc gggctcggca ctaccctcac
     5581 tagagggcgt ctggtgtcat ggccgtgctt ggcgcggcca tccgcgaatt tgtccgccca
     5641 tggcaaagtc gtggatggcc gttacaaggc tgggcatgac acgggcggta aaggcccctg
     5701 aggtagcgac tattgcggcc tgcatgggct agatttggca cgacgtgacg ggccgggatg
     5761 acccgcctcg atccggtatt ccaacaaaga ttcaccatgc cgcgctcgcc ccgatcactt
     5821 gggcgtagcg gtatccatcg tcggccgcca tgcgcggccg tgccgcttca gcagtgcatc
     5881 cgcatcggcg ggtccgtcgc tgccagcggc atagttcgga aagccgtcgg gcttgtcctt
     5941 tgaccaagct tccagaacct caccaacaac ccgccaggtt tcctcgacca tgtccgctcg
     6001 gttgaagagt gtctggtcac ctgtcatgac atcgaagatc agcgtttcgt aaccgatgtt
     6061 cggttccttg gggaaccagt cggcgtactt gaacgccatg cgcaccgcgg ccaaatccac
     6121 caccgggccg ggacgtttca cctcaaactg cagcgcaatc ccttcatccg gttggatctg
     6181 cagcagtagc cagtttggcg gcaacgcggc gacgttggta tcctcgaagg gcgcatacgg
     6241 cgccggcttg aagcgaatag cgatttcggt gacccggcgg gacatatgct tgccggtacg
     6301 caagtaaaac ggcacccccg cccagcgcca attatcgatc gcaagttcca tcgcgacata
     6361 ggtttccacc ggcgagtctg gcgcaacgtt cttctcctgg cggtatccga ccacgccgtt
     6421 gggaccgggg ccgtactggc cacgcaccgc gtcctcgggt gctacggtac gcagggcaga
     6481 aaacacgtcc gatttacggc tgcgcacggc atgggcgtca aagccgccag gaggttccat
     6541 cgccaccatt gccagaagct gaaacacatg attcggcacc atgtcgcgca acgcgccggt
     6601 tttctcgtag aagctacctc gggattccac gccgaccgtt tccgcgacgg tgatctgcac
     6661 atggtcgatg cggtcgcggt tccacagcgg ctcaaacatc ccgttggcga agcgcagcgc
     6721 catgatattc tgcaccgtct ccttcccgag gaagtggtcg atccgataga tctggtcctc
     6781 gtgcgcgcag cgcaagatgg tggcgttgag cgctctcgcc gaagcctgat cgtggccgaa
     6841 cggcttttcg atgaccacgc ggcgccagcg actgggtttg ccgtccccgt ccttcttttc
     6901 gtcgaacagt cccgcctggc ccagatgagt gaccacaggc gcgaagaagc ggtccccgat
     6961 ggccaggtaa aacaggcagt tgccgtcgcc gtgcgcctcc tggtccaggt gctggccgag
     7021 cctctcatag agccccttgt cattaaggtc gccgcggatg taggaaatat ggccggtcag
     7081 ccgcttccag gtgtcctcgt cgatcttgtc gatccggctt tcgctcgcag cctggccgac
     7141 gaaggtctcc atcatcttgt gcaggctgtc gcgccacttg tcggcatcca ggtcggcaag
     7201 atcgaccccg atca
//
LOCUS       DASWQN010000887         4086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2327618, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000887 DASWQN010000000
VERSION     DASWQN010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2327618"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VGC82_15055"
     CDS             <1..1055
                     /locus_tag="VGC82_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY0424635.1"
                     /translation="IEPVIAGVFEEFAGGRPVTKPPFPRIPYDQAMLEFGSDKPDLRN
                     PIRIADVTEHYAGSGFGLFAGIAASGGIIRAIPAPGAGAQPRSFFDKLNDWAKAEGQG
                     GLGYITFDADGPKGPIARNLEAGRAEAIRVACGLQAGDAVFFVGGKRDAAPKFAGTVR
                     TRLCEELGLLEKGTFRFCWVTDFPMYELNEETGLVDFSHNPFSMPQGELHALNTQDPL
                     TIKAFQYDIVCNGIELSSGAIRNHRPDIMIRAFEIAGYTAADVEARFGGMLNAFRYGA
                     PPHGGSAPGIDRMVMLLADEPNIREVILFPLNQQGEDLMMGAPAEIPPARMKELSLKL
                     DLPPVRPKAAPPVTGG"
     gene            1144..1968
                     /locus_tag="VGC82_15060"
     CDS             1144..1968
                     /locus_tag="VGC82_15060"
                     /inference="COORDINATES: protein motif:HMM:NF020478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HEY0424636.1"
                     /translation="MRLPAALGALFVTAAIVAQPTAAASAPAPTAIPAQPGLLAHKAL
                     YTLTLGQAKGNDVIAARGTMGYEVTDACDGWAVRQRLRMTITNAEGQEIEMASDYATW
                     ESKDGLKFRYHMRQTTDTAVTSQTDGEALLPKQGSAGEAHYTTPHDATSPLPAGTVFP
                     MAHTAAIIAGARDKKHFVSLPLFDGTDENGLEDSFIVVLDWKPPMQTKWPALSPLSST
                     RVSLSFFDHAPAAITPSYEVGMRYWENGVADDMKMNFGDFVMNAKMKEFAPQPRRC"
     gene            complement(1994..2212)
                     /locus_tag="VGC82_15065"
     CDS             complement(1994..2212)
                     /locus_tag="VGC82_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424637.1"
                     /translation="MSDEAGGGQSHDKTLEDTFPASDPPANSGITGAETTDKPSNERN
                     IDEIPTGRPTSDRHATETAHQWENEEKS"
     gene            2317..3039
                     /locus_tag="VGC82_15070"
     CDS             2317..3039
                     /locus_tag="VGC82_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY0424638.1"
                     /translation="MTTPIRLGMLTPSSNTTLEPVTAAMVAALPAVSVHFSRFRVTEI
                     GLTDDALAQFDDRPILAAAELLAHAKVDVIAWNGTSAGWLGFDADRRLCERIEAATGI
                     RASTSMLALNEILAATGVKRFGLVTPYIDAVQQRIVANYAAIGITCDTERHLGIQDNF
                     AFAEVPHSVLTGMIRDVAAQRPDAIGVICTNLKAAPLVAALEQETGIPIYDTIATVVW
                     KSLKLAGVDPSGVTGWGRVFKI"
     gene            complement(3184..>4086)
                     /locus_tag="VGC82_15075"
     CDS             complement(3184..>4086)
                     /locus_tag="VGC82_15075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424639.1"
                     /translation="PNAAVLIGARILQSLGGCAGLVLGRAAVRDGATVDKAASQLALL
                     TLVMAMVPAVAPAIGGYLTAYIHWRASYVLLALFGASTLLACVLLMPETLSLQASGRR
                     SMSVAYVRLLRSPRFLGYAVGGACTTTAFYGFMSASPFIFETHLHQPAQRVGLYYLLL
                     MLGVAAGSFGANRIAGRLPVRKALRIANGLGILGAALFALADAADVLSVATVVGPVCL
                     FMMGAGMASPFALAGSVSVNPQAIGAASGLYGFSQMGYGMLCTVVVETWHPGAVYPVA
                     AVLLGSALLGQAAMAIAVWAEPRR"
BASE COUNT          732 a         1360 c         1302 g          692 t
ORIGIN      
        1 ccatcgagcc ggtcatcgcc ggtgtcttcg aagaattcgc cggcggccgg ccggtgacca
       61 agccgccgtt cccgcgcatc ccctacgatc aggcgatgct ggagttcgga tcggacaagc
      121 cggacctgcg caacccgatc cgcatcgccg acgtgaccga acactatgcc ggttccggct
      181 tcggcctgtt cgctgggatt gcggcgtccg gcggcatcat ccgcgccatc ccggcacccg
      241 gcgccggcgc gcagcctcgc agcttcttcg acaagctgaa cgattgggcg aaggcggaag
      301 gccagggcgg ccttggctac atcacatttg acgcggacgg tccgaaaggt ccgatcgccc
      361 gcaatttgga agccggccgc gccgaggcga tccgcgttgc ctgtggactg caagccggcg
      421 acgcggtgtt cttcgtcggc ggcaagcggg atgccgcgcc gaaatttgcc ggcacggtcc
      481 gcacgcgcct gtgcgaggaa ctcggcctgc tggagaaggg cacgttccgg ttctgctggg
      541 tcaccgattt tccgatgtac gagctgaacg aggaaaccgg cctggtcgat ttcagccata
      601 acccgttcag catgccgcaa ggcgagcttc acgcgttgaa cacccaggac ccgctgacca
      661 tcaaggcctt ccagtacgac atcgtctgca acgggatcga gctgtcgtcg ggcgccatcc
      721 ggaatcaccg cccggacatc atgatccgag cgttcgagat cgcggggtac acggcggcgg
      781 acgtggaggc gcgcttcggc gggatgctga acgcgttccg ctacggcgcc ccgccgcatg
      841 gcggatcggc acccggcatc gaccgcatgg tgatgctgct ggccgacgag cccaacatcc
      901 gcgaagtcat cctgttcccg ctgaaccaac agggcgagga cctgatgatg ggcgccccgg
      961 ctgaaattcc gccggctcgg atgaaggaat tgtcgctgaa actcgatctg cctccggtaa
     1021 ggcccaaggc ggcgcctccg gtcacgggtg gctgaggcaa cggcgaaacc ggttgctggg
     1081 cggtggcgcg atgccgcccg gtatgcctat gctctgcaac gacaattttt cgcggagtaa
     1141 ttcatgcgcc ttcccgccgc tcttggcgcg ctgtttgtca ctgccgcgat cgtggcccag
     1201 ccgaccgccg cggccagtgc cccggcgccg acagcgatcc ccgctcagcc gggcttgctt
     1261 gcccacaagg cactctacac gctgaccctc ggccaggcga agggcaacga cgtgatcgcc
     1321 gcgcgcggta cgatgggtta cgaggtgacc gatgcctgcg acggctgggc ggtgcgccag
     1381 cggctgcgca tgaccatcac caatgccgaa ggccaggaga tcgaaatggc ctcggactat
     1441 gccacctggg agtccaagga cgggctgaag ttccgctatc acatgcggca aaccaccgac
     1501 acggcggtca ccagccagac cgatggcgag gccctgttgc cgaaacaggg cagcgccggg
     1561 gaggcgcact acaccacacc gcatgacgct acgagcccgc tgcccgccgg gaccgtattc
     1621 ccgatggctc ataccgccgc gatcatcgca ggggcgcgcg acaagaagca ttttgtcagc
     1681 ctgccgctgt tcgacggcac cgatgagaac ggcctggagg acagcttcat cgtcgtgctg
     1741 gactggaaac cgccgatgca gaccaagtgg ccggctctgt cgccgctttc cagcacacgc
     1801 gtcagcctga gttttttcga ccacgcccca gccgcgatca cgccgagcta tgaagtcggc
     1861 atgcgctact gggaaaacgg cgtggccgac gacatgaaga tgaatttcgg cgatttcgtc
     1921 atgaacgcga agatgaagga atttgccccg cagccgcgcc gctgttgagg gccgggagca
     1981 aacccattcg gcttcaggat ttttcttcat tctcccactg gtgtgccgtc tccgtcgcat
     2041 gccggtcgga ggtgggccgt ccagtcggta tctcgtcgat gttgcgttcg ttcgatggtt
     2101 tgtccgtggt ttcggcaccg gtgatgcctg aatttgccgg cgggtcgctg gccgggaacg
     2161 tgtcttccag tgtcttgtcg tggctttgtc cgccgcctgc ctcgtctgac atgtcgtttc
     2221 ctttcctccg gcggaaatgc cggtcgtgac ttgcaacgcc gcagatcggc cgcggttcgg
     2281 cttcacgccc cacgccgccc gtgcgaaact tccgccatga ccacacccat tcggctcggt
     2341 atgctgaccc cgtcctccaa cacgacgctg gagccggtga ccgcggcgat ggtggctgct
     2401 ctacctgcgg tttcggtgca tttctcccgc ttccgcgtga ccgagatcgg cctgaccgat
     2461 gatgcgctgg cgcagttcga cgatcgtccg atcctggcgg cagcggaact gctggcgcac
     2521 gccaaggtcg atgtgatcgc ctggaacggc acctccgccg gctggcttgg attcgacgcg
     2581 gaccggcgcc tgtgcgagcg tatcgaggcc gccaccggta tccgtgccag cacgtcgatg
     2641 ctggcgttga acgaaattct cgccgcgacg ggggtgaagc ggttcgggct ggtgacgccg
     2701 tacatcgatg cggtccagca gcggatcgtc gccaactatg ccgcgatcgg catcacctgc
     2761 gataccgaac ggcatcttgg catccaggac aatttcgcgt tcgccgaggt cccgcattcg
     2821 gtgctgacgg ggatgatccg tgacgtcgcg gcccagcgtc cggatgcgat cggggtcatt
     2881 tgcaccaacc tgaaggccgc accgctggta gcggcgctgg agcaggagac cggcatcccg
     2941 atctacgaca cgattgccac cgtggtatgg aagagcctga agctcgcggg ggtcgatcct
     3001 tcgggcgtga ccggctgggg gcgggtgttc aagatttaga acgtaatcgc gttgggcgga
     3061 acgtttgaag cgtgtgaatc acgcgataag ataaggaggt ggatcatttg cggcctgcac
     3121 agtcaggctg aaaatggtcc aacctgcgcc gataaacgcc tccctgggat gtcctgcggt
     3181 tttctatcgc cgcggttcgg cccacaccgc gatcgccatt gcggcctgcc cgagcagcgc
     3241 cgaccctagc aggacggcgg cgaccggata caccgcgccg ggatgccagg tttccaccac
     3301 gacggtgcaa agcatcccgt agcccatctg gctgaacccg tacaagccgg acgcggcccc
     3361 gatcgcctgt gggttgacgc tgaccgatcc ggccagggcg aacgggctgg ccatgcccgc
     3421 ccccatcatg aacaggcaaa ccggcccgac caccgttgcc accgacagga catcggctgc
     3481 atcggccagc gcaaacagcg cggcaccgag tatccccagc ccgttggcga tccgcagcgc
     3541 ctttcgcacc ggaaggcgtc cggcgatgcg gttggccccg aaactgcccg ccgcgacgcc
     3601 cagcatcagc aacaggtaat agagtccaac cctttgcgcc ggctggtgca gatgcgtctc
     3661 gaagatgaac ggcgaggccg acatgaaccc atagaacgcc gtggtcgtgc atgctccgcc
     3721 gaccgcatag ccgaggaatc gcggcgaccg cagcagccgg acataggcaa cgctcatgga
     3781 ccggcgaccg ctcgcctgca gggacagcgt ttccggcatc agcagcacgc aagccagcag
     3841 ggtcgaagcg ccgaacagcg ccagcagcac gtagctggcc cgccaatgga tataggccgt
     3901 aaggtagcca ccgatcgccg gcgcgaccgc cggcaccatc gccatcacca aggtcagcaa
     3961 cgcaagctgc gaggccgctt tatcgaccgt ggcgccgtcg cgcaccgccg cccgtcccag
     4021 aaccaacccg gcacagccac ccagcgactg caggatcctc gccccgatca gcacggctgc
     4081 gttcgg
//
LOCUS       DASWQN010000888         1631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_349590, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000888 DASWQN010000000
VERSION     DASWQN010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349590"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VGC82_15080"
     CDS             <1..275
                     /locus_tag="VGC82_15080"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0424640.1"
                     /translation="LESRCCRWLLMMQDRIGDDDLPLTQECLATMLGSGRPRINGLLA
                     TLEQNGLVRRYRGRIRLLSRPGLERRACECYRLLRHAPPVRGTEPF"
     gene            445..810
                     /locus_tag="VGC82_15085"
     CDS             445..810
                     /locus_tag="VGC82_15085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0424641.1"
                     /translation="MANDSACRPRLLLVEDDRLVRETIVLMLEDDYEVVHDASVGDAL
                     IRLGAPGTPPIDVLLLDCLLPDGNVSVVLAVADQRSIPVVLISGDASMIERLDPGRLF
                     LPKPFSQASLLTVLDSARG"
     gene            838..>1631
                     /locus_tag="VGC82_15090"
     CDS             838..>1631
                     /locus_tag="VGC82_15090"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEY0424642.1"
                     /translation="MDIHRINLHLVSDATGETLNAIARATTVQFEPAQIIHHRWSLIR
                     TRFQLHRVLEGIEAEPGPVLSTLIDRALRLELDTTCQRLNVKVVNVLDPVIEALQDTI
                     GQKAEARPGRQYVLNADYFSRIDAMHYVLAHDDGQAQPGLAGADVVLVGVSRSSKTPT
                     CFYLANRGIKAANVPLVPNLPIPPGLEDIKCPVIGLSLAPEALIDIRRHRLRLIGGGG
                     QTGPIRADSSEYVDEDAVKAELLWARRFCNSHGWPTIDVTRRSIEET"
BASE COUNT          293 a          509 c          515 g          314 t
ORIGIN      
        1 gcctcgaatc ccggtgctgc cggtggctgc tcatgatgca ggacagaatc ggcgatgacg
       61 accttccgct gacccaggag tgtctggcca ccatgctcgg gagcggccgt ccccggatca
      121 acggcttgct ggcgacgctg gaacaaaatg gcctggttcg gcgctatcgc ggccggatcc
      181 gcctgctgtc gcgcccaggg ctggagcgcc gggcctgcga gtgctaccgc ctgcttcgcc
      241 atgcgccgcc cgtgcgcggg actgaaccct tttagaccga tattccggct gccttggctc
      301 acagccatcc ggctcatttc agtcggttaa acagtccgct gccgcggatg agcatttcct
      361 gcgtgcgccc gtacattgaa ccgtcacttc ggtaacagac ggatactccg gtccggacta
      421 ctttgcctta aaaaggtaca atccatggca aacgattcgg cgtgccgccc gcgcttgcta
      481 ctggtcgagg acgaccgcct ggtacgggaa acgatcgtcc tgatgctcga ggatgattat
      541 gaagtcgtac atgacgcgtc ggtgggcgat gctttgatac gactaggagc ccccggtacg
      601 ccgccgatcg acgtattgtt gctcgactgc ctgctgccgg acggcaatgt ttcggtcgtc
      661 ttggcggtgg cagaccagcg gtctatcccg gtcgtgctga tcagcggcga tgcaagcatg
      721 atcgagaggc tggatcctgg gcggctgttc ctgccaaaac cgttcagcca agcgagcttg
      781 ctcaccgtcc tggatagtgc tcgcggctga accgcaagcc gtctatgctt gccagtcatg
      841 gacatccacc gcatcaatct gcatttggtt tcggacgcga ccggggaaac gctgaacgcc
      901 atcgcccggg caacgactgt ccaatttgag ccggcacaga tcattcatca ccgttggagc
      961 ctgatccgca cgcgtttcca gcttcaccgt gtgctggaag gtatcgaggc cgaacctggc
     1021 ccggttctgt ccaccctgat cgaccgcgcc ctgcggctgg agctggacac cacctgtcaa
     1081 aggctgaacg tcaaggtcgt caacgtcctg gatccggtta tcgaggcact ccaggacacg
     1141 atcggccaga aggcggaggc ccgacccggc cggcaatacg tgctgaacgc cgattacttc
     1201 tcgcgtattg acgcgatgca ctacgtgctg gcgcatgatg acgggcaggc acagccgggc
     1261 ctggccgggg ccgatgtggt gctggttggc gtgtcgcgca gttcgaagac gccgacctgc
     1321 ttctatctgg ccaatcgcgg catcaaggcg gcgaacgtgc cgttggtgcc aaaccttccg
     1381 atcccgccgg ggctggagga catcaagtgt ccggtgatcg gactgagcct ggcgcccgag
     1441 gcgctgatcg acatccgccg gcaccgcttg cggctgatcg gcggaggcgg ccagaccggc
     1501 ccaatccgcg ccgacagttc cgaatacgtg gacgaagacg cggtcaaggc cgagctgttg
     1561 tgggctcggc gattctgcaa cagccatgga tggccgacga tcgacgtgac tcggcgctcg
     1621 atcgaggaga c
//
LOCUS       DASWQN010000889         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2196597, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000889 DASWQN010000000
VERSION     DASWQN010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196597"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VGC82_15095"
     CDS             <1..821
                     /locus_tag="VGC82_15095"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0424643.1"
                     /translation="PYENNLAFDMIAQACGGTFSVTGDKDGPPIRPGISLGDTGTGML
                     MAITILGALYKRKETGEGHRLQVAMQDAIMHYMRINFATQGRTGKAVGRAGSKVPGIS
                     NAPMGLYPCAPGGSNDYVYIMTSRANPDHWDRLLKVVGREELIGDSRYATPHDRVQRE
                     QEVDEIIATWTRNRTKRDAMRAVGEAGIPAGAVFDTMELESDRSFEDRGIMQVMRHPV
                     HRDFKMPGWPVRVDGKPPALKSSPVLGEHTDAVLVEWLGLNAQAIAAMRAEGAV"
     gene            1017..1442
                     /locus_tag="VGC82_15100"
     CDS             1017..1442
                     /locus_tag="VGC82_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424644.1"
                     /translation="MTWTRITDPETVAGLLPAWLGRRMVGLRGSFGLLLTTGDVLRLT
                     TIAAAHQSSHGVILLDVLLDNAGPPDGLDLAWRSKHYLGAPVPGATVATVNLAHVVTA
                     VEFTVAEMAQHPGDGTVNFGDEVDEPIETRQTVAVGQSA"
BASE COUNT          294 a          450 c          522 g          242 t
ORIGIN      
        1 gcccgtacga gaacaacctc gcgttcgaca tgattgccca ggcgtgcggc ggcaccttca
       61 gcgtgaccgg cgacaaggac gggccgccga tccggccggg aatctcgctc ggcgataccg
      121 ggacggggat gctgatggcg atcaccatcc tcggcgcgct gtacaagcgg aaggagaccg
      181 gggaaggcca ccggctgcag gtcgcgatgc aggatgcgat catgcactac atgcggatca
      241 atttcgccac gcagggccgg accggcaaag cggtcggccg tgccggcagc aaggttccag
      301 gcatcagcaa tgcgccgatg ggattgtatc cgtgcgcgcc gggcggctcg aacgactacg
      361 tttacatcat gaccagccga gccaaccccg accattggga ccggctgctg aaggtcgtgg
      421 gacgcgagga gttgatcggg gattcgcgat acgcaacgcc gcacgaccgg gtgcagcggg
      481 agcaggaggt ggacgaaatc atcgccacct ggacccgcaa ccgtaccaaa cgcgatgcga
      541 tgcgggcggt gggagaagcg gggatcccag ccggtgccgt gttcgatacg atggaactgg
      601 agagcgatcg cagtttcgaa gaccggggca tcatgcaggt tatgcgccat ccggtgcatc
      661 gcgacttcaa gatgccgggc tggccggttc gcgttgacgg caagccgcca gcactgaaat
      721 cgtcgccggt gctgggcgaa cacaccgacg cggtgctggt cgagtggctt gggctgaatg
      781 ctcaggcgat cgccgcgatg cgggcggagg gagcggtcta ggctcccgct gccgccaaag
      841 cccatatcag gccggcgcgc ccgccgcgac tttttgcggt caccgcgctt tttcatagtc
      901 ctcagcagca agagcgtgat taatttggct ccggaagatg gctacggggc ctgaaggggt
      961 tttgccgccg ccgccgtgac catatcaacg aggtgaaccc cgaggaggac cgaatagtga
     1021 catggacgcg aatcacggat cccgagacgg tggcgggcct gctgccagca tggctgggac
     1081 ggcgcatggt cggactgcgc ggatcgttcg gcctgctgtt gacgacaggc gacgtcttgc
     1141 ggttgaccac gatcgccgcg gcgcatcaat cgtcgcacgg ggtgattcta ttggacgtgt
     1201 tgctggacaa cgccggccca cccgacggac tcgacctggc atggcggtcg aagcattacc
     1261 tgggggcccc ggttccgggt gcgacagtcg cgaccgtcaa tctggcgcac gttgtcacgg
     1321 cggtcgaatt taccgtcgca gagatggcgc aacaccccgg tgacggcaca gtgaacttcg
     1381 gcgacgaagt ggatgaacca atcgaaaccc ggcagaccgt ggcggttgga caatcggcat
     1441 agcggaaccc agaacgcaaa ctatcggcct ctcgtcggga tcgccgtccc gcgagaggcc
     1501 gatttgcg
//
LOCUS       DASWQN010000890         3897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2196638, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000890 DASWQN010000000
VERSION     DASWQN010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196638"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..2138
                     /locus_tag="VGC82_15105"
     CDS             246..2138
                     /locus_tag="VGC82_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEY0424645.1"
                     /translation="MDAIVKRDASVRASPADRPLTAGGALAALGIVYGDLGTSPLYTF
                     QTIAGTVGGHPSTQDALGLLSLVVWALVITVSIKYCVFVMRADNHGEGGILALMALVT
                     RRSTGALVVAGLFGAALIYGDGVITPAISVLSALEGINVLTDMFKPYVLPAALLVLLL
                     LFGAQMKGTATIGRVFGPVMLLWFAVIATLGMMGLWHRPEVLLAVDPRHAIRFIFAHG
                     LHTFVVLGGVFLAVTGGEALYADMGHIGRNPIRLSWYCIVLPALLLCYAGQTALLVEN
                     PGLEGNPFFKLAPNWAVVPLVILATCATIIASQAIITGAFSLTRQAMQLGWFPGLNIR
                     QTSDQEYGQIYVPVVNWTLMACTLFLTFSFGSSDKLAGAYGTAVSTTMLLTTALLFNA
                     MRDVWRWPVIIAVLVSGAFLLIDLTFFGANLMKIREGGWIPLLLGGLVFVVMTTWHRG
                     VEAVRRTLVQKPETAAEFLAQLNKGTITRVPGTAVFLSRSETAIPAVMVRHVAQIKAL
                     QETVVSLTVRFEDYPRVPLEKRAEVEKIAEGLWHVTIRFGFVEMPNVAAALACAKEKG
                     CQLNLDDAVYFAAHDDVVRSANPPRLAAWRRMLFALMYRNAVRAPDRFELPADKFLEV
                     GRQIAL"
     gene            2264..>3897
                     /locus_tag="VGC82_15110"
     CDS             2264..>3897
                     /locus_tag="VGC82_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424646.1"
                     /translation="MTTATLSRRMMLSAIAASPVAMDFLRGAMAEAADLTASGLVGEL
                     QGPTMIVDPAKWPKTFHEAPMLAEQVKAGKLPPVAERVPAEPMVWQPLNEVGKYGGTW
                     RRAFTGPADGENGNRIQSSDKPLHWSADGSKIVPCVAKGYALSDDGKTYTLFLRKGMK
                     WSDGTAFTADDFIFWYEDIYGNPEIVPTPTPEMRPRGKPGRMVKVDETTVRWEFDVPY
                     YLFEDIMAGDTAMGGGQAVRQAAKVSFGGYAPAHYLKQFLPKYSSVEAANARAKQEGF
                     ENWVQMLHFKKDWELNIEVPVLGPWRTVRPINTAIWLLERNPYYYAVDTAGNQLPYFD
                     RVQFTLAENLEIINLRAMAGEYDEQERHIDLGKLPVLLDNQEKGNYKVHLDLGFAGSD
                     FMFHVNQSFTGDAEIRKWLTNADFRRALSLGIDRDQLNETFWLGVGTPGSPAPGESMP
                     QSPGADWRKKWSTYDPALANKMLDGIGLAKKDNEGFRLRTDSGERLRIQVIAIAAFLP
                     YPKLSEMGADQWRKIGIQADVREMERGLGFAKQTNN"
BASE COUNT          687 a         1185 c         1283 g          742 t
ORIGIN      
        1 ccggcggcag cggcgctgta ccgaccggtc acccggcgtt tggcaagacc gccggaaccc
       61 accaggccgc ggcttatgtg tgccggcgta acgcttgcgg cctgccggtc atggatgccg
      121 ccgttctgtc gcaggcactc cgcacgcggg tttgatcgcg gggcggttcc tccgaacgcg
      181 ttcgggcgca gatggccccg cttgacccgg ccggtctggg gcgggcggct ccggcggaga
      241 agtcaatgga tgccattgtg aaacgagatg cctcggtgcg tgcctcgccg gccgatcggc
      301 cgcttaccgc cgggggggcg ttggccgcgc ttggcatcgt ttacggcgac cttggcacca
      361 gcccactcta taccttccag accatcgccg ggacggtcgg tggtcatccg tccacccagg
      421 acgcgctggg gctgttgtcg ctggtcgtat gggcgcttgt cattacggtg tcgatcaaat
      481 actgcgtttt cgtgatgcgg gcggacaacc acggcgaggg tggtatcctg gcgctgatgg
      541 cactggtgac gcgacgctcc accggtgccc tggtggtagc ggggctgttc ggcgcggcac
      601 tgatctatgg cgatggcgtc attacgccgg cgatctccgt gctgagcgcc ctggagggca
      661 tcaatgttct gaccgacatg ttcaagcctt atgtgctgcc ggccgccttg ttggttctgc
      721 tgctgttgtt tggcgcccag atgaagggca ccgccactat cggccgggtt ttcggtccgg
      781 tcatgctgct gtggttcgcg gtcatcgcga ccctgggcat gatggggttg tggcaccggc
      841 cagaggtgct gctcgcggtc gatcccaggc atgcgatccg gttcatcttc gcccacggcc
      901 tgcacacgtt cgtcgtgctc ggtggcgtat tcctggccgt caccgggggt gaggccttgt
      961 atgccgatat gggccatatc ggccgcaatc cgatccgcct gtcctggtat tgcatcgtac
     1021 tgccggcctt gttgctctgc tatgccggcc agaccgcgct gctggtggaa aaccccggtc
     1081 tggaggggaa tccgttcttc aagctggcgc cgaactgggc ggtggtgccg ctggtgatac
     1141 tggcgacctg tgccacgatc atcgccagcc aggccatcat taccggcgcg ttctccctga
     1201 cccggcaggc gatgcagctt ggctggttcc ccggcctgaa catccgccaa acctcggatc
     1261 aggaatacgg ccagatctac gtgccggtgg tgaactggac gctgatggcc tgcaccctgt
     1321 tcctgacttt cagcttcggt agctccgaca aactggccgg cgcttacggc accgccgtct
     1381 caaccacgat gctgctgacg acggctctgc tgttcaatgc catgcgcgac gtgtggcgct
     1441 ggccagtgat catcgctgtg ttagtcagcg gcgcgttcct gctgatcgac ctgaccttct
     1501 tcggcgccaa cctgatgaaa atccgagaag gcggctggat cccgctgctg ctgggcggcc
     1561 tggttttcgt cgtgatgacg acgtggcacc gcggcgtcga ggcggtgcgc cgcaccctgg
     1621 tgcagaagcc ggaaaccgcc gctgaatttc tggcccaact gaacaaaggc acgatcactc
     1681 gggtgcccgg gacggccgtt ttcctcagcc gcagcgagac ggccatcccg gcggtgatgg
     1741 tccgccatgt cgcacagatc aaggcgttgc aggagacggt ggtcagtctg accgtgcggt
     1801 tcgaggatta tcccagggtt ccgctggaaa agcgggcgga ggtcgagaaa atagcggaag
     1861 gcttatggca tgtgacaatc cgcttcggct ttgtcgagat gccgaacgtc gccgcggcac
     1921 tcgcctgtgc caaagagaaa ggatgtcagc tcaatctgga tgatgcggtt tacttcgccg
     1981 cgcatgacga tgtggtacgc agcgccaatc cgcctcggct ggcggcatgg cggcgcatgc
     2041 tgttcgcgct gatgtaccgc aacgccgtcc gcgcaccgga ccggtttgag ctgccagccg
     2101 acaagttcct ggaggtcggg cggcagattg ctttgtagcg ggccatttag gtagtggacc
     2161 atctagggtt tcggccgaat ccagttggag cgaacccatc ccactggtcg cggcctccca
     2221 cagcctgtaa ggttaccgca accataactg ggggaagaac gcgatgacca ccgccaccct
     2281 gtcgcgccgg atgatgttgt ccgccatcgc ggccagcccc gtcgccatgg atttcctgcg
     2341 cggtgccatg gcggaggcgg ctgacctgac tgcctcgggc ctcgttggcg agctgcaagg
     2401 cccgaccatg atcgtcgatc ccgcgaagtg gcccaagaca ttccacgaag cacccatgct
     2461 cgcggagcag gtcaaggcgg gcaagctgcc gccggtggcc gagcgcgtgc cggccgaacc
     2521 aatggtctgg cagccactga atgaggtcgg caaatatggc ggcacctggc ggcgcgcttt
     2581 taccggaccg gcggacggcg agaacggcaa ccggatccag tcctccgaca agccgctgca
     2641 ctggagcgcc gacggcagca agatcgtccc ctgcgtcgcc aagggctatg cgctcagtga
     2701 cgacggcaag acctacactt tgttcctgcg gaagggcatg aagtggtcgg acggcaccgc
     2761 gttcaccgct gacgatttca tcttctggta cgaagacatc tacggcaacc cagagatcgt
     2821 tcccacgccc acgccggaaa tgcgtccgcg ggggaagccc ggccgcatgg tcaaggtgga
     2881 cgaaaccacg gtgcgctggg aattcgatgt cccgtactat ctgttcgagg acatcatggc
     2941 cggcgatacg gcgatgggcg gcggacaggc ggtgcgccag gctgccaagg tcagcttcgg
     3001 tggctatgcg ccggcacact acctcaagca gtttctgccg aagtattcct cggtggaggc
     3061 agcgaacgcc cgtgccaagc aggaaggttt cgagaactgg gtgcagatgc tgcacttcaa
     3121 gaaggactgg gagctgaata tcgaggtgcc cgtcctgggg ccatggcgca cggtgcggcc
     3181 gatcaacacc gcgatctggc tgctggagcg caatccttac tactatgccg tggacacggc
     3241 gggtaatcag ttgccgtatt tcgaccgggt ccagttcacg ctcgcggaga acctggagat
     3301 catcaatctc cgggcgatgg cgggcgaata cgacgagcaa gagcgtcata tcgatctggg
     3361 caagttaccg gtgctgctgg ataaccagga gaagggcaac tacaaagtac acctcgatct
     3421 gggcttcgcc ggatcggatt tcatgttcca cgtcaaccag agtttcaccg gcgacgcaga
     3481 gatccgcaaa tggctgacca atgccgattt ccgccgggcg ctgtcgctgg gcatcgaccg
     3541 cgaccagttg aacgagacgt tctggctggg cgtcggcacg ccgggatcgc ccgctccagg
     3601 cgagagcatg ccgcaaagtc ccggggcgga ttggcgaaag aagtggtcca cgtacgaccc
     3661 ggcgcttgcc aacaagatgc tggacggcat tgggctggcg aaaaaggata atgagggttt
     3721 ccgcttgcgc accgacagcg gcgagcgcct gcgcatccag gtgatcgcga tcgccgcgtt
     3781 tctgccctat ccgaagctgt ccgagatggg tgcggaccag tggcgcaaga tcggcatcca
     3841 ggcagatgtc cgcgagatgg agcgaggact tggcttcgcc aaacaaacca acaatga
//
LOCUS       DASWQN010000891         5536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1405261, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000891 DASWQN010000000
VERSION     DASWQN010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405261"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VGC82_15115"
     CDS             <1..1052
                     /locus_tag="VGC82_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0424647.1"
                     /translation="HRSYPVGHLSTMYALGLLAGDIHLNVSSPGWAKHAWSCFFAPWN
                     VGAAVFIANQGRFNARTMLDTIATHKVTTLCAPPTVWRMLIQEDLRSIQHSLREVLGA
                     GEPLNPEVIDQVKAAWGLTIRDFYGQTETSAQVGNPPGQPIKPGSMGRPLPGFRTLLL
                     DADDHEADEGELSLALDPRPAGLMRGYQQEDGSISGLTGAAYRTGDVAFRDADGYLTY
                     VGRADDVFKASDYRISPFELESVLIEHPAVAEAAVVPAPDKLRLAIPKAYIALAAGAT
                     GDRATALAIFQHLRERLAPYKRIRRLEFYDLPKTISGKIRRVELRQMEAQRAAGSVRE
                     PAEFREEDFAELTAR"
     gene            complement(1215..2207)
                     /locus_tag="VGC82_15120"
     CDS             complement(1215..2207)
                     /locus_tag="VGC82_15120"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY0424648.1"
                     /translation="MSAMPKAELHLHIEGTLEPEMMMRLAERNRVALPYADVEAIQAA
                     YRFSSLQDFLELYFVGMSVLRTRDDFFDLAFAYLEKIAAQHVTHVEIFFDPQGHTERG
                     ISFDTVLSGLTDALDKARRDFGITSKLIMCFLRHLPEDQAFETLRHACRCKEHIFAIG
                     LDSTEKNHPPSKFARVFAEARREGFVPVAHAGEEGPPAYVSEAIDILRVQRIDHGNRA
                     LEDPALTVRIARQRIPMTVCPLSNRALQSCPDLTLHPLRRMLEQGLLVTVNSDDPSYF
                     GGYVNENFVAIQEALALTQHEIVTLARNSFLASFLPEQEKLAALNRFDVFQEKW"
     gene            complement(2358..2732)
                     /locus_tag="VGC82_15125"
     CDS             complement(2358..2732)
                     /locus_tag="VGC82_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424649.1"
                     /translation="MRALVLTASLLVAMTAAGFTQPYAVGGAAIPPAAAPQGENPSDY
                     LRAAQGALAAGRTREAQEALEMAQTRLLDRSVPQGQTNSPSDSPAVGQISEALRALAA
                     RDRASCLQLIQAAITSAAASGL"
     gene            complement(2906..3799)
                     /locus_tag="VGC82_15130"
     CDS             complement(2906..3799)
                     /locus_tag="VGC82_15130"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEY0424650.1"
                     /translation="MRRQLNLYGERILGRLVKWLIRLLRRTDPDRASDFCGAAARRIG
                     PLLPAHRIGQANLRAAFPDKDPVWIETTLRDAWDNLGRVAGEYVHMARIWDFDLDHPN
                     TGRIQTDSVSLFEMLRTDGKPGLCFAAHLANWELPAIAAAAHGLPSAVVYRMPNNKAV
                     AKEIIRIRAPLMGRLIRTRAQAAIEMAASLSAGEHLGMVVDQHFSRGVDVTFFGRTCK
                     ANPSIARLARGFDCPVVGVRVIRLPGHRFRIDGFGPYELPRSSNGQVDVQATTQMISD
                     VIEGWVRENPGQYLWFHRRWR"
     gene            complement(3805..4836)
                     /locus_tag="VGC82_15135"
     CDS             complement(3805..4836)
                     /locus_tag="VGC82_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0424651.1"
                     /translation="MATISALRLLGDRQVALVDREAPPPPGPDEAQLRVRVVGLNHID
                     VWGFRGMAFAKRLYPLTVGAEASCEVVAVGADVTNFRPGDRVVPYSALTCGICPACVR
                     GRDNLCENVSGVRGFHIDGFAEELTNHPARLLVKIPDGVSMHDAACVAVAYGTVEHML
                     FDNAHLEPGEWVLVHAGGSGIGSIAIRIAKSIGCTVITTVGSAAKAEKAKALGADHVI
                     NYREDRFEGVVRKLTRKKGVDVVFEHTGADTWAGSLLSMKRGGRLVTCGATSGPTASL
                     NLMQLFQQQYRITGSFGAPMSALARALERIASGVKPVIDTVCGLPDFRAGVDRLETRD
                     VFGKILIEL"
     gene            complement(5152..>5536)
                     /locus_tag="VGC82_15140"
     CDS             complement(5152..>5536)
                     /locus_tag="VGC82_15140"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0424652.1"
                     /translation="VVYPSLEIIETRGPFSEQIALALADNAQQKTVILGAPSALPADL
                     TAIEARVFINGEQVAIGTGDAVLGNPLNSIVWLAGKLGAYDRGLKAGEIVMTGSFTRQ
                     FSITPGDKIETKFSGLGCVATAMSA"
BASE COUNT         1096 a         1716 c         1708 g         1016 t
ORIGIN      
        1 cacatcggtc gtacccggtc gggcacctct cgacgatgta tgcgctgggg ctgctcgcgg
       61 gggacatcca cctgaatgtt tcctcgcccg gctgggcaaa acacgcatgg agctgctttt
      121 tcgccccctg gaatgtcggg gccgcagtgt tcatcgccaa tcaaggccgc ttcaacgcca
      181 ggaccatgct tgacaccatc gcgacgcaca aggtcacgac gctctgcgcg ccacccacgg
      241 tatggcgcat gctgattcag gaagacctga ggtccatcca gcacagcctg cgcgaggttc
      301 tcggcgccgg cgaaccgttg aacccggagg tcatcgatca agtcaaggcc gcctggggtc
      361 tcaccattcg cgatttctac ggccagaccg agaccagtgc ccaggtcggc aatccgcctg
      421 gtcaaccgat caaacccggc tcgatgggcc ggccgctgcc cggctttcgg acactgctgc
      481 tggacgcgga cgatcacgaa gccgacgaag gcgaactttc gctcgccctc gacccgcggc
      541 cggccggatt gatgcgcggc tatcagcagg aagacggctc gatttctggt ttgaccggag
      601 cggcttaccg caccggcgac gtcgccttcc gcgatgccga cggctacctg acttatgtcg
      661 gccgagcgga cgacgtgttc aaagcctcgg actatcggat cagcccgttc gaactcgaaa
      721 gcgtgctcat cgagcatcca gcggtagccg aggctgcggt ggttccggcg cccgacaagt
      781 tgcggctggc tatccctaag gcatacatcg cgttggctgc cggagcgacg ggcgatcggg
      841 ctacggcgct ggccatcttc cagcacctgc gtgagcgcct ggctccgtat aagcgcatac
      901 ggcgattgga gttttacgac ttgccgaaga ccatttcggg caagatcagg cgggtcgaat
      961 tacggcagat ggaggcccag cgcgccgccg gcagcgtccg tgaaccagcg gagttccggg
     1021 aggaagactt tgcggagttg acggcgcggt aggcgttacc ggctgacaca tccgcgtcag
     1081 ggtctgaatg tcgtggtggc cgaagggcca tcttccacgg tcttagttat acgagcggaa
     1141 ggcgtggatg ccgaccttcg tcggcctgac gaggtgatgc cgcctgcgca ccggtctcaa
     1201 tgcaggttgg catgtcacca cttttcctga aacacgtcga acctgttcag cgcggcgagt
     1261 ttttcctgct ccggcaggaa cgaggccagg aacgaattgc gtgccagcgt gacgatctca
     1321 tgctgtgtca gtgcaagtgc ttcctggatg gcgacaaagt tctcgttcac atagccgccg
     1381 aaatacgatg gatcgtcgga gttgacggtg accagaagac cctgttccag catccgccgc
     1441 aacggatgca acgtcagatc cgggcatgac tgcagcgccc gattcgacaa tgggcagacg
     1501 gtcatcggaa tccgctggcg cgcgatccga accgtcagtg ccgggtcctc gagcgcgcgg
     1561 ttgccgtgat cgatgcgctg tacgcgcagg atatcgatcg cctcgctcac ataggccggg
     1621 gggccttcct cgccggcatg tgcgacgggc acgaaaccct cccggcgcgc ctcggcgaag
     1681 acgcgggcga acttcgacgg cggatggttt ttctccgtcg agtcaagccc aatggcgaag
     1741 atatgctcct tgcaccggca agcgtgccga agcgtctcaa acgcctgatc ttccggcaaa
     1801 tggcgcagga agcacatgat gagcttggag gtaatgccga agtcgcgcct cgctttgtcc
     1861 agcgcgtcgg tcagtccgga cagcacggta tcgaacgata tcccgcgttc ggtgtgccct
     1921 tgcggatcaa agaaaatctc cacatgcgtg acgtgctgtg ctgcgatttt ttccaggtaa
     1981 gcgaatgcga ggtcgaaaaa gtcgtcgcgg gttcgaagca ccgacatacc gacaaagtaa
     2041 agctccagaa aatcctgcag cgagctgaac ctgtacgcgg cctggatcgc ttcgacgtcg
     2101 gcgtaaggca atgcgaccct gttccgttcg gcgagccgca tcatcatctc aggttccagc
     2161 gtaccctcga tgtgcaggtg cagctcggcc ttcggcatgg cggagataaa acgatggagg
     2221 cgcgcttgat cgataggtcg ggtccgttcg aacgttcgat gggtgcaaga tatcggacgg
     2281 tctatccggt cgcaaggtga tcaggcgacg ggtgatcatg cgccacatat cccggctctc
     2341 ggtcgataca gttggatcta caaacccgat gcggcagccg acgtgatcgc tgcctggatc
     2401 aactgcaggc agcttgcccg gtcgcgggcc gcaagagccc tgagtgcctc ggagatctgc
     2461 ccgaccgccg gactgtcgct agggctattg gtctgtcctt gcggcacgga ccggtcgagc
     2521 aaccttgtct gcgccatctc cagagcttcc tgcgcctcgc gcgtgcgtcc ggctgccagc
     2581 gcgccttgtg ccgcacgcag atagtcggat gggttttcgc cttgcggagc ggccgcgggt
     2641 gggatcgctg ctccgcccac cgcgtaaggc tgggtaaacc ccgccgccgt cattgcgacc
     2701 agcaacgacg ccgttaaaac gagagcacgc attgaatccg atctcctgag cgatggttcc
     2761 gataccacaa tttacagcat aaccacgccc ggcaaaacgt tcgaagcgtg tgaatgtgcg
     2821 atcatacatg gagttccact ttcaacctgc acagcgaggc tgaaagtgga cgagggagtt
     2881 gttgcttctt gcgccacggg cccgatcaac gccaacggcg atgaaaccat agatactggc
     2941 cgggattttc gcgcacccat ccctcgatga cgtcgcttat catctgcgtc gtggcctgca
     3001 catcgacttg cccattgctg gatctcggca gttcatatgg cccaaagccg tcgatccgaa
     3061 aacgatgacc cggcaagcga atgacccgaa ccccgacgac cggacaatcg aagccacggg
     3121 cgaggcgcgc gatcgatggg ttggccttgc aggtccggcc gaagaacgtg acgtccacgc
     3181 cgcgggagaa atgctggtcc acgaccatac ccaaatgctc gccggcggac agcgaagctg
     3241 ccatttcgat cgccgcctgg gcacgagtac ggatcagccg ccccatcagc ggggcgcgaa
     3301 tgcggatgat ctccttcgcc accgccttgt tgttcggcat ccggtagacg acggccgacg
     3361 gcagcccatg cgccgcggcg gcgatggcgg gcagttccca gttcgccaaa tgggcggcga
     3421 agcacaatcc cggtttcccg tcggttcgca gcatttcgaa caaggaaacg ctgtccgtct
     3481 ggatccggcc ggtattggga tgatcgaggt cgaagtccca gatgcgcgcc atatgcacat
     3541 actcgccggc aacgcggcca agattatccc atgcatcccg caaggtggtt tcaatccaaa
     3601 ctgggtcctt gtcgggaaag gcggcgcgca ggttggcttg gccaatgcga tgagccggca
     3661 gcagcgggcc gatccgccgc gccgccgcac cgcaaaaatc cgatgcccga tcgggatcgg
     3721 tgcggcgcaa taagcggatc agccacttca ccagccggcc gaggatacgc tccccgtaaa
     3781 ggttcagttg gcggcgcacg ccgatcagag ttcgatcagg atcttgccaa aaacatcccg
     3841 ggtttccagc cggtccacgc cggctcggaa atccggcagg ccgcaaaccg tgtcgatcac
     3901 cggcttcacc ccggatgcga tccgttccag cgcgcgcgcc agggctgaca ttggtgcgcc
     3961 aaacgacccg gtgatgcggt attgctgctg aaacaactgc atcaggttca ggctggcggt
     4021 aggacccgag gtggcgccgc aggtgaccag ccggccgccg cgcttcatcg agagcaagga
     4081 acccgcccag gtgtcggcgc cggtatgctc gaagacgaca tccacgccct tcttcctggt
     4141 cagcttgcgc acgacgccct cgaaccggtc ttcgcggtag ttgatgacat gatccgcgcc
     4201 cagagctttg gccttctccg ccttggccgc cgaaccgacc gtcgtgatca cggtgcagcc
     4261 gatggatttt gcgatgcgga ttgcgatgct gccgataccg gagccgccgg catgcaccag
     4321 cacccattcg ccaggttcca gatgagcatt gtcgaacagc atgtgttcga cggtgccgta
     4381 ggcgacggca acgcatgcgg catcgtgcat ggaaacgcca tcgggaattt tgaccagcag
     4441 gcgggcgggg tggttcgtga gttcctcggc gaagccatcg atgtgaaagc cacgaacgcc
     4501 ggaaacgttt tcgcacaggt tgtcccggcc acgaacgcag gcggggcaga tgccgcaggt
     4561 cagcgcggaa tacggcacca cgcggtcgcc cggccgaaag ttggtcacgt ccgcgccgac
     4621 cgccacgacc tcgcaggatg cttcggcgcc aacggtcagc gggtagagcc gcttggcgaa
     4681 ggccatgccg cgaaagcccc agacgtcgat gtggttcaag cccaccaccc ggacgcgaag
     4741 ttgtgcttcg tccggaccgg gcggcggcgg ggcctcgcga tccaccagcg cgacctgacg
     4801 gtcgccaaga agccggagag cgctaatcgt cgccatcgag tcgggatcct gaagggttgg
     4861 gtggctgccc agtaagcacg ctttgtcgtc agggcaatcc ccgttcgaac attctgaacc
     4921 ggtctatcga caccacgggc attaacaggt cgttcaggta tgccgactaa aacgcaaaag
     4981 ccggacggga ataggcccgt ccggcttact cgcggagaat gagatgttcc tagctctcaa
     5041 actcttaccg aggatagtgg tgaaaattgg cctaaaagtc aagatcatca tcatccgaaa
     5101 gtaggaagtt ggctggcccc atgcttcggg ccagccgctt ccgcaaggac attatgccga
     5161 catcgctgtc gccacacagc caagcccgga gaacttcgtt tcgatcttgt cgccgggcgt
     5221 gatcgaaaac tggcgcgtga acgacccggt catgacgatc tccccggcct ttaggcctcg
     5281 gtcataagcg cccagcttgc cagccagcca tacgatcgag ttcaaagggt tgcccagaac
     5341 agcatccccc gtgcctatcg ccacttgctc gccgttgatg aaaacccgag cttcgatcgc
     5401 tgtcagatcg gcgggcaacg cggagggagc acccaggatg actgtctttt gttgcgcatt
     5461 gtcggccaaa gccagtgcga tctgctcgct gaaaggtccg cgggtttcga tgatttccag
     5521 cgatggatag accacg
//
LOCUS       DASWQN010000892         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2593275, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000892 DASWQN010000000
VERSION     DASWQN010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2593275"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /gene="proB"
                     /locus_tag="VGC82_15145"
     CDS             complement(<1..1045)
                     /gene="proB"
                     /locus_tag="VGC82_15145"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY0424653.1"
                     /translation="MIPSLEKSQRVVVKIGSALVVDETQAATRTSWLDSVAADLQVLR
                     QRGTDVIVVSSGAIALARRTLGLTQKRLRLEEKQAAAAVGQIRLAQAWAQALSARELT
                     AAQLLLTLDDTEDRRRYLNARETLNTLLALHCIPVINENDTVATAEIRFGDNDRLAAR
                     VAEMVQADQLILLSDIDGLYTADPRRNPQASHIPVVESITAEIEAMGGDPPPGYSSGG
                     MRTKLVAARIATQAGCAMAIAIGNVERPLQALERGARCTWFLPVPEGRTARKRWIAGS
                     LSALGILTVDAGAAKALASGRSLLPAGVKAVEGLFERGDPVMIVGTDGITLGRGLSAY
                     ASTDAERIVGRRSD"
     gene            complement(1042..>1935)
                     /gene="obgE"
                     /locus_tag="VGC82_15150"
     CDS             complement(1042..>1935)
                     /gene="obgE"
                     /locus_tag="VGC82_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY0424654.1"
                     /translation="IIFVAEHNLNTLIDFRYTQHFRAPKGGNGAGSDRTGAGARDVVI
                     IVPVGTQVFADDQETMLADLDVAGKRVTLLRGGDGGFGNAHFKTSTNRAPRKMTKGWP
                     GEERWVWLRLKLIADAGLVGLPNAGKSTFLSVVSAAKPKIADYPFTTLHPQLGVIRLS
                     NSEEFVLADIPGLIEGAHEGAGLGDRFLGHVERCAVLLHLIDGAAGDVVKTWRTVRDE
                     MHAYGGGLAEKPEIIGLNKSDAMTPRETSSRLAALRKASARPVYLLSGATGQGVAEVL
                     RVMQDTIHATRREKAAPAEVA"
BASE COUNT          327 a          605 c          654 g          349 t
ORIGIN      
        1 ggtctgaacg gcggccgaca atacgttcgg catcggtgct ggcataggcg gacaggccac
       61 ggccaagcgt gatgccatcc gtcccgacga tcatgacagg atcaccgcgt tcgaacagac
      121 cctcgaccgc cttgacgccg gccggcagca aggatcggcc gctcgccagg gctttggccg
      181 cgccggcatc gacggtcagg ataccaaggg cagaaagcga cccggcgatc cagcgtttgc
      241 gggcggtccg gccttcgggc accggcagga accaagtgca gcgggcgccg cgttcaagcg
      301 cttgcagcgg cctttccacg tttcctatgg cgatggccat ggcgcagccg gcctgggtcg
      361 cgatgcgggc agcgacaagt ttggtacgca tgccgccgga cgagtaaccg ggcggtggat
      421 cgcctcccat cgcctcgatt tccgcagtga tggattcgac gaccgggatg tggctggctt
      481 gcgggtttcg ccgtggatcg gcggtgtaca agccgtcaat gtcggacaac aggatcagct
      541 ggtcggcctg gaccatttcg gcgaccctgg ccgcgagacg gtcgttgtcg ccgaaacgga
      601 tttccgccgt ggcgaccgtg tcattctcgt tgatgaccgg gatgcaatgc aaggccagca
      661 gggtgttgag ggtttcccgg gcgttgaggt atcggcggcg atcctcggtg tcgtccaatg
      721 tcaacagcaa ctgggccgcg gtcagctcac gcgcggacaa ggcctgggcc caggcctgcg
      781 ccaggcggat ctggccaacg gcggccgcgg cctgtttttc ttccagtctc agacgtttct
      841 gggtgagccc cagcgttcgc ctggccaggg cgatggcacc ggatgaaacg acgatgacat
      901 cggtgccgcg ctggcgcagt acctggaggt cggcggccac gctatccagc catgatgtgc
      961 gagttgcggc ctgggtttcg tccacgacca gcgcactacc gatctttacg acgacgcgct
     1021 gcgatttctc aagcgatggg atcacgccac ttccgctggc gcggcctttt cacgccttgt
     1081 ggcatggatc gtgtcctgca tgacccgcag cacctccgcc acgccttggc cggtggcccc
     1141 ggacagcaga tagactggcc gagcggaagc ttttcgcagc gccgcgaggc gagacgacgt
     1201 ctccctcggg gtcatggcat cggatttgtt caggccaatg atttcgggtt tttccgccag
     1261 tccgccgcca tacgcgtgca tctcgtcgcg gacggtgcgc caggtcttga ccacgtcgcc
     1321 cgcggcgccg tcgatcaggt gcagcagcac ggcgcagcgc tcgacatggc cgagaaaacg
     1381 atcgcccagg ccggcgcctt catgggcacc ctcgatcagg ccggggatat cggcgagcac
     1441 gaactcctcg gagttcgaca ggcggatgac gccgagctgc ggatgcaggg tggtgaaggg
     1501 gtagtcggcg atcttgggct tggccgcgga aacaaccgaa aggaacgtgg atttgccggc
     1561 gttcggcagc ccaaccagac ccgcgtccgc gatcagcttc aggcgtagcc agacccagcg
     1621 ttcctcgccg ggccagccct tggtcatttt gcgcggcgcg cgattggtgg aggtcttaaa
     1681 gtgcgcattg ccaaagccgc cgtcgccgcc gcgcaacagg gtgacccgct tgcccgcgac
     1741 atcgaggtcg gcgagcatgg tttcctgatc gtcggcaaag acctgggttc cgacagggac
     1801 gatgatcacg acatcacggg ccccggctcc cgtccggtcc gagccagcgc cgttgccgcc
     1861 tttcggagct cggaaatgct gggtgtagcg aaagtcgatt agcgtgttca ggttgtgttc
     1921 ggcgacgaaa atgat
//
LOCUS       DASWQN010000893         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_217644, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000893 DASWQN010000000
VERSION     DASWQN010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217644"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..1715)
                     /gene="hslU"
                     /locus_tag="VGC82_15155"
     CDS             complement(393..1715)
                     /gene="hslU"
                     /locus_tag="VGC82_15155"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003628148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY0424655.1"
                     /translation="MDVPTYSPREIVSELDRFIVGQDDAKRAVAIAMRNRWRRQQLPE
                     GMREEVVPKNILMIGPTGCGKTEIARRLAKLAQAPFLKVEATKFTEVGYVGRDVESIV
                     RDLVDASLNMLRESSRKGVQAKAEAAAEERLITALVGDEAAADTRSKFRRMLRNGEFE
                     TKEIEVSVSDAASSPIGQLDMPGMPPGQVINLSEMMKGLMGNRPQQKRRMTVEAARRI
                     LEAEEADHLLDNDQLSKDAVSHAENNGIVFLDEIDKICARTSEGGFRGGDVSREGVQR
                     DLLPLIEGTTVNTKYGLVKTDHILFIASGAFHLAKPSDLLPELQGRLPIRVELLPLSR
                     ADLRRILVEPEHSLLKQYAALLGTENVVLDFRDDAVDALADLASDINDRIENIGARRL
                     HTVLERLLEDISFTATDRGGETFVVDAAYVAEKVAPLAQQGDLSRFIL"
     gene            complement(1717..2283)
                     /gene="hslV"
                     /locus_tag="VGC82_15160"
     CDS             complement(1717..2283)
                     /gene="hslV"
                     /locus_tag="VGC82_15160"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEY0424656.1"
                     /translation="MQNLSNAAADPIGWHGTTILCVRRDGKVTMAGDGQVSMGQTIVK
                     GNARKVRRIGAGGTVISGFAGATADAFTLLERLESKLERYPNQLERACVELAKDWRTD
                     RYLRRLEALMAVADKDRSFTLTGNGDVLEPEDGVIGIGSGGNYALAAARALMTIPDLS
                     AEEIARRSMRIAADICVYTNGNTIIESL"
BASE COUNT          432 a          787 c          838 g          523 t
ORIGIN      
        1 agaccccgga tgcggccgtt cggggtgagg agtattccgg ggtgcatgag tcacatggag
       61 ccgtggaggc tacagctacg ttatcgctgg ttgggaaacc aacgacttcc tggtctctct
      121 tgccgctgaa ctggaaccga agttcgagtt gagcttgtag ggaacggaca tctctggctg
      181 acgatgttct ggcggtcagg gctgggatcg gccgtaatag cggttgatgt tactgccgtg
      241 cttgctggca acccgcgacc ctgttgatgc gggaaagtcg tgggtggccg cgccaagcac
      301 ggccatgaca caggggggat gaccctggca caggggggat cgggaccaga agcttatgac
      361 ggagcgtgga tgtcgggctg aacccgccga tactacagga tgaaccggct gaggtcgccc
      421 tgttgtgcca gtggggcgac cttctcggcc acataggcgg catcgacgac gaatgtttcg
      481 ccgccgcggt cggtcgcggt gaagctgata tcctccagca gccgctccag caccgtatgc
      541 aaccgcctcg caccgatgtt ttcgatgcgg tcgttgatgt cggaggcgag atcggccaac
      601 gcatccaccg catcgtcgcg gaagtcgagc accacgttct cggtacccag cagcgcggca
      661 tactgcttca gcagcgagtg ttcgggctcg accaggatgc gccgcaggtc ggcgcgtgac
      721 agcggcaaca attcgacgcg gatcggcagc cggccctgca gttccggcag caggtcggac
      781 ggcttggcca gatggaacgc gccggaagcg atgaacagga tgtggtcggt cttcaccagg
      841 ccgtacttgg tgttcacggt cgtgccctcg atcagcggca acaggtcgcg ttgcacgcct
      901 tcgcggctga cgtcgccgcc gcggaagccg ccttccgacg tgcgggcaca gatcttgtcg
      961 atctcgtcca ggaagacaat gccgttgttc tccgcgtgcg acaccgcgtc cttgctgagc
     1021 tgatcgttgt ccagcaggtg gtcggcttcc tcggcttcca ggatgcggcg agccgcttcg
     1081 accgtcatcc ggcgcttctg ctgcgggcgg ttgcccatca ggcccttcat catctcgctc
     1141 aggttgatga cctggcccgg cggcatgccc ggcatatcga gttggccgat gggcgaggaa
     1201 gccgcgtcgg acacggacac ctcgatttcc tttgtctcga actcgccgtt gcgcaacatg
     1261 cggcggaact tggaacgggt gtcggcggcg gcttcgtcac cgaccaaagc ggtgatcagg
     1321 cgttcttcgg cggcggcttc cgccttggcc tgaacaccct tgcggctgct ttcgcgcagc
     1381 atgttcaaac tggcatccac cagatcacgg acgatgcttt ccacgtcgcg gccgacatag
     1441 ccgacctcgg tgaacttcgt cgcttccacc ttcaggaacg gcgcctgcgc cagcttggcc
     1501 agccgacggg cgatttcggt cttgccgcag ccggtcgggc cgatcatcag gatattcttg
     1561 ggcacgacct cctcgcgcat gccttccggc aattgctgcc ggcgccagcg gttgcgcatc
     1621 gcgatcgcga cggcgcgctt ggcatcgtcc tggccgacga tgaagcggtc gagttccgag
     1681 acgatttctc tcggcgaata ggtagggaca tccatcttac agtgactcga ttatggtgtt
     1741 gccgttggtg tacacgcaga tatccgcggc gatcctcatc gatcgccgag cgatctcctc
     1801 ggccgaaagg tcggggatgg tcatcagcgc gcgggcggcg gcgagggcgt agttgccgcc
     1861 cgatccgatg ccgataaccc catcctccgg ctccagcaca tcgccgttgc cggtcagggt
     1921 gaagctgcgg tccttgtcgg caaccgccat cagcgcttcc aggcgccgga ggtagcggtc
     1981 ggtccgccag tccttggcca gttcgacaca ggcgcgttcc agttggttcg gataccgctc
     2041 cagcttgctt tccagccgtt cgagcagggt aaaggcgtcg gcggtcgcgc cggcgaaacc
     2101 ggagatgacg gtgccgcccg cgccgatgcg ccggactttc cgcgcgttac ccttgacgat
     2161 ggtttgtccc atgctgacct ggccgtcccc ggccatggtc actttgccat cccgccttac
     2221 gcacaggatg gtggtgccgt gccacccgat cgggtcggcg gcggcgttcg aaaggttctg
     2281 cataacggac caaggtggcg atccttgccc ggttgggcaa gagccatcgc gcagccgcct
     2341 catatgatcg cgaggcatga tcgcgttcgg ggctgggtca tacatgaatc ggaacaaaat
     2401 gcgggagcgc gatgccccat aaccaggtgt tatttcgcct gtccgcgacc gcatatgtta
     2461 tcaggcacgc ccgttggcat atgttatcag gcacgcctgt tgtcggtggt ctttcgacca
     2521 acgacatggt ttcaggggtt tctatggaag ttcagcacct gatgaagtgg ctatggctga
//
LOCUS       DASWQN010000894         4388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2460135, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000894 DASWQN010000000
VERSION     DASWQN010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2460135"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VGC82_15165"
     CDS             <1..540
                     /locus_tag="VGC82_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEY0424657.1"
                     /translation="CGAKFAIRGFTDSLRSEIIHDGLNISLSMVDLPAVNTPQFTWAL
                     NKTGRRAQPVPPIFQPEIPARAIYFAAFNPRRQTWIGFPTVKAILANRIAPGLLDRYL
                     ARAGYSGQLGPEPLPENAPNNLFLPVPGAYAAHGRFDDRARTMSWEVFTSRHRNAMWA
                     TVGLAAVIGLHQLARKLRI"
     gene            621..1085
                     /locus_tag="VGC82_15170"
     CDS             621..1085
                     /locus_tag="VGC82_15170"
                     /inference="COORDINATES: protein motif:HMM:NF014082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0424658.1"
                     /translation="MTDLNSDDDPRSGEVVVPLPAETNAGVYFIGTIHTPWHHRAECP
                     KRGSLDGPVCTIVVHERWWQALTGVGDHPRIQVLYWMHRARRDLVLQTPMRTGQTTGS
                     FALRSPVRPNPIASSVVALVGIEGGTLHVRGLDCLDGTPLIDLKPDRSTTAE"
     gene            complement(1134..2021)
                     /locus_tag="VGC82_15175"
     CDS             complement(1134..2021)
                     /locus_tag="VGC82_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0424659.1"
                     /translation="MVQVPGAQIPGVYHRRIGDIVVTALSDGYLDGTVEVMQNIAPNE
                     ATQMLADKFRPGRRTSVNCYLVYSAGRLALVETGSGDYLLPTAGKLQQNLKAAGVNPA
                     DIDTVILTHMHPDHSAGLTNPKTGEKFFPNAELVVHQNEPKHWQDDGAMSRADERART
                     MYFQCAREQMAPYHNQMRTFDSAVEVFPGVTSVPLHGHTPGHSGYMIASGDKSLLIWG
                     DIIHVPEVQVARPEVTMVFDTDPHAAAATRKRTFDMVATDKQLVAGMHIHFPGFAHVV
                     KQGDGYLLLPEPWDQGFGG"
     gene            complement(2060..2803)
                     /locus_tag="VGC82_15180"
     CDS             complement(2060..2803)
                     /locus_tag="VGC82_15180"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0424660.1"
                     /translation="MRLKDKVAIVTGGAHGMGEAEARLFAKEGAAVVIADMLTEQGEA
                     VAADIAGSQGRARFVRTDVTSETDWQKLISETIAAYGRLDILVNNAGISGSSVGDVDG
                     LEGWHKLLSVNAASVFLGTKLAAAAMMPNGRGSIVNISSIMGFVGSADSHPGYAASKG
                     AVRLYTKAAAAKYGPSGVRVNSVHPGYMPPMLNATNAVGRASKIEATPLRRLGEPIEV
                     AYGVLFLASDEASFITGTELVIDGGYIAQ"
     gene            complement(2834..4207)
                     /locus_tag="VGC82_15185"
     CDS             complement(2834..4207)
                     /locus_tag="VGC82_15185"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0424661.1"
                     /translation="MNAINDSQSGEGLTRALAEQARALRLEDIPQAVRAWARQCVLDY
                     IGCAVAGASDELVFMLLAELREQGGHEVASVLGHPERLPAPSAAMVNGASSHALDFDD
                     VNLAMPGHPSVAILPALLALAEERGSSGADVLTAFVAGYELQCRIGRTIAPGHYDVLG
                     FHATATVGSFGSAAACAHLLGLDAERFAMALGIAGTQAAGLKSMFGTMCKPLHAGKAA
                     YHGLMAAKLAARGFTSRPDVIECGQGFARTHSPDFHPDRAFEAPPGGWWIVNNLFKYH
                     ASCYMTHAAIEAARKLRGQHQFTADQVERIDVRLEESCDRICNIASPVTGLEAKFSLR
                     LTTAMGLAGVDTSRPSSYSEAVAADPVLIGLRDRVALDFRTGIPNTFSEIELSLRDGS
                     RFFARHDAGIPAADAVDQGKRLEAKFASLVDPVLGTETTARLIADIARLDLLADIGEL
                     MGFCVGR"
BASE COUNT          788 a         1484 c         1285 g          831 t
ORIGIN      
        1 tgcggcgcca aattcgctat aaggggcttt accgactcgc tgcggtcgga gatcattcac
       61 gatgggctga acatcagcct gtcgatggtg gatttgccgg ccgtcaacac gccgcagttc
      121 acttgggcac tgaacaagac gggccggcga gcccagccgg tgccgcccat tttccagccc
      181 gagattccgg cccgcgcgat ctattttgcc gccttcaacc cgcgccgcca gacctggatt
      241 gggtttccca cggtcaaggc catcctggcc aaccgcatcg cgccgggatt gctcgaccgc
      301 tacctcgcac gcgccggcta tagcggacaa ctggggcccg aaccgttgcc ggaaaatgcg
      361 ccgaacaacc tgtttctccc tgttcctggc gcgtacgcgg cgcatggccg cttcgacgac
      421 cgggcgagga ccatgagctg ggaggttttc accagccgtc atcgcaatgc gatgtgggcg
      481 accgttggcc tggccgcggt gatcggtctc caccagcttg ccaggaagct acggatttaa
      541 ggattatcgt ggctttgtac ccagcgaaag cgggtggttg ccactggact tgtggcgaca
      601 tcgggtggca cggtcgcgcc atgacagacc tgaactccga tgacgatccg cgatcgggcg
      661 aggtcgtggt cccgcttccc gcagaaacca atgccggtgt ctatttcatc ggcaccatcc
      721 acacgccctg gcaccaccgt gcggaatgcc ccaagcgcgg cagtctcgat ggcccggtat
      781 gcaccatcgt ggtgcatgag cgatggtggc aggcgctgac gggtgttggt gatcatccgc
      841 gcatccaggt gctgtactgg atgcatcggg cgcgccggga cctggttttg cagacgccga
      901 tgcgcacggg acaaaccacc ggaagcttcg ccctacgctc ccccgtgcgg ccaaatccga
      961 tcgcgtcgtc ggtcgtcgcg ttggttggga tcgagggcgg cacactgcat gtccgcggcc
     1021 tggactgtct ggacggcacg ccgctcatcg atctgaaacc tgatcgcagc acgaccgcgg
     1081 aatagccccc cgacaatcgg gtggaaccga cctgaaaccg ctcggttcgc gggtcagccg
     1141 ccaaagcctt gatcccacgg ttccggcagc agcaggtagc cgtcgccctg tttcaccaca
     1201 tgcgcgaagc cgggaaagtg gatgtgcatt ccagcgacca actgcttgtc ggttgccacc
     1261 atgtcgaacg tccgcttgcg cgtcgcagcg gcggcatgcg gatcggtatc gaataccatc
     1321 gtgacctccg gacgcgcgac ctgaacctcc gggacatgga tgatgtcgcc ccagatcagc
     1381 aacgacttgt cgccggaggc gatcatgtat ccggaatggc ctggcgtatg cccgtgcagc
     1441 ggcactgaag tcacgccagg aaacacctcg acggcactgt cgaatgtccg catctggttg
     1501 tggtacggcg ccatctgctc ccgagcgcac tggaagtaca tcgtcctggc gcgttcgtcg
     1561 gctcggctca tggcgccgtc gtcctgccag tgctttggtt cgttttgatg caccaccaac
     1621 tcggcattcg ggaagaactt ttcgccggtc ttcgggttcg tcagaccggc ggaatggtcg
     1681 gggtgcatgt gagtcagtat taccgtgtcg atgtccgcgg ggttgacgcc tgccgccttc
     1741 aggttttgtt gcagtttgcc cgccgtcggc agcaggtaat cgccggaccc cgtctccacc
     1801 agcgccaagc ggccggcgga atagaccagg tagcagttga ccgacgtgcg ccggcccggc
     1861 cggaatttat ccgctagcat ctgtgtggct tcgttcgggg cgatgttctg catcacctcg
     1921 accgtgccat ccaagtagcc gtccgacagg gctgtcacga cgatatcgcc gatccgccgg
     1981 tgatacaccc cggggatctg cgcgcctgga acctgaacca tgcaagacac tcctgttact
     2041 ggccctgttg ccgggcccct tattgcgcga tgtatccgcc gtcgatgacc agttccgtcc
     2101 cggtgatgaa cgatgcctcg tcggacgcca ggaacagcac gccgtatgcc acctcgatcg
     2161 gttcgcccag gcgacgcagc ggcgtggcct cgattttgga ggcgcgtccg acggcattcg
     2221 ttgcgttcag catcggcggc atgtagcccg gatgtaccga gttcacccga acgccggatg
     2281 ggccatactt ggccgccgcc gctttggtgt agagccgtac ggcacctttg gacgctgcgt
     2341 agccgggatg gctgtccgcg ctaccgacga accccatgat ggaggagata ttgacgatcg
     2401 accctcgccc gttgggcatc attgccgcag cggcaagctt ggtgccgaga aacacgctgg
     2461 cggcgttcac ggacagcaat ttgtgccagc cttccagccc atccacatcg ccaaccgaac
     2521 tgccgctgat cccggcgttg ttgaccagga tatcgagccg gccataggcc gcgatcgttt
     2581 cggagatcag cttttgccag tcggtttccg acgttacatc ggtgcggaca aaccgcgccc
     2641 gcccctgact cccagcaata tccgccgcca cggcttcccc ctgctcggtc agcatgtcgg
     2701 cgataacgac ggcggctcct tccttggcga acaggcgagc ctcggcttcg cccatcccat
     2761 gcgcgccgcc ggtaacgatg gcaaccttgt ctttcagacg cattttcccc tccattatcg
     2821 tgcagcgact ggatcaccga cccacgcaaa atcccatcaa ttcgccaata tccgccagca
     2881 aatccaaccg ggcgatatcc gcaatcaaac gggcagttgt ctccgtcccc aacaccggat
     2941 cgactaggga agcaaacttc gcctccagcc gttttccttg atccacggca tccgctgccg
     3001 gaatcccggc gtcgtgccgc gcgaagaacc gcgagccatc ccgcaacgac agctcgatct
     3061 cggaaaacgt gttcgggatc ccggtgcgga aatccagcgc aacgcggtcg cgcagcccga
     3121 tcagcaccgg atccgccgcg accgcctcgg agtagcttga cggacggctc gtatcgacac
     3181 ccgccagtcc catcgccgtg gtcaggcgca gcgagaattt cgcctccaag ccggtgaccg
     3241 gagacgcgat gttgcagatc ctgtcgcagg attcttccag ccgcacgtcg atccgctcaa
     3301 cctggtcggc ggtaaactga tgctgtcccc gcagctttcg cgccgcctcg atcgcggcgt
     3361 gggtcatgta acaagaggca tgatacttga atagattgtt gacgatccac cacccgccgg
     3421 gcggcgcctc gaacgcgcgg tccgggtgga agtccggact gtgggttctg gcgaacccct
     3481 gtccgcactc gatcacatcc ggccggctgg tgaaccccct cgccgccagt ttcgccgcca
     3541 tcagcccatg atacgcggcc tttccagcgt gcagcggctt gcacatcgtg ccgaacatcg
     3601 acttcagtcc ggccgcctga gtacccgcga ttcctagcgc catcgcgaag cgttccgcat
     3661 ccaggcccag caaatgcgca cacgccgccg ccgacccgaa gctgccgaca gtcgcggtcg
     3721 cgtggaatcc caggacatca taatgaccag gcgcgatcgt gcgcccgatc cggcactgca
     3781 attcatatcc cgcgacgaat gccgtcagga catcggcgcc gctcgaacct cgctcctccg
     3841 ccaacgccag cagcgccggc aggatcgcca cagacggatg tcccggcatc gccaggttca
     3901 catcgtcgaa gtccagggca tgcgaggacg ctccgttcac cattgccgca gaaggcgccg
     3961 gcaatcgttc tggatgaccc agaaccgagg cgacctcgtg gcccccctgt tcccgcagtt
     4021 ccgcaagcag cataaagacc agttcgtcgg atgccccggc gaccgcgcag ccgatgtagt
     4081 cgagtacgca ttgccgcgcc caggcccgca cggcctgtgg aatgtcttcc aaccgcaaag
     4141 ccctggcttg ttccgccaat gctcgcgtta gcccctcgcc cgactgacta tcgttgatag
     4201 cgttcatcgc cgatcgtccc attcatcgtt gtttatcacg tgattcacac gcatcgcacg
     4261 aacagcgcat cctttggacg acggacgttc cgcccagcgt gatcacgctc tagcgtccct
     4321 tgtaaaccgg cttccgcttc tcgttgaacg ccaggatgcc ctcgcgccgg tcgtccgtcg
     4381 ggaccatc
//
LOCUS       DASWQN010000895         4469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1536279, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000895 DASWQN010000000
VERSION     DASWQN010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536279"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..673)
                     /locus_tag="VGC82_15190"
     CDS             complement(128..673)
                     /locus_tag="VGC82_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEY0424662.1"
                     /translation="MDSMEINKVMAAVLIAGIMFFLTGLIGMHLVSVERLEKPAIAIQ
                     GAPAPEAAGGAPKQQALPPIAPLLAKADVAVGGATAKKLCTSCHTFTEGGKAGVGPNL
                     YGVVGAPHGHMEGFSYSAGMKAKTGPWTFDELNEWLHKPASYIPGTRMAFAGISDDQQ
                     RADVIAYLRSLAANPVSLPAP"
     gene            917..1669
                     /locus_tag="VGC82_15195"
     CDS             917..1669
                     /locus_tag="VGC82_15195"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019084895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEY0424663.1"
                     /translation="MNPIVVIPARMASTRLPGKPLADINGKPMIVHMLERGQEAGIGP
                     VAVACGDPEIAAAVRAAGGRAVLTDPALPSGSDRVHAALAELDPEGRHDIVVNLQGDL
                     PTLDPAGLRLVITPLTDPAIDIGTLVVPILDDAEAATASFVKAACAFQEGQTVALALY
                     FSRQAVPWGPGPRWHHVGIYAYRRAVLDRYVSLPPSPLERREGLEQLRALEDGMRIGC
                     VRMGHGPFGVDTEEDLERARSLLSGRAPLARS"
     gene            1666..2517
                     /locus_tag="VGC82_15200"
     CDS             1666..2517
                     /locus_tag="VGC82_15200"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY0424664.1"
                     /translation="MTGAISFQGVPGAYSDLACRNAYPAMATLPCPSFEAAIDAVRAG
                     QAALGMLPCENSLAGRVSDIHHLLPQSGLFIVGEQFQRVEHCLLGIKGATIGDIKRAH
                     SHTVALGQVRRILADLGLTPVVEADTAGAAQLVAEWGRKEDAAIASSLAAEIYGLQIL
                     RANVEDAAHNTTRFYVVAPKPAPVDPTTPNLMTTFVFRVRNVPAALYKALGGFATNGV
                     NMTKLESYMLGGEFAATQFLCDVDGHPEQPGLRRALDELLFFSTEVRVLGVYPMAAFR
                     LAQGAGG"
     gene            2592..3371
                     /locus_tag="VGC82_15205"
     CDS             2592..3371
                     /locus_tag="VGC82_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0424665.1"
                     /translation="MNDIRVEIHDRWRRLVLDRPSKLNALNTGMLTDLLAAIDAAEAD
                     AACRAVVLTGEGRGFCAGQELGADVLPGPEGPADLEALAGRYHHEVVRRMRGSRLPFI
                     CAVNGVAAGAGAGFALAGDIVLAARSATFVQAFIRIGLVPDSGASFFLPHLIGDARAR
                     ALAMLGDAIDAETAERWGMIWKTVDDAALASESAALALRLAKSPAGALAGIKQLFAAA
                     AVNSLHAQLDLEARFQGEAGRSADFSEGLLAFREKRAPRFQ"
     gene            complement(3365..3604)
                     /locus_tag="VGC82_15210"
     CDS             complement(3365..3604)
                     /locus_tag="VGC82_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424666.1"
                     /translation="MRGYRSFALVMTGLLLAGCTSDPDAAVNQTTPIRQMDNFFNSFS
                     AGSRPSDYSQRPSDYSRLPPDYTHEPGYAPAYPAY"
     gene            3704..4090
                     /locus_tag="VGC82_15215"
     CDS             3704..4090
                     /locus_tag="VGC82_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY0424667.1"
                     /translation="MASLDDTNDPTGRLIAANQVQGTTVYNTAKEKLGSVEDVMIDKA
                     SGRIAYAVLSFGGFLGIGDRYYPLPWEKLTYNTELGGYVVDVDRKTLEGAPSFTDDAA
                     ASWNDDAWSRDVYAHYGVHPYWDLMP"
     gene            4166..>4469
                     /locus_tag="VGC82_15220"
     CDS             4166..>4469
                     /locus_tag="VGC82_15220"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424668.1"
                     /translation="MKITRVETLQADAGWRMFSFLKITTSDGIVGWSEFNESFGSTGL
                     SGVIKGLSPMLIGRDPRRFEQVTQHLHVLTRQSRGGLNQQAIAAIENALLDVAGKAY"
BASE COUNT          773 a         1356 c         1523 g          817 t
ORIGIN      
        1 atggttaccg gcgaccggtc ggctttcagg tcggcagcga tggactggcg gaacaacggc
       61 cggataatcg cgccggccag gtcggcagcg gccactgccg ccgccacata tctgtcgaga
      121 tcgggactca aggcgcgggc agtgatacgg ggttggccgc caggctgcgc agataggcga
      181 tcacgtccgc ccgctgctga tcgtcgctga tgccggcgaa ggccatccgg gtgccgggga
      241 tataggaagc gggcttgtgc agccactcgt tcagctcgtc gaaggtccag ggcccggtct
      301 tcgctttcat ccccgcggaa taactgaaac cctccatgtg cccgtgcggc gcgccgacga
      361 cgccgtaaag gttcggaccg acacccgctt tgccgccctc ggtgaacgta tggcaggagg
      421 tgcacagctt cttcgcggtc gctccgccca cggccacatc agccttggcc agcagcggtg
      481 cgatcggcgg cagtgcctgc tgcttcggtg cgccgcccgc ggcctcaggc gccggagcac
      541 cctggatggc gatcgccggc ttctccaacc gttccaccga caccagatgc atgccgatca
      601 ggccggtgag aaagaacatg atgccggcga tcaggaccgc ggccatgacc ttgttgattt
      661 ccatgctgtc catgcggcca ggcttcctga tcgagcgagg caccgggcct cggatgaatg
      721 ggcgcaatcg aaggcgcctt cccgccgttg cacgggggcg agcgcatccg taagctggga
      781 caccatacgg aagcgaaatc gcctcttcaa cccacccgga agacgccttt gccggttcga
      841 cgtcggcgag gccgtccggc gggacatttc caggaagcct tttgcgttga acccgaccgc
      901 cgcgaaatcg cctgttctga atccgatcgt cgtgattccg gcccgcatgg cttcaacgcg
      961 cctgccgggc aaaccgctgg ccgatatcaa cggcaagcct atgatcgtgc atatgctgga
     1021 gcgcggccag gaagcgggca ttggcccggt ggccgtcgcc tgcggcgacc ccgagatcgc
     1081 cgccgccgtc cgcgcggcgg ggggacgagc ggtgctgacc gatccggcac tgccatcggg
     1141 ttcggaccgg gtgcatgcgg cactggccga actcgatccc gagggcaggc atgacatcgt
     1201 ggtcaacctg caaggcgatc ttccaacctt ggacccggca ggcctgcggc tggtgataac
     1261 cccactgacc gacccggcca tcgatatcgg caccctggtg gtgccgatcc tggacgacgc
     1321 cgaggccgcc accgcttcgt tcgtaaaagc cgcatgcgct ttccaggaag gccaaacggt
     1381 tgcgctggcc ttgtattttt ctcgccaggc ggttccctgg ggtccggggc cgcgctggca
     1441 tcatgttggt atctatgcct atcggcgcgc ggtgctggat cgctacgtgt cgctgccgcc
     1501 aagtccgctg gaacggcgcg aaggtctgga acaactgcgg gcgctggagg atgggatgcg
     1561 tattgggtgc gttcgaatgg gccatgggcc gtttggggtc gataccgagg aagacctgga
     1621 gcgcgcccgg tctttgctga gcggccgcgc gccgctggcc cgttcatgac cggcgccatc
     1681 tcgtttcaag gcgtgcccgg ggcatactcc gatctagcct gccgcaacgc gtatccggcc
     1741 atggccacgc tgccttgccc gtcgttcgag gcggcgatcg acgcagtgcg tgccggacag
     1801 gccgcgctgg gaatgctgcc gtgcgagaac agcctggcgg ggcgggtgtc ggatatccac
     1861 cacctgctgc cgcaatccgg cctcttcatc gtcggtgagc aattccaacg tgtcgaacac
     1921 tgtctgcttg gtatcaaagg tgccaccatc ggcgacatca agcgggccca ttcgcacacc
     1981 gtcgcgctgg gccaggtgcg gcggatcctg gccgatctgg ggctgacccc cgttgtcgag
     2041 gcagacaccg cgggcgcggc acagcttgtt gccgagtggg gacggaagga ggatgccgcc
     2101 atcgcctcgt cgctggcggc ggagatttat ggcctgcaga tcctgcgggc gaatgtggag
     2161 gatgcggcgc acaacacgac ccggttctat gtggtggcac cgaagccggc gccggtcgat
     2221 ccgacgaccc cgaacctgat gacgacgttc gtctttcgcg tccgcaacgt gccggctgca
     2281 ttgtacaagg cgctgggcgg gtttgccacg aacggcgtca acatgaccaa gctggaaagc
     2341 tacatgttgg gcggagagtt cgcggcgacc cagttcctgt gcgacgtcga tgggcacccg
     2401 gaacagcctg gattgcggcg ggcgttggac gagcttttgt tcttttccac cgaagtgcgg
     2461 gtgctgggcg tgtatccgat ggcggcgttc cgccttgccc agggcgccgg cggataacgc
     2521 ctgaactgcc cgcttgcagc ataagcgacg gggtcaacaa tgcgaagcgg aacgaacgga
     2581 gcttgtaacc gatgaacgat atccgggttg agatccatga tcgttggcgg cggctggtgc
     2641 tcgatcgtcc gtccaagctg aacgcgctca acacggggat gctgaccgac ttgttggcgg
     2701 cgatcgacgc cgcggaagcg gatgccgcct gccgagcggt ggtgctgacc ggggaaggcc
     2761 ggggattttg cgcgggccag gagttgggcg ccgatgtcct gccgggtccg gagggaccag
     2821 cggatctgga ggccctcgcc ggccgttacc atcatgaggt ggtgcggcgg atgcgcgggt
     2881 cgcggttgcc gttcatctgc gcggtcaatg gcgtcgcggc cggcgccggt gccggtttcg
     2941 cattggccgg cgacatcgtg ctggcggcac gatcggccac cttcgtgcag gcgtttatcc
     3001 ggatcggtct cgtgccggac agcggagcat cgttcttcct gcctcatctg attggtgacg
     3061 caagagcgcg ggcgctggcg atgctcggag atgcgatcga cgccgaaacc gccgaacgat
     3121 gggggatgat ctggaaaacg gtggacgacg cggcattggc atccgaaagc gcggcgcttg
     3181 cgctaagact ggcgaagtcc ccggccggtg ccctggccgg gatcaagcag ttgttcgcgg
     3241 cggccgcggt gaactcattg catgcacagc ttgacctcga agctcggttt cagggcgaag
     3301 cgggcaggtc ggcggacttc agcgagggct tgctggcgtt ccgggaaaag cgggcgcccc
     3361 ggtttcagta ggccggatag gcgggcgcgt agccaggttc gtgcgtgtaa tccggcggga
     3421 gccggctgta gtccgacggg cgctgactat aatccgatgg ccgggaacct gccgagaacg
     3481 agttgaaaaa attgtccatt tgtcggatcg gcgtggtctg gttgacggcc gcatccgggt
     3541 ccgaggtgca acccgccaac aacaatccgg tcatcacgag cgcgaaggat cggtaaccac
     3601 gcatggtcgt ctcctgacag aagatcaggt aactttcagg cgcgcccggc gtttcaagat
     3661 cgcgcagcta agttttaatg ttcgtagcca atgggagccg cacatggcga gtcttgacga
     3721 caccaacgat cccacgggtc gcctgatcgc tgccaaccag gttcagggca ccaccgttta
     3781 caacaccgca aaggagaagc ttgggtctgt cgaagacgtc atgatcgaca aggccagtgg
     3841 tagaattgcc tatgccgtgt tgagttttgg cgggttcctg ggtatcggtg accgttacta
     3901 tccgctgccg tgggagaaat tgacctacaa cacggagctg ggtggctacg tcgtcgatgt
     3961 cgatcgcaag acactggagg gtgccccgtc cttcactgac gacgcggccg cgtcttggaa
     4021 cgacgacgcc tggagtcgcg acgtttacgc ccattacggt gtgcatccct actgggacct
     4081 gatgccctga agcttgcccg ccgccgccgg gcgtgcttgg agtccctgtg gcgtgatagc
     4141 gcgatcatcc cgcccggggg gacagatgaa gattacacgc gtcgagacgc tgcaggctga
     4201 tgccggctgg cggatgtttt cattcctgaa aatcaccacc agcgacggga tcgtcggctg
     4261 gtcggagttc aacgaaagtt tcggtagtac aggcctgtcc ggtgtgatca agggattgtc
     4321 gccgatgctg atcgggcggg atccccggcg cttcgaacag gtgactcagc acctgcatgt
     4381 gctgacacga cagtcccgcg gcggattgaa ccagcaagcg attgccgcga tcgagaacgc
     4441 cctgctggac gtcgccggca aagcgtacg
//
LOCUS       DASWQN010000896         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2328559, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000896 DASWQN010000000
VERSION     DASWQN010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328559"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VGC82_15225"
     CDS             complement(<1..573)
                     /locus_tag="VGC82_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY0424669.1"
                     /translation="MDRVVDAVNLGTANGDLSLWGLFVQADIVVKLVMIGLLGASVWV
                     WAVVFEKWSTLRKVNKAADGFEDRFWSGGSLDELFDSEGSNPNHPMAAVFAAAMGEWR
                     RSVRVAGADISHTSVRERIDRAITVTVQREMDRVERWMVFLASVGATAPFIGLFGTVW
                     GIMHSFSAIAAMHNTNLAVVAPGIAEALFAT"
     gene            complement(577..1059)
                     /gene="ybgC"
                     /locus_tag="VGC82_15230"
     CDS             complement(577..1059)
                     /gene="ybgC"
                     /locus_tag="VGC82_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEY0424670.1"
                     /translation="MTTGPRSLHHYPLRVYYEDTDAGGVVYHANYLRYAERARTEALR
                     DAGIPHADLVERFRLMFMVHRAEIDYVRPAMLDDHLVVETETMDVGGATVSLRQTVRG
                     PNGVCATLRIKLACVRIGGNKPARIPPPWREAMAALPGKATPGAGKYHDQGAGAQQGD
                     "
     gene            complement(1143..>1863)
                     /gene="ruvB"
                     /locus_tag="VGC82_15235"
     CDS             complement(1143..>1863)
                     /gene="ruvB"
                     /locus_tag="VGC82_15235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY0424671.1"
                     /translation="VLFIDEIHRLQPAIEEILYPAMEDFQLDLIIGEGPAARSVRIDL
                     PPFTLVGATTRAGLLATPLRDRFGIPLRLVFYTPEELRQIIVRGARLLGLSLTGEGAA
                     EIAGRSRGTPRIAGRLLRRVRDFAAVQGDTPVDRAIADAALGRLEVDRLGLDSMDRRY
                     LRRIADHHAGGPVGVETLAAALAEARDTLEDVIEPYLIQEGLILRTSRGRMLGEPGWR
                     HLGLKPPRSGAPLLDLLGGEE"
BASE COUNT          330 a          625 c          603 g          305 t
ORIGIN      
        1 ggtggcgaac aaggcctcgg cgatgccggg agcaacgacg gcaaggttgg tgttgtgcat
       61 ggcggcgatg gcggagaagg aatgcatgat gccccagacg gtgccgaaca ggccgatgaa
      121 cggggcggta gcgccgacac tggcgaggaa caccatccac cgttccacgc ggtccatttc
      181 tcgctgcacc gtcacggtga tggcccgatc gatgcgttca cggacgctgg tgtgggagat
      241 atcggcgccg gcgacacgaa ccgagcggcg ccattcgccc atcgccgcgg cgaacacggc
      301 ggccattgga tggttggggt tgctgccctc gctgtcgaac aactcgtcca ggctcccgcc
      361 ggaccagaag cggtcctcga agccatcggc ggccttgttg accttgcgta gagtgctcca
      421 tttttcgaag accaccgccc atacccacac gctggcgccc agcaagccga tcattaccag
      481 cttcacgacg atgtccgctt gcacgaacag accccacagc gagaggtcgc cgttcgccgt
      541 ccccaggttg accgcatcca ctacccgatc cactgcctaa tccccttgct gcgcgcctgc
      601 accctgatca tgatattttc ccgcgcccgg cgttgcttta cccggcaaag ctgccatcgc
      661 ctcacgccac ggcggcggga tgcgggccgg tttgtttccc ccgattcgga cgcaggccag
      721 cttgatgcgc agcgtcgcgc aaaccccgtt ggggccgcga accgtctggc gcaacgatac
      781 ggttgctccg cctacatcca tcgtttccgt ctcgaccacc agatggtcgt cgagcatcgc
      841 ggggcgcaca taatctattt cggcgcgatg caccatgaac atcaggcgga atctttcaac
      901 gagatccgca tgcgggattc ccgcgtcgcg caaagcctcg gttcgcgcgc gttcggcgta
      961 acggaggtag ttggcgtgat agaccacacc gccggcatcg gtatcctcat agtaaacccg
     1021 caagggatag tggtgcaggg atcgcggccc agtggtcatc taaatccgcc cggagcagta
     1081 ccgcgcacgg aatcgcggtg ataccgctgg ccgccaatcc ggagcgaggg gccgtggcgg
     1141 cactattcct ctccccccag caagtcgagc aaaggcgcgc cggagcgtgg cggcttcagc
     1201 ccaagatgcc gccagcccgg ctcgcccaac atgcggcctc ggctggtgcg caggatcagg
     1261 ccttcctgga tcaggtatgg ctcgatgacg tcctcgagcg tgtcgcgcgc ttcggccagc
     1321 gcggcggcga gggtctcgac gccgaccggg ccgccggcgt ggtggtcggc gatccgtcgc
     1381 aggtagcgcc ggtccatgga atccaggcca agccggtcaa cctccagccg gcccaaggcg
     1441 gcgtcggcaa tggctcggtc aaccggcgtg tcaccctgta cggcggcgaa gtcgcggacg
     1501 cggcggagca ggcggccggc gatgcggggg gtcccgcggg agcgccccgc gatttccgcc
     1561 gcaccttccc cggtcaggct caggcccagc agcctggcgc cgcgaacaat gatctgacgc
     1621 aactcttccg gcgtgtagaa caccaggcgc agcgggattc cgaagcggtc gcgcaacggt
     1681 gtcgccagca ggccggcccg cgtcgtggcg ccgaccaggg taaacggtgg caggtcgatg
     1741 cgcacgctgc gtgccgccgg cccctcgccg ataatcaggt cgagctggaa gtcttccatc
     1801 gcgggataca ggatttcctc gatcgccggc tgcaagcgat ggatttcgtc gataaacaag
     1861 aca
//
LOCUS       DASWQN010000897         4881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2065623, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000897 DASWQN010000000
VERSION     DASWQN010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065623"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VGC82_15240"
     CDS             complement(<1..809)
                     /locus_tag="VGC82_15240"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEY0424672.1"
                     /translation="MMSAVTPPAATLPDAVRAGADLKAARERLGLSLEEVARDLRIRL
                     PHLKALEEGRVSELPGNAFAWAFVRSYAGSLGLDVEAMVRRFRTGTTRPDSRMELDFP
                     VPVAERGLPAGAMALLGVVLAVGAYAGWYRLSGEGRLPAEAVTAIPQRLASLAEQALP
                     PSSGPPNAIADATPPVMPDLPADLGNLADPAPPVMAISPTSAAAAQLPPRAEDPPAAA
                     GLAPPVDATRIVLRAIADSWVMVKDRSGTILLNRILRIGETWPVPGRKDLVL"
     gene            complement(1046..3268)
                     /locus_tag="VGC82_15245"
     CDS             complement(1046..3268)
                     /locus_tag="VGC82_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEY0424673.1"
                     /translation="MTAPRRLLSRLRNLRAHGSAPLSALVQLVAAEMVSEVCSIYVQR
                     PGDILELAATEGLNQVAIGRTRLRVGEGIVGLCAATGAVMNLPDAQNHPAFAYRAETG
                     EEPFASLLAVPVRRSGRTLGVLVVQNRTPRNFTEPEVDDLETVAMLLAEMLSGSGATD
                     GSPEGLAATVPRLFAGTSLMGGIAIGPIVLRRSARPIVSLLADDPEAEQLRLDQAVDR
                     MQRGLDDLFADVPSNGHTADVNASREVLEAYRLVASDAGWLRRVADVIRGGLTAEAAV
                     QRVAGELHNRMRRISDAYLRERLADMEDLANRLLAALTGEVSRAAVPEGAILLARRLG
                     PAELLDWHARGIAGVAIEEASPSGHAAILTRALGIPAIGNLRGILDSAEQGDVAVVDA
                     DEGQFILRPEAEVQRTYTRAREARDAKYAELAAWRGHPAATADGVALKLMLNVGLSME
                     LPQLERTGADGIGLFRTEIAMLARGSVADVSEQAAFYARVLDEAGDKPVLFRTLDLGA
                     DKLLPGTVSEEENPAMGWRSLRVGLDRPALLRRQLRALLLAAGGRDLSVMFPMVATVA
                     EFHAAKALLLAEAKRVRPAVGALHIGTMLEIPSLMWQLPDLLKEADFISVGSNDLLQF
                     LFAADRGTPSLYDRYDFLSPPVLDLLQQLVVAASAAKIPVSLCGEAASRPLEALVLAA
                     LGITTLSMPASRILPIKAMLSQVDLMSFRAVLTAIRRGGGSGLRESVATWAREHGILA
                     "
     gene            complement(3549..4586)
                     /locus_tag="VGC82_15250"
     CDS             complement(3549..4586)
                     /locus_tag="VGC82_15250"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY0424674.1"
                     /translation="MSSDPHIAARARLDRLWSRGTEFLGCPYAILGGAMSWVSEQHLV
                     SAISNAGGFGVIACGAMNPAQLAEQIAGTRARTDKPFGVNLITMHPELNDLIRVTLEA
                     GVGHIVLAGGIPPGPAVRAVKDSGAKLIAFSPALSLAKRLVRSGADAIVIEGSEAGGH
                     IGPVSLSVLAQEVLPHLRDVPVFVAGGLGRGEAILSYLEMGASGAQLGTRFAASEESI
                     AHVKFKKFFVHANARDAMPSVELDPRLPVIPVRGLVNDGTRKFIQHQAETRDKLLAGE
                     VTKEEAQLLVERFWAGALRRAVIDGDIETGSVMAGQSVGMVTSIQPVAEIIQELIGQA
                     VAALAARAEAG"
     gene            complement(4576..>4881)
                     /locus_tag="VGC82_15255"
     CDS             complement(4576..>4881)
                     /locus_tag="VGC82_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY0424675.1"
                     /translation="MTFTVGKADLPRARAALAGIKSQINYAELLEDPDVAKISVVGVG
                     MRSHVGVANTMFSALADKAINIQVISTSEIKVSVLIAAEYTELAIRALHTAYGLDVV"
BASE COUNT          892 a         1628 c         1597 g          764 t
ORIGIN      
        1 agcacaaggt ccttccgacc cggcaccggc cacgtctcgc cgatcctgag gatacggttg
       61 agcaaaatcg tgccggagcg gtccttcacc atcacccagg aatcggcgat ggctcgaagc
      121 acgatgcggg tcgcatccac cggcggcgcc agcccggcgg ctgccggcgg atcctccgcc
      181 cgtggcggga gttgcgcggc ggcagccgag gtgggggaga tcgccatcac cggaggggcc
      241 ggatcggcca ggttcccaag gtcggctgga agatcgggca tcaccggcgg cgttgcatcg
      301 gcaatcgcgt tgggcggacc ggacgagggt ggcaaggcct gttcagccag cgacgccagc
      361 cgctgtggaa tcgcggtcac cgcttcggcg ggaaggcgtc cctcgccgga cagacgatac
      421 cagccggcgt aggcgccgac cgccagaaca acgcccaaca gcgccatcgc accggccggc
      481 agtccgcgtt cggcgaccgg caccgggaaa tcaagttcca tgcgagagtc gggtcttgtg
      541 gttccggtcc tgaagcgccg aaccatcgcc tctacatcca ggcccaggct gccggcatag
      601 ctgcgaacaa aagcccatgc aaaggcattg cccggcaact ccgacacacg gccttcctcc
      661 agcgccttca gatgcggcag ccggatgcgc aaatcacggg cgacttcttc aagcgacagg
      721 cccaggcgtt cacgggcggc cttaagatcg gcgcctgccc gcaccgcgtc ggggagcgtc
      781 gctgccggcg gcgtgacggc tgacatcata ccccgattct cgatcagttt ggacctgcgt
      841 tcctgcagca cggcagcccc gttccaatcc ggaggttgta ggaaaatagc ttgccgaacc
      901 cggccgcggc cagtccggtt ccggtgggtt tctagaccac gcgacgcaca tttccaatgt
      961 cataaatcga tttgttttcg ttttcacagc ctgaaaatca ggatcgatat caagattatt
     1021 atctagcggc ggtgcgacgg ctggatcagg caagaatccc gtgctcacgc gcccaggttg
     1081 cgacggactc tcgaagcccg ctgccgccgc cgcggcggat cgcggtcagg accgctcgga
     1141 aggacatcag gtcaacctgc gacaacatcg ccttgatggg aaggattctg cttgccggca
     1201 tcgacaacgt ggtgatgccc aaagccgcga gcaccagtgc ctccagcggt cgggacgccg
     1261 cttcgccgca cagcgagaca ggaattttgg cggcgctggc cgcaactaca agttgctgca
     1321 gcaggtcgag aaccggaggc gagagaaaat catagcgatc gtataatgaa ggtgtgccac
     1381 ggtccgccgc gaatagaaac tgcaataaat cgttagagcc aaccgagatg aagtcggctt
     1441 ccttcagcag gtccggcagt tgccacatca acgacgggat ctccagcatg gtgccgatat
     1501 gaagcgcccc gacggctggg cgaacccgtt tggcttcggc cagcagcaac gccttcgcgg
     1561 catgaaattc cgccaccgtc gcgaccatgg ggaacatcac cgacagatca cgcccgccgg
     1621 cggccagcag cagggcacgc aactgccgtc gcagcagcgc cggacggtca agccccaccc
     1681 gcagcgaccg ccagcccatc gccgggtttt cttcctcgct gaccgttccc ggcagcagct
     1741 tgtcggcgcc caggtccagc gtgcggaaca gcaccggttt gtcgccggcc tcgtccagga
     1801 cgcgggcgta aaacgcggct tgctccgaca catcggcgac cgaaccgcgt gcgagcatgg
     1861 cgatctcggt gcgaaacagg ccgatgccgt cggcaccggt gcgctccagt tgcggcagtt
     1921 ccatgctgag gccgacgttg agcatcagtt tcagcgcgac gccgtccgcg gtggcagcgg
     1981 gatggccccg ccatgccgcg agttcggcat atttggcgtc gcgggcctcc cgggcgcggg
     2041 tataggtgcg ttggacctcg gcttccgggc gcaggatgaa ctggccttca tcggcgtcca
     2101 ccaccgcgac gtcaccctgt tcggcgctgt ccaggatgcc ccgcaaattg ccgatcgctg
     2161 gaatcccaag cgcgcgggtc aggatggcgg catgaccgga agggctcgcc tcctcgatgg
     2221 ccacaccggc gataccgcgg gcatgccagt ccagaagttc ggccggaccc agccgccgcg
     2281 cgagcaggat ggcaccttcg gggacggcgg cccgggaaac ctcaccggtc agggcggcca
     2341 gcaggcggtt cgccagatcc tccatatcgg ccaggcgctc gcgcaggtag gcgtcgctga
     2401 tacggcgcat cctgttgtgc agctcgccgg caacgcgctg caccgctgcc tcggcggtca
     2461 ggccgccgcg aatgacatcg gcgacccggc gcagccagcc agcgtccgat gcgaccagcc
     2521 gataggcttc cagcacttcg cgggacgcat tcacgtcggc ggtgtgtccg ttgctgggaa
     2581 catccgcgaa caggtcgtcc aatccccgct gcatgcgatc caccgcctgg tcgagccgaa
     2641 gctgttcggc ttcgggatca tcggccagca ggctgacgat gggacgggcg gaacggcgca
     2701 agacaatcgg gccgatggca atgccgccca tcagcgaggt gccggcgaac aatcgtggca
     2761 ccgtcgcggc cagcccctcg ggcgagccat ccgtggcgcc gctgcccgac agcatctcgg
     2821 ccagcagcat ggcgacggtt tcgaggtcat cgacctccgg ttcggtgaag ttgcgcgggg
     2881 tgcggttctg caccaccagc acgccgaggg tgcggccgga ccggcgcacc ggcaccgcga
     2941 gcaaggaggc gaagggttcc tcgccggttt ccgcgcggta agcgaaggcc gggtggtttt
     3001 gggcgtccgg caggttcatc accgcgccgg tggccgcgca caggccgacg ataccctcgc
     3061 cgacccgaag ccgggtgcgg ccgatagcga cctgattgag cccctccgtg gccgcgagtt
     3121 ccagaatgtc gccgggccgc tggacataga tcgagcacac ctcgctgacc atttcggccg
     3181 caaccaactg gaccagcgcg gacagcggcg cggagccatg ggcgcgcagg ttccgcaacc
     3241 tggaaagcag gcgcctaggc gcggtcattc acttcaggcg aaatcgatat atttgccatc
     3301 ctcgagaccg cctccggttt gccgcggttg gccccaagcg gccggccaat acgggcggcc
     3361 aacacatggg cggcatggct gagcatggca gcgaggcggc cgcggagcaa ggaaagtcgg
     3421 gccggcgagg caccgcgaag ccggcgggga gtgcctggtg ccgcagagaa tcgatccagt
     3481 gcgggcggtt gcgaatggat gcgacgaaac agggggataa cttccagcct gtccggcttg
     3541 gcaatgatct agccggcttc agcccgtgcc gcgagcgccg cgaccgcctg gccgatcagt
     3601 tcctggatga tctcggcaac cggttggatg cttgtcacca tccccacgga ctggccggcc
     3661 atcaccgatc ccgtttcgat gtcaccgtcg atcaccgcgc ggcgcaacgc gcctgcccaa
     3721 aagcgctcga ccaaaagctg ggcttcttcc ttcgtcacct cgccggcgag aagtttgtcc
     3781 cgcgtttcag cctggtgctg gatgaacttc ctggtgccgt cgttgaccag tccgcgcacc
     3841 gggatcaccg gcagacgcgg gtcgagttcg accgacggca tggcgtcgcg cgcattggca
     3901 tggacgaaaa acttcttgaa cttgacatgg gcgatcgatt cctcggacgc cgcgaagcga
     3961 gtgccgagct gcgctccgga tgcgcccatc tccaggtagg aaaggatggc ctcgccacgc
     4021 cccaggccgc cggcgacgaa caccggcaca tcccgcaaat gcggcaggac ttcctgcgcc
     4081 aggaccgaca gcgacaccgg cccaatatgg ccgccggctt ccgaaccctc gatgacgatg
     4141 gcatcggcgc cggagcggac aaggcgtttc gccagcgaca atgccggaga aaacgcgatc
     4201 agcttggcgc cactgtcctt gaccgcgcga accgcggggc ccggtgggat accgccagcc
     4261 aggacgatat gcccgacccc ggcctccagc gtcacccgga tcaggtcgtt cagctccgga
     4321 tgcatcgtga tcaagttcac gccgaacggc ttgtcagtcc gtgcccgtgt gccggcgatc
     4381 tgctcggcca actgcgcggg gttcatcgcg ccgcaagcga tgacgccgaa gccgccggcg
     4441 ttcgagatgg cggaaaccag atgctgctcg ctgacccagc tcatggcgcc gccaaggatc
     4501 gcatacgggc aaccgagaaa ttcggtgccg cgcgaccaca accggtcaag ccgagcgcga
     4561 gcggcgatat gggggtcaga cgacatcgag gccgtaggcg gtgtgcaggg cgcggatagc
     4621 cagttcggtg tattccgcgg cgatcagcac gctgaccttg atctcactgg tggagatcac
     4681 ctggatgttg atcgccttgt ccgccagcgc gctgaacatg gtgttggcga caccgacgtg
     4741 gctgcgcatc cccaccccaa cgacgctgat cttggcgaca tccgggtctt ccagcagttc
     4801 ggcgtagttg atctggctct tgatccccgc cagcgcggca cgcgcccgcg gcagatcggc
     4861 cttgcccacg gtgaaggtca t
//
LOCUS       DASWQN010000898         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1801528, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000898 DASWQN010000000
VERSION     DASWQN010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801528"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..700
                     /locus_tag="VGC82_15260"
     CDS             164..700
                     /locus_tag="VGC82_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0424676.1"
                     /translation="MFTRSPSAVMFLLITVALCRPAPANAETPNLGRPLSPQDLAPWD
                     ISILPDGTGMPPGSGTAAQGEALYAAACQGCHGERGAGKPNDPLAGGQGTLAKPAPVK
                     TVGSFWPYATTLFDYVRRAMPLNAPKSLTDDQVYAVCAYILQVNGIIGANETMDARSL
                     PAVRMPNRDGFITFVRNH"
     gene            963..>2454
                     /locus_tag="VGC82_15265"
     CDS             963..>2454
                     /locus_tag="VGC82_15265"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0424677.1"
                     /translation="MKSPVGKDWVTNGGNLTNQRYSTLKQINTENAGQLKGAWMTRLK
                     GSGLGGKYSAEATPLIRNGTMYVVTGNDDVFALNAKTGEILWERWSHIDQAINTVCCG
                     WLNRGLAMGEGLLFLGQLDTNVVALDIKTGKEVWKTPIEEWKNGYVVTSAPLYYDGIV
                     YSGIAGGEFGTRGRLTALDAKTGKILWRSYTLPAPGEVGSDSWPKGSTQAMRGGAPIW
                     NTPAVDPELGLLYFATGNCGPDYDGASREGDNLFCASIMAINAKTGQYAWHFQEVHHD
                     IWDYDAASPVVLFDTVINGQPRKGIAEAGRTGWVYILDRTNGKPLIGIEEKPVPQEPR
                     QHTAATQPYPIGDNTVPTCAPPLPGYEKPGCIFDAFWETPVLIQPGGQGGTNWSPMPY
                     SPDTGFFYVPGTIRSSTFGRLGDSYKNGQRYTGGTQSAPIGSQFGGTFTAIDSHSNKI
                     AWQQQMPYRMGGGGGSTVTAGGLLLRGEPDGNFVVLNAKSGEVLWKF"
BASE COUNT          505 a          736 c          779 g          434 t
ORIGIN      
        1 cagtgggacg gcggcccggc ggtacttggc agccgagccg tcgatgagtc cggatatgtg
       61 cagccaagcc gggatgcgtt gctggctgaa cgaggtggca acacgatcta ccactacaac
      121 ggcatcacct gctggggcgt cgcgacgagc ggcgagttaa gtcatgttta cgcgtagccc
      181 atcggcggtc atgttcctgc tgattaccgt ggcattgtgc aggcccgctc cggcgaatgc
      241 cgagacaccc aatctcggcc ggccactttc gccacaggac ctggcaccct gggacatcag
      301 catcctgccc gatggcaccg gaatgccgcc tggcagcggc acggcggcgc aaggcgaagc
      361 gctttacgct gccgcgtgcc agggctgtca cggcgaacgg ggtgccggca agccaaacga
      421 cccactggct ggcggccagg gaaccctcgc caagcccgcg ccggtcaaga ccgtgggcag
      481 tttctggccg tatgccacga cgctgttcga ctatgtcagg cgtgccatgc cgttgaatgc
      541 accaaaatcg ctgaccgacg atcaggtcta tgcggtgtgt gcttatatcc tgcaggtcaa
      601 cggtatcatc ggcgcgaacg agacgatgga tgcgcgcagc ctgccggcgg tacgaatgcc
      661 gaatcgcgac ggtttcatta ctttcgtccg aaaccattag cccagatcga ggctgatccg
      721 aggctgttgc aatcgccatc ggcggcggcg cacttgccag gaatgcaaca accaagcatc
      781 ctgttggcgt ggtccggttc cttccggaga gtgtcgagca gacaatgtca ataggctgga
      841 gggggagaaa agaaatgtca attcgcggtg tccgattatt ggttggagcc gtggcgatct
      901 gcctgctttg cctggccgac gcaccacccg ttcgggctgc ggacccggaa atagaccagc
      961 tgttgaagag tcccgtcggc aaggactggg taaccaacgg cgggaacctg accaaccagc
     1021 gctattcgac tctgaagcag atcaacaccg agaatgcggg ccagctaaaa ggggcctgga
     1081 tgacgcggct caaaggctcc ggcttgggcg gcaagtattc ggccgaagcc acgccgctga
     1141 tccgcaatgg caccatgtat gtggttaccg gcaatgacga tgtcttcgca ctgaacgcga
     1201 aaaccggcga aatactctgg gagcgctggt cgcacattga ccaggcgatc aacacggttt
     1261 gctgcggctg gctcaatcgc ggcctcgcaa tgggtgaggg gctgttgttc ctaggccaac
     1321 tcgacaccaa cgtcgtggcg ctcgacatca agactggcaa ggaggtgtgg aagacgccga
     1381 tcgaggagtg gaaaaacggt tacgtcgtca ccagcgcacc gctgtactac gacggcatcg
     1441 tctacagcgg cattgccgga ggcgagttcg gcacccgcgg gcgcttgacg gcgcttgatg
     1501 cgaagaccgg aaagatcctg tggcggtctt acactctgcc ggcaccgggc gaagtcggaa
     1561 gtgatagctg gccgaaaggc agcacccagg cgatgcgcgg cggtgcgccc atctggaata
     1621 ctcccgcggt cgatccggag cttggcctgc tttacttcgc gacgggcaac tgcggaccgg
     1681 actatgacgg tgcctcacgt gagggcgaca atttgttctg tgcctcgatc atggcgatca
     1741 atgccaagac cggccagtac gcctggcatt ttcaggaagt gcaccacgac atctgggact
     1801 acgacgcggc aagcccggtg gtgctgttcg acacggtcat caacggccag ccgcgcaagg
     1861 gcattgccga agccgggcgc acaggctggg tctatatcct ggaccgcacc aacggcaaac
     1921 cgctgattgg gattgaagaa aaacccgtgc ctcaggaacc gcggcagcat accgcggcga
     1981 cccagccgta cccgatcggc gacaacacgg tgccgacctg cgcgccgccc ctgcctggct
     2041 atgaaaaacc gggatgtatc ttcgatgcgt tctgggaaac accggttctg attcaacccg
     2101 gtggccaggg cggcaccaac tggtcaccga tgccatacag ccccgatacc ggtttcttct
     2161 atgtgccggg caccattcga tcgagtacat tcgggcgatt gggcgactcc tataagaacg
     2221 gtcaacgcta taccggtggc acccagtccg cgccgatcgg ctcgcagttc ggaggtactt
     2281 tcaccgcgat cgacagccac tccaacaaga tcgcctggca gcaacagatg ccctaccgca
     2341 tgggcggcgg cggcggctcg acggtgaccg ccggcggtct gctgctgcgt ggcgagccgg
     2401 atggaaactt cgtcgtgctg aatgcgaaat cgggcgaggt gctatggaag ttcc
//
LOCUS       DASWQN010000899         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_878134, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000899 DASWQN010000000
VERSION     DASWQN010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878134"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VGC82_15270"
     CDS             <1..417
                     /locus_tag="VGC82_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424678.1"
                     /translation="QHRGGPVGFVRRLDERTIGWADYTGNRQYVSVGNAAADDRVALI
                     FVDYPHRERLKVLGHMRTFDVDARPDLTPRLVVQHHKARVDRFILITVEAFDWNCPQH
                     ITPRFTLAEIEKLTAPLYARIAELEAQAKQQGTFLG"
     gene            634..1455
                     /locus_tag="VGC82_15275"
     CDS             634..1455
                     /locus_tag="VGC82_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0424679.1"
                     /translation="MNTITVKDGTQIYFKDWGRGQPVVFSHGWPLSADAFEDQMFFLA
                     SQGYRCIAHDRRGHGRSSQPWNGNDLDTYADDLAALVQALDLRNAVHVGHSTGGGEVA
                     RYIGRHGTERVAKAALIGAIPPLMLKTADNASGTPIDVFDQLRSGVQADRSQFWKELS
                     LPFYGYNRPGAKISEGVRESFWLQSMMAGFPASYFCIKAFSETDLTEDLARFDKPTLV
                     LHGDDDQIVPIGASAMRSSKLIKNATLKIYKGAPHGMCTTLKDQVNAELLAFLTE"
     gene            1526..2497
                     /locus_tag="VGC82_15280"
     CDS             1526..2497
                     /locus_tag="VGC82_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0424680.1"
                     /translation="MTRARQHSLSRRGFCFCCVSASAFAARGGWVTPGQAFAEANGIV
                     QLIKSEAAKAPITVHRVRGNVTILEGSGGNIAVLAGRDGKVLVDAGIAVSRPQVATAL
                     AGIGPEPVTHLINTHWHFDHANGNEWLYELGPRIVAHENTRKHLSVSQRVDDWSFDFP
                     ALSAGALPTDLFTTERTFKLNDCTLELKYYGPAHTDSDISVRFAESDILHTGDTFWNG
                     IYPFIDYSTGGSIDGSIRAADANLTATTDATIIIPGHGQPVSNRAQLQEFRDMLVGIR
                     ENVATLKKQGRPLEDIVKAKPTAAYDAKWGKFVIDPPLFTKLVYEGV"
BASE COUNT          555 a          780 c          753 g          526 t
ORIGIN      
        1 cagcaccgcg gcggcccggt cgggttcgtt cgccgcctgg acgagcgaac catcggttgg
       61 gccgattata ctggcaaccg ccaatacgtc tcggtcggca acgcggccgc ggatgatcgc
      121 gtagcattga tcttcgtcga ttatccccat cgagaacggc tgaaagtgct cggtcatatg
      181 cggacctttg acgtcgatgc tcggcccgat ctgacacccc ggctggttgt ccaacaccac
      241 aaggcccgag tggaccgctt tatcctgatt acggtggaag catttgactg gaattgcccc
      301 cagcacatca caccgcgctt taccctggcc gagatcgaaa agctgacggc accactttat
      361 gccagaattg ctgagcttga ggcgcaagca aagcagcagg ggacttttct cggctgagtt
      421 ttttgcagac gcgctcggag acagcgatcg tcggctacta tgcggaacta gaagctgaaa
      481 ccgggcccca ggttttgcca gtccactcta agtccaaaaa gcttcggccg agcgccaaca
      541 acaggacaag tcgacaccct tccagagcag catccgtttg tctgtctctg cgataccggc
      601 cgttctctga acctgcaaag gggatataac aacatgaata caatcacggt gaaagacgga
      661 acacagatct actttaaaga ttggggcagg ggacaacctg tcgtctttag ccatggctgg
      721 ccgctcagtg ctgatgcctt cgaagaccag atgttcttcc tggcgtcgca aggctatcgc
      781 tgcatcgcgc acgaccggcg cgggcacggc cggtccagcc agccctggaa cggcaacgat
      841 ctggatacct atgccgacga cctcgccgcc ttggtccagg cactcgattt gagaaacgcc
      901 gtccatgtcg gccattcaac gggcggcggg gaggtcgccc gctacatcgg ccgtcacggc
      961 accgagcgcg tggcgaaggc ggctctgatc ggtgccatcc cgccgctgat gctgaagaca
     1021 gcggacaatg ccagcggaac ccccatcgac gtgttcgatc agctccgctc tggcgtccag
     1081 gctgaccgtt cgcagttctg gaaggaactc agtctgccgt tctacggcta caaccggcct
     1141 ggcgcgaaga tttccgaagg tgtacgcgag tcgttctggc tgcagagcat gatggctggg
     1201 ttccccgcgt cgtacttctg catcaaggca ttttccgaaa ccgatctgac cgaggacctc
     1261 gccaggtttg ataaaccaac tttggtcctg cacggcgacg acgaccagat cgtacctatc
     1321 ggcgcgtcgg cgatgcgctc gtccaaactg atcaaaaatg ccacgctcaa gatctacaaa
     1381 ggcgcaccgc acggaatgtg cacaacgctg aaggatcagg tcaacgcgga attgctggca
     1441 tttctgacgg agtagctacc agccgctcaa gttgcgcgat gccgtaactt gagcggccat
     1501 gatttgtagt ccgcaggagg aaccgatgac ccgtgcacgt caacactccc tctcacgtcg
     1561 tggcttttgc ttttgctgcg tctcggcatc ggccttcgca gcgaggggcg gatgggtcac
     1621 gccaggtcag gcttttgccg aggcaaacgg cattgtccaa ctgatcaagt ccgaagccgc
     1681 caaggccccg atcaccgtcc accgagtccg aggcaacgtc accatacttg agggatctgg
     1741 cggcaacatc gcggttctgg ccgggcgtga cgggaaggtt ttagtagatg ctggcatcgc
     1801 cgtttcccgt ccccaggttg ccaccgccct tgcgggcatc ggccccgaac cggtcaccca
     1861 tctgatcaac acccattggc acttcgacca tgccaatggc aatgagtggc tgtatgagct
     1921 tggtcctaga atcgtggcgc acgagaatac gcgcaagcac ctttcagtaa gccagcgcgt
     1981 ggacgactgg tctttcgact ttccggcctt gtctgccggc gcgctaccga ccgacctgtt
     2041 cacgacggaa cggacattca agctgaacga ttgtacactg gagctgaagt attatggtcc
     2101 ggctcatacg gacagcgaca tctccgtccg cttcgccgag tccgatatcc tgcacaccgg
     2161 cgacacgttc tggaacggca tatacccctt catcgactat tcgacgggcg gcagcatcga
     2221 cggctccatc cgagcagcag acgccaatct gacggcgacg accgatgcga ccatcatcat
     2281 tcccggacat ggccagccag tcagcaatcg agcgcagctc caggagttcc gggatatgct
     2341 cgtgggtatc cgcgagaacg tcgcgacgct gaagaagcag ggcaggccgt tggaggacat
     2401 cgttaaggcg aagcccaccg ctgcttatga tgcgaagtgg ggtaaattcg taatcgaccc
     2461 gcctttgttc accaagctgg tttacgaggg cgtctgagtc cttcgatccg ggggaatcca
     2521 ggaaagcctg attcgccgat tgccgggtag tattggtagc cggagtgact gtttattggt
     2581 gacggtaagt gcaatatcgg gggatttcga tgaa
//
LOCUS       DASWQN010000900         3992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_350543, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000900 DASWQN010000000
VERSION     DASWQN010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350543"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VGC82_15285"
     CDS             <1..402
                     /locus_tag="VGC82_15285"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY0424681.1"
                     /translation="LAADPVEEIKVGFDILKSMGLRHRGVNIIACPSCARQGFNVIAT
                     VATLEERLAHIKTPITLSIIGCVVNGPGEALMTDIGVTGGGNGRHMIYAAGKTDHTID
                     SASMVDHIVELVEAKAASLKAEMEREKIAAE"
     gene            661..1041
                     /locus_tag="VGC82_15290"
     CDS             661..1041
                     /locus_tag="VGC82_15290"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0424682.1"
                     /translation="MSTNMADSNAAAREAEELEDQLDAALLKLARVEQANEETVPLEL
                     VRRLSDGDVPVTVWREHRGLSQEELSVAAQVQPEMLKAVESGKEGVPLRIMHAIARAL
                     GVDLDDLVPWSTESDVSGYRTHAQ"
     gene            1428..2687
                     /gene="hisS"
                     /locus_tag="VGC82_15295"
     CDS             1428..2687
                     /gene="hisS"
                     /locus_tag="VGC82_15295"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY0424683.1"
                     /translation="MASWRRLQRNNLQPARGTHDFIGEEQRRFHHVVDIARRVSATYG
                     FDEWQTPIFEDTSVFARTLGETSDVVTKEMYTFDDRGGDSVTLRPEGTAGVCRALVTN
                     GLTQSLPQKIFYAGPMFRYERPQKGRYRQFHQIGIELIGPAEPLADAEVIACGWDILQ
                     ALNVTDDTVLELNTLGDKESRDAYRTALVAYFTDHKTALSHDSLTRLERNPMRILDSK
                     DEADRRIVADAPTIAPYLTPAAGAFYDKLQEHLVRFRVPFRKNPRIVRGLDYYGHTAF
                     EFVTAKLGAQGTVMAGGRYDGLVAEMGGPATPAVGWAAGIERLAMLMQNAPPTPSPVA
                     VIPVGDAAEATAIEILQSLRANGIRAEMAYRGNLKRRMERANRTGARAAVILGDDDIA
                     NGVAQVKDLATGSQEAIPLSQIPARLR"
     gene            2684..3739
                     /gene="prfA"
                     /locus_tag="VGC82_15300"
     CDS             2684..3739
                     /gene="prfA"
                     /locus_tag="VGC82_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY0424684.1"
                     /translation="MSLAFKLDRIAARAEELRAMLSEGISGEAYVKASKELSDIEPVV
                     ARIGDLRTAERAKSDAESMLTDPEMKDLAEAELYDLKEQIPALEHEIRLALLPKDEAD
                     ARSAILEIRPAAGGDEAGLFAAELFAMYQKYADLKGWRFEILEYGDTGLGGIKEATAE
                     ITGRNVFARLKYESGVHRVQRVPATESQGRIHTSTVTVAVLPEAEEVDVQINEDDLKI
                     DVYRASGAGGQHVNKTESAVRITHIPSGIIVAMQEERSQHKNRAKAMKILRARMYEQQ
                     RSSLAATRSADRKSQVGTGDRSERIRTYNFPQGRVSDHRINLTLYKIDRVMQGDLDDF
                     IDALTAEDQAARLAAEE"
     gene            3784..>3992
                     /locus_tag="VGC82_15305"
     CDS             3784..>3992
                     /locus_tag="VGC82_15305"
                     /inference="COORDINATES: protein motif:HMM:NF037644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424685.1"
                     /translation="MAAASAINKMPTAVLVTITEAVRAGAASLDGIADNPRLEARLLL
                     AHTLSLTRNDLIRDPERRIETTDFEA"
BASE COUNT          826 a         1252 c         1241 g          673 t
ORIGIN      
        1 ctcgccgccg atccggtcga ggagatcaag gtcggcttcg atatcctgaa gtccatgggc
       61 ctgcgccatc gcggcgtgaa catcatcgcc tgcccgtcct gcgcgcggca gggcttcaac
      121 gtgatcgcga cggtcgcgac gcttgaggag cggctggcgc acatcaagac gccgatcacc
      181 ctctcgatca tcggctgcgt ggtgaacggc cccggcgagg cgctgatgac cgacatcggc
      241 gtgaccggcg gcggcaacgg gcggcacatg atctatgccg ccggcaagac cgaccacacg
      301 atcgacagcg cgtccatggt ggatcacatc gtggaactgg tcgaggcgaa ggcggcgtcg
      361 ctgaaggcgg aaatggagcg ggagaagatc gccgcggagt gacgtatggc gctcaagatt
      421 ttcttctatc cacgagcgcg gaaagaactc cagggtcttc cgaaggctga tcgcgacaga
      481 gtgctgcagc gccttcaggc ctacgcggcg gatccggaca atcctcatca cgcggtcgtc
      541 gcactggttg gggaaaagcc gatccgtaag gctgcgtgtg ggggattggc gtgtattatt
      601 cgaccgcatt gacgataaga tagaggtccg tggggtccgg caccgccggg aggcatatcg
      661 atgagcacaa acatggcaga tagtaacgct gctgcacgcg aggcggagga acttgaggac
      721 cagctcgacg ccgccctgtt gaaactggca cgggtggagc aggctaacga agagaccgta
      781 cctttggagc tcgttcggcg cctatccgac ggggatgttc cggtcactgt ctggcgtgag
      841 caccgaggtt tgtcccagga ggaactctcg gtcgccgctc aggtccagcc agaaatgctg
      901 aaggccgttg agagtggcaa agagggcgtg ccgctgcgga ttatgcacgc gattgcccgc
      961 gcgctcggcg ttgatcttga tgatctagta ccttggagca cggaaagcga tgtgtcaggg
     1021 tacaggaccc atgcgcaatg actctatcgt ctgttcgctt tcctgattgt gccgtaagcg
     1081 acccagccga tcaaccgtag aagaatgggc tgacgtgcgc aggtcatttg cttcgcgggc
     1141 caacagacgg catagggttc tgaaatcgcc cacggactca ccccgtcatc tacggaatca
     1201 aacttgaaat gcaggtaaag ttatcgaccg gtgaggtcat tgaacttctt ggtccagtcg
     1261 tgagccgcag acctgatgga accacggaga ctagtcgtcg gaacttccgc gcatccatag
     1321 gtaccgaaga ggtgcagatt tcgattgagg ccttagacgg ggcacaattt cccgaagacg
     1381 ttactaacaa ggctgagaca gaattcgcga agtcgctgcc ggacgtgatt gcaagttggc
     1441 gccgcctcca gcggaataat cttcaacccg cccgcggcac gcacgacttc atcggcgagg
     1501 aacagcgccg tttccaccac gtcgtggaca tcgcgcggcg tgtctcggcc acgtatggct
     1561 tcgacgagtg gcaaaccccg atcttcgaag acaccagcgt cttcgcccgc accctgggcg
     1621 agacctccga cgtcgtcacc aaggaaatgt acacgttcga cgaccgcggc ggcgattccg
     1681 tcaccctgag acccgaaggc accgccggcg tctgccgagc gctcgtgacc aacgggctga
     1741 cgcagtcgtt gccgcagaaa atcttctacg ccgggccgat gttccgctac gaacggccgc
     1801 agaaaggccg ctaccgccag ttccaccaga tcggtatcga gctgatcggt cctgccgaac
     1861 ctttggcgga tgccgaggtg atcgcctgcg gctgggacat cctgcaagcg ctgaatgtca
     1921 ccgacgacac ggtgctggag ctgaataccc tcggcgacaa ggaaagccgt gacgcctacc
     1981 gaaccgcact ggttgcctac ttcaccgacc acaagaccgc cttgtcgcac gacagcctga
     2041 cacggctgga gcgcaatccg atgcgcatcc tggacagcaa ggacgaagcc gaccgccgca
     2101 tcgtcgcgga tgctccgacc atcgcgccgt acctgacccc agccgccggt gccttctatg
     2161 acaagctgca ggaacacctg gtccgcttcc gcgtcccgtt ccggaagaac ccgcgcattg
     2221 tccgcggcct cgactactac ggccacaccg cattcgagtt cgtcaccgcc aagctcggtg
     2281 ctcagggcac cgtcatggcg ggcgggcgct acgacggttt ggtcgccgaa atgggcggtc
     2341 cggctacccc ggccgttggc tgggccgctg gaatcgagcg cctcgccatg ctgatgcaga
     2401 acgcgccgcc taccccgtcg cccgttgccg tcatcccagt cggcgatgcg gccgaggcca
     2461 ctgcgatcga aatcctgcaa tcgctccgcg ccaacggaat ccgagcagaa atggcctacc
     2521 gcggcaattt gaaacgtcgg atggagcggg ccaaccggac cggcgctcgc gctgccgtca
     2581 tactgggtga cgacgatatc gcgaatggcg tcgcccaggt caaagacctg gccaccggtt
     2641 cgcaggaggc cattccgctg tctcaaattc cggcccgcct gcgatgagcc tcgcgttcaa
     2701 actcgaccgt atcgccgccc gcgctgagga actgcgcgcg atgctgtccg aggggatttc
     2761 cggcgaggcc tatgtcaaag cctccaagga attgtcggac atcgagccgg tggtggcgcg
     2821 catcggcgac ctgcggaccg ccgaacgggc gaagtcggac gctgaatcca tgctgaccga
     2881 tcccgagatg aaagacctcg ccgaggccga actgtatgac ctgaaggagc agatcccggc
     2941 cctggagcat gaaattcgcc tggctttgct gcccaaggac gaggccgacg cccgctccgc
     3001 gattttggag atccgtcccg ccgcgggcgg tgacgaagca ggcctgttcg cagctgaact
     3061 gttcgcgatg tatcagaaat acgccgacct caaaggctgg cggttcgaga ttctggagta
     3121 cggcgatacc ggtctcggcg gcatcaagga agccaccgcc gaaatcaccg gccgcaacgt
     3181 gtttgcccgg ctgaaatacg agtccggcgt tcaccgcgtc cagcgtgtgc cggcgaccga
     3241 aagccagggg cgcatccata cgtctacggt caccgtcgcc gttctgccgg aagccgagga
     3301 agtggacgtc caaatcaacg aggacgacct gaagatcgac gtttatcgcg cgtcgggcgc
     3361 cggtggccag cacgtcaaca agaccgaaag cgcggttcgc atcacccata ttcccagcgg
     3421 catcatcgtt gcgatgcagg aagaaagaag ccagcacaag aaccgtgcga aagccatgaa
     3481 gatcctgcgg gcccgaatgt acgagcagca gcggtcgagc ctggcagcca cgcgctccgc
     3541 cgaccgcaaa tcgcaggtcg gcaccggcga ccgcagcgaa cgcatccgca cctataactt
     3601 cccgcaggga cgggtatcgg accaccgcat caacctgacg ctctacaaga tcgaccgcgt
     3661 gatgcagggc gacctcgacg acttcatcga cgcgctgacc gccgaagatc aggctgcccg
     3721 gctggcggcg gaggaatgat accagcgtcc cgaagggttc actgatatgc gcgcagggtt
     3781 ggtatggcgg ctgcaagcgc catcaataag atgccgacag cggtgctggt gacaattacc
     3841 gaggcggtcc gagccggtgc cgccagcctg gatggcatcg ccgataatcc tcggctggag
     3901 gcccgtcttc ttctggccca cacactcagc ctcacccgga acgatttgat ccgcgatccg
     3961 gagcgccgga tcgaaaccac cgacttcgag gc
//
LOCUS       DASWQN010000901         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_218287, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000901 DASWQN010000000
VERSION     DASWQN010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218287"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VGC82_15310"
     CDS             complement(<1..490)
                     /locus_tag="VGC82_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424686.1"
                     /translation="MTNAIDTDPRAASTAWLDAFAAALSRGDAGAAAVLFLPDGHWRD
                     LVAFTWHILTMNGRTEIAAKLDRTLPEVRPSGFRVAPNRVAPRLVTRAGTPTIEALIE
                     FETATGRGNGVLRLVPDAEGKLVAWVLLTALDEIKDHEERIGARASTGQAFSREFGGP
                     NWL"
     gene            complement(523..1278)
                     /gene="cysA"
                     /locus_tag="VGC82_15315"
     CDS             complement(523..1278)
                     /gene="cysA"
                     /locus_tag="VGC82_15315"
                     /EC_number="7.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HEY0424687.1"
                     /translation="MTIELLGLRKSFGGVAALNDIALSIADGEFLALLGPSGSGKTTL
                     LRTLAGLEFVDSGDILFNGRSMAGVPARERGVGFVFQHYALFRHMTVARNVAFGLEIM
                     PRRRRPSDREIRARVQALLDLMEIGDLGGRYPEQISGGQRQRVALARALAIEPALLLL
                     DEPFGALDAQVRKTLRVWLRQLHDRMGVTSVFVTHDQAEAMEMADRVAVMHAGRLVQV
                     DTPELLYAKPVNRFVREFLGGGQPALSSYTAQL"
     gene            complement(1275..2123)
                     /gene="cysW"
                     /locus_tag="VGC82_15320"
     CDS             complement(1275..2123)
                     /gene="cysW"
                     /locus_tag="VGC82_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEY0424688.1"
                     /translation="MSATVFPNAVRESRTVRLGLTVATVLLLAMFLLLPLAVVFTEAL
                     SRGWTAVGQALAEPDTVAAIELTLLVAAISMPVNTVCGLAAAWCIGRFQFPGRSLLIT
                     LIELPFSVSPVISGVVWVLLFGANGWFGPLLNRAGLHVIFAVPGLILATVLVTFPFVA
                     RTLIPLMQQQGSAEEEAAITLGAGFWQMFWRVTLPNVRWGLLYGVLLGNARAMGEFGA
                     VAVVSGHVRGQTMTMPLQVEALFNDYDWVGAFTIAGLLSLLALVTLAVKAVLEWHIGE
                     RTGALL"
     gene            complement(2120..2959)
                     /gene="cysT"
                     /locus_tag="VGC82_15325"
     CDS             complement(2120..2959)
                     /gene="cysT"
                     /locus_tag="VGC82_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEY0424689.1"
                     /translation="MPSGKTTGARRSALPGFAPALTITLAWVSMIVLLPLVSLALRPW
                     ELGAAGVWHAVSEPRVLAALRLSFGAAALAAAVNVPFGLLVAWVMVRYPFPGRRLADS
                     LIDMPLALPTAVAGISLTALFSPKGWIGAWLLPLGIKVAYTPLGILVALIFVGLPFVV
                     RTLQPVLIDLPLNAEEAAATLGATRSQTLRRVVLPALAPALLAGFAAALARGVGEYGS
                     VIFIAGNMPMISEIAPLLIVIRLEQLDYAGAAALGLVMLLVSFTILMLLNILQRRMGL
                     RTR"
BASE COUNT          613 a         1102 c          895 g          450 t
ORIGIN      
        1 ccagccagtt cgggccgccg aactcgcggg aaaaagcctg cccggtggaa gctcgggcac
       61 cgatccgttc ctcgtggtcc ttgatttcgt ccaacgccgt caacagcacc caggcgacca
      121 gttttccttc cgcatcgggc accagccgca acactccatt tccgcggccg gtcgccgttt
      181 caaactcgat cagcgcctcg atcgtcggcg ttcccgctcg agtcaccagc ctgggcgcca
      241 cccggttcgg agcgacgcgg aacccgcttg gccgtacctc gggcaacgtc cgatccagtt
      301 tggcggcaat ctctgtccgg ccgttcatcg tcaggatgtg ccaggtgaag gccaccagat
      361 cccgccaatg cccgtccggc aggaacagca ccgcggcggc gcccgcgtcg ccacgggaca
      421 acgcggcggc gaacgcatcc agccatgcgg tcgaggcggc gcgcgggtca gtatcgatgg
      481 cgttcgtcac ggtcagggcc cctggctact tcagggtatc gtttacaact gcgcggtgta
      541 ggaggaaaga gcgggctggc cgccgcccag aaattcccgc acgaacctgt tgaccggttt
      601 ggcatataga agctccggcg tgtccacctg caccagtcgg ccggcatgca tgacagccac
      661 ccgatcagcc atctccatcg cctcggcctg atcgtgggtt acgaacacgc tcgtcacgcc
      721 catccggtca tgcaattgcc gcagccagac ccgtagcgtt tttcgcacct gcgcatccag
      781 ggcgccgaac ggctcgtcca gcagcagaag cgccggctcg atcgccagcg cgcgagccag
      841 tgcgacgcgt tgtcgctggc cgccggagat ctgctccgga tagcggccgc cgaggtcacc
      901 gatctccatc aggtccagca acgcctgcac ccgcgcgcgg atctcccggt ccgatggccg
      961 ccgccgccgc ggcatgatct ccaggccaaa tgccacattc cgggcaaccg tcatgtggcg
     1021 gaacagggcg tagtgttgaa acacgaagcc gacgccgcgt tcccttgccg ggacgccggc
     1081 cattgatcgc ccgttgaaca ggatgtcgcc tgaatcgacg aattccagcc cggcgagagt
     1141 gcgcagcaac gtcgtcttgc ccgatcccga gggcccaagc aaagccagga attccccgtc
     1201 ggcgatgctc agggcgatgt cgttcaatgc agccacgccg ccgaacgact tgcgcaagcc
     1261 cagcagctcg atggtcacag caatgcccct gtccgctcgc cgatgtgcca ctctagtact
     1321 gccttgaccg ccagcgtcac cagcgccaac agcgacaaca atccagcgat ggtgaaggcg
     1381 ccgacccagt cgtaatcgtt gaacaaggct tccacctgca atggcatggt catggtctgg
     1441 cctcgcacat gtcctgacac cactgccacc gcaccgaact cacccatggc tcgggcgttg
     1501 cccagcagca cgccatacag caggccccag cgcacattgg gcaacgtcac gcgccagaac
     1561 atctgccaga acccggcacc gagcgtgatc gcggcttctt cctcggccga accctgctgc
     1621 tgcatcagcg ggatcagggt gcgtgcaacg aacgggaacg tgaccagtac cgtcgccaaa
     1681 atcaagcctg ggacagcaaa aatcacatgc agcccggctc ggttcagcag cgggccaaac
     1741 cacccattgg caccgaacag cagcacccac accacacccg agatcaccgg cgacaccgaa
     1801 aacggcagct cgatcagcgt gatcagcaga ctacggccgg ggaactggaa gcgtccgata
     1861 caccaggcag ctgccaatcc gcacaccgtg ttcaccggca tgctgatcgc cgcaaccagc
     1921 aacgtcagtt cgatcgccgc caccgtgtcc ggttcggcca acgcctgtcc gacggccgtc
     1981 cacccccgcg acagcgcttc ggtgaacacg acggccagcg gcaacagcag gaacatcgcc
     2041 agcagcagca ccgtcgccac tgtcagtccc agccgaaccg tccgggattc ccgtaccgca
     2101 tttggaaaaa ccgtggcgct catcgggttc gcaggcccat gcgccgctgc aggatattca
     2161 acagcatcag aatggtgaag gacaccagca gcatcaccag tcccagcgcg gcggcgccgg
     2221 cataatcgag ctgttccagc cggatcacga tcagcagcgg tgcgatttcg ctgatcatcg
     2281 gcatgttgcc ggcgataaag atgacggatc cgtactcgcc gacgcccctg gccagcgcgg
     2341 cggcgaagcc agccagcagg gcaggggcca gcgccggcag caccacccgg cgcagcgtct
     2401 gcgaccgcgt ggcacccaaa gtggcggcgg cttcctcggc attcagcggc aggtcgatca
     2461 gcaccggttg cagggtgcgc accacgaacg gcaggccgac gaagatcagc gccaccagga
     2521 ttcccagcgg cgtgtatgcc accttgatcc ccagcggcag aagccacgca ccgatccacc
     2581 ccttgggcga aaacaatgcg gtcaaggaga tcccggcgac ggcggtgggc agcgccagcg
     2641 gcatgtcgat cagcgagtca gccagccgcc gcccgggaaa cggataccgc accatgaccc
     2701 aggcgacgag cagcccgaat ggaacattca ccgctgccgc caacgccgcg gcgccaaaac
     2761 tcagccgcag cgctgccagg accctcggtt cgctcaccgc atgccacacg cccgccgccc
     2821 caagctccca cgggcgcagc gcgagcgaca ccagtggcag caagacgatc atcgataccc
     2881 aggccagggt gatcgtcagc gcgggggcga acccgggtaa ggcagagcgt cgggcacccg
     2941 tggttttccc gctcggcatt ttcccatccg gcatttcccc acccggcatt tccccacccg
     3001 gcattttccc gacgatcatt ttcccggcgt gtagatacgg tcgaacatcc cgccatcggc
//
LOCUS       DASWQN010000902         8683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_878383, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000902 DASWQN010000000
VERSION     DASWQN010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 8683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8683
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878383"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /locus_tag="VGC82_15330"
     CDS             complement(<1..1845)
                     /locus_tag="VGC82_15330"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY0424690.1"
                     /translation="MSPLIRGVTRVLAAIGVIVLLLAAVAAGAVWLTMPDTRQTASIP
                     GLSGPVAITYDADWVPRVSAGSEADAATAVGFIHARDRMFQMELMRRAASGRLSEIAG
                     PAALPIDRMMRTLGLRQHAVADLATLPVETRSVLEAYARGVNAWIDQKGRFAAPEFLV
                     LGPPEPWQPTDSLLWAKTMGLWLSMNWRQELSRQASAGRASPEVLDQLWPHQAGLPAP
                     DAMRQLGVPTRFASAASRLTAVLPQFPGAYTMPQSASNEWAVDGRHTATGAPLLAGDP
                     HLGFSFPGIWYLARIDMPGRVLAGATAPGVPFLVLGHNGKIAWTFTTTGADVQDVFIE
                     TPSGSGQYQTPDGPRPFVVREERIKIWGQADVVLTVRETRHGPVISDLDRPDGPARAD
                     APIQAVAMGNLQFGDTAAAGLLALNRAATVQEAGLAAGMISSPVQNLLVADAKTIGLF
                     VTGRVPIRKAGDGSAPIPGDGSHDWTGWASGNELPSSVSPASGRLVNANEPVWPRDFP
                     VFMGRDTFGDWRARRIREMLDRSDRHTPASFAAMQADAVNTFARQVLPALLTVPGVTG
                     TAAKALDLLRGWDGSAVMDQPAPLIFNAWMEAFRTAVLRHSGVTTGLGA"
     gene            2028..2516
                     /locus_tag="VGC82_15335"
     CDS             2028..2516
                     /locus_tag="VGC82_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0424691.1"
                     /translation="MKPSSSDTPILLDAIDRRILAALQDDGRITNIELSKRAGISAPP
                     CLRRVRALEEAGVIRGYHADTDAYLLGWEITFFAVVGLDSQKEAVLSAFEQLVGTWAE
                     VRECHMIRGGGDFLLKLVARDTAHENQLTQKLTGQPTVSRVQTLQTIRTSRNLAGVPF
                     QA"
     gene            complement(2536..3747)
                     /locus_tag="VGC82_15340"
     CDS             complement(2536..3747)
                     /locus_tag="VGC82_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424692.1"
                     /translation="MMRRIFVLAVLALLDLAVFVMPAAFAADGSPTAPNPDTTSLNPK
                     TSSTPNVNCPNRPPAAEGAPSDMIVELDPNTLWRPRGSEVRFSIRGPGSAVAVKKVKV
                     CFGWSSPDAQFEDINALVGSPQVRPVINDVGATGYGAVVPNLKRLPDSVWWPRRLLDS
                     GHFVFSGVATVPVADMVVEVTPAEGPVIVTSIQVGVTAAIVAWVVVAVVAIMLWAVLH
                     GIAKHRRIRGRYLLLRVISTQDGYASLSQLQIVLWTLVVGMSAIYVMTLSGNLISISE
                     GTLILLGIASGAALLARASPVVQPAGAAPATQAASVTPVLDPPANPAMPEWADLIIPN
                     RQTQEIDVTRLQMLAFTLITAAFVVIKVVVDYEIPAIPPNFLILMGISNGVYVAGRSL
                     PSRPRDGTTTG"
     gene            complement(3925..5229)
                     /locus_tag="VGC82_15345"
     CDS             complement(3925..5229)
                     /locus_tag="VGC82_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0424693.1"
                     /translation="MNLYRLLQRRAASGNPVRVGLIGAGKFGSMFLSQVPTTPGLEVA
                     AIADLSPDRARQACRNVGWTEERIAATRFVDDAAAMIAFPQVEVVVEATGHAPAGISH
                     ARRAIREGKHIVMVNVEADVLAGPLLAREAEAAGVVYSLAYGDQPALICELVDWARSC
                     GFPVISAGKGTKYMPEYHTSTPETVWGYYGLTAEQAKIGGMNPQMFNSFLDGTKSAIE
                     MAAVSNASGLTPAPDGLVFPPVGTHEMAEMLRPGNAGTLHHSGQVEVISSVHRDGSPV
                     ENDLRWGVFVVFEAPNDYARRCFQEYGVVTDRSGRVSALYRPFHLIGLELNVSILSAA
                     LRKEPTGTPTGFRGDVVSTAKRDLRANEVLDGEGGACVWGKLMTAADSLRRGGLPIGL
                     ANRVPLVRDVAAGQPVTWDDVRIDTADEAYKYRREMEAAFPT"
     gene            complement(5320..5760)
                     /locus_tag="VGC82_15350"
     CDS             complement(5320..5760)
                     /locus_tag="VGC82_15350"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY0424694.1"
                     /translation="MLASWLFARPSDAPAHAPDNGHISAGAGDLAVLDGATLRVGEHV
                     VVLEGILAPARGSVCHGDRRTELDCGAAAANALASLVRARGVDCAIRSHDARGRPVGD
                     CVVVGKKLSATLVWDGWARAQADDLREAEAAARASGRGIWHPAS"
     gene            complement(6171..6707)
                     /locus_tag="VGC82_15355"
     CDS             complement(6171..6707)
                     /locus_tag="VGC82_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0424695.1"
                     /translation="MRQLMLLRHAKSTWDDASVPDRDRPLNARGHRSAAIMRRAMRNL
                     GLAPDVVLVSTARRAMETLEALEPWDDTPLVEPMDSLYLANPIQLIAALHGVAETVRS
                     VLLIGHNPGLHDLALTLAGPGAIAGGGETARALAAGFPTAALVEFVVAGSWWDLREGG
                     GRLVRFLTPRMLEVAGQS"
     gene            complement(6928..7608)
                     /locus_tag="VGC82_15360"
     CDS             complement(6928..7608)
                     /locus_tag="VGC82_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0424696.1"
                     /translation="MTAPVIPSGAKIRVVGDVHGDATGFAYAIATDRFIVQLGDLTDH
                     GPNSAGTLRMMFDLIERGRGLFILGNHDLKLGRVLSGDQVRIDPVVQATVEQLDDDLK
                     TRTLRLVAEAPAWLRHGPALFVHGGFHTAMLEQASPDHGLARPQGPLARALYGEPTGR
                     IQPDGFPERSLRWVDRIPEGLTVYCGHDRRSSDGRPYVRYGQLGGKAVFLDTGAGKGG
                     HLSWIDLQ"
     gene            7832..>8683
                     /locus_tag="VGC82_15365"
     CDS             7832..>8683
                     /locus_tag="VGC82_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEY0424697.1"
                     /translation="MATHRIASTPETVRWGVFDANFPPLITIDSGDTIVLECLSGGPE
                     VMPPPGSDLTVPPALAAIHAANIPRAPGHLLTGPVGVAGAEPGDMLEVRIDRIEFGSD
                     WGYCGFRPLAGTIPEDFPEKYLSHIPVDRERRTCRLPWGTELNLAPFFGVMGVAPPPA
                     YGTISTVQPREHGGNLDNKELGEGSTLFLPVWAPGALFSAGDGHGVQGDGEVCINALE
                     ICLTGTFTLTLHKGNGARAPILSYPRAETASHFISMGMHEDLDVAMKTALRQMIAFIT
                     SRTNLSRQ"
BASE COUNT         1577 a         2968 c         2691 g         1447 t
ORIGIN      
        1 cgcgcccagg cctgtcgtca cgccggaatg gcgaagcacc gcggttcgga atgcctccat
       61 ccaggcgttg aagatcaacg gtgcgggttg gtccatcacc gccgatccat cccagcctcg
      121 caacaggtcc agcgccttcg cggctgtgcc ggtcaccccc ggcaccgtca gcaacgccgg
      181 cagaacctga cgggcgaagg tgttgaccgc gtcggcttgc atggcggcga agctcgcggg
      241 cgtgtgccgg tccgaccggt ccagcatttc gcgaatacgc ctggcccgcc aatcgccgaa
      301 cgtgtcgcgt cccatgaaaa cgggaaagtc gcgcggccat accggctcgt ttgcgttgac
      361 caggcggcca ctggccggtg agacactgga cggcagctcg ttgcccgatg cccaaccagt
      421 ccagtcgtgc gagccatccc ccggaatggg ggcggatccg tccccggcct tgcggatcgg
      481 cacgcgtccg gtgacgaaca gcccgatggt cttcgcatcg gcaaccagca agttctgaac
      541 cggggaactg atcattccgg cggccaaacc cgcctcctgc accgttgccg cccggttcag
      601 cgccagcaat ccggccgcag ccgtgtcgcc gaactgcagg ttgcccatcg ccacggcctg
      661 gatcggcgca tcggccctcg ccggcccatc cggacggtcc aggtcgctga tcaccgggcc
      721 gtgccgcgtt tcgcgcaccg tcagcaccac gtcggcctga ccccaaatct tgatccgctc
      781 ttcccgcacg acaaacggcc taggtccgtc aggggtctgg tattggcccg aaccggaagg
      841 cgtctcgatg aaaacgtcct gcacgtcggc accggtggtg gtgaaggtcc aggcgatctt
      901 gccgttgtgc cccagcacca ggaacggcac gccaggcgcc gttgcgcccg ccaacacccg
      961 ccccggcatg tcgatccggg ccaggtacca gattccgggg aagctgaacc ccaaatgcgg
     1021 atcgccggcg agcagcggcg cgcctgtcgc ggtgtggcgg ccatcgacag cccactcgtt
     1081 ggatgcgctt tgcggcatcg tgtaggcacc cggaaattgc ggcagcaccg ccgtcaggcg
     1141 cgacgcggcg gaggcaaatc gggtcggtac ccccaactgt cgcatcgcgt ctggtgccgg
     1201 caatccggcc tgatgcggcc aaagctgatc gagcacctcc ggcgaagcgc ggccggccga
     1261 agcctgccgg gacagttcct gccgccagtt catcgaaagc cacagcccca tcgttttcgc
     1321 ccacaacagg ctgtcggtcg gctgccatgg ttcgggagga cccagcacca ggaactccgg
     1381 cgcggcgaag cggcccttct ggtcgatcca ggcatttacg ccgcgcgcgt aagcctccag
     1441 caccgaccgt gtctcgactg gcaaggtggc gaggtccgcc accgcatgtt gccgcaggcc
     1501 cagcgtgcgc atcatccggt cgatcggcag cgccgccggc ccggcgatct ccgacagccg
     1561 gcccgacgcc gcccggcgca tcaactccat ctggaacatc cggtcgcggg catggatgaa
     1621 ccccaccgcc gtggcggcgt ccgcctcgga tccggcgctg acccgcggca cccaatcggc
     1681 gtcgtaggtg atggcgacgg ggccggaaag accagggatc gaggcggtct ggcgcgtgtc
     1741 cggcatggta agccacaccg cgccggcggc cacggcggcc agcagcagca cgatgacgcc
     1801 gatcgccgcc agcacgcggg tcacgcctcg gatcagggga gacatgtcgg caacctgacc
     1861 ggacggaccg gcgctgggaa gaggctaaat tcgcaaatcg ccgccgccgc cggaagcgcc
     1921 gccgccgctt tacgttccat ggcggcccaa tgtttcggct tgcgggtcgc gcagtacgca
     1981 ataagatcgt accctcccga ggccaggaaa cgtcgttcaa agcaatcatg aagccgtcga
     2041 gctcagacac gccgatcctg ttggacgcga tcgaccgacg catcctcgcc gccctgcagg
     2101 atgacgggcg catcaccaat atcgagctgt ccaagcgggc cggtatttct gccccgcctt
     2161 gcctgcgccg tgtccgagca ctggaggaag ccggcgtcat ccgcggctac cacgccgata
     2221 ccgacgcgta tctgctgggg tgggagatca cgttctttgc cgtggtcgga ttggacagcc
     2281 agaaagaagc ggtgctgtcc gcattcgagc agctcgtcgg aacctgggcc gaggtgcgcg
     2341 aatgccacat gatccgcggc ggtggcgact tcctgctgaa gttggtggcg cgcgacaccg
     2401 cccacgagaa ccagttgacg cagaagctga ccggccaacc cacggtcagc cgggtgcaga
     2461 cgctgcaaac gatccgcacc agccgcaacc tggcgggcgt cccgttccag gcgtgaccgg
     2521 ccaccaacct caaggctatc ctgtcgtggt gccatcccgc ggtctgctcg gcaggcttcg
     2581 gccagcaaca tagaccccgt tgctgatgcc catcaggatc aggaagtttg gtgggatcgc
     2641 cgggatctcg tagtccacca cgaccttgat gacgacgaac gctgccgtta tcagcgtgaa
     2701 ggccagcatc tgcaggcggg tgacgtcgat ctcctgtgtc tgcctgttgg gtatgatcag
     2761 gtcggcccat tccggcatgg ccggattggc cggcggatcc agtaccggtg tcaccgaagc
     2821 ggcttgcgtt gcaggcgcag ctccggccgg ctgaacgaca ggcgacgccc gtgccagcaa
     2881 cgcggcaccg ctggcgatcc ccagcaggat caaggtgcct tcgctgatgc tgatcaggtt
     2941 gccggacaac gtcatgacat agatcgccga catcccgacc accaacgtcc acagcacgat
     3001 ctgcagttgc gacaggctgg catagccgtc ctgcgtcgag attacccgca gcagcaggta
     3061 tcttccccgg atccgtcggt gtttcgcgat cccgtgcaac accgcccaga gcatgatcgc
     3121 gacgaccgcc accaccaccc aggcaacgat agcggcggtc acaccgacct ggatcgaggt
     3181 cacgatcacc ggcccctccg ccggcgtcac ctccacgacc atgtcggcga ccggcacggt
     3241 ggccactccg ctgaagacga aatggccgga gtcgagcagc cgccgcggcc accatacgct
     3301 gtccggcagg cgcttcaggt tcggcaccac agcgccatat ccagtggcac caacatcgtt
     3361 gatgaccggg cggacctgcg gactgccgac cagggcgttg atgtcctcga attgggcatc
     3421 gggactgctc cagccgaagc acaccttgac ttttttcacc gcgacggcag agcccgggcc
     3481 tcggatgctg aaccgcacct cgctgccgcg cgggcgccaa agcgtattgg ggtcaagttc
     3541 cacgatcatg tcagacgggg cgccttcggc tgccggcggc cgattcgggc agttgacgtt
     3601 cggcgtcgag gacgttttcg ggttaagcga cgtcgtgtcg ggattcgggg cggttggcga
     3661 accgtcggcc gcaaatgcgg ccggcatgac gaagaccgcc agatcaagca gcgccaggac
     3721 agccagcaca aatatgcgac gcatcatccc accatgtttt aatggcaggc tacatcgttt
     3781 acctccacaa tcaacttgtt ccggtctggc ctggaccatg atcgtttgag gttgaaccct
     3841 ccaggacgtc cgatcatggt ctgaccctgt gcttgatcgc gtgattcacg gcttccgact
     3901 gggccgacct caggcggtca cgctctaggt cgggaaagcc gcttccatct cacggcgata
     3961 cttgtaggct tcatcggccg tatcgatccg cacgtcgtcc catgtcaccg gctggccggc
     4021 ggccacgtcc cgcaccaggg gcacccggtt cgccaacccg atcggcaacc cgccgcgtct
     4081 aagactgtcg gccgcggtca tcaacttgcc ccacacgcag gcgccaccct cgccgtcgag
     4141 cacctcattc gcccgcaggt cgcgcttggc ggtcgagacc acgtcgccgc ggaaaccggt
     4201 gggcgtcccc gtgggctcct ttcgcagtgc cgccgacagg atcgatacat tcaattccaa
     4261 cccgatcagg tgaaacgggc gatacagcgc cgacacccgg ccggagcgat cggtgacgac
     4321 gccgtactcc tggaagcacc gccgggcgta atcgttcggc gcctcgaaca ccacgaacac
     4381 gccccagcgg agatcgttct ccaccgggct gccgtcacga tgcaccgatg aaatgacctc
     4441 cacttgcccg ctgtggtgca gcgttccagc gttgcccggt cgcagcatct cggccatttc
     4501 atgggtgccg accggcggga acaccagtcc atccggcgcc ggcgtcaggc cggatgcgtt
     4561 ggaaaccgcc gccatctcga tcgccgactt cgtgccgtcg agaaacgagt tgaacatctg
     4621 cgggttcatc ccgccgatct tcgcctgttc cgccgtcagc ccgtaatacc cccaaaccgt
     4681 ttcgggcgtg gaggtatggt attccggcat gtatttggtg cccttgccgg cggaaatcac
     4741 cgggaagccg caagatcgcg cccagtccac cagttcgcag atcaatgccg gctgatcgcc
     4801 ataggccaga ctgtagacga cgccggcggc ttcggcttcg cgcgccagca gcgggccggc
     4861 caggacgtcg gcctccacgt tcaccatgac gatgtgtttg ccttcgcgga tggctcgtcg
     4921 agcatgagaa atgccggccg gggcgtgacc ggtggcttcc accacaacct cgacctgcgg
     4981 gaaagcgatc atcgccgcgg cgtcatcgac gaagcgagtg gcggcaatgc gttcctcggt
     5041 ccagccgaca ttgcggcacg cctggcgggc gcggtcgggt gacaggtcgg cgatggcggc
     5101 gacttccagt cctggcgtgg tcggcacctg cgacaggaac atcgagccga acttgccggc
     5161 gccgatcaac ccgacacgta cggggtttcc actggcggcg cgccgttgca gcaggcgata
     5221 aagattcatg tctgtcctcc cggtccgatc gtcgcccgct tctacaggcg gtggcggaaa
     5281 atgcgaacag ggtgcagccg tccgtcagtc tgaacaggcc tacgaagcag gatgccaaat
     5341 cccccggcca gaagctcggg ccgcggcttc cgcttcccgc aagtcgtccg cctgcgcccg
     5401 agcccagcca tcccaaacga gcgtggcgct gagcttcttg ccgaccacga cgcaatcgcc
     5461 cactggccgg ccacgcgcat catgactcct gatcgcgcaa tccacgcccc gggcccgcac
     5521 gagcgacgcg agcgcattcg ccgccgccgc gccgcaatcc agttccgtcc ggcggtcgcc
     5581 atggcagact gaccctcgcg ccggtgccaa tatgccttcc aacaccacga catgctcgcc
     5641 gacccgcaaa gtggcgccat ccagaacggc cagatctccg gcaccggcgg agatatggcc
     5701 gttgtccgga gcgtgggcag gagcatccga cggacgcgcg aacagccaac tggcgagcat
     5761 cacggttgcc actccagcaa ccacggcgac gagcacgcct ggcgacggta acgccctacg
     5821 ccggcgcgga gccggaccag taccggaccg gaaaatccgc cgcctatggt tcaacgacac
     5881 ggtcggtacc ccgatggttc tggcgctggc atatcccggc caacgatcca ggcgatcgat
     5941 tcgccgaatc ccgccagcaa aggtcaagtc caaaattcgg cttgcgctca gaattggtcg
     6001 aacgcccgga tgatccgccg cgggatcttt tccgcccatg ccgccatcaa tccagccacc
     6061 agcccgcgcc gcaggcctgg ccagctcgtg gtcagggctt tggatcgtcc gcgtaggaca
     6121 accaaggcca ggccggcggt aagctcgagt tcaatggaat ccgaagtcca tcaggattgg
     6181 ccggcgacct ccagcatccg gggcgttagg aatcgcacca gtcggccacc gccctcacgc
     6241 aggtcccacc acgacccggc tacgacaaat tcgaccaagg cagcagtcgg gaagccggca
     6301 gccagcgcgc gtgcggtctc gccgccgccg gcaatcgcgc cgggtcccgc cagagtcagc
     6361 gccagatcgt gcaggccggg attgtggccg atcagcagga cgctgcgaac tgtctccgcc
     6421 accccgtgca gtgccgcgat caactggatc gggttggcca gatagagact gtccatcggc
     6481 tcgaccagcg gggtgtcgtc ccagggttcc agggcttcca gagtttccat cgcccttcgt
     6541 gcagtggaaa ccaggacgac atcgggtgcc agccccaagt tacgcatcgc ccgccgcatg
     6601 atcgccgcgg atcgatgccc gcgcgcgttc aggggtcggt cccggtccgg caccgaggcg
     6661 tcgtcccagg tggacttcgc gtgtcgcaaa agcatgagct ggcgcatgtt cgcggcacct
     6721 tgccacgcct ggcgacgttt gtcctccccc ggcgcactaa aaccagggtt gcgtaccaat
     6781 gtcatgaacc gggtttgtca tgaactgggt tcgcgaagcg ggccgcggcg catccgaagc
     6841 attccgtgtc ccgatcgagg acgagcaatc tggcggtcaa gccgcatgag gtctcgctga
     6901 tggccaggga tcaccaggcg aaggcgccta ctgcaggtct atccaggata gatgtccgcc
     6961 tttccctgcc ccggtgtcca agaacaccgc cttgccgccc agttggccat agcgcacgta
     7021 aggccgccca tcgctgctgc ggcggtcgtg gccgcaatag accgtaaggc cctccggaat
     7081 ccggtcaacc cagcgcaggc ttcgttcagg aaatccatcc ggctggatcc ggccagttgg
     7141 ttcgccatac agagcccgcg ccagcgggcc ttgcggacgg gccaggccgt gatccggcga
     7201 ggcctgttcc agcatcgccg tgtgaaaccc gccgtggaca aagagcgccg gaccatgccg
     7261 caaccaagcg ggtgcctcgg ccaccagccg gagcgtccgg gtcttcaggt cgtcgtccaa
     7321 ctgttcgacc gttgcctgca cgaccgggtc gatccgcacc tggtcgccgg acagcacgcg
     7381 gcccagctta aggtcgtggt tgcccaggat aaacaaacca cgtccgcgtt cgatcaggtc
     7441 gaacatcatc ctcagcgtgc ccgcgctatt ggggccatgg tccgtgaggt caccgagctg
     7501 cacgatgaac ctgtcggtgg cgatggcata agcaaagccc gtggcatcgc catgaacatc
     7561 gccgacgaca cgtattttcg caccggaagg gattacggga gcagtcatcg aattgttatg
     7621 tagataggtt gatgcttcga accaaccagg caattggtcg cgacctggcg attttcagcc
     7681 tcgggatata tctcggcacg tgacgatcag tggcgtgcgc caatcacgcg cagcgttcac
     7741 aacccgcccg ggaccgtgat ttcaccggtt gtgccaccgg ggtcgatgtg gtggcatcgg
     7801 gaatcatcgc tgctgccgag aggaccgccg aatggccacg catcgcattg catcgacacc
     7861 cgagaccgtg cgctggggcg ttttcgacgc taacttccca ccactgatca cgatcgattc
     7921 aggcgatacc atcgtgctgg aatgcctatc cggcggcccc gaagtgatgc ctcccccggg
     7981 ctcggactta accgtgccac cggctttggc cgcgattcat gctgccaaca ttccacgtgc
     8041 ccccgggcac cttttgaccg ggccggtcgg ggttgccggc gccgaacccg gcgacatgct
     8101 ggaagtgcgt atcgacagga tcgagttcgg cagcgactgg ggttactgcg ggtttcgtcc
     8161 gctggcgggc acaatcccgg aagatttccc ggaaaaatat ttgtctcata tcccggtgga
     8221 tcgggagcgc aggacgtgcc gcctgccatg gggaaccgag ctgaatctcg cgccgttttt
     8281 cggcgtgatg ggcgtggcac cgccgcctgc ctatggtacg atttcgaccg ttcaaccgcg
     8341 tgagcacggc ggcaacctcg ataacaagga actcggcgaa ggttccacct tgttcctgcc
     8401 agtctgggcg cctggcgcat tattttctgc cggcgacggc cacggcgtgc agggcgacgg
     8461 cgaggtctgt atcaacgcgc tggaaatatg cctgaccggc acgtttactc tgaccctgca
     8521 taagggaaac ggcgcgcgcg cgcccatact ttcctatccg cgagcggaaa ccgccagcca
     8581 ttttatttca atgggcatgc acgaggatct ggatgtcgcg atgaagaccg cattgcgcca
     8641 gatgatcgcc ttcatcacca gccgcaccaa cctgtcccgc cag
//
LOCUS       DASWQN010000903         5048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2066087, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000903 DASWQN010000000
VERSION     DASWQN010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066087"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..2915)
                     /gene="ligD"
                     /locus_tag="VGC82_15370"
     CDS             complement(207..2915)
                     /gene="ligD"
                     /locus_tag="VGC82_15370"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEY0424698.1"
                     /translation="MARSAASQRDTSIQTYRQKRDFTATTEPQPSERAPQKNQAPIFV
                     VQKHQAHRAGLHWDFRLEHNGVLWSWAVRKGPSLDPADKRIAAHVEDHPLDYASFAGT
                     IPDGHYGAGKVEIWDRGTWEALIDPDDGLRDGEIKFVLSGKRLRGKFTLVRLKPKPNQ
                     RNQDNWLLIKGLDGLQQQGADAEALEAAIEPPGKPNGTSREPPIPGAVHGKLPDQQAP
                     QLAAVADGPPEEDGWLSEIKFDGYRLIASLDRSADAEQGQVRLLTRNGNDWTDRLPAV
                     ARLIDQLHADRAMVDGELVALDKDGTSSFPALQAALSGGHDQTLMFYLFDLLHLDGWD
                     LRRCALRERKQRLHGLATWQGMLRYSDHHEGDVVRMRQAACRMKLEGIICKRGDAPYR
                     AGRGQDWLKVKCQGREEFVVIGWTEPAGSRAGLGSLHMGYYDPSGALHYAGGVGSGFS
                     DDMLAILRDRLDGLGASPPRGLIVAGEELERTIHWVRPELVAETSFTAWSGAGRMRHG
                     VFLGLREDKAARDVVRDPADPDAERRAVTPRDHVGSAKRKGPIIAVPPKRETTKLPPA
                     PSPRDTPLRTGSSIVTAKAPGARTETIEGVTLTHPDRALWPGITKRDLAGYWQAVADH
                     ALPGLAHRPLAIVRCPEGVDGEHFFQKHGHGTLPPGFRDGEADKAPFLVIEGLQGLVA
                     AAQMSAVELHVWGATEADPLHADQLVFDLDPGDGVKVSEIVAAAFDMREKLEAIGLAS
                     FCRTSGGKGLHVVVPLVADQTWDTVRAFCRSFAESASEASPEKYLAHVKIADRRGRIL
                     IDWLRNGLGSTAVASFCPRARPGAGVATPLAWTEVTPKLDLQAFTLKTVPGRLGKLKR
                     DPWEGFEEARRSLPAAPKQTAKAKVQPAGKSVIVTAKAPKPRVKRS"
     gene            3084..4172
                     /gene="dusB"
                     /locus_tag="VGC82_15375"
     CDS             3084..4172
                     /gene="dusB"
                     /locus_tag="VGC82_15375"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY0424699.1"
                     /translation="MGNCRSQITPSNLGSIDLGCGIRIETPVILAPMSGVTDLPFRRT
                     AKQLGAGMVVSEMIASWAMIRENQKTLGMAEIDGCGGVSAVQLAGCDPAAMAEAARLA
                     VDRGAHLIDINFGCPVKKVAVGQAAGSALMRDEAAAAAILEATVKAVSVPVTLKMRMG
                     WDHANLNAPRLARIAQAAGIRMVTIHGRTRQQFYNGAADWGFVRHVKQEVDIPVIVNG
                     DILTEDDAVAALAQSGADGVMIGRGCYGRPWFLAQAAHYLRTGTRLPEPSLARQKAIL
                     LEHYQAMLSQFGTGPGVRLARKHVSWYSRGLPGSAEFRATMNRLPDAISVLALIDRFY
                     DPLIARDVVRTAPAGTFGDDDTSAAEAA"
     gene            4169..>5048
                     /locus_tag="VGC82_15380"
     CDS             4169..>5048
                     /locus_tag="VGC82_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY0424700.1"
                     /translation="MSLGVTRATRFLGGRGRTIALPTKAVDAAAILGALPVPVILLDV
                     ENRFRHVNHAAEQFLGISAAGLALLGLSDLLPSDNPLFLLIEQVRRTEATISDHDLTL
                     ESPRLHKRGITVQGSPLPEEPGAVLLVIQDASAARSLDRQLAFRGAARSVTGMAAILA
                     HEVKNPLSGIRGAAQLLEASVAPADRELAVLIRDEADRIRDLVDRMEAFGEKPIARTA
                     VNIHRVLEHVRKLAQTSFAAHIRFHELYDPSLPPVWANRDQLVQVVLNLVKNASDAVT
                     QEGIHHPEITLTTGFQH"
BASE COUNT          865 a         1620 c         1662 g          901 t
ORIGIN      
        1 cctgaaggac cacacgaaca aggacctctt gcaggcatac gagaacatct gaataccaat
       61 gcgatgacgc ctgcgaaagg cgtggatgcc gaccttcgcc ggcataacga ggtggtgcgg
      121 caaaagcggt cgatctcaac gcgagctggt atgacgcagg taagcaccag catcccgacc
      181 cgtcgtcgcc tgcggaattc ctgtggctac gaccgcttca cccgcggctt cggcgccttc
      241 gccgtcacga tcacggattt tccggccggc tgcactttcg ccttcgccgt ctgcttcggt
      301 gccgccggca gacttcgccg ggcttcttcg aaaccttccc aggggtcccg tttcagtttc
      361 cccagccgcc ccggcacagt cttcagcgtg aacgcctgga ggtcgagctt cggcgtcacc
      421 tccgtccacg ccaaaggcgt tgccacgccc gcgccaggcc gagcgcgggg acagaacgaa
      481 gcgacggccg tggaacccag tccgttgcgc agccagtcga tcaggatacg gccgcgccgg
      541 tcggcgatct ttacatgcgc caaatatttt tccggactgg cttcgctggc cgactccgcg
      601 aaggaccggc aaaacgctcg caccgtgtcc catgtctggt cggcaaccag cgggacaacg
      661 acgtgcaggc ccttgccgcc gctggtgcgg caaaacgatg ccagcccgat cgcctccagc
      721 ttttcacgca tgtcgaaagc ggcggcaaca atttcagata ccttcacgcc gtcgcccggg
      781 tcgaggtcga agacaagctg gtcggcgtgc agcggatcgg cttcggtggc gccccagacg
      841 tgaagttcga cagcggacat ttgcgccgcc gcgaccaggc cctgcagccc ttcgatcacc
      901 aggaacgggg ccttgtcggc ctcgccgtcg cggaagccag ggggcaacgt gccatggccg
      961 tgcttctgga agaaatgttc accatcaaca ccttccgggc agcggacgat cgccaggggg
     1021 cggtgtgcca gaccgggcaa agcgtgatcg gcgaccgcct gccagtatcc cgccaggtcg
     1081 cgcttggtga tgccgggcca cagcgcgcgg tcgggatggg tcagggtgac gccttcgatc
     1141 gtctcggtcc gggcgcctgg cgctttggcg gtcacgatgg aactgccggt gcggagcggc
     1201 gtgtcgcgcg gtgatggcgc cggggggagc ttggtggttt cgcgtttcgg gggcacggcg
     1261 atgatcggtc cttttcgttt ggcggagccg acatggtccc ttggagtgac ggcacggcgt
     1321 tcggcgtcgg gatcggcggg atcgcgtacg acatccctgg ccgccttgtc ctcacgcaac
     1381 ccaagaaata cgccatggcg catccgcccg gcgccggacc aggcggtgaa ggacgtttcg
     1441 gccaccagtt ccggccgcac ccaatggatg gtgcgctcca gttcctcgcc ggcgacaata
     1501 agtccccgtg gcggagaagc gccgaggccg tcgaggcggt ctcgaaggat cgccagcatg
     1561 tcgtctgaaa atccgctgcc gacgccgcca gcgtagtgca gcgcgccgct cggatcatag
     1621 tatcccatgt ggagggaacc caggccggcg cggctgccgg cgggttccgt ccagccgatg
     1681 acaacaaact cttcgcggcc ctggcacttc accttgagcc aatcctggcc gcggccggcg
     1741 cgataaggcg catcacccct tttgcagatg atgccttcca gcttcatccg gcaggcggcc
     1801 tggcgcatgc ggacgacgtc gccctcgtga tgatcgctgt agcgcagcat gccttgccag
     1861 gtggccagac cgtgcaggcg ctgcttgcgc tcgcgcaggg cgcaccgtcg aagatcccag
     1921 ccatcgaggt gcagcaggtc gaacaggtag aacatcaggg tctgatcatg gccgccggac
     1981 agcgctgcct gcagcgccgg gaagctcgag gttccgtcct tgtcgagcgc gacgagttca
     2041 ccatcgacca tcgctcgatc cgcatgaagc tgatcgatta ggcgggccac ggccggaagg
     2101 cgatccgtcc agtcgttgcc gttgcgcgtc agaagccgca cctgtccttg ctccgcgtcg
     2161 gcgctccggt cgagggacgc aatcaggcgg tagccgtcaa atttgatctc gctcagccag
     2221 ccgtcctctt ccggggggcc atcggcgacc gcggcgagtt gcggggcctg ttgatccgga
     2281 agcttgccgt ggacagcgcc agggatgggt ggttcccttg atgtgccatt tggcttcccc
     2341 ggcggctcga tcgcggcttc caaggcctcg gcgtccgcgc cttgctgttg caggccatcg
     2401 agccctttga tcaacagcca gttgtcctgg ttccgctggt tgggcttggg cttcaggcgc
     2461 accaaagtga acttgccgcg caaccgcttg cccgacagga cgaacttgat ttcgccgtcg
     2521 cgaagaccgt cgtcggggtc gatcagcgct tcccaggtgc ctcgatccca gatctcgacc
     2581 ttgccggcgc catagtggcc gtcggggatg gtgcctgcaa agctggcgta atctagcgga
     2641 tggtcctcga catgcgccgc gatccgcttg tcggcgggat ccagcgacgg acctttgcgc
     2701 accgcccaac tccacaggac cccgttgtgt tccaggcgga aatcccagtg caggccggcg
     2761 cggtgagcct ggtgcttctg caccacgaag atcggtgctt ggttcttttg cggtgctcgt
     2821 tcggaaggtt gcggctcggt cgtcgcggtg aagtctcgct tttggcggta ggtttggatg
     2881 gaggtgtcac gttgcgacgc agcagaccga gccatggaag cgccttagat atgatgaacc
     2941 gaaacactgt agcactggag ccggtttcat cccacggccg aacagccaat tgcagggtga
     3001 tcatttcgcg gtcacgcggg cgctcgactt cggtcggatg agacattgcg ccttgatgaa
     3061 tgtggcgcta acctgcccaa atcatgggca actgtcgttc acaaatcaca cccagcaacc
     3121 tcgggtccat tgacctggga tgcggcatca gaatcgagac accggttatc ctcgcaccga
     3181 tgtccggagt caccgatctg ccatttcgcc gaaccgccaa acaattgggt gccggcatgg
     3241 tggtttccga gatgatcgcc tcctgggcca tgatccgcga gaaccagaaa accctgggta
     3301 tggcggaaat cgacggctgc ggcggcgtgt cggcggtgca acttgccggc tgcgacccag
     3361 ccgcgatggc cgaggcggct cggctggccg tcgatcgcgg cgcccacctg atcgacatca
     3421 acttcggctg tccggtcaag aaagtggccg tcgggcaggc ggccggatcg gcgctgatgc
     3481 gcgacgaagc ggcggcggcg gcgatcctgg aggcgaccgt caaagcggtg tcggtgccgg
     3541 tgacgctgaa gatgcgcatg gggtgggacc atgcgaacct gaacgcaccc cgcttggcaa
     3601 gaattgctca ggcagcaggc atccgcatgg tcacgatcca tggccggacg cgacagcaat
     3661 tctacaacgg cgccgccgac tggggctttg tgcggcatgt caagcaagag gtggacatcc
     3721 ccgtcatcgt caacggcgac atcctgaccg aggacgatgc cgtcgccgcg ctggcccaat
     3781 cgggtgccga tggggtgatg atcgggcgcg gatgctacgg gcggccgtgg ttcctggcgc
     3841 aggcagcgca ctacctgcga accggaacac gcctgccgga gccgtccctc gcgcggcaga
     3901 aggccatttt gctggagcac tatcaggcga tgctgagcca gttcggcacc ggccccggcg
     3961 taaggctcgc ccggaaacat gtctcttggt attcgcgggg cctgcccgga tcggcggagt
     4021 tccgcgcgac gatgaaccgc ttgcccgatg ccatctcggt gttggcgctg atcgaccggt
     4081 tctacgatcc gctgatagcc cgggacgtcg tgcgaacggc gccagcggga accttcgggg
     4141 acgacgacac ctcggcggcc gaagcggcat gagcctcggg gtcacccgcg ccacacgctt
     4201 cctcggcggg cgtggccgga cgattgcgct gccgaccaag gccgtcgacg cggccgcgat
     4261 tttgggcgca ttgccggtgc cggtcatcct gcttgacgtg gaaaatcggt ttcgccacgt
     4321 caaccacgcg gcggagcagt tcctcggcat ctcggccgcc ggtttggcat tgcttggact
     4381 cagcgatttg ctgccgtcgg ataatccgtt gtttctgctg atcgagcagg tccggcgcac
     4441 cgaggcgacg atctcggacc acgaccttac tctggaaagc ccgcggctgc acaagcgcgg
     4501 catcaccgtg cagggctcgc cgctgccgga ggagccgggg gcggtgctgc tggtcattca
     4561 ggatgcctcg gccgcgcgat cgctagaccg gcaactcgcc ttccggggtg ccgcccgcag
     4621 cgtgacgggg atggccgcga tcctggcgca cgaggtgaaa aacccgttat ccgggattcg
     4681 cggtgccgca cagttgctgg aagccagcgt cgcgccggcg gaccgtgaac tggccgtgct
     4741 gatccgcgac gaagccgacc gcatccgcga tctggtggac cggatggaag ccttcggaga
     4801 aaagccgatc gcccgcacgg cggtgaacat ccaccgggtg ctggagcatg tccgcaaact
     4861 ggctcagaca agtttcgccg cgcatatacg gtttcacgag ttgtacgatc cgtcgctgcc
     4921 tccggtctgg gccaaccgcg accaactggt tcaggtggtg ctgaacctag tgaagaacgc
     4981 ctccgacgcg gtgacgcagg aaggcatcca ccatccggaa atcactttga ccacgggctt
     5041 ccaacacg
//
LOCUS       DASWQN010000904         4955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2197887, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000904 DASWQN010000000
VERSION     DASWQN010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2197887"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VGC82_15385"
     CDS             complement(<1..560)
                     /locus_tag="VGC82_15385"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0424701.1"
                     /translation="MLLAGVGRAADPQIGTLLQSPVGKDWVTNGGTLTNQRYSTLKQI
                     DTSNVKQLKGAWMTRLKGSGYGGKYSFEATPLVKDGIMYIVTGNDDVFAMNAKTGEFL
                     WERWSGIDQKLTSVCCGWDNRGLAMGEGMLFVGQLDANVVALDVKTGKEVWRTPVEDW
                     HNGYGMTAAPLYFDGIVYTGITGGEFG"
     gene            complement(985..4290)
                     /locus_tag="VGC82_15390"
     CDS             complement(985..4290)
                     /locus_tag="VGC82_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY0424702.1"
                     /translation="MPLQVALTHKTSYRYDRLVALGPQTIRLRPAPQTRTAILSYELR
                     VEPQPHFINWMQDPQGNHLARVVFPEKVERFEVTVDLVADMVTVNPFDFFLEPEAETF
                     PFSYDPVIDQELAPYRSAEAPGPRLAALLAAIPRGEQPTVDMLVGLNGLVQSKVAYIV
                     RLEPGVLTPEETLQGGTGSCRDSAWLLVQVLRNLGMAARFVSGYLIQLAPDVKPLEGP
                     PGPSSDFTDLHAWAEVYLPGAGWVGLDATSGLLCGEGHIPLAASPDPISAAPITGVTE
                     KAEVTFDVAMSVERIRETPRTTKPYSDAQWQAILARGLDVDRALKQSDVRLTMGGEPT
                     FVAADDMDAPEWNTDALGPTKRAYAGRLIRRLMARWAKGAVLTHSMGKQYPGEQLPRW
                     ALHCHWRADGEDVWNDKSLLASDDDTGDATAMDAALFARSLAERLQVDPALVMPAYED
                     IHYYLWKEHRLPANVLAEGSRLADPLERARLAKVFAAGLASPVGSVLPLRRVIDGDAR
                     RWQSGKWFFKGGEMFLVPGDSPVGLRLPLESLPWVDPEKAEHDLAPDPFAPREQLPTR
                     QALRPPAADHHGRDNFRPLPQEPPIYGGEAGGDPSIVRTALAVEARGGLLHIFFPPVF
                     AIEDWLALTAAVEETAAETGRKVVLEGYLPPEDERLLHFSVTPDPGVIEVNTHPTSSW
                     SEQVKLTEELYEEARLVGLATEKFNLDGRHVGTGGGNHVVMGALSPEDSPFLRRPHLL
                     KSVVGFWHNHPSLSFLFSGQFIGPTSQHPRVDEARQDSLAELEIAFAQVRARQPVAPW
                     VTDRLFRNILADMTGNTHRTEFCIDKMYSPDSASGRRGLVECRAFEMPPHARMSAAQV
                     LLMRSAVAAFWERPYERSLVHWGTRVHDDFMLPHFVEQDFRGALEELASLGFGLDPDW
                     FAPHLAFRFPHIGQVAFRGIELELRHALEPWHVLGEEQSASGTVRYVDSSVERVQARV
                     SGWVDERYVLTANGAGVPLTPTDRDGEFVGGVRFKAWNPPSGLHPTIGVHAPLVLDVF
                     DRWSGRSLGGLTYHISHPGGRSYETFPVNANEAEARRRARFFPFGHTPGSMREPVVRR
                     SREHPRTLDLRRV"
     gene            complement(4392..>4955)
                     /locus_tag="VGC82_15395"
     CDS             complement(4392..>4955)
                     /locus_tag="VGC82_15395"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0424703.1"
                     /translation="ILGVVLLHEQLRPRQWFGLGLGMSGVGLVVGHAAVESATRFQGL
                     ILAFIGVLGLVSGTLYYGRFCRGVPLLPGAAAQFLAAAVASAAGAWLLETPRMEWNGI
                     VVATVAWNTVMVSLGGMGLYFAMLGRGTAARASANFYLVPGTAALLAWIVLGERLTLL
                     AVSGLLVASAGCWLVNSNPRAGKAAAA"
BASE COUNT          882 a         1695 c         1549 g          829 t
ORIGIN      
        1 ccgaattcgc cgccggtgat gccggtataa acgatcccgt cgaaatagag cggcgcagcg
       61 gtcatgccgt aaccattgtg ccaatcctcg accggggtcc gccacacctc cttgccggtc
      121 ttgacgtcga gcgctacgac attggcgtcg agttggccga cgaacagcat cccctcgccc
      181 atcgccagcc cacggttgtc ccaaccacag cagacgctgg tgagtttctg gtcaatgcct
      241 gaccagcgct cccacaagaa ctcacctgtc ttggcgttca tcgcgaacac atcatcgttg
      301 ccggtgacga tgtacatgat gccgtccttc acgaggggcg tcgcttcgaa cgaatacttg
      361 ccgccgtacc cggagccctt caggcgggtc atccaggcgc ccttgagttg cttgacgttg
      421 ctggtgtcga tctgcttgag cgtcgaatag cgctggttgg tcagagtgcc gccattcgtc
      481 acccagtcct tgccgaccgg gctttgcagc aatgtgccga tctggggatc cgccgcccta
      541 cccactcccg ccagcaacac gcccaaagct gccgtgatca ttactcgctg tccgcgccgc
      601 attgtttcct ccccttttca tcggccgtta ccggttctcg attttgactg tcatttctcc
      661 gattatcgcc gatttcgtct gtccacttcg aacgataggc accatttcgc cctcttgaca
      721 acaatcctat tgatcgcagc gctgccgcgg cgtcctgcgc tccgtgacgg caaacttcgc
      781 cgtaccatcc gtcttgcggc ccgtcttgcg gcccccgacc aaccgggcat ggcagacggc
      841 gcttgggtct gcaggtcccc ggatcggatg aagtctttct tcagcttgaa ataggcaacg
      901 cgcggtcgca gacggcatca ggacgatgaa caagcgtcgt ccctcagcga cttgtccgat
      961 atcccgaact tcagaacgtg gactctaaac ccgccgcaga tccagcgtcc gcggatgctc
     1021 ccgcgaccgc cgcaccactg gctcgcgcat cgatccgggc gtgtgcccaa atgggaaaaa
     1081 ccgagcgcgc cgcctggctt cggcttcgtt ggcgttgacc gggaacgtct cgtagctgcg
     1141 cccgccgggg tgggagatgt ggtaggtcag cccgcccagg ctccgcccgc tccagcggtc
     1201 gaacacgtcc agcaccagtg gcgcatgcac gccgatcgtc ggatgcagcc cgctcggtgg
     1261 gttccacgcc ttgaagcgca cgccgccgac aaattcgcca tcgcggtcag tcggggtcag
     1321 cggcaccccg gcgccgttgg ccgtcagcac gtaacgttcg tccacccagc cggagacgcg
     1381 ggcctgcacg cgctcgaccg aactgtccac gtagcggacg gtgccggagg cggattgttc
     1441 ctcgcccagg acgtgccagg gctccagcgc atggcgcagc tccagctcga tgccgcgaaa
     1501 cgccacctgc ccgatatgcg ggaagcgaaa cgccagatgc ggcgcgaacc agtccgggtc
     1561 caggccaaac cccaggctgg cgagttcctc caatgcgccg cgaaaatcct gttcgacgaa
     1621 gtgcggcagc atgaagtcgt catgcacgcg ggtgccccaa tggaccagcg agcgttcata
     1681 cggccgctcc cagaatgccg cgaccgccga ccgcatcagc agcacctgcg ccgccgacat
     1741 ccgcgcgtgc ggcggcatct cgaaggctcg gcattccacc agcccgcgcc ggcccgaggc
     1801 actgtccgga gagtacatct tgtcgatgca gaactccgtg cggtgggtgt tgccggtcat
     1861 gtccgccagg atgttgcgga acagccggtc ggtgacccat ggcgcgacgg gctgcctggc
     1921 ccgcacctgg gcgaacgcga tctccagttc cgcaaggctg tcctggcggg cttcgtccac
     1981 tcgcgggtgc tggctggttg ggccgatgaa ctggccgctg aacaggaagg acaggctggg
     2041 gtggttgtgc cagaagccga ccaccgattt cagcagatgg ggccgccgca gaaatggcga
     2101 atcctccggc gacaacgccc ccatcaccac atggttgcca ccgccggtgc cgacgtggcg
     2161 gccatcgagg ttgaatttct ccgtcgccag gccaaccagc ctggcttcct catacagttc
     2221 ctcggtcagc ttgacctgtt cggaccagga ggacgtgggg tgggtattga cctcgataac
     2281 gccgggatcc ggggtgaccg agaaatgcag caacctttcg tcctcgggcg gcagatagcc
     2341 ctccagcacc actttgcggc cggtctcggc ggcggtttcc tccacggcgg ccgtcagcgc
     2401 cagccagtcc tcgatcgcga ataccggcgg aaaaaagatg tgcagcaaac cgccgcgcgc
     2461 ctccaccgcc agcgccgtgc ggacgatgct ggggtcgcct cccgcttcac caccataaat
     2521 cggcggttcc tgcggcagcg ggcggaaatt gtcgcggccg tggtgatccg ccgcgggtgg
     2581 ccgcagggct tggcgagtcg gcagttgctc ccgtggcgcg aatggatccg gagccaaatc
     2641 gtgttccgct ttctccggat ccacccacgg aagtgattcc agaggcaggc gcaggccgac
     2701 cgggctgtcg ccggggacga ggaacatttc gccgcccttg aagaaccact tgccggactg
     2761 ccagcgccgg gcatcaccgt cgatgacccg ccgcagcggc aacacgctgc cgacggggct
     2821 cgccaggcca gccgcgaaca ccttcgccaa gcgcgcacgt tccaggggat cggccagccg
     2881 ggagccctcg gccagcacgt tggccggaag gcggtgttcc ttccacaaat agtaatggat
     2941 gtcctcatac gccggcatca ccaaggccgg atccacctgc agccgttcgg cgagacttcg
     3001 cgcgaacagt gcggcatcca tcgccgtcgc atctccggta tcgtcatccg aagccagcag
     3061 ggacttgtcg ttccagacgt cctccccgtc cgcccgccaa tgacaatgca gcgcccagcg
     3121 aggaagctgc tcgccaggat actgcttgcc catgctgtgg gtcagcaccg cgccttttgc
     3181 ccaacgcgcc atcagccggc ggatcagccg cccggcgtag gcgcgcttgg tgggacccag
     3241 cgcatcggtg ttccactccg gcgcatccat gtcgtcggcg gcaacgaagg tcggctcacc
     3301 acccatcgtc agccgcacgt cgctctgctt caacgcccga tccacatcaa gtccgcgcgc
     3361 gaggatcgcc tgccattggg catcgctgta cggcttggtg gtgcgcgggg tttcccggat
     3421 ccgctcgacc gacatggcga catcgaaggt gacttcggcc ttttccgtca cgccggtgat
     3481 cggcgcggcg gagatcggat cggggctcgc ggcgagcggg atgtggccct cgccgcacag
     3541 caggccagag gtcgcgtcca gcccgaccca gccggcgccg ggcagataga cctcggccca
     3601 ggcgtgcagg tcggtgaaat cggacgacgg tcccggcggg ccttccagcg gcttcacgtc
     3661 gggcgcaagc tggatcaggt aaccggagac gaaccgcgct gccatcccga ggttccgcag
     3721 cacctgcacc agcagccagg cagagtcccg gcacgatccg gtgccgcctt gtaacgtctc
     3781 ctccggggta agaacgccgg gttccaggcg cacgatatag gcgaccttgc tctgcaccag
     3841 gccgttcagg ccaaccagca tatcgactgt cggttgttcg ccgcgtggaa ttgccgccag
     3901 cagcgcggca aggcgcggac ccggggcttc agcggaccgg tatggtgcga gttcctggtc
     3961 gatcaccgga tcgtagctga acggaaaggt ctctgcctcg ggttcaagga agaagtcgaa
     4021 cgggttcacc gtcaccatat cggcgacgag atcgaccgtg acctcgaacc gctcgacctt
     4081 ttccggaaac accacccgcg ccaggtgatt gccctgcggg tcctgcatcc agttgatgaa
     4141 atgcggttgc ggctcgaccc tcagttcata ggacaggatc gccgtacggg tctgcggcgc
     4201 ggggcgaaga cggatcgttt gcgggccaag ggcgacgagg cggtcgtagc gataggaggt
     4261 cttgtgggtc agggcgactt gcagcggcat tgggtgctcg agacaaaggg ggcgcacaac
     4321 gttttgcact gcagcacgcg tgaaggcaac gaggaaggac ggaccgcgcc atcgccgcga
     4381 ccggtcgcgc ttcaggccgc cgccgctttg ccggcacgcg gattggaatt gaccagccaa
     4441 caacccgcgg aagcgaccaa caacccgctc accgccagca gcgtgagccg ctcgcccagc
     4501 acgatccacg ccagcagggc cgcggtcccc ggcaccagat aaaaattggc gctggcccgg
     4561 gccgcggttc cccggcccag catggcgaaa tacagcccca tgccgcccag ggataccatg
     4621 accgtgttcc atgccacggt ggcgaccacg atgccattcc attccatcct tggggtttcc
     4681 agcaaccacg cgcccgcggc ggaggcgaca gcggcggcca ggaactgggc tgctgcgcca
     4741 ggcagcaacg gcacccctcg acagaaacgc ccatagtaga gcgtgcccga caccaggccg
     4801 agcacgccga tgaacgccag gatcaggccc tggaatcggg ttgcgctttc caccgccgcg
     4861 tgtccgacga ccaggccgac accgctcatg ccgaggccga ggccgaacca ttggcggggg
     4921 cgcaattgct cgtgcagcag gacgacgccg aggat
//
LOCUS       DASWQN010000905         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1537275, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000905 DASWQN010000000
VERSION     DASWQN010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537275"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..891)
                     /locus_tag="VGC82_15400"
     CDS             complement(187..891)
                     /locus_tag="VGC82_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014416418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HEY0424704.1"
                     /translation="MCETSLYPAVKCFLESRGFDVKGEVKGCDIVAVRRREALTLTIV
                     EMKLGFTLELLLQVTDRMRAANEVWLAVPATRRGRDQDPRVHRLCRLLGFGLMAVRAA
                     RDRVDVLVEPGPYQPRRDHRRRSQLLSEHTRRRGDPSCGGITRQPVMTAYRQQALACA
                     ALLSAGAGRPSELRAMAPDAGKIMLRNVYGWFERTHRGVYRLTIAGEAALRRWPDASV
                     VHAANAAPRPVMPALS"
     gene            complement(1147..1305)
                     /locus_tag="VGC82_15405"
     CDS             complement(1147..1305)
                     /locus_tag="VGC82_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424705.1"
                     /translation="MKKMFLAAFTALGLAATAIAPVAANAAVFHNGSTIAGDAAATRL
                     QQTGTYNQ"
BASE COUNT          298 a          515 c          485 g          301 t
ORIGIN      
        1 attctaaacg taactgaaaa aatctaccag ggtcccagtt acaattcgct tgtcgtattc
       61 cggaggttac gcactggagc agccagcgga tcaccccgtt ggaatttttt gttggtgcat
      121 cccggcgccg gttgaatcga acgggaactg atctgcctgc tgtcccgaac cagtggtgac
      181 ctcgtttcat gagagcgccg gcatgaccgg acgtggcgcc gcattcgcgg catggaccac
      241 gctcgcgtcc ggccaacgcc gcagagccgc ctcaccggcg atggtgagtc gatagacgcc
      301 tcggtgcgtg cgctcgaacc agccatagac attgcgcaac ataatcttgc cggcatccgg
      361 cgccatggca cgcagctcac tgggacggcc cgccccggcg ctcagcaacg ccgcgcaggc
      421 aagggcttgc tggcggtatg cggtcatcac cggctggcgg gttatgccac cgcacgacgg
      481 atcgccccgc cggcgggtgt gctcgctcag caactggctt cgccggcgat gatcccgcct
      541 gggctgatag ggacccggct cgaccagcac gtccacgcga tcacgggcgg cacggaccgc
      601 catgagacca aagccaagga gccggcatag acggtgcacg cgtggatctt ggtcgcggcc
      661 gcgccgggtc gcaggcaccg ccagccacac ttcgttcgcc gcgcgcatcc gatccgtaac
      721 ctgcaacaac aactcaaggg tgaagccgag cttcatctcc acgattgtca gggtcagcgc
      781 ctctcgtcgc cggacagcga cgatgtcgca gcccttgacc tcgcccttca cgtcaaaacc
      841 gcgcgactcc aggaagcact taacggcggg gtaaagtgaa gtttcgcaca tgccgcgcag
      901 cctgctacgg cgtccggcgt cgctgcaacc ccaacatcag cgaaaagaaa atcccttctc
      961 actcccctgt tctggcccag gcggccttct gcatggcttc ggacgataga cttcgccgca
     1021 cctcaacagc aaaaggccgg ggacgacccg gccttcatgc tgtcagttcg gtagcggggt
     1081 taaccgcccc cgtgtcgctt actggccgtc ccgttaactg gccgccttgc ttactggccg
     1141 tctggcttac tggttgtacg tgccggtctg ctgcaaccgg gtggccgctg catccccggc
     1201 aatcgtgctg ccgttatgga agactgcggc attcgcggca accggagcaa tggccgttgc
     1261 ggccaggccc agggcggtga aagcggcaag gaacatcttt ttcatcggaa ctctccatgt
     1321 cggtcgggcg gaatgtccgg ctgcgatgaa tggtgagatg gggcaggtac gccggataat
     1381 aatgctgcat cgcagcattg gagatatgcg ccgagtagcc ggaatatcgc ggtgttgtgt
     1441 gtttccacat cgttgaccgg gggcacggca cccatccagt cacgctccag atgctgtgct
     1501 ccgtcctcgc cgcacgcgcg aacgcttcta acgctgtcaa ctttcctgtc gcgcggcgag
     1561 tgccgctcta tgatagggct acaggatgat ggggctata
//
LOCUS       DASWQN010000906         4277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1537387, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000906 DASWQN010000000
VERSION     DASWQN010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537387"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VGC82_15410"
     CDS             <1..247
                     /locus_tag="VGC82_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0424706.1"
                     /translation="DDALDMGRTLAMDVAILDINLNGQLSYPVAEMLQARGIPFLFAT
                     GYGMAGVPAELRAVPVLSKPFRQQQLAEALIRVIGGS"
     gene            442..888
                     /locus_tag="VGC82_15415"
     CDS             442..888
                     /locus_tag="VGC82_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEY0424707.1"
                     /translation="MTDPFVQPEEPTAAAAIEAISEDLTLFDDWMERYEFIIGMGRKL
                     PPFPDDWANDAHRVPGCQSRVWMEAIPRDGKLYFAGLSDAAIVSGLVALLLRVYSGRS
                     PAEILATDPVFLKDLGLLEALSTNRGNGIAAMARKVREVAAVQQAG"
     gene            896..2059
                     /locus_tag="VGC82_15420"
     CDS             896..2059
                     /locus_tag="VGC82_15420"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424708.1"
                     /translation="MSAATRVAAFLGAYFAANAIASFMPLWFSDRGLSASSIGQILGA
                     AALLRVLAGPCWGTVADRFGQRRPVLVFAALAASGFGLSYATAGTFLPLLLIAAAQGI
                     AASAINPLADSLALALAREGRIEYGPVRAVGSATFMVATAAAGWLLGRIGSWLIPWLL
                     ALSYGASAVLSAFLPEAATPPAATPPAAPRAFAGLMLLRNKAFRLAIASTALIQGAHA
                     AYYGFAALFWRSQGLSDTVIGLLIAEGIIAEILLFARGRRLVERLGPAGLTACAATAS
                     VLRWTITAMAPPLPVLAAVQLVHAATFAMQHLSAMLVLSRFVPADRAATAQALHAALG
                     YGAPAGLMMLLSGWLYAQYGGGLMFMAMAATGGAALILVRPLAQETAESATNR"
     gene            2192..3562
                     /locus_tag="VGC82_15425"
     CDS             2192..3562
                     /locus_tag="VGC82_15425"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0424709.1"
                     /translation="MAVPITRVVVLMMENHSFDRALGWMKQHYPGIEGVDPDHPGMNP
                     DFPVATDTVFQSETRSRNIAKDPGHDLKNILRQIANGNQGFVSDFAQTYPQAGIAERK
                     EIMGYYPYGFLPTLHYLAYNFVVCDHWFSSLPGPTWPNRFFVHSGTSLGHVNMPEGIF
                     NPAVHLYNQRTIYDELSSQRVDWRIYYGDFAQSLLLVHQGQHLDRYRKFDRWEADVAA
                     GDLPSYVFIEPSYFGTAENDQHPPQDIMRGEALIASVFNALHANQALFEQTLLVVLYD
                     EHGGFYDHVSPPSTVPPDGNTRDYAFDRLGVRVPAILISPWLDPGYISTVFDHTSMLR
                     MASQLWPGVQPLGARAAQANSPLDSLKWLPAPRRAIPPAPAPTTLLPIKDLPNLTGQK
                     QAMFNYSLYLESKIDNPAVRTDLMMRSHEAMGGAIAQGNLATDRLSAFIAEKRATPAG
                     TPPTTP"
     gene            3641..4039
                     /locus_tag="VGC82_15430"
     CDS             3641..4039
                     /locus_tag="VGC82_15430"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0424710.1"
                     /translation="MDVFNLHNEARWDPLKHVERVLGKINEGDVTVACWEAGQVSPHH
                     LHPNCTEIYFCLEGGGIMRTPDQTVDIVPGGFVVHPRGELHEYENGPQRTLLFRVRYG
                     DDMSTLIKEWRGNPGWKAKPEDIAHFGLAA"
BASE COUNT          758 a         1412 c         1292 g          815 t
ORIGIN      
        1 ggatgacgcg ctggacatgg gccgcacatt ggcaatggat gtcgcgatcc tggacataaa
       61 cctgaacggg caactcagct atcccgtcgc cgaaatgctg caagcccgcg gtattccctt
      121 cttgttcgcc actggctacg gcatggcggg ggtgccggcg gagctacggg cggtgccggt
      181 gttgtccaaa cctttccgcc agcagcagct cgccgaggca ctgatccgcg tcatcggcgg
      241 ctcgtagccc gatcctgacg ctcgcccgat cctgacgctc gcccgatcct gatgctcgat
      301 aaatatccgg ctgaatcctc ggtcggcatt atccgctgga tccaggcgtt tcacgatccg
      361 ctggatccag gcgtttcacg ccattccaac gtcgcgaact cccgccttga cgtcggcctc
      421 ggctgccagc acatcccgcc catgaccgat ccattcgtcc agcccgaaga acccacggcc
      481 gccgccgcga tcgaggcgat cagcgaagac ctgacgctgt tcgacgactg gatggagcgc
      541 tatgagttca tcatcggcat gggccgcaag ctgccgccat tcccggacga ctgggcgaac
      601 gacgcgcatc gggtccccgg ttgtcaaagc cgggtctgga tggaagccat ccctcgcgac
      661 ggcaaactgt atttcgcggg gctgtcggat gccgccatcg tttccggcct cgtggcattg
      721 ctgctgcggg tctattccgg ccgctcgccc gccgagatcc tggccaccga cccggtcttc
      781 ctgaaggacc tcggcctttt ggaggcgttg tccaccaacc gcggcaacgg catcgccgcg
      841 atggcacgaa aggtgcgcga agtcgccgcc gtgcagcagg ccggataagc agaccgtgtc
      901 ggcggccacg cgggtcgccg cgttcctggg tgcctatttc gccgccaatg caatcgccag
      961 cttcatgccg ttgtggtttt ccgaccgggg cctgtcggca tcgtcgatcg gccagatcct
     1021 gggtgccgcg gcgctgctgc gagtcctggc cgggccgtgc tgggggaccg tggccgatcg
     1081 gttcgggcag cggcgtcccg tactcgtctt cgcggcgctc gccgcttccg ggttcggcct
     1141 atcctatgcc accgccggca ccttcctgcc gctgctgctg atcgccgctg cgcaagggat
     1201 tgccgccagc gccatcaatc ccctggccga ctccctggca ctcgccctgg ctcgggaggg
     1261 ccgcatcgaa tatggcccgg tccgggcggt tggatccgcg acgttcatgg tggcgacggc
     1321 ggcggcggga tggctgctgg gccgcatcgg ctcctggctg atcccctggc tgctggcgct
     1381 gagctacggt gcctcggctg tcctgagcgc gttcctgcca gaggcagcca caccgccggc
     1441 agccacaccg ccggcagcgc cgcgtgcgtt cgccgggctg atgctgctac gaaacaaggc
     1501 gttccggttg gcgatcgcca gcaccgccct gatccaaggt gcgcacgcgg cgtattacgg
     1561 ctttgccgcg ctgttctggc gctcgcaggg tctgagcgac actgtcatcg gcctgctgat
     1621 cgccgaaggg atcatcgccg aaatcctgct gttcgcgcgc ggccgccggc tggtcgaacg
     1681 tttgggtccc gccgggctga ccgcgtgtgc ggcaaccgca tcggtcctgc gctggacgat
     1741 cacggcgatg gcgccgccgt tgccggtgct ggccgcggtg cagctcgtgc atgccgcgac
     1801 cttcgccatg cagcatctgt cggcgatgct ggtccttagc cgcttcgtgc cggcggatcg
     1861 ggcggcgacg gcgcaggcgc tgcacgcagc gctgggatac ggcgccccgg ccgggctgat
     1921 gatgctgctg tcgggctggc tgtatgcaca gtacggcggc ggtttgatgt tcatggcgat
     1981 ggcggctacc ggcggtgccg ccctgatcct tgtgcgccca ttggcgcagg aaacggcgga
     2041 atccgcgacg aaccggtgac gggaattcgt cgaatcgcac gggctattaa cggttattgt
     2101 tggaatccgg tatcccctgt tcgattttgc caagtacccg tatgctgatt ttcaactgaa
     2161 cgaccaaccc agccggcgga ggaagcgaac gatggcggtc ccaataaccc gcgtggtggt
     2221 cctgatgatg gagaatcact ccttcgatcg cgcccttggc tggatgaaac aacattaccc
     2281 cggcatcgaa ggcgtcgatc ccgaccaccc cggcatgaac ccggattttc ccgtcgccac
     2341 cgatacggtt ttccagtccg agacccggtc ccgcaacatc gccaaggacc cgggacacga
     2401 cctgaagaat atcctgcggc agatcgccaa tggaaaccag ggttttgtct ccgatttcgc
     2461 tcaaacctat ccccaggccg ggatcgccga acgcaaggag atcatgggct actatcccta
     2521 cggtttcctg cccacgctac attacctggc ctacaacttc gtggtttgcg atcattggtt
     2581 ttcctcgctt cctggaccca cctggccgaa ccgatttttt gtgcacagcg ggacttccct
     2641 gggtcacgtc aacatgcccg agggcatttt caacccggcg gtacaccttt acaatcaaag
     2701 aacgatatac gacgagctca gtagccagcg cgtggactgg cgcatatact atggtgactt
     2761 cgcgcaatcc ctgctgttgg tgcatcaggg ccagcacctc gatcgttacc ggaaattcga
     2821 ccgctgggaa gccgatgtcg ccgccggcga cctgccgtct tatgtattca tcgaaccctc
     2881 ctatttcggc accgcggaga acgatcagca tccgccacag gacatcatgc ggggcgaggc
     2941 tttgatcgcc agcgtcttca atgccctgca tgccaaccag gcgttgttcg agcaaacgct
     3001 gctggtggtg ctttatgacg aacacggcgg tttctacgat cacgtgtcgc cgccctcgac
     3061 cgtgccgcct gacggcaata ccagggacta tgccttcgat cgcctggggg tcagagtgcc
     3121 ggcgatcctg atctcgccat ggctcgaccc aggctacatc tcgacggtat tcgatcatac
     3181 cagcatgctg cggatggcct cgcagctttg gccgggcgtg caacctcttg gcgcacgcgc
     3241 ggcgcaggca aattcaccgc tcgactcgct caagtggctg ccggctccgc gcagagcgat
     3301 tccacccgcg cctgccccga cgactttatt gccgatcaag gacctgccaa atctgaccgg
     3361 acagaaacag gcgatgttca actatagcct atacttggag agcaagatcg acaatccggc
     3421 ggtacgaacg gatttgatga tgcggtcgca cgaagcgatg ggcggtgcca tcgcccaagg
     3481 aaacctcgcc accgatcgat tgtcggcctt catcgccgag aagcgggcga ccccggccgg
     3541 gacgccccca accacgccct gacgcaagac gagtaatgcc gatgattgat aatcggtgcg
     3601 ggagtgttca agctataccg aacgaaccaa ggaaccggcc atggatgtct tcaatctgca
     3661 caacgaagcg cgttgggacc cgctgaagca cgtcgagcgg gttctcggca agataaacga
     3721 gggcgacgtg acggtagcct gctgggaagc cggacaggtc agtccgcatc acttgcatcc
     3781 gaactgcacc gaaatctatt tctgcctgga aggcggcggc atcatgcgca cgcccgacca
     3841 gaccgtggac atcgtgccgg gcggcttcgt ggtccacccg cgcggcgagc tgcatgaata
     3901 tgagaacgga ccccagcgca cgctattgtt tcgcgtccgc tacggcgacg acatgtcaac
     3961 cttgatcaag gaatggcgcg gcaatcctgg ctggaaggcc aagcccgagg acatcgccca
     4021 tttcgggctg gcggcctagt ggctcgcggt taggctctct cgcctgcgac gacagccgac
     4081 cacgaggctg aaggccccat acctgaagcc gcacgcccac ggaccctcca cacaagccgc
     4141 agccttccca agctcccgat cgtcagcagc gaggcaggac tcccttctcc atgcttccat
     4201 gatctctccg tcctcctcgt caaaagcctt aactaacctc ttcgctctgg cctcaaggag
     4261 gaaaaactgg aactatg
//
LOCUS       DASWQN010000907         3521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_219044, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000907 DASWQN010000000
VERSION     DASWQN010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219044"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1164..1997
                     /locus_tag="VGC82_15435"
     CDS             1164..1997
                     /locus_tag="VGC82_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit II"
                     /protein_id="HEY0424711.1"
                     /translation="MNLLDPQGPVAAANTTILVDSVIIMLAIVVPTIAAILAFAWWFR
                     ASNARARYQPDFVYSGRIELVVWSIPALTVILLGGVAWIGSHQLDPAAPVPGTGTPVR
                     IQAVSLDWKWLFIYPDQRIATVNTLTVPAGAELNFQLTSSSVMNVFFIPQLGSMIYTM
                     NGMVTKLNLRADNEGKLQGLSAHFSGDGFPDMMFDVNVISPLAFPDWVATTAKSDSVL
                     NEDSYRKLMQQAVEKGRPTYRLEDPRLFDMIATQHIPPGPGPQVGSNAGQEHSGEHNA
                     R"
     gene            1987..>3521
                     /locus_tag="VGC82_15440"
     CDS             1987..>3521
                     /locus_tag="VGC82_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0424712.1"
                     /translation="MLGKLDWSAIPLDQPIPLMAGGVVLIAILAVVAWVAVKGHLAYL
                     WHEWITSVDHKRIGVMYILLALVMLLRGGTDAIMMRIQQAIAYHSQGYLPPEHYNQLF
                     SAHGTIMIFFVAMPFVIGLMNLVVPLQLGVRDVAFPTLNSVGFWLTATGALLVNVSLV
                     IGEFARTGWLAFPPLSELSYSPGVGVDYYAWSLQISGVGTLVAGINLVTTVLKLRTRG
                     MSYLRMPMFCWTTLASNLLIVAAFPILTATLAMLLLDRYLGFHFFTNEAGGNVMMFMN
                     LIWAWGHPEVYILVLPAFGIYSEVVATFSGKPLFGYRSMVLATMAICIISFMVWLHHF
                     FTMGAGPDVNAIFGIASMIIAVPTGVKIYNWLFTMYGGRIRFSTPMMWSVGFMVTFII
                     GGLTGVLVAVPPADFLLHNSLFLVAHFHNVIIGGVLFGAFAGIGYWFPKAFGFRLHEG
                     LGKSAFWFTFIGFYVTFVPLYVAGILGMTRRMQHYDVPAWRPWMIVATIGTALLAAGI
                     IFQ"
BASE COUNT          616 a         1077 c         1050 g          778 t
ORIGIN      
        1 tggcggctcg cctgcagacg ttggtcgagc caaatagggc gaagggcaaa tcgtcctgat
       61 ttcaggaccc cggcgggcgc tggccgaggc cgatgaaggg attgcgatca gcgccgacaa
      121 cgaatttgca agtaccgatc acagggcgcg tttcttccgg ggcgccttgt cggcccaggg
      181 ccgcggaaag cctcggcgcc cggatacgcc tatccgagga actcgtagac tagacagcgg
      241 gtccccgcgc gtaaagccgc tcgaaaggat gtcaggaaag gaaatcgtcg gcctgccaat
      301 gccgacggcg ggcttggctg cccagcgcgg cacgctgcac ggcggcgcgg ctcgcatcag
      361 ggagcgtgct gcgcttgcca ccgctacgtc aggcttccaa tccccacagc ttcaggggct
      421 tcgaagaggg atgcggattc gtgcaacaac cgtttgcaaa ccatccgagc tttgaactct
      481 gcccgctaac gaatcgaaaa tcgttttgcc gcacgttcgc gacatgacca cggcaacaat
      541 gggagttcgg agatgagcgg ggatgctgtc atggaaaatg tgcgccgata tcgcgccata
      601 gcttcgttgt accgtcaaac cgcggcccgc cgaccgcatc aaagctgttc tcttctgggg
      661 caagcccagg catgggagca tcgagccgtg tcggagttgg aggcctattt taaggcccgc
      721 gatggctggg cttacgatcc gcgggggcag gtactggcga agtttgccgg gcttggcgcg
      781 ccttcctgac agagatggtg gctggggccg gttgccctcg attgatccat cagaggcaaa
      841 tcaaggactt aacgccggct cagattctca ctttgtgcca ggcaattatg ccgatcccga
      901 gcctttccat tcgcccgggt tgatgcacca gcgccgacgg agtggccttg gttcggtgca
      961 gctaaggcac cgccttcttg acgaacgccg cttcctgcca gaacggccgt tcgtcgtcgc
     1021 tggcaaatag accgcgctgg cggtcttcag ggcaatagct taccattttg cgatcccgtc
     1081 ctgagttgta taaaggaacc atgtcacggg attgaggttt gttgcgaggt ctgtcccgcc
     1141 tctcgattgt cagtggattg acgatgaact tgcttgatcc acaagggccg gttgctgccg
     1201 cgaacacgac tatcctcgtc gactccgtta tcatcatgct cgcgatcgtc gtaccgacga
     1261 tcgctgccat cctggccttc gcgtggtggt ttcgtgcatc gaacgcgcga gcccgctatc
     1321 agccggattt cgtctattcc ggccggatcg agttggtggt gtggtcgatc cccgcgctca
     1381 ccgtcatcct gctcggcggt gtcgcctgga tcggttcgca tcaactcgac cccgccgcgc
     1441 cggtcccggg aaccggcact ccggtccgga tccaagccgt ctcgctcgat tggaaatggc
     1501 tcttcatcta tcccgaccag cgtatcgcca ccgtcaacac gctgaccgtt ccggcgggcg
     1561 ctgagctgaa cttccaactg acctcttcga gcgtgatgaa cgtgttcttc attccgcagc
     1621 tcggcagcat gatctacacc atgaacggaa tggtgacgaa actgaacctt cgcgcggaca
     1681 acgaaggcaa gctccagggt ctctcggcgc atttctccgg cgacggcttt cccgacatga
     1741 tgtttgacgt caacgtgatc tcgccgctcg cctttccgga ctgggtggcc accacggcaa
     1801 aatcagattc tgttctcaat gaagacagct acaggaaact gatgcagcag gcggtcgaga
     1861 agggaaggcc gacctaccgc cttgaggatc cacgcctttt cgacatgatt gcgacccagc
     1921 acattccccc cggaccagga ccacaggtcg gctcgaatgc cgggcaggag cacagcggag
     1981 agcacaatgc tcggtaagct tgattggtct gcgatcccgc ttgaccagcc cattcccctg
     2041 atggccggcg gcgtggtgct gatagcgatt cttgcggtcg tggcctgggt cgccgtgaag
     2101 ggccatctag cctatctctg gcatgagtgg atcaccagcg tcgatcacaa gcggatcggc
     2161 gtcatgtaca tcctgctcgc cttggtgatg ctgctgcgtg ggggcaccga cgccatcatg
     2221 atgcggatcc agcaggcaat cgcctatcat tcgcagggct atctgccacc ggagcactac
     2281 aaccagcttt tctctgccca tggcacgatc atgatcttct tcgtggcgat gccgtttgtg
     2341 atcggattga tgaatcttgt cgtgccgctg caactcgggg tgcgcgatgt cgcctttccc
     2401 acgctcaatt cggtcgggtt ttggctcacg gcgaccggcg cgctgctggt gaacgtctcg
     2461 ctggtcatcg gcgagttcgc gcgtaccggc tggctcgcct ttccgccgct gtcagagctg
     2521 agttattcgc ccggtgtcgg cgtcgactat tatgcctggt cgctgcagat ctcgggcgtg
     2581 ggaacgctcg tcgccggcat taatctcgtc accacggttc tgaagctgcg caccaggggc
     2641 atgagctacc tgcgcatgcc gatgttctgc tggaccaccc tggcctcgaa cctcctgatc
     2701 gtcgcggcgt tcccgatcct gaccgcgacg ctcgcgatgc tgctcctgga ccgctacctc
     2761 ggcttccact tcttcaccaa cgaggccggg ggcaacgtca tgatgttcat gaacctgatc
     2821 tgggcgtggg gtcatccgga ggtgtatatc ctcgtgctgc cggcctttgg catctactcg
     2881 gaggtggtgg cgaccttctc cggaaagccg ctgttcggct accgctcgat ggtgctggcg
     2941 acaatggcga tctgcatcat ctccttcatg gtgtggctgc atcacttctt cacaatgggc
     3001 gccggacccg acgtcaacgc catcttcggc attgccagca tgatcatcgc ggtgcccacc
     3061 ggagtgaaga tctacaactg gctattcacg atgtatggcg gccgcatccg cttcagcacg
     3121 cccatgatgt ggtcggtcgg cttcatggtg accttcatca tcggcggctt gactggcgtt
     3181 ctggtcgccg tcccccctgc ggacttcctg ctgcacaaca gcctgtttct ggtcgcccat
     3241 tttcacaacg tgatcatcgg gggcgttctg ttcggcgcgt ttgccggaat cggctactgg
     3301 tttccgaagg cattcggctt ccggctgcat gaggggctgg gcaagtcggc gttctggttc
     3361 acgttcatcg gcttctatgt cactttcgta ccgctttacg tggcgggaat actcggcatg
     3421 acccggcgca tgcagcatta tgacgtcccc gcgtggcggc catggatgat cgtcgcgaca
     3481 atcggcacgg cactgctcgc ggccggaatt attttccaaa t
//
LOCUS       DASWQN010000908         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_879131, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000908 DASWQN010000000
VERSION     DASWQN010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879131"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VGC82_15445"
     CDS             complement(<1..225)
                     /locus_tag="VGC82_15445"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEY0424713.1"
                     /translation="MKKEDWEIPSNLQPDPSDYSFDLERALKAVVSLKSYVPSDAFTA
                     NTLGTERAGSGVVIKGTGLIATIGYLVTEAE"
     gene            complement(336..884)
                     /locus_tag="VGC82_15450"
     CDS             complement(336..884)
                     /locus_tag="VGC82_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424714.1"
                     /translation="MFPRSRFLVRLGLPMLLLLSVSGCGPARNAFAPVCPTVRLVPAL
                     ADVTRYAGPGPAHDLTDMILQARVVGVDGKCEPGNDTTVLAASVRVLLSVQRGPAMSG
                     REGDLPVFLAVTEGEVVRDKQVYQAHIIFPPNVDRVTITSPEIQMDLPVSPSKSGASY
                     DIIAGFQLSPDELAANRQTTGG"
     gene            927..1229
                     /locus_tag="VGC82_15455"
     CDS             927..1229
                     /locus_tag="VGC82_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424715.1"
                     /translation="MGPAIVPLPNDIGNAAAGPGDGTGGLNLGGWDRGATHSVMAVLG
                     AAIHDFAGTGETVEPAQPRSRRRRRRPCNGHIYGAHGATRASTLLQESPPVTGHPS"
     gene            complement(1226..2302)
                     /locus_tag="VGC82_15460"
     CDS             complement(1226..2302)
                     /locus_tag="VGC82_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEY0424716.1"
                     /translation="MSTPLLFQPISFRSVTARNRIVVSPMCQYSANNGLGNDWHVQHL
                     GARAAGGAGIVFTEATHVSDIGRITPGCLGMYTEEHQALLARLANVITLCGSVPGIQV
                     AHAGRKASSRVPWEGGKPIPVEDGGWVPLAPSPLPLVPGATTPHALSVAEIGNVVSQF
                     AATARMARDAGFKILEIHAAHGYLAHSFLSPVSNQRNDSYGGDLSGRSRFLMETIEAV
                     RDEWPSDLPLWVRLSCSDWMPGGLTIEDTVAVSRNLAATGLVDLIDCSSGGVSHEQKI
                     PSLYPGYQVPFADAIRNRAGIATGAVGMITEPTHAAEIVANGRADVVLLARALLADPA
                     WPLRAAKALGVKPEVPPQYLRAAL"
     gene            2397..3257
                     /gene="pdxY"
                     /locus_tag="VGC82_15465"
     CDS             2397..3257
                     /gene="pdxY"
                     /locus_tag="VGC82_15465"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HEY0424717.1"
                     /translation="MNILSIQSWVAYGHVGNASAVFPLQLLGAEVWAINTVQFSNHTG
                     YGDWKGDVYSGQSVRDLVDGIAARGALKRCDAVLTGYVGGADIGDAILHAVASVRRAN
                     PGSLYCCDPVIGDIDTGVYVRPGIEEFLQRHALPAADIATPNQFEIERLTGLDCSSLH
                     GAKQAVLRLRSMLHSGGPACVLVTSLKTDGTPDDCIDMMTFENGAFHLLRTPRLGVTV
                     NGAGDAIAALFLFHRMRSGSAVAALEAAGSSVFGLLRRTEEAGSREILMVQAQNEFVA
                     PTRMFRAMAV"
     gene            complement(3400..>3548)
                     /locus_tag="VGC82_15470"
     CDS             complement(3400..>3548)
                     /locus_tag="VGC82_15470"
                     /inference="COORDINATES: protein motif:HMM:NF022782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3140 domain-containing protein"
                     /protein_id="HEY0424718.1"
                     /translation="SDLTDDDVADMRKVIGYVHRHLAQRPQGDVSDTRWRKSLMNWGH
                     DPVA"
BASE COUNT          633 a         1089 c         1224 g          602 t
ORIGIN      
        1 ctccgcctcg gtcaccagat agccgatcgt ggcgatcagg cctgtaccct tgatcaccac
       61 cccgctgccc gcccgctcgg ttccgagcgt attcgcggtg aacgcgtccg agggaacata
      121 ggatttcagg ctaacaacgg ccttcagcgc ccgttcgagg tcgaacgagt agtcgctggg
      181 gtcgggttgc agattggacg ggatctccca gtcttccttc ttcatacctc gacgtcttcc
      241 ctggttcggg cggcccttcg gtggcgtatc ggtcataatc tgattatatc ggatcttttg
      301 gcgcagccga caatggcggc ggctgcaaat gaagttcaac cgcccgtcgt ttggcgattc
      361 gctgccagtt cgtccgggga aagctggaat ccggcgatta tgtcatagga cgcgcccgat
      421 ttggaaggtg aaaccggcaa gtccatctgg atctctgggc tggtaatggt cacccgatcg
      481 acgttcggcg gaaatatgat gtgcgcctga tacacctgct tgtcgcgaac gacctcgccc
      541 tcggtcaccg cgaggaacac cggaaggtct ccctcacggc cgctcatggc cggcccccgc
      601 tgaaccgata gaagaacgcg cacgctggct gccagaaccg tcgtgtcatt accgggttcg
      661 cacttgccat cgaccccgac cacgcgggcc tgcaggatca tgtcggtcag gtcatgcgcc
      721 ggaccgggac cggcatagcg cgtcacgtcc gccagcgccg gaaccaggcg aaccgttgga
      781 cagacgggag caaacgcgtt gcgcgcagga ccgcatccgg acacggacaa cagcagcaac
      841 atcgggaggc caaggcggac aagaaagcgc gaacgaggga acatcaatga ctccggaggc
      901 cggaaagata tccggcttct cccgctatgg ggccggccat tgtgccgctg ccgaacgaca
      961 tcggcaatgc ggctgcggga ccgggggatg ggaccggggg attgaacctg gggggatggg
     1021 accggggcgc gactcacagt gtcatggccg tgcttggcgc ggccatccac gactttgccg
     1081 gcacggggga aaccgtggag ccggcacagc cccggtcacg acgcaggcgg cgaagaccgt
     1141 gcaacggcca catctatggc gcacatgggg cgacgcgcgc ctcgacgctg ctgcaggaat
     1201 caccgccggt tactggtcat ccctcctaaa gcgccgcgcg caaatactgc ggcgggacct
     1261 cgggcttcac gcccagagcc ttggcggcac gaagtggcca tgccggatcg gccagcaacg
     1321 cccgagccag caggactaca tccgcccggc cgttcgcgac gatctccgcc gcatgggtcg
     1381 gttcggtaat catgccgacc gcgccggtgg cgatgcccgc ccggttgcgg atcgcgtcgg
     1441 cgaaagggac ctggtaaccg ggatacagcg acgggatttt ttgctcgtgc gatacgccgc
     1501 cggacgagca gtcgatcagg tccaccaatc ccgtcgcggc gagattgcgg gaaacggcga
     1561 cggtgtcctc gatggtcaat ccgcccggca tccagtcgct gcacgacagg cgcacccaaa
     1621 gcggcagatc ggagggccat tcgtcgcgga ccgcttcgat cgtctccatc aggaagcggg
     1681 agcggccact aaggtcgccg ccataggagt cgttgcgctg gttagaaacg ggcgacagga
     1741 aggaatgcgc caggtagccg tgcgcggcat ggatctccag gatcttgaag ccggcatcgc
     1801 gggccatccg ggcggtggcg gcgaattggc tgacgacgtt tccgatctcc gccacggaca
     1861 aggcatgtgg ggtggtcgca ccgggaacca acggcagcgg gctgggcgcg agggggaccc
     1921 acccgccatc ctcgacgggg atcggcttgc cgccttccca gggaacgcgg gaggacgcct
     1981 tgcggccggc gtgggcgacc tggatgcccg gcaccgaacc gcacagggtg atgacgttgg
     2041 ccaggcgggc gagcagcgcc tggtgttctt ccgtgtacat gccaagacag ccaggggtga
     2101 tgcggccgat gtcggagaca tgcgtagcct cggtaaacac gatccccgcg ccgccggcgg
     2161 cccgggcgcc gaggtgctgg acgtgccaat cgttgccgag gccattgttg gcgctgtact
     2221 ggcacatcgg cgacacgacg atgcggttgc gggcggtcac cgagcggaag ctgatcggtt
     2281 gaaaaagcag cggcgtggac atgcggcggg ggttcctgtg aagccggtgc gcggagctcc
     2341 ggatgcgtta cggccgcata tagcgtattg cccatgcgaa ggaaccgggg gcccgcgtga
     2401 acatcctgtc gatccagtcc tgggtggctt atggccatgt cggcaatgcc agtgcggtgt
     2461 tcccgctgca gcttctggga gccgaggtct gggccatcaa caccgtccag ttctccaacc
     2521 acactggata cggtgattgg aaaggggacg tgtattccgg ccaatcggtg cgggatctgg
     2581 tggatgggat cgcggccagg ggtgcgctga agcggtgcga cgcggtgctg accgggtatg
     2641 tcggcggtgc ggatatcggc gatgccatcc tgcatgcggt ggcatcggtc aggcgagcca
     2701 atcccggctc gctctattgt tgcgatccgg tgatcggcga catcgacacg ggggtctatg
     2761 tccggccggg tatcgaggaa ttcctgcaac ggcatgcttt gccggcagcg gatatcgcca
     2821 cgccgaacca gttcgagatc gagcgcttga ccgggctgga ctgctcgtcg ctgcacggcg
     2881 ccaagcaggc tgtgctgcgg ctgcggtcga tgctgcactc cggcggtccg gcctgcgtgc
     2941 tggtgaccag tctgaagacg gacggcacgc cggatgattg catcgacatg atgacgttcg
     3001 agaacggagc gtttcacttg ctgcgcacgc ccaggctggg ggtgacggtc aacggcgcag
     3061 gggatgcgat cgcggcattg ttcttgttcc accggatgcg cagcggcagc gcggtggccg
     3121 cactcgaggc agcgggttcc tcggtgttcg ggctgctgcg ccggaccgag gaagccggct
     3181 cgcgggagat cctgatggtc caggcgcaaa acgaatttgt cgcgccgaca cggatgttcc
     3241 gggcgatggc agtataggat tggacgccgc ggcagccggc ttcaacctgc cgcgctcagg
     3301 cgagggccat cctcaagcgg cgggccatcc cagacggcgg gccatcccag acggcggccc
     3361 atcccaggcg gcgggccgtc ttcaagcgat tggccaccct caagcgacag gatcgtgtcc
     3421 ccaattcatc aatgacttgc gccagcgcgt atcggacacg tcgccttgtg gacgttgcgc
     3481 gagatggcgg tggacatagc cgatgacctt gcgcatgtcg gcgacatcgt cgtccgtaag
     3541 gtcgctcc
//
LOCUS       DASWQN010000909         8470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1010447, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000909 DASWQN010000000
VERSION     DASWQN010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 8470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8470
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010447"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /gene="ppsR"
                     /locus_tag="VGC82_15475"
     CDS             <1..767
                     /gene="ppsR"
                     /locus_tag="VGC82_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010109 - regulation of photosynthesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator PpsR"
                     /protein_id="HEY0424719.1"
                     /translation="RASGRADDVHAKLLDGEQEVVVTASLFRDEGGTACLVRIAQPAG
                     EPASGAAPKLKAKLLKLMESAPDAFVVTGADGRIITANATFLELAQLPTEEQARGELL
                     TRWIGRSGVDLDILIANLRQHGSVRLFASLLRGELGEPAEVEISAVTVMNGGLPCFGF
                     AIRDIGRRLQREPRIERALPRSLEQLTELIGRVALKDLVREATDVIERLSIEAALNLT
                     GDNRASAAEMLGLSRQSLYVKLRRYGLGDPEAAGAD"
     gene            778..1701
                     /gene="chlG"
                     /locus_tag="VGC82_15480"
     CDS             778..1701
                     /gene="chlG"
                     /locus_tag="VGC82_15480"
                     /EC_number="2.5.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyll synthase ChlG"
                     /protein_id="HEY0424720.1"
                     /translation="METAPAFAVTEARPVSSRPPSSVAAMLELLKPITWFAPMWAFGC
                     GVVSSGVPLGERWWALILGVILAGPMVCGTSQAVNDWFDRHVDAINEPNRPIPSGRLP
                     GRQGLYIAIVWTLLSLLIAAPLGRWGFGAAVVGLGLAWAYSAPPFRLKRNGWWGNATC
                     AACYEGLPWVTGAAVMAATGPGWRILAIAALYSAGAHGIMTLNDFKSVDGDRQTGIFS
                     LPVLLGTEAAANAACIAMTLPQIGVVALLLSWGFVTFAFAVTLLLMAQLLLMVRLMRE
                     PRANAPWYNATGTTLYVIGMLITAFALRITS"
     gene            1718..2920
                     /locus_tag="VGC82_15485"
     CDS             1718..2920
                     /locus_tag="VGC82_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl diphosphate reductase"
                     /protein_id="HEY0424721.1"
                     /translation="MNETYDAVVVGGGPCGATAAGELARKGWTVLLLDRAGRIKPCGG
                     AIPPRLIQDFGIPDHLLSARITSARMVAPSARSVDMPIDNGFVGMVDRETFDEWLRAR
                     AAADGATRRTGKFTKITRDQDGMALVHYQSKGEDTSCIRAKVVLGADGAKSSVAGQCI
                     PGADKIPHVFAYHEIVRIPAPGPDGTCFDGTRFDGARCDVYYQGRLSPDFYGWVFPHG
                     DTASVGSGSMRKGFSLRRSVTDLRQAAGLAGAETIRREGAPIPLHPLRRWDNGRDVLV
                     AGDAAGVVAPASGEGIYYAMEGGRLAAAAAGEFLATGDARALRTARRRFMKAHGRVFW
                     ILRMMQWFWYSSDKRRERFVSIWQDPDVQRLTWDSYMNKRLTRGNPAAQVRIFFKDLA
                     HLLGLASA"
     gene            2920..3390
                     /locus_tag="VGC82_15490"
     CDS             2920..3390
                     /locus_tag="VGC82_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEY0424722.1"
                     /translation="MPEIIGAAAWGIIVAASGAFLTDISPWYRSLHKPSWQPPDWLFG
                     PAWTLILGLASLSAYFAWRSATDSGSRMLIAVLFLLNGIANILWSPLFFKLRRPDWAL
                     WEVPFLWLSILAPIVLLLPISRVASLLLVPYLAWVSFAAFLNLTIVRLNQPFGG"
     gene            3443..4312
                     /locus_tag="VGC82_15495"
     CDS             3443..4312
                     /locus_tag="VGC82_15495"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-polyphosphate phosphotransferase"
                     /protein_id="HEY0424723.1"
                     /translation="MKIDTNDFRVREGGKIDVEKRPTDIDPVYKSKEQYKQLLAEHVE
                     ALSAQQQLLYASNRYAVLLIFQAMDAAGKDGVIRHVMSGVNPQGCQVFSFKHPSATEL
                     QHDFLWRTTRDLPERGRIGIFNRSYYEEVLVARVHPDILRNESLPDVPLDDKSIWASR
                     YRSIVDLERHLHDNGTRIVKFFLHLSKEEQRKRFLARIDEPRKNWKFSLADIEERQYW
                     KQYMKAYGKCLAATSTRYAPWYIVPADDKENARLIVSRIILDTLEALKMSYPETSPDR
                     LRELSSIREKLAP"
     gene            complement(4358..4828)
                     /locus_tag="VGC82_15500"
     CDS             complement(4358..4828)
                     /locus_tag="VGC82_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424724.1"
                     /translation="MTTPDAFALRNSGLNAFLFAEVGTELNGSPLTVLSVLARLGQDP
                     WAEAAKWAKLPRAAMIDRLAQSIAQMPLSPEALTDARSTASRLAQLLPSQVVRPSQTP
                     IRSKASVPRWVMAAICCAAVALGLAFNFNLGSSSSKTVTPISDLPARKPPAPSH"
     gene            4983..5537
                     /locus_tag="VGC82_15505"
     CDS             4983..5537
                     /locus_tag="VGC82_15505"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0424725.1"
                     /translation="MTDPRLERIAGLLTLKAGGQYGLSDINQRQHALQAAWLAEKMGC
                     PDSVIVAGLLHDVGHLVHDLGDNPAEDGVDDLHEQRGYDFLRVWFAPEVTEPVRLHVA
                     AKRYLCAREADYFAKLSKDSVLSLSLQGGPMSADEVAAFEAVPQSAAAVQLRRFDEQA
                     KVRDLETPPVGHFLPYVGRCLLPA"
     gene            complement(5704..6819)
                     /locus_tag="VGC82_15510"
     CDS             complement(5704..6819)
                     /locus_tag="VGC82_15510"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY0424726.1"
                     /translation="MKITGVETYWTKTPFDMGGKPAVVGGLNWQTMNTVWLRITTDQG
                     IEGWGEAFGHATAAATMAVLDTQLAPAILGQDARDIAGLRQRLAKAFHTFGRNGPHVF
                     ALSALDIALWDIAGKAANQPLWRLLGGSPTETLTCYASLLRYGEAAQVAAACERAGSR
                     GYRDVKLHETTTPEIAAARDALGDEARLMVDVNCPWTVWQALEMARALEDLDLTWLEE
                     PVWPPGDHEGLSRVRLEGGIPVAAGENAAGLHDFRAAFEAGAQDIAQPSVIKIGGPSA
                     MVEIAALAKAFGVRLVPHNAYFGAGYLASLHVNAALAPDAPFERLFVDLEASPYHELV
                     QAKDGKVMVPTGPGLGRDPDMDVLLRYLVREPGKHTG"
     gene            6942..>8470
                     /locus_tag="VGC82_15515"
     CDS             6942..>8470
                     /locus_tag="VGC82_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY0424727.1"
                     /translation="MTARIRLAVDIGGTFTDLVLSLPDRTVSSKLLTTPGAPDDAVIA
                     GTRAILAEAGIDGAAVNLVIHGTTLATNALIERKGARTALITTAGFRDSLEIAYEHRF
                     EQYDLYMERPTPLVERDLRLEVPERLAADGSVLLPLNEAALLALVDVLQTERIEALAI
                     SFLHSYINPAHEERARDLLTAAMPGLAITLSSEVCRGIREYERTSTTVANAYVLPLMA
                     SYLGRMRSGLTGIGVTCPLLLMMSSGGICTVETATRFPVRLIESGPAGGVILARRIAA
                     LGGYDRALSFDMGGTTAKITLIDDFTPQQSRHFEVARTYRFAKGIGIPVRIPVIDMVE
                     IGAGGGSIGRVDQLRRIAVGPDSAGSDPGPACYGRGGSAATVTDADVVLGRIDPGRFA
                     GGKIVLDTVRARDAVFAGVGSRLGMPVEDAARGIVEIVDETMASAARVHAVENGKDTA
                     SRTLIAFGGAAPLHAVRLAQKLGISRVVIPVDAGVGSAHGFLDAPIAYEVARTFLVRL
                     D"
BASE COUNT         1486 a         2775 c         2661 g         1548 t
ORIGIN      
        1 ttcgcgcgtc cggccgagcg gacgatgtgc atgcgaagct gcttgatggc gagcaggagg
       61 tcgtggtgac cgcctcgctg ttccgcgacg aaggcggcac cgcctgtttg gtgcgcatcg
      121 cccagcccgc cggcgaaccg gcttccggag ccgcgccgaa gctgaaagcc aagctgttga
      181 aactgatgga aagcgcaccg gacgctttcg tcgtaaccgg tgctgacgga cggataatca
      241 ccgccaacgc gaccttcctg gaactcgcgc aactgcccac tgaggagcag gcccggggcg
      301 agttgttgac ccgttggatc ggtcggtcgg gggtcgatct cgacatcttg atcgccaacc
      361 tgcgccaaca tgggtccgtg aggctcttcg ccagcctgct gcgcggcgaa cttggcgaac
      421 cggccgaggt ggagatatcc gcggttaccg tcatgaacgg cgggctgccg tgcttcggct
      481 tcgccatccg cgacattggc cggcggctac agcgtgaacc tcgcatcgag cgtgccctgc
      541 cacgctcgct ggagcaactg accgaactca ttggcagagt cgcgctgaag gatctggtgc
      601 gcgaagcaac cgatgtgatc gagcgcttgt ccattgaagc cgcgctcaac ctcactggcg
      661 acaaccgagc ctctgctgcc gagatgctcg ggctaagccg ccagagcctt tacgtgaagc
      721 tacgccgcta cggcctcggc gatcccgaag ccgcgggcgc ggattgaagg agcccgcatg
      781 gaaaccgccc ccgcgtttgc cgtgacagaa gcccggcctg tttcaagccg accaccttcg
      841 tcggttgccg cgatgctgga attgctgaag ccgataacat ggttcgcgcc gatgtgggcc
      901 ttcggctgcg gcgtggtgtc atccggagtg ccgcttggag agcgttggtg ggcgctgatt
      961 ctgggggtga tcctggccgg cccaatggtg tgcggcacca gccaggcggt gaacgactgg
     1021 ttcgaccgac atgtggatgc gatcaatgaa cccaaccgcc ccatcccgtc cggccgcctg
     1081 ccggggcgcc aaggcctgta tatcgcaatc gtctggacac tgctgtcgct gctgatcgcc
     1141 gcacctttag gtcgctgggg gtttggcgcc gcggtggtcg gcctgggtct ggcctgggca
     1201 tacagcgcgc cgcctttccg gctgaagcgg aacggctggt ggggcaacgc cacctgcgcc
     1261 gcctgctacg aagggttgcc ctgggtaacc ggcgcggcgg tgatggcggc caccgggccg
     1321 ggctggcgca tccttgccat cgccgcgttg tacagcgccg gcgcgcatgg catcatgacc
     1381 ttgaacgact tcaagtccgt cgatggcgac cggcagacgg gaattttctc gctgcccgta
     1441 ctgcttggga ccgaggcagc ggccaacgcc gcctgtattg ccatgacctt gccgcagatt
     1501 ggcgtcgtgg cattgctgct ctcgtggggt ttcgtgacgt tcgccttcgc cgtgacgctc
     1561 ttgctgatgg cccaactgct gctgatggtc cgcctgatgc gcgaaccgcg ggcgaacgcc
     1621 ccgtggtaca acgccaccgg caccaccctc tatgtcatcg gcatgttgat aaccgcgttc
     1681 gcgctcagga tcacatcatg acgccagggg tcgcacgatg aacgaaacat acgatgccgt
     1741 ggtggttggc ggcggtccat gcggcgcgac ggccgccggc gaactggccc gcaagggctg
     1801 gacggtgctg ctgctggatc gtgccggccg gataaagccc tgcggcggtg cgataccgcc
     1861 caggctgatc caggattttg gcattcccga tcacctgctg tcagcccgca tcacgtccgc
     1921 ccgcatggtg gcaccctccg cccggtcagt ggacatgccg atcgataacg gctttgtcgg
     1981 catggtcgat cgcgaaactt tcgacgaatg gctgcgagcg cgcgcggctg cggacggcgc
     2041 gacccgccgc accggaaagt tcacgaagat cacgcgcgac caggatggca tggcgttggt
     2101 gcattatcag tctaaaggcg aggatacctc ctgtatccgc gccaaagtcg ttcttggtgc
     2161 tgacggcgcg aaatcctcgg tcgcgggcca gtgcataccc ggcgccgaca agatcccgca
     2221 cgtcttcgcc taccacgaga tcgtccgcat tcccgccccc ggccctgatg gcacctgctt
     2281 cgatggcacc cgcttcgatg gcgcccgttg cgacgtgtac tatcaaggcc ggctgtcgcc
     2341 cgacttctac ggctgggtct ttccgcatgg cgacacggcc agcgtcggca gcggatcgat
     2401 gcggaaaggc ttctcgctgc gccggtcggt caccgatctg cgccaggccg ccggtctggc
     2461 gggtgccgag accatccgcc gcgaaggcgc cccgatcccg ctgcacccgt tgcgccgctg
     2521 ggacaatggc cgcgacgtgc tggtggcagg cgatgcggcg ggggtggttg cgccggcctc
     2581 gggcgagggc atctactacg ctatggaagg cggccgcctg gctgccgccg cagccggcga
     2641 atttctcgcc acgggtgatg ccagggcact acgaaccgcg cgccggcgct tcatgaaggc
     2701 gcatggcaga gtgttctgga ttctgcgtat gatgcagtgg ttctggtact ccagcgacaa
     2761 gcggcgggaa cgcttcgtca gcatctggca ggaccccgac gtgcaacgcc tcacctggga
     2821 ctcctacatg aacaagcgcc tgacgcgtgg caacccagcc gcccaagtgc ggatcttctt
     2881 caaggatctc gcacacctgc tcggcctggc ttcggcgtag tgcccgaaat catcggagcg
     2941 gctgcttggg ggattatcgt ggccgccagc ggcgcttttt tgacggatat ttcgccctgg
     3001 taccgctcgc tccacaaacc atcctggcag ccccccgact ggttgttcgg gccggcctgg
     3061 acgttgatcc tggggctggc ctcgctgtcg gcctacttcg cgtggcgcag cgccactgat
     3121 tccggcagcc ggatgttgat cgcggtgctg ttcctgctga acggaatcgc gaatatcctc
     3181 tggagcccgc tgttcttcaa gctgcgccgg cccgactggg cactgtggga agtaccgttc
     3241 ctgtggctgt cgattttggc gccgatcgtg ctgctgctgc cgatttcccg tgtcgcgagc
     3301 ctgctgctgg taccttacct ggcctgggtc tccttcgccg cgttcctgaa tctgacgatt
     3361 gtccggctta accagccctt cggaggctga taccaagggg ttcggcccat agtggcaatc
     3421 ggcccatagt ggaaaagcga ccatgaaaat cgataccaac gacttccgcg tgcgcgaagg
     3481 cggcaagatc gacgtcgaaa aacggccgac ggacatcgat ccggtttaca agtcgaaaga
     3541 acaatataag cagcttctgg cggagcacgt cgaggcgctc agcgcgcagc agcagcttct
     3601 ctatgcgtca aaccgctatg ccgtcctgct gatcttccag gcgatggacg cggccggaaa
     3661 ggatggcgtc atccggcacg tcatgtccgg cgtcaatccg cagggctgtc aggtgttcag
     3721 tttcaaacac ccaagcgcca ccgaactgca gcatgatttt ctgtggcgca cgacccgcga
     3781 tctgccggag cggggacgaa tcggcatctt caaccggtcc tattacgaag aggtcctggt
     3841 cgcacgggtc catcccgaca tcctccgcaa cgaaagcctg ccggacgttc ctctggacga
     3901 caaatccatc tgggccagcc gttaccgctc gatagtcgat ctggaacgcc acctgcacga
     3961 caacggcacg cgtatcgtca agttcttcct gcatctttcg aaagaggaac agcggaagcg
     4021 tttcctcgca cgcatcgacg agccgcgaaa gaactggaaa ttcagcttag ccgacatcga
     4081 ggaacgccag tactggaaac aatacatgaa ggcctatggc aaatgcctgg ccgccaccag
     4141 tactcgctat gccccctggt atatcgtgcc tgccgacgac aaggaaaatg cccgtttaat
     4201 tgtgtctcgc atcattctgg acacgctgga agcactgaaa atgagctatc cggaaacgag
     4261 cccggaccgc ctccgcgaac tttcctcgat tcgggaaaag cttgcgccat agatttcagg
     4321 ctgaaattcc tcggctggga aagccgcaac tgacctatca gtgcgagggc gccgggggct
     4381 ttcgggccgg caaatcgctg atcggcgtga ccgtcttgct ggatgacgaa cccagattga
     4441 aattgaatgc caaacccagt gccacggccg cgcaacaaat cgccgccatc acccatcgtg
     4501 gcacggacgc cttgcttctg atcggagttt ggcttggacg aacaacctgc gacggcagca
     4561 actgagcaag ccgcgacgcg gtactgcggg catccgtcag cgcctcgggc gatagcggca
     4621 tctgcgcgat actctgcgcg agccgatcga tcatcgcggc cctaggcagc tttgcccact
     4681 tcgcggcctc ggcccaagga tcctggccca agcgcgccag aaccgacaga accgtcagcg
     4741 gcgatccatt cagttcagtt ccaacctcgg cgaacaggaa tgcgttcagg cctgagttcc
     4801 gcaaagcgaa ggcgtccggg gtcgtcatgc tgatcccact ccttattgcc atatagatga
     4861 tatgcgccat tcgccgctgc aagtcgtcaa ccccgtcttt gaattttcgg cgaagcggtc
     4921 gttgctgccc gctaaacgag ccggtatcct gcctgcacaa agcggtggca gaggacgcga
     4981 cgatgaccga cccaagactc gagcggattg ccgggttgct caccctgaaa gccggcggcc
     5041 aatacggact gtccgacatc aaccaacgcc agcacgcttt acaagcggct tggctggcgg
     5101 agaagatggg gtgcccggac tcggtgatcg tggccggcct gctgcacgat gttgggcacc
     5161 tggtgcacga tctgggtgac aacccggctg aagacggcgt ggacgacctg cacgaacagc
     5221 gcggctacga cttcctgcga gtgtggtttg cacccgaagt caccgaaccg gtgcgcctgc
     5281 acgtcgccgc taaacgctac ctctgcgcaa gagaagccga ctatttcgcc aagctgtcga
     5341 aggactcggt gctgagcctg tcgctgcaag gcggcccgat gtcggcggac gaggtcgccg
     5401 ccttcgaggc ggtgccgcag tccgccgctg ccgtgcagct ccgccggttc gatgagcaag
     5461 cgaaggtcag ggacctggaa acgccgccgg tggggcattt ccttccttat gtcggacgtt
     5521 gcctgctgcc ggcctgagcc ctccggtcgc catgcgatgc tgaggcgttg acaaataaac
     5581 gtttcgaggc gggagacgca gaccatgtct tgggcggcct gtgacagcaa cccaggacct
     5641 tcccgcgttc aggcaaagtc gtgggtggtc gcgccaagca tagccatgac aattaggcga
     5701 cagtcagccg gtatgcttac ccggctctcg caccagatac cgcagcagca cgtccatgtc
     5761 ggggtcgcgt cccagtcccg gcccggtcgg caccatcacc ttgccatctt tcgcctgcac
     5821 cagctcgtga taggggctgg cttccagatc gacaaacagc cgttcgaacg gcgcgtccgg
     5881 cgccaatgcg gcattgacgt gcagcgacgc cagatagccc gcgccaaaat acgcattatg
     5941 cggcaccaga cggacaccga acgccttggc cagcgccgca atctccacca tcgccgaggg
     6001 cccgccgatc ttgatcacgc ttggttgggc gatgtcctgc gctccggcct cgaacgcggc
     6061 gcgaaaatca tgcagtccgg cggcattctc cccggcagcg accggaatcc cgccctccaa
     6121 tcgaacccgg gacagaccct catgatcgcc gggcggccac acgggttctt ccagccaggt
     6181 cagatcgagg tcttccaacg cccgcgccat ttccagcgcc tgccagacgg tccatgggca
     6241 gttcacgtcc accatcagcc tggcctcatc accgagtgca tcgcgggcgg cggcgatttc
     6301 gggagtggtg gtctcgtgga gcttgacgtc gcggtagcct cgggatccag cccgttcgca
     6361 ggcggcggca acctgagcgg cctcgccata gcgcagcagg ctggcgtagc aggtcagcgt
     6421 ctcggtcggt gatccaccga gcaggcgcca caacggctgg ttggccgctt tgccggcgat
     6481 gtcccatagc gcgatgtcca gtgccgatag cgcaaagaca tgcgggccat tgcggccaaa
     6541 ggtatggaac gcttttgcca ggcgctgccg cagcccggcg atgtcgcgcg cgtcctgccc
     6601 caggatcgcc ggcgcaagct gggtatccag caccgccatg gtcgcggcgg ccgtagcgtg
     6661 cccaaacgcc tcgccccatc cctcgatgcc ctgatccgtc gtgattctca gccaaaccgt
     6721 attcatggtc tgccagttca gcccgcccac gaccgccggc ttgccaccca tgtcgaaggg
     6781 cgttttggtc cagtaggttt cgaccccggt gatcttcacg gcgttctccc tcttccgtcc
     6841 caccgactct gcctcggggc cggccgaaat gccagccggg cgccgcaccc tggacgaaac
     6901 cctacgaacc gtggattctt tccgcacgaa aggatgccac catgaccgcc cgaattcgtc
     6961 tcgccgtgga catcggcggc accttcaccg acctcgtgct gtctttgccg gaccggaccg
     7021 tctccagcaa attgctgacc acacccggcg caccggacga cgcggtcatc gccggcacac
     7081 gcgcgattct ggccgaggcc gggatcgacg gcgccgcggt gaacctggtc atccacggca
     7141 cgaccctggc caccaacgcg ttgatcgaac gcaagggcgc caggacggcg ttgatcacga
     7201 ctgccggctt ccgcgactcg ctggagatcg cttacgagca ccgcttcgaa caatacgatc
     7261 tgtatatgga acgccccacg ccgctggtgg aacgcgacct gcggctggaa gtcccggaac
     7321 gtctggcagc cgacggctcg gtcttgctgc cgttaaacga ggctgcgctg ctggccctgg
     7381 tcgatgtcct gcaaaccgaa cggatcgagg cgctcgcgat cagcttcctg cattcctaca
     7441 tcaatcccgc gcacgaggaa cgcgcccgcg accttctgac cgccgcaatg cccggtctgg
     7501 ccatcaccct gtcctctgag gtgtgccgcg gaattcgcga atacgagcgg acatccacca
     7561 cggtcgccaa tgcctacgtg ctgccgctta tggccagtta tctcggccgc atgcggtctg
     7621 gcctgactgg catcggcgtg acctgtccgc tgctcctgat gatgtcgtcc ggcggaattt
     7681 gcacggtcga gacggcaacc cggttccccg tgcgactcat cgaaagcggg ccggccgggg
     7741 gcgtgatcct ggcgcggcgc atcgccgccc tcggcggata cgatcgcgcc ctgtcgttcg
     7801 acatgggcgg gaccaccgcg aaaatcacct tgatcgacga cttcacgcca cagcaatcga
     7861 ggcatttcga ggtcgcccgg acctaccgtt tcgccaaggg catcggcatc ccggttcgca
     7921 tcccggtgat cgacatggtg gagatcggag ccggcggcgg ctccatcggc cgggtggatc
     7981 agttgcgccg aatcgcggta gggccggaca gcgccgggtc tgatcccggg ccggcgtgct
     8041 atggccgtgg cggttcggcg gccaccgtga ccgatgcgga tgtggtgctc ggccggatcg
     8101 acccggggcg gttcgccggc ggcaagatcg tactggatac ggttcgcgcc cgggacgccg
     8161 tgttcgccgg cgtcggctcc cgcctgggca tgccggtgga agacgccgcg aggggcatcg
     8221 tcgagatcgt ggacgaaacc atggccagcg cagcacgcgt tcatgcggtg gaaaacggca
     8281 aggataccgc gagccggacg ttgatcgcct ttggcggcgc cgcgccatta catgccgtcc
     8341 gcctggcaca gaaactcggg atctcgcgcg tcgtgatccc ggtggatgcg ggcgtcggtt
     8401 ccgcccacgg ctttctggat gcgcccatcg cctacgaggt cgcccggacg ttccttgtgc
     8461 ggctcgattt
//
LOCUS       DASWQN010000910         3868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_87340, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000910 DASWQN010000000
VERSION     DASWQN010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87340"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /gene="wrbA"
                     /locus_tag="VGC82_15520"
     CDS             <1..433
                     /gene="wrbA"
                     /locus_tag="VGC82_15520"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEY0424728.1"
                     /translation="SAPVAVVADLANYDAIVVGTGTRFGRMSSQMANFLDQAGGLWAK
                     GALNGKVGAAFSSSATQHGGQETTLFSIITNLLHFGMVVVGMDYGFAPQMTLEEVVGG
                     SPYGATTIAGGDGSRQPSQKELDGARYQGRRVAEVAAKLHG"
     gene            complement(472..1260)
                     /locus_tag="VGC82_15525"
     CDS             complement(472..1260)
                     /locus_tag="VGC82_15525"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEY0424729.1"
                     /translation="MIIEDRRFLASQIAFVASPTPSAHEARENLIRRYGECPLPQASV
                     VVALGGDGFMLETIHQVMPLNVPVYGMNRGSVGFLMNTYSEDDLPNRLSRAQAAELHP
                     LRMHVITATGSVEEAVAFNEVSLLRQLRQASKIRISVDGRVRLEELSCDGVLISTPAG
                     STAYNLSAHGPIVPLSANLLPLTPISAFRPRRWRGALLPSSADVLFEVLEADKRPTAA
                     VADFTEVRDVVSVAVSEDRSVTVTVLFDPDRGLSERIIAEQFTV"
     gene            complement(1311..2147)
                     /locus_tag="VGC82_15530"
     CDS             complement(1311..2147)
                     /locus_tag="VGC82_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015073571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY0424730.1"
                     /translation="MRVSVMQMNPNANKPENIAQARRLIQDAVEQDRPNIVSLPEVWD
                     SLGGDRATRAANAEVLPARGSNEPGGEAYEFLRETARAAKIHVHGGSIIEQGPEKLFN
                     TTVVFNPDGTELARYRKMHLFDFVAPDGTGYRESNSYGAGDEVVTYEADGIKVGCAIC
                     YDIRFPDLFWKLREQGAEVIFLPSAFTLATGKDHWEALIRGRAIETQCWLAAPATWGK
                     HLEGKGDPRFTYGHSMIVNPWGHLVAQVSDGVGWTTARIDQDLTARIRRDMPVLEHRK
                     RV"
     gene            2516..3163
                     /locus_tag="VGC82_15535"
     CDS             2516..3163
                     /locus_tag="VGC82_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY0424731.1"
                     /translation="MKPMKVWDLPVRLFHWSIVVLIFAAWLSQELNRMEWHVRIGYTI
                     LTLLLFRLVWGVFGSDTARFLKFVRSPVAALRHLAHIRRREPDYEIGHNAAGGWMVLA
                     MLGLLGVQAGTGLFSNDDANTEGPLMHLVGKEQSDWLSHIHSLTFNFIEIFIVLHLVA
                     IGAYAMLKRQNLVRPMVTGTKLMPGDTVPPRMTGSVWAVLALALAAGAVAWLVRL"
     gene            complement(3230..3547)
                     /locus_tag="VGC82_15540"
     CDS             complement(3230..3547)
                     /locus_tag="VGC82_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424732.1"
                     /translation="MKAFPDAARLLLHAPRKEVVAALSEALGPSWAVERETDYEGEVS
                     IIALSTVDLERMPAVILYEKEGMAHVATMKCDEWENDLSFASFQQATDAFIAEAQASS
                     SHA"
BASE COUNT          725 a         1127 c         1259 g          757 t
ORIGIN      
        1 gtcggccccg gtcgctgtgg tggcggacct ggcgaactac gacgccatcg tggtcggcac
       61 cggcacccgc ttcggccgga tgtcatcgca gatggcgaac tttctcgatc aggccggggg
      121 gctttgggcc aagggcgcgc tgaacggcaa ggtgggtgcg gcattctcgt cgtccgcgac
      181 gcagcacggc ggccaggaaa cgaccttgtt ctcgattatt accaatctgc tgcattttgg
      241 catggttgtc gtcgggatgg attacggttt cgcaccgcag atgacgctgg aggaggttgt
      301 cggcggttcg ccatacggcg cgacgacgat cgccggcggc gacgggtcgc gtcagccatc
      361 acagaaggaa ctcgacggcg ctcgttacca aggccggcgg gtagccgaag tggctgccaa
      421 gctgcacggt tgagagcgaa acggtccgat ttgattcccg ccgccgccgt atcagacggt
      481 aaactgctcg gcgataatcc gttccgacag gccgcggtcc ggatcgaaca gcacagtgac
      541 ggtcacggac cgatcctcgc tgaccgccac cgacaccacg tcgcggacct cggtgaagtc
      601 cgcgaccgcg gcggtgggac gcttatccgc ctccagcacc tcgaacaata catccgcact
      661 gctgggcaac aacgcaccgc gccagcgccg cggacgaaag gcgctgattg gggtcagcgg
      721 cagcagattc gccgacagcg gcacgatcgg gccgtgcgcc gacaagttat acgcggtcga
      781 gccggcaggg gtggagatca gcacgccgtc gcaggacaac tcctccagtc gcacccgccc
      841 atcgaccgaa atccggatct tcgatgcttg gcgcaattgc cgcagcaggc tgacctcgtt
      901 gaacgccacg gcctcctcga cggacccggt ggcggtgatg acatgcatgc gtagcgggtg
      961 cagttcggct gcctgcgcgc gagacaggcg gttgggcagg tcgtcctcgc tataggtgtt
     1021 catcaggaag ccgacagagc cgcggttcat gccgtaaacc ggcacgttca agggcattac
     1081 ctggtggatg gtttccagca tgaacccgtc cccgccgagg gcaaccacaa cggatgcttg
     1141 cggcagcggg cattcgccgt agcggcggat cagattctcc cgcgcctcat gggcgctcgg
     1201 tgttggcgag gcgacaaagg cgatctggct ggccaggaac cggcgatcct cgataatcag
     1261 aggatcaggg accgggggac caggtaccgg gagatcgggc ggaagcgcac tcaaacgcgc
     1321 ttcctgtgtt ccagaaccgg catatcgcgc cgaatccttg ccgtcagatc ctggtcaatc
     1381 cgagcggtgg tccagccgac gccgtcggag acctgggcga caaggtggcc ccatgggttg
     1441 acgatcatcg aatggccata ggtaaagcgc gggtcgccct tgccctccag gtgcttgccc
     1501 catgtagccg gggctgcgag ccagcactgt gtttcgatcg cccgtccgcg gatcagcgcc
     1561 tcccagtggt cttttccggt ggccagggtg aaggcgctgg gcaggaagat cacctcggcg
     1621 ccttgttcgc gcaacttcca gaacaagtcg gggaagcgga tgtcgtagca gatcgcacag
     1681 ccgaccttga tcccgtcggc ttcgtaggtc accacctcat caccagcgcc gtagctgttg
     1741 ctttcgcggt agccggtgcc gtcgggggcg acgaagtcga acaggtgcat cttgcggtag
     1801 cgcgccagtt ccgtgccgtc cggattgaag accacagtgg tgttgaagag tttctccggc
     1861 ccctgttcga tgatcgagcc accgtggacg tggattttcg ccgcgcgggc ggtttcgcga
     1921 agaaactcgt aggcctcgcc gccgggttcg ttggaaccgc gggcgggcag cacctcggcg
     1981 ttggcggctc gggtggcgcg gtcgccgcca agggagtccc aaacctccgg cagactgacg
     2041 atgttgggtc ggtcctgctc gaccgcgtcc tggatcagcc gccgggcctg ggcaatattt
     2101 tcgggtttgt tcgcgttcgg gttcatttgc atcacgctga cgcgcatttg tgggccctcc
     2161 ggttgagcgg ccagccctgg ggcctgccga tgcgcacgga tcgccggaga aagcgtggcg
     2221 gcgaccgcgc gggatttggc gatactccgg ccgcccgccg gggcagcata tcaccagcaa
     2281 gagaagcgta tccgcgggcc ggacgcaacg gggcgggtta tgccggagca tgatcacgct
     2341 gggcggaacg tcggcggcgt gtgaatcatg cgatcaaacc attcgcatgg tcacgctggg
     2401 cggaacgtcc gaagcgtgtg aatcatgcga tcagacaagg agacagacca tttgcgccct
     2461 gcacaatcag gctggggatg gtctatggtg cggtgcatca acgtggggtg agcgtatgaa
     2521 gccaatgaag gtatgggatt tgccggtgcg gctgttccat tggtcgattg tcgtgttgat
     2581 ctttgcggcg tggctgtcac aggaactcaa ccgcatggag tggcatgtgc ggattggcta
     2641 caccatcctg acgctgctgc tgtttcgcct ggtctggggc gtcttcggca gcgatacagc
     2701 acggttcctg aagttcgtcc ggagtccggt tgccgcattg cggcatctcg cccacatccg
     2761 ccggcgggag cccgactatg aaatcggaca caacgcagcg ggcggatgga tggttctggc
     2821 gatgctaggt ttgcttgggg ttcaggccgg gaccggactg ttcagcaacg acgatgccaa
     2881 taccgaaggc cctttgatgc acctggtcgg caaggagcaa agcgattggc taagccatat
     2941 tcattctttg accttcaatt tcattgagat cttcatcgtg ttgcaccttg tggcgatcgg
     3001 tgcctacgcg atgctgaagc ggcagaacct ggtgcggccg atggtgacgg ggacgaagct
     3061 gatgccgggc gacacggtgc caccccggat gactggctcg gtttgggcag ttttggcgct
     3121 ggcgctggca gccggcgcgg tggcctggtt ggtgcggttg taggtagcca ggcggcaccg
     3181 ctgtcgccac cggcgcgccc agcgacttac tctctcctgc cgggtctgat caggcgtggc
     3241 tcgacgatgc ttgtgcctcg gcgatgaatg cgtcggtggc ttgctggaag ctggcgaagc
     3301 tcaggtcgtt ttcccactca tcgcatttca tcgtcgccac atgcgccatg ccttctttct
     3361 cgtagaggat gacagcaggc atccgttcaa gatcaaccgt ggaaagtgcg atgatcgaca
     3421 cctcgccctc gtaatcggtc tcgcgttcga ccgcccaact cggaccgaga gcttcggaca
     3481 gcgcggcgac cacctccttg cgtggcgcgt gcagcaacaa ccgggcagcg tccggaaatg
     3541 ctttcatcaa accatcgtaa gctgcatggg tcatcaaatt gcctttgaac agcgggtcgg
     3601 ggtcttacat gcggtagcgt gcaatgtttt ggctaagata aggttaaagc cacgatcgcg
     3661 atgtctaacg atttcaatta gttatcaaac cataatgaaa gcctcgcggc aagccacaaa
     3721 aaaaccgaca gaccgcaaaa aacattttct ttaacgaaaa gtttcggtag aacaccactg
     3781 gaggttgtgt taaggacgct atgcgccttc ttatggtaga aaaaatcccg tcactaagcg
     3841 attcacaaat ctgtgatgat cgaggctt
//
LOCUS       DASWQN010000911         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1934559, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000911 DASWQN010000000
VERSION     DASWQN010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934559"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /gene="mdoH"
                     /locus_tag="VGC82_15545"
     CDS             complement(<1..1346)
                     /gene="mdoH"
                     /locus_tag="VGC82_15545"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEY0424733.1"
                     /translation="MDTLTPRPGGEISGGGSRPEFLPPVSPMEMTPQALQTFSRGEDQ
                     EGAANSLGLRRACILAGTAAMSAAGCYLMYEVLQVGGVTTLEWMVLGLFVLLFTWIAF
                     SFMSALAGFMVLLFRIRDPLGIEPDAPLPSVASRNAMLLPTYNENPYRIMARLQAMYE
                     SLGQTGYGSAFDWFVLSDTTDPSVWIAEEKCFLRLREDLGVSNIFYRHRPENHARKSG
                     NIEDWIRRFGAGYEHMVILDADSLMTGDTVVRLVSAMERHPGVALIQTLPVVVNARTL
                     FSRLQQFSGRLYGPIIAAGIAWWHGSEGNYWGHNAIIRVRAFAKDAGLPELRGRKPFG
                     GHILSHDFVEAALMRRAGWAIRMVPTLGGSFEECPPSLLDFAARDRRWCQGNLQHLAV
                     LPARGLHWVSRLHLLTGIGSYLTAPLWLMFLVLGILISLQAQFVRPEYFPKGFALFP"
     gene            complement(1334..2086)
                     /locus_tag="VGC82_15550"
     CDS             complement(1334..2086)
                     /locus_tag="VGC82_15550"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HEY0424734.1"
                     /translation="MGLAPMASMVFFGPNNRVEVDDFRPSVHDSDGFAIFNGRGEELW
                     RPLNNPKDLQISTFSDLNPRGFGLMQRQKDFREYQDLESNFERRPSLWGEPIGDWGEG
                     SVQLIEIPTKEEIHDNVAGYWRPKSVLQAKGEYTYTYRLHWGPDVPKSTSLARFLRTA
                     IGARGEDAKIFVLDVVGEKLKSADAQTIRGEVTAGKAEIRNVVTQPNPATGGWRLSFE
                     LSVKDKVPIELRASLMQGNDPLSEVWVYRWTP"
BASE COUNT          450 a          654 c          616 g          401 t
ORIGIN      
        1 ggaaacaacg cgaatccctt cggaaaatat tccggcctga caaattgagc ctgcaacgag
       61 atcaagatcc ccagcacgag aaacatcagc cagagcggcg ccgtaagata ggagccgatc
      121 ccggtgagaa ggtgaagacg cgacacccaa tgcaggccgc gcgccgggag caccgcgaga
      181 tgctgaagat tgccttggca ccatcggcgg tctcgggccg cgaaatccag cagcgacggc
      241 gggcattctt caaagctgcc gccaagcgtc ggcaccatcc tgatggccca gcctgctcgc
      301 ctcatcagcg cggcctcgac gaagtcgtgg cttagaatgt gaccgccgaa cggcttgcgg
      361 ccgcgaagtt ctggcaggcc cgcatctttt gcgaatgccc gcacgcgaat aatcgcgttg
      421 tggccccaat agttgccttc ggagccgtgc caccaggcaa tgccggcggc gataatcggg
      481 ccgtatagcc gcccagagaa ttgctgcagg cgagagaaca gcgtcctggc gttgacaaca
      541 acagggagcg tctggatcag ggcgacgccg gggtgccttt ccattgcgga gacaagccgg
      601 acgacggtat ctccggtcat caggctgtcg gcgtccagga taaccatatg ttcatagccg
      661 gcgccgaatc tccggatcca gtcttctatg tttccggatt ttcgcgcgtg attttccggg
      721 cggtgacgat aaaagatatt gctgacgcca aggtcctcgc ggagtcggag gaagcatttc
      781 tcctccgcga tccagaccga agggtcggtg gtatcgctga gcacgaacca gtcgaaggcc
      841 gaaccgtatc ccgtctggcc gagcgactca tacatcgcct gaagccgggc catgatccga
      901 tacggatttt cgttgtaggt cggcaacagc atcgcgttcc ggctggctac cgatggaagc
      961 ggcgcgtcgg gttcgatgcc gagcggatcc ctgatgcgga acagaagcac catgaatccg
     1021 gccagagccg acatgaacga gaatgcgatc caggtgaaca gcagcacgaa cagacccagg
     1081 accatccatt cgagcgtcgt aacgccgccg acctgaagga cctcgtacat caggtagcag
     1141 ccggcagcgg acattgccgc agtaccggcg agaatgcagg cgcgccgtaa tcccagcgaa
     1201 ttcgcagcgc cctcttggtc ttccccacgg ctaaacgttt gcagcgcctg cggcgtcatt
     1261 tccatcggtg atacgggtgg caggaattcc gggcgcgaac cgccgcccga aatttcacca
     1321 cccggacgcg gtgtcagggt gtccatcgat agacccaaac ttccgagagg gggtcattgc
     1381 cctgcatcag ggatgcgcgc aattcaatcg gcactttgtc tttgactgaa agctcaaagc
     1441 tcagtcgcca gcccccggtc gcagggttcg gctgggtcac gacattgcga atttcggctt
     1501 tgccagcggt cacctcgccg cggatggtct gcgcgtcggc cgatttcagc ttttcgccaa
     1561 ccacgtccag aacgaatatc tttgcatcct cgcccctggc accgatagct gtccgtaaaa
     1621 accgggccag cgatgtcgac ttgggcacgt cgggccccca atgcaggcga tatgtatagg
     1681 tgtactcgcc cttggcctgc agcacgctct tcggccgcca atagcccgcg acgttatcgt
     1741 ggatttcctc tttcgttggg atctcgatca gctgaaccga gccttcgccc caatccccga
     1801 tcggctcacc ccacaggctc ggccgccttt cgaagttcga ttccagatcc tggtattccc
     1861 ggaagtcctt ctgcctttgc atcaggccga atccgcgcgg gttgagatcg gagaacgtgc
     1921 tgatctgcaa atcctttgga ttgttgagcg ggcgccaaag ctcctcgccc cgcccattga
     1981 agatcgcaaa tccgtcggaa tcgtgcacgg agggccgaaa gtcgtcaact tcaacccgat
     2041 tattcggacc gaaaaagacc atgctcgcca tcggcgcgag acccacatgg tcaagatcga
     2101 gccgaggata gatcgccatt t
//
LOCUS       DASWQN010000912         5365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1010754, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000912 DASWQN010000000
VERSION     DASWQN010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010754"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /gene="zapE"
                     /locus_tag="VGC82_15555"
     CDS             complement(<1..780)
                     /gene="zapE"
                     /locus_tag="VGC82_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEY0424735.1"
                     /translation="MNNPAPPPQQFGPLPAYQEMIANHSLAADPAQAMTVDRLQDLWE
                     RLRGYDPKPVPANGGGLLSRLMRLRHTEEYVPTGLYLVGEVGRGKSMLMDLFFDTAEV
                     ARKQRIHFHHFIQNVHARIHAYRKVHPDVSDPIPPLADQIAAESALLCFDEFQINDIA
                     DAMILGRLFQALFDRGVVVVTTSNTAPDDLFKGQPGRDAFLPFIGLIKQRLEILVMEG
                     AHDYRRERMRGLRTWLVPADEMSRRELDKAFLRLTSGSAIRP"
     gene            837..2687
                     /locus_tag="VGC82_15560"
     CDS             837..2687
                     /locus_tag="VGC82_15560"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0424736.1"
                     /translation="MTTATLPPILPAVIELVRQAGTWLAAEFARPDGPRSSDSDTSPI
                     DTEIELFLRERLTGLLPARFVGEEAGILAADGLQASANGYCWVVDPHDGTRAFLEGRR
                     GSAISVGLLRRGVPVLGVVYAPTSPDRGPDMIAWAEGDAITRNGTPVVIDLRHRDLTG
                     TDVVFLNHGAGQRPVWSSIACAPSRFTPLPSIAYRLARVAAGDGIATVTLRPVNAHDI
                     AAGHALLVAAGGVLVAEDGRPVTYGKDGDSRPSACFGGAPAAVATLRNRRWRGSTEPR
                     REAKVTLNWPRAAEDQRFDRALGCMLGMVIGDSLGSQVEFQTAASIRALYPGGVRDLA
                     ESPVWNTLAGQPTDDSELGLALARSLVRTSQYDPEDAASAYGRWYASEPFDCGLTTRS
                     AFGPAASALADKALAARSNADPGSQSNGSLMRIAPIGVWAGSPDEAAHVASLDSSLSH
                     PHPVCRTACAAFAAAISAALHGADRQGMIQTALGIADAAGPEAASVGTALRQAAAGVA
                     PADFHHQMGWVLISLQNAFYHLAAGTAVEPALVATAGSGGDTDTNAAIAGALLGAADG
                     RASLPVRWVMPVLTCRPDPGLHPARPRPDEYWPDDLLDLTEALLLSRAPS"
     gene            2684..3316
                     /locus_tag="VGC82_15565"
     CDS             2684..3316
                     /locus_tag="VGC82_15565"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY0424737.1"
                     /translation="MIELDFAKLAGTPVAADPFPHVVVPDFVPPASLDAVLADLPPLS
                     KRGSFPTDAVRLGPDAQALMQAMEGPELRAAIERLFGLDLRDSPTMVTLRGWTSDRDG
                     RIHCDSLAKRVTVLLYLNRETDAWSRREGCLRLLRGSHDLEDFAVEVPPVNGTLLVFP
                     NGPTTWHGHRPFVGQRYSVQLNYMTTDDKARSEMRRHRISAFVKRLTRAA"
     gene            complement(3392..3799)
                     /locus_tag="VGC82_15570"
     CDS             complement(3392..3799)
                     /locus_tag="VGC82_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY0424738.1"
                     /translation="MLSLIWYNVRAVPGPDKLTAFVLELLAPLGAVSARRMFGGVGLF
                     HDGMMFGLIARDELFLKVGDSNRSQYEAAGEAPFSYETKHGPHTIGSYWRCPPDLLDD
                     PEKFQAWVRKAMDAAFVAARGKPRLRHKRHATI"
     gene            3895..5352
                     /locus_tag="VGC82_15575"
     CDS             3895..5352
                     /locus_tag="VGC82_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY0424739.1"
                     /translation="MSPSDIPISVFDPDLIGRLRGRTALLTPTEMAAADKAAANHGHA
                     GPALMEAAGRAVARAIKRHIGRARVLVLAGPGNNGGDGYIAARLLAQDGWPVRVAALA
                     PPRTGSDAAGAARLWHGPTIPFTPEEAAQADVVVDAVFGAGLTRPVSGLVAETLKAAR
                     RIVAVDVPSGLDGATGLTLGYAPSAELTVTFFRLKPGHLLFPGRGLCGHVELAEIGIP
                     DSVLDAISPTTFANLPALWRLPTLTAGSHKYTRGHVTVVGGASMTGAARLAAEATRRA
                     GAGMVTIAARGAASAYRSGEPGLLVSEASIAALLQDERREVWVCGPGLGPEEARAVFP
                     ALVAANRTVVGDADVFSAFSGQPDALRGAAVLTPHAGEFARVFGSPGTDRLSAAREAA
                     ARTGAVLLLKGADTIIAAPDGRAAINLSAPPWLATAGAGDVLSGIIAGLLAQGMPAWD
                     AAAAGAYLHGRAATLVGPGLIVEDLLPALIRAIAN"
BASE COUNT          860 a         1767 c         1792 g          946 t
ORIGIN      
        1 gggcctgatc gccgaaccgc tggtcaggcg caggaacgcc ttgtccagtt ccctgcgaga
       61 catttcatcc gccggcacca gccatgtgcg caggccgcgc atccgctccc gccggtagtc
      121 gtgcgcgcct tccatcacca ggatttcgag ccgctgcttg atcaatccga taaacggcag
      181 aaacgcgtcc cggccgggtt ggcccttgaa caggtcgtcc ggcgcggtat tggaggtcgt
      241 tacaacgacg acgccacgat cgaacagcgc ctggaacagc cgcccgagta tcatcgcatc
      301 ggcgatgtcg ttgatctgga actcgtcgaa gcacaacagc gccgattcgg cggcaatttg
      361 gtcggcaagc ggaggtatcg ggtcggacac gtccggatgg accttcctgt aggcatggat
      421 gcgggcgtga acgttctgga tgaaatgatg aaaatggata cgctgcttgc gcgccacctc
      481 cgcggtatcg aaaaacaggt ccatcagcat cgacttcccg cgcccgacct cgcccaccag
      541 ataaaggccg gtcggcacgt attcctcggt gtgccgcaga cgcatcagcc gcgacagcag
      601 tccgccgcca ttcgccggaa ccggtttggg atcgtagcca cgcaatcgct cccatagatc
      661 ctgcaggcga tcgacggtca tcgcctgcgc cggatcggcc gccaacgagt ggttggcgat
      721 catctcctgg taggccggca gcgggccgaa ctgctgcggc ggtggggcgg gattgttcat
      781 ggtgggttcg gttgagatag cgtgtcccgg cacgaacgcc tagacttctc cgccccatga
      841 cgacagcaac cctgcccccc attctccccg ccgtgatcga gcttgtaaga caggccggca
      901 cttggctcgc cgccgaattc gcccggcctg acgggccaag atccagcgac agtgacactt
      961 cgccgatcga taccgagatc gagttgttcc tgcgcgaacg cttgaccggc ttgctgccgg
     1021 cccgcttcgt gggcgaggaa gccggcatac tggcagccga tgggctacaa gcctcggcta
     1081 acggatactg ctgggtcgtc gatccgcatg acggcacgag ggcattcctg gagggccgcc
     1141 ggggcagtgc gatctcggtc gggttgttgc gccgcggcgt cccggttctt ggcgtggtct
     1201 atgccccgac cagccccgac cgaggccccg acatgatcgc gtgggccgag ggcgatgcca
     1261 tcacccgcaa cggcacgccg gtggtaatcg acctaaggca ccgggatctc accgggactg
     1321 acgtggtatt cctgaaccat ggcgctgggc agcggccggt ctggagcagc atcgcctgcg
     1381 cgccctcacg tttcacgccg ctgccctcca tcgcctaccg gctggctcgg gtcgcggccg
     1441 gcgacggcat tgccaccgtt accctgcggc cagtcaacgc acacgacatc gccgctgggc
     1501 atgcgctgct ggtcgccgcc ggcggcgtgc tcgttgccga ggacggtcgg ccggtcacct
     1561 atggcaaaga cggcgatagc cggccatccg cctgcttcgg tggtgcaccg gccgcggtgg
     1621 ccacgttgcg aaaccgaaga tggcgtggca gcaccgaacc gcggcgagag gcgaaggtga
     1681 cgctgaactg gcctcgggcg gcggaagatc agcgcttcga ccgagcattg ggctgcatgt
     1741 tgggaatggt cattggcgac agcctggggt cccaggttga atttcaaacg gccgcgtcga
     1801 tccgggcctt gtaccccggc ggggtgcgcg atctggccga gagcccggtg tggaacacgt
     1861 tggcgggaca gccgacagat gattcggaac ttggcctggc gctggctcgc agcctggtgc
     1921 ggaccagcca gtacgaccca gaggatgccg cttcggcgta cgggcgctgg tatgccagcg
     1981 aaccattcga ctgcggcctc accacccgat cggcgtttgg tcccgcagct tcggctttgg
     2041 cggacaaggc cctggccgcc cgctcgaacg ccgatccggg cagccaaagc aacgggtcgt
     2101 tgatgcgcat cgcgccgatc ggtgtttggg ccggcagtcc ggacgaagcc gcgcatgtgg
     2161 ccagtttgga ctcttcgctg tcccacccgc acccggtgtg ccgcaccgca tgcgcggcct
     2221 tcgccgcagc catttccgcc gcgctgcacg gtgccgatcg gcaaggaatg atccaaaccg
     2281 cgctggggat tgccgatgcc gccggcccgg aggctgcttc ggtgggtacg gctttgcgcc
     2341 aggctgcggc gggcgttgcc ccggcggatt ttcatcacca gatgggatgg gtgctgattt
     2401 ctttgcaaaa tgctttctat cacctcgcgg cgggcacggc ggtggaaccc gccttggtcg
     2461 ccacggccgg aagcggcggc gacaccgaca ccaatgccgc catcgccggc gccctgctcg
     2521 gtgcggcgga tggccgcgca tcgctgccgg tgcggtgggt gatgccggtg ctgacgtgcc
     2581 ggcccgatcc cgggctgcat ccggcgcgac cacgacccga cgaatactgg cccgacgacc
     2641 tcctcgacct gaccgaggct ttgctgctaa gccgcgcgcc gtcatgattg aactcgattt
     2701 cgccaagctg gccggcacac cggtcgcggc cgaccctttc ccgcatgtgg tggtcccgga
     2761 cttcgtgccg ccggccagcc tggatgcggt gctggcggat ctgccgccgc ttagcaaacg
     2821 tggctctttt cccaccgacg cggtcaggct cggtcctgac gcacaggcgc tgatgcaggc
     2881 catggaaggt cccgaattgc gcgccgcgat cgagcggttg ttcggcttgg acctgcgcga
     2941 ctcgccgacc atggtgacgt tgcgcggctg gaccagcgac cgggacgggc ggatccactg
     3001 cgactcgctg gccaagcggg tgacggtgtt gctgtatctg aatcgcgaga ccgacgcatg
     3061 gagccggcgg gaaggttgcc tgcgcctgct gcggggatcc catgacctgg aggatttcgc
     3121 cgtcgaggtc cccccggtca atggcacgct gctggtattt ccgaatggtc cgaccacgtg
     3181 gcacggccat cgtccgttcg tcggtcagcg ctacagcgtg caactcaatt acatgacaac
     3241 ggacgacaag gcccgcagcg agatgcgccg ccaccggatt tccgcgttcg tgaagcgttt
     3301 gacacgcgcg gcttgaggcg atggttgggg tatttcggga ctgtgcgacc cgcggaatgt
     3361 ggctgtttcg cttcatgcgc gaaggacggg cttaaatggt ggcatgtcgc ttgtggcgca
     3421 gtcttggttt gccgcgagcc gcgacgaaag ccgcgtccat agctttgcgc acccaggcct
     3481 gaaatttctc cggatcgtcc agcaggtcgg gcggacagcg ccaataggaa ccgatcgtat
     3541 gtggtccgtg ctttgtctca tagctgaacg gagcctctcc ggccgcctcg tactgtgagc
     3601 gattgctgtc gcctaccttg aggaacaact cgtcgcgggc gatcaggcca aacatcatac
     3661 cgtcgtggaa aagtccgacg ccgccgaaca tccgccgcgc ggaaacagcg cccagtggcg
     3721 cgagcagttc cagcacaaag gcggtgagtt tgtcggggcc gggcacggca cgcacgttat
     3781 accagatcag cgacagcaaa agagcggtcg cgggtcagta gcagccgaag catgaggcgt
     3841 tgccgcagat cccgaacggg gcttgcataa aggctactcc acaggatctt gacgatgtcc
     3901 ccatccgata tccccatctc ggtcttcgac cccgatctga tcggccgcct gcgtggccga
     3961 accgcgttgc ttacacccac tgaaatggcg gcggctgaca aggccgcggc caaccacgga
     4021 cacgccggtc ctgccctgat ggaggccgcc ggccgcgccg tggcccgggc catcaagcgc
     4081 cacattggcc gcgctcgtgt gctggttctg gcgggtcccg gcaacaatgg cggcgacggc
     4141 tacatcgccg cgcgtcttct ggcgcaggac ggttggccgg tgcgcgttgc cgctctggcc
     4201 ccgccccgca ccgggtccga cgccgccggc gccgcgcgct tgtggcacgg cccgacaata
     4261 cctttcacgc cggaagaagc cgcccaggcg gacgtggtgg tcgatgcggt attcggcgcc
     4321 ggtctgacgc ggccagtctc cggcctggtc gcggagacac tgaaagccgc gcgccggatc
     4381 gttgcggtcg acgttccctc cggcctcgac ggggccaccg gactgaccct tggttacgct
     4441 ccgtcggccg aactgaccgt tactttcttt cgcctcaagc ccggccactt gctgtttccg
     4501 ggccgcggcc tctgcggcca tgtcgaactg gccgagatcg gcatccccga ttccgttctg
     4561 gacgctatct cgcccaccac cttcgccaac ctgccggcct tgtggcggct gccgacgctg
     4621 accgccggat cgcataaata tacccgcggc catgtgaccg tggttggcgg cgccagcatg
     4681 accggagccg ctcggttggc cgcggaagcc acccggcgag cgggtgccgg tatggtaacg
     4741 attgccgcgc gcggggctgc cagcgcctat cgatccggcg agccaggcct gttggtcagc
     4801 gaggccagca tcgccgcact cctgcaggac gaacgccggg aagtctgggt gtgcggtccc
     4861 ggccttggtc cagaggaagc gcgcgccgtc ttccctgccc tggtcgcagc gaaccggacg
     4921 gtggtcggcg atgcggatgt gttctcggca ttcagtggcc agcctgacgc gctgcgaggc
     4981 gcggccgtgc tgacgccgca tgcgggcgag ttcgccaggg ttttcggcag tcccggcacg
     5041 gacaggttat cggccgcgcg agaagccgcg gcgcgaaccg gtgccgtatt gctgctaaaa
     5101 ggagccgaca cgatcatcgc tgctccggac ggccgggccg caatcaactt gtccgcgccg
     5161 ccgtggctgg cgacggccgg cgcgggggat gtgctttccg ggattattgc cggtcttttg
     5221 gcccagggta tgccggcatg ggatgccgca gccgcgggcg cttacctgca tgggcgagcg
     5281 gcgacgctgg ttggtcccgg actcatcgtc gaagatctgc tgccagcact tattcgtgcg
     5341 atcgcaaatt aaaactgaga cgcca
//
LOCUS       DASWQN010000913         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2198821, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000913 DASWQN010000000
VERSION     DASWQN010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198821"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..501
                     /locus_tag="VGC82_15580"
     CDS             55..501
                     /locus_tag="VGC82_15580"
                     /inference="COORDINATES: protein motif:HMM:NF007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEY0424740.1"
                     /translation="MHRLTQQGTGEELGRIVISGSAGGAVFKLDLHGLPPGPHGFHVH
                     ENSGCGPTNMNDVRIPGGAAGGHLDPDHANKHEGPTGEGHRGDLPVLTVEANGTATQK
                     LTAPRIKDIESLRNHALIIHAGGDTYSDTPNALGGGGGRLACGVIE"
     gene            507..1574
                     /locus_tag="VGC82_15585"
     CDS             507..1574
                     /locus_tag="VGC82_15585"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0424741.1"
                     /translation="MEFGIALATAADSWKTVKRAEELGFSHAWFYDTQMLCADCFVAM
                     GAAAALTSRIRLGTGVLIPTNRIAPVAANAFASLNQLAPGRIDFGVGTGFTGRRTMGL
                     GAMKQSDMEEYIRVVMALLDGETPEFEIEGKRQKITFLNPELKLINLRDEVKLHVSAY
                     GPRGRALTARLGAGWLNFVGDVTTGVAAMREMQQSWDRGGNDIVDLTAVAFALGCILQ
                     PGEAADSDRAMAQAGPRAAVLLHRAADEALTGLPNSSPVLPELAEPIAGYVELAKTFK
                     PADAPYLTNHRGHLMAVKSIERPFVTAEMIRRTTFTGTEPDLRERIAALRDGGYSQFT
                     IQLVPGQEAAIEDWARLKAAF"
     gene            complement(1597..2472)
                     /locus_tag="VGC82_15590"
     CDS             complement(1597..2472)
                     /locus_tag="VGC82_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEY0424742.1"
                     /translation="MHRRSFTAALTLVPFAALAVEKPRVVATFSILANLVSQVSGGAV
                     SVEALVGPDGDVHVYEPRPKDLRTLLAAALVVCNGLGLEGWMDRLTAASGYKGRIVVA
                     AEKVTPRTMTENGGAIATDPHAWQDPRNAILYVQAIADGLASADPSGAATYHDAASRY
                     VAEIAQTDTWIETALAAIPPERRRIITTHDAFGYYGARFGIELLSAEGISTESEPSAK
                     AIAALVRQIEREKVRAVFIENMTSPRIAQMLARETGAVLGGIVYSDALSPPGGPAPSY
                     LDMLRHNTTQFAKAM"
BASE COUNT          459 a          838 c          807 g          451 t
ORIGIN      
        1 gttatggcgc ttgccgccgc tgttcccgcg gcagcagcgg atccaacggt cgccatgcac
       61 aggctgacgc agcagggaac cggcgaagaa cttggcagga ttgtcatctc cggttcggct
      121 ggtggtgccg ttttcaagct cgatctgcac ggcttgcccc ctggcccgca cggcttccat
      181 gtgcacgaaa acagcggttg cggcccgacc aatatgaatg acgtccgcat tccgggcggc
      241 gcggccggtg gccatcttga cccggatcat gccaacaagc acgagggtcc gacgggcgag
      301 ggccatcgcg gtgaccttcc cgtgctgacc gtcgaggcca acggcaccgc gacgcaaaaa
      361 ctgaccgcac cgcgcatcaa ggacatcgaa tcgctcagga accacgcgct gatcatccat
      421 gccggcggcg acacctacag cgacaccccc aatgcgctcg gcggcggcgg tggacgcctc
      481 gcctgcggcg tgatcgagta gccgccatgg aattcggtat cgccctcgcc accgcggcgg
      541 atagctggaa aacggtcaag cgcgccgagg aactgggttt ctcgcacgcc tggttttatg
      601 acacgcagat gctctgtgcg gactgctttg tcgccatggg cgcggccgcg gccctgacct
      661 cccgcatccg gctcggcacg ggcgtgctga tcccgaccaa ccggatcgcg cccgtcgccg
      721 ccaacgcatt cgcctcgctg aaccagctcg cacccggacg gatcgatttc ggcgttggca
      781 ccgggttcac cggacggcgc acgatgggcc ttggggcgat gaaacagtcc gacatggagg
      841 aatacatccg cgtcgtcatg gccttgctcg acggtgaaac ccccgagttc gagatcgagg
      901 gcaagcgcca gaagatcacc ttcctcaatc ccgagcttaa actgatcaac ctgcgtgacg
      961 aggtaaagct gcatgtttcc gcctatggcc cgcggggccg agcactgact gccaggttgg
     1021 gggcgggttg gctgaacttc gtcggcgacg tgacgaccgg agtcgccgcg atgcgggaaa
     1081 tgcaacaaag ctgggaccgg ggcggcaacg acatcgtcga tcttacggct gtcgcatttg
     1141 ccctgggctg catcctgcaa ccaggcgagg cagccgacag cgaccgggcg atggcacagg
     1201 ctggcccgcg ggcggctgtg ctgctgcacc gcgcggccga tgaggcgttg accgggctgc
     1261 caaacagctc gccggtactg cccgaactgg cggagccgat cgctggctat gtggaattgg
     1321 cgaagacgtt caagccggcg gatgccccct acctgaccaa tcatcggggt cacttgatgg
     1381 cggtaaagtc catcgagcgg ccgttcgtga ccgccgaaat gatccggcga accaccttta
     1441 ccggcactga acccgatctg cgggagcgga ttgctgcctt gcgcgacggc ggctactcgc
     1501 agttcaccat ccagctcgtc cctggccagg aagccgccat cgaagattgg gcaaggctga
     1561 aagcggcttt ctaaaccaag gtcacgggct tcgaagctac atcgccttgg cgaactgcgt
     1621 cgtgttgtgc cggagcatgt ccaggtagct cggtgcgggt ccgccgggcg gggacagggc
     1681 gtccgaatag actatcccgc ccagcaccgc gccggtttcc cgcgccagca tttgcgcgat
     1741 ccggggactg gtcatgttct cgatgaacac tgcccgcacc ttctcgcgct cgatctgccg
     1801 caccagcgcg gcgatggcct tggcggacgg ttcggactcc gtgctgatac cctccgccga
     1861 caaaagctcg atcccgaagc gcgcgccgta atagccgaac gcgtcatgcg tcgtgatgat
     1921 ccgccttcgt tcgggcggaa tggctgctag tgccgtctcg atccaagtgt cggtctgcgc
     1981 gatttcggcg acgtagcgag atgccgcatc gtggtacgtt gccgcacccg atgggtccgc
     2041 tgatgccaga ccatcggcga tagcctggac atacaagatc gcgttccgtg ggtcctgcca
     2101 ggcgtgcggg tcggtggcga tggcgccgcc gttctccgtc atggttctgg gcgtcacttt
     2161 ttccgccgcg accacgatcc tgcccttata gccggacgcg gcggtcaacc ggtccatcca
     2221 gccctccagc cccagcccgt tgcataccac cagtgccgcg gccagtagcg tccgcaggtc
     2281 ctttggacgg ggctcatata catgaacatc gccgtccggc cctaccaatg cctctaccga
     2341 gacagcgccg ccgctaactt ggctcaccag gttggccagg atggaaaacg tggccaccac
     2401 gcgtggcttc tccaccgcca aggcggcgaa cggcaccaga gtcagggccg cggtaaatga
     2461 tcgccggtgc atcgtgcgga tccgttatgt tatactataa cattagaggt agatgctctg
     2521 gcgttcaagc ggtcagcccc agcggtcagc cccag
//
LOCUS       DASWQN010000914         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2462070, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000914 DASWQN010000000
VERSION     DASWQN010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2462070"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..682
                     /locus_tag="VGC82_15595"
     CDS             155..682
                     /locus_tag="VGC82_15595"
                     /inference="COORDINATES: protein motif:HMM:NF037150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY0424743.1"
                     /translation="MLAAARRLFAAEGYERTTVRAIAAAAEADPALVVRYFGGKEGLF
                     ALAAAFDLRLPDMSSVPREARGAALVRHFLQRWEGDPDDHALRILLRTAVTNPEAAGR
                     MRDIFSWQLLPAMARAAPDGEGERRAGLVASQVLGFAFCRYVLELPAVVAMDREAVVA
                     SLGPTLQRYLTEACL"
     gene            complement(842..1924)
                     /locus_tag="VGC82_15600"
     CDS             complement(842..1924)
                     /locus_tag="VGC82_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY0424744.1"
                     /translation="MRGRDHQRGQLGGYVSPEAMVPADHPLRTIRPLVNTALERLSPD
                     FDEIYSWTGRPSIPPEQLLRALLLQAFFTVRSERPLIEQLSYNILFRWFVGLSIEAPV
                     WDVTVFTKNRDRLLEGDIAHGFLRAILADPQVKRLLLNEHFSVDGTLIEAWASMKSFR
                     PKDRSGEPPGRGRNAERDFHGETRSNETHASTTDPDARLYKKAKGQAAKLCHMGHVVI
                     ENRSCLVIDTRTTHATGTAEREAAVAMIAAIPGRHRITVGCDKAYDTTDFVADIRGLE
                     ATPHVTQNDNGRRSAVDGRTTRHWGDQVSLLVRKRIEEAFGWIKTIGGQRKTRYRGTP
                     RVGWMFTLSMTAYDLIRLPKLLGAAA"
     gene            2083..>2232
                     /locus_tag="VGC82_15605"
     CDS             2083..>2232
                     /locus_tag="VGC82_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEY0424745.1"
                     /translation="MDLASDSGGSEGRFNTYLDALTATLGHADRAAPFQSYCTGLLLP
                     GERKSV"
BASE COUNT          375 a          706 c          708 g          443 t
ORIGIN      
        1 agggctcgtc agagaataac cgctcgatct cactggttgg ccacgacgac ggttccgcaa
       61 gttatccaag gggccacggc ccaaatcgta aaacgaagtt tcgggtggag ctcaaagcca
      121 gttcggttat tccctgacgc accctaaggc tcgcattctc gccgcggcgc ggcgcttgtt
      181 tgccgccgaa ggctacgagc gcaccacggt ccgcgctatc gccgcggccg cagaggcgga
      241 cccggccctg gtcgtgcgtt actttggcgg caaagagggg ctgtttgcat tggccgccgc
      301 cttcgacctg cgtctgcccg acatgtcgag cgtcccgcgc gaggcacggg gtgcggcgct
      361 ggtccggcac ttcctgcagc gctgggaagg cgaccccgac gatcacgcct tgcgcattct
      421 gctgcgcacg gcggtgacga accccgaagc cgctgggcgt atgcgggaca tcttctcatg
      481 gcagttgctg cccgcgatgg cgcgggcggc gccggacggg gagggtgagc gccgggccgg
      541 cttggtcgcg agccaggtgc tgggctttgc tttctgccgc tatgtcttgg agttgccggc
      601 cgtggtggcc atggaccgcg aggccgtggt cgcctcactc gggccgaccc tccagcgcta
      661 cctgactgag gcctgccttt agcaggctgc ggaaataccc ctgaacgatt gctatttcct
      721 gagtttttgg ctgccggggc aggctgaccg tggatagtga gaggcaattg tgagagattc
      781 tgccgtgctg gccgatgaaa cttacattca cagccaggca acgaacagac tccaggtgcc
      841 gctacgccgc ggctcccagc agcttcggca ggcggatcag gtcataggcg gtcatcgata
      901 gcgtgaacat ccagccgacg cgcggcgtgc cgcgatagcg cgtcttgcgc tgtccaccga
      961 tcgtcttgat ccagccgaac gcctcctcga tccgtttgcg cacaagcagg ctcacttggt
     1021 cgccccagtg gcgtgtggtg cgcccgtcaa cggcggagcg gcggccgttg tcattctgcg
     1081 tcacatgcgg ggtcgcctca aggccgcgga tatcggcaac gaagtcggtg gtgtcgtagg
     1141 ccttgtcgca gcccacggtg atgcgatgcc gccccggtat cgctgcaatc atcgctaccg
     1201 ccgcctcgcg ctcggcggtg ccggtcgcat gcgtggttct ggtgtcgatc accaggcagg
     1261 aacggttctc gatcaccaca tggcccatgt ggcacagctt cgccgcctgg cccttggctt
     1321 tcttgtaaag ccgggcatcg ggatcggtcg tcgaggcgtg cgtctcgttg ctgcgtgtct
     1381 cgccatggaa gtcgcgctcg gcgttgcggc cacgaccagg tggctcaccg ctgcggtcct
     1441 tcggccggaa actcttcatc gaagcccaag cttcgatcag ggtcccgtcg accgagaagt
     1501 gctcgttcaa gagcagccgc ttcacctgcg ggtcagccag gatcgcgcgg aggaagccat
     1561 gggcgatatc gccctccagc aaccggtcgc ggttcttggt gaacaccgtc acgtcccaca
     1621 ccggcgcctc gatcgacagg ccgacaaacc agcgaaacag gatgttgtag ctcaattgct
     1681 cgatcaacgg ccgctccgag cggaccgtga agaacgcctg cagcagcagg gcccgcagca
     1741 gttgctcggg tgggatcgag ggccggccgg tccacgagta gatctcatcg aagtcaggtg
     1801 aaagacgctc cagggctgtg ttcaccaacg ggcggatcgt ccgtagggga tgatccgccg
     1861 gcaccatcgc ctccgggctc acgtagccgc caagctgacc tctctgatga tccctcccgc
     1921 gcatccgtcg gtctccgtgc ccgttccagt cttaggaaat caaatcgatg gcggatttta
     1981 caagaggttt ttccgcagca tgttagtctt actgtgtcat aagagcttga tatcttaacg
     2041 gcaacttccc aactttcggc ctctcaacta tggaggcggt ttatggatct tgcgtccgat
     2101 agtggtggca gtgaaggccg tttcaacacc tatctcgatg ctctgactgc cacgctcggc
     2161 cacgccgatc gtgccgcgcc gttccagtcc tattgtaccg ggctgctgct accgggcgaa
     2221 cgcaagagcg tc
//
LOCUS       DASWQN010000915         4279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1010936, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000915 DASWQN010000000
VERSION     DASWQN010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010936"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VGC82_15610"
     CDS             <1..1160
                     /locus_tag="VGC82_15610"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0424746.1"
                     /translation="VHLSLPSDVLDATIPESTITWPAEADFTPRPLPLGDAAADAVLA
                     LLATAKRPLIVGAPVLATAKGRDLLRRIEKALSIPACLSEGPRGFNDASLGTYADVAK
                     RTDMVLLLGKALDFTLKFGGAPFPPNCRFIAIDPDGTILDRAARSRGLAFGCVADTYP
                     AAEALIARGSALGDPAWLEDARSCFDDRPPEWADLASRTHGKLHPAQVFRTLQPFLDR
                     DNNSVLICDGGEFAQWGQSMLRNDRRMINGVAGSIGSSLPMAAGARVVEKAAPVFAVL
                     GDGTFGFHMAEIETAVRLDLPYVAIVGTDCRWNAEYNLQVRDYGPNRTHGCELNPTRY
                     DLVAAALGGHGELVTSVDELAGAIERGLASGKPAVINVMIESIPSPIIRRS"
     gene            1164..2177
                     /locus_tag="VGC82_15615"
     CDS             1164..2177
                     /locus_tag="VGC82_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY0424747.1"
                     /translation="MRLYRLPEASGIDSLTMAKGDVPAPGRGQVLVRMHAASLNYRDL
                     MIVNGRAARGAPPANLVPLSDGAGEVESIGPEVTRVKPGDRVAACFMQSWIGGPIGSD
                     DAASSLGGAIDGVLSEYRLFDQIGLVHLPEHLSFEQGATLPCAGVTAWNALFGGQTLY
                     PGDSVLVLGTGGVSVFALQLAVAAGLTVVATSSSDEKLARMQRLGATHLVNYRQHPEW
                     QDQVVAATGGGVARVVEVGGPGTLARSIMATRQGGEIGMIGALTTGGEINPTTAMRKG
                     LIMRGIYVGSRQMFEAMNRAIAAHRIEPVIDRVFQFEQAADAYRHQTSQTHLGKVVIR
                     ISA"
     gene            2287..3084
                     /locus_tag="VGC82_15620"
     CDS             2287..3084
                     /locus_tag="VGC82_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0424748.1"
                     /translation="MLTEDQVRQFHEEGYLFLPETFTLTEVAILNSEADGIYRQQRPE
                     VWREKSGAPRTAFAAHLYNEAHGLLARHPSMIRPVEQLFGEQVYMHQYKINAKASFTG
                     DVWQWHQDYGTWARDDGMPEPRAMNIAVFLDEVMPINGPLMLVPRSHTQGVLAAEHDT
                     ATTSYPLWTLDEDTVTQLVAKGGIVAPTGKPGGLLLFHGNLVHGSAGNITPYPRKIVY
                     LTLNAVSNYIRTPTRAEWIAHTDFTPIAPTDPEALTRYAQRFRQAAE"
     gene            3166..>4279
                     /locus_tag="VGC82_15625"
     CDS             3166..>4279
                     /locus_tag="VGC82_15625"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0424749.1"
                     /translation="MRRRKMILGAGAGLLASPAVAAEATRLAADLPAGTRDVARFVNL
                     PGKQRMLRLTDRPPNYATPVNVFTEAVTPNDRFFVRYHLAGVPSAADMDGWSLSITGN
                     AVDRPVQLKMSDLLDLPTNQVLAVNQCAGNRRGLTVPHVAGVQWDSGAMGCAVWRGPA
                     LREILKAAGVKAEALEVRFAGADKPPMDGTPAFEKSLPLEKAMDLDTILAISMNNAPL
                     PLLNGYPARLVVPGWTGTYWMKHLTRIEVSSEPLTNFWMKAAYRVPAGMFPVERPFRS
                     QATETTVPITELVVNSLIADPLDGAGLERSGTTIRGVAWDRGAGISRVEVSLDGGRNW
                     RDALLDRPLGRYAYRRFSLETGPMPAGTYQFASRATS"
BASE COUNT          764 a         1354 c         1406 g          755 t
ORIGIN      
        1 cggtgcacct gagcctgccg tccgacgtgc ttgacgcaac catacccgaa agcaccataa
       61 cctggccggc cgaagccgat ttcaccccaa gaccgctccc tctcggcgat gccgcggcgg
      121 atgcggttct tgccctgctg gccacggcca aacgccctct gatcgtgggc gcgccggtgc
      181 tggcgaccgc caagggccgc gacctgctgc gccggatcga gaaggctttg tcgatccccg
      241 cctgtctcag cgaaggccca agaggcttca acgatgccag cctgggcacc tacgccgatg
      301 tcgccaaacg cacggacatg gtgctgctgc tgggcaaagc actggatttc accctgaagt
      361 tcggcggcgc gccgttcccg ccgaattgcc gcttcatcgc aatcgaccca gatggcacca
      421 ttctggaccg agcggcgagg tctcgagggc tggcattcgg ctgcgtggcc gatacctacc
      481 cggcggcgga agcgttgatc gcccgcggtt cggctctcgg cgatcctgcc tggctggaag
      541 atgcgcgttc ctgtttcgac gaccggcctc cggagtgggc cgatctggca tctcggacgc
      601 acggcaagct gcatccggcg caggtgtttc gaacgctgca gccattcctc gatcgcgaca
      661 acaacagcgt gctgatctgc gatggcggcg agttcgcgca gtgggggcaa agcatgctgc
      721 gcaacgaccg gcgcatgatc aacggcgtcg ccggctccat cggctcgtcg ctcccgatgg
      781 ccgcaggtgc tcgggttgtc gagaaagccg ccccggtctt cgcggtattg ggcgacggca
      841 cgtttgggtt ccacatggcg gagatcgaga ccgccgtgcg gttggacctg ccctatgtcg
      901 cgatcgtcgg cacggattgc cgatggaacg ccgagtataa cctgcaggtg cgggactacg
      961 gcccgaaccg cacgcatggc tgcgagctga acccgacccg ctacgatctt gtggcggcgg
     1021 cgcttggcgg acatggcgaa ctggttacca gcgtggatga actcgccggc gctatcgagc
     1081 gcggtctggc cagcggcaag ccggcggtga tcaatgtcat gatcgagagt atcccctctc
     1141 ccatcatacg gcggagctga acgatgcggc tgtatcgact tcctgaggcg tcgggcatcg
     1201 actcgctgac catggcaaag ggcgatgttc cggcaccggg tcgcggtcag gtgctggttc
     1261 ggatgcacgc cgcatcgctg aactaccgcg acctgatgat cgtgaacgga cgagccgcac
     1321 gcggtgcgcc accggccaac ctggtgcctc tgtcggatgg cgctggcgaa gtcgagtcga
     1381 tcgggccgga ggtgacgcgg gtcaagccgg gcgaccgggt tgccgcatgc ttcatgcaaa
     1441 gctggatcgg tgggccgatc ggttcggacg atgcggccag ttccctcggc ggcgcgatcg
     1501 acggcgttct gtcggagtac cggctgttcg accaaatcgg actggtgcat ctgcccgaac
     1561 atttatcgtt cgagcagggc gcaaccttgc cttgtgccgg ggtgacggcc tggaacgcgc
     1621 tgttcggcgg ccaaacactg taccccggcg acagcgtgct ggtgctgggc accgggggcg
     1681 tgtcggtgtt cgccttgcaa ttggcggtgg cggccgggct gacggtggtt gcgacctcgt
     1741 cgtccgacga gaagctggcg aggatgcagc gcctgggcgc gacacatctg gtgaactatc
     1801 gccagcatcc ggaatggcag gatcaggttg tcgcggcgac gggtggtggc gtggcgcggg
     1861 tcgtggaagt cggcggccct ggtaccctgg cgcgatccat catggcaact cgccagggcg
     1921 gcgagatcgg gatgatcggc gcgctgacga cgggcggaga gatcaatccg acgaccgcga
     1981 tgcgcaaggg cctgatcatg cgggggatct atgtcgggtc ccgccagatg ttcgaagcga
     2041 tgaaccgagc catcgccgcc caccgcatcg aaccagtgat cgatcgggtt ttccaattcg
     2101 agcaggccgc tgacgcctat cggcatcaga ccagccagac gcatcttggg aaggtggtga
     2161 tccgaatttc ggcataactc cgatgggcgg acccccgcat aatcgcgggt gaagccgcgg
     2221 ccatcgcggc acgcccgact gtcgaagaac tgcaaccaaa ttcgcttgaa gcacaggaga
     2281 cagaacatgc tgaccgaaga ccaagtccgc cagttccatg aggaaggcta cctcttcctg
     2341 ccggaaacat tcaccctgac cgaagtggcg attctcaatt cggaggctga cgggatttac
     2401 cgccagcagc gcccagaagt ctggcgggag aagtctggcg caccccgcac cgcctttgcc
     2461 gcgcatctat ataacgaggc acacggcttg ctggcgcgcc atccaagcat gatccggccg
     2521 gtcgagcagt tgttcggcga gcaggtttac atgcaccagt acaagatcaa cgccaaagcg
     2581 tctttcaccg gcgacgtgtg gcaatggcac caggactacg gcacctgggc gcgcgatgat
     2641 ggcatgcccg aacccagggc catgaacatc gcggtatttc tcgatgaggt gatgccgatc
     2701 aacggcccgc tcatgctggt gccgcgcagt cacactcagg gcgtgctggc cgccgaacac
     2761 gacaccgcca cgacgtcgta cccgctttgg acgctcgatg aggacactgt cacccagctg
     2821 gtagccaaag gcggcatcgt cgcaccgacc ggcaagcccg gcggcctgtt gctgttccac
     2881 ggcaacctcg tgcatgggtc ggccgggaac atcacgccgt atccgcgtaa gatcgtctat
     2941 ctgacgctga acgccgtctc caactatatt cgcacgccaa cccgggcaga gtggatcgcg
     3001 cacacggatt tcaccccgat cgctcccacc gatcccgagg cgctgacccg ctatgcgcaa
     3061 cgtttccgtc aagccgccga gtaggtgcgg attgcaccgt gcccgattta aggcgaactt
     3121 ttcctgatcg gccggtaacg tgccgggatg cgggcgggga aagacatgcg gcggcgaaaa
     3181 atgatcctgg gggcaggggc gggattgctg gcctcacctg cggtggcggc tgaggcaacc
     3241 cgtcttgcgg cggatctgcc cgcgggtacg cgggacgttg cgcgcttcgt caatctgccg
     3301 ggcaagcagc ggatgttgcg cctgaccgat cggccgccaa actacgcaac gccggtgaac
     3361 gtatttacgg aagctgttac ccccaatgac cgcttctttg tccgctacca cttggcaggg
     3421 gtgccgtccg cggccgacat ggacggctgg tcgctgagca ttaccggcaa tgccgtcgat
     3481 cgcccggtcc aactgaaaat gagcgatctg ctggatctgc caacgaacca ggttctggcg
     3541 gtcaaccagt gtgccggcaa tcgtcgcggc ctgacggttc cccatgtcgc gggcgtgcag
     3601 tgggacagcg gcgccatggg atgtgcggtt tggcgcggtc cggcccttcg cgagatcctg
     3661 aaagccgcag gcgtcaaggc ggaggcgctg gaggttcgct ttgccggcgc ggataaaccc
     3721 ccgatggacg gtacgccggc gttcgagaag agcttgcccc tcgaaaaagc catggacctc
     3781 gataccattc tggccatctc catgaacaat gcgccgctgc cgttgctcaa cggctatccg
     3841 gcgcggcttg tcgtgcctgg ctggacgggc acgtactgga tgaagcacct gactcgcatc
     3901 gaggtttcga gcgaaccgct gacgaatttc tggatgaagg ccgcctatcg cgtgcccgcg
     3961 ggcatgtttc cggtcgaacg cccgttccgc tcgcaggcca ccgaaacgac agttccgatc
     4021 accgaactgg ttgtcaactc gctgatcgcc gatccgctgg acggtgctgg gctagagcga
     4081 agcggcacca cgattcgcgg tgtcgcctgg gatcgcggcg ccggcatcag ccgcgtcgag
     4141 gtgtcgctcg acggcggccg caactggcgt gacgcgcttc tggaccggcc gcttggacgc
     4201 tatgcctacc gccggttcag cctggaaacc ggcccgatgc ctgctggcac ttaccaattc
     4261 gccagccggg caaccagcg
//
LOCUS       DASWQN010000916         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1142324, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000916 DASWQN010000000
VERSION     DASWQN010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142324"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..721
                     /locus_tag="VGC82_15630"
     CDS             251..721
                     /locus_tag="VGC82_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424750.1"
                     /translation="MKVLMCGLAALCMGGAVSASRAGDVYVVDNAKGKRPTVTQKDGF
                     TRNPVPVTNGGQNEGYSSAMMFTTPGTGVAVYTGHMEPGGKIAHHEGGTIYVLYVVQG
                     SGKLINLDPAGATTSEIAYKPDDVIVFQPNTHHQWENGPEAFDFTGVSQPQPKP"
     gene            complement(1322..1936)
                     /locus_tag="VGC82_15635"
     CDS             complement(1322..1936)
                     /locus_tag="VGC82_15635"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0424751.1"
                     /translation="MVLSSGRADLADADRRAADDRADAVVIRADMLQVQLEQLSIPCG
                     PDQNRRKLMRMSRRSLATFGFGGAIMLAVARVFRLALAADEESPAEAVEAFRRAMMTN
                     DQAEFNTLCAAQMSYGHSAGKIQTKDEFIADATSGKSTWKTLEFANLRNTVAGVNAIS
                     RFMLTGEVASDGKVTPVNIGVLMVWQKQEGAWKLLARQAFKVDK"
     gene            complement(1986..2123)
                     /locus_tag="VGC82_15640"
     CDS             complement(1986..2123)
                     /locus_tag="VGC82_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424752.1"
                     /translation="MAKSQKRSNREQKKPKQEKPKPTVGQSAFAGLHERPAVPTPTKK
                     K"
     gene            2257..>2498
                     /locus_tag="VGC82_15645"
     CDS             2257..>2498
                     /locus_tag="VGC82_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424753.1"
                     /translation="MADDLRLPAMGRIPCVGKFYRLFETSWRRDVLKPRAGAMVCLRL
                     KLRGGTKFCPSPRQDVRYGVTVSSLRYCSLPTNPAVP"
BASE COUNT          499 a          754 c          714 g          531 t
ORIGIN      
        1 acgtcctgca tttccggcag aagccaggtt attgcggatt cggccgtgaa aatggatgtt
       61 gtcgccgcat gagcctgcca tcaccacctc agtcccaccc gacttcacca ttcatgtcag
      121 cgggtggctg ttttgcctgc acagctggtc ggcgtaggct agtcccttct cacttgcagc
      181 ttggcacgat taccctcggg ttggcatatc ggtatgacat gctggtgacg cacgaacaca
      241 ggagcaaaca atgaaagtct tgatgtgtgg attggctgcg ctctgcatgg gtggcgccgt
      301 gtcggcgagc cgggcgggcg acgtctacgt ggtcgacaac gccaagggca aacggcccac
      361 ggtgacccag aaggacggtt ttacccgcaa tccggtgccg gttaccaacg gcggccaaaa
      421 cgaaggctat agttcggcga tgatgttcac cacacccgga acaggagttg cagtttatac
      481 cgggcacatg gaaccgggtg gcaagatcgc gcaccacgag ggcggaacga tttacgttct
      541 gtacgtggtg cagggatccg gcaaactgat caacctcgac ccggcaggcg cgacgacgtc
      601 ggagatcgcg tacaagcccg acgacgtgat cgtgtttcag ccgaacacgc atcatcaatg
      661 ggaaaacggg cctgaggcgt ttgactttac tggtgtgagc cagccgcagc cgaagccttg
      721 aggttagagg tggttctttg aaccaaaatt caagaacatt gcgatacgcg acactgtcgc
      781 gctcgattaa gtaaaggcgc acgcataacg ttccgcacta tgaaaacgcg ccttaaaacc
      841 gcgcgatggg gtaaacgaac gggatgcgcc ggcagcgcca tcctttcgcg ccgagcctgg
      901 tatcgtccac gccgcgtggc ggtaggaagt gcgggaccat gccgattggg tcgcttgatt
      961 gggagccgtg ctgccacccg ccggctgccg ccagctattc agccgccagg cccggaagaa
     1021 acgccgcgat cttcgcccgc cccacattcg ccatcggtat tccgtaagat ccaaatgcgt
     1081 ctacttgggc aagcggaccc agagtaagcc gatagggcca atgcacggct gcagccctgc
     1141 aacgggcacc tctgctctct aagtgtctga ccgaaagcat aatgaacgat ttcaactagt
     1201 tgcagaaagc ggattcgtaa agcgctctct gttcacggaa actccgctga atttctccac
     1261 atcatctaca aaattacgag caatttcaat gtccggtttc cggacagaca ctaaactaag
     1321 actacttgtc gaccttgaat gcttgccgtg cgaggagctt ccaggcgccc tcctgcttct
     1381 gccacaccat cagcacaccg atattgactg gcgtgacctt gccgtcgctc gccacttctc
     1441 cggtgagcat aaagcgggag atcgcgttga cgcctgccac ggtgttcctc agattggcaa
     1501 actcaagcgt cttccacgtt gacttcccgc tggtggcgtc ggctatgaac tcgtcctttg
     1561 tctgaatttt gccagcagag tgaccgtagc tcatttgcgc ggcacagagc gtgttgaatt
     1621 cagcctgatc gttggtcatc atcgccctgc ggaaagcctc gacggcctcc gcgggcgatt
     1681 cctcatccgc cgccaaggca agcctaaaga cccgggcgac ggcaagcatg atagcgccgc
     1741 caaaaccgaa tgtagcgagc gagcggcgag acattctcat cagtttcctc ctgttttggt
     1801 ctgggccgca cgggatgcta agctgctcga gctgaacttg cagcatatct gcgcgaatca
     1861 ccacagcatc ggcgcggtca tcggctgcgc gtcgatctgc atccgccagg tcggcccgac
     1921 ccgagctcag caccacgtca ccggcgccgg taggcaggtg gttgcgacaa ccggacgccc
     1981 gcttcttact tcttcttcgt gggcgttggt accgccggcc gctcgtgcag gccggcgaaa
     2041 gccgattgcc caacggttgg cttcggcttc tcctgctttg gcttcttctg ctcgcggttg
     2101 cttcgcttct gacttttggc cattaagctt ctcctggacc agtccgcatc gagcccagcc
     2161 gccttgggtg gggccaaagc aggatgcgcc ttcccgaagt gatatacgct tcacttcgaa
     2221 ggtgctggac accaccggcc aacggggtca cccgctttgg ccgatgatct gcggcttcct
     2281 gccatggggc ggattccctg cgtagggaag ttctaccggt tgtttgaaac gtcctggcgc
     2341 cgggacgttt tgaaacctcg tgccggcgcg atggtctgcc tgcgtttgaa acttcgcggc
     2401 ggcacgaagt tctgtccttc accccgccag gacgtgcgtt acggcgtaac ggtgtcgtcc
     2461 ttacgatact gctcccttcc aacaaatccg gcggttcc
//
LOCUS       DASWQN010000917         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_746116, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000917 DASWQN010000000
VERSION     DASWQN010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746116"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..733
                     /locus_tag="VGC82_15650"
     CDS             215..733
                     /locus_tag="VGC82_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY0424754.1"
                     /translation="MFDLFAGFFLVGVFGFGGVLPWARRMIVEQRKWLTAAEFTEMLG
                     LCGFLPGGNIMNVTIALGSRFHGIPGALSCFLGLMTAPVAIVIGLGAAYDQYAGLPPV
                     RRAFVALSAAAAALLLATAWKIVSPLRGRKLAIAVAACTFLAVAVLRLPLLAVMPVLA
                     TGSTLLLRRYRA"
     gene            730..1254
                     /locus_tag="VGC82_15655"
     CDS             730..1254
                     /locus_tag="VGC82_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY0424755.1"
                     /translation="MNTLLDLAAVYGKLSLLSFGGANAVIPEMQRQVVDVHHWMNARE
                     FAALYALAQAAPGPNMMVVSLVGWRVAGFWGALVTTGAVAVPSSVLTLLVSGLWLRFK
                     DANWRKAVQAGLQPVTAGLIMSSAVLLIETTAVDLTAIAVTAVSAALFIFTKLHPMLI
                     LATAAAAGAIGLVG"
     gene            1587..2405
                     /locus_tag="VGC82_15660"
     CDS             1587..2405
                     /locus_tag="VGC82_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424756.1"
                     /translation="MVKHTLNNGQSITVAADQWDGSLLAFAGTSNAFGQTPTAVFTGP
                     NTLASIADIGSPTVHGQGAINLMPGSALTTGGLTVNAATLYISERPGTSMTLNGTSTI
                     ANGSTLTATGYAGAGIYIVNGRMNIDATSTVYMDYVAVKGTGTIHLTGNALLRAGVVS
                     STSETVVLDGGMLSLTNGMNFQGTITSSTPASSRIGALASVDVYNAMGAVRETFNRTT
                     GMLGLFNAQGGQVASLKFAGSGDLYAAPTTGLPTNYMAITSHPSVGALHVAFSA"
     gene            complement(2524..>2823)
                     /locus_tag="VGC82_15665"
     CDS             complement(2524..>2823)
                     /locus_tag="VGC82_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEY0424757.1"
                     /translation="AGRTMAFVGIGLGFIFAVVALTISAVSFPMLLDQNVSLETAVRT
                     SAAVVMRNPLVMATWGLIVAGSLVAGSIPLLLGLVVVLPVLGHATWHLYRRAVSV"
BASE COUNT          540 a          905 c          816 g          562 t
ORIGIN      
        1 gcggaataat ccgggccagc agcaagaaag ctgagccaat gttcgcggtc accctggtac
       61 tcctgtttat cggcatagtg ctatgccgac gcttgatgcg ggccaacgcc ccttcggaac
      121 ctcagtcttc cacagatcac ctcccggcga ttgtccgaat tgactgctgt cgctaattca
      181 ttgccgcatg tccgaaacgc ggcccgctcc aacattgttc gacctcttcg ctggcttttt
      241 cctggtcggt gtcttcggct tcggcggcgt cctgccctgg gcgaggcgta tgatcgtcga
      301 acagcgcaaa tggctcaccg ccgccgagtt caccgagatg ctcgggctct gcgggttcct
      361 gcccggcggc aacatcatga acgtgacgat cgctctgggg tcgagatttc acggcatccc
      421 cggggccctt tcctgcttcc tgggcctgat gacggcccct gtggcgatcg tgatcggcct
      481 cggcgcggcc tacgatcagt acgccggcct tcccccggta cgccgcgcct ttgtcgcgct
      541 gtccgccgcc gcggccgccc ttctgctggc aaccgcctgg aagatcgtct cgccgttgcg
      601 aggccggaag ctggcgatcg ccgtcgctgc ctgcaccttt ctcgcggtcg ccgtgctgcg
      661 gctgccactg ctggcggtga tgccggtgct ggcgaccggc tccacgcttc tgctacggcg
      721 gtaccgcgcg tgaacacgct gctcgatctc gccgcggtct atggcaagct ctcgcttctg
      781 tcgttcggtg gagcgaacgc ggtgatcccc gaaatgcagc gtcaggtcgt cgatgttcac
      841 cactggatga acgcgcggga gttcgccgcc ctttatgccc tggcgcaggc cgcgccgggg
      901 cccaacatga tggtggtcag cctggtcggc tggcgggtcg cggggttctg gggcgcactg
      961 gtgaccacgg gggcggtcgc ggtgccttcg tcagtgttga cgctgctggt cagcggcctg
     1021 tggctgcgct tcaaggacgc gaactggcgc aaggcagtcc aggcgggact gcaaccggtg
     1081 acggcagggc tgatcatgtc cagcgccgtg ctgttgatcg aaaccactgc ggttgacttg
     1141 accgccatcg cggtcacggc cgtgtcggcc gcgctgttca tcttcaccaa gctgcatccg
     1201 atgctgatcc ttgccactgc cgctgccgcc ggtgcgattg gcttggtcgg ataacgatta
     1261 cgatatcaga taatcatcga gctttccact gacgtccaaa ccggatggtt gatgttactt
     1321 tttgtacaag atcacgatta cgtacatcaa aaagtgtaga taacggaatg gattatccga
     1381 atttaatact ttttcgttat ataattgcgg ttgttatggc gttacagcgt ccattttccg
     1441 attctctaat cctaatccga tacaataaca ggcgccattt tcattacgat tcaagtcgaa
     1501 gaaatcgtcc acgttttcgt gaaatctact ttcacagccg ttacgttatt cgatcataac
     1561 gctttgcatc ccgaggagaa aagaccatgg tcaagcacac attgaacaac ggccagtcca
     1621 tcaccgttgc agccgatcaa tgggacggaa gcctgttggc attcgcggga accagcaacg
     1681 cattcggcca gacgccaaca gcagtcttca ccggccccaa tacgctcgcc agcatcgccg
     1741 acatcggatc gccgacggtc catggtcaag gtgcgatcaa cctgatgcca ggaagcgcgc
     1801 taacgaccgg cggcctgacc gtcaacgctg ccacattgta catcagtgaa cgtcccggca
     1861 ccagcatgac cctgaacgga accagcacga tcgccaatgg cagcacgctg accgcgacag
     1921 gctatgccgg cgccggcatc tacatcgtca acggcaggat gaacatcgat gcgacatcta
     1981 ccgtctacat ggactatgtt gcggtaaagg gcaccggaac gatccatctc acgggcaatg
     2041 ccttgctgcg ggccggcgtc gtcagcagca ccagcgagac cgtcgtgctg gacggcggca
     2101 tgcttagcct gaccaacggg atgaacttcc aaggcacgat cacgagttcc acgcccgcgt
     2161 catcgcggat cggcgcgctt gcctccgtgg atgtgtacaa cgcgatgggc gcggtgcggg
     2221 aaacgttcaa ccgcaccacc ggcatgctag ggctgttcaa cgctcaagga ggccaggtcg
     2281 ccagcctgaa atttgccggc agcggtgacc tttatgcggc accaacgacc ggcctgccga
     2341 cgaactatat ggcgatcacg tcacacccgt ccgtcggggc gctgcacgtc gcgttctcag
     2401 cctaatcaag ggcgttccgg ctcagcctaa ggcggtccgg gaaattcgca tgcggcgggc
     2461 tttcgggcac aaaagcccga tcccgccgcc ttgtcttcgg cgctaaccgc cttgtcttcg
     2521 gcgctaaacg gaaaccgccc gccggtacag atgccatgtc gcgtgcccca gcacaggcaa
     2581 aacgaccacc aggcccagca acagcggaat cgacccggcc accaggctgc cggcgacgat
     2641 caacccccat gtcgccatca ccagcggatt gcgcatgacg acagcagccg atgttcgcac
     2701 cgctgtctcc agcgacacgt tctggtccaa cagcatcgga aacgacactg cgctgatggt
     2761 cagcgcgacg accgcgaata tgaagcccag gccgataccg acgaacgcca tcgtccggcc
     2821 ggc
//
LOCUS       DASWQN010000918         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_352214, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000918 DASWQN010000000
VERSION     DASWQN010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352214"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..689)
                     /gene="ruvX"
                     /locus_tag="VGC82_15670"
     CDS             complement(219..689)
                     /gene="ruvX"
                     /locus_tag="VGC82_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY0424758.1"
                     /translation="MALFNLKDLLASLGQDQRVIGIDPGSRIIGLALSDVRRTLASPY
                     GSLKRGKLTANATEIARIALREGAYGLVIGLPLSMDGTAGPAAQAARDWAHALSAATG
                     LPAALWDERLSSAAVNRFLINEADLTRAKRAEAVDRAAAAWMLQAALDAAGVQG"
     gene            737..1024
                     /gene="gatC"
                     /locus_tag="VGC82_15675"
     CDS             737..1024
                     /gene="gatC"
                     /locus_tag="VGC82_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY0424759.1"
                     /translation="MSLDHATIRRIASLARIRVEEHEVEDLCHELNGILGWIEQLNEV
                     DVTGVAPLSGAVGMAMAMRADVVTDGGYADKVLSNAPERIGDFYVVPKVVE"
     gene            1024..>2178
                     /locus_tag="VGC82_15680"
     CDS             1024..>2178
                     /locus_tag="VGC82_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0424760.1"
                     /translation="MLTDLTIVQARDGLRTKQFSAVDLTMAHLHAIEALNPRLNAFIT
                     VSHAQALDQAREADAALAAGQARALTGIPLAIKDLFCTQGVRTTAGSKILGPFVPPYE
                     STVTANLLRDGAVFLGKANLDEFAMGSSNMTSAYGPVENPWKRKQDATAVLVPGGSSG
                     GSAASVAARLAMGSTGTDTGGSIRQPASFCGLVGVKPTYGRCSRWGVVAFASSLDHPG
                     PLARTVRDGAILLGSMAGHDPKDSTSASVPVPDFEAACARGVRGLRIGVPKEYRAEGM
                     PAEIEALWQQGLDWLRAAGAEIVDVSLPHTKYGLATYYIVAPAEASSNLARYDGVRFG
                     ARENGEDLRDLYERTRAEGFGAEVRRRVLIGTYVLSAGYYDAYYLRAQKIR"
BASE COUNT          352 a          707 c          743 g          376 t
ORIGIN      
        1 ttgggcatgg gattcgccca tgcgcagtct cgtcggttgc cgtgcgttgc cgaagcgggc
       61 gtaggtatcc attgctggtg cgaattcggc gtccgcgaca ggaaccatcc ggaaccccag
      121 tgatcctaaa aacaaccgga cgaggtcgcg cgactttcga ccggcagtcg atatacgtgg
      181 gcacgaccaa cggctgtctc ccacgacgcc tggctgattt acccctgcac gccagcagca
      241 tccagcgccg cctgcagcat ccaggcagcc gccgcgcgat cgaccgcctc ggctcgcttg
      301 gcgcgggtca ggtcggcttc gttgatcaga aaccggttca ccgccgcgct ggacagccgc
      361 tcatcccaca gcgccgcggg cagcccggtc gcggcggaca gcgcatgtgc ccaatcccgg
      421 gcggcctggg cggctgggcc ggcggtgccg tccatcgaca gcgggaggcc gattaccagg
      481 ccgtaggccc cctcccgcag cgcgatcctg gcgatctcgg tcgcgttggc ggtcagcttc
      541 ccccgcttca ggctgccata gggcgacgcc aaggttcgcc ggacatcgga caacgccaga
      601 ccaatgatcc ggctgcccgg atcgatgcca atcacacgct ggtcctgccc cagcgaggcc
      661 agcagatcct tcaggttgaa cagagccatg cccaccgtta tggtcccggc cttccattca
      721 cccaaggatt ctcttcatgt cgctcgatca cgcgaccatc aggcgcatcg cctcgctcgc
      781 ccgcatccgc gtggaggagc acgaggtcga agatctgtgc cacgaactga acggcatcct
      841 gggttggatc gagcagttga acgaggtcga tgtgactggc gttgcgccgc tcagcggggc
      901 cgtcggcatg gcaatggcga tgcgtgccga tgtcgtcacc gatggcggct acgccgacaa
      961 ggtgctgtcc aacgcaccgg agcggatcgg cgacttctac gtcgtgccga aagtcgtcga
     1021 gtgatgctga cggatctgac gatcgtccag gcccgtgacg ggctgcgtac gaaacagttc
     1081 tccgccgtcg atctgacgat ggcgcatctg catgcgatcg aggctctgaa ccccaggctg
     1141 aacgccttca tcaccgtcag ccacgcccaa gccctcgatc aggctcgtga ggccgatgcg
     1201 gcgctggcgg cgggccaggc gcgggcgctg accgggattc cgctggcgat caaggacctg
     1261 ttctgcacgc agggggtgcg cacgacggca ggcagcaaga tcctcgggcc gttcgtgccg
     1321 ccgtatgaga gcaccgtcac cgccaacctg ctgcgcgacg gcgcggtgtt cctgggcaag
     1381 gcgaaccttg atgagttcgc gatggggtcg tcgaacatga cctcggccta cggcccggtg
     1441 gagaacccct ggaagcgtaa acaggatgca accgcggtgc tggtcccggg cggctcgtcc
     1501 ggcgggtcgg ccgcttccgt ggcggctcgg ttggcgatgg gatcgaccgg aacggacacg
     1561 ggcggttcga tccgtcagcc ggcgtcgttt tgcggcttgg tgggggtgaa gccgacctat
     1621 gggcgctgtt cgcggtgggg cgtggtggcg ttcgcgtctt cgctggatca ccccggaccg
     1681 ctcgcacgga cggtgcggga cggcgcgatc ctgctgggtt cgatggccgg ccacgatcct
     1741 aaggactcca ccagcgcttc tgtcccggtg ccggatttcg aggcggcctg cgcccgcggc
     1801 gtgcgaggcc tgcgcatcgg cgtgccgaag gagtaccgcg ccgaaggaat gccggcggag
     1861 atcgaggcgc tgtggcagca aggcctggac tggcttcgcg ccgccggagc ggagatcgtc
     1921 gatgtttccc tgccccacac gaaatatggc ctggcgacct actacattgt ggctccggcc
     1981 gaggcgtcct cgaacctcgc gcgctacgat ggggtgcgtt tcggcgcacg cgaaaacggc
     2041 gaggatctgc gcgatcttta tgagcgcacc cgagccgagg gattcggcgc ggaggtgcgc
     2101 cgccgtgttt tgatcggcac ctatgtgctg tcggctgggt attacgacgc gtactacttg
     2161 cgggcgcaga aaatccgg
//
LOCUS       DASWQN010000919         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1538434, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000919 DASWQN010000000
VERSION     DASWQN010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538434"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VGC82_15685"
     CDS             complement(<1..956)
                     /locus_tag="VGC82_15685"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0424761.1"
                     /translation="MIDVQALEGCKVLDVSTFLAGPFCATQLGEFGADVIKVELPKVG
                     DATRRFGTMTDCGDSLPWMSESRNKRCITLDLRKPEGAALLKRLVAQSDIMVENFQPG
                     TLEKWGLGYDVLSAVNPRLIMVRISGYGQTGPYKDRPGFGRIGNAFGGLSFLAGYPDR
                     APVTPGSATIPDYMAGLYGALGSLLALQAREKTGRGQVVDIGLYEPIFRILDELAPAF
                     AYKGYVRQRMGPGTVNVVPHSHYPTKDARWIAIACTNDKIFARLAEAMGRADLAEGGS
                     WGTLAARERDRALVDETVGAWTATMDRADLMAACERFQVPCGP"
     gene            complement(953..1798)
                     /locus_tag="VGC82_15690"
     CDS             complement(953..1798)
                     /locus_tag="VGC82_15690"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY0424762.1"
                     /translation="MRSLLFAPGNHARRVEKALGLPADGVILDLEDAVAVSEKTATRP
                     IVTEAFGRPRAGKLYVRVNALTTEWCYGDIGAVVQGGLDGIILPKVEHAHELRTAEWL
                     IANLERERDLAAGAIDLIPIIETALGISNLTSICRSGTRTKRLAFGAGDFTLDMGMVW
                     SRSEAELLPYRSACVLESRAAGLEPPIDTVWADLRDSDGFIASARHAAQIGFQGKMCI
                     HPDQITAANAAFSPDEATVAQARRVVEAFDKAEALGLASIQLDGQFIDYPIVQRARRV
                     LGAAR"
     gene            complement(1795..>2112)
                     /locus_tag="VGC82_15695"
     CDS             complement(1795..>2112)
                     /locus_tag="VGC82_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0424763.1"
                     /translation="KNPTVRDFVEVSGRGTIAEHKVFCGTPKSVADQMEEWFTAPACD
                     GFVLAATHMPGAYEDVVRLVVPELQRRGLFQKDYRGSTLRENLGLPIPQAADWRRRRG
                     DML"
BASE COUNT          386 a          676 c          673 g          377 t
ORIGIN      
        1 gggccgcagg gtacctggaa ccgctcgcaa gcggccatca gatccgctcg gtccatcgta
       61 gcggtccatg cgccgacggt ttcatcgaca agggcacgat cgcgttcgcg cgcggccagc
      121 gttccccagc ttccaccttc cgcgagatcg gcgcgcccca tcgcctcggc gaggcgggcg
      181 aagatcttgt cattggtgca tgcgatcgcg atccagcgtg cgtctttggt tgggtagtgg
      241 ctgtgcggca cgacgttgac ggttccaggc cccatacgct gccggacgta ccctttgtaa
      301 gcaaaggccg gggccagttc gtcaagaatg cggaagatgg gttcatacag accgatatcg
      361 accacctggc cgcgcccggt tttctcccgc gcctgcagcg ccaacaggga tcccaaggcg
      421 ccgtacaacc ccgccatata gtcgggaatt gttgccgatc caggcgtgac cggcgctcga
      481 tccggatagc cggccaggaa ggacaggccg ccgaacgcat tgccgatgcg accaaacccg
      541 ggccgatcct tgtatggacc ggtctggccg tagccggaga tgcgcaccat gatcagccgt
      601 ggattgaccg cgctcaggac gtcgtagccc agcccccatt tttcgagcgt gccgggctga
      661 aagttctcga ccatgatgtc ggactgcgcg accaggcgtt tcagcagcgc ggcgccttcg
      721 ggcttgcgca aatccagggt gatgcagcgt ttgttgcggg attccgacat ccacggcagc
      781 gagtcgccgc agtcggtcat cgtgccaaag cggcgggtgg catcgccgac cttgggaagt
      841 tcgaccttga tcacgtccgc gccgaactca cccagctgcg tggcgcagaa cgggccggcc
      901 aggaacgtcg agacatccag gaccttgcag ccttccaggg cctgcacatc gatcatcttg
      961 cggctccaag cacgcggcgg gcgcgttgca cgatgggata gtcgatgaac tggccatcga
     1021 gttgaatcga cgccaatcca agcgcttcgg ctttatcgaa cgcctcgaca acgcgcctgg
     1081 cctgggcgac ggtggcctca tcaggcgaaa acgctgcgtt ggcggcggtg atttggtcgg
     1141 gatggatgca catcttgccc tggaacccaa tctgcgcggc atgacgggcg gaggcgatga
     1201 aaccgtcgct atcgcgcaaa tccgcccaga ccgtgtcaat cggcggttcc agcccggcgg
     1261 cgcgagactc cagcacgcag gcgcttcgat acggcagcaa ttcggcttca gaccgtgacc
     1321 agaccatccc catatccagg gtgaaatcgc cggcaccgaa agccaggcgt ttcgtgcggg
     1381 tgccggaccg gcagatggat gtcagattgc tgatgcccag cgcggtttcg atgatcggga
     1441 tcaggtcgat ggcaccggcg gcaagatcgc gctcccgctc cagattggcg atcagccact
     1501 cggcggtgcg caattcatgg gcatgctcga ccttgggcag aatgattccg tccaggccgc
     1561 cctgaacgac ggcgccgata tcgccatagc accattccgt ggtcagcgcg ttgacccgca
     1621 catacagctt gccggcccgc ggcctgccga aggcttccgt cacgataggt cgggtcgcag
     1681 tcttttcgct gacggcgaca gcatcttcca gatccaggat gacgccgtcg gcgggaagac
     1741 cgagcgcctt ctcgacgcgg cgtgcatgat tgccgggggc aaacagaagg cttctcatag
     1801 catgtccccc cggcgccgac gccagtccgc tgcctggggg atcggcaggc ctaggttctc
     1861 gcgcagcgtg ctgccgcgat agtccttctg aaacagaccg cgacgttgca gttccgggac
     1921 caccaggcgc acgacatcct cataggcgcc cggcatgtgc gtcgcggcca gaacgaagcc
     1981 gtcgcaggca ggtgcggtga accattcctc catctgatcg gcgacagact tcggcgtgcc
     2041 acagaatact ttgtgttcgg cgatggtgcc gcggccggat acttcgacga agtcgcggac
     2101 cgtgggattc tt
//
LOCUS       DASWQN010000920         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2462423, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000920 DASWQN010000000
VERSION     DASWQN010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2462423"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VGC82_15700"
     CDS             complement(<1..320)
                     /locus_tag="VGC82_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424764.1"
                     /translation="MTITLLTQDQRDINQFSMNEARHLGVYHGHGVFLRRKYYTCLSQ
                     ISEANLAGPDPTSDQGADNFRWRRQPNVALFNTQPVCPPSADQESPVGLLDFTVTENR
                     VLCS"
     gene            359..2431
                     /locus_tag="VGC82_15705"
     CDS             359..2431
                     /locus_tag="VGC82_15705"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY0424765.1"
                     /translation="MRQINRVFAAVVILTMLLFSPNSQAVDWAPWLWANFCGQLPKEM
                     VSRAAYSLIAPANTQTVPCAAAPTGYHRLQQAASDGDLAVDLFVGTDPAGSSLCYAYN
                     GVPEAPVIRIQRGHTLTVRLTNTLNDTGPDNTRNCLLQTFVDGGACDEPEQGFRAQPG
                     MDDGFYPIQSNVPHLADGSTNLHVHGFVVSPRPCHDEVIRSKIYPANWGGPVTGLLPC
                     QAAPNELTYTYDIPADHPEGLYWYHTHRHGQAQAATMLGLVGAIVIEGPDDTRRAAMG
                     VGDDVLVIHDVPIMQPGSPPQPMASHKSVLNRQKRHAKVQPVADPRIDQANEVSCEPG
                     DPDTGGPQVTALTLNGASVPEMPDGSFPPDNTVLTKTMRPGQTEIWRILNASANTAIR
                     PRLSMVRDGVTSMLPLVVLARDGVPVADDAGYPSMQTIDTTNKPILLDPANRLEIVVH
                     APPPGATLYLDTAQVAVGCAGDGDPARRLLRVVSTGAPMTAPVPDAALAPADPDMYYT
                     HMLDQVPAVQRVLAFTEYPRGFTVARSTWIGQAPQPGQFDPDVTDFYLNQIESSDDPT
                     FAPVIKPFDMHTLRPDLVVHLHGGEKTTEQWTIQNYSLEFHAFHIHQVHFRDISAGSR
                     DPFDAPLLDTVNVPPAGNNGGMAGAPGQTTIVMTFTKAQIGEFVFHCHILEHEDNGMM
                     QKIRVVAD"
     gene            2632..>3018
                     /locus_tag="VGC82_15710"
     CDS             2632..>3018
                     /locus_tag="VGC82_15710"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424766.1"
                     /translation="MLTTGRRTLARIGMAAQAMAVLPRGLRAADAPVRIGAPYPLTGV
                     AASAGMSVKQAIEVAADIINNAHPELPNLPLAKTEGLPGLGGRKVEVIFADHQGNPAI
                     AQSEALRLITQDKVTAMVGCYQSSCGL"
BASE COUNT          606 a          981 c          895 g          536 t
ORIGIN      
        1 caactacaaa ggaccctgtt ttcagtcacc gtgaaatcga gcaagccgac tggactttct
       61 tgatctgccg agggcggaca tactggctgc gtgttgaaca gggcgacgtt tggctgcctg
      121 cgccaccgaa aattatccgc cccttggtcg ctcgtcggat cggggccggc cagattcgct
      181 tcgctgatct ggcttagaca agtataatac tttctccgta agaatacgcc atggccatga
      241 taaacgccca aatgtcgagc ttcattcatt gaaaattgat taatatcacg ctgatcctgc
      301 gttagaagcg taatcgtcat tcggatcaac gtcgccgcga acgtataggc aaacgacaat
      361 gcgccagatc aatcgagtgt ttgccgccgt ggtcatcctg acgatgctgc tgttttcgcc
      421 caattcccag gccgttgatt gggcaccgtg gctctgggct aatttctgcg gccagttgcc
      481 gaaggaaatg gtctcaaggg cggcctacag cctaatcgct ccggcgaaca cacaaactgt
      541 cccctgcgcc gccgcgccaa cgggctatca tcgcctgcaa caggcggcca gcgatggcga
      601 ccttgcggtc gatctttttg tcggaaccga tcccgccggc tcgtccttgt gttacgccta
      661 taacggcgtg cccgaagcgc cggttatccg aatccagcgg ggccacacct tgacggtgcg
      721 gttgaccaat acgctgaacg acaccggacc cgacaacacg cggaactgcc tgcttcagac
      781 gttcgtcgat ggcggcgcct gcgacgaacc cgagcagggc ttccgcgccc agccgggaat
      841 ggacgacggc ttctatccga tccaaagcaa cgtgccacat ctggcggatg gttcgaccaa
      901 cctgcacgtc catggctttg tcgtatcgcc acgcccatgc catgacgaag tcatccgcag
      961 caagatctat cccgcgaatt ggggtggacc ggtgacaggt ttgctcccct gccaggcggc
     1021 tccgaacgag ctgacctata cctacgacat accggccgat cacccggaag ggctgtactg
     1081 gtatcacacc catcgccacg gacaggccca ggcggccacc atgctcggtc ttgtcggtgc
     1141 gatcgtgatc gaagggccgg acgacacacg ccgcgcggca atgggcgtgg gcgacgacgt
     1201 gctggtgatc cacgacgtcc cgatcatgca gcccgggtcg ccgccgcaac cgatggcgtc
     1261 gcataaatcc gttctcaacc ggcagaaacg gcatgccaaa gtgcagccgg tcgcagatcc
     1321 ccgtatcgat caagccaacg aggttagctg cgagcccggc gaccccgaca ccggcggtcc
     1381 gcaggtgact gccctgacct tgaacggcgc gagtgttccg gagatgccgg atggctcctt
     1441 tccgcccgac aacacggtcc tgacaaagac catgcggccc gggcagacag agatctggcg
     1501 catcttgaac gcgtccgcca acacggcgat ccgcccacga ctatcgatgg tgcgggacgg
     1561 ggtcaccagc atgctgccat tggtggtgtt ggcgcgagac ggcgtcccgg tcgcagacga
     1621 tgcgggctat ccgtcgatgc agacgatcga cacgacaaat aaaccgattc tgctggaccc
     1681 cgccaaccgg ttggagatcg tcgttcatgc tccgcctccc ggcgcgacgc tatatctgga
     1741 taccgctcag gtcgcggtcg gctgcgccgg cgacggcgac ccagcgcgac ggctgttacg
     1801 ggtggtatcg accggcgcgc cgatgacggc tccggtgccg gatgccgcgc tggctccggc
     1861 cgaccccgat atgtattata cgcacatgct ggatcaggtg ccggcggtgc agcgcgtact
     1921 cgctttcacg gaatatccgc gcggctttac cgtcgcccgg tcaacgtgga tcggtcaggc
     1981 gccgcagccg ggacaattcg atccggacgt gacggacttt tacctgaacc agatcgaatc
     2041 aagcgatgac ccgacatttg ccccggtcat caagccgttc gacatgcaca cgctccgccc
     2101 cgatctcgtc gtgcatctgc atggcgggga gaaaacgacg gagcaatgga cgatccaaaa
     2161 ctattcgctg gagtttcatg cgttccacat ccatcaggtc catttccggg acatcagcgc
     2221 gggcagccgc gatccgttcg acgcgccgct tctggacacg gtgaacgtgc cgcccgccgg
     2281 caataacggc ggcatggcgg gcgcgccggg acaaacgacg atcgtcatga cgttcaccaa
     2341 ggcgcagatc ggcgaattcg tgttccactg ccacatactc gaacacgaag acaacgggat
     2401 gatgcagaaa atccgcgtgg tcgccgactg agccagagcg ttatttcgaa ctgaagatac
     2461 cccggcttcc aacgccggcc aaccgcaggg caaactttgc caggcgcttc aattaccccg
     2521 caagaggcgg tcatcgaccg tccgcaccaa ccttcggatc gtgccggccg gacccattgt
     2581 cgggcgaccc gggtgcctgt gcactgtgcg gccttgcagg agggaaacga gatgctgacg
     2641 actggccgta gaaccctggc ccggatcggg atggccgcac aagccatggc cgtgttgccg
     2701 cgtggcctgc gcgccgccga cgcccccgtc aggatcggcg ctccgtaccc actgaccggc
     2761 gtcgctgcct cggccggcat gtcggtcaag caggcgatcg aagtcgccgc cgacatcatc
     2821 aacaacgcgc acccagagct gccaaacctg cccttggcca agaccgaggg cctgcccgga
     2881 cttggcggcc gcaaggtcga ggtgatcttc gccgaccatc agggcaaccc ggcgatcgcg
     2941 caaagcgagg cattgcgcct gatcacccag gacaaagtca ccgccatggt gggctgctac
     3001 cagtcgagct gcggcctg
//
LOCUS       DASWQN010000921         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1011607, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000921 DASWQN010000000
VERSION     DASWQN010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011607"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1521
                     /locus_tag="VGC82_15715"
     CDS             115..1521
                     /locus_tag="VGC82_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424767.1"
                     /translation="MENQQAIKPATATGIKPTHVRWGILAMLFVITTINYADRATISL
                     AGPELKRVLGLTPVEMGYVFSAFAWSYVACQLPGGWLLDRFGSKITYFFSIFFWSLLT
                     VLMGTVGFFTGATAVALLFGLRLAIGAAESPSFPGNSRITSAWFPTAERGIASAIFNS
                     AQYFATVLFAPLMGWLVHTFGWQSVFYVMGGLGIVLSFVWLKVIYGPKTHPRINAAEL
                     AYIDTGGALVDLEGTTDKIEPKKVNSVAIIKELLSNRMLLGIYIAQYCITTLTYFFLT
                     WFPVYLVQERHMTILKAGFIAALPAIAGFLGGILGGIISDQMIKRGYSLSAARKTPIV
                     AGMVLAMSMILCNYVSADWLVVAIMALAFFGKGVGALGWAVVSDTSPKEAGGLSGAVF
                     NTFGNVAGITTPIVIGYILQATGSFAGALIYIGANAAAAIFCYLVIVGPIKRFSFQET
                     PVQPGMIAPPTPAHALGK"
     gene            1531..>1979
                     /locus_tag="VGC82_15720"
     CDS             1531..>1979
                     /locus_tag="VGC82_15720"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEY0424768.1"
                     /translation="MRDSIHTPAPKESVITPTDSVHDRPPVKPLYIRMHANDNVAIVA
                     NDGGLPAGTVLSSGQTLRDHVPQGHKVALIDFEVDAPVLRYNVIIGRALKPIPAGSWV
                     HERLLAMPPARELEGLPIATIKPETMPPLTGYTFQGFRNPDGTVGTR"
BASE COUNT          367 a          622 c          592 g          398 t
ORIGIN      
        1 accgggttat cgcaatcgtg gagacggcaa gaacttgagt cgcacccaga agcggccagc
       61 ctatcggggt tcgaagtgcg ggtaacacca ttcgaagtaa tttgaggaga cagtatggag
      121 aaccagcaag cgatcaaacc agccaccgca accgggatca agccgaccca tgttcgatgg
      181 ggaattctgg cgatgctgtt cgtcatcacc accatcaact atgccgaccg cgcgaccatc
      241 tcgcttgccg ggccggagct gaaacgagtg ctgggtttga cgcccgtaga gatgggttac
      301 gtcttttcgg cgttcgcctg gtcgtacgtg gcgtgccagt tgccgggcgg ctggctgctc
      361 gaccgtttcg gctccaagat cacgtatttc ttcagcatct tcttctggtc gctgcttacc
      421 gtgctgatgg gcacagtcgg ttttttcacc ggtgccaccg cggtcgcact cctgtttggc
      481 ctgcggcttg cgatcggggc ggccgaatcg ccctcttttc ccggcaacag ccgcatcacc
      541 tcggcctggt ttccgaccgc tgagcgcggc atcgcatcag ccatcttcaa ttcggcgcag
      601 tatttcgcca ccgtgctgtt cgccccgctg atgggctggc tggtgcacac gttcggttgg
      661 caaagcgtgt tttacgtgat gggcggcctg ggcatcgtgc tgtctttcgt ctggctcaaa
      721 gttatttacg gaccgaaaac gcacccgcgc atcaacgcgg ccgagctggc ctatatcgat
      781 acgggcggcg cgctggtcga tctggaaggc acgaccgaca agatcgagcc gaagaaagtc
      841 aatagcgtcg ccatcatcaa ggaactgttg agcaaccgca tgctgctggg catctacatc
      901 gcccagtatt gcatcacgac gctgacctat ttcttcctga cctggtttcc ggtctatctg
      961 gtgcaggaac gccacatgac gatcctcaaa gccggcttca tcgccgcgtt gccggccata
     1021 gccggctttc tcggcggcat tctgggcggc atcatttcgg accagatgat caagcgcggt
     1081 tactctctgt cggcggcgcg caagacgccg atcgtggcgg gcatggtgct cgcgatgagc
     1141 atgatcctgt gcaactacgt ctcggccgac tggctggtgg ttgccatcat ggcactggcg
     1201 ttcttcggca aaggcgtcgg cgccctgggc tgggcggtgg tgtccgatac gtcgccgaaa
     1261 gaggccggtg ggctgagcgg agccgtgttc aataccttcg gcaatgtggc cggcatcacc
     1321 actccgatcg tgattggcta tatcctgcag gcgaccggct cgttcgccgg cgcgctgatc
     1381 tacatcggcg ccaacgcggc agccgcgatc ttctgttacc tggtcatcgt cggaccgatc
     1441 aaacgtttct cattccagga aacgccggtc cagcccggca tgatagcgcc tccaacacca
     1501 gcgcatgctc taggcaaata aaggaaagag atgagagact ccattcatac cccggcgccc
     1561 aaagaatccg ttattacgcc gacggactcc gttcacgacc gaccgccggt caaaccgctg
     1621 tacatccgca tgcatgccaa cgacaacgtc gcgatcgtcg ccaacgacgg cgggctgccg
     1681 gcgggcacgg tgctgtcttc gggccagacg ctgcgcgacc acgtgccgca gggccacaag
     1741 gtggcgctga tcgatttcga agtagacgcg ccggtgctgc gctacaacgt gatcatcggt
     1801 cgcgccctga agcctattcc cgccggcagc tgggttcacg agcgcctgct ggcgatgccg
     1861 ccggcgcgcg aactcgaagg gctgccgatc gccacgatca agcccgagac gatgccgccg
     1921 ctcaccggct acaccttcca gggcttccgc aacccggacg gcacggtcgg cacgcgcaa
//
LOCUS       DASWQN010000922         5321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2068014, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000922 DASWQN010000000
VERSION     DASWQN010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068014"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1184)
                     /locus_tag="VGC82_15725"
     CDS             complement(318..1184)
                     /locus_tag="VGC82_15725"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0424769.1"
                     /translation="MSDFELVWDSRCGVAESPVWDAATRRLLFCDINGKRINALTVDT
                     GARQSWDFPEVVGSFGLCRSGRLVVAQRHHVVLFDPASGALTDLTPKIDEPATNRLND
                     GKVGPDGAFWVGSMDENSPRQKIAGLYRVTSDGRITRQADGYAVSNGLAWSPDGRTMY
                     HSDSTAGIIEVWDFDPTTGDRGNHRILATLTNDDGRPDGAATDMDGNYWSAGPSAGCL
                     NCFSPAGKLLRKWAFPVPGPTMPCFADGSLYVTSLREGKSAQVLADHPALGGLFRAAA
                     PAAGAPVGLFED"
     gene            1326..1994
                     /locus_tag="VGC82_15730"
     CDS             1326..1994
                     /locus_tag="VGC82_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0424770.1"
                     /translation="MDKIEAVFGDHAQLAHQLLSDLGPDGDDGSHDRSHLLRVWRNAA
                     DIAARESGCDWAVLTAAVILHDCVAVEKNSPQRSQASRLAAARARTILAGLAWSPAII
                     DRVAHAIEAHSFSAGIAPETMEARVVQDADRLDAIGAIGIARCFYVAGRMGSGLYDPD
                     DPAAANRPLDDRNFALDHFGAKLFHVAGNFQTKAGQALADARTRTMHDFVRAMLNEIG
                     KASE"
     gene            1991..2989
                     /locus_tag="VGC82_15735"
     CDS             1991..2989
                     /locus_tag="VGC82_15735"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0424771.1"
                     /translation="MIRAAIVGLGWWGQNLVNSVRGSSAIRFTTAHTRTRTTVAGFCS
                     EAGLRWVDSLDEILNDNEINAVVFATPHTQHVDQVVRAAGAGKHVFVEKPFGLSVEDA
                     IRARDAADRAGIVLAVGFNRRFHPSMGMLRTAVTSGRLGTLVTISAEQTALHGITMMP
                     EAWRAQPTEAPAGAMTAIGVHLVDGMIDLFGPIRQVYAQVARRAAPLADDTTDVMLRF
                     ANGASGHIFCSTAATPHYRMAVYGTKGFAEVLGHPMTTFRLIPAGDGHPAAGTPEVTE
                     TTGFNMLNAELEAFAASIVARQPFPTPLREIMHGVAVFEAIAASASTGQPVEVPPV"
     gene            3110..4039
                     /locus_tag="VGC82_15740"
     CDS             3110..4039
                     /locus_tag="VGC82_15740"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0424772.1"
                     /translation="MLALAARPAWAQPISAGKPSPFAPGDLKRPIFDTAKPVYDTANH
                     QERSASSVVAEVDGRAITLGEVGDAIKELPASVQHLPFADLFPAVLTQLVRQQALVIR
                     SQHQALDEEPTVRRKVKAAADRVLANALLEQEISRSITEAALLERYNKTVAGRPGPDE
                     ARVRVIMMPSEEEAASIIAELRGGADFAAVAKRSSKDTTAPAGGDAGFVTRDGLTAEV
                     GAVVFSLPAGQVTPYPVRSAGAWFVLKVEERRRQTARAFSTVREDLRQAILREGVGDV
                     VKAAMADVTVREYDITGKETEASSADAGARAPR"
     gene            4519..5220
                     /locus_tag="VGC82_15745"
     CDS             4519..5220
                     /locus_tag="VGC82_15745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY0424773.1"
                     /translation="MRLTRTFLLCGLIACASLGAAGHARAQTTLLANLANGSFTYGDT
                     SFTISGCNYSGGACTTDNAKIIPVFNGRGGTEIEIVQQNGSAIFTNAVNAANTTLSFT
                     LKVTPQAGSRGLSAIQDILAGSAGTASEGNTRVTSLLDSFSVTATPASISSNLNATST
                     GTSFALTSSPVTFNVSLAVTSRGTTGVDTLQLNNVKLWFTPAPEPASIAMFATGLIAL
                     SAARRRSRQTPSPTR"
BASE COUNT          985 a         1686 c         1676 g          974 t
ORIGIN      
        1 ggtgcaaacg caaacaggtt cgggttacac tttgtcgcat cggggacttc ctctgctgcg
       61 cgataaggct ttgtcgcaga agaactttcg ctcattcaga gccctgatgc acctgactct
      121 tgtgagagat gcgggctagg aaggcagcat cgttcgcgtt cgcagcggtg ggatgttccg
      181 cagcgttttc acgccgactg agtaagtccg agccagttgc cgcctggcgt ctatgtgtca
      241 tggtcgtgct tggcgcggcc atgatagctt ttttgcagcc gcgagagaaa cccggtttca
      301 gtcaccgcga aaccgctcta atcctcgaac agccctaccg gcgcaccggc cgccggcgcg
      361 gcggcccgga acaatcctcc cagcgccgga tggtccgcca gaacctgggc ggattttccc
      421 tcccgcagcg acgtcacgta caagctgccg tcggcaaagc aaggcatggt tgggcccggc
      481 accggaaacg cccacttgcg cagcagcttt cccgcagggc tgaagcagtt gaggcacccg
      541 gccgacggac cggcggacca gtagtttcca tccatatcgg tcgcggcgcc atcgggacgg
      601 ccgtcgtcgt tggtcaatgt cgccaggatg cgatggttgc cgcgatcgcc ggtcgtcgga
      661 tcgaaatccc aaacctcgat gatgccggcg gtcgagtcgg agtgatacat ggtgcggcca
      721 tccggcgacc atgccaggcc gttagacacc gcgtatccgt ccgcctgcct ggtgatccgc
      781 ccgtcggaag tgacgcggta cagcccggcg atcttctggc gcggtgagtt ctcgtccatg
      841 ctgccgaccc agaacgcacc gtccggcccg accttgccgt cattcaatcg attggtcgcc
      901 ggctcgtcga ttttcggcgt caaatcggtc agcgcgccgg atgcgggatc gaacagcacg
      961 acatgatgcc gctgtgcgac aaccaggcgg cccgaccggc acaggccaaa gctgccgacg
     1021 acttcgggaa aatcccacga ttgccgcgct ccggtatcga cggtcagcgc gttgattcgc
     1081 ttgccgttga tatcgcagaa gagtaaccgc cttgttgccg cgtcccatac cggagactcg
     1141 gcgaccccgc agcgcgagtc ccagaccagt tcgaaatcgc tcatgccgtt gcgtccttta
     1201 cctcgtcagg tccgtctgcc gttccgaaac accggactac cctttgcaac gcgcgtcaaa
     1261 ctcgcccggt tgacttgaac cgcgcagccg aaaggaaagg ctcgaaagtg cagtctggga
     1321 gacggatgga taaaatcgag gctgtgtttg gcgatcatgc gcaactcgcg caccaactgc
     1381 tgtcggacct tggtcccgac ggcgacgatg gatcgcatga ccgttcgcac ctgctgcggg
     1441 tctggcgaaa cgcggcagac atcgcggcgc gggaatccgg ctgcgattgg gcggtgctga
     1501 cggctgcggt tatcctgcat gactgtgtag cggtggaaaa gaactccccg caacgctcac
     1561 aagcatcccg gctggccgcg gctcgggcgc gaaccatcct ggcgggactg gcctggagtc
     1621 cggccatcat tgaccgggtc gcccacgcga tcgaggccca cagcttctcc gctggcatcg
     1681 cgccggagac tatggaagcg cgggtcgtcc aggatgccga ccggctggat gccatcggcg
     1741 cgatcgggat cgcccgctgc ttctatgtcg ccggccggat gggttcgggt ttatacgatc
     1801 ccgacgaccc cgccgcggcg aaccggcctc tggacgaccg gaactttgcg ctggaccatt
     1861 ttggcgcgaa gctgttccac gtcgccggaa acttccagac caaagccggt caagccttgg
     1921 cagatgcgcg gacccggaca atgcatgact tcgtgcgggc gatgctgaac gagatcggaa
     1981 aggccagcga atgatacgag cagcgatcgt aggactgggc tggtggggcc agaatctcgt
     2041 caacagcgtg cgcggcagca gtgccatccg tttcaccacg gcgcataccc gcacgcgaac
     2101 cacagtcgcc ggcttctgct ccgaggccgg cctgcggtgg gtggattccc ttgacgagat
     2161 tctgaacgac aacgagatca atgccgtggt gttcgcgaca ccgcacacgc aacatgtcga
     2221 tcaggtggtt cgcgcggcag gcgccggcaa gcatgtcttt gtggagaaac ccttcggcct
     2281 gtcggtcgag gacgccatcc gagcgaggga cgcggcggac agggcgggga tcgtgctggc
     2341 ggtgggcttc aaccgccggt tccatccctc gatgggtatg ctccgaaccg ccgtcaccag
     2401 tggccggctc ggtaccttgg tcaccatcag cgcggagcaa acggcgctgc atggcatcac
     2461 catgatgccg gaggcttggc gcgcccagcc gaccgaggcc ccagccggcg cgatgacagc
     2521 catcggtgtt catctggttg acggcatgat cgacttgttc ggcccgatcc gtcaggttta
     2581 tgcccaggtg gcccgacgcg ctgcaccgct tgccgatgat accaccgacg tcatgctgcg
     2641 gtttgccaat ggtgcctccg ggcatatctt ttgctcgacc gctgccacgc cgcattaccg
     2701 aatggcggtc tatggcacga agggttttgc cgaggtgctg ggacatccga tgacgacgtt
     2761 ccggctgata ccggccggcg acggccaccc ggcggcaggc acgccggaag tgactgagac
     2821 cacaggtttc aacatgctga acgcggaact ggaagccttc gctgccagca tcgtcgcccg
     2881 ccagccattc ccgacgccac tgcgtgagat catgcacggc gtggcggtgt tcgaggcgat
     2941 cgccgcgtcg gcctcgacag gtcagccggt cgaggttcct ccggtctgat catttctcac
     3001 tctgatcatt ccaatcgcat cgcgagtggc cggcgtgcta tgggctcgcg gtcattgcgt
     3061 gcgggtgttc atgtcaggtc gaaaccgtaa ctgccttggg atactgctgt tgctggcgct
     3121 agccgcccgt ccggcctggg cgcagcccat ttcggcgggg aagccctcgc cgtttgcacc
     3181 tggcgaccta aaacgaccaa ttttcgacac cgccaagccg gtttatgaca ccgccaacca
     3241 tcaggaacgc agtgccagtt cggtggtggc cgaagtcgat gggcgggcga tcaccctcgg
     3301 cgaagtcggc gacgccatca aggagctgcc ggccagcgtg cagcacctgc cgttcgccga
     3361 cctgtttccc gcggtgttga cgcagcttgt ccgccagcag gcgctggtga tccggtcgca
     3421 gcaccaggca ctcgatgagg agccgacggt gcggcgaaag gtcaaggcgg ctgccgaccg
     3481 cgtgctggcg aacgcgctgc tggagcagga aatttcacgc tccatcaccg aggcggcatt
     3541 gctggaacgt tacaacaaga ccgtcgccgg caggcctggc cccgacgaag cgcgggtacg
     3601 cgtgatcatg atgccatcgg aagaggaggc ggcgagcatc atcgccgaac tccgtggtgg
     3661 agcggatttc gccgccgtcg ccaaacgttc cagcaaagat acgactgctc cagccggcgg
     3721 ggatgccggc ttcgtcacgc gggacggact gacggccgag gttggtgccg tggtcttttc
     3781 tctcccggcc ggacaggtca cgccttatcc ggtcagaagc gccggtgcat ggttcgtact
     3841 gaaggtcgag gagcggcggc gccagaccgc gcgggcattc agtaccgtgc gcgaagatct
     3901 gcggcaggcg atattacggg aaggcgtcgg tgacgtcgtc aaggcggcga tggcggacgt
     3961 cacggtccgg gaatacgata taaccggcaa ggaaacggag gcatcctccg cggatgcggg
     4021 cgcccgcgcg ccgcgctaga gcttgatcac gctgggcgga acgtccgtcg tccgacggac
     4081 gcgctgttcg ggcgatgcgt gtgaatcacg cgatcggaca aagggataaa tcattttcag
     4141 cctgcacagt caggctgaaa atgtactagc aggcataatt gctctgggtg gactgtctga
     4201 accgatcacc aaccggcatg gagatcgata ctctgccggc accacctcgt catgcggacg
     4261 aaggtcggca tccacgcctt ccgtgcgtat caccaaagcc gtggatgtgg cccttcggcc
     4321 accatgacgt cgagaggctg atgcggcctg catagtcacg cagaaacgcc acgcatccga
     4381 agattggcgg cgtctcattg atatccctgg catccgagga ccgaacgatt cctcgtttac
     4441 atttcggatt ggtccgagta tgttatggat gcgacagaag acggtttagc cgcgcctctt
     4501 cttgtcgcag gagttttcat gaggcttacc cggacgttcc tgctctgtgg cctgatcgct
     4561 tgcgccagtc tcggcgcggc gggacacgcg cgcgcccaga ccaccctttt ggccaatctt
     4621 gcgaacggca gcttcaccta cggggacacg tcgttcacaa tcagtggttg caactattcc
     4681 gggggcgctt gcacgacgga caacgcgaag atcatcccgg tgttcaacgg ccgcggcggg
     4741 accgagatcg agatcgtgca gcagaacgga agtgccattt ttactaacgc cgtaaacgcg
     4801 gcgaacacga ccctcagctt cacgctcaag gtcacgcccc aggccggcag tcgcggcctg
     4861 tccgcgattc aggatatact cgccggttcg gctggcaccg cctccgaggg aaatacccgg
     4921 gtcacgtccc tcctggacag tttcagcgtc acggccacgc cggcttcgat cagttcgaac
     4981 ctcaatgcga catcgaccgg taccagcttt gcgcttacct caagtccggt cacgttcaat
     5041 gtcagcctcg ccgtgaccag tcgaggcacg accggggtag acacgcttca gttgaacaac
     5101 gtcaagctgt ggttcacgcc ggcgcctgaa cccgcctcca tcgccatgtt cgccactgga
     5161 ctgatcgcac tgtccgccgc gcggcgccgg tccaggcaga cgccctcgcc aacgcggtag
     5221 ccgcacaact gtcacttgcg ccggcgacct tgtgccgaga accagtgacc accgaatccg
     5281 agagcggatc agacctcgga ataaccggac ccgcgtgtgg t
//
LOCUS       DASWQN010000923         3092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1671804, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000923 DASWQN010000000
VERSION     DASWQN010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671804"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1113)
                     /locus_tag="VGC82_15750"
     CDS             complement(40..1113)
                     /locus_tag="VGC82_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008491944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0424774.1"
                     /translation="MSDLIVIGGGLVGGAIAWGAQAKGADVILLDEGDIAFRAARGNF
                     GLVWVQSKGAGMPPYAHWTRQSAALWPALAARLRETTGADTALHQPGGLAFCLSDQEY
                     EDRCSLLRRMHNESGDIGTTMLSREEVRAMVPKLGDEVTGASFCPIDGHASPLYLLRA
                     LHQDLDYRPNRKVTHIDAAPNNFTVYAGNETFTTPRVVIAAGLGSRDLAPMVGLHVPV
                     SPLQGQIIVPERLRPLLDYPTHILRQTDEGSVMLGDSQEDVGFDTSTKIAVLKKIAER
                     NTRIFPALKDASIIRMWAALRVMSPDGFPIYEQSIRYPGAFAATCHSGVTLAGAHALA
                     LAPAILDGVLPDALSAFTAGRFQ"
     gene            complement(1110..2084)
                     /locus_tag="VGC82_15755"
     CDS             complement(1110..2084)
                     /locus_tag="VGC82_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0424775.1"
                     /translation="MATWLVRRLIQACFVVLAMTVIVFVGVHVIGNPVDILISPEADQ
                     AERARIIAEMGLDQPLWRQYVVFLNGALHGDLGRSFVFNEPALRLIAQRMPATLELAV
                     TAVLMSILVGVPLGLYAGMRPDGFLGRTIMAGSILGFSLPTFWVGLMLIMVFAVQLGW
                     LPSTGRGETAMLLGIRWSFLTWDGLRHMFMPALNLSLFNISLVLRLTRAGVREILPLD
                     YIKFARAKGLSPSRIVLVHVLKNIMIPVVTVVGLELGSTIAFAVVTESVFAWPGMGKL
                     IIDSINVLDRPVIVSYLMMIVLLFIVINLLVDILYTLLDPRIRLETKG"
     gene            complement(2085..>3092)
                     /locus_tag="VGC82_15760"
     CDS             complement(2085..>3092)
                     /locus_tag="VGC82_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424776.1"
                     /translation="ISYRSGDRIEMERFDGYWGAKPHWQHVNYRIISNDAARTASLLA
                     GDVDIIEFVPPTDLPKLRADPRVTLEEVVGNRAIYLWLDQSRTGPTPFVTGPNGETLT
                     SNPLKDRRVRLALSLAVNRQGIVDRVMEGAAVPTGQYLSPGSYSYVPDLKAPAYDPAK
                     AKALLTEAGFPNGLSITLNGPNDRYVNDAKIIQAVGQMWQRIGVKTTVEPLPWNSFVT
                     RAGKQEFSAFLLGWGTATAEGTNPLRAQIATWNPERGMGTANRGRYSNPEVDALIDQA
                     LATMDDAAREKIIQQATRQAMDDVPVIMLHLQKNIWAVRKGLTYEPRADEETRAMAVR
                     P"
BASE COUNT          579 a          978 c          991 g          544 t
ORIGIN      
        1 agcagccgga cgttttcgcg cactgaggct ggtcgcgtcc tactggaagc gtccagccgt
       61 aaaagccgac agcgcatccg gcagcacccc gtccagaatg gccggcgcca gtgccagcgc
      121 gtgggcgccg gcgagcgtca ccccgctgtg gcacgtcgcg gcgaaggccc ctggataacg
      181 gatcgattgc tcgtagatcg ggaagccatc gggcgacatc acgcgcagcg cggcccacat
      241 gcggatgatc gaggcgtcct tcagcgccgg gaagatccgc gtgttgcgtt cggcgatttt
      301 cttcagcacc gctatcttcg tggaggtatc gaagcccaca tcctcctggc tgtcaccgag
      361 catgacgctg ccttcatcgg tttggcggag gatatgggtg gggtagtcga gcagcggacg
      421 cagccgttcg gggacgatga tctggccctg caacggcgaa accggcacat gcaggcctac
      481 catcggagca aggtcccggc tgccgaggcc ggccgcgatg acgacgcgcg gcgtggtaaa
      541 ggtttcgttc ccggcgtaaa ccgtgaaatt gttcggcgcg gcgtcgatat gggtgacctt
      601 ccggttcggc cggtagtcca ggtcctggtg cagcgcgcgc agcaggtaca aggggctggc
      661 atggccgtcg atcgggcaga agctggcgcc ggtgacctca tcgccgagtt tcggcaccat
      721 ggcgcggacc tcttcgcgcg acagcatggt cgtgccgatg tcgccgcttt cgttgtgcat
      781 gcgacgaagc agcgagcaac ggtcttcgta ttcctggtcg gagaggcaga aggccaatcc
      841 gccgggctgg tgcagcgccg tatcggcgcc ggttgtttcg cgcagccggg cagcaagcgc
      901 cggccacaac gcagccgatt gccgggtcca gtgggcgtag ggcggcatcc ccgcaccttt
      961 gctttggacc cagacaaggc cgaaattgcc gcgggcggcg cggaaggcga tatcgccctc
     1021 gtcgagcagg atgacgtcgg cgcctttcgc ttgtgcgccc caggcgatgg ccccgccgac
     1081 caggccgccg ccgatgacga tcaggtcgct caacccttcg tctccagccg gatgcgcgga
     1141 tcgagcaggg tatagagaat atccaccagc agattgatga cgatgaacag cagcacgatc
     1201 atcatcagat acgaaacgat gaccgggcgg tccaggacgt tgatgctgtc gatgatcagc
     1261 tttcccatgc cgggccaggc aaacacgctt tccgtcacaa ccgcgaaggc aatcgtcgaa
     1321 ccgagctcca gcccgacaac cgttactacg gggatcatga tatttttcaa cacatgcacc
     1381 agcacaatgc ggctcggtga cagccctttc gcgcgggcga acttgatgta gtccagcggc
     1441 aggatctccc gcaccccagc gcgggtcagg cgcagcacca gggagatgtt gaacagcgaa
     1501 aggttcagcg ccggcatgaa catgtggcgc aatccgtccc aggtcaggaa cgaccatcgg
     1561 atgcccagaa gcattgcggt ctcgccccgt ccggtagagg gcagccaacc gagctgcacg
     1621 gcaaacacca tgatcagcat caggccgacc cagaaagtcg gcagggaaaa tcccaagatg
     1681 ctcccggcca tgatggtgcg gccgagaaat ccgtccggcc gcataccggc gtacagcccc
     1741 agcggcacgc cgaccaggat cgacatcagc accgccgtca ccgccagctc cagcgtcgcc
     1801 ggcatacgct gggcgatcag cctcagggcc ggctcgttga agacgaaact gcgtcccagg
     1861 tcgccatgca gggcgccgtt caggaacacc acgtattgcc gccacagcgg ctgatccagt
     1921 cccatttcgg cgatgatcct ggcgcgttcg gcctggtcgg cctcgggaga gatcaggata
     1981 tcgacgggat tgccgatgac gtgcacaccg acgaaaacga tgacggtcat ggccagcacg
     2041 acgaagcagg cctggattaa acgccggacc agccacgtcg ccatctatgg tcgcaccgcc
     2101 atcgcccggg tttcctcatc ggctcggggt tcgtaggtca ggcctttgcg caccgcccag
     2161 atattcttct gcagatgcaa catgatgacc gggacatcgt ccattgcctg gcgcgtcgcc
     2221 tgctggatga tcttctcgcg cgccgcgtcg tccatcgtcg ccagtgcctg gtcgatcagt
     2281 gcatcgacct cgggattgga gtagcggccg cgattcgcgg ttcccatccc acgctccggg
     2341 ttccaggtgg cgatctgcgc tcgcagcgga tttgtgccct cggcggtggc ggtgccccag
     2401 cccagcagga aggcggagaa ttcctgtttg ccggcgcggg tgacgaaact gttccatggc
     2461 agcggctcga ccgtcgtctt cacgccgatg cgctgccaca tctggccaac ggcctggatg
     2521 atcttggcat cgttgacata gcggtcgttc gggccgttca gcgtgatgct caggccgttg
     2581 ggaaagccgg cttcagtcag caaagccttc gcctttgcag ggtcgtacgc cggcgccttc
     2641 agatcaggca cgtagctata cgatcctggc gagagatact gtcctgtcgg gacggcagca
     2701 ccttccatga cccgatcgac gatgccctgg cggttgaccg caagcgaaag cgccagccgc
     2761 acgcgccggt ccttgagcgg gttggacgtc aatgtctcgc cgttggggcc agtcacgaaa
     2821 ggcgttggac cggtgcgcga ctgatccagc cacaggtaga tcgctcggtt tccgaccact
     2881 tcttccaagg tcacgcgagg atcggcacgc agtttcggca gatcggtcgg cggcacgaac
     2941 tcgatgatgt ccacgtcgcc cgccagcaaa gacgcagtac gcgcggcatc gttgctgatg
     3001 atacgatagt tgacgtgctg ccagtgcggt ttggcccccc agtagccgtc gaagcgctcc
     3061 atttcgatgc ggtcgcccga gcggtaggag at
//
LOCUS       DASWQN010000924         2574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_746787, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000924 DASWQN010000000
VERSION     DASWQN010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746787"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..537)
                     /locus_tag="VGC82_15765"
     CDS             complement(121..537)
                     /locus_tag="VGC82_15765"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY0424777.1"
                     /translation="MRPKAGQDGFTLIEMIVVIVIMAMVSGLVLVKQRWHSPGLNTEA
                     SLRALVNGLRLARSRAIAQDREVSVVTVANGFAVDGRSTWELPPEQALSTSKVVFMPD
                     GGSTGGTILLASGQRRIAVDVNWLTGRVRSVEMNKP"
     gene            complement(539..859)
                     /locus_tag="VGC82_15770"
     CDS             complement(539..859)
                     /locus_tag="VGC82_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424778.1"
                     /translation="MLMLLPLAFRGISPVSALERGPVCREATVVDEMTREVQVRNYYG
                     IVDPRLVTEQPTTDPGLVRCQVCVQSAPYDTLRFGDRPIAQCLRHDFEVRILTGGFVV
                     RDLR"
     gene            complement(945..1745)
                     /locus_tag="VGC82_15775"
     CDS             complement(945..1745)
                     /locus_tag="VGC82_15775"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY0424779.1"
                     /translation="MNDLRDAAWLWPLLVSPFVGSFLGVLIVRLPARRPVGMSRSICP
                     QCGTRLEAADLIPLVSFLVLRGRCRHCRAPIGIFHPAIEIAAVAVALWAVFGDSDLWH
                     DPGHDLARIWIGCALGWTLLTLAWIDWTNLVLPDVLTLPLLLAGLAVTVAFDADELTD
                     HCLAAIVAYLSFAGLAFAYRRLRGRDGLGGGDSKLIAAAGAWCGLAALPLVVLGSALL
                     GLLAALGLALAGRTMTSTTRIPFGPCIALAFWLVWLHGTMADDLIGWR"
     gene            2256..>2574
                     /locus_tag="VGC82_15780"
     CDS             2256..>2574
                     /locus_tag="VGC82_15780"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HEY0424780.1"
                     /translation="METSVTDRQLDALGEILLRQGHCDQRTLERARRVAIESDQRLDS
                     VLIQLGLVAERGLAEAYANLLSIPLATADRYPTVDPVLPDCLSAAFLRHARAMPVAAD
                     RQTV"
BASE COUNT          486 a          859 c          815 g          414 t
ORIGIN      
        1 cccgtgggtt ggtgtgcctg cgggttgatg tggcggttgc gcgccaaatc gataatgtgg
       61 cgccatccaa ggccgatagc cagatcgccg atgccgcctt gcggtgtgcg aagtaacgac
      121 ctatggtttg ttcatttcaa cggaacgcac gcgcccggtc agccaattca catctaccgc
      181 tattcgccgc tggccggaag caagcaggat ggtgccgccg gtcgatccgc cgtccggcat
      241 gaacaccacc ttggatgtgc tgagcgcctg ttccggcggc agctcccagg tggatcggcc
      301 atcgacggca aagccgttcg ccacggtgac gaccgagacc tcacggtcct gggcgatcgc
      361 gcgcgaccgg gccagccgca ggccattgac gagggcccgc aggctcgctt cggtgttcag
      421 cccggggctg tgccaacgct gcttgacgag tacaagtccc gagaccatgg ccatgatgac
      481 gatcaccacg atcatctcga tcagggtgaa accatcctgg ccggcctttg ggcgcatgtt
      541 accgcaaatc tcgcacgaca aagccgccgg tcaggatgcg tacctcgaaa tcgtggcgca
      601 ggcattgcgc gatcgggcgg tcaccgaacc gcaaagtgtc gtagggtgcc gattgcacgc
      661 aaacctggca gcgcaccagg ccgggatcgg tggtcggttg ttcggtcacc aaccggggat
      721 cgacgatgcc ataatagttc cgaacctgga cttcgcgggt catttcgtcc accaccgtcg
      781 cctcgcggca gaccgggcca cgttcaagcg cggacaccgg gcttatgccg cggaacgcga
      841 gcggcaggag catcagcatg gccgcaaccc tctgtcgcat cattgcctcc cggtggtccg
      901 atcctgccgc ttgttcccga gcgtcacgtg aatcggccca caaatcaacg ccacccgatc
      961 aggtcgtctg ccattgtccc atgcagccat acaagccaga aggccaaggc gatgcacggg
     1021 ccgaacggta tccgtgtcgt agatgtcatg gttcggcccg cgagtgccag tcccaacgcc
     1081 gcgagcaggc ccagcagcgc gctgcccaac accaccagcg gcagagcggc caggccgcac
     1141 cacgcaccgg ccgcggcgat aagcttgctg tcaccgccgc ccagcccgtc gcggccacgc
     1201 aaccggcggt aagcgaaggc caagccggcg aacgacaggt aggccacgat ggccgcgagg
     1261 cagtgatcgg tcagttcatc cgcgtcgaat gccacggtca ccgccaggcc ggccagcaac
     1321 aatggcaagg tcagcacatc cggcagcacc aggttggtcc agtcgatcca tgccagcgtc
     1381 agcaaggtcc atcccagggc gcaaccgatc cagatccgcg caaggtcgtg cccagggtcg
     1441 tgccaaaggt cgctgtcgcc gaacaccgcc caaagcgcga ccgcgaccgc ggcaatctct
     1501 atcgcgggat ggaatatacc aattggcgcg cggcagtgac ggcatcggcc gcgcagcacc
     1561 aggaagctga cgagcgggat aagatcggcg gcctccaatc gtgtgccgca ctgtggacaa
     1621 atcgaccgcg acatgccgac cggcctccgc gcggggagac ggacgatcag cacgcccagg
     1681 aaactgccga cgaacggcga caccaacagc ggccaaagcc aagccgcatc gcgcaggtcg
     1741 ttcatctgga gtctgctacg aaacagccgc ttcggccgcg aactttgatg ttgtcatggc
     1801 cgtaggcagt ctccgtccgc aagtaaacgc gtcgcgtcga gcgtgccctg gtcggtcggt
     1861 tctgctgggt tggccggacc gtgcgccagt cggaacagtt aatcatggcc ggaccggcgg
     1921 cgcagaaggc cgcgcgacgc ggcggccaac ccggcgcacg ctgggcgaag cacgtcatga
     1981 cgttgctgga ctgtgccacc ccgctcgaag cggttatctg tcatcaaacc gttgcatgcc
     2041 tggcccttac atgcctggac agtggttcct gttcggacca gcatcgctcc tcgcgacctg
     2101 gatcggacag tcgccgaccg tcggtggccc gaatttggca cagccacccg atcctgtcat
     2161 gcccggctgg ccggcgaagc ccggatcgct gacctttcat ttgcctgcga tccgatggac
     2221 ggcttatgcg tacggcgggg taggaaaagg cgtggatgga aaccagcgtc accgatcgac
     2281 agctcgacgc cctcggcgag atattgctgc ggcaaggcca ttgcgaccag cggacgctgg
     2341 aacgtgcgcg cagggtcgcg atcgagtccg accagcggct ggattccgtg ctcattcagc
     2401 ttggtctggt cgccgaacgg gggctggccg aggcttatgc caacctgctt tcgatccccc
     2461 tcgccacggc cgaccgctat ccgaccgtcg atccggtact gccggattgc ctgagcgccg
     2521 cattcctccg gcacgcgcgg gcgatgccgg tcgcggcgga ccgccagacc gtga
//
LOCUS       DASWQN010000925         3797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2596712, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000925 DASWQN010000000
VERSION     DASWQN010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2596712"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VGC82_15785"
     CDS             <1..430
                     /locus_tag="VGC82_15785"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424781.1"
                     /translation="PNVPLALSQGTFDGFVSTNESVATAKLWEAGVKYSYQDHQYVGQ
                     YMPMISDAFWTKLDPDLQKIMVDTWKANIGTYRANAAASQARARKLLEDSGVGFTDPS
                     AETIEKARKAMQADVAALVKDSKLSPEIVRLSEDAVNGSA"
     gene            433..1014
                     /locus_tag="VGC82_15790"
     CDS             433..1014
                     /locus_tag="VGC82_15790"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY0424782.1"
                     /translation="MAARPDTIWDTVERTLVGLLGALAMLVGLVQVLGRYLFPSMAIS
                     SAEEVIVYLVVWGVMIISSQLVRTDGHVRPDLVLRLVPPGGQRWLEAFNCLVALVFCG
                     GMMWYGWQIVDTSLMLEERSSSGLQFPMWIYYASLPAGGALMTMRYLIRLYRYVFHFD
                     PTTTTVGHHIPDHELPVSLTAATPPGLTPRLDR"
     gene            1018..2298
                     /locus_tag="VGC82_15795"
     CDS             1018..2298
                     /locus_tag="VGC82_15795"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY0424783.1"
                     /translation="MGPILFMVMFFGLLSAGMPIFLVLGACAAVLYVVSGQPLIGVAQ
                     VIIDHLNSSTLMSLPLFVMAAAFMRQGGVAKALVDVSAAWLGGIRGSLGLVTVVACTL
                     FAAICGSSVATALAMGTILLPAMIEKGYPRSFALGVIGASGTIGIVIPPSLALILYGI
                     VAEQSVPRLFLAGILPGLLQAGAFFAWVWYDARRRNFPKEPSRPIGERLVVTVRALPA
                     LAVPVIVLVGIYGGIVTVTEAAALAAVVALLISLVFYRGFRLSQTLSVIADALRSAGT
                     IMLIVATALAFGHWMTESGVPAKLVTWTLAHNLATWQFLLAINVLLLILGCFLEVVAA
                     LLLVIPILAPALIPLGVDPVHFSIIFTHNMEIALIHPPVGLNLYVLSTISDAPIGEVI
                     RGILPFLILLLIVLGIITYVPALTMWLPQVVYGH"
     gene            2288..3097
                     /locus_tag="VGC82_15800"
     CDS             2288..3097
                     /locus_tag="VGC82_15800"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEY0424784.1"
                     /translation="MAIEPGDWPPPLLDAKDHGAPSVFQPENLLREARRQKNLPRGEV
                     PAVCVLDPDGDIVRASKAAARARRNPFWACYHTDMYEVEHGGEKFGVVGCAVGASFAV
                     LVAEQLFASGCRFLISVTSSGRIADAGPMPYFILIDRALRDEGTSLHYIPASRVAEAD
                     PALSALVAAGLNGHQPPVRQGATWTTDAPFRETEAAIEAARSQGLLAVEMEAAALYAF
                     AQARARPVICFAHVTNQMAQSDGDFEKGEASGTRDALSVIVAAARAWRQRA"
     gene            3094..3684
                     /locus_tag="VGC82_15805"
     CDS             3094..3684
                     /locus_tag="VGC82_15805"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0424785.1"
                     /translation="MKPEPNAASDLLGLLTNPIWPTAVLWVLLLLSGAIAFSAWQREP
                     WQRNGRALGIWLLRLVMGVMWWQQSLWKIPPNYDGLVFWMKQIVAHAAIPLQARLVDQ
                     IVVPNITVFGPLVYAAEVLIGGCLILGVLTRVAASLGLLMALNLWLGLYSAPNEWPWT
                     YGYLIVIQALFVIDPPGRCLGIDRQLSMDRRRASGR"
BASE COUNT          582 a         1182 c         1235 g          798 t
ORIGIN      
        1 gccgaacgtg cccctggcgc tgtcgcaggg cacgttcgat ggcttcgttt cgaccaacga
       61 gagtgtcgcc accgcaaagt tgtgggaagc tggcgttaag tattcgtatc aggatcatca
      121 gtacgtcggg caatacatgc ccatgatcag cgacgcgttc tggaccaagc tagacccgga
      181 cctgcaaaaa atcatggtgg atacttggaa ggccaacata ggaacttacc gtgctaatgc
      241 cgccgcttcg caggcgcggg cgcgcaagct gctggaggac agcggcgtcg ggttcaccga
      301 cccgtcggcc gagacgatcg aaaaagcccg caaggcgatg caggccgatg ttgcggccct
      361 ggtgaaggat tcgaagctgt cgcctgaaat cgttcgcctg tccgaggatg cggtgaacgg
      421 gtcagcgtga tcatggctgc ccgtcccgat acgatctggg acaccgtcga acgcacgctg
      481 gtcggcctgc ttggcgcgct tgcgatgctc gtcggcctcg tgcaggtcct ggggcgctac
      541 ctcttccctt ccatggcgat ttcctcggcc gaagaagtca ttgtctacct ggtcgtttgg
      601 ggcgtgatga tcatctccag ccagttggtc cgcaccgacg gccatgtgcg gcccgacctc
      661 gtgctgcgcc tggtgccacc cggcggccag cgctggttgg aggctttcaa ctgcctcgtc
      721 gccttggtct tctgcggcgg catgatgtgg tacgggtggc agatcgtcga tacgtcgctg
      781 atgctggagg aacgcagttc cagcgggctg cagtttccga tgtggatcta ctacgcctcg
      841 ctgccggcgg gcggcgcgct gatgaccatg cgttatctta tccggctgta ccggtacgtg
      901 ttccatttcg acccgacgac gacgacggtt gggcaccaca ttcccgatca cgagttgcct
      961 gtcagcctga cagccgcgac gcccccgggc cttacgccac ggctggatcg ctgacccgtg
     1021 gggccaatcc tgttcatggt gatgttcttc ggactgctgt ccgcgggcat gccaatcttt
     1081 ctggtgctgg gcgcctgcgc cgcggtgctc tacgtcgtct cggggcagcc gctgatcggc
     1141 gtggcacagg tgatcatcga ccacctgaat tcttcgacgc tgatgtcgct gccgctattc
     1201 gtgatggccg ccgcgttcat gcgccaaggt ggggtcgcca aggccctggt ggatgtttcc
     1261 gccgcctggc tgggaggtat tcgcggctct ttgggcctgg tgaccgtcgt cgcctgcacc
     1321 ttgttcgccg cgatctgcgg gtcgtcggtg gcgacggcgc tggcgatggg caccatcctg
     1381 ctgccggcga tgatcgaaaa aggctatccg cgcagcttcg ccctgggcgt gatcggtgcc
     1441 tccggcacca tcggcatcgt cattccgccc agcctggcgt tgatcctgta tggcattgtg
     1501 gcggagcaat cggtgccgcg gctgttcctg gccggcatcc tgccaggcct gttgcaggcc
     1561 ggcgccttct tcgcctgggt ctggtacgac gcccgccgcc gcaacttccc aaaggagccg
     1621 tcacgtccga tcggcgaacg gctggtggtg acggtgcgcg ccctgccggc cctcgccgtc
     1681 ccggtgatcg tgctggtcgg gatctatggc ggtatcgtca ccgtgacgga agccgccgcg
     1741 cttgccgcgg tggtcgcgct gctgatcagc ctggtgttct accgcggctt tcgcctatcg
     1801 cagaccttgt cggtaattgc cgacgcgttg cgcagtgccg gcaccatcat gctgatcgtt
     1861 gccacggcct tggctttcgg ccactggatg accgagtccg gcgtgccggc gaaactggtc
     1921 acctggacgc tggcgcataa cctggcgacc tggcagttcc tgctggcgat caacgtgctg
     1981 ctgttgattc tgggctgttt tctggaagtc gtcgccgcgt tgcttctggt catcccgatc
     2041 ctggctccgg cgctgatccc gctgggagtc gatccggtgc atttttcgat tatcttcacc
     2101 cacaacatgg agatcgcgct gatccatcct ccggtcgggc tcaatctgta cgtgctgtcc
     2161 acgatttccg acgcgccgat tggcgaggtg atcaggggaa tactgccgtt cctgatcctg
     2221 ctgctgatcg tactggggat catcacctac gttccggcgc tgaccatgtg gctgcctcag
     2281 gtggtctatg gccattgaac caggggattg gccgccgcca ctgctggatg ccaaggacca
     2341 cggcgctcca tcggtctttc agccggaaaa cctgctgcgg gaggccaggc ggcagaaaaa
     2401 cctgccacga ggcgaggtgc cggcggtctg cgtattggac ccggacggcg atatcgtgcg
     2461 cgcaagcaag gccgcggcgc gagccaggcg gaacccgttc tgggcctgct accataccga
     2521 tatgtacgag gtggagcacg gcggcgagaa attcggcgtc gtcggttgcg cggtcggcgc
     2581 gtcttttgcc gtgctggtgg cggagcagct attcgcctcc gggtgccggt ttttgatcag
     2641 cgtgacctcc tccggtcgga tcgcggatgc cggcccaatg ccgtatttca tcctgatcga
     2701 ccgcgcgctg cgcgatgagg ggacaagttt gcattatatt ccggcttcga gggttgccga
     2761 ggcggatcca gcgctgagtg cattggtcgc cgccggtctg aacggccacc agccgcccgt
     2821 gcgccagggc gccacctgga ccaccgacgc gccgtttcgc gaaaccgaag ccgcgatcga
     2881 agccgcccgg tcacaaggat tgctggccgt ggagatggaa gccgccgcgc tttatgcgtt
     2941 tgcgcaggcc cgggcgcgac cggtcatctg tttcgcgcat gtgaccaacc agatggcgca
     3001 aagcgacggc gacttcgaaa agggcgaggc cagcggcacc cgcgatgccc tcagcgtgat
     3061 cgtggcagcg gcgcgcgcgt ggagacaacg agcatgaaac ctgaacccaa tgccgcaagc
     3121 gaccttctcg gtctgctgac caaccccatc tggcccacgg ctgtgctgtg ggttctgctt
     3181 ctgctcagcg gcgccattgc attttcggcc tggcaacgcg agccgtggca gcgaaatggc
     3241 cgggcgctcg gcatctggct gctgcggctg gtgatgggcg tcatgtggtg gcagcaatcg
     3301 ctgtggaaga ttccgccgaa ctacgacggc ctggtgttct ggatgaagca gatcgtggcc
     3361 catgccgcta ttccgctgca ggcccgactt gtcgatcaga tcgtggtgcc gaacatcacg
     3421 gtttttgggc cgctggtcta tgcggccgag gttctgatcg gcggttgcct gatacttggc
     3481 gtcctgaccc gggtggcggc ttcacttggg ctgttgatgg cgttgaattt gtggctggga
     3541 ctttactcgg cgccaaacga atggccctgg acctatggtt atctgatcgt catccaggcg
     3601 ctgttcgtga tcgatccgcc cggccgctgc ctgggcatcg accggcaatt gagcatggat
     3661 cgaagacgcg cttccggccg ctgattgccc cgtccaccac gccccgccac agcgccgcgt
     3721 ccaccaggca aagaactatt cccgcacatc ggcacgccga acgccgcctc gtacatcggt
     3781 ggtgtattgg gcgccat
//
LOCUS       DASWQN010000926         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1408801, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000926 DASWQN010000000
VERSION     DASWQN010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408801"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VGC82_15810"
     CDS             <1..189
                     /locus_tag="VGC82_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424786.1"
                     /translation="DAATVRGIAAILDPWPFETLYGGWWERVIPARAKMVMASSVEQY
                     VRAVTGSPERSGSTGSPI"
     gene            complement(552..1016)
                     /gene="pal"
                     /locus_tag="VGC82_15815"
     CDS             complement(552..1016)
                     /gene="pal"
                     /locus_tag="VGC82_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY0424787.1"
                     /translation="MNIKILGALSAAALAAACTTPAAPPAAPTPVASGIVPGSQEDLV
                     ANVGDRVFYDYNMSTLRSDAKGTLDKQATWLAKYGSVNVQIAGNCDERGTEEYNIALG
                     NRRATAASSYLKAQGVAAARMSTISYGKDRPTAQGSNEQAWAQNRNAVTSVR"
     gene            complement(1288..2682)
                     /gene="tolB"
                     /locus_tag="VGC82_15820"
     CDS             complement(1288..2682)
                     /gene="tolB"
                     /locus_tag="VGC82_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010501075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEY0424788.1"
                     /translation="MIDTLTSSVGRRALIAGAAGLLAAPIPVSAQPAPPPASGGSGRS
                     AVIDVDKARTEPIPIAIPDLGGPDGASAQLGHDMAGVISADLARSGLFRLIAQTAFIQ
                     AGAPTAAAPNFQNWKPTGAQALVTGSVQILGGGQVRVEFRLWDILPQTQIQGTAYTTT
                     QGNWRRIAHIMADVIYERLLGEKGYFDTRIVYISATGPRDRRTKRLAIMDQDSENNRL
                     LTDGSWLVLTPRFHPSRDEIAFMSYANNRPRVYLFNLTSGRQSALGDFAGMTFAPRFS
                     PDGSAVVMAVANGGGSDIVAMDLNSRSPRRLTDSGSIDVSPCFSPDGSRIAFASDRGG
                     DQQIYVMGSGGGGAKRISFGNGRYATPVWSPRGDLVAFTRYAGDTSNFAIGVMHPDGS
                     GERILSEGWLVEGPTFAPNGRVLMFWRETRSQDARGRGFSSRLVSIDITGFNERPVET
                     PTDASDPAWSPLAM"
     gene            complement(2774..>3337)
                     /locus_tag="VGC82_15825"
     CDS             complement(2774..>3337)
                     /locus_tag="VGC82_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424789.1"
                     /translation="TPPVPAPPKPEAPLPLPPPLVPPPPAPPSATSQPNATKNPAPNS
                     RSVENTLEKLREQMAQATPPKNRANPQAGGQPTGGNPSGNDTAALTADQRGAIGDHVR
                     ECWTKDAGALDIDKQRVLLTVTTDATGIARKAEIAGDDIGRLSDPRFRAFAERARRAI
                     MDPRCANLPLPNNVLGKVNILTFRFSP"
BASE COUNT          541 a         1029 c         1145 g          622 t
ORIGIN      
        1 gatgcggcga ctgtgcgggg gatcgccgcg attttggatc cctggccgtt cgaaacgctc
       61 tatggcggct ggtgggaacg ggtcataccg gcgcgagcca agatggtgat ggcgtcgtcc
      121 gtggagcaat acgtcagggc cgtcaccggt tctccggaac gctccgggag cacgggcagc
      181 cctatttgat gggcatgttg gaatacagca tgatcacgct gggtggaacg tccgaagcgt
      241 gtgaatcacg cggtaatcaa ggaggaagtc catttggtgc cggcacagtc aggctgaaat
      301 tgctctagtg tcccgaatct gaggtccgcc gtatccggcg aaccccagtc tcgcgccact
      361 ctctctttgc ttcggtggcc tgctgatccc ttgcgtccgc cgtatgatgc gacgaacttc
      421 ggagcaggac actagcgctc gaagcgatca tttcgaagca gatcaaacaa gaaaatggat
      481 cgtttccaac ccgtgtagtc aagctggaaa cgatcaagat gcgaggcgat taccttccgc
      541 ccgccacacc ctcagcgaac ggaagttacc gcgttgcggt tctgcgccca cgcctgctcg
      601 tttgaaccct gcgcggttgg acgatctttg ccatagctga tcgtgctcat gcgcgccgcg
      661 gcgacgccct gggccttcag gtaggaggag gccgcggtgg ctcggcggtt gccaagcgcg
      721 atgttgtatt cctcggtgcc gcgttcatcg cagttgccgg cgatctgcac gttcacgctg
      781 ccgtacttgg cgagccacgt ggcctgcttg tccaacgtgc cctttgcgtc actacgaagg
      841 gtggacatgt tgtagtcata gaagacgcgg tcgccgacat tggcgacgag atcttcctgg
      901 ctgccgggca cgatgccgga tgcgacgggc gttggcgcgg cggggggagc agcgggggtg
      961 gtgcatgccg ccgccagggc agcggcggac aaggcgccga ggatcttgat gttcatcgtt
     1021 ttggtccctg gatggggtgc agtccaatgc ccgagcgtgc ccgcgcagaa agaatcggta
     1081 atccgttccg tccgctgacc gaataacgtg ttccgccagt accggtcaag ttctcccggg
     1141 cgctttcgtc aacacatgca attgttgttg cctcgtcaac acggtgtcgc cggagcctgc
     1201 gcagcccgga ctgtttgcgc tccgtagcca gcctccaagc ccagtgcccg gcagcgcccc
     1261 agcgcccggc agcgccccgg ggcgctgcta cattgcgagc ggcgaccagg ctggatcgga
     1321 tgcgtccgtg ggagtctcga ccgggcgttc gttgaaaccg gtaatatcga tcgacaccag
     1381 gcgcgacgag aaaccgcgcc cgcgcgcgtc ctgggaacgg gtttcgcgcc agaacatcag
     1441 cacacggccg ttgggggcga aggtcggccc ttcgaccagc cagccctcgg acaggatacg
     1501 ctcgccggac ccatccgggt gcatcacgcc gatggcgaag ttggacgtat cgccggcata
     1561 gcgggtgaag gccaccaggt caccgcgcgg cgaccatacc ggagtcgcat aacgaccatt
     1621 gccgaagctg attcgtttcg caccgccgcc gcctgacccc atcacgtaga tctgctggtc
     1681 gccgccgcga tccgatgcga aggcgatccg cgatccgtcc ggactgaagc aagggctgac
     1741 atcgatcgat ccggaatcgg tgaggcggcg cggcgagcgg ctgttcaaat ccatcgcgac
     1801 gatatccgat ccgccgccgt tcgcgaccgc catgaccacg gcgctgccat cgggagagaa
     1861 ccgcggggcg aaggtcatgc ccgcaaagtc gccaagtgcg ctctgccggc cagaggtcag
     1921 gttgaacagg tacaccctcg ggcggttgtt ggcgtagctc atgaaggcaa tttcgtcgcg
     1981 gctgggatgg aagcgcggag tcaggaccag ccacgatcca tccgtcagca gacggttgtt
     2041 ctcgctgtcc tggtccatga tcgccagacg cttggtgcgc cggtcacgcg gaccggtagc
     2101 cgagatgtac acgatccggg tgtcgaaata gcccttctcg cccagcagcc gctcgtagat
     2161 cacgtcggcc atgatgtggg ctatgcgccg ccagttgccc tgggtggtgg tgtaggcggt
     2221 gccctggatc tgggtctgcg gcagaatgtc ccacagccgg aattccaccc ggacctgacc
     2281 gccgccgagg atctgcacgc tgccggtcac caaggcctgg gcgccggtcg gcttccagtt
     2341 ctggaagttc ggcgccgccg ccgtcggcgc accggcctgg atgaaggccg tctgggcgat
     2401 cagccggaac agtccgctgc gggcaaggtc ggcactgata actcccgcca tgtcgtgacc
     2461 aagctgggcc gaggcgccat cgggaccacc aagatcgggg atcgcgatcg ggatcggttc
     2521 ggtccgcgcc ttgtccacgt cgatgacggc ggaacggccg ctgccgcccg atgccggagg
     2581 cggcgcaggc tgtgcggaca ccggaatcgg tgcggcaagc aggccggcgg cgccggcgat
     2641 cagtgcgcgg cggccgacag atgaagttaa ggtatcgatc atgccgtttt cgtccacatt
     2701 gaggttcggg ctcttgacgt tcggggcagc gcggccgacc gcgctatcgc ttggttgggc
     2761 cggacgtgcc ctgtcatggg ctgaagcgga aggtcagtat gttgaccttg cccagcacat
     2821 tgttcggcag cggcaggttg gcacagcgcg ggtccatgat tgcccgcctc gcgcgttcgg
     2881 cgaacgcgcg gaatcgcgga tcgctcaggc ggccgatatc gtcgccggcg atctcggctt
     2941 tgcgtgcgat gccggtggca tcggtggtga cggtcagcag cacgcgctgc ttgtcgatat
     3001 cgagtgcccc ggcgtccttg gtccaacatt cgcgcacatg gtcgccgatc gcgccgcgct
     3061 ggtccgcggt cagcgccgcg gtgtcgttgc ctgacggatt gccgccggtc ggttgtccgc
     3121 cggcctgcgg gttcgcccgg tttttgggcg gcgtcgcctg ggccatctgc tcacggagtt
     3181 tctccagcgt gttctccacc gaacgactgt tcggcgctgg gttcttcgtc gcgttaggct
     3241 gcgacgtcgc gctgggcggt gccgggggcg ggggcacgag gggtggcggc agcggcagcg
     3301 gcgcttcggg ttttggcggt gcagggacgg gcggtgt
//
LOCUS       DASWQN010000927         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1012232, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000927 DASWQN010000000
VERSION     DASWQN010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012232"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VGC82_15830"
     CDS             complement(<1..189)
                     /locus_tag="VGC82_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424790.1"
                     /translation="MRFAAKLALFLSLTLVVTQVATGLAIYTLIRDTLVDEGKAHLIT
                     AADRFIRQLQEIEGQVADG"
     gene            complement(199..873)
                     /locus_tag="VGC82_15835"
     CDS             complement(199..873)
                     /locus_tag="VGC82_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424791.1"
                     /translation="MRKPWLGREWRLGVAAIAFMAMAVAETAPGRAAAITATVTDEAG
                     KPLPDAVVSVTATGDVRIIPSTQLAAAEIDQRDETFVPSVVVIQPGGSVTFRNSDRTR
                     HHIYSFSPIRQFEFVQKPDDLSPSVSFEVPGVAAIGCNIHDFMIAYVYVTDAPRAAVT
                     DSRGRVEITDLPAGRFTATVWHPRLRTKAAPPSQSVTLESRSTMLAITISVLPPRRAR
                     DPKSPY"
     gene            1147..>1529
                     /locus_tag="VGC82_15840"
     CDS             1147..>1529
                     /locus_tag="VGC82_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEY0424792.1"
                     /translation="MINKIVQTMADAMAGIKDGSVVLLGGFGSVGQPNALIDGLIEQG
                     ATDLTVACNNAGAGHVGLARLMELGRVRKIICSFPRSSDPVVFETLYRAGKIELEIVP
                     QGTLAERMRAAGAGVPAFFTATGVGT"
BASE COUNT          297 a          465 c          486 g          281 t
ORIGIN      
        1 cccgtccgcg acctggcctt cgatctcctg caattggcgg atgaaccggt ccgcggcggt
       61 gatcagatgc gccttaccct catcgaccag cgtgtccctg atcaaggtat agatcgccag
      121 gccggtggcc acctgggtga ccacaagggt caacgacagg aaaagcgcga gcttggcggc
      181 gaagcgcacg atgtcgctct agtacgggct tttggggtcg cgggcgcgcc gtggcggcag
      241 caccgagatg gtgatcgcga gcatcgtgct cctgctttcc agcgtcaccg actgggaggg
      301 cggagccgcc ttcgtccgca accggggatg ccagacggtg gcggtgaacc ttcccgccgg
      361 caggtcggtg atctccaccc tgccccggct gtcggtcacg gccgcgcggg gtgcgtcggt
      421 gacgtaaaca taggcgatca tgaagtcatg gatattgcag ccgatcgcag caacacccgg
      481 cacctcgaac gagaccgacg ggctgaggtc gtcgggtttc tgcacgaact cgaactgccg
      541 gatcggcgaa aacgagtaga tgtggtgacg ggtccggtca ctgttgcgaa aagttaccga
      601 tccaccaggc tggatcacga cgacggaggg gacaaacgtc tcatcgcgtt gatcgatctc
      661 ggcggccgcg agctgagtcg aagggatgat ccgaacatcg ccggtggcgg tgacggagac
      721 gacggcatcg ggcagcggtt tgccagcttc gtcggtcacg gtggcggtaa tcgccgcggc
      781 ccggcctgga gcggtttcgg ccacggccat ggccatgaac gcgatcgcgg cgacgcccag
      841 gcgccattcc cggccaagcc aaggttttcg caacgaatcg ttccgcggat caggttccat
      901 cgttctcatt ttcgccgagg cggaatagac tcgcatcctc accgtttcaa taatacaaat
      961 aacgcgaaaa atcgatatta aatcgtatat gtaactaaca cgttctcggg tgcccgtcca
     1021 taccaccggc ataatactac ttagggcatt gatcgtgaca tcgcgcatga cgcatgcggc
     1081 ggccttggca cgtccgcggc gcagcgctac accggacgac caaacgagct gcaggaaacg
     1141 ccgcgcatga tcaacaagat tgtccagacc atggccgacg ccatggccgg catcaaggat
     1201 ggttccgtcg ttcttcttgg cggcttcggt tccgttggcc agcccaatgc tttgatcgac
     1261 ggactgatcg aacaaggggc aacagacctg accgtcgcgt gcaacaacgc cggtgccgga
     1321 catgtcggcc ttgccaggct gatggagctg gggcgcgtcc ggaagatcat ttgctcgttc
     1381 ccgcgcagct ccgaccccgt ggtgttcgaa acgctgtacc gcgctggcaa gattgagctg
     1441 gagatcgtcc cgcaagggac tttagccgaa cgcatgcgtg ccgcgggtgc cggcgttccg
     1501 gcgttcttta ccgccaccgg cgtcggcac
//
LOCUS       DASWQN010000928         6243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1539456, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000928 DASWQN010000000
VERSION     DASWQN010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539456"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2252)
                     /gene="cofH"
                     /locus_tag="VGC82_15845"
     CDS             complement(<1..2252)
                     /gene="cofH"
                     /locus_tag="VGC82_15845"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:NF005609.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEY0424793.1"
                     /translation="MIDMTSLQSAPLPELMAKAAHMRDEIFGRNISYSRKVFIPLTKL
                     CRDVCHYCTFAERPRPGHGAYLSADEVLAIARAGQAAGCTEALFTLGDKPELRYRAAR
                     EALTELGHETTIGYLREMCALVLKETSLLPHVNPGVMTREEIASLREVSASQGIMLES
                     TSERLCQQGGVHYGSPDKLPSVRLETIRLAGELAVPFTTGILIGIGETRPERLDALAA
                     IRDLHLAHGHIQEVIVQNFRAKPDTKLANADEPDLLDLLWTIAVARLILPADVHVQAP
                     PNLSPGVYQKLIGAGIDDWGGVSPVTPDHVNPEAPWPELEALGLRTAEMDKILVPRLP
                     VYPAWAADPARWLHPKVATRVMQSADAEGWARDDDWAPGLTFAPKPRSVLLNKADPVL
                     DRIIDRAAAGRRLDEPEIIRLFGARDADYERVVTAADALRKSVSGDVVRYVVNRNINY
                     TNICYYRCKFCAFSKGKTHEALRGTPYDLEMGEIVRRSQEAWDRGATEVCLQGGIHPD
                     YTGDTYVNICKAIKAAIPDMHIHAFSPLEVTQGAATLGLSLTEFLARLKDAGLSTLPG
                     TAAEILDDEIRAIICPDKINTQQWVDVARAAHALGLRTTSTIMYGHVETPLNWARHLL
                     VLRDLQAETGGFTEFVPLPFVHMEAPMYLRGMARKGPTLREAILMHAVSRLVLNPLIT
                     NIQTSWVKMGPEGAAMCLNAGANDLGGTLMNESISRAAGTQHGQEFPPEAMERLIGSL
                     VRTPVQRNTL"
     gene            complement(2249..3199)
                     /locus_tag="VGC82_15850"
     CDS             complement(2249..3199)
                     /locus_tag="VGC82_15850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY0424794.1"
                     /translation="MDFGIHIGTRGCLTTRENIMAVASSAEALGYAIIGVADHLIVPV
                     HTDVRYPYTADGIWPGAPTGECFDAIATLTFLAGFTQQIKLLTSVLVVPYRPAVLTAK
                     LLTTADVLSGGRIIAGVGSGWMKEEFAALGTPPFEDRGAVTDEYIRAWRSLWTEDRPS
                     IDGKFVKFDNVVFKPKPVSKPPIWVGGESAPALRRTAQYGDAWYPVSNNAHILMNTPA
                     LLKAGIERLHRAAEKAERDPTTIDIAYVWFMPPRWTAKTNAEGQRQMFTGSADDMLQD
                     AAAFAAIGVKHLIVYTQQPTIEATLDVQQRFAEDVVRKSQ"
     gene            3331..4284
                     /locus_tag="VGC82_15855"
     CDS             3331..4284
                     /locus_tag="VGC82_15855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY0424795.1"
                     /translation="MQIGCSAPTSGPLIEPDSLLRIATEAEVLGFDYVTVSDHVMIPT
                     SIASRYPYSDSGEFPSGAAAPRLEQLTAATFIAAVTSKLRIVTSVMVVPHRPAVLTAK
                     ILATIDFLSKGRLTLGIGAGWCEEEFIAIGAPPFAERGAVTDEFMAVCKELWTSDQPR
                     FDGKYVQFKDVLFPPKPPQGSIPIWVGGESGPAMWRTARYGDAWYPIGTNPQFPMDTL
                     TRFKAGAEKLRGLTEKAGRDPASVALAYRVSSNPEAQPKGTVDGQRKLFTGGAADYVG
                     DIKALRDVGVTSFDFGLFGADAEASVANLRRFRDDVMAKVQ"
     gene            4287..5321
                     /locus_tag="VGC82_15860"
     CDS             4287..5321
                     /locus_tag="VGC82_15860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY0424796.1"
                     /translation="MQLGITVGMSGPHPKLDMEMILEAERLGYSQVWTGEAYSTDAVS
                     PAAWILARTTKIKAGTGIMQMPARTPACAAMTVLSLQALSGNRFLCGIGPSGPQVIEG
                     WHGVRFGKPMQRTKEYIAIIKQILAREKPLEFHGSEYSIPYDGPDASGLGKPLRSIAH
                     GDPNVPFYTASITPAGLRMAGEVADGNLPIFFSPDAPDVVTGPTLEGRRKVGKTMEGF
                     DTAPFVRAKMGPDVAACRDAIRPELALYIGGMGARSKNFYNDMVTKMGFEGEARVIQD
                     FYLDGKKNEAAAAVPDALIDAISLCGPAERIRDRLAAWKDVAKAGHVGTMVLKGSGVD
                     VLRVVAEAVL"
     gene            complement(5342..>6243)
                     /locus_tag="VGC82_15865"
     CDS             complement(5342..>6243)
                     /locus_tag="VGC82_15865"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0424797.1"
                     /translation="AHTPGGSSSGSAAAVGDFQVPLAFGTQTGGSHIRPASFNGIYGL
                     KPSWNLVSREGVRMSSMTLDTVGWYGRCVDDLILVGSAFRLPGDTTPVQVKGLRVGLC
                     QSPVWGQIEQGGEMALATAAERLAAAGARVFELVLPEPFNRLHDAHTAIVNAEGGASF
                     LPEYVNAHGILASDLKAKVENALQITPQRLLESYALADACRPVLDNMFGPEVDVILTP
                     SSCGEAPEGLHTTGDAVFNRMWTLLHGPNVGIPCCRGPKNLPVGVTLVGKRLTDGRLL
                     AIAKALAPVIDADPLAGLRALWG"
BASE COUNT         1101 a         1923 c         2072 g         1147 t
ORIGIN      
        1 tacagcgtgt ttcgctgcac cggcgtgcgg accagcgagc caatcaaccg ttccatcgct
       61 tccggcggga attcctgacc gtgctgggtg ccggcggcgc gggagatact ttcattcatc
      121 agcgtgccac caaggtcatt ggcaccggcg ttcaggcaca tcgcggcacc ttcgggtccc
      181 atcttcaccc aggaggtctg gatgttggtg atcaacgggt tcagcacgag gcgggagaca
      241 gcatgcatca ggatggcctc tcgcaacgtc ggacctttgc gtgccatgcc gcgcaggtac
      301 atcggcgctt ccatgtgcac gaagggcagg ggaacaaatt cggtgaaacc gccggtttcg
      361 gcttgcaggt cgcgcagcac gagcaaatgc cgagcccagt tgagcggagt ttcgacgtgt
      421 ccatacatga tggtggatgt ggtgcgcaga cccaacgcgt gcgcagcgcg agcaacatcg
      481 acccattgtt gcgtattgat cttgtccggg cagatgatcg cccggatttc gtcgtccagg
      541 atttcggctg cggtaccggg cagggtgctc agcccggcgt ccttcaggcg cgccaggaat
      601 tcagtcagcg acaagccgag tgtggcggca ccctgcgtca cctccagcgg cgagaaggca
      661 tggatgtgca tgtcggggat cgcggctttg atcgccttgc agatgttgac gtaggtgtcg
      721 ccggtatagt ccgggtgaat gccgccttgc aggcagactt cggttgcgcc tcggtcccag
      781 gcctcttgcg accgacggac gatttcgccc atttccaggt cgtagggggt tccgcgaagc
      841 gcctcgtgtg tcttaccttt ggagaaggcg cagaacttgc agcgataata gcagatgttc
      901 gtgtagttga tgttgcgatt gaccacgtaa cgaacaacat cgccgctgac cgatttgcgc
      961 agtgcgtcgg cggccgttac gacccgctcg tagtcggcgt cgcgagcacc gaacagacga
     1021 atgatctcgg gttcgtccag ccgcctcccc gccgcggcgc ggtcgatgat ccgatccagt
     1081 acaggatcgg ccttgttcag cagcacggac cgcggcttcg gcgcgaatgt cagtccaggt
     1141 gcccagtcat cgtcgcgcgc ccagccttcc gcatcggcgg attgcatgac gcgggtcgct
     1201 accttggggt gcagccagcg cgccggatcg gcggcccagg ccggatagac cggcagccga
     1261 ggcaccagga tcttgtccat ctccgccgtg cgcaggccca aagcttccaa ttccggccat
     1321 ggtgcttccg gattgacgtg atccggtgtg accggcgaca cgccgcccca atcgtcgatg
     1381 ccggcgccga tcagtttctg atagaccccc ggcgataggt tcggcggcgc ctggacatgc
     1441 acgtccgccg gcaggatcaa cctggccacg gcgatggtcc agagcaggtc gagcaaatcc
     1501 ggctcgtcgg cgttcgccag cttggtgtcc ggcttggcgc ggaagttctg cacgatcact
     1561 tcctggatgt ggccgtgggc caaatgcagg tcgcggatcg cggccagcgc gtccagtcgt
     1621 tcggggcgcg tttcgccaat gccgatcagg atgccggtcg tgaacggcac ggcgagttcg
     1681 ccggccagcc gaatcgtttc cagtcgcacc gaaggcaact tgtcgggtga cccgtaatgg
     1741 acaccgcctt gctggcacag gcgctcgctg gtggattcca gcatgatacc ctggctggcg
     1801 gaaacctctc gcaggctcgc gatttcctcc cgggtcatga ccccggggtt cacatgcggc
     1861 agcaggctgg tttccttcag caccagcgcg cacatttcgc gcaggtagcc gatcgtggtt
     1921 tcgtggccga gctcggtcaa cgcttcgcgc gcggcacggt agcgcagttc gggtttgtcg
     1981 ccgagggtga acaacgcctc ggtgcatccg gcggcctgac cggcgcgggc gatcgccagc
     2041 acctcgtcgg ccgacagata cgcgccatga ccgggacgag gacgttccgc gaaggtgcag
     2101 tagtgacaaa cgtcacggca cagcttcgtc agtgggatga agaccttacg ggagtacgag
     2161 atattgcgcc cgaaaatctc gtcgcgcatg tgcgccgctt tcgccatcaa ctcgggcagc
     2221 ggcgcggatt gcagcgaggt catgtcgatc attgtgattt ccgtactacg tcctcggcaa
     2281 agcgctgctg gacgtccagt gttgcctcga tggtgggctg ttgcgtgtag acgatcaagt
     2341 gcttgacgcc gatggcggcg aacgcggcgg cgtcctgcag catgtcgtcc gcgcttccgg
     2401 tgaacatttg ccgctggccc tcggcgttgg ttttcgcggt ccagcgcggc ggcatgaacc
     2461 agacataggc gatgtcgatg gtggttggat cgcgttcggc tttctcggcg gcacggtgca
     2521 agcgctcgat gccggccttc agcaacgccg gcgtgttcat caggatatgc gcgttgttcg
     2581 acaccgggta ccaggcatcg ccgtattggg cagtgcggcg cagggcaggg gcgctctcgc
     2641 cgcccaccca gatcggcggt ttcgacaccg gcttcggctt gaacaccaca ttgtcgaact
     2701 ttacgaattt tccgtcgatc gagggtcggt cctcggtcca cagagaccgc cacgcccgga
     2761 tgtactcgtc ggtgacggcg ccgcggtcct cgaacggtgg cgtaccgagg gcggcgaact
     2821 cctccttcat ccagccggag ccaacgccgg cgatgatccg tccgccggac aggacgtcgg
     2881 ccgtggtgag cagcttcgcc gtcagcacgg ccggccggta tgggaccacc agcaccgagg
     2941 tcagcagctt gatctgctgg gtgaagccgg ccaggaaggt cagcgtcgcg atggcgtcga
     3001 aacactcgcc ggtcggcgcg ccgggccaga tgccgtccgc ggtatagggg tagcgtacgt
     3061 ccgtatgcac cgggacgatc aggtggtcgg ccacgccgat aatggcgtag cccagagcct
     3121 cagcgctgga ggccaccgcc atgatgttct cccgcgtggt caggcagccg cgcgttccga
     3181 tgtggatgcc gaagtccatc gctggccgct cccccgttgc ctgtttgccc gatgcggtgt
     3241 gttatcggcg cccgggttgc cggtgccgat cgtcgtcggc ttatcgtgtg gtatcgggat
     3301 ccataacaca attcgatgcc ggaggtacct atgcagatcg gttgcagcgc cccaacaagt
     3361 ggtccgttga tcgagccgga cagcctgctt cgcatcgcaa cggaggcaga agttctaggc
     3421 ttcgattatg tcaccgtcag cgatcacgtc atgatcccga ccagcatcgc ctcgcgctat
     3481 ccctattcgg attcaggcga gtttccgtca ggcgcggcgg cgcccaggct ggagcagttg
     3541 actgcggcga cgtttatcgc ggcggtgacc tccaaactgc ggatcgtgac ttcggttatg
     3601 gtcgtgccgc accggccggc ggtgctgacg gcgaaaatcc tcgccaccat cgatttcttg
     3661 tccaagggcc ggctgacctt gggcatcggc gcgggctggt gcgaggaaga gttcatcgcc
     3721 atcggggcgc cgccattcgc cgaacgcggc gcggtgaccg acgaattcat ggcggtctgc
     3781 aaggagttgt ggacctcgga tcagccgcgg ttcgacggca aatatgtgca gttcaaggac
     3841 gtgctgttcc cgccgaagcc gccgcaggga tcgattccga tctgggtcgg cggcgaaagc
     3901 gggccggcga tgtggcgaac cgcccggtac ggcgatgcct ggtatccgat cggcaccaat
     3961 ccgcaattcc cgatggatac gctgacgcgc ttcaaggcgg gtgccgaaaa gctgcggggg
     4021 ttgacggaga aagccgggcg cgacccggcc tcggtcgcgc tggcgtatcg tgtctcgtcg
     4081 aatcccgagg cgcagccaaa gggaaccgtc gatggccaga ggaaattgtt caccggcggc
     4141 gcggccgact atgtcggcga catcaaggca ctgcgcgatg tcggcgtgac gtcgttcgac
     4201 ttcggattgt tcggcgccga tgccgaggct tcggtcgcca acctgcggcg gttccgcgac
     4261 gacgtcatgg cgaaggtcca atagaaatgc aacttggcat cacggttggc atgtcggggc
     4321 cgcatccgaa gctcgacatg gagatgatcc tggaggcgga gcgtctggga tactcgcagg
     4381 tttggaccgg cgaggcttat agcaccgatg cggtgtcgcc cgccgcatgg atcctggctc
     4441 ggaccaccaa gatcaaggcc ggaacgggga tcatgcaaat gccggcgcga accccggcct
     4501 gcgcggcgat gaccgtgctg tcgctgcaag cgctgtcggg aaaccggttc ctgtgcggga
     4561 tcggaccgtc cgggccgcag gtgatcgagg gctggcacgg cgtgaggttc ggcaaaccga
     4621 tgcagcggac caaggaatac atcgccatca tcaagcagat cctggcgcgg gagaagccgc
     4681 tggagttcca cggcagcgaa tactccatcc cttatgacgg gccggacgcc agcggcctgg
     4741 gcaaacccct gcgcagcatc gcccatggcg acccgaacgt gccgttctac acggcctcga
     4801 tcacgcccgc cggcctgcgt atggccggcg aggtcgcgga cggcaatttg ccgattttct
     4861 tttctcccga tgcgcccgat gtggtgacgg gtccgacgct ggagggtcgc cggaaagtcg
     4921 gcaagacgat ggagggtttc gataccgccc cgttcgtgcg ggccaaaatg ggtccggatg
     4981 tggcggcctg ccgcgatgcg atccgaccgg aactggcact gtatatcggc ggcatggggg
     5041 cgcgttcgaa gaacttctac aacgacatgg tcaccaagat ggggttcgag ggcgaggcga
     5101 gggtcattca ggacttctac ctcgacggca agaagaacga ggccgccgcg gcggtgccgg
     5161 acgcgctaat cgacgcgatt tctttatgcg ggccggctga acggatcagg gaccggttgg
     5221 cggcgtggaa ggatgtggcg aaggccggac atgtcgggac gatggtgctg aaggggtctg
     5281 gcgtggatgt gcttcgggtg gtggcggagg cggtgctgta gggacatgca aagccaccaa
     5341 cctaacccca taacgcgcgc agcccggcca ggggatccgc gtcgatcacc ggtgccagcg
     5401 ccttggcgat cgccaacaaa cgcccatccg ttaggcgctt gcccaccagc gtcacgccaa
     5461 ccggcaggtt ctttggccct cggcaacagg ggatgccgac attcggtcca tgcagcaacg
     5521 tccacatccg gttgaaaacg gcgtcgccgg tggtgtgcag cccctccggc gcctctccgc
     5581 aggagcttgg cgtcaggatc acgtccactt caggcccgaa catattgtcc agcactgggc
     5641 ggcaggcatc cgccagagcg tagctttcta gcagccgctg cggtgtgatc tgcaacgcgt
     5701 tctcgacctt ggccttcaaa tcggacgcca gaatgccatg cgcgttgacg tactccggca
     5761 gaaacgacgc cccgccttcg gcgtttacaa tcgcggtatg tgcatcgtgc aaccggttga
     5821 acggttccgg cagcaccagc tcgaaaaccc gagcaccggc cgccgccaac cgttcggcgg
     5881 cggtcgccag cgccatttcg ccaccctgct cgatttgacc ccacactggg ctttggcaca
     5941 ggccgacgcg aaggccctta acctggaccg gcgtcgtgtc gcctggcaac cggaacgcgg
     6001 agccaacgag gatcaggtcg tcgacgcagc ggccatacca accgacggta tccaatgtca
     6061 tcgaggacat ccgcacgccc tcccggctga caaggttcca tgacggttta agaccatata
     6121 tgccgttgaa cgacgcggga cggatatgcg atccgccagt ctgtgtaccg aatgccagcg
     6181 gcacctggaa atcgccgaca gcggcggccg aaccggacga cgacccgcca ggggtatggg
     6241 cag
//
LOCUS       DASWQN010000929         5272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2331610, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000929 DASWQN010000000
VERSION     DASWQN010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331610"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..351)
                     /locus_tag="VGC82_15870"
     CDS             complement(22..351)
                     /locus_tag="VGC82_15870"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY0424798.1"
                     /translation="MSLGLELKRRCRIMIAPSVFLAITGYFGWNATQGDRGLVAYAQR
                     QELAHKVVAEQNAARIERDGWETRVSGLRARHLNPDTLDERARSMLNLAEPNEVIVKL
                     GSKEKLF"
     gene            440..784
                     /locus_tag="VGC82_15875"
     CDS             440..784
                     /locus_tag="VGC82_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424799.1"
                     /translation="MSSEYNLCRTIEPDRPKEAFMGVRKLLLAALCASVALGTAGPAF
                     ADWDWHRHDRPDWHDRQEWRERQAWREREWREHEWRERREREFYRPPPVVAVPGYSVP
                     PPVYYGNPGYYR"
     gene            950..1789
                     /gene="dapD"
                     /locus_tag="VGC82_15880"
     CDS             950..1789
                     /gene="dapD"
                     /locus_tag="VGC82_15880"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY0424800.1"
                     /translation="MSQHALQQPIEDLWERRETLSSSTKGEARDAVEAALDALDAGQV
                     RVAEKHEAGWQVNQWLKKAVLMSFRLTDSEPMEGPGGAPVWDKVPMKFAGWGANRFKE
                     AGFRAVPGAVVRRSAFIAPGAVLMPSFVNVGARVGRNTMVDTWATVGSCAQIGANCHL
                     SGGVGIGGVLEPLQANPVIIEDDCFVGARSEVAEGVVVEQGSVLSMGVFIGASTKIID
                     RATGEIFMGRVPAYSVIVPGSLPGKKLPDGSPGPSLYCAVIVKTVDAQTRSKTSINEL
                     LRD"
     gene            1789..2949
                     /gene="dapE"
                     /locus_tag="VGC82_15885"
     CDS             1789..2949
                     /gene="dapE"
                     /locus_tag="VGC82_15885"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003618099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY0424801.1"
                     /translation="MTGSGLATQFIDPLPLAQSLIRCASVTPADAGAQDALTGMLARL
                     GFTIHRLRFGEIENVFARLGTAGPHFCFAGHTDVVPVGAANWRTDPFGGEVRDGVLYG
                     RGACDMKGAIAAFVSGVAQHLGQGVPKGSISLLITGDEEGPATDGTVKVLEWMRANDQ
                     VPDFCLVGEPTCPVRLGDMVKIGRRGSVNMKIEVHGTQGHVAYPHRADNPVHRLIQAL
                     GALTSAPLDAGTDWFEPSSLQVTSIDVGNVATNVIPASARAALNIRFNDRHSGASLTA
                     WVRATLARYAERFDLDALVSGESFVTKPGPMVDILRDAIAGATGLEPKLDTGGGTSDA
                     RFIANYCPVAEFGLVGATMHQTDERVPVAELRDLARIYRDIVAAFLALAPLA"
     gene            3002..3571
                     /locus_tag="VGC82_15890"
     CDS             3002..3571
                     /locus_tag="VGC82_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424802.1"
                     /translation="MSPGVVPGKSIFVGIFRIAYGRADGIACFGSSSQAFLSSLAPLL
                     AFPLVGAGLAIATEGPRRALTGLAMTLCALLTPPIVSYELSRIWHRTDAWIRFATAFN
                     WCEWILPLLAILLMVPLGVAISMGLNEAAASVVLFGCLALYGLWLHWFLARKALALPV
                     MRAVGLVFLVNLGTVAAVMGPRLLARGIG"
     gene            3568..4134
                     /locus_tag="VGC82_15895"
     CDS             3568..4134
                     /locus_tag="VGC82_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424803.1"
                     /translation="MSSVSTVTSGLTAAFRLARGQPDGAVTMMGDRNTIVRSFWAIAL
                     CLPAVVCRLLMSWAITGLPKDAIHLAGREVIVFVLGWLVFVELTHRLAPMIGRTDRWG
                     RFIVVWNWCNVIEGALIIVGGIPGMFGAPAIIDQAFELVSLGWALWLEWYATRLAFEV
                     SPLTAVGLVLLDQSIGILLASIAMSVTS"
     gene            complement(4124..4951)
                     /gene="truA"
                     /locus_tag="VGC82_15900"
     CDS             complement(4124..4951)
                     /gene="truA"
                     /locus_tag="VGC82_15900"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY0424804.1"
                     /translation="MTRWALLLEYDGTPFVGWQRQVNGLSVQEVLERAAAHLNASRPV
                     ASIVAGRTDAGVHAEGQVAHIELPDGHRAKTVREALNYHMKPHPVVVLAAVPVTDDWN
                     ARFAADRRSYRYRILDRPSRPALMVNRVWHVPNTLDAAAMHAAGQHLLGRHDFTSFRA
                     ASCQAKSPVRTLDRLDVTRRGDIVEVVTAARSFLHHQVRNFVGTLKLVGNGHWPVEHM
                     ITVLQAKDRAAAGPTAPSAGLTLIGVGYPDDPFRPGPYPEGLLPGSLLPSSLSPFRR"
     gene            complement(4948..>5272)
                     /locus_tag="VGC82_15905"
     CDS             complement(4948..>5272)
                     /locus_tag="VGC82_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424805.1"
                     /translation="AAAIDRQVRAFDPWPGTFTTLGGQPLKILAATIVDATGTPGTAI
                     DAGLTIACGDQAIRLTKLQLSGRGALEAGAFLRGHPVPPGTIFGPGPIVGSGATVGSG
                     TAPGQ"
BASE COUNT          871 a         1609 c         1785 g         1007 t
ORIGIN      
        1 tccgcccggc gtgatcacgc tctaaaacag cttttccttt gagccgagtt taacgatcac
       61 ctcgtttggt tcggcgaggt tgagcatcga ccgggcgcgt tcatccagag tatccgggtt
      121 cagatggcgg gcacggaggc cggaaacccg cgtttcccaa ccatcccgct cgatcctggc
      181 cgcgttttgt tcggctacga ccttatgcgc aagttcctgg cgctgagcat aggcaaccag
      241 gccgcggtcg ccttgcgtgg cattccagcc gaaataaccg gtaatcgcca ggaagacaga
      301 cggtgcgatc atgatgcggc accgccgctt cagttcgagg ccgagcgaca tcgcgttccc
      361 ccagggtttt gtctcgccgt tctagcgtgg cgacagcctc gggtgaacga aaaagccatc
      421 aagccgccag aatttcgtca tgtccagcga atacaatctt tgccggacga tcgagccgga
      481 tcgtccaaag gaggctttca tgggcgttcg caaacttcta ctggccgcgc tgtgcgcttc
      541 ggtcgcgctg ggtactgcgg gtccggcatt cgccgattgg gattggcacc ggcacgaccg
      601 gccggactgg catgatcggc aagagtggcg ggaacggcag gcatggcggg agcgcgaatg
      661 gcgcgaacac gaatggcggg agcgtcgcga acgggagttc taccgtcccc cgccggtcgt
      721 ggcggtaccc gggtattccg ttccgccacc ggtgtattac ggcaatcccg gctattaccg
      781 ctgaccgcgg agccgaacgg gatcaaccgg gtgcggtgcc tggggcacat gtgactgggg
      841 gcgtatgact ggggcgcgta tgactggggc gcgtatgact ggggcgcgtt gacaggacag
      901 gacgccgaac gcatcgtccg cccgccgcaa tccgggaaaa cgccaagcaa tgtctcagca
      961 tgccctgcaa cagccgatcg aagacctttg ggaacgtcgg gaaaccctct cttcatccac
     1021 caaaggtgag gctcgggatg cggtcgaagc cgcgctcgat gcgctggatg cgggccaggt
     1081 gcgggtcgcg gaaaagcacg aggccggctg gcaggtcaat cagtggctca agaaagccgt
     1141 gctgatgtcc ttccgcctta ccgactcgga gccgatggaa ggcccgggcg gagcgccggt
     1201 ttgggacaag gtgccgatga agttcgccgg ctggggagcg aaccgcttca aggaggcagg
     1261 cttccgcgct gtaccgggcg cggtcgtgcg gcgatcggcg ttcatcgccc cgggcgcggt
     1321 gctgatgcca agcttcgtca acgtcggtgc ccgggtcggg cgcaacacga tggtcgatac
     1381 ctgggcaacc gttggaagct gcgcgcaaat cggtgccaac tgccatttgt ccggcggagt
     1441 cggcatcggc ggcgtgctgg agccgctgca ggccaatccg gtgatcatcg aggatgattg
     1501 tttcgtcggt gcccgctccg aggtcgcgga aggcgtcgtc gtggaacagg gcagcgtgct
     1561 gtcgatgggc gttttcatcg gcgcgtccac caaaatcatc gaccgcgcga ccggagaaat
     1621 tttcatgggc cgggtgccgg cctattccgt cattgttccg ggctccttgc cgggcaagaa
     1681 gctgccggat ggctcgccgg gtccgtcgct ttattgcgcg gtcatcgtca agacggtcga
     1741 tgcgcagacg cgatccaaaa cctccatcaa tgagttgttg cgcgattgat gaccggatca
     1801 ggtttggcga cgcagtttat cgacccgctg ccgctggcac agtcgttgat ccgctgcgcc
     1861 tccgtgacgc ccgccgatgc cggcgcccag gatgccctga ccggcatgct ggcccggctt
     1921 ggattcacca tccaccggct ccggtttggc gagatcgaga atgttttcgc acgcctcggc
     1981 accgctgggc cgcatttttg tttcgccgga cataccgatg tcgtgccggt gggtgcagcg
     2041 aactggcgca ccgacccgtt cggtggtgaa gtgcgggatg gcgtgttgta tgggcgcggc
     2101 gcctgcgaca tgaagggtgc gatcgctgcc ttcgtttcgg gtgtcgccca gcatctcggg
     2161 cagggtgtgc cgaaaggctc catcagcctg ctgatcactg gcgacgagga gggaccggcg
     2221 accgatggca cggtgaaggt gctggaatgg atgcgggcga acgatcaggt tccggatttt
     2281 tgcctggttg gcgaacccac ttgcccggtg cggctgggcg acatggtcaa aatcggccgc
     2341 cgcggcagcg tcaatatgaa aatcgaggtg cacggcacgc agggccatgt cgcctacccg
     2401 catcgcgccg ataacccggt ccatcgattg atccaggcct tgggcgcgct cacctcggca
     2461 ccattggacg ccggaaccga ctggttcgaa ccgtcctcgt tgcaggtcac cagtatcgat
     2521 gtcggcaatg tcgcgacgaa cgtgattccg gcgtctgccc gagccgcctt gaacatccgg
     2581 ttcaacgatc ggcacagcgg cgcgtcgctg acggcgtggg tgcgcgcgac tttggcgcgg
     2641 tatgccgaac gattcgacct ggacgccttg gtcagcggcg aatcctttgt gaccaagccc
     2701 ggtcccatgg tcgatatcct gcgcgatgcc atcgccgggg cgaccggcct cgagccgaag
     2761 ctggataccg gcggcggaac gtcggacgcg cgtttcatcg ccaactactg cccggtcgcc
     2821 gaatttgggc tggtcggggc gacgatgcac caaaccgacg agcgggtccc ggtcgcggag
     2881 ttgcgcgatc tcgcgcggat ctatcgggac attgtcgcgg ccttcctggc gttagcccct
     2941 ttggcatgag ccccccttgg catgaattcc ttcgcatgag ccccagcatg aatcccttgg
     3001 catgagcccc ggtgtcgtcc cgggcaaaag tattttcgtg gggattttcc gcatcgccta
     3061 cgggcgggcg gacggcatcg cctgttttgg ctcaagctcc caggcgttcc tttccagcct
     3121 cgcaccgctg ctcgcgtttc cgctggtggg agcgggattg gcgatagcga ccgagggacc
     3181 tcgccgggcg ttgaccggtc tggcgatgac gctgtgcgcc ctgctgacgc cgccgatcgt
     3241 gtcctatgaa ttatcccgaa tctggcaccg gaccgacgcc tggatccgct tcgcgacagc
     3301 gttcaactgg tgcgagtgga tcctgccgtt gctggcgatc ctgctgatgg tgccgctggg
     3361 cgtggccatc agcatggggc tgaacgaggc cgcagccagc gtagtcctgt ttggatgcct
     3421 ggccttgtat ggcctgtggc tgcactggtt tctcgcgcgc aaagctcttg cgttgccggt
     3481 gatgcgcgcc gtgggactgg ttttcctggt caatctgggg accgtcgcgg ctgtcatggg
     3541 gcctcgtttg ctggcccgcg ggataggatg agttcggtct ccacggttac atccggcctg
     3601 accgccgcgt ttcgtctggc ccgcggccag ccggacggcg ccgtgacgat gatgggcgac
     3661 cgcaacacca tcgtgcgcag cttttgggct atcgcactgt gcctgcccgc ggttgtttgc
     3721 cggcttttga tgtcatgggc gatcaccggc ttgcccaagg acgcgatcca tctggccggc
     3781 cgcgaggtga tcgttttcgt attgggctgg ctggtgttcg tcgaattgac ccatcggctg
     3841 gcgccgatga tcggccgaac ggaccgctgg ggccggttca tcgtggtgtg gaactggtgc
     3901 aacgtgatcg agggcgcgct gatcatcgtc ggcggaatcc ccggcatgtt cggagcaccg
     3961 gcgatcatcg accaggcttt cgaactggtc agcctcggct gggcattatg gctggaatgg
     4021 tacgccaccc gcctcgcgtt cgaggtttcg ccgctgacgg ccgtggggct ggtgctgctc
     4081 gatcagtcga tcggaatcct gctggcgtcg atcgccatgt cggtcacctc ctgaacgggg
     4141 aaaggctgct ggggagaagg ctcccgggaa gaaggccctc cgggtaaggc ccgggccgga
     4201 aaggatcatc ggggtagccg acgccaatca gtgtcagacc cgccgaaggt gcggtcggtc
     4261 cggctgccgc gcggtccttc gcctgcaaaa ccgtgatcat gtgctcgacg ggccagtgtc
     4321 cgttgccgac gagtttgagc gtcccgacaa aattgcggac ctgatggtgc aggaagctgc
     4381 gggctgccgt caccacctcg acgatatcgc cccgccgcgt gacatcgagg cgatcgagcg
     4441 tccgcaccgg tgattttgcc tggcacgagg cagcccggaa cgacgtgaag tcgtgccgcc
     4501 ccagaaggtg ctgtccggcg gcgtgcatcg cggcggcgtc aagcgtgttc gggacgtgcc
     4561 acacccggtt gaccatcagc gcgggacggc tgggccgatc gagaatacgg tagcggtacg
     4621 acctccggtc ggcggcgaag cgggcgttcc agtcatccgt taccggaacg gcggccagca
     4681 cgacaaccgg atgtggcttc atatggtaat tcaacgcctc gcggaccgtt tttgcgcgat
     4741 ggccatcggg cagttcgatg tgggcgacct gcccctcggc atgaacgcct gcgtcggtgc
     4801 gcccggcgac gatgctggcg accggccggg atgcgtttaa atgggcagcc gcccgttcca
     4861 gcacctcctg aacagacaac ccgttgacct ggcgttgcca accgacgaac ggggtaccgt
     4921 cgtattccag cagcaacgcc caacgcgtca ttgcccggga gcagtgccgg aaccaacagt
     4981 cgcgccggaa ccaacaatcg ggccgggccc gaagatagtg ccaggcggaa ccgggtggcc
     5041 gcgcaggaac gcgccggctt ccagcgctcc gcggcctgaa agctggagtt tggtcagccg
     5101 gattgcctga tcaccgcagg cgatggtcaa gccggcgtcg atcgccgtac cgggcgtccc
     5161 tgttgcatcg acgatcgtag cggcaagtat cttgagtggc tgaccgccaa gcgtggtgaa
     5221 cgtgccgggc cagggatcga aagcacgaac ctgccggtcg atcgccgcgg ct
//
LOCUS       DASWQN010000930         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2068826, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000930 DASWQN010000000
VERSION     DASWQN010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068826"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..113
                     /locus_tag="VGC82_15910"
     CDS             <1..113
                     /locus_tag="VGC82_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072400.1"
                     /note="CysK; forms a complex with serine acetyltransferase
                     CysE; functions in cysteine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY0424806.1"
                     /translation="HTIVTILCDGGSRYQSKLFNPEFLRGKGLPVPPWMA"
     gene            complement(196..606)
                     /locus_tag="VGC82_15915"
                     /pseudo
     CDS             complement(196..606)
                     /locus_tag="VGC82_15915"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(613..867)
                     /locus_tag="VGC82_15920"
     CDS             complement(613..867)
                     /locus_tag="VGC82_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424807.1"
                     /translation="MEQESLARYDSVVAVSDRDRDVLAKRYGLPMVEAIDTGVDLDFF
                     AVNGAESEPDPGADVGTLIFTATMSWAANVEGIHFLLDEV"
     gene            complement(1361..1537)
                     /locus_tag="VGC82_15925"
     CDS             complement(1361..1537)
                     /locus_tag="VGC82_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424808.1"
                     /translation="MRRRRGHRLNLYGPRLSRGDDLGIHNRFVHAAKLMAPNRNYIEL
                     ACIRDLLAHPVICG"
BASE COUNT          343 a          584 c          509 g          269 t
ORIGIN      
        1 gccacacgat cgtgacgatc ctgtgcgacg gcgggtcgcg ctaccagtcg aagctgttca
       61 atccggagtt tctgcgggga aaagggctac cggtcccgcc gtggatggcg tgaagccggc
      121 tacttgggcg gcgcgacgtc ccgcggacga ccgccgccgc gcaagcgcgc gcaggcagat
      181 ggcttcgaac tgggctgcca cgcgcgacca ggagaaccgc tcctccatca agcggcgtgc
      241 cgcgccggcg atacgggcgc gcatcgaagc gtctgccagc agcgccagaa ccgcagaggc
      301 gaactccccg gcattgtccg cccgcaggaa atgttcgcca tcgatgacgt ccagcccctc
      361 gatccccagc gtcgtggaga ccacaggccg gcccatcgcc atggcctcga acgccttgat
      421 ccgggtccca ctgccgacga acaacgggat gatgtaaaca tttgccgcgg cgacgtaggg
      481 acgaatgtca tccacgaagc cggtcagcac cacgttcagg cgccgctgcc tgatcttgtc
      541 ggacaacgat gctgccggat cccgcccgat gatcaccgcc cgcattttgg gcctgacctt
      601 cagcagccga ggctaaacct catccaatag gaagtggatg ccttcgacgt tcgcagccca
      661 gctcatggtg gcggtgaaga tcagcgtgcc gacatctgct ccgggatcag gttcgctttc
      721 cgctccattc acagcaaaaa aatcgagatc gacgccggta tcgatcgcct ccaccatcgg
      781 caacccatag cgtttcgcca gcacgtctcg atcgcggtcc gataccgcga ccaccgagtc
      841 gtaacgggcc aggctttcct gctccaaccg gccatcttcc ggctttggtt ggcccagacc
      901 aagcctcgaa ttccacggac gcgcttggca tggcgttcga atatttcggc ttccacgttg
      961 tgggtgaaca tcaccagggc cgcgtccacc cgcgccggca caagcacatc ggcgtgcggg
     1021 aaatccacca ccacgacatc cggccgttcg gtcaaagcct gccgcaccac ggcactcccc
     1081 gccgccgacc gatccgtggc ggccgcgacc ggcagccggc tcgccagggc catcacccgc
     1141 cgcacccgcg accgcggctt ttccggccaa ctcacgaagc gatcgcaagc ggctgcgata
     1201 tcggccgcat acccagcatg atcgggcggc gcgggcgaaa ccagcgtcac gtggaaagcg
     1261 ccaccctgca ttgcccgcaa aatgttcccg gtgcggatct tgccgccctg atccatcgga
     1321 aacaaaaagc gcgggctgac gaacagcagc cgcggacggg tcacccgcat atgacgggat
     1381 gggccagcag gtcgcggatg caggccagct cgatgtagtt tcggttcggt gccatcagct
     1441 tcgcggcgtg cacgaagcgg ttgtggatac caaggtcatc accgcgcgaa agacgtggcc
     1501 catacagatt gaggcgatgc ccgcgtcggc gcctcacgtc ccgaaccggt gagacagccg
     1561 cggaaaccaa cccgccctgg acgacgtggg gatggcgacc tgggaatggc gacatcggaa
     1621 taaagattcg catacaagcg ccctcgtccg cgtgatcaca ctcttaccac acaccatcgc
     1681 gaggaaaaga aatcgctttg agttg
//
LOCUS       DASWQN010000931         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_747586, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000931 DASWQN010000000
VERSION     DASWQN010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747586"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VGC82_15930"
     CDS             <1..718
                     /locus_tag="VGC82_15930"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0424809.1"
                     /translation="FALTRGQSLRVPSHVWPRLLLFALLNVAGWMVLMGLALLWLPAS
                     EAALIAYTMPVWASLMAWPILGERPNLLRVISLVMAFAGISAIMGGNGFAASMDKLPG
                     ILMALGGAFGFALGTVLAKKFPLHLPPLAAAAWQIGIGCLPIAVIGFLFENAHVAAIT
                     RLGWWLLVYSTVIQFCVAYVSWFAALARLPASVAAIGTMAVPVIGVVASALALHEPLG
                     TGQIAALVFTLAGVALATRS"
     gene            812..1222
                     /locus_tag="VGC82_15935"
     CDS             812..1222
                     /locus_tag="VGC82_15935"
                     /inference="COORDINATES: protein motif:HMM:NF006040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEY0424810.1"
                     /translation="MKQFFLKFFTWWSGQTFGTQLWTWRFGELVGTDEQGNRYYRTIG
                     GKIDPSLGFERRWVVYNGYAEASRIPPSWHGWMHHTVDVPPVAEAYSPREWEKPHLPN
                     LTGTPYAYRPSGSTLASGRRPAATGDYQPWTPGN"
     gene            complement(1631..2317)
                     /locus_tag="VGC82_15940"
     CDS             complement(1631..2317)
                     /locus_tag="VGC82_15940"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY0424811.1"
                     /translation="MSTPLQIGLVIFPKVTQLDFTGPLQVFSNLPGATLHLIWKRIEP
                     VPSDSVLVLTPTATFADCPQLDVICVPGGAGTDDMVNDEEMLDFLRRQAESARYVTSV
                     CTGSLVLGAAGLLKGYRATTHWTAMDLLAPFGATPEKTRVCVDRNRVTGGGVTAGIDF
                     ALTLVSIMVNRQTAELIQLGLEYNPAPPFNSGSPDTASPEILALMKERIAPRRGRRVE
                     ANGRAAARLG"
     gene            2428..>2922
                     /locus_tag="VGC82_15945"
     CDS             2428..>2922
                     /locus_tag="VGC82_15945"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0424812.1"
                     /translation="MIGVLIFPGFQLLDAAGPISVFEIGARLAGQPPLIKALAVTSGP
                     VRSSSGVEMVARGLNAAGAISTLIVAGGEGVRSAAACGTTLAFVRKMAKRGIRIASVC
                     SGAFVLAEAGLLDGRRATTHWRRTLDFLAAYPKVKLEPDRIFVRDGNIWSSAGISAGI
                     DLALA"
BASE COUNT          434 a          945 c          987 g          556 t
ORIGIN      
        1 gttcgcgctc acgcgcgggc agagcttgcg ggttccatct catgtgtggc cgcggctgct
       61 gctgttcgcg ctgctcaatg tggcgggatg gatggtgctg atggggctgg cgctgctctg
      121 gcttccggcc agcgaggcgg cgctgatcgc ctacaccatg ccggtgtggg cctcgctcat
      181 ggcctggccg atcctcggcg agcggccgaa cctgctgcgg gtgatttcgc tggtcatggc
      241 gttcgccggc atcagcgcga tcatgggcgg caacgggttt gcggccagca tggacaagct
      301 gccggggatc ctgatggcgc tcggcggcgc gttcggcttc gcgctcggca cggttctcgc
      361 caagaaattt ccgctgcacc tgccgccgct ggccgccgcg gcctggcaga tcgggatcgg
      421 ctgcctgccg atcgccgtca tcggcttcct gttcgagaac gcccatgtcg cggcgatcac
      481 ccggctgggc tggtggctcc tggtctattc gaccgtgatc cagttctgcg tcgcctatgt
      541 cagctggttc gcagcgttgg cgcggctgcc ggcctcggtg gcggcgatcg gcaccatggc
      601 ggtgcctgtg atcggcgtgg tggcgtcggc gctggcgctg catgagccgc tcggcacagg
      661 ccagatcgcg gcgctggtct ttacgctcgc cggcgtggcg ctggcgacgc gatcgtgacg
      721 gttccggggc ctacggcagg cccgacttga ctggacaagg cctgccgccg atggcatcac
      781 gggccacagc ttctcgagcc ggcacgccga catgaaacag tttttcctca aattcttcac
      841 ctggtggagc ggccagacct ttggcacgca attgtggacc tggcggttcg gcgaactggt
      901 cggcaccgac gagcagggca atcgctatta ccgcaccatc ggcggcaaga tcgatccgtc
      961 gctcggcttc gagcggcgct gggtggtcta taacggctac gccgaggcgt cgcggattcc
     1021 gccgtcatgg catggctgga tgcaccacac cgtcgatgtg ccgccggtcg ccgaggccta
     1081 cagcccccgc gaatgggaaa agccgcattt gcccaatctg accggcacgc cctacgccta
     1141 ccgtccgtcc ggctcgacgc tcgccagcgg ccgccgtccc gcggccaccg gcgactatca
     1201 gccctggacg ccgggaaact aagcctcacg cattgtcatc gtcattccgg gttcgcgctt
     1261 tgcgcgcccc ggaatgacaa gacaacccct gtgaacagcg gggacatccg ccgacgcatt
     1321 gttgccgccc cgatggtctc atggctcaat gggtcatctt gcggagatgg gaaaccattg
     1381 cgtcggttcg ggccagttcg cgactgccac cgatttgcag cggcgcccga tcaggatacg
     1441 gctgggacct tcggtccaag gggctgctat cctgaaatcg cccgtcaatg tggcgccacc
     1501 gtaggacagg aaaggccgtc tgggaaacca ggcggccttt ttcttggcgt gcgggttctt
     1561 ggcgtgcggg ttcttggcgt gcgggttctt ggcgtgcgag ttcttggcgt gcggggctgc
     1621 gcgagaaagc tcagccgagc cgggcggcgg cgcggccgtt ggcttcgacc cggcgcccgc
     1681 ggcgcggcgc gatcctctcc ttcatcagcg ccaggatttc cggcgacgcc gtatccggcg
     1741 agcccgaatt gaacggcggc gcgggattgt attccagccc gagctggatc agctccgcgg
     1801 tctgccggtt gaccatgatc gagaccagcg tcagcgcaaa atcgatcccc gcggtgacgc
     1861 cgccgccggt gacgcggttg cggtcgacgc agacacgggt cttttccggc gtcgccccga
     1921 acggcgccag caggtccatc gcggtccagt gcgtggtggc gcggtagcct ttgagcagtc
     1981 cggccgcgcc cagcaccagc gaacccgtgc agaccgaagt gacgtatctc gcgctttcag
     2041 cctggcggcg caggaagtcc agcatctcct cgtcattgac catgtcgtcg gtgccggcgc
     2101 cgccgggcac gcagatcacg tcgagctgcg gacaatcggc aaaggtcgcg gtgggcgtca
     2161 gcaccagcac ggaatcgctc ggcacaggct cgatccgttt ccagatcaga tgcagcgtcg
     2221 cgccgggcag gtttgaaaac acctgcaacg gcccggtgaa atcgagctgg gtcaccttcg
     2281 gaaagatcac aaggccgatc tgaagcggcg tggacatcgc gaggtccttt cgctggtgcg
     2341 cggtttcact tgaccgctcc aatgtctcat gatcggatgt tgtccgaaat ggcataattc
     2401 cctcatttta ggacatgatc tgaaatcatg atcggcgtgc tgatctttcc cggttttcaa
     2461 ctgctcgacg ccgccggtcc gatttcggtg ttcgagatcg gcgcccgcct ggccggccag
     2521 cctccgttga tcaaggcgct ggcggtgaca tcaggtccgg tccgcagctc ttccggcgtc
     2581 gagatggtcg cgcgcggcct caatgccgca ggcgccatca gcacgttgat cgtggcggga
     2641 ggcgaggggg tgcgaagcgc cgcggcctgc ggtacgacgc tggccttcgt ccgcaagatg
     2701 gcaaagcgag gcatccgcat cgccagcgtc tgctccggcg cgtttgttct ggccgaggcc
     2761 ggcctgctcg atggccgtcg cgccaccacg cactggcggc gcaccctgga ttttctggct
     2821 gcctacccga aggtgaagct ggagccggac cggatcttcg tccgcgacgg caatatctgg
     2881 agttcggccg gcatcagcgc cggcattgat ctggcgctgg cg
//
LOCUS       DASWQN010000932         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_881517, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000932 DASWQN010000000
VERSION     DASWQN010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881517"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VGC82_15950"
     CDS             complement(<1..328)
                     /locus_tag="VGC82_15950"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0424813.1"
                     /translation="MIQNADEVVRGAVVNANICIVGGGPAGITLAMELARTGQSILLL
                     ESGDLGPTTEAQALNAGEVVDEALHSPPDKYRQRCFGGGTSIWGGRCVPFDPIDFEAR
                     SWVEHSG"
     gene            complement(325..1461)
                     /locus_tag="VGC82_15955"
     CDS             complement(325..1461)
                     /locus_tag="VGC82_15955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0424814.1"
                     /translation="MSDRRTVFLACPYGQVGGGMGSIMAYLAAMRQDPSGRFDLKRLE
                     SRGGGHLALSPFFLALAVGRIFKEAAQGRLAVVHLNLAERGSVYRKAVLLAATKLVGG
                     RVLLHLHAAQIVQFHASMGRTGQALLRWMFRSADHCVVLGEVWRRWVIDTFAVRPNRI
                     GVIYNGVPATVARARGLRDDGRFRVLFVGNLLERKGVSDLLRAIAMPCMMARGVSLIM
                     AGGGPVETYRSMASDLGIADRVTFTGWVSQDDARALMVNSDALILPAYDEGLPLVILE
                     ALASGTPVICTPVGSIPEVLEDRHTALFVAPGDKAGIAKAITTLLDQPLVAEQLSKAG
                     AALYNRLFTMEAFAGAVGSLYAALTPRDGSMTPSVWPPVEEPKR"
     gene            complement(1458..2270)
                     /locus_tag="VGC82_15960"
     CDS             complement(1458..2270)
                     /locus_tag="VGC82_15960"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0424815.1"
                     /translation="MDQMRAALNDVDCVVNCIAGSASTMVQSTRVLCDAARTSPPRRM
                     IYLSSMAVYGGAAGSVREDHSPVAPVSGYGQAKLECERMVRKYVDDGGEAVILRPTCV
                     FGPGSPQWTTRLAHLLRAGRIGDLGIAGDGCCNLAFIDDVVAAIVTALDAPDVSGRTF
                     NLSSSSELSWNEFFVAFAKAIGATPVRRISPRSLQIETRLLAPMRRIAAMALRSPATE
                     AITPSLVALWGQNIQIDCTAARAVLSLPRTNLERMIAAVVRDERPLKQSALP"
BASE COUNT          459 a          790 c          719 g          386 t
ORIGIN      
        1 aacccgaatg ctcgacccag gatcgcgctt cgaagtcgat cggatcgaac ggaacacagc
       61 ggccacccca gatcgaagta ccgccgccga aacaacgttg gcggtattta tccggcgggc
      121 tgtgcagcgc ttcgtccacg acctcgccag cattcaaagc ctgtgcttcg gtggtgggtc
      181 caaggtcgcc cgactcgagc agcaggatgc tttggccggt gcgggcaagt tccatcgcca
      241 aggtgatccc ggccgggccg ccgccgacaa tgcagatatt ggcgttgacg acggcgccac
      301 gaacaacctc gtccgcgttc tgtatcatcg cttaggttcc tcgaccggcg gccacaccga
      361 tggtgtcatg cttccgtctc gcggcgtcag tgccgcatac aacgaaccga cggcgccggc
      421 gaacgcctcc atggtgaaca gccggttata caatgcagcg ccagccttcg agagttgttc
      481 cgcgacaagc ggttggtcga gcagggtggt gatcgccttc gcgatgcccg ccttatcgcc
      541 cggcgcgacg aacagcgccg tgtgacggtc ttccagcact tccggtatcg agcccacggg
      601 ggtgcagatc accggcgttc cggaggccag cgcctccagg atcaccagcg gcagtccttc
      661 atcgtaggca ggcaggatca gtgcgtccga attcaccatg agggctcggg catcatcctg
      721 gctcacccat ccggtgaagg tgacgcgatc ggcgatgccg aggtccgagg ccatactgcg
      781 ataggtttcg accgggccgc cccctgccat gatcaggctg acgccgcggg ccatcatgca
      841 aggcatcgcg atagcccgca gcaggtcgct cacgcccttg cgttccaaca gattgccgac
      901 gaacaacacc cggaaccgcc catcatcccg caagccgcgg gcgcgcgcga ccgtcgccgg
      961 cacgccattg taaatgaccc cgatgcggtt cggccgcacc gcgaacgtgt cgatcaccca
     1021 gcgccgccac acttctccga gaaccacgca gtgatccgcg ctgcgaaaca tccatcgcag
     1081 caaggcttgt ccggttctgc ccatggaggc gtggaactgc acgatctgcg ctgcgtgcag
     1141 gtgcagcagc acgcgcccgc cgaccaactt ggtcgcagcc aagagaacgg ccttccgata
     1201 gacgctgcca cgttccgcca ggttcagatg aaccacggcc agccggccct gcgccgcctc
     1261 cttaaaaatt cgtccgacgg ccaaagccag gaaaaacggc gacaacgcga gatgtccgcc
     1321 accgcgcgat tccaggcgct tcagatcgaa acggcccgac ggatcctgcc gcatggcagc
     1381 gaggtaggcc atgatgcttc ccatgccgcc gccaacctgc ccataggggc atgccaggaa
     1441 caccgtgcgg cggtcactca tggaagcgcc gattgcttca acgggcgttc gtcccgtacc
     1501 accgcagcga tcatccgctc caggtttgtt cgcggcagcg acagtaccgc ccgtgccgcg
     1561 gtgcaatcga tctgtatgtt ctggccccac aaagcaacga gcgacggtgt gatcgcctcg
     1621 gttgccggtg aacgcagcgc catcgccgca atccggcgca tcggggcaag cagcctggtc
     1681 tcgatctgca atgagcgcgg cgagattcgg cgaaccgggg tcgcgccgat ggctttggcg
     1741 aaggcaacga aaaattcgtt ccaggaaagt tccgatgagc tggaaaggtt gaaggtccga
     1801 cccgacacgt cgggtgcatc cagcgcggta acgatcgccg ccacgacatc gtcgatgaac
     1861 gcgaggttgc agcagccgtc gcccgcgatc cccaggtcgc cgatgcggcc ggcgcgcagt
     1921 aggtgggcca gtcgcgtcgt ccattgtggg ctgcccggtc caaacacgca ggtcgggcgc
     1981 aaaatgacag cctcgccgcc gtcgtccacg tatttgcgca ccatgcgctc acattccagc
     2041 ttggcctggc cgtagccact gacaggagcg accggggaat ggtcttcgcg aaccgaaccg
     2101 gccgcgccgc catagaccgc catgctgctc aaataaatca ttcggcgcgg tgggctggtt
     2161 cgggcggcat cgcagagaac ccgggttgac tgtaccatag tgctggcact gccggcgatg
     2221 cagttgacca cgcagtcaac gtcattcagc gccgctcgca tttgatccat atggttgcat
     2281 cgacagccag cgcggagcgg cgagaagccg ccactgcccg atagatcggg ctgtcggcca
     2341 aggctgcaac cacc
//
LOCUS       DASWQN010000933         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_617426, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000933 DASWQN010000000
VERSION     DASWQN010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617426"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1727
                     /locus_tag="VGC82_15965"
     CDS             <1..1727
                     /locus_tag="VGC82_15965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0424816.1"
                     /translation="RMQGKLKLRWFPNLSLKVLPPVRLDVPATLVGRTRRAALGRIMQ
                     DIMTNAAFRPERLRHSLFGALLAAQRSYDKGLKVIADIVPNEAGDGNTITELSYRRLI
                     LGSVILGRKLAALTRPGEHVGIMLPNAAGTVVTFFGLQSQARVPAMMNFTSGADGMLS
                     CCTAAGIRTILTSRRAVQKGKLDRLVEGVATRVRFVYLEDIRASIGLLDKLIGMLVAR
                     RAERLPGADLDAAAPALVLFTSGSEGTPKGVVHSHRSLLANCAQLRAIVDFNPTDKVF
                     NALPMFHSFGMTATLLPLMYGVRTFLYPSPLHYKLVPEMVYAEQSTIMFGTDTFLTGY
                     ARKGHALDFQSLRYIFAGAEKVRPETRAAYMAHFKKPIFEGYGTTETAPVLALSTWAH
                     TKAGSVGRLLPGIEATLEPVPGISEGGRLLVRGPNVMLGYLRTEAPEMLQPPPDGWYD
                     TGDIVTIDNDGFVAITGRAKRFAKIGSEMVSLAAAEDLANAAWTDAVNAVVAVPDERK
                     GERLLLITTQRNADHRGLLVVARERGAAEIQVPRDIMVVEKLPMLGTGKIDYPAVQRL
                     AERPKVVA"
     gene            complement(2070..>3168)
                     /locus_tag="VGC82_15970"
     CDS             complement(2070..>3168)
                     /locus_tag="VGC82_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0424817.1"
                     /translation="GCDTIALVLQGGGALGAYQAGIYQAMHEAGLWPDTIAGVSIGGI
                     NAAIIAGNPPERRIDRLREFWEGITARPVSPFALDGLAARKATNIWSAFFTTTLGQPG
                     FFTPNVPNPWISVRGSKTATAFYDTTPLRETLVRLIDFNLLNNGTVRYAAGAVNVTSG
                     NFAYFDSTQEKIGLEHVMASAALPPAFPAVQIGPESYWDGGLVSNTPLQHVLDNAASL
                     HLLIFQVDLFSARGVPPRDMDDVLARQKDIQYSSRTRLVTDYFRDKHNRDVEAKKLLE
                     KIPDDRLDAAERALKARLAHMPEATILEMIYQKAAYETQAKDYEFSGASMREHWNSGY
                     QDMQCTLLHRSWLNVAESATGLRVHDIHRGR"
BASE COUNT          557 a         1022 c         1012 g          577 t
ORIGIN      
        1 cacggatgca gggcaagctg aagctgcgct ggttcccgaa cctgagcctg aaggtgctgc
       61 cgccggtgcg cctcgatgtg cctgccacgc ttgttggccg gacccgccgc gcggcgctag
      121 gccggatcat gcaggacatc atgaccaatg ccgccttccg cccggagcgc ctgcggcaca
      181 gcctgttcgg cgccctgctg gcggctcagc gctcatacga caagggcttg aaggtgatcg
      241 ccgacatcgt tccgaacgag gccggagacg gcaacacgat caccgaactg tcctaccgcc
      301 gtctgatcct tggcagcgtc atcctcggcc gcaaactggc ggcgctcacg cgccccggcg
      361 agcacgtcgg catcatgttg ccaaacgcgg ccggaaccgt ggtgaccttc ttcgggctgc
      421 agtcgcaggc acgcgtgccg gcgatgatga actttacgtc cggcgccgac ggtatgctgt
      481 cctgctgcac cgctgccgga atccgaacaa tcctgacgtc acgccgagcc gtgcagaagg
      541 gcaagctgga tcggctggtg gagggtgtcg cgaccagagt ccggttcgtt tacctggagg
      601 acatccgcgc ctcgatcggg ttgctggaca agctgatcgg catgctggtc gcccgtcggg
      661 cagagcggtt gccgggcgcc gatctggacg ccgccgcccc ggcgctggtg cttttcacct
      721 cgggttccga gggcacgccc aaaggcgtcg tccacagcca ccgctcgctg ttggccaact
      781 gcgcgcagtt gcgtgcgatc gtcgatttca acccaaccga caaggtgttc aacgcgctgc
      841 cgatgtttca ttccttcggc atgaccgcca cgctgctgcc gctgatgtat ggcgtgcgca
      901 cgttcctgta tccgtcgccg ctgcactaca agctggttcc ggaaatggtt tatgccgaac
      961 aatccaccat catgttcggc accgacacgt tcctgaccgg ttacgcgcgc aaaggccacg
     1021 cattggattt ccagtcgctg cgctacatct tcgccggcgc ggaaaaagtg cggccggaaa
     1081 cccgcgctgc ctacatggcg catttcaaga agccgatctt cgaaggctac ggcacgacgg
     1141 agaccgcacc cgtgctggcc ctgagtacat gggcgcacac aaaggcgggt tccgtcggac
     1201 gcctgttgcc cggcatcgag gcaacgctgg agcctgttcc cggcatcagc gaaggcggcc
     1261 gtcttctggt gcgcggtccg aacgtgatgc tgggctacct ccgaacggag gctccggaga
     1321 tgctgcaacc tccgccggat ggctggtacg acaccggcga catcgtgacg atcgacaacg
     1381 acggttttgt cgcgatcacc gggcgcgcca aacgcttcgc caagatcggc agcgaaatgg
     1441 tctctcttgc cgccgcggag gatctggcga acgccgcctg gaccgatgcc gtcaacgcgg
     1501 tcgtcgccgt gccggacgaa cgaaaggggg aaagactgct gctgatcacc acccaacgca
     1561 acgccgatca caggggactg ctggtggtgg ctcgggagcg cggggcagcc gagatccagg
     1621 ttccgcggga catcatggtg gtcgagaaac tgccgatgct ggggactggg aagatcgatt
     1681 atcccgcggt gcagcggctg gccgagcggc ccaaagtcgt cgcataacgc cgagctgctg
     1741 ctcaaatccg atcgccgcct tcaccttcgc cgccgagatc ggcagatccc gcaaccgaac
     1801 ctccgccgca tcgcctcggc cattgcggag gcggccggcc cctgccccgc ttcatccgcc
     1861 acctcaatgc ccacgatggc aaagcctcca ttgcaagagc gacgagactt ccggcgggcg
     1921 gtgtggccag gcgtgatcac gccgactgga aaaggtgccg gccagttcgc cgcctgacgc
     1981 ccatgtgtca tggccgtgcc tggcgcggcc acccacgact tcctttgcag ccgcgagcga
     2041 acgctgtttc cagccaccgt gaaaccgcgc tagcgacctc ggtgaatgtc atgaacccgc
     2101 agcccggtgg cgctctcggc gacgttcaac catgaccggt ggagcagcgt gcactgcatg
     2161 tcctgatagc cactgttcca atgttcccgc atcgacgcgc cgctgaattc gtaatccttt
     2221 gcctgggtct cataggcggc tttctggtag atcatttcca ggatcgttgc ctcgggcatg
     2281 tgcgccagcc gtgccttcag ggcgcgctcg gcggcatcca gccggtcgtc ggggatcttt
     2341 tccagaagct ttttcgcctc tacgtcgcgg ttgtgcttgt cacggaaata gtcggttacg
     2401 agacgcgtgc ggctggagta ctggatatcc ttctggcgtg ccagcacgtc gtccatgtcg
     2461 cgcggcggca ctccgcgggc gctgaacagg tcgacctgaa agatgagcaa atggagactc
     2521 gcggcattgt ccaacacatg ctgcaagggc gtgttggata ccaggccgcc atcccaatac
     2581 gactcggggc cgatttgcac cgcgggaaag gccggcggca gggctgcgct cgccatcaca
     2641 tgctccaagc cgatcttttc ctgcgtgctg tcgaaatagg cgaagttgcc gcttgtgacg
     2701 ttgaccgcac cggcggcgta gcggaccgtc ccgttgttca gcaggttgaa atcgatcagg
     2761 cgcaccagcg tctcgcgcag cggggtggtg tcgtagaagg cggtcgcggt tttagacccg
     2821 cgcacgctga tccatgggtt cggcacgttg ggcgtgaaga agccgggttg cccaagcgtg
     2881 gtggtgaaaa aggcggacca gatgttggtg gccttgcggg ctgcgaggcc gtccaaggca
     2941 aatggcgaca ccggacgagc ggtgatgcct tcccagaact ctcgcagccg atcgatccgg
     3001 cgttcgggcg gattgccggc aatgatcgcg gcgttgatgc cgccaatcga tacaccggcg
     3061 atggtgtccg gccacaatcc agcctcgtgc atggcttggt agatgccggc ctggtaggca
     3121 cccaatgcac cgccaccttg caggaccaac gcgatcgtgt cgcagcct
//
LOCUS       DASWQN010000934         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2464235, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000934 DASWQN010000000
VERSION     DASWQN010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2464235"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VGC82_15975"
     CDS             <1..987
                     /locus_tag="VGC82_15975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY0424818.1"
                     /translation="DLSGRVAVTCFASNVARMESVALAAQDCGRSVALIGRSLRNMEA
                     AARECGYMKTLPPFLTEDDIDDIPDENILMLITGSQGEARSALSRIARDDHPRVSLGE
                     GDTVIYSSRMIPGNERAIGTVQDNLVRRGVRLMTDDDHLVHVSGHPARDELRRMYRLV
                     KPRYAIPVHGEWRHLSAHAELAREAGSIPFMMEDGDIITLSPGRPEISDSAPVGRLVL
                     DGTRLVPLKGEVMSARRRMLFNGIVVASVAVDQAGTLRGRPKISAPGLLDPDDPDTDR
                     VADDFAETLRDLPASLRREDAGLEDAARSALRRALGRKLGKRPMVDVHLIRV"
     gene            994..1254
                     /locus_tag="VGC82_15980"
     CDS             994..1254
                     /locus_tag="VGC82_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HEY0424819.1"
                     /translation="MNWFTGVVLFVIIWWTALFAVLPIGTTPVARADDTSGWRGAPEH
                     PRLWRKVIVTTIVASVLWSASYLLIRSDYVSFRHGMFAEPDD"
     gene            1637..>2075
                     /locus_tag="VGC82_15985"
     CDS             1637..>2075
                     /locus_tag="VGC82_15985"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974690.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY0424820.1"
                     /translation="MRLSRGFIPTLKETPNEAQIASHRLMLRAGLVRQTSAGIYAWLP
                     LGLRVLRNIERIVREEQDRAGAQEILMPTIQPAELWRESGRYEDYGKEMLRIRDRHDR
                     DMLYGPTNEEMVTDLFRQSVKSYRELPQILYHIQWKFRDEVRPR"
BASE COUNT          378 a          649 c          660 g          388 t
ORIGIN      
        1 gacctgagcg gacgtgttgc tgttacctgc ttcgccagca atgttgctcg gatggaatcg
       61 gtagcgctcg ccgcccagga ttgcggccgc agtgtggccc tcatcggccg gtcgctgcgc
      121 aacatggagg ctgccgcccg cgaatgcggt tacatgaaga ccctgccgcc gttcctcacc
      181 gaggacgaca tcgacgacat ccccgacgag aacattctga tgctgattac cggcagccag
      241 ggcgaagccc gcagtgcatt gtcacgcatc gcgcgggacg accatccacg ggtctcgctg
      301 ggagaaggcg acaccgtcat ctactcctcg cgcatgatcc cgggaaacga gcgcgccatc
      361 ggcacggtgc aggacaacct ggtgcggcgc ggcgtccggc tgatgaccga cgacgaccac
      421 ctggtgcatg tctccggtca tccggcgcgc gatgagcttc gccgcatgta ccgtctggtc
      481 aagccgcgct atgccatccc ggtgcacggc gagtggcgcc acctgtcggc tcatgccgaa
      541 ctggcgcgcg aggcaggctc gatcccgttc atgatggagg acggcgacat catcacgctg
      601 tcgcctggcc gtcctgaaat cagcgacagc gccccggtcg gacgcctcgt gctggacggc
      661 acccgtctgg tgccgctgaa gggcgaggtg atgtccgcga ggcgccgcat gttgttcaac
      721 ggcatcgtcg tcgccagcgt cgcggtggat caggccggca ccctgcgcgg ccgtccgaag
      781 atcagcgccc cgggcctgct ggacccggac gacccggaca ccgaccgggt ggcggacgat
      841 ttcgccgaaa cgctgcgcga tctgcccgcg agccttcggc gtgaggacgc aggcctcgag
      901 gatgcggctc gttcggcgct tcgccgggca ctggggcgga aactgggcaa gcgcccgatg
      961 gtggacgttc atcttattcg ggtctgaacg aagatgaact ggttcactgg cgtcgtactg
     1021 tttgtgatca tctggtggac cgcacttttc gcggttctgc cgatcggaac cacgccggtg
     1081 gcgcgggcgg acgacacgag cggctggcgc ggcgcacccg aacacccgcg attatggcgc
     1141 aaggttatcg tcaccacgat cgtcgcgagc gttttatgga gcgcttcgta tcttctgata
     1201 cgaagcgact acgtgagttt ccggcacggc atgttcgccg aaccggatga ttgatgtcga
     1261 tttttcggag ttagtggcga actgccccgc ctcggcggac ggaggaatgt gtcctcggtc
     1321 cgccgggcgg atggcgtttc ctccccaaac ctggcccgct tacgcgctgg taagcagccc
     1381 aggccttgtt agccaaaccc tgcgatgaag tcactgacat ttggcaaatt cctatctgat
     1441 gacgtagatt ttattgcatg ccgggattgt gcaatgcaca aaaatgacga tgtcgctgca
     1501 cattttgtcg tccgttcgct cttccgtgaa gcacagccca tccgtctcaa acggctttgg
     1561 gcatcacgaa ccggtagcag aattggccca aacgggttac caccacgcgt tacacttcga
     1621 atggaaatcc atcgcgatgc gtctgtcccg cggctttatc cccacgctga aggaaacgcc
     1681 gaacgaggcg cagattgcct cccaccggct gatgctgcgg gcggggctgg tgcgccagac
     1741 ctcggccggc atctatgcct ggctgccgtt ggggctgcgc gtgctgcgca acatcgaacg
     1801 catcgtgcgc gaggaacagg acagggcggg cgcgcaggaa atcctgatgc cgaccatcca
     1861 gcctgccgaa ttatggcgcg aaagcggacg gtatgaggat tatggcaagg agatgctgcg
     1921 gatccgcgac cggcacgacc gcgacatgct gtatggcccg accaatgagg agatggtcac
     1981 cgatcttttc cgccagtcgg tcaagagcta ccgcgagctg ccgcagatcc tgtaccacat
     2041 tcagtggaaa ttccgcgacg aggttcgccc ccgct
//
LOCUS       DASWQN010000935         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1144674, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000935 DASWQN010000000
VERSION     DASWQN010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144674"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..265
                     /locus_tag="VGC82_15990"
                     /pseudo
     CDS             92..265
                     /locus_tag="VGC82_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009870292.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            361..1533
                     /locus_tag="VGC82_15995"
     CDS             361..1533
                     /locus_tag="VGC82_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY0424821.1"
                     /translation="MEKQTAVAPTAVAGELVRIFLALELSRSSWLVASHTPAADKISR
                     HKLAAGDIDGLLALIERLRTRVEQKTGQPVQVISCYEAGYDGFWLDRKLKAEGVINHV
                     MDPASIQVDRRARRVKTDAVDADALLRALMAFCRGEKKVCSMVRVPTRDEEDAKRLSR
                     EREQLIKERVRHVNRIKGLRATQGIYDYQPLKANRLERLDELCTGDGNPLPDRLKNEI
                     RRQLERLKLLLELIGTVEAERDAVVKADKPASEADRRIRALAKLRGIGPEIATVLYNE
                     VFYRQFANRRDVAAYVGLTPSLFASGDLRHDQGISKAGNPRARATMVELAWLWVRHQS
                     GSALTAWFLARVSGIKGRIKRIAIVALARKLLVALWRYLETGLVPTGAMLKADKQG"
BASE COUNT          363 a          546 c          596 g          329 t
ORIGIN      
        1 cttgccatct atcagtttga gcagaaggtt gttgacgtga cgaatcggtg tgtggttcaa
       61 ggaatcggcc tttggcggaa cctggaagcg catcatggac ggcatcacgg aaattgcggg
      121 cagcttaggc gaggaactca agcgtcgcct gccgaaacag cgtaagaccc agcggacgaa
      181 actggctctt ctagttgcga cgatgctcga cgtgcgcagt gccaacctga tggacttggc
      241 tgccgggctg cctcgggagg ctgaccgcac cgggccttta gatcctattg gggcgtgctc
      301 aggccgggac gattagactc acggcatcga tcgctgacca aacgctctgg agatcttgtc
      361 atggaaaaac agaccgctgt tgcgccgact gccgttgccg gagagctcgt ccggattttt
      421 ctggccctgg aactgagccg ctcgagctgg cttgtcgcca gccacacgcc ggccgccgac
      481 aagatcagcc ggcacaagct tgccgccggg gatatcgatg gcctcctggc cctgatcgag
      541 cgtctgcgga cacgggtcga gcagaaaacc gggcagccgg tccaggtcat ctcgtgctac
      601 gaggccggat acgatgggtt ctggctggat cggaaactga aagcggaagg tgtcatcaac
      661 catgtgatgg atccggcaag catccaggtc gatcgtcgag cccggcgggt caagaccgac
      721 gccgttgatg ccgacgcact gctgcgcgcc ctgatggcct tctgccgcgg cgaaaagaag
      781 gtgtgcagca tggtgcgggt gccgacccgg gacgaggagg acgcaaagcg cctcagtcgg
      841 gagcgcgagc agctgatcaa ggagcgcgtc cggcatgtca accggatcaa gggcctgcgc
      901 gccacccagg ggatctacga ttatcagccc ttgaaggcga accgcctgga acggctggac
      961 gagctgtgca ccggcgacgg caaccctctg cccgaccgcc tcaagaacga gatccgccgc
     1021 cagctcgagc gcctgaagct tctgcttgag ttgatcggta ccgtcgaggc cgagcgcgac
     1081 gccgtcgtca aagccgacaa accggccagc gaggcggacc gcaggatccg cgcgctggcc
     1141 aagctccggg gcatcgggcc ggagatcgcg acggtgctgt acaacgaggt gttttaccgg
     1201 cagttcgcca accggcgcga cgtcgcggcc tatgtcggat tgacgccgag cctgttcgcc
     1261 agcggcgatc tgcgccacga tcagggcatc agcaaagctg gcaacccgcg ggcgcgggcg
     1321 acgatggtcg aactggcctg gctgtgggtg cgccatcagt ccggcagtgc cctgaccgct
     1381 tggttcctgg cccgcgtgag cgggatcaag ggccgcatca agcgcatcgc catcgtcgct
     1441 ctcgctcgca agttactggt cgcgctctgg agatatctcg agaccgggct ggttccgacc
     1501 ggagcgatgc tgaaggctga caaacagggg tagccgagcc gatcggtgaa atccccaagc
     1561 cgtccaaggt ggatgtgtga ccgtgcgttg ccatggctgg aatacgccga cctttagatt
     1621 ggtcccatcc tcttggagcc cacctgtcct ccgcgcatga gggattatgg ttccggatca
     1681 ctgcgatccg accggatata aggtgatgcg gatctttgtt ccgcatgatg tgtcctgggc
     1741 tccacccttg gtcggctaac caaccagatc ggaaggatat catgcatcac tgaaactctt
     1801 gatatgaaac gcctcatata aggcaacgcc ggag
//
LOCUS       DASWQN010000936         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1673476, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000936 DASWQN010000000
VERSION     DASWQN010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673476"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /gene="lepB"
                     /locus_tag="VGC82_16000"
     CDS             complement(<1..596)
                     /gene="lepB"
                     /locus_tag="VGC82_16000"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY0424822.1"
                     /translation="MATTKASSRRQSSAWMENIKTIVYAGLIAVGVRTVAFEPFNIPS
                     GSMIPTLLVGDYLFVAKYSYGYSRFSLPFSPPLFSGRIFESLPKRGDVVVFKYPRDTS
                     IDYIKRIIGLPGDRIQVRAGQLYINGELCPRQSQGDYLADDSGIRMMRKLYQETLPNG
                     VKHELLKERDDGWVNNTQEYLVPAGHVFAMGDNRDNSA"
     gene            complement(767..1174)
                     /gene="acpS"
                     /locus_tag="VGC82_16005"
     CDS             complement(767..1174)
                     /gene="acpS"
                     /locus_tag="VGC82_16005"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY0424823.1"
                     /translation="MILGIGSDICDIRRIEKAIERHGARFLERVFTDVERSKAMRRTE
                     KIQAGTFAKRFAAKEAGAKALGTGFRRGVFFSNLGVVNLPGGQPTLLMTGGSAERLRA
                     ITPAGMVAQVHLTMTDEYPYAYAHVIISAIPAT"
     gene            complement(1249..>2172)
                     /locus_tag="VGC82_16010"
     CDS             complement(1249..>2172)
                     /locus_tag="VGC82_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT AH/RIS domain-containing protein"
                     /protein_id="HEY0424824.1"
                     /translation="DTCVGAKVNGRLMPLRHHLENGDQVEVMTARGGTPSPQWDRFVV
                     TGKARARIRRFIHQEQRQQTRDAGRVDLTKAFRQAGVDGSEKALEPALKALKLATVDD
                     LYVAVGNGNLIAKDVVHAAYPELRQAPRTSRMVPPMLPRGKTPTRHDHDMPVTGLVPG
                     MAYTFAGCCHPVPGDEIVGIVTTGKGVTIHGRECQTLTAFAATPERFIDVDWNYEAVG
                     KAGGESKGFGHTARISAIAANEQGALADISNAIAKQEGAIANLKIVNRQQDFMEVLVD
                     VDVRDIAHLSKVIVGLRGLKTIKGAERATGG"
BASE COUNT          384 a          693 c          677 g          418 t
ORIGIN      
        1 tcggcgctgt tgtcgcggtt gtctcccatg gcgaagacgt ggccggccgg aacgaggtat
       61 tcctgcgtgt tgttgaccca gccgtcatcc cgctctttca gcaactcgtg cttgacgcca
      121 ttcggcagcg tttcctgata cagcttccgc atcatcctga tgccgctgtc gtcggccagg
      181 tagtcgcctt gcgactgacg gggacaaagc tcgccgttga tgtagagctg gccggcacgc
      241 acctgaatac ggtcgccggg caggccgatg atacgcttga tatagtcgat cgaggtatcg
      301 cggggatact tgaacaccac cacatcccct cgcttcggca gcgactcgaa gatccggcct
      361 gaaaacaggg gcggcgagaa cggcagggaa aaccgcgagt aaccgtagct gtacttcgcc
      421 acgaacaggt agtcgccaac caacaaggtc gggatcatgc tgccggaggg gatgttgaac
      481 ggctcgaacg ccacggtgcg aacgccgacg gcgatcagtc cggcgtaaac gatggtcttt
      541 atgttctcca tccaggcgct ggattgccgc ctggaacttg cctttgtagt cgccatgggg
      601 gcaacggact ttcatggtca tgtggcggca tgcgccgggg gtggtcaggt gcgacttgcg
      661 ccggaaatat ggtgacggct gacgccggga ccagtcaggt ggcggcaatg accgtggaag
      721 gcgggcggat gaagcccatg cccccctgtt ctgtcaagca actgcgtcaa gtcgccggga
      781 tcgcggagat gataacgtgg gcatacgcat agggatattc gtcggtcatc gtcagatgga
      841 cctgagccac catcccggcc ggcgttatcg cgcgcagtcg ttccgccgat ccacccgtca
      901 tgagcagcgt cggttggccg cccggcaaat tcaccacacc gagattggaa aaaaacacgc
      961 cacgccggaa gcctgtcccc agcgccttcg cgccggcctc cttggcggcg aaccgcttcg
     1021 cgaacgtgcc agcctggatc ttttccgtcc gccgcatcgc cttggaccgc tcgacgtccg
     1081 tgaacacccg ctccaggaag cgagcgccat gacgctcgat ggctttctcg atccgccgga
     1141 tgtcgcagat gtcggaaccg atgccgagga tcatgccagt ggggctgtgc cagtggggat
     1201 catgccaggg cgatcatact aaaagtggcc gcttcgcgat gccacgattc atccgccggt
     1261 cgcacgctct gcccccttga tcgtcttcag gccgcgcagc ccgacaatca ccttggacaa
     1321 gtgagcgata tcccgcacat cgacatcgac cagcacttcc atgaaatcct gctggcggtt
     1381 gacgatctta aggttggcga tggcgccctc ctgcttggca atcgcgttcg agatgtctgc
     1441 cagcgcgcct tgctcgttgg ccgcgatcgc gctgatacga gccgtgtggc caaagccctt
     1501 cgactctccg ccagccttgc cgaccgcctc ataattccaa tcgacgtcga taaaccgctc
     1561 cggcgtcgct gcaaaggcgg tcagggtttg gcattcgcgg ccgtgaatgg tgacgccctt
     1621 gccggtcgtg acgatgccca cgatttcgtc acccggcacg ggatggcagc agccagcgaa
     1681 cgtataagcc atgcccggga ccaaaccggt gaccggcatg tcgtggtcgt gccgggttgg
     1741 cgttttcccg cgcggcagca tcggcgggac catccgcgac gtgcgcggtg cttgccgcaa
     1801 ctccgggtag gccgcgtgga ccacatcctt agcgatcagg ttgccgttgc cgacggcgac
     1861 atacaaatcg tccaccgtcg ccagtttgag cgccttcagc gccggctcca gcgccttttc
     1921 cgatccgtcc acgccggcct ggcggaacgc cttcgtgagg tcgacgcgcc cggcgtcccg
     1981 agtctgctgg cgctgctcct gatggatgaa gcggcggatg cgggcgcgtg ccttgccggt
     2041 aacgacgaaa cggtcccact gcggtgacgg cgttccgccg cgcgcggtca ttacctcgac
     2101 ctggtcgcca ttttccagat ggtggcgcag cggcatcagg cggccgttta ccttggcgcc
     2161 gacacaggtg tc
//
LOCUS       DASWQN010000937         3512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1540613, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000937 DASWQN010000000
VERSION     DASWQN010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540613"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VGC82_16015"
     CDS             <1..559
                     /locus_tag="VGC82_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY0424825.1"
                     /translation="FVAALSMGWLAKHLDTIGIDQRTNTSATALMITDRGGNIVVRVP
                     DAPEWAGRPVPAWIRPVLGRPDHGVEAVTDPEGHAVLAAFVPAGSSPSGLTVVNAVVV
                     PDLAADIEQATYQDLLVIGGAALAALILAWVAGRRFIRQPTEALLQAARKWREGDLGA
                     RATVIDGGSEFSALGQSFNAMAAGL"
     gene            581..1909
                     /locus_tag="VGC82_16020"
     CDS             581..1909
                     /locus_tag="VGC82_16020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0424826.1"
                     /translation="MQASVLESQVAERTRELSEAANRLQVEIAGRGKTEAALHQAQKL
                     QAVGQLAGGIAHDFNNMLATVLGNLELMERRVAQAGEDWAPADSDRMLRLIERATGAV
                     TRGAQLTSRLLAFSRRKRLEARSTDINALLTELITLANSTIGRRVQVVPELADDLWPA
                     MVDPSQVEAAILNLCLNARDAMPQGGRLTMQTANVTIPAGGGQNAGNKTLASGGYVKI
                     CIADNGVGMSPEVKARAFDPFFTTKSSSGGSGLGLSQVHAMVQQAGGSIWIDSTVGEG
                     TCVTLLLPRAMTASEATAAPTEDPVNLPMHSPAGELVLVVDDDNAVRQVTVEMVRDLG
                     CEVAQASGGEQALALIEKLTPKLILLDYAMPGMNGLQLASALRERGVAAPIALVTGYA
                     DLSEADVSAAKLAGLLRKPFTIRELNVLLMQLRATAGADQALLESGLLER"
     gene            complement(1927..2793)
                     /locus_tag="VGC82_16025"
     CDS             complement(1927..2793)
                     /locus_tag="VGC82_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEY0424827.1"
                     /translation="MEHLSGFAPLAGRYNGFVLDLWGVIHDGINAFPHAVDTLRHLRD
                     AGKRTLLLSNVPRPNDAVQTMMRRMGIEDSLYTGILTSGEAVRRALKDPPDLWWTELG
                     TRVFHLGPDRDKPVLEGLSLTTVASPAEADFVLNTGPDDHRNPSDINEFEPTLAACAR
                     HRLKMICANPDLEVIRGGVRVLCAGALAARYRQLGGDVRSLGKPDPAIYQPVLEQLGL
                     APGEVLAVGDSLRTDIAGAVGVDLAACWVLDGLHGDDLRTSDGRFDTAKAEAAATEAG
                     VVPVATVPRFVW"
     gene            complement(2793..>3512)
                     /gene="pyrD"
                     /locus_tag="VGC82_16030"
     CDS             complement(2793..>3512)
                     /gene="pyrD"
                     /locus_tag="VGC82_16030"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HEY0424828.1"
                     /translation="NNLGLAIYARNLARLSDRGIPLGANVGINKEAADPVRDYPAMIE
                     AIRSLVDYAVINVSSPNTPGLRDLQSEAQLRSILRAVGSVIGRPPILVKIAPDLSHDG
                     LASVVETCAGEGVEGLIVGNTTISRPSGLKSRYASEAGGLSGLPLQRLSTTMLARAYL
                     LARGRLVLIGTGGVFTGRHALAKIQAGASLVQLYSSFALHGPALIPKLKAELLTALRD
                     ARFASVQDAVGTRAQELAEQT"
BASE COUNT          633 a         1082 c         1190 g          607 t
ORIGIN      
        1 attcgttgcc gccctgagta tgggctggct ggcgaagcat cttgatacaa tcggaatcga
       61 ccagcgtacc aacacgtcgg cgacggcgct tatgattacc gatcgtggcg ggaacatcgt
      121 tgtccgggtg cctgatgccc cggaatgggc cgggcgtccg gttcctgcct ggattcgccc
      181 tgtcctcggc cggccggatc atggcgttga agccgtcacc gatccggagg gacacgccgt
      241 gttggcagcc ttcgtgccgg ccgggtcgtc gccgagtgga ctgaccgtgg tcaacgccgt
      301 ggtagtgccg gatctggcgg ccgatatcga acaggctacc tatcaggatc ttctggtgat
      361 tggcggtgcc gcccttgccg ccttgatcct ggcatgggtc gcaggccgtc gcttcatccg
      421 gcagccaacc gaggcactgc tgcaggcagc gcgcaaatgg cgggaaggcg acttgggcgc
      481 gcgtgcaact gtgatcgatg gcggttccga attctcggcg cttggccagt cgttcaatgc
      541 gatggcggcc gggctgtagg cgcgtgagct ggaacgacgt atgcaggcca gcgtgcttga
      601 atcccaggtg gccgaacgca cgcgggaact atccgaagcc gccaaccggc tgcaggtcga
      661 gatcgccgga cgaggaaaaa ccgaggccgc gctgcaccag gcgcagaaac ttcaggccgt
      721 tggtcaactc gcgggcggaa tcgcgcatga cttcaacaac atgctggcaa cggttctcgg
      781 caacctggaa ctaatggagc ggcgggtagc gcaggccggg gaggattggg cgcctgctga
      841 ttcggaccgc atgctgcgcc tgatcgagcg ggcgacgggc gcagtaacac ggggcgccca
      901 gctgacatcc cgcctgctcg ccttctcccg ccgcaagcgc ctcgaagcac ggtcgaccga
      961 tatcaacgcg ttgctgaccg agttgatcac cttggcgaac agtaccattg gacgccgtgt
     1021 gcaggtcgtg ccggaacttg ccgatgacct gtggccggcg atggtggacc ccagccaggt
     1081 tgaagccgcg attctgaacc tgtgcttgaa cgcccgggac gcaatgccgc aaggcggccg
     1141 cctgacgatg caaacggcga acgtgacgat cccggcgggt ggtggccaaa acgctggcaa
     1201 caagaccctc gcgtcgggtg gctatgtgaa gatctgcatc gcggataacg gtgtcggcat
     1261 gtcgccggag gtcaaggcca gggcgttcga tccattcttt acaacgaaaa gttcaagcgg
     1321 cggctcgggt ctgggcttga gtcaggtcca tgccatggtc cagcaagccg gcggcagcat
     1381 ctggatcgat agtaccgtgg gcgaaggaac ctgcgtaacg ctgcttctgc cgcgggcgat
     1441 gacggcgagc gaggcgacgg cggcgccgac ggaggatccg gtcaatcttc cgatgcacag
     1501 ccccgccggc gaactcgtgc tggtcgtcga tgacgacaat gcggtgcggc aggttaccgt
     1561 cgagatggtg cgcgaccttg gttgcgaggt ggcccaggca tccggcggcg agcaggccct
     1621 ggcgctgatc gagaagctga cgccgaagct tattctcctg gactatgcga tgccgggcat
     1681 gaatggtctg cagcttgcca gtgccctgcg ggaacgcggt gtagccgccc caatcgcgct
     1741 ggtcaccgga tatgccgatc tgtcagaggc cgacgtcagc gcggcaaaac tcgcaggatt
     1801 gttgcgtaaa ccgttcacta ttcgagagct gaacgttctg ctgatgcagt tacgcgccac
     1861 cgccggtgcg gaccaggcgc ttttggagtc cggattgctg gagcggtgat agcggcttcg
     1921 gcttgcctac cagacgaaac gcggcacggt cgcgaccggc accacgccgg cctcggtcgc
     1981 cgctgcttca gccttggcgg tgtcgaaacg tccgtcggac gtcctcagat cgtccccgtg
     2041 caagccgtcg agcacccagc acgctgccag gtcgactccc accgcgcccg ctatgtcggt
     2101 gcgcagcgaa tcccccaccg ccagcacctc tccaggagct aagccaagct gttccagcac
     2161 cggttggtag atcgccggat cgggcttgcc gagggagcgg acgtcgccac cgagttgcct
     2221 gtaacgcgcc gcgagggcac ctgcgcacaa tacgcggacg ccgccgcgga tgacctccag
     2281 gtcggggttg gcacagatca tcttcagccg atgtcgtgcg catgctgcca atgtcggctc
     2341 gaactcgttg atgtcgctgg ggttgcgatg gtcgtcgggg ccggtgttca gcacgaaatc
     2401 ggcttcggcc ggactggcca ccgtggtcag ggaaagccct tcgagaaccg gtttgtcacg
     2461 atccgggcca aggtggaata cgcgggttcc gagttcggtc caccagaggt cggggggatc
     2521 cttcagcgcc cgccggactg cctccccgct ggtcaggatg ccggtgtaga gcgagtcttc
     2581 gatccccatg cggcgcatca tcgtttgcac cgcgtcgttg gggcggggca cgttggacag
     2641 cagcagcgtc cgcttgccag catcgcgcag gtgccggagc gtgtccacgg cgtgcgggaa
     2701 cgcattgatc ccgtcgtgaa tcacgcccca aaggtcgaga acgaagccgt tgtagcgccc
     2761 ggcgagcggg gcaaaccccg acaaatgctc catcaagtct gctccgccaa ttcctgtgct
     2821 cgtgtgccga ccgcatcctg aacgctcgcg aaccgtgcgt cacgcagggc cgtcaggagc
     2881 tccgccttca gcttcgggat cagtgccgga ccgtgaagcg cgaagctgga gtaaagctgc
     2941 accagggagg cgccggcctg gatcttcgct aaagcgtggc ggccagtaaa cacgcccccg
     3001 gtgccgatca gcacgaggcg cccccgagcc agcaagtagg cacgcgcgag catggtggtg
     3061 gaaagacgct gaagcggcag gccggacagg cccccggcct cactcgcata ccgagacttc
     3121 aggccgctcg ggcgagagat cgtggtgttg ccgacgatca ggccttcaac gccttccccg
     3181 gcacaggttt caacaacgga ggcgaggccg tcgtgcgaaa ggtcgggcgc gatcttgacc
     3241 aggatcggcg gcctcccgat gacgcttcca acggctcgga gaatagaccg gagctgcgct
     3301 tcggattgca ggtcgcgcaa tccgggcgtg ttcggcgaag acacattgat gacggcgtag
     3361 tccaccaggg agcggatcgc ctcgatcatc gccggataat cgcggacggg atcggcggct
     3421 tccttgttga tgccgacatt ggcgccgagc gggatgccgc gatcggacag acgcgcgaga
     3481 ttgcgagcat aaatggcgag ccccaggttg tt
//
LOCUS       DASWQN010000938         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_617816, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000938 DASWQN010000000
VERSION     DASWQN010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617816"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            494..1672
                     /locus_tag="VGC82_16035"
     CDS             494..1672
                     /locus_tag="VGC82_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEY0424829.1"
                     /translation="MASLPNRLPGLNFDLGEGAEMLRSTVEAFAAAEIAPRAAAIDRD
                     NAFPMDLWPKMGALGVLGVTVSEQYGGAGMGYLEHVVAMEEISRASASVGLSYGAHSN
                     LCVNQIFRNGNDDQKRRYLSGLIDGTRVGALAMSEPGAGSDVVGMRTRAEKKGDRFVL
                     NGSKMWITNGPDADVLVIYAKTDPAAGPRGITAFMVEKGFKGFSTAQKLDKLGMRGSN
                     TCELVFQDCEVPEENVLSTVGRGVNVLMSGLDYERAVLAAGPIGIMQACMDVVVPYVH
                     ERKQFGQAIGEFQLMQGKLADMYTTMNASKAYVYAVAKACDRGETTRKDAAGAILYAA
                     EKATWMALEAIQALGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFAETA
                     "
BASE COUNT          380 a          515 c          718 g          389 t
ORIGIN      
        1 ccgagccagc aacacggcgt cctcaggagt tagccaggac cactcggctc cacgtgcgcg
       61 aaccgcaacc cagcaagtgc ctccgtggac gtgttcttca gtcgctacgg gaccaaagtt
      121 tcgcgtcatg tcatgctcct gttctgtctc acggcgatgc cgaataatgg acgctggtta
      181 gtggttgcag atcgcgatag ctgtgaccgg ggccacaagc tggcgatttc gccttgaacg
      241 caggaggttt gaattggttc aggcatcgcg gatagacgtc gtcatgctcg cgaggccgtg
      301 cagggcttgg cccgcgcgag aggtgcctga aactccagtg tgcttcggct tattttccta
      361 acccatcccg cgtcacccgg caagcagaaa attgtattcc tgcgggaatg gcgacctgat
      421 tagcggcggc ttggcgaagg cggacggcgg gagcagtgtg gccgcgtttt tccaggatca
      481 tcggggaata agaatggcga gcctgccgaa ccgtctgccg ggactgaact tcgatttggg
      541 agagggcgcc gaaatgctgc gctccaccgt cgaagcgttc gcggcggcgg aaatcgcgcc
      601 gcgggcggcg gcgatcgatc gggacaatgc gtttccgatg gatctgtggc cgaagatggg
      661 tgccctgggc gtgctggggg taacggtgtc cgaacagtat ggcggcgccg gcatgggcta
      721 tctggagcat gtggtggcga tggaagagat ttcccgagcc tccgcctcgg tggggctgtc
      781 ctatggggcc cattccaacc tgtgcgtgaa ccagattttt cgtaatggca acgatgacca
      841 gaagcggcgg tacctgtccg ggctgatcga tggaacccgt gtgggcgcat tggcgatgag
      901 tgagccgggg gcgggatcgg acgtggttgg gatgcgcacc cgggcggaaa agaagggtga
      961 ccgcttcgtg ctgaacggca gcaagatgtg gatcaccaat ggcccggacg ccgacgtgct
     1021 ggtgatctat gcgaagaccg accccgcggc ggggcctcgg ggcatcacgg cgttcatggt
     1081 cgagaagggg ttcaagggat tctcgaccgc gcaaaagctg gacaagctgg ggatgcgcgg
     1141 gtcgaacacc tgtgaactgg tattccagga ctgcgaggtt cccgaggaga atgttctttc
     1201 cacggtcggg cgtggggtga atgtgctgat gagcggactg gattatgagc gagcggtgct
     1261 ggcggccggg ccgatcggga tcatgcaggc atgcatggat gtggtggtgc cttatgtgca
     1321 cgaacgcaag cagttcggcc aggcgatcgg cgagtttcaa ttgatgcagg gcaagttggc
     1381 ggacatgtac acgacgatga atgcctccaa ggcctatgtg tatgcggtgg cgaaggcctg
     1441 cgaccgcggc gagacgaccc gcaaggatgc ggcgggggcg atactttacg cggccgagaa
     1501 ggcgacgtgg atggcgctgg aggcgatcca ggcgctgggc ggcaacggct acatcaatga
     1561 ctatccgacg gggcggttgc tgcgggatgc gaagctgtac gaaatcggcg ccgggacaag
     1621 tgagatcagg cggatgctga tcgggcgcga gctgttcgcg gagacagctt gatgatggct
     1681 ttatccggtt attggtgggc gactggggtg caccgtgccg aaccggcaag caagtttttg
     1741 acacggaggc cgcggaggat cacggagcac cacggaaaag taaagcgtgg cgcttcgtgc
     1801 ggtgtatgcg cgggtagggc tcggcttcgc atcgactgct tcaagtggct ggaatgctgt
     1861 cacgctgact ggaaaacatg cgggccggct taccgcctgg cgtgtatgcg tcatggccat
     1921 gcttggcggg gccacccgcg actttccttg cagccacaac aggactctgt tttcagttac
     1981 cgtgaagccg tttcagtcgg tc
//
LOCUS       DASWQN010000939         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_485664, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000939 DASWQN010000000
VERSION     DASWQN010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485664"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VGC82_16040"
     CDS             <1..432
                     /locus_tag="VGC82_16040"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0424830.1"
                     /translation="QSFKSGNSVIGTTWQVIVNTAAAEKAPVKAILPTEGATGWSDTW
                     MVAAKSKHPNCAYQWMNWIVSPKGNAQVAEYFGEAPANEKACAQTSDKSFCETYHATD
                     AAYADQIWYWSTPIPQCLDGRTDVSCTDYSAWTRAWTEIKG"
     gene            439..1317
                     /locus_tag="VGC82_16045"
     CDS             439..1317
                     /locus_tag="VGC82_16045"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0424831.1"
                     /translation="MTVDSARRRLSARLTRSPRGRLALLLSGPLLWLGLAYLGALAAL
                     LVTALWTTNAFTGDIERVWTLDNIRTVLTDSVYRAVTLRTIGVAALVTVIDAVLALPV
                     AFFMAKVAARRWQPLLVAAILLPLWASYLVKAYAWRSMFGGNGLLDWLGSPVGLHSPG
                     YGLPATVCTLASLWFPYMVLPIFAGLERLPNSLLEAAGDLGAQPGRTLRTVVLPLVFP
                     AIVAGSIFTFSLSLGDYVAVKIVGGTKQFLGNLVYDNIGAADNQPLAAAVGLVPVVVM
                     VVYLFLSRRAGALDNL"
     gene            1335..2120
                     /locus_tag="VGC82_16050"
     CDS             1335..2120
                     /locus_tag="VGC82_16050"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0424832.1"
                     /translation="MRITLRIITGVILTVVYVPLVVVVINSFNADRAFGWPPSGFTLT
                     WWSRAMHNGGALDALRTSVVVGLFATAIALVLGTLAAMSLQRYRFFGRDAVSLLVILP
                     IALPGIVTGIALNNAFRTILGVPLSLGTLIVAHATFCIVVVFNNVIARLRRMAGDLEE
                     ASMDLGADSFTTFWRITFPLMRTALLAGGLLAFALSFDEIIVTRFTASQTTTTLPIWI
                     LDNLFRPNQAPVVNVVATVLVFASIIPIAIAQRLSGDPSGGRL"
BASE COUNT          310 a          772 c          760 g          384 t
ORIGIN      
        1 cagtccttca agagcggcaa ctcggtcatc ggcaccacct ggcaggtcat cgtgaacacc
       61 gcggccgccg agaaggcacc ggtcaaggcg atcctgccga ccgagggcgc gaccggctgg
      121 tccgacacgt ggatggtggc cgcgaagagc aagcacccga actgcgcgta ccaatggatg
      181 aattggattg tcagcccgaa gggcaacgcc caggtcgccg agtacttcgg cgaggcgccg
      241 gccaacgaga aggcctgtgc gcaaaccagc gacaagtcgt tctgcgagac ctatcacgcc
      301 accgacgcgg cgtacgcgga tcagatctgg tattggagca cgcccattcc gcagtgcctg
      361 gacggacgca cggacgtctc ctgcaccgat tattcggcct ggacccgggc ctggaccgag
      421 atcaagggct agccgccggt gaccgtggac agcgcccggc gccgcttgtc ggcgcggctc
      481 accaggtcgc cgcgcggccg gctggcgctg ctgctgagcg ggccgctgct ctggctcggg
      541 ctcgcctacc tcggcgcgct ggcggcgctg ctggtcaccg ccctgtggac gacgaacgcg
      601 ttcaccggcg acatcgaacg ggtctggacc ctcgacaaca tccggaccgt gctgaccgac
      661 tcggtctacc gtgcggtcac gctgcgcacc atcggcgtgg ccgcgctggt caccgtgatc
      721 gacgcggtgc tggcgctgcc ggtggcgttc ttcatggcca aggtcgccgc aaggcgctgg
      781 cagccgctgc tggtcgccgc gatcctgctg ccgctctggg cgtcctacct ggtcaaggcg
      841 tacgcgtggc gcagcatgtt cggcggcaac ggcctgctcg actggctggg ctcgccggtc
      901 gggctgcact cgcccggcta cgggctgccg gccacggtgt gcacgctggc gtctctgtgg
      961 ttcccgtaca tggtgttgcc gatcttcgcc ggcctggaac gcctgccgaa ctcgctgctg
     1021 gaggcggccg gcgacctcgg cgcccagccc ggccggacgc tgcgcaccgt ggtgctgccg
     1081 ctggtcttcc cggcaatcgt ggccggttcg atcttcacgt tctcgctctc gctgggcgac
     1141 tacgtggcgg tgaagatcgt cggcggcacc aagcagttcc tcggcaacct ggtctacgac
     1201 aacatcggcg cggccgacaa ccagccgctg gcggcggccg tcggcctggt cccggtggtg
     1261 gtgatggtcg tgtacctgtt cctgtcccgc cgggccggcg cattggacaa cctctgatgt
     1321 acctgacccg gggcatgcgg atcacgctgc ggatcatcac cggcgtgatc ctgaccgtcg
     1381 tgtacgtgcc gctggtcgtg gtggtgatca actcgttcaa cgcggaccgg gcgttcggct
     1441 ggccgccgtc cgggttcacg ctgacctggt ggtcgcgggc gatgcacaac ggcggggcgc
     1501 tggacgcgct gcggacctcg gtcgtggtcg ggctgttcgc caccgcgatc gccctggtgc
     1561 tgggcacgct ggccgcgatg tctttgcagc gctaccggtt cttcggccgc gacgccgtgt
     1621 cgctgctggt gatcctgccg atcgcgctgc ccggcatcgt caccggcatc gcgctgaaca
     1681 acgccttccg cacgattctc ggcgtgccgt tgtcgctggg cacgctgatc gtcgcgcacg
     1741 ccaccttctg catcgtggtg gtgttcaaca acgtgatcgc gcggctgcgc cggatggccg
     1801 gcgacctcga ggaggcgtcg atggacctcg gcgcggacag cttcaccacg ttctggcgga
     1861 tcacgttccc gctgatgcgc accgcgctgc tggccggtgg gctgctggcg ttcgcgctga
     1921 gcttcgacga gatcatcgtg acccggttca ccgccagcca gacgaccacc acgctgccga
     1981 tctggatcct ggacaacctg ttccgcccga accaggcccc ggtcgtcaac gtggtcgcca
     2041 ccgtgctggt attcgcctcg atcatcccga tcgcgatcgc ccagcgcctg tccggcgacc
     2101 cctccggcgg ccgcctctag cccgtgccga gcgtgaacag gaccgcgatg gccagcaaca
     2161 ccgggacgca ggcgatcgtg gacaggaaga tggcgtcgcg ggccacggtc agaccggtct
     2221 ggaaac
//
LOCUS       DASWQN010000940         4192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2070162, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000940 DASWQN010000000
VERSION     DASWQN010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2070162"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VGC82_16055"
     CDS             <1..1386
                     /locus_tag="VGC82_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY0424833.1"
                     /translation="STGKRPAWLPELRSLVALSWPLAVGNLTQIGMGTTDVMMMGSLG
                     PNTLAAGALGTNLYFMTWIFGIGLLNAVAPMTARELGRGSPTIRGVRKIVQHGFLSAG
                     CVAVPCWLILWWSEPLLVAMGEDAALSADAGTYVHMLQWALLPSWCYLVLRSFVSALE
                     RPIWTLYIGAAAVLVNAAANWCLMLGHCGCRSLGIAGSGLATLLSSLLMFAALGIVVC
                     TVRPFRRYRLFAAFWHSDWPLLRALWRLGLPMAATLSFEVTMFNAAVFLMGLIGAASL
                     AAHSIAIQLAGLSFMVPLGIGQAVAVRVGRAYGAQDRKAVGRAGWTAFWLVVAFMATM
                     SLTMILAPRLLISAFLDTADPGNAEVVGLATSFLAFAALFQIADGTQAVGAGMLRGLH
                     DARMPMLFALAGYWGIGLPLGLLLAFPAGLNGIGIWIGLSTGLAVVACLMIRRWLHRE
                     ALGLLLLRPEE"
     gene            1681..2523
                     /locus_tag="VGC82_16060"
     CDS             1681..2523
                     /locus_tag="VGC82_16060"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0424834.1"
                     /translation="MITLPQLRPLGEPLGMEALGVDLSKPLDNETFAWVSDAFAQNPV
                     LVFRDQDLGAAELAAFGRRFGPPRVHALTKYRHAEYPEVSWLTNVEEDGKVDWYGVKR
                     ATDWHTDSTYEDDPPILAILHAKAVPSEKGGTMFADMRAAYDALPAAMKERLQGLQGL
                     HGRSNGPAGERLYGDDKGVTEKQYNELARPAVIQHPVTGRPILFVNPMHTHGFVGMTQ
                     AEAWPLIEELAAHSTQDRFVYYHHWRVGDVLMWDERATMHRGAGDYRPDEHRVMLRTI
                     VYAN"
     gene            complement(2592..3953)
                     /locus_tag="VGC82_16065"
     CDS             complement(2592..3953)
                     /locus_tag="VGC82_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HEY0424835.1"
                     /translation="MSDAALPQPRRRGLQSLAPTFVGLFERYMPDPFVLAIGLTALTA
                     ILALLIAPKGSPAMIATSWYTGIFAILGFAFQMILILATGHALAHAPAVARILRRLVA
                     MAHTPAQAVVLTFLVAAIASLLNWGFGLVVGAILAREVARQVRVDFGWLVAAAYSGFA
                     VWASGISSSIALTQATHGNALNIVEKLTGQLLPLSDTVFATFNWLPTLLIVLVMPMVF
                     IAIRPADTDVVAFVPEPEVPPPPPDVSSSPARRMDRSPVGSLFLLAAGLAAVAVLWLQ
                     GTFTFDVNMVILIFLIGGIALHGTPIAYGEAMKNAARQTGGMMLQYPFYGGIMGIMSG
                     TGLADAISKGFVAVSSAHTLPFWSYICSLIITFFVPSGGGHWAVQGPFVVPAAVTLHA
                     SMPATAMAVAMGEQVANMLQPFWALPVVAMAGIGIQRVMGYTVMTFVVTGIIYALALL
                     LLV"
BASE COUNT          743 a         1349 c         1344 g          756 t
ORIGIN      
        1 tcgaccggca agcgcccggc atggttgccg gaactccgtt ccctggtcgc gctgagctgg
       61 ccgttggcgg tcggcaacct gacacaaatc gggatgggca cgaccgacgt gatgatgatg
      121 ggaagccttg gtcccaatac actcgcggcc ggcgcacttg gcacgaatct ctacttcatg
      181 acgtggatct tcggcatcgg cctgctgaac gccgtcgcgc ccatgaccgc gagggagctt
      241 ggccgtggtt ctccaacgat tcgcggcgtg cgcaagatcg tccagcacgg gtttttgtcg
      301 gcgggctgcg tcgcagtgcc gtgctggctg attttgtggt ggagcgagcc gctactggtc
      361 gcgatgggtg aggatgccgc tttatccgcg gacgccggaa cttatgtcca catgctgcaa
      421 tgggccctgc tgccgtcgtg gtgctatctt gtgctgcgat ccttcgtcag cgcgttggag
      481 cgcccgatct ggacgcttta catcggtgcc gcggcagtcc tggtcaacgc cgcggctaat
      541 tggtgcctga tgctgggaca ttgtggctgc cgatcgcttg gtatcgccgg atcaggcctc
      601 gccacattgc tttccagcct gctgatgttc gccgctctgg gcatcgtcgt gtgcaccgta
      661 aggccgtttc ggcgctaccg gctgttcgcc gcgttctggc attccgactg gccgctgctg
      721 cgggcgctct ggcgcctcgg cctgcccatg gcggcgacgc tcagtttcga agtcacgatg
      781 ttcaatgccg cggtctttct gatggggttg atcggtgcgg cttcgctcgc ggcccattcg
      841 atcgcgatcc aactcgcggg actgagcttc atggtgccgc tcggcatcgg ccaggcggtg
      901 gccgtgcggg tcgggcgggc ttacggtgcg caggacagga aggcggtcgg acgcgccgga
      961 tggacggcgt tttggcttgt cgttgccttc atggccacga tgtcgctgac gatgatcctg
     1021 gcaccgcggt tgttgatttc ggcgtttctc gataccgcgg atccaggcaa tgccgaggtc
     1081 gtcggcctcg cgacatcgtt tctggcattc gcggctttgt tccagatcgc ggatgggacc
     1141 caggctgtcg gcgccggcat gctgcgcggg ttgcacgacg cacgcatgcc gatgctcttt
     1201 gcactggccg gctattgggg cattggcctt ccgcttgggc tgctgctggc ctttcccgca
     1261 ggtctcaacg gcattggaat ctggatcggc ctctcgacag ggttggctgt cgtcgcctgc
     1321 ctcatgatcc ggcggtggct gcaccgtgaa gcgctcggac tgttgctgct ccgaccagaa
     1381 gagtgaccga acggcactgt cctcaaccgg tttggcttcg tccaggcgga ccacgggcgg
     1441 ttggcctgga attgccgcac ccagcccggc tacgagcctc gcggccagac cgggtgctga
     1501 ccagacgggt actgaccaga ctgggtactg accagaccgg gtactgaccg gcctggatgc
     1561 tggccagacc ggatgcggtt gcatcgcctg atcaggcggc tacagtggcc atatgtttca
     1621 ttcggcgata gccccacagg gaccaatcgc cggtttggcg acaaaccagg aggatcgctc
     1681 atgatcacct tgccgcaact ccgtccgctt ggcgaaccgc tcggtatgga agcgctcggc
     1741 gttgacctgt cgaaaccgct cgataacgaa accttcgcct gggtcagcga cgccttcgcg
     1801 cagaatccgg tgctggtgtt tcgcgaccag gatcttggcg ccgccgaact tgccgccttt
     1861 ggccgccgct ttgggccgcc gcgcgttcat gcgctgacga agtaccgcca cgccgaatat
     1921 cccgaggtct cctggctgac caatgtcgag gaggacggca aggtggactg gtatggcgtc
     1981 aagcgcgcca ccgactggca taccgattcg acctatgagg acgatccgcc gatcctggcg
     2041 atcctgcacg cgaaagcggt gccgtcggaa aagggcggca cgatgttcgc cgatatgcga
     2101 gccgcctatg acgcactgcc cgcggcgatg aaggagcgcc tgcagggcct gcaaggtttg
     2161 cacggccgca gcaatggccc agccggcgag cggctgtatg gcgacgacaa aggggtgacc
     2221 gagaagcagt ataacgagct ggcccgcccc gccgtcatcc aacacccggt caccgggcga
     2281 ccgatcctgt tcgtcaaccc gatgcacacg cacggcttcg tcggcatgac ccaggcggag
     2341 gcgtggccgc tgatcgagga actggccgcc cattcgacgc aggaccgctt cgtctattac
     2401 caccattggc gggtcggcga tgtactgatg tgggacgagc gggcgaccat gcaccgtggc
     2461 gccggcgatt accgccccga cgagcaccgc gtcatgctgc gcacgatcgt ctatgcgaac
     2521 tgaatgcgcg gtctgcgcca cgggttttgc ccgttttggc aacccttcag gctgggcgtg
     2581 tcatgctgca ttcaaaccag cagcaacagc gccagcgcat agataattcc tgttacgaca
     2641 aacgtcatga cggtgtagcc catcacgcgc tggatgccga ttccggccat cgccacgacc
     2701 ggtaacgccc agaatggctg cagcatgttg gcgacctgct cgcccatcgc caccgccatg
     2761 gcagtggctg gcattgaagc gtgcaaggtt actgccgccg gcaccacgaa aggcccttgc
     2821 acagcccagt gcccgccccc gctcggcacg aagaacgtga tgatcagcga gcagatatag
     2881 ctccagaacg gcagggtatg cgcactggaa accgcgacaa acccctttga gatcgcatcc
     2941 gccaaaccgg tgcccgacat gatgcccatg atgccgccgt agaacgggta ttgcagcatc
     3001 atgccgccgg tctgtctggc cgcgttcttc atcgcctcgc cataggcgat cggtgtgccg
     3061 tgcagcgcga tgccgccgat gaggaaaatc agaatcacca tgttcacgtc gaaggtgaac
     3121 gtcccctgca gccacagcac ggccaccgcg gccagccccg cggccaacag aaacaggctg
     3181 cccacgggcg acctgtccat gcgccgagcg ggggagctgg acacgtcggg cggcggcggc
     3241 ggcacctcgg gctcgggcac gaatgccaca acgtcggtgt ccgccggccg gatggcgata
     3301 aacaccatcg gcatgaccag cacgatcagc agggtgggaa gccagttgaa cgtcgcgaac
     3361 accgtgtcgg acaggggcag caactggccg gtcagcttct cgacgatgtt caatgcgttg
     3421 ccatgggtcg cctgcgtcaa cgcgatcgag ctggagatgc cgctcgccca caccgcgaag
     3481 ccggagtagg cggcggcgac cagccagccg aaatccaccc ggacctgccg cgccacctcg
     3541 cgggccagaa tggcacccac caccaggccg aagccccagt tcagcaggga tgcgatcgcc
     3601 gccaccagga acgtgagcac caccgcctgg gcaggcgtgt gcgccatcgc cacgagccgg
     3661 cgcaaaatgc gcgcgaccgc cggcgcatga gcgagcgcat gccccgtcgc aaggatcagg
     3721 atcatctgga acgcaaagcc caggatcgcg aatatgccgg tgtaccacga cgtagcgatc
     3781 atggccggcg agccctttgg tgcgatcagc agggccagga ttgccgtgag ggcggtcagg
     3841 ccgatcgcca gcacgaacgg atcgggcatg tagcgctcga acaagccaac aaatgtcggc
     3901 gcgagcgatt gcagtccacg acggcggggc tgaggcagag cagcgtcgct catttttctt
     3961 gggatccttg cttcctctcg acagaagcaa agaacccaat ccaatgaagc tggttccgtc
     4021 cccgcggacc caaacccgac tccgccgaga tcggctactc cgccgccaag cgcggtgaaa
     4081 acgctgaaac ctccgcgcgt tcggcactcg ccatcggctg cggccaccag ggatcagccc
     4141 actccgcgaa cagtggctgc agcttcggca tcacctgttc agcgaacagc tt
//
LOCUS       DASWQN010000941         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_90652, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000941 DASWQN010000000
VERSION     DASWQN010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90652"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VGC82_16070"
     CDS             <1..440
                     /locus_tag="VGC82_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0424836.1"
                     /translation="SLIRDIAGQINLLALNATIESARAGDAGKGFAVVASEVKNLARQ
                     AGNATDQIAREIDSLQVVSAEVVSALGTITKSVDSVMEYVTVTASAVEEQSVVTQEMS
                     EAMQKTAGNVVAVNDNMVEIIAAVQQVGQAVNNTRKAAQVLVR"
     gene            576..827
                     /locus_tag="VGC82_16075"
     CDS             576..827
                     /locus_tag="VGC82_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY0424837.1"
                     /translation="MTETLLDVKGMNCPLPVLKANRRLRGMAAGERLRVLATDRAAVA
                     DFHAFCRETGHALLAWSEEAGVFSFVIRRRADETEKKGS"
     gene            829..1761
                     /locus_tag="VGC82_16080"
     CDS             829..1761
                     /locus_tag="VGC82_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY0424838.1"
                     /translation="MTTALITHKACLEHDTGPYHPETSERLRAVLAALEAPEFSDLLR
                     ESAPLATVEQLSRVHPRAYVEAILSIRPEPGEPVQLDGDTVMSHGSAEAATRAAGAAC
                     MAVDAVMEGWARAAFAAVRPPGHHAEPERPMGFCLFNNAAVAALHARARWGIQRVAVV
                     DFDVHHGNGTQAMFAADPDLFYASSHQSPCYPGTGEPWERGAADNIVNAPLRPRDGSA
                     AFRAAWSDIILPALRAFAPGLLIVSAGFDAHVADPLAQLRLETADFAWITEQLVAVAE
                     EHCGGRLVSVLEGGYDLDALAASAAVHVRGLMRI"
     gene            1898..>2247
                     /locus_tag="VGC82_16085"
     CDS             1898..>2247
                     /locus_tag="VGC82_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0424839.1"
                     /translation="MRFGLFGAAQAQRGGSPDVDSAAGFNDYIDYLIEAEALGYHSSF
                     IVEHHFTGFGQVSATLNLLSWIGAKTTTLRLGTAVIVLPWHNPVLLAEQAATVDLLSG
                     GRLDFGIGKGYRHN"
BASE COUNT          403 a          709 c          734 g          401 t
ORIGIN      
        1 tcagcctgat ccgtgacatc gccgggcaga tcaatctgtt ggcgctgaac gcgacgatcg
       61 aatcggcgcg cgccggtgat gcgggaaaag gctttgcggt ggtggctagc gaggtaaaga
      121 acctggcacg ccaggcgggc aacgccaccg atcagatcgc acgcgagatc gacagcttgc
      181 aggtcgtgtc ggcagaagtc gtgtccgcgc tgggcacgat caccaagtcg gtcgactcgg
      241 tgatggaata cgtcactgtg accgcgagcg ccgttgagga gcaaagtgtc gtgacccagg
      301 aaatgtcgga ggcgatgcaa aaaaccgcgg gcaatgtggt tgccgtaaat gacaacatgg
      361 tggaaatcat agcggcggtg cagcaagtag gacaggcggt gaacaatacg cggaaagccg
      421 cccaggtcct cgtcaggtga ggcctgatgc gcgtgcatgt gccttgatcg tccgagaact
      481 gaacgccggg accgccgtca ggcgactccg gtagcggcca cgacttctgc ttgccatcac
      541 ttgcccggcc gccccgtcga acgcatcttg gttgcatgac agagaccctg ctcgacgtga
      601 aaggcatgaa ctgtcccctg ccggtgctga aggccaatcg ccggctgcgt ggcatggcag
      661 cgggcgagcg gcttcgggtg ttggcaaccg atcgcgccgc ggttgccgat tttcatgcgt
      721 tttgccgcga aaccggtcac gccctgctgg catggagcga ggaagctggc gtattcagct
      781 tcgtgatccg ccgccgtgcg gatgaaaccg agaagaaggg tagctgacgt gacgaccgcg
      841 ttgatcaccc acaaggcgtg cctggagcac gataccgggc cataccatcc tgaaacttcc
      901 gaacggctgc gcgcggtgct ggctgcgctg gaggcgccgg aattttccga cctgctacgc
      961 gagtccgctc cgctggccac ggtcgaacaa ctcagccggg tgcatcctcg ggcctatgtg
     1021 gaagcgatcc tgtcgatccg cccggagccg ggcgagccgg tgcaactcga tggcgatacc
     1081 gtcatgagcc acggaagtgc cgaagccgcc acccgcgccg ccggcgccgc ctgcatggcc
     1141 gtcgatgcgg tcatggaggg ttgggcacga gcggcgttcg ctgccgtccg gccgccgggc
     1201 catcacgccg aacccgaacg gccgatgggt ttctgcctgt tcaacaacgc ggccgtggct
     1261 gcgttgcacg cgcgcgcgcg ctgggggatc caacgggtcg ccgtcgtcga tttcgacgtc
     1321 caccacggta acggcacaca ggcgatgttc gccgctgatc cagatttgtt ctacgcttcc
     1381 tctcaccaaa gcccctgcta ccccggcacc ggcgagccgt gggagcgtgg cgccgcggac
     1441 aacatcgtca acgcgccgct tcgcccgcgt gatggcagcg ccgccttccg ggcagcctgg
     1501 tcggatatca tccttcctgc gctgcgtgca ttcgccccag gattgctgat cgtttcagct
     1561 ggcttcgacg cccacgtcgc tgacccgctg gcccaacttc gcctggaaac ggccgacttt
     1621 gcctggatta ccgaacaact tgtcgccgtc gcggaagaac actgcggcgg ccggttggtc
     1681 tcggtcctcg aggggggata tgatctggac gccttggccg cctcggctgc cgtgcatgtc
     1741 cgcgggctta tgcggatctg aggcccatcg accagccgcc gtacgcgact ggggcgttcc
     1801 gcgcgtcaaa gccagtctgg cggcgcatca gacccagtct ggacatttct taccccccgg
     1861 cacaagcttg cctgacgctg caggagcaag agggaccatg cgtttcggac tttttggcgc
     1921 cgcgcaggcg caacgcggag gttccccgga cgtcgatagc gccgccgggt tcaacgacta
     1981 catcgactac ctgatcgaag ccgaggctct aggctaccat agttcgttca tcgtcgagca
     2041 tcatttcacc ggcttcggcc aggtctccgc aacattgaac ctgctgagtt ggatcggcgc
     2101 caagaccacg acgctgcggc tggggacagc ggtgatcgtg ctgccctggc acaacccggt
     2161 gctgctggcg gaacaggcgg cgacggtgga tttgctgtcc ggaggacggc tcgatttcgg
     2221 catcggcaaa ggctaccgcc acaatga
//
LOCUS       DASWQN010000942         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1806150, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000942 DASWQN010000000
VERSION     DASWQN010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806150"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VGC82_16090"
     CDS             complement(<1..264)
                     /locus_tag="VGC82_16090"
                     /EC_number="4.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622937.1"
                     /note="With PurE catalyzes the conversion of
                     aminoimidazole ribonucleotide to carboxyaminoimidazole
                     ribonucleotide in the de novo purine nucleotide
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY0424840.1"
                     /translation="MTTPFPLPPNATIGLVGGGQLGRMSALAAARLGYRCHILTREAD
                     SPAAQVSHAVTISDYSDPVSLRSFAAAVDVISFEFENVSAEGLD"
     gene            complement(261..776)
                     /gene="purE"
                     /locus_tag="VGC82_16095"
     CDS             complement(261..776)
                     /gene="purE"
                     /locus_tag="VGC82_16095"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019084793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY0424841.1"
                     /translation="MTAPIQAAPLVGLIMGSQSDWPTLSHAAGILDRLAVPHEVRIVS
                     AHRTPDRLRSYATTARDRGLKVIIAGAGGAAHLPGMCASWTPLPVLGVPIESHALKGM
                     DSLLSIVQMPAGIPVGTMAIGRAGAVNAALLAAAMLATQDPALAARLDALRAEQTDAV
                     PEAPRQLRGDP"
     gene            complement(778..981)
                     /locus_tag="VGC82_16100"
     CDS             complement(778..981)
                     /locus_tag="VGC82_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEY0424842.1"
                     /translation="MLSDRDSLLRKLHGLRSEHRDLDTVIARLADQGASDQLHLQRLK
                     KRKLMLRDEIAWLESRLIPDSIA"
     gene            1132..1305
                     /locus_tag="VGC82_16105"
     CDS             1132..1305
                     /locus_tag="VGC82_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEY0424843.1"
                     /translation="MSLNTRLDALKERHASLEERIMDEDQRPRPDSDTLTRLKLEKLH
                     LKEEMERLRALVN"
     gene            complement(1338..1916)
                     /locus_tag="VGC82_16110"
     CDS             complement(1338..1916)
                     /locus_tag="VGC82_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HEY0424844.1"
                     /translation="MTLPLMPKATAVWLIEKTSLTFTQIADFCGMHPLEVQAIADGEV
                     AQGIVGYDPVANAQTTLANIRQCEADPNARLKLIVNALPMPKRLRGSRYTPVAKRNDR
                     PDAIAFLLRNFPQLSEAQVGKLLGTTKDTIQKVRERTHWNSANIKPRDPVILGLCSQT
                     DLNAAVSQANDRLAREGRAVPVPPPPEADEAA"
     gene            2106..2297
                     /locus_tag="VGC82_16115"
     CDS             2106..2297
                     /locus_tag="VGC82_16115"
                     /inference="COORDINATES: protein motif:HMM:NF018413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEY0424845.1"
                     /translation="MAQEEDAPPLKRRRLEPLLLDSLGIAELRLYIDELKSEIARVEA
                     DIGRKNSHRSAADAFFKRP"
     gene            complement(2407..2880)
                     /locus_tag="VGC82_16120"
     CDS             complement(2407..2880)
                     /locus_tag="VGC82_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEY0424846.1"
                     /translation="MTTVDFAPLFRTAIGFDRLARLVDTAASSQDAQSYPPYNIEKTG
                     DDSYRLTMAVAGFRPDDLELTVKDNTLVVAGRVANEGPKAEILYRGIAGRAFERRFVL
                     ADHIVVEGADVRHGLLHVGLKRVVPEALKPRKIVIGGAAPVALANDTQDAAAKAA"
BASE COUNT          571 a          928 c          902 g          511 t
ORIGIN      
        1 gtccagccct tcggcgctca cgttctcgaa ctcgaagctg atcacgtcaa cggcggcggc
       61 gaaggagcga agcgataccg gatcgctgta gtcgctgatg gtgacggcgt gggaaacctg
      121 cgccgccggg ctgtcggcct cccgcgtcag gatgtggcag cggtagccca ggcgggcggc
      181 ggccagcgcc gacatgcgtc caagctgccc accgcccacc agcccgatcg tcgcgttggg
      241 cggcagcgga aatggcgtcg tcatggatcg ccccgaagtt gccgaggggc ctcgggcacc
      301 gcgtcggtct gttccgcgcg caacgcatcc agccgagccg ccagagcagg gtcttgcgtc
      361 gccagcatcg ccgctgccag caacgccgcg ttcaccgccc cggcgcgtcc gatcgccatt
      421 gtgcccacgg gaatccccgc gggcatctgg acgatcgaca acaggctgtc catgcccttc
      481 agcgcatgcg attcgatcgg cacccccagc accggcaacg gcgtccagga cgcgcacatc
      541 ccgggcagat gggccgcacc gcccgccccg gcgatgataa ccttcaatcc gcgatcccgg
      601 gcggtcgtgg cgtaggatcg cagccggtcg ggcgtgcggt gggcggaaac gatccgcact
      661 tcgtgcggca ccgcgaggcg gtccaggatc ccggcggcat gactgagcgt tggccagtcc
      721 gactggctgc ccatgatcag tccgaccagc ggtgccgcct ggatgggtgc cgtcatgtca
      781 ggcgatgctg tccgggatca gccggctttc cagccaggcg atctcatcgc gcaacatcaa
      841 tttgcgtttt ttgagccgct gcaggtgcag ttggtcggat gcgccctggt ccgccaggcg
      901 cgcgatcacc gtgtccaggt cacgatgttc gctccgcagc ccgtgcagtt tacgcagcag
      961 cgagtccctg tcgctcagca tagatagcaa cccacggtag acgtgaagga cggtaacacg
     1021 cgtagcggca taagactggc cagacccaag ccaaacgcgt tacgttgcct gtgcccggtc
     1081 gccgtggcgg ccagtggcag ccaaccggtt aaacacggag gtacccagtc catgagcctg
     1141 aatacccgtc tcgacgctct aaaggaacgg catgcatctc tggaggaacg aattatggac
     1201 gaggaccagc gtccgcgtcc agactcagat accctcacgc gactcaagct ggagaagctg
     1261 catctgaagg aagagatgga aaggctacga gcgctggtta actgagcgaa cacagctcaa
     1321 cctacccgtc acgccggtca ggcggcctcg tccgcctccg gcggtggcgg caccggaacg
     1381 gctcgacctt cccgagccag ccggtcattt gcctgcgaaa ccgcggcgtt caaatcggtc
     1441 tggctgcaca agcccaggat caccgggtcc cgcggcttga tgttcgcgct gttccagtgc
     1501 gtccgttcac gtactttctg gatcgtgtcc ttggtggtgc ccagcagctt gccgacctgc
     1561 gcttcggata actgaggaaa gttccgcagc agaaaggcga tagcgtccgg acggtcgttg
     1621 cgcttcgcta cgggcgtata gcgtgaccca cgcaggcgct tcggcatcgg cagcgcgttc
     1681 acgatcaact tcaaacgagc attcggatcg gcttcacact gccggatgtt ggccaacgtg
     1741 gtctgggcgt tggcgaccgg atcgtatccg acgatcccct gcgcgacttc gccgtcggcg
     1801 atggcttgga cttccagcgg atgcataccg caaaaatccg cgatctgcgt gaaggtcagc
     1861 gaggttttct caatcagcca cacggcggtg gctttgggca tcagcgggag ggtcatggct
     1921 ttcacactct caaggtctac cggcggttgg tttcattctg acaatatcgg ttgccggcca
     1981 accagcacat ttgacgataa tagccgccct ggcgcgtcga tatgacaagc caaggggggt
     2041 gggtcaagcg ccatcgaggc gactatgctc ttctggttgt gcatggctga gacagggaga
     2101 tcgagatggc tcaggaagaa gacgccccac cgctgaaacg tcgccggctg gaaccacttc
     2161 ttttggacag cctggggatc gccgaacttc gcctctacat cgacgaactg aagagcgaaa
     2221 tcgcgcgggt agaggccgat atcggccgca agaacagcca ccgcagcgcc gccgacgcct
     2281 ttttcaaacg accctgatgg ctttcaagcg acgctgatgg cttccaagcg acgctgacgg
     2341 ccaccagaaa tagaaacggg ggccggtcgc ccggcccccg caaatcataa acgataagat
     2401 cgtttatcaa gcagccttcg cggcggcatc ctgcgtgtca ttggcgagag caaccggcgc
     2461 cgcaccacca atcacgatct tgcggggctt cagggcctcc ggcacaacgc gcttcaggcc
     2521 aacatgcagc aacccgtgcc gtacgtccgc gccttcgacg acgatgtggt cggcaagaac
     2581 gaagcggcgc tcgaacgcgc ggccggcaat gccgcggtac aggatttctg ccttcggacc
     2641 ctcgttggcg actcgtccgg ccaccaccaa ggtgttgtcc ttcacggtca gctcgaggtc
     2701 gtcaggacgg aaaccagcaa ccgccatcgt caagcgatag ctgtcgtcac ccgtcttttc
     2761 aatgttgtag ggaggataac tctgcgcatc ctggctggat gcggcggtgt ccacgagccg
     2821 cgccaaacga tcgaaaccga tggccgtacg gaacaaagga gcaaaatcaa cggtcgtcat
     2881 aaaagaacct cctgcgaagc aaggtcgttg tt
//
LOCUS       DASWQN010000943         1842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1410582, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000943 DASWQN010000000
VERSION     DASWQN010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410582"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /gene="speD"
                     /locus_tag="VGC82_16125"
     CDS             complement(<1..431)
                     /gene="speD"
                     /locus_tag="VGC82_16125"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEY0424847.1"
                     /translation="MNALSPLGMVSELPSSNQMQSDAERTLPNGSGPNHPDAEVQKDY
                     FVEKDGVKFAGMHLLVDLWGASNLCDPDHIDRALREAAEAAGATILHSHFHHFSPNGG
                     VSGVLVLAESHISIHTWPERDFAAIDIFMCGACNPHDSVPA"
     gene            complement(616..>1842)
                     /locus_tag="VGC82_16130"
     CDS             complement(616..>1842)
                     /locus_tag="VGC82_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424848.1"
                     /translation="LPLIAETMVQGGEIASASRLLEQRKDDPRLAYARALMHQADGDT
                     AQALKLLDVTAAGHDQFDRARAAIHAVELRLAAREIDKTKAADALDKLLYAWRGDARE
                     LALRQRVADLRGQTGAWRVALSTLRQAEIDFPEQAAPLRERLKASFASMIRDQGAHPT
                     SPIDFVAMVGENTDLVPDSADDEAVEQGLAERLLALDLPTRAKPVLEKLLRQAKSDAA
                     RGNFGLALATLESQEGNDSGAIAVLDSSQGRDLSADVAEQRLILRAVSVARQGNPAAA
                     AAMLAPANTVRAIEARAQIQEGALDWAGAAQAWTDSAALTLPETGMLQEAQMRTVLRL
                     ATATARAGDDVGLAALRGKYDQRMGAGPLAGMFRLLTAEPVRTIADIKRSQREMSLAA
                     SLPSNLKALQTGSVMR"
BASE COUNT          329 a          596 c          574 g          343 t
ORIGIN      
        1 aatgcgggaa cgctgtcgtg cggattgcat gcgccacaca tgaaaatgtc gatcgcggcg
       61 aaatcccgtt ccggccaagt gtgaatggag atgtggcttt ccgccagcac cagcacgccg
      121 ctgacacctc cgttcggcga gaaatggtgg aaatggctat gcaggatggt ggcaccggcc
      181 gcttccgccg cttcccgcag tgcgcggtcg atgtgatccg gatcgcaaag attggaggcg
      241 ccccaaagat cgaccagcaa atgcatgccg gcgaacttca cgccatcctt ctcgacgaaa
      301 tagtccttct ggacttctgc atcgggatga tttgggcccg aaccattcgg gagtgtccgc
      361 tcagcgtcgg actgcatctg gttgctgctc ggaagttccg agaccatccc cagtgggctg
      421 agtgcgttca tcgcgtaccc cttcctacca gtagacggcc tgttggattg tacgcgcgaa
      481 aacgcgccca ccgtcccctg gggccaagaa aacggaacat gacgatctga tagcggcgat
      541 gcaagccttc tttcgcctct tgccgcgata aatcccacat tacgcggcat ccacagggcg
      601 ccggggcacg gccgatcagc gcataaccga accggtttgc agggccttca ggttgctcgg
      661 cagggatgcc gccaggctca tttcccgctg cgagcgtttg atgtcggcaa tcgtccgaac
      721 cggttcggcg gtcagcaagc gaaacatgcc ggcgagcggc ccggcgccca tccgctggtc
      781 gtatttcccg cgcagcgcgg ctagtccaac gtcgtcgccg gcccgtgcgg tcgccgtggc
      841 caggcggagc acggttcgca tctgggcttc ttgcagcatg cccgtttccg gcagcgtcag
      901 cgcggcggaa tccgtccagg cctgagctgc accggcccag tccaatgccc cttcctgaat
      961 ttgggcacgc gcctcgatgg cccgaacagt attggcgggt gccagcatcg cagcggcagc
     1021 ggccggattc ccctggcgcg caaccgatac ggctcgcagg atcaaacgtt gttcggctac
     1081 gtcggcggac aagtcccggc cttgggaaga gtccaacacg gcaatcgcgc cgctatcgtt
     1141 gccctcttgc gattccaacg tagccagggc gaggccgaag ttgccccttg ccgcatcaga
     1201 ctttgcctgg cgcagcaatt tctccaggac aggtttcgct cgggtgggca gatccagagc
     1261 aagaagccgt tccgccaggc cctgttccac cgcttcatca tctgcggaat cgggcacgag
     1321 atcggtgttc tcgccgacca tagcgacgaa atcgatgggc gaagtcgggt gtgcaccctg
     1381 atcgcggatc atgctggcga aactggcctt caaccgctcc cgaagcggcg cggcctgttc
     1441 ggggaaatcg atttcggcct gccgaagcgt cgaaagcgcc acgcgccacg cgccggtctg
     1501 cccgcgcagg tcggcgacac gttgccgcaa ggccagttcc cgcgcgtcgc cgcgccaagc
     1561 gtaaagcagt ttatccaaag cgtcggccgc cttggtcttg tcgatttcgc gcgctgccaa
     1621 acgcagttcc acagcatgaa tcgccgcgcg tgcccgatcg aactgatcgt gcccggcggc
     1681 ggtcacgtcg agcagcttca gcgcctgggc ggtgtcgccg tcggcctggt gcatgagtgc
     1741 ccgcgcatag gccaggcggg gatcgtcttt gcgttgctcc agcagtcggg aagcggaggc
     1801 aatttcaccg ccttggacca tcgtctcggc gatcagcggc ag
//
LOCUS       DASWQN010000944         3713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2598788, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000944 DASWQN010000000
VERSION     DASWQN010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2598788"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VGC82_16135"
     CDS             <1..793
                     /locus_tag="VGC82_16135"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY0424849.1"
                     /translation="KASIMRGLEPNGTAILPADSDQFARLRDVVGDAAVLTFGVSPNA
                     DVRLVHREADAKGSVIHVDILGQTVSLHLNAAGQHMAMNALATLAVVAALGLDPARAI
                     DGLEAFVPLIGRGARREVALADGSALLLDESYNGNGASMRAALGVLRLQPARRRIAVL
                     GDMLEHGREGPMEHAALADAVAGSVDCLFTCGPLMRNLFDLVPPGLRGSHEADSAALA
                     PVVAAAIRQGDAVLVKGSLGSGMKRVIEAIEAEASRQATVTAGAA"
     gene            793..1881
                     /gene="mraY"
                     /locus_tag="VGC82_16140"
     CDS             793..1881
                     /gene="mraY"
                     /locus_tag="VGC82_16140"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY0424850.1"
                     /translation="MLYNVLRPLADQFILFNLFRYITFRAGGACMTALIVSFLLGPSV
                     IRWLKSVQKQGQPIRLDGPARHLVEKKGTPTMGGLLILGSLTISTLLWADLRNGYVWA
                     VLFVTLGYGALGFWDDYLKVTKRNTRGIPGRLKLLVQAVIGLAAAVWITSLARGPLGT
                     SLTFPVFKEAILPLGYFFPAFGTLVMMGASNAVNLTDGLDGLAIVPTMIAATVFTLIA
                     YLVGNRIFADYLQLNPVVGAGELAVFCSALVGAGMGFLWFNAPPAAVFMGDIGSLALG
                     GALGAVAMVTKHEIVLMIVGGLFVVETLSVIIQVFWYKRTGNRVFLMAPLHHHFEKKG
                     WAEATIVIRFWIISMILALVGLATLKIR"
     gene            1878..3248
                     /gene="murD"
                     /locus_tag="VGC82_16145"
     CDS             1878..3248
                     /gene="murD"
                     /locus_tag="VGC82_16145"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY0424851.1"
                     /translation="MSTFSPAVFSGKRFAVVGLGKNGLPAAIGLAAMGADVVAWDDDP
                     AARTAALDAIAGADDRAAALASPQGLQVRDPAEGPFDFDGLVLSPGIPHRLPKPHRVA
                     QRAIAAGVPIVSDVELLFQAVRASGSRARFIGITGTNGKSTTTALLAHVLEQAGVAVA
                     AGANLGPAALSLPLLPHDGVYVLEMSSYMLERLVSMRFDVGIMLNLSSDHIDRHGDMA
                     GYAMAKRAVFDRQTVRDLAVIGTDDSWSRDMAAWLVQRPARVVCISSAETPLAAGPAL
                     PGSHNAQNSAAATAAARFLGISDRAIAAGLLSYPGLPHRQQRIVTIDGVTFINDSKAT
                     NADASERALVCYDRVVWIAGGMAKEGGIEPLVPLFPRIARAFLIGRDAAEFGATLSRH
                     NVRFDIAGTLEAAVPAAFAAAKAAGAPIVLLSPACASWDQFTGYDQRGDRFAELARNL
                     QAGGAA"
     gene            3245..>3713
                     /locus_tag="VGC82_16150"
     CDS             3245..>3713
                     /locus_tag="VGC82_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY0424852.1"
                     /translation="MTVLSRTDNSLLARWWWSVDHWTLGAIGTLIGFGYIMMLAASPA
                     VAERIGSSRESFILKQVVFLALAGLIVVLVSMLSPRDIKRLAIVGCLLALALTAATMM
                     VGMEIKGARRWISLPGMTIQPSEFLKPCFAVTAAWLLSEGKKSPRFPGLLLAIG"
BASE COUNT          557 a         1158 c         1257 g          741 t
ORIGIN      
        1 gaaagccagc atcatgcgcg gcctggagcc gaacgggacc gccatcctgc cggcagactc
       61 ggaccagttc gcgcgcctgc gcgacgttgt cggcgatgct gccgttctga cattcggcgt
      121 ttccccgaac gccgacgtgc gattggtgca tcgggaggcg gatgccaaag gctccgtcat
      181 tcacgtcgat atccttggcc agacggtctc gctgcacctc aacgcggcgg ggcagcacat
      241 ggcgatgaac gccttggcga ccttggccgt tgtcgcggca ctcggcctcg acccggcgag
      301 ggcgatcgat gggctggagg cgttcgttcc cctcatcggg cgcggcgcgc ggcgcgaggt
      361 cgcattggcg gacggcagcg ccttgctgct ggatgaaagc tacaacggca atggcgcctc
      421 gatgcgggcc gcgctgggtg tgctgcggct gcagccggcc cggcggcgca tcgccgtttt
      481 gggcgacatg ctggaacatg gccgcgaggg cccgatggag cacgcggccc tggcggacgc
      541 ggttgcaggt tcggtcgatt gcctgttcac ctgcggtccg ctgatgcgga atttgttcga
      601 tctggttcca cccggcttgc gtggctcgca tgaggcggat tctgccgcac ttgcgccggt
      661 cgtcgccgcc gcgatccgcc agggcgatgc ggtgctggta aaaggcagcc tgggcagcgg
      721 catgaagcgc gtgatcgagg cgatcgaggc ggaggcttcg cggcaagcca ccgtcacggc
      781 cggagcggcc tgatgctgta caatgttctc cggccccttg cggaccagtt catccttttc
      841 aacctgttcc gctacatcac gttccgcgcc ggtggtgcct gcatgaccgc gctgatcgtc
      901 agcttcctgt tgggaccgtc ggtcattcgc tggctcaaat cggtgcagaa acaagggcag
      961 cctatccgtc tggatggtcc ggcacgtcat ctggtggaaa agaagggcac gccgacgatg
     1021 ggtgggctgc tgattctcgg cagcctgacg atttcgacct tgctgtgggc cgacttgcga
     1081 aacggttacg tctgggcggt gctgttcgtc acgctgggct atggcgcgct gggcttctgg
     1141 gacgactatt tgaaggtcac caagcgcaac acgcgcggga tccccgggcg gctgaaactg
     1201 cttgttcagg cggtgatcgg gctggccgcc gcagtctgga tcacatcgtt ggcgcgcggc
     1261 ccgcttggca cgtccctgac gtttccggtg ttcaaggagg ccatcctgcc gttgggctac
     1321 ttctttccgg ccttcggcac cctggtgatg atgggcgcat ccaacgcggt caacctgacc
     1381 gacggtttgg acgggttggc gatcgtgccg acgatgatcg ccgcgaccgt gttcacgctg
     1441 atcgcatacc tggtcggcaa ccgtatcttc gcggactacc tgcaactgaa ccccgttgtc
     1501 ggggccggtg aactggcggt gttctgctcc gccttggtcg gtgccggcat gggattcctg
     1561 tggttcaacg ctccgcccgc cgcggtgttc atgggcgaca tcggcagcct cgcattgggt
     1621 ggcgcgctcg gtgcggtggc aatggtgacg aagcatgaaa tcgtattgat gatcgtcggc
     1681 ggcctgttcg tggttgagac tttatctgtc attattcagg tcttctggta caagcgcacc
     1741 ggcaaccgag tttttctgat ggcaccactg caccaccact tcgaaaagaa gggctgggcg
     1801 gaggcgacca ttgttattcg tttctggatt atttccatga ttctggcgtt ggtcggtctg
     1861 gcgacgctga aaatccgatg agcacgttca gtcccgccgt gtttagcggc aagcgcttcg
     1921 ccgtcgtcgg cctcggcaag aacggcttgc cggcagcgat tggcctggcc gcgatgggcg
     1981 cggacgtggt cgcctgggac gacgatccag cggcgcgcac cgcagcgctc gatgccatcg
     2041 ccggggcgga cgatcgtgcg gcggcacttg cttcgcccca aggccttcag gtgcgcgacc
     2101 cggcggaggg cccgttcgat ttcgatggac tggtgctatc gccgggaatt ccgcatcgcc
     2161 tgccgaaacc gcacagggtg gcgcaacggg cgatcgcggc aggcgtgccg atcgtctccg
     2221 atgtcgagct gctgttccag gcggtgcggg catccggctc gcgtgcgcgg ttcatcggca
     2281 taaccggcac caacggcaag tccacgacca ccgcgctgct tgcccatgtc ctggaacagg
     2341 ccggcgttgc cgttgccgcc ggcgccaatt tggggccggc cgcgctgtcg ctgccgctgc
     2401 tgccacatga tggagtgtac gtgctcgaga tgtcctccta tatgttggag cgactcgtga
     2461 gcatgcggtt tgatgtcgga ataatgttga acctcagttc agaccacatc gatcgtcacg
     2521 gcgacatggc cggatatgcc atggcgaagc gcgcggtgtt cgaccgccag acggtgcggg
     2581 atctggccgt gatcggaacc gacgattcgt ggtcacgcga catggccgcc tggcttgttc
     2641 aacgtcccgc tcgcgtcgtc tgcatttctt cggctgaaac cccgctggca gcgggtccgg
     2701 ctctgccggg ttcccacaat gcacagaatt cggcagcggc gaccgcggcg gcccggttcc
     2761 tgggtatttc cgatcgggcc atcgcggcgg gtctgctcag ctatcccgga ctgccgcatc
     2821 gccagcaacg catcgtcacc atcgacgggg tgacgttcat caacgacagc aaggcgacca
     2881 acgccgacgc gtcggagcgg gccctggttt gctacgaccg cgtggtgtgg atcgccggcg
     2941 gcatggcaaa ggaaggtggg atcgagccgc tggtgccgct gtttccgcgc attgcccgcg
     3001 cgttcctgat cggccgggac gccgccgagt tcggcgccac gctgtcccgg cacaacgtcc
     3061 ggttcgacat cgccggaacg ctggaggccg ccgtgccggc cgctttcgcc gccgccaagg
     3121 cagcgggagc gccgatcgtg ctgctgtcgc cggcgtgtgc gagttgggat cagttcaccg
     3181 gctacgacca gcgcggcgac cggttcgccg aactcgctcg aaacctgcaa gccggaggcg
     3241 ccgcatgacg gttttgtcgc gcaccgacaa ctcgctgctg gcccgctggt ggtggagcgt
     3301 ggaccactgg accctggggg cgatcggcac gctgatcggc ttcggctaca tcatgatgct
     3361 ggccgccagc cccgccgtcg ccgaacgcat cggatcctcc agggaaagct tcatcctgaa
     3421 gcaggtcgtg ttcctggcgc tggcgggcct gatcgtggtc ctggtttcca tgctgtcccc
     3481 gcgcgacatc aagcgtctcg cgatcgtggg atgtctcctt gcgctggcgc tgactgccgc
     3541 aacgatgatg gtcggtatgg agatcaaggg cgcccgccga tggatttcct tacctgggat
     3601 gacgatccag ccaagtgaat ttctgaagcc gtgctttgcg gtcacggctg cctggctgtt
     3661 gagcgagggc aagaagtcgc cccgcttccc cggcttgctg ctggccatcg gca
//
LOCUS       DASWQN010000945         3437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_882873, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000945 DASWQN010000000
VERSION     DASWQN010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882873"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(622..1014)
                     /locus_tag="VGC82_16155"
     CDS             complement(622..1014)
                     /locus_tag="VGC82_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424853.1"
                     /translation="MKARNIGREERKADRDADTPRLREEPVTASVFLLGKTIRLLPNA
                     DGELRAADVDQPADPVGVERYLVKAFGDHLAEVRAAMEELAGRYDPAELNRIGFRLYE
                     RFRPDVPQGNEGWGAKAVLDVAKILAAV"
     gene            1920..2189
                     /locus_tag="VGC82_16160"
     CDS             1920..2189
                     /locus_tag="VGC82_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY0424854.1"
                     /translation="MSKAFIAEVIRQSTEVSGVTASRATNELLNAIVKELKKTGKFTL
                     PSFGTFTVHRTKARKALNPQTGASIRVRAGKTVRFKASPILKRAV"
     gene            3214..>3437
                     /locus_tag="VGC82_16165"
     CDS             3214..>3437
                     /locus_tag="VGC82_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424855.1"
                     /translation="MIDKAELIKLYNQHPRLTYKEISEKLGIKLGSLGNYLYHLQAMH
                     EIESRSETGKFRLMVKGEARLDRIEDLLKAG"
BASE COUNT          703 a         1052 c          995 g          687 t
ORIGIN      
        1 ccacggcggg gccaatggat catctcgtct gatgcccgac agggcaggtg gcggtggcaa
       61 gtggtgtgcc gcctttccat gtgctgcccg cctcggatgc gtctggacca ctggttcatg
      121 ttttgccgga tgacgcgcgg agttcgtctt cgcagcgcta tcgtgttcgc gtttcccgtg
      181 cgccagcgca tcccaaggca cgaccgtgga taccgccgca accatggcgg ccgtgcgaag
      241 ccaaactgac aggctccgca ttcgttgcac tcccctggcc acgcgtctca tcccccggcc
      301 tgtaacggga cgtatgcaac gcacggtgca cgatttcgtt cggctgaact cctccccaac
      361 ctaaaggagg gcttccgcat tcgccaccat cgcatgcctt cggccgccgc agcgcgggtg
      421 cgggcagtta gccacgcaac ttgcctctga acaaaccgat cgattgtctg gcgcgggcgc
      481 ccacggggcg acgcaatcga accactgcta tcggtgaacg gccggattgg gggtactccg
      541 cttcaaccga cgcggctggt gaggtatttg accttcgcgg cgtcgttcca gcatttggct
      601 tcatcgggga acgctccatg ctcaaacagc ggccaggatc ttcgcaacgt ccagtaccgc
      661 cttcgcgccc catccttcgt tcccctgcgg cacgtccggt cggaacctct catacagccg
      721 gaagccgatg cggttgagtt ccgccggatc atatcgtccg gccagttcct ccatcgcggc
      781 acgtacctcc gccagatggt cgccgaaggc tttgaccagg tagcgttcca cgccaaccgg
      841 atcagccggc tggtcgacat cagccgcccg caactcgcca tcggcattgg gcaacaaccg
      901 gatcgtcttg ccgagcagga acaccgacgc cgtcacaggc tcttcgcgca gccgtggcgt
      961 gtcggcatcc cggtcggcct tgcgctcctc ccgcccgatg ttgcgggcct tcacgcgcgc
     1021 cgctgagccg gccacagctc ggccaagtgt caaggccgtg tcagcgggat ggccaaggcg
     1081 ctcggccacc acagcgcccc acaaggtcag aacgggagcc cggttgacga tcactggcat
     1141 cagcgcagcg aagcgactaa tttcccgaac agcaagcggg gttccggtgc tgaccggcac
     1201 cattacgctc gcaaacgtcg catcacttgc gctccttcgc agcgacagcg gtttatagag
     1261 tgacgcagct atccgagttt tctgccgcag cagcgttgct gaggccaaat gagcgcgatt
     1321 gccagcagcg attttcagtc actccgggcg gcattgaccg ctgcggtatg aactctgtca
     1381 gccttgcttg tccggcctgg catgccgctt ccatgttgcc gaagcgctcg gatgagacat
     1441 aaagcggatc aacgcgttct tcgctgtaga cttcccagct aaacggcgtc gcgcttctct
     1501 cgacccgacg gatcaccacc ctgatggaaa gagcctcttt cgtaggtctc atgagacgat
     1561 tctgacctct caataggcgt ggtggtcaca agcagcgctg tcaacgacgt ggtttgatcc
     1621 tcacgcacac cgcaacatca tcctcatgtt gcatatgata cgaatttgca aagaacattg
     1681 ctgtgaccgt gctggtgccg ccggcggccg atccaccagg gcgccgcgcc ctctgtctca
     1741 tggcgtctgc caacacgggc ttggctgagc cgtcggtcat tgtcggcttc tgcaacgttt
     1801 catcacattt ccgctaaatc cgcgggtatt tatctaattg ttgttgcgtt ccgatacaga
     1861 tacgcatcaa acgatggaaa ttacgatatc gaaccagtaa cttccaccga ggtgcgtcca
     1921 tgtccaaagc ctttatcgcc gaggtcatcc ggcaatcaac cgaagtcagc ggcgtaaccg
     1981 catcccgtgc caccaacgag ttgctcaacg ccatcgtcaa ggaactgaag aagaccggga
     2041 agttcacgct ccccagcttt gggaccttta ccgttcaccg aaccaaagcc cgcaaggcat
     2101 tgaacccgca gacaggtgca tccatccggg tcagggccgg caaaaccgtc cggttcaagg
     2161 catcgccaat tctgaagcgg gcggtatagc gtaagaaacc agcttgcctg aatagtcgat
     2221 ctgatcccgc acaatcaccc acgctgccag tggatgcgtt cacaccgcat cgacgagatg
     2281 gagcgggcca tcaccgcgtc gatgatctgg tccgacatgc ccgtggcttc ttcgcgggca
     2341 ggggcggcaa gggctcctac ccgctgtcgc ggcggctttg caatgacgat cgcgtaagct
     2401 ggcaaagtcc tgagcctaat cgacctgcgg gtaccgcgtg acagaaggcg atcaataaaa
     2461 acgccgcata gggtgtgagc cgcttaacag cggtggggtc cggatccgga taacctgtgt
     2521 tcaacgatcg agcacacggg agagatccga tgttccgttt agctatttcc ctgattggtt
     2581 tcatcacaac cgcagccatg caccccgcca tcgccgatcc ggcttcagcc gcaccaccgg
     2641 tcaccgcaca gtatgcgccg gccaccgagc agcccgcaca ggtacaggtt ccggtctcgg
     2701 aaacaaacgc gccggttggc gctgggtggg gctagtcatt cccggaaatg cattgccgtt
     2761 gagcattgct cagcagaatt cccatgaaca tcaccggaag acgggggttt acgatagcgc
     2821 gtgcaataag cggattgatc cgctgacgcc gtgtcgtgga gccgatggtt gaggcagcgc
     2881 tacgtcgaac attaatatct gattatcaaa tcataacgat atgttgtatg acgcatagct
     2941 ggctgtgggt cgcccacggg tcattgtacc gccggacgct gcaggcgttc tgagactgcg
     3001 cccgcgttga gtcggcatga ctgaatctga cctgaatggc cagacattag gtttcggagc
     3061 ggaacgtgtg atgcgcggcg ggcgagcggc aattcgtttg gtcctgatga gtggcgcgcc
     3121 tgtgcaccga gttgccgcgc gagggtggag tgatgggcgt ccatttcgag atgtttgtgg
     3181 agaccgcggc cacgatgggg cagcggagcc atcatgatcg acaaggcgga actaataaag
     3241 ttgtacaatc aacacccacg gctgacctac aaggaaatat cggaaaaact tggcatcaag
     3301 ttaggctcgc tcggcaatta cctgtaccac ctgcaagcaa tgcatgagat cgagagccgt
     3361 agcgaaacgg gaaagtttcg tttgatggtc aaaggtgaag cgaggctcga tcggattgaa
     3421 gacctgctga aggccgg
//
LOCUS       DASWQN010000946         3084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2070630, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000946 DASWQN010000000
VERSION     DASWQN010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2070630"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VGC82_16170"
     CDS             complement(<1..568)
                     /locus_tag="VGC82_16170"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEY0424856.1"
                     /translation="MNSIVADRNLHIVPATRQPDSQSSLAREIELKFLVNESDFKTIQ
                     QSSLLGSGAGRGVTQRLHSVYFDTEDGHLRRHRIVLRMRRLRNNHVLTLKWDAKAGGG
                     TFERGEIEMPAPSPVPDPALLGAAIEAEILRVTEGRPLQPFFATDIKRVVRRITAGTS
                     ELEAAFDSGFIVFGAETMPVREIELELKA"
     gene            1134..1946
                     /locus_tag="VGC82_16175"
     CDS             1134..1946
                     /locus_tag="VGC82_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEY0424857.1"
                     /translation="MCTVVVLIRPTHVLLAANRDERLDRPWDQPDAYWPGHPGVIAGR
                     DRTAGGTWMGMNRHGVVATVLNRPGTLGPAAGKRSRGELPLLALDHRTAAAAAKAITD
                     LDAGLWRGFNMVLADSTGAFFIRGSGSGQPQAERLPSGVSMVTAHDPNDLNSPRTARH
                     LARFQEGEPPGPDQWGRWREILADRHGAWAEQINVEPRGGFGTVCSSFVSLPAAGRPI
                     WLFAPGPPHKVEFQPVFDGPGFGDAGLCDAGLCDAGFCNGDTAAIPAPLRDL"
     gene            1991..2755
                     /gene="purC"
                     /locus_tag="VGC82_16180"
     CDS             1991..2755
                     /gene="purC"
                     /locus_tag="VGC82_16180"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY0424858.1"
                     /translation="MARRRQLYEGKAKILFEGPEPGTLVQYFKDDATAGNGAKKGIIT
                     GKGVLNNRISEHLMVRLHEIGVPTHFVRRLNMREQLIREVEIIPLEVVVRNVAAGSLA
                     TRLGIVEGTRLPRSIIEYYYKNDALNDPMVSEEHITCFGWANTADLDDIVALTLRIND
                     FLSGLLLGVGITLVDFKLEFGRLWENDEMRIILADEISPDNCRLWDSKTNEKMDKDRF
                     RRDLGKVEEGYQEVARRLGILPEAGTRDLKGPELMQ"
     gene            2752..2994
                     /gene="purS"
                     /locus_tag="VGC82_16185"
     CDS             2752..2994
                     /gene="purS"
                     /locus_tag="VGC82_16185"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY0424859.1"
                     /translation="MKATVTVMLKAGVLDPQGKAIAHALGTLGFTGVEDVRAGKVLEI
                     ELAETDPAKARDAAEEMARKLLANTVIESFRVAIED"
BASE COUNT          586 a          982 c          986 g          530 t
ORIGIN      
        1 ccgccttcag ttccagttcg atctcacgca caggcatcgt ctcggcaccg aagacgatga
       61 aaccgctgtc gaacgcggcc tccagttcgc ttgtcccggc ggtgatccga cgcaccaccc
      121 gtttgatgtc ggtggcgaag aacggctgca gcgggcggcc ttccgtcacc cgcaggatct
      181 cggcctcgat cgctgccccg agcaacgcag gatcgggcac cggggaaggt gcgggcatct
      241 cgatctcgcc gcgctcaaag gtgccgccgc ccgccttggc atcccacttc agcgtcagca
      301 catggttgtt gcgcagacgg cgcatgcgca gcacgatgcg gtggcgccgg aggtgaccgt
      361 cttcggtgtc gaaatagacc gaatgcagcc gctgggtgac gccgcgcccg gcaccagacc
      421 ccagcaacga ggactgctgg atggttttga aatcggactc gtttaccagg aacttcaact
      481 cgatctcccg cgccaaggaa ctttggctgt cgggttgccg cgtcgccggc acgatgtgca
      541 gattcctgtc ggcgacgatc gagttcaatt ttgttacagc tcgcatgctt caggccaata
      601 gcagggaaag caaagggtaa ccacacccac gataatcgca ttcggaccat ggggttgctg
      661 cgtatgtcgc agcgcgataa tccatgtctg tcagaccacc ataccagcac caccaccata
      721 ccagcaccgt ggagatgcaa attgcatacc ggcgggtttg cccggcagcg gcggcttcaa
      781 ccgtcagccc caacgcccaa aatggctgat caggcagccg cgaatcatca agctcgaggt
      841 cgttacctgt tggcgacaca gagcttgccc cggagcttgc gttgatacgt cgcggtgccc
      901 cggcagggtc aagatgaagc gacttcctac cgggacagac agtaaccttg ttcagtcgca
      961 cctaatcgat gcagccgcgt gttaccaggc gtcccgccga actcgaacct cgtgaacagc
     1021 ccgagggtgg cggacgagat cggcgcgtat ccgaacggtg aaggtcggtc ccgatccgac
     1081 tccgcacgct ccgccgctgg cgccgtcggt tccgaagggc catcttgtca gccgtgtgca
     1141 ctgtcgtcgt cctgatccgc ccgacccacg tccttctcgc cgccaatcgc gatgagcggt
     1201 tggatcgacc ctgggaccag cccgatgcct actggcccgg ccaccccggg gtgatcgccg
     1261 ggcgggatcg cactgctggc gggacatgga tgggaatgaa ccggcatggc gtcgttgcga
     1321 cggtgctgaa ccgcccggga acgctggggc cggccgccgg caaacgcagc cgtggcgaac
     1381 tgcctttgtt ggcgttggat catcggacgg ctgccgcggc ggcaaaggcc atcaccgacc
     1441 tggatgccgg cctgtggcgc ggcttcaaca tggtgttggc cgactcaacc ggcgcgttct
     1501 tcatccgcgg ttcgggtagc ggccagcctc aggccgagcg cctgccgtcg ggcgtttcga
     1561 tggtcacagc gcacgatcca aacgacctga atagcccacg gacagcccgt cacctggcgc
     1621 gcttccagga gggggaacct ccggggccgg accagtgggg ccgctggcgc gagatcctgg
     1681 ctgaccggca cggcgcatgg gcggagcaga tcaacgtgga gccgcggggc ggtttcggca
     1741 cggtctgctc gtcttttgtc tcgcttcctg ccgccgggcg acccatatgg ctattcgcac
     1801 cgggcccacc gcacaaggta gaattccaac cggtctttga cggccccggc tttggagacg
     1861 ccggtctttg cgacgccggt ctttgcgatg ccggcttttg caatggggat accgctgcta
     1921 tacccgcccc gctgcgggac ctataaccgc gccgccgcct gagcgcggtg accaccgaag
     1981 gaaacccgcg atggcccgcc gccgccagct ctatgagggc aaagccaaaa tcctgttcga
     2041 aggacccgaa cccggcaccc tggtccagta cttcaaggat gacgctacag ccgggaatgg
     2101 cgccaaaaag ggcatcatca ccggcaaggg cgtcctcaac aaccgcataa gcgagcacct
     2161 gatggtgcga ctgcacgaga tcggcgtgcc gacgcatttc gttcgccgct tgaacatgcg
     2221 cgagcaactg atccgcgagg tcgagatcat cccgttggag gtcgtggtcc gcaacgttgc
     2281 cgccgggagc ctggcaacca ggctgggcat cgtcgagggc acccgcctgc cccgctccat
     2341 catagaatat tactacaaga acgatgccct gaacgacccg atggtgtccg aggagcacat
     2401 cacctgcttc ggctgggcca acaccgcgga cctcgacgac atcgtcgcgc tgacgctgcg
     2461 gatcaacgac ttcctgtccg gcctgctgct gggtgtcggc atcacactgg tcgatttcaa
     2521 gctggaattc ggccggctgt gggagaacga cgagatgcgg attatcctgg ccgacgagat
     2581 cagcccggat aactgccggc tttgggacag caagaccaac gagaaaatgg ataaggaccg
     2641 ctttcgccgt gacctgggca aggtcgagga aggctaccag gaggtcgccc gccgcttggg
     2701 catcctgccg gaagcgggca cacgcgacct caaagggccg gagttgatgc agtgaaggcg
     2761 acggtgacgg tgatgctgaa ggccggcgtg ctggaccctc agggcaaggc gatcgcccat
     2821 gccctgggga cgctcgggtt cactggcgtg gaggacgtgc gcgccggaaa ggtactggag
     2881 atcgaactgg ctgagacgga ccccgccaag gcccgcgacg ccgccgagga aatggcgcgc
     2941 aagctgctgg cgaacacggt catcgaaagt tttcgggtgg cgatcgagga ttaggaacag
     3001 gcttgctgct ttacgtcagg gaactcagtt cgagaaccgg catcgcgacg tgccgccgcg
     3061 ctgacgtgtg attgcgattt cgac
//
LOCUS       DASWQN010000947         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1278938, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000947 DASWQN010000000
VERSION     DASWQN010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278938"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VGC82_16190"
     CDS             <1..663
                     /locus_tag="VGC82_16190"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0424860.1"
                     /translation="IEKSTLLRRFENMVRDQRKQGRRCLLVIDEVQNLSLAAMEELRM
                     LSNIVVDGKASVQTILLGQPQFRPVLAGKDWEQLRQRVLASCHLGPMTSEETRGYIEH
                     RLRTVGWQGDPVWQDTAFAAVHRQTGGIPRRINTLCSRVLLFGALEETHAITAGIVDD
                     TAAELNRDLGAELEAATRPVLEDRDDLVRRIEQLERHAFRQEQAFKRVMDLLEANATG
                     QV"
     gene            663..1511
                     /locus_tag="VGC82_16195"
     CDS             663..1511
                     /locus_tag="VGC82_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HEY0424861.1"
                     /translation="MRNAMTVDVEDYFQVQAFAHCIRRQDWGRFPRRVDFNTNRVLDQ
                     FGAAGVKATFFTLGWVAERFPALIRRIVEDGHELASHGWDHTRVDSQHSEAFREDIRR
                     TRALLEDTGGVRVTGYRAATFSMGAQTMWAYPILRQEGYLYSSSINPIAHDLYGMPDA
                     PRVPFRPQADGVMEIPMTTVRLAGRNWPCSGGGYFRLLPTALYRSGLRWVNQREGQPG
                     IFYFHPWEVDPGQPRISNAGWKSRLRHYTNLSRMARDVDKLLRDFAWDRMDRVYAPLL
                     VETTAA"
     gene            1516..2553
                     /locus_tag="VGC82_16200"
     CDS             1516..2553
                     /locus_tag="VGC82_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HEY0424862.1"
                     /translation="MSIILRTLDAASEAAWDRFVQAMPGATFFHRAGWAKVIETAFGH
                     ATHFTFTERDGAITGVLPLGRVKTMLFGDTLISSPFCVYGGPVASDAESRAALIGHAE
                     RLLARTGAGALEFRDSIPAAEEDGWTDGPNLYVTFRKTIEADHERNMKAIPRKQRAMV
                     RKGIQNGLTSVANRDSDGLHRIYAESVRNLGTPVFSRRFFRILMAVFGDAVDVVTILD
                     QDTPIASVMNFYFRDQVLPYYGGGTALARSRAGNDFLYWETMRRAADRGCQLFDFGRS
                     KIGTGAFSFKHNWGFEAENLHYRFRLKPGRTIPDHNPLNPKYRMFIAAWKHLPIPVAN
                     LLGPHIVRGLG"
BASE COUNT          486 a          806 c          875 g          468 t
ORIGIN      
        1 attgagaaat cgacgttgct gcgccggttc gagaacatgg tccgcgacca gcgcaagcaa
       61 ggacggcggt gcctgctggt gatcgatgag gtgcagaacc tgtcgctggc ggccatggag
      121 gaactgcgca tgctgtccaa catcgtcgtt gacggaaaag cctcggtaca gacgatcctt
      181 ttgggtcagc cgcagttccg gccggtactt gccggcaagg attgggagca actccgccaa
      241 agggttctcg catcgtgcca tcttggacca atgaccagcg aggagactcg cggctatatc
      301 gaacatcggc tgcgcaccgt cggatggcag ggcgatccgg tctggcagga cacggccttc
      361 gcggcggtgc atcgccaaac cggcggaatc ccgcggcgga tcaacacgct ctgttcccgc
      421 gtgctgctgt tcggcgcact ggaggagacc catgcgatca ccgccggcat agtcgatgac
      481 acggccgcgg aactgaaccg cgacctcggt gcggaactgg aagcggcgac gcggcccgtg
      541 ctggaggatc gggacgattt ggtgcgccgg atcgaacagc ttgaacggca cgcgttccgc
      601 caggaacagg ccttcaagcg ggtgatggac cttctggaag ccaacgcgac aggacaggtc
      661 tgatgcggaa cgcgatgaca gtggatgtgg aggactattt tcaggtccag gcattcgccc
      721 attgcatccg ccgccaggac tggggccggt ttccgcgccg cgtcgatttc aacaccaacc
      781 gggttctcga ccagtttggc gcagccgggg tcaaggccac gttcttcacc ctgggctggg
      841 tcgcggagcg atttccggcg ctgatccggc ggattgtcga agacgggcat gaactggcta
      901 gccatggctg ggaccataca agggtcgatt cgcaacactc ggaggcattt cgcgaggaca
      961 tccgccgcac ccgggccttg ctggaggata ccggcggcgt ccgcgtaacc ggataccgag
     1021 cggcgacatt ctccatgggc gcgcagacca tgtgggcgta cccgatcctg cgtcaggaag
     1081 gctacctcta cagctcgagc atcaatccca tcgcccatga cctgtacggg atgccggatg
     1141 cgccgcgcgt gccgtttcgc ccacaggcgg acggcgtgat ggagatcccg atgaccacgg
     1201 tgcggctggc gggacgcaac tggccgtgct ccggcggcgg ctatttccgg ctgttgccga
     1261 ccgcgttgta ccggtcggga ctacgctggg tgaaccagcg ggaagggcag ccggggattt
     1321 tttatttcca cccgtgggag gtcgatcccg gccagccacg tatctcgaac gccggctgga
     1381 aatcgagact gcggcactac accaacctgt cgcgcatggc gcgggacgtg gacaagctgt
     1441 tgcgggattt cgcctgggac cggatggatc gcgtctatgc cccgctcctg gttgagacca
     1501 cggcggcctg agccggtgtc gattatcctc cgcacgctgg atgcggccag cgaggccgcc
     1561 tgggatcgtt tcgtgcaggc gatgccgggt gctacgttct ttcaccgcgc cggttgggcg
     1621 aaggtcatcg aaacggcgtt cggccacgcg acccatttca cattcaccga acgcgacggc
     1681 gccatcaccg gcgtcctgcc gctcggacgc gtaaagacga tgctgttcgg cgacacgctg
     1741 atctccagcc cgttctgcgt ctatggcggt ccggtggcgt ccgacgccga gagcagggcg
     1801 gcgctgatcg ggcatgccga gaggcttctg gcccgaaccg gggccggcgc actggaattt
     1861 cgcgattcga taccggcggc ggaggaggac ggctggaccg acggcccaaa cctttacgtg
     1921 acattccgca agacgatcga ggcagaccac gagcgcaaca tgaaggcgat tccccgcaag
     1981 cagcgggcga tggtacgcaa ggggattcag aacggcctga catcggtggc gaaccgtgac
     2041 tcggacgggc tgcaccggat ttacgccgag agcgtgcgca acctgggaac gccggtcttc
     2101 tcacgccgct ttttccgcat cttgatggcg gtgttcggcg atgccgtcga tgtcgtcacg
     2161 atcctggacc aggacacgcc gatcgcctcg gtgatgaatt tctatttccg tgaccaggtg
     2221 ctgccatact acggcggtgg cacagcattg gcgcgcagcc gggcgggcaa cgacttcctg
     2281 tactgggaga cgatgcggcg tgcggcggac cggggatgcc agttgttcga tttcggccgc
     2341 agcaagatcg gcaccggcgc gttttcgttc aagcacaact ggggattcga ggcggaaaac
     2401 ctgcattacc ggtttcggct gaaacccggg cggacaattc ccgaccacaa cccgctcaac
     2461 ccgaaatacc gcatgttcat cgcggcgtgg aagcacttgc cgatccccgt ggccaacctg
     2521 ttgggtccgc acatcgtccg agggctgggc tagggatgac gaagcccaac cttctgttcc
     2581 tgacccagcg cctgccgtac ccgaggatca agggcgagaa gatccggccg ttgca
//
LOCUS       DASWQN010000948         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1410985, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000948 DASWQN010000000
VERSION     DASWQN010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410985"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VGC82_16205"
     CDS             <1..659
                     /locus_tag="VGC82_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEY0424863.1"
                     /translation="NDYVGKGLSGGRVVVRQPANVKRNPMENIIVGNTVLYGAVAGEA
                     YFQGVAGERFAVRNSGAVAVVEGCGDHGCEYMTGGVVVVLGDTGRNFAAGMSGGVAYV
                     YDDKARFGKLCNPAGVELEKIGVAELGLADEPGRPRQRSASVDDSGMGDPLRFDAERL
                     RIVVERHLLFTGSARARALLEDWEGALSRFVKVMPRDYKRALLDLKAEAAASATVAAE
                     "
     gene            737..>1885
                     /locus_tag="VGC82_16210"
     CDS             737..>1885
                     /locus_tag="VGC82_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY0424864.1"
                     /translation="MGKITGFLEIDRHDRAYEKTELRVKSFKEFVKPLPPEELNGQVA
                     RCMDCGIPFCHNACPVNNQIPDWNNLVYREQWYQASVNLHSTNNFPEFTGRICPAPCE
                     AACTLNIDDNPVTIKTIECAIVDRAWEEGWIVPSPPAVKTSKAVAVVGSGPAGMACAQ
                     QLARAGHAVTLIERQDRIGGLLRYGIPDFKMEKHLIDRRVDQMAAEGVEFRTGIEVGK
                     DVTVDQLLADYHAVVLTGGAEWPRNLDVTGRELDGIHYAMDFLTQQNKRVAGDDETIA
                     APEGAITAKGKHVVVIGGGDTGSDCIGTSARQGAASITQIEIMPKPPVKEDKAMVWPE
                     WPTKLRSSHAHEEGVERDWAVLTKHAIGSDGKVTALDCVRIEWERTPDG"
BASE COUNT          364 a          593 c          608 g          320 t
ORIGIN      
        1 ccaacgacta tgtcggcaaa ggcctgtccg gtggccgcgt cgtcgtccgc cagccagcga
       61 acgtcaagcg caatccgatg gagaacatca tcgtcggcaa caccgtgctg tatggcgcgg
      121 ttgccggcga agcctacttc caaggcgtcg ccggtgagcg cttcgccgtc cgcaattcgg
      181 gcgccgtcgc ggtcgtcgag ggctgtggcg accatggctg cgaatacatg actggcggcg
      241 tcgtcgtcgt gctgggtgac accggccgca atttcgccgc cgggatgtca ggcggggtcg
      301 cctatgtgta cgacgacaag gcgcggttcg gaaaactctg caacccagca ggcgtggagc
      361 tggaaaaaat cggcgtggcc gaacttggtc tggccgacga acccggacgg ccacggcagc
      421 gctcggccag cgtggatgac agcggcatgg gtgatccgtt gcggttcgac gccgaacgcc
      481 tgcgcattgt cgtcgagcgg caccttctgt tcaccggcag cgcccgagcg agggcattgc
      541 tggaggattg ggaaggggcg ttgtcccggt tcgtcaaggt catgccgcgc gactacaaac
      601 gcgcgctgct cgacctgaag gccgaagccg cggcatccgc cactgtcgcc gccgaatagt
      661 aacagttccg ccaacgcatg atgacccgtc cctgcttggg gcggtggcgt ctgttggtga
      721 ccatctggag acgatcatgg gtaagattac cggtttcctc gaaatcgacc gacacgaccg
      781 cgcctacgag aaaaccgaac tgcgcgttaa gtctttcaag gagttcgtca agccgctgcc
      841 accggaggag ctgaacggcc aggtcgcccg ctgtatggac tgcggtattc cgttttgtca
      901 taacgcatgt ccggtcaaca accagatccc cgactggaac aacctggttt accgggagca
      961 atggtatcag gcgtcggtca acctgcactc caccaacaat ttccccgagt tcacaggccg
     1021 catttgcccc gccccgtgcg aggctgcctg caccctgaac atcgacgaca acccggtcac
     1081 catcaagacg atcgagtgcg ccatcgttga tcgcgcctgg gaagaaggct ggatcgtgcc
     1141 ttccccgccg gcggtgaaaa ccagcaaggc cgttgccgtc gtcggctccg gacccgccgg
     1201 catggcgtgc gcccagcaac tcgctcgcgc cggtcatgcc gtgacgctga tcgaacggca
     1261 ggatcgcatc ggcggcctgc ttcgctacgg cattcccgac ttcaagatgg agaaacacct
     1321 gatcgaccgc cgcgtcgacc agatggcggc cgaaggggtc gaattccgca ccggtatcga
     1381 agtcggcaag gatgtcacgg tcgatcagct actggccgat taccatgccg ttgtgctgac
     1441 cggtggcgcc gagtggccgc gcaacctcga tgtcactggc cgcgaactgg acggaatcca
     1501 ctacgcgatg gacttcctga cgcagcagaa caagcgggtg gccggcgacg acgaaaccat
     1561 cgctgccccc gaaggcgcga tcaccgccaa gggcaagcat gtcgttgtca tcggtggtgg
     1621 cgataccggc tccgactgca tcggcacctc cgcccggcag ggtgcggcct cgatcaccca
     1681 gatcgagatc atgcccaagc ccccggtcaa ggaagacaag gcgatggtat ggccggagtg
     1741 gccgaccaag ctgcggtcgt ctcacgccca tgaggagggc gtcgagcggg attgggcggt
     1801 cttgacgaag catgccatcg gcagcgacgg caaagtcact gcgctcgatt gtgtccggat
     1861 cgagtgggaa cgcacgccgg atggg
//
LOCUS       DASWQN010000949         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_486423, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000949 DASWQN010000000
VERSION     DASWQN010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486423"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="nadA"
                     /locus_tag="VGC82_16215"
     CDS             complement(<1..681)
                     /gene="nadA"
                     /locus_tag="VGC82_16215"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY0424865.1"
                     /translation="MSATVLLERVAPLYDRVKTLIPPIEWPVFAEDIEAILRLKRERD
                     AVILAHNYMTPEIFHCVADIVGDSLALAREAMKVDAKVIVLAGVHFMAETAKLLNPAK
                     TVLIPDSRAGCSLAESITAADVRLMRERHPGVPVVTYVNSSAAVKAESDICCTSGNAL
                     KIVESLGVDTVIMLPDEYLAQNIAAQTKVKVLTWAGHCEVHERFTAAEVRALRTDYPG
                     VTILAHPEC"
     gene            complement(826..1644)
                     /locus_tag="VGC82_16220"
     CDS             complement(826..1644)
                     /locus_tag="VGC82_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HEY0424866.1"
                     /translation="MIAFLGSVFSPYYAWARRRSGGDPMRHCALNVALYGKARRWAMT
                     ERRAGAVERGPGFLAIGPSSLSWDGTGLTVRIEEIAVPWPRRIRGTLRLYPSAVESRV
                     VELDSAGRHRWQPIVPCARVEVALEQPNVSWSGAAYFDTNMGERPLEADFVRWDWSRS
                     RVSGGTAVLYEVERRDARLTLAMRYDDAGGVADFAPDQAVALPKTLWRLPRRISAGSP
                     AVLETLEDTPFYARSIIAADLLGGRVSAMHESLAMDRFIAPWVQAMLPFRMPRI"
     gene            complement(1716..>3085)
                     /locus_tag="VGC82_16225"
     CDS             complement(1716..>3085)
                     /locus_tag="VGC82_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0424867.1"
                     /translation="AYVGGKIRVDPAGIDSGPTVLTMRWVFEALFADAGSSLADHLTL
                     HPLDLLARHAWSDTERLDLFADLEQSIAAIGDFAGRAEADGYRSFCSRAAVVYRTLEA
                     PFMRAQKPTAFSLTAGTGISAMRAASPFSTLWQALGEHFRDPRLRQLFGRYATYSGSS
                     PFQAPATLMLIAHVEQQGVWLVEGGMVRLGQALTALAERLGAVVRLDTPVAEILVSRG
                     QVTGVLLEDGERLDADAVVCNADYAALADGAFGQAATRSVPVAKRAGRSLSAVTWTLS
                     AATEGFPLARHNVFFGGDYAAEFSSVFRHGRLPHDPTVYVCAQDRPGETPAGGAERLL
                     VLINAPANGDHGQEISPSEIEQCATRTFGLMERCGLRVHRSPDAVITTPGTFAKLFPA
                     TGGALYGQAVHGSMAAFRRPGSRSALPGLYLAGGSVHPGPGVPMAVLSGRLAAASLLQ
                     DRTSR"
BASE COUNT          611 a         1105 c          856 g          513 t
ORIGIN      
        1 acattccggg tgcgctaaga tcgtcacccc cggataatcg gtgcgcaaag cccgcacctc
       61 ggcggcggtg aagcgttcat gcacctcgca atgcccagcc catgtcagca cctttacctt
      121 ggtctgcgcc gcgatatttt gcgccaggta ctcatccggc agcatgataa cagtatctac
      181 accgagcgac tcgacgatct tcaacgcatt gccggacgtg cagcaaatat ccgactctgc
      241 ctttaccgcg gcggacgaat ttacgtatgt cacgacaggc acgccggggt gacgttcccg
      301 catcaaccgc acatccgccg ccgttatgga ctccgccagc gagcatcccg ctcgactgtc
      361 gggtatcagc accgttttgg ccgggttcag cagcttcgcc gtctccgcca tgaagtgcac
      421 gccggccagc acgataacct tcgcatccac cttcatcgcc tcccgagcca aggccaggct
      481 gtcgccgaca atgtctgcga cacagtggaa aatctccggg gtcatgtagt tatgcgccag
      541 gatcaccgca tcgcgctccc gcttcaagcg caggatggcc tcgatgtcct cggcgaacac
      601 cggccattcg atgggcggaa tgagcgtctt gacccggtcg tagagtggag cgacgcgttc
      661 gagcagcacg gtagcggaca tttgagtccc tcctgtctaa tgctcttttt gagcattact
      721 ggagcttatg ctagcgatga gtataaggat gtcaagcgct acctatcttc gtccgctgcc
      781 acgcctcccc gtcgattggc ggcccaatga cgtccgcccg accagctaaa tccgaggcat
      841 tcggaacggc agcatcgctt gcacccaggg ggcgatgaac cggtccatcg ccaggctttc
      901 atgcattgcc gagacgcggc cgcccagcag atcggcggcg ataatcgagc gggcgtagaa
      961 cggcgtgtct tccagcgtct caagcaccgc aggcgaaccg gcgctgattc gccgaggcag
     1021 ccgccacaac gtcttcggca acgcgaccgc ctgatccgga gcgaaatccg ccacgcctcc
     1081 ggcatcgtca taccgcatcg ccaaggtcag cctcgcgtcc cgccgttcga cctcgtacaa
     1141 aaccgcggtt ccgccggaca cccgcgatcg gctccaatcc cagcgcacaa aatcagcctc
     1201 caaaggccgc tcgcccatgt tggtatcgaa gtatgccgcc ccggaccagg aaacgttcgg
     1261 ctgttctaaa gccacctcca cccgcgcaca ggggacgatc ggctgccatc gatgccggcc
     1321 cgccgagtcc agttccacta cgcggctttc caccgccgag ggataaagcc gcagcgtccc
     1381 ccgaatccgc cgcggccacg gcaccgcgat ttcctcgatc cgtaccgtca ggccagtgcc
     1441 gtcccatgat agcgaactcg gtccgatcgc caggaatccc ggcccccgct ccaccgcgcc
     1501 cgcccttcgc tccgtcatgg cccagcgccg agccttgccg tagagtgcga cgttcaacgc
     1561 gcaatgccgc atcgggtcgc caccgctacg ccgccgtgcc caggcgtaat agggcgaaaa
     1621 cacactccct aaaaaggcga tcaaggtgat tccataagcg ccatcattgc tgagcgcatc
     1681 gacataccac caggcatacc cccctgccgg cggcgttatc tggaagtccg gtcctgcaaa
     1741 agactcgccg cggccaaccg cccggataac accgccatcg gcacccccgg ccccggatgc
     1801 acgctccccc ccgccagata caatcccggc agggcactcc gtgatcccgg ccgacggaac
     1861 gccgccatcg atccatgcac cgcctgcccg taaagcgctc cccccgtcgc cggaaataat
     1921 ttggcgaacg tccccggagt ggtgatcacc gcgtccggac tccggtgcac ccgcagtccg
     1981 catcgttcca tcaggccgaa ggtcctggtc gcgcattgtt caatctccga cggtgaaatt
     2041 tcctggccat gatcgccatt ggccggggcg ttgatcaaaa ccaggagcct ttcggccccg
     2101 cccgccggtg tttcgcccgg gcgatcctga gcacacacat agacagtggg atcgtggggc
     2161 aatcgcccgt gccggaacac ggaactgaac tcggcggcgt agtcgccgcc aaagaacacg
     2221 ttatggcgag ccaacggaaa cccctcggtc gcggctgaca atgtccaggt caccgctgac
     2281 agcgaccgcc ccgcacgctt cgccaccggc actgatcgtg tggctgcttg gccgaaggca
     2341 ccgtcggcca acgctgcgta gtcggcattg caaacgaccg cgtccgcatc gaggcgctca
     2401 ccgtcttcca gtaagacccc tgtcacctgg ccccggctga ccaggatctc ggcaaccggt
     2461 gtatccaagc gcaccaccgc gcccaggcgt tcggcaagcg ccgtcagtgc ctggcccaac
     2521 cgcaccatcc cgccttccac cagccagacg ccctgctgct ccacatgcgc gatcagcatc
     2581 agcgtcgccg gcgcctggaa tggtgatgac ccgctgtatg tcgcatagcg tccaaacagc
     2641 tgccgcaacc tcggatcgcg aaaatgctcc ccaagggcct gccacaaggt ggaaaaaggt
     2701 gacgcggctc gcatcgccga tatcccggtg ccagcggtca gcgagaatgc cgttggtttc
     2761 tgggcgcgca tgaacggcgc ctccagcgtc cggtacacaa cagctgcccg cgaacagaaa
     2821 gaccggtatc catcggcctc ggcgcgtccc gcgaagtcac cgatcgccgc aatggattgc
     2881 tccagatcag cgaagagatc gagccgttcg gtgtcactcc aggcatggcg cgccagcagg
     2941 tccaacggat gcaatgtcaa gtgatcggcc aaggacgaac cggcatcggc gaacagcgcc
     3001 tcgaacaccc agcgcatcgt caggaccgtt ggaccggaat cgatcccggc cggatccacc
     3061 cggattttgc ccccgacgta agccg
//
LOCUS       DASWQN010000950         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_618799, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000950 DASWQN010000000
VERSION     DASWQN010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618799"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..871
                     /locus_tag="VGC82_16230"
                     /pseudo
     CDS             116..871
                     /locus_tag="VGC82_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(878..2134)
                     /locus_tag="VGC82_16235"
     CDS             complement(878..2134)
                     /locus_tag="VGC82_16235"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY0424868.1"
                     /translation="MRRIGLLAAAVLSLLASGASAGSSTREAQTQRALRDAERAGSEQ
                     LAAHRDAAERAAHAAAVSIQLAQKRVEAAAKLRHAEAATADIAAKIDVLAQRRLDAEQ
                     RVQARAKAMQPLLPLIERLSLYPAETLLAVPAAPDVALRGVLVLQSLSRQIEVEAEAL
                     RRDQAEVDAASAALRGEEPRLNDAVTLQKREADLLDQQIAAAHKDAAAAEAEANGAAS
                     QAAAAASRMETLRSALAAIEAQRHADEANAREEAARAERQKRQAEVQRAHTREAMAAR
                     PTGAGTIASGAGPRAQLQPPVIGVVVKGWGEQTDGGPATGLSYHAPPGAHVISPCGGR
                     VVFAQGFRSYGLLLIVDCGGGYHVVLSGFERLDVKLGQSLLPGEPVGVMPGWEPGGTG
                     HRPALYVELRHDGTPVNPAPWLRSNG"
     gene            complement(2165..>3561)
                     /locus_tag="VGC82_16240"
     CDS             complement(2165..>3561)
                     /locus_tag="VGC82_16240"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0424869.1"
                     /translation="RCSNCGHRVPVVDGIVDFLDGRLPLPSGTDRYRGAVRADGSSGP
                     DLLNRVKGAAGGRWPAAMGDVIEFGCGPMTHSIAADEIIRSMIVLDSELSLLQACRAG
                     VLAGSPDRPLSFATLGGGHSVIRDSVADTVVGSALLSGISDVRAFLAMVHRALKPGGR
                     AMFVVPNRRYYQAICQALAEALVQLFARHGAWPEGCGSTMNFLADSRRLLIHRDDPGF
                     LISLTEKYLFDSDALEDLGKETGFAQAEIIPLDPDPAGAETMAGICQHAGAPDDFARE
                     IGALAASTGRPYLSLLSCQDSSALSLLWLTKASGPEVRVYTSRRTAPPAGHPGLDAAL
                     GGVQPRWSIELLARDTPDGILVTVGGWCLSNVDVRWVRITLDGVARHTPVWRPRADVH
                     EVLNRGRIYHPMNALCSGIEGDLLFTDASPRTMGAKDTGWSLGVHIVLASGLVMTGPA
                     PERLALDEPVVVVS"
BASE COUNT          663 a         1202 c         1070 g          626 t
ORIGIN      
        1 gtatccagga caacgtggag cttgagccgt acaactacgt ctttgccttg aaggaaatcg
       61 ccgccgcgaa gggcgagctt cgtcccttga tgatgctggc gatcagcccg gagatcaggc
      121 gccgcaactt cccggcgaga aaaccgtaga gccgacgttc ggcctgcccg cgtgctggat
      181 cgccccggcg ctggcggaac aggcgcgttc ggcaaactgg accgtcgtcg atccggccac
      241 tgtgctaacc acccatctgg ccgaaatcgt gaaggagaac atcgccgaat tcctgtccta
      301 tacggagact cagaaaattc tggcaggcct gccgcacgat cagcaaaagc tggttgccga
      361 tctcgttcca tcggtcatca cgctcggcgg attgcaacgt gtgctgcaaa tgctcctggc
      421 cgagcggatt tcgatccgcg atatgccgac gatcctggaa gcggttcagg aggcttgcgc
      481 caacaacgcg aaatccgtcg gccctattgt cgcgcatgtg cggacgcggc tttcgcggca
      541 gatcagcgac agcctgctcg ggccaggcgg atatctggca gtcatcatca tctctcagga
      601 atgggaggat attcttgctg gggcgctcgt cggtccagcg gacgatcgcc agttggcgct
      661 gggtcaggac aaattacggg agctcctgga gcggatacgc tccgtcgtcg attccacggt
      721 gcaaaacggg gagaaggcgg cactgctgac gagcatgcaa atccgctttc atgttcattc
      781 cgtcgttgac cggatcaggc tcaacgttcc ggtcattgcg cagccggagg tccatcgccg
      841 cgcccaggtc aaagtcgtgg gagttatcta aaacaggtca accattggat cgcagccaag
      901 gcgccgggtt caccggcgtc ccatcatggc gcaattccac atacaacgcc ggtcgatgcc
      961 cggtcccgcc aggttcccag cctggcatta ccccgaccgg ttcgcccggc aacaggcttt
     1021 gacccagttt cacatcaagt cgttcgaatc cagaaagcac gacgtggtag ccaccgccgc
     1081 aatcgacgat cagcagcaac ccatagctgc ggaacccttg cgcgaacacc acccgcccgc
     1141 cacagggtga gatcacatgg gcgcccggtg gtgcgtgata cgacaggccg gtcgcggggc
     1201 ctccatcggt ctgctctccc cagcccttca cgaccacacc aataaccggg ggttgcagtt
     1261 gcgcacgcgg tccagcaccc gacgcgatgg tgcctgcacc ggtcggacgt gccgccatgg
     1321 cttcccgtgt atgcgcccgt tgcacttctg cctgacgctt ctgccgttcg gctcgagcgg
     1381 cttcttcccg ggcatttgct tcatcggcgt gccgctgtgc ttcaattgcc gccaatgcgg
     1441 aacgcagtgt ctccatacgg ctcgcggcag cggccgcttg acttgcagcc ccgttcgcct
     1501 ccgcctccgc tgccgcggcg tccttatgtg ccgccgcaat ctgctgatcc aacagatcgg
     1561 cctcgcgctt ctgcagcgtc acggcatcgt tcaggcgtgg ttcttcgcct cgcagcgcgg
     1621 cagaagccgc atccacctca gcctggtcgc ggcgtagcgc ttcggcctcg acttcgattt
     1681 gccgtgacag gctttggagc accaatacgc cccgcagagc cacatccggg gccgccggca
     1741 ccgccaacag tgtttcagcc ggatagagcg acagccgttc gatcagcggc agcagaggct
     1801 gcattgcttt cgcccgcgcc tgcacacgct gttccgcatc cagccgccgc tgcgccagta
     1861 catcgatctt cgcggcgata tccgctgtcg ccgcctccgc gtggcgcaac tttgccgccg
     1921 cctcgacgcg tttttgtgcc agctgaatcg agactgcagc ggcatgtgcg gcccgctcag
     1981 ccgcgtctcg gtgcgccgcg agctgttcgc ttccagcacg ttcggcatcg cgcagtgcac
     2041 gttgggtctg ggcctcgcgg gtggatgagc cggccgacgc cccggacgca agcaggctca
     2101 gcacggctgc tgccagaaga ccgatacgac gcatcaccgg catctccgcc gattcatatg
     2161 ggcgctaaga cacgaccacc accggctcgt ccagggcaag tcgttcagga gccgggcctg
     2221 tcatgaccaa gccactcgcg agcacgatat gcacgccgag cgaccatcct gtgtctttcg
     2281 cgcccatggt cctcgggctg gcgtccgtga acagcaaatc gccctcaatc ccgctgcaaa
     2341 gcgcgttcat cgggtgatag atgcgcccgc ggttaagaac ctcgtgcaca tcggcgcggg
     2401 gacgccagac cggcgtgtgt cgcgcaacac cgtcgagggt gatgcggacc caccgcacat
     2461 ccacattgct gaggcaccag ccacccaccg tcacaaggat accgtccgga gtatccctgg
     2521 ccaatagttc gatggaccag cgcggctgga cgccgcccag ggccgcgtca agacccggat
     2581 gccccgctgg gggcgccgtt cggcggcttg tataaacgcg cacctccggc ccggatgcct
     2641 tcgtcagcca cagcaggctg agtgccgacg agtcctgaca gctaagaagg ctcaggtaag
     2701 ggcggccggt ggaggcggca agtgcgccga tttcccgggc gaaatcgtct ggtgccccgg
     2761 catgttgaca gatcccggcc atcgtctccg cgccggcggg atcgggatcc agcgggatga
     2821 tctcagcctg tgcgaagccg gtctccttgc ccagatcctc aagcgcgtca ctgtcgaaaa
     2881 ggtacttttc cgtgagcgaa atcaagaacc cagggtcgtc gcggtgaatc agcaatcggc
     2941 gtgaatccgc caggaagttc atcgtggatc cgcagccttc cggccacgct ccatgccgag
     3001 cgaagagctg gacgagtgcc tccgccaggg cctggcaaat tgcttggtaa tatcgccgat
     3061 tcggtaccac gaacatcgct cggccgcccg gcttcaacgc gcgatgcacc atcgccagaa
     3121 aggcgcgcac gtcactgatc ccggacagca acgcgctgcc gaccacggta tccgccacgg
     3181 aatcgcggat gacgctgtgg ccgccgccca gcgtcgcgaa agatagggga cgatccgggc
     3241 tgccagccag gacaccggcg cggcaggcct gcaacaggct aagctcgcta tcgagcacaa
     3301 tcatgctgcg gataatttca tccgcggcga tggagtgggt cattggcccg cacccgaact
     3361 cgatcacgtc gcccattgca gcgggccaac ggccgcccgc cgcaccttta acccggttca
     3421 gaaggtcagg ccctgacgat ccgtccgccc gcactgcgcc gcgatatcga tcggtgcccg
     3481 atggcagcgg cagccggcca tcgagaaaat caacgatccc atcgacgacc ggaaccctat
     3541 ggccgcagtt cgagcaacga a
//
LOCUS       DASWQN010000951         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1806770, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000951 DASWQN010000000
VERSION     DASWQN010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806770"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..584)
                     /locus_tag="VGC82_16245"
     CDS             complement(33..584)
                     /locus_tag="VGC82_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY0424870.1"
                     /translation="MSNKTWPYGTVFSLDGVTPQIADDAFIAPTAAIIGDVVIGSETG
                     VWFHCLVRGDMNSICIGARTNIQDGSVIHIDSGEFSTFIGDDVTVGHNAVIHACTLKN
                     RAFVGISATVLDGAVIEEGGMLAAGGLLTPGKVIGPNELWTGSPAKLRRVMEPEERAR
                     FDRNALVYRELAKKFRAGLKVVG"
     gene            complement(622..1569)
                     /gene="argC"
                     /locus_tag="VGC82_16250"
     CDS             complement(622..1569)
                     /gene="argC"
                     /locus_tag="VGC82_16250"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY0424871.1"
                     /translation="MTTVFIDGESGTTGLGIRERLGAMPQVTLKSLPFERRRDPSARR
                     DIMAEVDLIVLCLPDDAARESAAMAASLGNAAPKVLDASTAHRIAPDWVYGFAEMFPG
                     QAERIAEARNVANPGCYPTGAIGLIRPLIDAGLVPADYPITINAVSGYSGGGKAMIQA
                     HETTLGPAFELYGLGLEHKHVPETQLYGGLTRRPIFVPSVGHYLKGMLVSIPLHLDTL
                     PGKPVGADLRAALDARYAGCDLVRVMPPAPDGKLEPEALNDTDRLELTVHANEKYRQA
                     VLVARLDNLGKGASGAAVQNIALMLGLHTGVGARELLDA"
BASE COUNT          305 a          546 c          640 g          341 t
ORIGIN      
        1 gctgccggca aaccggagcg gcggtggcga tgttatccaa ccaccttcag gccggcgcgg
       61 aatttcttcg ccagttcgcg atacaccaac gcgttgcggt cgaagcgggc gcgttcctcg
      121 ggctccatga cacgtcgcag cttggcgggg gagccggtcc acagctcgtt tgggccgatc
      181 accttgcccg gggtcagcag gccgccggcg gcgagcatgc cgccttcctc gatgacggcg
      241 ccatccagca ccgtcgcact gatacccacg aaggctcggt ttttcaacgt gcaggcatgg
      301 atcacggcgt tgtggccgac ggtcacgtcg tcgccgatga atgtcgagaa ttcaccggag
      361 tcgatgtgga tgaccgaccc gtcctgaatg ttggttcgcg cgccgatgca aatggagttc
      421 atgtcgccgc ggaccaggca atggaaccag acgccggttt ccgacccgat cacgacgtcg
      481 ccgataatgg cagccgtagg ggcgatgaag gcgtcgtcgg cgatttgcgg ggtgacgccg
      541 tccagggaga aaacggtgcc atacggccag gttttgttag acatcgtttc gtccggtatc
      601 gttttgttag gcatcgtttt gtcaggcatc cagcaactcc cgagcaccta cgcctgtatg
      661 caaaccgagc atgagggcga tgttctggac ggcggcaccg gatgcccctt ttccgaggtt
      721 gtccaggcgc gcgaccagca ccgcctggcg gtatttttcg ttggcgtgga ccgtaagttc
      781 cagccggtcg gtgtcgttca gcgcctcggg ttccagtttg ccgtcgggtg cggggggcat
      841 gacgcggacc aggtcgcagc cggcgtagcg ggcatccagc gcggcccgaa ggtcggcgcc
      901 gacgggcttg cccggcagcg tgtccaggtg cagcgggatg gaaaccagca tgcccttgag
      961 gtagtgcccg accgagggca cgaagatcgg gcggcgggtc aggccgccat aaagctgtgt
     1021 ttcgggcaca tgcttgtgct ccaggccaag gccatagagt tcgaacgccg gccccagggt
     1081 ggtttcgtgc gcctggatca tcgccttgcc gccgccggag tagccggaga cggcgttgat
     1141 ggtgatggga taatcggcgg gaaccaggcc ggcgtcgatc agtgggcgga tcagcccgat
     1201 tgctccggtt ggatagcagc cgggattggc cacgttcctg gcctcggcga tgcgttcggc
     1261 ctggccggga aacatctcgg cgaagccata gacccagtcg ggcgcgatcc ggtgggcggt
     1321 gctggcatcc agcactttcg gcgcggcatt gcccaggctg gcagccatcg cggcggattc
     1381 gcgggcggcg tcgtcgggca ggcaaaggac gatcaggtcc acctcggcca tgatgtcgcg
     1441 cctggcggag gggtcgcggc gtcgctcaaa cggcaggctt ttcagggtca cctggggcat
     1501 agcgcccagg cgctcgcgga tgcccaggcc ggtggtgccg gactcgccgt cgatgaagac
     1561 cgtcgtcatg cgttgcaaac tccttcctcg gcggggaaat tcgcggggcg gcggcggaaa
     1621 agcaagaggg gtccgcgcgg ggctggcccg acagatgggc gatacggatg ggcaacacgg
     1681 acaggcggac aagcttgaca tcgcggaagc ggacaactac caagcgcgcc ttgttaggtt
     1741 ccgtcttcgt cagtcaggcc tatcttcgtc caggcctatc ttcgtcagtc aggctcgtct
     1801 ttgtcagtca ggctcgtctt tgtcagtcag gc
//
LOCUS       DASWQN010000952         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_355605, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000952 DASWQN010000000
VERSION     DASWQN010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355605"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..>1798
                     /locus_tag="VGC82_16255"
     CDS             133..>1798
                     /locus_tag="VGC82_16255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424872.1"
                     /translation="MSFPLLTTRRFGPLCLSQSCGAFNDNLVKNAMIVLAIFKLSAGG
                     AGLAALAGALFIAPYALLSATAGQLADRFDKSRLIQATKAAEVLLMAAAAYAFLANNV
                     PALLAVLFGLGVQATMFGPLKYGILPDHLRADELLAGNGLIEASTFLAILAGTVAGGA
                     LILLDAGPAIVAATGAGVSLIGLAAAFGVPPAPAADPALLIGRNILAETVSVTRQAAA
                     NRAVWLAILGLSWFWAIGATLLSEFPSIARDVLGAEGQVVTLLLTMFAVGIGVGSMLC
                     ARLLHGEVSARHVPFAAFGITLFTWDFGSTAIGAGNLSLTTIGAILHSPTGWHMLADL
                     LLLATCGGLYSVPLYAILQEQSKPSHRARTIAANNIVNAIMMVAGSAFVAACSAAGLS
                     APTVLRIAAVANFAVALWIVRLLPQTFFRTLFRWYFRLFHQTSIEGLENVAAPGERSI
                     LVINHLSFLDGCLVAAFLPGDLVFAIDTAQARRFWFLKYVIDVFPIDPNSPMAIRAMV
                     KAVREGRRLAVFPEGRITLTGALMKIYDGPGMVADRAGASVVAVRID"
BASE COUNT          252 a          627 c          558 g          361 t
ORIGIN      
        1 atcggcacct ggcaccgcgt gcataccggc aagacatttg ccgaggccct ccacatcctg
       61 gaatcggacc cggtcctgtt cccctattga acgcacgcgc agtggccaaa ccgccttcgg
      121 aaagtcctga ccatgtcgtt cccgctgctg acgacccgcc gcttcggccc gctgtgcctg
      181 tcgcagagct gtggcgcctt caacgacaat ctggtgaaga acgccatgat cgtgctggcc
      241 attttcaagc taagcgcggg tggcgccggc ctcgcggctt tggccggtgc cctgttcatc
      301 gccccctatg cgctgctgtc ggcgacggcg ggccaactgg cggatcgttt cgacaagtcc
      361 cgcctgatcc aggcgacgaa ggccgccgag gtcctgctga tggcggcggc ggcctatgcc
      421 ttcctggcca acaatgtgcc cgccctgctg gcggtgttgt tcggtctcgg cgtgcaggcg
      481 acgatgttcg ggccgctaaa atacggcatc cttccggacc atctgcgcgc ggacgaactg
      541 ctcgccggca acggcctgat cgaggcgtcc accttccttg cgatcctcgc cggaaccgtc
      601 gcgggcggag cgctgatcct gcttgatgcc ggtcccgcga tcgttgccgc gaccggcgcc
      661 ggcgtgtcgc tgatcggcct cgccgccgcg ttcggcgttc cgccagcccc ggccgccgat
      721 cccgccttgc tgatcggccg gaatatcctg gcggaaacag tgtcggtcac ccgccaggcc
      781 gcggccaacc gcgcggtgtg gctagccatc ctcggcctga gctggttctg ggccatcggc
      841 gccaccctgc tgtcggaatt tccctccatc gcccgagacg tgctgggcgc cgagggacag
      901 gtggtgacct tgctgctgac catgttcgcc gtcggcatcg gcgtcggttc catgctttgt
      961 gcccggctgc tgcatggcga ggtcagcgct cggcatgtcc cattcgccgc attcggcatc
     1021 accctgttca cctgggactt cggctcgacg gcgatcggcg ccggcaacct gagcctgacg
     1081 acaatcggcg ccattctgca ttcccccacc ggctggcaca tgctcgccga cctgttgctg
     1141 ctggcgacct gtggcgggct ttattcagtc ccgctctatg ccatcctgca ggagcaatcg
     1201 aagccgtcgc accgcgcccg cacgatcgcg gcgaacaaca tcgtcaacgc catcatgatg
     1261 gtggccggtt cggcgttcgt cgccgcatgt tcggcggccg gcctatcggc tcctacggtg
     1321 ctgcggatcg ccgccgtggc gaacttcgcc gtcgcgctat ggatcgttcg cctgctgccg
     1381 cagaccttct tccggacgct gtttcgctgg tatttccgcc tgttccacca aaccagcatc
     1441 gagggcctgg agaatgtcgc cgcccccggc gaacggtcca tcctggtcat caaccatctg
     1501 tctttcctgg acggctgcct ggtcgcggca ttcctgccgg gcgacctggt cttcgcgatc
     1561 gacacggccc aggctcggcg cttctggttt ctgaaatacg tcatcgacgt gttcccgatc
     1621 gacccgaaca gcccgatggc catccgcgca atggtcaaag ccgtgcgtga aggacggcgt
     1681 ctggcggtct ttcccgaagg gcgcatcacg ctgaccggcg cgttgatgaa aatctatgac
     1741 ggtccgggca tggtcgcgga tcgcgccggt gcttcggttg tcgcggtcag gatcgacg
//
LOCUS       DASWQN010000953         5165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2465711, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000953 DASWQN010000000
VERSION     DASWQN010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2465711"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VGC82_16260"
     CDS             <1..355
                     /locus_tag="VGC82_16260"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEY0424873.1"
                     /translation="DNPLVDTPEREERIRQVAFQLWEADGRPDGRDTEFWERASILVR
                     MEESAGAAQLPNPATQDEPIPGVVVEEARIQENYGEFPDRSADQGDWRQTPMTREEMH
                     EFEKGKMQPTRGDAP"
     gene            648..2243
                     /locus_tag="VGC82_16265"
     CDS             648..2243
                     /locus_tag="VGC82_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY0424874.1"
                     /translation="MKISDKAKFGRPETEAVSSVPSPTKLPRPRATTAPVAQLRTVMA
                     GSLLAMVLAALDQNIVNTALPRMVGELGGMTHLSWVVTAFMLTSTTTTPLYGKLSDIY
                     GRRTLFFVAILVFLAGSLLCGAAQSMGQIIAFRAVQGLGAGGLLVLAQAAIGDVVSPR
                     ERPRYQGLFSGAFALSSIAGPLLGGFITSALSWRWVFYVNLPVGLLALIMIAIGLRKP
                     PSGEVRSIDYAGAALLTGATAALLLLLAWGGTEFPWESLDSAGLLALVVILAGLFIRQ
                     ERRAPEPLVRLRLFRNPIFARGVVVSGMMAFAMLGSTVFLPLYFQLVLEMDPATAGMM
                     LLPQVAGMLLTSIIGGRIVTHLGRNKPFLLSGLGLEAVALASLAAFAFTAAPPVAFLV
                     SMAALGLGMGMGMPNLTTAVQNAVAHRELGAATGAMSFVRSLGGALGVATSGTIMSSR
                     LAAAFAGAPGTVDVGALAQHGMQALSHFNPAQQAGVGAAYRTALTGCFLLSAVVMTVA
                     FIVVIGLPELLLRSEVEETAPAE"
     gene            complement(2351..3613)
                     /locus_tag="VGC82_16270"
     CDS             complement(2351..3613)
                     /locus_tag="VGC82_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0424875.1"
                     /translation="MAGLTIHALDRLDMQRLGEARSVAVRVVGLYGTMVLTDPSRRAA
                     VLAELHRGPDLVVTLASSPPEVDLPEMPGAEQRLLRLNMSLIPLGAPQLRWRDLVIYG
                     GHSWNHAVIGMRLSDAEWLNVRVQLEPLQPWHSPMFLAAFLLMTAAAAMMTLWAVRRL
                     TGPVRILAEAAEALGRDVNAPPLPENGPTEVAVAAVAFNTMAARIRRFVQDRTELLTA
                     IGHDLRTPITRLKLRAEFMEDDEQRGKMLSDLEELEAMVSATLAFGRDARATEPVTSL
                     DLVELLRTILDEAGDEHPEIVDKLRYEGPPHLTVRARSLSLKRALVNLIGNAVNYGGS
                     ALVRLSSPVDRTVTVEIEDDGPGIAPGELDRVFEPFHRGEPSRNRETGGVGLGLPIAR
                     NILRAHGGDVVIANRPAGGVRATVTIPV"
     gene            complement(3791..4510)
                     /locus_tag="VGC82_16275"
     CDS             complement(3791..4510)
                     /locus_tag="VGC82_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0424876.1"
                     /translation="MDQNPHILIVDDDREIRELLASFLEKQRIRVTAVRDGREARKAW
                     LNGHYHLVVLDLMLPGESGLDLARWLRGQGEIPIVMLTAMGEETDRIIGLELGADDYV
                     PKPFNPRELLARIRAVLRRSGDQADTKRDPSSRGFRFNNWTLEPARRRLLNPEGVEVA
                     LTGGEYDLLLALLERANRVLTRDMLLDLLRGRQAGPFDRAIDVAVSRLRRKLEDDGRR
                     AQLIKTVRGGGYVLAATVERI"
     gene            4645..5040
                     /locus_tag="VGC82_16280"
     CDS             4645..5040
                     /locus_tag="VGC82_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424877.1"
                     /translation="MRKALLAAATAFVVGGIAAGSVLSQAQPTPPGPPMEHRGMTGRH
                     DGADHGRPFTPGDFALIYRQPDRQLAPADVQKIAEGFLLWNGNHSWKIANVTAAPDGP
                     IGFSLETPEGSMVAKFTMDPHTGRIARVG"
BASE COUNT          875 a         1685 c         1671 g          934 t
ORIGIN      
        1 tgacaatccc ctcgtcgata cgccagaacg cgaggagcgc attcgccaag tggcctttca
       61 actctgggag gcagacgggc ggcccgatgg gcgcgacact gaattttggg agcgagcctc
      121 catccttgtc aggatggagg aaagcgccgg ggcagctcag cttcccaacc cggcgactca
      181 ggacgagccg atcccgggcg ttgtggtgga ggaagcgcgg atccaggaga actatggcga
      241 gttccccgat cgatcggccg atcaaggcga ttggcggcag acgccgatga cccgcgagga
      301 gatgcacgag ttcgaaaagg gtaagatgca gccgacccgc ggcgatgctc cctgagacca
      361 gccatgcggc gatcgcgcat gggtctgctc gacaataacg ccttgggccc cggcttccgg
      421 ttcggttgtg gcagccgcag cctggttcca gtgcttgacc cgatgagttc cctatccagg
      481 ccctcgggag actgcgcacc gctggtcgcg gatggcgagc ccaccgggtc aagcaggccc
      541 tccatccatg gcttgcgatg caccacctgt cgaaggcgtg gatggtgggc gttcgaccat
      601 aagacggtat ttgagatcgt gcccgaagcg gccgcaatgg aaacaacgtg aagatatcag
      661 acaaagccaa atttggccgc ccggaaacgg aagcggtctc gtccgtgccc tcgccgacca
      721 agctgccgag gcctcgtgcc acgacagcac ctgtcgccca actacgaacg gtgatggctg
      781 ggtcgctgtt ggccatggtg ttggcggcgc tggaccaaaa catcgtcaac accgctctgc
      841 cgcggatggt gggcgagctt ggcggcatga cccacctgtc atgggtggta acggcattca
      901 tgctgacatc caccaccacc acgccgcttt acggcaagct ttccgacatt tacgggcggc
      961 ggacgctgtt tttcgtcgcg atcctggtgt ttctcgccgg ctcgctgctg tgcggcgcag
     1021 cgcagtcgat gggccagatc atcgccttcc gagcggtgca ggggctgggt gcgggtggct
     1081 tgctggtgct ggcacaggca gcgatcggcg acgtcgtctc cccgcgagag cggccacgct
     1141 accaggggct gttcagcggc gccttcgccc tgtccagcat agccgggccc ttgctgggcg
     1201 gattcatcac ctccgcgttg tcctggcgct gggtattcta cgtcaatttg ccggtcggcc
     1261 tgctggctct gatcatgatc gcgattggac tgcgcaagcc gccatccggc gaagtgcgct
     1321 ccatcgatta tgccggggcg gcgttgctca ccggggcgac ggcggcgctg ctcctgctgc
     1381 tggcatgggg tggcacggag tttccgtggg aatcgctcga cagcgccggc ctgctggcat
     1441 tggtcgtaat cctggccgga ctgttcatcc gccaggaacg gcgggcgccg gaaccacttg
     1501 ttcgcctccg gctgttccgc aacccgattt tcgcacgcgg cgtcgtagtc agcggcatga
     1561 tggcgttcgc gatgttgggc tccacggttt tcctgccgct ttatttccag ttggtgctgg
     1621 aaatggatcc cgcgacggcc ggaatgatgc tgctgccgca ggtggcgggc atgctgctca
     1681 cctccatcat cggcgggcgc atcgtcaccc atctgggtcg caacaagcct ttcctgctga
     1741 gcgggttggg actggaggca gtcgccctgg ccagcctcgc cgccttcgct ttcaccgctg
     1801 cgccacctgt cgcttttctc gtgtccatgg cggcgctggg gttgggcatg gggatgggga
     1861 tgccaaacct gacgacggct gttcagaacg ccgtcgcgca tcgggaactt ggcgcggcga
     1921 cgggcgccat gagcttcgta cggtcgctcg gcggcgcgct cggcgttgcc acgtcgggca
     1981 cgatcatgtc ctcccggctt gccgccgctt tcgctggcgc accgggaaca gttgatgtgg
     2041 gcgcgcttgc gcagcacggc atgcaggccc tgtcccactt caatccggct cagcaggccg
     2101 gggtcggcgc agcgtaccgg acagcattga caggatgctt cctgttaagc gcagtggtga
     2161 tgacggtggc cttcatcgtg gtaatcggcc tgccggaact gttgctgcgc agcgaggtcg
     2221 aggagacggc gccggccgaa tagaggaatg ctgttgccgc ttcggcgttc cccctcggcc
     2281 ggggacgccg gactgcgctg ccaactttta tccatcgcag gccggatgaa gcccttttcc
     2341 ctgacccaaa tcaaactgga atcgtcacag tcgcccgcac cccgccggct ggacggttcg
     2401 cgatgacaac atctccaccg tgcgcccgca ggatatttcg tgcgatcggc agccccaggc
     2461 ccactccgcc tgtttcccgg ttccggctcg gttctccgcg gtggaagggt tcgaacacgc
     2521 ggtccagttc gcccggggcg atacccggac catcgtcctc gatttccacc gtcacggtcc
     2581 ggtcaaccgg gctcgacagg cgtaccaggg ccgaaccgcc atagttgacg gcgttgccga
     2641 taaggttgac cagcgctcgc ttcagcgaca gcgagcgtgc ccgcacggtc aggtgtggcg
     2701 gcccctcata ccgcagcttg tccacgatct cagggtgctc gtcgcccgcc tcgtccagga
     2761 tcgtgcgcag cagttccacg agatccaggg acgttacggg ctccgtcgcg cgggcatcgc
     2821 gcccaaaagc caacgtggcg gagaccattg cctccagttc ctcgaggtcc gacaacatct
     2881 tgccccgctg ctcatcgtcc tccatgaact ccgcacgcaa tttcaatcgc gtgatcggcg
     2941 tgcgcaggtc gtgtccgatc gcggtcagca gttcggtgcg atcctgaacg aaacgccgga
     3001 ttctggcggc catcgtgttg aacgcgacgg ccgcgacggc aacctcggtc gggccgttct
     3061 ccggcaacgg gggcgcattc acatcccggc ccagcgcctc ggccgcctct gccaggatgc
     3121 ggaccggccc ggtcagccgc cgcaccgccc acagtgtcat catcgccgcg gccgccgtca
     3181 tcaacaggaa cgccgccagg aacattggcg aatgccaggg ctgcagcggt tccagttgca
     3241 ccctcacgtt gagccattcg gcatccgaca gacgcatccc aatgaccgca tgattccagg
     3301 agtggccgcc gtagatgaca aggtcgcgcc aacgaagctg cggcgcaccg agcggaatga
     3361 ggctcatgtt caggcgtagc agccgctgtt ccgcgccggg catttccggc aggtcaacct
     3421 ccggcggcga ggatgccagc gtcaccacca ggtccgggcc gcggtgcaac tccgccagta
     3481 cggcggcgcg gcgcgatgga tcggtcagaa ccatggtgcc gtaaagcccg acgacccgca
     3541 ccgcgacgct gcgtgcctcc cctaaccgct gcatatccag ccggtccagg gcgtgaatgg
     3601 taagcccggc catctgaacg gccgccagtc caagcagaag caccaaggcg gttcgggccg
     3661 cgaggctgcg gggccaaagg cgaactctca taccgacggc cctaaatgtt aacccgtcgc
     3721 tggatcaacg tatgtcggta tcttattgat tcggcgcgca gccgtcacat caatccttcg
     3781 gctgccggta tcatatccgc tccaccgtcg cggcgaggac gtagccgccg ccgcgaacgg
     3841 tctttatgag ctgcgccctg cgtccgtcat cctccagctt gcggcgcagg cgggacaccg
     3901 cgacgtcgat cgcgcggtcg aacgggccgg cctggcgacc gcgcagcagg tccagcagca
     3961 tgtcgcgggt caacacccgg ttcgcccgtt ccagcagtgc cagcagcagg tcgtattcgc
     4021 cccccgtcag cgccacctcg acgccttccg ggttcagcag acggcggcgg gccggctcca
     4081 gggtccagtt gttgaagcgg aaccctcgcg aggatggatc acgtttcgtg tccgcctgat
     4141 caccggagcg gcgcaggacc gctcggattc tggcgagcaa ttcgcgcggg ttgaaaggtt
     4201 tcggcacata atcgtccgcg ccgagttcca gcccgatgat ccggtcggtt tcctcgccca
     4261 tcgccgtcag catcacgatg gggatttcgc cctggccccg cagccagcgc gccaggtcca
     4321 ggcctgactc gcctggcaac atcaggtcga gcacgacaag atggtaatgg ccgttcagcc
     4381 aggcttttcg tgcttccctg ccatcgcgga cggcggtgac gcggatgcgc tgcttctcca
     4441 ggaaactagc cagcaattcg cgaatttcac gatcgtcatc gacgatgagg atgtgagggt
     4501 tctggtccat gaatgtaaca taggctgtca cagacccaag ctggaaacgc cgttgcaatt
     4561 cattgcaatt tgtcgcggga acgagcggtt tcgcaccacc cggatgtgcc agatttcggc
     4621 ggctgcaact cacggaggta ttgaatgcgc aaagccctac tcgccgccgc cacggctttc
     4681 gtcgtcggag gcatcgccgc cggctcggta ttgtcgcagg cccagcccac accgcccggg
     4741 cctccgatgg agcatcgcgg gatgacgggc cggcacgacg gcgccgatca cgggcggcca
     4801 tttaccccgg gcgatttcgc gctgatctat cgccagccgg accgccagtt ggcaccggcc
     4861 gacgtgcaga agatcgctga gggcttcctg ctgtggaacg gcaaccatag ctggaagatc
     4921 gccaacgtca cggcggcgcc ggacgggcca atcggcttca gcctggaaac gcccgagggt
     4981 tcgatggtcg cgaagttcac catggacccg cacactggca ggatcgcccg agtcgggtag
     5041 gggcgtacga aacggggcgt caggtagcga gtgtcggtta gcagggtgtc aattagtggg
     5101 cttgcggcgg ggcgaaggtt gacgccagag tccgccgcat ggagccagac accatggggg
     5161 cggcc
//
LOCUS       DASWQN010000954         4154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1806976, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000954 DASWQN010000000
VERSION     DASWQN010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806976"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            615..1361
                     /locus_tag="VGC82_16285"
     CDS             615..1361
                     /locus_tag="VGC82_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424878.1"
                     /translation="MSNLHSNLQAAIEAPPPVDPATGLMSSVAELLIPLFKTSSIDRQ
                     LARQIAASAIEAYCPETRADFVNVARTIAFSMAALALLGKTASDDLTMPEQMKAFGRA
                     NALNQSADQSERTMMQRRRYLQTTLPAEQPDYEPDVPRVEIGDTEMQAAITEALNEYL
                     AHGGPTGTEVTTSDPAQAAPPLNPIQIPAASSTSAIRYPTPRTEAVQPRTAPPWSASF
                     KSGLLSRSALHNLVGATQGPQKSPQVAPTV"
     gene            complement(1358..2494)
                     /locus_tag="VGC82_16290"
     CDS             complement(1358..2494)
                     /locus_tag="VGC82_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0424879.1"
                     /translation="MDFEPSDDQRLLVESVTRMLSGTYGFAQRKGYMALPEGYSPAVW
                     SQFAEQGLLGLPFAEEYGGFGGSAQEVMLVMQAFGRVLVLEPYFPTVVLAGTALNVAG
                     TAEQKASILPAIAEGSLKMAFAHGERQARYDLTDVVTTARRNGAGWTLDGSKTVVSHG
                     EAADKLIVSARTSGERYDEAGITLFLVDTGSPGVARRGYVSRDDTRAADVSLSNVVVT
                     QADVLGEVGKGLPIIRRVIEAGIAATAGETVGAMEAMNEMTLEYSKTRVQFGNPIGTY
                     QVVQHRLADMFMAQEQGRSMAMLATMSIDNPDDAARARDIALAKVGIGQAGRYVSQSA
                     VQMHGGIGMTEEYAVGHYFRRCMVIERLFGDPAYYLAKLAAEVG"
     gene            complement(2494..3699)
                     /locus_tag="VGC82_16295"
     CDS             complement(2494..3699)
                     /locus_tag="VGC82_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0424880.1"
                     /translation="MDLRFTPEEIAFREEVRTFIRENLPADTRDHMKLGHPPRKEDTV
                     RWQRILNAKGWAALSWPKEWGGPGWTAIQKMIFLEENLMAPAPELLSFNITMLGPVLI
                     QYGTDEQKKRFLPRAANLDDWWCQGFSEPGAGSDLASLKTAAKLDGDHYVVNGQKIWT
                     STAHNADWCFLLVRTDPNARKRQEGISFLLVDMKTPGITVRPIISIDGGHHLNEVFFD
                     DVKVPVEMRIHAENKGWDVAKYLLGNERTGIARLGKSRERVSSAVAMAREVNFGGIPL
                     IQDPTFRQRVAQLQVDMKALEITQYRVVSAYDKAKGAGKPDPLSSVLKVKGTELLQAT
                     SELTVDVAGPMATPIWARELEALNEPDDMLEASSAGTGGYLMLRAASIYGGTNEIQKN
                     ILTKAVLGL"
     gene            3862..3936
                     /locus_tag="VGC82_16300"
     tRNA            3862..3936
                     /locus_tag="VGC82_16300"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3894..3896,aa:Asn,seq:gtt)
     gene            complement(3948..>4154)
                     /locus_tag="VGC82_16305"
     CDS             complement(3948..>4154)
                     /locus_tag="VGC82_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424881.1"
                     /translation="CTSLHRGSIAGDACGLCHFRGTRRIVARYGTGIWSEIRLIEVVG
                     LVATAFVFTSGFIRQAIPPNRMML"
BASE COUNT          782 a         1449 c         1162 g          761 t
ORIGIN      
        1 cctacgcgtc cccgatcaac cgctcattcc gccattgccg caactcgtcc gatgccgcgg
       61 ccagcccgcc tgataccacg gcatgcccgg ggttttgccc ctgttacgtt ttcagcgagc
      121 ccaggcgaac ggaaaacggt ctagcgctgg attgccaaac ttcgcgtgcg aggcgttatg
      181 cttccaccac gccgggggaa ggtgaccgta accggcacgg ccacccatga tgcctgctga
      241 tccaacttcc ttggtgtcga tcaaagcacc cgttccatcg tcccgacctg ccatactctt
      301 agcggtcgct cgcaagccgg ctcaatttcg ctcgcgcagc aggcgggccc ggagccacgc
      361 gcagcccgct ttggcgagca cggcaaggac ccacgtgttg acccggctga gcacgacgcc
      421 aggctcagtc aggaatcgaa cacgccaacc gcccccacgg ttccgcttga aatataacca
      481 gacttgtgag gccgatccgg atcttgccaa catcacccgg aacgagattt ctcttgcaat
      541 aagacaacgt ctgatcgtaa agaacataaa gcgaactgcg aagacggaat ttcagccgag
      601 gccgacccat tcccatgtca aacctgcatt ccaacctgca agcagccatc gaagctccgc
      661 ctccggttga tccggcaacc ggcctgatgt ccagcgtcgc cgaactcctc attccccttt
      721 tcaaaaccag ctcgatcgac cggcagctcg ctcggcaaat agcggccagc gccatcgaag
      781 cctactgccc ggaaacccgt gcggacttcg tcaacgtcgc ccgcaccatc gccttctcca
      841 tggcggcgct cgccttgctg gggaaaaccg catcggatga cctgacgatg ccggagcaaa
      901 tgaaggcctt cggccgggcc aacgccctga accagtccgc cgatcaaagc gagcggacga
      961 tgatgcagcg ccggcgctac ctgcaaacca ccttgccggc cgaacagccg gactacgaac
     1021 ccgacgttcc ccgggtggaa atcggcgata ccgagatgca ggccgcgatc accgaggctt
     1081 tgaacgagta cctggcccac ggcggaccaa ccggcactga agtaaccacg tccgacccgg
     1141 cccaagccgc gccacctctc aacccgatcc aaatacctgc cgcgtcctca accagcgcga
     1201 tccgctaccc aactccccga accgaggccg tccaaccccg aaccgcccca ccctggagcg
     1261 cgtcgttcaa atccggcctt ctgtcgcgga gcgcgctgca caatctggtc ggtgcaaccc
     1321 aaggccccca gaaatcgccg caggtcgcgc cgaccgtcta accgacctcc gccgccaact
     1381 tggccaggta gtaagccggg tcgccgaaca gccgctcgat caccatgcag cggcggaaat
     1441 aatgcccgac cgcgtattcc tccgtcatgc cgatgccgcc gtgcatctgc acggcactct
     1501 gcgacacata ccgcccggcc tgcccaatcc cgaccttcgc cagcgcgata tcccgcgccc
     1561 gcgcggcgtc gtccggattg tcgatcgaca tcgtcgccag catcgccatg ctgcggcctt
     1621 gctcctgcgc catgaacatg tccgccaaac ggtgctgcac cacttggtag gttccgatgg
     1681 ggttgccgaa ctgaacgcga gtcttgctgt attccagcgt catttcgttc atcgcttcca
     1741 tcgcaccgac ggtttcgccg gcggtggccg cgatgccggc ctcgatgacc cgtcgaatga
     1801 tggggagacc cttgccgacc tcacccagca cgtcggcttg cgtaaccaca acattcgaca
     1861 gcgagacatc cgccgcacgc gtgtcgtcgc ggctgacata gccgcgcctc gcaaccccgg
     1921 gcgagccggt atcgaccaga aaaagcgtga tgccggcttc atcgtagcgc tcaccactgg
     1981 tgcgggcact gacgatcagc ttgtccgccg cctcaccatg cgagacgaca gtcttcgatc
     2041 catccagggt ccagccggcg ccgttccgcc tcgcggtcgt cacgacatcc gtcaggtcat
     2101 agcgcgcctg acgttcgccg tgcgcgaatg ccattttcag gctgccttcc gcgatcgccg
     2161 gcaggatcga tgccttctgc tccgccgttc cggcgacgtt cagtgccgtc ccggccagca
     2221 cgacggtcgg gaaatacggt tccagcacca gcacgcggcc gaacgcctgc atgaccagca
     2281 tgacctcctg ggcgctgccg ccaaagccgc catattcctc ggcgaatggc aggcccagca
     2341 gcccctgttc cgcgaactgc gaccagacgg cgggggaata tccctctggt agcgccatgt
     2401 atcccttgcg ctgggcgaaa ccgtacgtgc cgctcagcat gcgcgtcacg ctctcgacca
     2461 gcaggcgctg gtcgtcggat ggttcaaagt ccattacagc cccaataccg ctttggtcag
     2521 gatattcttc tggatctcgt tggtgccgcc atagatggag gccgcgcgca gcatcagata
     2581 gccgccggtt cccgcgctgc tcgcctccag catgtcgtcg ggttcgttca gcgcttcgag
     2641 ttcccgcgcc cagattggcg tcgccatcgg accggccaca tccaccgtca gttccgacgt
     2701 ggcttgcaac agttcggtgc ctttcacctt cagcaccgag gacagcggat cgggcttgcc
     2761 ggcccccttc gccttgtcgt aggccgaaac cacccggtac tgcgtgatct ccagcgcctt
     2821 catatcgacc tgcaactgag cgactcgctg gcggaacgtc gggtcctgga tcaggggaat
     2881 gccgccgaaa ttgacctccc gcgccatggc cacggccgag gagacccgct cgcgcgattt
     2941 acccagccgg gcaatgccgg tgcgttcgtt gccaagcagg tatttggcga catcccagcc
     3001 cttgttctcg gcatggatgc gcatttcgac cggcaccttc acgtcgtcga agaagacttc
     3061 gttcaggtgg tgaccgccgt cgatcgaaat aatcggccgt accgtgatgc cgggtgtctt
     3121 catatcgacc agcaggaacg aaatcccctc ctgccgcttg cgggcgttcg gatcggtccg
     3181 caccagaagg aagcaccaat cggcgttgtg cgcggtcgag gtccagatct tctgtccgtt
     3241 caccacgtaa tggtcgccgt caagcttcgc cgccgtcttc aacgacgcaa ggtccgaccc
     3301 ggctccgggc tccgagaatc cctggcacca ccaatcgtcg aggttggccg ctcgtggcag
     3361 gaagcgtttc ttctgctcat ccgtgccgta ctggatcagc accgggccga gcatggtgat
     3421 attgaaggac agcagctccg gcgcgggcgc catgaggttc tcttccagga agatcatctt
     3481 ctggatcgcc gtccagccgg gtccgcccca ttccttgggc cacgaaagcg ccgcccagcc
     3541 tttggcgttc aggatccgtt gccagcgcac ggtgtcttcc ttgcgcggtg gatgccccaa
     3601 cttcatgtgg tcacgcgtgt ccgcgggcag gttttcacga atgaaagtgc ggacttcctc
     3661 gcggaaggcg atctcttccg gggtgaagcg tagatccatt ggatgtgtcc ctcctactga
     3721 ctggtaactc taggcggcct ggaagggccg cgcaatccgc gccgccggcg ggtcggcgcc
     3781 tggtggattt caacagttga cccgtctgga acggaccact agataggcgt ccctcccggc
     3841 tatcgctgcc gtgaggattg ttcgggcgta gctcagcggt agagcaatcg gctgttaacc
     3901 gattggtcgt aggttcgaat cctaccgccc gagccaccct tccgcgctta aagcatcatc
     3961 cgattaggtg ggatcgcctg ccggataaag ccgctcgtga aaacaaaggc tgtagcgacc
     4021 aatccgacca cctctatcag ccggatttcg ctccagatgc ccgtcccgta gcgcgcaacg
     4081 atgcggcgtg tgccgcgaaa atggcaaagc ccacaggcgt cacctgcgat cgacccccga
     4141 tgcaacgaag tgca
//
LOCUS       DASWQN010000955         4036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1807017, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000955 DASWQN010000000
VERSION     DASWQN010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807017"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VGC82_16310"
     CDS             complement(<1..500)
                     /locus_tag="VGC82_16310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424882.1"
                     /translation="MAGIHLSATEAALEGQRIGPLQLRVAAICLLAQTFDGYDLSSIS
                     MAAPALSQAWHLPGAAFANTFVMSSVGIMVGALSSGPIGDRLGRKPVLLASLLLLAVS
                     SLACVYATTVPVLAALRFVTGLGIGTLMPATVSLTSDYLPERLRATMIMIVFTGAPLG
                     GFLGGQL"
     gene            636..1298
                     /locus_tag="VGC82_16315"
     CDS             636..1298
                     /locus_tag="VGC82_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY0424883.1"
                     /translation="MRITLIHAMAASVPPIEAAFRRLWPEATLMNLLDDSLARDLARD
                     GALTPAMTERFLALARYAKSTGADAILFTCSAFGPCIEACARALAPTPVLKPNEAMID
                     EAVALTGSHGRIGLIATFAPTLTSMPPEFAAAAPGATLVPCLAADALAALNAGDSDGH
                     DAAAVRAAVAMADCDVIALAQFSLSQAAASIAAATGKTVLTTPDSSVRKLRHLLQPAK
                     AA"
     gene            1302..1589
                     /locus_tag="VGC82_16320"
     CDS             1302..1589
                     /locus_tag="VGC82_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424884.1"
                     /translation="MDLAAFQASIPAAAPPDVSLPLQALWWDAKGDWAKAHECAQADP
                     GPAGAAVHAYLHRKEPDLSNARYWYNRAGRTPATGTLQQEWDALAAELLSA"
     gene            complement(1575..3224)
                     /locus_tag="VGC82_16325"
     CDS             complement(1575..3224)
                     /locus_tag="VGC82_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424885.1"
                     /translation="MLNESDFFIATTITVLLIRQRPSAGDLRVNGASRLVLLAFVTCW
                     LVAATVGMASNLGAAYSSNEFLRPDNALRLAKGLLGALVLLPFLRQRQRSHGDAVTLL
                     GWGLAAGLGVVTLVVLAERALFAGILDFAGDYRVAGPFSSMRVGGGHIGAYAALALPF
                     AVCLSRLRPLWLGTGLAMLAFSLGFYTIVITFARTAYAACLVATIATGLGCLFVANRR
                     RIGSLIAGVAPVALIVATLAVAAAFTGMHERFAATATDFVTRQQNWQAGLAVRDRDLP
                     ATMFGMGLGTYQRTMLMRSPVNRPSDLVLERDDLGTYASMRVETPFFLGQKITLPLAG
                     AMHLSLRVRSGDERAVEGQAALGSVICDKVLLYSDNCRGSETKLIEPKTWTSVQVTVP
                     ASGLGSRTLFGLLRRPVELSLYGPVGHRLEVRDIGLTDDSGVSLLANGDFAHGLDRWI
                     FTDDSHVSWRMLNQYLMLFFETGILGLAAFLALAGLAIAGGIQALRGGSPAGAAVAGA
                     VAGFLVSGLSDNILEAPRVATLFFLICCCGLMQWEARSGGQ"
     gene            complement(3494..>4036)
                     /locus_tag="VGC82_16330"
     CDS             complement(3494..>4036)
                     /locus_tag="VGC82_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424886.1"
                     /translation="AVHWLARQDPGLLLQRLRGLVPWVVCPYLVCVLLVNHLFSTHWL
                     SVSQSVAHAYPLGLLPLFDYYIVTKAQAAKNIVGHAVLYMPVGVLLWLRQASPAPGWE
                     GSGRRVGGLAFVMAALLSFLVELARYMRPGLEGDINAVAVAGLASMLAARSMPLVWSM
                     VKALGCQSRYLRPDVGRPSS"
BASE COUNT          812 a         1384 c         1254 g          586 t
ORIGIN      
        1 agttgcccgc cgagaaaacc gccgagggga gcaccggtga agacgatcat gatcatggtg
       61 gcgcgcagcc gttcgggcag gtagtcggac gtcagcgata ctgttgccgg catcaacgtg
      121 ccgatgccaa ggccggtgac gaagcgaagc gcggcgagca cgggcactgt cgtcgcataa
      181 acgcaggcca gcgacgacac cgccaaaagg agaaggctgg cgagcagcac cggcttgcgt
      241 cccagccgat cgccaatggg acccgaagac agcgcgccca ccatgatgcc gacgctggac
      301 atgacgaagg tgttggcgaa ggccgcgccg ggcaggtgcc aagcctgcga cagtgccggc
      361 gccgccatgc tgatgctgct gaggtcatag ccgtcgaagg tctgcgccag taggcagatc
      421 gccgccacgc gaagctgcag cggtccaatg cgctgaccct caagcgcggc ttcggtcgcg
      481 gacagatgta tcccggccat gcctgggtcc tccctgcggc cgcgttgtcc ggcctttgtc
      541 acgatatgac ggatactacc tctgctgccg atacgcgcgt caacagattg cggctgctct
      601 tgtcgtggcc gcatccttgt cgcaccttta cgtccatgcg catcaccctg atccacgcga
      661 tggctgcctc cgtgccaccg atcgaagcgg ccttccgccg cctttggccc gaagcaaccc
      721 tgatgaacct gctggacgat tcgctcgccc gggatctcgc ccgcgatggc gcactgaccc
      781 cggcgatgac ggagcgtttc ctggcgctgg ctcgctatgc caaatccacc ggcgccgacg
      841 ccatcctgtt tacatgctcc gccttcggtc cgtgcatcga ggcctgcgcg cgggctcttg
      901 cgcccacgcc ggtcctaaag ccgaatgagg cgatgatcga cgaagctgtt gccctgaccg
      961 ggtcgcatgg acgcatcggg ctgattgcga catttgcccc aacgctgacc tccatgccgc
     1021 cggagttcgc ggcggcggcg cccggggcaa ctctggtccc gtgtctggcc gcagacgcgc
     1081 tggcggcgct gaatgcaggc gattcggacg gacatgatgc cgcggcggta cgggccgccg
     1141 tcgcgatggc ggattgcgac gtgatcgcct tggcgcagtt cagcctgtcg caggccgccg
     1201 cttcgatcgc cgcggccacc ggcaagacgg tgctgaccac ccccgacagt tccgtgcgca
     1261 agctgcgaca cctgctgcaa ccggcgaagg cggcctgagc catggatctt gccgcgttcc
     1321 aggcgtcaat ccccgctgcc gctccgcccg atgtttcatt gccgctgcag gcgctgtggt
     1381 gggatgccaa aggcgattgg gccaaggccc acgagtgcgc gcaggccgat cccggtccag
     1441 ccggcgccgc ggtgcatgcc tatctgcacc gcaaggaacc agacctgtcc aacgcccgat
     1501 actggtataa ccgagccggc cggaccccgg ccaccggcac gctgcaacag gaatgggacg
     1561 cgctggccgc cgagctactg tccgcctgac ctcgcttccc actgcatcaa gccgcagcag
     1621 cagatcagaa aaaacagggt cgcgacgcgc ggcgcctcca ggatgttgtc ggacagaccg
     1681 gagaccaaga aaccggcgac ggcgccggcc accgccgcgc cggccggcga accgcctcgc
     1741 aacgcctgaa tgccgccggc gatggcaagg cccgccagcg ccaggaaagc cgccagcccc
     1801 aggatgccgg tttcgaaaaa cagcatcagg tactggttca acatacgcca ggacacatgg
     1861 ctgtcgtcgg tgaatatcca gcggtccagg ccatgcgcga agtcgccgtt agccaacagc
     1921 gacaccccgc tgtcgtcggt gagtccgatg tccctgacct cgaggcgatg cccaaccggc
     1981 ccataaagcg ataattccac cgggcgccgc aacaacccga acagcgtccg gctgccaagt
     2041 ccactggccg ggaccgttac ctgcactgaa gtccacgtct tcggttcgat cagtttcgtt
     2101 tccgaaccgc ggcagttgtc cgagtaaagc agcaccttgt cgcagatcac cgaaccaagc
     2161 gccgcctggc cttccactgc ccgctcgtca ccagaacgga ctcgcagcga caggtgcatg
     2221 gcgccagcca gcggcaaagt gatcttttga cccaggaaga atggtgtttc cacccgcatc
     2281 gacgcgtagg tccccagatc atcccgctca agaaccagat cgctcggccg gttcaccggc
     2341 gaacgcatca gcatggtccg ctgataggtg cccaggccca tgccgaacat cgtcgcgggc
     2401 aggtctcggt cccgcacggc cagcccggcc tgccaattct gctgccgcgt cacgaaatcc
     2461 gtcgcagtgg cggcaaaacg ttcgtgcatt ccggtgaacg ccgccgccac agccaaagtg
     2521 gcgacgatca gggccaccgg ggcaactccc gctatgagcg agccgatacg ccgccggttc
     2581 gccacgaaca ggcatccaag gccggtggcg atcgtcgcca cgaggcaagc cgcgtaggca
     2641 gtgcgggcaa atgtaatgac aatcgtatag aaaccgagcg aaaaagcgag cattgccagc
     2701 cctgtcccca gccagagcgg ccgtagccgc gacaggcaaa cggcaaacgg cagcgccagt
     2761 gccgcatagg ctccgatgtg tccgccgccg acgcgcatgc tggaaaacgg ccccgcgacc
     2821 cggtagtcgc cggcaaagtc cagaatcccg gcaaacagcg cgcgttcagc cagcacaacc
     2881 agggtgacga cacccagccc cgccgcgagg ccccaaccca gcaaggtcac ggcgtcgcca
     2941 tggctgcgtt gccgctggcg gaggaacggc agcaggacca gtgcccccag cagtcccttg
     3001 gccaggcgga gcgcgttgtc aggcctcagg aattcgttgc tggaatatgc cgctcccagg
     3061 ttggaggcca ttcccacggt tgccgcgaca agccagcagg tgacgaaggc cagcagcacc
     3121 agacgggatg caccattcac gcgcaggtcg ccggcactag gccgctggcg gatcagcagg
     3181 acggtaatcg tcgtggcgat gaaaaaatcc gactcgttca gcatcgtcca acctgtccac
     3241 agcccgaggt cccaggctgg caggacgatc gggaggatca ggagaaagcc ggccgggtat
     3301 caccagagca cggctgcgta ggcaagcagt gccgtcacga ggggccaggg caacggatat
     3361 cgtgaaacgc caaccatgac ggcgatagcg atcgtaagcg cgatcatccg cgttcctggc
     3421 gagcgaacta ccaggccgac gccgttttcg aacaaggctc gttggccgga ctggctgctt
     3481 ccggaacgca gcctcatgac gagggccgtc caacgtccgg acgaagatac ctcgattggc
     3541 accccagtgc tttcaccatg gaccagacca ggggcatcga cctggctgcc agcatcgagg
     3601 ccaaaccagc gacggcgacc gcgttgatat cgccctccag ccccgggcgc atataccggg
     3661 ccagttccac caggaacgac aacagggccg ccatcacgaa ggccaacccg ccgaccctcc
     3721 gtccgctacc ctcccatccg ggagccggac tcgcctgacg cagccacagc agcacgccga
     3781 ccggcatgta cagtacggcg tggcccacga tgttcttggc ggcctgggcc ttcgtgacga
     3841 tgtaatagtc gaacaacggc agcaggccca acgggtaggc atgcgccacc gattgcgaaa
     3901 ccgacagcca gtgcgtcgag aacagatggt tcaccaacag gacgcacacg agatagggac
     3961 aaaccaccca agggaccaag ccgcgaaggc gttgcagcaa caggccgggg tcctggcgcg
     4021 ccagccagtg gactgc
//
LOCUS       DASWQN010000956         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_619073, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000956 DASWQN010000000
VERSION     DASWQN010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619073"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..1341
                     /locus_tag="VGC82_16335"
     CDS             331..1341
                     /locus_tag="VGC82_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0424887.1"
                     /translation="MLKYFLLAAMIVAGVPVHAATLDAVRQKGFVSCGVNVGLGGFSM
                     PDSKGVWRGLDVDVCRATAAAVFGDAEKVRYVPTTGTSRFAALQSGEIDILARNTTFT
                     FLRDVTIGLRMVMVNFYDGQGFLVRKDAKVSHLADLAGGTICMLQGSTHELNLADWMK
                     QHSTDFHIVLLDTTDVLIKTMLSNRCDAASSDSSNLASIRGAGVPIPDDFVILPDRIS
                     KEPLGPMVRRSDDQWFDIVRWSMMAMLEAEELGLTSANVDKMLAGSPNATAQRLLGKS
                     GEFGKLLGVDNAWAFNIVKQVGNYAESYDRNVGLGSSLKLERGPNALWSNGGLMYPIP
                     FR"
     gene            1338..>1533
                     /locus_tag="VGC82_16340"
     CDS             1338..>1533
                     /locus_tag="VGC82_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424888.1"
                     /translation="MKQSGRNSRSEILAPFAVRSFQFQFPADLLTSWAGEMEILILGW
                     YILTETGSVLLLTVYGSVQYG"
BASE COUNT          263 a          452 c          510 g          308 t
ORIGIN      
        1 tgcgggcatt ccgctccagc agggaccgca cggccatggc gacggcttcg gtcttgtttc
       61 cgccggtcag ccgatcggct gccgactgga tcaaagcgac aacatcaggg cggttgatgg
      121 tgacgacgct cggcatgggg acctcgatca ttataggtat aatgatcgtc cctttgcttg
      181 gtggcttcaa tcgccaccgg aagcgtatcg taaaggtgcg gctcgcttcg gcgccgtcgg
      241 ttgttcgcgg taagggcggc acaagtccct tggtttcttg gctgcgatcg gtaaccatgc
      301 gggcggaata cgaaccgggg tgcatccgca atgctgaaat actttcttct cgccgccatg
      361 atcgtggccg gcgtgccggt tcatgccgcg acgctcgatg cggtccggca gaagggcttt
      421 gtgtcctgcg gcgtcaatgt cggcctcggt gggttctcga tgcccgacag caagggggtg
      481 tggcgaggac tggatgtgga tgtctgccga gcgacggcgg cggcggtatt cggggatgcg
      541 gagaaggtgc ggtatgtgcc gacgacgggc accagccggt ttgccgcgct gcagtcgggc
      601 gagatcgaca tcctggcgcg caacacgacg ttcacctttc tgcgcgacgt gaccatcggg
      661 ctgcggatgg tgatggtgaa cttctacgac ggccaggggt tcctggttcg caaggacgcc
      721 aaggtctcgc atcttgccga cctggcaggc ggcacgatct gcatgctgca gggttcgacg
      781 cacgagctga accttgcgga ctggatgaaa cagcacagca ccgacttcca catcgtcctg
      841 ctggatacga ccgacgttct gatcaagacc atgttgtcga accgctgcga tgccgcgtcg
      901 tccgacagct ccaacctcgc ctcgatccgc ggcgcgggtg tgcccatccc cgacgacttc
      961 gtcatcctgc ccgaccgcat cagcaaggag ccgctgggcc cgatggtccg ccgatccgac
     1021 gatcaatggt tcgatatcgt tcgctggtcg atgatggcga tgctggaagc cgaggagctg
     1081 ggcctgacct cggccaatgt ggacaagatg ctggccggca gtcctaatgc gacggcgcag
     1141 cgcctgctgg ggaaatccgg cgaattcggc aagctgctgg gcgtggacaa cgcctgggcg
     1201 ttcaacatcg tcaagcaggt cggcaattac gccgagtcgt acgatcgcaa tgttggcctg
     1261 ggcagctccc tgaagcttga gcgtggaccc aacgcgctgt ggagcaacgg cggactgatg
     1321 tacccaatcc ccttccggtg aagcagtccg ggcgtaacag ccgctccgag atcctggcgc
     1381 cattcgcggt tcgcagcttt cagtttcagt tccccgccga tctgctgacc tcctgggccg
     1441 gcgagatgga gatcctgatc ctcggctggt acatcctgac cgagactggg tcggtgctgc
     1501 tgctgacggt gtacgggtcg gtgcagtatg gcg
//
LOCUS       DASWQN010000957         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1279484, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000957 DASWQN010000000
VERSION     DASWQN010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279484"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VGC82_16345"
     CDS             complement(<1..445)
                     /locus_tag="VGC82_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0424889.1"
                     /translation="MRLGQRLAQIVPTLILVSVLVFCLQQLMPGDPALVLAGEERGDP
                     RVLAQIRAELWLDRPLPAQYLHWVGNVVRGDLGFSWRTRQPVSQLIAQKLPVTAQLGL
                     MAFVIAILIGVPAGIVAAVKRNGPWDYIANAIGLAGLSMPNFWLGI"
     gene            complement(442..1950)
                     /locus_tag="VGC82_16350"
     CDS             complement(442..1950)
                     /locus_tag="VGC82_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424890.1"
                     /translation="MRFRTLMAFCGFTALAVLPAVAQTLHIALREDPDILDPTLARSY
                     VGRIAFAGLCDKLFDIDEKLQIVPRLALGYEWADPKTLVIHLRPNVTFHDGEAFDADA
                     VKYSLDRHLTMQGSFRRSEIGMIDQVQVVDPLTVRLVLKSPSAPLLALLTDRSGMIVA
                     PKAAEAAGEDFGLHPVCSGPFKFTERVAQDRIVMDRFAGYWDAANIHFDRVIYQPMPD
                     SAVLVANLHTGNVDLAERVLPTDVAEIQKDAKLRIVTSPALGYEGITFNLGNGDRSKG
                     PIGQNVLLRQAFDAAIDRQALVEVVFNGMYKPTVQAVPASSPFYVPNLEPQARDIARA
                     QALVKQSGVPTPIAVTMNVPNTADRTQLAEVIQSMVRDAGFDLKLNLFEFASSLSAAN
                     QGNFETYLIGWSGRTDADGNLYAFLHTGAGQNDGRYTNPVVDAALDAARTLPDPAARR
                     TEYGRMMEQERKDLPIIYLFHPVNVVGLSAKLSGFRSVPDGLIRLQGLSVAR"
     gene            2113..>3034
                     /locus_tag="VGC82_16355"
     CDS             2113..>3034
                     /locus_tag="VGC82_16355"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY0424891.1"
                     /translation="MTIRRFATLAAALLLAAAPAMAQDKTVTLKMSSWVPPAHPLNPS
                     LIAWAADIEKESAGTIKATLFPSEQLGKAFDHYDMARDGITDLAYVNPGYQPGRFPII
                     AAGELPFLIANAKGGSAAFDDWYRPYAAKEMKDTHFCFAFLLDPEAFHSRRKILLPND
                     LKGDKVRPGDATQGAFVTLLGGTNVQASAPEAREVLERGVANTIIFPWGSISLFGIDK
                     VLKYDMDEPMFSTTFVWTMNQAKYDSLSAAQRTVIDHHCTPEWAEKVATPFADFEHAG
                     HAKLAAEAGHDAYTLTPDQRAEWRKAAEPLT"
BASE COUNT          586 a         1000 c          938 g          510 t
ORIGIN      
        1 tgatccccag ccagaaattc ggcatcgaca atccagcgag cccgatcgcg ttggcgatgt
       61 agtcccacgg cccgttccgc ttaaccgccg cgacgatccc ggccggcacg ccaatcagga
      121 tggcgatcac gaacgccatc aagcccaact gagcggtcac cggcaacttt tgcgcaatca
      181 actgcgacac cggctggcgc gtccgccagg aaaaacccag gtcacctcgc accacgttgc
      241 cgacccagtg caggtactgc gccggcaacg gccggtcgag ccaaagctcc gcccgtatct
      301 gcgccaggac gcgcggatcc cccctttctt caccggccag cacgagggcc gggtcaccgg
      361 gcataagctg ctgcaagcag aacaccagca cgctgaccag tatcagggtc ggaacgatct
      421 gcgcgagccg ttgcccgagc ctcaccgggc caccgagagg ccttgcaagc ggatcagccc
      481 atcgggcacc gaacggaaac cggatagttt tgccgataag ccaaccacgt tcaccggatg
      541 gaacaggtag atgatcggca ggtccttgcg ctcctgctcc atcatccggc cgtactccgt
      601 tcggcgtgcg gctggatcgg gcaaggtacg ggccgcgtcg agtgccgcgt caaccaccgg
      661 attcgtgtag cggccgtcgt tctgccctgc tccggtgtgc aggaaggcat acagattgcc
      721 gtccgcatcc gtccggccgg accagccgat cagataggtt tcgaagttgc cctggttggc
      781 ggcgctcaaa gacgacgcga actcgaacaa attcaacttc aggtcgaagc cggcgtcacg
      841 caccatcgac tggataactt cggccagctg ggtgcggtcg gcggtgttcg gcacgttcat
      901 ggtaaccgcg atcggcgtcg ggacgcccga ctgcttcacc agcgcctgcg cacgcgcgat
      961 atcccgcgcc tgaggctcca ggtttggcac gtagaacgga gaggacgccg ggaccgcctg
     1021 cacggtcggc ttgtacatgc cgttgaacac cacctcgacc agcgcctgac ggtcgatcgc
     1081 ggcatcgaac gcctggcgaa gcagtacatt ctggccgatc ggtcccttcg agcggtcgcc
     1141 attgccgagg ttgaaggtga tcccttcgta gcccagcgcc ggcgaggtca cgatgcgcag
     1201 cttggcgtcc ttctggattt cggccacgtc ggtcggcagt acccgttcgg cgagatcgac
     1261 gttgccggtg tgcagattgg ccaccagcac cgcgctgtcc ggcatcggct ggtagatcac
     1321 ccggtcgaaa tggatatttg ccgcatccca gtagccggcg aagcgatcca tcacgatccg
     1381 gtcctgcgcc actcgttcgg taaacttgaa gggaccgctg cacaccggat gcaggccgaa
     1441 atcctcgccg gcggcctccg ccgccttggg agcgacaatc attccggagc ggtcggtcag
     1501 cagtgccagc aacggcgccg atggcgattt cagcaccagc cgcacggtca gcggatcgac
     1561 aacctgcacc tggtcgatca tgccgatctc tgaacgccgg aagctgcctt gcatcgtcag
     1621 gtgccggtca agactatact tcaccgcatc ggcgtcgaaa gcttcgccgt cgtggaaggt
     1681 gacgttcggc cgtaggtgga tcaccagcgt ctttggatcc gcccattcgt aaccaagggc
     1741 gagccgcggg acaatttgca gcttctcatc gatgtcgaac agcttgtcgc acaagccggc
     1801 gaaggcgatg cggccgacat aagacctggc gagcgtcgga tcgagaatgt ccggatcctc
     1861 gcgcagggcg atatgcagcg tctgtgcgac ggctggcagc acggcaaggg ccgtgaaacc
     1921 gcaaaacgcc atcagcgtac gaaatcgcat tcgccggctc cctgccattc cttcaagccg
     1981 attgtgcggg cgaccggggc tggctgcaag gcgcggcgcg ccccgtggga tgccggtagc
     2041 caacagcctg ccgggcaagc tatcagcatc gccacaacgg ccggcgcgac cggcgaacgg
     2101 gagacaacgt gcatgaccat cagacgcttt gcgactcttg ccgccgctct gctgctggcg
     2161 gccgcgcccg ccatggcgca agacaagacg gtcacgctga aaatgtcgtc gtgggtcccg
     2221 ccggcgcacc cgctgaatcc gtcgctgatt gcctgggcgg cggatatcga gaaggagtcg
     2281 gccggcacca tcaaggccac gctgttcccc tccgaacaac tcgggaaggc attcgaccac
     2341 tatgacatgg cgcgcgatgg catcaccgat ctggcctatg tcaatcccgg ctaccagccc
     2401 ggccggttcc cgatcatcgc agccggcgag ctgccgttcc tgatcgccaa cgccaagggc
     2461 gggtcggccg cgttcgacga ctggtaccgt ccctatgcag cgaaggaaat gaaggacacg
     2521 catttctgct tcgccttcct gctggaccct gaggcgttcc attcccgccg caaaattttg
     2581 cttccgaacg acctcaaggg cgacaaggtg cgcccgggcg acgcaacgca gggcgccttc
     2641 gtgaccttgc tcggcggcac caacgtgcag gcatcggccc ccgaggcgcg cgaggtgctg
     2701 gagcgcggcg tcgccaacac gatcatcttc ccctggggat cgatctcgct gttcggtatc
     2761 gacaaggtgc tgaaatacga catggacgag ccgatgtttt cgacgacatt tgtctggacg
     2821 atgaaccagg cgaagtatga ctcgctgtcg gcggcgcagc ggacggtgat cgatcatcac
     2881 tgcacgcccg aatgggcaga gaaggtcgcc accccgttcg cggatttcga acatgccggt
     2941 catgccaagc tggccgccga agccggccac gacgcttaca cgctgacgcc ggatcaacgt
     3001 gcggaatggc gcaaggcagc ggaaccgctg acgg
//
LOCUS       DASWQN010000958         5389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2599658, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000958 DASWQN010000000
VERSION     DASWQN010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2599658"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1686
                     /gene="fusA"
                     /locus_tag="VGC82_16360"
     CDS             <1..1686
                     /gene="fusA"
                     /locus_tag="VGC82_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY0424892.1"
                     /translation="KLDRTGADFFRAFATLKEKLDIVALPLQLPVGTEDQFLGVVDLV
                     EMKAIIWEGGELGAKYHYEDIPADMTELVATHRQTLLDTALSVDEKAMEEYFDKGDVS
                     IETMKRCIKRGTIDGTFRPVLCGTAFKNKGVQPLLDAVIDYLPSPIDVPGIAVAVEEG
                     DDEHDIANRRRIPADPKAPFAGLAFKIINDKYGTLTFVRVYAGTLKSGDMVQNTTKGH
                     RERIGRMFQMHADKRAEIKEVEAGDIAAFVGLKDTGTGDTLASNDDPVVLERMAFPVP
                     VIDISVEPRTKEAVEKMTLGLQKLAGEDPSLRLRTDQETGQTILSGMGELHLEIIIDR
                     LRREYGVDCNIGAPQVAYRETISKSHAHIYTHKKQSGGSGQYAEVKIVFEPLERNTGV
                     VFENKVVGGSVPREYIPAVEKGIKVQADTGVLAGFPTVDFKYTLVDGKYHDVDSSALA
                     FEIAAKACFREGMKEAGPIILEPIMDVEVTTPQDHVGDVVGDLNRRRGQIQNQESSGS
                     TVIVRAHVPLKEMFGYISHLRSMTKGRASFTMQFHHYDPVPRNVADEIMKKSA"
     gene            complement(1800..2477)
                     /gene="dnaQ"
                     /locus_tag="VGC82_16365"
     CDS             complement(1800..2477)
                     /gene="dnaQ"
                     /locus_tag="VGC82_16365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEY0424893.1"
                     /translation="MTRAVLFDTETTGLDPLNGDRVIEVAALELINELPTGKHFHALI
                     DPQRDIPPEASRVHGFTSGDLAGKPLFASIAQPLMEFFGDAKLIAHNAPFDFSFLNME
                     FGRAGLPGLARDRMIDSLALAKIRFPGMPNSLDALCRRFSIDLSARTTHNALLDCKLL
                     AEVYVELTGGRQRGLSLAAQDARGPVAVYFHTGPRTPHPIVPTAAELAAHAAFLTRIK
                     EPVWLLP"
     gene            complement(2474..3061)
                     /gene="coaE"
                     /locus_tag="VGC82_16370"
     CDS             complement(2474..3061)
                     /gene="coaE"
                     /locus_tag="VGC82_16370"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227202.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY0424894.1"
                     /translation="MRVIGLTGGIGMGKSTAAAAFRRARIPVFDADLAVHRLQAKGGK
                     AVRAIEAAFPGTMRDGATDRVALRQAVLGNPQALTKLEHILHPMVEAAERDFVARARR
                     AGRHAVVLDIPLLFEVKGDERVDTIVVVSAPLAIQIHRVGLRRRMSKADIAAIIARQM
                     PDREKRRRADLVVKTGLSRNHTLRILTRFIKGLRR"
     gene            complement(3072..3911)
                     /locus_tag="VGC82_16375"
     CDS             complement(3072..3911)
                     /locus_tag="VGC82_16375"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY0424895.1"
                     /translation="MTILTGTARLAGITGWPVSHSRSPRLHGFWLDRYGIDGAYLPLP
                     IEPARFPEAVRGLMLSGFAGINCTIPHKIAAFEVCDVVDPSARRAGAVNTLVFEGGRI
                     IGSNTDGWGFLENLRADGINPAAGPALVLGAGGSARAVSAVLLDLGVKVTVANRTRTR
                     AEGLARELPGLAVIDWDRRDLALADQALLVNTTSLGMSGHPPLEIDLDRGATGLTVAD
                     IVYVPLETPLLAAARVRQIRCVGGLGMLLYQAVPGFRTWFGVEPSVDEGLRRFVAADL
                     LTA"
     gene            complement(4031..4888)
                     /locus_tag="VGC82_16380"
     CDS             complement(4031..4888)
                     /locus_tag="VGC82_16380"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEY0424896.1"
                     /translation="MHWTDRRERYRETIAGSACIHPGSVYDAISARIAEELGFEVGMF
                     AGSIASFAVLGAPDLIVLTLTEFAAQAYRINRAGKLPLMVDADHGYGNALNVMRTVQE
                     LETAGVAGMMIEDTLLPRAFGEKKPAVISIEEGIGKMRAALAAREDKALTIIGRTSSP
                     SISGLADGIKRLKAYEATGVDALFIAGGLTRSDLDAIAGEVKIPLMLGGVSGDLQDKA
                     YLASRGVRIALQGHQPFSAAVKATYDTLKALRDGTKPSALTGIASSEMMARVTRDGDY
                     KSWTEKFLS"
     gene            5195..>5389
                     /locus_tag="VGC82_16385"
     CDS             5195..>5389
                     /locus_tag="VGC82_16385"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0424897.1"
                     /translation="MQLAIPVSALLPLIPLSARAVLHVGCGSGELAAAYRQWNPKARL
                     LGIEPDAVAASRAAAHMHQVS"
BASE COUNT         1017 a         1736 c         1724 g          912 t
ORIGIN      
        1 aagctggacc gcaccggtgc cgacttcttt cgcgccttcg ccacactgaa ggaaaagctg
       61 gacatagtcg ctctgccgct gcaactgcca gtaggcaccg aggatcagtt cctcggtgtc
      121 gtcgacctgg tcgagatgaa ggcaatcatc tgggaaggcg gcgagctcgg cgcgaagtat
      181 cactatgagg atattccggc cgacatgacc gagctggtgg cgacgcaccg ccagaccctg
      241 ctcgataccg cgctgtcggt cgatgagaag gcgatggagg agtatttcga caagggcgac
      301 gtctccatcg agaccatgaa gcgctgcatc aagcgtggca cgatcgacgg caccttccgt
      361 ccagtgctgt gcggcacggc attcaagaac aagggcgtgc agccgttgtt ggacgcggtg
      421 atcgactacc tgccatcccc catcgacgtg cccggcatcg ccgtcgcggt cgaggaaggc
      481 gatgacgagc acgacatcgc caaccggcgg cggatcccgg cagacccgaa ggcgccgttc
      541 gccggcctgg cgttcaagat catcaacgac aagtacggca ccctgacgtt cgtgcgggtt
      601 tatgccggca cgctgaagtc gggggacatg gtgcagaaca ccaccaaggg ccaccgcgag
      661 cgcatcggcc ggatgttcca gatgcacgcc gacaagcgtg cggagatcaa ggaagtcgaa
      721 gccggcgaca tcgccgcctt cgtcggcctg aaggacaccg gcaccggcga tacgctggct
      781 tccaacgacg atccggtcgt actggagcgg atggctttcc cggtcccggt gatcgacatc
      841 tcggtcgagc cgcgcaccaa ggaagccgtc gagaagatga ctctgggcct gcaaaaactg
      901 gccggcgagg atccgtcttt gcgccttcgc accgaccagg agactgggca gaccattctt
      961 tcgggcatgg gcgaactgca cctggagatc atcatcgacc gtctgcgccg ggagtacggc
     1021 gtggattgca atatcggcgc gccgcaggtg gcatatcgcg aaacgatctc gaagtcacac
     1081 gcgcatatct atacccacaa gaagcaatcc ggcggctcgg gccagtacgc cgaagtaaag
     1141 atcgtcttcg agccgctgga gcggaacacc ggcgtcgtct tcgaaaacaa agtggtcggc
     1201 gggtcggtgc cgcgggaata cattccggcg gtggaaaagg ggatcaaggt ccaggccgat
     1261 accggcgtgc tggccggctt cccgacggtg gacttcaaat acaccctggt ggatggcaag
     1321 taccatgatg tcgattcctc ggcactggcg ttcgaaattg ctgccaaggc ctgtttccgc
     1381 gaaggtatga aagaggccgg cccgatcatc ctggagccga tcatggatgt ggaagtcacc
     1441 acgccgcagg atcatgtggg cgacgtcgta ggcgacctca atcgccgtcg tgggcagatc
     1501 cagaatcagg aaagctccgg ctccaccgtg atcgtgcggg cgcatgtgcc gctaaaggag
     1561 atgttcggct atatctcgca cctgcgcagc atgacgaagg gccgtgcatc cttcaccatg
     1621 cagttccacc actatgaccc ggtgccgcgc aacgtcgccg acgagatcat gaaaaagagc
     1681 gcctgaggcg ggcctgcagg ctgactgaag gcggcgcgaa ccatctccgg ttcgcgccgc
     1741 cttttttatg agggaggcca gcatcgattg ccggtcagac cgcgaacgtc gtcagataat
     1801 cacggcagca gccagaccgg ctccttgatc cgggtcagga acgccgcatg agccgccaat
     1861 tccgccgcgg tcggcacgat cggatgtggc gtccggggtc cggtgtggaa atacaccgca
     1921 acaggtccac gtgcgtcctg cgcggcaagc gaaagcccgc gctgtcggcc gccggtcagt
     1981 tcgacataga cctcggccag cagcttgcag tccagcagcg cgttgtgcgt ggtgcgcgcc
     2041 gacagatcga tggagaaccg ccggcacagc gcgtccaggc tgttcggcat gcccggaaag
     2101 cggatcttcg ccagcgccaa ggagtcgatc atccgatccc gcgccagccc gggcaaaccc
     2161 gctcggccga attccatgtt caggaaactg aaatcgaacg gcgcattgtg ggcgatcagc
     2221 ttggcgtccc cgaagaactc catgagtggc tgcgcgatcg aggcgaacaa cggctttccc
     2281 gcaaggtcgc cgctggtgaa accgtggacg cgtgaagcct cgggcggaat atcgcgctgc
     2341 gggtctatca acgcgtggaa atgcttgccg gtgggcagtt cgttgatcaa ttccagcgcc
     2401 gcgacctcga ttacccggtc cccgttcagc gggtccaggc cagtggtttc ggtatcgaac
     2461 agcacagcgc gggtcatctg cgaagaccct tgatgaaccg cgtgaggatt cgcaaggtat
     2521 gattgcgcga cagcccggtt tttaccacca gatcggctcg gcgccgcttt tcccgatcag
     2581 gcatctggcg cgcgatgatg gccgcgatgt cggccttgct catgcgccgg cgcagcccga
     2641 cacgatggat ctggatcgcc agcggcgcgg acaccaccac gatcgtatcg actcgttcgt
     2701 cgcccttgac ctcgaacagc agcgggatgt ccagcaccac cgcatgtcgg ccggcgcgcc
     2761 ttgcgcgggc gacgaaatcg cgttcggcgg cttcgaccat tgggtgcagg atatgctcca
     2821 gcttcgtcag cgcctgcggg ttgcccagca ccgcctgacg cagcgcaacc cggtccgtgg
     2881 cgccgtcgcg catggtgccg gggaaggcgg cctcgatggc acgcaccgcc ttgccacctt
     2941 tcgcctgtaa ccggtgcacc gcgaggtcgg catcgaagac tggaatccga gcgcggcgga
     3001 acgcagcggc ggcggtggat ttacccatgc cgatgccgcc ggtcaggccg atgaccctca
     3061 agctggtact ctcacgccgt cagcaggtcg gcggcgacaa accggcgcag accctcgtcc
     3121 acactgggct ccacgccaaa ccatgtccga aaccctggga cggcctgata cagcagcatc
     3181 cccagccccc cgacacagcg gatttgccga acccgcgcgg cggccagcag cggcgtttcc
     3241 agtggcacat aaacaatgtc ggcaaccgtc aggccggttg cgccacgatc gagatcgatc
     3301 tcgagcggcg ggtgtccgga catccccagc gaggtcgtgt tgaccagcag cgcctgatcc
     3361 gccagggcca aatcccgccg atcccagtcg atcaccgcca gcccgggcag ttcgcgggca
     3421 agcccctcgg cgcgcgtgcg ggtgcggtta gcgaccgtca ccttgacccc aagatccagg
     3481 agcaccgcgc tgacggcgcg ggcggaacca cccgcaccca ggaccagcgc cggaccggct
     3541 gccggattga tcccgtcggc ccgaaggttc tccaggaagc cccaaccgtc ggtgttgctg
     3601 ccgatgatac ggccgccttc aaacaccagc gtgttgaccg cgccggcgcg gcgggctgac
     3661 gggtccacga cgtcgcaaac ctcgaacgcc gcgattttgt gcggaatggt acagtttatg
     3721 ccggcgaacc cggacagcat caggccgcgc accgcctccg gaaaccgggc cggctcgatc
     3781 ggcagcggca aataggcgcc gtcgatgcca tagcggtcca gccagaagcc atgcaggcgc
     3841 ggggagcgcg agtggctgac aggccagccc gtgatgcccg caagccgagc cgtgccagtg
     3901 agaatcgtca tgcagccacc gtttcgcgcg ggcagcagcc ccggattatt tgttcggccc
     3961 cagcaggaat ccgccaagcg gtgggcggcc ggtacgtttc cggccgcgag acaaggtcag
     4021 gggtcaccgg tcagctcagg aacttctcgg tccagctttt atagtcgccg tcccgggtta
     4081 cccgcgccat catctcggac gaggcgatcc ccgtcagggc cgatggcttc gttccgtccc
     4141 gcaatgcttt cagcgtgtca tacgtcgcct tgaccgccgc agaaaacggc tggtggccct
     4201 gcagcgcgat gcgtacgccg cggctggcca aatacgcttt gtcctgcagg tcacccgaaa
     4261 ccccacccag catcaacggg atcttgacct ccccggcgat ggcatcgaga tcggaccggg
     4321 tgaggccgcc ggcgatgaac agcgcgtcca cgcccgtcgc ctcgtaagcc ttcaggcgtt
     4381 tgatgccatc ggccaggccg ctgatcgacg gtgaactggt ccggccgatg atggtgagcg
     4441 ccttgtcctc gcgcgccgct agggccgcac gcatcttgcc aatcccttcc tcgatggaga
     4501 tcaccgccgg cttcttctcg ccgaacgcgc gcggcagcag cgtgtcctcg atcatcatgc
     4561 cggcgacgcc tgcggtctcg agctcctgga ccgtgcgcat cacgttcagc gcgttgccgt
     4621 agccatgatc ggcatccacc atcagcggca gtttcccggc gcggttgatg cggtaggcct
     4681 gggcggcgaa ttccgtcagt gtcagcacga tcaggtcggg cgcaccgagc acggcgaagg
     4741 aggcgatgga gccggcgaac atgccgactt cgaagccgag ttcctccgcg atgcgggccg
     4801 agatagcgtc ataaacggag ccgggatgga tgcaggcgct gcccgcgatg gtttcgcgat
     4861 agcgttcgcg gcgatcggtc cagtgcatga tttcccccgt ttcgaagtgg ccgcatgtta
     4921 ggggcgtcgc ggccattggg gaagggagac tgccgcgaaa gcatcaagcc agaagagcat
     4981 gtgcggctgc attgcgttgc agcggttacg tgcgacgcct tgaagcttca gcacggttgg
     5041 cggcctcagg atcacgatcc ttgcatatcg gctcccattc cgcctaccag ctaacatcag
     5101 tgggctgacc gcggagcatc gcggccggaa attgctccgg cgaccagtcg aacaggctcc
     5161 gccattttac gtttcgcgtt atttacgggt gccgatgcag cttgctattc cagtgtccgc
     5221 cctgctgccg ctgattccgc tatcggcgcg tgcggtactg catgtcggat gcggcagtgg
     5281 tgaacttgcc gcagcatatc gccagtggaa cccgaaagcg cgcctgctgg gcatcgaacc
     5341 cgacgcggtc gctgcttcac gggccgcggc gcacatgcat caggtatcg
//
LOCUS       DASWQN010000959         4385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2466260, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000959 DASWQN010000000
VERSION     DASWQN010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2466260"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VGC82_16390"
     CDS             <1..252
                     /locus_tag="VGC82_16390"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0424898.1"
                     /translation="LAEQAGMSERSFLRHYRQITGTTPARAVERVRVEAARKALGETR
                     LPVKRIARDCGFGSEETMRRSFMRVLAVLPHAYRQRFPA"
     gene            complement(369..1397)
                     /gene="pdxA"
                     /locus_tag="VGC82_16395"
     CDS             complement(369..1397)
                     /gene="pdxA"
                     /locus_tag="VGC82_16395"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY0424899.1"
                     /translation="MNPLAITMGDPAGVGPEIIVKAAAELFPRIQSGALRMVVIGSNP
                     ALELVRKRFAPELVIPTVTADDRNWPALCALQAGPEGEPIRPGVLSAEGGRFAFQAVE
                     LGVRLALDGRIGGIVTAPLNKEALNKAGYHYPGHTEMLAELTGVKGSCMMLAHGNMRV
                     SHVTTHIALEDVPKRATAERLRRVIDLTHDALVRLGLGRTRIAVAALNPHAGEGGLFG
                     RHDIDVAEPVIAKAVAEGLDVVGPVPGDTVFVKLRAGQYDAVIANYHDQGHIPVKLLG
                     FEIDPATGRWDALSGVNITLGLPIIRTSVDHGTAFDIAGQGIANARSMIEAIEYAERL
                     AAHGNRIR"
     gene            complement(1394..2464)
                     /locus_tag="VGC82_16400"
     CDS             complement(1394..2464)
                     /locus_tag="VGC82_16400"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEY0424900.1"
                     /translation="MPTLRLLADDLTGALDTAGEFVPMTGALPVFWANGNRLNLPVSA
                     AVDSGTRECDPAVAMARVGALVHVLEGADIAFKKIDSLLRGPTIAEIVACMRAGIWRH
                     CALAPAFPFQGRVTRGGRQYAGMNAVSDELVAVLQSFGLAAQPGRADGVLQPGISAFD
                     AETDLDLQRIVATVRHCAEPVLWVGTGGLAQALTEGHDHPAAPKLPRPVLGLFGSDQK
                     VSADQLAACEPHWLQIEDHEFDVVRLRLGDTGLALISFRLPNGVPRTLAASRIASGIA
                     RLVDRIDPPGAVVVAGGETLRSLCDALGTTSLEVQGRLMPGVPRSIMIGGRWNGVTVV
                     SKSGAFGHSSLLRELILERTAP"
     gene            2556..4142
                     /locus_tag="VGC82_16405"
     CDS             2556..4142
                     /locus_tag="VGC82_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0424901.1"
                     /translation="MQRRSFLAGTAAATAVSRKVMAQTTKTRILRYVPQADLTVIDPV
                     MTTAYITRYHATMVWDQLYGIDSKLQPQPQMVAGHTVEDDGRLWTFTLRDNLRFHDGE
                     PVRGRDCVASIKRWAARDPMGQALLARVAEMGAPDDRRFTIRLTRPFSLVLNCLSKIG
                     PPTLVVMPERLANTDPFQSIKDVVGSGPYRFVASERVVGSKVVYVRNADYVPRPDGTP
                     DWLAGPKIVNFDRVEWTVMPDPATAAAALKNGEVDWWENPPNDLLPMLNRSADVRTEA
                     TGPLGTIGTGVFNHLYPPFDKPAVRRVLLQAISQADFMTAAAGSDPEMWRDGIGIFTP
                     GTPMANTAGLDIVTSPKPSLPNLAKALREAGYQNERIILMSPSDQAVLTAQCAVAVDL
                     MQKLGMQVDLQMMDWGTLVQRRASKEPPAKGGWNMFITGWSGLDMTTPITNQTLRSNG
                     AKAWFGWPDLPQVQSLIDAWVEAPDTAAQQKIAVEIQAKAMEQVPYLPTGQYFYRTAC
                     RKDITGIIPGQFVFWNARRT"
BASE COUNT          809 a         1484 c         1405 g          687 t
ORIGIN      
        1 ctggcggaac aggcgggcat gagcgagcgc agtttcctgc ggcactatcg gcagatcacc
       61 gggacgacgc ctgcccgagc cgttgagcgc gtacgggtgg aggcagcgcg caaggcgctt
      121 ggcgaaacgc gcttgccggt aaagcggatc gcacgcgatt gcgggttcgg gtcggaggaa
      181 accatgcggc gtagtttcat gcgcgttctg gcggttctcc cgcatgccta tcggcaacga
      241 ttcccggctt gaagcccccg gtccggggaa caaggtgcat aggttccaga cagaaaaatg
      301 caccagccga gctaaggcgt cgccgccgca atcagcggat ctcttcgcct gcttgcctcg
      361 gggcactcct accggatgcg gttcccgtgg gcagccaggc gttccgcata ctcgatcgcc
      421 tcgatcatgc tgcgggcgtt ggcgatcccc tggccggcga tgtcgaacgc cgtcccatga
      481 tccaccgagg tgcggataat cggcagcccc agagtgatgt tcacgcccga aagagcgtcc
      541 cagcgtccgg tcgccgggtc gatctcgaaa cccagcagct tgaccggaat gtgtccctgg
      601 tcgtggtagt tggcgatgac ggcgtcatac tgtccggcgc gcaacttgac gaacacggtg
      661 tcgccgggga cggggccgac gacatccaga ccctcggcga cggccttggc gataacgggt
      721 tccgcgacat cgatgtcgtg acggccgaac aatccgccct cgccggcatg cgggttcaat
      781 gcggcgacgg cgatccgcgt ccggcccagg cccaggcgga ccagcgcatc atgcgtcagg
      841 tcgatcacgc gccgcaggcg ttcggccgtg gcgcgcttcg gcacgtcctc cagcgcgata
      901 tgggtggtga cgtggctgac gcgcatgttg ccgtgcgcca gcatcatgca ggagcctttg
      961 acgccggtca gctcagccag catctcggta tgtccgggat agtggtagcc ggccttgttc
     1021 agcgcctcct tgttcaacgg cgcggtcacg atgccgccga tgcggccgtc gagcgccagg
     1081 cggacgccaa gctccacggc ctggaaggcg aaacgcccgc cctcggctga caacacgccc
     1141 ggacggatcg gttcgccctc gggaccggcc tgcaaggcgc acagcgccgg ccagttccga
     1201 tcgtccgccg tcaccgtggg aataacgagc tccggcgcga accgcttccg aaccagctcc
     1261 agggctggat tgctgccgat gacgaccatc ctcagcgctc cgctttggat ccgcggaaac
     1321 agctcggcag cggccttgac gataatttcc gggccgacgc cggcaggatc gcccatggtg
     1381 atcgcgagag ggttcatggt gccgtccttt caaggatcag ttcgcgcagc aacgaggaat
     1441 gtccgaacgc ccctgacttg gagacgacgg tgacgccgtt ccaccgtccg ccgatcatga
     1501 ttgagcgggg aacgccgggc atcaagcggc cctgtacttc caggctagtg gtcccgagcg
     1561 catcgcacag gctgcgcagg gtttcgccgc cggcgacgac gacggcaccc ggcggatcga
     1621 tccgatcgac gaggcgcgcg atcccactcg cgatacgcga agcagccaaa gttcggggaa
     1681 caccgttcgg cagccggaaa ctgatcaaag ctaacccggt gtcaccaagg cggagccgaa
     1741 caacatcgaa ttcgtggtcc tcgatctgca accaatgcgg ctcacaggcg gcaagctggt
     1801 cggcgctcac cttctgatcc gaaccgaaca gcccaagaac aggccgaggc agcttcggcg
     1861 cggccggatg gtcgtggccc tccgtcagcg cctgcgcaag cccgccggtg ccaacccaaa
     1921 gcacgggttc ggcacagtgg cggaccgtcg cgacgatccg ctgcagatcc aggtccgttt
     1981 cggcatcgaa cgcgctgatc ccgggctgca gcacgccgtc cgcgcgtccc ggttgcgccg
     2041 ccagcccaaa cgactgcaac accgccacaa gctcatcgct gacggcgttc ataccggcat
     2101 actgcctgcc gccacgggtc acgcggccct gaaacggaaa tgccggagcc agcgcgcaat
     2161 gccgccagat cccggcccgc atgcacgcga cgatttcggc gatcgtcggc ccgcgcagca
     2221 ggctgtcgat tttcttgaaa gcgatgtcgg caccttccag gacgtgaacc agcgcgccga
     2281 cccgggccat cgccacggcg ggatcgcact ctcttgtgcc gctgtccacg gctgcgctga
     2341 ccggcaggtt caagcgattc ccattggccc agaagaccgg caaagcgccc gtcatcggga
     2401 caaactcgcc cgccgtatcc agcgcgccgg tcaggtcgtc ggccaggagg cgaagcgtag
     2461 gcatgatcca cggcgtacaa tcagcaccga cccaggtcaa accactggtt ggcacccgtt
     2521 ttgctgcgac cccctcagcc cacaggagac ttgacatgca gcgacgaagc ttcctcgccg
     2581 gaacggccgc ggcgaccgcc gtctcacgca aggtgatggc gcagaccacg aagacgcgga
     2641 tactccgcta cgtgccgcag gcggacctga cggtcatcga tcccgtaatg acgaccgcct
     2701 atatcacccg ctaccacgcg accatggtct gggatcaact ctatgggatc gacagcaagc
     2761 tgcagccgca gccgcagatg gtcgcaggcc atacagtcga ggacgacggc cgcctgtgga
     2821 cctttaccct gcgcgacaac ctgcgcttcc acgatggcga accggtgcgg gggcgggatt
     2881 gcgttgcgtc catcaaacga tgggcagcac gcgatccgat ggggcaggcc ctgctcgcgc
     2941 gggtggcgga aatgggcgcg ccagacgatc gccgtttcac cattcgcctg acccggccgt
     3001 tcagcctggt gctgaattgc ctgtccaaga tcggtccgcc gacattggtc gtgatgccgg
     3061 agcggctggc caacaccgat cctttccagt cgatcaagga cgtcgtcggc agcggcccgt
     3121 accgcttcgt cgcctcggag cgggtcgtcg gcagcaaagt ggtctatgtc cgcaacgccg
     3181 actacgtgcc gcgtccggac ggaacgcccg actggctggc gggcccgaag atcgtcaact
     3241 tcgaccgggt cgagtggacc gtcatgcccg accccgccac cgccgcggcg gcactgaaga
     3301 acggcgaggt ggactggtgg gagaatccgc ccaacgacct gctgccgatg ctcaaccgca
     3361 gcgccgacgt gcgcaccgag gccaccggcc cgctcggcac gatcggcacc ggcgtgttca
     3421 accacctgta tccgccgttc gacaagccgg cggtccggcg ggtgctgctg caggccattt
     3481 cccaggctga tttcatgacc gccgcggctg gcagcgaccc cgagatgtgg cgcgacggca
     3541 tcggcatctt cacgccgggc acgccgatgg cgaacaccgc cggcctggac atcgtcacca
     3601 gcccgaagcc cagcctgccg aacttggcaa aggctctgcg cgaagccggc taccagaacg
     3661 agcggattat cctgatgtcg cccagcgatc aggccgtgct gacggcccag tgcgcggtcg
     3721 cggtggacct gatgcagaag ctggggatgc aggttgacct gcagatgatg gactggggaa
     3781 cgctggtgca gcggcgcgcc agcaaggagc ctccggccaa gggcggctgg aacatgttca
     3841 tcaccggctg gtccgggctg gacatgacga cgccgatcac caaccagacc ttgcgctcca
     3901 acggcgccaa agcctggttc ggctggccgg atctgccaca ggtgcagtcg ctgatcgatg
     3961 cctgggtgga ggcgccggat accgcggcgc agcagaagat cgcagtcgaa atccaggcca
     4021 aggcaatgga acaggtgcct tacctgccaa ccggccagta tttctaccgc accgcgtgcc
     4081 gcaaggacat caccgggatc attccggggc agttcgtatt ctggaatgca cggaggacat
     4141 gacctgaccc gctaccgcat cggtaagccg gccatcgacg cgatatcccg tagaaagcca
     4201 taattgccgt ggtcgcgccc gcccccgctg aagtcccgcc tccgctgaaa tcccgcccag
     4261 cgctgaaatc ccgcccggcg ctgaaatggc gcttggcgct gaaatgccgc ccggcgctga
     4321 catgccgccc ggcgctgaaa tccggcatgg tggttaaatc ccgcatggat gttggatccc
     4381 ccatg
//
LOCUS       DASWQN010000960         3402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1146747, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000960 DASWQN010000000
VERSION     DASWQN010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146747"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VGC82_16410"
     CDS             complement(<1..435)
                     /locus_tag="VGC82_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0424902.1"
                     /translation="MPRRLEGDFDHIVVGAGTAGCIVANRLSADPKTRVLILEAGGSD
                     NWIWFHIPVGYLFAIGNPRSDWMFRTEPEAGLNGRSLAYPRGKVIGGSSAINAMISMR
                     GQAADYDHWRQLGNAGWGWDDVLPLFRKSEHRAGPADPLHGVG"
     gene            664..1836
                     /locus_tag="VGC82_16415"
     CDS             664..1836
                     /locus_tag="VGC82_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEY0424903.1"
                     /translation="MPIVNRVADLQPDIQAWRRDIHQHPELQYDVHRTAAFVADRLRE
                     FGCDEVATGLGRTGVVGVIKGRKPAGNGDIKVIGLRADMDALPIEEATSLPYASRTPG
                     KMHACGHDGHTAMLLGAARYLAETRNFAGDVVVIFQPAEEGGAGAAAMIEDGLFDRFA
                     IDQVYGMHNGPGIPVGSFAIRPGTIMAATDSIDLRIEGLGGHAARPHKCIDPVLAGAQ
                     LITALQGIVARNVDPLESAVISICEFHAGNARNVIPQTAELKGTVRTLTAEVRELVEK
                     RVREVVAGMAQVTGTKIDLEYTRGYPVVVNHGPQTDFATQVAREVAGDANVQEMAPLM
                     GAEDFAYMLEQRPGAFIFCGNGDSAGLHHPAYNFNDEAIVFGTSYWIKLVENSLAA"
     gene            complement(1852..3069)
                     /locus_tag="VGC82_16420"
     CDS             complement(1852..3069)
                     /locus_tag="VGC82_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424904.1"
                     /translation="MKPFSPIKTPPDILCLYGARALRGFGDGFAIIVLPVYLSAVGFS
                     PQQIGIVASASLLGTAVLTLVAGFIAPRYELRHLFFAGAGLIVFTGLVFPATETIAPV
                     LLVAFIGSINPSGGDLGMLVPLEHALLTKETPDRHRTGVFARYSLIGSLTAAVGSLAA
                     ALPEILAAHGWQRLGAIKLMFYGYAALGLLAALLYRRLPRDHTHAASVATPALGPSRN
                     IVYQLAALFSLDAFAGGFVVQSLLALWLFQRFNLSLEAASLYFFGSSLLGAMSFPVAA
                     WLARRIGLINTMVYTHIPSSLCLIAAAFSSSLTLVLSLLLVRSALSQMDVPTRSSYVM
                     AVVTPAERTAAASVTAVPRSLASSISPAIAGFMLAGPFSGLPLVVCGCLKIGYDLALL
                     RMFRHTRPPEEVR"
BASE COUNT          679 a         1087 c         1120 g          516 t
ORIGIN      
        1 ccccacgcca tgcagcgggt cggccgggcc ggcgcgatgc tcgctcttgc ggaacagcgg
       61 cagcacgtcg tcccagcccc agccggcatt gccgagctgg cgccaatggt cgtagtcggc
      121 ggcctggccg cgcatcgaga tcatggcatt gatcgcggac gagccgccga tcaccttgcc
      181 gcgcggatag gccagcgagc ggccgttgag gccggcttcc ggctcggtcc ggaacatcca
      241 gtccgaacgc ggattgccga tcgcgaagag gtagccgacc gggatgtgaa accagatcca
      301 gttgtcgctg ccgcccgcct ccaggatcag gacgcgggtc ttgggatcgg ccgacaggcg
      361 gttggcgacg atgcagccgg cggtgcccgc gccgacgacg atgtggtcga aatcgccttc
      421 cagccttctc ggcatttcag aattccgcgc cgtcaaaccg ttcctcccgt aatgcccggc
      481 gttcgtaatg cccggcgttc ggccggcatc cacgccttct ggccggcgac ggtgcgaaag
      541 cgtggatgca gcgcaggaag cccggccatg acggcttttg tcaatcaccc ggagttctgt
      601 tgggcggacc gcggcccgcg tgctacacag ggcaaaacgt caaccgttaa cccgagagtt
      661 gccatgccca tcgtcaaccg cgtcgccgat ctgcaacccg atattcaggc ttggcgccgg
      721 gatatccacc agcatccgga attgcagtac gacgtgcatc ggacggcggc gttcgttgcg
      781 gaccggttgc gggaattcgg ctgcgacgag gtggccaccg gactcggccg caccggcgtc
      841 gtcggcgtca ttaagggccg caagccggcc ggcaacggcg atatcaaggt gatcgggctg
      901 cgcgccgaca tggacgccct gccgatcgag gaggcgacca gcctgcctta cgcctccagg
      961 acgccgggca agatgcacgc ctgtggccat gacggccata ccgcaatgct gctgggcgcc
     1021 gcccgctatc tggcggaaac acgcaatttc gccggcgatg tcgtcgtcat cttccagccc
     1081 gcggaggagg gcggcgccgg cgccgcagcc atgatcgagg acgggttgtt cgaccgtttc
     1141 gccatcgatc aggtgtacgg catgcacaat ggcccgggaa ttccggtcgg atcgttcgcg
     1201 atccggcccg gcacaatcat ggccgccacg gattccatcg acctgcggat cgaagggctc
     1261 ggcggccatg cggctcgccc gcacaaatgc atcgatccgg tcctggccgg cgcccagctg
     1321 atcacggcgt tgcagggcat cgtggcgcgc aatgtcgacc cgctggaatc ggctgtcatc
     1381 tcgatatgcg aattccacgc cggcaacgcg cgcaacgtga tcccgcaaac cgccgagctg
     1441 aaaggtaccg tccgcaccct gaccgccgaa gttcgggagc tggtcgagaa gcgggtgcgc
     1501 gaggtcgtcg ccggcatggc gcaggtcacc ggcaccaaga tcgacctgga atacacccgc
     1561 ggttaccccg tggtggtcaa ccatggcccg cagaccgatt tcgcgaccca ggtggccagg
     1621 gaagtggcag gtgatgccaa cgtccaggag atggcgccgc tgatgggcgc cgaggatttc
     1681 gcctacatgc tggaacagcg gcccggcgcc ttcatttttt gcggcaacgg cgacagcgcc
     1741 gggctgcatc acccggccta taatttcaac gacgaggcga tcgtgttcgg cacgtcgtac
     1801 tggatcaagc tggtcgagaa ttcgctggcg gcgtgagcgc gacggccgcc gctatcgcac
     1861 ctcttccggc ggtctggtgt gccggaacat ccggagcagc gccaggtcat agccgatctt
     1921 caggcatcca cataccacca gcggcaggcc ggaaaacggg ccggccagca tgaacccggc
     1981 gatggcggga ctgatcgacg acgcgaggct gcggggcacg gcggtcacgc tcgcagccgc
     2041 cgtgcgttcg gccggcgtta ccacggccat cacataggac gaccgggtcg gaacgtccat
     2101 ctgcgacaga gccgagcgaa cgagcaacag cgacagcacc agggtcaggc tcgatgaaaa
     2161 ggccgccgcg atcaggcaca ggctggaggg gatgtgcgtg tacaccatgg tgttgatgag
     2221 gccgatgcgc ctcgcgagcc aggcggcaac cggaaatgac atcgcgccca gcaggcttga
     2281 gccgaagaaa tacaggctcg ccgcctcgag cgagagattg aatcgctgga acagccacag
     2341 cgccaacaac gactgcacga cgaagccgcc cgcgaaggcg tcgaggctga acaaggccgc
     2401 gagctggtag acgatattgc gggatggccc gagcgcgggc gttgcgacgc ttgccgcatg
     2461 agtgtggtcg cgcggcagcc gccgatagag caacgccgcc agcagcccga gggcggcata
     2521 gccgtaaaac atcagcttga tcgcgccgag ccgctgccag ccgtgggccg cgaggatttc
     2581 cggcaacgca gccgcaagcg atccgacggc ggcggttagc gacccgatca ggctgtaccg
     2641 tgcaaaaacg ccggtgcggt gccggtcggg ggtctccttc gttaacagcg catgctcgag
     2701 gggaaccagc atgccgagat cgccgcccga tggattgatg ctcccgatga aggccaccag
     2761 cagaacaggc gcaatagtct ccgtggccgg aaagaccagg cccgtgaaaa cgatcaagcc
     2821 ggcgccggcg aaaaacagat gccgcagctc gtaacgcggc gcaatgaagc ccgcgaccag
     2881 agtcagcacc gccgttccca gcagggatgc gctggcaacg attccgatct gctgcggcga
     2941 gaaaccaacg gccgaaaggt agaccgggag aacgatgatc gcgaagccgt cgccaaagcc
     3001 gcgaagcgcc cgcgcgccgt acagacaaag gatatcgggc ggcgttttga tcggggagaa
     3061 tggcttcatc ggtatatccg ccacttggtc gggcggacca aaaagctgcc ccagaacatg
     3121 tctcacctcc gcaacggagc gagacaactc ttggctggcg acagctgacc gggcggttgc
     3181 cgatggcccg aactccacgc ccgtaacacg gcagcccggt gcggacaatg gttttgcagt
     3241 tccggaacat agctcccgag gagacgttgg gacgccgggg acatcccaaa tcccagtgac
     3301 gtcttacgat ggagtttgcc atgaccgcaa aagacaagaa gctcaacgac ctgttccttg
     3361 atatgctgaa ggatatctat ttcgccgaaa agcagatctt ga
//
LOCUS       DASWQN010000961         7064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1280052, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000961 DASWQN010000000
VERSION     DASWQN010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280052"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VGC82_16425"
     CDS             complement(<1..318)
                     /locus_tag="VGC82_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424905.1"
                     /translation="MVVDSGFDSARPGPGRPDAAGDGKAGFGDARPGAFADIPDSQRL
                     AQDWITLWQSELSAMAADPEMRESWQTLTALWAGAVSAMMRGMPRPQAADGRDQPSGQ
                     TTTA"
     gene            469..1062
                     /gene="phaR"
                     /locus_tag="VGC82_16430"
     CDS             469..1062
                     /gene="phaR"
                     /locus_tag="VGC82_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY0424906.1"
                     /translation="MAVPVETLPDAKVGEKPPVVVKKYANRRLYNTESSSYITLDNLA
                     DMVRLGRDFVVYDAKSGDDITRSVLTQIIVEEEGKGQNLLPTAFLRQLIGLYGGSMQG
                     LVPKYLEHAMTSFAQQQEQMRTAMQKTMGTIFPFGNIEEVGRQNMAMMERAFSMFTPF
                     YRGTEPTNPSDVHSSEIEALRSEIERLRKELEIAKKR"
     gene            complement(1107..1736)
                     /locus_tag="VGC82_16435"
     CDS             complement(1107..1736)
                     /locus_tag="VGC82_16435"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY0424907.1"
                     /translation="MTKVKICGINDPAAFDTAVEAGADWVGFVFFPPSPRYVTPEIAA
                     GLSARLRGGPPRVGLFVEPTLAAIAAVLDIVKLDILQIYGALDDLPTIRARFGLPVWR
                     AAGIDSAADLPTSLLGADRLLLEAKPPAGADRPGGNATTFDWSMLRGWAAPAPWILAG
                     GLTPDNVATAIRETGSGAVDVSSGVEVRKGLKDAALIRSFLAAAKRTGR"
     gene            complement(1733..2425)
                     /gene="pyrF"
                     /locus_tag="VGC82_16440"
     CDS             complement(1733..2425)
                     /gene="pyrF"
                     /locus_tag="VGC82_16440"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY0424908.1"
                     /translation="MPGGGSLIVALDTSDANQAAVWAMAVAPHVGLFKIGLEFFLANG
                     ITGFRRIAGAPIFLDLKLHDIPNTVAGGVRAVLPLQPKMLTVHGSGGSAMIRAAHQAA
                     AVVGQDRPMILAVTVLTSLDQADLRAVGVDATPADQVLRLGRLAIEAGADGLVCSPLE
                     VAMLRQALGPAVKLVVPGIRPKGSAASDQVRTMTPAQAIAAGADWIVVGRPITAAADP
                     AAAAAHIADSIR"
     gene            complement(2425..2730)
                     /locus_tag="VGC82_16445"
     CDS             complement(2425..2730)
                     /locus_tag="VGC82_16445"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEY0424909.1"
                     /translation="MLFLIVSFILCIPLVLFALSNTEIVRLGFWPTDYGVDVHVSLAI
                     LVAMAVAFLLGALVVWISELGQRRRARQAERTVRLLEAQIEELKARVGPTMSLPQGS"
     gene            complement(2747..3061)
                     /gene="ihfB"
                     /locus_tag="VGC82_16450"
     CDS             complement(2747..3061)
                     /gene="ihfB"
                     /locus_tag="VGC82_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEY0424910.1"
                     /translation="MTKSELIAELATANPHLRGQDVETIVATIFEEISFALARGERVE
                     LRGFGAFTVKHRDARTGRNPRTGASVSVDEKSVPFFKAGKELRVRVNAGKTPRAALRR
                     QA"
     gene            complement(3077..4000)
                     /gene="sppA"
                     /locus_tag="VGC82_16455"
     CDS             complement(3077..4000)
                     /gene="sppA"
                     /locus_tag="VGC82_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY0424911.1"
                     /translation="MSLETDLLLDRRRLKRRLVFWRVFSVLALVVAGLAGLRGAGLTS
                     LGAHITRVSVTGLITEDRKTTEAIDSLADNNRVKALIVSIDSPGGSVAGGETLHDAIA
                     RVAAKKPVVVTMGGLAASAGYMIALPANRIFARDATLTGSIGVLLETGELSGLLGKIG
                     ISAEVVRSGPLKDEPSLVRPLSAQGKEVLQGLVNDMYNQFVEMVAAGRHMDVAKVRSL
                     ADGRAYTGRQALQLGLVDAIGGEREAKDWLVSSKGLDPGLPVEDLPTTGFASRALAGQ
                     IGPMLQEIWKTLISQSLSLDGAWAVWHRSGS"
     gene            complement(4022..5758)
                     /gene="rpsA"
                     /locus_tag="VGC82_16460"
     CDS             complement(4022..5758)
                     /gene="rpsA"
                     /locus_tag="VGC82_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY0424912.1"
                     /translation="MALSAVARAHEPAPDHFRGEDFASLLDETLGTDTSFDGSVVTGR
                     VIRLTDEFAVVDVGLKSEGRVALKEFGPPGAKPDVKPGDMIELYVERYEDRDGAIILS
                     REKARREEAWTNLEKAFEAQKRVDGTIYGRVKGGFTVDLGGAVAFLPGSQVDIRPVRD
                     VGPLMGTPQPFQILKMDRARGNIVVSRRAVLEETRAEQRSELIQGLKEGMILDGVVKN
                     ITDYGAFVDLGGVDGLLHVTDIAWRRINHPSEALTIGQQVKVQVIRFNSETQRISLGM
                     KQLEADPWEGVTAKYPPGAKYSGRVTNITDYGAFVELEPGVEGLVHVSEMSWTKKNVH
                     PGKIVSTSQEVDVMVLDVDSSKRRISLGLKQVQRNPWEQFAEEHPIGSEVEGEIRNIT
                     EFGLFIGLSADIDGMIHMSDLSWDDAGETAMAGYEKGQVVKAKVLDVDVEKERISLGV
                     KQMRDDPAATILDTVHKGDVVTCIVSAVQANGIEVKVNDVLTGFIRRAELARDKGDQR
                     PDRFAVGEKVDAKITAVDRQSRKLTLTIKGKEVEEEKVAMAEFGSSDSGASLGDILGA
                     AIRRRNQLAQDS"
     gene            complement(6074..>7064)
                     /locus_tag="VGC82_16465"
     CDS             complement(6074..>7064)
                     /locus_tag="VGC82_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0424913.1"
                     /translation="LGVEVPFLTDKHEVGRLAHALEVFKINRRKADELGAEQQAEQMA
                     KRRRQETIERVIGEFQDRTTRVADTVAHAARSVQSHAESLAAMAQQSRTQIGLVNQAA
                     VDTNGNVHTIASAAEELSAAVSEVTQQVTRSTAVAERSVAEADRTSETIRGLAEASQR
                     IGAIVQVIQDIASQTNLLALNATIEAARAGEAGKGFAVVASEVKTLANQTTKATAEIQ
                     QQINGIQTETGRVVEAIASISKTVVDMRVIATGIAAAMEQQGMTTAEIARNINLAAGS
                     TQEVSSNISGAADAAETTKRSVGELHTASDALLREAMLLSEGMTNFFQQMRAA"
BASE COUNT         1271 a         2312 c         2167 g         1314 t
ORIGIN      
        1 agcggtcgtg gtctgtccgc tcggttggtc acgtccgtcg gcagcctgcg ggcgtggcat
       61 cccgcgcatc atcgccgaaa ctgctccggc ccatagggcc gtcaaggtct gccaggattc
      121 gcgcatttcc ggatcggccg ccattgccga cagttcgctc tgccacagcg tgatccaatc
      181 ctgcgccaat cgttgcgaat ctggtatatc cgcgaaggcg ccgggtctgg catcgccgaa
      241 gccggccttc ccgtcgcccg ccgcatccgg ccgacccggg ccgggacggg cactgtcgaa
      301 acccgaatcc accaccatgc cgcattcatc ccccttgcag gccgagtcac aggcgttgcc
      361 ggagatcggc caaacgtcaa accgggcacc agcgggataa ggaacgggaa gggctctatc
      421 gcaagtgcga aatacgcggt agggagggga ccctgatacg aggtatgcat ggctgtgccc
      481 gtcgagaccc tgccggatgc gaaggtcggt gaaaaacctc cggtcgtggt aaagaaatac
      541 gcgaaccggc ggctttataa caccgaaagc tctagctaca tcacgttgga taacttagcc
      601 gacatggtga ggctgggacg tgatttcgtc gtctatgatg ccaaaagcgg cgacgacatc
      661 actcgaagcg tgctgaccca gatcattgtc gaggaagaag gcaaagggca gaacctgctg
      721 cctaccgcct tcctgcggca actaatcggg ctgtacggcg ggtcgatgca ggggttggtg
      781 ccgaaatacc tcgagcacgc catgacgtcg ttcgcccagc aacaggagca gatgcgcacc
      841 gcgatgcaga agacgatggg gacgattttc cccttcggca acatcgagga ggtcggccgg
      901 cagaacatgg ccatgatgga acgcgcgttc agcatgttca cgccattcta ccggggcacc
      961 gaaccgacca acccgtccga cgtgcactcg tcggagatcg aggccctgcg gtcggagatc
     1021 gagcggctgc gcaaggaact ggaaatcgcg aaaaagcgat aaatctccgg tggcctgcta
     1081 ccggggcacc gccgtcgtcg cgtgcctcaa cgtccagtgc gcttggccgc cgccaaaaat
     1141 gaccggatca gcgcggcgtc cttgagtcct ttgcggactt ccacgccgga cgagacatcg
     1201 accgcgccgc taccggtttc gcggatcgcc gtcgcgacat tgtccggcgt caacccgccg
     1261 gccaggatcc agggcgcggg ggcagcccaa ccgcgcagca tcgaccaatc gaatgtcgtc
     1321 gcgtttccac ccggccggtc cgctccggcg ggcggtttgg cttccagcag caaccggtcg
     1381 gcaccgagca gggaagtcgg aagatcggcg gcactgtcga tgcccgccgc ccgccaaacc
     1441 ggcagcccaa agcgcgccct gatggtgggc agatcatcca gtgctccgta gatctgcaag
     1501 atatccagtt tcacgatgtc gagcacggcg gcgatcgccg ccagcgtcgg ctccacgaac
     1561 agaccgaccc gcggcggccc gccacgcaat cgcgcggaca gcccggcagc gatttcgggt
     1621 gtcacgtagc gcggggaggg cgggaagaac acgaagccga cccagtcggc cccggcctct
     1681 accgcggtgt cgaacgcggc cgggtcattg atgccgcaaa tcttgacctt ggtcatcgaa
     1741 tgctgtccgc gatgtgcgcc gccgccgccg ccgggtcagc ggcagcggtg atcggacgcc
     1801 cgacgacgat ccagtcggcg cctgcggcga tggcctgcgc cggggtcatc gtccgaacct
     1861 gatcgcttgc cgccgatccc ttcgggcgga tacccggcac caccagcttg accgccggac
     1921 ccaacgcctg tcgcagcatc gcgacttcca gtgggctgca tacaagcccg tccgcgcccg
     1981 cctcgatcgc cagccgtcca aggcgcagga cttgatcagc gggcgtggcg tccacaccca
     2041 cggcgcgcag atcggcctgg tcgagactgg tcaggacggt caccgcgagg atcatcggcc
     2101 gatcctggcc aacgaccgct gctgcctgat gggccgccct gatcatcgcc gacccaccgg
     2161 aaccatggac ggtcaacatc ttcggttgaa gcggcagcac cgcccgcacg ccacccgcca
     2221 cggtgttcgg gatatcgtgc agcttcaggt ctaggaaaat cggcgcgccg gcgatgcgtc
     2281 ggaaacctgt aatgccgttc gccaggaaga attccaggcc gatcttgaac agcccgacat
     2341 gcggcgccac ggccatcgcc caaaccgcgg cctggttcgc atctgaagtg tccagcgcga
     2401 caatcaggct gccgccgccc ggcatcagct accttgcggc agcgacatcg tggggccaac
     2461 gcgggccttc aattcttcga tctgcgcttc aagcaggcgc accgtccgtt cggcctgacg
     2521 cgcccggcgg cgttgtccaa gttcggatat ccagaccacg agcgccccga gcaggaacgc
     2581 cacggccatg gcgaccagaa tggcgagcga gacatgcacg tcgacgccgt agtccgtcgg
     2641 ccaaaagccc aacctgacga tttcggtgtt cgacagcgcg aacaacacca gcggtatgca
     2701 caggatgaag ctgacgatca ggaacagcat gggacggcgg aacctttcag gcttgccggc
     2761 gcaaagctgc ccgaggggtt tttccggcat tcacccgcac gcgcagttct ttcccggctt
     2821 tgaaaaaggg gacagacttc tcgtcaaccg aaaccgaggc acctgttctt gggttgcgtc
     2881 cggtgcgggc atcgcggtgc ttgaccgtga acgcgccgaa gccacgcagt tcgacccgtt
     2941 cgccgcgggc cagcgcgaag ctgatttcct cgaaaatcgt cgcgacgatc gtttccacgt
     3001 cctgcccacg gaggtgcgga ttggcggtcg ccaactctgc gataagttca gatttggtca
     3061 aggcattccc ctggaatcag ctcccggaac gatgccagac agcccaggct ccgtcaagac
     3121 taaggctttg tgaaatcagc gttttccaaa tttcctggag cattggtcca atttggccgg
     3181 ccagtgcgcg actggcgaag ccggtggtgg gaagatcctc gaccggaagg ccggggtcca
     3241 ggccctttga cgagaccagc caatccttgg cttctcgttc gcctccgatc gcatccacca
     3301 agcccagctg cagcgcctgg cgccccgtgt aggctcggcc gtccgcaagc gagcggacct
     3361 tcgccacgtc catatgacgt cccgcggcga ccatttcgac gaactgatta tacatgtcgt
     3421 tgacgaggcc ctgcagaacc tccttgcctt gcgccgacaa aggccgcacc aggctgggtt
     3481 cgtctttcag cggcccggaa cgcaccacct cggcactgat gccgatcttg ccgagcaggc
     3541 ccgacaattc gccggtttcc aacagcacgc cgatcgagcc ggtcagcgtc gcgtcacggg
     3601 cgaaaatacg gttcgccggc aaggcaatca tgtaaccggc ggacgccgcg agtccgccca
     3661 tggtgacgac gaccggcttt ttcgcggcca cccgggcgat cgcgtcgtgc agggtttcgc
     3721 cgccggcgac actgccgccg gggctgtcga tcgacacgat gagtgccttt acccgattgt
     3781 tgtcagccag cgaatcgatg gcctcggtcg tcttgcggtc ttcggtgatc aaaccggtga
     3841 ccgaaacccg agtgatatgc gcacccaacg acgtcagtcc ggcaccgcgc agtccggcca
     3901 acccggccac aaccaacgcg aggacggaaa atacccgcca aaagacaagg cggcgcttga
     3961 ggcgccgcct gtccaaaagc agatcagtct ccagcgacac tgccggtatc cggccgggtc
     4021 gtcagctatc ctgggcgagc tggttgcggc gacggatcgc agctcccagg atatcgccaa
     4081 gcgatgcgcc ggagtcggac gatccgaatt ccgccatggc aactttctct tcctcgactt
     4141 ctttaccctt gatcgtcagg gtcagcttcc gggattggcg atcgaccgcg gtgatcttcg
     4201 catcgacttt ttcgcccacg gcaaagcggt cggggcgctg gtcgccttta tccctggcca
     4261 gttcggcgcg gcggatgaag ccggtcagaa cgtcgttgac cttgacttcg atgccgttgg
     4321 cctgaaccgc gctgacgatg caggtcacga cgtcgccctt gtggacggta tcgaggatgg
     4381 tcgcggcagg atcgtcacgc atctgcttga cacccaggct gatgcgttcc ttctcgacat
     4441 cgacgtcgag taccttggcc ttgaccacct ggcccttttc atagcctgcc atcgcggttt
     4501 cgccggcgtc gtcccatgac aggtcggaca tgtggatcat gccgtcgatg tccgcggaca
     4561 agccgatgaa caggccgaat tccgtgatgt tgcggatttc gccctcgacc tcggagccga
     4621 tcggatgctc ttcggcgaac tgctcccacg ggttgcgctg cacctgcttg aggcccagcg
     4681 aaatacgccg cttggagctg tccacgtcca gcaccatgac gtcgacttcc tggctggtgg
     4741 acacgatctt gccgggatgg acgttctttt tcgtccacga catttccgat acgtgcacca
     4801 ggccctcgac gccgggctcc agctccacga aggcgccgta gtcggtgatg ttggtgacgc
     4861 ggccggagta tttcgcgcct ggcgggtact ttgcagtgac gccttcccac ggatcggctt
     4921 ccagctgctt catgcccagg ctgatgcgct gggtctcgga gttgaagcgg atgacctgaa
     4981 ccttgacctg ctgaccaatc gtcaaggcct cggacggatg gttaatgcgg cgccatgcga
     5041 tgtcggtgac atgcagcagc ccatcgacac cgccgaggtc cacgaacgcg ccgtaatcag
     5101 tgatgttctt gaccacgccg tccaggatca tgccttcctt gaggccctgg atcagctcgc
     5161 tgcgctgctc cgcacgcgtc tcttccagaa ccgctcggcg tgacacgaca atgttaccgc
     5221 gcgcacgatc catcttgagg atctggaacg gctgcggcgt ccccatcagc ggcccgacat
     5281 cgcgcactgg acggatatcg acctggctac cgggcaggaa tgccaccgcg ccgccgaggt
     5341 caaccgtgaa gccgcccttg acccggccgt agatcgtgcc gtcaacgcgc ttctgcgcct
     5401 cgaatgcctt ttccaggttg gtccaggcct cttcgcgacg agctttctca cgcgacaata
     5461 tgatcgctcc atcgcgatct tcgtaacgct cgacatacaa ctcgatcatg tcgccaggct
     5521 tgacgtcggg cttggcaccg ggcgggccaa actccttgag ggcgacgcgg ccctcggatt
     5581 tcaggccgac gtccacgacg gcgaactcgt cggtcaagcg tatgacgcgt cccgtgacga
     5641 cggagccgtc gaaactagtg tccgtgccga gcgtttcatc taggagggag gcgaaatctt
     5701 cgccccggaa atggtctggc gcgggctcgt gagcgcgcgc gacggcggat aatgccatga
     5761 ggtcggtttg ttccgttacg gatgttcggg cgatcgcccg aattctgccg tggtatgcgc
     5821 gctccgtctt ttgaaacacg acttgcccgc gtccaaaggc gcaggccgga ttgaattgat
     5881 ggagtgcctg taggcttttg tttccaccac ggtgaaatag agatttcgtg agcggtgtca
     5941 agcctgttga atcgtttcaa cccctgttga aaccagcccg gcgctgcgga cagcacttag
     6001 ggcgcgttcg aacgcataaa cggcggctgg aatggccggc atcgcaccgg ccataacgac
     6061 tgaaatggcc ggatcaggct gcccgcattt gctggaagaa attcgtcatt ccctcgctga
     6121 gcagcatcgc ttcgcgaagc agcgcatcgg aagccgtatg cagttcgcca actgaacgct
     6181 tggtcgtttc cgccgcatcc gcggcaccgg agatgttgga tgagacttcc tgggtgctgc
     6241 ctgccgccag gttgatgttg cgagcgatct ccgccgtggt catgccctgc tgttccatcg
     6301 ccgcggcaat acccgttgcg atgacccgca tgtccacgac ggtcttgctg atgctcgcga
     6361 tcgcttcgac gacacggccg gtctcggtct ggatgccgtt gatctgctgc tgaatttcag
     6421 ccgttgcctt ggtcgtttgg ttggcgagcg tcttgacctc gctcgcgacc acggcgaagc
     6481 ccttgccagc ctcgccggcc ctcgctgcct cgatcgtggc attgagcgca agcagattgg
     6541 tctggctggc gatgtcctgg atcacctgca cgatggcgcc gatcctctgg gatgcctcgg
     6601 caaggccgcg gatcgtctcg ctggtgcggt cagcttcggc tacggaccgt tccgcgacgg
     6661 cggtggagcg cgtaacctgc tgcgtgacct cgctgaccgc ggccgaaagc tcctccgcgg
     6721 cgctggcgat cgtgtggacg ttgccattgg tatcgaccgc ggcctggttc accaagccga
     6781 tctgggtgcg gctctgctgc gccatggcgg ccaggctctc ggcatgcgac tgcacactgc
     6841 gcgccgcatg ggcgacggtg tcggcaaccc gcgtggtgcg gtcttgaaac tccccgatca
     6901 ccctctcgat cgtttcctgc cgccgtcgct tcgccatctg ctcggcctgc tgctccgcgc
     6961 caagttcgtc cgccttgcgc cggttgatct tgaacacttc cagcgcgtgc gcgagccgcc
     7021 cgacttcgtg cttgtcggtc aggaacggaa cctcgacgcc cagg
//
LOCUS       DASWQN010000962         3977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1015649, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000962 DASWQN010000000
VERSION     DASWQN010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015649"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1825)
                     /gene="cydC"
                     /locus_tag="VGC82_16470"
     CDS             complement(194..1825)
                     /gene="cydC"
                     /locus_tag="VGC82_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEY0424914.1"
                     /translation="MTGPLFRIMGLWGRQTGWLLAGLMAALAALIAGVSLMAMSGVTI
                     GAALLTASLAVPATLRGIGGARVVLRYVERLVTHGAMFRALADMRVWFFGRLARTSAG
                     GLGFRQAGDALARLVGDVEALDGLYLRILLPLAGAVLLLPVLALLVGHYLPGLATEVG
                     VLFAAAAFALPWLAAKASGQAGAGLAAEAGALRIAALDALTGLREVRAFAAEGRMLAA
                     VQAKEAALLSAQQGMASRTALAGAASFLLAQIALLAVLIEAGARPAGAIAAAFLVLAA
                     FEAVSGLSRAGALAGHASAAAARVLEAADAPVPVPDPADPVAMPVGTSLTFEAVEFRW
                     QPDRPLVFNGLTLEIPQGARVALLGPSGAGKSTLAALALKVVAPRSGRIRLDGVDIAT
                     LPAALVRARIGWLGQTTHLFDDTIQANLRLARPDADEAALWTALDDARIGDVVRGLPD
                     GLATWVGEGGARFSGGQGRRLALARALLSPAPILILDEPGAGLDAETEREFLETLNGT
                     AAGRTVILITHRLTGVEKLDRIFRLSGGKAVAAAG"
     gene            complement(1822..3510)
                     /gene="cydD"
                     /locus_tag="VGC82_16475"
     CDS             complement(1822..3510)
                     /gene="cydD"
                     /locus_tag="VGC82_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEY0424915.1"
                     /translation="MTQADSTKTAARSWLRREQQAGSKAARPVLMWHLLGMAAGIGQA
                     FAAASVLAGAFTGSEPEASALAIFGVLAVLRAGIAFLTERAAFAAGAAARRRLRSDAL
                     SRLLHSGPALLRTRHSGDLASIVVDKIEALDGLFSRYVPAAAFAIAGPAVVLLAVLYA
                     DPWAALVLLGCGLLVPLGMAVAGIGAAAASRDQFLAMARLQARFLDRVRGIATIVLYG
                     RAEDEARSLAQAAAELRVRTMRVLRVAFLSSAVLDLAAALAMVVLAIHYFGVLRTDGH
                     SQVLWQALAVLLLTPEFFAPLRVFSAAYQDRLHATGAAESLIDLPPLPEPAEAREIRT
                     VAAQGVTVAFDNVRLTWDASRGPALDGLSFRVSAGETLVLAGPSGAGKSSVIEILLGF
                     VRPDGGRVTINGADITDLVPQALSRLTAWIGQRPILFAGSIRDNILFARPEASVDEVT
                     KAATAARLDSFTAGLPMGLDTIVGEGGYGISGGQAQRVAIARAYLKNAPLLLLDEPTA
                     HLDPATEQEVLDSLRRLALGRTVILASHSSAAHGFAGRKLDLRDGKVIAARGAA"
BASE COUNT          746 a         1434 c         1265 g          532 t
ORIGIN      
        1 acgtcgccga gagcgccacc gggctgcggg ttcatgacat tcaccgaggt cgctagcgcg
       61 gtttcaccgt gactggaaca gcgttcgctc gcggctgcaa aggaagtcgt gggtggccgc
      121 gccaagcgca gtcatgacac acaggcgtca ggcggcgaac tggccgacac cttttccgcc
      181 cagtatgatc acgctaaccg gccgctgcga ccgccttgcc acccgacagc cggaaaatcc
      241 ggtcgagctt ctcgacaccg gtcaggcggt gggtaatcag tataaccgtc cgtccggccg
      301 cggtcccatt cagcgtttcc agaaattccc gttcggtctc ggcatccagc ccggcacctg
      361 gctcgtccag gatcaggatc ggcgcgggtg acagcagtgc ccgtgccagg gccaggcgac
      421 gcccttggcc gccggagaac ctggctccgc cctcaccgac ccaggtcgcc agtccatcgg
      481 gcagcccccg caccacatcg ccgatccggg catcgtccag tgccgtccat aacgccgcct
      541 catccgcgtc cggccgcgcc agtctcaggt tggcttggat ggtgtcgtcg aacaggtggg
      601 tcgtctgccc cagccaaccg atccgcgcac gcaccaacgc ggctggcagc gtggcaatat
      661 cgacgccatc gagccggatc cggcccgaac gaggggccac caccttcaat gccagcgccg
      721 ccagggtcga ttttcccgcc ccggacgggc caagcaatgc gacccgcgca ccctgcggta
      781 tctcgagcgt caggccattg aaaaccaggg gccggtcggg ctgccagcgg aactccaccg
      841 cctcgaacgt cagtgatgtg cctactggca tggcgacggg atcggcaggg tcgggcaccg
      901 gcaccggggc atccgccgcc tccaggaccc gcgccgctgc cgccgaggca tgcccggcca
      961 gcgcaccggc acgcgacagg ccgcttacgg cctcgaacgc cgccagcacc agaaaggccg
     1021 ccgcgatggc cccggccgga cgggcgccag cctcgatcag aaccgctagc agggcgatct
     1081 gcgccaacag gaaggatgcg gccccagcca gggccgtccg gctggccatg ccctgttggg
     1141 ctgacagcaa ggcagcctct ttcgcttgca cggcggccaa catgcgcccc tcggccgcga
     1201 aggcccgaac ctcgcgcagg ccagtcaagg catccagcgc cgcgatacgc agcgctcccg
     1261 cctcggcggc caatccggcg cccgcttgcc ccgaagcctt ggccgccagc catggcaacg
     1321 caaaggccgc ggcggcgaac agcacgccga cttcggtggc caagccagga aggtaatggc
     1381 ccaccagcaa cgccagcacc ggcagcaaca gaacggcgcc ggccagcggc agcaagatgc
     1441 gcagatacag gccgtcgagc gcttccacat cgcccaccaa tcgcgccagc gcatcccctg
     1501 cctggcggaa gcccaagccg ccggccgagg tgcgagccag gcgtccgaag aaccagaccc
     1561 gcatgtccgc cagggcgcgg aacatcgcgc cgtgcgtcac caaccgctcg acatagcgca
     1621 gcaccacccg ggcaccgccg atcccgcgca gggtcgcggg gaccgccagc gaggcggtca
     1681 gcagcgccgc accgatggtc acgccggaca tcgccatcag gctgacgccc gctatcagcg
     1741 cggccagcgc cgccatcaac ccggccagca gccaaccggt ctgccggccc cacaatccca
     1801 tgatccggaa caacggcccg gtcatgcggc acctcgcgcc gcgattacct tgccgtctcg
     1861 cagatccagc ttacgcccgg caaagccatg cgcggccgag gagtgactgg ccaggatcac
     1921 cgttcggccc agagccaggc ggcggagact gtccaacact tcctgttcgg tagccggatc
     1981 caaatgtgcg gtgggctcgt ccagcagcag cagtggcgcg ttcttcaagt aggcgcgtgc
     2041 gatggcgacc cgctgcgcct ggccgccgga gatcccgtag ccgccttcgc cgacaatcgt
     2101 gtccagcccc atcggcaagc cggcggtgaa ggaatctagc cgcgcggcgg tggcggcctt
     2161 ggtcacttcg tcaacggagg cctccggccg agcgaacagg atgttgtcgc ggatggagcc
     2221 ggcaaacagg atcggacgtt ggccgatcca ggccgtcagc cgggacaaag cttgcggcac
     2281 caggtccgtg atgtccgcac cgttgatcgt cacccgtcca ccgtcggggc gcacgaaccc
     2341 cagcaggatt tcgatcaccg aagactttcc ggcgcccgaa ggcccggcca gcaccagcgt
     2401 ttcccccgcc gacacacgga acgacaaacc gtccaatgca ggcccgcggg aggcatccca
     2461 ggtcaggcgg acattgtcga atgcgacagt cacaccctgc gcggccacgg tgcggatctc
     2521 gcgtgcttcc gcgggctcag gcaaaggcgg caggtcgatc agcgactctg ctgcgccggt
     2581 ggcatgcagg cgatcctgat acgcggcgga aaacacccgt agtggcgcga aaaattctgg
     2641 agtcagcagc aacactgcga gcgcctgcca cagcacctgg ctgtggccat ccgtccgcag
     2701 aacaccgaaa taatggattg ccagaaccac catcgccaac gcggcggcga gatcaagcac
     2761 agcggacgac aggaacgcca cccgcaggac ccgcatggtg cggacgcgca gttccgccgc
     2821 cgcttgggcc agcgaccggg cttcatcctc ggcccgtcca tacagcacga tcgtcgcgat
     2881 gccgcgcacg cggtcgagga accgggcctg caagcgggcc atcgccagga actggtcccg
     2941 cgaggccgcg gccgcgccaa ttccggcgac cgccatgccg agcggcacca gcaggccgca
     3001 gcccaggagc accagcgcag cccatggatc ggcatacagc accgccagca gtacgacggc
     3061 gggaccggcg atcgcgaaag cggcggctgg aacatagcgc gagaacagac cgtccagcgc
     3121 ctcgatctta tccacgacga tgctcgccag gtcgccggaa tgacgcgtgc gcagcagtgc
     3181 cggccccgaa tgcagcaacc gggacagcgc atcgctgcgc aaccgccggc gcgcggcggc
     3241 cccggcggca aaggcggccc gttcggtcag gaaggctatc ccggcgcgca acaccgccag
     3301 cacgccgaaa atcgccagcg ccgaggcctc cggctccgat ccggtaaagg cacccgccag
     3361 caccgaggca gcagcgaagg cctgtccgat gcccgcggcc atccccagca gatgccacat
     3421 cagcaccggc cgagcggctt tgctgccggc ctgttgttct cggcgcagcc aggagcgtgc
     3481 tgctgtctta gtggaatccg cctgtgtcat gagttcgtga attaccctat ggccagaggg
     3541 gatgacagtg cgaaaatcta cgcagccgct gaacagggca catgccgccc cgaagagcag
     3601 ccgcggttga tgatcacgtc atggccgtgc ctgacgcggt cgcccacgac tcgtgttaac
     3661 agggaaagcc gtgggcgacg ggcacgatgc cggtcatgac acgagcggca aagccgcgat
     3721 ttggttggtc gacgcgtatg gtgtgatgcc ggcgacgaca ttgttgatgt gatgccggcc
     3781 acggcattgg taaaccattt tcagcctgac cgtgcaggcc gcaaactgcc tgtcccctca
     3841 tttgatcgcg tgattcacgc gcttcgcacg ttccgctccg cgtgatcacg ctccagaccg
     3901 gatcgcccgg tttggagcgg ctgttgtcat cacaccccaa ggttgggcaa cctcagggct
     3961 tcggcactca ggcggcg
//
LOCUS       DASWQN010000963         1519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_488038, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000963 DASWQN010000000
VERSION     DASWQN010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488038"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VGC82_16480"
     CDS             <1..738
                     /locus_tag="VGC82_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HEY0424916.1"
                     /translation="VRMQAAVQRHVDSAISKTVNVPENIGFEAFQAVYLDAYRSGCKG
                     CTTYRPNAITGSVLEATAAEPAAAIPPTTGDLLVRSEKLTGATYKLRWPDSEHAMYVT
                     LNDIEQDGIVRPFEIFVNSKNLEHYAWVVALTRMISAVFRRGGEVAFVAEELKQVFDP
                     RGGQWSNGRYVGSLVAAIGDIIERHMVETGFLAPAETLVAKAVGQPAAGASATPLGPL
                     CPKCSQPGLVREAGCLSCVHCGWSKCS"
     gene            1059..>1519
                     /locus_tag="VGC82_16485"
     CDS             1059..>1519
                     /locus_tag="VGC82_16485"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424917.1"
                     /translation="MGTEARVVAFVCASEILGLAGFSLVPALLPQFIATWSLSNTEAG
                     WLAGMMSGGYMLGVLPLVALTDRIPARTIFLICSALSALSSFGMALSDSLPPALVWRG
                     VGGIVLAGVYMPGLRALTDGMAGVRRARAAAWYTSSFTLGSSLSFLLGQVG"
BASE COUNT          264 a          470 c          490 g          295 t
ORIGIN      
        1 gtccgcatgc aggctgccgt gcagcgccat gtcgacagcg cgatctccaa gacggtgaat
       61 gtcccggaga acatcggctt cgaggcgttc caggcggttt acctcgacgc ctatcggagc
      121 gggtgcaagg gatgcactac ctaccggccg aatgcgatca ccgggtccgt gctggaggcc
      181 acggccgccg aacccgcggc ggcgatcccg ccaacgacag gcgatctgct tgtgcggtct
      241 gaaaaactca ccggtgcgac ctacaagctg cgctggcccg acagcgaaca cgcgatgtat
      301 gtaacactca acgacatcga gcaggatggc atcgtccggc cgttcgagat cttcgttaac
      361 tcgaagaacc ttgagcatta tgcctgggtc gtggctttga cgcgcatgat cagcgccgtg
      421 ttccggcgcg gcggcgaggt ggccttcgtg gcggaagagc tgaaacaggt attcgatccg
      481 cgcggcgggc agtggagcaa tggccgctat gtcggttcgc tggtcgccgc gatcggcgat
      541 atcatcgagc ggcacatggt cgaaaccggc tttctcgccc ccgctgagac gttggtggcg
      601 aaggcggtcg gtcagccggc ggcaggagcc tccgccacac cgctcggtcc actgtgtccc
      661 aaatgcagcc agccaggtct ggtgcgcgaa gcaggatgcc tgagctgcgt tcactgcggc
      721 tggtcaaaat gtagctgaag cttggagtga tagcaaattc cctggtccct gggatgacga
      781 cccgcctatc gccaagcccg gcaaccgtta cagcgtaacc gtttcgccat tgacggcggg
      841 atccaattcg cgctccactc gaaggccatg ccagtgatcc ttctgacatc gcaggaactc
      901 gatcagtgaa tggaagcgcc ggtcgaagag gccctgcaac tccagcgccc gctatcggat
      961 ggaagcctca agatcgtggc tcgcgaggcc agggaaggtg gcgaactgga gacgactgca
     1021 ggcactggct gacttctctg aaacaagcct agagtgtgat gggtactgaa gcgcgcgtcg
     1081 ttgcgttcgt ctgcgcctcg gaaatcctgg gcttggccgg cttctcgctg gtccccgccc
     1141 tgctaccgca gttcatcgcg acctggtcgc tgagtaacac cgaggctggc tggctcgccg
     1201 gcatgatgtc cggcgggtat atgctgggcg tgctgccgct tgtcgcgtta accgatcgca
     1261 taccggcgcg aacaatcttt ctcatctgca gcgcactgag cgcgctgtcc tccttcggca
     1321 tggctctgag cgatagcttg ccgccggcgc tggtctggcg aggcgtgggc ggcatagtcc
     1381 tcgccggcgt gtacatgccg ggcttgcgcg ccttgaccga tgggatggca ggggtcaggc
     1441 gtgcccgcgc cgccgcctgg tacaccagtt ctttcaccct cggctcgtcg ctttccttcc
     1501 tgctcggcca agtgggctt
//
LOCUS       DASWQN010000964         2761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1675990, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000964 DASWQN010000000
VERSION     DASWQN010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675990"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..2474
                     /locus_tag="VGC82_16490"
     CDS             435..2474
                     /locus_tag="VGC82_16490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEY0424918.1"
                     /translation="MACSLPLFVFGFGNAYFDYQADRAEAGRRALNMTRSLALLVQRD
                     LEARLSTLQVLAMSQALQAGDIASFRVRAAAVLRLQLPGANIVLLREDGQQVMNTALP
                     AGAALPARRDNTSLHLLFATAQPSVSDIFISVVDRPTLALDVPVRSPDGSVLYSLTLN
                     PTLAAFADIVHREQLWTGSIVSILDRQGVTIARTPHDERFVGKKVGPILMPRLLTESE
                     GVIETVSADGVPVIAAFSHVPVFGWAVTIGLPRAELIDTARRAAWLTFGVGAACLVLG
                     LALAQAMVRRVAAPIASLGALCVGLEQDDPDVSAATGLAETDQVAQALIAAASKRHAA
                     ETAARKAEAEREQGAALLRTVIESTPSLIFAKDCAGRMVLANGAFLAFLGKTWAEVAG
                     RTDLEFFDDRTEAKAVMENDRRIMAAGRAEACEELVGKDGNRPRVWLSTKTPLRNADR
                     EVIGLVAVSVEITERKQVEDRLRQMVNELNHRVKNTLATVQSIASHTLRGADRTFRQT
                     LEARLLALASAHDILTREGWEGANLTDVIAGALMPYGSADDDRFRVSGPPLRLQPRAA
                     VALAMALHELATNALKYGALSVAGGRVEIRWEVRHVAPAVLHIVWSEQRGPAVDRPGP
                     PGFGTSLIERSLAQDLGGTAQITFPVDGITCVIDAPLAEVLATSEILPFPLVGNM"
BASE COUNT          505 a          859 c          887 g          510 t
ORIGIN      
        1 ctcctgcaca gtaggcgttc cggtgaatat cagcagtttc cgcctagccg tcgctagcga
       61 aaaacgaaaa ggccacctgc ccaaccccaa ccggccttcg actgggacgc ggaccgcggg
      121 cattacaggc ggcaagagca gcaatgttgc cgatgtgacg gtcctgagcc atatccgagg
      181 gaagtgccgt tcgttccgaa tctccgaccg cgagagggcg ggcttactgt cggaatcgac
      241 attaaacctt gcaccaaacg attccatctg cttcacaatg ccggatgcta ggtcctcgtg
      301 tgccggaggg gcctgggcac aaggtgaaga tcgcaatgtc gccgcgggaa gtccctgatc
      361 aagttggcaa gccagggatt cacgcacgac ctgcgagcag cccgcgtctc cgcggacggc
      421 ttctcctgct ggtcgtggct tgcagcctgc cgttgttcgt cttcggcttc ggcaatgcgt
      481 atttcgacta ccaagctgat cgggccgagg ctggacgacg ggccttgaac atgacgcgca
      541 gccttgccct gctggtgcaa cgggacctgg aggcgcgcct cagtaccctc caggtgctgg
      601 cgatgtcaca ggcgctgcaa gctggcgata tcgccagttt ccgggtccgg gctgcggcgg
      661 tgctgcggct gcaactccct ggggcgaaca tcgtgctcct gcgagaggac ggccagcagg
      721 tgatgaacac cgcgctaccg gcgggtgcgg cgcttcctgc acggcgtgac aacacgtcgt
      781 tgcacctcct cttcgccacg gcacaaccat ccgtttccga catattcatc agcgtcgtcg
      841 atcggccgac cctcgcgctc gacgtgccgg tgcgtagccc cgacggcagc gtcctgtaca
      901 gcctcacgct caatccgacc cttgcggcgt tcgccgacat cgttcaccgc gaacaactgt
      961 ggaccggctc gatcgtctcg atcctggatc ggcaaggcgt cacgatcgca cgcaccccgc
     1021 acgacgagcg gttcgtgggt aagaaggtgg gtccaatcct gatgccgcgc ctgctcacgg
     1081 aatcagaagg cgttatcgag accgtgtcgg cggatggtgt gccggtaatc gctgccttca
     1141 gtcatgtgcc ggtcttcggc tgggctgtga cgatcggctt gcctcgggca gaacttatcg
     1201 acaccgccag gcgtgcggca tggctcactt tcggtgtcgg agcggcctgt ctggttcttg
     1261 gccttgcttt ggctcaggcg atggtgcggc gggtcgcggc cccgattgcc tcgcttggcg
     1321 ctctttgcgt tggcttggaa caggatgatc ctgacgtctc ggccgcaacg ggattagccg
     1381 agacggatca ggtcgctcag gcgctgatcg ccgcagcctc caaacgccac gcggccgaga
     1441 ccgccgcccg caaggccgag gcggagcgag agcaaggcgc ggcactgctg cgcaccgtca
     1501 tcgagtcgac gcccagcctc attttcgcca aggactgtgc cgggcgcatg gtgctggcaa
     1561 acggcgcttt tctggcattc ctcggcaaga cctgggccga ggtcgcgggg cgaaccgacc
     1621 ttgagttctt tgatgatcga acggaagcca aagccgtgat ggagaacgat cgccggatca
     1681 tggcagcggg ccgggccgaa gcgtgcgagg agttggtcgg caaggacggc aaccggccgc
     1741 gcgtgtggtt gtccacaaag accccgctgc gcaatgcgga tcgggaagtc atcgggttgg
     1801 tcgccgtatc ggtcgaaatc accgaacgca aacaggtcga ggaccggttg cgccagatgg
     1861 tcaacgaatt gaaccaccgg gtgaagaaca ccctggccac cgttcaatcg atcgcctcgc
     1921 acacacttcg cggtgccgac cggacatttc ggcaaacgtt ggaagcgcgg ctcctggcac
     1981 tggcatccgc ccacgacatc cttacccgcg aggggtggga aggggcaaac ctgaccgacg
     2041 tgatcgccgg tgcactgatg ccgtatggtt cggccgacga tgaccggttc cgggtttccg
     2101 gtccgccgct gcgcctgcag cctcgggccg cggtagcgct ggccatggcg ctgcacgaac
     2161 tggcgacaaa cgcgctgaag tacggcgctt tgtccgtcgc cggcggacga gtggaaatca
     2221 gatgggaggt caggcacgtt gccccggctg ttctgcacat cgtctggtcg gagcagcgcg
     2281 gtccggcggt cgatcgaccc gggccccccg gcttcggcac gagcctgatc gaacggagct
     2341 tggcacagga tctgggcggg acagctcaga tcacgtttcc agttgacggg atcacctgcg
     2401 tgatcgacgc acccctcgcc gaggtgctcg caacctccga gattttgcca ttcccgctgg
     2461 tcggcaacat gtaaggaagc caggcatgaa catgacaaca cgcgggaggc ggtccggaga
     2521 atgaccggcc ggaacctgct acttaactag tatagaaccg taccgccggt cgtcggctcg
     2581 gtggtcgatg ccacgatggt tcccatcatg ccctatttgg attgcggttg acgtgaacat
     2641 ccagcatcag gactaccttt cagcgcgcga aatcacgttg atacgcgaag tgccgcactt
     2701 caaggacagc agtgaggcac ggcacgatat cggcttgcat tatcggtctt atgcggccgg
     2761 a
//
LOCUS       DASWQN010000965         9655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2467077, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000965 DASWQN010000000
VERSION     DASWQN010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9655
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467077"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            500..1735
                     /locus_tag="VGC82_16495"
     CDS             500..1735
                     /locus_tag="VGC82_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY0424919.1"
                     /translation="MTIATVNNGDMIIMQKLSPKFEQANNIKIDWVVLEENTLRQKVT
                     TDIATKGGSFDVITIGAYETPIWGKRGWLVPVDDLGADYDYADILDPVKNGLSFGGKL
                     YAVPFYAESSFTYYRKDLFDKAGLKMPEQPTYDEIKTFADKLTDPKAGVYGICLRGKP
                     GWGENMAYLTTLVNANGGKWFDQKWQPQLTSPEWKQSLAFYVDLLKRDGPKGATANGH
                     NENRALFATGKCAMWIDATSAAGYIFNPKDSQVADKTWFAKSPTGSFKGGSGWAWAWG
                     LGIPTSSKHAADAKKFLKWATSKEYITMVGESQGWVAAPPGTRKSTYANPNYKKAAPF
                     ADVVLAAILAADPTKPTRDPVPYTGVQFVAIPEFQGIGTTVGQSVAAALAGSQTVDQA
                     LETAQAAAERTMKQAGYLK"
     gene            1754..2650
                     /locus_tag="VGC82_16500"
     CDS             1754..2650
                     /locus_tag="VGC82_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY0424920.1"
                     /translation="MATQATTPSPKGGGTAVRTLPLVGPSVGVLLIWMIVPLAMTLWF
                     SLQRYNLLDPAEKAFSGIENYKFLLTNPSLFTAMGHTLQLVGAVLVVSVVFGTLFAIL
                     LDQDFYGQGVARLFVIAPFFVMPTVSALIWKNLLMHPDNGLFAFITSSLGLGVVDWFT
                     EYPLTAIVIIVAWQWVPFATLILLTAIQSLSGEQVEAARMDGAKPLSMFWFIILPHLL
                     RPITVVVMIETIFLLTVFAEILVTTSGGPGDATTTLTYLIYLRALVEWDVGGASAGGM
                     IAIILANVMAFFLVRTVARNLD"
     gene            2799..3620
                     /locus_tag="VGC82_16505"
     CDS             2799..3620
                     /locus_tag="VGC82_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0424921.1"
                     /translation="MKNKQAKKWLMTVVAWLVAGAVFFPIFWMALTAFKTEIDAVSIP
                     PKLFFLPTFENYVAVSQRADYFKFAWNSVVISVGSTIASLLLAIPAAYAMSFFPTHRT
                     KDTLLWMLSTKMMPAVGVLVPIYIMFRDTGLLDTRTGLIILYTLSNLPIVVWMLYSFF
                     KELPKEILEAARMDGAGPFEQMLLLIMPLSAPGIASTGLLSIILCWNEAFWSLNLSAA
                     KAAPLTAFIASFSSPEGLFWAKLSAASTLAVLPILIFGWLTQKQLVRGLTFGAVK"
     gene            3683..4816
                     /gene="ugpC"
                     /locus_tag="VGC82_16510"
     CDS             3683..4816
                     /gene="ugpC"
                     /locus_tag="VGC82_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY0424922.1"
                     /translation="MATVEVKDLRKAYGETVIIKGINLRVEDREFAVFVGPSGCGKST
                     LLRMIAGLEEISGGELLIDGQISNDVPPDKRGCAMVFQSYALYPHMTVADNMGFALKL
                     AKIPKEERDRKVQEAAKILHLEPLLERTPKALSGGQRQRVAIGRAIVRNPKVFLFDEP
                     LSNLDAALRVQMRIELTRLHEELDATMIYVTHDQVEAMTMAEKIVVLQSGIIEQVGSP
                     LELYHHPRNQFVAGFIGSPKMNFLPGSIAALGPDGAVVALVGAVPITVPVKTEGLTIG
                     QKVTLGVRSEHLSVADDGQLKGEVLVAERLGGSTFLYTTVKNLPTLTIESGGENPARV
                     HDLLGIKIDAASCHLFTESGEAVPRAVRHPMADMKRSIGRRVD"
     gene            4991..5719
                     /locus_tag="VGC82_16515"
     CDS             4991..5719
                     /locus_tag="VGC82_16515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008854010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0424923.1"
                     /translation="MARELEGKVAVVTGAASGIGLASTEAMMAAGARVVLVDRDDAAL
                     AALCSKRGDAAIPLVIDLLDSKACATLVPRVLEMAGQIDILHANAGTYVGGDLVDADT
                     AAIDRMLNLNVNVVMKNVHDVLPHMIERRTGDIIVTSSLAAHFPTPWEPVYASSKWAV
                     NCFVQTVRRQVFKHGIRVGSVSPGPVISALLADWPEEKLREAKESGSLIEASEVAEVV
                     MFMLTRQRGMTIRDVIMMPSNFDL"
     gene            5779..6015
                     /locus_tag="VGC82_16520"
     CDS             5779..6015
                     /locus_tag="VGC82_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424924.1"
                     /translation="MQILTLGELLAEFMAGRVGQIFHRAGNFPWAYPSRAPAIFVDQA
                     AKTCASAAMFGCVGQDSFGDLILNRLRNDSVGRA"
     gene            6048..7550
                     /locus_tag="VGC82_16525"
     CDS             6048..7550
                     /locus_tag="VGC82_16525"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEY0424925.1"
                     /translation="MANTEPKPKAIKLNAAALKRLAPGIRVPTYQREGLPHSIVHIGV
                     GGFHRAHEAVYLDDLLHQPGNREWGMVGVGLLQHDARIRDAMRSQNCLYALVERGASG
                     ESARIIGSIIDFMFAPEGSEAVLEKMASPDCRIVSLTITEGGYYVNSGTGEFDADHPD
                     IKRDLANPGQPTCSFGYLVEALDRRRKRGLGPFTVMSCDNMQSNGDVTKKMLTAFAHL
                     RDPALGRWVADNVAFPNAMVDRITPATTDEHRALTREKFGIDDAWPVMTEPFKQWVIE
                     DHFNQGRPAWDKVGAQMVPDVLPYEKMKLRLLNASHQAMCYIGMLVGHTLAHETMEDP
                     RIRKLVQTMMDVEVTPHLPAVPGIDLAEYKKTLIERFANPAIRDQLARIGTEGSARIP
                     KFVLPSVIDALPRGAPVKLMAFTVAAWFRYLTGKDDNGNPMPINDPMEARITEAARRG
                     GADATAMLSLSELFGDELPKANPFSQEVARALKSFYEKGAKATLESYVGD"
     gene            7554..7682
                     /locus_tag="VGC82_16530"
     CDS             7554..7682
                     /locus_tag="VGC82_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424926.1"
                     /translation="MNFAFDRVFDLLGQAALITGGATGIGKAICNAVRGARFDRSK"
     gene            7847..8524
                     /locus_tag="VGC82_16535"
     CDS             7847..8524
                     /locus_tag="VGC82_16535"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEY0424927.1"
                     /translation="MLLVDTADNGLILEALKFPAVRGFTTNPTLMAHAAGAESLRISE
                     YIARARELCHLPKNQAVSHIDHLMIQAVGSREHMLEQAAIYSGELKTTSGTRLWIKLP
                     PTQQGIACCPALKKLGCATLVTAVYTATQALVAMEAGADGIAVYLGRMMRLEDDWEAQ
                     LERIAKVVSEAGKMLLLASFPDIQTVERALDYSRDLTVPPNVLQGILESPHSEDALKG
                     FDSKILK"
     gene            8853..>9655
                     /locus_tag="VGC82_16540"
     CDS             8853..>9655
                     /locus_tag="VGC82_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY0424928.1"
                     /translation="MYLGIDLGTSEVKVLLVDDDQRIAATGRAALTVQRPRALWSEQH
                     PQDWWEATQAAVAQIQKAAPQALAAVRGIGLSGQMHGATLLDSRDEVLRPAILWNDVR
                     SGEECRELEAQVPNSRELTGNLAMPGFTAPKLLWVAKHEPEVFARTAKVLLPKDYLRL
                     KLTGEYVSEMSDASGTLWLDVAGREWSDTMLAATRLSRANMPRLVEGSEASGQLLPEV
                     ARAWSMDRVVVAGGAGDNAASAIGMGAVNEGESFISLGTSGVFFVANNA"
BASE COUNT         1891 a         2866 c         3001 g         1897 t
ORIGIN      
        1 cgtgcgaaca tctccaaggg cgtgtggatg gcgtgtgtgg gtcgtggtct accagcaatc
       61 ggttgggacg accgcctctc cttcggcatg gtcagcatca caccccagga catcctatcg
      121 agcttgggca tcgagcgccc cgtctggcgg cgccccgcgc agcgcaccta agccgtctgc
      181 aaggcatagt ccattgcagc ccctgtattg cctccgagct tgcggctgat gctggggcgc
      241 atgcgtctca tgcatctcat gcatctcatg ctcgcaatcg tcatcctatc ctccggctga
      301 agctgctggt acttcgtttg ttcttgcgtg tgcaggcctt acgcgatgag gtgttgcatt
      361 tcagatttga ggccgccata ccgtaatagt cggtcgaatt gaccacttct caaggggata
      421 catcgtgtcg aagcctgttc gcattgcagc attcgtagct ctcacatgcg ctgccgcggt
      481 cgcatacgcg cagtccgagt tgactattgc cacagtcaac aacggcgaca tgatcataat
      541 gcagaagctg tcccccaagt tcgagcaggc taacaacatc aaaatcgact gggtggtgct
      601 tgaagaaaac acgctgcgac agaaggtgac gaccgacatc gctaccaagg gcggttcgtt
      661 cgatgtcatc accattggcg cctacgaaac gcccatctgg ggcaagaggg gctggctggt
      721 gccggtggat gatttgggcg cggactacga ctatgccgat atcctcgatc cggtaaagaa
      781 tggtctgtcg tttggcggca agctttacgc ggtgccgttc tacgccgaga gctcgttcac
      841 ctactaccgt aaggatctgt tcgacaaggc tggcctcaaa atgcccgagc agccgaccta
      901 cgacgagatc aagacattcg ccgacaagct gacggatccc aaggcgggag tctatggcat
      961 ttgcctgcgc ggcaagccgg gttggggcga gaatatggca tatttgacga ccctggtgaa
     1021 tgccaacggc ggcaagtggt tcgatcagaa atggcagccg cagttgactt cgcccgagtg
     1081 gaagcagtcc ctcgctttct acgtcgacct gctgaagcgc gatggaccaa agggcgcgac
     1141 tgccaacggt cacaacgaga accgcgcgct gttcgctacc ggcaagtgcg ccatgtggat
     1201 cgatgcgacc tccgccgccg gctacatctt caatccgaag gacagccagg tggccgacaa
     1261 gacctggttc gccaagtcgc cgacgggcag cttcaagggc ggctccggct gggcttgggc
     1321 gtgggggctg ggaattccga cctcttccaa gcacgcggcg gatgcgaaga agttcctgaa
     1381 atgggcaacg tccaaggagt acatcacgat ggtcggcgaa tcccagggct gggtcgccgc
     1441 gcctccggga acccgcaagt ccacctacgc caaccccaac tacaagaagg cggcgccgtt
     1501 cgctgacgtg gtgctcgccg ctattctcgc ggccgacccg accaagccca cgcgtgaccc
     1561 ggttccctat accggcgtgc agttcgtggc gatccccgag ttccagggca tcggcaccac
     1621 ggtcgggcag tccgtcgccg cggcgctcgc cggttcgcag acggtcgacc aggcgctcga
     1681 aaccgcccag gcggccgccg agcgaaccat gaagcaggca ggctacctca agtaacaggc
     1741 caggacacta gccatggcta ctcaagcgac aacgccgtcc ccaaaggggg gcggtacggc
     1801 ggtgcgcacc ctgccgttgg taggaccctc ggtcggggtc ctgctgatct ggatgatcgt
     1861 gccgctggcg atgacgctgt ggttctcgct acagcgctac aacctcctcg acccggccga
     1921 gaaggcattc tccgggatag agaactacaa gttcctgctc accaacccgt cgcttttcac
     1981 ggcgatgggg cacactctcc agttggtggg ggcggtgctg gtggtctcgg tggttttcgg
     2041 gacgctgttc gcgattctcc tcgaccagga cttctacggc cagggcgtgg cgcggttgtt
     2101 cgtgatcgcg ccgttcttcg tgatgcccac ggtgagcgcg ctgatctgga agaacctgct
     2161 catgcacccg gacaacggat tgttcgcgtt catcacgagc tcgctcgggt tgggggtggt
     2221 ggactggttt accgaatatc cactgaccgc gatcgttatc atcgtcgcct ggcagtgggt
     2281 gccgtttgcg acgctgatcc tgctcaccgc gatccagtcc ttgagcggcg agcaggtgga
     2341 agccgcgcgc atggatggtg ccaagccgct gtcgatgttc tggttcatca tcctgccgca
     2401 cctgttgcgc cccattacgg tggtggtgat gatcgagacg atctttctcc tcactgtatt
     2461 cgccgagatc ctggtcacga cttcgggagg gccgggcgat gcgacgacca cgctcaccta
     2521 tcttatctac ctgcgcgcgc tggtggaatg ggacgtcggc ggggcttcgg cgggcgggat
     2581 gatcgcgatc atcttggcca acgtgatggc cttcttcttg gtgcgcactg tggcgcgcaa
     2641 cctcgactag tggcctgtga cagtcggaga gcgatagaga gtatggctga ggcaaaaacg
     2701 gcgagagcaa gacgcgccga caagacaata gtcggcactc tggcgaggag gccagatgct
     2761 accgatctgg ctgttacagc tccactggtg aggcggccat gaagaacaag caggcaaaga
     2821 agtggctgat gacggtggtc gcgtggctgg ttgcgggtgc cgtgttcttc ccgatcttct
     2881 ggatggcgct cacggcattc aagaccgaga tagacgccgt ttccatcccg ccgaagctgt
     2941 ttttcttgcc caccttcgag aactatgtcg cggtcagcca acgggcagac tacttcaagt
     3001 tcgcttggaa cagcgtggtg atttccgtcg ggtcgactat cgcaagcctg ctgctggcga
     3061 tcccggccgc ctatgcgatg tcgttctttc cgacgcaccg gaccaaggac acactcctct
     3121 ggatgctctc caccaagatg atgccggcgg tgggcgtgct ggtgccgatt tacatcatgt
     3181 tccgcgatac cggtctgctg gacacgcgca ccggactgat catcctctat acgctttcca
     3241 acctgcccat cgtggtatgg atgctgtact cgtttttcaa ggaactaccg aaggaaatcc
     3301 tcgaggcggc acgcatggac ggcgccggac cgttcgagca gatgctgctc ctcatcatgc
     3361 cgctctccgc gcccgggatc gcatcgacgg ggttgctgtc gatcatccta tgctggaacg
     3421 aggcgttctg gagcctgaac ctctcggctg ccaaggccgc gcctttgacc gccttcatcg
     3481 cgtcgttctc gagcccggag ggcctcttct gggcaaagct ttcggcagcc tccacgctgg
     3541 cagtcttgcc gatcctgatc ttcggctggc taacgcagaa acaactggtg cgtggcctca
     3601 ccttcggcgc agtcaaatga ctcaagcgaa accgggcgta cggtacgacg tccgccgcca
     3661 cgtatagaaa aaaggcataa ccatggcaac cgttgaagtc aaggatctgc gcaaagccta
     3721 tggcgaaacc gtcatcatca agggcatcaa tctacgcgtc gaagaccgcg aattcgcagt
     3781 cttcgtcggt ccatccggct gcggcaagtc gactttgctg cgcatgatcg ccgggctaga
     3841 agagatcagc ggtggtgagc tgcttatcga tggccagatc tccaatgacg tgccgccgga
     3901 caagcgcggc tgcgccatgg tcttccagag ttatgcgcta tacccgcaca tgaccgtggc
     3961 cgacaacatg ggcttcgcgc tcaagctggc aaagattccg aaggaggagc gcgacaggaa
     4021 ggtgcaggag gctgcgaaga tcctgcacct cgagccgctc ctcgagcgca cccccaaggc
     4081 gctctctggc ggacagcggc agcgggttgc gatcggccgc gcgatcgtgc gcaaccccaa
     4141 ggtgttcctg ttcgatgaac cgctgtccaa tctggatgcc gcgctgcggg tgcagatgcg
     4201 catcgaactc acccggctgc atgaggagct cgacgcgacc atgatctacg tgacccacga
     4261 ccaggttgag gccatgacca tggccgagaa gatcgtggtg ctccagagcg gaattatcga
     4321 acaggtggga tcgccccttg agctttacca ccatccgcgc aatcagttcg tggccggctt
     4381 tatcggctcc cccaagatga atttcctccc cggttccata gccgcgctgg gtccggacgg
     4441 agcggtggtt gcactggtcg gcgcggtgcc aatcacggtt ccggtcaaga ccgagggcct
     4501 caccatagga caaaaggtca ccctcggcgt acggtcggag catctctcgg tcgcggatga
     4561 cggccagctc aaaggcgaag tactggtcgc cgaacgcctg gggggttcca cgttcttgta
     4621 caccaccgtc aagaaccttc ccacccttac tatcgaatcg ggcggcgaga acccggcgcg
     4681 cgtccacgat ctgttgggca tcaagattga tgctgcgtcc tgccatttgt tcaccgaatc
     4741 gggagaagcg gtgccgcgcg ctgtacgcca tccgatggcg gacatgaagc gctcgatcgg
     4801 acgacgcgtg gactgaactg ggattgattg tttgaacgtc cccttccctg tagggcagcg
     4861 ggttatcgaa attcgcacag tctcacagcg agagcagtcc actggaaaga cgcagggccg
     4921 tatcttccgg gcaggttaaa tccaggatta gatgcccgca agcgcgggcc aaccaagaag
     4981 gacagagagc atggcaagag aactggaagg caaggtcgct gttgtgactg gggctgcgtc
     5041 gggcattggg ctggccagca ccgaggcgat gatggcggcc ggcgctcgcg tggtgctggt
     5101 cgaccgtgac gacgcagcac tggcggcact ttgcagcaaa cgcggcgatg cggcgatccc
     5161 gctggtcatc gatctgctcg actcgaaggc ttgcgcgacg ctcgtaccgc gcgtcttgga
     5221 gatggcaggg cagatcgaca tcctgcatgc caatgcaggc acttacgtcg gcggcgatct
     5281 ggtcgacgcc gacacggcgg cgatcgaccg gatgctgaac ctcaacgtca acgtcgtgat
     5341 gaagaacgtc cacgatgtct tgccccacat gatcgagaga cggaccggcg acatcatcgt
     5401 gacgagttcc ttggccgccc actttccgac gccatgggag ccggtctatg cgtcgtccaa
     5461 atgggcggtc aactgctttg ttcagacagt gcggcgccaa gtcttcaaac acggaatccg
     5521 cgtcggctcc gtctcgcccg gcccggtcat tagcgcactg ctcgccgact ggccggagga
     5581 gaagctgaga gaggccaagg aatcgggcag cctgatcgag gccagcgagg tagccgaggt
     5641 ggtgatgttc atgttgacgc ggcaacgcgg catgaccatt cgcgacgtga tcatgatgcc
     5701 gagcaatttc gacctctagg gaagctctgc tcaacccgca tccagtaata gtagtgggcc
     5761 aagatgatga aaccgttggt gcaaatccta accctgggtg aactgctcgc cgagttcatg
     5821 gccggccgcg tcggccaaat tttccaccga gccgggaact ttccttgggc ctatcccagc
     5881 cgtgccccgg caatcttcgt cgatcaggcc gcgaagacct gcgcttcggc ggccatgttc
     5941 ggttgcgttg gacaggatag cttcggtgat ctgatcctga atcgcctgcg caatgactcc
     6001 gtcggtcggg cttagcacgg acgactcatc aagtggagga gacaatcatg gcaaacactg
     6061 aaccgaagcc caaagcaatc aagctgaatg cggcggcact gaagcgccta gcgcccggga
     6121 ttcgggtgcc gacctatcaa cgcgaggggc taccgcacag catcgtgcat atcggcgttg
     6181 gtggcttcca ccgggcccac gaggccgtct atctcgacga cctgctgcac cagccgggca
     6241 atcgcgagtg gggaatggtc ggagtcgggc tgctccagca cgacgcgcgc atccgcgacg
     6301 ccatgcgcag ccagaactgt ctctacgcgc tggtggagcg aggcgcgtcg ggggagagtg
     6361 cgcggatcat cggctcgatc atcgatttca tgttcgcccc ggaagggtcc gaggccgtgc
     6421 tggaaaagat ggcctcgccc gactgccgca tcgtatcgct caccatcacc gaaggtggct
     6481 attacgtgaa ctctggcact ggtgagttcg acgccgatca tcccgacatc aagcgggatc
     6541 tcgccaaccc gggccagccc acctgttcct tcggctatct ggtcgaggcg ttggatcgcc
     6601 gccgcaaacg cggcctcggg ccgttcacgg tgatgtcctg cgacaacatg cagtccaacg
     6661 gtgacgtcac caagaagatg ctcactgcct tcgcccatct gcgcgaccct gcgctcggcc
     6721 gctgggtcgc ggacaacgtc gcgttcccca atgcgatggt ggaccggatc actcccgcga
     6781 ccacggatga gcaccgcgcg cttacgcgcg agaagttcgg tattgacgac gcgtggccgg
     6841 tgatgactga gccgttcaag caatgggtga tcgaggacca cttcaaccag ggtcgcccgg
     6901 cctgggacaa ggtcggtgcg cagatggtgc ccgacgtcct gccctatgag aagatgaaac
     6961 ttcgtctgct caacgccagc caccaggcca tgtgctatat cggcatgctg gtcggccaca
     7021 cgctggcgca cgagaccatg gaggacccgc gcatccgcaa actggtgcag accatgatgg
     7081 atgtcgaggt cactccccac ctgccggccg tgccgggcat agatctggcg gaatacaaga
     7141 agacgctgat cgagcgcttt gccaacccgg ccatccgcga ccagctcgcg cgcattggca
     7201 ccgaaggctc ggcgcgcatc ccgaagttcg tgctgccttc ggtgatcgat gcgttgccac
     7261 gcggtgcgcc tgtaaagctc atggccttca ctgtggcggc gtggtttcga tatcttacgg
     7321 gtaaggacga caacggcaat cccatgccga tcaacgaccc gatggaagcc aggatcaccg
     7381 aggccgcccg ccgtggcggc gctgacgcca cggcgatgct ttcgctgagc gagttattcg
     7441 gcgatgagtt gccgaaggcc aatccgtttt cgcaggaagt cgcgcgcgcg ctcaagagct
     7501 tttacgaaaa gggcgccaag gcgacgctcg aaagctacgt cggcgattga ctcgtgaact
     7561 tcgccttcga cagggttttc gacctgctcg ggcaggccgc cctcatcaca ggcggtgcga
     7621 ccggcatcgg caaggcgatt tgcaacgctg ttcgcggagc gcggtttgat cgttccaaat
     7681 gagcgacgag caacgccgcg tctcgaattg ctcatctttg attgcgacgg agtgctggtc
     7741 gacagcgagg tcatcagttg ccggtgtgcc gcggaagcgt gcgactcgcc ggcgtgggta
     7801 tggacacggt tgttgcgtaa aggccgtaag tacgctggag ccagtcatgt tgcttgtaga
     7861 tactgccgat aatggtttga ttttggaggc gcttaagttt ccggctgtgc gggggtttac
     7921 tacgaacccg actttaatgg cgcatgcagc cggtgcggag tcgctcagga tttcagaata
     7981 tatcgcgcgt gcccgcgagc tctgtcattt accaaaaaac caggcggtca gtcatatcga
     8041 ccatttgatg attcaagccg ttggttctcg agagcatatg ttggaacagg cagccattta
     8101 ttccggcgag ctaaagacca caagcggcac acgactttgg ataaagttgc caccaactca
     8161 acagggtatt gcatgttgcc ccgccctcaa aaaattgggt tgcgcaacgc ttgtgactgc
     8221 agtttacaca gctacgcaag cccttgttgc catggaagcc ggggccgatg ggatagctgt
     8281 gtatctggga cggatgatgc gtctggaaga cgactgggag gcgcagcttg agcggattgc
     8341 caaagttgtc tccgaagcag gaaaaatgct gctcttggcg agttttcctg acattcagac
     8401 agtcgagcgt gcgcttgact atagtcgcga tctgacagtg ccccctaacg ttcttcaggg
     8461 aatactggaa tctccacact ccgaagacgc actaaagggg tttgatagca aaattctgaa
     8521 atgatgagtt ttgcaacctc ttcgtgcgcc aatgattcca agcttttggc tggtgcggcc
     8581 gagcgccgtg cggacacgcc atgccgtact gcgctggatt atggcgcgat cctgctgacc
     8641 ccgtgatgca cgagatacgg tgcgcgggca ttgacctgtg atgtgaggtc gttgttgtac
     8701 aaatggtaag ttatgagacg agctttgcgg ccaatggaat caacggcttg cgtgtctcat
     8761 aagtctgtct caattcgatg gcggtgttgc cggcgctcgt aaggacgtgg ttcgaaatgg
     8821 ccgtatgacg acaattgcgg gagcgagaat tcatgtatct cggcattgac cttggtacgt
     8881 ccgaagtgaa agtgctgctg gtcgacgatg accagcgcat tgctgccact ggccgcgccg
     8941 cgctgaccgt gcagcggccg cgcgcgctct ggtccgagca gcatccgcag gactggtggg
     9001 aggccacgca agccgctgtc gcgcaaattc agaaggccgc gccacaggcg ctggcggccg
     9061 tccgcggtat cgggctgtcg ggccagatgc acggcgcgac gctgctcgat tccagagacg
     9121 aggtactccg gcccgcaatc ctatggaacg atgtccgctc cggcgaagag tgccgcgagc
     9181 tcgaggcgca ggtgccgaac tcgcgcgagc tcaccggcaa tcttgccatg cccgggttca
     9241 ccgcacccaa gctgctctgg gtggccaagc acgagccgga ggtatttgcg cgaacggcga
     9301 aagtgctgct gccgaaagac tacctccggc tcaagctcac cggtgaatac gtctcggaga
     9361 tgtccgacgc ctctggcacg ctgtggctgg acgtcgccgg acgcgagtgg tcggacacga
     9421 tgctggcggc cacccggctt tcccgtgcaa acatgcccag gctcgtggag ggaagcgagg
     9481 cgagcgggca actgctgccg gaagtcgccc gggcctggag catggaccgg gtcgtggtgg
     9541 ccggcggcgc cggagacaac gccgccagtg ccatcggcat gggcgccgtg aacgaaggcg
     9601 agagcttcat ctcgctcggc acctccggcg tgtttttcgt cgccaacaat gccta
//
LOCUS       DASWQN010000966         3866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2335629, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000966 DASWQN010000000
VERSION     DASWQN010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2335629"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VGC82_16545"
     CDS             complement(<1..204)
                     /locus_tag="VGC82_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEY0424929.1"
                     /translation="MSSVRVSTPDRRDRLWLLNALALALLTRLGAAGEALGYDRHLKS
                     NTTKRRTHSLFRQGCLLYQLIPTM"
     gene            889..1749
                     /locus_tag="VGC82_16550"
     CDS             889..1749
                     /locus_tag="VGC82_16550"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY0424930.1"
                     /translation="MALGSRRVGRNWMACCPVHHDSTPSLSIRDGGNGRVPVHCFAGC
                     DGENVIEALRDHGLWRGRKEQGPVSLYDGQRVRDDGERMQAAMAVGKECIPATDTLAE
                     RSLRSRGIHLSPLRPSLRDLTKVRHAAGIFLPAMVAEVTNALDGTSMAIHRTFLAKDG
                     RGKADLNPQQKMLGPCAGGVVRLDEPADVLTVGEGIETCLSTMVATGYPAWAALSAPN
                     LGSLELPEDGRDVIIAADGDQPGEEAARRYAVRWQRQGRRVRIARPPKGMDFNDRLLP
                     FAPRAVGASQ"
     gene            1746..3857
                     /locus_tag="VGC82_16555"
     CDS             1746..3857
                     /locus_tag="VGC82_16555"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HEY0424931.1"
                     /translation="MSGEHDTKLNGSMSPILDAFNKADEMTRATDDRTEEQTGPDMSI
                     LQMGRREPPVFPLDILGPSWKAWVMDTAQAAACPVDYVVATLLAAASALIGNARWVQP
                     VPGWAEPPHLWCGAVGDSGEGKSPGADVLLGIVLPELESRMAEDHDRNLLQWDAEAAI
                     ATAEKKEWENAVSQAAKGKTKMPPPLTGTLRPKPQQPCLIQNDVTIEKVASLLAEAAP
                     KGLLIIRDELSGWLTGLNNYNNAGRPFWIESYGGRPFRVDRQKLDKPVVVDRLVVALS
                     GGTQPEKLAKMFQNADDGLLARIIWFWPNPLEFKLSRAVPDVFFAINALDRLRRLTLR
                     ARAQEGAKPEPIKVPLPVDTHEPMEQFAREMQQLQHSTGGLLRSAYGKARGLVARVSL
                     VLEYLWWCGGEADIPEPASISAKAFYAAAYMIADYTMPMAARVYGDAAAGPAARHATT
                     LARYVIKKKLSEVHVRTLQRVDKLPGLKDAQTIHRAIDELIEVGWLVRPAAKGTAGAQ
                     RKAYAVNPAVFNNPATIAKRSHSRWALDFHTLERDDKWDKSKSVSNVNDLGKNDAVTN
                     QDTSDKSGHHEDDAVQQDRFVPLVLKCHGGRSEQIAPPGQTENPQDTDNKENSSQVDG
                     GKPDEGRFVPFVTGFQGDENDGPADRALTNRESDNPEVDNQEAPQAATGALPAQRRPL
                     SLEQQIALQRTGQWRRQHAAE"
BASE COUNT          841 a         1092 c         1212 g          721 t
ORIGIN      
        1 catggtgggg atgagttggt agagcaggca gccctggcga aacagcgagt gggtgcgtcg
       61 cttggtggtg ttggacttga ggtgccggtc atagcctagc gcttcgcccg cggcgccgag
      121 ccgcgtcagc aaggcgaggg cgagcgcgtt gagcagccat aggcgatcgc gccggtccgg
      181 cgtgctgaca cggaccgagg acatccccag gccgaaacgt aggtccttgg tgtcggggaa
      241 ggtgcactcg atatcccata caagataccg ctgataattt gacaggatcc gacgaatccc
      301 cgagattcgg cctccaagcg ctatgcgcgg tggtgcgctg gcttggggtt ctgtcagggc
      361 tcgcgccaaa atcgccggga taagcaatat ggcaacaatt gcggcggccc tgtggtcata
      421 atgtgccgtg cctagcccga cgcacgcaag taagtttcaa aactcgttac gctggcgcca
      481 agcctgacag aaccgcccag tcgcttgccg gcggctttct tcgtaataaa cgcgggggca
      541 attcaggtga atcgtttcgg cgtaacacgc tgttctctcc gggttttcgc gggataacgg
      601 catcttatag cacttgctgg cgggcgtcgg cagggaaaaa gatagggata cgccgaaaaa
      661 catcctggac acggatcgaa attaggtgca taaaaaaacg gtcgaaccac ttgggaggtg
      721 gatgcgaccg ttagaaattg tgaacaatgt ggacggttgt cgccgaccca ccaatacccc
      781 tctacgagga tgcgtttgtt aatcaaaggg gttggcaggt caagcaggca attgttcacg
      841 aacagtgaag gttgaaccat gcgtgacatg gacgctgaag cgatcgctat ggcccttggc
      901 agccgtaggg tggggcgaaa ttggatggcg tgctgtccag tccatcacga cagtacgcca
      961 agcctgtcta tccgtgacgg tggtaacggc cgcgtgccgg tgcactgctt cgccggctgc
     1021 gatggcgaga atgtcatcga ggccctgcgt gatcacggct tatggcgcgg ccgcaaagag
     1081 caggggccgg tgtccttata cgacggacag cgggtccgag acgacggcga gcggatgcag
     1141 gccgctatgg ctgtagggaa ggagtgcatc ccggccaccg acacgctcgc cgagcgatcc
     1201 cttcgatcgc gcgggattca cctctcacca ctccgaccat cgctccgcga ccttacaaag
     1261 gtgcggcacg ccgctggcat cttcctgccc gccatggtgg cggaagtcac caatgcgttg
     1321 gacgggacat ccatggccat ccaccgcacc tttctggcca aggacgggcg cggaaaagct
     1381 gacctcaatc cgcaacagaa gatgctcggt ccttgtgccg gtggcgtggt tcggcttgac
     1441 gagcccgctg acgtgctcac ggttggcgaa ggcatcgaaa cctgcctttc caccatggtg
     1501 gccaccggat atcctgcatg ggccgcgctt tcggcgccaa atctaggcag cctcgaactg
     1561 cctgaagacg ggcgcgacgt gatcatagcc gccgatggtg accaacccgg cgaggaggcg
     1621 gcgcgacgat acgctgtgcg atggcagcgg cagggcaggc gggtgcgcat cgcgcgtccg
     1681 cccaaaggaa tggatttcaa tgaccggctc ttgccattcg cgcctcgcgc cgtgggagca
     1741 tcccaatgag cggcgaacac gacaccaagc tcaatgggag catgagcccg atccttgatg
     1801 ccttcaataa ggccgatgag atgacccgtg cgacggatga tcgaacggag gaacagaccg
     1861 gccctgacat gtcgatcttg cagatgggcc gccgggagcc gcctgtgttc ccgttggata
     1921 ttcttggtcc gtcttggaag gcgtgggtca tggacacggc acaagccgcc gcgtgtccag
     1981 tggattatgt ggttgccacc ttgctagcgg ctgcctccgc gctgatcggg aacgcgcgat
     2041 gggtccagcc cgtgccggga tgggcggaac ccccgcatct ctggtgcggt gcggtagggg
     2101 acagtggtga gggcaaaagt cccggcgctg acgtccttct gggcatcgtt ctgcccgaac
     2161 tcgaatcacg catggcggaa gatcacgaca ggaacctttt acaatgggat gcggaagctg
     2221 cgatagccac cgctgagaag aaagaatggg agaatgcggt cagtcaggcc gcgaagggaa
     2281 agacaaaaat gccgccgccg ctaaccggga cccttcggcc gaagccgcag cagccgtgcc
     2341 ttattcagaa cgacgtgacg atcgagaagg tcgcatcatt gctcgccgaa gccgcgccga
     2401 aagggttgtt gattatccgc gatgaactgt ccggatggtt aaccggcttg aacaactaca
     2461 acaacgcggg ccggcccttc tggattgagt cttatggtgg ccgtccgttt agggttgatc
     2521 gtcagaaact ggataaacct gttgtggtcg accggttggt ggtcgccttg tccggcggca
     2581 ctcagccgga aaagctggcc aagatgttcc aaaacgcgga cgatggctta cttgcccgga
     2641 tcatctggtt ttggcccaac ccacttgagt tcaagctttc ccgcgccgtg ccggacgtgt
     2701 tcttcgccat caatgctttg gaccgcctgc ggcgattgac attgcgagcg cgcgcgcaag
     2761 agggcgccaa gccggaaccc ataaaggtcc cactgcccgt tgatacccat gaaccaatgg
     2821 aacagttcgc gcgggagatg caacagctcc aacacagcac aggcggcctt ctgcggtccg
     2881 cctatggcaa ggcgcgcggc ttggtggcgc gggtgtccct ggtattggaa tacctgtggt
     2941 ggtgtggtgg tgaggccgac ataccggaac ccgcctcgat cagcgcgaag gctttttacg
     3001 cggccgctta catgatcgcg gactacacga tgcccatggc ggctcgggtt tacggtgacg
     3061 cggctgccgg accagcggca cgccatgcca ccaccctagc gcggtacgtc atcaagaaaa
     3121 agctgtccga ggttcatgtt cgcacgctgc aacgcgtcga taagctacct ggcctcaaag
     3181 acgcccaaac gatccaccgc gccattgatg aactgattga ggtcggctgg ttggtcagac
     3241 ctgccgcaaa gggcaccgcc ggcgcccagc ggaaagctta tgcggtcaac ccggccgtgt
     3301 tcaataatcc cgccacgatt gccaaacggt cacatagccg atgggcttta gatttccata
     3361 ccctagaaag ggatgacaaa tgggacaaat caaaatctgt gagcaatgtc aatgatttag
     3421 ggaaaaacga cgctgtgaca aatcaggaca cttcagacaa atcaggacac catgaagatg
     3481 atgcggtgca gcaagatcga tttgtcccat tggtcctgaa gtgtcatggc ggtagatcag
     3541 aacaaattgc gccgcctgga caaactgaaa atccgcaaga cactgataac aaagagaatt
     3601 cctcgcaggt tgatggcggc aagcctgacg aaggtcgatt tgtcccattt gtcacgggtt
     3661 ttcagggtga tgagaacgac gggccggcgg acagggctct taccaatcga gagagtgata
     3721 acccggaggt tgataatcag gaagcccccc aagccgccac cggcgccctg ccggcgcaaa
     3781 gacgcccttt gtcgctggag cagcaaatcg ccctccagcg gactgggcaa tggcggcgcc
     3841 agcatgccgc tgaatagcct gacgcc
//
LOCUS       DASWQN010000967         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1413126, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000967 DASWQN010000000
VERSION     DASWQN010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413126"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VGC82_16560"
     CDS             complement(<1..626)
                     /locus_tag="VGC82_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEY0424932.1"
                     /translation="MIIALSGGVGGAKLALGLSRIMPPEKLLVVVNTGDDFEHLGLSI
                     SPDIDTVAYTLSGLANRELGWGRHDETWSFMETMEALGGETWFRLGDRDLALHVERTR
                     RLKAGETLSAVTADLCRRMGVGPAVVPMTNDPVRTRLLTDLGWLDFQEYFVRRRCEPV
                     VSQLQFQGAGAARPHPTFIAALADPSLEAVIICPSNPFISVEPILAIPG"
     gene            complement(623..1384)
                     /gene="cofE"
                     /locus_tag="VGC82_16565"
     CDS             complement(623..1384)
                     /gene="cofE"
                     /locus_tag="VGC82_16565"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEY0424933.1"
                     /translation="MTARLELFAVPGLPMVREGDHLPDLILAGLQRAGQVLRDRDVLV
                     IAQKIVSKAEGRTVDLGDVVPSEQAIALAAKVGKDPRIVEVVLSESVKVVRSRPNLMI
                     MQHRLGFVMANAGVDQSNVAEADGRHRALLLPLDPDASAEAIRQALAPYADVGVVISD
                     SFGRPWRRGTAGVAIGSAGIPSVIDLRGKPDLFGRILEVSIIGFADEIAAAASLLQGQ
                     AAEAQPVVVVRGLTWQAPNVPVADVIRPPEEDLFQ"
     gene            complement(1496..>1790)
                     /locus_tag="VGC82_16570"
     CDS             complement(1496..>1790)
                     /locus_tag="VGC82_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F420-dependent glucose-6-phosphate
                     dehydrogenase"
                     /protein_id="HEY0424934.1"
                     /translation="RALEDTRIWAALALTPEEKVTVHNPKEMERLADALPIERAASRW
                     IVSSDPDEHIEKIKPYIDMGFRHLVFHAPGDDQARFLKLYGELVLPKLRQRFG"
BASE COUNT          335 a          592 c          574 g          289 t
ORIGIN      
        1 cccgggatcg ccaggatcgg ctcgacgctg atgaacgggt tggacggaca gatgatcacg
       61 gcctccagcg acggatcggc gagtgcggcg atgaacgtcg ggtgcggccg agcggcgccg
      121 gcaccctgga actgcagctg actgaccacc ggctcacacc gccggcgcac gaagtattcc
      181 tggaaatcca gccagccgag atcggtcagc agccgggtcc ggaccggatc gttggtcatt
      241 ggcacgacgg cgggacccac ccccatgcgg cggcacaaat ccgcggtcac cgcggacagc
      301 gtttcaccag ccttcaggcg acgcgtccgc tccacatgca aagccaggtc gcggtcgcca
      361 aggcggaacc aggtctcgcc gcccagcgcc tccatggtct ccatgaacga ccaggtttcg
      421 tcatgacggc cccagccgag ctcgcggttt gccaagccgg acaaggtata agcaacggta
      481 tcgatgtcgg gcgagatcga caggcccaga tgctcgaaat cgtcgccggt gttgacaacg
      541 accagaagct tctcgggcgg catgatgcga gacagaccga gcgccagttt cgcgccgcct
      601 actccaccgg acaaggcgat gatcactgaa aaagatcctc ttcaggcggg cgtatcacgt
      661 ccgccaccgg cacattcggt gcttgccacg tcaggccacg cacgacaacg acgggctgcg
      721 cctccgccgc ctggccctgc agcaacgagg cagcggcggc gatctcatcc gcgaagccga
      781 tgatgctgac ttccagaatg cggccgaaca ggtcaggctt gccgcgcagg tcgatgacgg
      841 aggggatacc ggccgaaccg atcgccacgc cggcggtgcc gcggcgccag ggccggccaa
      901 agctgtcgct gatgacgaca ccgacatcgg cgtacggagc cagcgcctgg cggattgcct
      961 cggccgacgc gtccggatcc agtggcagca gcaatgcgcg gtgccgtccg tcggcctccg
     1021 ccacgttcga ttgatcgacg ccggcgttgg ccatgacgaa acccagccgg tgctgcatga
     1081 tcatcaggtt tggccggctt cgcacgactt ttaccgattc cgatagcacg acttcgacaa
     1141 ttcgcgggtc cttgccgacc ttggccgcca gcgcgatcgc ctgttcggac ggtacgacat
     1201 caccgaggtc cacggtgcgg ccttcggctt tggagacgat tttctgcgcg atcaccaaca
     1261 cgtcacggtc gcgcagaacc tggccggcgc gctgcaggcc cgccagaatc agatcgggca
     1321 gatggtcgcc ttcccgcacc atcggcaggc ccggcacagc gaacagttca agacgggcgg
     1381 tcatttcggc acttggctcg cggaagccgg ccagcgcgaa acgacaacgc cgcgattgag
     1441 ccgcttgatg catttggggc caacgcctgc catgaggagg cacatcgtca gcgaactacc
     1501 cgaagcgctg gcgcagcttc ggcagcacca gttcgccgta gagtttcagg aaccgggcct
     1561 gatcatcgcc gggagcatga aacacgaggt ggcggaagcc catgtcgata tagggtttga
     1621 tcttctcgat gtgctcatcc gggtccgagg acacgatcca gcggcttgcc gcgcgttcga
     1681 tgggcagggc gtcggccagg cgctccatct ccttcgggtt gtgcacggtc accttttctt
     1741 ccggtgtcag cgccagggcg gcccagatgc gcgtgtcctc tagtgcgcgt
//
LOCUS       DASWQN010000968         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1676610, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000968 DASWQN010000000
VERSION     DASWQN010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676610"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VGC82_16575"
     CDS             complement(<1..497)
                     /locus_tag="VGC82_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424935.1"
                     /translation="MNKERLIPLIVATALFMENMDSTVIATSLPAIAADIGTSPLTLK
                     LAITSYLLSLAVFIPASGWTADRFGARMVFSLAIAVFMVGSVGCALSSSVTHFVIARI
                     VQGIGGAMMTPVGRLVLLRSIDKSALVNAMAWVTVPALVGPVIGPPLGGFITTYFSWH
                     WIFLI"
     gene            622..870
                     /locus_tag="VGC82_16580"
     CDS             622..870
                     /locus_tag="VGC82_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424936.1"
                     /translation="MIYILAALVPPLGLLMNGQPFAAIFNLVLIVFCLIFGLVFHILL
                     LVPSAHAVIAVHMKREQRRHREVVEAIRQHGPPPGYSR"
     gene            1042..>1697
                     /locus_tag="VGC82_16585"
     CDS             1042..>1697
                     /locus_tag="VGC82_16585"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY0424937.1"
                     /translation="MAVVQGLQGIREAYTFDDVLLKPGLSDILPSEADIRSRVTRAIG
                     LNIPIIASAMDTVTEARMAIAMAQAGGVGVIHRNFDPEGQAAQVRQVKKYESGMVVNP
                     LTIGPDAMLSDALTLMTDHGFSGIPVVTGAAKGVPGKLVGILTNRDVRFATDRRQKVS
                     ELMTHENLVTVREGVSQDEAKRMLHKHRIEKLLVVDDQYRCVGLITVKDMEKAVAHPL
                     A"
BASE COUNT          327 a          535 c          547 g          288 t
ORIGIN      
        1 ttgatcagga agatccagtg ccaggagaaa taggtggtga tgaaaccgcc gagcggcggc
       61 ccgatcacag gaccgaccag cgccggcacc gtgacccagg ccatcgcgtt gaccagcgcg
      121 ctcttgtcga tcgagcgcag caacaccagc cgtccgaccg gcgtcatcat cgcgccgccg
      181 atgccctgca cgatgcgggc gatgacgaaa tgggtaacgg aggaggacag cgcgcagccg
      241 accgacccca ccatgaagac cgcgatggca aggctgaaca ccatgcgggc gccgaagcgg
      301 tcggcggtcc agccgctcgc cggaatgaat accgccagcg acagcagata tgaggtgatc
      361 gcgagcttca acgtcagcgg gctggtgccg atatcggcgg cgatcgccgg cagcgaggtg
      421 gcgatgacag tggaatccat gttctccatg aacagggcgg tagccacgat gagcgggatc
      481 aggcgttctt tgttcatcga tttccgaagg gagaggtgga ttcgggcggg agtagcggct
      541 gtatcaccca gccgccatgg aggctattgc ggaacccggg ataccaccta aatcctgcgt
      601 gacgccgccg gagatccgat catgatctat atcctcgccg ccctggtgcc gccgctcgga
      661 ctgctgatga acgggcagcc tttcgcggcg atcttcaacc tggtcctgat cgtcttttgc
      721 ctgattttcg ggctggtttt tcacatcctg ctgctggtgc cctcggcaca tgcggtgatc
      781 gcggttcaca tgaaacgcga gcagcgccgg caccgggagg tggtggaggc gatccgccag
      841 cacggcccgc cgccgggtta ttcgcgctga agccttcatt tgaggccgcg gcccctttga
      901 atcatgccgg ggccatgcta tcgaccaccg ccaaccctac ccgatgggct ttgattcgac
      961 ggcgacgcca ggtgtgtcgc cgcaggacct gctcatcccg gggtcgtatc gcggcgaacg
     1021 ggccgccgaa tggagttggc aatggctgtt gtgcagggac tgcaggggat tcgcgaagcc
     1081 tatacgttcg acgacgtgct tctgaagccc ggcctgtcgg atatcctgcc ctccgaggcc
     1141 gatatccgct cccgcgtcac ccgggcgatt gggctcaaca ttcccataat cgcctccgcc
     1201 atggacaccg tcaccgaagc gcgaatggcg atcgcgatgg cgcaggccgg cggcgtcggc
     1261 gtgatccacc gcaatttcga tccggaaggc caggccgccc aggtgcggca ggtcaagaag
     1321 tatgaatccg gaatggtggt caatccgctg accatcggcc ccgacgccat gctgtcggac
     1381 gcgctgacgc tgatgaccga ccacggcttc tccggcattc ccgtggtcac cggcgcggcc
     1441 aagggcgtgc ccggcaagct ggtcggcatt ctcaccaacc gcgatgtgcg ctttgccacc
     1501 gaccgccgcc aaaaagtctc cgagctgatg acgcacgaaa acctcgtgac ggtgcgcgag
     1561 ggcgtcagcc aggacgaagc caagcggatg ctgcacaagc accgaatcga gaaactgctc
     1621 gtggtcgacg atcagtaccg ctgcgtcggc ctgatcaccg tcaaggacat ggaaaaggcg
     1681 gtggcgcatc cgctggc
//
LOCUS       DASWQN010000969         2761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1676612, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000969 DASWQN010000000
VERSION     DASWQN010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676612"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..1142
                     /locus_tag="VGC82_16590"
     CDS             6..1142
                     /locus_tag="VGC82_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0424938.1"
                     /translation="MNGMHYLDANASQPLRPAARSAVLAAFDVAGNPSSVHRAGRKVR
                     RIMEDARETIAACFGGDPANLVFSSGGTEADAAAIHAMSPGRRVIISAIEHDAVGSAA
                     AGAATIPVHPNGVVDLDALESQLSDGVASLVCLMLANNETGVIQPVEHASVICRRYGA
                     LLHVDAVQAAGRMAVHLDRLGAHSLAISSHKLGGPVGIGALLLAPDAPFARPLIGGGG
                     QERGRRGGTAPAALIAGFAAAAVAAGADDLAAISDLRDLAERAAVASGGIICGDRFRR
                     LGNTTCIALPGAAAGVQVIALDLEKVAVSAGAACSSGKVQTSHVLRAMDLGPLASQAI
                     RVSLPWNVTRADVDAFAAAYYRVSARLRTGIGGAADLDQHQAGG"
     gene            1221..2423
                     /locus_tag="VGC82_16595"
     CDS             1221..2423
                     /locus_tag="VGC82_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0424939.1"
                     /translation="MTESPPRPNQPVYLDNQATTRCDPRVVAAMLPFFSEDYGNPHSA
                     EHIMGQKAEAAVEAARVQVAGLIGAEAREVIFTSGATEANNIAIKGAARFAASMGNPR
                     RRIVTVATEHKCVLEAVADLAAEGFETVVLPVRGDGLLDPDALRDAVATPTLLVSVMA
                     VNNETGVIQDIQGLAAIVKEAGALFHSDMAQAAGKVPLDTTGWKVDLASISGHKLYGP
                     KGVGALFVRRRPRVRLLPLFSGGGQERGLRSGTLPTPLIVGLGEACRLAAMEMAVEAA
                     RIEHSRRGLFERLQQDIPGIRINGSVSARIAGNLNLTFPEATAADLMAALPDLCVSTG
                     SACSSAAVEPSYVLRALGLSDQEAARTLRVGIGRFTSAADIEYAAAALSAAHAACATD
                     HSESLHKA"
     gene            2429..2758
                     /locus_tag="VGC82_16600"
     CDS             2429..2758
                     /locus_tag="VGC82_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family 2Fe-2S iron-sulfur cluster
                     binding protein"
                     /protein_id="HEY0424940.1"
                     /translation="MPRMTFIERDGTRRDVDAPLGLSVLEIAHKHGVDIEGACEGSLA
                     CSTCHVIVDAGWFKKLETPTEDEEDMLDLAFGLTQTSRLGCQIVMNQDLDGLVVKLPA
                     GTRNAQR"
BASE COUNT          454 a          864 c          942 g          501 t
ORIGIN      
        1 ccgagatgaa tggaatgcac tacctcgatg ccaacgccag tcagccactg cgcccggcgg
       61 cgcgaagtgc tgttctggcc gcgttcgacg tggccggcaa tccctcatcg gttcatcggg
      121 ccggccgaaa ggttcggcgg atcatggagg atgcgcgcga gacgatcgcc gcttgcttcg
      181 gcggcgaccc cgcaaatctg gtctttagct cgggcggcac ggaggcggat gcggccgcaa
      241 ttcatgccat gtcccctggc cggcgggtca tcatcagcgc gattgagcac gatgccgtcg
      301 gctccgcggc agccggcgcc gccacgatac cggtgcaccc aaacggcgtc gtggatctgg
      361 atgcgctcga aagccagttg agcgacggcg ttgcttcgct ggtctgcctg atgctggcga
      421 acaatgaaac cggcgttatc caacctgtcg aacacgccag cgtgatttgc cgacggtacg
      481 gcgcgctgct gcacgtggat gccgtccagg cagccgggcg catggcggtc cacctggatc
      541 gactgggcgc ccacagtctt gcgatctcct cgcacaaatt gggcggaccg gttgggattg
      601 gtgccttgtt gctggcgccc gacgcaccgt tcgcgcgacc gttgatcggc ggcggtgggc
      661 aggagcgtgg ccgccgaggg gggaccgcac cggccgcgct aatcgccggt ttcgccgccg
      721 ccgccgtcgc cgccggagcc gatgatctgg ccgctatctc cgatttgcgg gacctggcgg
      781 agcgtgcggc cgtcgcaagt ggtggcatca tctgcggcga ccggttccgg aggttgggga
      841 acacaacgtg tattgccctg cccggcgccg cagcgggtgt ccaggtcatt gcgctggacc
      901 tggagaaagt cgccgtaagc gccggggccg cctgtagctc tggcaaggtg cagaccagcc
      961 atgtgcttcg cgcgatggat ctgggtccac tcgcgtccca ggcgatccgg gtttcactgc
     1021 catggaatgt gacccgagcc gatgtcgatg ccttcgccgc tgcttattat cgggtgtcgg
     1081 cacggctgcg cactggcatt ggaggcgcag cggacctgga tcaacaccag gccggaggct
     1141 gatttccact cttggggcct cgggccccac cgtgcgcctg gacgttccgc tcgggcgcaa
     1201 tcatgttcta ttggatggcc atgaccgaat ctccaccacg ccccaatcaa ccagtctacc
     1261 tggataatca ggccaccacg cgctgcgacc ccagagtcgt agcagcgatg ctgccgttct
     1321 tctccgagga ttacggtaac ccgcacagcg ccgaacacat tatgggacag aaggccgaag
     1381 ccgcggtcga agcggcgcgc gtgcaggttg ccgggttgat cggggctgaa gcccgagagg
     1441 tcatttttac gtccggcgcc accgaggcca ataatattgc catcaagggc gccgcgcggt
     1501 ttgccgccag catggggaat ccgcgccgcc gcattgttac ggtcgcgaca gagcacaaat
     1561 gcgtactgga ggcagtggcc gatctggcgg cggagggttt cgagacggtg gtgctgccgg
     1621 ttcgtggcga tgggctgctc gatcccgatg cgctgcgaga cgcggtggcg acgccgacat
     1681 tgttggtcag cgtgatggcg gtgaacaacg agaccggcgt gatccaggac atccagggtt
     1741 tggcggcgat cgtgaaggaa gcgggcgcgc tgttccactc cgacatggcc caggcagcgg
     1801 gtaaggtccc gctcgacacg acaggctgga aggtcgatct tgcgtccatc agcgggcaca
     1861 aactatacgg accgaaaggg gtcggggcgc ttttcgtccg ccgccgcccc cgcgttcggc
     1921 tgctgccact gttttccggc ggcgggcagg aaagggggct gcggtccggt acgctgccga
     1981 cgccgctgat cgtcggcctg ggggaggctt gccgcctggc ggccatggag atggcggtgg
     2041 aagccgcccg gatcgagcac tcgcggcgtg ggctgttcga gcggttgcag caggatatcc
     2101 ctggcattcg gatcaacggc agcgtttccg cgcggatcgc tggcaacctc aatctgacct
     2161 ttcccgaagc gaccgccgcc gacctgatgg cggcgttacc ggatctatgc gtgtccaccg
     2221 ggtcggcctg ttcgtcggcc gcagtggagc catcctacgt tttgcgtgcc ctgggacttt
     2281 ccgatcagga ggccgcgcga accttgcgtg tcgggatcgg acgctttacc tccgcagccg
     2341 atatcgagta cgccgccgcc gcactgtccg cggcccatgc cgcatgcgcc acggaccact
     2401 ctgagtcgct acacaaggcc tgatccgcat gcctcgaatg acatttatcg aacgcgatgg
     2461 cacacgtcgt gacgttgacg ccccactggg cctttcggtg ctggagatcg cccacaagca
     2521 cggggtcgat atcgagggcg cgtgcgaggg atcgctggct tgctcgacct gccacgtcat
     2581 cgtcgatgcc ggctggttca agaagctgga aacgcccacc gaggacgagg aggatatgct
     2641 ggacctggcg tttggcctga cccagacctc gcggctgggg tgtcagatcg tgatgaacca
     2701 ggatttggac ggtctggtcg tgaagttgcc tgccggcacc cgaaacgcgc agcgctgatc
     2761 a
//
LOCUS       DASWQN010000970         1710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2467669, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000970 DASWQN010000000
VERSION     DASWQN010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467669"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VGC82_16605"
     CDS             <1..569
                     /locus_tag="VGC82_16605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424941.1"
                     /translation="GGLVLGAMSVTWALASFVAGRIMMRTSYRLVAILGGLSLSIGCT
                     MLLALGPDDGPLWATMGSLVIGIGMGFCSTVFIVSIQASVPWRQRGAATSSSMFLRFV
                     GQSVGASGCGAVLNASMMYLDPGAVHAVDRLLDPAARRAMPVAEVLHLTEVMSGSLHN
                     AYLLATGFAVLTLLIAFRMPARLSPTRR"
     gene            601..1563
                     /locus_tag="VGC82_16610"
     CDS             601..1563
                     /locus_tag="VGC82_16610"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0424942.1"
                     /translation="MPAKPLVIALEEHFQHPEVGATYGPLDANRAPPMVQRLEDLGAG
                     RIQEMDQAGIDIQVISHTAPAMQKMTDAASAVRLARIANDHLHQAVRAHPDRFAGFAS
                     LPTPDPQAAADELERAVTQLGFKGAMIHGLTNGRFHDDKAFWPIFERAQALDVPIYFH
                     PSVPNPAVVDAYYKDYLEAVPGILRAAWGFTVETATQGVRLVLSGVFDKYPRLKVIFG
                     HLGEGLPFYAWRINMALGREWKASRSFRDVFSEHFYITTSGFWSDPALLCCIQELGLD
                     RIMFSVDYPFAPNQPATEWLHSVPLSSEDRIKLAGGNAQRLLKL"
BASE COUNT          277 a          560 c          536 g          337 t
ORIGIN      
        1 ctggcggcct ggtcttgggc gcgatgtcgg tgacctgggc attggccagt tttgtcgccg
       61 gccggatcat gatgcgcacc agctaccgcc ttgtcgcgat cctcggtggg ttatcccttt
      121 caatcggctg caccatgctg ctggcgctcg gccccgacga cgggccgctt tgggcgacga
      181 tgggctcgct ggtgattggt atcggtatgg gattttgcag caccgtcttc atcgtatcga
      241 tccaggcctc ggttccctgg cgtcagcggg gagcggcgac gtcgtcgtcg atgtttctgc
      301 gcttcgtggg ccaatcggtc ggcgcgtccg gatgcggcgc ggtattgaac gcctcgatga
      361 tgtatctcga tcccggcgcg gtgcatgcgg ttgaccgcct gctggacccg gccgctcgcc
      421 gcgccatgcc ggtggccgag gttctccacc tgaccgaggt catgtcggga tcgctccaca
      481 acgcatatct gctggccacc ggcttcgcgg tgcttacgct gctgatcgcc ttccggatgc
      541 ccgcgcgcct gagcccgacg cggcgatagt cagtcgaaaa caatcaggag gaagaaacca
      601 atgccggcaa aaccactggt catcgcactg gaagagcact tccagcaccc cgaagtcgga
      661 gccacctatg gtccgctcga tgccaaccgg gcgccgccga tggtgcagcg tctggaggat
      721 ctgggcgctg ggcggatcca ggagatggac caggccggca tcgacatcca ggtaatctcc
      781 cataccgcac cggcgatgca gaagatgact gacgctgcca gcgcggtgcg cctggcccgt
      841 atcgccaacg accacctgca tcaggccgtc cgcgcccatc ccgatcgctt cgccggcttc
      901 gcctcgctgc cgacgcccga tccccaggcc gccgccgacg aactcgaacg cgccgtgacg
      961 caactggggt ttaaaggcgc gatgatccat ggtttgacca acggccgttt ccacgatgac
     1021 aaggcgtttt ggccgatctt cgagcgggcc caggcgctgg acgtgccgat ctactttcat
     1081 ccctcggtgc cgaacccggc agtggtcgat gcctattata aggactatct cgaagccgtg
     1141 cccggcattc tgcgtgctgc ctggggcttc acggtggaga ccgcgaccca aggcgtgcgc
     1201 ctggtcctca gcggcgtatt cgacaagtac ccccgcctga aagtcatctt cggccacctt
     1261 ggggagggcc tgccgttcta cgcctggcgc atcaacatgg cgctgggccg cgagtggaaa
     1321 gcgagccgca gcttccgcga cgtgttcagc gaacatttct acatcaccac cagtggtttc
     1381 tggtccgacc ccgcgctgtt gtgctgcatc caggaacttg gcctggaccg catcatgttc
     1441 tcggtcgatt acccgttcgc gccaaaccag ccggcgaccg agtggctgca cagtgttccg
     1501 ctatcctcgg aggatcgcat caagctggcc ggcggcaatg cgcagcggct gcttaagctg
     1561 tagtcaagac gtctgccacg gatcgatcaa agtgacgccg cagctcagga agtccttggt
     1621 gttgcgggtg gcgatcgctt ccatgccgta agcccgggcg gtcgccgcga ttatggcgtc
     1681 ttcaaccgcg atcggcctgc cgtcggcctc
//
LOCUS       DASWQN010000971         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2467717, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000971 DASWQN010000000
VERSION     DASWQN010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467717"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VGC82_16615"
     CDS             <1..500
                     /locus_tag="VGC82_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEY0424943.1"
                     /translation="LAKLARQTGARIVVPTGALLGLDAVRAAAEGPIESISIETRKPP
                     MGLIGAPYLEKHGIDVMDLIQPLLVFEGNALDAAAGFPANVNVAAALALAGIGPIRTQ
                     VRIWADPGISRNIHTITVEAEAARFTMTIENVPSVENPKTGKLTALSLLACLRGLVSP
                     LRAGS"
     gene            505..831
                     /locus_tag="VGC82_16620"
     CDS             505..831
                     /locus_tag="VGC82_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424944.1"
                     /translation="MQEALVYLLTLMLFSVVIVAAARMKHEMLLLACIPPVFVALIVG
                     FIMALYAGDAFGALPTGAALLLGAPVGWWLAERFRSRDLLIAIYLAWAMAMVLALVAF
                     GFPDRV"
     gene            941..1687
                     /locus_tag="VGC82_16625"
     CDS             941..1687
                     /locus_tag="VGC82_16625"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0424945.1"
                     /translation="MTRQDLRHQGEAMLRTLFGSTAQPAGLANLLTEAAYGGIWSRPG
                     LPVQDRLVCSIAALGVGPRLKALRRHIGAGLDHGLSAEAIREILVQASLYAGFSAAEE
                     SLALAGEVFAERSIQFPADPPEDVSLEELDRRGAALMAVLHGDRANQGYASPDNPVTG
                     QLYPAAIRYGYGEIWSRPGLEHRQRALVAVAAFTALRLPEQVAKFGQSALNLGLTKTQ
                     VIEAIMQTAPLTGFPPALNALAAIGAALAA"
BASE COUNT          297 a          540 c          584 g          356 t
ORIGIN      
        1 atctggccaa gttggcccga cagaccggcg cccgcatcgt tgtcccaacc ggcgcgttgc
       61 tgggcctgga tgccgtgcgc gccgcggccg aggggccgat cgaaagcatc tccatcgaaa
      121 cccggaagcc gccgatgggc ctgatcggcg caccatacct ggaaaagcac ggcatcgacg
      181 tcatggacct gatccagccg ctgctggtgt tcgagggcaa tgcgctggac gccgccgccg
      241 gcttcccggc aaatgtgaat gtcgccgccg ccctggcctt ggcgggtatc ggacccatcc
      301 ggacgcaagt gcggatttgg gccgaccccg ggatttcccg caatatccac actatcactg
      361 tcgaagccga ggcagcacgt ttcactatga ccatagaaaa cgttccgtcc gtcgagaacc
      421 caaagaccgg caaactgaca gcactgtcgc tgctggcgtg tttacgcggt cttgtttcgc
      481 cgctacgggc cggtagctga gggtgtgcaa gaggctctcg tctatctgct gaccttgatg
      541 ctgttcagtg tcgtgatcgt ggccgccgcg cggatgaagc acgagatgct gctgctggcc
      601 tgcataccac cggtttttgt cgcactgatc gtcgggttca tcatggcgct gtacgctggc
      661 gatgccttcg gcgcgttgcc aaccggtgcg gcccttctgc tcggcgcacc ggttggatgg
      721 tggctggcgg agcggttccg atcaagggat ctgctgatcg cgatctacct tgcctgggcc
      781 atggccatgg tgttggcact ggtggcattc gggtttcctg atcgagtttg acaggggacg
      841 ttcggcacat cggccagcgc ggttttggca gatatgcatt gtccaactgg actctccgac
      901 tggttcggcg aaacttcgag aaccccgaac ggaggaaccg atgacccgtc aagacctgcg
      961 ccatcagggc gaagccatgc tgcggacgct gtttggatca acggctcagc cggctggact
     1021 cgccaatctg ctgaccgagg ccgcctatgg cgggatatgg tcgcggcctg gcctgccggt
     1081 gcaagatcgt cttgtctgct cgatcgcggc cctcggggtc ggcccccggc tgaaggcgct
     1141 gcgccggcac atcggcgccg ggcttgacca cgggctttcc gctgaagcga tccgggaaat
     1201 cctggtgcag gcgtcgttgt atgccgggtt ctccgctgca gaggaatcgc tggcgctggc
     1261 gggtgaagtg tttgccgaac gaagtatcca atttcccgcc gatccgccgg aagatgtatc
     1321 gctggaagag ctggaccggc gcggcgccgc attgatggcg gtgttgcatg gcgatcgagc
     1381 caatcagggt tatgcctcgc cggacaatcc ggtgacgggg caattgtatc cggctgcaat
     1441 ccgctacggg tatggcgaga tctggtcgcg gccggggctg gagcatcgcc agcgggcgct
     1501 ggtcgccgtg gccgccttca ccgctctgcg gctgccggag caggtggcca aattcggcca
     1561 atccgctttg aaccttggcc tgacgaagac gcaggtgatc gaggcgatca tgcagacagc
     1621 cccattaacc ggatttccac cagcattgaa cgcgctggcg gcgattggtg cggcgttagc
     1681 cgcgtgaccg gcacgttttt cgtgactgct gcgccagctt cgtctgagag gtggctagta
     1741 caccccgccg gagatcccaa cccgctttgg caagcta
//
LOCUS       DASWQN010000972         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2601267, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000972 DASWQN010000000
VERSION     DASWQN010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2601267"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VGC82_16630"
     CDS             <1..632
                     /locus_tag="VGC82_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HEY0424946.1"
                     /translation="RLAKSTETPLIFADVADNPGGGGRGNTMAILQAFHAAGVRNALV
                     GVIHDPALAAEAHALGVGTALQARFNRDGGDEFSTPFTASAVVAGLLDQPIRGRRGIY
                     ANNTLDIGKSAALTLNGITVIVLTHRYQCADPMFFEAFGIDVAAARVVVVKSRGHFRG
                     GFDEFFRHDQVIEVDAPGLTSPILSRFAWRHMPRPVLPIDATATWTPSP"
     gene            629..1729
                     /locus_tag="VGC82_16635"
     CDS             629..1729
                     /locus_tag="VGC82_16635"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY0424947.1"
                     /translation="MKLVDLPTPCLVLDRGILNRNIRVMAAALSRLKVPLRPHMKTAK
                     SIEVARLAMAGQPGGITVSTLAEAEYFFGHGITDILYAVGITPQKLEQVCKLNAEGAR
                     IIAITDDATMAGIIAAQPVPPRTLIEIDSGEERGGVAPDSDTLLEIAARLGRCLAGVM
                     THAGHSYAGRGVREIARIAEAERAAVTLAAGRLREAGHEVEIVSAGSSPTARHAENFA
                     GVTEIRAGVYMFGDLFQSEIETHGADAIAVTVLTSVIGRRPGRILVDAGGLALSKDRS
                     TEATAHDYGFGLALDIDGKHGLGNAIVRKAYQEHGVIELDAGHPIEPPPIGAKLRIAP
                     NHTCMTAAAHDRYFVVDGGQDVAAIWHRVNGW"
     gene            1862..2326
                     /locus_tag="VGC82_16640"
     CDS             1862..2326
                     /locus_tag="VGC82_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEY0424948.1"
                     /translation="MSPALALVGFVGLCLLVGAVGGSMTARAVQTWYTTLRAPPGTPP
                     NWVFAPVWATLYVTVGVAGWLVWKRLGAARPLRLWGWQLAANALWAPAFFGLHSPSLA
                     MSVMVVLLLLLGLTIRAFGGVHRPAAALMVPYGAWCLYAAYLNAGFLLLNHT"
     gene            2508..>2814
                     /locus_tag="VGC82_16645"
     CDS             2508..>2814
                     /locus_tag="VGC82_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HEY0424949.1"
                     /translation="MNAWWRAANYLSIGQIYLLANALLREPLRIEHVKPRLLGHWGTT
                     PGLNFLYVHLNRIIVERDLNLIFIAGPGHGAPGVVASTWLEGTYSETYPNVSRDAEGL
                     "
BASE COUNT          472 a          923 c          897 g          522 t
ORIGIN      
        1 ttcggttggc gaaatcgacc gagacaccgc tgatcttcgc cgacgtggcc gacaatcccg
       61 gcggcggcgg ccgaggcaac acgatggcga tcctgcaggc cttccatgcc gccggtgttc
      121 ggaacgcctt ggttggcgtc attcatgatc ctgccttggc ggccgaagcc catgccctgg
      181 gcgtcggcac cgccctccaa gcgcggttca accgcgacgg cggagacgaa ttctccacac
      241 ctttcactgc gtccgccgtg gtcgccgggc ttctggacca accgatccgt gggcggcgtg
      301 gcatctatgc caacaacacg ctcgacatcg gcaagtccgc cgccctgacg ctgaacggca
      361 tcaccgttat cgtcctgacc caccgctacc agtgcgccga cccgatgttt ttcgaggctt
      421 tcggcatcga cgtcgccgcc gcccgcgtcg tggtggtgaa atcccgcggc catttccgtg
      481 gcggcttcga cgaattcttc cgccatgatc aggtcatcga ggtcgatgcg cccggcctta
      541 ccagcccgat cctgtcccgg ttcgcatggc gccacatgcc tcgcccggtt ttgccgatcg
      601 acgccaccgc cacctggacg ccatctccat gaagcttgtc gatctgccga ctccctgcct
      661 ggtgctggac cgtggcatcc tgaaccgcaa catccgcgtc atggccgctg cgctgtcccg
      721 gctgaaggtg ccgctgcggc cacacatgaa gaccgccaag tcgatcgagg tggcgcgcct
      781 tgcgatggcc ggacagcctg gcggcatcac cgtctcgacc ctggcggagg ctgagtattt
      841 cttcggccac ggcatcaccg acatcctgta cgccgtgggg atcacgccgc agaagctgga
      901 gcaggtgtgc aagctgaacg ccgaaggcgc tcggattatc gcgatcaccg acgacgcgac
      961 aatggccggc atcatcgccg cgcagcccgt gccgccacgc acgctgatcg agatcgacag
     1021 cggcgaggaa cgcggcggag tggcgccgga cagcgatacg ctgctggaaa tcgcggcgcg
     1081 cctcggtcgc tgcctggcgg gtgtcatgac acatgccgga cattcctacg ccggacgagg
     1141 cgtgcgggag atcgctcgca tcgccgaagc cgagcgcgcg gcggtgaccc tggcggcggg
     1201 tcgtttgcgc gaagccgggc atgaagtgga aatcgtctcg gctggcagtt cgcccacggc
     1261 gcgacacgcc gagaacttcg cgggcgtcac ggaaatccga gctggcgtct atatgttcgg
     1321 cgatctgttc cagtccgaga tcgagactca cggcgccgac gccatcgcgg tcacggtgct
     1381 gaccagcgtc atcggccgcc gtcccggccg catcctggtc gatgccggtg ggctggcgct
     1441 atcgaaggac cgcagtaccg aggccacggc gcacgattac ggtttcggcc tcgccctgga
     1501 tatcgatgga aaacacggtt tgggaaacgc gatcgtgcgc aaggcttatc aggaacatgg
     1561 cgtcatcgaa ctcgacgccg gccacccgat cgagccaccg ccgatcggcg ccaagctgcg
     1621 tatcgcaccc aatcacactt gtatgactgc cgcggcccat gaccggtatt ttgtggttga
     1681 tggagggcag gacgtggccg cgatatggca ccgggtaaat ggctggtgaa cttgcggctt
     1741 tcacacccca tgccaatgtg tctaaaacgt ggagaaaatc aggcttgatt tagaaaggag
     1801 ccgtgccttc cagcagtctg gtcccaatga ctggggttcg tttccgggct cgtccgcgcc
     1861 ggtgtcaccc gcgcttgcgc tggttggatt cgttggcctg tgcctgctgg tcggcgccgt
     1921 cggtgggtcg atgaccgcac gggcggtgca gacctggtac accacgctgc gggcacctcc
     1981 cggcacgcca cccaactggg ttttcgcgcc ggtctgggca acgctttacg tcacggttgg
     2041 ggttgccgga tggctggtct ggaagcggct tggcgcggcg cggccgctgc gcctatgggg
     2101 ctggcagctt gccgccaacg cattgtgggc accggctttt tttgggctgc acagtccgtc
     2161 cttggcaatg agcgtgatgg tggtcctgct gctcctgctg ggtcttacca tccgggcatt
     2221 cgggggggtt catcgccccg cggccgcgct gatggtgccc tatggcgcgt ggtgcctgta
     2281 cgccgcttac ctcaatgccg gctttctgct gctcaaccac acttgatccc gcgcaacgct
     2341 gattagtccc cgatccgacg agaagggttt atcggatagc gttaagtccg cagacggacc
     2401 ggagcgcagg catgacggat cagcaagatc gcagacccca agaccgcaga ccccaaaatc
     2461 gcgggcccca agatcgcgag cccttggcca ccgaacaact ggagcgcatg aacgcctggt
     2521 ggcgcgcggc gaattatctg tccatcggcc agatctacct gttggcgaac gcgctgctgc
     2581 gagagccgct ccgcatcgag cacgtcaagc ctcgtctgct cggtcattgg ggcacgacgc
     2641 cagggctgaa tttcctctac gtccacctga accggatcat cgtcgagcgc gatttgaacc
     2701 tgattttcat cgccggaccg ggacacggcg cccccggcgt ggtggccagt acctggttgg
     2761 aaggcaccta cagcgagacc tatccgaacg tgtcccggga tgccgagggc ctgg
//
LOCUS       DASWQN010000973         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1281500, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000973 DASWQN010000000
VERSION     DASWQN010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281500"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..814)
                     /locus_tag="VGC82_16650"
     CDS             complement(125..814)
                     /locus_tag="VGC82_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY0424950.1"
                     /translation="MASGEFVAYYRVSTQRQGRSGLGIDAQKKAVLDFLDGGRWSLVA
                     AFEEHETGRDNDRPKLREAMALCRVRNATLVIARIDRLSRNAHFLLGLQEAKVRFVAA
                     DMPDANELTVGIMAVIAQAESKRISANTKAAMAAARARGRQLGHRDNLSAVGRAKGRP
                     LALAAKTIKAKARAVDLTPTIREIQGSGQTSSRHIAGELNTRGIATARGKQWTSVQVM
                     RLLARIGQNSG"
     gene            complement(910..1578)
                     /locus_tag="VGC82_16655"
     CDS             complement(910..1578)
                     /locus_tag="VGC82_16655"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0424951.1"
                     /translation="MIVSATDISPGAMLAETAEQQFRDAVRALHAQGDWYPMERSDTA
                     GLITLAQAADLIIIGQVNPHARLGPAWRPEEIVLACGRPVLMIPYIGSFAAIGRRVLI
                     AWDGSREAVRALNDALPLIGAAEIVTVMAVHSRERDFERDRLPMARIVGHLARHGIAA
                     HAVETVRTNIAISDVLLSSAADLSADLIVAGAYHHSPLREILTGGVSRELFQHMTVPV
                     LMSH"
BASE COUNT          366 a          651 c          623 g          405 t
ORIGIN      
        1 aaatcctatt tcccatacaa cagcgccttt tgccctctcg accagtgcat aggtgccggc
       61 cttcaggtcc cgaatcatct tacgccttcg cttcatctcg accgtgcggg cgccgtgctc
      121 cccatcaccc agagttctgg ccgatcctcg ccagcagtct catcacctgc accgatgtcc
      181 actgcttgcc tcgggccgtc gcgattcccc gtgtgttgag ttcgccggcg atgtggcgcg
      241 acgacgtttg acctgatcct tggatctccc ggatcgtcgg cgtgaggtcg accgctcggg
      301 cttttgcttt aatggtcttt gccgccagtg ccagtgggcg gcccttggcg cgccccaccg
      361 ccgagagatt gtctctgtgt cccagctggc gcccgcgggc ccgggcggcg gccatggcgg
      421 ccttggtgtt ggcgctgatg cgcttgctct cggcctgggc gatcaccgcc atgatgccca
      481 ccgtcagctc gttggcatcc ggcatgtcag cagccacaaa gcggaccttc gcctcctgca
      541 ggccgagcaa aaaatgggcg tttcggctca gccggtcgat ccgcgcaatc accagggtgg
      601 cattgcgcac gcggcacagc gccatcgcct cgcgtagttt cgggcggtcg ttatcgcggc
      661 cggtctcgtg ttcctcgaac gccgccacaa gcgaccagcg gccgccgtcg agaaagtcga
      721 gcacggcctt tttctgggcg tcgatgccga gcccgctgcg gccttggcgt tgagtggaaa
      781 cgcggtaata ggcgacgaat tcaccgctgg ccatggctgc tacgtttcct gcatgcttcc
      841 ttccacgaaa tgtagcatga cgcgatacgg ctattcatca cgataccgat gtgattccag
      901 acacgactat caatgcgaca tcagcactgg aaccgtcatg tgctgaaaca gctcgcggct
      961 gacgccgccg gtcaagatct cgcggagtgg cgaatgatga taagccccgg caacgatcag
     1021 gtcggcggag aggtcagcgg cactcgatag cagcacgtcg gagatggcaa tgttggtccg
     1081 tactgtctcg accgcgtggg cggcgattcc atgccgtgcc agatgaccaa cgatgcgtgc
     1141 catcggcagg cggtcgcgct cgaaatcgcg ctcccgggag tgcacggcca tcaccgtcac
     1201 gatttccgca gccccgatca gcggcagcgc atcattgagg gcccgcacgg cctcgcgaga
     1261 tccgtcccag gcgatcaaaa cacgacggcc aattgcggca aagctgccga tataggggat
     1321 catcagcacg ggacgaccac aggcgagaac aatctcctcc ggccgccatg ccggccccag
     1381 ccgggcatgg ggattcactt gaccgatgat gatcagatcg gcggcctgcg cgagggtgat
     1441 taggcctgcc gtatcgctcc gctccatcgg ataccagtcg ccttgagcgt gcaaggcgcg
     1501 aacggcgtcc cggaattgtt gttcggcagt ttcggcgagc atggcacctg gtgagatatc
     1561 ggtcgcactg acgatcaatg ggcttgccgc tgacccaaat cctgtaccac cttggttcga
     1621 cggcgagcca gctgccggat cgagcagaaa ggcggcgctg agatacgcct ggtggtcccg
     1681 agccatctgc atcgccagcc ggagacgttc ctcgccggca gcgccggcat cgagaaagac
     1741 gaggatatct ttcaaattca tcagcccctc ccacgcttgg cattgttgtt ttcgcgatcg
     1801 ctcaccgtgc tcaaggcaaa accctggtct cacactgcat catttggcaa gtcgctgcgt
     1861 ggccggacgg gaggaattcg tggcgagcca ccggggctcc ggacaacggc tgcgccgcgg
     1921 acttgccggg ctgctgcatg acgatgcgac gacgctggcg ttctttgacg gccttatttt
     1981 gtcagatgac gaaaaataga tgtgctgagg ctgggtggat accgcgacgc ttgatctccc
     2041 atggg
//
LOCUS       DASWQN010000974         3220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1676844, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000974 DASWQN010000000
VERSION     DASWQN010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676844"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VGC82_16660"
     CDS             <1..1164
                     /locus_tag="VGC82_16660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0424952.1"
                     /translation="LAALGDIIGYRRVYLWGLGVFTAASLACGLVETLPLLIAGRVLQ
                     GFGAAGIMSVNTALVRFIFPRTQLGRGISIIALVVAACSAAGPSVAAAILSVASWHWL
                     FTFNVPFGVLAVWLALRSLPETPRSRHRFDIISAALNAATFGLLLVGLDGIGHGQSGV
                     WVALELGVGTLACVMFIRRQNRMAVPMLPVDLFRKPIFALSVATAVCSHSAQTVAFLA
                     LPFYFAVAGGMSQSQIGLLITPWPAVVVVVAPISGRLADKYPAGLLGGLGLAVLTVGL
                     LLMLALPPNPPFLDVVWRMMVCGIGFGFFQSPNNRALISAAPRSRSGVAGGVLSTARL
                     TGQTIGGVVVAIIFALTHGDIGGGVGAALTAGAVLSGFACVISFLRLTSSDRL"
     gene            1278..1517
                     /locus_tag="VGC82_16665"
     CDS             1278..1517
                     /locus_tag="VGC82_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEY0424953.1"
                     /translation="MPLYIKDDSTARLVAEFARLRGITKQDAVKVAVQAELDRAEQAV
                     PLRVRFAALRRASPLPPATGDVADKAFFDELSGGL"
     gene            1514..1942
                     /locus_tag="VGC82_16670"
     CDS             1514..1942
                     /locus_tag="VGC82_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0424954.1"
                     /translation="MTIFADASALIAIIAGEDDADTMADALESDQTRLCSALSVWETM
                     AGLRRSYALSVSAARARIQLFLEVGGLHFVTMGEQEWIIAAEAYARFGKGRHPAALNM
                     GDCFAYACAKSNRARLLYKGDDFRQTDLAWDPESIKPPGF"
     gene            complement(1997..2797)
                     /locus_tag="VGC82_16675"
     CDS             complement(1997..2797)
                     /locus_tag="VGC82_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424955.1"
                     /translation="MSLPPYLVSAVNPSKASENKIHDDATARRFGFKGGFVGGVNVYA
                     YMSHQPVRRWGRAWLERGTGEAKFAKPVYDGDVAEVVAVEDADGIALTVHSQGVLCAT
                     GRAGLPEDPGVVPVLGDFKAVAPRDNQAPANEASLRVGDWLGMIPLPITEASHAQDLR
                     DIGETDPVYARERIVHPITIMRCCNWALSHNVTLPAWIHMGSTVRNLGAAHVGDTLNV
                     RARITKNYEHKGHKWVELDAMVVANETTPILRATHIAIYRPRQLAEAA"
     gene            complement(2885..>3220)
                     /locus_tag="VGC82_16680"
     CDS             complement(2885..>3220)
                     /locus_tag="VGC82_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424956.1"
                     /translation="NTSAESRLIYRETTDPIPEYDAHHIQITLADFSGPHKKLLERGL
                     ITEESDQHQYRFLDIVDVDTNKPLFRIEHETRSMRHPMFGRTFINRNPDMNNRNFVPG
                     YEVGLWALN"
BASE COUNT          506 a          981 c         1036 g          697 t
ORIGIN      
        1 ctggcggcgc tgggcgacat catcggctac cgccgggtct atctgtgggg gctcggggtc
       61 ttcaccgctg cgtcgttggc ctgcggattg gtcgagacgc tgcctctgct gatcgccggg
      121 cgcgtcctgc aaggcttcgg cgccgccggc atcatgagcg tcaacaccgc cctggtgcgg
      181 ttcatcttcc cccgaaccca gctcggccga gggatctcga tcatagcgtt ggtcgtggca
      241 gcctgttccg ccgccggccc atcggtggcg gcggcgatcc tgtcggttgc ttcgtggcat
      301 tggctgttca cgttcaacgt gccattcggc gtgctggcgg tatggttggc gttgcggtca
      361 ctgccggaga cgccgagatc ccggcaccgt ttcgacatca tcagcgcggc gctgaatgcg
      421 gcgacttttg gcctgctgct ggtcggcttg gacggaatcg gccacggcca gagcggcgtc
      481 tgggtcgcgc tggagctggg cgtcggcacg ttggcctgcg tcatgttcat ccggcggcag
      541 aaccgcatgg cagtgccgat gctgccggtc gatctgtttc gcaagccgat tttcgcgctg
      601 tcggtggcga cggcggtatg ctcccacagc gcccaaaccg tcgcgttcct ggcactgccg
      661 ttctacttcg cggttgccgg ggggatgtcg caatcgcaga tcggcttgct gatcacgccc
      721 tggccggcgg tcgtggtggt tgtggccccg atctcgggcc ggctggcgga caaatatccg
      781 gcagggctgc tcggcggatt gggtctggct gtgttgacgg tgggtctgtt gctgatgctg
      841 gcgctgccgc cgaacccacc attcctggac gtggtgtggc ggatgatggt gtgcgggatc
      901 ggctttgggt tcttccagtc gccgaataac cgggcgttga tctcggccgc gccacgatcg
      961 cgcagcggcg tggcgggtgg ggttctgtca accgcgagac tgaccgggca gacgattggt
     1021 ggcgttgtcg tggcgatcat attcgcattg acgcacggcg acattggtgg cggggtgggc
     1081 gcggcgctga cagccggggc ggtcctatcc gggtttgcct gcgtcatcag tttcctgcgc
     1141 ctgacgagct ccgacaggct ttgaaccatg gacggccttc ctgcgggagg cggtcgccga
     1201 gagccgcgcg ttggcccata atccagcttt gactttttgg atatccaatt ctagtatacg
     1261 gatattcgga gattatcatg cccctctata taaaggacga ttcaaccgct cggcttgtcg
     1321 ccgagtttgc caggctccgc gggatcacca agcaggatgc ggtaaaggtt gccgtacagg
     1381 cggaattgga ccgggccgaa caggccgtgc cgcttcgggt tcgattcgcg gcgttaagac
     1441 gcgcctctcc gcttccacct gccaccggtg acgttgccga taaggcattt ttcgacgaac
     1501 tgtcgggcgg tctgtgacga ttttcgccga cgcatcagct cttattgcca tcatcgctgg
     1561 ggaagatgat gccgatacaa tggccgatgc gctggagtcc gatcagactc gtctttgttc
     1621 agccttgtcg gtttgggaaa caatggcggg tcttcgcaga tcgtacgcgc tgtcggtttc
     1681 agccgcacgc gcgcggattc agttgttcct ggaggttggc ggcctccact tcgtcacgat
     1741 gggtgagcag gaatggatca tagcggccga ggcctatgcc cggttcggca agggacgcca
     1801 tcccgcggca ctgaacatgg gcgattgctt cgcgtatgcc tgcgccaaat ccaatcgggc
     1861 gagactgctc tataagggcg acgactttcg acagacggat cttgcttggg atccggagag
     1921 tatcaaaccc ccaggcttct gattcgggcc agtcgagcag cggtcatctc tgtagcggct
     1981 tgtatcatgc ccgtcattac gccgcctcgg ccaactgccg cggccggtag atcgcgatgt
     2041 gcgtcgcccg caggatcggg gtcgtctcgt tcgcaaccac catcgcgtcg agctccaccc
     2101 atttgtggcc cttgtgctcg tagttcttcg tgatcctcgc gcgcacgttc agcgtatcgc
     2161 cgacatgggc ggcgcccagg ttccgcaccg tgctgcccat gtgaatccat gccggcaatg
     2221 tgacgttgtg actcagcgcc cagttgcagc aacgcatgat cgttatcggg tgaacgatac
     2281 gttcccgtgc ataaaccgga tcggtttcgc cgatatcccg cagatcctgc gcgtgcgagg
     2341 cttcggtgat cggcagcgga atcatcccaa gccagtcgcc taccctcaat gacgcctcat
     2401 tcgcgggcgc ttggttgtcg cggggcgcca ccgccttgaa gtcccccagc accggcacca
     2461 ccccgggatc ctccggcaac ccggctcggc cagtggcgca cagcacgccc tggctgtgca
     2521 ccgtcagcgc tatcccgtcc gcgtcctcaa cggcgacgac ttcggcgacg tcgccgtcat
     2581 aaaccggctt cgcgaacttc gcttcgccgg ttccgcgctc cagccacgcc cgtccccagc
     2641 gccgcaccgg ttggtgcgac atgtaggcat acacgttcac gccgccgacg aagccaccct
     2701 tgaacccaaa acgccgagcg gtggcatcgt catggatctt gttctccgac gccttggacg
     2761 ggttgacggc agagacgaga taaggcggta atgacatcga aaagtccctg ctcttgttga
     2821 ccgactgact ccggcaaggc ttggcatggc aggtcttcac ctgccatgcc cgcgaagcag
     2881 aacgtcagtt cagcgcccac aaaccaacct catagcccgg aacgaagttg cggttgttca
     2941 tatccgggtt ccggttgatg aaggtgcggc cgaacatcgg atgccgcatg ctgcgggttt
     3001 cgtgctcgat acggaacagc ggcttgttcg tatcgacatc gacgatatcc aggaagcgat
     3061 actggtgttg gtcgctctcc tcggtgatca gcccgcgctc cagcagtttc ttgtgcgggc
     3121 cggagaagtc ggcgagcgtg atctggatgt ggtgggcgtc atattccgga atagggtcgg
     3181 tcgtttcgcg atagatcagc ctgctctcgg ccgaggtgtt
//
LOCUS       DASWQN010000975         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1940907, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000975 DASWQN010000000
VERSION     DASWQN010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940907"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VGC82_16685"
     CDS             <1..450
                     /locus_tag="VGC82_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY0424957.1"
                     /translation="IPGGASVPLIPKSVCDDMLMDFDSLRAAGTGLGTAAVIVMDKST
                     DVIKAIARLSKFYMHESCGQCTPCREGTGWMWRVMTRMVEGRATPDEIDTLQQVTKQI
                     EGHTICALGDAAAWPIQGLIRHFRPVMEERIAAYKSRGARPVPMAAE"
     gene            455..>2210
                     /gene="nuoG"
                     /locus_tag="VGC82_16690"
     CDS             455..>2210
                     /gene="nuoG"
                     /locus_tag="VGC82_16690"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEY0424958.1"
                     /translation="MPKVTIDGITVEAAAGSTILQAAEQAGVEIPRFCYHERLSIAGN
                     CRMCLVEVEKAPPKPIASCGYPVAEGMVVHTDTKMVRNARRGVMEFLLINHPLDCPIC
                     DQGGECDLQDQAMGYGMDHSRYAENKRAIPDKNLGPLVKTVMNRCIHCTRCIRFITEV
                     AGVPDLGATNRGEHMEVTTYVEKALTSELSGNIIDLCPVGALTSKPYAFIARPWELAK
                     VDSIDVLDAIGTNIRVDARGPEVLRILPRVNEDVNEEWIGDKSRFCIDGLKRRRLDRP
                     WIRENGKLRAATWNEAFDAIAARLKPLPGDRVGAIAGDLCDAESIIALKDLLASLGSA
                     NLDCRQDGARLDASRRDFYCFNTSIAGIEEADAILIVGSNPRKEAPVLNARIRKAWLA
                     NGMPVGVIGTATDLTYAVTALGSSPSVLTDLHAGSHDFAKVLANAKKPMIIVGQGALA
                     RQDGTAILAATWRLAAQVGALSQDWHGFNVLHTAASRVGALDLGFVPGPNGKAVDAML
                     GGGVDLLWLLGADGFDPSRIAANTFVVYQGHHGDAGARRADVILPGAAYTEKSGTYVN
                     TEGRVQRAFRAVYPPGEAR"
BASE COUNT          406 a          696 c          743 g          365 t
ORIGIN      
        1 attccgggtg gcgcttccgt gcccctgatc ccgaagtcgg tctgcgacga tatgctgatg
       61 gatttcgaca gtctgcgtgc ggcaggcacg ggactaggca ccgccgccgt catcgtgatg
      121 gataaatcca ccgacgtaat caaggcgatt gctcggttgt ccaagttcta catgcacgaa
      181 agctgcggcc agtgcacgcc gtgccgcgag gggactggct ggatgtggcg cgtcatgacc
      241 cgcatggtgg aaggccgcgc gacccccgat gagatcgata cgctgcagca agtgaccaag
      301 cagatcgaag gtcatacgat ctgtgcgttg ggcgatgcgg cggcctggcc gatccaggga
      361 ctgatcaggc atttccgtcc ggtcatggaa gaacggatcg cagcttacaa gtcacgcggc
      421 gcaaggccgg tgccgatggc ggcggagtag caccatgccc aaggtcacca tcgacggaat
      481 cacggtcgaa gccgccgccg gctccaccat cctgcaggca gccgaacagg cgggcgtcga
      541 aattccgcgc ttctgctacc acgagcgcct gtccatcgcc ggcaactgcc ggatgtgcct
      601 ggtggaggta gagaaagctc cacccaagcc gatcgcctcc tgcggctatc cagttgccga
      661 gggcatggtc gtgcataccg acaccaagat ggtgcgcaac gcccgccgcg gcgtgatgga
      721 attcttgctg atcaaccacc cgctggattg cccgatctgc gaccagggcg gtgagtgcga
      781 cctgcaggac caggccatgg gctacggcat ggatcattcg cgctacgccg agaacaagcg
      841 cgcgataccg gacaagaacc tgggaccgct ggtcaagacg gtgatgaacc gctgcatcca
      901 ttgcacgcgg tgcatccggt tcattaccga agtggccggc gtgccggatc ttggcgccac
      961 caaccgcggc gagcacatgg aagtcacgac ctacgtcgaa aaggcgctga cgtcggaact
     1021 gtcgggcaat atcatcgacc tttgcccggt cggggcgctg acctcgaagc cctatgcgtt
     1081 catcgcccgc ccgtgggaac tggcaaaagt cgacagcatc gacgtgctgg acgcgatcgg
     1141 aaccaacatc cgcgtcgatg cccgcggccc cgaagtgctg cgcattctcc cgcgcgtcaa
     1201 tgaggacgtg aacgaggagt ggatcggcga taagtcgcgt ttctgcatcg acggcctgaa
     1261 gcgccgccgg ctggatcgtc catggatccg tgaaaacggc aagctgcgtg cggccacctg
     1321 gaacgaggcg ttcgacgcca tcgccgccag gctgaagccg ctgcccggcg accgcgtcgg
     1381 ggcgatcgcc ggcgacctgt gcgacgccga aagcatcatc gcgctgaagg atctgctggc
     1441 cagcctggga tcggcgaacc tcgattgccg gcaggacggc gcccggctgg acgcttcgcg
     1501 gcgggacttc tactgcttca atacgtcgat tgccggaatc gaggaagccg acgcgatcct
     1561 gatcgtggga tcgaacccgc gcaaggaagc gccggtgctg aacgcccgca tccgtaaagc
     1621 gtggctggcg aacggaatgc ccgttggcgt gatcggcacc gcgacggatt tgacctacgc
     1681 ggtgaccgcg ctggggtcct cgccgtctgt gttgaccgat ctgcatgccg gctcgcacga
     1741 cttcgccaag gtactggcga atgcgaagaa gccgatgatc attgtcggcc agggcgcgct
     1801 cgcgcggcag gatggcacgg cgatactggc ggcgacctgg cggctcgcgg cccaggtcgg
     1861 cgcgctgtcg caggattggc atggtttcaa cgtgctgcac accgctgcga gccgggtcgg
     1921 tgcgctggac ctcggctttg tccctggccc aaacggcaag gccgtggacg cgatgctggg
     1981 cggcggcgtc gatctgctct ggctgctcgg ggccgacgga ttcgacccct cccgcatcgc
     2041 ggcgaacacg tttgtggttt accagggcca ccacggcgac gccggcgcgc gacgggcgga
     2101 cgtgatcctg ccgggcgccg cctatacgga aaaatccggc acgtatgtga acaccgaggg
     2161 ccgtgtccag cgcgccttca gggccgtcta tccgccgggt gaggcgcggg
//
LOCUS       DASWQN010000976         3323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_489080, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000976 DASWQN010000000
VERSION     DASWQN010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489080"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..199)
                     /locus_tag="VGC82_16695"
     CDS             complement(17..199)
                     /locus_tag="VGC82_16695"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY0424959.1"
                     /translation="MDKDRIEGAAHEAKGAVKQVIGKVTGDAKTEAEGAAEKAAGKVQ
                     NAVGGVKDSAREALDK"
     gene            complement(232..393)
                     /locus_tag="VGC82_16700"
     CDS             complement(232..393)
                     /locus_tag="VGC82_16700"
                     /inference="COORDINATES: protein motif:HMM:NF019696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEY0424960.1"
                     /translation="MSKNPSLLLAFAVLIAATPMLSACYTARGAGQDLSAAGRSIEKS
                     ADRNTGYKP"
     gene            595..1053
                     /locus_tag="VGC82_16705"
     CDS             595..1053
                     /locus_tag="VGC82_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY0424961.1"
                     /translation="MAGLYYEQFSVGQAFDHPWTRTVTEMDNTLFSALTMNVQPLHLD
                     AHFAAETEFGQRIVNSIFTLGLMVGISVNDTTIGTTVGNLGFTEMKFPKPVFHGDTLR
                     ARTKVLTMRESRSRPDVGIVEFEHTCWNQRDEMVAICRRQAMMRKLPKPA"
     gene            complement(1618..3024)
                     /locus_tag="VGC82_16710"
     CDS             complement(1618..3024)
                     /locus_tag="VGC82_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY0424962.1"
                     /translation="MSASLTSLAYLVAAVLFILALRGLSSPVTSREGNRFGMIGMAVA
                     VVATLLQHGMGSWGYLLIVLGICIGGGAGIVVAQRIQMTALPQLVAAFHSLVGLAAVF
                     VAAAALNAPEAFNIGIPGDIHTESLVEMSLGLAIGAITFSGSLIAFAKLQALMKGAPI
                     TFPNQHWLNLGLAILLFVLIVGFVASGGHQLLFWLIALLSLTLGFLIIIPIGGADMPV
                     VVSMLNSYSGWAAAGIGFTIQNLALIITGSLVGASGAILSYIMCKGMNRSIINVLLGG
                     FGSETGGAAAAGVTGDQTVRSGNAEDAAFIMKNASKVIVVPGYGMAVAQAQHALREMT
                     DTLKREGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEEINNEFSTADVVYVI
                     GANDVTNPSAKTDPSSPIYGMPILDVEKARTVLFVKRSMASGYAGVDNVLFFRPNTMM
                     LFGDAKKMTEEIVQALGH"
     gene            complement(3035..>3323)
                     /locus_tag="VGC82_16715"
     CDS             complement(3035..>3323)
                     /locus_tag="VGC82_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HEY0424963.1"
                     /translation="PEVNPFIFLLAVFLMACFVGYYVVWNVTPALHSPLMAVTNAISS
                     VIIVGAMLATGLATSGWAMLFGFIAVTLASVNIFGGFMVTQRMLSMFRKKQ"
BASE COUNT          673 a         1012 c         1042 g          596 t
ORIGIN      
        1 aacaactatg cgttggctat ttatcgagcg cctcgcgggc agaatcctta acgccgccta
       61 cggcgttctg gaccttgccg gcagcctttt cggcggcacc ctcggcttcg gttttcgcgt
      121 cgccggttac tttgccgatg acttgcttga cggcgccttt ggcctcatga gcggcgcctt
      181 cgatacggtc tttatccatg atcaaagtcc tttgattgtc attgcggatt atcagggttt
      241 gtagccggtg ttgcggtcgg cggatttctc gatgctcctg ccggccgcgc taagatcctg
      301 gccggcgccg cgcgccgtat agcaagccga gagcatcggc gtcgccgcaa tgagtacggc
      361 gaaggccagc agcagactcg ggtttttgct catgtcgtag tcctttccgg caagcttggc
      421 gtcccgtaac gttgcgttcc cgccgagtgg ttgcaagttg taaagagttt gcatccgatc
      481 atgttgacct cacatgacct tgaggtggcg tgatgtcggc ggcgttccgt cccttggcat
      541 ccagcggcga atgcggttga ctcccggcct cggtcaacgg gaaggcggca cgagatggcg
      601 gggctctatt acgaacagtt ttcggtcggc caagcgttcg atcatccctg gacccgaacg
      661 gtgaccgaga tggacaacac gctgttcagc gcgctgacga tgaatgtcca gccgcttcat
      721 ctcgacgcgc atttcgcggc cgaaaccgag ttcggtcagc gcattgtgaa ctcgatcttc
      781 acgctcggtc tgatggtggg tataagcgtg aacgacacga cgatcggcac cacggtcggc
      841 aatctgggtt ttaccgagat gaagttcccg aagccggtgt tccacggcga tacgctgcgc
      901 gcccggacga aggtgctgac gatgcgggaa agccggtcgc gccccgatgt cggtatcgtc
      961 gagttcgagc acacgtgttg gaatcagcgc gatgagatgg tggcgatctg ccggcggcag
     1021 gcgatgatgc gcaagctgcc gaagccggca tgaacgaggg gcaatcgcgg gacaagccgg
     1081 ctaaaccgcc ggtcaacccg gatcgcacgg cacgccaagc cgcggcgttg cgggcgaacc
     1141 tgggcaagcg caaggatcag gcgcgacggc gtgaatcaga gaaggaaaag ccccagcctg
     1201 aaggcggaaa cgacctagtg tcctgccccg gaaggtcatc gcatcatgcg gcggacgcag
     1261 gggatcagca ggccactgaa aacaaagaga gagtggcgcg agactgaggt tcgcaggata
     1321 tggcggactt cagattcggg acaccagccg cgggcatggg ccccttggct agggtctgtc
     1381 ggtaactaac cgcttcaagc gtgatctcaa atccggcatg gtgaacgttg gacgatcgtg
     1441 tcatgcccgt gggcgtggcg cggccacccc cgactttgtc tcaacagcgg aagtcgtgga
     1501 taaccggcac gcggccgatc atgacacaat cgccgaagac cgcgctgtta gagtccgacg
     1561 ctcaaggtgt gatgccgacc atgacgttgt ccggggagga tcaagcggct caagccctca
     1621 gtgacccagc gcctggacga tctcctcggt catcttcttt gcgtcgccga atagcatcat
     1681 ggtattcggc cggaagaaca atacgttatc gacgccggca tagcccgacg ccatcgagcg
     1741 cttcacgaac agcaccgtac gagccttctc cacgtccagg atgggcatgc catagatcgg
     1801 cgatgacgga tcggtcttcg ccgatggatt ggtgacgtcg ttggcgccga tgacgtacac
     1861 cacatcggcc gtagagaact cgttattgat ttcttcgagt tcgaacacct cgtcatacgg
     1921 cacattggct tcggccagca gcacgttcat gtgcccgggc atgcggccgg ccaccggatg
     1981 gatggcgtat ttcacctcca ccccttccct tttcagcgtg tccgtcatct cccgcagtgc
     2041 atgctgtgcc tgggcgacgg ccatgccgta tcccggcacg acgatgacct tcgaggcgtt
     2101 tttcatgatg aaggcggcat cctcggcgtt gcccgaccgg accgtctggt cgccggtgac
     2161 cccggccgcc gccgcgccac cggtttcgga accgaagccg cccagcagca cattgatgat
     2221 gctgcggttc atgcccttgc acatgatgta ggacaggatc gcgcccgacg cgccgaccag
     2281 cgaaccggtg atgatcaggg ccaggttctg aatggtgaag ccgatgcccg ccgccgccca
     2341 gccggagtag ctgttcagca tcgacaccac caccggcatg tcggcgccgc caatcgggat
     2401 gatgatcagg aagcccagcg tcagcgacag cagagcgatc aaccagaaca gcaactggtg
     2461 gccgcccgag gcgacgaacc cgacgatcag cacgaacagc aggatcgcca gccccaggtt
     2521 cagccagtgc tggttgggga aggtaatcgg cgcgcccttc atcagcgcct gcaacttggc
     2581 gaacgcgatc aggctgccgg agaaggtgat ggcaccgatc gccagtccca gcgacatctc
     2641 gaccaggctt tcggtgtgga tgtcaccggg gatgccgatg ttgaaggcct cgggggcgtt
     2701 cagcgccgcc gccgcgacga acaccgccgc caaccccacc agcgagtgga acgccgcgac
     2761 caactgcggc agcgcggtca tctggatgcg ctgcgcaacc acgatgccgg cgccaccgcc
     2821 gatgcagatg ccgagcacga tgaggagata gccccaggag cccatgccgt gctgcagcag
     2881 cgttgcgaca acggcgaccg ccatgccgat catgccgaag cggttgccct cccgcgaggt
     2941 aacgggcgat gacaggccac gcagcgcaag gatgaacagg acggcggcga ccagataagc
     3001 gagtgacgtc aagctggcgg acattggtgc ggccttattg cttcttccgg aacatcgaaa
     3061 gcattcgctg cgtcaccatg aagccgccga agatattgac cgaggcgagg gtgacggcga
     3121 tgaacccgaa cagcatcgcc caccccgacg tggccaatcc ggttgccagc atcgcgccaa
     3181 cgatgatcac cgaggaaatc gcattcgtca ccgccatcag cggcgagtgc agcgccgggg
     3241 tcacgttcca gacgacataa tagccgacga agcaggccat caggaaaacc gccagcagga
     3301 agatgaatgg attgacctcg ggt
//
LOCUS       DASWQN010000977         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1414127, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000977 DASWQN010000000
VERSION     DASWQN010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414127"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2716
                     /locus_tag="VGC82_16720"
     CDS             <1..2716
                     /locus_tag="VGC82_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0424964.1"
                     /translation="DSALNFIRPALAPVPGAALPLPFGGKVRQVQADLNQQALHQYGV
                     SANDVINALSLQNLITPVGTQKIGTYEYTVNLNDSPKAVAAFNDLPVKTVNGTVIYMR
                     DVAYVHDGSPPQTNAVHVNGASAVLLTIMKAGTSSTLDIINGVKNLLPSVSQTLPGSL
                     KLTAVGDQSVFVTDAVSSVVKEGVIAAVLTGMMILLFLGSWRSTLIITLSIPLAILAA
                     LTGLSLLGETINVMTLGGLALAVGILVDDATVTIENINWHLEQGKDIEPAIMDGARQI
                     VVPATVSLLCICIAFVPMFGLGGVAGYLFRPMAEAVMLALAASYVLSRTLVPTLANYL
                     LRHQHARGASGDAAAAPVKASRNPLVRFQHGFERLFTKVRDGYLGLLQLCLQHRIKLI
                     AGFLGLTLLSFGLAPFLGQDFFPTVDGGQIKLHIRAPTGTRIEETTSLTDRIGTAIHG
                     IIPASELDGVVSNIGLTVSGINMAYNNSGTIGVGDADILISLKPNHAPTDDYIKTMRV
                     KLPQQFPGTSFAFLPADIVSQVLNFGVPAPIDLQVAGRDLAADRTYANSLLAKIREIP
                     GIADARIQQAFQQPTLDVNVDRSLTSLVGLTEKDVATAMLTTLSGSSQSAPTYWLNPA
                     NGVSYAVSVQTPQRDINTMTGLKNIPVSSATANPQLLGGLAQVQRTNSNAVVSHYNVQ
                     PVIDIFATPQGRDLGALAADIQQVVHATAKDLPKGASVALRGQVSTMTSAYQQLYVGL
                     AAAIVLIYLLIVINFQSWIDPFVIVMALPTALAGIVWMLFATGTTLSVPALTGAIMCM
                     GVATANSILVISFARERMEAGLDALAAAFEAGRSRFRPVLMTALAMVIGMLPMAIEPG
                     QNAPLGRAVIGGLIFATCATLFLVPTIFGLVHGRQRPAAANAATTSSD"
     gene            2729..>3309
                     /locus_tag="VGC82_16725"
     CDS             2729..>3309
                     /locus_tag="VGC82_16725"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY0424965.1"
                     /translation="MASQDIKAPTRRSLLTTAAAAALVAAVVVGYGFISRVQSKQEVV
                     DWTQAQSVPTVALAQLTPAGANQTLTLPGNVQPLNRAAIYARVNGYVKSWDHDIGSPV
                     KAGQVLATIDAPDLDQQLSQARATLASVKANEEIATLTANRNNILLQKQIVAQQLADQ
                     TTADAAAKKAVVDANEANVKQLEAMQSFKTLASP"
BASE COUNT          593 a         1093 c         1027 g          596 t
ORIGIN      
        1 cgattccgcg cttaatttca tccgccctgc gctggcgccc gttcccggcg ccgcgctgcc
       61 gcttccgttc ggcggtaagg tgcgccaggt ccaggctgat cttaaccagc aggcgctgca
      121 tcaatacggt gtttccgcca acgacgtcat caacgcgctt tcgctgcaaa acctgatcac
      181 gccggttggg acccaaaaga tcggcacgta cgaatatacc gtcaatttga acgactcgcc
      241 gaaagcggtc gcagccttca acgacctccc ggtcaagacc gtcaacggca ccgtcatcta
      301 tatgcgtgac gtggcctatg tgcatgacgg cagtccgccg cagaccaacg cggttcacgt
      361 caacggcgcc agcgccgtgc tgctgaccat catgaaggcg ggcacgagct cgacgctcga
      421 catcatcaac ggcgtcaaga acctgctgcc ctccgtctcg cagacgcttc cgggcagcct
      481 gaagctgacc gcggtgggcg accagtcggt tttcgtcacc gacgcggtgt ccagcgtggt
      541 caaggagggc gtgatcgcag cggtgctgac cgggatgatg attttgctgt tccttggcag
      601 ctggcgctcg accctcatca tcacgctttc gatcccgctg gcgattttgg cggcgctgac
      661 cggtctatcg ctgctgggcg agaccatcaa cgtcatgacg ctcggcggcc tggcgcttgc
      721 ggtcggtatt ctggtcgacg acgccaccgt caccatcgag aacatcaact ggcatctcga
      781 gcaaggtaag gacatcgagc cagcgatcat ggacggcgcg cggcagatcg tggtgccggc
      841 gaccgtgtcg ctgctctgca tctgcatcgc tttcgtgccg atgttcggcc tcggcggcgt
      901 cgcgggttat ctttttcggc cgatggccga ggcggtgatg ctggcgctcg ccgcgtccta
      961 tgtgctgtcg cggaccctgg ttccgaccct ggccaactat ctgctgcgcc atcagcacgc
     1021 gcgcggcgcg tcgggggacg cggctgccgc cccggtgaaa gccagccgca atccgctcgt
     1081 gcggttccag cacggcttcg aacgtttgtt caccaaggtc cgcgacggct atctgggcct
     1141 gctgcagctc tgcctgcagc accggatcaa gctgatcgcc ggattcctcg ggttgaccct
     1201 gctgtcgttc gggcttgcgc cgtttctcgg ccaggacttt tttccaaccg tggatggcgg
     1261 ccagatcaag cttcatattc gtgcgccgac gggtacgcgg atcgaggaga cgaccagcct
     1321 gaccgaccgg atcggcaccg ccatccacgg catcattccg gccagcgaac tcgatggcgt
     1381 cgtgagcaac atcgggctca cggtcagcgg catcaacatg gcgtacaata attccggcac
     1441 catcggggtc ggagacgccg acatcctgat cagcctcaag cccaatcacg cgccgaccga
     1501 tgactacatc aagacgatgc gggtcaagct gccgcagcag ttccccggca ccagcttcgc
     1561 gttcctgcct gcggacatcg tcagccaggt attgaatttc ggcgtgcccg cgccgatcga
     1621 cctgcaggtt gcgggcaggg accttgccgc ggatcggacc tatgcgaatt cgctgctggc
     1681 caaaatccgg gaaatcccgg gtattgccga cgcgcgcatc cagcaggcgt ttcagcagcc
     1741 gacgctcgac gtcaacgtcg atcgctcgct gacctcgctg gtcggcctga ccgagaagga
     1801 cgtcgccacc gcgatgctga cgacgctgtc aggaagctcg cagtcggcgc cgacctattg
     1861 gctgaatccc gccaacggcg tgtcttacgc ggtctcggtc cagaccccgc agcgcgacat
     1921 caacaccatg accggcttga aaaacatccc ggtgtcgtcc gctaccgcca atccccagct
     1981 gctcggcggc ctggcgcagg tccagcggac caacagcaac gcggtggtgt cgcactataa
     2041 cgtgcagccc gtgatcgaca ttttcgcgac gccccagggg cgcgatctcg gtgcgcttgc
     2101 cgccgacatt cagcaggtgg tgcatgccac cgccaaagac ctgcccaagg gcgcgagcgt
     2161 ggcgctgcgc gggcaggtaa gcacgatgac cagcgcctat cagcagctct atgtcggcct
     2221 ggcggcggca atcgtgctga tctacctcct gatcgttatc aatttccaat cctggatcga
     2281 tccgttcgtc atcgtgatgg cgctcccgac ggcgttggcg ggtatcgtct ggatgctatt
     2341 cgcaacgggg acgacgcttt cggtgcccgc gctcaccggt gccatcatgt gcatgggcgt
     2401 ggcgacggcc aacagcatcc tggtgatcag ctttgcccgc gagcgcatgg aagccggcct
     2461 ggatgcgttg gcggcggcat tcgaggcggg ccgatcgcga tttcgtccag tgctgatgac
     2521 ggcgctcgcg atggtcatcg gcatgctgcc gatggcgatc gagcccggcc agaacgcgcc
     2581 attggggcgc gccgtgatcg gcgggctgat cttcgccacc tgcgccacct tgtttctggt
     2641 gccgacgatc ttcggtctgg tgcacggccg gcagcgcccc gccgcggcaa atgccgcaac
     2701 cacatcgtca gattaagggg agaacaaaat ggcctcgcaa gacatcaagg ctccgacccg
     2761 gcgcagcctg cttacgaccg ccgcggccgc ggcactcgtt gcggcggtcg tggtcggcta
     2821 tggtttcatc agccgggtcc agagcaaaca agaagtggtg gactggacgc aggcccagtc
     2881 cgtgccgacc gtcgcgctgg cccagctcac tcccgccggc gcgaaccaga cgctgacgct
     2941 gcccggaaat gtacagccgc tcaacagggc cgcgatctat gcgcgcgtga acggctatgt
     3001 gaagagctgg gaccacgaca tcggatcgcc ggtgaaggcc ggacaggtgc tggccaccat
     3061 cgacgcgccc gatctcgatc agcagctcag ccaggccagg gcgacgcttg cgagcgtcaa
     3121 ggccaacgag gagatcgcca cgctgactgc gaaccggaac aatatcttgc tgcagaagca
     3181 gatcgtcgcg cagcagctcg cggaccagac cactgccgat gccgcggcga agaaggcggt
     3241 ggtcgacgcc aacgaagcca acgtgaagca gctcgaggcc atgcaatcgt tcaagacgct
     3301 cgcctcgcc
//
LOCUS       DASWQN010000978         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2205535, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000978 DASWQN010000000
VERSION     DASWQN010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2205535"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VGC82_16730"
     CDS             <1..342
                     /locus_tag="VGC82_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0424966.1"
                     /translation="GVQGMRDRIRRELGNRLPTPRDFINITRRGTFHDHPRFVGSPKD
                     VADGLEEWFSTRACDGFVIAASHVPGAYEDFSRLVVPELQRRGLFHTDYTGTTLRENL
                     GLDRPAVGAWR"
     gene            360..974
                     /locus_tag="VGC82_16735"
     CDS             360..974
                     /locus_tag="VGC82_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0424967.1"
                     /translation="MKLYYAPGACSIGIHVLLEEIGKPYETERVNLQQGEQYKPPFTA
                     VNPKSKVPTLIRDDGTVMTEYPAIAYWLAKTNPFANLLPDDIDQQAKSLELTDYAVAT
                     IHMQGFARQFRSSNFTPNPADEEAVKSRGKEIAEKGFALIDKALEGKEYAVGKFSIAD
                     SAIFYTEFWSKRVGMSLPANCASHLERMLARPAVQRVMQQEGLA"
     gene            978..1556
                     /locus_tag="VGC82_16740"
     CDS             978..1556
                     /locus_tag="VGC82_16740"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0424968.1"
                     /translation="MSVSPADRLHPIAFWFLRHGETDWNAQNLSQGNVDIPLNETGLA
                     QARSASLLLRGRGIRNIISSPLSRAKVTADIAAAQLNLPVQIDDGLREVAFGVQEGKP
                     MSEWFQPWVDGLLTPEGAESFPALTRRAVAAINRCTARPPAVLVVAHGALFRAIRGAM
                     GHEPNVRTRNAVPIWCEPPKDGAKVWGITYAD"
     gene            complement(1721..>1930)
                     /locus_tag="VGC82_16745"
     CDS             complement(1721..>1930)
                     /locus_tag="VGC82_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424969.1"
                     /translation="EDQFNPNDHDLDEMTSIIPVKWPADRRRVGQRSVRFGLDRLSGP
                     PSEAPIGLSRHHARVSRGRRLLAAI"
BASE COUNT          378 a          593 c          613 g          346 t
ORIGIN      
        1 ggcgtgcagg gcatgcgcga ccgtattcgc cgtgagctcg gcaatcgcct gccgacaccg
       61 cgggacttca tcaatatcac ccggcgcggc acgtttcacg accacccgcg tttcgtcggc
      121 agcccgaagg acgtggccga cggcctggaa gaatggttct cgacacgcgc ctgcgacggc
      181 ttcgtcatcg ccgcgtcgca cgttccgggc gcttacgagg acttctcgcg gttggttgtc
      241 cctgaattgc aacgccgcgg cctgttccat acagactata ctgggactac gctgcgggag
      301 aacctgggtc tcgaccgtcc tgctgtcgga gcctggcgat aaaggaaagg agacacacca
      361 tgaaactcta ttacgcaccc ggcgcctgct cgatcggcat ccatgtgctg ctggaggaaa
      421 tcggcaagcc ctacgaaacc gaacgcgtca atttgcaaca aggcgagcaa tacaagccgc
      481 cgttcaccgc ggtcaatccg aaatcgaaag tgcccaccct gatccgcgac gacgggaccg
      541 tgatgacgga atatccggcg atcgcctatt ggctggctaa gaccaacccg ttcgccaatc
      601 tgctgccgga cgatatcgac cagcaggcca agtcgctgga gctgacggac tacgccgtag
      661 ccacgatcca catgcaaggc ttcgctcggc agttccgctc ctcgaacttc acgccgaacc
      721 ccgcggatga ggaggcggtg aagtcacggg gcaaggaaat cgccgaaaaa ggcttcgcgc
      781 tgatcgacaa ggcgctggag ggcaaggaat atgccgtggg caagttctcc atcgccgatt
      841 ccgcgatctt ctacacggag ttttggtcga agcgcgtcgg catgagcttg ccggccaact
      901 gtgcttctca tctggagcgc atgctggcac ggcccgccgt gcagcgtgtg atgcaacagg
      961 aaggcttggc ctgaaccatg agcgtatccc ctgccgacag gttgcatccc atcgcgttct
     1021 ggttcctgcg ccatggcgaa accgactgga acgcgcaaaa tctatcgcag gggaacgtcg
     1081 atatcccgct gaacgaaacc ggtctggcgc aggctcggtc cgcttcgctg ctgttgcggg
     1141 gacgcggtat ccgaaacatc atttcctcgc cgctgagccg cgccaaggtc accgcggata
     1201 tcgccgcggc ccaactgaac ctgccggtgc agatcgatga cggcctgcgg gaggtcgcat
     1261 tcggcgtgca ggaaggcaag ccaatgtcgg agtggtttca gccctgggtc gatgggctgc
     1321 tgacaccgga gggggcggaa agttttccgg cactgacgag gcgggcggtc gccgccatca
     1381 accgctgcac tgcccggccg cccgctgtgc tggtggtcgc gcacggggcg ctgtttcgcg
     1441 cgatacgcgg cgcgatgggc cacgagccaa atgtgcggac caggaacgcg gtgccgatct
     1501 ggtgcgaacc gccaaaggac ggcgcgaagg tctgggggat cacttacgcg gactgacatg
     1561 gcgaagtgac caacgctgcc catcccgggg acgcgttcta tctccggtga ttgcgttagc
     1621 cctgcctctc gcacggcgag gaaggcgccg ccgatggccg tttgtgcaaa ttggtctcgc
     1681 gatcctcggc tggctgttgg tcgccccggt gatcgccaga tcatatcgcg gccaataggc
     1741 ggcgaccgcg cgaaactcga gcgtggtgcc ttgataaacc aataggtgcc tcggaaggag
     1801 gaccggaaag ccggtccagg ccaaaccgga cagatcgctg gccgacacgt cggcgatcgg
     1861 ctggccattt taccgggatg atggaggtca tctcgtctag atcatgatcg ttgggattga
     1921 actgatcctc
//
LOCUS       DASWQN010000979         7297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1544541, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000979 DASWQN010000000
VERSION     DASWQN010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544541"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VGC82_16750"
     CDS             complement(<1..1238)
                     /locus_tag="VGC82_16750"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0424970.1"
                     /translation="MAYDLLIRNGRIIDGSGMPAFRGDLAVKDGKIVEIGKLSGQATR
                     VFDAGGMVVAPGFIDNHCHYDAQVSWDPMCSFSCDHGATTVIFGNCSLSLAPVRPGKS
                     ERLAEFLSYVEAIPMEVMRTVDFNWETVPQYIEQLDRNLGVNVGNLIGHTAVRYYVMG
                     DDCQKRAATDAEITAMQDIVRDGMKAGALGVSVSRNQGHYDPQGVHVPALWAEEKEIF
                     AYCDVLRELSTGIIQCGGGNGAEMKDGLMSRLSEATGRPVVYNNLNQSMRRPNEWKEQ
                     MAKVDASFAKGIRAYPTCTPNRITDFFTMRNTQVFRGLPTWHPLLLASHEEKLRAYND
                     PDIRRKLHQEAVEFKVDVMPPGICRTWWDYMVVQQAALAKNKDLEGKTVGEIAKAQGK
                     GIIDAFLDLVVEENLDTAFL"
     gene            1452..2684
                     /locus_tag="VGC82_16755"
     CDS             1452..2684
                     /locus_tag="VGC82_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HEY0424971.1"
                     /translation="MTDYAPLTLHVPEPAVRPGGTPDFSSVRIPEAGSVARPEVDADP
                     ESIRDLAYSIIRVLNRDGEAVGPWRGLLNDDELLEGLRHMMILRAFDTRMVMAQRQGK
                     TSFYMQHMGEEAVSCAVRKALLPGDMNFPTYRQAGLLIAAGHSLENMMCQIYSNERDP
                     LKGRQMPVLYSSKEDGFFSLSGNLATQYIQAVGWAMASAIKNDTKIAAAWIGDGATAE
                     SDFHAALVFASTYKAPVVLNIVNNQWAISTFQGIARGGSGTFAARGLGFGIPALRVDG
                     NDYLAVHAVAKWAAERARRNLGPTLIEHVTYRVGAHSTSDDPAVYRPKTESDAWPLGD
                     PVIRLKNHLIVRGVWSDDRHKQAEAEVLDTVITAQKEAESHGTLHAGGQPSVREMFED
                     VYAKMPPHLRRQRQQAGV"
     gene            2688..3701
                     /locus_tag="VGC82_16760"
     CDS             2688..3701
                     /locus_tag="VGC82_16760"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY0424972.1"
                     /translation="MSRKTMIEAIRDAMDVMMGRDENVVVFGEDVGFFGGVFRCTQGL
                     QAKYGKSRCFDAPINESGIVGAAVGMAAYGLRPCVEIQFADYMYPAYDQIVSEAARLR
                     YRSNGQFTCPMVVRMPTGGGISGGQTHSQSPEALFTHVAGLKTVVPSTPGDAKGLLIA
                     AIEDPDPVIFLEPKRLYNGPFDGHYDRPVTPWSKHELGEVPDGYYTLPLGKAAIRRPG
                     SAVTVLAYGTMVYVGLAAAAETGVDAEIIDLRTLVPLDLETIEASVRKTGRCVIIHEA
                     THTSGFGAELSALVQETCFYSIEKPIRRVTGWDTPYPHAQEWDYFPGPDRVGRALLET
                     MEP"
     gene            3703..5013
                     /locus_tag="VGC82_16765"
     CDS             3703..5013
                     /locus_tag="VGC82_16765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY0424973.1"
                     /translation="MAEQAIRLPDVGEGVAEAELVEWHVRIGEIIQEDAILAAVMTDK
                     ATVEIPSPIAGEVVWLAAEVGDVVAVGSDLVRVKIQGGGANPPAPEAKPLAASPAVLP
                     ARAPTGGSDTIAPVAPIQVTASAPDMPARNAGSRQLLVGAPRPEGERPLASPAVRRRA
                     QEAGMDLRQVPGTGPVGRITHQDLDAFFARGPGMAKAPGLQRRTDVEDVKIVGLRRRI
                     AEKMAIANTRIPHITYVEEVDVTALEQLRATLNSDERHDRPRLTLLPFLMRAIVRAVA
                     EQPGLNALFDDEAGVLHQHEGVHIGIAAQTAGGLLVPVVRHAEARDIWDCAVELNRLS
                     DAAKKATATRDELTGSTITITSLGAMGGVVTTPIINHPEVAIVGVNKMMMRPVWDGAT
                     FIPRKMMNLSSSFDHRVVDGYDAARFIQRLKTLLETPALIFLEG"
     gene            5018..6403
                     /gene="lpdA"
                     /locus_tag="VGC82_16770"
     CDS             5018..6403
                     /gene="lpdA"
                     /locus_tag="VGC82_16770"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY0424974.1"
                     /translation="MKDISCRLLIIGAGPGGYVCAIRAGQLGIDTVIVEAKKLGGTCL
                     NVGCIPSKALIHAADAFHGIAGSSSLGISAGKPELDLSKTMLWKDGIVGRLNNGVAAL
                     LKKAGVKVVEGWARFRDGKTVEVETEIGQQIIRAEQVVIATGSRAAELPFLPFGGNVI
                     SSTGALALSEVPNRLVVVGAGYIGLELGTAFAKLGASVTVVEAESRILPQYDAELTRP
                     VARRLRELGVEVLTGAKAKGFGRDRNALSTETADGEGRALPFDRVLVTVGRKPATEGW
                     DLDAIDLDMDGPFLRIDDKCRTSMRGVYAIGDVTGEPMLAHRAMAQGEMVAELVAGEN
                     RSWDKRCIPAVCFTDPEIVTAGLSPEQAEATGIKFVTGLFPFQANGRAMSLESEAGFI
                     RVVARADNHLLLGIQGVGSGIAELSAGFSLALEMAARLEDVAGTIHAHPTLGEGFLES
                     VHMALGSPIHI"
BASE COUNT         1372 a         2243 c         2314 g         1368 t
ORIGIN      
        1 tgcaggaacg cagtatcgag gttctcttcg acgaccaggt cgaggaacgc gtcgatgatg
       61 ccctttccct gtgctttcgc aatctcaccg acggtcttgc cctcaagatc cttattcttc
      121 gccagtgccg cttgttgcac caccatatag tcccaccatg tgcggcagat ccccggcggc
      181 atgacgtcaa ccttgaactc gacggcctcc tgatgaagct tccggcgtat atcggggtcg
      241 ttgtaggcgc gcagtttttc ctcatgagag gcgagtaaca gcggatgcca cgtcggcagc
      301 ccacggaaca cctgggtatt gcgcatcgtg aagaagtcgg tgatgcggtt gggtgtgcat
      361 gtcggatagg ctcggatgcc tttggcaaag gatgcgtcaa ccttcgccat ctgctccttc
      421 cactcgtttg gccgtcgcat cgactgattg aggttgttgt aaacgacagg gcggcctgtg
      481 gcctcggaca gacggctcat taacccgtct ttcatttcgg cgccattgcc gccgccgcat
      541 tggatgatgc cggtgctcag ttcgcgcaat acgtcgcagt acgcgaaaat ctctttctcc
      601 tcggcccata gtgccgggac gtgaacgcct tgcggatcgt aatggccttg attgcgtgac
      661 accgatacac cgagcgcacc ggctttcatg ccgtcccgca cgatgtcctg catcgccgtg
      721 atctcggcgt cggtcgctgc gcgtttttgg cagtcgtcgc ccatcacata gtagcggacg
      781 gcggtgtggc cgatcaagtt tccaacgttg accccaaggt tgcggtcaag ttgttcgatg
      841 tattgcggaa ccgtctccca gttgaaatcg acggttcgca tgacttccat tggaatcgct
      901 tcgacatagg acaggaactc cgccagccgc tccgactttc ccgggcgcac cggcgctagc
      961 gacagcgagc aattgccgaa aatcactgtg gtcgcgccgt ggtcgcatga aaacgagcac
     1021 atcgggtccc agcttacctg ggcatcgtaa tggcaatgat tatcgatgaa tccgggggcg
     1081 accaccatgc cgccggcatc gaagacccgc gtcgcttgcc cgctgagttt gccgatttca
     1141 acgattttgc cgtcctttac cgcgagatct ccgcggaacg ccggcattcc cgatccgtcg
     1201 ataatgcggc cattgcggat caacaaatcg tacgccatcg gattttctcc tttgtttagg
     1261 tcgccttatg ttgaacgtct cttcccgtcg caagtatggc acgcgcctgc ggtgacgcat
     1321 acccgcaacg cgacacctgg aacgtgacac ctgacaacgc gacgcctgac atcgcgacaa
     1381 cggggccgga accacccata tgcagacggc gaatggtccg catgggccgg atgcggacgg
     1441 gagacgaaga catgacagac tatgccccgc tgaccctgca tgtgccggaa ccggcggttc
     1501 gacccggcgg cacgccggat ttctccagcg tccgcattcc ggaggcggga tcggtggcac
     1561 gtccggaggt ggatgccgat cctgaatcga tccgcgacct ggcctattct atcatccgcg
     1621 ttctgaaccg cgacggcgag gcggttggcc cctggcgcgg cctgttgaac gacgacgaac
     1681 tgctcgaagg tcttcgccac atgatgatct tgcgcgcgtt cgacacccgg atggtcatgg
     1741 cgcagcggca gggtaaaacc tcgttctaca tgcagcacat gggcgaggag gcggtcagtt
     1801 gcgccgttcg caaggccctg ctgccgggcg acatgaattt tcccacgtat cgccaggcgg
     1861 gactgctgat cgccgccggg cactcgctgg aaaacatgat gtgccagatt tactccaacg
     1921 agcgagatcc cttgaagggc cggcagatgc cggtgctgta ttcgtcgaag gaggatgggt
     1981 ttttctcgct ttcggggaac ctggcgacgc agtacatcca ggcggtcggc tgggccatgg
     2041 cgtcggcgat caagaacgac accaagatcg cggcggcctg gattggcgat ggcgcgacgg
     2101 ccgagtccga tttccacgcc gcgctggtgt ttgcctccac ctacaaggcg ccggtggtgc
     2161 tgaacatcgt caacaatcaa tgggccattt ccaccttcca gggcattgcc cgcggcggct
     2221 ccggcacctt cgccgcccga ggccttggct tcggcatccc cgctctccgc gtggacggca
     2281 acgattatct tgcggtgcat gccgtggcga aatgggctgc cgaacgggcg cggcgcaacc
     2341 tcggcccgac gctgatcgag cacgttactt atcgcgtcgg tgctcattcc acctccgacg
     2401 acccggccgt ctatcgtccc aaaaccgagt ccgacgcctg gccgctcggt gatccggtga
     2461 tccggctgaa gaaccacctg atcgtccgcg gcgtctggtc ggatgaccgc cacaagcaag
     2521 ccgaggccga agtcctggac accgtgatca ccgcccagaa ggaggccgaa agtcacggca
     2581 cgttgcacgc cggcggccag ccctcggtgc gcgagatgtt cgaggatgtc tatgcaaaga
     2641 tgccgccgca tctgcgtcgt caacgccagc aggccggagt gtagatcatg tcgcgcaaaa
     2701 ccatgatcga ggcgatccgc gacgccatgg atgtcatgat gggccgcgac gaaaacgtcg
     2761 tggtgttcgg cgaggatgtc ggctttttcg gcggcgtgtt ccgctgcacc caggggctgc
     2821 aggcgaagta tggcaaaagc cgctgtttcg atgcgccgat caacgagtcg gggatcgtcg
     2881 gcgcggcggt gggcatggcg gcctatgggc tgcgtccctg cgtcgagatc cagttcgccg
     2941 actacatgta tccggcctac gaccagatcg tgtccgaggc ggcgcggctg cgctaccgct
     3001 ccaacggtca gttcacctgt cccatggtgg tgaggatgcc gaccggcggc gggatttccg
     3061 gcggccagac ccatagccaa agcccggaag cgctgttcac ccatgtcgcg ggcctcaaga
     3121 cggtggtgcc atcgacgccg ggcgacgcca aggggctgct gatcgcggcg atcgaagatc
     3181 ccgatccggt catcttcctg gagccgaagc ggctctataa cggcccgttc gacggccatt
     3241 acgaccggcc tgtaacgcca tggtcgaagc acgaactcgg tgaagtaccg gacggttatt
     3301 acaccctgcc gctgggcaag gccgcgatcc ggcgtcccgg ttcggcggtg accgtgctcg
     3361 cctatggcac aatggtctat gtcggcctgg ccgccgccgc cgaaaccggc gtcgatgccg
     3421 agatcatcga tcttcggaca ctggtcccgc tcgacctgga gacgatagaa gcgtcggtcc
     3481 gcaagaccgg caggtgcgtg atcatccacg aggcaaccca cacatcgggc ttcggcgcgg
     3541 agctatcggc gctggtgcag gaaacctgct tctattccat cgaaaaaccg atcaggcgcg
     3601 tcaccggatg ggatacgccg tatccgcacg cgcaggaatg ggactacttc cccggcccgg
     3661 accgggtagg acgcgctcta ctcgaaacga tggagccgta ggatggcgga acaggcgata
     3721 cggctgcccg acgttggcga aggcgtggcc gaggccgaac ttgtggaatg gcacgtcagg
     3781 atcggcgaga tcatccagga agacgcgatc ctggcggcgg tgatgacgga caaggccacc
     3841 gtggaaattc cctcgccgat tgccggcgaa gtcgtttggc tggcggccga agtcggcgat
     3901 gtcgtggccg tcggttccga tctcgtgcgg gtgaaaatcc aaggcggcgg cgccaatccg
     3961 ccggcgccgg aagcgaagcc ccttgccgca tcgcccgccg tcctgccggc gcgggccccg
     4021 accggtggtt cggacacgat cgccccggtg gcccctattc aagtaacggc atcggcgccc
     4081 gatatgccag cccgaaacgc tgggtcgcgg cagcttttgg tcggcgctcc ccgcccggaa
     4141 ggtgagagac cgctggcgtc accggcggtt cgccgacgcg cgcaggaggc cggcatggac
     4201 ctgcggcagg tccccggcac cggcccggtc ggccgcatca cgcaccagga tctggacgcc
     4261 ttcttcgccc gcgggcccgg gatggcgaaa gcgcccggcc tgcagcggcg gacggacgtg
     4321 gaagacgtca aaatcgtcgg cctgcgccgc aggatcgccg aaaaaatggc gatcgccaac
     4381 acccgcatcc cccacatcac ctatgtcgag gaggtggacg tcaccgcgct cgaacaactg
     4441 agggcgacac tgaacagcga cgagcggcac gaccggccca ggctgaccct gcttcccttc
     4501 ctgatgcggg cgatcgtgcg ggcggtggcc gaacagccgg gcctgaatgc gctgttcgac
     4561 gatgaagcgg gcgttctgca tcagcacgaa ggcgtccata tcggcatcgc ggcgcaaacc
     4621 gccggcggcc tgctggtgcc ggtggtccgt catgcggagg cacgggacat ctgggattgc
     4681 gccgtcgagt tgaaccgtct ctcggatgcg gcaaaaaagg caaccgcgac gcgcgacgaa
     4741 ctgacaggtt cgaccatcac gatcacttcg ctcggtgcga tgggcggcgt cgtgacaacc
     4801 cccatcatca atcatcccga ggtggcgatc gtcggcgtca acaaaatgat gatgcgtccg
     4861 gtctgggacg gggcgacatt cattccccgc aagatgatga acttgtcgtc cagcttcgat
     4921 caccgcgtgg tggacggcta cgatgccgcg cggttcatcc agcgcctcaa gacgctgctt
     4981 gaaacccctg cgctgatctt cctggaaggc tgaggccgtg aaagatattt cctgcagatt
     5041 gttgataatc ggcgcgggac cgggcggcta tgtctgcgcc atccgagcgg ggcaactcgg
     5101 cattgacacc gtcatcgtcg aggcgaaaaa gcttggtggg acctgcctga acgtgggctg
     5161 cattccctcc aaggcgctga tccacgcggc cgacgcgttc cacggcatcg ccggctccag
     5221 ttcgctcggc atttcggcag gcaagccgga gctggatctt tcgaaaacaa tgctttggaa
     5281 ggacggcatc gttggccgtc tcaacaacgg tgtcgcagcg ttgctgaaga aggcgggcgt
     5341 gaaggtcgtc gagggctggg cacgattccg cgacgggaag acggtcgagg tggagacgga
     5401 aatcggccag cagataatcc gcgccgaaca ggtggtgatc gccaccggct cgcgtgccgc
     5461 cgaactgccg ttccttccat tcggcggcaa cgtgatctcc tcgaccgggg cattggcgct
     5521 gagtgaagtg ccgaaccggc tggttgtcgt gggcgccggc tatatcgggt tggaactcgg
     5581 cacggccttc gccaaattgg gcgccagcgt gacggtcgtt gaagctgaat cgcgcatcct
     5641 gccgcaatac gacgcggaac tgacgcggcc ggtggcaagg cgcctgcggg aactcggcgt
     5701 cgaggtgctt accggtgcca aggcaaaggg gttcggacgc gatcgcaatg cgctgtctac
     5761 cgaaacggcg gacggagagg gcagggcgct gccgttcgac cgggttctgg tgacggttgg
     5821 ccggaagcca gcgaccgagg gctgggacct ggatgcgatc gacctggaca tggatggccc
     5881 gttccttcgc atcgacgaca agtgccggac gtcgatgcgc ggcgtttatg ccatcggcga
     5941 tgtgacgggc gagccgatgc tggcacaccg ggccatggcg caaggcgaga tggtggccga
     6001 actggtcgcc ggcgaaaacc gctcatggga caagcggtgt attcccgctg tttgcttcac
     6061 cgacccagaa atcgtcaccg ccggattgtc gccggaacaa gccgaggcga ccggcatcaa
     6121 gttcgttacc ggacttttcc cattccaggc aaatggccgc gccatgagcc tggagagcga
     6181 ggctggcttc atccgcgtgg tcgcacgagc cgacaatcac ctgttgctcg ggatccaagg
     6241 cgtgggcagt ggaatcgcgg aactgtcggc gggcttcagc ctggcgcttg agatggcggc
     6301 gcggctcgag gatgtcgctg gaacgatcca tgctcatccc accctgggcg aggggtttct
     6361 ggagagtgtt catatggctt tgggcagccc aattcatatt tagccgatgg aatctgatgc
     6421 ctggtatcca ccgaaccata gtcggtttcc gatccacggc gggcagttac catttgcgtg
     6481 ccgatgtctg gagttgcgcc aaaagtggac ggttcgcgtt tcggcgatag aacggagaaa
     6541 cgaacatggt cgccgtgctc cgataccttc ggcgttcaat ccttctcgaa cattcgcacc
     6601 gggtttgtca ggctggttga ggccctcaac tcacgagctt cagggtagcg gcgtaatcga
     6661 tcttggaacg ggcgaaggct ttctcgcaga gctgaagtag cgcaccgtgt ttataaaggc
     6721 ctcggacgta aagctgattc actttgatat gctgcattgg agtccacgtc tcacggttga
     6781 tcgatggcca ccgcgccgcg atcagcatac gcgcgaagcg gaacctctag ggtaggctca
     6841 caagccgatc gacgcgcggt caaacatacg gaagcgccag aaatagtcgc gccgcgtagg
     6901 gccaagttga cgtcctgctc gctggcaaat ctgcggatct ttgcgaaatt ctcgtagtca
     6961 aacaccaccc tccgccgagt tgggcttcac gactgctggt gctggtcgca aaccggaaat
     7021 acttctgacc gacccgcaga tcctgttgct ctggcttcat aacgccccca gtaccaaccg
     7081 cctagccgtc cccaaaccag atttcggttt cgaagccccg cgcgattact ccccaatgcc
     7141 gctgggaaaa gcagttcagg ttcagcgggt ccatgcgact tccccaggat tcaggccagg
     7201 tggtagcgac cggcgatctc caatctagcg gcttcaggcc aagttacaga ggctgcgcct
     7261 gcccccggta aacgaccact cgcagcgtgc actcggg
//
LOCUS       DASWQN010000980         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1282359, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000980 DASWQN010000000
VERSION     DASWQN010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282359"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VGC82_16775"
     CDS             complement(<1..350)
                     /locus_tag="VGC82_16775"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY0424975.1"
                     /translation="MFPRGATVTQCAVLVGGLGTRLGALTVTTPKPVLPCGGRPFLGW
                     LLREFVRYGVTDFLLLTGHLSAEIERAAADIQATLPRPARIALSQEPERAGTGGAVFH
                     AREQLQARFLLCNG"
     gene            complement(350..1663)
                     /locus_tag="VGC82_16780"
     CDS             complement(350..1663)
                     /locus_tag="VGC82_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY0424976.1"
                     /translation="MTIGLEIAVVLLLILLNGLFSLSELALVSVRRARLAVLERKGVK
                     GAMAARALSDDPLRFLPTVQVGITLVSVLTGVFGGARIASHLQAWIANIPSLAEFAES
                     LALATVVIVTTYLTLVLGELVPKQIALRSPELMAIRVARPIAVVSRILAPAVWLLGVS
                     SNFILRLLGLRRASRQSVTEEELKALLLEGAQTGVLEMEERDMIERVLRLADKPVRAI
                     MTPRTELAWIDRTHPTKAITAALKAAPHSRFVVCDRSIDNVVGVVQAKDLLDRILSGG
                     ELSVSASLRQPMIVPDTISALDALERLKSDALGLALVMDEYGSFEGVVTAADVLQAIV
                     GDASGPEHQEGVPGPEGETSMSLEGMTPVDELKARLNLPDLPAEGSYHTLAGLLLALL
                     RRVPRPGDRIVFGGWRFEVLEMDGRRVDKVLAGREPAAETEISPL"
     gene            1775..2233
                     /locus_tag="VGC82_16785"
     CDS             1775..2233
                     /locus_tag="VGC82_16785"
                     /inference="COORDINATES: protein motif:HMM:NF023338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HEY0424977.1"
                     /translation="MPTIAIPLSVLGLLPFIYFAFGAVGQIPGTAERMLVSLIDYAAL
                     ILSFFGGVHWGLAMAPEAVRPGLRFGAGAVPLVVGWVGLVTAQMVAPSVALAILIVGY
                     LATVLTEHRAAQRLLVPSRYGWMRWGFTIVAVVMMLMVLVLRGVGQTIVF"
     gene            2304..2690
                     /locus_tag="VGC82_16790"
     CDS             2304..2690
                     /locus_tag="VGC82_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424978.1"
                     /translation="MTNPVVSEVTVPGPIVSGSVTAEAGRPTASIIAFPSRVGPVPSR
                     VGPVVPRPDDRLTRAMESLNAAMREQRAALAAWRSSLTALKSTTSGLDESLRQYQSSL
                     NSISGSVSALQAKARALEKWADRAAD"
BASE COUNT          463 a          866 c          876 g          491 t
ORIGIN      
        1 tcaccgttgc acagcaggaa acgcgcttgc agttgctccc gcgcatgaaa caccgccccc
       61 ccggtgcccg ctcgctctgg ctcctgcgac agcgcgatcc gggcgggacg cggcaaggtc
      121 gcctggatat cggcggcggc acgctcgatc tcggccgata gatggccggt cagcagcagg
      181 aaatcggtca cgccgtagcg cacgaactcg cgcagcagcc aacccaggaa cggtcggccg
      241 ccgcagggca ggaccggttt cggcgtggtc acggtcagtg cccctaaacg cgttcccaga
      301 ccgccaacca gcaccgcgca ttgcgtaacg gtggcgcccc gggggaacat caaagcggcg
      361 agatctcggt ttccgcggcg ggttcgcggc cggcgagcac cttgtccaca cggcggccgt
      421 ccatttccag gacttcaaag cgccagccgc cgaacacgat ccggtctccg ggccggggca
      481 cccgccgcag cagtgccagc agcaagcccg ccaaggtgtg gtagctgccc tcggcaggca
      541 ggtcgggcag gttcagccgg gccttcaatt cgtccaccgg ggtcatgccc tccaggctca
      601 tgctggtttc gccttccggg cctggcacgc cttcctggtg ttccggaccg gaggcatcgc
      661 caacgatcgc ctgcaacacg tccgccgcgg tcaccacccc ctcgaagctg ccatactcgt
      721 ccatcaccag agcaaggccg agcgcgtccg atttcagccg ttccagcgca tccagcgcac
      781 tgatggtgtc gggcacgatc atcggctggc gcaggctggc gctgaccgat agttcgccgc
      841 cactgaggat ccggtccagc aggtccttgg cctgcaccac gccgaccacg ttgtcgatcg
      901 accggtcgca gacgacgaac cgcgaatggg gtgccgcctt cagcgcggcc gtgattgcct
      961 tcgtcggatg cgtgcggtcg atccacgcca gctcggttcg cggcgtcatg atggcgcgca
     1021 ccggcttatc ggcgagccgc aggacgcgct cgatcatgtc gcgttcctcc atttccagca
     1081 ccccggtctg ggcgccttcc agcagcagcg ccttcaattc ctcctcggtg acggactggc
     1141 gggaggcccg gcgaaggccc aacagccgca ggatgaagtt ggacgatacg cccagcagcc
     1201 agaccgccgg tgccaggatg cgcgatacca ccgcgatcgg ccgagccacg cggatcgcca
     1261 tcaactccgg gctgcgcagc gcgatctgct tcggcaccaa ctcgccgagc accagcgtca
     1321 ggtaggtcgt gacgatgacg accgtcgcca gcgccaggct ttcggcgaat tcggcgagtg
     1381 aagggatgtt cgcgatccag gcctgcaagt gggatgcgat gcgggcgccg ccgaacacgc
     1441 cggtcaacac gctgacgagg gtaatgccga cctgcaccgt cggcaggaag cgaaggggat
     1501 cgtccgacag cgccctggct gccatcgctc ctttcacccc ctttcgctcc agcaccgcaa
     1561 gccgcgcccg gcgaaccgac accaatgcca gctcgctcaa ggaaaacagg ccgttgagca
     1621 ggatcaacaa caggacgaca gcgatttcga gaccaatggt cattggggct tccggaagga
     1681 cgacgcaaag cgatcaaggc gatttgtagc ggtctatcat aactccgcca cggtcatgag
     1741 gggttgaaag aaggttgcga acaggggaaa gccaatgccg acaatcgcca taccgctcag
     1801 cgtgctggga ctgttgccgt tcatctactt tgcgttcggc gctgtcggac aaattcccgg
     1861 cacggcggag cgcatgctgg tttcgctgat cgattacgcc gcgctgatcc tcagcttctt
     1921 tggcggcgtg cattgggggc tggcgatggc gcccgaggcg gtgcggcccg ggctgcgttt
     1981 tggcgccggc gcggtgccat tggtcgtcgg ctgggtgggg ttggtgacag cgcagatggt
     2041 ggcgccttcg gttgccctgg cgatactgat cgtggggtat ctcgcgaccg tgctgaccga
     2101 acaccgggcg gcgcaacggt tgttggtgcc gtcgaggtat gggtggatgc gctgggggtt
     2161 cacgatcgtc gctgtggtca tgatgctcat ggttctggtt ctgcggggag ttggtcagac
     2221 cattgtattc taatgattta ttgcctcaaa cgggcgttca tgatttatta acgaatgcct
     2281 gttttttgat cggagtgccc gacgtgacga atcccgttgt ttctgaggtt actgtccctg
     2341 gtcccattgt ttccggatcg gtgacggctg aagccgggcg gccgacagcc agcatcattg
     2401 catttccatc gcgtgtcggc cctgttccat cgcgtgtcgg ccccgtcgtg ccgcggccgg
     2461 acgaccgcct gacccgtgct atggagtcgc tgaacgctgc gatgcgggag cagcgcgctg
     2521 ctcttgcggc gtggcgaagt tcgctgaccg cattgaagtc cacgaccagc ggtttggacg
     2581 aaagcttgcg ccaataccag tccagcctga attcgattag cggcagtgtg tcggctttgc
     2641 aggccaaagc ccgcgcgctg gagaaatggg ccgatcgcgc cgcggattaa gggcct
//
LOCUS       DASWQN010000981         2463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1544785, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000981 DASWQN010000000
VERSION     DASWQN010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544785"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VGC82_16795"
     CDS             complement(<1..640)
                     /locus_tag="VGC82_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0424979.1"
                     /translation="MRFRSVRTHLKPMVRRIWGLMYRHLALYRRSWPRLLELAYWPIL
                     QMCIWGFTASFLTARMGNPAIIAGATLLGGVLLWEVTLRSQMGVSISFLEEIWSRNLG
                     HVFVSPLRPFEMVLALIGMSIIRMLLGVMPAILLAWAFYAFNLFALGPVLVLFFVNLM
                     IMGWSVALSVVSLVLRHGAGAEPLAWGVLFGLAPFSAVFYPVSVLPAFLRPIA"
     gene            complement(637..1377)
                     /locus_tag="VGC82_16800"
     CDS             complement(637..1377)
                     /locus_tag="VGC82_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0424980.1"
                     /translation="MNAIEVDNITKQYGNVVAVDQISFVAPVGATVGLLGGNGAGKTT
                     TIAMLLGLLVPTSGRITVLGHDIGKDRFTALARMNFSSPYVALPARLTVKENLRVYGH
                     LYNVRKVEERIAVLSRELDLEPLLDRPAGQLSAGQKTRVALAKALINRPEVLLLDEPT
                     ASLDPDTGDLVRTWLEHYRTESGCTILLASHNMAEVERLCSQVVMLKQGQVVDRGSPD
                     ELLARYGRHDMEDVFLDIARDRRQAEAA"
     gene            1475..2191
                     /locus_tag="VGC82_16805"
     CDS             1475..2191
                     /locus_tag="VGC82_16805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY0424981.1"
                     /translation="MMEFTAAPATLMPGERVYAVGDIHGCLDRLVALHEMIAEDIAAR
                     PVEHVTLVHLGDYVDRGADSAQVIDWLINQPPVPADAFVNLMGNHEHMMLAAVVGADR
                     DAPNLWLMNGGADSLMSWGIARAVPPTEWAGRIPLQHLMFLRDLPVNHRIGPYMFVHA
                     GVRPGVPLDQQSRQDMLWIREPFLSSRVDHGAVIVHGHTPKRQPVVQPNRIAIDTGAV
                     LGGALTCLVLEADRLGFLQV"
BASE COUNT          478 a          775 c          768 g          442 t
ORIGIN      
        1 aggcaatcgg ccgcagaaac gccggcagca ccgaaaccgg atagaacacc gcggagaacg
       61 gcgccaggcc gaacaggacg ccccacgcca gcggctccgc gcccgcgccg tgccgcagca
      121 ccagcgatac cacgctcagg gcgaccgacc accccatgat catcaggttg acgaagaaca
      181 gcaccagcac cggccccaac gcgaacaggt tgaatgcata aaacgcccat gccagcagga
      241 tcgccggcat caccccgagc agcatgcgga tgatcgacat gccgatcagt gccagcacca
      301 tctcgaacgg gcgcagcggg ctgacgaaga cgtggccgag gttgcgcgac catatctcct
      361 ccaggaagga gatcgacacc cccatctggc tgcgcagcgt cacctcccac agcaatacgc
      421 cgcccagcag cgtggcgccg gcgatgatcg ccgggttgcc catgcgcgcc gtcaggaagc
      481 tggcggtgaa gccccagatg cacatttgca ggatcggcca gtatgccagt tccagcaggc
      541 gcggccagga gcggcggtac aacgccagat gacggtacat caggccccag atgcggcgga
      601 ccatcggttt cagatgggtt ctgaccgacc ggaacctcat gccgcttccg cctgcctgcg
      661 gtcgcgggcg atgtcgagga acacgtcttc catgtcatgg cggccgtagc gcgccagcaa
      721 ttcatccggg ctgccgcggt cgaccacctg accttgtttc aacatcacga cctgggaaca
      781 caaacgttcg acctcggcca tgttgtggct ggccaacagg atggtgcagc cgctttctgt
      841 tcggtaatgc tccagccagg tgcggaccag atcgccggtg tcgggatcga ggctggctgt
      901 cggctcatcc agcagcagca cctcgggccg gttgatcaag gcctttgcca gcgcgacacg
      961 ggtcttctgg ccggcggaca actgcccggc gggccgatcc aacagcggct ccaggtcaag
     1021 ctcccgcgac aggacggcga tccgctcctc caccttgcgg acattataaa ggtgaccgta
     1081 aacgcgcaga ttctccttta ccgtcagccg cgccggcaag gcgacatagg gagatgagaa
     1141 gttcatccgc gccagtgccg tgaaacgatc tttgccgata tcgtgcccga gcacggtgat
     1201 ccgaccggag gttgggacca gcaggcccag gagcatggcg atggtcgtgg tcttgcccgc
     1261 accgttgcct ccgagcagcc caaccgtggc gccgaccggg gcgacgaaac ttatttggtc
     1321 cacggccacc acgttgccgt attgtttagt aatattgtcg acttcgatag cattcatgat
     1381 tgatcgcata tggtgctcgc gcgttcacgc caataggcaa tcggccggcg ggggtaattg
     1441 gcgaagccac gatcccggcc tactttgagg catcatgatg gaatttaccg ctgcccccgc
     1501 gaccctgatg cccggagagc gcgtttatgc cgtgggcgat atccacggct gcctggatcg
     1561 tctcgtcgca ttgcatgaga tgatcgctga agatatcgcc gcccggccgg tggaacacgt
     1621 taccttggtg catcttggcg actatgtgga ccggggcgcg gacagtgccc aggtcatcga
     1681 ctggctgatc aaccagcccc cggttccggc cgacgcgttc gtcaacttga tgggcaatca
     1741 cgaacacatg atgttggccg cggtggttgg cgccgacagg gacgcgccaa atttgtggct
     1801 gatgaacggc ggcgccgact cgctcatgag ctgggggatc gctcgtgccg taccgccaac
     1861 cgagtgggct ggccggattc cacttcagca cttgatgttc ctgcgcgacc tgccggtcaa
     1921 ccaccgcatt ggtccgtata tgttcgttca tgcgggtgtc cggcccggtg tgccgcttga
     1981 tcagcaatcg cggcaggata tgctgtggat ccgcgagcct ttcctgtcct cgcgcgttga
     2041 tcacggtgcc gtgatcgtac atggccatac gcccaagcgg cagcccgttg tgcagccaaa
     2101 tcgtatcgcg atcgacaccg gagcggtcct tggcggcgcg ttgacctgcc tcgttctgga
     2161 ggcggacaga ctgggtttct tgcaagtata gacgtgatgt gatcgcgctt gcttgttggc
     2221 agggcggcac gcgaagattg ggcggcacgc aaagattggg cggcaaacaa ggatcggcgg
     2281 aacgtgaaga tgggttgcgc cttcccggcg ggtttgttac ggcaggtcga atgaaccaga
     2341 tcacgcccgg ctccacatcg cgcccagacg agccacagcc ggatctcgcc aaggctgttc
     2401 ggcggctggc ccataccaat gtactggtca tcggcgacgt gatgctggac cgttacacct
     2461 atg
//
LOCUS       DASWQN010000982         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_489865, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000982 DASWQN010000000
VERSION     DASWQN010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489865"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VGC82_16810"
     CDS             complement(<1..524)
                     /locus_tag="VGC82_16810"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HEY0424982.1"
                     /translation="MADKPSLAYLEIMRHIHGGNVYAGFVPTFADDRQGWNSEHAIFD
                     EIIRVTRPTVVIDVGVWKGGSTIYLANLLRQHGVDGVVVGVDTFLGSAERWDRSSGFA
                     GLIPHRFGMPLLYEQFLSNVVRCGAHDRIVPFAQTSTTAAVLLTRLGVQAGLIHIDAS
                     HEQEDVLRDARAYW"
     gene            complement(620..>1935)
                     /locus_tag="VGC82_16815"
     CDS             complement(620..>1935)
                     /locus_tag="VGC82_16815"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0424983.1"
                     /translation="AHAWAASAFVDTACCILAGILEDGPQILLRVPGVANAPGPASIL
                     GTHRRTSVLDAALVNGVASHALDYDDVVGSMGGHPSVMLIPALIPLGEMLGSSGRELV
                     TAYVAGFETVCRIGRGVHHHHYEKGWHPTATLGVFGTVAGAARLLNLSIEHTAIALGM
                     AASFASGLKANFGTMTKPLHVGHAVRDGLFAALMAKEGFTASPGAFDHKQGFLEVFNG
                     PGTYNTARILDGWYDPIECAGGGDPGLKPYPCCGSTHPSIGRMIDLAIRNDLKPERVA
                     RITVLPHARRLPHTDNPDPRTPLAAKFSMQYCVARALADRSVKLSDFEGDAPNDPIVR
                     GLMRRLEARPHPDMPEDWGTEVIVETVDGQRFASRLDDYPSRGPAGNPMTQAELWTKF
                     ADCAGRTLPRAGLPAAFESLMGIACLPCVRDLTSLLRPAQGTRAA"
BASE COUNT          372 a          598 c          649 g          316 t
ORIGIN      
        1 tcccagtaag cgcgagcgtc gcgcagcacg tcctcctgct cgtgggatgc atcgatgtgg
       61 atcaggcccg cctgcacgcc caggcgcgtc agcagcactg cggcggtcgt ggacgtctgc
      121 gcgaagggta cgatccgatc atgcgcgccg caccggacga cgttgctgag gaactgttcg
      181 tacagcaagg gcatgccgaa gcgatgcgga ataaggcccg caaagccgga actacggtcc
      241 caacgttcgg cactgccaag gaaagtatct actccgacca ccaccccatc gacgccatgc
      301 tgccgaagta gattcgccag atagatggtc gagccaccct tccacacgcc cacatcgata
      361 accaccgttg gccgcgtcac acggatgatt tcgtcgaata tcgcatgctc gctgttccaa
      421 ccctgccgat cgtcggcaaa ggtcggtacg aagccggcgt aaacgtttcc gccgtggatg
      481 tgacgcatga tctccagata ggccaggctc ggcttatcgg ccaaattaca gttcctccgg
      541 cgtgatatgc tgacgtcaga atgaacctaa agcattatcg gcaggcgcat gaccagaaaa
      601 tagttgacgg gcgcaatgat caggctgcgc gcgtgccttg agcgggccgc agcagcgagg
      661 tcaggtcgcg aacgcagggg aggcacgcga tccccatcaa cgattcgaac gcagcgggca
      721 ggcctgctcg cggcagggtg cgtccggcgc agtcggcgaa cttcgtccaa agttccgcct
      781 gcgtcatggg attaccggcg gggccgcggc tgggatagtc gtccagacgg gacgcgaaac
      841 gctggccgtc aacggtttcg acaatgacct cggtgcccca gtcctccggc atgtccgggt
      901 gcggcctggc ctccagacgc cgcatcaagc cgcggacgat cggatcattg ggggcatcgc
      961 cctcgaaatc ggacagcttc accgaacggt cggccaaggc acgggccacg caatactgca
     1021 tgctgaattt ggccgcgagc ggggtgcgcg ggtcgggatt gtcggtatgc ggcagccggc
     1081 gcgcatgcgg caggacggtg atgcgggcaa cccgctcggg tttcaggtcg tttcggatgg
     1141 cgaggtcgat catccggccg atggatggat gggtactgcc gcagcaaggg tagggcttca
     1201 agcccggatc gccgccgccg gcacactcga tcgggtcgta ccagccgtcc aggatgcggg
     1261 cggtattgta ggttccgggg ccgttgaaca cctcgagaaa gccctgcttg tggtcgaacg
     1321 cgccggggct ggcggtgaag ccttccttcg ccatcagcgc ggcgaacaat ccatcgcgga
     1381 cggcgtgtcc gacgtgcagc ggcttggtca tggtgccgaa attcgccttc agcccggagg
     1441 cgaacgaggc ggccataccc agtgcgatgg cggtgtgctc gatcgacagg ttcagcaggc
     1501 gggcggcgcc ggcaaccgtg ccgaaaacgc cgagcgtcgc ggtggggtgc cagcctttct
     1561 cgtaatgatg atgatgcacg ccgcggccga tgcggcacac ggtttcgaat ccggcgacat
     1621 aggcggtgac cagctcccgg ccgctgctgc caagcatctc gccaaggggg atcaaggcag
     1681 gaatgagcat aaccgaggga tggccgccca tcgacccgac gacgtcgtcg taatccagcg
     1741 cgtgcgaagc gacgccgttg accaaagcgg catccagcac gctggtgcgc cgatgcgtcc
     1801 caaggatcga tgcgggtccg ggggcgttcg ccacgccagg aacacgcagc aggatttgcg
     1861 gcccgtcctc gaggattccg gcgagaatgc agcaggcggt gtccacaaag gcgctggcgg
     1921 cccacgcgtg ggctt
//
LOCUS       DASWQN010000983         2850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1544834, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000983 DASWQN010000000
VERSION     DASWQN010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544834"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2058
                     /locus_tag="VGC82_16820"
     CDS             <1..2058
                     /locus_tag="VGC82_16820"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0424984.1"
                     /translation="AVVALGALLGSWRATVVAAVSIAVSLAAAVLVFYVRGINLNMMM
                     IAGLLMAVAAVVDDAIADADNAVRRLRETGGEARPVWRIIASAAVEIRGPMLYATLIC
                     VIGVAPLLLMEGLSAQLFQPLAWAYIVAVVISMVVATTVTPALTMLLAPAALPTQQTG
                     SVVMGSLQRLHQRLAGTPMGSPAAAYVLVAGGVLLGVLLWSQQEHTLIPSFKETDVVI
                     ELQAPPGASLQAMERATGSLIHDLRVLPGVRNAAAQIGRALLSHELADVDSAAIWVSL
                     DPKTDYEAALTNMHKVISLHPEIRGDVQTFLSKRMRESVTGEDQAISVRIYGENLDIL
                     RTKAKEIQGILAKIDGVKNPQIEPQAEQQAIDIEVDLDKARAFGLKPGDVRRNASALA
                     GGITVGSLFQEQKVFDVVIWGRPEIRKDLNDIQNMMLDTESGTQVRLGDVARVAVVST
                     PSVIHRQGASRRMDVEADVSGRSLGAVTEEAKRRIKDSAFPFEYHAEVLGEHVERQAA
                     LGSLRSYLVAAAITIVLLLQAALGSWRLAGLIVVGAPVATLGGFVALDLSGGVLSLGA
                     VIGLVAILGLGVRNGIMQIRHLQHLEQQSAEPAGNLVLRGVNDRLPAVLASAITIAAI
                     ALPFAVLGNIAGLEILHPAALVILGGLVTSTILTVFVVPALYPKFVVKSRAKTSTLAP
                     EVA"
     gene            2055..2540
                     /locus_tag="VGC82_16825"
     CDS             2055..2540
                     /locus_tag="VGC82_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424985.1"
                     /translation="MSTSCTASKRGAALVGHLNKAAVAVAVVMAGWQFAVGTARADSP
                     AAPIRETQATIKRLGFGLSQITLPARAAQRLDIKTGEIRDDPLGMRTAPFSSIIYDLD
                     GDAWVYKVTAPLTYVREAVVIEMIKLDHAYLKEGPPAGTQVVTVGVPELYGAEMGVNG
                     E"
     gene            2562..>2850
                     /locus_tag="VGC82_16830"
     CDS             2562..>2850
                     /locus_tag="VGC82_16830"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0424986.1"
                     /translation="MIRWIVGTSLRFRFLVVAFGIGLTYFGAQRLKDIPVDVFPEFAP
                     ARVEVQTLCLGLDSSEVEEQVTVPIENAMNGVPGVEILRSSSVPQLSSVTAI"
BASE COUNT          495 a          852 c          963 g          540 t
ORIGIN      
        1 gcggtggtcg cactcggcgc gctgctcggt agctggcgcg cgaccgtcgt ggcggcggtg
       61 tccattgccg tctcgctcgc cgcagccgtc ctggtgttct acgtgcgcgg catcaacctg
      121 aatatgatga tgatcgccgg cctgctgatg gcggtcgccg cggtggtcga tgatgccatc
      181 gcggatgccg acaatgccgt gcggcgcctg cgcgagactg gcggggaggc caggccggtg
      241 tggcgcatca ttgccagcgc cgcggtggag atccgcgggc cgatgctcta cgccacgctg
      301 atctgcgtga tcggggtggc cccgctcctg ctgatggaag ggttgtcggc gcaattgttc
      361 cagccgctcg cgtgggccta catcgtcgcc gtggtgatct cgatggtggt cgccaccacc
      421 gtcacgccgg ctctgacgat gctcttggct ccggccgcgc tgccgacgca acagaccggg
      481 tcggtggtca tgggcagcct tcagcgcctc caccagcgcc tggccggcac gccgatgggt
      541 tcgcccgccg ccgcgtatgt gctggtagca ggcggcgtgc tgctcggcgt gctgctttgg
      601 tcgcaacagg aacacacgct gattccgtcc ttcaaggaga ccgatgtcgt catcgagctg
      661 caggcgccgc ctggagcatc cttgcaggcg atggagcgcg cgaccgggtc gctgatccat
      721 gatctgcggg tacttccagg cgtgcgcaac gcggcggcgc aaattggccg tgcgctattg
      781 tcgcacgagc tggctgacgt cgactcggcc gcgatctggg tgagtctcga tccgaaaacc
      841 gactatgaag ccgcgctgac gaacatgcat aaggtcatca gcctccatcc cgagatccgc
      901 ggcgatgtgc agaccttcct ttccaagaga atgcgcgaga gcgtaaccgg cgaagatcag
      961 gcgatctcgg ttcggatata cggcgaaaat ctcgacatcc tccgcaccaa ggcaaaggag
     1021 atccagggga tcctggcaaa gatcgacggg gtcaagaacc cgcagatcga gccacaggcc
     1081 gaacagcagg cgatcgacat cgaagtcgat ttggacaaag cccgcgcgtt tggcctcaag
     1141 cccggtgacg tccgccgcaa tgcgtcggcg ctggccggtg gcatcacggt aggcagcctg
     1201 tttcaggagc aaaaggtgtt cgatgtcgtg atctggggac gtccagagat ccggaaggac
     1261 ctcaacgaca tccagaacat gatgctggac actgaatccg gcactcaggt gcggcttggg
     1321 gatgtcgccc gcgtggctgt ggtgtccacg ccaagcgtga tccatcggca aggtgcgtca
     1381 cggcggatgg atgtcgaggc cgatgtcagc ggccggtcgc tcggcgccgt gaccgaggaa
     1441 gccaagcgcc gcatcaagga cagtgcgttt ccgttcgaat atcatgcgga agtgctcggc
     1501 gagcatgtcg agcggcaggc cgccctggga tcgttgcgca gctacctggt tgccgcggcg
     1561 atcaccattg tcctgctgct acaggctgcg ctgggaagct ggcgtctggc cgggcttatc
     1621 gtggtcggcg cgccggtggc gacgctgggc gggttcgtcg cgctggatct aagcggcggg
     1681 gtgctttcgc tcggcgcggt gattggattg gtcgcgatct tgggtcttgg cgtgcgtaac
     1741 gggatcatgc agatccggca cctgcaacac ctcgaacagc aatcggctga gccagccggc
     1801 aacctggtcc tgcgcggggt gaacgaccgg cttcccgcgg tgcttgccag cgcgataacc
     1861 attgccgcga tcgctctgcc cttcgcggtg ctcggcaaca tcgcggggct ggagatcctg
     1921 catccggcgg ccttggtgat ccttggcggc ctggtgactt ccacaattct cacggtgttc
     1981 gtggttccgg cactttatcc gaaattcgtg gtcaagagcc gtgccaagac ctccacgttg
     2041 gctccggagg tcgcatgagc acgtcctgca cggcgtcaaa gcggggggcc gccttggtgg
     2101 ggcacctgaa caaagccgct gttgcggtgg cggtggtaat ggcgggttgg caattcgcgg
     2161 tgggaaccgc gagggccgac tctcccgccg cgccgatccg cgagacccag gcgacgatca
     2221 agcgcctcgg atttggacta agccagatca ccttgccggc gagggcggcc cagcggctcg
     2281 acatcaagac aggcgagatc cgcgacgacc cgttaggcat gaggactgcg ccgttctcgt
     2341 cgatcatcta cgacctcgac ggggatgcct gggtgtacaa ggtcacagcg ccactgacct
     2401 atgttcgcga ggcagtcgtg atcgaaatga tcaaactgga ccacgcctac cttaaggaag
     2461 gtccgccagc cgggacgcaa gtcgtaacgg tcggcgtgcc ggaactgtac ggggcagaaa
     2521 tgggcgttaa cggcgaatag ctgggtgagg attgccaagc catgatcagg tggatagtcg
     2581 ggactagcct gcgttttcgt ttcctcgtcg tagcattcgg catcggactc acctatttcg
     2641 gtgcccagcg cctgaaggac atcccagtcg atgtctttcc ggagttcgcg ccggcccgag
     2701 tggaagtaca gacgctatgc cttggacttg attcgtccga ggtggaggag caagtcacgg
     2761 tgccaatcga gaacgccatg aacggcgttc ctggcgtcga gatattgcga tcaagttcgg
     2821 tgccgcagtt atcctcggtc accgcgatct
//
LOCUS       DASWQN010000984         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_886785, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000984 DASWQN010000000
VERSION     DASWQN010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886785"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VGC82_16835"
     CDS             <1..629
                     /locus_tag="VGC82_16835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0424987.1"
                     /translation="RPIPQLNWVRTVLHGIPADLLTPLPVKQEYGAFLGRISPEKGVE
                     RAIKIAGKAGMKLKIAAKVDNADKEYYDSQIKPLIRGNPDVEFIGEINDAQKPEFLSG
                     AHALIFPIDWPEPFGLVMIEAMACGTPVIAFNCGSVPEVMDEGVTGFVVDDIDQAVAA
                     IGKIHTLDRAQVRATFDRRFTARRMAEDYVDVYQTLARPVRRLHMVSA"
     gene            complement(803..1207)
                     /locus_tag="VGC82_16840"
     CDS             complement(803..1207)
                     /locus_tag="VGC82_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0424988.1"
                     /translation="MSADVFLDTNILVYGVVQGDARTPRAESLLRQGCTISVQVLNEF
                     TNVARRKLRRTWPEINQAVGALRVLFPEPKAILPATHAAALVIAQREGFAFYDSLIIA
                     SAIEAGCSTLLSEDMQSGRVIADGLTIRNPFL"
     gene            complement(1197..1427)
                     /locus_tag="VGC82_16845"
     CDS             complement(1197..1427)
                     /locus_tag="VGC82_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY0424989.1"
                     /translation="MQIGKWGNSLAVRLPSAVVDALGLREGDDIEIEVAGSRNFKIGR
                     DHRKDDAIERLKALDWSLPPGFRFSRDEANER"
     gene            complement(1498..2136)
                     /locus_tag="VGC82_16850"
     CDS             complement(1498..2136)
                     /locus_tag="VGC82_16850"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY0424990.1"
                     /translation="MPDFFEILGTTRSMRRLKPDPVPDELIKKILEAGTAAANGGNNQ
                     TWRFLVIKDPAIKKAVQVWYKKAFEEVVGPRYATSAPPPGVTGERYSRQHGAVEYLTE
                     HFHEAPVWIVACLQDGPNPTRGAGASIYPAVQNMLLAIRALGLGSTLTTRHLLHEKEA
                     EAALGLPPGVHSYAILPIGYPMGKFGPVGRGKLSDFVFQDKWGQPYPAVAEA"
     gene            complement(2152..2949)
                     /locus_tag="VGC82_16855"
     CDS             complement(2152..2949)
                     /locus_tag="VGC82_16855"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0424991.1"
                     /translation="MDFTGKVALITGGGGGIGRATALGFALRGAKVMVVDADADSGRA
                     SVDIIAQRGGTAAFAQADVTKSGSVKDYVEKSLALYGRIDAFFNNAGIEGKVMPTQDY
                     DEDVFDQVIAVNLKGVFLGLRHVLPVMLKQGSGAIVNTASVAGLFGSPGMAAYVASKH
                     GVLGLTKVASTDVAALGVRVNAVCPGPVETRMMRSLEQQRNPGNPEGVHAAYAAGIPT
                     GRYAQPEEIAGAVLYLCSDLSGDVTGTHIVIDGGRSGSGGVAPAKRI"
     gene            complement(2989..>3739)
                     /locus_tag="VGC82_16860"
     CDS             complement(2989..>3739)
                     /locus_tag="VGC82_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0424992.1"
                     /translation="GGLMSITGEPGSGPMRVGIPVADLTAGIFAAMGILVALLEREQS
                     GEGQWVKSSLLGAQISMLDFQAARWTIGKEVPGQAGNNHPTSIPTGVFATADGYINIA
                     AAGDDIYRRLCKALGAEHLAGDPMYATGRDRSQHRDALNEAIGAITRGKTSADWIEML
                     NKAGVPSGPIYKMNEVFADPQVKHLGMTRKVPHKVLGEVEVVGQPIELSRTPWSVRTA
                     TPEAGEHSDAILAELGYSAAEIAALRAAKAV"
BASE COUNT          657 a         1201 c         1145 g          736 t
ORIGIN      
        1 ggcgcccaat cccgcagttg aattgggtac gcaccgtctt gcacggaatt cccgccgatc
       61 ttttgacccc gcttccggtc aagcaggaat acggcgcgtt cctcggccgc atttcgccgg
      121 agaaaggcgt cgaacgcgcc atcaagatcg ccggcaaggc cgggatgaag ctgaagatcg
      181 ccgccaaggt ggataacgcg gacaaggaat actacgacag ccagatcaag ccactcatcc
      241 gaggcaaccc cgacgttgag ttcatcgggg agatcaacga cgcgcagaag cccgagttcc
      301 tttctggcgc gcacgccttg atctttccga tcgactggcc ggagcccttc ggtctggtga
      361 tgatcgaagc catggcgtgc ggcacgccgg ttatcgcctt caactgcggg tcggtgcccg
      421 aggtgatgga cgaaggtgtc accggatttg ttgttgatga catcgaccag gcggtggccg
      481 cgataggcaa gatccatacg ctcgaccgcg cccaggtccg ggcgacgttc gatcgccggt
      541 tcacggctcg gcggatggct gaggactatg tggacgttta ccagaccctt gcgcggccgg
      601 tgcgacgcct gcacatggtc agcgcctgaa gatctctgtt ccgtcgcgtg ccggccgccc
      661 cagttaacga gccccagtta acgatatgtc cggcagcgac gtgtctggcg gcgtgccggc
      721 catcgatgat tttcccgcgt gcggacaaac ccgtgcatgg tcgcacaggc gcggccacga
      781 cactcgtttc tgattactgt ggctacagaa acggattccg aatcgtcaat ccatcggcga
      841 tgacccggcc actttgcata tcttccgaca gcagcgtcga gcatcctgcc tcgatcgccg
      901 atgcgatgat cagggagtcg tagaatgcaa aaccctctcg ctgtgcgatt accagggctg
      961 ctgcatgtgt ggcgggtaga atcgccttcg gttcgggaaa cagcacccgc aacgctccca
     1021 cggcctgatt gatttcaggc caggtacgcc tgagttttcg ccgcgccaca ttggtaaact
     1081 cgtttaacac ctgtacgctg atcgtacaac cctggcgaag cagcgactcc gcgcgcggcg
     1141 tccgcgcatc gccttggacg accccataca ccaggatatt ggtgtccaga aaaacgtcag
     1201 cgctcattgg cctcatcgcg gctgaaccga aagccgggag gcagggacca gtccagtgcc
     1261 ttcaaccgct cgatggcatc gtctttacga tgatcccgac cgattttaaa gtttcggctt
     1321 cccgccacct cgatctcgat gtcgtcgcct tcccgcaagc cgagcgcgtc cacgaccgcg
     1381 gagggaagcc gaacggccaa actattgccc cattttccga tctgcatgtc gctgcctcct
     1441 gatatacatt tagatcggta tatcattatt tgcaagcttt ctggaaggcg ggggctgtca
     1501 ggcctccgcc accgccggat aaggctggcc ccacttatcc tggaacacga agtccgacag
     1561 tttgccacgg ccaaccggcc cgaacttgcc catcggataa ccgatcggca ggatcgcata
     1621 ggaatgcacg cccggcggca aaccgagcgc ggcttcggct tccttttcgt gcagcaggtg
     1681 ccgtgtcgtc agcgtcgagc ccagccctag cgctcggatt gccagcagca tgttctgcac
     1741 cgccggatag atcgaggcgc cggcgccacg ggtcgggttc ggtccgtcct gcaggcaggc
     1801 gacgatccac accggtgcct cgtggaagtg ttcggtcagg tattccaccg cgccgtgctg
     1861 gcggctgtac cgttcgccgg tcacccctgg tggcggagcg ctggtggcat agcgcggccc
     1921 gacgacctcc tcgaaggcct tcttatacca gacctggacc gccttcttga tcgccgggtc
     1981 cttgatcacc aggaatcgcc atgtctggtt gttgccgccg tttgccgcgg cggtgccggc
     2041 ttcgaggatc tttttgatca gttcgtccgg cactgggtcc ggcttcaggc ggcgcatcga
     2101 gcgggtggtt ccgagaatct cgaagaaatc gggcatgatt tacgttctcc cctaaatacg
     2161 ttttgccggc gcgacgccgc ccgaaccgga acggccgccg tcgatgacga tatgcgtgcc
     2221 tgtcacgtcg ccggacaggt cggaacacag gtacagcacg gcgccggcga tttcctccgg
     2281 ctgggcgtag cgtccggttg gaatgccggc ggcgtaggca gcgtggacgc cttcgggatt
     2341 gccgggattg cgctgctgtt ccagcgatcg catcatgcgg gtctcgaccg ggccggggca
     2401 taccgcgttg acccgcacgc ccagggccgc cacgtcggtg gacgcgacct tggtcagccc
     2461 gaggacgccg tgcttagagg cgacataggc ggccattccc ggtgatccga acaatcccgc
     2521 gaccgacgcg gtgttcacga tcgccccgga accttgcttc aacatcaccg gcagcacatg
     2581 acggagaccc agaaagacgc ctttcaggtt gaccgcgatg acctggtcaa agacgtcctc
     2641 atcgtaatcc tgggttggca tgaccttgcc ttcgatgccg gcgttattga agaatgcgtc
     2701 gatccggccg tatagcgcga gagacttctc gacgtagtcc ttgaccgaac ctgatttcgt
     2761 tacgtcggct tgcgcgaagg cggctgtgcc gccgcgctgg gcgatgatat cgacgctggc
     2821 ccggccggaa tccgcatcgg cgtccaccac catgacttta gcgccccgca gcgcaaaacc
     2881 aagggcggtt gcccggccga tcccaccgcc gccgccggtg atcaaagcca cctttccggt
     2941 gaagtccatt gttgtcccct cccttgtaat gtcagcctgg cacgtgagct aaacggcttt
     3001 ggcggcgcgc agtgctgcga tttccgccgc gctgtagcca agttcggcca gaatggcatc
     3061 gctgtgctcg cccgcctcgg gcgtcgcggt gcgaacactc cagggtgtgc ggctgagctc
     3121 gatgggctgg ccgacgacct ccacttctcc cagcaccttg tgcggaacct tgcgggtcat
     3181 tcccaggtgc ttcacctgag gatcggcgaa gacctcgttc atcttgtaga tcgggcctga
     3241 cggaaccccc gctttgttca gcatctcgat ccaatcggca gaggtctttc cccttgtaat
     3301 cgcaccgatc gcctcgttca gcgcgtcccg gtgctgcgac cggtcgcgtc cggtcgcata
     3361 catcggatcg ccggcgaggt gttccgcacc cagcgccttg cagagccggc gatagatatc
     3421 gtcgccggcc gcggcgatat tgatgtagcc gtcggcggtg gcgaacacgc cggtcgggat
     3481 gctggtgggg tggttgttgc cggcctggcc gggtacttcc ttgccgatcg tccagcgcgc
     3541 ggcctggaag tccagcatcg agatctgggc acccagcaac gagcttttca cccactggcc
     3601 ttcgccggat tgctcgcgct ccagcagggc gaccaggatg cccatggcgg cgaagatgcc
     3661 ggcggtgagg tcggcaacgg ggatgccgac gcgcattggg cctgaccccg gctcgccggt
     3721 gatcgacatc aggccgccc
//
LOCUS       DASWQN010000985         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1942148, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000985 DASWQN010000000
VERSION     DASWQN010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942148"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1322)
                     /locus_tag="VGC82_16865"
     CDS             complement(93..1322)
                     /locus_tag="VGC82_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010994077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEY0424993.1"
                     /translation="MIDERAIEERYAALRDQLSAKRAQRLDERGRRLYAAAEVRVIGY
                     GGLAAVARATGISRGTIGRGLKDLDRGPLPHGRVRRDGGGRHPLSASDPTLLEDLREL
                     IEPVTLGDPVRPLRWVSKSHKKLAAALRAQGHTVSPNTVRKLLPQLGYSRQANRKAND
                     GRQHADRDAQFEPINAQVRAFQGDDQPVISVDTKKKELVGNFSNKGTDYRPEGQPRRT
                     EVHDFEKKDLGKVVPYGVYDLADDSGWVSVGITSDTAQFAVNTIGCWFEKMGRERYPA
                     ASRLLITADCGGSNGARVRLWKRELQALADATGLSITVCHYPPGTSKWNKIEHRLFCH
                     ITQNWRSKPLTSRLAVVELIAATTTTTGLTVACELDTNTYEKGIKVTKAEMATLNLHR
                     DAFHPEWNYTIEPRPST"
BASE COUNT          286 a          534 c          578 g          359 t
ORIGIN      
        1 tggcagatgc gattactctg acgaacagta tcactaacct tgttatattg aagcagccaa
       61 ttgcgattag ggcaggcgca ggaataaccg ggtcaggttg atggccgtgg ttcgatggtg
      121 tagttccatt ccggatggaa ggcgtcgcgg tgtaggttaa gggtggccat ttcggccttg
      181 gtcaccttga tgcccttttc ataggtgttg gtgtccagct cgcacgcgac agtgagcccg
      241 gttgttgtgg tcgtggcggc gatcagttcg accacggcca gacggctggt cagcggcttg
      301 gaccgccagt tttgcgtgat gtgacagaag agccggtgtt cgatcttgtt ccacttcgag
      361 gtgcccggcg ggtagtgaca taccgtgatg ctcagcccag tggcgtcggc cagcgcttgg
      421 agttcgcgct tccacagtcg cacacgggcg ccattggagc cgccgcaatc ggcggtgatc
      481 aacagccggg atgcggcggg ataacgctcc cgccccatct tctcaaacca gcagccgatg
      541 gtgttgacgg cgaactgggc ggtgtcgctg gtgatgccga cgctcaccca gccgctgtcg
      601 tcggccaggt cgtagacgcc gtagggtacc accttgccca agtccttctt ttcgaagtca
      661 tgcacctcgg tccggcgcgg ctggccctcg ggacggtagt ccgtcccttt gttgctgaag
      721 ttgccaacca gctctttctt cttggtgtcg accgagatca ccggctgatc gtcgccttgg
      781 aaggcgcgga cctgggcgtt gatgggctcg aactgggcgt cgcgatcagc atgctgacgg
      841 ccgtcgttgg ctttgcggtt ggcttggcgg ctgtagccga gctgcggcag cagcttacgc
      901 accgtgttcg ggctgaccgt gtgcccctgc gcccgcaggg cggcggcgag tttcttatgg
      961 ctcttggaga cccaccgcag cggccgcacc gggtcaccca gggtgaccgg ttcgatcagc
     1021 tcgcgcaggt cctccagcag ggtgggatcg ctggcgctca gcgggtgccg gccgccaccg
     1081 tcgcgccgta cccggccgtg gggcagcggg ccgcggtcga gatccttcag cccccggccg
     1141 atcgtgccac gcgatatgcc ggtggcccgc gccaccgccg ccagaccgcc gtagccgatg
     1201 acacgcactt ccgccgccgc atagaggcgt cgtccccgct catccaagcg ctgagctcgc
     1261 ttagcgctga gctggtcgcg caacgccgca tagcgttcct caatggctcg ttcgtcgatc
     1321 atccggccaa acatagaagc cgccgacgct ctggaaaccc catcaaccga tccgacccaa
     1381 atcagagccg gttattcctg cgcgtcgcct aaaggagcgt gccggatgct acggtggtgg
     1441 agccagcgtg ccgggtgacg cggtattcgc cgatctcttc catgtctccc tccttcagtg
     1501 cctgtccagc agatggtgct caggcactgc ttgctgaacc cgcagaaacc ctggacttcc
     1561 gcgtcgttat atctaataat atataacgct ttatcagagg acggaggaca ccatggcgga
     1621 tgtggagcag atgctggcgc ggcgcacggc gatcgctgag gaaatgactg gactgatcgg
     1681 ccggccgggt gtgctggtgc gtgaggagaa ggtgctggtc ggcgatctgc gacgcgagat
     1741 ggcgcggtcc ggcgtca
//
LOCUS       DASWQN010000986         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_886951, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000986 DASWQN010000000
VERSION     DASWQN010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886951"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1217)
                     /locus_tag="VGC82_16870"
     CDS             complement(123..1217)
                     /locus_tag="VGC82_16870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0424994.1"
                     /translation="MKIAIYGSSLLSSYWNGAATYYRGILRALASYGHAITFFEPDAY
                     DRQSHRDIAPPSWCEVVVYPATETGLHDVLARAAAADVVIKASGVGVYDEALLDGVVA
                     LSDPNALRIFWDVDAPATLAEIAAAPDHYLRAVLPRLDTVLTYGGGNPVVELYRALGA
                     PDCVPVYNGLDPETHHPVPSEDRYRADLTFLGNRLPDREQRVADFFLSAAEQLSDRPM
                     CLGGSGWEDKLCPPNVVKLGHVPTGDHNALNVSALAVLNISRDSMAATGFSPATRIFE
                     AAGAGACLLTDPWEGIDHFLAPGKEVLVVRDGADVAEAVHSLTPLRAATIGLAALRRV
                     LAEHTYDQRAATLHALLTRLTAKRRSERAA"
     gene            complement(1231..>2083)
                     /locus_tag="VGC82_16875"
     CDS             complement(1231..>2083)
                     /locus_tag="VGC82_16875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0424995.1"
                     /translation="VHEWTPPELMARIGRLRHTGGRFVLLFHDTHHRSVSDAGAIHAL
                     PLTDYDAVLAFGETVRSRYERLGWGPRAFTWHEAADTLVFRPQSETASGAGQRDDIVW
                     IGNWGDGERAQELSDFLIEPARGLGLKGTVHGVRYPPQALQHLTGTGLRYRGWIANAD
                     VPTAFARHRMTVHVPRRPYVEALPGIPTIRIFEALACGIPLISAPWHDEEGLFQPGSD
                     FLVARNSAEMTQHLDALRHDPDLAGSLAASGLQRIQQRHTCVHRVDELFAIVAAIRQR
                     KPLEAPE"
BASE COUNT          385 a          653 c          689 g          356 t
ORIGIN      
        1 cagcaccctc tggcccatgc tggcgaggcc gcgcaagagt gcgcgatagg tcgtggcgtg
       61 gccatttccc cacgaagaac tcagggacaa tccgaggacg atcagatcat ccggccggct
      121 gctcatgccg cacgttccga acgacgcttg gcggtcagac gggtgagcag cgcgtgcaga
      181 gtcgcggcgc gctggtcata agtgtgttcc gcgagcacac ggcgcagggc ggcaagtcca
      241 atcgtcgcgg cacgcagtgg ggtcagcgag tggaccgcct ccgccacgtc cgcgccgtcg
      301 cggaccacca gcacttcctt accgggagcc aggaaatggt cgatcccctc ccagggatcg
      361 gtcaacaagc aggcaccggc gccggccgcc tcgaagatcc gcgtcgccgg cgagaaaccg
      421 gtggcggcca tgctgtcgcg gctgatgttc aagaccgcca gggcgctcac gttgagtgcg
      481 ttatgatccc ccgtcggcac atgccccagt ttgacgacgt tcggcggaca aagcttgtct
      541 tcccagccgg agccgccaag gcacatcggg cgatcggaaa gctgttcggc tgccgacaga
      601 aagaagtcgg cgacgcgttg ctcgcggtcc ggcagccggt tgccgagaaa cgtcaggtct
      661 gcgcggtagc gatcctcgga aggcactgga tgatgcgttt ctggatcaag tccgttatag
      721 accggtacgc aatccggcgc accgagtgct cggtataatt cgacgaccgg attgccgccg
      781 ccgtaggtga gtacagtgtc cagtcgcggc aatacggcac gtaaataatg atctggtgcc
      841 gccgcgattt cagccaaggt cgccggtgcg tccacgtccc agaaaatgcg cagagcattt
      901 gggtcagata gcgccaccac gccgtcgagc agtgcctcgt catagacgcc aaccccgctg
      961 gccttgatga ccacatccgc cgcggcggct cgggcgagga cgtcatgcag gccggtttcc
     1021 gtcgcaggat acaccaccac ttcgcaccat gacggcggcg cgatgtcgcg atggctttgc
     1081 cggtcgtatg cgtccggctc aaagaacgtg atggcgtggc catacgacgc cagtgcccgc
     1141 aggatgccgc ggtagtaggt cgctgcaccg ttccaatagg acgacagcag gcttgagccg
     1201 tagatcgcga ttttcattga acggctattt tcattctggg gcctcaagcg gcttgcgctg
     1261 ccggatcgcg gcgacgatgg caaacaattc gtccacgcga tgaacgcagg tgtggcgttg
     1321 ctgaatgcgc tgaagaccgc tggcggccaa gctgccggca aggtcgggat cgtgccgcaa
     1381 tgcgtccagg tgctgtgtca tttcggcgga attccgagca accaggaaat ccgatcctgg
     1441 ctggaacaag ccttcttcgt cgtgccaggg ggcgctgatt agcgggatac cgcaggccag
     1501 tgcctcgaag atgcgaatgg tggggatccc cggcagcgcc tccacgtagg ggcggcgggg
     1561 cacatgcacc gtcattcgat gccgtgcgaa ggcggtgggg acgtccgcat tggcgatcca
     1621 gccacgatac cggaggccgg tcccggtgag atgctgaagt gcctgcggtg ggtagcggac
     1681 accgtggacg gtacctttca agcccaggcc gcgagccggt tcgatcagga aatccgatag
     1741 ctcctgcgcg cgttccccgt cgccccagtt gccgatccac acgatatcgt cgcgctggcc
     1801 ggcacctgaa gcggtttcgg attgcggacg gaataccagc gtgtccgccg cctcgtgcca
     1861 ggtgaaggct cggggccccc agcccagccg ctcataccgc gaccgcaccg tctcgccgaa
     1921 tgccagaacc gcgtcatagt ctgtaagcgg cagcgcgtgg atggcgcctg cgtcggacac
     1981 gctgcggtgg tgcgtgtcgt ggaacaacag cacaaagcga ccgccggtgt gccgcaagcg
     2041 gccgatccgc gccatcagtt ccggcggtgt ccattcgtgg acg
//
LOCUS       DASWQN010000987         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2206411, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000987 DASWQN010000000
VERSION     DASWQN010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2206411"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..340)
                     /locus_tag="VGC82_16880"
     CDS             complement(155..340)
                     /locus_tag="VGC82_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424996.1"
                     /translation="MFRLAISLAGFIVTAAMHPAIADPASAVPPAAAQYAPASEQPAQ
                     VQVPVSDINAPVGAGWG"
     gene            complement(430..1812)
                     /locus_tag="VGC82_16885"
     CDS             complement(430..1812)
                     /locus_tag="VGC82_16885"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0424997.1"
                     /translation="MPIDHIVVLMMENNGFDRMLGWMGGAGRDVDGVDPSHPLSNPDQ
                     SDGGEVLQGETRTRNIQRDPLHYLANSIAQLDGGTNRGFVNDYVRTYPSCTAEERREI
                     MAWYPRGFLPALHTMAEHGVVFDRWFASMPGPTWMNRLFAHAGTSLGHVAEPGSLFNA
                     NLHIYDERTLYDELSDAGIPWHIYFGDVPQSLVLTHQWRHLRNYRAFKHWEADVKAGK
                     LPAYSFIEPTYFGPHQNDQHPPHDVMRGDALIAQVYNPLRANQALFERTLLIILYDEH
                     GGFFDHVVPPKTVPPDDHTAEFAFDRLGFRVPAIFVSPLLDPGVAHAVYDHTSLLKMA
                     AEKWPDVKPIGRRARESNDPLAELKWRTQPRTDLPEAPIAPDIQPASALSGLQGFKAS
                     LFGLSHHLESLIDHAGHRGALMTRAHEALHDSHAGGRLVTDRIEAFLSDRMRGRGLFG
                     RLFGGNKSRR"
BASE COUNT          387 a          591 c          685 g          407 t
ORIGIN      
        1 tgggtgatgt tgcacgggct gcaacccggc aacccacaca attttctcga aatcacctgc
       61 ggtttcctcc gatggcctcg gatgatcgag aaggattccg ctgagcaatg ccaacagcaa
      121 cgcacttccg ggaatgataa acgtcatcat ctgactagcc ccagccggcg ccaaccggcg
      181 cgtttatgtc cgataccgga acctgtacct gtgcgggctg ctcgctggcc ggagcatatt
      241 gtgcggcggc aggtggtacg gctgaagcag ggtcggcgat cgcgggatgc attgctgcgg
      301 ttacgatgaa accggccaac gagatagcta agcggaacat ctgatttctc ctgtgtgctc
      361 gatcgttgga cacaggttat ccgcggccgg acgtcatcgc tgtgaagcgg ctcacatgga
      421 cgaaatactc tatcggcggg acttgttgcc gccgaaaaga cggccaaaaa ggccccggcc
      481 cctcatgcgg tcggacagaa aggcttcgat ccggtcggtc accaaccggc ccccggcgtg
      541 gctgtcgtgc agtgcctcgt gcgcgcgggt catcaatgca ccacgatggc cggcgtggtc
      601 gatcaggctt tccagatggt ggctcaggcc aaacaaagac gctttgaatc cttgaagacc
      661 cgacagggca ctggccggtt gaatgtcggg ggcgatcgga gcctccggca ggtcggtgcg
      721 gggctgggtc cgccatttca gttcagccaa cgggtcattg gattcccggg cgcggcggcc
      781 gattggcttt acgtcaggcc acttttctgc cgccatcttc agcaggctgg tatgatcgta
      841 aaccgcgtgc gccacgccag gatcgagcag cggcgatacg aaaatcgccg gcacgcgaaa
      901 gccgaggcgg tcaaacgcga attccgcggt gtggtcgtca ggtggaaccg tctttggcgg
      961 gacgacgtgg tcgaagaaac caccatgttc atcgtatagg atgatcagca acgtgcgctc
     1021 gaacagcgcc tggtttgccc gaaggggatt gtaaacctgt gcgatcaggg catcgccgcg
     1081 catgacgtcg tgcggcggat gctggtcgtt ctgatgcggg ccaaaatagg tcggctcgat
     1141 aaagctgtaa gcgggcagct tgccggcttt cacgtcggct tcccagtgct tgaaggcgcg
     1201 atagttgcgt agatggcgcc actgatgggt tagcaccagc gattgcggga cgtcgccgaa
     1261 atagatgtgc cacgggattc ccgcatcgct gagttcgtcg tatagcgtgc gttcgtcgta
     1321 gatgtgcagg ttggcgttga acaggctgcc gggttccgcg acatgaccca ggctggtgcc
     1381 ggcgtgggca aacagccggt tcatccaggt cggtcctggc atcgaggcaa accagcggtc
     1441 gaataccacg ccatgctctg ccatcgtatg cagcgcgggc aagaagccgc gcggatacca
     1501 cgccatgatt tcgcggcgtt cctcggctgt gcagcttgga taggtgcgga cgtaatcgtt
     1561 gacgaagcct cgattggtgc cgccgtcgag ttgggcgatg gagttcgcca gatagtgcag
     1621 cgggtcacgc tggatgtttc gggtccgcgt ttccccctgc aacacttcgc cgccgtcgct
     1681 ttggtcgggg ttagacaggg gatgggaggg gtccacgcca tcgacgtctc gcccagcgcc
     1741 gcccatccaa ccgagcatgc ggtcgaaacc gttgttttcc atcatcagga cgacgatgtg
     1801 gtcgatgggc atggtctgct ccgtggtagc cgaaggtagg gcgccgctgg ggtggtttgg
     1861 ccggcggcag ccatcgaggc gagcgcccgg gatcagcgaa ggatatagcg cctctaccag
     1921 tttctgcgaa gttgcgtcag agcgttcgat tgacggcggt ctctccgaga cgacccgccg
     1981 tggcccgagg gaagtggcgc gctccgaagg ccctacagcc gcgccttgcg cgctttcagg
     2041 ccgactggaa aagcggcgag ccagtttgcc
//
LOCUS       DASWQN010000988         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_753366, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000988 DASWQN010000000
VERSION     DASWQN010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753366"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1752)
                     /gene="trpE"
                     /locus_tag="VGC82_16890"
     CDS             complement(223..1752)
                     /gene="trpE"
                     /locus_tag="VGC82_16890"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEY0424998.1"
                     /translation="MNVLVTPGFAVFRLAYEAGRGSLVWRKTVADLETPVAAFLKLAH
                     GQPNSFLLESVEGGAARGRYSIIGMQPDLVWRCQDGRASVNRYALSAPHAFVAEAKPP
                     LDSLRALIAETQLEVPAHLPPMAGGLVGYLGYDMVRQMETLPAKNRDVIGVPEGIMMR
                     PTLFAIFDNVNDELTLVTPAYPAGDVSAEMAWQRAHARLDAAETALERPLPHQPPPVM
                     LPDPPAPSSNFSQQEFVAGVARCKEYIAAGDAFQIVLSQRFSVPFALPPFSLYRALRR
                     INPAPFLFYLDFGGFSIVGSSPEVLVRLRDGIVTVRPLAGTRRRGTSHEEDQALEAEL
                     LADPKELAEHLMLLDLGRNDVGRVSAIGSVKVTESFIVERFSHVMHISSNVEGRLRDG
                     LEALDALIGGFPAGTLSGAPKVRAMEIIEELEPDRRNIYAGCIGYFAANGTMDTCIGL
                     RTALVKDGVMYVQAGAGVVADSVAEAEYEECRQKARALVRAAEEAVRFAAGKAGGNAG
                     G"
     gene            complement(1759..>2623)
                     /locus_tag="VGC82_16895"
     CDS             complement(1759..>2623)
                     /locus_tag="VGC82_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0424999.1"
                     /translation="VVKATKITAGAVTEFDQAKDTLRGRLLAAKAADIMYERANKLDQ
                     LLANGTTLDDLPGDLGVIGVAGTLDSLGNAQTNEPAPIPGAVELKTAIITAAFQARQG
                     DPPQLTEVQTSSAGGSAYYALTVESIIPPGQKPFDAVKEQVADDWTRDQRRRAQDRAA
                     TAMMTSVQAGTSFSDAATAAGVTPRLSPLVTRSQPSSDMAPDLQRVLFGLKKGEATMV
                     ETPEGFVVGQLAEVVKPDAAADKVGYDQARAAITKSISNDVAAVFVDAVRQRAKPQTN
                     QAAFDSVVQPR"
BASE COUNT          457 a          950 c          750 g          466 t
ORIGIN      
        1 gcgtgaaagc gcaccaagcg gatgtgccga tctttctgcg ggatcgtata acctcaatcc
       61 gtgcattgaa gtatgacatc ccgttgcatg attctatact tgtggcggaa ccagccgggc
      121 agcctcaccc cagtaagata atgaagccca cgattccgct gcacatgcta tgggcctttc
      181 atgccgctcg gtaccgtggc atcggaagcg gtttgtcagc ccttagcccc cggcattccc
      241 gcctgccttc ccggcggcga accgcaccgc ttcctccgcc gcccgaacca gcgcccgagc
      301 cttctggcgg cactcctcgt attccgcctc ggcgaccgaa tcggccacca ccccggcgcc
      361 ggcctgaacg tacatcacgc catcctttac cagcgcggtg cgcagtccga tgcaggtatc
      421 catcgttccg ttggccgcga aatagccgat gcatccggcg tagatgttcc gccggtccgg
      481 ctccagttcc tcgatgatct ccatcgcgcg caccttcggc gcccccgaca gcgtgcccgc
      541 cggaaacccg ccgatcaggg cgtccagcgc ctccagcccg tcccgcagcc gaccttcaac
      601 attcgaagaa atatgcatca catggctgaa ccgctcgacg atgaagcttt ccgtcacctt
      661 caccgacccg atcgccgaga cgcgccccac atcgttgcgc cccagatcga gcagcatcag
      721 atgctccgcc agctctttcg gatcggccag cagttccgcc tccagcgcct gatcctcttc
      781 gtgggaggtt ccgcggcgcc tggtccccgc cagcggacgt accgtcacga tgccgtcgcg
      841 cagccgcacc agcacctccg gcgacgaacc cacgatcgag aacccgccga agtccagata
      901 aaacagaaac ggcgccggat tgatccgccg cagcgcccga tacaacgaga atggcggcaa
      961 cgcgaagggg accgagaatc gctgggacag gacaatctgg aaggcgtcgc ccgcggcgat
     1021 gtattccttg cagcgggcca cgccggcaac gaactcctgt tgcgaaaaat tcgaggacgg
     1081 tgccggagga tcgggcagca tcaccggcgg cggctgatgc ggcagcggcc gttccagggc
     1141 cgtctcggcc gcatccaacc gagcatgcgc tcgctgccaa gccatctccg ccgaaacatc
     1201 cccggccgga taagccggag tcaccagcgt cagctcgtcg tttacgttgt cgaaaattgc
     1261 gaacagcgtc ggccgcatca tgatgccttc gggcacaccg atcacgtcgc ggttcttcgc
     1321 cggcagcgtt tccatctgcc gcaccatgtc gtagcccaga tacccgacca gaccgccggc
     1381 catcggcggc agatgtgccg gaacctcgag ttgcgtttcc gcgatcaacg cccgcagact
     1441 gtccagcggc ggcttcgcct ccgccacaaa ggcatgcggc gccgacaacg cgtaccggtt
     1501 caccgaggca cgcccatcct ggcagcgcca caccaggtcc ggctgcatgc cgatgatcga
     1561 gtatcgcccg cgtgccgctc cgccctccac gctttccagc aggaaggagt tcggctggcc
     1621 atgtgccagc ttcaggaaag ccgcgaccgg cgtttccagg tcggctacgg tcttgcgcca
     1681 caccaggctg ccgcgtcccg cttcgtaggc cagccggaaa accgcgaaac ccggggtaac
     1741 cagaacgttc atgtctctct accgtggttg gacgacgcta tcgaacgccg cctgattggt
     1801 ctgaggcttg gcgcgctggc gcaccgcatc gacgaacacc gccgccacat cgttgctgat
     1861 cgacttggtg atcgccgccc gagcctgatc gtagccaacc ttgtccgcgg ccgcatccgg
     1921 tttcaccacc tcggccagtt gtccgaccac aaaaccctcg ggcgtttcca ccattgtggc
     1981 ctcgcccttc tttaggccga acagtacccg ctgcaggtcc ggcgccatgt ccgaactagg
     2041 ctggctccgg gtcaccaggg ggctcagccg cggcgtcacg cctgccgccg tcgcggcgtc
     2101 ggagaacgaa gttcccgcct gaaccgaggt catcatcgcc gtcgccgccc ggtcctgggc
     2161 cctgcgccgc tggtctcggg tccagtcgtc ggccacttgc tccttcaccg cgtcgaacgg
     2221 tttttgcccg ggcggtataa tgctctcgac cgtcagagcg taatacgccg atccgccggc
     2281 ggaagaagtc tgcacctcgg tcaattgcgg cggatcgccc tgtcgggcct ggaaggccgc
     2341 cgtgataatg gcggtcttca gttccaccgc tccggggatt ggcgcaggct cattcgtctg
     2401 ggcgtttccc agcgagtcca aggtgcccgc aaccccgatg acgcccagat cgcccggcag
     2461 atcgtccagc gtggtgccgt ttgccagcaa ctggtccagc ttgttcgcgc gctcgtacat
     2521 aatgtcggcc gccttcgcgg ccagcaaccg cccccgcagc gtgtccttgg cctgatcgaa
     2581 ttccgtaacc gcacccgccg tgattttcgt ggccttcacc acg
//
LOCUS       DASWQN010000989         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1283069, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000989 DASWQN010000000
VERSION     DASWQN010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283069"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1210)
                     /locus_tag="VGC82_16900"
     CDS             complement(200..1210)
                     /locus_tag="VGC82_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0425000.1"
                     /translation="MTEHIETDVAIVGAGPVGLFAVFQLGMLKLSSVLIDALAEVGGQ
                     CTALYPEKPIYDIPAHKAIEAGALITRLEDQIAPFAVPRLLGRQVSGVSGARGAFTLT
                     TDQGDTVRAKAVIVAAGAGAFGPNRPPLDGLAAYEASGAVQYYVRRREDLRGKRVVIA
                     GGGDSAVDWALALKDIAHVAVVHRRAKFRAAPESTARLDEAVRAGEIEMVVPYQLHAL
                     AGSDGILSAVEVATLDGAVRQLPADVLLPFFGLSMDLGPIAAWGLQSEKHHVTIDPAT
                     CQTSLPGVFAIGDVVAYPGKLKLILQGFSEAAVAAHAIFPIVHPDQALHFEYSTTKGV
                     PA"
     gene            1333..1491
                     /locus_tag="VGC82_16905"
     CDS             1333..1491
                     /locus_tag="VGC82_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425001.1"
                     /translation="MTLRVRGAPCRRRHPTLNEADWAGRCLTALNQPIKVPDSVLLML
                     SNRTNGIA"
BASE COUNT          328 a          612 c          595 g          342 t
ORIGIN      
        1 catggccatg acacagcgga ccgtggctgc gacacttgga ccatagcttc gatcagcgcg
       61 ttctggccga agccacgatt cacagaccag ggccgcgata catcgaccat ggccaccaca
      121 catagatcgc ggccatagcg tgttgacgtc aggcgcgaac tggcttgccg cttttttcgc
      181 cgggctgagg gcgctctggc taggccggca cgcctttcgt ggtggaatac tcgaaatgca
      241 gcgcctgatc cgggtggacg atcgggaaaa tcgcgtgcgc cgcgacggcg gcctcgctga
      301 atccttgcag gatcagcttt aacttgccgg ggtaggccac cacgtcgccg atcgcgaaca
      361 cgcccggcag gctggtctgg caagtcgccg ggtcgatggt cacatgatgt ttctcgcttt
      421 gcagccccca ggcggcaatc ggacccagat ccatcgacag gccgaagaac ggcagcagca
      481 cgtcggccgg aagttgcctt accgcgccat ccagcgtcgc gacctccacc gccgacaaaa
      541 tgccatcgct gccggccagg gcgtgaagct ggtacgggac caccatctcg atctcaccag
      601 cgcggacggc ttcatcgagc cgcgcggtgc tttcgggggc ggcacggaat ttcgcccgcc
      661 gatgcaccac ggcgacgtgg gcgatgtcct tcaaagccag cgcccagtcc acggcggaat
      721 cgccgccacc ggcgatcacc acgcgcttgc cgcgcaggtc ctcgcggcgg cgcacatagt
      781 actgcaccgc gccgctggcc tcataagccg caagcccatc caacggcggg cgatttggcc
      841 cgaacgcgcc cgcaccggcg gcaacgatca ccgccttggc ccgcacggta tcgccctgat
      901 cggtcgtcag ggtgaacgcg ccgcgcgcgc cgctcacccc gcttacctgg cggccgagca
      961 ggcgcgggac ggcgaatggg gcgatctggt cctccaggcg ggtgatcaag gcgccggcct
     1021 cgatcgcctt gtgggcgggg atgtcgtaga tcggcttttc gggatacagc gcggtgcact
     1081 ggccgccgac ttccgccagg gcatcgatca gaacgctgga cagtttgagc atgccaagct
     1141 ggaaaacggc gaacaggccg accgggccag cgcccacgat cgccacgtcg gtttcgatgt
     1201 gttctgtcat agcgccctca tactttgctt ttccgccagc acaagcgttc gtaacccctc
     1261 ggtgaaggct tctcgcgctt gcccgactat ggtccaaccg tctcccagtg ttttttatgt
     1321 cgggaaattc gcatgacgtt gcgcgttcgt ggcgcaccct gtcgtcgtcg ccatcccacc
     1381 ttgaacgagg cagactgggc cggacgctgc ctgacggcgt tgaaccagcc gatcaaggtc
     1441 ccggattccg tcctgctgat gttgagcaac cgcacgaacg ggatcgcttg aaccatggcg
     1501 ccgggctgcg gtatcggctg gatgtcgcca gccgggatct gccgttgccg tggaacgctt
     1561 cgtccacccg acgatcgggt cgcttcgcac ccgacggcag cagcggtggg agaggtatcg
     1621 ctggatgttc tggaagtcga tgactgaccg tgggatggct gcgtcaggcc caccggaaca
     1681 gggcgttggc gctgttgcct gtgcctggat tcatccaaga ggaagcgccg cctgcaaaag
     1741 cattttgttt catcgcacga ttgacacgct tcggacctcc cgcccagcgt gatcctccag
     1801 ggtgatttga agtataaccc cgggaattgg tgcgggtggc gagtgggctc cgtcgtcctt
     1861 atttgacacg caggcag
//
LOCUS       DASWQN010000990         5722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_887125, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000990 DASWQN010000000
VERSION     DASWQN010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887125"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VGC82_16910"
     CDS             <1..270
                     /locus_tag="VGC82_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0425002.1"
                     /translation="TPITLRLIQDGASHLVLTGPMPIHCPVRLLHGQADPDVPWELAL
                     LIAERVVTPDVHVTLVKHGDHRLSRPADLMLLTRTIAALLGQDGA"
     gene            complement(226..1071)
                     /locus_tag="VGC82_16915"
     CDS             complement(226..1071)
                     /locus_tag="VGC82_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0425003.1"
                     /translation="MNQLLVFGLGYTGSAIADAARSAGFLVAGTTRAGLDQSLCFDQA
                     EHAIQSATHLLTTAAPDQDGDPVLANYAGAIAAAPDLRWIGYLSSTVVYGNRDGQWVD
                     EDTAVAPSQARGQRRVEAEDAWSRFAEKAGVDIFRLAGIYGPGRSTFNDLRAGRARRM
                     KKAGHMFGRIHRDDIARAVVAAMRQERTAGRRVLNLADDEPSESAAVVVEAASLLGMT
                     APAEVAFEDALSAMTPMARSFWAENRKVSNAKTKAALGIEWLYPTYREGLRAILAQEC
                     GDRPG"
     gene            complement(1068..1817)
                     /locus_tag="VGC82_16920"
     CDS             complement(1068..1817)
                     /locus_tag="VGC82_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0425004.1"
                     /translation="MKWAPALFGLLALSLWEAAVRIAEVPVYQLPGPIAIIGAFVADP
                     LGLLQALASTLIVTLAALLVAAVLGVAMAVAMSASRLAQAAIGPWAVALQVTPLPAIA
                     PLIIIWVGDPFVALVVCATIVAFFPLFANTATGLASAPSELSDLFRLYGASRWRTMTL
                     LRLPAALPYFLTGLRISGGLALVGAVVAELVAGSGGFASGLAYRILEASFRLEMPRMF
                     AALVLLAVAGIVLNFGLGALGQAILRRRGLA"
     gene            complement(1814..2548)
                     /locus_tag="VGC82_16925"
     CDS             complement(1814..2548)
                     /locus_tag="VGC82_16925"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007283738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425005.1"
                     /translation="MLTVSNVGRRFPGGIEALRDASLQLSEADFVVLLGPSGCGKSTL
                     LRLIAGLDQPDSGRLAWDSGPPATGDIGYVFQDATLLPWATAEDNVFLPLRLRGVRRE
                     AAMPDVRAALDHVGLRGFETARPGQLSGGMRMRVSIARAMVTRPRLLLMDEPFAALDE
                     FTRHKLQADLLEVWRQAGCTVVFVTHSIYEAAFLARRVVLMTPRPGRIAAEIASGLVS
                     RPDTRLDPAYAALVAEIARSMEDALR"
     gene            complement(2556..3542)
                     /locus_tag="VGC82_16930"
     CDS             complement(2556..3542)
                     /locus_tag="VGC82_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0425006.1"
                     /translation="MRWLTAILGLLLLSGAARADDKITFGLDWKAEAEYGGYYQAVAT
                     GIFAKHGLDVTIQQGGPQVNHTQLLLAGRLDFNISSNSFLALNFVKENIPFRAVAGMF
                     QKDPVVLIAHPGQGNDSFPALKGKPIMISGDSRVGWWTFLKAKFGYTDSQIRPYTFNP
                     APFLADKTAVQQGYLGSEPFSIKEQAHFEPVVLLMADAGFKGYASMIATSDKKIEQQP
                     DLVKRFVNASIEGWYSYLYGNPTPGNALIKQANPEMNDALIGYGIDAMKRYGILDSGD
                     ALTKGIGTMSDARWAEFYQTVSEQGVYPKGLDVTKAYTLRFVNQQVGMDLKH"
     gene            3561..4337
                     /locus_tag="VGC82_16935"
     CDS             3561..4337
                     /locus_tag="VGC82_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0425007.1"
                     /translation="MIAGENGTDTPGGKMGTELSYGLAGRRAIVTGHRGGIGGAIRDT
                     LMHDGVEVIGLDLPDFDLSDIATLEPRLAAIIAECGPVDILVNNAGVTVLGTVLETSL
                     AETEKVFRVNFLAAYAVMRAVLPGMVERRRGAIVNIASDQALIGKQVSAAYGASKAAI
                     AQLSRSAALDWARFGIRVNCIAPGSTQTAMLGTVIDQLSVRYPDQFPVSSTDAYKAGV
                     PLGRFAEPSEIAAAVAFLASDAASFITGIVLPVDGGGTAQ"
     gene            4334..5023
                     /locus_tag="VGC82_16940"
     CDS             4334..5023
                     /locus_tag="VGC82_16940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0425008.1"
                     /translation="MKLAVVTGGTRGIGRAVADRLATEGWDVLVLARHDATSSHRFIP
                     CDVADAQAVRDVFSGLDRVDALVNCAGIAGANRLDGDDVLWHAIVGSNLHGTYHCCKA
                     ALTLLPDGTGRIVNIASVLGLRGVPDQTAYCAAKHGVVGFTRALALALAPRGITVNAL
                     CPGWVDTDMAAGRYVELGITAEQAAAGVPTGRIASPAEVADAAVWLLRPEARGITGHA
                     LPIDGGGLALP"
     gene            5090..>5722
                     /locus_tag="VGC82_16945"
     CDS             5090..>5722
                     /locus_tag="VGC82_16945"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEY0425009.1"
                     /translation="MVTLGSGDYQYEPVEDWGKLPSGWSFKEIGGVGVDDRDNVYVFN
                     RGEHPMIVFDRDGNFLRAWGEGLFPRAHGVFVAPDQTLWLTDDGDHTVRQCTPEGKVL
                     LTLGTSGKPAPYMSGEPFHRCTHTAMAPNGDIYVSDGYGNARVHKYAPDGRLLLSWGG
                     PGTDPGEFNIAHNITCDADGWVYVADRENHRVQVFDGNGRYETQWNNLHRP"
BASE COUNT         1037 a         1887 c         1837 g          961 t
ORIGIN      
        1 acccccatca ctttgcgatt gatccaggat ggggccagcc atctggtgct caccggcccg
       61 atgccgatcc attgcccggt ccggttgctg catgggcagg cggacccgga cgtgccgtgg
      121 gaactggctt tgcttatagc cgaacgggtc gtcacgccgg atgtccatgt cacgctggtg
      181 aagcatggcg accaccgctt gtcgcgacct gcggacctta tgcttttaac ccggacgatc
      241 gccgcactcc tgggccagga tggcgcgtag gccttcgcgg taggtcggat acagccattc
      301 gatccccaat gctgccttcg tcttcgcgtt gctgaccttg cgattctcgg cccagaagct
      361 gcgcgccatc ggggtcatgg cggacagagc gtcctcgaag gcaacctcgg ccggagcggt
      421 cattcccagc agcgaagcgg cctccaccac cacggccgca ctttccgaag gttcgtcgtc
      481 agccagattc aacacccggc gtccggctgt gcgctcttgc cgcatggcag cgaccacggc
      541 tcgggcaatg tcgtcacgat ggatgcgtcc gaacatgtgc ccggcctttt tcatccgccg
      601 cgcccgtccc gcccgcaggt cgttgaatgt ggatcgcccc ggcccgtaaa ttcccgccag
      661 gcggaaaatg tccacgccgg ctttttcggc aaatcgtgac caggcgtctt ctgcctccac
      721 ccgccgttgc ccgcgcgcct gcgacggggc gacggcggtg tcttcatcga cccattgtcc
      781 atcgcggttg ccatagacca ccgtgctgga caggtagccg atccagcgca gatcgggcgc
      841 cgcggcgatg gcgcccgcgt aatttgccag caccgggtcg ccatcctggt cgggcgcggc
      901 tgtcgtcagc agatgcgtcg cggattggat cgcgtgttcg gcctgatcga agcacaggct
      961 ttggtccaat ccagcccgag tcgtgccggc aaccaggaag ccagcagagc gtgccgcatc
     1021 ggcgatggcc gaaccggtat agcccagccc aaataccaac aactggttca tgccagacct
     1081 cgtcgccgca aaatcgcctg acccagcgcg cccaggccga aattcagcac gataccggca
     1141 acggccagca gcaccagggc agcgaacatg cgcggcatct ccagccgaaa gctggcctcc
     1201 aggatacggt aagccagtcc cgaggcaaaa ccgccggagc cggcgaccag ttctgccacc
     1261 accgcaccaa ccagcgccaa gccgccggaa atgcgcaagc cagtcaagaa atagggcagc
     1321 gcggccggca accgcaacaa cgtcatggtt cgccaacggc ttgcaccata cagccggaac
     1381 aggtcgctca gctccgaggg cgcggaggcc agtcccgtcg cggtgttggc gaacaacggg
     1441 aaaaacgcga cgatggtggc acagaccacc agggcgacga acggatcgcc gacccaaatg
     1501 atgatcagcg gcgcaatcgc cggcagcggc gtgacctgca gcgccaccgc ccaaggcccg
     1561 atcgcagcct gagccaagcg gctggccgac atcgccaccg ccatggccac tccaagcacc
     1621 gccgcgacga gcagcgccgc aagggtgacg atcaaggttg aggccagcgc ctgcaacaac
     1681 cccaacgggt cggccacgaa ggcgccgata atcgcgatcg gtccgggcag ttgataaacc
     1741 ggaacctccg cgatccttac cgctgcctcc caaagactca gcgccagcag gccgaacaag
     1801 gcgggcgccc atttcaccgc aacgcgtctt ccatgctgcg ggcaatctcc gccaccaggg
     1861 cggcgtaggc cgggtccagg cgagtatccg gccgggatac cagaccggac gcgatctccg
     1921 cggcgatgcg gcccggacgg ggcgtcatca gcaccacgcg gcgggccagg aaggcagcct
     1981 catagatgga gtgggtaacg aacaccaccg tgcagccggc ctggcgccag acctccagca
     2041 gatcggcctg cagcttgtgc cgggtaaatt cgtccagtgc cgcgaatggc tcgtccatca
     2101 gcaacaggcg cggccgggtg accatggcgc gggcgatcga cacccgcatc cgcatgccgc
     2161 ccgaaagctg tcccggccga gcggtctcga acccgcgcag gcccacatga tccagggcgg
     2221 cgcggacatc cggcatcgcg gcctcacgcc ttacgccgcg caggcgcagc ggcagaaaca
     2281 cattgtcctc ggctgtagcc cagggcagca gggtggcatc ctggaacacg tagccgatgt
     2341 cgccggtcgc cggcggaccg gagtcccacg ccaatcggcc gctgtccggc tgatccaacc
     2401 ccgcgatcag ccgcagcaac gtcgatttgc cgcagccgga gggccccagc aacacgacga
     2461 aatcagcttc ggacagttgc aaagaggcgt cccgcaaggc ctcgattccg ccgggaaagc
     2521 ggcggccgac attgctgacc gtcagcacag cagggtcaat gcttcaggtc catcccgacc
     2581 tgctggttca cgaaccgcag cgtgtacgcc ttggtaacgt cgagcccctt gggatacacg
     2641 ccctgttctg agacggtctg atagaactcg gcccaacgag catccgacat ggtgccaatt
     2701 cctttcgtca gcgcgtcgcc cgagtccagg atcccgtagc gcttcatcgc gtcgatccca
     2761 tagccgatca aagcgtcgtt catttccgga ttggcttgct tgatcagcgc gttgcctggc
     2821 gtcggattgc catacaggta ggagtaccaa ccctcgatcg aggcgttgac gaaccgcttc
     2881 accaggtccg gctgctgctc gatcttcttg tccgaggtcg cgatcatgct ggcatagccc
     2941 ttgaagccgg catcggccat cagcagcacg acgggctcga aatgcgcctg ttccttgatc
     3001 gaaaacggct ccgacccgag ataaccctgc tgcactgcgg ttttatccgc caggaacggc
     3061 gccgggttga aggtgtaggg acggatctgg ctgtcggtgt agccgaactt cgccttcagg
     3121 aatgtccacc agccgacccg agagtccccg ctgatcatga tcggcttgcc tttcagcgcc
     3181 gggaaactgt cattgccctg ccctggatgg gcgatcagca ccaccggatc cttctggaac
     3241 atgccggcaa ccgctcggaa cgggatgttt tccttgacga aattcagcgc caggaaggaa
     3301 ttggacgaga tattgaaatc gagccgcccg gcgagcagca attgcgtgtg gttgacctgc
     3361 ggtccgcctt gctggatcgt cacgtccagc ccgtgcttcg caaaaatgcc ggtggcaacg
     3421 gcctggtagt agccgccata ttccgcctcg gccttccagt ccaaaccgaa ggtgatctta
     3481 tcgtccgcgc gcgcggcacc ggacagcagc aacagaccca gtatagcggt cagccaacgc
     3541 atcgactgga ttccctgccc gtgatcgctg gcgagaatgg caccgatacg cctggaggca
     3601 agatgggtac ggaattgagc tacggcctgg ccggtcggcg ggcgatcgtt accgggcatc
     3661 gcggcggcat cggcggcgcc atccgcgata cgctgatgca tgacggcgtc gaggtcatcg
     3721 gtctcgacct gccggatttc gacctgtccg atatcgcgac gctcgagcca cgactcgccg
     3781 cgatcatcgc cgaatgcggg ccggtagata tcctggtgaa caacgccggg gtcactgttc
     3841 tgggcaccgt gctggaaacg tcgctcgcgg agaccgaaaa ggtattccgg gtcaatttcc
     3901 tagccgccta tgcggtgatg cgcgcggttc tgccgggaat ggtggagcgg cggcggggcg
     3961 cgatcgtgaa catcgcatcg gaccaggcgc tgatcggcaa gcaggtcagc gccgcctatg
     4021 gcgcgtcaaa ggcggcgatc gcccagcttt cccgatcggc cgcattggat tgggcgcggt
     4081 tcggcatccg ggtgaactgc atcgcccccg gttccacgca aaccgcgatg ctgggcactg
     4141 tgatcgacca gctctcggtg cggtatcccg accagtttcc ggtgtcgtca accgatgcct
     4201 acaaggccgg ggtgcctttg gggcgttttg ccgaaccatc cgagatcgcc gcggcggtgg
     4261 cgtttttggc ttccgacgcc gcatcgttca tcaccgggat cgtgctgccg gtggacggcg
     4321 gcgggacggc gcaatgaagc ttgccgtcgt taccggagga acgcgcggga tcggccgggc
     4381 ggtggcggac cgcctggcta ccgagggttg ggacgtgctg gttctggctc ggcatgacgc
     4441 gacgtcctcg catcgcttta tcccgtgtga cgtggctgat gcccaagccg tgcgtgacgt
     4501 gttctcgggg ctggaccggg tcgatgcgct ggtgaactgt gccggcatcg cgggcgccaa
     4561 tcggctggat ggcgacgacg tcttgtggca cgcgatcgtc gggtcgaatc ttcacggcac
     4621 ctatcattgc tgcaaggcgg cgctgacgtt gctgccggat gggactggcc ggatcgtcaa
     4681 catcgcctcg gtcctggggt tgcgtggcgt gcccgaccag accgcatact gcgccgccaa
     4741 gcacggcgtc gtcgggttta cgcgggcgct ggcgctggcg ctcgcccccc gcgggattac
     4801 cgtcaatgcg ttgtgtcccg gctgggtgga taccgacatg gcggctgggc gctatgtgga
     4861 gcttggtata acggcggagc aggcagcggc gggggtgccg acgggtcgga ttgcctcacc
     4921 ggcggaagtc gccgatgcgg cggtctggct gctgcggccc gaggcgcgcg gcattaccgg
     4981 tcacgcgttg cccatcgatg gcggtgggct ggcgctgccg tagcgcccct ggccgaaccg
     5041 cccgacccat gacaagctgc tcccaatcga acaagcaggg gaaacgcaga tggtcacctt
     5101 gggcagcggc gactaccagt acgagccggt tgaagactgg ggaaaactcc catctgggtg
     5161 gtcgttcaag gagatcggcg gcgtcggcgt ggacgacagg gacaatgttt acgtcttcaa
     5221 ccgcggcgag cacccgatga tcgtgttcga ccgcgacggc aacttcctgc gcgcctgggg
     5281 cgagggcctg ttcccgcgcg cccacggcgt cttcgtcgcg ccggaccaaa ccttgtggct
     5341 gaccgacgat ggcgaccaca ccgttcgcca atgcacgccg gagggcaagg tcctgctgac
     5401 gctgggcacc tccggcaagc cggcgccgta catgagcggc gagccgttcc atcgctgcac
     5461 ccacaccgcg atggctccca acggcgacat ctatgtgtcc gacgggtacg gcaatgcccg
     5521 ggtccataaa tacgcgccgg acggccgcct gctgctgtcc tggggtggac ccggcacgga
     5581 tcccggggag ttcaacatcg cgcacaacat cacctgcgac gccgatggct gggtgtatgt
     5641 cgccgaccgg gaaaatcatc gcgttcaggt gttcgacggc aacggccggt acgagaccca
     5701 gtggaacaac ctgcatcgtc cc
//
LOCUS       DASWQN010000991         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1283248, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000991 DASWQN010000000
VERSION     DASWQN010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283248"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2277)
                     /locus_tag="VGC82_16950"
     CDS             complement(<1..2277)
                     /locus_tag="VGC82_16950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0425010.1"
                     /translation="MPTLSEAPAESREATLAKSFEAFFDSDWYQAQYPDIAAANLDPI
                     IHFIRHGLPERRDPNRFFDGAWYTEHYPDVGAGGMHPLVHYLQAGAAELRNPHPNFDA
                     VYYTDQHPEAAANPLLYHIRVGQARGYLTEKPIRIQDYLPSTQQAPRWSGKIVVDVVI
                     PVYRGLEETQRCIGSVLADPAKPLGRVIVVDDRSPEPELVNWLQDLAAAKRIVLVRNP
                     SNMGFVASVNRGMEEAENRDVVLLNSDTEVPRGWLGRLTAQAYADTNIATVSPLSNNA
                     TICGYPDNAGGPIVFRQTVSELDDICRTVNAGRWVDAPTTVGFCMYIRRKALREVGDF
                     DAERFQVGYGEENDFCLRASALGWRHRIACDTFVYHKGSVSFGDRAAKLAARAMKLIL
                     ERYPSYQRDIARHVSLDAIAPFRFAVTAALFHRSKLPVILVIAHELGGGVRRHIDMLI
                     ERFRDQAHFLLLEATDRGAALSIPCLPDHPSLALPPERLDDLILLLQSTRVSRIHIHH
                     LLGMDMDIRSLVRRLDLPLDVTVHDYYAICPQINLLPWRHSLYCGEPDIAGCNACIAH
                     RSSHGARDILTWRSERDWQFREAARVLCPSADVLARLKRHGLASNAVLAPHEPVEASP
                     WPLHVRSPGEGKLRIAILGTLVDHKGGRTVASVAEMADPKTMEIHLIGHTDGQFSRRA
                     LKRMKITGKYNEADLPGLIEAVSPHLIWFPAVWPETFSYTLSAAIDAGIPVAATAIGA
                     HTERLRGRPCTWLADIATS"
BASE COUNT          484 a          783 c          904 g          492 t
ORIGIN      
        1 ggaggtggcg atgtcggcaa gccaggtgca cgggcggcct cgcaagcgtt cggtatgggc
       61 gccgatcgcc gtggctgcga ccgggatgcc ggcgtcgatc gccgcactca gcgtatagct
      121 gaacgtttcc ggccagacgg cgggaaacca gatcaaatgc ggcgagaccg cttcgatcag
      181 cccgggcaaa tcggcctcat tgtatttgcc ggtgatcttc atccgcttca gtgcccggcg
      241 ggagaactgg ccgtcggtgt ggccgatcag gtgaatctcc atcgtcttcg ggtcggccat
      301 ttcggcgacc gaggcgacgg ttcgtccgcc cttgtggtca accaacgtgc cgaggatggc
      361 gatccgcagc ttgccctcgc caggcgatct gacgtgcaac ggccagggag aggcctcgac
      421 cggttcgtgc ggcgcgagca cggcattgga tgccaggcca tgccgcttca ggcgcgccaa
      481 aacgtcggcg ctggggcata gcacgcgggc agcctcccgg aactgccagt cacgctccga
      541 ccgccacgtg aggatgtcgc gggcgccgtg tgagcttcga tgcgcgatgc aggcgttgca
      601 gccggcgatg tccggttcgc cacagtaaag actgtgccgc cacggcagca ggttgatctg
      661 tgggcagatc gcgtaatagt catgcacggt gacgtcgagc ggcagatcca ggcgacggac
      721 cagggaccgg atgtccatgt ccataccaag aagatgatga atgtggatac gcgaaacccg
      781 cgtcgattgc agcaacagga tcaggtcgtc caggcgttcg gggggcaagg ccagggaagg
      841 atggtctgga agacatggga tcgaaagggc ggcgccccgg tccgttgcct ccagcagcag
      901 gaaatgggct tggtcccgga agcgctcgat cagcatgtcg atgtgccggc gcacgccgcc
      961 gcccaattcg tgcgcgatca ccaggatgac aggcaatttc gatcggtgga acagcgccgc
     1021 cgtgacggcg aaccggaatg gcgcgatggc atcgaggctg acgtggcgcg cgatgtcgcg
     1081 ttgatagctg ggatagcgct ccaggatcag cttcatggca cgggcagcca gtttcgccgc
     1141 gcggtcgccg aagctgacgg agcccttgtg atagacgaaa gtatcgcagg cgatccggtg
     1201 gcgccaaccg agggcgctgg cgcgcaggca gaaatcgttc tcctcgccgt atcccacctg
     1261 gaagcgttcg gcatcgaagt cgccgacttc ccgcagagcc ttgcgacgga tgtacatgca
     1321 gaacccgacc gtggtggggg cgtccaccca gcggccggca ttgacggtgc ggcagatatc
     1381 atcgagttcg gacactgttt gccggaacac gatcgggccg ccggcattat ccggatagcc
     1441 acagatcgtc gcgttgttgg acaacggaga gaccgtcgcg atgttggtat cggcgtaagc
     1501 ctgggcggtg agccggccca gccagccccg gggaacctcg gtatcgctgt tcagcagcac
     1561 tacgtcgcga ttctcggctt cttccatgcc ccggttgacc gacgcgacga atcccatatt
     1621 gctggggttg cgcaccagaa cgatgcgttt tgcagcggcg agatcctgaa gccagttgac
     1681 gagttcgggt tccggggacc ggtcatcgac aacgatcacc cggccgagcg gtttggcggg
     1741 atcggccagg accgatccga tgcaccgctg ggtttcctcc aggccgcgat agaccggtat
     1801 caccacatcg accacgatct tgcccgacca tcgcggtgcc tgttgcgtcg atggcaggta
     1861 gtcctggatc cggatcggct tctcggtcag gtagcctcgc gcctgaccga cccggatgtg
     1921 gtatagcagg ggattagccg ccgcctcggg atgctgatcg gtgtagtaga cggcgtcgaa
     1981 gttgggatgc ggattgcgga gttccgcagc acctgcctga agataatgca ccaggggatg
     2041 catcccgccg gcgccgacgt ccgggtaatg ttcggtgtac caggcgccgt cgaaaaaccg
     2101 gtttgggtca cgccgttccg gcaggccatg ccggatgaaa tggatgatcg gatcgaggtt
     2161 tgccgcggcg atgtcggggt attgcgcctg gtaccaatcg gagtcgaaaa acgcctcgaa
     2221 ggacttcgca agcgtggctt cgcggctttc cgccggtgct tcggaaagcg tcggcatttt
     2281 gttcggcttg gccatgcgtt agagcgcttt cgggctggct gggaaaggtg cgaacccgtt
     2341 tgccgcctgg cgtcgaaaag tcgctgccgt gcttggcgcg gtcaccgacg gccttgaagt
     2401 tgacctgcta gggcaggcca acggccgcta gcagcctgtc ggagttggtt tttggggtat
     2461 atttgatggg tgaggtcgct tgtgcctgca tgacggcctc tgacgcgtgg ccgcaaagcg
     2521 gaattcacgc ttgagacctc ggccatcgcg ccaacgagcg ttgtgcccct cgcaaatcca
     2581 ccccagtcga cgcaaaaagg tcgaattgag gctcagttcg ccccggccag ggcgaacatc
     2641 cgcttcatgt tccacgccat ggt
//
LOCUS       DASWQN010000992         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2603220, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000992 DASWQN010000000
VERSION     DASWQN010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2603220"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VGC82_16955"
     CDS             complement(<1..483)
                     /locus_tag="VGC82_16955"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY0425011.1"
                     /translation="MAAPRPVLRGRLAEQLWSRRPEEQARASLRQEIHRLLEALSPAE
                     ADILIITRDHLTLRPGAVWVDVEDVMRATASQPASLALLDGELLEDLNGIDPSFDAWL
                     QTAREKLRDQARGIAEAMLREELKPDAAIAAAQRLLAIDRCHEGAWRSLMKAHSDKGE
                     T"
     gene            complement(724..1362)
                     /gene="cmk"
                     /locus_tag="VGC82_16960"
     CDS             complement(724..1362)
                     /gene="cmk"
                     /locus_tag="VGC82_16960"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY0425012.1"
                     /translation="MTMKPVIAIDGPAAAGKGTLARRLANTLGLPYLDTGLLYRAVGR
                     RVLDTGGDPRDPAAAERAARSLQPADLQRVDLRGPQADAGAASVASIPAVRTALLAFQ
                     HDFAVRHGAVLDGRDIGTVIFPHATAKLFVTASLAERARRRWLELQAKGMEADIATVE
                     QDLCQRDAKDAARAAAPLRAADDAYRLDTSAMDAEAAFQAALVFIRTRIDIA"
     gene            complement(1359..2702)
                     /gene="aroA"
                     /locus_tag="VGC82_16965"
     CDS             complement(1359..2702)
                     /gene="aroA"
                     /locus_tag="VGC82_16965"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY0425013.1"
                     /translation="MDAAAPLKLTSCKPPVPLNGRIAVPGDKSISHRALMFGALAIGE
                     TRITGLLTGEDVLRTAAAMRALGAEVLHDPDGIWRVAGRGIGGLTEPADVLDMGNSGT
                     AARLLCGILASHDLFSVMTGDASLRGRPMRRVIDPLTATGARFMAREGGRLPLAIQGA
                     RNAMPLDYRVPVPSAQVKSAVLLAGLNAPGITRVDEPEATRDHTENMLRHFGATVGVD
                     VSGPGRTIELQGQPELRAADVVVPGDPSSAAFPLVAALLVPGSKITIPGVGLNALRTG
                     LFRCLEEMGADIAIRNRRTEGGEPVGDLDVTAGPLRAVDVPAERAPSMIDEYPILAVA
                     AASAAGTSRLRGLKELRVKESDRLAATAALLSINGVRVEIEGDDMIVHGTGTPPPGGG
                     LVQTHMDHRIAMSAIVLGLVAGSPVTVDDAGFIDTSFPRFVALMNGIGAAIRPAA"
     gene            2981..3313
                     /locus_tag="VGC82_16970"
     CDS             2981..3313
                     /locus_tag="VGC82_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEY0425014.1"
                     /translation="MAKPELGIKRVCVACSTRFYDLTKSPAVCPKCGTEQPIEQPRPR
                     RTGGNVVEDKRPKKVAAAPEDADVEVEGVEDVEEEDVLESTDDLEDDTDAISTDIEVE
                     NDTDEHER"
BASE COUNT          553 a         1089 c         1126 g          546 t
ORIGIN      
        1 tgtctcgccc ttgtccgagt gcgccttcat caacgaccgc caagcccctt cgtgacagcg
       61 atcgatagcc agcaaacgct gcgctgcggc gatagccgca tccggcttca gctcttctcg
      121 caacattgcc tcggcgatac cgcgagcctg atcgcgcagc ttttcccgcg ccgtttgcag
      181 ccaggcatcg aagctcgggt cgatcccgtt caggtcctcc agcaactcgc catccagcag
      241 tgccaacgag gctggttgcg aggccgttgc ccgcatcacg tcctcgacat cgacccacac
      301 cgcccccggt cgtaacgtca ggtggtcgcg ggtgatgatc aggatgtcgg cttccgccgg
      361 tgaaagcgct tccagcaggc ggtgaatttc ttgccgcagc gatgcccggg cctgctcctc
      421 cggccggcgg ctccagagtt gttccgcaag gcggccccgc agtaccgggc gcggcgcagc
      481 catcgcaatg ctcgccagca acgcccgggt tttcgcccag atggcagcac gctctgacct
      541 tgggtggtgg acgcttgcat ctggccgatc agtcgcaggc gcacgacgac acggtccagc
      601 gcgtgcggtg tctgcgttcg tgacctggta gtagaggccc tcatcacgct tccgaaggaa
      661 cggttgtcga gcctgtggtc tcccgacgcg actgaaatgg ctttggcgag gggccgcaga
      721 gtctcaagcg atgtcgatcc gtgtgcgaat gaaaaccaga gctgcctgga acgcggcctc
      781 ggcatccatt gcgctggtgt ccagcctgta tgcatcgtcg gccgcccgca gcggcgccgc
      841 tgcccgggcc gcgtccttgg catcgcgttg gcaaaggtcc tgttcgaccg tggcaatgtc
      901 ggcttccatg cccttggctt gcagctccag ccatcggcgc cgggcgcgct ccgccaggct
      961 cgcggtcacg aacagcttgg cggtggcatg ggggaaaatg acggtaccaa tgtcgcggcc
     1021 atccaggacc gcgccgtggc gaacggcaaa atcgtgctga aacgccagca gcgccgttcg
     1081 cacggcagga atggaggcga ccgaggcagc ccccgcatcc gcctgaggcc cacgcagatc
     1141 gacacgctga agatccgccg gctgcaacga cctggccgcg cgttcggctg ccgccggatc
     1201 gcgggggtca ccgccagtgt ccagcacacg ccggcccacg gcccgataca gcagaccggt
     1261 gtccaggtac ggcaaaccga gcgtgttggc gagacggcgg gcgagggtgc cttttccggc
     1321 cgcggccggg ccgtcgatgg cgatgactgg cttcattgtc atgccgccgg acggatggcg
     1381 gcgccgatgc cgttcatcaa ggcgacgaag cggggaaagc tagtgtcgat gaagccggca
     1441 tcgtccacgg tgactggcga cccagcgacc aatccgagga cgatcgccga catcgcgatg
     1501 cgatgatcca tatgggtttg caccaggccg ccgccggggg gcggcgtgcc ggtgccgtgg
     1561 acgatcatgt cgtcgccctc gatttcgacc ctgacgccgt tgatcgagag cagcgcggcg
     1621 gtcgcggcga ggcggtcgct ttccttgacg cgcaattctt tcaggccgcg caggcggctg
     1681 gtaccggcgg cgctggcggc ggcaacggct aggatcggat attcgtcgat catgctgggc
     1741 gcgcgttcgg ccggtacatc gaccgcccgc agcgggccgg cggtgacgtc cagatcgccg
     1801 actggctcgc cgccctcggt ccggcggttt cggatggcga tgtcagcgcc catctcttcg
     1861 aggcaccgga acaggccggt gcgcagcgca ttcagcccaa cgccggggat cgtgatcttg
     1921 ctaccgggga ccagtaacgc agcaaccagc gggaacgcgg ccgaggacgg atcgcccggc
     1981 accaccacgt ccgcggcccg cagctccggc tggccttgca actcgatggt ccggccaggg
     2041 ccgctgacat ccacgccgac ggtggcgccg aaatggcgca gcatgttttc ggtatggtcg
     2101 cgcgtggctt ccggctcatc gactcgggtg atgccggggg cgtttagacc agccagcagc
     2161 actgccgact ttacctgcgc cgagggaacc ggcacccggt aatccagcgg catcgcgttg
     2221 cgggcgccct ggatcgccag cggcagccgg ccaccctcac gcgccatgaa cctcgcgccc
     2281 gtggcggtca atgggtcgat cacccggcgc atcgggcggc cgcgcaggct ggcatcaccg
     2341 gtcatcacgg agaacagatc gtggctggcc aggatgccgc acagcaatcg ggcggcggtg
     2401 ccggagttcc ccatgtccag gacgtcggca ggttcggtca gtccgccgat gccacggccg
     2461 gccacacgcc agatgccgtc cgggtcgtgc aagacctcgg cgcccagggc gcgcatagcg
     2521 gctgccgtac gcagcacgtc ctcgccggtc aacaggccgg tgatccgtgt ttcaccgatg
     2581 gccagcgcgc cgaacatcag cgcgcgatgg ctgatcgact tgtcgcccgg gacggcgatc
     2641 cggccgttca gcggcaccgg cggcttgcag gaggtcaact tgagtggagc ggcggcgtcc
     2701 atgatatcgt tccggtgctt gactggtggg cttgggctgg ctggcttgag ctggtagggc
     2761 tggggctggc tggctttgac tggcgggctt ggcgcgccgt gctggataat ggctggatgg
     2821 cttgttaaag actttcgggc gagaccgata ccgctacaaa cggcataagg catttgacag
     2881 ggctgcgccg gggtggcatg ggcgcccgct tccaaccgaa gccccaccac tggttctctt
     2941 tcgggggcgg ctaccctgtc acgaagggcc ttatataccc atggctaagc ctgaactcgg
     3001 catcaagcgg gtctgcgtcg cgtgcagcac gcgattctac gacctgacca agtcgccggc
     3061 cgtctgccca aaatgcggga cggaacagcc gatcgaacaa cctcgtccgc gccgtaccgg
     3121 cggcaacgtg gtcgaggaca agcgcccgaa gaaggtggct gccgcgcccg aggacgccga
     3181 tgtggaagtc gagggtgtcg aggatgtcga ggaagaggac gtcctggagt ccaccgatga
     3241 cctggaggat gacaccgacg ccatcagtac ggacatcgag gtcgaaaacg acaccgacga
     3301 gcacgagcgc tgag
//
LOCUS       DASWQN010000993         4290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1942630, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000993 DASWQN010000000
VERSION     DASWQN010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942630"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VGC82_16975"
     CDS             <1..190
                     /locus_tag="VGC82_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425015.1"
                     /translation="QVQPPQGAADGLTAAAACKPFAHERRQTPQCPARFRIGPGYGWC
                     GCGLPGGADFCAKRCFDL"
     gene            complement(187..444)
                     /locus_tag="VGC82_16980"
     CDS             complement(187..444)
                     /locus_tag="VGC82_16980"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0425016.1"
                     /translation="MLRVDYDRWGQTPADLRHIALNAAHTRTRERMLALHDIAQGACA
                     TQVAERTDRRPQTVMDWVHSYNEPGPEALSYRRTGGRRPFA"
     gene            complement(597..1997)
                     /locus_tag="VGC82_16985"
     CDS             complement(597..1997)
                     /locus_tag="VGC82_16985"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0425017.1"
                     /translation="MRRHSGLMDLVPLAATLSTEGFHWRSPLPPYQKAGIARLLAEPG
                     VLLADEMGLGKTVQAIGALRVLLQRGAQGPALVVVPTGLVLQWRRQFREWAPELTLST
                     CIGGPEERRQRWLAPAQVYLTGFDALRGDMALPAPYGPRRRLWQVVVADEAQRIKNAH
                     TAIAATVKALPRRRAWALTGTPLENCPDDTVSILEFVAPGLCDPAEMVVGLRKALSLV
                     QLRRRRTDVLPELPPKTVFEIAPDLLPVQRAAYDIARREGLVWLRSLGAELRVTHVLE
                     LILRLKQICNACPRTGASAKLADLARRLDIVAQAGEKALVFTQFVAMPFGAEAIAQAL
                     KPLNPLLLTGRMSAAARDDAIALFAASPDRHVLVSSLRVGGVGLNLTAASVVFHFDRW
                     WNPAVETQAEDRVSRIGQTRAVQVFAYLTPDSIEQRIGEILDEKRALFADIIDGLATD
                     GLRRLDLPTLLHAVGA"
     gene            complement(1984..3237)
                     /locus_tag="VGC82_16990"
     CDS             complement(1984..3237)
                     /locus_tag="VGC82_16990"
                     /inference="COORDINATES: protein motif:HMM:NF022376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HEY0425018.1"
                     /translation="MTVEFRMAIEALRAGVPNRAAIRQMGTEQTEIEHAFEAALTAAA
                     TDAGKNGDRPSPTGIGMAGGFGTGKSHMLGYLAEVARQQGFVVSRVVVSKETPLSHPG
                     HVLAAALRDAALPDRPDDPIAVCVFALREQPEALEALEIAVSTPDAGFAPIFAACLFL
                     LRRASTPPEMLRRIARLWAGTKVSTPAIRKALTTAGASRMFPLKPVPAAELTNQLTRF
                     VPLLFRAAGYAGWCILLDEIELIGRYTPLQRALSYAWLAAWLGLDGEHHFPGIVIAYA
                     ITDDFATAVINARLDSEKLPERLTLKGRSAEATLAVLGIRHIERTVLQHRLLSPTLAD
                     LASCHDKVKRLYSSAYNWPAPALPAAERTSSRTMRQYIKGWITQWDLLRLDGRDVVLV
                     SGGIASNYTEDPTLTEPRAPDDEEA"
     gene            complement(3234..4190)
                     /locus_tag="VGC82_16995"
     CDS             complement(3234..4190)
                     /locus_tag="VGC82_16995"
                     /inference="COORDINATES: protein motif:HMM:NF022376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HEY0425019.1"
                     /translation="MDVLMTPPLPLAEWLLVVEREYLSEFVPAGGAAVKFAILASDAI
                     DTAAACLVRLGQTQSMLTVHVDAGRTRIHLAHELFFTIARALPWDSLLQRYLEELFSV
                     HAYPWPRPGVMMALADLAAAFAVAPNLLSRHLDQWLTADLWDDRRLAQDFRAAMLWLC
                     LSRLEPDGEDRAEPVMQWLRGEKVAPASLRMADISVRISRTNARPMLVSLCHFLRKAG
                     AAGLVVVLDVRQLARTAHVEDTLRYSPAAVMDSYEVLREIIDDAEHLPGLFIVVLADA
                     ALATGDPRRALGQYAALQMRVWPDVRPGDRQNPVAPLVWLTT"
BASE COUNT          822 a         1372 c         1380 g          716 t
ORIGIN      
        1 acaggtccaa ccgccgcaag gcgctgcgga cggtctcacg gcagcagcgg cgtgcaaacc
       61 gttcgcgcac gaacgccgcc agacgccgca gtgtccagcg cggtttcgga tcggccccgg
      121 ctacgggtgg tgcggctgcg gtctgccggg cggcgcggat ttctgcgcca agcgctgctt
      181 cgatctttag gcaaaggggc ggcggccacc ggtgcgccga tatgacagcg cctcggggcc
      241 gggttcattg tagctgtgca cccagtccat gacggtttgc ggacgccggt cggtgcgttc
      301 tgcgacctga gtggcgcagg cgccctgtgc gatatcgtgc aaggccagca ttcgttcgcg
      361 ggtgcgggta tgggcggcgt tcaaggcgat gtgcctcagg tcggccgggg tttggcccca
      421 gcgatcataa tcaacgcgta acatcagttc tgacgtgatc ctatcaagga aaactcgcaa
      481 tgtgggaaca gttcgccaca cagccaaggg cccgactagc ggtctctcaa actggacgcg
      541 gtttaatatc taaaactcgc aaccatctaa ttcggttatc gaaaccagac cacctgtcac
      601 gcaccaacgg catgcaacaa cgtcggcaga tccaaacggc gcaggccatc agttgccaac
      661 ccgtctataa tatcggcgaa cagtgcacgc ttctcgtcaa ggatctcgcc gatccgttgc
      721 tcgatactgt ctggggtgag gtaggcgaac acctgcaccg cgcgggtttg cccgatccga
      781 ctgacgcgat cctcagcctg cgtctctact gccggattcc accaacggtc aaagtgaaat
      841 accactgagg cagccgtcag attcaacccc acacccccga cccgaagtga ggaaaccaga
      901 acatgccggt ccgggctggc tgcgaacagt gctattgcgt cgtcgcgcgc ggcagcggac
      961 atgcggcctg tcagcagcag cgggttgagc ggcttgagcg cctgggcgat tgcctctgca
     1021 ccaaacggca tggcaacgaa ctgggtgaac accaacgcct tctcgccagc ttgggccaca
     1081 atgtccaatc ggcgggcgag atcggcaagc ttagcggacg cgccagtacg cgggcacgca
     1141 ttgcagatct gcttaaggcg cagaatcagc tccaggacat gggtcacgcg taattccgcc
     1201 cccagcgagc gaagccagac cagtccctcc cggcgggcga tgtcatatgc ggcgcgctgg
     1261 acgggcagta gatccggggc gatctcgaat accgtcttcg gtggtagttc gggcagcacg
     1321 tcggtgcgcc gacgacgaag ctgcaccaag gacaaagctt tgcgcagtcc tacgaccatc
     1381 tcagccgggt cacagagacc aggtgcaacg aattcaagaa tcgaaaccgt atcgtccgga
     1441 cagttctcca gcggcgtgcc ggtcagcgcc caggcgcggc gccgaggcaa ggctttgacc
     1501 gtggcggcaa tggcggtatg tgcgttcttg atgcgttgcg cttcgtcggc gaccacaacc
     1561 tgccacagtc gccgccgcgg gccatacggg gcgggcaggg ccatatcgcc gcgcagcgca
     1621 tcgaagcccg tcaggtatac ttgtgccggc gccagccagc gttgacgccg ttcctccggc
     1681 ccaccaatac atgtggacaa cgttagctca ggtgcccatt cacggaattg acgccgccat
     1741 tgcagtacca gtccggttgg caccacaacc agcgccggtc cttgtgcgcc gcgttgcagc
     1801 agcacgcgca gagcgccgat cgcctggact gtcttgccca accccatctc gtcggccagc
     1861 agcacccccg gttccgccaa aagacgcgcg atgccggcct tctggtaggg cggcaaaggt
     1921 gagcgccaat ggaatccctc ggttgaaagc gtcgcggcga gcgggacaag gtccatcaat
     1981 ccgctatgcc tcctcatcgt caggggcgcg cggctccgtc agagttggat cctcggtgta
     2041 gttgcttgcg ataccgccgg agacgagcac gacatcgcga ccgtcgaggc ggagcaaatc
     2101 ccactgcgtg atccagccct tgatgtactg tcgcatcgtc cgcgagctgg tccgctccgc
     2161 tgctggcaac gctggcgcgg gccagttgta ggcagaactg tacaggcgtt tcaccttgtc
     2221 gtggcaggag gccaggtcgg ccagcgtcgg cgatagcaag cggtgctgca gcaccgtgcg
     2281 ctcgatgtga cggatgccta acaccgccag cgtcgcctcg gcggagcgtc ctttcagcgt
     2341 cagcctttcc ggcagcttct cactgtccag gcgggcgttg ataaccgccg ttgcgaagtc
     2401 gtcagtgatt gcgtaggcga tgacaatgcc tgggaagtgg tgctcgccgt ccaggccgag
     2461 ccaggcggca agccaagcgt aggacagagc gcgttgcagc ggggtgtagc ggccgatcaa
     2521 ttcgatttcg tcgagcagta tgcaccaacc agcatagccg gctgcacgaa acaacagtgg
     2581 cacgaaacgg gtgagctggt ttgtcagctc ggcggcgggg accggcttga gcggaaacat
     2641 cctgctggcc ccagcggtcg taagggcctt gcggattgcc ggggtgctga ccttggtgcc
     2701 ggcccacagg cgcgcgatgc ggcgcagcat ttcgggtggt gtggaagctc ggcgaagcag
     2761 gaacaggcag gcagcgaaga tcggcgcgaa gccggcatcg ggcgtgctga cggcgatttc
     2821 cagcgcctcc agggcctctg gctgttcgcg cagggcgaag acacagaccg caatagggtc
     2881 atccggccgg tccggcaatg ctgcgtcgcg cagggcagcg gcgagtacat ggcctggatg
     2941 cgacagcgga gtttccttgc tgacgacaac gcggctgacc acgaagccct gctgccgcgc
     3001 gacctcggcc agatagccga gcatgtgtga tttgccggtg ccgaaccccc cagccattcc
     3061 gatacccgtg gggctaggcc gatcgccatt cttcccggca tcggtcgcag cggcagtcaa
     3121 ggccgcctcg aaagcgtgtt cgatttcggt ctgctcggtg cccatctgcc ggattgcggc
     3181 ccgattgggc acgccagccc gcagcgcctc gatcgccatg cggaactcga ccgtcacgtt
     3241 gtgagccaca ccaacggggc cactgggttc tgccggtcgc ccggccgcac gtcgggccaa
     3301 acacgcatct gcagcgcagc atattggccg agcgcgcgac gcggatcgcc ggtagccagc
     3361 gccgcgtcgg ccaaaaccac gatgaacagg ccaggcaggt gctcggcatc gtcgatgatc
     3421 tcccgcaaga cctcgtagct gtccatcacc gcagccgggg aatagcgcag agtatcctcg
     3481 acatgtgcag tgcgtgccag ctgtcgcacg tccagcacaa cgaccaaccc ggcggcgccg
     3541 gccttgcgca ggaaatggca cagggaaacc agcatggggc gtgcattggt gcgggagatg
     3601 cgaaccgaaa tgtccgccat gcgcagtgag gcgggtgcca ctttctcgcc gcgcagccac
     3661 tgcatgaccg gttcggcgcg gtcctcgccg tccggttcca gccgtgacag gcagagccac
     3721 aacatggccg cgcggaaatc ctgcgccagc cggcggtcat cccaaagatc ggcggtcagc
     3781 cactgatcca ggtggcggct cagcaggttc ggcgccacgg cgaacgcggc ggccagatcg
     3841 gccagcgcca tcattacacc cggccgcggc cacggatagg catgcacact gaaaagttct
     3901 tccagataac gctgcaggag gctgtcccag ggcagggcgc gggcgatggt gaaaaacagc
     3961 tcatgcgcta agtgtatccg cgtgcgtccc gcgtcgacat gcaccgtcag catggactgt
     4021 gtctgtccaa gccggacgag acaagccgcg gccgtgtcga tggcgtcgct ggcgaggatg
     4081 gcgaatttca cggcagcacc gcccgccggc acgaactccg acaaatattc gcgctccacg
     4141 accaacagcc actcagccag cggcagcggc ggtgtcatca gcacgtccat ggggctattc
     4201 caggatgaag cggatagcga agtagtcgtg ctgctcgccg gtctcgtcaa acaatgtcaa
     4261 ccgtttgccc cccttgcggc cagtggagcc
//
LOCUS       DASWQN010000994         1926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2075052, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000994 DASWQN010000000
VERSION     DASWQN010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075052"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VGC82_17000"
     CDS             <1..551
                     /locus_tag="VGC82_17000"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0425020.1"
                     /translation="NPKFDPVWKKAEELGAVLFIHPQGVPELSKRWKGNGWLANTIGN
                     PLATTIALQHLIFDGSFDKFPGLKVLAAHGGGYLASYAPRGDHACTVSPTQCDPNIKL
                     KKKPTEYLNQIYFDSLIFTPEALRHLVAQVGSSQVVLGSDYPYIWELHPVDHVLATKT
                     LSDKEKVAILGGNAARIFGMKS"
     gene            complement(924..>1926)
                     /locus_tag="VGC82_17005"
     CDS             complement(924..>1926)
                     /locus_tag="VGC82_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HEY0425021.1"
                     /translation="DTQTLPSAAMKEAMMRAEVGDEQSGDDPTVNALCDRMAALTGKQ
                     AAMFLPTGTMCNQISILVHCRPGDEILAHEDSHIIINEGGGPGALAGAVVLGLRGARG
                     MFDAATLTAALRERRRNAPPQTLVAVEQTANAGGGSVWPVALLNDVLGAAHDQDMKTH
                     MDGARLMNAVAAARVTVAEMTQHCDSVWLDFTKGLGAPLGAVLCGSEEFIDEAWRWKQ
                     RLGGSMRQAGICAAACLYALDHNVDRLAEDHDNAQALARGLAQVPGLTVEEPETNLVF
                     FDTRGTGLTADALAERVRQLGIKVSTVGPYRMRACTHLDVDAAAIELAMQVFAEVVR"
BASE COUNT          369 a          652 c          577 g          328 t
ORIGIN      
        1 ccaacccaaa gttcgatccg gtgtggaaga aggcggagga gctgggcgcc gtcctgttca
       61 tccaccccca gggcgtgccg gagttgagca agcgttggaa aggaaacggc tggctggcca
      121 atacgatcgg caatccactg gccaccacaa ttgcgctgca gcacctgatt ttcgatggtt
      181 cgttcgacaa gttccccggc cttaaggtgc tcgccgcaca tggcgggggc tatttggcat
      241 catacgcacc gcggggcgac catgcctgca cggtgtcacc cacgcagtgc gacccgaaca
      301 tcaagctaaa aaagaagccg acggaatacc tgaaccagat ttacttcgac tccctgatct
      361 tcacgcccga agcgctgcgt cacctggtcg cgcaagtcgg ctccagccag gtcgtgctcg
      421 ggagcgacta tccctatatc tgggagctac acccggtgga ccatgtcctt gcaacgaaaa
      481 ccctgagcga taaggagaag gtcgcgatcc ttggaggcaa cgccgcccgc atcttcggca
      541 tgaagtcata acgcgcccct ccgaacgggg ctcacaggcg cggaaacggg gctcctagcg
      601 tgcccaaggc cctggttgat accaggggca aggccgtggt cgatgccatg ggcgaggcag
      661 tggttgataa tagtgtcatg gccgtgcttg gcgcggccac ccacgacttt gtctgcacgt
      721 ggaaggttgt ggatcgccag cccgggctgg tcaagacgca ggcaacaaaa ccacaaacgg
      781 cgctaatctc acaccgaggc gaaccccggc ggcgcgaacc gcggcccgac atgctgggca
      841 agatcactgc tcgaagccgt tatgcggtca tcggtccctt acaccggccg ccttggcggc
      901 ggcgaaatcc tgaggctgcg ccgctaccgc actacctcgg caaacacctg catcgcgagt
      961 tcgattgctg ccgcgtccac atccagatgc gtgcaggccc gcattcgata cgggccaacc
     1021 gtcgagacct tgattccaag ctggcgcacc cgttcggcca gggcatccgc cgtcagtccg
     1081 gtgccgcgcg tatcgaagaa caccaggttc gtttccggct cctccaccgt cagccccggc
     1141 acctgcgcca ggccgcgcgc aagggcttgg gcattgtcgt ggtcctccgc cagccgatcc
     1201 acattatggt ccagtgcata gaggcaggcc gcggcgcaaa ttcccgcctg gcgcatcgaa
     1261 ccgccaaggc gctgcttcca gcgccaagcc tcgtcgatga attcctcgga cccgcaaagc
     1321 acggcaccga gcggcgcccc aaggcccttg gtaaagtcca gccacaccga atcgcaatgc
     1381 tgggtcattt ccgcgacagt cacccgggcg gcggcaaccg cgttcatcag ccgcgccccg
     1441 tccatatgag tcttcatgtc ctgatcatgg gcggcaccta gcacgtcgtt cagcagcgcc
     1501 accggccaaa ccgacccgcc gccggcgttg gcggtttgct ccaccgccac caaggtctgc
     1561 ggcggggcgt tgcggcgccg ttcccgcagt gctgccgtca gcgtggcggc atcgaacatc
     1621 ccgcgtgcgc cgcgaaggcc gagaacgacc gcaccagcca aggcaccggg gccgccacct
     1681 tcgttgatga tgatatgcga atcctcatgc gccaggatct cgtcgcccgg gcggcaatga
     1741 acaaggatgg agatctggtt gcacatcgtc ccggtcggca ggaacatcgc cgcctgcttg
     1801 ccggtcaacg ccgccatccg gtcgcataac gcgttcaccg tgggatcatc gccggattgt
     1861 tcgtccccga cctcggctcg catcatcgct tccttcatcg cggcggacgg aagcgtctgg
     1921 gtgtcg
//
LOCUS       DASWQN010000995         2880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1942946, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000995 DASWQN010000000
VERSION     DASWQN010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942946"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2013
                     /locus_tag="VGC82_17010"
     CDS             <1..2013
                     /locus_tag="VGC82_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019084967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEY0425022.1"
                     /translation="FPGGIPSHAAPDTPGSIHEGGELGYSLSHAFGAVLDNPDLIAAC
                     VVGDGEAETGPLATSWHGNKFNSARTDGAVLPILHLNGYKIANPTVLARIPSAELHAL
                     MTGYGYDPIEVAGSDPAEMHQAMAAAMDWAFDRIRTIQLAARGGGETSRPQWPMIILR
                     SPKGWTGPKVVDGLKSEGFWRAHQVPFTMDKPEHLKLLDDWMRSYRPEELFDNAGRPI
                     EAITGLAPSGDKRMSANPHTNGGVLMRPLRMPDFTKHALKVPSPGAVDGESTRVMGAF
                     LRDVMAANADTRNFRVFSPDEHNSNRLNDVLEVTNRAWDAETIPDDDHLAPDGRVMEI
                     LSEHTCQGWLEGYLLTGRHGLFSCYEAFIHIVDSMLNQHAKWLKSSRAISWRRPIASL
                     NYLLTSHVWRQDHNGFSHQDPGFIDHVINKKADVARVYLPPDANTLLWVTDHCLRSWD
                     RINVIVAGKQPEPQWLDMESAIKHCTQGIGIWPFASNDQDSTPDVVMGCAGDVPTLEV
                     MAAVDLLHGLLPELKIRVVNVVDLMTMQPKSQHPHGLSDEDFDALFTTDGPVIFAYHG
                     YPSLIHRLVYKRKNHGNFHVRGFMEEGTTTTPFDMVVRNRLDRFHLVADVIDRVPGLS
                     ARAVYAKQAIRDKLIDHEQYIVRHGKDMPEVSEWRWPSQARHGTSA"
     gene            2141..>2880
                     /locus_tag="VGC82_17015"
     CDS             2141..>2880
                     /locus_tag="VGC82_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425023.1"
                     /translation="MKVQAMTDLVIKFLPAKPKRWRGTAVVLGAALSVVVPTAVQAQS
                     IGTGPASAINHANSRQNPNREKPELPAPDAIPGAKAREPAAPATRMATDMPPTDALFD
                     AINRGDIASARDALNRGADLNGMNILGMTPMELSVDLGRNDISFLLLSMRGEDSGRGS
                     RAVGRDVTAAGKMAQATKPATSARTSGAGAKLAGVKVGGAKPVGAQSVGAQSVAGRPV
                     ATPKLFADDGGTPLPSAGFLGFGSRSAT"
BASE COUNT          550 a          897 c          931 g          502 t
ORIGIN      
        1 tttccgggcg gcattcccag ccacgcggcg cccgatacgc caggatcgat ccacgagggc
       61 ggcgagcttg gctactcgct atcccatgcg ttcggcgcgg tgctggacaa tcccgacctg
      121 atcgctgcct gcgtcgttgg cgacggagag gcggaaaccg ggccgctggc cacgtcctgg
      181 cacggcaaca agtttaacag tgcgcggacc gacggcgccg tgctgccgat cctgcatctg
      241 aacggttaca agatcgccaa cccgacggtc ctggctcgca tcccgtccgc cgagttgcat
      301 gccttgatga ccggctacgg ctatgacccg atagaggtcg ccgggtcgga cccggccgag
      361 atgcatcagg cgatggccgc cgcgatggac tgggcgttcg acaggatccg gaccattcag
      421 cttgcggcgc gtggcggcgg cgagaccagc cgtccgcaat ggccgatgat catcctgcgc
      481 agcccgaagg gctggaccgg ccccaaggtc gtggacggct tgaagagcga ggggttctgg
      541 cgggctcatc aagtgccctt caccatggac aagccggaac acctgaagct gttggacgac
      601 tggatgcgga gctaccgccc cgaggaactg ttcgacaacg ccgggcggcc gatcgaggcg
      661 atcaccggtt tggccccgtc cggggacaag cgcatgagcg ccaacccgca caccaacggc
      721 ggagtgctga tgcgcccgct gcggatgccg gacttcacca aacacgcgct caaggttccc
      781 agtcccggag cggttgacgg ggaatccacc cgcgtcatgg gcgcattcct gcgcgacgtg
      841 atggcggcca acgccgatac ccgcaatttc cgtgtcttct cgccggacga acacaactcc
      901 aatcggttga acgacgtgct ggaggtcacc aatcgggcgt gggatgcgga gacgataccg
      961 gatgacgacc atttggcacc cgacgggcgg gtcatggaaa tcctcagcga gcatacctgc
     1021 cagggatggc tggaagggta tttgctgacc ggccggcacg ggctgttttc atgctatgag
     1081 gcgttcatcc acatcgtcga ttcgatgttg aaccaacacg ccaagtggct gaaaagttcg
     1141 cgcgcgattt cctggcgccg gccaatcgcg tcgctgaact accttctgac gtcgcatgtc
     1201 tggcggcagg accataacgg gttcagccat caggaccccg gcttcatcga tcacgtaatc
     1261 aacaaaaagg ccgacgtcgc gcgggtgtac ctgccgcccg acgccaacac cttgttgtgg
     1321 gtgacggatc actgcctgcg aagctgggac cgcatcaacg tgatcgttgc cggcaaacag
     1381 ccggagccgc aatggctgga catggagtcc gcgatcaagc actgcaccca gggcatcggc
     1441 atctggccct ttgccagcaa cgaccaggat tccactccgg atgtcgtcat gggttgcgcc
     1501 ggcgatgtgc cgacgctcga ggtgatggcg gcggtggacc ttctgcacgg attgctgccc
     1561 gagctgaaaa ttcgcgtcgt caatgtcgtc gatctgatga ccatgcagcc aaaatcgcag
     1621 cacccgcacg ggctgagcga tgaagacttc gacgcgctgt tcaccaccga cggtccggtg
     1681 atcttcgcct accacggcta tcccagcctg atccaccgct tggtttacaa gcggaaaaac
     1741 cacggcaact tccatgtccg cggcttcatg gaagagggca cgactaccac gccgttcgac
     1801 atggtggtac gcaaccggct cgatcgcttt catctggtgg ccgacgtcat cgatcgcgtt
     1861 cccgggctga gcgcccgggc ggtttatgcc aagcaggcga tccgcgacaa actgatcgac
     1921 cacgagcaat acatcgtgcg gcacggcaag gacatgcccg aggtttcgga atggcgttgg
     1981 ccttcgcagg cgcggcacgg gacttccgcg taaaaccgcg ccggaggacc ttttgggcac
     2041 aggctatttt cgggctgcac agcgatccga caaatgagct acggaactgc cttcgatgtt
     2101 caacgatggc tgaggccata aagaccaagg ggctgatgcc gtgaaagttc aggcaatgac
     2161 cgatctggtg ataaaattct tgcccgccaa gccgaagcgc tggcgcggga cagccgtggt
     2221 gctcggggcg gcgctgagcg tggtcgtgcc gaccgcggtt caggctcaat cgatcggcac
     2281 gggacccgcc tcggccatca accatgccaa ctcacgccaa aaccccaacc gtgagaagcc
     2341 ggagctgccg gctccggatg cgattccggg tgccaaggcg cgggaacccg ccgcgccggc
     2401 gacccgcatg gccaccgaca tgccgccgac cgatgctttg ttcgatgcga tcaaccgggg
     2461 tgatatcgcc tcggcgcgcg atgcgcttaa ccggggcgct gatctgaatg gcatgaatat
     2521 ccttggaatg acgccgatgg agctttcagt cgatttgggt cgcaacgata tttcgttcct
     2581 gctgctttca atgcgaggcg aggatagcgg ccgaggctcc cgtgcggttg ggcgcgacgt
     2641 cacggcggcc gggaagatgg cccaggccac caagcccgcg acttcggcca gaacttcggg
     2701 agctggggcc aagcttgcgg gggtcaaggt tgggggtgcc aagcctgtcg gagcgcagtc
     2761 tgttggagcg cagtctgttg cgggcaggcc tgtcgcgacg ccgaagcttt tcgccgacga
     2821 cggggggacg ccgctgccca gcgcgggatt cctgggcttt ggctcgcggt ccgcgacgaa
//
LOCUS       DASWQN010000996         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1678898, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000996 DASWQN010000000
VERSION     DASWQN010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678898"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VGC82_17020"
     CDS             <1..1147
                     /locus_tag="VGC82_17020"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEY0425024.1"
                     /translation="DIRGFALKFYTEEGNWDIVGNNTPVFFFRDPLRFPDLNHAIKRD
                     PRTGMRSADNNWDFWTLLPEALHQVTIVMSERGIPRSFRHMHGFGSHTYSFINGTNER
                     TWVKFHFRTQQGIKNLTDAEAEALVGKDRESHQRDLFESIEARDFPRWTLFIQVMTDD
                     QARAFPFNPFDLTKVWPKGDFPLIEVGFYELHRNPENVFAEVEQVAFTPANIVPGIGY
                     SPDKMLQARLFSYGDAQRYRLGVNHHHIPVNAPKCPVHSYHRDGAMRTDGNLGATPTY
                     FPNSRGEWIDQPQFDEPPLEISGAAAHWDHRVDDDHWQQPGDLFRKMTQQQKQLLFDN
                     TARQVGGAAKHIQERHVANCAKADPAYGAGVAKALGIGFKPQTVAAE"
     gene            1271..>1634
                     /locus_tag="VGC82_17025"
     CDS             1271..>1634
                     /locus_tag="VGC82_17025"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEY0425025.1"
                     /translation="MSDRIAPKLLTEFIGTFALVFIGAGAAAVVGDGVGLSGITAIAF
                     AHGLTIMVFAFAYGSISGGHMNPAVTVGVLAVGAMGVGEAIGYIISQLIGGIAGAHFL
                     SIVLGGAETGLGTPALAHN"
BASE COUNT          317 a          537 c          475 g          305 t
ORIGIN      
        1 cgacatccgc ggcttcgcgc tgaagttcta taccgaggaa ggaaactggg acatcgtcgg
       61 caacaacacg ccggtgttct ttttccgcga tccgcttcgc tttcccgatc tcaaccacgc
      121 catcaaacgc gatccgcgaa ccggtatgcg cagtgccgac aacaactggg acttctggac
      181 cctgctgccg gaagctctgc accaggtcac gatagtcatg agcgagcgcg ggattccccg
      241 gagtttccgc catatgcatg gcttcggcag ccatacctac agttttatca acggcacaaa
      301 cgagcgcacc tgggtcaaat tccatttccg cacccagcag ggcatcaaga acctgacgga
      361 tgccgaagcc gaagccttgg tgggcaagga ccgcgagagc catcaacggg accttttcga
      421 aagcatcgag gcccgggatt ttccgcgctg gaccttgttc atccaggtga tgacggatga
      481 ccaggcgagg gcatttcctt tcaatccctt cgacctgacg aaggtctggc caaagggcga
      541 tttcccgctg atcgaggtcg gtttctatga gctccatcga aatccggaaa acgtcttcgc
      601 cgaggtcgag caagtggcgt tcacacctgc caacatcgtg cctggcatcg gctattcgcc
      661 ggacaagatg ctgcaggcgc gcctgttctc ctatggcgat gcgcagcgct atcgcttggg
      721 cgtcaaccac caccacatcc ccgtcaacgc accgaaatgc ccggttcaca gctatcaccg
      781 ggacggcgca atgcgcaccg acggcaatct cggggccacg ccgacctact ttccgaacag
      841 ccgcggcgag tggattgacc agccgcagtt cgacgagccg cccctcgaga tcagcggcgc
      901 tgccgcccac tgggatcacc gggtggatga cgatcactgg cagcagccgg gcgatctgtt
      961 ccggaagatg actcagcaac agaagcagtt gctgttcgac aataccgccc gtcaggtcgg
     1021 cggcgctgcg aagcacatcc aggagcgcca tgtcgccaat tgcgccaagg ccgatcccgc
     1081 ctacggcgcc ggagttgcga aggcgctggg catcggtttc aaaccgcaga cggttgccgc
     1141 ggagtaggtc ccgctcgcct tacgggccgg cggcgctctc cctgccgccg gcaatccccc
     1201 aaatcaaccc aatcttcgtt ggcgcaaaac ctggcggcgc ccagggacca accttatacc
     1261 ggagagaacg atgtccgacc ggattgcacc caagctcctc acggagttca tcggcacctt
     1321 cgcactcgtg ttcatcggcg ctggcgcggc ggccgtggtc ggcgacggtg tcggcctgtc
     1381 aggtatcacc gcgatcgcct tcgcccacgg cttgaccatt atggtcttcg ctttcgctta
     1441 cgggtccatt tcgggcggcc atatgaatcc agccgttacc gtcggcgtgc tcgccgtggg
     1501 cgcgatgggc gtcggcgagg cgattggcta catcatcagc caactcattg gtggcatcgc
     1561 gggcgcgcac ttcctgtcga tcgttctggg aggcgccgag acgggtctcg gcacgcctgc
     1621 acttgcccac aacc
//
LOCUS       DASWQN010000997         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1678990, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000997 DASWQN010000000
VERSION     DASWQN010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678990"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VGC82_17030"
     CDS             complement(<1..366)
                     /locus_tag="VGC82_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425026.1"
                     /translation="MRRAIVILVVFLNAPAWGHEDLVLPAVEFAATAVHESGAFRSTE
                     TVHYASGKLRIDRGSGFSSTILDLTTQTQCLLMANHTYLIMPMDDELFRRYIARIVDV
                     NGARAKGTERIEGLETTRYV"
     gene            complement(529..1341)
                     /locus_tag="VGC82_17035"
     CDS             complement(529..1341)
                     /locus_tag="VGC82_17035"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0425027.1"
                     /translation="MPIIDAQVHIWGSGKPSGHHRQTSVFTARELILEMGQAGVDGAV
                     LHPPSWDPKSNEMAIEAAVDSPDRFCSLGWFPLDDPSQRKRIETWKQQPGMLGLRWSL
                     TREGQETWHEDGTMDWLWPAAEKAGTPVATMAWRFLPMFGQIAERHPHLKLIVDHLGL
                     VRSAKGQAAFATLDELLKLAKLPNVAIKATGAPGYSNEAYPFRDIQDGLHRIFDSYGP
                     DRFFWGTDITRMPCSYRQCVTFFTEELPWLKGKDLEMVMGQAVIDWLGWKRG"
     gene            1445..>1561
                     /locus_tag="VGC82_17040"
     CDS             1445..>1561
                     /locus_tag="VGC82_17040"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY0425028.1"
                     /translation="MQTVLDRVLLTNDDGIDAPGMQVLEEIAALIAREVWVVA"
BASE COUNT          296 a          495 c          454 g          316 t
ORIGIN      
        1 gacgtatctg gttgtttcca aaccctcaat ccgctccgtc cccttcgctc gggcgccatt
       61 tacgtcaacg attcgcgcga tgtagcgtcg gaacagctca tcgtccatcg gcatgataag
      121 ataagtgtga ttggccatga gcaggcactg agtctgggtc gtcaagtcca ggatggtgga
      181 gctgaatccg ctaccgcgat cgatgcgcag tttgccgctg gcgtaatgga ccgtttccgt
      241 gcttcggaac gcccctgact cgtgcaccgc ggttgcggcg aattcgacag ccggcagcac
      301 caggtcctcg tgcccccaag cgggcgcatt gaggaaaaca accaggatca ctatcgcgcg
      361 ccgcatcgac atcacgctaa ccaccaggac gacatcgtga ggcctagttc gttgccttgt
      421 caatccacaa aacagtgcgg ccggccaatg atcggccacc acaggtagtc gcgtggttcg
      481 gcagggtcag gcgctattgc cgcgtcttca tcacctgcca gggatcgtct agcctcgctt
      541 ccatcccagc caatcgatca ccgcctggcc catgaccatc tccagatctt ttccctttag
      601 ccaagggagt tcctcggtga agaacgtcac gcattgccgg taactgcacg gcatccgcgt
      661 gatgtctgtt ccccagaaga accgatccgg accgtacgag tcaaaaatcc ggtgcagccc
      721 gtcctggatg tcgcggaatg gataagcctc gttcgaatag ccgggggcgc cggtcgcttt
      781 gattgccacg ttcggcaact tcgccagctt cagcagctcg tccagggtcg cgaacgccgc
      841 ctggcccttt gccgatcgca ccagtccgag atgatcgacg atcagcttca gatgcggatg
      901 ccgttcggcg atctgcccga acatcggcag gaagcgccag gccatcgtcg caaccggcgt
      961 accggctttc tccgcggcgg gccacagcca gtccatggtg ccgtcttcat gccaggtttc
     1021 ctggccttcc cgggtcagtg accagcgtaa gccaagcatc ccgggctgct gcttccaggt
     1081 ttcgatccgt ttgcgctgcg acgggtcgtc cagcgggaac catcccagcg aacagaatct
     1141 gtccggagaa tcaaccgcgg cctcgatcgc catttcgttg gatttcggat cccagctagg
     1201 cggatgcaaa acggcgccat cgaccccggc ctgacccatc tccaggatca attcccgggc
     1261 cgtgaacacc gaggtctggc gatgatgccc gctaggcttg ccgctgcccc agatgtggac
     1321 ctgagcgtca atgatcggca ttggatgttg tccccctttt tccggatggt gggctttttc
     1381 cggatggtgg cctcttgccg gccggtgtgc aaccgggcca aacagggcaa cgacgaagac
     1441 gggaatgcaa acagtgctcg atcgggtctt gctgacgaac gacgacggga tcgatgcccc
     1501 gggtatgcaa gtgctggagg aaatcgccgc cctcatcgcc cgagaggtct gggtcgtcgc
     1561 g
//
LOCUS       DASWQN010000998         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1415858, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000998 DASWQN010000000
VERSION     DASWQN010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415858"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VGC82_17045"
     CDS             complement(<1..263)
                     /locus_tag="VGC82_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425029.1"
                     /translation="MRPEQVIAAIRFDPLLPVWMLVVLSVLALVVVGVGFWRRGKGAA
                     LRLGAFAVLLLWLAGPRLVQETRQSLRDIGLLVVDQSASMQVG"
     gene            complement(264..>1565)
                     /locus_tag="VGC82_17050"
     CDS             complement(264..>1565)
                     /locus_tag="VGC82_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEY0425030.1"
                     /translation="GRSWATLEDGTPLVTEAGFGAGRVVLFHVTANADWSNLPLSGLF
                     VEMMRRLVDLSVGIAATQGSAVLSPAETLDGFGLLSQPPPAAIGLQADKFGSTPVSPR
                     HPPGLYGPESGRQVLNLGTSIAAPEPAPPIAGATLETVRGSARERALGPPLLAFAILL
                     LCVDMIVSLALRGLLRARVSATAAALLLLIVPAAHALDSSPSPATDTRLGYISSGDGQ
                     VDNVAKAGLEGLSDYVNHRTAASLFEPDAVQPGQTDLSPYPLLYWPITAEAQELTSTQ
                     SAALNDYMSRGGIILIDTRDSGSGAGFAPGTENALRRIARSLMVPPLAPLSSEHVLSH
                     SFYLLSDFPGRFTGDTVWVQRDQDRTNDSVSPVIIGGNDWAGAWAVGSDGSNPYAVIP
                     GGQRQRTLAYRFGVNLVMYALTGNYKGDQVHVPAILQRLGQ"
BASE COUNT          314 a          548 c          484 g          219 t
ORIGIN      
        1 tcgccgacct gcatggaggc cgactggtcc accaccagca gtccgatatc acgcagcgac
       61 tgccgcgttt cctgcaccag cctgggcccc gccaaccaca gcagcagcac cgcgaatgca
      121 cccaaccgca atgccgcgcc cttgccccgc cgccagaaac ccacgcccac gacaaccagc
      181 gccagcacgg acagcacgac cagcatccac accggcaaaa gcggatcgaa gcggatcgcc
      241 gcgatgacct gttcaggacg catctactgg cccaaccgct gcaggatggc cggcacgtgc
      301 acctgatcgc ccttatagtt gccggtcagc gcatacatca ccagattgac cccaaaacga
      361 taggccaggg tccgctgccg ctgcccgccg ggaatcaccg catacgggtt gctgccgtcg
      421 gatccaacgg cccaggcacc ggcccaatcg ttgccgccga tgattaccgg actgacgctg
      481 tcgttggtgc gatcctggtc gcgctgcacc cacaccgtat cgcccgtgaa acggccggga
      541 aaatccgaca gcaggtagaa cgagtgcgac agcacatgct cgctggacag cggcgccagt
      601 ggcggcacca tcagcgagcg ggcgatccgg cgcaaggcgt tctccgtgcc gggagcaaag
      661 ccggcccccg atccggagtc gcgggtatcg atcaggatga tcccgccacg gctcatgtaa
      721 tcgttcaacg cggcggactg ggtgctggtc agttcctggg cctcggcggt aatcggccaa
      781 tacagcagcg gatacgggct gaggtcggtc tgtccgggct gcacggcatc cggctcgaac
      841 agcgaagccg ccgtgcggtg gttgacgtaa tccgaaagtc cttccagtcc ggccttggcg
      901 acgttgtcca cctggccgtc gccggacgag atatacccca atcttgtgtc ggtcgccgga
      961 cttggactgc tgtccagggc atgcgcagcc ggaacaatca acagcagcag tgcggcggcg
     1021 gtcgccgaaa cccgagcccg caacaagccg cgcaacgcca gcgacacgat catgtccacg
     1081 cacagcagca gaatcgcaaa ggccagcagg ggtggcccga gcgcccgctc gcgcgccgac
     1141 ccgcgaacgg tttcaagcgt cgcgccggcg attggcggcg ccggttccgg cgctgcgatg
     1201 ctggttccaa ggttcagcac ttgccgcccg ctttccggtc catacaatcc cggtggatgc
     1261 cggggcgata ccggggtcga gccgaacttg tcggcctgca ggccgatggc ggccggaggc
     1321 ggctgcgaca gcaaaccgaa cccgtccaaa gtctcggcgg gggaaaggac ggcgctaccc
     1381 tgagtggcgg caatacccac ggacaggtca accaggcggc gcatcatctc cacgaacaaa
     1441 ccggacagcg gcaggttaga ccagtcggca ttggcggtca catggaacag cacgacgcgc
     1501 ccggcgccga atccagcttc ggtgaccaaa ggagtaccgt cctccagcgt cgcccaactc
     1561 cgccc
//
LOCUS       DASWQN010000999         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1943481, whole
            genome shotgun sequence.
ACCESSION   DASWQN010000999 DASWQN010000000
VERSION     DASWQN010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1943481"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VGC82_17055"
     CDS             complement(<1..274)
                     /locus_tag="VGC82_17055"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0425031.1"
                     /translation="MSKQHSYVLGLNTYDHDVSACLLRDGVIAFAIAKERITREKHAS
                     GFYKEVIDYCLDAEGITLDDVDLIVRNCYILPVAEMEERLVYQDMPG"
     gene            511..810
                     /locus_tag="VGC82_17060"
     CDS             511..810
                     /locus_tag="VGC82_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425032.1"
                     /translation="MAKRYNIVTRCIAAIALMFVYFVSTSAVLVGTSTTAALARGGGG
                     RGGGGGRGGGFRGGGFRGGGFRGGGRGWGRGVGVYGYGGGCHWSYRWGRWVCPYY"
     gene            complement(1020..1448)
                     /locus_tag="VGC82_17065"
     CDS             complement(1020..1448)
                     /locus_tag="VGC82_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0425033.1"
                     /translation="MPEIINFGSLQLKFLHSGEETDGDLDLFEMTLQPNARMPIPHHH
                     EGWDETVYGLAGISSWRIDGKEIDIGPGESVFIKRGIVHGFTNRSAEPATCLCILTPG
                     VLGPQYFRDMAGLLAAGTPDPQKMKETMLRYGLIPAPPPA"
BASE COUNT          300 a          475 c          506 g          336 t
ORIGIN      
        1 aacccggcat gtcctgatag accagccgct cttccatttc ggccaccgga aggatgtagc
       61 agttgcggac gatcagatcg acatcgtcca gcgtgatgcc ctcggcgtcc aggcaatagt
      121 cgatcacctc cttgtaaaaa ccgctggcgt gcttctcgcg cgtgatccgc tctttggcga
      181 tcgcgaacgc gatgacgccg tcacgcagca ggcaggcact gacatcatga tcgtacgtat
      241 tgaggccgag aacgtagctg tgttgtttgg acatggggca ctcttgcggt cgaagcatcg
      301 ccggaaatca cgcaccgcgg gcgttaatgg cgcattgcac aatgtacgaa ccccgcagtg
      361 taacttgacc tgccgaagtc cgcccaatag aatttcgaga gccgcaagaa ccgtgcctac
      421 gaccacaaat cttagcgcac ccttgatcca gctacaggtg gccccacgcg gtgtcctgtt
      481 tttcagggat ggagtgaccg ggagatgcaa atggccaaac gctacaatat cgttacgcgc
      541 tgcattgcag cgatagcctt gatgttcgtt tacttcgtga gcacgtcggc tgtcctggtc
      601 ggcacctcaa ccacagcggc cttagcccga ggcggcgggg gccgcggcgg cggcgggggt
      661 cgcggcggcg gtttccgggg tggcggattc cgtggcggcg gtttccgtgg cggtggcaga
      721 ggctgggggc gcggcgtcgg cgtttatggc tacggcggcg gttgccattg gagctaccgc
      781 tggggccggt gggtctgccc gtattattga tcgcgggcgc gatccgaaaa aataacagag
      841 acttcgcgat caagtgcgac ggccagcccg ccgcacttga tttcgttttg gagatcgccg
      901 ccgactgtcg gccttgccgc cgtcggccgt tcccaattcc gggaattggc aaggcgaact
      961 cgaccgaccc gaaagtcatc gttttgatgt aggcagccgc aaagaattgt cggcatttgt
     1021 cacgcgggag gcggcgccgg gatcagcccg taacgcagca tggtctcttt catcttctga
     1081 gggtccgggg tgccggcggc cagcagaccg gccatatccc tgaaatattg cgggccaagt
     1141 acgccggggg tgaggataca aagacacgtc gccggttcgg ccgagcggtt ggtgaatccg
     1201 tgcacaatgc cgcgtttgat gaaaacactt tcgccggggc cgatgtcgat ttccttgccg
     1261 tcgatccgcc agctcgagat gcccgcgagc ccgtacaccg tttcatccca gccctcatgg
     1321 tgatgaggga tcggcattcg cgcattcggc tgcagggtca tctcgaacag atcgagatcg
     1381 ccgtcagtct cctcgccgct gtgcaggaat tttagctgaa gtgagccgaa gttgatgatt
     1441 tcaggcatgt ctctgctctc tcattaatgc gacagggaag gaagtgctga agcccctcgg
     1501 cctgaaccgg cgcctcaccc ggcgactggc ggcgtgccgt gggtatgaaa cgactcgatg
     1561 gtcttgaggc cccaggcctg ccccttcttt cgctcctcct cggtccatac gatcggc
//
LOCUS       DASWQN010001000         6260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_360888, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001000 DASWQN010000000
VERSION     DASWQN010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360888"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1647)
                     /locus_tag="VGC82_17070"
     CDS             complement(103..1647)
                     /locus_tag="VGC82_17070"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004859.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HEY0425034.1"
                     /translation="MNAILPPIPLIPDNAPFSEEQRSWLNGFLAGLYGSAEATTPMPP
                     AAVEPEEDFPWHDPAMEMDERLALAAGKPVKRRLMAAMAQLDCGQCGYLCQTYAEALA
                     EGRETTTTLCVPGAKPTARMVKQILAAAPSAVAVPPEVAKAPAEPEGMPVHVLHSERL
                     TAPDSAKDVRHVVIDLAGSGLSYLPGDSLSLAVRNDPALIRATIAELGASGDERVTCA
                     DGTDRPLRDAFAVLDIARPLDRTMSLLAMTATYPAHAETLRKLSDGDDGIEPDCPDLL
                     DLLHAFPSARPKLADLVRSLPMLRRRLYSIASSPRAHEGQVHLCVSVVRDDRRGRVRH
                     GVGSGFLGRHAVDAGPVLASVQQSHFRLPADPRTPVIMVGPGTGIAPFRAFLADRAAT
                     GARGRTWLFFGDQHRQSDFLYGPTLEGWIKDGTLSRMDLAFSRDQKDKVYVQHRMIEQ
                     MADLWRWLQDGAHFYVCGDASRMARDVDAALRQIARAEGRLTEDQARDWIVSLARQGR
                     YLRDIY"
     gene            complement(1644..3416)
                     /locus_tag="VGC82_17075"
     CDS             complement(1644..3416)
                     /locus_tag="VGC82_17075"
                     /inference="COORDINATES: protein motif:HMM:NF007126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirA family protein"
                     /protein_id="HEY0425035.1"
                     /translation="MSAAEQPFTGRQQEYLKGFMAGVEARRAVFGMPVAPGGGAVAAD
                     PGDLQRIAQDKTIAAGGKLVPEEEAKRKKPPFRRFDEMSALASNGQFPKGTDVFLTKY
                     FGMFYVAPAQNAFMCRLRIPGGILTTAQFRGVADIADDLAGGYADITTRANLQLREIG
                     PSGPPEVLMRLAEIGLTSRGSGADNIRNITGSPTAGIDPQELIDTRPHARALHHFILH
                     HQELYGLPRKFNIAFDGGGRIAVLEDTNDIAFTAVTVEDGAAVPPGVYYRLGLGGITG
                     HRDFARETGVVVPPDDTSRVSNAVLRVFIAEGNRTDRNKARLKYVLDRIGMPSFLELV
                     EAELGEPLRRVDAAAIRQAPPQDRLGHIGVHPQKQFGRCYIGITCPVGRLTSEQMRGL
                     AAIADCLGCGEIRLTVWQNLLIPHVAEADVAAAVAAIEALGLSTEASRLRTGLVACTG
                     NAGCKFAASDTKGHATALVDYLEERIAVDAPINIHLTGCHNSCAQHYIADFGLLGAKV
                     EQGEDMVEGYDLHVGGGAGADQAIGRLIRPGIPFPELPPMILALLSSWMNERGAGETF
                     RAWTAARDDAALAAIIDLVPAEAA"
     gene            complement(3421..4254)
                     /locus_tag="VGC82_17080"
     CDS             complement(3421..4254)
                     /locus_tag="VGC82_17080"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEY0425036.1"
                     /translation="MDSVSPAAIVAAMVQSGTAKAQLGPADLLVRGTLSGALLGIATS
                     LALGAAVQTGQLLVGALIFPVGFVMIVLLGLELVTGSFAVVPMAVMAGQVKPGQMLVN
                     WSWVLSGNLIGSVIYGLLFVIAVTDAWQIAPAGVAERVRQLAEAKTIGYAAFGSAGML
                     TVFVKAMLCNWMVCMGVVMGLCSTSVIGRIAAAWMPILVFFAQGFEHTVVNMFIIPAG
                     MMLGAKVSVIDWIVWNLVPVTLGNLVGGFGFVGLALFVTFARKRPESARTTAMAQPVE
                     S"
     gene            4396..5403
                     /locus_tag="VGC82_17085"
     CDS             4396..5403
                     /locus_tag="VGC82_17085"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0425037.1"
                     /translation="MAGNQQNISTSRRGILVGAPLAGAIGAVPAVASESFWTARYQAS
                     KGGVALAMYRKRLHAPRAGEGRLPVLFLVHGSSISALPSFDLAVPGAGEYSLMNVFAR
                     FGFDVWTMDFEGYGKSTVTPGNSDIASGVADLKAAMSVVERETGQTKFHFMGESSGAI
                     RAGAFAVAEPGRVDRLVLEAFTYTGKGSPTLAKRGQQTEFYRTHNRRSRDKEMILSIF
                     TRDKPGTSDPRVPEAMAKAEMPNGDTVPTGTYLDMTANLPLVDPAKVQAPVLMVRGEY
                     DGISTDEDLLDFFSKLPVPDKQYVVIPGAAHSVAMGYARAGLWHVMHEFLTMPGSNAK
                     T"
     gene            5419..>6260
                     /locus_tag="VGC82_17090"
     CDS             5419..>6260
                     /locus_tag="VGC82_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425038.1"
                     /translation="MGFLSDEERAQLLPAEDVPHRFPIPTQIVSSDEYFPERQTRQQK
                     QVEDRLAEMGDRLAKRQGVSRRRFFQTASGMAAAFLAMNQVYGKIFSVTDAEAATPEM
                     ANERAVTLQGQDVFDAHTHFLRDDTKLTNFVDMRSAVGKAGWNAELGGKPQTIEDLKF
                     DNYFKEVFLDSDTKVALLTNSPSEIPDDWFLTNEMVFQARDRVNQQAGSRRMLAHYTI
                     VPGQPGWLEGIDHAIATLRPDGWKGYTVGDNTHKATSHYPWRLDDEKLMYPAYERFQR
                     AGIK"
BASE COUNT         1190 a         2021 c         2003 g         1046 t
ORIGIN      
        1 ggtcgatcca tcgactgatg tgatacagca tccgcgaccg attggcctaa tcgtactggt
       61 ctcggagggc acgtttacct cgggttgtac ggacgatatt catcaatata tgtctcttaa
      121 ataacgaccc tggcgcgcca acgacacgat ccagtcccgc gcctggtcct cggtcaaacg
      181 gccttccgcc ctggcgatct gccgcagcgc cgcatcgacg tcgcgggcca ttcgggacgc
      241 atcgccacag acgtagaaat gcgcgccgtc ctgcagccag cgccaaagat cggccatctg
      301 ctcgatcatc cggtgctgca cataaacttt gtccttctgg tcgcgtgaga atgcgaggtc
      361 catccggctg agcgtgccgt ccttgatcca cccctcgagc gtcggaccat aaagaaaatc
      421 cgactgccgg tgttggtccc cgaagaacag ccaggtccgc ccgcgagcgc ccgtcgccgc
      481 cctgtcggca aggaatgccc ggaacggcgc gatgccggtg cctggtccga ccatgatgac
      541 cggggtgcgt ggatcggcgg gaagccggaa atggctttgc tgtacactgg ccagcacagg
      601 acccgcgtcc accgcatggc ggccgaggaa tccgctgccc acgccgtgcc gtacccggcc
      661 tcgcctgtcg tcgcggacga cgctgacaca caaatgcacc tgcccctcat gcgcacgcgg
      721 cgaggacgcg attgaataaa gccgtcgccg caacatcggc aacgaccgaa caagatcggc
      781 cagcttcggc cgagcagagg gaaacgcgtg cagaagatcg agcagatcgg gacaatccgg
      841 ctcaatcccg tcatcgccgt cgctgagttt gcgaagcgtt tccgcgtgcg ccggataggt
      901 cgcggtcatt gccagcaggc tcatcgtccg gtccagcggg cgggcgatgt ccagcactgc
      961 gaacgcgtcg cgcagcgggc gatcggtgcc gtcggcacac gtgactcgtt cgtcgccgct
     1021 cgcgcccagt tcggcaatcg tcgcacggat cagcgccgga tcgttacgca ccgcgagact
     1081 gagcgagtcg cccggcagat agctgagacc ggagccggca agatcgatca ccacatgccg
     1141 cacgtccttc gccgaatccg gagccgtcaa ccgttcgctg tgcaggacat ggaccggcat
     1201 accctcgggt tccgctggcg ccttggcaac ctcgggagga accgcgacag cggagggcgc
     1261 ggcggccaga atttgtttca ccatgcgtgc ggtcggcttg gcaccgggca cgcacagcgt
     1321 cgtcgtggtc tcgcggccct cggccagcgc ttcggcgtag gtctggcaca ggtatccgca
     1381 ttgcccgcag tcgagctgcg ccatcgccgc catcagccgg cgcttgaccg gcttgcccgc
     1441 ggccaaagcc aagcgctcgt ccatctccat ggccgggtcg tgccagggga agtcttcctc
     1501 gggttcgact gcggccggcg gcatcggcgt cgtcgcctcg gcgctgccgt acaggccggc
     1561 caggaacccg ttgagccagg agcgctgctc ctcggagaac ggcgcgttgt ccggaatcag
     1621 cgggatcggt gggaggatcg cgttcatgcc gcctccgccg gcacaagatc gataatcgcg
     1681 gccaaggccg cgtcatcgcg ggcggcagtc caggcccgga atgtctcgcc ggcgccgcgt
     1741 tcattcatcc acgatgacaa caaagcgagt atcatcggcg gcagttcggg gaatggaatg
     1801 ccgggacgga tcagccgccc gattgcctga tcggcaccgg cgccgccgcc gacatgaaga
     1861 tcgtagcctt cgaccatgtc ctcgccctgc tcgactttcg cgcccagcag gccgaaatcg
     1921 gcgatgtaat gttgggcgca ggagttgtgg cagcccgtca ggtggatgtt gattggcgcg
     1981 tccacggcga tccgctcctc caggtaatcc accagggccg tggcatggcc tttggtgtcg
     2041 ctggcggcga atttgcagcc ggcattgccg gtgcaggcga ccagtccggt gcgcagccgc
     2101 gacgcctcgg tagatagtcc cagcgcctcg attgctgcca cggctgccgc gacatcggcc
     2161 tcggccacat gcggaatcag caggttctgc caaaccgtca gccggatttc gccgcagccg
     2221 agacaatcgg cgatggccgc cagcccacgc atttgttcgc tggtcagccg cccgaccggg
     2281 catgttatgc caatatagca gcgcccgaac tgtttttgcg gatgcacccc gatatggccc
     2341 agacggtcct gtggtggggc ctgtcggatc gcggcggcat cgacgcgccg caatggttct
     2401 cccagttccg cctcgaccaa ttccaggaag gaaggcatgc caatccggtc gaggacgtat
     2461 ttaagccgag ccttgttgcg gtcggtgcgg tttccctcgg cgatgaagac ccgcagcacg
     2521 gcattggaca cgcggctggt gtcgtccggc ggcacgacca cgccggtttc acgagcgaag
     2581 tcgcggtggc cggtgatccc gcccagcccc aggcggtagt acacgcccgg tgggaccgcg
     2641 gcaccatcct cgaccgtcac cgcggtgaag gcgatgtcgt tggtgtcctc cagcactgcg
     2701 atacgcccgc cgccatcgaa ggcgatgttg aatttgcgcg gcaagccata aagttcctga
     2761 tggtgcagga tgaaatgatg cagcgctcgg gcatgcgggc gggtgtcgat cagctcctgc
     2821 ggatcgatgc cggcggtcgg gctgccggtg atgttgcgga tattgtcggc acccgacccg
     2881 cgcgaggtca ggccgatctc ggcgagccgc atcagcacct cgggcgggcc ggagggaccg
     2941 atttcccgaa gctgcaggtt ggcgcgcgtg gtgatatcgg catacccgcc cgccaggtca
     3001 tcggcgatgt cggcaacccc acggaactgc gcggtcgtca ggatgccgcc aggaatgcgc
     3061 agccggcaca tgaaggcgtt ctgcgccggg gcgacataga acatgccgaa atacttggtc
     3121 aggaagacgt cggttccctt gggaaactga ccgttgctgg cgagagccga catttcatcg
     3181 aaacggcgaa acggaggctt tttacgcttc gcctcttcct ccggcaccag cttgccgccg
     3241 gccgcgatcg tcttgtcctg tgcgatccgc tgcaggtcgc cgggatcggc ggcaaccgct
     3301 ccacccccgg gtgcaaccgg cattccgaag acggcgcgcc gagcctcgac gccggccatg
     3361 aagcctttca ggtactcctg ctgcctgccg gtaaacggct gttccgcagc gctcatgtcc
     3421 tcaagactcc accggctggg ccatcgcggt ggttcgggcg gattccggtc gcttgcgtgc
     3481 gaaggtaacg aacagcgcca aaccgacgaa gccaaagcca ccgacaagat tgcccagcgt
     3541 gaccggcacg aggttccata cgatccagtc aatgactgag accttggcgc ccagcatcat
     3601 gccggcggga atgatgaaca tattgacgac cgtatgttca aagccctggg cgaagaacac
     3661 caggatcggc atccacgccg ccgcgatccg gccgatcacg gaggtggaac agagccccat
     3721 gacgacgccc atgcagacca tccagttgca cagcattgcc ttgacgaaga ccgtcagcat
     3781 gccggctgat ccgaacgccg cgtaaccaat cgtcttggct tccgccagct ggcggactct
     3841 ttcagcgact ccggcgggcg cgatctgcca ggcgtcggtg acggcgataa cgaacagcaa
     3901 gccgtagatg acgctgccga tcaggtttcc cgacagcacc catgaccaat ttacgagcat
     3961 ctgcccaggt tttacctgtc cggccatgac cgccatgggc acaacggcga agctgccggt
     4021 caccagttcc agccccagca gaacgatcat gacgaacccg accggaaaga tcagggcgcc
     4081 gacgagaagc tgcccggttt gcaccgcggc cccaagcgcc aggctggtgg cgattccaag
     4141 caatgcgccg gataacgtgc cgcggaccaa caggtcggcc ggaccgagtt gagcctttgc
     4201 cgttccggac tgtaccatgg cggcgacgat cgcggcgggg gaaacgctat ccatatgtgc
     4261 tcctgcgtct ggcatcacag aagcgagacg caaagcccat gccacgcagg cggccgtgcg
     4321 ggcgctcggg ccctctggac gaggccgcga aatgcgtcct gaataatcgg gacaacaccg
     4381 cgcagaaggt gacatatggc aggaaaccaa cagaatatct cgacgtcgcg gcgcggaatc
     4441 ctggtcgggg cgccgctagc gggtgccatc ggggccgtgc cggccgtggc ctccgaatca
     4501 ttctggacag cgcgctacca ggccagcaag ggcggcgtcg ccttggcgat gtaccgcaag
     4561 cgtctgcatg ctccgcgagc tggcgagggt cggctgccgg tgctgttcct ggtccacggg
     4621 tcatccatct cggcacttcc aagcttcgac ctcgcagtcc cgggggccgg cgaatactcg
     4681 ctgatgaacg tgtttgcccg ctttggcttc gacgtttgga cgatggactt cgagggctat
     4741 ggcaagtcca cggtcacgcc gggcaactcg gacatcgcgt ccggggttgc ggacctgaaa
     4801 gcggcgatgt ccgtcgtcga gcgggaaacc ggccagacga aatttcattt catgggagaa
     4861 agcagcgggg cgatccgagc cggggcgttt gccgtggcag aaccggggcg agtggatcgg
     4921 ctggtgctgg aagcctttac ctataccggc aaaggatctc caacgctcgc caagcggggg
     4981 cagcagaccg agttctaccg cacgcacaat agacgctcgc gtgacaagga gatgattctc
     5041 agcatcttca cgcgcgacaa gcccggcaca tcggacccgc gtgtgccgga agcgatggcg
     5101 aaggccgaga tgccgaacgg cgacaccgtc cccaccggga cgtatctgga catgacggcc
     5161 aatctgccgc tggtcgatcc ggccaaggtg caggcgccgg tgttgatggt ccgcggcgaa
     5221 tacgatggga tttcgaccga cgaggatctg ctcgacttct tcagcaagct accggttccg
     5281 gacaagcagt acgtggtcat tccgggggcg gcgcactcgg tcgccatggg ctacgccagg
     5341 gccggcctct ggcacgtgat gcacgaattc cttaccatgc ctggcagcaa cgccaagaca
     5401 taaccggggg cagcgaccat gggattcctc agcgacgaag aacgcgcgca actactcccg
     5461 gccgaggatg taccgcatcg tttcccgatc ccgacgcaga tcgtctccag cgacgagtac
     5521 tttccggaac gccagacccg gcagcagaaa caggtcgagg accggctggc cgaaatgggc
     5581 gaccgcctgg ccaagcgcca gggcgtcagc cgccgccggt tcttccagac cgcatccggc
     5641 atggcggctg cgttcctggc tatgaaccag gtttacggca agatattctc ggtgaccgac
     5701 gccgaggccg cgacgccgga gatggccaac gaacgcgccg tcaccttgca gggccaggat
     5761 gtgttcgacg cgcataccca cttcctgcgc gacgatacca agctgaccaa cttcgtggac
     5821 atgcgcagcg cggttgggaa agccggctgg aacgccgaac ttggcggcaa gccacagacc
     5881 atcgaggacc tgaagttcga caactacttc aaggaggtgt tcctcgacag cgacaccaag
     5941 gtcgcgttgc tgacgaactc tccgtcagaa attccggacg actggttcct gaccaacgag
     6001 atggtgttcc aggcgcgtga ccgggtgaac cagcaggccg gttcccgtcg gatgctggcg
     6061 cattatacaa tcgtgccggg acagccgggg tggctggaag gcatcgacca cgctattgcg
     6121 acgctgcggc cggacggctg gaagggctac acggtcggcg acaacacgca caaggccacc
     6181 agccactatc catggcggct ggacgacgag aagctgatgt atccggccta cgagcgtttt
     6241 cagcgtgccg gcatcaagaa
//
LOCUS       DASWQN010001001         5762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1943867, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001001 DASWQN010000000
VERSION     DASWQN010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1943867"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VGC82_17095"
     CDS             complement(<1..684)
                     /locus_tag="VGC82_17095"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEY0425039.1"
                     /translation="MKAAVSDFVQTERLAQQMDEHGLDAIVARAGINFTYLSGLVYPG
                     TLARHLDLADSPRGVYLVWPRSGEPRMVVNAIAEGLARRDSFIEHFDVYEGYSEPPVE
                     RLAKVIAGMGLAESKIGFETNFISAADWDVLRSRLPRMQASDSVDLMDMVRAVKTPAE
                     LGRLKRGADLLDEAYLTHFPTVRPGMRERDLHAALVAHCLAGGSEFTHGILNSQRNTV
                     PYAGESDFPF"
     gene            complement(746..2455)
                     /locus_tag="VGC82_17100"
     CDS             complement(746..2455)
                     /locus_tag="VGC82_17100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease/ATP-binding
                     protein"
                     /protein_id="HEY0425040.1"
                     /translation="MWLTMSETGTEIATAARTGGPHRLTLLGGGAALIGMVAAFFGWR
                     SSLIPEPLMEVLTYASSSFLLDGALTAVEIAALAMVGGVMLGLVLALMRLSTLPPIRG
                     TAWLYIWFIRGTPLILQLVFLYDALPVVGIKLDSFTTAVVGFMLNEAAFSAEIIRGGI
                     LSVDRKQSLAAASFGMGPFLTLRRIILPQAMRAILPGMANQTISMIKGTSIASVIFVN
                     ELTFRSQQIVGQNFKFFTVFAAAGIIYLILTSAVAVAQFYLEKFYSLDVSKKPSAKAI
                     PAGEVAAASGSGAPAPSPIAWMDSLQASRTEGSAPDQPFVICRNVQKSYGDKEILRGI
                     DLFVQRGEVVVLMGPSGSGKSTLLRLVNHLEPLDWGEITVDGKYVGYQNQIGGGLKPT
                     RNVAKARADARIGMVFQHFNLFDHLTALENIMEAPIHVYGEAAEKIRALAMNLLRMVG
                     LANHADHLPHRLSGGQQQRVAIARALAISPRLMLFDETTSALDPELVGEVLAVIRRLA
                     EAGMTMIVVTHEVRFAREVADRVIFMDEGHIVEQGTPEDVLDRPRHERTQRFLRMVEQ
                     QVS"
     gene            complement(2446..3777)
                     /locus_tag="VGC82_17105"
     CDS             complement(2446..3777)
                     /locus_tag="VGC82_17105"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0425041.1"
                     /translation="MAGHDGALGQLPAARIGEKTMTTELSGVQKHVDAARVLEVEQAL
                     LRIPSSAFQEQQIADHLAERMKDIGLEVTMMDVVHPFDPTITSRQPMGILRGSGEGPS
                     LMLNGHMDPGVEMPGWSVDPYAAKFEDGWVWGMGAHDDKGGIAAAFCAVEAIIRSGTR
                     LKGDVLLCPVIAHKLGGAGTRALLRSGVRADLCINMEHSNNTIANVCVGIVMVRIRVE
                     APELFFRYSAEAKALYWNPIEQLCEVIRRIGPSLDPIPEGSWMNFISHPDLPGFPTHT
                     FDTFHKEHYYQKNHSGLHSKQAELLLQFRTVPGQTLQSVTADVSNLLEGIRRDHPAFN
                     YSFEVPAKGTEEGWCQEPMACAADHALVVALAAGQELASGAQAIVGGWGRLGNVGDGN
                     IIAAHGIPTVQYGPGDIRIYKEWPTADERVLLSDLVTAARAVAYATVRMCG"
     gene            complement(3809..4693)
                     /locus_tag="VGC82_17110"
     CDS             complement(3809..4693)
                     /locus_tag="VGC82_17110"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0425042.1"
                     /translation="MRNVYKSGLLVSIRGTACLAFLIVSALCGARADDAACEPAKLAV
                     KYPSLVGKTIKIGQDGESVPFSMRDPKDFSKLIGLDADLAQATFACIGVPMQFVIGTW
                     AGLIPAAMSGQIDVMWDTLLYTPERAKKLDFVVYMNAATGMLVAKGNPKNIHAVGDLC
                     ALTATTTLGTTQEAMLRDVSSKCVATGKPAVNIITSTDMPSGLRLVQNARADLAAVNK
                     FVGDSMVAANPTTVESAFDVITGAKIAVGTAKGNPDLVKALRDGLAAIRASGAEKAIY
                     DRYHVDYSLTTEPVTLTE"
     gene            4785..>5762
                     /locus_tag="VGC82_17115"
     CDS             4785..>5762
                     /locus_tag="VGC82_17115"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0425043.1"
                     /translation="MHMHYMGMDSCHLETLLWVVRLGGIGAAAQHLNLTQPAVTRRIQ
                     ELERELGAKVLRRQGRNVALTPVGQTCLGSAERILSEVATMRVAASGKAAVGTIRVGV
                     VESIALTWFQNFLARIEGRYPKVQMEIDVDLSNRLITKLARRQIDIALLPGPVQLPGV
                     VRVSLGDCVMKWLGHPQLCPKDREMRAADLAELPIIGMPQDANAYHSIINWFEKAQVS
                     PSLMHCCNSFSVVALLVRRGVGVSLLPPDLFAGDLDSGVLKVLVEQPYVPKVEYSAAY
                     LPAIDASILPEVAAIAREESWFFGPPRTRSKNFGVGPFPALQVRTILNDE"
BASE COUNT         1165 a         1698 c         1737 g         1162 t
ORIGIN      
        1 aaatggaaaa tcactttctc cggcgtaggg aaccgtgttg cgctgtgagt tgagaatgcc
       61 gtgggtgaat tctgacccac cagccaggca atgcgcgacc agcgcggcgt gcaggtcgcg
      121 ctcgcgcatg ccgggtcgga cggttggaaa atgggtgagg taagcttcat caagaaggtc
      181 ggcgccgcgc ttcaaacggc ccagttcggc gggtgtcttc accgcgcgca ccatgtccat
      241 cagatcaacg gaatccgaag cttgcattcg aggcagacgg ctgcgcagca cgtcccaatc
      301 ggcagcactg atgaagttgg tctcgaagcc gattttcgat tcggccagtc ccatcccggc
      361 gatgaccttg gcaagccgct cgacgggcgg ctcggaatag ccctcataca cgtcaaaatg
      421 ctcgatgaat gaatcacgcc gcgcaagccc ttcggcgatt gcattgacca ccatgcgtgg
      481 ttcacccgag cgcggccaaa cgagatagac gcctcgcggc gagtcggcga ggtctagatg
      541 gcgcgccagc gtacccggat agacgagacc agaaagataa gtaaagttga ttccggcccg
      601 cgcgacgatc gcatcaaggc catgttcatc catttgttgg gcgaggcgct cggtctgcac
      661 aaaatcggaa accgctgctt tcatgtttgt caactttcct gaagactttt taggggcgct
      721 cacggctccg cgggcgcggt gagatttagc taacttgctg ttcgaccatc ctgaggaaac
      781 gctgggtgcg ctcgtgtctg ggacggtcca gcacgtcttc gggtgtgcct tgttcgacga
      841 tatgaccttc gtccatgaag atcacgcgat cagcgacctc gcgcgcaaac cgcacctcat
      901 gggtcacgac gatcatggtc atccctgctt cggcgaggcg gcggatcacg gcaaggacct
      961 ctcctaccaa ttcggggtca agcgccgaag tggtctcgtc gaaaagcatc aggcgaggcg
     1021 agatggcgag agcacgggcg atcgccacgc gctgctgctg gccgcctgac agccggtgcg
     1081 ggagatggtc ggcatggttg gcgagaccca ccatgcgcaa caaattcatc gcgagcgcgc
     1141 gtatcttctc ggctgcttcg ccataaacat ggatcggcgc ttccatgata ttctccagcg
     1201 ccgttaagtg atcgaacaaa ttgaaatgct ggaacaccat accgatgcgc gcatcggcac
     1261 gggccttggc aacgttccgg gtaggcttca atccgccccc gatctggttc tggtatccga
     1321 catatttgcc atcgaccgtg atctcgcccc agtccagcgg ctccagatgg ttgaccagcc
     1381 gcagcaacgt gctcttgcct gagccgctcg gtcccatcag cacgacaact tcgccgcgtt
     1441 gaacgaacag atcgatgccg cgaaggattt ccttgtcgcc atatgatttt tggacgtttc
     1501 ggcagatcac gaagggctga tcgggggccg aaccttccgt acggcttgcc tggagactgt
     1561 ccatccaggc gatcggtgac ggtgcaggcg cgcccgaacc gctcgccgcg gcaacttcgc
     1621 cggccggaat ggctttcgcg ctcggcttct tcgaaacatc gaggctgtaa aatttctcca
     1681 ggtagaattg cgccaccgcc accgcgctgg tcaggatgag gtagatgatg ccggcagcgg
     1741 caaataccgt gaaaaacttg aaattctggc cgacgatctg ctgggagcgg aatgtgagtt
     1801 cgttgacgaa gatcaccgat gcgatcgacg tccccttgat catggaaatg gtctggttgg
     1861 ccatgccggg cagaatggcc cgcatggcct gaggcagaat gattcgccga agcgtcaaaa
     1921 acggtcccat gccgaaggaa gctgcggcga ggctttgctt gcgatccacc gaaagaatgc
     1981 cgccgcggat gatctcagcg ctgaacgcgg cctcgttcag catgaaaccg acaacggccg
     2041 tcgtgaaact atcgagcttg atcccgacaa ccggcaacgc gtcgtagaga aagaccagtt
     2101 gcaggatgag gggagtgccg cggatgaacc agatgtagag ccaggccgtt ccacgtatcg
     2161 gcggcagtgt ggagaggcgc atcaatgcca gcaccagacc gagcatgacg ccacccacca
     2221 tagccagcgc ggcgatctcc acggcggtca gagcaccgtc gagcagaaag gaggaagagg
     2281 cgtaggtgag gacctccatg agcggctcag gtatcaggct ggaacgccag ccaaagaaag
     2341 cggcgaccat gccgatgaga gctgcgccac cgcccaacaa ggtcagtcta tgcggcccgc
     2401 cagtgcgtgc cgcagtcgcg atttccgtcc ctgtttcgct catggtcagc cacacattct
     2461 gacggttgca tatgccaccg cgcgcgcggc ggtgacgaga tcggagagca gcacgcgctc
     2521 atcggcggtg ggccattcct tgtagatgcg aatgtcaccg ggaccatact gcacggtcgg
     2581 aataccatgg gcggcgataa tattgccgtc gccgacattg ccgagccgtc cccaaccgcc
     2641 aacgatggcc tgggcgcccg atgccaattc ctggcccgct gccagtgcga ccaccaaggc
     2701 atggtccgca gcgcaagcca tcggctcctg gcaccatcct tcctcggtgc ccttggccgg
     2761 cacctcgaag ctgtagttga aagccgggtg atctcgccgg atgccttcca gcaggttcga
     2821 aacatccgcg gtcacgcttt gcagcgtttg tcccggtacg gtgcggaact gcagcagcag
     2881 ctcggcctgc ttgctatgca ggccgctatg gttcttctgg tagtaatgct ccttgtggaa
     2941 agtatcgaag gtgtgagtcg gaaagcctgg caggtccggg tgagagatga aattcatcca
     3001 acttccttcc ggaatgggat cgaggcttgg cccgatgcgg cgaatgacct cgcaaagctg
     3061 ctcgatcgga ttccagtaca aggcctttgc ttcggcgctg tagcggaaga acagctccgg
     3121 agcctctacc cggatgcgca ccatcacgat gccgacgcag acattggcga tggtgttgtt
     3181 ggaatgctcc atgttgatgc acaaatccgc ccgcacgcca ctacgcagca gcgcccgggt
     3241 gccggcaccg ccgagcttgt gtgcgatcac cggacacaac aacacgtcgc ctttgagccg
     3301 cgtgcctgag cgaatgatgg cctccaccgc gcagaacgca gcagcgatgc cgcctttgtc
     3361 gtcgtgggcg cccatacccc aaacccagcc gtcctcgaat ttggcggcat aggggtctac
     3421 cgaccatccc ggcatttcga cgcccggatc catgtgcccg ttaagcatca ggctcggacc
     3481 ttcaccgctg ccgcgcaaaa tgcccattgg ctgccggctg gtaatcgtgg gatcgaacgg
     3541 atgcacgaca tccatcatgg tgacttcgag gccgatgtcc ttcatccgtt ctgcgagatg
     3601 atcggcaatc tgttgctcct ggaaggcgga actggggatg cggagcaacg cttgctcgac
     3661 ttcgagcacg cgcgcggcat cgacgtgctt ctgcacgccc gacagttcgg tagtcatggt
     3721 cttttctccg atacgcgccg ccggcaattg gcccagtgcg ccgtcatgac cggccatcaa
     3781 gtgttcggcg cgatggcctg gattcggctc actcggtcaa ggtcacaggc tccgtggtga
     3841 ggctatagtc cacgtggtag cggtcataga tcgccttctc cgctccgctg gcgcgtatgg
     3901 ccgcgaggcc gtcacgcagc gccttgacca gatcggggtt gccctttgcc gtgcctaccg
     3961 caatcttggc accggtgatc acgtcaaacg cgctctcgac cgtggtgggg ttggcggcca
     4021 ccatgctgtc gccaacgaat ttgttgaccg cggctagatc cgcacgcgca ttctgcacga
     4081 ggcgcaagcc gctcggcatg tcggtggagg tgatgatatt gaccgcgggc ttgcccgtgg
     4141 cgacgcactt gctgctgacg tcgcgcagca tggcctcctg cgtagtaccg agcgttgttg
     4201 tagcggtgag agcgcacagg tcaccgacgg catggatgtt ctttggattg cctttggcga
     4261 cgagcatccc ggtagcagcg ttcatataga ccacgaaatc cagtttcttg gcccgctcgg
     4321 gtgtgtacag cagggtgtcc cacatgacgt caatctgacc tgacatcgca gccggaatga
     4381 gcccggccca tgttccgatc acgaactgca tgggcacgcc aatgcaggca aaggtcgctt
     4441 gggccagatc ggcatccagt ccgatcagct tcgaaaaatc cttggggtcg cgcatgctga
     4501 acggcacgct ctcaccatcc tggccgatct tgatcgtttt gccgacgagg ctgggatact
     4561 tgactgcaag ctttgccggt tcgcacgcag catcgtctgc tcttgcgccg cacaatgcgg
     4621 aaacgatcag gaaagccaga caggcagtgc cccgtatgga aaccagaagc ccggacttat
     4681 aaacattccg catgaattct tttccacttt ttagagacga cccggcgaat gcagattatt
     4741 cgttgagatt ggaccaatac aaatgaatta tagacattca attcatgcat atgcattata
     4801 tgggaatgga ttcttgccat ctggaaacgt tattatgggt agtcaggctt ggtgggattg
     4861 gcgctgcagc gcagcatctt aatcttactc agccggccgt cacccgccga attcaagagc
     4921 ttgagcggga gctcggcgcc aaggtgctac gccgccaagg gcggaatgtg gcgctcacac
     4981 cggttgggca gacctgcctt ggcagcgccg agcgcatcct gtcggaagtt gccaccatgc
     5041 gcgtcgcagc gagcggcaaa gcggctgtcg gcaccatccg cgtcggggtg gtcgaaagca
     5101 ttgcgctgac gtggtttcaa aatttcctcg cccgaatcga gggacgctat cccaaggtac
     5161 agatggagat cgatgtcgat ttgtcgaacc ggttgattac caagcttgct cgccgtcaga
     5221 tcgacatcgc gttgctgccc ggtccggtgc agctgccagg ggttgtgcgt gtgtctctcg
     5281 gcgattgcgt catgaagtgg ttgggccatc cgcaattgtg cccgaaggat cgtgaaatgc
     5341 gggccgcaga cctcgccgag ttgccgatca tcggcatgcc gcaggatgcc aatgcgtatc
     5401 attccatcat caactggttc gaaaaggctc aggtcagccc tagtctcatg cattgttgca
     5461 acagcttcag cgtggtggct ctgctggtac ggcggggtgt aggggtcagc ttgctgccgc
     5521 cggacctatt cgctggcgat ctcgactcgg gcgtcttgaa ggtactcgtc gaacaaccct
     5581 acgttccgaa ggtggaatat tcagctgcct atttgcccgc catagacgcc tcgatccttc
     5641 cggaagtagc ggcgatcgcg cgggaggaaa gctggttctt cggcccgccg cgaacacgaa
     5701 gcaagaattt tggcgtgggg cctttccctg cgctgcaagt ccggacgatc ttgaacgatg
     5761 ag
//
LOCUS       DASWQN010001002         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_228772, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001002 DASWQN010000000
VERSION     DASWQN010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228772"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..>1730
                     /locus_tag="VGC82_17120"
     CDS             347..>1730
                     /locus_tag="VGC82_17120"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0425044.1"
                     /translation="MDTLTQPLLRQFDDFLLDLRIRVLFRLNGGSAPEPVPLGSRACD
                     VLCLLVEHHGAIVSRQAVIDKVWPNVAVEENNLTVQISALRRVLDEGRARGSCIQTIP
                     GRGYRFLPDVVKTEHPPAGDVAASVGPTILQYPRDDHADESVTIKSRPPLSAGARDTY
                     SPMVRWRWLRIVAWIVAATCVAFGAVLGTQTWRAFHDTVRHPRLSVAVLPFQNLGGNP
                     AEEYLADAITDDLTTDLSHIPEAFVIARESAYTYKGKATDVRQIGHELSVRYLLEGSV
                     RKLGTTLRVNVQLISAETGLHLWSDRFDQDVNDLAEGQQRVVARMSGALGISMVDIER
                     TRSLRERPTNPDAFDLILQARALRNQPETRQRTDEALALYERALALDPSSVLAMAGIG
                     YLLPQQRIARTFWMDLEAMERAKNLTAQALAIAPMSEDALKPQMTTCLRTILSRFQMI
                     RNMNIEIGQRG"
BASE COUNT          347 a          525 c          505 g          353 t
ORIGIN      
        1 aagttcagcg tccaggaatg acttcacccg cacgctgaag ccagaatcct ctggcgaaac
       61 aaataagtgc tgcgatcact aagcccgcta ctcatcgctg tacgaagcat tgttcagcca
      121 tccgctaccg cctcgccgca actgtcattg cagcgggtcg cgaaatgcgc gatgcggtta
      181 aggttaattc ttgtgaatcg atctaaatca tgtcggctgg accgcgccaa caaagcaatt
      241 gcggccggtg tttgccgatc ttatcatgcc aaagagcaaa tccagtcgtt cacgctcgcg
      301 ccgatacttc tgcattcatc ctggcaagat catacgggag gtggccatgg acaccctgac
      361 ccaacccctc ctccgccaat tcgatgattt cctgttggat ttacgaatcc gggtgttgtt
      421 tcgattgaac ggtggaagtg cgccggagcc ggttccgctc ggatcgcgcg cttgcgacgt
      481 actctgcctt ttagtcgagc accacggtgc gatcgtttcc aggcaggctg ttatcgataa
      541 ggtttggccg aacgtcgccg tggaggaaaa caacctgacg gttcagattt cggcactgcg
      601 gcgtgtgctg gacgagggac gtgcacgggg aagctgcatc cagaccattc ctggccgagg
      661 ctatcgtttc ctgcccgacg tcgtgaagac ggaacaccct ccggctggcg acgtggccgc
      721 aagtgttggt ccgactattc ttcaatatcc gcgcgacgac cacgccgatg aatcagtcac
      781 catcaaatca cgtccgccgc tgtccgcggg cgcacgcgac acgtattcgc cgatggtccg
      841 ttggcggtgg ctgaggattg tcgcctggat cgtcgccgcg acctgcgtgg ccttcggcgc
      901 ggttctgggg acgcagacct ggcgcgcctt ccatgacacc gtgaggcatc cccgtttatc
      961 tgtcgctgtc ctgccattcc agaaccttgg cggcaacccg gccgaggagt acctggctga
     1021 cgccattacc gacgacctga ccacggatct gtcccatatc cccgaagcat tcgtgatcgc
     1081 ccgcgaatcc gcctatacgt acaagggaaa agccaccgat gtccgccaga tcggccatga
     1141 actaagcgtg cgctacttgc tggagggcag cgtccgcaag ctcggcacca cgctgcgggt
     1201 gaatgtccag ttgatatcgg ccgagactgg gcttcacctc tggtcggacc gctttgacca
     1261 ggatgtcaat gacctggcgg aagggcagca gcgtgtggtt gcgcggatgt cgggtgccct
     1321 gggcatcagc atggtcgata tcgagcgcac gcgcagcctg cgcgagcgac cgaccaatcc
     1381 tgatgccttc gatctgatct tgcaggcgcg ggcactgcga aaccagcccg agacgcggca
     1441 gcgcaccgac gaggccttgg cgctatacga gcgtgcgctg gcgctcgatc caagttctgt
     1501 cctcgccatg gccggcattg gctatttgct gccccagcaa cgcatagctc gcaccttctg
     1561 gatggatctt gaggcgatgg agcgggcgaa aaacctgacg gcccaggcgc tggcgatcgc
     1621 accgatgtcg gaggacgcgc ttaagcctca aatgacaacc tgcttacgca ctatcctcag
     1681 ccgatttcag atgatacgga acatgaacat cgagattgga cagaggggct
//
LOCUS       DASWQN010001003         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2604662, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001003 DASWQN010000000
VERSION     DASWQN010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2604662"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1635
                     /locus_tag="VGC82_17125"
     CDS             <1..>1635
                     /locus_tag="VGC82_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0425045.1"
                     /translation="KTSRNPLMRFQHGFERLFTKLRDGYLGLLRLCLQHRIKLIAGFL
                     GLTLLSFGLAPFLGQDFFPAVDGGQIKLHIRAPTGTRIEETTSLTDRIGTAIHGVIPA
                     SELDGIVSNIGLTVSGINMAYNNSGTIGVGDADILISLKPNHAPTDDYIKTMRVKLPQ
                     QFPGTSFAFLPADIVSQVLNFGVPAPIDLQVAGRDLAADRTYANSLLAKIREIPGIAD
                     ARIQQAFQQPTLDVNVDRSLTSLVGLTEKDVATAMLTTLSGSSQSAPTYWLNPANGVS
                     YAVSVQTPQRDINTMTGLKNIPVSSATANPQLLGGLAQVQRTNSNAVVSHYDVQPVVD
                     IFATPQGRDLGAVAADIQQVIHATAKDLPKGASVALRGQVSTMTSAYRQLYVGLAAAI
                     VLIYLLIVINFQSWIDPFVIVMALPTALAGVVWMLFATGTTLSVPALTGAIMCMGVAT
                     ANSILVISFARERMEAGLDGVAAAFEAGGSRFRPVLMTALAMVIGMLPMAIEPGQNAP
                     LGRAVIGGLIFATCATLFLVPTIFALVHVRQRSPAAA"
BASE COUNT          272 a          540 c          515 g          308 t
ORIGIN      
        1 aaaaccagcc gcaatccgct gatgcggttc cagcacggct tcgaacgttt gttcaccaag
       61 ctccgcgacg gctatctggg cctgctgcgg ctctgcctgc agcaccggat caagctgatc
      121 gcgggattcc tcgggttgac cctcctgtcg ttcgggcttg cgccgtttct cggccaggac
      181 tttttcccgg cggtggatgg cggccagatc aagcttcata tccgtgcgcc gacgggtacg
      241 cggatcgagg agacgaccag cctgaccgac cggatcggca ccgccatcca cggcgtcatt
      301 ccggccagtg aactcgacgg catcgtgagc aacatcgggc tcacggtcag cggcatcaac
      361 atggcgtaca ataattccgg caccatcggg gtcggagacg ccgacatcct gatcagtctc
      421 aagcccaatc acgcgccgac cgatgactac atcaagacga tgcgggtcaa gctgccgcag
      481 cagttccccg gcaccagctt cgccttcctg cctgcggaca tcgtcagcca ggtattgaat
      541 ttcggcgtgc ccgcgcccat cgacctgcag gttgcgggca gggaccttgc cgcggatcgg
      601 acctatgcga attcgctgct ggccaaaatc cgcgagatcc caggcattgc cgacgcgcgc
      661 atccagcagg cgtttcagca gccgacgctt gacgtcaacg tcgatcgctc gctgacctcg
      721 ctggtcggcc tgaccgagaa ggacgtcgcc accgcgatgc tgacgacgct gtccggaagc
      781 tcgcagtcgg ccccgaccta ttggttgaat cccgccaacg gcgtgtctta cgcggtctcg
      841 gttcagaccc cgcagcgcga catcaacacc atgaccggct tgaaaaacat cccggtgtcg
      901 tccgctaccg cgaatcccca gttgctcggc ggcctggcgc aggtccagcg gacgaacagc
      961 aacgcggtgg tgtcgcatta tgatgtgcag cccgtggtcg acattttcgc gacgccccag
     1021 ggtcgcgatc tcggtgcggt tgccgcggac attcaacagg tgatccacgc caccgccaaa
     1081 gacctgccca agggcgcgag cgttgcgctg cgcgggcagg taagcacgat gaccagcgcc
     1141 tatcggcagc tctatgtcgg cctggcggcg gcgatcgtgc tgatctacct cctgatcgtt
     1201 atcaatttcc aatcctggat cgatccgttc gtcatcgtga tggcgctccc gacggcgttg
     1261 gcgggcgtcg tctggatgct gttcgcaacg gggacgacgc tttcggtgcc cgcgctcacc
     1321 ggcgccatca tgtgcatggg ggtggcgacc gccaacagca tcctggtgat cagctttgcc
     1381 cgcgagcgca tggaagctgg cctggatgga gtggcggcgg cgttcgaggc gggcggctcg
     1441 cggttccgcc cggtgctgat gacggcgctc gccatggtga tcggcatgtt gccgatggcg
     1501 atcgagcccg ggcagaacgc gccgctgggg cgcgcggtga tcgggggcct gatcttcgcc
     1561 acctgcgcca cgctgttttt ggtgccgacg atcttcgctc tggtgcatgt ccggcagcgc
     1621 agccccgctg ccgct
//
LOCUS       DASWQN010001004         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_228911, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001004 DASWQN010000000
VERSION     DASWQN010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228911"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VGC82_17130"
     CDS             <1..393
                     /locus_tag="VGC82_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425046.1"
                     /translation="PATRSLINSVIRERCTSLFASRWDRTGATCRGGTGQTPPLQAPS
                     LGDTKKATALTGIAGCCCAIMPRPQRQDPRDKVDERAVSWQLFYGGRTDKDCLEIHEQ
                     MIGVVKLKQPDPLDENAKAQGFCHAFAR"
     gene            complement(395..1264)
                     /locus_tag="VGC82_17135"
     CDS             complement(395..1264)
                     /locus_tag="VGC82_17135"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEY0425047.1"
                     /translation="MVLADGTLLTACAEKHPDLFWALRGGGGNFGIAASMEYRLHPVG
                     PMVTGGVIAYPFHEAWDVLRFYRDVTSSLPDEFIVFGGLIHAPDGSGAKLVAMIVCHC
                     GSLADGEDALKTIKAFRSPAMDAAGPIPYCQLNMMLDDGFPRGALNYWKSAFLSGLGD
                     DAIRTMIDSFAQCPTPMGALMLEHFHGAVTRVGVTDTAFPHRDEGHNLLVLGQWTDPK
                     DNEACISWARDAYNAMAAFMGPGRYVNYLGEDEQSEVPSAYGQNYRRLTQIKAKYDPD
                     NFFRMNQNIRPSA"
     gene            1298..>1702
                     /locus_tag="VGC82_17140"
     CDS             1298..>1702
                     /locus_tag="VGC82_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425048.1"
                     /translation="MLSHKPTDAAAEREATDTGRGNHAPGSRETMELRFPVESVPCES
                     ALSAGSSDFGINMHSLHRREVYHYATVYCRTASDVVTSSAHSDFEVPLARQSYRIDDV
                     SRTAALRDDSRSLVDQAVVDSSGGLVGWVGWLK"
BASE COUNT          349 a          505 c          517 g          331 t
ORIGIN      
        1 ccagccactc gcagcctgat caattcggta attcgagaac ggtgtactag cctcttcgca
       61 agccggtggg accgtacagg agccacctgt cgcggtggaa ccggccagac gccgccgctc
      121 caagctccat cgctgggcga cactaagaag gcaacagcgc tgacggggat tgccgggtgc
      181 tgctgcgcta ttatgccgcg acctcagcgt caggatccac gggacaaggt agatgagcgt
      241 gccgtctcct ggcagctgtt ctacgggggc cgaactgaca aggactgcct ggaaattcat
      301 gagcagatga ttggcgtggt caagctgaag cagccggacc ccttggatga gaacgcgaaa
      361 gcgcaaggct tttgccacgc ctttgcccgg tgaattacgc ggaaggccga atattctggt
      421 tcattcggaa gaagttgtct ggatcgtact tcgccttgat ctgggtgagt ctccggtagt
      481 tctggccata ggccgaggga acttcactct gctcatcctc gcccaaatag ttgacgtagc
      541 ggcctggccc catgaacgcg gccatggcgt tatacgcatc ccgcgcccag gagatgcagg
      601 cttcgttgtc tttcggatca gtccactgac ctagaacaag caggttatga ccttcatccc
      661 ggtgcgggaa tgccgtgtcc gtgacgccaa cacgggtgac ggcgccatga aagtgttcaa
      721 gcatcaaggc acccatgggc gttggacatt gtgcgaagct gtcaatcatc gtgcggatcg
      781 cgtcgtcgcc gaggccagaa aggaaggctg atttccagta attgagcgcg ccgcgcggaa
      841 acccgtcgtc caacatcatg ttcaattggc agtaaggtat aggacccgcg gcgtccatcg
      901 ccggcgagcg gaacgccttg atcgtcttca gcgcgtcctc gccgtccgcg agtgagccac
      961 agtggcacac gatcatagcg accagcttcg cgcccgaacc atctggggcg tggatgagcc
     1021 cgccgaaaac tataaactcg tcaggcaacg acgaggtgac gtcgcgatag aagcgcagaa
     1081 cgtcccaggc ttcgtggaac gggtatgcga tcaccccgcc ggtgaccatg ggaccaactg
     1141 gatgcaggcg atactccatg gacgcagcga tgccaaaatt cccgccaccc ccacgtaggg
     1201 cccaaaacag gtctgggtgt ttttccgcgc aggcggtgag gagtgtgccg tccgccagga
     1261 ccacatcgac tgaaagcaga ttgtcgagag caagggcgtg ctttcccata agccaaccga
     1321 tgccgccgcc gagcgtgagg ccaccgatac cggtcgtgga aatcacgccc ccggtagtcg
     1381 cgagaccatg gagctgcgtt tcccggttga atccgttcca tgtgagtccg ccctgagcgc
     1441 gggcagttcg gactttggga tcaacatgca ctccctgcat cgtcgagagg tctatcatta
     1501 cgccaccgtc tattgtcgaa cggccagcga cgttgtgacc tcctccgcgc acagcgactt
     1561 cgaggttccg ctcgcgcgcc agtcttatcg catcgacgat gtcagccgca ccgcggcact
     1621 gcgcgatgat agcaggtcgc ttgtcgatca ggccgttgtg gactcttctg gcggtctcgt
     1681 aggttgggtc ggctggctga ag
//
LOCUS       DASWQN010001005         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2471209, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001005 DASWQN010000000
VERSION     DASWQN010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2471209"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..1365)
                     /locus_tag="VGC82_17145"
     CDS             complement(334..1365)
                     /locus_tag="VGC82_17145"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0425049.1"
                     /translation="MKDFSIGVDLGGTNLRIAAVDVSGHLVEKVTLGTKVSLGRDHVL
                     NDMCDAIQHLSDKYRNSSKMLGIGIGVPGIIDMETGLLRESPNLPGWADYPVRAEIER
                     RLKTRVILENDANVAAFGEKWLGAAKEHDDMAMLTLGTGVGGGLVIGGQIWHGMNGMA
                     GEFGHTTVEPEGHPCGCGNRGCLEQYASATAVVRMAREAIAENQGCSLAKAAHSDAEF
                     SAKSIFNFALQGDEDAKRIYRRVGRALGIVLSTLVNSLNLPMYVVGGGVASAWEVFAP
                     TIFAELRSRCMVYAATAPPDPLGQGGGASAHLEPGPGHKTIITRALLGSDAGLFGAAR
                     LPMVPEAKR"
     gene            complement(1465..>1771)
                     /locus_tag="VGC82_17150"
     CDS             complement(1465..>1771)
                     /locus_tag="VGC82_17150"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEY0425050.1"
                     /translation="NIYADESLFRTGIRPRRRASTLTREQLRRLHHSIREVLSEAIAL
                     GGSSISDYVDADGEEGFFQLQHRVYGREGEPCLVCRTHVKRIVLTGRSSHYCPKCQK"
BASE COUNT          356 a          529 c          513 g          373 t
ORIGIN      
        1 accttcagcg ccattccgtg gccgacctga ccgtgctcct gaaggtcgaa gcggtgatcg
       61 tgcaactggc tcgggctccc gttcgggctc ttcaaaggca ttcgaacgac cttcagcgcc
      121 ttctcggggt cctcgaccac cattcaagaa gggaaagtct ttcggcaggc ccaaagatgg
      181 accgggttcc ggcggaccga agacgggcac ggggcgtcca ttcagcaaga gcggaccggg
      241 atttgaagga cggcgttctg ggaccgacgc aactgggaaa ccgccctcat catcatcatc
      301 atcgtcatca tcacgtcccc gagggccgcg acgttaacgc ttcgcctccg gaaccattgg
      361 caggcgggct gcgccaaata atcctgcatc gcttccgagc aatgcgcgcg tgatgatggt
      421 tttgtggcct gggcccggct cgaggtgtgc ggacgcccct ccgccttgtc ctagtggatc
      481 gggaggagcg gtggctgcgt agaccatgca gcggctgcgc agttccgcga aaatcgtagg
      541 cgcaaacact tcccatgcgc tggcaactcc gccgcccaca acgtacatgg gcaggttcag
      601 cgaattcacc agcgtggaga gcacgatccc cagcgcgcgg cccacgcggc ggtaaatacg
      661 cttcgcgtct tcatcacctt gcagcgcaaa attgaaaatg gatttggcgc tgaattccgc
      721 gtctgaatgc gcggcttttg ccagtgagca gccctggttt tcagcgatgg cttcgcgcgc
      781 catgcgcacg accgcggtgg ccgatgcgta ctgctcaagg catccgcgat taccgcagcc
      841 gcagggatgg ccttctggct cgacggtggt gtgtccaaat tcgccggcca ttccgttcat
      901 gccatgccag atttgtccgc cgatcacgag gccgccgccc actccagtgc cgagagtcag
      961 catcgccata tcgtcgtgct ctttggcggc acccagccac ttctcgccga atgccgcaac
     1021 attggcatcg ttctcgagaa ttacacgggt cttgagccga cgttcgattt ccgcgcgcac
     1081 cggatagtcc gcccagccgg gtaggttcgg agactcgcgc agcagtccgg tctccatatc
     1141 gatgatgccg ggcactccga tgccgatacc aagcatcttg gaggagtttc tgtacttatc
     1201 ggaaagatgc tggatggcgt cacacatgtc gttcagaaca tggtcgcggc caagagaaac
     1261 tttagtgcct agcgtgactt tttcgaccag gtggccgctc acatcgacgg cagcgatacg
     1321 aaggttggtg ccgcccagat cgactccaat ggagaaatct ttcatataag gaaactctat
     1381 tttagcctgt agatgggagg aggcggcctt ctgttttgta catcggcgct ttccgatcgt
     1441 caatattaaa tcgttatact accgctattt ttggcatttt ggacagtagt gactgctccg
     1501 gcctgtcaac acgatacgtt tgacatgagt gcggcacacc aaacacggct caccctcgcg
     1561 gccgtacacc cggtgctgaa gctggaagaa tccctcttca ccgtctgcgt caacgtagtc
     1621 tgaaatcgat gagccgccca gtgcaatggc ttcgcttaga acctctcgga tggaatgatg
     1681 gagtcggcga agctgctccc gagtcagggt cgaagcgcgg cgacgcgggc gaatgccggt
     1741 acggaacagc gattcatccg catagatgtt c
//
LOCUS       DASWQN010001006         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_755114, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001006 DASWQN010000000
VERSION     DASWQN010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755114"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1648)
                     /gene="pepN"
                     /locus_tag="VGC82_17155"
     CDS             complement(<1..>1648)
                     /gene="pepN"
                     /locus_tag="VGC82_17155"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY0425051.1"
                     /translation="AAVSDFNMGAMENKGLNVFNTKYVLARPETATDSDYQGIETVIA
                     HEYFHNWTGNRVTCRDWFQLSLKEGLTVYRDQEFSADQGSRAVKRIGDVRGLRAAQFR
                     EDAGPLAHPVQPASYMAIDNFYTATVYNKGAEVIRMMATIIGRAAFRRGMDLYFERHD
                     NNAVTIDDFAQAMQDASGVDLTDFKLWYHQAGTPEVSLEDAHDPAAKRYSLTIRQTTK
                     PTRGQPEKSPLVIPVAMGLLDGNGQELATQLEGEPEPVAGTRVLLAVHAENRFEFINV
                     ASPPIPSLLRDFSAPVKLSGLSPDRLRFLAAHDTDPFVRWDSGQQFAAMTLLDMVAAI
                     QAGDEPAVDPALIEAAEAALTQEPALAAEALALPGEANLADKMEVVDADAIHAARDMA
                     RAAIGQALRDRLRAMYDDLTDSGGYTIDGPAIGRRSLRNMCLSYLVAGGDASAVRLAK
                     AQFDLGQNMTDVLAALGILSGVDCVERVDALAAFYRTWRGDPLVLDKWFAIQALSPLP
                     NTLREVETLKTHPDFDIRNPNRVRALISGFAGNQVRFHDPS"
BASE COUNT          303 a          537 c          499 g          309 t
ORIGIN      
        1 ccgatggatc gtggaacctt acctggttgc cggcgaaacc gctgatcagc gcgcgcacac
       61 ggttcgggtt ccggatatcg aagtccggat gagtcttcag ggtttccacc tcccgcaacg
      121 tattcggcaa cggcgacagc gcctggatcg cgaaccactt gtccaacacc agggggtcgc
      181 cgcgccatgt acggtagaac gccgccagcg catccactcg ttccacacag tccacacctg
      241 aaagaatccc gagcgccgcc agaacgtcgg tcatgttctg gccaagatcg aactgcgcct
      301 tggcgaggcg gacagcggac gcatcgccac cggcgaccag gtagctcaga cacatattgc
      361 gcaacgaccg tcgcccgatc gccggcccgt cgatcgtgta gccaccgctg tccgtcaggt
      421 cgtcatacat cgcgcgcagg cgatcccgca gcgcctggcc gatcgccgct cgggccatgt
      481 cgcgtgcggc gtgtatcgcg tcggcgtcaa cgacctccat cttgtcggcc aggtttgctt
      541 cccccggcaa tgccaaggcc tcggccgcca atgccggttc ttgcgtcagc gcggcttcag
      601 ccgcttcgat cagcgccgga tcgaccgctg gctcgtcacc ggcctggatg gcggcaacca
      661 tgtccagcag cgtcatcgcg gcaaactgtt gacccgagtc ccagcggaca aacggatcgg
      721 tgtcgtgcgc cgccaggaaa cgcaaccggt ccggcgacag gccagataat ttcaccggcg
      781 ccgaaaaatc ccgcagcagc gacggtatcg gggggctggc gacattgata aactcgaaac
      841 ggttttccgc atggacggcc agcagcacgc gcgtgcccgc caccggctcc ggctctcctt
      901 ccaactgggt cgcgagttcc tggccgtttc cgtccagcaa tcccattgcg acaggaataa
      961 ccaagggcga tttttctggc tgcccccgcg tgggtttggt ggtctgccgg atggtcagag
     1021 aataacgctt tgcagcaggg tcgtgggcat cctcgagcga aacctccggc gtgccggcct
     1081 gatgatacca cagcttgaag tcggtcagat cgacgcccga cgcatcctgc atcgcctgag
     1141 cgaaatcatc gatcgtgacc gcgttgttgt catgacgctc gaaataaagg tccatgccgc
     1201 ggcggaacgc cgctcggccg atgatggtgg ccatcatgcg gatgacttcg gcacccttgt
     1261 tgtaaaccgt cgcggtatag aagttgtcga tcgccatgta gctggccggc tgcacgggat
     1321 gggccagggg gccggcgtcc tcgcgaaact gcgccgcccg caaacccctt acatccccaa
     1381 tccgtttgac cgcgcggctg ccctggtcgg cggaaaattc ctggtcgcga taaaccgtca
     1441 ggccttcctt gagggaaagc tggaaccagt cgcggcaggt cacgcggttg ccggtccagt
     1501 tgtggaaata ctcgtgcgcg atgacggttt cgatcccctg gtaatcactg tcggtcgcgg
     1561 tttccggacg cgccagaacg tatttcgtgt tgaagacgtt caggcccttg ttctccatcg
     1621 cgcccatgtt gaagtcggac acggcggc
//
LOCUS       DASWQN010001007         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_624741, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001007 DASWQN010000000
VERSION     DASWQN010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624741"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1220)
                     /locus_tag="VGC82_17160"
     CDS             complement(150..1220)
                     /locus_tag="VGC82_17160"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0425052.1"
                     /translation="MQFGIAIPTAADSWRLVRRAEELGFSHAWFFDTQMLSADPFVAM
                     AASAMKTSRIKLGTGVLIPSNRIAPVAATAFASLNKLAPGRVIFGVSTGFTGRRTMGL
                     GAVKLADMEQYIRVIQALWRGETVEFAVEGRLRKIRLLNPELGLINTDDPMPTYIAAS
                     GPKARALTAKLAAGWIDNVADAERGAATLEQMRIAWQDAGHKREALTSVAWIGGAVLE
                     DGEPVDGPRGMALAAPRAAMLLHRAADTALAGYPSPVAMRPEFADAVEAYVEHARGFE
                     PKDAYYLSNHRGHLMFVRPDERRFITAEMIRRTSFIGTAADLTQRIGALADAGFDQVV
                     FSIPPGQEHAIEDWGRIRRAFG"
     gene            1420..>1629
                     /locus_tag="VGC82_17165"
     CDS             1420..>1629
                     /locus_tag="VGC82_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425053.1"
                     /translation="MADAPGNDRIRLTADEARALGEAAMRGAGFDNEDARILTDHVLD
                     AALCGYEYSGLPKLLNVVDYPNFRQP"
BASE COUNT          301 a          557 c          485 g          286 t
ORIGIN      
        1 aatggtttga cttgacgcca acgacccgtg tctcattcgg ctgtcagttg aatgggacac
       61 ggtatttctg actgtgccgt ggtgggagaa ggcaagacat cctgacaccg tcaacagcgg
      121 gggttcgggt tggtgagacc cgacccgctt cacccaaacg ccctcctgat ccgtccccaa
      181 tcctcgatcg cgtgttcctg acccggcggg atcgagaata cgacctgatc gaaccccgcg
      241 tccgccagcg cgccaattcg ttgcgtgaga tccgcggccg tgccgatgaa actggtgcgc
      301 cggatcatct cggcggtgat gaaccgccgt tcgtccggcc ggacgaacat cagatgcccc
      361 cgatggttcg acaggtaata ggcatccttt ggttcgaacc ctcgggcatg ctccacatac
      421 gcctccacgg cgtcagcgaa ctccggccgc atcgccaccg gtgacgggta acccgcaagc
      481 gccgtatcgg ccgctctgtg cagcagcatc gccgctcggg gtgccgccag cgccataccg
      541 cgcgggccat ccaccggctc cccgtcttcc agtaccgcgc cgccgatcca cgcgaccgag
      601 gtcaacgcct cgcgcttatg ccccgcatcc tgccaggcga tccgcatctg ctccaacgtt
      661 gccgcgccgc gctccgcgtc cgccacattg tcgatccatc ccgccgcgag tttggcggtc
      721 agcgcccggg ccttcggacc tgaagcagcg atataggtcg gcatcggatc atcggtgttg
      781 ataagcccga gttccgggtt cagcagccgg atcttgcgca gccggccttc caccgcgaat
      841 tccaccgtct caccgcgcca cagcgcctgg atgacgcgga tgtactgctc catatccgcc
      901 agcttcaccg ccccaaggcc catcgtccgc cgcccggtaa acccggtgct gacgccgaaa
      961 atcactcggc ccggcgcaag cttgttcagc gaggcaaaag cggtggccgc gactggcgcg
     1021 atccggttgg acgggatcaa cacgccggtg ccgagtttga tccgcgaggt cttcattgcc
     1081 gaagccgcca tggcgacgaa cggatcggca ctcaacatct gtgtgtcgaa gaaccaggca
     1141 tggctgaaac ccagttcctc cgcgcgcctc accaggcgcc aggagtccgc cgccgtggga
     1201 atagcgattc cgaactgcat cgatcgttcc tccctgattg gccggatgca acgccgataa
     1261 gcgcgtccgt tcgcgcgact ttaaggctgc gcccaacaat gaccaaatct gctgacgtga
     1321 ctcggcgata cagtttcggg ggtttcggcg cggcgagtga gatggacacg gattgcgatc
     1381 cggaggcacg ctggtgcagc caacaacaag gatcgaacaa tggccgacgc gcccggcaac
     1441 gaccgcatcc gcctgacagc cgatgaagcc cgcgcactgg gggaagccgc gatgcgcggt
     1501 gccgggttcg acaacgaaga cgcgcggatc ctgacggatc acgtcctgga tgcggctttg
     1561 tgcggctatg agtattccgg cctgccgaaa ctgctgaacg tcgtcgacta cccgaatttc
     1621 cggcagccc
//
LOCUS       DASWQN010001008         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1944387, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001008 DASWQN010000000
VERSION     DASWQN010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944387"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(596..1315)
                     /locus_tag="VGC82_17170"
     CDS             complement(596..1315)
                     /locus_tag="VGC82_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425054.1"
                     /translation="MLTISNLQVRYGDLVGVADVSLVVPAGSVVALLGSNGAGKTTTL
                     NAIAGLVPPAGGTVTFQGQAITGKPAFAIVAAGLALAPEGWRLFVQQTVEQNLRLGAT
                     VLKDKTRMPALLDRVFTLFPRLAERRRQRAGTLSGGERQMLATGRALMSDPKLLMLDE
                     PSLGLAPAVVELMYETLARLRREGLTLLLAEQAVELALEVADYAYVLQTGRTVLEGPS
                     GELAGDKDVQRIYLGLETEGR"
     gene            complement(1359..>1991)
                     /locus_tag="VGC82_17175"
     CDS             complement(1359..>1991)
                     /locus_tag="VGC82_17175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425055.1"
                     /translation="LIGPNGAGKSTFFNCLSGDAEPTQGQIRFLDQDVTQSSPEAHAR
                     LGMARTFQVPVTFEDMTVLENVMVGAFLRHPRRSRAVQKAAEVLEFTGLSHLSGSPAR
                     SLGTPGRKRLEIARALATEPKLLLLDEALAGLTPAEVRTAIELVRRIQGLGITIVIVE
                     HIMEVILSLAERAVVFHQGKVIAQGAPRAVVDDPAVVTAYLGHHMKRVRM"
BASE COUNT          362 a          685 c          589 g          355 t
ORIGIN      
        1 gaagcgagtt cttgccgtgg ccagacgcgg gtattgggct gaatgaagta ttgatggagg
       61 aagctggcca caagcggcgc atggtgggcg gccagcattc tccatgccgg atgggtccgt
      121 cgcaaactgt ccagggtccg gtagtccatg ccatcactct atcggcggtt atcgtcgaac
      181 ttcggttcga cggcccgggc gcatcgatcg gtagggttcg agttttgttc tgtggtgtca
      241 agggggattg ctccggtttg catccgttcg gactagggta gttcggggct ggatctttca
      301 tcacgggttg ctggattcaa cccggatttg taaggaaagc caggtggcgg cagtctttgc
      361 gacttttggc gtcggtttgg tgccaggtcc accgcatggc gctgcttgcc ggcgtgacag
      421 tgcggcaccc agaccccgaa cgagaccctc cgcgcgaagc gcccacatac cgcgagcaaa
      481 ccacggatag cgtgtccggc cgcaccaaat caaaacgact tccttgccag tccgcgccgt
      541 aatctgtgat gaacgctttc ttccacccga cgtcccgccc cacaacaagc ccccgtcaac
      601 gcccctcagt ctcaagcccc agataaatcc gctgcacgtc cttatccccg gccagttcgc
      661 ccgaaggtcc ctccagcacc gttcgcccgg tttgcaggac ataagcgtaa tccgccacct
      721 ccaaagccaa ctccacggct tgttcggcca gcagcaaggt cagtccttcc cgtcgcaacc
      781 gcgccagcgt ctcgtacatc aactcgacca cggccggcgc cagccccagg ctgggttcat
      841 ccagcatcag cagcttcggg tcgctcatga gtgcgcggcc agtcgccagc atctgccgct
      901 caccgcctga cagcgtcccc gcccgctgcc gccggcgctc tgccaaccgc ggaaacaagg
      961 tgaagacgcg gtccagcaac gccggcatac gcgtcttgtc cttcaacacc gtggcgccga
     1021 ggcgcagatt ctgctccacc gtttgctgca cgaacagccg ccagccttcc ggcgccaatg
     1081 cgaggccggc ggcgacgatc gcgaaagcgg gtttgccggt gattgcctgt ccttggaagg
     1141 tcacggttcc gcccgccggt ggcaccagcc cggcaatggc attcagcgtc gtcgtttttc
     1201 cggcaccgtt ggaacccagc agcgccacaa ccgaacccgc cggcacgacc agcgaaacat
     1261 cggccacgcc caccagatcg ccatagcgaa cctgcaggtt ggagatggtc aggatcggag
     1321 tggcggtcat gcgcgccgga cccgatcggg agtggccatc acatccgcac ccgcttcatg
     1381 tggtgcccaa gatacgccgt caccaccgcc ggatcgtcaa caaccgccct tggagcaccc
     1441 tgcgcgatga ccttcccctg atgaaacacg accgcgcgtt cggccagcga caggatcacc
     1501 tccatgatgt gctccacgat cacgatggtg atccccaacc cctggatgcg acgcacgagc
     1561 tcgatcgccg tgcgcacctc ggccggcgtc aacccggcga gagcctcgtc cagcagcagc
     1621 aatttcggct ccgtcgccaa cgcccgggcg atttccaggc gcttgcgccc cggcgtcccc
     1681 agcgagcggg cgggtgaacc ggacagatgc gacaaccccg tgaactccag cacctccgcc
     1741 gccttttgca ctgcccggga tcggcgggga tgacgcagga aggcgccgac catgacattc
     1801 tcgagcacgg tcatatcctc gaacgtcacc ggcacctgga atgtccgcgc catgcccagg
     1861 cgcgcatggg cttccggact ggattgggtc acgtcctggt ccaggaagcg aatttgtccc
     1921 tgtgtcggtt cggcgtcgcc ggacaggcag ttgaagaagg tgcttttgcc ggcgccgttc
     1981 gggccgatca g
//
LOCUS       DASWQN010001009         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1020653, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001009 DASWQN010000000
VERSION     DASWQN010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020653"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1462
                     /gene="ahcY"
                     /locus_tag="VGC82_17180"
     CDS             158..1462
                     /gene="ahcY"
                     /locus_tag="VGC82_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY0425056.1"
                     /translation="MATAATQDFKVKDLSLADWGRKEISMAETEMPGLMAIREEFGNS
                     QPLKGARIAGCLHMTIQTAVLIETLTALGATVRWSSCNIFSTQDHAAAGIAATGVPVF
                     AWKGLSEEEFWWCIEQTVRGPDGWTPNMILDDGGDLTVLMHQKYPQMLADVRGISEET
                     TTGVHRLWDMAKAGTLLTPAINVNDSVTKSKFDNLYGCRESLVDGIRRGTDVMMAGKV
                     AVVNGFGDVGKGSSASLRNAGCRVMVTEVDPICALQAAMEGYQVVTMDDAAPVGDIFV
                     TATGNVDVITLEHMRAMKHRAIVCNIGHFDSEIQIESLRNYKWENVKPQVDEVVFPDG
                     KRLIVLSEGRLVNLGNATGHPSFVMSASFSNQVLAQIELYNAPPGKYERTVYTLPKHL
                     DEKVAALHLAKVGAKLTTLSPKQAEYLGIAEQGPFKHDLYRY"
BASE COUNT          360 a          489 c          516 g          316 t
ORIGIN      
        1 gaccattcgt cccccgaccg ccgcagcgac caaaagggga cattctagct ttggacgatg
       61 gggacattcc tacttggggc taacaaccct cactgcggtc ttgcaaacat gcagatatct
      121 ttatatatcg ccgcgcatac ccggagcttt cagaatcatg gccactgcag cgacacaaga
      181 tttcaaggta aaagaccttt ccctcgctga ttggggccgc aaggaaatca gcatggccga
      241 gacggaaatg cccggcctga tggcgatccg cgaggaattc ggcaactccc agcctttgaa
      301 gggcgcccgc atcgccggat gcctgcacat gaccattcag accgccgtgc tgatcgagac
      361 gctgaccgcc ctcggcgcga cggtgcggtg gtcctcgtgc aacattttct cgacccagga
      421 ccatgccgcc gccggcatcg ccgcgaccgg ggtacccgtt ttcgcctgga agggcctgag
      481 cgaggaagaa ttctggtggt gcatcgagca gacggttcgt gggccggacg gctggacgcc
      541 gaacatgatc ctcgacgacg gtggcgacct gacggtgctg atgcaccaga aatatccgca
      601 gatgctggcc gatgtgcgcg gaatctccga agaaaccacc acgggcgtgc atcggctgtg
      661 ggacatggcg aaggcaggga cgctgctgac gccggcgatc aacgtcaacg acagcgtaac
      721 gaaatcgaag ttcgacaacc tgtatggttg ccgtgagtcg ctggtggatg gcatccgccg
      781 cggcaccgac gtcatgatgg ccggcaaggt cgccgtggtg aacggctttg gcgatgtcgg
      841 caagggttcg tcggcttcgc tgcgcaacgc cggttgccgc gtaatggtga ccgaggtcga
      901 tcccatctgc gccttgcagg ccgcgatgga gggctatcag gtcgtaacga tggacgacgc
      961 ggcgccggtg ggcgacattt tcgtcaccgc cacaggcaat gtggacgtga tcacgctgga
     1021 acacatgcgc gcgatgaagc acagggcgat cgtgtgcaac atcggccact tcgactctga
     1081 aatccagatc gagtcgctgc gcaactacaa gtgggaaaac gtcaagccgc aggtggacga
     1141 ggtggtcttc ccggacggca agcgcctgat cgttctgtcc gagggccgcc tggtcaacct
     1201 gggcaacgcg acaggccatc cgagcttcgt catgagcgcc agcttcagca atcaggtgct
     1261 ggcacagatc gagctttata atgcgcctcc tgggaagtac gagcgtacgg tttacactct
     1321 gccgaagcat ctcgacgaga aagtcgccgc gctgcatctg gcgaaggtcg gcgcaaaact
     1381 cacaaccttg tcaccaaagc aggcggagta tctcgggatc gccgaacaag ggccgttcaa
     1441 gcacgacctg taccggtatt gatggcatcg ggaatccaaa ttcaccagct tgtcatgctc
     1501 gtgtgctagt gactcgccgc aacaaagacc taactcaaga ccgtgctgtt cggtggattt
     1561 gccctcggct gttacccggg aagaatttgc cgaacagcgt tgagactgat taggaaaatg
     1621 acatgttgtc ggcctcttcc gaactgacgc cgccgttcac gcttgcggtg cggcaggcgt
     1681 t
//
LOCUS       DASWQN010001010         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_625174, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001010 DASWQN010000000
VERSION     DASWQN010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625174"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..518
                     /locus_tag="VGC82_17185"
     CDS             90..518
                     /locus_tag="VGC82_17185"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY0425057.1"
                     /translation="MSCLTSTSVGNPGCGCCPLSSRSCRIRPGLVRRSSRNRSALRVS
                     GIALRPFHRLPAQRRTREVLDLLAQGLSDTAIAAKSDVTSDTVRNHVAAIYKELGVNR
                     RGTAVAWAREHGFGAKPKILTKLINPGAANGRSKFVQGTR"
     gene            777..1187
                     /locus_tag="VGC82_17190"
     CDS             777..1187
                     /locus_tag="VGC82_17190"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEY0425058.1"
                     /translation="MSEPAKHEDGNTVVLPLHGETLAVSRRKVETSVVQVSTVTQSND
                     ALVDEALSHERVEIERVPVGRIVATAPSVREEGDVTIIPVLEETIVIEKRIVLKEEIR
                     VRRVRVTEQHRETVVLREQEVVIKRSETGRAPES"
     gene            1266..2264
                     /locus_tag="VGC82_17195"
     CDS             1266..2264
                     /locus_tag="VGC82_17195"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEY0425059.1"
                     /translation="MTDETIVAVYDSAAHADAAVRDLESINVPREAITRHSGSSTTGN
                     PGSAEPARERGFWSSLFGGDPGPDQEVYDRSMASGSSVVMVKVPDQHVQRVTDVLERH
                     NPIDIDERAAGYGIGMGGTSATTTTTAGMPATGATMRGATMGDATTGDATMGTPAPGL
                     AGSGGMASGTAGSGMSTTGASSSTMPHGGEEVLPLSEEELTIGKRLINRGTTRIRRFV
                     VETPVEEAVNLHSEHVSVERRPVSGSANVANPEFTDRTVEFTETDEEAVVAKTAHVKE
                     EVVINKEATDRTETVRDTVRREDVEITKDASTTPGTSTSGTDKISDRSVDPTRPRI"
     gene            2319..3245
                     /locus_tag="VGC82_17200"
     CDS             2319..3245
                     /locus_tag="VGC82_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425060.1"
                     /translation="MSANTMNDPAYRGSTTIETTTRLDGSHIHRRISWAAIFGGVILV
                     LAVQLLLGMLGAGVGLGTVNVNGGSTPDASTFGMGAALWWIISSVIALGFGGYVSAWM
                     AGIEIRWDGVLHGLVTWGIATLLTIYLLTSAVGGVIGGGFSALGSAASAAGNGVKDAA
                     QPVAQAAGVSPDMVQQQAQAYLQPPNPDPATLSPQDAQKQVATNLATYAKGGSDAPAA
                     KERIINIMAAQQHISHDDAAKQFQDTQAKLQQTKDQAAQTAKNAADATASAASKTSFA
                     VFVNLLIGAIVAAIGGSLAVQRRLNVTQGVVR"
BASE COUNT          664 a         1002 c         1006 g          589 t
ORIGIN      
        1 ccggacacat cagcagcatc ggcactggat ttgtgaccac ctgctgtcgg cggaaaccgt
       61 cacggtccag caggagatgt gcatcttaaa tgtcatgctt gacatcaacg agcgtcggca
      121 acccgggatg cggttgctgt ccgctatcga gtcggtcatg caggatacgt cctggcttgg
      181 tcagaagatc gtcgagaaac cgatcggcat tacgggtgtc ggggatcgcc ctcaggccgt
      241 tccacagatt gccagcccaa cgccgcaccc gggaggtgct cgacctgctg gcgcagggtc
      301 tttccgacac cgccatcgcc gcgaagtcgg atgttacatc cgataccgtt cgtaatcacg
      361 tcgccgcgat ctacaaagaa cttggcgtca accgccgcgg cacggccgtc gcctgggcgc
      421 gcgagcatgg ttttggagca aagccgaaga tattgacaaa gctgataaat cccggcgccg
      481 caaacggccg cagtaaattc gtccagggta cccgataatt ttcgtcttgg cctgctcggt
      541 tttgtcttcg cggcgttcgg cttcccgtgg tggaacttat cgccacgcac cgcgctgtgc
      601 ctgtgcgaag cagcaagcgt ccgactgggg caattcgtgg tcctgattcg tcgccggcgc
      661 tgtgtgacat gctgtcgggc atagggaaac cgccgcagaa gcggacggtt tatactgccc
      721 ttactgtccc ttcgaagatc aagagttaca agtggataag gatcgcgaca ccgccaatga
      781 gtgaacccgc gaaacacgaa gacggcaaca cggttgtctt gccactacac ggcgagacgc
      841 ttgccgtgtc gcgccggaag gtcgaaacat cggtcgttca agtctcgacg gtgacgcaat
      901 cgaacgacgc gctggtcgat gaggcgctta gccatgaacg ggtcgagata gagcgagtgc
      961 cagtcggtcg gattgtggca accgctcctt cggtccgcga agagggcgac gtcacgatca
     1021 ttccggttct cgaggaaacc attgttatcg agaaacggat cgtcctcaaa gaggaaattc
     1081 gcgttcgacg ggtgcgcgtc acggaacaac accgggagac cgtcgttctg cgggagcagg
     1141 aggtcgtcat caagcgcagc gagaccggcc gggcgcctga gagctgaacc acaccggccc
     1201 ccatgatgca gtcgccttcg ctagccgcag ttgtctttca ctaaccacac aagaggttga
     1261 acaccatgac cgatgaaacc atcgttgctg tttacgatag cgccgcacac gccgatgccg
     1321 cggtacggga tcttgagtcg atcaatgtgc cccgcgaggc gatcacccgc cattccggca
     1381 gctcgacgac gggcaacccg ggctccgcgg aacccgcccg tgaaaggggc ttttggtcca
     1441 gcctgttcgg cggcgatcca ggaccggacc aggaggtcta tgaccgcagc atggcgagcg
     1501 gttcgtccgt ggtgatggtg aaggttccgg accaacatgt gcagcgggtg accgatgtgc
     1561 tggaacgcca caacccgatc gacatagacg agcgcgccgc cggatatggc atcggcatgg
     1621 gcggcacctc cgccaccacg acgacgacag ccggaatgcc ggcgacgggc gcaacgatgc
     1681 gcggcgcgac catgggcgac gcaacgacgg gcgacgcaac catgggcacc ccagcccccg
     1741 gactggccgg ctcaggcggg atggcttcgg gtacggcggg gtcgggtatg tccaccaccg
     1801 gggcatcctc gtccaccatg ccgcacggcg gcgaagaggt tcttccgttg tccgaagaag
     1861 aactgaccat cggcaagcgc ctgatcaatc gcggcaccac ccgcattcgc cggttcgttg
     1921 tcgaaacgcc ggtcgaagag gccgtcaatc ttcattccga acatgtctcg gtcgaacgcc
     1981 gtccggtatc cggtagcgcc aacgtcgcca accccgagtt caccgatcgc acggtagagt
     2041 tcactgaaac cgatgaggaa gccgtcgtcg ccaagacagc ccatgtcaag gaagaggtcg
     2101 tgatcaacaa ggaggccacc gaccggaccg agacagtgcg cgatacggtt cgtcgcgaag
     2161 atgtcgagat caccaaggac gcaagcacga ccccagggac atcgacatcc ggcacggaca
     2221 agatctccga tcgcagcgtc gatccgactc gtccgcgcat ctaactcgtt acagtgcggg
     2281 ccgggacgcc attgttccgg ccggcctggg aggaacctat gtctgcaaat accatgaacg
     2341 atcctgcgta ccggggatcg acgacgatcg agacgaccac ccgcctcgat ggttcccaca
     2401 ttcatcgtcg catatcctgg gccgcgattt tcggtggcgt catcttggtt ctggccgtcc
     2461 aattgctgct tggcatgctg ggcgccgggg tcggacttgg cactgtcaac gtcaatggcg
     2521 gctccacccc cgacgccagc accttcggca tgggcgctgc gttgtggtgg attatcagca
     2581 gcgtcatcgc gcttggcttc gggggctatg tgtcagcctg gatggctggg atcgagatca
     2641 ggtgggacgg cgtcctgcac ggtcttgtga cctgggggat cgccacgttg ctgacgatct
     2701 acctgttgac ttcagcggtc ggcggcgtca tcggcggcgg gttctccgcc cttggcagtg
     2761 cggcgtcggc ggccggtaac ggcgtgaagg acgcggcaca accagtagcc caggcagccg
     2821 gcgtgtcgcc tgacatggtc cagcagcagg ctcaggccta tcttcagccg ccgaaccccg
     2881 acccggcgac cctgagtcct caggatgcgc aaaagcaggt cgcaaccaac ctggcgacct
     2941 atgccaaagg cggttccgat gccccggcgg caaaggagcg catcatcaac attatggcag
     3001 cacagcagca tatcagtcac gatgatgcgg ccaagcagtt ccaggatacc caggcgaagt
     3061 tgcaacagac gaaggatcag gcggcgcaaa cagccaaaaa tgctgccgat gcgactgctt
     3121 ccgccgcttc caagacctct ttcgcggtgt tcgtcaacct gcttattggc gcgatcgttg
     3181 ctgccatcgg tggttcgctc gccgtccagc gccgcctgaa cgtcacccag ggcgttgttc
     3241 gctaatatct ggcactgata t
//
LOCUS       DASWQN010001011         3042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2340108, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001011 DASWQN010000000
VERSION     DASWQN010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340108"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VGC82_17205"
     CDS             complement(<1..453)
                     /locus_tag="VGC82_17205"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY0425061.1"
                     /translation="MITGRTRLIVHLGFPTESFKAPMIYNPWFEMKGIEAIVVPMGVE
                     PPNYPDLLKALFRTSNVHGALVTMPHKVTTTHLVDEMTTTAKIAGACNAILRRPDGSL
                     LGDMFDGAGFTRGVERKGKRIAGARALVVGCGGVGSAIAASLAAGGLSA"
     gene            complement(546..995)
                     /gene="aroQ"
                     /locus_tag="VGC82_17210"
     CDS             complement(546..995)
                     /gene="aroQ"
                     /locus_tag="VGC82_17210"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY0425062.1"
                     /translation="MSRIVFVLNGPNLNLLGKRQPHIYGHETLADVERDCAALAKELD
                     LELRFHQSNREYEIIDWIHEARQIAGGIVINPAAFTHTSVAILDALNTFDAPVIEIHI
                     SNVHKREAFRHHSFVSSRANGVIAGFGTQGYLLALRRVARLIDEKGR"
     gene            complement(1090..2499)
                     /locus_tag="VGC82_17215"
     CDS             complement(1090..2499)
                     /locus_tag="VGC82_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425063.1"
                     /translation="MDSATDQEPHRARQTKKATASGWIGSALEYYDFFIYATAASLIF
                     PQIFFPSGNPTVAIVASLATYGVGYVARPIGAFFLGHWGDTHGRKTVLLVCMFLMGFS
                     TMAVGLLPTYQQVGMLAPTLLVILRLIQGFAVAGEISGASSMILEHAPFGRRGFFASF
                     SLQGVQAGQIFAAAIFLPLAHYLPTEAFNTWGWRIPFLLSVIVIIAGYIIRREVEETP
                     AFAEADEHGVVPRAPIIQAFAQSWRDMLRVVCMALMNVIPVVTAIFGAAYAVQPGYGI
                     GFQKDIFLWIPVLGNILAVAVIPVVGNLSDRIGRRPPIIVGAIVCGLLSFGYLYAISI
                     ANVPLAIAMSLLMWGVVYQGYNSVFPSFYPELFPTRTRVTAMAISQNVGTTITALLPA
                     LFASVAPPGSTNVPLTIGAITFGVTLVAAVAAWSARETFRIHLNDLGEPNAVPVDKAT
                     YDRMRIRATSGADVAGASA"
     gene            complement(2645..>3042)
                     /locus_tag="VGC82_17220"
     CDS             complement(2645..>3042)
                     /locus_tag="VGC82_17220"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425064.1"
                     /translation="MITPKIGHRGIGIAGFAIVLVSLLVAAGALYTGKTYILPFAAAA
                     MLWGHYWDASNCMTIPTVVAKPEYRGTASGFAYMFVKLPSFLAIFLFPALFSAIGQAN
                     ATLFVAIFPLIGLLAAIFILPEIYGYEHD"
BASE COUNT          615 a          927 c          971 g          529 t
ORIGIN      
        1 ggccgaaagc ccgccggcgg ccagcgacgc cgcgatggca gagccgacac cgccgcagcc
       61 gaccaccagc gctcgagcgc cggcgatgcg cttgcccttg cgttccacgc cgcgggtgaa
      121 gccggcgcca tcgaacatgt cgccgagcag cgacccgtcg ggccggcgga ggatcgcgtt
      181 gcacgcgccc gcgatcttcg cggtcgtggt catctcgtcc acgagatgtg tcgtcgtcac
      241 cttgtgcggc atggtgacca gggcaccgtg cacattcgac gtgcgaaaca acgccttgag
      301 cagatcgggg tagtttggcg gctcgactcc catcggcacc acgatcgcct cgatgccctt
      361 catctcgaac caggggttat agatcatggg cgctttgaac gactcggtcg gaaagccgag
      421 atggacaatc agcctggtcc tgccggtgat catggttgcg ctccggaagc cgctaatcgg
      481 ttgtggatat ggtactcgat gccggcgggt tcgcctgccg gccgccatcc gatcgggaag
      541 ttgacctacc ggcctttttc gtcgatcagc cgtgcgacgc ggcgcaaagc cagcaggtac
      601 ccctgcgttc cgaagcccgc gatgacgcca ttggcgcgac ttgagacaaa cgaatggtga
      661 cggaaggcct cccgcttgtg gacattggag atgtggatct cgatcaccgg cgcatcgaat
      721 gtgttcaagg cgtcgaggat cgcaaccgaa gtatgggtga atgcggccgg gttgatcacg
      781 attccgcccg cgatttgccg cgcctcatgg atccagtcga tgatctcgta ttcgcggttg
      841 ctttggtgga agcgcagctc gaggtctagt tccttggcca gcgccgcgca atcgcgttcg
      901 acgtcggcca aggtttcatg gccgtagatg tgcggttggc gcttgccaag caggttgagg
      961 ttcgggccgt tcagcacgaa aacgatgcgg ctcatcacag cctccttgat gcggtcgccg
     1021 ccgccggtgt ctcattctgg gccattattc acatggaccg agcgttcaaa gcccgccggg
     1081 cgccgccgtt catgcggagg ctcctgcgac atccgcacca gaggtagccc gtatccgcat
     1141 ccggtcgtag gtcgctttgt caaccgggac ggcattgggt tcgcccaggt cgttcaggtg
     1201 tatccgaaag gtctctctcg cgctccaggc cgcaaccgcc gccacgagcg tgacgccaaa
     1261 tgtgatcgcg ccaatggtaa gcgggacgtt ggtcgatccg ggcggtgcga cggacgcgaa
     1321 cagggcggga agcagggcgg tgatggtcgt tccgacgttc tgcgatatcg ccatcgccgt
     1381 cacacgcgtg cgcgttggga acaattccgg atagaagctt gggaagacgg agttgtagcc
     1441 ctggtaaacg accccccaca tcaacagcga catggcgatc gccagcggca cgttggcgat
     1501 actgatggca taaaggtagc cgaacgacag caacccgcac acaatcgcgc cgacgatgat
     1561 agggggcctg cggccaatcc tgtccgacag gttgccgacg accgggatga cggccacggc
     1621 caggatgttc cccagcaccg ggatccatag gaaaatgtcc ttttggaagc cgatcccata
     1681 cccgggctgt accgcgtagg ccgcgccgaa tatcgcggtt acgaccggga tgacgttcat
     1741 caacgccatg caaacgaccc gcagcatgtc cctccagctt tgcgcgaagg cctggatgat
     1801 cggtgcccga gggaccacgc catgctcatc cgcttcggca aaggccgggg tttcttcgac
     1861 ctcacggcga atgatgtagc ccgcgatgat gacgatgacg ctgagcagga agggaatccg
     1921 ccagccccag gtgttgaacg cctcggtcgg caggtagtgc gcgagcggca ggaagattgc
     1981 ggcggcgaag atctgccccg cctgcacgcc ttgcaggctg aaactggcaa agaagccgcg
     2041 ccgaccgaat ggggcgtgct cgagaatcat cgaactggca ccggagattt caccggcgac
     2101 cgcgaacccc tggatgaggc gcaggatgac gagaagcgtc ggcgcgagca tgccgacttg
     2161 ctggtaagtg ggcagcagac caaccgccat ggtcgaaaaa cccatcagaa acatgcagac
     2221 gagcagcacc gtcttgcggc cgtgagtatc gccccagtgt ccgaggaaga acgctccaat
     2281 gggccgggcg acatagccga caccataggt tgccaatgac gcgacgatgg cgaccgtcgg
     2341 gttcccggac ggaaagaaga tctgcgggaa aatgagcgac gccgcggtgg cgtagataaa
     2401 gaaatcatag tattccagtg ccgaaccaat ccagccgctg gctgtcgcct tcttggtctg
     2461 acgggcgcgg tggggttcct ggtccgtggc gctatccatt gtgagtccct ccgatgcctt
     2521 gtcgtcgttg ctggataatc agaccaccga gccataagat cgaccaatcc gaccccgacc
     2581 cgctgggact gtcaacttcc tccggggcgt ctgctgacaa accgcacgct gccgctgcaa
     2641 taccctaatc gtgctcgtag ccatagatct ccggcaggat aaagatggcg gcgagcaacc
     2701 caattagcgg gaagatggca acgaacagcg tcgcgttagc ctggccgatc gccgagaaca
     2761 acgccgggaa caggaagatc gcgaggaacg acggcagctt gacgaacatg taggcgaagc
     2821 cgctcgcggt accgcgatac tcgggcttgg cgaccaccgt cgggatcgtc atgcagttcg
     2881 aagcgtccca atagtgtccc cacagcatgg cggcggcggc gaacggcaga atataggtct
     2941 tgccggtgta aagcgccccc gcggcgacca gcagcgacac cagcacgatg gcgaaccctg
     3001 cgatgccgat tccgcggtgc ccgatcttcg gcgtgatcat gg
//
LOCUS       DASWQN010001012         2936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2077232, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001012 DASWQN010000000
VERSION     DASWQN010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077232"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="VGC82_17225"
     CDS             <1..1105
                     /locus_tag="VGC82_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0425065.1"
                     /translation="LNAAGAYSFAGLNMAEFAQNPTGHNHAFGDCHNPWNLPYITGGS
                     SSGSGASVAARFNYMALGSDTGGSIRLPASACGVTGLKPTQTRVSRYGVMPLSFSHDN
                     VGPLARTARDCARVLSVIAGHDPLDPTSALEPVPDYERSMNGDLRGLRIGVPTAFFLD
                     GADDPVLAAMETALRVLAERGATINRFDLPLMDAVAAYGGIVSRVEAAPIHAEWMRSD
                     PQAYGVHISGRMYPGYAIPAAYYVEALSRRGPVLKAFAADVFAKVDVLVTPTIRTCLP
                     TLAETDIDHGPPGTEHRFMAVSANTRPFNYLGLPAVSIPCGFDPNGCPIGLQIAGRPF
                     AEARILNVADAYQSDTDWHMKRPAILESAGVAA"
     gene            1102..2100
                     /locus_tag="VGC82_17230"
     CDS             1102..2100
                     /locus_tag="VGC82_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY0425066.1"
                     /translation="MSKPKVAFIGTGGTIASLGRGPLDLQDYGAAGNVMHADEILGRW
                     PEASLVADVMAVRYRNIASPAIGFAEWKDLATLCRQLVSDHADLAGIVIGHGTATLEE
                     TAYFLNLTVKVAVPVVLVGSQRPSSALSSDAGLNLVNAIRVAACPEARDMGVLVVLND
                     EIHAAREVTKTSTARLQTFRTPDFGVLGHADGDAVVFYRRPVRRHAPNTEFSVAELDT
                     LPRVDISYSYAGGDGVAVRAFMAAGARGIIAAGFAPGYCPPGEVEALKEAARQGIVVV
                     QSTRAGSGRTFRGTRLRQAGFLIADNLNPQKARILLSLALTVTTDPETIMRMFRTY"
     gene            complement(2338..>2936)
                     /locus_tag="VGC82_17235"
     CDS             complement(2338..>2936)
                     /locus_tag="VGC82_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425067.1"
                     /translation="ILPAIAREGGGTPWISGQVTSFHALRIKPPFYFEARAKMPVGIG
                     RPWPAIWLLTGAHRHPGDSGKEYEIDLHEGFGDDDRLHSAIHWNPRADKPEYPSSEVA
                     NMPSGVILSGGFNTWACKVTKGLQIVFFNGKEIGRLETPKTANADQFYGMVLDVSAGL
                     PWVNGLRNDGGAPSGGPHDMIVRYVRLYAPDTNGLRLR"
BASE COUNT          543 a          944 c          897 g          552 t
ORIGIN      
        1 cctgaacgcc gccggcgcct acagcttcgc cggcctgaac atggccgagt tcgcgcagaa
       61 ccccaccggc cacaaccatg ccttcggcga ctgccacaac ccctggaacc tgccgtatat
      121 caccggcggg tcctcgtccg gatcgggagc ctccgtcgcc gcgcgattca actacatggc
      181 gcttggttcg gacaccggcg gatcgatccg gctgccggcc tcggcctgcg gcgtgaccgg
      241 gctgaaaccg acccagacgc gggtgtcccg ctatggcgtg atgccgctgt cgttttcgca
      301 cgataatgtc ggtccactgg cccgaaccgc gcgcgactgc gcaagggttc tcagcgtcat
      361 cgccggccac gatccgctcg accccacaag cgctctggaa ccggtgccgg actacgagcg
      421 gtccatgaac ggcgacctgc gtggcctgcg catcggagta ccgacggctt tcttccttga
      481 tggcgcagac gacccggtcc tggcggcgat ggagacggcc cttcgcgtcc tggccgaacg
      541 aggtgcgacc atcaaccgtt tcgacctgcc gttgatggat gcggtggcgg cctatggcgg
      601 cattgtctcc cgcgtcgagg cggcgcccat ccatgcggaa tggatgcgct ccgacccgca
      661 ggcctatggc gtgcatatca gcgggcggat gtatcccggc tatgccattc cggctgccta
      721 ctacgtggaa gcgctcagcc gccgcgggcc ggtgctgaaa gccttcgctg ccgacgtatt
      781 cgccaaggtc gatgtcctgg taacgccgac catccgaacc tgcttgccga cgctggcgga
      841 aaccgacatc gatcatggac cgcctggaac cgagcacagg ttcatggcgg tgtcggcgaa
      901 tacgcgcccg ttcaactatc tcggtttgcc cgcggtcagc atcccctgcg gcttcgatcc
      961 caacggctgt ccgatcggct tgcagatcgc cggccgcccc ttcgccgagg cccgtattct
     1021 gaacgtcgcg gatgcctacc agtcggatac cgactggcat atgaagcgac ccgcgatcct
     1081 tgaaagcgcc ggagtggccg catgagcaag ccgaaggtcg cgttcatcgg gacaggcggg
     1141 acgatagcgt cgctgggacg cgggccgctg gacctgcagg actacggggc ggcaggcaat
     1201 gtgatgcacg ccgatgaaat cctggggcga tggccggaag catcgctagt cgccgatgtc
     1261 atggccgtgc gttaccgaaa catcgccagc ccggcaatcg gtttcgcgga atggaaggac
     1321 ctggcgacgc tgtgccgcca gctcgtcagc gatcacgccg atctggccgg catcgtcatc
     1381 ggccatggca cagcaacgct ggaggaaacc gcctatttcc tgaacctgac cgtcaaggtc
     1441 gcggttccgg tggtgcttgt gggatcgcag cggccatcga gcgctttgtc cagcgacgcc
     1501 ggcctcaatt tggtcaacgc catccgcgtc gctgcctgcc ccgaggcccg ggacatgggt
     1561 gtgctggtgg tgctgaacga cgaaatccac gccgcacgag aggtgaccaa gacctcgact
     1621 gcgcgcttgc agacatttcg gacgccggat ttcggcgtgc tgggtcatgc tgacggcgat
     1681 gcggtggtct tctaccggcg gccggtccgt cggcatgcac cgaacaccga gttcagtgtc
     1741 gcagaactcg acacgctgcc tcgggtcgat atttcctact cctatgccgg cggcgacggc
     1801 gtggcggtgc gagccttcat ggcggccggc gccaggggga tcatcgcggc gggcttcgca
     1861 ccgggctatt gcccgcccgg agaagtcgag gcgctgaaag aggccgcacg gcaggggatc
     1921 gtggtcgtac agtctacccg ggccgggtcc ggccggacgt ttcggggtac caggttgcgg
     1981 caggccggct tcctgatcgc cgacaatctg aacccgcaga aggctcggat tctactgtcg
     2041 ctggcactga ctgtcaccac cgatccggag acgatcatgc gtatgttccg gacgtattaa
     2101 ttttgtttcg taacaatatg tgcaatcgcg catctgacga tccgaacaat cgcacgccag
     2161 catgccgctg aaaataggca cgccactgca agccgcccga tcccgctaca agcttcaggc
     2221 atatcgcacc tggcagaagc actgcccgtg gtggctacct gttgctgtcg tggggagctt
     2281 tacaggatga gaccaacaag caacgcgaac gccgtcatcg tctttgataa ggggcggcta
     2341 tcgaagcctg agtccgttcg tgtcgggtgc atagagccgc acgtagcgaa caatcatatc
     2401 atgtgggcca ccgcttggtg cgccaccatc gttccgcagg ccattcaccc acggcaaacc
     2461 cgcactgacg tctaaaacca taccataaaa ctgatcggca ttcgcggttt ttggtgtttc
     2521 aagccgacca atttcctttc cgttgaaaaa gacaatctgc agtcccttgg ttactttgca
     2581 agcccaggta ttgaatccgc ccgacaggat gacaccactg ggcatattcg cgacctcgct
     2641 cgaaggatac tctggtttgt ccgctctggg attccaatgg atagccgagt gcagccggtc
     2701 atcatcccca aaaccctcgt gaagatcgat ctcatattcc ttcccgctat cacccggatg
     2761 acggtgcgcg cctgtgagca gccaaatggc cggccacggc cgtccgatcc ctaccggcat
     2821 cttggcgcga gcctcgaaat agaagggagg cttgattcgg agcgcatgaa aactggtgac
     2881 ctggccactt atccacggcg tgccaccacc ttctcgcgcg atagccggta gaatgg
//
LOCUS       DASWQN010001013         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2340308, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001013 DASWQN010000000
VERSION     DASWQN010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340308"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VGC82_17240"
     CDS             complement(<1..226)
                     /locus_tag="VGC82_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEY0425068.1"
                     /translation="MTTVDFAPLFRTAIGFDRLARLVDTAASSQDAQSYPPYNIEKTG
                     DDSYRLTMAVAGFRPDDLELTVKDNTLVVAG"
     gene            complement(389..613)
                     /gene="rpmE"
                     /locus_tag="VGC82_17245"
     CDS             complement(389..613)
                     /gene="rpmE"
                     /locus_tag="VGC82_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY0425069.1"
                     /translation="MKAGIHPEYHEINIIMTDGTQFKTRSCMGKAGDTLRLDIDPKSH
                     PAWTGVQRMMDTGGQVAKFNKKFAGLGIKK"
     gene            829..>1570
                     /locus_tag="VGC82_17250"
     CDS             829..>1570
                     /locus_tag="VGC82_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HEY0425070.1"
                     /translation="MLIRLLVRLVDFSRRHAASVVVGGILLALCAGWVASARLGVSTN
                     TDLLFKQTLPWRQQAMEMDRDFPQFRDLLVAVVDARIPEEADATANELMARLSAEKTH
                     FLTVRRPDANPFLEKEGLLFLPPKQLSALMDQTIDAQPFLGQLVADPSARGLFAALSL
                     LGMGVTQGDADLSPYLAPIKGFHDSMANALAGHAKPLSWEALLGGGLSEMAGPYRFVL
                     AQPKQDFGSLQPGGAATKAMRDIIADLPF"
BASE COUNT          268 a          499 c          468 g          335 t
ORIGIN      
        1 gtccggccac caccaaggtg ttgtccttca cggtcagttc gaggtcgtca ggacggaaac
       61 cagcaaccgc cattgtcagt cgatagctgt cgtcacccgt cttttcaatg ttgtaggggg
      121 gatagctctg cgcatcctgg ctggatgcgg cggtgtccac gagtcgagcc aagcgatcga
      181 aaccgatggc agtacggaac aaaggggcaa aatcaacggt cgtcataaaa gaacctcctg
      241 cgaagcaagg tcgttgttgc aggctgccca tgccgggcca acccattcgt gtcggggacc
      301 ccattgggca tccccgacgt tttctattta ggcaaccctc accgatagtt caagaggtcg
      361 gtcagtgcgg cgctcgtccc gcgtggcgct atttcttgat gccgaggcca gcgaacttct
      421 tattgaattt cgcgacctgg ccgccggtgt ccatcatccg ctgcacgccg gtccacgccg
      481 gatgagactt cggatcgata tccagacgca gcgtatcgcc cgccttgccc atgcacgagc
      541 gtgtcttgaa ctgcgtaccg tccgtcatga tgatgttgat ctcgtggtat tcggggtgaa
      601 tgcccgcctt catggtctga tcccttatct ggcgccaccg atcccgaccg gccgcgcgaa
      661 cgtggcgcta tagcggcccg gcgcggcggg ctcaagcctt gggttcgatc gcttgccagt
      721 ttcggcccct ggcacgcccg tgggtcactt ttaatgcctt ggcggtggac ccaccggggt
      781 ggagcgacta tcacgtcccg cctccatttt gttaggccgt ttcaccgcat gctgatccgc
      841 ctgcttgtcc gtctggtcga tttcagccgc cgccatgcgg catccgtcgt ggtcggcggc
      901 atcctgctcg cgctgtgcgc gggatgggtc gcatcggccc gcctcggcgt cagcaccaac
      961 accgatttgt tgttcaagca aacgctgccc tggcgacagc aagccatgga aatggaccgc
     1021 gattttccgc agttccgcga tttgctggtc gccgtggtgg acgcacgcat ccccgaggaa
     1081 gccgacgcga ccgccaacga actcatggct cggctttcgg cggagaaaac ccattttctg
     1141 accgttcgcc ggccggatgc caatccattc ctggagaagg aaggattgct cttcctgccg
     1201 ccgaagcaac tctcggcatt gatggatcag actatcgatg cccaaccttt cctgggccaa
     1261 ctggtcgccg atccttccgc gcgcggcctg ttcgcggcat tgtcactgct cgggatgggt
     1321 gtgacccagg gcgatgcgga cctgtcgcct tacctggccc cgatcaaggg ctttcatgat
     1381 tcgatggcca acgcgcttgc cggccacgcg aagccgctat catgggaagc cctgctgggc
     1441 ggtggcctga gtgagatggc aggtccttat cgcttcgtgc tggcgcaacc caagcaggac
     1501 ttcggctcac tgcaaccggg cggtgccgcg accaaggcga tgcgcgacat catcgccgac
     1561 ctgccgttcg
//
LOCUS       DASWQN010001014         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_229887, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001014 DASWQN010000000
VERSION     DASWQN010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229887"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..>2033
                     /locus_tag="VGC82_17255"
     CDS             394..>2033
                     /locus_tag="VGC82_17255"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEY0425071.1"
                     /translation="MMDTETTATEPGRDFIRDIVQSDLDAGRTQAVVTRFPPEPNGFL
                     HLGHVKSICLNFGIAREFGGTCHLRFDDTNPIKEEQLFIDSIQRDVSWLGFDWGEHLY
                     YASDYFEQLYEWAEHLVRTGKAYVDDTPPEAMRSQRGTLTEPGQNSPFRYRSIEENLD
                     LFRRMRAGEFPNGARVLRAKIDMASGNMNLRDPVLYRILHTHHPRTGDAWSIYPTYDF
                     AHGQSDAIEHVTHSVCTLEFEDHRPLYDWLIENLPVPSRPRQYEFARLNVSYTILSKR
                     HLTRLVTEKHVRGWDDPRMPTIAGLRRRGVPAASLREFIGRVGVARAYSTVDVAMLDA
                     AIRDTLNPVAPRRMAVLRPLKLIIENYPEGQTETLEARNHPDNPDAGSRTVTFGRELY
                     VEQDDFMEDPPKKFYRLSPGKEVRLRYGYFVTCREVVKDVAGAVVALRCTYDPGSKGG
                     NAPDGRKVQATLHWVAAADAVSAEIRLYAHLFNRPDPNTDGDALADLNPGSLEVLTGC
                     LVEPSLLDAGIGEAVQFERTGYFALDPDSKPGHPVFNRT"
BASE COUNT          393 a          659 c          601 g          380 t
ORIGIN      
        1 ccctcgccta ccgtcacgtt gagctggatg cgctgcggcg ccagatcgcg gctcttcaga
       61 cgtaggcgac cggaccgtcg tgcgacctgc cactatggtc gcgaccgacg gcagcaccgt
      121 ggatgggatt atacgggtac ggcttctgga agtatgagac agcccgttga ttaataacaa
      181 gttgttcaca ggcagcaggg atgacttacg tcgtcaccag cttaggccac tcctgcagaa
      241 acccttggtg acgggacaaa gcaaggagcc gccggatcca ggacttcggc ccgagccttt
      301 tccgatacaa gtcgtcaact cgacaccatt tgtgtagtga gtgccgttcg ctcggccaac
      361 ggccggtttg gcggccattc tggagcctga tgcatgatgg ataccgagac gaccgctacc
      421 gaaccaggac gcgatttcat ccgcgatatc gtgcaatctg acctggatgc cggccggacg
      481 caggccgtgg tgacgcgttt tccaccggaa cccaacggct tcctgcatct ggggcatgtc
      541 aagtcgatct gcctgaattt cggtatcgcc cgggaatttg gcggcacctg ccatctgcgg
      601 ttcgacgaca ccaatccgat caaagaagag cagcttttta tcgactccat ccagcgcgac
      661 gtgagctggc ttggcttcga ctggggcgag cacctgtact acgcctcgga ctatttcgag
      721 cagctgtacg aatgggctga acacttggtg cgaaccggca aggcatatgt tgacgacacc
      781 ccgccggaag ccatgcgctc ccagcgcggc acgctgaccg aacccggcca gaacagcccg
      841 tttcgctacc gttcgatcga ggagaacctg gacctgttcc gccgcatgcg cgctggagag
      901 tttcccaacg gcgcccgcgt gctgcgcgcc aagatcgaca tggcgtcagg caacatgaac
      961 ctgcgggatc cggtgctgta tcgtattctg cacacccacc acccacgcac cggcgacgcc
     1021 tggagcatct atccgaccta cgatttcgcc catggccagt cggacgccat tgagcatgtc
     1081 acccacagcg tctgcacgct cgagttcgaa gaccaccgcc cgctgtacga ctggctgatc
     1141 gaaaacctcc ccgtcccctc ccgcccgcgg cagtacgaat tcgcccgcct caacgtttcg
     1201 tacaccattc tgtcgaagcg ccatctgacc cggttggtga cggaaaagca cgtccgtggc
     1261 tgggacgatc ccaggatgcc gaccatcgcc ggcctccggc ggcgtggcgt cccggcggct
     1321 tcccttcgcg agttcatcgg ccgcgttggc gtcgcccgag cctacagcac cgtggacgtc
     1381 gccatgctgg atgcggcgat ccgcgacacg ctgaatccgg ttgcccctcg gcgcatggcc
     1441 gtgctgcgcc cgttgaagct gatcatcgag aactatccgg aaggccagac cgagaccctg
     1501 gaggcgcgca atcatcctga caatcccgac gccggcagcc ggaccgtgac cttcggccgc
     1561 gaactttacg tcgagcagga cgacttcatg gaggacccgc caaagaaatt ctatcgcctg
     1621 tcgccaggca aagaagtccg cttgcgttat ggctatttcg tcacctgccg ggaggtcgta
     1681 aaagacgtgg ccggagcggt cgtagcattg cgctgcacct atgaccctgg ttcaaaaggc
     1741 ggcaacgcgc ctgatggccg caaggtgcag gcaaccctgc actgggtcgc cgcggccgac
     1801 gccgtatcag ccgaaatccg gctttacgcg catctgttca accgccccga tcccaacacc
     1861 gacggcgatg cgctggcgga tttgaacccg ggatctctgg aggtgctgac cgggtgtctg
     1921 gtggaaccct cgctgctgga tgctggaatc ggcgaagcgg tgcagtttga gcggaccggt
     1981 tactttgctc tggacccgga ttcgaagcca gggcacccgg tgttcaaccg cac
//
LOCUS       DASWQN010001015         1970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1548059, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001015 DASWQN010000000
VERSION     DASWQN010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548059"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VGC82_17260"
     CDS             <1..848
                     /locus_tag="VGC82_17260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0425072.1"
                     /translation="CLSGGRIEMGLVKGAPYEIAPANSNPGRLMRRYWEAHDLIVKAL
                     STTTGPFSWEGEFYQYRAVNIWPRPIQQPTPPIWMTGMSVDTGTAAAERGHVVGTLLS
                     GGLAKPMYEAYRKRARELGWEAGPDRMAYAAIIGVGTTREEGLRRANIIADYVRTAPV
                     VAEAFTNPPGYNSVGANVAMLKAGPRAHRLVTDRNGVPIDHRTATVEQFMDTETVFAG
                     TPDDVFNQLKAFNQRMGGVGHLLFFGQGGHLGHQDAKANISLFGKEVAPRLMELGVPE
                     RAAAE"
     gene            1036..1953
                     /locus_tag="VGC82_17265"
     CDS             1036..1953
                     /locus_tag="VGC82_17265"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425073.1"
                     /translation="MLRRFEALLDPTAGTPAPPPNAGLVRFYWFYIRQVRGLTGALFV
                     CGGLIAVLDTMIPAFIGRIVSLVSSQKPENLFRDSGLTLLGMAAVLLVLRPVSFVAHN
                     ILVNQIVNPGFSNMIRWQNHWHVVRQSWTFFQNDFAGRIANRVLQTGPALRESLVMAF
                     DAAWYILVYGSSAIVLLASLDVRLTLPMILWFALYAAMLCYFVPRLRDRSRRMSEMRS
                     MLTGRVVDSYTNILTVKLFARAVDEDAFVRDAIDEHTAAFRDQTRMTTAYTSTLVVMN
                     ACLIVSMASRTKASSSAARANSLTVRMLV"
BASE COUNT          334 a          656 c          622 g          358 t
ORIGIN      
        1 actgcttgtc gggtgggcgg atcgagatgg gcctggtcaa aggtgcgccc tacgagatcg
       61 cgcccgccaa ctccaacccc gggcggctga tgcgccggta ctgggaagcg cacgacctga
      121 tcgtcaaggc gctgtccacc accaccggcc cgttctcgtg ggagggcgag ttctaccagt
      181 accgcgccgt caacatctgg ccgcggccga tccagcagcc gacgccgccg atctggatga
      241 ccggcatgag cgtcgatacc ggcaccgccg ccgccgaacg cggccatgtc gtcggcacgc
      301 tgctgtccgg cggcctcgcc aagccgatgt acgaggcgta ccgcaagcga gcccgcgaac
      361 ttgggtggga agccggaccc gatcgcatgg cgtatgccgc gatcatcggc gtcggcacca
      421 cccgcgagga gggtcttcgg cgggcgaaca tcatcgccga ttacgtccgc accgcgcccg
      481 tggtggccga agcgttcacc aatccacccg gctacaactc cgtcggcgcc aatgtggcga
      541 tgctgaaggc cgggcctcgc gcacatcgcc tggtgacgga tcgcaacggc gtgccgatcg
      601 accaccgcac cgcgacagtc gagcagttca tggacaccga aaccgtcttc gccggcacgc
      661 ccgacgacgt gttcaaccag ttgaaggcgt tcaaccagcg catggggggc gttggtcatc
      721 tgctgttctt tggccagggc ggacaccttg gtcatcagga cgccaaggcg aatatcagcc
      781 tgttcgggaa agaggtcgcg ccacggctga tggaattggg tgtgccggag cgggccgcgg
      841 ccgagtagcg acgaacaatt gcgagtagcg acaagtcatt gattgtgaca acgtgccccg
      901 ggtgcctggc agtggggcct gataccgcag tggggcctga taccaacgcg tgggccgttc
      961 gtctcgatcg tcgcccggaa acgatcgaga gaggcatgat tgacgaagcc accgacaagg
     1021 cccaccgctg ataccatgtt gcgccgcttc gaagcacttc tcgatcccac cgccggtacg
     1081 ccggccccac cgcccaatgc cggcctcgtc cggttctact ggttctacat ccgtcaggtg
     1141 cgcggcctga ccggggcgct gttcgtctgc ggcggcctga tcgccgtgct ggacacgatg
     1201 atcccagctt tcatcggccg gatcgtcagc ctggtgtcct cacagaagcc ggagaacctg
     1261 ttccgcgaca gcggcctgac cctgctcggt atggccgctg ttctgctggt cctgcggccg
     1321 gtcagcttcg tcgcgcacaa catcctggtg aaccagatcg tcaatcccgg cttctccaac
     1381 atgatccgtt ggcagaacca ttggcatgtc gtgcgccaaa gctggacctt cttccagaac
     1441 gacttcgccg ggcgcatcgc caaccgtgtt ctgcagactg gcccggccct gcgggaaagc
     1501 ctggtcatgg cgttcgacgc cgcctggtac atcctggttt atggctccag cgcgatcgtg
     1561 ctgctggcgt cgctcgatgt tcggctgacc ttgccgatga tcctctggtt cgcgctctac
     1621 gccgcgatgc tgtgctactt cgtgcccagg ctgcgcgatc gatcccgccg tatgtccgaa
     1681 atgcggtcca tgctgaccgg ccgggtcgtc gacagctaca ccaacatcct gaccgtcaag
     1741 ctgttcgccc gagccgtgga tgaggacgcc tttgtccgcg atgcgatcga cgagcacacc
     1801 gcggcgtttc gcgaccagac ccgcatgacc accgcctata cctctacgct cgtggtgatg
     1861 aacgcctgcc tgatcgtgtc gatggcatcg cggacgaagg cgtcctcgtc ggcggcgcgg
     1921 gcgaacagct tgacggtcag gatgttggtg tagctgtcga cgatccggcc
//
LOCUS       DASWQN010001016         3796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2472201, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001016 DASWQN010000000
VERSION     DASWQN010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2472201"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..335)
                     /locus_tag="VGC82_17270"
     CDS             complement(78..335)
                     /locus_tag="VGC82_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0425074.1"
                     /translation="MSILAWLILGLIAGFIASKIVNKTGEGLMLDIVLGVVGAFVGGF
                     LFSFFGATPVTGLNIYSIIVAVIGSIVVLALYHAIAGRRTI"
     gene            complement(380..610)
                     /locus_tag="VGC82_17275"
     CDS             complement(380..610)
                     /locus_tag="VGC82_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425075.1"
                     /translation="MKSLLALVTLVLAALVFEEKARQIAGDARDAYGGAVAQAGDAKR
                     ALTGSVEHQPLISLLIAGGLAYAAATIIPSRN"
     gene            complement(826..2757)
                     /locus_tag="VGC82_17280"
     CDS             complement(826..2757)
                     /locus_tag="VGC82_17280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 61 protein"
                     /protein_id="HEY0425076.1"
                     /translation="MSAADTAHEPPALATASIATTYFEQARLFEQRSCVVLNAALTGH
                     PNGASFNTDCVTTTVRSYTLEDVVLDADSLILFKHGHAIPETAYFEPANAIRQLPDNA
                     SLIELPANEDFVVGCNNAHWGYQHWLTQCVPAIDWALRQQRTRPVRLVLPRLEPWQEQ
                     FLAFLGHDRVPRLTLEPGTRYRLPHVEYSDFLNGTTSFGICLSLLDTIGRMTVVPWLR
                     PAPRILYIHDARPYYGAIRNEGEVLDVLRRCGVTIIEHGRLGVVERINLFRNADAIIG
                     PLGQGLTDVLFCRPGTLLWEWMPRHRQNASFNRLAQAAGVDYWGHLFESVADPAVAGL
                     WEVDLDHLDQGLARLSVRLHEREPKGRGQADTNIVAPRKVEAGPPIDALMLRFESLGD
                     NCEFGLVQRYGGAEPLGLLRFNGFFVPPEFRLQKLVDALNRRFEGLGAPDTVTVFPDG
                     IPGQRELIVRESVYEFWYHTGIAEGTIDPAVQSGHETTRLGFLRRKLLEDLETGEKTW
                     VWKSQATTTRDQLQPLLAALRALGPNTLLWVTEADATHAAGTIEPLERDFVKGYIERF
                     APYEAVTDIDFRSWIEVCRRADECRNPAIALPEQQSEAPPRKLSAMERLAANQPHISA
                     AAASPKARLPPFWRWLSWR"
     gene            complement(2963..>3796)
                     /locus_tag="VGC82_17285"
     CDS             complement(2963..>3796)
                     /locus_tag="VGC82_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEY0425077.1"
                     /translation="RGDATNVVDAPAACAITSISLDHRELLGPTIEIIAGEKAGIMKP
                     GVPVVIGAQPDDVRRVLLQHAQATKAPVLLRGRDWEVEPGANGLRYSDAAGTFDAPVP
                     SLPGRFQQDNAGIALTTLRAAGLRHDAAGLAAIEWPGRLQRLFGNLASLLPADWELWL
                     DGGHNPGAGVVLAEHLRGWSDRPAHLVVGMKQAKDTVEFLRPLLAFAATTWAVREPDQ
                     HSALPVEAIIAASGNVARPGPRVADALRAIPRDGGPARVLICGSLYLAGEVLKLDGDL
                     T"
BASE COUNT          740 a         1167 c         1206 g          683 t
ORIGIN      
        1 cgtcgggatc gccgtcccgc gagaggccga tttgcgcctc cgtacgtcgt aacgtccgga
       61 aagtagccga gtaagaccta gatggttcgg cgtccggcga tggcgtggta gagggccagg
      121 accacgatcg atcctatgac cgccacgatg atgctgtaga tgttgagacc cgtaaccggc
      181 gtcgcgccga agaaggaaaa caggaacccc ccaacgaacg cccccacgac gcctagaacg
      241 atgtcgagca taaggccttc gccagtttta ttgacaattt tgctggcgat gaagccggcg
      301 atcagcccga gaatgagcca agcgaggata gacatgtggt tctccctagg ttgcgttagt
      361 aagactggtt gtgcccctgc tagttgcggc tggggatgat cgttgccgct gcgtaagcca
      421 aaccgcccgc aatcagcaac gagataagcg gctggtgttc gacggatccg gtgagtgccc
      481 gtttggcgtc gccggcttgc gcgaccgccc cgccataggc atcccgggca tcaccggcga
      541 tctggcgcgc tttttcctcg aagacaagag cggccaggac cagggtaacg agcgccagga
      601 gtgacttcat cgattgctcc ttgttccggc tgcggactta ccggatggca gacttggact
      661 ctttagatgg cacgacgcag ccttatcgga aagcgtaacg cgtcggagcg ttctcggatg
      721 cgaaactccg gccgaagcat gatcaaaaag atgaacttgg ggtcctgcca tcctttgcga
      781 cacccgcgcc gcgcaggccg ttcatcagct tatcgcccca ccagcttatc gccaggatag
      841 ccaacgccaa aagggcggta gtctcgcctt gggacttgcc gcggcagccg agatatgcgg
      901 ttggttggcg gccaaacgtt ccatcgccga cagcttcctg ggcggcgcct cggactgttg
      961 ttcaggtaag gcgatggcgg gattgcgaca ttcgtcggca cgccggcata cctcgatcca
     1021 cgacctgaaa tcgatgtcgg tgacagcttc atagggtgcg aaccgctcga tgtagccctt
     1081 tacgaagtcg cgttcgagtg gttcgatcgt gccggctgca tgggtggcat cggcttccgt
     1141 gacccaaagc agggtgttcg gacccaacgc ccgaagggcc gccagtagcg gctgcaactg
     1201 atcgcgcgtg gtcgtggcct gggacttcca cacccaggtc ttttcccctg tttccaggtc
     1261 ttccagcagc ttgcggcgca ggaagcccag acgggtcgtc tcgtgcccgg attgaaccgc
     1321 cggatcgatc gtgccttcgg cgatgccggt gtgataccaa aactcgtaaa ccgactcgcg
     1381 cacgatcaac tcgcgctgac cagggatgcc gtcggggaag accgtcacgg tatcgggcgc
     1441 gccaaggcct tcgaaacgcc ggttcagtgc gtccacgagt ttctgcagcc gaaattccgg
     1501 cggaacgaag aaaccgttga acctgagcag acctagcggt tcggcacccc cgtaacgttg
     1561 cacgaggccg aactcgcagt tgtcccccag gctttcgaaa cgcagcatca atgcatcgat
     1621 cggcggcccg gcctcgactt ttcggggcgc gacaatgttt gtatcggctt gcccacgccc
     1681 ttttggttct cgttcgtgga gtcggaccga taagcgcgca agcccctgat cgagatgatc
     1741 gagatccacc tcccaaaggc cggcaacggc gggatcggct acgctttcga acaggtggcc
     1801 ccaataatcg accccggcgg cctgagccag acgattgaat gaggcgtttt gccgatggcg
     1861 cggcatccat tcccaaagca gcgtccccgg gcggcaaaac agaacatcgg tcaagccctg
     1921 gccgagcggc ccgattatcg catcggcatt gcggaacagg tttatgcgtt cgacgacgcc
     1981 caaacgcccg tgctcgatga tcgtgacccc gcaccggcgc aggacgtcca acacttcccc
     2041 ttcattgcgg atcgcaccgt agtacggcct ggcgtcgtgg atgtagagaa tcctcggcgc
     2101 cggccggagc catggcacca cggtcatgcg tccgatcgtg tcgagcagac tcaggcaaat
     2161 gccgaagctc gtggtgccat tcagaaaatc ggaatattcc acatgcggca gacggtatct
     2221 ggttcctggt tcgagcgtca gtcgcggaac gcggtcatgg cccaggaacg cgaggaactg
     2281 ctcttgccag ggttccaggc gcggcagcac caggcggacg gggcgcgttc gttgttgacg
     2341 caaggcccaa tcgattgccg gcacgcattg cgtcagccag tgctgatagc cccagtgagc
     2401 attattgcag ccgacgacaa agtcctcgtt tgcgggcaac tcgatcagcg aagcgttgtc
     2461 gggaagttgg cggatcgcgt tcgccggctc aaaatatgcc gtctctggga tggcatggcc
     2521 atgcttgaac aggatcaagg aatccgcgtc cagcaccacg tcttccaggg tgtagctgcg
     2581 tacggtcgtg gtgacgcagt ccgtgttgaa cgaagcgccg ttcggatggc ccgtcagcgc
     2641 ggcgttcagg acgacgcaag aacgttgctc gaaaagccgg gcctgttcga aataggtcgt
     2701 cgcgatggac gccgtggcga gggcaggcgg ctcgtgggcg gtatcggcgg ctgacatcga
     2761 ccctacccgt tcgaacactg aacctggcag ccattggcca tgcgcttcat ctggtcaggc
     2821 tgacatgatc cagagcagat tgccgaaggg ccgagagctg cggaggcaaa agtctgccca
     2881 aaacaacgga ttaaaccaca cggcaacccc aaccggaacg ccgtgcgtca tggtaacgag
     2941 aatggatcgc cggttccagc gcttaagtga gatctccatc cagcttcagg acttcgccgg
     3001 ccagataaag actgccgcag atcagcacgc gagcggggcc gccatcccgc gggatcgccc
     3061 gcaacgcatc ggcgacacgc ggtcccggcc gagcgacgtt gccggatgcg gcgatgatgg
     3121 cctcgaccgg aagggcggag tgttgatccg gctcgcggac cgcccaggtg gttgcggcga
     3181 acgccagcag cgggcgaagg aactcgaccg tgtccttggc ctgcttcatg ccaacgacca
     3241 gatgcgccgg ccgatcggac catccgcgca aatgttcggc cagcacgact ccggcgccgg
     3301 ggttgtggcc gccgtccagc cacagttccc aatccgctgg aagcaggctg gcgaggttcc
     3361 cgaaaagccg ctgcagccgg ccgggccatt cgatggccgc cagcccggcg gcgtcgtgcc
     3421 gaaggccggc ggcacgcagc gtggtcaacg cgataccggc gttgtcctgc tggaaccggc
     3481 ccggcaatga ggggacaggc gcgtcgaagg taccggccgc atcgctgtag cgcagaccgt
     3541 ttgcgccggg ttccacctcc caatcccggc cgcgcagcag gacgggtgcc ttcgtcgcct
     3601 gggcatgttg cagcaggacg cggcgtacat cgtccggctg ggcgccgatg acgaccggca
     3661 cgcccggctt catgatcccg gctttttcgc ccgcgatgat ttcgatggta ggtcccagga
     3721 gttcgcggtg gtccagggaa atcgacgtta tggcacaagc ggcgggtgca tcgaccacat
     3781 ttgtggcgtc cccgcg
//
LOCUS       DASWQN010001017         5034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_230201, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001017 DASWQN010000000
VERSION     DASWQN010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230201"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VGC82_17290"
     CDS             <1..430
                     /locus_tag="VGC82_17290"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY0425078.1"
                     /translation="RSALLATIVTAGLVSAASAAAPTRFSAALDGKAEVPPKPGSGFG
                     DALATLDPKTKVLSYTITFQGLSGPATAAHFHGPADAGANAGVAVPIGKDPASPVTGT
                     TTLTDAQIADLEAGKWYVNVHTAANPGGELRGQMKPSMAR"
     gene            complement(491..1045)
                     /gene="thpR"
                     /locus_tag="VGC82_17295"
     CDS             complement(491..1045)
                     /gene="thpR"
                     /locus_tag="VGC82_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY0425079.1"
                     /translation="MRLFVALDLPWMLRDRLAALAGAGLPGARWVPPENYHLTLRFIG
                     ETPRHRAEDIDMALAGLRARGFSLTLAGVGTFAKGGRGTALWVGVERNPQLDHLQNKI
                     ETALQRCGLEPERRRFQPHITLARLDNVPEGKLVSYIQAHSLFRAPPVQVDHFTLFSS
                     QLGKEASVYTAEVEYGLSQSAPVR"
     gene            complement(1050..1667)
                     /locus_tag="VGC82_17300"
     CDS             complement(1050..1667)
                     /locus_tag="VGC82_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY0425080.1"
                     /translation="MMLAPMMLAIAVPARAAPPRLLVLGDSLTAGYGLPHDEGFEFQL
                     QQALKARGHAVTIIDAAVSGDTTAGGRARLDWALSDGADAAIVELGANDGLRGLEPKD
                     MEANLAAILDALAARHIPVLLSGMYAPPNLGPDYQKAFRAVFDRLGSRPGILYDPFFL
                     EGVAMQPSLIQADGLHPNAEGVRHEVSRLLPLVERLLDRIHPPGG"
     gene            1723..2442
                     /locus_tag="VGC82_17305"
     CDS             1723..2442
                     /locus_tag="VGC82_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425081.1"
                     /translation="MDAIPHSPPSAESILPLVRIRDLYLTVPSAAGPVNILRGVGLDI
                     ARGEAVGLVGPSGSGKTSLLMVLAGLERATSGSVSLDGQEITRMNEDALAKLRRERVG
                     IVFQAFHLIPTMTALENVAIPLELAGVRNAMDRARTALDAVGLSHRLTHLPGQLSGGE
                     QQRAALARAFAPEPALLLADEPTGNLDHATGETVIDLLFELRRRTGTTLLLITHDRGL
                     AGRCDRQVHLSDGRVDDPLPA"
     gene            2715..3242
                     /locus_tag="VGC82_17310"
     CDS             2715..3242
                     /locus_tag="VGC82_17310"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY0425082.1"
                     /translation="MIEHRFFAEHFVTPIGTMLLATDAEGHVRALDWEDYAARMHRLL
                     RLQYGTVRLEPKDGVSPARQAMEAYFDGALGAIDSLSVKTGGTEFQRTVWAALRTIQA
                     GTTTTYGNLAVRLGQPRAMRAVGTANGANPVGIIVPCHRVIGANTALTGYGGGIERKR
                     WLLRHEGALPIQDQL"
     gene            3364..4533
                     /locus_tag="VGC82_17315"
     CDS             3364..4533
                     /locus_tag="VGC82_17315"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY0425083.1"
                     /translation="MVQGIHRYPPMDRVIYGIPLEQALADEIGRVDARAVYVMASGTL
                     NRETDVVDTIRRVLGNRLAGMCAKIGSHTPRIDVVAAANEARAARADLIVTIGGGSVT
                     DAAKMVGLCLGNDVAAPGDLDRFRAVISPDGKTERPPVKAPAIRSIDVPTTLSAGEFT
                     SFAGCTDTVRHVKESFFHPLMMPQAVILDPAVTIHTPGWLFLSTGIRAVDHAVEDICS
                     INPTPFSDGTSLQALRLLSDGLRAVKNDPADLAARLSCQLGAWMSIMGSQNGVSKGAS
                     HGIGHVLGGTANVPHGYTSCVMLPHVLRFNQAVNGERQQWVSEALGRPDATAADAVAA
                     LIASLGLPATLRDVGVKPEQLDEIAVGSMHDRWVHTNPRKIDGPPAVRALLDAAW"
     gene            complement(4578..>5034)
                     /locus_tag="VGC82_17320"
     CDS             complement(4578..>5034)
                     /locus_tag="VGC82_17320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0425084.1"
                     /translation="WFPVAPDGASESFAPADSAIQAAAKQGYARVLLVDDDEIVRQVT
                     AELMEASGFAVLSVASAAAALAVVGGDAAVDVLISDLSMPEMDGVSLIKEAQRRRPGL
                     PAILLTGLATNAAEIAAGGTARGFSLLRKPVQGHQLVELVSVLLDAASA"
BASE COUNT          922 a         1651 c         1573 g          888 t
ORIGIN      
        1 acgcagtgct ttattggcga caatcgtaac ggccggtttg gtttccgccg catcggcagc
       61 ggctccgacg cgattttccg ccgcgctcga cggcaaggcg gaggtgccgc cgaaacctgg
      121 ttccggcttc ggcgacgcgc ttgccacgct tgacccgaag accaaggtgc tgagctatac
      181 catcacgttc caaggactca gcggccccgc taccgcggcg catttccatg gcccggccga
      241 cgcgggcgcc aacgccggcg tggcggtgcc gattggcaag gacccggcaa gcccggttac
      301 agggaccaca accctgaccg acgcccagat cgccgatctc gaagccggca aatggtatgt
      361 caacgtccac actgccgcca atccaggcgg ggaattacgc ggccagatga agccttcgat
      421 ggcgcgctga ccctttcata gcgccgaacc tcggcatgat cgcttctgac caacgcgatc
      481 atgccttcgt tcaacggacc ggcgcggact gagacaagcc gtattcgact tccgccgtat
      541 agaccgaagc ctccttgccc aactgcgaac tgaacagggt aaagtggtcg acctgcaccg
      601 gtggggctcg gaaaaggcta tgtgcctgga tgtaggagac cagtttcccc tccggcacat
      661 tgtccaagcg cgccagggtg atatggggct ggaagcggcg gcgttccggt tccaggccgc
      721 agcgttgcaa agcggtctcg atcttgtttt gcagatggtc gagttgcggg ttgcgctcca
      781 cgcccaccca cagcgccgtc ccgcggccac ccttggcaaa cgtgccgacg cccgccagcg
      841 tcagggaaaa gccgcgtgcc cgcagtcccg ccagcgccat gtcgatgtcc tcggcgcggt
      901 ggcggggcgt ttcaccgatg aaccgcaacg taagatggta gttctccggc ggcacccacc
      961 gggcacccgg aagaccggcg ccggccagtg cggccaggcg atcgcgcagc atccacggca
     1021 ggtccagggc gacaaacaac ctcatttcgt caacctcccg gaggatggat cctgtcgagc
     1081 agccgttcca ccaacggcaa cagccgagag acctcgtgcc ttacgccctc ggcattggga
     1141 tgcaaaccgt ctgcttggat caggctgggt tgcatcgcga ctccctccag gaagaacgga
     1201 tcgtaaagta tgccgggacg cgaacccagt ctgtcgaata cggcgcgaaa cgccttctgg
     1261 taatcggggc caagattcgg cggagcatac attccgctca gcaggacggg gatatgccta
     1321 gctgccagtg cgtccaaaat ggcggctaga ttcgcctcca tatccttagg ctccaggccg
     1381 cgcagtccgt cattcgcccc gagttccacg atcgccgcat cggctccgtc gctgagagcc
     1441 cagtcgaggc gggcccgccc gccggcggtg gtgtcgccgg ataccgcggc gtcgatgatg
     1501 gtgacggcgt gcccacgcgc tttcagggcc tgctgcaact gaaactcgaa gccttcgtca
     1561 tgtggcagcc cgtacccggc cgtgagcgaa tctcccagca ccagcaggcg cggcggcgcc
     1621 gcccgcgccg gtacggcgat cgccagcatc atgggcgcca gcatcatcgt cattgcgcac
     1681 agggccttcc gccaccatcc cgcgccacaa tatacagggt cgatggatgc cattccgcac
     1741 tctccgcctt cagccgaatc gatcttgccg ctcgtgcgga tcagggacct gtatttgacc
     1801 gtgccgtccg ccgccggtcc ggtcaacatt ttgcgcggcg tcggcctgga tatcgccagg
     1861 ggcgaggcgg tgggtctcgt ggggccctcc ggatcgggaa agacaagcct gttgatggtg
     1921 ttggcggggc tggaacgcgc cacatccggc agcgtttcgc tggatggcca ggagatcacc
     1981 cggatgaacg aggatgcgct ggcaaaactg cggcgcgagc gggttggtat cgtcttccag
     2041 gcctttcacc tgatccccac catgaccgcc ttggaaaacg tggcgatccc gctggaattg
     2101 gcaggcgtaa gaaacgccat ggaccgagcc cgaaccgctt tggatgcggt cggcctaagc
     2161 caccgcctga cccatcttcc cggccagctt tccggtggtg agcagcagcg tgcggccctt
     2221 gctcgggcgt ttgctccgga acccgcgctg ctgttggccg atgagccgac gggcaacctg
     2281 gatcacgcga ccggggaaac ggtgatcgac ctgctgttcg aactacggcg gcggaccgga
     2341 accactttgt tgctgatcac ccacgataga ggcctcgctg ggcgctgcga ccggcaggtt
     2401 cacctgtcgg acgggcgtgt ggacgaccct cttccggcgt agttctcctg cgctggctgc
     2461 gttccgatcg gttcgcgccc gaccgcatgc cgttcgcgtc cagaagtggc cgacacatgg
     2521 cttgtcgcta gaccatgatc gtttgaggtt gaaccctcca cgacgttcga tcatggtccg
     2581 accttatgtt tgatcgcgtg attcacgcct tctgactggg tcaacctcag gcgatcacgt
     2641 ttaatcgcca acgcgctgct tgccagggcc gaattgtggc gtgcctatgt tgggcagcat
     2701 cttcgggcga gcgcatgatc gagcaccggt tctttgcaga acatttcgtc accccgatcg
     2761 gcacgatgct gctggcgacc gatgccgaag gccatgtgcg tgccttggac tgggaggact
     2821 atgcggccag gatgcaccga ttgctccgcc tccagtacgg caccgtgcga ctggaaccga
     2881 aggatggggt ttcgcccgct cgccaagcga tggaggccta tttcgatggg gcgctcggag
     2941 caatcgattc actatcagta aagaccggcg gcaccgaatt ccaacgcacc gtgtgggcag
     3001 cactgcgcac catccaagcc ggcaccacca cgacatacgg caacctcgct gtccggcttg
     3061 gccagccgag ggcgatgcgc gcggtgggaa cggcgaacgg tgccaatccg gtcggcatca
     3121 tcgtgccgtg ccaccgggtg atcggcgcca acacggcact gaccggctac ggcggcggaa
     3181 tcgaacgcaa gcggtggctg cttcgccatg agggcgcgct tccaatccag gatcagcttt
     3241 agctggtcgg agcggcccac gattagcgcc tgggccgcag tcgcggcatg acccgcgtgc
     3301 caccgctttg accccgcgcc tcgatctccg cacactccct ttaagtcata caggagtgag
     3361 tccatggtcc agggcatcca ccgttacccc ccgatggatc gggtgatcta cggcatccct
     3421 ttagaacagg cgcttgctga cgagatcggc cgcgtcgacg cccgtgccgt ctatgtcatg
     3481 gcgagcggca cgctcaaccg cgagacagat gtagtcgata ccatccgccg ggtccttggc
     3541 aatcgcctgg cggggatgtg cgccaaaatc ggctcacata ccccccgcat cgacgtggtc
     3601 gccgcggcga acgaggcgcg ggcagcaagg gccgacctga tcgtgactat tggcggcgga
     3661 tcggtcaccg atgcggccaa gatggtcggc ttgtgcctgg gcaatgatgt tgccgcgccc
     3721 ggggacctgg atcgattccg agccgtcatc tccccggacg gcaaaacgga acgaccgccg
     3781 gtcaaggcac ccgcaatacg ctccatcgat gttccgacca cattgtcggc cggcgagttc
     3841 acatcgtttg ccggctgcac cgacaccgtt cggcacgtca aggaaagctt cttccatccg
     3901 ctgatgatgc cgcaagcggt gatcctcgac cccgccgtga cgatccacac gcccggatgg
     3961 ctgtttcttt cgacgggaat ccgagctgtc gatcacgcgg tggaagacat ttgctcgatc
     4021 aatccgacgc cattttcgga cggcacgtcg ttgcaggcgt tacgactgct ttccgacggc
     4081 ctgcgcgccg tcaagaatga tcccgccgac ctggccgctc gtttgagctg ccagctcggc
     4141 gcctggatgt cgatcatggg atcgcagaac ggcgtgagca agggcgccag tcacgggatc
     4201 ggccatgtgc tggggggcac cgcgaacgtg ccgcatggct atacctcctg cgtcatgctg
     4261 ccgcatgtac tgcgcttcaa ccaggcagtg aacggcgaac ggcagcaatg ggtcagtgaa
     4321 gcattggggc ggcctgacgc gaccgcggcc gatgcggtcg cggcgctgat cgcaagcctg
     4381 ggtttgccgg cgaccctgcg tgacgtgggc gtgaagccgg agcaactgga tgaaattgcg
     4441 gtcgggtcca tgcacgaccg ctgggtgcat accaatccgc gcaagatcga tggccctccg
     4501 gcggtaaggg cgctgctgga cgcggcctgg tagataattc tgtgatatga tccggaacgc
     4561 ttccacccgg accagatcta agccgacgcg gcatccagca gaaccgacac cagctccacc
     4621 aactgatgcc cctgaaccgg cttgcgcaac aatgagaagc cgcgagcagt gccgccggcg
     4681 gcgatttcgg cagcgttggt cgccaacccc gtcagcagga tggcgggcaa tcccggcctg
     4741 cgccgctgcg cctccttgat cagggacacg ccgtccattt ccggcataga aaggtcggaa
     4801 ataagtacgt ccaccgccgc atcgccaccc acgaccgcca gcgccgccgc cgcggaggct
     4861 accgacagca cggcgaagcc cgaggcctcc atcaattcgg cagtgacctg gcgaacgatc
     4921 tcatcgtcgt caaccagcaa tacgcgggca taaccttgtt tcgcggcggc ctgtatcgcg
     4981 ctgtcagccg gcgcgaagct ttcggacgct ccatccgggg caacgggaaa ccac
//
LOCUS       DASWQN010001018         3364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_493482, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001018 DASWQN010000000
VERSION     DASWQN010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493482"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VGC82_17325"
     CDS             complement(<1..406)
                     /locus_tag="VGC82_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425085.1"
                     /translation="MLDQIQAPIDRDAIAAMDKAGLPDDGPAERFPSRQAAGRGAVTN
                     PNVRFDARAASPFDDGWDTLSLDVDELPRLDTTLTRDSTRSAISWNTSPDIGFDRAVN
                     PYRGCEHGCIYCYARPTHAYLGYSPGLDFETKL"
     gene            complement(565..1794)
                     /locus_tag="VGC82_17330"
     CDS             complement(565..1794)
                     /locus_tag="VGC82_17330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425086.1"
                     /translation="MQHDTADSIESPRSWRAAFLTLAILSLSFGSPLLAVVGLRDMQA
                     AMHTDRSVLSMASALVWIGSGVGGIPMGWLADRIGIRRTVATGTVMMTAGLALSSLGT
                     VWALYVGHGLLIGFLGNGAIYAPLMVYVSRWFDRRRGTALALISSGQYISGMIWPSIL
                     EAGMKHFGWQPVMLAYGIVVLAILPLLLLLRPAPDSLAPGPGQALLRSGDRVLGLHPN
                     IAMVVICAASFCCCVPMAIPASHLVAFCADVGLTAGHGAAMLSVMLACAFISRQFWGL
                     FADRYGGLRTIMTGSAFQMLAIGAFLLTRDEVGLFAIAAAFGFGFSGIIPSYSVVIRD
                     LYPSKEASWRIPTVLMTAMSGMAFGSWFAGRLFDVSLSYRPAFASGVLFNLANLMLIV
                     FLVSRLPSRRLTLAAAS"
     gene            2037..3257
                     /locus_tag="VGC82_17335"
     CDS             2037..3257
                     /locus_tag="VGC82_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY0425087.1"
                     /translation="MTESTGKLPTGPLRGLRVFDLTRVLAGPTCAQMLADLGADVIKI
                     ERPGIGDDTRGFAPPVMPGTQESAYFVGVNRNKQSVTLDIGQPEGQEIALKLIAGCDI
                     LVENFKVGALAKYGLGYEQLHARFPKLVYCSITGFGQTGPYAPRPGYDSLIQAMGGVM
                     SLTGEPEGLPQKVGVPVADLFAGLYGCIGILAALRHRDLTGQGQQIDIGMLDTHVAWL
                     ANQGMNYLATNENPVRLGNQHPNIAPYQVFPTQDGHIVLSVANDPTFKRFCEAFNLTH
                     MLEDPRFITNAARVGNRQLVTDMLTPVMMQHSTTWWVERLEALKIGCGPINKLSEVFE
                     DPHVVARDMVLEMAHSSGHKVKVIANPVKLSETPAEYRLPPPLLGEHTDTVLSDRLGL
                     DAAALAVLREKNII"
BASE COUNT          675 a         1048 c         1076 g          565 t
ORIGIN      
        1 cgagcttggt ttcgaagtcc aggccgggtg agtagcctag atacgcgtgg gtcgggcgcg
       61 cgtaacagta gatgcaaccg tgctcgcagc cgcgatacgg attgaccgcg cggtcgaagc
      121 caatgtccgg ggacgtgttc cagcttatcg ccgaacgggt tgaatcgcgg gtaagcgtcg
      181 tgtcgagcct gggcagctca tcaacgtcca gcgacagcgt atcccaccca tcatcgaacg
      241 gcgatgccgc gcgagcatcg aaccgcacgt tcggattggt gaccgcaccc cgccctgcgg
      301 cctgacgtga gggaaaccgc tcggcgggcc cgtcgtccgg caggccggct ttgtccatgg
      361 ctgcgatggc atcacggtcg atcggtgctt ggatctggtc cagcatggtg gatccgttcg
      421 ctctatgttc acgaccttga tctcatgctg ttggcgccaa gtcaagaaca tgatgcgaac
      481 gtgagcgggc tcggtaacct cgggttggaa gctgctggct gcggtcggga acccgtgctg
      541 aatgtgtccc ttcgggctgc gacctcagga cgcggcggcc agggtcaacc gccggctggg
      601 caagcggctg accaggaaca cgatcagcat caggttggcc agattgaaca gcactcctga
      661 ggcgaaggcc ggccgatagg acagggaaac gtcgaacaat ctcccggcga accagctacc
      721 gaacgccatg cccgacatcg ccgtcatcag caccgttggt atgcgccagg acgcctcctt
      781 cgacggatac aaatcgcgga taacgaccga gtaggatggg ataatgccac tgaagccgaa
      841 cccgaaggcg gcggcgatgg cgaacagacc gacttcgtcg cgcgtcagca ggaatgcgcc
      901 gatcgccagc atctggaatg ccgaaccggt cattatggtg cgcaagccgc cgtaccggtc
      961 ggcgaacaac ccccagaatt gcctcgaaat gaaggcgcag gccagcatca ccgacagcat
     1021 cgctgctccg tggccggcgg taaggccaac atcggcacag aacgccacca ggtgggaggc
     1081 cggaatcgcc atcgggacgc agcagcagaa gctggccgcg cagattacga ccatcgcgat
     1141 gttggggtgc aggcccagga cccggtcgcc cgaccgcagc aacgcctggc cgggaccagg
     1201 cgccagcgaa tcgggcgcag gtcgaagcag cagcaacaac ggcagaatcg ccagaacgac
     1261 aatgccatag gcgagcatga ccggctgcca gccaaaatgc ttcattccgg cctccaggat
     1321 cgaaggccag atcatgccgg agatatattg tccggatgat atcaacgcga gggcggtacc
     1381 gcggcgacgg tcgaaccaac ggctgacata caccatcagc ggggcgtaga tcgcgccgtt
     1441 gccgaggaaa ccgatcagca gcccgtggcc gacgtacaaa gcccatacgg tgccgagact
     1501 ggacaacgcc aggcccgcgg tcatcatcac cgtccccgtg gcgacggtcc tgcggatgcc
     1561 aatgcggtcc gcaagccagc ccattggaat gccgccgaca ccgctgccga tccataccag
     1621 ggcgctggcc atggaaagca ccgaccggtc ggtatgcatc gccgcctgca tgtcgcgcag
     1681 gccgaccacg gcgagcaaag gcgaaccgaa ggacagcgaa aggatagcca gggtcaaaaa
     1741 ggcggcgcgc catgaacgcg gcgattcgat gctgtccgcg gtatcgtgct gcatggatgt
     1801 tccttccccc gccggatttt actgcccgat catcctgcgg gcggttccca aaggttcgtt
     1861 ggcccgggat ctttgcccgg aaccgttggc cccagggtca ttgcccccgg gtcgttggcc
     1921 ccaggatcgt tggcccccgg gtcgttggcc ctggtcgttt gcttgtgcca gggggcgatc
     1981 tataaaccaa tatcgaacca cggccggcaa gatcaaccgg catcttggag atggatatga
     2041 ccgaatcgac cgggaagctg ccgactggtc ccctccgggg ccttcgcgtg ttcgacctga
     2101 cccgcgtcct tgccgggccg acttgcgccc aaatgctggc cgatctgggt gccgacgtga
     2161 tcaagatcga gcggccaggt atcggcgacg atacccgtgg cttcgccccg ccggtcatgc
     2221 caggcactca ggagagcgcc tacttcgttg gcgtcaaccg caacaagcag tcggtcacgc
     2281 tggatatcgg ccagcccgag ggtcaggaga tcgccctgaa gctgatcgcc ggctgcgaca
     2341 tcctggtgga aaacttcaag gtcggcgcgc tggcgaaata cggcctgggc tatgaacagt
     2401 tgcacgccag gttcccgaag ctggtgtatt gctcgatcac cggcttcggc cagaccggcc
     2461 cgtatgcacc gcggccggga tacgacagcc tgatccaggc gatgggcggc gtcatgagcc
     2521 tgacgggtga acccgaaggc ctgccgcaga aggtcggcgt cccggtggcc gatctgttcg
     2581 ccggactgta cggctgcatc gggatcctgg cggcgttgcg ccatcgcgac ctgaccggcc
     2641 aagggcagca gatcgacatc ggcatgttgg atactcatgt cgcctggttg gctaaccagg
     2701 gcatgaacta cctggcgacc aacgagaacc cggttcggtt gggcaaccag catccgaata
     2761 tcgcaccgta ccaggtgttt ccgacccagg acggccacat cgtgctgtcg gtcgcaaacg
     2821 atcctacctt caagcgattc tgcgaggcgt tcaacctgac gcacatgctg gaagacccgc
     2881 gtttcatcac caacgccgcc cgcgttggca accgtcagct tgtgaccgac atgctgaccc
     2941 cggtgatgat gcagcattca acgacctggt gggtcgagcg gctggaggcc ctgaagatcg
     3001 gctgtgggcc gatcaacaaa ctatctgagg tgttcgagga tccgcacgtc gttgcccgcg
     3061 acatggtgct ggagatggcg catagttccg ggcacaaggt gaaggtcatc gccaacccgg
     3121 tgaagctgtc ggaaacgccc gccgaatatc gcctgccccc gccgctgctg ggcgagcaca
     3181 ccgacaccgt gttgtcggac cggttgggtc tggatgctgc cgcgcttgct gtgttgcgcg
     3241 aaaagaacat catctgatgg cggcacgcgc cgcatcgatc cgttatctgc tggcaggcaa
     3301 atttcgcaaa ctggcattgc aggaatgggg cgacgccgac gctccggtgg ttgtctgcgt
     3361 gcac
//
LOCUS       DASWQN010001019         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1813849, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001019 DASWQN010000000
VERSION     DASWQN010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813849"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VGC82_17340"
     CDS             complement(<1..853)
                     /locus_tag="VGC82_17340"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY0425088.1"
                     /translation="MRMLSSLVPALALLVSCSAVAQTAYPDRPIKMIVPLAAASAVDV
                     AARIVTQKMADNMGQQFVILNQPGASGLIGAEQVARAEPDGYTIGGFNDSIMTMVPNL
                     QSKLRWDILKDFEPVSLVATVEWGLIANNQTTYKTAADLIAAAKAAPGKIDYGSGGPG
                     SPQHLAMAMFASAAGISLTHVPYKGATQAATDVAAGQIPVGFQGLGTVTALVRGGQLR
                     LIGVTTDRRLPQFPDVPTVSESGLPGFFFNSWFAILAPAGTPKDVIARLNAEVLKAVG
                     DPEVHRRL"
     gene            1229..>1877
                     /locus_tag="VGC82_17345"
     CDS             1229..>1877
                     /locus_tag="VGC82_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0425089.1"
                     /translation="MPPAVSSPAIKFVKSVREQVSPEEWQTRVDLAACYRLTAMYGMT
                     EMIANHISCRVPGSHDHFLINPYGMLYEEIDASCLIKVDLDGNVIFNASDYGVNAAGF
                     VIHSAIHMARHDVDCVAHTHTPAGMAVSAMECGLLPLAQTSMRFLHIAYHDFEGIADD
                     IGERERLVRDLGDHEAMILRNHGLLVVGRTVPAAFNVLYRLERACQVQVMALSCNT"
BASE COUNT          358 a          603 c          561 g          355 t
ORIGIN      
        1 caagcctgcg gtgaacctcg gggtcgccaa ccgccttcaa tacttccgca ttcagcctgg
       61 cgatgacatc cttcggcgtc ccggccggtg ccaggatcgc gaaccaggaa ttgaagaaga
      121 agccgggtag accggattcc gaaacggtgg gtacgtcagg gaattgcggc aatctcctgt
      181 cggtggtcac cccgatcagt ctgagctggc cgccgcgcac cagcgctgtg acggtcccga
      241 ggccctgaaa gccgaccgga atttggcccg cggcaacgtc ggtcgcggcc tgcgtagcgc
      301 ccttgtaagg cacatgggtc agtgagatgc cggcggcgga ggcgaacatc gccatcgcca
      361 gatgttgtgg gctgcccgga ccaccggagc cataatcgat ctttccaggc gcggccttgg
      421 ccgcggcgat caggtcggca gcggtcttat aggtggtctg gttgttggcg atcagtcccc
      481 actccaccgt cgcgaccagc gacaccggct cgaaatcctt caggatgtcc cagcgcagct
      541 tggattgcag gttgggcacc attgtcatga tgctgtcgtt gaagccgccg atcgtgtagc
      601 cgtcgggctc ggccctcgcc acctgctcgg cgccgatcag cccagaggcg cccggctggt
      661 tcaggatcac gaattgctgg cccatattgt cggccatttt ctgggtaacg atgcgcgcag
      721 caacatccac cgcgctcgcc gccgccagcg gcacgatcat tttgatcggc cggtcgggat
      781 aggcggtctg cgcaaccgca ctgcaactaa ccagcagcgc gagcgctggc accaacgaac
      841 taagcatgcg cataccagaa atcccctcgc taacggaata tgccgcgaat gaccggcgta
      901 tcgatttccg agatgattgc ccctcgcctg cggcttggca atgtgtccca ggacgaaatc
      961 ggaaaaccag ccggagataa aacgccgaag acaatccgat gtccccggga aaggctgttc
     1021 aagagagcct cccacccctt gttttgccct gcagccaaaa acagacacgg tgtcttgccc
     1081 ggcacgccac ctcagcgcag cggcgatggc tgccttgcaa tgatgccgcc gcagccgcct
     1141 tgcggtgcaa ggcgcgttgt aggtatcctc aatctcggcc ggtgaggccc agcttggcga
     1201 attggccctg ttgtaacgga gaaatcccat gccccctgcc gtgagctctc ctgccatcaa
     1261 gttcgtcaaa tcggtccgcg agcaggtgag ccccgaggag tggcaaaccc gcgtcgacct
     1321 cgcggcctgc tatcgcctca cagcgatgta cggcatgacc gagatgatcg ctaaccacat
     1381 ctcatgccgc gttcccggat cgcacgacca tttcctgatc aatccttacg ggatgctcta
     1441 tgaagagatt gatgcctcct gcctgatcaa ggtcgacctc gacggcaacg ttatcttcaa
     1501 cgcctcggac tatggcgtca acgcggccgg cttcgtcatc catagcgcca tccacatggc
     1561 ccgccatgat gtcgactgcg tggcgcacac ccatacaccg gccggcatgg cggtctccgc
     1621 gatggaatgc gggctgcttc cgctggcgca gacgtcgatg cggtttcttc acatcgccta
     1681 tcatgacttt gaaggtatcg ccgacgatat cggagagcgc gagcggctgg tcagagacct
     1741 cggcgatcac gaggccatga ttttgcgcaa ccacggcctg ttggtcgtcg gccgcacggt
     1801 gccggcggcg ttcaacgtgc tgtaccgcct ggagcgtgcc tgccaggtgc aggtgatggc
     1861 gctgtcgtgc aacacca
//
LOCUS       DASWQN010001020         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_493649, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001020 DASWQN010000000
VERSION     DASWQN010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493649"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VGC82_17350"
     CDS             <1..609
                     /locus_tag="VGC82_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0425090.1"
                     /translation="PGLVPLTFSDEHLALMRPLSGMSLDSAVTVDRQTFREATLFTHR
                     GLSGPAVLQISSYWRQGPVSLNLLPACDAAAFLLERKRVRPKAGLASVLAEVLPARLA
                     AAWATEIEGARLADLPDRVLTAVASSLSGWSVTPSGTEGYAKAEVTCGGVDTRDLSSR
                     TMAANKVPGLFVIGEAVDVTGWLGGYNFQWAWASGWCAGEAV"
     gene            complement(617..964)
                     /locus_tag="VGC82_17355"
     CDS             complement(617..964)
                     /locus_tag="VGC82_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY0425091.1"
                     /translation="MTAGVWHPDAGDLIWTDFDPTKGREQAGRRPALVVSSKTFTALT
                     GLAIVCPITSRVRPFPSSVVLPSGLPVAGEILTSHIRSIDTLARSVLYAGASIEPSLA
                     DEVRAKLAAFITI"
     gene            complement(961..1221)
                     /locus_tag="VGC82_17360"
     CDS             complement(961..1221)
                     /locus_tag="VGC82_17360"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY0425092.1"
                     /translation="MNHSIVGRWGKNLAVRFPNEIASEFRLHEGERVEIESGPEQIVI
                     RRARPRYTLEELFAGKSADEWRAIYAGAYDWGPDTGQEIIEE"
     gene            1346..>2084
                     /locus_tag="VGC82_17365"
     CDS             1346..>2084
                     /locus_tag="VGC82_17365"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY0425093.1"
                     /translation="MDALPFDQALDLQVAEILDRCTSCGACAEICPMPAPAGLDTSAP
                     KTLTAGILTILRGGAHEDASRWAAVCSGSGHCIPVCQHGVNPRLMLALARVTDLGHNA
                     PAARRQTGFTRFGAMTKGVRVLSRLQLPPETLARLRGDADDDAAPEVVFYTGCNVMKT
                     PHIVLLALDIMDAMGIRYRVMGGPSACCGVLQFGAGDLATSGRIAYRTIDRLREAAPR
                     ALSWCPTCQIQLSEVVAPGRSDDPFDMK"
BASE COUNT          359 a          664 c          677 g          384 t
ORIGIN      
        1 ccgggcctgg tgccgctgac gttcagcgat gaacacctgg cgctgatgcg tccgctgagt
       61 gggatgtcgc tggacagcgc cgtcacggtg gatcggcaga cattccggga ggcaaccctg
      121 ttcacccacc gtgggctgtc cggaccggcc gttttgcaga tttcatctta ttggcggcag
      181 gggcctgttt cgctgaacct gctgcctgcg tgcgatgccg cggcattcct gctggagcga
      241 aagcgggttc ggccaaaggc agggctggcc agcgtgttgg ccgaagtgct gccggcgcgg
      301 ttggccgcgg cctgggccac cgagatcgaa ggcgcgcggc tggcggattt gccggaccga
      361 gtgctgacgg cggtggcgtc cagtctttcg ggatggtcgg tcacgccatc cggaaccgag
      421 ggttacgcga aagcggaggt gacctgcggc ggtgtcgata cccgggatct gtcgtcccgt
      481 acgatggcgg cgaataaggt gcccggattg ttcgtgatcg gggaggccgt ggacgtgacc
      541 ggatggctcg gagggtataa cttccagtgg gcctgggcga gcggctggtg tgccggcgaa
      601 gcggtctagc gggcgcctaa atcgttatga aggcagccag tttcgcccgg acttcgtccg
      661 cgagcgatgg ctcgatggat gccccggcgt aaaggacgga acgtgcgaga gtatcgatgc
      721 tacggatatg gctggtcagg atttcaccgg cgaccggaag cccggacggc agcacgacgc
      781 tggagggaaa cgggcgaacc cgggaggtga tcgggcacac gatggccaat cccgtcagcg
      841 ccgtgaaagt cttcgacgag acaaccaacg cgggacgacg gccggcctgc tcgcgcccct
      901 tcgtcggatc gaagtccgtc cagatcagat cgccggcatc cggatgccag acgccggcgg
      961 tcactcctcg atgatctcct ggccggtgtc aggtccccag tcatacgcac cggcatatat
     1021 cgcccgccat tcatccgcgg atttccctgc aaacagttcc tccagcgtat atcgggggcg
     1081 ggcccggcgg ataacgatct gttcggggcc ggattcgatt tccacacgtt cgccctcgtg
     1141 cagccggaac tcactggcga tttcattcgg aaagcggacg gcgagatttt tcccccagcg
     1201 gcctacgatg gagtggttca tgagtagtac ctcctgacaa ggatattcct aacatatcct
     1261 gacgccctgt caaattagtt ctgagaaggc ttccgattca cctgtttcag cgtcaacatc
     1321 cgatcagcaa tctggagttc cgacaatgga cgctctgccg ttcgaccagg cccttgatct
     1381 ccaggtggcc gagatcctcg accgctgcac cagttgtggc gcttgcgccg aaatttgccc
     1441 aatgcccgct ccagccggcc tggacacgtc cgcgccaaag accctcaccg ccggcattct
     1501 gaccatcctg cgcggcggcg cccacgaaga cgccagccgc tgggccgcgg tctgctccgg
     1561 cagcggccat tgcattccgg tttgccagca cggggtaaat ccacgcctga tgctggcgct
     1621 cgcccgggtc accgacctcg gccacaacgc tcccgctgcg aggcggcaga ccggcttcac
     1681 ccggttcggc gccatgacca aaggtgtcag ggtcctgtca cgcctgcaat tgccgccgga
     1741 aacgctcgcc cgcctgcgag gagacgccga cgacgacgca gcgcccgagg ttgtctttta
     1801 caccggctgc aacgtcatga agacgccgca catcgtgtta cttgcgctcg acatcatgga
     1861 cgcgatgggc atccgctacc gggtcatggg cggtccgtct gcctgttgcg gcgtcctgca
     1921 gttcggtgcc ggagacttgg cgacatccgg ccgcatcgcc taccgcacca tcgaccggct
     1981 gcgggaagcg gcgccgcgcg cattgtcctg gtgcccgacc tgccagatcc aactgtcgga
     2041 agtggtcgca cccggccgat ccgacgatcc gttcgacatg aaac
//
LOCUS       DASWQN010001021         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1153214, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001021 DASWQN010000000
VERSION     DASWQN010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153214"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VGC82_17370"
     CDS             complement(<1..862)
                     /locus_tag="VGC82_17370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0425094.1"
                     /translation="MISHDPPDNEARFHLLLDTIPFIAFVIAPSGRAQHYNDRFVEYH
                     GYRPGSDKDSRTRLLHPDDRSGLMQARLSGAAAGVEYVVEARLLRHDGVYRWHRIHNK
                     PLLRDRKVIGWLGTAVDIHDVIHAREILERRVGERTVELETVNRRLTAEIRQRQQTEE
                     GLRASEARYRLMYDRTPMALHSVNDEARLIDVNETWLALFDHTRDEVIGRSPSDFMTP
                     DSAARYRDRAWPEMLASRGRLRVVDYQFVTRSGRVFDGRVAASGEFDAAGRFVRSWAA
                     IADVTVEKRAD"
     gene            complement(891..1127)
                     /locus_tag="VGC82_17375"
     CDS             complement(891..1127)
                     /locus_tag="VGC82_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425095.1"
                     /translation="MFYATYTQGDSLPNTNDLPNYQVGKSASDVKQRLESNACALGYD
                     AVSWRETIVAQAFPALAKTASRTPSAAFSARRGL"
     gene            complement(1422..2096)
                     /locus_tag="VGC82_17380"
     CDS             complement(1422..2096)
                     /locus_tag="VGC82_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEY0425096.1"
                     /translation="MSKREILTAIRRGLKRGPLPADQATMLRGRMAAHPRHLIPARSR
                     LPHPEQVDLFVRNVEKEFGSVTRVADLDAVPDAVADYLAAQNLPGRMVMAPHPELRAI
                     PWSARPMLEVREGRAEATDAVSVQHGFAGIAETGTLMLPSGADRPVTLNLLADTEIVV
                     LRASTVVGAYEEGWDRLRSEIEGMPRNVMFITGPSRSADIEQTLELGAHGPRRLHVVL
                     LEDTNP"
     gene            complement(2096..>2374)
                     /locus_tag="VGC82_17385"
     CDS             complement(2096..>2374)
                     /locus_tag="VGC82_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein LutB
                     domain-containing protein"
                     /protein_id="HEY0425097.1"
                     /translation="MRHWREREFERHLNPATIRANLAVWGFFARRPALYRLATRAAAV
                     GLGLFGRKRGRFSKLPLAGGWTEGRDLPAPEGDTFFARYAREQRAGRT"
BASE COUNT          423 a          796 c          705 g          450 t
ORIGIN      
        1 ggtcggcgcg tttctcgacg gtgacgtccg cgatcgctgc ccagcttcgg acgaaccggc
       61 cggcggcgtc aaattccccg gacgcggcca ctcggccatc gaacacacgg ccggaccggg
      121 tcacgaactg atagtccacg acgcgcaacc gtccacggct tgcgagcatt tccggccacg
      181 cccgatccct gtatcgcgct gccgaatccg gcgtcatgaa atcgctcggc gagcggccga
      241 tgacttcgtc tcgggtatga tcgaacaatg cgagccaggt ttcgttaaca tcgatcaacc
      301 gagcctcgtc gtttacggaa tgcagggcca tcggcgtacg gtcgtacatc agccgatagc
      361 gagcttcgct ggcgcgcaat ccttcctcgg tctgctggcg ttgccggatc tcggccgtga
      421 gccgccggtt caccgtttcc agttccaccg tgcgttcgcc gacgcggcgt tccagtatct
      481 cccttgcgtg tatgacgtcg tggatatcga cggcggttcc gagccagcca atgaccttgc
      541 ggtcgcggag caggggtttg ttgtgaatgc gatgccagcg gtacacgccg tcgtgccgta
      601 gcagcctggc ttccacgacg tattcgaccc cagccgcagc gccggacagc cgcgcctgca
      661 tcaggccgga tcggtcgtca ggatgcagca acctggtcct ggagtctttg tcggaccctg
      721 gccgatagcc atgatattcg acgaaccgat cgttgtaatg ctgggcgcga ccactgggcg
      781 cgattacgaa cgctataaag ggaatcgtat ccagcagaag gtgaaaccgc gcctcgttat
      841 ccggaggatc gtggctgatc aaggcagact tttcgttcct tggtgatgaa ttacaggccg
      901 cgccgtgcgg aaaatgccgc tgaaggcgtg cgagacgccg ttttggccag agccggaaat
      961 gcctgcgcca cgatggtttc gcgccaactc acggcatcat agccaagcgc acaggcgttg
     1021 ctttcaagcc tttgtttaac gtcgctcgct gatttgccaa cctgatagtt cggcagatca
     1081 ttcgtgttcg gcagggaatc accttgggta taggtcgcgt aaaacactcc tcgttctacc
     1141 tcgcaaagat ttgcgtaaag tacgcttccg ggctttgtcg ccgtagtcat gcatgtcact
     1201 ccggagaatt agccggattc aggaccggcg aagacttgga tgtcaatttg gcactggaac
     1261 gggcggctga gaggggggca atccgctgat tcatggatca aatttaagcg atatgaccga
     1321 caccagccga tgttctgttg cttcgaagcc acgacaggca tcagcagctt cgctctcggg
     1381 gcaacagccg cgcccgcggg acggtagctg gcgaagcgac cttacggatt cgtatcctcc
     1441 agcaatacga catgcagccg gcgcgggccg tgggcgccca attccagcgt ctgttctatg
     1501 tccgccgagc gcgatggccc ggtgatgaac atcacgttcc ggggcatccc ttcgatctcc
     1561 gaccgcagcc tgtcccagcc ttcctcgtag gctccaacca ccgttgaagc ccgcagcacc
     1621 acgatttcgg tatccgccag caagttcagc gtcaccggcc gatccgcccc gctcggtagc
     1681 atcagcgttc cggtctcggc gatccccgcg aaaccgtgct gcacgctgac ggcatccgtc
     1741 gcctccgcgc ggccctcccg gacctccagc atcggcctgg ccgaccacgg gattgcccgc
     1801 agctccggat gtggcgccat caccatgcgc cccggaaggt tctgtgccgc caggtaatcc
     1861 gccaccgcat caggcaccgc gtccagatcg gcgactcggg tcaccgatcc gaattccttc
     1921 tccacattcc gcacgaacag atcgacctgt tccggatgcg gtagccgcga ccgcgccggg
     1981 atcaagtgcc gcggatgcgc cgccatccgg ccgcgcagca tcgtcgcctg gtccgccggt
     2041 agcgggcctc gcttcaatcc ccgcctgatc gccgtcagga tctcacgctt gctcatcagg
     2101 tacgccccgc gcgctgctcc cgggcgtacc tggcaaagaa tgtgtcgccc tcgggcgcgg
     2161 gcaaatcccg tccctcggtc cagccgccgg cgagcgggag tttcgagaat cgtccccgct
     2221 tgcgcccgaa caagcccaac ccgacggccg ccgcccgggt cgccaggcga tacaaggccg
     2281 gccgccgcgc gaagaagccc cagacagcca ggttcgcccg gattgtcgcc ggattcaggt
     2341 gccgctcgaa ctcccgctcg cgccagtggc gcat
//
LOCUS       DASWQN010001022         1631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1021805, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001022 DASWQN010000000
VERSION     DASWQN010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021805"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VGC82_17390"
     CDS             complement(<1..971)
                     /locus_tag="VGC82_17390"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425098.1"
                     /translation="MSTTAQTLLAQAAALVPALAKREAAANAARSVPDETIADYHRSG
                     ILRVMQPRRFGGQQASFDLFSRIVEILAEGCAASAWVYAVLAEHQWIIACMPEQAQTD
                     VWGDTPHAVAASSLAPRETARHTSEGWRLSGRFPFSSGCRHAQWAIIGARAEDAAGNK
                     LTRYMLVPMRHVEIADDWHALGLRGTGSCSLLLTDVLVPRHRTVLLRDLLDGTTPGSI
                     VLPDYSLLRAPRGYLVPFSLPAVMFTLARRALALVPATLRTRISRGVHDVAASEVVQM
                     RLGEAAAAIETAVLIMHTRRAESIAALETGAAITPEAVARNRRDVTFA"
     gene            1325..>1631
                     /locus_tag="VGC82_17395"
     CDS             1325..>1631
                     /locus_tag="VGC82_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0425099.1"
                     /translation="MKHWRIDDVAWGAFDPGSVDPALVKLVKAAAMVERNGRDYALYL
                     GSVFRDDPDFRSAADYWAEEEVQHGDALAKWATLADPDWDYAAAFDRYRAGYTIKTDA
                     "
BASE COUNT          275 a          527 c          560 g          269 t
ORIGIN      
        1 gcgaaggtga catcccgccg gttacgtgcc accgcttcgg gcgttatcgc ggctcctgtc
       61 tccagcgccg cgatgctttc cgcacgcctc gtgtgcatga tcagcactgc ggtttcgatc
      121 gccgccgccg cctcgcccag ccgcatctga accacctcgg acgcggcaac gtcatgcacg
      181 ccgcgcgaga tccgggtgcg cagcgtcgcc ggcaccaaag ccagcgctcg gcgagccagg
      241 gtgaacatga cggccggcag ggagaacgga accaggtagc cgcggggcgc gcgcagcagg
      301 ctgtagtccg gcaggacgat cgaaccaggc gtggtgccgt ccagcagatc gcgcagcaga
      361 acggttctgt gccgcggaac aagcacatcg gtcagcagca gcgagcagct tccggttccg
      421 cgcagaccca gggcgtgcca gtcatcggcg atctcgacat gccgcatggg aaccagcatg
      481 tagcgcgtca gcttgttccc cgccgcgtcc tcggcccgag cgccgatgat cgcccattgc
      541 gcatggcggc agccggagga gaacgggaag cgtcctgaca gccgccatcc ctcggacgtg
      601 tgccgggcgg tttcccgggg tgccagggag gatgcagcca ccgcgtgcgg cgtgtcgccc
      661 cagacatcgg tctgcgcctg ctccggcatg caggcgatga tccattgatg ttcggcgagc
      721 acggcataga cccaggcgga cgcggcacag ccttcggcca ggatctccac gatgcgggaa
      781 aacaggtcga aacttgcctg ttggccgccg aaacggcgcg gctgcatgac ccgaaggatg
      841 cccgaacggt gatagtcggc aattgtctcg tccggcaccg accgggcggc gttggcggcg
      901 gcctcgcgct tggccaacgc cggaaccagg gcggctgcct gcgccagcag ggtttgcgct
      961 gtggtggaca acgggcgcgt ccttgaggtg cgaggcagat ggttcggcgg atcggacgct
     1021 tcgtcaaggc gaggaccggc ggttccccgt gcgtcatcat ggcgtgcgtc atcatggcac
     1081 ctgtcatcat ggcgtgcgtc ataatggcat cagggcagtc cacggtggtg ggcgtggtat
     1141 gcagcccgcg cgcgttcggg ctgggcggtt ctgtggcaaa atggcatcgc catccggacg
     1201 taacagcgcg ggctgaacca aatgccgctc tgcttggtta cagggcccgt tcggcgttcc
     1261 tattatcccc accgtcgggc gccttccttt ccgcccgctt atcgcaagcg agtactacga
     1321 aaagatgaag cactggcgga ttgacgatgt cgcgtggggt gctttcgacc ccggttctgt
     1381 tgacccggcg ctggtgaagc tggtcaaggc cgcggcgatg gtggaacgca atgggcgtga
     1441 ctatgcgctt tacctcggca gcgtgttccg cgacgacccc gactttcgaa gcgccgcgga
     1501 ctactgggcg gaggaagagg tccagcacgg cgacgcgctg gccaagtggg cgacgctggc
     1561 cgatcccgac tgggattatg ccgccgcatt cgaccgctac cgcgccggct acaccatcaa
     1621 gaccgacgcc g
//
LOCUS       DASWQN010001023         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_230872, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001023 DASWQN010000000
VERSION     DASWQN010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230872"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VGC82_17400"
     CDS             <1..336
                     /locus_tag="VGC82_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425100.1"
                     /translation="TQCGTSLIAAYRGRTPVVMIAGEIVQGAKNKTQSMDQRRFAEAS
                     YARFHTVTSLDNMAEEIAEAFYAARIHRVPVVLNLPMDLQEDDIDWVLFLAPCMISPA
                     IITTGVRPR"
     gene            complement(345..791)
                     /locus_tag="VGC82_17405"
                     /pseudo
     CDS             complement(345..791)
                     /locus_tag="VGC82_17405"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
     gene            721..1716
                     /locus_tag="VGC82_17410"
     CDS             721..1716
                     /locus_tag="VGC82_17410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0425101.1"
                     /translation="MKAGFFVTLEWEAAQDDGRHVPNMLEQVKTARDAGFSSLWLPQH
                     FVSGPNMRQLSTSPVLGLLAGIAEGMTLGTGVLLLPMLNPVLLAEEAATIDRLTGGNF
                     ILGVGLGYRDAEFQAFGVERRSRVPRFREYIEVMRRLWTEERVTFHGKYLHFDDIGLS
                     LRPKNPAGIPIWVGSAVDDGVKRAAEIGDAWISAGAMSRDELTRWWALFHATRQALGK
                     SLDYPRQVARECFCGPDMKTALALAAGPLSAKYARYAGNGWGAFDPSQGTAAFTEFAR
                     DRFVVGDEGFVRDELQRYRDEQGATEFRFRMAWPGVPQEEVLASIRRVGRIAAAL"
BASE COUNT          296 a          559 c          555 g          320 t
ORIGIN      
        1 acgcagtgcg gcacctcgct gatcgccgct tatcgcgggc ggacgcctgt cgtgatgatt
       61 gccggcgaga tcgtgcaggg tgccaagaac aagacccagt cgatggacca gcgccgattc
      121 gccgaggcca gctacgcgag gtttcacacc gtcactagcc tggacaacat ggccgaggaa
      181 atcgccgagg cgttctatgc cgcacggatc caccgcgtgc ccgtggtgct gaacctgccg
      241 atggacctgc aggaagacga catcgactgg gtcttgttct tggcaccctg catgatctcg
      301 ccggcgatca tcacgacagg cgtccgcccg cggtaagccg cgatcagcga ggtgccgcac
      361 tgcgtcagcc ccggaccgca agtcaccatg cagacgccga ccttgccggt ggtgcgggcg
      421 tatccgtccg ccatggacac cgccatcgac tcatgccggg cactgtatgc cttgatggat
      481 gggtcgcggc acaacgcacc ccagagccac atgttgccgt cgcccatcag gccaaaaaag
      541 tcggtcatgc cctcggcctt gagggactgg gcgattgctt cgtaagcctt catgacgttg
      601 ttccccgggt gcggtgcgcg ttcgggtggc atcatgccgc gatgcccgga tgcgcacaat
      661 cgccgctcga ttgaccgccc ccgatgcccg caataggctc gtttgccaaa ggatgccgcc
      721 atgaaagccg gttttttcgt tactctggaa tgggaagcag cccaggatga cgggcgccat
      781 gtcccgaaca tgctggagca agtaaaaacc gccagggacg ccggcttttc ttcgctgtgg
      841 ttgccgcagc atttcgtcag cgggccgaat atgcggcagc tttcgacgtc tcctgtcctc
      901 gggctgctcg ccggcatcgc ggagggcatg accctcggca ccggcgttct tctgctgccg
      961 atgctaaatc cagtcctgtt ggcggaagaa gccgccacca tcgaccgtct gaccggcggg
     1021 aatttcatcc tgggcgtcgg ccttggatac cgcgacgccg aattccaggc tttcggcgta
     1081 gagcgccggt cccgggtgcc gcgttttcgc gagtatatcg aggtcatgcg ccggttatgg
     1141 acggaggagc gcgtcacctt ccatggcaag tatctgcatt tcgacgacat cggcctcagt
     1201 ttgcggccga agaatccggc cggcatcccg atctgggtgg gcagcgcggt tgatgacggg
     1261 gtgaagcgcg ccgctgaaat cggcgatgcc tggatcagcg ccggcgcgat gagccgcgac
     1321 gaactcaccc gttggtgggc gctgttccat gcgacgcggc aggctttggg caaatcgctg
     1381 gactacccac ggcaagtcgc gcgcgaatgc ttctgcggac cggatatgaa gaccgccctt
     1441 gccctggcgg ctggcccgct atcggctaaa tatgcccgat atgccggcaa cggctggggt
     1501 gcgttcgacc cgtcgcaagg caccgccgcg ttcaccgagt tcgcccgcga tcgtttcgtc
     1561 gtgggcgatg agggcttcgt gcgcgacgaa ctgcagcgct accgcgacga gcaaggtgcg
     1621 accgagttcc gcttccgcat ggcctggcct ggtgtcccgc aggaagaagt cctcgccagc
     1681 atcaggcgcg tcggccgcat cgccgccgct ctctgagccg ggccaccgcc
//
LOCUS       DASWQN010001024         3896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1153467, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001024 DASWQN010000000
VERSION     DASWQN010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153467"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /gene="fabG"
                     /locus_tag="VGC82_17415"
     CDS             complement(<1..603)
                     /gene="fabG"
                     /locus_tag="VGC82_17415"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY0425102.1"
                     /translation="MRRALVTGASGALGGAIVRRLAAAGHHVIVHAHQRIDMAQRLAA
                     ELRQQVGSAEAVQFDLVDPAATEEALTRLLADGPIQILVNNAGVHDDAVMPGMTRSQW
                     LHVLDVSLNGFFNVTQPLLLPMIRTRWGRIVSLSSVSAILGNRGQVNYAAAKAGLHGA
                     TRSLALELASRGIMVNAVAPGIIASPMTAQAFPPETIERLV"
     gene            complement(600..1043)
                     /locus_tag="VGC82_17420"
     CDS             complement(600..1043)
                     /locus_tag="VGC82_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425103.1"
                     /translation="MLIDRLGIARLIPHEGDMCLLDGILSWDRSRIRCATATHRAATN
                     PLRRAGRLPVLCGVEYAAQAMALHAALTHAEAKVPNSGGARRGYLASLRSLTCHIERL
                     DQLEGTLIIEAECLAGEAGRMIYRFALRHQDCILLDGRAGVVLEG"
     gene            complement(1037..1846)
                     /locus_tag="VGC82_17425"
     CDS             complement(1037..1846)
                     /locus_tag="VGC82_17425"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEY0425104.1"
                     /translation="MNGGVIRVAVEAVELCGPGLPDWLTSRPVLSGAEAYRAAPTVLE
                     APVQLSAVERRRAIPSVKLALRVGTAAVAQSGRDPAHLSAVFASSGADGETIVAILAA
                     LAAPGREVSPTRFHNSVHNAPSGYWGIAVQSREAVTSVSCYDASFAAGLLEAGVQAVA
                     SGQPTLLVAYDLPYPEPLHAVRSIGASFGTAFVLSPGSSDRAHAELRLRMTATSGTDA
                     TGCWDQGLEALRLGNPAAKSLPLLVMLASRSAGSIRLDLSHGSLDVEVLPC"
     gene            complement(1843..3015)
                     /locus_tag="VGC82_17430"
     CDS             complement(1843..3015)
                     /locus_tag="VGC82_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY0425105.1"
                     /translation="MTAFCIASSLGAGQEATLSALRRGTSGLMPCRFETVDLPTYIGE
                     VQGLDDWRLDGELVRFDCRNNRLAGLALEQDGFKAAVEAAKQRYGATRIGVFLGTSTS
                     GILQTEQAYRRRDADGRLPPELDYAATQSTFSIADFVMTLLGVGGPAFVVSSACASTT
                     KVFATAARMIETGMCDAAIVGGADSLCLTTLYGFNALQLLAPGPCRPFDAARDGLSIG
                     ESAGFALLERPEQAAAGTLHVLGVGESSDAHHMSTPHPGGAGAALSMSRALAAAGLRP
                     EQIDYINLHGTATRVGDAAEDHAVVSLFGEAMPCSSTKGHTGHTLGASGIIEVIIAAL
                     SITHGLLPGSPHTRTRDPQLRCRYVAAARPARIDRVLTNSFGFGGSNASLILGTAG"
     gene            complement(3039..>3896)
                     /locus_tag="VGC82_17435"
     CDS             complement(3039..>3896)
                     /locus_tag="VGC82_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425106.1"
                     /translation="ATKAHAKRLGIYDAIRFHDRVFTDMPPGYTREAFKYEVSIATDY
                     AIGYGRDRYCARVPVSASQMARMVDYLNGLNRPYRDGKAEYEWSVLNDNCIHVAHNAL
                     SAIGFWPVWPTRRSLLIAAFDFPVPKNEFVNIMRRANDMPVKDLLAVWKDKPARRALL
                     AGDGLPTRAGALATSEPVRHDNLLYETELRLIFYDEPLFGHYEEWFRRIESEPRYTDL
                     RADVEYYRALYAQIETERRPLAWWLANHPVLRSEADFPSFYARYYEEIERAVAALSTT
                     GPLFAMAGG"
BASE COUNT          739 a         1283 c         1241 g          633 t
ORIGIN      
        1 caccagccgc tcgatcgttt caggcggaaa cgcctgggca gtcatcggtg atgcgatgat
       61 cccgggtgct acggcattca ccataatgcc tcggctcgcg agttccagcg cgagcgatct
      121 cgtggcaccg tgcaggccgg ccttcgccgc cgcgtagttg acctggcccc ggttgccgag
      181 gatcgccgaa accgacgaca gggaaacaat ccgtccccag cgcgtgcgga tcatcggcag
      241 cagcagcggc tgcgtcacgt tgaagaagcc gttcagcgaa acgtccagca catgcaacca
      301 ctggcttcgc gtcatgcccg gcatgacagc gtcgtcgtga acccccgcat tgttcaccag
      361 gatctggatc ggcccgtcgg cgagcagccg cgtcagcgct tcctcggtcg ctgccggatc
      421 gacaagatcg aattgaacgg cctcggccga accgacttgc tgccggagtt ccgccgcaag
      481 cctctgagcc atgtcaatcc gctggtgcgc atggacgatg acatgatgcc ccgcagcggc
      541 gaggcgccga acgatcgcgc cccccagcgc accgctggcg ccggtgacca gcgcgcgtct
      601 catccctcga gcacgacgcc agcccgtccg tcgagcaaaa tgcaatcctg atgccgcagg
      661 gcaaagcggt agatcatccg gcccgcctct ccggccaggc attccgcctc gataattagg
      721 gtcccctcga gctggtccag ccgctctatg tgacaggtca gggaacgcag gctggccagg
      781 taaccccgtc gcgccccacc cgaattcggc accttcgctt cagcgtgggt cagggcggcg
      841 tgcaatgcca ttgcctgggc agcgtactcc acgccgcaca aaacaggtaa tcgtccggct
      901 cgccgtaacg gattggttgc ggcccggtga gtggcggtag cacaccggat ccgcgatcga
      961 tcccagctca ggatcccatc gagcaggcac atatccccct catgcgggat cagccgggca
     1021 attccgagcc tgtcgatcag cacgggagga cctcgacatc gagggacccg tgactgagat
     1081 caagccggat gctgccggcc gatcgactgg ccagcatgac gagcagaggc agtgatttcg
     1141 cagccggatt gccgagacgc agcgcctcca ggccttgatc ccagcagccc gtcgcatccg
     1201 tgccagacgt ggcggtcatc cgcagacgaa gttctgcgtg cgcacgatcc gatgatccag
     1261 ggcttagcac gaacgctgtc ccgaagctcg cgccgatgga ccgcacggcg tggagcggct
     1321 ccggatacgg aaggtcatag gcgacgagca acgtcggttg cccgctcgcc accgcctgta
     1381 caccggcctc aagcaggccg gcggcgaaac tcgcatcgta gcaactgacg ctggtgactg
     1441 cttcccgcga ttgcacggcg attccccaat agcccgacgg cgcattgtgg accgagttat
     1501 gaaaccgtgt cggcgagact tcgcgcccag gcgcggccag tgccgccagg atggcgacaa
     1561 tggtttctcc atcggccccg gaggaagcga acaccgcgga cagatgggct gggtcgcgcc
     1621 ccgattgcgc aacggccgcg gtcccaaccc gcaatgccag cttgacggat gggatcgccc
     1681 gacgccgctc gacggccgaa agttgcaccg gtgcttccaa caccgtcggc gcagctcgat
     1741 aggcttcggc gccggacagc accggacggc tggtcagcca atccggcagg cccgggccgc
     1801 atagttcgac tgcttcgacg gccacgcgga taacgccgcc gttcatccgg ccgtccccag
     1861 gatcaggctg gcattgctgc cgccgaagcc gaaggaattc gtgaggacac ggtcgatgcg
     1921 cgcgggccgt gccgcggcca cataccggca gcgcagttgc gggtcgcggg ttcgggtatg
     1981 cgggctgccg ggcaacaggc cgtgggtgat cgacagcgcg gcaattatta cctcgataat
     2041 tcccgatgca ccgagcgtgt gtccggtatg gcccttggtg gagctgcagg gcattgcctc
     2101 gccgaacagg gaaacgacgg cgtgatcctc cgccgcatcg ccgacacggg tcgcggtgcc
     2161 gtgcaggttg atatagtcga tctgctccgg ccgcagtcca gcggcggcga gggcccgtga
     2221 catcgacaat gcggcaccgg cgccgcccgg atgtggcgtt gacatgtggt gcgcatcgct
     2281 gctctcgccg acacccagca catgcaatgt gccggcggcc gcctgttcgg gccgctccag
     2341 cagggcgaat cccgcggact cgccaatcga cagcccgtcg cgggcggcgt cgaacggccg
     2401 gcaaggtcca ggggcaagga gctggagcgc gttgaagcca tacagcgtgg tcaggcaaag
     2461 actgtcggca ccgccgacga tcgccgcatc gcacataccg gtttcgatca ttcgtgccgc
     2521 cgtagcgaac accttggtgg tcgaggcaca ggccgaggag acgacaaagg ccggaccgcc
     2581 gacgccaagc agggtcatga caaaatcggc gatcgaaaac gtgctttgcg tggcggcata
     2641 atcgagttca ggtggtagcc gcccatcggc atcgcgacgg cgataggcct gctcggtttg
     2701 caggatcccc gaggtgctgg tgccgaggaa aaccccgatg cgtgtcgccc cgtaacgctg
     2761 tttggcggcc tcgactgcgg ccttgaaccc atcctgttcc agcgcaagtc ccgccagacg
     2821 gttgttgcgg caatcgaatc gaaccagttc cccatccagc cgccaatcgt cgaggccctg
     2881 gacctcgcct atgtatgtcg gcagatcgac ggtttcgaac cggcacggca tcaatccgct
     2941 cgtgccacgg cgcaacgccg acaaggtcgc ttcctgtccg gcgcccaggc tgctggcgat
     3001 gcaaaaagcg gtcatggcga gcggtcgcac cggcagcctc agcccccagc catggcgaaa
     3061 agagggcccg tggtcgaaag ggcagcgacc gcacgttcta tctcttcata ataccgggcg
     3121 tagaacgacg gaaagtcggc ctccgaacga agcaccggat ggttcgccag ccaccacgcg
     3181 agcgggcggc gctcggtctc gatctgcgca taaagcgcgc ggtagtactc aacgtcagca
     3241 cgcagatcgg tatagcgcgg ctccgattcg atgcgacgga accactcctc atagtggccg
     3301 aacaagggct cgtcatagaa gatcaaccgc agttccgttt cataaagcag gttgtcgtgc
     3361 cggacgggtt ccgaagtcgc cagggcgccg gcacgtgtcg gcaggccatc gcccgcgagc
     3421 agcgctcgac gggctggctt gtccttccag accgcgagca gatccttgac cggcatgtca
     3481 ttggcgcgac gcatgatgtt gacgaattcg ttctttggca ccgggaagtc aaacgcggcg
     3541 atcagaagcg accggcgagt cggccaaacc ggccagaaac cgatagccga cagcgcgtta
     3601 tgggcgacat ggatgcagtt gtcgttcaac acactccatt cgtattcggc cttgccatcc
     3661 cggtacggcc ggttcaggcc gttcaggtaa tcgaccatac gggccatctg cgacgcggaa
     3721 accggcaccc gcgcgcaata gcggtcccgc ccataaccga tcgcgtagtc ggtggcgatc
     3781 gagacctcgt atttaaatgc ttcccgcgtg tagccgggcg gcatgtccgt aaacacgcgg
     3841 tcgtggaagc ggatggcgtc gtagatccca aggcgtttcg catgcgcctt ggtcgc
//
LOCUS       DASWQN010001025         4181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_494321, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001025 DASWQN010000000
VERSION     DASWQN010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494321"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VGC82_17440"
     CDS             <1..909
                     /locus_tag="VGC82_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0425107.1"
                     /translation="WLFTMYRGRIRFETPMLWTVSFMVTFVIGGMTGVLVAVPPVDFV
                     LHNSLFLVAHFHNVIIGGVLFGAFAGLEYWFPKAFGFRLDERWGKIGFWFTFIGFYVT
                     FIPLYIAGMLGMTRRMQHYDVAAWQPWMVIAAIGMLLLSIGVISQIAQIVVSIRHREA
                     LRDRTGDPWDGRSLEWATSSPPPVFNFAFYPDVRGEDPYWALKQHAKGQQLEPGGPEY
                     QDIEMPRNSPTGFVCAFFATVMGFALIWHIWWMVILGGIGAFATFVVFAWRDHDEYVI
                     PAAEVARIDRSNIEERRNLVSMAGAL"
     gene            909..1529
                     /locus_tag="VGC82_17445"
     CDS             909..1529
                     /locus_tag="VGC82_17445"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome (ubi)quinol oxidase subunit III"
                     /protein_id="HEY0425108.1"
                     /translation="MAMTATITHRHADPYHLGSTVEHPGPPPKRIVTAYGFWIFLLSD
                     IVMFSCFFAAYAVLVNQTADGPKGSQIFDWTNLAIETVCLLLSSFSCGMASIAADLRN
                     RLWFYLSMAATCVLGLAFLTIEFREFADLVSRGFGPSRSAFLTAFFSLVGCHGLHVSA
                     GVLWLLTMMAQVFAKGFRADIMRRMTCFALFWHALDIIWVAVFSVV"
     gene            1547..1936
                     /gene="cyoD"
                     /locus_tag="VGC82_17450"
     CDS             1547..1936
                     /gene="cyoD"
                     /locus_tag="VGC82_17450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HEY0425109.1"
                     /translation="MSESSEHFIEPLDVAPGDEGEHNLRTRLLGYIVGLGLALLLTAT
                     SFFIAGTHLVWQPSIPVAIIVLAIAQMGVHLVFFLHITTGPDNTNNVLALAFGVLIVF
                     LVVGGSIWIMDHLNQNMPPMDRIMQMQ"
     gene            2055..3332
                     /locus_tag="VGC82_17455"
     CDS             2055..3332
                     /locus_tag="VGC82_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEY0425110.1"
                     /translation="MHWKTRSVANHMVSKRSSMADLSVDAPLLTGRSPSLGINGKVAG
                     VAGCILLGGTLGLGWTISWRQGALYLLGGALGLSLYHALFGFTSAWRVFIADRRGTGL
                     RAQMIMLAVAVVLFFPPISHGVLFGEPVRGEYGAVGIGMMTGAFLFGFGMQLGGGCAS
                     GTLYSAGGGNTRMFVTLAAFIAGATLGAKHLAWWTALPNIGIVSLIESLGMPFALGLS
                     LLLMALIYSATVVLEKRRHGRLIPGAQSGIHGWRRFVQGPWPLVWGALALALGNFATL
                     YLAGRPWGITSALTLWGSKILLIAGVDVAAWPYWQGARAASLQENLFHDVTTVMDFGI
                     MAGALLAAGLAGKFHPQWRIDARSLLGAIVGGLLLGYGARLAYGCNIGAYFSGIASGS
                     LHGWCWLIAAFVGSILGTRARPLFGLSVEYRGR"
     gene            3943..>4181
                     /locus_tag="VGC82_17460"
     CDS             3943..>4181
                     /locus_tag="VGC82_17460"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEY0425111.1"
                     /translation="MQKILQSFSTAAGTWTARPAAFGLVLVYVVLWLIYDRESFNFHG
                     AVAVITLCMTLFIQRSEHRDTQAIQAKLDELLRTQG"
BASE COUNT          711 a         1252 c         1288 g          930 t
ORIGIN      
        1 tggctgttca cgatgtaccg gggacggatc cgcttcgaga cgccgatgct gtggaccgtg
       61 agcttcatgg tcacgttcgt catcggcggc atgaccggcg tgctggtcgc ggtgccgccg
      121 gtggatttcg tgctgcacaa cagcttgttc ctcgtcgcgc acttccacaa cgtcatcatc
      181 ggtggcgtgc tgttcggcgc cttcgcgggg ctcgaatact ggttcccgaa ggcctttggc
      241 ttccgcctcg atgagcggtg ggggaaaatc gggttctggt tcaccttcat tggcttctac
      301 gtcaccttca tcccgcttta tatcgcgggc atgctcggca tgacccgtcg catgcagcac
      361 tacgacgtcg cggcgtggca gccctggatg gtcatcgctg ccatcggcat gctcttgctg
      421 tcgatcggcg tcatcagtca gatcgcgcag atcgtggtca gcatccgtca ccgcgaagcg
      481 ttacgcgacc gtaccggcga tccctgggac ggacgttcgc tggaatgggc gacgtcgtcg
      541 ccgccgccag tgttcaactt cgcgttttat cccgatgtgc gcggcgagga cccctactgg
      601 gcgctgaagc agcacgcgaa gggccagcaa ctcgagcctg gcgggcccga ataccaggac
      661 atcgagatgc cccggaattc gccaaccggc ttcgtctgcg ccttcttcgc cacggtgatg
      721 gggtttgccc tgatctggca tatctggtgg atggtgattt tgggagggat cggtgcgttc
      781 gccaccttcg tcgtgttcgc ctggcgcgac catgacgaat acgtcattcc ggccgcagag
      841 gtcgcacgca tagaccgttc caatatcgag gagcgccgca atctcgtcag catggcggga
      901 gcgctttgat ggcaatgacc gcgacgatca cccaccgcca cgccgacccc taccatctcg
      961 ggagcacggt cgagcatccg gggccgccgc cgaaacgcat tgttactgcc tacggcttct
     1021 ggattttcct gctttccgac atcgtgatgt tctcgtgctt cttcgctgcc tatgcggtgc
     1081 tggtcaatca gaccgccgac ggcccgaagg gttcgcagat attcgactgg acgaatttgg
     1141 cgatcgagac ggtctgcctg ctgctttcga gcttctcctg cggcatggcc agcatcgcag
     1201 ccgatcttcg caacaggctc tggttttacc tgtcgatggc ggccacctgc gtgctggggc
     1261 tggcattcct caccatcgaa ttccgcgaat tcgccgatct ggtctcgcgc gggtttggtc
     1321 cttcacgcag cgcgtttttg accgcgttct tctcgctggt cggatgccac gggctgcacg
     1381 tctcggcggg ggtactgtgg ttgctcacga tgatggccca ggtttttgcc aagggctttc
     1441 gtgccgacat catgcgccgc atgacctgct ttgcgctgtt ctggcatgcg ctcgacatca
     1501 tctgggtcgc ggtgttttcg gtcgtctaat tgctcggagg tgcagcatga gcgagtcgtc
     1561 cgaacatttc atcgagccgc tcgatgtcgc gccgggcgat gaaggcgaac acaacctccg
     1621 caccagattg ctcggatata tcgtcggcct cgggctcgcg ctgttgctga ccgcgacctc
     1681 gttcttcatc gccggaaccc acctggtctg gcagccgagc atccctgtcg ccatcatcgt
     1741 gctcgccatc gcgcagatgg gcgtgcatct cgtgttcttc ctgcatatca caaccggtcc
     1801 cgacaacacc aacaacgtgc tggcgctggc tttcggtgtt ctcatcgtct tcctggtcgt
     1861 aggcggctca atctggatca tggaccattt gaaccagaac atgccgccaa tggatcggat
     1921 catgcaaatg cagtgaggct gcgggcgcat ggcatcggga ttgtggccgc cctgtccgcc
     1981 cgtgcggcgt tccaccgctg aaactaaatc gcacccgtcc ccggccgatc tggaacttcc
     2041 gatgcggtaa tggtttgcac tggaaaaccc gttcggtcgc gaaccacatg gtgtcgaaga
     2101 ggtcctcaat ggctgatttg tccgtcgatg caccattgct aacgggacgg tcgccctcgc
     2161 tcgggatcaa cggcaaggtt gccggggtcg ccggctgcat cctcctaggc gggacgctag
     2221 gcctggggtg gaccatttca tggcggcagg gagctctcta cctgctcggc ggcgcgctcg
     2281 gcttgtccct gtatcatgcg ctttttggat ttacatcggc atggcgggtg ttcatcgccg
     2341 acaggcgcgg taccggcctg cgcgcacaga tgatcatgct tgcggtcgct gtcgtcttgt
     2401 tctttccacc tatcagccat ggggtgctgt tcggtgagcc agtgcggggc gaatatggtg
     2461 ccgtcgggat cggtatgatg acaggcgcat tcctgttcgg cttcggcatg cagttgggcg
     2521 gcggatgcgc ctccggtacg ctctactcgg caggaggggg aaacacgcgc atgtttgtca
     2581 ctcttgcggc tttcatagcg ggcgcaacgc tcggagcaaa gcatcttgcc tggtggacgg
     2641 cgttgccgaa catcggcatc gtctcgctga ttgagagcct cggtatgcca tttgctctgg
     2701 ggctaagcct cctcctcatg gcgctcatct actccgcaac cgtcgtgctc gaaaagcggc
     2761 ggcacgggcg gctgattccc ggagcccaaa gcggtattca cggatggcgg cgtttcgtgc
     2821 agggaccttg gccgcttgtt tggggggccc tggcgcttgc cctcggtaac ttcgccaccc
     2881 tctatttggc cggccgcccc tggggcataa catcagccct gacgctgtgg ggatcgaaga
     2941 tattgcttat cgccggggtg gacgtcgccg cgtggccata ctggcagggc gcgcgagcag
     3001 cctcgcttca agaaaatctt tttcatgatg taacgacggt gatggatttc ggcattatgg
     3061 ccggtgcgct gcttgcggcg gggctcgccg gcaaatttca tccacaatgg cgcatcgatg
     3121 cgcgttcgct gctcggcgcg atcgtgggcg gcttgctgct gggatatggc gcccgcctgg
     3181 catacggatg caacatcggc gcgtacttca gtggaatcgc ttccggaagc ctgcacggct
     3241 ggtgctggct gatcgcggca ttcgtcggaa gcatccttgg cacccgtgcg cgccccctat
     3301 tcgggctcag tgtagagtac cgaggtcgct agggcggttt ctgtcgctga aattaaggta
     3361 acgatcgcgg tcaacctccg cgcctcggcc tcgttatcgc acttgcattc tcgaacatcg
     3421 gctgaccaca gcggcttgcc ggaacctcgc gcttccagct tcgttaagcc gcgaaggaga
     3481 agaacatgac tgatttcaga gacaagcgcg gtggcgcata tgatccggtg acgaaagccg
     3541 ggaacgcgca gccgccgcaa gtagatgaaa acgcaacacc ggaccaaaga gcgcagtggg
     3601 gtgcggacct gcgacctgaa ccgaagcccg gtcctgacca gagctatccg ttgccggagg
     3661 ggttgaggaa agagcgccaa gggccgctca atagagacac gggacgaagg ccgaagtcgt
     3721 aatctgatgt gcggcggtcc cgcccggctt ggcacggcgg aacggatgcc gccgagtaaa
     3781 ttctagtggt gctcttggtg gccaggttgg caagcgaccc aaggtccgcc gatttcggaa
     3841 gcgccgattg tcacaagcaa cagttccaat ttctacatta ccgaacaacg ggcgccaagc
     3901 ttaccgatgc ctcgacgtcg ctgcgatcag cgggcaaact tcatgcaaaa gatcctgcaa
     3961 agcttctcaa cggccgccgg gacatggacg gcacgtcctg ccgcctttgg gctggttctc
     4021 gtttatgtag tgctctggtt gatctacgac cgggagtcct tcaattttca cggagctgtc
     4081 gcagttatca cgctgtgcat gacgctgttt atacagaggt ccgagcaccg cgatactcaa
     4141 gccattcaag cgaaacttga tgaattgctt cgcacgcagg g
//
LOCUS       DASWQN010001026         2842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1419009, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001026 DASWQN010000000
VERSION     DASWQN010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419009"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..464)
                     /locus_tag="VGC82_17465"
     CDS             complement(129..464)
                     /locus_tag="VGC82_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425112.1"
                     /translation="MRRADHPLASRESLSFADPLDFDHIDLHAASSICVRNTRRTRPA
                     NRCGCTSTCPDRAFAVVGAGSMGLRTIPLCNDWAQRELKIVVRDAAQLSSNGRLVLDD
                     LRAAVQRSH"
     gene            complement(1006..1668)
                     /locus_tag="VGC82_17470"
     CDS             complement(1006..1668)
                     /locus_tag="VGC82_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008852120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HEY0425113.1"
                     /translation="MANLKIVGALAISAAFAMASPAMAQRMGGGGGFHGGGGGGFHGG
                     GGGGFHGGAIGGGMRAGGMGGGGGFRGAAVGGGAPRAAFGGGGFRNANASTGVTTSGF
                     AGRSPVVASGGGRNWNNSGWHGGGWRHGYRPGIGFAAGLAAGSALGYGYGGYYGDNYY
                     DTGYYGDSYAYDSGYYDNGDSGYVVSSSDDDPASCAQRYRSYDPASGTYLGYDGQRHP
                     CP"
     gene            complement(1766..2806)
                     /locus_tag="VGC82_17475"
     CDS             complement(1766..2806)
                     /locus_tag="VGC82_17475"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEY0425114.1"
                     /translation="MHLGRYALGGFGLVFVEATGVEEVGLINEHDLGIWNDAQAESFK
                     PLIAFMRRQGTAIGIQLAHGGRKASSQTAMQGMGPLTEANLKAGDKTWQPVGPTAEPV
                     APGWLTPRQLTTEECKAMVGTWAHAARNAVAAGFDTIEIHTAHGYLLASFLSPVSNTR
                     NDEYGGDRAGRMRLPLEIVEAVRREMPDAMPLFVRVSSVDGTVEGWNMDDTVVFAREL
                     KARGVDVIDCSSGGIAGAATAAQVTRSLGFQVPFAERVRNEADIPTMAVGIILEAQQA
                     EAILQNGQADLIAIGRQSQFNPNIALHWAHDLGINTRFEDWSPEYGWWLEKRIRTLEG
                     FATPTGVVTRRG"
BASE COUNT          559 a          944 c          852 g          487 t
ORIGIN      
        1 ggagaacggc gaccgtccct cctcgcaatg cggcttcaag cgtcgacatt gaaatgatgg
       61 ccggttggtg ggtaccttgg gacagagaat cgtactctgc cgcaggtgtc aacaactcgt
      121 cggcaagatc aatgcgaccg ctgcacagcg gcacgcagat cgtccagcac cagcctgccg
      181 ttgctggaaa gctgcgccgc atcgcgcacc acgatcttca gctctcgctg tgcccaatcg
      241 ttgcacagcg gaatcgtgcg caagcccata ctaccggcgc cgaccacggc gaaggcgcgg
      301 tccgggcacg ttgatgtgca gccgcatcga tttgccggcc tcgttcgccg cgtattgcga
      361 acgcagatcg aactcgcggc atgcaggtcg atatggtcga aatccagcgg gtcggcgaac
      421 gagagactct cgcgggacgc cagcgggtga tccgccctgc gcatcggttg ctgcgggtcg
      481 acctgcagga gctatcaaat tggagcctgt ttgaaccagg ggaaacgata ccaaccggga
      541 actcgaaacg cacgtttctt tccaccccgg aaccgaattc gaatctgggg ggccggaacg
      601 ttctcccgca tcccgctttg attctaactt ctggccttca cggcggatac aaccgaacac
      661 gatagaggta agctcatggg aaatgcgcac cgcgacctaa aaaccagccg gcaaatctac
      721 gacgaaagca tcaaggttcg caggcaggtc gatgatggaa agccgaggca gtcggaagac
      781 gacatgcagc gcgaacagtt cgggccccga ggcgtaccgg gcaaaccgga tccggccaaa
      841 atggtcccgc aacgccggaa gaaaacgcca tcggatttcg atcctggcca cacggcgtga
      901 gcgcggccaa aatctgcgcg ctcgcacgag aaatggaaaa taacgaacgg aaaaaggagc
      961 ggccggacga ccgctccttc tcctgtccct atcggcttta aagttttacg ggcagggatg
     1021 acgctggccg tcatatccga ggtaagtgcc cgaagccgga tcgtatgacc ggtagcgttg
     1081 cgcgcacgag gcgggatcat cgtcgctgct cgagacaacg tagccggaat cgccgttgtc
     1141 atagtagcca ctatcgtagg cataactgtc gccgtagtag ccggtgtcgt agtagttgtc
     1201 gccatagtag ccgccatagc catagcccag cgcgctacct gcggcgagac cggcggcaaa
     1261 gccgataccc gggcgatagc cgtgacgcca accgccgccg tgccagccgc tgttattcca
     1321 attgcggccg cctccgcttg cgacgaccgg actgcgaccg gcgaagccgg acgtagtcac
     1381 gcccgtgctt gcattggcgt tacggaaacc gcccccgccg aacgccgcgc gcggtgctcc
     1441 gccccctacc gcggcaccac ggaaaccacc gccgccgccc atgccccctg cgcgcattcc
     1501 gccaccgatg gcgccgccat ggaacccacc accgccaccg ccgtggaacc cgccgcctcc
     1561 gccaccgtgg aacccgccac cgccgcccat ccgctgcgcc atcgcggggc ttgccatcgc
     1621 gaacgcagcc gaaatcgcca gagctccaac tatttttaga tttgccattt caattctcca
     1681 tcttgatgga aatgcaacgg cgggcggatt tcatcgttcc ttcttgatcg aaaattcacg
     1741 gcagtttgag cgcaacagca atctgctacc cgcgacgggt aacaacaccg gttggcgtcg
     1801 cgaagccctc cagcgtccgg atccgcttct ccagccacca gccgtattcg ggcgaccagt
     1861 cctcgaagcg ggtgttgatg ccgagatcgt gggcccaatg cagcgcgatg ttgggattga
     1921 actgcgactg gcgtccgatc gcgatcagat cggcctggcc gttttgcagg atcgcttcgg
     1981 cctgctgcgc ttcgagaatg atgcccaccg ccatggtcgg aatatcagcc tcgttgcgaa
     2041 cgcgctcggc gaacggcacc tggaagccga ggctgcgcgt cacttgcgcc gctgtcgccg
     2101 cacctgcgat gccgccggag gagcagtcga tgacatcgac gccgcgcgct ttcaactcgc
     2161 gcgcaaacac gacggtgtcg tccatgttcc agccttcgac cgtaccgtcg accgacgaca
     2221 cccgcacgaa cagcggcatc gcatcaggca tttcacggcg caccgcctcg acgatctcca
     2281 gcggcagccg catccgcccg gcgcggtcgc cgccatattc gtcgttgcgg gtgttggaga
     2341 ccggcgacag gaacgacgcc agcagatagc cgtgcgcggt gtggatctcg atggtgtcga
     2401 aaccggcggc gaccgcgttg cgcgccgcat gcgcccaggt gccgaccatc gctttgcact
     2461 cttcggtcgt cagttgccgc ggcgtcagcc agcccggggc gaccggctcg gccgtcggcc
     2521 cgaccggctg ccaggtcttg tcgccggcct tgaggttggc ctcggtcaac ggtcccatgc
     2581 cctgcatcgc ggtctgcgac gaagccttgc ggccaccatg ggcaagctga atgccgatcg
     2641 ccgtaccctg gcgcctcatg aaggcgatca ggggcttgaa gctttcggct tgtgcatcgt
     2701 tccagatgcc gaggtcatgc tcattgatga gtccgacctc ctcgacgccg gtcgcctcga
     2761 caaagacgag cccaaacccg cccagcgcat agcggccgag atgcacgacg tgaaatgtgc
     2821 cgcaggtatt tccggggtcg ca
//
LOCUS       DASWQN010001027         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2341656, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001027 DASWQN010000000
VERSION     DASWQN010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341656"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /gene="dnaG"
                     /locus_tag="VGC82_17480"
     CDS             <1..1671
                     /gene="dnaG"
                     /locus_tag="VGC82_17480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY0425115.1"
                     /translation="DGYHCFGCGAHGDAITFVMQTQGLSFLEAVNQLAAEVGLEVPKP
                     TPEAAEAERRRLDVVGVLEAVHAHYQRRLALPEGRVARDYLLGRGLTEQTIARFGLGW
                     AGDRGALTAELTRQGIEVEQLEDAGLIRRDEATGRTFELFSQRVMFPIRDRRGKIVSF
                     GGRTLGSGQPKYVNGPETAVFSKRRTLYGLDMARDGVRNGGTLIVVEGYMDVIATAQA
                     GFTAAVAPLGTALTQEQLEELWRVSPCPVLCFDGDAAGARAAMRAMDLALPMLTPERS
                     LKFATLPAGEDPDSIVRKGGAPKFQSVLDVAQSPSEALYDMVRGETGDATPEKRAALR
                     NRLIEASGRIADKSLAWEYRGALLERFKSQRTVPARQGQRWDKRRQPLGIPPGLRIPR
                     PSPQHENAASERVRILTAILLRHPFLLNDVFAAYNALSMNPSLTRLRDAIEDWAERAE
                     TLDSAGLMDHLTKAGCEYDVAHVLAETPMPLPGCAAEKAMPAEAESGWWHIFGFLNVE
                     HLREEVALAEADAARNLTPDTQRRLTALSEAFNKVRSGDPDGVGFADA"
     gene            1732..>1959
                     /locus_tag="VGC82_17485"
     CDS             1732..>1959
                     /locus_tag="VGC82_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEY0425116.1"
                     /translation="MATKPMVAGDTATAEQDAETTLLDVTAAGVKRLIAKGKERGYIT
                     FDELNAILPPDQNSSEQIEDVMANFSEMGIQV"
BASE COUNT          376 a          602 c          639 g          342 t
ORIGIN      
        1 gacggctacc actgcttcgg ctgcggcgcg cacggcgatg ccattacgtt cgtcatgcaa
       61 acccaggggc tgtcgttcct ggaggcggtc aatcagcttg cggccgaagt cggtctggag
      121 gtgccgaagc cgacaccgga agccgcggag gccgaacgcc ggcgccttga tgtcgttggc
      181 gtgctggagg cggttcacgc gcactaccag cgccgcctgg ccctgcccga aggccgcgta
      241 gcccgcgact atctgctagg tcgcggcctg accgaacaga cgattgcccg cttcggcctg
      301 ggatgggcag gcgatcgcgg tgcgctcacg gccgaactga cgcgccaggg cattgaggtc
      361 gagcaactcg aggacgccgg gttgattcgt cgcgatgagg caaccggtcg tacgttcgaa
      421 ctgttctcgc agcgagtcat gtttccgatc cgcgaccgtc gtggaaaaat cgtcagcttc
      481 ggcggccgca cccttggctc gggccagccg aaatacgtca acggaccgga aaccgcagtg
      541 ttctccaaac gccgcaccct ttatggcctc gacatggctc gggacggagt acggaatggc
      601 ggaacgctga tcgtggttga aggctacatg gacgtcatcg ccacggccca ggccggattc
      661 accgccgccg tcgcaccctt gggcacggcc ctgacccagg aacagcttga agaattgtgg
      721 cgagtctctc cctgccccgt gctgtgcttc gacggcgacg cggccggcgc cagggccgcg
      781 atgagggcga tggatctggc gttgccgatg ctgacgcccg aacggtcgtt gaaatttgcc
      841 acgttaccgg ccggcgagga tccggacagc atcgtacgca agggcggtgc cccgaaattt
      901 cagtcggtgc tggatgtcgc ccagtcgccc tctgaagcgc tttacgacat ggtccgaggt
      961 gaaaccggcg acgcaacgcc ggagaagcgg gccgcgctgc ggaaccggct gatcgaggct
     1021 tccggacgca tcgccgacaa gtcgctggca tgggaatacc gcggcgcgct gctggaacgg
     1081 ttcaaaagcc aacggaccgt gccggcgcgg cagggacaaa gatgggacaa gcgacgccaa
     1141 ccgttgggca ttccccctgg tttgcgcatc ccgcgccctt cgccgcagca tgaaaatgca
     1201 gcttccgaac gggtgcggat cctgaccgcg atcctgctgc ggcatccgtt tttgcttaat
     1261 gatgttttcg cggcctataa tgcgctatcc atgaacccaa gcctgacgcg gcttcgcgat
     1321 gctatcgaag attgggcgga acgcgccgaa actcttgact ctgctggcct gatggaccac
     1381 ctcacaaaag cgggatgtga gtatgacgtg gcgcatgtgc tcgccgaaac gcctatgccc
     1441 ttaccgggat gtgctgccga aaaggcgatg cctgccgagg cggaatcagg gtggtggcat
     1501 attttcggct tcctcaacgt ggaacatctg cgcgaggaag tcgctttggc tgaagccgac
     1561 gccgcccgga acttgacacc ggacacgcaa cgccgcctca cagcgttgag cgaggcgttc
     1621 aataaggttc gcagcggaga tcccgacggt gtcggtttcg ccgatgcata atatttgtgg
     1681 ggctccgcat gcgcgcgcca agccccgtcg gacgaaggag tgccctgagc gatggccacg
     1741 aagcctatgg tcgccggcga taccgccacg gcggagcagg atgccgagac caccctcctg
     1801 gatgtgacgg cggctggcgt caagcggctg atcgcgaaag gcaaagagcg cgggtacatc
     1861 accttcgatg agctgaacgc gatcttgccg ccggaccaga acagctccga gcagatcgaa
     1921 gacgtaatgg ccaatttctc cgaaatgggc attcaggtc
//
LOCUS       DASWQN010001028         4033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1153733, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001028 DASWQN010000000
VERSION     DASWQN010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153733"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VGC82_17490"
     CDS             complement(<1..288)
                     /locus_tag="VGC82_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY0425117.1"
                     /translation="MHPYRTHTCGALRAADAGKDSRLSGWVHAKRDHGGLLFIDLRDH
                     YGITQCVFAAGSNAFAAADAARPESVVTVTGLVVTREAGTVNPKLPTGEIEL"
     gene            complement(455..907)
                     /locus_tag="VGC82_17495"
     CDS             complement(455..907)
                     /locus_tag="VGC82_17495"
                     /inference="COORDINATES: protein motif:HMM:NF013853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 family protein"
                     /protein_id="HEY0425118.1"
                     /translation="MSDRFYERDVLAWSQHQADLLRRIGQGERVNDVDWANVAEEIEG
                     VGLCELHSVEDFLNLILLHLLKLRAWPDSQACGHWRGEIAGFQENLKRRFCPSMRQRI
                     DLGKLYNAAADQMRAGHPGVPLPPENPFSLDHLLNDDINALLQTWSSR"
     gene            complement(984..1724)
                     /locus_tag="VGC82_17500"
     CDS             complement(984..1724)
                     /locus_tag="VGC82_17500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0425119.1"
                     /translation="MLVGQDPVLLLTGASRGIGHATVKRFSSEGWRVLTISRHAFDAR
                     CPWPGGERNHIQLDLSDVDQVRDALPEIKRIAGGRLDALINNAAVSPKHRDGSKITSL
                     QMGYMDWLHIFNVNFFSAILLAQGLSDYLIESKGSIVNMTSIVGSKVHPFASAAYATS
                     KAGLAALTREMANDLGRYGVRVNAVSPGEIDTAILSPGTEEIVQREIPMRRLGKPKEV
                     ADLLFFLCSDQALYLSGSEVHIDGGQRV"
     gene            2298..3143
                     /locus_tag="VGC82_17505"
     CDS             2298..3143
                     /locus_tag="VGC82_17505"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0425120.1"
                     /translation="MQPLFPVPTLDHVVVNVRDRLDDAASLYQRLGFTLTPRGYHTLG
                     SMNHLAMFGTDYFELIATKPGDDSRPEIMESPFGLDALVFGTEDAAATYEALRRNEVP
                     IDPPRQFSRPVEAEGETRDAVFRTTRLPRSATPAGRLYFCQHLTRDLVWRDEWRHHAN
                     GAVGIARAVIAAEEPGILGGLFARMFGATSVRTTDDGCSLIVGLSRFDVVHPGALWEM
                     FGGAAPDSRGRRDYMAAMTLRTLSLDAAETALEAGGIRGVDRIGTSVLVPAAEAFGVT
                     IEFSV"
     gene            complement(3133..>4033)
                     /locus_tag="VGC82_17510"
     CDS             complement(3133..>4033)
                     /locus_tag="VGC82_17510"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0425121.1"
                     /translation="IPGLEKYGFDIDTYEAGQRLNDHIAALPSAEPTPGQFTVLIVGA
                     GLTGIEAAAEMPGKLRAALGPLRSQGARVILADRKPWIGSDMGESARPVIAEALQSLG
                     IETRTGISLAAVDADGATLGTGERIATATIVWCAGMRANALTAQFPVERDGLGRLPVD
                     EFLKVQGLDAEFAAGDTASLLVDGTHRTVMSCQHGRPMGRFAGHNVICDLIGRPMLKL
                     RIDWYTTILDLGPWGAVCTEGWDRKIVASGMEAKRTKETINRERIYPPRSGDRRAILE
                     AAEPVVQPSPLRLHPAGQEAATR"
BASE COUNT          685 a         1323 c         1307 g          718 t
ORIGIN      
        1 cagttcgatt tcgccggtgg gcagtttcgg gttcacggtg ccggcttcgc gggtgacgac
       61 aaggccggtg acggtgacga cgctttccgg ccgcgccgcg tcggccgcgg cgaaggcgtt
      121 ggagccggcg gcgaacacgc actgggtgat gccgtaatgg tcgcgcaggt cgatgaacag
      181 caggccgccg tggtcgcgct tggcgtgcac ccagcccgaa agacgagaat ccttgccggc
      241 gtcggcggcg cggagggcac cacaggtatg agtgcggtaa gggtgcatgg tctggtgtcc
      301 tggcgaaaac tcaacgcgcg gcgccaccgt gggcagccac cgtatcaggg gcggcacaca
      361 agccttggcc ggtcgatcga gtcaagcagt tcggtggatc atgtcgatcg cggcgaccac
      421 gtttcgtggg cggcgggcgg atcggcatcc ctgctcaccg cgacgaccat gtttgcaaga
      481 gcgcgtttat atcatcgttc agcagatgat ccaggctgaa cgggttctcg ggtggaagtg
      541 gaacgccagg gtggcctgcc cgcatttgat cggccgcagc gttatacaat ttgccaaggt
      601 cgatcctttg tctcatcgac ggacagaaac gccgcttcag gttttcttgg aaaccggcga
      661 tctccccccg ccaatggccg caagcctgac tgtcgggcca agcacgaagc ttcaggagat
      721 gcagcaggat caggttcagg aaatcttcga ctgagtgcag ttcgcacaga ccgacgcctt
      781 cgatctcctc ggcgacattc gcccaatcga catcattcac ccgctccccc tgaccaatgc
      841 ggcggagcag atcggcctga tgctgtgacc aggccaggac atcacgctcg taaaaacgat
      901 ccgacatgag atcaatctac cgcaacagga caggccacgc gacggcgttg acggagaccg
      961 ccaagtcaga ccgccagccg gttctaaacc cgctggccgc cgtcgatgtg gacctcggac
     1021 ccgctgaggt aaagcgcctg gtccgagcac aggaagaaca gcaggtccgc gacctctttc
     1081 ggcttgccga gacgccgcat ggggatttcg cgctgcacga tctcctcggt tcccggcgac
     1141 aggatcgccg tgtcgatctc gcctggcgaa accgcgttca cccgcacccc gtagcgcccc
     1201 agatcgttcg ccatttcccg cgtcagcgcc gctaaaccgg ctttcgatgt cgcataggcc
     1261 gcactggcga acggatgcac cttcgacccc acgatggagg tcatgttgac gatgctgcct
     1321 ttcgactcga tcaggtagtc gctcagcccc tgggccagca ggatcgccga gaagaagttg
     1381 acgttgaaga tgtgcagcca atccatgtag cccatctgca ggctggtgat cttgctgccg
     1441 tcgcggtgct tcggggaaac cgcggcgttg ttgatcagcg catccagccg gccgccggcg
     1501 atccgcttga tctccggcag cgcgtcccgc acctgatcga catcggaaag atcgagctgg
     1561 atgtgattgc gctcgccgcc cggccacggg cagcgcgcat cgaacgcgtg gcgggagatc
     1621 gtcaggaccc gccacccttc gctgctgaag cgcttgaccg tggcatggcc gatgccgcgg
     1681 ctggcaccgg tcaacagcag aacagggtct tggccgacta acatgatccg ggtcctttgc
     1741 gccgcgccag ggcttgtaag ggctacacgt cgccgtccat catcgctcca gcctatccgc
     1801 ctgttcgccc gaagcgggaa cgacggtagc tgcgcaagtc cggcgataca tactgtccca
     1861 gcggtccgac gcgagcatat cgcggccagg atcgtacgtc actcggtggc accgcacttg
     1921 accctcccgt gatggcgccg gtccttctcc atagcggcgg gcccccctgg cctgcccttc
     1981 tggcgttgcc cctcgccgta cccgcgccgc gccggcgccg atcggcatgc gcgtgtttcc
     2041 tcccggtgcg gatttatccg gaacggacgg cggttggcct ttcgcggcca gcggcgtggg
     2101 ttgcggccga tcgcgaatac cagccctgtg gcatcacatc acctcctggc cgcccgctgg
     2161 cggtatcgag gcggaaacgc cggcaacctc atgccgacaa cctcatgccg gcaacctaat
     2221 gatcgtcagc cgcattatcc ttgccccgtc ggccattgtc ggacgaaggt ggccgccact
     2281 aatggaaatg gtaccgaatg cagcctttgt ttcccgtccc tacactggac cacgtcgtag
     2341 tcaatgtccg cgatcgcctg gacgatgccg ccagcctgta tcaacggctg ggcttcacct
     2401 tgaccccgcg cggctaccac acgcttggct cgatgaacca cctggcgatg ttcggcaccg
     2461 actacttcga gttgatcgcc accaagcccg gcgacgacag caggcccgag atcatggagt
     2521 ccccgtttgg cctggacgcc ctcgtgttcg gcaccgagga tgccgccgcg acctacgaag
     2581 ccctgcggcg gaacgaggtc ccgatcgacc cgccgcggca gttctcgcgt ccggtggagg
     2641 cggagggcga aacccgagac gcggtgttcc gcaccacccg cctgcctcgt tcggcaaccc
     2701 ccgccgggcg gttgtatttc tgccagcacc tgacccgaga cctggtctgg cgcgacgaat
     2761 ggaggcatca cgcgaacggc gccgttggca tcgcccgagc ggtgatcgcg gcggaggagc
     2821 cgggaatcct tggcggcctg tttgcccgca tgttcggcgc gacatccgtc cgcaccaccg
     2881 acgacggatg ttcgctcatc gtgggcctgt cgcgcttcga cgtggtgcac ccgggcgcgc
     2941 tgtgggaaat gtttggcggg gccgcgccgg atagtcgggg acggcgcgac tacatggcgg
     3001 cgatgacact gcggaccttg tcgctggacg cagccgaaac cgcgctggag gccggcggca
     3061 tccgcggcgt ggatcgtatc ggcacaagcg tgctggtgcc ggcggccgag gcgttcggcg
     3121 tcaccatcga gttcagcgtg tagcagcctc ctggccggcc ggatgaaggc gcaaaggcga
     3181 cggctgcacg actggttcgg ccgcttccag gattgcgcgc cggtcgccgg aacggggcgg
     3241 ataaatacgc tcgcggttga tggtttcctt ggtgcgtttg gcttccatgc cgctggcgac
     3301 gattttgcgg tcccagcctt cggtgcagac cgcgccccag ggtccgaggt ccaggatcgt
     3361 ggtgtaccag tcgatccgca gtttcagcat ggggcggcct atcaggtcgc aaatcacgtt
     3421 atggccggcg aagcggccca tcggccggcc atgctgacag gacatcaccg tccggtgcgt
     3481 cccatccacc agcaatgacg ccgtatcgcc ggcggcaaat tccgcatcca gtccttggac
     3541 cttgaggaat tcgtccaccg gcaggcggcc gagcccgtcg cgctcgaccg ggaattgtgc
     3601 cgtcagtgca ttggcgcgca tgcccgcaca ccagacgatt gtcgcggttg caatccgttc
     3661 gccggtgccc agggtggcgc catcggcgtc cacggctgcg agcgaaatgc cggtgcgggt
     3721 ttcgatgcca agggactgaa gcgcctcggc gatgaccggc cgagcgcttt cacccatgtc
     3781 ggagccgatc caaggcttgc ggtcggccag gatgacccgc gcgccttggc tgcgcagcgg
     3841 tccgagggcg gcgcgcagct tgccgggcat ctcggccgcc gcctcgatac cggtcaggcc
     3901 ggcgccgacg atcaaaacgg taaactgccc gggcgtgggt tcggcggacg gcagcgccgc
     3961 gatatggtcg ttgagccgtt gtccggcctc gtaggtatcg atatcaaaac cgtatttttc
     4021 cagaccgggg ata
//
LOCUS       DASWQN010001029         4739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1682322, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001029 DASWQN010000000
VERSION     DASWQN010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682322"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VGC82_17515"
     CDS             complement(<1..443)
                     /locus_tag="VGC82_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425122.1"
                     /translation="MANSRFRLGAVLGINQTLSWGMTFYLPAIVAGPAETTFGQSSFA
                     VLGAFSWSLLVTGACAPRVGKWIDQHGGRGALLVSIVVMAMGQAVLAFSNGLALWYLG
                     WTIVGIGMSMGLYDAAFATVGGLLGKEAGPSITGITLLAGFASTV"
     gene            551..1405
                     /locus_tag="VGC82_17520"
     CDS             551..1405
                     /locus_tag="VGC82_17520"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0425123.1"
                     /translation="MSSQSFDYTVGDFNYIEHADGPQTLRLFMPLGAGPFPLVVDLHG
                     GAWCNGDLKDCEPRDLTLVRAGFAVAALNFRHAGAGYPASLVDINYAIRWLKAHAGKL
                     NLRADAVGLSGQSSGGHLAMLAAMRPADPRYTAQASPDGAAVDASVKAVVMQWPVINP
                     LSRYRNALRLKQSGNPPAWVGDIPSRHDLYWKTEAAMEEGNPLLLLERGEKVVLVPAL
                     WIQGRPDPTHDYRDPDGTFDGNEPERFAHNYRKAGGDLELLYVDNATRGTETSHNPTA
                     AFFQRHLT"
     gene            complement(1550..2950)
                     /locus_tag="VGC82_17525"
     CDS             complement(1550..2950)
                     /locus_tag="VGC82_17525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425124.1"
                     /translation="MQRALAAPAIRVKNPVWPLLIVGLGTLVVPLDSAVNIDFSAIVA
                     RFGLPIPMIQWVVIAYVLTQTSLMLTFGRVGDMLGYRRVFLFGTATSTIAFVACALAP
                     TYPALLAGRVLQGIGAGLILSCGPALATSLFPEAMRTQILARYMMMFGIGSTLGPSVF
                     GTLVERFGWSAVFSFRAPIAAAAFALAWTLPRPARGEREPFDAAGGGLLALALGFLLL
                     ALNRLRPPGPETALFAAFAVAGFALFYRQEQLAAKPIIDFSYFREADFAVINLSNALI
                     NLSAFTIMLLAPFYLSRIPGLSLPEAGWVLAASPFGTILAAPMAGRLAKGHKPRLIAL
                     IGTALSGIGLFGISFSGSTPDIPILVMSMLVQGIGLGLFQVAYLDIVTAAIPAHSRGV
                     AGALGMATRTIGTVTGATVLMLIFQGMHAGDPGGFMTAFQATFRIAAAIPAALILLEV
                     RRYRGRKASTRRDDPR"
     gene            3133..3495
                     /locus_tag="VGC82_17530"
     CDS             3133..3495
                     /locus_tag="VGC82_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425125.1"
                     /translation="MSNVQNFPTHLRGLSFSIAELTLIDGWAEARGLVMIVRLDLASA
                     TQEYEEVVALHPERRQPCRWMMWRDTEAVFVRPLVGRTRRHGSVAEAIESLTPLHDGV
                     VTDVMAPRWPDRGFLQDP"
     gene            complement(3449..>4739)
                     /locus_tag="VGC82_17535"
     CDS             complement(3449..>4739)
                     /locus_tag="VGC82_17535"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0425126.1"
                     /translation="NLPAIALHVRAVLTNTTPIGVTRGPGFGEAVNIMERLIDAAATQ
                     CGFDRLDLRRRNFVTVLPMTNAFNFVVDSGDFHAHFERAIGNADMANFPARRRASEAA
                     GRRRGFGLACHIKATGGLPTENVDIRFERDGSVSLITGTQTIGQGHETTMPQILADRL
                     GLPNSLINLRQGDTDLIAMGGGHGSSRATYMGGTAIWRASEIIIAKGLKLAAQALEAA
                     EADIVFEDGDFRVAGTDRTVALLALAAHAPGTLDTYHQWTREAMTFPNGTHIAEVEVD
                     PETGAVTLERLTAVDDYGIIVNPMIAAGQAHGAIAQGAGQALLEHAAYDPVSGQPISG
                     SFMDYAMPRAGDLPSFDLSFAGTRCTTNPVGVKGCGEAGAVALLPAVANAIADAVAVT
                     SQDGPASGNRIWHAMRSPDPASLKGPGGNRGPASAVP"
BASE COUNT          869 a         1513 c         1600 g          757 t
ORIGIN      
        1 aacaccgtgc tcgcgaatcc ggccagcaag gttatcccgg tgatgctggg ccccgcctcc
       61 ttgcccagca ggccgcccac cgtggcaaac gcagcatcgt acaggcccat cgacatgccg
      121 atgccgacga tcgtccaccc gaggtaccag agcgccagcc cgttgctgaa tgccagcacc
      181 gcctggccca tcgccatcac cacgatgctg acaagcagcg cgccccgccc gccatgctgg
      241 tcgatccatt tcccgacccg aggcgcgcag gcaccggtca ccagcaacga ccaggagaat
      301 gcccccagga cggcgaaaga cgattgcccg aatgtcgtct cggcgggacc ggcgacgatc
      361 gcaggcagat agaacgtcat tccccaggac aatgtctggt tgatcccaag cacggcgccg
      421 aggcggaaac gggagtttgc catccggcgc agcatccggc ggatggcgat gcgcaacaac
      481 ccacgacgtt gccggttgtc tgcatcggct tgcggtaata agatgaacga aaatcaaggg
      541 agccaatgcc atgagcagcc agtccttcga ctacaccgtc ggcgatttca actacatcga
      601 acatgcggat ggcccgcaga cgctgcggtt gttcatgccg ctgggagcgg ggccgttccc
      661 actggtcgtc gatctgcatg gcggcgcctg gtgcaacggc gacctgaagg actgcgagcc
      721 gcgcgacctg acgctggtgc gcgccggatt cgcggtcgcc gcgttgaatt tccgtcatgc
      781 cggcgccggc tatccggcct cgctggtcga tatcaactac gcgatccgct ggctgaaggc
      841 acacgccggc aagctcaatt tgcgtgccga cgcggtcggg ctgtcgggtc agagcagcgg
      901 cgggcacctg gcgatgctgg cggcgatgcg tccagcggat ccgcggtaca ccgcgcaggc
      961 ctcgcccgac ggggcggcgg tcgatgcctc ggtgaaggcg gtggtcatgc agtggccggt
     1021 gatcaatccg ctgtcgcgct accgcaatgc gctgcgcctg aagcaatccg gcaatccgcc
     1081 ggcctgggtc ggcgacattc ccagccggca tgacctgtac tggaagaccg aagccgcgat
     1141 ggaggaaggc aacccgctgc tactcctgga gcgcggggaa aaagtcgtcc tggtgccggc
     1201 gctctggatc cagggccggc ccgacccgac gcacgactac cgcgatccgg acggcacgtt
     1261 cgacggcaac gaaccggaac ggttcgcgca caactaccgc aaggccggcg gcgatctgga
     1321 gctgctgtat gtcgataacg ccacgcgcgg cacggaaacg tcgcataatc cgacggctgc
     1381 cttcttccag cgtcatctga cctgacaccg atccgctgaa agacccacac atcggcatca
     1441 ggctgccaac gtcatggtgg ccgaaggcca tcatccacgg ttttcgtgaa acgcgcggaa
     1501 ggcgtggatg ccggacttcg tcggcatgac gaggtcgggc ggcgcgaaat cacctcggat
     1561 cgtcccggcg cgttgatgct ttccggccgc ggtaccgccg gacttccagg aggatcaacg
     1621 cggcgggaat ggccgcggca atccgaaacg tcgcctggaa ggcggtcatg aacccgccgg
     1681 gatcgcctgc gtgcatgcct tggaaaatca gcatcagcac cgtcgctccg gtgacggtgc
     1741 cgatggtgcg ggtcgccata cccaacgctc cggcgacgcc tcggctgtgt gcggggatgg
     1801 cggcggtgac gatgtcgagg taggcgacct ggaacaagcc cagcccgatg ccctggacca
     1861 gcatcgacat caccaggatc gggatgtccg gcgtgctgcc ggagaaagag attccgaaca
     1921 agccgatgcc gctgagcgcg gtgccgatca gggcgatcag gcgcggcttg tggcccttcg
     1981 cgaggcgtcc ggccatgggc gcggcgagga tggtgccgaa cggcgatgcc gccagcaccc
     2041 atccggcttc gggcaaagac aggccgggga tgcggctcag gtagaacggc gccagcagca
     2101 tgatggtgaa cgccgacagg ttgatcagcg cattgctgag gttgatgacc gcgaagtccg
     2161 cctcgcggaa gtagctgaaa tcgatgatcg gtttcgccgc aagttgttcc tgccggtaga
     2221 acagggcaaa tcccgccacg gcgaatgcgg cgaacaaagc ggtctccggc cccggcggcc
     2281 gcaggcgatt gagcgccagg agcaggaaac ccagggccag cgccagcagg ccgcctcccg
     2341 cggcgtcgaa cggttcgcgt tcgccgcgcg ccggccgagg caacgtccac gccagtgcaa
     2401 acgccgccgc cgcgatcggt gcccgaaaac tgaacaccgc gctccagccg aaacgctcga
     2461 cgagagttcc gaagaccgac ggacctaaag tgctgccgat cccgaacatc atcatgtagc
     2521 gagccagaat ctgcgtgcgc atggcctcgg ggaacaggct ggtggctagc gccggcccgc
     2581 atgacaggat cagcccggcg ccgatgccct gcaggacgcg tccggcgagc agcgcgggat
     2641 aggtcggcgc gagtgcgcag gcgacgaagg cgatggtgct ggtggcggtg ccgaacagga
     2701 acacccggcg gtagccaagc atgtcgccga cccggccgaa ggtcagcatc agcgaggtct
     2761 gcgtcaggac ataggcgata acgacccatt ggatcatcgg gatcggcagg ccgaagcgcg
     2821 cgacgatggc ggaaaaatcg atgttgacgg ctgagtcgag aggcacgacc agcgtgccca
     2881 ggccgacgat cagcaggggc cagaccggat tcttcacccg tatcgcgggg gcggcgagcg
     2941 cgcgctgcat cagtttcctc gccattcccg cgagcataca agacaccggc tatcggctaa
     3001 acccgaccca gcgtgatttg cggcgatgac gcggatttcg gaaatatggc agaagtgtgg
     3061 ttcaagtcac actcggatca cgatagaagg tctaagctct cctcgttcac ccccagatgg
     3121 aacgagggta ccatgtccaa cgtccagaac tttcccaccc acttgcgcgg cttgtcgttc
     3181 agcatcgctg aactgacgct gatcgatggc tgggccgagg cgcgtggcct ggtcatgatc
     3241 gtccgcctgg accttgcgtc ggcaacccag gaatacgaag aagtcgtcgc ccttcatccc
     3301 gagcggcggc agccgtgccg gtggatgatg tggcgcgaca cggaggcggt gttcgtgcgg
     3361 ccgctggtcg ggcgaacccg acgccacggg tccgtcgccg aagcgatcga gtcgctgacg
     3421 ccgctgcatg atggtgtcgt gacggatgtc atggcaccgc gctggccgga ccgcggtttc
     3481 ctccaggacc cttgaggctt gcggggtccg gtgaccgcat cgcgtgccag atgcgattgc
     3541 cggaggctgg gccgtcctgg ctcgtgaccg ccaccgcgtc ggcgatggca ttggcgacgg
     3601 ccggcaacag ggccaccgca ccggcctcgc cgcagccttt cacgccgacc ggattggtgg
     3661 tgcagcgtgt gccggcaaaa ctgaggtcga agcttggaag gtcgccggcg cgcggcatgg
     3721 cgtagtccat gaaactgccg ctgatcggct ggccggagac agggtcataa gccgcgtgct
     3781 ccagcaacgc ctgccccgcg ccctgggcga tcgcgccatg cgcctgcccg gcggcgatca
     3841 tcgggttgac gatgatgccg taatcgtcaa ccgcggttag ccgttccaat gtcacggcgc
     3901 cggtttccgg atcgacttcg acctcggcga tgtgggtgcc gttgggaaac gtcatcgcct
     3961 cccgcgtcca ctggtgatag gtatccagcg tgcccggcgc atgggccgcc aatgccagca
     4021 gcgcaacggt gcggtcggtg ccggcgacgc ggaagtcgcc atcctcgaac acgatgtcgg
     4081 cttcggcggc ttccagtgcc tgggcggcca gtttcaggcc ttttgcgatg atgatctccg
     4141 acgcgcgcca gatcgcggta ccccccatgt aggtggcgcg cgagctgcca tggccgccgc
     4201 ccatcgcgat caggtcggta tcgccctgtc gcaggttgat cagggaattc ggcagtccaa
     4261 gccggtccgc gaggatttgc ggcatggtgg tttcgtggcc ttgaccgatg gtctgggtgc
     4321 cggtgatcag cgatacggaa ccgtcgcgct cgaagcggat atcgacattt tccgtgggta
     4381 agcctccggt ggccttgatg tggcaggcga gcccgaatcc ccgtcgcctg ccggccgctt
     4441 cactggcgcg gcgccgtgcc gggaagttgg ccatgtcggc attgccgatc gcgcgttcga
     4501 aatgggcgtg aaagtcgccg ctgtccacga cgaagttgaa agcgttcgtc atcggcagta
     4561 cagtaacgaa attgcggcgg cgaaggtcga ggcggtcgaa accgcattgc gtcgcggcgg
     4621 cgtcgatcag gcgttccatg atgttgaccg cctcaccgaa accgggcccg cgggtcacgc
     4681 cgatcggggt ggtgttggtc aggaccgccc ggacatgcag cgcgatcgcg ggcaggttg
//
LOCUS       DASWQN010001030         7978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2210702, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001030 DASWQN010000000
VERSION     DASWQN010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210702"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3106)
                     /locus_tag="VGC82_17540"
     CDS             complement(<1..3106)
                     /locus_tag="VGC82_17540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0425127.1"
                     /translation="MIAKFFIERPVLANVLAILFVLIGGVSLFTLPIAQLPDVVPPTV
                     QVTTRYPGASAIDVMNTVALPIEQQVNGVQGMLYMQSNSASDGSYVLTVTFAIGTDLN
                     FAQVLVQNRVASAMAALPQAVQSQGVNVQQKSTAILGIVTLTASDGQYASQYDSLYLS
                     NYAAINLQQEMARLPGVGNVTVFGAGQYAMRIWLDPQLMQARGLMTSDVTSALQQQSA
                     QVTAGQIGAPPATGQSFQYTLNVAGRLTDAAQFENVIVKSGADGAVTRIRDIGRVELG
                     AQTYGQAFTSNGKPAAGMAVFQAPGANALTVEKEVRAKMAELSRAFPPGMRYDMPFDT
                     TKFVSASVTEVYHTLIEAGVLVLVVILVFLQDIRATLVPATTVPVTIIAAFAAMAALG
                     FTINLSTLFAIVLAIGIVVDDAIVVVEGAAHYIDTGLSGHDAAIRAMNDLFAPIIGIT
                     LVLMSVFIPAAFLPGLTGRLYAQFALVIAATALISAINAATLKPTQCALWLRPAVPPA
                     QRNMFYRGFNAVYGRLEYHYAGLIAAMTRRSLAMAVLALLLMGGGIYGLTRLPTGFLP
                     LEDQGYFLVSVQLPDSASLSRTQEVMAKVQRAVSVVPGVQDVICISGISVLDNSASLS
                     NAGVAYVVLKDWGERTSADQGLLGMYQALSTAVAPIEEATTLVVPPPAIQGIGNAGGF
                     SMQVELRDGSSDFFKLQSLAQTIAADAHTQSGLARLNTTVRAGVPQMSVAVNRTKAEA
                     LHVSTGQVFQTLATYVGSSFVGQINKFGLTFQVYTQADAAARVTPADIEALPVRTGDG
                     TMVPLGTVVEITPTVGPALVQLYNLYPSATIIGSAAPGFSSGEALDLMEQISRNVLPA
                     GSASEWTAMSYQEKAVGNQMYVVFGLAILLVYLVLAGQYESWLAPISVVLAVPLALLG
                     PVLTLSALGVDNNLYTQIGLMLLIALGAKNAILIVEVAREQRLLHGASIAEAAVGAAR
                     ARLRPILMTSFAFILGVVPLVLATGAGANARKSIGISVSTGMLASTCLAVVFVPCFFV
                     VIQQF"
     gene            complement(3103..4239)
                     /locus_tag="VGC82_17545"
     CDS             complement(3103..4239)
                     /locus_tag="VGC82_17545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0425128.1"
                     /translation="MRTSVWRAAAIGLTLSIAGCKKSNQYAPPPPAKVSVARPVAQKI
                     TRYLEATGNTAAIASVDLIARVQGFVQAISFTDGAAVKAGDILFTIEPLPYQAKLQQA
                     QAAEASAQAQLVNAQAGFARQESLQRNAVASVQNLDDARAQRDSAQANLVQAQANTQL
                     AAINYSYTRVLAPFDGRMSAHLVSVGDLVGTSPTKLATIVQMQPINVTFSVSEQQVLR
                     IRGNAIREGRRGEAIQKIPVEIGLQGEPGYPHRGHMDYAAPMVDPSTGTLLARGVLDN
                     RDGALLPGYFARVRVPLDSDVNALLVPDAALGADQGGRYVLVVGADHVVAAKHVAAGP
                     LEGPLRVIESGLNADDLVVVDGLQRAVPGQTVEPAPVKTADVAQ"
     gene            4485..4652
                     /locus_tag="VGC82_17550"
     CDS             4485..4652
                     /locus_tag="VGC82_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425129.1"
                     /translation="MPDEADDKAKPVPEAAKPTQQISQDTERMEDEEKIIEGRHDVNY
                     PALLTKDVPGG"
     gene            4805..5692
                     /locus_tag="VGC82_17555"
     CDS             4805..5692
                     /locus_tag="VGC82_17555"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0425130.1"
                     /translation="MDTNLSTQPSAVGVVPIRLGHPFGAEIIDVDLRGISDSGFAAIH
                     QAWIDHQVLLFRNQTLDDHDLIAFSRRFGDLDHAPVQENGQRIANGMPEIYVVSNVIE
                     NGVAIGSLGAGEAVWHTDMSYLPSPPKASMLYALEVPPVGGDTSFCDMYAAFETLPGS
                     LRDRALGMRVKHDGTYNSGGYVRQGVTPTDDPRNAPGTLHPLVCTHPESGRRMLYLGR
                     RRMAWIEGLDLPESEALLDEIWAHATSPEISFTHRWRVGDLVLWDNRCTMHRRDPFDS
                     TTRRIMHRTQVKGSAPPAA"
     gene            5813..7174
                     /locus_tag="VGC82_17560"
     CDS             5813..7174
                     /locus_tag="VGC82_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEY0425131.1"
                     /translation="MTDYDAIVVGAGQAGPSLARRMAAEGQRVAIIERNLFGGTCVNT
                     GCTPTKAMVASAYAAHLARRAQEYGVTVGGVSIDMKQVKARKDKIVLASRNGVEASLR
                     KTENITVVQAHAKFTSAHEIAAGAETLRAPRIFLNVGARPAIPSIPGLDTVSHLTSST
                     ILDLDHVPDHLVVIGGSYVGLEFAQMFRRFGSAVTVIEASSRLIHHEDTEISDAVREI
                     LQAEGIQVRLGAKTIQVSPGIRVNVEDATGSASIEGSHLLLAAGRQPNTDDLGLDEAG
                     VAVDSRGYIQVDDALRTNVANIWAMGECNGRGAFTHTAYNDFEIVADNLLRDGHRGLS
                     DRITAYALYTDPPLGRAGMTEHEALAAGHKVLVGSLPMSSVSRAIEKGETLGLMKLVV
                     DADTQRILGAAFLGTGGDEVVHCVLDLMYAKAPYTILQRVVHIHPTVAEFIPSLAAAL
                     KPP"
     gene            complement(7187..>7978)
                     /locus_tag="VGC82_17565"
     CDS             complement(7187..>7978)
                     /locus_tag="VGC82_17565"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425132.1"
                     /translation="AAFLPPAAIALIAIYVRLKCPESPYWVRTQDRKNRLKERLARGQ
                     ILGAEDQAWFSKADKVGIRQLFMPDLIKNTLLSTFIASTSVIAFSTIGLWMPLFLKEQ
                     HGFSAAEYGNFYIWWGLAGVVGICFAGWFIDKLGRRLGFAFMLLEATVFMTWWIYATG
                     KTELMVLGLLWGWGLLGVWGPITTYTAEMYPTRIRGVGNGFSWTIAMFCGYVLWPFVS
                     VYLRQTTGSFQAAFLLIPVIMVIQAVIIWFCSPEYAGKELDEIAV"
BASE COUNT         1541 a         2606 c         2425 g         1406 t
ORIGIN      
        1 cgaattgttg gattacgacg aagaaacaag gaacaaagac aaccgcgagg caagtcgaag
       61 ccagcatacc cgtcgatacc gatattccaa tcgacttgcg cgcgttcgcc ccggcacccg
      121 tggccagcac cagcggaacg acccccagga tgaacgcgaa cgacgtcatc aggatcggcc
      181 gcaaccgggc ccttgccgcg cctacggcgg cttccgcgat ggaagcgcca tgcagcaaac
      241 gctgctcgcg cgccacctcg acgatcagga tcgcgttctt cgcgcccagc gcgatcagca
      301 gcatcaagcc gatctgggta taaagattat tgtccacacc cagtgcggac agcgtcagga
      361 ccggccccag caatgccagc ggcaccgcga gaaccaccga aatcggtgcc agccagcttt
      421 cgtattgccc ggcgagcacc aggtaaacca aaagtatcgc caggccgaag accacgtaca
      481 tctgattgcc gacggccttt tcctggtacg acatcgcagt ccattccgat gccgacccgg
      541 ccggcaagac gttgcgcgaa atctgttcca tcaagtccag cgcctctccg gaactgaacc
      601 ctggtgccgc cgacccgatg atagtggccg atggatacaa gttatagagc tgcaccaatg
      661 ccggtccgac cgtgggcgtg atctccacga cagtgcccag cggcaccatc gtgccgtcgc
      721 cggtgcgaac tggaagcgcc tcgatatccg cgggcgtcac ccgcgccgcg gcatccgcct
      781 gcgtatagac ctggaacgtc aggccgaact tgttgatctg gcccacgaaa ctggacccca
      841 cataggtcgc gagggtttgg aacacctgtc cggtcgagac atgcagcgcc tcggccttcg
      901 tcctgttcac cgccaccgac atttgcggca cccccgcccg gactgtcgtg ttcagccggg
      961 ccaggccgga ttgggtgtgc gcgtcggcgg cgatcgtctg ggccagggac tgcaacttga
     1021 agaaatcgct gctgccgtcc cgcaactcca cctgcatgga gaacccgccg gcattgccga
     1081 ttccctgaat ggcgggcggc ggaacgacca gggtcgttgc ttcctcgatg ggtgcgacgg
     1141 cggtgctcag cgcctggtac ataccgagca gcccctggtc ggcggaggtc cgctcgcccc
     1201 agtccttcag gacgacataa gcgacgccgg cattcgacag cgacgcgctg ttgtccagaa
     1261 ccgatatccc ggagatgcag atcacatcct gcacgccggg cacgacgctg accgcacgct
     1321 gcactttcgc catgacctcc tgcgtccggc tgagcgaggc gctgtccggc aattgcacgg
     1381 acaccaggaa atagccttgg tcttccagcg gcaggaagcc tgtcggcaac cgcgtcaggc
     1441 cgtagatgcc gccgcccatc agcagcagcg ccaggacggc cattgcaagg ctgcgccggg
     1501 tcattgccgc gatcaggccg gcatagtggt actccaatcg gccgtagacc gcgttgaaac
     1561 cgcgatagaa catgttgcgt tgggccggcg gcacggctgg ccgcaaccac aacgcgcact
     1621 gggtgggctt cagcgtcgcc gcgttgatcg cgctgatcag tgccgtcgcg gcgatgacca
     1681 gcgcgaattg ggcatatagc cgtcccgtaa ggcccggcag gaacgcggcc gggatgaaca
     1741 ccgacatcag caccagcgtg atgccgatga tcggcgcgaa caggtcgttc atcgccctga
     1801 ttgcggcgtc atgcccgctc aatccggtat cgatgtagtg cgccgcgcct tccaccacga
     1861 cgattgcgtc atcgacgacg atgccgatcg ccagcacgat ggcgaacagc gtggacaggt
     1921 tgatggtgaa tcccagcgcc gccatcgccg cgaaggccgc gatgatcgtc accggcaccg
     1981 tcgtcgccgg caccagcgtt gctctgatgt cctgcaagaa caccaggatg acgaccagca
     2041 ccaggacgcc cgcctcgatc agcgtgtgat agacttcggt caccgacgcg gaaacgaact
     2101 tcgtcgtatc gaacggcata tcgtatcgca tgcccggcgg aaatgcgcgc gacagctccg
     2161 ccattttcgc gcgaacttcc ttctccacgg tcaaggcatt ggccccaggc gcctggaata
     2221 ccgccatacc cgccgccggc ttgccgttcg aggtgaacgc ctggccgtag gtctgcgccc
     2281 caagttcaac gcggccgatg tcgcggatcc gcgtgaccgc gccgtccgcg ccggacttga
     2341 cgatgacatt ctcgaactgc gccgcatcgg tcagccgtcc ggcgacgttc agcgtgtatt
     2401 ggaagctctg ccccgtggcc ggcggtgcgc cgatctgccc ggcggttacc tgcgcgcttt
     2461 gctgctgcaa ggcggaggtg acgtcgctcg tcatcaggcc ccgtgcctgc atcagttgcg
     2521 gatcgagcca gattcgcatc gcgtactgcc ccgcaccgaa caccgtgacg ttgcccacgc
     2581 cgggaagccg agccatctcc tgctgcagat tgatcgcggc atagttgctg agatacaggc
     2641 tgtcatactg gctggcatac tggccgtcgg acgcggtcag cgtcacaata ccgaggatgg
     2701 cggtggattt ctgctggacg ttgaccccct ggctctgcac cgcctgcggc agggccgcca
     2761 tcgcgctggc aacccggttc tgcaccagca cctgggcgaa gttcagatcc gttccgatgg
     2821 cgaaggtgac ggtcagcaca taactgccgt cgctggcgct gttcgactgc atgtacaaca
     2881 tgccctggac gccattgacc tgctgttcga tcggcagtgc gacagtgttc atgacgtcga
     2941 tcgcactggc gccggggtag cgggtggtaa cctgcaccgt tggcggcacg acatccggaa
     3001 gctgggcgat cggcagggtg aacagcgaga ctccgccgat cagcacgaac aggatcgcca
     3061 gcacattcgc caggacggga cgctcgatga aaaacttcgc gatcattggg ctacatccgc
     3121 tgtctttaca ggtgcgggtt ccaccgtctg gcccggtacc gcgcgctgca agccatccac
     3181 caccaccaga tcgtcggcat tcaggccgct ctcgatcacc cgcaaaggtc cttcaagcgg
     3241 gccggccgcc acatgcttcg ccgccactac gtgatccgcg cccaccacca gcacataacg
     3301 gccgccttgg tccgccccca gcgccgcatc cggcaccagc agcgcattca catcgctgtc
     3361 caacggcacc cgtacgcggg caaagtatcc cggcagcaac gcgccatccc gattgtccag
     3421 cacgccgcgc gccagcagcg tcccggtcga gggatcgacc atcggcgcgg cgtagtccat
     3481 gtgtccacgg tgcggatagc ctggctcccc ttgcagaccg atctccactg ggatcttttg
     3541 gattgcttcg cctcgccgcc cctcccggat agcattgccg cggatgcgta gcacctgctg
     3601 ctcgctgaca ctgaacgtca cgttgatcgg ctgcatctgg acgatcgtcg ccagcttggt
     3661 gggcgaggtc ccgaccaggt cgccaacgga aacgaggtgc gccgacatcc gcccatcgaa
     3721 cggtgccaga accctcgtgt agctgtagtt gatcgcggca agctgcgtgt tggcctgcgc
     3781 ctgcaccagg tttgcctgcg cgctatcacg ttgcgcccgt gcgtcatcca ggttctgcac
     3841 cgaggcaacc gcattgcgct gcaacgattc ctggcgcgcg aatccagcct gggcattgac
     3901 cagttgcgcc tgagcggagg cctcggccgc ttgcgcctgc tgcagtttcg cctggtacgg
     3961 cagcggttcg atcgtaaaca ggatatcgcc ggccttcact gcggccccat cggtgaaact
     4021 gatcgcctgc acgaatccct gcacccgggc aatgagatct acggatgcaa tggcggccgt
     4081 attgccggtg gcttcaaggt agcgagtgat cttctgtgcc accggtctcg cgacgctgac
     4141 cttggccggc ggtggcggcg catactggtt ggacttcttg cacccggcga tcgagagcgt
     4201 caggccgatc gcggcggcgc gccaaaccga ggttcgcatt cgtttcactt ttgcccaatt
     4261 tttatttgac gcttgcgaac gttagcgggg ttcaacgtct ttcgaccaga cccagtattc
     4321 tgggttggta tcgctcccat gggaaatatt tgttgtgcag tgcgcaaaac gcgcattagc
     4381 ctatagcagc cacgggcatt tgttgatcga tgttggcggc tgaatggatc caggccggcg
     4441 ttcagcgact gtcccccaac ccgcaccctt gcatggagaa caagatgccc gacgaagccg
     4501 acgacaaagc aaagcccgtt cccgaagcag cgaaacccac ccagcagatc agccaggaca
     4561 cggagcggat ggaggatgaa gagaagatca tcgaaggccg gcacgatgtt aattatcccg
     4621 cccttctgac gaaggacgtt cccggcggct gaccgcgggc agcgttggcg acttgaccag
     4681 ccgcccgcca cgagatcagg cacgcggctg gccagggacg acgatcgtca ctagattagt
     4741 tctcgatagt tcccgcggcc acaactcctg cggccacggt cgcggtcttc cgaggacgat
     4801 gcacatggac accaatttat cgacgcagcc gtccgccgtc ggcgttgtcc cgatccgctt
     4861 gggccacccg ttcggggcgg aaataatcga tgtcgatttg cgcggtatct ccgattctgg
     4921 cttcgcggcc attcatcaag cgtggatcga ccatcaggtg ttgctttttc gcaaccagac
     4981 tttggacgat cacgacttga tcgccttctc gcggcggttc ggcgacttag atcatgcgcc
     5041 ggtgcaggag aacggtcagc gcatcgccaa cggcatgccg gaaatttatg tggtgtcgaa
     5101 cgtcatcgag aacggcgtgg cgatcggcag cctcggggcc ggagaagccg tatggcacac
     5161 cgacatgtca tacctgccct ccccgcccaa ggcgagcatg ctctacgcgc tcgaggttcc
     5221 gccggtcggc ggcgacacct cgttctgcga catgtacgcc gccttcgaaa ccttgcccgg
     5281 ttccttgcgc gaccgggcgc tcgggatgcg ggtgaagcac gacggaacct acaacagcgg
     5341 cggctatgtg cggcaaggcg tgacgccaac ggacgatccg cgcaatgcgc ccggcacgct
     5401 gcacccgttg gtttgcaccc atccggaaag cggacgccgc atgctctatc tcggacgccg
     5461 ccgcatggcg tggatcgagg gtctggatct gccggagtcg gaggcgcttc tggacgagat
     5521 ctgggcgcac gcgacgtcac ccgagatcag cttcacgcat cgctggcgcg tcggcgacct
     5581 cgtgctgtgg gacaaccgtt gcaccatgca ccgccgcgac ccgttcgact ccaccacgag
     5641 acgcatcatg caccggaccc aggtcaaagg ttcggcgccg cccgcggcgt gaacgcgtcc
     5701 cgggcataag catggcggcg gttcggcagg tgccggggct tggttcgttg actgctgtgc
     5761 gtcaggtaac ttggttcggg aacaggatgg gccaacgtaa aggagatcgg tgatgaccga
     5821 ctatgacgcc atcgtcgtcg gtgccggaca ggccggcccg tcactggctc gacgaatggc
     5881 agccgaagga cagcgtgtcg cgatcatcga acgtaacctg ttcggcggca cctgcgtcaa
     5941 taccggctgc acgccgacca aggccatggt cgccagcgcc tacgccgccc acctggcgag
     6001 gcgggcgcaa gaatacggcg taacggttgg cggcgtcagc atcgacatga agcaggtgaa
     6061 agcgcgcaag gacaagatcg tcctcgccag ccgcaacgga gtcgaggcgt cgttacggaa
     6121 gaccgagaac atcaccgtcg tccaggccca tgcgaaattt acctcggcac atgaaatcgc
     6181 tgccggcgcc gaaacgctgc gagcgccgcg catcttcctc aacgtcggcg cccggccggc
     6241 catcccgtcg atccccggtc tcgacacggt ttcgcacctg accagcagca cgatcctgga
     6301 cctcgatcat gtaccggacc atctggtggt gatcggcggc agctacgtag gcctggaatt
     6361 cgcccagatg tttcgccgct tcggcagcgc ggtcacggtg atcgaggcgt cgtcgcgctt
     6421 gatccaccat gaggacacgg aaatttcgga cgcggtgcgc gagatcctgc aagccgaggg
     6481 aatccaggtc cgactgggcg cgaagaccat acaagtcagt cctggtatcc gggtcaacgt
     6541 cgaggatgct acaggcagcg cctcgatcga agggtcgcac ctgctgctgg ccgcgggccg
     6601 gcagccaaac accgacgatc tggggttgga cgaagccggc gtcgcggtcg actcccgtgg
     6661 ctacatccag gtcgatgacg cgttgcgcac caatgtggcg aacatctggg cgatgggcga
     6721 gtgcaacggc cgcggcgcgt ttactcatac agcgtataac gatttcgaaa tcgtggccga
     6781 caacctgctg cgtgacggcc accgaggcct gagcgatcgt atcactgcct acgcgcttta
     6841 caccgacccg ccgctcgggc gcgccggtat gaccgaacac gaggccctgg cggcgggaca
     6901 caaggttttg gtcggcagcc tgccgatgtc gagcgtctcg cgcgccatcg aaaaaggtga
     6961 aacgctcggt ctgatgaaac tcgtcgtcga tgccgatacg cagcggatcc tcggtgcggc
     7021 gttcctcggc accggtgggg atgaagtcgt gcactgcgtc cttgacctta tgtatgccaa
     7081 agcgccctac acgatcctgc aacgggtggt gcatattcat cccaccgttg cagagttcat
     7141 ccccagcctg gcagccgcgc tgaaaccgcc gtgaccccgg cggcaatcag accgcgattt
     7201 cgtcgagttc cttgccggca tattccgggc tgcagaacca aatgatgact gcctggatga
     7261 ccatgatcac cgggatcagc aggaatgcag cctggaacga ccccgtggtc tgacgcaggt
     7321 aaaccgagac gaacggccaa agcacgtagc cgcaaaacat ggcgatggtc cacgagaagc
     7381 catttccgac gccgcggatc cgggtcggat acatttcggc cgtgtaggta gtgatcggcc
     7441 cccatacccc gagcagcccc catccccaca gcaggcccag gaccatcagt tcggtcttcc
     7501 cggtggcgta gatccaccac gtcatgaaga ccgtcgcctc cagcagcatg aacgcaaagc
     7561 ccaggcggcg gcccaatttg tcgatgaacc agccggcgaa gcagatgccg accacgccgg
     7621 ccaatcccca ccagatataa aagttgccat actcagccgc gctgaaacca tgctgttcct
     7681 tcaggaacag cggcatccac agcccgatcg tgctgaatgc aatgactgag gttgacgcaa
     7741 tgaatgtcga cagcaacgtg tttttgatca ggtcaggcat gaaaagctgc ctgatgccga
     7801 ccttgtccgc cttgctgaac caggcctgat cctccgcgcc gaggatctgg cctcgggcca
     7861 gccgttcctt caaccggttc ttgcgatcct gggtgcggac ccaatagggc gattccgggc
     7921 atttgagccg cacatagatc gcgatcagtg cgatcgcggc cggcggtagg aaggcggc
//
LOCUS       DASWQN010001031         3870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1022611, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001031 DASWQN010000000
VERSION     DASWQN010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022611"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..507)
                     /locus_tag="VGC82_17570"
     CDS             complement(67..507)
                     /locus_tag="VGC82_17570"
                     /inference="COORDINATES: protein motif:HMM:NF023520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HEY0425133.1"
                     /translation="MTRVFRATRLFRMICLGSLIAGLAGCGAPSGRTCAPGYGAPTAV
                     FTLYLGTAIPGRGDLTDKEWQSFLNDTVTANLPNGYTILDARGAWMNPITRRTIKEPT
                     KILVVALPDVPESLAAVNRIRTKYQLEFHQQLVGMITEQACSAF"
     gene            complement(540..1811)
                     /locus_tag="VGC82_17575"
     CDS             complement(540..1811)
                     /locus_tag="VGC82_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY0425134.1"
                     /translation="MNPDTLFSGVRILGAGVDIIGDLLVRDGMIADFGRAFGRPDGAT
                     VIEAEGAVLCPGLVDMRVAVGEPGFEYRETIASAAVAAAAGGITTLAALPDTQPAIDD
                     PALVQLLRTKGEETGSLTILPYGAVTRGCRGEELAELGLLREAGAVAFTDGSRAIGPA
                     RLMRLALSYAMGFGARIVQHPEEPSLSSSGAATESEQATRLGLPGIPAAAEAIMVARD
                     IRLVELTGGAVHFAHVSTGEALALIRQAKAAGLSVTCDTAPPYFDLNETSIGNFRTYA
                     KLSPPLRKEHDRRAVVAALADGTIDAIASDHQPRDADDKRLPFALASAGGAGLATLLA
                     VTLAQVHNGSILLPAAIDLLTARPAAILGVASGRIAKDAAADLCLFDPERIWKVEAGL
                     LPGKAQNTPFDGRALEGVVLGTWKRGKRVFG"
     gene            complement(1801..2739)
                     /locus_tag="VGC82_17580"
     CDS             complement(1801..2739)
                     /locus_tag="VGC82_17580"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY0425135.1"
                     /translation="MFYRQKHLLAIEGLEPPAISQLLDLADSYVLLNRSGKTQRDLLR
                     GRTLINLFFEDSTRTRTSFELAGKRLGADVINMSVSTSSVSKGETLIDTAATLNAMQC
                     DLLVVRHDQSGAPNLLAQIVDAAVINAGDGTHEHPTQALLDALTIKRRKGRIAGLIVA
                     ICGDIAHSRVARSNIHLLTTMGCQVRVVGPPTLIPTEVADLGVTVFHDMQAGLAGADV
                     VMMLRLQRERMSRGQVPSAREYFRFYGLDANKLAHAKPDAIVMHPGPMNRGVEIDSGV
                     ADDPIRSVIREQVEMGVAVRMAVLDMLSRGHRRNEP"
     gene            complement(2893..>3870)
                     /locus_tag="VGC82_17585"
     CDS             complement(2893..>3870)
                     /locus_tag="VGC82_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425136.1"
                     /translation="NHYYIHTMEASQHPEAAVASAERLPGMMPSAGHLEHMPAHIMQR
                     VGRYADAAQANRNGAAADLAYFAKTKPLDYYAMYSAHNYQFLAFATAMQGRRADTLDA
                     VRKSRAIISDDLLTAMPGADWYVAETYSAMVRFGMWQEMLAEPAPNPQLTGLIVGYRY
                     GRTNALAAMGRLEEARVELAELEKRAAAATPDDGAGLSPAPDVFAVAVLVAKARLAAA
                     EARSDEAIALLTEATTKEDALAYDEPSDWFFPTRHLLGAALLRAGRAQDAEAVYHEDL
                     RRHPENGWALYGLAQSLTAQRKDADAGKWRQQFDAAWKDADIALTASAF"
BASE COUNT          672 a         1208 c         1268 g          722 t
ORIGIN      
        1 cacgccgcgg attaagggcc tgtcgatgtt gaatccgtcc ggtttcgccc gccaagactt
       61 taccggttaa aatgcgctac acgcctgttc tgtgatcatg ccgacaagtt gctggtgaaa
      121 ttcgagttga tacttcgtgc ggatgcgatt gaccgcagcc aggctttcgg gaacatcggg
      181 cagcgcgacc accaggattt ttgtcggttc cttgatggtc ctgcgcgtga tcgggttcat
      241 ccaggcgccg cgcgcgtcca ggatcgtgta gccgttcggc aggttcgccg tgacggtgtc
      301 atttaggaat gattgccatt ctttgtccgt gagatcgcca cggccgggga tcgccgtgcc
      361 caggtacagt gtgaaaactg ccgttggtgc cccgtagcct ggggcgcagg tccggccgct
      421 gggcgcgccg cagccggcca ggccagcgat caggctgccg agacaaatca tcctgaaaag
      481 gcgggtcgcc ctgaaaacgc gggtcaccct gaaagggcgg gacgtcggct ttccgcgcgt
      541 caaccgaaca cccgcttgcc gcgtttccag gttcccagaa ccacaccttc cagcgcgcgc
      601 ccgtcgaatg gggtgttctg ggcctttccc ggcagcaaac cggcctcgac cttccatatc
      661 cgctcggggt cgaacaggca cagatcggca gctgcgtcct tggcgatgcg gccgctggcg
      721 acacctagaa tcgcagcggg ccttgcggtc agcaggtcga ttgccgcagg caaaagaatg
      781 ctgccgttgt gcacctgcgc cagggtcacc gccagcaacg ttgccaggcc ggcgcctccc
      841 gccgaggcaa gcgcgaacgg caagcgcttg tcgtcggcat cgcgcggctg atggtcggaa
      901 gcgatcgcgt cgatcgtgcc gtcagccaat gcggcgacga cggcgcggcg gtcatgctct
      961 ttgcgcaatg gtggcgacag cttcgcatag gtccgaaaat tgccgattga ggtctcgttc
     1021 aggtcgaaat acggtggcgc ggtatcgcag gtgacggaaa ggccagccgc cttagcctga
     1081 cggatcaacg ccaacgcctc cccggtcgag acatgggcaa aatgcaccgc gccgccggtc
     1141 agttccacca agcggatgtc gcgcgcgacc atgatcgctt cggcggcggc gggtatgcca
     1201 ggtaggccca ggcgcgtggc ctgctcgctt tccgtagccg ctccgctgct ggataacgac
     1261 ggttcttcag gatgttgcac gatgcgggcg ccgaatccca tggcatagga cagcgccagg
     1321 cgcatcagtc tggctggtcc gatagccctc gatccgtcgg tgaatgccac ggcaccagct
     1381 tcgcgcaaca gacccagttc cgccagttcc tcgccgcggc agccgcgcgt gacggcacca
     1441 tagggaagaa tggtcaggct gccggtttcc tcacccttgg tgcggagcaa ttgcaccagc
     1501 gccgggtcgt cgatcgccgg ctgcgtgtcg ggcagtgcgg ccagcgtcgt gatcccaccg
     1561 gctgccgccg caacggcggc ggacgcaatg gtttcgcgat attcgaatcc cggctcaccg
     1621 actgcgaccc gcatatcgac caagccaggg caaaggaccg caccttccgc ctcgatcacc
     1681 gtggcgccat ccggccgccc gaacgcccgg ccaaaatccg cgatcatgcc gtcgcgcacc
     1741 agcaggtcgc cgatgatatc gacaccggcg ccaaggatgc ggacaccgga gaacagcgta
     1801 tcagggttca ttccgacgat ggccccgcga gagcatatcc agcactgcca tgcggacggc
     1861 gacccccatc tccacctgtt ccctgatcac gctgcgaatc ggatcgtcgg cgacgccact
     1921 gtcgatttcg acgccccggt tcattggtcc aggatgcatg acgatggcgt ctggtttggc
     1981 atgtgccagc ttgttggcat ccagtccgta gaagcggaaa tattcgcggg cgctggggac
     2041 ctggccgcgc gacattcttt cacgctgcaa gcgcaacatc atcaccacat cggcgcccgc
     2101 caacccggct tgcatatcat gaaaaacggt gacacccagg tcggcaacct ccgtcggaat
     2161 cagcgtcggc ggtccaacca cgcggacctg gcatcccatc gtcgtcagca gatggatgtt
     2221 ggaacgagcg acgcgggagt gcgcgatgtc gccgcagatc gcgacgatca aaccggcgat
     2281 acggcccttg cggcgcttga tggtcagcgc gtccagcagg gcctgggtcg gatgctcatg
     2341 cgtgccgtcg cccgcattga tgaccgcggc atcgacgatc tgggctaaca ggtttggcgc
     2401 tccggactga tcgtgtcgca ccaccagcag gtcgcactgc atcgcgttca gcgtcgccgc
     2461 agtgtcgatc agcgtctcgc ccttgctgac cgatgacgtg gagacggaca tgttgatgac
     2521 gtcggcgccc aggcgcttgc cggccagttc gaagctggtg cgggtgcggg tgctgtcctc
     2581 gaagaacagg ttgatcaagg tgcgcccgcg cagcaggtcg cgttgggttt tgcccgatcg
     2641 gttgagcaga acgtagctat cggcgaggtc gagtaattgg ctgatcgccg gcggttccag
     2701 cccttcgatc gccaggagat gtttctgccg gtagaacaca gggtctcgtt gcagcaatgg
     2761 gcgcggggcg tcaaccgggg gtggggccgc aaggatggcg caggtactga aagcttgtcg
     2821 aggttggccg gcagatctgc gaaatcagga tcgtccgccg aatttccccg ctgctcaggc
     2881 gcgcgcgggg cgtcagaagg cagaggctgt tagcgcgatg tccgcatcct tccaggcggc
     2941 atcgaattgt tgccgccatt ttccggcgtc ggcgtctttg cgctgggcgg tcagcgactg
     3001 ggcgaggccg tacagggccc agccgttttc gggatggcgt cggagatcct catggtaaac
     3061 ggcttcggcg tcctgtgccc tgcctgcccg caacagggcc gcgcccagca gatgacgggt
     3121 cgggaagaac cagtccgacg gttcgtcata ggcaagtgcg tcctctttgg tggtcgcctc
     3181 ggtaaggagc gcgatggcct cgtcgcttct ggcttcggct gcggccagcc tggccttcgc
     3241 caccaggacc gccacggcga agacatcggg cgccgggctt aggccagcgc cgtcatcggg
     3301 cgtggcagcg gcggcgcgtt tttccagttc ggccagttct accctggctt cttcgagacg
     3361 ccccatggct gccagggcgt tagtccggcc gtagcgatag ccgacgatca agccagtcag
     3421 ttgagggttg ggcgcaggtt ctgccagcat ctcctgccac atgccgaacc ggaccatcgc
     3481 agaataggtt tcggcgacgt accagtccgc gccgggcatc gcggtcagca gatcgtccga
     3541 aatgatcgcg cgagacttgc gaacggcgtc cagcgtgtct gcccgacggc cttgcatggc
     3601 tgttgcgaac gccaggaact ggtagttatg cgcgctgtac atcgcatagt agtcgagcgg
     3661 cttggttttc gcaaagtagg cgaggtcagc cgcggcaccg ttgcgattgg cttgggctgc
     3721 atcggcatac cggccgacgc gttgcatgat atgcgcgggc atatgctcca ggtgaccggc
     3781 ggagggcatc atgcccggca gacgttcggc tgaagcgacc gcagcctcgg ggtgctgcga
     3841 tgcctccatg gtatggatgt aataatggtt
//
LOCUS       DASWQN010001032         5477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_891309, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001032 DASWQN010000000
VERSION     DASWQN010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891309"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /gene="pgk"
                     /locus_tag="VGC82_17590"
     CDS             complement(<1..551)
                     /gene="pgk"
                     /locus_tag="VGC82_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY0425137.1"
                     /translation="MSFRSLDNVDVAGKRVLLRADLNVPVRDGKISDLTRIERLSPTI
                     RELSGKGAKVIVCSHFDRPKGKRVPEMSLAPMAAALGEVLGQRVRFVEDCTGTAAEQA
                     VDLLSRGGVLVLENTRFHAGEESNDPAFAAALAKLADIFVNDAFSAAHRAHASTEGVA
                     HLLPSYAGRLMQAELEALDAALG"
     gene            complement(776..1789)
                     /gene="gap"
                     /locus_tag="VGC82_17595"
     CDS             complement(776..1789)
                     /gene="gap"
                     /locus_tag="VGC82_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY0425138.1"
                     /translation="MAVKVAINGFGRIGRLVLRAIVESGRDDVVPVAINDLGSVEANA
                     HLFAYDSVHGRFPGQIEVSGDTITIRHNGKVYGPLKVSAERDPTKVPYQGVDVALECT
                     GIFASKSKASALLTAGARKVLISAPADEVDATIVYGVNHKTITKDMTVISNASCTTNC
                     LAPMAKVLHERFGILRGYMVTIHAYTGDQNTVDTLHKDLHRARAAAVSAIPTSTGAAK
                     AVGLVLPELKGKLDGTAIRIPTPNVSLVSLDVNLSKQATKEEINAAMQDAAKGELAGI
                     MDYNKAPLVSIDFNHNPASCTFDATQTAVVDGALARVMGWYDNEWGFSNRMSDTAALF
                     GSL"
     gene            complement(1912..3876)
                     /gene="tkt"
                     /locus_tag="VGC82_17600"
     CDS             complement(1912..3876)
                     /gene="tkt"
                     /locus_tag="VGC82_17600"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY0425139.1"
                     /translation="MTPLRRMADAIRALAIDATEQSKSGHPGLPMGMADVATALWTRF
                     LKFDAADPRWPDRDRFVLSAGHGSMLLYALIYLTGYEGLSIDDIRNFRQLHSPAAGHP
                     EYGEHPAIETTTGPLGQGLATSVGMAIAERNMAARFGKSLVDHRTWVIASDGDLMEGI
                     SHEAAALAGHLGLSKLTVMYDDNHVSIDGDTALSYSDDVLKRFSAYGWATKRVDGHDP
                     SQINAALSFAVRSKKPTLIACRTIIGLGAPTKAGTNAVHGSPLGPKEAAGAKELLGWH
                     EPPFETPADLMERWHAVGGRGAAARRSWLKRLARHNQRGEFERVMTGRLPDNWHEAVV
                     ELKQQIADDRPKLASRASSQKCLETLVPATPELIGGSADLTGSNLTLVKGMASISAGN
                     YGGRYIHYGIREHGMAAAANGMALHGGIIPYVATFFVFSDYLRSALRMGAIMRQRVIY
                     VLTHDSIGLGEDGPTHQPIEHLASLRAMPNLHVFRPGDTMETAECWELAIRRADGPSL
                     LVLSRQALPAWRTDTSENRCARGGYVLAEAEGPRRATIIATGSEVPIAMAAREKLAGD
                     NIAVAVVSLPCWELFAQQDDGYKAQVLGGAPRVGIEAAGSFGWEQWLGPDGIFIGMTG
                     FGASANYEDLYNYFGITPEAVVAAVMRRMG"
     gene            complement(4130..5281)
                     /locus_tag="VGC82_17605"
     CDS             complement(4130..5281)
                     /locus_tag="VGC82_17605"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425140.1"
                     /translation="MSPALPRFLQLYAALFLAFGVASPFFGAFLNERGLPPASIGLVL
                     AAGTAVRLVAGPAGGRLADRLGAPRLVLVAYAAAASCVAFGYLPAQGLWAVLLVALAH
                     AATLAPLTPIADALTLRAGSSGSGKHFEYGWVRGAGSASFIVGTLVAGQAVGRFGLSA
                     IIWLNATLLGITAWCAWMVPDLLSRTAACERDRAGGVASLLRLPAFRRLMLVAALIQG
                     SHVLHDGFVVIYWRSVGIGDAAIGLLWSESVAAEVLVFLLFGGWLLDRLSPATASILA
                     ALGGIVRWAVLAETSSAAASALVEPLHGLTFALQHLACMRLIAAVVPPGLAATAQAFY
                     GTVAVGLATALLMLGSGPLYMRFGAGGFWIMAGLCAVAIPIARGLPRQA"
     gene            complement(5287..>5477)
                     /locus_tag="VGC82_17610"
     CDS             complement(5287..>5477)
                     /locus_tag="VGC82_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425141.1"
                     /translation="RHGVGIGKKMFSGSEGHGTISNAALHVRLRQAEINGAGSRRNPE
                     DRPMRRDPAAMGAPERMI"
BASE COUNT         1029 a         1877 c         1669 g          902 t
ORIGIN      
        1 tttcccagcg ccgcatccag cgcctccagt tctgcttgca tcaaccgccc ggcgtaggag
       61 ggaagcaggt gggcaacacc ctcagtgctg gcatgggcgc gatgagcggc cgagaacgcg
      121 tcgttgacga agatgtcggc aagcttcgcc aatgcggcgg cgaacgccgg atcgttgctt
      181 tcctcaccgg catggaagcg ggtgttctcc agcaccagga cgccgcctcg ggacagcaga
      241 tccaccgcct gttcggccgc ggtaccggtg caatcttcca cgaagcggac gcgctgaccc
      301 agaacctcgc ccagtgccgc ggccatcggg gcaagcgaca tctcggggac acgcttgcct
      361 ttggggcggt cgaaatggct gcagacgatg accttggcgc cttttcccga caactcccgg
      421 atggttgggg atagacgctc gatccgggtc aggtcgctga tcttgccatc gcgcaccggg
      481 acattgaggt cggcacgcag caggacgcgc ttcccggcga cgtccacatt gtcgaggctt
      541 cggaaagaca tgcaggcact ccagttaaag acagagatat ccgcagagcc tgtcttcccg
      601 ctgatcgcgc cgccgatctg gcgtcgtggc cgtccatacc gagcgggcct gaccgaacac
      661 agcaggccga accgaaccga gcgggcctga cctagctggc ctgaccgagc cggcccgacc
      721 gagccggctg accggccgtg gcagtcgcca ctgcactgca ccggccatgc gttggttaca
      781 agctaccgaa aagcgccgcc gtgtcggaca tccggttgga aaagccccac tcgttgtcgt
      841 accagcccat gacccgtgcc agcgcaccat ccacgaccgc cgtctgggtc gcgtcgaacg
      901 tgcaggatgc cgggttgtgg ttgaagtcga tgctgaccag gggcgccttg ttgtagtcca
      961 tgatgccggc cagctcgcct ttggcggcat cctgcatcgc tgcgttgatc tcttccttcg
     1021 tcgcctgttt agagaggttc acgtcaagcg acacgagcga cacattcggc gtcgggatgc
     1081 gaatagcggt gccgtccagc ttgcccttca actccggcag caccagtccg acggccttgg
     1141 ccgcaccggt ggaggtcgga attgcggaaa ccgccgccgc tcgggcgcga tgcaggtcct
     1201 tgtgcagcgt gtccacggtg ttctggtcgc cggtgtaggc gtggatcgtc accatatagc
     1261 cacgcaggat gccaaaccgt tcgtgcagca ccttcgccat cggcgccaga cagttggttg
     1321 tgcaggatgc gttcgagatc accgtcatgt ccttggtgat cgtcttgtgg ttcacgccgt
     1381 aaacgatcgt cgcatccacc tcgtccgctg gggccgagat cagcaccttg cgcgcaccgg
     1441 ctgtcagcaa tgcagacgcc ttcgacttgc tggcgaagat acctgtgcat tccaacgcca
     1501 cgtccacacc ctggtagggc accttggtcg gatcacgttc ggccgacacc ttcagcgggc
     1561 cataaacctt gccgttgtgg cgaatggtga tggtgtcgcc ggacacctcg atctggccgg
     1621 ggaaccggcc atgcacgctg tcataggcaa acagatgcgc gttcgcttcc acgctgccca
     1681 gatcgttgat ggccaccggc acgacatcgt cccggccgct ttcgacgata gcgcgcaaca
     1741 cgaggcgccc gatccgtcca aacccattta tcgccacttt aacggccatg acctgctccc
     1801 tttccgctct tcccgatacc gattcttacc cggcatcacc ggctaacgcc acactcgttg
     1861 aaggaaatca acggcagcgc cttgcacaaa tccgctgtcc cgacgcgtgc tttaccccat
     1921 ccggcgcatg acggccgcga cgaccgcctc gggcgtgatg ccgaagtagt tatacaaatc
     1981 ctcgtaattc gccgatgcgc cgaagcccgt catgccgata aagatgccgt ccggccccag
     2041 ccattgctcc cagccaaagc tgccagccgc ctcgatcccg acccgcggcg cgccacccag
     2101 cacctgggct ttatagccat cgtcctgctg cgcgaacaac tcccagcacg gcaaggatac
     2161 gactgcgacg gcgatgttgt cgccggcgag cttctcccga gcggccatgg cgatcggcac
     2221 ctccgatccg gtggcgatga tcgtggcccg gcgggggcct tccgcctccg ccagaacata
     2281 tccgccacgc gcgcagcggt tttcgctggt atccgtccgc catgccggca gcgcctggcg
     2341 cgacaacacc agcaagctgg ggccgtcagc acgccgtatc gcgagttccc agcactccgc
     2401 ggtttccatc gtgtcgccgg gcctgaagac gtgcaggttg ggcatcgcgc ggaggctggc
     2461 gaggtgctcg atcggctggt gcgtcggccc gtcctcaccc aggccaatcg agtcgtgcgt
     2521 cagcacatag atgacccgct ggcgcatgat cgcgcccatg cgcaaggccg agcgcaaata
     2581 gtcgctgaac acgaagaacg tcgcgacata gggaatgatg ccgccgtgta gtgccatgcc
     2641 gttcgccgcg gcggccatgc cgtgctcgcg aatgccgtaa tggatatagc ggccgccgta
     2701 gttgccagcc gagatcgacg ccatcccctt taccaacgtc agattcgacc cggtcagatc
     2761 ggccgacccg ccgatcagct ccggcgtcgc gggcaccagg gtctccaggc atttttggct
     2821 ggatgcccgg ctcgccagct ttggcctgtc gtcggcgatc tgctgcttca gctcgaccac
     2881 cgcctcgtgc cagttgtccg gcagccggcc ggtcatcaca cgttcgaact cgccgcgctg
     2941 attgtgccgg gcgaggcgtt tgagccagga acggcgtgcg gctgcccccc ggccgccgac
     3001 ggcatgccag cgctccatca agtcagccgg tgtttcgaag ggcggttcgt gccaaccgag
     3061 cagttccttg gccccggcgg cttcctttgg accaagcggc gagccatgca cggcgttggt
     3121 acccgctttg gtgggcgcgc ccaggccgat gatggtgcgg caggcgatca gggtcggctt
     3181 cttcgaacgc acggcgaagg acagtgccgc attgatctgg gacggatcgt gcccatcgac
     3241 ccgcttggtg gcccagccgt acgcggaaaa ccgcttcaga acatcgtccg aataagacag
     3301 agccgtgtcg ccgtcgatcg agacgtggtt gtcgtcatac atgaccgtca gcttcgacaa
     3361 ccccagatgg ccggccaatg ctgccgcctc gtggctgatc ccctccatca agtcgccatc
     3421 cgacgcgatc acccaggtgc ggtgatcgac cagcgacttg ccaaatcgcg cggccatgtt
     3481 gcgttcggcg atcgccatgc cgacggaggt cgccaaaccc tggcccaacg ggcctgtcgt
     3541 ggtctcgatg gccggatgtt caccgtattc gggatgcccg gcggccggcg agtgcaactg
     3601 gcgaaaattg cggatgtcgt cgatcgacag accctcgtat ccggtcaggt agatcaatgc
     3661 gtacagcaac atcgaaccat ggccggcgga cagcacgaag cgatcccggt caggccagcg
     3721 cggatcggcg gcgtcgaact tcaggaagcg cgtccatagc gcggtggcaa catcggccat
     3781 ccccatcggc aatccagggt gcccggattt cgactgctcc gtcgcgtcga tcgccaaggc
     3841 gcggatcgca tcggccatgc ggcgcaacgg agtcattggg cgcgccagaa tctccgcctg
     3901 gagggcgagt gcgggattat cggatgcagg tttgtcgtgc gacagctcgg cgacggcagc
     3961 catgcgaact ccttgaagtg ccgtttaatc ggcggtggac atcgcggcaa ccccggtgaa
     4021 ccggaaggcc gaccgccggg acggtcaaac aggaacggca ccacgcgggc tcggttgcgc
     4081 ggggccgttt ccaacatgga tggtgagatc ccaaggctat atagcggagt cacgcttgac
     4141 gcggcaggcc ccgggcgatc gggatcgcaa cggcgcaaag tcctgccatg atccaaaagc
     4201 cgccggcccc gaaccgcata tacaacggcc ccgaccccag catcagcagc gccgtcgcca
     4261 ggccgacagc gacggttcca taaaatgcct gggccgtcgc cgccaagccg ggcggcacaa
     4321 ccgccgcgat cagccgcata caggccaaat gctgcagcgc gaacgtcaat ccgtgcaacg
     4381 gctcgaccaa tgcagacgct gccgccgaac tcgtctccgc cagcaccgcc cagcgcacga
     4441 tgccgccaag ggccgccagg atcgaagccg tcgccgggct tagacggtca agcaaccacc
     4501 cgccgaacag caggaaaacc aaaacttcgg ccgcgacgga ctccgaccac agcagaccaa
     4561 tggcagcatc gccgatcccg acgctgcgcc agtagatgac gacgaaccca tcgtgcagga
     4621 catggctgcc ctggatcaac gccgccacca gcatcagccg gcggaacgcc ggcaaacgaa
     4681 gaagcgatgc caccccgccc gcacgatccc gctcgcacgc agcggttcgg ctgagcaggt
     4741 ccggcaccat ccaagcgcac caggccgtga tcccgagcag cgttgcgttc aaccagatga
     4801 tggcactcaa cccaaacctg ccgacagctt ggccagccac cagcgtgccg acgataaacg
     4861 aggccgaccc ggcgcctctg acccagccat actcgaagtg cttaccggat cctgagctcc
     4921 cggcccgaag agtcagggca tcggcgatcg gtgtcagcgg agccagcgtc gccgcgtgtg
     4981 ccaaggccac cagcaggacc gcccacagtc cctgtgccgg caggtagccg aacgccacgc
     5041 aagacgccgc cgctgcatag gctaccagca cgagtcgagg cgcccctagc cgatccgcga
     5101 gcctaccgcc cgcgggaccc gccaccagac gcacggcggt tccggctgcc aacaccagtc
     5161 cgatggaagc cggcggcaag ccccgctcgt tcaggaacgc accgaagaac ggcgaggcca
     5221 ccccgaacgc caggaacagc gcggcgtaga gctgcagaaa acgcggcagg gcaggggaca
     5281 tcctggttaa atcattcttt ccggcgcccc cattgccgcc gggtctcggc gcatcggacg
     5341 atcctcgggg ttccgccgtg aaccggctcc attgatttcg gcctgcctaa gcctgacatg
     5401 caacgcagca ttggagattg tcccatggcc ttcgctgccg ctgaacattt ttttgccaat
     5461 accaacccca tggcgcg
//
LOCUS       DASWQN010001033         9619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1549983, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001033 DASWQN010000000
VERSION     DASWQN010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 9619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9619
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549983"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VGC82_17615"
     CDS             complement(<1..222)
                     /locus_tag="VGC82_17615"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0425142.1"
                     /translation="MSEQPISPREAVRATVPKMIEVADNVIYGDIWERPGLSKRDRSL
                     VVLATLIGTYRPEQLRGHLGRVLDNGVTRA"
     gene            complement(460..885)
                     /locus_tag="VGC82_17620"
     CDS             complement(460..885)
                     /locus_tag="VGC82_17620"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0425143.1"
                     /translation="MPAQSLNHYTIKARDLERTKAFYEEIVGLKTGDRPPLNFPGYWL
                     YCGDVPTVHLIGHRAEDAAISDGPSEPAATGRLDHIAFACNGLKDMRSNLQAHDIKFD
                     ERVLPRLNMTQLFYLDPDGIAVECNFDASETETSAAKGY"
     gene            complement(900..2033)
                     /locus_tag="VGC82_17625"
     CDS             complement(900..2033)
                     /locus_tag="VGC82_17625"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0425144.1"
                     /translation="MTEIHPQVTRALGLHPLIRQEGNEFNQRRELTPPVVKALKEGDF
                     FRMLQPRSIGGMELKPSDFARVTEALAAADGSVGWVVCQGNGCSMSAAYLDPSVAREI
                     FGPIDGILAWGPPGAPFEAEPVEGGYCISGTWRFASGSQNATFLGAHMKVKGRKEVRT
                     MLFPKSSVTMVDIWHTLGLRGTASNEYVVKDLFVPADHTMLRDQAVDRREEGPLYRFG
                     SSQLYSAGFAGVGLGLARGLIDAFLDLPATKISRGASKPLRENNVVQSQLAQSEAKWH
                     SARAFLHNTLDEIYDYVIRHGEMTERHAAMMRLASTWAIQQSREAVNTMFHCAGSVAV
                     FEEQPFERRLRDIHTVSQQSQGRQLHYESVGQIMLGLPPENVF"
     gene            complement(2114..3502)
                     /locus_tag="VGC82_17630"
     CDS             complement(2114..3502)
                     /locus_tag="VGC82_17630"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425145.1"
                     /translation="MFDRLDRATSLTSNQIKIIAAAIIGDMLEFFDYYLIGFVLAFII
                     KPWHLTFGQSAIILLSSGVGAILGSVIWGRLADVYGRRKVFIGTVINFSLASGVLAFT
                     PDNGWWFLSIFRFFVGFGVGGLYCVDLPLVQEFVPARMRGFIGGLVTVFIPLGVMIAS
                     SFAAFTTDTIGWRGLFLVGVVPAAFTLLVRAWVPESPHWLAGQGRTEEARKSLAWALM
                     VTPAELPLPAKSMKQDTAPWSDLLLYPKSLAVSFMASIGAQTASYGIGLWAPTLFVLQ
                     LGVTPAYAAYLFLYVKLAGIAGRISFAWLSDAIGRRNAGMLAGFGGAITIAAAGLLHE
                     TFINGVSLFWLSIVIADFFYDGGFAVIGPYLAEVWPTRLRTTGMGASYGFGGIGKIIG
                     PLGLALIVGSADVITPKATMDAIGPSFAYFTGWMVLCGCAFLFFGFETGNKSIASVDR
                     ELENKAPAPIGE"
     gene            complement(4149..5594)
                     /locus_tag="VGC82_17635"
     CDS             complement(4149..5594)
                     /locus_tag="VGC82_17635"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0425146.1"
                     /translation="MLAPDRPRQAAVFDHLRHSILTGALPGGGRLPPSRSLANELGVA
                     RQTVVLAYERLAAEGYVRGRTGSGTYITTDLPDQAPAPVATPSSAAVALSARGTHLAG
                     ISATATARDPVLGLLLAGGLPAPDLFPIKAWARCASRVLKPLSGELSSYPPPQGLLML
                     REQIAAHLAATRGLVADPGCIVVTGGTQQALRTAADLLLDPDDPVWVEDPGYIAGRGA
                     LLAAGARIVPIPSDAEGIDVAAGVRIAPDARMALVAPSHATPLGGALPIGRRLALLDW
                     AGRAKAWVLEDDCDSEFRWEGKPLPPLATLDKAGQVIYCGTFSKTLAPALRMGFAIVP
                     APLVPAFVRLRTLTDRGTDAFTQATLAEFMRLGLLSPHIRKMRTEYSRRRDALLEAMK
                     RLVRHATPLAAPGGLHMVARLPDDVDEATAVRACRSRDLAVSPLRAYYPGKPRMTGLV
                     VGFAATPTSMANEVARRFEAALTSVGRSRSA"
     gene            complement(5728..5931)
                     /gene="rpsU"
                     /locus_tag="VGC82_17640"
     CDS             complement(5728..5931)
                     /gene="rpsU"
                     /locus_tag="VGC82_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY0425147.1"
                     /translation="MQVLVRDNNVDQALKALKKKMQREGIFREMKLRRHYEKPSERRA
                     REAAEAVRRARKLERKRLEREGF"
     gene            6074..6607
                     /gene="def"
                     /locus_tag="VGC82_17645"
     CDS             6074..6607
                     /gene="def"
                     /locus_tag="VGC82_17645"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY0425148.1"
                     /translation="MAILKIARMGHPVLLQKATPVADPGAPEIRRLVDDMMETMMDAP
                     GVGLAAPQVYQPLRLFVFRVPEGRETRDPDDITLANTVLINPTLELIGEERALGWEGC
                     LSIPGLRAAVPRARRIRYRGVDCDGNVTEREATGFHARIVQHEYDHLDGILYTMRMSD
                     FRHFGFTDELDRAGTQG"
     gene            6604..7287
                     /locus_tag="VGC82_17650"
     CDS             6604..7287
                     /locus_tag="VGC82_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HEY0425149.1"
                     /translation="MIRTPERMPERDAAILAMLPHVAFDGWTKRALRAGVADAGMPAD
                     EAELLFPLGTVDMIETFCDLADRRMEAASAELAETRLSARVRAVIALRLRQNRPFKDA
                     IRRALAILALPQNARAAAFCSARTADAIWHAAGDRSADFSWYTKRAIMTAVFGATVLF
                     WLRDGSEDDADTLAFLDRRLAAIGRMGRLRGRADRLVARLPRLPLPRLPRVRMPPCQP
                     AATGVGDTG"
     gene            7352..9157
                     /gene="lepA"
                     /locus_tag="VGC82_17655"
     CDS             7352..9157
                     /gene="lepA"
                     /locus_tag="VGC82_17655"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY0425150.1"
                     /translation="MTDTPIERIRNFSIIAHIDHGKSTLADRLIQVTGGLTAREMTAQ
                     VLDNMDLEKERGITIKAQTVRLSYTAKDGLTYELNLMDTPGHVDFAYEVSRSLAACEG
                     SLLVVDASQGVEAQTLANVYQAIEANHEIVPIINKIDLPAAETERVKQQIEDVIGLDT
                     SHAVEISAKTGLNIEGVLEALVTRLPAPKGDGKAPLKALLVDSWYDQYLGVVILVRIK
                     DGRMKRGQRIRMMSSGAVHSVEQVGVFTPKMIPTDDLGPGEIGFITAAIKTVADCNVG
                     DTITDDRMPATAALPGFKPSIPVVWCGLYPIDADDFEKLRDSLGKLRLNDASFHYDPE
                     TSAALGFGFRCGFLGLLHLEIIQERLTREFDLELIATAPSVVYHVYKTNGQMQELHNP
                     VDMPDGSVIEHIEEPWIKATIMVPDEYLGSVLGLCTERRGQQVDLTYVGNRAMAVYRI
                     PLNEVVFDFYDRLKSVSRGYASFDYAMDGYAESDLVRISILVNGDLVDALSFIAHRAA
                     AENRGRQICARLKDLIPKQLFKIAIQAAIGSRVVARETISALSKDVTAKCYGGDISRK
                     RKLLEKQKEGKKRMRQFGKVEIPQSAFLAALKMDA"
     gene            9294..9383
                     /locus_tag="VGC82_17660"
     tRNA            9294..9383
                     /locus_tag="VGC82_17660"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9328..9330,aa:Ser,seq:gga)
BASE COUNT         1771 a         2984 c         3143 g         1721 t
ORIGIN      
        1 ggctcttgtc acaccgttgt ccaagacgcg tccaaggtgg ccgcgaagct gctccggccg
       61 gtaggtgccg atcaaggtcg ccaagacaac gaggctccga tcgcgcttac ttaaacctgg
      121 ccgctcccag atatcgccgt agataacatt gtcggccacc tcgatcatct tggggacggt
      181 tgcccgcacg gcttcgcgcg gactgatcgg ttgttcggac atcgaatttc tccggataag
      241 gttcgaacca ggacgccgct ccggatgtca tggtgtccgc caaggatccg gttcgatcaa
      301 ctgctccaat tgatgcatga tccgactacc ccgtcttgat aggcgaaggc ccaccattca
      361 cgccggtgca gattgagcac cgcaagccgt ggtgaagggc cggcccacca agacggatct
      421 actggcgggc gctcgaccgc gcttatgtgc ggacggacat caatatcctt tagcggccga
      481 tgtctcggtt tcggacgcgt cgaagttgca ttccaccgcg attccgtccg ggtccaggta
      541 gaataactgc gtcatgttca gccgcggcag gacccgctcg tcgaacttga tgtcgtgggc
      601 ctgcaggttg gaccgcatgt ccttcaagcc attgcaggca aacgcgatat ggtccagccg
      661 gccggtcgcc gcgggttcgg aggggccgtc gctgatggcg gcatcctccg cgcggtgacc
      721 aatcaggtgc accgtgggga catcgccgca gtacagccag tagccgggga agttcaacgg
      781 cggccgatca cccgtcttca ggcccacgat ttcctcatag aacgccttgg tgcgctccag
      841 gtcgcgggct ttgatcgtgt agtggttcaa cgactgtgcc ggcatcttcg gttctcctgt
      901 cagaatacgt tttcgggagg caggccgagc atgatctgcc cgaccgattc gtagtggagc
      961 tgacggccct gggactgttg cgacacggtg tgaatgtcgc gaagccgcct ttcaaacggc
     1021 tgttcctcga acaccgcgac cgatccggcg cagtggaaca tcgtgttcac ggcctcccgc
     1081 gactgctgga tcgcccaggt ggacgccaga cgcatcatcg ccgcgtgacg ctcggtcatt
     1141 tcaccgtgcc ggataacgta gtcatagatt tcatcgaggg tgttgtgcag gaacgcgcga
     1201 gcagagtgcc atttcgcctc ggactgcgcc agttgcgact gcacgacgtt attctcgcgc
     1261 agcggcttgg aggcgccgcg cgatatcttc gtcgccggca gatccaggaa agcatcgatc
     1321 aggccgcgcg ccaggccgag gccaaccccg gcgaaccctg ccgagtaaag ctggctgctg
     1381 ccgaaccggt acagcggtcc ctcctcgcgc cggtccaccg cctggtcgcg cagcatcgtg
     1441 tggtcggccg gaacgaacag atccttgacg acatactcgt tgctggcggt gccgcgcagg
     1501 ccaagcgtgt gccagatgtc caccatcgtc acgctggact tcgggaacag catcgtgcgg
     1561 acttccttgc ggcccttcac cttcatatgg gcgccaagga aagtagcgtt ctggctgccg
     1621 ctggcgaacc gccaggtacc ggagatgcag tatccgcctt cgacgggctc agcctcgaac
     1681 ggggcgccgg gggggcccca tgcaagtata ccatcgatcg ggccgaagat ctcccgcgcc
     1741 acgcttggat cgaggtaggc cgcagacatc gagcatccgt tgccctggca caccacccag
     1801 ccgacggagc catcggccgc ggccagcgcc tcggtgactc gggcgaagtc ggacggcttc
     1861 aattccatgc cgccgatcga gcgcggttgc agcatacgaa agaaatcgcc ttctttcagt
     1921 gcttttacga ccggcggagt cagctcgcgg cgctgattga actcgttgcc ttcctgccgg
     1981 atcaacggat gcagcccaag ggcgcgggtg acctgggggt ggatttcagt catccgaatg
     2041 cctccgcatc aaaggcgaga tcgccgttca catgaaggat gacgcatttt catccgattg
     2101 aaaagcaagg ctgttactct ccgatcgggg ctggggcttt gttttccagt tcgcgatcaa
     2161 ccgaggcgat cgatttgttg ccggtctcga agccgaagaa cagaaacgcg cagccgcaca
     2221 ggaccatcca gccggtgaag taagcgaagg agggaccgat cgcgtccatg gtggctttgg
     2281 gcgtgatgac gtcggccgaa ccgacgatca gcgccaggcc aagcggcccg atgatcttgc
     2341 cgatgccgcc gaacccatag gaagcgccca tgccggtggt gcgcaggcgg gtcggccaga
     2401 cctccgcgag gtaggggcca atgaccgcga acccgccgtc atagaagaag tcggcgatga
     2461 cgatggaaag ccagaacagc gagacgccgt tgatgaacgt ttcatgcagc aggccggcgg
     2521 cggcgatcgt gatggctccg ccgaagcctg ccagcatgcc ggcgttacgg cggccgatcg
     2581 cgtccgacag ccaggcgaag ctgatgcgcc cggcgatgcc cgcaagcttc acgtacagaa
     2641 acaggtaggc ggcgtaggct ggcgtcacgc ccagttgcag cacgaacaag gtcggcgccc
     2701 agaggccgat accgtaggat gcggtctgtg cgcctatact ggccatgaaa gatacggcga
     2761 ggcttttggg gtatagcagc aggtcgctcc atggcgccgt gtcctgcttc atggatttcg
     2821 ccggcagcgg cagttcggcc ggcgtcacca tcaaggccca cgccagggat ttccgtgctt
     2881 cctcggtgcg gccttgaccg gccagccagt gcggggactc cggcacccag gcgcgaacca
     2941 gcaaggtaaa ggccgcgggg acgacgccga ccaggaacag gccgcgccag ccgatggtgt
     3001 cggtcgtgaa cgcggcgaag gacgatgcga tcatgacgcc gagcgggatg aagacggtga
     3061 ccaggccgcc aatgaagccg cgcatgcgag cgggcacgaa ctcctgcacc agcggcaggt
     3121 caacgcagta caggccgccc acgccaaacc cgacaaagaa gcgaaagatc gacaggaacc
     3181 accagccgtt gtccggcgtg aacgccagca cgccactggc cagggagaaa ttgatgacgg
     3241 tgccgataaa gacctttcgc cgtccgtaca catccgcgag ccggccccag atgaccgaac
     3301 ccaggatggc gccgacgccc gaggacaata agatgatcgc cgactgcccg aacgtcaggt
     3361 gccagggctt gatgataaag gcgagcacga agccgatgag atagtaatcg aagaactcca
     3421 gcatgtcgcc gatgattgcc gcggcgatga tcttgatctg gttgctcgtc agtgacgtgg
     3481 cccggtcgag gcggtcgaac atcttgaatg gctccctatc cccggggaag ccccggttcg
     3541 ttgcggataa cggtgcctga attgatggtg acgtcaaccc acgcgaggca cagtcatcgg
     3601 ttttgccggc tttgggagta gaggtggttc ggcgggcaat tcgccggtgt cgtgaggatt
     3661 ggcgatggga attggtgcga ctgcggaaat tggtgggacg gatctgctta gccgaagtcc
     3721 ttgggtcgca cggcggcatc taaccgtgag cgaatatccc cgcatgggtg aggcgggcat
     3781 acggccggag gacgaccggg tcgagctcat cgaaggcgaa ctgatagcga tggcgcccat
     3841 cgggagcggg cattcccaca cagtcatttc ggtgactcat ctttggtcgg tcgcgtggac
     3901 cagaggggct cggtgacgat tacacgtcga tcgcaagggt tggacatagc ggcagcatcg
     3961 agctggtggg cctgccgggt gtcatcatcc ccgctacagc gcttttcggc taagccgttc
     4021 gcgcttgatg cggcttgcgg ggctagacca cagtcagcct caccgtgcag gccgcaaatg
     4081 gtccaccttt tatcacgtga ttcacacgcc ttggacgttc cgcccagcgt gacctcgctt
     4141 taaggacgtc aggccgaacg acttcgcccg acgcttgtca gtgcggcttc gaatcgcctc
     4201 gcgacctcgt tcgccatgga tgtcggggtg gccgcgaaac ccaccaccaa cccagtcatg
     4261 cgtggctttc cgggatagta cgcccgcagc ggcgacactg ccagatcccg cgatcggcac
     4321 gcccgaacgg cggtggcttc atccacgtca tccggcagcc tggcaaccat atgcagcccc
     4381 cccggtgctg cgagcggcgt cgcatggcgg accaggcgtt tcatcgcctc cagcagcgcg
     4441 tcgcggcggc gagaatattc ggtccgcatc tttcggatat gaggcgacag caggcccagg
     4501 cgcatgaact ccgccagcgt cgcctgggtg aacgcgtccg tgccgcggtc ggtcagcgtg
     4561 cgaagccgga caaacgcggg aaccagtggt gccggcacga tggcgaaacc catccgcagc
     4621 gccggggcga gggtcttgct gaaggtgccg cagtagatca cctgcccggc tttatccagc
     4681 gtcgcgagcg gcgggagagg tttgccttcc cagcggaatt cggagtcgca atcgtcctcc
     4741 agcacccatg ccttcgcgcg ccctgcccag tccagcagcg ccaaccggcg cccgatgggg
     4801 agcgccccgc ccaagggtgt ggcatgcgag ggggctacca acgccatccg ggcatccggc
     4861 gcgattcgga cgccggcggc aacgtctatt ccctccgcgt cgcttggtat cggcacgatc
     4921 cgcgctccgg ccgcgagcag cgcaccccgg ccggcaatat acccgggatc ctcgacccaa
     4981 accggatcgt ccgggtcgag cagcaggtcg gctgcggttc gcagcgcctg ttgagtgccg
     5041 ccggtcacca cgatgcagcc aggatcggcg accaggccac gcgtcgcggc cagatgcgcg
     5101 gcgatctgct cgcgcagcat caacagtccc tgcggtggcg ggtaactgga tagctccccg
     5161 gagagcggtt tcaggacgcg ggacgcgcaa cgggcccacg ccttgatcgg aaacagatcg
     5221 ggtgccggca ggccgccggc cagcagcagt ccgagcaccg ggtcccgggc ggtggcagtt
     5281 gcggaaatcc cggcaaggtg cgtaccgcga gccgatagtg cgacggcggc cgacgacggc
     5341 gtcgcgaccg gggcgggcgc ctggtccggt aggtccgtgg ttatataggt gccgctgccg
     5401 gtgcggccgc ggacatagcc ctcggcggca agacgctcat aggccagcac cacggtttgg
     5461 cgagcgaccc cgagttcgtt cgcgagagaa cggctaggcg gcaggcgccc gccgccaggg
     5521 agtgcacccg tcaagatgct gtgacgcaag tggtcgaaga cggcggcctg ccggggtcgg
     5581 tctggtgcaa gcatgctgcg agggcgctac cgattggtcc aattgccaat ctagaccggg
     5641 tttggcgtta tccacaagag tacacgcctt tggcgcggcg ttgggcgctt gtccccagcc
     5701 gcgctgcctg ttgcgttccg agagttgtta gaagccttcg cgctccagcc gcttgcgttc
     5761 cagcttcctg gcacggcgaa cggcttccgc tgcctcacgc gcccgtcgct cggacggctt
     5821 ctcgtaatgg cgccgcagct tcatctcgcg aaagataccc tcccgctgca ttttcttttt
     5881 gagcgccttg agcgcctggt cgacattgtt gtcacgcacg agaacttgca cttggcggtc
     5941 tgctcctatg aatgcgcgca ccggcgtgca cgcggtcatc cgtcaccggg ggattccagc
     6001 gcgggggctg gcgctataac atggccgtgc gtacttacaa gtccattctc cgctttcagg
     6061 gatcgacgca cacatggcca ttctgaaaat tgcccggatg ggccatccgg tcttgctgca
     6121 gaaagccacg ccggttgccg atccgggcgc ccccgagatc cgacggctcg ttgacgacat
     6181 gatggaaacg atgatggatg cacccggtgt cggcctggcc gcgccacagg tttatcagcc
     6241 tctgcgattg ttcgtgttcc gcgtgccgga aggccgcgaa acgcgggatc cggacgacat
     6301 cacgctggcg aacaccgttc tgatcaaccc cacgctggag ttgatcggcg aggaacgcgc
     6361 cctgggctgg gaaggctgtc tatcgatccc cggtctgcgg gccgcggtac cgcgggccag
     6421 gcgcatccgc tatcgcggcg tggactgcga cggcaacgtc acggaacgcg aggcgaccgg
     6481 ctttcacgcg cggatcgtcc agcatgaata cgaccacttg gacggcatcc tgtacactat
     6541 gcgtatgtcg gacttccgcc atttcggctt taccgacgaa ctcgatcgcg cgggaacgca
     6601 aggatgattc ggacacctga acgaatgccg gagcgcgatg cggcgatcct ggcgatgctg
     6661 ccgcatgtgg cattcgacgg ctggacgaaa cgtgcgctgc gggcgggcgt ggccgatgcc
     6721 ggtatgccgg cggacgaggc cgagttgctg tttccgcttg gcaccgtgga tatgatcgag
     6781 acgttctgcg atctcgcgga tcggcgaatg gaggcggctt ccgccgaact ggccgaaacc
     6841 agactgtccg cccgcgtgcg tgcggtgatc gccctgcggc tgcggcagaa ccgtcccttc
     6901 aaggacgcga tccgccgtgc gctggcgatc ctggcgctgc cgcagaacgc cagggcggcc
     6961 gctttctgct ccgcccgaac ggcggatgcg atttggcacg ccgccggcga ccggtctgcc
     7021 gacttcagtt ggtacacgaa gcgggccatc atgactgccg tttttggcgc caccgtgctg
     7081 ttctggttgc gcgacgggtc cgaggatgat gccgatacgc tggcttttct tgaccgcagg
     7141 ctggcggcga tcggccggat gggacggctg cgtggtcgcg ccgaccggct ggttgcccgg
     7201 ctacccagat tgccacttcc caggttgccg cgcgtgcgaa tgccgccctg ccagccggcg
     7261 gcaacaggag ttggtgacac cggttgatgg agtgtctatc tctgcgccaa ctccggccgg
     7321 cgttccgttg gccggcctat agcgcaggcc catgaccgac acccctatcg agcgtatccg
     7381 caatttctcc atcatcgccc atatcgacca tgggaaatcc accctggccg accggctgat
     7441 ccaggtcacc ggcgggctga cagcgcggga gatgaccgcc caggtcctgg acaacatgga
     7501 cctggagaaa gaacgcggca tcaccatcaa ggcccagacc gtccgcctga gctatacggc
     7561 gaaggacggg ctgacttacg agctgaacct gatggacacg cccggccatg tcgatttcgc
     7621 ttacgaggtc agccgtagcc tggccgcgtg cgagggcagc ctgctggtgg tcgatgccag
     7681 tcaaggcgtc gaggcgcaga cgctggccaa cgtttatcag gcgatcgaag ccaaccacga
     7741 gatcgtgccg atcatcaaca agatcgacct gccggccgcc gaaacagaac gtgtgaagca
     7801 gcagatcgag gatgtgatcg gtctggacac ctcccacgcg gtggagatca gcgccaagac
     7861 cggtctgaac atcgaggggg tgctggaagc gctggtgaca cgcctgccgg cgccaaaggg
     7921 cgatggcaaa gcaccgctga aggcactgct ggtcgatagc tggtacgatc aatatctggg
     7981 tgtcgtcatc ctggtccgga tcaaggacgg gcggatgaag cgcggtcagc gcatccgcat
     8041 gatgtcctcg ggtgcggtcc attcggtcga gcaggtgggc gttttcacgc cgaagatgat
     8101 cccgacagac gatttgggac cgggcgagat cggcttcatc accgctgcga tcaagactgt
     8161 tgccgattgc aacgtcggcg ataccattac tgacgaccgc atgccggcga ccgcggcgct
     8221 gccggggttc aagccatcca taccggtggt ctggtgtggc ttgtatccaa tcgacgccga
     8281 tgacttcgag aagttgcgcg acagcctggg aaagctgcgg ctgaacgacg ccagcttcca
     8341 ctatgacccg gaaacctccg ccgcgctggg gttcggattt cgctgcggct tccttgggct
     8401 gctgcacctg gagatcatcc aggagcggct gacccgcgag ttcgacctgg aactcatcgc
     8461 caccgcgccg tcggtggtct accacgtcta caagaccaat gggcagatgc aggaactcca
     8521 caacccggtc gatatgccgg acggttccgt gatcgagcat atcgaggagc cttggatcaa
     8581 ggccaccatc atggtgccgg acgagtatct cggcagcgtg ctggggctgt gcacggagcg
     8641 gcgcggccag caggtggacc tgacctatgt cggcaaccgc gcgatggcgg tgtaccgcat
     8701 tccgttgaac gaagtcgtgt tcgacttcta cgaccggctg aagtccgtat cccgcgggta
     8761 cgccagcttc gactacgcga tggacggata tgccgaaagc gaccttgtgc ggatctccat
     8821 cctggtgaac ggcgacctgg tggacgcgtt gtccttcatc gcgcaccgag cggcagcgga
     8881 aaaccggggg cgccagattt gcgcccgtct gaaagacctg atcccgaagc agttgttcaa
     8941 gatcgccatc caggcggcga tcggtagccg ggtggtggca cgcgagacga tcagcgcctt
     9001 gtccaaggac gtgaccgcca aatgctacgg cggcgacatt tcgcgcaagc gcaagctgct
     9061 ggaaaagcag aaggagggca agaagcggat gcgccagttc ggcaaggtgg aaatcccgca
     9121 gtcggcattc ctcgcggcgc tcaaaatgga tgcctgatta gggtttgcgt gtggcgtccg
     9181 gtctggtatt gaccgggcat cgtgtccggt gggggcgcat cgcccttggg gcatgtccgt
     9241 gtggggcagg cccatctggg gcgcgttctc cctgggcgtg gtcttgggat gccggagaga
     9301 tggccgagtg gtttaaggcg cacgcttgga aagcgtgtgt gcgtgaaagc gtacccaggg
     9361 ttcgaatccc tgtctctccg ccacgacccc cgacggtgtc cggcaaaagc cagatgggat
     9421 ccacgagtct gacagaattt cggacgttac acgggctgcc aggcacgccc gatcgattcc
     9481 cctggccact gccggacttg cctgcgactt gcaccggctt cgcgatctcc tgaagagttc
     9541 gctcgtcgaa cggctggttg tcctgagcgg tccagctgat tctccggtcc agctggtttt
     9601 gatgatccga tcccgccgg
//
LOCUS       DASWQN010001034         3411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_232094, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001034 DASWQN010000000
VERSION     DASWQN010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232094"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1917
                     /locus_tag="VGC82_17665"
     CDS             46..1917
                     /locus_tag="VGC82_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEY0425151.1"
                     /translation="MEAPLMHLALVGTPNSGKTSLFNALTGSRQKVANYPGVTVERKE
                     GAFVTPLGRQVSLVDLPGTYSLRGRSPDEEITRDIVLGRTAGEALPDLVLCVADSTNL
                     RLTIRLLLELKSTGRPLILVLNMFDIATRRGVTVDVPRLSEALGVPVVTSIAVRKGGT
                     AELLRLTDEIAAQAQPADRQNLWEPLSVAQLRATQREADRIIAAAVSLPARPDSWTAR
                     IDAVVLHPVAGLVILAAILFVMFQAVFAWAQPLMELLSIGFAALGQLVHDTLPAGLLQ
                     SFLQNGVISGVGSVIVFLPQIVMIFLFILLLEDFGYMARAAFLMDRIMGGAGLHGRAF
                     IPLLSSFACAIPGIMATRVIDNRRDRLTTILVAPLMTCSARIPVYTLIISAFIPAEQV
                     WGWINLRGLVMFGLYAAGIISALGVSFLIKFFMWRDYAPAPFMLELPDYKMPRARGIA
                     IGVYTRAKMFLQRAGTTIFAMMVMIWFLASFPLPPAGAEGPAINYSLAAMIGKALEPL
                     LAPLGFNWQIAVALIPGMAAREVAVAALGTVYAIEGGKEAADQIGQALAAKWSLATAL
                     SLLAWYIFAPQCASTLAVIRRETGSWKWMAVTFGYMLALAYAASLATYNIAVALGAG"
     gene            2014..2646
                     /locus_tag="VGC82_17670"
     CDS             2014..2646
                     /locus_tag="VGC82_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425152.1"
                     /translation="MKRILWSLPAILCLMTAAPASAQEKVRINPTDPQPTCTMCPGTY
                     IPAAELESYTNKAVVEKLVDQQVRDVDIGKAHIGIGMVHRGKLEKPAPDSVAEHDHVS
                     EVYHVISGAATLVLGPDIVNRQRRPATLRTVVEFNGPGNNGSEVRDGVAHEIKAGDVV
                     VIPAGTGHWFTRIEDHIDYLMIRIDPDKLTPLRSETQSQEYLAKPAKRGE"
     gene            complement(2699..>3411)
                     /locus_tag="VGC82_17675"
     CDS             complement(2699..>3411)
                     /locus_tag="VGC82_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425153.1"
                     /translation="SGQATDDARLRQIVEPVLSAGALSVKSAQIPFNIRDGRLRVGAT
                     ALDAKDARVIISGGYDIPADQADIRVMLASTATGQVTSRPEIQLFAAGSPDALARTVD
                     VAALSSWLAVRAIDRETRRLDSIERGDPLQAFPASIPPAAAAPPSLRAPDAAPPEQPL
                     SEAPVQRRPGPKPRISAPRPPGSPNPNASVLSQHVAPLPPPIEVRPAPGAARAPRLKP
                     RPPLMLTPPLSEPPRSGF"
BASE COUNT          556 a         1134 c         1128 g          593 t
ORIGIN      
        1 cgaggcgatg gccatcattg tcgcctgacg aaacggtgca gcgccatgga agctccgttg
       61 atgcatcttg ccctggtggg cacgcctaac agcggcaaga cctcgctgtt caatgcgctc
      121 accggcagtc gccaaaaggt cgcgaactat cccggcgtca ccgtcgagcg caaggagggg
      181 gcattcgtca cgccgctggg acgccaggtt tccctggtcg acctgcccgg cacctactcg
      241 ctgcgcggcc gcagcccgga tgaggagatc acccgcgata tcgtgctggg acgcacggcc
      301 ggcgaggcgc ttccggacct cgtgctttgt gtggccgatt ccaccaatct gcggctgacg
      361 atccgtctcc tgctcgagct caagagcacc ggccggccgc tgatactggt gctcaacatg
      421 ttcgacatcg ccacccgccg cggcgtgacc gtcgatgtcc cgcggctgtc cgaagcgctc
      481 ggcgttcccg tcgtcacgtc gatcgcggtg cgcaagggcg gcaccgccga gttgctgcgc
      541 ctgaccgacg agatcgcagc gcaggcgcaa ccggcggacc ggcaaaatct ctgggagccg
      601 ctcagcgtcg cgcaactgcg cgccacccag cgcgaggccg atcgcatcat cgccgcggcg
      661 gtgagcctgc cggcgcggcc cgattcctgg accgcgcgga tcgacgcggt ggtgctgcac
      721 ccggtggcag ggctcgtgat cctggcggcg atcctgttcg tcatgttcca ggcggtgttc
      781 gcgtgggcgc agccgctgat ggaattgttg tcgatcggct tcgccgcgct cgggcaactg
      841 gttcacgata ccctgcccgc gggcctgttg cagagtttcc tccagaacgg cgtgatctcc
      901 ggcgtcggca gcgtgatcgt gttcctgccg cagatcgtca tgatcttcct gttcatcctg
      961 ctgctggaag atttcggcta catggcacgg gcggccttcc tgatggaccg catcatggga
     1021 ggcgccgggc tgcacggccg cgccttcatt ccgctgttgt cgagtttcgc ctgcgcgatt
     1081 ccgggcatca tggcgacccg ggtgatcgac aatcgccgcg accggctgac caccatcctg
     1141 gtggcgccgc tgatgacctg ttcggcgcgg atcccggtct acaccttgat catttccgcc
     1201 ttcatccccg ccgagcaggt gtggggctgg atcaatctgc ggggacttgt gatgttcggc
     1261 ctctatgccg ccggcatcat cagcgcgctc ggcgtctcgt tcctgatcaa gttcttcatg
     1321 tggcgcgact acgcgccggc gccgttcatg ctggagctgc cggactacaa gatgccgcgc
     1381 gcccgcggca tcgccatcgg cgtctacacc cgggccaaga tgttcctgca gcgggccggc
     1441 accacgatct tcgcgatgat ggtgatgatc tggttcctgg cgtcgttccc gctgccgccg
     1501 gccggcgcgg aaggccccgc catcaactac agcctggccg cgatgatcgg caaggccctg
     1561 gagccgttgc tggccccgct cggcttcaac tggcagattg cggtggcgct gatccccggc
     1621 atggcggcgc gcgaggtggc ggttgccgcg ctcggcaccg tctatgcgat cgagggcggc
     1681 aaggaagccg ccgatcagat cggacaggcc cttgccgcca aatggagtct ggcgaccgcc
     1741 ctgtccttgc tggcctggta catcttcgcc ccgcaatgcg cgtcgacact cgccgtgatc
     1801 cgccgggaga ccggaagctg gaagtggatg gccgtcacct tcggctacat gctggcgctg
     1861 gcctatgccg caagccttgc gacctacaac atcgccgttg cgctcggcgc cggataacgt
     1921 ccgtctccct gcccggcgtc tatggtcagg catgaagacc ttggcaaagc ggcaggtctg
     1981 tgcgtgcatg accccgacaa cagcgaggtg gtcatgaaac gtattctgtg gagcctgccg
     2041 gcaatccttt gcctcatgac agcggcaccg gcgtccgccc aggaaaaggt gcggatcaat
     2101 cccaccgacc cgcaaccgac ctgcacgatg tgtcccggca cctatattcc ggcagccgaa
     2161 ctcgaaagtt ataccaacaa ggccgtcgtg gagaaattgg tcgatcagca ggtccgggat
     2221 gtagacatcg gcaaggcgca catcggcatc ggcatggtcc atcgcggcaa gctggaaaag
     2281 ccggcgccgg attccgttgc cgagcacgac catgtcagcg aggtctacca cgtgatctcc
     2341 ggcgcagcca cgctggtgct tgggccggat atcgtgaacc ggcagcggcg cccggcgacc
     2401 ctgcgcacgg tggtcgagtt caatggcccc ggaaacaatg gttcggaggt tcgcgacggc
     2461 gtcgcccacg agatcaaggc cggcgatgtc gtggtgatcc cggccggcac gggtcactgg
     2521 ttcacccgga tcgaagacca catcgactat ctgatgatcc ggatcgatcc cgataaactg
     2581 acgcccctca gaagcgagac gcaatcgcag gaatatctgg cgaagccggc caagcggggc
     2641 gagtgaaact ttaccccgcg gtgacgctgc cttcgtttcg cccagccggg ccgatgcgct
     2701 agaaaccgga tcgcggcggc tcggacaacg gcggcgtgag catcagcggc ggccgcggct
     2761 tcagcctcgg cgcccgcgcc gcgcctggag cggggcggac ctcgatcggc ggcggcagcg
     2821 gcgcaacgtg ctggctgaga acggatgcat tcggattggg tgaaccgggc ggacgcggcg
     2881 cgctgatcct tggcttcggg ccggggcggc gctgaaccgg cgcttcggaa agcggttgtt
     2941 ccggcggggc ggcatccggc gctcgcagcg aaggcggcgc ggcagccgcg ggcgggatcg
     3001 aggccggaaa cgcctggagc ggatcgccgc gctcgatcga atccagcctt cgcgtttcgc
     3061 gatcgatggc cctcaccgcc agccatgatg acagcgccgc gacgtcgacc gtccgggcga
     3121 gcgcgtcggg cgaacccgcc gcgaacaatt gaatttcggg acggctggtc acttgacccg
     3181 tcgcggtcga agccagcatc acgcggatat cggcctggtc ggcgggaata tcgtatccgc
     3241 ccgatatgat gacgcgggca tccttggcat cgagcgcggt ggccccgacg cgaagccggc
     3301 catcccggat attgaacgga atttgcgccg acttcaccga caacgcgccc gccgacaaca
     3361 caggctcgac gatctggcgc agcctggcat cgtcggtcgc ctgcccgcta t
//
LOCUS       DASWQN010001035         2929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2608134, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001035 DASWQN010000000
VERSION     DASWQN010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2608134"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1218)
                     /locus_tag="VGC82_17680"
     CDS             complement(166..1218)
                     /locus_tag="VGC82_17680"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0425154.1"
                     /translation="MRIIGCILSAMLLSLPLTARAQTEAVYPIGRFAFEDGGEIQDMK
                     VGYVTWGTLNAARSNAILLVPGTSGSRHSYDGHIGPGKTFDTDKYFVIGADPIGGGTS
                     SQPKDGLGTAFPHYTIRDMVRAQHEMIEKRLGITHLLAVGGASMGSFQTVEWGINFPD
                     AMSGLIMIVPAARSDHHFAAVVDAFEATVTLDPKYQGGKYTENPVEGIRRAALIYFPW
                     TSSDEYLASMNEDAYEAGKKAAGDAWVKNWDANSMLWRYAASRNFDASKPFGGDMMKA
                     LAQVKAPVLLLPSATDRTIPGYLTRELYRGLRGNVTYAEIPSVRGHSAGGQPPGTAEY
                     AYVSDKIRGFLAGLGS"
     gene            1455..1726
                     /locus_tag="VGC82_17685"
                     /pseudo
     CDS             1455..1726
                     /locus_tag="VGC82_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661703.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1737..2159)
                     /locus_tag="VGC82_17690"
     CDS             complement(1737..2159)
                     /locus_tag="VGC82_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY0425155.1"
                     /translation="MKQAIHTLHVPTAGQALYDITRPIRAWVQDQGIGTGLLTVWCRH
                     TSASLIVQENADPDVQADLLDFFRRIVPEASQYRHNAEGPDDMPAHIKAALTQTGLSI
                     PIQHARPVLGTWQAIYLFEHRTEPQTRSIALHILGAAD"
     gene            complement(2200..>2929)
                     /locus_tag="VGC82_17695"
     CDS             complement(2200..>2929)
                     /locus_tag="VGC82_17695"
                     /inference="COORDINATES: protein motif:HMM:NF040115.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HEY0425156.1"
                     /translation="ALTTAQLPALATGALVVLTTAETAALTTAQVASLTTAQAAALTT
                     AQIAAMTTAQVAALGTANVKALATNQVAALTTAQVAALTTAEASTLTSAQVAALTTTQ
                     AGAITTAQAAALSTTAIPGLASAQFAALTSAAVLALTTAQFGALGTDAIAALTTAETA
                     ALSTADIVALSTKQLVALTTADIVALTTGQMAALTTSQIPAFSTSQIPAFTTAQFNVL
                     SSSQIPAFTSTQLGAMTTAQFSAL"
BASE COUNT          485 a          873 c          940 g          631 t
ORIGIN      
        1 aatgaatatc tgtgacgcca tttacctgac ctccgatcta tgcaggcgga agttaaggtg
       61 gtccgccaga tacatggtgg tggtcggtta ttcgctgacc tgtttagact gcgatggttt
      121 ttgtaaatgc accttatacc gagcgggatt ttcgtgcccg taaacctaac tccctagccc
      181 ggctagaaat cccctgatct tgtcgctgac ataagcatat tccgccgtcc ccggcggctg
      241 gccacccgcc gaatgtccgc ggacggaagg aatttcagcg taagtaacgt ttccgcgcag
      301 cccgcggtac aattcccggg tcagatagcc tggaatcgtc cggtccgtcg cgctgggcag
      361 cagcaacacc ggcgccttga cttgcgccag cgccttcatc atgtcgcctc cgaacggctt
      421 cgaggcatcg aagtttcggc tggcggcata ccgccacaac atgctgttgg catcccagtt
      481 cttcacccac gcatcgccgg cggccttctt gccggcctcg tacgcgtcct cattcatcga
      541 cgccagatat tcatcgctgc tggtccaggg gaaatagatc agtgccgctc gacggatacc
      601 ctcgaccggg ttctcggtat attttccgcc ctgatatttc ggatcgagcg tgaccgtcgc
      661 ctcgaacgca tccaccacgg cggcgaaatg atggtcgctg cgggcagccg gcacgatcat
      721 gatcagaccg ctcatggcat caggaaagtt gattccccac tcgaccgtct gaaacgagcc
      781 catcgacgcg ccgccgaccg cgagcaggtg ggtaatccca aggcgttttt cgatcatctc
      841 gtgctgtgcc cgtaccatgt cgcggatggt gtagtgggga aacgccgttc ccaacccgtc
      901 ctttggctgc gacgaggtac caccgccgat cgggtcggcg ccaatgacaa agtacttgtc
      961 cgtatcgaat gtttttcccg gaccgatgtg tccgtcgtag ctgtgccggc tgccgctggt
     1021 gccgggaacc agaaggatcg cgttgctcct ggccgcgttc agcgtgcccc aggtgacgta
     1081 tccgaccttc atgtcctgga tctcgccgcc gtcttcgaag gcaaaacgac cgatcggata
     1141 gaccgcttca gtctgcgccc gcgccgtcag cggcagggac agcagcattg cggataaaat
     1201 gcatccgata attctcatgg ttccttcccc caggcccgaa catttcaata cactttgaca
     1261 taaggacggc tgcctcgcca cagtgggatt gcaatggtcg ttggcacgcc gggcgaaagg
     1321 acgaacgatg aagacgggtt tggccatgac cttgtcccgc gatcacgacc taatcgcacc
     1381 ggacggatca gaggtgcggg tcctgttgtc gctcgccgct ggcaacattt tacgtttccc
     1441 gctcggcagg tctcctgcgc ggtccgtcac cggtccatcg aggagataat gcccgtcatg
     1501 atctgaacga gatcctggtg attgcgttcg ccagcatgct gtgtggtggg aggattgctc
     1561 cgagatggcg ctgttcgggc gtgccaagga aggtttccta cggtaattcc tccgcctcaa
     1621 gcgcggcatc cccagccatg acacgttcag ccgattgttc cgcttgctgg atcccacgca
     1681 gtcccaggcc tgctttctgc gtttcatggg cgagttcgcc gccgcggcga cagtcgctag
     1741 tctgccgcac ccagaatatg tagtgcgatg gagcgcgttt gcggttccgt ccggtgctcg
     1801 aacagataga tcgcctgcca tgttcccagt accggccttg cgtgttgaat cggtatggac
     1861 agaccggttt gtgtcagcgc cgccttgatg tgggcgggca tgtcgtccgg cccctcggca
     1921 ttgtgtcggt actggcttgc ctccgggaca atgcggcgga agaagtccag caggtcggcc
     1981 tgcacgtccg gatcggcgtt ctcctgcacg atcagcgagg ctgaggtatg ccggcaccag
     2041 accgtcagca gtccggtgcc gatcccttga tcttgaaccc aggcgcggat tggccgcgtg
     2101 atgtcgtaga gcgcctggcc ggcggtgggg acatgaagcg tatggattgc ctgtttcatg
     2161 tccctggttt ggggtttccc ccggccatgc gcaacggtgt cataacgcgc taaactgagc
     2221 cgtggtcatc gcgcctagct gagtggatgt aaaagccggg atttggctgc ttgacaggac
     2281 gttgaactgc gccgtcgtga aggccgggat ctggctggtg ctgaaggccg ggatttggct
     2341 ggtggtcagc gctgccatct gtccggtggt cagggcaacg atgtccgcgg tcgtcagtgc
     2401 gacgagctgc ttggtggaca gcgccacgat gtcggctgtg gatagcgcgg cggtttccgc
     2461 ggtggtcagg gcggcgatcg catccgtccc gagcgcgccg aactgggctg ttgtcagtgc
     2521 cagcacggct gccgaggtta gtgccgcgaa ttgggctgac gccagaccgg ggatggcggt
     2581 ggtggacagt gccgccgcct gtgccgtggt gatggcgccc gcctgggtgg ttgtcagggc
     2641 cgcgacctgg gcggatgtca gtgtggatgc ctcggctgtc gtcagggccg cgacctgggc
     2701 ggtggtcagg gccgcgacct gattggtcgc cagcgccttg acgttcgcgg ttcccagcgc
     2761 ggcgacctgt gccgtggtca tggcggcgat ttgcgcggtg gtcagtgcgg ctgcctgcgc
     2821 tgtcgtcaac gaggcaacct gtgcggtggt gagggcggcg gtttccgccg tcgtcagcac
     2881 gaccagcgcg ccggttgcca gggcagggag ctgtgcggtg gtcagcgcc
//
LOCUS       DASWQN010001036         4086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2474709, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001036 DASWQN010000000
VERSION     DASWQN010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474709"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VGC82_17700"
     CDS             complement(<1..544)
                     /locus_tag="VGC82_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973709.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEY0425157.1"
                     /translation="MPQPDHISKLGVPDRSSLDEDLQQVFVKCVDKLGYVPNVLAAYS
                     LRPQRLRNFMSIYNEIMLSPSGLSKLEREMVAVVVSSANRCYYCLVAHGAAVRQLSGD
                     PELGEMLALNYRIAQLDPRQKLMLDFAWKLTKTPDLVDDSDRDALSQQGLSQEDIFDL
                     AETVAFFNLSNRMASAMDMIP"
     gene            complement(544..1524)
                     /locus_tag="VGC82_17705"
     CDS             complement(544..1524)
                     /locus_tag="VGC82_17705"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEY0425158.1"
                     /translation="MATNKRKVMLPHTMGQQGIDVMQARDDIETIIYPAGISQPELLP
                     RLVDIAGIALSGTPFRQKEINAAAAMQVVARIGVGYDAVEVPALTARRVPLLTAGTAN
                     STSVAEQAFHLIIALAKKNRTLHALVTEDKWAQRHTAPPMELSGKTILIVGFGRIGTR
                     TARRCAAFDMTVLIHDPYIKQDNIRSAGYQIADDLDAALPKADIVCIHCPKSPETVGM
                     FNATRLGLMKRGAFIVNTARGGIIDEPALYKALTSGHLAGAGLDVFEQEPTPPDNPLL
                     SLENVISSPHMAGVTVEAIAAMAVATSLNILSVLDGTPNRENTINPEVYD"
     gene            complement(1537..2295)
                     /locus_tag="VGC82_17710"
     CDS             complement(1537..2295)
                     /locus_tag="VGC82_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0425159.1"
                     /translation="MADLAGKIAWVTGAGTGIGEAAALALAEAGATVVLTGRRPGPLE
                     DVARRINQSGVAHVQPADMTKADQVRKVGDYIRATFNRLDILVNNAGVNIVERHWDKL
                     TPESIDILLQGNLTGALYCVTVALPFMREQKDGLLIHTASMAGRFIGGVSGPIYTVAK
                     HGIVAMSHGLNMQECINGIRSTVLLPGEVATPILDQRPNPVGPEVRARMVQAADCGDL
                     IRYIACLPKHLVMNEVHLSPTHNRGYIAALQRQL"
     gene            2479..3357
                     /locus_tag="VGC82_17715"
     CDS             2479..3357
                     /locus_tag="VGC82_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0425160.1"
                     /translation="MDLSFDLLGNALVSGLLLGGFYAAVTIGVSISFGMLDIVNIAHP
                     AFIILGSYTAYIANSVFGFDPILSSIVLLPVFYLLGVAIYHVYDVSFERRGDASIRGL
                     AFFFGLLFITEVTLVLVFGVDYRLVDAPYIGPSLHFGVIDLPLRLLVPFLVSLALIGS
                     LQLFMSYTFIGRAVQAVAQDPMALRLMAANPSQIKRIAFGLSIASASVAGAILIIIQP
                     VEPSIARDYIGRIFAICVMGGLGSFGGTILAAMILGIMESLTSTFYGPSWSPAVAFGF
                     LLITLAVRPAGLFGRL"
     gene            3357..>4086
                     /locus_tag="VGC82_17720"
     CDS             3357..>4086
                     /locus_tag="VGC82_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0425161.1"
                     /translation="MRNVRFVLLALLAAAATFAVARFIDNAYVYFAGYVVLQYIVLAT
                     AWNILGGYTGYVNFGTAAFFALGAYTTVVLHKTVPSMPLPAMMLVGGLISGVIGLGMG
                     YLTLRLRGTFFSIATLAMAVVAQTLITNWNFVGGSRGAYIIRPAESPIFTGDYIQYLF
                     VVMLLLSVMSVSIARAIENSRLGYGFSAIRDDEVAAEASGVPTLRLKLIATTLSGGLM
                     GMAGAPLPYYVTYLDPASGFSLSYA"
BASE COUNT          705 a         1208 c         1216 g          957 t
ORIGIN      
        1 taggaatcat atccatcgcc gatgccatac ggttggacag gttgaagaat gcaaccgtct
       61 cggccaggtc gaatatatct tcctggctca gcccctgttg cgacagggcg tcgcggtcgg
      121 agtcatcgac cagatccggc gtcttcgtca gcttccaggc gaaatccagc atcagctttt
      181 gccgcggatc gagctgtgcg atccgatagt tcagcgccag catctccccc agctcaggat
      241 cgccggacaa ctggcgcact gcggctccgt gcgccaccag gcaatagtag cagcggttag
      301 ccgaggatac gacgaccgcc accatctccc gctccagctt cgaaaggcct gagggcgaca
      361 gcatgatctc attgtagata gacatgaagt tacgcagacg ctgcggcctc aaactgtagg
      421 ctgccagcac gttgggcacg taacccaact tatccacgca cttcacaaag acctgctgaa
      481 ggtcctcatc cagcgacgag cggtcgggaa caccaagctt cgatatatga tcaggctgcg
      541 gcatcagtcg taaacttccg ggttgatcgt gttttctcga ttcggcgttc cgtccagaac
      601 gcttaggata ttcaatgacg tagcgacagc catggcggcg atcgcttcga cggtcacacc
      661 cgccatgtgc ggtgatgaaa tcacgttttc cagactcagc aacgggttgt ccggtggcgt
      721 gggctcctgc tcgaagacat ccaggccggc gccagccaga tgtcccgagg tgagggcctt
      781 gtacagcgca ggctcgtcga taatgccgcc acgagccgtg ttgacgatga atgcgccccg
      841 tttcatcagc ccgaggcggg tggcattgaa catcccaacc gtctcggggc ttttcgggca
      901 gtgaatgcaa acgatgtcgg cttttggcag cgcagcgtcg aggtcatcgg cgatctgata
      961 tcccgcggag cggatgttat cttgtttgat gtaggggtcg tggataagta cggtcatatc
     1021 gaaggcggca caacgccggg cagtccgtgt gccgatgcga ccaaagccca ctatcaggat
     1081 tgttttccct gacagttcca tcggcggcgc ggtatggcgt tgcgcccatt tgtcctccgt
     1141 gaccagcgca tgcagggtcc gattcttctt cgccagcgcg atgatcaggt ggaacgcctg
     1201 ttcggcgacc gatgtggagt tggccgttcc cgcggtcagc aacggcaccc gcctcgcggt
     1261 cagtgccgga acctcgacgg cgtcgtagcc aacgccgatg cgcgcgacga cctgcatggc
     1321 cgcggctgcg tttatttcct tttgccggaa cggcgtgccc gacagggcga tgccggcgat
     1381 atcgaccagg cgtggcagca gttcgggttg gcttatgccg gcgggatata tgatcgtctc
     1441 gatatcgtcc cgcgcctgca tcacgtcgat gccctgctgg cccatcgtat gcggcagcat
     1501 gactttgcgc ttattggtcg ccatgacgga cgtttcctaa agttggcgtt gcaaggcggc
     1561 gatgtagccg cggttgtgcg tcggcgagag atgcacctca ttcatcacga ggtgcttcgg
     1621 caagcaggcg atgtagcgta tcaggtcgcc gcaatcggca gcctggacca tgcgcgcccg
     1681 aacctcggga ccgactggat tgggccgttg atccagaatc ggtgtcgcga cctcgcccgg
     1741 cagcagcacg gtcgagcgga tcccattgat gcactcctgc atgttgagcc cgtggctcat
     1801 agcgacgatg ccatgtttgg cgacggtata gatcggaccg ctgactccac cgataaagcg
     1861 gccggccatc gaggcggtat ggatcagcaa cccgtccttt tgttcccgca tgaagggcag
     1921 cgcaaccgtc acgcagtaca gcgcaccggt cagatttccc tgcaacagga tgtcgatgct
     1981 ctccggtgtc agcttgtccc agtgacgctc gacgatattg acccccgcat tgttcaccag
     2041 gatatccagc cggttgaagg ttgcgcggat atagtcgccg accttgcgga cctggtcggc
     2101 ctttgtcatg tcggctggct gaacatgggc aacaccggat tggttgatgc ggcgagcaac
     2161 gtcctccagc ggacctggtc gccgcccggt caggacgaca gtcgctccgg cttcggccaa
     2221 cgccagcgct gccgcctcgc cgatgcctgt gccggctccg gtcacccagg cgatctttcc
     2281 cgccagatcc gccatgattc tgttcctcct gtcgttgccg ccgccattct ttagtttcga
     2341 ccacaccagg gcaagagcca gcggcacaat tgccagccgt acagttgcca ggggtacgat
     2401 tgccaccggt acaattggtg gctagccaaa ctcgttggct ttcgtcacat tggcgccggg
     2461 aacgtcataa gcgagccaat ggacctgtct ttcgatctcc tgggcaatgc ccttgtgtcc
     2521 gggctcttgc tgggcggctt ctacgcggcg gtgacgatcg gagtgtcgat ctccttcggg
     2581 atgctcgaca tcgtcaatat cgcgcatccc gcctttatca tcctgggttc ctacaccgca
     2641 tacatcgcca acagcgtgtt cggattcgat ccgatcttgt ccagcatcgt tctgctgccg
     2701 gtgttctatt tgttgggtgt agcaatctac cacgtctatg acgtttcttt tgaacggcga
     2761 ggcgacgcct cgatccgcgg tctggcgttc tttttcggtc ttctgttcat taccgaagtg
     2821 acgctggtgc tggtttttgg cgtcgactat cggcttgtgg acgcccccta tatcggtccc
     2881 agcctgcatt ttggggtcat cgatctgccg ctgcggcttc tcgttccatt cctcgtttcg
     2941 ctggccttga tcggttcgtt gcagttgttc atgtcctaca cgttcattgg acgagcggtg
     3001 caggccgtgg cacaggaccc gatggcattg cggctgatgg cggccaatcc cagccagatc
     3061 aagcgaatcg cctttggtct ttcgatcgcc agcgcctcgg ttgccggcgc gatcctgatc
     3121 atcattcaac cagtcgagcc gtcgatcgcc cgcgactaca tagggcggat attcgcgatc
     3181 tgcgtcatgg gcgggttggg aagctttggg ggaacgatcc tcgcggcaat gatcctgggt
     3241 atcatggaaa gcctgacctc gacattctat ggaccgtcct ggtcgccggc cgttgccttc
     3301 gggttcctgt tgatcacact cgcggtgcgc cccgcgggct tgttcggccg cctttgatgc
     3361 gcaacgttcg ctttgttctg cttgccttgc tggcggcagc cgcgacgttt gccgtcgcca
     3421 ggttcataga caacgcatat gtgtatttcg cgggatatgt tgtcctgcag tatatcgtgc
     3481 tggcgaccgc ctggaatatc ttgggcggtt acaccgggta tgtgaacttc ggcacggctg
     3541 cgttctttgc tctcggcgca tacacgactg tcgtcttgca caagaccgtc ccatcgatgc
     3601 cgttgccggc aatgatgctg gtgggaggtc tgatttcagg cgttatcggc ctgggcatgg
     3661 gctatctgac gttgcggttg cgcggcacgt ttttctcgat tgccacactg gcaatggctg
     3721 tcgtcgcgca gacgctgata acgaactgga atttcgtcgg cggatcgcgc ggcgcctata
     3781 tcattcggcc ggcggagtcg cctattttca ccggtgacta tattcaatac ctgttcgttg
     3841 tcatgctgct gctatccgtg atgtcggttt cgatcgcgcg tgctatcgaa aactcgcggc
     3901 ttggatatgg cttctccgcc atacgcgacg atgaagtggc ggccgaggct tcgggtgtgc
     3961 cgacgttgcg gctgaagttg atcgcgacaa ccctgtcggg cgggttgatg gggatggccg
     4021 gcgcaccgtt gccctattat gtgacatacc ttgatcctgc ctccggtttc agcctgagtt
     4081 atgccg
//
LOCUS       DASWQN010001037         4539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2474757, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001037 DASWQN010000000
VERSION     DASWQN010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474757"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VGC82_17725"
     CDS             complement(<1..603)
                     /locus_tag="VGC82_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0425162.1"
                     /translation="MSAIRVIATGLRFPEGPVAMKDGSIVLVEIERQTVTRVHPDGRT
                     EVVAHTGGGPNGLAVGPDGAFYVCNNGGFQWRTELNLLRPAGPASDYTGGRIERIDPI
                     TGTVAVLYDRCGPHKLLGPNDIVFDGLGGFYFTDLGKTRARDRDWGGVYYALADGSRI
                     TEVAHPILTPNGIGLSPDGKTLYVAETETARLWAFDVLAPG"
     gene            979..2298
                     /locus_tag="VGC82_17730"
     CDS             979..2298
                     /locus_tag="VGC82_17730"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0425163.1"
                     /translation="MRTLANHPNTIVLSFATTLLGGLLAVGLIVPPLFGMLLAAAVAV
                     GICFLAFCFPTIVCVVWLLVTSLSLEMAMHDLVAAESFQPTIAVVKGTEIGLALLCMI
                     RFGPKPDPLSPAWGFLVILAAGLVHGLYPGLSMADSLRSMIGSVAPFVFCFCRVPRSW
                     ADAMIQATKWCPVVVVLAGLPLAVVGIRPLFIDSGGARLAGLGHPAFLAGICLPAIYA
                     CLIELYREGRRGDLLLLVINFLILALTGARAPFVCAAAVTGISLISIRSVVFTARDRL
                     LLVLSALAVMSVLAIFAGDLAELRLFNVFLHETSNLSGRGLLWPSFEDAAAQSPWFGW
                     GSGAGNVVISPDGRIARVLHTWAAHNEYLRIGVEGGLVGEILLIALFAGWALVHTHRL
                     QASDRRIMRLAFLVLAGHAVTDNVLISTPACVMFAFATAVFARKAPE"
     gene            2362..3327
                     /locus_tag="VGC82_17735"
     CDS             2362..3327
                     /locus_tag="VGC82_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY0425164.1"
                     /translation="MRRFFLLLFCALSCAAATAAKAGGIAFVVNSGSATISVIDMTTL
                     KEIQRIPTLREPHHLVLSPDGRSLLVGDTAGNQMMFLDPNTGAVQQRMPVTDPYQMGF
                     SPDGKFLVVNGLLRNQVDVYDAVTLKLLKRFAVVSTPSHLAYSPDSSSVFVSLQDSDK
                     LAAFDLRSLTEKWTVPVGRTPAGVMWLNGKVLVADMGTDYVAVVDPASGKVLEHVQTG
                     KGAHNLFLSPDHKILWVNNRAGSSTMSLDASTLKPIRSYAIPGGPDDMDFAPGGKIWI
                     TRRWAEKVAILDPATGSYETINVGRSPHGLFLNPKAPSPAVLSSR"
     gene            3327..4274
                     /locus_tag="VGC82_17740"
     CDS             3327..4274
                     /locus_tag="VGC82_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0425165.1"
                     /translation="MDDPFDLAAGWLQEHLLIPALYQFGLMNWEDVSYGWALFALYGV
                     AQVAVTLAVCLPLERWRPVERWPDHSAVWVDVAYTLLSRVGVLPLVTYVMFYRAQVTV
                     SGWMTDRGIVPPTLEGLFPILLGQQVLTFVLYAIILDFADYWRHRLSHMFRWWWSLHS
                     LHHAQRQMTFWSDDRNHVLDDLIAFLWFGAVALLIGVPPLQFPLLVLVLRFLESLSHA
                     NVRLSFGWIGERLLVSPRFHRAHHGVLAVAQRSCNYGAVLPWWDILFRTADFSLGYAR
                     TGDPSGEEALATGTWIEQQKAGLRRLARSFGQRRARRGT"
BASE COUNT          715 a         1406 c         1452 g          966 t
ORIGIN      
        1 gcccggtgcc agcacgtcga acgcccacag ccgagcggtc tcggtttcgg cgacgtagag
       61 ggtcttgccg tcgggggaca ggccgatacc gttcggcgtc aggatgggat gagcgacttc
      121 ggtaatgcgc gagccgtcgg ctagtgcgta atagacgcca ccccaatcgc ggtcgcgcgc
      181 cctcgttttg ccaaggtcgg tgaagtagaa cccgcccagc ccatcgaaca cgatatcgtt
      241 cggccccagc agtttatgcg gcccgcagcg atcataaaga accgccacgg tgccggtgat
      301 ggggtcgatc cgctcaatcc ggccgccggt atagtccgat gcgggaccgg ccgggcgcag
      361 caggttcagc tccgtgcgcc actggaaacc gccgttgttg caaacataga acgcgccatc
      421 gggtccgacc gccaagccgt tcgggccgcc gccggtgtgg gcgacaacct ccgtccggcc
      481 gtcggggtga acgcgggtaa cggtctgccg ctcgatctcc accagtacga tggagccatc
      541 cttcatggcg accggacctt cgggaaagcg taaaccggta gcgatgacgc gaatggcgct
      601 catgacgttt cccttgttgc ggtcccgtat cctgccccgt cattggtcgc ctgttgcgac
      661 gacacagcgg atgagcaggc cactgagaac aaagagctac tgttcctgga tcgtaggttc
      721 acccactgtg gcgacgctca gaaccaggac aagagccagc atatgcggtt tggtgccgga
      781 cggaaacacc gcctcggtcg cggcggaccg cttccgagcg cacacgcgtc tgatatccgt
      841 gggtgcgcat ccgtgggttc gcacccgcac ggcgcatatg ctttcgccat gacgtgctcg
      901 gccataatag cgtgaaaacg gttccggaag gtccggccgt caaccttttc ttcatttttt
      961 gccggcatac cactgtccgt gcgcacgctg gccaaccacc ccaatacgat cgtcctcagc
     1021 ttcgcgacaa cgctgttggg gggactcctc gccgtcgggc tgatcgtccc gccgctgttc
     1081 gggatgctgt tggcggcggc cgtcgccgtc ggcatctgtt tcctggcgtt ctgtttcccc
     1141 acgatcgtct gcgtcgtctg gctgctggtc accagcctga gcctcgagat ggcgatgcac
     1201 gacctggtcg ccgccgaaag cttccaaccg accatcgccg tcgtgaaagg aaccgaaatc
     1261 ggcctggcct tgctttgcat gatccggttc ggccccaagc cggatccgct gtctccggcg
     1321 tggggcttcc tggtcattct ggcagcaggg cttgttcatg gtctgtatcc aggcctgagc
     1381 atggcagaca gcttgcggtc gatgatcggc tccgtcgcac cgttcgtctt ttgcttctgc
     1441 cgcgtgccgc gttcctgggc ggacgcgatg atccaggcca caaaatggtg tcccgtggtt
     1501 gtcgtcctgg caggcctgcc gctggccgtc gttggcatca gacccctgtt catcgatagc
     1561 ggcggcgccc gcctcgccgg tctcggccat cccgccttcc tggctgggat ctgtctgcca
     1621 gcgatctatg cctgcctgat cgagctttat cgggaagggc ggcggggcga cctgctattg
     1681 ctggtgatca acttccttat cctggccctg accggcgccc gcgcaccgtt cgtctgtgcc
     1741 gccgcggtga ccggaatcag cctgatctcc atccgctccg ttgttttcac cgcgcgggac
     1801 cggctgttgc tggtcctctc tgcactggcg gtaatgtcgg tgctggcgat attcgcgggc
     1861 gacctcgcgg aactccgatt gttcaacgtc ttcttgcacg agacgagcaa cctcagcggg
     1921 cgcggattgc tatggcccag cttcgaggac gccgcggcgc aatcgccgtg gttcggctgg
     1981 ggtagcggcg ccggcaacgt cgtcatttca cccgatggcc gaatcgcccg cgttctgcac
     2041 acctgggcgg cgcacaacga gtatctgcgc attggcgtcg aaggcggcct ggttggcgaa
     2101 atcctgctga tcgccctgtt cgccgggtgg gcgctggtcc atacccaccg gctacaggct
     2161 tcggaccggc ggatcatgcg gctggcgttc ctcgttcttg ccggccatgc ggtgaccgac
     2221 aacgtgctga tctcgacccc cgcctgcgtg atgttcgcct tcgccaccgc ggtcttcgct
     2281 cgtaaagcac ccgaatagcc ttgcccgatt ccgggcttct ggcgtaggga acgaagcttc
     2341 ctgccggagt atcgataggt catgcggcgc ttttttctgt tgcttttctg tgctttgtcc
     2401 tgcgctgccg ccactgcggc aaaggcgggc ggcatcgcct tcgtggtcaa ctccggaagc
     2461 gccacgatca gcgtgatcga catgacgacg ctaaaggaaa tccagcgcat ccccacgctg
     2521 cgggagccgc atcacctggt ccttagcccg gacggacgca gcctgctggt cggcgatacc
     2581 gctggcaatc agatgatgtt cctcgatccc aacaccgggg cggtccagca gcgcatgccg
     2641 gtgaccgatc cttaccagat gggattcagc ccagatggaa agttcctggt cgtgaacggc
     2701 ctgctgcgca accaggttga cgtttacgac gccgtcacgc tcaagctgct caagcgtttc
     2761 gctgtggttt cgaccccgag tcatttggcc tattcgccgg actcgtcgtc ggtattcgtc
     2821 agtttgcagg atagcgacaa gctggccgcg ttcgacctgc gttcgctgac cgaaaaatgg
     2881 acggttccgg ttggcaggac ccccgcgggc gtgatgtggc tgaacggcaa ggtgctggtc
     2941 gcggatatgg gcacggatta cgtggccgtg gtcgatccgg ccagcggcaa ggtgctggag
     3001 catgtgcaaa ccggcaaagg cgcgcacaat ctgttcctgt cacccgatca caagatccta
     3061 tgggtgaaca accgagcggg aagctcgacg atgtcgctgg atgcgtccac gttgaagccg
     3121 atccgttcgt atgcgattcc cggcggcccg gacgatatgg atttcgcacc gggcgggaag
     3181 atctggatca cccgtcgctg ggcggagaag gtcgcgatcc ttgatccggc caccgggtcg
     3241 tatgaaacga tcaacgtcgg ccgctcgccg catgggctgt tcctgaatcc gaaggcaccg
     3301 tcgccggcgg tgctgtccag ccgctgatgg acgatccgtt cgatctcgct gccggatggc
     3361 tgcaggagca cctgctgatc ccggccctgt atcagttcgg gttgatgaac tgggaggacg
     3421 tgtcgtacgg ctgggcgctg ttcgcgttgt atggcgtggc gcaagtggcg gtgacgttgg
     3481 cggtttgcct gccgctggag cgttggcgcc cggtggagcg ctggcccgac cacagcgccg
     3541 tctgggtcga tgtcgcctat acgcttttgt cgcgcgtcgg cgtcctgccg ctggtcactt
     3601 acgtgatgtt ctatcgcgcc caggtcacgg tcagcgggtg gatgacggac cgaggcattg
     3661 tgccaccaac cctggagggc ctgttcccga tcctgctcgg tcaacaggta ctgacgtttg
     3721 tgctttatgc gatcattctg gatttcgccg actactggcg ccaccgtctg tcgcatatgt
     3781 ttcgctggtg gtggtcgttg cactcgctgc atcatgcgca gcggcagatg acgttctggt
     3841 cggacgaccg caaccatgta ctggatgacc tgatcgcatt tctctggttt ggcgcggtcg
     3901 cgctgctgat cggggtgccg ccgctgcagt ttcccctgct ggtgctggtg ctgcggttcc
     3961 tggagtcgct gtcgcacgcc aatgttcggc tgtcgttcgg gtggatcggg gaacggcttc
     4021 tggtatcgcc gcgatttcat cgcgcacatc atggcgtgct ggccgtggca caacgcagtt
     4081 gcaactacgg cgcggtgctg ccgtggtggg atatactgtt ccggacggcc gattttagcc
     4141 ttggatacgc ccgaacgggc gacccttcgg gcgaggaggc gctggcgacg gggacatgga
     4201 tcgagcagca gaaagcggga ttgcggcggc tcgcccggag tttcggtcaa cggcgggcga
     4261 ggcgtggaac atgagcaaat agaagcctgt ttaacctgtc gtcaaccgcg gatcatggga
     4321 ttgttgctgc agcgcagtgg agtgtgtggt aggcgacgaa cctgccccct gatccaggtt
     4381 tacctggtcg aggtgcggct cgaccgactt gaacggcgct tggaaatcgt gcacgcctga
     4441 ttcttcggcc ggctccgcca ttgcgtgtag gcggcgtaat ctgcttcgac cggatccctt
     4501 catcgaccag atcccttcat cgaccggatc ccttcatcg
//
LOCUS       DASWQN010001038         3908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1683635, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001038 DASWQN010000000
VERSION     DASWQN010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683635"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..639)
                     /locus_tag="VGC82_17745"
     CDS             complement(115..639)
                     /locus_tag="VGC82_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425166.1"
                     /translation="MALIVASGICLTPLFACAAPFAALATLAALKLGRRDMIAVMVAV
                     WSANQAIGYGMLGYPWSWDSAAWGIAIGMSCGLAVLAAKGLSTTGRAPLAVSLPFVGA
                     FATFELGLYVAGFMLPGSDEAFSFAVIEHIFLINVSALFGLVVIYHSTMLVGRLAGTG
                     AALPHGLGATTSGR"
     gene            complement(776..1552)
                     /locus_tag="VGC82_17750"
     CDS             complement(776..1552)
                     /locus_tag="VGC82_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0425167.1"
                     /translation="MAYQFCTVQDEGRLRIVTLNRPEVLNALHADANDELAAVWDEFA
                     ERDDLWVGIVTGAGERGFSAGNDLKVQAGGKRRPNGPRGFAGICNRFDLSKPLIAAVN
                     GVAMGGGFETALACDIIIAAENAVFALPEPRVGLIAGAGGVHRLPRSIPIKKAMGMIL
                     TGRRVPAREGFELGFVTEVVPEGQSVEAAKRWAAMILECSPKAVRASKQASYRGMDEP
                     TLEQAMRTVYPAQQENLGSQDYVEGPKAFAEKRKPNWQNR"
     gene            complement(1552..2772)
                     /locus_tag="VGC82_17755"
     CDS             complement(1552..2772)
                     /locus_tag="VGC82_17755"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0425168.1"
                     /translation="MSGENCLAGLKILDLTQFEAGPSCTEALAWMGADVVKIENPKVG
                     DPGRSSGGKAAHDALYFLQYNANKRSVAVNLKDPRGLELVKELAKTADVMIENFAPGA
                     IERLGLGYDVVRAINPAIIFCQVKGFGTGSPFEKNLAFDMIAQACGGLMSITGEEDGP
                     PCKPGATIGDTGTGMLMAVSILGAYVRRLRTGQGEHLQVAMQDAILHYIRNAFAFMER
                     TDGKAAPRAGSKTVGGGNPPIGVYPCKGGGPNDYVYIYTSAANPEHWTRLLKVMGRED
                     LMGDARFSTPAARTENREEVDRIVAEWTIRHDKHTAMQLVGGATIPAGAVLDTRELAD
                     DPTFRERGIRQAMRHPEVGEYVMSGWPVRFGGHTPAITPAPLLGQHGQEVLSQWLNYD
                     AARIAELKNSEVIA"
     gene            2905..3189
                     /locus_tag="VGC82_17760"
     CDS             2905..3189
                     /locus_tag="VGC82_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425169.1"
                     /translation="MVEPVNIASYRDDTMWAVVASIGRTSRVAEVFSSRLAALADLAW
                     REQQVTAYANFLRNSRQPTPSYTVKPIRRTDLPRSWRPLPALGFLHGKLM"
     gene            complement(3316..>3908)
                     /locus_tag="VGC82_17765"
     CDS             complement(3316..>3908)
                     /locus_tag="VGC82_17765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0425170.1"
                     /translation="QRGAKLIAQLMAFARKQNLQVEYLPLNTLVEEMQELLGRSVGAA
                     VRLIYDFQPDVWPVMADASQVQTALLNLSINARDAMPGGGDLRIATGNVHVPVAEADL
                     AAGDYAALTVQDNGSGMTDEVKSRLFEPFFTTKGVGKGTGLGLAQVHGFVHQSGGTVR
                     VHSVLGEGTKFTILLPRAVAGGGEFAGGGATEQVAP"
BASE COUNT          695 a         1295 c         1184 g          734 t
ORIGIN      
        1 cgatgacgcc acgctgcacg atcagcgtcg ctcgatctga aagagagtgt tcgacgacag
       61 gatcacgcaa ctcattccgc taatgaaccc gaggctaaaa cgcccgcgtc cctgctaccg
      121 gcccgaagtg gtggcgccaa ggccatgcgg caacgccgcg ccggttcctg ccaatcgtcc
      181 gaccagcatc gttgaatgat aaatcaccac gagaccgaaa agtgccgata cgttgattag
      241 aaatatatgc tcgatcaccg cgaaactgaa cgcctcgtcg cttcccggca gcatgaagcc
      301 ggcaacgtaa agccccaatt cgaacgtcgc gaacgcgccg acgaacggca ggctgacggc
      361 caacggtgct cgaccggtcg tggacaaacc ctttgcggcg agaacggcca acccgcacga
      421 catcccgatt gcgattcccc acgccgcgct atcccaggac cagggatatc cgagcattcc
      481 ataaccaatc gcctggttcg ccgaccacac ggcgaccatg acggcaatca tgtcacgccg
      541 gccgagtttc aacgcggcca gagtagccaa ggcggcgaac ggtgcggcgc aggcgaacag
      601 cggcgtcagg cagatcccgc tggcaacgat cagtgccatc caaagcgcct gccccgattg
      661 ccgcgcttca agacgatcgc tgcgtctgtc cggaattgat ctcattgctt ctgcccgcag
      721 ggtggaaagc aaaactaagc cttaacatcc cggcgtcaac cgtttgatcg ggacgtcatc
      781 ggttctgcca gttgggcttg cgtttttcgg cgaaggcttt cggaccttcg acataatcct
      841 ggctgccaag attttcctgc tgcgcgggat aaaccgtgcg catcgcctgt tcgagcgtgg
      901 gttcgtccat gccgcgatag gacgcctgct tcgaggcgcg aaccgccttg ggcgagcatt
      961 ccaggatcat cgccgcccat cgcttggcgg cttcgacgga ctggccttcc ggtaccactt
     1021 cggtgacgaa gccaagttcg aagccctccc gcgccggcac ccttcggccg gtcaggatca
     1081 tacccatggc cttcttgatg ggtatcgagc gtggcaggcg atgcaccccg cctgcccctg
     1141 cgatcagccc gacacgcggt tccggcagcg caaagacggc gttttccgcg gcaatgatga
     1201 tgtcgcaggc cagcgcggtc tcgaagccgc cgcccatcgc gactccgttc accgcggcga
     1261 tcagcggctt gctgaggtcg aagcggttgc agatcccggc gaaaccgcgg ggaccgttcg
     1321 gacgccgctt gccgccggcc tgaaccttga ggtcgttgcc ggcggagaac cctcgctccc
     1381 ctgccccggt gacgatgccg acccacagat cgtcgcgttc ggcgaactcg tcccacaccg
     1441 cggccagttc atcgttggcg tcggcatgga gcgcgttcag cacctcgggc cggttgaggg
     1501 tgacgatccg aagccggcct tcgtcctgga cggtgcagaa ctggtatgcc atcaggcgat
     1561 gacctcgcta ttcttcagct cggcgatgcg ggcggcatcg tagttcagcc actgcgaaag
     1621 aacctcctgg ccgtgctggc cgagcagcgg cgcgggcgtt atcgctggtg tgtggccgcc
     1681 gaaacggacc ggccagccgc tcatgacata ctcgcccacc tccgggtgcc gcatcgcctg
     1741 gcggatgccg cgttcgcgga acgtcgggtc gtcggcgagt tcccgcgtgt ccagcacggc
     1801 gccggcgggg atggtggcgc cgccgaccag ttgcatcgcc gtgtgcttgt cgtgccggat
     1861 cgtccattcc gcaacgatcc ggtcaacttc ctcgcggttc tcagtgcggg cggccggggt
     1921 ggagaagcgc gcatcgccca tcagatcctc gcgccccatg actttcagca gtcgggtcca
     1981 gtgttcggga ttggcggcgc tggtgtagat atagacgtag tcgttcggtc cgccgccctt
     2041 gcacgggtac accccgatcg gcggattgcc accgccaacc gtcttcgatc cggcacgcgg
     2101 cgccgccttg ccgtcggtcc gctccatgaa cgcgaaggcg ttgcggatgt agtgcaggat
     2161 ggcatcctgc atcgcgacct ggaggtgctc accctggccg gtccgcaggc gccggacata
     2221 cgcgcccagg atcgacaccg ccatcagcat cccggtcccg gtgtcgccga tggtcgcacc
     2281 cggcttgcag ggcggcccgt cttcctcacc ggtgatggac atcaggccgc cacaggcctg
     2341 ggcgatcatg tcgaacgcga ggttcttctc gaacgggctg ccggtgccga agcccttgac
     2401 ctgacagaag atgatcgccg gattgatcgc ccgaacgacg tcatagccga gcccaaggcg
     2461 ctcgatcgcg ccgggggcga aattctcgat catcacgtcg gcggtctttg ccagttcctt
     2521 gaccagttcc aagccgcgcg gatccttcag gttgaccgcc acggaacgct tgttggcgtt
     2581 gtactgcaga aaatacagcg catcgtgcgc ggccttgccg cctgacgagc ggccggggtc
     2641 gccgaccttc ggattctcga tcttgacgac gtccgcgccc atccaggcga gggcttcggt
     2701 gcaggatgga ccggcttcaa actgggttaa atccaggatt ttcagcccgg ccaggcaatt
     2761 ctctccgctc atcgtggcat ttcccgttgg ttttgtagaa atgctacgct ggacaaggga
     2821 ccgccgtcga gagcggccac ggatttcgtt gaattggttg aaagcctctt gccttccggc
     2881 gcgcgggaag ccagccttta gagcatggtg gaacccgtga acattgccag ttaccgtgac
     2941 gatacgatgt gggccgtggt cgcatcgatc ggccgaacat cccgggtggc ggaagtgttc
     3001 tccagccgcc tcgcggcgct ggcggacttg gcttggcgcg aacagcaagt gaccgcgtac
     3061 gccaacttcc tgcgaaacag ccgccagccg acgccatcct ataccgtgaa gcccatccgc
     3121 cgaaccgacc tgcctcgatc gtggcggcca ttgccggcgc tcggcttcct gcatggcaag
     3181 ctgatgtagc gaggctggat caaagcattt tcagcctgac tgtgcaggtc gaaaatgttc
     3241 tgcctccttg ttttggtcgt atgatccaca cgcttcggac gttccgtcgg gcgtgatcac
     3301 cctccagtgc cggcgctatg gcgccacctg ttcggttgca ccgccgcctg cgaactcgcc
     3361 acctcccgcg acggcgcgcg gcagcaggat cgtaaatttc gtcccttcgc ccagcacgct
     3421 gtgcacccgc acggttccgc ccgattgatg gacgaagcca tggacctgcg ccaatcccag
     3481 gccggtgcct tttccgacgc ccttggtggt gaagaacggc tcaaacaacc gggattttac
     3541 ttcatccgtc atgccggagc cgttgtcctg gaccgtcaac gccgcataat ccccggcggc
     3601 cagatcggct tcggcgaccg gaacgtggac attgccggtg gcgatccgca agtctccacc
     3661 gcccggcatg gcatcccggg cgttgatcga caggttcagc aacgccgtct gcacctgact
     3721 ggcgtcggcc atgaccggcc acacatccgg ttgaaaatca tagattaaac gaacggctgc
     3781 cccgacgctg cgccccagca attcctgcat ttcctcgacc agcgtgttca gcggcaggta
     3841 ttcgacctgc aggttctgct tgcgggcgaa cgccatgagt tgcgcgatca gtttcgcacc
     3901 gcgctggg
//
LOCUS       DASWQN010001039         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2474856, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001039 DASWQN010000000
VERSION     DASWQN010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474856"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1216
                     /locus_tag="VGC82_17770"
     CDS             185..1216
                     /locus_tag="VGC82_17770"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0425171.1"
                     /translation="MNRRALLAGCGALAAGIPFRVRAAGVTPELIDAARKEGELTWYI
                     AQVDTETAEGMGRAFTAQFPGVRVSVIRTTGQVAYERLLQDLKNNAPQCDVYSSTDIS
                     QYVALRKRDALANYVPAGAATLLPAFQKVADPGYTYPSAATSHLLIYNSARVKPEEAP
                     KSWPDMLDPRWKGRVAVGHPAFSGCTGIWALALTKAHGWEFFEKLAKNNPRIGRSGND
                     PVTLINAGECLLGPAPANTAFQQVDKGNPIVPVYPADGATLCVGPSSVMANAPHPNAA
                     RLYMEWLMSDAFSKLSVANHGDPVHPGVALTSGQKPLDQVPLLALSVQEIAKGIPEIV
                     EKWRDTFGG"
BASE COUNT          349 a          561 c          543 g          303 t
ORIGIN      
        1 accgatcatg ccggcggacg tatcggcgaa aaccaagaag taccaggctc gttggagcat
       61 gacacaggca agtccgggct cgtcggagca tgagacaggc aagtcggcca taagcaacac
      121 caagctttgg tggttgccgg tcccggacgg tcaggcggag tgaaaaggat acaggatgca
      181 cctgatgaac cgacgcgctt tactggctgg ttgcggcgct ctggctgccg gtatcccgtt
      241 tcgggtccgc gccgccggcg tcacccccga actgatcgac gcggcgcgca aagaggggga
      301 gctgacctgg tatatcgccc aggttgacac cgaaacggcg gaaggcatgg gacgggcatt
      361 caccgcccag tttcccggcg tgcgggtgtc ggtgatccgc accacgggac aagtcgcgta
      421 tgagcgcctg ctgcaggacc taaagaacaa cgcgccgcaa tgcgacgttt atagttccac
      481 cgatatctcg cagtatgtgg cgctgcgcaa gcgcgacgct ttggcaaact acgtccctgc
      541 cggagccgct accctgctgc cggcgttcca gaaggtcgcc gatcccggct acacctatcc
      601 atccgcggcg acctcgcatc tgctgatcta caacagcgcc agggtcaagc cggaggaggc
      661 gcccaagagc tggcccgaca tgttggaccc gcgatggaag ggacgcgtcg ccgtcggcca
      721 tcccgccttc agcggctgca ccggcatctg ggcgctggca ctgaccaagg cccatggctg
      781 ggaattcttc gaaaaactcg cgaagaacaa tccccgcatc gggcgctccg gcaacgatcc
      841 cgtcaccctg atcaacgccg gcgagtgtct gctgggtccg gccccggcga acaccgcgtt
      901 ccagcaggtg gacaagggca accccatcgt cccggtctat cccgccgacg gcgccacgct
      961 atgcgtcggc ccatcgtcgg tgatggccaa tgccccgcat cccaacgccg ctcggctcta
     1021 catggagtgg ctcatgagcg acgcgttttc caagctgtcc gtcgccaatc acggcgaccc
     1081 ggtgcatccg ggcgtggcgc tcacctcggg gcaaaagccg ttggatcagg tgccgctgct
     1141 ggcactgtcg gtgcaggaga tcgccaaggg gatcccggag atcgtggaga agtggcggga
     1201 cacgttcggc ggataacaat tgaccagcga cacgtttatc acggcctcgg cctctcgagg
     1261 tgatgcccgg gaaccgtggc cggtgcgggc ggtgctggaa acgccgcttt ccgagtggct
     1321 taaagcgcgc gtcgggccgg cggcgaccaa cgtgtgcgtt cactccttct tactttcatc
     1381 acggaggaag aacgagtgcc acgaaggagg atttttgtta atccatcagt ctgcttagcg
     1441 gctaatgaca aataatcgct tcgaacgagg catgcctccc ggcaaattgt ggcagacgaa
     1501 ggtctggcac ccaaggcttg cgatgcgcga cctactcaag acgcctatgc cgctctggcc
     1561 caccaagaca gatctactgg cgggctcgct ccgaccgcgt tatctgctgg ccgacacata
     1621 ccggcaatgg gtaatcgggc ttctctccgt ggcgctccgc ggccctcgcg cttctccgtg
     1681 ttgaaaatcc ctggcccaac aagcgcgcga tagtcgaacg agggccatcc tctcgcaaaa
     1741 caagacgtgc agccaa
//
LOCUS       DASWQN010001040         3171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_365028, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001040 DASWQN010000000
VERSION     DASWQN010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365028"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..1661)
                     /locus_tag="VGC82_17775"
     CDS             complement(315..1661)
                     /locus_tag="VGC82_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0425172.1"
                     /translation="MRRIDELAQALADGTTTSRALIEDSLARIADPDGEGKRTFIKVY
                     ADQARAMADAADLLRRNGRAPGPYAGIPIALKDLFDIAGEPTPAGSAILAEAPDAIAN
                     APAVARMLAAGFIPVGRVNMTEFAFSGLGINPHYGTPTSPWDRSSRRIPGGSSSGTAV
                     AVADGMAAAGLGTDTGGSCRIPAAFCGIVGYKPTARRVPIAGVLPLAPSLDSVGPLAS
                     SVACCAAVDAILAGEIPAAPVPANLDGLRLAVPQNTALDGMDQTVAAAFDKALSSLSA
                     AGARIRTVHFTEFSDLAAVNAKGGFAASEAYAWHRHLLATRGSGYDPRIRVRIARGEH
                     MSAADYLDVRSARTRLIASFDRATAPFDCVLMPSVPIVAPRIADLDDERAYNSINMLI
                     LRNTALGNFFDRCSISLPCHRLGEAPVGLMLTGETMGDARLFAIASAVEAVLRSDE"
     gene            1694..2536
                     /locus_tag="VGC82_17780"
     CDS             1694..2536
                     /locus_tag="VGC82_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0425173.1"
                     /translation="MTDTPAKPVLLTGASGALGRVLVRSLGALGWRLVLTDIAPFPDA
                     LPAGAVFTKADLSDGMTILRLAEGCGTIIHLGGVSVERPFEEVIGPNIRGLYHIYEAA
                     RREKARVIFASSNHSVGFHERTESLPADTQFLPDGYYGLSKAYGELMGRMYWFKHGVE
                     SVNVRIGSACPEPVNARMLASWMSYSDFSRLMERCVLASKVDHAVIWGASKNARMTWW
                     RDDARDALGWTPTDSADPFAGQLAGAVSGDPVEERYMGGAYCSIDYTREEPAPSGVFG
                     GNGD"
     gene            2529..>3171
                     /locus_tag="VGC82_17785"
     CDS             2529..>3171
                     /locus_tag="VGC82_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0425174.1"
                     /translation="MTDGILYIGNKRYSSWSLRGWLAVKLAALDVDVQVLRFTRPGTT
                     KAIAELSPNGLVPFLDHNGVRVWESLAICDYCAELQPSLWPADQVARAHARSISAEMH
                     AGFRDLRMAMWMNLGRDFAGLGRTAGALADIARIEAIWADTRSRFGAGRPFLFGSEFG
                     AADAMFAPVVARFLGWRPDISAEASSYCAAVRAHKLVGEWYEAAMAEPAEWLID"
BASE COUNT          584 a          938 c         1078 g          571 t
ORIGIN      
        1 ggtgtgcata ttcgatttct gcttcaatcc cagcagaatg tcgccgctga cctcaatctt
       61 gggcatttgc cggtgatacg gcgatgcatc acccggcagg tgtcgcaacc tgagacaatt
      121 cagccggatt gccctgccca gttgcccgag gttcgccagc atcgcccagc acacggctta
      181 tcgccggaga acgacggatg cctcccggtt tcgtcgcttg cagcatgcaa tcgctcgaat
      241 ttagggtgcg agcgtctcgt gtttagggtg cgagcgtctc gcggaacaac gccctttgca
      301 cgacttcaag ctggtcattc atccgaccgt agcaccgcct ccacagcgga ggcgatcgcg
      361 aacaaccgag cgtcgcccat cgtttcgcct gtcagcatca aaccgaccgg agcctcgccg
      421 agacgatggc aaggcaatga gatcgagcac cggtcgaaga aatttcccag cgccgtgttg
      481 cgcaggatca gcatgttgat gctgttgtag gcacgctcgt catcaagatc ggcaatgcgg
      541 ggcgcgacga tcggcacgct cggcatcaag acgcagtcga atggggcggt ggcgcgatcg
      601 aagctggcaa tcaggcgggt acgcgcactc cgcacatcca gataatcagc agcactcata
      661 tgctcgccgc gagcgatgcg gacgcggatg cgggggtcat agccactgcc ccgggtcgcc
      721 agcaaatggc ggtgccaggc ataggcttcc gatgcggcga acccgccttt ggcattgacc
      781 gcggcaagat cggagaattc ggtgaaatgg accgtccgga tgcgcgcgcc cgccgcggac
      841 aggctggaca gcgccttgtc gaaggcggcg gcaacggtct gatccatccc gtccagagcc
      901 gtgttctgcg gcacagcgag gcgcagacca tccagattgg ccgggacagg agccgccggg
      961 atttcgccag ccaggatggc atcgaccgcg gcacagcaag cgacactaga agccaacggg
     1021 ccgacggagt ccaacgacgg cgccaacggt agcacgccgg cgatgggaac ccgcctggcg
     1081 gttggcttat agccgacgat gccacagaac gcagccggaa tgcggcatga gccgccggtg
     1141 tcggtgccca atccggcggc tgccatcccg tcagccacgg ccaccgccgt gccggacgaa
     1201 ctgccgccgg gaatgcgtct ggaggagcgg tcccaagggc tggtgggagt gccgtaatgg
     1261 gggttgatgc ccaggccgga gaaggcgaac tcggtcatat tcacccgccc caccggaatg
     1321 aagccggcag ccagcatacg ggccaccgcg ggagcgttgg cgattgcatc gggcgcttcg
     1381 gcaagaatgg cggaaccggc tggcgtgggc tcacccgcga tgtcgaacag gtctttcagg
     1441 gcgatcggaa tgccggcata gggaccgggc gcccggccat tgcggcgcaa caggtcggcg
     1501 gcatcggcca tggcgcgggc ctgatcggca taaactttga tgaaggttcg tttaccctcg
     1561 ccgtcaggat cggcgatcct ggccagcgaa tcctcgatca gggccctgct ggtcgtcgtt
     1621 ccatcggcca gggcctgggc cagttcgtca atcctgcgca tgatacctcc gctacgaata
     1681 acaggagcat gccatgactg acacgcctgc caaaccggtc ctgctgacag gagcttccgg
     1741 cgcactggga cgcgtcctgg tgcgatcgtt aggggcactg ggctggcggc tggtgctgac
     1801 cgatatcgcg ccgtttccgg acgcgctgcc cgcgggggcg gtgttcacca aagcggatct
     1861 gtcggacggc atgacgatac tgcgcctcgc tgaaggctgc ggcacgatca tccacctcgg
     1921 cggcgtgtcg gtggagcggc cgttcgagga ggtcatcggg ccaaatatcc gcggcctgta
     1981 tcacatctat gaagcggcaa gacgggaaaa ggcgcgggtg attttcgcgt cttcgaacca
     2041 ttcggtgggg tttcacgaac ggaccgagag cctgccggcg gacacccagt tcctgccgga
     2101 cggatactac gggctgtcga aagcctatgg cgagctgatg ggccggatgt actggttcaa
     2161 gcacggtgtg gaaagcgtca acgtccgtat tgggtcggcc tgtccggagc cggtaaatgc
     2221 gcgcatgttg gcatcctgga tgtcgtacag tgatttctcg cggctgatgg agcgctgcgt
     2281 gctggcatcc aaagtagatc atgcggtcat ctggggcgcc tcgaaaaatg cgcggatgac
     2341 atggtggcgc gatgacgctc gggatgccct gggctggacc cccaccgaca gcgccgaccc
     2401 tttcgcaggg caattggccg gtgccgtaag cggcgacccg gtggaggaac gttacatggg
     2461 cggggcatat tgttcgatcg attacacccg tgaggaaccg gcgccttccg gggtgttcgg
     2521 aggcaacggt gactgacggg atcctgtata tcggcaacaa acgctactcg tcgtggtcgc
     2581 tgcgaggctg gctggcggtg aaactggccg ctcttgacgt ggacgtgcag gtgctgcggt
     2641 tcacccgccc cggtacgaca aaggcgatcg ccgaactgtc gcccaatggg ctggtgccgt
     2701 tccttgatca taatggcgtc cgcgtatggg aatcgctggc gatctgcgat tactgcgcgg
     2761 aattgcagcc gtcgttgtgg ccggccgacc aggttgcccg agcgcatgcg cggtcgatct
     2821 cagccgaaat gcacgccggc tttcgcgact tgcgcatggc gatgtggatg aacttggggc
     2881 gcgacttcgc gggcttggga cggactgcgg gagccttggc cgacatcgcc aggatagagg
     2941 cgatttgggc ggacacgcga tccaggttcg gggccggccg gccgtttctg tttggcagtg
     3001 agttcggagc agccgatgcg atgttcgcgc cggtggttgc tcgctttctg ggatggcggc
     3061 cggatatttc cgctgaggca tcctcctatt gcgcggcagt ccgagcacac aaactggtgg
     3121 gcgaatggta tgaagcggcc atggcggagc cggccgaatg gctgatcgac g
//
LOCUS       DASWQN010001041         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_232959, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001041 DASWQN010000000
VERSION     DASWQN010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232959"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VGC82_17790"
     CDS             <1..336
                     /locus_tag="VGC82_17790"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0425175.1"
                     /translation="TAATRSPIGVLRDDPDILPLFARAAAEVAAVGHAKGIRIPEDAA
                     ETVMGFVRSAPPAMMASMAHDLIRGNRIELPWLSGKVVALGKELGVATPVHEILYAVL
                     KPFVAGTPT"
     gene            complement(372..1280)
                     /locus_tag="VGC82_17795"
     CDS             complement(372..1280)
                     /locus_tag="VGC82_17795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0425176.1"
                     /translation="MLKSAVRLLFRRTRPQPQVLPEPPSRAGQAAARTVAPVEVLPRT
                     AWPASRLALANQLWGQGFIIPGGEIETLRLARPLGLSAASSLLMVGAGSGGPATSVAR
                     NLGAWVTAMDNDASLLAAARGLTNRLQMGKKVTIKAWDPENPVFPAKSHHHCLALEPL
                     HGSRPEPILDGLARALRPGGQLVLTELTATTPLNPADPAVSRWSVLEKRDPANMVSGV
                     SVTRMLGRVGLDVRISEDISDRHMEHAMLGWRIMLRDLRDQKPTPHEALQLVAEAELW
                     LLRRRLIRDGRLRMMRWQAISRVPIV"
     gene            complement(1423..1758)
                     /locus_tag="VGC82_17800"
     CDS             complement(1423..1758)
                     /locus_tag="VGC82_17800"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0425177.1"
                     /translation="MTNSPYLFRAAEHPLREATDRLKVREAVSGFGIALRYQERAPDP
                     PGTPAGNWCETGHSIFILSGRISYQFDTDTVEAGPGDMLHIPAGPQHRHRPRVVGSEV
                     VRYVLTEFD"
BASE COUNT          309 a          597 c          638 g          321 t
ORIGIN      
        1 acggccgcga cacgctcccc aatcggcgtc ctccgcgacg acccggatat tttgccgctg
       61 ttcgctcgcg ccgcggccga ggtggccgcg gtcggccacg ccaagggcat caggattccc
      121 gaggatgcgg cggaaacggt catgggcttc gtccgcagtg ctccgccggc gatgatggcc
      181 tccatggcgc acgacctgat ccgcggcaac cgcatcgagc tgccctggct gtcgggaaaa
      241 gtggtcgcac tgggcaagga gctaggggtt gctactccgg tgcatgagat cctttacgcg
      301 gtgctcaaac cgttcgtggc gggcacgcca acctgatcgt cagacgacct gagcatcaga
      361 cgagctgatc gtcagacgat cgggacgcgg ctgatcgcct gccagcgcat catccgtagc
      421 cgaccgtcgc ggatcagccg ccgtcgcagc agccaaagct cagcttccgc caccagttgc
      481 agcgcctcgt gcggcgtcgg tttctgatcg cgcaggtcgc gcagcataat ccgccaaccg
      541 agcatcgcgt gttccatgtg acggtcggag atgtcctcgc tgatgcggac gtccagcccg
      601 acccggccca gcatgcgcgt aacggagact cctgacacca tgttggccgg gtcgcgcttt
      661 tccaggacgc tccagcggct gaccgccggg tcggctgggt tgagcggcgt cgtagccgtc
      721 agttcggtga gaaccagttg gccgccgggg cgcaatgccc gcgccagccc gtccaggata
      781 ggttcgggcc gggagccgtg aagcggttcg agcgccaggc agtgatgatg gcttttggcc
      841 gggaagaccg gattttccgg gtcccaggcc ttgatggtga ctttcttgcc catctgcaaa
      901 cggttggtca gccccctcgc ggcggcgagg agcgacgcat cgttgtccat agcggtcacc
      961 caggcaccca ggttgcgcgc gacggaggtg gccggaccgc cgctgccggc gcctaccatc
     1021 agcaggctcg atgccgcgga caacccgagc gggcgggcca ggcgcagggt ttcgatctca
     1081 ccgccaggaa tgatgaagcc ttgtccccag agctgattgg ccagtgccag ccgggaggcc
     1141 ggccatgctg tccgtggcag gacttcgacc ggcgcaacgg ttcgagcggc tgcctgcccg
     1201 gcgcggcttg gcggctccgg cagcacctgg ggttgcggcc gcgttcgtcg gaacaacaag
     1261 cgcaccgcgg atttcagcat gtatcgatct ccatcgaccg atactaagga gtcttcctta
     1321 ccgaaacgtt aatccgcggc acgctgcgca tcccggggcg tgcggatggg tttgcctatt
     1381 gcacggccac ggcctcggcg tcgcgcccaa cgctgtcacg ctttagtcga actcggtcag
     1441 cacgtatcgg acgacctcgg agccgaccac gcggggccgg tggcgatgct ggggaccggc
     1501 ggggatgtgc agcatgtcgc caggtccggc ttcgaccgtg tccgtgtcga actggtagct
     1561 gatgcggccg gacaggatga agatggaatg gcctgtttcg caccagttcc ctgccggcgt
     1621 gccgggggga tcaggcgcgc gttcctggta acgcagggcg ataccgaacc ccgataccgc
     1681 ttcccgcacc ttcagccggt cggtcgcctc gcgcagcggg tgttcggcgg ctcggaacag
     1741 gtagggactg ttggtcatgg cgtgagatta tacggttcgg tggtggggcg acatagacgt
     1801 gggtgaccgg gccaagcccg gtcatgacac gaaagaaaag ccgggtcgtg acaccaaagc
     1861 caagc
//
LOCUS       DASWQN010001042         5486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2475067, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001042 DASWQN010000000
VERSION     DASWQN010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2475067"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(501..1898)
                     /locus_tag="VGC82_17805"
     CDS             complement(501..1898)
                     /locus_tag="VGC82_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0425178.1"
                     /translation="MAHEILIVDDEPDIRLLIEGILRDEGYETRGAGDAESAIAAFRV
                     RRPSLVILDVWLQGSRMDGIAILETLHSEEPHVPVVMISGHGTIEVAVGAIQLGAYDF
                     IEKPFQSDRLLLVVRRALEAAALARENAELRLRAGPEATLTGDSALIVQLRSQVERVA
                     PTGSRVMISGPAGSGKEVVARMIHARSRRSTGPFVVMNCATLNPGRFEEELFGLEAGS
                     DPAAHPRRAGVLERAHGGTLLLDEVSDMPLETQGKIVRALQDQTFERLGGSARVKVDV
                     RVLSTTNRDLQAEIAAGRFREDLYYRLAVVPVRLPALRERREDVPTLALHFIERSAES
                     SGILVRALSADTLAALQAYDWPGNVRQLRNLMDWLLIMAPGNPGDPIRADMLPPEIGL
                     RAPALLNIDPTADIMSLPLREARDLFETQYLHAQLLRFGGNISRTAQFVGMERSALHR
                     KLKQLGVNSDERVGG"
     gene            complement(1888..4149)
                     /locus_tag="VGC82_17810"
     CDS             complement(1888..4149)
                     /locus_tag="VGC82_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY0425179.1"
                     /translation="MRDIISEPRSTRAGQLGDIMLGKTVTLVLTGLALMVGLATFIIL
                     ARGSPLGWRPGVGVGLVLANLSVLLLLGAVLAGRLTRVWVERRRGSAGSRLHVRLVFL
                     FSGVAVAPTIVVACFAVAFFHFGIQSWFNEPVRESLAESLQVSRGYLEEHRNNIRAVA
                     LEMSNDLARAGRFLSADPTVFAEVLDTQTTLRGLTEAIIYEPTTGQVLAAAGIFVGLG
                     VEAPSAAATQQAMNGDVAILNAGDGTRVRAVVRLESTPALMLMIGRPIDPTILGYMRR
                     TEQAVSEYQRLDVNRSWLQVAFAWIFAIVALLVLLAAGLIGLIIANQIARPVGTLISA
                     AERVRSGDLAVRVKEPATGDELAGLSRAFNRMTGQLAAQRAELMDAYSQIDERRRFTE
                     TVLSGVSAGVIGLDAQGRIELPNRAADELLGLDLLAATGHPLAEIVPEFAALFNEVAA
                     SPDKARTAEIQTGPLNHRRILLVRIGAELSGGRTDGFVVTFDDITELQSAQRKAAWAD
                     VARRIAHEIKNPLTPIQLSAERLKRRFTKEIQSDPDTFAQCADTIIRHVGDIGRMVDE
                     FSAFARMPQPVIKPEDVGRIAREALVLQKTARPGIEWVTDIAERGPVVPCDRRMLRQA
                     LTNLLQNAADAVAAREGAGHITLTVQDLPDAARLIVGDDGVGLPQEDRARLTEPYVTH
                     KPKGTGLGLAIVKKIMEDHGGALMLEDQPNGPGALASLVLPRAMAVAATSNGQAHGEN
                     GPNGRMTQVSHGA"
     gene            complement(4224..>5486)
                     /gene="ntrC"
                     /locus_tag="VGC82_17815"
     CDS             complement(4224..>5486)
                     /gene="ntrC"
                     /locus_tag="VGC82_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEY0425180.1"
                     /translation="IPRIKKIRPELRVVVMSAQSTLMTAVKATQRGAFEYLPKPFDLR
                     ELLSVVGRALAAPSEPTFSPVEAKDTEERLPLIGRSPAMQEIYRTIARLTTADLTVMI
                     NGESGTGKELVARALHDYGKRRSGSFVAINMAAIPRELIESELFGHERGAFTGATNRS
                     QGRFEQANGGTLFLDEIGDMPPEAQTRLLRVLQEGEFTSVGGRQPIKANVRIIAATHR
                     DLRTAIRQGQFREDLFYRLNVVPIRVPPLRERVEDIAVLARHFLERAREDGLPAKSLD
                     QGAVDRLKQHAWPGNVRELENLMRRLAALCPDEVVGAAVIDAELTDPLPAQGPIGSVQ
                     ESNGPETLSSAVERHIKEFLAAHNDGMGVTDVYDKIIAEVERPLIRLTLAATRGNQIK
                     AASMLGLNRNTLRKKIRDLEIPVVRGLM"
BASE COUNT          963 a         1915 c         1645 g          963 t
ORIGIN      
        1 acggcgaacc caagacgctg caagagatgg atgccggtgt cgcggcggag gcgaagcgcc
       61 ggcatgctcg caatcgatac taacctcgtc gtccgcgacc tgaccgcgga ccatccaacc
      121 cagtcagccc aagcgcggac aataatcgag gaacgaggtt ttcattggca ccaccgtcct
      181 gcttgaaacg gaatgggttc tgcgtagggt ttatggattt ccttccgcgc gattcaatga
      241 cgcggcacgt ggcttggcgg gcctgccgac agtgacactt gaggatccgg tgctcgttgg
      301 caatgcactg gaatggctga caaacggcat ggatttcgcg gacgcaatcc atcttgcgaa
      361 agccagccac tgcgatgggt tcgtgacatt ggaccgccaa ttcgtaagca ccgcgaaaaa
      421 gcttggttta acgaaagtcc ggacgccgcc gttgcacaag actcggtcca aatccaggta
      481 aagccggtaa tagcggtggc ctatccgccg actcgctcgt cggagttaac ccccaactgc
      541 ttcagtttcc gatgcagcgc cgaacgctcc atgccaacga attgcgccgt ccgagaaatg
      601 ttcccgccaa agcgtagtaa ttgcgcatgc agatactgcg tttcgaacaa gtctctcgcc
      661 tcacgcagcg gcaaggacat gatgtcggcg gtcggatcga tgttgagcag ggcgggcgcc
      721 cgcaatccga tctccggcgg cagcatatcg gcccgaatcg gatcaccggg attcccgggc
      781 gccatgatca gaagccagtc catcaaattc cgcagctgcc gcacatttcc cggccaatca
      841 tacgcctgca gcgccgccag cgtgtccgcc gacagcgccc gcaccaaaat cccagaactc
      901 tccgccgagc gctcgatgaa atgcagggcg agcgtgggca cgtcctcccg gcgctcccgc
      961 aaggccggca ggcgaaccgg cacgactgcg aggcggtaat acagatcctc gcggaaccgc
     1021 ccggcggcaa tttcggcttg caggtcgcgg ttggtggtgg acagtacccg cacatccacc
     1081 ttcaccctgg ccgacccgcc cagccgctca aacgtttggt cctgcaatgc tcggacgatt
     1141 ttgccctggg tctccagcgg catgtcggag acctcgtcca gcagcaatgt tccgccatgc
     1201 gcccgttcca gcacacccgc gcgccgcgga tgagcggccg gatcgctgcc cgcctccagc
     1261 ccgaacaatt cttcttcgaa gcgtcccggg ttcagcgtgg cgcagttcat caccacgaac
     1321 ggcccggtcg atcggcggga ccgcgcgtgg atcatcctgg cgacgacctc tttcccggaa
     1381 ccggctggtc cggaaatcat gacccgcgac ccggtcggcg caacccgttc cacctgggat
     1441 cgcagttgaa cgatcagcgc tgaatccccc gtcagggtgg cctccgggcc ggcgcgaagc
     1501 cgcagttccg cattctcccg cgccagtgcg gcggcttcca gcgcccgcct tacgaccagc
     1561 agcaggcgat cggactggaa cggcttttcg ataaaatcgt atgcacctag ctggatcgcg
     1621 ccgaccgcca cttcgatggt gccgtggccc gagatcatca ccaccggcac atgcggttcc
     1681 tcgctgtgca gcgtttccag gatagcgatg ccgtccatgc gcgacccctg cagccacacg
     1741 tccaggatca ccagcgatgg ccgccgcacg cgaaacgcgg cgatcgccga ttcggcatcg
     1801 ccggcaccgc gcgtttcgta gccttcgtcc cgcaggatgc cttcgatcag cagccggatg
     1861 tcgggttcgt catcgacgat caggatttca tgcgccatgc gacacctgcg tcatccggcc
     1921 gttcggcccg ttctcgccgt gggcttgccc gttggacgtc gcggcaacgg ccatggcccg
     1981 aggcaacacc aggctggcca gggcgccggg gccattcggc tggtcctcga gcatcaaggc
     2041 cccgccatgg tcttccatga tctttttcac gatcgccaat cctaacccgg ttcccttcgg
     2101 cttgtgcgtt acatacggtt cggtcaaccg cgcccgatcc tcttgcggca gcccgactcc
     2161 atcatcaccg acgatgagac gtgctgcgtc cggcaggtct tgcaccgtca atgtgatgtg
     2221 accagcgcct tcgcgcgccg ccacggcatc ggcggcgttc tgcaacaaat tcgtcagcgc
     2281 ttgccgcagc atacgccggt cgcatggcac caccggtcct cgttccgcga tatccgtcac
     2341 ccattcaatt cctggcctgg cggtcttttg caacaccagc gcttcgcgtg cgatgcggcc
     2401 gacatcctcc ggcttgatca ccggctgcgg catccgggcg aaggccgaaa actcatcgac
     2461 catgcggccg atgtccccga catggcggat gatggtgtcg gcgcactgcg cgaatgtatc
     2521 cgggtcggac tggatttcct tggtgaagcg ccgcttcaac cgttccgccg atagctggat
     2581 cggggtcagc gggttcttga tctcgtgcgc gatccgccgg gccacatccg cccacgccgc
     2641 cttgcgctgc gccgattgca gttcggtgat gtcgtcgaac gtgacgacga agccgtccgt
     2701 ccgtccacca gaaagttcgg cgccgatccg aaccagcagt atccgccggt gattcaacgg
     2761 gccggtctgg atctctgcgg tccttgcttt gtcgggcgat gccgcgacct cgttgaacaa
     2821 cgccgcgaac tccggcacga tttccgccag cggatggcca gtcgcggcca gcaggtccaa
     2881 ccccagcaac tcatcggcgg cccggttcgg caactcgatc cggccctgcg catccaggcc
     2941 gatgacccct gccgatacgc cggacagcac agtctcggtg aagcgccgcc gctcatcgat
     3001 ctggctgtag gcgtccatca attcggcgcg ctgggcggcc aactggccgg tcatgcggtt
     3061 gaacgcccgg gacaggccgg ccagttcatc gccggtcgcc ggttccttga cccgcaccgc
     3121 gaggtcgccg ctgcgcaccc gttcggccgc gctgatcagc gtgcccacgg gccgagcgat
     3181 ctggttggcg atgatcaggc cgatcaaccc cgcggccaac aggaccagca gcgccacgat
     3241 cgcgaagatc caggcgaacg ccacctgcag ccaggaccgg ttgacatcca ggcgctggta
     3301 ttccgacacc gcctgttcgg tgcggcgcat gtagcccagg atcgtcgggt cgatcggccg
     3361 cccgatcatc agcatcagcg ccggcgtcga ttccagccgc acaaccgccc tgacacgggt
     3421 tccgtcgccg gcattcagga tcgccacgtc gccgttcatc gcctgctgcg ttgcggcggc
     3481 ggacggcgct tccaccccga ggccgacgaa aataccggcg gcggccagca cctgcccggt
     3541 cgtcggttcg tagatgatgg cctcggtcag gccgcgcagc gtcgtctggg tatcgagaac
     3601 ctcggcgaac accgtcggat cggcggacag gaaccgaccc gcgcgcgcca aatcgttcga
     3661 catctccagt gccaccgccc ggatattgtt gcggtgctcc tccaggtagc cgcgcgaaac
     3721 ctgtagcgac tccgccagcg actcacgcac cggctcgttg aaccaggatt ggatgccgaa
     3781 gtgaaagaac gcgaccgcga agcaggcaac gacaatggtc ggcgcgacgg ccacgccgct
     3841 gaacaggaac accagtcgga catgcaagcg cgatcccgcc gatccgcgcc gccgttccac
     3901 ccagacacgg gtcagccgcc ctgccagcac ggcgcccagc agcagcagca ccgacaggtt
     3961 cgccaacacc aggccgacgc cgacgcccgg cctccagccc aaaggcgatc cacgtgcgag
     4021 gatgatgaac gtcgccagtc caaccatcag cgccagcccg gtcagcacca gcgtcacggt
     4081 cttgcccagc atgatgtcgc ccagttggcc agcacgcgtg gatcgtggtt ccgaaatgat
     4141 atcccgcatg cggctggacc tgaactcggt cctcggggtt gagacgttca tgtcgtctgt
     4201 catcggcacg cagatccgat catctacatc aggccgcgca cgaccgggat ttccagatcc
     4261 cgtatcttct tgcgcagcgt gttgcggttg aggcccagca tcgatgccgc cttgatctgg
     4321 ttgccgcgcg tcgccgccag cgtgaggcgg atcagcggcc gttcgacctc ggcgatgatc
     4381 ttgtcgtaaa cgtcggtcac gcccatgccg tcgttgtgcg cggccaggaa ctccttgatg
     4441 tggcgctcga ctgcgctcga cagggtttca ggaccgttac tttcctgaac agaacctatg
     4501 ggcccctggg ccggcagcgg atcggtgagt tccgcgtcga tgaccgccgc gccgacgacc
     4561 tcgtccgggc acagcgcggc cagccgccgc atcaggtttt caagctcgcg gacgtttccc
     4621 ggccaggcgt gctgcttcag ccggtcgacc gccccttgat caagactttt ggccggaagc
     4681 ccatcctccc gcgcacgctc aaggaagtgg cgggccagca ccgctatgtc ctccacgcgc
     4741 tcgcgcagcg gcggcacccg gatcggcacg acgttcagcc ggtagaacag gtcctcgcga
     4801 aattgtccct gccggatggc ggtgcgcagg tcgcgatgcg tggcagcgat gatccggacg
     4861 ttggccttga tcggctgccg tccgccgacg ctagtgaact cgccctcctg cagcacccgc
     4921 agcagcctgg tctgcgcttc cggcggcatg tcgccgatct cgtccaggaa cagggtacca
     4981 ccgttggcct gctcgaatcg cccctgcgaa cggttggtgg caccggtaaa ggcgccgcgc
     5041 tcgtggccga acaactcgct ttcgatcagt tcgcgcggga tcgcggccat attgatggca
     5101 acgaacgaac cggaccgccg tttcccgtaa tcatgcagcg ccctggccac gagttcctta
     5161 ccggtgccgg actcgccgtt gatcatcacc gtcaggtccg ccgtcgtcag ccgggcgatg
     5221 gtgcgataga tctcctgcat cgccggactg cggccgatca gcggcagccg ctcctcggta
     5281 tccttcgcct ccaccgggct gaacgtcggt tcggacggtg ccgccagcgc ccgcccgacc
     5341 accgacagca actcgcgcag gtcgaacggc ttcggcagat actcgaacgc cccgcgctgt
     5401 gtcgccttca ccgccgtcat cagcgtcgac tgcgcgctca tcaccaccac ccgcagttcc
     5461 ggccgaatct tcttgatccg cgggat
//
LOCUS       DASWQN010001043         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_496259, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001043 DASWQN010000000
VERSION     DASWQN010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496259"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..>1563)
                     /locus_tag="VGC82_17820"
     CDS             complement(268..>1563)
                     /locus_tag="VGC82_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY0425181.1"
                     /translation="QTRKDFDMSNRLPLGMAEATRLTQAGDLMGATALIQRLLQGGDV
                     PEPAHTGSGSPTIIDVEPVVLDDTDTQPTARLSATAAQPASEFAGKFYAKPRAGLAET
                     LRDLAAKHMPAGLDIGGLSGGLGGGFGGLTGIPGLRSVSEPLPDGATFETATFTNPAG
                     TRDYKLYVPASRTGQPMPLIVMLHGCTQSPDDFAAGTGMNALAEEHGCLVAYPAQPSS
                     ANANKCWNWFNANDQGRDRGEPSLIAGITRQIMKDHPVDPSRVYVAGLSAGGAAAAIM
                     GAAYPELYAAVGVHSGLPVGAARDIPSAFVAMSQGATGSGLSTRVIPTIVFHGDKDFT
                     VHASNGDAVVAQSKATATGLRSTVQRGQAPGGHAYSRTIHTNPSGKALCEQWTIHGAG
                     HAWVGGSSSGSYTDPRGPNASQEMMRFFFEHRNAVTMTV"
BASE COUNT          257 a          485 c          549 g          272 t
ORIGIN      
        1 acggaggggc gaatttccct gtgatctcat cggccgcccg taccgcgcga gcttattggg
       61 cgcattagag ccgtttccgc ccccctcttg gccccttccg gacagtgaat cagtacgata
      121 ggtccgggga tcgccaaaga aaaaggccgc ggtttcccgc ggccgaagat agatcaaccc
      181 ttttctctga agcctgcgcg gctgaaagtc cgatgctgag ccggatcagt tccgaccagg
      241 accgccagga ggcttccaac atgatcccta cacggtcatg gtcacggcat tccggtgctc
      301 gaagaagaag cgcatcatct cctgcgaggc gttcggcccg cgcggatcgg tgtaggagcc
      361 ggatgaactg ccgcccaccc aggcgtgccc ggccccgtgg atcgtccatt gctcgcacag
      421 cgccttgccg gagggattgg tgtggatggt gcggctgtag gcatggccac cgggcgcctg
      481 accgcgctgg acggttgacc gcagcccggt cgcggtcgcc ttggactgcg cgacgaccgc
      541 atcgccgttg ctcgcatgga cggtgaagtc cttgtcaccg tgaaacacga tggtcgggat
      601 gacacgggtg ctcagaccgc ttcccgtcgc cccctggctc atggcgacaa aggcggaggg
      661 aatgtcgcgg gcggccccga ccggcaggcc ggagtgcacc ccgaccgcgg cgtaaagctc
      721 gggataggcc gcacccatga tggccgccgc cgcaccgccg gcggaaagcc cggcgacata
      781 gacgcgactt gggtccaccg gatggtcttt catgatctgc cgggtgatgc cggcgatcag
      841 cgagggctca ccccggtccc gaccctggtc gttggcgttg aaccagttcc agcatttatt
      901 ggcattggcc gaagagggct gggccgggta tgccaccaag caaccatgct cctcggcaag
      961 cgcattcatg ccggtgccgg cggcgaagtc gtcgggcgac tgggtacagc cgtgcagcat
     1021 cacgatcagc ggcattggct ggccggtgcg gctggccggg acgtagagct tgtagtcgcg
     1081 cgttccggcc gggttggtga aagtcgccgt ttcgaaagtg gcgccgtcgg gcagaggctc
     1141 ggaaacggag cggagaccgg gaatgccggt gaggccgccg aacccaccac ccagtccacc
     1201 gctgagtccg ccgatgtcga gaccggccgg catgtgcttg gcggcgaggt cacgcagggt
     1261 ttcggcaagg ccggcccggg gcttggcgta gaacttgccg gcgaattcgc tcgccggctg
     1321 agcggcagtg gcgctcagcc gggctgtggg ctgcgtgtcg gtgtcatcga gaacgaccgg
     1381 ttccacgtcg atgatggtgg gggagccaga gccggtgtgc gccggctctg gaacatcgcc
     1441 gccctggagc aggcgttgga tgagggccgt cgcccccatc agatcgccgg cttgggtgag
     1501 gcgggtcgct tcggccatcc cgaggggaag tctgttggac atgtcgaaat ctttccgtgt
     1561 ttg
//
LOCUS       DASWQN010001044         4289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_101198, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001044 DASWQN010000000
VERSION     DASWQN010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101198"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1206
                     /locus_tag="VGC82_17825"
     CDS             151..1206
                     /locus_tag="VGC82_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425182.1"
                     /translation="MIGKFDLITARAEPKRLQVRNHWLVSTFILALILLLPIIAPAAE
                     PGPLVLDSTIPLPDVRGRIDHMAIDLRRGRLVVAALGNDTIEVVDLAAGKPRQRIGGL
                     KEPQGVAYADKADAIFVASAGDGAVRIFGADDLKPLGRIDLKNDADNIRIDPRNANIV
                     VGYGDGGLAIIDPLTRTVIGTVLLQGHPEGFQIEPMTGRAYVNVPDAKQIAVVDLDGR
                     RQIATWKVPGAGSNFPMALDPAESLLAAVFRNPPRLVLLDTKTGAVAANLPTCGDADD
                     VFFDAKRARIYISCGAGEIAVFQRDGPTYRPLPSVPTASGARTSLFVPELDRLFLAVR
                     AGLLGSAASIQVFRPTP"
     gene            1225..2277
                     /locus_tag="VGC82_17830"
     CDS             1225..2277
                     /locus_tag="VGC82_17830"
                     /inference="COORDINATES: protein motif:HMM:NF033816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cj0069 family protein"
                     /protein_id="HEY0425183.1"
                     /translation="MTAGKIALLWRGDRQARQEATADNNRFSRVFAALTAHGIHAEPA
                     VYDEDNVDKVRDQLLKLDGVLVWVDPISKAGRDRSILDTMLRDVAGRGTWVSAHPDVI
                     LKMGVKEVLYRTKQLGWGTDTHLYRTADAFDEAFPRRLQAAGPRVLKQNRGNGGQGVW
                     RVELVSSTAIRVPTVRVLEARRGSVPEDVALADFMQRCQTYFGAAGCIIDQPFQPRLP
                     DGMIRCYMGGDKVIGFGHQLIKALILPPPEGSDSQAAQPGPRIMHGASAPEFQSLRTK
                     MELEWTPQMMRLLDIDAASLPIIWDADFLYGPRTAAGEDTYVLCEINVSSVAPFPDQA
                     PEEIARLAVARLRPPL"
     gene            complement(2312..2815)
                     /locus_tag="VGC82_17835"
     CDS             complement(2312..2815)
                     /locus_tag="VGC82_17835"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0425184.1"
                     /translation="MTGPTEPLSFTFDDDGAIPNSRLPLLVYRDAVPADAAAIEGVFT
                     ANRWPPAWRDGVHPFHHFHSSAHEVLGVARGEAAVLFGGPGGKVLTVRAGDVVVVPAG
                     VAHCNQGQSGDLLIVGGYPENAPDPDLRRGKPAEHDAAKQAVDRVPLPPADPVQGAGG
                     ALSRLWG"
     gene            complement(2817..3857)
                     /locus_tag="VGC82_17840"
     CDS             complement(2817..3857)
                     /locus_tag="VGC82_17840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEY0425185.1"
                     /translation="MREMPHPATWLKVLPNGLFCEPGGFFIDPLRPVDRAVITHGHSD
                     HARPGHRAVLATAETLTVMRARMGDENAGLSQQALGWHEPVTLNEVRVWLSPAGHVLG
                     SAQVAMEYRGSRAVVSGDYKRVADPTCVGFEPVPCDVFVTEATFALPVFVHPPPGDEI
                     KRLLDSVALFPDRTHVVGCYALGKCQRVIALLRQAGWDRPIWLHGALASMCEVYRQLG
                     VELGDLRPATVAAKAELVGAIVLAPPGAIADRWARRLEDPVVALASGWMRVRQRAKSR
                     GVELPLVISDHADWNELNATLDEVGAPEVWVTHGREEALIHAAGQRGIRGRALRLLGY
                     GDEDEAEATEAA"
BASE COUNT          765 a         1438 c         1344 g          742 t
ORIGIN      
        1 ggagggcgac cggctttccg ccgccttcaa tggaaagcaa ctgctcagcg tgtcagatcg
       61 gacgttcatc gggctcggga aggtagcgct ctggaccaag gcggacagtg tcacgcactt
      121 ctccgatctg acgataacgc cgttgccctg atgatcggaa aatttgacct gatcacggct
      181 cgcgcggaac ccaagaggct gcaagtacgc aaccactggc tggtctccac cttcattctg
      241 gcgctgatat tgctactgcc gataatcgct ccagcggcgg agcctggtcc attggttctc
      301 gactccacga tcccactgcc tgatgtgcgg ggacgtatcg atcacatggc aatcgacctc
      361 cgccgcgggc gccttgtggt ggcggcgctt ggaaatgaca ccatcgaagt ggtcgatctc
      421 gccgccggca agccgcgtca gcgcattggc ggcctgaagg agccgcaggg cgtcgcctat
      481 gccgacaaag ccgatgccat tttcgtggcc agcgccggcg atggtgccgt caggattttc
      541 ggtgccgacg accttaagcc gctcggccgg atcgacctga agaacgacgc cgacaacatc
      601 cgtatcgatc cgcgcaacgc caacatcgtc gtcggttatg gcgatggcgg cctggcaatc
      661 atcgaccctt tgacgcgcac cgtgatcggc acagtcctcc ttcagggaca ccccgagggg
      721 ttccagatcg agccaatgac cgggcgcgcc tacgtcaacg tcccggatgc aaagcagatc
      781 gcggtcgtcg atctcgatgg gcggcggcag atcgcaacct ggaaggtccc cggggcgggc
      841 agcaactttc ccatggcgct cgatcccgcg gaatctttgc tggccgcggt gttccgcaac
      901 ccgccccggc tggtgctgct tgacacgaag accggcgctg tcgcggccaa tctgccaacc
      961 tgcggcgatg cggatgacgt gtttttcgat gccaagcgcg cgcgtatcta catcagttgc
     1021 ggcgccggtg agatcgcggt gtttcagcgg gatggtccga cctatcggcc gctgccctcc
     1081 gtccccacgg cgtcaggcgc gcggacaagc ctgttcgtgc cggagctgga tcgcctgttc
     1141 ctcgcggttc gcgccggact gctcggttcc gctgcgtcga tccaggtctt tcgcccaacg
     1201 ccgtagaact ccggagattg taccatgacc gcaggcaaga tagcgttgct ttggcgcggc
     1261 gatcgccagg ccaggcaaga agcgaccgcc gataacaacc ggttcagtcg cgtgttcgcg
     1321 gcgttgaccg cgcacggtat ccatgccgaa cccgcggtct atgacgagga taacgtggac
     1381 aaggtgcgtg accagcttct gaagctcgat ggcgtgctcg tttgggtcga tccgatatcg
     1441 aaagcgggcc gggacaggtc cattctcgac acgatgctgc gcgacgtcgc cggtcgcgga
     1501 acctgggtca gtgcccatcc ggacgtcatc ctcaagatgg gtgtgaagga agtcctttat
     1561 cgcacgaagc aactgggctg ggggaccgat acgcatcttt accggaccgc cgacgccttt
     1621 gacgaggcgt ttccgaggcg gcttcaagcg gccggtccgc gcgttctcaa gcagaaccga
     1681 ggcaatggcg gccaaggcgt atggagggtc gaactcgttt cttccacggc catccgcgtg
     1741 ccaacggttc gcgtgctcga ggcgcgtcgg ggcagcgtgc cggaagacgt ggcgcttgcg
     1801 gatttcatgc agcgttgtca gacatatttt ggcgctgcgg gctgcatcat cgatcaacca
     1861 tttcagccgc ggctgccgga cggcatgatc cgctgctaca tgggtgggga caaggtgatc
     1921 ggtttcggac atcagctcat caaggcactg atcctgccgc cgcccgaggg ctcggattcg
     1981 caagcggccc aaccaggccc gcggatcatg cacggtgcct cggcgccgga attccagtcc
     2041 ctgaggacaa agatggaact ggagtggacg ccgcaaatga tgcgtctttt agacatcgac
     2101 gcggcgtcgc tgccgatcat ctgggatgct gattttcttt atgggccacg caccgcggcc
     2161 ggcgaggaca cttacgtgct ttgcgagatc aatgtcagtt cggttgcgcc gttcccggac
     2221 caggcacctg aggaaatcgc gcgactcgct gtggcacgcc tacgaccacc gctatgaatg
     2281 cggccatgga tcggacatcc tgaaggcggc atcagcccca aagccgggac agcgctccac
     2341 ccgcgccctg gaccggatcg gccggcggca acggcacccg atcgaccgcc tgttttgccg
     2401 catcatgttc cgccggcttt ccgcgccgca aatccgggtc gggcgcattt tccggatacc
     2461 cgcccacgat cagcaagtca ccggattgac cctggttgca gtgcgcgacc ccagccggca
     2521 ccaccacgac atcgccggcc cgcacggtca gcactttccc gcccggcccc ccgaacagca
     2581 ccgcggcctc gccccgggca acacccagga cctcgtgcgc agaactgtga aaatgatgga
     2641 acgggtgtac cccgtcgcgc catgccggcg gccagcggtt ggcggtaaac acaccttcga
     2701 tcgccgctgc gtccgccggc accgcatcgc gatacaccag cagcggtaaa cgggaattcg
     2761 ggattgcccc gtcgtcgtcg aacgtgaaac tcagcggttc tgtcggtccg gtcatgtcag
     2821 gctgcctccg ttgcttctgc ttcatcctcg tcgccatagc cgagcaaccg caacgcccgg
     2881 ccgcggatgc cgcgctggcc ggctgcgtgg atcagcgctt cctcgcgtcc atgcgtcacc
     2941 cacacctccg gcgcgccaac ctcgtcgagg gtcgcgttca gctcgttcca gtcggcatgg
     3001 tcggagatca ccagcggtaa ttcgaccccg cgcgacttcg cccgctgccg tacgcgcatc
     3061 cagccggaag ccagagcgac caccgggtcc tccagccgcc gcgcccagcg gtcggcgata
     3121 gcgccgggcg gagccagcac gatcgccccg accagttcgg cctttgccgc caccgtcgcc
     3181 ggccgcagat cgcccagctc gacaccgagt tgccggtaaa cctcgcacat cgaagccaag
     3241 gcgccgtgca accagatcgg ccggtcccat cccgcctgcc gcagcagcgc gatcacgcgc
     3301 tgacattttc ctaatgcgta gcagcccacg acatgggtcc ggtccggaaa cagcgccacg
     3361 ctgtccagca gccgcttgat ttcatcgcct ggcggcggat gcacgaatac cggcagcgcg
     3421 aacgtcgcct cggtgacgaa tacgtcgcac ggcaccggct cgaacccgac gcatgtcgga
     3481 tcggccactc gcttgtagtc gccgctgacc accgcccgcg acccccggta ttccatcgcc
     3541 acctgggcgc tgcccagcac atgtccggcg ggcgacagcc agacgcggac ctcgttcagc
     3601 gtgaccggtt cgtgccatcc cagcgcctgc tgacttaggc cggcgttttc gtcacccatc
     3661 cgagcgcgca tcaccgtcag ggtctcggcc gttgccagta ctgcgcggtg gcccggacgg
     3721 gcgtggtcgg agtggccatg ggtgatcacc gcccggtcca ccggccgcag cgggtcgatg
     3781 aagaacccgc ccggttcgca aaacaaaccg ttgggcagaa ccttgagcca ggtggcggga
     3841 tgcggcatct cccgcatgtt ggcagctgtc gcggttttga ccagcccgcg gcacgcttta
     3901 gctgttgcgg cctggtggcg cttcttctgc tgcgacgcag ttcaatggag cacgccaagc
     3961 tgatcaacca ggcccagcac attggcgccg ccaaccgcgg cgaccacgca gagcagcaca
     4021 ggcgccgccg acggcggcga gcaggcccag cacacaggcg ccgccgacgg cggcgaccat
     4081 gcagagcagc aaggtgacca cgaggacggc gcgagatgct ccacgagcgc gaacaggtgc
     4141 tccaccgcga gggccggatg cgctttgcgg gctggggtgc caaatgaggc agtttgacat
     4201 gctcgggaat gccgatgccc tcataattga agcgggcgag gtccagggta agtcagggtg
     4261 gggcctaaac gcgaagatat taaccttcc
//
LOCUS       DASWQN010001045         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2212796, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001045 DASWQN010000000
VERSION     DASWQN010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2212796"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VGC82_17845"
     CDS             complement(<1..195)
                     /locus_tag="VGC82_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425186.1"
                     /translation="MRTLRSPQRAEAVLPGVALAMALRSDPTDTTGGASVGGSILTGA
                     ISPFPSSELRQRRIELLMVEI"
     gene            188..970
                     /locus_tag="VGC82_17850"
     CDS             188..970
                     /locus_tag="VGC82_17850"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY0425187.1"
                     /translation="MRILSAAVLAPLALGCIWMGGTAFAALVALISAGLSFEWLGFCH
                     QSKKAVTIVLFAALPLSVMLTASGATVPAFGLLAVAMVAGIALAHGLSPSRPLAFGIP
                     YLGLGAVALVWLRQSQAAGGANVIVLLLIVWASDIGAYAAGRAIGGPRLAPAISPGKT
                     WSGAVGGLAAAAATGCAAAAFLDNGAPSWRPAVFALLIGAIGQGGDLFESQLKRHFGV
                     KDSGRMIPGHGGLLDRLDAVLTAAPAAALLALTLGRGVVLWE"
     gene            967..2199
                     /gene="dxr"
                     /locus_tag="VGC82_17855"
     CDS             967..2199
                     /gene="dxr"
                     /locus_tag="VGC82_17855"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY0425188.1"
                     /translation="MNTGHPSFAQRGSARAAPAQASAVRSVTVLGSTGSVGTQTIELL
                     AADSDSFRVRALVAGRNTTLLAQQAIALRAEVAVVSDPRAYAALRDALAGTGIEVAAG
                     AEAVVEAACLQADWTMAAITGAAGLASTLAAIRRGGYVALANKEALVCAGDVMLRAVR
                     EAGATLLPVDSEHNAIFQSLADGNRGAVEKIVLTASGGPFRTASLEDMARATPDAALR
                     HPVWSMGAKISIDSATMMNKGLELIEAARLFDLDETAIDVLVHPQSIIHGMVYYHDGS
                     VLAQLGSPDMRIPIAHTLAWPRRMATTSRRLDLAAIGRLEFAPPDLIRFPALRLAREV
                     LQAGGGTPTILNAANEIAVEAFLQRRVGFLDIAETVSRVLQQLGTPRADTLDDVIGLD
                     ATARRLAQTLMLTPVSVS"
     gene            2227..>2580
                     /locus_tag="VGC82_17860"
     CDS             2227..>2580
                     /locus_tag="VGC82_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0425189.1"
                     /translation="MNIFPDFIRSAAAFVVVLGVLVFVHELGHYLAARWRGVRVETFS
                     IGFGRAITSWTDSRGTVWKLAWLPLGGYVKMHGQERPEDVSEEVRKSWLPGQTFHDKT
                     VLSRAIVVAAGPIANF"
BASE COUNT          389 a          810 c          854 g          527 t
ORIGIN      
        1 gatttcgacc atgagcagtt caatgcggcg ctgacggagt tcgctcgacg ggaacggcga
       61 tatggcgccc gtaaggattg agccgccaac agaggccccg ccggtggtat cggtagggtc
      121 ggaccgaagc gccatcgcca acgcaacacc cggcagcacc gcctccgccc gctggggcga
      181 cctcagggtg cgcatcctgt ccgcggcggt tctggctccg ctggctcttg ggtgcatctg
      241 gatgggcggg acggcttttg ccgccctcgt tgccctcatt tccgccggcc tttcctttga
      301 gtggttgggt ttttgtcacc agagcaagaa ggccgtcacc attgtcctgt tcgctgccct
      361 gccgctgtcg gtgatgctga cagcttcagg tgcgacggtg ccggcctttg gtctgctggc
      421 ggtcgcaatg gtcgctggaa tcgccttggc gcacggactg tctccgtcgc ggccacttgc
      481 atttggcatt ccctatcttg gtcttggcgc tgtggcgttg gtgtggctcc ggcagtcgca
      541 ggccgcgggt ggcgccaatg tgatcgtgct gctgttgatc gtatgggcta gtgacatcgg
      601 cgcctatgcc gcgggacggg cgatcggcgg accccggctc gcgccagcga tatcgccggg
      661 gaaaacctgg tcgggagccg tgggcggctt ggccgccgca gccgccacgg gttgcgccgc
      721 ggccgccttc ctggacaacg gggcgccgtc gtggcgtccg gcggtcttcg cgttgctgat
      781 cggcgcaatc ggtcagggcg gcgatttgtt cgagagccag ttgaagcgcc acttcggcgt
      841 caaggattcc ggccgcatga tcccgggcca tggcggcctg ctggatcggt tggatgccgt
      901 gttgaccgcc gcgccagctg cggccttact ggcgttgacc cttggacgag gagtagtgct
      961 gtgggaatga acactggtca cccgtccttt gcccaacgcg gttcggctcg cgccgctccg
     1021 gcccaggcta gcgcggttcg ttccgtgacg gtcctcggca gcacaggcag cgtcggaaca
     1081 caaacaatcg aattactggc agcagattcg gacagcttcc gggttcgggc actggtcgca
     1141 ggccggaata cgacgctgct ggcccagcag gcgatcgcct tgcgcgccga ggtcgcggtc
     1201 gtttcggacc cccgggcgta tgctgccctg cgcgacgccc tcgcgggcac tgggatcgag
     1261 gtcgccgccg gtgccgaagc ggtcgtcgag gccgcctgcc tgcaggctga ctggacgatg
     1321 gcggcgatca ccggcgccgc tggcctggca tccaccctcg ccgcgatccg gcgcggcggc
     1381 tatgtcgccc tggccaacaa agaagccctg gtctgtgccg gcgacgtgat gctgcgtgcg
     1441 gttcgcgagg ccggcgccac gttgctgcct gtggattccg agcacaacgc catttttcag
     1501 tcacttgccg acggtaatcg cggcgctgtg gaaaagatcg tgctgaccgc atcgggtggt
     1561 ccgttccgta ccgccagcct cgaagacatg gcccgggcca cgccggacgc cgcgttgcgt
     1621 catccggtct ggtcgatggg tgcaaagatc agcatcgatt cagcgaccat gatgaacaaa
     1681 gggctggaac tgatcgaggc tgcgcggctg ttcgacctgg acgagaccgc gatcgatgtc
     1741 ctggtgcatc cgcaatcgat cattcatggc atggtctatt accacgatgg ctccgtgctg
     1801 gcgcagcttg gttcgcccga catgcgaatt ccgattgctc atacgctggc ctggccgcga
     1861 cgcatggcga cgacttcgcg ccggctcgat cttgcggcga tcggccgcct tgaatttgcc
     1921 ccacccgatc tgatacggtt ccctgcactt cgtttggcaa gagaagtatt gcaagctggg
     1981 ggcggaaccc ccaccattct gaacgcggcg aacgagatcg ccgtcgaggc tttcctgcaa
     2041 cgtcgcgttg gtttcctgga cattgcggag acagtcagcc gcgtcctgca acaactgggt
     2101 acgcccaggg cagacacgct ggacgacgtg atcggccttg acgctaccgc ccgtcgtctc
     2161 gctcaaaccc tgatgcttac gcctgtctct gtttcgtaat cacttttcgc cgagagccaa
     2221 accgtgctga acatttttcc cgacttcatt cgcagcgcgg cagcattcgt cgtcgtactc
     2281 ggcgtgctgg tcttcgtcca tgagcttggc cactaccttg ccgcccgttg gcgcggggtt
     2341 cgcgtggaaa cattttccat cggctttgga cgggcgatta cgtcctggac cgacagccgg
     2401 ggcaccgtct ggaagctggc ttggctgccg ttgggcggtt acgtgaagat gcacggtcag
     2461 gaacgtcccg aagacgtgtc cgaggaggtg cgcaagagtt ggcttcccgg ccagacgttc
     2521 cacgacaaga cagtattatc ccgggcgatc gtggtggcgg cgggcccgat cgccaatttt
//
LOCUS       DASWQN010001046         3381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2080962, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001046 DASWQN010000000
VERSION     DASWQN010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080962"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VGC82_17865"
     CDS             complement(<1..709)
                     /locus_tag="VGC82_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HEY0425190.1"
                     /translation="MNISFDNSYARLPDRFYARLNPTPVAEPKLVRVNAALAETLGID
                     PAELASPEGVAVLAGNRVPAGAEPMALAYAGHQFGNFVPQLGDGRANLLGEVVGRDGK
                     RRDIQLKGSGPTPFSRRGDGRAALGPVLREYLVSEAMAALGVPTTRTLAAVTTGETVV
                     RETMLPGAVLTRVAASHIRVGTFQYFAAQGDQEAVRHLADYAIARHYPEAASAPDPYR
                     AFLEGVVARQAGLVARWL"
     gene            789..1796
                     /locus_tag="VGC82_17870"
     CDS             789..1796
                     /locus_tag="VGC82_17870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0425191.1"
                     /translation="MIPLSILDLSPVPEGSNAGQALRNSIDLARHVEALGYGRYWMAE
                     HHNMAGIASAATAVALAHVAVATSTIRIGAGGIMLPNHAPLLIAEQFGTLAALHPGRI
                     DLGLGRAPGSDQIAARAMRRNLAADVDQFPSDVVELMSYFEPTEPGQVLQAVPGAGEE
                     VAVWILGSSTFGAQLAAHLGLPYAFASHFAPGQMQDAVAIYRERFRPSARLAKPHVML
                     GVNIFAADTDAEARRLFSSLQQAFLNLRRGRPGKLPPPVDDLEARLDRYGRSMLADAL
                     ACSIVGGPETVRNGLQAFATSTGADELMVTAQIFDHQARKRSFEILAHEAGTHEVVKA
                     A"
     gene            1860..3008
                     /locus_tag="VGC82_17875"
     CDS             1860..3008
                     /locus_tag="VGC82_17875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY0425192.1"
                     /translation="MALGSLNEFSLTPDRRSVLKWTSALAGATGAGLATGQAQPVRHA
                     ETSPRPHQAGSLRKGMFGYTLAHEQFPVPELVRFGGMASRAGFPVLATSDHLQPWQAN
                     EGHSGQAWVTMGALGGQTHSWMGTAVTCPTMRYNPAVVAEGFATLSHLYPGRIFLGVG
                     SGEALNEQVATGMWPKWQERWDRLIEAITVIRQLWTGKDVSFKGQYYNVNAKLYDPPA
                     QPIPLFTAANGKKSMRLAGQHGDGLISDPKTWMQHKSEWEAGAREAGKNPADMPVMIE
                     HYVVVGDQQAAKQAAELWRFGPKAFKGLYNVPSPVEIQQKAEAASPIDEVTQGWAIGT
                     DPAVHIKKAHELFDSGVSIVNVHSGQPDQARVIDFYGTHVLPAFKNRA"
BASE COUNT          562 a         1080 c         1155 g          584 t
ORIGIN      
        1 gcaaccaccg cgccaccaga ccggcctgcc gggcaacgac tccttcaagg aaagcgcgat
       61 aggggtctgg cgcggaagcc gcctcggggt agtgccgggc gatggcataa tccgcgaggt
      121 gccgcaccgc ctcctggtcg ccctgcgcgg cgaaatactg gaacgtgccg acgcggatat
      181 ggctcgcggc gactcgggtt agcacggcgc cgggaagcat cgtctcgcgc accaccgtct
      241 cgccggtcgt cacggcggcg agcgtccgcg tcgtcggcac accaagtgcc gccatcgcct
      301 cgctgaccag gtattcgcgc agaacagggc ccaacgcggc ccgcccgtcg cctcggcgcg
      361 aaaatggcgt cgggccggag cctttcagtt gtatgtcgcg gcgtttgccg tcgcggccga
      421 ccacttcgcc cagcagattg gcgcggccat cgccgagctg cggcacgaaa ttgccgaact
      481 ggtggccggc ataggccagc gccatcggtt cggcgcccgc cggcacgcga ttgccggcca
      541 gaacggcgac gccttcggga ctggcgagtt cggcgggatc gatgcccagt gtctcagcga
      601 gggcggcgtt gacccgtacc agcttcggtt cagccaccgg agtcgggttc agacgcgcgt
      661 agaagcggtc gggcaggcgg gcgtagctgt tgtcgaacga gatgttcatg tcgcgatcct
      721 ggtgtcctgc cgcggaaatg cgccggcgcg atattccgct ggcgcctgtt ccatcctatg
      781 tagggtggat gatccccttg tccattctcg atctctcgcc ggttcccgaa ggcagcaacg
      841 ccggtcaggc gcttcgcaac tccatcgacc ttgctcggca tgtggaggcg ctgggctacg
      901 ggcgctactg gatggcggag catcacaaca tggccggcat cgccagcgcg gcgacggcgg
      961 tggccttggc ccatgtggcg gtggcgacca gcaccatccg catcggcgcc ggcggcatca
     1021 tgctgccgaa tcacgcgccg ctgctgatcg ccgaacagtt cggcacgctg gcggcgctgc
     1081 acccggggcg gatcgacctc gggcttggcc gggcgcccgg atccgaccag atcgccgctc
     1141 gggcgatgcg gcgcaatctc gcggcggatg tggaccagtt cccgtcggac gtcgtggaac
     1201 tgatgagcta tttcgaaccc accgaacccg gccaggtgtt gcaggcggtt cccggcgccg
     1261 gtgaggaagt cgcggtgtgg atcctcgggt ccagcacgtt cggcgcgcaa ctggcggctc
     1321 atctgggcct gccctacgct ttcgcctcgc atttcgcacc gggacagatg caagacgcgg
     1381 tggcgatcta ccgcgagcgg tttcgcccgt cggcgcgcct ggcgaaaccc cacgtcatgc
     1441 ttggggtcaa catcttcgcg gccgatacgg atgccgaggc gcggcggctg ttttcgtcgc
     1501 tgcagcaggc gttcctgaac ctgcggcgcg ggcggccggg gaaactgccg ccgccggtgg
     1561 atgacctgga ggcgaggctc gaccgctacg gccggtcgat gctggccgac gcattggcgt
     1621 gctcgattgt cggcgggcca gaaaccgtgc ggaacgggct gcaagccttc gccacctcca
     1681 ccggtgcgga tgagctgatg gtcacggcac agatcttcga ccaccaggcg cggaaacggt
     1741 cgttcgagat cctggcgcac gaggccggaa cacatgaagt cgtgaaggct gcctgatcca
     1801 ggaccggaac tccggttcgc gggctccgtt ggtgccggat gcatcagtcg gagcatgaca
     1861 tggcgttggg tagcctgaac gagttctctt tgacgccgga ccggcgcagc gtgttgaagt
     1921 ggacgagcgc actcgccggc gcgacggggg ccggacttgc cactggccag gcgcaaccgg
     1981 ttcggcatgc cgagacatcc ccacgtccgc atcaggctgg cagcctgcgg aaaggcatgt
     2041 tcggctacac gcttgcccac gagcaattcc cggtgccgga gcttgtccgg ttcggcggca
     2101 tggcctcccg tgccggattt ccggtgctgg cgaccagcga ccacttgcaa ccgtggcagg
     2161 ccaacgaggg ccattccggc caggcctggg tcacgatggg cgcgcttggc ggccagacgc
     2221 atagctggat gggaaccgcg gtcacctgtc cgacgatgcg ctacaatccc gccgtcgtcg
     2281 cggagggttt cgcgacgctc agtcacctgt atccggggcg cattttcctg ggtgtcggct
     2341 ccggcgaggc cttgaacgag caggtcgcga ccgggatgtg gccgaaatgg caggagcggt
     2401 gggaccggct gatcgaggct atcaccgtca tccgccagct ttggaccgga aaagacgtgt
     2461 ctttcaaggg ccagtactac aacgtcaacg ccaagctgta cgatccgccc gcccagccta
     2521 ttcccctgtt caccgccgcg aacggcaaga agtccatgcg cctggcgggt caacatggcg
     2581 acggcctgat cagcgatccg aagacctgga tgcagcataa atccgaatgg gaagccggcg
     2641 cgcgggaggc gggcaagaat cccgccgaca tgcctgtgat gatcgagcac tatgtcgtgg
     2701 tcggcgatca gcaggcggcc aagcaagcgg ccgaactctg gcggttcggg ccgaaggctt
     2761 tcaagggatt gtacaacgtc cccagcccgg tcgaaatcca gcagaaggcc gaggccgcat
     2821 cgccgatcga cgaggtcacc caaggctggg cgatcggcac cgaccctgcc gtccatatca
     2881 agaaggcgca cgagttgttc gacagtggcg tcagcatcgt caacgttcac tccggtcaac
     2941 ccgaccaggc cagggtcatc gatttctacg gcacgcacgt gttgcccgcg ttcaaaaacc
     3001 gagcctgatg gcgccgctgg ggacaagcga gcggtgcgac gcgcttgctg tcctcggacg
     3061 tctgagacag tgatccgctg ccttacttcg gcgtcatgat ttggacatgg attgattgcc
     3121 gacgcgggaa taaatgcggg tgggagcggg ttttcgctgc cgtgcagcac ttgcgggaat
     3181 cgctgcgctg cccaattagg ctgggatgac agataaccgc tccgaaccgg gtgaagttca
     3241 gcaacgtcat ggcgtgcttc gcccgacgcg tgttggagca atcgctttcg ggcggccgtt
     3301 gcagcctgtg tcctggtggg cgccgtgttc gcttgtgtca ttgtcggctc ggtgccggcg
     3361 atccgcggct ttgccacgtt c
//
LOCUS       DASWQN010001047         3623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1684451, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001047 DASWQN010000000
VERSION     DASWQN010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684451"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VGC82_17880"
     CDS             complement(<1..1276)
                     /locus_tag="VGC82_17880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0425193.1"
                     /translation="MTDRAGGSIAADTLLILMPGARDAESAAALLAIEGVECRLCPDV
                     AALCAGIGGNAGAVLVADEALTTAVLEALSDRLETQPVWSDLPFIVITNENSAARRVA
                     TEPRLPDALGNVLLLEHSSRTLGLVSAVRSALRARRQQRQMGAYLTEQRNVAAALRES
                     EARFRHMADSVPALIWMTDATGDIVFVNMHYHHVFGRSVADIIRDGWRAIVVPDARRR
                     FRSSFAAAFQSRTAFTAETQVRDKAGRVRWLRCDGVPRLDDIGRFLGYTGCCLDITDT
                     RLATQELEWRVAGRTEELTSALKRLNAEVKEREQVEKALRQSQKMEAIGQLTGGIAHD
                     FNNMLQGIVGALDVIRKLESTGRTAGIPRFVDMAMNSAQRAAAMTHRLLAFARQQPLA
                     PQRVHPNELVQSLRELVRRTCGESIVLSLQLEP"
     gene            complement(1625..3028)
                     /locus_tag="VGC82_17885"
     CDS             complement(1625..3028)
                     /locus_tag="VGC82_17885"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY0425194.1"
                     /translation="MPAPESSVLASVLDHADANLAASRANLFDLLRIKSISAQPAHAA
                     DCIKAAEWWRDQLAGLGFDASVRPTAGHPVVVAHLSGPAGYTGPHILFYGHYDVQPVD
                     PLSLWHSDPFDPQLVDGPRGKRYVARGAVDDKGQTLMFLEALRAWNIAGGGIPARITV
                     LIEGEEEVGSVNLDPFLRANKAELAADMALISDTGMWNVDTPAITTRLRGMTYAEVTL
                     RAANRDLHSGLYGGSALNPINALTKILGGLQDENGRIQLPGFYDSVAAVSNSQRAQWD
                     ALGFDETAFLGGIGLTTPAGERGYSALERLWARPTADINGIWGGYTGAGSKTVIAAEA
                     SAKVSFRLVANQDPDAVMEQFKQFVTDRLPPGATATFADFARAPGIEVNVDSPHVRQA
                     LDALADEFGKPALLMGSGGSIPVVTSLRSILGIDSLLMGFGLDDDQVHSPNEKFEETC
                     FHHGIRSHVRLLAKFAA"
     gene            complement(3143..>3623)
                     /locus_tag="VGC82_17890"
     CDS             complement(3143..>3623)
                     /locus_tag="VGC82_17890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0425195.1"
                     /translation="TFVSADPNGVPAIGGTVLVVEDEADARAMVADVLRDLGCEVIEA
                     ADGNAGLRIVQSNGPLDLLITDVGLPGLNGRQLADAARETRPTLPVLLVTGYAGRALD
                     DAELEPGMEVLRKPFTFNVLVARVRAILMPASVVEGPNLGTLPVADAGLGRRRGVHD"
BASE COUNT          635 a         1213 c         1138 g          637 t
ORIGIN      
        1 cgggctccag ctgcagcgac agcacgatcg attctccgca ggtgcgccgc accagctcgc
       61 gcagcgactg caccagttcg ttgggatgca cgcgctgcgg cgccagcggc tgctgccggg
      121 cgaacgccag caggcggtgc gtcatcgccg ccgcccgctg cgccgaattc atcgccatgt
      181 cgacgaagcg cgggatgccc gcggtgcggc cggtggactc cagcttgcgg atcacgtcga
      241 gcgcaccgac gatgccctgc agcatgttgt tgaagtcgtg cgcgatcccg cccgtgagct
      301 ggccgatcgc ctccatcttc tgtgactggc gcaacgcctt ctcgacttgc tcgcgctcct
      361 tcacttcggc gttcaaccgc ttgagcgcgc tggtcagctc ttcggtgcgc cccgccacgc
      421 gccactccag ctcctgggtc gcaagcctcg tgtcggtgat gtccaggcag catccggtat
      481 agccgaggaa ccggccaata tcgtccaggc ggggaacgcc gtcgcaccgc agccaccgca
      541 cccggcccgc tttgtcgcgt acctgtgttt cagcggtgaa tgccgtccgg ctctgaaatg
      601 cagcggcgaa ggaggatcgg aagcgccttc gcgcatcggg tacgacgatg gccctccagc
      661 cgtcgcggat gatgtcggcg acggaacggc caaagacatg gtgataatgc atgttcacga
      721 agacgatgtc gccggtggcg tcggtcatcc agatcagcgc cggcacgctg tccgccatgt
      781 gccgaaaccg agcctcgctt tcccgcaacg ccgccgcgac gttgcgctgt tcggtcagat
      841 acgcgcccat ctgccgctgc tgccgtcttg cccttaacgc ggaacgcacc gcggaaacca
      901 ggccgagggt gcgggacgaa tgttccagca acagcacgtt gcccagcgcg tccggcaggc
      961 gcggttccgt cgccacccgc cgtgcggcac tgttctcatt cgttatgacg atgaacggaa
     1021 ggtcggacca gacgggttgg gtttcgagac gatcggacag cgcctcgagg acggcggtgg
     1081 tcaacgcttc gtcagccacc agcaccgcgc cggcattccc gccgatcccg gcgcacagcg
     1141 cggcgacatc cgggcagagg cggcattcga cgccctcgat ggcgagcagc gcggcggcgc
     1201 tttcggcatc gcgagccccg ggcatcagga tcagcaacgt gtccgccgcg atggaaccac
     1261 ccgcgcggtc cgtcatgcag atagccggtg cggttgccga tcacggcaac gccgcgcctg
     1321 accgccgttt tgacgaatac cgccgccgtt tttaccgccg ccgttttgac gaatcatgac
     1381 gttgaggacc gatgtggcaa caccgggtgc aagcttattt cgttatagca tgagctgagg
     1441 ccgtgacaca taattgctgt ttatctattt cgtcgaaaat tgcaacaacg tattgtcgcg
     1501 cgcaggcctt gctgggaacg ccaaggcccg gaagcacggc ttattcgttt ttttgcagtg
     1561 ccgatgcgtt acattgcctt attggcggcg gcgtcaacat gacggcatcg gagagaactt
     1621 gtttttacgc agcgaatttc gcgagaagcc ggacgtggga gcgtatgcca tgatggaagc
     1681 aggtctcttc gaacttctcg ttcgggctgt gcacctggtc atcgtccaac ccaaagccca
     1741 tcagcaatga atcgatgcca agaatcgacc gcagcgacgt caccaccggg atcgacccgc
     1801 cgctgcccat caaaagcgcc ggtttgccga attcgtccgc cagggcgtcc agtgcctggc
     1861 gcacatgcgg gctatcgaca ttgacttcga tgcccggcgc acgggcgaaa tcggcgaagg
     1921 tggcagtggc acccggcggc aggcgatcgg tgacgaactg cttgaactgc tccatgaccg
     1981 catcggggtc ctggttcgcc accagccgga acgatacctt ggcggaagcc tccgccgcga
     2041 tcaccgtctt gctgccggcc ccggtgtagc cgccccaaat gccattgatg tcagcggtcg
     2101 gccgagccca aagccgctcc agcgccgaat agccgcgttc gccggcgggc gtggtcaggc
     2161 cgatgccacc caggaacgcc gtctcatcga agcccagcgc atcccattgc gcccgctgcg
     2221 agtttgagac cgccgcgacg ctgtcataga accccggcaa ctggatgcgg ccgttctcgt
     2281 cctgcaaccc gccgaggatc ttcgtcagcg cattgatcgg gttcagcgcc gacccgccgt
     2341 acagccccga gtgcaggtcg cggttcgccg ccctcagcgt cacctcggca taggtcatgc
     2401 cgcgcaggcg cgtggtgatc gcgggcgtgt ccacgttcca catcccggtg tcgctgatca
     2461 gtgccatgtc cgcggccagt tcggctttgt tcgcccgtag gaacggatcg aggttgacgg
     2521 aaccgacctc ttcctcgcct tcgatcagca cggtgatacg ggcggggatc ccgccgccgg
     2581 cgatgttcca cgcgcgcagt gcttccagga acatcagcgt ttggcctttg tcgtccaccg
     2641 ccccccgggc gacgtagcgc ttgccgcggg ggccatcgac cagttgggga tcaaacggat
     2701 cgctgtgcca cagcgacagc ggatcgaccg gctgtacgtc gtagtggccg tagaacagga
     2761 tgtgcggccc ggtgtagccg gccggcccgc ttaggtgcgc gactacgacg gggtgaccgg
     2821 cggtcggacg aacggaagcg tcgaacccca gcccagccaa ttgatcgcgc caccactcag
     2881 ccgccttgat gcagtcggcg gcatgggccg gctgggcgct gatcgacttg atgcgcagca
     2941 ggtcgaacag gttggcacgc gatgccgcca gattggcgtc ggcgtggtcc aggaccgagg
     3001 caagaacgga cgattcgggg gccggcatgg gattctcctg atctgatgcc aacaatcggt
     3061 cgatgaacgc ctgctgggcg atcgtgatcc aggtaccgaa gctggtctgg gtcgaaatgt
     3121 ctggttcgat tcgacccgat cgtcaatcgt gcaccccgcg acgccggcct aacccagcgt
     3181 cagctaccgg cagggtcccc agattcggcc cctccaccac ggatgcgggc atcaggatcg
     3241 cgcgcacgcg ggcgacgagc acgttgaaag taaatggctt gcgcaacacc tccatacccg
     3301 gctcaagttc agcatcatcc agcgccctgc cggcataccc ggtgaccagc agcaccggca
     3361 acgttggccg ggtttcccgt gcggcgtcgg caagctggcg cccgttcagt cccggaaggc
     3421 ccacatcggt tatcagcaag tccagcggac cattcgactg gacgatccgc aatccggcat
     3481 tgccgtccgc ggcctcgatc acctcacagc cgaggtcgcg cagcacgtcg gccaccatgg
     3541 cacgtgcgtc ggcctcgtcc tcgacgacca gcaccgtccc gccgattgcc gggacaccgt
     3601 tcggatcggc tgaaacaaaa gtc
//
LOCUS       DASWQN010001048         5680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_496839, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001048 DASWQN010000000
VERSION     DASWQN010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496839"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VGC82_17895"
     CDS             complement(<1..926)
                     /locus_tag="VGC82_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY0425196.1"
                     /translation="MFGDAEPAAPPEPAINRPLADRLRPATLSEVVGQDHLLGPEGSL
                     TRMLERGSLASLILWGPPGVGKTTIARLLASRAKLAFVQLSAVFSGVADLKRAFDDAT
                     KRRRAGRGTLLFVDEIHRFNRSQQDGFLPVVENGTVTLIGATTENPSFALNGALLSRC
                     QVMVLRRLDDPALERLLTRSEAEIHRKLPVTEDARGTLRAMADGDGRYLLNMVEQIAA
                     LPADTPPMDTASLSELLARRAALYDKDREEHYNLISALHKSLRGSDPDAALYWFARML
                     NGGEDPRYIARRLVRFAAEDVGMADPSALPQAL"
     gene            complement(1051..1926)
                     /gene="bla"
                     /locus_tag="VGC82_17900"
     CDS             complement(1051..1926)
                     /gene="bla"
                     /locus_tag="VGC82_17900"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HEY0425197.1"
                     /translation="MIARRTMLAGSLFGAPALLSLRATAADDREGELRNRIADLERRH
                     GGRLGVAVLDTTTMKLFAYRGDERFPLCSTFKLLAAAFVLARVDSKQESLTRRVVYTK
                     DYLVTYSPVTEKHVGESGLTVGEICEAAVTLSDNTAGNLLLDSFGGPAGLTGYLRSIG
                     DNVTRLDRWETELNEARPDDLRDTTPPVAMLEVLHKTVLGTALSVSSREQLTAWLVAN
                     KTGDKRLRAGVPKGWRIGDKTGTGANNTTNDIAVIWSPEHAPIIVTAYYTGARGSDDE
                     RNAVLSEVGQLASAA"
     gene            complement(2154..3068)
                     /locus_tag="VGC82_17905"
     CDS             complement(2154..3068)
                     /locus_tag="VGC82_17905"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0425198.1"
                     /translation="MTKVMDAALVGANVVAKEEMMKALTQEQVAYYRDNGFLFPIPAL
                     TRPEVEFFLAGLQRLETELGGPVAEADVKWRSHAYAHSPWFDKLIRHPRILDAVEDVI
                     GPNILVWTSTFFIKEPHSKTFAAWHQDGTYFGLQPKEQVCAWVALTDATREAGCMEHL
                     SSHGSPRQLHHAPLGLKNSINRAGQTIMEPFDKSKPVAMALPAGSFSLHHELSVHRSA
                     PNHAAHRRVGIGLNYIPTHARVEGPVRCHAMLVRGEDTYGHFDLIDPPAAERDPVALA
                     VHQQVSDRYRANYNEQVRRHEQQFATTH"
     gene            complement(3141..3905)
                     /locus_tag="VGC82_17910"
     CDS             complement(3141..3905)
                     /locus_tag="VGC82_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY0425199.1"
                     /translation="MPAKNPTACLLVIGNEVLSGRTRDANIQFLATGLGNLGIPLREV
                     RVIPDVPETIIGTVNEVRAKFDYVFTTGGIGPTHDDITSECIAAAFGVPWEHHPVAWP
                     RMEASYKQGEFNAARQRMATMPRGAALIENAISVAPGFQMENVYVMAGVPRVMQSMFE
                     WLAPRLKGGAKIEMRAVHVVGLPEGLIAEGLAAVQQRYPDLDLGSYPFYRPTGNGVAL
                     VAKGPDGAEADRAIAEVTELIIGFGKVPIPGEPPEV"
     gene            complement(4059..5015)
                     /gene="mutT"
                     /locus_tag="VGC82_17915"
     CDS             complement(4059..5015)
                     /gene="mutT"
                     /locus_tag="VGC82_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEY0425200.1"
                     /translation="MTLRPLQPDDAEALHRLVNDWEVTRTLAEIPYPYPRALADDWIA
                     STAGELADGAAYHLAITGKEGRKETLVGIVGLRVDSSRRTGRLGYWVGRAYWKHGVAT
                     EAAKRLTSWGFANLPIDLITAEVTEGNDGSCKVLRRIGFREVGSGVRSSLTTRDEHAV
                     RVFEATRDDVFGPPDAPAADAANPDAKPLLLVAACALVDSDNRILLARRPEGKKMAGL
                     WEFPGGKLNPGETPEAALIRELKEELGIDVAAACLAPFAFASHDYEGFHLLMPLFVCR
                     RWKGTPKPREKQTLAWVRASKLTEYEMPPADKPLIPLLRDFL"
     gene            complement(5142..5324)
                     /locus_tag="VGC82_17920"
     CDS             complement(5142..5324)
                     /locus_tag="VGC82_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425201.1"
                     /translation="MVGPKPGLAPSRGLALGWAAFGARAVKSRIACIDRSGMSDVHRL
                     PLRPPHGLSHFAATPR"
     gene            5396..>5680
                     /locus_tag="VGC82_17925"
     CDS             5396..>5680
                     /locus_tag="VGC82_17925"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0425202.1"
                     /translation="MQLGIHLPHIGRKASPENIRRVAVQAEQLGLDDVWVSEHIIVPK
                     DCAYPPSPNFWDPVLTLTWAAAATSRVRLGTSVLVLPLRHPLPLAKELATL"
BASE COUNT         1015 a         1864 c         1763 g         1038 t
ORIGIN      
        1 agagcttgcg gaagcgcact gggatcggcc atgccgacat cctcggcggc gaagcgcacc
       61 aggcggcgtg cgatgtagcg cggatcctcg ccgccgttca gcatgcgggc gaaccagtaa
      121 agcgcagcgt cgggatccga cccgcgcagc gatttgtgca gtgccgagat caggttgtag
      181 tgctcttcgc gatccttgtc gtaaagcgcc gcgcgccgag ccaacagctc ggacaggctg
      241 gccgtgtcca tcggcggcgt gtcggcggga agtgctgcga tctgctcgac catgttcagc
      301 aaataacgcc cgtcgccgtc ggccatggcg cgcagtgtgc cgcgggcgtc ctcggtcacc
      361 ggcagcttgc ggtggatttc cgcctccgac cgggtcagca gccgttcgag ggcgggatcg
      421 tcgaggcgcc gcagcaccat gacctggcag cgcgacagca gcgcgccatt aagtgcgaag
      481 gagggatttt cggtcgtggc gccaatcagc gtgacggtgc cgttctccac cacgggcagg
      541 aaaccgtcct gctgggaccg gttgaagcgg tggatttcgt ccacgaacaa cagcgttccc
      601 cggcccgccc gccggcgttt cgtcgcatcg tcgaacgcgc gcttaaggtc ggcgacgccg
      661 gaaaacacag cggataactg aacgaacgcg agtttggccc gggatgccag cagacgggcg
      721 atggtggtct tgccgacacc cggtggcccc caaaggatca acgaagccag cgagcctcgc
      781 tccaacatgc gggtcagcga accctccggc ccaagcaaat ggtcctgccc aacgacttcg
      841 gacagcgtcg ccggccggag cctgtccgca agcggacggt tgatggcggg ttcgggtggc
      901 gccgccggtt cagcgtcgcc aaacaggtcg tcgggttctg gcacggccca actataacgg
      961 tgctgccgca gtcacgctaa tccggccgga tttcgatcta aaccctggct aaccaccggt
     1021 cagtcccgag caatcaccgg aacaatcggc tcatgccgcc gaggcaagtt gcccgacctc
     1081 cgacagaacc gcattccgct cgtcatccga cccgcgagct ccggtataat aggccgtcac
     1141 gataatcgga gcgtgctcgg gggaccagat gacggcaata tcgttcgtcg tattgttcgc
     1201 gccggtaccg gtcttgtctc cgatgcgcca acctttcggc acgccggccc gcaagcgctt
     1261 gtctccggtc ttgttggcga cgagccacgc ggtcagttgc tcacgagacg agacggatag
     1321 cgccgttcca aggaccgtct tgtgcagaac ttcaagcatc gcgaccggcg gcgtcgtgtc
     1381 gcgcagatca tcaggcctcg cctcgttcag ctcggtttcc cagcggtcga gccgggtaac
     1441 attgtcccca atcgaacgca aatagcccgt caaaccggct ggcccgccga agctgtcgag
     1501 cagcaggttg cccgcggtgt tgtcgctgag agttaccgcc gcttcgcaga tttcgccgac
     1561 ggtcaggccg ctctcgccca cgtgcttctc ggtaaccggg gaataggtga ccaggtaatc
     1621 cttcgtgtaa accacccgcc gcgtgaggct ctcctgtttg ctgtccaccc gcgccaggac
     1681 gaacgcggcg gcaagcaact tgaatgtgct gcacagggga aatcgctcgt caccgcgata
     1741 ggcgaacaac ttcatcgtcg tggtgtcgag aaccgcaact ccaagacggc cgccatgtcg
     1801 tcgttcgaga tcggcgatcc ggttccgcag ttcgccctcc cgatcatcgg cggcggtggc
     1861 gcgcaagctc aacagagccg gcgcgccaaa cagcgagccc gccagcatcg ttcggcgtgc
     1921 gatcaagatc gttctcctcg ggtgaattca gcgctaatcc tgtgacaggt ttccaccgtc
     1981 acgagaggac ttcgcgttct tctcatgcgc gggcttcagg aggtggcttt gtcatccgcc
     2041 gttcggaacg gcggacacat ccactcatcg gccacccgaa cggattgaag tccgccggcc
     2101 acagcgcgtt cttcatcggg ataccgccgc tgcggttcgg ttgtccttgc ccgtcagtgc
     2161 gtggtcgcga actgctgctc gtgacgcctg acctgttcgt tgtagttggc acggtatcga
     2221 tcgctcacct gctgatgcac agccagcgcg accgggtcgc gttcagccgc cggcgggtcg
     2281 atcaggtcga agtggccgta tgtatcctcg cccctgacca acattgcgtg gcagcggaca
     2341 ggcccctcca cgcgggcatg ggtcgggatg tagttcagtc cgattcccac gcgccggtgc
     2401 gctgcgtggt tgggggcgga gcggtgtacc gaaagctcgt gatgcagcga gaacgatccg
     2461 gccggcagcg ccatcgccac cggtttcgac ttgtcgaacg gctccatgat cgtctggccg
     2521 gcacgattga tgctgttctt caggcccagc ggcgcgtggt gcaactgcct cggtgagcca
     2581 tggctcgaca ggtgctccat gcagccggcc tcccgcgtcg catcggtcag ggcgacccag
     2641 gcgcagactt gttctttcgg ttgcagcccg aaataggtcc catcctggtg ccaggcggcg
     2701 aatgtcttcg aatgcggttc cttgatgaag aacgtgctgg tccacaccag gatgttgggg
     2761 ccgatcacgt cctcgacggc atccaggatc cgaggatggc ggatcaactt gtcgaaccaa
     2821 ggtgagtgcg cataggcgtg ggagcgccat ttgacatccg cctcggccac cggcccgccc
     2881 agttcggttt ccagccgctg caggccggca aggaagaatt caacctctgg ccgcgtcaac
     2941 gccgggatcg gaaacaggaa gccattatca cgataataag ccacctgctc ttgcgtcagt
     3001 gctttcatca tttcctcctt cgccaccaca ttcgcgccga ccagtgccgc gtccatgacc
     3061 ttggtcatca gaaccgacta cgggttgctc cgcaagttcg acgactgcca gttcgacgac
     3121 tgccaggccg gagcaccata tcaaacttcc ggcggctcgc cggggatcgg aactttgcca
     3181 aaaccgatga tcagttccgt tacctcggca atcgcccggt ccgcttcggc gccgtccgga
     3241 cccttggcca ccagtgcgac tccgttcccg gtgggtcggt agaacggata gctgcccaga
     3301 tccagatccg gataccgttg ctgcaccgcg gcgagcccct cggcgatcag tccttcgggc
     3361 agaccaacga catggaccgc gcgcatctcg atcttcgccc cgcccttcag cctcggggca
     3421 agccactcga acatcgattg catgacccgc ggcacgccag ccatcacata gacattttcc
     3481 atctggaaac cgggcgcgac gctgatggcg ttctcgatca gcgcggcgcc ccgaggcatg
     3541 gtcgccatcc gctgacgagc ggcattgaac tcgccttgct tgtagctggc ttccatgcgg
     3601 ggccaggcga ccggatggtg ctcccacggc accccaaagg ccgcggcgat acattcgctg
     3661 gtgatgtcgt cgtgcgtcgg accgatcccg ccagtggtga aaacgtagtc gaattttgcc
     3721 ctgacctcgt tcaccgtgcc gatgatcgtt tccggcacat cggggatcac ccggacttcc
     3781 cgcaacggaa tgcccaggtt tcccagaccg gtcgccagga actggatatt cgcatcgcgg
     3841 gtgcggccag acagcacctc gttgccgatg accagaagac aggcggttgg atttttcgcg
     3901 ggcatcgtcg ggttccctag agcatgatcg cgctgggcgg aacgtccggg cgcgtgaatc
     3961 atgcgatcaa gcaggttaaa tcatgatcgc gccgggcgga acgtccgggc gcgtcaatca
     4021 tgcgatcaag caagactaaa tcatgatcgc gctgggcatt acaggaagtc gcgcagcagc
     4081 gggatcaggg gtttgtcggc gggcggcatt tcatactccg tcagctttga cgcacgcacc
     4141 cacgccaggg tctgcttctc ccggggtttc ggggtgccct tccagcgccg gcaaacgaac
     4201 agcggcatga gcaggtgaaa cccctcatag tcgtgtgacg cgaacgcgaa aggtgccaga
     4261 caagcggccg ccacgtcgat accgagttct tccttcagct cccggatcaa cgccgcttcc
     4321 ggtgtttctc ccgggttgag cttgccgccc ggaaactccc acaggccggc catcttcttg
     4381 ccctccgggc gccgagccag caggattcgg ttgtcgctat cgaccagtgc gcatgccgcc
     4441 accagcagca acggtttggc atcggggttg gcggcgtcgg cggcaggcgc gtccggagga
     4501 ccgaaaacgt cgtcccgcgt tgcctcgaac accctgaccg catgttcgtc gcgggtcgtc
     4561 agggatgatc ggacgccgct gccgacctct cgaaagccga tccggcgcaa caccttgcac
     4621 gaaccgtcgt tgccctcggt cacttcggcg gtgatcaggt cgattggcag gttggcgaac
     4681 ccccagctcg tcagccgctt cgccgcctcc gtcgcgacgc catgcttcca gtaggcgcgg
     4741 ccaacccagt aaccaagccg tccggtgcgg cgagatgaat cgacacgaag gccgacgatg
     4801 ccaaccaggg tttccttccg gccctccttg ccggtgatgg cgagatggta agccgcgccg
     4861 tcggcaagct cgcccgccgt cgatgcgatc cagtcgtcgg ccagcgcccg aggataagga
     4921 tacggtatct ccgcaagcgt gcgcgtgacc tcccagtcgt tgaccaggcg gtgcaacgcc
     4981 tcggcatcgt ccggttgcag cggacgcagg gtcagccgct cggttttgag cgattcgaac
     5041 gcgggtcccg caaccgccgc tttaggtttg cgcgcggatt tccggggacg cttcaattgt
     5101 tgaatggttg aaatcggacg aaccttctca gggtggcctt gtcagcgtgg cgttgccgca
     5161 aagtggctca ggccgtgtgg cggacgcaag ggcaggcgat gcacgtccga catgcctgaa
     5221 cgatcgatgc aggcgatccg cgacttgacc gctcgggcgc cgaacgccgc ccagcccagc
     5281 gccaaccccc ggcttggggc cagccccggc ttggggccaa ccatcgacaa attccgggcc
     5341 caaccatgga caaattccgg gcgctgagga ctgatggcag caggaggaca cgatcatgca
     5401 gcttggcatc catctgcccc atatcggccg caaggcaagc cccgaaaaca tccgccgcgt
     5461 cgctgttcag gcggaacaac taggcctgga cgacgtctgg gtcagcgagc acatcatcgt
     5521 tccgaaggat tgcgcgtacc cgccctcacc caatttctgg gacccggtgc tgaccctgac
     5581 ctgggccgcg gctgcgacat cccgggttcg gctcggcacc tcggtcctgg tgctgccgct
     5641 gcgccatccg ctgccgctgg cgaaggaact cgccacactg
//
LOCUS       DASWQN010001049         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1156295, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001049 DASWQN010000000
VERSION     DASWQN010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156295"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VGC82_17930"
     CDS             <1..210
                     /locus_tag="VGC82_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425203.1"
                     /translation="DLVIGNDKTLRAVVSVGGFLGIGSKMVAVPFDKLQFGNTKGSND
                     NRVVIPGVTKDSLEGMPDYAYANRG"
     gene            complement(540..908)
                     /locus_tag="VGC82_17935"
     CDS             complement(540..908)
                     /locus_tag="VGC82_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY0425204.1"
                     /translation="MSTTTTAPRKTRALPPLMTLTDAAAERLRGLYAKGEQGKLLRIG
                     VKTKGCSGMSYDMSWTQEAAPTDEVVRDKDLTVLVDRKASLFLIGTVMDYEVKNLTAG
                     FTFTNPNEKGRCGCGESFHI"
     gene            complement(932..1303)
                     /locus_tag="VGC82_17940"
     CDS             complement(932..1303)
                     /locus_tag="VGC82_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEY0425205.1"
                     /translation="MNDVSPPATVWTPDGETPVETSGLPNEDRLIEAIATVYDPEIPV
                     NIYELGLIYAVEIDDDGGVKVEMTLTAPACPSAQELPEQVQNAVLTVPGVTSCHVETV
                     WDPPWDPSRMTEEARLQLNMF"
     gene            complement(1296..1787)
                     /locus_tag="VGC82_17945"
     CDS             complement(1296..1787)
                     /locus_tag="VGC82_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY0425206.1"
                     /translation="MTQTASTTTDGFDDLRDLYQEVILDHGRRPRHAVRLDDFDSTAK
                     GDNPMCGDRIQVWIKCNADAVDRVGFEARGCAISIASADLMVETVQGRGKADTRALFE
                     AFREMARTGACPSCDAALAEPIERLTPLAGVHEYPSRVKCATLPWHALIAALNGAKEA
                     SSE"
     gene            complement(1784..>2645)
                     /locus_tag="VGC82_17950"
     CDS             complement(1784..>2645)
                     /locus_tag="VGC82_17950"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY0425207.1"
                     /translation="EHHSNIIPWQLLRDAHGIEMRVAPVTDAGELDMAAFEALLEDGK
                     VGLVAITHMSNVLGTVVPAERVVQIAHAHGAKVLFDGSQAIVHRKVDVRALGCDFYAW
                     TGHKLYGPTGIGVLWGRRELLERMPPFMGGGDMISSVTYEQSTWAAVPHKFEAGTPAI
                     LEGIGLKAAIDYVQAIGYDAMAAHEASLTEHALARLASVEGLRILGQAQDRGGVISFV
                     MDKAHAHDVATLLDRQGIAVRAGHHCAEPLMHRFGLESTARASFGIYTTHDEIDALAD
                     GLARVREFFA"
BASE COUNT          510 a          859 c          758 g          518 t
ORIGIN      
        1 gatctggtta tcggcaacga caagacgctg cgcgccgtcg tttccgtggg cggattcctg
       61 ggaataggaa gcaagatggt cgccgtgccg ttcgacaagc tgcagttcgg aaacacgaag
      121 ggcagcaacg acaaccgcgt ggtcataccc ggcgtgacga aggactcgct cgagggcatg
      181 cccgactacg cgtacgccaa ccgcggttag gccgctgcgg gaggaacgtg atggcggtta
      241 cgaaccgcca ttcacgaact caatgccatt atcgcaggcc aaagaagctc agtacagcga
      301 tgaccaccac tacgagacca acgatataga tgatgttgtt catacaagcc tccctgtgac
      361 ggcgagatgc cagtcagaca ggcgtctaga accgcttagc ttgcttccgc gttgcttcaa
      421 aaaccggtgt cgcaccgaag tgcgacctct ttgtcacgct tcccgccacg gcaatggatg
      481 tctcgggacg atcaggcgat gaacggcgtc taaatttcat gcccgacagc ttttcggtat
      541 caaatgtgga aactttctcc gcagccgcag cggcccttct cgtttgggtt cgtgaaggtg
      601 aagccggcgg tcaggttctt gacctcatag tccatcaccg tgccgatcag gaacagcgac
      661 gccttgcggt ccaccagaac ggtcagatcc ttgtccctga caacctcatc cgtcggcgcg
      721 gcttcctgcg tccacgacat atcatacgac atgcctgaac accccttcgt cttcacgccg
      781 atgcgcagca gcttgccttg ttcacccttc gcgtaaagac cgcgcagcct ttcggccgcc
      841 gcatcggtca gcgtcatcaa gggcggcagt gccctggtct ttcgaggtgc ggtcgtcgtc
      901 gtgctcatgg gtcaacctat atcgtgatcg atcagaacat attcaattgc aaccgagctt
      961 cctcggtcat gcgggacggg tcccatggcg gatcccaaac ggtttccaca tggcacgagg
     1021 tgacgcccgg caccgtcagc acggcattct gtacttgctc cggcaactcc tgggcgctgg
     1081 ggcaagccgg tgcggtcaag gtcatttcca ccttcacgcc accgtcgtcg tcgatttcta
     1141 cagcgtagat caggccgagt tcgtagatgt tcaccgggat ttccggatca tacaccgtcg
     1201 cgatcgcctc gatcagccgg tcttcgttcg gcaggccgga tgtctcgacc ggtgtttcgc
     1261 cgtccggggt ccaaacagtg gcgggtggac tcacatcatt cactgcttgc ctccttcgcg
     1321 ccatttagcg cggcgatcag ggcatgccat ggtagcgtgg cgcacttgac cctggatgga
     1381 tattcatgga cgccagccag cggcgtcagg cgttcgatcg gttccgcgag tgccgcatcg
     1441 caagaagggc aggcaccggt gcgcgccatt tcgcgaaatg cctcaaacag cgccctggta
     1501 tccgcttttc cccggccttg caccgtttcc accatcaggt cggccgaagc gatgctaatc
     1561 gcacatcccc gcgcctcgaa cccgacacga tcgaccgcat cggcgttgca ttttatccag
     1621 acctggatgc gatcgccgca catagggttg tcgcccttgg cggtcgaatc gaagtcgtcc
     1681 agccgaaccg cgtgccgagg cctgcgacca tggtccagga tcacctcctg gtacaaatcg
     1741 cgcaggtcgt cgaaaccgtc ggtcgtcgtt gaagccgtct gggtcacgcg aagaactccc
     1801 gcacacgggc cagcccgtcc gccagcgcgt cgatctcgtc atgcgtggtg tagatgccga
     1861 agctggctcg ggcggtactt tccagaccga agcggtgcat cagcggttcg gcgcaatgat
     1921 gcccggcgcg aacggcaatt ccctggcggt ccagcaatgt cgccacatca tgcgcatgcg
     1981 ccttgtccat cacgaaggat atgacaccgc cgcgatcctg cgcctgaccc aggatccgca
     2041 acccctccac gctggccagc cgagccaacg catgttccgt gagcgaggcc tcatgcgcgg
     2101 ccatcgcgtc gtaaccgatc gcctgcacgt aatcgatggc ggctttcagg ccaatgcctt
     2161 ccaggatcgc cggcgttcca gcctcaaact tatgcggcac cgccgcccag gtggattgtt
     2221 cataagtaac cgaggaaatc atgtcgccgc cacccatgaa cggcggcatc cgctcgagca
     2281 gttcacgcct gccccacagc acgccgatcc cggtcggacc gtagagcttg tggccggtcc
     2341 aggcatagaa gtcgcacccg agggcgcgga catcgacctt gcggtgcacg atggcctgcg
     2401 acccgtcgaa caacaccttg gcgccatggg catgggcgat ctggaccaca cgttcggccg
     2461 ggacgacggt gcccagcacg ttcgacatgt gggtaatcgc caccagcccg acttttccgt
     2521 cttccagcag cgcttcgaac gccgccatgt cgagttcgcc cgcatcggtc acaggtgcga
     2581 cccgcatctc gatgccatgc gcatcccgca gcagctgcca gggaatgata ttggagtggt
     2641 gctcc
//
LOCUS       DASWQN010001050        10283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1949238, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001050 DASWQN010000000
VERSION     DASWQN010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 10283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10283
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949238"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VGC82_17955"
     CDS             complement(<1..355)
                     /locus_tag="VGC82_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425208.1"
                     /translation="MSDDPLDRLPSFDRVSIPAVLVHEGEDPGPALQEAGIVDAVALP
                     VIFDDDPHSPSGFFGDGITDNLSAVLEPAEVENDEDSPHASHAERPHQQPDGITSKAA
                     TVARLPAAFGSRPLAP"
     gene            complement(567..1055)
                     /gene="smpB"
                     /locus_tag="VGC82_17960"
     CDS             complement(567..1055)
                     /gene="smpB"
                     /locus_tag="VGC82_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY0425209.1"
                     /translation="MAEAKKKGMISHGIAAQNRKARFDYSIKDTTEAGMVLKGPEVKS
                     LRLGRATLTEAWAGEREGEMWLFNCYIPEYQGGVLSRFEPRAPRKLLLKKKQIVQLLS
                     EVSKQGATLVPLQIHFNDQGRAKVLLGVGLGRQKQDKRAAIADRDWKRDKARMMSTRG
                     KY"
     gene            complement(1149..2027)
                     /gene="dapA"
                     /locus_tag="VGC82_17965"
     CDS             complement(1149..2027)
                     /gene="dapA"
                     /locus_tag="VGC82_17965"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY0425210.1"
                     /translation="MFKGSLVALITPMREDGSIDEKAYAEFVDWQIKEGTNGVVPVGT
                     TGESPTMSHEEHRRAVEIAIEVSKGRVPVIAGAGSNSTEEAIALTRHAKEAGADAALI
                     VTPYYNKPTQEGMFLHFTAIADAVDLPIIIYNIPPRSVVDMSVETMARLAKHRNIIGV
                     KDATANLARPLHTRRGCGDDFCQLSGEDHTALSFNAAGGVGCISVTANVAPRLCGEMQ
                     AAWAEGRLKEAMAIQNRLVPLHDALFSETSPAPVKYAASLLGRTSERCRLPLAPIMEA
                     TRARVRAAMADVGLLN"
     gene            2211..4034
                     /locus_tag="VGC82_17970"
     CDS             2211..4034
                     /locus_tag="VGC82_17970"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0425211.1"
                     /translation="MNAVRAEHWADAQATAARYADPVAQKLVLYLRLRAQGAATSAEI
                     ADFMQRNPDWPAQVLLERRRQEAVASDPDDAAVLAQCSPPPTLAAAMLRCAEATANAG
                     RVGDANALARQAWVSGIDQAPGEAAFLRRWSGIATADDQWARFQRLAWTDAAAAGRQI
                     VHLDQAHHAAAEARLAAKRDDPQTEALVAALPAAFQADPGLILDRARALRRAERASEA
                     AALLARTGDTDNLPAFWSERNLLARKLLHDGDAKAGYAVVAVHGQLADEARAEAEFLA
                     GFIALRGLHDAPLAEQHFRTLVGSHAAITQSRAHYWLARSAAAAGKDARFEYEKSAGW
                     PTTFYGQLATLALGETPAGRIGKLRDPVWTPDNAIAFAGHEVMRAAAWLVAWGDPQRA
                     RGFLTRMDELAPIPAERALTAAFALKVGMPDGAVFVARRMGRDGIVLPHLGWPAPYDP
                     AQPPDAAFSLAVMRQESSFDVAAVSSSGARGLMQLMPPTATAVAKQLGIPASVPALTS
                     DANHNIRLGTAYLQEVLTRFDNCLPLAAAAYNAGPNRVAQWLSENGDPRAGPIDMVDW
                     IELIPVGETRNYVQRVSENVVVYRAARNDSAPILTTTPWTR"
     gene            4022..4855
                     /locus_tag="VGC82_17975"
     CDS             4022..4855
                     /locus_tag="VGC82_17975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0425212.1"
                     /translation="MDTISHRISAWDGLTLHVSEWFGGDRLAPILCLPGFVRTAADFE
                     TLAAAVGTGRRVVAIDYAGRGDSGRAGDVARYGPEACVRDVLDVCAALRIHHGVVIGT
                     SFGGLLAMGLAASRPGLVAAAVLNDIGPDVGSDGAAFVRDFVGRDPALESLDACVAFL
                     RAMLPPLSLDSDSAWRRMAELTYQPGADGRWHPVWDIRIARTLRHAPPDLWPLFGALA
                     HVPVLLVRGGVSNILLPATVARMQALRPDMRVVTLPGIGHAPILTEPPVQAAIQRFLE
                     T"
     gene            4852..5304
                     /locus_tag="VGC82_17980"
     CDS             4852..5304
                     /locus_tag="VGC82_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEY0425213.1"
                     /translation="MRLARFIAAGFGTGYAPVAAGTVASVAAVLVGAGLLDVSKPALA
                     LAALLATIGGYIAVERAEVTGDPGWVVIDEFAGQWITLLALPHPSVAGLAAAFVLFRL
                     FDITKWGPVGWADRRHGSFGIMADDVIAGLIAAALLLLIGAIRPQWLM"
     gene            complement(5319..6515)
                     /locus_tag="VGC82_17985"
     CDS             complement(5319..6515)
                     /locus_tag="VGC82_17985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0425214.1"
                     /translation="MITKFDSLFAGHVDIENIGYGGTPINDRRYDNAHLSTALSKAQS
                     IAQQMDQLGFSTFWMAEHHFQREGTECIPNVLLMALHLTHVTQNINIGCGFNIAPMWH
                     PLRLAEDYAMADILSKGRVVFGVGRGYHTREVETFGSPLMDQDANRELFEEQVDIIFK
                     AFNNDSFAHKGKHYTLPPEVPYRGYTLKELTLVPRPARLPVECWQPIQGGSERALAFM
                     AKHGIQGMVGGGSAEGGAMHDVVLKWQAEHARIGKQIELGERLCFGFHFYIGDNKEQC
                     IRDAAKYYEENMKMFGELRLNRAMTDEQIEIMRDPVRAPTAKLPRIEDAVKAGGFLTG
                     SPDEIIEHLKKLEKAYPGLDRISISPSLGVPEAVILEQLERFAKEVMPAFKEKQLEVK
                     APALAK"
     gene            complement(6634..7830)
                     /locus_tag="VGC82_17990"
     CDS             complement(6634..7830)
                     /locus_tag="VGC82_17990"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY0425215.1"
                     /translation="MNYHAAFAGMKVIDLSGGVAGPSCAMMLAQHGADVIKVETPHNG
                     GDWSRILGRTYQDHSAFSLYGTLGKRSLALDLKTAEGKEVLWRLVRGADVFIEGFRPG
                     TIQRYGFGYDAVSAREPGIIYYSISGFGQTGPLASRPAMDPVLQAFTGIVTENKGEHD
                     GHPHRIAISLIDMFSGLLGFQAIATSLYVRREQQVKQGRFIELSLMQGGAMLSVIRMI
                     AHHLERGTTQRTSMPNGVFNTSDGQINVTMIRPTDWPPFCEAIEQTQLLDDPRFATHL
                     ARASNLDELYEVLRPVLAARPTAWLAERLTARGIMNGRVNSYEEFLREEQVAATGIMS
                     WLTQPGVPEMVPMPNIPGLPPFENGTKRAHAPTLGEHTRDVLAEHGYSAAEIAALYEK
                     KVVAGG"
     gene            complement(7968..8750)
                     /locus_tag="VGC82_17995"
     CDS             complement(7968..8750)
                     /locus_tag="VGC82_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425216.1"
                     /translation="MQRLSPGFLVLSLIALWPAVVSPSPARADGGSACDIPADLTTPS
                     DALPAVKAALAAKRGLDVLAIGSGSTVGDAGASGGPALTFRSPEGSFPRKMVEALASI
                     RPALQVRLTVKGGRSMMAETMLPILQQELASHQYDLVLWQTGTVEAVHGIRPDALRAA
                     LQEGVDAAEKAHADLVLIDPQFSRFLRANADLSPYETVMQQITAGSEARLFHRFDLTQ
                     TWAGSGQIDVERVSREQRDKTIMLLNTCLGQALARFILTGAG"
     gene            complement(8755..9936)
                     /locus_tag="VGC82_18000"
     CDS             complement(8755..9936)
                     /locus_tag="VGC82_18000"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEY0425217.1"
                     /translation="MDLIRRSAARDLRVDFFRGLALWWIYTDHIPGDVLSDFSLRNFA
                     MCDATEVFVLLAGYGAGLSYGMGLGRNGYISTAADVLKRAWTLYVAHIFLFVLFTAQV
                     TYSATTLNRLNYLEETRLDVLGDDPYRSILEALLLRFQPSLLNILPLYVVLLLFFAAT
                     IWLLRWPRVLFGFSFAVYLVVRFTSLNLGSWGEDGWFFDPFAWQFLFIIGVLLACAPM
                     RPPRWPRLFDAASVMVSVAGVIVTTVIEPHPRLLDWVSASIFRSLVVEDKTGLYPFRL
                     ISILALTWLCVRLIRFDQAWLRSRWAAPLVLIGQNSLPVFCSGIVFGFLARLGLEHDD
                     GVLMQAGINLAGAIGMIAIGALAAWYRTKGKPNAPRGAGSHSEVRSGALLPVDAHPDT
                     G"
     gene            complement(10096..>10283)
                     /locus_tag="VGC82_18005"
     CDS             complement(10096..>10283)
                     /locus_tag="VGC82_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425218.1"
                     /translation="GKDFTISIRVSCNAQNVENMRDALSAYRDAGIQHVMAAPEDRDI
                     DTYLKTVEAFRRAGEGL"
BASE COUNT         1769 a         3355 c         3217 g         1942 t
ORIGIN      
        1 ccggggcgag tggccggctg ccgaaggccg cgggcagccg cgcgacggtg gcagccttcg
       61 atgttatccc atccggctgc tgatgcggcc gttcggcgtg ggatgcatgt ggggaatctt
      121 cgtcattctc cacctccgcg ggttccagca cggcagaaag gttatcggta atgccgtcgc
      181 cgaagaaacc gctgggggaa tgcgggtcat catcgaagat gactggaagg gctacggcgt
      241 ccacgatgcc cgcctcctgg agggccgggc cggggtcttc gccttcgtgg accagcactg
      301 ccgggatcga gacacggtcg aagctgggca gccggtccag gggatcatcg ctcatttggt
      361 aaggcccaga agttccttat ttgttcctac caaatttggt cagaatatca agcgccattc
      421 gcggcgggca tcggttgaac ggtttgcaat cagcgggatg ccgaccacga cgggctgagc
      481 cagatcaccc aactcgcggc agtcattatc aatagacgct tgtcaacgac atcgatcgga
      541 tcggtctggc gcggctggcc tgcggctcaa tacttaccgc gcgtgctcat catcctggct
      601 ttgtcgcgct tccagtcccg gtcggcaatt gccgcgcgct tgtcctgctt ttgccggcca
      661 agtccgaccc ccagcagcac ctttgcccgg ccctgatcgt tgaagtggat ctgcagcggc
      721 accaaggtcg cgccctgctt cgaaacctcg ctcagcaact ggacgatctg ctttttcttc
      781 agcagcaact tcctgggtgc ccgaggctcg aagcgcgaca gcacgccgcc ctgatattcg
      841 ggaatgtagc agttgaacag ccacatctcg ccttcgcgct cgcccgccca tgcctcggtc
      901 agagtcgccc gcccaaggcg cagcgacttc acctctggcc ccttcagcac cattccggct
      961 tccgttgtgt ccttgatgct gtagtcgaaa cgggctttgc ggttttgcgc ggcgatgccg
     1021 tgcgagatca tgcccttctt cttggcttca gccatggccg cttccgccaa gcccgagaac
     1081 ctcacgacga ggacttgatg aacctcctcc caccacggga accacggcgg ccgtccttgg
     1141 cgaaatcgtc agttcaacag accaacatcg gccatggcgg ccctcacccg agcgcgcgtc
     1201 gcttccatga tcggggccag cggcagccgg caccgttcgg aggtcctgcc cagcagggac
     1261 gcggcatact tcaccggtgc ggggctggtt tcgctgaaca aagcatcgtg gagcggcacc
     1321 agacggttct gaatcgccat cgcctccttc aaccggcctt cggcccacgc ggcctgcatt
     1381 tcgccgcata gccttggggc aacattcgcc gtcacgctga tgcagccgac gccgccggcg
     1441 gcgttgaacg acagagcagt atgatcctcg ccggacaatt ggcagaaatc gtcaccgcaa
     1501 ccacggcgcg tgtgcagcgg gcgagccaga ttcgcagtcg catccttcac gccgatgatg
     1561 ttgcggtgct tcgccagccg ggccatcgtt tccacgctca tgtccacgac gctgcgcggt
     1621 ggaatgttgt agatgatgat aggaaggtcc accgcatcgg cgatggcggt aaaatgcagg
     1681 aacatccctt cctgcgtcgg cttgttatag tacggtgtga cgatcagcgc tgcgtccgcg
     1741 ccggcttcct tggcgtgccg ggtcagggca attgcttctt ccgtgctgtt ggaacccgcc
     1801 ccggcgatca ccggcaccct gcccttggac acctcgatgg cgatctcgac cgcgcgacgg
     1861 tgttcctcgt gcgacatcgt cgggctttcg ccggtggtgc cgacaggaac gaccccattc
     1921 gtgccttcct tgatctgcca gtccacgaac tcggcgtagg ctttctcgtc gatcgaccca
     1981 tcttcccgca tgggtgtgat caacgcgacg agcgacccct tgaacatatc cttctcccga
     2041 ccggacttct gcctaaacgc gaaacctagg cgcgtgattg atgcgccgca aggctgggct
     2101 tgttattaga ggccatgcgc gttccgaaac gctttgtcct gacaggcttc gcccttgctg
     2161 gccttggcct gtcgccaacc tgggaagtcc gtgcccagga accaaacccc ttgaacgcgg
     2221 tccgggcgga gcattgggcc gatgcccagg cgacggcggc caggtacgcc gacccggttg
     2281 ctcaaaaact ggtgctctac ctgcgtcttc gtgcacaagg cgctgccacc tcggcggaga
     2341 tcgccgactt catgcagcgt aatccggact ggccggcgca ggtcttgctg gaacggcggc
     2401 gtcaggaggc cgttgcctcg gaccccgacg atgccgccgt cctggcacaa tgctcaccgc
     2461 cgccaacgct ggccgcagcc atgcttcgct gcgccgaggc caccgccaat gccggccgtg
     2521 ttggcgatgc caatgccctg gctcgccagg cctgggtcag cgggatagat caggcgcctg
     2581 gcgaggccgc ttttcttcgc cgatggagcg gcattgccac cgccgacgac cagtgggcgc
     2641 gttttcagcg tctggcctgg accgacgcgg cagctgcggg ccgacagatc gtccacctgg
     2701 atcaggctca ccacgccgcc gccgaagccc gtctcgccgc caagcgtgac gatcctcaga
     2761 ccgaggcgct ggtcgccgca cttcccgctg ctttccaagc cgacccaggt ctgatcctgg
     2821 accgcgcccg cgcccttcgc cgggccgaac gtgcgtctga agccgccgct ttgctcgctc
     2881 gaaccggaga tacggacaat cttccggctt tctggtcgga gcggaacctg ttggcgcgca
     2941 agctgctgca tgacggcgac gccaaggctg gttatgccgt ggttgcggtg catggccaac
     3001 tcgccgacga agcgcgcgcc gaggctgaat ttcttgccgg cttcatcgcc ctgcgcgggc
     3061 tgcacgacgc gccgctggcg gaacagcatt ttcgaacgct cgttggttcc catgccgcga
     3121 tcacgcaatc ccgagcgcat tattggcttg cgcgttcggc cgccgcggcg ggaaaggatg
     3181 ctcggttcga gtatgaaaag tccgccgggt ggcccacgac cttctatgga cagttggcca
     3241 cgctggcgct gggcgaaact cccgccggtc ggatcggcaa gctgcgtgac ccggtctgga
     3301 cgccggataa tgccatagcc tttgccggcc acgaggtgat gcgcgccgcc gcctggctgg
     3361 tcgcctgggg tgacccgcag cgcgcgcgcg ggttcctgac gcggatggac gaactcgcgc
     3421 cgataccggc cgaacgagcc ttgaccgccg ctttcgcgct gaaagtcggg atgcccgatg
     3481 gcgccgtgtt cgtcgcgcgc cgcatgggac gcgacggaat cgtgctgccg catctgggct
     3541 ggcctgcgcc atacgatccg gcccaaccgc ccgatgcggc tttctcgctg gcggtcatgc
     3601 ggcaggagag cagcttcgac gttgctgccg tcagttcttc cggcgcccgc gggctgatgc
     3661 agttgatgcc gccgacggcc acggcggtcg cgaaacaact tggcatcccc gcttcggtgc
     3721 cggcgttgac atccgatgcg aatcacaaca tccgcctcgg gaccgcctat ctgcaagagg
     3781 tcctgacgcg cttcgacaat tgcctgccgc tggcggcggc ggcctataac gccggtccga
     3841 accgggttgc tcaatggcta agcgaaaatg gcgatccgcg cgcgggcccc atcgacatgg
     3901 tcgattggat cgaactgatc ccggttggcg aaacccgcaa ctacgtccag cgggtcagcg
     3961 aaaacgtggt ggtgtatcgg gcggcgcgca acgattccgc accaattctg acgaccacac
     4021 catggacacg ataagccatc gcatcagcgc ctgggatggc ctgacgctcc acgtcagcga
     4081 atggttcggc ggcgaccgcc tggcgccgat cctctgcctt cccggtttcg ttcgtaccgc
     4141 cgcagacttc gagacactgg ccgcggcggt cggcaccggc cgccgtgtcg tcgcgatcga
     4201 ctatgccggc cgaggtgact cgggccgtgc cggcgacgta gcccgttacg gccccgaagc
     4261 ctgtgtccgg gatgtcctcg atgtctgcgc cgccttgcgc atccaccacg gcgtggtcat
     4321 cggcacgagc tttggcggcc ttctcgccat gggtctcgcc gcttctcgtc ccggcttggt
     4381 cgccgccgcg gtgctgaacg acatcggtcc ggacgtaggg tcggacggcg cggcgttcgt
     4441 gcgggatttc gttggcaggg acccggcgct ggaaagcctg gacgcctgcg tcgcgtttct
     4501 acgggcgatg ctgccgccgc tgtcgctgga ttcggattcc gcatggcgcc ggatggcgga
     4561 actgacctac cagcctggcg cggacggacg ctggcacccg gtctgggaca ttcggatcgc
     4621 aaggaccctg cgacacgcgc ctcccgatct ttggccgctg tttggggcgc tggctcatgt
     4681 gccggtcttg ctggtgcgcg gcggggtcag caacatcctt ttgccggcta ccgtggcccg
     4741 gatgcaggcg ctccgtcccg acatgagggt cgtaacgctg cccggcatcg gccatgcacc
     4801 gattttgacg gagccgcccg tgcaggctgc gatccagcga tttctggaaa catgaggctt
     4861 gcccggttta tcgcggccgg attcggcaca ggttatgcgc ccgtcgccgc cggaacggtc
     4921 gcctccgtgg ccgcggtgct ggtgggtgcc ggattgctgg acgtgtccaa gccggcgctg
     4981 gcgctggctg cattgctcgc gacgatcggc ggctatatcg cggtggaacg cgccgaagtg
     5041 accggcgacc ccggctgggt cgtgatcgac gagttcgccg gccagtggat cacgcttctc
     5101 gcgttgccgc atccgtcggt cgccggtctg gccgccgctt tcgtcttgtt caggcttttc
     5161 gacatcacca agtggggccc ggtcggttgg gccgaccgcc ggcatggcag cttcggtatc
     5221 atggccgacg acgtcatcgc cggccttatc gcggcggcac ttttgctatt gataggtgcg
     5281 atacgtccgc aatggctgat gtaggacgga cttggacgct acttggccag agccggagct
     5341 tttacttcca actgcttttc cttgaacgcc ggcatcactt ccttggcaaa gcgctccaat
     5401 tgttccagaa tcacggcctc gggcacgccc agcgaagggc tgatcgagat ccgatccaga
     5461 cctggatagg ctttttcgag tttcttcaga tgctcgatga tctcatcggg cgagcctgtc
     5521 aggaagccgc cagccttcac cgcgtcttcg atccgcggca acttcgcagt gggcgccctc
     5581 accggatcgc gcatgatctc gatctgctca tcggtcatcg ctcggttcag gcgcagttcg
     5641 ccgaacatct tcatgttctc ttcgtagtat ttggcggcgt cacggatgca ttgttctttg
     5701 ttgtcgccga tatagaaatg gaatccgaag cacaggcgct cgccgagctc gatttgcttg
     5761 ccgatcctgg catgttccgc ctgccatttc agcacgacgt catgcatcgc gccgccctcg
     5821 gccgaaccac cgcccaccat gccctgaatg ccgtgcttcg ccatgaacgc gagcgcgcgc
     5881 tccgacccgc cttggatcgg ttgccagcat tccaccggca gccgggccgg gcgcggcacc
     5941 agagtgagct ccttcaacgt gtagccgcga tacggcactt ccggcggcag ggtgtagtgc
     6001 ttgcccttgt gcgcgaatga atcgttgttg aaagctttga agatgatatc gacctgttcc
     6061 tcgaacagtt cacggttggc atcctgatcc atcaacggag agccgaaggt ttcgacctcc
     6121 cgcgtgtggt agccacgacc gacgccgaac acgacgcggc ccttcgacag gatgtcggcc
     6181 atggcgtaat cctcggccag gcgcagcggg tgccacatcg gcgcgatgtt gaagccgcag
     6241 ccgatgttga tgttctgggt gacatgcgtc aggtgcagcg ccatgagcag gacgttcggg
     6301 atgcactcgg tgccttcgcg ctggaagtgg tgctccgcca tccagaatgt ggaaaaaccg
     6361 agctggtcca tctgttgggc gatggactgc gctttcgaca gcgcggtgga gagatgggcg
     6421 ttgtcgtagc gccggtcgtt gatcggggtg ccgccgtagc cgatattctc gatatcgaca
     6481 tggcccgcga aaaggctgtc gaatttggtg atcatggccg ttgttcctcc gatattgcct
     6541 cgatagtacg cttgcgcgcg gccttggcaa gcgggaccgt cgctataagg cggcgggaaa
     6601 ctgttccgac agacgaggcc gcgccgctgt aattcagccg ccggcgacca ctttcttctc
     6661 gtacagcgct gcgatctccg ccgccgaata gccatgctcc gccagcacat cccgcgtgtg
     6721 ctcgcccagc gtcggggcat gggcccgctt ggtgccgttc tcgaacggtg gcagccccgg
     6781 aatattcggc atcggcacca tctctggcac gcccggttgg gtcagccagg acatgattcc
     6841 ggtcgctgcc acttgttcct cgcgcaggaa ttcttcgtag ctgttgaccc ggccgttcat
     6901 gattccgcgc gccgtcagcc gttcggccag ccatgcggtc ggccgggccg ccagcaccgg
     6961 ccgcagcacc tcgtaaagct cgtcgaggtt gctggcgcgt gcgagatggg tggcgaaccg
     7021 cgggtcgtcc agcaactgcg tttgttctat cgcctcgcag aatggcggcc agtcggtcgg
     7081 acgtatcatg gtgacgttga tctggccgtc cgaggtgttg aacaccccgt tcggcatgct
     7141 ggtgcgctgc gtcgtcccgc gctccagatg atgcgcgatc atccggatca ccgacagcat
     7201 cgcaccgcct tgcatcaggc taagctcgat gaaccggcct tgcttcacct gctgctcgcg
     7261 acgcacataa agcgatgtcg cgatggcctg aaacccgagc aggccggaga acatgtcgat
     7321 cagcgagatc gcgatgcggt gcgggtggcc gtcatgctcg cccttgttct cggtcacgat
     7381 cccggtgaac gcctgcagca ccgggtccat cgccggacgt gacgccaatg gtcctgtctg
     7441 gccgaagccg gagatcgaat agtagataat cccgggctcc ctggcgctga ccgcgtcgta
     7501 cccgaagccg tagcgctgga tggtgcccgg cctgaagccc tcgatgaaca catcggcacc
     7561 gcgcaccaag cgccagagga cctcttttcc ctccgccgtc ttcaggtcca acgccaggct
     7621 gcgtttcccg agcgtgccat acagcgagaa ggcggagtgg tcctggtagg tccggcccag
     7681 gatgcgggac cagtcgccgc cgttgtgcgg cgtctccacc ttgatcacgt ccgcgccgtg
     7741 ctgggccagc atcatggcgc aggacggtcc ggcaaccccg ccgctaaggt cgatcacttt
     7801 catgccggcg aaggctgcgt ggtagttcat cgtttccccc ggttatcgtg gcgcggcggg
     7861 atgataaggg gccattcgat ccggaggcta cccctggcgc cctcctggcg ccccctcctt
     7921 ggcacccagg cacgagatca ccggccgaac gccggcgtcg ttcacttcta tccggcaccc
     7981 gtcagaatga agcgggcgag cgcctgaccc aggcaggtgt tcaacagcat gatcgtcttg
     8041 tcccgctgct cgcggctgac ccgttccacg tcgatctgac ctgatccagc ccatgtctgg
     8101 gtcaggtcga agcggtgaaa caacctcgct tccgacccgg cggttatttg ctgcatcacc
     8161 gtctcatagg ggctgaggtc ggcgttggcg cgaagaaacc ggctgaactg cgggtcgatc
     8221 agcaccaggt cggcgtgcgc cttttctgcc gcgtccacgc cctcctgcaa ggcagcccgc
     8281 aaggcgtccg ggcgtatgcc gtggacggcc tccaccgtgc cggtctgcca caggacaagg
     8341 tcgtattgat gcgacgcgag ttcctgctgc aggatcggca gcatcgtctc ggccatcatg
     8401 ctgcggccgc ccttgacggt cagcctgacc tggagcgccg ggcggatcga cgccagcgct
     8461 tccaccatct tgcgcgggaa cgaaccctcg ggtgagcgaa acgtcaacgc cgggccaccc
     8521 gatgcgcctg cgtcaccgac cgtggacccg gagccgattg ccaggacatc cagcccgcgt
     8581 ttggctgcca gcgccgcctt taccgcaggc agcgcatccg aaggcgtggt gaggtcggca
     8641 ggtatgtcgc acgcggatcc gccatccgcc cgggccgggg aagggctcac cacggccggc
     8701 cacaaagcga tcagcgacag cacaagaaag ccaggcgaaa gccgttgcat gacgtcaccc
     8761 ggtatcaggg tgcgcatcta ccggcaagag cgccccggaa cgtacctcgg aatgcgatcc
     8821 tgcgccgcgc ggcgcatttg gcttgccttt ggtgcgatac caagcggcca aagcaccgat
     8881 ggcgatcatg ccgatcgctc cggccagatt gattccggcc tgcattaaca caccatcgtc
     8941 atgctccaac ccgaggcgtg cgaggaaacc gaagacgatc ccggagcaga acaccggcaa
     9001 ggagttctgc ccgatcagca ccaggggtgc ggcccagcgc gaccgcagcc aagcctgatc
     9061 gaagcgaatc agccggacgc acagccaggt gagcgccagt atcgagatca gccggaacgg
     9121 atacagcccc gtcttgtcct ccacgaccag cgaccggaag atggaagccg atacccaatc
     9181 gagcaggcgg ggatgcggct cgatcaccgt tgtcacaatg actccggcaa cggaaaccat
     9241 gaccgaagcg gcatcgaaca atcgtggcca ccttggtggc cgcatcggcg cgcaggccag
     9301 cagaacgccg atgatgaaca ggaactgcca agcgaatggg tcgaagaacc agccgtcctc
     9361 accccaggag cccagattaa ggctggtgaa ccgcaccacg agatagaccg cgaacgagaa
     9421 gccgaacagg acgcgcggcc accgcaacag ccagatcgtg gcagcgaaaa acaacagcag
     9481 caccacgtaa agcggcaaga tattcagcag gctgggttgg aaccgcagca acagggcttc
     9541 caggatggac cggtacggat cgtcgcccag cacgtccagc cgggtttctt ccagatagtt
     9601 cagccggttc agcgtggtgg ctgaataagt cacctgggcg gtgaacagca cgaacagaaa
     9661 gatatgggca acatacagcg tccaggcgcg cttcaacacg tccgcggctg tcgagatata
     9721 gccgttgcgg ccaagcccca ttccatagga caggccggca ccgtagcccg cgagcagcac
     9781 gaagacctcg gtcgcgtcgc acatggcgaa attgcgcagg ctgaaatcgc tcagaacatc
     9841 gccggggatg tgatccgtat agatccacca cagcgcgagg ccgcgaaaaa aatcaacccg
     9901 aagatcacgt gccgcggatc gcctgatcag gtccattacg ctgatgtggt aaaggccggc
     9961 ggaaccgaaa tcacggcgat gcgatccctt gcatcacgtt ttcgaggttg atcttattcg
    10021 ttcggggccg tttggtgcag tgcgtggtcg ccgccgaacc ggcacgttcg ccggaaaggt
    10081 ctggatgcgc tgctgctaaa gcccttcccc ggcgcgccgg aatgcctcga ccgtcttgag
    10141 gtaggtgtcg atgtcgcggt cttctggcgc tgccatgacg tgctggatgc cggcatccct
    10201 gtaggcgctc aacgcgtccc gcatgttttc cacgttttgt gcgttgcaac tgacgcggat
    10261 cgaaattgtg aagtccttac cag
//
LOCUS       DASWQN010001051         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1949306, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001051 DASWQN010000000
VERSION     DASWQN010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949306"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3050
                     /locus_tag="VGC82_18010"
     CDS             <1..3050
                     /locus_tag="VGC82_18010"
                     /inference="COORDINATES: protein motif:HMM:NF009588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY0425219.1"
                     /translation="SGDALHCANMGGTHRHGGVLAVSGDDHGAHSSTYPHQTEYVFQN
                     CFIPVLNPGSVQDVIDLGLAGWAMSRFAGVWVAMKTTAETMEQAATVVVPSRRSFVTP
                     DIALPPHGLNFDHTMHFPADRAELERRMIEERMPAVLAWARVNRIDRLISGSADASIG
                     LVTVGKAHEDTLHALRRMGIDQHQQLAVYKIGMTWPLETEGLRNFARGKRFILVIEEK
                     RSFVESQIRDALYHLPADERPEVSGKTDPRGGALLSPLMELSPESVAVGLAKFLSQAG
                     LNLPAPSAPVAIERPGGLLRRVPAFCAGCPHATSTKLPDGSFASAGIGCHFMAMDDGP
                     FTRTFTHMGGEGAPFVGLSSFTDVKHMFANLGDGTYQHSGSLAIRQAVAAKTRITYKL
                     LFNDAVAMTGGQPTEGQPTVPMIAAQLTAEGVKRIAIVADEADRLPPASALPPGVTRH
                     TREDLDAVQKSLRDFDGPSVLIYDQVCATEKRRRRKRGSMVQPARHVVINQAVCENCG
                     DCSVQSACIAIEPVETEFGRKRRINPTSCNVDLSCLKGFCPSFVTQAGPPAAPEADMQ
                     WQARETELAATLPDPVVPSPAVWRGLFAGIGGGGIVTSGAILAMAAHLEGRAVKTLDF
                     TGMAQKNGAVVAHVQIAADEAALDVVRIPIGSADLMLAADLAVSCQAGVLERNARSAV
                     VIGNMDLAATADFKRDATLSLDAALHRRTIEKVTDAGRSTWLSGVRLAERLFGNAQAM
                     NTLLLGIAWQRALIPVGQTAILRAIELNGAAVKLNKRAFLWGRILAERPELMKQILDG
                     LTDGPPADLDALIAHRAAALTSYQSKRYARRFQILVRNVVERETTVFGAPGRLSRAAA
                     EGLYRVMAYKDEYEVARLHAAATYGEQPEFHMSPPLVAGIDEATGRRRKIAIPGWLGL
                     PLFRIMRYGKIVRGSPLDPFGYQAERRHERALMAQYTADLRAVLSALRADTLEVSVAV
                     AELPDQIRGFGPVKDANRATAASRRAQLLAQLAHPEPVAVAAE"
BASE COUNT          516 a          981 c         1037 g          522 t
ORIGIN      
        1 gttccggcga cgccttgcat tgcgccaaca tgggcgggac ccacaggcat ggtggcgtcc
       61 tggcggtttc cggcgacgac cacggggcac actcgtcaac ctatccgcac cagaccgaat
      121 atgttttcca gaattgcttc atcccggtcc tgaatcccgg ttcggtccag gatgtgatcg
      181 acctcggcct ggcggggtgg gcgatgtccc gctttgccgg cgtctgggtc gccatgaaga
      241 caaccgccga gaccatggag caggccgcca cggtcgtcgt tccctcgcgc cgaagcttcg
      301 tcacccccga catcgcgctg ccgccgcacg ggttgaattt cgaccacacc atgcattttc
      361 cagccgatcg cgcggaactc gagcggcgga tgatcgagga gcgcatgccg gcggtactcg
      421 cctgggctcg ggtcaaccgc atcgaccgct tgatcagcgg atcggccgac gccagcatcg
      481 gcctggtcac ggtcggcaag gcgcacgagg acacgttgca tgcgctgcgg cggatgggga
      541 tagaccagca ccagcaactc gcggtgtaca aaatcggcat gacctggcca ctggaaaccg
      601 aagggctgcg gaatttcgcc cgcggcaagc gattcattct ggtcattgag gaaaagcgga
      661 gtttcgtgga gagccagatc cgcgacgcgc tgtaccacct gccagcggac gaacggccgg
      721 aggtcagcgg caaaaccgac cctcgtggcg gcgcgctgct gtcgccgctg atggagctgt
      781 cgccggagag cgtagccgtg ggtttggcga agtttctcag tcaggccggg ctgaacctgc
      841 cggcaccgtc ggcgccggtc gccatcgaac ggccgggcgg gttgttgcgc cgcgtgccgg
      901 cgttctgcgc cggctgcccg cacgcgacct cgacgaagct gccggacgga agtttcgcca
      961 gcgccggtat cggctgccac ttcatggcga tggacgacgg cccgtttacc cgaaccttca
     1021 cccatatggg cggcgagggc gcaccctttg tcggcttgtc cagctttacc gacgtgaagc
     1081 acatgttcgc caatctggga gatgggacct accagcactc cggcagcctg gcgatccgcc
     1141 aggcggtcgc ggcgaaaacc cgaattactt acaagctgct gttcaacgac gccgtcgcga
     1201 tgaccggcgg ccagccgacc gagggccaac ccaccgtccc gatgatcgcc gcgcagctca
     1261 cggccgaagg cgtcaagcga attgccatcg ttgccgacga ggccgaccgt ctgccgccgg
     1321 cgtccgctct gccgcccggt gtcacgcgcc acacgcggga ggacctggac gcggttcaga
     1381 agtccctgcg cgacttcgat ggtccgtccg tgctgatcta cgaccaggtt tgtgccactg
     1441 aaaagcgccg ccgccgtaag cgggggtcga tggttcagcc ggctcgccac gtcgtcatca
     1501 accaagccgt ctgtgagaac tgcggcgact gctcggtgca gtccgcgtgc atcgcgatcg
     1561 agccggtgga aaccgaattt ggccgcaagc ggcggatcaa cccgacaagc tgcaacgtcg
     1621 atctgtcctg cctgaaaggt ttttgcccaa gcttcgtcac ccaggccggc ccgcccgcgg
     1681 cgccggaggc ggacatgcaa tggcaggcgc gggaaacgga gctggcagcg actctgccgg
     1741 atccggtcgt tccgtcgcca gcggtgtggc gcgggctgtt cgccggcatc ggcggcggcg
     1801 ggatcgtcac ctccggggcg atcctggcga tggcggcgca tctcgaagga cgcgcggtca
     1861 aaacgctgga tttcactggg atggcgcaga aaaacggtgc tgttgtcgcg catgtgcaga
     1921 tcgcggcgga tgaggccgcc ctggatgtcg tccgtatccc gatcggcagc gcggacctga
     1981 tgctggcggc ggacctcgcg gtcagttgcc aagccggcgt gctggaacgg aacgccagat
     2041 cggccgtggt gatcggcaat atggatctgg cggcaacggc ggatttcaag cgcgacgcga
     2101 cgctgtcgct ggatgccgcg ctgcaccggc gcaccatcga gaaggtgacg gacgccggcc
     2161 ggtcaacctg gttgtctggg gtccggcttg cggaacgtct gtttggcaat gcccaggcga
     2221 tgaacacgct gctgctgggg atcgcctggc agcgtgctct gattccggtc ggacaaaccg
     2281 cgatcctgcg cgccatcgaa ctcaacggcg ccgcggtgaa gcttaacaag cgggcgttcc
     2341 tctggggccg catcctggcg gagcggcccg agctgatgaa gcaaatcctc gatggcctga
     2401 ctgatggtcc tccggccgat ctggacgcgt tgatcgcgca tcgggcagcg gcgctgacgt
     2461 cgtatcagtc gaaacggtat gcgaggcggt ttcagatatt ggttcgaaac gtggttgaac
     2521 gcgaaaccac ggtgttcggt gcgccaggcc gtctttcgcg cgccgcggcc gagggcctgt
     2581 atcgcgtcat ggcctacaag gacgagtacg aggtcgctcg gctgcatgcc gccgcgactt
     2641 atggcgagca gcccgaattc cacatgtcgc cgccgttggt cgccggtatc gacgaggcaa
     2701 ccggccggcg tcgcaagatc gccatcccgg gctggctggg actgcctctg ttccggatca
     2761 tgcgctacgg caagatcgtt cgcggctcgc cgttggaccc cttcggctat caggcggagc
     2821 gtcggcacga aagggcgctg atggcgcaat acaccgccga cctgcgcgcc gtgctgtccg
     2881 cactgcgggc ggacactttg gaagtttcgg tcgcggtggc ggaactgccg gatcagattc
     2941 gcggcttcgg gccggtgaag gacgccaatc gggcaaccgc ggcgtcacgg cgggcgcaat
     3001 tgctggcgca actcgcacat cccgagcccg tcgcggtggc ggccgaatag cgcctt
//
LOCUS       DASWQN010001052         4673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_893893, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001052 DASWQN010000000
VERSION     DASWQN010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893893"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..468)
                     /locus_tag="VGC82_18015"
     CDS             complement(295..468)
                     /locus_tag="VGC82_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425220.1"
                     /translation="MPKAPVSGCPRRGHRQGLGSVKKILDSLTFANNPRAHFSRQYLP
                     CTSELMKGLVHML"
     gene            complement(623..1285)
                     /locus_tag="VGC82_18020"
     CDS             complement(623..1285)
                     /locus_tag="VGC82_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY0425221.1"
                     /translation="MAVRTLLLDLDGTLVDTVPDLAAALNRLMRGRGLPAFSHKQVAA
                     MVGDGVAALAARAFAAHDRQADASAVAELTADYTAHVAVDSKPYRGVLPVLTGLARDG
                     WLLAVCTNKPEQAARRLLQAVGLLPLLCAVGGGDSYAFRKPDPAHLLATLMRAAGRPE
                     SAVMLGDHRNDIVAARDAGLPSIFAAWGYGGAAMAEGSTGVASDIAEAAAIANRLLPL
                     KE"
     gene            1492..2808
                     /gene="glmU"
                     /locus_tag="VGC82_18025"
     CDS             1492..2808
                     /gene="glmU"
                     /locus_tag="VGC82_18025"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY0425222.1"
                     /translation="MQATAIILAAGLGSRMKSSLPKTLHRLAGRSMLRHLLASCEPVF
                     DRIVVVLGPDMDAVRKEAEPHICVVQHERLGTAHAALQAAEHFGSGQVAVLYADNPLI
                     RTATLRRLLETHPDRGLCLLAFRPADPARYGRVITGAGGLVERIVEYADASLAERAVN
                     LCNAGVLCGGATEMRGWLQAVRNDNAKEEYYLTDVVALARADGVPVSAVEAPADELAG
                     INSRSELARAEAVLQGWMREAAMAAGVTMIDPSSVFLSADTRLSADVTIEPNVFFGPN
                     VTVASNVIIRAFSHIEGATIGPGSIVGPFARLRPGAELERDVHIGNFVEIKASRLASG
                     VKASHLSYIGDATVGTGTNIGAGTITCNYDGTSKHRTTIGAHVFVGSDVALVAPVSVG
                     DGAVIAAGSVITEDVEPDALALARGRQVQKPGRAIEMRAAAGKGKN"
     gene            2809..4632
                     /gene="glmS"
                     /locus_tag="VGC82_18030"
     CDS             2809..4632
                     /gene="glmS"
                     /locus_tag="VGC82_18030"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY0425223.1"
                     /translation="MCGIVGVIGARPAAPILLDALRRLEYRGYDSAGIATLVNGHIER
                     RRAEGKLGNLALSLEQAALVGTIGIGHTRWATHGAPTESNAHPHGTARVSLVHNGIIE
                     NHAELRAELEAEGQEFSTETDTETVAQLVDLNLKRGMAPIEAAGAALRRLEGAYALAM
                     IFAGHPELIVGAQHGAPLAVGFGDDEMFVGSDALALAPLTRRIAYLRDGDWTVVDRHG
                     AEFFDSSGTKVEREVKLTRLTGAAIGKANFRHFMEKELHEHPAVIGDTLHQMVSPGSR
                     AVALPDLGIDFRKVPRITMSACGSAFYAGLVGRWWFEAIARIPTDGDVASEFRYRTPP
                     LPKGGLGLLVSQSGETADTLAALRYMREQGQQVLSILNVPESTMARESDAVLETVAGP
                     EIGVASTKAFTAQLSVLACLALAAGRATGAISATQEAAMTAALLEVPSRAAEVLDNWD
                     AIQSVALRVSRARDVLFLGRGNCFPIALEGALKLKEISYIHAEGYAAGEMKHGPIALI
                     DPAVPVIAIAPSGPLFEKTASNLQEAAARGGQVIVFSDAAGAAKLKGIAAETVILPAV
                     DPFVAPILYAIPVQMLAYQVAVLKGTDVDQPRNLAKSVTVE"
BASE COUNT          873 a         1485 c         1542 g          773 t
ORIGIN      
        1 cggctaattc agtattgtcc ctgtcctgcg gttaccataa acaagttctt aaatatccag
       61 cgtcggtcgt gacccgcgtc gtgaaattcg caaccacatc cagttcttag ttgcctgatc
      121 attgatgccg gactgaccaa gaacccccaa acccaccagt cgatccaccc aagaggatca
      181 aggtggtttc tctgcattat tcgtgctgcc gcgacccacc gtccattccg gagaatgccg
      241 atgtcacgca aaaaggacct gggcgaacca agccctgagg aactgatcga agcgttacag
      301 catgtgaacg aggcccttca tcagctcgga ggtgcatggc aaatactgcc gggaaaagtg
      361 cgcgcgcgga ttgttggcga aagtcaaaga atcgaggatc ttcttgaccg agccgaggcc
      421 ttgccggtgc cccctccgcg ggcaccccga gaccggcgcc ttcggcatct gccagttcct
      481 aagcctgtct catcaaggac gactcaggtt ctgggacgca aatatcgtaa ggctcggtcg
      541 ccgactccgg cttcgaacga tcttccgaca gcgacctgac tcggtaatcg gaacgctcca
      601 gcctatcggc cgcaagtgat ccctactctt tgagcggcaa caaccgattg gcgatagcgg
      661 cggcctcggc gatgtcgctt gccacccccg tactgccctc cgccatcgcg gctccgccat
      721 aaccccaggc ggcgaaaatg ctcggcaaac ctgcatcccg cgccgctaca atatcgttgc
      781 gatgatcgcc cagcattacc gccgactcgg gtcgcccggc tgcccgcata agcgtggcaa
      841 gcaggtgcgc ggggtccggt ttgcggaagg cataactgtc gccaccgcca accgcgcaca
      901 gcaacggcag tagcccaacc gcctgaagca accgccgcgc cgcctgctcg ggcttgttgg
      961 tgcacaccgc cagaagccag ccgtcgcggg ccaatccggt cagcaccggc agcacgcccc
     1021 ggtaaggctt gctatcgaca gcaacatgcg ccgtgtagtc ggcggtaagt tcggcaaccg
     1081 cgctggcgtc agcctgccgg tcatgcgcgg cgaaggctcg cgccgcgagg gccgccacgc
     1141 cgtcgccaac catcgcggcc acctgtttgt gggagaacgc gggcaacccg cgcccgcgca
     1201 tcagccggtt cagcgccgcc gcgaggtccg gcacggtatc caccagcgtc ccgtccagat
     1261 cgagcagcag tgtccgaacc gccatgttgc actccgaaac gcgaattgaa ccaatagcat
     1321 aagagagact ttgacatccc tccacggaca ccgctacgcc agacgaattc gtggtcggtc
     1381 ctggccttgg gaaccaagcg tcaggggaat cggcgcacca aatcgacgct tggtgaacaa
     1441 atctgtcgga cctgccgcca gatccagccg ccagcgaagc agggaccgag catgcaagca
     1501 accgcgatca tcctggccgc cggcttgggc agccggatga agtcgtcgct gccaaaaacg
     1561 cttcaccgtc tggccggtcg gtcgatgctg cggcatctgc tggcaagctg cgaaccggtt
     1621 ttcgaccgta tcgtcgtcgt actgggtccc gatatggacg ccgtgcggaa agaggccgaa
     1681 ccgcatattt gcgtagtcca gcacgagcgc ctgggcacgg cgcatgccgc gctacaggcc
     1741 gccgaacatt tcggcagcgg ccaggtcgcg gtgctctacg ccgacaatcc gctgatccgg
     1801 acagcgacgc tgcggcggct gctcgagacc caccctgatc gaggactatg cctgctggcg
     1861 tttcgccccg ccgatccggc gcgctatggg cgggtgatca ccggtgctgg aggcttggtg
     1921 gagcgcatcg tcgaatacgc cgacgcgtcg ctggccgaac gggcggtgaa cctgtgcaat
     1981 gcgggcgtgt tgtgcggtgg cgcgaccgag atgcgcggat ggctgcaggc ggtgcgcaac
     2041 gataacgcga aggaagaata ctaccttacg gatgtcgtcg cccttgcgcg cgccgatggc
     2101 gtgccggtgt cagcggtcga agcacccgcg gatgaactgg ccggcataaa ctcccgcagt
     2161 gaactggctc gggccgaggc ggtactgcaa ggctggatgc gcgaggcagc gatggccgcc
     2221 ggcgtgacga tgatcgatcc gtcctcggtc ttcctatcgg ccgacacgcg gctatcggcg
     2281 gacgtcacca tcgagccgaa cgtgtttttc gggccgaacg tgaccgtcgc gagcaatgtg
     2341 atcatccgcg ccttcagcca tatcgaaggg gccactatcg gtcccggcag cattgtcggc
     2401 ccgttcgccc gcctgcgacc gggtgcggaa ctcgaaaggg acgtgcatat cggtaatttt
     2461 gtggagatca aggcttctcg tctcgcatcc ggtgtgaaag ccagccatct tagttatatc
     2521 ggtgacgcca cggtcggaac cggcaccaat atcggagcgg ggacgatcac ctgcaattac
     2581 gacggcacca gcaagcatcg gaccacgatc ggcgcgcatg tcttcgtcgg ttcggacgtt
     2641 gccttggtgg cgccggtttc cgtcggcgac ggcgcggtga tcgccgctgg cagtgtgatc
     2701 accgaagacg tggaaccgga cgctctggcc ctggcgcgcg ggcgccaggt gcagaaaccc
     2761 gggcgggcga tcgagatgcg ggcggcggcg ggaaaaggaa aaaactgaat gtgtggcatt
     2821 gtcggcgtca tcggggcccg tcccgcggcg cccatcctcc tggatgcgct tcggcggctg
     2881 gaatatcgcg gctatgacag cgcgggaatc gccacgctgg tgaatggcca catcgaacgg
     2941 cgccgcgcgg aaggcaaact ggggaacctt gccctgtcgc tggaacaggc cgcgctggtc
     3001 ggcaccatcg gcattggcca cacgagatgg gccacccacg gggcgcccac cgagagcaat
     3061 gcccatcccc acggcaccgc tcgcgtatcg ttggtgcata acgggatcat cgaaaaccat
     3121 gccgaactgc gggccgaact ggaagccgag ggccaggagt tcagcaccga gaccgatacc
     3181 gagaccgtcg cccagcttgt cgatctgaac ctgaagcgtg gcatggcgcc gatcgaggcg
     3241 gccggcgccg cgctacgccg actcgaggga gcctacgccc tggcgatgat cttcgctggt
     3301 catccggaac tgatcgtcgg cgctcagcat ggcgcaccac tggcggtcgg cttcggcgac
     3361 gacgagatgt tcgtcgggtc cgacgcgctg gcgctggccc cgctgacccg ccggattgcc
     3421 tacctgcgcg atggcgattg gacggtcgtg gatcgccacg gggcggaatt cttcgacagc
     3481 agcggcacca aggtggagcg cgaggtcaag ctcacccggc tgaccggggc agcgatcggc
     3541 aaagccaact tccgccactt catggaaaag gagctgcacg agcaccccgc ggtgatcggc
     3601 gacacgctgc accagatggt cagccctggc agccgtgcgg tggcactgcc ggacctgggg
     3661 atcgactttc gcaaagtgcc gcggatcacc atgagtgcct gcggcagcgc cttctatgcc
     3721 ggcctggttg ggcgctggtg gttcgaggcg atcgcccgaa tccccaccga cggtgatgtc
     3781 gcgagcgagt tccgctaccg cacgccaccg ctgccgaagg gtggcctcgg gctgctggtg
     3841 tcgcaatcgg gcgaaacggc ggatacgctg gcggcgttgc gctacatgcg cgagcagggc
     3901 cagcaggtgc tttccatcct caatgtccct gaaagcacca tggctcggga gagcgacgcg
     3961 gtgctggaaa ccgtcgcagg gccggagatc ggggtggcaa gcaccaaagc gttcaccgct
     4021 cagctttcgg tgctggcgtg tttggcgctg gcggcgggcc gcgccacggg ggcgatcagc
     4081 gcgacccagg aagcggcgat gacggcggcg ttgctggagg tgcccagccg agcggccgag
     4141 gtgctggaca actgggacgc gatccagtcg gtcgcgctgc gcgtctcgag agccagggac
     4201 gtgctgttcc tgggccgcgg caactgtttc ccgattgccc tggagggcgc gctgaagctg
     4261 aaggaaatca gctacatcca tgccgaaggc tacgcggctg gcgagatgaa gcatgggccg
     4321 atcgcgctga tcgatccggc ggtgccggtg atcgccatcg cgccgtccgg gccgctgttc
     4381 gagaagaccg cgtcgaacct gcaggaggca gcggcgcgcg gaggccaggt gatcgtgttc
     4441 agcgacgcgg cgggtgcggc gaagctgaag ggcatcgcgg cggagacagt gatcctgccg
     4501 gcggtcgatc cgttcgtggc gccgattctg tacgcgatcc cggtgcagat gctggcctat
     4561 caggtcgcgg tgctgaaagg taccgacgtg gaccagcccc ggaacctcgc gaagtcggtg
     4621 acggtggagt aaggccgcac cggtaacata gaacgcggca accgcggtga ttt
//
LOCUS       DASWQN010001053         4493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1817758, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001053 DASWQN010000000
VERSION     DASWQN010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817758"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VGC82_18035"
     CDS             complement(<1..243)
                     /locus_tag="VGC82_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY0425224.1"
                     /translation="MTGTKPRVGLFVTCLVDLHRPSIGFAAIRLLERAGCQVEVPRAQ
                     TCCGQPAYNSGDRRTARDLAAGIVEAFRGYDYVVVPS"
     gene            complement(281..952)
                     /locus_tag="VGC82_18040"
     CDS             complement(281..952)
                     /locus_tag="VGC82_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004449012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEY0425225.1"
                     /translation="MGASGGFPIDLVLFGMIAAFLVLRLRSILGKRTGLERQAPPVQP
                     APVRPAAGPIIDAKVEPQAAAAPQTVPEPTSRSGQALARMREQDPNFDPARFLAGAEQ
                     AFKMILGAFAAGDRVALRPLLADETYAAFEQAISAREKAGETQVSEVKSMQHLSIDDA
                     ELKGRVGSVTARIVSDQISYTKDKNGRPLTGTDAVTEITDLWTFERDLSQPDPTWRLV
                     AARSG"
     gene            1085..1639
                     /locus_tag="VGC82_18045"
     CDS             1085..1639
                     /locus_tag="VGC82_18045"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0425226.1"
                     /translation="MILLRHGQSEFNLHFGATRRDPGIIDPRLTELGHAQAEEAAKQL
                     AGQGIKRIIVSPYTRTLQTARPVAAALGVPVVVDPIVRERYAFACDVGTPRPALERDW
                     PAHDFGLIEEIWWPAEEEPETSVVGRAAQFRSGLAERHDWANTLVVSHWGFILSLTGQ
                     SVANGQWLRCDPTAAAPERLVWRA"
     gene            1758..2291
                     /gene="secB"
                     /locus_tag="VGC82_18050"
     CDS             1758..2291
                     /gene="secB"
                     /locus_tag="VGC82_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY0425227.1"
                     /translation="MSETTPSPQAAGPTPTQAAPAPLVVNIQYVKDLSFEVPGAPQIY
                     SQLKAQPQVSINLDVQARRVQEGQSVYEVSIIIRAEAHDMAGQANGEAVNPAPTVFVA
                     ELTYAGVFTLNNLPENAVEPVLLVECPRILFPFARNILSDVTRDGGFPPVLLQPIDFV
                     ALWQSRRAQAGAVTAAA"
     gene            2599..3321
                     /gene="phbB"
                     /locus_tag="VGC82_18055"
     CDS             2599..3321
                     /gene="phbB"
                     /locus_tag="VGC82_18055"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY0425228.1"
                     /translation="MARIALVTGGTRGIGAAISLALKAQGRSVVANYASNDEAAEAFS
                     REHGIPVAKFNVSSFEECEAGVGKVTAEHGPIEILVNNAGITRDGTLLRMRRDMWDAV
                     LDTNLGSCYNLCKLTFEGMRDRKFGRIVNVGSVNGQAGQYGQVNYAAAKSGIHGFTKA
                     LAQEGARYGITVNAIAPGYVDTEMVRAVPGEVLRKIVAKIPMGRLGHAEDIARGVAFL
                     TADDADFVTGSTLSINGGMHMY"
     gene            3415..4290
                     /locus_tag="VGC82_18060"
     CDS             3415..4290
                     /locus_tag="VGC82_18060"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0425229.1"
                     /translation="MNAPRNTQYRHLDVQKIAGSLGAEVRGVDLSQDVSEEVLAEIRA
                     ALLDNLVIAIRDQDLTPAQQLAFARRWGDIHLHPFMEGMADYPEILAIIKRPTDKKNF
                     GGSWHTDQMFSPSPAMGTMLYAKEVPSAGGDTLFTNQYLAYESLSDGMKAIAGQLRTV
                     CVGDNFKAAGGLSRAQRYDRQMSEMKVKDPGDVVTTSVHPLVRTHPETGRKSIYIGGH
                     VQHFDGMTDEESQPLIDFFMRQSIRPEHTCRVRWQTGTLTFWDNRCTQHFAVNDYPAE
                     TRIMHRITVCGDQPF"
BASE COUNT          862 a         1454 c         1383 g          794 t
ORIGIN      
        1 ggacggcacg accacgtaat cgtatccccg gaacgcctcg acgatccctg cggcgaggtc
       61 gcgcgcggtc cgccggtccc ccgagttata cgccggttgc ccgcagcagg tctgcgcccg
      121 gggcacctcc acctggcatc ccgcgcgctc cagcagcctg atcgccgcga acccgatcga
      181 aggccggtgc agatcgacca ggcacgtcac gaacagacca acccgaggct ttgtcccggt
      241 catcgaaacc ccagcatcaa atgcctcaat tgccgccccg tcagccactg cgcgcggcca
      301 ccaaacgcca ggtcggatcc ggttgcgaca gatcgcgctc gaacgtccaa agatcggtga
      361 tttccgtcac cgcgtcggtg ccggtcagcg gccgcccgtt cttgtccttg gtgtagctga
      421 tctggtccga cacaatccga gccgtgaccg atccgacgcg tcctttgagc tccgcgtcgt
      481 cgatcgacag gtgctgcatc gacttgacct cgctcacctg agtctcaccc gccttttccc
      541 gggcgctgat cgcctgctcg aacgccgcgt aggtttcatc cgccagcaac ggccgcaatg
      601 ccacccggtc gcccgcagca aacgcaccca ggatcatctt gaacgcctgt tcggcgccgg
      661 ccagaaagcg agcggggtca aaattcggat cctgctcccg cattcgcgcc agggcttgac
      721 cgctacggga tgtcggttcg ggaacggtct gcggtgcggc tgccgcttgc ggctcgacct
      781 tggcgtcgat gatcgggccg gccgccggtc gcacaggcgc gggttgcacc ggcggcgcct
      841 gccgctcgag ccccgtacgc ttgcccagga tactgcgcag ccgcagcacc aaaaatgccg
      901 cgatcatacc aaacagcacc agatcgatcg gaaatccgcc ggacgcaccc atgccacacc
      961 ctcctcagta ttcaacagat aggcgcgagc atggggcatg acaaacgaaa ccgggccacg
     1021 ggcgcttgca gacgagcctt ttgacggcct tgatcggcca acactgtcac tatatggccg
     1081 tcgcatgatc ctgctgcgcc atggccagag cgagttcaac ctgcatttcg gcgccactcg
     1141 ccgcgatcct ggcatcatcg atccgcgcct gaccgaactc ggtcatgccc aggcggagga
     1201 agccgcgaaa caattggcag gacagggaat caagcggatc atcgtgtccc cctacaccag
     1261 gacgctacaa actgcccggc cggttgccgc tgcgcttggc gttcctgttg tggtcgatcc
     1321 gatcgtgcgg gagcgatatg cctttgcgtg cgacgtcggc actccccgtc ctgccctcga
     1381 acgcgactgg cccgcgcacg actttgggct gatcgaggaa atctggtggc cggccgagga
     1441 agagcctgag acatccgtgg tcggccgcgc ggcccagttc cgcagcggtc ttgccgaacg
     1501 ccacgattgg gcgaacacgc tggttgtcag ccattggggt ttcatcctta gcctgacggg
     1561 acaaagtgtc gccaacggcc aatggctgcg ctgtgacccg accgcagccg cgccggagcg
     1621 attggtctgg cgcgcatagg cccagcggcg ccgaaacctg ttggcaggcc ggtaacaatc
     1681 atgctacccg gcccgcggtg atacgccgca ggcgacgcga tgcttaggcg acgcgatcct
     1741 taaagaacgg agccatgatg tccgagacta caccttcccc acaggccgcc ggcccgacac
     1801 ccacgcaagc ggcacccgcg ccgctggtgg tcaacatcca gtatgtgaag gacctttcgt
     1861 tcgaggtccc cggtgcgccc cagatctaca gccagttgaa ggcgcaaccg caggtcagca
     1921 tcaacctgga tgtccaagcc cgccgcgtgc aggaaggcca gagcgtttac gaggtttcga
     1981 tcatcatccg agccgaggct catgatatgg ccggccaagc caatggcgag gccgtcaacc
     2041 cggcaccgac cgtgttcgtc gccgaactga cctatgccgg cgttttcacc ctgaacaacc
     2101 tgccggaaaa tgcggtcgaa ccggtgctgc tggtcgaatg cccgcgcatc ctgttcccat
     2161 ttgcccgcaa catcctgtcc gacgtgacgc gcgatggcgg cttcccgccg gtgctgctgc
     2221 agccgatcga cttcgtcgca ctgtggcagt cgcgtcgcgc ccaggccggt gccgtcaccg
     2281 ccgccgcatg atggcgagct gctgacttgt tgtgcggccg ggttggttga tcgagagtcc
     2341 ggctgaataa gtgcccgatg ataaatgacc ggttccaacc gggcgaactt ccccagcaac
     2401 gtcatggtgg gcctttgtcc accatgacgt tatccgagac gcgcccgaag cggttgcttc
     2461 ggagcggacc tcacgagggt tactcgaatc cggttgtaga aacccggaaa aagcgccggt
     2521 tccgcggtcc cgcacccttt gcagattcgg cggaagtgct aagagtacca accatctgaa
     2581 caacaacggg agagaccgat ggcacgcatc gcacttgtta ccggcggcac tcggggtatt
     2641 ggcgccgcta tcagcctggc gctgaaggcg cagggccggt cggttgtcgc gaattatgcg
     2701 tccaatgacg aagcggccga ggcgttctcg agggaacacg gcatcccggt tgcgaagttc
     2761 aatgtcagca gcttcgagga atgcgaggcc ggcgtcggga aagtcaccgc cgaacacggg
     2821 ccgatcgaga tcctggtgaa caatgccggc atcacccggg acggcacgct gctgcggatg
     2881 cgccgggaca tgtgggacgc ggtgctcgat accaatctcg ggtcctgcta caacctgtgc
     2941 aagctgacgt tcgagggcat gcgcgaccgc aagtttggcc gcatcgtcaa tgtcggcagc
     3001 gtcaacggcc aggccggaca atacggccag gtcaactatg ccgccgccaa gtcggggatt
     3061 cacggcttta ccaaggcgct ggcccaggaa ggtgcgcgct acggcatcac ggtcaacgcg
     3121 atcgcccccg gttacgtcga taccgagatg gtgcgggcgg tgccgggtga agtattgcgc
     3181 aagatcgtgg caaagatccc gatgggacga ctgggacatg ccgaggacat tgcccgcggc
     3241 gttgcgttcc tgaccgccga cgacgccgat ttcgttactg gttcgacgct gtcgatcaat
     3301 ggcggcatgc atatgtattg agcgcgatcc ggggtgggcc aaggcccacc tggtttcctt
     3361 gccatgggtc cagaccaggg gcattatttc ggcacgacag gaggattccg gccaatgaac
     3421 gcaccgcgca acacgcaata ccgccatctg gacgtccaaa agatcgccgg ttcactcggc
     3481 gccgaagtac gcggggttga tctgtcgcag gacgtatcgg aggaggtgct ggccgaaatc
     3541 cgagcggcgt tgctcgacaa tctggtgatc gcgatccggg accaggacct aacgccggcc
     3601 cagcagcttg ccttcgccag gcgctggggg gacatccact tgcacccctt catggagggc
     3661 atggcggatt acccggaaat cctggcgatc atcaaacgcc caactgacaa gaagaacttc
     3721 ggcggatcat ggcataccga ccagatgttc tcgcccagcc ccgcgatggg cacgatgctt
     3781 tacgccaagg aagtgccgtc cgccggcggc gacacgttgt tcaccaacca gtatctggcc
     3841 tacgagagcc tgtcggacgg catgaaggcg attgccgggc agctccgcac ggtctgcgtc
     3901 ggcgacaatt tcaaggcagc cggcggcctg tcccgtgcgc aacgctacga ccgccaaatg
     3961 agcgagatga aggtcaagga tccgggcgat gtggtgacga ccagcgttca tccgctggtt
     4021 cgcacgcatc ccgagaccgg ccggaaatcg atctacatcg gcggccatgt ccagcatttc
     4081 gacggcatga ccgacgagga gagccagccg ctgatcgact tcttcatgcg gcaatcgatc
     4141 cgtccggaac atacctgccg cgtgcgctgg cagaccggga cgctgacgtt ctgggacaat
     4201 cgttgcaccc agcatttcgc ggtgaacgat tatccggcgg aaacccggat catgcaccgg
     4261 attacggtat gcggggatca gcctttctga cttgattcat ctccgtcggg cgtgaatggc
     4321 acgttcaggt cgtccgttcg ctcgtcgggc cggcttgccc gacgcttgcc tgttgccgga
     4381 tccgctcgat cagatcggcg ccaggttcag gttggatgaa catccagcca gccaaatgcc
     4441 agtagtggcg tctgcactga ataacgatga tgcgttgatc gacgtcaggc ttg
//
LOCUS       DASWQN010001054         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1685219, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001054 DASWQN010000000
VERSION     DASWQN010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685219"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VGC82_18065"
     CDS             complement(<1..200)
                     /locus_tag="VGC82_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425230.1"
                     /translation="MRARLSGISRDLAGLDDHSRFDRLLGFDKLLADRFTPINGRGTP
                     EDVGRLKMWGERPWRRVTGRSQP"
     gene            complement(249..1649)
                     /locus_tag="VGC82_18070"
     CDS             complement(249..1649)
                     /locus_tag="VGC82_18070"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEY0425231.1"
                     /translation="MTAPPDLSKLSDADKDRLILALIAQVAAAQATIATQARRIAALE
                     ARLDELTRPPKTPDNSSKPPSQGQKQDRPAPGTDRPPRKSRPGVGRMLHPNPDRVVEA
                     RLSACPKCQAMFPDAAQTPQQVYERIELPPIKPDVTQVRLFGGRCACCGERVTAEAPA
                     GLEPGSPFGRSIAAMVVYLHDAHAIGMERLATLMDELVSLSLSEGAISNILGRARAPL
                     LAAAATIQAVVLTSPVVCSDETSTRVKGKTWWEWVFVGTLAVLHVIQPSRGKAVVRAL
                     FGAIRPGVWVSDMLGSQRGHGVLWQVCLAHLLRDAKYAVECGDSAFSAPFRRLLLRAV
                     AIGRRRETLKDTTLKRYLGDLDRRLDRIMAAVPIGEPGRKLRKRMAANRAHLFVFMIN
                     RDVPYTNNVSERHLRPSVIFRKVTNGFRCEWGSETYAAFRSVVGTAKLNRASVFDTIQ
                     FVLSAKSSVEALAAAG"
BASE COUNT          371 a          657 c          628 g          394 t
ORIGIN      
        1 ggttgtgaac ggccggtcac ccgccgccat ggtcgctcgc cccacatctt caggcgccct
       61 acatcttcag gggttccccg cccgttgatc ggggtgaacc tatccgccag caacttgtcg
      121 aagcccaaca acctgtcgaa gcgtgagtga tcatcgagac ctgcgagatc acggctgatg
      181 ccggacagcc gtgcgcgcac tacagcccgg gccgttgtgt ggcgattctg ctattcgctg
      241 gtaattgctc accccgccgc ggccaatgcc tccacggatg attttgcgga cagcacgaac
      301 tggatggtat cgaacaccga agcccgattg agcttcgcgg tgccgacgac ggagcggaag
      361 gcggcgtagg tctccgatcc ccactcgcag cggaacccgt tggtcacctt gcggaagatc
      421 acgctggggc ggagatgccg ctccgacacg ttgttggtgt acggcacgtc ccggttgatc
      481 atgaacacga acagatgcgc ccggttggcg gccatgcgct tgcgcagctt gcggcctggt
      541 tccccgatgg gaaccgctgc catgatgcga tccagccgtc gatccagatc gccaagatat
      601 cgcttcaggg tggtgtcctt cagcgtctct cgccgtcgtc cgatcgcaac agcgcgcaac
      661 aacagccgcc ggaacggcgc gctgaacgcg ctatcgccgc attcgacagc atatttcgca
      721 tcgcgcagca ggtgcgccag gcacacctgc cacagaacgc catggccacg ttggctgccg
      781 agcatgtcgg acacccagac cccgggcctg attgcgccga acagcgcccg caccacggcc
      841 ttgccgcggc taggttgaat gacgtgcagc acggccagcg tgccgacaaa cacccattcc
      901 caccaggtct tgcccttgac ccgcgtcgag gtctcatcgg aacagaccac cgggctggtt
      961 agcaccaccg cttggatcgt cgccgccgca gccagcagcg gggcgcgggc gcgtcccagg
     1021 atattgctga tcgccccctc gctgagggac agagagacga gttcgtccat caacgtggcc
     1081 agccgttcca tgccgatggc atgggcgtca tgcagataga ccaccatcgc ggcgatagag
     1141 cggccgaacg gcgaacccgg ctctaatccg gcaggtgcct ctgccgtcac ccgctcgccg
     1201 caacaggcgc agcgaccgcc gaacaggcgc acctgggtca cgtccggctt gatcggcggc
     1261 agttcgatgc gttcgtagac ctgctgcggg gtctgcgcgg cgtccgggaa catcgcctgg
     1321 cacttcgggc aggccgacag cctggcttcg accacacggt cggggttcgg gtgcagcatc
     1381 cggccaacgc cgggccggct cttgcggggt ggccgatccg tgccgggcgc gggacggtct
     1441 tgcttctggc cctgcgacgg cggcttggac gagttgtcgg gggtctttgg tggacgggtc
     1501 agttcgtcga gccgcgcctc cagcgccgcg atacggcgcg cctgcgtcgc gatggtcgcc
     1561 tgcgccgccg ccacctgcgc gatcagggcc aggatcaacc gatccttgtc ggcatcgctg
     1621 agcttgctga ggtcaggtgg tgcggtcact cagcatttag accgaacttc ctgcttgcca
     1681 atactgtaaa tggttgatct gaaagggtga aatccaatca gagggagccg ctactggcta
     1741 catgttaccg ggcattggaa aagcgtggcc ttttcgcgaa accgcctgac gtagctgagc
     1801 atattgccgt cgcccggaat actcacgatc cgtttattta catggacaca tttttcaggc
     1861 tttttgacca aaaattgtgc gttatcaagg ctctaaagta attcccagaa ggaagttcgg
     1921 gctaaaggac agcgctggga acatccctaa tgctattcct gtatgctttc ctcaatctgc
     1981 cggcagtaca tgcgatggtc agcagcccgt tgttggtccc ccctcgcttc tctggaaatg
     2041 tgttgagctt
//
LOCUS       DASWQN010001055         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1552767, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001055 DASWQN010000000
VERSION     DASWQN010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552767"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VGC82_18075"
     CDS             complement(<1..673)
                     /locus_tag="VGC82_18075"
                     /inference="COORDINATES: protein motif:HMM:NF025694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate cyclase domain-containing protein"
                     /protein_id="HEY0425232.1"
                     /translation="MRALIDRIEDLIHRDVGRGMDAVFQATRGGLWDAASALAACSAP
                     KVGLITGFYVPGGDPPAAETDGPAAAALLALGFVNAGLSCRLATDTLCQRACRVALDA
                     AGAGGVAVDAAPPGDTVDRLIERWREDGIDWVIAIERCGPGADSRPRNMRGTDISAHV
                     APLDRLFSAGPWRTIGIGDGGNEIGMGRVPRSLIAQHVPLGEMIGCVVPADFLIAAGV
                     SHWGAY"
     gene            complement(747..1547)
                     /locus_tag="VGC82_18080"
     CDS             complement(747..1547)
                     /locus_tag="VGC82_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HEY0425233.1"
                     /translation="MNAITDVRGNAGERERLRIRTGAFTGPTSGLAPGNVQANLVILP
                     KALAHDFLRFAQANPKPCPVLAVSEAGDPHFPTLGAELDIRTDLPRYRVWSDGKLISE
                     PTDVRDVWRDDLVSFAIGCSFSFEDALIEDGIEVRHIARGCNVPMYRTNIPCVSAGIF
                     SGPLVVSMRPLKPADAIRAVQITSRFPSVHGAPVHLGFPASIGIADIAKPDYGDAVPI
                     AADEMPVFWACGVTPQAVIAQVRPEFCITHAPGSMLITDLRNTSLAAL"
     gene            1673..2206
                     /locus_tag="VGC82_18085"
     CDS             1673..2206
                     /locus_tag="VGC82_18085"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0425234.1"
                     /translation="MSSPAFQLGKIGHVALYVADVKRSTQFYTDVLGFHVSDAYEDDM
                     MPGGAAFLRCNPDHHGIALFKATEQNPAGAGLHHIAFEVPTLDDVVRARAHLRKHDVQ
                     IDFDGRRRAGVQIAVEFRDPDNHRLEIYWGIDQIGPGEQARPAHEWKGALSLEAAIAD
                     PVVGQDTSLKDPSLLET"
     gene            complement(2321..>2652)
                     /locus_tag="VGC82_18090"
     CDS             complement(2321..>2652)
                     /locus_tag="VGC82_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425235.1"
                     /translation="SSGGKQVGGRHACQAGDRARCSLCVAGTCRGWALFGLYDNAKTL
                     RTSSAARPPPSWRPLCRLAAAFKRFNKVVGGRAKHGHDTMINLRPRSPSVACDHQPAH
                     TIMRRRR"
BASE COUNT          485 a          907 c          851 g          409 t
ORIGIN      
        1 cataggcgcc ccaatgcgag acccccgccg cgatcagaaa atccgccggc accacgcagc
       61 cgatcatctc gcccagcggc acatgctgcg cgatcagcga tcgcgggacc cgacccatgc
      121 cgatttcatt gccgccgtcg ccgattccga tggtccgcca cggaccggcg ctgaacaacc
      181 ggtccagcgg cgcgacgtgg gcagagatgt cggtgccgcg catgttccgc ggacgcgagt
      241 cggcccctgg accgcaacgc tcgatggcga ttacccagtc gatgccgtct tcgcgccagc
      301 gctcgatcag cctgtccacg gtgtcccccg gaggcgcggc atcgacggca acgccgccgg
      361 cgccggccgc atccagcgcg acacggcaag cccgctggca aagcgtatcg gtcgccagcc
      421 ggcacgacaa accggcgttg acaaaaccga gcgccagcag ggcggcggcg gcgggcccgt
      481 cggtttcggc ggccggggga tcgccgccag ggacatagaa gccggtgatc aggccaacct
      541 tcggtgccga acacgcagcc agggcggacg ccgcgtccca caggccgcct cgggtcgcct
      601 ggaagacggc gtccatcccc cgcccgacgt cacggtgaat caggtcctcg atccggtcga
      661 tcagcgcccg catgaccgtc gatgcggcaa atggcgcgcc cagtcgagac tacgccggcc
      721 gctcatggcg cggcagcaac cagcacctac agcgccgcca gggaggtgtt ccgcaggtcg
      781 gtgatcagca tgctgcccgg tgcgtgtgtg atgcagaact cgggccgcac ctgcgcgatc
      841 accgcctgag gcgtaacgcc gcaggcccag aacaccggca tctcatcggc tgcgatcggt
      901 acggcgtcgc catagtcggg cttcgcgatg tcggcgatcc caatggatgc cgggaacccc
      961 agatgcaccg gcgcgccgtg caccgacggg aaacgcgacg tgatctgcac cgccctgatc
     1021 gcatcagccg gtttcaacgg ccgcatcgac accaccagcg gaccgctgaa gatgcccgcg
     1081 gatacgcagg gaatgttggt ccggtacatc ggcacgttgc agccgcgggc gatgtgacgg
     1141 acctcgatgc catcctcgat cagcgcgtcc tcgaaggaaa acgagcagcc gatcgcgaaa
     1201 ctgaccagat cgtcccgcca tacgtcccgc acgtcggttg gttcggagat caattttccg
     1261 tcgctccaga cccggtagcg cggcaggtcg gtgcggatat cgagctccgc gcccagagtg
     1321 ggaaagtgcg gatcgcccgc ttcggacacc gccagcaccg ggcatggctt cgggttggcc
     1381 tgggcaaacc gcagaaaatc gtgcgccagc gctttcggca gaatcaccag gttcgcctgc
     1441 acgttccccg gcgccaatcc ggatgtcggg ccggtgaacg ccccggttcg gatacgaagc
     1501 cgctcccgct cgccagcgtt gccgcgcaca tcggtgatag cgttcaaatt ggcctccgtc
     1561 gtcagggcaa cataatggcc ggcgcattgc cagactgcac cgccggcccg aacatgccgg
     1621 tcgcgaacag gctagaaccc gtgttatcgt ggtgcaacag gagggattcc caatgagctc
     1681 acccgcgttc caactcggca agatcggcca cgttgcgttg tacgtcgccg acgtcaaacg
     1741 ttccacgcag ttctacaccg acgtgctggg gttccacgtc tcggatgcct atgaggacga
     1801 catgatgccc ggcggtgccg ccttcctgcg ttgcaacccc gaccaccacg gcatcgcgct
     1861 gttcaaggcg acggagcaaa atccggccgg cgccgggttg catcatatcg ctttcgaggt
     1921 cccgacgctc gacgacgtgg tccgcgcgcg ggcccatctg cgcaagcacg atgtccagat
     1981 tgacttcgat ggccgccgcc gtgcgggcgt gcagatcgcg gtggagttcc gcgatcccga
     2041 caaccaccgg ctcgaaatct actggggaat cgaccagatc ggccccggcg aacaggcccg
     2101 tccggcgcat gagtggaaag gcgccctcag tttggaagcc gccatcgccg atccggtcgt
     2161 cgggcaggac accagcctga aggaccccag ccttctggaa acctgaacgg gacgcctgaa
     2221 ccgccggcga tggttgatca ttgcaggcga tggttgatga tcgcaggcga cgcctggtga
     2281 tcgtggtcgc aggttgatga tcgcaggcaa cggttgatga tcaccggcgg cgcctcataa
     2341 tcgtgtgcgc aggctgatga tcgcaggcga cgcttggcga tcgtggtcgc aggttgatca
     2401 tagtgtcatg gccgtgcttg gcgcggccac ccacgacttt gttgaaccgc ttgaacgccg
     2461 cggctaaccg gcacaggggc cgccatgacg gaggcggccg agcggcgcta gatgtccgca
     2521 gcgtcttcgc attgtcataa agcccgaaca gcgcccagcc gcgacatgtg ccggccacgc
     2581 acagcgagca gcgcgccctg tcgccggcct gacaggcatg gcgaccgccg acctgtttcc
     2641 caccgcttga at
//
LOCUS       DASWQN010001056         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2214312, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001056 DASWQN010000000
VERSION     DASWQN010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214312"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VGC82_18095"
     CDS             complement(<1..166)
                     /locus_tag="VGC82_18095"
                     /inference="COORDINATES: protein motif:HMM:NF044313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425236.1"
                     /translation="MSRQPPGKRADYRWFHAITTRWMDNDVFQHVNNMVYFSWFDTTV
                     TYYEMTEQVVG"
     gene            278..655
                     /locus_tag="VGC82_18100"
     CDS             278..655
                     /locus_tag="VGC82_18100"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEY0425237.1"
                     /translation="MPNCFKTWCVRIRLPILLAAMLFPVLAQAHAILEESVPPQGNKV
                     KAGKLELRLRYNSRIDRGRSRLTLIRPDKGRDTIAIDPGTAPDMIVAHLDLPPGAYVV
                     RWQVLAVDGHITRGDLPITVTAP"
     gene            659..>2069
                     /locus_tag="VGC82_18105"
     CDS             659..>2069
                     /locus_tag="VGC82_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY0425238.1"
                     /translation="MDLLVDVFGYLSIIVHGLTILSQSMALGGVLFLVFLVRPLDSGG
                     LGRQAPSWGIARGTALFAGYAAIALLVCEAATVALQAAVLMDTVELSLRQVLTAEFAV
                     AGLIKTAAAAAIAGLLLGSKERVPTLPLLALSAIELAAATLTTHAAARLDNRGILLVV
                     EGLHQFGAAIWIGGIPCFLLALVRVRDGAAFRLVGARFSRMSMVGVASILVSGITMSL
                     LYIGDWQGLYGTAFGVMVGAKIGMFLMLLALGGMNFLLVERLRVNPATSVNRLRRFAE
                     VEFGIGIAIFFAAASLTSVPPAVDLTQDRVSWQEIRERNTPQWPRLSSPDHDVLALPA
                     LQAKLDQEAATAKAAPQAAFTPGGGELPPRNADDIAWSEYNHHWSGLFVASIGLLALL
                     NRAGVRAARHWPLIFLGLAVFLFFRSDPETWPMGDIGFGESFRDVEVLQHRFYVLLLV
                     AFAFFEWRVRATNWTNRYAA"
BASE COUNT          315 a          640 c          700 g          414 t
ORIGIN      
        1 ggccgaccac ctgctcggtc atttcgtagt aggtgacggt ggtgtcgaac caggaaaaat
       61 agaccatgtt gttcacgtgc tggaacacgt cattgtccat ccaccgcgtg gtgatggcat
      121 ggaaccagcg gtagtcggcg cgttttccgg gtggttgtcg gctcattgcg tcctgcctgg
      181 atgatctggg tcccagttac caaactgccc tcgcattgga cagcggcgcc gccgggcgct
      241 acacggcgcg ccgcctcgct gcctcggaag gatcgccgtg cccaactgtt tcaagacctg
      301 gtgcgtccgg atcaggctgc caatcctgct ggcggcaatg ctgttcccgg ttctggcgca
      361 ggcgcatgcg atcctggagg agagcgtccc gccgcaaggc aacaaggtga aagccggcaa
      421 gctggaactg cggctgcgat acaacagtcg gatcgaccga ggacgatctc gcctgacttt
      481 gatcaggccg gataagggcc gcgacacgat cgcgatcgac ccgggaacgg cacccgacat
      541 gatcgtcgcg cacctggatt tgccgccggg cgcgtatgtg gtccgctggc aggtgctggc
      601 ggtggacgga cacatcaccc gcggcgacct cccgatcacc gtaacggcgc cctgagcgat
      661 ggacctgctc gtcgatgtgt tcggatacct gtcgatcatc gtgcatgggc tgacgatcct
      721 gtcgcagtcc atggcattgg gcggcgtgct gtttctggtg tttctcgtgc gcccgctgga
      781 tagcggtggg ctgggcaggc aggcgccaag ttgggggatc gcgaggggaa cggccttgtt
      841 tgccggttac gcagcgatcg ccctgttggt gtgcgaggcc gccacggtcg cgctccaggc
      901 cgcggttctg atggacaccg tcgagctttc gttgcggcag gtgctgaccg cggagttcgc
      961 cgttgccggc ctgatcaaaa ccgccgccgc cgccgccatc gccggcttgc tgctcggttc
     1021 gaaagagcgc gtgccgacgc tgccgttgct cgcgctgtcc gcgatcgagc ttgccgccgc
     1081 gacgctgacc acgcacgcgg ccgctcggct ggacaatcgc ggcatcctgc tggtcgtgga
     1141 aggcttgcat cagtttgggg cggcaatctg gatcggcggc attccgtgct tcctgctcgc
     1201 cctcgttcgg gtgcgcgatg gagccgcatt ccggctggtc ggcgcgcgct tctctcgcat
     1261 gtcgatggtt ggggtcgcca gcatcctggt cagtggcatc accatgagcc tgctgtacat
     1321 cggcgactgg caaggccttt atggcaccgc gttcggcgtc atggtgggtg ccaagatcgg
     1381 catgttcctg atgctgctgg cgctgggcgg catgaacttc ctgctggtcg agcgcttgcg
     1441 cgttaaccca gcaacctcgg tcaaccgatt gcggcgcttc gcggaagtgg agttcggcat
     1501 cggcatcgcg atcttcttcg cggccgcgtc gctgacctcg gtcccaccgg ccgtggacct
     1561 tacgcaggac cgggtaagct ggcaggaaat cagggagcgc aatacgccgc aatggccgcg
     1621 cctgagcagc ccggaccacg atgtcctggc gctgcctgcg ttgcaggcga agctggatca
     1681 agaggcggca accgccaagg ccgcacctca ggcagcgttc accccgggag gtggcgagtt
     1741 gccgccgcgc aacgccgacg acatcgcctg gtcggaatac aaccaccatt ggtccggcct
     1801 gttcgtcgct tcgatcgggc tactggcttt gctgaaccgc gccggggtgc gcgctgcccg
     1861 gcactggccg ctgatattcc tgggtctggc ggtgttcctg ttcttccgct ccgatcccga
     1921 aacgtggccg atgggcgaca taggattcgg ggaaagcttt cgcgacgtgg aggtcttgca
     1981 gcaccgcttc tacgtgctgt tgctggtggc attcgcgttt ttcgagtggc gggtgagggc
     2041 aacgaactgg acaaaccggt acgcggcct
//
LOCUS       DASWQN010001057         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2610811, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001057 DASWQN010000000
VERSION     DASWQN010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2610811"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /gene="cysD"
                     /locus_tag="VGC82_18110"
     CDS             <1..498
                     /gene="cysD"
                     /locus_tag="VGC82_18110"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY0425239.1"
                     /translation="DEERSRAKERIFSIRAPGHAWDPKRQRPELWHLYNGQLAPGETM
                     RVFPLSNWTELDVWTYIAAEGIDVVPLYFAAMRPTVKRGGQLIVVDDKRMRFEPGEKP
                     VDRMVRFRSLGCYPLSAAVQSEATDMQALLAEMRVTRDSERAGRLIDQEQDSSMERKK
                     REGYF"
     gene            498..2336
                     /gene="cysC"
                     /locus_tag="VGC82_18115"
     CDS             498..2336
                     /gene="cysC"
                     /locus_tag="VGC82_18115"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY0425240.1"
                     /translation="MPDSVLRVLTCGSVDDGKSTLIGRLLFECGTIPDDVMAALERDS
                     KRFGTVDGATDYALLVDGLEAEREQGVTIDVAYRFFETAARRFILADTPGHQQYTRNM
                     VTGASTADLAILLIDARKGILPQTRRHAAIASLLGIRQVVLAINKMDLIGFDEHVFTS
                     IRHDFRALLGTLEFDRITPIPICARDGDNVTSASTRMPWYNGPSLLRTLETADPAVTQ
                     AGPFRMPVQYVSRPDHTFRGYAGTIATGQVRPGDRVVNAGAHTEARVERIVTMNGDLA
                     EAGAGDPVTLVLDREIDISRGDVLTADPAPAAADGLDSWVVWMDETPLFRGRAYLLML
                     GTRSVVATITDISTRLDMETLEEVSVKELALNEIGRVSISLTHPVVCEPYETSRELGG
                     FILIDRLTRSTVGAGMVTATRTRNADIVWHRLDVDKARRSRLMAQKPAVLWFTGLSGA
                     GKSTIANQVEKRLHALGRHTMILDGDNVRHGLNRDLGFSEADRVENIRRIAEVARLFV
                     EAGLIVIVSFISPYRAERMLARDRVEEGEFLEIFVDTPIEECRRRDPKGLYLKADSGQ
                     IRNFTGVDAPYEEPLEAEIRLRTLDASPEALADEVIAALRTRRIIA"
     gene            2392..2934
                     /locus_tag="VGC82_18120"
     CDS             2392..2934
                     /locus_tag="VGC82_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEY0425241.1"
                     /translation="MACRRCEAVLPLGPRPIFQVSDTARILIIGQAPGTKAHNSGVPW
                     NDASGDRLRGWLLMQRDLFYDAARIAIVPTGLCYPGRLPNGGDAPPMPECAPLWLDRF
                     TGSMPHVRLTLLVGSHAQRYRLGKGAMAERVRRFRELGPATIPLPHPSWRTSGWERRN
                     PWFGSDLLPVLRQAVAAALS"
BASE COUNT          516 a          984 c          991 g          518 t
ORIGIN      
        1 gatgaagaac gttcgcgggc caaggagcgg attttctcga tccgcgcgcc gggccacgcg
       61 tgggacccga agcggcagcg tcccgaactc tggcacctgt acaacggcca actggcccca
      121 ggtgagacga tgcgcgtgtt cccgctgtcg aactggaccg agttggatgt gtggacctac
      181 atcgcggcgg aagggatcga cgtggtgccg ctgtattttg ccgccatgcg cccgaccgtg
      241 aagcgcggcg gccagcttat cgtagtggac gacaaacgaa tgcgcttcga gcccggcgaa
      301 aagccggtgg accgaatggt gcggttccgc tcgctgggct gttacccgct atcggccgcc
      361 gtccaatccg aggcaaccga catgcaggcg ctgttggcgg aaatgcgggt gacacgcgat
      421 tccgaacggg cgggccggtt gatcgaccag gaacaggact cctcgatgga acgtaaaaag
      481 cgcgaggggt atttctgatg cccgattcag tcctgcgcgt cctgacctgc ggcagcgtcg
      541 atgacggcaa gtccaccctg atcggacggc tgttgttcga gtgcggcacc attcccgacg
      601 atgtcatggc cgcactggag cgcgattcga agcgcttcgg cacggtcgat ggcgccaccg
      661 actacgcgct gctggtcgat ggcctggaag ccgaacggga gcagggcgtc acgatcgacg
      721 tcgcctaccg cttcttcgaa accgcggctc gccgcttcat cctggccgac acgcccggac
      781 atcagcaata cacccgcaac atggttaccg gcgcctcgac cgccgaccta gccatcctgt
      841 tgatcgatgc ccgcaagggc atcctgccgc agacccgcag gcacgcggcc atcgcctcct
      901 tgcttggcat ccgccaggtc gtgcttgcca tcaacaagat ggatctgatc ggcttcgacg
      961 agcatgtgtt cacctcgatc agacacgact tccgtgcgct gctgggaacg ctggagttcg
     1021 accggatcac cccgatcccg atctgcgccc gtgacggcga caacgtcacc tccgccagca
     1081 cgcgtatgcc gtggtacaac gggccttccc tgcttcggac cctggagaca gccgatccgg
     1141 ccgtgaccca ggctgggccg ttccgcatgc cggtgcagta tgtcagccgg cccgaccaca
     1201 cctttcgtgg ctacgccggc acgatcgcaa ccggccaggt gcggccgggc gaccgggtgg
     1261 tgaacgccgg cgcgcatacc gaagccaggg tcgagcgcat cgtcacgatg aacggcgacc
     1321 tggccgaagc cggggccggc gatcccgtga ccctggtgct ggatcgcgaa atcgacatct
     1381 cgcgcggcga tgtcctgacc gccgaccccg cgcccgctgc cgcggacggg ttggatagct
     1441 gggtcgtctg gatggacgag acgccgctgt tccgcggccg agcctatctg ctgatgttgg
     1501 ggacacgcag cgtcgtggcg acgatcacgg acatctccac ccgcctggat atggagacgc
     1561 tggaggaagt cagcgtcaag gaactggcgt tgaacgaaat cggccgtgtg tccatctcgt
     1621 tgacccaccc cgtggtgtgc gaaccctacg aaaccagccg ggaactgggc gggttcatcc
     1681 tgatcgaccg gctgacccgg agcaccgtgg gtgcgggaat ggtgaccgcg acccgcaccc
     1741 gcaacgccga catcgtttgg catcggctgg acgtggacaa ggcgcgccgg tcgcggctga
     1801 tggctcagaa gccggcggtg ctatggttca ccggcttgtc cggcgccggt aaatcgacca
     1861 tcgccaacca ggtggagaaa cgcctgcacg cgctgggccg ccacacgatg atcctggacg
     1921 gcgacaatgt ccgccatggc ttgaaccggg atctgggctt ttcggaagcc gaccgggtgg
     1981 aaaacatccg gcgcatcgcc gaggtcgcgc gcctgttcgt ggaagccggc ctgatcgtca
     2041 tcgtctcgtt catctcgccg taccgggccg aacgcatgtt ggcacgcgac cgtgtcgagg
     2101 agggcgagtt cctggagata ttcgtggaca cgccgatcga ggaatgtcgg cggcgcgacc
     2161 cgaagggcct gtatctgaag gcagacagcg gccagatacg gaacttcacc ggcgtggacg
     2221 cgccgtatga ggaaccgctg gaggcggaaa tccgcttgcg caccctggac gcatcgccgg
     2281 aggcgctggc cgacgaggtc attgccgcgt tgcgcacccg gaggatcatt gcctgactcc
     2341 tgcataaccg aacctttcgc ggcgcgtctc gcggcggcca gtaccgaagc cttggcctgc
     2401 cgccgctgcg aggcggtgct gccgcttggt ccgcgcccga tcttccaggt ctccgacacc
     2461 gcgcgcatac tgatcatcgg ccaggccccg ggcaccaagg cccacaattc cggcgtgcct
     2521 tggaacgacg catccggcga ccggcttcgt ggctggctgc tgatgcagcg cgacctgttt
     2581 tatgatgcag cgcgcatcgc catcgtgccg accggcctgt gctatcccgg acggctgccg
     2641 aacggcggcg acgccccccc gatgccggag tgcgcgccgt tatggctgga tcgcttcacc
     2701 ggatcgatgc cacatgtgcg gttgacgctt ttggtcggat cccacgcaca gcggtaccgg
     2761 ttggggaaag gcgccatggc cgaacgcgtc aggcggttcc gggaactcgg tccggcgacc
     2821 ataccgctgc cgcacccatc gtggcgcacg tccggatggg agcggcgcaa cccatggttc
     2881 ggttcggacc tgcttccggt gctgcggcag gccgtcgcgg cggcgttgag ctgactccca
     2941 ttcggaccgg gctagaccgt tttccgtttg cctgggctca ctgaaaacgg tctagctcat
     3001 tgttttagc
//
LOCUS       DASWQN010001058         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_894869, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001058 DASWQN010000000
VERSION     DASWQN010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894869"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VGC82_18125"
     CDS             <1..696
                     /locus_tag="VGC82_18125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY0425242.1"
                     /translation="DPFHEPLVTYAYLAGVTKTLGLATGVIILPQRQTALFAKQAADV
                     DLFSDGRLRLGVGVGWNWVEYDGLEMSRHFRRRGKRQEQQIALIRKLWEQPVVDHEDS
                     DHRIDRAGILPRPKRSIPIWLGGFSEAAYDRAARIGDGFMFSGRTQIEAVQIKARITA
                     RLREIGRNADAFGFESIQQYTRGDNQWPGDIAAWRDAGGSHISVVTMGANLTTVDGHI
                     DAIRRWREVYNMV"
     gene            complement(781..>1773)
                     /locus_tag="VGC82_18130"
     CDS             complement(781..>1773)
                     /locus_tag="VGC82_18130"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0425243.1"
                     /translation="TVVLILRRPLEVTMSTIELVDPELRDALEQWPQVPLTAETLSRR
                     RADALALLNAVPKPELPDIATGEIRVESAFGAKPVRVLTYRPVMPDEPLPAIIHIHGG
                     GFVMGAPEMKDVENRLFASDLKCAIYSVDHRLAPEAPHPAPLEDIYSVFVWLHANAGQ
                     LGLDPHRIGIKGESGGGGFAAGAALYARDRQGPKFAFQHLIYPMIDDRSAVRKDLHPY
                     VGEFVWTQANNYFGWRSLLGEEPGAASVSPYAAAARAADVSGLPPTYISVGGLDLFLE
                     ENMTYADRLSRAGVPVEFHVYPRAFHGFYRAPNARVTRQAEHDTREALRRFLHG"
BASE COUNT          338 a          538 c          579 g          318 t
ORIGIN      
        1 gatccattcc acgagccgtt ggtcacgtac gcttaccttg ccggcgtcac caagaccctc
       61 ggtttggcaa cgggcgtgat catcctaccg cagcgtcaga ctgccctgtt cgcgaaacag
      121 gccgcggacg ttgacctgtt ctcagacggt cggcttcggc tgggcgttgg agtgggatgg
      181 aattgggttg aatacgacgg tctcgaaatg tcccgccatt ttcgccgccg cggcaagcgg
      241 caggagcagc agatcgccct gatccgtaag ctgtgggaac agcccgtggt cgaccatgag
      301 gactcggacc accggatcga ccgggccggg atactgccgc gcccgaaacg gagcattccg
      361 atctggctgg gcggtttcag tgaggcggcg tacgaccggg cggccaggat cggggacggc
      421 ttcatgtttt cgggccggac tcagatcgaa gcggtccaaa tcaaggcgag gatcacggcc
      481 aggctgcgcg agatcggacg gaatgccgat gcattcggct tcgaatccat tcagcaatac
      541 acgcgtgggg ataaccaatg gccgggcgat atcgcggcgt ggcgcgacgc tggagggagc
      601 cacatatccg tcgtgacgat gggcgcgaac cttaccacgg tggatggcca catcgatgcc
      661 atcaggcgtt ggcgtgaggt ttacaacatg gtttgacaga atccccgtgg caccgttccc
      721 tgcgacatct tcgccgggac cgaggtgggg ggcgatgagt gccgtcgtgg tgcgcgcggc
      781 ctatccgtgc aggaagcgtc gcagcgcctc gcgcgtgtcg tgctcagcct gtctggtcac
      841 gcgggcattg ggcgcacgat agaagccgtg aaacgcccgc gggtacacat ggaactcgac
      901 tggcacgcca gcccggctca atcgatccgc gtaggtcata ttttcttcga gaaagagatc
      961 cagaccgccg actgagatat acgtcggggg caagcccgac acatccgctg cgcgcgccgc
     1021 cgccgcgtaa ggcgagacac tggccgcgcc cggctcctca ccgagcagcg aacgccagcc
     1081 aaaatagtta ttcgcttgcg tccagacgaa ttcgccgacg taaggatgca gatcctttcg
     1141 caccgccgag cggtcgtcga tcatcggata gatcagatgc tgaaaggcga atttgggccc
     1201 ctggcgatcg cgcgcataca gggctgctcc cgccgcgaaa ccgccgccgc cgctttcacc
     1261 cttgataccg atgcggtggg gatcaagccc gagctgcccg gcattcgcgt gcagccagac
     1321 gaacaccgag tagatgtcct ccaatggcgc cgggtgcggc gcttccggcg cgaggcggtg
     1381 atccacagaa tagatggcgc atttcagatc tgacgcgaac agccggtttt ccacatcctt
     1441 catttccggc gcgcccatca cgaatccacc gccatggatg tgaatgatgg cgggaagagg
     1501 ctcgtcgggc atcacggggc gataggtcag tacccggacc ggtttggcgc caaacgcact
     1561 ctcgacgcgg atttcgccgg tcgcgatgtc gggcagctcc ggttttggaa cggcgttcaa
     1621 cagcgcgagc gcatcggcgc gacgccggct caatgtctcg gcggtcagcg gcacctgtgg
     1681 ccactgctcg agcgcgtcgc gcaattcggg gtcgacaagt tcgattgtgc tcatggttac
     1741 ctccaaggga cgtctgagga tgagcacgac ggt
//
LOCUS       DASWQN010001059         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1423058, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001059 DASWQN010000000
VERSION     DASWQN010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423058"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VGC82_18135"
     CDS             <1..1290
                     /locus_tag="VGC82_18135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0425244.1"
                     /translation="YARPYRVRLGVTRVGVIGGGVVGMICAIELLRDGHAVTVIEPGT
                     PGGEQAASYGNGTLLNPSSVVPMSSPGLWRKVPGYLMDPLGPLTIRWSYLPHMLPWLR
                     RFLMAGSTAARVAATGRALQPLLADAPALHRKLAEEAGVGDLITRQGVLFAFPDRTAF
                     EAEALSWSVRRDNGVKWLELNEDELRQREPTLDRHYKFAVLIEENGQCRDPGAYVAAL
                     ASHAAALGAEFVRTGATGFRIGSGRLRAVTTRDGEVACDKAVIAAGAWSKMLARAAGD
                     RVLLETERGYHVVIADPGIEPRYPMMPSDGKMACVMTPAGLRLAGQVELAGLRAAPDW
                     RRAEVLLKFAREVYPDLPADLSMDRVKVWMGHRPSTPDGLPVLGPASDCADIVYAFGH
                     GHVGLTAAAMTGKIVADVVSERPPPFDLTPYAATRFC"
     gene            complement(1299..1727)
                     /locus_tag="VGC82_18140"
     CDS             complement(1299..1727)
                     /locus_tag="VGC82_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY0425245.1"
                     /translation="MSDSYPGTCFCGAVEIRVTGQPNAMGYCHCRSCRSWSGGPVNAF
                     SLWPPEAVEVVKGAENIATYHKTDMSHRQYCRLCGGHLMTTHPPLKMIDVFAATLPSL
                     AFVPQVHVNYAETVLPMRDGLPKLKDFPSEFGGSGEAVPE"
     gene            complement(1837..>2045)
                     /locus_tag="VGC82_18145"
     CDS             complement(1837..>2045)
                     /locus_tag="VGC82_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425246.1"
                     /translation="AAAHQLSVTCLYTISTAFDRVETEASDREDLALPLSLSIPTSIL
                     QVVPRMVRPSSRQAKLPGQLAHLP"
BASE COUNT          370 a          621 c          686 g          368 t
ORIGIN      
        1 tatgcgcggc catatcgtgt ccgcctcggg gtgacgcggg tcggcgtgat cggcggtggc
       61 gtcgtcggca tgatctgcgc catcgagttg ctgcgcgacg gccatgcggt gacggtcatc
      121 gaacccggca cccccggcgg agagcaggcc gcaagttacg gcaacggcac attgctcaac
      181 ccaagctccg tcgtcccgat gtcgtctccg ggattatgga gaaaggtccc gggatacctg
      241 atggatccgc tgggaccgct gacgatccgc tggtcctact tgccgcacat gctgccgtgg
      301 ttgcggcgct tcctgatggc gggctcgacc gcggccaggg tggcagcgac gggacgggcg
      361 ctgcagccgc tgctggcgga tgctccggct ttgcatcgca aactcgccga ggaagccggg
      421 gtcggcgatc tgatcacccg tcagggcgtg ctgttcgcgt ttcccgaccg aacggcgttc
      481 gaagccgaag cactgtcctg gagcgtgcgg cgagacaacg gcgtcaaatg gctggaactg
      541 aatgaggacg agcttcgcca gcgcgagcca acgctggacc ggcattacaa atttgctgtc
      601 ctgatcgagg agaacggcca atgccgcgac cccggcgcct atgtcgcggc cttggccagt
      661 catgcagccg cacttggcgc ggagtttgtt cgaaccggag cgacaggttt tcgcatcgga
      721 tcggggcggc ttcgggcagt gacaacccgc gacggcgaag ttgcctgcga caaggccgtc
      781 atcgccgccg gcgcctggtc gaaaatgctg gctcgggcgg ccggagatcg ggtgctgctg
      841 gaaaccgaac ggggatacca tgtggtgatc gccgatccgg ggatcgagcc gcggtatccg
      901 atgatgccga gcgacggcaa gatggcatgt gtaatgacgc cggcgggatt gcggctggca
      961 ggacaggtgg agttggcggg cttgcgggcc gcgccagact ggcgccgcgc cgaggtgttg
     1021 ctgaagttcg cgcgtgaggt ctatccggat ctgccggcgg acctttcgat ggaccgggtc
     1081 aaggtatgga tggggcaccg gccgtccacg ccggacggcc tgccggtgct gggtccggca
     1141 tccgactgtg cggatattgt gtacgcgttc gggcacgggc atgtcggtct tacggccgcc
     1201 gccatgacgg gaaagatcgt ggcggacgtc gtttccgagc gtccgccgcc gttcgatctg
     1261 acgccctatg ccgcgacccg gttttgctaa aacacgcgct attccggcac agcctcgccg
     1321 gagccgccga actcggacgg aaaatccttc agcttgggca gcccatcccg catcggcagc
     1381 acggtctcgg catagttgac gtgcacctgc ggcacaaacg ccaaacttgg cagggtggca
     1441 gcgaagacgt cgatcatctt cagcggtggg tgagtcgtca tcagatgacc gccgcagagc
     1501 cggcagtact gccgatggct catgtctgtc ttgtggtagg tcgcgatatt ttccgctccc
     1561 ttgaccacct ccaccgcctc cggcggccat aggctgaaag cattcaccgg accgcccgac
     1621 caggaacgac acgaccggca gtggcaataa cccatggcat tgggctgtcc agtcacgcgt
     1681 atctcgacag caccgcagaa gcatgtccca ggatatgaat cgctcatggt ttgtctcccc
     1741 ttgtttcggc gtcggtgatt cctgagcgaa cgcaggaggc tcagaggacc gaacgtccaa
     1801 tggacgatac cccgatttgg cagaacgcga aaaccttcat ggcaaatggg cgagctgccc
     1861 cggcagcttc gcttgacgcg acgatggccg caccatccgc gggacaacct gcaagatcga
     1921 ggttgggatc gagagagaaa gcggtagggc caagtcctct cgatcgctcg cctccgtttc
     1981 gacccgatcg aacgccgtgg aaatggtgta cagacacgtc accgacaatt gatgtgccgc
     2041 ggcat
//
LOCUS       DASWQN010001060         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2345775, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001060 DASWQN010000000
VERSION     DASWQN010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2345775"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..771)
                     /locus_tag="VGC82_18150"
     CDS             complement(145..771)
                     /locus_tag="VGC82_18150"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0425247.1"
                     /translation="MKAILRTSAICLLFAGSAVALTGERMAEIPLDKMSSAQRAVADA
                     IMSGPRQGIRGPFNAWLRSPVLADRLQKVGEYIRFNTSLDKRVNEMAIIMTAQAWGSQ
                     YEWYAHAPLAIKAGLDPSIVAAIGAGRKPEQMKDDEAVVWEFTTQLRRDHAVDDAIYA
                     KALENFGEQGIMDLIAVNGYYDVVSMTLNVARVAPPAGEELPFKQAGR"
     gene            complement(893..>2405)
                     /locus_tag="VGC82_18155"
     CDS             complement(893..>2405)
                     /locus_tag="VGC82_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY0425248.1"
                     /translation="NMDVLLNVAQGFGVALQPINLLYCFIGVFIGTLVGVLPGIGPIS
                     AMSLLLPVTLSGTPESGIIMMAGIYYGSMYGGSTTSILVNIPGEAASVVTCIDGHQMA
                     KQGRAGPALGISALGSFIAGTFSLVALMLIAPSLANVAIAFGPAEYFSLMVLGLVVLT
                     FLTQGSMAKALLMACIGIVLGLIGLDSITAQPRLTFGRIELIDGIGLVPVVMGLFGIA
                     EVLFNTEQVIKRDIINAKITQLLPSKADWKASAGPVARGTILGFFLGILPGGGAVVAS
                     FASYALEKRLSRTPERFGNGAIEGVAGPESANNAAAGGAFIPLMTLGIPPNVVMALLL
                     GAFVIHGLQPGPLLITQNPGLFWGIVASMYIGNVMLLVLNLPLIGMWVQLLKLPYNIL
                     FPLIILFTIIGVYCSSNNVFDVYVMIAFGVIGYFMRKLGYEPAPLVLAFVLGPMLENN
                     LRKSLILSQGDLFTFVQRPISAACLALAVVLLVGPLLPSLRKKRELVALDEGA"
BASE COUNT          508 a          746 c          716 g          435 t
ORIGIN      
        1 ggatgggtcc atagccccgg cgattgatgc gcgacgcagt tcatggcaaa ggcgttgagc
       61 cggatttctt ttttcatgat tggcaacttt caacgcgctg ccgcgcggca tgactgccgc
      121 gtggcaggtt ataagagata aagactagcg gccggcctgc ttgaacggca attcctcacc
      181 ggccggtggc gcgacgcgtg cgacgttcag cgtcatcgac accacatcgt agtagccgtt
      241 caccgcgatc aaatccatga tgccttgttc gccgaaattt tccagcgctt tggcatagat
      301 cgcatcgtcg acagcatgat cgcgccgcag ctgggtggtg aactcccaga caacagcctc
      361 gtcgtccttc atctgttctg gttttcggcc cgcgccgatg gcggcaacga tcgatgggtc
      421 gaggccggcc ttgatcgcca agggcgcgtg ggcgtaccac tcatattgcg aaccccaggc
      481 ctgcgcggtc atgatgatcg ccatctcgtt gacgcgcttg tcgagcgagg tgttgaagcg
      541 gatatattcg ccgacctttt gcaggcgatc cgccagcacc ggactgcgca gccaggcgtt
      601 gaacggtccg cgaatccctt gcctcggtcc ggacataatg gcgtcggcaa cggcgcgctg
      661 ggcggacgac attttgtcga gcggaatttc cgccattcgc tcgccggtca gcgccaccgc
      721 cgagcctgca aacagcagac aaatggccga cgtgcgcagg atcgctttca tggaaatcct
      781 ccctgatctt cgcccgctct gatttgccgg gctgttaggc cgaaattgtt gcattccggc
      841 tgactttggc aagccaaatt tgtcggcgcg acaatgcctg caggaggccg gcctaagcgc
      901 cttcatccag ggccaccagt tcgcgcttct tgcgcaacga cggcagtagc ggaccgacca
      961 gcagcacgac cgccagcgca agacatgccg ccgagatcgg ccgctgcacg aaggtgaaga
     1021 gatcgccttg cgacaggatc agggatttgc gcaggttgtt ctcgagcatc ggccccagca
     1081 caaatgccag caccagcggc gcgggttcgt agccgagctt gcgcatgaaa tagccgatca
     1141 cgccgaaggc gatcatgacg tagacgtcga acacattgtt gctgctgcaa tagaccccga
     1201 tgatggtgaa caggatgatg agcgggaaaa gaatattgta gggcagtttc aaaagctgca
     1261 cccacatgcc gatcaatggc agattgagca ccagcagcat cacgttgccg atatacatgc
     1321 tggcgacgat gccccagaac aggccgggat tttgcgtgat caatagcggg ccgggctgca
     1381 gtccgtggat aacgaaggcg ccgagcaaca gcgccatcac cacgttcggc gggatgccga
     1441 gcgtcatcag cggaatgaat gcgccgccgg cggcagcgtt attcgcagac tccggacccg
     1501 cgacgccctc gatcgcgccg ttgccgaacc gctccggtgt cctggatagc cgcttctcca
     1561 gtgcgtagga cgcaaatgag gcgaccacgg ctccgccgcc cggcaaaatc cccaagaaga
     1621 atcccaggat cgtgccgcgt gcgaccggtc cggcactggc cttccagtcc gccttgctcg
     1681 gcaacagctg ggtaatcttg gcgttgatga tgtcccgctt gatgacctgt tcggtattga
     1741 acagcacctc ggcgatgccg aacaaaccca tcaccacagg caccagaccg atgccgtcga
     1801 tcagttctat gcggccgaac gttagccggg gctgcgccgt gatactgtcc agcccgatca
     1861 gaccgagcac gattccgatg caggccatca acagcgcctt ggccatcgac ccctgcgtca
     1921 ggaacgtcag aaccaccagc cccagaacca tcaggctgaa atattcggcc ggcccgaacg
     1981 cgatcgcgac gttggcgagt gacggcgcga tcagcatcag cgcgaccagc gaaaatgtgc
     2041 cggcgataaa cgatcccagc gccgagatgc cgagggcggg gccggcccgg ccttgcttcg
     2101 ccatttgatg accgtcgatg caggtcacca ccgaggcggc ctcgccggga atgttgacca
     2161 ggatcgacgt ggtcgatccg ccatacatcg agccgtaata gatgcccgcc atcatgataa
     2221 tgccggactc cggtgttccc gacagtgtga ccggaagcaa cagcgacatc gccgaaatcg
     2281 ggccgattcc cggcagcacg ccgaccaggg tgccgataaa gacgccgata aagcagtaca
     2341 gcaggttgat cggctgcagg gcgaccccaa acccctgcgc gacattgaga agcacgtcca
     2401 tgttt
//
LOCUS       DASWQN010001061         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_761332, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001061 DASWQN010000000
VERSION     DASWQN010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761332"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VGC82_18160"
     CDS             <1..1244
                     /locus_tag="VGC82_18160"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY0425249.1"
                     /translation="PSVQATGGSVPRLAHGIRFAAAAAGFVALMTVALVAAVVALTGF
                     VGTQSYARKAAVTGYLAPMHGVVRVLAPRAGIITAIDVTEGDVVKAGDRLMSVRTQRT
                     AAGGADLDETVRQSLGRQRDLQLHQVSLEQETARSEADRLKERIVRLTQECDAMAMQI
                     AAQRDRNKLAVEQATVSRDLATQGFVSAVEQRRREDARISQAQALSGLNQQLAAKQGE
                     LVELRYALTELPAHSAARVATMRSAAAEIDTRLADAEGQHAYLVVAPAAGRVSAVQAT
                     VGNPADPTHPLLSIVPEGEDLQAVLFVPERAIGFVAPGQSVRLALDAFPFERFGAQEG
                     TITSVAHTLLRPEQLSAVMQLPSEPSYRVTATLRAQSISAYQSRIPLQADMQLHANII
                     FDRRSFVQWLLDPVLSIRGRS"
     gene            1367..>1783
                     /locus_tag="VGC82_18165"
     CDS             1367..>1783
                     /locus_tag="VGC82_18165"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HEY0425250.1"
                     /translation="MRNLIGRRTVPDIRQSEAAECGLACLAMVLGYHGHDVDLGTLRR
                     RHPTSLSGLTMGALMALAGRMDLTARALRLEAEQLGSLALPAVLHWDMDHFVVLTTVK
                     RNGDLVVHDPARGKLRIGSAEASRRFTGVAMELTPTH"
BASE COUNT          317 a          553 c          616 g          297 t
ORIGIN      
        1 cgccctctgt tcaggccaca ggcggttcag ttccacggct tgcgcatggc atccggttcg
       61 ccgctgcggc cgccggtttc gttgcgctga tgacggtggc cctggtcgcg gcagttgtag
      121 cgctgaccgg cttcgtcggt acccagagtt acgcacgaaa agccgcggtc accggatacc
      181 tggccccgat gcacggcgta gtgcgggtgc tggcgccgcg ggccggcatt atcaccgcga
      241 tcgacgtgac cgaaggtgat gtggtcaagg ccggcgacag gctgatgagt gtgcggacgc
      301 agcggacagc agccggcggc gcggatctcg acgagacggt tcgccagtcg ctggggcgtc
      361 agcgcgacct gcaactgcat caggtgtcgc tggaacagga aacggctcga tccgaggcgg
      421 accgcctgaa agaacgcatt gtccggctga cgcaggagtg cgatgcgatg gcgatgcaaa
      481 tcgcagcgca acgggatcgc aacaaactgg cggtggaaca ggccacggtt tcgcgcgatc
      541 tggccactca gggcttcgtc tcggcggttg agcaacgtcg gcgggaagac gcccgaatca
      601 gccaggccca ggcgctgagc ggactgaacc agcaactggc ggccaaacag ggcgaactgg
      661 tggaactgcg ttatgcgctg accgaactgc ccgcgcactc cgccgccagg gtcgccacga
      721 tgcggtctgc cgcggccgag atcgatacgc ggctcgccga tgccgaaggg cagcacgcct
      781 acctggtcgt tgctcccgcg gcaggacggg tgtcggcggt gcaggcgacg gtgggcaacc
      841 ccgccgaccc gacccatcca ctgctgtcga tcgtgccgga gggcgaggac ctgcaggccg
      901 tgctgttcgt tccggagcgg gcgatcggct tcgtcgcccc cggtcagtcg gtgcggctgg
      961 cgttggatgc gttcccgttc gaacggttcg gcgcgcagga gggcacgatc accagcgtcg
     1021 cccacacgct tctgcgcccg gaacaactca gcgccgtgat gcagctaccc agcgagccat
     1081 cgtaccgggt caccgcgaca ctgcgtgcgc aaagcatcag tgcctaccag agccgcatcc
     1141 cgctgcaggc cgatatgcaa ctgcatgcca acatcatttt cgaccgccgc agcttcgtcc
     1201 agtggttgct ggacccggtg ctgagcatcc gcggccggtc ttaacaggct gcggaaaaag
     1261 gcaggttcaa taccactagc agcgtacgta gagtgccgga aaccccaaaa tattgtggat
     1321 catcggggtt tttccgcaac ctgttaagcg caacaggggg atcgccatgc gtaatctgat
     1381 cggccgtcgg acggttcccg atatccgcca aagcgaggcg gctgaatgcg gtctcgcctg
     1441 cttggcgatg gtgctgggct atcacggcca cgatgtcgat ctgggcacgt tgcgcaggcg
     1501 gcatccgaca tcactcagcg gactgacgat gggcgcgctg atggcgctgg ccgggcgcat
     1561 ggacctgacg gcgagggcgc tacggttgga agccgagcag ttggggtcgc tggcgttgcc
     1621 ggcggtgctg cactgggaca tggaccattt cgttgttctg accacagtca agcgcaacgg
     1681 ggacctggtc gtgcatgacc cagcgcgggg caaattgcgg attggatcgg cagaggccag
     1741 ccgccgcttc accggggtgg cgatggaact gacgcccacg cat
//
LOCUS       DASWQN010001062         2740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_235446, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001062 DASWQN010000000
VERSION     DASWQN010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235446"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(356..1399)
                     /locus_tag="VGC82_18170"
     CDS             complement(356..1399)
                     /locus_tag="VGC82_18170"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0425251.1"
                     /translation="MFPITEHVARTGRHTTFYLACGAADATPLIFVHGWPELSISWRH
                     QLPVFAALGFRCIAPDMRGYGRSSVYNQHEDYALEHTVRDMLDLLDALGRDKAVWIGH
                     DWGSPVVWSMGSHHPSRCLGIVNLCVPYLPDGFTPSAVIPLVDRDIYPEAEFPAGQWE
                     YQLFYEENFDKARSVLEASVPDTLKALFRKGNPAGKGQPSRTALVRRDGGWFGGTGRA
                     PVVPRDSDVLTEADLCSYVAALERNGFFGPASWYMNHARNSAYARHATSSGRLAMPVL
                     FLHGAYDYTVETIDSRLAEPMRQYCADLTEVIVQSGHWMAQENPVAVNAALARFLAVK
                     LPAVWRNGSAPTG"
     gene            complement(1633..2175)
                     /gene="ssb"
                     /locus_tag="VGC82_18175"
     CDS             complement(1633..2175)
                     /gene="ssb"
                     /locus_tag="VGC82_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016737891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY0425252.1"
                     /translation="MAGSVNKVILVGNLGKDPEVRSTQDGSKIVNLTLATSETWNDRA
                     SGERKEKTEWHRVVIFNDRTADVAERFLKKGAKIYVEGSLQTRKWTDQQGQERYTTEV
                     VIGRFNGAMTMLDGRAGGGEGASYGSEAASGGGSSYTPRERAPAAAAPARSGGGARGP
                     AGGPSWDAPKGGDLDDEIPF"
BASE COUNT          510 a          862 c          845 g          523 t
ORIGIN      
        1 gtgccggcca tccacgagtt cccgtattga aaccaaggcg tagctggcca agcctacgcg
       61 aaaagcgcgg ttaggtgatc ggcgatccgc ggatgttccg aatcgcccgc gacgacgacc
      121 gcgatgccat actcgcgggc gagcgcgacg aagtcgggtg accatgaatg gttgtggcga
      181 acctcgatct cgatggaggt cagtttgcgg ctggcaatat cagttctcgc cgctgcacca
      241 agcgatcagg atagcacgca ttgcgctatg tttcatcggt cccagccgcc gacgccagcc
      301 tggatacttt gggtcatctg cgacggcata cgggaaccac tttggccggc tgcttttatc
      361 ctgttggcgc cgatccattg cgccacacgg ccggcagctt taccgcaagg aagcgtgcca
      421 gtgcagcgtt gaccgccact ggattctctt gcgccatcca gtggccggat tggacgatga
      481 cctcggtgag atcggcgcaa tattgccgca ttggttcggc gaggcgcgaa tcgatagtct
      541 ccacggtgta gtcgtaggcg ccgtgcagga acaacaccgg catcgcaaga cggccgctgc
      601 tcgtggcgtg cctggcatag gcgctgttgc gggcgtggtt catgtaccag gaggcgggcc
      661 cgaagaaccc attgcgttcc agcgcggcga catagctgca aaggtcggct tcggtcagca
      721 cgtccgaatc ccgcggcacc accggggctc ggccggtccc accgaaccac ccgccgtcgc
      781 gacggacaag cgcggtgcgg gaaggttgcc ctttgcccgc gggattgcct ttgcgaaaca
      841 gcgctttgag cgtatcgggc acactcgctt ccaagacgga gcgggctttg tcgaaattct
      901 cttcatagaa gagctggtat tcccactgac cagccggaaa ctcggcctct ggatagatat
      961 cacggtccac gagtggaatc acggcggacg gggtgaagcc gtcaggcaag tagggaacgc
     1021 agagattgac gatgccgagg cagcggctgg gatgatggct gcccatgctc cagaccaccg
     1081 gactgcccca gtcgtggcca atccagaccg ctttgtcgcg cccaagcgcg tcgagcaggt
     1141 cgagcatgtc gcgaacggtg tgttccaacg cgtagtcttc gtgctggtta taaacgctgg
     1201 agcgtccgta gccgcgcatg tcaggcgcga tgcagcgaaa gccgagggcg gcgaagaccg
     1261 ggagctgatg acgccagctg atcgatagtt ccggccagcc gtggacgaag atcagaggcg
     1321 tcgcatcggc cgctccacag gccaggtaga acgtggtgtg ccgaccggtt cgcgcgacgt
     1381 gttcggtgat tgggaacatg acggccgggt tctcctcatc gcactggtac gctgaccttt
     1441 atagtgcgct ggccggcgtt cggcgaatat gcggcgttgg tggcggtcgc gcggcaagga
     1501 caacgctgcg caggacgacg ctgcgcaaga taccggggcc ctggatgccg tggcctggat
     1561 gccgcgaagg ccatcgccgg cacccaagtt cccgagcctg ggacacggcc gacctgctgg
     1621 cccaggggtg gatcagaacg gaatttcgtc gtccaggtcg ccgccctttg gcgcatccca
     1681 cgagggaccg cctgcgggtc cccgcgcacc accgcccgat cgagctggag cagcagcggc
     1741 tggcgcacgc tcccgagggg tgtacgagga accgccgcca gaggcggctt cagacccgta
     1801 gctggcgcct tcaccgccgc cggcacggcc gtcgagcatg gtcatcgccc cgttgaaccg
     1861 gccaatgacg acttccgtgg tgtaacgctc ctggccttgc tggtcagtcc atttccgcgt
     1921 ttgcagcgac ccttcgacat atatcttggc cccttttttc aggaaccgct ccgccacatc
     1981 cgccgtgcgg tcgttgaaga tcaccacgcg gtgccactcg gtcttttcct tgcgctcacc
     2041 cgaggcgcgg tcgttccacg tttcactggt cgccagcgtc agattgacga tcttgctgcc
     2101 atcctgcgtg gaccggactt caggatcttt ccccaaattc cccaccagga tcaccttgtt
     2161 caccgaacct gccatcgccc caattccttt ccgagactcg aaatcctagc ccaaacatca
     2221 tcctcggacc acaaggttaa aattctcctt acaaactgcc cttccaaagc gcatcgcacc
     2281 ggcgaggtgc cctgggaggt gccctgggag gtgcatgact gagaatcgcg ctattgagtg
     2341 accggtcagg agaacaaatt atgaacttct gaccgggcat gtcaagggcg ccgtgcagca
     2401 atttccttcg gcagccgttc cgcagggtgg tctccgctgg gttgtcaagg accctcgcca
     2461 ggccgatccg gccgaacggc acggcgtccg catgaggctg ttgtgaatcc gatcagtcga
     2521 agcgcggcac atccatgacc gagttcgggc acctcgccgc ccgcgccggc gaacgatcat
     2581 tctttgtagg atgggtccaa tcgttcaata agccgcagac aggccggcca gtcctttcca
     2641 ccgttcagaa aattgccggc atccctgaac tggctgtgag catattcgca agcctccttg
     2701 cttggatcaa gtagttgcct tcacccgctg ccagtgccgc
//
LOCUS       DASWQN010001063         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2214963, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001063 DASWQN010000000
VERSION     DASWQN010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214963"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VGC82_18180"
     CDS             <1..903
                     /locus_tag="VGC82_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425253.1"
                     /translation="NVMKTPHIVLLALDIMDAMGIRYRVMGGPSACCGVLQFGAGDLN
                     TSGRVAYRTIDRLVAAAPRALSWCPTCQIQLTEVAGTGKSDLDMAPFVRFLAERLDTL
                     RPLLRHRVDKRVGLHEHPGNPGVPEAAQAILRAIPGLDFVDLEQPRVGYMCNKLTPLP
                     AFKQDVHRNQLQAAADAGVSTLAGIYHACHRELCSHERDWPFEVVNFLELVGESMGLH
                     RPDLFKRLKIMQDVDAILQDCLPLLATNGLDLEEARDVILRDMLGEQPLPLLEHSTDA
                     AERTRPPGYGSSADRECDFSARLP"
     gene            complement(1032..1334)
                     /locus_tag="VGC82_18185"
     CDS             complement(1032..1334)
                     /locus_tag="VGC82_18185"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEY0425254.1"
                     /translation="MDGDDEITIFEWDEVKNLLNQDKHGVSFEQARHAFADPNRVIFA
                     DIDHSVDEERLFCLGIAGGGVMTVRFTYRLMRIRIFGAGYWRKGKGIYDHERRSLH"
     gene            complement(1413..>2283)
                     /locus_tag="VGC82_18190"
     CDS             complement(1413..>2283)
                     /locus_tag="VGC82_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010501874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HEY0425255.1"
                     /translation="DDPIGRQFIPDPAELVTEAFEHADPIGDDALSPVKGVVHRYPDR
                     ALLKPLLVCPVYCRFCFRREHVGPDGGVLTDNELQAAYAWFAAHPEVTEIILTGGDPL
                     MLSPRRLGGIIAALSAMPHVQTIRIHSRVPVADPERLTAQLAAAMETDRSLWLVVHAN
                     HAREFTPAAAAALRRVRSLGIPLLGQSVLLRGVNDSSVALEALFRAMIAARMKPYYLH
                     QLDRAPGTARFHVPIEEGRALLEALRGRVTGLAWPTYVLDIPGGYGKVPVGPDYRQAD
                     GAVRDIRGAVHRA"
BASE COUNT          449 a          749 c          651 g          434 t
ORIGIN      
        1 aacgtcatga agacgccgca catcgtgtta cttgcgctcg acatcatgga cgcgatgggc
       61 atccgctacc gggtcatggg cggtccgtcc gcctgttgcg gcgtcctgca gtttggtgcc
      121 ggagacctga atacatccgg ccgcgtcgcc taccggacca tcgatcgtct ggtagcggca
      181 gccccacgcg cgttgtcctg gtgcccgacc tgccagatac agttgacgga agtcgcagga
      241 acgggaaaat cggacctgga tatggctccg ttcgtccgct tcctcgcgga acgtctggac
      301 acgctgcgcc cgctactgcg acaccgcgtc gataaacgcg tcggcctgca tgagcatccc
      361 ggcaatcctg gtgtacccga ggccgcacag gcaatcctgc gcgccatccc cggtctcgat
      421 ttcgtcgatc tggagcagcc acgcgttggt tatatgtgca acaagctcac gccacttccg
      481 gcgttcaagc aggatgttca ccgtaatcaa ctgcaagccg ccgccgatgc cggggtttcg
      541 actctggccg gcatctatca tgcatgccat cgggaactgt gcagtcacga acgcgactgg
      601 ccattcgagg tggtgaactt cctggaactg gttggcgaga gcatggggtt gcaccgcccc
      661 gacctgttca agcgcctcaa gatcatgcag gacgtcgatg cgatccttca ggactgcctt
      721 cctttactcg cgacgaacgg tctggacctg gaggaggccc gggacgtgat cctacgcgac
      781 atgctcggcg aacagccgct gccgttgctc gagcattcta ccgatgccgc cgagcgtact
      841 cgtccaccag gctacggatc atccgctgat agggaatgcg atttttccgc gcgacttcct
      901 tgaaaaaatc cacgctctgg cggctcaggg tcagcgtcac cttcactccg tcgtcgggga
      961 gtacaagctg atccggtggc ggcagcgtat cggcgacccg gtggccgatg ctgtccattt
     1021 cgctaaggtc gtcagtgtag cgaacgtcgt tcatggtcat agatcccttt acccttgcgc
     1081 cagtaaccgg cgccgaaaat tcggatgcgc attaaccgat aggtaaaccg gaccgtcatc
     1141 acgcctccgc ctgcgatgcc gaggcaaaag agccgttctt cgtcgacgct gtggtcgatg
     1201 tcggcaaaaa tcacccgatt tggatccgcg aaggcatgtc tggcttgttc gaagctaacg
     1261 ccgtgtttgt cctgattgag gaggttcttt acctcatccc actcgaatat ggttatctcg
     1321 tcatcgccgt ccatatatcg caccatataa atatatggca aggaaaattc ctgccgtcgg
     1381 cgcgataaag cccacaattc cataacaaac tgctaagccc gatgaacggc tcctcggatg
     1441 tcccgcaccg ccccatctgc ttggcggtaa tccggtccga ccggcacctt gccgtatccg
     1501 ccagggatat ccagcacgta agtaggccac gccaatccgg tcactcggcc gcgcaatgct
     1561 tccagcaaag cccgcccctc ctcgatcggg acgtgaaacc gggcggtgcc aggcgcgcgg
     1621 tccaactgat gcaggtaata cggcttcatc cgcgcagcga tcatcgctcg gaacagtgcc
     1681 tctaacgcaa ccgaactgtc gttcacgcct cgcagcagca ccgactgccc cagcaatggg
     1741 atccccagcg accgcacccg acgcagcgcc gcggcggcgg ccggtgtgaa ttcccgagcg
     1801 tggtttgcat gcacgaccag ccacagcgac cggtcggtct ccatcgccgc agccagttgg
     1861 gccgtcagcc gttccgggtc agccaccggc acgcgcgagt ggatacggat cgtctggaca
     1921 tgcggcatcg ccgacagcgc cgcgatgatc ccacccagcc gccgaggcga cagcatcagc
     1981 gggtcgccgc cggtcagaat gatttcggtt acctccgggt gggcggcgaa ccaggcataa
     2041 gccgcctgca gttcgttgtc cgtcagaacc ccaccatcgg gcccgacatg ttcccggcga
     2101 aaacagaacc ggcaatacac cgggcacacc agcagcggct tcaacagcgc ccgatccggg
     2161 tagcgatgca ccacgccctt gaccggagac agcgcatcgt cgccgattgg atcggcgtgt
     2221 tcaaacgcct cggtcaccag ttccgccgga tcgggaatga actgcctgcc gatcggatca
     2281 tcc
//
LOCUS       DASWQN010001064         4872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2215020, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001064 DASWQN010000000
VERSION     DASWQN010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2215020"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VGC82_18195"
     CDS             <1..349
                     /locus_tag="VGC82_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY0425256.1"
                     /translation="EFRGFRYETGVSVAVYAPGRHEELGRGGRYICGDSEPATGLTLY
                     PDAILRVAPARQARPRVFVPFGADRGAASGLRADGYATVAALEAVADDTAEACRLGCS
                     HVLRAGTLLHIED"
     gene            354..1643
                     /locus_tag="VGC82_18200"
     CDS             354..1643
                     /locus_tag="VGC82_18200"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY0425257.1"
                     /translation="MGNVAVIGAQWGDEGKGKVVDWLASRADVVVRFQGGHNAGHTLV
                     VGNETYKLSLLPSGLVRGKLGIIGNGVVVDPDALLSEIARVRTQGLTVTPETLRIAEN
                     AVLILPLHPALDKAREEDRGDRKIGTTGRGIGPAYEDKVARRAIRVADLAEPETLSAK
                     LDELLLHYNTLLQGLGVAPFSKDILLDGLLKLAPQILPFAEPVWERLDELRRGGKRIL
                     FEGAQAVMLDVDHGTYPFVTSSNTVAATAASGSGVGPGAVGFVLGIAKAYTTRVGSGP
                     FPTELRDEIGQMLGDRGREFGVVTGRRRRCGWFDAALVRQAIKVGGVQGLALTKLDVL
                     DGLAELKICTGYRIGGTFFRHLPAAPGAQAAAVPEYESVEGWSGSSRGARSWADLPAQ
                     AVKYVRRIEELVEAPVTLLSTSPERDDTIMVKDPFEG"
     gene            complement(2126..3070)
                     /locus_tag="VGC82_18205"
     CDS             complement(2126..3070)
                     /locus_tag="VGC82_18205"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0425258.1"
                     /translation="MGTAKSSNGTSKPKLKAPPASIDPFPDTPPRDYNIAAVERTLDL
                     LEVLSRLGPSSLAALAESAGCTRTAAFRLLRTLQGRGFTIQDDARGVWRLGARWSALG
                     RAASEQGALAATAMPILGALGKATGENVYLRVRDGMESETVCIYQTDPTIRMYSEVGK
                     RGPLHAGSSRILLAFAPEAVQTQLLAQRLNRYTPATRIDPTWIAADLQRIRTRGFLIT
                     SDEVVAGAVGIAAPVRDATGTVVAVLYISAPSMRMRPPRPRNLLPQVIDAAAKLSQAL
                     GASVKPAQPGLVRDASAPAGWPMNGGMLTQSRPHTIFR"
     gene            3375..3932
                     /locus_tag="VGC82_18210"
     CDS             3375..3932
                     /locus_tag="VGC82_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0425259.1"
                     /translation="MAKLLSRPPERLHHHAYVVRDQEKNRRFFEDVLGIPLTATWCER
                     HLSPWVNREVAFCHTFYSLGDGGALAFFSFADEEVYQKCQAASPPEIGSFDHIAFKVD
                     DGTYDELVSRVKAANEPVRETDHGYCRSMYCTSPDGLIVEFTVDAPDVAEINALRRAD
                     AHSELARWMAGDHRTNNELRTHQAA"
     gene            4092..4175
                     /locus_tag="VGC82_18215"
     tRNA            4092..4175
                     /locus_tag="VGC82_18215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4125..4127,aa:Leu,seq:tag)
     gene            4243..>4872
                     /locus_tag="VGC82_18220"
     CDS             4243..>4872
                     /locus_tag="VGC82_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY0425260.1"
                     /translation="MQVTETLSDGLKRNFTVVLPMADLESRRSARLTTLGKTLRLPGF
                     RPGKVPMPIVKQRYGTAVSAEVLEESVSEATQKVLNERGLRPAQQPKVDLVTENPTAM
                     ASDLEFKVELELLPDIVLPDFSTIELTRLKAEVAPEAVDAALTQLAKYNRTLEPIPPE
                     TIESRGSGAMAGEVLTVDFEGKIDGVPFEGGTGTDSPVEIGAGDFIPGFA"
BASE COUNT          833 a         1519 c         1623 g          897 t
ORIGIN      
        1 ggaattccgc gggttccgct atgagacagg tgtgtccgta gcggtttacg cgcccggccg
       61 gcacgaggag ttggggcgcg gcggccgcta catctgcggc gacagcgaac cggcgaccgg
      121 cttgacgctg tatccggatg cgatcctgcg ggtggcgcct gcgcgccagg cacgtccgcg
      181 cgtgttcgtg ccatttggcg ccgatcgcgg tgccgcgtct ggtctgcgcg ccgatggcta
      241 cgccacggtt gccgccctgg aagcggttgc cgacgatacg gccgaggcat gccgcctcgg
      301 ttgctcccat gtcctgcggg ccggcacgct tcttcacatc gaggattgaa caaatgggca
      361 atgtcgccgt gatcggtgcc cagtggggcg acgaaggcaa gggaaaggtc gttgactggc
      421 tcgccagccg agccgacgtg gtggtccgtt ttcagggtgg ccataatgcc gggcacacgc
      481 tggtcgtcgg caacgagacc tataagttat cgctgctgcc atcggggctg gtgcgcggca
      541 agctcggcat catcggaaat ggcgtcgtgg tcgatccgga tgcgctgctg tccgagatcg
      601 ctcgggtgcg gacccagggc ctgacggtca cgccggaaac gttgcgcatc gccgagaacg
      661 ccgtgctgat cctgccgctg cacccggcgc tggacaaggc gcgcgaggaa gaccggggcg
      721 accggaaaat cggcaccacc ggccggggta tcggtccagc ttacgaggac aaggtcgccc
      781 gtcgcgccat ccgtgtcgcc gacctcgcgg agcccgagac actgtcagcc aagctcgacg
      841 aactgttgct gcattataac acgttgctgc aaggcctcgg tgtcgcgcca ttcagcaagg
      901 acatcctgct ggatgggttg ctgaagctgg cgccgcagat cctgccgttc gccgaaccgg
      961 tgtgggagcg gctggacgaa ctccggcgcg gcggcaagcg gatcctgttc gaaggcgccc
     1021 aggccgtgat gctggacgtc gatcacggaa cctatccgtt cgtcacgtcc tctaacacgg
     1081 tcgcggcgac agcagcgtcc ggcagcggcg tgggccccgg agcggttggc ttcgtgctgg
     1141 ggatcgccaa ggcctatacc acgcgggtcg gttcaggccc gtttcccacg gaactgcgtg
     1201 acgagatcgg ccagatgctc ggcgaccgtg ggcgcgaatt tggcgtcgtt accggccgcc
     1261 gccgccgttg cggctggttc gatgcggcgc tggtgaggca agccatcaag gtcgggggcg
     1321 tgcagggcct cgcgttgacc aagctggacg tcctggacgg gctggcggaa ctgaagatct
     1381 gcaccggcta tcggatcggc ggcactttct tccgccacct gccggcggcg ccgggtgcgc
     1441 aggccgccgc tgtgccggag tatgaatctg ttgaagggtg gtccgggtca tcgcggggcg
     1501 cgcgatcctg ggccgacctg ccggctcagg ccgtgaaata tgtccggcgg atcgaggagt
     1561 tggtggaagc tccggtcact ttgctgtcca cgagcccgga gcgggacgac accatcatgg
     1621 tgaaggaccc gttcgaaggg tagatcgtcc cgagaacagc gactgtcgcc acggcagtcg
     1681 ttgccggctc tgtaccgaac atcccgactt tccgcgagca gacggctttc cgcgagcaga
     1741 caaagtcgtg gtggccgcgc caagcaccgt catgacactg ttatcgagca gccgatcgct
     1801 cgatcccggg caccgccaga acccattggc gctcgtgacg cctacgcccc catgatcgga
     1861 ggccccagct gaagcggtta tctcgccgcg gatgctcaga tcattttcgg cctgactgcg
     1921 caggccgcag atgatctacc ttcttgtttt gtcgcgtgat tcacacgctt tggacgctcc
     1981 gccagcgtaa tcactctctg gtgtcccgaa tctgaattcc gacatatccg gcggacttca
     2041 gtctcgcgcc actctctttg ttttcagtgg cctgctgaac cccctgcgtc cggcgcatga
     2101 tgcgacgaac ttcgggggca ggacactagc ggaagatcgt atgcgggcgg gactgcgtca
     2161 gcattccacc gttcattggc caacccgccg gggccgatgc gtcgcgcacc aaacccggct
     2221 gagccggctt gaccgatgcg cccagcgcct gggacagttt cgccgccgcg tcgatgacct
     2281 gcggcagcag gttgcgcggc cgcggcggac gcattcgcat tgacggcgca cttatataga
     2341 gcaccgccac cacggttccc gtcgcgtcgc ggaccggggc ggcgatgcca acggcaccgg
     2401 cgacgacctc atccgacgtg atcaaaaagc cccgcgtccg gattcgctgc agatcggcag
     2461 cgatccaggt cgggtcgatg cgcgtggcag gcgtgtagcg gttcaggcgc tgagccaaaa
     2521 gctgggtctg caccgcctcg ggcgcgaacg ccagcaggat gcggctgctg ccggcgtgca
     2581 acgggccgcg tttgccgact tccgagtaca tgcgaatggt cggatcggtc tgatagatac
     2641 agacggtttc gctttccatg ccatcgcgga cgcgcagata gacgttctcg ccggtggcct
     2701 tgccaagcgc gcccaggatc ggcattgccg ttgctgcaag cgctccttgt tccgaggccg
     2761 cccggcccag agcgctccat cgcgcaccca ggcgccaaac gccgcgggcg tcgtcctgga
     2821 tggtgaaccc gcgcccttgc aacgtccgca gcaggcggaa cgcggcggtt cgcgtgcatc
     2881 cggcactctc ggccagtgcg gcgagcgatg agggacctag gcgggacagc acctccagca
     2941 ggtcgagcgt gcgttcgacg gcggcgatat tgtagtctcg gggtggcgtg tcagggaacg
     3001 ggtcgatcga tgcggggggc gccttgagct ttggcttgga agtcccattg ctgcttttgg
     3061 ctgtccccat cgtctcttcc cttttgctcg agatgccgag tggtcttgtc gcacgaagcc
     3121 gtgcgtcgaa cccgtttcgc tcgcatcgat tacttgccgg tagaattaca ttccttaatg
     3181 gtccccgcaa aaggcctctc tgcatgggtg ctatgcacat gggcgtttga tgacgaaata
     3241 accagttgca tgccgccctg gcaccagact gggttacagc ccgggcaccg gaccgcggca
     3301 ccagactagg acgccagatt tgcgtcaccc cgcgcgctgc ggcaaaatgt ccgcgaagaa
     3361 aaaaggggag agcgatggcc aaactcttgt cacgtccacc ggagcgcctg catcatcacg
     3421 cctatgtcgt ccgcgaccag gaaaagaacc gccgcttttt cgaggacgtg ctcggtatcc
     3481 ccctcaccgc cacatggtgc gagcgccacc tcagtccctg ggtcaaccgt gaagtcgcct
     3541 tctgtcacac tttctatagc ctgggcgacg gcggtgcctt ggcctttttc tccttcgccg
     3601 acgaggaggt gtatcaaaag tgccaggcgg ccagcccgcc ggagatcggc agtttcgacc
     3661 acatcgcgtt caaggtcgat gacgggacct atgacgagct ggtcagccgg gtgaaggcgg
     3721 cgaatgagcc ggtgcgcgaa accgaccacg gttattgccg ctcgatgtac tgtacctctc
     3781 cggacggcct gatcgtcgaa ttcactgtcg atgcgcccga tgtcgccgag atcaacgcgc
     3841 tgcgccgggc tgacgctcat tccgaacttg cccgctggat ggccggcgat caccgcacca
     3901 acaacgagct gcggacgcac caggcggcat agcggcgatt cgcctgtcga agtggcggcg
     3961 gcggttatcg gcatcagcct gctcgccgct tgttgcttgt cgtcatgcct agcctggggt
     4021 aaagcgccgc gttcggtccg tgccggttgg gttccggagc agaagcgttc cggagcgagg
     4081 gccggcggtg tgcgggcgtg gtgaaatggt agacacgcca gatttaggtt ctggtggcga
     4141 aagccgtggg ggttcaagtc ccttcgcccg caccagaccc ccctcgcgct gcgctggacg
     4201 gacggctgag ctaaacggat tttgttagga ttggctgtga caatgcaggt taccgagacg
     4261 ctttccgacg gattgaagcg caatttcacc gtggtcctgc cgatggcaga cctggaatcg
     4321 cgccgctccg cgcggctgac gacattggga aagacgctgc ggttgccggg gttccgcccg
     4381 ggcaaagtgc ccatgccgat cgtcaaacag cgctatggaa ccgcggtgtc ggccgaagtg
     4441 ctggaggagt cggtttccga agcgacacag aaggtcctga acgaacgcgg tttgcgtccg
     4501 gcgcagcagc ccaaggtcga tctggtgacc gaaaacccga ccgcgatggc cagcgacctg
     4561 gagttcaagg tcgaactgga attgctgccc gacatcgtcc tgcccgactt cagcacgatt
     4621 gagttgacgc gcctgaaggc cgaagtggcc ccggaagcgg tcgatgcggc gctgacccaa
     4681 ctcgccaagt acaaccgcac gctggaaccg atcccaccgg aaacgatcga aagccgtggg
     4741 tccggcgcga tggccggcga ggttctgacc gttgatttcg agggcaagat cgacggcgtt
     4801 ccgttcgagg gcgggaccgg caccgattcg ccggtggaaa ttggcgctgg ggattttatc
     4861 ccgggttttg cc
//
LOCUS       DASWQN010001065         4929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1423583, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001065 DASWQN010000000
VERSION     DASWQN010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423583"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VGC82_18225"
     CDS             complement(<1..278)
                     /locus_tag="VGC82_18225"
                     /inference="COORDINATES: protein motif:HMM:NF019187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY0425261.1"
                     /translation="MLYFTRWKALAIILTALVVCLCAVPNFFPEATVKGWPAWAQRRL
                     VLGLDLQGGSYLLLEVDSNYVKKEKLDQVRDDVRRTLRDAKIGYTGLAT"
     gene            complement(321..686)
                     /gene="yajC"
                     /locus_tag="VGC82_18230"
     CDS             complement(321..686)
                     /gene="yajC"
                     /locus_tag="VGC82_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY0425262.1"
                     /translation="MLITPAYAQAAGGGDTNSMLMSLLPFALIFVIMYFLILRPQQKK
                     VKDHAELVKNIRRGDTVVTSGGLVGKVTKVVDDDQIEFEISDGVRVRQMRQMISGVRA
                     KGEPAKEKSEASKDETSAS"
     gene            826..1833
                     /locus_tag="VGC82_18235"
     CDS             826..1833
                     /locus_tag="VGC82_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0425263.1"
                     /translation="MQPAERPDMAAWSGTPKMSKKSRKTPPRATRKSAAAATKRPAAA
                     SEHDTAERIAQALEVIAAHLSATSAIPAAADAFGSADAFVWHPDGRLAPVPRVSRVDL
                     GLLRGIDRMRDILIENTERFADGLPANNALLWGARGMGKSSLVKATHASINVDRKPAD
                     RLKLIEIHREDIESLPALMDQLRASEFRFIVFCDDLSFDGNDASYKSLKAVLEGGIEG
                     RPDNVILYATSNRRHLLARDMIENERSTAINPGEAVEEKVSLSDRFGLWLGFHRCSQD
                     EYLAMVKGYCGHFGIRIAADELERDALEWSTTRGSRSGRVAWQFTQELAGRLGVRLAG
                     Q"
     gene            complement(2047..3372)
                     /locus_tag="VGC82_18240"
     CDS             complement(2047..3372)
                     /locus_tag="VGC82_18240"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HEY0425264.1"
                     /translation="MSRAVELLCSRRIPHVAVLALMSVGFAGCSADMSTRFSQNSYSS
                     PFASQRETTGAVPAATVERRELPQYSRPPLRNAAAAPPSVSAPQTYPTAGGVTGGGRG
                     LASYAPPTRPIETTGALAPRSVAAARSPNQAGTTIIVGTSDTLEGLARRYHVSPAAIL
                     QANGYRGPRALSPGQQLIIPRQTANAPAPALAAPATRPIAAVAATSVHVVNRGDTLLS
                     VARRNHVPVSELARANGLDPQAKLRLGAKLTVPAARTAAASPVLQPEPVAVAPPAPAT
                     RMAAAAIGPQQSVRLAQATTRVEEPAVETPAKSAEATGALPTFRWPVRGKVITSYGAK
                     TNGKANDGINLAVPEGTPVKAAEDGVVAYSGNELKGYGNLVLVRHTNGYVTAYAHASE
                     LMVKRGDTIKRGQIIAKSGQSGEVGSPQLHFEIRKGSSPVDPLQFLNGA"
     gene            complement(3593..4243)
                     /locus_tag="VGC82_18245"
     CDS             complement(3593..4243)
                     /locus_tag="VGC82_18245"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY0425265.1"
                     /translation="MAPNHDPSEKMKFQLILRRRGISDQAVLRAMEEIPRELFVEPAD
                     RDDAYRDSALGIACGQTISQPFVVGYMTEQLQLRKHHRVLEIGTGSGYQAAILSRLCA
                     DVLTIERYRTLADNARARLRTLGYGNVEVRLGDGFDIPDTAGTFDRIIVTAAMEQIPD
                     ALMERLEPAGILLAPVGSHHGTQTLVRINRTETGFDRKELVDVRFVPALRGIAREL"
     gene            4494..4871
                     /locus_tag="VGC82_18250"
     CDS             4494..4871
                     /locus_tag="VGC82_18250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0425266.1"
                     /translation="MTAAQPPGGSVFLVEDEVMIRMMVADMLEELGYSVAAEAGEIKE
                     AVRMAQSAEFDLAILDVNVNGKVISPVADLIKARNLPFIFATGYGSSGLPEEYRDRPA
                     LQKPFQLETLARMIDTALKSTAA"
BASE COUNT          893 a         1556 c         1600 g          880 t
ORIGIN      
        1 gtcgccagcc cggtatagcc gatcttggcg tcgcgcaacg tgcggcgaac atcgtcgcga
       61 acctggtcga gtttctcttt cttgacgtag ttggaatcca cctcgagcag caggtaggag
      121 ccgccctgca ggtcgaggcc gagcaccagc cgccgctgcg cccaggccgg ccagcccttc
      181 acggtagctt cggggaagaa gttcggaacg gcgcagaggc acaccaccag cgccgtcaga
      241 atgatcgcca gcgccttcca acgcgtgaaa tacaacatcg agttgacccg tcaaatccag
      301 gagaatgcgg cgtgcaaaag ctagctcgcg gacgtttcgt ccttgctggc ttcgctcttt
      361 tccttcgccg gttcgccctt ggcgcgcacg cccgagatca tctgccgcat ttgccgcacc
      421 cggaccccgt cggagatttc gaattcgatc tggtcgtcat cgaccacctt ggtgaccttg
      481 ccgaccaggc cgccggacgt gacgacggta tcgccgcggc ggatattctt cacgagttcg
      541 gcgtggtcct tcaccttctt ctgttgcggc cgcagaatca ggaagtacat gatcacgaag
      601 atcagcgcga acggcagaag cgacatcaac atgctgttgg tatcgccacc gcccgcagcc
      661 tgggcatacg caggtgtaat cagcattcgg acaatcctcg tgaaacggga atgggccggc
      721 gacgcatccg catcacgccg gttcggcaaa ttcgcgcgga ctatagcggc cgcggtccca
      781 attgcaacgc tgcctgagcc gtcatttgcc cttgattaga gcggcttgca gcctgccgaa
      841 aggcccgata tggctgcgtg gtcaggaact ccgaagatgt ccaaaaagtc ccgaaaaacg
      901 ccacctcgcg ccacgcggaa atcagcggcc gccgcgacga aacgtccggc ggcggcttcc
      961 gaacatgaca cggcggaaag gattgcgcag gcgcttgaag tcatcgccgc gcatctgtct
     1021 gccacttccg caataccggc ggcagccgac gcattcggca gcgccgacgc ctttgtctgg
     1081 catcccgacg ggcggctcgc gccggtgccg cgggtcagcc gcgtcgatct cggcctgctc
     1141 aggggcatcg accggatgcg cgacatcctg atcgagaata ccgaacgttt cgccgacggc
     1201 ctgcccgcca acaatgcgct gttgtggggc gcgcgcggca tgggcaagtc gtcgctggtc
     1261 aaggcgacgc atgccagcat caatgtcgac cgcaagccgg ccgaccggct gaagctgatt
     1321 gaaattcacc gcgaggatat cgagagcctc ccggcgctga tggaccagct gcgcgcttcc
     1381 gagtttcgtt tcatcgtgtt ttgcgacgac ctctcgttcg acggcaacga cgcctcctac
     1441 aaatcgctga aagcggtgct ggagggtggc atcgaaggcc gccccgacaa tgtgatcctg
     1501 tacgccacct ccaaccgccg gcacctgttg gcgcgcgaca tgatcgagaa cgaacgctcc
     1561 accgcgatca accccggcga ggcggtcgag gaaaaagtct cgctctccga ccggttcgga
     1621 ctgtggctcg gcttccaccg ctgcagccag gacgagtatc tggcgatggt gaagggctat
     1681 tgcggccatt tcggcatcag gatcgccgcg gacgaacttg agcgcgatgc actggaatgg
     1741 tcgaccacgc gcggctcgcg ttcggggcgc gtcgcctggc agttcacgca ggaactggct
     1801 ggaaggcttg gggtcaggct ggcggggcag tagcacgcgg ttttaccgaa aggtcacctg
     1861 ccagccacag ctcatcattc ctcgccaccg ggtctcacct tcgaggagcc caatgacagg
     1921 ctccagcgcg gattccagta cgccgcggcg ccgcggctcg accatacgtc tgtggaatag
     1981 cgggtccgcc cggtcagccc ggcgacggca ggggtcgaat cgtcgtggcg cctggtgaag
     2041 ggcgccttac gccccgttca ggaactgaag cgggtcaacc ggtgaagatc ctttacggat
     2101 ctcgaaatgg agttgcggcg accccacctc gcccgactga cccgatttgg caatgatctg
     2161 gccgcgcttg atcgtatcgc cgcgcttcac catcagttca ctcgcatggg catacgcggt
     2221 gacgtaaccg ttggtgtgcc gaaccaggac caagttgccg taacccttga gttcatttcc
     2281 ggaataggcc acgacgccgt cttccgcggc cttcacgggc gtgccttccg gcaccgccag
     2341 gttgataccg tcattggcct tgccgttggt cttggcgcca tagctcgtga tcaccttgcc
     2401 gcgcacgggc cagcggaatg tcggcagtgc accggtggcc tcggcggact tggccggcgt
     2461 ttcgaccgcg ggctcttcga ccctggtggt ggcctgggcc agccgaacgc tttgttgagg
     2521 accgatggcc gcggcggcca tccgggtggc cggcgccggc ggtgcgacgg cgacgggctc
     2581 cggctgcaac accggggacg ctgccgcggt cctcgcggct ggcactgtca gcttggcgcc
     2641 gagcctgagc ttggcctgcg gatcaaggcc gttggctctt gccagttccg acaccggcac
     2701 gtggttgcga cgggcgacac tgagcaaggt atcgccacga ttgacgacat ggaccgaggt
     2761 cgccgccacg gcggcaatcg gcctggtggc gggcgccgcg agcgcggggg ccggtgcgtt
     2821 cgcggtctgg cgcgggatga tcagctgttg tcccggcgac agggcgcgag gtcccctgta
     2881 accgttggcc tgcaggatgg cggccggcga cacatgatag cggcgcgcca ggccctcgag
     2941 cgtatcgctg gtgccgacga tgatcgtggt gccagcctgg ttcgggctgc gggcggccgc
     3001 gaccgaacgc ggagccagtg cgcccgtggt ctcgatcgga cgcgtcggcg gagcgtagga
     3061 cgcgagcccg cgcccacctc cggtaacgcc acccgcggtg ggataggtct gcggcgccga
     3121 gaccgagggc ggcgcggctg cggcattgcg caatggcggg cgggaatatt gcggcagttc
     3181 gcgacgctcg accgtggcgg ccggcaccgc gccggtggtt tcgcgctgcg aggcaaaggg
     3241 gctggaatag gagttttgcg agaagcgcgt cgacatgtcg gcgctgcagc cggcaaagcc
     3301 aacggacatc agggccagca ccgcgacgtg cgggatacgg cgcgagcaca gcaactcgac
     3361 ggcacgggac atggttactc actcgtacgc aacacgatac tgttttgagt aaacacctgc
     3421 ccagtaaaca accacttaat cgggccaata agacgctggc ggaacagcca aagcgggaac
     3481 ttcggtttgc tcaccaaggg cgagcggcgc gcgcttgcac gggcctccac gcggccagga
     3541 tgccgacccc ttgacggtcc tcgggttgaa cgattggcgg ggtgatagag cgctagagtt
     3601 cgcgggcgat gccacgcaac gccggcacga agcgcacatc caccagttct ttccgatcga
     3661 agcccgtctc cgtccggttg atgcggacca gggtctgggt gccgtgatgg gatccaaccg
     3721 gggcgagcag gatgccggcg ggctcgagcc gctccatcaa agcgtccgga atttgctcca
     3781 tcgctgcggt gacgatgatg cggtcgaaag tcccggcggt gtcgggaatg tcgaaaccgt
     3841 ctcccagcct cacctcgaca ttgccgtagc cgagcgtcct cagtcgggcc cgggcgttat
     3901 cggccagcgt ccggtaacgt tcgatggtca atacgtccgc gcaaaggcgg gacaggatcg
     3961 ccgcctggta gccggagccg gtgccgatct cgagcacgcg gtggtgcttc cgaagctgaa
     4021 gctgttcggt catgtagccc accacaaacg gctggctgat ggtctgcccg caggcgatgc
     4081 cgagcgcact gtcgcgatag gcatcgtcgc ggtcggccgg ctcgacgaac aactcccgcg
     4141 gaatctcttc catggcgcgc aggaccgcct gatcgctgat cccgcgacgc cgcaggataa
     4201 gctgaaattt catcttttcc gacggatcgt ggttcggggc catattaatc ttttaagaga
     4261 gccccgccgt ctcggccggg tagatccaac attagccact tcccccgcgg gatgactccc
     4321 gttaaaattg aggttccgga catggaaccc gccctatcga cccgcgttaa gggtggggcg
     4381 aaaatcctgt tccaagcctg ccacggtatg tgtgatgtct tcaaccacgt tgacattgcc
     4441 ccggcgggcg ttatttgcga accttgagta atgggacggg caaggacttc ataatgactg
     4501 cagcgcagcc gccgggcggt tcggtgttcc tcgtcgagga cgaggtcatg atcagaatga
     4561 tggtcgccga catgctggag gagcttggct acagcgtcgc ggccgaggcc ggcgagatca
     4621 aagaggcggt caggatggcg caatccgccg agttcgacct cgccattctc gacgtcaacg
     4681 tcaatggcaa ggtaatttcc ccggtcgccg acctgatcaa ggcgcgcaat ctgccgttta
     4741 tctttgcgac cggctatggc tcatcgggcc ttccggaaga atatcgtgac cgtccggcgc
     4801 tgcagaagcc gtttcagctc gagaccttgg ctcggatgat cgacaccgcg ctgaaaagca
     4861 cagcggctta gggccgggta gacgccgcct caccgcagcg ttgcggtcag cgcttccgaa
     4921 aattcatca
//
LOCUS       DASWQN010001066         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1553827, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001066 DASWQN010000000
VERSION     DASWQN010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553827"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..926
                     /locus_tag="VGC82_18255"
     CDS             177..926
                     /locus_tag="VGC82_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEY0425267.1"
                     /translation="MAKSKVHATPTQAMIGLLRDGMTIMAGGFGLCGIPAALIEAIRE
                     SGVTGLTVVSNNAGIDDVGLGVLLASRQIKKMISSYVGENKEFMRQYLAGELEIEFNP
                     QGTLAERIRAGGAGIPAFYTRTGVGTAIADGKDEREFNGQRYIMEHGLFADLAIVHAW
                     KGDTEGNLIYRRTARNFNPNMATAAKVTVAEVEHLVQPGEIDPDHVVTPGIFVQRLVH
                     VPDPDKRIEQRTTRKRTEAAPAMAGTDPLPA"
     gene            936..1604
                     /locus_tag="VGC82_18260"
     CDS             936..1604
                     /locus_tag="VGC82_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HEY0425268.1"
                     /translation="MAWTRDEMAARAAHELVDGMYVNLGIGIPTLVANHIPPGISVQL
                     HSENGMLGIGPFPYEGEEDPDLINAGKQTVTMLPTTSIFDSAQSFAMIRGGHIAISIL
                     GALQVAENGDLANWMIPGKMVKGMGGAMDLVAGVLRCVVLMEHTSRGEPKILKRCALP
                     LTGAGVVSLIVTDLCVLEVDKPGQDGLTLVDMAPGVAVEELRAKTGAQFAVASSLKTA
                     SPAA"
     gene            1664..>2106
                     /locus_tag="VGC82_18265"
     CDS             1664..>2106
                     /locus_tag="VGC82_18265"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0425269.1"
                     /translation="MPSPTLEAAIGGELQETTSTTPEQVHELVAKARVAQAVFETFSQ
                     ERVDAIVRDFAKYVYDNAEAVAAMAHKETGLGVYEDKVQKAKGKSRVIWNNLKGKKSR
                     GIIGEDAEANLILVAKPMGVVGAVCPVTNPVVTPMCNAMFALKAG"
BASE COUNT          439 a          649 c          667 g          351 t
ORIGIN      
        1 attcgcaatt tggttcctct tccctgtgct cgccgttgcg gcgatgtcgt cttgcttttt
       61 gccttgcggt agtctgtgat cccggttatg gactttccgg ttagtccacg tcaaacaaaa
      121 actcggcggc tggcccaaat aagccagacc gccacactga agggaaggcg gcgcgaatgg
      181 cgaaaagcaa agtccacgcg acaccaacgc aagcaatgat tggtctgctg cgcgatggca
      241 tgaccatcat ggcgggcggt ttcggtcttt gcgggatccc cgcggcgttg atcgaagcta
      301 ttcgagaaag cggcgtaacg ggcctcacgg tcgtgtcgaa taacgcaggc atcgacgacg
      361 tcggcctggg agtgctgctc gcgtcccgcc agatcaagaa gatgatctcg tcctatgtgg
      421 gcgagaataa agagttcatg cgccagtatc tcgcagggga gttggagatc gagttcaacc
      481 cacaaggcac gttggctgag cgcatccggg caggaggcgc cggcatcccg gccttctaca
      541 cgcgcaccgg tgtgggcacc gccatcgccg acgggaagga cgaacgcgag ttcaacggcc
      601 agcgctatat catggagcac ggcttgttcg ccgacttggc catcgtgcac gcctggaaag
      661 gcgacaccga aggcaatctg atctatcgcc gcacggcgcg aaacttcaat ccaaacatgg
      721 ccaccgcggc caaggtgacg gtggccgagg tggagcatct ggtgcaaccc ggcgagatcg
      781 accccgacca tgtcgtcacc cctgggattt tcgtgcagcg cctggtgcac gtgcccgacc
      841 ccgacaagcg catcgaacaa cgcaccaccc gcaagcgtac cgaagcagcc ccggccatgg
      901 ccggcaccga tcccctgccg gcataggagg cgataatggc ttggacccgc gacgagatgg
      961 ctgcccgtgc cgcacacgaa ttggtggacg gcatgtacgt gaacctgggg atcggcatcc
     1021 cgacccttgt ggccaaccat atcccgcccg gcatcagcgt gcagctgcac agtgagaatg
     1081 gcatgctggg catcggtccc ttcccctatg agggcgagga agacccggac ctgatcaatg
     1141 ccggaaagca aacggtgacg atgctgccca ccaccagcat cttcgacagt gcccagagct
     1201 ttgccatgat ccgtggcgga cacatcgcta tcagcatcct tggcgctctg caggtggcgg
     1261 agaacggtga cctggcaaac tggatgatcc ccggcaagat ggtgaagggt atgggcggtg
     1321 ccatggacct cgtggccggt gtgctgcgct gcgtggtgct gatggagcac acgtcgcgcg
     1381 gcgaacccaa gatcctgaag cgctgcgcct tgccgctgac aggcgccggc gttgtcagcc
     1441 tcatcgtgac cgacctgtgc gtgctcgagg tggacaagcc tggccaggat ggcctgactc
     1501 tggtggacat ggcaccgggt gtggcggtcg aggaactgcg cgcgaaaacc ggcgcccagt
     1561 tcgccgtcgc atcaagcctc aaaacggcat cgcccgcggc ctagctgccc acgttgtcgc
     1621 aaatccgtcc gcatgtagaa acaaagcaac aaggaggaag acgatgccca gcccgactct
     1681 cgaggccgcg atcggcggag aactgcagga gaccacctcg accacgcccg aacaggtgca
     1741 tgaactcgtg gccaaggccc gggtcgccca agccgtgttc gagacgttct cgcaggaacg
     1801 ggtcgacgcg atcgtccgcg acttcgccaa gtatgtttac gacaacgccg aggccgtagc
     1861 ggctatggcg cacaaggaga cgggcctcgg cgtttacgag gacaaggtgc agaaggccaa
     1921 aggcaagtcc cgcgtgatct ggaacaacct gaagggcaag aagtcccgcg gcatcatcgg
     1981 cgaggacgcc gaggccaacc tgatcctggt ggccaagccc atgggcgtcg tcggcgccgt
     2041 gtgcccggtg accaatcccg tcgtcacccc gatgtgcaac gcgatgttcg cgctcaaggc
     2101 gggcaa
//
LOCUS       DASWQN010001067         4420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1819387, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001067 DASWQN010000000
VERSION     DASWQN010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819387"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..1258)
                     /locus_tag="VGC82_18270"
     CDS             complement(275..1258)
                     /locus_tag="VGC82_18270"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEY0425270.1"
                     /translation="MIKAFVCQDGQLRPLTDTSSVEALRQAVWIDLQDATQDETDRVQ
                     RATGLTVPSEADVSEIETSSRLATRDGTLYLSMPLIRLTDDGARTTSAGFVVSPERLI
                     TVRFAPSRIFDSYAEHLPRGTTLHEGGAHIFVGLLEAIIDRQADALEQVRADLDTISH
                     AIFAMGINQEGGSKREDAILRRTLSQLGHIGDLISHVRDTQVGAARIVPYMEAIAAPW
                     LPKELAPRLVTLRQDIASVSDFDTHLNDKLQFLLDATLGFINMAQNNVMKVMAVASVV
                     GIPPVLIAGIYGMNFKNIPEYDWAWGYAWGWGLIIVTTLIPLGIFRWRKWI"
     gene            complement(1426..2445)
                     /gene="ilvC"
                     /locus_tag="VGC82_18275"
     CDS             complement(1426..2445)
                     /gene="ilvC"
                     /locus_tag="VGC82_18275"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY0425271.1"
                     /translation="MRVYYDRDADVNLIKAKNVAIIGYGSQGHAHALNLKESGVKQLA
                     IGLRGGSAGIAKAETAGLNVMDPADAAKWADVVMVLTPDEGQGDLYREKLGPNMKPGA
                     ALAFAHGLNVHFNLLDPRSDIDVFMIAPKGPGHTVRSEYQRGGGVPCLVAVSQNPSGN
                     ALEVALSYASAIGGGRAGVIETTFKEECETDLFGEQVVLCGGLVELIKGGYETLVEAG
                     YAPEMAYFECLHEVKLIVDLIYEGGIANMNYSISNTAEYGEYVSGPRIITDETKAEMR
                     RVLADIQSGRFARDWMLENKVNQSSFKATRRRLAEHPIEQIGEKLRAMMPWISQNALV
                     DKTKN"
     gene            complement(2510..3025)
                     /locus_tag="VGC82_18280"
     CDS             complement(2510..3025)
                     /locus_tag="VGC82_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425272.1"
                     /translation="MLDQIPRLLRGDAKTPAIDAVVVVLDTTVRDCKKFLSELRSPAA
                     SCNATNLTLFRLAIEEMEAWCLGDRAALRRAYPKAKMRALDRYMQDSICGTWELVAEA
                     VHPGGSRAIHRVGWPLPGQTKHGWADRIGPLLDPEINSSPSFGKLRDGIRRLAATSSS
                     GHPASVGTADR"
     gene            complement(3201..3611)
                     /locus_tag="VGC82_18285"
     CDS             complement(3201..3611)
                     /locus_tag="VGC82_18285"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0425273.1"
                     /translation="MALIEGSRVQNFRSLRDVTLGSLSRNAGAGTLTPLTVVIGKNGV
                     GKSTLFDAFGFLSDSLTVGVEAACDREQRGGFERLRSQGVQEPTRFEVYYREAQNERP
                     SAYELAIDLDDSGRPFVSSEVLLQRRYSGYLASR"
     gene            complement(3662..4180)
                     /gene="ilvN"
                     /locus_tag="VGC82_18290"
     CDS             complement(3662..4180)
                     /gene="ilvN"
                     /locus_tag="VGC82_18290"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY0425274.1"
                     /translation="MPSPTDTTQRSATISVLVDNESGVLARVIGLFSGRGYNIDSLTV
                     APVDAGGMHSRINIVTSGTSMVIEQIKAQLDRLVPVHRVADLTMDGPHVARELALIKV
                     IGTGEQRVEAMRIADTFRARVVDTTLGSFVFELTGNVEKIDAFVELMLDLGLAEVSRS
                     GVAALARGTRVI"
BASE COUNT          856 a         1365 c         1346 g          853 t
ORIGIN      
        1 ttcgagacaa acagaccctg gcccagcata tgatgctcga catcgaccgt cccggctacg
       61 gcaccgtccg gacgctcggt tttcccatga agttctcgga atatccctgg cgcgtccggc
      121 gtcccgcccc aatgctgggt gagcattcag agcacatcgt ggcggaactt gaatacccgg
      181 aagcagaacc tgccgtactg cggcgggacc gggactgcgg cgggaccggg ttacctgacc
      241 tacacaaccc tgttggccaa aggcggcaca ggcttcagat ccattttcgc cagcggaaga
      301 tgcccagcgg tatcagcgtg gtcacgatga tcagtcccca gccccacgca tagccccatg
      361 cccagtcata ctcgggaata ttcttgaagt tcatgccata gatgccggcg atcagcaccg
      421 gcgggatgcc gaccaccgag gccaccgcca tgaccttcat cacgttgttc tgggccatat
      481 tgatgaagcc caacgtggca tccagcagga actgcagctt atcgttcagg tgtgtatcga
      541 aatcgctgac cgacgcgata tcctgccgca acgtcaccag ccgaggcgcc agttccttcg
      601 gcagccatgg cgcggcgatg gcctccatgt agggtacaat ccgagcagcg ccgacctggg
      661 tgtcgcggac atgggagatc aggtcgccga tatggccaag ctggctcaag gtacggcgca
      721 ggatggcgtc ttcccgtttg ctgccgccct cctgattgat gcccatcgcg aaaatggcat
      781 gtgatatggt atccaggtcg gcgcgaacct gctccagcgc atcggcttgc cggtcaatga
      841 tcgcttccaa caggccgacg aagatatgcg caccgccttc atgcaaagtc gtcccccgcg
      901 gcaaatgctc cgcatagcta tcgaagatgc ggctcggggc gaagcggacg gtgatcaggc
      961 gctccgggga gaccacgaaa ccggccgaag tcgttcgtgc accgtcatct gtgaggcgta
     1021 tcaagggcat actgaggtaa agtgtgccgt cgcgggtcgc gaggcggctg gacgtttcga
     1081 tctcgctgac atcggcttcc gacgggacgg tcaaaccggt ggcgcgttgc acccggtcgg
     1141 tttcatcctg cgtcgcgtcc tgcaggtcga tccagacggc ctggcgcagc gcttccaccg
     1201 atgacgtgtc ggtcaacggc cgcaactgac catcttggca aacaaaagct ttgatcatcg
     1261 cgggcccttc gcgaagcggg ggaatgcgag cggcgcggac cggccggatc ggttggcgca
     1321 gcctagcgga aagcgttggg attgtatcgg gccacagcag tcagacgtcc ttggatcgtc
     1381 atggcccggt gccggccgaa ccatgacgag gccaaagtgc aaagatcagt tcttcgtctt
     1441 atcgaccagc gcgttctggc tgatccacgg catcatggcg cgcagctttt caccgatctg
     1501 ctcgatcgga tgttcggcca gacggcgcct ggtcgccttg aagctggact ggttgacctt
     1561 gttctccagc atccagtcac gcgcgaagcg cccggactgt atatcggcca ggacacggcg
     1621 catttcggcc ttcgtttcgt cggtgatgat tcggggcccg gaaacgtatt cgccatattc
     1681 ggcggtattg ctgatcgagt agttcatgtt ggcgatgccg ccttcgtaga tcagatcgac
     1741 gatcagcttc acttcgtgca ggcactcgaa gtaggccatt tccggggcat agcccgcttc
     1801 gaccagcgtc tcgtaaccgc ctttgattaa ctcgaccagg ccgccgcaca gcacgacctg
     1861 ttcgccgaac agatcggtct cgcattcttc cttgaaggtg gtctcgatca cgccagcccg
     1921 cccgccgccg atggcggatg cgtaggacag cgccacttcc agtgcgttgc ccgacgggtt
     1981 ctgcgacacc gccaccaggc aaggaacgcc gccgccgcgt tggtattcgc tgcgcacggt
     2041 atggccgggg cccttcggcg cgatcatgaa cacgtcgatg tcggagcgcg ggtcgagtag
     2101 gttgaaatgc acgttcaacc cgtgcgcgaa cgccagtgcg gcgcccggct tcatgtttgg
     2161 gccaagcttc tcgcggtaca ggtcgccctg gccctcgtcg ggcgtcagga ccatgaccac
     2221 gtcagcccac ttggcggcgt cggcgggatc catgacattc agaccggccg tttccgcctt
     2281 ggcgatgccg gccgaaccgc cgcgcagtcc gatggcgagt tgcttgacgc cgctttcctt
     2341 caggttgagc gcgtgagcgt ggccctgact gccgtagccg atgatggcga cattcttcgc
     2401 cttgatcagg ttcacgtcgg catcccgatc gtagtaaaca cgcatccgtt gatcctttca
     2461 tatttcagtt tgtgttgcgg ttatggccga actaccctgg attatccatt cagcgatcgg
     2521 ctgttccgac ggaagcggga tgacccgagg acgaggtggc ggccagccgc cgtattccgt
     2581 ctcgcaattt gccgaagctg ggagacgagt tgatctcggg gtcaagcagc ggcccgatcc
     2641 tatcggccca tccatgcttg gtctgtccag gcaggggcca tccgactcga tggatcgcac
     2701 gcgagccgcc aggatggacg gcttccgcga ccagttccca agtcccacag atggagtcct
     2761 gcatgtagcg gtccagcgcc cgcatcttgg ccttaggata ggcgcgccgc agcgcggcgc
     2821 gatcaccgag gcaccaagct tccatctcct caatcgccaa cctgaacaga gtgaggttag
     2881 tagcgttgca cgatgcggcg ggcgatcgca gctcgctgag aaatttcttg cagtcacgaa
     2941 cagtggtatc gagcacaacc acgaccgcat caatagcggg agtcttggcg tcgccgcgca
     3001 gcagacgagg gatttgatcc agcaagacac gcttcgaggg attagccgat gtcgataagt
     3061 ctgtcggaat gcgaccgatc ccgcggtacg catgggagcg ccacgaatga ggctcgccat
     3121 cggtccgatg agttttggca tcagatgctc aagaagacgt cgaccggagt tgtcctcggt
     3181 tagaatctca atatgcattt ctatctcgag gcgagatagc cgctgtaccg gcgttgcaac
     3241 aacacctcgg acgagacaaa tggccgtccg gagtcgtcga gatcgatcgc caactcatag
     3301 gcactcgggc gctcattctg cgcctctcga taatagacct caaatctagt cggctcctgg
     3361 acaccctggg agcggagccg ctcgaaacca cctcgctgct ctcggtcaca cgccgcttcg
     3421 acaccgacgg tcaacgagtc ggacagaaag ccgaacgcat cgaacaaagt gctcttgcca
     3481 acgccgtttt tgccgatcac cactgtcaac ggggtgagag tgcctgctcc ggcattgcgc
     3541 gacagcgatc cgagcgtgac gtcgcgcagc gatcggaagt tctgaacgcg ggagccctct
     3601 atcagtgcca catcgaccct tccgactcgt tcaggcgcgc gtcattatcg cggcggcgcg
     3661 ttcaaatcac ccgagtgcca cgggcaagcg cggcgacgcc actgcgtgaa acctcggcta
     3721 agccaagatc aagcatgagt tcgacaaagg cgtcgatctt ctcgacattg ccggtcagtt
     3781 cgaacacaaa actacccagc gtcgtatcga caacccgggc gcggaacgta tcggcgatgc
     3841 gcatggcctc gacccgctgc tcgccggtgc cgataacctt gataagcgcc agttcgcggg
     3901 caacatgcgg cccgtccatc gtcaggtcgg cgacgcggtg caccggtacc agacgatcga
     3961 gctgtgcctt gatctgctcg atcaccatgc tggtgccgga ggtgacgatg ttgatgcggc
     4021 tatgcatccc gccagcatcg accggtgcga ccgtcaggct gtcgatgtta tagccgcggc
     4081 ctgaaaacaa cccgataacg cgggcaagca cgccggactc gttgtccacc agcactgata
     4141 tcgtcgcgct gcgttgggtc gtatccgtgg ggctgggcat gcggtgaacc aggctgtggg
     4201 gaaaaggatg gcaaacaagt gtagggcgcg ggcgggtcat gccccgccgc gtccggatag
     4261 gcaagaggca gatgccgtca gaccagcgcc atgcccgcgt cggtgatgcc ggcgccggag
     4321 gcttcatgct ctggacccag cagcatttcg ttgtgcgcgg cgccgctggg gatcatcgga
     4381 aagcagtttt ccgccgggtc cacggcgatg tcggcgatca
//
LOCUS       DASWQN010001068         4494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1553898, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001068 DASWQN010000000
VERSION     DASWQN010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553898"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VGC82_18295"
     CDS             complement(<1..381)
                     /locus_tag="VGC82_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462362.1"
                     /note="with YejAEF is involved in resistance to microcin
                     C; Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin ABC transporter permease"
                     /protein_id="HEY0425275.1"
                     /translation="MASYLLRRLLLVIPTLFGIIAINFMVVQFAPGGPVEQMIAELKG
                     GGSVTGRMTGAGAAETMPIGDGSYRGARGLDPHVLADIRRMFGFDKPAPERFVLMMRG
                     YLTFDFGRSFFRDKPVLQLVEEKLP"
     gene            complement(440..2314)
                     /locus_tag="VGC82_18300"
     CDS             complement(440..2314)
                     /locus_tag="VGC82_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0425276.1"
                     /translation="MRMIWGLGLSLWLLAGTGLSLAQAPAVPAQGALKPAAEAPLRTH
                     AVSLLGQPSLPPDFKFFPYVNPNAPKGGDVAISSVGTFDSFNPFIVRGNAPMDVQRVW
                     DTLMKTNADEAETEYGLLADVIELPADRMGVAFELRPEAKFHDGTPVTAEDVAWTFET
                     LRDKGRPFYRQYYADVASVTVEGPRRVVFRFTSNSNRELPLILGQMAVLPKHWWAGRD
                     FDKPLTDPPLGSGPYRVGHFEFGRSLSLERVPDVWSKDLPVMRGLANFDTRRTEYFRD
                     GTVALEAFKAGQIDFREENVAKDWATAYDFPAVQKGLVKKELLPHRLPTGMQGYAMNT
                     RRALFKDVRVRHALALAFDFEWANANLVYGSYVRTNSYFSNSDLASSGIPQGAELELL
                     NKYRDQLPADIFTKPFRLPVTDGSGNNREEMRNALALLEQAGWKVRDRKLVDAQGQPF
                     TFEILLDQPAFERVSLPYVQWLSRLGIEARVRTVDPAQFQRLLDTYDYDMIVVTIGAS
                     ESPGNEQTGYWTCDSLKPEGGFNLMGVCNPVVDDLVHRVVSASDRAELVVAAHALDRV
                     LLAGWYVVPHWHLQSVRAAYWDRFGKPDKPVRTGIAFDSWWLDPVRAAANDAARRNGP
                     "
     gene            complement(2586..3548)
                     /locus_tag="VGC82_18305"
     CDS             complement(2586..3548)
                     /locus_tag="VGC82_18305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY0425277.1"
                     /translation="MQIGFNAPTSGPLIDPDSLTRIITEGEALGFDYVTISDHIMVPR
                     NLESKYPYTDTGEFPAGTQAAWLEQLATTAYIAALTKKLRFVLSVMVVPHRPAVLTAK
                     LLSTIDFLSKGRLTLGIGVGWCREEFEAIGAAPFDDRGHVTDEWMDVCKELWTADLPK
                     FSGKYVKFDDVVFTPKPVQNPIPIWVGGESGPALRRTVKYAHGWYPVGTNPQFPMNTP
                     NRFKAGLARFRGFAEKAGRDPSEIKIALRVLAGPGVKPRGSIGGEAEMFTGSNADWVA
                     DINVLVELGVHAVDVRLFGYGPNQSLDATIDNMRRFRDGVLIKL"
     gene            3746..>4494
                     /locus_tag="VGC82_18310"
     CDS             3746..>4494
                     /locus_tag="VGC82_18310"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0425278.1"
                     /translation="MRQIRIGDITIDAVVEREGPWRRPQDFFPAYDDATFKRHLPAME
                     PEVFDPALGMMFITYQTFVVRTPRHTILVDTCTGEEKGHPPPFDFPGKERWRNELFAL
                     GVGYDKIDYVFCTHLHIDHTGWNTTLRDGRWVPTFPNAKYIFHKKEYAAWEAEHAKGA
                     NPPGTVFRDNCLPIVEAGQALLVDDDYALDDTITLTPTPGHSPCHCCVNIASKGQRAS
                     VTGDMMHHAIQCRELDWSPGVDWDPKEAAVSR"
BASE COUNT          853 a         1429 c         1385 g          827 t
ORIGIN      
        1 aggcaatttc tcctcgacga gctgcagcac cggcttgtcg cggaagaagc tgcggccgaa
       61 atcgaacgtc aggtagccgc gcatcatcaa tacgaaacgt tcgggggcgg gcttgtcgaa
      121 gccgaacatg cgtctgatgt cggccagcac atgcgggtcc agaccgcggg cgccgcgata
      181 gctgccgtcg ccgatcggca tcgtttccgc cgcgccggcg ccggtcatgc gcccggtcac
      241 gctgccgccg cccttcaact cggcgatcat ctgttcgacc ggaccgccag gggcaaactg
      301 caccaccatg aagttgatgg cgatgatgcc gaacagcgtc gggatgacca gcagcaggcg
      361 gcggaggaga taggacgcca tcagcctctt gtgccgcctg gcgtgccggt cacggcgatt
      421 cggggcaacg tcgaaagcgt catgggccgt ttcgccgcgc ggcatcgttc gccgcggctc
      481 gtaccggatc cagccaccag ctatcgaagg cgatcccggt tcgcaccggc ttgtctggct
      541 tgccgaagcg gtcccaatag gcggcccgca ccgattgcag atgccaatgc gggaccacgt
      601 accaacccgc cagcagaacc cgatcgagcg catgcgccgc gaccacgagt tcggcgcgat
      661 ccgacgcgga aaccacccgg tgcacgaggt catcgacgac cgggttgcag acccccatca
      721 ggttgaagcc gccctcgggc tttaagctgt cgcaggtcca atatccggtc tgctcgttgc
      781 cggggctttc agacgcgccg atcgtgacta cgatcatgtc gtagtcatag gtgtcgagca
      841 gccgttgaaa ctgggccggg tcaacggtgc gcacgcgcgc ctcgatcccc agccgtgaca
      901 gccattgtac atacggcagc gatacccgtt cgaacgccgg ttgatccagc aggatttcga
      961 acgtgaatgg ctgaccttgg gcatcgacca attttcggtc acgtaccttc cacccggcct
     1021 gctccagcag agccagtgca ttgcgcattt cctcgcggtt gttgccggac ccgtcggtca
     1081 ccggcaaccg gaatggtttg gtgaaaatat ccgccggtag ctgatcgcga tacttgttca
     1141 gcaattccag ttcggcgccc tgaggaatac cgctggaagc gaggtcgctg ttgctgaaat
     1201 agctgttggt acggacataa ctgccataga ccaaatttgc attggcccac tcgaaatcga
     1261 acgccaatgc cagcgcatgc cgcacccgca catccttgaa cagcgcccgg cgggtgttca
     1321 tggcgtagcc ctgcatgccc gtgggcagcc gatggggcag caattccttt ttcaccaggc
     1381 ccttttgcac cgcggggaag tcgtaggctg tcgcccagtc cttggcgacg ttctcctcgc
     1441 ggaagtcgat ctgccccgcc ttgaacgctt ccagcgcgac cgtcccatcg cggaaatatt
     1501 cggtgcggcg ggtatcgaaa ttcgccagcc cccgcatcac cggcagatcc ttcgaccaga
     1561 cgtcgggcac gcgctccagc gacaggctgc ggccgaactc gaagtggccg acccggtagg
     1621 ggccggaacc gagcggcgga tcggtcagcg gcttgtcgaa gtcccggccg gcccaccaat
     1681 gttttggcag caccgccatc tggccaagaa tcagcggcag ctcgcggttg gagttggacg
     1741 tgaagcggaa gacgacccgg cgcggccctt caaccgtgac cgatgccacg tcggcgtagt
     1801 actgccgata aaatgggcgg cccttgtcac gcaaagtttc gaaggtccag gcaacgtctt
     1861 cggcggtgac cggcgtgccg tcgtggaact ttgcttccgg ccgcagttcg aaggcgacac
     1921 ccatgcggtc ggccggcaac tcgatcacgt cggcaagcag gccatactcg gtttcggcct
     1981 cgtcggcgtt ggtcttcatc agtgtgtccc agacccgttg cacgtccatc ggcgcattgc
     2041 cgcgcacgat gaacgggttg aagctgtcga aggtaccgac ggagctgatc gccacgtcgc
     2101 cgcctttcgg agcgttggga ttcacatagg ggaaaaattt gaagtccggc ggcagcgatg
     2161 gttgccccag cagcgaaacg gcatgggtgc gcagcggcgc ctcggctgcc ggcttcaagg
     2221 caccttgggc gggtacagca ggcgcctgag ccaggctcaa ccccgttccg gcaagcagcc
     2281 agagtgacag ccccaatccc cagatcatgc gcatcgctcg actccaatgt cgtcgtcatg
     2341 gcagctgtcg ttgtcatggc agcggcgacc gcccgcgttg ctgcctcggc tccattgagc
     2401 tgccttaaac ccatcgagct gcctcaaaca atgtcatcga aggcgcgccg ggcggcaatc
     2461 gccgctagca gcctccgcca gttcgaacca gtggtttaac gttaaaatcc gtccaggcgc
     2521 cggccgtgcc cctgctccga cgcgccacct ggcgccgcag tctcgaaaac cggccaccat
     2581 tgcggtcaga gcttgatcag cacgccgtca cggaagcggc gcatgttgtc gatggtcgca
     2641 tccaggctct gatttggacc atagccgaac aatcggacat cgacggcatg cacgccaagc
     2701 tcaaccagca cgttgatgtc cgcaacccag tcggcgttgc tgccggtgaa catttcggct
     2761 tcgccgccga tcgagccgcg cggcttgaca ccgggacccg ccagcaccct gagagcgatc
     2821 ttgatttccg acggatcacg gccggctttc tcggcgaacc cgcgaaaccg cgccagtcca
     2881 gccttgaagc ggttcggcgt attcatcggg aattgcgggt tagtaccgac cggataccag
     2941 ccatgggcat acttgacggt tcgccgcagt gccggcccgc tttcgccacc gacccagatg
     3001 ggaatggggt tctgtaccgg tttcggcgtg aacaccacgt cgtcgaattt gacatacttg
     3061 ccggaaaact ttggcaggtc ggccgtccaa agctccttgc agacgtccat ccactcgtcg
     3121 gtgacgtgcc cgcggtcgtc gaacggggcc gcgccgatgg cctcgaactc ctcccggcac
     3181 cagccgaccc cgataccgag cgtcagacgg cccttggaga ggaagtcgat ggtggacaaa
     3241 agcttggcgg tcagcacggc cggccggtgc ggcacgacca tcaccgacag cacgaagcgc
     3301 agcttcttgg tcagcgcagc gatataggcg gtggtagcga gttgctccag ccacgcggcc
     3361 tgcgttcccg cgggaaattc gccggtatcg gtatagggat atttcgattc gaggttgcgg
     3421 gggaccatga tgtggtcgct gatcgttaca tagtcgaagc cgagtgcctc gccctcggtg
     3481 atgatgcggg tcaggctgtc cggatcgatc aacgggccgg atgtaggcgc gttaaatccg
     3541 atctgcataa cgttttcccc aatgttgttg cctcatgaaa tcagaagctc ccgggattga
     3601 gaagggaccc ccccttgatg tcggccgatc atggtcggca accccacctt gggatcccgc
     3661 caccgggatc ccagcaccgc cttcagggcc ttttcgtggc gccgagtgct tgttacgctg
     3721 cgccggtcaa gcgggggagg cagcaatgcg gcaaatcagg attggcgaca tcacgatcga
     3781 cgcagtggtc gaacgggaag ggccgtggcg gcggccacag gattttttcc cggcctacga
     3841 cgacgccacg ttcaagcggc atctgccggc gatggagccc gaagtgttcg atccggcgct
     3901 ggggatgatg ttcatcacct accagacctt cgtggtccgt acgcctcgcc acacgatcct
     3961 ggtggatacc tgcaccggtg aggaaaaggg ccacccgccg ccgttcgatt tccccggcaa
     4021 ggagcgttgg cggaacgagc tgtttgccct cggcgtcggc tacgacaaga tcgattacgt
     4081 gttctgcacg cacctgcaca tcgaccacac cggctggaac accaccctgc gcgacggacg
     4141 ctgggtgccg acgtttccga atgcgaaata catctttcac aagaaggaat acgctgcctg
     4201 ggaagcggag catgcaaagg gcgccaatcc gccaggcaca gtgttccgcg ataactgcct
     4261 gccaattgtc gaagccggac aggcgctgtt ggtggatgac gattatgcct tggacgacac
     4321 catcacattg acaccgacgc cgggtcactc gccctgccat tgctgtgtca atattgcgtc
     4381 gaagggtcag cgcgcctcgg tgaccggcga catgatgcat catgcgattc agtgccggga
     4441 gcttgactgg tcgcccggtg ttgactggga cccgaaagag gcagcggtct cgcg
//
LOCUS       DASWQN010001069         3398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1026904, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001069 DASWQN010000000
VERSION     DASWQN010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026904"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VGC82_18315"
     CDS             complement(<1..586)
                     /locus_tag="VGC82_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0425279.1"
                     /translation="MIKLISWNLLRLTGASLDDVVRLIRREQPGLLLMQEVTQEMDGL
                     TARVGGQYYRNLMPGRIHGLAVWSPTAFAGAPVVLPLQSGAMFDRICQIVDLGSISVA
                     NVHLSHGQILNRRQLRRIAKVLPPRAAVVGDYNLIGPALLPGFHDVGPREPTHVAGDV
                     MPLRIDRCLIRGVTCLGARALLREASDHRPIVVNL"
     gene            complement(981..1829)
                     /locus_tag="VGC82_18320"
     CDS             complement(981..1829)
                     /locus_tag="VGC82_18320"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0425280.1"
                     /translation="MGPNLVDYLLYEPEDSGILWIRFNRPERMNALVGTAEENGTVAK
                     VGEYMRAGDDDPNIRVIVLTGVGRGFCSGANLASKASGDVTGQNFPGNRGSHEGPDAA
                     RQHFFHGFTALHRDIALIRKPTIAMINGPAVGSGMDMALHCDVRVGCEKTRFIGYHNA
                     GQIIENGGSYYLPKMVGLGRALEFAYTGELDAERAFAWGLLNHLVPLEELEATTRALC
                     QKMMGIPPLVQWVSKRVMRAALDSTLETTMVLTSNAGGILQNSEDATEARRAFVEKRK
                     GRFKGR"
     gene            complement(1887..>3398)
                     /locus_tag="VGC82_18325"
     CDS             complement(1887..>3398)
                     /locus_tag="VGC82_18325"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0425281.1"
                     /translation="AQVTWSSTLSPSSWNGVTTVLLGNCGVGFAPCRPDQRDMLVKLM
                     EGVEDIPEVVLTEGLPWTWQTFPDFLDVLETRRYDADVAAQVPHAALRVYVMGQRGAD
                     REPATEQDRRRMAELAEEGIRAGALGFSTSRTLNHRTLDGKHIPTLRAEEAELTAVAH
                     AMRSAGAGWLQIVSDFEDQDNEFGLFRRLAEASGRPVTLTLLQSDTKPDGWRDLLGQI
                     EAANAAGLRITGQVRSRPTSVLLGFELSQNPFMGRPSYKKIAGLNFQNRMRHLRDPAF
                     RAVLLAEAFQGGRRAQRVERWDRMFPLGDPPDYEPSPEDSVAARAAREGRSPDEVAYD
                     LLMERDGKAILYLPVTNYAAGNLDVVREMIAHPNTLLGLGDGGAHVGIMCDATATSYT
                     LTHWTRERERGALFPVAWAIRRLSRDNAAAIGLDDRGLLRVGMKADLNVIDYDRLVLR
                     SPEIVYDLPAGGKRLVQRTDGIDATIVSGALVYRNGAATGALPGRLVRGPQTG"
BASE COUNT          629 a         1120 c         1030 g          619 t
ORIGIN      
        1 taagattgac cacgatcggc cgatggtcgg atgcctcccg cagcagcgct ctggctccca
       61 ggcatgtaac gcctcggata aggcaccggt cgatccgcag cggcatcaca tcgcctgcga
      121 cgtgcgtcgg ctcgcgcggc ccgacatcat gaaaacccgg caggagcgcc gggccgatca
      181 ggttgtagtc gccaaccacc gcggctcggg gtggcagaac cttggcgatg cgtcgaagct
      241 gccggcggtt caggatctgg ccatgcgaga ggtggacgtt ggcgacgctg atagaaccca
      301 ggtcaacgat ctggcagatc cggtcgaaca tggctccgga ctgcagtggc agcacgacag
      361 gtgcaccggc gaatgccgtc gggctccata ccgccaaccc gtggatgcgg ccgggcatga
      421 ggttgcggta gtactggccg cctactcgtg cggtcagacc gtccatctcc tgcgtgacct
      481 cctgcatcag gagcagacca ggctgctccc gccggatcaa gcggacgaca tcgtcgaggg
      541 aggcccctgt taggcggagc aagttccaac tgataagttt gatcattgca accgatgaat
      601 agacgaaacg gttagccagg cgcaaccact gtctgcaggg tcggcgccca ttggcaccag
      661 gaccgtccac ccatcacacg accgactatc gctactccgc atgaacctcc ccggccgtaa
      721 aatcaacgca ggaggatcaa gcccgatttc cgccgccgtt accacctcgg tcttgatcgg
      781 cctgaactgc cggtgtgctc gcgctttttc ccagcatcgg cacttgccgc gaggcaacgg
      841 aaaatgacgc ccagcattga tcgcagcatc agccatccag caccccgtgg ctccgccaca
      901 agatataaga actatctgcg gtattcaacg catcggccgg cataaccaac acagcctagc
      961 caacattgcc tcagcgcgaa ttagcggccc ttgaagcggc ctttccgctt ttcgacgaag
     1021 gcccgtctcg cctctgttgc gtcctccgaa ttctgcagga tgccgccggc gttcgaggta
     1081 aggaccatgg tcgtctccag cgtgctgtcc aaggcggcac gcatgacgcg tttgctgacc
     1141 cactgcacca acggcggaat tcccatcatc ttttggcata gcgcccgagt ggtcgcctcc
     1201 aattcttcca gcggtaccag atggttcaac agcccccagg caaacgcccg ctccgcgtcc
     1261 agctccccgg tataggcaaa ctccaaggct cggccgaggc cgaccatctt gggaagatag
     1321 tatgagccgc cgttttcgat gatttgcccc gcgttgtgat agccgatgaa gcgggttttt
     1381 tcgcacccca cgcgcacgtc gcaatgcaat gccatgtcca tcccggagcc gaccgccggc
     1441 ccgttgatca tcgcgatggt cggtttgcgg atcagtgcga tatcccgatg cagggcggtg
     1501 aaaccgtgaa agaaatgctg gcgcgcggca tcgggccctt cgtgcgatcc gcggttgccg
     1561 ggaaaattct gcccggttac atcgcctgat gccttggatg cgaggttcgc cccggaacaa
     1621 aagcctcgtc ccacgccggt cagcacgatg acccggatgt tgggatcgtc gtcaccggcc
     1681 cgcatgtatt cgccaacctt cgccacggtg ccgttttctt cggcggtccc gaccaaggcg
     1741 ttcatccgct ccgggcggtt gaacctgatc cagaggatgc cactgtcctc cggctcatag
     1801 aggaggtagt cgaccagatt tggtcccatc ggttgcttcc accctttata ttgttcgcgc
     1861 gagaagtctc ctgtcgggca agcccttcaa ccagtttggg gcccccgcac aaggcgccct
     1921 ggcaaggccc cggtcgccgc gccgtttcga tataccagcg ccccggacac gatggttgcg
     1981 tcgataccgt cggtccgctg caccagccgc ttgccgcccg ctggcaggtc atagacgatt
     2041 tccggactgc gcagcaccag gcggtcgtag tcgatcacgt tcaggtccgc cttcattcct
     2101 acgcgcaaca gtccgcgatc gtccaagccg attgcggcgg cgttgtcgcg gctgagccgc
     2161 cttatcgccc aagccaccgg gaacagtgca ccgcgctcgc gctcgcgggt ccagtgcgtg
     2221 agcgtgtagc tggtcgccgt ggcatcgcac atgatcccga catgcgcgcc cccatcgccc
     2281 agccccagaa gcgtgttcgg gtgcgcgatc atctcccgca cgacgtccag attccccgct
     2341 gcgtagttgg tgaccggaag gtagaggatc gccttgccgt cacgctccat caaaaggtca
     2401 taggcaacct catcgggcga gcgtccttcc cgcgccgccc gggccgcgac gctgtcctca
     2461 ggcgagggtt cgtagtccgg cgggtcgccc aaagggaaca tgcgatccca gcgctcgacg
     2521 cgttgggcac ggcggccacc ctgaaacgct tccgccagca gcaccgcacg gaaggccgga
     2581 tcacgaaggt gccgcatccg attctggaag ttcagtccgg caatcttctt gtagctcggc
     2641 ctgcccatga acgggttctg cgacagttcg aaacccagca ggacactggt ggggcgggag
     2701 cggacctggc cggtgatgcg caacccagct gcatttgcgg cttcgatctg gcccagcaga
     2761 tcacgccacc catccggctt ggtgtccgac tgcagcaggg tcagcgtgac cggccggccg
     2821 gacgcttccg cgagccggcg gaacaggccg aactcgttgt cctggtcctc gaagtccgaa
     2881 acgatttgca gccatccggc gccggcggag cgcatggcat gcgcaacggc cgtgagttct
     2941 gcctcctcag cgcgcagagt agggatgtgc ttgccgtcga gggtgcggtg attgagcgtg
     3001 cgggatgtgg aaaaaccgag tgccccggcg cgaatgcctt cctcggccag ttcggccata
     3061 cggcggcgat cctgttcggt cgcgggttcc cggtccgctc cgcgctggcc cataacgtaa
     3121 acccgcaacg ccgcgtgtgg gacctgggcg gccacgtccg cgtcgtaacg ccgagtctcc
     3181 aacacatcga gaaagtcggg aaaggtttgc cacgtccagg gcaggccctc ggtcaagaca
     3241 acctcgggga tgtcttcgac accttccatc agcttgacga gcatgtcgcg ttgatcggga
     3301 cggcaggggg caaagccaac gccgcagtta ccgagcagaa cggtggtgac gccgttccac
     3361 gaggatggag acagggttga actccatgtc acctgggc
//
LOCUS       DASWQN010001070         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1686953, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001070 DASWQN010000000
VERSION     DASWQN010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686953"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..>1595
                     /locus_tag="VGC82_18330"
     CDS             111..>1595
                     /locus_tag="VGC82_18330"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0425282.1"
                     /translation="MSEAIEDIRSAGTTDVKNIDVAIVGGGLGGLYALHRLRGLGLSV
                     KVFEAGSGVGGTWFWNRYPGARCDVESMEYSYGFSNELQQEWTWPERYGTQPEILRYI
                     NHVADRFDLRRDIALNTRVVSAVFDPSAGRWTVKTDQGDTVTARFCVMARGNLSTPRV
                     PDFRGLTTFQGKWYHSGLWPHEGVDFSGLRVGVIGTGSSGVQMIPNIAAQADHLTVFQ
                     RTANFSLPARNVPMDPEKERRHKQEYEDRRRAALDTPFGIAGHPAPTQSALEATEEER
                     QRAYERKWQEGGSISFLYSYTDLLVNQAANDTASEFVRQKIRSIVRNPATAEILAPKD
                     HPIGTKRLCLDTNYYETYNRNNVSLVDVKHDPIQEITATGLRTRDQTYGPFDALVFAT
                     GFDAMTGALREIDIRVKDGPALADQWEAGPRTYLGLMVSGFPNMFVVTGPGSPSVKTQ
                     MILAIEQHTDWIADCLTYLRANGYSRIEASRPAQDEWVDHVNKVA"
BASE COUNT          314 a          497 c          503 g          281 t
ORIGIN      
        1 cctgatcctg ctcgctttgt cccgccggtt caatgcgacg gacaaaggct tgacagggag
       61 tgtgaaaccc ggacccttcg catggtccgc agccaacgag agccagcgtc atgtccgaag
      121 ccattgaaga catcaggtcc gccgggacga cggatgtgaa aaacatcgac gtcgccatcg
      181 taggcggcgg gcttggcggg ctttacgcat tgcaccgcct gcgcgggctg ggcctgtcgg
      241 tgaaagtgtt cgaagccggg tccggcgtgg gcggcacatg gttctggaat cgctaccccg
      301 gcgcccgctg cgatgtcgaa agcatggagt actcctatgg cttttccaac gaactgcagc
      361 aggaatggac ctggccggag cgttacggca cccagccgga gatcctgaga tacatcaatc
      421 atgtcgccga ccggttcgac ctgcgccgcg atatcgcgct gaacacccgc gtggtgtccg
      481 ccgtgttcga cccttcggcc ggccgctgga ctgttaaaac cgatcaaggc gacaccgtca
      541 ccgctcggtt ttgcgtcatg gcccgcggca acctgtccac cccccgggtg cccgatttcc
      601 gcgggctgac gacgttccag ggcaaatggt atcacagcgg cctgtggccg cacgagggcg
      661 tggatttctc cggactgcgc gtgggcgtga tcggcaccgg gtcgtccggc gtgcagatga
      721 tcccgaatat cgcggcacag gcggatcacc tgacggtctt ccagcggacc gccaatttca
      781 gcctgcccgc gcggaatgtg ccgatggatc cggagaagga gcgtcgccac aagcaggaat
      841 acgaggatcg ccgtcgggcc gcactcgaca ccccgtttgg gattgccggc catccggcgc
      901 cgactcaatc agcgcttgag gcgaccgagg aagagcgcca gcgcgcctat gagcggaaat
      961 ggcaggaagg cggcagcatc agcttcctgt actcctacac cgatctgctg gtgaaccagg
     1021 cagccaacga caccgcgtcg gagttcgtgc gtcagaagat cagaagcatc gtccgcaatc
     1081 ccgcgacggc ggagatcctg gcgccgaagg atcatccgat cgggaccaag cggctgtgtc
     1141 tcgatacaaa ctactacgag acctataatc gcaataacgt ttctttagtg gatgtgaagc
     1201 acgatccgat ccaggagatc accgcgaccg ggttgcgaac tcgcgaccaa acctatgggc
     1261 cattcgacgc gttggtgttc gccaccggct ttgatgcgat gaccggagcc ctgcgggaga
     1321 tcgatatccg cgtgaaggac ggccccgctc tggccgacca gtgggaagcc ggtccacgta
     1381 cctatttggg cttgatggtc tcgggcttcc ccaacatgtt cgttgtcacc ggcccgggca
     1441 gcccgtcggt gaagacgcag atgatcctcg ccatcgagca gcacaccgac tggatcgccg
     1501 actgcctgac ctacttgcgt gcgaatggct acagccggat cgaggcgagc cggccggcgc
     1561 aggacgaatg ggtggaccac gtcaataagg tcgcc
//
LOCUS       DASWQN010001071         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2612046, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001071 DASWQN010000000
VERSION     DASWQN010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2612046"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VGC82_18335"
     CDS             complement(<1..251)
                     /locus_tag="VGC82_18335"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0425283.1"
                     /translation="MQMRIIWGKILPGQWDAFEAAFKRTLEIRGEVKGLQGQWLLRDE
                     NDPDAGYSISQWESAEDMRAFWDSKKRNEAMAVLQPFYV"
     gene            527..1309
                     /locus_tag="VGC82_18340"
     CDS             527..1309
                     /locus_tag="VGC82_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0425284.1"
                     /translation="MAIRKVLLPLISTGAGEAALSTAWTIARLWNAHMSVLHVQMDLA
                     PAPAMSAMFRRFTVEHQVKVAEATAAAEGATASFLILTGRRLDILPQQARLADLTVVR
                     HPATPEEVSSSDTLHAVLFDSGRPLLIAPQVAPPTVGRRICLGWNGSAESAAAVEAAM
                     PWLQRAEAVRILWAEEYQRRGPQAPELQAYLAMHGVPADLKMFKPIDKVVGAGLLAAA
                     NEFNCDLLTMGAYAHSRLRQQILGGVTRHILEHATLPVMMHR"
     gene            1718..2119
                     /locus_tag="VGC82_18345"
     CDS             1718..2119
                     /locus_tag="VGC82_18345"
                     /inference="COORDINATES: protein motif:HMM:NF024980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0425285.1"
                     /translation="MNALLLLDDGWAAERVADVLFIDAETVREHRRRYQVGGVAGVEQ
                     LKFEGSDPALSPEQLKTLGAELDAALDASAKEICAFVQRSFAVSDTPHAMAKLLKRLG
                     FVYKKPKCVPAKANEPAQRAFVAEILDGAGQ"
BASE COUNT          433 a          678 c          681 g          469 t
ORIGIN      
        1 ttcacgtaga acggctgaag cacggccatg gcctcattgc gtttcttgct gtcccagaac
       61 gcccgcatat cctccgcact ttcccactgc gagatcgaat atcccgcatc ggggtcattc
      121 tcgtcccgaa ggagccattg tccttgcagc cccttcactt cgccacgaat ttctagcgtt
      181 cgcttgaagg cggcttcgaa agcatcccat tggccgggca ggattttgcc ccaaatgatc
      241 cgcatctgca tcgcaatacc ccctatcggc actcggcaag gtacgtcgca ggcgaccacc
      301 ggacattgcc cggatcgctc tgttagccca agtgtccgcc gataagatta ccggcagcgg
      361 cgtgcgttta caaacaaccc gcgaagttcg cccgggccgg gtgagatgga caggcgcagc
      421 acaggctaca aacatttcag acacctcatg cgcttctgac aaggtcgttg acacgttcta
      481 tttggctttt atcctccaaa agagccttca tcggagcgtt gggatcatgg ccatccgcaa
      541 ggttttgttg ccgttgatca gcactggtgc gggagaggca gctttatcca ccgcctggac
      601 gatcgcccgg ctctggaatg cgcacatgtc cgtactccat gtgcaaatgg acctggctcc
      661 ggccccagcg atgagcgcaa tgttccgccg cttcactgtt gagcaccaag tgaaagttgc
      721 ggaagccact gctgcggctg aaggcgccac cgctagcttt cttatcttga cggggcggcg
      781 gctggatatt ctcccgcagc aggcgcggct cgccgatctt accgtggtgc gtcatcctgc
      841 caccccggaa gaggtgtcct cctccgatac tctgcacgcg gtgctgtttg attccggtcg
      901 tcccctgttg attgctcccc aggtcgcgcc gccgacagtt ggcaggcgga tatgccttgg
      961 ttggaacggc tctgctgagt ctgccgccgc ggtggaggcg gccatgcctt ggctgcagcg
     1021 cgcagaagct gtgcgcattc tctgggccga agaataccag cgacgaggtc cgcaggcgcc
     1081 agaacttcaa gcctatttgg cgatgcatgg cgtgcctgct gatttgaaga tgttcaaacc
     1141 aattgacaaa gtagtaggag ctggtctgct ggcggcggcc aacgagttca actgcgatct
     1201 gctgaccatg ggtgcctatg cgcactcccg tctgcggcaa cagattcttg gcggcgtgac
     1261 gcggcacatc ctggagcacg cgaccttgcc ggtgatgatg catcgatagc cgggcaagcc
     1321 cggctttctc cgtcgagatc gcgccgcgga aaaccttcgg agttgaggtc aggtcggggc
     1381 tttcggttat ggttttcttc gtggtcaacg cggccgaatt ccatccctta cccatgccag
     1441 tcatatataa accggattgc acgggctaat tggggccgct cggccaccag cgccagaacg
     1501 gccatgctga attggatgag gcgtttttcc ggcgaacggt tcgcgtgcgc ggttgtggta
     1561 taccccgact ccgcgagatt ccgtgcgatg cctctttaca aagccagttg agttcgattc
     1621 gctgtctggc atgagcggtt cgattctcac ccccgaggac cgggcgcact tccttcggaa
     1681 gatgcgtcat ctgacaccca gcctggttca tcgccggatg aacgcgctgt tgctgctgga
     1741 cgacggctgg gccgcggagc gtgtcgcgga cgtgctgttc atcgacgccg aaacggtgcg
     1801 cgaacatcgg cggcggtatc aggttggtgg tgtggccggt gtcgagcagc tgaaattcga
     1861 gggtagcgat ccggccctga gcccagagca actcaagacg ctgggagcgg aactggacgc
     1921 tgcgctcgac gcgtcagcga aagagatatg cgccttcgtg cagcgcagct tcgccgtgag
     1981 cgacacgccg cacgcaatgg ccaagctgct gaagcggctg ggctttgtct acaagaagcc
     2041 gaagtgcgtg ccggcaaaag ccaacgagcc ggcacaacgg gcgttcgtgg ccgagatcct
     2101 tgatggcgca ggccagtgac aagaccccat tgtatttcgt tgacgggacg catccctccg
     2161 acaccgcgca cgccgcgtat ggatggatcc gcaaaggcca gacgtgcgaa ctgaagagca
     2221 accacggccg gacgaacatc aacatcaatg gggcgttgag c
//
LOCUS       DASWQN010001072         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_762440, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001072 DASWQN010000000
VERSION     DASWQN010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762440"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="VGC82_18350"
     CDS             <1..1312
                     /locus_tag="VGC82_18350"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY0425286.1"
                     /translation="ASDTYALFGTNFPAIEVMAFLLFIGAMGKSAQIGFHVWLPDAME
                     GPTPVSALIHAATMVTAGVFLMARMSPLMEYAPVALDFVTFIGATTALFAATIGCVQN
                     DIKRVIAYSTCSQLGYMFVAAGVGVYQASVFHLITHAFFKALLFLSAGSVIHAMSDEQ
                     DMRKMGGIWKFIPVTYAVMWVGSLALAGVFPFAGYYSKDAIISGAYAAHSGIGTYGFI
                     CTVLAAFLTAFYSWRLLFMTLHGKPRADHHVMEHVHESPGVMLVPLLVLSVGALAAGF
                     TLHSWFIGDNWPAFWGGSIFNGQHNHVLAQIEDVPFWVDITPTVVGLLGIALAYVMYI
                     ANPLLPVRLAQSFGGIYRFLLNKWYFDELYNVIFVQPAIRIARLLWQVGDATIIDGVP
                     NGLAELTSDGSKQVVKIQTGSLAVYAFVMLIGVVVLVGIFMLSR"
     gene            1317..>2096
                     /locus_tag="VGC82_18355"
     CDS             1317..>2096
                     /locus_tag="VGC82_18355"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY0425287.1"
                     /translation="MNVVSNAAGFPILSLITWLPLVGCLAMMMVRGDEETVASNARWT
                     ALWTSLLVLILSLVLWVRFDPSDGGFQFNERIAWMPEFKVAYSMGVDGISVLFVLLST
                     VLTPICILASWESIQVRVREFMMSFLILETMMVGMFAATDFVVFYMFFEGVLIPMYLI
                     IGVWGGPRRVYAAVKFFLFTLTGSVLMLLALVAMWLQAGTTDIAALMYTPFPPGMQNW
                     LFLAFLASFAVKVPMWPVHTWLPDAHVEAPTAGSVILAGVLL"
BASE COUNT          315 a          640 c          670 g          471 t
ORIGIN      
        1 cgcctcggac acctacgcgc tgttcggcac caatttcccg gccatcgagg tcatggcatt
       61 cctgctgttc atcggcgcga tgggcaaatc ggcgcaaatt ggcttccatg tctggctgcc
      121 ggacgcgatg gaggggccga cgccggtctc ggcgctgatc catgccgcga cgatggtgac
      181 cgccggggtg ttcctgatgg cgcgcatgtc gccgttgatg gagtatgcgc ccgtcgcgct
      241 ggatttcgtc accttcatcg gcgccacgac cgcgctgttc gccgcgacga tcggctgcgt
      301 gcagaacgac atcaagcgcg tcatcgccta ttccacatgc tcgcagctcg gttacatgtt
      361 cgtcgcggcc ggcgtgggcg tgtaccaggc ctcggtcttt cacctgatta cccatgcgtt
      421 cttcaaggcg ctgctgttcc tcagcgccgg ctccgtcatc catgccatgt ccgacgagca
      481 ggatatgcgg aagatgggcg ggatctggaa attcattccg gtgacctacg cggtgatgtg
      541 ggtcggcagc ctggccttgg ccggggtgtt cccgtttgcc ggatactatt cgaaggatgc
      601 gatcatcagc ggcgcctatg cggcgcattc cggcatcggc acctatggct tcatctgcac
      661 cgtgctggcg gcgttcctga ccgcgttcta ctcgtggcgc ctgctgttca tgaccttaca
      721 cggcaaaccc cgagcggacc atcatgtgat ggagcatgtg catgaaagcc cgggggtcat
      781 gctggtgccg ttgctggtgc tgtccgtcgg tgcgctggct gccggcttca ccctgcatag
      841 ctggttcatc ggcgacaact ggcccgcgtt ctggggcggc agcatcttca atggccagca
      901 caaccacgtt ctggcgcaga tcgaggacgt cccgttctgg gtggatatca ccccaacggt
      961 ggtcgggctg ctcggcatcg cgctggccta cgtgatgtat atcgccaatc cgctgctgcc
     1021 ggtccgcctg gcgcagagtt tcggcggcat ctaccggttc ctgctgaaca aatggtactt
     1081 cgacgagctc tacaacgtga tcttcgtgca gccggcgatc cgcatcgccc gcctgctgtg
     1141 gcaggttggc gacgcgacga tcatcgacgg agtgccgaac ggcctcgcgg agctgaccag
     1201 cgacggatcc aagcaagtgg tgaaaatcca gaccggctcg ctcgcggtct atgccttcgt
     1261 catgctgatc ggcgtcgtgg tgctggtcgg tatcttcatg ctgagccggt gatcctatga
     1321 acgtagtcag caacgccgcc ggctttccaa tcctgtcgct gatcacatgg ctgccgctgg
     1381 ttggttgcct ggcgatgatg atggtccgcg gcgacgagga gaccgtcgcg tcgaatgcca
     1441 gatggaccgc gctgtggacc agccttctgg tactgatcct gtcattggtg ctgtgggtga
     1501 ggttcgatcc gtccgacggc ggcttccagt tcaacgagcg catcgcctgg atgccggaat
     1561 tcaaagtcgc ctacagcatg ggcgtggacg ggatttccgt gcttttcgtg ctgttgtcga
     1621 cggtgctgac gccgatctgc atcctcgcga gctgggagtc gatccaggtc cgggtccgcg
     1681 agttcatgat gagcttcctg atcctggaaa ccatgatggt gggcatgttc gccgcgacgg
     1741 atttcgtggt gttctacatg ttcttcgaag gcgtgctgat cccgatgtac ctcatcatcg
     1801 gcgtctgggg cgggcccagg cgggtttatg ccgcggtcaa attcttcctg ttcacgctga
     1861 cgggttcggt gctgatgctg ttggcgctgg tcgcgatgtg gctgcaggcc ggcacaaccg
     1921 acatcgccgc gctgatgtac accccgtttc cgcccggcat gcaaaactgg ctgttcctgg
     1981 cgttcctggc cagcttcgcg gtcaaggtgc caatgtggcc ggtccacacc tggctgcccg
     2041 acgcgcacgt cgaggcgccg accgccggct cggtcatcct ggccggcgtg ctgctg
//
LOCUS       DASWQN010001073         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1951613, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001073 DASWQN010000000
VERSION     DASWQN010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951613"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VGC82_18360"
     CDS             complement(<1..399)
                     /locus_tag="VGC82_18360"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0425288.1"
                     /translation="MHPYEWEPPSASIARQAGVPEDQVVRFDTNTFPWPGVALQDLPP
                     LALNEYPDTSYGALTQAIADYVDVAPDRITVGAGADEILDLCAKAYIDASTPVVLARP
                     TYPMFRILTEIAGGPIEEVPMVDLRLDRAAF"
     gene            503..1414
                     /locus_tag="VGC82_18365"
     CDS             503..1414
                     /locus_tag="VGC82_18365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY0425289.1"
                     /translation="MDQAQTPALARRPDSKASAALYSVAVNLVLIAAKLTVGLLTGSL
                     AILADAAHSFLDLSASIFAYAGIRTAEKPADEEHAFGHAKAENISSLIQMFLLGATCI
                     AIVIESVRRLVVSSPVRVAWYSFIVVLGAVVIDIVVSRYLARVSKAHGGSAALDADAL
                     HFTSDIWASIAVFIGITLVAIFGFQWADPAAGIAVAIIIGSTAVRQGRETTQVLLDAM
                     PDRKTLAAIERILETDPQLHGYHALRARQAGKSVLLDVSVHVDGQLTLAKAHEVGHAV
                     SERIRRELPVVSDAVVHVEPVDDESHG"
BASE COUNT          256 a          490 c          500 g          288 t
ORIGIN      
        1 gaatgcggcg cggtcaagcc gcaggtccac catcggcacc tcctcgatcg gcccgcccgc
       61 gatctcggta aggatccgga acatcggata ggtcggacgc gccaggacga cgggagtgct
      121 ggcatcgatg taggccttgg cacacagatc gaggatttcg tcagcaccgg cgccgaccgt
      181 aatgcggtct ggcgcgacgt cgacataatc ggcaatcgcc tgggtcagtg ccccgtagct
      241 ggtgtcggga tattcgttca gtgccagggg cggcagatcc tggagcgcga ctccaggcca
      301 cggaaacgtg ttggtgtcga agcgcaccac ctggtcctcg ggaacgccag cctggcgggc
      361 gatgctggcg ctgggcggct cccattcata aggatgcatg cgctggatag ccggccgcat
      421 ctgtggaggc tggaatgatt tcggtcccat atctgtatct tggcggttgc aagtacccgc
      481 cggatgggta gttttacggg tgatggacca ggctcaaacg ccggcactcg cccggcggcc
      541 tgatagcaag gcttctgcgg cgctctactc ggtcgcggtc aacctggtcc tgatcgcggc
      601 caagcttacg gtcggactgt taaccggcag cctggccatc ctggccgacg ccgctcactc
      661 gtttctggat ctttcggcaa gcatctttgc ctacgccggg atccgcaccg ccgagaagcc
      721 ggctgacgag gaacacgcct ttggccacgc caaggcggag aacatctcga gcctgatcca
      781 gatgttcctg ctgggcgcca cctgcatcgc gatcgtgatc gagtcggtgc gccggctggt
      841 ggtcagctcg ccagtccggg tcgcctggta ctccttcatc gtcgtccttg gggccgtcgt
      901 gatcgacatc gtggtcagcc gctacctggc ccgcgtatcg aaagcccatg gcggcagtgc
      961 cgctctggat gccgacgccc tgcacttcac ctcggacatc tgggcgtcga tcgccgtctt
     1021 catcggtatc actctggtag cgatattcgg cttccagtgg gctgatcccg ccgccgggat
     1081 cgcggtggcg atcatcatcg gcagcaccgc cgtccgccag ggtcgcgaaa cgacccaggt
     1141 cctgctggac gccatgccag accgaaaaac gctggccgcg atcgagcgaa tcctggagac
     1201 cgatcctcag cttcacggct accacgcgct gcgcgcccgg caggcgggca agagcgtact
     1261 gctggatgtg tccgtgcacg tcgacggtca actcaccctg gcgaaggcgc acgaagtcgg
     1321 tcacgccgta tcggagcgaa tccggcgtga gctccccgtg gtgtccgacg ccgtggtgca
     1381 tgtcgaaccg gtcgacgacg agtcccacgg atgaagcgag actggcgggc agccgccatc
     1441 tgggacgcgg tgttgctcgg agtgccgctc gtgatcgcag gaatccttac cttcaatggc
     1501 tggccggcgg gcaatgcccg cattctgctg cttg
//
LOCUS       DASWQN010001074         4257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_368832, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001074 DASWQN010000000
VERSION     DASWQN010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368832"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VGC82_18370"
     CDS             complement(<1..384)
                     /locus_tag="VGC82_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425290.1"
                     /translation="MENGILKSVGVVPKPLQKPHPPVFLPFASSENSIRWAATEGVTA
                     ILPPMLPVYENHLYDVYAEVSGRPRGHGTGLLRDVIIADTDEEAMALWRDSGQFTGRA
                     WFEPFGFRRGLQDPKTGGYLTPEEVV"
     gene            complement(366..983)
                     /locus_tag="VGC82_18375"
     CDS             complement(366..983)
                     /locus_tag="VGC82_18375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0425291.1"
                     /translation="MKASLFYLPSIGSRAEIEAGMAGMRPELYQRMLAELSEQIRIAD
                     DLGYDSVSFTEHHFHIEGFELSNNPVLLDLYFAMQTKRIRVGQLGIVLPAANPIRVAE
                     DIAMLDHMTGGRANAGFARGYQRRWVDIMAQQTHGVHGALPHQHDEIDAANRAAFEEN
                     FQIIKKAWTQEMLTHDGKYGKYRPAKRPGPWIPRPNTARAWRTAS"
     gene            1093..2226
                     /locus_tag="VGC82_18380"
     CDS             1093..2226
                     /locus_tag="VGC82_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0425292.1"
                     /translation="MLKRSTQSSLRYSLLGAGAAFAIVLGGAAMPARAQIHIATAGPM
                     TGEYAAFGDQMKKGAEQAVADLNKAGGVLGQQLVLDIGDDACDPKQAVSVANQLASKK
                     VKLVAGHYCSGSSIPASKVYSEEGILQISPASTNPKYTDEGSWNTFRVCGRDDEQGKV
                     AGDYLAKHFKDEKIAILNDNSAYGKGLADETAKALHAAGGKEVLATAYTPGEKDYSSL
                     VSRLKQAGVSVIYVGGYHTEAGLIIRQAKEQGLKATMVGGDALVTTEFWQIAGESGAG
                     TMMTFPPDPRLMPTAAEVVKAFKAKKIDPEGYILYTYAAIQIWADAAKKAGTTDPKKV
                     AAALKASGNWPSVLGPVSFDKKGDTTGGGYVFYLWKDGSYTQM"
     gene            complement(2338..3384)
                     /locus_tag="VGC82_18385"
     CDS             complement(2338..3384)
                     /locus_tag="VGC82_18385"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0425293.1"
                     /translation="MAKSCTCCGVGVLSRRGLLTGATALGAAGLFRTADAADKAFRID
                     VHHHISPPSWLEAVKKAKLDNPPMVNWSPERSLEDMDKAGVATAMTSPTTPQLGFLPA
                     AEAARIAREANEYARKVANDYQGRFGMFAMLPMPYVDESLKEIAYACDTLHADGIGMM
                     TSYGDKWLGYSEFQPVFDELNRRKAVVYTHPTGPNCCVNLVRGVPEAAVEYGTDTTRT
                     IVNLIFSGASQRYADINFIFSHGGGVLTAVAERLQIQMVKTPPYAGKFTRDQVQHELN
                     RFFYDTAQVSNAVTIEALAQLVPPSQIVFGSDFPYRTAEEHVTGLAQHFKGEVLRGIE
                     RDNAARILPGVRPV"
     gene            complement(3418..3921)
                     /locus_tag="VGC82_18390"
     CDS             complement(3418..3921)
                     /locus_tag="VGC82_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEY0425294.1"
                     /translation="MRVVPVEKQAYREAMARLGAAVNVITTDGPGGRAGFTASAVTSV
                     TDSPPTLLVCANRTNDSYPAMRQNGVLCVNTLTPAQEGLSPVFAGMTQHKMNERFDGY
                     AWHTLETGAPVLDQAVVVFDCRIEQVVEVGTHDVFFCSVEAVRTGKAHEGLIYYGRGY
                     HKVAIID"
BASE COUNT          776 a         1360 c         1322 g          799 t
ORIGIN      
        1 caccacttcc tcgggcgtca ggtagccgcc ggtcttcggg tcctgcaggc cgcggcggaa
       61 gccgaacggc tcgaaccagg cgcggccggt gaactggccg gagtcgcgcc acagcgccat
      121 cgcctcctca tccgtgtcgg cgatgatcac gtcccgcagc aatcccgtcc catgacccct
      181 gggccgcccg gagacctcgg catagacgtc gtaaagatgg ttttcgtaca ccggcagcat
      241 tggcggcagg atcgccgtca cgccctcggt cgctgcccac cggatggagt tctcggacga
      301 cgcgaacggc aggaacaccg gcggatgcgg tttctgcagc ggcttcggga ccaccccgac
      361 cgacttcagg atgccgttct ccacgccctt gccgtatttg gccgtggtat ccagggtcca
      421 gggcgtttcg ccggccggta ctttccgtac ttgccgtcgt gcgtcagcat ttcctgcgtc
      481 caggccttct tgatgatctg aaaattctcc tcgaaggccg ctcggtttgc cgcgtcgatc
      541 tcatcgtgct gatgcggcag ggcgccgtgg acgccatggg tctgttgcgc cataatatcg
      601 acccagcgcc gctgatagcc ccgggcgaaa ccggcattgg cccgcccgcc ggtcatgtgg
      661 tccagcatcg cgatatcctc ggcgacgcgg atcgggttgg cggccggcag cacgatgccc
      721 aactgtccga cgcggatgcg cttggtctgc atcgcgaaat acaggtccag cagcaccggg
      781 ttgttggaaa gctcgaaccc ctcgatatgg aaatgatgtt cggtgaagct gaccgagtca
      841 tatcccaggt cgtctgctat ccggatctgt tcggacagtt ccgccagcat ccgctggtaa
      901 agctctgggc gcatgcccgc cattccggcc tcgatttccg cgcggctgcc gatgctgggc
      961 agatagaaca gggacgcctt cacgttttga tctccttgga agcttttatt ggtggatttg
     1021 ttgctggatt ctggcgcttt ttctcggccg ccgctaggct tccggcggac ttataaaaaa
     1081 cggggatgct cgatgctgaa aagatccacg caatcaagcc tgcgatacag cttgctcgga
     1141 gccggggcgg ctttcgccat tgtcctgggc ggcgcggcaa tgccggcccg ggcgcagatc
     1201 catatcgcga ccgccggacc gatgacgggc gagtatgccg cgttcggcga ccagatgaag
     1261 aaaggcgcgg agcaagcggt tgccgacctg aacaaggctg gcggcgtgct gggtcagcag
     1321 cttgtgctgg atatcggcga cgacgcctgc gacccgaagc aggcggtatc ggtggccaac
     1381 cagcttgcgt ccaagaaggt caagctggtg gccggtcatt actgctccgg cagttcgatt
     1441 cccgcgagca aggtttattc cgaggaaggc atcctgcaga tttccccggc gtccaccaat
     1501 cccaaataca ccgatgaggg aagctggaac accttccgcg tgtgcggccg tgacgacgag
     1561 cagggcaagg tcgccggcga ctatctggcc aagcacttca aggacgagaa gatcgcgatc
     1621 ctgaacgaca actcggccta cggcaaaggc ctggcggacg agacggcgaa ggcactgcac
     1681 gccgccggtg gcaaggaagt tctggcgacc gcctatacgc cgggcgaaaa ggattattcc
     1741 tcgctggtgt cgcgcctgaa gcaggctggt gtctcggtga tctatgtcgg gggctatcat
     1801 accgaggcgg ggctgatcat ccgtcaggcg aaagagcagg gcctgaaggc caccatggtt
     1861 ggcggcgatg ccctggtgac caccgagttc tggcagattg ccggggaatc aggcgccggc
     1921 acgatgatga cgtttccgcc tgacccgcgc ctgatgccga ccgcggctga ggtcgtgaaa
     1981 gcgttcaagg ccaagaagat cgatcccgag ggctacatcc tctacaccta cgcggcgatc
     2041 cagatctggg ccgacgcggc caagaaggct gggaccacgg atccaaaaaa agtcgcggcg
     2101 gcgctgaagg catccggcaa ctggccgtcg gttctaggcc cggtgagctt cgataaaaag
     2161 ggcgacacaa cgggcggcgg ctatgtcttc tacctttgga aggatggcag ctacacccag
     2221 atgtaggccg ttgggcggct gatcccgaac ccacgacttt cccgcattgg catagtcgtg
     2281 ggtggccacg tcaggcacgg ctatgatgca actccgccgg acaagcgggc ctaccgatca
     2341 gaccggccgg acacccggca gaatccgcgc ggcgttgtcc cgctcgattc cccgcagcac
     2401 ctcgcccttg aaatgctggg ccaatccggt gacgtgttcc tcggccgtcc gatacgggaa
     2461 gtccgagccg aacacgatct gggacggcgg caccagttgc gccagcgcct cgatcgtgac
     2521 cgcgttcgac acctgggcgg tgtcatagaa gaaccggttc aactcgtgct gcacctggtc
     2581 gcgggtgaac ttcccagcgt aaggcggcgt tttcaccatc tggatctgca agcgttcggc
     2641 caccgccgtc agcaccccgc cgccgtggga gaagatgaag ttgatatcgg catagcgctg
     2701 cgaggcaccg gagaagatca ggttcacgat cgtccgtgtc gtgtccgtgc cgtattccac
     2761 agcggcctcc ggcacgcctc gcaccaggtt gacgcagcaa ttcggccctg tcggatgcgt
     2821 gtacaccacc gccttgcggc ggttcagctc gtcgaacacc ggctggaatt cgctgtagcc
     2881 gagccatttg tcgccatagc tggtcatcat gccgatgccg tcggcgtgca gcgtgtcgca
     2941 cgcataggcg atttccttca ggctttcatc gacgtaaggc atcggcagca tggcgaacat
     3001 accgaaccgg ccttggtagt cgttggcgac cttgcgggcg tactcgttgg cctctcgggc
     3061 gatccgtgcc gcctcggctg ccggcaggaa tcccaactga ggcgtggtcg ggctggtcat
     3121 ggcggtggcg acgccggctt tatccatgtc ttccagcgat cgctccggcg accagtttac
     3181 catcggagga ttgtccagct tcgccttctt caccgcctcc agccaggacg gcggcgagat
     3241 atgatggtgc acatcgatcc ggaacgcctt gtcggcggcg tcggcggttc ggaacaatcc
     3301 cgccgcaccc agcgccgttg cgcccgtcag caggccgcgc cggcttaaga cgccgacgcc
     3361 gcagcacgta cagcttttcg ccatactcgt ttcccccgga atgaaccaac ccgccgatca
     3421 gtcgatgatc gcgaccttat ggtagccgcg gccgtagtaa atcaggcctt catgcgcttt
     3481 tccggtgcgt acggcttcca ccgaacagaa gaacacgtcg tgggtgccga cttcgacaac
     3541 ctgttcgata cggcaatcga acaccacgac ggcttggtcc agcaccggcg cgccggtttc
     3601 cagcgtgtgc caggcgtaac cgtcgaaccg ttcgttcatc ttatgctgcg tcatgccggc
     3661 aaagaccggc gacaggccct cctgcgccgg ggtgagcgtg ttgacgcaca gcaccccgtt
     3721 ctggcgcatc gccggatagg aatcgttggt gcgattggca cacaccaaaa gcgtcggcgg
     3781 cgagtcggtt acgctggtga ccgcactcgc ggtgaagccg gcgcgaccac caggcccatc
     3841 cgtggtgatc acattcaccg cagcacccag acgggccatc gcctcacggt aggcttgctt
     3901 ctccacgggc acgacacgca tcctccgctg tccgacgacg actatgcagc aggtcaacgc
     3961 cggaggccag ccgaccgccc ggcacggtca aaacggcccc acgccgttgc cttgttcctt
     4021 ctgcctgatt tcgcaacgcc gtgcctgcgg tcccggctga tcccgttcga ggaacattca
     4081 cgcgcttttc cgagttgttg caccacgatc ccgcgcattc gggtgcgcgt cggattatgc
     4141 tggagatcga cccggccccc ctggcaatgt cagcgaaacg atttgagatg agatcatgag
     4201 accccggttc atggatacaa cgcgccttcc cgatccgacc atgcgagccc agggctg
//
LOCUS       DASWQN010001075         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_632157, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001075 DASWQN010000000
VERSION     DASWQN010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632157"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VGC82_18395"
     CDS             <1..644
                     /locus_tag="VGC82_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY0425295.1"
                     /translation="SPLHWLGERLFCFHMIEHEIVMAVSAPLLVIARPIGTLLWSVPR
                     RARVTIGRFLKRPAVSRPWEVLSAGGIATLIHGIAFWAWHAPMLFDAAVTNVALHRLQ
                     HLSFLLTAILFWWSVLRRSNSGLAAWHLFVTMLHTSVLGALMALAPRVLYKAQTATAA
                     EWGLTPLEDQQLAGIIMWVPAGTVYAGAALALFLIWIRGASSTARSGNAIRAF"
     gene            625..1035
                     /locus_tag="VGC82_18400"
     CDS             625..1035
                     /locus_tag="VGC82_18400"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0425296.1"
                     /translation="MRFERFRHRWTLLCIVTFVVAVATAAALNWRAQHRSETLARAMV
                     GGDLARAPGIIRRYGCAGCHTIPGIPGGDGQVGGPLADIGRRVYVGGVISNTPDNLVQ
                     WIVSPQSFSPQSAMPATGITEAEARDVATFLYSR"
     gene            complement(1040..>1613)
                     /locus_tag="VGC82_18405"
     CDS             complement(1040..>1613)
                     /locus_tag="VGC82_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0425297.1"
                     /translation="PAGAHYYPAFPYTSYAKMKIADIKDLMAYLRTLPAVSGRPPPHD
                     LSVIFRVRPIVGLWKILFFDRTPIVAEPGRDETWNRGRYLAEAVAHCAECHSSRNLMG
                     AIKPDTRFAGGTDPEGTGYIPNITPSRIGDWNERDIAEMFKTGNTPNHGRVGSSMAAV
                     VTNLAMLPQSDRDAIAAFVKSLPSRETPRP"
BASE COUNT          266 a          463 c          537 g          347 t
ORIGIN      
        1 tatcgccgct gcattggctc ggcgagcgcc tgttctgctt tcatatgatc gaacatgaga
       61 tagtgatggc cgtttccgca cccctgctgg tgatcgcgcg gccgatcgga acgttgcttt
      121 ggtcggtccc gcgtcgggcc cgggtgacca ttggccggtt cctgaagcga cccgccgtta
      181 gtcgaccgtg ggaagtgctg agcgcgggag gtattgccac gcttatccac ggtatagcgt
      241 tctgggcctg gcatgctccg atgttgttcg acgcggccgt tacgaacgtt gcgctccatc
      301 gactgcagca cctcagcttc ctgctgacgg ccattctgtt ctggtggtcc gtgctccgac
      361 gcagtaattc cggattggca gcctggcatc tgttcgtcac gatgctgcac acaagcgttc
      421 tcggcgcgct tatggccctt gcgccgcggg tgctgtacaa ggcgcagacg gcaacggcag
      481 cagagtgggg gctgacccct ctggaggatc agcagctggc ggggatcatc atgtgggttc
      541 ccgccggaac agtctatgcc ggggcggcgc tggcgttgtt tctgatctgg atccgcggag
      601 cgagttcaac ggcgaggtca ggtaatgcga ttcgagcgtt ttagacaccg ctggacactc
      661 ctctgcatcg taacgtttgt cgttgctgtt gccaccgctg ccgcgttgaa ctggagggcg
      721 cagcaccggt ctgaaaccct ggctcgtgcc atggtgggtg gggacctcgc ccgcgcgccg
      781 ggcatcatca ggcgctatgg atgtgccggg tgtcatacga tccccggcat tcccggcggg
      841 gatggccaag tcggcggccc attggctgat atcgggcggc gcgtctacgt cgggggtgtc
      901 atcagcaata cgccggacaa cctcgttcag tggattgtgt cgccgcaatc attctcaccg
      961 caatcggcca tgccggcaac cgggatcacc gaggcggagg cgcgggacgt cgcgaccttt
     1021 ctttattcgc ggtgagcacc taagggcgcg gcgtctctcg ggaaggcagc gatttgacaa
     1081 aagccgcgat ggcgtcgcgg tcgctttgcg gcaacatggc caggttggtg acaacggcgg
     1141 ccatggaaga cccaacacgg ccgtggttgg gggtgttacc ggttttgaac atttccgcga
     1201 tgtcgcgttc gttccagtcc ccgatgcggg atggggtgat gttgggaatg tacccggtgc
     1261 cttccgggtc ggttccccct gcaaagcggg tgtcaggctt gatagccccc atcaggtttc
     1321 gagaggagtg acattcggcg caatgcgcca ccgcctcggc aagataccgg cctcggttcc
     1381 aggtctcgtc gcggccgggc tcggcaacaa ttggtgtccg atcgaagaac agaatcttcc
     1441 ataggccaac gatcggcctg acgcggaaga tcacgctcaa atcgtgaggc ggtggcctgc
     1501 cggagactgc gggcagggtc cggagatacg ccatcaggtc cttgatgtcg gcgatcttca
     1561 tttttgcgta actcgtatag ggaaatgctg gatagtagtg ggctcccgcc gga
//
LOCUS       DASWQN010001076         4162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2084193, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001076 DASWQN010000000
VERSION     DASWQN010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084193"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2093)
                     /locus_tag="VGC82_18410"
     CDS             complement(<1..2093)
                     /locus_tag="VGC82_18410"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit H"
                     /protein_id="HEY0425298.1"
                     /translation="MMRKPTTAANRTPVRVVVVTMDSHLGGALSRAEGELRRELPNLE
                     VTMHAADEWGGNPAALAACHADIGRADIVVAAMLFLEDHIRAVLPALTARREHCDAMI
                     CMLSAGEIVRLTRAGKFNMSAEATGAVAWLKRMRGKSGTGSNARGQMKMLKQLPKLLR
                     FIPGTAQDVRAYFMTLQYWLAGSQENLGSMVRLLVGRYAAGDRAHLAGSLLVPPPVEY
                     PEVGLYHPRAASRITEHLDQIPAGGKNGKVGVLLLRSYALSSNAAHYDGVIAALEAKG
                     LQVIPAFASGLDQREALTRYFISNGRATVDAVVSLTGFSLVGGPAYNDARAAEALLSS
                     LDVPYIAAHPVEFQTLEQWQDDARGLLPVEATMMVAIPELDGAICPMTFGGRSTTTGD
                     ARRRDMVAHPERAAMLAARVARLVRLRRKARADRKIAVVLFNFPPNAGTVGSAAYLSV
                     FASLLNTLRGMKDAGYDVAVPESVDALRDRLLGGNSKLFGANANVAARVPVDEHVRRQ
                     RWLNEIEAQWGPAPGRLQTDGASLFVLGEHFGNVFVGVQPAFGYEGDPMRLLFEHGFA
                     PTHAFCAFYRWINQEYGADAVLHFGTHGALEFMPGKQAGMSGECWPDRLIGDLPNFYL
                     YASNNPSEGMIAKRRAGASLISYLTPPVAHAGLYRGLLDLKASLDRWRALPPDADTGQ
                     RDDLAAMIQAQAEAV"
     gene            complement(2065..3555)
                     /gene="bchB"
                     /locus_tag="VGC82_18415"
     CDS             complement(2065..3555)
                     /gene="bchB"
                     /locus_tag="VGC82_18415"
                     /EC_number="1.3.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin:protochlorophyllide reductase
                     (ATP-dependent) subunit B"
                     /protein_id="HEY0425299.1"
                     /translation="MKLTLWTYEGPPHVGAMRVATAMEGLHYVLHAPQGDTYADLLFT
                     MIERQSKRPPVTYTTFQARDLGGDTAELFKNAARQAYERFKPQALLVGASCTAELIQD
                     DPGGLAKALGLPIPVVAVDLPAYQKKENWGSAETFYQLVRAFAVRGSPSEQARCNILG
                     PTALGFRHRDDVAEVVRLLDRLGVAVNLVAPLGATPADLVRLGDASFNIVLYPETGMQ
                     AALWLSRQFGQPMVKTVPIGVGATRDFIAEVAALAGVEPLDDERSRLPWYSRSVDSTY
                     LTGKRVFIFGDATHAIAAARIAASELGFTVVGLGSYSREFAREVRQAATLHGVEALIT
                     DDYLEVEAHITELQPELVLGTQMERHIAKRLGIPCTVISAPVHVQDFPARYSPQMGFE
                     GANVIFDTWVHPLMMGLEEHLLGMFRDDPEFHDEAGASHRPESASPAAPVLSLSWDNA
                     AEKELRKIPFFVRGKARRNTERFAADRGLKDITLETLYDAKAHYGR"
     gene            complement(3560..>4162)
                     /locus_tag="VGC82_18420"
     CDS             complement(3560..>4162)
                     /locus_tag="VGC82_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HEY0425300.1"
                     /translation="AQPFLADTARALEERGATRIPAPFPLGVEGTTLWLQAAASAFGV
                     GAFTFQAAIELARERGHRAVARYRNELAGKRMFLFPDSQLEIPLARFVSRELSMQVIE
                     AGTPFLHRQHLAEELALLPESVVLSEGQDVERQLDRCRTARPDLVVCGLGLANPLEAF
                     GLTTKWSIELLFTPVQGYDQAGDLAELFARPLLRRMRLAV"
BASE COUNT          796 a         1416 c         1242 g          708 t
ORIGIN      
        1 tcgaccgcct cggcctgcgc ctggatcatc gcggcaaggt catcgcgttg cccggtatcc
       61 gcgtccggcg gcaacgcccg ccagcggtcg agcgacgcct tcaggtccag cagtccgcga
      121 tacagcccgg catgcgcgac gggcggcgtc aggtagctga tcagcgaggc ccccgcacgg
      181 cgtttggcga tcatgccttc ggacggattg ttggaggcat aaagatagaa gttcggcagg
      241 tcgccgatca gccggtcggg ccagcactcg cccgacatcc cagcctgctt gccgggcatg
      301 aattccaacg caccatgggt gccgaagtgc aacaccgcgt ccgcgccgta ttcctggttg
      361 atccaacggt agaaagcgca gaacgcgtgg gttggcgcaa agccgtgttc gaacagcagc
      421 cgcatcggat cgccttcgta tccgaatgcc ggctgcacgc cgacgaagac attgccgaaa
      481 tgctccccca gaacgaacaa agatgcgcca tcggtctgca aacggcccgg cgcaggaccc
      541 cattgcgctt cgatctcatt caaccagcgc tgccgccgga catgctcatc gacgggcacc
      601 ctggcagcga cgttggcgtt ggcgccaaac agtttcgaat tgccgcccag cagccggtcg
      661 cgcagcgcat cgacgctttc aggcaccgca acgtcgtatc cggcgtcctt catgccgcga
      721 agcgtgttca gcaatgaggc gaaaactgac aggtaggccg ccgatccgac cgtcccggcg
      781 ttcggcggga agttgaacag cacgaccgca atcttccggt ccgcacgcgc cttacggcgc
      841 aatcgcacaa gacgcgcgac cctggccgcc agcatcgccg cgcgttccgg atgcgcgacc
      901 atatcgcgcc gccgcgcatc gcccgtggtg gtggatcggc cgccgaacgt catgggacag
      961 atcgcaccgt ccagttccgg gatcgccacc atcatcgtgg cctcgaccgg cagcaggccg
     1021 cgcgcgtcgt cctgccactg ttccagtgtc tggaattcca ccggatgggc ggcgatatag
     1081 ggcacgtcca gactgctcag cagcgcttcg gcggcacggg catcgttgta ggcaggaccg
     1141 ccaaccaggc taaatccggt caaggaaacc acggcgtcaa cggttgcgcg gccattgctg
     1201 ataaaataac gagtcagggc ctcacgctga tccagcccgc tggcgaatgc cggtatcacc
     1261 tgcagccctt tcgcctccag cgccgcgatc acgccatcat agtgcgccgc gttgctcgac
     1321 agggcgtagg atcgcagcag caaaacgccg accttgccat tttttccgcc ggccggaatt
     1381 tgatccagat gttcggtgat ccggctggct gcacgagggt gatacaatcc gacctcggga
     1441 tattccaccg gaggcggcac cagcagcgat cccgccagat gcgcccgatc accggccgcg
     1501 taacgtccaa ccagcagccg caccatgctg cccaggttct cctgcgaccc ggccagccaa
     1561 tattgcagcg tcatgaagta agcgcggaca tcctgcgcgg tgcccggaat gaaccgcagc
     1621 agcttcggaa gctgcttcag catcttcatt tggccacggg cattcgatcc ggtgccgctc
     1681 ttgccgcgca tccgcttcag ccaggcaacc gcacctgtcg cctcggctga catgttgaac
     1741 tttccggcgc gcgtcagccg cacgatttca cccgccgaca gcatgcagat catggcatcg
     1801 cagtgttccc gccgcgccgt caacgcgggc agcacagcgc gaatatggtc ctccaggaac
     1861 agcatcgccg cgaccacgat atcggcgcgc ccgatgtcgg catgacaggc agccaacgcc
     1921 gcgggattgc cgccccactc atcggccgca tgcatcgtca cttccaggtt cggcagttcc
     1981 cggcgcaatt caccttccgc acgcgacagt gctccgccga ggtgactgtc catcgtcacc
     2041 acgacaaccc gcaccggagt acggttagcg gccgtagtgg gctttcgcat catataaggt
     2101 ttccagggtg atatctttca ggccacgatc ggcagcgaaa cgttccgtgt tgcggcgcgc
     2161 tttgccgcgg acgaagaacg ggattttcct caattccttc tcggccgcat tgtcccaact
     2221 tagactcagg accggcgcgg cgggcgatgc ggactctggc cggtgcgacg caccggcttc
     2281 atcgtgaaat tcgggatcat cgcggaacat accaagcaga tgctcctcga ggcccatcat
     2341 gagcggatgc acccatgtgt cgaagatcac gttggcgcct tcgaagccca tttgcgggga
     2401 gtatcgtgcc gggaaatcct gcacatgcac cggtgcggat atgaccgtgc acggaatgcc
     2461 caaccgcttg gcgatgtgcc gctccatctg cgtcccaaga acgagttccg gctgcagctc
     2521 ggttatgtgt gcctcgacct ccaggtagtc gtcggtgatc agtgcctcga caccatgaag
     2581 cgttgcggcc tggcgaacct cgcgggcaaa ttcgcggctg tagctgccga gcccgacgac
     2641 ggtgaagcca agctcgcttg cggcgatgcg tgccgcggcg atcgcatggg tcgcatcgcc
     2701 gaagatgaag acccgcttgc cggtcagata agtggaatcg accgacctgg agtaccaagg
     2761 cagcctcgac cgttcgtcgt ccaaaggctc gacgccggcc agtgccgcga cttcggcaat
     2821 gaaatcccgg gtggccccga caccgatcgg cacggtcttt accatcggct gaccgaactg
     2881 gcgcgacagc cacaacgccg cctgcatacc ggtttcagga tacaggacga tgttgaacga
     2941 agcatcgccc agccgaacca gatcggctgg cgtagcgcct aacggagcca ccagattgac
     3001 cgccacgccg aggcgatcca gcaaccgaac gacctcggcg acatcgtccc gatgccggaa
     3061 ccccagcgcc gtcgggccca gaatgttgca gcgcgcctgc tcgctcggag acccccgcac
     3121 cgcgaaagcg cggacgagct gatagaaagt ttccgccgat ccccagtttt cctttttctg
     3181 gtaggccggc agatcgaccg cgaccactgg gatcggcaat cccagggcct tggccaagcc
     3241 gcccggatcg tcctgaatca gctccgccgt acaggacgcg ccgaccagca atgcctgcgg
     3301 cttgaatcgc tcataagcct gccgtgccgc gttcttgaac agctccgcgg tgtcgccgcc
     3361 gaggtcgcga gcctggaacg tcgtgtaggt caccggcggc cgtttcgact gccgttcgat
     3421 catggtgaac aacaggtcgg cataggtgtc gccctgcggc gcgtgcagca cgtagtgcag
     3481 cccctccatc gccgtcgcca cgcgcatcgc gccgacatgg ggaggacctt catatgtcca
     3541 gagcgtcagc ttcatcgcgt tataccgcca accgcatgcg ccgaagcagc ggacgcgcga
     3601 acagctccgc gaggtcgccg gcctggtcgt agccttgcac cggcgtgaac agcagctcga
     3661 tcgaccattt tgtggtcagg ccaaaagcct ccagcggatt tgccagtccc agaccgcaaa
     3721 ccaccaggtc gggccgcgcc gttcggcatc tgtccagttg acgctcgaca tcctgtcctt
     3781 cgctcagcac gacagactcc ggcagtaacg cgagctcttc cgcgaggtgt tggcgatgca
     3841 gaaacggtgt ccccgcctcg attacctgca tcgacagctc tcgcgaaaca aaccgagcca
     3901 ggggaatttc cagctgcgag tcgggaaaca ggaacatccg ctttccggcc agttcgttgc
     3961 gatagcgggc caccgcacgg tggccgcgct cgcgcgccaa ctcgatcgcc gcctggaacg
     4021 taaatgcgcc tacaccgaac gccgaagccg cggcctgcag ccaaagcgtg gttccctcga
     4081 caccaagcgg gaatggtgcc gggatccgcg ttgcgccgcg ctcttccaac gctcgcgccg
     4141 tatccgccag gaagggctga gc
//
LOCUS       DASWQN010001077         2402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_104726, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001077 DASWQN010000000
VERSION     DASWQN010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104726"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="VGC82_18425"
     CDS             <1..1635
                     /locus_tag="VGC82_18425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEY0425301.1"
                     /translation="VSRGYRKSEGYMFHQLLTPVAGSLPLSFLVAVSPIAVVLVMLGV
                     LRRPAWQASLGGLVVAFVVAVTAWQMPVGLALDSAAAGAVFALWPVMWIVFNALLLYN
                     IAVASGRFDAFRDWVLDHLPNDRRIVLVVIGFCFGCLLEGISGFGTPVAITSALLILV
                     GFPPLEALTFTLIFNTAPVAFGALGAPVTVLGQVTGLPAVQLGAMIGRQLPFLAMLLP
                     FYVMAIYGGARSVKALWPVLLVSGVSFAAGQFISSNYLDYALTDVLSSLTALIVTLGF
                     LRVWKPAPDAAFAVRAKVAAVRPTDAVPAWQGWLPWIVVSAVVIVWTHLKVFAIGQQA
                     IAWPGLHNQIAITLYNDKPYAAVWVFQPLATGTAILLAAIITAAMVGVGPGRFFTAVG
                     ATWKQSRIAILTVALIVALAYLMNYSGMNYTLGKGVASMGFIFPLVSPFLGWVAVFLS
                     GSDTSGNALFGNLQVVAANQLGLNPVLIAATNSSGGVMGKMISPQNIATGVSVTDLKG
                     QEGVVFARTFKHSIALTLLLGVLVVLQQYVVPWMIP"
     gene            1632..>2402
                     /locus_tag="VGC82_18430"
     CDS             1632..>2402
                     /locus_tag="VGC82_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425302.1"
                     /translation="MRIAQEVPIAATGERPQSGGNVFESKPRTMIFLMKNRAAVVALG
                     ALLAFGAGARAEVPEVRIARQFSMGYLQFNVIEHEKLIQKHAAALGIPEVKVSGVRFN
                     GPASMNDALISDSVDIVGGSPNGMFTLWAKARGTPQEVRAITALVTLPYAITSNDPEI
                     KTIADLGKCKKIPVPSVKVSSPAVTIQQAAAKLYGIKEFARFDAATVTMSPADATIAL
                     LTKSADVNCVVALPPYMQQQLEHPEIHVVGNSFDLAGGP"
BASE COUNT          365 a          722 c          813 g          502 t
ORIGIN      
        1 gtatctcgcg gataccgaaa gtctgaggga tacatgttcc atcaattgct gacgccggtt
       61 gccggcagtc tgccgctctc gtttctggtt gccgtgtcgc cgatcgccgt cgtgctggtc
      121 atgctggggg tgctgcgccg gcccgcatgg caggcttcgc tgggcggact ggtcgtcgca
      181 ttcgttgtcg ccgtgaccgc ctggcagatg ccggtcgggc tggcgctcga cagcgcggcc
      241 gcgggcgcgg tgtttgcgct gtggccggtc atgtggatcg tgttcaacgc gttgctgttg
      301 tacaacatcg cggtcgcctc cggccgtttc gatgcgtttc gcgactgggt gctggaccat
      361 ttgccgaatg accggcggat cgtgctggtg gttatcgggt tctgcttcgg ctgcttgctg
      421 gaaggtatct cgggctttgg gacgccggtg gcgatcacca gcgcgctgct gatcctggtc
      481 gggttcccgc cgctggaagc gttgacgttc accctgatct tcaataccgc acccgtggcg
      541 ttcggcgcct tgggggcgcc ggtgaccgtg cttggccagg tcaccggcct gcccgccgtg
      601 caacttggcg ccatgatcgg acggcaattg ccgttcctgg cgatgctgct gccgttctac
      661 gtcatggcga tctatggtgg agcgcgttcg gtcaaggccc tgtggccggt gcttctggtt
      721 tccggcgtca gcttcgccgc ggggcagttc atatcgtcga actacctgga ctacgcactg
      781 accgacgtgc tgtcgtcgct gacggcactg atcgtgaccc tcggtttcct gcgggtctgg
      841 aagcccgcgc ccgatgccgc tttcgccgtc cgcgcgaagg ttgccgcggt tcggccgacg
      901 gatgccgttc ccgcctggca aggctggctt ccctggatcg tggtgtccgc cgtcgtgatc
      961 gtttggactc acctgaaggt ctttgccatc ggccagcagg cgattgcctg gcccgggctg
     1021 cacaaccaga tcgcgatcac gctgtataac gacaaaccct acgccgctgt ctgggtgttc
     1081 cagccactgg cgacggggac ggcgatcctg ctggcggcga tcatcaccgc ggcgatggtc
     1141 ggagtcggtc cgggacggtt tttcaccgcc gtcggggcga cgtggaagca aagccggatc
     1201 gccatcctga cggtggcact gatcgtggcg ttggcgtacc tgatgaacta ctccggcatg
     1261 aactacacgc tcggcaaggg cgtcgcctcg atgggcttca tcttcccgct ggtttcaccg
     1321 ttcctgggct gggtcgcggt gttcctgtcc ggcagcgaca cttccggcaa cgcgctgttc
     1381 ggcaatcttc aggtcgtggc ggcaaaccag ctcgggctga atccggtgct gatcgcagca
     1441 acgaattcct cgggcggggt gatgggcaag atgatctcgc cgcagaacat cgcaacgggc
     1501 gtgtcggtga cggacctgaa ggggcaggag ggcgtggtgt tcgcgcgcac cttcaagcac
     1561 agcatcgcgc tgaccctgct gcttggtgtg ctggtggtgc ttcagcagta tgtcgtgccg
     1621 tggatgatcc catgaggatc gctcaggagg tgccaatcgc cgccaccggc gagcggcctc
     1681 aatctggtgg aaacgtcttt gaaagcaagc cgcggaccat gatcttcctg atgaaaaacc
     1741 gagcagcggt cgtggcgctg ggcgcattgc tggcgtttgg cgcgggggcg cgggccgaag
     1801 tgccggaggt ccgcatcgcc aggcaattct ccatgggtta cctgcagttc aacgtgatcg
     1861 agcatgagaa gctgatccag aaacatgccg cggcgttggg cattcccgaa gtgaaggttt
     1921 ccggcgttcg cttcaatgga cccgcgtcga tgaacgatgc gctgatctcg gactcggtcg
     1981 atatcgtcgg cggcagcccc aacggcatgt tcaccctgtg ggccaaggcg cgcggcacgc
     2041 cgcaggaggt gcgggcgatc accgcgctcg tgacgctgcc ctatgcgatc accagcaacg
     2101 atcccgagat caagacgatc gccgaccttg gcaagtgcaa gaagatcccg gtgccttcgg
     2161 tgaaggtttc ctcgccggcg gtgacgatcc agcaggcggc ggcgaagcta tacgggatca
     2221 aggagtttgc tcggtttgac gctgcgacgg tgacgatgtc gccggccgac gcaaccatcg
     2281 cactgctgac caaaagcgcg gacgtgaact gcgtcgtcgc gctgccgccg tatatgcagc
     2341 agcagttgga acatccggag atccatgtgg tgggcaactc gttcgacctg gccggcggtc
     2401 cg
//
LOCUS       DASWQN010001078         7336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1424696, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001078 DASWQN010000000
VERSION     DASWQN010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424696"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VGC82_18435"
     CDS             complement(<1..585)
                     /locus_tag="VGC82_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY0425303.1"
                     /translation="MSGSLAALPWPIWGIAAAATAGVIFRPFSWPEFIWSVAGAVLLL
                     ALGLLAPADAWAGVAKGSDVYLFLIGMMLLAELARQEGLFDWLAAKAAGMARGSPTRL
                     FSLVFAVGTIVTVFLSNDATAVVLTPAVAAVVKAAKAKEALPYLLVCAFIANAASFVL
                     PISNPANLVIYGSHMPPLLQWLRLFAAPSALSILA"
     gene            complement(582..1844)
                     /locus_tag="VGC82_18440"
     CDS             complement(582..1844)
                     /locus_tag="VGC82_18440"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425304.1"
                     /translation="MNTSSQHPSSRSWRGLDGINFLMADVRDGVGPYLSIYLKGAQHW
                     DSGAIGLAMGASSIAAAICQIPAGLLVDGSRKKRLLIAGSGLMVGLGCLLIVLFPRFW
                     TVIAAQAMLGAASAVIPPAIAAVSLGLVGRRLLDGRVSRNESFNHAGNLTAAVLAGTL
                     GQYLGYQWIFYLVCAFAVASAAIVTLINPKEIDHEKARGGEDPANRCEPIAFRDLLKQ
                     RSLVIFLVSVVLFHFGNAAMLPMAGQVLAQTHKGEDTIALSACIIAAQFVMVGVAWAV
                     GQASARGVGRKPIFLVALAVLPVRGVLFSFTTSPYAVVGIQLLDGVAAGIFGVISVLI
                     ASDLMRGTGRFNLAQGLTALAVGIGATLSNATAGYVVQWFGYPSGFLYLAVIAGCALA
                     FFAVLMPETKESEAKPSVSGASVPAGAA"
     gene            complement(1927..2613)
                     /locus_tag="VGC82_18445"
     CDS             complement(1927..2613)
                     /locus_tag="VGC82_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425305.1"
                     /translation="MGTRHAFLVAALIAGATGIAAAQDAATFDPSQLPAIPGKVAQYS
                     LTPRGDVDGLILTDGTEVHLPPHLGTQLVYAVKPGDAVTVRGLRARAIPMVQAMSISN
                     DATGTTVSDNGPGGPRGPRGAEQPLTAEGRIKAQLHGPNGDLNGALLDDGTIVRLPPT
                     EAQRMAANLTAGAPLYVQGNGFAGPLGRVIEATSIGPNQGQLAQIAVPPVPPGPGHRP
                     PPPPPGRAPG"
     gene            complement(2689..4089)
                     /locus_tag="VGC82_18450"
     CDS             complement(2689..4089)
                     /locus_tag="VGC82_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0425306.1"
                     /translation="MATILIVDDDTALRQALATALSDLGHFPAEAADGETALAWLSRQ
                     QADAVLLDLRMPKMDGMEVLRRIRSGPGAPPVAVLTAVPTSDNTIEAMRLGAVDHLAK
                     PIGREGLKALLSRMLPDPSVGSRGAVAVASAREGELIGSSAAMRDVQKSIGLLADSDA
                     TVLLLGETGTGKEVVARAIHQHGKRTNAPFVPVNCAAIPAELLESLLFGHVRGSFTGA
                     VADRAGSFREAQDGTLFLDEIGDMDPAMQAKLLRALQERVITPVGGKPLPIDVRVIAA
                     THRDLVQAVRDGKFREDLYFRLGVVPLNLPPLRQRLADIIPLAEYFLSLSAPTGAVQQ
                     LSADAAVRLLAHKWPGNVRELLNAMKRVATLVRRPVITAADLDFLSTPPGGEAVDWLA
                     GTLPEAVDRLEIAMIRGALDACAGNRARAAEKLGIRRQLLYQKLARYGLEVSQNETAS
                     VPEADSDAEILPPPAQ"
     gene            complement(4074..5477)
                     /locus_tag="VGC82_18455"
     CDS             complement(4074..5477)
                     /locus_tag="VGC82_18455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0425307.1"
                     /translation="MVQIYRQSTQAQVGHAEAVIAHACDMIQERFGFYAAGWTSPVVI
                     QPDDRLRSEFTTVVSIALAHQEGVEGGIWQADSGPLAYAFPTYQGTGPKTDLPAAEHD
                     EIQAVNQRAARDEQRIERHVASKTQTLLLHACPLSGPIGGLTAWTMTRVQSAPRLLPL
                     QIGFGILFALMVLMSAWLGRTLLVWGRHVRGIELALARAGQDGMPAVPRTGETELDRI
                     IDALNDAGQRLAQARQEAEQMAIRVARAERLAGLGRVAAGVAHEIRNPIAAARLQGEN
                     ALAGDDDRRRQAITDMLGQIDRLDAHVAELLAMTQRVTPAPVRVDLAVFLADQVARHK
                     EIASAKRLAVSVRNARDTACLDPAVIGRVLDNLLSNAIRHAPVGGAVTLAVERDPGLL
                     TLTVEDTGGGVPPDMQDHLFEPFATGRADGTGLGLAIARELADAHGGRLVLRPSAPGS
                     GAAFSLSLPQDDAWPRS"
     gene            complement(5672..5953)
                     /locus_tag="VGC82_18460"
     CDS             complement(5672..5953)
                     /locus_tag="VGC82_18460"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEY0425308.1"
                     /translation="MQATLKDYVIAQSDDIVEAGGYAYFPPAATHLKWLEKSPKTEAD
                     YACPHGVQFYDVVLDGDRHERVAWSYESPQPKMQAVGGRFGFWKDVKVA"
     gene            6226..7107
                     /locus_tag="VGC82_18465"
     CDS             6226..7107
                     /locus_tag="VGC82_18465"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0425309.1"
                     /translation="MSVAVSESPAARSANLIPAVTAAFSFSITDILLKVVYASGMDVL
                     TLMSLRGLLVVAFFWPWLRLVPPARWHTKSERIIALGLGVLFAMTMFGLLKAISLLPV
                     SIAILAYFIYPLLTGIGAALTGVERLGWRGLLAAATAFLGLGLMLGQQIGELSIPGLA
                     FAFGAALCRVVSLLATRAFLRGADARVTTWLSMVPSALVFMAASAWVGDWQLPQAVWG
                     WGAFLGVTIASTLSTLLIYRSTNTIGAFRTALAMNLEPLLTLVFSMLLLGEILSPVQM
                     LGAAVMIASLCGFQFRR"
     gene            complement(7177..7326)
                     /locus_tag="VGC82_18470"
     CDS             complement(7177..7326)
                     /locus_tag="VGC82_18470"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY0425310.1"
                     /translation="MLMGLKQPLVAGQSFPLTLTFAHAPAVTVDVKVQPLGRASSASD
                     HGSSH"
BASE COUNT         1283 a         2395 c         2399 g         1259 t
ORIGIN      
        1 cgcgaggatc gatagtgccg acggcgctgc gaaaagtcgc agccattgca acagcggcgg
       61 catgtggctg ccgtaaatta ccaggttcgc cgggtttgaa atcggcagca cgaagcttgc
      121 cgcgttggcg atgaaggcgc agacgagcag gtagggcagc gcctccttcg cctttgccgc
      181 tttcaccacg gcggcgaccg cgggtgtcag caccaccgcg gtggcgtcgt tcgacaggaa
      241 caccgtcacg atcgtgccga cggcgaaaac caggctgaac agccgcgtcg gcgaaccccg
      301 cgccatgccg gccgccttgg ctgccagcca gtcgaacagg ccctcctgcc tggccagttc
      361 ggccagcagc atcatgccga tcaggaacag gtaaacgtcg ctgcccttcg ccacgccggc
      421 ccatgcgtcc gcaggcgcca gcaggcccaa cgccagtagc agcacggcgc cggccacgct
      481 ccagatgaac tcgggccacg aaaacgggcg gaagatcacg ccggcggtgg ccgcggcggc
      541 gatcccccag atcggccatg gcaaagcggc gagcgacccg ctcatgccgc cccggccggt
      601 acggaggcgc cggagacgct cggttttgcc tccgactcct ttgtctccgg catcaacacg
      661 gcgaagaacg ccagtgcgca accggcgatc actgccaagt acaagaagcc agagggatag
      721 ccgaaccatt gcacgacgta gccggcggtg gcgttgctga gcgtggcgcc gatgccgacc
      781 gccagggccg tcaatccctg ggcaaggttg aagcggccgg tgccgcgcat gaggtcggag
      841 gcgatcagca ccgagatcac cccaaagatg ccggccgcca caccgtcgag cagctggata
      901 ccgacgaccg cgtatgggct ggtagtgaac gagaacaaca cgccccggac gggcagaact
      961 gccagggcca ccaggaaaat cggcttgcgt ccgaccccgc gggccgaggc ttgcccgacg
     1021 gcccatgcaa cgcccaccat cacgaactgc gcggcgatga tgcacgccga cagggcgatc
     1081 gtgtcctcgc ccttgtgggt ctgggccagt acctgcccgg ccatcggcag catcgccgcg
     1141 ttgccgaagt ggaacagcac gaccgagacc aagaagatga ccaggctccg ttgttttagc
     1201 aggtcgcgga acgcgatcgg ctcacagcgg ttggccgggt cttcgccgcc gcgagccttt
     1261 tcatggtcta tttccttggg attgatcaag gtgacgatgg cggcgctggc cactgcgaag
     1321 gcacagacaa ggtagaagat ccattgatag ccgaggtact ggcccaaggt tccggccagc
     1381 acggcggcgg tcaggttgcc ggcatggttg aagctctcgt tgcggctgac ccgcccatcc
     1441 agcagtcgcc gtcccaccag gccaagcgac accgcggcga ttgccggtgg aatgacggcc
     1501 gaggccgcgc ccagcattgc ctgtgccgcg atgaccgtcc agaaccgggg aaacagcacg
     1561 atcagcagac agccgagccc caccatcagt cccgacccgg cgatcagaag tcgcttcttg
     1621 cggcttccgt ccaccagcag cccggccggt atctggcaaa tcgccgcggc gatcgaagat
     1681 gcgcccatgg caagtccgat cgccccggaa tcccaatgct gggcaccttt gagatagatc
     1741 gacaaatagg ggccgacgcc atcgcgcacg tcggccatca ggaaattgat gccgtccagc
     1801 ccgcgccagg agcgagagga cggatgctgg gatgaagtat tcaccgtgtc gtccgttccg
     1861 gttggccgga gccgagagtg cggaaatcgt ctcggttccc gatgtggatc aatggcggaa
     1921 tgttggttac ccgggggctc gcccaggcgg tggcggtggc ggacgatgcc ccgggccagg
     1981 cggaaccggc ggtaccgcga tctgggcgag ctggccctgg tttgggccga tcgatgtcgc
     2041 ctcgatcacg cggccgagcg gaccggcgaa accattgccc tggacataca gcggcgctcc
     2101 cgctgttagg ttggcagcca tgcgctgggc ctcggttggc ggcaggcgga cgatggttcc
     2161 gtcgtccaac agcgcgccgt tcaggtcacc attgggcccg tggagctgag ctttgatgcg
     2221 accttccgcc gtcaaaggtt gttcggcgcc ccgaggccca cgtggccccc cgggaccatt
     2281 gtcgctcaca gtggtgccgg tcgcgtcgtt gctgatcgac atggcctgca ccatcgggat
     2341 ggccctggcc cgcaggcccc gtaccgtcac ggcgtcgcca ggcttcactg cgtaaaccaa
     2401 ctgggtgccg agatgcggcg gcaggtgcac ctcggttccg tcggtcagga tcagtccgtc
     2461 cacgtcgccg cgaggcgtca ggctgtattg cgcgaccttg ccggggatcg ccggcaactg
     2521 gctgggatcg aaggtcgcgg catcctgcgc ggcagcgatg ccggtcgcgc ccgcgatcag
     2581 cgccgcgacc aggaaagcgt gtcgtgttcc cattgatgtc ctccgtcttc agggcatgaa
     2641 gcccgaggga ggaacagcaa gcagcgtgcc attcgtaagt gattggaatc actgggctgg
     2701 cggaggcagg atttcggcgt cgctgtctgc ttcggggacg gaagcggtct cgttttggga
     2761 cacctccaac ccgtagcgcg caagcttctg gtaaagcaac tgcctgcgga tccccagttt
     2821 ttccgctgcc cgtgcgcggt tccccgcaca agcatccagc gcgccgcgga tcatcgcgat
     2881 ctccaaccgg tccaccgcct ccggcagtgt cccggcgagc cagtcgaccg cttcgccacc
     2941 cggtggggtc gaaaggaaat cgagatcggc ggcagtgatg accggccggc gcaccaacgt
     3001 tgccacgcgc ttcatggcgt tcagcaattc gcgcacattg cctggccact tgtgggcgag
     3061 tagccgaacc gcggcgtcgg cactcagctg ctgtaccgca cccgtcggtg ccgacagcga
     3121 caggaaatac tcggccagcg gaatgatgtc ggccagtcgt tggcgcagcg gcggcaggtt
     3181 cagcggcacg acgcccagcc gaaaatacag atcctcgcgg aatttgccgt cgcgcaccgc
     3241 ctgcacgagg tcgcggtgcg ttgccgcgat gacccgtacg tcgatcggta acggcttgcc
     3301 gccgaccggc gtgatcacgc gttcctgcag cgcgcgcagc agtttcgcct gcatggccgg
     3361 gtccatgtcg ccgatctcat cgaggaacag cgtgccgtcc tgggcttctc ggaaagaccc
     3421 ggcacgatcg gcgaccgctc cggtaaagga tccgcggaca tggccgaata gcaggctttc
     3481 gagcagttcc gccgggatag cggcacaatt gaccggcacg aatggagcat tcgtccgctt
     3541 gccgtgctgg tggatggcgc gcgcgacgac ctctttgccg gttcccgtct cgcccagcag
     3601 cagcacggtc gcgtcgctat cggccagcag tccgatggac ttctggacgt cccgcatggc
     3661 ggcactcgat ccaatcagtt cgccctcacg cgcggacgct acggccaccg cgccgcggga
     3721 tccgacggat gggtcgggca gcattcggga cagcagtgcc tttaaacctt cgcggccgat
     3781 cggcttggcg aggtgatcca cggcacccag ccgcatcgcc tcgatcgtgt tgtcgctcgt
     3841 tggaacggcg gtcagcaccg cgacgggcgg cgcgccgggt ccgcttcgga tgcgccgcag
     3901 tacctccatc ccatccattt tcggcatccg cagatccaac agaaccgcgt cggcttgctg
     3961 gcgggagagc caggcgagcg ccgtctcgcc atcagccgcc tcggccggaa aatgcccgag
     4021 gtcgctcaga gcggtcgcga gcgcttgtcg cagcgcggtg tcgtcatcga cgatcaggat
     4081 cgtggccatg catcatcctg cggcaagctc agcgagaacg cagcgccgct gccgggggcc
     4141 gaaggacgca ggaccaaccg gccgccatgt gcgtcggcca gttcgcgcgc aatggccaat
     4201 cccagaccgg taccatcggc acggccggtg gcgaacggct cgaacagatg gtcttgcata
     4261 tccggaggaa cgccgccgcc ggtgtcctcg acggtcagcg tcagcaggcc gggatcgcgt
     4321 tcgaccgcaa gcgtgacggc accacccacc ggcgcatgtc gaatcgcgtt tgacaacagg
     4381 ttatccagca cacggcctat cacggcggga tcgaggcagg cggtatccct ggcattgcgc
     4441 acggaaaccg ccaaccgctt cgccgacgcg atttccttat gccgcgccac ctgatcggcc
     4501 agaaaaacgg caaggtccac ccgtaccgga gccggagtga cgcgctgtgt catcgccagc
     4561 aactccgcca catgcgcatc caggcggtcg atctggccga gcatgtcggt gatggcctgg
     4621 cggcggcggt catcgtcgcc ggccagcgcg ttctccccct gcaggcgcgc ggcggcgatc
     4681 ggattgcgga tttcatgcgc gacacccgct gctacgcgac ccaggccggc caggcgttcc
     4741 gcccgggcaa cgcgaattgc catctgctcc gcctcctgcc gagcctgggc caaccgttgc
     4801 ccggcgtcgt tgagagcgtc gatgatccga tccagttccg tttcgccggt gcgtggcact
     4861 gccggcatgc cgtcctggcc ggcccgggcg agagccagtt cgatgccgcg gacatgacgg
     4921 ccccagacga gaagcgtgcg gccgagccat gccgacatca ggaccatgag tgcgaacagg
     4981 atgccgaagc cgatttgcaa cggtaacagc ctgggcgccg actggacgcg ggtcatcgtc
     5041 caggcggtca ggcccccgat cggcccgctc aggggacagg cgtgcagcag caaagtctgg
     5101 gttttggagg caacgtgccg ctcgatccgt tgttcatccc gggcggcgcg ctggttcacc
     5161 gcctggattt cgtcgtgctc cgcagccggc agatcggttt tcgggccggt accctgatag
     5221 gtcgggaagg cgtatgcgag cgggccggaa tccgcttgcc agatgcctcc ttcaacgccc
     5281 tcctgatggg ccagggcgat gctgacgacg gttgtgaatt cagagcggag gcgatcatcc
     5341 ggctggatga ccaccgggct tgtccatccg gcggcgtaga acccaaaccg ctcctggatc
     5401 atgtcgcagg catgcgcaat gaccgcctcg gcgtgcccca cctgtgcctg cgtggactgg
     5461 cggtaaatct ggaccaacag gatgccaacg gcgacgctgg caaccagaga gagcgaccac
     5521 aggccgagca gtcgggcttt cagcgagtgc aggacctagc gcctttcaaa cagccccccg
     5581 ctgtttcagg acagggccgc ttcaagacag ggccgcttca agacagggca ccttcaaggc
     5641 acacacccga gccttccggt gctctcccgg atcaagccac cttgacgtcc ttccaaaatc
     5701 cgaagcggcc gccgaccgcc tgcattttcg gttgcgggct ctcgtacgac caggcaacgc
     5761 gttcgtgccg gtcgccgtcc agcaccacgt catagaattg aacgccatgc gggcaggcat
     5821 agtcggcttc ggtcttcggc gacttttcca gccatttaag gtgggtcgcg gcgggtggga
     5881 agtaagcgta gccgccggct tcgacgatgt cgtcgctttg ggcgatcacg taatccttca
     5941 gtgttgcttg cacgagagaa cctcctggat gggagaaaga gataggcgac acctaagctg
     6001 gattcgtcaa caaaatgtgt taacggaaag gcaagagcat cgcctgacgc ggtgcctctc
     6061 taagcttccg gctacagccg gatacttctc gaagaaattc caaggctacc gtcgcaccgc
     6121 ggtccatggc aacctacggg caggcgcttg cttcgggcgc ttgctcggga gacgccgcaa
     6181 aatgctgacc aaccggcccc gcacgggcac ggccggacgt cccgcgtgag cgtcgccgtc
     6241 agcgaatcgc ccgccgcacg atcggccaac ttgatcccgg ctgtgacagc ggcgttttca
     6301 ttcagtatca ccgacatcct gctgaaggtg gtttatgcct ccggcatgga cgtgctgaca
     6361 ttgatgtcgc tccgcggcct gctggtggtg gcatttttct ggccatggct gcggctcgtg
     6421 ccgcccgcgc gttggcacac gaaaagcgag cgaatcatcg ccctgggcct cggcgtactg
     6481 ttcgccatga cgatgtttgg cttgctgaag gcgatctcgc tgttgccggt gtcgatcgca
     6541 atcctcgcct attttatcta tcccctgctc accggcatcg gcgcggcgct taccggggtg
     6601 gagcgtctcg gctggcgcgg cctgctcgcc gccgccacgg ctttcctggg cctgggcctt
     6661 atgctgggtc agcaaatcgg cgaactgtcg ataccgggcc tggcctttgc gtttggcgcc
     6721 gcgctctgcc gggtggtgtc cctgctggca acccgagcct tcctgcgagg cgccgatgcg
     6781 cgggtgacga catggttgtc gatggtgccc tccgccctgg tgttcatggc ggcatcggcc
     6841 tgggtggggg attggcaact gccgcaagct gtttggggct ggggagcgtt cctgggagtc
     6901 acgatcgcca gcaccttgtc aaccttgctg atctatcgct cgaccaacac gatcggtgcg
     6961 tttcgaaccg cgcttgcgat gaacctggag ccactgctga ccctggtttt cagcatgttg
     7021 ttgctgggcg agatcctgtc gccggtgcag atgttaggag cagcggtgat gatcgcgtcg
     7081 ctgtgcggct tccagttccg ccgctgacga taaccgcggc gaccggcgtt ggctcgtgct
     7141 ggccgttgcg agatcggcga cgctggctgg ttcaggtcaa tgcgaggaac cgtggtcgct
     7201 cgcggaggag gcgcggccta atggctgaac cttcacgtcc acggttacgg caggcgcgtg
     7261 ggcaaacgtc agggtcaaag ggaagctctg accggccacc aaaggctgct tcaatcccat
     7321 cagcataatg tgcagg
//
LOCUS       DASWQN010001079         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1820558, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001079 DASWQN010000000
VERSION     DASWQN010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820558"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1299)
                     /locus_tag="VGC82_18475"
     CDS             complement(136..1299)
                     /locus_tag="VGC82_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEY0425311.1"
                     /translation="MRFADSIFASLLKPIDRRQFQKIVDRHDGDAYDKSFRSWDHLVA
                     LVYAQLAGVQSLRALETGFNANPQHHYHLGTAGFSRSTLSDANARRPAGIFAQRFATL
                     AASADRRTRREGEEMVRLIDSSPVPLGKLCKWTTWNGRIRGMKLHVIYDPVADVPSCV
                     EVTPANINDVEVGARTAITAGTTYVFDKGYCRFDWWKKINDCGAFFVTRAKVNMRLRA
                     SRRRPLRKRKGDGFKVLADDEVVLASKGNASLPIPLRRIKIRRDNGGVITLITNDLQR
                     SAVEIAALYKSRWQIELLFRWIKQHLDIRKFLGVNENAVRLQVLAAMIAYLLLRIAAR
                     INCIAMLPLRLAELVSQFLFTRRSIGTITKPPPVNPSTRQHRVSPDQLELCYA"
BASE COUNT          300 a          467 c          504 g          321 t
ORIGIN      
        1 tctgccgccg ctcgatcagc ctggcggcga tctgttcggc gatcggaatg aggtcttgca
       61 tcaggtcaat cctctctagg gcgtcatcac tcgcgttcgt cgtccccgcg aaggcgtaag
      121 gctgtccggg gaaaatcaag catagcacag ctccagctgg tctggagaga cgcgatgctg
      181 tcgtgtgctg ggattgacgg gtggcggttt ggtgatggtt ccgatggatc gtctcgtgaa
      241 caggaactgg ctgacgagtt cggcgagccg gagtggaagc atggcgatgc agtttatccg
      301 cgccgcgatg cgcagcagca gataggcgat catggcggcg aggacttgca ggcggaccgc
      361 attttcgttg acgccaagaa acttccggat gtcgagatgc tgcttgatcc agcggaacag
      421 cagttcgatt tgccagcggc tcttgtagag ggcggcgatt tcgacggcgc tgcgttgaag
      481 atcattggtg atgagcgtga tgacaccgcc gttgtcgcgc ctgatcttga tgcgccggag
      541 cgggatcggc aggctggcgt ttcccttgct ggcgagcacc acctcgtcat cggccagcac
      601 cttgaatcca tcgcccttgc gcttgcgcaa ggggcggcgg cgactggcgc gaaggcgcat
      661 gttcaccttg gcccgggtta cgaagaaagc cccgcaatca ttgatcttct tccaccagtc
      721 gaagcggcaa taacccttgt cgaacacgta ggttgtcccg gccgtgatcg ccgtccgggc
      781 gcctacctca acgtcgttga tattggcggg agtaacttcg acacagctcg gcacgtcggc
      841 cactggatcg tagatcacgt gcagcttcat gccgcgaatg cgtccgttcc aggtggtcca
      901 tttgcagagc ttgccaagcg ggaccggact ggagtcgatc aggcgcacca tctcttcgcc
      961 ttcacgccgc gtccggcgat ctgcggaggc cgccagcgtg gcgaaccgtt gcgcaaaaat
     1021 accggccgga cgccgtgcat tggcatccga cagcgtcgag cgggagaacc cggcggtgcc
     1081 cagatgatag tgatgctgcg gattggcatt gaagccggtc tccagcgccc gaaggctctg
     1141 aactcccgcc agttgcgcgt agaccagcgc caccagatgg tcccagctcc tgaacgactt
     1201 gtcataggcg tcgccgtcat gccgatccac aatcttctga aactgccttc gatcaatcgg
     1261 tttgagtagg ctggcaaaaa tgctatcggc aaatcgcatg ctcggctcct tctgtgtctc
     1321 gacaaccagg aagaaccgat ttgcctcggc acggccgagc atgcactgcg acatggtgaa
     1381 tcattccccg gacagcccta cggaagagcg gggagaggtt aagaaggacg ccgcgtccgg
     1441 ggcacgcgca taaactaatg ccgcgccatc gccccgtgcg cgtgcttgtc gatcgcgtcg
     1501 atgatttgcg gccacatcgg gatcggaatg gcgtggccca tgccgtcgac catcatcagc
     1561 ttggcgccgg ggatcgaggc cgcagtgtcc tt
//
LOCUS       DASWQN010001080         3873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_763052, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001080 DASWQN010000000
VERSION     DASWQN010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763052"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VGC82_18480"
     CDS             complement(<1..438)
                     /locus_tag="VGC82_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010506597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY0425312.1"
                     /translation="MRSGKTITRVDIYIFRQVLFALVVATVGLTALIWLTQSLRFVDL
                     VVNRGLSFFVFLHLTSLLIPSLIEVMLPITTYVVIQFVYQRMSTDRELTVMRAAGLSP
                     WSLARPALAVALLAMLTGYGLSLSIVPASMADFKEFQWEIRNRL"
     gene            760..2046
                     /locus_tag="VGC82_18485"
     CDS             760..2046
                     /locus_tag="VGC82_18485"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0425313.1"
                     /translation="MPILTWSGCRAEATSDGAGYGRWLVLLAMGATMLLPVALLYARA
                     IGDGVLTLIAVLFLAGHWAGGDWHWLRTKWTRLALLFWGWMLLCTVVSGNWPAIEQAL
                     ASLRFFLFAAALENMVLADAVARRRLWYVVLAAALWIFVEVWQQYLLGVNVFGYPRWA
                     DGALTGPFRGPTAGSAYLSVFFPAFLPLCFLLLRQPNIVGRILGIGVPVVAAATMILI
                     GQRMPALLMAVGLCASGLLFRQFRLPLLLTFAISGLVLALTPILSPPTFEKLVVHFTD
                     QVQHFWASPYGLIFARAVTMIQANPWMGLGWDGYRNGCMRPEYLSGVSWLTVSDPASA
                     VGCNLHPHNYWLQVATSAGLPGVVLFGSLVVVWLRRIAGAAGYLSNDRRAALLISVFV
                     SMWPVASATSLFTVPNAGWLFLMVGWGLAEARSADS"
     gene            complement(2171..3298)
                     /locus_tag="VGC82_18490"
     CDS             complement(2171..3298)
                     /locus_tag="VGC82_18490"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0425314.1"
                     /translation="MDAPLIIGGGPAGTAAAITLARAGHRPILLERNAGPTDKVCGDF
                     LSIDAIQQGQALGVDFAALGAAAIHRVRLVHRDSIVDTRLPFAALGLSRRTFDAALLR
                     LAVNAGALVHTGQAVRHLTPKADRWRVQTGSQVFDAATVFLATGKHDLRDAPRRRGEQ
                     GAIGMKMYLALAAEPARALEGATELTLFPGGYAGMQCVENGQAVLCIAVQKPAFAAYG
                     ADWSSLIAAIGQSSRRFATMMTGSHPLLPRPLAVAGIPYGYQARKMTIRQGLFRLGDQ
                     AAVIPSLTGDGMAIALHSGQQAARAWLTDHNAAAYQQALTQTLTPQMRLAGLLHGAGT
                     NALTQNAAIHILRWFPGLLRHAASRTRLPNAARDVLRTVRS"
     gene            complement(3319..>3873)
                     /locus_tag="VGC82_18495"
     CDS             complement(3319..>3873)
                     /locus_tag="VGC82_18495"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0425315.1"
                     /translation="PMLSWLAKRAKGDFSLLDVACGHGDALRKIRQRFPHAKLTGIDL
                     NPWATRAATEATDPAAHIEFINDDAFAYRRGRKPDFIITSQFTHHLTDDQVVAFLRWV
                     QGNAAQGWFISDIHRHAIAYYGFPILARLAFWHRLVREDGRISVARAFIRAEWQAMLL
                     QAGITEQQASVSWYIPFRLCVATR"
BASE COUNT          657 a         1142 c         1335 g          739 t
ORIGIN      
        1 aagacggttg cggatttccc attggaattc tttgaagtcc gccatcgagg ccggaacgat
       61 cgacaggctg aggccgtagc cggtcagcat cgccagcagc gccaccgcca atgcaggccg
      121 ggcaagcgac cacggtgaca ggccggcagc gcgcatcacc gtcaattccc ggtccgtcga
      181 catgcgctga tagacaaact ggatgacgac ataggtggtg atcggcagca tcacctcgat
      241 aaggctggga ataagcagcg aggtcagatg taggaacaca aagaacgaga ggccgcggtt
      301 gaccacgagg tccacaaacc gcaaggactg ggtcagccag atcagcgccg tcaggcccac
      361 cgtggcgaca acgagcgcga atagcacctg acggaagata taaatatcca cacgtgtaat
      421 cgtcttgccg gagcgcatgc tgctttctgc ggcaggcttg gacatgatgc aaccggggat
      481 ttgggacgcc gccgggcgtc gagcgcgatc gcctcgccag gaagcgatca tcgcggcaca
      541 tcccggatga catcgcgtcg gaacggcgtt atgggacgct gcgcgccatg cggatcgccg
      601 tagcttcgga taaggcggcg ttttgaggcg acccgccgtt tacgactccg cttctatgcc
      661 ggatgaaaga tttctctacg ccttgcgaag tacaagaatg cgaaccgttc aaacccgcgg
      721 agcagcacgg cataggccat accgggcgac cgagcgccga tgccgatttt gacctggagc
      781 ggttgtcgcg ccgaagcgac atccgacggt gccggttacg gaagatggct tgtcttgctg
      841 gcgatgggcg ccacgatgct gctgccggtc gcgttgctgt acgcgcgcgc gatcggggac
      901 ggggtgttga ccctgatcgc ggtgctgttc ctggcgggtc attgggctgg cggtgactgg
      961 cactggctgc ggacgaaatg gactcgcctt gctctgctgt tctggggctg gatgctgctt
     1021 tgtacggtgg tgtccggaaa ctggccggcg atcgagcaag ccttggcatc gcttcgtttt
     1081 ttcctctttg ccgcggcgct ggaaaatatg gttttggccg atgcggtggc gcgccggcgg
     1141 ctttggtacg tggttctggc ggctgcgctg tggatattcg tcgaggtctg gcagcaatac
     1201 cttcttggcg tgaacgtttt cggttatccg cgttgggcgg atggcgccct gaccgggcca
     1261 ttccgcgggc cgacggcggg gagtgcctat ctgtcggtgt tctttccggc attcctgcca
     1321 ctgtgtttct tgcttctgcg tcagccgaac attgtgggcc ggatacttgg aatcggtgtg
     1381 ccggttgtcg ccgccgcaac gatgatcctt attggccaga ggatgccggc actcctgatg
     1441 gcagtcggat tgtgcgcgag cggattgttg ttccgccagt tccgcctgcc gttgctcctg
     1501 acctttgcga tcagcggact ggtgctggcc ctgaccccca ttctgtcacc gccgacgttc
     1561 gagaagctgg tcgttcattt caccgaccag gtgcagcatt tctgggcgag tccgtacggg
     1621 ttgatcttcg cccgggcagt cacgatgatc caggcgaacc cttggatggg gctgggctgg
     1681 gatgggtacc gcaacggctg catgcggccg gagtatctgt cgggcgtgtc gtggctgacg
     1741 gtgagcgacc cggccagcgc cgtcggctgc aacctgcatc cgcacaacta ctggctgcag
     1801 gtcgcaacga gtgccggcct tcccggtgtg gtgctgttcg ggtccctcgt cgttgtctgg
     1861 cttcggcgca ttgccggcgc ggctggctat ctgtccaacg accggcgggc ggcgctgctg
     1921 atcagcgttt tcgtgtcgat gtggccggtt gcttcggcta cgtcgctgtt cacggtgccg
     1981 aacgccggat ggctgttcct gatggtcggg tggggcctgg ccgaggctcg gtctgccgac
     2041 tcctgacaag gaggtgtcca gaaccgaacg tggtttgacg gcggcatggc tgcacggttg
     2101 cgttgcgctt cgtgctcagt caggactgat ttgggcggcc gtactgcgat gccggtgcga
     2161 acgctgaccg ctaggatcgt accgtccgca gcacatcgcg agccgcattg ggaagacgcg
     2221 tacggctcgc agcgtggcga agcaggccgg gaaaccagcg caggatatgg attgccgcgt
     2281 tctgagtcag ggcgttcgtg ccggcaccat gcagaaggcc cgccaggcgc atttgcgggg
     2341 tcagggtttg cgtcagggcc tgttgatagg ctgcggcatt atgatccgta agccaggctc
     2401 tggccgcttg ctgtccgctg tgcagcgcga tggccatccc gtcgccggta agggatggaa
     2461 tgaccgcggc ctgatcgccc aggcgaaaca gaccctggcg tatcgtcatc ttccgggcct
     2521 gatagccata gggaatgccg gccaccgcca gaggccgcgg cagcaacgga tgcgagcctg
     2581 tcatcatcgt ggcgaaacgc cggctgcttt gcccgatggc ggcgatcagg ctggaccagt
     2641 cagccccgta ggcggcgaac gctggttttt gcacggctat gcacagcacc gcttggccgt
     2701 tctccacgca ttgcatgccg gcatagccgc cgggaaacag ggtgagttcg gttgcgccct
     2761 ccaacgccct cgccggttcg gctgccaggg ccaggtacat cttcatgccg atggcgcctt
     2821 gttcgccgcg cctgcgagga gcatcccgca gatcgtgctt tccggtggcg aggaaaacgg
     2881 tcgcggcatc gaacacctgg ctgccggtct gaacgcgcca ccgatccgcc ttgggggtca
     2941 ggtgccggac cgcctgaccc gtatgcacga gggccccggc attcacggcc agccgcaaca
     3001 gcgccgcatc gaacgtacgg cgggagagac caagcgccgc gaacggcagg cgggtatcga
     3061 cgatgctgtc gcggtgcacc aggcgaacgc gatggatcgc cgcggcgccg agcgcggcaa
     3121 agtcaacgcc gagggcttgc ccctgctgga tcgcgtcgat gctgaggaag tcgccgcaaa
     3181 ccttgtcggt cggcccggcg ttgcgctcga gcaggatcgg ccggtggccg gctcgtgcca
     3241 gcgtaatcgc ggcggcggta ccggcggggc ccccacctat gatcagaggg gcgtccatat
     3301 tcaaaggaac acccacagtc accgcgtcgc gacgcacagg cggaacggga tgtaccagga
     3361 aacggatgcc tgttgttcgg tgataccggc ctggaggagc atggcctgcc actcggcgcg
     3421 gataaatgcc cgggcgacgg aaatccggcc atcctcgcgg accaggcggt gccaaaaggc
     3481 gaggcgggcg aggatcggga agccataata ggcgatcgca tggcggtgga tgtcgctgat
     3541 gaaccagcct tgagcagcgt taccctgaac ccagcgcaag aacgccacga cctgatcgtc
     3601 ggtcagatgg tgggtgaact gcgacgtgat gatgaagtcc ggtttgcggc cgcgccgata
     3661 ggcgaaggcg tcgtcgttga tgaactcgat gtgggcggcg gggtcggtgg cctcggttgc
     3721 ggcccgcgtg gcccaagggt tcaggtcgat tccggtcagc ttcgcgtggg gaaagcgttg
     3781 gcggatcttg cgcaaggcgt caccgtggcc gcaggcgaca tccagcagcg agaagtcccc
     3841 tttcgcgcgc ttggcaagcc acgacagcat cgg
//
LOCUS       DASWQN010001081         4291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_763120, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001081 DASWQN010000000
VERSION     DASWQN010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763120"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1341)
                     /locus_tag="VGC82_18500"
     CDS             complement(46..1341)
                     /locus_tag="VGC82_18500"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY0425316.1"
                     /translation="MTLALQLMGSFGIFLLLLAAGMAVPFAIAVPGVIYLLMQNGLTA
                     LKGIGLVTWGSMNSFTLTAIPLFVLMAELMQRSGLSFRIYRGLAKVVARLPGGLLQTN
                     IVGCALFASISGSSVATAASIGGVALPQLVQRNYDRRLSAGSLAAGGTLGILLPPSIA
                     MIIYGSFTETSVAKLFMAGVVPGIMLTLMFMTYIGVRSFWGGVVPRENATAAGQSWIS
                     ALADVVPFALLIGAIMGSIYAGWATPTEAASGGCLFALLLCVVWGELTWAHFTGAMHR
                     TVQICGNLLFIIYAAYVYAYAIGVAGVGESVTSWLVGLHLSHFQFFGALFVLYTVLGC
                     LVESIGMIVITVPLLYPVLGSYGIDPIWFGVILVMYVELGQISPPIGINLFVIQSIWN
                     GELSDVVTGTIPFHIIMFVLLALLTVFPGLALWLPNQMN"
     gene            complement(1346..1876)
                     /locus_tag="VGC82_18505"
     CDS             complement(1346..1876)
                     /locus_tag="VGC82_18505"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY0425317.1"
                     /translation="MSEAAFAAEAAEDRFHPAPTLLEKICMWICEIAIVAMGAIVILE
                     IVTRNLFGFSFEMSEELGGYIIVGISFLSLPVCQVYKSYHHVQFIQARLPLRMQALSH
                     LLFDLLSLLFCCVILWQLARFVATTYRSEDVAPTLLATKLWIPQALMPIGMFAAIISL
                     SRSAYGNWRRFRAGGV"
     gene            complement(1873..2856)
                     /gene="dctP"
                     /locus_tag="VGC82_18510"
     CDS             complement(1873..2856)
                     /gene="dctP"
                     /locus_tag="VGC82_18510"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY0425318.1"
                     /translation="MPRSIVRAVLVACLLFVAEAAHAVTTWECYVYNPLAKQPSVDAV
                     VRLIEEVKQKTNGELLINLHLGGNLPIKADSITAAVADNVVQFGDDGFATGTIPITGV
                     LRLPLLLQTPEDLSKAMEIVRPYLESAYGKRGIEVLGQYSYPFQVIWGRKKIAALTDI
                     KGLKLRVTSVEQGEFIRRFGGVALTMGSPDVAAALDRGVVDGGLTASSGGGLLWHDLL
                     KYRYAFPTSYVNSTYVVNKEAMDKLPPETQKILRDAAAAWSKWSVNEMTRLEDEVTAQ
                     FGREGMTLTTATPAEIAEATEKMRPYWGEWAKAHGADATAALTKIRAAVGR"
     gene            complement(2995..3927)
                     /locus_tag="VGC82_18515"
     CDS             complement(2995..3927)
                     /locus_tag="VGC82_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0425319.1"
                     /translation="MARTKRWLLFLVPVVAMCAAAWAWTASAPRPIFPLHHASNLEPG
                     DAGRGKLVFEAADCGSCHASPGQDDRLKLGGGMVLDSPYGPFHAPNISPDPNDGIGSW
                     TTADLANALLTGVSPEGEHLYPTFPYSSFAHMKLDDVRDLMAYLSSLPAVAGRTPPHE
                     LTFPLTIRRAIGLWKLLFLDRSPVVDDPGRDAAWNRGHYLVEALGHCAECHSTRNIFG
                     AVEDATRFAGGPDPSSVGYSPNITPSAIGTWSPEQISEALRTGVTPGLRVLGSSMADV
                     IYNAALLPPGDRDGIAAYIKTLPARPTPNPAASQ"
     gene            complement(3983..>4291)
                     /locus_tag="VGC82_18520"
     CDS             complement(3983..>4291)
                     /locus_tag="VGC82_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425320.1"
                     /translation="NPGSAWPLSEELKGTQDMDEQADEKNPPKEQGLPQRGPAQPAGH
                     DDPREQSVPRHTAGGAPTDAAHQDAGDGALTGSVPAGLTARELLDGSRDVGRDDPGTE
                     "
BASE COUNT          860 a         1336 c         1348 g          747 t
ORIGIN      
        1 gtcggtggtg gcaaatatgc cggtggatga caggtaatcc aacactcagt tcatctgatt
       61 cggcagccac aacgcgagac cggggaatac cgtcagcaat gccagcagaa cgaacatgat
      121 gatatgaaac ggaatcgttc ccgtgacgac gtcgctgagt tccccgttcc agatgctctg
      181 gatgacgaac aggttgatcc cgatgggcgg cgagatctgc ccgagttcga catacatcac
      241 aagtatcacc ccgaaccaga tcggatcgat gccatagcta ccaagcaccg gatagagcaa
      301 cggcacggtg atgacgatca tgccgatgct ttcgactaag catcctaata ccgtgtagag
      361 cacgaacaat gcgccgaaga actgaaaatg cgacaggtgc aagccgacca gccatgacgt
      421 gacgctttcg ccgacaccgg cgaccccgat cgcgtaagca taaacgtagg cggcatagat
      481 gatgaacagc aggttgccgc agatctgcac ggtgcggtgc atcgcgccgg tgaaatgcgc
      541 ccaggtcagt tcaccccaca ccacgcaaag cagcagcgcg aacaggcagc cgcccgatgc
      601 cgcctcggtc ggcgtggccc aaccggcata gatgctgccc atgatggcgc cgatcaacag
      661 cgcgaacggc accacgtcgg ccagcgcgga gatccagctt tgccccgcgg cggtggcgtt
      721 ttcgcgagga acgaccccgc cccagaacga tcggacgccg atataggtca tgaacatcag
      781 ggtcagcatg atgccgggga cgactccggc catgaaaagt tttgccaccg aggtttcggt
      841 gaaagatccg tagatgatca tcgcgatcga cggcggcagc agaatgccca gcgttccgcc
      901 ggcggcgagc gatccggcgg acaatcggcg gtcgtagttg cgctggacca gttgcggcaa
      961 ggcgacgccg ccgatcgacg cggcggtggc gaccgatgaa ccgctgatcg aggcgaacag
     1021 cgcgcagccg acgatattgg tctgcaacag cccacccggc aggcgtgcga cgaccttggc
     1081 caaacctcgg tagatccgga acgacaagcc gctgcgctgc atcagttcgg ccatcagcac
     1141 gaacagcgga atcgccgtca gggtgaagct gttcatgctg ccccaggtga ccaggccgat
     1201 gcctttcagc gcggtcagcc cgttctgcat cagcaggtag atcacgccgg gcacggcgat
     1261 ggcgaacggg accgccatgc cggcggccag cagcagcagg aagatcccga aggaccccat
     1321 caattgcaat gccagggtca tggtttcaga ctccgcctgc ccggaatcgc cgccagttgc
     1381 catacgcgga ccgggacaac gatatgatcg cggcgaacat gccgatcggc atcagcgcct
     1441 gtggaatcca cagcttcgtg gccagcagcg tgggcgccac atcctctgac cggtaggtcg
     1501 tggcgacgaa ccgcgccagt tgccacagta tcacgcagca gaacagcaac gacagcaggt
     1561 cgaacaacag atgcgacagc gcctgcatcc gaagcggcag gcgggcctga ataaactgga
     1621 cgtggtggta ggatttatag acctggcaga ccggcaacga caggaacgaa atcccgacga
     1681 tgatatagcc gccgagttcc tccgacatct cgaaggaaaa accgaacagg ttgcgggtga
     1741 ctatttccag aatgacgatg gcgcccatcg ccacgatggc gatttcgcaa atccacatgc
     1801 agatcttttc cagcaatgtg ggcgccgggt gaaaccggtc ttccgcggcc tccgccgcga
     1861 atgcagcctc gctcatcggc ccacggcggc gcggattttc gtcagggcgg cggtggcgtc
     1921 ggcgccgtgc gcctttgccc attctcccca atagggccgc atcttttcgg tcgcctcggc
     1981 gatttccgcc ggcgtcgcgg tggtcagcgt catgccttcg cggccgaact gggcggtgac
     2041 ttcgtcctcc agccgggtca tctcgttgac cgaccacttc gaccaggcgg cggcggcatc
     2101 gcgcaggatc ttctgtgtct ccggcggcag cttatccatt gcctccttgt tcacgacata
     2161 ggtcgaattc acgtaggagg ttgggaaggc gtagcggtat ttcagcagat cgtgccacag
     2221 caggccgccg ccggagctgg cagtcagccc gccatcgacc acgccgcggt ccagcgcggc
     2281 ggccacgtcg ggcgatccca tggttagtgc cacgccaccg aaacggcgga taaattcgcc
     2341 ctgttccaca ctggttaccc gcagcttgag ccccttgatg tcggtcagcg cggcgatctt
     2401 cttacggccc caaatcacct ggaacggata ggaatactgc ccaagcacct cgatgccacg
     2461 cttgccgtaa gcgctctcca ggtaagggcg gacgatctcc atcgctttgg aaagatcctc
     2521 cggcgtctgc agcaggagcg gcaggcgcaa tacgccggtg atcgggatgg ttcccgtggc
     2581 gaagccgtcg tcaccgaact gcaccacgtt gtcggcgacg gcggcggtga tgctgtcggc
     2641 cttgatcggc agattgccgc cgaggtggag attgatcagc agctctccat tggtcttctg
     2701 cttaacttcc tcgatcaggc ggaccaccgc atcgacggat ggctgtttcg ccagtggatt
     2761 ataaacgtag cattcccatg tggtgaccgc atgggcggct tcagcgacga acagcaggca
     2821 ggcgaccagc acggcgcgga cgatggatct tggcacgaga cgttcccttg attcccggat
     2881 cgaccttacc cggccctcgt cggttggcaa tgggatcgcg ccgaacgttg gcttggccgg
     2941 cgtcctcggg gggcagtctt ggtcgatgag cgggaatgga actttaccgc cagatcactg
     3001 gctcgccgct ggatttggcg ttggtcgggc gggcagcgtc ttgatatagg cggcgattcc
     3061 gtctcggtcg cctggtggca acagggccgc gttatagatg acatccgcca tcgaggaacc
     3121 gagcacgcgc agaccggggg tgacgccggt gcgcaaagcc tcgctgatct gctcaggcga
     3181 ccatgtgcca atcgcactgg gcgtgatgtt cggagaatag ccgacgctac tgggatcagg
     3241 accgccggca aaacgggtgg cgtcctcgac agcgccgaag atattccgcg tggagtggca
     3301 ttcggcgcag tgcccgagcg cttcgaccag atagtggccg cggttccagg cggcatcgcg
     3361 acccggatca tccacgaccg gcgagcgatc gagaaacaac aacttccaca aaccgatggc
     3421 gcggcggatg gtgagcggaa atgtgagctc gtgtggcggc gtgcgcccgg ccacagcggg
     3481 aagactgctc aggtaggcca tgaggtcgcg cacgtcgtcc agcttcatgt gcgcaaagga
     3541 tgaataggga aacgtcggat aaagatgctc accctccggc gagaccccgg tgagcagcgc
     3601 attcgccaga tcggccgtcg tccagcttcc aatgccatca ttcgggtcgg gcgagatatt
     3661 gggagcgtgg aacggcccgt aaggtgagtc cagaaccatc ccgccaccca gcttcagccg
     3721 atcgtcctgt cccggagacg cgtggcagga cccgcagtcc gcggcttcga aaaccagctt
     3781 gccgcggcca gcgtcgccgg gttccaggtt gctcgcatga tgcagcggaa agatcgggcg
     3841 gggcgccgaa gcggtccagg cccaagcggc tgcgcacatt gcgaccacgg gaaccagaaa
     3901 cagcagccaa cgcttcgttc gcgccatcgc tttttctgcg cttggatcag gcttgacgcg
     3961 gcattacgct gggcaccgcg gtctattcgg tgccgggatc gtctcgaccg acatcccgcg
     4021 acccgtccag cagttccctt gcggtcaacc cggcgggcac agagcccgtc aacgcaccat
     4081 cgcctgcgtc ctgatgggca gcgtcagttg gcgcaccccc tgccgtatgc cgcggaacgg
     4141 attgctcgcg cgggtcatcg tgtcccgcgg gctgggcggg gccgcgctgc ggcaatcctt
     4201 gctccttcgg ggggttcttt tcgtcagcct gctcgtccat gtcctgtgtc cctttcagtt
     4261 cttcacttaa cggccaggcc gaacccgggt t
//
LOCUS       DASWQN010001082         3811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2347711, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001082 DASWQN010000000
VERSION     DASWQN010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2347711"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VGC82_18525"
     CDS             complement(<1..218)
                     /locus_tag="VGC82_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425321.1"
                     /translation="MDDQTADAAAWQNNRDKADLYANLGHQIAGLQSDETDLIANLAN
                     ASAAIYHGLPFLNWAGFYLFRGTELVLGP"
     gene            complement(312..722)
                     /locus_tag="VGC82_18530"
     CDS             complement(312..722)
                     /locus_tag="VGC82_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0425322.1"
                     /translation="MITNEVSDGLAGILRTAIVELVRRDGPDLSARQLGVFLTCYLET
                     EAQTVRGLAAKLGVSKPAITRALDRLSEFDLVRRKTDPLDRRSVLVQRTATGMAFLRE
                     MRTILRDAASTLRITAEPAQARARTGNEPARATH"
     gene            complement(1325..2578)
                     /locus_tag="VGC82_18535"
     CDS             complement(1325..2578)
                     /locus_tag="VGC82_18535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY0425323.1"
                     /translation="MPSLCRDCCLVADAIPLRCPVCGGGRLVHHRSLLRLTIAHMDCD
                     AFYASIEKRDRPELQGRPVIVGGGTRGVVTAACYVARIYGVKSAMPMFKALKACPDAV
                     VIKPDFAKYTAASRQVRSLMNRLTPLVQPLSIDEAVMDLSGTEALHGAPPAVVLARFA
                     GSVEKQVGVTVSIGLAANRLLAKIAAGRGKPRGFTVIDAAEAASLLAPEPVRLLPGVG
                     AALARKLETLGITRLGQLQALAERDAARKLGDDGPALVRRARGEDARIVDPSRETKSI
                     SAETTFDRDLSAVADLERPLWLLCEKLARRLKEHGLGAGGVVLKLKTTRFALRTRAAR
                     FSSPTVLPDRLFDLARLLLAKEATGTDFRLIGIGASPLVPGSTADRGDLADSDTPRRA
                     AAQAAIDALRSRFGDGAIGRGRSLR"
     gene            complement(2580..3479)
                     /locus_tag="VGC82_18540"
     CDS             complement(2580..3479)
                     /locus_tag="VGC82_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0425324.1"
                     /translation="MTRHRAGIDLGGTKIEIAVLAPDGSEALRHRIPTPRGYQDTVAA
                     IASLVRESEARLGVTATVGIGIPGVISPATGLVKNANSIALNGHRFDRDISDALAREV
                     RVENDANCFALSEATDGAGAGFGVVFGVILGTGCGGGIVVQGKVHGGRHRVAGEWGHT
                     PLPWPSGIEVPGHPCWCGKHGCLETYVAGPSLARDCDGPGAHDAGSLPRRAASGDRAA
                     SEALDRHADRLARGLAAVINLLDPDAIVLGGGLSNMRHLYTELPRRIPLYAFSDTIAT
                     PILPNRHGDSSGVRGAAWLWPAA"
BASE COUNT          702 a         1222 c         1170 g          717 t
ORIGIN      
        1 ggacccagaa ccagttccgt accgcgaaac aggtagaatc cggcccagtt caaaaatggc
       61 aggccgtggt aaatggctgc cgaggcgttg gccagattcg cgatgaggtc ggtttcatcc
      121 gactgcaagc cggctatctg atgccccagg tttgcatata aatccgcttt gtcgcggttg
      181 ttttgccatg cggcagcgtc agctgtttga tcgtccatcg ccgcgtccct tattcaccgg
      241 cgcggacgta tcgtccggcc gatgcagtgc aatcaggagc ggccgggccg cgaactgccg
      301 cggcccggat ctcagtgagt tgcccgcgcc ggctcgtttc cggtgcgcgc acgcgcttgt
      361 gcaggttctg ccgtgatgcg cagggtagaa gccgcatcgc gaaggattgt ccgcatctcc
      421 cgcagaaatg ccatgcccgt cgctgtgcgt tgcaccagga cgctgcggcg atccagcgga
      481 tcggtcttgc ggcgaaccag gtcgaactcc gacagccgat ccagagcgcg ggtgatcgcc
      541 ggcttcgaca cgcctagttt tgccgccagc ccgcgaaccg tttgcgcttc ggtttccagg
      601 taacacgtca gaaaaacacc gagctggcgt gccgagaggt ctggtccgtc gcggcgcacg
      661 agctccacga tcgcagttcg tagtatgcct gctaagccgt cacttacctc gtttgtgatc
      721 atactcctca acctttcctg tcaattggcg accatctcag accaagatct atggccgcca
      781 acagatactg ctcatgtcgg cgaattcgtg tcgcgtaacg aattggtgta ccaaccgtat
      841 ttgttccggg ctttatattg tgattacgga ataatgttcc tggctgatat ggtgtaactt
      901 cgtattgatg ttccactctg ccggaggcgt ggcccgggtc aatggattcg gccggggcac
      961 gggacgatac gtcgatgctt cctgcgtccg cgagctaaaa cgccgagatc cccctttttg
     1021 gccaggaact agttatttcc actgattttg cgtcgctttg catcggagcg agggcattcg
     1081 gccaccctcg cagccacagt tcgggccatc gtacacgtgg atttgttatt tttcggtgac
     1141 gatcagatat tattaccgtt tcgtattctc gaaatgaaca gaactttatg agttaaaaca
     1201 catactaagg cgagtttgca cccaccgagg attaggcctg cgaaccggaa tcggcgagcc
     1261 ggcactggca gccacggcag tgaattcgtt tcggcggata tccgacgtaa accggaacgg
     1321 ttgatcatcg cagcgaacgc ccacgcccga tcgccccgtc tccgaaacgg ctgcgcagtg
     1381 cgtcgatcgc cgcctgggcc gccgcccgtc tgggcgtgtc agaatcagcc aaatcccctc
     1441 ggtcggccgt agaacccggc accaaagggc tggcacctat gccaatcaaa cgaaagtcgg
     1501 ttccagtcgc ttctttcgcc agcagtaaac gagccaggtc gaacagccgg tcaggcagca
     1561 ccgtcggact ggaaaatcga gccgcccgag tccgcaaagc gaagcgtgtg gtcttgagct
     1621 tcaagaccac gccgccggcc ccaagtccgt gttccttcag tctccgggcc aacttttcgc
     1681 acagcagcca aagcggccgt tccaaatccg cgaccgccga caggtcgcga tcgaaggttg
     1741 tctcggcgct gatcgacttg gtttcacgcg acggatcgac gatccgcgca tcctcgcccc
     1801 gggcgcgccg aaccagcgcc ggcccgtcat cgccaagttt tcgtgcggcg tcgcgttccg
     1861 ccagcgcctg caactgaccc aaccgggtga ttcccaatgt ttccagcttg cgcgccagcg
     1921 ccgcaccgac gcccggcagc aggcgtaccg gctccggcgc cagcagcgag gcggcttcgg
     1981 ccgcgtcgat cacggtaaag ccgcgcggct tgccccgccc ggcggcgatc ttcgccagca
     2041 gccggttcgc cgccagtcca atggaaacgg tcactccaac ctgtttctcc accgagccgg
     2101 caaagcgcgc caggacgacg gccggaggcg cgccgtgcaa ggcctcggtt ccagacaggt
     2161 ccatcaccgc ttcgtcgatc gataaaggct gcaccagcgg cgtcagccgg ttcatcaaac
     2221 ttcgcacctg gcgggatgcc gcggtgtatt ttgcgaaatc cggcttgatg acgacggcat
     2281 cgggacaggc cttgagcgcc ttgaacatcg gcatcgcact tttcacgccg tagatacggg
     2341 cgacatagca ggccgccgtg acgacgccgc gtgtgccgcc gccgacgatc acggggcggc
     2401 cctgaagctc cggccggtcg cgcttttcta tgctggcata gaacgcgtcg cagtccatgt
     2461 gcgcgatcgt caggcgcagc agcgaccggt gatgcaccag cctgccgccg ccgcataccg
     2521 gacaacgaag cggaatggcg tcagcgacca ggcagcagtc gcggcacagc gacggcatgt
     2581 caggcggctg gccagagcca ggcagccccg cgcaccccgg aggaatcccc atgccggttc
     2641 ggcaggatcg gcgtcgcgat cgtgtcgctg aacgcataca agggaatgcg gcgcggcagc
     2701 tccgtgtaga ggtggcgcat gttcgaaagc ccgcccccca gcacgatcgc atccgggtcg
     2761 agcaggttga tcaccgctgc cagtccgcgg gccagccggt ccgcgtgtcg gtccaaggct
     2821 tccgaggcag cgcggtcgcc ggaagctgca cgccgcggca ggctgccggc gtcatgggcg
     2881 ccggggccgt cgcagtcacg cgcaagggat ggccctgcga cgtaggtttc cagacagccg
     2941 tgcttgccgc accagcatgg atgacccggt acctcgatac ccgaaggcca cggtagaggt
     3001 gtatggcccc actcgccagc gacgcggtgt cggccgccat gaaccttgcc ctgcacgacg
     3061 atgccgccgc cgcaaccggt gccgaggatg acaccgaaca caacgccgaa gccggcaccg
     3121 gcgccatcgg tcgcctcgct caatgcaaag cagttggcat cgttctcgac ccgtacttcc
     3181 cgagcgagcg catcgctgat gtcgcggtcg aagcggtggc cgttgagggc gatggagttg
     3241 gcgttcttca ccaggccggt ggccgggcta atgacgcccg gaatgccgat tccaaccgtc
     3301 gccgtgacgc ccagtctggc ttcggattcg cgcacgaggc tggcgatagc tgctacagtg
     3361 tcctggtatc cgcgtggcgt ggggatgcgg tgccgcaacg cttcgctgcc atccggggcg
     3421 agcactgcga tttcaatttt agtgccgccc aggtcgatgc ctgctcgatg tcgcgtcaaa
     3481 ggttccgccg aataggtggg ccgaccgtca tgataccatc agtgttgcgt cgtgacacgc
     3541 atttgggttc cgtcggaagg catctgcccc ggctggatcc gacccccatt cacgatttgg
     3601 ccgactggac aaagtgtgcg tccacagcgg tggaggcggt gctcgtccaa acccggcgat
     3661 tgttgacacg cttgcttcgg gcgatccggc gcgagttcag cgcggtttgc gcacacaggt
     3721 cctgccggca cgtgccagga ccagaaatcg ggcaaacgac cggacaaaac gggtgccgtt
     3781 aacaggatct tagcttgtcg ccttttatag t
//
LOCUS       DASWQN010001083         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1688236, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001083 DASWQN010000000
VERSION     DASWQN010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688236"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..477)
                     /locus_tag="VGC82_18545"
     CDS             complement(19..477)
                     /locus_tag="VGC82_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425325.1"
                     /translation="MTDKRKKLSETARTLLTAAAMRADHLIQPPQLPIAAARQIARSL
                     LSLGLAEEVPTSDEAADLGWPVGDGGKSHALRATAVGLARIGEGADTAAASSAHEVVA
                     EPGAPLTQTASNAGNPATVLAPTTEPATTSRPAQDAQAVSGGPNGRETAA"
     gene            1079..>1768
                     /locus_tag="VGC82_18550"
     CDS             1079..>1768
                     /locus_tag="VGC82_18550"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0425326.1"
                     /translation="MANQSTGGDRRIDRRTVLQVGGALAAAGPAVLHAAHAQAQQRPP
                     GSPRVIIDAQVHAYAANTPERPWNSVPNWPQHVTGDEMVAAMDKVGVDGAIYISPFSM
                     YQYDGSYAASVRQAHPDRFAIVKPVNPEDPAVAEVIADWKKTPGTVGIRIIMTKESKR
                     APDDPGFDRILRAAVQHDFPVNVLFWGNIDAGIALIDRHSDARFIIDHMGITQPSVPP
                     APAQPWADLPKV"
BASE COUNT          320 a          551 c          562 g          335 t
ORIGIN      
        1 gacggcagcc gtggttgtct aagccgctgt ctctcggcca ttagggccgc ctgacacggc
       61 ctgagcatcc tgtgcagggc gggacgtggt ggccggttct gtggttggcg ccagcacggt
      121 cgccgggttg ccggcgttgg atgcagtctg cgtcaacggc gcccccggct cagccaccac
      181 ctcatgggcg gatgatgcgg ccgcggtgtc tgcgccctcg ccgatacggg ctagcccgac
      241 cgccgtggcc cgaagcgcgt gcgatttgcc gccgtcgcca actggccatc caaggtctgc
      301 ggcttcgtcg gacgtcggca cctcttcggc gagccctaaa ctcagcaacg accgtgcgat
      361 ttgccgtgcc gcagcgatcg ggagctgtgg aggctggatc aggtggtcgg ctcgcatcgc
      421 cgccgcggtc agcagcgtac gcgcggtctc ggacagcttt ttgcgtttgt cggtcatgat
      481 ggaactcctg gaagcggacc gatcaggcgc cgctaccggc gcgagccctg ccgggcgata
      541 cccggtcggg gtgcgatggg gacttgccgc gatcaggctg cgtgttcgcc ttcgtggaaa
      601 gcggcgttgc tgaccttgcg caggcctttc aggtagctgc ccaagcttcc gacattggcc
      661 caagtgatat catccggcgc ttggttaaaa tgctttgcgc tcaatgtggc gagacgaacc
      721 aggattgtgt cgatttcgca ctcccgggcg agaaatgctg caaggacatc gctgttatcc
      781 ggggttttcg catcggggtg gctccgtcct ggtgaagtca tattgcttta aactgcatcc
      841 gagccaagcg gataagttga tcatttcatt gctttggatg cccaatagcg agctaaccca
      901 agtagaaccc caccatccca gtccgccagc gatggctggt ggcggtgaga tgctgctaat
      961 tggtctccct gccaatcgtt cgggcgctca caatgaccgc ttccttgacc aaggctgaag
     1021 aaagtccaca atgttcaacg atataaagcg caatttatca agcgctgtgg agggagaaat
     1081 ggccaatcaa tcaactggcg gagacaggcg catcgacagg cgtaccgtgc tgcaggtcgg
     1141 gggggcactg gcggccgccg ggccagcggt actgcacgcg gcgcatgccc aggcgcaaca
     1201 gcgaccgcca gggtctcccc gcgtgatcat tgatgcccag gtccatgcct atgcggcgaa
     1261 cacgccggag cgaccctgga acagcgtgcc aaactggccc cagcacgtta ccggcgacga
     1321 gatggtggcc gcgatggaca aggttggcgt cgatggcgcg atctacatct cgccgttttc
     1381 gatgtaccaa tacgacggca gctatgcggc gagtgtgcgg caggctcacc ccgaccggtt
     1441 cgccatcgtc aagccggtca acccggagga tcccgcggtc gccgaggtga tcgccgactg
     1501 gaagaagacc ccgggcacgg tcggcatccg catcatcatg accaaggagt cgaaacgagc
     1561 gccagacgat ccaggcttcg accggatcct gcgcgcggcc gttcagcatg atttcccggt
     1621 gaacgttctt ttctggggca acattgatgc cggcatcgcc ctgatcgacc gccactccga
     1681 tgcacgcttc atcatcgacc atatgggcat cacgcaaccc agcgtgccgc cggcgccggc
     1741 ccagccctgg gctgaccttc ccaaagtg
//
LOCUS       DASWQN010001084         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1028377, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001084 DASWQN010000000
VERSION     DASWQN010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028377"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VGC82_18555"
     CDS             <1..301
                     /locus_tag="VGC82_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425327.1"
                     /translation="TMASFSFNTAAELFPAAIRKKKRAGFAYRRFGTAAEAVRFAIEE
                     LPADSLNGAYLQVDEARFDQSGIRNLYESQDFPLPRRPRAPEPAPAADKADANAA"
     gene            complement(323..874)
                     /locus_tag="VGC82_18560"
     CDS             complement(323..874)
                     /locus_tag="VGC82_18560"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEY0425328.1"
                     /translation="MSNDDIISPPPGRSRGARPGTTSRATFGDSEARGPIIDQEGREI
                     PSEALGNQFQSLRFEFGNAGPLFGNLTREQRLARLDAVAKLLDVAFVLPGTNIRYGID
                     GIIGLIPVVGDIVATALSLWLVREARALGAPWYVTARMLGNVAIQGVVGAVPVAGDAF
                     DVLFRANIRNVRLLRRWIDKQPH"
     gene            complement(1205..1618)
                     /locus_tag="VGC82_18565"
     CDS             complement(1205..1618)
                     /locus_tag="VGC82_18565"
                     /inference="COORDINATES: protein motif:HMM:NF036644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5413 family protein"
                     /protein_id="HEY0425329.1"
                     /translation="MKRYLFFAALGPLLGGFLLLFATTYMSGYWTHTNLAEVQKLFVV
                     FAKTLQYSYLFGIVPSLMLAAVDDIICHVKRISPAVRILVVGALAFIVAELLYGSRGP
                     DSGVVQLILYGLVGFVPGTVASWVSHEVIGKAAKA"
     gene            complement(1652..>2503)
                     /locus_tag="VGC82_18570"
     CDS             complement(1652..>2503)
                     /locus_tag="VGC82_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425330.1"
                     /translation="EEAGRYAPSQQLAPRPPRNANDREQRRRPKTGTVFPFKSAIIVG
                     IVLILVGAAILWGKSLVTAVGGLFKPSSTVVEAPKDSSPLAKPKIPDRVGQPSSSEQV
                     APVAQRVVLYDEDPADPKGKQYVGSVIWRTEQIKAAAGQKADLAVRADIEIPDRKFKM
                     TMSFRRNTDTSLPASHTAELTFVLPPDFAGGGVGNVPGILMKSNEQARGTPLAGLAVK
                     VTDGFFLVGLSNVEADRARNIQLLKERSWFDVPLVYVNQRRAIIAVEKGAPGERAFND
                     AFAAWGE"
BASE COUNT          465 a          849 c          770 g          419 t
ORIGIN      
        1 gaccatggct tccttcagct tcaacaccgc tgccgaattg tttcccgccg cgatccggaa
       61 gaaaaagcgg gcgggtttcg cctatcggcg atttggcacc gcggccgaag cggttcgctt
      121 cgcgatcgag gaattgccgg cggactcgct gaacggcgct tacctgcagg tcgatgaagc
      181 ccgctttgat cagagcggta tccgaaatct ctatgagagc caggatttcc cgctgccgcg
      241 ccgcccccgt gcaccggagc ccgcgcccgc cgccgacaag gccgacgcca acgctgcctg
      301 agcttgcgta agcgtggaag cgctaatgtg gctgcttgtc gatccagcgc cgcaaaagcc
      361 gtacattgcg aatgttggcg cggaacagga cgtcgaaggc gtcgcctgcg accggcaccg
      421 cgcctaccac cccctggata gcgacattgc ccaacatccg ggccgtcacg taccacggcg
      481 cgcccagcgc gcgcgcctcg cgcaccagcc acagcgagag cgcggtcgcg acgatgtcgc
      541 cgacgaccgg gatcaacccg atgatgccgt cgatgccgta acggatattc gtgccgggca
      601 gaacgaacgc gacgtcgagc agcttcgcca cggcatccag ccgtgcgagc cgctgctcgc
      661 gggtcagatt gccgaacaga ggtccggcat ttccgaattc gaagcgcagg ctctggaact
      721 ggttgcccag cgcttccgac gggatttcgc gcccttcctg atcgatgata gggccccgcg
      781 cctccgaatc accaaacgtg gctcgggagg tcgttccagg acgagccccg cgagagcggc
      841 ccggtggcgg cgaaataata tcgtcgttcg acatcgtcat cccaccgtaa cgcccgaaac
      901 tggcgcaagt tgcgtgatgc ggccgcgccg gccgcaaggc cgtccgtcct tcgcctgacg
      961 cccgggggct tgcctttcat ggccgctacc ggccccgtac cgccgatggc gtcagcccaa
     1021 aacggcggca aacgcagcgc ttgatcgttg aaccctacaa tccaacgcga cgtcgctgat
     1081 ccgcctgtgc atgcgccagt tatcggttgt ggccggagcg aaggcggacc tcgatgccgt
     1141 tgcgaccgcg agacaagtcg cggccgttcg tttgccatga acgttgcggc aacaggccgc
     1201 gtcatcaggc cttcgcggcc ttgccgatca cctcatgcga cacccatgat gcgaccgtcc
     1261 cgggcacaaa ccccaccagg ccgtaaagaa tgagctgaac aacgccgctg tccggccccc
     1321 gcgagccgta gagcaattca gcgacgatga aggcgagagc gccgaccacc agaatccgga
     1381 ctgccggcga aattcttttg acgtggcaaa taatatcgtc caccgcggcc agcatcagcg
     1441 aaggcacgat accgaacagg tagctgtatt gaagcgtctt ggcgaacacc acgaacagct
     1501 tctggacttc cgccagattg gtgtgggtcc aatagcccga catataagtt gtcgcgaaca
     1561 gcagcagaaa cccgcccagc agcggcccca gcgccgcgaa aaacaaatac cgcttcatgc
     1621 tgtgctgcgc gccctggctc ggacccgccc cctactcccc ccaggccgcg aacgcgtcat
     1681 tgaacgcgcg ctcgcctggc gcacccttct cgacggcaat gatggcccgg cgctggttga
     1741 catagaccag cggtacgtcg aaccacgagc gctccttcag aagctggatg ttgcgtgcgc
     1801 ggtcggcctc gacattcgac aggccgacca ggaagaagcc gtcggtgacc ttgaccgcca
     1861 gaccggcgag cggggtgccg cgcgcctgct cgttggattt catcaggatg cccggcacgt
     1921 tgccgacgcc gccacccgcg aaatccggtg gcaggacgaa ggtcagctcc gcggtatggc
     1981 tggccggcag cgaggtgtcg gtgttgcggc ggaacgacat tgtcatcttg aacttgcggt
     2041 cgggaatctc gatgtcggca cgcacggcga gatcggcctt ctggccggcc gcggccttga
     2101 tctgttcggt gcgccagatc accgagccga catattgctt gcccttggga tcggctggat
     2161 cctcatcgta cagcaccaca cgttgcgcca ccggcgcaac ctgttccgac gatgacggct
     2221 gtccaacccg atcggggatt ttcggcttgg cgagcggcga actatccttc ggcgcctcca
     2281 ccaccgtgga ggagggcttg aacaggcctc ccaccgcggt caccagcgac ttgccccaca
     2341 ggatcgcggc tccgaccagg atcagcacga tgccgacgat gatcgcgctc ttgaacggaa
     2401 acactgtccc cgtcttgggg cggcgccttt gctcgcggtc gttggcgttg cgcggcggcc
     2461 gcggcgcaag ctgttgcgac ggcgcgtagc gcccggcctc ctc
//
LOCUS       DASWQN010001085         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_897185, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001085 DASWQN010000000
VERSION     DASWQN010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897185"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VGC82_18575"
     CDS             complement(<1..1173)
                     /locus_tag="VGC82_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY0425331.1"
                     /translation="MASAPQERVPHRLIITLCTVGATLMQALDQTIANVALPYMQGSL
                     SATYDEITWVLTSYITAAAIMTAPVGWLASRFGRKYLFITCLIGFTVTSMMCGAAQSL
                     AQMVVFRLLQGVFGAALVPLSQSTMMDIYPIEQRGKAMAIWGVGVMVGPILGPTLGGY
                     LTEMYNWRYVFYINLPFGILATLGLLFFMPRSFGNFNLRFDWIGFAVLSLGIGGLQMM
                     LDRGQNQDWFTSTEVVVEAVLGSLGVYLFVVHMATAKKPFIPVMLFKDRNFAAGLLLM
                     GATGTILVSSSSLMAPWLQNLANYPVETAGLIMAPRGIGTMTAMLTGGRLSSKMDPRK
                     LMACGILALAWSMWEMTGWTPDVSQWTIVTTIMVQGAGLGFVFLPLQVLAFATLPPQ"
     gene            complement(1313..>1712)
                     /locus_tag="VGC82_18580"
     CDS             complement(1313..>1712)
                     /locus_tag="VGC82_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0425332.1"
                     /translation="TVTQVETVQRGMYLAAATAAFGLVSTDNVWVEANPKETELTHVK
                     PGDPVDVTVDTYPGRTWKAQVESIAPNSGSEFSVLPAQNTSGNWVKVVQRIPVRIKVE
                     RQEGDPELRAGMSVIAEIDTGHRRTWHDLY"
BASE COUNT          349 a          576 c          488 g          299 t
ORIGIN      
        1 ttgcggcggc aacgtcgcga acgccagtac ctgcaacggc aggaacacga agccaaggcc
       61 agctccctgc accataatcg tcgtcacgat cgtccattgt gacacgtcag gggtccagcc
      121 tgtcatctcc cacatcgacc acgccagcgc caaaatgccg catgccatca gcttgcgcgg
      181 gtccatcttg ctcgacagcc ggccgccggt cagcatcgcc gtcatcgtgc cgatgccccg
      241 cggtgccatg atcagccccg cggtttccac ggggtaattc gccaggttct gcaaccatgg
      301 cgccataagc gacgaactgg aaacgagaat cgtgccggtt gcgcccatca gcaacagccc
      361 ggccgcaaaa ttgcggtcct tgaacaacat gaccggaatg aacggcttct tggccgtcgc
      421 catgtggacc acgaacagat agacgcccag gctgcccagc accgcctcga cgaccacctc
      481 ggtggaggtg aaccagtcct ggttttgccc acgatccagc atcatctgca ggccgccaat
      541 cccaagcgac agcaccgcaa agccgatcca gtcgaaacgt agattgaagt tgccgaacga
      601 ccgcggcatg aagaacagca gccccagggt ggccaggatt ccaaatggca ggttgatgta
      661 gaacacgtag cgccagttgt acatctcggt caggtagccg ccgagagttg gccccaggat
      721 cggtccgacc atcacgccca cgccccagat cgccatggcc ttgccgcgtt gctcgatcgg
      781 atagatatcc atcatcgtgg attgcgacag cggcaccaga gccgctccga acaccccctg
      841 caacagccgg aagacgacca tctgcgccaa cgactgcgcc gcgccgcaca tcatggaggt
      901 gacggtgaag ccgatcaggc aggtgatgaa caggtatttt cgcccgaacc gagacgccag
      961 ccatcccacc ggcgccgtca tgatcgcggc ggcggtaatg taggacgtca ggacccaggt
     1021 gatctcgtca tacgtcgccg acaggctgcc ctgcatgtac ggcagtgcga cattggcgat
     1081 cgtctggtca agcgcctgca tcagcgtcgc gccgacggtg caaagcgtga tgatcagccg
     1141 gtgcggaacg cgttcctgag gtgccgaggc catgcgttca ggcgatcttg gaatcagttt
     1201 tcacaattcg gttcgtccag cgtggcgacg ccggagatat gcgacggcca agagccggcc
     1261 cccgaaccaa atcccggcgc ctgggcggtt gaaacacaat ccacagcatg gcttagtaca
     1321 ggtcgtgcca ggtgcggcga tgcccggtgt cgatctccgc gatgacgctc atgccggccc
     1381 gcagttccgg atcgccttcc tggcgctcca ccttgatccg taccggaatg cgctgcacca
     1441 ccttcaccca gttgccggac gtattctgcg ccggcagcac ggagaattcc gacccgctgt
     1501 tcggggcgat cgattccacc tgcgccttcc aggttcggcc cggataagta tcgaccgtca
     1561 catccaccgg atcgcccggt ttcacatgcg tcagttcggt ttctttcgga ttggcctcga
     1621 cccacacatt gtccgtcgaa accaggccga acgccgccgt tgccgccgcc aggtacatgc
     1681 cccgctgcac cgtttccacc tgggtgaccg tg
//
LOCUS       DASWQN010001086         2027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1028665, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001086 DASWQN010000000
VERSION     DASWQN010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028665"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..1976
                     /locus_tag="VGC82_18585"
     CDS             300..1976
                     /locus_tag="VGC82_18585"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0425333.1"
                     /translation="MTTDIANQTTELDALVVGAGFAGLYQLLCLRDRLGLSVKTIEAG
                     AGVGGTWYWNRYPGARCDSESHVYWYTFSPDLMREWEWSERYPGQPEIMRYLNHVADR
                     FDLKRDILFNTRVTAAQYDETANRWRVRTENGETFVAKFLITAVGCLSTANVPAFNGL
                     ESFSGEWYHTGQWPHEGVDFTGKRVGMIGTGSTGIQAAPVIAAAAEHLTVFQRTANYS
                     VPARNAPLTPEFRRYIKENAAEIRAVTRDTLNGMAFRIEERLAVETPPEEREAIYDAA
                     WERGGLQFRAAFKDMMVDKAANDTAAEFVKRKIRSIVKDPKTAAILSDIDHPYAAKRP
                     PIDTNYFETFNRANVTLVDVKATPIERISPAGVCTTQAEYPLDIIVFATGFDAMTGPL
                     LRMNIQGRDGIALKDVWEAGRATTLDCRSRVFRTCLRSPVRAARRCCATCRWRSSSTQ
                     TGSPIASRTCARTGWSGSKLDPRRWTSGSRRSMTSPARRCCRWRSIPGISAPIFRASP
                     RFSCRMQGEWFATGRSVTMSRRVAMKGSAWRRGSSFCSNMHDQHRFCDLG"
BASE COUNT          395 a          571 c          681 g          380 t
ORIGIN      
        1 tcagtcagat ccagatgatg cgctggagcg gcaagagttg ggacctgttc ggcgacgtgc
       61 tgtcgggtgg cgggtcgatt taggtctgga tgggctcgtc gttacaggaa gacggcaaga
      121 ggcattccgc cgaacgtttg caggccgcag gcggcgcagt gcgcctggct cgatccgaca
      181 tcatgagact cgggcgggaa acttgatgtt gagcctgatc caggggatat cgggtcggca
      241 gcgggacggc aaagccgctt gaccacggaa atggggaggc tcgggcaacg atggcgaccg
      301 tgacaacaga cattgcgaac cagaccacgg aactcgacgc cctggtggtc ggtgccggat
      361 ttgcgggcct gtaccaactc ctgtgcctgc gcgaccgtct cggcttgtcc gtgaagacga
      421 tcgaagcggg cgctggcgtc ggcggcacct ggtactggaa ccgctatcca ggcgcgcggt
      481 gtgattccga gagtcacgtg tactggtaca cgttctctcc cgacctgatg cgcgaatggg
      541 agtggtccga gcgctatccg ggccaaccgg aaatcatgcg ctacctcaac catgtcgcgg
      601 accggttcga tctgaagcgg gacatcctgt tcaacactcg tgtcactgcg gcgcagtacg
      661 atgagactgc caatcgctgg cgcgtccgga ccgaaaacgg tgagacattc gttgcgaagt
      721 ttttgataac cgccgttggc tgcctgtcca cggcaaatgt tccggccttc aacggattgg
      781 agagcttctc aggcgagtgg tatcacaccg ggcagtggcc gcacgagggt gtggatttta
      841 ccggcaagcg ggttggaatg atcggcaccg ggtccaccgg catccaggcg gcgccggtga
      901 tcgccgcggc ggcggagcat ctgacggtgt tccagcgcac cgcgaattac tcggtgcccg
      961 cgcgcaacgc gccgctgacg cccgagttcc ggcgctacat caaggaaaac gccgccgaga
     1021 tcagggcggt gacccgcgat acactaaacg gcatggcgtt ccggatcgag gaacgcctgg
     1081 ccgtcgaaac cccgccggaa gagcgcgagg cgatctacga tgctgcgtgg gagcgcggcg
     1141 ggttgcagtt ccgcgctgcg ttcaaggaca tgatggttga caaggcggcg aacgatacgg
     1201 cggcggagtt cgtcaaacgc aagatccgct cgatcgtgaa ggacccgaag acggcggcta
     1261 tcctgtcgga tatcgatcat ccctatgcgg caaagcggcc gccgatcgac accaattact
     1321 tcgaaacgtt caaccgtgcg aatgtaacgc tggtggacgt gaaggcgacg ccgatcgagc
     1381 gtatcagtcc cgctggggtg tgtaccacgc aggcagagta tccgttggac atcatcgtct
     1441 tcgccaccgg cttcgatgcc atgaccggtc ctttgctgcg gatgaacatt cagggacgcg
     1501 acggcattgc cttgaaggat gtgtgggagg ctgggcgcgc aactaccttg gactgcaggt
     1561 cgcgggtttt ccgaacctgt ttacgatcac cggtccgggc agcccgtcgg tgctgtgcaa
     1621 catgccggtg gcgatcgagc agcacgcaga ctggatcgcc gattgcatct cgtacatgcg
     1681 cgcgaacggg ctggagcgga tcgaagctcg acccgaggcg gtggacaagt gggtcgcgga
     1741 ggtcaatgac gtcgccagca agacgctgct gccgctggcg aagcattcct ggtatctcgg
     1801 cgccaatatt ccgggcaagc ccaaggtttt catgccgtat gcagggggaa tggttcgcta
     1861 ccgggagatc tgtaacgatg tcgcggcgcg tggctatgaa gggttcagct tggcgtagag
     1921 gcagttcctt ctgctcgaac atgcatgacc aacaccgctt ttgcgacctc ggttagggtt
     1981 tgctgcggca cgatcgcttc gaaagcgacc tcaaggccgc catggtg
//
LOCUS       DASWQN010001087         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_763795, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001087 DASWQN010000000
VERSION     DASWQN010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763795"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VGC82_18590"
     CDS             complement(<1..376)
                     /locus_tag="VGC82_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0425334.1"
                     /translation="MPETTGRLDRGNGTELAWVRLDGRGPTIVFLPGFRSDMTGDKAT
                     ALASFCSDRGVAMLRFDYSGHGASSGDFLDGTIGAWTEDALAAIDALTTGKLILVGSS
                     MGGWIALLMALARPERVAALVGI"
     gene            472..1611
                     /locus_tag="VGC82_18595"
     CDS             472..1611
                     /locus_tag="VGC82_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0425335.1"
                     /translation="MPLSPDPPVVLQVLPSLITGGVERGTVEIARAIAEAEATALVAS
                     AGGPLVRQIERAGGTHIALPLTTKSPFGIWRNAGALEEVIRQRKVSLVHARSRAPAWS
                     AWIASQRTGVPFVTTFHGTYSETFPLKRQYNAVMARGRIVIAASHYIAGLIMDRHHLD
                     ASRIRVIPRGVDLTVFDPDNVSGTRVAKLAAEWRLPDGVRTVVLPGRLTSWKGHFVLL
                     DAMARLNRQDVMCVLVGSDQGRHDYAAALERQATRLGIASRLRLVGQCDDMPAALCLS
                     DVVVHASTRPEAFGRVIIEAQAMGRPVIAADLGGPVETVRHGETGWLVPPGDPAALAA
                     AIEVALDLNPAERLALGHRARSSVPTIRAMQDATLDVYKTVLAAG"
     gene            1608..2183
                     /locus_tag="VGC82_18600"
     CDS             1608..2183
                     /locus_tag="VGC82_18600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEY0425336.1"
                     /translation="MIELATEKQKMLAGLIYAASDPELTAEREAAAAWMARYNGSLAV
                     PATERFLLLKQRLAEVGDGAEIRPPFHCDYGGNINVGRGVFLNFNCIILDVVSVRIGA
                     GTQIGPAVQIYTADHPRDPAERRAGKEFGRPVTIGENVWIGGGAIILPGVTIGDDAII
                     GAGSVVTRDIPPGATARGNPARLAKSAPAFP"
     gene            complement(2242..>2424)
                     /locus_tag="VGC82_18605"
     CDS             complement(2242..>2424)
                     /locus_tag="VGC82_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425337.1"
                     /translation="LAVSADGFAMVGRQIAALGLPTVLIQEGGYLIERLGANLTAFFT
                     GFGIFRTGRGDPADNG"
BASE COUNT          427 a          789 c          796 g          412 t
ORIGIN      
        1 cgatgcccac cagagctgcg acccgttcgg ggcgggccaa tgccatcagc agggcgatcc
       61 agccccccat ggacgacccg accaggatca gcttgccagt cgtcagcgcg tcgattgccg
      121 ccagcgcatc ttcggtccag gcgccaatcg tgccatccag gaaatctccg ctgctggcgc
      181 cgtgtccgga gtagtcgaag cgcagcatcg ccactccacg atccgagcag aacgacgcga
      241 gagcagttgc cttgtcgccc gtcatgtcgg agcggaaacc cggcaagaac acgatggtgg
      301 ggccgcgtcc gtcgaggcgg acccaggcga gttcggtgcc attgcctcga tcgagccgtc
      361 cggtggtttc aggcatgaaa tcgatcctgc gcgcctacag catcatgaga acggatgaat
      421 cccaacataa caacatcgtc cgcgcggtga cagccgtggt agggtgacgc aatgcctttg
      481 tcacccgatc cgcctgtcgt gctgcaggtc cttccgtcgc tgataaccgg tggcgtggag
      541 cgtggcaccg tcgaaatcgc ccgagccatc gccgaagccg aggccactgc cctggttgcc
      601 agcgccggtg gtccgctggt gcgccagatc gagcgcgccg ggggcaccca tatcgccctg
      661 ccgttgacca ccaagtcgcc gttcggcatc tggcgcaatg ccggcgcact ggaagaagtc
      721 atccgccagc gcaaagtgtc gctggtgcac gccaggtccc gagccccggc gtggtccgcc
      781 tggatcgcga gccagcgcac cggcgtgccg ttcgtgacca cgtttcacgg gacctacagc
      841 gagacctttc cgctcaagcg gcagtacaac gccgtcatgg cgcgcggccg catcgtcatc
      901 gcggcgagcc actacatcgc tggcttgata atggatcggc accacctcga tgcttcccgc
      961 atccgggtga tcccgcgcgg tgtggacctc acggtattcg atccggacaa cgtatccggg
     1021 acccgggtcg ccaagctagc ggccgagtgg cggctgcccg acggcgtgcg tacggtggtg
     1081 ctcccagggc ggctgacgtc ctggaaagga catttcgtcc tgctcgacgc gatggcgcgg
     1141 ctgaaccggc aagacgtgat gtgcgtgctg gtcggatctg atcaaggccg ccacgattac
     1201 gccgcggcgc tggagcgtca ggcaacacgg ctcggtatcg cgagccggct gcggctggtc
     1261 gggcaatgcg atgacatgcc cgccgcgttg tgcctttcgg acgtggtcgt ccacgcctca
     1321 accaggcccg aggcttttgg gcgcgtgatc atcgaagccc aggcgatggg ccggccggtg
     1381 atcgctgcgg atctcggcgg cccggtggag acggtgcggc acggcgaaac cggctggctg
     1441 gtaccgccgg gtgatccagc agcgctggcc gctgccattg aggtcgcact ggacctgaac
     1501 ccggcggagc gtcttgcgct cggccaccgc gcgaggtcat ccgtgccgac aatacgggcg
     1561 atgcaagacg ctacgctgga tgtttacaag accgtgctgg cagcgggata atcgaattgg
     1621 cgactgaaaa gcagaagatg ctggcaggcc ttatatatgc cgccagcgat ccggaactga
     1681 ccgccgagcg ggaggccgcc gcagcgtgga tggcgcggta caatggttca ctcgccgtgc
     1741 cggccaccga gcggtttctt ttgctgaagc agcggcttgc cgaggtcggt gacggtgccg
     1801 agatccggcc gcctttccac tgcgactatg gcggcaacat aaatgtcggg cggggcgtat
     1861 tcctcaattt caactgtata attctcgatg tagtgtccgt tcggatcggt gccggcacgc
     1921 agatcggtcc ggcggtgcag atctataccg ccgaccatcc tcgggacccg gccgagcgcc
     1981 gggcaggcaa agaattcggc cgccctgtca cgatcgggga gaatgtgtgg atcggcggtg
     2041 gcgccatcat cctgccgggc gtgaccatcg gggatgatgc gatcatcggc gccggcagcg
     2101 tcgttacccg cgacattccg cctggagcga ccgcccgcgg caatcccgca cggcttgcca
     2161 agtcggcgcc cgcatttccc tgacgatgtt cctgtggaac gcgaagtggc ctcgcgaacc
     2221 acggaccaca ctcggcagct ttcaaccatt gtccgctgga tcgccgcgtc cggtgcgaaa
     2281 gatcccgaac ccggtgaaaa acgcggtcag attggcgccg aggcgttcaa tcaggtagcc
     2341 gccttcctgg atcagcaccg tcggcagacc cagggcggct atctgacggc cgaccatcgc
     2401 gaatccatcc gcgctgacag cgag
//
LOCUS       DASWQN010001088         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_633334, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001088 DASWQN010000000
VERSION     DASWQN010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633334"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VGC82_18610"
     CDS             <1..555
                     /locus_tag="VGC82_18610"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0425338.1"
                     /translation="GMNTGRAPERLGNASPAYVPTADSFPVGGGESLIAALTERQWQG
                     ICTAINRPDLPTDPRFTTTNARRANAPALRAVLIEAFASADAAEWEKRLSAAGVPAAP
                     ILTLQDALKHPQLAHRNIVSRLPGALGIDREVALSGSGFTASRDGPEVVALPPAKGQH
                     TADVLREAGYSINEIEALRAERVV"
     gene            681..1691
                     /locus_tag="VGC82_18615"
     CDS             681..1691
                     /locus_tag="VGC82_18615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEY0425339.1"
                     /translation="MRAVRCHDWMPYQNLPLEDVPPPVLGARQVRIGVHYAGVSFATS
                     LVTTGHYQRRPPRPFTPGTEIAGEVLETAPGLTQIAVGDRVCAVVDWGGHAEEVVIVP
                     ATVYKLPASMPLYLAPQFPTSYATAFAALDWRARLQPNETLLVHGAAGAVGLAAVELG
                     KAMGARVIATASTLEKLAVAQAHGADGTVLFPSGGALDAIKALAPNGADVVFDPVGGD
                     AFDLSLRAVAQCGRILVIGFASGRIQQIPANILLVKNVAVLGFNYGKYIGWGLVDERH
                     QHEPMVRAAFERMFLWFEQGKLHPTTSHRFPLSDYAAAMDAVLARLAVGKVVLEMPRA
                     QH"
BASE COUNT          388 a          675 c          708 g          398 t
ORIGIN      
        1 gggatgaata ccggccgcgc accggagcgt ttgggcaacg catcacccgc ctatgtaccc
       61 acagcagatt cgtttccggt cggtggcggc gagagcctga tcgcggcact gacggagcgg
      121 cagtggcagg gcatctgcac cgccatcaat cgtccggacc tgccgaccga tccacgcttc
      181 accacgacga acgcgcgccg cgccaacgct ccggcgctac gggcggtgct gatcgaggcg
      241 ttcgcatccg ccgacgcggc cgaatgggag aagcgcctat ccgccgcggg cgtgccggca
      301 gcaccgatcc tcaccctgca ggatgcgctg aagcacccgc aactcgccca ccgcaacatc
      361 gtttcgcggc tgccgggcgc gttgggtatc gaccgggaag tcgcgttgag cggctccgga
      421 ttcaccgcca gccgggatgg gccagaggtg gtggcgctac cgccggcgaa gggacagcat
      481 accgcggacg tgctgcggga ggcgggctat tcgatcaacg agatcgaggc gcttcgggcg
      541 gagcgggtgg tgtaaagacc tgtgatccac ggcaacctgc aacagggcgt gatccgcgga
      601 ggtggtatca tcggagcggt ttatcgcccc ctgaagacaa tgtctccaat gatcacgaga
      661 taacaagacg ggagagcaat atgcgcgcgg tgcgctgcca tgactggatg ccctaccaga
      721 atcttccact tgaagacgtg ccgccaccgg tgctcggagc ccggcaggtg cgcatcggcg
      781 tgcattatgc cggcgtttcg ttcgcgacat ccctggttac gactggccac tatcaacgcc
      841 gcccaccgcg tccgttcacc cccggcaccg aaatcgccgg cgaggttttg gaaacagctc
      901 ccggcctcac ccagattgca gtcggcgacc gggtctgcgc ggtcgtcgac tggggcggtc
      961 atgccgagga agtggtcatc gttccagcca ctgtctacaa gctgcctgcc tcgatgccgc
     1021 tttaccttgc gccgcagttt ccgacgtctt acgccacggc gtttgcggcg ctggactggc
     1081 gggcccggtt gcagccgaac gagaccctgc tggtgcacgg cgcggccggc gcagtcgggc
     1141 tggccgcagt cgaacttggc aaagcgatgg gcgcaagggt catcgccact gcctctacac
     1201 tggaaaagct tgcggttgcg caggcccacg gcgcggatgg cacggttcta tttccatccg
     1261 gcggcgcatt ggacgcaatc aaggcgctgg cgccgaacgg ggcggacgta gtgttcgacc
     1321 cagtcggcgg cgatgcgttc gatctgtcgc tgcgcgcggt ggctcaatgc gggcggatcc
     1381 tggtgatcgg ctttgccagt ggccggatcc agcagatccc agcaaacatc ttgctggtga
     1441 agaatgtcgc ggtcctcggt ttcaactacg ggaagtacat tggctggggc ctcgttgatg
     1501 aacgccacca gcatgagccg atggtgcgcg ctgccttcga aaggatgttc ctctggttcg
     1561 agcaaggcaa gctgcacccg acgacatcgc accggttccc tttgagcgac tatgcggcag
     1621 cgatggatgc ggtgctcgcg cgtctggcag tcggcaaggt tgtgttggag atgccgcgcg
     1681 cgcagcattg atcaagatcg gccgctcacc gacttggcct gtcgccagcc accagttctt
     1741 ccacggcgac cagttcttca cggcgaccag ttcttcacgg cgaccagtag cggggcatca
     1801 tgtggtttgc cgcaacaacg gcgtcgtcgt caggcgcggc ggcggtcgcg ccggtatgac
     1861 aacaccatta cgctgtcgcc accgttggtt attgcgatat ttgaacgcaa cggtatcctt
     1921 ggaattctgg accaagcgct cggaacgctg gacctgggct gaatttaccg cggcatcgcg
     1981 gcggcggtca tgcacctttc gggcgcggcc ccacacggca cctgagtccg ggtagcagca
     2041 aggatctttt tctacgccga tgtcctcagc tatcgagtta aacccaatac cgttcactta
     2101 agcacccgga tagctgctgc gtagtcgata cggatgattg gatgcggcga gcggggacgc
     2161 aggtgatag
//
LOCUS       DASWQN010001089         4169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_763879, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001089 DASWQN010000000
VERSION     DASWQN010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763879"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VGC82_18620"
     CDS             <1..801
                     /locus_tag="VGC82_18620"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0425340.1"
                     /translation="PMLVKNLPKMIRYLKDRGTYVLFNTNGTLLNPKKFQEIIDTGLD
                     ELRVSLDAADRKSYAAIRGKDFFPRIVRDVGKFTAYQQQMGVSHPRVSLWLTGLKETV
                     EQLPAFVRLSASMGVTEVHLQRLVFDEAGFGKARAESSLFEQTRDDEQSAIDAAQAVG
                     AELGVTLDASGATEPGLSLKRAADDKPWATCRRPWSLMYFTAHGRALPCCIAPFSARG
                     YENYTLGDATQDTLRDIWNSPAYRGFRTSLLGETPPMPCQGCGLRWSL"
     gene            824..1585
                     /locus_tag="VGC82_18625"
     CDS             824..1585
                     /locus_tag="VGC82_18625"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0425341.1"
                     /translation="MEPDEMQPDEMHPGPLLTTEFAGTALSVVIPVFNEAESIASVIE
                     EIPGDLVGEVIVADGGSTDGTQDIARVAGARVLDAGRGYGRACQAGAQAAMGAVVVFL
                     DGDGADRGDLLRLIAEPVLAGTHDLVLASRTSGKRDPGSMLWHQVLAGRLAGWGIGAL
                     YGVRYSDMCAFRAIRRDALIRLDMQEMTYGWNIEMQMKAARGGLRIKEIPMPYRCRMG
                     GTSKVAGSLPGTLRAGGKIVSTFARVASQRSAGAG"
     gene            complement(1773..4121)
                     /locus_tag="VGC82_18630"
     CDS             complement(1773..4121)
                     /locus_tag="VGC82_18630"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0425342.1"
                     /translation="MTQPDGEPFHIEEATIDDLHQAIRTGRTTCVGVVQQYLDRVRAY
                     NGVSSMLVTQDGEPVPEATGVVRGKAPIRFPTQTLKASAILPDLDKYQGPPLEFGRME
                     ATASDPSVQQQFGMIAGIPNAGQVNALGTINIRSERSVTCRGDFDRHPLQGPLPPGAP
                     PVCEMFRQQPDALERAAALDAAFGHNPDLAAMPMYGVVVSFKDPFDTKDMRSTGGGDA
                     AYDIDFPARDHILVEQLRAKGAIVFAKAVNTEYNGRAGDPGGRNYPDKVLPSTLGYQR
                     STWAGNPSNPYDTTRAASLGSSSGSAVSVSTNLVMISLGEETRASCRGPSNHNSVALL
                     LPHKAMIGFDGGAIGADIYCDRSGIHARRIKDAAKVLDALKDPVHGYYDPRDSYTTVP
                     RSSVLPTQYASHATMPGAPGTLAGMRIGVIRESMLNPGTKKHVEPITTAAASEIKTIL
                     AGKLGATLVESSDPVWTPDPDIAPMRTDFRTALARLVPVFMPELLFRLGADGQPVFKA
                     FAAAIQPTQFMPGKVFGTGTMAPIDYLVEMAEGRIAPPANLDIATIQNQELAMSFRFH
                     IPQYLTRRAADWRNLGFTETVTDFAKLNARSRFWGDDQRAAFRNWEEVTDPRNALGGR
                     QGVDERIMLRELLRRVDMMVILENSLDALVRLHTPLPPGKIGGAIEPGTPGNLRMESF
                     YGPNAGLTEVLIPAGYVKTVYDPVFRLSADRRHYVPTLSDEPTTVADPGMPFSLVFRA
                     EPGREDVLLKIASAYEAASMRRVPPPAFGRLTDSGFAIFPGG"
BASE COUNT          717 a         1240 c         1419 g          793 t
ORIGIN      
        1 ccgatgctgg tcaagaacct gccgaagatg atccgttacc tgaaggaccg cggcacatac
       61 gtgttgttca acaccaacgg gacgttgctg aacccaaaga agtttcagga aatcatcgac
      121 acggggctgg acgaacttcg cgtgtcgctg gatgcggccg accgcaagag ctacgcggcc
      181 atacggggca aggatttctt cccgcgcatc gttcgcgatg tggggaaatt taccgcctac
      241 cagcagcaga tgggggtttc gcatccccgt gtttcattgt ggctgacggg gttgaaagag
      301 acggtggagc agttgccggc gttcgtgcgc ctgtcggcct ccatgggcgt gacggaagtc
      361 catctgcagc gcctggtgtt cgacgaggcc ggattcggca aggcacgcgc ggagtcttcg
      421 ctgttcgagc agacgcggga cgacgagcaa tcggcgatcg atgcggcgca ggcggtcggc
      481 gcggaactag gggtgacgct ggatgcatcc ggtgccacgg agccagggtt gagcctgaag
      541 cgggcggcgg acgacaagcc atgggcgacg tgccggcggc cttggtcgct gatgtatttc
      601 accgcgcatg gccgagccct gccctgctgc attgcgccgt tctcggctcg gggatacgag
      661 aattacacgc tcggggacgc gacgcaggat acgttgcgcg acatctggaa cagccccgct
      721 taccgcggct tccgcacgtc gctgctgggg gagactccgc cgatgccctg ccagggatgt
      781 gggctgcgct ggagtttgtg aaacggcgca ggagcctgat gggatggagc ccgatgagat
      841 gcagcccgat gagatgcatc ctgggccact tctgaccacg gaattcgcgg ggacggcgct
      901 gagtgtcgtc attcctgttt tcaatgaagc cgaatctatt gcttcggtca tcgaggaaat
      961 tcccggcgac ctagttggcg aggtaattgt tgccgacggc ggcagtaccg acggcacaca
     1021 agatatcgcg cgtgtggccg gggctcgcgt tctcgatgcc gggcgcggtt atggacgggc
     1081 gtgccaggcg ggcgcacagg ctgccatggg cgccgttgtc gtgtttttgg atggcgatgg
     1141 cgccgatcgc ggcgacctgt tgcggctgat cgccgaaccg gtcctcgcgg ggacgcatga
     1201 tcttgtcctg gcgtcccgca ccagcggcaa acgcgatccg ggatcgatgc tttggcatca
     1261 agtgctggcg ggacgacttg ccggatgggg catcggagct ttgtacggcg tccgctacag
     1321 tgacatgtgc gctttccggg caatccggcg ggacgcgctg atccggctgg atatgcagga
     1381 aatgacctat ggctggaata tcgagatgca aatgaaggcc gcgcgtggcg gcctgcgcat
     1441 caaggagatt cccatgccgt accggtgccg catgggcgga acgtcgaagg tcgctgggtc
     1501 tttgccgggc acgctgcggg ctggcggcaa gatcgtgtcc accttcgctc gggtggcctc
     1561 gcagcggtcc gccggggctg gctaactgta acgaccaatc gcggcgtctg ccagagcgct
     1621 ttcacgctgg ttgaaaaagg tgcgagtcgg tttgccgcct gacgcctaga tgtcatggcc
     1681 gtgccaagcg aggtcaccca ctttcttgca gccataagag aactttgatt ccggccatcg
     1741 tgaagtcatt ataatcagtc gtcgttcagg catcatcctc cggggaatat ggcgaagcca
     1801 ctgtccgtca gccggccgaa cgccggtggc ggcacacgcc gcatcgaggc cgcttcgtat
     1861 gccgacgcga tcttcaacag cacgtcctca cggccaggtt cggcacggaa aaccaacgag
     1921 aacggcatgc cgggatctgc gacagtcgtc ggttcgtcgg acagcgtcgg cacatagtgc
     1981 cggcggtcgg cactgagcct gaacaccggg tcatagaccg tcttcacgta gccggcggga
     2041 atcagcacct cggtcagccc cgcattcggg ccgtagaagc tttccatccg caggttcccc
     2101 ggagtgccgg gttcgatggc gccgccaatc ttaccgggcg gcaggggcgt gtgcagccgc
     2161 accagcgcgt ccaggctgtt ctccaggatc accatcatat cgacgcggcg cagcagttca
     2221 cgcagcatga tccgttcatc gacaccctgg cggccaccaa gggcgttgcg cgggtcggtc
     2281 acttcctccc aattcctgaa ggcggcccgc tggtcgtcac cccagaacct ggagcgggcg
     2341 ttcagttttg cgaaatcggt cacggtttcg gtaaaaccaa ggttccgcca gtcggcggcg
     2401 cggcgggtca ggtactgcgg gatatgaaaa cggaacgaca tcgccagttc ctggttctgg
     2461 atcgtggcga tgtccaggtt tgccggcggc gcgatgcggc cctccgccat ctctaccagg
     2521 tagtcgatcg gcgccatcgt tccggtgccg aagaccttgc ctggcatgaa ctgcgtcggc
     2581 tggatcgcgg cggcgaacgc cttaaacact ggttgcccgt ccgcgcccag gcggaacagc
     2641 agctctggca tgaataccgg aaccaaccga gccagcgcgg tgcggaaatc cgttcgcatc
     2701 ggcgcgatat ccggatcggg cgtccacacc gggtcggatg attccaccaa ggtggcgccg
     2761 agttttccgg ccaggatcgt tttgatctcg ctcgcggccg cggtggtgat cggttcgaca
     2821 tgcttcttcg tgccggggtt gagcatcgac tcgcggatga cgccgatccg catgccggcc
     2881 agcgtgccgg gcgcacctgg catcgtggcg tgcgacgcat actgcgtggg caggacggac
     2941 gatctcggca cggtcgtgta ggaatcgcgc gggtcgtagt agccgtgcac cggatccttc
     3001 agcgcatcca gcaccttcgc ggcgtcctta atacgtcgag cgtggatacc gctgcggtcg
     3061 cagtagatgt ccgcgccgat cgccccgcca tcgaacccga tcattgcctt gtgcggcagc
     3121 agcagcgcca ccgaattgtg gttcgaagga cctcggcagg atgcccgtgt ttcctcgccc
     3181 aggctgatca tcaccaggtt ggtgctgacc gacacagccg aaccggagct ggaaccaagc
     3241 gaagcggcgc gggtggtgtc ataggggttg gacggattgc cggcccaggt gctgcgctga
     3301 tagcccaggg tcgagggcag caccttgtcc ggataattgc gcccgccggg gtccccggcc
     3361 cggccgttgt attcggtatt caccgccttg gcgaagacga tcgctccctt ggctcgcagt
     3421 tgctcgacca ggatgtggtc ccgggctgga aagtcgatgt cgtatgcggc atcgccgcca
     3481 ccggtggagc gcatgtcctt cgtgtcgaac ggatccttga aggaaaccac gacgccgtac
     3541 atcggcatcg cggcaaggtc cgggttatgg ccgaaagcgg cgtccagggc ggcggcgcgt
     3601 tccagcgcgt cgggctgctg gcggaacatc tcgcagaccg gtggagcgcc tggcggcagt
     3661 ggaccttgca gcggatggcg gtcgaaatcg ccgcggcagg tgacggaccg ttcgctacgg
     3721 atgttgatgg tgccgagggc gttgacctgg ccggcgttag ggatgccggc gatcatgccg
     3781 aattgctgct gcacggacgg gtcggatgcc gtcgcctcca tgcggccaaa ctccaacggc
     3841 ggaccctggt atttgtcgag gtcgggcagg atggcggacg ctttcaaggt ctgcgtcgga
     3901 aaacggatcg gcgctttgcc gcgaacgacg cctgtggctt ccgggaccgg ttcgccgtct
     3961 tgcgtgacca gcatgctgct gacgccgttg taggcccgaa cgcggtcgag atactgctga
     4021 acgacaccga cgcaggtggt gcggccggtg cggatcgcct gatgcagatc gtcgatagtc
     4081 gcctcctcga tgtggaatgg ttcgccgtct ggctgcgtca tcgtgcaatc cttctgctat
     4141 ccggttaggg cttgatcacg ctggacgga
//
LOCUS       DASWQN010001090         4259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_897900, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001090 DASWQN010000000
VERSION     DASWQN010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897900"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VGC82_18635"
     CDS             complement(<1..621)
                     /locus_tag="VGC82_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425343.1"
                     /translation="MRRFSFIRPGGGRALDLLNFFVADVQTGFGPFVAVYLTTHKWTQ
                     VEIGYALTLGTMTSMVSQLPAGILVDSMRNKRWAASGALIAIIVAALLLAIQPEQLPV
                     LIAQILHGFASCVITPAIAAISLHLVGHAALGERLGRNARYASVGNGLAAAVMGATGA
                     YFSSRFVFLLTAALCVPALVALFSIGTGHHGRGQTTSRVRDWSGLKR"
     gene            complement(625..1116)
                     /locus_tag="VGC82_18640"
     CDS             complement(625..1116)
                     /locus_tag="VGC82_18640"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY0425344.1"
                     /translation="MRRSVLVGGMLTALFAGPVAQADEAPPPAVQSVAPEDAEAILGE
                     RVTDQDGKDLGRLVDVLVDANGQPQAAIIDFGGFMGVGNRKVAVHWSGLQFRPADAKH
                     KVTLEMTPDQIKAAPEFRNPNKAAPVVIPAELAPPAPPTPTAASPTAASPTAPAAPLQ
                     ASP"
     gene            complement(1194..2111)
                     /locus_tag="VGC82_18645"
     CDS             complement(1194..2111)
                     /locus_tag="VGC82_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0425345.1"
                     /translation="MFRSIWQSAWVLLAAANLFWAGNIVLGRALSGHFPPVALAYWRW
                     TGAFIIAVGFAWPRLKADLPVMLRHWKMMLALSATGIACYNTMSYIGLTSTTALNVLL
                     LQSATPLIIIVWAYLVFRETPTPRQALGVLVSLAGVGVIGGHGSLEVLAHFRLNDGDL
                     WVLAALTIYGFYCVMLRDRPPVHPLSFLVAAMGIGSLMMLPFMLWEFASGARVSGGVA
                     PYLAIAYIAVLPSFVAYLCFNRGIELIGPGPAGQSMHLMPLFGSVLAVLFLHEQFQPY
                     HAAGIALIAAGILLASFSSGRQRAVRDST"
     gene            2178..3494
                     /locus_tag="VGC82_18650"
     CDS             2178..3494
                     /locus_tag="VGC82_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400200.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEY0425346.1"
                     /translation="MQRIVLKSETDWEGWRQATRALVLTGTEPQAVTWSVGKQASALA
                     EESGSFHVPRALVSLASLAIQARDQERFGLLYGLVWRIHNGEKVLEDAADPDLSLVRR
                     MALSVRADAHRMRTNIRFLPVPEPGGTRFLGWFEPAHFVLPANARLLARRYPNLSLSI
                     VTPDGAAHWDGSSLLFGTGLRHAADDEALQAWWEAHRDRLLEQATADVSVPEAEEVDE
                     APRSPDRPALGPVVLHTKPDAALLRSGLDAAACHRCPLYEPATQTVFGEGPADAKAMF
                     VGEQPGDQEDTIGRPFVGPAGQMMDRAMEEAGIDRRTAYVTNAVKHFKFTQRGKRRIH
                     QTPEVPEIQACNFWLDVERGHVRPNLLVLMGGSAARAVLGRAVTISRERGRPIRMADG
                     QTVFVTVHPSYLLRIPDAAAKAREYAAFVRDLQAVRELVAADQAGA"
     gene            complement(3491..3874)
                     /locus_tag="VGC82_18655"
     CDS             complement(3491..3874)
                     /locus_tag="VGC82_18655"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0425347.1"
                     /translation="MIKFNWEHIHLRSPDPEATAEWYKDKLDAEVIKTPQADGSVRVD
                     LNLAGQKILIAKAMPGKAAAAPASPYLGLDHFGLTVPDIKAAVAALKAKGVTFTMDVT
                     TNRPGTQIAFFTAPENVSIELIQRG"
     gene            4059..4148
                     /locus_tag="VGC82_18660"
     tRNA            4059..4148
                     /locus_tag="VGC82_18660"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4093..4095,aa:Ser,seq:cga)
BASE COUNT          814 a         1363 c         1359 g          723 t
ORIGIN      
        1 gcgcttcagg cccgaccaat cccgcacccg gctggtggtt tgccctcggc cgtggtggcc
       61 cgtgccgatc gaaaacagtg ccaccaatgc gggcacgcat agggcggcgg tcagcagaaa
      121 aacgaagcgc gacgagaaat aggcacccgt cgcacccatc accgcggcgg ccaagccgtt
      181 gccgaccgaa gcatagcgcg cgtttcgccc cagccgctcc cctaaggcag catggccgac
      241 caggtgtaag ctgatggcgg cgatggccgg cgtgatcacg caactggcga agccatggag
      301 gatctgcgcg atcagcaccg gcagttgctc cggctggatt gccagaagca gtgcggccac
      361 gatgatggcg atcagcgccc ccgatgccgc ccaccgcttg ttgcgcatcg aatcgacaag
      421 gatgccagcc ggaagctggc tgaccatcga cgtcatggtg cctagcgtca gggcatagcc
      481 aatctcgacc tgcgtccatt tatgcgtcgt gagatagacc gcgacgaacg gtccaaaccc
      541 cgtttgcaca tccgccacga agaaattcaa cagatcgagc gctcggccgc cgccgggccg
      601 gataaaacta aatcgccgca tgattcaggg cgacgcctgc aaaggggctg ccggcgcggt
      661 aggtgacgcg gcagtaggtg acgcggcagt gggggttggc ggggcaggtg gggcgagctc
      721 cgccgggatg acgacgggcg ccgccttatt gggattgcgg aactcgggcg cggccttgat
      781 ctgatccgga gtcatttcca aggtgacctt atgtttggcg tctgctggcc ggaattgcag
      841 cccactccaa tgcacggcga ccttgcgatt gccgacgccc atgaacccgc caaagtcgat
      901 gatcgcggcc tgaggctggc cgttcgcgtc caccagcaca tcgaccaggc gaccgagatc
      961 cttgccatcc tggtcggtga cacgttcccc aagaatggcc tcggcatcct cgggcgccac
     1021 ggactgaacg gcgggcggtg gggcctcgtc ggcttgggcg accgggcccg caaacagggc
     1081 ggtcagcatt ccgccaacca aaaccgatcg tcgcatcagt cctccagttc gccaactagt
     1141 cctcagccca tggactatac cgatcagcca accggttcaa cggagttatc acattatgtt
     1201 gagtcgcgga ccgcccgctg tcgcccggag ctgaaggaag ccagcaagat accagccgcg
     1261 atcaacgcta tcccggcggc gtggtaaggc tgaaattgct cgtgcaggaa cagcaccgcc
     1321 aggacgctgc cgaacagcgg catcagatgc atcgattgcc ccgccgggcc gggaccgatc
     1381 agttcaatgc cgcgattgaa gcaaaggtag gcgacaaacg acggcaacac ggcgatgtag
     1441 gcgatggcga ggtagggcgc cacgccgcca ctgacccgtg caccggaggc aaactcccac
     1501 agcatgaacg gcaacatcat gagggacccg atccccatgg cggcgaccag gaagcttaac
     1561 ggatggaccg gcggccgatc gcgcagcatg acgcaataaa acccgtagat cgtcagcgcc
     1621 gccagaaccc atagatcgcc gtcgttcagc cggaaatgtg ccagtacctc cagcgacccg
     1681 tgcccgccga tcactccaac tccggccaga gacaccagca cgcccagcgc ctgccgcggc
     1741 gttggcgttt cgcggaacac cagatacgcc cagacgatga taatcaacgg ggtcgcggac
     1801 tgcagcagca gcacgttcaa ggccgttgtg ctggtcaggc cgatatacga catcgtgttg
     1861 tagcaagcga tgccggttgc cgatagcgcc agcatcatct tccagtgccg cagcatcacg
     1921 ggtagatcgg ccttcagcct gggccatgcg aagccgaccg cgatgatgaa agccccggtc
     1981 cagcgccaat aggccaaagc caccggtgga aaatgcccgg acaatgcgcg cccgagcacg
     2041 atgttgcccg cccagaagag attggccgcc gccaacagaa cccaggccga ttgccatatt
     2101 gacctaaaca tctgccagcc aacatagccc ggacggcagc cttggaaacc cgtgcatccc
     2161 ggagtttgct tgttcccatg caacgcatcg tcctgaagag cgagaccgac tgggagggct
     2221 ggcggcaggc gacacgcgcc ctcgtgctga ccggcaccga accccaggcg gtgacctggt
     2281 ctgtcggcaa gcaagccagc gcgcttgccg aggagtccgg cagcttccat gtgccgcggg
     2341 cgctggtgtc gctcgcctcg ctggcgattc aggcgcgcga ccaggagcgg ttcggcctgc
     2401 tgtacgggtt ggtctggcgg atccataacg gtgaaaaggt gctggaggac gccgccgacc
     2461 cggatctgtc gctggtgcgc cggatggcct tgtccgtgcg tgccgacgcc caccgtatgc
     2521 gtacaaacat tcggttcctg ccggttcccg aaccgggcgg gacgcgcttc ctcggctggt
     2581 tcgagccggc gcacttcgtg ctgccggcca atgcgcggtt gctggcccgc cgttacccga
     2641 acctgtcgct gtcgatcgtg acgccggatg gcgccgccca ttgggatgga tcgtcgctgt
     2701 tgttcggcac cggcctgcgt catgcggcgg atgacgaggc cctgcaggca tggtgggaag
     2761 cgcatcgaga ccgactgctg gagcaggcca ctgccgatgt ctcggtcccg gaagctgaag
     2821 aagtggacga ggcgcctcgt tcgcccgacc gcccggcact gggtcctgtc gtcctgcaca
     2881 ccaagcccga tgcggccttg ctacgctcgg gcctggacgc cgcggcctgc catcgttgcc
     2941 cgctttacga acctgcgaca cagacggtgt ttggcgaagg gccggccgat gcgaaggcga
     3001 tgttcgttgg tgaacagccg ggcgatcagg aggataccat cggccgcccg ttcgtcggcc
     3061 ccgccggcca gatgatggac cgcgcgatgg aggaagccgg catcgaccgg cgcaccgcct
     3121 atgtcaccaa cgctgtcaaa cacttcaagt tcacccagcg cgggaagcgg cggatccacc
     3181 agactccgga ggtgccggaa attcaggcct gcaatttctg gctggatgtg gagcgggggc
     3241 atgtccgtcc caacctgctg gttctgatgg gcgggtcggc tgcgcgggct gtattgggac
     3301 gcgcggtgac gatcagccgg gagcgcgggc ggccgatccg catggcggac ggacaaacgg
     3361 tctttgtcac cgttcacccg tcctatctgc tccgcattcc cgacgccgcc gccaaggctc
     3421 gggagtatgc agccttcgtg cgggatctgc aggcggtacg cgaacttgtg gcggcagatc
     3481 aggctggcgc ctaaccccgc tggatgagct cgatcgagac gttctccggc gcggtgaaga
     3541 atgcgatctg cgtgccaggc cggttggttg tcacgtccat cgtgaacgtc acaccctttg
     3601 ccttcaaagc agcgactgct gccttgatgt cgggcaccgt cagtccgaag tggtcgagcc
     3661 cgagatacgg cgatgccggc gcggcggcag ccttgcccgg catggctttg gcaatgagta
     3721 ttttctgtcc cgcgagattg aggtcaacgc gcaccgaccc atcggcctgc ggggttttga
     3781 tcacctcggc gtccaatttg tccttgtacc attccgcggt cgcttccgga tcaggcgagc
     3841 gcagatggat gtgttcccag ttgaacttga tcatgtttcc tccagatggt cttcggagcc
     3901 attaactcac catagccgga cctggacaag ggtctggacc cgatgtgtcg acccgatgtt
     3961 tggcgttgac ttgtcaggat gtgccggacg attgccgaag cttgcgttgg ggaggagtac
     4021 cggctaggtg acgcctcaga tgctcggttc acgctaccgg agaggtgcca gagcggtcga
     4081 tcggggcggt ctcgaaaacc gttgagcgcg caagcgttcc gagggttcga atccctccct
     4141 ctccgccaac tgacgcccca cggcgtcgcc ctacatccca agcctcccga aaaaaagcaa
     4201 tggcgtagcc gagtcaccgt ccccagaggt catcagtcgg ctccgagtat cccaatcaa
//
LOCUS       DASWQN010001091         4225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_238452, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001091 DASWQN010000000
VERSION     DASWQN010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238452"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VGC82_18665"
     CDS             <1..680
                     /locus_tag="VGC82_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OBAP family protein"
                     /protein_id="HEY0425348.1"
                     /translation="PEQPPPGAPLSTRSHVLDTGADALQYKRPLDAMSEYLNGFHFYA
                     DDLGRQVEANHYCTHLTEDFHQCVIYSSNRRDAKLIGIEYIVSEKIFKSLPADEKKLW
                     HSHHYEVKSGELTAPGVPNVAEHAFMRDLVTTYGKTWHTWQIDRDPNLPIGIPQLMMG
                     FTRDGQINQKMLTDRDKVFGSSTEARRKNRDDIPMPEVAVGANAWQAGKVMQLKLEEV
                     AVRNLRI"
     gene            731..2023
                     /locus_tag="VGC82_18670"
     CDS             731..2023
                     /locus_tag="VGC82_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018980540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEY0425349.1"
                     /translation="MAKDRSHETTLGNHQLHPETLMLSYGYDPALSEGSAKPPVFLTS
                     TFVFRSAEEGRDYFNVVAGRTAVPEGGAGLVYSRFNHPNSEIVEDRLSIYEGAESAIL
                     FSSGMAAIATTLLAFARPTDAILHSQPLYGGTETLLARTFMEFGIAAVGFSDGTSAAA
                     MDHAAEQALKRGRVSVIMVETPSNPLNSLVDLALLSLAADKIEAAQGHRPVIVCDNTM
                     LGPVFQSPLKHGVDLVVYSLTKYVGGHSDLIAGAVLGARDKLRSIRLLRGAIGTQLDP
                     HSCWMLGRSLETLSLRMRAAARNAEGVAAFLADHPAVTTVHAFGSAAPDTAAGGIYER
                     QCTGSGSTFSIEIRGGQSEAFRVLNALRIFKLAVSLGGTESLASHPASTTHSGVPVDV
                     RRRIGVTDGLIRLSIGIEHADDLIADLAQALATVQGQP"
     gene            2085..3608
                     /locus_tag="VGC82_18675"
     CDS             2085..3608
                     /locus_tag="VGC82_18675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0425350.1"
                     /translation="MTDPGTAPIRSDEASSGRGSSGVIVDCAVLVGLAATLRFAWLGH
                     ASLWIDELFSVCWSQLDWHFLLREGARTETNPPTYYILLHAWMEVFGTTETAVRVPSA
                     LASTATVLVVYAIGRMMKDRRVAMLAALLLAVNPVAVASAPEARAYALSAFLDGVGLL
                     SIVGYVRRFGTIGTWSWGWLGAFVFSMIATASVHYTSLLFVAACFGAMGWRLVTTRPF
                     PVREALAWVAAGLLTALVLAKLLILAASLSESNNLVWIGPLTVWSVVSFFLELIVQLP
                     ETDAFLTVSWLVCAAFALVVIGARPRLRGDRQFGVLILIPVLYCALFIIAGWLRPMLL
                     ARVATWLVIPLCLILARAALSQSGRWRSYAACAVPLLTFLFSTGYYYRFHEKEDWRGA
                     IRLIATQPNCAGPLLVSEFNALGLYYYKIPAHRQAYVFLPDPRRRNSVEFSLSERLMH
                     LPELDPGAIIEFIETHPGTAVIMRWEYREVIPLDLQTLLAHAPTKARLDGGLTVACF"
     gene            complement(3665..>4225)
                     /locus_tag="VGC82_18680"
     CDS             complement(3665..>4225)
                     /locus_tag="VGC82_18680"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0425351.1"
                     /translation="EAMVAATVSCFGRIDVLVNNAGVAPAGPFAEAPIADWRRVMATD
                     VDGVLFCCRAALPHLLETRGCIVNTSSVSGLRGDWNMSFYNAAKGAVTNLTRALALEL
                     GERGVRVNAVNPSFTITDMTKDMEQDSALMERFKERIPLGRPAQPDDIAGVIAFLASD
                     DARFVTGVNLPVDGGLSASNGQPRQA"
BASE COUNT          809 a         1272 c         1265 g          879 t
ORIGIN      
        1 aaccggagca gccgcctccg ggcgctccac tgtcaactcg aagccatgtc ctggacacgg
       61 gtgcggacgc attgcagtac aagcgcccgc tcgacgcaat gagcgagtat ctaaatgggt
      121 ttcatttcta tgcagacgac ctagggcgcc aggttgaggc aaatcactac tgcacccatc
      181 ttaccgaaga ttttcatcaa tgtgttatct acagctccaa ccgccgggat gccaagctga
      241 ttggcataga atacatcgtc agcgagaaaa tcttcaaatc cctccccgcg gacgagaaaa
      301 aactttggca cagccatcat tacgaggtga agtccggcga gcttaccgct ccgggcgttc
      361 caaacgtggc agaacacgcg tttatgcggg atctggtcac cacctatgga aaaacctggc
      421 acacatggca gatcgaccgc gacccgaatc tcccaatagg cataccgcag ttgatgatgg
      481 gttttacccg cgacggccaa atcaatcaaa agatgctgac ggatcgggat aaggtattcg
      541 gctcgtcgac cgaggccagg cggaaaaacc gcgacgacat cccaatgccg gaagttgccg
      601 tgggcgctaa cgcctggcag gcaggcaagg tgatgcagct aaagctggag gaggtagcgg
      661 tacgaaatct ccgaatttga tcgggatcgg acccattatc ggctatagcg ggccggaatg
      721 gagtgaaaca atggccaaag accgcagtca cgaaaccact cttggcaatc atcaactcca
      781 cccggaaacg ttgatgctta gctatggcta tgatccagca ttgtcggaag gctccgccaa
      841 gccgccggta tttctgacct cgactttcgt gttccgttcg gcggaggaag ggcgagacta
      901 tttcaacgtg gtcgccggac gaacggccgt gccggaaggc ggcgccggac tggtctactc
      961 gcggttcaac catcccaaca gcgagatcgt cgaagacagg ctctcgatct acgagggcgc
     1021 cgagagcgcg atcctgttca gttccggtat ggcggcaatc gcaacgacct tgctcgcgtt
     1081 cgcccggcca acggatgcga tcctgcacag ccaaccgctc tatggcggca cagaaaccct
     1141 tctcgcccgc actttcatgg aattcggcat cgcagccgtc ggcttctccg atggcacgag
     1201 cgcagcagcg atggatcatg ccgccgaaca ggccttgaaa cggggccggg tcagcgtgat
     1261 catggttgaa acgccgtcaa atccgctcaa cagcctggtt gacctggcgc tgctatcgtt
     1321 agccgcggac aaaatcgagg cggctcaggg ccaccggcca gtcatcgtat gcgacaacac
     1381 gatgttgggg ccagtcttcc aaagtcccct caaacatggc gttgacctcg tcgtctattc
     1441 gttgacgaag tacgtcggtg gtcacagtga cctcatcgcc ggcgccgtgc tcggagccag
     1501 ggataaacta cgttcgatcc ggttgctgcg cggcgcgatc ggcacccagc tcgatcccca
     1561 ttcatgctgg atgctgggcc gctcgcttga aaccctgtcg ctgcgaatgc gcgcggcagc
     1621 ccgtaacgcg gaaggcgtag cagcgttctt ggcggaccat ccggcggtga cgaccgtaca
     1681 tgcgttcggc agcgccgctc cggataccgc cgcagggggt atttatgaac gtcaatgcac
     1741 cggctccggg tccacattca gcatcgagat tcgcggcggg cagtccgagg cattccgtgt
     1801 ccttaatgcg ctacggatat tcaaactcgc ggtcagcctg ggtggcactg aatccctggc
     1861 cagccatccc gcatccacga cgcattccgg cgttcctgtt gacgttcgtc gtcgcatcgg
     1921 cgtcacggac gggctaatcc gcctcagcat cggcattgag catgcagacg atctgatcgc
     1981 agacctggcg caggctctgg ccacggttca aggccagcct taagcttgat gaaccgggca
     2041 gcctgtgtcg gatgtatgcg tcgcatgacc gccaagaaga tcgcgtgacc gacccaggaa
     2101 cagctccgat ccgaagcgac gaggcaagct ccggtcgcgg gtcatccggc gttatcgtcg
     2161 attgcgcggt gttggtcgga ttggcggcaa ccctgcggtt tgcctggctg ggacatgcgt
     2221 cactgtggat cgacgagctg ttttccgttt gctggtcaca acttgattgg catttcctgc
     2281 tgcgggaggg cgcgcgcaca gaaaccaatc caccaaccta ctacatcctg ttgcacgcgt
     2341 ggatggaagt atttggaacc acggaaaccg ctgtccgggt tccgtccgcg ctggcttcga
     2401 cggcgacggt cctggtcgtc tatgcgatcg gccggatgat gaaggatcgg cgggtggcaa
     2461 tgcttgctgc cctgctcctg gcggtgaacc cggtagccgt cgcgtcggcg ccggaagcgc
     2521 gagcctatgc cctgtcggcc tttctggatg gcgtggggct gttgtcgatc gtgggctacg
     2581 tgcgccgctt tgggacaatc ggcacctggt catggggatg gttgggcgcc ttcgttttct
     2641 cgatgatagc aacagcatct gtacactata cttcgttgct gtttgtcgcg gcgtgcttcg
     2701 gtgcgatggg ttggcgactg gtaaccacgc gtccctttcc ggtccgcgaa gccctcgcct
     2761 gggtcgctgc aggtctgttg acggcgctgg tgctggccaa actgctgatc ctcgccgcgt
     2821 cgctgtcgga gtcgaacaat cttgtctgga tcgggccgct gaccgtgtgg tcggtggtgt
     2881 ccttcttcct cgagctcatt gtccaattgc ccgaaacaga cgcgttcctg accgtctctt
     2941 ggctggtttg tgcggcattc gcactcgtcg tcataggcgc acgcccgcga ttgcgcggag
     3001 accggcagtt cggcgtgctt attctgatcc ccgtgctgta ctgcgcgcta tttattatcg
     3061 ccggctggct gcggccaatg ctgctggcac gggtggcgac atggctggtg atcccgctgt
     3121 gtttgattct cgcgcgggca gcattgagtc agtccggccg atggcgatcc tatgcggcct
     3181 gtgccgttcc attgctgacc ttcctgttca gcaccgggta ttactaccgt ttccacgaga
     3241 aggaggattg gcggggcgca attcggctta tcgccacgca accgaactgt gcggggccat
     3301 tactggtcag cgaattcaat gcactgggac tgtactatta caagataccg gcacatcggc
     3361 aggcctatgt gtttttgccc gacccgcggc gacggaactc cgtcgaattc agtctcagcg
     3421 aacgccttat gcatctaccg gagcttgatc ccggcgcgat catcgaattc atcgaaaccc
     3481 atcccggtac ggccgtcatc atgcgctggg aatatcgcga agtgatcccg cttgacctgc
     3541 aaaccttgct tgcccacgcg cccaccaaag ctcgtttgga tgggggcctg acggtggctt
     3601 gcttttgatg tcctgatgtg acggcacctg cccgatgcac catacagagg tgggtttttt
     3661 aggatcaggc ctgccggggc tgaccgttgg aggcgctcaa tcccccgtca acaggcaaat
     3721 tgacgccggt aacgaagcgg gcgtcgtcgc tggcaagaaa cgcgatcacg ccggcgatgt
     3781 cgtcgggctg cgccggccgg ccgagcggga tgcgttcctt gaagcgctcc atcagcgcgc
     3841 tgtcctgctc catgtccttc gtcatatccg ttatcgtaaa gctcggattt accgcattga
     3901 cccgaacgcc gcgttcgccg agttccaacg ccagggctct ggtcagattt gtcactgccc
     3961 cctttgctgc gttgtagaag ctcatgttcc agtcgcccct aagtccggaa accgaggacg
     4021 tgttgacgat gcagccacgc gtctccagca gatgcggcag tgcggcccgg cagcagaaca
     4081 acacgccgtc cacgtcggtc gccatgacac gacgccaatc cgcgatgggg gcttccgcaa
     4141 acggaccggc gggcgcaacg ccggcgttgt ttacgagcac gtcgatgcgg ccgaagcacg
     4201 acacggtcgc agcgaccatc gcctc
//
LOCUS       DASWQN010001092         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_370703, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001092 DASWQN010000000
VERSION     DASWQN010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370703"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1513
                     /locus_tag="VGC82_18685"
     CDS             293..1513
                     /locus_tag="VGC82_18685"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEY0425352.1"
                     /translation="MRNPSVTATEMASHAAERTATRAAGRDVVVIQDTSELALGGRRA
                     KANGYGPVGKGGALRGVLLHAVLAVDAGTGGVIGLVDAKVWNRKGGKVAARRSRATPH
                     KESQRWLDGTIRAGEVLAAASHITGVSDRESDIYEHFARRPDNVDLIVRACQNRKIET
                     DDGEQINLLFSHIDGLPERGRFEVKIPAAPGRKARTAELAVRFSTVVLRKPLHGAAAD
                     LPATVTLTMVDVRETSTPEDGKAIHWRLFTTHAVTNLGEARRIVDLYRMRWAIEEFFH
                     TIKTAGFDIEAADIGEPAVMIKFVAAVTVAAVTVMQLVKARDGKTKQRLADAFEPADR
                     PILEALSAQLAGKTERQKNPHRKGSLAFASWVIARLGGWTGYYGKPGPRVMREGLDDF
                     QNIKRGTTLRLKNV"
BASE COUNT          308 a          481 c          522 g          279 t
ORIGIN      
        1 ccttgcccct cacccgaagc cttcgctgcg ctacggcttc gacctctccc cccggaagag
       61 cggctgtcgt attcacacat cttgccctgc ggctgtggaa ctgattcctt gagattgctt
      121 gcgattcgcg ggagttagcc atggctggaa ttgaatacgg actggggtat tttgacgacc
      181 tccggcggca aaaagggggg ccgttttgca tgcagcgctg gtggcgcgtc caagctcttg
      241 tatccgccgg ctggcaagga cacgggcccg ggagattcag ttcacgcggt tcttgcgcaa
      301 tccgtcggtg acagccaccg agatggcgtc gcatgctgcg gagcgcaccg cgacgcgcgc
      361 tgcgggacgt gatgtggtgg tgatccagga taccagtgaa ctggcattgg gagggcggcg
      421 agcgaaggcg aacggctatg ggccggtcgg caagggtgga gcgctgcgcg gtgttctgct
      481 gcatgctgtg ctggcggtgg atgccggcac aggtggggtg atcggccttg tcgacgccaa
      541 ggtgtggaat cgcaagggag gtaaggtcgc cgctcgacgc tcgcgcgcaa ccccgcacaa
      601 ggaatcgcaa cgctggctcg atggcacgat ccgcgccggc gaagtgttgg ctgcagcatc
      661 gcacatcacc ggtgtgtcgg accgcgagag cgacatctac gagcacttcg cgcggcgccc
      721 cgacaacgtc gatttgatcg tgcgagcctg ccaaaaccgc aagatcgaga cggatgatgg
      781 agagcagata aatctgctgt tcagtcacat tgacggcttg cccgaacggg gccggttcga
      841 ggtgaaaatc cccgcggcgc cgggccgcaa ggcgcgtacg gcagaactcg ctgtgcggtt
      901 ctctaccgtg gtgttgcgca agccgctgca cggtgcggcg gccgatctgc cggccacggt
      961 caccctgacc atggtcgatg tccgcgaaac ctcgacgccc gaagatggca aggccatcca
     1021 ctggcggttg ttcacgacac atgccgtcac caaccttggc gaggcgcgca ggattgtcga
     1081 tctctatcgg atgcgctggg cgattgaaga gttcttccat acgatcaaga ccgcgggctt
     1141 cgacatcgaa gccgccgaca ttggcgaacc ggccgtcatg atcaagttcg tcgccgcagt
     1201 cacggtggcc gcagtcacgg tgatgcaact cgtcaaagcc cgcgacggca agaccaagca
     1261 gcgcctcgcc gacgcgttcg agcccgccga tcggccgatc ctcgaagccc tgtcggcgca
     1321 gcttgcaggc aagaccgaac ggcaaaagaa cccgcaccgc aaaggatcgt tagcttttgc
     1381 cagctgggtc attgctcgcc tcggcggctg gaccggatac tacggaaaac caggcccacg
     1441 cgtaatgcgc gaaggtctcg acgacttcca gaacatcaag cgcggcacca ccctgaggct
     1501 gaaaaatgtg tgaatctaac aggctctgcg cggggagagg ttaaggcgcg cgccgccgcg
     1561 cattcactgc gatcgacagc agcgtgcgct
//
LOCUS       DASWQN010001093         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1426362, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001093 DASWQN010000000
VERSION     DASWQN010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426362"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VGC82_18690"
     CDS             complement(<1..433)
                     /locus_tag="VGC82_18690"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0425353.1"
                     /translation="MLGLFKRTRLSDRQEAAVRKTLQNLKAQRRSRFRILACIDGSEG
                     AFNTVRFAARFATTSDCDLIVLYVRPIDQGLHSGGLQVRLARQNMMDAGFELPGVSHL
                     KRALDILKEEGIDAADWTKETENQNAWGDPAGDTKAEYQSPE"
     gene            complement(446..1996)
                     /locus_tag="VGC82_18695"
     CDS             complement(446..1996)
                     /locus_tag="VGC82_18695"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEY0425354.1"
                     /translation="MSIAETTFSLARDSLQAREPSAGLDRFRRRGYQRYRLVRSYIDL
                     SLLWKRRWFIITMVLGFALMSAPTPTGLTHQAQIVLSMSIMATMLFISEAVPLPTVPL
                     LIIVGEVVLLKVDATKVAQSLMTDSVLFIMGSLMLAVAIVKQRLDRRIAWWIVQMTGT
                     NIYWISFGITAVCGILAAFIGEHTVAAMMLPVGVTLITLTSNDPKKVHNLAAVILFSI
                     SYGCSIAGVATPSGGARNAIIISYWKDFFYDPADPATRRYLMDYVSWIIYAFPMFILR
                     LPFVAALLVFTFRPEVTDMSRAITRLRTQVSMQGPLRGSELLTVAIFLATIAGWVMLS
                     STLGLGVIAISGAAAFLVFGLVRWEDINAGVNWGVVLLYAAAISLGVEMEATGAAEWV
                     ASSVFSSMKSMGAASDLGFNTAISLLTIGVSNTMTAGAAVAVLAPIVLKTAVAAGEDP
                     LSAGFVTAISSAFGYLTPAAQPAFTIIYAAGYLKGADFFKIGLRMMLISFVVLLLIST
                     LYWPLLRP"
     gene            2141..>2365
                     /locus_tag="VGC82_18700"
     CDS             2141..>2365
                     /locus_tag="VGC82_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425355.1"
                     /translation="MRDLYTILATAEAEFDRSRERLFDLVRIPSISTDPAHAGDCERA
                     AKAVVAELQALGFDASLRPTSGRPMVVAHYT"
BASE COUNT          510 a          709 c          735 g          411 t
ORIGIN      
        1 cttctgggct ttggtattcc gcctttgtat cgcccgcggg gtccccccag gcgttttggt
       61 tttcggtctc cttggtccag tccgccgcgt cgatgccttc ttccttcaag atgtcgagcg
      121 ctcgcttcaa atggctgacg cccggcagtt cgaagcccgc atccatcatg ttctggcgcg
      181 ccagcctaac ttgcaggccg cccgaatgga ggccctgatc gatggggcgc acataaagca
      241 cgatgaggtc acaatcgctg gtggtcgcaa accgcgccgc gaagcgcact gtattgaagg
      301 cgccctcgga tccgtcgatg caggcgagga tgcggaaacg gctgcgccgc tgggctttga
      361 gattttgcag cgtcttgcgc accgccgctt cctggcggtc ggagagtcgg gtgcgtttga
      421 ataatccgag catcagctcc ccagattaag gtctcagcag cggccagtac agagtcgaga
      481 tgagcagcag cacgacaaaa gagatcagca tcatgcgaag cccgattttg aaaaaatccg
      541 cccccttcag atagcccgcg gcataaataa tggtgaaggc gggctgtgct gcgggtgtga
      601 gatagccgaa ggccgacgaa atggcggtca cgaacccggc gctgagaggg tcttcgccag
      661 ccgccactgc ggtcttcaag acgatgggcg ccagcacggc gacagcggca cccgccgtca
      721 tggtgttgga gacgccgatc gtcagaagcg aaatcgcggt gttgaacccc aaatcgctcg
      781 ccgcccccat ggacttcatg ctgctgaaca cgctgctggc cacccactcg gccgctcccg
      841 tcgcttccat ctcgacaccg agcgaaatgg cggccgcata aagcaacacc accccccagt
      901 tgacgccggc attgatatcc tcccagcgga caaggccaaa caccaggaac gccgcggcac
      961 ccgagatcgc aataacgccg agtcccagcg tcgaagacag catgacccag ccggcgatgg
     1021 ttgcgagaaa aatagcgacg gtcagcaatt ccgagccccg cagtggccct tgcatcgaaa
     1081 cttgggtgcg cagtctggta atggcccgcg acatgtcggt cacttccggg cggaaagtga
     1141 agaccagcag cgccgccacg aacggcaggc gcaggatgaa catcggaaag gcgtagataa
     1201 tccagctcac gtaatccatg agataacgac gtgtcgcggg atccgccgga tcgtaaaaga
     1261 aatccttcca gtagctgatg atgatggcat tgcgcgcgcc gccggaaggc gtcgccacac
     1321 cggcaatcga gcagccatag gatatcgaga acagaatgac ggcggcaaga ttatgcacct
     1381 ttttcggatc gttggaggtg agcgtgatga gggtgacgcc aaccggcagc atcatggcag
     1441 caaccgtgtg ctcgccgatg aaggcagcga gaatgccgca cacagcggtg atgccgaaac
     1501 tgatccaata gatgttggtg cccgtcatct gcacgatcca ccaggcgatg cgccggtcga
     1561 gccgctgctt gacgatggca acggcgagca tgagagagcc catgatgaag agcaccgaat
     1621 cggtcatcag gctctgcgcc actttggtcg catcgacctt gaggagcacg acttcgccca
     1681 cgatgatgag caggggtacc gtgggcagcg gcacggcttc ggagatgaac agcatcgttg
     1741 ccatgatcga catggacagc acgatctgcg cttgatgcgt aagtcccgtg ggcgttggcg
     1801 ccgacatgag cgcgaagccc agcaccatgg tgatgatgaa ccagcgccgc ttccacagca
     1861 gcgataaatc gatgtaggag cggacgagcc gatagcgctg gtagccgcgc ctgcgaaacc
     1921 ggtcgagccc cgccgagggc tcgcgggcct gcaagctgtc gcgggccagt gaaaaagtgg
     1981 tttcggcaat gctcatccca aacacccttg ccgcgcatcg gggcgccgtg agacagtgca
     2041 tgcgattccg cggggcttat caacatctac gcccgtgggt tgagcggcga caagggtcaa
     2101 atagcgccgt ccctaacgcg gggagcagtg aaggagccag atgcgggacc tctatactat
     2161 tcttgccacg gccgaggccg aattcgaccg cagccgggag cgcctgttcg atctcgtccg
     2221 tatcccctcg atttcgaccg atccagccca tgcgggggac tgtgaaaggg cggccaaggc
     2281 agtggtggcg gagctacagg ctttgggttt cgatgcgagc ctgaggccga cctcgggtcg
     2341 ccccatggtc gttgcccact acacg
//
LOCUS       DASWQN010001094         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1556757, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001094 DASWQN010000000
VERSION     DASWQN010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556757"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1517
                     /locus_tag="VGC82_18705"
     CDS             <1..1517
                     /locus_tag="VGC82_18705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0425356.1"
                     /translation="TPVGFLPEDREHVDRTFRHALRTETEWHVECRLRRPDDGTTRWI
                     EVRGRPHRDGGRPTYFLGIVGDITERKAAEGALRRSKELLEDRIAERTRKLTEANAKL
                     RAEAAERERVEDALRQSHKMEAVGQLTGGIAHDFNNMLQAIGGSLELMSRRVEQGRLE
                     EAGRLVATAQTTVERAAFLTHRLLAFARRQSLQPRPVEVDALVCGLIELIRRMAGPQI
                     EIDLRPRDGIWTVMCDPNQLENVLLNLAINSRDAMPNGGTLTIATSDLSLSAPDVAGD
                     EDAQPGDYVEITVSDTGIGMDEMTRRRAFEPFFTTKPLGQGTGLGLSQVYGFVRQSGG
                     IVRLESTLGQGTTVRLYLPRHARVVEADAVPAAKTKPDAEAASAVVLLVEDEALVRST
                     VAATLRDLGYQVLEAGNGPMALQALEGGGRNPVDLLVTDVGLPGGLNGRQVADAARAI
                     KPDLPVLFITGYAGAILEGQLAPGMVMIGKPFTLEAIAAKVKSMIEGSSDGFSC"
BASE COUNT          296 a          483 c          566 g          265 t
ORIGIN      
        1 cgactccggt agggttcctg ccggaggatc gcgagcatgt ggaccgtacg ttccgccacg
       61 ctttgagaac ggaaacggag tggcacgtcg agtgccgcct ccgtcgcccg gacgacggta
      121 cgacgaggtg gatcgaggtg cgcgggcggc ctcatcggga cggcggccga cccacctact
      181 ttctgggcat cgttggcgac attacagagc gtaaggcagc cgagggggcg ctgcgacgat
      241 ccaaggaatt gctggaagac aggattgccg aacgcacccg gaagctgacc gaggcgaacg
      301 caaagctaag ggccgaagcc gccgaacgcg agcgggtcga ggatgcgttg cgacaatcgc
      361 acaagatgga ggcggtcggc caactcacgg gcggcatcgc gcacgacttc aacaacatgc
      421 tccaggccat cggcggcagc ctggaactga tgagccgccg cgtggaacaa gggcggctgg
      481 aggaagcggg ccgtttagtg gcgaccgcgc agacgacagt ggaacgggcg gccttcctca
      541 cgcatcggct gctggctttc gcccgccgcc aatcgctgca gccacgcccg gtcgaggtgg
      601 atgcactggt gtgcggcttg atcgaactga tccggcgcat ggcaggcccg cagatcgaga
      661 tcgacctgcg tcctcgcgac ggcatctgga cggtgatgtg cgaccccaac cagcttgaga
      721 acgtgttgct caatctcgcg atcaattccc gcgacgcgat gccgaatggc ggcacactca
      781 ccatcgccac gagcgacttg tcgctgtcgg cgccggacgt ggctggagat gaggacgcgc
      841 agcctggcga ctacgtcgag atcaccgttt cggacactgg catcggcatg gacgagatga
      901 cccggcggcg tgccttcgag ccgttcttca cgaccaagcc gctggggcag ggcaccgggc
      961 tgggcttgtc ccaggtttat gggttcgtgc ggcagtccgg cgggatcgtg cggctggaga
     1021 gcaccctggg tcagggtacg acggtgcggc tttatttgcc gcggcatgcg cgtgtcgttg
     1081 aagctgacgc tgtccccgcc gcgaagacca agccggacgc cgaagccgcg agtgcggtcg
     1141 tgctgctggt cgaggacgag gctcttgtgc gtagcacggt cgcggcgacg ctgcgcgacc
     1201 taggctatca ggtgctggag gccggcaatg gcccgatggc actgcaggcg ctcgaaggcg
     1261 gcggccgcaa tccggtcgat ctgctggtga ccgacgtcgg cctgccaggt ggcctgaacg
     1321 gacggcaggt ggccgatgcc gcacgggcga tcaagccgga tctaccggtt ctgttcatca
     1381 cgggctacgc cggcgccatt ctggaaggcc agctcgcacc ggggatggtg atgatcggca
     1441 agccgttcac gctggaggca atcgcagcca aagtcaaaag catgatcgaa ggttcgtccg
     1501 acggattttc atgctgagcg ggctcgctgc acccggtctc cgaaatagag cgttttcacg
     1561 ctgactggaa aggtgccggc cagttcgcca gcagttctca atgcgattga
//
LOCUS       DASWQN010001095         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1953916, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001095 DASWQN010000000
VERSION     DASWQN010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953916"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="VGC82_18710"
     CDS             <1..1660
                     /locus_tag="VGC82_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEY0425357.1"
                     /translation="ACVELLKAMGMRPDHVILCDTKGVVYQGRTEGMNQWKSAHAANT
                     KARTLSEAMEGTDVFFGLSVKGALTQDMVASMADKPIIFAMANPDPEITPEEARAVSP
                     HAIIATGRSDYPNQVNNVLGFPYIFRGALDVRASTINDAMKIAAAEALAKLAREDVPD
                     EVHTASVGRRLAFGPEYIIPNAFDPRLIARIPPAVAKAAMDSNVARKPLDDLRRYARG
                     LSGRLDPTADALDATMEMVRENPRRVVFAEGEEQKVVRAAVAFRNAGYGQPILIGRED
                     RVRATLDGLGLGGIEGIEIHNARLSGANSKYVDFLYGRLQRQGHLQRDIQRMVNQDRN
                     VFAACMIATGDADAMVTGLTRSASVCLNDIRHAIGPAKGGIPFGLTLLVGMRGKTIFL
                     ADSLVNFRPDASQLADIAIGAAAAARHLGHEPRVALLSHATFGDPLDARAAPMRDAVA
                     ILDQRKVDFEYDGEMSPDVALEPSIRALYPFCRLTGDANVLVVPGLHSAVIAGRLAHH
                     LGGGTTIGPLLIGMEKPVQIVPMDASSGQIVDLACLAAYQAVAQ"
BASE COUNT          341 a          623 c          575 g          327 t
ORIGIN      
        1 cgcctgtgtc gaactgctca aggcgatggg catgcggccg gaccatgtca tcctgtgcga
       61 caccaagggg gtcgtttacc agggccgcac cgagggcatg aaccagtgga aatcggccca
      121 tgccgccaac accaaggctc gtacgctcag cgaggccatg gagggcaccg acgtcttctt
      181 cggtctgtcg gtgaaaggcg cgttgacgca agacatggtc gcctcgatgg ccgacaagcc
      241 gatcatcttc gccatggcaa accccgatcc ggaaatcacc ccagaggaag caagggcggt
      301 cagcccgcat gccatcatcg ccaccgggcg cagcgactat cccaaccagg tgaacaacgt
      361 cctcggtttc ccctatattt tccgcggtgc cctggacgtt cgtgccagca ccatcaacga
      421 cgccatgaag atcgccgcgg ccgaggccct ggcgaagctg gcgcgcgagg atgtgcccga
      481 tgaggtgcac accgcgtcag tcggacgccg tctcgctttc gggccggaat acatcatccc
      541 caatgcgttc gacccgcgcc tgatcgcccg cattccgccc gctgtcgcaa aggcggcgat
      601 ggactccaat gtggcccgca agccgctcga cgacctgcgc cgctatgccc gcggtctgtc
      661 ggggcgactc gatccgaccg ccgacgcact ggatgccacg atggagatgg tgcgggaaaa
      721 cccccgccgc gtggtcttcg ccgagggcga ggagcagaaa gtcgtccgcg ccgcggtcgc
      781 attccgcaac gccggctacg ggcagccgat cctgatcggg cgcgaagacc gggtgcgggc
      841 cacgctcgac ggcctggggc tcggcggcat cgaaggcatc gagatccaca acgcccggct
      901 ttccggcgcc aacagcaaat acgtggattt tctgtacggc cgcctgcagc gccaaggtca
      961 tctgcagcgc gatatccagc gcatggtcaa ccaggaccgc aacgtgttcg ccgcctgcat
     1021 gatcgcgacc ggcgacgccg acgcgatggt aaccggtctt acccggtcgg catccgtctg
     1081 cctcaacgac atacgccatg cgatcgggcc ggcgaagggc ggcatcccgt tcggcctgac
     1141 cttgctggtc ggcatgcgcg gcaaaaccat cttcctggcc gactcgctgg tgaatttccg
     1201 accggacgcc agccaactcg ccgatatcgc aatcggtgcc gccgccgccg ctcgccattt
     1261 gggacacgaa ccgcgtgttg ccttgctgtc gcacgcgacg ttcggcgatc cgttggatgc
     1321 acgcgcggca ccgatgcgcg atgcggtcgc gattctcgat cagcgcaagg tggacttcga
     1381 atatgatggt gaaatgagcc cggatgtggc gctggagcca tccatccgcg cgttataccc
     1441 gttctgccgg ttgaccggcg atgccaacgt gctggtcgta ccgggcttgc actcggccgt
     1501 catcgccggt cgcctggctc atcatctggg cggcggcacc acgatcggcc cgctgttgat
     1561 cggaatggaa aagccggtcc agatcgtgcc gatggacgct tcatcaggcc agatcgtgga
     1621 tctcgcctgc ctggctgctt accaggctgt cgcccagtga gaatctgctc tggtaatcaa
     1681 ccgcttcagc ggccgattac agatagggcg ttctcacgct gactgaaaaa gtgccggcca
     1741 gttcgccgct cgacgcctct atgtcatggc cgtgctcggc gcggtcaccc acgacttcct
     1801 ttgaagccgc gagagaaccc ctgtttcagt cacagtgaaa acactggagc agtttcacgg
     1861 tgaccg
//
LOCUS       DASWQN010001096         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_898695, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001096 DASWQN010000000
VERSION     DASWQN010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898695"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2277)
                     /locus_tag="VGC82_18715"
     CDS             complement(<1..>2277)
                     /locus_tag="VGC82_18715"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0425358.1"
                     /translation="AFVDGRTVHVHDVTTLIDSDFHDSREIQARHGFRTVLGVPMLRD
                     GVSIGTIGVYRKQVQPFSTAEIRLVETFAAQAVIAIENVRLFNETKEALEQQTASAEV
                     LQVISGSMADASPVFEKIMRSCERLFDGAHAVLSLVRDDGQVYHHSSSTNDPERVREL
                     DRDFPRPLEQSYQSYPLRKKRVVHYPDMANGPGVPQAMRDFAKRIGNVSMLIAPMLWE
                     GKGIGTIHVVRHPPVPFTDKDAALLKSFADQAVIAIQNARLFNETKEALERQTATAEV
                     LQVISSSVADTAPVFDKILDSGQHLFATEQLGIFLVGDDNQVHASAWRGSALEAIART
                     FPKPLEHTMTERVIRERRTIHLPDTAALRDAPVAVLDVVDLIGNCSIAWAPMLWEDRG
                     VGAFAVLRQPPRAFTDKELELLKTFADQAVIAIQNARLFKQAQEARAAAETANEAKSS
                     FLATMSHEIRTPMNAVIGMSGLLLDTKLDTEQHDYVATIRESGDALLTIINDILDFSK
                     IEAGRMDIEAQPFDLRECVESALDLVTARAVDKHLDTAYVFEGDVPAAIVGDVTRLRQ
                     VMLNLLSNAVKFTETGEVVLTVTAKPTSADHVELAFSVRDTGIGLSPEVIGRLFQSFS
                     QADSSTTRKYGGTGLGLAISRRLSELMGGSLWAESEGPGRGSMFHFTIQAPTAALPPA
                     RQRDFVGVQPELQGKRVLVVDDNETNRRVLALQTGKWGMQSRATGSPLESLRWLEQGD
                     AFDVAILDMHMPEMDGLA"
BASE COUNT          410 a          725 c          711 g          431 t
ORIGIN      
        1 gcgccaggcc atccatttcg ggcatgtgca tgtccaggat cgcgacgtcg aaagcatccc
       61 cctgttcgag ccagcgcaac gattcgagcg gtgaaccggt ggcgcgcgac tgcatgcccc
      121 atttgccggt ctgcagggct agcactcgcc ggttggtctc gttgtcatcg accactagca
      181 cgcgcttgcc ctgcaactcg ggttgcacgc cgacaaagtc gcgctgccgt gccggcggca
      241 gcgcggcggt cggcgcctga atggtgaaat ggaacatgga gccccgtccg ggcccctcgc
      301 tctctgccca caggctccca cccataagct ccgaaaggcg cctgctgatg gcaagcccga
      361 gaccggtgcc gccgtacttg cgcgtagtgg atgagtcggc ttgcgagaac gattgaaaga
      421 gacggccgat gacttccggc gacaggccga tgcccgtgtc gcggacggaa aaagcaagct
      481 cgacgtggtc cgcggaggtc ggcttggcgg tcacggtcag cactacctcg ccggtttcgg
      541 tgaacttcac cgcgttggaa agcaggttca gcatcacctg gcgcagccgg gtcacatcgc
      601 cgacgattgc cgcggggaca tcgccttcga acacgtacgc cgtgtccaga tgcttgtcga
      661 ccgcacgcgc ggtgaccaga tccagcgccg attccacgca ctcgcgcaga tcgaagggct
      721 gcgcctcgat gtccatgcgg ccggcttcga tcttggagaa gtcgaggatg tcgttgatga
      781 tcgtcagcag cgcatcgccg gattcacgga tggtggcgac gtagtcgtgc tgctcggtgt
      841 cgagcttggt atcgagcagc aatccgctca tgccgatcac ggcattcatc ggcgtgcgga
      901 tttcatggct catggtggcc aggaacgagc tcttggcttc gttggccgtc tcggccgcgg
      961 cgcgcgcctc ctgcgcctgc ttgaacagcc gcgcattctg gatggcaatc accgcctggt
     1021 cggcgaacgt cttgagcagt tcgagctcct tatccgtgaa cgccctgggc ggctggcgca
     1081 gcacggcgaa cgctcccacc cctcgatctt cccacagcat cggcgcccag gcgatcgagc
     1141 agttcccgat aaggtcgaca acgtcaagca ccgcgaccgg cgcgtcacgc aacgcagccg
     1201 tgtctggaag gtggatggtg cggcgctcgc gaataacccg ctccgtcatt gtgtgctcga
     1261 gcggcttggg aaaagtacgt gcgatggctt cgagagcgga cccgcgccag gcgctggcat
     1321 gcacttggtt gtcgtccccc acaagaaaga taccgagctg ctcggtcgcg aagaggtgct
     1381 gcccgctgtc gaggatcttg tcgaataccg gcgcggtgtc tgcaaccgag ctgctgatga
     1441 cctggagaac ttcggcggtg gcggtctgcc gctccagcgc ctccttggtc tcattgaaca
     1501 gccgagcgtt ctggatagcg atcaccgcct ggtcggcgaa ggacttgagc agcgccgcat
     1561 ccttgtcagt gaacggcact ggcggatggc gcaccacgtg gatggtgccg atgcccttac
     1621 cttcccacag catgggtgcg atcagcattg aaacgttgcc gatgcgcttg gcgaagtctc
     1681 gcatcgcctg cggcacgccc ggcccgttcg ccatgtcagg gtaatgcacc acgcgcttct
     1741 tgcgcagggg ataagactgg taagactgct ccagaggccg cggaaagtcc cgatccaatt
     1801 cgcgcaccct ttcagggtca ttggtgctac tggaatggtg gtatacctgc ccgtcgtcgc
     1861 gcaccagcga cagcaccgcg tgcgctccat cgaacagccg ctcgcagctc ctcatgatct
     1921 tttcgaagac cggcgatgca tcggccatcg agccgctgat gacctgcaac acttcggcgc
     1981 ttgcggtctg ctgctccagc gcttccttgg tctcgttgaa caggcgtacg ttctcgatgg
     2041 cgatcacggc ctgggcagcg aaagtctcga ccaggcgaat ttcggccgtc gagaacggtt
     2101 gcacctgctt gcgatacaca ccgatggtcc cgatcgagac cccgtctcgc agcatgggca
     2161 cgcctagcac ggtgcgaaag ccatggcgcg cttggatctc ccgcgaatcg tgaaaatcgg
     2221 aatcaatcag cgtagtgaca tcgtgcacat gcacggtacg gccatcgaca aacgccc
//
LOCUS       DASWQN010001097         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_634448, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001097 DASWQN010000000
VERSION     DASWQN010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634448"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VGC82_18720"
     CDS             complement(<1..489)
                     /locus_tag="VGC82_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0425359.1"
                     /translation="MELPSIVPPAHGRAWPEAAADVAGMNWMWLEEAARFATENEVQW
                     PYDLRLHLESGFFEPHPFNEVLGPIRPRGPVNGLVLRRGLKIASWGETDQVDFTFSAA
                     KSYLSLLAGIAVMDGLIADLDEPVGRMVDDGGFDSPQNRGVTWRHLLQNTSEWEGELF
                     GKS"
     gene            complement(560..1864)
                     /locus_tag="VGC82_18725"
     CDS             complement(560..1864)
                     /locus_tag="VGC82_18725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425360.1"
                     /translation="MSTTTIGVDRDAVYRKISWRLLPFVLACYVINYLDRVSIGYAKL
                     QFLGELHLNEAVFGLITSAFFVGYIAFEVPSNLLLLRVGAPATLTRIMVLWGAVIISM
                     MFAQNEYWFYVQRFLLGSFEAGFFPGVLLYLSFWFPNHRRGRATSLFLVGIPLSGLLG
                     GAISGAVMEGMDGVLGLRGWRWLFLVDGLPAVLLGLAAMFVLTDKPANALFLSDAEKQ
                     VVIEDMEADRQARTTAASTTVAETLRNPKVWLMVVVYFTMAFLNTNQSWFPTLLRTVG
                     ARSVTEAGNILAGIWIFAAVFVLLVCRNSDRIGERKWHMLVTGTVATVAYLCFPLAAG
                     SLWATALLVAVAAASGYAVFMVFWTIPPVFLEARGAAMGIGMISALGQFGGLSGPAVV
                     GWAFQETGSIYIGFSVAAATIFVGTLLAVFAIPHTRLRKPTG"
BASE COUNT          410 a          662 c          594 g          309 t
ORIGIN      
        1 ggacttgccg aacaactcgc cttcccattc ggacgtgttc tgcagcaaat gccgccaggt
       61 cacgccgcgg ttttgcgggg aatcgaaccc gccatcgtca accatgcggc cgaccggttc
      121 atccagatcc gcgatcagcc cgtccatgac ggcaatgccg gccagcaggc tgaggtagct
      181 cttggcagcc gagaaggtga aatcgacctg atccgtttcc ccccaggagg cgatcttgag
      241 gccacgccgc agcaccaacc cgttgaccgg ccctcgaggt ctgatcggcc ccagaacctc
      301 gttgaacggg tgtggttcga agaagccgct ttccaggtgc agacgcaaat cgtatggcca
      361 ctggacttcg ttttccgttg caaaccgggc ggcttcctcc agccacatcc agttcatgcc
      421 ggcaacgtcg gccgcggctt ccggccacgc gcgcccgtgc gcgggcggca cgatcgacgg
      481 caactccatc cagtcgcttc cttccactgc ggggcgttcc gcccgttccc gggactatgg
      541 cgcccggaac aggcgatggc taccccgtcg gcttgcgcag acgggtgtgg gggattgcga
      601 ataccgccag cagggtgccg acgaagatcg tcgccgcggc gacactgaac ccgatgtaga
      661 tgctgccggt ttcctggaat gcccagccga cgaccgctgg tccggacagc ccgccgaact
      721 ggccgagggc actgatcatg ccgatgccca tcgcggcccc cctcgcctcc aggaacaccg
      781 gcggtatggt ccagaacacc atgaacaccg catatccgga tgccgccgcg acggcgacaa
      841 gtagcgcggt cgcccaaagg ctgccggccg ccagcggaaa gcacaggtag gcgaccgtcg
      901 ccacggtccc ggttaccagc atgtgccact tccgctcacc gatccggtcg gagttacggc
      961 acaccagcag cacgaacacg gccgcgaaga tccagatgcc ggccaggatg ttgccggcct
     1021 cggtcaccga cctggcgcca acggttctca gcagcgtggg aaaccagctc tggttcgtgt
     1081 tcaagaatgc catggtgaag taaacgacca ccatcagcca gaccttcgga ttgcgcagcg
     1141 tttccgctac cgttgtcgat gccgccgtgg tgcgggcctg gcgatcggct tccatgtcct
     1201 cgatcaccac ctgcttttcc gcgtcggaaa ggaagagggc attggccggc ttgtccgtga
     1261 gcacgaacat cgcggccagg cccagcagaa ccgccggaag gccatccacg aggaacagcc
     1321 agcgccagcc gcgcagaccg agcaccccgt ccatgccctc catcaccgca cccgaaatcg
     1381 cgccgcccag cagccccgac aatgggatcc caaccaggaa caggctggtc gcccggccgc
     1441 gccggtggtt ggggaaccag aaactcagat acagcagcac gccgggaaag aagccagcct
     1501 cgaacgatcc cagcaagaag cgctgcacgt agaaccagta ttcgttctgc gcgaacatca
     1561 tggagatgat gaccgcgccc cacagcacca tgatccgggt caacgtcgcc ggcgcgccga
     1621 cgcgcagcaa caacaggttg gacggaacct cgaaggcgat gtagccgacg aagaaggccg
     1681 aggtgatcag gccgaacacc gcctcgttca gatgcaattc acccaagaac tgcagcttgg
     1741 cgtaaccgat gctgacacgg tcgaggtagt tgatgacgta gcacgccagc acgaacggca
     1801 gcaggcgcca actgatcttg cggtagaccg cgtcgcggtc cactccaatg gtcgtggtgg
     1861 acaattatgg ctcctcccga atgtaagcag gcccggatgt aagcaggatc gtgcgatcag
     1921 gctttgcgtg ccagataacg gcggtaatcg gcttcgctat cgacatcgga aagcg
//
LOCUS       DASWQN010001098         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_239095, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001098 DASWQN010000000
VERSION     DASWQN010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239095"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..>1689)
                     /locus_tag="VGC82_18730"
     CDS             complement(40..>1689)
                     /locus_tag="VGC82_18730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425361.1"
                     /translation="IGSVCAAGPDLGPPVSPRAWWLALALASVVLVVPLALTPMPPLL
                     DYPNHLARMLVLERGAADPVLSAMYVADWHILPNIAIDMLMPPLLRVLPLHVVGRLFI
                     ALALLLPFVGTVVLHRTLFGRRSLWPFAAVLVIYNQILFLGFLNYLIGLGLALFGAAL
                     WIRMAPLPARRLVVAAILAVALFFCHLIALAMYGLLLFAVEMGAWWDRPASQRRRIAT
                     FANSIMWLAVPFVVPAVLYLRSPLADAPGDAAWSGRMKLHGVFGPFITYNLGLDLLAL
                     FAVGIFLAWCWWQGHLLVARVGAIGFGILMVLYALVPFEARGTGFIDQRLPPLGALLV
                     FAITQPFGLKRPSAPVAGVVLAMIAMARLTDIGAVWIGHNRDLLAFQQVIAGIQPAEN
                     VLAVAVRQTDVPQFSAGQPRSRFLRALNLTSIMHLPALVLIERDAFWPLLFTAATKQP
                     LRVRPAYAGLAVEEGLVPSYRALEVAAAGGALPRDAPYLAHWQRDFDFVLLLHAGKLP
                     LQDQPLLPGRLELLKQADVAALYRVRRSDGPSQATPAIAGP"
BASE COUNT          383 a          560 c          542 g          204 t
ORIGIN      
        1 ctcccggccc aggtcgtcag ccgactgcca tggcctggtt cacggccccg caatagctgg
       61 cgtggcttgg cttggtccgt cggagcggcg gacccggtac agcgccgcga catcggcctg
      121 ctttaggagt tccagccggc ccggaagcag cggttggtcc tgtagcggca gcttgccggc
      181 gtggagcagc agcacgaagt cgaaatctcg ctgccaatgc gccagataag gcgcatcccg
      241 cggcaacgct ccgccggccg cggcgacttc caacgctcga tatgatggca caaggccctc
      301 ctctaccgcg agtccggcat aggcgggacg cactcgcagc ggctgtttcg tggccgcggt
      361 gaacagcaag ggccagaacg catcgcgttc gatcaacacc agcgcaggca gatgcatgat
      421 cgaggtcagg ttgagcgccc gcaagaagcg gcttcgcggc tggccggcgc tgaattgcgg
      481 gacatcggtc tgtcgaaccg ccaccgccag gacattctcg gcgggctgaa tccccgcgat
      541 cacctgctgg aacgccagga ggtcgcggtt gtgcccaatc cagaccgcgc cgatatcggt
      601 cagccgcgcc atcgcgatca tggcgaggac gacaccggca acaggagcag aaggccgctt
      661 gagaccgaag ggctgggtga tcgcgaacac caacagcgcg cccagtggcg gcaggcgctg
      721 gtcgatgaac ccagtgcctc gggcctcgaa cggaaccaat gcgtacagca ccatcaggat
      781 gccaaacccg atcgcaccaa ctcgcgccac cagcaggtgc ccctgccacc agcaccaggc
      841 caggaaaatc ccgaccgcga acagggccag caggtccaga cccagattgt aagtgatgaa
      901 ggggccgaac acgccgtgca gcttcatgcg gccggaccag gcagcgtcac ccggcgcatc
      961 agccagtggc gaacgcagat acagcaccgc tgggaccaca aatggtacgg ccaaccacat
     1021 tatcgaattg gcgaaagtcg cgatccggcg gcgctggctt gcgggacgat cccaccacgc
     1081 gcccatctcg accgcgaaca acagcaggcc atacatcgcc aaagcgatga ggtggcagaa
     1141 gaacagcgcg acggccagaa tcgcggcgac caccaacctc ctggccggaa gcggcgccat
     1201 gcgaatccat aacgcggcgc cgaacaacgc cagccccaac ccgatcaggt agttcaggaa
     1261 gcccaggaac aggatctggt tatagatgac caagaccgcg gcgaacggcc acaggctgcg
     1321 ccgcccaaac aaagtgcggt gcaggactac ggtgccgacg aagggcagca gcagcgcgag
     1381 ggcgatgaac agccgcccga cgacatgaag cggcagcaca cgcaacagcg gcggcatcag
     1441 catatcgatc gcgatgttgg gcagaatgtg ccaatccgcg acatacatcg ccgaaagcac
     1501 aggatcggcg gcgcctcgtt cgagcaccaa catccgggcg aggtggttcg ggtagtcgag
     1561 cagcggcggc ataggagtca gcgccagcgg caccaccaat acgacggacg ccagcgcaag
     1621 cgccagccac caggcacggg gcgaaacggg cggcccaagg tctggaccgg cagcgcaaac
     1681 cgagccgat
//
LOCUS       DASWQN010001099         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1557194, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001099 DASWQN010000000
VERSION     DASWQN010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557194"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VGC82_18735"
     CDS             complement(<1..531)
                     /locus_tag="VGC82_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0425362.1"
                     /translation="MIQYDAIILGAGAAGLMCAMRAGQRGRRVLLLDHAPQAAAKILI
                     SGGGRCNFTNTGTVPERFLSDNPHFCRSALSRYTPADFVALVRKHGIAFHEKTLGQLF
                     CDGSAREIVAMLLAECAAAGVELRLGHRVVDVSRPDGFRVETDNGPFEAASLVLATGG
                     LSIPKMGATGLAYDLAR"
     gene            733..>1640
                     /locus_tag="VGC82_18740"
     CDS             733..>1640
                     /locus_tag="VGC82_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEY0425363.1"
                     /translation="MAEPLIIARSGDTDLALLPALANRHGCITGATGTGKTVTLQVLA
                     EHFSRIGVPVFLADVKGDLTGISQPGVLSEGMKARLQSLRLPEPQWQGCPVTLWDVWG
                     EQGHPIRATVSDMGPLLMARLLNLNETQEGVLALAFKVADDNGLLLLDLKDLRALLQF
                     VGDNAARFRTQYGNISPASIGAIQRGLLQIEAQGGEKFFGEPMLDINDLIQTADGNGM
                     VNILAADRLMNSPRVYAGFLLWLLSELFEKLPEVGDRDKPKLVFFFDEAHLLFTGAPQ
                     ALLEKVEQVVRLIRSKGVGVYFVTQNP"
BASE COUNT          298 a          530 c          518 g          294 t
ORIGIN      
        1 ccgagcgagg tcgtaagcga ggccggtggc gcccatcttg gggatggaca acccgccggt
       61 cgccaacacc aggctggcgg cctcgaatgg cccattatcc gtctccaccc ggaacccgtc
      121 cggtcggctg acatccacga cgcgatgccc caaacgcagt tcgacaccgg cagcggcgca
      181 ttccgccagc agcatggcca cgatctcccg ggccgacccg tcgcagaaca attgccccaa
      241 ggtcttctcg tggaacgcaa tgccgtgctt tcgcaccagc gccacgaaat ccgccggtgt
      301 gtaccggctg agtgcggatc ggcaaaagtg cgggttgtcc gacaggaacc gctcggggac
      361 ggtcccggta ttggtgaaat tgcatcgacc gccgccggag atcaggatct tggctgccgc
      421 ttgcggggca tgatccagca gcagcacccg ccgtccacgc tggccggcac gcatcgcgca
      481 cataaggccc gcggcgccgg cacccaggat aatcgcgtcg tattggatca tgaacccggg
      541 tatagcagcc ggccttcgca ccgtgacagg tgggttccct gccttcgtga ccgccccagg
      601 tagcccggaa ctgccaccgt ccgggtttgg cgatacgttc cagctatatc cgcgggaatg
      661 ccagcacttc tgttgacttc atcgccctgc tggccttcac cagagtcacg cattcggggg
      721 gatgacagac ctatggcgga accgttgatc atcgcacgat cgggcgatac ggacctggcc
      781 ttgctgccgg cgctcgccaa ccggcacggt tgcattaccg gcgccaccgg caccggcaaa
      841 acggtcaccc tgcaggtgct ggcggaacat ttctcgcgca tcggggtgcc ggtgttcctg
      901 gccgacgtaa agggggattt gactggcatc agccagcccg gagtgctctc ggagggcatg
      961 aaggcgcggc tacagtcgct gcgactgccg gagccgcaat ggcagggctg ccctgtgacg
     1021 ttgtgggatg tctggggtga acaaggccac cccatccgcg ccaccgtatc cgacatgggg
     1081 ccgctgctga tggctcggct gctcaacctg aacgagacgc aggaaggcgt gctggccctg
     1141 gctttcaagg tcgccgacga caacggctta ctgctgctgg atctgaagga cctgcgcgcg
     1201 ttgctgcagt tcgtcggcga caatgccgcc cgtttcagga cccagtacgg gaacatttcg
     1261 cctgcctcga tcggcgcgat ccaacgcggt ctgctgcaaa tcgaggcgca aggcggggag
     1321 aaatttttcg gcgagccaat gctcgacatc aacgacctga tccagacggc agatggcaac
     1381 gggatggtca acatcctggc cgccgaccgg ctgatgaaca gcccgcgcgt ctatgccggc
     1441 tttctgctgt ggctgctgtc ggaactgttt gaaaagctgc ccgaggttgg cgatcgcgac
     1501 aagccgaagc tggtgttctt cttcgacgag gcgcacctgc tgttcaccgg cgctccgcaa
     1561 gccttgctgg agaaggtcga gcaggtcgtc cggctgatcc gctccaaagg tgtcggcgtc
     1621 tatttcgtca cccagaaccc
//
LOCUS       DASWQN010001100         3313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2087023, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001100 DASWQN010000000
VERSION     DASWQN010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087023"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3183)
                     /gene="carB"
                     /locus_tag="VGC82_18745"
     CDS             complement(<1..3183)
                     /gene="carB"
                     /locus_tag="VGC82_18745"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY0425364.1"
                     /translation="MPKRTDIKSIMIIGAGPIVIGQACEFDYSGAQACKALRAEGYRV
                     ILVNSNPATIMTDPGLADATYVEPITPEIVEKIIAKEKPDALLPTMGGQTALNTAMQL
                     HANGALERHGVELIGAKADVIDRAEDRLKFRDAMAEIGIESPRSAIAHTMEEARAALI
                     TTGLPSVIRPSFTLGGTGGGIAYNKEEFEQIVAGGLEASPTTEVLIEESVLGWKEYEM
                     EVVRDSADNCIIVCSIENVDPMGIHTGDSVTVAPALTLTDKEYQIMRDASIACLRKIG
                     VDTGGSNVQFGLNPADGRMVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LTNDITMVTPASFEPTIDYVVVKIPRFTFEKFPGTPALLTTSMKSVGEAMAIGRSFAE
                     ALQKGLRSMETGLSGLDPIDPPGDGGPDAFRAALSTPTPDRLLVAGQALRAGLSVEDI
                     HEATKFDPWFLRELERIVMAEREVTAHGLPREAGALRRLKAMGFSDQRLAELTGTTQA
                     KIHHARTALGVTAVYKRIDTCAGEFASNTPYMYSTFEGGYGVPVCEADPSDRTKIIIL
                     GGGPNRIGQGIEFDYCCVHAAYALKEAGFETIMVNCNPETVSTDYDTSDRLYFEPLTE
                     EDVIALVRREQQNGTVLGCIVQYGGQTPLKLSQALSRANIPILGTSADAIDLAEDRER
                     FQQLLHRLGLRQPDNGIARSAEAAEEIAERIGYPVVIRPSYVLGGRAMEIVYDRHGLQ
                     RYMREAVRVSGENPVLIDRYLNDAIEVDVDCICDGETVYVAGVMEHIEEAGIHSGDSA
                     CSLPPYSLSPAMITELKSETEALAKALGVVGLMNVQYAIKDDTIYVLEVNPRASRTVP
                     FVAKATGVPIAKIGARVMAGAKLATDFKLDDDSIAPHVAVKEAVFPFARFPNVDTILG
                     PEMKSTGEVMGLDSSFERAFAKAQLGAGVILPMAGRVFLSIKDADKKGLPSLARRLTE
                     MGFSITATRGTAGRIRDAGFPVTVVNKVLEGRPHCVDAIRSGEIQLVINTASTPQSVA
                     DSFAIRRSALTHGVPHYTTLAGARAAVHAIA"
BASE COUNT          552 a          994 c         1143 g          624 t
ORIGIN      
        1 cgcaatcgcg tgaaccgcgg cccgcgcacc ggccagcgtc gtgtagtgcg gcacgccgtg
       61 ggtcagggcg ctgcgccgta tggcgaaact gtcggcaacc gattgcggcg tcgatgccgt
      121 gtttatcacg agctggattt cacctgagcg gatggcgtcc acgcaatgcg ggcggccttc
      181 cagaaccttg ttgaccacgg tgaccgggaa gccggcatcg cggatgcgcc cagcggtgcc
      241 gcgtgtggcg gtgatggaga accccatttc ggtcaggcgg cgggccagcg acggcaggcc
      301 ctttttgtcg gcgtccttga tcgacaggaa gacccggccg gccatcggca ggatgacgcc
      361 ggcgccaagc tgggccttgg cgaaagcacg ctcgaagctg gagtcgaggc ccatgacctc
      421 gccggtcgat ttcatctccg gccccagaat cgtgtccacg ttggggaagc gggcgaacgg
      481 gaaaacggct tccttgaccg cgacgtgcgg ggcgatggag tcgtcgtcca gcttgaaatc
      541 ggtggcgagt ttcgccccgg ccatgacccg cgcgccaatc ttggcaatcg gcacgccggt
      601 ggccttggcg acgaacggaa cggtgcggga ggctcgggga ttgacctcca gcacatagat
      661 cgtgtcgtcc ttgatggcat actggacgtt catcagcccg acgacgccaa gcgccttggc
      721 cagcgcctcg gtttccgact tcagttcggt gatcatggcg ggggacagcg aatagggcgg
      781 cagggaacag gcggagtcgc cggaatggat gccggcttcc tcgatgtgtt ccatcacccc
      841 cgcgacgtaa acggtttcac cgtcgcagat acaatcgaca tcgacctcga tcgcgtcgtt
      901 caggtagcgg tcgatcagca ccggattctc gccggagacg cggacggctt cccgcatgta
      961 ccgttgcagg ccatggcggt catagacgat ttccatggcg cggccgccca gcacgtagct
     1021 ggggcggatc accaccggat agccgatgcg ttcggcgatc tcctccgccg cttcagccga
     1081 acgggcgatg ccgttgtccg gctggcgcaa gccaaggcgg tgcagcagtt gctggaaacg
     1141 ctcgcgatcc tcggccaggt cgatcgcgtc ggcggaggtg cccaggatgg ggatgttcgc
     1201 ccgggacaag gcctgcgaca gtttcaacgg ggtttggccg ccgtactgga cgatacaacc
     1261 cagcacggtc ccgttctgct gttcgcggcg gaccagggcg atgacgtctt cctcggtcaa
     1321 cggctcgaaa tacagccggt ccgaggtgtc gtagtcggtg ctgacggttt cgggattgca
     1381 gttgaccatg atggtctcga agccggcctc tttcagggcg taggcggcat ggacgcagca
     1441 atagtcgaac tcgatgccct ggccgatgcg gttcgggccg ccgcccagga tgatgatttt
     1501 cgtcctgtcc gacggatcgg cttcgcagac cgggacgcca tagccgcctt caaacgtgga
     1561 gtacatgtag ggcgtgttgg aggcgaactc gccggcacag gtgtcgatgc gcttgtaaac
     1621 cgcggtgacg cccagggcgg tccgggcgtg gtggatcttc gcctgcgtgg tgccggtcag
     1681 ttccgcgagg cgctggtcgg agaagcccat cgccttgagg cgccggagtg cgccggcctc
     1741 ccggggcagg ccatgagcgg tgacctcacg ttcggccatc acgatgcgct cgagttcacg
     1801 caagaaccag gggtcgaact ttgtcgcttc gtggatgtcc tcaaccgaaa gcccggctcg
     1861 cagggcctgg ccggcgacca gcaaccggtc cggcgttggc gtggacaggg cggcgcggaa
     1921 agcgtcggga ccaccgtctc cgggcgggtc gatagggtcg agaccggaca ggccggtttc
     1981 catgctgcgg aggccctttt gcaaggcttc cgcgaaactg cggccgatgg ccatcgcctc
     2041 gccgaccgat ttcatgctcg tggtcagcag cgccggagtg ccggggaatt tttcgaacgt
     2101 gaagcgggga attttcacta cgacatagtc gatggtgggc tcaaagctgg ccggcgtgac
     2161 catggtgatg tcgttggtca gctcgtccag tgtgtagccg acggccagtt tggcggcgac
     2221 cttggcgatg gggaaacccg tcgccttgga ggccagcgcg gacgaccgtg acacgcgggg
     2281 gttcatctcg atgacgacca tgcggccgtc ggccgggttc aggccaaact gcacattcga
     2341 cccgccggta tcgacgccga tcttgcgcag gcacgcgatc gaagcgtcgc gcatgatctg
     2401 gtactctttg tcggtcagcg tgagcgccgg agccacggtc accgaatcgc cggtatggat
     2461 gcccatcggg tccacattct cgatcgagca gacgatgatg cagttgtcgg cgctgtcgcg
     2521 cacgacctcc atctcgtact ctttccagcc gagtacggat tcttcgatca gcacttccgt
     2581 ggtgggggac gcttcaaggc cgcccgcgac gatctgctcg aactcctcct tgttgtaggc
     2641 gatcccgccg ccggtgccgc ccaacgtgaa gctcggacgg atgacggacg gcaggccggt
     2701 ggtgatgagg gccgcgcggg cttcctccat ggtatgggca atggcggaac ggggagattc
     2761 gatgccgatc tcagccatgg cgtcgcggaa cttcaaccgg tcttcggcac ggtcgatcac
     2821 gtcagccttg gccccgatca gttcgacccc atgccgttcc agcgctccgt tggcatgaag
     2881 ctgcatcgcg gtattcagcg cggtctggcc gcccatggtc gggagcaggg cgtcaggctt
     2941 ctccttggcg atgattttct cgacgatttc gggcgtgatc ggctcgacat aggtggcgtc
     3001 ggccaaaccg gggtcggtca tgatggtggc tgggttcgag ttcaccagga tgacgcgata
     3061 gccctcggcg cggagcgcct tgcaagcctg ggcgcccgag tagtcgaact cgcaggcctg
     3121 gccgatgacg atcgggccgg cgccgatgat catgatggat ttgatgtcgg tgcgcttggg
     3181 cattggggcc tacggtttag ttgaagacgg gctgaactgt cctgacgcgc ccgaatgggc
     3241 cgcaaagcag aaattggatg attcggtgtc ggccgctgct gagcgcctgg ttgagctcgg
     3301 gcttcgaccg gac
//
LOCUS       DASWQN010001101         4365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1162080, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001101 DASWQN010000000
VERSION     DASWQN010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162080"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..3412)
                     /locus_tag="VGC82_18750"
     CDS             complement(269..3412)
                     /locus_tag="VGC82_18750"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0425365.1"
                     /translation="MIQWVVGTSLRFRFIIVALGLSLIYFGSQRLKDIPIDVFPEFAP
                     PRVEVQTLCLGLSPAEVEEQVTVPIENAMNGVPGVEILRSSSVPQLSSVLAILKHGAD
                     EFAARQLVTERVAIATRSLPTWAAPPVMMPPVSSTSRVMKIGVSSKNRSMMDLSMLAY
                     WNIRLRLLAVHGVANVAIWGEQLKMLQVQVDPEHLAELDLTLDHVMETTADALDVGLL
                     RYSGGAHIGTGGFIDTFAQRLPIHHVLSSTTPETLAQIPVAQRDGKQLVLGDVAQLVW
                     GPQGMVGDAVINDGPGLMLIIEKYPWGNTLEVTRGVEAALDEMKPGLPDVEIDTKIFR
                     PAEYIAASVNNLSHALLLGCVLVVIVVFAFLYDVRTALICIVAIPLSLLAAVLVLHWS
                     GSTLNTMVLAGFVIALGVVVDDAILDVENIMRRLRERRAAGDTASTAGIILDASIEVR
                     HPIFYATLIVVIAVVPVFFMQALSGAFFKPLIFAYVMAILASLIVAVTITPVLCLFLL
                     QNASLSGRVSPLSEWLGRIYVWALARATKTPAAAYITVAVVTLAGAATWPFLGYSLLP
                     PFKERDFLIHWIAAPSTSYPEMLRTTKRVSRELRAIPGVRNFGAHIGQAFLADEIVGI
                     ANGENWISIDPNVNYEETVSAIKKVVDGYPGLYRGVETYLDERIQEVLTGDTEPIVVR
                     IFGSDLNVLREQAELVRQALTTIKGIDEVRKDINVDVPHIQVTEKTAEALRYGLKPGD
                     LRRASAVLMAGEEVGDIFADGRAYDVQLWTTPEWRHSFNSVAQMLIDTPTGKRVHMNE
                     VADVQILPTPNVIKREGGSRRIDIQAGAEGRDLASVAKDVQKRLETIKFPLGYHAILQ
                     GAYVELQSAQSDLQAFAILAMGGIFLLLQLSFRSWRLAILSFLTLPSALVGGVLAAYA
                     AGGMITLGSLVGFLSVLGIAARNGIMLVDHFQHLERFEGETFGLSMVLRGARERLRPI
                     LMTTGATGLAIVPLVIYGNQPGHEIEYPMAVVILGGLVTSTLLNLFVLPALYLRFGAG
                     TSGAAKIGDDPKLVAAD"
     gene            complement(3436..3918)
                     /locus_tag="VGC82_18755"
     CDS             complement(3436..3918)
                     /locus_tag="VGC82_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425366.1"
                     /translation="MSVVPISFEKGGCMMRHSTAGVALLAIFVSLHLAAQQAVAESPA
                     PPKPESQATIKRLGFGISEITLLPEAASRIDIHTSEIAEDPSGRKFTSYSSIMYDLDG
                     DVWVYKLTAPLSFVREQVVVESITRDYVYLKEGPPAGTQVVTVGVPELYGTEVGVNGE
                     "
     gene            complement(3915..>4365)
                     /locus_tag="VGC82_18760"
     CDS             complement(3915..>4365)
                     /locus_tag="VGC82_18760"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0425367.1"
                     /translation="AGLIIAGTFVAMLGGFLATYVAGMTLSLGSFLGLVTVAGLALRN
                     GVMQIRHFQYLEQQEAEPGESVVPRDAGELLPSVIASAITTGLIVLPFAVLGDVAGLE
                     ILHPAAVVILGSLVTSTIITLFIIPALYPRLRAKVATKMPVLTPEIA"
BASE COUNT          929 a         1347 c         1272 g          817 t
ORIGIN      
        1 cgcgcaacct gaaggcgatc atctacctcg tcgcaggcaa gctcaaagtc agcctaccca
       61 cgtgaaacag cgaggaacca gacatcccca gtcaggaaag aggtgaccct tatacgccaa
      121 ccgctcaggc ctccaatcgg gccgatatca atgcgcttaa tctgagacat ggataaatcc
      181 ggtcttggta gatgacagcc caagttttgg catgcgatgc cgtgcggccg tccactccaa
      241 taccaaagcc ggtactatgc gcatagtgtc agtccgctgc cacgagtttc ggatcatcgc
      301 cgattttggc tgctccgctc gtacccgcac cgaagcgcag atacagagcg ggaagcacga
      361 ataggttgag cagggtggag gttaccagtc cacctagaat aacgaccgcc atcggatact
      421 cgatctcatg gcccggctgg ttgccgtaga tcaccagggg cacgatcgca agacctgttg
      481 ccccggtcgt catcaggatc ggcctcagcc gctcccttgc gccacggagg accatgctca
      541 gcccaaaggt ctccccttcg aagcgctcca gatgctggaa gtgatcgacc aacatgatgc
      601 cgttgcgtgc ggctatgccc agcaccgaaa gaaatccgac aagtgatccc aacgtgatca
      661 tgccgcccgc cgcgtatgcc gccagcacgc cgccgaccaa tgctgaaggc agcgtgagga
      721 agctcaggat cgccagtcgc cagctacgga acgacagttg cagcaacagg aagatcccgc
      781 ccatcgccag gatcgcgaat gcctggaggt ccgactgcgc agactgcagc tcgacatagg
      841 ctccctgcag gatggcatga tacccgagtg ggaacttgat ggtctccagg cgcttctgca
      901 cgtctttcgc aaccgaagcg aggtcgcgtc cttcggcacc ggcctgaatg tcgatccggc
      961 gggacccgcc ctcacgtttg atgacattcg gagtcggaag aatctggacg tcggccacct
     1021 cgttcatgtg gacccgcttg ccggtaggcg tgtcgatcag catctgtgcg accgagttga
     1081 agctgtggcg ccattccggc gtggtccaaa gctggacatc gtaggctcgg ccgtccgcga
     1141 agatgtcgcc gacttcctcg cccgccatca ggacggccga ggcacgccgc aggtcccccg
     1201 gcttcagtcc atagcgcagc gcttcggcag ttttctccgt aacctggatg tggggcacat
     1261 ctacattgat gtccttcctg acttcgtcga tccccttgat ggttgtaagt gcctggcgca
     1321 ccagctccgc ctgctcacgc aacacattta ggtcggaacc gaaaatgcgc acgacgatcg
     1381 gctcggtatc cccggttaga acctcttgga tgcgctcatc caaataggtc tccacgccgc
     1441 gatacaggcc gggatagccg tccaccacct tcttgatagc ggaaaccgtc tcctcgtagt
     1501 tgacgttggg gtcgatgctg atccagttct cgccgttggc gatgccgacg atttcatccg
     1561 ccaggaacgc ctgacctata tgggcaccga aattgcgaac gcctgggatg gcacgcagct
     1621 cgcggcttac ccgcttggtg gtgcggagca tttccggata tgacgtgctt ggggcggcta
     1681 tccagtggat caggaaatcc cgttctttga agggtggcag caacgaatac cccagaaacg
     1741 gccaagtcgc agcgccagca agggttacaa cggcgactgt gatgtacgcc gcggccggcg
     1801 tcttggtcgc ccgggccagt gcccagacat agatgcgtcc gagccattcg gagaggggcg
     1861 ataccctgcc ggaaagcgag gcattctgta gcaggaacag gcacagcacc ggcgtgatcg
     1921 ttaccgcgac tatcagtgac gcgaggattg ccatcacata ggcgaagatc agcggcttga
     1981 agaatgcccc ggacagcgcc tgcatgaaga agaccggcac gaccgcaatt acgacgatca
     2041 gtgtggcgta aaagatggga tgacgcacct cgattgacgc atcgaggatg atgcctgccg
     2101 tagaggccgt atcgcccgcc gcgcggcgct cgcggagccg ccgcatgata ttctcgacgt
     2161 cgagaatggc gtcgtccacc acgaccccaa gcgcgatcac gaagccggcc agcaccatcg
     2221 tgttgagcgt cgagccgctc cagtggagga cgaggaccgc tgctagcagc gacagcggta
     2281 tagccacgat gcagatcagc gccgtacgca catcataaag gaacgcaaac accacgataa
     2341 cgacaagaac gcaccccaga agcaatgcgt gggacagatt attgaccgac gccgcaatgt
     2401 attccgccgg tcgaaagatc ttcgtgtcga tctccacgtc gggaagcccg ggcttcatct
     2461 catcaagcgc cgcctcgacg ccacgggtca cctcgagcgt gttgccccac ggatacttct
     2521 ctataatcag catcaagccg gggccgtcgt ttatgaccgc atcgcccacc ataccctgtg
     2581 gcccccagac gagctgggcc acatccccga ggacaagttg cttcccgtca cgctgggcga
     2641 cgggaatctg agccagcgtt tccggggtcg tgctcgaaag gacatgatgg ataggaagac
     2701 gttgtgcaaa agtgtcgatg aagccgcccg taccgatatg cgcgccgccg gaatagcgca
     2761 gaagcccgac gtccagcgcg tccgcggttg tttccatgac atggtcgagc gtcagatcga
     2821 gttcagcgag gtgctccgga tccacctgca cctgcagcat cttcaactgc tcaccccaga
     2881 tcgccacgtt ggcaacccca tgaaccgcaa gcagccgcaa cctgatgttc caatatgcga
     2941 gcatcgaaag gtccatcatg gacctgttct tcgacgagac gccgatcttc atgacccggc
     3001 tcgtcgacga cacgggcggc atcatgacgg gcggggccgc ccaggtcggc aggctccgcg
     3061 tggcgatagc gacgcgctcc gtgacgagct gccgcgcggc gaattcgtcg gcgccatgct
     3121 tcaggatcgc gaggacggac gagagctgtg ggaccgaact tgaccggaga atctcaacac
     3181 ccggcacgcc gttcattgca ttctcgatcg ggaccgtgac ctgttcctcg acttcagccg
     3241 gagaaagtcc gagacaaagg gtctgcacct ccactcttgg cggcgcaaac tccggaaaga
     3301 cgtcaatcgg aatgtctttc agccgctgcg atccgaaata aatgagacta agaccgagcg
     3361 ctacaatgat aaaccggaac cgcaggcttg ttccaaccac ccactggatc atggcttggc
     3421 gtctcctgct gtcgtttatt cgccgtttac gccaacttcc gtcccgtata gctcgggcac
     3481 tcccaccgtt acgacttgcg ttccagccgg tggaccctcc ttgaggtaga cgtagtcccg
     3541 cgtgatggat tctaccacta cttgctcgcg cacgaagctc aggggtgcgg tgagcttata
     3601 cacccacaca tcgccatcga ggtcgtacat gatcgaagag tacgacgtga actttctgcc
     3661 ggacgggtct tcggcgattt cggaggtgtg aatatcaatc cggctggccg cctccggcag
     3721 gagcgtgatt tcgcttatgc cgaaaccaag gcgtttgatc gttgcctgag actcgggttt
     3781 cggcggggct ggggactctg ccaccgcttg ctgcgccgcg aggtgcaggc tcacgaatat
     3841 tgcaagcagg gcgacaccgg ctgtggagtg tcgcatcatg cagcctccct tttcgaagct
     3901 gattggtact acgctcatgc gatctccggt gtgagcaccg gcatcttggt ggcaactttt
     3961 gccctcaggc gcggatacag ggctgggatg atgaacaacg tgatgatcgt cgacgtgacg
     4021 aggctcccaa ggatcaccac agcggccggg tgcaggatct cgagccctgc cacgtcgcca
     4081 agcaccgcaa acggcagaac gatcagccca gtggtgatgg cacttgcaat gaccgacggg
     4141 agtagctcgc ctgcgtcccg cggcacaacg ctttcgcctg gctctgcttc ctgctgttcg
     4201 aggtattgaa aatgccggat ttgcatgacc ccgtttcgta gagccaggcc tgcgaccgtg
     4261 acgagtccca ggaacgatcc gagcgagagc gtcataccgg cgacgtatgt ggctaggaag
     4321 ccgccgagca tcgccacaaa cgtcccagcg atgatgaggc ctgcg
//
LOCUS       DASWQN010001102         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_107675, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001102 DASWQN010000000
VERSION     DASWQN010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107675"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VGC82_18765"
     CDS             <1..1194
                     /locus_tag="VGC82_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425368.1"
                     /translation="TFLERHWSETHRRLTRWVEAVAYGNGLVRLVPYDQDATGRSTRR
                     LRALARGGTYTEDDGLESFKEPAKAIGNPRKLVLVPDEQWRDLENGPRDQVWRGQLDM
                     MAITLPEMYGRFRSFAMLGARIDQSMAHVVWQKIANPRYHAHPLQRALRQSHSPEQGR
                     RLSIRYMFEQRASREMLTTQTADGSTMWQAMCETVVAYFARLSSAGEEAGQFIWSAPR
                     DRQGSVHGVKDTFWNVPHGKPGAYRPFDPHLRLSGKSHGSNKFRDVHGAALLSIVNFT
                     PMQYQMLHAIGLSDDEIFKAHSYALVYQDALRTSLRDPARTEPVHIIVPDRPAAEDLA
                     SLFAECTLERLPDDLIPQCAQRRKPGPQPSGNAIPNAERSRRHRLKQRAAREHKAEHE
                     AESGE"
     gene            1403..>2239
                     /locus_tag="VGC82_18770"
     CDS             1403..>2239
                     /locus_tag="VGC82_18770"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0425369.1"
                     /translation="MSGQLAEGYTMEDDGKTYVIRLREGLKFHNGEPVRAQDAVQSLK
                     RWAGRETFGQTLAKFINEWGVKDDLTIRITLTRPVPIFLEAIARGSASIPFIIPEHIA
                     KTDPFTQITDPTGCGPYKFVKEEFTAGSFAAYARNPDYKPAKGTPNWTSGAKVAHFER
                     LEWIAMPEQATAAAALRSGEIDWYEQVQPDLIPPLRKSGGIVVGTANPTNFNGILRFN
                     HLHPPFNNPAVRRAVLMAVNQKDYMEALTGGDFVVDPNCKSMFPCGTPYGRQVGGGAM
                     GGD"
BASE COUNT          469 a          679 c          699 g          392 t
ORIGIN      
        1 accttcttgg aacgccactg gtcggagacc caccgcaggc tcacgcgctg ggtggaggcc
       61 gttgcttacg ggaacgggtt ggtgcggctg gtgccctacg atcaggacgc caccggccgg
      121 tctacccggc ggctcagggc actggcccgt ggcggcacgt acacggagga cgacggacta
      181 gaatcgttca aggagccagc caaagccatc ggtaacccgc gcaagctcgt gctggtacca
      241 gacgagcagt ggcgcgacct tgagaacgga ccaagggatc aggtctggcg cggccaactc
      301 gacatgatgg cgatcacact gccggagatg tacggccgct ttcgctcgtt cgcaatgctc
      361 ggtgcgcgga tcgaccaatc gatggcgcac gtagtttggc aaaagattgc gaacccacgt
      421 taccatgccc acccccttca gcgggcactc cggcagagcc acagtccaga gcaaggccgg
      481 cgtttgagca tccggtacat gttcgagcag agggcgtccc gcgaaatgct gacgacccag
      541 acagcggatg gttccacgat gtggcaggcc atgtgtgaaa cggtcgtggc gtatttcgcg
      601 cggctttcca gcgcgggcga ggaagccggc caattcatct ggtcggcacc acgtgaccga
      661 caaggcagcg tgcatggcgt gaaggatacc ttctggaatg tgccgcacgg gaaacccggg
      721 gcctaccgac cgttcgatcc acacctgagg ctgtccggga aaagccatgg ctcgaacaag
      781 tttcgagatg tgcatggcgc ggcgctgctg tccatcgtga actttacgcc gatgcaatac
      841 caaatgctgc atgcgatcgg gctgagcgat gacgaaatct tcaaggcgca ttcgtacgct
      901 ctcgtatacc aggatgccct acgaacgtcc ttacgggatc ccgcccgcac ggagccggtg
      961 catatcatcg tccctgatcg cccggcggct gaggacctgg ccagcctgtt tgcggaatgc
     1021 acgctggagc ggctgcccga cgacttgatc ccgcaatgcg ctcagcggcg gaagccgggc
     1081 ccgcagccga gtggcaatgc gataccaaat gctgagcggt cgcggaggca tcggctcaag
     1141 caacgggcgg cacgcgaaca caaggccgag cacgaggcgg aaagtggcga ataggtttcg
     1201 gatgtttcag gtcgcgagca gcggcggcag tacgcctagc gagatcttcc gcttgctcgc
     1261 gcggggtcag ctgacggcct gagcgtgacg tggccccacc cttcgttgcc cggttgacac
     1321 ggttttcaac acctctctgg tcaccaccaa tcatggctac gcggccaacg acacgctatt
     1381 cggcatcaat gccaaacgcg agatttcggg ccagcttgcc gaaggctaca cgatggagga
     1441 tgacggcaag acctatgtca tcaggctgcg ggaaggtctg aaatttcaca acggcgaacc
     1501 ggtgcgcgcg caggacgcgg tgcaaagcct gaaacgatgg gcaggccgtg aaaccttcgg
     1561 ccagacgttg gcgaagttca tcaatgaatg gggcgtgaag gatgatctca cgatccgcat
     1621 cacattgact cgcccagtgc cgattttcct ggaggcgatc gcccgtggca gcgcgtcgat
     1681 cccattcatt attccagaac atatcgcgaa gaccgatcct ttcacgcaga tcaccgaccc
     1741 caccggctgc ggtccctata agttcgtcaa ggaggaattc actgccggca gcttcgccgc
     1801 ttatgcgcgc aacccagatt acaagccggc gaaaggtaca ccgaattgga cttccggcgc
     1861 gaaggtcgcg catttcgaac gactggaatg gatcgccatg ccggaacagg ccaccgccgc
     1921 cgccgcgctg cggtcgggtg agatcgactg gtacgaacag gtgcaacccg atctgatccc
     1981 gccactgcga aagagcgggg gcatcgttgt cggcaccgcg aatcccacga acttcaacgg
     2041 catcctgcgg ttcaatcatt tgcatccgcc gttcaataat ccggcggtcc gccgcgccgt
     2101 gttaatggcg gtgaaccaga aggactacat ggaagccctg accggcggcg atttcgtagt
     2161 cgacccgaac tgcaaatcca tgtttccctg cggcacgccg tatggccggc aggtcggcgg
     2221 tggcgccatg ggtggtgat
//
LOCUS       DASWQN010001103         9618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_635218, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001103 DASWQN010000000
VERSION     DASWQN010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635218"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VGC82_18775"
     CDS             <1..603
                     /locus_tag="VGC82_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY0425370.1"
                     /translation="MLAVVALVFLAQLYIAGLPLILVGVAVSLIGGGAVMAYTVLPHV
                     RHRFDGFWKGEGDHYQINTALEAFGNGGFAGRGAGEGHVKDILPDAHADFVFAVAGEE
                     FGMLLCVVILAIFVFIVMRGLLRLLREQELFIVLSCSGLVTGFGLQAFVNMASTLGLI
                     PTKGMTLPFISYGGSSVVAVALSMGMLLGLTRRRGRDEPP"
     gene            600..1745
                     /gene="murG"
                     /locus_tag="VGC82_18780"
     CDS             600..1745
                     /gene="murG"
                     /locus_tag="VGC82_18780"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY0425371.1"
                     /translation="MRGPAVRPIVIAAGGTGGHFFPAEALAAELVTRGHRIVLMTDAR
                     SGGLSSAIFSAREQFIIRGAGIAGRGAWRGAKAIGSLAGGVAQARGILVRLGAGCVVG
                     FGGYPCVAPILAAGLLRHRPCVILHEQNAILGRANRFLARRADLLALSFAATERVPAR
                     TSTEVTGNPVRPAIAALAKTSYVAATDRIRLLVLGGSLGARVFSDVVPAALRSLPDQL
                     RARVTVVQQTRPEDLDRVAAAYAECGIQVELAPFFPDVAGRLVAAHLVIARSGASTVA
                     ELAVAGRPAILVPLPGAIDDHQSANARALQQAGGAWVTPQAEFTPAFLARRLAEILAD
                     PAGLTKAAVGARSQARADAASRLADLVEAHVASVEAGIITNHAGERA"
     gene            1742..3175
                     /gene="murC"
                     /locus_tag="VGC82_18785"
     CDS             1742..3175
                     /gene="murC"
                     /locus_tag="VGC82_18785"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY0425372.1"
                     /translation="MRALPLSIGIIHFVGIGGIGMSGIAEVLHTLGYAVQGSDIAESA
                     NVLRLRESGIPVAIGHDAANLGEARVVVISTAVKRDNPEVAAARARLIPVVRRAEMLA
                     ELMRLKWAVAVGGTHGKTTTTSLIACVLEAAHLDPTVINGGIINAYGTNTRMGSGDWM
                     VVEADESDGSFLRLPAIIAVVTNMDPEHLDHWGSNQAMVAAYDQFVANIPFYGFAVLC
                     IDHPAVQQMIPRLSDHRIVTYGFSPQADVRAERVSPDKFGSTFDVVVTDRFRNRSRRM
                     GPFRLPMLGAHNVQNALAAIAVGIEMEIDDATLNSAFLGFKGVKRRFTKTGEAGGITV
                     IDDYGHHPVEIAAVLKAARQAGARDVVAVVQPHRYSRLQSLFSDFCTCMNDAGTVIVA
                     DVYAAGEQPIPGITRDALVEGLRARGHRSVVALPNPGRLAEMVHAIARSGDFVVCLGA
                     GSITQWATALPADLAALQAGGKRAAAI"
     gene            3172..4161
                     /gene="murB"
                     /locus_tag="VGC82_18790"
     CDS             3172..4161
                     /gene="murB"
                     /locus_tag="VGC82_18790"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY0425373.1"
                     /translation="MTAAARILGLVEGLPALRGRATAKAALAPFTWFRAGGAAEILVR
                     PADADDLAKFLHELPHEIPVQVIGACSNLIVRDGGLTGVTVRLARGFSAIAPEAGGIV
                     AGAAALDVTVAEHAAAAGLTGLEFLSGIPGSIGGAVAMNAGAYGGEISDVLDWAEIVT
                     RTGEQRRLLGPQLAFAYRHAALPAGAIVTRVRLRATQGAPALIAARMAEIRSAREASQ
                     PVRARTGGSTFRNPDGMKAWELIDIAGCRGLTRGGAMVSEKHCNFLINTGTATAADIE
                     GLGEEIRRRVLAATGVTLEWEIRRIGVPAGKPEVLPAGTRAEVLPAPAREDVP"
     gene            4158..5078
                     /locus_tag="VGC82_18795"
     CDS             4158..5078
                     /locus_tag="VGC82_18795"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY0425374.1"
                     /translation="MTRVVVLYGGISAEREVSLSTGIQVIAALREAGFEVAPVVVGQD
                     LGAVIAALTPKPDAVFNALHGRFGEDGAIQGVLDWFGIPYTHSGVRASSIAMDKQAAK
                     ALFLSAGLPVALGRLVSIEDLRTSDPLPLPYVVKPVNEGSSVGVTVIRGGDNRRAQIA
                     RSWTFGPTALVEEYIPGRELTVSVLDDRALAVTEIRAIAGTFYDYESKYIEGGSRHII
                     PADVHPDVYTQAMDVAVAAHRIIGCRGATRCDFRYDDTAGEPGRLVLLEINTQPGLTQ
                     TSLLPEQAAYRGISFQQLCTWMVENAACRA"
     gene            5066..6097
                     /locus_tag="VGC82_18800"
     CDS             5066..6097
                     /locus_tag="VGC82_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY0425375.1"
                     /translation="MSRVKRLPRNATRNSPRNSVYDRPSGWRLMLRRQRRLLRPAAWF
                     AAASAAVVTIVIAGHSLGNRGGPSSTVSTMRERLGAVAAAFGLRVTDVVIEGRENTPE
                     PLLRAAIGVSKGDSILGFSLEDTRSRIEMIPWVERATVERRLPGTLVVNLQERRPFAV
                     WQNQGKYVLVDRAGQVVANQDVSQFRHLPLIVGSGAPGAAKDILDALRDRPSLAEKVS
                     ASVRVGERRWNLRMTSGTDVMLPEGHEAVALDRLIQLQQDRAVLDRPLVAIDMRLPDR
                     LVFRPKLEPKDGLPTKDALPTKDGPPTKDGPIVKDGLTKDAPTTKDGRTSPPGSNPAP
                     VPVLAKKPT"
     gene            6110..7411
                     /gene="ftsA"
                     /locus_tag="VGC82_18805"
     CDS             6110..7411
                     /gene="ftsA"
                     /locus_tag="VGC82_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY0425376.1"
                     /translation="MADNPRRVLPPSDTGPPEAEPSRHLRSGLFGVLDVGTTKIACLI
                     GRVEGDGTLRVLGFGWQKGRGLNGGDITDLEAAERAIRTCVGQAEDMADTRLSSVTVN
                     LSCGQPASRLFNVQWPAGGRAVTENDVRGVLNEGRARAAVDGREPIHVLPLSFAVDAT
                     DNVIDPRGLFCSTLNARLHVIDAAATALRTLESCIHRCDLEIEDLVSAPMASGLSTLV
                     PEERELGATIIDMGGGTTSMAVYADRQMMHTAQLQIGGLHVTKDLAIGLSTTMVHAER
                     LKTLFGNVASSPDDEREMLPVPLVGEEDHQLAKVPRSMVVNIIRPRIEETLEFIKDRL
                     DASEMGRAAGNRVVLTGGACQLAGVRELAARMLGRQVRLGRPAALRGLPELASGPAFS
                     TAAGLLSWAAGDGRSFQDINLNGEPQAGLFRRLVNFLKERV"
     gene            7485..9008
                     /gene="ftsZ"
                     /locus_tag="VGC82_18810"
     CDS             7485..9008
                     /gene="ftsZ"
                     /locus_tag="VGC82_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY0425377.1"
                     /translation="MTLNLTIPTMSHTDFTPRITVIGVGGGGTNAVDNMIAANLQGVE
                     FVVANTDAQQLMHSRADRRIQLGPHITQGLGAGAKPEIGKAAAEEAADELYRHLDGAH
                     MVFITAGMGGGTGTGAAPVIARMARERNILTVGVVTKPFGFEGVRRGRAADQGIEELQ
                     QYVDTLIVIPNQNLFRMANERTTWRDAFKMADQVLYMGVRGVTDLMMVHGLVNLDFAD
                     IRTVMAEMGKAMMGTGEAEGENRAIRAAEAAINNPLLEDTSMSGARGLLINITGGEDL
                     TLFEVDQAANRIREEVDEEANVIFGSAVDQTLEGRIRVSVVATGIESQRSADQRPKLV
                     AVGGGAAPAPQPIVNRNAAPAGAAPAFMPNAIQAPSLLRQVSPQDEAASSPAPEGQGQ
                     QPRAVPQARTQPLPLAQLTAKANLFAESQRPAAMPAHAPVQQLPDQGRPSLFNAVTGA
                     FRRRTTPAFGPVAQPQPMHRETAPEEYQPQTSQVSVQQTSADQTGIEIPAFLRRQHS"
     gene            complement(9310..>9618)
                     /locus_tag="VGC82_18815"
     CDS             complement(9310..>9618)
                     /locus_tag="VGC82_18815"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0425378.1"
                     /translation="DGNLAGRVGILHPADWPEPELSWTLAAPFWGRGLATEAAAEVRR
                     WVFERFNWDRMVSYISPDNTRSRRVAEKLGAVQDGQIMLRGFAAEIWVHPAPGRGVLA
                     "
BASE COUNT         1573 a         3102 c         3266 g         1677 t
ORIGIN      
        1 atgctcgccg tggtcgccct ggtgttcctg gcacagctct acatcgccgg actgccattg
       61 atcctggtcg gtgtggcggt cagcctcatc ggcggcggcg ctgtcatggc ctataccgtt
      121 ctgccgcatg tgcgccaccg cttcgacgga ttctggaagg gtgaaggcga ccactaccag
      181 atcaacacgg cgcttgaggc attcggcaat ggcgggttcg ccggccgggg cgcaggcgag
      241 ggccatgtca aagatatcct tccggacgca cacgcggact tcgtcttcgc ggtcgccggc
      301 gaggaattcg gtatgctgct ctgtgtcgtc atcctcgcca tattcgtctt catcgtgatg
      361 cgcggcctgc tgcgcctgct gcgcgagcag gaactgttca tcgtcctgtc ctgcagtggg
      421 ctggtaaccg gcttcggctt gcaggcgttt gtcaacatgg cttctacgct gggcttgatc
      481 ccgaccaagg ggatgaccct gcctttcatt tcctatggcg gctcctcggt ggtcgccgtt
      541 gccctcagca tgggcatgtt gttggggctg acgcgtcggc gcggccggga tgaaccgcca
      601 tgagggggcc tgcagtacgg ccaatcgtca tcgcggccgg cgggaccggc ggacacttct
      661 ttcccgccga ggcgctggca gccgaactcg tgacgcgcgg gcatcgcatc gtgctgatga
      721 ccgatgcgcg gtccggcgga ctgtccagcg cgatattctc ggcccgcgag cagttcatca
      781 tccgcggcgc cggcatcgcc gggcgcggcg cctggcgggg ggcaaaggca atcggttcgc
      841 tggccggcgg cgtcgcacag gctcgcggca tcctggttcg gcttggtgcc ggctgtgtcg
      901 tcggcttcgg cgggtatcca tgtgtggcgc cgatcctggc tgccggcctg ctgcggcatc
      961 ggccttgcgt catacttcac gagcagaacg cgatcctggg gcgggcgaac cgcttcctgg
     1021 cgcggcgcgc cgatcttctc gcgctgtcct ttgcagccac ggagcgtgtt ccggcacgca
     1081 cgtcgaccga ggtaaccggg aacccggtgc ggccggcgat cgccgcgctg gccaagacca
     1141 gctacgtcgc cgccaccgat cgcatcaggc ttctggtgct tggcggatcg ctgggcgctc
     1201 gggtcttcag cgacgtggtg ccggcggcgc tgcggtctct gcccgaccaa ctgcgggcgc
     1261 gggtcaccgt ggtgcaacag acgcggccgg aagatctgga tcgggtggcg gcggcatacg
     1321 ccgaatgcgg gatacaagtc gaattggcgc cgttctttcc cgatgtcgcg ggccggctgg
     1381 tggccgccca cctggtgatc gcccgaagcg gggcttccac ggtcgccgaa ttggcggtcg
     1441 ccgggcggcc ggcgattctg gtgcctttgc ccggggcgat cgacgaccat caatcggcaa
     1501 acgccagagc cttgcagcag gccggcggcg cctgggtcac accccaggct gaattcaccc
     1561 cggccttcct ggccaggcgc ctggcggaaa tattggccga tccggcggga ctgacgaaag
     1621 cggcggtcgg cgcccgtagt caggcgcggg ccgacgcggc atcccggttg gccgatctgg
     1681 tcgaggcgca cgtcgcctcg gtcgaagcag ggataatcac gaaccacgct ggagagcgcg
     1741 catgagggca ttgccgcttt ccatcggcat catccacttc gtcggcatcg gcggcatcgg
     1801 catgtccggt atcgccgagg tgctgcatac ccttggttat gcggtgcaag gcagcgacat
     1861 tgccgagtcc gccaatgtgc tgcggctgcg cgaatcaggc attcccgtcg ccatcggcca
     1921 cgatgccgct aaccttggcg aggcgcgggt cgtcgtcatc tcgaccgcgg tgaagcgcga
     1981 caaccccgag gtcgccgccg cccgagcgcg gctgattccg gtggtccggc gggccgagat
     2041 gctggcggaa ctgatgcggc tgaaatgggc ggtcgcggtg ggcggcacgc atggcaagac
     2101 cacgaccacc agcctgatcg cctgcgtgct ggaagccgcc catctggacc ccacggtcat
     2161 caacggcggc atcatcaacg cctatggcac caacacccgc atgggcagcg gcgactggat
     2221 ggtggtggag gcggacgaaa gcgatggctc gttcctgcgc ctgccggcga tcatcgcggt
     2281 cgtcaccaac atggacccgg agcatctgga tcattggggc agcaaccagg caatggttgc
     2341 cgcctacgac cagttcgtcg ccaacatccc gttctatggc ttcgccgtgc tgtgcatcga
     2401 tcatccggcg gtgcagcaga tgatcccaag gctgtcggat caccggatcg tcacctacgg
     2461 cttctcgccg caggccgatg tgcgggcaga gcgagtgtcg ccggacaaat ttggttccac
     2521 cttcgacgtc gtggtcaccg accgcttccg caaccgctcc cgccggatgg gtccgttccg
     2581 cctgccgatg ctgggcgcgc acaatgtgca gaacgcgctg gcggcgatcg ccgtcgggat
     2641 cgagatggag atcgacgacg cgacgttgaa cagcgccttc ctggggttca aaggcgtcaa
     2701 acggcggttt actaagactg gcgaagccgg cggcatcacg gtcatcgacg attacggcca
     2761 ccatccggtg gaaatcgccg cggtgctgaa ggccgcacgt caggcaggcg ctcgggatgt
     2821 ggtggcggtg gtgcagccgc accgttactc gcggctgcaa tcgctgttca gcgatttctg
     2881 cacctgcatg aacgatgcgg gcacggtgat cgtcgccgat gtctatgccg ccggagagca
     2941 gccgatcccc ggcatcaccc gcgacgcgct ggtggagggc ctgcgggccc ggggccaccg
     3001 cagtgtcgtt gccttgccga atcctggccg gctggccgag atggtgcatg ccatcgcccg
     3061 ttctggcgac ttcgtcgtgt gcctcggcgc cgggtcgatc acccagtggg caacggcgct
     3121 gccggccgac ctggccgcat tacaggccgg cggcaagcgg gcggcggcga tatgacggcc
     3181 gccgcgcgca tcctcggttt ggtcgaagga ctgcccgcat tgcgcgggcg cgctacggct
     3241 aaggcggcgt tggctccgtt tacctggttt cgggccggcg gcgcggctga aatactggtg
     3301 cgcccggcgg atgccgatga cctcgcgaag ttcctgcatg aactgccgca cgaaataccg
     3361 gtgcaggtga tcggtgcgtg ctccaacctg atcgttcgcg acggcggcct gaccggcgtg
     3421 acggtccgtc tcgcacgcgg attttccgcc atcgcgcccg aagccggcgg catcgtggcc
     3481 ggcgccgcgg cgctggacgt gactgttgcc gaacacgccg cggccgctgg cctgaccggt
     3541 ctggagttcc tatccgggat tcctggatcg atcggcggcg cggtggcgat gaatgccggg
     3601 gcttacggcg gcgaaatctc cgatgtcctg gattgggcgg aaatcgttac tcgtaccggc
     3661 gaacagcggc gactgctggg accccagctg gcgttcgcct atcgccatgc cgctttgccg
     3721 gcgggggcga tcgtcacgcg cgtacgtctg cgggcgacgc aaggcgcacc ggcgctgatc
     3781 gccgcccgca tggccgagat caggtcggcg cgcgaagcgt ctcagccggt acgcgcccgc
     3841 accggcggat cgacgttccg caacccggac gggatgaagg cctgggaact gatcgacatt
     3901 gccggctgcc gcggcctgac gcgcggtggg gcgatggtgt ccgaaaagca ctgcaatttt
     3961 ctgataaaca ccggcaccgc gacggcggcc gatatcgagg gtctcggcga ggaaatccgc
     4021 cgccgcgtgc tggccgcgac cggcgtcacc ctggaatggg aaatccgccg catcggcgtt
     4081 ccagccggca aacccgaagt gttgccggca ggcacgcggg cggaagtgtt gccggcgccg
     4141 gcccgggagg atgtcccatg acccgcgtcg tggttctgta cggcggcatt tccgccgaac
     4201 gggaggtcag cctgtccacc ggcatccagg tgatcgcggc gctgcgtgag gctggattcg
     4261 aggtcgcgcc ggttgtcgtc gggcaggatc tcggcgcggt catcgcagca ctgacgccga
     4321 aaccggacgc agtgttcaac gcgctgcacg ggcgctttgg cgaagatggc gccatccagg
     4381 gcgtgctgga ctggttcggt atcccctaca cccactccgg tgtgcgggcc tcttccattg
     4441 ccatggacaa gcaggcggcg aaggcacttt tcctgtccgc cggactgccc gttgcccttg
     4501 gccgcctggt atccatcgag gaccttcgga cgtccgatcc gctcccgctg ccctacgtgg
     4561 tcaagccggt caacgaggga tcgtcggtag gcgtcacggt gatcaggggc ggcgacaacc
     4621 ggcgggcgca gattgcgcga agctggacgt tcgggccgac cgcccttgtg gaagaataca
     4681 tcccgggccg ggaactgacg gtcagcgtgc tggacgaccg tgctctggcg gtgaccgaaa
     4741 tccgcgccat cgccggaacg ttctatgatt acgagtctaa atacatcgag ggtggctccc
     4801 ggcacatcat tccggccgat gtgcatcctg acgtctatac gcaggcaatg gatgttgcgg
     4861 tcgcggcgca ccggatcatc ggctgtcgtg gcgccacccg ctgtgatttc cgttacgacg
     4921 acaccgccgg agagcccggc cggttggtac tgctggagat caatacgcag ccgggtctga
     4981 cgcagacatc gttgctgccg gagcaggccg cctatcgcgg catcagcttc cagcagctct
     5041 gcacctggat ggtggagaat gccgcatgtc gcgcgtgaaa cgtttgccgc gcaacgccac
     5101 gcgcaactcc ccgcgcaact cggtgtacga ccggccctcc ggctggcggc tgatgttgcg
     5161 ccgtcagcgc cggctgctgc gcccggcggc ctggttcgcc gcagcgtctg ccgccgtggt
     5221 gaccatcgtg atcgccggcc attccctcgg caatcgtggc gggcccagtt caaccgtttc
     5281 caccatgcgc gaacggctgg gcgcggttgc cgctgcattc ggcctgcgcg tgacggatgt
     5341 cgtgatcgaa gggcgggaaa acacgccgga gcctttgctg cgcgcggcga tcggggtcag
     5401 caaaggtgat tccatcctcg ggttctcgct ggaggacacc agatcgagaa tcgagatgat
     5461 cccgtgggtg gagcgtgcaa cggtggagcg ccggctgccc ggcacgttgg tcgtcaacct
     5521 gcaggagcgg cggcccttcg ccgtctggca aaatcaagga aagtacgtgc tggtggatcg
     5581 cgccggacag gtcgtcgcca atcaggatgt ctctcagttc cgccacctgc cgctgatcgt
     5641 tggttcgggc gcaccgggtg ccgccaagga tatcctggac gctctgcggg accggccatc
     5701 gctggcagaa aaggtctcgg ccagcgtgcg ggtcggggag cgccggtgga acctgcggat
     5761 gaccagcggc accgacgtca tgctgccgga gggccacgag gccgttgcac tggaccggtt
     5821 gatccagctt cagcaggacc gcgcggtgct cgaccgcccg ctggtcgcca tcgacatgcg
     5881 cttgccggac cggctggtgt tccgcccgaa actcgaaccg aaagatgggc tgccgacaaa
     5941 ggatgcgctg ccgacgaagg atgggccgcc gacgaaggat gggccgatcg tcaaggacgg
     6001 gctgacaaaa gacgcgccaa caacgaaaga cggacgcacc agtccgcccg ggtcgaaccc
     6061 tgcaccggtt cccgttcttg ccaagaaacc tacctaggag cgcgccctga tggctgacaa
     6121 tcctcgtcgc gtgcttccac cctccgatac cggtccgccg gaagcggagc cttcgcggca
     6181 tcttcgctcc ggtctgttcg gcgtgctgga tgtcggaacc accaagatcg cctgcctgat
     6241 cggacgcgtc gagggcgacg gcacgctgcg tgtgctgggt ttcggctggc agaagggccg
     6301 ggggctgaac ggcggcgata tcaccgatct ggaagctgcc gagagggcga ttcgcacctg
     6361 tgtcggccag gcggaggaca tggccgatac cagattgagc tccgtcaccg tcaacctgag
     6421 ttgtgggcag ccggcgtcgc gcctgttcaa cgtgcagtgg ccggccggcg ggcgcgcggt
     6481 gaccgagaac gacgttcgcg gcgttctgaa cgaaggccga gcccgggcgg ctgtggatgg
     6541 ccgcgagccg atccatgtcc tgccgctgag tttcgcggtc gatgccaccg acaacgtcat
     6601 cgacccgcgc ggcctgttct gctccacctt gaacgctcgg ttgcacgtca tcgatgccgc
     6661 ggcgaccgcg ttgcggacgc tggagtcctg catccaccgc tgcgaccttg aaatcgagga
     6721 tctggtttcg gcgcccatgg catccggttt atccaccttg gtgccggagg aacgcgaact
     6781 cggcgccacc atcatcgata tgggcggtgg caccaccagc atggcggtgt acgccgaccg
     6841 gcagatgatg cacaccgcgc agcttcagat cggcgggctg cacgtcacca aggatctggc
     6901 gatcgggttg tccacgacaa tggtccacgc cgaacggttg aagacgctgt tcggcaacgt
     6961 ggcatcaagc ccggacgacg agcgggaaat gctgccggtg ccgctggtcg gcgaagaaga
     7021 ccatcagctc gcgaaagtgc cgcgcagcat ggtggtgaac atcatccgtc cgcgcatcga
     7081 ggaaaccttg gagttcatca aggaccgcct cgacgcgtcg gagatgggcc gagccgccgg
     7141 caatcgcgtg gtgctgaccg ggggtgcttg tcagcttgcc ggcgtgcgtg agctcgccgc
     7201 ccggatgctg ggccgccagg ttcgccttgg ccgtccggct gccttgcgcg gtttgccgga
     7261 gctggcctcc ggcccggcct tttccaccgc tgccggcctg ctctcctggg cagcaggcga
     7321 cggccgaagt tttcaggaca tcaacctgaa tggagaaccc caggccggtc tgttccgccg
     7381 gcttgttaat tttttaaagg aacgtgtctg actccgtagc gaatcgaaag tttcgtcgta
     7441 acaagcaacc gtagccgtcc cgttaacccg ggggagagat ttaaatgacc ctgaacctga
     7501 cgatcccgac catgagccac accgatttca cgccccgaat caccgtcata ggcgtcggcg
     7561 gcggcggaac caacgcggtc gataatatga ttgccgccaa cctccagggt gtggagttcg
     7621 tggtcgcgaa caccgatgcg cagcagctta tgcatagccg cgccgaccgc cgcatccagc
     7681 ttggtccgca catcacgcag ggcctgggcg ctggcgcgaa gccggagatc ggcaaggcgg
     7741 ccgcggaaga ggcggcggac gagctgtatc gtcatttgga tggcgcccac atggtgttca
     7801 tcaccgcagg catgggcggc ggcacgggta ccggcgcggc acctgtcatc gcccgcatgg
     7861 cgcgcgaacg taatatcctg accgtcggcg tcgttaccaa gccgtttggt ttcgagggcg
     7921 tgcggcgcgg cagagccgcc gatcagggca tcgaggagct gcagcaatac gtcgatacat
     7981 tgattgtgat cccgaaccag aacctgttca ggatggccaa cgagcgcacg acctggcgcg
     8041 acgccttcaa gatggccgat caggttcttt acatgggcgt tcgcggcgtg accgacctga
     8101 tgatggtcca cggcctggtg aacctcgatt tcgccgacat ccgcacggtg atggcggaga
     8161 tgggcaaggc catgatgggc accggcgagg ccgagggtga gaaccgcgcc atccgcgccg
     8221 ccgaggctgc gatcaataat ccgttgctgg aagacaccag catgtccggc gcgcgtggcc
     8281 tgctgatcaa catcaccggc ggcgaggacc tgacgctgtt cgaggtcgat caggcggcga
     8341 accgcatccg tgaggaagtg gacgaggaag ccaacgtgat attcggctcc gcggtcgatc
     8401 agacgctgga gggtcgcatt cgcgtatcgg ttgttgcgac cggcatcgaa tcccaaagat
     8461 cggccgacca gcggccgaag ctggtcgccg ttggcggcgg agcggcaccg gcgccgcagc
     8521 cgatcgtcaa ccggaacgcc gcaccggcgg gagcggcccc cgccttcatg ccgaacgcca
     8581 tccaggcgcc gtcgctgctg cgccaggtgt caccgcagga tgaggcggcg tcctcgccgg
     8641 ctcccgaggg tcaggggcag cagcccaggg cggtgccgca ggcgcggacg cagccgctac
     8701 cgctcgcgca gttgaccgcg aaggccaatt tgtttgccga atcgcagcgt ccggcggcga
     8761 tgcccgcgca tgcgccggtg cagcagttgc cggaccaggg aagacccagc ctgttcaacg
     8821 cggtgactgg ggcattccgg cggcggacga cgccggcgtt cgggccggtg gcacagccgc
     8881 aaccgatgca ccgcgaaaca gcgcccgagg agtaccagcc gcaaacgtcc caggtatccg
     8941 ttcaacagac ctcggcggat cagaccggga tcgagatccc ggcgttcttg cggcgccaac
     9001 attcctgagg gattgagcta aacaggtcat ggccgtgctc cacatgtcat ggccccggtc
     9061 cgattgtcat ggccgtggtc cgttaagtca cggccatggg gctcatgtgt catggtccct
     9121 gtgtcatggg ccgggcttgg cacggccacc cacgacattc tttgcaatca caagagaacc
     9181 ctgatctcca gtcgcggcaa aaccgctttt agcggtttgt gctcacctcg gttcgcggac
     9241 accgccctaa gcccattgtt tgagggcgtg attcgccttt tcggtgactt cgcgtgcaaa
     9301 gatcacgccc taagcaagta ctccgcgtcc cggcgccgga tgcacccaga tttccgcggc
     9361 gaagccgcgc aacatgatct ggccgtcctg gaccgcaccc agcttttcgg cgacccgccg
     9421 tgatcgcgtg ttgtcgggcg atatatagct gaccattcgg tcccagttga accgctcgaa
     9481 aacccaccgg cgcacttccg ccgcggcttc ggttgccagg ccccggcccc agaacggcgc
     9541 ggcgagcgtc caggacaatt ccggttcggg ccagtcggcc gggtgcagaa tgccaacccg
     9601 ccccgccaga ttgccgtc
//
LOCUS       DASWQN010001104         1830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1295668, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001104 DASWQN010000000
VERSION     DASWQN010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295668"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1366
                     /locus_tag="VGC82_18820"
     CDS             260..1366
                     /locus_tag="VGC82_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY0425379.1"
                     /translation="MKIDDVTLTIFTWDNIPVTVYHQGSTASSGSNLGLLRIRTDSGL
                     EGNAFLGSASNPAGMDGPQLIRSLKPMLMGQDPLQRERIHAGMRLINRTVSYRTIGAV
                     DTALWDLAGKIAGLPVHALMGTFRTSIPAYASSQVLPDVGAYVDQAQSFKAAGWRAYK
                     IHPPRDPDHDIRVCQAVRKAVGDDYRLMLDSTWSYDYTDALRVGRAIEEMGYHWFEDP
                     LSDEDIYGYVKLRQKLNIPIMATEFPAGGLDTYAVWLTEKATDYLRGDIPNKGGLTTM
                     LKTAHLAEAFGMKYEVHHSGNSLNNLANLHLCMALRNTTMWEVLLPDGAHKYGMVKEL
                     EIDADGMMHAPTAPGLGGEIDFDLIARKTEAVLK"
     gene            complement(1389..>1830)
                     /locus_tag="VGC82_18825"
     CDS             complement(1389..>1830)
                     /locus_tag="VGC82_18825"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY0425380.1"
                     /translation="HRLAATGNLSPHGLTPDDALALVLLLDQVPRNIYRGSAGAYATD
                     PLARGVARYALERQLDRWIEPALRQFFYIPFQHSEDRSDQARSLRLSAAYHRAHPETS
                     WLRHARHHARLIQRFGRFPHRNPILGRESTPEELDYLSNGGFAG"
BASE COUNT          360 a          562 c          583 g          325 t
ORIGIN      
        1 ctgtggtcgt tcgtgaaact cgttgttctc ggtgttgaaa caagaaagag cgaacgcacg
       61 cgcccgccgc atcaggcccg gctacgctta aaccactccc agcagatgcc cggccccaac
      121 accgcatagc gcccgttcca agaagccagc cacgtgatga accgcttcca gtcggccctg
      181 cggccgttat tatgacaacg ggctgttttc cgcatcggcg atttcatggc agggtgactt
      241 caccaagaag ggaagcacca tgaaaatcga tgacgtcacg ctgacgatct tcacctggga
      301 caacatcccg gtcaccgttt atcaccaggg atcgacggct tcatccggca gcaacctcgg
      361 gctgttgcgg atcaggaccg attcgggcct cgaaggcaat gcctttctcg gttccgccag
      421 caatcccgcc ggcatggacg gcccgcaact gatccggtcg ctgaagccga tgctgatggg
      481 gcaggacccc ttgcagcggg agcgcatcca cgccgggatg cgcttgatca atcgcaccgt
      541 cagctaccgc acgattggcg cggtcgatac cgcgttgtgg gacctcgcgg gcaagatcgc
      601 cgggcttccg gtgcacgccc tgatggggac gttccgcacc tcgatcccag cctatgccag
      661 ttcccaggtg ctgccggacg tcggggcgta tgtcgatcag gcgcaatcct tcaaagccgc
      721 cggctggcga gcctacaaga tccacccgcc gcgcgacccc gaccatgaca tccgggtctg
      781 tcaggcggtg cgcaaggccg ttggcgacga ctaccggctg atgctcgatt cgacctggtc
      841 ctatgactat accgacgcgc tgcgggtcgg ccgggcgatc gaggaaatgg gctatcactg
      901 gttcgaggat ccactttcgg acgaggacat ctacggctac gtcaagctgc ggcagaagct
      961 gaacatcccg atcatggcga ccgagtttcc ggccggcggc ctggacacct acgcggtctg
     1021 gctgaccgag aaggccaccg attacctgcg tggcgacatt cccaacaagg gcggactgac
     1081 cacgatgctg aagaccgccc atctggccga agcattcggc atgaaatacg aagtccacca
     1141 ctccggcaat tcgctgaaca acctggccaa cctgcatttg tgcatggcgc tgcggaacac
     1201 cacgatgtgg gaggtcctgc tgccggatgg ggcacataaa tacggtatgg tgaaggagtt
     1261 ggagatcgac gccgacggaa tgatgcatgc cccgacggcc ccgggactgg gtggcgagat
     1321 cgacttcgac ctgatcgccc ggaagaccga ggcggtgctg aagtaggcaa tgtggcttcg
     1381 ccggttggtt agccggcgaa gccaccgtta ctcaagtagt ctagctcttc cggggtggat
     1441 tcacggccaa ggatcgggtt gcggtggggg aagcggccga aacgctggat cagcctggcg
     1501 tggtggcgtg catgccgcag ccaggaggtc tccggatggg cgcggtgata cgccgcggac
     1561 agtcgcagcg aacgggcctg atcgctgcgg tcttccgaat gctggaacgg tatgtagaag
     1621 aattgtcgca gcgccggttc gatccaccgg tcgagttgtc tttccaacgc ataccgtgca
     1681 acgccgcgcg ccagcgggtc ggtggcgtag gcgcccgccg aaccgcggta gatgtttcgc
     1741 ggcacctgat cgagcagcag caccaacgcc aacgcatcat ccggcgtgag gccgtgaggg
     1801 ctgagatttc ctgtggcggc gagacggtgc
//
LOCUS       DASWQN010001105         3065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1557888, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001105 DASWQN010000000
VERSION     DASWQN010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557888"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..694
                     /gene="rnc"
                     /locus_tag="VGC82_18830"
     CDS             23..694
                     /gene="rnc"
                     /locus_tag="VGC82_18830"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY0425381.1"
                     /translation="MSPSFEAILGHEFHRPVLLTEALTHRSAVQGKGRRQASNERLEF
                     IGDRVLGLIMAEWLAERFPREQEGELGRRLAFLVSQPVLAGVAETVGLAAALSVSPGE
                     AKAGVAKRATVLADALEAALGALYMDGGLGAARDFVRRAWNHAMTLQAEPPKDAKTSL
                     QEWAQKRGLELPSYEITGRSGPPHAPEFIVTVSVGANDAVGTAGTRRAAEQLAAEALL
                     QRLSA"
     gene            691..1602
                     /gene="era"
                     /locus_tag="VGC82_18835"
     CDS             691..1602
                     /gene="era"
                     /locus_tag="VGC82_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY0425382.1"
                     /translation="MTGTPPMRCGFVAIVGAPNAGKSTLMNRLTGAKLSIVSPKAQTT
                     RFRVLGILMRAESQVLLVDTPGIFRPKRKLDRAMVAAAWSGAADADLAMLLVDSRSGL
                     REDVREIARRLGASGRRCWLVLNKTDLVTPPSLLPLIASLGALATFEETFMVSAQTGD
                     GIRRLADALAAVVPEGPWLYPEDDLTDLPDRLLAAETVREQIFLQTHEEVPYGATVET
                     ESFKDRKDGSVRIEVTIYVARSGHKAILIGDKGSKIKAIGARARLDLAALLDRPVHLF
                     LNVKERKGWDEEGARLRAIGLEDPGKA"
     gene            complement(1806..2054)
                     /locus_tag="VGC82_18840"
     CDS             complement(1806..2054)
                     /locus_tag="VGC82_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0106034 - protein maturation by [2Fe-2S]
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEY0425383.1"
                     /translation="MSESLPIVAQKGSYKIEVEAGKSYWWCSCGQSSKQPFCDGSHKG
                     SAFAPMKFDAEEAGVVSFCGCKASGKMPRCDGSHKNLL"
     gene            2238..2999
                     /gene="recO"
                     /locus_tag="VGC82_18845"
     CDS             2238..2999
                     /gene="recO"
                     /locus_tag="VGC82_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY0425384.1"
                     /translation="MEWDAPAIILDVRPYGEGDALATVMTEAHGLHRGLARGGSSRGK
                     AGTWQAGNMVQVHWSARLTDQLGAFTGELIHAGAAHAMQQALSLSMLTSICSVAEGAL
                     PEREPHPGIFDGLLRLIPQLPQGEALLTEVIQWEMVVLADLGYGLDFAMCAVTGRTHG
                     LAYVSPKTGRAVTAEGAGDWASRLLPLPGFITGTTPPDLIGWRDGLRLTGHFLARDAF
                     GNQHRPLPQARAMLYDRVAAMVAGAEAVEHGSVGR"
BASE COUNT          525 a          962 c         1012 g          566 t
ORIGIN      
        1 cctgcttcac ctcgtgcgtt gaatgagccc atcctttgaa gcgatccttg gtcacgagtt
       61 ccatcgcccg gtgctgctga ccgaggcttt gacccatcgc tcggcggtcc agggtaaagg
      121 ccgtcggcaa gcatcgaacg aacgcctcga attcatcggc gaccgggtcc tgggcctgat
      181 catggccgag tggctcgccg aacgcttccc gcgcgagcag gagggcgaac tcgggcggcg
      241 cctggccttt ttggtgtcgc agccggtttt ggccggtgtg gcggaaaccg tcggcctggc
      301 ggcggcactc tcggtttcac ctggtgaagc caaagcgggt gttgccaagc gggccacggt
      361 tctggcggac gctttggagg ccgcgcttgg tgcgctttac atggatggcg ggctcggtgc
      421 ggcccgggat ttcgtgcgac gtgcttggaa ccatgcgatg acgctgcagg cggaaccacc
      481 caaggacgcg aaaacgtcct tgcaggaatg ggcgcagaaa cgagggctgg agttgccctc
      541 ctacgagatc accggccggt ccggcccacc gcacgcgccg gagttcatcg tcaccgtgag
      601 cgtaggtgcc aatgacgctg tcggtaccgc cggcactcgt cgggctgcgg aacagcttgc
      661 agccgaagca ctgctgcaaa ggctttcggc atgaccggca cccctcctat gcgctgcggc
      721 ttcgtggcga tcgtcggcgc cccgaatgcc gggaaatcga cactgatgaa ccggctgacc
      781 ggggcgaagc tgtcgatcgt ctcgccgaaa gctcagacaa cgcgcttccg ggtcctcgga
      841 atcctgatgc gggcggagtc acaggtgctg ctggtcgata cgcccggtat ctttcgcccc
      901 aaacgcaagc tggaccgtgc catggttgcc gcggcgtggt ccggtgccgc ggacgctgac
      961 ctggcgatgc tgctggtgga ttcgcgctcc ggcctgcgcg aggatgtgcg cgagatcgct
     1021 cgacggctag gcgcatctgg gcggcggtgc tggctggtac tgaacaagac cgacctggtg
     1081 acgccaccct cgctactgcc gctgatcgcc tcgcttggcg ccttggcgac gttcgaggaa
     1141 accttcatgg tcagcgccca gaccggcgac gggatacggc gtctggccga tgctctggcc
     1201 gccgttgtgc cggaaggacc gtggctgtac cctgaggacg acctgaccga tctgcccgac
     1261 agactgctgg cggcggagac ggtgcgggag caaatcttcc tccagacgca cgaggaagtc
     1321 ccttacggcg ccacggtgga gacagaatcc ttcaaggacc gtaaggacgg ttcggtgcgc
     1381 atcgaggtta cgatctatgt cgcccgttcc ggccacaagg cgatcctgat cggagacaag
     1441 ggcagcaaga tcaaggcgat cggcgcccgc gctcggcttg atctggccgc tttgctggat
     1501 cgtccggtgc atctgttcct gaacgtcaag gaacgcaagg gatgggacga agagggcgcc
     1561 cggcttcgcg ctatcggcct ggaagatccc ggcaaggctt gatcgagtgg cggcaagacg
     1621 tagccttcct tcgcatctgg ccccttcgca ttggtcggct gcgcacctca gcgtcggcca
     1681 tgagtgcgat ggcagatcgc caccgctgac cggatgaacg ttcccacggc accatggtgg
     1741 gcgcaggccc accatccacg ccttcgatag gtcgcgcacc gaagccgtta ctccggagga
     1801 gactcctaca gcaggttttt gtgcgatccg tcgcaccgcg gcatcttgcc ggacgccttg
     1861 cagccacaga acgacaccac acccgcttcc tcggcatcga acttcattgg cgcgaatgcc
     1921 gaacctttat gcgacccatc gcagaacggc tgcttgctgc tttgcccaca ggagcaccac
     1981 caataggatt ttccggcttc gacctcgatc ttgtaggagc ctttctgagc gacgatcggc
     2041 aatgattcgg acatgacatt cccctgtatt attcgcacaa ccgcagcgcc aggaacctag
     2101 cgtgctttcg ctgagcgcaa cgtagcggtc cggccggtgt tcggaaagag ccacctcgtt
     2161 cgcgccatgg tctgggaagg taggcatcga tcacgagcag gtggcgccgg acgcgtgtcc
     2221 ggcatacaac gcggtacatg gaatgggacg cgcctgcgat catcctcgat gttcggccgt
     2281 acggcgaggg cgatgcgctg gcaacggtga tgaccgaggc gcacggtctt catcgcgggc
     2341 tggcgcgggg cggcagctcg cgcgggaagg ccggcacatg gcaagccggc aacatggtcc
     2401 aggtccactg gtcggctcgt ttgacggatc agctcggcgc cttcaccggc gaactgatcc
     2461 acgcgggcgc ggctcacgcg atgcagcagg cgctatcgct ctctatgctg acctccatct
     2521 gctcggttgc cgaaggcgcg ttgccggaac gcgagccgca tccgggcatc ttcgacggat
     2581 tgctgcggtt gatcccccaa ctgccgcagg gcgaggcgct gctgacggaa gtaatccagt
     2641 gggagatggt cgtgttggcc gacctgggtt atggcctcga cttcgcaatg tgcgccgtga
     2701 ccggccggac ccacggactg gcctatgttt caccgaaaac cggacgagcg gtgacggccg
     2761 agggggcagg ggactgggct tcccgattgc tgccgttgcc aggctttatc actgggacga
     2821 cgccgccaga tctaatcggt tggcgggacg gtctgagact gaccgggcat tttctcgccc
     2881 gcgacgcgtt cggcaatcaa caccgtcccc tgccacaggc ccgagccatg ctgtacgacc
     2941 gggtcgctgc catggtggcc ggcgccgagg cggttgaaca cggctccgtc ggtcgatgat
     3001 gcggtgccct tcattgcccg acaatcccga ccggtttggt caaccaggtc gttggcaatc
     3061 gagcc
//
LOCUS       DASWQN010001106         3158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_107738, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001106 DASWQN010000000
VERSION     DASWQN010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107738"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..608)
                     /locus_tag="VGC82_18850"
                     /pseudo
     CDS             complement(100..608)
                     /locus_tag="VGC82_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003626015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
     gene            complement(882..1808)
                     /locus_tag="VGC82_18855"
     CDS             complement(882..1808)
                     /locus_tag="VGC82_18855"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0425385.1"
                     /translation="MIDRLDAMRVFVTALDKGSLAGAGRTLGRSPAAVTRAIAFLEGH
                     VGAQLLHRTTRKMRLTETGERYASVCQHVLTNLEEAELSAAGDRAAPRGVLTVTAPVM
                     FGTRILRPIVGAFLRSYPAVQIRYLLLDRIVNMIDEGVDVAVRIATLPDSSLIGMPIG
                     DVRRVLCASPDYLARKPLIKAPSDLSEHDCIAVAQLTPNGTWTFPPAPGSRIARTVRV
                     KPRLMVNADEVAVDAAVAGEGIIRIMSYKIDSEVRNGRLVVLLPEDELPRLPVHLIVP
                     EGRLAIAKVRAFVDFTRPRLKSEFASMGRPWP"
     gene            1926..2900
                     /locus_tag="VGC82_18860"
     CDS             1926..2900
                     /locus_tag="VGC82_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0425386.1"
                     /translation="MVYTRLVAGLAGALLTSVVHASTAPARAEVPTAATTTVTHYRTA
                     MVDGVSIFYREAGPADGPVILLLHGFPTSSHMFRNLIPVLADRYHVIAPDYPGFGQSA
                     MPDHTQFAYTFGHYADLIDNFLQQSGQRRYAMYVMDYGAPVGFRLALKHPERVSALIV
                     QNGNAYDEGLKAFWDPIKVYWADGSQPHRDALNFLVDPATTKFQYTDGVGDLSRIDPD
                     NWLHDQVLLDRPGNKDIQLDLFHEYGSNVPLYPKFQAFFRERKPPTLIVWGQNDKIFP
                     ADGAGPYLRDLPNAELHLIDTGHFALEDKLDVMGPLIRNFLERNVAPR"
     gene            2964..>3158
                     /locus_tag="VGC82_18865"
     CDS             2964..>3158
                     /locus_tag="VGC82_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425387.1"
                     /translation="MRHRFFDIAFTPAVQAEQVRRGSRAAYAAASAGKSDGTSADVID
                     KRAFAFIEARDTFYLASVSES"
BASE COUNT          619 a          943 c          944 g          652 t
ORIGIN      
        1 gcttccggta atgctgtgcc tcagcttgcg tgagactttg gccatgaacg ctaggcgaac
       61 gcagcatcaa accggaagct cggccgaacc gcgagcccat tacgtggtgt gcggcgtatc
      121 gtggcttgct tcgtacaaaa accacgcgcg ccgctccgtc tcgtcgatga acacttcgag
      181 caagctcgcg gttgccacgt ccttctgctc atcgatcatt tcgtgagcct cccgcatccg
      241 cttcgccagc gcgccattgt cctcacgtaa ttcggccagc atgtcctgcg gcgtgacata
      301 cggggcatcg ttgtcgagga tccgctgatt gcgcgaaatc tcgccgatag aatgcaatgt
      361 ggactggcct atcttgcgca ctcgttctgc caatacatcg gtcatcgcga agatctggtc
      421 ggattgctca tcaagcatta gatggtagcg cggaagtgag gcccactcat gtgccagtga
      481 aagttcttgg tcttcatgta cagcgcgaaa acatcagcca gcaacccatt cagggccgcg
      541 gcgatgtccc gggctgcgtt ggcgccaaga tcggtcggcg tggcaagcgc gggtggtacg
      601 gcatgcattt gatgtggttt cgccatgctt tggactcctt ccgggataag cgagcggaaa
      661 ataggctgga ctaagcttta tgcggtgtct ttctcatttg ctggttggta gggtgcaggc
      721 gccgcgaatg taaacttggt gccaaccggc tcgcaactcg ggacccttga tggtttgaag
      781 gcgctcaccg cctgcaaact gcgcatccgc ccatacgttc gtggcttgcc acacgaatcg
      841 cagtgtcgtg atcagggcaa cagcgatcgt cggtgcacca tctatggcca cgggcggccc
      901 atgctggcga actctgattt cagccgcggc cgggtgaaat ccacgaaggc tctgaccttg
      961 gctatggcca agcgaccttc cggaacgata aggtgaactg gcagccgcgg gagctcatct
     1021 tcgggcagca ggacaacgag acgtccgttc cgaacctcgc tatcaatctt gtacgacatg
     1081 atgcgaatga tgccttcacc agcgacagcg gcatccacgg cgacctcgtc ggcattcacc
     1141 atcaggcgcg gtttgacgcg caccgtgcgc gcaatacggc tgccgggcgc gggcgggaag
     1201 gtccaggttc catttggggt caactgcgcg actgcaatac agtcatgttc cgaaaggtcg
     1261 ctcggtgcct tgatgagggg tttacgcgcg agataatctg gagaggcaca gagcacgcgc
     1321 cggacgtcgc cgatgggcat cccaatcaga ctggagtcag gcagcgtcgc gatccgaacc
     1381 gccacgtcca cgccttcgtc gatcatgttg acgatgcgat cgagaagcag ataacggatt
     1441 tgaaccgcgg gatagcttcg caaaaacgcg ccaacgatgg ggcgcaggat ccgtgtcccg
     1501 aacatcaccg gtgccgtgac cgtcagcacg ccgcgcggcg cggcgcggtc tccggcagcc
     1561 gacaattccg cttcctcaag gttggtcaat acatgctggc atacggaagc gtaccgttcg
     1621 ccagtttcgg ttagccgcat tttgcgagtt gtccggtgca gcaactgcgc gccgacgtga
     1681 ccttctagga aggcgatcgc tcgggtgacg gcagccggtg aacggcccag ggtgcgtccg
     1741 gccccggcga gacttccctt gtcaagggcg gtgacgaaca cgcgcatggc atccagccgg
     1801 tcgatcattc tttcgtccca cgaaaggata gcttccccac gataggtatt ttggccccgt
     1861 gcgcaacggc ataggcttct tggcgaacaa atgcccgctg gctttcagca ttggggagat
     1921 tgaagatggt ctatacaagg ctggtcgccg gcctggcagg cgcgctcctg acttccgtgg
     1981 ttcatgccag cactgctccg gcccgggcag aggtgcctac ggcggccacg acaactgtca
     2041 ctcactaccg cacggcgatg gttgacggcg tcagcatttt ctaccgtgag gcggggccgg
     2101 ccgatggacc tgtaatactg ctgctgcacg gattcccgac ttcgtcccac atgttccgca
     2161 acctgatccc agtcctggct gatcgctatc atgtgatcgc cccggactac ccgggttttg
     2221 ggcaaagtgc gatgcccgac cacacgcagt tcgcttacac attcggccac tatgcggacc
     2281 tgatcgacaa ttttctgcag cagtcgggcc agcgtcggta cgcgatgtat gtcatggact
     2341 atggcgcacc cgtcggcttt cgcctggcgc tgaaacaccc ggaacgagta agcgcgttga
     2401 tcgtgcagaa cggcaacgcc tatgacgagg gcctcaaggc gttctgggat cctatcaaag
     2461 tctattgggc cgacggctcg caaccccacc gggatgcgct gaattttttg gtggatcccg
     2521 ccaccaccaa gttccagtac actgatggtg tcggggacct gtcgcgtatc gacccggaca
     2581 actggctcca tgaccaggta ctgctggata ggcccggcaa caaagacatc cagttggacc
     2641 tgttccacga gtacggcagc aatgtaccgc tttaccccaa gtttcaggcc ttctttcgcg
     2701 aacgcaagcc gccaacgctg atcgtatggg ggcaaaacga caagatattc cctgcggatg
     2761 gcgctggacc gtatctccga gatttgccga atgccgagct tcacctgatt gatacgggtc
     2821 atttcgcgct ggaagataag cttgatgtca tggggccgct gatccgcaac ttcctcgagc
     2881 gtaatgtcgc accacgttga tgcgcggcga acggtacatt atcttgcccg cccgggcggg
     2941 agcagccgac tgtgaggccc ttcatgagac accgcttctt cgacattgcg ttcacgccgg
     3001 cggttcaggc ggagcaggtg cgccgaggat cccgtgcggc gtatgccgcc gcgtcggcgg
     3061 gcaagagcga cggcacgtcg gcagacgtaa ttgataagcg cgcgtttgcc tttatcgagg
     3121 ctcgcgacac cttctatctc gccagcgtct cggaaagc
//
LOCUS       DASWQN010001107         1875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_372339, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001107 DASWQN010000000
VERSION     DASWQN010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372339"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VGC82_18870"
     CDS             <1..258
                     /locus_tag="VGC82_18870"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0425388.1"
                     /translation="PTGGPHDPHTPGLPTVMVISTTQDPATPYQAGVNLARDINARLL
                     TYEGTQHTAFLQGNTCVDDAGISYLADLKLPAEGIRCKPTS"
     gene            complement(338..>1875)
                     /locus_tag="VGC82_18875"
     CDS             complement(338..>1875)
                     /locus_tag="VGC82_18875"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0425389.1"
                     /translation="GNFHRKVTTSSPEAQQYFDQGMRYLWAFNHDESTRSFAKAAQLD
                     PACAMCYWGVALTVGPNYNVPFMAEPRAKAAWEAVQLAQKAGQRATPVERALVDAVSK
                     RYNGDHPLDPTNEGPVLNAYAEAMKNVAKQFPEDLDVQTLYAEAMMNLKPWKLWTSDG
                     SPAPGTEDIIARLESVMDQNPNHPGANHYYIHALEASPHPEKALVCAQRLRGMMPAAG
                     HLQHMPAHIMQRVGRYEDAAEANRAGAAADAAYYARAKPLDYYTMYTGHNYQFLAFST
                     AMEGRKAETLEAVRKARETIPDDMLLAMPGFDWLISERYSAMVRFGLWDEMLAEAAPN
                     PRLPGLTGGYLFGRAVALTAKGKVDDAKATVAQLDDLSMNTPADYGAGNNSARDMFAI
                     GVLVAKARIADVQGKPEEALAFLRDAVSKEDQTAYDEPSDWFFPVRHILGAELLKAGK
                     VSDAEAVYRKDLALHPDNGWALYGLAQALEVQNKNPEAEKVERQFQQAWSKADVALTT
                     SAF"
BASE COUNT          325 a          589 c          560 g          401 t
ORIGIN      
        1 cccacgggag ggccgcacga tccgcatact cccgggttgc cgacagtcat ggtgatttcc
       61 acgacccagg atccggccac cccttaccaa gccggcgtga acctcgcccg cgacatcaac
      121 gcacggctgc tgacctatga gggcacccaa cacactgcct tcctgcaagg caacacctgt
      181 gtcgatgacg ccggcatcag ctaccttgcc gacctcaagc tgccggctga ggggatccgc
      241 tgcaaaccca ccagctgagt ctccggtcat gtcggactgt tggcaggcgt agctctggcg
      301 ttcggcaagc cgcgatggag cgcgttagtg ccgagcctta gaacgctgac gtagtcagcg
      361 ctacatcggc tttgctccaa gcttgctgga attgtctctc tactttttcc gcttcggggt
      421 ttttgttctg tacttccagg gcctgggcta gcccgtacag cgcccagccg ttgtctggat
      481 gaagtgcaag gtctttacgg taaactgctt cagcgtcact gacctttccg gccttgagta
      541 gttcggcacc aagaatatgt cggaccggaa agaaccagtc ggacggctcg tcatacgccg
      601 tctgatcctc cttgctgacg gcgtcgcgca agaaggccag tgcctcctct ggttttccct
      661 ggacatcggc aatgcgtgcc tttgcgacga gcacgccaat cgcgaacata tcgcgggcag
      721 aattgttgcc ggcgccatag tcggccggcg tgttcattga cagatcgtca agctgggcca
      781 ccgtcgcctt tgcgtcgtcc accttaccct tcgcggtcag cgccacggct ctgccgaaca
      841 ggtaaccgcc ggttagcccg ggcagcctag gattgggcgc agcctcagcc agcatctcgt
      901 cccacaagcc gaagcgaacc atggccgagt agcgttcaga gatgagccag tcgaaacccg
      961 gcattgccag cagcatgtcg tccgggatgg tctcgcgtgc tttgcgcacc gcttccagcg
     1021 tctctgcctt gcggccctcc atcgctgtcg agaaagccag gaactggtag ttgtggccgg
     1081 tgtacatcgt gtagtagtcc agcggcttcg ccctggcgta gtacgctgcg tccgccgcgg
     1141 cgcccgcacg gttggcttcg gcggcatctt cgtaccggcc gacgcgctgc atgatgtgag
     1201 cgggcatgtg ctgcagatgt ccggcggccg gcatcatccc tctcaaacgt tgcgcgcaga
     1261 cgagcgcttt ttcggggtgc ggcgaagctt ccaacgcgtg gatgtaatag tggttggcgc
     1321 ccggatgatt cggattctgg tccatgacgg attccagcct cgctatgatg tcctccgtgc
     1381 ccggagccgg actgccatcc gaagtccaca gtttccacgg cttcaagttc atcatcgcct
     1441 ctgcgtacag agtctgcaca tcgaggtcct caggaaattg ctttgcaacg tttttcatcg
     1501 cctccgcata ggcattgagc acaggccctt cgttggtggg atcgagcggg tggtcgccgt
     1561 tgtagcgttt ggacactgcg tcgaccagcg cccgctcgac cggggtggcg cgctgcccgg
     1621 ctttttgcgc cagttgtacc gcttcccacg ctgccttggc gcggggctca gccatgaatg
     1681 gcacgttgta attaggaccg accgtcaagg ccacgcccca gtagcacatc gcacaagcgg
     1741 ggtcgagttg cgcggccttg gcgaaggaac gtgtggactc gtcgtggtta aaagcccaga
     1801 gatagcgcat gccctggtcg aagtactgtt gcgcctccgg cgacgacgtg gtgaccttgc
     1861 ggtgaaagtt tccca
//
LOCUS       DASWQN010001108         3484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_240158, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001108 DASWQN010000000
VERSION     DASWQN010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240158"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VGC82_18880"
     CDS             complement(<1..580)
                     /locus_tag="VGC82_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0425390.1"
                     /translation="MQLRNVAIIAHVDHGKTTLVDKLLGQSGAFREHQQVTDRALDRN
                     DLEKERGITILAKCASVVWNHTRINIVDTPGHADFGGEVERILNMVDGAIVLVDAAEG
                     VLPQTKFVVGKALARGIHPIVVVNKVDRGDARPDEVHTEVFDLFAALDATEEQLDFPI
                     LYGSAKQGWMSTSPTGAHESTAPLFDMVLAHVP"
     gene            930..1133
                     /gene="rpmI"
                     /locus_tag="VGC82_18885"
     CDS             930..1133
                     /gene="rpmI"
                     /locus_tag="VGC82_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010669172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY0425391.1"
                     /translation="MPKLKTKSSVKKRFKITATGKVLAGPGKKRHNLSARTQKAKRTN
                     RGSQTLTHADGITVKQWAPYGIA"
     gene            1149..1502
                     /gene="rplT"
                     /locus_tag="VGC82_18890"
     CDS             1149..1502
                     /gene="rplT"
                     /locus_tag="VGC82_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018980279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY0425392.1"
                     /translation="MARVKRGTTTHARHKKVLEQSKGFVGRSSTSYRIARERLEKSLQ
                     YAYRDRRVKKREFRGLWIQRINAAVREHGLTYSQFICGLKKVGIEMDRKVLAAIAYDD
                     AASFAAIVQKVQAAL"
     gene            1687..2754
                     /gene="pheS"
                     /locus_tag="VGC82_18895"
     CDS             1687..2754
                     /gene="pheS"
                     /locus_tag="VGC82_18895"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY0425393.1"
                     /translation="MNDDLDVLKTSIDAALADAADLRAWDAVRIAVLGRNGSLTLMLR
                     DLGKTPPEQRKDRGAALNRLKDALTSAIEARKNQLEDAALDARLLSERIDPSLPPRPR
                     ETGLIHPISRTMEEIAAIFGAMGCTVVDGPDVESDWFNFGALNIPPHHPARADHDTFY
                     LPSRDGSSPPVLRTHTSPGQIRTMLSQEPPIRVIVTGRTYRADHDATHSPMFHQCEGL
                     VIDRDMTLGHLKGVLIDFLRTFFAISDLPVRFRSSYFPFTEPSMEVDIGWNRKTGELG
                     RGGDWLEILGSGMVHPRVLANCGIDPREWQGFAWGMGVERVTMLKHGIADLRPFFESD
                     LRWLRHYGFNPLSPTMLHEGV"
     gene            2758..>3484
                     /locus_tag="VGC82_18900"
     CDS             2758..>3484
                     /locus_tag="VGC82_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY0425394.1"
                     /translation="MKFTLSWLKSHLLTDASLDRITDTLTQIGLELEGVENRGAALAA
                     FRIAHVVEAAQHPNADRLRACQVDTGNGIVSVVCGAPNARTGMKAVFAAPGAFIPGSG
                     ITLKVGEIRGVRSAGMLLSAREMGLGDDHAGIIELPADAPVGMSYVSWAGFDDPIIEI
                     GVTPNRGDCFSVRGVARDLAAAGLGTLKPFTPATIPPVFEGGPRWEIAFKEACPWIIG
                     RTIRGIRNSPSPKWLQDRLTFIGL"
BASE COUNT          613 a         1115 c         1125 g          631 t
ORIGIN      
        1 ccggcacgtg cgccaggacc atgtcgaaga ggggggcggt cgattcgtgc gcgccggtcg
       61 gcgaagtgga catccagcct tgctttgccg aaccatagag gatcgggaag tcgagctgct
      121 cttcggtcgc atcgagcgcc gcgaagaggt cgaaaacctc ggtgtggacc tcatccgggc
      181 gggcgtcgcc gcggtccacc ttgttgacca cgacgatcgg gtggatgccg cgtgccagcg
      241 ccttgccgac gacgaacttg gtctgcggca gcacgccctc ggccgcatcg accaggacga
      301 tcgcgccatc gaccatgttc aggatacgct ccacctcgcc gccgaagtcg gcgtggccgg
      361 gggtgtcgac gatgttgata cgggtatggt tccataccac cgaggcgcat ttcgccagga
      421 tggtgatgcc gcgttctttc tccagatcgt tgcggtcaag cgcgcgatcg gttacctgct
      481 gatgctcccg gaacgcgccc gactggccga gaagtttgtc cacgagggtg gtcttcccgt
      541 gatcgacatg ggcgatgata gcgacattac ggagctgcat acggacctgg cttttggatg
      601 gtgcagcgca cacttaggcg ctaaccagcg cggatacgag ggaaaaacgg gccgtttcgt
      661 gggtttctgc catgcttcac gggctggcgt cggtacacgc gggcgaagtc cggccttcgg
      721 tcctgattgc ggccggtcct gattgcggcc ggtcctgatt gcggcgtgtc cgtgctgcgc
      781 ggtctgcggt ccgtgcttgc ctggcgtcgc cggcgtcgct ataccgcgcg gctgtctgcg
      841 agaccgggcc tggaagggca tgcccggccg cgactgctcg ccgattaatc cgatccggat
      901 gggtgagcgt gaaccagaag gagaccaaaa tgcccaaatt gaagaccaag tcgtcggtca
      961 agaagcggtt caagattacc gcgaccggca aggtcctggc cggacccggc aagaagcgcc
     1021 acaacctgag tgcccgcact cagaaagcca aacgcaccaa tcgcggcagc cagacgctga
     1081 cccatgctga cggcataacc gtcaagcagt gggcccccta cggcatcgct taagggagga
     1141 ggcacagcat ggcacgcgtc aagcggggta ccaccaccca cgcccgccac aagaaagtcc
     1201 ttgagcaatc caagggcttc gtcggccgtt cgtccacgag ttaccgcatc gcgcgcgagc
     1261 ggctcgagaa gtcgctgcag tacgcctacc gcgaccgccg ggttaagaag cgcgaatttc
     1321 gtggcctttg gatccagcgc atcaacgcag cggtgcgtga gcatggccta acttattcgc
     1381 agtttatatg cggactgaag aaggtcggga tcgagatgga ccgcaaggtc cttgccgcca
     1441 tcgcgtacga cgatgcggcc tcgttcgcgg ccatcgtgca gaaggttcag gccgccctct
     1501 agcgttcggg cccttcgtgt catggccggc gctggttcgg ccattcacga tcggcagtgt
     1561 gggcagggcc aagccgtggt gaccggtgca ctccgctcac gacatggcag ggtcggccct
     1621 ggtcacgacg agattgacaa agggccgcct gagcagggcg gcccttttcg tgtacgggac
     1681 actaccatga atgacgacct tgacgtttta aagacctcca tcgatgccgc tctggccgac
     1741 gccgccgatc tgcgcgcctg ggacgcagtg cgcatcgcgg tgctcggccg caacggcagc
     1801 ctgactctga tgctgcgcga cctcggcaag acgccgcccg aacagcgcaa ggaccgcggt
     1861 gccgcgctga accggctgaa ggacgcgttg acttcggcta tcgaagcccg gaagaaccag
     1921 cttgaggacg cggcactcga cgcccgcctg ctgtcggagc ggatcgatcc gagcttgccg
     1981 ccccggccga gggaaaccgg cctgatccac ccgatttccc ggaccatgga agaaatcgcc
     2041 gcgatcttcg gcgccatggg ctgcacggtc gtggacgggc cggacgtcga aagcgactgg
     2101 ttcaacttcg gcgccctgaa tattccccct catcatcccg cacgcgctga ccacgacacc
     2161 ttctatctgc catcgcgcga tggcagctcg ccgccggtgc tgcgtacgca tacctcgcca
     2221 ggccagatcc gcaccatgct gtcgcaggaa ccgccgatcc gcgtcatcgt cacgggtcgc
     2281 acttaccgag cggaccatga cgcgacgcat tcgccgatgt tccaccagtg cgagggcctg
     2341 gtgatcgacc gcgacatgac gctggggcac ttgaaaggcg tgctgatcga cttcctgcgc
     2401 acctttttcg ccatctccga tctgccggtg cggttccgtt cgtcctattt cccgttcacc
     2461 gaaccttcga tggaggtcga tatcggctgg aaccgcaaga ccggcgaact cggccggggc
     2521 ggtgactggc tggagatcct gggcagcggc atggtgcatc cgcgtgtact ggccaattgc
     2581 ggcatcgatc cgcgggagtg gcagggcttc gcctggggca tgggtgtgga gcgtgtgacg
     2641 atgctgaagc acggcatcgc cgatctgcgg ccattcttcg agtccgatct tcgctggctg
     2701 cgccattacg gcttcaaccc gctgtctccc accatgctgc acgagggggt ctgagcgatg
     2761 aaattcaccc tttcctggct gaagtcgcat ctcctgacag atgcctcgct cgaccggatc
     2821 accgacacgc tgacgcagat cgggctggaa ctggaaggcg tcgagaatcg tggcgccgcg
     2881 ctcgctgcgt tccgcatcgc acatgtggtc gaggcggcac agcacccgaa cgccgaccgg
     2941 ctgcgcgctt gccaggtcga taccggcaac ggcattgtat cggtggtctg cggggcgccg
     3001 aacgctcgca ctggcatgaa agcggtgttc gcggcgccgg gtgccttcat tcccggctct
     3061 ggcatcaccc tcaaggtcgg ggaaatccgt ggcgtacgaa gcgccgggat gctgctttcg
     3121 gcccgggaaa tgggtcttgg cgacgatcat gccggcatca tcgagctacc ggccgacgca
     3181 ccggtcggca tgtcctatgt cagttgggca ggatttgacg acccgatcat cgaaattggc
     3241 gtcaccccga accgcggcga ctgtttctcg gtccgcggcg tggcccgcga cctggctgcc
     3301 gctggtctgg ggacgctgaa gccgttcacc cccgccacga tcccaccggt gttcgagggc
     3361 ggcccgcgct gggagatcgc cttcaaggag gcctgtccct ggatcatcgg ccgcaccatc
     3421 cgcggcataa ggaactcgcc cagcccgaaa tggctgcagg accggttgac cttcatcggg
     3481 cttc
//
LOCUS       DASWQN010001109         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2616096, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001109 DASWQN010000000
VERSION     DASWQN010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2616096"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VGC82_18905"
     CDS             <1..315
                     /locus_tag="VGC82_18905"
                     /inference="COORDINATES: protein motif:HMM:NF012272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HEY0425395.1"
                     /translation="HLCDHFSRATCEQGRKLMAMIGMAVSSLDRLETIIPAVVDLGQR
                     HTSYGVQPAHYDTVGAALLWTLNQGLGDAFTPAVEAAWKEVYTTLANVMKDHPGGVVQ
                     SG"
     gene            383..1009
                     /locus_tag="VGC82_18910"
     CDS             383..1009
                     /locus_tag="VGC82_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0425396.1"
                     /translation="MGDNTAVSCYHPYMVAFRKHAPARMTLPEFLSWDAADPSGVPWQ
                     LVDGEPVAMAPGSETHAALQGEIGALLRNHLIAQGSPCRLLSQPGIVPRVRANRNFRI
                     PDLGVTCAPPSQGLMVPDPILLIEILSPSNEAQTRANIWAYTTIPSVREILAVHSTRI
                     EAELLRRNGDDSWPEEPDMVTGLGALHLSSIGFTTTLAAFYRTTALAL"
     gene            1085..2764
                     /gene="ettA"
                     /locus_tag="VGC82_18915"
     CDS             1085..2764
                     /gene="ettA"
                     /locus_tag="VGC82_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY0425397.1"
                     /translation="MASYQYVYVLKDLTKAFPGGREVFKGITLSFLPGVKIGVLGVNG
                     AGKSTLMKIMAGIETEYGGEAWAAPGSTVGYLAQEPPLDPTKTVGENVEEAFAELKAA
                     LNRFNEISMLFAEPMSDEEMNALLVEQGDLQEKIDSKDGWELDRKVDIALDALRCPPA
                     DAPVTMLSGGEKRRVALCKLLLEKPDLLLLDEPTNHLDAESVAWLEHTLHDYAGTVMV
                     VTHDRYFLDNVTGWMLELERGRGYPFEGNYSSWLQQKRKRLQQEEKEESARQRALAAE
                     SEWIAASPRARQTKSRARIAKYEEMLQKSQELATGVAEIAIPPGPRLGNVVIEAENLR
                     KGYGNNILIDDLNFKLPPGGIVGVIGPNGAGKTTLFRMITGQEHPDAGSLRIGDTVKL
                     GYVDQSRDSLDPDKSVWQEISGGEEVIYLGKRAVPSRAYVAAFNFKGSDQQKKVGILS
                     GGERNRVHLAKMLKVEHNVLLLDEPTNDLDVDTLRALEEALSEFAGCAVIISHDRWFL
                     DRLATHILAFEGDSHVEWFEGNFQAYEEDKRRRLGADATEPHRIKYRPLAR"
BASE COUNT          615 a          890 c          958 g          529 t
ORIGIN      
        1 catctctgcg accacttttc cagggcgaca tgcgaacagg gccgcaagtt gatggcgatg
       61 atcggtatgg cggtttcaag cctggatagg ctggaaacca tcattcccgc ggtcgtggac
      121 ctcggccagc ggcacacaag ctacggcgtt cagccggcgc attacgacac ggtcggcgcc
      181 gcgctcctct ggaccttgaa tcaagggctg ggggatgcgt tcacgccagc cgtggaggct
      241 gcctggaagg aggtctacac gaccctcgcc aacgtgatga aagaccaccc gggcggcgtc
      301 gttcaatcgg gatgaaactg caccggctcg aagactgttt gccgtcgaac cgtcggtcct
      361 gcggctaacg tgctccaaca cgatgggcga caatacagcc gtgtcgtgct accatccgta
      421 tatggtcgct tttcgcaaac acgcgcccgc ccgcatgacc ttgccggaat tcctgtcttg
      481 ggacgcggcg gatccgtcgg gcgtaccctg gcagcttgtg gatggtgaac ccgtggcgat
      541 ggctccgggt tcggagaccc atgccgcgct gcagggggaa atcggcgcgc tgctccggaa
      601 ccatctgatc gctcaaggca gtccgtgtcg cctgctcagc cagccgggga tcgtgcccag
      661 ggtgcgggcc aaccgcaatt tccggattcc cgatcttgga gtgacctgtg ctcccccatc
      721 tcaggggctg atggtgccgg acccgatcct gttgatcgaa atcctgtccc cgagcaacga
      781 agcgcagact cgcgccaata tctgggccta cacgacgatc cccagcgtgc gggagatcct
      841 ggcggtgcat agcacgcgga tcgaggcgga actgctgcgc cggaacggcg acgatagctg
      901 gccggaagaa ccagacatgg taaccggact gggcgcactc catctatcca gtattggctt
      961 caccacgacc ctggccgcgt tctaccggac cacggccctg gcattgtaaa gccgccgcgt
     1021 tgccctgcta ccagaagggc gtcacgcgcg ttagaaccca ctccgaatag agaagcgacg
     1081 gatcatggcc agctaccagt atgtttatgt gttgaaggat ttgaccaagg ctttccccgg
     1141 agggcgagag gttttcaaag gtatcacgct gtccttcctg ccgggcgtga agatcggcgt
     1201 tctgggcgtg aacggcgccg gcaagtccac gctgatgaag atcatggccg gcatcgagac
     1261 ggaatacggt ggcgaggcct gggccgcgcc gggctccacc gtcgggtatt tggcgcagga
     1321 gccaccgctc gaccccacca agaccgtagg cgaaaatgtc gaggaggctt tcgccgaact
     1381 gaaagccgcg ctcaatcggt tcaacgagat ctccatgctg ttcgccgaac ccatgagcga
     1441 tgaggagatg aacgccctac tcgtcgagca gggcgacctg caggagaaga tcgactccaa
     1501 ggacggctgg gaactcgacc gaaaggtgga tatcgcactg gacgcgctgc gttgcccgcc
     1561 cgccgatgcg ccggttacca tgctgtcggg tggtgagaag cggcgcgttg ccctgtgcaa
     1621 gctgctgctg gaaaagcccg atttgctgct gctggacgaa ccgacgaacc atctggacgc
     1681 cgagagcgtc gcgtggctgg aacacacgct gcatgactat gccggcacgg tgatggtcgt
     1741 cacccacgac cgatacttcc tggacaacgt caccggctgg atgctggaac tggagcgtgg
     1801 ccgcggctat ccgttcgagg gcaattactc gtcatggctg cagcagaagc gcaagcgtct
     1861 gcaacaggaa gagaaagaag agagcgcccg ccaacgcgcc ctggctgccg aatccgaatg
     1921 gatcgcagcg tctcctcgcg cgcgacagac caaaagccga gcgcgaatcg cgaaatatga
     1981 ggagatgctg caaaagtcgc aggagctggc gacaggtgtc gccgaaatcg ccattccacc
     2041 gggtccaagg ctcggcaatg tcgttatcga agccgagaat ttgcgcaaag gctacggcaa
     2101 caacatcctg atcgacgatc tcaacttcaa gttgccgccg ggcggcatcg tcggtgtgat
     2161 cggaccgaac ggcgccggca agacgaccct gttccggatg atcaccggac aggaacatcc
     2221 ggatgccgga tcgctgcgga tcggtgacac ggtgaagctt gggtatgtgg accagtcgcg
     2281 cgacagcctc gatcccgaca agtcggtctg gcaggagatt agcggcggcg aggaggtcat
     2341 ctacctggga aaaagggccg tgccgtcccg cgcctatgtg gctgccttca acttcaaggg
     2401 ttccgaccaa cagaagaagg tcggcattct ctcaggcggt gagcgcaacc gcgtgcatct
     2461 ggcgaagatg ttgaaggtcg agcacaacgt gctgctgctg gacgagccga ccaacgacct
     2521 cgacgtggat acgctgcggg cactggaaga ggcgctgagc gagtttgccg gctgcgcggt
     2581 gatcatcagc catgaccggt ggttcctgga tcgtctggcc actcatatcc tggcgttcga
     2641 gggcgacagc cacgtcgaat ggttcgaggg caacttccag gcgtatgagg aagacaagcg
     2701 gcggcgcctg ggcgccgacg caacggagcc gcaccggatc aagtacaggc cgctggctcg
     2761 gtagggaccg gatcgaacac accgaccgtg cgaacgatgt tctccggtcg gcgccatgtc
     2821 cggcaaccgg caatgcctgc ctcttcgcgg tcgtggcagg cattgtgcgc gaccaggtcg
     2881 catggagcgc ccggtatgga cttacggtgt cgatgaaatg caacatcgtc acgtgatcgc
     2941 cgctcatggc ctttcttggt ggatatctta taccaagtcg cgtaaaagct ga
//
LOCUS       DASWQN010001110         4001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1296140, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001110 DASWQN010000000
VERSION     DASWQN010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296140"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VGC82_18920"
     CDS             complement(<1..1168)
                     /locus_tag="VGC82_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0425398.1"
                     /translation="MYSLPLSHSMPRDDPERRVPVLLPYPFPGPFDYSVPPELDPKPG
                     DIVLVPLNNREEVGIVWDAPADDPVPVHKLKSVVGILDSPPMGESLRRFIDWIAAYTL
                     SPPGNVMSMALRVVSGPMRSVTRYRRAERLPPIGATPAGGTPVGGTPVGGTTARVTPA
                     RQRVLDVLARQEPRLAADLLREAGVSGAVLRGMAEAGLVVAETASPDAPFQRPDPDHQ
                     GPALSAAQQEIGAALREAVAERDFSVTLLDGVTGSGKTEVYLEAVAACLREGRQALVM
                     LPEIALSSQWIERFQRRFGVAPAVWHSDLPSRVRKITWAAVAAGTAQVVVGARSALFL
                     PFPDLGLVVVDEEHEAAFKQQDGVVYNARDMAIVRARLSGCPAIVVSATPSLETV"
     gene            1498..1788
                     /locus_tag="VGC82_18925"
     CDS             1498..1788
                     /locus_tag="VGC82_18925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY0425399.1"
                     /translation="MAVRLVVTINAEQGKGGELARVFRERCLDVLKEPGCQQFEVFQS
                     VLNPDKLALLELWQDQAALDVHAKVNTTRAPLPDGLRAAGGSREDYEYSQTR"
     gene            1798..2874
                     /locus_tag="VGC82_18930"
     CDS             1798..2874
                     /locus_tag="VGC82_18930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0425400.1"
                     /translation="MKVSYFETGRYQAPATMPTTWPMPPGAYDRGVGARLYDDMAERI
                     AFVEQLGFDWFSLSEHHYSPRILTPSPPVAAAYIAARVHTIKVALLGPIVSHSNPVRL
                     AEELAMLDTMAAGRLVVGLLRGTSNEALTYDLNPQESRERTDEGMELILKAWSEPQPF
                     GWQGRHFQYRTVSVWPRPATLPHPPTYALGTSREAGDFAARNHIGLGVSYGPFDVMGK
                     ATGHYRDQCARHGWQPEPDQIIFRANIILAETDDAAQRALEAYPARPPFSLGAGIADA
                     LLELDSRNIAGEARPASINRALPINFVGGPDTIVAQIRQCREATGAGVIDLGFQTPGS
                     SDPGALLSVLELFGRQVLPRVHDI"
     gene            2886..3722
                     /locus_tag="VGC82_18935"
     CDS             2886..3722
                     /locus_tag="VGC82_18935"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0425401.1"
                     /translation="MAKAEEEQRSEATFREGWIEADGFRIRFMAAGDGPPLVHLHGAG
                     GLRITPAHGLLSQSFRVIAFEMPGFGASPGFGASAENLRTPDMPAMADTMAKAAVAVG
                     LDRFNLMGTSFGGKVALWLAARQPELVRALVLEAPAAIRPEGSEPVSGTPEQIARRLY
                     AHPERMPVAPAVDPAQAEQTLALVRRLRGPGRDAALEDRLRDLQTPALIVFGTLDSVI
                     PPEMGRIYKELMPNSHLVFVYDAGHAIAAERPEAYAEVVADFLLRNDAFLIRRTRTVI
                     FP"
BASE COUNT          690 a         1307 c         1334 g          670 t
ORIGIN      
        1 ccaccgtctc caggcttgga gttgccgaaa ccacaatggc cgggcagccg gacagccgtg
       61 cccgcacgat cgccatgtcc cgggcgttgt aaacgacgcc gtcctgttgc ttgaacgccg
      121 cctcgtgctc ctcgtccacc accaccaggc cgagatccgg gaacggcagg aacagggccg
      181 agcgggcgcc gacaaccacc tgagcggtgc cggccgcgac cgccgcccag gtgattttcc
      241 gcacccgcga cggcagatcc gagtgccaga ccgccggcgc cacgccgaag cggcgctgga
      301 agcgctcgat ccattgcgac gacaatgcga tttccggcag catcaccagc gcctggcggc
      361 cttcgcgcag acaggcggcg acggcctcga gatacacttc ggtcttgccc gaaccagtca
      421 cgccgtccag caaggtgact gagaaatccc gctcggctac cgcttcgcgc aatgcggcgc
      481 cgatctcttg ctgcgccgcg gacaacgccg ggccttggtg atcggggtcg gggcgctgga
      541 agggagcgtc gggggaggct gtttcggcga cgaccaggcc tgcctcggcc atgcctcgca
      601 gcacggcgcc gctgacccct gcctcgcgca gcaggtcggc ggccagacga ggctcctggc
      661 gcgccaggac atcgagaacg cgctgccgcg ccggcgttac ccgtgcagtt gtacccccaa
      721 ccggtgtccc cccaaccggt gtccccccag cgggcgtcgc gccaatcggc ggcaaccgtt
      781 cggcgcgccg gtatcgcgtc acggaacgca tcgggccgga cacgacgcgc aacgccatcg
      841 acatgacatt gccgggcggc gacagcgtgt aggcggcgat ccagtcgatg aaccggcgca
      901 gggattcccc catcggaggg ctgtcgagga tccccacgac agatttcagc ttgtgcaccg
      961 gcacgggatc atcggccggc gcatcccaga cgatcccgac ttcttcgcgg ttgttcaatg
     1021 gcaccaggac gatgtcgccc ggcttggggt ccagttcggg cggcacgctg tagtcgaacg
     1081 gacccggaaa cgggtacggc aacagcacgg gaacccggcg ttcgggatcg tcccgcggca
     1141 tcgaatggga aagtggcaag ctatacaaca agcgtcttcg cgaaggattg ggccaagtgg
     1201 cggagcatca cggcttagcg tcggtctgca attaactgcg tcgggtcaag cgggccccag
     1261 tagatccgcg gtcggatcgc gcaccaatcg gagccgttga tcataaccgg gacctttagc
     1321 cttaaaaccg cgccttggca tgagattctt tgccgatgcc gccaatacaa gggcaatcgc
     1381 gccgttggcc gttttcgatc gcggccaccg gcgcggcggc gcctcggctt cgtgcttgac
     1441 gcgtgcgcat gcctggtcac tacttccagg cagaaggcgt aaatgggagg aaatgacatg
     1501 gcggtccggc tcgtcgtcac gatcaatgcg gagcaaggca agggcgggga actcgcccgc
     1561 gtattccggg aacgctgtct ggatgtcctg aaagaacccg gctgccagca gttcgaggtt
     1621 ttccagagcg tgctgaaccc ggacaagctg gcattgctgg agttgtggca ggatcaggca
     1681 gcgctcgatg tccatgccaa ggtcaacacc acccgagccc cgctgccgga tgggcttcgc
     1741 gcggcgggcg gctcgcgcga ggattacgaa tacagccaga cccgttagcg gcccgcgatg
     1801 aaggtttcgt attttgagac cggccgctac caggcgccgg cgacgatgcc gacaacctgg
     1861 ccgatgccgc ccggcgccta tgaccgaggc gtcggtgcca gactatatga cgacatggcc
     1921 gagcgcatcg cgttcgtcga acagctcggt tttgactggt tcagcctgtc cgagcatcac
     1981 tattcgccgc gtatcctgac gccgtcgccg ccggtggccg cggcctatat cgccgcccgc
     2041 gtgcacacga tcaaggttgc cctgctgggc ccgatcgtat cccacagcaa tcccgttcgg
     2101 ctggccgagg aactggcgat gctggatacc atggcggcgg gccgcctggt cgtcgggctg
     2161 ctgcgcggca cgtcgaacga ggcgctgacc tacgacctca atccccagga atcccgggaa
     2221 cggaccgatg aggggatgga gctgatcctc aaggcgtggt ccgagccgca gcctttcgga
     2281 tggcagggcc ggcatttcca gtatcgcacc gtgtcggtct ggcctcgccc tgcgacgctg
     2341 ccccatccgc caacctatgc actggggacg agccgtgagg cgggagactt cgcggcgcgc
     2401 aaccatatcg ggctgggcgt ctcgtacggg ccattcgacg tgatggggaa ggccaccggc
     2461 cattaccggg accagtgtgc ccgccacggc tggcagcccg aacccgacca gatcatcttc
     2521 cgggccaaca tcatcctggc ggaaaccgac gacgcggcgc agcgcgcatt ggaggcctat
     2581 cccgctcggc cgccgttttc cctcggggcg ggcatcgccg atgcgctgct ggagctggat
     2641 agccgaaaca tcgccggtga ggcacgcccc gccagcatca atcgcgcgct gccgatcaac
     2701 ttcgtgggcg gcccggacac catcgtcgcg cagatcaggc aatgccggga ggcgaccggg
     2761 gcaggggtga tcgatttggg tttccagacc cccggttcct ccgatcccgg ggcgcttctg
     2821 agcgtgctgg agctgtttgg caggcaggtc ttgccgcgtg tccacgacat ttagggaccg
     2881 ggatcatggc aaaggccgag gaggaacagc gatcagaggc cacgttccgc gagggatgga
     2941 tcgaagccga cggctttcgg atcaggttca tggcggcagg ggacggtccg ccgctggtcc
     3001 atctgcatgg tgcgggcggc ctgcgcatca cgccggccca tgggctgctt agccagtcat
     3061 ttcgggtcat cgcattcgag atgccgggtt tcggagcctc gccgggtttc ggggcctcgg
     3121 cggagaattt gcggaccccg gacatgccgg cgatggccga cacgatggcg aaagcggctg
     3181 ttgcggttgg gctggaccgc ttcaacctga tgggcacatc gtttgggggc aaggtcgcgc
     3241 tgtggctggc cgcccggcag ccggagctgg tgcgtgcgct ggtcctggaa gcacccgcgg
     3301 cgatacggcc ggaaggttcc gaaccggtat cgggcacgcc cgagcagatc gcgcgccggc
     3361 tttacgcgca tccggaaagg atgccggttg cgcccgcagt ggacccggca caggcggagc
     3421 agacgttggc gctggtgcgg cggctgcgcg gtccgggacg cgatgcggcg ctggaggatc
     3481 ggcttcgcga ccttcaaacc ccggctttga tcgtattcgg cacgctcgac agcgtcatcc
     3541 cgccggagat ggggcggatc tacaaggaac tgatgcccaa ctcgcacctg gtgttcgttt
     3601 acgacgccgg ccacgccatt gcggcggagc gccccgaggc gtacgcggaa gtcgttgccg
     3661 atttcctgct acgcaacgac gcgttcctga tcaggcgcac ccggacggtg attttcccat
     3721 agctgaacga caagcggttc acagcgagcc gagcacggca gcgggctatt tcgatattgc
     3781 tacgtcttaa cttacagacc cgcatgagtc atgctcacaa gcttgtcgct ccagtcggcg
     3841 gccagcgccc aagcgcaacg aagtgcaccc tgccctcgat cagggtcttt ttgattgtgg
     3901 gattgacccg ctttggctca cgcgccgacc gccatgcttg tcacccatct tttggcgttc
     3961 aggggcggcg aaacgctccg tcgcgttccg gtcagccgaa c
//
LOCUS       DASWQN010001111         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1162845, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001111 DASWQN010000000
VERSION     DASWQN010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162845"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VGC82_18940"
     CDS             <1..261
                     /locus_tag="VGC82_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425402.1"
                     /translation="QIIDAVTAQALHADACRDHPVVGWVVMRDQPEPGAFIARLVTGV
                     PTPYVLRADSLAEIHAQLPPGLVRSERQPAEPPDLAEIWFAQ"
     gene            486..1193
                     /locus_tag="VGC82_18945"
     CDS             486..1193
                     /locus_tag="VGC82_18945"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0425403.1"
                     /translation="MTSSQFASRTASPTAPSYEGFTARRIELALWEAIVSLEIRPGTR
                     LSEQEIADHYRVSRQPVRQALIGLASKNLVVVLPQRGTIVTRLSTKRLMEARFVREAI
                     ETAVVRQACTDFLPASRAYLDTVLAAQAKAAATDDPGAFAQEDQEFHLTLANGIGCPL
                     AWVGIRDLKAHMDRVTRLILRGTPWMTFLVQQHTNIVEAIDRRNPDAAVTAMSDHMAA
                     VVQQLPSAQAEHPDLFD"
     gene            1224..2108
                     /locus_tag="VGC82_18950"
     CDS             1224..2108
                     /locus_tag="VGC82_18950"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0425404.1"
                     /translation="MQPYQIAAGLTVTPLHPLIGAEIRGVNLAAPVDGRTLLAIHQAW
                     LRHMLLVFPDQRITDAEQITFARQFGDLEIHPAREHRSSLHPEIFRVSNVDEEGNIIP
                     SDGNAWGYINITWLWHSDSSFRQVPSKGSILHGIEVATEGGRTMFCNLYAVYEALPAE
                     LKQRIHGRRAIHSHDAVLANNPRLKQGDQYEDLRAIHPLVRRHPDTGRQFLFLSPHTM
                     SGIEDMPEAEARVLLDKLARFATQEQFVYAHAWRQDDVIMWDNRCTMHAVMPYDNKTV
                     RRIMHRTTLVGESPVLAA"
     gene            2126..2968
                     /locus_tag="VGC82_18955"
     CDS             2126..2968
                     /locus_tag="VGC82_18955"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0425405.1"
                     /translation="MTKVLSATDIAAYRRDGVCFPIPALTPREALHYRTCLEAFERSQ
                     GAPLRGSLMFKTHLLFTWVDELIRHPKVLDAVEDILGPDLLAWNTHWFIKEPGDGRYV
                     GWHQDLTYWHLEPDEAITAWIALSPVTVESGAMRMVPGTHTREVVEHTDTWRQGAMLT
                     RGQEIAVEVDAAAAVDVELQLGQMSLHHHKIFHSSPANQANDRRIALAVRYIPTHVRQ
                     VAINDDSAALVRGTDRYGHFRHEPRPLRDMDPDLVPQQQAAAARQANILYAGTGRTSL
                     RADL"
BASE COUNT          683 a         1204 c          985 g          635 t
ORIGIN      
        1 cagatcatcg atgcggttac ggcccaggcg cttcatgccg atgcctgccg cgatcatccg
       61 gttgtgggct gggtggtgat gcgcgaccag cccgaacccg gcgcgttcat cgcccgtctg
      121 gtgaccggcg tgcccacgcc ctacgtgctg cgggctgaca gcctcgctga gatccacgcc
      181 caactgccgc cgggtctggt gcgctcggag cgccaacccg cggaaccgcc ggatttagcc
      241 gagatctggt tcgcgcaata gcgcggctgc ggatatccgg ctccggcgag atctccggtt
      301 acagctggtg acacgactgc tgggttttac cccaagtggg cggggcacgg caggggcgga
      361 ctgtcccggc atcgccaggt gcggcttatg ccttgcggtc gcttctgctg ctttttggct
      421 ctggcagcct ccacggcaac ggcaccgtgg ttgcgcaaaa acccactgca tactaggatc
      481 ctagtatgac aagtagtcag ttcgcctctc gcaccgccag tcccaccgct ccctcctacg
      541 aaggcttcac cgcccgccgc atcgaactcg cgctttggga ggcaatcgtt tcactggaaa
      601 tccgacccgg cacacgcctc tcggagcagg aaatcgccga ccattaccgc gtctcccgcc
      661 agcccgtccg ccaagccctg atcggcctcg ccagcaagaa cctcgtcgtc gtcctgcccc
      721 agcgcggcac catcgtcacc cgcctctcaa ccaagcgcct gatggaggcg cgtttcgtcc
      781 gcgaggcaat agaaaccgcc gtggtccgcc aagcctgcac ggacttcctc ccggcaagcc
      841 gggcctacct ggatacagtg ctggccgcac aggccaaggc agccgcgacg gacgacccag
      901 gcgccttcgc acaggaggac caggaatttc accttaccct cgccaatggc attggctgcc
      961 cgcttgcctg ggtcggcatc cgcgacctca aggcccacat ggatcgtgtg acgcgcctga
     1021 tcctgcgtgg caccccctgg atgacgttcc tcgtccagca acacaccaac atcgtcgagg
     1081 cgatcgaccg ccgcaatccg gacgccgccg ttacggcgat gagcgaccac atggctgccg
     1141 tcgtccaaca actgccaagc gcccaggcgg agcatcccga cttgttcgac taaaaatgca
     1201 atgacgaaac gaagaggcaa cacatgcagc cataccaaat cgccgccggc ctgacggtaa
     1261 cacctttgca cccgctcatc ggtgccgaaa ttcgcggcgt caatctcgcc gcgccggttg
     1321 atggccgcac gctcctcgcc atccatcagg cctggctacg acacatgctg ctggtgttcc
     1381 ccgatcagcg catcactgac gccgagcaga tcaccttcgc ccggcagttc ggcgatctcg
     1441 aaatccaccc cgcccgagaa caccggtcca gcctccaccc cgaaatattc cgagtgtcta
     1501 acgtcgacga agagggaaac atcatcccgt cggacggcaa tgcctggggc tatatcaata
     1561 tcacctggct gtggcattcc gacagttcgt tccgccaagt gcccagcaaa ggctcaatcc
     1621 tgcacggcat cgaggtcgcc accgaaggcg gcaggacgat gttctgcaac ctgtacgccg
     1681 tatacgaggc gctgcccgcg gagctgaagc agcgcatcca cggacgccgt gccatccaca
     1741 gccacgatgc cgtcctggca aacaacccca ggctcaagca aggcgatcag tacgaggacc
     1801 tgcgcgccat acatccgctc gtgcggcgcc accccgacac cggccggcag ttcctgttcc
     1861 tcagccccca tacaatgtcg ggcatcgaag acatgccaga ggccgaggcc cgcgttctgc
     1921 tcgacaagct cgctcggttc gcaacgcagg aacagttcgt ttacgcccac gcctggcgtc
     1981 aggacgatgt catcatgtgg gacaaccgat gcacgatgca cgcggtgatg ccctacgaca
     2041 acaagaccgt gcggcggatc atgcaccgca ccacgctggt cggcgaaagc ccggtcctgg
     2101 ccgcgtgacc accaaggagg aaacaatgac caaggtctta tctgccaccg acatcgccgc
     2161 ctatcgacgc gatggcgtat gcttcccgat ccccgcgctc accccccgcg aggctctgca
     2221 ctaccgcact tgcctggaag cgttcgaacg cagtcaaggc gccccactgc gcggcagcct
     2281 gatgttcaag actcatctcc tgttcacctg ggtggacgag ctgatccgcc atccgaaggt
     2341 gctggacgcc gtcgaagaca ttctcggccc tgacctgctc gcctggaaca cgcactggtt
     2401 catcaaggaa ccaggcgacg ggcgctatgt cggctggcac caggatctga cctattggca
     2461 tctggaaccg gacgaggcga tcaccgcctg gatcgcgctg tccccggtaa ccgtcgaaag
     2521 cggcgcaatg cgaatggtcc caggaacgca cacacgcgag gtcgtggagc acaccgatac
     2581 ctggcgtcag ggggcaatgc tcacccgtgg ccaggaaatt gctgtcgagg tggacgcggc
     2641 agccgcggtc gacgtggagc tgcagctcgg acaaatgtcg ctacaccacc acaagatctt
     2701 ccactcgtcg ccagccaacc aggcgaacga ccggcgcatc gccctcgcgg tgcgctacat
     2761 cccgacccat gtccggcaag tggcaatcaa tgacgacagt gccgccctgg tgcgcggcac
     2821 cgaccgttac gggcatttcc gccacgagcc tcggcctctg cgcgacatgg atcccgacct
     2881 cgtaccgcag cagcaggccg ccgctgctcg gcaagcgaac atcctctacg ccggcaccgg
     2941 tcgcaccagc ctgcgcgccg atctttaatc ccagcaagcc cggatccgca gcgcccatct
     3001 cgacccgacg ggcttcaaac gggcccggct gcggatggcg ccgatgcccg tgcccgctgg
     3061 catagggctc ggctctgtca cacaggcgtg aagcttggca accatctgcg acaggaatgg
     3121 tcttcttctt gaacagaagg ataccccttg tgctgctgct tgcgatttgt ctatgtggca
     3181 ctgccttgag ctactatctc ggcctgcgaa atcccggcct tcgaggaggt ggcacgacca
     3241 gaggatggtt gctgtcctta gtgatcggct gcgtattggc ggccgtattt tacggatttt
     3301 ccactctggg actgattgtg tcactcgata cgctgctttc agatttttaa aagttcaggc
     3361 tgatcccgtt gagcgccgta tcctgcgttt ttgggcagcc gcctcgttct actgtgtcac
     3421 aacacgcagt gcatattgaa atcattgcct tttctagaac cgaggttatt cagaaataca
     3481 gcaatttcaa tgcgttgaga tttttct
//
LOCUS       DASWQN010001112         6315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2087913, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001112 DASWQN010000000
VERSION     DASWQN010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087913"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..976)
                     /locus_tag="VGC82_18960"
     CDS             complement(join(37..642,644..976))
                     /locus_tag="VGC82_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611132.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY0425406.1"
                     /translation="MPRPCSSDLRERVIEAVEEGASRREAAERFEVGASSAVRWFQAW
                     QKEGRRAPRPCGGSRSPLEDHADQILALVAEQPDWTLDEFVAALRKRRLAGSRSALWR
                     FLERHGISVKKSLRAAEQDRADVARARRRWIRQQGLLDSSALVFIDETAANTSLARLY
                     GRCPRGERLIGRVPFGTWETLTFVAGLRRDGMTAPLVIEGAMNGATFLSYVEQCLAPA
                     LNRGDIVVMDNLRAHKVAGVKEAIEAAGAQLRYLPEYSPDLNPIEMSFSKLKAYLRKA
                     AARTQRSLRRRIGLFVPRLGADECANYFAHAGYVSI"
     gene            complement(1118..1975)
                     /locus_tag="VGC82_18965"
                     /pseudo
     CDS             complement(1118..1975)
                     /locus_tag="VGC82_18965"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
     gene            complement(1912..2796)
                     /locus_tag="VGC82_18970"
     CDS             complement(1912..2796)
                     /locus_tag="VGC82_18970"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEY0425407.1"
                     /translation="MAFKVQVGPPQIAIHQAMTVLVTESDGQINSPSDKGLYFFDTRL
                     ISAWSVYANGMPWDLLNGGALNFDASRIFLTNQAFLTEDGAIARQTLAFSLSRVMDQG
                     LHEDLDITNHGQKPVRFNLEIALRSDFADVFDVKAKRDIRRGHISTDWSEPRQTLRTT
                     YINADFSRSVAVSVGRSDSRAVFANGRLSFEVRLPPGGTWHCCLLYEFADGDKRFHAP
                     HECALHAETSPQAIAHRDWRQTVLKLRTSNEEFYRFYQQAIDDMAALRLPIAAPITWC
                     SCPPRGCRGSWRRSAATA"
     gene            3286..4245
                     /locus_tag="VGC82_18975"
     CDS             3286..4245
                     /locus_tag="VGC82_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY0425408.1"
                     /translation="MTDLPSSVEIHEEGPREGFQIEPGPIPTANKIRLIEALAETGLH
                     HVQACSFVNTRLVPGWADAEAVVEGFAAKPDVEYTGLFFNGNGLDRALAFKGKLTIRG
                     SISLTASEGFTRKNLNRSHAENVEAMKKHSAAQLAKGVPVNRVGVMAAFGCNYQGDIT
                     PAQVISTLQDGLAIAEETGAEITLFSLADTMGWATPARIERVIGEVRGRWPNMELSLH
                     LHDTRGLAVANAHAGLKMGVTHYDSTVGGLGGCPFAGQPKAAGNICTEELVLLCEEMG
                     IETGVDLDALIEVGRLAEEIVGHQLPSELIHAGSLDAFRRKAA"
     gene            complement(4411..4719)
                     /locus_tag="VGC82_18980"
     CDS             complement(4411..4719)
                     /locus_tag="VGC82_18980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY0425409.1"
                     /translation="MQTKHRDQRLAFTVTWEVREGKLDAAADIIARFAPEARKEPGLE
                     LLAVNQSTTNPAQFLLYEVFKDAEAFEAHQQTPHFRTLILEEALPLLSKRERVQYAPL
                     "
     gene            complement(4923..6305)
                     /gene="aceA"
                     /locus_tag="VGC82_18985"
     CDS             complement(4923..6305)
                     /gene="aceA"
                     /locus_tag="VGC82_18985"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEY0425410.1"
                     /translation="MRQTPAPCFAPDGSYGGDRPEPGRFDGIRRDYSPADVARLSGSF
                     RIEHTLADMGARRLWQLLKTEPQVTTLGALTGMQALQQVKAGLKAIYLSGWQVAADAN
                     TAGTMYPDQSLYPVDSVPKVVRNINNALRRCDQIAQSEGGKDGTYWMAPIIADAEAGF
                     GGPLNAYELMRNMIASGAAGVHFEDQLASEKKCGHLGGKVLVPISQHIRTLNAARLAA
                     DVEGVNTILLCRTDAQSAQLLTSDVDERDRPFIGNDRTAEGFFRIRPGCGNNYAIARG
                     LAFAPYSDLLWWETSEPDLREAERFANEIHKQFPGKMLAYNCSPSFNWKKKLPADKIA
                     AFQREIGKMGYRFQFVTLAGFHSLNLSMFNLARGYRERGMAAYSELQQAEFAAEPEGY
                     TATRHQREVGVGYFDAVAMAISGGKSSTTAFAGSTEADQFHDHGDVVQAHDRHDDGLV
                     HNHDWAVTAK"
BASE COUNT         1158 a         2032 c         1987 g         1138 t
ORIGIN      
        1 caagtgttag attcgatcca ctagagcgga ttccgttcat attgaaacat atccggcatg
       61 agcgaagtag ttcgcgcatt cgtctgcgcc gaggcgcggc acgaaaaggc cgattcggcg
      121 gcgcaggctc cgctgggtcc gcgcggcggc tttgcgcaga tacgccttga gtttgctgaa
      181 ggacatctcg atcggattga gatcgggcga gtactcgggc aggtatcgca attgtgcacc
      241 ggcggcctcg atcgcttcct tgacgccggc gaccttgtgg gccctgagat tgtccatgac
      301 gacgatatcg ccgcggttga gcgcgggagc caagcattgc tcgacgtaag acagaaaggt
      361 ggctccgttc atggcccctt cgatcaccaa gggcgccgtc attccgtcgc ggcgcaggcc
      421 ggccacgaag gtcagcgtct cccaggttcc aaacggcacc cgcccgatca atcgctcgcc
      481 ccgcgggcat cggccgtaaa gacgagccag gctggtgttc gccgcggttt cgtcgatgaa
      541 gacaagggcg gacgaatcaa gcaggccctg ctgccggatc cagcgccgcc gagcgcgggc
      601 cacgtccgcc cggtcttgct cggctgcccg caggcttttt tttgacgctg atgccgtggc
      661 gctccagaaa ccgccaaagc gcgctgcggc tgccggcaag ccggcgcttg cgcagggccg
      721 cgacgaactc gtcgagcgtc cagtccggct gctcagcgac cagagccagg atctggtccg
      781 cgtggtcctc cagcggcgac cggctccccc cgcacggcct tggcgcgcgt ctgccctcct
      841 tctgccaggc ctgaaaccac ctgaccgccg agctggcacc cacctcaaaa cgttccgccg
      901 cctctcgccg cgatgctccc tcttccaccg cctcgatcac acgctcccgc aaatccgacg
      961 aacaaggtct cggcatggcg cacccccaga agaccgaatc accccccaag actctacctt
     1021 tccgcagatc atgggaatcc cgattcaacc ccgacggaac ctgctctagt gcgatgatcg
     1081 cattgtcgtg cggccatacc gaaccgttct ggtaggagta cgggttgtat gcggggtgct
     1141 tgctggacag cgtgcgaatg ccccagccgg aactcatgtc cggcgccatc aaccgggcga
     1201 cgacccgggc cgcgcgatcc gccggcacga tgcccgacca aagcaagtgc cccggattgg
     1261 aggcgacggt catcaccttc cgcttctcgc catccagcat ataggcgtag aacccggtcc
     1321 cttcgtccca gaacgcggcg ttgaaacgat caaacagatc ggcggctttc cggcgtaacg
     1381 tcgccgcgcg atcgggctta ttcagagcgt cgaacacctc ggccatgcgc acccacgcgt
     1441 catacacgta gccttgcagt tcacacagcg cctttggtcc gcgcaccggc gagccgtccg
     1501 gataaaccat cgaatcgccg gcatctttcc aggccacgtt ctcgtagccc acgggcgaac
     1561 gggtctggta ctcctgcaac atgtcgccgt cgcggtcgcc gtaattgtcg atccagtcca
     1621 atgccgcttc ggcgttcggc agatatcgtt ccagcagcga atgatcgccg gtcgcccgcc
     1681 acgcggcatg cagcgtcacc agatacagtg gcgtcgcgtc ggcggtgccg tagtacggcg
     1741 tgtgcggaat cagcttgaaa tgcgcgagct cgccgtatcg cagttcgtgc atgatcttgc
     1801 caggttcggc gtcgcggaaa tcgtcgcgtt ccttgctttg ccaccgtccc aggacatcca
     1861 gcgagcccct ggcgaattcc ggatagacaa gaatgttttg cagcgaaacg atcaggctgt
     1921 cgcggccgaa cggcgccatg aaccacggca accccgcggc gggcaggaac accatgtgat
     1981 cggtgccgcg atcggcagac gcaacgccgc catgtcatcg attgcctgct ggtagaaacg
     2041 atagaactct tcgttgctgg tacgcagctt caataccgtt tgccgccagt cacggtgcgc
     2101 gatggcctgc ggcgaggtct cggcgtgcag cgcgcattca tgaggcgcgt ggaaccgctt
     2161 gtcgccgtcg gcgaactcgt aaagcaggca gcaatgccag gtgccgccgg gcggcaaccg
     2221 tacctcgaaa ctcagccgcc cgttggcgaa cacggcgcgt gaatcgctgc gacccaccga
     2281 gaccgcgacg gaccgcgaga aatcagcgtt gatataggtc gtgcgcagcg tttgccgagg
     2341 ttccgaccag tcggtgctga tgtgcccgcg gcggatatcg cgcttcgcct ttacgtcgaa
     2401 gacgtcggcg aaatccgaac ggagcgcgat ttccaggttg aatcgcaccg gcttctggcc
     2461 gtggttggtg atgtccaggt cctcgtgcag gccctgatcc atgacgcgcg acaggctgaa
     2521 cgccagggtt tggcgggcga tcgccccgtc ttccgtcagg aacgcctgat tggtgaggaa
     2581 gatgcgcgac gcgtcgaagt tcagggcacc gccgttaagc aaatcccacg gcatgccgtt
     2641 cgcgtaaacc gaccaggcgc tgatcagccg tgtgtcgaag aaatacagcc ctttgtcgct
     2701 gggcgaattg atttgcccat ccgactccgt aaccaggacg gtcatcgcct ggtggatggc
     2761 aatttggggc gggccgacct ggaccttgaa cgccatgcaa gaaactccgc gtagtggtgt
     2821 aagccgcgtg ccttatatcc cggaacatgc ggcggagcac ggacaggatg cgcgatctgg
     2881 gcgcgcggga tgctgaatca acctcgcatc caccagcggg aatagcgaga aattggccat
     2941 tggcacgggc tttcgccgcg gcagatcccg cctgcgggaa aaaggcacat gattggtgcg
     3001 aacaagtaag ggaccacggc gtccggggcc accgtcgcac caggaggcca ctggcgcatc
     3061 aggaggccac tggcgcatca ggaatcccgt caccgcgcgc gaagcactgc cagtgccgct
     3121 ccggcggccg cggcaggaaa gtcccgtacg ccgggtcagc cgcttgcaac aaacgcggca
     3181 ccggatgaag atacccggca tgtaggcgat cgacatcgca cgatcctgat cgctggccat
     3241 tccagccgat cgcgggcgcg gcccgataac cgaggacacc cagtcatgac cgacttgccc
     3301 agttctgtag aaattcacga ggaaggcccg cgggagggtt tccagatcga accaggcccg
     3361 attcccaccg ccaacaagat ccgcctgatc gaggcattgg cggaaaccgg attgcatcac
     3421 gtgcaggcct gttcctttgt caatacgcgc ctggttcccg gctgggcgga cgccgaggcc
     3481 gtcgtcgaag gctttgccgc gaagccggac gtcgagtaca ccggcctgtt tttcaacggc
     3541 aacggcctcg accgggcgct ggcgttcaag ggcaagctga cgatccgcgg cagcatctcg
     3601 ttgaccgctt cggaaggctt cacccgcaag aacctcaacc gcagccatgc cgaaaatgtc
     3661 gaggcgatga aaaagcacag tgcggcgcag ctggccaagg gcgtcccggt caaccgggtt
     3721 ggtgtgatgg cggcgttcgg ctgcaactac cagggcgata tcacgccggc gcaggtgatc
     3781 agcaccttgc aggacggcct ggcgatcgcc gaggaaaccg gcgcggaaat aacgctgttt
     3841 tcgcttgccg acacgatggg ctgggcaacc ccggctcgga tcgaacgcgt gatcggcgaa
     3901 gtgcgcggcc ggtggccgaa catggagctg tcgctgcatc tgcacgatac gcgcgggctt
     3961 gcagtggcga acgctcatgc ggggctaaaa atgggcgtga cccactacga ctcgaccgtg
     4021 ggtggcttgg gtggttgccc gttcgccggt cagccgaaag ccgccggcaa catctgcacc
     4081 gaagaattgg tcctgctgtg cgaggaaatg ggcatcgaaa ccggcgtcga tctggatgcg
     4141 ctgatcgagg tcgggcggct ggccgaggag atcgtcgggc accagttgcc gagcgagtta
     4201 atccacgccg gcagcctgga tgcgttccgg cggaaggcgg cctgacgcgc cgggagcaac
     4261 ctgctttcca gattatgcct ggggtgattg gctctcctta ccgccgatgc ctcgcatggc
     4321 cacgctgccg tattcctgca agcacggcca ttcgcagctt ggtgccgagg tcgatcgacc
     4381 ctgttcgagg gccgatcgaa gcacgggtgg ttacagtgga gcgtactgca cgcgctcacg
     4441 cttgctgagc agcggcagcg cttcctccag gatcagcgtc ctgaaatgcg gcgtctgctg
     4501 atgggcctcg aaggcctccg catccttgaa aacctcgtaa agtagaaact gcgcagggtt
     4561 ggtcgtactc tggtttaccg ccagcaattc cagtccgggc tccttgcgcg cttcaggggc
     4621 gaagcgggcg atgatgtcgg cggcagcgtc caacttgccc tcgcggactt cccacgtcac
     4681 ggtaaatgct agccgttggt cacgatgttt tgtttgcacg gtgatctcct gttctgatga
     4741 acagagctga cttaacccca ccgcccgcgc tccgcatcgt ttaacgcttc ggcccggatc
     4801 gaagcgatcc gcagcacttc gttgacatca agagcgccat gttgcgccgc caatcagcga
     4861 acagaaaaag ggcggcgaag ccttcgcccc gccgcccttg ctgaaccttt ctggctaaag
     4921 gctcacttcg cggtcacggc ccagtcatgg ttgtgcacca ggccatcgtc atgacggtca
     4981 tgcgcctgaa ccacgtcgcc gtgatcgtgg aactgatccg cctcggtcga tccggcgaac
     5041 gccgtggtgg acgacttccc acccgagatc gccatcgcca ccgcgtcaaa atatcccaca
     5101 ccaacttccc gttgatgccg ggtcgcggtg tatccttccg gttccgcggc gaattctgcc
     5161 tgttgcagct cgctgtaggc cgccatgccg cgctcccgat agccgcgcgc cagattgaac
     5221 atcgacaggt tcaagctgtg gaaccctgcc agcgtcacga actggaagcg gtagcccatc
     5281 ttgccgatct cgcgctggaa cgccgcgatc ttgtccgccg gcagtttctt cttccagttg
     5341 aagctgggcg agcagttgta agccagcatc ttgcccggga actgcttgtg gatctcgttg
     5401 gcgaaccgct ccgcctcacg caggtcgggc tcgctggttt cccaccacag caggtcggaa
     5461 tacggcgcga aggccaggcc gcgggcgatg gcatagttgt tgccgcagcc cggcctgatc
     5521 cggaagaagc cctcggcggt gcggtcgttg ccgatgaacg ggcgatcgcg ctcgtccacg
     5581 tccgaggtca gcaactgcgc cgactgggcg tcggtgcggc acaacaggat cgtgttcacg
     5641 ccttccacat cggcggccaa acgagccgca ttcagcgtgc ggatgtgctg gctgatcggc
     5701 accagcacct tgccgccgag atggccgcat ttcttttcgg acgccaactg atcctcgaaa
     5761 tgcacgcccg ccgcgccgga tgcgatcatg ttgcgcatca gctcgtaggc attcagcggg
     5821 ccgccgaagc cggcttccgc atcggcgatg atcggcgcca tccagtaggt gccgtccttc
     5881 ccgccctcgg attgtgcgat ctggtcgcaa cggcgcagtg cgttgttgat gttgcgcacc
     5941 actttcggca ccgagtccac cggatacagc gactggtcgg ggtacatggt gccggcggta
     6001 ttggcatcgg ccgccacctg ccagccggag aggtagatcg ccttcagccc ggccttcacc
     6061 tgctgcagcg cctgcatgcc ggtcagggcg ccgagggtgg tcacctgcgg ctcggtcttc
     6121 agcagctgcc acaggcggcg ggcgcccata tcggccaagg tgtgctcgat ccggaagctg
     6181 ccggagaggc gggcgacgtc ggcgggcgaa tagtcgcggc ggatgccgtc gaagcggcct
     6241 ggctcgggac ggtcgccccc atagctgcca tcgggcgcaa agcagggggc aggggtctgg
     6301 cgcatcgggt gaact
//
LOCUS       DASWQN010001113        11146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_503644, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001113 DASWQN010000000
VERSION     DASWQN010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 11146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11146
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503644"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            505..774
                     /locus_tag="VGC82_18990"
     CDS             505..774
                     /locus_tag="VGC82_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425411.1"
                     /translation="MDALEQTTMRKVGRRLIPFLMVCYFFNFLDRVNVSFAALEMNKD
                     LGFSASAFGLGAGILFFTYMLCETPSWSKSARVSGSRASCSPGAS"
     gene            753..1793
                     /locus_tag="VGC82_18995"
     CDS             753..1793
                     /locus_tag="VGC82_18995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425412.1"
                     /translation="MFTWGIIAAGMAFIAGEKSFLTMRALLGAAEAGFFPGIIYYLTL
                     WFPGTYRARVTGLFMACIPLSAAIGAPISTSLLYLDGALGFRGWQWMFLLEGLPSIAL
                     GVATYFFLTGTPTEAKWLGAEERGWLDARIKAEGARKAGALSLSMLETLMNGRVLALS
                     IVAFFIASQLLGVGFFLPTIVKGFGLTNMQTGLVSIIPPACGSVAMIWWGRHSDRLME
                     RRYHLASALIVGAVGIIAAGFIDDPVLKMLAFTISAIGLNASLPVFWTLAPAFLTGAS
                     AAVGIAFINSVAALGAFLAPFMLGLVKDATGSFTGGLYLLGGGVIIAALFVLAFAHAH
                     DLEREPQVQPAG"
     gene            complement(1861..2247)
                     /locus_tag="VGC82_19000"
     CDS             complement(1861..2247)
                     /locus_tag="VGC82_19000"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0425413.1"
                     /translation="MRTDSLDWVGLAGRFLMSAIFIQGGIMKAMAPAATMGYFAKIPL
                     PMPGAAYALTLLIEIGAGILFLVGFRARLTGLVLAAWCIATAMVAHYHPESREQMINF
                     MKNVCMAGGFLQVVAFGAGRLSADRR"
     gene            complement(2244..2666)
                     /gene="ndk"
                     /locus_tag="VGC82_19005"
     CDS             complement(2244..2666)
                     /gene="ndk"
                     /locus_tag="VGC82_19005"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY0425414.1"
                     /translation="MATERTFSIIKPDATRRNLTGKVNAVLEAAGLRIIAQKRIHMTQ
                     AQAETFYGVHAARPFFRDLVTFMISGPVVVQVLEGENAVARNRDLMGATDPKKADPGT
                     IRALFAESIEANSVHGSDSVENAAAEIAYFFAGTELVG"
     gene            complement(2757..3917)
                     /locus_tag="VGC82_19010"
     CDS             complement(2757..3917)
                     /locus_tag="VGC82_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY0425415.1"
                     /translation="MFTRPPLAPYAVSPATARGRLHHEPEAETRSPWQRDRDRIIHSS
                     AFRKLQYKTQVFVNHEGDFYRTRLTHSIEVAQIARSIARALGLDEDLTEALALAHDLG
                     HPPFGHAGEDALNVVMKPFGGFDHNAQSLRIVTLLESRYAGWDGLNLTWETLEGLVKH
                     NGPLRHPARYIAEYNDHHKLDLHTQPAAEAQVAAIADDVAYHTHDLDDGLRARLFTVD
                     DVKHLPLVGASLAAARMSSLDVPPPRLRHEMLRRVINDMVTDLITETGKRLAKLDPDS
                     PDAIRRHKQRVVGFSPAMADANQAIKDFLFARMYRHWRVNRMSTKAMRLTEDLFRLLH
                     TDPTLLPDDWRARAGEGGMQRSAATVADYVAGMTDRYAMDEHRRLTDISING"
     gene            4098..4433
                     /gene="erpA"
                     /locus_tag="VGC82_19015"
     CDS             4098..4433
                     /gene="erpA"
                     /locus_tag="VGC82_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY0425416.1"
                     /translation="MEQFAVTDRAAARIAEIVAATKPAADTALRVAVLAGGCSGFQYR
                     FELDPQTQPDDLVIEHGPARVVVDAVSLDLLSGSELDYTDELMGSHFAVRNPNAKSAC
                     GCGTSFSID"
     gene            4573..5814
                     /gene="oxlT"
                     /locus_tag="VGC82_19020"
     CDS             4573..5814
                     /gene="oxlT"
                     /locus_tag="VGC82_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEY0425417.1"
                     /translation="MTETTASIPAISTTTAATRWVQLGLGLICMMAISSPQYVWTLFT
                     KPLGSALGVAPASLQVTFSLLIVLQTFFSPFQGWLVDRFGPKLLIGVGGVLSGLSWVL
                     ASYASSLTMLYLTYGVIGGLGTGIVYVGVVGQMVGWFPDRRGFAAGVVAAGYGMGAIV
                     TTFPITNSLAAVGYQMTLLQYGAIFALVGLLAALGLKPPPTAHRADAPGVSTGTMLRS
                     PVFWLMFVMMTMMSTSGLMVTSQMAAFAGEFGITKAVVWGLAALPLALTIDRFTNGLT
                     RPFFGWVSDKIGRENTMCLAFALEGVAMALWLASRGDPLLFVLLSGVVFFGWGEIFSL
                     FPSTLTDTFGTRNATRNYGCLYMAQGIGAIFGGPMASLLHDATGTWTSVFGVAIAADL
                     LTALLAIAALKPLRARYPRAA"
     gene            complement(5818..6441)
                     /locus_tag="VGC82_19025"
     CDS             complement(5818..6441)
                     /locus_tag="VGC82_19025"
                     /inference="COORDINATES: protein motif:HMM:NF016705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY0425418.1"
                     /translation="MSGFFYYLLTFAEAATSVFGLRFAYEQPHYAVLQDLGENVEIRR
                     YEPSLAIEATVDGTDRDKAMSEAFTLLFRYITGANRQDRKIAMTAPVQTDSERIAMTT
                     PVQTSSERGTLRMRFFLPRAVAAAGAPAPLDPRLHLIEMPPMTIAALRYSGVATQVAR
                     DHEAATLFGALARSAWRPDGAVLQLSYDPPFTIPFLRRNETAVKVVR"
     gene            complement(6487..7560)
                     /locus_tag="VGC82_19030"
     CDS             complement(6487..7560)
                     /locus_tag="VGC82_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425419.1"
                     /translation="MTADPRIEVSRLTVRYNDVVAVDAIDFTIQPGELVTLLGPSGCG
                     KTTTLRAIAGLEDPTSGTIRLGGETVFSSAERRNVPTEKRGLSMVFQSYAIWPHMTVA
                     ENVAYGLNVRKMPRGEVDQNVRRVLDLVQMSRFADRPASRLSGGQQQRIAVARAIAFS
                     PTVLLFDEPLSNLDAKLRTEMRVELRELQRRLDITSVYVTHDQEEALAISDRVIVMNQ
                     GGIEQIGSPEIIYNRPESIFVADFVGAANLIRGQVRRGDDGFSVFETVGGLLLKADKP
                     GNGTETTLALRSAYVTLQREAGAGANVARGTIHRRMFHGDFIQYVVDWPAGQLIVRRP
                     PTEMIEEGAEVTIAFAPEHCILL"
     gene            complement(7557..9236)
                     /locus_tag="VGC82_19035"
     CDS             complement(7557..9236)
                     /locus_tag="VGC82_19035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY0425420.1"
                     /translation="MLPRLGVAGLSMLLVLLLCAALVIYPVVFIVAESLNTGEPGVFP
                     PGEISLRNFAGMSEDVDVLVNTLMVAFGATIMAVAIGFVLAWILTRTNIPGRGRLERL
                     MELPYYMTPLVGAMAWGILASPKTGLLNQAWRVLGGRTDVFDIYSPIGIAWVMALFEG
                     TVAFVMISAAMKSMDPSLEESSRVLGAGKMRTALRITLPLVAPGVLSAAVFVFAEMLG
                     AFAAAFVLGIPGRFYVVTTAIWEATQSFPPDYGRAAALGLSLFAAMVVTLTIAKLIMR
                     RGSHATITGKAFRPRPVDAGSARWLLLAIAWGYIVLSVILPLAALLLTSFQRFATVIL
                     SDSIFTLSNYQTALSTGTLGQAFVNSLILGLSVATIGVPIIGVLTWIIYRSKLNGRTA
                     IEYVVMFPQAVPRLIFGLGLLWAWINIPIPIYGTLWLLGIAYFTVFLPLGLRTLAGVV
                     MQIDRSLEECARVCGASWTHQMRTVSLPLLRPGIAAAWLLIFIASIRELGASVFLMGP
                     RSKVISPAIINAWLSSSSELSAAMAMILTATVFVAVIVLFAVARRITGTPT"
     gene            complement(9294..10376)
                     /locus_tag="VGC82_19040"
     CDS             complement(9294..10376)
                     /locus_tag="VGC82_19040"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0425421.1"
                     /translation="MVPVYCFRAAALALVLMFAWGGAAPALAQGTCTNPRQMDGFKTC
                     ADVEKAEQEAGLVLYSPDPEENSAKLMEAFHQAFPKITTNFIRLQTGALYQKLMTERR
                     AKVYQVDVLQLTDMGFVLDFQKRDGYARYISPEMNAYQSDFKSSPEGYWTWGAIIVAG
                     LAYNPKLVKPEDAPKSWKDALDPKYAGVINAKTSTSGLQHVTWYELKSLYGDEYFQKF
                     GDLKPRGFDSYVQQFDRMVSGQDSVVHTAQYSAYLLAKAKGAPIEFVFPEEGLTLTPG
                     VLGVVAEAPHPNAAQLFMDWFLSKQGQKVYDRISSLNSPRTDADPPPGGMTFDKVKLL
                     YPKDWQAFLASHDQFVKAWSKVTGVR"
     gene            10634..>11146
                     /locus_tag="VGC82_19045"
     CDS             10634..>11146
                     /locus_tag="VGC82_19045"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0425422.1"
                     /translation="MQQHKIVARVHHSLLADYERRLLVWLAAQLPAIVTPTRLTLLGF
                     AGALLSGLSYAATSFSTGFLWLAIMGLIVNWFGDSLDGTLARYRKTERPQYGFFIDHT
                     TDVASQVVIFLGLGISPYMHLETACLALMSYWMAALYTFIRAIATNIFQISYYGVGPT
                     EIRLALIAYNM"
BASE COUNT         2062 a         3516 c         3469 g         2099 t
ORIGIN      
        1 ccagacgggc ctttttcgaa gtggcgggcg atttgacggg acacactttg gaggggttca
       61 aattttggcg gatttctgcg ggttttagcg tgtagacagc agactcatga acttttcccc
      121 ttgattggcc gacgggccaa atgcagccct taccgagttt cgggcgactt acccactcag
      181 gtcaacttac aggccagccg tgtacaggct acccgtttat aggccagcct gggaaatccg
      241 actttatcat ggatcgatgc aagggcactt ttcgttgttc gaatcggcac cggtagaaag
      301 cgcccgagaa gccgaccgat aggcgcgcag gttggtatgg cggtggcgtg ctgaatcaac
      361 aagacatcga cggccgttga cccggcgacg agtcaatatc aaggttcgtc gggctagcca
      421 cggccagcga atttcgaagg aagacgtaca acacgaccag taacggcgaa taagccgcgg
      481 tcggatacag ggaaggcaaa caaaatggac gcattggaac agacgacaat gaggaaggtc
      541 gggcgccgcc tgatcccctt tctcatggtg tgttatttct tcaactttct ggatcgcgtg
      601 aacgtcagct ttgcggcctt ggaaatgaac aaggacctgg gcttctccgc ctccgccttt
      661 ggtctgggtg ccggcatcct gttcttcacc tacatgctgt gcgaaacgcc atcctggtca
      721 aagtcggcgc gcgtatctgg ctcgcgcgca tcatgttcac ctggggcatc atagccgccg
      781 gcatggcatt cattgcgggc gaaaagagct ttctgacgat gcgggccctg ctgggtgcag
      841 ccgaggccgg tttcttccct ggaatcatct attacctgac cctgtggttc cccggcacgt
      901 accgcgcgcg agtcacgggg cttttcatgg cctgcatccc gctatcggct gccatcggcg
      961 cacccatctc cacgtcactg ctgtacctcg acggcgcact gggatttcgc ggctggcaat
     1021 ggatgttcct gctggaaggc ctgccgtcca tcgcgcttgg cgtagccacc tactttttcc
     1081 tgacaggcac cccaaccgag gccaaatggc tcggcgccga agagcgcggc tggctggatg
     1141 cacgtatcaa ggcggagggc gcccgcaagg ccggcgcgtt gtcactgagc atgctcgaga
     1201 cgctgatgaa cgggcgggtg ctcgcgttga gcatcgtcgc cttctttatc gcctcccagt
     1261 tgctcggcgt tggcttcttc ctgccgacaa tcgtcaaggg gttcggcctg acgaacatgc
     1321 agaccggcct ggtgtccatc attccgccag cctgcgggtc agtggcgatg atctggtggg
     1381 ggcggcactc ggaccggttg atggagcggc ggtatcacct cgccagcgcg ctgatagtcg
     1441 gcgccgtcgg cataatcgcg gccggattta tcgacgaccc ggtgttgaaa atgctggcgt
     1501 tcaccatctc ggcgatcggc ttgaatgcgt cactgccggt gttctggacg ctggcgccgg
     1561 cgttcctgac cggggcttcg gcggcggtcg gcattgcctt catcaactcg gtggccgcgc
     1621 ttggcgcatt cctcgcgccc ttcatgctgg gactggtgaa agacgccacg ggaagcttca
     1681 ccggcggatt gtacctcctc ggaggcgggg tgatcatcgc cgcgctgttc gtcctggcgt
     1741 tcgctcacgc gcatgacctc gagcgggagc cgcaggttca accggcagga taagccgacc
     1801 gacgctttgg ctgtgctccg ggttcggcac cccgccagtg ccgtttacct gcgactgcgt
     1861 ctatctgcgg tcggcgctga ggcggccggc gccgaacgcc acgacctgaa gaaagccgcc
     1921 tgccatgcag acattcttca tgaagttgat catctgctcg cggctttccg gatggtagtg
     1981 ggcgaccatg gccgtggcga tgcaccacgc cgccaacacc agcccggtca gccgggcgcg
     2041 gaatcctacc aggaacagta tgccggcgcc gatctcgatc aggagggtca gcgcgtaagc
     2101 cgcgcccggc atcggcagcg ggattttcgc gaagtacccc atggtggccg ccggggccat
     2161 cgccttcatg attccgccct ggatgaagat cgcgctcatc agaaagcggc cggcaaggcc
     2221 gacccaatcc agcgagtccg ttctcatcca acgagttccg tgccggcgaa gaaataggcg
     2281 atctcggccg ctgcattctc gacgctgtcc gaaccatgca ccgagttggc ttcgatgctt
     2341 tcggcgaaca gggctcgaat cgtaccggga tcagccttct tggggtccgt ggcacccatg
     2401 aggtcgcggt tgcgggccac ggcgttctca ccttccagga cctgaacgac gacgggacca
     2461 gagatcatga aggtcaccag gtcgcggaag aacggccgag cggcgtgaac gccatagaag
     2521 gtttcggcct gcgcctgggt catgtggatg cgcttctggg caataatccg cagacctgct
     2581 gcttccagca cggcattgac cttgccggtc aggttccggc gggtcgcatc cggcttgata
     2641 atcgagaaag tgcgttcggt cgccatcgcg gcctcctgta tgaattcagg cggctgagta
     2701 aggcgttgca cacccgaggg caaggcatta gctggctctc ggcaggccat cgaggcctat
     2761 ccgtttatgc tgatatccgt cagccgccgg tgctcgtcca tcgcgtaacg gtcggtcatg
     2821 cccgccacgt aatccgcgac ggtagcggcg gagcgttgca tcccaccctc gcctgcgcgg
     2881 gcgcgccagt cgtccggcaa cagcgtcgga tcggtgtgca gcaaccgaaa caggtcctcg
     2941 gtcaggcgca tggccttggt ggacatgcgg ttcacccgcc agtggcgata catgcgggcg
     3001 aacaggaagt ccttgatcgc ctgattggca tcggccatcg ccggactgaa accgaccacg
     3061 cgctgcttat gccggcggat cgcgtccggg ctgtcggggt ccagcttggc caaccgcttt
     3121 ccggtttcgg tgatcaggtc ggtaaccata tcgttgatca cccgccggag catctcgtgg
     3181 cgcagccgtg gcggcggaac atccaggctg gacatgcggg cagcggcgag ggacgcgccg
     3241 accaagggca aatgcttcac atcatccacc gtgaagagac gagcgcgcag gccgtcatca
     3301 aggtcgtggg tatgatacgc gacgtcgtcg gcgatggccg caacctgggc ttccgcggcc
     3361 ggctgagtat gcagatcgag tttgtgatgg tcgttgtatt cggcgatgta gcgcgccggg
     3421 tggcgcaacg ggccattgtg cttgaccagg ccttccagcg tttcccaggt caggttcagg
     3481 ccgtcccagc cggcgtagcg gctttccagc agcgtcacga tacgcaagct ctgagcgttg
     3541 tgatcgaaac cgccgaatgg tttcatgacc acgttgagcg cgtcctcgcc ggcatggccg
     3601 aacggggggt gacccaaatc gtgcgccagg gccagcgcct cggtcaggtc ttcgtccagg
     3661 ccgagcgccc gggcgatcga gcgagcgatc tgggcgacct caatgctgtg ggtcagccgg
     3721 gtgcggtaga aatcgccctc atgattgacg aaaacctgtg tcttatactg gagttttcga
     3781 aatgccgagc tgtggatgat gcgatcgcgg tcgcgttgcc agggggagcg ggtttccgcc
     3841 tcgggttcat gatgcagccg gccgcgcgcc gtcgccggcg aaacagcgta cggggcgaga
     3901 ggagggcgcg tgaacatgtc aagcggatac accttgcacc gccgcgcttc aatcagggca
     3961 cccccgcgct tcaatcaggg ttcgccccac agcggcaagg tccccgcatc gcttcgctca
     4021 gggtggtttc accgcacagg gtggtttcac cgcacaggcc gcttcaattc cgtggtgaag
     4081 aagcctatct tagcgtcatg gaacagttcg ccgttaccga ccgcgccgcc gcccgtatcg
     4141 ccgagatcgt cgccgcgacg aaaccggccg ctgacaccgc cttgcgtgtc gcggttctcg
     4201 cagggggatg cagcggcttt cagtatcgct tcgagctgga cccgcaaacc cagccggacg
     4261 acctcgtcat agagcacggg cccgcccgcg tggtggttga cgccgtcagc ctggatctcc
     4321 tgagcggctc cgaactcgac tacaccgatg aattgatggg aagccacttc gcggtgcgca
     4381 acccgaatgc caaatcggcc tgcggctgcg gcacaagctt ctcgatcgac tgaaaataca
     4441 gcagtcgggc tcgcatctgg tgtggaaacg ggcaggcgtc tccgtcgcga ggtgccgaca
     4501 tgctgcgtca tagcggcata cacttggcgt aacatttgca cgggctgagc ggtcaccgaa
     4561 gggccgacca gcatgaccga gaccaccgcc tctatccccg ctatttccac caccaccgcc
     4621 gctacaagat gggtccagct cggtctgggc ttgatttgca tgatggcgat ctccagcccg
     4681 caatatgtct ggacgctgtt caccaagccg ctggggtcgg cgctgggtgt ggcaccggcg
     4741 tcgctccagg tgacgttctc gctgctgatc gtgttgcaga cgtttttctc gccgtttcag
     4801 ggatggctgg tcgatcggtt cgggccgaaa cttttgatcg gtgtcggcgg cgtgttgtcg
     4861 ggcttgtcct gggttctggc ctcctacgcc tcgtcgctga cgatgcttta tctgacctac
     4921 ggcgtgatcg gcgggcttgg caccggcatc gtctatgtcg gcgtcgtcgg gcagatggtg
     4981 ggatggttcc ccgaccgtcg aggcttcgcc gcgggtgtcg tcgccgccgg gtacggcatg
     5041 ggggcgatcg tgacgacgtt tccgatcacc aactcgctgg cggccgtggg ctatcagatg
     5101 acgctgctgc agtacggggc gatcttcgcg ctggtcgggc tgctggcggc attggggctt
     5161 aagccgccgc cgaccgcgca ccgggcggat gcgcctgggg tttccaccgg gacgatgctg
     5221 cgatcgccgg tgttctggct gatgttcgtc atgatgacga tgatgtccac ctcggggctg
     5281 atggtaacct cgcagatggc cgcgttcgcc ggcgagttcg ggataacgaa ggcggtggtg
     5341 tggggtcttg cggcgctgcc gctggcgctg accatcgacc ggttcaccaa cgggctgacg
     5401 cggccgttct tcggctgggt gtcggacaag attggccgcg agaacacgat gtgcctggcg
     5461 ttcgcgctag agggtgtggc catggcgctg tggctggcgt cgcgcggcga cccgctgctg
     5521 ttcgtgctgc tgtcgggtgt ggtgtttttc ggctggggcg agatcttctc gttgtttccg
     5581 tccacgctga cggacacgtt tggcacgagg aacgccacgc gcaactacgg ctgcttgtac
     5641 atggcgcagg ggatcggggc cattttcggc ggcccgatgg cgtccctgtt gcacgacgcg
     5701 accgggacat ggaccagcgt cttcggggtg gcgatcgcgg cggacctgct gacggcgctg
     5761 ctggcgatcg ctgcgttgaa gccgctgcgg gcgaggtatc ctcgggccgc ttgatgttca
     5821 acgaacaacc ttcaccgcgg tctcattgcg tctaaggaac ggaatcgtga acggtggatc
     5881 gtagctcaac tgtaggacgg ccccatccgg ccgccacgcc gacctcgcca gcgccccaaa
     5941 cagcgtggct gcttcatgat cgcgcgcgac ctgcgtggcg accccggaat agcgcagcgc
     6001 cgctatcgtc atcggcggca tctcaatcaa atgcagccgg ggatcgagtg gcgccggcgc
     6061 cccggccgca gccaccgccc ggggcaaaaa gaaccgcatc cggagcgttc cccgctcgct
     6121 gctcgtctgc accggcgtcg tcatggcgat ccgctcggaa tccgtctgga ccggcgcggt
     6181 catcgcgatc ttccgatcct gccggttggc accggttata tagcggaaca gcaaggtaaa
     6241 cgcctcgctc attgccttgt cgcggtcggt tccatcaacc gtcgcctcga tcgccagact
     6301 tggctcgtag cgccggatct ccacgttttc gccgagatcc tgcagcaccg cataatgcgg
     6361 ctgctcatac gcgaagcgaa gcccaaacac cgaagtggcg gcttcggcga acgtgagaag
     6421 atagtagaaa aagccggaca tcgaagccct ccccgcggcc ttgaagatgg ggtcatgccg
     6481 ggcgttctac agcaggatgc aatgctccgg tgcgaaggcg atcgttacct cggcgccttc
     6541 ctcgatcatc tccgtcggcg gccgccgcac aatgagctgc ccggccggcc aatcgaccac
     6601 gtactgaatg aaatcaccat ggaacatccg ccggtggatc gtgccacgag cgacgttcgc
     6661 tccagccccg gcttcacgct gcagcgtgac ataggccgac cgcagtgcca gcgtggtttc
     6721 cgtcccgttc cctggcttgt ccgctttcag caacaggcct ccgacggttt cgaaaacgct
     6781 gaagccgtcg tcgccccgcc gcacctggcc cctgatcagg tttgccgcgc caacgaagtc
     6841 cgcgacgaaa atgctctcag gccgattata gataatctcg ggcgagccga tctgctcgat
     6901 gcccccctgg ttcatcacga tgacccggtc ggagatcgcc agcgcctcct cctggtcatg
     6961 cgtcacgtaa accgacgtga tatcgaggcg gcgctgcagc tcccgcaact ccactcgcat
     7021 ttccgtccgt agcttggcat ccaggttcga caacggctcg tcgaacagca acaccgtcgg
     7081 ggaaaatgcg atggcccgag cgacggcgat gcgctgctgc tgtccgcccg acaggcgcga
     7141 tgccggccga tcggcgaacc ggctcatttg caccagatcg agcacgcggc gaacgttctg
     7201 gtccacctcg ccccggggca tcttgcgcac attcagcccg taggcgacgt tttcggcgac
     7261 cgtcatatgc ggccagatgg cataggactg gaacaccatc gacaggccgc gtttctcggt
     7321 cggcacgttc cgccgttcgg ccgaggaaaa caccgtttca ccgcccagcc ggatcgtacc
     7381 gctggtggga tcttccagcc cggcgatcgc tcgcaacgtc gtcgtcttgc cgcagccgga
     7441 cggacccaac agggtcacca gttccccggg ttggatggtg aagtcgatcg cgtccacggc
     7501 gacgacatcg ttgtagcgga ccgtcagacg ggaaacctcg atacgcgggt ccgctgtcat
     7561 gtcggggttc cggtgattct acgggcgacg gcgaacagca cgatcaccgc gacgaacacc
     7621 gtcgcggtca atatcatcgc catcgccgcc gacagctctg aactcgacga cagccacgca
     7681 ttgatgatgg ccggcgaaat cacctttgat cgtggtccca tcaggaacac cgaggcaccg
     7741 agttcgcgga tcgaggcgat gaagatcagc agccatgcgg cggcgatacc cggccgcaac
     7801 agcggcagcg acaccgtgcg catctgatga gtccatgatg caccgcagac ccgggcacat
     7861 tcctccaggc tgcggtcgat ctgcatgacg acgccggcca gggtgcgcaa acccagcggc
     7921 aggaacacgg taaagtaggc gatgcccagc agccacagcg tgccatagat cggaattgga
     7981 atgttgatcc acgcccacag cagacccagc ccgaagatca gccgcggcac cgcctgcggg
     8041 aacatcacga catactcgat cgcggtgcgg ccgtttagtt tcgaccgata gatgatccac
     8101 gtcagaaccc cgatgatcgg cacgccgatc gtcgcgaccg acaggccgag gatcaggctg
     8161 ttgacgaaag cctgtccaag cgtgccggtg ctcagcgcgg tctggtagtt ggacagggtg
     8221 aagatgctgt cgctcaggat cacggtggcg aaccgctgga acgaggtcag cagcagcgcg
     8281 gcaagcggca gaatcaccga caggacgatg tagccccagg cgatcgccag cagcaaccag
     8341 cgcgcggacc ctgcatccac cggccgaggc cggaacgcct tgccggtgat cgtggcgtgg
     8401 ctgccccggc gcatgatcag cttggcgatg gtcagcgtca ccaccatggc ggcgaacaac
     8461 gacaaaccca acgccgccgc cctgccgtaa tccggcggga aggactgcgt cgcctcccag
     8521 atcgccgtgg tcacgacata gaaacggcca ggaatcccca gcacgaaggc ggcggcgaaa
     8581 gcgcccagca tttccgcgaa aacgaagacc gctgcgctca ggacgcccgg agcaaccaga
     8641 ggcagcgtaa tccgcaacgc cgtccgcatc ttgccggcac ccaggacgcg ggaactttcc
     8701 tccagcgaag ggtccatcga tttcatcgcc gccgagatca tgacgaacgc aaccgtgccc
     8761 tcgaacagcg ccatgaccca ggcaatgccg ataggcgagt aaatgtcgaa cacatcggtg
     8821 cgcccgccca gtacgcgcca cgcctgattc agcagtccgg tcttcggcga cgccaggatg
     8881 ccccacgcca tcgcgcccac cagcggcgtc atataataag gcagctccat caatcgttcg
     8941 agccggcccc tgcccggaat gttggtgcgg gtcaggatcc acgcgaggac aaagccgatc
     9001 gccaccgcca tgatcgtggc gccaaacgcc accatcagcg tgttgacgag gacatccacg
     9061 tcctccgaca tgccggcgaa gttccgcagg ctgatctcgc cgggcgggaa cacaccgggt
     9121 tcgccagtgt tgagcgactc ggcgacgatg aacacgaccg ggtagatcac cagcgccgcg
     9181 cacaacagca gcacaagcag catcgacagc cccgccacgc cgaggcgcgg taacatccgg
     9241 gcaacaccga tcatcgcagc agccccggcg gatggacaaa atccagcaag gccctaacgg
     9301 acgccagtga ctttgctcca ggccttcacg aactggtcgt gcgaggccag gaaggcctgc
     9361 cagtctttcg gatacagcag cttgaccttg tcgaaggtca tgccgcccgg cggcggatcg
     9421 gcatcggtgc ggggcgagtt cagcgaggag atccggtcat aaaccttctg gccttgcttg
     9481 gacaaaaacc agtccatgaa cagctgcgcg gcattcggat gtggcgcctc ggcgaccaca
     9541 cccagcacgc ccggcgtcag cgtcagccct tcttcgggaa agacgaattc gatcggggcg
     9601 cctttcgcct ttgccagcag gtacgccgaa tattgcgcgg tgtgcacgac cgaatcctgg
     9661 ccactgacca tgcggtcgaa ttgctgcacg taggaatcga aaccacgcgg cttcaggtca
     9721 ccgaatttct ggaaatactc gtcgccgtag aggcttttca gctcatacca ggtgacatgc
     9781 tgcaaaccgg atgtggatgt cttggcgttg atcacgccgg catatttagg atccagcgca
     9841 tccttccagg atttcggcgc atcctcgggc ttcaccagct ttgggttata ggcgaggcca
     9901 gcgacgatga tcgcgcccca ggtccagtag ccttccgggc tgctcttaaa gtcggattgg
     9961 tatgcgttca tctccggcga gatgtaacgg gcataaccgt cgcgcttctg gaaatccagc
    10021 acgaagccca tatccgtcag ttgcagcaca tcgacctgat aaaccttcgc ccgccgctcg
    10081 gtcatcagct tctgatacaa ggcgccggtt tgcagccgga tgaagttcgt cgtgatcttc
    10141 gggaaggcct gatggaacgc ttccatcagc ttggcggagt tctcctccgg gtcgggagaa
    10201 tacagcacca ggccggcttc ctgttccgcc ttctcgacgt ccgcacaggt cttgaagcca
    10261 tccatctgcc ttggattggt gcaggtgccc tgggccaagg ccggcgcggc accgccccag
    10321 gcgaacatca acacaagagc cagggcagcc gccctgaaac aatagactgg aaccatgcac
    10381 gcccctcccg gcgaccgggc ctacccggat cggccggacg ctagcgctcg ccctgccccg
    10441 ccgcaaggcg tggaccagct cggacgcccg gcataagggg cgtgccgaaa cgactattgt
    10501 cagttcccgt tcgttttgct tccagcgcag caaaaagctt aagtcggagc aaaacaacgt
    10561 tacctagccg cagagtttaa ggtgtaacaa ccgttgccag cgaggtcagc ccacctgacg
    10621 caagagacgg aaaatgcagc agcataagat cgtggcgcgt gtccaccact cattgctggc
    10681 agattacgag cgccgccttt tggtttggct ggcggctcaa ttgccagcaa tcgtcacgcc
    10741 gacccgcctc acgctccttg ggttcgccgg ggcgctgctg agcgggcttt cctatgcggc
    10801 cacctcgttc tcgacgggct tcctgtggct cgcgataatg ggcctgatcg tcaactggtt
    10861 cggcgattcg ctggacggaa ccttggcgcg gtaccgcaag acggaacgcc cgcagtatgg
    10921 cttcttcata gaccacacca ccgacgtggc atctcaggtc gttatcttcc tcggcctggg
    10981 catttcaccg tatatgcacc ttgaaacagc ctgtctggct ctcatgagct actggatggc
    11041 ggcgctctat acgtttatcc gggcgatcgc cacgaacatc ttccaaatca gctactatgg
    11101 agttgggccg acggagatcc gactggcgct gatcgcctac aacatg
//
LOCUS       DASWQN010001114         3297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1558648, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001114 DASWQN010000000
VERSION     DASWQN010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558648"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VGC82_19050"
     CDS             <1..529
                     /locus_tag="VGC82_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY0425423.1"
                     /translation="LTAPLILGGIIAIRWEGPGPVLYRQERVTADGKTFFIMKLRSMR
                     VNAEAGGAVWAREKDNRITRVGNFLRRTRIDELPQLVNILRGEMSFVGPRPERPMFVK
                     ELAEKIPLYNERHMVKAGLTGWAQINYPYGASIDDARSKLSYDLYYVKNFSILFDFVI
                     LLRTLRVVLWPSGAR"
     gene            complement(477..1727)
                     /locus_tag="VGC82_19055"
     CDS             complement(477..1727)
                     /locus_tag="VGC82_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425424.1"
                     /translation="MLHSLSRHEIDSPYAWFRLAVSVVLSAIGGVGMWSVVVALPAVQ
                     ADFGAERGAASLPFTLTMIGFGVGGVIMGKMADRRGIILPIMIGAGSLAAGYVLASLS
                     GGLGTFSAAYLLIGTFGTAATFVPLMADISFWFEKRRGIAVAVCACGNYLSGAVWPTL
                     IEAAIRAHGWRTAHLAIGVFCLVIMPPLAMLLRSRPPVRAAGAPLPASSLRRVNLPPR
                     VLQTLLSIAGLACCVAMSMPQVHIVAYCGDLGYGAARGAQMLSLMLSFGIVSRIASGF
                     LADRFGGVATLLIGSFAQMTALSLYLTMDGLTSLYVISALFGLFQGGLVPSYAIIVRE
                     SFPAKEAGARVGVILMMTLLGMALGGWMSGVIFDFTGSYRAAFANGVAWNGLNFIIAA
                     FLLFRSSLTARRSATAPHAESAAE"
     gene            complement(1999..>3297)
                     /locus_tag="VGC82_19060"
     CDS             complement(1999..>3297)
                     /locus_tag="VGC82_19060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HEY0425425.1"
                     /translation="PVALCLMCHVTRGDYMRIEIICTGDEVLTGKITNTNFSYMAQKL
                     EDVGLSVHWGTTVGDDRDSLLAAFQLAGTRADAVIVNGGLGPTVDDLSQEIAAQAAGV
                     ELVLHEEWLAKMESFFTRRMRTMPPNNVKQAMLPATAEMIDNPVGTACGFALDIGRAR
                     FFFTPGVPRELRRMLEEQVIPRILARAGAPASIYLKRFHSYGLGESHVDQLLAGVEAL
                     DPAGGVKLGFRAHYPQLETKLTVRGTDMDDVHRKLAPVTAEVRKRLGNFIMAEDDQTL
                     EGVTLAALAAENATLSVVETFTAGQIAARIAHLPGAEKIFRHGTVVRHAPSLFGVHLP
                     PELDQDTTEAVARAAREQTGASHALAVLIELDEGADRIDLGGTIWIGIATAGGTEFRR
                     SRILGGREWVRLGAVELALDCLRRFLQGLPVVERIDFEKT"
BASE COUNT          628 a         1051 c         1087 g          531 t
ORIGIN      
        1 gctgaccgcc ccgctgatcc tgggcgggat catcgccata aggtgggaag ggccaggccc
       61 ggtgctttat cggcaggagc gggtgaccgc ggacggcaaa acctttttca tcatgaagct
      121 gcgcagcatg cgggtgaacg ccgaagccgg cggcgcggtc tgggcgcggg aaaaggacaa
      181 ccgcatcacg cgggtcggca atttcctccg gcgtacccgc atcgacgaat tgccgcagtt
      241 ggtgaacatc cttcgcggcg agatgtcgtt cgtcggcccg cgtccggaac gcccaatgtt
      301 cgtcaaggaa ctggcggaga aaatcccgct gtacaacgaa cggcacatgg tcaaggcggg
      361 cctgaccggc tgggcgcaga tcaactaccc ctatggcgcc tcgatcgacg atgctcggtc
      421 caagctcagc tacgacctat actatgtaaa gaatttttca attctgttcg acttcgtcat
      481 tctgctgcgg actctgcgtg tggtgctgtg gccgagcggc gcgcggtaag cgaggagcgg
      541 aacagcagga aggcggcgat aatgaagttc agcccgttcc acgccacccc attggcgaag
      601 gcggcgcgat aggagccggt gaagtcgaag atgacgcccg acatccagcc gcccagcgcc
      661 atacccagca gggtcatcat cagaatgacg ccgacccgcg cgccagcctc cttggccgga
      721 aagctttccc gcacgatgat cgcatagctg ggtaccaggc cgccttggaa caggccgaac
      781 agcgccgaga tcacatacag cgacgtcagc ccgtccatcg tcaggtacag cgacaacgcc
      841 gtcatctgag cgaacgagcc gatcagcagt gtcgcaaccc cgccgaaccg gtcggcgaga
      901 aagcccgatg cgatgcggct gacgatgccg aagctcagca tcagcgacag catttgcgcg
      961 ccccgggcag cgccgtagcc caggtcgcca cagtaggcga cgatgtgcac ctggggcatc
     1021 gacatcgcca cgcagcaggc aagaccggca atcgacagca aggtttgcag cacacgcggg
     1081 ggcaggttga cgcggcggag cgaggacgcg ggaagcggcg cgccggcggc tcggactggc
     1141 ggacggctgc gcaacagcat ggccagcggc ggcatgatca ccaggcagaa aacgccgatc
     1201 gccaggtgag cggtgcgcca gccatgagcg cggatggcgg cctcgatcag ggtcggccag
     1261 accgcgccgg acaggtagtt gccgcaggcg cagaccgcca cggcgatgcc gcgccgcttt
     1321 tcgaaccaga acgagatatc cgccatcagc ggcacgaacg tcgccgcggt gccgaaggtg
     1381 ccgatcaaca ggtaggcggc cgaaaacgtc cccaagccgc cggaaagcga ggccaggaca
     1441 tagcccgcgg cgagcgagcc cgccccgatc atgatcggca ggataatgcc gcgccggtcc
     1501 gccatcttgc ccatgatcac gccacccacg ccaaacccga tcatggtcag cgtgaagggc
     1561 aggctggccg cgccgcgttc ggcgccgaaa tcggcttgaa ccgcgggcag ggcgacaacg
     1621 accgaccaca ttccgacgcc gccgatcgcc gacagcacga ccgatacggc cagccggaac
     1681 caggcatagg gcgagtcgat ctcatgccga ctcagtgagt gcagcacttt tgtcctcccg
     1741 ttgggcttgg cctgatgcgc cttgcgcctt ggtttcgccc gagcggttac cgcccggaca
     1801 tgctcgttac ttggttacag gcgggatggg ttacccgcgt gcccgcgttt caacggatgc
     1861 ggttgacgcc atgaccggac caccaggggt tatgagttat caccttgggg tcgggcgcac
     1921 gctggcagat cggatcctgc atggaacgca gcgtatcgaa cacagcgcat ggaaggcggc
     1981 cgttatcata atcatgccct aagtcttctc gaagtcgatg cgctcgacca ccggcaagcc
     2041 ttgcaggaag cggcggaggc agtccagggc caattcgacc gcacccaatc ggacccattc
     2101 gcgtccaccg aggatacgcg aacgccggaa ttcagtcccg ccggcggtgg cgatgccgat
     2161 ccagatggtg ccgccgaggt cgatccggtc ggcgccctca tccagttcga tcagaaccgc
     2221 cagggcgtgg ctcgcgccgg tctgctcgcg cgctgcccgg gcaacggcct cggtggtgtc
     2281 ctggtcgagt tccgggggaa ggtggacgcc gaacagggat ggtgcgtgac ggacgacggt
     2341 gccgtgacgg aagatttttt ccgcgcccgg caggtgggcg atacgggcgg cgatctggcc
     2401 ggcggtgaag gtttccacca ccgacagggt ggcgttttcg gctgccagag ccgccagcgt
     2461 cacgccttcc agggtttggt cgtcctcggc cataatgaag ttgcccaggc gcttcctgac
     2521 ttcggcggtg accggcgcga gcttgcggtg gacgtcgtcc atgtcggtgc cgcgcaccgt
     2581 cagcttggtt tccaactgcg gataatgtgc gcggaaaccc agcttgacgc cgcctgccgg
     2641 gtccagcgct tcgacccctg ccagcaactg gtccacatgc gattccccga gaccgtacga
     2701 atgaaagcgc ttcaggtaga tcgatgccgg cgccccggcg cgggcgagga tgcgagggat
     2761 cacctgctcc tccagcatac ggcgcagctc gcgagggacg cctggggtga agaagaagcg
     2821 cgctcggccg atatccaggg cgaaaccgca ggcggtgccg accggattgt cgatcatctc
     2881 ggctgtcgcg ggcagcattg cctgcttgac gttgttcggc ggcatggtgc gcatgcggcg
     2941 ggtgaagaag ctttccatct tcgccagcca ttcttcatgc aggaccagtt ccactcccgc
     3001 ggcctgggcg gcgatttcct gcgacaggtc atcgaccgtt ggcccgaggc cgccattgac
     3061 gatcaccgcg tcggcccggg tgccggcgag ctggaacgcg gccagcaggg agtcgcggtc
     3121 gtcgcctacg gtggtgcccc aatgcaccga cagcccgaca tcctccagct tttgcgccat
     3181 gtaggagaag ttggtgttgg tgatcttgcc ggtcagcacc tcatcgccgg tgcagataat
     3241 ttcgatgcgc atgtagtccc ccctggtaac gtggcacatt aggcaaagcg ccaccgg
//
LOCUS       DASWQN010001115         4099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_503696, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001115 DASWQN010000000
VERSION     DASWQN010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503696"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1007)
                     /gene="lgt"
                     /locus_tag="VGC82_19065"
     CDS             complement(90..1007)
                     /gene="lgt"
                     /locus_tag="VGC82_19065"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY0425426.1"
                     /translation="MMTVLLFPQFDPVLVQLGPLSIRWYALAYIASLVIGWRLVRRFV
                     QLHPAVATPLQVDDFLTWATLGVVFGGRLGYVLFYQPGVYLAHPGMILAVWEGGMSFH
                     GGMLGVGIAIIVFCRRNAIPILGFADRIAIITPIGLGLGRVANFINGELWGRPAPLWW
                     PGAMVFPRATPTAQPSLEAYEMLRQTACAQLLPMQCVPRYPSQLYEAAMEGVLLFLLM
                     LVVSRRESLRVRFGLLTGIFLCGYATARIIGEHFREPDAFLGFLPFGTTMGQLLSIPM
                     FLAGLWLIVRARPVDRQTADPVSVTADRI"
     gene            complement(1004..1984)
                     /gene="rfaD"
                     /locus_tag="VGC82_19070"
     CDS             complement(1004..1984)
                     /gene="rfaD"
                     /locus_tag="VGC82_19070"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019087898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEY0425427.1"
                     /translation="MILITGGAGFIGSNLQAVLARRGHETVVADTLGSDGKWHNLAKH
                     PPTRIVPPDDLDRFLTSHPPVEVVFHLGAISETTAIDGDHTWATNVELSRRLWEWCAT
                     RGVRMIYASSAATYGDGAQGFDDDFSLPALERLRPLNLYGWTKHAFDLLVAKTLLKRR
                     EHPPQWAGLKFFNVYGPNEYHKGRMISVVKVKHDEVVAGAPARLFKSDQTGLADGAQS
                     RDFIWVGDVVDVMLWLMETPAVSGLFNLGTGRARTYLDLAHAVSDAAGQKRAVEFIDM
                     PASLQGQYQSFTEAPMERLRAAGYAGQFTPLEEGIRQYVQNHLAKTDRFV"
     gene            2051..2368
                     /locus_tag="VGC82_19075"
     CDS             2051..2368
                     /locus_tag="VGC82_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEY0425428.1"
                     /translation="MADRPKFFDDLAGVAGGAISALAGLREEAESMMRTRMDDLIQRL
                     DLVRREEMAAVQELAANARAGQEAAEAKVGTLEGRLAALEARVAALELPVTDSGGDAP
                     AIL"
     gene            2584..3084
                     /locus_tag="VGC82_19080"
     CDS             2584..3084
                     /locus_tag="VGC82_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEY0425429.1"
                     /translation="MSASLTTSDNAANPLDMMEQIVAANEWEFDRRSDSEMAAEAPGQ
                     WCDYGLFFNWSHEISVMHFTCAFDLKVPEKQRAALYELLALANEKLWLGHFGLEGEEG
                     MPVFRHAVLLRGTTGASAESLEDLVDIALTECERFFPAFQFVLWAGKSPAEALAAAML
                     DCVGEA"
     gene            3242..4060
                     /gene="proC"
                     /locus_tag="VGC82_19085"
     CDS             3242..4060
                     /gene="proC"
                     /locus_tag="VGC82_19085"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY0425430.1"
                     /translation="MTSTLAAIPPILLVGCGRMGSAMLSGWREQGLAPSVAVDPAASA
                     AEFAGPDMTVVADAAAIPDGFIPAAVVLAVKPQNAAATLPAYSRFAPGAVFLSIMAGR
                     TIAGIGALLGPAAAIVRAMPNTPAAVRQGVAVGCSNPSVTPAQKALCDRLLQAIGKVA
                     WVDDEALLDPVTAVSGSGPAYVFLLAELMEQAAIEQGIPPELARLLARQTVSGSGALL
                     AASTEDAAALRKAVTSPGGTTAEALSVLMNPDAWPIAMSQAIAAATRRSRELAG"
BASE COUNT          751 a         1264 c         1372 g          712 t
ORIGIN      
        1 gagccatgaa gtggtccagg cgttcagggt gttcaggcat ggccgatccc gggacaggaa
       61 atgtgcgatg ccagcatgac gatgatccgc tagattctgt ccgcggtcac gcttactgga
      121 tctgccgtct gccgatcgac cggccgtgcc cgcacgatca gccacaggcc ggcaaggaac
      181 atcgggatcg acagcaattg ccccatggtg gtgccgaacg gcaggaaccc cagaaaggca
      241 tcgggttcgc ggaagtgttc gccgatgatc cgggccgtgg cgtagccgca caggaaaatg
      301 ccggtcagca ggccgaaccg gacgcgcagg ctttcccgcc gcgacaccac aagcatcagc
      361 aggaacaaca gcacgccttc catcgccgcc tcgtacagct ggctgggata acgcggcacg
      421 cattgcatcg gaagcaactg ggcgcaggcg gtctgccgca gcatctcata ggcttcgagc
      481 gaaggctgcg ccgtcggggt ggcgcgggga aacaccatcg cgcccggcca ccaaagcggc
      541 gccggacgac cccaaagctc tccattgatg aagttcgcga cacggccgag gccgaggccg
      601 atcggcgtga tgatggctat ccggtcggcg aatcccagga tcgggatggc gttgcggcgg
      661 cagaagacaa tgatggcgat gccgacgccc agcatgccgc catgaaagct catgccgccc
      721 tcccagacgg cgaggatcat gccgggatgc gccagataga cacccggctg atagaacaaa
      781 acgtaaccaa gccgcccgcc gaagacgacg ccgagggttg cccaggtgag aaaatcgtcc
      841 acctgcaacg gtgtcgcaac cgcgggatgc aactgcacga agcgccggac caggcgccag
      901 ccaatgacca ggctggcgat gtaggccagc gcgtaccagc gaatgctgag tgggccgagc
      961 tggaccagga ccgggtcgaa ctgcgggaac agcaggaccg tcatcatacg aagcggtctg
     1021 tcttggccag gtggttctgc acgtattgcc ggatgccttc ctccagcggc gtgaactggc
     1081 cggcataacc ggccgcgcgc agccgctcca tcggcgcctc ggtgaaggac tgatactggc
     1141 cttgcaggct tgccggcatg tcgatgaact ccacggcgcg cttctgtccc gctgcatcgg
     1201 acaccgcatg ggccagatcc aggtaggtcc gagcccgccc ggtgccaagg ttgaacaacc
     1261 cactgaccgc cggggtttcc atcagccaga gcatcacgtc caccacgtca ccgacccaga
     1321 tgaagtcgcg cgactgggcg ccatcggcca agccggtctg gtcggacttg aacagcctgg
     1381 cgggggcgcc ggcgacgact tcgtcgtgct tgaccttcac gacggagatc atgcggccct
     1441 tgtgatactc gtttggacca taaacgttga agaacttcag cccggcccat tgcggcgggt
     1501 gttcacggcg tttcagcagg gtcttggcga ccagcagatc gaaggcgtgt ttggtccagc
     1561 catacaggtt cagcggccgc aaccgctcca gcgccggcag cgagaaatcg tcgtcgaagc
     1621 cctgggcgcc gtcgccgtag gtggcggcgg acgaggcgta gatcatgcgt acgcctcggg
     1681 ttgcgcacca ttcccacagg cggcggctga gttccacgtt ggtggcccag gtatggtcgc
     1741 cgtcgatcgc cgtcgtttcg ctgatcgcgc cgaggtgaaa gaccacctcg accggcgggt
     1801 ggctggtcag gaatctgtcc agatcgtccg gtggaacgat ccgggttggc ggatgcttgg
     1861 caaggttgtg ccatttgccg tcgctaccca gcgtatcggc aaccacggtc tcgtgcccgc
     1921 ggcgcgcgag caccgcttgt aaattagagc ctatgaatcc ggcaccgcca gttatcagga
     1981 tcatggccac gctatatacg aggcctgggc ggtggtgaaa gccgcctgat cacgcaggag
     2041 cgatttagcc atggccgatc ggcccaaatt cttcgacgac cttgccggtg tggccggtgg
     2101 agcgatttcc gccctggccg gcctgcgaga ggaggccgag tcgatgatgc gcacccggat
     2161 ggacgacctg atccaacggc tcgatctggt gcggcgggag gaaatggccg cggtccagga
     2221 actggcggcc aatgctcggg ctggccagga agcagccgag gcgaaggttg gtacgctgga
     2281 aggacggctg gccgcgcttg aggctcgtgt tgccgcgttg gaactccctg tcaccgattc
     2341 cggcggcgac gcgccggcaa ttttataact ttaccgatat agttcgtctt acgccgcaaa
     2401 ccgtgaatga aacatccccg ggattttcac caaaatgatg tcttggtact tgcggcgggt
     2461 gcgaacacgt ggataggctc gatccagtgg ccaactccgg gcgactcccc gaaaaccatt
     2521 tcgggccagc actgtccggg ggtgaaggcc acgctcggcc ttccttttga cgggagaccc
     2581 cgcatgtcag catcgctgac cacgtcagac aacgcagcca atccccttga catgatggag
     2641 caaattgtcg ccgcgaacga atgggaattc gaccgccgta gtgactcgga aatggcggcc
     2701 gaggcaccgg gtcaatggtg cgactacgga ttgtttttca actggtcgca cgaaatcagc
     2761 gtcatgcact tcacctgcgc gttcgacctg aaggtgccgg agaagcagcg tgccgcgctg
     2821 tatgaattgc tggcgttggc caacgagaag ctctggctgg ggcattttgg gctggaaggc
     2881 gaagagggca tgccggtgtt ccgtcacgcg gtcctgctgc gcgggacgac aggtgcgtcg
     2941 gcggaaagcc tcgaagatct ggtcgatatc gcgctgaccg agtgcgagcg gtttttcccc
     3001 gcgttccagt tcgtgctgtg ggccggtaaa tcgcccgccg aggccttggc cgcggcgatg
     3061 ctggattgcg ttggggaggc ttaaaccagg cagcatggaa ggctacgtct ggcgccgcgc
     3121 ctgacttagc cttcctgact tggccttgtc gcgttcgacc cttgacgggt ggggtggccg
     3181 aggcaaaccg ggctgtggca tcctgtcgga gttggaggaa acgaagcacg gtgatgcgaa
     3241 catgacatcg acgctggctg ccatcccgcc gatattactg gtgggatgtg gacggatggg
     3301 cagcgcgatg ctgtcgggct ggcgcgagca gggattggca ccatccgtgg cggtagatcc
     3361 cgccgcgtcc gctgccgagt tcgccgggcc ggacatgacc gtggttgccg atgccgccgc
     3421 cataccggat gggttcatcc cggcggccgt ggtgctggcg gtcaagccgc aaaacgccgc
     3481 cgcgacattg ccggcctatt cccgcttcgc cccgggtgcg gttttcctgt ccatcatggc
     3541 tggacgcacc atcgcgggta tcggcgcgct gcttggtccg gctgcggcga tcgtgcgggc
     3601 gatgccgaac acgcccgccg cggtgcgcca gggcgtcgcc gtgggttgct cgaatcccag
     3661 cgttaccccg gcccagaaag cgctttgcga ccggctgctg caggcgatcg gcaaggtcgc
     3721 ctgggttgac gacgaggcac ttctggaccc tgtcacggcg gtctcgggca gcgggccagc
     3781 ttacgtcttc ctgctggccg aactgatgga gcaggccgcc atcgagcaag gcattccccc
     3841 cgagctcgct cggctgctgg cgcggcaaac ggtgtccggc tccggtgcgc tgctggcggc
     3901 gagcaccgag gatgccgcgg cactgcgcaa agcggtgacc agccccggcg gcaccaccgc
     3961 cgaagcgctg tcggtgctga tgaacccgga cgcctggccg atcgcgatgt cccaagcgat
     4021 tgccgcggcg acgcgccgat cccgcgaact ggcgggttga gtcgaatgcc gttgctgtca
     4081 aattcccgac atttcgtga
//
LOCUS       DASWQN010001116         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_503745, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001116 DASWQN010000000
VERSION     DASWQN010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503745"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VGC82_19090"
     CDS             complement(<1..1019)
                     /locus_tag="VGC82_19090"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY0425431.1"
                     /translation="MRLSRFFLPILKENPKEAEIVSHRLMLRAGMIRQEAAGIYAWLP
                     LGLRVLKKIEQIVREEQNRAGALEVLMPTLQLADLWRESGRYDAYGPEMLRITDRHKR
                     ELLYGPTNEEMITGIFRSYVKSYKSLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDA
                     YSFDLDEAAARRSYNRMFVAYLRTFARMGLKAIPMRAETGPIGGDLSHEFIVLAETGE
                     SAVYCDSDVLNLPVPGEEIDYEADLTPVIKQWTSVYAATEDVHDAVRFESEVAAEKRV
                     HTKGIEVGQIFYFGTKYSDTMKAMVAGADGAEVAVHGGSYGVGVSRLVGAIIEACHDD
                     AGIKWP"
     gene            complement(1258..1812)
                     /locus_tag="VGC82_19095"
     CDS             complement(1258..1812)
                     /locus_tag="VGC82_19095"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY0425432.1"
                     /translation="MSIKTPALMSTARAIIGVMALSYLFAAPARAEEVKAGDLVISQA
                     WSRATPGGAKIGGGYLTIENKGSAPDRLTGGSADVAGRVELHEMGTTDGVMKMRPLDK
                     GLTIEPGKTVKLAPGGYHLMLVDLKTPLKQGEQIPVTLEFEKAGKAKVTLDVQGIGAQ
                     APGGGGAGGHMDMQKMREHSGTKP"
     gene            complement(1878..2837)
                     /gene="copD"
                     /locus_tag="VGC82_19100"
     CDS             complement(1878..2837)
                     /gene="copD"
                     /locus_tag="VGC82_19100"
                     /inference="COORDINATES: protein motif:HMM:NF033808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HEY0425433.1"
                     /translation="MDWFGAGGNGPLIVIRAIHFAATAVTTGTLVFRTAVARPAFRSA
                     PRHFERVRAQTRHLTWSALVITVASGVIWLLLETAAMSGLPFNEAMAPSVLSTALNET
                     QFGLVSEIRLGLVIVLAVCLAYDRFPPADWLAVAAATGLTAAVAWTGHGGSTSGELGK
                     LHLAADALHLIAAASWIGGLIPLVLLLAQARKHPTVEWASIAGDAAERFSILGVVSVA
                     TLMATGFVNAWILVGSFDALFVTEYGRLLMLKIIVFAVLLVLAAINRFWLTPRLAAPP
                     ENGARIQALRQLTRNSIVEIAFGLAIFAIVGMLGTLHPAIHMM"
     gene            complement(2853..3176)
                     /locus_tag="VGC82_19105"
     CDS             complement(2853..3176)
                     /locus_tag="VGC82_19105"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEY0425434.1"
                     /translation="MLLLLVTVEATAHAMLDHAEPRVGNTVATPPREVTLWFTQKLEP
                     AFSSVTVTNAAGQRVDTGKARVSGSQMTVSLRPAGSGTFRVNWQVLSVDAHRTNGSFT
                     FQVGQ"
     gene            complement(3212..>3691)
                     /locus_tag="VGC82_19110"
     CDS             complement(3212..>3691)
                     /locus_tag="VGC82_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425435.1"
                     /translation="AITGQVGYSIPGRSSTTTFGIDPDTGDPTADTEFHPRVLNWGGT
                     IQYSMPYLKSAVVDLGLPDFINRLIPLVEANFQTPVGNTLTSGTRTTGTINPGVIWVG
                     NKFQFGIEALIPINRQSGTNVGVIAQLHFYLDDIDPRGIGKPIFGNAVQPASPFPRN"
BASE COUNT          678 a         1188 c         1151 g          674 t
ORIGIN      
        1 ggccatttga tcccggcgtc gtcatggcag gcctcgatga tcgcgccgac cagccgcgac
       61 acgccgacgc cataggagcc gccatgcacg gcgacctcgg cgccatcggc acccgcgacc
      121 atcgccttca tggtgtcgga atatttggtg ccgaagtaga agatctggcc gacctcgatg
      181 cccttggtat gcaccctctt ctcggccgcc acttcactct cgaagcgcac ggcatcgtgg
      241 acgtcctcgg tcgcggcata gaccgaggtc cactgcttga tcacgggggt gagatcggct
      301 tcgtagtcga tctcctcgcc cggcaccggc aggttgagca cgtcgctgtc gcaatacacc
      361 gcggactcgc cggtttcggc gagcacgatg aattcgtggc tgagatcgcc gccgatcgga
      421 ccggtttcgg cgcgcattgg gatcgccttc aatcccatcc gcgcgaaggt gcgcagatag
      481 gcgacgaaca tcctgttgta cgaacgccgc gccgccgcct cgtcgagatc gaacgaatag
      541 gcgtccttca tcaggaactc gcgcccgcgc atcacgccga agcggggacg ctgctcgtcg
      601 cggaatttcc actggatgtg atacagattg agcggcaggc ttttgtagga tttgacgtac
      661 gagcggaaaa tcccggtgat catttcctcg ttggtcggcc cataaagcag ttcgcgcttg
      721 tgccggtcgg tgatgcgcag catttccggg ccgtaggcgt cgtagcggcc gctttcgcgc
      781 cagaggtccg cgagctgcaa ggtcggcatc agcacttcca gcgcgccggc gcggttttgt
      841 tcctcgcgga cgatctgctc gatctttttt agcacccgca ggccgagcgg cagccaggca
      901 taaatgcccg ccgcctcctg ccggatcatg cccgcgcgca gcatcaggcg atgcgacacg
      961 atctccgcct ctttcggatt ctctttcagg atgggcagaa agaaccgcga caatcgcatg
     1021 ggcatactca aagaatcggt cgggaacggg aagccgacag tagaaaccgg atcgggtgcc
     1081 aaaacacaag ggcgcgtccg ggaaagccgg taagacgcgg attttccatt gctttgcggg
     1141 cgaccttcaa ggcgcgaata aggcgctaac ggcgcccgaa gtgccgatgc aggagagcac
     1201 aggccgtttc ccggcgccgc aggacgccga actcgcccgc cagcgtcgcc cgtccgttca
     1261 gggcttcgtg ccggaatgct cgcgcatctt ttgcatgtcc atgtggccgc ccgcaccacc
     1321 cccgccaggc gcctgcgcgc cgataccctg aacgtcgagc gtcactttgg ccttgccggc
     1381 tttttcaaac tcgagcgtga ccgggatctg ttcgccctgc ttcagcggag ttttgagatc
     1441 gaccagcatc agatggtagc cgcccggcgc gagcttgacg gtcttgccag gctcgatcgt
     1501 caggcccttg tcgagcgggc gcatcttcat cacgccatcg gtcgtgccca tttcgtgcaa
     1561 ctcgacacgg ccggcgacgt cagccgaccc gccggttagc cggtccggcg cggagccctt
     1621 gttctcgatg gtgaggtacc cgccgccgat tttcgcgccg cccggcgtgg cgcggctcca
     1681 ggcctggctg atcacgaggt cacccgcctt gacttcctcg gcgcgcgcgg gcgccgcgaa
     1741 cagatacgaa agcgccatga cgccgatgat ggcgcgcgcg gtcgacatca acgcgggtgt
     1801 tttgatactc attttttgtc ctttctggtg cccgcaggcg ggctcgaaac tctcgcggct
     1861 taacggcatc ccgcgatcta catcatgtga attgcgggat gcagcgtacc gagcatcccg
     1921 acgatggcga agatggcgag cccgaaggcg atctcgacga tgctgttgcg ggtcagctga
     1981 cgaagcgcct ggatccgtgc gccattttcc ggtggagcgg ccagccgggg cgtcagccag
     2041 aaccggttga tcgcagcaag caccagcagg accgcgaaga cgatgatctt gagcatcagc
     2101 agccggccat attcggtaac gaacagcgcg tcaaacgatc cgaccagaat ccaggcgttg
     2161 acgaaaccag tagccatcag agtcgccacg ctgacgacgc cgaggatcga aaaccgttca
     2221 gccgcgtcgc cggcgatgga cgcccattcc actgtcggat gctttcgggc ctgcgccagc
     2281 agaagcacca gcggtatcag accgccaatc cagctcgccg cggcgatcag atgcagcgcg
     2341 tcggccgcca gatgcaactt gcccaattcg ccagacgttg acccgccatg gccggtccac
     2401 gcgacggctg cggtcaggcc tgtggccgcc gccaccgcga gccaatcggc gggcggaaac
     2461 cggtcataag ccaggcatac ggccaggacg atcacgagtc ccaggcgaat ttccgatacc
     2521 agcccgaact gcgtctcgtt caatgccgtc gacaacacgc tcggagccat cgcctcattg
     2581 aacggtagcc cgctcatggc cgccgtttcc agcaacagcc agatcacccc ggatgccacg
     2641 gtgatcacca gtgcgctcca ggtcaaatga cgggtttggg cgcgcactct ttcgaagtgg
     2701 cgtggcgcgg aacggaacgc gggccgcgcg accgctgtcc tgaaaaccag ggtacccgtc
     2761 gtgaccgcag ttgccgcgaa gtgaatcgcg cgaatgacga tcaggggccc gttaccgccc
     2821 gcaccgaacc agtccatctt tggtccttac gcctactggc cgacctggaa ggtgaaactg
     2881 ccgttggttc tgtgggcgtc caccgacagc acctgccaat tcacgcggaa ggttccggat
     2941 cccgccgggc gcagcgaaac cgtcatctgg ctgccgctga cgcgtgcctt gccggtatcg
     3001 acgcgctgac cggccgcgtt ggtcacggtg acggagctga aggccggctc cagcttttgt
     3061 gtaaaccaca gcgtcacctc ccgcggcggc gtcgccacgg tgttgccgac gcggggttcg
     3121 gcgtggtcga gcatggcgtg cgcggtggct tcgaccgtca ccagcaacag cagcaacggg
     3181 atcattccgg caatagacag acggcgcatg gtcaattcct tggaaagggg ctcgcgggct
     3241 gcacggcgtt gccgaagatc ggcttgccta tcccgcgtgg atcgatgtca tcgaggtaga
     3301 agtgcagttg ggcgatcacg ccgacattgg ttccgctctg ccggttgatc ggaatcaacg
     3361 cttcgatgcc gaactggaat ttgtttccaa cccagatgac gccgggattg atggtgccgg
     3421 tcgtccgcgt acccgacgtg agcgtgttcc cgaccggcgt ctggaagttc gcctcgacca
     3481 gtgggatcag acgattgatg aaatcaggca ggccgaggtc gaccaccgcg gacttcaaat
     3541 agggcatgct gtattggatc gtgccgcccc agttcaacac ccgcgggtgg aattccgtgt
     3601 cggcggtcgg atcgcctgtg tcgggatcga tgccgaacgt cgtggtcgaa ctccggccag
     3661 ggatcgaata acccacctgc ccggtgatcg c
//
LOCUS       DASWQN010001117         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_636314, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001117 DASWQN010000000
VERSION     DASWQN010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636314"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VGC82_19115"
     CDS             complement(<1..1093)
                     /locus_tag="VGC82_19115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY0425436.1"
                     /translation="MAGTTRDPDQGQVLHRGVITAAAMLAMLMQTLDSTIANVALPYM
                     QGSMAASADEITWVLTSYVIAAAIMTAPVGWMARRFGRKKLIIGSLAGFTLASMLCGA
                     AQSLEQLVAFRLVQGMCGAAIAPLSQATMLDIYPFSRRAQAMALFSIGVTMGPIMGPT
                     LGGWLTDAYSWRFVFYVNLPFGILAILVLAIFLPETQPQTGQRFSWYGFAVLALGCGS
                     LQLLLDRGQELDWFSSREILIEAVIAALGFYLFIVHMFMADKPFLSTGLFRDRNFVAG
                     LVMVFCVSSVMLATAALLAPYLQNLAGYPVYTAGWAMAPRGLGTILSMFLASRLGMRV
                     DQRKIMACGLLILGAALYEMSTWTPDVTQG"
     gene            1489..1869
                     /locus_tag="VGC82_19120"
     CDS             1489..1869
                     /locus_tag="VGC82_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425437.1"
                     /translation="MARRFSTRILAIAASGLLASHPVRVSAAPPAGADSDLAPWYRSL
                     RQPQTNLSCCDRSDCRTVRYRIVDGHFQAFIGSEFARWQNPPYAWVDVPENSVLHRHD
                     NPTGEGVACWAGAQIICFIEGTGA"
BASE COUNT          450 a          700 c          732 g          407 t
ORIGIN      
        1 gcccctgcgt cacatcgggg gtccaggtgc tcatttcgta tagcgccgcg ccgaggatga
       61 gcagaccaca ggccatgatc ttacgctgat ccacccgcat gcccaggcgg ctggcgagga
      121 acatcgacag gatcgtgccc aggccgcgcg gcgccattgc ccagccggcg gtataaaccg
      181 ggtaacccgc caggttctgc agatacggcg ccagcagtgc cgccgtcgcc agcatgacgg
      241 acgatacgca gaataccatc accaggccgg cgacgaagtt ccggtccctg aacaggccgg
      301 tggacaagaa cggcttgtcc gccatgaaca tatgcacaat gaaaaggtaa aaaccgagtg
      361 ccgcgatcac cgcctcgatc aggatttcgc gcgacgagaa ccagtccagt tcctgtcccc
      421 ggtccaaaag gagttgcagc gagccgcaac ccaaggcgag cacggcgaac ccgtaccaac
      481 tgaagcgttg gccggtctgc ggttgggttt ccggcaggaa aatcgccaga acaaggatcg
      541 ccaggatccc gaacggcagg ttcacgtaga atacaaaccg ccagctataa gcgtcggtca
      601 gccatccgcc cagggtcggt cccatgatcg gacccatcgt gacgccgatg gaaaacagcg
      661 ccatcgcctg tgcccggcgg gagaacggat agatgtccag catcgtggcc tgggacagtg
      721 gggcgatggc ggcaccgcac atgccttgca ccagacggaa ggcgaccaac tgttcgagcg
      781 attgcgccgc gccgcacagc atagacgcca gggtgaagcc ggccaacgag ccgatgatca
      841 gcttcttgcg tccgaagcgg cgcgccatcc agcccacggg cgcggtcatg atcgccgcgg
      901 cgatgacgta tgacgtcagg acccaggtga tctcatcggc cgatgcggcc atgctcccct
      961 gcatatacgg cagcgcgaca ttggcgatgg tggaatccag ggtctgcatc agcatcgcca
     1021 gcattgcggc cgcggtgatc acgcctcggt gcagaacctg gccctggtcc ggatcgcgcg
     1081 tcgtgccggc cattgggcta tcgccgcccg gttcggcggt tcggcggctg agagttgaac
     1141 agcatggtcc cgcgacgctt cttgtgttca ggcgagtgtg cgcccgcctg acggattgcg
     1201 ccaccaagcc ggtcagggtg gtggtacaag ggttcaggtg gcgcgggcgt tcttctgatg
     1261 cggcgaatga cgcacttagg ccgttcgggc gaaatcaccc gaaatcgcct gtgacgcttg
     1321 aagttgtgtt gcggctgccg gggcgaaacc acgccggcct tcgtcatttc acagcgtctt
     1381 gaacggatca tccagaagca cgactggcat catggcgtgt gccaacgcac gcttctatcg
     1441 aaggcccggg atcattcaac gctccgggga gcgcaagaaa ggtctgagat ggcaagaagg
     1501 ttttcgacgc gaattttggc gatcgcagcg tccggcctgc tggcgtcgca cccggtacgg
     1561 gtatcggcgg cgcccccggc cggtgccgac tctgatctcg cgccttggta tcgaagcctt
     1621 cgtcagccgc agaccaacct gtcctgctgc gaccgctcgg attgccgaac cgtccggtat
     1681 cgtatcgtgg acggtcattt tcaggctttt atcggcagcg agttcgcgcg ctggcaaaac
     1741 ccgccctacg cttgggtcga tgtgccggaa aacagcgttt tgcacaggca tgacaatccg
     1801 acaggcgagg gggtcgcctg ctgggcaggc gcgcagatca tctgtttcat cgaaggcacc
     1861 ggcgcttgat gcctttgggc cggagtcgga accgaatccg aatccgaccg tctgagagaa
     1921 gttgctcaga atcgcgccga caccgcttgc gcggttgatc aattgcgcgg caatagtggc
     1981 cggtacatcc ttgacgtaac cgaaacgttc aggctgtggt cggcggattt catcgtgacc
     2041 gtgggattgc tgatcagcgt cgattaaggt gcgaaggccg tgtccgactg cgggcaaaaa
     2101 agacggcggc ccgggcatgg ctggttcaga aacgtcgcga aaggagtgtc gaaaatgcgg
     2161 atggcccggc ttggcgccat agatctatcg accgccctga ccggcctgct ggccgcgggc
     2221 atctgctacg cggcctggct aacctcgatc gtgttctgcg ccgcccatgg cgctcggccg
     2281 ttgctcata
//
LOCUS       DASWQN010001118         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1296791, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001118 DASWQN010000000
VERSION     DASWQN010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296791"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VGC82_19125"
     CDS             complement(<1..404)
                     /locus_tag="VGC82_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0425438.1"
                     /translation="MPITIRVKAPRKRVRKGDLVKATLVDRPRPAGKSSDLNIARLPE
                     EVSFKTKAYAALKQAITDLDIYQSSGPVMLDERELSERLGVSRTPVREAVAVLQQEGF
                     LKVVPRRGIMVVRRTKREIIEMIQAWAALESMA"
     gene            complement(726..1199)
                     /locus_tag="VGC82_19130"
     CDS             complement(726..1199)
                     /locus_tag="VGC82_19130"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEY0425439.1"
                     /translation="MKGQPGRGTLEKEKPPIFGMVQRGGAVVLHLLANVQQPTIKPLV
                     LAGVASGSLINTDEYAIYSRLLEWGFGHKAVNHGRGEYARDEDGDGFCEVHVNTMEGV
                     WSLLRSWLRPHRGISQEKLPCYLALFEFVHNARCRGKALLGALLAALLPTTPKPT"
     gene            complement(1196..1681)
                     /locus_tag="VGC82_19135"
     CDS             complement(1196..1681)
                     /locus_tag="VGC82_19135"
                     /inference="COORDINATES: protein motif:HMM:NF024170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEY0425440.1"
                     /translation="MESLVNIQGLIDDARCFETVRHLRWPEGVRCPQCGSDKVARHGH
                     DERQRDRQRYRYPACQVRFDDLTDTIFAGHHQPLRLWILCLYFMGLNLSNQQIARELD
                     LNVGDVQAMTEQLRTGILAARPAEVLEGVVECDEVYVTAGHKGQPVAVAKKAGRAGAA
                     D"
BASE COUNT          355 a          633 c          552 g          365 t
ORIGIN      
        1 gccatgctct cgagcgcggc ccaggcttgg atcatttcga tgatctcgcg cttcgtccgg
       61 cgcaccacca taatgccccg ccgcggaacc actttcagaa atccctcttg ctgcagcaca
      121 gctacagctt cgcgaacggg cgtacggctg acacccaggc gctccgacaa ctcgcgttcg
      181 tccagcatca caggcccgga tgactggtag atgtcgaggt ctgtgatcgc ctgcttcaga
      241 gctgcatacg cctttgtctt gaagctcacc tcttcgggaa ggcgagcaat gttcagatcg
      301 ctgctctttc cggctggacg aggccggtcg actagtgtcg ctttcacgag atcgcctttg
      361 cgaacccgct tgcgcggggc ttttacgcgg attgtaattg gcataccaaa tacctcgtgt
      421 caagatattt cttcaacgtt gtcggtgcac cagtgctgct gcggcagagt ctagcatgcc
      481 atagcctggt atgccagctc cgagaacacc gacgctttgg cagtgcgcga gcgcctcgca
      541 tttcgccgtc tgctgagttg cccaccggac gaagcctgac ccgccgcgaa tggaacaggc
      601 atggtcggtt gcggtgtcgg cgttgaggtc ggttcagtct tggtgccgcg agcccgtgcc
      661 atgctagcgt aaggcaacca cgccagcgcg tacccgaccg ctcccgcgat caaaaccgcg
      721 aggctctatg tgggtttcgg ggtggtgggc agcagggccg ccaagagggc accgagcaag
      781 gccttgccgc ggcacctggc gttatgcacg aactcgaaga gggcgagata acagggcagc
      841 ttctcctgcg agatgccgcg gtgcggccgc agccaggagc gcaacagcga ccaaacccct
      901 tccatcgtgt tcacgtggac ctcgcagaag ccgtcaccat cctcgtcacg agcgtactca
      961 ccccggccgt ggttcaccgc cttgtggccg aagccccact caagcagccg gctgtagatt
     1021 gcatactcgt ctgtgttgat caggctgccc gaggccacgc cggccaggac caacggcttg
     1081 atcgtcggtt gctgaacgtt ggccagcagg tgcaggacca cggcgccgcc gcgctgaacc
     1141 atgccgaaga tcggcggctt ctccttctcc aaagtgccgc ggcccggttg gcctttcaat
     1201 ccgcggcgcc ggcccgaccg gcttttttcg ccaccgccac cggctgacct ttatggcccg
     1261 ccgtgacgta gacctcgtcg cattccacca cgccctccag cacctcagcc gggcgtgccg
     1321 ccaggatccc ggtgcgcaac tgttcggtca tggcttgtac gtcaccaacg ttgagatcga
     1381 gttcccgggc gatctgttgg ttcgacaggt tcaggcccat gaagtagagg cacaggatcc
     1441 agagccgcag cggctgatga tggccggcga aaatggtatc ggtgaggtca tcgaagcgca
     1501 cctgacaggc cgggtaccgg tagcgctgcc ggtcccgctg ccgctcgtca tggccatgtc
     1561 gcgccacctt gtcgctcccg cattgcgggc accgcacgcc ctcgggccag cgcagatgcc
     1621 gaaccgtctc gaagcaccgg gcatcatcga tcaggccctg gatgttgacc agactctcca
     1681 tcgcgaccga tcccttcccg ctggtaaccg tggccgcccg aaacggcaaa accgctcctc
     1741 gggctcatcg gccatctcaa caggcggcga ccacgatccg cgccccagct tggcgcccac
     1801 caccccgaaa cgcgatatga gccaacggct ttaccgaaaa aaactatttc tacaaagcaa
     1861 atatggtgtt gggaagaatc tttaaaaccg agcatatcct ctctt
//
LOCUS       DASWQN010001119         5436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2483021, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001119 DASWQN010000000
VERSION     DASWQN010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2483021"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            568..1920
                     /gene="miaB"
                     /locus_tag="VGC82_19140"
     CDS             568..1920
                     /gene="miaB"
                     /locus_tag="VGC82_19140"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY0425441.1"
                     /translation="MTETERKRLHVITWGCQMNVYDSGRMADVLAPLGYRPVTEPDGA
                     DMVILNTCHIRDKAAEKVFSELGRLRLMKEAGGGRMILAVAGCVAQAEGAEIFARAPY
                     VDIVLGPQTYHRLPEMVARASRAGGKVIETDFPPEDKFDHLPNSATPQGVTAFLTIQE
                     GCDKFCSFCVVPYTRGAEQSRAAITILREARHLISQGAREITLLGQNVNAWHGAAPDG
                     SIWGLGRLLRALSELPDLLRLRYATSHPRDMDDDLIDAHRDLPKLMPFLHLPVQSGSD
                     RMLAAMNRKHTADDFRRIVGRLRDARPDLALSSDFIVGHPGETEADFEATMALVRDIK
                     FAMAYSFKYSARPGTPAAGAPGQVPEHVKDQRLRMLQGLLRDQQIAFSAGCVGLDLPV
                     LFTGPGRHPGQIAGRSPFLQPVHLTANRDLIGTESTVRIVANHPNSLAGTLVQERRIA
                     "
     gene            1917..2960
                     /locus_tag="VGC82_19145"
     CDS             1917..2960
                     /locus_tag="VGC82_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY0425442.1"
                     /translation="MTQLAAPQAPPAPAASGGKAITLQFNDNTLLPLLLGDHDRHLVR
                     IEQALGIRLSCRGNRIAIAGDASRVDIAQEMLRALWRRLEQGESVGRSEIETAIRLAD
                     AENDPRLPLSDIPAIRTRRGAVGPRSPGQITYMEALATHEMVFGVGPAGTGKTFLAVA
                     QAVAMLQAGKVDRIVLSRPAVEAGERLGFLPGDMKEKIDPYLRPLYDALHDMMPADQV
                     IRRMTNGEIEVAPLAFMRGRTLAHCYAILDEAQNTTPMQMKMFLTRMGEGTRMVVTGD
                     LSQVDLPNGIRSGLRDALDTVEGIRGIGVVRFEQRDVVRHPLVARIVGAYDQRDAAQS
                     EARRERERPQTSE"
     gene            2971..3423
                     /gene="ybeY"
                     /locus_tag="VGC82_19150"
     CDS             2971..3423
                     /gene="ybeY"
                     /locus_tag="VGC82_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY0425443.1"
                     /translation="MEPPGSRPPDLDGHTVDVVVADPAWNRLIPNAGTIVRRAAHAAG
                     MEGTVVLSSNRVVRGLNGRHRGKNKPTNVLTYEHPAPEIILALGVVRAEAAAQGRRPG
                     HHLAHLVVHGALHLNGEDHDHPGEARRMEMAEARILHRIGVPNPWKRT"
     gene            3420..4307
                     /locus_tag="VGC82_19155"
     CDS             3420..4307
                     /locus_tag="VGC82_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY0425444.1"
                     /translation="MSGHKGTLLSRLGYLLGRKQAEHSLRDSIAELVQEAADAQQGSG
                     EAPELDRHERLLIANVLRLRETTADDVMVPRADIVAMRADVTLGQAIELIRNDGHSRL
                     PVYREQLDDVIGMVHIKDVFAYVGRPEAFSLEAIMRKPLLVAPQIPVLDLLLQMRMQR
                     MHLALVVDEYGGIDGLVTIEDLVETITGDIADEHDDVEAPMISERPDGAMDLNARMPI
                     EDFEEKVGSILTEDERDADIDTVGGLVFTLAGRVPTRGEVISHPSGIEFRVLDADARR
                     IRRLRARRTEALPKPAAAA"
     gene            complement(4388..4813)
                     /locus_tag="VGC82_19160"
     CDS             complement(4388..4813)
                     /locus_tag="VGC82_19160"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0425445.1"
                     /translation="MKEVVVDASVAAKWVVDEEYSTEAASLLQYDARHAPDHWQAEAV
                     NVLWSKVFRGDLTAPDAHERMMVLLRAPVVAAAITDLLPRAFAISVGNAVTIYDSLYI
                     ALAEKRDIPFVTADQRLIRRISGDAGLAKRMIWVGGLSG"
     gene            complement(5126..>5436)
                     /gene="fbiC"
                     /locus_tag="VGC82_19165"
     CDS             complement(5126..>5436)
                     /gene="fbiC"
                     /locus_tag="VGC82_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619203.1"
                     /note="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase; catalyzes radical-mediated transfer of
                     hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to
                     5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form
                     7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO); functions
                     in F420 biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase"
                     /protein_id="HEY0425446.1"
                     /translation="ITNIQTSWVKMGPEGAAMCLNAGANDLGGTLMNESISRAAGTQH
                     GQEFPPEAMERLIGSLVRTPVQRDTLYRPVGNERRVVSFNAAELAPVVQTPPRKVMAE
                     "
BASE COUNT         1021 a         1726 c         1732 g          957 t
ORIGIN      
        1 tatggcgttt ggaccggcag ggtgccgtcg gaccgcatga tagaccgggt tatccggtcg
       61 aacgagggtg ctgcggggtc atgaccggaa gttacttgga gtctccggtt cccattccga
      121 atattgataa actgacgaga accacgcacc gccgtggtct cgcggatgca cgacgttcgt
      181 ttgcctggtg gaaagcaaag caaggtcggc aaaaacttgc cccggcgctc cgcttcctcg
      241 tgaggttacg ggacaggctt tacccgcgga cgcccgcccg cggccgttca cggggcatgg
      301 ccaccctaat gaaatcggtc aaacaggcct tgctggtgtg cgcgcctgcg gcactccatg
      361 tatcttccat gacatcacgc cgagttgttg aaagaaccgg atggatactg ccaaactaca
      421 gccaccccag actacagcca cccaaggagg caatcacgcg ctgtcgggct caaccaagcc
      481 gttctgcttg accgtatcgg tccagccgcc catctctccc gctccccgcg ccggaaccgc
      541 ccggctgggt cccgcggagc atttcctgtg accgaaaccg agcgcaagcg cctgcacgtc
      601 atcacctggg gctgccagat gaacgtgtat gacagcggcc ggatggcgga cgtgctcgcg
      661 cccctcggct accgccccgt gacggaaccg gacggcgcgg acatggtgat cctcaacacc
      721 tgccacatcc gcgacaaggc cgcggagaaa gtgttctccg aactcggccg tttgcgcctg
      781 atgaaggagg ccggtggcgg acggatgatc ctggccgtcg ccggctgcgt cgcccaggcc
      841 gaaggtgcgg aaattttcgc acgcgcgcct tacgtcgata tcgtgctcgg tccgcagacc
      901 tatcatcgcc tgccggagat ggtcgcccgc gccagccgag ccgggggcaa ggtcatcgag
      961 acggacttcc cgccagagga caagttcgat cacctgccga acagtgccac gccgcaaggc
     1021 gtgaccgcct ttctcacgat ccaggaggga tgcgacaaat tctgcagctt ctgcgtcgtc
     1081 ccctacacga ggggcgccga gcaaagccgc gcagccatta caatcttgcg tgaagcgcgc
     1141 cacctgattt cccaaggcgc ccgcgagatc accctgctcg ggcaaaacgt caacgcctgg
     1201 catggcgcgg caccggacgg ctcgatctgg ggccttggac ggctgctgcg cgcgctgtcg
     1261 gaactccctg acctgctgcg gctgcgctat gccacttcgc acccgcggga catggacgac
     1321 gatctgatcg acgcgcaccg cgacctgccc aagctgatgc cgttcctgca tcttccggtc
     1381 cagtccggtt ccgatcgtat gctggcggcg atgaaccgca agcacaccgc cgacgatttc
     1441 cgccgcatcg ttggaaggct gcgtgatgcc cgacctgacc tggcgctgtc ttcggacttc
     1501 atcgtcggcc atcccggaga gaccgaagcc gacttcgaag ctacgatggc tttggtgcgg
     1561 gacatcaaat tcgccatggc ttacagcttc aaatactcgg ctcgtcccgg cacgccggcg
     1621 gcgggcgcgc ccggtcaggt gccggaacat gtgaaggacc agcggctgcg gatgctgcaa
     1681 ggattgctgc gcgatcagca gatagcgttc agcgcggggt gcgtcgggct cgacttgccc
     1741 gtgctgttta ccggcccggg gcgccacccc gggcaaatcg ccgggcgatc gccatttttg
     1801 caaccggtgc atctcacagc caatcgtgat cttattggta ccgaatcgac tgtgcgaatc
     1861 gtagcgaacc acccaaactc attagcggga acccttgtcc aggagagacg catcgcttga
     1921 cacagctcgc cgccccccag gcaccgcctg cccccgccgc atccggcggt aaggccatca
     1981 ccctccagtt caacgacaac accctgctgc ccttgctttt gggcgaccac gaccggcatc
     2041 tggtgcggat cgagcaggcg ttggggatca gactgtcttg tcgaggcaac cgaattgcca
     2101 ttgcggggga tgcctcgcgt gtcgatatcg cgcaggagat gctgcgggcg ctgtggcggc
     2161 ggctggaaca gggtgaaagc gtgggaaggt ctgaaatcga gacagcaatc cgcctggccg
     2221 acgcggaaaa cgatccgcgt ctgccgctgt ccgacatacc ggcgatccgc acccgccgtg
     2281 gtgcggttgg ccctcgcagc ccgggccaga tcacctacat ggaggcattg gcgacgcacg
     2341 agatggtgtt cggcgtcgga cctgccggca cgggaaaaac ctttcttgcc gtagcccagg
     2401 ccgtggcgat gctgcaagcg ggcaaggtcg atcggatcgt gctgtcccgg ccggcggtcg
     2461 aagccggcga gcggctcggt ttcctgccag gcgacatgaa agagaagatc gacccctatt
     2521 tgcgcccgct atacgatgcg ctgcacgaca tgatgccggc cgatcaggtc attcgccgca
     2581 tgacgaacgg agaaattgag gtagcgcccc tggcgttcat gcgtggccgg accctggcac
     2641 attgctacgc gatcctggat gaggcgcaga acaccacgcc catgcagatg aagatgttcc
     2701 tgacccgcat gggcgagggg acccggatgg tggtaaccgg cgacctcagc caggtggacc
     2761 tgccaaacgg catccgctcc ggcctgcgcg atgctttgga cacagtcgaa ggcatccgcg
     2821 gcatcggagt cgtacggttc gaacagcgcg acgttgtgcg acatcccctg gtggcgcgta
     2881 tcgtcggggc ctatgatcag cgcgatgccg ctcaaagcga ggcgcgacgg gagcgggaac
     2941 gtccgcaaac gagcgagtaa ttggattctg atggaacctc ctggtagccg gccgcccgat
     3001 ctcgatggtc ataccgtcga tgtcgtggtg gctgatccgg cttggaacag attgataccg
     3061 aacgccggga cgattgtccg tcgagccgcg catgccgccg gcatggaggg gaccgtcgtc
     3121 ctgtcgtcca accgcgtcgt gcgcgggctg aacggccgcc atcgcggcaa gaataagccc
     3181 accaacgtcc tgacgtatga gcatccggcg cccgaaatca tcctggcgct gggcgtggtc
     3241 cgggcggagg cggctgccca aggccgtcgt cccgggcatc acctggcgca tctggtggtc
     3301 catggcgcgt tgcacctgaa cggcgaagat catgaccatc ccggcgaggc ccggcggatg
     3361 gagatggccg aggcgcggat cctgcaccgg attggcgtac ccaacccctg gaagcgcaca
     3421 tgagcgggca caaaggcact ttgctcagcc ggctcggtta cctgctgggg cgcaagcagg
     3481 ccgaacattc gctccgcgat tcgatcgccg aactggtgca ggaagccgcg gacgcgcagc
     3541 agggctcagg cgaggcgccg gaactcgatc gacacgagcg cctgctgatc gcgaatgtcc
     3601 tgcggctgcg ggaaaccacg gcggatgacg tgatggttcc ccgagccgat atcgtggcca
     3661 tgcgggccga cgtgacgctg gggcaggcga tcgaactgat ccgcaacgac ggccactcac
     3721 ggttgccggt gtaccgggag cagttggacg acgtgatcgg catggtccac atcaaggacg
     3781 tgtttgccta tgtcggccgc ccggaagcgt tttcgctgga ggcgatcatg cgcaagccgc
     3841 ttctggtggc gccgcaaata cccgtcctgg acctgctgct gcagatgcgg atgcagcgca
     3901 tgcacctggc gctggtggtg gacgaatatg gcggcatcga cgggctggtc acgatcgagg
     3961 atctggtcga aaccatcacc ggcgacatcg cggacgagca cgacgatgtc gaagcgccga
     4021 tgatcagcga acggcccgac ggtgccatgg acctgaatgc ccggatgccg attgaggatt
     4081 tcgaggaaaa agttggctcc atcctgacgg aagacgaacg cgacgccgat atcgatacgg
     4141 ttggcggtct cgtgttcacg ctggccggcc gggtgccgac acgaggcgag gtgatcagcc
     4201 acccctcggg catcgaattc cgtgttttgg acgccgatgc cagacgcata cggcgactgc
     4261 gcgcccgccg gaccgaggcg ctgccgaaac ctgccgccgc ggcgtagcgg tgatcccgct
     4321 gaacgtttga tctttctcgc cagcgaagac gggcttacgg gggttttgcg tgggaagagc
     4381 cggtgcgtca gcctgacagg ccgcccaccc agatcatgcg cttagccaaa cccgcatcgc
     4441 ctgagatccg ccggatcaac cgctggtcgg ccgttacaaa cggaatatcc cgcttctcag
     4501 ccagtgcgat gtacagcgaa tcatagattg tcaccgcgtt gccgacggaa atcgcgaagg
     4561 cgcgggggag caggtcggtg attgccgccg caacgaccgg agcgcgcaac agcaccatca
     4621 tgcgttcatg cgcgtccggc gcggtcagat cgcctcgaaa taccttcgac cataggacat
     4681 tgactgcttc cgcctgccag tggtctgggg catggcgcgc atcatattgc aacaggctag
     4741 cggcctcggt gctatattcc tcatcgacaa cccatttcgc ggcaaccgag gcatcgacga
     4801 caacctcctt caccggtcat tgtcagcgtg atcgcggtcc cgatcctgcc ggatcagagt
     4861 ggtactgtcg gttgactgcg ggggcgtgcg agcccgcaaa cgcgcagcgg cctcggcgaa
     4921 ggtttccgtc tcggcttgaa gggcttgttc gaggatcatg cgatgctcgg cctcagctga
     4981 acgcccaacg cgggcagccc gttgcttcag cgtctggata agcctgtcat caaggctgcg
     5041 gactagaagc tggcccacgg ggtccctcct ttgttgtgct atcaagcgat gataacacaa
     5101 cgaagggaat cgtcaatagc tttggctact ccgccatcac cttgcggggc ggagtctgca
     5161 ccaccggcgc aagctcggcc gcattgaagg ataccacgcg ccgttcattt cccacgggcc
     5221 ggtacagcgt gtctcgctgt accggcgtgc gaaccagcga gccaatcaac cgttccatcg
     5281 cttcgggcgg aaattcctga ccatgctggg tgccggcagc gcgagaaatg ctctcgttca
     5341 tcagcgtgcc acccaggtcg ttggcaccgg cgttcaggca catcgcggca ccttcgggtc
     5401 ccatcttcac ccaggaggtc tggatgttgg tgatca
//
LOCUS       DASWQN010001120         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1691792, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001120 DASWQN010000000
VERSION     DASWQN010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691792"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1348)
                     /gene="nrfD"
                     /locus_tag="VGC82_19170"
     CDS             complement(8..1348)
                     /gene="nrfD"
                     /locus_tag="VGC82_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY0425447.1"
                     /translation="MTAVEPVVLPGSNNPDMTDHVSAITLREKAFHWWWIAIAPPAAL
                     TSVLIGSVLYLFYAGVGIWGIDWPVAWGFAIINYVWWIAIASGGTFISALFFLTRSEW
                     RNSINRVAESMTLFGAACAGIYPILHLGRPWFFYWLFPYPNSMNLWPQFRSPLLWDFF
                     AILAYVIASVLFWYFGLLPDLATLRDRASTRKKQLIYGAMALGFRGTGKQWGHYHALY
                     GVLAAVMAPLVCSVHSIVGLDFAGAQTVGWHSTIFPPFFIFGAFLSGFATVLLLIMPL
                     RRLLRLQRYITTRHFDVLGRLLLTSSLFVGYAYIMDGFSTFYGGEKAEIVNYSERLFG
                     YYAAIFWATVLFNIVLPQLLWFRAIRLNQVVVALISIGVIIGMWCERYTIVVVALHRP
                     RLPSSYGIYHGSIWDWLTMMGTVGFFIFGILLIVRLIPVISMFEMREILAPRRP"
     gene            complement(1345..>2810)
                     /locus_tag="VGC82_19175"
     CDS             complement(1345..>2810)
                     /locus_tag="VGC82_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY0425448.1"
                     /translation="QPQARPLYDGISPHYLLALFAGPEERDSRDIVRQTHRLNDEQWH
                     DALAAGVVPNTHALVSTIALKPDRPAAGRGADAGAETVPLTLLFRPDPYIWDGRLANN
                     AWLQELPRPLTKLTWDNPLLVSPATAKQLKLVNGDRVSVSVGDANVTLPAWIVPGQAA
                     DCAVALLGFGRLDVGMVGKGVGYDLYPLMAASGPVALRKAEGREVLATTEHHDPIFSG
                     AGDYVRRGTLSEFKADPAFLGKPPEQPKLYERKPTGPAAWGMSIDLNACIGCNACVVA
                     CMAENNIPTVGKAQVIHEREMHWLRIDRYYEGSPDSPDMLFQPMLCQHCEQAPCEIVC
                     PVGATVHDEEGLNVMVYNRCVGTRFCSNNCPYKVRRFNYFAFAQEEHRPAVARNPEVT
                     VRARGVMEKCTFCLQRIAAARIDADAANRPVGGNEVKTACQAACPTQVFTFGNMAEGG
                     DVVERKKSPLTYALLDDLNTKPRVTYEARISNPGTAA"
BASE COUNT          584 a          871 c          897 g          458 t
ORIGIN      
        1 aaaagcttca tggacgtcgc ggtgccagga tttcgcgcat ctcgaacatc gagataaccg
       61 ggatcaggcg tacgatcagc aagatgccga agatgaagaa tccgacggtg cccatcatcg
      121 tcaaccagtc ccagatggag ccatggtaga tgccgtagga ggacggcaga cggggacgat
      181 gcagggcgac gacgacaatg gtgtaccgct cgcaccacat gccaatgatg acgccgatgg
      241 agatcaaggc gacgaccacc tggttcagtc ggatggcgcg gaaccagagc aactggggca
      301 gcacgatgtt gaacaggaca gtcgcccaga agatagcggc gtagtaaccg aacagccttt
      361 cactatagtt tacgatctcc gctttttcgc cgccgtagaa ggtggaaaac ccgtccatga
      421 tgtaggcgta tccgacaaac aggctgctgg tgagcagcaa gcgccccagg acatcgaaat
      481 ggcgggtcgt gatgtaacgt tgcagtcgaa gcaggcggcg cagcggcatg atcagcagta
      541 ggacagtcgc gaagccggac aggaacgcgc cgaagatgaa gaacggcgga aaaatcgtcg
      601 agtgccagcc gacggtctgg gcacccgcga aatccaggcc gacaatgcta tgcaccgaac
      661 agaccagcgg cgccatgacc gccgcgagca cgccgtacag ggcgtggtag tgaccccact
      721 gcttgccggt gcctcggaaa cccagcgcca tggcaccata gatcaactgc tttttccggg
      781 tggatgcgcg atcgcgcagc gtcgccagat cgggcagcaa gccgaagtac cagaacagca
      841 ccgaggcgat gacataggca aggatcgcga agaaatccca cagcaagggg ctgcggaact
      901 gcggccacag gttcatgctg ttgggatacg ggaacaacca gtagaagaac caggggcggc
      961 caagatgcag gatcggatag ataccggcac aggcggcccc gaacagagtc atcgactcgg
     1021 caacacgatt gatcgagttg cgccactccg accgggtcag gaagaacagc gccgaaatga
     1081 acgtgccgcc actggcaatg gcaatccacc agacgtagtt gatgatcgcg aaaccccagg
     1141 cgacgggcca gtcgatgccc cagatgccga cgccggcata gaacagatac aggaccgagc
     1201 cgatcagcac gctggtcagc gccgccggcg gcgcaatggc gatccaccac cagtggaagg
     1261 ccttttcccg caacgtgatg gcggagacat ggtcggtcat gtcggggttg ttgctgcccg
     1321 gcagcacgac gggttcgacc gccgtcatgc cgcggtcccg ggattgctga tgcgcgcctc
     1381 gtaggtgacg cgcggcttgg tattcaggtc atccagcagc gcgtaggtca gcggactttt
     1441 cttgcgttcg acgacgtccc cgccttccgc catgttgccg aaggtgaaga cctgcgtagg
     1501 acatgccgcc tggcaggccg tcttcacctc gttgccgccg accggccggt tggcggcgtc
     1561 cgcatcgatc cgcgcggcgg cgatgcgctg caggcagaac gtgcattttt ccatcacccc
     1621 gcgggcacgg acggtcacct cgggattgcg cgcgaccgcc ggacgatgtt cctcctgggc
     1681 gaacgcaaag tagttgaagc gccggacctt gtaaggacag ttgttggagc agaaccgcgt
     1741 gccgacgcag cggttgtaca ccatgacgtt caggccctcc tcgtcatgca ccgtggcgcc
     1801 gaccgggcag acgatctcgc atggcgcctg ctcgcagtgc tggcacagca tcggctggaa
     1861 cagcatgtcg ggggaatccg ggctgccctc gtagtagcgg tcgatacgca gccagtgcat
     1921 ttcccgctcg tggatcacct gtgccttgcc gaccgtcggg atgttgtttt ccgccatgca
     1981 ggcgaccaca caggcgttgc agccgatgca ggcgttgagg tcgatgctca tgccccaggc
     2041 agccggaccg gtgggcttgc gctcgtagag cttcggctgt tccggcggtt tgccgaggaa
     2101 cgccggatca gccttgaact cggacagcgt tcctcgccgc acgtaatctc cggcaccgga
     2161 aaaaatcggg tcgtggtgtt ctgttgtcgc cagcacttcg cggccttcag ccttgcgcaa
     2221 ggccaccggt ccgctcgcgg ccatcagcgg atacaggtcg tagccaaccc ccttccccac
     2281 catgcctaca tccagtcggc cgaagcccag caatgccacg gcgcaatcgg ccgcctggcc
     2341 cggtacgatc caggccggca aggtgacatt cgcgtcgccg acggagactg aaacacgatc
     2401 gccgttgacc agcttcaact gcttggcggt agccggagag accagcagcg ggttgtccca
     2461 ggtcagcttg gtcaacggcc gcggcaactc ctgcagccag gcattgttgg cgagccggcc
     2521 atcccagatg tagggatccg gccggaacag cagggtcagc ggaaccgtct cggctcccgc
     2581 gtccgcccct cgaccagcag cgggccgatc cggcttcagc gcaatcgtgc tgaccagtgc
     2641 gtgggtgttc ggcaccacgc ccgccgccag cgcatcatgc cattgctcgt cgttcaaacg
     2701 atgggtctgc cgcacgatgt cgcggctgtc gcgttcctcg ggaccggcga acagcgccag
     2761 caaatagtgc gggctgatcc catcgtagag cggccgagcc tggggctgca
//
LOCUS       DASWQN010001121         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1956178, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001121 DASWQN010000000
VERSION     DASWQN010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956178"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VGC82_19180"
     CDS             <1..914
                     /locus_tag="VGC82_19180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0425449.1"
                     /translation="HDIGSPVKAGQVLATIDAPDLDQQLSQARATLASVKANEQIATL
                     TANRNNILLQKQIVAQQLADQTTADAAAKKAVVDANEANVKQLEAMQSFKTLAAPFDG
                     VVTARNVELGMLINSGGSGQPLFEVSDLHRVRIFVQVPQSFSAGLRPGVKAKFEMPQY
                     PGVQFDAVLTHTSKAMNATSHSMQVQLQAENSAGKFFGGSYCNVHFEIPANPNLVTVP
                     STALVTSNQGSQVATVDGNGKIAFKSLQLGRDLGDSVEVLAGLSPSDRVVNNPPETVA
                     AGDTVHVAAAAPQPQPAANAPAATSRQ"
     gene            925..>1593
                     /locus_tag="VGC82_19185"
     CDS             925..>1593
                     /locus_tag="VGC82_19185"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEY0425450.1"
                     /translation="MRICANLPPGGRDLRLDLFRGLANWAIFLDHIPHEVLNSITTRN
                     YGFSDAADLFVFISGYTAALVFGRIMIEQGYVATASKLAKRASVLYAAHITVLIAFIV
                     AVASVSRGLHDPDDLNQFNIGAFMSKPIWEFIQALMLRYKPVNLDVLPLYILLLATFA
                     PVLWLMVRKPTLALVCSMTVYFAGRHFGWNLSGSPAGLWYFNPFAWQLLFVLGAWIAV
                     GGAQS"
BASE COUNT          301 a          501 c          470 g          321 t
ORIGIN      
        1 accacgacat cggatcgccg gtgaaggccg gacaggtgct ggccaccatc gacgcgcccg
       61 atctcgatca gcagctcagc caggccaggg cgacgctggc gagcgtcaag gccaacgagc
      121 agatcgcgac gctgaccgcg aaccggaaca atatcctgct gcagaagcag attgtcgcgc
      181 agcaactcgc ggaccagacc actgccgacg ccgcggcgaa gaaggcggtg gtcgacgcca
      241 acgaggccaa cgtaaagcag ctcgaggcca tgcaatcgtt caagacgctc gccgcgccgt
      301 tcgatggcgt ggtaaccgcg cgcaacgtgg aactcgggat gctgatcaac tcgggcggtt
      361 caggccagcc gctgttcgag gtttcggacc tgcaccgcgt tcgcatcttc gttcaggtgc
      421 cgcagtcgtt ttcggccggg ctgaggccag gggtaaaggc caagttcgaa atgccgcagt
      481 atcccggcgt gcagttcgac gcggtgctca cgcatacctc caaggccatg aatgcaactt
      541 cgcacagcat gcaggtccaa ctccaggccg agaattctgc cggcaagttt ttcggcggca
      601 gctactgcaa tgtgcatttc gaaatcccag ccaatccgaa cttggtgacc gttccatcga
      661 cggcgcttgt gaccagcaat caaggcagcc aggtcgcgac agtcgacggc aatggcaaaa
      721 tcgccttcaa gagcttgcaa ctcggccgcg accttggtga cagcgtcgag gttcttgctg
      781 gcctgtcgcc gtcggaccgg gtcgtgaata acccgccaga gaccgtggcg gcgggcgaca
      841 ccgttcacgt cgctgccgca gcgccgcagc cgcaacccgc ggcgaacgca ccggcagcaa
      901 cctcccggca atgaggcgcg tccgatgcga atttgcgcaa atcttccgcc cggcggccgc
      961 gacctacggc tggatctgtt tcgcggcctg gccaactggg cgatcttcct cgatcacatt
     1021 ccgcacgagg tgctgaattc gatcaccacc cggaattacg gcttcagcga cgcagccgac
     1081 ctgtttgttt tcatctccgg ctatactgct gcgctcgtat tcggacggat catgatagag
     1141 cagggatatg ttgccaccgc gtcgaaattg gcaaagcgcg cgtcagtcct gtacgcggcc
     1201 cacatcacgg tgctgatcgc tttcatcgtc gccgtcgcga gcgtttcccg cgggctccat
     1261 gatcccgatg atttgaatca gttcaacatt ggcgccttca tgagcaagcc gatctgggaa
     1321 ttcatccagg ccctgatgct gcgatacaag cctgtcaatc tggacgtgct tccgctttat
     1381 atcctcttgc tggcaacctt tgctcctgtc ctgtggctga tggtccgcaa gccaacgctg
     1441 gcattggtct gttcgatgac ggtatatttt gccggccggc attttggctg gaatctttca
     1501 ggctctccgg cgggtctctg gtatttcaac ccgttcgcct ggcagctgct gtttgtgctg
     1561 ggcgcctgga tcgccgttgg cggcgctcaa tcc
//
LOCUS       DASWQN010001122         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_504055, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001122 DASWQN010000000
VERSION     DASWQN010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504055"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VGC82_19190"
     CDS             <1..303
                     /locus_tag="VGC82_19190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425451.1"
                     /translation="EPSLAVHGVPIPANLLANHAVFPPVSPQHAIVAGQLVEREIHPS
                     SDLRLLAAGRSNNVSCCFCQPISILQHFIANEAPLARRYLASFLPASLFTRRQSSE"
     gene            complement(278..529)
                     /locus_tag="VGC82_19195"
     CDS             complement(278..529)
                     /locus_tag="VGC82_19195"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY0425452.1"
                     /translation="MPKVVLSSGSVGQQFTGGQTEFEVEATTFRRLVLELDRRFPGLG
                     KQVEESMAIAIDGEIFQDAYAAELKPGSEIYLIPKIGGG"
     gene            complement(643..>2757)
                     /locus_tag="VGC82_19200"
     CDS             complement(643..>2757)
                     /locus_tag="VGC82_19200"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY0425453.1"
                     /translation="ARIVSIDTKKAEALKGVFAVVTAADFPDIPSEEAFVGEGPMNFR
                     DLALNCMARTKVLYEGHALAAVAASTQAIADEALALIDVNYEILPHVIDVEEAMADGA
                     PILHDDMFTTGVDPKPTKPSNIAKVVTFRKGDAEAGFKEADVIVEGRYTTQPVHQAYI
                     EPHACLATYSPDGQVSIHSSSQGHFMVRAYTAKLLGMNMANLRVNPAEIGGGFGGKTL
                     VYLEPVAVALSKKSGRTVKMQMTREDVFRASGPTSGAAMEVKIGAKKDGTIVAAKQVL
                     KYQAGAFAGSPIGPGCMCGFAMYDLPNVDVVGYDVVSNRPKVAAYRAPGAPITSFAVE
                     SLMDELAIKLGIDALTLREKNAAKNGTKTHYGPTHQNIGFTAVLDAVKSHPHWKSPLK
                     PGHGRGLATGFWFNIGGESTAQVHVNEDGSVTAATGSPDIGGSRASIGMMVAEVLGVP
                     AATVRTIVADTASIGFTHVTGGSRVTFATGMAATQAAEKIVDDLKKRAALTWDIPVEA
                     VEWKDGKAYPAGSNAGAFEPLDLAALALKAARTGGPLIAEVSLNAQGAGPGFGAHICD
                     VSVDKETGHVKIERYTAIQDVGKAIHPSYVEGQIQGGAAQGIGWALNEEYIYNTKGQM
                     ENAGFLDYRVPVASDIPMIEAVLVEVPNPRHPFGARGVGEVPIVPPMAAIGNAIRSAT
                     GLRMADLPMSPPKVRAALDARG"
BASE COUNT          487 a          951 c          768 g          551 t
ORIGIN      
        1 gagccatctt tagcggtgca tggcgtaccc attccagcaa atcttcttgc gaatcacgct
       61 gtctttccac cagtcagccc ccagcacgct attgttgccg gccaactggt ggagcgagag
      121 atccatccgt cttccgacct ccgcctgctg gcggccggtc gaagcaataa tgtgtcctgc
      181 tgcttttgcc aaccaatatc aatattgcaa cattttatcg ccaacgaagc tccgctcgca
      241 aggcggtacc ttgcgtcgtt tttaccggcg tcgcttttta cccgccgcca atcttcggaa
      301 taagatagat ctcgctaccc ggcttcaact ctgccgcgta ggcatcctgg aaaatctcgc
      361 cgtcgatggc gatcgccatg ctctcctcga cctgtttgcc caggccggga aaccgccggt
      421 ccaactccag aaccagccga cggaaggtgg tcgcctctac ctcgaattcc gtttgaccgc
      481 cggtaaattg ctgcccgacg gaacccgagg agaggacgac cttgggcatc agcccctcaa
      541 tctagagcgc accgcggccc ggtttggacc gcgacgcgct atagcccatc cttttccaga
      601 cagattcacg cctccagacc ttcgaccctc cggcgatctg ctctagccac gcgcgtccag
      661 cgccgcgcga accttcggcg ggctcatcgg cagatccgcc atccgcagtc ctgtcgcact
      721 gcgtatggca ttgccgatcg cggccatagg cggaacaatc ggcacctcgc ctacaccgcg
      781 ggcaccgaac ggatggcgcg gattaggcac ttccaccagc accgcctcga tcatcggtat
      841 gtccgaggcg accggcaccc ggtaatccag gaacccggca ttctccatct gacctttagt
      901 gttgtagatg tattcctcgt tcaaggccca gccgatgcct tgagccgcac cgccctggat
      961 ctgtccctcc acgtagctgg gatggatcgc cttgcccacg tcctggatcg cggtatagcg
     1021 ctcgatcttt acgtgcccgg tttccttgtc aaccgacacg tcacagatat gggcgccaaa
     1081 gccgggaccc gcaccctgcg cattgagcga cacctcggcg atcagcggcc cgcctgtcct
     1141 ggccgccttc aacgccaggg cagccaggtc caacggctcg aaggcgccgg cgttcgagcc
     1201 tgcgggatag gctttgccgt ccttccactc cacagcctcg accgggatgt cccaggtcag
     1261 ggcggcgcgc ttcttcaaat cgtccacgat cttttcagcc gcttgcgtcg ccgccatgcc
     1321 ggtggcgaaa gttacccgcg agccgccggt tacatgggta aagccgatcg acgccgtgtc
     1381 ggcgacaatc gttcgcacgg tcgccgccgg cacgcccagt acctcggcaa ccatcatgcc
     1441 gatggacgcg cgtgagcctc cgatatcggg gctgcccgtc gctgccgtca ccgaaccgtc
     1501 ctcgttcaca tgcacctgtg ccgtcgactc gccaccgatg ttgaaccaga accccgttgc
     1561 caaaccgcgg ccgtggcccg gcttcaacgg cgatttccag tgcggatggg acttcaccgc
     1621 atccagcacc gcggtgaacc cgatgttctg gtgcgtcggc ccgtaatggg ttttcgtccc
     1681 gttcttcgcc gcgttctttt cccgcagcgt cagtgcgtcg atgcccagct tgatggccag
     1741 ttcgtccatc aggctttcca cggcaaacga cgtgatcggc gccccgggtg cccgataggc
     1801 agccaccttc ggccggttgc tgaccacatc gtaaccaacg acatcgacat tcggtaggtc
     1861 atacatggca aaaccgcaca tgcagcccgg cccgatcgga gagccggcga aggcacccgc
     1921 ctgatacttc agcacctgct tggcggcgac gatcgtgccg tccttcttgg cgccgatctt
     1981 gacctccata gccgcacccg aggtcgggcc cgatgcgcgg aacacatcct cccgcgtcat
     2041 ctgcatcttc accgtgcggc cggatttttt cgacagcgcg acggccaccg gctccagata
     2101 gaccagcgtc ttgccaccga agccgccacc gatttccgct gggttgaccc gcaggttggc
     2161 catgttcatg cccagcagct tcgccgtata ggcgcggacc atgaagtggc cctgactgga
     2221 actatggatc gacacctggc cgtccggcga atacgtcgcc agacaggcgt gcggctcaat
     2281 ataggcctga tgcactggct gggtggtata gcggccctcg acgatcacat cggcttcctt
     2341 gaagcccgcc tcggcatcgc ccttccggaa ggtgacaacc ttcgcgatgt tggacggctt
     2401 ggtcggcttc gggtcaacgc cggtggtgaa catatcgtcg tgcaggatcg gcgcgccgtc
     2461 ggccatcgcc tcttcgacat cgatgacgtg cggcaggatc tcgtagttca cgtcgatcag
     2521 cgccagcgcc tcatcggcga tcgcctgggt cgaagcggca acggcggcca gcgcgtggcc
     2581 ttcgtacagc actttcgtcc gcgccatgca attcagcgcc agatcgcgga aattcatcgg
     2641 accttcgccg acgaacgctt cttccgaggg tatgtccggg aaatccgccg cggtgacgac
     2701 agcgaagacg cctttcagtg cctcggcctt cttcgtgtcg atggacacga ttcgagc
//
LOCUS       DASWQN010001123         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_109054, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001123 DASWQN010000000
VERSION     DASWQN010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109054"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /locus_tag="VGC82_19205"
     CDS             <1..1438
                     /locus_tag="VGC82_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEY0425454.1"
                     /translation="ARPDEVHTEIFDLFAALGATDSQLDFPMLYASGRNGWADETLDG
                     PRKDLSPMFDLIVRHVPAPTVKPNAPFAMVASILEYDNFLGRVLTGRVEQGTGRVNMP
                     VKVLRRDGSLVETGRLTKLLSFRGLDRVPVDEVQAGDIIAVAGLSEGTIPDTIGSPEL
                     LGPLEAIPVDPPTLAMTFRINDSPLAGREGKKVTSRQIRERLFREAEGNVAIRVTDSN
                     ETDAFEVAGRGELQLGVLIEQMRREGFELTIGRPRVLTRRNPETNEREEPMEEVLVDV
                     DEPYTGVVVEKLSRRKGDLRDMRPSGGGKVRMTFHIPSRGLIGYHGEFLTDTRGTGIM
                     NRLFAGYGPWKGPIEGRRAGSLISTEGGEAVHYSLWYIQERGTMFVSPGEKVYQGMIL
                     GEHSRESDLEVNPIKEKKLTNIRAAGKDDAMLLIPPRKMSLEQAIAYVEDDEFVEVTP
                     AAIRLRKRYLDPNERKRFERKIDADAAA"
     gene            complement(1549..2874)
                     /locus_tag="VGC82_19210"
     CDS             complement(1549..2874)
                     /locus_tag="VGC82_19210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425455.1"
                     /translation="MTDTSETSDMERTTLRKVYWRLVPFCFLLYILCYVDRINVSFAA
                     LTMNKDLGLSAYVYGFAAGAFFWGYCLLEVPSNIILQKVGARRWIARIMISWGLLSAT
                     TALVTGPTSFFIVRVLIGVAEAGLFPGLLLYFHRWFPQSHRGRVVGWFLTGLPLATAI
                     GAPVSTAFLQMDGIFGLHGWQWMFIGEGLPTALIGVSVLFFLTERPSEARWLAPEQIA
                     WLEDTLRRETHAIEDVRSFGILSAMVNPRLLILTVIFAGIGMAGVGTVLFLPQILKSI
                     GVSNTQAGLLTSIPYVFGTVAIVVCGHLSDKLADRYWTLVVTCGLATIGMILAAMLHD
                     SLWVLAAFGVATIGFYGMKSPFWPLPSTFLTGAALAAGLALINSLGNFAGYLGPILVG
                     YAKDATGTFEAGLYALAAAAFVSTVTALGCALWMPRGAQSAAKLVPASR"
     gene            3131..>4232
                     /gene="argE"
                     /locus_tag="VGC82_19215"
     CDS             3131..>4232
                     /gene="argE"
                     /locus_tag="VGC82_19215"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEY0425456.1"
                     /translation="MTTAELLQRLVSFDTTSRNSNLPLIDFVRGYLDGLGVEYRVSTD
                     PTGEKANVHAIIGPRQPGGLALSGHVDTVPVDGQAWSGDPFLLRQRDGKLFARGSCDM
                     KGFVAACLAAVPDFQARELVRPLHLFISYDEEVGCGGAQRLIQDLDESGLRPDLCVVG
                     EPSGMKPILAHKGKLNLNVSVRGLTGHSSEPSNGVNAVQAAGEAIAWVAREARRLAAE
                     GPFEDGFLPAHTTIHVGTVQGGTSLNIIPEHAEFAMEWRFIPGDSPHRHAERMQAWIA
                     ATIEPAMQAVHPACGFNYDVVLEMPGMALPADHALATAVKQVVGSNSAGKVSYGTEGG
                     FFEAAGIPTIICGPGHIAQAHQPDEWIAESELD"
BASE COUNT          841 a         1322 c         1354 g          715 t
ORIGIN      
        1 cgcccgcccc gacgaggtcc ataccgagat tttcgacctg ttcgctgccc tcggtgcaac
       61 cgatagccag ctcgattttc cgatgctgta tgcctccggg cgcaacggct gggcggacga
      121 gacgctggac ggtccgcgca aggacctgtc gccgatgttc gatctgatcg tccgccacgt
      181 cccggcccca accgtgaagc cgaacgcgcc gttcgccatg gtcgccagca ttctggaata
      241 cgacaacttc ctgggccggg tgctgaccgg gcgcgtcgag caaggcaccg gccgcgtcaa
      301 catgccggtg aaggttttgc gccgcgacgg gtcattggtc gaaaccggcc ggctgacgaa
      361 actgctgtcg ttccgcggcc tggacagggt tccggtcgat gaggtccagg ccggcgacat
      421 catcgccgtg gccgggcttt ccgagggcac catcccggac accattggct cgcccgaatt
      481 gttggggccg ctggaagcca ttccggtcga tccgccgacc ctggcaatga cgttccgcat
      541 caacgactcg ccgctcgctg gccgcgaagg caagaaggta acttcgcgcc agatccgcga
      601 gcggctgttc cgcgaggcgg aaggcaacgt cgccatccgg gtgacggact ccaacgaaac
      661 cgacgcgttc gaggtcgccg gtcgcggcga gcttcagttg ggcgtgttga tcgaacagat
      721 gcgccgcgaa ggcttcgagc tgaccatcgg ccgcccgcgc gtgctgaccc ggcgcaaccc
      781 ggagaccaac gagcgcgagg agccgatgga agaggtgctg gtcgatgtgg acgagcccta
      841 taccggcgtc gtggtggaga agctcagccg ccgcaagggc gacctgcgcg acatgcgtcc
      901 gtcgggcggc ggcaaggtgc ggatgacgtt ccacatcccc agccgcggat tgatcggtta
      961 ccacggcgaa tttctgaccg acacgcgcgg caccggcatc atgaaccggc tgttcgcggg
     1021 ttatggcccg tggaagggcc cgatcgaggg aaggcgcgcc gggtcgctga tctctaccga
     1081 gggcggcgag gccgtgcact actcgctgtg gtacatccag gaacgcggca cgatgttcgt
     1141 gagccccggc gagaaggttt accagggcat gatccttggc gagcattcgc gggaatccga
     1201 cctggaagtg aatccgatca aggaaaagaa gctgaccaac atccgcgcgg ccggcaagga
     1261 tgatgcgatg ctgctgattc cgccgcgcaa aatgagcctg gagcaggcga tcgcctacgt
     1321 cgaggacgac gagttcgtcg aggtgacgcc agcggcgatc cggctgcgca aacgatatct
     1381 ggatccaaac gagcgcaagc gcttcgagcg taagatcgac gccgacgcgg ctgcctgagg
     1441 tctttccggc ccctggggtc tttcctgcca cggcacgacc gccttggccg gaataattct
     1501 cacgcgcatg cgtggcttcg gtatctttga ctaccgcgaa acctttgact accgcgaagc
     1561 cggcactagc ttggccgccg actgggcacc tcgcggcatc caaagggcac aacccaaggc
     1621 cgtcaccgtc gagacgaatg ccgcggcggc cagcgcgtat agaccagcct cgaaggtccc
     1681 cgtcgcgtcc ttcgcatagc cgaccaagat cggccccaga tagccggcga aattaccgag
     1741 cgagttgatc agggccagtc ccgccgcgag cgccgctccg gtcaggaacg tcgatggcaa
     1801 cggccagaac ggggacttca tgccgtaaaa gccgatcgtc gccaccccga acgccgccag
     1861 cacccacagg ctgtcgtgca gcatcgccgc caggatcatg ccgatggtcg ccaggccgca
     1921 ggtgaccacc aaggtccaat agcgatccgc cagtttgtcc gacagatgac cgcacaccac
     1981 gatggcgacc gtgccgaaca cgtatgggat cgatgtcagc agcccggcct gggtgttgga
     2041 cacgccgatc gatttcagga tttgcggcag gaacagcacc gtgccgacgc cggccatgcc
     2101 gatgccggcg aagatgaccg tcaggatcag caggcgcgga ttgaccatcg ccgacaggat
     2161 gccaaagctc cgcacatcct cgatcgcgtg cgtttcgcgc cgcagcgtat cctccagcca
     2221 cgcgatctgc tccggcgcca gccaccgagc ttccgatggg cgttcggtca ggaagaacag
     2281 gaccgagacc ccgatcaggg cggtaggcag cccctcaccg atgaacatcc attgccagcc
     2341 atgcaggcca aaaattccat ccatttgcag gaaagcggtg gacaccggcg caccgatggc
     2401 cgtcgcgagc ggcaaacccg tcaggaacca gccgacgacc ctgccacgat ggctttgcgg
     2461 gaaccagcga tgaaagtaca gcagcaagcc cgggaacaat ccggcttcgg ccacgccgat
     2521 cagtacgcgc acgatgaaga aactggtcgg accggtcacc agtgcggttg tcgctgaaag
     2581 caatccccag gaaatcatga tgcgggcgat ccatcgcctc gcgccgacct tctgcaggat
     2641 gatgttggac ggcacctcca gcaggcagta accccagaaa aaggcaccgg cggcgaagcc
     2701 gtaaacgtag gcgctgagcc ccaaatcctt gttcatcgtc agcgccgcaa aactgacgtt
     2761 gatgcggtcc acatagcaaa ggatatagag caagaaacaa aatggtacca ggcgccaata
     2821 gacttttcgc agcgtggttc gttccatgtc cgaggtctcg gaggtgtccg tcatcgccct
     2881 tcccttccga ccgttcgtct tgtaccccca cggtgtgcta gcccctgggt gggcgcaagc
     2941 gacatcgacg cgatgatcag gcggcgtcca agccatcggc ggtttcatca catagatgtg
     3001 agataggaat attgatgctc ggaccgggat tagctgccga tacggtcttg atccaattgt
     3061 agcagtcggt tgccgcctgc gtgaaccggt atagagtgcc ttcatggtta ccgcccagac
     3121 cgcgacgagg atgacgacgg ccgaattgct gcagcggctg gtgtcgttcg ataccaccag
     3181 ccgaaattcg aatttgccgc tgatcgactt cgtgcgcggg tacctcgacg ggctgggggt
     3241 agagtatcgg gtgagtaccg acccgacagg tgaaaaagcc aatgtgcatg ccattatcgg
     3301 gccgcggcag ccgggtggcc tggcgctgag cgggcatgtc gatacggtcc cggttgatgg
     3361 ccaagcctgg agcggggatc cgtttttact gcggcagcgg gatggcaagc tgttcgcccg
     3421 cggaagctgc gacatgaagg ggttcgtcgc cgcctgcctg gcagcagtgc cggactttca
     3481 agcacgtgag ctggttcggc cgctgcatct cttcatcagc tacgacgagg aggtcggttg
     3541 cggcggcgca cagcggttga tccaggatct ggatgaatcg ggtctgcgtc cggacttatg
     3601 cgtggtcggc gaaccatccg ggatgaagcc gatcctcgcg cacaaaggaa aactgaacct
     3661 caacgtatcc gtgcggggtt taaccggtca ttccagcgag ccatcgaacg gcgtcaacgc
     3721 ggtgcaggcc gccggtgagg ctatcgcgtg ggtcgcccgc gaagcccgtc gcctggccgc
     3781 cgagggacca ttcgaggatg gcttcctccc cgcgcatacc accattcacg tcggcaccgt
     3841 ccagggcggc accagcctga acatcatccc ggaacacgcc gaattcgcaa tggagtggcg
     3901 gtttattccc ggtgactcgc cgcaccgtca cgccgaacgg atgcaggcct ggatcgcggc
     3961 aacgatcgag ccggcgatgc aggcggtgca tccggcttgc ggctttaact acgacgtggt
     4021 cctggaaatg ccgggtatgg cgctgccggc ggatcacgct ctcgccactg cggtaaagca
     4081 ggttgttggc tccaacagtg ccggcaaggt cagttatggg accgagggcg gatttttcga
     4141 agccgccggc atcccgacca tcatctgcgg gccgggacat atcgcgcagg cgcatcagcc
     4201 ggatgagtgg attgcggagt ctgaattgga cg
//
LOCUS       DASWQN010001124         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1692452, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001124 DASWQN010000000
VERSION     DASWQN010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692452"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..749
                     /locus_tag="VGC82_19220"
     CDS             102..749
                     /locus_tag="VGC82_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0425457.1"
                     /translation="MPTLEAIHWGPASKAKPRQLVILCHGLGADAFDLIDLAPSWQHA
                     CPDALFASVHAPFPHDSGFGRQWWSVADRSPPVVEAGIRAAAVYLHGFIDAELARLGL
                     PADAYAIMGFSQGAMMSLFTGLRHTPAPRAILAFSGALVAPETLQTELANRAPVLLVH
                     GEADEAVPVSRSHDAEAALTAAGVPVEAHYIPRLGHGIDDTGISLGALALQRGFA"
     gene            963..1520
                     /locus_tag="VGC82_19225"
     CDS             963..1520
                     /locus_tag="VGC82_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY0425458.1"
                     /translation="MSHIPNLQTLVLNADMQPLSWGPLSVWSWQDALVAVLQERVNPV
                     INYDVEVHSTRQSFPIPSVVALKRFHKRRKVAFTRYHVFLRDRFSCQYCGKTLPAKDL
                     TFDHIVPRSKGGFTSWTNVVTCCQSDNLFKGSRSLKQSGLRLRCAPSEPTPHQIDEAA
                     KWHVARENLHRTWLDFLYWDADLEA"
BASE COUNT          268 a          500 c          472 g          287 t
ORIGIN      
        1 ctgacgccca tggtgtgatg ccgcccacgc tgagtcggat cacccggctc aaagcggtta
       61 ttcgtaagta tcgcatcggc gcataaacgg gagacaccac gatgccaacc ctggaagcga
      121 tccattgggg acccgcctca aaagcaaaac ccaggcaact ggtcattctc tgccacgggc
      181 tcggagccga tgcgttcgac ctgatcgacc tcgcaccgtc ctggcagcac gcatgccccg
      241 acgccctgtt cgcctccgtc catgcgcctt tccctcacga ctccggcttt ggacggcaat
      301 ggtggagcgt ggcggaccgt tcgccgccgg tcgtggaggc gggcatccgc gccgccgcgg
      361 tttacttgca cggcttcatc gacgccgaac tggcacggct tgggcttccc gccgacgcgt
      421 atgcgatcat gggcttcagc cagggcgcga tgatgtcgct gttcaccggg ctgcgtcata
      481 cgccagcccc acgcgcgatc ctggcgtttt ccggcgccct ggtggccccg gaaaccttgc
      541 agacggaact ggccaaccgt gcgcctgttc tcctggtcca tggcgaagca gacgaagccg
      601 tcccggtgtc tcgctcgcat gacgccgagg cggcgcttac cgcagccggc gtgccggtgg
      661 aagcacacta catcccgcgg ctgggccacg gcatcgacga cactggaatt tcccttggcg
      721 cgctcgcgct gcaacgagga ttcgcgtgac gaaacaatga cgtcgcaggg ccccattgca
      781 tagcgggttg cggcgcgatg ggcggtctgt catacatcgc aaaggttttc ggcggcgcac
      841 aagatctggc ggctcgacga tatgccgcca aggcattgcg ctccgaacgg acgggagtcg
      901 cttttgcctg acggcggatc gcagttcctc acgactgttc tgaagacgac ggggcgtggt
      961 ccatgagcca tattcccaac ctccagacgc ttgtgttgaa tgcggatatg cagcccctga
     1021 gctggggtcc gctttcggta tggtcgtggc aagacgccct ggtcgccgtc cttcaggaac
     1081 gggtcaatcc ggtcatcaac tacgacgtgg aagtgcactc gacgcggcag tcgttcccga
     1141 tcccgtcggt cgtggcgctg aaacgttttc ataagcggcg caaggtggcg ttcacccgct
     1201 accacgtgtt cttgcgggac cggttcagtt gccagtactg cggcaagacc ctgcccgcca
     1261 aggacctgac gttcgaccat atcgtgccgc gttcaaaagg cgggttcacc agttggacca
     1321 acgtcgtcac ctgctgccag tcggacaatc tgttcaaggg cagccgctcg ctgaagcagt
     1381 ccggcttgcg gttgcgctgc gccccctcgg agccgacccc gcaccaaatc gacgaggcgg
     1441 cgaaatggca cgtcgctcgg gaaaacctgc accggacatg gctggatttc ctgtactggg
     1501 atgccgacct cgaggcctga tccgcgg
//
LOCUS       DASWQN010001125         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_241697, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001125 DASWQN010000000
VERSION     DASWQN010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241697"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VGC82_19230"
     CDS             complement(<1..787)
                     /locus_tag="VGC82_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEY0425459.1"
                     /translation="MSETKEQTDEELAAAWGAETDEEAPVDSAQATRVLNQSEIDNLL
                     GFDNGPSDGEARTGMQKIISSGLVSYERLPMLEIVFDRLVRIMSTSLRNFTSDNVEVG
                     IDNILSLRFGDYLNSIPLPAMLAVFKAEEWDNQGLMVIDSAMIYSIVDVLLGGRRGTA
                     AMRIEGRPYTTIERTLVERLIQVVLTDLSTSFDPLCPVTFRFERLEVNPRFATISRLS
                     NAAILARLRIDMEDRGGRVELLLPYATLEPVRELLLQQFMGEKF"
     gene            complement(784..1230)
                     /locus_tag="VGC82_19235"
     CDS             complement(784..1230)
                     /locus_tag="VGC82_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEY0425460.1"
                     /translation="MLILIAAPLVVLLLVGSGLWFTGILPHLLGMEKHGEKAGGAEAA
                     RSVPPSYIDIPEMIANLNSANHKPSYVKMAARVEVPKPEDVEKVRAALPRLQDMMQTY
                     LREMRPEELRGSAGTYRLREELLVRANAAVAPAKVSDVLFTQMLVQ"
     gene            complement(1551..>2015)
                     /locus_tag="VGC82_19240"
     CDS             complement(1551..>2015)
                     /locus_tag="VGC82_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY0425461.1"
                     /translation="LDPAAVIAGAHWVVPPAAGARIALAYQGPVAPEAEAAFAVLRDE
                     EPGAGLLAITSSDRLHAGWLDAMRARRGGDRGAVSWVEKVLAPLAPGAAIVSVLDGHP
                     ATHAWLGSVRGQRVVPLGPDRFGQSGDIPDLYREYGLDTDAILDACAQAMLG"
BASE COUNT          368 a          709 c          565 g          373 t
ORIGIN      
        1 cgaatttttc gcccatgaac tgttgcagca gaagttcgcg caccggctcc agcgtcgcat
       61 acggcagcag aagttcaacg cgcccgccgc ggtcctccat gtcgatccgc agcctggcca
      121 ggatggcggc gttggacagc cggctgatcg tggcgaagcg tggattgacc tccagccgct
      181 cgaaccggaa cgtcaccggg cacagcggat cgaagctggt cgacaggtcc gtcagcacca
      241 cctggatcag acgctcgacc agggtgcgct cgattgtcgt gtaaggccga ccttcgatcc
      301 gcatcgctgc cgtgccccgc cggccgccca gcagcacatc gacgatcgag tagatcatcg
      361 ccgaatcgat caccatcagg ccctggttgt cccactcctc cgccttgaac accgccaaca
      421 tcgccggcag gggaatcgag ttcaggtagt caccgaaccg cagcgacaaa atattgtcga
      481 tcccgacctc aacgttgtcc gaggtaaagt tgcgcagact ggtcgacatg atccgcacca
      541 ggcggtcgaa cacgatctcc agcatcggca ggcgctcgta ggacacgagg ccggagctga
      601 tgatcttctg catgcccgtg cgggcttcgc catccgatgg gccattgtcg aagcccagca
      661 ggttgtcgat ttcggattgg ttcaggaccc gcgtggcctg tgcactgtcg accggcgctt
      721 cttcgtcagt ctccgcgccc caggcagcgg caagttcctc gtcggtttgt tcctttgtct
      781 cgctcattgg accaacattt gcgtaaacag cacgtcactg accttcgctg gggcgaccgc
      841 cgcattggcg cgaaccagca gttcttcccg cagccggtag gttccggcgg aaccgcgcag
      901 ctcttcggga cgcatctcgc gcagataggt ctgcatcatg tcctgcagcc ggggcagggc
      961 agcacgaacc ttctcgacat cctcgggctt cggcacctcg accctggcgg ccatcttcac
     1021 gtaggacggc ttgtggttcg cactgttcag gttggcaatc atctccggaa tgtcgatgta
     1081 gctcggaggc actgatctgg ccgcctcagc gccgcctgcc ttttcgccat gcttttccat
     1141 ccccagcagg tgcgggagga tgccggtgaa ccacaacccg gaaccgacca gcagcaacac
     1201 cacaaggggc gcggcgatca gaatcagcat acgcttgccg ccctttttgg ccggcggtgc
     1261 ctcaatctcg tccgttttgg cgggggcggt gaactcatgc cgccgagggt gacagataac
     1321 ggttaacgaa aggttgacgc cggcgctgtc ggcccggttc ggcactgaat ccccttaatg
     1381 atgaccatgg tagggtcgtt tgatccgttc aatcagacgg atcaaccgta tgcagaggat
     1441 cccagcgtca cccagccgct gacctgaaac tgcggattta gtgttcgcgc ccagaccacg
     1501 tgccgagacc gatctccctg gcgtcgctcg gttggacaat cggccgtctt ctaccccaac
     1561 atcgcttgcg cacacgcatc caggatcgca tccgtatcca acccatactc ccgatataga
     1621 tccgggatat ccccgctctg cccgaatcgg tctggtccca gtggcaccac ccgctgtccc
     1681 cgcacggatc ccaaccaggc gtgcgtcgcc ggatgcccat ccagtacgct cacaatcgct
     1741 gctcccggtg ccagcggcgc cagcaccttc tccacccacg acaccgcccc gcggtcacca
     1801 ccacgacggg cgcgcatcgc gtccagccac cccgcgtgca gccggtccga ggacgtaatc
     1861 gccagcaatc ccgcccccgg ttcttcgtca cgcagcaccg caaacgctgc ctcagcctct
     1921 ggtgccaccg gcccctgata tgccagcgct atccgcgccc cagccgctgg cggcaccacc
     1981 caatgcgccc ccgcaatcac cgccgctggg tccag
//
LOCUS       DASWQN010001126         3284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2221507, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001126 DASWQN010000000
VERSION     DASWQN010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221507"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1063)
                     /locus_tag="VGC82_19245"
     CDS             complement(110..1063)
                     /locus_tag="VGC82_19245"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0425462.1"
                     /translation="MNTFKLVDPELRDALALWPRLPLTAESLTQRRADLLRVRGAVPP
                     PDLPDITAGEIHVESAFGATPIRVLTYRPVKSDNPLPTIVHIHGGGFVMGAPEMKDVE
                     NRLLASELGCAIYSVDHRLAPEAPHPAPLEDIYSVFAWLHANARQLGLDPARIGIKGE
                     SGGGGFAAGVALYARDQKGPQFAFQHLIYPMIDDRTAVRKDLHPQVGEFVWTQGNNYF
                     GWRSLLGREPGGAGVSPYAAAARAADVSGLPPTYISVGGLDLFLEENMVYADRLSRAG
                     VPVEFHMYPRTYHGFYQATDARVTRQAERDTREALRRFLHG"
     gene            complement(1227..2474)
                     /locus_tag="VGC82_19250"
     CDS             complement(1227..2474)
                     /locus_tag="VGC82_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0425463.1"
                     /translation="MFGSVPRRGLMMGGLATGGLAAIASPRVAWSQSKPIRIGVLTDM
                     VGVYAANTGLGSVNGAKLAIEDFAKLQPNVKVELVSADLQLKPDVAVGIAGDWLDNQG
                     VDLITDVPLSSAAFAIGDMVKAKDKVAIFTGGASAAITGERCGPNHLHWVYDTWSMPH
                     AVVDATVKEGGDTWYFITADYAFGRALESDAASFVAAANGKVLGQALAPFPGTTDFSS
                     HLVQARASGAKVIGFANGGGDTVNCIKQSAEFGIQKGGQKIAGLLFLIHDVRGVGLAS
                     AQGVLVTEPFYWNLNAGTRDFAARFGKLMPGNVPGSVHAGQYSAVMHYLKACVALGPE
                     KAKASGRAVIERMKATPTDDPLFGKGAVRKDGRVIHDMYLFEVKSPDQSKEPWDYYTH
                     KRTVPAAEAFRPIDKGGCALALG"
     gene            complement(2574..3035)
                     /locus_tag="VGC82_19255"
     CDS             complement(2574..3035)
                     /locus_tag="VGC82_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0425464.1"
                     /translation="MSKSKLKIKSVSGPRRQYAALPVQLAGDGTPRVLLLTSRGTRRW
                     VIPKGWPMNGMSPAAAAAREAYEEAGLEGTIEDAVPIGHYHYEKGLPGGRSVRLQVAV
                     FLLIVSRQLPTWPEQAERETRWCDPEEAAELVAEPELAAILRALRPARMAG"
BASE COUNT          624 a         1101 c          974 g          585 t
ORIGIN      
        1 cggcaagcca gatagttcac ccacacgtga taaccgcgct tatcgcgtgt gggtggccaa
       61 aacacaggtg cttccagcca cgaactgtgt cgcctgacag cgctctggat taaccgtgca
      121 ggaagcgccg cagagcctcg cgcgtgtcac gctcggcctg tctggtcacg cgggcatccg
      181 tggcctgata aaagccatga tacgtccgcg gatacatgtg aaactcgacc ggcacccctg
      241 cccggctcaa ccgatccgcg taaaccatgt tttcttcgag gaaaagatcg agaccgccga
      301 cagaaatgta ggtcggaggc aagcccgaca catccgccgc ccgcgcggct gccgcgtaag
      361 gcgagacgcc ggcgccacct ggctcccggc cgagcagcga acgccagccg aaatagttgt
      421 tcccctgcgt ccacacgaat tcgccgactt gcggatgcaa gtctttgcgc accgccgtgc
      481 ggtcatcgat catcgggtag atcaggtgct gaaaggcgaa ttggggcccc ttctggtcgc
      541 gggcataaag tgcgacgcct gccgcgaaac cgccgccacc gctttcgccc ttgatgccga
      601 tgcgggcggg atcaagcccg agctgtcggg cgttggcgtg cagccaggcg aacaccgaat
      661 agatatcctc cagcggcgcc gggtgcggtg cctccggcgc caggcgatga tcaaccgaat
      721 aaatggcgca tccaagttcc gacgcgagca gcctgttctc cacatccttc atctcgggcg
      781 cgcccatcac gaagccgccg ccatggatgt gtacgatggt gggaagtggg ttgtcggatt
      841 tgaccgggcg gtaggtcaga acgcggatcg gcgttgcgcc aaacgcgctc tcgacatgga
      901 tttcgccagc ggtgatatcg ggaagatccg gcggcgggac ggcgcctctg accctgagca
      961 gatcggcgcg gcgctgcgtc agcgactccg cggtcagcgg cagccgcggc cacagcgcga
     1021 gcgcatcgcg cagttcggga tcaacaagtt taaatgtgtt catggtgacg gctgcgtccg
     1081 cttgatgccg gacaaaacac tagggtggca cttgccgggc cacaagctgc agggctgcat
     1141 caccaggcgc caggcggttc ccacggtgca gacccaaaga gcatgatcgc ccttggcgga
     1201 acggttgaag ctgtcaatca tgcgccctac cccaatgcga gggcgcaccc acccttgtcg
     1261 atcggtcgga acgcctcggc cgccgggacc gtgcgtttgt gggtgtagta atcccacggc
     1321 tccttcgact ggtcgggcga cttcacctcg aacaggtaca tgtcgtggat gacccttccg
     1381 tcctttcgca ccgcgccttt gccgaacaac gggtcgtcgg tcggggtcgc cttcatccgc
     1441 tcgataaccg cccggccgct tgccttggcc ttttccggtc cgagtgccac acatgctttc
     1501 aggtaatgca tcactgccga atactggccg gcgtgcaccg atcccggaac gttgccgggc
     1561 atcagcttgc cgaaacgtgc tgcaaaatcc cgcgttcccg cgttcaggtt ccagtagaac
     1621 ggttcggtta ccaacacgcc ctgggcggat gccaggccga cgccccggac atcatggatc
     1681 aagaacaaca ggccagcgat tttctggccg cctttctgga tgccaaactc agccgactgc
     1741 ttgatgcagt tcaccgtatc gccgccgccg ttagcaaagc cgatgacctt ggcgccgctc
     1801 gcccgcgcct gcaccagatg ggacgaaaaa tcggtcgtgc cgggaaacgg cgccagcgcc
     1861 tgaccaagca ccttgccgtt ggccgcggca acaaaactgg ccgcatcgct ttccagcgct
     1921 cggccgaaag cgtagtcggc ggtaatgaag taccaggtgt cgccaccctc tttcaccgtc
     1981 gcatcgacca cggcatgcgg catcgaccag gtgtcataga cccagtgcag atggttcggg
     2041 ccgcatcgct ccccggtaat ggccgccgac gcaccgccgg tgaagatcgc caccttgtcc
     2101 ttggctttca ccatgtcgcc gatggcgaac gcggctgacg acaagggcac atcggtgatc
     2161 agatcgacac cttgattatc gagccagtca ccggcgatgc caaccgcgac atcaggcttc
     2221 aactgaaggt cggcggagac aagctcgacc ttcacattcg gctggagctt ggcgaagtcc
     2281 tcgatggcaa gcttcgcccc gttcaccgaa ccgaggccgg tgttggcggc ataaacgccc
     2341 accatgtcgg tgagaacccc gatacgaatc ggcttcgact gcgaccaggc gacgcgcggc
     2401 gacgcaatcg ccgccagccc acccgtcgca agtccgccca tcatcaggcc gcgccgtgga
     2461 actgatccaa acatgtttcc tccccgctcg atttttcgcg gggagcgtct cccggttcag
     2521 cttatttcgc aagccgtcca ggctgacgcg ctatagcggc gcttacaaag cggttaccct
     2581 gccatcctcg ccgggcgaag cgcgcgtaga atggccgcaa gctccggctc cgccaccagt
     2641 tcggccgcct cttcagggtc gcaccagcgc gtctcccgct ccgcctgctc gggccatgtc
     2701 ggcaactgac gcgacacaat caacaggaaa accgccacct gcagccggac agaccggccg
     2761 cccgggaggc ctttttcata gtggtaatgc ccgatcggca ccgcgtcctc gatcgtcccc
     2821 tccagcccgg cttcttcgta cgcttctcgc gccgccgccg ccgcaggtga catcccgttc
     2881 atcggccagc ctttcgggat aacccagcgg cgggtaccac gcgaggtaag caggagaacc
     2941 cgcggtgttc cgtcgccggc caactgcacc ggaagcgctg catattgccg ccgcggaccg
     3001 gaaactgact ttatcttcag cttggacttc gacacgatcg atccaccggg aatgttgccg
     3061 agcgattcaa acgccgacca gcgcccgtga cgatgctgcc tgaggcatcg ggccaaaatc
     3121 aactaccaac gccgtcctga agcgcctcct ttccttgcca gtgctggatc ggccagtgca
     3181 ggatcggcca ttactggatc ggccaacact gggtcggcca gcgctagatc ggccagtgct
     3241 ggatcggcca gtgctggatc gaagtcgttc tcgcctcagg cggt
//
LOCUS       DASWQN010001127         4679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1429841, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001127 DASWQN010000000
VERSION     DASWQN010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429841"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="VGC82_19260"
     CDS             <1..1464
                     /locus_tag="VGC82_19260"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY0425465.1"
                     /translation="VQKVDLTPDLKRVQVTIRMNREAEPLLTDNAKFWVVRPRFFAGS
                     ISGLQTLFSGSYIDLLPAAGSGEQKRDFVGLENPPVLQTDVPGRTFLLQASRIGSLNL
                     GSPIMFRDLEVGEVLGWDVGEMARDITVHAFVREPFIQYVHDNSRFWNASGAKVQLGG
                     NGLQLQIESLRAVVLGGIAFDTPEDSRFATASQSNHTFPLFQSEDAAVSSAFKRSVAF
                     LANFTGSAAGLAAGSAVTVHGLKIGEVSSVSLEFDRGLDNVIVPVHFSLEPERIAMLD
                     LPKDGDLDIRMRDLVRRGLRVKLETANFLTGQKQLAMDIYRDAGSAEVTKQGDAYVIP
                     VLGGSSDDVATAATNLVNRLSDIPFDAIGKNLDQTLAGVNALVNDKQLGDSLAALRST
                     LASVQTLVTNLNHGLAPTMQRLPTIATGLEDTVKRTEKLVGSLESGYGDDSRFSRDVS
                     RMMMQLSDAARSLRVLADLLSRHPEALIRGRTDQGVQ"
     gene            1461..2042
                     /locus_tag="VGC82_19265"
     CDS             1461..2042
                     /locus_tag="VGC82_19265"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HEY0425466.1"
                     /translation="MTGYRRLALLVRRFGLLLIMAFPAACSSPNPSLYVLAAVPGATQ
                     GGGPRIVELRAISIPHYLERSQIVRSSEGYRMDVLSNEWWGEPLDTMMSRVLVQEISQ
                     RLPRSTVYSDNGAISTPPDATVAINLQRLDLDRDGAVLLAAQIAVERKTSAAKGVTFT
                     VRPADATTQALVAAMSTATGQLADAIAVLLAGR"
     gene            complement(2161..3012)
                     /locus_tag="VGC82_19270"
     CDS             complement(2161..3012)
                     /locus_tag="VGC82_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0425467.1"
                     /translation="MIEGGRWKRWVYFYSPMSLFIVVLLFPFYWMVITSIRPDGELYR
                     PWNARNYNPFWTWNPTLEHIQTLLQETLFATWLWNTMLIAVASTAISLVCGVFAGYAL
                     SRLKFRFAATLGTGIFITYLVPQTLLFIPLADIIRNFHLGDTPWSLILTYPTFLIPFS
                     TWLMMGYFKAIPKELEECARIDGASRWQAMIYIILPVAVPGILSAGIFAFTLSWNEFI
                     YALVFLSSSEQKTVPVGVVSELVRGDVFYWGSLMAGALLGSVPVAVVYSFFVEHYVAG
                     LTGSVKG"
     gene            complement(3050..3979)
                     /locus_tag="VGC82_19275"
     CDS             complement(3050..3979)
                     /locus_tag="VGC82_19275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY0425468.1"
                     /translation="MASASLTMLVAARPRRKAEGLEDERWFAAALLFPTAVLLGLFIA
                     YPFFEGIWLALTDTRVGVPGHFVGLKNFEKVWNDAIFRTAVWNTCWYTFVTTIFKLVL
                     GLWLAQLLNRHFRGKAWVRAFILLPFIIPTVLSTFAWKWMFDPTFSVLNWLLWHVGLI
                     KGRINWLGEPDLAFASIIVVNIWRGVPFYAISLLAGLQTISPELQEAAAIDGANAWQR
                     FVHITWPLLLPVTMVVTLFSVIQTFADFQLVYVLTGGGPANATHLFATYAYQIGVGTG
                     LLSEGAAISLAIFPVLFFVVLVQLIYIRRVETR"
     gene            complement(4149..>4679)
                     /locus_tag="VGC82_19280"
     CDS             complement(4149..>4679)
                     /locus_tag="VGC82_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425469.1"
                     /translation="LSGTVAATLNGASIYIESLRKPDQYQTEKGRPLKEDILHATLPA
                     GPKGQFSNHLFHSHVIPNYSKNQAQAKALLKWFHTPANYEKWFVSFKGFNTAPTVVWE
                     GHKMWDEDPVMTPFRAAAAAGRVPGFAGQSNQKAAEVLNKYIVVDMYAKAVQGMPAEQ
                     AAAWAEGELKKVYAAG"
BASE COUNT          940 a         1441 c         1431 g          867 t
ORIGIN      
        1 gtacaaaaag tcgatcttac gcccgacctg aagcgggtgc aggtgaccat tcggatgaac
       61 cgcgaagcgg aaccgctgtt gaccgacaac gcgaagttct gggtggtccg tccgcgcttt
      121 ttcgccggct cgatcagcgg cctgcagacg ctgttctccg gctcctatat cgatctgctg
      181 ccggccgcgg gaagcggcga gcaaaagcgc gattttgtcg ggctggaaaa tccccccgtg
      241 ctgcaaaccg atgttccggg ccgcaccttc ctgttgcagg caagccgcat cgggtcgctc
      301 aatctcggat ccccgattat gttccgtgac ctggaagtcg gagaagtcct tggctgggat
      361 gtgggcgaga tggcccgcga catcaccgtg cacgccttcg tgcgtgagcc gttcattcag
      421 tatgtgcacg acaattcgcg gttctggaac gcgtccgggg cgaaggtgca gcttggcggc
      481 aacgggttgc agttgcagat cgagtcgctc cgtgccgtgg tgctcggcgg catcgccttc
      541 gatacgcccg aagattcaag gttcgccacc gcgagccaat ccaatcacac attcccgctg
      601 ttccagtccg aagacgccgc cgtgtcctcg gcgttcaaac gcagcgtagc gtttctggcg
      661 aacttcaccg gatcggctgc cgggctggcg gccggttccg cggtcaccgt gcatggcctg
      721 aaaatcggcg aggtttcaag cgtatcgctg gagttcgacc gtgggctgga taacgtcatc
      781 gtgcccgttc atttcagcct ggagccggag cgtatcgcga tgctggacct gcctaaggac
      841 ggcgacctgg acataaggat gcgggatctg gttcggcgtg gcctgcgggt gaagctggaa
      901 accgccaact tcctgaccgg ccagaagcag ttggcgatgg acatctatcg ggatgcgggc
      961 agcgcggaag tgaccaaaca gggcgacgcc tatgtcattc ccgtcctggg cggcagcagc
     1021 gacgatgtcg ctaccgcggc taccaatctg gtcaaccggc tgagcgacat accgttcgac
     1081 gcgatcggca agaacctcga tcaaaccctg gcaggcgtaa acgcactggt gaacgacaag
     1141 cagctcggtg actcgctggc ggcacttcga tcgaccttgg ccagcgtgca gacactggtg
     1201 accaacctca atcacggcct cgcgccgacg atgcagcgac taccgactat cgctaccggg
     1261 ctggaggaca cggtcaagcg cactgaaaag ctggtcggat cgctcgagag cgggtacggc
     1321 gacgattccc gcttcagccg cgatgtcagc cggatgatga tgcaattatc cgacgcggca
     1381 cgttcgttgc gcgtgctggc ggacttgttg tcgcgccacc cggaggcact gatccgcggc
     1441 cgtaccgacc agggggttca atgacaggtt accggcgcct cgcgttgttg gtccggcgct
     1501 tcgggctgtt gctgatcatg gcttttccgg cggcttgttc gtcgcctaat ccctcgttgt
     1561 atgtcctggc ggcagtgcca ggcgcgaccc agggcggcgg gcccagaatc gtcgaactgc
     1621 gagcaatctc catcccgcat tacctggaac gctcgcagat cgtccggtcg tccgaaggct
     1681 atcgcatgga tgtgctgtcg aacgaatggt ggggcgagcc gctcgacacg atgatgagcc
     1741 gcgtgctggt gcaggaaatc agccagcggc tgccgcgcag cactgtgtat tctgataatg
     1801 gtgccatctc cacgccgcct gacgcgaccg tggccatcaa cctgcaacgg ctggacctgg
     1861 atcgcgacgg ggcggtgctg ctggccgcac agattgccgt ggaacgcaaa accagcgccg
     1921 cgaagggggt gacgttcacc gtgcggccgg cggatgcgac cacccaggcg ctcgtggcgg
     1981 caatgagtac ggcaaccgga caactcgccg acgcgatagc agtcctgctg gccggacgct
     2041 gactgccgcg gcggcaatgg acggttggcg atctatgtcc agccggctgc gagcacggcg
     2101 ccgccgagac accacgcctg acgagtcccg ccggcgggta tggtcgtcat gccgggtaga
     2161 tcatcccttc accgaccccg tcaggcccgc cacgtaatgt tctacgaaga acgagtaaac
     2221 caccgccacc ggcaccgaac ccaagagtgc gcctgccatc aacgaacccc agtagaacac
     2281 gtctccccgc accagttcgg agacaacacc caccggaacc gtcttttgtt ccgaggacga
     2341 caggaagacc agcgcgtaga tgaattcgtt ccatgacaac gtgaaggcga aaatacccgc
     2401 cgacaggatt cctggcacgg ccaccggcag aatgatatag atcatcgcct gccagcgcga
     2461 cgcaccgtcg atgcgggcgc actcctccag ttcctttgga atcgccttga agtaccccat
     2521 catcagccag gtgctgaacg ggatcaggaa tgtcgggtag gtcaggatca gcgaccacgg
     2581 cgtatcgccg agatggaaat tacggatgat gtctgccaat ggaatgaaca acagggtctg
     2641 cggcacgaga taggtgatga agataccggt ccccagtgtg gccgcgaagc gaaacttcag
     2701 ccgcgaaagc gcgtagcctg cgaacacgcc gcacaccaag gagatcgccg tggaggccac
     2761 tgcgatcagc atcgtgttcc acagccaggt ggcgaacagc gtctcttgca gcagtgtctg
     2821 gatatgctcg agagttgggt tccaggtcca gaacggattg tagttcctgg cgttccaggg
     2881 gcggtacagt tcaccgtcgg gccgtatcga ggtgatcacc atccagtaga atggaaataa
     2941 caacacaacg ataaacagcg acattggcga gtagaaatag acccagcgct tccagcgtcc
     3001 gccctcgatc atcagcttgc ccctgtctcg ttgttttcgc gcggtcttct cagcgcgtct
     3061 cgacgcgacg aatgtaaatc aactgcacca ggaccacgaa aaacagcacc ggaaagatcg
     3121 ccaaggaaat cgccgcgccc tcggaaagca atccggtccc gacgccgatc tgataagcgt
     3181 aggtagcaaa cagatgcgtg gcattggccg gaccgccgcc ggtcagcaca tagacaagtt
     3241 gaaaatcggc aaaggtctgg atgactgaaa acagcgtcac caccatcgtc accggcagca
     3301 gcagcggcca agtgatatgc acgaagcgct gccaggcgtt ggcaccatcg atcgccgcgg
     3361 cttcctgtag ttcggggctg atggtttgca gcccggccag caggctgatc gcgtagaacg
     3421 gcaccccgcg ccagatgttc acgacgatta tcgaggcaaa ggcaagatcc ggctcgccca
     3481 gccaattgat ccggcccttg atcaggccga cgtgccaaag cagccaattc agcacgctga
     3541 aggtcggatc gaacatccat ttccaggcga acgtggacag cacggttgga atgatgaacg
     3601 gcagcaggat gaaggcacgc acccaggcct tgcccctgaa gtgccggttc agcaattgcg
     3661 ccagccaaag tcccaacacc agcttgaata ttgtcgtgac gaacgtgtac cagcaggtgt
     3721 tccacaccgc cgtgcgaaag atcgcatcgt tccagacttt ctcgaagttc ttcagcccga
     3781 cgaaatggcc gggcacgccg acgcgggtat cggtcagggc cagccaaatg ccttcgaaaa
     3841 acggataggc gatgaacagg cccagcagga cggccgtggg aaacaacaac gcggcggcga
     3901 accaccgttc gtcctccagc ccctctgctt tgcggcgggg ccgagcggcc acgagcatcg
     3961 ttaacgaagc gcttgccact tctcgccctc ccctttggtt cgtcccaact tgattgtgcc
     4021 gggcttgact tgtgccaact tgattgtgct gggcttgatt tgtgtccggc ccggatgcgt
     4081 cctcgttcgc ttcaaggggt cgcttccgga caggccttat tgattcgccc gcaggccttc
     4141 gttccgggtc agccggcggc ataaaccttc ttcagttcac cctcggccca tgccgccgcc
     4201 tgctccgccg gcatgccctg caccgccttc gcatacatat cgacgacgat atacttgttc
     4261 agcacttccg cggctttctg attggactgg ccggcgaacc ccggcacgcg acctgccgcg
     4321 gcggcggcgc ggaagggagt catgaccggg tcctcgtccc acatcttgtg gccttcccac
     4381 accaccgtcg gcgcggtgtt gaaccccttg aagctgacga accacttctc gtagttcgcc
     4441 ggggtgtgga accacttcag cagcgcctta gcctgcgcct ggttcttcga gtagttcggg
     4501 atgacgtggc tgtggaagag gtggttcgag aactgcccct tcgggccggc cgggagggtc
     4561 gcgtgcagga tgtcctcctt gagcggacgg cccttctcgg tctggtactg gtcgggcttt
     4621 cgcagcgact cgatgtaaat ggacgcgccg ttgagcgttg ccgcgacggt gccggacag
//
LOCUS       DASWQN010001128         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_110126, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001128 DASWQN010000000
VERSION     DASWQN010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110126"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VGC82_19285"
     CDS             <1..709
                     /locus_tag="VGC82_19285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEY0425470.1"
                     /translation="RQHEMPFAGHPNVGTGYLLATMAGDPPEHYTFEEMAGLVRVHVL
                     RDANRAISGARVAAPRSLSIDIGIPVEVVAACAGLKEADIATLSHTPLVASVGTPFVI
                     AEVASVEALSRASPDLAAFRAALARFPEMDGRFALHLYAWMQGDERRIRCRMFAPLSG
                     TSEDPATGSAAATLAALLTSMAAGDNVDLHYAIEQGVEIGRLSLIIASAVKTGEGPVS
                     ASVAGSCVPVMRGTLQV"
     gene            complement(710..1168)
                     /locus_tag="VGC82_19290"
     CDS             complement(710..1168)
                     /locus_tag="VGC82_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0425471.1"
                     /translation="MSDELFTLDTNVLVYSVDRRAGHRHEIAKDIVRRASLRQCHLTL
                     QSISEFYAAVTRKRMMPLAGAAQFARDMIETFPTAAASASAVRFALTSAANGHASYWD
                     ALLVATAAEAGCTTILTEDLADGTLLCGVRVLNPFGDNNLSAAAAALFAE"
     gene            complement(1158..1406)
                     /locus_tag="VGC82_19295"
     CDS             complement(1158..1406)
                     /locus_tag="VGC82_19295"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY0425472.1"
                     /translation="MTRTLSLRDANQTFARCVREAEAGEELIITRNGTPVARLSPIGK
                     RRVLTPEQQAAWQRIDAAMEQGWDIGAGPLDRDALHER"
     gene            complement(1479..>1678)
                     /locus_tag="VGC82_19300"
     CDS             complement(1479..>1678)
                     /locus_tag="VGC82_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HEY0425473.1"
                     /translation="TMFAPIQDRDATGQGFTHKYGDIVTVEAPKLGRLVNRMMRTDQC
                     EAWNFGSGALMRNLAGRGLLK"
BASE COUNT          284 a          529 c          542 g          323 t
ORIGIN      
        1 ccgccaacac gagatgccgt tcgctggaca tccaaacgtc ggcaccggct accttctggc
       61 gacgatggct ggcgatcccc ctgagcatta cacgttcgag gagatggcgg gcctggttcg
      121 cgtgcacgtt ctgcgcgatg cgaaccgggc gatttccggc gcccgcgtgg ccgccccgcg
      181 gtcgctgagc atcgatatcg gcattccggt ggaagtcgtc gcggcctgtg ccggcttgaa
      241 agaagccgac atcgcgacct tgtcgcacac gcccttggtc gcctcggtcg gcacgccgtt
      301 cgtgatcgcc gaggtcgcga gcgtcgaggc attgtcccgc gccagcccgg atcttgccgc
      361 cttccgggca gctttggccc gatttcccga gatggacggg cgattcgcgc tccatcttta
      421 tgcctggatg cagggcgacg agcggcgcat ccgatgccgc atgttcgcac cattaagcgg
      481 tacgtccgag gatccggcga ccggcagcgc ggctgccacc ttggcggcgc tgctgacctc
      541 gatggcggcg ggcgacaatg tggatttgca ctatgcgatc gagcagggcg tggagatcgg
      601 ccgactgagc ctgattatcg cttctgcggt gaagacgggc gaggggccgg tgtcggcgag
      661 cgtggcggga agctgcgtcc cggtgatgcg gggaacgctg caggtctgat cattcggcga
      721 acagtgccgc ggcagccgcg ctcaggttgt tgtcgccaaa cggattaagc accctgacgc
      781 cgcagagcaa cgtgccgtca gcgagatctt ccgtcaggat ggtggtgcac cccgcctcgg
      841 cggcggtggc gaccaaaagc gcgtcccagt atgaggcatg accgttggct gcgctggtga
      901 gtgcaaagcg gaccgcgctg gccgaagccg cggcggtggg gaaagtctcg atcatgtccc
      961 tggcgaactg cgcggcgccg gccaatggca tcatgcgttt gcgtgtgacc gcggcataga
     1021 actcggagat cgattgcaga gtcaggtggc actgccgaag gcttgctcgg cggacgatat
     1081 cctttgcgat ctcgtgccgg tggcccgctc gccgatccac gctgtaaacc agaacattgg
     1141 tgtccaacgt gaataattca tcgctcatgc agcgcgtcgc gatcgagcgg acctgctccg
     1201 atatcccatc cttgctccat tgcggcgtcg attcgttgcc acgccgcctg ctgttcgggc
     1261 gtcagcacgc gccgtttgcc aatgggcgac aggcgcgcaa ccggcgtgcc attacgggta
     1321 atgatcaatt cctcgccggc ctctgcctcc cgaacgcagc gggcaaaggt ttgattggcg
     1381 tcgcgaaggg acagggtcct ggtcatccac acgaagtagc acgggttgtc ctacatttca
     1441 acccacgttg cgttttaaca gttcacgtac cggcgatgct acttcagcaa tccccttcca
     1501 gccagattgc gcatcagcgc cccggaccca aagttccacg cctcgcactg atcggtgcgc
     1561 atcatccggt tcaccaggcg gccaagcttc ggcgcctcga cagtcacgat atcgccgtac
     1621 ttatgggtga aaccctgacc tgttgcgtcg cggtcctgga tcggcgcgaa catcgtgc
//
LOCUS       DASWQN010001129         5856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1033359, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001129 DASWQN010000000
VERSION     DASWQN010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033359"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..470)
                     /locus_tag="VGC82_19305"
     CDS             complement(177..470)
                     /locus_tag="VGC82_19305"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0425474.1"
                     /translation="MTKFGDALIESLGDALGHARGEKSDVRLHTVELLDVRAIRRKLH
                     MSQQAFAATYRIPLPTLKNWEQGRRHPDAPASAYLLAISRLPAEVGAALAEEP"
     gene            complement(474..644)
                     /locus_tag="VGC82_19310"
     CDS             complement(474..644)
                     /locus_tag="VGC82_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425475.1"
                     /translation="MGKRGGVRVIYFYHDDSMPLYLLLIYAKAERESWTRDEKRRAYT
                     LTENIKQAYRRH"
     gene            complement(657..806)
                     /locus_tag="VGC82_19315"
     CDS             complement(657..806)
                     /locus_tag="VGC82_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425476.1"
                     /translation="MTVAETGPFIRQAIRLWTDEDRHAFVDFIAANPNTGDIIPDTGG
                     LRKIR"
     gene            complement(866..2041)
                     /locus_tag="VGC82_19320"
     CDS             complement(866..2041)
                     /locus_tag="VGC82_19320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0425477.1"
                     /translation="MNILVWQWGRRGAGPRFAACLAESLRERPGIEVILSLCREAEIM
                     AGPNPPRCEMPVWTYRGMVSLLLRLITAPAALPFLIGRLRRVRPDLAICAMPGPLDLL
                     MAIALRLLGARIVVVVHDADVHPGDGFPGQMLLQRWLCRHAGGLAVLCGHVASRLRQQ
                     RLAGSPKRPLIPFKHPPFAFDMPARDQRPGKPRLLCFGRLLEYKGLDLLAEAMATLPP
                     NAEIELRVVGSGPETAALDALRACRGVTVENRWVPEAEIGSLLAWSDALILPYREGSQ
                     SGVAAAALAAGRSVIATRVGGLKEQLSQAPQAVLCEPDAGSLARAISLWLEAPELIFE
                     PIDATASWRQAASDLLQAIATALPAKLRQSDSRLPGWDVSHARRSSSTSTIAAHRRH"
     gene            complement(2038..3045)
                     /locus_tag="VGC82_19325"
     CDS             complement(2038..3045)
                     /locus_tag="VGC82_19325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0425478.1"
                     /translation="MADAARRLGLPVWIIDLPSPVGARAKVEATKCLSPPPGVPLVLH
                     VNAPLLPLALLRLPSAVTSNRSIIGYWAWEMPEIPPEWRPAIACVSQIWVPSRFTAAA
                     LEPLLPGRVRVVPPPLGLVPPVASACDRAAFGLPSDAVVVLVSFNLASSFVRKNPFAA
                     VAAFRSAFGDRPDRVLMLKVSHPEHAPADFARLTQMARAPNIHIDTRTLPSDDRHALT
                     ACADIVLSLHRGEGFGLVMAEAMLLGKAVIATGWSGNTDFMDDTNAALVGYRLAPARD
                     DRSVYRGLWAEPDVAEAASLLRTLADDAASRRSLGERARHSTMARLDGRPLLAALADL
                     G"
     gene            3423..4652
                     /locus_tag="VGC82_19330"
     CDS             3423..4652
                     /locus_tag="VGC82_19330"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY0425479.1"
                     /translation="MAVKNVKKVVLAYSGGLDTSVILRWLQTTYGCEVVTFTADLGQG
                     EELEPARKKAEMAGVREIFVDDLRETFVRDFVFPMFRANTVYEGQYLLGTSIARPLIA
                     QRQIEIAEQVGADAVAHGATGTGNDQVRFELSYYALKPDIKIIAPWREWELTSRTRLL
                     EFAEKHQIPIAKDKRGEAPFSVDANLLHSSSEGKVLEDPAVEADEAVYQRTIRPEDAP
                     DQATVIAMDFVSGDPVAIDGVALSPAGVLTRLNELGKANGIGRLDLVENRFVGMKSRG
                     VYETPGGTILLAAHRAIESITLDREAAHLKDSLMPRYAELIYNGFWFSPERRMLQALI
                     DTSQHSVSGRVRLKLYKGNVTCIGRESPNSLYSMEVVTFEDDQGAYDQIDAQGFIKLN
                     ALRLRLGAMAGRRGGTL"
     gene            4964..>5856
                     /locus_tag="VGC82_19335"
     CDS             4964..>5856
                     /locus_tag="VGC82_19335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0425480.1"
                     /translation="MMGTMDQVDASFSEDDRHRLLTETITDYAIYILDTSGLVTSWNR
                     GAQRLTGNDEREVLGKHFSIFFTDQDQRDGLPNRALECSARDGTFQIECWQVSKDGRR
                     IWAHTVLDSIRNEKNELEGFAQITRDLTERKRADEFLKRNDDQFKLLVQNVADYAIYM
                     LDVEGMISSWNTGAQQIKGYLADEIIGRHFSLFYTDEDREKGAPAKSLEVAAQEGKFA
                     QDGWRVRKNGAVFWASVVISPIRDDDGSIIGFAKVTRDLTHAKKAQRDLERARDTLFQ
                     SQKMEAIGQLTGGVAHDFNNIL"
BASE COUNT         1192 a         1741 c         1897 g         1026 t
ORIGIN      
        1 cgagccaatg gcgggtttcg tcaatcaggg tttcaccgac atcgaagcag atggcacgaa
       61 tgggcattga tggtcgcatg atccttccag gggtgccggc gccccaggcc gatgagacgc
      121 ctgtcgcgcc ggctgcaggg atcatgttgt ccgctgctcg cgctggcaat gccaggtcac
      181 ggctcttccg ccagggccgc gccgacttcg gccggaagcc gcgaaatcgc cagcagatag
      241 gcgctggccg gggcatccgg gtggcgccgg ccctgttccc agtttttcaa cgtcggcagg
      301 gggattcgat aggtcgccgc gaacgcttgc tgcgacatgt gcagcttgcg gcggattgcc
      361 cgcacgtcca gcaattcaac cgtatgcagc cgcacgtccg atttttcgcc acgcgcatgg
      421 ccgagcgcat cgcccaggct ttcgatcagc gcatcgccaa acttggtcat tgttcaatgc
      481 ctccgatagg cctgcttgat gttctcggtc agtgtatagg cgcgtcgctt ttcgtcgcgg
      541 gtccagcttt cccgttccgc tttggcgtaa atcagcagca ggtacagcgg catcgagtcg
      601 tcgtggtaga aatagatgac ccttactccg ccccgtttgc ccattcccgg ccgggatcaa
      661 cgaatctttc gtagcccgcc ggtgtccgga atgatgtccc cggtgttggg attcgcggca
      721 atgaagtcaa caaaggcatg gcggtcttcg tcggtccaaa gtctgatggc ctgacggatg
      781 aacggccccg tttccgcgac cgtcatcaat ccaccttcta ttgacccatg cgccattggc
      841 gcctccggtg caagagaaaa aggcgtcagt ggcgtctatg ggcggcaatg gtactggtcg
      901 aggatgatcg tcgtgcatgc gaaacatccc aaccaggaag tcgggaatcg gactggcgca
      961 gcttcgctgg cagcgcggtg gcaattgcct gcagcaggtc tgacgccgcc tgtcgccagg
     1021 acgccgtagc gtcgattggc tcgaagatta gttccggcgc ttccagccaa aggctgattg
     1081 cacgagccag gctgccggca tccggctcac acagcacagc ctgtggcgcc tgggacagtt
     1141 gttccttcag tccgccgacc cgcgtggcga taaccgagcg tccggcggca agtgccgctg
     1201 ctgcaacgcc gctttggctg ccttcgcgat agggcaggat cagggcatcg gaccaggcca
     1261 gcaagctgcc gatctcagct tccggaaccc agcgattttc caccgtgacg ccgcggcacg
     1321 ccctcagtgc atccagcgcc gctgtttccg gtccgctgcc gacgacccgc agctctattt
     1381 cggcgttcgg cggaagcgtc gccatggcct ctgccagcag gtccaatccc ttgtattcca
     1441 acaggcggcc aaagcagagg aggcgcggct tgccaggcct ctggtcccgg gcgggcatgt
     1501 cgaaggcgaa gggcgggtgt ttgaagggaa taagcggccg cttcggcgat ccagccaggc
     1561 gttgctgccg cagccgggat gcgacatgtc cacacagcac cgccagcccg ccggcgtggc
     1621 ggcataacca gcgttgcaac agcatctggc ccggaaaacc atcgccagga tgcacatccg
     1681 catcatgcac cacgacgaca atgcgtgccc caagcagccg cagtgcaatc gccatcagca
     1741 gatccagcgg ccccggcatg gcgcagatcg ccaggtccgg cctgacccgg cgaagcctgc
     1801 cgatcaggaa cggcagagcg gccggtgccg tgatcaggcg cagcaacagc gacaccatgc
     1861 cgcggtatgt ccacactggc atctcgcagc gaggcggatt tgggcccgcc atgatttcgg
     1921 cctcccggca gagcgacagg atcacctcga taccgggccg ctcgcgcagc gactcggcga
     1981 ggcaggcggc gaaacgtgga ccggcaccgc ggcggcccca ttgccagaca aggatattca
     2041 tccaaggtcc gccagcgccg cgagcaacgg ccggccgtcg agcctggcca tcgtgctgtg
     2101 gcgagcccgc tcaccgagcg atcttcggct ggctgcatcg tcggccagtg tccgcaagag
     2161 cgaagctgcc tcggctacgt ccggttccgc ccaaagtccg cgatacaccg aacggtcgtc
     2221 acgcgccggt gccagccggt agccgaccag ggcagcattc gtgtcgtcca tgaagtcggt
     2281 gttgccggac cagccggtag caatgacggc tttacccaag agcattgcct cggccatcac
     2341 cagcccgaag ccttcgccgc gatgcagtga caggacgata tcggcgcagg cggtcagggc
     2401 atggcggtcg tccgaaggca gggttcgcgt atcgatatgg atatttggtg cccgcgccat
     2461 ttgcgtcagg cgagcaaagt cggccggcgc atgttcggga tggctgactt tcagcatcag
     2521 cacgcgatcc ggccgatcgc cgaacgcgct gcgaaaggct gcgacggccg caaacggatt
     2581 cttgcgaacg aatgaagatg ccaggttgaa ggacaccagc acgacgacag cgtcgctggg
     2641 cagtccgaac gccgctcggt cgcacgccga ggcgactggc ggaaccaagc ctaaaggcgg
     2701 aggaacgact ctgacccgcc cgggcagcaa cggctccagc gccgccgcgg tgaagcggga
     2761 tggtacccaa atctggctga cgcaagctat cgccgggcgc cattccgggg ggatttcggg
     2821 catttcccag gcccagtagc cgataatgga tcgattactg gtgacggcgg acggcaggcg
     2881 cagcaacgcc agcggcagca ggggcgcgtt gacatgcagg accagcggca cgccgggcgg
     2941 cgggctcagg catttggttg cctcgacttt ggcccgggca cccaccgggg agggtagatc
     3001 gatgatccag accggcaagc cgagccgcct ggccgcgtcg gccatgagcc gggcaccttc
     3061 gcccagaccg gaggcgcgag tgaactcccc ggctatggca attcctgcgg cgcagggacc
     3121 agggggcggt gaaggtctgg gggcgatcca actggtcgca cgtgccagcg cccgccggcg
     3181 caggaccggg ggcaaaccgc gccaaaaggc atgggacgcg gtcggaacaa atcgcccgga
     3241 tccaggcggg gtgagggaag gctgcatgcc cggatgatcg gggacaaccc ttaacaaccg
     3301 tttaaagtgg catcgaattc cgccgcctgt ccggttgctg tccgtggccg gcgacccaat
     3361 gacggcttgc cggtatggac ggcgggcgct acttcggcct cagaagccgg agaatccttg
     3421 ccatggctgt gaagaatgta aaaaaggtcg tgctcgccta ttccggcggg ttggacacca
     3481 gcgtgatcct gcgctggctg cagacgacct atggctgtga agtcgtaacc tttaccgccg
     3541 atcttgggca gggcgaggaa ctcgagccgg cgcgcaagaa ggccgagatg gcgggcgtca
     3601 gggagatctt cgtcgatgac ctgcgcgaaa ccttcgttcg cgacttcgtc ttcccgatgt
     3661 tccgcgccaa cacggtttat gagggccagt atctgcttgg cacctcgatc gctcggccgt
     3721 tgatcgcgca gcgtcagatc gagatcgccg aacaggttgg agccgatgcc gtggcgcacg
     3781 gcgcgaccgg gacgggcaac gaccaggtgc gtttcgagct gtcgtattat gcgctgaagc
     3841 cggacatcaa gatcatcgcg ccatggcgag agtgggagtt gacgagccgc accaggctcc
     3901 tggagtttgc cgagaagcat cagatcccga tcgccaagga taagcgcgga gaggcgccgt
     3961 tcagcgtcga tgcgaacctg ctgcactcct cgtccgaagg aaaagtcctg gaagacccgg
     4021 cagtggaagc ggatgaggcc gtttatcagc ggaccattcg gcctgaggat gcgccggacc
     4081 aggccacggt catcgccatg gattttgtta gcggcgatcc ggtggcgatc gatggcgtgg
     4141 cgctgtcgcc cgccggcgtc ctgacgcggt tgaacgaact cgggaaggcc aacggcatcg
     4201 ggcggctgga cctggtggag aaccgtttcg ttgggatgaa gtcccggggt gtttatgaaa
     4261 cgcctggagg gacgatcctg ctggcggcgc accgggcgat cgaaagcatc acgctggatc
     4321 gcgaggcggc acacctgaag gacagcctga tgccgcgata cgcggagctg atctacaacg
     4381 gcttctggtt cagcccggag cggcggatgc tgcaggcgct gatcgacacc agccagcaca
     4441 gcgtttcggg ccgggtgcgg ctgaaacttt acaagggcaa cgtcacctgc attgggcgcg
     4501 agagcccgaa cagcctgtat tcaatggagg tcgtgacgtt cgaggacgac caaggcgcct
     4561 atgaccagat cgacgctcaa gggtttatca aattgaacgc attgcggctg cggctgggcg
     4621 cgatggcggg gcgtcggggc gggacgcttt agagacagag gtaaagtcag cagaattcct
     4681 ccagtcccag cttagctgca ttcccttcga cgttcgccgg gccgtcggca agcggcctgt
     4741 gagatcacat gagacaaaga tgccatgtaa ctgatccgga aggacgtcgg ccgggatgtc
     4801 tggttcgagg cgttcgaaca atgcccgtag ataaaccaat caataatcaa atgcatcttg
     4861 gatcacaata atgtgaatac agaagggaaa tgttggcaac catcaagaga tgatgctgtg
     4921 ttctagtagt ttcgaaggtg gtaaccaacg tagccggaga gacatgatgg gaacgatgga
     4981 tcaagtagac gcatcgttct cggaagacga ccggcaccga ctccttacag agacgattac
     5041 tgattacgcg atttatatcc ttgacacctc gggccttgtc acaagctgga accgcggtgc
     5101 gcagcggctt acgggaaatg acgaacggga agttctcggc aagcacttct cgatcttctt
     5161 cacggatcag gatcagcgcg atgggcttcc gaaccgagca ctcgagtgtt cggcgcgcga
     5221 cggaacattc cagattgagt gctggcaagt aagcaaggat ggacgacgca tctgggctca
     5281 taccgtcctt gactcgattc gtaatgaaaa gaacgagctt gagggattcg cgcagatcac
     5341 gcgcgacctg accgagcgca aacgcgccga tgaattcctc aagaggaatg acgatcagtt
     5401 caagcttctg gttcagaacg tagccgacta cgcgatctac atgctcgacg tggaaggaat
     5461 gatcagcagt tggaacaccg gggcccaaca gatcaagggc tatcttgcgg acgagattat
     5521 aggccgccac ttctcgctgt tttatactga cgaagaccgg gagaaaggcg cgcccgcgaa
     5581 gtccctggaa gtcgctgctc aagaaggtaa gttcgcgcaa gacggatggc gcgttcgaaa
     5641 aaatggcgcg gtcttctggg cgagtgtagt gatcagcccg atacgggacg acgatggcag
     5701 cattatcggg tttgccaaag tcacgcgcga cctgacccac gccaaaaagg cgcagcggga
     5761 cctggagcgt gctcgcgaca cgctttttca gtcgcagaag atggaggcga tcggccagct
     5821 gacaggcggt gtcgcgcatg acttcaacaa catcct
//
LOCUS       DASWQN010001130         3186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_110253, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001130 DASWQN010000000
VERSION     DASWQN010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110253"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VGC82_19340"
     CDS             complement(<1..796)
                     /locus_tag="VGC82_19340"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0425481.1"
                     /translation="MPAAVSLRVAAVAFTLVFGMGRAGAAEPPITIGFGMALTGGIAA
                     NGRAALIAMKLWEEDINKAGGLLGRPVKLIYYDDQSTPPNVPGLYTKLLDVDKVDIVV
                     SGYGTNMTVPAMPIVMAHNRMFMTLFALAVNADFKYPRFFGMIPAGPAADAKRAYSRG
                     YFETAKAMNPRPKTLAIVGADAEASRNAVEGALDNAKEAGLEVVYNRSYPPTTNDFSP
                     IVRAIQAAHPDVVYIASYPADSVGFVNALHEVGMKARLVGGSMVGLQ"
     gene            complement(862..2067)
                     /locus_tag="VGC82_19345"
     CDS             complement(862..2067)
                     /locus_tag="VGC82_19345"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0425482.1"
                     /translation="MNDIAGASLTASLDDAGVAAIARARTLIPLLRSAADRIDEKNEL
                     PADVLDAMFDAGMFKLLLPRAIGGLELKPTDFIQCVEAIAEGDASAAWCMNQGSGCSM
                     AAAYLSPEVASEIYGGKRDVVAWGQGPGAKATRADGGWRVTGTWSFASGSRHATWLGA
                     MCPSFGADGTAIMRPDGKAWERTFMFRRDKAKIDDVWQVMGLRGTGSDNYTIKDLFVD
                     DAHSLTREYDPERRQHGALYSFQAMQLYASGFASVALGTSRAMLDAFITVAKTKSQAW
                     SSDSLRDNHAVQHIVGYSDAAWKAARAGLHKAVDDAWRDVSLTGVLSLDHRIQIRQAA
                     TYAIHTSRDMAHHVFHEAGSTAIFNKHPFERRLRDLNSVSQQLQGRRTHLESVGHYML
                     GGEPSLRWI"
     gene            2212..2697
                     /locus_tag="VGC82_19350"
     CDS             2212..2697
                     /locus_tag="VGC82_19350"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0425483.1"
                     /translation="MKLMWFQLMLYTELSADFRDAKPSVWVDIRSTRFDPKRAHLMHN
                     GFMDELEYAAEVGFDAICVTEHHSSGYGSMLSPNLIASSLARRTSDRAICVMGNSLAL
                     YNPPTRVAEEFAMINGIPGGRLTAGFLVGTPMDTCNAYGTNPSQLCERYHQAYDLIMA
                     T"
     gene            2694..2903
                     /locus_tag="VGC82_19355"
     CDS             2694..2903
                     /locus_tag="VGC82_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425484.1"
                     /translation="MSKNLTRYDTRLFAEHVMPKLRDVHAGWTDHWWPQAMEPAQRAN
                     VPVYRPNLFAKSSLTLTINDTRSAN"
     gene            2900..>3186
                     /locus_tag="VGC82_19360"
     CDS             2900..>3186
                     /locus_tag="VGC82_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425485.1"
                     /translation="MSQPETLTIDVNGFSTRIWRQGTGPKLGFLAGFGGLPRWMPFLD
                     ELAKSSTVIVPSLPGFPGGDRGHSVLDSHLDWLLAVREILDQAGLAGANLAG"
BASE COUNT          599 a         1028 c          951 g          608 t
ORIGIN      
        1 tctgcaaacc gaccatactg ccgccgacca gccgcgcctt catccctacc tcatgcaggg
       61 cattgacgaa gccgacgctg tcggccgggt aggaggcgat gtaaaccacg tccggatgag
      121 cggcctgaat cgctcggacg ataggtgaaa agtcgttggt ggtcggcggg taggaccggt
      181 tatagaccac ttccagcccg gcctctttgg cattgtccag cgcgccttcg acggcgttcc
      241 gtgatgcctc cgcatccgca cccacgatcg ccagcgtctt cggccgcgga ttcatcgcct
      301 tcgcggtttc gaagtagccc cttgagtatg cccgcttggc gtcggcggcc gggccggctg
      361 gaatcatccc aaaaaaccgc gggtacttga agtcggcgtt aaccgccaag gcgaacaacg
      421 tcatgaacat gcgattgtgg gccatgacga tgggcatcgc cggcaccgtc atgttggtcc
      481 cgtagccgga cacaacgatg tccactttgt ccacgtccag cagcttcgtg tacagccccg
      541 ggacattcgg cggcgtgctc tgatcatcgt aatagatcag cttgaccggc cgcccgagca
      601 ggccgccggc cttattgata tcttcttccc acaatttcat ggcgatcagc gccgctcggc
      661 cgttggccgc gatcccgcct gtcagtgcca tgccaaaccc gatggtgatc ggcggttcgg
      721 cagcgccggc tcgccccatc ccgaagacca acgtaaaagc gaccgcggcc acgcggagcg
      781 aaacagcagc tggcatcgac aaccctccct tcgcggccgc tcgtttcgcg accgcggagc
      841 gacgctatcg cctccgatag gttatatcca gcgtaggctg ggctcgcctc cgagcatgta
      901 atgccccacc gactcgagat gtgtccggcg cccctgcaat tgctgggata cgctgttgag
      961 gtcgcgcagc cgccgctcga atgggtgttt gttgaagatc gcggtcgagc cggcctcatg
     1021 gaacacgtgg tgcgccatgt cgcgtgatgt gtggatcgcg taggtcgcgg cctgccggat
     1081 ctgtatccgg tggtccagcg acagcacgcc tgtcagcgac acatcgcgcc acgcgtcgtc
     1141 aacggccttg tgaaggccag cgcgcgctgc tttccacgcc gcatcggagt agccgacgat
     1201 gtgctgaacc gcgtgattat cccgcaatga atcagaggac caagcctggc ttttggtctt
     1261 cgccacggtg atgaaggcat ccagcatggc ccgcgatgta cccaacgcga cactggcgaa
     1321 accgctggca tacaactgca tcgcctggaa cgagtacagc gcgccgtgct gccgccgctc
     1381 ggggtcgtat tcccgcgtca ggctgtgcgc atcgtcaacg aacaggtcct tgatggtgta
     1441 gttgtcgctg cccgtgccgc gcaggcccat cacctgccag acgtcgtcga tctttgcctt
     1501 gtcgcgccgg aacatgaagg tccgctccca ggccttgcca tccgggcgca tgatcgccgt
     1561 cccatcggcc ccgaagctcg ggcacatggc accaagccag gtcgcgtgcc gcgagccgct
     1621 ggcgaaactc caggtgccgg tgacacgcca gccgccgtcg gcgcgggttg ccttggcccc
     1681 cggaccctga ccccaggcga ccacatcgcg cttgccgccg tagatctcgc ttgcgacctc
     1741 gggggataga taggcagcgg ccatcgagca cccggaccct tgattcatgc accacgccgc
     1801 ggacgcatcg ccctcagcga ttgcttcgac gcattggata aaatcggtcg gcttcagctc
     1861 aaggccacca atggcccggg gcagcaggag tttgaacatg ccggcgtcga acatcgcgtc
     1921 cagcacatcc gccggcagtt cgttcttctc gtcgatccga tccgctgcag atcgcagcaa
     1981 cgggatcagg gtgcgcgccc gtgcgatcgc ggccacgccg gcatcgtcga gagaagccgt
     2041 tagggatgcg ccggcaatgt cattcatgcg accagatcct caattgccag accctctgtt
     2101 ctacgaagat tgcccgaagc ccaaggccgg ctccacgaag gtcgccgtga aattgacatg
     2161 ccaacaaggc cctcgcaata ctcgtcggta accaggaacc ggaggtcgtt catgaagctg
     2221 atgtggttcc aattgatgct ctacacggaa ctgtcagcgg atttccggga cgccaaaccg
     2281 tcggtatggg tggatattcg ttccacgcgg ttcgatccga agcgcgccca tctcatgcat
     2341 aacggcttca tggacgagct tgaatatgcc gcagaggtcg gcttcgacgc catttgcgtg
     2401 accgagcatc actccagcgg ctacggatcg atgctgtcgc ccaacctgat cgcatcgtcg
     2461 ctggcacgcc ggacctccga tagggcgatc tgcgtgatgg gcaactcact agcgctctac
     2521 aatcctccga cgcgggtggc ggaggaattc gccatgatca acggcatccc cggcggtcgg
     2581 ctgactgccg ggttcctggt cggcacgccg atggatacct gcaacgccta tggaaccaat
     2641 cccagccagc tttgtgagcg ctatcatcag gcgtacgacc tgattatggc aacatgagca
     2701 aaaacttgac acggtacgat accaggcttt tcgccgaaca tgtcatgccg aagctgaggg
     2761 acgtacatgc aggctggact gatcattggt ggccgcaggc gatggagcct gctcagcgcg
     2821 ccaatgttcc ggtttaccgg cccaacctgt tcgccaaatc gagcctgacg ctgaccatca
     2881 acgacacgcg gagcgcaaat tgagccaacc tgaaacgttg accatcgacg taaacggctt
     2941 ttccacccga atctggcggc aaggcactgg cccgaaatta gggttccttg ccggctttgg
     3001 cggcttgccg cgctggatgc cgttcctgga tgaactggcg aaatccagca ccgtcatcgt
     3061 gccatcgctg ccggggtttc ccggtggcga ccggggtcat tcggtactgg actcccacct
     3121 ggactggctg cttgcggtgc gggaaattct cgaccaagcg ggtctggccg gggcgaattt
     3181 ggccgg
//
LOCUS       DASWQN010001131         3941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_505515, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001131 DASWQN010000000
VERSION     DASWQN010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505515"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VGC82_19365"
     CDS             complement(<1..455)
                     /locus_tag="VGC82_19365"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY0425486.1"
                     /translation="MQHRRTGVYSREQLSRLLHPASIAVVGASTRAGSFGERVIFNMK
                     HYAGRSFPVNARYQTIGDQTCYPNIADLPEVPDCAVITAPREAVEEIVLDCARAGVGG
                     AIIFASGYAETGKEDRAVQQERLAAIARETGLRIVGPNCIGVVNALLDSR"
     gene            571..1356
                     /locus_tag="VGC82_19370"
     CDS             571..1356
                     /locus_tag="VGC82_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0425487.1"
                     /translation="MSSLFDLTGKVAIVTGSTKGIGLAIATRMAEHGATVVISSRKPD
                     SCEAVAAGIREKGGKAVVIPCHIARKEELQNLVDQTVAQCGGIDILVENAGVNPYLGP
                     AAGMPDEAYERVMGSNVRSNLWLANMAIPHMVQRGGGSLIIISSIGGLRGSAQLGAYA
                     ISKAADMQLARNMAVEWGPKNIRANAIAPGLVRTDFARALWEDPALYRKRTRDTPLQR
                     IGEPDEIAGAAVFLASAAGSFTTGQTIVIDGGTTAGSVISHDD"
     gene            complement(1366..2391)
                     /locus_tag="VGC82_19375"
     CDS             complement(1366..2391)
                     /locus_tag="VGC82_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0425488.1"
                     /translation="MPQQPHARFAGAIDTDWLARHVEPILEPDLPIIDPHHHLWMRDG
                     NTYLLPELLADLGSGHNIRATVFEECHAMYRADGPPEDASLGETEFVTGIAAMGASGN
                     FGPTRVCARMVGGVDLRLGEAAFPLLERHIQASGNRFAGIRQSTAWDASDRIHKVVPQ
                     PRMLADPAFRTGYAALGRLGLVFDAWVYHPQLHEVADLAGAFPDTTIVLNHVGSPILG
                     GPYAADRDQVFADWRAGMAALARHPNVTVKLGALPIRLPGSTASRELPPSSQEVADVW
                     RPWLETSIELFGADRCMFESNFPVQKRWSSYAVTWNAFKRIAAAASVAEKAALFAGTA
                     ARVYRVP"
     gene            complement(2473..2862)
                     /locus_tag="VGC82_19380"
     CDS             complement(2473..2862)
                     /locus_tag="VGC82_19380"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0425489.1"
                     /translation="MTLLADACALIVFHGYGGQTMSKAGKTAMSTGDVFVSPITVWEI
                     SRKVALGKLERPAPPGFNGTLSEWLRQSGYRALPLTWDACERANGLPMHHKDPMDRML
                     IATALDRGLKVVTDDEIFANYGVTTIW"
     gene            complement(2859..3254)
                     /locus_tag="VGC82_19385"
     CDS             complement(2859..3254)
                     /locus_tag="VGC82_19385"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY0425490.1"
                     /translation="MDDAGRQAEVGWVEQWFHIDLMRSRQIIPVLPAGKDGRYGLTGQ
                     IAVTIYLVMNKAITLAEAKAHLSALVTEAEEGAEITITRHGRPVARVVGAPRQATRTA
                     GDWGWRGTYDKSVFAPMTDEQMREEGWPA"
     gene            3201..>3941
                     /locus_tag="VGC82_19390"
     CDS             3201..>3941
                     /locus_tag="VGC82_19390"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0425491.1"
                     /translation="MKPLFDPADFRLAPGVVHVCAGGETAALRSHDQALLRYVADKSS
                     GLPGRTAQEAEIESARARLARLWGVPAADIGFVSNVAEGVSIVAESLDWRDGDSIAID
                     ANEYPSVVGPIAMRRKPSVALRQARGMQPDRLAACVDGSTRIIATSYVSYLTGERVDL
                     HALRRLADNVGALLLVDFTQASGYLPIEASIADFAFSACYKWMLGITGVGVAYWNRTR
                     QPNWMPSSAGWHSLAEGSRGYDTRPALKS"
BASE COUNT          683 a         1275 c         1292 g          691 t
ORIGIN      
        1 cggctgtcga gcagcgcgtt caccacgccg atgcagttcg gaccgacgat tcgcagcccg
       61 gtctcccgcg cgatcgcggc caggcgctcc tgctggacgg cgcgatcttc ctttcccgtc
      121 tcggcatagc cggatgcaaa aataattgcg ccaccgacgc cggcgcgggc gcagtcgagg
      181 acgatttcct ccacggcctc acgcggtgcc gtgatcaccg cacagtccgg aacctccggc
      241 aggtcggcga tgttcggata gcaggtttgg tccccgatcg tctgataccg cgcgttcacc
      301 ggaaaggatc ggccggcata gtgcttcatg ttgaaaatca cgcgctcgcc gaacgacccg
      361 gctcgggtgg aggcgccgac cacggcgatg ctcgccggat ggagcagccg gctgagttgt
      421 tcgcggctgt aaaccccggt gcgcctgtgt tgcattgatc ctgcctgatt gacgttccct
      481 gagcggccag catactcacc ggtagcgtcg cggcaaccca ttcgcggcag cctgcgcggc
      541 taaaccaacg agtccaaggg aaaaacgaag atgtcgtcac tgttcgacct cacgggaaaa
      601 gtcgccatcg tgaccggttc aaccaagggc atcgggctgg cgatcgccac aagaatggcg
      661 gagcacggcg cgacagtggt gatatccagc cgcaagcccg actcgtgcga ggcagtcgcc
      721 gcgggcatcc gcgagaaagg cggcaaagcg gtcgtcatcc cttgccacat cgcccgtaag
      781 gaggaattgc agaacctggt ggaccagacg gtggcgcagt gcggcgggat cgacatcctg
      841 gtcgagaatg ccggcgtgaa cccctatctc ggtccggctg cgggaatgcc ggatgaggcc
      901 tacgaacgtg tcatgggttc gaatgtgcgc agcaacctgt ggctggcgaa catggcgatc
      961 ccgcacatgg tgcagcgcgg cggcggatcg ctgatcatca tttcctcgat cggcggactg
     1021 cgcgggtcgg cgcagttggg tgcctacgcg atttccaagg cggccgacat gcagttggcg
     1081 cgcaacatgg cggtcgaatg ggggccgaag aacatccggg ccaacgcgat cgcccccgga
     1141 ctggtgagaa cggatttcgc ccgggcgctt tgggaggacc cggcgctgta tcgcaagcgc
     1201 accagggaca cgccgctaca gcgcatcggc gagccggacg agatcgctgg agcagcggtg
     1261 ttcctcgctt cggccgcggg cagtttcacc accggccaga cgatcgtgat cgacggcggg
     1321 acgacggcag gatccgtcat ttcccacgac gactgaggca cgcgctcacg gcacccggta
     1381 aacccgtgcc gcggtgcccg caaacagtgc ggccttctca gccaccgacg ctgcggctgc
     1441 gatccgtttg aacgcattcc aggtcaccgc gtagctgctc cagcgtttct gcaccggaaa
     1501 gttgctctca aacatgcagc ggtcggcgcc gaacagctcg atgctggttt cgagccaggg
     1561 gcgccagacg tcggcgacct cctgcgagga cggcggcagt tcacgcgatg cggtgctgcc
     1621 gggcaggcgg atcggcagcg caccgagctt gacggtgacg ttggggtggc gggcaagggc
     1681 ggccatgccg gctctccagt cggcgaacac ctgatcgcgg tcggcggcat agggtccgcc
     1741 caggatcgga ctgccgacgt ggttcaacac gatggtggtg tccgggaagg cgccggcgag
     1801 atcggcgact tcgtgcagtt gcggatgata cacccaggca tcgaacacca ggcccagccg
     1861 cccgagggcc gcgtagccgg ttcggaaggc cggatcagcc agcatgcgcg gttgcggcac
     1921 caccttgtgg atacgatcgc tcgcgtccca ggcggtggac tgccggatgc cggcgaagcg
     1981 gttgccgctg gcctggatgt ggcgttccag cagagggaag gccgcttcgc ccagccgtag
     2041 gtccacgcca ccgaccatgc gggcgcagac acgcgtcggg ccgaagttgc cgctggcgcc
     2101 catcgcggcg atgccggtga cgaattcggt ctcgccgagg gatgcatcct caggcgggcc
     2161 atctgcacgg tacatcgcgt ggcattcctc gaacaccgtg gcgcggatgt tgtggccgga
     2221 gccgagatcc gccagcagtt ccggcagcag ataggtgttc ccatcgcgca tccacaggtg
     2281 gtggtgcggg tcgatgatcg gcaggtcggg ttccaggatg ggttcgacgt gccgggccag
     2341 ccagtccgtg tcgatggctc cggcgaagcg ggcgtgtggc tgctgtggca tggacgtttc
     2401 cccgtttttg tcgcggcggc acggcgcgag acctgacgat ggcatcgggt gggccatcgt
     2461 gacagcccac cttcaccaga tcgtggtcac cccatagttc gcgaagatct cgtcgtcggt
     2521 gaccaccttt aggccccggt caagagcggt ggcgatcagc atccggtcca tcgggtcttt
     2581 gtggtgcatg ggcagcccgt tcgcccgttc gcacgcgtcc caggtcagcg gcagggcgcg
     2641 atatccagac tgacgcagcc actccgataa cgtgccgttg aagcccggcg gagccggacg
     2701 ctccagcttg cccagcgcga ccttgcgaga gatctcccac acagtgatgg gggatacgaa
     2761 gacgtccccc gtgctcatgg ccgtcttccc ggccttgctc atggtctggc cgccatagcc
     2821 atggaagacg ataagggcgc aagcgtccgc cagcagggtc aagccggcca gccttcctca
     2881 cgcatctgct cgtcggtcat gggcgcgaag acagacttgt cgtaggttcc gcgccacccc
     2941 cagtcgccag cggtgcgagt cgcttgccgc ggcgccccga ccacgcgagc gaccgggcgg
     3001 ccgtggcggg tgatcgtgat ttccgcacct tcctcggcct cggtgaccag ggccgacaag
     3061 tgagcctttg cttcggcaag agtgatagct ttgttcatga ccaaataaat ggtcacagca
     3121 atctggcctg tcaagccata tctcccatcc ttgccagccg gcagcacggg aataatctgc
     3181 cgcgatcgca tgaggtcaat atgaaaccat tgttcgaccc agccgacttc cgcttggcgc
     3241 ccggcgtcgt ccatgtctgt gccggcggcg agactgccgc tcttcgctcc cacgatcagg
     3301 cactactgcg atacgtcgca gataaaagtt cggggttgcc cggtcgcacc gcgcaggagg
     3361 cggaaattga atccgctcgc gctcgcctgg cgcgtctgtg gggcgtgccc gccgccgata
     3421 tcggcttcgt cagcaacgtc gccgagggcg tctccatcgt cgccgaaagc ctggactggc
     3481 gggacggcga ctccatcgcc atcgatgcca acgaatatcc ctccgtcgtg ggtcccatcg
     3541 ccatgcgacg caagccgtcg gttgcgttac gccaggcgcg gggcatgcaa cccgatcggc
     3601 tagccgcgtg cgtcgatggc agcacccgca tcatcgccac atcctatgtc tcctatctca
     3661 ccggtgagcg cgtcgatctt cacgcccttc gacgcctggc ggacaatgtc ggcgcattgc
     3721 tgctggtcga tttcacccaa gcgtcgggct atctgccgat cgaggcctcc attgccgact
     3781 tcgccttctc cgcctgctat aaatggatgc tcggtattac cggcgtcggc gtcgcctact
     3841 ggaatcgaac cagacaaccg aactggatgc cttcatccgc cgggtggcac tccttggcag
     3901 agggttcgcg cggctacgat acgcgcccgg cattgaaatc c
//
LOCUS       DASWQN010001132         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2089930, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001132 DASWQN010000000
VERSION     DASWQN010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089930"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VGC82_19395"
     CDS             <1..473
                     /locus_tag="VGC82_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425492.1"
                     /translation="LGAADAHLAAEAVRGLERAKWRLWHGRRPGCRRKLAALCRWARR
                     PSIYGMAGIGRLEHHVSELPAYLERNRGALVHYAARHQNGEPISTAFVESAVNEIIAK
                     RMNKKQQMRWNRATVQPFLDVRTAVLNDTLEDAFRHRYPGFHPANGNEAVALAG"
     gene            complement(817..1890)
                     /locus_tag="VGC82_19400"
     CDS             complement(817..1890)
                     /locus_tag="VGC82_19400"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY0425493.1"
                     /translation="METPFWEGGPEPIRGTTYPLTDDALLDWYATSMGMRAVRVMICW
                     EAIQDNPNPLPAPPVAAGAMGAIPVATGGRQIYWTDLVQLVIRLIDRGIYVAVCPWQH
                     NPASSNTDITYRGLGFEPEHFADFWGKFATAINASIAAGFPPDPRFPADAARPDRLAF
                     DLINEPHEAVGGADVGISFAGWQACAQQAINAIRADAGNQNTILVEGMGFASARLDRS
                     QADLGDPESALWKGLADPLGNLAISVHCYEGTRILPGEAAAEKPADAMRKACDDLVGW
                     ARGHGLKVHIGEVAVDAGVGGCSSLPQAQDKWADWNDFCRDNDDVLMGWNWWANTSKN
                     WGWPDEGSCQVGKPDIAGGRNWR"
BASE COUNT          375 a          684 c          697 g          397 t
ORIGIN      
        1 gccttggcgc ggccgacgca cacctggccg cggaggcagt tcgcggactg gagcgtgcga
       61 agtggcgctt gtggcatggg cgtcggccag gatgccggcg caaactcgcg gcgctatgcc
      121 gctgggcgcg gcgtccatct atatacggta tggctggcat cgggcgccta gaacaccacg
      181 tcagcgaact tccggcctac ctcgagcgga accgaggagc gctggtccac tacgccgcca
      241 ggcaccagaa tggcgaaccg atctcgacgg cgttcgtgga gagtgcggtc aacgagatca
      301 ttgccaaacg catgaacaag aaacagcaga tgcgctggaa cagggcgacg gtgcagccct
      361 tcctcgacgt gcgaacagcg gtgctgaacg atacgctcga ggacgccttc cgtcatcgct
      421 atcccggctt tcaccccgcg aacggcaatg aagcagtcgc gttggcgggg taatgaaccc
      481 cacaggattg aatgctctcg gcggctgcat gggctgaccg cgctgcaggc gcttcgcgcc
      541 gcactggcgg gggagcccgt catgcgctcg gggtgacgtc ggcgcgatgg aggtgagtaa
      601 ttacccgcag tgagcaccgt ggcacgttca ttaccagtcg gaggttagca tctccggaca
      661 cctacgctgc aaccacagcg gcatcgtcga caggagaaat cattacgaga cttaagcggc
      721 aactatcttg acacgcaccg cccaggcctc gttatgccgc aatggtcggc ttgatcagga
      781 cggcatggac cgtggctttc gcgccgtcgt catgcgtcag cgccagttgc gcccgcccgc
      841 gatgtccggt ttgccaacct ggcacgagcc ctcgtcaggc catccccagt tcttcgacgt
      901 gttggcccac cagttccatc ccatcaggac gtcgtcgttg tcccggcaga agtcgttcca
      961 gtccgcccac ttgtcctggg cttgcggcag actgctgcac ccgccgacgc ccgcgtcgac
     1021 ggccacctcg ccgatgtgga ccttgaggcc atggcccctg gcccagccga ccaggtcatc
     1081 gcacgccttc cgcatggcgt ccgctggctt ctcggcggcg gcctcgccgg gcaagatccg
     1141 cgtgccttcg tagcaatgga ccgagatggc gaggttgcct agcggatcgg caaggccttt
     1201 ccagagggcg ctctccggat cgcccaggtc ggcctggctc ctgtcgaggc gggccgaggc
     1261 gaagcccatg ccttcgacga ggatggtgtt ctggttgccg gcatcggccc tgatcgcatt
     1321 gatcgcctgt tgcgcgcagg cttgccaccc ggcgaacgaa attccgacgt ccgctccgcc
     1381 cacggcctca tgcggctcgt tgatgagatc gaacgccagc ctgtccggcc tggcggcgtc
     1441 ggcagggaac ctgggatccg gcgggaagcc cgcggcgatg ctggcgttga tggcggtcgc
     1501 gaacttgccc cagaagtcag cgaagtgctc cggctcgaag ccgaggccgc ggtaggtgat
     1561 gtctgtattg ctcgaggcgg gattgtgctg ccaggggcag actgcgacgt agatgccgcg
     1621 gtctatgagg cggatgacga gctggaccag atcagtccaa taaatctggc gtccaccggt
     1681 cgccacgggg atcgcgccca tggcgccggc cgcgactggc ggcgctggca gcgggttggg
     1741 gttgtcctgg atggcctccc agcagatcat cacccggacg gcccgcatcc ccatgctggt
     1801 ggcgtaccaa tcaagcagcg catcgtctgt gagcggatag gttgtccccc ggattggctc
     1861 cgggccgcct tcccagaacg gcgtctccat gccgacatgg ttcacgccat tccggcgcga
     1921 acccagcatg tggtcctttg gaaaaggcat ccgaaacctc ccctggctta ttggatgctg
     1981 gcagaacctt cggatcttgt tcggccaccg gcatcgcgtt gatttttcgc aacgcatgtc
     2041 cagtcatgcc tgcgagaata gttccagtct ctgattgcac taaaatcgat ttggcgtgag
     2101 aagcagcctg gagcatgcga atcggcaagg gcgtgaaatt tccgtcgatt ggt
//
LOCUS       DASWQN010001133         4127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2484760, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001133 DASWQN010000000
VERSION     DASWQN010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484760"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..835)
                     /locus_tag="VGC82_19405"
     CDS             complement(44..835)
                     /locus_tag="VGC82_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0425494.1"
                     /translation="MDSMLVFDRLAVRRHRDRAAKTVHRVADVLRDAAERLVDRLGDT
                     NRRFVDALDVGGRGVVAPLLRERGMRVVSCDLSPAMAALSGGPCLAADEECLPFARAS
                     FDLIVASLSLHWINDLPGALLQLRQALRPEGLLLASLPALGTLSELRTALTEAEAELV
                     GGVSPRVSPFPELRDCAHLLQRAGFSVPVADLEDIRLLYADPFALLTDLRAAGETNAI
                     RERSQSSPVRALFPAALARLPMDGERFGVSLRMAVMTGWAPADRA"
     gene            917..2521
                     /locus_tag="VGC82_19410"
     CDS             917..2521
                     /locus_tag="VGC82_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0425495.1"
                     /translation="MNHISPDTLFGWITDGQELALLDAREDGAFGASHLFWAVPCGLA
                     RKEIRARALLPRPTVRVCCVDDGSGLAETLGTWLESIGCTDVSVLDGGTKAWEAAGYV
                     LFSGVNVPSKAFGEWVEHHYGTESVDATELKAMIDSGRDMVVLDSRTLEEFTRMSIPT
                     GISVPGGELAYRIADMVPDPNTLVVVNCAGRTRSIMGAESLRRAGVPNKVVALRNGTM
                     GWELAGLRCERGRTEKFEPGTPKTAALALQRAKAFAEQSGVGVIGSLDLSRFEDDPDR
                     TLYILDVRDPAEFRAGHRPGSRNAPGGQLVQGTDNWVGVRNARIVLIDDTGVRARMSA
                     AWLRQMGHRDVFVVEGGLEAVRTTGSDAIPVPELAAPVDLIDVTQLVHLLDTGTGTLV
                     LDVGRSVDFRDGHIPGAVWGVRARLDAVRSQLAAARHVVITSPDGMLARLAVAEVKGL
                     TRADVHVLEGGTDAWHAFGRPLVKDRTMPPDDACIDFYLRPYDRNSGVQEAMNAYLSW
                     EIDLVHQIERDRTVVFGIPGETQEAA"
     gene            2529..3281
                     /locus_tag="VGC82_19415"
     CDS             2529..3281
                     /locus_tag="VGC82_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY0425496.1"
                     /translation="MLARAGWIWNRALDLVLPPRCAACDNPVETHGQLCASCFGRTNF
                     ISAPFCSRCGVPLASVGQGGADVVCPVCHEHAPMFRHGRAALRYDEQARRLILPLKHA
                     DRVELASVLAPMMVRAGVALLARADVLVPVPLHRRRLFQRKYNQAAVLAFAVGRLADR
                     PVSPDALIRTRHTTPLDEKSPEERRREVTGSVGVRPSRVGQVAGKTVLLVDDVMTSGA
                     TANACAATLLEAGAVAVDVLVAARVPDPRLKS"
     gene            3393..3869
                     /locus_tag="VGC82_19420"
     CDS             3393..3869
                     /locus_tag="VGC82_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425497.1"
                     /translation="MFNKLWASALALALAAAPITASVAQGAGGGAGSGSSGGGGASGV
                     GGASGIGGGSSGSGGLGSAGSSMGSSGSAGGLSSQSAGMGATGSTPNYPGSNSVGTPT
                     GNASGTGTGMGTGSTSMGQPSTQSSVGSPNSSSITPGGPPLAGTSNMTGTGTGTGR"
BASE COUNT          671 a         1287 c         1485 g          684 t
ORIGIN      
        1 cgaattggag gccgcgcttg aatcgaccat ttcggagggg attctaagcc cggtccgcag
       61 gcgcccagcc cgtcatcacc gccatcctca agctcacccc gaacctttcg ccgtccatcg
      121 gcaaccgggc cagtgccgcc ggaaacagcg cccggaccgg gctcgactgg gagcgctcgc
      181 ggatggcgtt ggtctcccct gctgcccgca gatctgtcag caacgcaaag ggatcggcat
      241 acagcagccg tatatcttcc aaatccgcca ccggaacact gaatcccgcg cgttgcagca
      301 gatgcgcgca atcccgcagt tccgggaacg gagacactcg cggcgacacc cctccaacca
      361 gttccgcctc cgcctcggtc agcgcggttc gcagttcgga cagggtgcca agcgccggca
      421 ggctggccag cagaaggccc tcgggccgca gcgcctggcg cagttgcagc agtgcgccgg
      481 gcaggtcgtt gatccagtgc agcgacaggc tggcgacgat caggtcgaag cttgcccggg
      541 cgaagggcag gcactcctca tccgctgcga ggcaaggacc gccgctcaaa gcggccatgg
      601 cgggcgacag gtcgcaactg acgacgcgca tgccccgctc gcgcagcagt ggtgcgacga
      661 cgccgcggcc gcccacgtcc agtgcgtcca cgaaccggcg gttggtgtcg cccagccggt
      721 ccaccagccg ttcggccgca tcccgcagca cgtccgcgac ccggtgtacc gttttcgcgg
      781 cgcggtcgcg gtggcggcgg accgcaagac ggtcaaatac cagcatggag tccatgcacc
      841 ggcatgtgtg ccgacgaagc ggcgttgcca agccgtctgc ctcggctcta ccgtccgggc
      901 aaggaggatt tccccgatga accacatcag ccccgacacc ctgtttggct ggatcaccga
      961 tgggcaggag cttgccctgc tggatgcccg cgaggatggg gccttcggcg cctcgcatct
     1021 gttctgggcc gtaccttgcg gcttggcccg aaaggaaatt cgtgctcggg cgctgctgcc
     1081 ccggccgacg gttcgggtgt gctgtgtcga tgatggcagt gggctggccg agaccttggg
     1141 aacgtggctg gaatcgatcg gctgcaccga tgtttcagtc ctggatggcg gcaccaaggc
     1201 gtgggaagcc gccggatacg tgctgttcag cggcgtcaac gtgccatcca aggcgttcgg
     1261 cgagtgggta gagcatcact acggcacgga aagcgtcgat gcgaccgaac tgaaggcaat
     1321 gatcgattcc gggcgcgaca tggtggtgct ggacagccgc acgctggagg aattcacccg
     1381 gatgtcgatc cccaccggca tcagcgtgcc gggaggcgaa ctcgcctacc gcatcgccga
     1441 catggtgccc gatccaaaca ccttggtggt ggtcaactgc gccggccgca cgcgcagcat
     1501 catgggtgcc gagagcctcc gccgcgccgg cgttcccaac aaggtggtgg cattgcgcaa
     1561 cggcaccatg ggctgggaac tggcaggcct gcgctgcgag cgtggacgga ccgagaagtt
     1621 cgagcccggc acgccgaaga ccgcggcact ggcacttcag cgggccaaag cgttcgccga
     1681 acagtcgggg gtcggcgtca tcgggtcgct ggacctgagc cggttcgagg acgatcccga
     1741 tcgtacactt tatattctgg acgtccgcga cccggccgaa ttccgcgccg ggcaccggcc
     1801 gggtagccgt aacgctcccg gtggtcagtt ggtgcaggga acggacaatt gggtcggcgt
     1861 gcgcaacgcc cggatcgtgc tgatcgacga caccggcgtg cgcgcccgga tgtcggcggc
     1921 gtggctgcgc cagatggggc accgcgacgt gttcgtcgtc gaaggcggcc tggaggcggt
     1981 caggacgacc ggttccgacg cgattcccgt tccggaactg gctgcgccgg ttgacctgat
     2041 cgacgtaacc cagctggtgc atctgctcga caccggcacc ggcacgctgg tgctggacgt
     2101 tgggcgcagc gtcgatttcc gcgacgggca tatacccggc gccgtctggg gtgtccgggc
     2161 tcgtcttgac gccgtccggt cgcagcttgc cgccgcgagg catgtcgtga tcacctcgcc
     2221 cgatggaatg ttggcacggc tcgcggtggc ggaagtgaag ggccttaccc gggcggacgt
     2281 gcacgttctg gaagggggca cggacgcatg gcatgcgttc ggacggccgc tggtcaaaga
     2341 tcggacgatg ccgccggacg acgcgtgcat cgacttctat ttgcgcccgt acgaccggaa
     2401 ttccggcgtt caggaggcga tgaacgccta cctgtcgtgg gaaatcgatc tggtgcatca
     2461 gatcgagcgc gaccggacgg tggtgttcgg catccccggg gaaacccagg aagccgcctg
     2521 atttggcggt gctggctcgt gcagggtgga tctggaaccg ggcgctcgat ctggtgctgc
     2581 cgccgcgatg tgccgcatgt gacaacccag tagaaactca cgggcagctt tgtgcttcct
     2641 gtttcggccg gaccaatttc atcagtgcgc cgttctgctc gcgctgcggc gtgccacttg
     2701 cctcggtcgg gcagggcggg gccgacgtcg tgtgtccggt ttgccacgag catgcgccga
     2761 tgtttcgcca cggccgtgcg gcattgcgtt atgacgaaca ggcacgccgt ctgattctgc
     2821 ccctgaagca cgcggatcgc gtggaactgg cctcggtgct ggcgccgatg atggttcggg
     2881 caggtgtggc gctgctggcg cgggcggatg tgctggtgcc ggtgccgctg catcggcggc
     2941 ggttgtttca gcgcaagtac aaccaggcgg cggtgctggc gttcgccgtt ggaaggctgg
     3001 cggaccggcc cgtgtcgccc gacgcgctaa tccggacccg gcacaccacg ccgctggatg
     3061 agaagtcccc cgaggagcgg aggcgtgagg tcacggggtc ggtgggggtg cggccatccc
     3121 gggtagggca ggtcgcggga aagacggtat tgctggtgga tgacgtgatg acatccggcg
     3181 cgacggcaaa tgcctgcgcg gccacgctgc tggaagccgg ggcggtggcc gtcgatgtcc
     3241 tggtcgcggc gcgggtgccc gatccgcggt tgaagtcgtg accctccgta ttttggggcg
     3301 catcgaaaca acgtgaggca accgctgtcg ccacccctcg cgcgttgagg ccgttgtcgc
     3361 cgcactggtg cgcgacaagg aaaggattga ggatgttcaa caagctgtgg gcctcagccc
     3421 tggcgctggc cctggcggcg gcgccgatca ccgcctcggt ggcgcagggc gcgggaggcg
     3481 gagcaggcag tggaagctcc ggtggcggcg gcgcttccgg cgtgggcggc gcttccggaa
     3541 ttggtggcgg gtcctcgggt tcaggcggtt tgggttcggc gggctcctcc atggggtcca
     3601 gcggttcggc ggggggcttg agttcgcaaa gcgccggcat gggcgcgacc ggctcgacgc
     3661 cgaactatcc gggcagcaac agcgtcggca cgcctaccgg aaatgcctcg ggaaccggaa
     3721 ccggcatggg cacaggcagt accagcatgg ggcagccgag cacgcaaagt tcggtggggt
     3781 cgccgaacag ctcaagcata acgcccggag gaccgccgtt ggcggggacg tcgaacatga
     3841 cgggaactgg gacaggaacg ggtcgatagg ttcgcaggcc gtgggttagc tggtcttaga
     3901 ttggcgagtc gataaatcgg cgggtcgata aatcggcggg tcgatagatt ggcgggccgg
     3961 aagcggtcgc accaccggaa gctgcgccgc ggccaaattc gccgcggcgc gggtcgggag
     4021 aaagcttcgc taccaggcgg tggcgaagga ttccgggccc gctccttcga ccggaaggct
     4081 tttgccgcgg gtcagggttt tgccgtccca gcggaacacc tcgatca
//
LOCUS       DASWQN010001134         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_375125, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001134 DASWQN010000000
VERSION     DASWQN010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375125"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /gene="mntR"
                     /locus_tag="VGC82_19425"
     CDS             complement(<1..403)
                     /gene="mntR"
                     /locus_tag="VGC82_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HEY0425498.1"
                     /translation="MVGNPIRPASTLPEEPTQASRFGKARSAQSGALLEDYVELISDL
                     LTSDGEARPIDIARRLGVAHATAIKTIGRLKREGLAVAKPYRGVFLTETGESLASRVR
                     ARHRVVVDLLLAVGVPFESAESDAEGIEHHVS"
     gene            complement(441..1727)
                     /locus_tag="VGC82_19430"
     CDS             complement(441..1727)
                     /locus_tag="VGC82_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY0425499.1"
                     /translation="MSQAVIRPSLSHRTAMAGEIALSGGRSGLRGLLPFVGPAVVASI
                     AYMDPGNFATNIQAGARHGYMLLWVVLLANIIAMLFQALSARLGIVSGQSLAALSRVH
                     FPKPLVVAMWGASEVAAMATDLAESIGAAIGISVLFHLPLLAGLLITFAITWGLLTFQ
                     SRGFRPIELIVTGFVGIISLAYLIELFIAPPEWGQFFFHTVVPQLNGSDSVTLAVGIV
                     GATVMPHAIYLHSALVTERVKTRSDQERWRLIRFSNLEVLIALGLAGLVNMAMVSMAA
                     TMFHQGHSDVGEIETAYHTLLPLMGVVAAGAFMTSLLASGLSSSVVGTMAGQVIMQDF
                     VGFRIPLWVRRAVTMVPAVVVVAMGVQATDALVISQVVLSLVLPVPMIALLVLIRRPG
                     VMAGFAIGRPMQMLAAAATIVVLALNVVLLLQYIGD"
     gene            1891..>2297
                     /locus_tag="VGC82_19435"
     CDS             1891..>2297
                     /locus_tag="VGC82_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEY0425500.1"
                     /translation="MRHFLDFEKPIAELEGKIEELRRMSEPDGINIADEVTRLTTVAD
                     KQLRSTYLRLTPWQKTLVARHPDRPKAQDYIDNLITEFTPLAGDRAFADDAAVLGGIG
                     RFRGRPVMVLGTEKGTDTDSRVKHNFGMARPEG"
BASE COUNT          464 a          801 c          682 g          350 t
ORIGIN      
        1 ccgatacgtg gtgttcgatg ccttccgcgt ccgactccgc cgactcgaac ggcaccccga
       61 ccgccagcag caaatcgacc accacgcggt gccgggcgcg cacgcgtgag gccaggctct
      121 caccggtttc cgtcaggaag acgccacggt atggcttggc caccgccagg ccttcccgct
      181 tcaaccgccc gatcgtcttg atcgccgtcg cgtgcgcgac gcccagccgg cgggcgatat
      241 cgatcggccg agcctcgccg tcgctggtaa gcaggtcgga gatcagttcg acgtagtcct
      301 ccagcaacgc gccggactgg gccgaacgcg ccttgccgaa ccggctggcc tgggtcggtt
      361 cctccggcag cgtggaggcc gggcgaattg ggttccccac cattgggtct gacataaccc
      421 ccctttgagc caggatcagc ctaatcgccg atgtattgca acagcaggac cacgttcagc
      481 gccagcacga caatggtcgc cgcggccgcc agcatctgca tcggtcgtcc gatggcaaat
      541 cccgccatca cgccgggacg ccggatcagc accagcagcg cgatcatcgg caccggcaat
      601 accagactga gcaccacctg actgatcacc agcgcgtccg tggcctgcac acccatggcc
      661 acgaccacga ccgcgggcac catcgtcacc gctcggcgca cccatagcgg gattcggaac
      721 ccgacgaaat cctgcatgat gacctggccc gccatcgtcc ccaccacgga actcgacagc
      781 cccgacgcca gcagcgacgt catgaacgca ccggccgcca ccacgcccat cagcggcagc
      841 agcgtgtgat acgcggtctc gatctcgccg acgtcggagt gtccctgatg gaacatcgtc
      901 gccgccatcg acaccatcgc catgttgacc agcccggcaa gccccagggc gatcagcacc
      961 tccaggttcg aaaaccggat caaccgccac cgttcctgat cggagcgtgt tttcacccgc
     1021 tccgtcacca aagcggaatg caggtagatc gcatgcggca tgaccgtagc gccgacgatg
     1081 cccaccgcca gcgtcacgct gtccgatccg ttcaactgcg gcacgacggt gtggaagaag
     1141 aactgccccc actccggggg cgcgatgaag agctcgatca gataagcgag gctgatgatg
     1201 ccgacaaacc cggtgacgat aagctcgatc ggccgaaagc cgcgcgactg gaaggtcagc
     1261 agcccccagg tgatagcgaa ggtgatcagc aatccggcca gcagcggcag atgaaacagc
     1321 acactgatgc cgatggcggc cccgatcgac tccgcgaggt cggtggccat tgccgccacc
     1381 tcacttgctc cccacatcgc gacgaccagc ggtttgggga aatgcacgcg acttagcgcg
     1441 gccagggatt gcccgctgac gatgccgagg cgcgccgaca gcgcctggaa cagcatggcg
     1501 atgatgttgg ccagcagaac gacccacagc agcatgtagc catgccgggc gcccgcctgg
     1561 atgttggtcg cgaagttgcc gggatccatg taggcgatgg aggcgaccac cgctggtccg
     1621 acaaacggca gcaggccgcg caggccgctt ctgccgccgc ttaaggcgat ctcaccggcc
     1681 atggcggtcc gatgcgacag gctggggcgg atgaccgcct ggctcatcgg cagcccacgc
     1741 cggatagaca tgaagcatta gtcattattc gcgcacccta ccacacagtg tagccgatgc
     1801 tacacattgg cgccggcggc ttgttgtcaa caccttccca cggccccaac cgcttccctt
     1861 gaccgcgaac tgttgctaaa gccccgcccg atgcgccatt tcctcgactt cgaaaaaccc
     1921 atcgccgaat tggaaggcaa gatcgaagag ctccggcgca tgtcggagcc cgacggcatc
     1981 aacatcgccg atgaggtcac ccgcctgacc acggttgcgg acaagcaatt gcgctcgacc
     2041 tatctgcgcc tgactccgtg gcagaaaact ttggtggccc gccatcccga ccggcccaag
     2101 gcgcaagact atatcgacaa cctgatcacc gagttcacgc cgctcgcggg agaccgggcg
     2161 ttcgccgacg atgcagcggt attgggcggc atagggcgct tccgcggccg gcccgtcatg
     2221 gttctgggaa ccgaaaaggg aaccgacacc gattcgcgcg tgaagcacaa ttttggcatg
     2281 gcgcgtcccg agggcta
//
LOCUS       DASWQN010001135         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2485123, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001135 DASWQN010000000
VERSION     DASWQN010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2485123"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VGC82_19440"
     CDS             <1..438
                     /locus_tag="VGC82_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425501.1"
                     /translation="RIDPETTSSVAHGWHVAAPAITRNGFRQFHRASAVGRSVVDEQP
                     QPTKPRKPSISPAAGGAAMDTVQAKACRNHTASVESRMKTAFIAGRQNWHRDTDGKGP
                     SHRIQCRLVANRPNIPSAPDRSKILLRGTLWHSFFLRVENKEG"
     gene            575..>2695
                     /locus_tag="VGC82_19445"
     CDS             575..>2695
                     /locus_tag="VGC82_19445"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0425502.1"
                     /translation="MHLDLKLWRMTAGQRGRIVLSAALGLLALTIGIARFVFLGRFLA
                     GVFQGAFLIAPILGAAAAILLRAWLDHHRTMIAHRTAARVQENLRGRLFDKIIALGPA
                     WFGAERTGGVMLSMVDGVEQLQSFFGQYLPQVTIAVCAPVAIFAFIAWWDVPVATVML
                     AAALFTLVLPATVHRKTATASRTRQNAFKSFGEEFLDAVQGLPTLKAFGQSAAYGRML
                     AAKARALSDSTFWVLSLNVLTRGFTDLGTAFGSAAALALGAWRVRHGDMSLEALLIVL
                     MAGTEIFRPLRDLRSVLHQGMNGQAAANGINALLDTPVAAPTGRPAPPLTPEISFDNV
                     AFAYPGGRQAAHAGLTFQVAAGERVGIVGPSGAGKSTIVRLLSRLHEAQSGAIRIGGR
                     DVRDLDPAQLRTMIAVVSQDTYLFHGTVEENLRLGSPDASHAELVAAAAAANAHEFVS
                     ALPDGYQTVIGERGTQLSGGQRQRLAIARALLRNAPILVLDEALSSVDAENEAIIQQA
                     LDRLMIGRTSLILAHRLSSVIGADRILVLSNGAVVQSGHHGDLIRQDGPYRDLMGSQL
                     GEREGLIAALPDTENTRRDTPSTTAVVGNLSDDAAQVTWPATIRTLLRVVRPYRTQLA
                     VTVLTGIGRVVAFIGVSVLGALVLAAVRNGTPTGTLIAALLITAPLAGILHWLESWIA
                     HAMAYALLADMRVDLFRKLDALAPA"
BASE COUNT          444 a          924 c          832 g          495 t
ORIGIN      
        1 cggatcgacc cggaaacgac ttcatcggtg gctcatggct ggcacgttgc tgcgcctgca
       61 atcaccagga atgggtttcg acaattccat cgcgcgtcgg ctgttggtcg ttcggtagtc
      121 gatgagcagc cgcaaccgac gaagcctcgc aagccgtcga tcagtcctgc ggccggaggt
      181 gcagcgatgg atactgttca agcgaaggca tgtcgaaatc atacggcgag tgtcgagagc
      241 cgcatgaaaa ctgccttcat tgctggacga cagaactggc accgcgatac cgacgggaaa
      301 ggtccatcac atcgcatcca gtgcaggtta gtcgccaatc ggccaaatat tccgtcggct
      361 cctgatcgca gcaagatcct gctccgtggc actttgtggc actcattttt tctccgtgtt
      421 gaaaataagg aagggtgaac gcactcgctg attgcaccca accccggcta cgctcagacc
      481 actcccagca agcagtacct cctccgcacc tcagcaccgc attagcggcg cccggcgatg
      541 gacgccgggc cgtagctaag gcaccatcat tgccatgcat ctcgacctga agctgtggcg
      601 catgaccgct ggccaacgag gccgcattgt cctgtcagcc gcgttgggcc tgctcgcctt
      661 gacgatcggg atcgcccgct tcgtcttcct tggccgcttt ctggccggtg ttttccaagg
      721 cgcgttcctg atcgccccca tcctcggcgc cgccgcggcg atcttgctcc gcgcctggct
      781 ggaccaccac cgcaccatga tcgcgcaccg cactgcagca cgggttcagg aaaacctgcg
      841 cggccggctg ttcgacaaga tcattgcctt ggggccagcc tggttcgggg cggagcggac
      901 cggcggcgtc atgctgtcga tggtcgatgg cgtggagcag cttcagtcct tcttcggtca
      961 atacctgccc caggtgacga tcgcggtttg cgcgcccgtc gccatctttg ccttcatcgc
     1021 gtggtgggac gttcccgtgg cgaccgtcat gctggccgct gccctgttca ccctggtcct
     1081 gcccgccacc gtccatcgca agacagccac ggcatcccgc acgcgccaaa acgccttcaa
     1141 atcgttcggc gaagagttcc tggatgccgt ccaaggcctg cctacgctga aagcctttgg
     1201 ccaaagcgcc gcatatggcc gaatgctggc ggcaaaagcc cgggccctgt cggacagcac
     1261 attctgggtc ttgtcgctga acgtgctgac gcggggcttt accgacctcg gcaccgcttt
     1321 cggatcggcc gccgccctcg cactcggtgc atggcgcgtg cgccacggcg acatgagcct
     1381 ggaggcgctg ctgatcgtcc tgatggcagg caccgagatc tttcgcccgc tgcgcgatct
     1441 gcgcagcgtc ctgcaccagg gcatgaacgg ccaggccgcg gccaacggca tcaacgcact
     1501 gctggacacg ccggtcgccg ctccgactgg ccggcccgcg ccacccctga cgccggagat
     1561 cagcttcgac aacgtcgcgt tcgcctaccc gggaggccgg caggctgccc atgccgggct
     1621 gacgttccag gtcgccgccg gcgagcgtgt cggcatcgtc ggaccgagcg gggccggcaa
     1681 atcgacgatc gttcggctgc tgtcgcggct gcatgaggcg cagagcggtg ccatccgcat
     1741 cggcgggcgg gatgtgcgcg acctcgaccc ggcgcagctc cgcaccatga tcgccgtcgt
     1801 ttcccaggat acctacctgt ttcacggcac tgtcgaggag aaccttcgtc ttgggtcccc
     1861 cgatgccagc cacgccgaac tagtcgccgc cgccgccgcc gccaatgcgc atgaattcgt
     1921 cagcgccctg cccgacggct atcagaccgt gatcggcgag cgcggcaccc agttgtccgg
     1981 cggccagcgc cagcgcctgg caatcgctcg ggcgctgctg cgaaatgcgc cgatcctcgt
     2041 gctggatgag gcactttcct cggttgacgc cgagaacgag gccattatcc aacaggcgct
     2101 ggaccggttg atgattggcc gcacctcctt gatcctggcg caccggctat ccagtgtgat
     2161 cggcgccgac cggatcctgg tgttgagcaa tggcgccgtg gtgcaatccg gccaccatgg
     2221 cgacctgatc cggcaggacg ggccctatcg cgacctgatg ggatcgcagc tcggggaacg
     2281 ggaaggcctc atcgcggcgt tgccagatac tgaaaacaca aggcgggaca cgccctccac
     2341 caccgctgtc gttggcaacc tgtcggatga tgccgcccag gtaacgtggc cggcgaccat
     2401 ccgcactttg ctgcgggtcg tccggccata ccggacccag ctggctgtta ccgtgctgac
     2461 cggaatcggc cgcgtggtgg ccttcatcgg cgtcagcgtc ctcggcgcac tggtgttggc
     2521 cgcggtgcgc aacggcaccc cgaccggcac gctgatcgcg gccttgctga tcaccgcgcc
     2581 gctcgccggg atactgcact ggctggaatc ctggattgca cacgctatgg cctatgcgtt
     2641 gctggccgac atgcgggtcg atctgttccg caaactcgac gcgctcgcgc ccgcc
//
LOCUS       DASWQN010001136         3666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_111196, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001136 DASWQN010000000
VERSION     DASWQN010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111196"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VGC82_19450"
     CDS             complement(<1..511)
                     /locus_tag="VGC82_19450"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0425503.1"
                     /translation="MALLRLQVEWGVDEALDADPIDRFTAIDPPRPIEAGSALRARPA
                     PALPMGSQAERALALAMQAATLDELRTTLASFDGCALRDTASNLVFAEGNPSSPILII
                     GEPPGREEDRSGHPFAGPEGQLLDQMLASIGLTRSELMLTPLLPWRPPGGRPPSPAEI
                     AICQPFLHRL"
     gene            complement(516..1130)
                     /locus_tag="VGC82_19455"
     CDS             complement(516..1130)
                     /locus_tag="VGC82_19455"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0425504.1"
                     /translation="MAKLSKLQTATLPDAVLDRRQAVPALIEHPQDFLMVAGLAGTAW
                     ELSALTRESPSLFGLGGAMGAAPSIGLGLALAQPKRRVLVATGDGELLMNVGALATIA
                     LQNPPNLTIICVDNCHYGETGNQDSHTGLGVRLDVIAAGCGFPVVHVINTVDDMAEGR
                     RLVREGGGLTFILLRVTERPATHDGKKNLVPHVERDRFRAALLG"
     gene            complement(1144..1659)
                     /locus_tag="VGC82_19460"
     CDS             complement(1144..1659)
                     /locus_tag="VGC82_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HEY0425505.1"
                     /translation="MADDETWPNALYDLLRANHVTQFCYVPDAGHRVLIDRSLADPDV
                     HSIPLTTEEEGVALVAGADLGGQRAVLLMQSSGVGNCINMLSLTQGCRFPFLTLISMR
                     GEFGEGNPWQFAMGQAVEKVLQAMGVIVLRAEQPADVVPCASAAITMAFQSGQQVAVL
                     LTQRLIGAKKF"
     gene            complement(1883..3106)
                     /locus_tag="VGC82_19465"
     CDS             complement(1883..3106)
                     /locus_tag="VGC82_19465"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0425506.1"
                     /translation="MWVRRAALGIALLAPVASASVAIGSARADVPDTVKVGILNDMNG
                     PFADQSGRGSVVAAQLAAENFAAEGGGFKVEILSADHLNKPDVAGQIVRQWVDRDGVA
                     AVADSVNSSVGLAVNTIMAEKHRTFVATNVGTSDLTGKFCQPTTVQWTMDTYAMGNTM
                     ARAMVAQSADSWYYISFDYALGAALERDSAAVLTSLGGKVLGSVKHPLGTTDFSSYLL
                     QAQASGAKVVALADTGSDLINAVKQAAEFGLTRKQILAGLFTQIVDVDSIGLQAAQGL
                     TVTEAFYWDLNDNTRSFAARFAQRFGGRVPTANQAGVYSSVLAYLQAVKAANTIDGEA
                     VVAQMRKQPIEDKLFGSVTVRPDGRAVHDMYTFRVKTPEESKSRWDLYKVLARVPGNE
                     AFRPLNQGGCRLVTN"
     gene            complement(3140..>3666)
                     /locus_tag="VGC82_19470"
     CDS             complement(3140..>3666)
                     /locus_tag="VGC82_19470"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEY0425507.1"
                     /translation="DLLRPLGVQVVSAGDLGLPEPDEDAADFAGNARIKAVAAATASG
                     LPALSDDSGFCVAALDGAPGVHSARWAGPAKDFAAAMAAVWQRIGENPDRRAWFVAAL
                     CLAWPDTHTQTFVGRIDGTVVWPPRGDKGFGYDPMFLPAGATRTFGEMDPDEKHAVSH
                     RARAFVQVMTSCFG"
BASE COUNT          652 a         1149 c         1214 g          651 t
ORIGIN      
        1 tcagccggtg aaggaacggc tggcatattg cgatctccgc cggagatggc ggccgcccgc
       61 ctgggggacg ccatggcagc agcggagtca gcatcaattc gcttcgcgtc aggccgatgc
      121 tggccagcat ctggtcgagc aactggccct cgggtccggc gaacggatga ccgctgcggt
      181 cctcctctcg tcccgggggt tcaccgatga tcaggatcgg actggaaggg ttgccttcag
      241 cgaacacaag attgctggcg gtgtcgcgta aggcgcagcc gtcgaagctg gccaaagtgg
      301 tgcgcagttc gtcaagcgtg gcggcctgca tcgccagggc aagcgcccgt tcggcctggc
      361 tgcccatggg cagcgcggga gcaggacgcg cacgcaaggc cgatccggcc tcgattggtc
      421 gcggcgggtc gatggcggtg aaccggtcga tcggatcggc gtcaagcgcc tcgtcgacgc
      481 cccattcgac ctgcaggcgc agcagtgcca aggcatcatc caagcagggc agcccggaaa
      541 cggtcccgtt ctacatgagg gacgaggttc ttcttgccat cgtgggtggc cggacgttcg
      601 gtgacccgga gcaggatgaa cgtcaggccg ccgccctcgc ggaccagccg gcggccttcc
      661 gccatgtcgt caacggtgtt gatcacatgc acgaccggga agccgcagcc ggcggcgatg
      721 acgtcgagcc ggacgccgag gccggtgtgg ctgtcctggt tgccggtttc cccgtagtgg
      781 cagttgtcca cacagatgat ggtcaggttc ggaggattct gcagcgcgat cgtcgccaac
      841 gcgccgacgt tcatcagcag ttcgccgtcg ccggtcgcca ccagcacgcg ccgcttcggc
      901 tgcgccaggg caaggcccag cccgatcgac ggtgcggcac ccatcgcccc gccaaggccg
      961 aacaggctgg gggattcccg tgtcagcgcg gaaagttccc acgctgtgcc ggcgagaccg
     1021 gcgaccatca ggaagtcttg cggatgctcg atcaaagcgg gcaccgcctg acggcgatcc
     1081 agcaccgcgt cgggcaaagt cgctgtctga agcttgctca atttcgccat atcgggtgtc
     1141 ccgctaaaat ttcttggcgc cgatcaggcg ctgggtgagc agcacggcga cctgctgacc
     1201 ggactggaac gccatggtga tggcggcgct tgcgcaggga acgacatccg ccggctgttc
     1261 cgcccgcaat acgataacgc ccatcgcctg cagcaccttc tcgaccgcct ggcccatcgc
     1321 gaactgccag gggttaccct cgccgaattc tccgcgcatc gaaatcaggg tcaggaacgg
     1381 gaagcggcag ccctgggtca gcgacagcat gttgatgcag ttgccaacgc cggaggactg
     1441 catcagcagc accgcccgct ggccgccgag gtcggcgccg gcgaccagcg ccacgccttc
     1501 ttcctccgtg gtcagcggga tcgagtgcac atcgggatcg gcgagcgagc ggtcgatcag
     1561 caccctgtgc cccgcatcgg gtacgtagca aaactgggtg acgtggttcg cccgcaacag
     1621 gtcgtacagc gcattgggcc aggtttcatc gtccgccatg tgttttccta gaaaatttcc
     1681 agcctgggtg tgcaggctgg aaatgctcta tctccttgtt taatcgcatg attcacacgc
     1741 ttcggacgtt ccgcccggcg tgatcatggt cttgtctaat cgcatgattc acacgcttcg
     1801 gacgttccgc ccagcgtgat catggtcttg tctaatcgca tgattcacac gctccggacg
     1861 ttccgcccgg cgtgatcatg gtctagttcg tgaccaatct gcatccaccc tggttcagcg
     1921 gccggaaggc ttcgtttcct ggcactcggg cgagaacctt gtagagatcc cagcgtgact
     1981 tcgactcctc cggcgtcttt acccgaaacg tgtacatatc atgcaccgct cggccatccg
     2041 gacgcaccgt gacggagccg aacagtttgt cctcgatcgg ctgcttgcgc atctgggcga
     2101 cgacggcctc accgtcgatc gtgttggcgg ccttgacggc ctgtagatag gccagcacgg
     2161 acgaatagac cccagcttgg ttcgcggtcg gaacacgccc gccgaagcgc tgcgcgaaac
     2221 gcgcggcgaa gcttcgagtg ttgtcgttga ggtcccagta aaaggcttcg gtgaccgtca
     2281 gtccctgggc ggcttgcagg ccgatggagt cgacatcgac aatctgggtg aacaagccag
     2341 ccaggatctg tttgcgcgta aggccgaact cggctgcctg tttgacggcg ttgatcaggt
     2401 cggagccagt gtcggcaagc gccacgacct tggcgccgga agcctgcgcc tgcagcaggt
     2461 aggacgaaaa atcggtggtg cccagcgggt gcttgaccga ccccaggact ttgccgccga
     2521 ggctggtcag cacggcggcg gagtcacgct ccaatgccgc acccagcgcg tagtcgaagc
     2581 tgatatagta ccaggaatcg gctgactgcg cgaccatggc acgggccatg gtgttgccca
     2641 tggcataggt gtccatcgtc cactgcacgg tcgtgggctg gcagaacttg ccggttaggt
     2701 cggacgtgcc gacattggtg gcgacgaagg tgcggtgctt ttcggccatg atcgtgttga
     2761 cggccagccc gacgctggag ttcaccgaat ccgcgaccgc ggcaacaccg tcccgatcca
     2821 cccattgccg gacgatctgg ccggcgacgt cgggcttgtt gaggtggtcg gcggaaagga
     2881 tttcgacctt gaagccaccg ccctcggcgg cgaagttctc agcggccagt tgcgcggcaa
     2941 cgacggagcc tcggccggac tggtcggcaa agggaccatt catgtcgttg agaatgccga
     3001 ctttgacggt gtcgggaacg tccgcccggg cggaaccgat cgccaccgaa gcggaagcga
     3061 ctggtgccag tagcgcgatg cccagggccg cgcggcggac ccacattctc gatacctccc
     3121 gttggcggtc tcgattacat caaccaaagc aggacgtcat tacctgaacg aaggctcggg
     3181 ctcgatggct gaccgcatgt ttttcgtccg ggtccatttc cccgaaggtc cgggtcgcgc
     3241 cggccggaag gaacataggg tcatagccaa agcctttgtc gcctcgtggt ggccagacga
     3301 cggtgccgtc gatgcggccg acgaaggttt gggtgtgggt gtcgggccag gccaggcaca
     3361 acgcggcgac gaaccaggcg cgacggtcgg gattttcgcc gatacgttgc cacaccgcgg
     3421 ccatggcggc ggcgaaatct ttggcggggc cagcccagcg ggcggaatgt acgccggggg
     3481 caccatcaag ggcggcgacg cagaagccgg aatcatccga tagcgcgggc aggccgctcg
     3541 cagttgccgc cgcaacggct ttgatgcggg cgttaccggc gaaatcggcg gcgtcttcgt
     3601 caggctccgg cagcccaagg tcgccggcag agacgacctg gacaccgagt ggccgaagca
     3661 gatcct
//
LOCUS       DASWQN010001137         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2619251, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001137 DASWQN010000000
VERSION     DASWQN010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619251"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1684)
                     /gene="groL"
                     /locus_tag="VGC82_19475"
     CDS             complement(83..1684)
                     /gene="groL"
                     /locus_tag="VGC82_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308472.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0425508.1"
                     /translation="MPAKDVRFGGDARQRMMRGVDTLADAVKVTLGPKGRNVLLDKSY
                     GSPRITKDGVSVAKEIELADKFENMGAQLVREVASKTNDIAGDGSTTAIVLAQAIIRE
                     GAKSVSAGMNPMDLKRGVDKAVTALIEELKKRSKKITTQAETAQVGTISSNGDADIGK
                     MIAEAMQKVGNEGVITVEEAKGMLTELDVVEGMKFDRGYMSPYFITNAEKMTVELDNP
                     YILLNEKKLSNLQSILPLLEAVAQSGRPLLIIAEDVEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDLAVLTKGDVISEDLGIKMENVTLAMLGTAKRVLIDKENTTVVE
                     GAGKKADIQGRCRQIRAQIEETTSDYDKEKLQERLAKLAGGVAIIRVGGSSETEVKER
                     KDRVDDALHATRAAVEEGIVPGGGVALARASQILQKMKADNEDQRVGIEIVRRAVLSP
                     LRLIASNGGEDGAVISGKTLEKDEYNYGFDAQAGEFKDLVKAGIIDPTKVVRCALQNA
                     ASIGGLMITTEAMVGEHVDPPTHDA"
BASE COUNT          382 a          706 c          603 g          459 t
ORIGIN      
        1 gaaccatgac gcttccgggc ggagcgccca atgcttgtga agcacgcggt caaaccagga
       61 gatgggccag ccgcctcacc aatcaagcgt cgtgagtggg aggatcaacg tgctcgccca
      121 ccatcgcttc ggtcgtgatc atcaagccgc cgatggacgc ggcgttctgc aacgcacagc
      181 gcacgacctt cgtcggatcg atgatcccgg ccttgaccag gtccttgaac tcaccagcct
      241 gggcatcgaa gccgtagttg tactcgtcct tctccagcgt cttgccggaa atgacggcac
      301 cgtcctcgcc accgttggag gcgatcaaac gtagcggact caggaccgcc ctgcggacga
      361 tctcgattcc gacgcgctga tcctcgttgt cggccttcat cttctgcagg atctgggaag
      421 cacgagccaa cgcgacaccg ccgcccggca cgattccctc ctcgaccgcg gcgcgagtcg
      481 cgtgcaacgc atcgtccacg cggtccttac gctctttcac ttcggtctca gacgagccac
      541 cgacgcggat gatggcgaca ccaccggcca gcttcgccag acgctcctgc agtttctcct
      601 tgtcgtagtc cgaggtggtt tcctcgatct gcgcccggat ctgcctgcag cggccctgga
      661 tgtcggcctt cttgccggcc ccttcgacga cggtggtgtt ttccttgtcg atcagcaccc
      721 gcttcgccgt gcccagcatc gccagggtca cgttttccat cttgatgcca agatcttcgc
      781 tgatcacgtc gcccttggta aggacagcca gatcctccag catcgccttg cggcgatcgc
      841 cgaaaccagg agccttcacg gctgcgacct tcaggccgcc gcgcagctta ttcaccacca
      901 gcgtcgcaag ggcttcaccc tcgacgtctt ccgcgatgat cagaagcgga cgcccggact
      961 gagcgacagc ttccagcaac ggcaggatgg actgcaagtt ggaaagcttc ttctcattca
     1021 gcaggatgta gggattatcc agctcgacgg tcatcttctc ggcgttggtg atgaagtacg
     1081 gcgacatgta gccacggtcg aacttcattc cctcgacgac gtcgagttcc gtcagcatgc
     1141 ccttggcttc ttcgacggtg atgacacctt cgttgccgac cttctgcatg gcctcggcga
     1201 tcatcttgcc gatatcggcg tcgccgttgg acgaaatcgt gccaacctgc gcggtttccg
     1261 cctgcgtcgt gatcttcttg gagcgctttt tcagctcctc gatcagggcg gttacggcct
     1321 tatcgacgcc gcgcttcagg tccattgggt tcatgccggc cgagaccgac ttggcccctt
     1381 cgcgaatgat cgcctgggcc agcacgatcg ccgtggtgga accatcgccg gcgatgtcgt
     1441 tggtcttcga agccacttca cgcacaagct gtgcgcccat gttttcgaac ttgtcagcaa
     1501 gctcgatttc cttggccacc gacacaccgt ccttggtgat gcgcggcgat ccataacttt
     1561 tgtcgagcag cacgttgcgg cctttggggc ccagcgtgac cttcacggca tcggcgagcg
     1621 tatccacacc gcgcatcatg cgctgacggg catcgccgcc gaaccgaacg tccttcgcag
     1681 gcatttatgt atccttcccg agatctcaat catcgccggc tcgtcatccg gatcagccgg
     1741 tgaccgaggt gttgtcgaag cttggcggca ttttgatctg gcgatcggat gtgatcgcca
     1801 cgccgccgcc acgacaatgg tttcgccggc caacccctgt tagcactcat cggtttcgag
     1861 tgccagggat aagattcggc ttcggtcacc ttgtcatctg ctgaattgga gacagccgaa
     1921 agtgagcccg taggtcatgc ggatcgcgcc gaagggtcgc ttccggtgcc gtgttggggc
     1981 ccagtgtcgc ccggttccaa gcggtgtccg gcaccaactt ctgaccccga acaggcgtaa
     2041 agacgagcga cgggaaacca ggacgcatca gataaggccg cccgatcaga cccgcctgat
     2101 cagcccgatg tatcccccgc gcccgatccc atatataatg aggttttagc
//
LOCUS       DASWQN010001138         3074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1826988, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001138 DASWQN010000000
VERSION     DASWQN010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826988"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..738
                     /locus_tag="VGC82_19480"
     CDS             247..738
                     /locus_tag="VGC82_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY0425509.1"
                     /translation="MSKFPLSLASAGIAGLMPVGAPMAARADVNWTLSNIAFADGGTA
                     SGTFTTSDSGLLASWDIVTQDGANGAIYDSALGSTATGITAASFDIVSGSQDLALAFS
                     SDLTASTYATSGIAPVATGIETFVAGSRAIMAGDAIPEPVSMALFGTSLFGLGAIRLR
                     RRA"
     gene            861..2942
                     /locus_tag="VGC82_19485"
     CDS             861..2942
                     /locus_tag="VGC82_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEY0425510.1"
                     /translation="MASSIEASVCPHDCPSTCALSVEVLDGTRIGAVRGAADNSYTAG
                     VICAKVSRYAERIHHPDRLTQPLLRTGPKGAGQFRPISWTEALDRIADRFTADTAKHG
                     SEAVWPFYYAGTMGLVQRDGINRLRNVMRYSRQKMTICTSLPEIGWQAGVGASRGVDP
                     REMAQSDLIVVWGGNPVATQVNVMTHVSRARKERGAKLVVIDPYKTATAAVADLHLAP
                     RPGTDAALACAVMHVAFRDGYADHDYMRNYTDCPDALEAHLRDCGPGWASAITGIPTE
                     RIEAFATLYGQTKRSFIRAGYGFARSRNGCAAMHAVSCLPAVTGAWQHEGGGALWSNR
                     GMYHWNKTLIEGLDAQDPSLRILDMSRIGSVLTGDRTELGDGPQVHSMIIQNQNPLTV
                     CPDSNRVRRGFGRSELFVATHEQFLTETVRWSDIVLPATMFMEHDDIYQAGGHSHIQI
                     GPKLIAPPGECRSNHEVMQGLASRLGAKHRGFDMTAMELIDATLRASGYPDAKTVLER
                     RWIDAMPPFREAHFLDGFPTKDRRFHFAPDWKALGDKHAVMPGLPDQMANIEAAGPEA
                     PFRLVTAPARSFLNSTFSEMASGRKREGRPTVLMHPDDASRLGLIEGSKVRLGNVRGE
                     VVLHARISDGQQPGVLVSESIWPSECFEGGIGINALTSDDPGPPYGGAVYHDSAVWMR
                     AEAVQAALAAE"
BASE COUNT          548 a          965 c         1000 g          561 t
ORIGIN      
        1 tcgccggctg accgtccgcc atcgcgtcgc ggggggcggc caacgccgac cgtgcagtat
       61 cggcgcggtt agttagtggc ttgtcgcggt actttacaca tccgcccatt tgcatacggg
      121 cgtatgcgga tttcccaacg ccatcaacgg gtttgggatt ggcatcacct ttgcttgtgc
      181 aacattgtac ccatggtggg cgggacacgc tgtcattagc gggattggac gcaaggaatg
      241 cattctgtga gcaaatttcc cctatcactt gcctcggctg ggatagcagg cctgatgccg
      301 gtgggcgcac caatggcagc ccgtgccgac gtcaactgga ccttgtccaa catcgccttc
      361 gccgatggcg gcacggcatc cggcacgttc acgacctcgg acagcggctt gctggcatcg
      421 tgggacatcg tgacgcagga cggcgccaac ggtgccatct atgacagcgc tctcggcagc
      481 acagccaccg gcatcaccgc cgcatccttc gatatcgttt ccggttcgca ggacctggcg
      541 cttgcctttt cctccgacct gaccgcatcg acctacgcga catccggcat agcgccggtg
      601 gcgaccggga tcgagacgtt tgtcgccggc agccgcgcga tcatggccgg cgatgccatt
      661 ccggagccgg tgtccatggc gctgttcggc accagtctgt ttggcctggg ggcaatccgg
      721 cttcgtcgcc gcgcctgacc ccggcgaacc aaaggcggca tccgcagggg tgccgcttgc
      781 gttcggcgaa aacatcggcg gatcgaagat gccgcgcgcg gcattgcacg accacacgct
      841 ttctcgatac gctggcgccc atggccagct cgatcgaagc ctctgtctgc ccgcacgact
      901 gcccttcgac ctgcgccctg tcggtcgagg tcctggacgg cacacgcatc ggtgcggtgc
      961 gcggcgcggc cgataacagc tacaccgcag gggtgatctg cgcgaaagta tcgcgatatg
     1021 ccgaacgcat ccatcacccc gacaggctga cccaaccgct gctgcgcacc ggccctaaag
     1081 gtgccggcca gttccgcccg atctcatgga ccgaggctct cgaccgcatc gccgatcgtt
     1141 tcaccgcgga cacggcgaag catggctctg aagcggtgtg gccattctat tacgccggca
     1201 cgatgggcct ggtgcagcgc gacggcatca accggttgcg caacgtgatg cgctattccc
     1261 gccagaaaat gacgatctgc acgtcattgc ccgagatcgg ctggcaagcc ggcgtcggcg
     1321 ccagccgagg tgtcgatccg agggaaatgg cgcagtccga cctgatcgtc gtgtggggcg
     1381 gcaatcccgt cgccacccag gtcaacgtca tgacgcacgt atcgcgggcg cggaaggaac
     1441 gcggcgcgaa gcttgtggtg atcgatccat acaagacagc cacggctgcc gtggccgacc
     1501 tgcatctggc gccgcggccg ggaacggatg ccgccttggc ctgcgcggtg atgcacgtcg
     1561 ccttccgcga tggttacgcg gatcatgact acatgcgcaa ctacaccgat tgcccagacg
     1621 cgctggaggc gcatctgcgg gattgcggcc ccggctgggc atcggcgatc acgggcatac
     1681 cgacggagcg gatcgaagcc ttcgcgacgc tttacggtca aacgaaacgc agcttcatcc
     1741 gtgcgggcta cgggttcgcg cgcagccgca acggctgtgc cgcgatgcat gccgtgagtt
     1801 gcctgcccgc cgtcacgggt gcgtggcaac acgaaggcgg cggcgccctg tggtccaatc
     1861 gcggcatgta ccactggaac aagaccctga tcgagggttt ggacgcacaa gatccgtcgc
     1921 ttcgcatcct ggacatgagc cgtatcggca gcgtgctgac cggtgaccgg actgaattgg
     1981 gcgatggccc ccaggttcat tcgatgatca tccagaacca gaatccgctg accgtgtgcc
     2041 ccgacagcaa tcgggtgcgg cgcggctttg gccgcagtga actgttcgtc gcgacgcacg
     2101 aacagttctt gaccgaaacg gtgcgttggt ccgacatcgt gcttccggcg acgatgttca
     2161 tggaacatga cgatatctac caggctggcg gccacagcca tattcaaatc ggcccgaaac
     2221 tgatagcgcc gccgggcgag tgccgctcca accacgaggt gatgcagggg ttggcatcgc
     2281 gtctgggcgc gaagcaccgt ggcttcgata tgaccgcgat ggagctgatt gacgcgactt
     2341 tgcgcgcgtc cggctatccg gatgcgaaga cggtgctgga gcggcgctgg atcgacgcca
     2401 tgccgccatt ccgggaggca catttcctcg atggctttcc aacgaaagat cgccggttcc
     2461 atttcgcgcc cgactggaaa gcgctcggcg acaagcacgc agtgatgccg ggtttgccgg
     2521 atcagatggc gaatatcgag gcggccgggc cggaggcgcc gttccgcctg gtaaccgcgc
     2581 cggcgcgcag tttcctcaac tccacctttt cggagatggc gtcgggccgg aagcgggaag
     2641 gccgtccgac ggtgctgatg catccggacg atgcttctcg cctgggactg atcgagggat
     2701 cgaaggtccg gcttgggaac gtccggggag aggtggtcct ccatgcccgt atctcggacg
     2761 gtcagcagcc gggcgtcctg gtgtcggaaa gcatctggcc gagcgagtgt tttgaaggcg
     2821 ggatcgggat caatgcgttg accagcgacg atcccggtcc cccctatggt ggcgcggtgt
     2881 atcacgactc cgcggtctgg atgcgagccg aagccgtcca ggccgcactg gcggcggagt
     2941 aacaatgggc gcgtgcgacg ccttgcagct aacaaataag gaaacagtcg atgagcggtt
     3001 caaacttcat ggatgactgg gcaccgcgtg tgctcagcgt gctacgcatc gtggcgggtc
     3061 ttcagtttct cgag
//
LOCUS       DASWQN010001139         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_111398, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001139 DASWQN010000000
VERSION     DASWQN010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111398"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..972)
                     /locus_tag="VGC82_19490"
     CDS             complement(274..972)
                     /locus_tag="VGC82_19490"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEY0425511.1"
                     /translation="MENLRLERIAIRIDDERAVSGLLHVPDRATACFVLAHGAGAGME
                     HAFMAATAAGLGQRGIATLRYQFRFMEAGLKRPDGPRTAHAAVRAAVAEAGRRVPALP
                     LFAGGKSFGGRMTSQAQAETALPRIGGLIFLGFPLHAARKPADDRARHLSDVHIPMLF
                     LQGTRDLLAEPSLLIQVAAHLGLLATLHLVADADHSFRVPARSGRTGEQVLGEILDRI
                     VAWIEQIALTVNPL"
     gene            complement(1086..>1626)
                     /locus_tag="VGC82_19495"
     CDS             complement(1086..>1626)
                     /locus_tag="VGC82_19495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0425512.1"
                     /translation="SRYIEAAVEGILIGCIYLPNGNPQPGPKFTYKLAWFERLKAHAA
                     ELLATGLPVVLAGDYNVVPTDDDIYPSKSWTKDALLHPASRTAFRQLLEQGWTDAIRA
                     RHPTGPVYTFWDYKYDRWARDAGLRLDHLLLSPEVAERLADAGVDRDVRGEAGASDHA
                     PAWAMLGRRRRGRSRASAA"
BASE COUNT          335 a          536 c          477 g          278 t
ORIGIN      
        1 gtcatttgct tcgccatcaa atgagccagc gactgcggtc tcgccaaatg tcatttccgc
       61 ggggagccaa ggtagactaa cggctaagca ttgccgtgca ggcctgtccc aagaccatag
      121 aagttgctat gtaattatac tgggtcagta tcatcaacac acccacgacg cgggagttaa
      181 ccaaccgagg ctccgttccc acaaccttaa gttgagttag ttgatgcctc tcaaagttgc
      241 gcacccacgt ctcctatttg aagccaacgg cggtcatagc ggattaactg tcagggcgat
      301 ctgctcgatc cacgccacta tcctgtcaag aatctcgcca agaacctgct cgccggtgcg
      361 tccactgcga gcgggaacgc gaaacgaatg gtcggcatcg gcaaccaggt gcagtgtcgc
      421 gagcaaaccc aggtgggcgg ctacctggat cagcaacgat ggctccgcca acaagtcgcg
      481 cgttccctgc agaaacagca ttggaatatg cacatccgac aaatgccgcg cgcggtcatc
      541 cgcgggcttc ctggcggcgt gcaggggaaa gccgagaaaa ataagcccgc cgattcgggg
      601 caaagcggtc tcggcctgcg cctgcgaggt catgcgtccg ccgaacgact tgccaccagc
      661 gaaaagtgga agcgccggaa cccgccgacc cgcctcggca accgcggccc gcaccgccgc
      721 atgggcggtc cggggcccat cgggtcgttt cagaccggct tccatgaagc ggaattggta
      781 gcgtagcgtg gcaatgccac gctgtcccag ccccgccgcc gtcgccgcca tgaacgcgtg
      841 ctccatgccc gctccggcac cgtgcgccag aacgaaacaa gccgtggcgc gatccggcac
      901 atgcagaagc cctgagaccg cacgctcatc gtcgatacga atggcgatcc gttccagccg
      961 aagattttcc atcgcgaggc gcctgcttca atgatcgacc cgtcccatct ggactgtgtt
     1021 cagaaggaac cgcatcagcg gcgccgtcat gtgacgcgca gcctcagtca tgtgaacgcg
     1081 cagcctcagg cagcggaagc ccgtgatcta ccgcgccggc ggcggccaag catcgcccag
     1141 gccggtgcat ggtcgctggc tccggcctct cccctcacat cccggtccac cccggcgtcg
     1201 gccaggcgct ccgctacctc gggactcaac agcagatgat ccaggcgcaa gccggcatcg
     1261 cgcgcccaac gatcgtattt gtagtcccag aacgtataga ccggaccggt gggatgacga
     1321 gcgcggatgg catcggtcca gccctgttcc agaagctgac gaaatgccgt ccggcttgcc
     1381 gggtgcagca gcgcgtcctt cgtccaggac ttcgaggggt aaatgtcgtc gtccgtcgga
     1441 acgacattgt agtcgccggc caggacgacc ggcaggcccg tcgccagcaa ctcggccgca
     1501 tgcgccttca ggcgctcgaa ccaagccagc ttgtaggtga acttcggccc cggctgagga
     1561 ttcccgttcg gtaaatatat gcagccgatc aggatgcctt cgacggcggc ctcgatataa
     1621 cggctc
//
LOCUS       DASWQN010001140         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2222960, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001140 DASWQN010000000
VERSION     DASWQN010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222960"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /locus_tag="VGC82_19500"
     CDS             complement(<1..1620)
                     /locus_tag="VGC82_19500"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425513.1"
                     /translation="MRLPAALVPLRNSTFRQLWIASVIGWLGTWLQNTGAGWLMTSLA
                     PEPLIVAMVQAATIMPVFLLALPGGALADIVDRRVFLLGTAIWTILAASLLAILTFCH
                     VMTAGWLLALTFAIGIGNALNYPAWSAIVPELVPREDMVQAIALNGIGFNLTRAIGPA
                     IAGFLILLGGSSLAFSMYAVSILAVIGALFVWRRERRFTGLPREHFVSAMRAGMRFVR
                     NTRAIQAAMIRTACYSIPAAAPWALLPLFVRRDLGLGPGMYGVILGMMGIGGVTSGML
                     LPLVRGKLSRGATVVGCSVLSCVGMAVVGMSDHWVPASFGMLLFGLGWTSAFATIQAA
                     AQLVCPPWVRARALSIYQLAQNGALTVGSFGWGWLGGQIGIPHTFLAASIVGVVLMIA
                     ARFFSIEAVVRAPSSVSERIATLPEAPAPEFASLLRNARGRVMEMAYYRVGPLQRVAF
                     LRLMNELRHVRRRAGAVFWQVYEDVAHPEKWVEVWSMESWTEHLREAIRLSEDDKRLL
                     AAVAAFQHEVDRPSRYLAVDPQEYLNAHPSDP"
     gene            1957..>2386
                     /locus_tag="VGC82_19505"
     CDS             1957..>2386
                     /locus_tag="VGC82_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEY0425514.1"
                     /translation="MRVVPVEKQAYREAMARLGAAVNVITTDGPGGRAGFTASAVTSV
                     TDSPPTLLVCANRTNDSYPAMRENGVLCVNTLTPAQEGLSPVFAGMTEHKMNERFDGY
                     AWHTLETGAPVLDQAVVVFDCRINQVIEVGTHDVFFCSVEA"
BASE COUNT          470 a          782 c          790 g          344 t
ORIGIN      
        1 cggatcggag ggatgggcgt tgagatattc ctgcgggtcc accgccaggt agcgggaggg
       61 ccgatcgacc tcatgctgga acgctgcaac cgcggccagc aagcgcttgt cgtcctccga
      121 cagccgtatc gcttcccgca ggtgttcggt ccagctttcc atcgaccaga cctccaccca
      181 tttttccgga tgggcgacat cctcatacac ctgccagaac accgcccccg cccggcggcg
      241 gacatggcgc agttcgttca tcagcctcag gaaggcaacc cgttgcagcg gcccgacgcg
      301 gtaataggcc atctccatga ccctgccgcg ggcgttgcgc agcagcgacg cgaactcggg
      361 agccggcgct tccggcaagg tcgcgatccg ttcgctgacg gaagacggcg cgcgaacgac
      421 ggcctcgatg ctgaagaagc gggcggcgat catcaacacc acgccgacaa tggaagcggc
      481 caggaaggtg tgcgggatgc cgatctggcc accaagccaa ccccagccga aggacccaac
      541 ggtgagtgcg ccgttctgcg ccagctgata gattgacagc gcgcgggcgc gcacccatgg
      601 cgggcacacg agctgcgccg ctgcctggat ggtggcgaag gccgacgtcc agcccagccc
      661 gaacagcagc atgccaaacg aggcgggaac ccaatgatcg gacatcccga cgaccgccat
      721 gccgacacag gacagcaccg aacacccgac gacggtcgcg ccgcgcgaca gcttgccgcg
      781 caccaacggc agcaacatgc ccgaggtaac gccgccgatc cccatcatcc ccaggatgac
      841 gccatacatc cccggaccaa gccccaggtc gcgacggacg aacaatggca gcaatgccca
      901 tggtgccgcc gcgggtatcg agtagcaggc ggtccggatc atcgccgcct ggatcgcccg
      961 cgtgttgcgg acgaaccgca tgccggcccg catggccgat acgaaatgct cgcgcggcag
     1021 gccggtgaac cggcgctccc gccgccaaac gaacagcgcg ccgatcaccg ccaggatcga
     1081 taccgcatac atcgagaacg ccaggctgga gccgcccagc aggatcagga agccggcgat
     1141 cgccggaccg atcgcgcggg tcaggttgaa gccgatgccg ttcagcgcga tcgcctgcac
     1201 catgtcctcg cgcgggacca gttccgggac gatcgcgctc caggctgggt agttcagggc
     1261 gttgccgatg ccgatcgcga aggtcagtgc cagcagccag cccgcggtca taacgtggca
     1321 gaacgtcagg atcgccagca ggcttgccgc caggatcgtc cagatcgccg tccccagcag
     1381 gaacacccgg cgatcgacga tgtcggccag cgcgccaccg ggcagtgcca gcaggaaaac
     1441 cggcatgatc gtcgccgcct gaaccatggc cacgatcagc ggttcgggtg ccagcgaggt
     1501 catcaaccag cccgcgccgg tgttttgcag ccaggtgccc agccagccga tgacgctggc
     1561 gatccaaagc tgacggaagg tgctgttgcg cagcggcacc agggccgcgg ggagacgcat
     1621 cagccctggg ctcgcatggt cggatcggga aggcgcgttg tatccatgaa ccggggtctc
     1681 atgatctcat ctcaaatcgt ttcgctgaca ttgccagggg ggtcgggtcg atctccagca
     1741 taatccgaca cgcacccgaa tgcgcgggat cgtggtgcaa caactcggga aagcgcgtga
     1801 atgttcctcg aacgggatca gccggggccg cagggacggc gctgcgaaac caggcagtaa
     1861 gaacaaggca acggcgtggg gtcgttttga ccgtgccagc cggtcggctg gcctccggcg
     1921 ttgacctgct gcatagtcgt cgccggacaa cggaggatgc gtgtcgtgcc cgtggagaag
     1981 caagcctacc gtgaggcgat ggcccgtctg ggtgccgcgg tgaatgtgat caccacggac
     2041 gggcctggcg gacgcgccgg attcaccgcc agcgcggtca ccagcgtaac cgattcgccg
     2101 ccgaccttgc tggtgtgcgc gaaccggacc aacgactcgt atccagcgat gcgggaaaac
     2161 ggcgtgttgt gcgtgaacac gctgacgccg gcgcaggagg gattgtctcc ggttttcgcc
     2221 gggatgaccg agcacaaaat gaacgagcgg ttcgacggct atgcgtggca cacgttggag
     2281 accggcgcgc cagtgctgga tcaggccgtg gtggtgttcg actgccggat caatcaagtg
     2341 atcgaggtcg gcacccacga cgtgttcttc tgcagcgtcg aggcgg
//
LOCUS       DASWQN010001141         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1694420, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001141 DASWQN010000000
VERSION     DASWQN010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694420"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2338)
                     /locus_tag="VGC82_19510"
     CDS             complement(<1..2338)
                     /locus_tag="VGC82_19510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0425515.1"
                     /translation="MPPHLSGTEPEVVSLARAALTEGLAALAAQDMVSALRWLERAHR
                     LVPHDLNATLALASACLESAPARASSLFADIVGKHDVRQAWLGLAAARLRLDEPEAAT
                     KAMAAVFSRHAFLPDAAALADRIGGHGGWCALRSDGGLEIRGGTAVRVTLDGKSMRGK
                     KLPPDWARGRVIEIRRNDAHLLGSPIRIDAIRRLAGCVEVHGSGIRGWAWHPNDPDTA
                     PVLTVGTACGGVRQTIVALDESITVLDIGPLGRPRSFSLTNAELSGTAGLIHVRGPDG
                     NDLAGSPLDPSADGAAQVATALMIGQAYPAQPQGRNLIGSVRGPLRADAPAPVRPVGA
                     DGQQRAVTIVVPVRDGGPVVLSCLASVLASVPKRARVMVIDDGSSDPELIQALDDLVR
                     LRKITLLRQPRTQGFPASANAGIRAANGRDVVLLNSDTLVPSDWLQRLSAAAYSARDV
                     GTATPLSNDASILSYPGPAGTNPRPDQAATNRLDRLANRANGAATVDIPVGVGFCLYL
                     RRDCLNAVGVFRADVFAQGYGEENDLCLRAQRLGWRNVAATGLFVGHVGGTSFGGSAI
                     HLRRRNSHIVEQLHPGYQTLIDDFLAADPLHESRRRIDLLSWKRRGRKWRQAAILVTH
                     NDGGGVEQLLARSVQLHVEAGRRPIVLRPAETATGQPAIVVHDGLADDLPNLAFAMPN
                     ERPALLRLLRFAKPDRIEAHHLAEYPPAIYDLIARLGLPYDVHVHDYAWVCPRVSLVA
                     RDRYCGEPDVPDCDACVADNGHFLKEDISVAALRQRSGR"
     gene            complement(2342..>3184)
                     /locus_tag="VGC82_19515"
     CDS             complement(2342..>3184)
                     /locus_tag="VGC82_19515"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0425516.1"
                     /translation="GATRALAMLYLTGAGVPRDQEESARLFSIAAQAGDATAHFDLGN
                     LLLKGHGDEQDQIRTRSWFEQAAASGDLIAAFNFGVCLAEGVGIERDDRKAAEWLRRA
                     ADGVVNAQYWYGRMLVEGRGVEANPEEGRVWITKAANVGMIEAEVMLADMMLSGRGGS
                     KDHPAALALFQKAAGKGHVGATFAVGAMFGGGHDIPWDRVAAQRWFRAAAERGHPYAQ
                     MMLGRYLARKLAGEQNIEEARRWTERAVAQGLQEAKVDLAALPPAPSADASSEKNAIR
                     AAGA"
BASE COUNT          565 a         1059 c         1049 g          511 t
ORIGIN      
        1 accggcccga ccgctggcgg agtgcggcga cgctgatatc ttctttcaag aaatggccgt
       61 tgtcggcgac gcaggcgtcg caatccggaa catccggttc accgcaatag cgatcccgcg
      121 ccaccagcga gacacgcggg cagacccatg catagtcgtg gacatgcacg tcgtagggaa
      181 gcccgagccg ggcgataagg tcgtagatgg cgggcggata ttccgcgaga tgatgcgcct
      241 cgatacggtc gggcttggcg aagcgaagca gacgaagcag ggccggacgt tcgttcggca
      301 tggcgaaggc cagattcgga agatcatcgg ccagcccgtc gtgcaccacg atcgccggct
      361 gtccggtcgc tgtttcggct gggcgcagca cgatggggcg gcgtccggct tcgacatgca
      421 actggacgga gcgcgccaga agctgctcca ccccgccgcc gtcgttgtgc gtgacgagga
      481 tcgcggcttg acgccacttt cgtccgcgtc gtttccagga caacaggtcg atgcgccggc
      541 gcgattcgtg caacgggtcg gctgccagga aatcgtcgat caacgtctgg tagccggggt
      601 ggagttgctc gacgatgtgg ctattgcggc ggcgcaaatg gatggcgctg ccaccgaagc
      661 tggttccgcc gacgtgtccg acgaacaaac cggtcgcggc cacgtttcgc caacccagcc
      721 gttgggctcg caggcacagg tcattctcct cgccgtagcc ctgggcaaat acgtcggcgc
      781 ggaacacgcc gacggcgttc aggcagtcgc ggcggaggta gagacaaaag ccgacgccta
      841 ccgggatatc gactgttgcc gccccgttgg cccgatttgc cagacgatcc agccggttgg
      901 ttgccgcctg atccggccgt ggattggtgc ccgccggacc gggatagctg agaatgctgg
      961 cgtcgttcga cagcggtgtg gcggtgccga cgtcgcgggc ggaataggcc gcggcgctca
     1021 atcgctgcaa ccaatccgag ggcaccagcg tgtcgctgtt cagcagcacc acatcgcgtc
     1081 cattcgcggc gcggataccg gcgttggcgc tggcgggaaa cccctgcgtg cgaggttggc
     1141 gcagcagagt gatctttcgc agacgaacca ggtcatccag agcctggatc aattcgggat
     1201 cggacgagcc atcgtcgatg accatgaccc gcgcccgctt cggcaccgat gccaggacgc
     1261 tggcaaggca ggacagaacg accggcccgc catctcgcac cggaacgacg atcgtcactg
     1321 cccgctgttg gccgtcggca ccgacaggtc gcacaggagc gggagcatcg gcacggaggg
     1381 gaccgcggac cgaaccgata agatttcgcc cctgcggttg cgccggatag gcctgcccga
     1441 tcatcaaagc cgtcgcgact tgagcggccc cgtcggccga gggatcgagt ggactacccg
     1501 ccaggtcgtt tccatccggc ccgcgcacat ggatcaggcc cgcggtgccc gaaagttccg
     1561 cgttggtgag gctgaaggag cgtgggcggc caagcggacc gatatcgagg accgtgatcg
     1621 attcgtcgag cgcgacaatc gtttgccgca cgccgccgca tgcagtgccg acggtcagta
     1681 cgggtgcggt gtcaggatcg ttcggatgcc aggcccatcc gcgaataccg ctgccgtgga
     1741 cttccacaca gccggccagg cggcggatcg catcgatcct gatcgggcta cccagcagat
     1801 gtgcgtcgtt gcggcgaatt tcgatgacgc ggccacgcgc ccagtccggg ggcaactttt
     1861 tgccgcgcat cgacttgccg tccagggtca cccgaacggc ggtgccgcca cgaatttcca
     1921 ggccgccgtc ggaccgcaaa gcgcaccaac cgccgtgacc accgatccgg tcggccagcg
     1981 ccgccgcatc cggcaagaac gcatggcgcg agaaaaccgc tgccatcgct ttggtggccg
     2041 cttccggttc gtccagacga agccgagcgg ccgcgaggcc aagccacgcc tgcctgacat
     2101 catgtttccc gacgatgtcc gcaaacagcg acgacgcgcg ggccggggcg ctttccaggc
     2161 aggcgctggc cagggccaat gtcgcgttca ggtcgtgtgg gaccagtcga tgcgcccgct
     2221 ccagccagcg cagtgccgaa accatgtcct gcgcagcgag cgccgcaagg ccttcagtta
     2281 atgcggcacg ggcgagtgaa acgacctccg gctctgtccc gctaaggtgc ggtggcacga
     2341 atcacgcgcc ggccgcgcgg atcgcgttct tttcggacga tgcatccgca ctaggtgcgg
     2401 gtggcaatgc cgccagatcg accttggctt cctgcaagcc ctgggcgacc gcgcgttcgg
     2461 tccagcgcct ggcttcctcg atattctgct cgccggccaa cttccgagcc agataacgcc
     2521 ccagcatcat ctgggcatag gggtggccgc gttcggccgc cgctcggaac cagcgctggg
     2581 ccgcgacccg gtcccacggg atgtcatgac caccgccgaa catggcgcca accgcgaacg
     2641 tggcaccgac gtgacctttg ccggcggcct tctgaaacaa tgccagcgcg gccgggtgat
     2701 ccttcgagcc accgcgtccg gacagcatca tgtccgccag cattacctcg gcctcaatca
     2761 tgccgacatt ggcggccttg gtaatccaga cgcggccttc ctccgggttg gcttcgacgc
     2821 cgcggccttc gaccagcatg cggccatacc aatactgggc attgacaacg ccgtcggcgg
     2881 cacggcgaag ccactcagcc gccttacggt cgtcgcgctc gatgccgacc ccttccgcga
     2941 ggcagacgcc aaaattgaac gcggcaatca gatcgcccga ggccgcagcc tgctcgaacc
     3001 aggaccgggt gcggatctga tcctgttcat cgccgtgccc tttcagcagc agattgccaa
     3061 gatcgaaatg ggcggtggca tcgccggcct gggccgcgat gctgaacagc cgggcggatt
     3121 cctcctggtc acgcgggaca cctgcgcccg tcagatacag catggccagg gcgcgggtgg
     3181 cacc
//
LOCUS       DASWQN010001142         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_111753, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001142 DASWQN010000000
VERSION     DASWQN010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111753"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..410)
                     /locus_tag="VGC82_19520"
                     /pseudo
     CDS             complement(150..410)
                     /locus_tag="VGC82_19520"
                     /inference="COORDINATES: protein motif:HMM:NF018959.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
     gene            538..870
                     /locus_tag="VGC82_19525"
     CDS             538..870
                     /locus_tag="VGC82_19525"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0425517.1"
                     /translation="MTPLRLQMIEDMRMAGLATGTQAVYIDGVRCLAAHYRRAPDQLS
                     EDEVRAYLLGLRERGLALGTFKTNHGGIQFLYRRTLDRDWPLFGGKKDPSPEAAAPAG
                     CPVGRANP"
     gene            797..1381
                     /locus_tag="VGC82_19530"
     CDS             797..1381
                     /locus_tag="VGC82_19530"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0425518.1"
                     /translation="MGGKRIRPPKQRRLPVALSDAQIRKLLGHVRSPTHKTCLVAMYA
                     CGLRIGEAVTLEVGAIDSANLLLRIIGKGNKQRLVPLPPSLLDELRSLWRTHRHPRWL
                     FPNRDGANSLNPQVLGRTFRAAARMAAITQPVTPHSLRHSYATRLLEHGVDPRVVQIL
                     RGHANIATTAIYTHLTEPTRVSLRGVLDRLMSGL"
     gene            1397..>2338
                     /locus_tag="VGC82_19535"
     CDS             1397..>2338
                     /locus_tag="VGC82_19535"
                     /inference="COORDINATES: protein motif:HMM:NF016847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0425519.1"
                     /translation="MIEVADVFRRFAPDYLAAHGASMLPSHQRAITDILACRTEALGG
                     HLWRCDQCHAERFSYHSCKNRSCPKCHTEQTERWLQARQAEMLPSHYFHVTFTVPDEL
                     REVMRANQRDGYGILMKAAVEAIIELARDPHYVGGTVGVLAGLHTWTQQLAFHPHVHC
                     LVTGGGVSDDGRLWHPARNAFLVPTKALAKLVRGKLRAAFATRRPDLVLPAAVWSKPW
                     VVHCTAWGKGEPAVLDYLARYVFRVAITNTRIVGLDDQTVTIRHKHRTSNRWRTIRIP
                     GHEFMRRFLQHVLPKGLHKVRYFGLWRHTKREQAAHAR"
BASE COUNT          392 a          831 c          698 g          417 t
ORIGIN      
        1 ctcgggcgtc tttgcctcgg cagcgatctt cttgggttgg ttctgttgcg cttgcgccaa
       61 agccaggatt ggattgccat acgcgacacc gctcgacgcc gcgccaccgc tatacagcac
      121 gctgaggtca aggccggtga tcgtaacgct catggggagc cggatagacc tgcggtgatg
      181 tcctcgttca ccgcgatcag gaatgcgaaa tcaggtgccg gttcccgcat ttcgcgctgc
      241 aacgcctgtc ccaccgaaac gatcgacgcc cgcagcggcg ctggaagcgc attatccggg
      301 tcacgcagca agtcgccgat cataatccac agccggctgt tgtcggccaa tgccttggtc
      361 tggttcagcg ccgtgccgcc ccgggctgcg cgcaaggcgc cggtgacgcg gcggaaaacg
      421 tccgttggac tgaacggcta cgccgtaggg aagagggcgt cgcgaggcag acgggttggc
      481 ggtattttcg ggtggccctg ggtttgtcct gggcctgcgt ctcgaaagga aaccgttatg
      541 acaccgttgc gcctgcaaat gattgaagac atgaggatgg ccgggttggc gactggcacc
      601 caggccgtct acatcgacgg agtgcgctgc ctcgccgccc attaccggcg agcgcccgac
      661 cagctgagcg aggacgaggt gcgcgcctac ttgctcggac tgcgtgaacg gggtttggcg
      721 ctgggaacgt tcaagaccaa ccacggcggt atccagttcc tttaccgccg cacccttgac
      781 cgagactggc cgttgtttgg ggggaaaaag gatccgtccc ccgaagcagc ggcgcctgcc
      841 ggttgccctg tcggacgcgc aaatccgtaa gcttcttggc catgtcagga gcccgaccca
      901 caagacctgc cttgtcgcca tgtacgcctg cggtctgcgc atcggcgagg ccgtgaccct
      961 ggaagtaggt gcgatcgaca gcgccaacct gcttttgcgc atcatcggca agggcaacaa
     1021 gcagcggctg gtgccgttgc cgccatcgct tctcgatgag ctgcgcagct tgtggcgaac
     1081 ccatcgccac ccgcgctggc tgtttcccaa ccgcgacggc gccaactcgc tcaatccgca
     1141 ggtgctgggc cgcaccttca gggccgccgc tcgcatggcg gcgatcaccc aaccggtcac
     1201 gccgcattca ttgcgccata gctatgcgac gcggctgctc gagcatggcg tcgacccccg
     1261 ggttgtgcag atcctgcgcg gccacgccaa catcgccacc accgccatct acacccacct
     1321 gaccgaaccg acccgcgtct cgcttcgtgg cgttctcgac cgattgatga gcggcctctg
     1381 acgcggcgcc cccgccgtga tcgaggtcgc ggatgttttc cgccgcttcg cgcccgacta
     1441 cctcgcggcc catggcgcgt cgatgctgcc ctcgcatcag cgcgccatca ccgacatcct
     1501 ggcctgccgc accgaagccc tcggcggaca cctttggcgc tgcgaccagt gccacgccga
     1561 gcgcttctcc tatcattcct gcaagaaccg gagctgcccc aagtgccaca ccgagcagac
     1621 cgaacgctgg ctgcaggccc gccaagccga gatgctgccc agccactact tccatgtcac
     1681 cttcaccgtg cccgacgaac tccgtgaggt gatgcgcgcc aaccagcgtg acggctacgg
     1741 catcctgatg aaggccgctg tcgaagcgat catcgagctt gcccgtgacc cgcactatgt
     1801 cggcggcacc gtcggcgttc ttgccggtct gcatacctgg acgcagcaac tcgccttcca
     1861 tccccatgtc cattgcctgg ttaccggtgg cggcgtttcc gatgatggcc gcctatggca
     1921 tccggcccgc aacgcctttc tcgtacccac caaggcgctt gccaagctcg tgcgtggcaa
     1981 gctcagggcc gcgtttgcga cacgccgccc tgatctggtc ctgcccgcgg ccgtttggtc
     2041 caagccctgg gtcgtccact gcacggcctg gggaaagggc gaaccggctg tgctcgacta
     2101 cctcgcccgc tacgtgttcc gcgtcgccat caccaacacc cgcatcgtcg gcctcgacga
     2161 ccagaccgtg accatccgcc acaagcaccg cacgtccaac cgatggcgga ccatccgcat
     2221 ccccggccac gagttcatgc gccggttcct gcagcatgtc ctgcccaaag gcctgcacaa
     2281 ggtccgctac ttcggcttgt ggcgccacac caagcgcgag caggccgccc acgcccgc
//
LOCUS       DASWQN010001143         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_243911, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001143 DASWQN010000000
VERSION     DASWQN010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243911"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..802
                     /gene="pcaH"
                     /locus_tag="VGC82_19540"
     CDS             95..802
                     /gene="pcaH"
                     /locus_tag="VGC82_19540"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEY0425520.1"
                     /translation="MTTGIAPFRSVAPGSQPANDTPSYGSTHLRHPTKPLLRIPQTVT
                     ETTGPSFSPVHFPAMADLSVTNGKQAQGERIIVRGRVVDENDRPVPHTMIEIWQANAG
                     GRYDHPGDQHDAPMDPNFHGGGRVFSDADGWYQFTSIKPGAYPWRNHFNAWRPNHIHY
                     SLFGNGFAQRIITQMYFPGDPLLALDPVYLSVPDQAARERLIARFDIDVTAPEWALGY
                     RFDFVLRGRNATPTEQA"
     gene            804..1325
                     /gene="pcaG"
                     /locus_tag="VGC82_19545"
     CDS             804..1325
                     /gene="pcaG"
                     /locus_tag="VGC82_19545"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEY0425521.1"
                     /translation="MPVATASQTIGPYWHLIEDPTWADLTRFGAKGEKIVLTGTVFDG
                     AGDTVADAAIEIWQTSPEASDTFPAFGRCRTDGQGSFRFTTLKPGPVAGLGNVLQAPH
                     VAISIFARGLLRAVTTRLYFQDEPLNETDPVLSLVEDEPRRRTLIARRISSDTWRLDI
                     RLQGDEETVFMEI"
     gene            1377..>1760
                     /locus_tag="VGC82_19550"
     CDS             1377..>1760
                     /locus_tag="VGC82_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY0425522.1"
                     /translation="MTIAPLPKSIATVSISGTLAEKLEAAAAIGFDGVEIFENDLLTF
                     DGSPKDVRYLAESLGLAITCFQPFRDFEAMPEPQRARNMDRAERKFDVMQQLGAELLL
                     VCSNVSPAVIDDDARAAADLAEMAER"
BASE COUNT          333 a          583 c          531 g          313 t
ORIGIN      
        1 cctcgggctg caaaaccgtt cgatattgag gggtctgaaa tcggcgcatc gcctggcgac
       61 ccgggcgata gcattggtgg gagcagaaga cagcatgaca acgggaatcg caccgtttcg
      121 ttcggtcgcg cctggcagcc agccggcgaa cgacacgccg tcctatggca gcacgcactt
      181 gcggcatccg acaaagccct tgttgcgcat tccccagacc gtgaccgaaa ccacggggcc
      241 cagcttcagc ccagtgcatt tccccgccat ggccgacctg tccgtgacca acggcaagca
      301 ggcgcaaggg gagcggatca tcgtccgcgg ccgtgtggtg gatgagaacg accgtcccgt
      361 cccgcacaca atgatcgaga tctggcaggc caacgcgggc ggccgttacg accatcccgg
      421 cgaccagcac gatgcgccga tggacccgaa tttccatggc ggcggccgcg tgttttccga
      481 cgccgacggt tggtatcagt ttacctccat caagccgggc gcctacccgt ggcgaaacca
      541 cttcaacgcc tggcggccga accacattca ttattcgctg tttggcaacg gcttcgccca
      601 acggatcatc acccagatgt atttccccgg cgatccgctg ctggccctgg atccggttta
      661 cctgtcggtg cccgatcagg cagcacgcga gcgcctgatc gcgcgcttcg acattgacgt
      721 caccgccccc gagtgggcgc tgggataccg cttcgacttt gtcttgcgtg gccgcaatgc
      781 cacaccgacc gagcaggcgt gacatgccgg tcgcgaccgc aagccagacg atcgggccgt
      841 actggcacct gatcgaggat cctacctggg ccgacctgac ccggttcggg gccaagggcg
      901 aaaagatagt gcttaccggc acggtattcg acggcgccgg cgacaccgtc gctgacgcgg
      961 cgatcgagat ttggcagacc agcccagagg cctccgacac attcccggca ttcggccgct
     1021 gccgcaccga cggccagggc tcattccgct tcaccacgct gaaacccggc cctgtcgctg
     1081 gacttggcaa tgtgctgcaa gcgccgcatg tggcgatttc gatcttcgcc cgcgggttgc
     1141 tgcgggcggt aacgacccgg ctctatttcc aggacgaacc gctgaacgag accgatccgg
     1201 tcctgtccct tgttgaagac gaacctcgcc gtcgcacgct tatcgcgcgg aggatctcat
     1261 ccgacacctg gcggctggac atccggctac agggtgacga ggaaaccgta ttcatggaaa
     1321 tctgacagac aggttccttt ccgatccggc gcggacaccg aggggatagc gcggccatga
     1381 ccatcgctcc actgccgaaa tccatcgcga ccgtctccat cagcggcacg ctcgcggaga
     1441 aacttgaagc cgccgccgcg atcggtttcg atggcgtgga aatatttgaa aacgatctgc
     1501 tgaccttcga cggttccccg aaggacgtcc gctacctggc ggaaagtctt ggcctcgcca
     1561 ttacctgttt ccagccgttc cgcgactttg aagcgatgcc ggagccgcag cgggcgcgca
     1621 acatggaccg cgccgaacgc aagttcgatg tcatgcagca acttggcgcg gagctgctgc
     1681 tggtgtgcag caacgtgtct cctgctgtca tcgacgacga tgcccgcgct gccgctgacc
     1741 tggcggaaat ggcggaacgt
//
LOCUS       DASWQN010001144         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1035034, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001144 DASWQN010000000
VERSION     DASWQN010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035034"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VGC82_19555"
     CDS             complement(<1..1043)
                     /locus_tag="VGC82_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY0425523.1"
                     /translation="MKLNIALAARRHGAAPGPVSFFEFWPGWVFYTPVVIHWLLLGLR
                     YGDFSLPTAANPRITTGGLCGESKLSILDQVGPSAQPLVAPACGVLARPDGAAAAEAA
                     MEAFGLNYPVVAKPDIGCNGTGVRLLQDHDALVRYLQAFPRGETVVLQHYVADPNEAG
                     IFYVRHPHEPTGRITSVTVKQPPVVIGNGRSTLRGLIMADERAKLVPHLYLDRLADKL
                     DVIPAKGERVRLVFVGNHCKGSLFRDGRHLVTPALTAAIDRLARSIPEFYFGRIDIRY
                     ASTARLRRGTGFRVIEINGAGSEATHIWDPRTRLLDAWRTQFFHYGAAFRIGAANRRR
                     GFQPSGVRAMYRD"
     gene            complement(1040..1621)
                     /locus_tag="VGC82_19560"
     CDS             complement(1040..1621)
                     /locus_tag="VGC82_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY0425524.1"
                     /translation="MLASSIKSLLVLAGHSAWFQAGAIILGTFILEDAATVMAALQVQ
                     EGALPMGLALVSLYTGIVLGDLGLYGLGWLSARMPWVARRLPPHRQETVRAWISGHIF
                     KVVLVSRFLPGLRLPTYTTCGFVGADLRQFAVAAIAATACWTSLLFAISLKLGQFLMD
                     HLGAWRWAGAAGFVAFIIVAGRAAAAGVQAARR"
     gene            1860..2726
                     /locus_tag="VGC82_19565"
     CDS             1860..2726
                     /locus_tag="VGC82_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY0425525.1"
                     /translation="MDAAFAIGAAINMAFVLAEALFGLMANSVALIADAAHNLGDVLS
                     LLLAWAAAWLTRRPPTARRTYGWGRSSILAALVNATVLLIGVGAIGIEAVRRLMQPEP
                     IASGIVMAVAAVGIVVNGGTALLFMRERHTDLNIRAQFQHLAGDALVSVAVVAAALAI
                     RLTGLTWLDPVVSLGVAWVIVLGTWSTLRESLDLSLDAVPARLAQQDVSDWLGALPGV
                     LEVHDLHIWGLSTTETALTVHLVCEPESAERGLHQLAAALRERFQIGHATIQIETEVD
                     AALCRLRPPGTV"
BASE COUNT          520 a          919 c          875 g          425 t
ORIGIN      
        1 cagtcgcggt acatcgccct gacgccggac ggctggaagc cccgacgccg gttggcggca
       61 ccgatccgga acgccgcacc gtaatggaag aactgcgtcc gccatgcatc cagaagccgg
      121 gtccgcgggt cccagatatg cgtcgcctcc gatccggcgc cattgatctc gatgacgcga
      181 aagccggtcc cacgccgcaa ccgagcggtc gaggcatagc ggatatcgat gcggccaaaa
      241 tagaactccg ggatcgaccg ggccaatcgg tcgatcgccg ccgtcaaggc cggtgtcacc
      301 agatgtcgtc cgtcgcgaaa caacgagccc ttgcaatggt tgccgacgaa gaccagccgt
      361 acacgctccc ccttcgccgg aatcacatcg agcttgtccg ccaaacgatc caaatacaaa
      421 tgcggcacca gcttcgcccg ctcatccgcc atgatcagcc cccgcaacgt cgagcgtccg
      481 ttgccgatca ccaccggcgg ctgcttgacc gttaccgaag tgatccgccc ggtcggctca
      541 tgcggatggc ggacgtagaa gatgccggct tcattgggat cggcgacata atgctgcaga
      601 acgaccgttt cgccgcgggg aaacgcttgc aaatagcgca ccagcgcgtc gtggtcctgg
      661 agcagcctga ccccggtgcc attgcagccg atgtcgggtt tcgccacgac ggggtagttc
      721 agcccgaacg cctccatcgc ggcttcggcg gccgccgcgc cgtccggtcg ggccagcacc
      781 ccacaggccg gcgcaaccaa cggctgcgcc gaaggcccga cctgatccag gatcgacagc
      841 ttcgactcgc cgcacaggcc gccggtggtg atccggggat tggcggccgt gggaaggcta
      901 aaatcgccgt accgcaggcc gagcagcagc cagtggatca ccaccggcgt gtagaacacc
      961 cagcccggcc agaattcgaa aaaagacact gggcccggtg ccgcgccatg ccggcgagca
     1021 gcgagtgcga tgttgagctt catcggcgcg ccgcctgtac gcccgccgct gccgctcgcc
     1081 cggcgacgat gatgaaagcg acgaagcccg ccgcgccagc ccagcgccag gcgcccaaat
     1141 gatccatcag gaactgcccc agtttgagcg atatcgcgaa cagcaggctg gtccaacagg
     1201 cggtggcagc gatcgccgcc acggcgaact gccgcagatc ggcgccaacg aagccgcagg
     1261 tggtataggt cggcaaccgc agtcccggca ggaaccggct gaccagcacg accttgaaga
     1321 tatgtccgga aatccacgcc cggacggttt cctgccggtg cggcggaagc cgccgcgcga
     1381 cccagggcat ccgcgccgac agccagccga ggccgtaaag cccgagatca cccagcacga
     1441 tgccggtgta caaggaaacc agcgcgagcc ccatcggcag cgcgccctcc tgcacctgca
     1501 gcgcggccat gacggtcgcc gcgtcctcca ggatgaaggt gccgaggatg atcgcgccgg
     1561 cctgaaacca cgccgagtga ccggccagca ccaacagtga ctttatagaa ctggccagca
     1621 tggttgggac ggcagccttt ggtgagagaa cacagcaatc ggcaaatggt ctccggaacc
     1681 ggctgacctc atcacctatc cgtcattact ttcccacaac tatagagtga ttgcctgtac
     1741 ggaccgcgca cggcaagatc ggcggcatgg cagcgcataa ccctgaacat catgaacatg
     1801 ggcatcatga acatggggcc caccatagcc atggtgggca tggccacgcc ccggcccgca
     1861 tggacgcggc gttcgcgatc ggtgccgcga tcaacatggc gttcgtgctg gccgaggcgc
     1921 tgttcgggct gatggccaac tccgtggcac tgatcgccga cgcagcgcac aatcttggcg
     1981 acgtgctttc gctgttgctc gcctgggccg ccgcatggct gacgcggcga ccgccaacgg
     2041 cgcgccggac ctacggctgg ggtcgaagtt ccatcctcgc ggcgttggtg aacgcgacgg
     2101 tgctgttgat cggcgtcggc gccatcggca tcgaggcggt aaggcgcctc atgcagcccg
     2161 aaccaatcgc ctccggcatc gtgatggcgg tcgctgccgt cggcatcgtg gtgaacggcg
     2221 gcaccgcgct gctgttcatg cgcgagcggc acaccgacct gaacatccgc gcgcaatttc
     2281 aacatttggc gggcgatgcg ctggtttcgg tcgctgtcgt tgccgccgcg ctggcgatcc
     2341 ggttgaccgg cctaacctgg ctagacccgg tcgtcagcct gggcgtcgcc tgggtcatcg
     2401 ttctcggaac ctggagcacg ctacgcgaat cgctcgacct atcgctcgac gcggtacccg
     2461 ctcggttggc gcagcaggac gtgagcgact ggttgggcgc cctgcccggc gtgctggagg
     2521 tccacgacct gcatatctgg ggactgagca cgacggaaac cgcactgacc gtgcatctgg
     2581 tttgtgaacc tgaatcggcg gagcgaggtc tgcatcaact ggccgcggcg ctgcgggagc
     2641 ggttccagat cggccacgcg accattcaga tcgagacgga agtggatgcg gcattgtgcc
     2701 ggttgcgtcc gccgggaacc gtttaggccc aattggaca
//
LOCUS       DASWQN010001145         3227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2619996, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001145 DASWQN010000000
VERSION     DASWQN010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619996"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VGC82_19570"
     CDS             <1..766
                     /locus_tag="VGC82_19570"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0425526.1"
                     /translation="IWAFGYTGPLKLAPGFDPLLQCYGAVVTLTRRPDDPPTFCAPAI
                     NDTATGMWCVIGALAALKQRDRTGVGCVVDASLFESAVAWVQGPLNAYNVTSVVPKRH
                     GAASATLAPYQIFETADRPICIAAGNDRLFVKLARAMGHPEWCSDPLLSDGRKRAANR
                     HAVVEAMQPVLLSKSRQDWMGIIGEAGVPCAPVNDIAELAESEQLQAMDLMRTLPGSE
                     LKIVGLPITFDRVRPSPRGDSPRLGEHNNEVFGDLG"
     gene            1019..1402
                     /locus_tag="VGC82_19575"
     CDS             1019..1402
                     /locus_tag="VGC82_19575"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY0425527.1"
                     /translation="MAIAQGTVESCAAKGYAVSAVVVDRAGETVLAIRGDNASPHTME
                     NARRKAYTAMSFKLSTTEYAKRFADNNPVVRQQVTLPGVIAIPGGLPIKAGDEVIGGI
                     GASGSPGVDEPCVQAGLDKVADQLK"
     gene            complement(1426..2823)
                     /gene="gltX"
                     /locus_tag="VGC82_19580"
     CDS             complement(1426..2823)
                     /gene="gltX"
                     /locus_tag="VGC82_19580"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY0425528.1"
                     /translation="MTVRTRFAPSPTGPLHIGGVRTALFNYLYSRHCGGEFLLRIEDT
                     DRERSTDEATRGIIESLDWLGLERDGEIVFQSTRMERHAAVARSLLAAGHAYHCYCTP
                     EELAAERELARLEKRIWRYDGRWRDRDPSEAPSGVNPVIRLKTQREGETVLEDLVQGP
                     VRVANAEMDDMIILRSDGTPTYNHSVVVDDHDMGITHVIRGDDHLTNTFRQLQVYRAM
                     EWELPRFAHIPLIHGPDGTKLSKRHGAQFALEFRDDGFLPEAVSNYLLRLGWSHGDLE
                     IVAREEAIRLFDIVDVNRGASRMDYNKLLNLNGVYIRQADDDRLTKDVLERLSSRSDL
                     SIGDDAAARIRTLMPALKERAKTLAELANSASFLVRSVPLPVDPKAQAILTTDARAVL
                     RQVGAALAETDFSVAGIDAALRAFAERSGLKLGQVAQPLRAALTGTTVSPGIDATLAA
                     LGRQEALARIAAAAG"
     gene            2867..>3227
                     /locus_tag="VGC82_19585"
     CDS             2867..>3227
                     /locus_tag="VGC82_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425529.1"
                     /translation="MSSLHRMRPLAAIKAALDSWLDLEQERLAVWLPVFMAAGVLGYY
                     ALLAEPPLWVGPTIGVPAVALAVLLPNNRWLLAPFAAAALGFAAAQTATARAPPIEAD
                     LPAHATVVTGTIRAVEAL"
BASE COUNT          558 a         1025 c         1034 g          610 t
ORIGIN      
        1 gatctgggcg ttcggctaca cggggccatt gaagctggct ccgggcttcg atccgttact
       61 tcaatgttat ggtgcggtag tgacgctgac caggcggccg gacgatccgc ctaccttctg
      121 cgcgccggcg atcaacgaca ccgcaacggg gatgtggtgc gtgatcggtg cgctggccgc
      181 gctgaagcag cgggaccgga cgggtgtcgg ttgcgtggtg gacgcgtcgt tgttcgagag
      241 cgcggtggcg tgggtgcagg ggccgttgaa tgcctacaac gtaaccagtg tggtgccgaa
      301 gcggcatggc gcggcaagtg cgactttggc tccttaccag atctttgaga cggcggaccg
      361 gccaatctgt atagcggccg gaaacgaccg gctgttcgtc aagctggcac gggcgatggg
      421 gcatccggag tggtgttcgg atccgctgct gtcggacggg cgcaagcggg cggcaaaccg
      481 gcatgcggtg gttgaggcga tgcaaccggt gttgctgagc aagtcccggc aggactggat
      541 gggcattatc ggcgaggcgg gagtgccttg cgcgccggtg aacgatatcg cggagctggc
      601 ggaaagcgag cagttgcagg caatggatct gatgcggacg ctgccgggaa gcgaactgaa
      661 gatcgtcgga ttgccgatca ccttcgaccg ggttcgaccg tccccgcgcg gcgattcgcc
      721 gaggctcggg gaacataata acgaggtgtt cggggatctg ggctgacggc cgttgccgcc
      781 gtgcttcggc ttgcctctta cgtgcggcgg catggctcca gttccggcac cccaaccttt
      841 tcgactgccg gcaatatgtt atgccaacca cgatgcgcac gcttttgggg gattgatcat
      901 gcatggttcg cagtcggctc gtcggtggcg ttgggcgatt tatgcagcgg ctgccatatg
      961 cccaggcgcg gcccgggcgg agctgctcgt tcatcacgat ttgccgtatg tggccgcttt
     1021 ggcgatcgcg cagggaaccg tggaaagctg cgcggcgaaa ggttatgcgg tctcggctgt
     1081 cgtggtggac cgcgccgggg aaaccgtgct cgcgatccgc ggtgacaatg ccagtcccca
     1141 caccatggaa aatgctcggc gcaaggccta cacggccatg agcttcaagc tttcgaccac
     1201 cgagtatgcc aaacgcttcg ccgacaacaa tccggtggtg cgccagcagg tgacgctgcc
     1261 cggcgtgatc gcgatcccgg gcggcctgcc catcaaggca ggcgacgagg tgatcggcgg
     1321 aattggtgcc tccggctcac cgggcgtgga cgagccgtgc gtacaggcgg gattggataa
     1381 ggtcgcggat cagttgaagt gagatcaagc cgcgcctgaa cggcttcaac ctgcagcagc
     1441 tgcgattcgc gccagcgctt cctgccgccc gagcgccgcc aaggtcgcgt cgattcctgg
     1501 gctgaccgtg gttcccgtca aagccgctcg taaaggctga gcgacctgac caagcttcag
     1561 tccactccgt tccgcaaatg cccgcaaagc ggcatcgatc cccgcgacag agaaatccgt
     1621 ctccgccagc gccgccccga cttgccgcaa caccgcccga gcgtcggtcg tcagaatggc
     1681 ttgcgccttc gggtccaccg gcaacggcac gctccgaacc aggaacgagg cggagttggc
     1741 caactccgcc agcgtcttcg cccgctcctt caaagccggc atcaacgtcc gaatccgagc
     1801 cgccgcgtcg tcaccgatgg acagatccga tcgcgacgac agacgctcca gcacgtcctt
     1861 cgtcaaccgg tcatcatcgg cttgccggat atagactccg ttcagattca gcagcttgtt
     1921 gtagtccatc cgagatgccc cgcggttgac atcgacgatg tcgaacagcc ggatcgcttc
     1981 ttcccgtgcg acgatttcaa gatcgccgtg cgaccagccc aaccgcagca ggtagttgct
     2041 gaccgcctcc ggcaagaacc cgtcatcacg gaattccaat gcaaactgcg ccccgtgacg
     2101 cttggacaac ttcgtcccgt ccgggccgtg gatcagcgga atatgggcga atcggggtaa
     2161 ttcccattcc atcgcccggt aaacctgcaa ttgccggaag gtgttggtca ggtggtcgtc
     2221 gccgcggatc acatgggtga tccccatgtc gtggtcatcc accaccaccg agtgattgta
     2281 agtcggcgtc ccgtcgctgc gcaggatgat catgtcgtcc atttcggcat tggcgacgcg
     2341 caccggacct tgcaccagat cctccagcac cgtttcgcct tcccgctggg ttttcaatcg
     2401 gatgaccggg ttcacgcccg agggtgcctc cgacggatcg cgatcccgcc agcgcccgtc
     2461 ataccgccag atccgctttt ccaggcgcgc caactcacgc tccgcggcca gttcttccgg
     2521 cgtgcaatag cagtgatagg catggcccgc cgccaaaagg ctccgagcca ccgcggcatg
     2581 ccgctccatc cgggtggact gaaacacgat ctcgccatcg cgctccagcc caagccagtc
     2641 caggctctcg atgatgcccc ttgtcgcctc atcggtgctg cgttcgcggt cggtgtcctc
     2701 gatccgcagc aggaattctc caccgcagtg gcggctgtac aggtagttga acagtgcggt
     2761 tcgaaccccg ccgatgtgca gcgggccggt cgggctaggg gcaaagcgag tacgtacggt
     2821 cataccgaca gtttagcatg cttgttcact ctttgtagac acttgcatgt ctagtctgca
     2881 ccgcatgagg ccactcgctg ccatcaaagc cgcgctggac tcctggctcg accttgagca
     2941 ggagcggctg gcggtatggc tgccggtctt catggccgcc ggcgtactgg gttattacgc
     3001 actgctcgcc gaaccgccgc tgtgggttgg accgacgatt ggggtgccgg ccgtcgccct
     3061 ggcggtgttg ttgccgaaca accgatggct acttgccccc ttcgccgccg cagcacttgg
     3121 ttttgccgcc gcccagacgg cgaccgctcg ggcgccgcca atcgaagccg acctccctgc
     3181 ccacgcgacc gtcgtcaccg gcacgatccg ggcagtggag gcgctgc
//
LOCUS       DASWQN010001146         3591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_770353, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001146 DASWQN010000000
VERSION     DASWQN010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770353"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..562)
                     /locus_tag="VGC82_19590"
     CDS             complement(176..562)
                     /locus_tag="VGC82_19590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425530.1"
                     /translation="MAKTSTTTRVPRGAKILTKAFFAAADEIPEPQRAEVVKAALALI
                     RDELKAAREKVAVAKAKAKEKAGKASTSSVHKPASVAKAPAKIQNKVASVQAKGPAKR
                     ASKAAPKSAEKTAPNKSNETTVRSDT"
     gene            complement(939..1181)
                     /locus_tag="VGC82_19595"
     CDS             complement(939..1181)
                     /locus_tag="VGC82_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425531.1"
                     /translation="MAAKERIVYQPYVRGARGAVKPGTPVPCRTAEEALRRAEKAMAS
                     ASVVGAHVVRVMADEEAGDYGDPEYLASLGTIPNVD"
     gene            complement(1547..1828)
                     /locus_tag="VGC82_19600"
     CDS             complement(1547..1828)
                     /locus_tag="VGC82_19600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425532.1"
                     /translation="MRHAGSALDARAILRLYVERVPAVGYEGSCIFHGKQGCTLDRSL
                     RSDLCNAYFCGGLYTYLTGGEAATPTVIIAGEGKKMRTSPVMMPREKPG"
     gene            2404..3162
                     /locus_tag="VGC82_19605"
     CDS             2404..3162
                     /locus_tag="VGC82_19605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0425533.1"
                     /translation="METSGKIAVITGGGSGIGRATALALCADGFSVVLAGRRREMLEE
                     TAALAKAPPPRMLVVPTDVADPASIASLFDTVKATYGRIDLLFNNAGISTRNIPVEDL
                     TIDQWSNVVAVNLTGSFLCAQHAFRMMKSQAPRGGRIINNGSVSAHVPRRNSTPYAAT
                     KHAMTGLTRSLSLDGREYDIACGQIDIGNAATARNEDTARGRLQASGKIEAEARIDVN
                     DVARGVAYMASLSLEANVQFMTVMATKMPYIGRG"
BASE COUNT          707 a         1095 c         1057 g          732 t
ORIGIN      
        1 accgaccgca tccgatcgat cggaaccagg gattgggcaa cggtcaatgg ctagggccgc
       61 ggtataagaa gaagttgtga agggcgatcg gagaactcct ctctctgtct ctcagcccgg
      121 ctggtgcagt gtccggaaca caggatccgg ccaattagat taacttcaga ttgaatcagg
      181 tgtcggaccg gacggtcgtt tcgtttgact tattcggcgc ggtcttttct gccgacttgg
      241 gtgctgcctt gctggcgcgc ttggcgggcc ccttggcctg aacggacgct accttatttt
      301 gaatcttggc aggcgccttg gcaacgctag ccggcttatg gacagaactc gtcgatgcct
      361 tgccggcctt ctccttggct ttcgccttcg caacggccac cttctcccgt gcggccttca
      421 gttcgtcgcg gatgagcgcg agagcggctt tgacgacttc ggcacgttgt ggctccggga
      481 tctcatcagc cgcggcaaag aacgccttgg tcagaatttt tgccccccgt ggcacgcggg
      541 tcgtggtgct tgttttcgcc atatgtttca ttccttatgc aaaccattag cacttggcag
      601 tgccgcaagc cttttgcaag agaacaatgc acggtcggtg attcttgaca tgaagaggca
      661 aggtttaacc gtcacaacaa aagaccggat gttggtccca aagcgtctat acaaaatcac
      721 atgttccatc cgtacttggg taatcatcca ccttcagtct ggcgtgccag cagttctcaa
      781 tgcaattgag cttggtcccg cgagtgatgg ccagaggtgc tgagtctgga atggcacagt
      841 ttgtcacgaa tgattgtttg tcctgtgggt aggatattgc caagcgccgc ggaggcgatt
      901 cgttgcccgc agggacgcag gctccgcccg gacctgcctc agtccacatt ggggatggtg
      961 ccgagggagg ccaaatattc gggatcaccg taatcgccgg cctcctcgtc ggccatgacg
     1021 cgaaccacat gcgccccgac caccgacgcc gaggccatgg ccttctcggc gcgtcggagc
     1081 gcctcctcgg ccgtgcgaca cggcactggt gtgccgggct tcaccgcccc gcgggcacct
     1141 cggacataag gctggtagac gatacgctct ttggcagcca cacagggtct cctggatcag
     1201 agtacagcgc tgatctagac caaggatcgg ataaggggat cacagtatgc gccgcctgga
     1261 caatggagcg ccatcggttg gacttgttag tccacggcgt gacggaccga aaacactacc
     1321 tcgggccttt tgggtatcgt gcccatgagc cactcccgcg gtctggaccg agcgcctgcc
     1381 tccgccttgg ttccggaccg cgatgacaag caccttgctg cccctaaccc caataccgtt
     1441 caattagatc tgtcggtgac cagcagcgat gggccagcag gctggaaagg gccaaccaat
     1501 agatacttac ttggatcaga ctaggcttaa ctgaacggta ttgcccctaa cccggttttt
     1561 ccctcggcat catgactggc gaagtgcgca tctttttgcc ttcgcctgcg atgatcactg
     1621 tcggtgtcgc tgcttcgccg ccagtcagat aggtgtacag cccaccacag aaataggcgt
     1681 tgcacagatc ggatcgcagc gaccgatcca gcgtgcagcc ctgttttccg tggaaaatgc
     1741 acgagccctc gtagcctacc gccggcactc gctcgacata cagccgcagt attgcacgcg
     1801 catctagcgc cgatcctgcg tggcgcacgc gcgttattgt ccgctcatca agaaagccgt
     1861 cgtcatcgcc attcctgcag caccatcctc ggcacaacga acaagcggcg tgagccaccc
     1921 gggcgacaaa gccctcaggt tccggccgta catacgacgc ggtttctgga tctttcactg
     1981 cgtacaatat tctgaccaga tgtttgcgca gccgccgcac gcgctcaggc gatatcggca
     2041 ccagcatctt gtcgttgcga ggcaccatca ctggtagggc gtcatcgtcg gagctatgtc
     2101 ggtctcggtg aggcatgtga gtttccagtt gatctgaccg cagtgcgatc gtccaaacgt
     2161 gaaaaagccg gtgtcaaagg gatcccaccc ggccatccca acgctgtcat cgcggcacat
     2221 ccttcgtcaa ctaagccggc attcgcagcc ggtcagtccg tcggaaattc cctcaagcca
     2281 ttggcgccga tcacaccgca gtcccactgc gtgacaattc acaacggaag cgaacggttg
     2341 ccctctatcg gcatggggcg gaggatgggt gatgcctttg gcttcaccgg gaggtaccgg
     2401 gctatggaaa catcgggaaa gatcgcagtc atcaccggcg gcggcagcgg cattgggcgg
     2461 gcaaccgcac tcgcgctgtg tgcggacgga ttttccgtgg tgctcgctgg gcggcgccgc
     2521 gagatgctcg aggaaacggc tgcgctcgcc aaggcgccgc cgccgcggat gctggtggta
     2581 ccaaccgatg tcgctgaccc ggcatcaatc gcgtcgttgt tcgacaccgt caaggcgacc
     2641 tatggccgga tcgatcttct gttcaacaac gcgggcatca gcacccgcaa catcccggtc
     2701 gaggatctga ccatcgatca atggtccaac gtcgtcgccg tgaatttgac cggctcgttc
     2761 ctctgcgcac agcacgcctt ccgcatgatg aaaagccaag cgccgcgcgg tgggcgcatt
     2821 atcaataacg gctcggtctc agcccatgtg cccagacgaa attcgacacc ttatgccgcg
     2881 accaagcacg caatgaccgg cctcacccgg tcattatcgc ttgatgggcg cgaatacgac
     2941 atcgcctgcg gccagatcga catcggcaat gccgcgaccg cgcgcaacga agacacggca
     3001 cgaggccgct tgcaggccag cggaaagatc gaggcggagg cgcgcatcga cgtaaacgac
     3061 gtcgctcgag gggtcgccta tatggcgagc ctgtcactcg aagcgaacgt tcagttcatg
     3121 accgtgatgg cgacaaagat gccctatatc ggccgcggct gacgctgtcg cgaagacgga
     3181 tcaggggtct accgatgagc cgtcgctggc ctgtcggacg tcagctgcct tatgtgctca
     3241 cgtatccggc agtcgcggtt tgcgagttgc gtcggccgcc tgcaataggc gcgatcagaa
     3301 cgctttcgaa ctccgcgagg cagcgacaat gacacaattg tcgcgaggcg tcggctgctg
     3361 gtgcaactga cgctgaagga cgaccgcgtg tgggtatcaa cgccggtcgc gtagaggccg
     3421 tttccagtca gacaagctgc gcagcgcgtt ggtgatcagc actcgaagcg ctcgccctgc
     3481 ttcgcgagag acgttggcga taggggatag ccgtggcatc ccctgccccg cagtcggcag
     3541 cgccggcgtc gataccggta gccttgcgtc atcccttctt attgccacgg a
//
LOCUS       DASWQN010001147         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1300365, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001147 DASWQN010000000
VERSION     DASWQN010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300365"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VGC82_19610"
     CDS             complement(<1..507)
                     /locus_tag="VGC82_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0425534.1"
                     /translation="MANFENIIVETEGRVGLIRLNRPAALNALCDALVRELGEQLQAF
                     DADKAIGAIVITGSDRAFAAGADIKEMRDRTNPAAMVEDFIGAGWESVLRIKKPVIAA
                     VAGFALGGGCELAMMCDMIFAADTAKFGQPEINLGVIPGAGGTQRLTRAVGKSKAMDM
                     VMTARMMDA"
     gene            complement(868..>1512)
                     /gene="mutM"
                     /locus_tag="VGC82_19615"
     CDS             complement(868..>1512)
                     /gene="mutM"
                     /locus_tag="VGC82_19615"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY0425535.1"
                     /translation="SGWSVLIHLGMSGRMLVEPVGRNDITLHEHIELETDDGWRVGFV
                     DPRRFGSVDLIETDREDSHRLLAGLGPEPLDDAFSVAVLASALIGKKTPIKAALLDQK
                     IVAGLGNIYVCEALFRARISPQRLAHSVPGTRAKRLVPAIKQTLTEAIAAGGSSLRDY
                     VQPDGELGYFQHAWKVYGREGEPCPDCPADVTCDGVRRIVQAGRSTFYCPRHQH"
BASE COUNT          283 a          492 c          443 g          294 t
ORIGIN      
        1 cgcatccatc atgcgggctg tcattaccat gtccatggct ttggatttac ccacggcgcg
       61 ggttagccgc tgcgtgccgc cggcgccggg gatgacgcca aggttgatct ccggctggcc
      121 aaactttgct gtatcggcgg cgaagatcat gtcgcacatc attgccagtt cacagccgcc
      181 gcctaaagcg aaaccggcca ccgccgcgat caccggcttc ttgatgcgca gcacgctttc
      241 ccatccggcg ccgataaaat cctcgaccat ggcggcagga ttggtgcggt cacgcatttc
      301 cttgatgtcg gccccggctg cgaaggcgcg gtcgctcccg gtgatgacga tggcgccgat
      361 cgctttgtcc gcgtcgaacg cctggagctg ctcgcccaat tccctgacca gcgcgtcgca
      421 cagcgcgttc agcgccgccg ggcgattgag ccggatcagc cccacccggc cttcggtttc
      481 gacgatgata ttttcgaaat tcgccatgcg gcacttctcc tggaggcggc gtcttcatcg
      541 ccgacaatca caaccgcatt catcatatca gacgccgcat gttcgaacag ggagagcccg
      601 gaccagcatc cgaaaacttc ggattggacg gagctccggg cgtgatcgcc tgaggttgac
      661 ccggtcggaa ggcttggatc acgcgatcaa acacagggtt ggaccatgat cgaacatcgt
      721 gaagggttca gcctcaaacg atcatgtctg atggagagcg gtggctagac tgttttccgt
      781 ttgcctgggc tcactgaaaa cgatctggct cattgtttta gcgagtatct tcacccgttc
      841 agataatcta tctgaacggg atctgctcta atgctggtgc cgcgggcaat agaacgtgct
      901 gcggccggcc tgcacgatcc ggcgcactcc gtcgcaggtc acatcggcgg ggcagtcggg
      961 acaaggttcg ccctcccggc cgtacacttt ccatgcgtgc tggaaatatc ccagttcccc
     1021 atccggctga acgtagtcac gcagcgaaga tccgccggcg gcgatcgcct cggtcagcgt
     1081 ctgcttgatc gccggcacca ggcgtttggc ccgcgtcccc ggcacactgt gcgccagccg
     1141 ctgaggcgag atgcgggcgc ggaaaagcgc ctcgcacacg tatatattgc ccaggccggc
     1201 gacgatcttc tggtccagca atgcggcctt gatcggcgtc ttcttgccga tcagcgccga
     1261 cgcgagcacc gccaccgaaa atgcgtcatc cagcggttcg ggccccaacc ccgcgagcag
     1321 gcggtgagaa tcttcacgat ccgtctcgat caggtcaacc gacccgaagc gccggggatc
     1381 gacgaaaccg acgcgccaac cgtcatcggt ctccagctcg atgtgctcgt gcaacgtgat
     1441 gtcgttccgc cctacgggtt ccaccaacat gcggccggac atgccaaggt ggatcagcac
     1501 cgaccagccg ct
//
LOCUS       DASWQN010001148         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_904208, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001148 DASWQN010000000
VERSION     DASWQN010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904208"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2021)
                     /locus_tag="VGC82_19620"
     CDS             complement(<1..>2021)
                     /locus_tag="VGC82_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0425536.1"
                     /translation="EGLLFLPPKQLSALMDQTIDAQPFLGQLVADPSARGLFAALSLL
                     GMGVTQGDADLSPYLAPIKGFHDSMANALAGHAKPLSWEALLGGGLSEMAGPYRFVLA
                     QPKQDFGSLQPGGAATKAMRDIIADLPFVKSGDAHVRITGQVALADEEFATVAEGAVT
                     GLLGSLVLITIWLFLAVHSWRLIVPILMTLLLGLMLTVLFAAVAVGTLNLVSVGFGVL
                     FVGIAVDFAIQFSVRYREFRFETGDPAAAMTETARRVGVQILIAALATAAGFLAFVPT
                     AFSGVAELGLIAGAGMIMAFICTLGFLPAAITLFRPRGEAAEVGFAFAARVDPVIVRG
                     KWLILAGFGVLAVAALAVSPRLMFDSDPLDTKNPNTEAMRTLRDLINNPVTNPYTIDV
                     LAPDAAAAQAISPRLEALPSVSGVISINSFVPADQQQKLAVIADANTILAPTLAPHGT
                     AATVTAEQIRMAAKAALGQIDPALVRLPKDHPLASVAEDLRRMQTASDDVLLATNNAL
                     TRFLPSQLGHLRLALGAEPVTLAAIPADFARDWLLPDGRARVQVNPKPAAQTPSGLKA
                     FVQEVSAVAPNAGGSAVTISATADTIVGAFRNAATYAVIAIAVILFGLLRRVLDAALV
                     LAPLLLSALLTLLVAVLLPLPLNFANIIALPLLLGVGVSFNIYFVMNWR"
BASE COUNT          403 a          659 c          665 g          294 t
ORIGIN      
        1 cgcgccagtt catcacgaaa tagatgttga acgacacacc gacgcccagc agcaacggca
       61 gcgcgatgat gttggcgaag ttgagcggca atggcagcag cacggcgacc agcagcgtca
      121 gcagggccga cagcagcagc ggcgccaaca ccagtgcggc gtccagtacg cggcgaagca
      181 aaccgaacag gatcaccgcg atcgcgatca ccgcgtaggt cgcggcattg cggaacgccc
      241 ccacgatggt gtcggcagtg gcggaaatgg tcaccgccga cccgcccgca ttcggggcga
      301 ccgccgatac ttcctgcacg aacgccttca acccgcttgg tgtttgcgcc gcgggctttg
      361 gattgacctg cacccgcgcc cggccgtcag gcaacagcca atccctggcg aaatccgccg
      421 gaatggcagc caaggttaca ggttccgcgc ccagggccag ccgcagatgg ccgagctggc
      481 tcggcagaaa gcgggtcagc gcgttgttgg tcgccaacag cacgtcgtcg gaagcggtct
      541 gcatccggcg cagatcctcg gccaccgatg ccagggggtg atcctttggc agcctcacca
      601 aggcaggatc gatctgtccc aaggccgcct tcgccgccat ccggatttgc tctgcggtca
      661 ccgtcgcggc ggtcccatgc ggggccagcg ttggagccag gatggtgttg gcgtccgcga
      721 tcaccgccag cttctgctgc tgatcggccg gaacgaagct attgatgctg atgacccccg
      781 agacgctcgg cagggcttcc agcctggggc tgatcgcctg tgcggcagcc gcgtccggcg
      841 caagcacgtc gatcgtgtag ggattggtga ccgggttgtt gatcaggtcg cgcagcgtcc
      901 gcatcgcctc ggtgttgggg ttcttggtgt ccagcgggtc ggaatcgaac atcagccgcg
      961 gcgagaccgc cagcgcggcg accgccagca cgccgaaccc ggcgaggatc agccattttc
     1021 cccgcacgat gaccggatcg acccgcgcgg cgaaggcgaa gccgacctcg gccgcttcgc
     1081 cacgtgggcg gaacagcgtg atcgcggccg gcaggaaacc aagcgtgcag ataaaggcca
     1141 tgatcatccc ggccccggca atcaggccaa gttcggcgac gccggaaaac gcggttggca
     1201 cgaacgccag gaagccggcg gccgtggcca gagcggcgat caggatttgc acaccgaccc
     1261 ggcgcgcggt ttcggtcatc gccgccgcgg gatcgccggt ttcgaagcgg aattcgcggt
     1321 agcggaccga gaactgaata gcgaaatcga cggcaatgcc gacgaacagc acgccgaatc
     1381 cgaccgacac cagattgagc gtgccgaccg cgaccgcggc gaacagcacg gtcagcatca
     1441 gaccaagcaa cagggtcatc aggatcggca cgatcagccg ccacgaatgc accgccagga
     1501 agagccaaat cgtgatcagc accagactgc ccagaaggcc cgtgacagcg ccctcggcaa
     1561 cggtggcgaa ttcctcgtcg gccagcgcca cctggccggt gatgcggaca tgcgcgtcgc
     1621 cggatttgac gaacggcagg tcggcgatga tgtcgcgcat cgccttggtc gcggcaccgc
     1681 ccggttgcag tgagccgaag tcctgcttgg gttgcgccag cacgaagcga taaggacctg
     1741 ccatctcact caggccgccg cccagcaggg cttcccacga tagcggcttc gcgtggccgg
     1801 caagcgcatt ggccatcgaa tcatgaaagc ccttgatcgg ggccagatac ggcgacagat
     1861 ccgcatcgcc ctgggtcacg cccatgccga gcagtgacaa tgctgcgaac aggccgcgcg
     1921 cggaaggatc ggcgaccagt tggcccagga aaggttgggc atcgatagtc tgatccatca
     1981 atgccgagag ttgcttcggc ggcaggaaga gcaatccttc c
//
LOCUS       DASWQN010001149         4566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_507614, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001149 DASWQN010000000
VERSION     DASWQN010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507614"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..342)
                     /locus_tag="VGC82_19625"
     CDS             complement(100..342)
                     /locus_tag="VGC82_19625"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0425537.1"
                     /translation="MPLAEIAVILRRALRDHDLEISPDTRFEDFADWDAMDLISVVAE
                     IECRFELQFEVVDIDRLTTVGDLLCMVSAKQALAAA"
     gene            complement(514..1107)
                     /locus_tag="VGC82_19630"
     CDS             complement(514..1107)
                     /locus_tag="VGC82_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY0425538.1"
                     /translation="MATSRPKSDTAAMDGFLTGQILIAMPAMADPRFQQSVIYLCAHT
                     PEGAMGLVLNRPIVKPTFDDLLKQLHVEPLPPVRQIRLCAGGPVENARGFVLHTNDWT
                     GEGSLKVDDAMALTASLDVLKVIAEGGGPRECVLALGYAGWGPGQLDQEIQQNAWLSV
                     QADETLLFDGGHDTKWRRAFAKLRVDPMLLSGTAGHA"
     gene            1197..1613
                     /locus_tag="VGC82_19635"
     CDS             1197..1613
                     /locus_tag="VGC82_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY0425539.1"
                     /translation="MPTEAPEDNTMPNAAESPLATYIAHLKRGELRYQYSPSAGRAVF
                     FPRVIAPGTGAADLEWRKSSGFGTVHATTVVHPQQGESYNVCLVDVDEGFRIMSRVED
                     IDPTGVKIGMRVKFRVHTPTGDDDPYPVFTPVGADQ"
     gene            1610..2764
                     /locus_tag="VGC82_19640"
     CDS             1610..2764
                     /locus_tag="VGC82_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEY0425540.1"
                     /translation="MTGVTRGSVAIVGAAESELGAVAALMSPIDLMAQGIYRALEDAG
                     LTLKDVDGVFCATTQARTSAMSLVEYLGLPNAYTDSTIVGGSSFEVHVAHAHAALEAG
                     LCSVAVVAYGSTQRTVGRRAASSREFNPYETPFKPFLPASAYAMVASRHMHEYGTTRE
                     QMAAVAVAAREWALLNPVAWEKKPLTIADVLNARPISDPFTVRDICLVTDGGGAIVLV
                     KAGRAKSMKQKPVYVLGSGQSITHASISAMPVLTHTGAIESGRQAYRAAGLTASDIDV
                     VALYDAFTINTILFLEDLGFCPKGEGGRFVEGGRIAPNGALAVNTNGGGLSYCHPGMY
                     GLFLLIEAVRQLRGECGPRQVANAELALVHGNGGVLSAQATTILGGPSTI"
     gene            complement(2834..3412)
                     /locus_tag="VGC82_19645"
     CDS             complement(2834..3412)
                     /locus_tag="VGC82_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEY0425541.1"
                     /translation="MYRYYMNRVYLAGPDVFLPDAVDWIGRKKAICAGFDLIGVSPLD
                     EPTAVPAEWGHLPEWRRIALRNEAHIRDCAAVIANLTPFRGPSADVGTVYEVGFARGL
                     GLKVFGYTTVAAAFLDRTLSAAGGGRLDQDGSWWDAEGLLVEQFGLFDNLMIEGGIVE
                     SGGTLVRQDQDRWYDLSVFEQCVQTAAAVLNA"
     gene            complement(3487..>4566)
                     /locus_tag="VGC82_19650"
     CDS             complement(3487..>4566)
                     /locus_tag="VGC82_19650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEY0425542.1"
                     /translation="GEEQQERQRASALLQTIIETAPGLIYAKGLDGGLLVANQPLLDL
                     IGKPWDRVKGRTDREYFDNVAEAEAIMANDRRIMARGAAEEVEEFMGSAKGIRRLWLS
                     TKTPMRDADRTVIGLVGVSVEITERKRSEDRLQTLVYELNHRVKNTLATVQAITAQTL
                     RRADPILREGLEARLMALAAAHDVLTRESWQGADLHEVLRTALAPFGGFAGNRFNISG
                     PGLRLQPRAALSLSIALHELATNALKYGALAVSTGKVALHWDIEDAADPRFHLIWAET
                     GAGPIMEPEHRGFGSRLIERVVARDLRAKTTLEFTAKGVVCTIDAPTSEIVLAAEAME
                     FPLVGRMAAFRAGEASPHGVSKTVQ"
BASE COUNT          905 a         1425 c         1406 g          830 t
ORIGIN      
        1 aagccatcca gcgccaagcc ggctgaacag gacgcaaccg cacagcagca gcggcaggaa
       61 acccaacaga aaaggaaagc cgctgaacgt cactgcgtct cacgccgcgg caagcgcctg
      121 tttggccgaa accatgcaaa gaaggtcgcc gacagtggtt agccggtcga tgtcaacgac
      181 ctcgaactgc aattcaaacc ggcactcgat ctccgcgacg acggagatca ggtccatcgc
      241 atcccagtcc gcaaaatcct cgaaacgcgt atccggtgag atctccagat cgtgatcgcg
      301 aagcgcccta cgcaggatga cggctatttc ggctaaaggc atacccacga cctacttccg
      361 cattccgtgc atcagcacgc aggagtggtt tagtgcggga aaactaccga agcgttaacg
      421 cgcaaccggt tgattaggat tgtcgccagc taccgccttg tattgtcgcc agcaaccggc
      481 caaccggttt ttgctccgcc ggcgactgat cggtcaagcg tgtccggcag tgcccgacaa
      541 cagcatcggg tccacccgca gtttcgcgaa agcgcgccgc cacttggtat cgtggccgcc
      601 atcgaacagc aaagtttcgt cggcctgaac cgacaaccag gcattctgct ggatctcctg
      661 gtcgagctgc cccggccccc agccagcata acccagcgcg aggacgcatt cgcgcgggcc
      721 accgccctcg gcaattacct tcaacacgtc caggctggcg gtcagcgcca tggcgtcatc
      781 gactttcagg ctgccctctc cggtccagtc attggtgtgg aggacgaatc cgcgggcgtt
      841 ttccaccgga ccgccagcac aaagcctgat ttggcggacc ggcggcaacg gctcgacatg
      901 caactgcttc aacaggtcgt caaacgtcgg tttgacgatt gggcggttca acaccaagcc
      961 catcgcaccc tccggcgtgt gggcacagag atagatcaca ctttgctgga agcgcgggtc
     1021 agccatggca ggcatggcga tcagtatctg gccagtcagg aagccgtcca tcgcagccgt
     1081 gtcggatttg ggacgagaag tagccatggg acaaatctgg tcccgacctg agcgcggtgc
     1141 aagcgtcatg gtatcggtta ggtgctggtt tgtctgtgat cggatgcatt gctagcgtgc
     1201 cgaccgaagc gccggaggac aacacgatgc caaatgcggc cgaatcgcca cttgcgacct
     1261 atatcgcgca tctgaagcga ggcgaactga ggtaccagta cagcccgtcg gccggccgag
     1321 ccgtgttctt tccgcgagtc atcgcacccg gaaccggggc agcggacttg gaatggcgaa
     1381 aaagttccgg ctttggcacg gtgcatgcga ccaccgtggt acatccacaa cagggcgaga
     1441 gttataatgt ttgcctggtg gatgtggacg aaggcttccg catcatgagc cgggtcgagg
     1501 atatcgatcc gaccggggtg aaaataggaa tgcgagtaaa attccgcgtg cacaccccga
     1561 cgggcgacga cgatccttat ccggtgttca cgcccgttgg agccgatcaa tgaccggcgt
     1621 gacccgcggc agcgttgcca tcgtcggcgc cgccgaatcc gagttgggag cggtcgcggc
     1681 gctgatgagc ccgatcgacc tgatggcgca agggatctac cgcgcgctgg aagatgcggg
     1741 gctgacgctg aaagatgtcg atggggtgtt ctgtgcgacc acccaggcac gaacctcggc
     1801 gatgtcgctg gtcgagtacc tcggtctgcc gaacgcttat accgacagca cgatcgtcgg
     1861 cgggtcctcg ttcgaagtgc atgtcgcgca tgcccacgcg gcgctggaag caggattatg
     1921 ttccgtcgca gtcgttgcct atggcagtac ccagcgcacg gtcggtcggc gcgccgccag
     1981 ttcccgcgag ttcaacccgt acgagacgcc tttcaagccg ttcctgccgg caagtgctta
     2041 tgcgatggtg gccagccgcc acatgcacga atacggcacc acccgcgagc aaatggccgc
     2101 cgtggccgtg gccgctcggg aatgggcgct gctgaatcca gtggcgtggg agaaaaagcc
     2161 gctgaccatc gccgacgtgc tgaatgcccg cccgatcagc gatccattca ccgtgcggga
     2221 tatctgcctg gtgacggatg gcggcggggc gatcgtgcta gtgaaggccg gccgcgcgaa
     2281 aagcatgaag cagaagccgg tttatgtgct gggaagcggg cagtcgatca cgcacgcctc
     2341 catcagcgcg atgccggtgc tgacgcatac cggtgcgatc gaatccggcc ggcaagcata
     2401 ccgggcggcg ggtctgacgg catcggatat cgacgtggtg gcgctgtatg atgcctttac
     2461 gatcaacacg atccttttcc tggaggatct gggtttttgc cccaagggcg aaggcgggcg
     2521 ttttgtcgaa ggcggcagga tcgcgccaaa cggggcgctg gccgtcaaca ccaacggtgg
     2581 cgggctgtcc tactgccatc ccggaatgta cgggctgttc ttgttgatag aagccgttcg
     2641 ccaactgcgc ggggagtgcg ggccgcggca ggttgctaat gcggaactcg cgttggttca
     2701 tggcaatggc ggtgtgctgt cggcgcaggc gacgactatt ctgggtggac catcgacgat
     2761 ttaagaccgt gaacacgccg ggcggaacgt ccgaagcgtg cgaaccatgc gattgttgaa
     2821 gtcggaatgc cggtcaggcg ttcagcacgg ctgcggctgt ctgaacgcat tgttcaaaaa
     2881 ccgacaagtc gtaccagcgg tcctggtcct ggcggaccag ggtgccgcct gactccacga
     2941 tcccgccttc gatcatcagg ttgtcgaaca ggccgaactg ctccaccagc aggccttcgg
     3001 catcccacca ggagccgtcc tggtcgagcc ggccgccacc cgccgccgat agcgtgcgat
     3061 cgaggaatgc cgccgccacc gtcgtgtagc cgaacacctt caggccgagg ccgcgggcga
     3121 agccaacctc atagaccgtg ccgacatcgg cgctaggtcc gcgaaacggt gtcaggtttg
     3181 cgatgacggc ggcacagtcg cgaatatgag cttcattccg cagcgcaatc cggcgccatt
     3241 ccggcagatg tccccattcg gccggcaccg cagtgggttc gtcgagggga gagacgccaa
     3301 taaggtcaaa tccggcacag atcgcttttt tccgaccgat ccaatccacg gcatcgggca
     3361 ggaagacatc cggaccggcg aggtacaccc gattcatgta ataacgatac atcaacgaat
     3421 cggattcggc aagtgaaatt gaccatgatg aaggaaaccg gcagccgatc ctggtaacaa
     3481 ctgaggttat tggacggttt tggacacacc gtgcggtgag gcctcacccg cacggaaagc
     3541 cgccatccgt cccacgagcg gaaactccat cgcctcggcg gcgaggacaa tctccgacgt
     3601 cggggcatcg atggtgcata cgacgccttt cgcggtgaac tcgagagtag tcttcgcgcg
     3661 caaatcccga gccaccacac gctcgatcaa gcgcgagcca aagccacgat gctccggttc
     3721 catgattgga ccggcgccgg tttcagccca aatcaggtga aagcggggat cagccgcgtc
     3781 ttcgatatcc caatgcaagg ccacctttcc tgtcgaaacc gctagcgcgc catatttcag
     3841 ggcatttgtg gccagttcgt gtagcgctat ggacaggctc aacgccgcgc gcggctgcag
     3901 gcgcaggccc ggccctgaaa tgttgaagcg attccctgcg aaaccgccaa atggtgccag
     3961 tgccgtgcgg agcacctcgt ggaggtcggc accttgccaa ctctcccgcg tcagcacgtc
     4021 gtgcgcggcc gcgagcgcca tgagccgtgc ctccagccct tcgcgcaaga tcgggtcggc
     4081 ccgccggagg gtttgcgcgg tgatcgcctg caccgtcgcc agggtgtttt tcacgcggtg
     4141 attcagctcg tagaccaacg tctgcaaccg gtcctcgctc cgcttgcgtt cggtgatctc
     4201 gaccgacacg ccgaccaaac cgatcaccgt ccgatccgcg tcgcgcattg gggtcttggt
     4261 cgatagccag agccggcgaa tccccttcgc gcttcccatg aactcctcca cctcctcggc
     4321 cgcaccgcgt gccatgatcc gccggtcatt cgccatgatc gcttcggctt ccgcgacatt
     4381 gtcgaagtat tcgcggtccg ttcggccctt tacccgatcc cagggcttgc cgatcaggtc
     4441 cagcagcggc tggttcgcga cgagcaggcc gccgtccaag cccttggcgt agatcaggcc
     4501 gggcgcggtc tcgataatcg tctgcagcag ggcactggcg cgctgccttt cctgttgttc
     4561 ctcgcc
//
LOCUS       DASWQN010001150         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1959768, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001150 DASWQN010000000
VERSION     DASWQN010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959768"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VGC82_19655"
     CDS             <1..587
                     /locus_tag="VGC82_19655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425543.1"
                     /translation="MPQPPIAPSTPAAHVVDLMTAHGSAAFGARWKGMEAKIVTCPAL
                     ADSMREFQTTYDIDPHAEIIGFDDTAWPEIAATDLGARRGGGMVSFFWFRTTLTIPTR
                     ALDYETNGAKAVLTVNVDDYAEVWVNGELPRAAGRPSPAAIQGFNMPNRIVLRDAVAP
                     GDKFEVAVFAINGPISAAPANFLWFREAKIEFYR"
     gene            complement(602..1477)
                     /locus_tag="VGC82_19660"
     CDS             complement(602..1477)
                     /locus_tag="VGC82_19660"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0425544.1"
                     /translation="MSLTFSIGDLTIHRIIESEGPLFDPLTFFPTLTRELLDENLPWL
                     KPDFVDPVSLQLMLCIQSYIVRTPHHNILIDSCVGNHKERLNYPFWNRLTSDRYEKNL
                     AATGLTVDDIDYVMCTHMHVDHVGWNTRLENGRWVPTFPKAKYVFADRELAFWTDKER
                     DDPGKQPWIVDSVLPIIHAGRQEVVTSRHELSDVVKLIPTPGHTIDHYSVRVGKPGAD
                     AVIGGDMIHSPLQARYPELAMRADYDRAQGGASRRSLFSELCDTPTLLCTGHFPSPST
                     GRLTRWGEGFRYNPV"
     gene            complement(1500..2831)
                     /locus_tag="VGC82_19665"
     CDS             complement(1500..2831)
                     /locus_tag="VGC82_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425545.1"
                     /translation="MVEAIEARTMAKVSRHLIPFVIICYFVAYLDRVNLSFAALEMNK
                     DLNFTSTVYGTGAGAFFISYFLLETPSNLILLKMGARRWIARIMFTWGILSGAMAFVW
                     NDTSFYVIRFLFGAAEAGFFPGILFFLTLYFPAVYRARMVSMFMVAIPVSAVIGAPLS
                     TFILFMDGILGLRGWQWIFISEALPALVLSVVTWFYLTDHPSQAKWLQPEEREWLVKR
                     QEAEVRQREAAHKFSVLQMLRNPRVIALGIVGFGIGYEGYGLVYFLPQIVKQFGLTNM
                     QTGFVSAIPFAVGAVGMVWYGRRSDRVLERRSHTAVTFLIAAIGLVLTAVTPNPYIRL
                     VTLSFASFGIYAVLPVFWSMPTSFLSGAAAAAGVAYINSIANISGFVGNYIMGWLRDT
                     TGSFDSGLLVIAAVCNVAAFTTLCIRHDKELELVPETEDRPPLVGNVANII"
     gene            complement(2918..>3179)
                     /locus_tag="VGC82_19670"
     CDS             complement(2918..>3179)
                     /locus_tag="VGC82_19670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425546.1"
                     /translation="RGRAVAAVTVAVAVARNVRRRIIRSLPVLYFAMSMRNKAGSCKL
                     RLLCLLQLLIHGPSPAFALARADHDGRTARQILDFGRSWQKT"
BASE COUNT          659 a          974 c          976 g          570 t
ORIGIN      
        1 tcatgccgca gccaccgatc gccccttcga cgccggccgc tcatgtcgtc gatctgatga
       61 ccgcgcatgg atcggcggcg ttcggcgcgc gatggaaggg gatggaggcg aagatcgtca
      121 cctgtccggc gctggcggat tcgatgcgtg aatttcaaac gacctacgac atcgatccgc
      181 acgccgagat catcggcttt gacgatacag cgtggccgga gatcgccgcg acggacctgg
      241 gcgccagacg gggcggcggc atggtgtcgt tcttctggtt ccgcacgacc ctgacgatcc
      301 ccacccgtgc cctggattat gaaaccaatg gcgcgaaggc ggtgctgacg gtgaacgtgg
      361 acgactacgc ggaagtctgg gtcaacggag aactgccacg cgccgcgggc cggcccagcc
      421 cggcagcgat ccagggattc aacatgccca atcgcatcgt gctgcgcgac gccgtcgccc
      481 ccggcgataa attcgaagtc gccgtcttcg ccatcaacgg gccgatctcc gctgcgccgg
      541 ccaacttcct ttggttccgc gaggcgaaga tcgagttcta tcgctgaccg gggacgtctg
      601 gtcagaccgg gttgtagcgg aacccttcgc cccaccgcgt caaccttcct gtcgatggcg
      661 acggaaaatg cccggtgcag agcagcgttg gcgtgtcgca cagctcgctg aacaggctgc
      721 gccgcgatgc tccgccctgg gcgcgatcat agtccgcgcg catcgcaagt tcgggatacc
      781 gagcctgaag cggggaatgg atcatgtcgc cgccgattac cgcgtcggcg cccggtttgc
      841 cgacccgcac cgaataatga tcaatcgtgt gacccggcgt cgggatcagt ttgacgacgt
      901 cgctgagttc atgccggctg gtgaccactt cctggcggcc ggcatgaatg atgggtagaa
      961 cagaatcgac gatccacggc tgtttgccgg gatcatcgcg ctccttgtct gtccagaacg
     1021 cgagttcgcg gtcagcgaat acatatttgg ccttggggaa ggtcggcacc cagcgcccgt
     1081 tctccagtct cgtgttccag ccgacatgat cgacatgcat gtgggtgcac atgacgtagt
     1141 cgatgtcatc caccgtcagt ccggtcgcgg cgaggttctt ctcgtaacgg tcggatgtca
     1201 gcctgttcca gaacggatag tttagccgct ccttatggtt accgacgcaa ctatcgatca
     1261 ggatgttgtg atgcggtgtg cgcacgatgt acgactggat gcagagcatc agttgcaggc
     1321 tgacaggatc tacaaaatcc ggcttcagcc agggcaggtt ttcgtccagc agttcccgcg
     1381 tcaacgtcgg aaagaaagtc agcggatcga acagcggccc ctccgactcg atgatccgat
     1441 ggatggtcag gtcgccgatg gaaaacgtga gactcatgat cggtgtcctc ggatgcacat
     1501 cagatgatgt tggcgacgtt gccgaccagc ggtggccggt cctcggtctc gggcaccagc
     1561 tccagttctt tatcgtgacg gatgcacaaa gtggtaaacg ccgccacgtt gcagaccgcc
     1621 gcgatgacca gaagaccgct gtcgaagctg ccggtcgtgt cgcgcagcca gcccatgatg
     1681 tagttgccga caaagccgga gatattggcg atcgagttga tataggcgac gccggccgcc
     1741 gccgccgcac cgctcaggaa cgaagtgggc atcgaccaga acaccggcag caccgcgtag
     1801 atgccgaacg aggcaaagct gagcgtcacc agccggatat acgggttggg ggtcactgcc
     1861 gtcagcacca gcccgattgc cgcgatcagg aacgtcaccg cggtgtggct acgccgctcc
     1921 agcacccggt cggagcgcct gccgtaccac accatcccga ccgcacccac ggcgaacgga
     1981 atggccgaga cgaaaccggt ctgcatgttg gtcagaccga actgcttgac gatttgcggc
     2041 aggaagtaaa ccagcccata gccctcgtat ccgatgccga agccaacgat gccaagcgcg
     2101 atgacccttg gattgcgcag catctgcagg accgaaaact tgtgcgcggc ttcacgctgg
     2161 cgcacctctg cctcctggcg cttgaccagc cattcacgct cctccggctg cagccacttt
     2221 gcctgcgacg gatggtcggt cagatagaac caggtgacaa ccgagagaac caacgccggc
     2281 agcgcctcgc tgatgaaaat ccactgccag ccgcgaaggc cgaggatgcc atccatgaac
     2341 aggatgaatg tcgatagcgg cgcgccgatg accgccgaga ccgggatcgc gaccatgaac
     2401 atgctgacca tgcgggctcg gtaaaccgcg gggaagtaaa gcgtcaggaa gaacaggatg
     2461 ccggggaaaa atccagcctc cgcggcgccg aacaggaacc ggatgacgta gaaactggtg
     2521 tcgttccaga cgaacgccat cgcgccggac aggatgcccc aggtgaacat gatgcgcgcg
     2581 atccagcggc gtgcgcccat cttcagcagg atcaggttgg atggcgtttc cagcaggaag
     2641 tagctgatga aaaacgcccc cgcgccggtg ccgtagaccg tcgaggtgaa gttcaggtcc
     2701 ttgttcatct cgagcgcggc gaagctgagg tttacgcgat cgagataggc gacgaagtag
     2761 cagatgatga cgaacggtat caggtgtcgc gacaccttcg ccatggtacg ggcttcgatt
     2821 gcttcgacca tccctgttcc cacctttggc acgaccgcgc tgttcggcgg ctctggtttg
     2881 acgccaggtt ccaggctgga aggataaacc ggactggtca agtcttctgc caggaacgac
     2941 cgaaatcaag gatctgacgt gccgtcctcc cgtcatggtc cgcgcgcgcc agcgcaaatg
     3001 caggcgacgg tccatggatg agcagttgca gcaagcataa aagccgaagt ttgcaactgc
     3061 ccgctttatt gcgcatgctc atcgcaaaat aaagaacagg gaggctacgt attatgcgac
     3121 gccgcacatt cctcgccacc gccaccgcca ccgttacggc ggccactgcc ctgccccgc
//
LOCUS       DASWQN010001151         1632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1300516, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001151 DASWQN010000000
VERSION     DASWQN010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300516"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(826..1041)
                     /locus_tag="VGC82_19675"
     CDS             complement(826..1041)
                     /locus_tag="VGC82_19675"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0425547.1"
                     /translation="MLCTPVLAQDVRKKLGLSQAAFAKLLGIPVATLRNWEQNRFVME
                     PAAQTLLKLIDREPEAALRALRGPQAA"
     gene            complement(990..1184)
                     /locus_tag="VGC82_19680"
     CDS             complement(990..1184)
                     /locus_tag="VGC82_19680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425548.1"
                     /translation="MTLEDVKAQATARSPEEQAAFQRRMAATTEADIHRQMIEDGEDP
                     QTEPCFAPPSWPRTFARSSA"
     gene            complement(1181..1453)
                     /locus_tag="VGC82_19685"
     CDS             complement(1181..1453)
                     /locus_tag="VGC82_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEY0425549.1"
                     /translation="MVEFEWDLAKSAATEQSRGIGFARAAEIFTSRTVEWADNRHPYG
                     EARVRVVGISAGELLHVVYTRRGTVIRIISARRANKQERAQWLSSA"
BASE COUNT          309 a          502 c          502 g          319 t
ORIGIN      
        1 gtcatccagc gcatcgccca gagtttcccg aagagcgaca tcatcctcga ctagtagcag
       61 gcgggagcgc gatcccatgc gtttctactc cattcgcagt caacataacg cccagccgca
      121 cgttcagcta tccacagagc agagctgcaa gaccgcgttg acgataaagc catgacgctg
      181 gtggtcagcc agaacaatct gcaatataac gaaatagtga taacgtctac gttaaggtgt
      241 cgttattcag tcgtgatgcc gtagccgttg ccgccgggtt caataacgca gctggacagc
      301 agcccagcgc cgaccagcaa cgcgatcacg gtcaagaact tggcgcatga tatctgtcct
      361 cagtcgcgcg gtgagtagcc ataagccggt ggataccgac ggctgtcgtc ccatcgacga
      421 tgttcttccc gccgttgatg ccgagcccaa cgatgatgct cgcgcgcgca ttcacgctcc
      481 catcgacgat gttcccagca acggggaccg catccatccc aataaatttg ctcgacaccc
      541 gacggatgca cgaccgcagg tggaaccgat agaggatgag cggtagccgg cacattcggt
      601 ttgagtaccg gcggccggat aaatacagct gtctgcgctg gaacccaggg cgggaggaat
      661 ggcgcggggc gccggcagcc ttctcgccag ggcctgtgcg actgcaagcg cagcttggcc
      721 ggcccgccgg cgtcgaagcg acagcggctg aatttatgtc ccgtcggcag gatcttgcat
      781 tgggtgcgag ttgatcatgc agcgttggat ccatcacggg aacaattagg cagcctgcgg
      841 tccacgcaga gcgcgcagcg ccgcttccgg ctcacggtcg atcagcttga gcagggtttg
      901 cgcggcgggt tccatgacaa accggttctg ctcccagttg cgcaaggtgg cgaccggaat
      961 gccgagcagc ttggcgaagg cggcctgact caggccgagc ttcttgcgaa cgtcctgggc
     1021 caggacgggg gtgcaaagca aggctcggtc tgcggatcct cgccgtcctc gatcatctgc
     1081 cgatggatgt cggcttcggt ggtcgcggcc atgcggcgct ggaaggccgc ctgttcctcg
     1141 ggcgatcgtg ccgtggcctg cgccttgacg tcttcgagtg tcatgcggat gagagccact
     1201 gggcgcgctc ctgtttgttg gctcggcgag ccgagatgat gcggatcacg gtgcctcgtc
     1261 tggtgtagac cacatgcagc aattcgccgg cgctgatgcc tacgacccgg acgcgggctt
     1321 cgccgtatgg atggcggttg tcggcccact caaccgtccg gctggtgaag atctccgcag
     1381 cccgggcgaa gccgataccc cggctttgct cagtggcggc gctcttcgcc aggtcccatt
     1441 caaactccac catccaacta tacgcgtaac gcgtatggtt cgcaaaggaa tcttagaaat
     1501 catggccgcg cggcggatga tgcgaacgtc cccgccttgc cgtttgtcat gcctatgtcc
     1561 cctgccccac ttaggttcgt cacccaaatg ctgggagcgg ctcgctcgtc ctctcgtcac
     1621 ggcttgaggc ag
//
LOCUS       DASWQN010001152         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_904430, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001152 DASWQN010000000
VERSION     DASWQN010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904430"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..919
                     /locus_tag="VGC82_19690"
     CDS             182..919
                     /locus_tag="VGC82_19690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425550.1"
                     /translation="MSDPNMSGPAMSNPTGSSHTCSPIDLDTGLMLTVADLLIPLFAT
                     GTDDTPMARRTAAGAIEAYRPETQADYVNAARTIAFSMAALSLLGKSASPEMTTQEQM
                     RTFARAIALNRSADQSERTMMQRRRQQQADLHAEQPSEPPALDAEVDDARVQAAIGEA
                     MQEYLAARPPAKAEPAAAVSGSKPTPVKRHEVALPERFQTLSPLEAAIRDSSAVFASR
                     PPRSMPYRSELLQHAALHLVTGHELPG"
     gene            1048..1557
                     /locus_tag="VGC82_19695"
     CDS             1048..1557
                     /locus_tag="VGC82_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425551.1"
                     /translation="MKSLAVVLAPVLLLAACASSQQRTVNLLERRLEARLQPDIAAGR
                     ATVQPMPGAARVTLLDDSRFSTGTMALDDKYPDPRSNVIEGMLDPSLMQVQITDTSNL
                     PEEQRDERVRNVAAYFAANGLGAVLSTADPASPAASPAGLAITIGVICPSNTDSYGYG
                     SGRSTPACY"
     gene            complement(1710..>2376)
                     /locus_tag="VGC82_19700"
     CDS             complement(1710..>2376)
                     /locus_tag="VGC82_19700"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0425552.1"
                     /translation="GAFVVAGDVSDCADAAAAAGNGAFGVHLDVTDVASCDRMAERAI
                     ERFGRIDALVNNAALYGSLRGGRFNQIAEGDWDAAMAVNVKGIWNCCKAVVPGMRQAG
                     GGSIVNIASLAATYGMPFALHYTTSKAAVIGLTRGLARELGRDSIRVNAVAPSAVLTE
                     GTAEFFGDKMDRGLEVVRTGQSIQRTLEPSGLVGTIAWLVGDGSKFVTGQTISVDGGT
                     VMH"
BASE COUNT          460 a          854 c          667 g          395 t
ORIGIN      
        1 ggagcctccg gtcaccgccg aagacccttg cccacaatgg taaaatccag ggcagtgctc
       61 aaggcggttg cattttcaca tgagattttt cttgcctccc cgcaacatgc cgtcgtaaga
      121 acatttcgag aacttgaagc caacctattc aaggccgtcg ctccccatgt ccgatccaaa
      181 tatgtctgac ccaaatatgt ccggcccagc catgtccaac ccaaccggat cgagccacac
      241 ctgttcaccc atcgacctgg ataccggcct gatgttgacg gtcgccgacc tgctcatccc
      301 actcttcgca accggcaccg acgacacccc catggcgcgc cgaacagcag cgggcgccat
      361 cgaggcctat cgtccggaaa cacaggccga ctacgtcaac gccgcccgca cgatcgcctt
      421 ctcgatggcc gctctcagtc tgctgggtaa atccgcatcg ccggaaatga cgacgcagga
      481 gcaaatgcgc acgttcgccc gcgccattgc cctgaaccgc tccgccgatc aaagcgagcg
      541 cacgatgatg cagcgccgcc gccagcaaca ggccgatctg catgctgaac agccatccga
      601 accaccggca cttgatgcgg aagtcgacga cgcccgcgtc caggccgcga tcggagaagc
      661 catgcaggaa tacctggccg cccgcccacc ggccaaggcc gaacccgccg cggcagtttc
      721 cggatccaaa ccaacgccgg tgaagcgaca tgaagtcgca ctgcccgaac gtttccagac
      781 gctcagcccg ctcgaagccg cgatccgcga cagcagcgcg gtgttcgcca gccgtccgcc
      841 gcggtcgatg ccgtacagat ccgaacttct gcagcacgcg gcgttgcatc tcgtcacagg
      901 ccacgaactc cccgggtagc gctcgcggcc gggatacgcc gacagccggc tgcaaaattg
      961 ttgcaaccag tcctgaattc cgaaacactt gcggcgttga acatctgatc gaccacgcgc
     1021 cagcccgcgg caggagaatt ttggaccatg aaatcgctcg ctgtcgtcct cgctccagta
     1081 ttgctgctag ccgcctgcgc ctcgtctcaa cagcgcacgg tcaacctgct ggaacgacgg
     1141 ctggaggcgc gccttcagcc cgacatcgcg gccggccggg caacggtgca gcccatgccc
     1201 ggagccgctc gggttacgct gctcgatgac tcgcggtttt cgactggaac gatggcgctg
     1261 gacgacaaat atcccgatcc caggtccaac gtcatcgaag gcatgctgga ccccagcctg
     1321 atgcaggtgc agatcaccga tacgtccaac ctgccagagg agcagcgaga cgagcgggtg
     1381 cggaacgtag ccgcgtattt cgccgccaat gggctcggcg cggtgttgag caccgccgat
     1441 ccggcttccc ccgcggcgtc acccgcggga ttggcgatca cgatcggcgt gatttgcccg
     1501 agcaacacgg atagctacgg ttacggctcc ggccgctcga ccccggcctg ctattgatag
     1561 cgcccggctg tcgcctccag tccgccaaac gccacgcctg gaccatgatc gtttgaggtt
     1621 gaaccctcca caatgctcga tcatggccta acccatgtct gatcgcgtga ttacgcgttc
     1681 tgacggggtc aacctcaggc gatcgcgctc taatgcatca ccgtcccgcc atcgaccgag
     1741 atggtctggc ctgtcacgaa cttgctgccg tcgccgacca gccaggcgat cgtgccgacc
     1801 aggccggacg gttccagcgt gcgttgaatc gactgaccag tgcggacgac ctccaggcca
     1861 cggtccatct tgtcgccaaa aaactccgcc gttccctcgg ttagcaccgc gctcggcgcc
     1921 accgcattaa cccggatcga gtcccggcca agttcgcggg ccagtccgcg ggtcagcccg
     1981 atcaccgcgg ccttggaggt cgtgtagtgc agcgcgaacg gcatgccata cgtggccgcg
     2041 agggacgcga tgttgacgat ggatccaccg ccggcctgac gcatgcccgg caccacggct
     2101 ttgcagcagt tccagatgcc cttgacgttg acggccatcg ccgcgtccca gtcgccctcg
     2161 gcgatttggt tgaaccgccc gccgcgcagc gaaccataca gcgcggcgtt gttcaccagc
     2221 gcgtcgatcc ggccgaaccg ctcgatcgcc cgttccgcca tccgatcgca ggatgcgaca
     2281 tccgtcacgt ccagatgcac cccaaatgcg ccgtttcctg ccgccgccgc cgcgtcggcg
     2341 cagtcggaaa catcaccggc cacgacgaat gccccc
//
LOCUS       DASWQN010001153         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_244948, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001153 DASWQN010000000
VERSION     DASWQN010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244948"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..491
                     /locus_tag="VGC82_19705"
     CDS             237..491
                     /locus_tag="VGC82_19705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425553.1"
                     /translation="MPYALFSNDAKLSKTYPTEADVWRLAHRSGLVVDAISEEEKAAP
                     RPVLDHDYEIRSCQPDPHEDPAQNKAEAERDARSELRLDS"
     gene            complement(566..805)
                     /locus_tag="VGC82_19710"
     CDS             complement(566..805)
                     /locus_tag="VGC82_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425554.1"
                     /translation="MRAFALALILSAAAVPCLAQTVLKSEPFILAPYEIAFVQDASCP
                     TGKVLKVTGAIRGLHRRKACVTLAAEQASLATATP"
     gene            complement(989..>1591)
                     /locus_tag="VGC82_19715"
     CDS             complement(989..>1591)
                     /locus_tag="VGC82_19715"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0425555.1"
                     /translation="PQVVYNWTGFYIGGHVGGAFAGGNSLLGNDARFLGGVQGGFDYQ
                     FAGNWVVGAEAQYSWLANNNNGVLFPGGTLVTSNNNQLGSVTGRFGYAWGPALLYAKG
                     GYAWRDNANLAVVNAGAPAAFTTGGNHRDGYTVGAGLEYMFAPSWSAKAEYQYYDFGK
                     TQLTAGPADITGRSFRNDEHTVKVGVNYRFGWGGPVAAKY"
BASE COUNT          338 a          487 c          492 g          274 t
ORIGIN      
        1 tggggccatc tgcatctggc ggctgattcc aggccatagc gttttcaagc gaagtggatg
       61 tccggttcgc gtgaagaaaa cgcgtcaaaa caaaaagata gagccccgtt ccgattcaat
      121 cggaacgggg gctctagcgc cgttaacccc aaatgctgca tagcagcaaa gcgaaaggcc
      181 tgtggctgaa accaaaaccc ggggctaagc gttgaccgcc aattccggag ccgacgatgc
      241 cctacgcgct gttttccaac gatgccaagc tgagcaagac ctacccgacc gaggccgacg
      301 tctggcggtt ggcgcatcgg tcagggctcg tggtcgatgc catctcggaa gaggaaaagg
      361 cagcaccgcg cccggtgctg gaccatgatt acgagatcag gtcgtgccag cccgacccgc
      421 atgaagaccc ggcacagaac aaggccgaag ccgagcgcga cgcccggtcc gagttgcgat
      481 tggattcgtg aggcggcgcg gagaagccat cccggctgcc gagtacaaac gccccggccg
      541 ggacgacagt aaatgattca tcttctcagg gcgttgcggt cgccagtgaa gcctgttccg
      601 cagcaagggt gacgcaggcc ttgcgccggt gcagacccct gatcgcaccg gtcaccttga
      661 gcacctttcc ggttggacag gacgcgtcct gcacaaaggc gatttcgtaa ggcgcgagga
      721 tgaacggttc ggatttcagg acggtctggg cgaggcacgg cacggccgct gcggaaagta
      781 tcagcgctaa cgcaaaagca cgcatgttcg tatgtccaac caacccggct gaaccataat
      841 agcgcaacat gacaaaaagt tgcgtgcact gcgaaaatta ttttcgcaca gcatgatcgg
      901 ccaatggccg acgcttcatc cgtcgcagcc caaataaaaa ggccggcttg gcgccggcct
      961 cttgtcgttt gaatacgcgg accgcgtatc agtatttggc ggcgaccggg ccaccccagc
     1021 caaagcggta gttcacgccg accttgacgg tgtgttcgtc gttccgaaag ctccgcccgg
     1081 tgatatcggc gggacccgcc gtgagctggg tcttgccgaa atcatagtac tggtactcgg
     1141 ccttggccga ccagctaggc gcgaacatgt attcgaggcc ggcgccgaca gtatagccgt
     1201 ccctgtggtt gccgcccgtg gtgaaggccg ccggtgcgcc ggcgttgacc acggccaggt
     1261 tggcgttgtc gcgccaggca taaccgccct tggcgtaaag cagcgccggg ccccaggcgt
     1321 agccgaaccg tccggtcacc gaccccagct ggttgttgtt cgaggtgacc agggtgccgc
     1381 ccgggaacag cacgccgttg ttgttattgg cgagccagga atactgcgcc tcggcgccaa
     1441 ctacccagtt gccggcgaat tgatagtcga agccgccctg cacaccgccg aggaagcgcg
     1501 cgtcattgcc caacaggctg ttgccgccgg caaacgcgcc gccgacatgg ccgccgatat
     1561 agaagccggt ccagttataa accacctgcg g
//
LOCUS       DASWQN010001154         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2224410, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001154 DASWQN010000000
VERSION     DASWQN010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2224410"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /gene="xdhB"
                     /locus_tag="VGC82_19720"
     CDS             <1..1641
                     /gene="xdhB"
                     /locus_tag="VGC82_19720"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HEY0425556.1"
                     /translation="SLVAVAASLLAQRTGRPVKHRLDRDDDMILTGKRHHCRIDYDVG
                     FDNDGRIRGISFVQSIGCGYSPDLSGAIADRAMFHADNAYYLDHVHIVSHRCKTHTVS
                     NTAFRGFGGPQGMMGIEHVVDEIARHLHIDPLTVRKRNFYGTHDRNVTPYHMTIEDNI
                     ISEVVAELEREAGYAVRREEVGAFNAANPWIKRGLALTPVKFGISFTLTHMNQAGALV
                     HVYTDGSVQLNHGGTEMGQGLYLKVAQIVAAEFGIPLDRVKITATTTAKVPNTSPTAA
                     SSGTDLNGKAAQVAAQTIKQRMAEVAAVAFGVPPASIVFAGGKVAGGGQEISFGEAAK
                     LAHRARVSLSSTGYYSTPKIHYDTKTHTGRPFYYFAYGAALSEVEVDTLTGEYRLRRV
                     DILHDAGQSLNPAIDLGQVEGGFVQGMGWLTTEELWWDDKGHLRTHAPSTYKIPACGD
                     IPASFNVSLFKAGCNHEDVVYRSKAVGEPPLMLGIAVFQALRDAVAACGDGWTVPRFD
                     APATAERVLMAIETLRAGVAAPQSAASHAAASHARAAE"
     gene            1641..>2376
                     /gene="xdhC"
                     /locus_tag="VGC82_19725"
     CDS             1641..>2376
                     /gene="xdhC"
                     /locus_tag="VGC82_19725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HEY0425557.1"
                     /translation="MAVWLDALAAAEREHQPAVLVTVLSARGSTPREAGAKMVVSAGG
                     LAGTIGGGNLEYQCEAAARGLLADDAEGPSTRDFPLGPALGQCCGGHVTVLFEVIRPP
                     RLHVGLFGAGHVGKALVKLLADLPCRVSWIDSRPEALPANLPPNVLPVRVADPAQAVA
                     SLPAGSLIVVMTHDHQLDFEIVSAALRRADLLAVGLIGSNTKRARFVGRLARQGIAAD
                     GLICPIGLPGIEGNEPEIVAISVAAQI"
BASE COUNT          405 a          753 c          807 g          411 t
ORIGIN      
        1 tccctggtcg ccgtggcagc gtccctgttg gcgcagcgga cggggcggcc ggtgaagcat
       61 cggctggatc gcgacgacga catgatcctg accggcaagc ggcaccattg ccggatcgac
      121 tacgatgtcg gtttcgacaa cgacggacgc atccggggga tctcgttcgt tcaatccatc
      181 ggctgcggct actcgcccga tctgtccgga gccatcgccg accgtgcgat gtttcatgcc
      241 gacaacgcct attacctgga ccacgtccac atcgtttcgc accgctgcaa gacccacacc
      301 gtctccaaca ccgcgttccg cggcttcggc ggcccgcaag gcatgatggg gatcgagcac
      361 gtggtggacg agatcgcccg gcacctgcac atcgacccgc tgaccgttcg caagcgcaac
      421 ttctacggca cgcacgaccg caacgtcacg ccgtaccaca tgacgatcga ggacaacatc
      481 atctccgaag tcgtcgcgga actggaacgc gaagccggat atgccgtccg ccgtgaggaa
      541 gtcggcgcat tcaatgccgc caacccctgg atcaagcgcg gcctggcgct gacaccggtg
      601 aagttcggga tttccttcac gctgacgcac atgaaccagg ccggcgcgct ggtgcatgtt
      661 tacaccgacg gcagcgtgca attgaaccac ggcgggaccg agatggggca ggggctttat
      721 ctcaaggtcg cgcagatcgt ggccgccgag ttcggcattc cgctggaccg ggtgaagatc
      781 accgccacga cgaccgccaa ggtgcccaac acctcgccca ccgctgcgtc gtcgggcacc
      841 gacctgaacg gcaaggcggc acaggtcgcg gcgcaaacga tcaagcagcg catggcggag
      901 gtggcggcgg ttgcattcgg ggtgcccccc gcgtcgatcg tcttcgccgg cggcaaggtt
      961 gcgggcggcg gacaggagat aagcttcgga gaagccgcaa agctggcgca tcgggcgcgg
     1021 gtttcattat cctcgaccgg gtattacagc acgccgaaga tccactacga caccaagacc
     1081 cataccgggc ggccgttcta ctacttcgcc tatggcgcgg cgctgtccga agtggaggtc
     1141 gataccctga ccggggaata tcggctgcga cgagtcgata tcctgcacga tgctggtcag
     1201 tcgctgaatc ccgccatcga tctgggacag gtcgaaggtg ggttcgtcca gggcatgggc
     1261 tggctgacga ccgaggaact gtggtgggac gacaaggggc atctgcggac ccatgcgccc
     1321 tcgacctaca agattccggc atgcggcgac attccggctt cgttcaacgt gtcgctgttc
     1381 aaggcgggct gcaaccacga ggacgtggtc tatcgctcga aagcggtggg tgagccgccg
     1441 ctgatgctgg ggatcgccgt gttccaggcg ttgcgcgacg cggtggccgc gtgtggcgat
     1501 ggttggaccg tgccgcggtt cgacgcgccg gccacggccg aacgggtcct gatggcaatc
     1561 gaaaccttgc gcgcgggggt tgccgccccg caatctgccg cttctcatgc cgccgcttct
     1621 cacgcccggg cagccgagtg atggccgtgt ggcttgatgc cctggcggcg gccgaacgcg
     1681 agcatcagcc ggcggtgctg gtgacggtgc taagcgcgag gggttccacc ccgcgggaag
     1741 ccggcgcgaa aatggttgtg tccgccggcg gcttggctgg aacgatcggc ggcggaaacc
     1801 tggagtatca gtgcgaggcc gcggcgcgag gcttgctggc ggacgacgcc gaaggtccct
     1861 caacccggga tttcccgctc ggtcctgcct tgggccagtg ctgtggtggg cacgtgacgg
     1921 tgctgttcga ggtcatacgt ccgcccaggc tgcatgtcgg gctgttcggc gccggccatg
     1981 tcggcaaggc gctggtaaag ctgctggccg atctgccatg ccgtgtcagt tggatcgatt
     2041 ctcggcccga ggcgctgccg gccaatctgc cgcccaatgt tcttccggtg cgcgtggcgg
     2101 acccggcgca agcggtcgct tcattgccgg cgggaagcct gatcgtggtg atgacgcacg
     2161 atcaccaatt ggatttcgaa attgtctcgg cagcgctgcg gcgcgcggac ctgctcgcgg
     2221 tgggcttgat cggatcgaac accaagcgcg cccggttcgt cggccggctg gcgcgacagg
     2281 gcatcgccgc ggatggactg atctgcccca ttggcctgcc ggggatcgag ggcaacgagc
     2341 ctgaaatcgt cgcgatctcg gtcgcggcgc aaatcc
//
LOCUS       DASWQN010001155         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1300909, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001155 DASWQN010000000
VERSION     DASWQN010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300909"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(459..1738)
                     /locus_tag="VGC82_19730"
                     /pseudo
     CDS             complement(459..1738)
                     /locus_tag="VGC82_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048709925.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          277 a          634 c          572 g          279 t
ORIGIN      
        1 cggcaagcgc cggcaaaggc gacggaagca acgtggcgac gcacacgccc tcggcggctc
       61 gggcgctcac accaaacagc cgctatcatc cccaggcacg gtcccgaaga atggcacagc
      121 ccgaggcaag tgacccacta gttcggcggg atggggcgtg gcctgccatc ttcgtccaac
      181 gcgacgtagg taaagtcagc ggcggtcgcg tggaaccagg gcgcttctca caggcgaacc
      241 aagcttagcc agctgtccgt gatccgggga ccacctcaga ggctgaaaat gaatcgacgt
      301 ttgcccgccg agtgaaggat ttgcgcaatc tgccggcaac tgtggcatct cggcggggag
      361 agcgaggcgt tgcgaccgtt gtttgatggc gatgaagagg gtcgtcgttc tggcggtgag
      421 cgcagcagcg gatcgccgct atgtcggatg aggctgacct atgcctcgat tagccgccag
      481 aagcagacca tgttgtgggt cagcgcgtgc cacagagcgg tggccttggc cttctccacg
      541 ccgcgcacga gaacctgtgt cagccctcga ttgcgaacct gggcgttggc gcattccgcg
      601 gtcgccgcac gctgcttgta gatctgcttg gcctcggctt cgcccatgcg cctgcgccag
      661 tcggccactc caggcgggtc accggcgtgc ggcgcgtggc ggtcccggct ggcatcgcgc
      721 ggcttcggga ccggcgcaaa ggtcgcaacg ccgtgctgcg ccagggcctc gatggcgtcg
      781 aacttgacga agccgccgtc gaccagatgc tggcgtgggc gctcgccata gtcctcggcc
      841 agggcgtcgt tcatcggccg catcttgccc atgtcagagc cgatattgtc gaccgacaca
      901 ccggcgatcg cggtgctcat cgtgtccgtg gcgaactgga cgttgtaggc cggacggaag
      961 ccaccatcgg ccatcttcat cacccgcgcc tgcgcatcgg tcgtcgaggc gcgtggctcc
     1021 gtctcgcgcg cagccttgcc cttcgcagtc ccggtctcgt cctgcggcgc tgccttggcg
     1081 gcacgctcgg catcctggcg cgctttctcc tgctgccggg cgcgcagatc ctccgccacc
     1141 gccagtgccg ctcgcacccg ccgtgcgcga tcctcggccg cgcgtcgccg cgccgccgct
     1201 tggtggcgac tggccgcacc cggatcggcg tccagcgtcg cacgcaggtc ccgcagcgcc
     1261 tgctccgcct cgtggcggca cgccgccaag gtcgactgtc ggcgaaacga ggcggccccc
     1321 gccgcagcgc gcacccgcat cccatcctgg gcgacccgat cgaggcttgc cacgcccgtc
     1381 cgtaccagtg ccgtgaagct gtccaccagc aggcgctcca gcaccgcccc atggtcgacc
     1441 cggaagtccg ccagcgtctt ggcgttcatc ccaccccgcc acagagccac tggaatgcga
     1501 tgtgctcctc acagcagcgg gcaaccgcgc gggcgctgcc gatcccctcc accgtggcat
     1561 acagccaaag agccagcaga atgcgcgggt ccgctgacgg gtgacccggt cgaccggcca
     1621 ccgccttgat aggtgccagt agcggcgtca ggtcgagccc ctcggcgaaa ccccagacca
     1681 gacgcacccg gtggtcctcc gccaacagcc cgtccaggct gaccggtcgc cattccagtt
     1741 gatcacgctc ggcacagcga ag
//
LOCUS       DASWQN010001156         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1563159, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001156 DASWQN010000000
VERSION     DASWQN010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563159"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VGC82_19735"
     CDS             complement(<1..786)
                     /locus_tag="VGC82_19735"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0425558.1"
                     /translation="MNNVDSAHPMGLATPLALFFAAFFAIPLLLLLWVSLQGNATPGL
                     SQYARFFADGLGAKVLLHTMRIGLETTLGCLLLAYPLALLYRSAPGWARSLITLIVLL
                     PLLTSVVVRTFAWIVILGRQGLVNSLLQSLGMIEAPLRLLYTEAGLVAALIQVQMPLM
                     FLPLAGALVRIAENLEDASAALGAGAWRTFVRVTLPLSLPGALAGCTLTFAAAVTAFI
                     TQTLVGGGQMLLMPSYIYQQAITLQNWPFAAAISMIFLAAVLAV"
     gene            complement(808..1863)
                     /locus_tag="VGC82_19740"
     CDS             complement(808..1863)
                     /locus_tag="VGC82_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0425559.1"
                     /translation="MTKPEFSRRTLMGTALTLGAMQLFPGLSRAQARPLVFATFTGSW
                     EVAHKEVLVPAFRKANENAQIVLDPMLSVDQIAKVRAAQANPPIDVMLHDPGPALVAI
                     DQGLVAPYPVANSAHYANLIPTAQEPMGPAGFFQVVGLTYNPETVKTPPTSWADLWKP
                     EFKGKVGITNLNSTLGTGFMVEIARMHGGSEKDIEPAFKQIAALKPSLAAVASNPGAL
                     ASLYQQGQIDISPGNFNAIQILKAQGVPVEFVAPKEGAIAFKTTMHITRNSPNADLAF
                     KLIEAALSPEVQTKLMESPYLIVPTNSKVAMKGEIAKVLARGQDDLMKKFVFQDWKTI
                     NQGRSAWIDRFNREIAT"
     gene            complement(1892..>2197)
                     /locus_tag="VGC82_19745"
     CDS             complement(1892..>2197)
                     /locus_tag="VGC82_19745"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY0425560.1"
                     /translation="YKVALDIGGSILCGSPAVLVPGTAVRICLRPEALALTDNPDGLP
                     ATVEVGLPLGSTIVHELRLADGTALKSAAPRKIGDEPMLAGTKVRLRQQAPAIAFPT"
BASE COUNT          404 a          694 c          708 g          391 t
ORIGIN      
        1 gacagcgagc accgctgcca ggaagatcat cgaaatcgcc gcggcgaacg gccagttctg
       61 cagggtgatc gcctgctggt agatgtagct cggcatcaac agcatctggc ccccacccac
      121 cagggtctgc gtgatgaacg cggtgacggc ggcggcaaag gtcaaggtgc agccggccaa
      181 cgcaccgggc aggctcaacg gcagggtcac ccggacaaac gtgcgccacg cccctgcccc
      241 gagtgcggcc gaggcatcct ccaggttctc ggcgatccgc accagcgcgc cagccaatgg
      301 caggaacatc agcggcatct gcacctggat cagtgcggcg accaacccgg cctcggtata
      361 aagtaagcgc agcggggcct cgatcatgcc cagcgactgc agcagactgt tgaccaggcc
      421 ctgccgcccc aggatcacga tccaggcgaa ggtccgcacc accacgctgg tcagcagggg
      481 cagcagcacg atcagggtga tcagcgatct cgcccagcct ggagccgagc gatagagaag
      541 tgccaatgga taggccagca gaaggcagcc aagcgtggtc tcgagcccga tgcgcatggt
      601 gtggagcagc accttcgcgc ccaggccgtc ggcgaagaag cgggcgtatt gcgacagccc
      661 cggggtcgca ttgccttgca ggctgaccca cagcagcaac agcagcggga tagcgaagaa
      721 cgccgcgaaa aacagcgcca gcggcgtcgc caagcccatc ggatgggcgc tatcgacgtt
      781 attcatggcg tgacggcgcc ccggtcctca agtcgcgatc tcccggttga aacggtcgat
      841 ccaggcggag cggccctggt tgatcgtctt ccagtcctgg aaaacaaact ttttcatcag
      901 gtcgtcctgg ccccgggcga gaaccttggc gatctccccc ttcatcgcga ccttgctgtt
      961 ggtcggcacg atcaggtagg gcgactccat cagcttggtc tgcacctcgg gtgacagcgc
     1021 cgcttcgatc agcttgaacg cgaggtcggc gttcggggag ttcctggtga tgtgcatcgt
     1081 cgtcttgaac gcgatcgccc cttccttcgg cgccacgaac tccaccggca caccctgcgc
     1141 cttgaggatc tggatcgcgt tgaagttgcc tggcgagata tcgatctggc cttgctgata
     1201 cagcgacgcc agcgccccgg gattggatgc gacggcggcc aatgacggct tcagcgctgc
     1261 gatctgcttg aaggccggct cgatatcttt ctccgatccg ccgtgcatcc tggcgatctc
     1321 caccatgaag ccggtgccga gggtggagtt caggttggtg atcccgacct tgcccttgaa
     1381 ctccggcttc cacagatcgg cccacgatgt cggtggggtt ttcaccgttt ccggattgta
     1441 ggtcaggccg acgacctgga agaacccggc aggacccatc ggctcctgcg cagttggaat
     1501 caagttggcg tagtgggcgc tgttggcgac gggataaggc gccaccagtc cttgatcgat
     1561 cgcgaccagt gccgggccgg ggtcgtgcag catgacgtcg attggtggat tggcctgcgc
     1621 cgcgcgcact ttggcgatct ggtccaccga cagcatcggg tcgagcacga tctgcgcgtt
     1681 ctcgttggct ttgcggaagg ccggcaccag gacctctttg tgcgcgactt cccagcttcc
     1741 ggtgaacgtc gcgaacacca gcggccgagc ctgtgcgcgt gacaggccgg ggaacagttg
     1801 catggcaccg agcgtcaggg ctgttcccat caaggtccgc cgggaaaatt cgggtttggt
     1861 catgatgttt ccttcgtcgc gcgggaggac gttaggtggg aaaagcaatc gccggcgctt
     1921 gctggcgcag cctcaccttg gtgcccgcca acatcggttc gtcgccgatt tttcgcgggg
     1981 cggctgactt caacgctgtt ccatccgcca gccgaagttc atggacgatg gtcgaaccaa
     2041 gcggcaggcc aacttccact gttgccggaa gtccatcggg attgtcggtc agcgccagtg
     2101 cttccggccg caggcagatc cggacggcgg tcccgggcac caacacagcg ggcgagccgc
     2161 agaggatcga tccgccgatg tccagcgcga ccttgta
//
LOCUS       DASWQN010001157         4947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_508369, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001157 DASWQN010000000
VERSION     DASWQN010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508369"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VGC82_19750"
     CDS             <1..712
                     /locus_tag="VGC82_19750"
                     /inference="COORDINATES: protein motif:HMM:NF045129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY0425561.1"
                     /translation="LLAGINAGRLAGGQPAVWLDRTESYLGVMVDDLTTQGVAEPYRM
                     FTSRAEFRLSLRADNADLRLTEKAIGWGCVAAPRAVAFHSYQAALKQAMERARREDCP
                     AAAMERLGVVPAADGRRRSVLDLAGNESARWSLLCEVFPWLGALPARLAEQLRTDARY
                     AGYLHRQQAEVRLSRREDSVDLASVAFDEVGGLSTEMREKLNHARPDSLGAAARIQGI
                     TPAALASIAAFVRKRAAA"
     gene            780..3362
                     /gene="mutS"
                     /locus_tag="VGC82_19755"
     CDS             780..3362
                     /gene="mutS"
                     /locus_tag="VGC82_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY0425562.1"
                     /translation="MAQWFAVKADHPDALLFFRMGDFYELFFSDAEAAAAALDITLSH
                     RGEHAGTPIPMCGVPHHAAEVYLARLIRRGFRVAVVEQMEDPKFRTGKLPIKREVVRI
                     VTPGTITEESLLEAGRPNLLMALAWDKDGLGAAWVDVSTGLFETAKITKGELPGLLGR
                     LEPVEILAPQSLDLGDSGAKRSSETVPSTPLIARRRLAEAFGTVSLDAFGTFSDGEVV
                     AAALAVDYIRGTQSGALPRLAHPEPQGQAGVLLMDAATRASLEILRSRDGGTEHTLFA
                     AVQRTLTAAGARMLAAWLSAPLTDETTIVARQDAWSWCVANQDAANRLRGLLRTAPDM
                     ARALGRLSLNRGTPRDLAAIRDGLAAASGAAKVLASPLPPALDDARSAMPCDSDLARE
                     LQRALADPVPARLEDGGAIRSGFDGELDAHRTLRDKSRQVIAKLQLDFAQRFGVASLK
                     IKHHAQLGYIIEAPAVAVEKLRSFAELTLRQGMANGARFTTPELSELDQRIREAAEHA
                     TARERLVFGHLVRLVLAQADRLADCAEKLAYLDATQSIARLAEGGRWVRPVLTAAEEF
                     CVEAGRHPVVERALAGRAAFVPNRCDLSPDQRLMLLTGPNMAGKSTFLRQNALFVVLA
                     QAGLPLPAESAALGIVDRLFSRVGAADDLARGRSTFMVEMTETAAILHQAGPKSLVVV
                     DEIGRGTSTLDGLAIAWAVLEALHSAIRCRTIFATHFHELAELADRLPRLKPHTMRVK
                     EWKGSVVFLHEVAEGAGGRSWGVHVAELAGVPAPVVRRAASLMAAMEKHGGPLGKTAS
                     VQALPLFAAVAAPEEQGENGHETSPLCDALQQINPDNLSPKQALEALYRLKMLVAGCA
                     DDAT"
     gene            3376..>4947
                     /locus_tag="VGC82_19760"
     CDS             3376..>4947
                     /locus_tag="VGC82_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY0425563.1"
                     /translation="MLTPIPLPRAPEAAASLLSDALAALAEEGGTVSRDAAVGAFRRH
                     LARIQSYVQHAFEQEQITGLQAARLLGALIDGVVSALFEHTTTRTDLGAGNDLGIAAT
                     GGYGRGVLAPFSDIDLLFLTTEDPAPETLRVVEYMLYFLWDLGLKVGHATRSIKDCLV
                     EGGKDTTIRTALLDARFLTGNESLFGDFHRRFRAACKEAGAAEYIAAKQAERALRHRR
                     YGDSPFVVEPNVKEGRGGLRDLQTLYWIARYVFDTQTMGELADLGGILTQQEARHGRR
                     AWEFLWTVRFHLHYVAGRAEERLTFDLQPVVGARMGYTRHGRQDGVERFMRHYFLTAR
                     EIARLTRILEPAILRSALGPPALEAETDNALLAAGFVLAEGKLLPIAGRDFDAEPIQM
                     LRILQVARDRVLELHPLAIRSFIQNERRAVALRGDAAAAAVFMDLLCGKEPDRHRPGG
                     QHPDGAKWLSILNETGFLGRYLPDWARIVGQMQFDTYHIFTVDEHTIEAVRVLNTLER
                     GELAEVAPIASELVDH"
BASE COUNT          836 a         1494 c         1691 g          926 t
ORIGIN      
        1 acttctggcg ggtatcaatg ccggtcgcct ggctggaggt cagccggcgg tatggctcga
       61 ccgaacggag tcctatcttg gggtcatggt ggacgacctt accacccagg gcgtcgccga
      121 accgtatcgg atgttcacct cgcgggcgga attccgcctc agtttgcggg cggacaacgc
      181 cgatctccgg ctgaccgaaa aagcgatcgg ctggggctgc gtggccgcac cgagggcggt
      241 cgcctttcac tcctaccagg cggcgctgaa acaggcgatg gaacgtgcgc gccgggaaga
      301 ttgcccggcc gccgcgatgg agcggctggg cgttgtcccg gccgctgacg gacggcggcg
      361 gtcggtgctg gatctggccg gcaacgagtc tgcccgatgg agtctgcttt gcgaggtttt
      421 tccttggctc ggggctttgc cggcgcgcct ggctgaacaa cttcggacgg acgcgcgcta
      481 tgccggctac ctgcaccgcc agcaggcaga agtccggctg agccggcggg aggatagcgt
      541 ggacctggcg tccgtcgcgt tcgacgaggt cggcggcctt tcaaccgaaa tgcgcgaaaa
      601 actgaaccac gcgcggccgg actcgctggg agcagccgct cggattcagg gtatcacccc
      661 cgccgcgctg gcatcgatcg ccgccttcgt ccgcaagcgc gcggcggcat agtaaccggg
      721 tggacagcgt tccgaacaac ccgacgagcg tgccttcggc cgcgggggcg acgccggcga
      781 tggcgcaatg gttcgcagtc aaagcggatc atccggatgc gctgctgttt tttcggatgg
      841 gcgatttcta cgagttgttc ttcagtgacg cggaagccgc cgccgcggcg ctggatatca
      901 ccttatcgca tcgcggcgaa catgccggaa cgccgatccc gatgtgcggt gttccccacc
      961 atgccgccga agtttacctg gcgcgtctga tacggcgtgg gtttcgggtc gccgtcgtcg
     1021 agcagatgga ggatccaaag ttccgcaccg gcaagctgcc gatcaagcgc gaggtggtcc
     1081 ggatcgtcac gccgggaacg atcaccgagg aatctctgct ggaagcggga cggccgaacc
     1141 tgttgatggc tctggcgtgg gacaaggatg gtctgggtgc cgcctgggtc gatgtttcca
     1201 ccgggctgtt cgagaccgcc aagatcacca aaggcgaact tccggggttg cttggacggc
     1261 tggaaccggt ggaaatcttg gctccccagt cgcttgacct gggtgattct ggagcaaaac
     1321 gctcgtccga aacggtgccg tcaacgccgc tgatcgcgcg ccgccggttg gcggaagcgt
     1381 tcggcaccgt cagcctggac gcattcggca cattcagcga cggcgaagtc gtggctgcgg
     1441 cgctggcggt ggactacatc cgcggcaccc agtcgggcgc cctgccccgg cttgctcacc
     1501 ctgaaccgca gggtcaagcc ggcgtgctgc tgatggacgc ggcgacgcgg gcaagcctgg
     1561 aaatcctgcg ctcccgggat ggcggaacgg agcacacgct gtttgcggcg gttcagcgga
     1621 cgctgaccgc agcaggcgcg cggatgctgg cggcctggct gtcggcacct ctgaccgatg
     1681 agaccacaat cgtcgctcga caggatgcct ggtcatggtg cgttgccaac caggatgcag
     1741 ccaaccggtt gcggggtctc ctgcgaacgg cgccggatat ggcacgggcc ctgggccggt
     1801 tgtcgctgaa ccgcgggacg ccgcgggatc tcgcggcgat ccgcgatggg ctggcggcgg
     1861 cctccggcgc ggccaaggtt cttgcttcgc cgcttccgcc agcgcttgac gatgccaggt
     1921 cggcaatgcc gtgcgattcc gatctcgcac gggaattgca gcgcgcgctg gccgacccgg
     1981 ttccggcacg gctggaggac ggcggtgcga tccgctcggg atttgacggc gaattggatg
     2041 cacaccggac gcttcgcgac aaaagccggc aggtcatcgc gaagctgcag ctcgatttcg
     2101 cgcaacgctt cggcgtggcg agcctgaaga tcaagcacca cgcgcaactc ggctacatta
     2161 tcgaggcgcc cgcggtcgcg gtggagaaac tgcggagctt tgccgaactg accttgcgcc
     2221 aggggatggc gaacggcgcc cggtttacca cgcctgagtt gtccgaactg gatcagcgta
     2281 tccgggaggc cgcggagcac gccacggcac gggaacgcct ggtgtttggc catttggtcc
     2341 gtctggtcct ggctcaggcg gatcgactgg cggattgcgc cgagaagctt gcttatctgg
     2401 acgctacgca atcgatcgcc aggctcgccg aaggcggccg ctgggtcagg ccggtgctga
     2461 ccgctgcgga ggaattctgc gtcgaggccg ggcgtcatcc ggttgtggaa cgcgcgctcg
     2521 ccggtcgagc ggcgttcgtg ccgaaccgct gcgatctttc acccgatcaa cgcttgatgt
     2581 tgctgaccgg cccgaacatg gccggcaaat ccacatttct gcggcagaac gcattgttcg
     2641 tagtgctcgc tcaggcgggg ctgccgctgc ccgccgaatc cgccgcgctg gggatcgtgg
     2701 accggctgtt cagccgtgtc ggtgcggcgg acgatctggc ccgcggccga tcgaccttca
     2761 tggtcgagat gacggagacc gcagcgatcc tgcatcaggc ggggccaaaa tcgctggtgg
     2821 ttgtggacga aatcgggcgc ggcacctcga cgttggacgg tttggccatc gcatgggcgg
     2881 tcctggaggc tttgcactca gcgattcgat gtcgtactat tttcgccaca catttccatg
     2941 aacttgccga actggcagat cggctgccaa ggctcaagcc gcacacgatg cgggtcaaag
     3001 aatggaaggg ttccgtcgtg ttcctgcacg aagtcgcgga aggtgccggc ggacgatcat
     3061 ggggcgtgca tgtcgcggag cttgcgggcg tcccggcacc tgtcgttcgc agggccgctt
     3121 cattgatggc ggccatggaa aaacacggtg gcccgctcgg taaaacagcg tcggttcagg
     3181 cgctaccatt gttcgccgct gttgccgcac cagaggaaca aggggaaaac gggcatgaaa
     3241 cgtccccgct gtgcgacgct ttgcaacaaa taaatccgga taacctgtcg cctaaacaag
     3301 cactggaggc tttatatcgc ctaaagatgc ttgtggcggg ttgcgcggac gatgccacat
     3361 agtacgataa tcgagatgtt aacacctatc cccttgccgc gggcccctga agccgcggcg
     3421 tccctgctct cggacgcact cgccgcgctc gccgaagagg gcggcacggt ttcccgcgac
     3481 gctgccgttg gtgccttccg ccggcatctg gcccgcattc agtcttatgt gcagcatgct
     3541 ttcgagcagg agcagattac cgggctgcaa gccgcccggc tgttgggcgc cctgatcgat
     3601 ggggttgttt cggcgctgtt cgagcatacc accaccagga cggatctcgg ggccgggaac
     3661 gatttgggca ttgctgcgac cggcggctat ggccgtgggg tgctggcgcc attcagcgac
     3721 atcgacctgc tgttcttgac caccgaagat ccggcaccgg aaacgctgcg tgtcgtcgag
     3781 tacatgttgt actttctttg ggacctgggt ttgaaggtcg gccacgcgac gcgttcgatc
     3841 aaggattgcc tggtcgaggg cggcaaggac acgacgatcc gcacggcgct gctggacgca
     3901 cggttcctga ccggcaacga atccttgttc ggcgatttcc accgccgttt tcgggctgcc
     3961 tgcaaagaag caggtgccgc ggaatacatc gctgcgaagc aggccgaacg tgcattgcgc
     4021 caccgacgct atggcgacag cccgttcgtg gtcgaaccca atgtgaagga aggccgaggc
     4081 ggactgcgcg atctgcaaac cctttattgg atcgcgcgct atgtgttcga tacccagacc
     4141 atgggcgaac ttgccgattt aggagggatc ctgacgcagc aggaggcgcg gcatggcaga
     4201 cgcgcgtggg agttcctctg gaccgttcgt ttccatctgc attacgtggc gggccgggcc
     4261 gaggagcggc tgaccttcga tttgcagccg gtcgtcggag cgcggatggg ctatacgcgg
     4321 catgggcgtc aggatggcgt cgaacggttc atgcgccatt acttcctgac ggcgcgcgag
     4381 atcgctcggc tgacgcgtat tctggagcca gccatcctgc gatcggcatt gggtccgccg
     4441 gcactggaag ccgaaaccga caatgcgctg cttgctgccg ggtttgtcct ggcggagggt
     4501 aagttgctgc ccatcgccgg acgggacttc gacgccgaac caatccagat gctgcggatc
     4561 ttgcaggtcg cgcgcgatcg ggtcctggag cttcatcccc tagccatccg atcgttcatt
     4621 cagaacgaac gacgggctgt tgcgttgcgc ggcgatgccg ccgccgccgc ggtctttatg
     4681 gatttgctgt gcggcaagga gcccgatcgt catcgcccgg gcgggcagca tccggacggc
     4741 gccaagtggc tgagtatcct gaatgagacc gggtttttag gacggtatct gccggattgg
     4801 gcacgcatcg tcggccagat gcagttcgac acctatcata tcttcaccgt ggacgagcat
     4861 acgatcgagg cggtgcgtgt cctcaatacg ttggagcggg gggaactcgc ggaggtggcg
     4921 ccgatcgcat ccgagcttgt ggaccat
//
LOCUS       DASWQN010001158         1899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_905336, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001158 DASWQN010000000
VERSION     DASWQN010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905336"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..899
                     /locus_tag="VGC82_19765"
     CDS             24..899
                     /locus_tag="VGC82_19765"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY0425564.1"
                     /translation="MSQEAWGRWGIDDERGALNHIGPDQVRRATALVQTGQVVSLAQK
                     LSPRTPVPKHRAGIQHFMARDGGDYAAGARRPGGFQFAEDTVVMPLHIGTHIDALCHA
                     WCDDCLYNGFPGQGTRSTSGALRCGIDKMPPIVTRGVLLDLAGLHGGRLGASTSIGRA
                     DLIRAAEVAAVGIEAGDAVLIRTGWQEAQAGLVDISFDEEPGIDVEAALWLAESGVAM
                     VGADNFAIEAIPFPAGTVFPVHQRLIRDFGIPLLEGLVLQSLAEHRRGQFLFVAAPLP
                     VVGGTGSPLAPVAVL"
     gene            913..>1899
                     /locus_tag="VGC82_19770"
     CDS             913..>1899
                     /locus_tag="VGC82_19770"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0425565.1"
                     /translation="MSRLNSFLNGLRVIDVSAYLPGPFASLLLSDMGADVVKVEPPHG
                     DGMRLLGPRDPAGKPLFHAALNAGKSVRRIDLKSAGRKQEFLQLTDAADVLIEGFRPD
                     AMGRLGLDADTLRTRNPGLIVCSLSGYGAAGPRAQEAGHDGNYLALAGVLHRNGKPPR
                     FFDPPVADLTSSLFAALGILGAVQGRQRDGLGCHIDLAIADVAMPLQLFEIAALAATG
                     RVPQPETTYLNGGAAYYRVYSTSDQRHVVLGAVEPKFWHSFCTAAGRPDWIDRHAEAL
                     PQHALIADVAAYLTGLRLSECEARFAGADCCLSPVLDLAEALHGAHHQGRGLV"
BASE COUNT          286 a          603 c          667 g          343 t
ORIGIN      
        1 cgccacgaca cgggagcctc ggcgtgagtc aggaagcttg gggacgctgg ggcatcgacg
       61 acgagcgcgg cgcgctgaat catatcggtc ccgatcaggt acggcgcgcc accgccctgg
      121 tgcaaaccgg gcaggtcgtc agcctggccc agaagctgtc accgcgtacg ccggtgccga
      181 agcaccgcgc cggcattcag catttcatgg cgcgcgatgg cggcgactat gccgcggggg
      241 cgcgccgtcc gggtggcttc cagttcgccg aagacaccgt ggtcatgcca cttcatatag
      301 gcacccatat cgacgcgctg tgccacgcat ggtgtgacga ttgcctctac aacggttttc
      361 ccggccaggg cacccgcagc accagcggcg cgctgcgctg tggcatcgac aagatgccgc
      421 cgatcgtgac gcgcggcgtg ctgctcgatc tggccggttt gcacggtggt cggctgggcg
      481 ccagtacttc gatcggccga gccgacctga tccgggcggc ggaggtcgcc gcggtgggaa
      541 tcgaggccgg cgatgcggtg ctgatccgca ccggttggca ggaggcgcaa gccggactgg
      601 tggatatttc gttcgatgag gaacccggca tcgatgtgga ggcggcgctg tggctggcgg
      661 aatccggtgt ggccatggtg ggcgccgaca actttgcgat cgaggccatt ccgttccccg
      721 ccgggacggt cttcccggtg catcaacgac tgatccgcga cttcggcatt ccgctgctgg
      781 aaggtctggt gttgcagtcg ctggcggaac atcggcgcgg ccagttcctg ttcgtcgccg
      841 ccccgttgcc cgtcgtcggc gggaccggaa gcccgctcgc gccggttgcg gtgctgtagc
      901 aatgagtcgg cgatgtcacg gctgaacagc ttcctgaatg gtctgcgggt gatcgatgtc
      961 tcggcctatc tgccggggcc attcgccagc ctgctgctgt ccgacatggg cgcggacgtg
     1021 gtgaaggtcg aaccacccca cggcgacggg atgcgcctgc tcggaccgcg tgatccggcg
     1081 ggcaagccat tgttccatgc ggcgctgaac gccggcaaga gcgtgcgccg aatcgatctg
     1141 aaaagcgccg gcaggaaaca ggaatttctg caactgacgg atgcggcgga cgtgctgatc
     1201 gagggttttc ggccggatgc gatggggcgt ctcggtcttg acgcggatac gttgcggacg
     1261 cgcaatccgg gtctgatcgt ctgttcgctc agcggttacg gcgcggcggg tccgcgcgcg
     1321 caggaagccg ggcatgacgg caactacctg gccctggccg gggtgctgca tcgcaatgga
     1381 aaacctccgc gcttcttcga tcccccggtt gccgatctaa ccagttcgct gttcgcggca
     1441 ctcggcatcc tgggtgcggt gcagggacga cagcgagacg ggctgggttg ccacatcgat
     1501 ctcgccatcg ccgacgtggc gatgccgttg cagctattcg aaatcgccgc gttggccgcg
     1561 accggccgcg tgccgcaacc ggaaaccacc tatctgaatg gcggcgccgc ctattatcgc
     1621 gtgtattcca cgtccgacca gcggcacgtc gtgcttggcg ccgtcgaacc gaagttctgg
     1681 cacagcttct gcaccgccgc cgggcggccc gattggatcg accggcacgc cgaggcgttg
     1741 ccgcagcacg cactgatcgc cgatgtggcg gcatacctca ccggccttcg gctgtccgaa
     1801 tgcgaggccc ggtttgccgg cgcggattgc tgcctgtcgc cggtgctcga tctggccgag
     1861 gcgctgcatg gggcgcacca tcagggccgt ggcttggtt
//
LOCUS       DASWQN010001159         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1960848, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001159 DASWQN010000000
VERSION     DASWQN010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960848"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1866
                     /locus_tag="VGC82_19775"
     CDS             <1..>1866
                     /locus_tag="VGC82_19775"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0425566.1"
                     /translation="ADLAARLPFRNFCWERIGSDGKRHFMTISGDPVYDRNGAFSGYR
                     GTGREITAEVEAKARLAQANAELETGRRRFDAVLSNITQGVCFFDGDERLLLCNRRYA
                     EIYGLPPEATRAGLTYDEVVSHRLAGGSAPDMSPSDYRAWRDQIAATGQPTNTVLALQ
                     NGRFIAIYYQPMPGGGWVATHEDITERHKAEASIVFMARHDALTKLPNRMFFRERIDQ
                     AIAMAGRGSKFAILCLDLDKFKQVNDTMGHPVGDGLLIAAAERLQACAREVDTVARLG
                     GDEFVIIQSDIRQRDDAEVLASRIIAAFRQPFDVGGHQIVAGVSIGVTVAPDDSVSYE
                     TLMRDADIALYLAKTEGRGTARFFEPQMDARIHARRVLELDLHGAMARNEFELYYQPQ
                     INLIHNKVSGFEALLRWHHPVRGLVSPFDFIPFAEETGLIVAIGDWVLHTACFEAENW
                     PDDVHVAVNLSPVQFKKGDLVATVRAALAASGLRPDRLDLEITESVFLSDTAETLAAL
                     HQLRAMGIGVALDDFGTGYSSLSYLRSFPFNKIKIDQSFVRDLMTNKGSISIVRAVTG
                     LGQSLGMTTIAEGVETLEQLERLREEGCNEVQGYYFSHPKTASEVPLMIEGLQQVS"
BASE COUNT          363 a          563 c          597 g          343 t
ORIGIN      
        1 gctgatctcg ccgccaggct tccgttccgc aatttttgct gggagcggat tggttcggac
       61 ggcaagcgcc acttcatgac catcagcggc gaccctgtgt acgaccgaaa cggcgccttc
      121 agcggatatc gcgggaccgg ccgcgagatt accgccgaag tggaagccaa agctcggctg
      181 gcccaggcca acgcagagct tgaaacaggc cggcggcgtt tcgacgcggt gttgagcaac
      241 ataacgcaag gcgtctgctt cttcgatggc gacgaaaggc tgctgctgtg caaccggcgt
      301 tatgccgaga tctatggtct gccgccggag gcgacccgcg ccggactgac atacgatgag
      361 gttgtaagtc atcggcttgc cgggggaagc gcgccggaca tgtcgccgtc ggactatcgc
      421 gcatggcgcg accagatcgc cgccaccggc cagcctacca acacggtcct agcactgcaa
      481 aatggccggt ttatcgcgat ctactatcag cccatgcccg gtggcggctg ggtagcgacc
      541 cacgaggaca tcaccgagcg gcacaaggcg gaggcgagca tcgtgttcat ggcccgccat
      601 gacgcattga ccaagctgcc taaccggatg tttttccggg aaagaatcga ccaggccatc
      661 gccatggccg gccgcggaag caagttcgcg atcctttgcc tcgatctgga caagttcaag
      721 caggtcaacg acacgatggg tcaccccgtc ggcgacggtc tgctaatcgc cgcggcggaa
      781 aggctgcagg cctgcgcgcg cgaggtggac accgtcgctc gcctgggcgg cgatgagttc
      841 gtcatcatcc aatcggacat ccgccagcgc gacgatgccg aggttctggc gagccggatc
      901 atcgccgcat ttcgtcagcc cttcgacgtc ggcggtcacc agattgtcgc gggggtgagc
      961 atcggcgtca ccgtggcacc ggacgatagt gtttcgtacg agacgctcat gcgggacgct
     1021 gacatagcac tttacctggc aaagaccgag ggtcgcggca ccgcccggtt cttcgagccg
     1081 cagatggatg cgcgcatcca cgcgcgccgc gtgctggagc tggatttgca cggtgcgatg
     1141 gcccgcaacg agtttgagct ctattatcag ccgcagatta acctgatcca caacaaggtg
     1201 tcgggattcg aggcactgct gcgctggcat cacccggtgc gcggcctggt ttcgccattc
     1261 gatttcatcc ccttcgcgga ggaaaccggc ctgatcgtgg cgatcggcga ctgggtgttg
     1321 cacacggcct gtttcgaggc tgaaaactgg ccggacgacg tacacgtcgc ggtcaatctg
     1381 tcaccggtgc agttcaagaa aggcgatctt gtcgcgacag tgcgggcggc acttgccgca
     1441 tcgggcttgc ggccggaccg gctcgacctg gaaatcaccg agtcggtgtt tctgagcgac
     1501 accgccgaga ccctggcggc gctgcatcag cttcgagcaa tggggattgg cgtcgcattg
     1561 gatgatttcg ggacggggta ttcgtcgctt agctacctgc gcagctttcc gttcaacaag
     1621 atcaagatcg accaatcctt cgtgcgcgac ctgatgacga acaaggggtc catttcgatc
     1681 gtccgcgcgg tcaccggcct cggtcaaagc ctggggatga ccacgatcgc cgaaggcgtg
     1741 gagacgttgg aacaattgga acggctgcgc gaagagggat gcaacgaggt ccagggttat
     1801 tacttcagcc atcccaagac cgccagcgag gtcccactga tgatcgaagg cctgcagcag
     1861 gtcagc
//
LOCUS       DASWQN010001160         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1564206, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001160 DASWQN010000000
VERSION     DASWQN010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564206"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VGC82_19780"
     CDS             complement(<1..670)
                     /locus_tag="VGC82_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY0425567.1"
                     /translation="MSEFLKPVSDDEIHAYVDDQLQVERRSVVKTYLDENTEAAKRVA
                     AYEAQRAAIRAAFSSNLTEPLPEKLSLARIIAERNTRRPPIWLLAASVAFALGIGLAG
                     GYLLHATQGQTQNPLAFLEQEAFTSHVVYAVDRRHPVEVSSAETPHLQQWLSNRLDRM
                     VVAPDLSSLGFHLIGGRLLATEHGEAAALLMYDDADHRRISLLLRPMAPAMKMPNAMI
                     EQNGV"
     gene            complement(702..1190)
                     /locus_tag="VGC82_19785"
     CDS             complement(702..1190)
                     /locus_tag="VGC82_19785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0425568.1"
                     /translation="MRPLVSGPIGAIESCIPALRRYAGALLRDRQEVDDLVHDCLVRA
                     LDRLHTRRNDAGMRPWLFTIMHNLYVSRIRQNRSHRPIESLDNVAEAALAVDAPQDHV
                     IRTQDVMRAVQRLPEDLRSVLLLVTVEDLSYGDVARVLGIPVGTVMSRLSRARERVRR
                     DS"
     gene            2016..>2862
                     /locus_tag="VGC82_19790"
     CDS             2016..>2862
                     /locus_tag="VGC82_19790"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0425569.1"
                     /translation="MATATTFRTETRAWLEENCPPAMRSFMPEHEQVAGGKQARYVRP
                     EQKQWLDRMAAKGWTAPTWPTRYGGGGLSNDEALILEEEMQDLGCRAPLRGMGLSMLG
                     PVLLEYGTEEQRQTHIPPIVRGEIRWCQGYSEPEAGSDLASLRTRAVLQGDHFLVNGH
                     KIWTSNAHVSDWIFCLVRTDPTAKKHEGISFLLIDMDDPGVRPRPIRLISGASVFCET
                     FLENVRVPAGNLVGPLNKGWTIAKRLLEHERKGIGGIAARNAAKFEIPLEAVAKRFAG
                     ESEGKI"
BASE COUNT          575 a          881 c          844 g          562 t
ORIGIN      
        1 tgactccatt ctgctcgatc atggcgttgg gcattttcat ggcgggagcc atgggccgca
       61 gcagcaagga aatccgcctg tgatcggcat cgtcgtacat caaaagcgcc gcggcctcgc
      121 catgttcggt cgcgagcaat ctgccgccga tcaggtgaaa tccaagtgac gacagatcgg
      181 gtgccaccac catccggtcg aggcgattgg aaagccactg ctgcagatgg ggtgtctctg
      241 cgctcgagac ttcgacagga tgacgccgat cgactgcata caccacatgg ctggtgaacg
      301 cttcctgttc cagaaacgcc aatgggttct gcgtctgccc ctgtgtcgca tgcaacaggt
      361 agccgcccgc caggccgata cccaaggcga acgcgacgga agcggccaaa agccagattg
      421 gcggccgacg cgtgttccgc tccgcgatga tccgggcaag cgaaagtttt tccggaagcg
      481 gttccgtgag attggacgaa aatgcggcac ggatcgccgc ccgctgtgcc tcataggcgg
      541 cgactcgttt cgcggcctcg gtgttttcgt caaggtaggt cttgacgacg gaccgacgct
      601 caacctgcag ttggtcgtcc acgtaggcgt gtatttcatc gtcgctgacc ggcttcaaaa
      661 actcgctcat ttcaatcgcc tgaactcaga cgcccccgtt ttcatgaatc tcgcctcaca
      721 cgttcccggg ccctggacaa acgtgacatc accgtgccga ccggaatacc gaggacgcgg
      781 gcgacgtcgc cataggaaag gtcctctacg gtaacaagaa gcagtacgct gcgaagatcc
      841 tcgggcaaac gctgcacggc acgcataacg tcctgtgtcc gaataacgtg gtcctggggg
      901 gcatccacgg cgagtgccgc ttcggcgaca ttatcgagcg attcgatcgg ccgatgcgat
      961 cggttctgac ggatgcggct tacatacagg ttatgcatga tggtaaacag ccagggccgc
     1021 atcccggcat cgttgcgtcg cgtatgcaac ctgtcgaggg cccgcacaag acaatcgtgc
     1081 actaggtcgt ccacctcttg ccggtcgcgg agcagggcgc cggcatatcg tctgagggcc
     1141 ggaatgcacg attctatcgc tcctattggt ccgctcacca gcggccgcac tgctgcccga
     1201 aggggcagcc gcgctcacgg tgaactccgg gcatctggat cgggcgcgac atggctgctt
     1261 ggtctctcga aaacgcacac caaggatgct atgccaatat tccgcaatcc gaaatagatg
     1321 gttgcacgga atcttacctg ccgacgatcc aacgacgatc atttgatata cttcaagtag
     1381 agcggacact atcgccggtt cgtcagtcct cgacatatta ttatagtgcg acaactattc
     1441 gctttcagag caaaaaagac ccttccgggt acgatgttcc ggttcatggc agccaaccca
     1501 tcaagcccta aagcgtcctc gtcccgacgc tgcgtccgac ccatgttact attcgagcag
     1561 cggtcgaact tcaggacagc agtttattcc ccagacgtta ctgctgtcgc ggcgtggttg
     1621 cgacggcagt aacggttgga tccaagccgc cggcccatcg acataatgcc gttcttcgca
     1681 cgttctgtgg gttcccaagc ggatggtcgg aacactgcgt gcatgcggtt agtcgtctat
     1741 atcataaacg cggccggcag ggacttattc catgcgtcgc gtagtttttt gcaagagaat
     1801 tttcgccaag cgcctctcgc caggggaatt cttgctacgt gagctttgcc ggtggttgtt
     1861 tttcagcgat cgccggagtg ggcggcttca accgttcgaa atacctgctt cggcgccgcc
     1921 gccatgtcgt ccggctacca tgtcgtccgg cacttgcccg tcggtgggga tgatggcact
     1981 tcatcgctgt aagggaacct cgaaggaacc gctccatggc gaccgcgaca acattccgca
     2041 ccgaaacgcg ggcatggctg gaggagaact gtccccccgc gatgcgcagt ttcatgccgg
     2101 agcacgaaca ggtcgccggc ggcaagcaag cccgttacgt tcgccccgaa caaaaacaat
     2161 ggctcgatcg catggcggcc aagggctgga ccgcccccac ttggccgacc cggtacggcg
     2221 gcggcggcct gtccaacgat gaggccctga tccttgaaga ggagatgcaa gacctgggct
     2281 gccgagctcc tttgcgcggg atgggtctgt ccatgctggg tcccgtgctg ctggaatacg
     2341 gcaccgagga gcagcgccag acccacatcc cgcctatcgt tcgcggcgag atccgctggt
     2401 gccagggcta cagcgagccg gaagccggat ccgacctcgc cagcttgcgc acccgggcgg
     2461 tcctccaggg cgaccacttc ctggtcaacg ggcacaagat ctggacctcc aacgcgcatg
     2521 tttccgactg gatattctgt ctcgtccgca ccgatcccac ggccaagaaa cacgagggta
     2581 tcagcttcct gctgatcgat atggacgacc ccggggtgcg ccctcgcccg atccggctga
     2641 tcagcggcgc ttcggtgttc tgcgaaacgt tcctggagaa cgtccgcgtt cccgccggca
     2701 atctggtcgg gccgctcaac aagggttgga ccatcgccaa gcgccttctg gaacatgagc
     2761 gcaaaggcat cggtggcatc gccgcccgca atgcagcgaa attcgagatc ccgctggagg
     2821 ctgtcgcgaa gcgtttcgcc ggcgaaagcg agggcaagat cg
//
LOCUS       DASWQN010001161         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2225641, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001161 DASWQN010000000
VERSION     DASWQN010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2225641"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VGC82_19795"
     CDS             <1..894
                     /locus_tag="VGC82_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425570.1"
                     /translation="LTAVIVAVTEGQPRVLTIERGLALPSGPFEPGHRSLQAGLRSWV
                     ETQTHHPLGYVEQLYTFADQDRKQGALGRVVSVSYLGLTREAPVIEGWDAAWQPWYRF
                     FPWEDFRGSGAALGESILAPPLYAWAEAANDPAIRRDRERRITINFGLRGQRWNEDLV
                     LQRYELLWEAGLVAEARPHAEGLTDAWGEPMRHDHRRILATGIARLRAKIKYRPVVFE
                     LMPPDFTLLRLQRTVEALAGRLLHKQNFRRLIDQQDLVEETGEVTTETGGRPAKMFRF
                     RREVLAERQVAGTKLPLVRAL"
     gene            complement(1003..1167)
                     /locus_tag="VGC82_19800"
     CDS             complement(1003..1167)
                     /locus_tag="VGC82_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425571.1"
                     /translation="MSWPSGGQWEWAIGEFIVLGFLFWELYRLHRSQRLDREKARQDR
                     DSGSDLPARE"
     gene            complement(1164..1514)
                     /locus_tag="VGC82_19805"
     CDS             complement(1164..1514)
                     /locus_tag="VGC82_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425572.1"
                     /translation="MYDWLGIGLIALGAGLVVAGIIKRRSTTRSPLPAGSIRPEFAAM
                     GEMVRPLILFAVGLFALKMSFFYFVLGGQGLLTPLDFAGIMFVLAAYSGYLVAATTIR
                     PVEQPTAAAVKSTA"
BASE COUNT          312 a          520 c          496 g          276 t
ORIGIN      
        1 ttgaccgccg tcatcgtcgc ggtaaccgag ggacagcctc gggttctgac catcgaacgc
       61 ggcctggcac tgccctcggg gccattcgag cctggacatc gctcgcttca ggcgggactc
      121 cgatcctggg tggaaacgca aacccatcac ccgctgggct atgtcgagca actttacacc
      181 tttgccgacc aggaccggaa gcagggcgca ctcgggcgcg ttgtgtcggt cagttatctg
      241 ggcctgacgc gggaagcacc ggtgatcgag ggctgggacg ccgcctggca accctggtac
      301 cggttctttc cgtgggagga tttccgcggg tcgggagccg ctttggggga atcaattctg
      361 gcaccgccgc tctacgcctg ggccgaagct gccaatgatc cggcgatccg gcgcgaccgc
      421 gaacggcgga tcacgatcaa cttcggcttg cgcggccaac gatggaacga agacctcgtg
      481 cttcaacgct acgaactact gtgggaagcc ggtcttgtcg ccgaggctcg gccgcatgcg
      541 gagggcctga ccgatgcctg gggcgagcca atgcgccacg accatcgccg catcctggcc
      601 acgggaattg cgcgcctgcg cgcgaagatc aagtaccggc cggtggtttt tgaactgatg
      661 ccgccggact tcacgctgtt gcggttgcaa cggactgtcg aagccctggc cgggcgcctg
      721 ctgcacaagc agaatttccg ccggctgatc gatcagcagg acctggttga ggaaacaggt
      781 gaagtaacga ccgagaccgg gggccggccg gccaagatgt tccgatttcg tcgtgaagtg
      841 ctcgcggagc gccaggtggc aggcaccaag ctgccgctgg tgcgggcatt gtaaaacctg
      901 gagcgtgatc acgctggccg gaacgtccga agcgtgtcaa tcacgggaca aaacaagcgg
      961 gcagaccatt tgcgcctgca cagtcaggct gagatggtct ggtcactccc gagcgggcag
     1021 atcgcttccg ctgtcccggt cctgtcgcgc cttctcccgg tcgagccgtt ggcttcggtg
     1081 caggcggtag agttcccaaa acaaaaaacc cagcacgatg aactcaccga tcgcccattc
     1141 ccactgcccg ccgctcggcc agctcatgcc gtcgatttga cggcggcggc agtgggctgt
     1201 tcgaccggcc ggatcgtcgt cgcagccacc agatacccac tataggctgc cagcacgaac
     1261 atgatcccgg cgaaatcgag tggcgtcagc aagccctggc cgcccagcac gaagtagaaa
     1321 aaggacatct tcagtgcgaa aagacccacg gcaaaaagga tcagcggccg gaccatctct
     1381 cccatcgcgg cgaactccgg acggatcgac ccggccggca gcggcgatcg tgtcgtgctc
     1441 cggcgcttga tgatgccggc aaccaccaag cctgctccga gtgcgataag tccgatcccc
     1501 aaccagtcat acatctcacg cctccttcaa agttgtacgc gctcccgttg ttccagccgc
     1561 tcgaaaagat ccagtgtcca ccgcatcctg tccgccacgg ccca
//
LOCUS       DASWQN010001162         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1564384, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001162 DASWQN010000000
VERSION     DASWQN010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564384"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VGC82_19810"
     CDS             <1..268
                     /locus_tag="VGC82_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425573.1"
                     /translation="DSMKDQLRRDVASWREGRADSSSRDATGLSVAVTRSIVPPTNAR
                     MKRAKPASAEIKPASSAEKKPFGMSYDGTSRADYIKLMISRGAR"
     gene            304..474
                     /locus_tag="VGC82_19815"
     CDS             304..474
                     /locus_tag="VGC82_19815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425574.1"
                     /translation="MVVQLRHVVAIYDEQGAVKLATTAGGVHVLKDITVQRAAGIVSR
                     AEDASTATKSDQ"
     gene            complement(769..1965)
                     /locus_tag="VGC82_19820"
     CDS             complement(769..1965)
                     /locus_tag="VGC82_19820"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0425575.1"
                     /translation="MSARPFTGVRILDFTQVLAGPYASYQLALLGADVIKIERRGGED
                     MRRTPLSREWAERNMAPPFLAVNGNKKSLTLDLQKPAAREIVLKLAAKADVVMENFRA
                     GVMDRLGLGYAALSAINPRLIYCAISGFGQTGPARGEAGYDGKIQAMSGIMAITGHEE
                     MGPTRAGFAVCDVLSGATGAFAIASALFQRTHTGIGQYLDVSMLEASLAFLATQVADY
                     TVAGHHQHQAGNQAISRKVTANLFRAKESFLLLAVNDEKQYRNLMTALGRADVLDDPR
                     FSDWFQRKANEAALRSVIESALAADDAKSWERRLNDAGAPCASIWKIEEIIDHPQITV
                     RGAMQTVDSPYGPLRLMGSGFQMAHGGGKLDSVAPEAGADTDTVLAEIGYDSAAIAAL
                     RAEAIV"
BASE COUNT          355 a          688 c          661 g          410 t
ORIGIN      
        1 cgactccatg aaggatcagt tgcgtcggga cgtcgcatcc tggcgcgaag ggcgggccga
       61 cagttcttca cgcgacgcaa ccggcctcag cgttgcggtc acacgctcca tcgtgccgcc
      121 gacgaacgcg cgcatgaagc gcgccaagcc cgcctcggcg gagatcaagc cggcatcctc
      181 ggcggaaaag aagccgttcg gcatgtcgta tgatggcaca agccgggctg actatatcaa
      241 gttgatgatc tcccgcggcg caaggtgagc tgggttacgt tcgaagctgc cgctggcggc
      301 ttgttggtgg tgcaacttcg acatgtcgtc gcgatctacg acgagcaggg cgctgtcaag
      361 ctggcgacca cggcgggcgg ggttcatgtt ttgaaagaca tcaccgtgca acgggccgcc
      421 gggatcgttt cccgggctga agacgcttca acagcgacga agtccgatca atgattttcg
      481 aaccggtact gcaaccgtca tatgcacggc tgctggcgtt atggtattgt gggtgatggc
      541 agatacttca tatcggatcg ttcaccgcga ggatgatagc ttcgccgttg aaatcgtcag
      601 gttcggcgca ctgccgcaag ttgctgccgg gtttgccacc gaagccgccg cgcaaggttg
      661 gatcgcccag gaccaacggt tccggtcctc cggggagacg tcaagtacct cggctggccg
      721 gagatggcgc agccagtgac gatcttgccg gctgttgcgg cgccacatct agacgatcgc
      781 ttcggctcgc aaggcggcga tcgccgcgga gtcataaccg atctccgcta gtacggtatc
      841 ggtatcggct cctgcctcgg gtgccacgct gtccagcttg ccgccgccat gggccatctg
      901 gaagcccgaa cccattagtc ggagcggccc gtaagggctg tccaccgtct gcatcgcgcc
      961 ccgaacggtg atctgcggat ggtcgatgat ctcctcgatc ttccagatgc tggcgcaggg
     1021 cgctccagca tcgttcaggc gccgctccca gctttttgca tcgtccgcgg ccagagctga
     1081 ttcgataacg gaccgcagcg ccgcctcgtt cgccttgcgc tgaaaccagt cgctgaagcg
     1141 ggggtcgtcg agcacatcgg cgcggccgag cgcggtcatc aggttgcggt actgcttttc
     1201 gtcattcacc gcgagcaaca gaaagctttc cttcgcgcga aacaggttgg cggtcacctt
     1261 gcggctgatc gcctggttgc cggcctgatg ctgatggtgt cccgccacgg tgtagtccgc
     1321 cacctgcgtc gccaggaagg ccaggctggc ctccagcatc gacacatcca ggtactgccc
     1381 gatgcccgta tgggtgcgct gaaacagcgc cgaggcgatc gcgaacgccc ccgtcgcgcc
     1441 cgacaatacg tcgcataccg cgaacccggc tcttgtgggc cccatttcct catgcccggt
     1501 gatcgccatg atgccggaca tcgcctggat cttgccgtcg tagccggctt cgccacgggc
     1561 cggtccggtc tggccgaagc ctgagatggc acagtagatc agccgcgggt tgatcgcgga
     1621 cagcgcggca tatcctagac ccagccggtc catcacgccg gcgcggaaat tctccatgac
     1681 gacgtcggcc ttggccgcca gcttcagcac gatttcccgc gccgccggct tttgcagatc
     1741 cagcgtcagg cttttcttgt tgccgttcac cgcgaggaac ggcggcgcca tgttgcgttc
     1801 ggcccattcg cgcgacaacg gcgtgcggcg catgtcctcg ccgccgcgcc gctcgatctt
     1861 gatcacgtcc gcgcccagca gtgccagttg gtaactggca tacggaccag ccagcacctg
     1921 ggtgaagtcc agaatccgga cgccggtgaa cgggcgcgcg ctcatgccgc cttcaccgtg
     1981 cgattgggcc gagcgagctc tttctgccgg tcgaattctt cccgccggcg cctgagttcg
     2041 gcctcgccga tctgttccag gttcaggtag tccagcttcc acttgccatc cgcatcccag
     2101 cggagcggcg actg
//
LOCUS       DASWQN010001163         2214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2622365, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001163 DASWQN010000000
VERSION     DASWQN010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2622365"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VGC82_19825"
     CDS             complement(<1..977)
                     /locus_tag="VGC82_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0425576.1"
                     /translation="MAKPKLLIVEDDEGLCSQYRWAFPACEILMANARPPAVALVKRE
                     NPPVAIMDLGLPPDPDGVSEGFATLTEVLRIAPQTKIIVVTGNGERKNALRAVALGAY
                     DFCEKPVEIEVLRTIIERGLNLHRLEEENRRLATVPVRSSIERIVTASPGMLKVCRDI
                     EKIATTNVPVLLLGESGTGKEALARAVHDLGPRAKQPFVAINCGAIPENLLESELFGH
                     ERGAFTGAVKQTIGKIESAHKGTLFLDEVGDLPHPLQVKLLRFLQDQVVERIGGRQKI
                     QVDVRIVSATNADLDDKISQGVFRGDLLYRMNAFTIRIPPLRQRGADIAL"
     gene            complement(990..>2214)
                     /gene="prsK"
                     /locus_tag="VGC82_19830"
     CDS             complement(990..>2214)
                     /gene="prsK"
                     /locus_tag="VGC82_19830"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HEY0425577.1"
                     /translation="TSGSTRSRIRSMVVENFFSNRYNYRREWMRCIDALTAPEAFVAL
                     HKRAIRAAAEVVDSPAGALFLRPPSDVAFQWAGSWNMPSVTAPVPPGHPLVALFRDGD
                     WIVHLDEHADAEKWLPELVRSWVVVPLNHFGGLIGFVVLARSRAHFKLDREAYDLLRV
                     VGREIASRVAEQRAMQVLTQTRQLREYSQRFAFVIHDIKNVSGQLSMLLTNAEVYADN
                     PEFQRDMLATVRASVAKITRLLSRLQADRQERDHALITPSDRLRELVEANRVASQREI
                     RFHDHGFSAGAAIDPDAFDAVVTHLLNNALEASDTHAAVEIDLRMEAHGVVVDIIDQG
                     PGMAPEFVRDELFRPLRSTKGDGHGIGAFQARELLRDAGGDLLVISKPNAGTTMRLIL
                     PLVRPGVAEAASAET"
BASE COUNT          414 a          699 c          676 g          425 t
ORIGIN      
        1 agggcgatgt cggcgccgcg ctgacgcaaa ggcgggatcc ggatcgtgaa ggcgttcatg
       61 cgatagagta ggtcgcctcg gaacacgccc tggctgatct tgtcgtccag gtcggcattg
      121 gtggccgata cgatgcggac atcgacctgg attttctgcc gtccgccgat ccgctcaacg
      181 acctggtcct gcaggaagcg cagcagcttg acctgcagcg ggtgcggcag atcgcccact
      241 tcgtccagga acagcgtccc cttgtgggcg ctttcgatct tgccgatggt ttgcttgacc
      301 gcgccggtga aggcgccacg ctcatgaccg aacaactcgc tttccagcag gttctccgga
      361 atggcgccac agttgatcgc gacgaaaggc tgcttggccc ggggacccag gtcatgcacc
      421 gctcttgcca gcgcttcctt gccggtgccg ctctcaccca gcagcaggac gggaacattg
      481 gtcgtcgcga tcttttcgat atcgcggcag accttcagca ttcccgggct ggcggtcacg
      541 atccgttcga tcgaggagcg aaccggcaca gtggcaaggc ggcggttttc ctcctccagc
      601 cggtgcaggt tcagcccgcg ttcgatgatg gtgcgcaaca cctcgatctc gaccggtttt
      661 tcgcagaaat cataggcgcc cagggcaacc gcccgtagcg cgttcttgcg ttcgccattg
      721 ccggtgacga cgatgatctt ggtctgcggc gcgatacgca gcacctcggt cagcgttgcg
      781 aacccttcgc tgaccccgtc gggatctggc ggcaggccaa ggtccatgat ggcaacaggt
      841 ggattctcgc gcttcaccag cgcaaccgct ggcggtctgg cgttcgccat cagtatttcg
      901 caggcaggaa atgcccagcg atactgcgag caaagcccct cgtcgtcctc gactatcagc
      961 aatttcggtt ttgccatcaa tggctccggt cacgtttcgg cggatgcggc ctcggcgact
     1021 ccgggcctaa ccaagggcag gatcagccgc atcgtcgtgc cggcgttcgg cttgctgatc
     1081 accaacaggt cgccgcccgc gtcgcgcaac agctcccggg cttgaaaagc accgatgccg
     1141 tgaccgtcgc ccttggtgct gcgcagcggc cggaacagtt catcgcgaac gaactccggt
     1201 gccatgccgg gcccttgatc gataatatcc accacgacgc catgcgcttc catccgcaga
     1261 tcgatttcga ccgcggcgtg cgtatccgag gcctcgaggg cattgttcag cagatgggtg
     1321 acaacggcat cgaacgcatc cggatcgatc gccgcaccgg cactgaagcc gtggtcgtga
     1381 aagcggattt cgcgctggct tgccacgcgg ttggcttcga cgagttcacg cagccgatcc
     1441 gatggcgtga tcaaggcgtg atcgcgttcc tgccggtcgg cctgcaaccg cgacagcagc
     1501 cgtgtgatct tggcaacgga tgcccgcacc gtcgccagca tgtcgcgctg gaactccggg
     1561 ttgtccgcat aaacttcggc attggtcaga agcattgaga gttgcccgga cacgttcttg
     1621 atgtcatgga tgacgaaggc gaagcgctgg ctgtattcgc gaagctgcct tgtctgtgtc
     1681 agcacctgca tggcacgttg ttccgccacg cggctcgcga tttcgcgacc gaccactcgt
     1741 agcaggtcgt aagcctcccg gtccagtttg aaatgcgctc gggagcgggc gagaacgacg
     1801 aaaccgatca ggccgccgaa atggtttaac ggcactacga cccaggaccg caccagttca
     1861 ggcagccatt tttcggcgtc tgcatgctcg tccagatgaa cgatccagtc gccgtcccga
     1921 aacagcgcaa caagcggatg gccgggagga accggtgcag taaccgacgg catgttccag
     1981 gaaccagccc actggaacgc gacatcgctg ggcgggcgca ggaacaaagc ccctgccgga
     2041 ctatccacga cctcggcagc ggcgcggata gctcgtttgt gcaaagccac gaacgcttca
     2101 ggagcggtca atgcgtcgat gcaacgcatc cattcacgac ggtagttgta acggttgctg
     2161 aagaaattct ccaccaccat cgacctgatc cgggaccggg tcgatcccga ggtc
//
LOCUS       DASWQN010001164         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1037582, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001164 DASWQN010000000
VERSION     DASWQN010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037582"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1063
                     /locus_tag="VGC82_19835"
     CDS             167..1063
                     /locus_tag="VGC82_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0425578.1"
                     /translation="MSATTADRRIAVAAARKNSLYRSRMWALWGSYIALGVFVIMFLV
                     PPFYTLMTSLKSSAEISAQTGSPWLVHHPTLENFWYLITYPNFQRYYLNSIIVTVLTV
                     VLSMVISVMAAFSLSRMGFWGSQTLSTGVFLTYLVPPSLLFIPLFQIIGQLGLINNFW
                     CLVILYPTLAVPFCTWIMIGYFSSIPKELDEAALIDGAGYLQMLWKIFIPVAMPGIIA
                     ATIFAFTVAWGAFLYPLAYLYTSNQMVLTVGIISDLIRGDVFQWGKIMAACLLASLPP
                     IIIYAFLMDYYVAGLTAGATKG"
     gene            1132..2235
                     /gene="ugpC"
                     /locus_tag="VGC82_19840"
     CDS             1132..2235
                     /gene="ugpC"
                     /locus_tag="VGC82_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY0425579.1"
                     /translation="MAEVSVRKVVKEYDAGVQAVKGIDLDIADHEFVVLVGPSGCGKS
                     TTLRMIAGLEEITSGEIWIDGDVVNDVPPRDRDIAMVFQNYALYPHMSVFDNMAFGLK
                     LRKFAKDEIRRRVDDAARILDIVPLLDRKPRALSGGQRQRVAMGRAIVRNPKVFLFDE
                     PLSNLDAKLRVQMRTEIKKVHQTIPTTTVYVTHDQIEAMTLADRVVVMNHGVIEQVGT
                     PNDLYHHPATRFVAGFIGSPAMNFLPCRVQDGANGGLTVRVTDAVTVPIPAARADRYA
                     PYKGKEMVLGIRPEHLFEYHEQGKPGWSRVDLPVDVVEPMGMETMVHFFVDGAPVCAR
                     VDPATHAAPGEMMPLTMDLNQMHLMEAASGRVV"
BASE COUNT          449 a          734 c          751 g          494 t
ORIGIN      
        1 cgcaggacat gacgcatgtg ttcgcgacct acgcgttctc ggtcggcatc cagtccggtg
       61 atattccgct gggtgcggcg gacagcctgt tcatgttccc gattctggcc ttcgcggcat
      121 tcttcgtgct gcgcggcgtg acgcagcgca ccaaggagat cgcgacatga gcgccacaac
      181 cgccgaccgc cgcatcgccg ttgccgcggc ccggaagaac agcctgtacc gaagccggat
      241 gtgggcgctt tggggcagct acatcgccct gggcgtgttc gtcatcatgt tcctggtgcc
      301 gccgttctac acgctgatga ccagcctgaa atcgtcggcg gaaatatccg cacagaccgg
      361 cagcccgtgg ctggtgcatc acccgacgct ggagaatttc tggtacctga tcacctaccc
      421 gaatttccag cggtactacc tgaactcgat catcgtcacg gtcctgacgg tcgtgctgtc
      481 gatggtgatc tcggtgatgg cggcgttcag cctgtcacgc atgggtttct ggggcagcca
      541 gacgctgtcc accggcgtct tcctgaccta tctcgtgccg ccgtcgctgt tgttcatccc
      601 gctgttccag atcatcggcc agcttggatt gatcaacaat ttttggtgcc tggtgatcct
      661 gtatccgact ctggcggtgc cgttctgcac ctggattatg atcggctatt tcagctccat
      721 tccaaaggaa ctagatgaag cggccctgat cgacggcgcc ggctatctgc agatgctgtg
      781 gaagatcttc atcccggttg cgatgcccgg catcatcgcg gcgacgatct tcgcgttcac
      841 cgtcgcctgg ggggcctttc tttatccgct ggcttatctc tatacgtcga accagatggt
      901 gctgaccgtc gggatcatct ccgatctgat ccgcggcgat gtgttccagt ggggcaagat
      961 catggcggcg tgcctgctgg cctcgttgcc gccgatcatc atctacgcgt tcctgatgga
     1021 ctattatgtt gcgggcctga ccgccggcgc caccaaaggc tgaacccaca aaaatcataa
     1081 atggccggat cgaacccggc catgaaccca agtcgaggaa acgaagcaac catggccgaa
     1141 gtgtccgtcc gcaaagtcgt caaggaatat gatgccggcg ttcaagcggt gaaaggcatc
     1201 gacctggata tcgccgacca tgagttcgtg gtgctggtcg gtccatccgg ttgcggcaag
     1261 tcaaccacgt tgcgcatgat cgccgggctg gaggaaatca cctccggcga gatctggatc
     1321 gacggtgacg tcgtcaacga cgttccgcca cgcgatcgcg acatcgccat ggtgttccag
     1381 aactacgcgc tgtatccgca catgagcgtc ttcgacaaca tggcgttcgg gctgaaactg
     1441 cggaagttcg ccaaggatga gatccggcgc cgcgttgacg atgccgcccg catcctcgac
     1501 atcgttccgt tgctggaccg caaaccgcgg gcattatcgg gcggccagcg gcagcgcgtg
     1561 gcgatgggac gggcgatcgt gcggaacccg aaggtgttcc tgttcgatga gccgctgtcc
     1621 aacctcgatg ccaagctgcg cgtgcaaatg cggaccgaaa tcaagaaagt ccaccaaacc
     1681 atcccgacca ccaccgtcta tgtgacccac gaccagatcg aggcgatgac attggccgac
     1741 cgcgtcgtgg tgatgaacca cggtgtcatc gagcaggtcg ggacgccgaa cgacctgtat
     1801 catcatccgg ccacgcgttt cgtcgccggg ttcatcgggt cgcctgcgat gaatttcctg
     1861 ccgtgccgcg tgcaggacgg tgcgaatggc ggcttgacgg tgcgtgtgac ggacgccgtc
     1921 acggtgccga tcccggctgc ccgggctgac cgctacgctc cctacaaggg gaaggaaatg
     1981 gtgctgggca ttcggccgga gcatctgttc gagtaccatg aacagggcaa gcccggctgg
     2041 tcgcgggtcg atctaccggt ggacgttgtc gagccgatgg gcatggaaac catggttcat
     2101 ttctttgttg acggcgcgcc tgtctgcgcc cgcgtcgatc cggcgacgca cgccgccccg
     2161 ggggaaatga tgccgctgac catggacctg aatcagatgc acctgatgga agccgccagc
     2221 ggcagggtgg tctgagcggg agctggggag gcctgcgagt gctgatagtg ttctcgcggt
     2281 gcccagaccc agggcagcga tcggttgctt ttggatctta agccgcttcg tcgtcgccat
     2341 gcacgcctga gccaaagcag aaacgccagc gccgaaacgc ctgaagggcc caggacttgg
     2401 gtctaaacgg gcccaggtga gcgagtac
//
LOCUS       DASWQN010001165         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1434594, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001165 DASWQN010000000
VERSION     DASWQN010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434594"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1475
                     /locus_tag="VGC82_19845"
     CDS             174..1475
                     /locus_tag="VGC82_19845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0425580.1"
                     /translation="MNAGIEKSAMRKVYLRLLPLTMVMYFLCYIDRINVSFAALTMNK
                     DIGLTAAAYGFSSTAFYVGYVLFEVPSNLVMDKVGARLWLARIMVSWGLISAATAAVV
                     GPTSFLLVRFLLGAGEAGLFPGTILLFTYWFPDAYRGRIIGFFTLALPVSVALGAPIS
                     TAILSMDGIFGVKGWQWIYILEAVPTVIVGIFVLVAMSDRPESAKWLSGPEREWLAAT
                     LQTERLAVEAGGKFSIVGSMTNPKVLLLSVNYLGIVTASLGLLLFIPQIIQSLGASTM
                     NTGYATSLAYICGAVAMILSGWLSDRLGDRRWLLCGTCMMATVGLVIAGATMGSWWSL
                     AGMCLATAGFYGTKGPFWAMPTMILTGAAAASGIAFINAFGNVGGAVGPALVGWLKDV
                     TGSYAGGLYGLAAFTGVGAVIAAVGLRIPRRVGAGAVAPAE"
BASE COUNT          341 a          543 c          627 g          413 t
ORIGIN      
        1 gataggcacc tgcgatgggt ttgtcaccaa gctgcagtgc tgaacgggcg ggcgttcagc
       61 accgatgtgc aatgactggt attaaaaacg ccataaggct cggcttatgg gtcgacggag
      121 ggaacctgag gtcgaccaag gaatttacct tgaagtcacc agggaggaga cagatgaacg
      181 ccgggatcga aaaatcggcg atgcgcaagg tgtatctgcg tctgctgccg ctgacgatgg
      241 tgatgtattt tctctgctac atcgaccgca tcaatgtcag tttcgcggcg ctgacgatga
      301 acaaggacat cgggctgaca gcggcggcgt acggtttcag ctcaacagcc ttttatgtcg
      361 gctacgtcct cttcgaggtt cctagcaacc tggtcatgga caaggttggc gcccggttat
      421 ggctggccag gatcatggta agttggggtc tcatttcagc cgctaccgcc gccgtggttg
      481 gaccgaccag cttcttattg gttcgcttcc tcctgggtgc tggcgaagcc gggctgttcc
      541 ccggcacgat cctgctgttc acctattggt ttcccgacgc ttaccgcggc cggatcattg
      601 ggttcttcac gctggcactt cccgtctcgg tcgcccttgg tgctcccatc tccaccgcca
      661 tcctaagtat ggacggtatc tttggcgtca aagggtggca gtggatctac attctggagg
      721 ctgtgccgac ggtgatcgtt ggcatcttcg tgctcgtcgc gatgagcgat aggccggaga
      781 gtgcgaaatg gctcagcggg cccgagcgag aatggctcgc cgccacgctg caaactgaac
      841 gtcttgcggt cgaggccggc ggcaagttca gtatcgtggg aagcatgacc aacccgaagg
      901 tgttgttgct gtcggtgaac tacctgggca tcgtgaccgc cagtctaggc ctgctgctgt
      961 tcattccgca gatcatccag tcgttgggcg cctcgaccat gaacaccggt tatgccacca
     1021 gcttggccta tatctgcggg gcggtcgcca tgatcctgtc tggttggctg tcggaccggc
     1081 tgggggaccg ccgctggctg ctgtgtggaa cgtgcatgat ggcgacagta ggccttgtga
     1141 tcgcgggcgc cacgatggga agctggtggt cactggcggg catgtgcctc gccaccgccg
     1201 ggttctatgg gacgaagggg ccgttctggg cgatgccgac catgatcctg actggtgccg
     1261 cggcggcaag cggcatcgcg ttcatcaacg ccttcggcaa cgtgggcggt gccgtaggac
     1321 cggcgctggt gggatggctg aaggacgtga ccggcagtta cgcaggcggc ctctatggcc
     1381 tggcggcgtt cactggcgtt ggcgccgtga tcgccgcggt cggactacgt atcccacgcc
     1441 gcgtcggcgc gggggctgtg gcgccggctg agtaaggtag caccttccga gccggaagcg
     1501 ttgctcgggt gcagcgagag agggaacggc gtccgatacg gctccagctg gacgaagacc
     1561 ttctccagtt ttctcctcgg cgccattgta tgcgcggata tccaaggtgc tgctcgaaaa
     1621 tcctgccgca ctatctcagc tggcgccgac ctctggaagc cttgggtcaa cagatcatac
     1681 ccgaagcggt atctcgaggc tgtcggactg ggaccctatc aacaaacggc gcgataagag
     1741 tcatgcacaa gatctatcgc ttggtgcgct cgatttcttg gccacccggg ggcatctgcg
     1801 ggcatcatcc gccagctcag gaaacggtcg cgaaacggga agccgtgcgc tcagccgtaa
     1861 tcgcggagca gttttgcgcc tatcgatgca tcatcaccgg caaggtcgcg tgctccagga
     1921 tgtg
//
LOCUS       DASWQN010001166         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1169303, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001166 DASWQN010000000
VERSION     DASWQN010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169303"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1443)
                     /gene="thrC"
                     /locus_tag="VGC82_19850"
     CDS             complement(37..1443)
                     /gene="thrC"
                     /locus_tag="VGC82_19850"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY0425581.1"
                     /translation="MRYISTRGQAPARDFTDVLLAGLAEDGGLFMPESWPHFSPSDWR
                     AMRGLPYAELAARVMQPFMAGSIPFDVLRSICRDAYAGFRHPAIVPLVQLETGLFVQE
                     LFHGPTLAFKDIAMQVLGRLFDYVLTQRDRRVTIVGATSGDTGSAAIEACAGRDRVDI
                     MILHPHGRTSLVQRRQMTTVHAGNVGNVAIEGTFDDCQDLVKAMFADAGFRSEVRLSA
                     VNSINWARIAAQVSYYVAAALALGAPDRDVAFSVPTGNFGNVLAAWAARRMGLPVARL
                     IVGSNRNDILARFLDANDMSMVPVEPSLSPSMDIQISSNFERLLFELLDRDPVATGRT
                     MLDFRKTGRMTIADPVWHRARGLFHGFRLDDPGTEAEIRRLDAACGYLADPHTAIGIA
                     AARALPCAGGVPVVAMATAHPAKFPDAMQAATGQRPALPPRLADLLQREERFTVLPND
                     LSAVEAAVRALVGRNSPL"
     gene            complement(1461..2447)
                     /locus_tag="VGC82_19855"
     CDS             complement(1461..2447)
                     /locus_tag="VGC82_19855"
                     /inference="COORDINATES: protein motif:HMM:NF006564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HEY0425582.1"
                     /translation="MTASSEAQFAAFVATLGRGPGRSRALTREEAHTAFGMVLRNEAD
                     PHQVGAFLMLLRFRGEDAEEITGMVEAARAHATLGRVDLGVDLDWPSYGAGRTRGAPW
                     FLLAALALARSGVTVLMHGSNDFSSGIAVEDAMAAIGLRPDPDLAAAKATLQGSRFAY
                     LPLRSLAPALHRLLDMRRLFGLRSPVNTAVRLLNPADAPYGVDGVFHPPYIDVHLGVA
                     ERMRRRRLLVLKGGGGEAERVPMKPTTATVWDIEAGRQEMMLPAVDRLRPHPAASDTP
                     DSFAAVWAGQATPETPLATIQATIALALLAIGRTNDPVEAMRHAVTIWAERR"
     gene            2689..>2907
                     /locus_tag="VGC82_19860"
     CDS             2689..>2907
                     /locus_tag="VGC82_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0425583.1"
                     /translation="MAETEVQVLGGLLRQWRRRRRMSQLDLACDAEISTRHLSFLETG
                     RSRPSRQMVLHLAGCLDVPLRERNVLLTA"
BASE COUNT          530 a          979 c          960 g          438 t
ORIGIN      
        1 gcgctcggga taaattcacc gcgctgtcag ccaatcctac aacggcgaat tacgtcccac
       61 caatgcccga accgccgctt ccacggccga aagatcgttc ggcagcacgg taaaccgctc
      121 ctcccgctgc agcaggtcgg ccagtcgcgg cggcagcgca ggacgctgtc cggtcgccgc
      181 ttgcatcgcg tccgggaatt tcgccggatg cgcggtcgcc atcgcgacaa ccggcacccc
      241 acccgcgcac ggcagcgccc gagccgccgc gatcccgatg gccgtatgcg gatcggcaag
      301 ataaccgcag gctgcgtcca gccggcggat ctccgcctcg gttcccggat cgtccagccg
      361 gaagccgtgg aacaggccgc gtgcgcgatg ccagacaggg tcggcgatcg tcatccgccc
      421 ggtcttgcgg aaatccagca tcgttcgtcc cgtcgccacg ggatcgcgat ccagcagctc
      481 gaacaacagg cgttcgaagt tggagctgat ctggatatcc atgctcggtg acaacgacgg
      541 ctccaccggg accatcgaca tgtcgttcgc atccaggaac cgcgccagga tgtcgttgcg
      601 gttcgaaccg acgatcaacc gcgccaccgg cagccccatc cgccgggcgg cccaggcggc
      661 caggacattg ccgaagttcc ccgtcggaac gctgaacgcg acatcccggt ccggcgcgcc
      721 caacgccaaa gccgccgcga cataatacga aacctgcgcc gcgatgcgcg cccagttgat
      781 ggagttcacc gccgacagcc ggacctccga ccggaagccc gcatcggcga acatcgcctt
      841 caccaggtcc tggcagtcgt cgaaggtgcc ctcgatcgcg acgttgccga cgttgcccgc
      901 atggacggtg gtcatctgcc ggcgctgcac caggctggtg cggccatgcg gatgcaggat
      961 catgatatcg acccggtcgc gcccggcgca ggcctcgatc gcggcggacc cggtgtcgcc
     1021 ggaggtcgcg ccgacgatgg tcacccgccg gtcgcgctgg gtcagcacgt agtcgaacag
     1081 gcgccccagc acctgcatcg cgatgtcctt gaacgccagg gtcgggccgt ggaacagctc
     1141 ctgcacgaac agcccggttt cgagttgcac cagcggcaca atggcgggat ggcggaaccc
     1201 ggcataggcg tcccggcaga tcgaccgcaa aacgtcgaac gggatcgatc cggccatgaa
     1261 cggctgcatg acccttgcgg ccagctccgc atagggcagg ccgcgcatcg ctcgccaatc
     1321 ggacgggctg aaatgcggcc agctctcggg catgaacagc ccgccgtcct cggcgaggcc
     1381 ggccagcaga acgtcggtga aatcgcgggc aggcgcctgg ccgcgtgtgg aaatgtagcg
     1441 catgcccggg ttctacgcct ttaccggcgc tccgcccaga tcgtaacggc atggcgcatc
     1501 gcttcaaccg gatcgtttgt ccggccgatg gccaggagag cgagcgcgat ggtcgcctgg
     1561 atcgtcgcta aaggtgtttc gggcgtggct tgcccggccc agacggcggc gaaggaatcc
     1621 ggcgtgtccg atgccgccgg atgcggccga agtctgtcca cggccggcaa catcatctct
     1681 tgccgccccg cctcgatgtc ccagacggtt gccgtggtgg gtttcatagg cacgcgttcc
     1741 gcctctccgc cgccgccctt cagcaccagc agccggcggc ggcgcatgcg ttcggcaacg
     1801 ccgagatgca catcgatgta gggcggatgg aacacgccat cgacgccgta cggcgcgtcc
     1861 gccggattga gcagccgaac agcggtgttc accggcgagc ggaggccgaa caggcgccgc
     1921 atgtccagca gccggtgcag cgccggcgcc aggctgcgca acggcaggta ggcgaagcgg
     1981 ctaccctgaa gcgtggcttt ggcggcggcc agatcaggat ccggccgcaa tccaatggca
     2041 gccatcgcgt cctctacggc gatgccggag gaaaaatcgt tcgagccgtg catcaacacc
     2101 gtgactccgg aacgagccag cgccagggct gccagcaaga accaaggggc gcctcgggtt
     2161 cggccggcgc cgtaggacgg ccaatcgaga tcgaccccga gatcgacccg gccaagcgtc
     2221 gcgtgggcgc gcgccgcttc gaccatgccg gtgatttcct cggcgtcctc gccacggaaa
     2281 cgcagcaaca tcaggaaggc accgacctga tgcggatcgg cctcgttgcg caggaccatg
     2341 ccgaaggcgg tgtgcgcctc ctcccgcgtc agggcgcgcg accggccggg cccgcgaccc
     2401 agtgtggcga cgaaggcggc aaattgggct tcggacgatg cggtcatgga tgtcagcgta
     2461 tagacccttc ggttcgtgtt tggtacaggt ccggcgaagt ctcatggcac agcggcccgg
     2521 gcaccgcgac gataagtcgg cacgtgcgcc gtggttgcgg cactcgcgat gccgagcagg
     2581 tcggaggttg gaattaacgc ctctgccact cattccgggt tatgatgttc catccgaata
     2641 cgcaacaccc gggcgaaaca gactggccgg aaggaaggaa ctcgaagggt ggcagagaca
     2701 gaagttcagg tcctgggtgg gttgctgcgc caatggcggc gcaggcggcg catgagccaa
     2761 ctcgatctgg catgcgatgc ggaaatctcc accagacatc ttagctttct tgaaaccggc
     2821 cgctccaggc caagccggca gatggtgctg cacctggcgg gatgtctgga tgtgccgctg
     2881 cgcgaaagga acgtcctgct gaccgca
//
LOCUS       DASWQN010001167         1923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1697757, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001167 DASWQN010000000
VERSION     DASWQN010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697757"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..346
                     /locus_tag="VGC82_19865"
     CDS             155..346
                     /locus_tag="VGC82_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEY0425584.1"
                     /translation="MHGFFTILIGIGMLATLGVLVAGFIGLIRDGDARRSNKLMQWRV
                     LLQGVTILLLVIFMSLLRS"
     gene            501..1250
                     /locus_tag="VGC82_19870"
     CDS             501..1250
                     /locus_tag="VGC82_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY0425585.1"
                     /translation="MKIIVPVKRVVDYNVKVRVKSDGSGIETAGVKMSMNPFDEIAVE
                     EAVRLKEKGVATEIIAVSMGVPQCAETIRTALAMGADRGILVESDADLQPLAVAKLLK
                     AIVDKEQPRIVILGKQAIDDDMSATGQMLAALLGWSQGTFASKLTVEGDTLMVTREVD
                     GGLETVALTLPSVVTTDLRLNEPRYASLPNIMKARKKPIVNMKPADLGVDVTPRLTVL
                     KVSEPPKRKAGQKVGSVAELVNKLRTEAKVI"
     gene            1253..>1923
                     /locus_tag="VGC82_19875"
     CDS             1253..>1923
                     /locus_tag="VGC82_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0425586.1"
                     /translation="MTSLVLLEFDETGIKQPSRSAVAAATALGEVHGLVAGINLGGAS
                     AAAAKLPGVTKVLVADNPALDHLLAEPIAALLLALAPAYTHMLAATTAVGKNVMPRVA
                     ALLDLQPISDIAGVEDADTFIRPIYAGNGLATVKSSDSKKIITVRAASFDPVAAEGGN
                     ASTENVTVGDMPGLSKFVSAELSKSERPELTAARVVISGGRGMQNGENFKLIEPIADK
                     LGAAVG"
BASE COUNT          378 a          597 c          599 g          349 t
ORIGIN      
        1 aactttatga tctgggaaat cgggatggtt ctccccacgc ataagcgccg acgttcatct
       61 gccgctccat gacttcgccg gacaaccaga tttcggatgg atcgctcaac ccacccacct
      121 ccacaattgg cacccagcac cccattctaa agcgatgcac ggttttttca ctatcctcat
      181 cgggatcggg atgctcgcaa ctttgggggt gctggtcgcg ggattcatcg gcctcatccg
      241 ggatggtgac gcgcggcggt ccaacaagtt gatgcaatgg cgggtactcc tccagggcgt
      301 tacgatcttg ctgctggtga ttttcatgag cctgctgcga tcctgatccg gcggcggcct
      361 ggctgcgcgt ttgacacgca taaaccccgc ccatatggtc cggcgccctc tcgcaggtgc
      421 gaacagacgc tcggaaccgg gacggcgccg agcgtttaag tgtgtgtccc caacgtcccc
      481 aggaagcagc acggtttccc atgaaaatca tcgttcccgt caaacgggtt gttgactaca
      541 acgtgaaggt tcgcgtgaag tccgacggct ccggcatcga gaccgccggc gtcaaaatgt
      601 cgatgaaccc gttcgatgag atcgcggtcg aggaggccgt gcgccttaag gaaaagggcg
      661 tcgcgaccga gatcattgcc gtttccatgg gtgttccgca atgcgcggaa accattcgca
      721 ctgcgctcgc catgggcgcc gaccgcggca tcctggtgga aagcgatgcc gacctgcagc
      781 cgctggcggt cgccaagctg ctgaaggcga tcgtggacaa ggaacagccg cggatcgtca
      841 tcctcggcaa gcaggcgatc gacgacgaca tgagcgccac gggccagatg ctggcagcct
      901 tgctcggctg gtcgcagggc accttcgcca gcaagctcac tgtcgagggc gacaccctga
      961 tggtgacacg cgaggtcgat gggggactgg aaacggtggc tctgaccctg ccgtccgtcg
     1021 tcaccaccga cctgcggctg aacgagccgc gctatgcgtc gctgccaaac atcatgaagg
     1081 cgcggaaaaa accgatcgtc aacatgaagc cggccgatct cggcgtcgat gtaacgcctc
     1141 gtttgaccgt gctgaaggtg tccgaaccac cgaagcgcaa ggccggccag aaggttggtt
     1201 cggtggcgga actcgtgaac aaactccgca cagaagcgaa ggtgatctga cgatgacgag
     1261 cctggttctc ctggaattcg acgaaacggg catcaagcag ccgtcgcgca gcgcggtcgc
     1321 cgcagccacc gccctgggcg aggtgcatgg actggtcgcg ggcataaatc ttggtggcgc
     1381 ctccgccgcc gcggcaaaac tgccgggcgt gaccaaggta ttggtcgcgg ataatcccgc
     1441 cctggatcat ctgttggcgg agccgattgc agcgctgctg ctcgcgctgg caccggcata
     1501 cacgcacatg ctcgcggcga ccacggcagt cggcaagaac gtcatgccgc gcgttgcggc
     1561 gctgctggac ctgcagccga tcagcgacat cgccggcgtc gaggacgccg acaccttcat
     1621 ccgtccgatc tatgccggca atggccttgc cacggtgaag tcgtcggatt ccaagaagat
     1681 catcactgtc cgcgcggcca gtttcgaccc ggtggcagcc gaaggtggca acgcttcgac
     1741 cgagaacgtg accgtcggcg acatgcccgg cctgtcgaag ttcgtgtccg ccgaactgtc
     1801 caagagcgag cggcccgaac tgaccgccgc ccgcgtggtg atttccggcg gccgtggcat
     1861 gcagaacggc gaaaacttca agcttatcga acccattgct gataagcttg gtgctgctgt
     1921 cgg
//
LOCUS       DASWQN010001168         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1434880, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001168 DASWQN010000000
VERSION     DASWQN010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434880"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..732)
                     /gene="petA"
                     /locus_tag="VGC82_19880"
     CDS             complement(169..732)
                     /gene="petA"
                     /locus_tag="VGC82_19880"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEY0425587.1"
                     /translation="MADSSAAATHHAASGDVTKRDLLQLVAMAGAAIGAGAIAWPLID
                     SMNPSKDVLALSSVEVDLTPIVEGQGITIVWQGKPIFVRHRTPAETKEAQDVPLGQLI
                     EPQADLARVKPGHAQWIILIGICTHLGCVPLGNKPSDPRGDYGGWFCPCHGSQYDTSG
                     RVRHGPAPANLALPPYAFASDTKIKVG"
     gene            924..>1659
                     /locus_tag="VGC82_19885"
     CDS             924..>1659
                     /locus_tag="VGC82_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEY0425588.1"
                     /translation="MVENPVTIPHEKLKPAARAWFEQLRDRICTTFEAIEDAHTGPLS
                     DRPPGRFERSAWTRPTEDGSTGGGGVTSLMKGRVFEKVGVNVSTVWGEFSPDFRAQIP
                     GAAEDPHFWASGISLVAHMQSPLVPAAHFNTRMLVTTKGWFGGGGDLTPMRLGTEQAK
                     QDAADFHASFRAACDRHDPGYYATFKAWCDRYFYLPHRQEPRGAGGIFFDHHSTGDTD
                     ADFAFARDVGEAFLDSYPRIVRRRMGQ"
BASE COUNT          318 a          512 c          519 g          310 t
ORIGIN      
        1 cagaagtaat taaaattccg cggtgtgggg aaaactccat actccttttg caccatcgtg
       61 aagatgggca ggcgggtatc gatccacttt accagcggat tggcgaattc actcttgtgc
      121 aggccggcca tgtttatttc cctgtagcgg gtcggagggc tgaacggatc agccgacctt
      181 gatcttggtg tcggatgcga atgcgtaagg gggcaaggca agattcgctg gcgcaggtcc
      241 gtggcgcaca cgtccggagg tatcgtactg gctaccgtgg caggggcaga accatccgcc
      301 atagtcacca cgcgggtcgg atggcttatt gcccagcggc acgcagccca ggtgggtgca
      361 gatgccgatc aggatgatcc actgggcgtg acccggcttg actcgagcca agtccgcctg
      421 tggttcgatc aactggccca ggggaacatc ctgggcttcc ttggtttccg caggcgtccg
      481 gtggcgcacg aaaatcggct tgccctgcca gacaatggtg atgccctgac cctcgacgat
      541 cggcgtaagg tccacctcga ccgacgacaa cgccagcacg tccttggacg ggttcatgga
      601 gtcaatcagc ggccacgcga tggcgccggc accgatcgcc gcgccggcca tggccacaag
      661 ttggagcaaa tcccgcttgg tcacatcgcc cgaggcggcg tgatgggtgg cagcagcgga
      721 cgagtcggcc atgtttttcc tcgagttacc gtttcaacaa ggacgcggcg cggttacccg
      781 cgggccaatc tttcgatcga tccggagaac ccgccgggtt gccccggaag cagcgtttgg
      841 cgcaatctat gcactggcct cgacccctac aacaagctgg cagcagcggg ttatcccgac
      901 tgtcacgcag gaacaagccc ccgatggtgg aaaatcccgt taccataccg catgagaaac
      961 tcaaaccagc ggcgcgggcg tggttcgagc agttgcgcga tcgcatctgc accacattcg
     1021 aagcgatcga ggacgcccat actggcccgc tcagcgaccg tccgcccggc cggttcgagc
     1081 gctcggcgtg gacgcggcct accgaagacg ggtcaaccgg cggcggcggc gtcacgagcc
     1141 tgatgaaagg ccgcgtattc gaaaaagtcg gggtgaacgt ttccaccgtc tggggcgaat
     1201 tcagtccgga tttccgtgcc cagatcccgg gcgcggccga ggatcctcac ttctgggcca
     1261 gcggcatcag tctcgtggca cacatgcaaa gcccgctggt gccagccgcg cattttaaca
     1321 cccgcatgtt ggttactacg aaaggctggt tcggcggcgg cggggacctg acgccgatgc
     1381 gcctcggcac ggaacaggca aaacaggacg cggcggattt tcatgctagc ttccgcgccg
     1441 cctgcgaccg ccatgacccc ggctattatg caacgttcaa ggcttggtgc gaccggtact
     1501 tctatcttcc gcaccgccag gagccgcgtg gcgccggcgg catatttttc gatcatcata
     1561 gcaccggcga cactgatgcg gacttcgcct tcgcacgcga tgtgggcgag gcttttctgg
     1621 actcatatcc caggatcgtt cgtcgccgca tgggccagc
//
LOCUS       DASWQN010001169         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1565178, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001169 DASWQN010000000
VERSION     DASWQN010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565178"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VGC82_19890"
     CDS             <1..251
                     /locus_tag="VGC82_19890"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEY0425589.1"
                     /translation="LWEARLTPQDRQRWQPHVTIQNKADTIMAKALYQRLQADFSPYQ
                     VVAAGLGLWIYRNGPWEAIGSFPFAAKTHGLAASAMTT"
     gene            complement(244..1242)
                     /locus_tag="VGC82_19895"
     CDS             complement(244..1242)
                     /locus_tag="VGC82_19895"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0425590.1"
                     /translation="MPGSTRNAGLDGLRGIAALSVALGHCVLQVTGIGLWGTTLHNFH
                     SMPAADIGMRLLSALFPSDAAVTVFFVLSGHVLWGSFKRKQLWFFKDLPDYACARIYR
                     LFPLVIVTAMPLGLLSGAPASLLVRNMLLLDKSMNGVLWSLQVEVVASFALFALWGLT
                     RGSTWKLLLGLVLAVAATQYAMGYSAIVFFPAFILGASASLIPPRLVRRPWIIAAGTV
                     VLVFTNVLLGHNGITRFFEMAGATVLVGAVASGELRFLRARLPLFLGTISYPFYLMHV
                     IGLLAADPILIALGPLPPLVAIAARAAVSIAFTVPVAWLLHVFVEDPVLRARPRIR"
     gene            complement(1319..1585)
                     /locus_tag="VGC82_19900"
     CDS             complement(1319..1585)
                     /locus_tag="VGC82_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GapR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY0425591.1"
                     /translation="MAKAVSEEEIKSGGVAVDRLRSLVERIERLEEERKALGSDIKDI
                     YAEAKSAGFDPKVLRQLIRIRKQEAAEVEEQETMLDIYRRALGM"
     gene            complement(1713..>2255)
                     /locus_tag="VGC82_19905"
     CDS             complement(1713..>2255)
                     /locus_tag="VGC82_19905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425592.1"
                     /translation="TWQILEAQGFTRFAEGRTVAIPVLSPPRGAAKVGGAPKVWGAPK
                     VWAVRPDMPGQISAGPDLEQGEITLLLDHADFGCLSLVCETAEGRQPFVFGLHRRSGI
                     IPVAHLVYCRDLEDFVRLARPIGQHLLRRGYGFVILASDGPIEGLVGRYAGGRPRYFK
                     GPDRPRIGDVAYSEQVIFGF"
BASE COUNT          471 a          740 c          678 g          366 t
ORIGIN      
        1 agctctggga agcccgcctg acaccacagg atcgccagcg gtggcaaccc catgtgacca
       61 tccagaacaa ggccgatacc atcatggcta aagcgctgta tcagcgactg caagccgatt
      121 tctcgccgta ccaggtcgtt gcggccggcc tgggcctgtg gatctaccga aacggcccat
      181 gggaagcgat cggcagtttt ccatttgctg ccaagacaca cggccttgct gcttccgcga
      241 tgactacctg atccgaggcc gcgcgcgcag cactggatcc tccacgaaaa cgtgcagaag
      301 ccacgccacc gggacggtga acgcgatcga caccgcggca cgcgctgcga tcgccaccaa
      361 aggcggcaag gggcccaatg cgatcaggat cggatcggcg gccagcagtc cgataacgtg
      421 catcaggtag aacggatagc taatggttcc caggaataac ggcaagcggg cccgcaggaa
      481 gcgaagctcg ccgctcgcca ccgcgcctac caacaccgtt gcacccgcca tctcgaagaa
      541 gcgggtgatg ccgttatgac cgagcaggac gttggtgaag accaggacca cggtcccggc
      601 ggcgatgatc catggccggc gcaccagccg cggcgggatc aaggacgccg atgcgcccag
      661 gataaaggcc ggaaagaata cgattgcgct atagcccatt gcgtactggg tcgccgccac
      721 cgcgagtaca agacccagca gcagcttcca tgtggaaccg cgcgtcagcc cccagagcgc
      781 gaacaacgca aagctggcca cgacctctac ctgcagcgac cagagaacac cgttcatgct
      841 cttgtcgagc agcaacatgt ttctgacaag tagtgaggcg ggagcgccgc tcagcaaccc
      901 cagcggcatc gccgtgacga tcaccagcgg aaacagccgg tatatccggg cgcaggcgta
      961 gtccggcaga tccttgaaga accaaagctg cttccgtttg aacgatcccc agagcacatg
     1021 gccgctgagg acgaaaaaca ccgtcacggc ggcgtcgctc ggaaacagcg ccgacagcag
     1081 ccgcatcccg atatcggccg ccggcatcga gtggaaattg tgtaaagtgg taccccagag
     1141 gccgatcccg gtgacctgca gcacgcaatg gccaagtgct acggaaagcg ccgcgatacc
     1201 ccgcaggccg tcaagcccgg cgttcctggt ggacccgggc atcgtcggct gaccttcagg
     1261 tttggctccg cctattgcgg ccgaaccagg tcggacagta tttggaagat ggtcgatgct
     1321 acatccccag cgcgcggcgg tagatgtcga gcatcgtctc ctgctcctcg acctccgccg
     1381 cttcctgttt acggattcga atgagctggc gcagcacctt ggggtcgaag ccggcggatt
     1441 tcgcctcggc gtagatgtcc ttgatgtcgc tgccgagggc cttgcgttct tcctcaagcc
     1501 tctcgatacg ctcgacgagg ctgcgaagac gatcgacggc gacgccgccg gacttgattt
     1561 cttcttcgct tacggctttg gccattccgt cgtatctccg ctcaggcagg gccggcgggg
     1621 atagctttgg gtgccctcgc ggtcaatccc aaacaggtga tcgcgacgcc ccaagccagc
     1681 ggtggcgggt tcagccgctt tcgaacaggg cctcaaaatc cgaatatgac ctgttcggaa
     1741 taggcgacgt ctccgatccg aggccggtcg gggcctttga agtaccgggg ccgtcctccg
     1801 gcgtatcgtc cgaccagtcc ttcgatgggg ccgtcggagg ccagaatgac gaaaccgtac
     1861 ccccggcgaa ggagatgctg gccgataggc cttgccagcc gaacgaaatc ctccagatcg
     1921 cggcaataaa ccaggtgcgc gaccggaata atcccggaac ggcggtgcag cccgaacacg
     1981 aacggctgcc gtccctcggc ggtctcgcag accagactca ggcatccgaa atccgcatga
     2041 tccaacagca gcgtgatctc accctgctcc agatccggcc cggcgctgat ctgccccggc
     2101 atgtcaggac ggacagccca gacctttggc gcgccccaga cctttggcgc gcccccaacc
     2161 tttgccgcac cccggggcgg cgacagcaca ggtatcgcaa cggtccgtcc ctcggcgaat
     2221 cgggtgaagc cctgcgcttc aagaatttgc caggt
//
LOCUS       DASWQN010001170         1815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1038254, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001170 DASWQN010000000
VERSION     DASWQN010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038254"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..1624)
                     /locus_tag="VGC82_19910"
     CDS             complement(275..1624)
                     /locus_tag="VGC82_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016913619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEY0425593.1"
                     /translation="MSNFRPTDRRIDYLMPPSVEEWLPQRHLARFVVEMVESLDLRGM
                     SASDRGSGSASYCPAVLLSLLIYGCATGGFSSRKLERATYDSVAFRFIAANDHDTLAT
                     FRRRFLPRIEALCVEVLKLAREMGVLQMGTVALDGTKIHANASRHSALSYEHAGKIEA
                     QLKAEVADLLAKAEAADQADIPDGMSIPEELERREARLAKLAEARAKIEARAKERYDL
                     EQAEHQAKLKAREAKAEATGKMPRGRPPQPPVEGPLPTDQINLTDEESRIMPVAGGGF
                     EQCYNAQAVVAEGSLLVLATDVVQATNDKQPLQPMLSKVEALPDELGKVTTLLADTGY
                     VGAANVSACAAPLIAPLIAMGRQPHYPPLSERFAEAPDAPENPTPVEAMAHRLQTPAG
                     RKLYALRKQTPEPVFGIIKSVIGFRQFLLRGHDAVRGEWSLVTMAWNMKRLFALSPA"
BASE COUNT          318 a          585 c          537 g          375 t
ORIGIN      
        1 gagatgcagt gattttcctg atacaccata gctgccgggt catatcgtcc tgaatatgat
       61 ataacttctt cgtgatcatc tttcgatacg aaagacggtt taatatccgt cctagcagcc
      121 tgtcggactg agatttacgg ctgactgcgc aaaagcggtg cgattgccgt cggattcggc
      181 tctggcggcg attgtcccgc tttcagacgc catcgcggtc gatatcaggc ctctgtaagc
      241 gtgcttcacg caaaaagcgc gatctcccgc ctgatcaggc ggggctgagg gcgaacaacc
      301 gcttcatatt ccacgccatg gtcacaaggc tccattcgcc ccggaccgca tcatgcccgc
      361 gcagtagaaa ttgccgaaag cctatgaccg acttgatgat gccgaacacc ggctccggcg
      421 tctgcttgcg caatgcatac agcttcctgc cagccggggt ttgcagccga tgcgccatgg
      481 cctcaaccgg tgtcggattt tccggcgcgt ccggcgcttc cgcgaaacgt tcgctcaacg
      541 ggggataatg cggctgccgt cccatcgcga tcagcggcgc gatcagcggt gcggcgcagg
      601 ccgaaacatt cgccgcgccg acataccctg tgtctgccag caacgttgtc actttgccca
      661 gttcatcggg cagtgcctcg accttgctca gcatcggctg aagcggctgc ttatcgttgg
      721 tggcctgaac cacgtcggtg gccagcacca gcaggctacc ctcagccacc accgcctgcg
      781 cgttgtaaca ctgctcgaac cccccgcccg ccaccggcat aatgcgcgat tcctcgtcag
      841 tcaggttgat ctggtcggtc ggcaacggac cttccaccgg cggctgcggc ggtctaccac
      901 ggggcatctt gccggtggct tcggccttgg cctcccgcgc cttcagcttc gcctgatgct
      961 cggcctgctc gaggtcgtaa cgctccttgg cacgggcttc gatcttcgcg cgggcctcgg
     1021 cgagtttcgc cagccgcgcc tcccgccgct ccaactcctc cggtatcgac attccgtcgg
     1081 ggatgtcggc ctgatccgcc gcttcggctt ttgccagcaa atcagccact tccgccttga
     1141 gttgcgcctc gatcttgccg gcgtgctcgt aggagagtgc gctgtggcgg ctggcattgg
     1201 cgtggatctt ggtcccatcc agcgccaccg tgcccatttg cagaaccccc atctcgcggg
     1261 ccagtttcag cacctcgacg cacagcgcct cgatccgcgg caggaagcgc cgccggaagg
     1321 ttgcgagggt gtcgtggtcg ttcgcggcga tgaagcggaa ggcaaccgaa tcataggttg
     1381 cccgttccag cttgcggctg gaaaatcccc cggtggcaca gccgtaaatc agcaggctca
     1441 gcagcaccgc cgggcaatac gaagctgatc cggagccgcg atcgctggcg ctcatgccgc
     1501 gcaggtccag gctctcaacc atctccacaa cgaaccgagc cagatgacgc tgcggcagcc
     1561 attcctccac agacggcggc atcaggtagt caatccggcg gtcggtgggg cggaagttgc
     1621 tcatgcgtgt gtccggcggg tgggcgaatc gtcgttgcga ttctaactca gacaccgcgt
     1681 ggcgttaagt ccgacaggct gctagtcggc tcttattgac gcgcacaagg ccaggcgggc
     1741 gatggttcat tatcgggtgg tacaccaccc tcgatcgaga ggcgaggaaa attccggtat
     1801 tttgctgtta attga
//
LOCUS       DASWQN010001171         1983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1303162, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001171 DASWQN010000000
VERSION     DASWQN010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303162"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(328..>1983)
                     /locus_tag="VGC82_19915"
     CDS             complement(328..>1983)
                     /locus_tag="VGC82_19915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425594.1"
                     /translation="YDVKGPFPRQEDSPAWQAGLRPGDRLDLPAMTCRDPQNRRCIDL
                     LAVLGGMGGRQLVKPGRVLRLQILPADGSAVRTVDVPAKLTEPTSLDRGVLLFNEVIA
                     ALFILAAAWLVWTRPGGMTWGFFLYAIWFNSGQNFVFYAFLQERPHLLLAQEAAGAVM
                     QGLGYAGFLLFVLRVPSDQTSPAWNGWRFVLPLVALLFIGLQLLSYANAFGQPSEAVS
                     RTTFLAGFVVDAAAILILLRRRRGQSPQQYQRLRWVIWGCVLGLPAFIVAAILQSTTL
                     WRFIPGVTTVPSDLIALLYALHGLFAWFVFEAVRRPRVVSVSMPLRRISVFGLMLSVP
                     ALFLHQQIEHLDEWLELPGWGFFVLGSVLIFLIGRIHELGVELADHVFHRTFQRELAS
                     LNMVAEDILQATSLDAIDATLTAAPARVLDLASSAVFRYDGAILRRHDEAIGWADGSP
                     ACLEPADPSFAPAQKGQPFPIDPDRALRFGLPVGLASPTVAVPICDRLTCHALALYGP
                     HATGADLSYDERKMLARLAEAAALAYRHVELDALRRQIAALQT"
BASE COUNT          425 a          627 c          622 g          309 t
ORIGIN      
        1 catcaggctc cagaatagcc gccaaaccgg ccgttggccg agcgaacggc actcactaca
       61 caaatggtgt cgagttgacg acttgtatcg gaaaaggctc gggccgggat cctggatccg
      121 gcggctcctt gctttgtccc gtcaccaagg atttctgtag gcgtggccta agttggtgac
      181 gacataggtc atccctgctg ccaatgaaca acttgtcttt aatcaacggg ctgtctcatg
      241 cttccagaag ccgtagccgt atagtcccat ccacggtgct gccgtcggtc gcgaccatag
      301 tggcaggtcg cacgacggtc cggtcgccta cgtctgaaga gccgcgatct ggcgccgcag
      361 cgcatccagc tcaacgtgac ggtaggcgag ggcagccgcc tccgcgagcc gcgccagcat
      421 tttgcgttcg tcgtacgaca ggtcggcgcc ggttgcgtgc gggccataaa gggcaagagc
      481 gtgacaggta aggcggtcgc agatcggcac cgcgactgtc ggtgaggcca atcccactgg
      541 taggccgaac cggagggcgc ggtcgggatc gatcgggaaa ggctggcctt tttgcgccgg
      601 agcgaaagac ggatcggccg gttccaggca tgcggggctc ccgtccgccc atccgatcgc
      661 ttcatcgtgg cgtcgaagta tggctccatc gtagcggaac accgccgacg aagcgagatc
      721 cagcacgcgg gccggggccg cagtcagtgt ggcgtcaatg gcatcaaggc tggtcgcctg
      781 caggatgtcc tccgcaacca tgttcagcga cgctaattcg cgctggaacg tgcgatggaa
      841 cacatgatca gcaagttcga ctccgagttc gtgaatacgt ccaatcagga aaatgagcac
      901 agagccgaga acgaaaaaac cccagcccgg cagctccagc cactcgtcca gatgctcgat
      961 ctgctggtgc agaaataatg ccggcacgct gagcatcagg ccgaacacgc tgatccgtcg
     1021 cagcggcatc gacacgctga ccacacgggg gcggcgcacc gcttcaaaca cgaaccaggc
     1081 gaacaggcca tgaagggcat agagcagggc gatcagatcg gaaggaaccg tggtgacgcc
     1141 gggaatgaag cgccaaagtg ttgtggactg caggatcgcg gcgacgataa aggcgggcag
     1201 gccaagcacg cagccccaga tcacccagcg caatcgctgg tattgctgcg gcgactggcc
     1261 gcggcggcgg cgcagcagta tcaggattgc agccgcatcg accacgaaac cggccaggaa
     1321 cgtcgtccgc gacaccgcct cgctcggttg cccgaaagca ttggcgtaac tcagcaattg
     1381 cagcccgatg aacagcagtg ccaccagtgg cagcacgaac cgccaaccat tccaggccgg
     1441 agacgtctga tccgacggca cgcgcagcac gaatagcagg aaaccggcgt acccaaggcc
     1501 ctgcatcacc gcgccagccg cctcttgcgc cagcaacaag tgcggccgtt cctgcaggaa
     1561 cgcatagaag acgaagttct gtccggagtt aaaccagatg gcatagagga agaagcccca
     1621 ggtcatcccg ccggggcggg tccagaccag ccaggcggcc gccaggatga acagcgccgc
     1681 gatgacctcg ttgaacagca ggacccctcg atcaagcgat gtcggttcgg tcaacttcgc
     1741 cggcacatcc acggtgcgta cggcactccc gtcagctggg aggatctgca gtcgaagcac
     1801 acgccccggc ttgacaagtt gccttccgcc catgccgccc agcaccgcga gcagatcgat
     1861 gcatcgacgg ttttgcgggt cgcggcacgt catcgcgggc aggtccagcc ggtcgcccgg
     1921 ccgcagtccc gcctgccatg ccggcgagtc ctcctgacgc gggaagggtc cctttacgtc
     1981 gta
//
LOCUS       DASWQN010001172         2396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1830797, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001172 DASWQN010000000
VERSION     DASWQN010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830797"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VGC82_19920"
     CDS             complement(<1..522)
                     /locus_tag="VGC82_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0425595.1"
                     /translation="MRFTEIGQQLRAYRLESGLRAEEIAARLGVSRAALYRYEKGEVI
                     KLDTIKRLAELLKISPLSLLGIGVEYYNRPIGYFERVRQIEETADQVLQLAGPLCYLT
                     TSDAYDSALADAFEETADQMGSERTAMRSMAEQVLGILAARKRMYAMRRPSIIAMVSL
                     PAVQRLIEVGIVSG"
     gene            complement(730..1617)
                     /locus_tag="VGC82_19925"
     CDS             complement(730..1617)
                     /locus_tag="VGC82_19925"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEY0425596.1"
                     /translation="MKIGFIGLGIMGRPMALNLKNAGHELIVPERPSLTDEIRGAARV
                     VATSKEVAAASEVVILMVPDTPDVETVLFGKDGAAEGLQKGSLVIDMSSISPIETKEY
                     AKRINAKGCDYLDAPVSGGEVGAKAASLTIMVGGPDAAFARAKPLFDLMGKNITHVGP
                     EPGAGQICKVANQIIVALNIQAVSEALVFASKAGADPAKVRQALMGGFASSRILEVHA
                     ERMLNGSFNPGFKIKLHQKDLNLALSSAKALSLSLPNTAVAQQMFASCIAHGGAELDH
                     SALVLAIEGLAAHSIRDAK"
     gene            complement(1700..>2396)
                     /gene="otnI"
                     /locus_tag="VGC82_19930"
     CDS             complement(1700..>2396)
                     /gene="otnI"
                     /locus_tag="VGC82_19930"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEY0425597.1"
                     /translation="FEGVEFLFPYEFEATAIRERLSGEGLTQVLFNMPPGDWANGERG
                     MASLPGRQPEFREAVKKALDYAARLECRQIHCMAGIVPAGVSLTTATSVYAANLAWAA
                     EQAKPAGVRVVIEPINHRDMPGYFLNTQAQGAAIIEAIGRDRLGLQFDLYHVQITEGD
                     ITKRMEQFLPVIAHMQIADVPARNEPGTGEIGWRYVFRRMDELGYTGWVGCEYRPAGD
                     TIAGLSWREEFDV"
BASE COUNT          458 a          799 c          684 g          455 t
ORIGIN      
        1 gccgctgaca atccccacct caatcagccg ctgcaccgcc ggaagcgaca ccatcgcgat
       61 aatgctcgga cgccgcatcg catacatccg cttccgcgcc gcgagaatcc ccagcacctg
      121 ctccgccatg ctgcgcatcg ccgtgcgctc gctgcccatc tggtcggcgg tttcctcgaa
      181 cgcatcggcc aacgcgctat cgtaggcgtc cgatgtcgtg agataacaga gtggtcccgc
      241 cagttgcagc acctgatccg cggtttcttc gatctgccgt acccgttcga aataaccgat
      301 cggccggttg tagtactcga ccccgatccc cagcagcgac aacggcgaga ttttcagcaa
      361 ttcggcgagc cgcttgatcg tatcgagctt gatgacttca cctttttcgt agcgatagag
      421 cgcggcgcgg gaaactccca ggcgggcagc gatctcctca gcacgcaaac cggattcgag
      481 gcgataggcc cgaagctgct gcccgatttc ggtaaaacgc atcgacataa aagcccgccc
      541 ttacgcaata gttgcgaaaa tactggaatg ccacgcacat gccggcggtt gccggagatg
      601 acccgaaccg aggtggccga acaccgaccg tctcagaacc gacgtgacag atccagttta
      661 tggcgagagc gatccaaaac tcaacatctt ttcatgtact gcgactcgtt gcacgcgtcg
      721 cgccactgat tacttggcgt cccggatact gtgagccgcc aagccttcga tcgccagcac
      781 cagggccgag tggtccagct ccgcgccgcc atgcgcgatg cacgacgcga acatctgctg
      841 cgccaccgcc gtgttcggca gcgacagact cagcgccttg gccgacgaca gcgccaggtt
      901 gaggtccttc tggtgcagct tgatcttgaa gcccggattg aacgagccat tgagcatccg
      961 ttcggcatga acctccagga tgcgcgacga ggcgaaacca cccatcagcg cctgccgtac
     1021 cttggcggga tccgcaccgg ctttggatgc gaataccagt gcctccgaca cggcctggat
     1081 attcagtgcg acgatgatct ggttggccac tttgcaaatc tggccagcac ccggttccgg
     1141 cccgacatgg gttatgttct tgcccatcag gtcgaacagc ggcttggcac gggcaaaggc
     1201 ggcatccgga ccaccgacca tgatcgtcag ggatgcggcc ttcgcaccga cctcgccgcc
     1261 ggagacgggc gcatccagat aatcgcagcc tttggcgttt atccgcttgg cgtactcctt
     1321 ggtctcgatc gggctgatcg aggacatatc gatcaccagg ctgcctttct gcaacccttc
     1381 agccgcgccg tccttaccga acagcaccgt ttccacatcg ggcgtatccg gcaccatgag
     1441 gatcacgact tccgatgcgg cggccacctc cttggaggtc gcgacgacgc gggcggcgcc
     1501 gcggatttcg tccgtcaggc tcgggcgttc gggaacgatc agctcatggc cggcgttttt
     1561 caggttcagc gccatcggac ggcccataat gcccaagccg ataaatccga tcttcatgtc
     1621 gtttgctcca ttcgcgtttg gtcagatatg ccgtcaaacg gtggttgatt gttccggcca
     1681 gcggagacag cgggcaagct cagacgtcga actcttcgcg ccagctcagc ccggcgatcg
     1741 tatcgcctgc gggacgatat tcgcagccga cccagccggt gtagccaagc tcatccatgc
     1801 ggcggaacac gtagcgccag ccgatttccc cagtgccagg ctcgttgcgc gccggcacat
     1861 cggcgatctg catgtgcgcg atcaccggca agaactgctc catacgcttg gtgatgtcgc
     1921 cctcggtgat ctgcacatga tagaggtcga attgcaggcc caaccggtcg cggccgatgg
     1981 cctcgatgat ggccgcgccc tgagcctgag tgttcaggaa atacccaggc atgtcgcgat
     2041 ggttgatcgg ctcgatgacc acgcgcacgc cggccggctt cgcctgttcg gccgcccatg
     2101 cgaggttcgc ggcataaacc gaggtcgcag tcgtcagcga tacgccggcc ggcacgatcc
     2161 cggccatgca atggatctgc cggcactcca accgggcggc gtagtccagc gccttcttca
     2221 ccgcctcgcg aaactccggc tgacgtccgg gaagactggc cataccccgt tccccattcg
     2281 cccagtcgcc gggtggcatg ttgaacagca cctgggtcag accttcgccg ctgagccgct
     2341 cgcgtatcgc cgtcgcctcg aattcatatg gaaacaggaa ctccacgccc tcgaac
//
LOCUS       DASWQN010001173         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1169819, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001173 DASWQN010000000
VERSION     DASWQN010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169819"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VGC82_19935"
     CDS             <1..211
                     /locus_tag="VGC82_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425598.1"
                     /translation="LQHVIGVVAGNRIIARPTMGVLDHYPGHATLLGQCVGDISGDRA
                     KPAVGRCGAVLASSTFETDYAPLDR"
     gene            complement(190..>1635)
                     /locus_tag="VGC82_19940"
     CDS             complement(190..>1635)
                     /locus_tag="VGC82_19940"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0425599.1"
                     /translation="LEFFAEAEAIPMSVMREGINFTWESVAEYMEALGRRRGCNVGTL
                     VGHSGIRRYVMGEAASERSEATADEIAAMQRLIRDGMYAGALGFSTSPQGRGDPAGAG
                     PDVERLALADVLGELGTGVMQVSGGAPGGTKATRQLARDLAARTGRPTIYNLVTQPID
                     KPEEWQEHLRWLEDAFKSGARCYGSCVSVVAGPIFDLRLGLDVPQDEDLTNPHSLFHG
                     MPTWDQVMALPYQERMQAFRDPAIRKSLSAEAVEGTVAQETPGQDRRGRARGFFNRRW
                     DLMRVFMTQHERNRGLEGKSVAQIAQEQGKGVMDAFLDLSLDEGLQTFFICVDRNTDP
                     AAQKQILGSPYTVIGTSDGGARPHSADRHEYSTHLLSYWVREQQVMSLEEAVHRLTGR
                     TALMHDMNDRGFVAPGKVADLVIFDPDTIASKPREPVNDMPAGGARVKRDAVGIDHVI
                     VNGNVLLDNGELTDALPGKIVRGPLYRSNGA"
BASE COUNT          285 a          547 c          500 g          303 t
ORIGIN      
        1 cttgcagcac gttatcggcg tcgtcgcagg taatcggatc attgcgcgac ccaccatggg
       61 cgtactcgat cactaccccg gtcacgccac cctactcggg cagtgcgttg gtgatatctc
      121 cggcgatcgt gccaagccgg cggtgggtcg atgcggagct gtcctggcca gctccacctt
      181 cgaaacagat tatgcgccgt tagatcgata aagcggaccc cgaacgatct tcccgggcag
      241 agcgtccgtc aattcgccat tgtccagcag cacattgcca ttcacgatca catggtcgat
      301 gccgaccgcg tctcgcttca cccgcgctcc acccgcgggc atgtcattga ccggctcgcg
      361 cggcttcgat gcgatcgtgt cgggatcgaa aatgacaaga tccgcgacct ttcccggggc
      421 cacgaaaccg cggtcgttca tgtcatgcat cagcgctgtc cggccggtga gccgatgaac
      481 cgcctcttcc agcgacatca cctgttgttc gcgaacccag taactcagta ggtgtgtgct
      541 gtattcgtgc cgatcggcgg aatgcgggcg ggcgccgcca tcgctggtcc ctatgaccgt
      601 atacggcgag ccaaggatct gtttctgcgc cgccgggtca gtgttgcggt cgacacagat
      661 gaagaacgtt tgcaacccct catctagcga cagatcgagg aaggcatcca tcacgccctt
      721 gccctgctcc tgagcgattt gcgccacgct cttgccctcc aggccgcgat tgcgctcgtg
      781 ctgggtcatg aaaacccgca tcaggtccca gcgacgattg aagaacccac gcgcccgccc
      841 gcggcggtcc tggccaggtg tctcttgggc gacggtgccc tccaccgcct cagcgctcag
      901 cgacttgcgg atcgccgggt cgcggaacgc ctgcatccgc tcctgatacg gtagggccat
      961 aacctggtcc caggtcggca tgccgtggaa caggctgtgc gggttcgtta gatcctcgtc
     1021 ctgtgggacg tcaaggccca ggcgcaggtc gaagatcggt ccggcgacga cggagacgca
     1081 ggagccatag cagcgggcac ccgacttgaa ggcgtcctcc agccagcgca gatgctcctg
     1141 ccattcctcg ggcttgtcga tcggctgggt gacgaggttg tagatggtcg gacgaccggt
     1201 ccgcgcggcg aggtcgcgag ccagctgacg tgtcgctttg gtgccacccg gggcgccgcc
     1261 ggagacctgc atgacgccgg tcccgagttc cccgagaaca tccgcaagcg ccaagcgctc
     1321 tacgtcgggc ccggcaccgg cgggatcgcc gcgtccctgt ggcgaggtgc tgaagcccag
     1381 tgcaccggca tacataccgt cccggatcag gcgctgcatc gcggcaattt cgtcagcggt
     1441 ggcttctgac cgctcggaag cagcctcgcc catcacataa cggcggatgc ccgaatgacc
     1501 gaccagcgtg cccacgttgc agccgcggcg ccggccaagc gcttccatgt attcggcgac
     1561 cgattcccag gtgaagttga tcccctcgcg catcacgctc atcgggatgg cttcggcttc
     1621 cgcaaaaaac tcgag
//
LOCUS       DASWQN010001174         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1038602, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001174 DASWQN010000000
VERSION     DASWQN010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038602"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1767
                     /locus_tag="VGC82_19945"
     CDS             <1..1767
                     /locus_tag="VGC82_19945"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0425600.1"
                     /translation="RVGAVLDRWGPQAVMPLNYAGPHGLLAYDSMSLRFFHKLGASQL
                     FRRALCGGVRSEAWVGTYGAVPGIGPDAAAQATLNVVWGNNATVTNLHLVRQVRSAQR
                     LGGRLVVIDPWRSKIAEQADLHLAIRPGTDVLLGFALAVELERLNAHDRQFISEHVLG
                     YDEYMQAARVWPAEKAAIECGVPVEQIITLATWMAEAERLVMAPGNGLERGRNGGSGV
                     RAAIALPALLGKLNGTSGIVLGSGFAFPKTVARLSRPDLIPAGTRTLNILDVGRHLDR
                     DDIDPPLRALFIYNHNPIVVHPDQNRLRRGLLREDVFTVGIEIAMTESMAFCDIVLPA
                     ATQFECDDLYGAYGHHWLQRAEPVIRPLGEALPNTEIFRRLAARFGFDDPCFKASDMA
                     LMDDALDGLDPRLNGIRPSELPTNEALRMLAPDGRPLALFDTVMPATPSGKVELVSDT
                     LASRWGERARLPGFRPGRSPRSLTLISPASDKRISSTLLGAGGTVGEAPPLLMHPDDA
                     AVRGLGSDTQVRVWNELGVVVLPLRITDAVPPGVVASEKGAWLITSPTGQTISALVSA
                     DDRADLSEGACYNDTAVEVEAV"
     gene            complement(2065..2784)
                     /locus_tag="VGC82_19950"
     CDS             complement(2065..2784)
                     /locus_tag="VGC82_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425601.1"
                     /translation="MTGEPVLDISGLCIGYGPVSAVADISLMVRNGRIATLIGSNGAG
                     KTTIMKGITGLKTPASGSVRFRGDVLTGRPVDVIVAAGISLVPEGRRLFPTMSVRENL
                     EIGAYRRTDVPAVRRDLELVLDYFPALRERLDARAMALSGGQQQMVAIGRALMAGPRL
                     LLLDEPSIGLAPAMVQTIGRIITTISQSGVDVLLVEQNAHMALSLSADAFVLETGRIV
                     MRGEAAELARSEAVRESYLGM"
     gene            complement(2781..>2990)
                     /locus_tag="VGC82_19955"
     CDS             complement(2781..>2990)
                     /locus_tag="VGC82_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425602.1"
                     /translation="AVMRRIREEFGTTILLVDHNMRAVMRICDRIVVLNHGQLLAEGT
                     PAEIRGNQAVIRAYLGDDSTMGTAA"
BASE COUNT          525 a          991 c          944 g          530 t
ORIGIN      
        1 cgcgtcggcg cggtgctgga tcgttggggt ccgcaagcgg tcatgccgct gaactacgcc
       61 gggccgcacg gcctgctggc ctacgacagc atgtcgcttc ggttcttcca caagctagga
      121 gccagccagc tgttccgccg agccttgtgc ggcggcgtgc ggagcgaggc ttgggtcggc
      181 acgtatggcg ccgtccccgg catcggtccc gacgccgcgg cacaagcaac gctgaacgtg
      241 gtctggggca acaacgccac ggtcaccaac ctgcaccttg tgcgtcaggt tcgttcggct
      301 cagcggctgg gtggccgcct ggtcgtgatc gacccgtggc gcagcaagat cgcggagcag
      361 gccgacctgc atctcgcgat tcggcctggg acggacgtgt tgctgggatt tgccctggcc
      421 gttgaactcg aacgcctgaa tgcccacgac cggcagttca tcagcgaaca tgtgctgggg
      481 tatgacgaat acatgcaggc cgcccgcgtc tggccggcgg aaaaggccgc catcgaatgc
      541 ggcgtgccgg tcgagcagat tatcacgttg gcgacgtgga tggcggaggc cgaacggctg
      601 gtcatggcgc cgggcaacgg gctggagcgt ggccgcaacg gcggcagcgg cgtgcgggcc
      661 gccattgcgt tgcccgccct gctgggcaag ctgaacggca ctagtggaat tgtgctcgga
      721 tcgggatttg ccttcccaaa aaccgtggcc cgcctgtcgc ggccagatct gataccggca
      781 ggcacccgca ccctgaatat tctcgatgtc gggcgccacc tggatcgcga cgacatcgac
      841 ccgccgctgc gagcgctgtt catctacaac cacaatccga tcgtggtgca ccccgaccaa
      901 aaccgcctcc ggcgcggtct gttgcgtgaa gatgtcttta ccgtcggaat cgagatcgcg
      961 atgaccgaaa gcatggcttt ctgcgacatc gtgctgccgg ccgcgaccca gttcgagtgc
     1021 gacgacctgt acggcgccta cggccaccac tggctacaac gagccgaacc cgttatccgg
     1081 ccgctcgggg aggcattgcc gaacaccgaa atcttccgcc ggctggctgc tcgcttcggc
     1141 ttcgacgacc cttgttttaa ggccagcgat atggcgttga tggatgacgc gctggacggg
     1201 ctggatccgc ggctgaacgg catccgcccc agcgagctgc cgaccaacga ggcgctgcgc
     1261 atgctggccc cagatggcag gccgttggcg ctgttcgata cggtgatgcc ggccacgcct
     1321 tcgggcaagg tcgaactggt gtcggatact ctcgcctcgc gatggggcga acgcgcacga
     1381 ctgccgggtt tccgcccagg ccgttcgccg cggtcgctga cgttgatctc cccagcatcg
     1441 gacaagcgga tttcatcgac cctgctgggt gcgggcggca cggttggtga agcaccgcct
     1501 ctcctgatgc atcccgatga tgccgcggtg cgtgggctcg gaagcgacac ccaggtccgt
     1561 gtttggaacg aacttggggt ggttgtgctg ccgctgcgga ttaccgatgc ggttccgccg
     1621 ggtgttgtcg cttccgagaa aggcgcctgg ctgatcacca gcccgaccgg acagacgatc
     1681 agcgcgctgg tgtcggccga cgatcgcgcg gatctgtcag agggcgcctg ctacaacgat
     1741 accgcggtcg aggtggaagc ggtgtagcga acaattacct tggacgagag gcattggctt
     1801 agccagcgac acgcgggcac gttgagggca ccctttggcg gcacttttcg ttatggcgaa
     1861 ccaagggctg cagcgttcga ttgtgtcgtg gccggcacaa ggctggccat gacactctcc
     1921 acaattgcca aagccgcgct atggcaatcg gtccgatgct catggtgaga tgccagccac
     1981 gacactgctg aaccggataa cccggctcga agcagttatt cgcgatcaaa cacctggcca
     2041 gccgtttcac atcccaaggc cgtttcacat cccaaggtag ctttcccgca cggcctcgct
     2101 gcgggccaac tccgccgcct caccccgcat gacgatgcgg ccggtttcca ggacgaacgc
     2161 gtctgccgaa agactcaacg ccatatgagc gttctgttcc accagcagca catcgacgcc
     2221 gctttggctt atcgtcgtga tgatgcggcc gatcgtctgc accatcgccg gcgccaagcc
     2281 gatcgacggt tcatccagca acagcaatcg cggccccgcc atcagcgccc ggccgatggc
     2341 caccatctgc tgctgcccgc ccgacaacgc catcgcacga gcgtccagcc gctcgcgcag
     2401 cgctgggaaa taatccagca ccagctccag gtcacgccga accgctggga cgtcggtgcg
     2461 ccggtaggca ccaatctcaa gattctcccg cacgctcatg gtcggaaaaa gccgtcgccc
     2521 ctccggaacc agcgatatgc cggcggcaac gatgacatcc accggccgtc cggtcagcac
     2581 atcgccgcgg aaccgcacgc tacccgacgc aggggttttc agcccggtga tacccttcat
     2641 aatcgtggtc ttcccggcac cgttcgatcc gatcagggtc gcgatacgac cgttgcgcac
     2701 catcaggctg atatcggcga ccgcgctgac cggcccgtag ccgatgcaga ggccggagat
     2761 gtcgagcacc ggttctcccg tcacgccgcc gtccccatcg tcgaatcgtc gcctagatag
     2821 gctcggatga cggcttggtt gccgcggatc tccgccggcg ttccctccgc cagaagctgg
     2881 ccatggttca ggaccacgat ccggtcgcag atgcgcatga cggcacgcat gttgtggtcc
     2941 acgagcagga tcgtcgtgcc gaactcctca cggatccgcc gcatgacggc
//
LOCUS       DASWQN010001175         4493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2623460, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001175 DASWQN010000000
VERSION     DASWQN010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2623460"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VGC82_19960"
     CDS             <1..720
                     /locus_tag="VGC82_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425603.1"
                     /translation="ETSTAPHPAIDLHTPLYYQLVYTLTGLLPPPLDDTQEALHTRNH
                     AAIAKVAALLPVNANEADLASQCIAARAQAEEMLRLLRQNADDISLVMRLNAQYGSMV
                     RTSLSVHGRLMRMQALRRKREVIEDAANQDAWALHIAERSMLRVVDPDAGPQQATLPR
                     GAPATLPKAAPAADVDQRIQEHVSEDGTNSQDAAFETWLSAQFQGRGPGRPDGNRLHE
                     VMREAMAKPRSAARELPANYR"
     gene            complement(830..1402)
                     /locus_tag="VGC82_19965"
     CDS             complement(830..1402)
                     /locus_tag="VGC82_19965"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0425604.1"
                     /translation="MDDADLVPTRHRLTVDDYYRMAEAGILGEDDRVELIDGELIDMA
                     SVGPDHIATVNGLTSVLVMACRDRAIVSVQNLARLDRFSEPQPDVTLFRPRADNYRTG
                     ARPRPADVLLLVEVADSSLRYDRAVKLPLYARAGIGEMWIVDLRRRVVDVHRTPEGDG
                     YATIETHGPEDTVTLALAPEIAVALRRVFA"
     gene            1660..2586
                     /gene="nudC"
                     /locus_tag="VGC82_19970"
     CDS             1660..2586
                     /gene="nudC"
                     /locus_tag="VGC82_19970"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEY0425605.1"
                     /translation="MTLILASRPNVYSSSPLDRIATRREDPAWITEKLADPDTLFVPV
                     WRTKNLVRGTSEGNPEAVYISGEMAHALRIQEGPWAFLGILDDRAVFAIDISGTDDPV
                     PLLPESLGTFVDLRSVGWGVPRPEASVLAHARGLMHWRQRHKFCGVCGNSCEVKSSGH
                     MMQCTVCNTQHFPRTDPAVIMLVHRGDRALLGHSTRFPRSTMYSTLAGFVEPGETLEE
                     AVRREVLEESAIVVGDVHYHSSQPWPFPGNIMLGFYAEALSEDITIDPEELQDARWFS
                     RQEMHDHKQHGFELPRADSIARRLIEDWLAVG"
     gene            2586..3059
                     /locus_tag="VGC82_19975"
     CDS             2586..3059
                     /locus_tag="VGC82_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY0425606.1"
                     /translation="MPNPIDLATIGYEGCTIDMVLTTLRDARVGLLIDVRAVPQSRKP
                     GFSKRQLAAALDAAGIGYVHLQGLGTPKLGRDAARAGHPERMEVIFREHMTCDRAQAE
                     LAQAKSLARERPSCLLCFERDHNMCHRRLVAEMIVAETGQAVQHLAPVVAAGTFV"
     gene            3088..4317
                     /locus_tag="VGC82_19980"
     CDS             3088..4317
                     /locus_tag="VGC82_19980"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0425607.1"
                     /translation="MDQATVAAVTWPKGLTQVPFSVYQDPELLEQEQRRLFEGPVWNY
                     LCIEAEIPNTGDWRATFVGRMPVVVVRTETGEITAFENRCLHRGALICLDDAGNARDF
                     TCVYHAWRYDLGGNLKSIAFRKGVNGKGGMPDSFRPEDLVTRKLRVTTLGGIVFGTLS
                     TITPAIEDFVGPEVCAALRLTTSRKLEVIGRFTEELPNNWKLYAENVRDTYHASLLHV
                     FFATFRINRLSQGGGVAVSENGACHVSTTLAPTEEADNAYDGIRSVDHGLKLSDPSML
                     DAEDEHGDRVRQQILSIFPSFAMQRTYNVMAIRQFLPRGVDRTDLNWIYLGYADDAPE
                     TRRRRLKQLNLAGPAGFVSMEDGCIGNFVERGSAASPSGVSLLEMGGAGTESQETRAT
                     ETAIRGFWKMWRGMMGS"
BASE COUNT          848 a         1506 c         1383 g          756 t
ORIGIN      
        1 gaaacctcca ccgcgccgca tccggccatc gatctgcaca cccccctcta ctatcagctt
       61 gtctacaccc tcaccggcct gttgccgccg ccgcttgatg acacgcaaga agcgctgcac
      121 accagaaacc atgccgccat cgccaaggtg gcggcgctgc tgccggtcaa cgcgaatgag
      181 gccgatctcg cctcccagtg catcgccgct cgggcccagg ccgaggagat gttgcggctg
      241 ctccgtcaga atgctgatga catctcgttg gtgatgcggc tgaacgcgca gtacggatcg
      301 atggtgcgga cgtcgctttc cgtgcacgga cgactgatgc ggatgcaggc gttgcgccgc
      361 aagcgggagg tgatcgagga cgcggcgaac caggacgcct gggctctgca tatcgccgaa
      421 cggtctatgc tgcgggtggt ggatcccgat gccggaccgc agcaggcgac attgccccgc
      481 ggagcgccgg caacgttgcc caaagcggcg ccggcggcgg atgtggacca gaggatccag
      541 gagcatgtct cagaagatgg aacgaattct caggacgcgg cttttgagac gtggttgagt
      601 gcgcaattcc agggtcgggg gcccggacga ccggatggga accggctgca cgaggttatg
      661 cgggaagcga tggccaaacc gcgttcggcc gctcgggaat tgcctgcgaa ctatcgctaa
      721 gcatgatcac gctgagcgga ccgttcaaag cgtgtgaatc atgcgatcaa acaaagagac
      781 agatgatttt cagcatgtcc agtcaggctg aaaacgatct cggggcccct cacgcaaaga
      841 cccggcgcaa tgccaccgcg atctccggcg ccaaagcgag cgtgaccgtg tcttccggtc
      901 catgcgtctc gatcgtcgcg tatccgtccc cttccggcgt ccggtgcaca tcgaccaccc
      961 gccggcgcag atcgacgatc cacatctcgc caattcccgc ccgcgcatac agcggcagct
     1021 tcacggcgcg gtcgtaccgc agcgagctgt ccgccacctc gaccaacaac aggacatcgg
     1081 ccggtcgggg ccgagcgccg gtccggtagt tgtcggcgcg cggccggaac agcgtgacat
     1141 ccggctgggg ctcgctgaac cggtccagcc gtgcaagatt ctggaccgaa acgatcgccc
     1201 gatccctgca agccatgacc aacacgctgg tcaggccgtt cacggtcgcg atgtggtccg
     1261 gtccgaccga cgccatgtcg atcagttctc cgtcgatcag ttccacgcgg tcgtcttcac
     1321 ccaggatgcc ggcctcggcc atccggtagt agtcgtccac agtcagtctg tgccgcgtcg
     1381 gcacgaggtc tgcatcgtcc atcgcgcacg ctctagcgat cgaggaaaac cccatgctgg
     1441 cacaccgcga accactggtc caacgacgac gaggaccaca cgggcatccg gaacggcgac
     1501 cggcactggc agggccgcgc gctttgtcag gaaacataat cctacgaacc gacccaccac
     1561 ccaattcgca tcaagccggc gtaggtcatg aaagttgcct gccacccctg acccatggca
     1621 ccgtcaccgc aagaccaccc attgccccgg aacgcccccg tgaccctgat cctggccagc
     1681 cgtcccaacg tttattcctc ctctccgctc gaccgcatcg ccacgcggcg cgaggatcca
     1741 gcctggatca cggagaaact cgccgacccc gacacgctgt tcgtcccggt ctggcggacg
     1801 aaaaacctcg tccgcggcac cagcgaaggc aatcccgaag ccgtctatat ctccggcgag
     1861 atggcccacg cgctgcgcat ccaggaagga ccctgggcct tcctcggcat cctcgacgat
     1921 cgtgcggtct tcgccatcga catcagcggc accgacgacc cggtcccgct gctgccggaa
     1981 tcactcggca ccttcgttga cctgcgctcc gtcggctggg gcgtgccgcg gcccgaggca
     2041 tcggtgctcg cccatgcacg cggcctgatg cactggcgcc agcggcataa attctgcggc
     2101 gtctgcggca attcctgcga ggtcaaaagc tccggccaca tgatgcagtg caccgtctgc
     2161 aacacccagc acttcccgcg cacggacccg gccgtcatca tgctcgtcca ccgtggcgac
     2221 cgcgcgttgc tcggtcattc cacccgcttc ccgcgctcca cgatgtattc gaccctggcc
     2281 ggtttcgtcg agccgggtga aaccctggaa gaagccgtcc gccgcgaggt gctggaggaa
     2341 tccgccatcg tcgtcggcga cgttcactac cattccagcc agccctggcc gtttcccggc
     2401 aacatcatgc tgggcttcta cgccgaagcc ctgagcgaag acatcaccat cgaccccgag
     2461 gaactgcagg acgcccgctg gttcagccgg caggaaatgc acgaccacaa gcagcacggc
     2521 ttcgaactgc ctcgcgcgga cagcatcgcc cgccgcctga tcgaggactg gcttgcggtg
     2581 ggttgatgcc caaccccatc gacctcgcca cgatcggtta tgagggctgc accatcgaca
     2641 tggtgctgac caccctgcgc gacgcccggg tcggcctgtt gatcgatgtc cgagcggtgc
     2701 cgcagtcgcg gaaaccgggt ttctccaagc gtcagctcgc ggcggcgctg gacgcggccg
     2761 ggatcggata tgtacaccta caagggctcg ggacgcccaa gctgggacgt gacgcggcgc
     2821 gtgccgggca tcccgaacgg atggaggtca tcttccgcga acacatgacg tgcgaccggg
     2881 cacaggcgga actggcgcag gccaaaagcc ttgcgcgcga acgtccgtcc tgcctgctct
     2941 gctttgaacg cgaccacaat atgtgccatc gccggctggt ggccgagatg atcgtcgcgg
     3001 agaccggaca ggccgtccag catctcgcgc cggttgtcgc cgcgggcacc ttcgtctagc
     3061 cttcagggcg cagcacggga ggatcgcatg gaccaggcaa ccgtcgccgc ggtgacatgg
     3121 ccgaagggcc tcacgcaggt gccgttctcc gtctatcaag acccggagct tctggaacag
     3181 gagcaacgga ggctgttcga aggtccggtc tggaactact tgtgcatcga ggccgagatc
     3241 ccgaacaccg gcgactggcg cgcgacgttt gtcggccgca tgcctgtcgt ggtggtgcgg
     3301 accgagaccg gggaaatcac cgcgttcgaa aaccggtgcc tgcatcgcgg cgcgctgatc
     3361 tgtctcgacg atgccggcaa tgcgcgcgat ttcacctgcg tctatcacgc ctggcgctac
     3421 gacctgggcg gcaacctgaa gtcgatcgcc ttccgcaagg gtgtcaatgg caagggcggc
     3481 atgcccgaca gcttccgtcc ggaagacctt gtaacgcgga agctgcgagt gacgacgctc
     3541 ggcggaattg tatttggcac gctttccacc atcacgcccg ctatcgagga cttcgtcgga
     3601 cctgaagtct gcgccgcgtt gcgcctgacc accagccgca agctggaagt catcggccgc
     3661 ttcacggaag aattgccgaa taactggaag ctgtatgcgg agaacgtccg cgacacctac
     3721 cacgccagcc tgttgcatgt gttcttcgcc actttccgca tcaaccgcct cagccagggc
     3781 ggtggcgtgg cggtcagcga aaatggcgcc tgccatgtgt ccacgacctt ggctccgacg
     3841 gaggaggcgg acaacgccta tgacggcatc cggtcggtgg atcatgggct gaaactgtcc
     3901 gatcccagca tgctcgacgc cgaagacgag cacggcgacc gcgtccgcca gcaaatcctg
     3961 tcgattttcc cgagcttcgc gatgcagcga acgtataacg tgatggcgat ccggcagttc
     4021 ttgccacgag gcgtggaccg taccgacctg aactggatct acctgggcta cgcggacgat
     4081 gcgcccgaga cgcgccgccg ccggctgaag cagctcaacc tcgccgggcc agccggtttc
     4141 gtgtcgatgg aggatggttg catcggcaac ttcgtcgaac gtggttcggc agcttctcca
     4201 tcgggggttt cgctgctgga aatgggcggc gccggaacgg aaagccagga gacccgagcg
     4261 acggaaacgg cgatcagggg cttctggaag atgtggcgcg ggatgatggg atcgtagcga
     4321 ctggaaaagg tgcccgccgg cttgccgtct gacgcctatg tggcccggcc acccatgatt
     4381 tcgtttgaaa ccacaacaat atcctgttcc agtcaccgtg aaccgctcta gccaggccag
     4441 ttcgtgcccc agcggtcggc gaaggcaacc tctgaaacag ggcgacgcgc caa
//
LOCUS       DASWQN010001176         4518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_247672, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001176 DASWQN010000000
VERSION     DASWQN010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247672"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VGC82_19985"
     CDS             complement(<1..277)
                     /locus_tag="VGC82_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0425608.1"
                     /translation="MTAQAFKAARDFLLAQRTDYVTAYRDFRWPEMNRFNWALDWFDR
                     ELARGDTAHQPALIIVGEGAARLTFAELSERSNRVANGLRALGVKRGD"
     gene            complement(420..1319)
                     /gene="mmsB"
                     /locus_tag="VGC82_19990"
     CDS             complement(420..1319)
                     /gene="mmsB"
                     /locus_tag="VGC82_19990"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEY0425609.1"
                     /translation="MATIGFIGLGNMGAPMAANLVKAGHAVTGYDLNPAALEALAKAG
                     GKIAKSAADAVAGAAVVITMLPAGEHVREVYLNQGGLIEATKDVKPLLIDCSTIDVES
                     ARTVTATAETAGLAMLDAPVSGGTAGAQNGTLTFMVGGTEEAFTRGKSVLEAMGKNIF
                     HAGGPGAGQAVKICNNMMLAINMVGVSEGFLLAQKLGLDWAKLHQICATATANSWALS
                     SYCPAPGPVPAAPSNRDYAPGFAAALMVKDVKLSQAAAEATGSPTPLAAKALSLYQQV
                     VDAGDGAKDFSAVFRLLAQQKRI"
     gene            complement(1322..1780)
                     /locus_tag="VGC82_19995"
     CDS             complement(1322..1780)
                     /locus_tag="VGC82_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HEY0425610.1"
                     /translation="MAEIRTSHLFTLKLAVNGMQPIGETPSGNRRVGLVAGGTFEGPK
                     LRGIVLPGGADWIIGRPDGVTTLDVRIVLQTDDGAAIGLTYKGLRHGPPEVMDKVNSG
                     QFVDPAEYYFRTTITFETAAPKYAWLNKIFGIGTGSRPPEGPVYDIFEVL"
     gene            1983..3095
                     /locus_tag="VGC82_20000"
     CDS             1983..3095
                     /locus_tag="VGC82_20000"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0425611.1"
                     /translation="MSALYPLSESNLSALGLAAKPIDHLDALIQSHIAEGRYAGAQIA
                     LARNGKLGLFRTYGNARTEDTTVPATNETLFLLFSQTKVLTSSAVWTLVEEGKLSFMD
                     KVADHLPEFAARGKGDITLQQVMTHQGGFPNADVTEATWTDHALMRAQVCDFSLEWTP
                     GTRLHYHGRAAHLVQAMVIEAVTGQDFRDVIRERVIEPLGLGNDIFVGVPEAQQNRCA
                     DTYGPEKRDNSAAFRSAGLPSGGGYATARGMAAFYQMLGHGGRLNNVRLFSPRLIAYV
                     SRGHTGESPDQAMTGIPMHRGLGPHVRGDSDRIRGLGTIGAPVTFGHGGVGSSYSWTD
                     PSSGVSFSYLTNFVSPDPWHSARLDRVANLVHAAID"
     gene            complement(3420..4331)
                     /locus_tag="VGC82_20005"
     CDS             complement(3420..4331)
                     /locus_tag="VGC82_20005"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0425612.1"
                     /translation="MAPLPTKPTSFGASYLLDQYPAILVLHILALHGTGRTPTMAQQP
                     IRFDDGEAYERGMGIWSRLAGQVFLDWLACPPGLRWIDVGCGSGAVTELLVQRCAPAE
                     TQGIDPSPAQLAFARMRPGAQGATFLPGDAMALPFDADRFDAAVMALVIFFVPDPAKG
                     VAEMVRVVRPGGTVAAYAWDILGGGFPFDPIQAELRDMGLTPPLPPSVGASRIAAMRD
                     LWAGAGLEAVETREISVRRSFANFEEFWSTSTTSGSIRPTLAGMAPGDVEKLQAQVRA
                     RLSADATGPVTWRARANAIKGRVPKAP"
BASE COUNT          772 a         1478 c         1456 g          812 t
ORIGIN      
        1 ggtcgccccg tttgacaccg agggcgcgga gaccattggc aacgcggttg gaccgttcgg
       61 agagttccgc gaaggtcagc cgggcggcgc cctcccccac gatgatcagg gcgggttggt
      121 gggcggtgtc gcctcgggcg agctcgcggt cgaaccagtc gagcgcccag ttgaaccggt
      181 tcatctcggg ccagcggaaa tcgcgataag cagtcacgta gtcggtacgt tgggccagca
      241 gaaaatcgcg ggcagctttg aatgcctggg ctgtcatggg tgtttgccct tgatgatgcg
      301 agcgatgata ggctggatcc cccaatccgt cacgatgaac gatgtccgga aactacccgc
      361 ctcgtcgcct gaagccgcgg atgaaggcgc ttcggccggc atgaccagaa gtgccgccct
      421 caaatccgct tttgttgcgc gagcaggcgg aacaccgcgg aaaaatcctt tgcgccgtcg
      481 ccggcatcga ccacctgctg gtacagactt agtgctttag ccgccagcgg tgtgggcgat
      541 ccggtcgctt ctgccgccgc ctgggacagt ttcacgtcct tgaccatcag cgccgcggca
      601 aaaccaggcg catagtctcg attcgagggt gccgcgggca ccggtccagg cgccgggcag
      661 tagctcgaca aggcccatga gttggcggtc gcggtggcgc agatctgatg cagcttggcc
      721 cagtccagac cgagtttctg ggcgaggagg aagccttccg agacgccgac catgttgatc
      781 gcgagcatca tgttgttgca gatttttact gcctgtccgg cgcccggacc gccggcgtgg
      841 aagatgttct tgcccatcgc ttcaagcacg gatttgccgc gcgtgaaggc ttcctccgtc
      901 ccgcccacca tgaaggtcag ggtgccgttc tgcgcgcccg ccgtgccgcc ggagaccggg
      961 gcgtccagca ttgccagccc tgccgtctcg gcggtggcag tgacggtgcg ggcgctttcg
     1021 acgtcgatgg tagagcagtc gatcagcagc ggtttgacgt ctttcgtcgc ctcgatcaga
     1081 ccgccttggt tgaggtaaac ttccctgacg tgctcgccgg ccggcagcat ggtgatgacg
     1141 accgcggcgc ccgcgactgc gtcagccgcg cttttagcga ttttgccgcc ggccttggcc
     1201 agggcttcaa gtgccgccgg attaaggtcg tagccggtga cggcgtggcc ggccttcacc
     1261 aggttggccg ccatcggggc gcccatgttg cccaggccga taaatccgat tgtcgccatg
     1321 atcagagtac ctcgaagatg tcataaaccg gtccctcggg cggacggctg cctgtcccga
     1381 tgccgaaaat cttgttgagc caagcgtatt tcggagcagc ggtttcgaag gtaatggtcg
     1441 tgcggaagta gtattccgcc ggatcgacga attggccgct gttgactttg tccatcacct
     1501 cggggggacc gtggcgaagg cctttgtagg tcaggccgat cgccgcgcca tcatcggtct
     1561 gcagcacgat acggacatcc agcgtggtga cgccgtcggg gcggccgatg atccagtcgg
     1621 cgcccccggg caggacgatg ccgcgcagtt tcgggccctc gaacgtgccg ccggccacca
     1681 ggccgacgcg gcggttgccg gagggagttt cgccgatcgg ctgcatgccg ttgaccgcga
     1741 gcttcagcgt gaacaggtgc gacgtgcgga tttcggccat ctggcgtcct cggtgcgggt
     1801 gggcggatca cacccgatcc gggatagaag gacaagggag gcggtggcag gtggtgggcg
     1861 gacgtgcggc ttggacaatg gcgaactaat ccgtctcatg gggtccgtcg tctcgtggcc
     1921 gattggcgcc cggtgcaatc tgggcgtaac ctccggaaaa cccatccgca ggaggaaccg
     1981 ccatgtccgc cctctacccg ctttccgagt ccaacctttc ggcgctcggt cttgccgcga
     2041 aaccgatcga ccatctcgac gcgttgatcc agtcgcacat tgccgaaggt cgctacgccg
     2101 gcgcccagat cgccctcgcc cgcaacggca agctgggcct gttccgcacc tacggcaacg
     2161 cccgtaccga agacacgacc gttccagcaa ccaacgagac gctgttcctg ttgttcagcc
     2221 agaccaaggt gctgacctcc tccgcggtct ggacgctggt cgaggaaggc aagctgtcct
     2281 tcatggacaa ggtcgcggac cacctgccgg agttcgccgc acgtggcaaa ggcgacatca
     2341 cgctgcagca ggtcatgacc caccagggcg gctttccaaa tgccgacgtg accgaggcga
     2401 catggaccga ccacgccctg atgcgtgctc aggtttgcga cttctcgctg gaatggaccc
     2461 ccggcacccg cctgcactac catggccgag cagcgcatct ggtgcaggcg atggtgatcg
     2521 aagcggtcac cggccaggat ttccgcgacg tcatccgtga acgggtcatc gagccgctgg
     2581 gccttggcaa cgacatcttc gtcggcgtgc cggaagcgca gcaaaaccgc tgcgccgaca
     2641 cctatgggcc ggagaaacgc gacaactccg ccgccttccg ctccgctggc ctgcccagcg
     2701 gcggtggata tgccaccgcc cgaggcatgg ccgcgttcta ccagatgctc ggtcacggcg
     2761 gccggctgaa taacgtgcgg ctattctcgc cccggctgat cgcctacgtc tcgcgcgggc
     2821 ataccggcga gagccctgac caggcgatga ccggcatccc gatgcatcgc ggtctcggtc
     2881 cccatgtccg cggcgacagc gaccgcatcc gcggcctggg tacgatcggc gcgccggtga
     2941 cgttcggaca cggcggcgtt ggctcatcgt attcctggac ggacccgagc agcggggttt
     3001 cgttctcata cctgacgaat ttcgttagcc ccgatccttg gcattcggcg cggctggatc
     3061 gcgtggcgaa cctggtgcac gcggcgatcg actagacgct ttaatgtcac gtcggcaggg
     3121 ataacaacca cggcggcggg cggccgatga cgccgttgcc gccgtggttg cgaagtataa
     3181 cgcttcacaa gggagggacc cggagaaact caaccaagtg acggtcaccc tgcccgacct
     3241 gggtgccggc tggcgctgct atcgcggccc ggcctggttc ccgtcgttga cgccaggcgc
     3301 cggtattggg ccggcgagaa ccgcctcagt ccgccgtgcc gggttgacgc ctcgcttcgt
     3361 tacgcacgac ggatcagtcc cggcgatacc acgtgtaaac cctgccgccg ctttacggat
     3421 catggcgcct ttggcacgcg tccctttatc gcgttggcac gcgcacgcca ggtaaccggc
     3481 ccagtggcgt ccgccgacaa gcgcgcgcga acctgtgcct ggagtttttc tacatcgccg
     3541 ggagccatcc cggccagggt cggccgtatg ctccccgagg tcgtgcttgt gctccaaaat
     3601 tcctcgaagt tggcaaaact ccgccgcacc gagatctccc gcgtctcgac cgcctccaac
     3661 cccgccccgg cccataggtc ccgcatcgcc gcgatgcgtg atgcgccgac gcttgggggc
     3721 aatggtggcg tcaaacccat gtcgcgtagt tcggcctgga ttgggtcgaa cgggaagccc
     3781 ccgcccaaga tgtcccaggc atatgccgcg accgtgccgc ctggccgaac cacccgcacc
     3841 atctcggcga cgcctttcgc cggatcggga acaaagaaga tcaccaacgc catgaccgcc
     3901 gcatcgaagc ggtcggcgtc gaacggcaat gccatggcgt cgcccgggag aaatgtcgca
     3961 ccctgtgccc ccggccgcat acgcgcgaag gctagttgcg ccggggacgg atcgatccct
     4021 tgggtttccg caggagcaca tcgctggacc agcaactcgg ttacggcgcc actgccgcag
     4081 ccgacatcga tccaccgcag gcccggggga cacgccagcc aatcgagaaa gacctgcccc
     4141 gcgagccggc tccagatacc catcccgcgc tcgtaggctt ctccgtcatc gaagcgaatg
     4201 ggttgttgcg ccatcgttgg cgtcctccct gtcccgtgca atgccagtat gtgcaatacc
     4261 agtattgccg gatactggtc caagagatag ctggccccga agctggtcgg cttggtcggg
     4321 agaggcgcca ttggcgggac tcccgatgtc gtcgtggcgg caggagaccg ccaggcgcaa
     4381 tggacttagg ggtgcgtttc gtaaaccgtc acgccgtccg ggaaaatgat ccacggctgt
     4441 gcgctacgcg tccagtagtg ggtcgtcggc ctcagccagg atgtgtcatc caaggttccc
     4501 gcccggacaa cccgcgtt
//
LOCUS       DASWQN010001177         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_907594, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001177 DASWQN010000000
VERSION     DASWQN010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907594"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VGC82_20010"
     CDS             complement(<1..260)
                     /locus_tag="VGC82_20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEY0425613.1"
                     /translation="MPRILLAAAFSAGIALSGCGDEATLPSSAGIGPNPELPPPHETL
                     ITTVNIAPAKGWPAGTSPTAAPTLKVAAFAAGLDHPRWLFVLP"
     gene            complement(346..969)
                     /locus_tag="VGC82_20015"
     CDS             complement(346..969)
                     /locus_tag="VGC82_20015"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY0425614.1"
                     /translation="MIEPRVITVFTDYKSPYAYLAKDLAYELERDCSVRLNWQPYILD
                     IPSFLGSASVDGAGRVLEESRNAHQWRRVRYSYMDCRRQARKRGLTIRGTQKIWDSSL
                     AAAGMLFARQAGDIVFRRYHDMVFERFWSRALDIENIGAVAEVLTEAGADASEFSARS
                     EALREQVAAISRAAESQGVFGVPTFVFGGEIYWGREHLPDIRQLLAD"
     gene            complement(1021..2067)
                     /locus_tag="VGC82_20020"
     CDS             complement(1021..2067)
                     /locus_tag="VGC82_20020"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0425615.1"
                     /translation="MSQIITRRQAVSGLATLAAAPLPAFAAEAPDIKALEAAAHSEAG
                     LTWYAAQVDAETAERMGRTFSQTYPGVNVGVIRTTGQVAYQRLLLDIKNTTPQCDVFS
                     ATDISHMPALKERHELSEYVPRNAEALLPPFKALSDPGFSYITNAVRYFLIYNKDKVK
                     PEDAPRAWTDLLDPKWKGRVATGHPAFSGCTGTWAVGVKKVHGWEFFEKLAANNPRIG
                     RSAVDPTTLINAGECLVGVGAAANVYASADKGNPLGMMHPTDGLVLCVTPSAIPARAP
                     HPNAARLFMEWLLSPAYARMLTTDGSEPILEGIAPRPGMPPLAEQKIIPLTVDEIRIG
                     VPLVIEQWRDTFGS"
BASE COUNT          371 a          653 c          730 g          411 t
ORIGIN      
        1 ggcagcacga aaagccaacg cggatggtcg aggccggcgg caaaggcggc taccttcaat
       61 gtcggcgccg cggttgggga cgtgccggcg ggccaacctt tcgccggggc gatgttgacg
      121 gtggtgatga gggtttcatg cggtggcgga agttccggat tgggaccgat acctgcggac
      181 gacggcagcg tcgcttcgtc cccgcaaccc gacaaagcga ttccggcaga gaaagcagcg
      241 gccaaaagga tacggggcat aaggttggtt tccgcgtctg gcgattgagc gatgagcgtg
      301 gctgtacttg tggcggcctt tgtgcattga aaccatggtg cacggtcaat cggccagaag
      361 ctgccttatg tcgggcaggt gctcgcgccc ccaatagatc tcgccaccga aaacgaacgt
      421 gggcacgccg aacacgccct ggctttcggc cgcacggctg atcgcggcga cctgctcccg
      481 cagcgcttcg gaccgggcgg aaaattcgga cgcgtccgcc ccggcctcgg ttaaaacttc
      541 agcgacggca ccgatgtttt cgatgtccag tgcgcgcgac cagaagcgct cgaacaccat
      601 gtcgtggtag cggcggaaca caatgtcgcc ggcctgccgg gcgaacagca tgccggcggc
      661 tgcgagggat gaatcccaga tcttctgggt gccccggatc gtcaggcctc gtttgcgagc
      721 ctggcggcgg cagtccatgt agctgtagcg cacccggcgc cactggtggg cgttgcggga
      781 ctcctcgagg acacgaccag ctccatccac gctggccgac cctaggaagc ttggtatgtc
      841 gagaatatag ggctgccagt tcaggcggac ggaacagtct cgctccagtt cataggcgag
      901 gtccttcgcc aggtaggcgt aagggctttt gtagtccgtg aagacggtga tcacgcgggg
      961 ttcgatcatt ggcttctggt cccatcgtct tgcgccgcgg tcattactcc gccgtcgcca
     1021 tcaactgccg aacgtgtccc gccattgctc gatgaccagc ggcacgccga tccggatttc
     1081 atcaaccgtc agcggaatga ttttctgctc ggccaagggc ggcatgcccg gacgcggcgc
     1141 tatgccttcc aggatcggct cgctgccgtc ggttgtcagc atccgggcat aggccgggct
     1201 gagcagccat tccatgaaca accgggcggc gttggggtgc ggggcgcggg ctggaatggc
     1261 cgatggcgtg acgcacagca ccaggccatc ggtcggatgc atcattccca gcgggttgcc
     1321 cttatccgcc gaggcgtaaa cattggcggc ggcgccgaca cccaccaggc actcacctgc
     1381 attgatcagc gtcgtcggat cgaccgccga acgaccaatg cgcggattgt tcgcagcgag
     1441 cttttcgaag aattcccagc catggacctt cttcacgccc acggcccagg ttccggtgca
     1501 gccgctgaag gccggatggc cggtggccac cctgcccttc cacttgggat cgagtaggtc
     1561 ggtccaggcc cggggagcat cctcgggctt caccttgtcc ttgttgtaga tcaggaaata
     1621 ccgcacggca ttggtgatgt aggagaagcc tggatccgat agcgccttga acggcggcag
     1681 cagcgcctcg gcgttgcgcg gcacgtactc gctcagttcg tgacgctcct tcagcgccgg
     1741 catgtgggag atgtcggtgg cgctgaagac gtcgcattgc ggcgtcgtgt tcttgatgtc
     1801 cagaagcagg cgttgatagg ccacctggcc ggttgtacgg atcacgccga cattgacccc
     1861 gggataggtc tggctgaagg tccggcccat tctttccgcc gtttcggcat cgacctgggc
     1921 ggcgtaccat gtgaggccgg cttcggaatg tgccgcggct tccagtgcct tgatgtccgg
     1981 cgcctcggca gcgaacgcgg gaagcggggc ggcggccaag gtagccaggc cggacacggc
     2041 ctgccggcgg gtgatgattt gcgacattgt tcctcctggt cggagttacg catggtggcc
     2101 gcgatcgctc aaccgccgaa tgctttggcg cgggattttg gccgggggac gattggcggc
     2161 cgtga
//
LOCUS       DASWQN010001178         2649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1698845, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001178 DASWQN010000000
VERSION     DASWQN010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698845"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..920
                     /locus_tag="VGC82_20025"
     CDS             51..920
                     /locus_tag="VGC82_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0425616.1"
                     /translation="MEAVTRIERTLSASLDRVDSSGGPPLLAAAMRHAVFPRGARIRP
                     RLCLAVAAACGEDDPTAAEGAAAAIEFMHCASLIHDDLPCFDDADTRRGRPSVHRAFN
                     EPLAVLAGDALIVLSFQTIAWNAFRFPARLPALILALANATGMPSGIAAGQAWECEPA
                     IDLGQYQRAKTGALFAAATILGAAASGAEAEPWRELGERLGEAYQVADDIADLLSGEA
                     ELGKPAGQDLALGRPNAALSLGIGGAMRHLEQLAAAAADSIPSCPGASSLRALTLSEA
                     RRLLPKDISQRAA"
     gene            935..2092
                     /locus_tag="VGC82_20030"
     CDS             935..2092
                     /locus_tag="VGC82_20030"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0425617.1"
                     /translation="MAIGDWWFGLRNRLLADPGFQRWAVAFAVTRPIARRRAAALFDL
                     CAGFVYSQILLACVRLHVFDALREGPRSLADLADLLSLPPADASRLLEAATALRLLDR
                     RRPDHYGLGTLGAALVGNPGVTAMIEHHTLLYADLRDPVALLRRQQRDTALGQLWPYA
                     AAAKPADLPADDVAAYSNLMAASQPLVAADVLKAYRLERHRCLLDLGGGDGTFLIQAA
                     AATPGLNLILFDVPAVAERARTRLAEAGLASRAEIFGGDFRTDQLPDGADIVSLIRVI
                     HDHDDDAALAILSAAWHALPPGGTLLLAEPMAGTRDAGGVEAYFAFYLLAMGSGRPRR
                     GTCRARPHRRVHQPAPVADASPDAGSGTHGDKVMRAARCKTNLTCQNVNLG"
     gene            2104..>2649
                     /locus_tag="VGC82_20035"
     CDS             2104..>2649
                     /locus_tag="VGC82_20035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY0425618.1"
                     /translation="MPEVQVDRAKGAGMEALAVVLNGPECLSLERLVLTSPGELDVVV
                     DVAFSGISTGTERLLWQGRMPPFPGMGYPLVPGYESVGRIVAAGAGNETRIGQTVFVP
                     GASCFGEVRGLFGGAASRLVVPASRAMPIDSSLAERGILIALAATAHHAIAGGELPGL
                     IVGHGVVGRLLARLVVVAGGQP"
BASE COUNT          411 a          877 c          858 g          503 t
ORIGIN      
        1 atgacactcc caattgacaa ccaaaataga cagttcgtcg gaggaccgcc atggaggccg
       61 ttacccggat tgagcggacc ctgtcagcct cgctagaccg agtcgatagc agtggcgggc
      121 ctccgttgct cgctgccgcc atgcgacacg cggtctttcc tcgtggcgcc cgtatccgcc
      181 cccggctttg cctcgccgtg gccgccgcct gcggtgaaga cgatccgacg gccgccgagg
      241 gcgctgccgc ggcgatcgag tttatgcact gcgcgtcgtt aatccacgac gatctgcctt
      301 gttttgatga cgccgatacg cgccgaggcc gtccctccgt ccatcgcgcc ttcaatgagc
      361 cgcttgccgt tctggcgggc gatgcgctga tcgtgctgtc cttccagacc atcgcctgga
      421 atgccttccg cttccccgct cggttgccgg cgctgatcct ggcactggcg aacgcaaccg
      481 ggatgccgtc cggcatcgcc gccggccagg cctgggaatg cgagccggcg atcgaccttg
      541 ggcaatacca gcgtgccaaa accggcgcct tgttcgccgc cgccaccatc ctgggcgccg
      601 ccgcatcggg tgccgaagcg gaaccctggc gtgaactcgg ggaacggcta ggggaggcct
      661 accaggtagc cgacgacatc gccgacctat tgtccggaga ggctgaactt ggcaaaccag
      721 caggccagga cctcgcgctg ggacgcccga acgccgcatt gtccttggga ataggcggtg
      781 cgatgcgcca cctggaacag ttggcagccg ctgcggccga ctcgataccg tcttgtccag
      841 gcgcgtcgag cctgagggcg ctgaccctga gcgaggcacg ccgtctgctt ccgaaggaca
      901 tctcgcaacg cgccgcgtga tgccgaggag catattggca atcggcgact ggtggttcgg
      961 gctgcgaaac cggctgcttg ccgatccagg attccagcgt tgggccgttg cgttcgcggt
     1021 gacccgaccg atcgctcggc gccgggccgc agcgcttttc gacctctgtg cgggctttgt
     1081 ttacagccag atcttgctgg cctgtgtccg gttgcatgtt ttcgatgctt tgcgcgaggg
     1141 accacggagc ctcgcagacc tggccgacct gttgtcactg ccgcccgccg atgcgtcccg
     1201 tctgttggaa gcggcaacgg cgctgcgtct gctggaccgc cgccgccccg atcattacgg
     1261 tcttggcacc ctgggggcgg ccttggtcgg aaaccccggt gtaacggcga tgatcgagca
     1321 tcacactctg ctttatgccg atctgcgaga cccggttgcg ttgctgcgac ggcaacagcg
     1381 cgacactgcg ctggggcagt tgtggcccta cgccgcagcg gcaaagccag cagacttacc
     1441 cgcagacgac gtcgcggcct acagcaattt gatggcggca tcgcagccgc tggtcgccgc
     1501 cgatgtgctg aaggcctacc ggctggaacg ccatcgttgc ctgctggatc tgggtggagg
     1561 cgatggaacc ttcctgatcc aggcagccgc cgccactccc gggctgaacc tgattctgtt
     1621 cgatgtaccc gctgtcgccg aacgcgcccg cacgcgcctc gccgaggcag gattggcgtc
     1681 gcgcgccgag attttcggcg gcgattttcg caccgatcaa ctgcccgatg gggccgacat
     1741 cgtctctctc atcagagtga ttcacgatca cgatgacgat gccgccttgg cgatcctgag
     1801 tgccgcctgg cacgcccttc cgcccggcgg aacgctgcta ctggccgaac ccatggccgg
     1861 tacgcgcgat gccggtgggg tcgaggccta tttcgccttc taccttttgg cgatgggcag
     1921 cggccggccg cgccgaggaa cttgccgcgc tcgtccacac cgcagggttc accaacctgc
     1981 gcctgttgcc gacgcgtcgc ccgatgctgg ttcgggtact cacggcgaca aagtaatgcg
     2041 cgctgccaga tgtaaaacga atttgacatg tcagaatgtc aatctaggat gacagcaagg
     2101 ctcatgcctg aggtccaagt ggaccgggcg aaaggagcag ggatggaagc tttggcggtg
     2161 gtgctgaacg gacctgaatg tctctcgttg gagcggctcg tcctgacgtc gcccggcgaa
     2221 ctggatgtgg tcgttgacgt cgcgttcagc ggcatcagca ccggcaccga gcgcctgttg
     2281 tggcaaggcc gcatgccgcc atttccgggg atggggtatc cgctggtccc cggctatgaa
     2341 tccgttggcc gcatcgttgc tgccggtgcg ggcaacgaga cgcgcatcgg ccagacagtc
     2401 ttcgttcccg gcgccagttg cttcggcgaa gtacgcggcc tgttcggcgg cgcggcatca
     2461 cgcctcgtcg tgccagcctc ccgcgccatg ccgatcgatt cgagcttggc cgaacgcggc
     2521 atcctgattg ctcttgccgc cacagcccat catgcgattg ccggcggcga actgcctggc
     2581 ctgatcgtcg gccacggcgt ggtcggcagg ctgttggccc gtctggttgt cgtggccggc
     2641 ggccaaccc
//
LOCUS       DASWQN010001179         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1435896, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001179 DASWQN010000000
VERSION     DASWQN010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435896"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..649)
                     /locus_tag="VGC82_20040"
     CDS             complement(293..649)
                     /locus_tag="VGC82_20040"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEY0425619.1"
                     /translation="MARKPDRSSSASPLAIDPEREQRVRERAYAMWENDGKPHGRDVE
                     YWERAREMIGKEDSASSEVSENSRKNAETPTQARSEEPSIQQNLRQGPGRAAERGEVQ
                     PAAPAKRRARAKPKKP"
     gene            complement(876..1691)
                     /locus_tag="VGC82_20045"
     CDS             complement(876..1691)
                     /locus_tag="VGC82_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY0425620.1"
                     /translation="MKISDFDALTFDCYGTLIDWDSGIFAALAPLLSRAGIGAAGSGS
                     ALSRAGMGLSRSGMELSRDAVLEAFGRLEARQQEKTPDMIYSDLLAEVHGQLAIEWDV
                     ARNPAEDADFGRSIGAWPAFPDTVEALRYLRQHFRLVILSNVDRDSFRATNQRLGVTF
                     DAVYTAQDIGSYKPDPRNFSYLVDRLSERGVPKEKILHVAQSLFHDHGPAKAMGLSSA
                     WIDRRGDADRGAMPEVRYDVRFTSLEALAEAHRAESAVALASAPPDHSSRGAA"
BASE COUNT          354 a          673 c          592 g          383 t
ORIGIN      
        1 agctgcacac gagggttcga ttcccttcac ccgctccaac tccttcaggt tttcggcacg
       61 atgctgaaag agggttcgga ttggatccta catcatcccg gccttcaccc tgcgaaattg
      121 ctgacaaata actgcttgga gctgagtgat tcggctcagc gatgtcgtgg tcggcatccc
      181 agccatgagc gtcgtactag gcgtttgcag cctgaccagc aattctagct gcgcttgatt
      241 ggatgggttt ccaacacatc ccaacgggca agctgtcgga atctggcgtt tcctatggct
      301 tcttgggctt tgcgcgggcg cgccgtttcg ctggcgcggc cggctggacc tcaccccgtt
      361 cggcagcacg acctggacct tgacggaggt tctgctggat actcggttcc tcgctccggg
      421 cctgcgtcgg cgtttcagcg tttttccggg agttttccga cacctcgctg ctggcgctgt
      481 cctccttgcc gatcatttcc cgcgcccgct cccagtactc cacgtcccgg ccatgcggct
      541 tgccatcgtt ttcccacatc gcatacgcgc gttctcgaac gcgctgttcg cgctcggggt
      601 cgatggccag cgggctagca gacgatgaac gatccggttt tcgtgccatg gggcctctcg
      661 tgggtaatca ggcgggatag cgaacggatg acagcagtgt cgcacggccg tcgcggcgtc
      721 cggatgcgac accgtcacga gacgcctgcg aagcaaatgc cccggcctaa gccgtcggtc
      781 aagaaaagcc gtagtgcagc gcgtcgtccg tcggttggcg ctgtcagaca cgcagcgact
      841 cccggacccg cacagcccct ggccaggatg ctggatcagg cagcgccgcg actggaatgg
      901 tcgggcggcg ccgaggccag ggcgacagcg gattccgctc gatgtgcctc cgccagcgct
      961 tccaggctgg tgaaccggac atcgtatcga acttccggca tcgcaccccg gtccgcgtcc
     1021 cctcgccggt cgatccaggc cgaactcaac cccatcgcct tggccggacc gtgatcgtgg
     1081 aacaggcttt gtgctacgtg caggatcttt tccttcggca ctccccgctc gctgagccga
     1141 tcaaccaggt agctgaaatt ccgcgggtcc ggcttgtagg agccgatatc ctgcgcagtg
     1201 tacacggcgt cgaacgtgac ccccagacgc tggttcgtcg ccctgaaact gtcccggtcc
     1261 acattggaca ggatcaccag ccgaaagtgc tggcgcaggt accgcaacgc ctcgacggtg
     1321 tccggaaacg ccggccacgc cccaatcgat cgcccgaaat cggcgtcctc ggccggattt
     1381 cgagcaacat cccactcgat ggcaagctgt ccgtgcacct cggccagcag gtccgaatag
     1441 atcatgtccg gcgttttctc ttgctgccgc gcttccaagc ggccgaaggc ttccagcacg
     1501 gcatcgcggg acagttccat gcctgaccgc gacagcccca tgcctgctct cgacaacgcg
     1561 ctgccgctgc ccgccgcgcc gatccccgct cgcgacagca aaggcgccag ggcggcaaag
     1621 atcccgctgt cccaatcgat cagggttcca tagcagtcga aggtcaaggc atcgaagtcc
     1681 gatatcttca tggcatggcc tccacgcgcc gcgtcaaaaa cggcgaccgg tcatggccgc
     1741 agtcggccgt gtcacaagga acaacatcgc tgctaatcgc gcgcggctca acatttggcc
     1801 tttccgctcc gatcaccaac ctgtaatgcc ggccaacgga aacggccggg aggcaatcta
     1861 aacgccatag gcggagtcgt cgttcggcac gacgccggga ccatggttcc tgacgcggcg
     1921 gcgatgtgcc ggttcggaag ctcatggtcg taccacgccc ccagccagcc gcaaggcggt
     1981 gttacgaaag ccaagcgggt tc
//
LOCUS       DASWQN010001180         4238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1831608, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001180 DASWQN010000000
VERSION     DASWQN010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831608"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /gene="ffh"
                     /locus_tag="VGC82_20050"
     CDS             <1..1365
                     /gene="ffh"
                     /locus_tag="VGC82_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY0425621.1"
                     /translation="DKLSGVFDRLRTRGSLNEADVTEALREVRLALLDADVALPVVRD
                     FIAKVRERAIGAEVLSSVTPGQQVVKIVNDVMIEALGGAGAVPINLNAAAPVPILMVG
                     LQGSGKTTTSGKIARRLKDRLKRRVLLASLDTQRPAAQLQLAQLAEQAGVPSLPIVAG
                     QTPVQIATRAMDVGRREGFDVVILDTAGRLSIDQELMDEVRQVRAVTNPAETLLVVDA
                     MTGQDAVNTAKAFNDALSITGIVLTRMDGDARGGAALSMRAITGAPIKLIGVGEKQDA
                     LEDFHPERVAGRILGMGDIVGLVERATDTIDQAEAEKLAAKMMKGKFDLEDYASQLRQ
                     ISKMGSLSGILGMLPGAGKIKQQLEGADLDTKVLKRQAAIISSMTIKERRTPDIIKAN
                     RKKRIAAGSGTTVQDVNRLLKQFDDMSTMMKKMSKLGQKGLMRHGLGALMPKGAPQQH
                     RRPF"
     gene            1402..1749
                     /gene="rpsP"
                     /locus_tag="VGC82_20055"
     CDS             1402..1749
                     /gene="rpsP"
                     /locus_tag="VGC82_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY0425622.1"
                     /translation="MGLKIRLARAGAKKRPYYHIVIADSRSPRDGRFIERVGSYNPML
                     PAEHADRVRLQPERLAHWIGNGAVATDRVAKFLGKAGLAPMPVWTEQPQQSAPKKKAQ
                     ERAKAVAAASAAA"
     gene            1809..2357
                     /gene="rimM"
                     /locus_tag="VGC82_20060"
     CDS             1809..2357
                     /gene="rimM"
                     /locus_tag="VGC82_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013220.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY0425623.1"
                     /translation="MPPGRIQLGVVGRAHGVRGLVRITSHTAAPADLTAYGPLSDPEG
                     RLYIVSWKGEGIAEIARMIDGVAVKVTDRAQAEKLTNTKLFVDRAALPPAGEDEFYLT
                     DLIGMAAIHSDGAPIGTVSAVHDYGAGASLEITGSDGPVIVPFTAACVPMVDIAAGRL
                     VVTMPDEIEVHPDTSRDAEEAA"
     gene            2354..3040
                     /gene="trmD"
                     /locus_tag="VGC82_20065"
     CDS             2354..3040
                     /gene="trmD"
                     /locus_tag="VGC82_20065"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY0425624.1"
                     /translation="MTWRASVLTLFPAMFPGPLGQSLAGRALENAVWSLSVTNIRNFA
                     QDRHRTVDDTPFGGGAGMVMRPDIVDAAVQSVADDRPVVYLTPRGRRFTQAQARHFAG
                     GPGVILLCGRYEGIGQRVVEARGMQELSIGDYVLSGGELAALVLLDSVVRLLPGVMGA
                     AESAAEESFSGGLLEYPHYTRPAEWQGRRVPDVLLSGHHGAVSVWRQAESERITRERR
                     PDLWPSAAIP"
     gene            3062..3463
                     /gene="rplS"
                     /locus_tag="VGC82_20070"
     CDS             3062..3463
                     /gene="rplS"
                     /locus_tag="VGC82_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY0425625.1"
                     /translation="MNIIQQFEAEQVARLTATRAVPAFIPGDTLRVSVKVVEGERSRV
                     QAFEGVCIARSNKGLNSNFTVRKISYGEGVERVFPLYAPTIAEIAVVRRGDVRRAKLY
                     YLRGRSGKSARIAERARSATPVAPTEPAAAE"
     gene            complement(3538..3798)
                     /locus_tag="VGC82_20075"
     CDS             complement(3538..3798)
                     /locus_tag="VGC82_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425626.1"
                     /translation="MMGIPIPAALLAIDPLTAVGVLLATAATDAVYVMFSSAIAARRR
                     LPAATWSSVWYLLSSFAVISYTQNWAYVAFAGSGHGWAPLPP"
     gene            complement(3795..4235)
                     /locus_tag="VGC82_20080"
     CDS             complement(3795..4235)
                     /locus_tag="VGC82_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY0425627.1"
                     /translation="MCRSKGIMLAIAESCTGGLIAAALTALAGSSDVLDRGFVPYSNE
                     AKNQMIGVPMELIECHGAVSEQVAVAMADGALARSRAAIAVSVAGVAGPGGGSADKPI
                     GLVCFGLARTGKPAFSERQVFPGDRGAIRAATVAHAFQMIRAAL"
BASE COUNT          814 a         1329 c         1387 g          708 t
ORIGIN      
        1 gacaaactat ctggggtttt cgaccggctc cgcacgcgcg gatcgctgaa cgaggccgat
       61 gttaccgaag cgctgcggga agtccgtctg gcactgctgg atgccgacgt tgccctgccg
      121 gtggtgcgcg actttatcgc caaggttcgc gaacgtgcaa tcggggctga agtactgtcc
      181 tctgtcacgc cgggccaaca ggtcgtcaag atcgtcaacg acgtgatgat cgaggctttg
      241 ggcggcgcgg gcgcagtccc gatcaacctc aatgccgccg ctccggtgcc gatcctgatg
      301 gtcggcctgc agggctccgg caagaccacg acttcgggca agatcgcccg ccggctgaag
      361 gatcgcctga agcggcgtgt gctgctggcc agcctcgaca cacagcgccc ggccgcccag
      421 ttgcagctgg cgcagttggc cgaacaggct ggcgttccca gcctgccgat cgtcgccggc
      481 cagaccccgg tgcagattgc aacccgcgcc atggatgtcg gccggcgcga gggtttcgac
      541 gtcgtcatcc tggataccgc cgggcgtctg tccatcgacc aggaactaat ggacgaggtc
      601 cgccaggttc gcgccgtcac caatccggcg gaaacgctgc tggtggtcga tgctatgacc
      661 ggccaggatg ccgtcaacac cgccaaggcc ttcaacgacg cgctttccat taccggcatc
      721 gtccttaccc gtatggacgg cgatgcccgc ggcggtgcgg ctttgtccat gcgcgccatt
      781 accggcgccc cgatcaagct gatcggcgtc ggcgagaaac aggacgcgct ggaggatttc
      841 caccccgaac gcgttgccgg ccgcattctc ggcatgggcg acatcgtcgg cctggtggaa
      901 cgagcgacgg ataccattga tcaggcggaa gctgaaaagc ttgccgccaa gatgatgaag
      961 gggaagttcg atctggagga ttatgccagc cagcttcgtc agatcagcaa gatgggcagt
     1021 ctttccggta tccttgggat gttgccgggg gccggcaaga tcaagcagca gcttgaaggt
     1081 gccgatctgg acacgaaagt cctcaagcgt caggcggcga tcatctcgtc catgacgatc
     1141 aaggaacggc gcaccccgga tatcatcaag gccaaccgca agaaacgcat cgccgctggg
     1201 tctgggacga cggtccagga tgtcaaccga ctgctgaaac agttcgacga catgtccacg
     1261 atgatgaaga aaatgagcaa actgggccaa aagggcctga tgcgccatgg cctaggagcg
     1321 ctgatgccga aaggtgcgcc gcagcagcac cgccggccgt tctgacacga caatttcgaa
     1381 tacagcagga gatctcgtta aatgggcctg aaaatccgcc tcgcccgcgc cggtgccaag
     1441 aagcgtccgt actaccacat cgtcatcgcc gacagccgca gcccacgcga cggccgcttt
     1501 atcgagcgtg tcggcagcta caacccaatg ctgccagccg aacatgccga tcgcgttcgc
     1561 ctgcaaccgg agcgcctggc gcactggatc ggcaacggtg ccgtggcgac cgaccgcgta
     1621 gcaaaattcc tgggcaaagc tgggttggcg ccaatgccgg tatggaccga gcagccgcaa
     1681 cagtcggcgc cgaagaagaa ggcgcaggaa cgcgccaagg ccgtcgcggc tgcctctgcc
     1741 gcggcatgaa actgagccgc ggcatgagag actgaccagt gcctgacagt caccgtccag
     1801 caggccagat cccgccggga cgcatccagt tgggcgtcgt cggtcgggcg catggcgtgc
     1861 gggggttggt aaggatcacc agccacaccg ccgcacccgc cgacctgacc gcgtatggac
     1921 ctctttccga cccggagggc cgactttaca tcgtgtcctg gaagggcgag ggcattgccg
     1981 agattgctcg catgatcgac ggcgtcgcgg tgaaggtgac cgaccgtgca caagccgaga
     2041 agctgaccaa caccaagctg ttcgtcgatc gtgcggcgct gccgccggct ggcgaggacg
     2101 aattctacct gacggacctg atcggcatgg ccgccatcca ttcggacgga gccccgatcg
     2161 gaacggtttc cgccgtgcat gactacggcg cgggagccag tctggagatc accgggtccg
     2221 atgggccggt gattgttccg tttaccgccg cctgcgtgcc gatggtggac atcgccgccg
     2281 gacggctggt cgtcacgatg ccggatgaga tcgaggtgca tccagacacc tcccgcgacg
     2341 cggaggaagc ggcatgacct ggcgcgcctc tgtcctgacg ttgtttccgg ccatgttccc
     2401 cggtccgctg ggtcagtcct tggccggacg agcattggaa aatgcggtgt ggtcgttgag
     2461 cgtgaccaac atccggaact tcgcgcaaga tcggcaccgg accgtggacg acacgccatt
     2521 cggcggcggt gccggcatgg taatgcggcc ggatatcgtc gatgccgccg tgcagtcggt
     2581 ggcagacgat cggccggtgg tgtacctgac tccccgtggc cggcgcttca cccaggcgca
     2641 ggctcggcat ttcgccggag gaccgggcgt gatcctgctg tgtggccgtt atgaaggcat
     2701 cggccagcgg gttgtcgagg cgcgcggaat gcaggagctc agcatcggcg actacgtgct
     2761 gtcaggcggt gaattggctg cattggtcct gctggacagc gtcgtacggc tgttgccggg
     2821 cgtcatgggc gcggccgaaa gcgccgctga agaaagcttt tccggcggtc tgctggaata
     2881 tccccattac acccgcccgg ccgaatggca ggggcgccgc gtgccggacg tgctgctgtc
     2941 cggtcatcac ggcgccgtct ccgtttggcg tcaggcggaa tcagaacgca tcacccgtga
     3001 acgtcggccc gatctgtggc cctcggcagc gattccatag agaaggcaaa aggataaagc
     3061 catgaacatc attcagcagt tcgaggcgga gcaggtcgcc cgcttgacgg ccacgcgagc
     3121 cgttccggcg ttcatacccg gcgatacatt gcgggtttcc gtgaaggtcg tcgaaggcga
     3181 acggtctcgc gtccaggcgt tcgagggcgt gtgcatcgcc cgttccaaca agggcctgaa
     3241 cagcaacttc accgtgcgca agatcagtta tggcgagggc gtggagcgcg tgtttccgct
     3301 atatgcgccg acgatcgccg agatcgccgt ggttcgccgc ggcgatgtgc gccgtgccaa
     3361 actgtattac ctgcgtggac gcagcggcaa atcggcgcgt atcgcagaac gggctcgttc
     3421 ggctaccccg gttgctccga ccgaaccggc ggcggctgag tagaaaccgg ccccgtccgg
     3481 gaccgatcaa agagctgttg gcgtgcgaag cctacggtcg ctggcgatga agaaaggtca
     3541 tggaggcaaa ggcgcccaac catgacccga cccggcgaat gcgacatagg cccagttctg
     3601 cgtgtagctg atgacggcga aagacgagag cagataccac acgctgctcc aggttgccgc
     3661 gggcagccgc cggcgcgcgg caatggccga cgaaaacatg acatagaccg catcggtggc
     3721 ggccgtcgcc agcaggactc cgacggcggt cagcggatcg atcgccagta gcgcagccgg
     3781 aatcggtatc cccatcataa ggcggcgcgg atcatctgaa acgcatgagc gaccgtagcc
     3841 gcccgtatcg caccgcgatc gccgggaaat acctgccgtt ccgaaaatgc cggcttgccg
     3901 gtgcgcgcga gcccgaagca gacgaggcct atgggcttgt cggcgctgcc gcccccagga
     3961 ccggcaaccc cagcaaccga aaccgcgatc gcggctcggg agcgggcgag tgctccatcg
     4021 gccatcgcaa ccgcgacctg ttcgctgacc gcgccatgac attcgatcag ttccatcggc
     4081 acgccgatca tctgattctt ggcttcattg gagtatggga cgaaacctcg atccagcaca
     4141 tcggacgatc cggcaagagc cgtaagcgcc gcggcgatca ggccgccggt gcagctttcc
     4201 gctatcgcga gcatgatgcc cttggacctg cacagggc
//
LOCUS       DASWQN010001181         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_380527, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001181 DASWQN010000000
VERSION     DASWQN010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380527"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..492)
                     /locus_tag="VGC82_20085"
     CDS             complement(37..492)
                     /locus_tag="VGC82_20085"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0425628.1"
                     /translation="MPESIPETATISPIAPPQPQAGSPPPPPPPVSDQAAAAPATASL
                     RVTFAPGQSDLSPDSATSIKHLTESAAQGDGATFNVLAYAPGKPDDPSTARRVSLSRA
                     MSVRNALVASGIPSARIFVRALGEQFGDGPPDRVDIGVTGAPSASATAR"
     gene            907..2097
                     /locus_tag="VGC82_20090"
     CDS             907..2097
                     /locus_tag="VGC82_20090"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0425629.1"
                     /translation="MNPVSVGPIGPIDIDPVARARDLGPEITAAADEIEGSQTIPEPL
                     LSHIHDARLARILLPRSAGGDELEPWTYLHAIEEISRHDGSVGWNLFVANSAALIAPF
                     IPLETAQAIFTDARAWVSWGPPNQYQAHSVPGGYRVSGEWHFASGSRQATWMGAHCPV
                     AEADGSLRLNRFGRPTVRTLLFRKQQTQPIQDWNTIGMRGTASEGYRVTDLFVPEAFS
                     GTREDPSLRRDPGPLYAFTMQGLYAVGVAAVAFGIARAMLDEFITLAAEKTPRGLRRL
                     ADSAVVQADVARREATLGSARAWLVEILKDVWANADDIAPINLDARVRVRLGCAHAIS
                     TAVEVADYVYKAAGTSAIFRGTPFERRFRDMHTLSQQIQSRDSHFQAVGNAMFNGDPD
                     GLFL"
BASE COUNT          375 a          687 c          732 g          377 t
ORIGIN      
        1 acagcaacat gcgaatcagg tagcccgatg ggcgggtcat cgtgcggtgg ccgaagctga
       61 cggcgcgccg gttacgccga tatccacgcg atcgggcggc ccgtccccga actgctcgcc
      121 caacgcacgg acgaaaatcc gcgccgatgg gatgccggag gcgacgaggg cgttacgtac
      181 actcatggct cgggacagcg aaacccgccg cgcggtggat gggtcgtcgg gcttgccggg
      241 tgcgtaggcc aaaacgttga aagtcgcccc atccccctga gccgccgact cggtcagatg
      301 cttgatcgac gtggcactgt cgggactgag gtcggattgt ccgggtgcga acgtcacgcg
      361 caggctggcg gtcgctggcg ccgcggccgc ttggtcggaa accggcggtg gaggcggtgg
      421 cggtgacccg gcctgcggct gcggcggcgc aatgggagag atcgttgccg tttcaggaat
      481 gctctcgggc atcgccggct gcgccgcagg cggtagattt gcggtcggtg gattcgtcgt
      541 tgggggattt gtcgtttgtg gatttgccgg tgacacgcgc gtcactgtgg ttggcgccgc
      601 gggtccggtc gagggcgcag gacgcgacgg gcttctgccg agaggacgtt cgggaagcgc
      661 ctgcaacgca tgcaagtcaa ccgtgacctg tgcgaaagca ggtgtcgatg cagtcaggat
      721 gaggaaactc aggaacgaca ttgggcgcat atgattgctt ggtagcggaa cacgctgttc
      781 cgttcaatga accatgccgt gcgtggcgtt cgttgttcag cgcgaatcct tccgtttttt
      841 cgtgacattg acaagttcaa tccgacgcgc cggtaatcgg ccgagcgaga cgcaggaaca
      901 tcccccatga accccgtcag tgtcggcccc atcggcccca tcgacatcga ccctgttgcc
      961 cgggcgcgcg atctagggcc ggaaattacc gcggcggcgg acgagatcga gggcagccag
     1021 acgattcccg aaccgctgct gagccatatc cacgacgcca ggctggccag gatcctgttg
     1081 ccgcggtcgg ccgggggcga cgaactcgaa ccctggacct acctgcacgc gatcgaggaa
     1141 atcagccgcc atgacggttc ggttgggtgg aacctgttcg tcgccaacag cgccgccctg
     1201 atcgcgccat tcattccgct ggaaaccgcg caggcgatct tcaccgacgc gcgggcctgg
     1261 gtgtcctggg gaccgcccaa ccaataccag gcgcattccg tgcccggagg ctaccgggtc
     1321 agcggcgagt ggcacttcgc cagcggcagc cgccaggcca cctggatggg agcacattgt
     1381 ccggtggccg aagcggatgg ctcgctgcgc ctcaatcgct tcgggcgccc cacggtccgg
     1441 acgctgctgt ttcgcaagca gcagacgcag ccgatccagg actggaacac gatcggcatg
     1501 cggggcaccg cgtccgaagg ctaccgggtc accgacctgt tcgtccccga agccttcagc
     1561 ggcacacggg aggatccgtc gctgcgccgc gacccgggac cattgtacgc cttcaccatg
     1621 caggggctct acgcagtcgg tgttgccgcg gtcgccttcg gcatcgccag ggcgatgctc
     1681 gacgagttca tcacattggc ggccgagaaa acgccgcgcg ggctgcggcg tctggcggac
     1741 agcgccgtcg tgcaggccga tgtcgctcgc agggaagcaa ccttggggtc tgcgcgggcc
     1801 tggctggtcg agatcctgaa ggacgtctgg gccaatgccg acgacatcgc cccgataaac
     1861 ctcgacgctc gggttcgggt ccggcttggc tgcgcgcacg cgatcagcac ggcggtcgag
     1921 gtcgcggatt acgtctataa agccgccggc acgtcggcga tcttccgcgg caccccgttc
     1981 gaacgccgct tccgcgacat gcataccctt tcgcaacaga ttcagtcgcg agactcgcat
     2041 tttcaggccg tcggaaacgc catgttcaac ggcgatccgg atgggctatt cctataggat
     2101 cgcagaccgg tccatttgct gacctgaaca ccatatccgc ccaggcgatc ccgtctggat
     2161 ggaccttgct c
//
LOCUS       DASWQN010001182         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_116187, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001182 DASWQN010000000
VERSION     DASWQN010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116187"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VGC82_20095"
     CDS             <1..515
                     /locus_tag="VGC82_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEY0425630.1"
                     /translation="IQAGVTISTHLGNALPQPQPKFLNPLMAQLAADALYASFIADGI
                     HIPPEALKVLLRGKTPRWSVLVTDATAAAAAPPGLYVFAGMTIQHQADGSVRLPNTAT
                     LAGSALTLDQAVRNLVAWKLADATTALAMASTHPNAVLAPALAYHRITLPTARVAWTA
                     DLHPTQFLSE"
     gene            661..1935
                     /locus_tag="VGC82_20100"
     CDS             661..1935
                     /locus_tag="VGC82_20100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0425631.1"
                     /translation="MLTAHATAPAARSRLPVATEPACRLCGSRLHRSLIDLGCQPLAN
                     RTIAAGAPDGPSYSLHARICDDCKLVQLSDVAPAAILAAETPSLSSKSPARLMQARRY
                     ADIACKRLKLDGNALVIEIGSNDGYLLRHFHAAGIPVLGIECAPIAATATMELGIPTE
                     TGFFAAESAMEIAVRHGRADLVVANNVLQQVPDLFDFAAGFASILRPNGVLMLQVPHL
                     LSLLQRVQFDAFRHDTYAYLSLQVLERILRSVGLRIFDAERIPDHGGSLRVQACHAVS
                     AIATKPGLKSVRMAEAFAELERPDLFTGFSAVVAAACREIRDFLHIRRTAGRRVAAYG
                     AATRGCSMLNACGVTTAEIACVADPDPAMHGRFLPGSRIPIVSLEVLLAEPPDDILVL
                     PWTNTAEVAAPLHPLRQRGTSLWTAIPRIARL"
     gene            1922..>2367
                     /locus_tag="VGC82_20105"
     CDS             1922..>2367
                     /locus_tag="VGC82_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425632.1"
                     /translation="MPGYDKRAGASSEVARSAIVWYRDRYVTGTWNMASRDSALARAL
                     THFDSDGFRDHLAELVAIPSTSQDPGHEADVHRYLASAIQPWLERMGFTIEIHPNPET
                     GFGPILFAERLEGPDRPTVLTYGHGDTVRGLDDQWREGLSPWTLTE"
BASE COUNT          382 a          885 c          694 g          406 t
ORIGIN      
        1 cgattcaggc cggcgttacg atcagcacac atctcggcaa cgccctgccg cagccgcagc
       61 caaaattcct caacccgctg atggcccagc tcgccgccga cgcgctgtac gccagtttca
      121 tcgccgacgg catccacatc ccgccggaag cactcaaggt cctgctccga ggcaagaccc
      181 cgcgctggtc ggtcctggtc accgatgcaa cggccgccgc cgccgcgcca ccgggtctat
      241 atgtcttcgc tggcatgacc atccagcacc aagcggatgg ttcggtccgg ttgccgaata
      301 ccgccacgct ggccggttcg gcgctgactc tcgatcaggc ggtgcgcaac cttgtggcct
      361 ggaagctggc cgatgccacc actgcgcttg ccatggcgag cacccacccc aacgccgtgc
      421 tggcccctgc cctggcctat caccgcatca ccttgcccac ggcccgggtg gcctggaccg
      481 cagatttgca tccgacgcag tttctttcgg aatagaaact tcctatttac caatcccgcg
      541 ctaatctcgt ggcagcgaga aacgcgacct cgtcaggtac ggtggacaga acggccattc
      601 cctggcttcc cgatatggga aaggcctgtc cgccaccgtg acgaaccgat cgagcaggcg
      661 atgctgaccg cgcacgccac cgcccccgcc gcccgttcca ggctgccggt ggcaaccgaa
      721 cccgcctgcc ggctctgcgg cagccggctg caccgctccc ttatcgacct cggctgccag
      781 ccgctggcca accgcactat cgccgccggc gcaccggacg gtcccagcta cagcctgcat
      841 gcccgcatct gcgatgactg caagctggtt cagctgtcgg atgtggcgcc cgccgcgatc
      901 cttgccgccg agacgccctc cctgtcgtca aaatcccccg ctcgcctcat gcaggctcgg
      961 cgatacgccg acatcgcgtg caagcgcctc aagctggacg gcaacgcgct ggtgatcgag
     1021 atcggctcca acgacggcta cctgttgcgt catttccacg ccgccggcat cccggtgctc
     1081 gggatcgagt gtgccccgat cgccgccacc gccaccatgg agctgggcat tcccaccgaa
     1141 accggcttct ttgccgccga atcggcgatg gagatcgccg tgcgtcacgg ccgtgcggac
     1201 ctggttgtag cgaacaacgt gctgcagcag gtgccggacc tgttcgactt cgccgccggc
     1261 ttcgccagca tcctgcggcc gaacggtgtg ctgatgcttc aggtgccgca tctgctgtcg
     1321 ctgctgcagc gcgtgcagtt cgacgccttc cgccacgaca cctacgcata cctttcgctg
     1381 caagtgctgg agcgtatcct ccggtcggtc gggctacgga tattcgatgc cgaacgcatt
     1441 cccgaccacg gcgggtcctt gcgcgttcaa gcctgccacg cggtctctgc gattgcgacc
     1501 aaacccgggc tgaaatccgt ccgaatggcg gaagcgttcg ccgagctgga gcgtccggac
     1561 ctgttcaccg gcttcagcgc cgtcgtggcc gccgcctgcc gcgagatccg ggatttcctg
     1621 catatccgcc gcaccgcggg gcgccgggtc gcggcctatg gcgccgccac aagaggctgc
     1681 tcaatgctca acgcttgcgg cgtcaccacg gccgagatcg cctgcgtcgc cgatcccgac
     1741 ccggcgatgc acggccgctt tttgcccggc agccgtatcc cgatcgtctc cctcgaagtg
     1801 ctgctggcgg aaccgccgga cgacatcctt gttctgccct ggaccaacac tgcggaggtc
     1861 gcagccccgc tgcatcccct ccgacagcga ggcacctcgc tttggacagc catcccgcgg
     1921 attgcccggc tatgacaagc gggccggagc gtcgtccgag gtagcccgga gcgccatcgt
     1981 ctggtaccgt gaccgctacg ttactgggac ctggaacatg gcatcgcggg acagcgccct
     2041 ggcgcgcgca ctgacacact tcgactcgga cggctttcgc gaccacctcg cggagctggt
     2101 tgccatcccc agcacttcgc aagatcctgg ccacgaggcc gacgttcacc gctatcttgc
     2161 ctccgccatc cagccctggc tggagcggat ggggttcacc atcgagatcc atcccaaccc
     2221 ggaaaccggc ttcggcccca tcctgttcgc cgaacggctg gaaggtccag accgcccgac
     2281 cgtcctcacc tacggccacg gcgacaccgt ccgcggactg gacgaccagt ggcgcgaggg
     2341 cctttcgccc tggacgctga ccgagga
//
LOCUS       DASWQN010001183         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_380631, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001183 DASWQN010000000
VERSION     DASWQN010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380631"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1322
                     /locus_tag="VGC82_20110"
     CDS             288..1322
                     /locus_tag="VGC82_20110"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0425633.1"
                     /translation="MMPDEPGKLPVSVVTGFLGSGKTTLISRVLRDPAFARTAVIVNE
                     FGEIGLDHNLIATSDDSVVTLNTGCLCCVVQTDLNRTLIELLERREAGTADYDRVLIE
                     TSGLADPAPVLQGMMTDGAIAATHHIPRVVTIIDTIFGGVNLRDHNEARHQIALADRI
                     LISKTDLQPASDQLLAELDALNPGALRMSTTQAEADNLFADPSFDALTERLARLVRQP
                     ARHAGIDTFSVIRDKPLPALALTLLLQAVAEHCGPRLLRLKGLVAIAEMPGRPAVIHG
                     VRHVVSAPEFLDRWPTGDEQTRIVFITKGVPRYFVARLLDAIVHEVHEETATRGSSSS
                     AVVMPSKQAN"
     gene            1390..2958
                     /locus_tag="VGC82_20115"
     CDS             1390..2958
                     /locus_tag="VGC82_20115"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0425634.1"
                     /translation="MKRRSLLAGAAASLARPSLAGSSKTLTIIPQVALNSIDPVWTSS
                     QIARNMGYMVFDQLYGRDEAMNARPQMVEADLMEDGARRWTLRLRENLWWHDGEKVLA
                     RDCVASLKRWMKRNPAGGALEARLDAIEVLDDRSFVLRLNKPYPHLRMLLSQFVIPPV
                     MMPERLAATDPFKQIPEAIGSGPFRWLADEHVLASHAAFARFDRYVPRDEPASYTAGG
                     HRVLLDRVEWKMIPDPQTAANALITGEVDWMEIPLPDLIPLLKKTPNVVTGNLDSYGQ
                     ILFMRPNHTLSPTNNPAIRQAMMAAVDQREVMAAVMGGDPENAIAPIGFLQSGKPEVD
                     QAGMEAIRVRHTPQEVKAMLDKAGYKGERLVLLHPTDHIYYNPTTAVVSQLLAECGFN
                     IDDQTMDWGTVQSRRTSREPLTNGGWSIFCTVVPMPDYRDPLLAAFIRGNGAKGWFGW
                     AEDARMEQLYDTWLETTDPAEQTRLEREYQLQAFKFLPFIPLGRYLQTSAWRDNVHGI
                     PKGPSAVFWNIEKT"
BASE COUNT          577 a          927 c          954 g          534 t
ORIGIN      
        1 gcacgccgcc ccgggggaga tgatgccgct gaccatggac ctgaaccaga tgcacctgat
       61 ggaagccgcc agcggcaggg tggtctgagc gggagctggg gaggcctacg agtactgata
      121 gtgttctcgc agtgcccaga cccagggcag cgatcggttg cttttggatc ttaagacgct
      181 tcgtcgtcgc catgcaggcc tgagccaaag cagcaacgcc agcgccgaaa cgcctgaagg
      241 gcccaggact tgggtctaaa cgggcccagg tgagcgagta ccggcccatg atgccagacg
      301 agccaggcaa gctcccggtt tcggtcgtga ccggctttct gggcagcggc aagaccacgc
      361 tcataagccg cgtcctgcgt gatcccgcgt ttgcccgaac cgctgtcatc gtcaacgaat
      421 tcggtgagat tggcctggat cacaacctga tcgccaccag cgacgattcg gtcgtgacct
      481 tgaatacggg gtgcctgtgc tgcgtggttc agactgatct caatcgcacc ttgatcgaac
      541 ttctggaacg ccgtgaggcc ggaacggcag actatgatcg cgtgctgatc gagacctcgg
      601 ggctggccga tcccgcgccg gtgttgcagg gcatgatgac cgacggcgcc atcgccgcga
      661 cccatcacat cccccgagtc gtcacgatta tcgacaccat cttcggcgga gtaaaccttc
      721 gggaccacaa tgaggctcgg catcagatcg cgctcgcgga tcggattctc atcagcaaaa
      781 ccgatctgca accggcatcg gaccagttgc tcgcggaact ggatgcgttg aatccagggg
      841 ccttgcgtat gagcacgacg caggccgaag ccgacaatct gttcgccgat ccgtccttcg
      901 acgctttgac ggaacgtctg gctcgtttgg tccgacagcc ggcgcgccat gctgggatag
      961 acacgttttc cgttatacgg gacaaacctt tgccggcgct cgcgctgacc ttgcttttgc
     1021 aagcggtcgc ggaacattgc gggccgcggc tgctacgcct gaaaggcctt gtcgcgattg
     1081 cagagatgcc gggtcggccc gcggtgatcc acggcgttcg ccacgtcgtc agtgcgccgg
     1141 agtttctcga ccggtggccg acgggggatg aacaaacccg catcgttttc attacaaaag
     1201 gcgtaccgcg gtatttcgtt gcacgcctgc tggatgcgat cgtccatgag gtacacgagg
     1261 agacggccac gcgtggttcg tcgtcgtcgg ctgttgtgat gccgagcaag caggcaaact
     1321 gaccacaggc atctgcctat cgacttcgga cccttctggc ggctagcctc gcgacggacg
     1381 gggggaacga tgaaacgacg cagtcttttg gccggagccg cggcgtcgct cgcacgtccc
     1441 tcgctcgccg gcagttccaa aacgctgacc atcatccccc aggtcgcgct caacagcatc
     1501 gatccggtat ggaccagctc gcagatcgcc cgaaacatgg gctacatggt gttcgaccag
     1561 ctctacggcc gtgatgaggc catgaacgcg aggcctcaga tggtcgaggc cgacctgatg
     1621 gaggacggcg cgcggcgctg gacgttgcgg ctacgggaaa acctgtggtg gcacgatggc
     1681 gagaaagttc tcgcgcgcga ctgcgtcgct tcgctgaagc ggtggatgaa gcgcaacccg
     1741 gccggcggcg cgctggaggc aaggctcgat gccatcgagg tgctcgacga ccgcagcttc
     1801 gtgctgcggc tgaacaagcc atatccgcat ctgcgcatgt tgctttcgca gttcgtcatc
     1861 ccgccggtga tgatgccgga acgtctggcc gcgaccgatc cgttcaagca gatccccgaa
     1921 gcaatcggca gcgggccgtt ccgctggcta gcggatgaac atgtgttggc cagccacgcc
     1981 gcattcgccc gcttcgaccg ctatgtgccg cgcgacgaac cggccagcta caccgcgggc
     2041 gggcatcgcg tgctgctcga ccgggtggaa tggaagatga tccccgaccc gcagaccgcc
     2101 gccaacgccc tgatcacagg tgaggtggac tggatggaaa tcccgctgcc ggacttgatt
     2161 ccattgctga aaaagacccc gaacgtggtc accggcaatc tcgattccta cgggcagatc
     2221 ctgttcatgc ggccgaacca cacgctgtcg ccgaccaaca acccggcgat ccgccaggcg
     2281 atgatggcgg cggttgacca gcgggaggtg atggccgcgg tgatgggcgg cgacccggag
     2341 aatgccattg ccccgatcgg cttcctgcag tcgggaaagc cggaagtgga ccaggccggc
     2401 atggaagcga tccgcgtccg gcatacgccg caagaggtca aggcgatgct ggacaaggcc
     2461 ggatacaagg gcgagcggct ggtgctgctg catcccaccg accatatcta ttacaatccg
     2521 acaaccgccg tcgtgtcgca gttgctggcc gaatgcggct tcaacatcga cgaccagacg
     2581 atggactggg gcacggtgca gtcgcgacgc accagccgcg agccgttgac gaacggtggc
     2641 tggtcgatct tctgcactgt cgttccgatg ccggattacc gcgacccgct gctcgccgcg
     2701 ttcatccgcg gcaatggcgc caagggctgg ttcggctggg ccgaggatgc gcgcatggag
     2761 cagctttacg atacctggct ggaaaccacc gacccggcgg aacaaacccg gctggagcgc
     2821 gaataccagt tgcaggcgtt caagttcctg ccgttcatcc cgctcgggcg gtatctgcag
     2881 acctcggcct ggcgtgataa tgtacatggc atcccgaaag gtccgtccgc ggtgttctgg
     2941 aatatcgaga agacctgaca gcttgcgcga aacggtgcgc acccataagc ac
//
LOCUS       DASWQN010001184         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1039470, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001184 DASWQN010000000
VERSION     DASWQN010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039470"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VGC82_20120"
     CDS             <1..282
                     /locus_tag="VGC82_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425635.1"
                     /translation="AVATELPARQSVTAADAGAGVRNDLQQWGDQVSHQAFVDQAAAT
                     IVYQVIDSRTAMVVEQFPDEAVLRRRAYFHALDLTKDTPARRLATDRKA"
     gene            complement(290..652)
                     /locus_tag="VGC82_20125"
     CDS             complement(290..652)
                     /locus_tag="VGC82_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425636.1"
                     /translation="MADLMSILSANYKTTGAAVPSRTKYVAVDPALYEGKWTGTYADK
                     KKFTLTVTDVSGFRAQVKYESGGTIKNSSVLIKDASFRIGDTKFTWAKAGTGLVKTVV
                     TDPATGSTYLDTAAATQR"
     gene            complement(680..1045)
                     /gene="flaF"
                     /locus_tag="VGC82_20130"
     CDS             complement(680..1045)
                     /gene="flaF"
                     /locus_tag="VGC82_20130"
                     /inference="COORDINATES: protein motif:HMM:NF009435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HEY0425637.1"
                     /translation="MSQAAQAYARTSHATAHPREIEAQALLKAARQLQEVQANWSGPD
                     STMHNALLFNRRLWSIFMSAAETNENPQPMEIRQNIANIGVFVMKQTLEMQVNPDPAK
                     LKPLIDINCNLAAGLSGRG"
     gene            complement(1263..>1730)
                     /locus_tag="VGC82_20135"
     CDS             complement(1263..>1730)
                     /locus_tag="VGC82_20135"
                     /inference="COORDINATES: protein motif:HMM:NF012903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY0425638.1"
                     /translation="IAQITTTAQDASFNGINLLNGDVLKLTFNETGKSTLSITGVTYN
                     STGLGLNTLATGTDFLDNNTTNKVITALGAASTSLRTEASALGSNLSIVQIRQDFSKS
                     LINVLQTGSSNLTLADTNEEAANSQALSTRQSIAVSALSLSNQAQQSVLQLLR"
BASE COUNT          274 a          518 c          568 g          370 t
ORIGIN      
        1 gcggtcgcga ccgaactgcc cgcgcgccag agcgtcacgg cggcggacgc cggcgcgggc
       61 gtgcgcaacg acttgcagca atggggtgac caggtgtcgc atcaggcgtt cgtcgatcag
      121 gccgccgcga ccatcgtcta ccaggtcatc gacagccgca ccgcgatggt ggtggagcaa
      181 tttcccgacg aggcggtgct gcgccgccgg gcctatttcc acgccctgga tctgaccaag
      241 gacacgccgg cgcggcgtct tgccaccgac cgcaaggcgt gagggccgtc tagcgctgag
      301 tggcggctgc ggtgtcgagg taggtcgatc cggtggccgg atcggtgacg acggtcttga
      361 ccagccccgt tccggccttg gcccaggtaa acttggtatc gccgatccgg aacgaggcgt
      421 ccttgatcag gacgctggaa ttcttgatcg tgccgccgct ctcatacttc acctgcgcgc
      481 ggaagccgct gacgtcggtc accgtcaggg tgaacttctt cttgtcggcg taggtgccgg
      541 tccacttgcc ctcgtagagc gccggatcga ccgccacata cttggtccgg gacggaaccg
      601 ccgccccggt ggtcttgtag ttggctgaca ggatgctcat gaggtcggcc atgacagcct
      661 ccaatggcgg aacggcgttt cagccccggc ccgacaggcc ggcggcgagg ttgcagttga
      721 tgtcgatcag cggcttcagc ttcgccggat ccgggttcac ctgcatttcc agcgtctgct
      781 tcatcacgaa cacaccgata ttggcgatgt tctggcgaat ttccatcggc tggggatttt
      841 cgttggtctc ggccgcgctc atgaagatcg accacagccg gcgattgaac agcagcgcgt
      901 tgtgcatggt gctgtccgga ccgctccagt tggcctggac ctcctgcagc tgccgtgccg
      961 ccttcagcag cgcctgtgcc tcgatttccc ggggatgggc cgttgcgtgc gaggtgcgag
     1021 cataggcctg agcagcttga gacattcgtt tcctcgacag cggttcttgg gcaggaatca
     1081 gccccttggg ctgcccaagt tttgtacgtc ccggccttta aaatatggtt accaaacaat
     1141 tccgtatttg tccggaacct ggcagccaag gcgcgcgtgg cccttgcccc cctcagttca
     1201 caaggagaga acggcggggt tttgccccgc cgtttgcttt ggctcttttg ctttttcgaa
     1261 gatcagcgca gcagctgcag cacgctctgc tgggcctggt tggagagtga cagcgcggag
     1321 accgcgatcg actgtcgggt cgacaacgcc tggctgttgg ccgcttcctc attggtgtcg
     1381 gccagcgtca ggttggacga gccggtctgc agcacgttga tcaggctttt cgagaagtcc
     1441 tgccggatct gaacgatcga caggttcgag cccaaggccg acgcttcggt ccgcagcgag
     1501 gtgctggccg caccaagcgc ggtgatgacc ttgttggtcg tgttgttgtc gaggaagtcg
     1561 gtccccgttg ccagcgtatt caggcccaga ccggttgaat tgtaggtaac gccggtgatg
     1621 ctgagcgtgg acttgccggt ttcgttgaag gtcagcttga ggacgtcgcc gttcagcagg
     1681 ttgatgccgt tgaacgatgc gtcctgcgcc gtggtggtga tctgcgcaat
//
LOCUS       DASWQN010001185         2415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1699253, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001185 DASWQN010000000
VERSION     DASWQN010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699253"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VGC82_20140"
     CDS             <1..640
                     /locus_tag="VGC82_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0425639.1"
                     /translation="GDIGQKDFDRFVQVSREISGLPIQIDDTPAITMSALRTRCRRLK
                     RTKGLALVIVDYLQLMRPAAGTKPESRVLEISMITQGLKALAKELAIPVMALSQLSRQ
                     VENRDDKRPQLSDLRESGSIEQDADAVMFVYRDEYYLEQKMPKEVAFEGSADKFNAAM
                     DEWQQRMAKAHNRADLIIAKQRHGPTGSIPLLFEGEFTRFGDIDLVHSDYAG"
     gene            630..1703
                     /gene="alr"
                     /locus_tag="VGC82_20145"
     CDS             630..1703
                     /gene="alr"
                     /locus_tag="VGC82_20145"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY0425640.1"
                     /translation="MPGETAQAVLEVDLDAIGANWRSLCARHPSGPVAGVIKADGYGL
                     GARPVAAALHKAGCRHFFVAYLNEALAVRDVVPGSMLAVLSGLIPGTEDVYVSHDLTP
                     VLGSLDEIERWRGKGCDAILHIDTGMSRLGFDARELDVLASESSRLDGLPIRYVMSHL
                     AASEVPGDPSNQLQRERFRTARAMLPHAPGSLANSSGIFLGDDFASDLARPGAALYGI
                     NPTPGQPNPMRAAVRLSVRVLAVRDVPAGAGVGYNATWIAPRPSRIATAALGYADGFH
                     RSLSGRGSASFDGTPVPLVGRVSMDLTTFDVTAHPGVQPGCWLEVLGPHLSPDDLAEA
                     AGTNGYEVLTSLGRRFHRVYRTA"
     gene            1700..>2415
                     /locus_tag="VGC82_20150"
     CDS             1700..>2415
                     /locus_tag="VGC82_20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0425641.1"
                     /translation="MTLILDFLAAIGRPIIGLCRAAGALALFGLDGLSHLVRPPFYGR
                     LFLKALTEIGYFSLPVVALTAVFTGMVLALQSYTGFARFSAQGAVANLVVLSVTRELG
                     PVLAGLMVAGRVGAAMAAELGTMRVTDQIDALSTLSTHPMKYLVAPRLLAGIIALPLL
                     VLIADVLGVMGGFIVSTLKLGFNVSGYLTNTINFVQMEDVASGLVKAAVFGFLISLMG
                     CYQGYNSKGGAQGVGAATTLA"
BASE COUNT          409 a          725 c          809 g          472 t
ORIGIN      
        1 cggcgatatc gggcagaagg attttgacag gttcgtccag gtatcgcgtg agatctccgg
       61 cttgccgatc cagatcgatg atacgccggc gatcaccatg tcggcgctgc gcacacgctg
      121 ccgaaggttg aagcggacca agggactggc gctggtgatc gtcgattacc tgcagctcat
      181 gcgtcccgcc gcaggcacga agccggaaag ccgggtgctg gaaatcagca tgattaccca
      241 gggcctgaag gcgctggcga aggaactggc aattccggtg atggcgttgt cgcagctttc
      301 ccgccaggtg gaaaaccgtg acgacaagcg gccgcaatta tccgatctac gagaatccgg
      361 gtcgatcgaa caggacgccg acgcggtgat gttcgtctat cgcgacgaat actacctcga
      421 gcagaaaatg cccaaggaag tcgcgttcga aggtagcgcc gacaagttca acgcagcgat
      481 ggacgaatgg cagcagcgca tggcgaaagc ccataaccgc gccgacctga tcatcgccaa
      541 gcagcgtcat ggccccaccg ggtcgatccc gctgctgttc gagggcgagt tcacccggtt
      601 cggcgatatc gacctcgttc actccgatta tgccgggtga aaccgctcag gcggtcctcg
      661 aagtcgatct ggacgcgatc ggggccaact ggcgatcctt gtgtgcgcgc catccgtccg
      721 gcccggtggc cggtgtcatc aaagccgatg gctacggcct aggtgcgcga cctgtggctg
      781 ccgcactaca taaagccggg tgccggcatt tctttgtggc ctatttgaac gaagcgctgg
      841 cggttcgcga cgttgtgccg gggtcgatgc ttgcggtcct gagcggcctg ataccaggaa
      901 ccgaggacgt ctatgtgtca cacgatctga caccggtcct aggttcgttg gacgaaatcg
      961 agcgctggcg gggcaagggt tgcgacgcca tcctgcatat agacaccggg atgtcccggc
     1021 tgggcttcga tgcacgggaa ctggacgttt tggccagcga gagtagccgc ctggatgggc
     1081 tgccgatccg ctatgtgatg tcgcatctgg ccgcttccga agtgcccggc gacccatcga
     1141 atcaactcca gcgggagcgg ttccggacag cccgggcgat gctgccgcat gcgcccggca
     1201 gcttggcaaa ctcctccggc attttcctcg gcgacgattt cgcatcggac ctggctcggc
     1261 cgggtgccgc gctgtatggc atcaatccaa cgcccggcca gcccaatcca atgcgtgccg
     1321 cggtgcgttt gtcggtccgg gttctggcgg ttcgcgatgt cccggccgga gctggcgtcg
     1381 gctacaacgc cacctggatc gccccgcgcc caagccgcat tgccacggcg gcgctgggct
     1441 atgcggacgg tttccaccga agcctgtcag ggcgcggatc ggcgagtttt gacggcaccc
     1501 ccgttccgct ggtaggccgg gtatccatgg accttacgac attcgacgta accgcccacc
     1561 ccggcgtgca acccggttgc tggctggagg ttcttggccc gcatctgtct cctgacgatc
     1621 ttgccgaagc ggccggcacg aacgggtatg aggtgctgac gtctctgggc cgccgcttcc
     1681 atcgggtgta tcgaacagcg tgacattgat cctggacttc ctggcggcta ttggccgacc
     1741 gatcatcggc ttgtgccgag cggcaggcgc gctggcgctt ttcgggctgg acgggctatc
     1801 gcacctggtc cggccgccgt tctacggacg gctgttcctg aaggcgctga cggagatcgg
     1861 ctatttcagc ctgccggtgg tggcgctgac cgcggtgttc accgggatgg tgctggcgct
     1921 tcagtcatat actggctttg cccggttctc ggctcagggc gccgtggcga atctggtggt
     1981 actttcagtg acgcgggaac tcgggccggt gctggccggg ctgatggtgg ccgggcgggt
     2041 aggcgctgcg atggcggccg aactcggcac gatgcgggtc accgaccaga tcgatgcgct
     2101 ttcaacgctt tccacccacc cgatgaaata tctcgtggca cctcggctgc tggcggggat
     2161 catcgcattg ccgctgctgg tgctgatcgc cgacgtgctg ggggtgatgg gcgggttcat
     2221 cgtgtcaacg ctgaagctgg ggttcaacgt cagcggctac ctgaccaaca cgatcaactt
     2281 cgtgcagatg gaagacgtgg cgtcagggct ggtgaaggcg gccgtgtttg ggttcctgat
     2341 ttccctgatg ggctgctacc agggctacaa cagcaaaggc ggcgcgcagg gcgtgggagc
     2401 ggcgacgact ctcgc
//
LOCUS       DASWQN010001186         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2096042, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001186 DASWQN010000000
VERSION     DASWQN010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2096042"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..1485)
                     /locus_tag="VGC82_20155"
     CDS             complement(325..1485)
                     /locus_tag="VGC82_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEY0425642.1"
                     /translation="MFIREQRFSINQVRQVLGGVLNQDLHAKRVDSLCDATLGVLRSA
                     SLAVCTIGQGLAAARGLNPKHATKQVDRLLSNPKINVDDILARWVPYIVGARSSIVVA
                     LDWTDFDADKQATIMLSLITDHGRATPLVWLTVDKRALKDNRSLYEHRVLVRLAELLP
                     ADVKVCVVADRGFGDQKLYRMLTEELCFDYIIRFRGNITVTATTGETRTAAAWVRASG
                     RARVLRGAAVTADRYAVGTVVCVQDPGMKQAWCLAASSTNATAKHLTEYYGRRWGIEC
                     ALRDTKDLRFGMGMGAVHVNSPKRRDRLWLINAFAVVLLTLLGAAGEALGYDRMLKTN
                     TAKRRVHSLFRQGCMLYDLIPTMPETRLRPLMQHFSQMLQEQPLFEDVFGPV"
     gene            complement(1751..2467)
                     /locus_tag="VGC82_20160"
     CDS             complement(1751..2467)
                     /locus_tag="VGC82_20160"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEY0425643.1"
                     /translation="MLTRLVAKGAERSNGRSAGHGRQRRGDTGVEHQAVRQDLPKDGQ
                     GAVKTLSQPIAWNFSQIPIYLADPTGGGKPASTATTAPAIAREVLRAPGTSLDAATRS
                     YFEPSFGFDFSRVRVHADPLAAAAARSVRARAFTVGRDIVFAADRFAPGTREGQRLLA
                     HELTHVVQQTMVPNDAASAADAEVEAARAGTACLAGLREGAGKRAGGGQSRGAPGAGA
                     ESVGAAVPMPMQAMLSPLCT"
BASE COUNT          487 a          880 c          851 g          513 t
ORIGIN      
        1 cgatagatcg tgaagtgagc cccgccgcca tccctcttaa agacgtggca ctctacggcg
       61 gtcttaccga ccactacctc atccaccatg ccatcggtct tggtaggagt gatttgtgct
      121 agtgcctgcc ggtggcactt cctcaacgcc gtgcgtaggg atgcaggcgt gtcatcggtc
      181 acggctgctc cagcctttac agcaacgtca aaaaactggt caggcggcga ggccccgttc
      241 caacttgcat tgacatccat gccgccgacg aagcgcgatc ctgatttagc ctcttcatcg
      301 taccctcatg actcccctca tttatcagac cggcccgaaa acgtcttcga agagcggttg
      361 ctcctgtagc atttgagaga agtgctgcat cagcgggcgc agcctggttt ccggcatcgt
      421 cgggatcaga tcgtagagca tgcagccttg gcggaacaac gaatggacac ggcgcttggc
      481 tgtgttggtc ttgagcatcc ggtcatagcc cagcgcctcg ccggccgcgc cgagcaacgt
      541 caacagcacc accgcgaagg cgttgatcag ccataaccgg tcgcgtcgct tgggggaatt
      601 cacgtgcacc gcgcccatcc ccatgccgaa gcgcaggtcc ttggtatcgc gcagtgcgca
      661 ctcaatgccc cagcggcggc cgtagtattc ggtcaggtgc ttggccgtgg cgttggtgct
      721 gctggcggcc aggcaccagg cttgtttcat gcccgggtcc tgcacgcaga ccacggttcc
      781 cacggcgtag cggtcggcgg tgaccgcggc gccgcgcagc acacgggcgc gtccgctggc
      841 ccgcacccag gcggcagcgg tgcgggtttc gccggtcgtg gcggtgacgg tgatgttgcc
      901 gcggaagcgg atgatgtagt cgaaacacag ctcctcggtg agcatccggt agagcttctg
      961 gtcgccgaag ccgcggtcgg cgaccacgca caccttgacg tcggccggca gcagttcggc
     1021 cagccgtacc agcacacgat gctcgtagag gctgcggttg tccttgagcg cgcgcttgtc
     1081 gaccgtcagc cataccaggg gcgtggcacg accgtggtcg gtgatcaggg acagcatgat
     1141 ggtggcttgc ttgtcggcgt cgaagtcggt ccagtccaac gccaccacga tggagctacg
     1201 cgcgccgacg atgtagggca cccagcgagc gaggatatcg tcgacgttga tcttcgggtt
     1261 ggaaagcagt cggtcgacct gcttggtggc atgcttgggg ttgaggccgc gggcggcggc
     1321 gagcccttgg ccgatggtgc aaaccgcgag cgaggcgctg cgcagcacgc cgagggtggc
     1381 atcgcagagc gagtcgacgc gcttggcgtg aaggtcctgg ttcagcacgc cgccaaggac
     1441 ctgccgaacc tggttgatcg agaaccgctg ctctctgatg aacatcctat tcccccgacc
     1501 tgctgttgcg gggcgggaga tttgctggac caggcggcat tggaagtcgc cttggcgaat
     1561 tcgcaaggat atgccgccgg cgacggtcgt acgattcttt ccggaatcgc ccctacggtt
     1621 gcccgattgg gcgaatatct gccgacgcca aagaacgagg tggtcgcgga cctttatccc
     1681 cgactgggat tttcccgtgt cgagggaccg ttctttatgc gtgaaaccac cgccggaacc
     1741 gaggatctgg ttacgtacat aacggactca gcatagcctg catcggcatc ggaacggcgg
     1801 cgcctacaga ttcagcgccc gcaccaggtg cccctcggga ttggccgccc ccagcacgtt
     1861 tccccgcgcc ttcccgcagt ccggccagac aagcggtgcc cgcccgcgcc gcctcaacct
     1921 ccgcatccgc cgccgaggcg gcatcgttcg gcaccatcgt ctgttgcacc acatgcgtca
     1981 gctcgtgcgc cagcaaccgc tgtccctcgc gggtcccagg tgcaaatcgg tccgcggcaa
     2041 agacgatgtc ccggcccaca gtaaaagccc gagctcggac acttcgcgcc gccgccgcgg
     2101 caagcggatc agcatgcacg cgcacccggc tgaaatcgaa gccgaagctg ggctcgaagt
     2161 aggaacgcgt cgccgcgtcg agcgaggttc ctggtgcgcg cagcacctct cgcgcaatcg
     2221 ccggcgcggt cgtggcggtg gaggccggtt ttcctccccc agtgggatcg gccagataga
     2281 tggggatctg cgagaaattc cacgcaatcg gctgcgacaa ggtcttgacg gcgccttgcc
     2341 catccttcgg caagtcctga cgaacagctt ggtgttcaac gccagtatcg ccgcggcgct
     2401 gtcggccgtg gccggccgac cttccgttcg aacgttcagc gccctttgcg accagccgag
     2461 taagcatgtc tgctccctct cgcccgggaa caccccggcg acggtccgat gcgaccctca
     2521 ctaactgcac agtatgcctc tccgaactcg ttggttagcc gttcttttat gtcccaagga
     2581 aaatgagggg atgagtgagc ttgcggacag cctcctagca aatcctgatc tcgatcccac
     2641 cgacttgatg gcccggttcg ttgatcggcg ggataatggg aactactctt gcaccggaga
     2701 ctgcttcaac actggcaaca ccatcaacgc t
//
LOCUS       DASWQN010001187         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1832116, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001187 DASWQN010000000
VERSION     DASWQN010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832116"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VGC82_20165"
     CDS             complement(<1..664)
                     /locus_tag="VGC82_20165"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425644.1"
                     /translation="MLLAVAWRESGFATTATPGNGSTAAGMFQFNEDAWLDAIRIYGP
                     KHGLTGYSSLLKLEDGGTTAVSDRARRRVLDLRRDVRVSAAMAAETMVGERGELEDDL
                     GRPTRPVDLYFRHLLGPAGSRRFLRAIAATPSISSDQFLGRIARGNPIIFYRDGNPLS
                     IGDAYANVAAMLDERMVMYGDQVRHYHATLTTDGKRRPPRREAGPVAYRHEARFSVKA
                     HGE"
BASE COUNT          314 a          571 c          579 g          334 t
ORIGIN      
        1 cctctccgtg cgctttgacg gagaaccggg cttcgtgacg gtatgctacc ggtcccgctt
       61 cacgccttgg cggcctccgc ttgccatccg tggtcaacgt ggcgtggtag tgcctgacct
      121 gatcgccata catgaccatc cgttcgtcga gcatcgccgc cacattggcg taggcgtccc
      181 ctatcgagag cggattgccg tcgcgataga agatgatcgg gtttccgcgt gcgatccgac
      241 ccaggaactg gtcgctggag atcgacggcg tcgcggcgat ggcccggagg aagcgcctgc
      301 tgcctgcggg acccaggagg tgccgaaagt acaggtctac tggccgggtg ggacgtccca
      361 aatcatcttc gagctccccg cgttcgccga ccatcgtttc ggcggccata gcggctgaga
      421 cgcgtacgtc acggcgcaga tcaagaaccc ggcgacgtgc gcggtcgctg acggctgtgg
      481 tcccgccgtc ctcgagcttg agcaggctag agtagccggt cagcccgtgc ttagggccgt
      541 agatccgaat tgcatccagc caagcgtcct cattgaactg gaacatgcca gcggctgttg
      601 agccgttccc gggcgtggcg gtggtcgcaa agccgctttc ccgccaggcc accgccagga
      661 gcaatgaagg cgccaagcca gccgcggtcg cctgcggagc tacggcgccg atgacccacg
      721 gcggggccaa tggatcatct cgtctgatgc ccgacggggc aggtggcggt ggcaagtggt
      781 gtgccgcctt tccatgtgct gcccgccttg gatgcgtctg gaccactggt tcatgttttg
      841 ccggatgacg cgcggagttc gtcttcgcag cgctatcgtg ttcgcgtttc ccgtgcgcca
      901 gcgcatccca aggcacgacc gcggataccg ccgcaaccat ggtggccgcg cgaagccaag
      961 ctggtaggct ccgcattcgt tgcactcccc tggccacgcg tctcatcccc cggcctgtaa
     1021 cgggacgtat gcaacgcacg gtgcacgatc tcgttcggct gaactcctcc ccaacctgaa
     1081 ggaggcttcc gcattcgcca ccatcgcatg cctttggccg ccgcagcgcg ggcgcgcggt
     1141 ccagctcaca gttacgcgcg atgactgccc ggcggcttcc tcgcaggctt cacgggcaac
     1201 ggctgccggc cggcgatagt ccgtgcacaa gcacgggcgt ggtcattatc ccggatgccc
     1261 gggaacagtc ggcgcagttt caccgcagcc gacagttcgc ctccacactc gtaggcggtg
     1321 cggatcgcgg tggcgtcgga ttcggtaacg acgaacatac tggaccatga acccacggcc
     1381 atgggcgagc aagctgaacg gaccagggcg caggatggcc tcttggaaaa gggccggcat
     1441 gatgacgtaa tcgtcgttcc acttttgaat gccgggtagt gaccaaccag acggctgaga
     1501 cgcccaaaat caggccagac gaaaatccgt ggtatctcct ggcaacgctg tatggggaac
     1561 cgacattcga cgattcagag ctgcaagcca gaaaccgtat ggcttggaac cgctgatcga
     1621 gatcatgttg ccccgatggc ttgcgcctgc gcgttggcaa tgacgttggt ctggcgacat
     1681 tgcgccgcgt tatgatagtg ctgcgcggat gatcgcgcca ccgccggcgt gcggtgtggc
     1741 tggcggctgg cgtgaccgac atgcgcaaag gcttcgacga actggtcacc ttagtgca
//
LOCUS       DASWQN010001188         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1699531, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001188 DASWQN010000000
VERSION     DASWQN010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699531"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..875)
                     /locus_tag="VGC82_20170"
     CDS             complement(120..875)
                     /locus_tag="VGC82_20170"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0425645.1"
                     /translation="MDGLTWAFFALAAFAGGFVSGFSGFAMGLVVSGAWLHIITPLQT
                     AALIAGYGLLTQGYGIVKLRHAFNWRAILPLAVGSAVGIPVGVYLLTYINPLLLRAGV
                     GAVLLTYSSYSLLRPAIGPFAIGTGGDLAIGFANGILGGMTGLGGVISTISCQWRGWP
                     KDVQRGVFQPVLFAAFVVINVSLGFAGAFTPETFRLYAVGLPFLLAGLWGGFKLYGSI
                     DDETFRKSVLLLLLISGLSLILSASGLHGARGA"
     gene            complement(1160..1681)
                     /locus_tag="VGC82_20175"
     CDS             complement(1160..1681)
                     /locus_tag="VGC82_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY0425646.1"
                     /translation="MVLNADFRPLSYYPLSLWSWQDAIKAVFLDRVNIVEHYDRAVRS
                     PSFEIQLPSVVSLKSFVKPTTHPAFTRFNVFLRDRFACQYCSANEDLTFDHIIPRSKG
                     GQTTWDNVVAACSPCNLRKGNLTPQQARMFPRQPPFAPTVHQLHRNGRLFPPNYLHDS
                     WLDYLYWDTELDP"
BASE COUNT          474 a          628 c          670 g          342 t
ORIGIN      
        1 agcggggttt tgactgggcc gtgatcgacc cggcgttctg gcgcggctct caagccggga
       61 acggctgtct agtcctggcg agcagtttcg tctatggccg gtggcgtagc tcacctctgc
      121 tacgcgcccc tggcgccgtg caatccgctg gcagacagga tcagcgaaag cccggatatc
      181 agcaacaaca aaagtaccga cttgcgaaac gtttcatcat cgatcgatcc atagagcttg
      241 aaaccgcccc agagaccggc gagtaaaaac ggaaggccga ccgcatagag acggaacgtc
      301 tccggcgtga acgcgccggc gaaccccaga gaaacgttga taacgacaaa agctgcaaac
      361 agaaccggct ggaatacgcc gcgctgtacg tccttcggcc acccgcgcca ctgacaggag
      421 atcgtcgaga tgacgccgcc caagcctgtc attccgccga ggatgccgtt tgcgaagccg
      481 attgcgaggt ctccgccggt gccgatcgcg aagggaccga tcgcaggccg cagcagacta
      541 taggaactgt aggtcaagag gacggcgccg acgcccgcac gaagcaggag cggattaatg
      601 taggtgagca ggtagactcc gaccggaata cccactgcac taccgactgc caaaggcaag
      661 atggcccgcc agttaaacgc gtgacgcagc ttgacgatgc cgtagccttg ggtaagcagg
      721 ccgtagcctg caatcaaggc ggcggtctgg agaggcgtga tgatgtgaag ccacgcgccg
      781 gatacgacga ggcccatcgc gaacccggaa aacccgctga caaagcctcc ggcaaaggcc
      841 gccaacgcaa aaaacgccca ggttagtcca tccatgatac gccctcccga aagaacggcg
      901 agcgcttgga tggaaaccca tctgccgggg aggctctgat gatccccggc ggtcagctta
      961 cctagggcat caacaggaga caaccaccat ctcaaaacct ggatgggagc taacatgatg
     1021 cgagctaaca tagagtccgg ctcggggatg ggcggactcc ccgtcagact tttccgtcca
     1081 agctctgcgc gacacacgat ccgtcgtgga gaacgcgcat ggacaccgaa cgacgagcgt
     1141 cgcacgaccg atcgcggcgt tacggatcga gctcggtatc ccagtataaa tagtccagcc
     1201 agctgtcatg cagatagttc ggcgggaaca gccggccgtt gcgatgcagc tggtgcaccg
     1261 tcggtgcgaa cggcggctgc cgcggaaaca tccgcgcctg ttgcggcgtc aggttcccct
     1321 tgcgcagatt gcagggcgaa caggccgcca ccacgttatc ccaggtggtc tggccgccct
     1381 tgctgcgcgg gatgatgtga tcgaaggtga ggtcctcgtt ggcggagcag tactggcagg
     1441 cgaagcggtc gcgcaggaac acgttgaacc gggtgaaggc cggatgcgtg gtcggtttga
     1501 cgaaggactt cagcgatacc acgctcggca gctgaatctc gaacgaagga ctgcgcaccg
     1561 cgcggtcgta atgttcgacg atgttgacgc ggtcgaggaa caccgccttg atcgcgtcct
     1621 gccacgacca gagagacagc ggatagtaac tcagcggccg aaaatcggca ttcagcacca
     1681 gaaccggcca accaccttgc gagacatgtg cgttcaagta acgctcctgg cccccatcta
     1741 agctgcgaag cagcgtgagt tgacatacta catgcagcgt gccggcgttg tgaagagcgt
     1801 tggcggcctt atccgctatc cctgccgcca ctttgcggcg ggattgcgcc agtcgcgtga
     1861 acatcgttaa acggcactga gtggcggctg tatgccgcct gcggacgcca gcggaatggc
     1921 tacggaatcc agatttgccc atgcgatgcg gcgttatgcg gaggaagccg aacagcgtcg
     1981 gcgcgatgcg atcggagaga tttgacccgc gccgcggacg ggcacagggt aacgaaagct
     2041 caatcggcga ggggaccatg cctaggctgc cgaccatgat cgctgcgatc ctgctgggcc
     2101 tgggatgggc cggc
//
LOCUS       DASWQN010001189         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2359092, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001189 DASWQN010000000
VERSION     DASWQN010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2359092"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VGC82_20180"
     CDS             complement(<1..191)
                     /locus_tag="VGC82_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425647.1"
                     /translation="MPQLSSLPDQNPDHWSVAGQTRPCHLLNQISQANHFCFVYAVSG
                     HPHAIIRETNVQPRTFYRG"
     gene            322..1227
                     /locus_tag="VGC82_20185"
     CDS             322..1227
                     /locus_tag="VGC82_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425648.1"
                     /translation="MPTGETNPILTDCPVQAPSLLASISPASGLPDPAVHPPSHQSRT
                     SATGWLSRLKRRFAPVHQGRIVDIGWTLRQTKASLIWEPPRPVLNKGPRSGHSKVVSV
                     CPAMLDHESRLIEVTCPVDARIRFRRDAQGRPTLENALGDASPIRSGHLAQMVKLLSE
                     KEWRHPQRPIVQVITPYLFLADEPVWLTQLPPVHAYLPTPWPGLLISGRFPIDVWPRT
                     LMWAFEWHDTTKDLILKRGEPWFTVRLETMDPSRKTRLIRAKLTPELEEYLQGTDGVS
                     NYIRGTFSLFDTARRRRPKILLMAE"
     gene            1315..>2208
                     /locus_tag="VGC82_20190"
     CDS             1315..>2208
                     /locus_tag="VGC82_20190"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY0425649.1"
                     /translation="MTEILHADGAGVTRAASLLRDGKLVAFGTETVYGLGADATNAEA
                     VAAIFQAKMRPRFNPLICHYSCAAAAFRHVVADERAWRAASTFWPGPLTLVLPRQPDC
                     PVALLTGAGLDTLAVRVPNHPVAMSLLRHAGRPIAAPSANRSGQVSPTSARHVLDGLR
                     GRIQAVLDSGPCNVGIESTVLDLSGPEPRLLRPGGVTTEALEACLGPIQREIAAAGAM
                     HDFRSPGLMVSHYAPELLVRLAATAVAGDEALLAFGPPLTGALCCFQLSDESDTVEAA
                     ARLFEGLRWLDAEGIRLGARGI"
BASE COUNT          379 a          682 c          724 g          423 t
ORIGIN      
        1 tcgccgcggt aaaaggtcct gggttgcacg ttcgtttcac ggatgatcgc gtggggatgg
       61 ccgctgacag catacacaaa gcagaaatga ttggcctgcg aaatttggtt gagcaggtgg
      121 cacggccggg tttggccggc cacggaccag tggtctggat tttgatcggg caagctggaa
      181 agctgaggca tcgtatcatt atttcgttgc gtttgcggcg acaacctgcg acaaacctgc
      241 ggctttgcgc tcgctaccat taaacgcgta acgaaatcgc ccgcagggcg gaaaggccgt
      301 aaattccgag gctctcatcg aatgcctaca ggcgaaacga acccaatttt gacggattgt
      361 ccggttcagg cgccaagcct gcttgcgtcg atatcgccgg catccggcct acccgacccg
      421 gcggtccatc cgccatcgca ccaaagccgt acgtctgcaa caggttggct gtcgcgactc
      481 aaacgccggt tcgcgccggt ccaccaaggc cgcatcgtcg atatcggctg gacgctgcgg
      541 cagacgaagg ccagtttgat ctgggagccg ccgcgaccgg tgttgaacaa gggaccgcgg
      601 tccggccact cgaaggtcgt cagcgtctgc ccggcgatgc tcgaccacga gtcccgactg
      661 atcgaggtca cctgtccagt cgatgcgcgt atccgcttcc gccgcgatgc ccagggccgc
      721 ccaacgctgg agaacgcgct tggggacgcg tctccgatcc gctctggaca tctggcgcaa
      781 atggtcaagc tattaagcga aaaggagtgg cgtcacccgc aacgaccgat cgtccaggtc
      841 atcaccccct atctgttctt ggcggacgaa cccgtatggc tgacccagct tccaccggtg
      901 catgcttacc tgcccacgcc gtggcccggc ctgctgatca gcggacggtt tcccatcgac
      961 gtctggccac gcacgctgat gtgggcattc gagtggcacg acacgaccaa ggacctgatc
     1021 ctgaagcgcg gtgagccatg gttcacggtg cgattggaaa ccatggatcc cagtcgcaag
     1081 acacggctga tcagggcaaa gctgacgccg gagctggagg aataccttca gggcaccgat
     1141 ggcgtttcga actacattcg gggcaccttc agccttttcg ataccgcccg ccgccgccgg
     1201 ccaaagatcc tgctgatggc ggaatagagc tcgccacctg agggccccag gcacctcgcc
     1261 cgggatgtgg cgccgctttg caatatcggc ctgcagtggg tattggctgc tgccatgact
     1321 gaaatcttgc atgcggacgg tgccggcgtc acgcgggcag cttcgttgct gcgcgatggc
     1381 aagctggtcg cgttcggcac ggaaacggtc tatggcctgg gcgcggatgc gaccaacgcc
     1441 gaggcggtcg ccgcaatttt ccaggccaag atgcggccgc ggttcaatcc gttgatctgt
     1501 cattattcgt gcgccgcggc tgcgttccga catgtcgttg ccgacgaacg ggcttggcgc
     1561 gcggcgagca cgttctggcc gggcccactg acgctggtgc tgccgcgaca gccggattgt
     1621 ccggttgcct tgctgaccgg ggctgggctc gacaccttgg cggtccgcgt accgaaccat
     1681 ccggtggcga tgtcgctgct gcgccatgcg ggtcggccga tcgcggcgcc ttccgctaac
     1741 cggtcagggc aagtcagccc aacctctgcg cggcatgtgc tggacggtct tcgtggacgt
     1801 atccaggccg tgctggacag cgggccgtgc aacgtcggga tcgagtccac cgtgttggac
     1861 ctgagcggac ccgaacccag gctgcttcgg cccggtggcg tgacgacgga agctcttgaa
     1921 gcgtgcctcg gcccgattca gcgtgagatc gcggcggctg gcgcgatgca tgattttcgc
     1981 tctcccggcc tgatggtttc gcactatgcg ccggaacttc tcgtcagact cgcggccacg
     2041 gcggtggcgg gtgacgaggc gctgctggct ttcgggccgc cgctgacggg cgcgctgtgt
     2101 tgtttccaac tgagtgacga aagcgacacg gtcgaggctg ctgcgcgcct gttcgaagga
     2161 ctgcgctggt tggatgcgga gggtatccgg ctgggtgcgc ggggcatc
//
LOCUS       DASWQN010001190         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2624719, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001190 DASWQN010000000
VERSION     DASWQN010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2624719"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VGC82_20195"
     CDS             <1..980
                     /locus_tag="VGC82_20195"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEY0425650.1"
                     /translation="PQFPPTLRRVEFLAFPNVQLLDVSGPLQVLATANRQAEGAGHPP
                     PYALKVVSSMPGLNMSSAGLALLAQALPPADDPLDTLIVAGGIGVHRVCLDQALVGWV
                     QRRAQAARRVASVCSGAFLLATAGLLDGRRATTHWSRCADLAAAFPAIRVEQDPIYVT
                     DGKVWTSAGVTAGIDMALALLEADLGRPMATAVARHLVVFAKRPGGQAQFSSGLALAG
                     SDETFDGLHDWMKQNLRSNLGVPAMAERAGMSERSFIRHYRRIIGLTPARAVERLRVE
                     AARQALADNSLPVKRIARDCGFGSEETMRRSFMRVLAVPPQAYRERFPA"
     gene            complement(989..3175)
                     /gene="uvrB"
                     /locus_tag="VGC82_20200"
     CDS             complement(989..3175)
                     /gene="uvrB"
                     /locus_tag="VGC82_20200"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY0425651.1"
                     /translation="MSQTLVRPPPALFMPQKQAAKPLTRRLKVVSEYEPAGDQPTAIT
                     ELVRGVDAEERDQVLLGVTGSGKTFTMAKVIEAVQKPTLILAPNKTLAAQLYGEMKSF
                     FPDNAVEYFVSYYDYYQPEAYVPRTDTYIEKDSQINEQIDRMRHAATQSLLERNDVVV
                     VASVSCIYGIGSVESYARMVAKLQVGGRIDRDTLAKALVDLQYRRNDTAFQRGSFRLR
                     GEVVDIFPSHYEDRAWRVTLFGDEIEAVHEFDPLTGDKTADLKEITVYANSHYVTPRP
                     TLTQAIRDIKVELKIRLDELHAQGKLLEAQRLEQRTTFDIEMIETTGSCKSIENYSRY
                     LTGRKPGEPPPTLFEYLPENALLIVDESHVTVPQIGGMFKGDFNRKTVLSEFGFRLPS
                     CTDNRPLKFEEWERFRPLTVFVSATPGPWEMERTAGVFAEQVIRPTGLIDPVTEVRPV
                     EHQVDDLLHECKEVASRGGRVLVTTLTKRMAEELTDYLTEQGVKVRYLHSDIDTLERI
                     EIIRDLRLGVFDVLVGINLLREGLDIPECALVAILDADKEGFLRSRTSLIQTIGRAAR
                     NIDGKVVLYADVMTRSLRASIEETERRRNKQRSWNEANGITPMSIRKQIGKVLESVFE
                     QDYVTVAPIKDSDTTEFVGKNLKASIADLEKRMRAAAANLEFEQAARFRDEIKRLEAM
                     ELGLPPPPVASALSRPKRDRTPEPLGPGGGGYDPAKRRGGAKRRGP"
BASE COUNT          538 a         1077 c          994 g          632 t
ORIGIN      
        1 cccctcaatt tccgccaacg cttcgcaggg ttgaattcct cgctttcccg aacgtgcagc
       61 ttctggacgt gagcggcccg ttgcaggtcc tggccaccgc caaccgtcag gctgagggtg
      121 ccggtcatcc cccgccctac gcactgaagg tggtatcttc tatgccgggg ttgaacatgt
      181 cctctgccgg attggcgttg ctggcgcagg cgttgccgcc ggccgatgat ccgctcgaca
      241 ccttgatcgt tgccggcggc attggtgtgc accgcgtatg tttggatcag gctttggtcg
      301 gctgggtgca gcgtcgcgca caggctgcgc ggcgtgtcgc gtccgtctgt tcgggcgcat
      361 tccttcttgc gacggccggg ttgttggacg gccggcgagc cacgacgcac tggtcccgct
      421 gcgccgacct cgccgcggcg ttccctgcga tccgggtgga gcaggatccg atctacgtca
      481 cagatggcaa ggtctggacc tcagccggcg tgacagcggg tatcgacatg gcgctggccc
      541 tgctggaggc ggaccttggc cggccaatgg caacggccgt cgcccgccac ctggtggttt
      601 tcgcaaaacg ccccggcgga caggcgcaat tcagctccgg actggcgctt gcgggttcgg
      661 atgaaacgtt cgacggactg cacgactgga tgaaacagaa cctgaggagc aacctgggag
      721 tgcctgcgat ggccgaacgg gctgggatga gcgaacgcag tttcattcgg cactatcgcc
      781 gcataatcgg attgacaccg gccagagcgg tcgaacggct gcgggtcgaa gccgcgcgcc
      841 aggcgctcgc ggacaacagc ctgccggtga aacgcatcgc ccgcgattgc ggctttggct
      901 ccgaagaaac gatgcggcgc agcttcatgc gggtgttggc agtaccgcca caggcctacc
      961 gggaacgctt tccggcgtaa ttgccatctt acggcccgcg ccgtttcgcc ccaccgcgtc
     1021 gcttcgccgg atcgtagccg ccgccacccg gtccaagcgg ctccggggtc cggtcgcgtt
     1081 tcgggcgcga caatgcgctg gcaaccggtg gcggaggcag ccccagttcc atcgcttcca
     1141 gccgcttgat ctcgtcgcga aaccgcgccg cctgttcgaa ctccagattg gcggcggcag
     1201 cacgcatccg cttctccagg tcggcaatcg aggccttcag gttcttgccg acaaactccg
     1261 tggtgtcgga atcctttatc ggcgcgaccg tgacatagtc ctgctcgaac acgctttcca
     1321 gcaccttgcc gatctgcttg cggatcgaca tcggcgtgat gccgttcgct tcgttccagc
     1381 tcctctgctt attgcgtcgc cgctcggttt cctcgatcga cgcgcgcagc gaccgcgtca
     1441 taacatcggc gtacagcacg accttgccgt cgatattgcg cgccgcccgg ccgatcgtct
     1501 ggatcagcga ggttcgcgag cgcaggaagc cttccttgtc cgcatccaga atcgccacca
     1561 gcgcgcattc cggaatatcg agaccctcgc gcaacaggtt gatcccgacc agcacgtcga
     1621 acacgcccaa acgaagatcg cggatgatct cgatgcgctc cagcgtgtcg atgtcggaat
     1681 gcagatagcg caccttcacg ccctgttccg tcaggtagtc ggtcagttcc tccgccatcc
     1741 gcttggtcag tgtcgtgacc aggacacgcc caccccgcga ggccacctcc ttgcactcgt
     1801 gcagcaggtc atcgacctga tgctcgaccg gtcgaacctc cgtaaccgga tcgatcaaac
     1861 ccgttggacg gatcacctgt tcggcgaata ccccggccgt gcgctccatt tcccaaggac
     1921 ccggcgtcgc cgacacgaac accgtcagcg gccggaaccg ctcccattcc tcgaacttca
     1981 gcggccggtt gtcggtgcag gacggcagcc gaaagccaaa ctccgacagc accgtcttgc
     2041 ggttgaagtc gcctttgaac atgccgccga tctgcggcac cgtgacgtgg ctttcatcga
     2101 cgatcagcag cgcgttctcc ggcaaatact cgaacagcgt cggcggcggc tcgcccggct
     2161 tgcgtcccgt caggtaccgc gagtagttct cgatcgactt gcacgaaccc gtcgtctcga
     2221 tcatctcgat gtcgaaggtc gtccgctgct ccagccgctg cgcctccagc agcttgccct
     2281 gggcgtgcaa ctcgtccagc cggatcttca gctccacctt gatgtcgcgg atcgcctggg
     2341 tcagcgtcgg ccgaggcgtc acgtagtggc tgttggcgta aaccgtgatc tccttcaggt
     2401 cggcggtctt gtcgccggtt agcggatcga actcgtgcac cgcctcaatc tcatccccga
     2461 acagcgttac ccgccatgcc cggtcttcgt aatgacttgg gaagatatcg acgacctcgc
     2521 cgcgcagccg gaacgagccg cgctggaacg cggtgtcgtt acgccggtat tgcagatcga
     2581 ccagcgcctt ggccagggtg tcgcggtcga tccgcccacc gacctgcagc ttcgccacca
     2641 tcctggcata gctctcgacc gacccgatac cgtagatgca ggacaccgag gccaccacga
     2701 cgacgtcgtt ccgttccagc agggactgcg tggcggcatg acgcatgcgg tcgatctgct
     2761 cgttgatctg tgagtctttc tcgatatacg tgtcggtgcg cggaacatag gcctccggct
     2821 ggtaatagtc gtagtagctg acaaagtatt cgaccgcatt gtccgggaag aagctcttca
     2881 tctcgccgta aagctgggcc gcgagggtct tgttcggcgc caggatcagg gtcggcttct
     2941 gcaccgcctc gatcaccttg gccatggtga aggtcttgcc tgacccggtg acaccgagca
     3001 gaacctgatc gcgctcctcg gcatcgacgc cgcggacgag ttccgtgatc gcagtcggct
     3061 ggtcgccggc gggttcgtat tcggacacaa ccttcagtcg ccttgtcagc ggcttggcgg
     3121 cctgcttctg cggcatgaac aaggcgggcg gcgggcggac cagggtctgc gacatcaggg
     3181 gctcctcaga cgtgaaagat ggagcgccgc ccccccgacg tcgagggggc acgttgacga
     3241 a
//
LOCUS       DASWQN010001191         3031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_908641, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001191 DASWQN010000000
VERSION     DASWQN010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908641"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1115)
                     /locus_tag="VGC82_20205"
     CDS             complement(126..1115)
                     /locus_tag="VGC82_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425652.1"
                     /translation="MTPMTIEVRNLRTHFATRAGIVKAVDDVSFAVGRGQVLGIVGES
                     GSGKTVTGFSIIGLVDPPGRITGGQILYQGQDLAKLTEPQMRALRGNRIAMIFQDPMM
                     TLNPVLRIDTQMIEAVQAHTKLVRAEARTKARDALGMVGIPSPEERLSAYPHQFSGGM
                     RQRVAIAIALLHNPDLLIADEPTTALDVTIQAQILSEVQRLAVEHGTALIWITHDLSV
                     IAGLADRIAVMYAGRIVESGEAADVLDRPFHPYTAGLIGSVPSRNRRGQPLRQIPGMT
                     PSLLSLPAGCAFRSRCSRADATCEQQPPVAGAGHTARCFHPLAGFHPLPEAAA"
     gene            complement(1112..2005)
                     /locus_tag="VGC82_20210"
     CDS             complement(1112..2005)
                     /locus_tag="VGC82_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0425653.1"
                     /translation="MTIATIAEETPLHRFAAEFAQSKLAVAGLIVFILIAAAAILAPL
                     IAPQNPYDLAQLDIMDGRLPPGSASAAGYTYLLGTDDQGRDMFSGMLYGLRISLAVGV
                     GSALFAAVVGTTLGLLAAFYGRRTDAVIMRFVDLQLSFPSILVALMILAILGKGVMNV
                     VLALVIVEWAYYARTIRATAMVERRREYIEAALCLALPWRRIMFRHLLPNCLPPLIVI
                     GTQQVARAIALEATLSFLGLGVPITEPSLGLLIANGYQYMLSGKYWISFYPGIALLVT
                     IVSINLVGDQLRDVLNPRLSR"
     gene            2322..2561
                     /locus_tag="VGC82_20215"
     CDS             2322..2561
                     /locus_tag="VGC82_20215"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY0425654.1"
                     /translation="MDVIGAFEAKTHLSSLLERVAKGENFTITKHGKPVAHLVPVDQR
                     NPEQTRAVVQRMEELAAGLTLDGDWKEFRDAGRKW"
     gene            2555..2974
                     /locus_tag="VGC82_20220"
     CDS             2555..2974
                     /locus_tag="VGC82_20220"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0425655.1"
                     /translation="MVDSAVVIDASVTAAWCFHDEATEASRTLLRSLPHRQVVVPLLW
                     HAECANLLLTSERRKRVSAEQCAELLELLGGLPIETDHETARIPGPVFRLARTHGLTV
                     YDAIYANLASRRGMTLATRDKALRRAAAAMRVAVIET"
BASE COUNT          575 a          851 c         1065 g          540 t
ORIGIN      
        1 cctgaccacc tggttcggcc gcggtctgcc aattcgcgcg gccagtgctc gggttacacg
       61 ggcggcggca tcgtggcttg gaccgaagta tttggaaacg ccgcgcagct ccatgatcgg
      121 cttgttcatg cggcggcttc cggcaacgga tgaaagcccg ccaacggatg aaagcagcgt
      181 gcggtatgtc cggccccggc caccggaggc tgttgttcgc aggtggcgtc cgcccgagaa
      241 cagcgtgacc gaaaggcgca gccggccggc agggacagca ggctgggggt catcccaggg
      301 atttgccgca atggctggcc acgacgattt cggctgggaa cgctgccgat caggccggcc
      361 gtgtatgggt gaaacggacg gtcgagtacg tccgccgcct caccggattc gacgatgcgg
      421 ccggcgtaca tgacggcgat gcggtccgcc aagccggcga tgaccgacag gtcgtgggtg
      481 atccagatca gggcggtacc gtgttcgacg gccagacgct gcacttcaga caggatttgg
      541 gcctggatcg tcacatccag cgcggttgtc ggctcgtctg cgatcagcag gtcgggattg
      601 tgcagcagtg cgatggcgat ggcgacgcgc tggcgcatgc cgccggagaa ctggtgcgga
      661 taggcgctca agcgttcctc cgggctggga atgccgacca tgccaagggc gtcgcgggct
      721 ttggtgcgcg cttcggcccg aacgagcttg gtgtgcgcct ggactgcctc gatcatctgg
      781 gtgtcgatgc gcaggaccgg attcagggtc atcatcgggt cctggaaaat catcgcgatg
      841 cggttgccgc gcagcgcccg catctgtggt tccgtcagct tcgccagatc ttgcccctga
      901 tacaggatct ggccgccggt tatgcggccg ggcggatcga ccaggccgat gatggaaaag
      961 ccggtcacgg tcttgccgga gccggattcg ccgacgatgc ccagcacttg cccgcggccg
     1021 acggcgaacg agacgtcgtc caccgccttg acgatgccgg cgcgcgtggc gaaatgggtg
     1081 cgcaggttgc ggacctcgat tgtcatcggg gtcatctcga cagccttggg ttcaacacgt
     1141 cgcgaagctg gtcgccgaca agattgatgg agacgatggt gaccagcagg gcgatgccgg
     1201 ggtagaagct gatccaatac ttgccggaga gcatgtactg gtagccgttg gcgatgagta
     1261 aacccagcga cggctctgta atcggaaccc ccagacccag aaaagacaaa gtcgcttcca
     1321 gcgcaattgc tcgggctacc tgttgagtgc caataacgat cagcggcggc aggcagttcg
     1381 gcagcagatg gcggaacatg atgcggcgcc agggcagggc gaggcacaac gctgcctcga
     1441 tatactcccg ccggcgttcc accatcgccg tcgcccggat ggtgcgggcg tagtaggccc
     1501 actcgacgat gaccaaagcg aggacgacgt tcatgacccc tttgccgagg atggcgagga
     1561 tcatcagcgc gaccaggatg gaggggaagg aaagctgcaa atccacgaac cgcatgatga
     1621 ccgcgtcggt gcggcggccg tagaaagcgg cgagcagccc gagggtggtg ccgacgacgg
     1681 cggcgaacaa ggcggaaccg acgccgaccg cgaggctgat ccgcaagccg tagagcatgc
     1741 cggagaacat gtcccggccc tggtcgtcgg taccgagcag gtaggtgtag ccggcggctg
     1801 aggcggatcc gggcggcagg cgtccgtcca tgatgtcaag ctgcgcgagg tcgtatgggt
     1861 tctgcggcgc gatcaacggt gccaggatcg cggcggcggc gatcaggatg aatacgatca
     1921 ggccggcgac agcgagcttg gattgcgcga actcggcggc gaagcgatgg aggggggtct
     1981 cctcggcgat ggtggcgatg gtcatcgggt tcggttgggg ctttctctcg ttgggcggct
     2041 atggatcaaa ggtcgatgat gtatgattgg ggacgggtac tatagagcgt gatcacgctg
     2101 ggcggaacgt ccgtcgtccg aagaacgcga tgttggcgct ccgtcgtccg aagaacgcgc
     2161 tgttggcgcg atgcgtttga atcacgcgat caaacaagag gatagactat tgggggccgg
     2221 cacagtcagg ctgaaactgg tctcgccctg agattctctt gaacgcaact ggccagcgat
     2281 gtatatggcg actgacgaag actggttgga gactggttga tatggatgtc atcggcgcct
     2341 tcgaggctaa gacccatctt tcgtccttgc ttgaacgcgt ggcgaaaggc gaaaacttta
     2401 cgattacgaa gcacgggaag ccggtggccc atcttgttcc ggtggatcag cgaaatcctg
     2461 agcagaccag ggccgttgtc caacggatgg aggaactcgc cgccggtctg acattggatg
     2521 gcgactggaa ggaatttcgc gacgccgggc gtaaatggta gactccgcgg ttgtcatcga
     2581 cgcatccgta accgccgcct ggtgcttcca cgatgaagca acggaggcca gccggacgct
     2641 gctccgcagc ttgccgcaca ggcaggtggt ggtgcccctg ctctggcacg ccgagtgcgc
     2701 caacctgctg ctgacgagcg aacggcgcaa acgtgtctcc gccgaacaat gcgccgaatt
     2761 gctcgaattg ctcggtggtc tgccgatcga gacggatcat gagacagcgc gcattcccgg
     2821 gccggtgttc cggctggcga ggacccatgg gttgacggtt tatgacgcga tctatgccaa
     2881 tcttgcgtct cggcggggca tgactttggc gacgcgtgac aaggctttga ggcgggctgc
     2941 ggcggcgatg cgggtggcgg tgatcgagac atgacctccc tcgcctggga gatcagcggc
     3001 ttacaagaaa ggtggagccc agtctgtgct c
//
LOCUS       DASWQN010001192         5681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_512075, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001192 DASWQN010000000
VERSION     DASWQN010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512075"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..2981)
                     /gene="gcvP"
                     /locus_tag="VGC82_20225"
     CDS             complement(105..2981)
                     /gene="gcvP"
                     /locus_tag="VGC82_20225"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEY0425656.1"
                     /translation="MDAGAELAELEENDGFVRRHIGPSETELTAMLHAVGSATLDDVA
                     GKTIPASIRSNDTISLPPPIDEAATIAELRALAAQNAPIKSLIGMGYHGTVTPPVILR
                     NVLENPGWYTAYTPYQAEIAQGRLEALLNFQTMICELTGMQIANASLLDEATAAAEAM
                     AMAHALSKSKSDILAVSTDLHPQTLAVLETRAKPLGIALVPVAPGDLAAIGAANPFAL
                     LLQYPGSTGALRDMSEEIATAHEVDALAIVATDPLALALLTPPGEMGADIVVGSAQRF
                     GVPMGFGGPHAGFFATRDAYKRAMPGRLVGVSLDAAGQPAMRLALQTREQHIRREKAT
                     SNICTAQVLLAVIAGFYAVWHGPEGLYRIARRVSLQARMLADAALRAGLRLRHDSFFD
                     TIVVDAGHSAQASELLVRAAAAGYNLRRIDDTCVGIALDETVTRADLTALADLLGADL
                     GGAVASIPAPVLRGSAFLQQAVFNQHHAEHEMLRYLKRLEDKDIALNRSMIPLGSCTM
                     KLNATAEMIAITLPGFADIHPFAPADQTIGYLTLIRRLETWLCAATGFAEVSLQPNAG
                     SQGEYAGLLVIRAWHESRGERHRDVCLIPSSAHGTNPASAAMAGLKVVVVGCDRDGNV
                     DLADLRAKAEKHAARLAALMITYPSTHGVFEAGIRDICAVVHEHGGQVYMDGANMNAQ
                     VGLTSPASIGADVCHLNLHKTFCIPHGGGGPGVGPIGVAAHLAPFLPNHPLRPDAGPA
                     TGIGPVSAAPFGSALILPISYAYIRMMGAAGLKRASEVAILSANYIAHRLRDAYPILY
                     AGPGGMVAHECIIDCRGFANEAGIQVEDIAKRLQDYGYHAPTMSWPVAGTLMIEPTES
                     ESKAELDRFCDAMIAIRAEIAAIATGKLDRTDNPLKHAPHTAGEVTADVWAHAYTRTQ
                     AAFPLPWVAAAKYWPPVKRVDNVYGDRNLACTCAPLEAYAQAAE"
     gene            complement(3002..3388)
                     /gene="gcvH"
                     /locus_tag="VGC82_20230"
     CDS             complement(3002..3388)
                     /gene="gcvH"
                     /locus_tag="VGC82_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY0425657.1"
                     /translation="MANKSADKRFTKDHEWVLLDGDIATIGITDHAQEQLGDLVHVEL
                     PELDRQVTEAETCAVVESVKAASDVYAPLAGKVVEINETILDDPSIVNSDAEGEGWFF
                     RLELDDADAFETLMDQDAYDEFLETL"
     gene            complement(3444..4607)
                     /gene="gcvT"
                     /locus_tag="VGC82_20235"
     CDS             complement(3444..4607)
                     /gene="gcvT"
                     /locus_tag="VGC82_20235"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY0425658.1"
                     /translation="MAQNAVPDDPTTIALHATPLDGWHRRHGARMVPFAGYAMPVQYD
                     LAGELAARCKGGVLAEHLHTRSRAGLFDVSHMGQAMLTGSSVALALEKLVPGDIGGLK
                     PGRQRYTLLTNEAGGIIDDLMVARLDEDRLFLVVNSSRKDIDFTHLAANLPDHIALTP
                     QEDRALLALQGPAAVTVMGRLAPQAAALPFMGVAAVTIEGIECLISRSGYTGEDGFEI
                     SLPGEQAETLADKLIQQPEVVPIGLGARDSLRLEAGLCLYGNDLDELTSPIEAGLTWV
                     IGKRRKMNWDFPGGLVMRDQLDNGPIRRRVGIRPDGRAPARAQTTIVADDGTEAGIVT
                     SGSFGPSVNGPVAMGYVRRDLADDGCRLNLIVRGRTIPATVVPLPFTPHRYIR"
     regulatory      complement(4758..4855)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(4975..>5681)
                     /locus_tag="VGC82_20240"
     CDS             complement(4975..>5681)
                     /locus_tag="VGC82_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425659.1"
                     /translation="VLTADGVVSLIGCLMGNPFINAVYIGHPGWKAMGGRIGYSAMTG
                     IIVLVLTWFGIIAVISALIPVVAILPILLYIGMLIGSQAFQESPHRHAPAIVLALLPQ
                     IAEWGKTQIDNALGAAGTNAAAVGTGKLAQVGVLYDGLATLGGGATLAGIMLGAVTVF
                     IIDRAFDKAAAFALAGSVLTFFGFIHGADGIGIGQSPSVAVSYLAVAGILFYCSRYAE
                     FHPMAIDLHEAAAEAD"
BASE COUNT          982 a         1801 c         1893 g         1005 t
ORIGIN      
        1 ctgactgtgc aggcgaaagt ggtctatact ctttgtttag ttggctcttt gtttgattgc
       61 gtaattcaca tgcgttggaa cgttccgctc cgcgcgatcc tgctttactc tgccgcctgg
      121 gcataggcct ccagcggggc gcaagtgcag gccaggttgc ggtcgccata aacgttgtcc
      181 acccgcttga ccggcggcca gtacttggcc gcggcaaccc acggcagcgg aaaggccgcc
      241 tgcgtgcgcg tataggcatg agcccataca tccgccgtga cctcgccggc ggtgtgcggc
      301 gcgtgcttca gcggattgtc ggttcggtcc agcttgccgg tggcgatcgc cgcgatctct
      361 gcccgtatcg cgatcatcgc atcgcagaac cgatcgagtt cagccttgga ttcgctttcc
      421 gtcggctcga tcatcagcgt gccggcgacg ggccatgaca tggtcggggc gtggtagccg
      481 taatcctgca ggcgcttggc gatgtcctcg acctggatcc cggcttcatt ggcgaagccg
      541 cggcagtcga tgatgcactc gtgcgcgacc atcccaccgg ggccggcata gagaatcggg
      601 taagcgtccc gcaaccggtg ggcgatgtag ttggcggaca ggatcgcgac ctcgctggcg
      661 cgtttcaggc cggcggcacc catcatgcgg atataggcgt aggagatcgg caggatcagc
      721 gcactgccga acggcgcggc ggaaacgggg ccgatgcccg ttgcgggtcc cgcatccgga
      781 cgcagcggat ggttgggcag gaaaggtgcc agatgggcgg cgacgccgat cggcccaact
      841 ccggggccgc cgccgccgtg gggaatacag aacgtcttgt gcaggttcag atggcagaca
      901 tcggcgccga tggaggcagg cgaggtcaat cccacctgcg cattcatgtt ggcgccgtcc
      961 atataaacct ggccgccgtg ctcgtgcacc acagcgcaga tgtcgcgaat cccggcttca
     1021 aacacgccgt gcgtggacgg gtaggtgatc atcaacgccg ccagccgggc agcatgtttt
     1081 tccgccttcg cccgcaaatc cgccagatcc acgttgccat cacggtcgca gccgacaacg
     1141 accaccttca ggccggccat cgccgcgctg gcgggattgg taccatgcgc cgaggacggg
     1201 atcaggcaca cgtcgcgatg ccgttcgccg cggctttcat gccaggcgcg gatcaccagc
     1261 aggccggcat attcgccctg cgagccggcg ttcggttgca acgagacctc ggcgaaaccg
     1321 gtggcggcgc atagccaggt ttccaggcgg cggatcaggg tcaggtagcc gatcgtctga
     1381 tcggccgggg cgaacggatg gatgtcggcg aagcccggca aggtgatcgc tatcatctcg
     1441 gccgtggcat tcagcttcat cgtgcaggaa cccagcggaa tcatgctgcg gttcagcgcg
     1501 atgtccttgt cctccaggcg cttcaggtag cgcagcatct cgtgttcggc atgatgctgg
     1561 ttgaagacgg cctgttgcag gaatgccgag ccgcgcagca ccggcgccgg aatcgatgcg
     1621 accgcaccgc cgaggtcggc accgagcaag tccgccaacg ccgtgaggtc ggcgcgggtg
     1681 acagtctcgt ccagggcaat gccgacacaa gtgtcgtcga tacggcgcag gttgtagcca
     1741 gcagcagccg cgcgaacaag caactcgctg gcctgcgcgc tatggccggc atctacgacg
     1801 atcgtgtcga aaaacgaatc atgccgcagg cgaagcccgg cccgcagtgc cgcatccgcc
     1861 agcatgcggg cttgcaacga gacgcggcgg gcgatgcggt acaggccctc gggcccgtgc
     1921 cacacggcat agaagccggc gatgacggcc agcaaaacct gcgcggtgca aatgttggag
     1981 gtggccttct cgcggcggat atgctgctcc cgggtttgca gcgccagacg catggcgggc
     2041 tggccggccg catccagcga cacgccgacc aggcggccgg gcatggcacg cttgtaggca
     2101 tccctggtgg cgaaaaatcc cgcgtgcggg ccgccaaatc ccatcgggac gccgaagcgc
     2161 tgcgccgatc cgacgacgat atcggcgccc atctcgccgg gtggggtcag cagcgccaat
     2221 gccagcggat cggtggcgac aatggccagc gcatcgacct cgtgcgcagt ggcgatttcc
     2281 tcggacatgt cgcgcagggc accggtgctg ccgggatatt gcagcaacaa cgcgaacgga
     2341 ttggcggcac cgatggccgc gagatccccg ggcgccacag gcaccaacgc gatccccaac
     2401 ggcttggcgc gcgtttccaa gacggccaag gtctgcggat gcaggtcggt cgacaccgcg
     2461 aggatatcgg atttcgactt gctgagggca tgcgccatcg ccatcgcctc ggcagcggcg
     2521 gtggcttcat ccaacaggga tgcgttggcg atctgcatgc cggtcagctc gcaaatcatg
     2581 gtctgaaagt tcagcagcgc ttccaggcgg ccttgcgcga tctcggcctg atacggcgta
     2641 taggcggtgt accagccggg attctccagc acgtttcgca gaatgaccgg cggcgtgacg
     2701 gtgccgtggt agcccatccc gatcaggctt ttgatcgggg cgttctgggc cgccagggcg
     2761 cgcagttcgg cgatcgtcgc ggcctcgtcg atcggcggcg gcaggcttat ggtgtcgttg
     2821 gaacggatcg acgctgggat cgtcttgccc gccacgtcgt ccagcgtggc cgaaccgacc
     2881 gcatgcagca tcgccgtcag ttcagtctcg gaagggccga tgtggcggcg aacgaagccg
     2941 tcgttttcct cgagttccgc cagttcggcc ccggcatcca tggtcaacgg catatccata
     3001 cttacaaggt ctccaggaac tcgtcgtagg cgtcctgatc catcagcgtt tcgaatgcgt
     3061 ccgcgtcgtc cagttcgagg cggaagaacc agccctcacc ctcggcgtcg ctgttcacga
     3121 tggatggatc atcgaggatc gtctcattga tctcgacgac cttgccggcc aagggcgcgt
     3181 aaacgtcgga ggcagccttg acgctttcaa cgaccgcaca ggtttccgct tccgtcacct
     3241 ggcggtccag ttccggcagt tcgacgtgaa ccaagtcgcc gagttgttcc tgcgcatggt
     3301 cggtgatgcc gatggtggcg atgtcgccat ccagcagcac ccattcgtgg tccttggtaa
     3361 aacgcttatc cgcgctcttg ttggccatag gggcgttcct ttcagcatat gtaacggtgg
     3421 ggtcagcata tgtaacggtg gggtcagcgt atgtaacggt gaggggtgaa cggaagcggc
     3481 acgacggtcg ccgggatcgt cctgccgcga acgataaggt tcagcctgca gccgtcgtcg
     3541 gcaaggtctc ttcgcacata acccatcgcg acgggaccgt ttaccgaggg gccgaagctg
     3601 ccggaggtga cgatgccagc ctcggtcccg tcgtccgcga cgattgtggt ctgcgcgcgg
     3661 gcgggcgctc ttccgtccgg acggatgccg acgcggcggc ggatcggtcc attgtcgagc
     3721 tggtcgcgca tcacgaggcc gccggggaag tcccagttca tcttgcggcg cttgccgatg
     3781 acccaggtca ggccggcttc gatcgggctg gtgagttcgt ccaggtcgtt gccatagagg
     3841 caaagcccag cctctagccg aagcgaatcc cgggctccga ggccgatcgg gacgacctcc
     3901 ggctgctgga tcagcttgtc cgccagggtt tcggcttgct cgcccgggag ggagatttcg
     3961 aatccatcct caccggtgta gccggagcgg gaaatcaggc attcgatccc ttcgatcgtg
     4021 acggcggcga cacccatgaa cggcagagcg gcggcctgtg gcgccaagcg ccccatgacg
     4081 gtgacggcgg ccgggccttg cagggccagc aacgcccggt cttcctgtgg ggtcaaggca
     4141 atgtggtccg gcagattggc cgcgagatgg gtgaaatcga tatccttgcg gctggagttc
     4201 acgaccagga acaagcgatc ctcatcgagc cgcgcgacca tgaggtcgtc gatgatgcct
     4261 ccggcttcgt tggtcagcag ggtgtagcgt tggcgaccgg gcttaaggcc gccgatatcg
     4321 ccgggcacca gcttctccag cgcaagcgcc acactgcttc cagtcaacat cgcctgaccc
     4381 atatgcgaca catcgaacag ccccgcccgg gaacgggtat gcagatgttc ggccagcacg
     4441 ccgcctttgc agcgcgcggc gagttctccg gccagatcgt attgcaccgg catggcatag
     4501 ccggcgaaag gcaccatgcg ggcgccgtgg cggcggtgcc atccgtccag tggcgttgcg
     4561 tgcagtgcga tcgtggtggg gtcgtcggga acggcgttct gggccaacta cggtctccgc
     4621 gatagggcca tccgccggcg gaatgccggt ggagatgacc cccctctgtc acggtacctg
     4681 agagattcgg gggtgtggcc ccttactccg tcggtgcgga cgcctcgctt gcgcgcggcc
     4741 cggctttcca gagatccctg cccgtcgcgg tcctttatgc ctgagcgttt ccgggggccg
     4801 gttgcgcctt cggcggcggg ttgtgttgaa cccgcttctc tcccgcggcg ggtgcctggg
     4861 acaacgcgga caatgcgccc gttcgggggt ggctgccaag ggctttgatc agctccaaca
     4921 tggatgaggc cgaaatgccc ttggcgaact ggcgcggtga aagagcggcc tcggtcaatc
     4981 cgcctctgcc gccgcctcgt gcagatcgat cgccatcggg tggaactcgg cgtaccgcga
     5041 gcagtagaac aggattccgg cgaccgcgag gtaggacacc gcgaccgatg gggactggcc
     5101 gataccgatg ccgtcggcac catggataaa gccgaagaac gtcagcaccg aaccagccag
     5161 cgcgaacgcc gccgccttgt cgaaggctcg gtcgatgatg aacacggtca ccgcgcccag
     5221 catgatgccg gccagcgtgg cgccgccgcc cagcgtcgcc agcccgtcgt acagcacccc
     5281 gacctgcgcc agcttgcccg ttcccaccgc ggccgcgttt gtcccggccg cgccaagcgc
     5341 gttgtcgatc tgcgtctttc cccattcggc gatctgcggc agcaacgcca gcacgatcgc
     5401 cggcgcatgg cggtgcggcg attcctggaa cgcctgcgac ccgatcagca tcccgatata
     5461 cagcagtatg ggcaggatgg cgacgaccgg gatcagggcg ctgatgacag cgatgatgcc
     5521 gaaccaggtc agcaccagaa cgatgatacc tgtcatcgca gagtagccga tgcggccacc
     5581 catcgccttc cagccaggat gaccgatata gacggcgttg atgaacgggt tgcccatcag
     5641 gcagccgatc aggctgacga cgccgtcggc ggtcagcacg c
//
LOCUS       DASWQN010001193         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_381534, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001193 DASWQN010000000
VERSION     DASWQN010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381534"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2015
                     /locus_tag="VGC82_20245"
     CDS             <1..>2015
                     /locus_tag="VGC82_20245"
                     /inference="COORDINATES: protein motif:HMM:NF021665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HEY0425660.1"
                     /translation="LEIGTEARPHTRKATITLTNNIKDEDIGGMGGANDRSDRGILLM
                     GGTLNLHGDRQNTWTKLARTAEAGSNSIQVLNAAGWRVGDEIVLASTDFDPRQAERRT
                     ISAIKGNAITLNAKLDYMHFGKITFDVDERGEVGLLTRNVVLQASADAAEPPFYGGHV
                     MAMGASRMFVEGVEFQRMGQNLTLARYPIHWHLVGDAKGQYIRNASIHDTYNRCVTVH
                     GTNFLRVENNVTYNTVGHCFFLEDGAEHGNQFVHNLAIQIKCHTSKACAPTNLGANGE
                     NMGSNPLNRATYRADSMSGKDTLLPSDNTVTAYWITNPDNVFIDNVAAGSDENGFWWS
                     LPEHPQGKFEGTEISKAIWPRRTRMAEFRGNVAHSNYDGFMLDRNINLEGVFGLAGPS
                     HIAKENPADENSKSVKSLLADLTAYKNRNGGVWGRGELHVFRNLKAADNAIGFTNSTG
                     SFGADTFTSLVVDSRFVGETDNIGNPVTPEEKAYGRSLPKRLIPDFPIRAYEYYDYRH
                     DVVNTTFVNFQDNKQRGSGALSWLLFTGAGVTSENTVTSAKYINAKPVYFPKIDSRFD
                     SDNRGGGAYRTAAIHDLDGSTTGIPDSYVLLHDGENDSVATDDTCEIHPTWNASVCAG
                     DIGRIQFSIRRPGPTRGAGLALKDVAVRSAAPARAAAPARAAAPQKP"
BASE COUNT          456 a          617 c          599 g          343 t
ORIGIN      
        1 aactggaaat cggcaccgaa gccaggcccc atacacgcaa ggcgacaatc accctcacca
       61 acaacatcaa agacgaagac atcggtggaa tggggggagc gaatgaccgc agtgaccgcg
      121 ggatcctgct catgggcgga accctgaacc tgcacggtga tcggcagaac acctggacca
      181 agctggccag gaccgccgaa gcaggcagca attcgataca ggtcctgaac gccgccggct
      241 ggcgggtcgg cgacgagatt gttctcgcgt ccacggactt cgatccccgg caggccgagc
      301 ggcgcaccat ttccgccatc aagggcaatg cgatcacgct caacgcgaaa ctggactaca
      361 tgcatttcgg caaaatcacc ttcgacgtgg atgaacgcgg cgaagtgggc ttgctgacgc
      421 gcaatgtcgt tctccaggca tcagccgacg cggcggaacc gcccttctac ggcgggcacg
      481 tgatggcgat gggcgcgagc aggatgtttg tcgagggcgt cgagttccag cgaatgggcc
      541 agaacctgac gcttgcccgc tatccgatcc actggcatct ggtcggcgat gccaaggggc
      601 agtacatcag gaacgcctcg atccacgaca cctacaaccg ctgcgtgacc gtgcacggca
      661 cgaacttcct gcgcgttgag aacaacgtga cctacaacac cgtcggtcac tgcttcttcc
      721 tcgaagacgg cgcggagcac ggtaaccagt tcgtgcacaa cctggcgatc cagatcaagt
      781 gccatacctc caaggcctgc gcgccgacca atctcggcgc aaacggagag aacatgggaa
      841 gtaatccgct gaatcgcgcg acctacaggg cggacagcat gtccggcaag gacaccctgc
      901 tgccttcgga caacacggtg acggcttact ggatcaccaa tccggacaat gtcttcatcg
      961 acaacgtggc agcgggttcc gatgagaacg gcttctggtg gtcgttgccg gaacacccgc
     1021 aagggaagtt cgaaggtacg gagatcagca aggctatctg gccccgtcga accaggatgg
     1081 cggaattcag gggcaacgtg gcccactcta actacgacgg gttcatgctg gaccggaaca
     1141 tcaatctcga aggcgtcttc gggttggccg ggccctcgca cattgccaag gaaaatcccg
     1201 ctgacgaaaa cagcaagagc gtgaaatcgc tcctggcgga tctcaccgct tacaagaacc
     1261 gcaatggcgg cgtctggggc cgcggcgagt tgcacgtctt ccggaatctg aaggcggcgg
     1321 acaatgccat cgggtttacg aattcaacgg gttccttcgg cgctgacaca tttacctcac
     1381 tggtggttga ttcgcggttc gtgggcgaaa ccgacaacat cggcaacccc gtaacgccgg
     1441 aagaaaaggc ctacggccgc agcctgccaa aacgcctgat tccggatttc ccgatccgcg
     1501 cctacgagta ctacgactac cgtcatgacg tggtcaacac gacattcgtg aatttccagg
     1561 acaacaaaca gcgcggatca ggcgcgcttt cctggctgtt gtttacgggc gccggggtta
     1621 ccagcgaaaa caccgtcacg agtgcgaaat acatcaatgc caagcccgtg tatttcccga
     1681 agatcgactc caggttcgac agtgacaacc ggggtggtgg ggcctaccgg accgcggcga
     1741 tccacgatct ggacggctcg acgaccggca tccctgattc ctatgttctg ctccacgacg
     1801 gcgagaacga cagcgtcgcc acggacgaca cctgcgagat tcatcccacc tggaacgcct
     1861 ctgtgtgcgc gggcgatatc ggacgcattc agttcagcat caggaggccc ggacccacaa
     1921 ggggtgctgg tcttgcgctg aaagatgttg ctgtgcggtc agctgcgcct gcgcgggcgg
     1981 ctgctcctgc gcgggcggct gctcctcaaa agcca
//
LOCUS       DASWQN010001194         3848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2359682, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001194 DASWQN010000000
VERSION     DASWQN010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2359682"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(335..2629)
                     /locus_tag="VGC82_20250"
     CDS             complement(335..2629)
                     /locus_tag="VGC82_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEY0425661.1"
                     /translation="MGKHPTFQPYTAPAGGWGSARSLGNIMRREGVLASGPLVLLRHN
                     KADGYQCTSCAWIKPADPLPFEYCENGAKATAWDITSHRCTPDFFAKHTVTELMDWDD
                     YSLEQAGRLTHPLRWDAATDKYLPVSWADAFAEIGRELRAVPDPKQTVFYSSGRMSNE
                     TSYLYGLFARLYGNNNLPDSSNMCHETTSVALPESIGVPVGTVSLDDFAHAECILFFG
                     QNPGSNSPRMLHPLQEASKRGVPIITFNPLPERGLERFTNPQSPVEMLTGSSTRISSS
                     YHQVKTGGDLAALAGICKAVVELDTAAEASGQQRVIDHAFITEHTDGFASFAKWLAQQ
                     DWDELERRSGLRRAALEATAMTYCRSNATIAIYGMGLTQHQAGVETIQMLVNLQLLRG
                     NIGKQGAGICPVRGHSNVQGQRTVGITEKPELVPLDRLAAQYGFEPPREKGMDTVEAC
                     EGILHGKVRAFVMFGGNFIRAVPDRGLMEPAWRKLRLTVNVQTKLNRSHLVHGEISYI
                     LPVKSRIEVDKQGGEPQALSMEDTSACIHGSRGVRAPASKFLLSEVEVLARMAKATLD
                     PNPKVPWDAWMSDYSRIRLSIAETYPDIFHDYERRMWEPGGFHRYLPARERVWHTKTG
                     KANIITPTALEEDLDMPEIGHDVLRLMTLRSNDQFNTTVYGYSDRFRGVEGTRMIVFM
                     NRNDIARLGLREGENVKLTTSTDDGVHRELMGLRVTPYDIPVGCIGAYYPEANVLLPL
                     WHHAKGSKTPAAKNIPVMVHRQEA"
     gene            2768..2998
                     /locus_tag="VGC82_20255"
     CDS             2768..2998
                     /locus_tag="VGC82_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425662.1"
                     /translation="MVDRNYLLRKPPPPPPWKQFLDLTVLPAMIDAAGAVEGLLERVA
                     VQGRQRPALTTGMALGLACGLALLVGPRKSRN"
     gene            complement(3117..>3848)
                     /locus_tag="VGC82_20260"
     CDS             complement(3117..>3848)
                     /locus_tag="VGC82_20260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY0425663.1"
                     /translation="IRFGRVTAPRNQTGSGGHSLSSSEAPPGDLPASLRHGDIGSACV
                     HLCCDMQRLFAENTDWHTPWMSRVLPLARRIAEAHPERTIFTRFIPAFRAGEGSGTWK
                     RYYERWASMTIERLGREMVELVPELAALVPPAQVVDKRVYSPWVDSGLQSRIEALGAD
                     TIIVSGSETDVCVLATVLGAVDRGYRVIVANDAICSSADETHDASLQLYNGRYGQQVE
                     TASTDEILANWKFERDHAGRNVRSA"
BASE COUNT          695 a         1186 c         1195 g          772 t
ORIGIN      
        1 ctctccgtgg tggccgactg cccggggtag ccaggacgca agatgaagat gtggatggcc
       61 gtgctcggcg cggccatgac acatctttgt ttcagtaccg aaaagtcatg gatggccggc
      121 acaaggccga ccagtacaca agatcaaccg gttcgaagcg tatcgtcgcc gaaacctgat
      181 tctgacctca ccaagcgtgc cctcggttgc tgctgcccgc gcccgtttcc gatcgatcgg
      241 gaaatctggc aggagggatc gtaggctgcc aaaaacgcca cctacctgcc gggcctaggc
      301 gatgcccgct tccggatcgc acgcggtggc cggttcaagc ttcctgccga tgaaccatga
      361 caggaatgtt cttggccgcg ggtgtcttgc tgcctttcgc atggtgccat agcggcagca
      421 gcacattggc ttcgggatag taagctccga tgcagccgac aggaatgtcg tagggcgtta
      481 cccggagccc catcaactcc cggtggacgc catcgtcggt ggaggtcgtc agcttcacgt
      541 tttcaccttc ccgcagcccc agccgcgcga tgtcgttccg gttcatgaag acgatcatgc
      601 gggtcccctc tacaccgcgg aaccggtcgc tgtagccata aaccgtcgtg ttgaactggt
      661 cgttgcttcg cagcgtcatc aagcgcagga cgtcgtgacc aatctccggc atatccaggt
      721 cttcttccag ggcggttggc gtgatgatgt tggccttccc agtcttggta tgccaaaccc
      781 gctcgcgggc aggcaggtag cgatggaagc cgcccggttc ccacatccgg cgttcatagt
      841 catgaaagat gtcgggatag gtttcggcga tcgagaggcg gatgcgactg taatccgaca
      901 tccaagcgtc ccagggcact ttcggattgg gatccagcgt cgcctttgcc atcctggcca
      961 gcacctccac ttcgctcagc aggaacttgg atgccggcgc gcgaactccg cgcgaaccat
     1021 ggatgcaggc tgacgtatcc tccatcgaca gggcctgcgg ctcgccgccc tgcttgtcca
     1081 cctcgatccg gctcttgacc ggcaggatgt agctgatttc tccgtgcacg aggtgactgc
     1141 ggttgagctt ggtctgcaca ttcactgtca ggcggagctt gcgccaggct ggctccatca
     1201 gtccccggtc cggtacggca cggatgaagt tcccgccgaa catgacgaag gcgcgaacct
     1261 tgccgtgcag aatcccctcg cacgcctcga cggtgtccat gcccttttcc cgcggcggct
     1321 cgaatccata ctgcgccgca agtcggtcga gcggcaccag ctccggcttt tcggtgatgc
     1381 ccacggtgcg ctgtccctgc acgttcgagt ggccacgcac cgggcagatt ccggcaccct
     1441 gcttgccgat gttgccgcgc agcagttgca gattgaccag catctggatc gtctcgacac
     1501 cggcctgatg ctgggtgagg cccatgccat agatcgcgat cgtggcattg gaccggcagt
     1561 aagtcatggc cgtcgcctcc agtgcggcac ggcggaggcc ggaacgacgt tccagctcgt
     1621 cccagtcctg ctgcgccagc catttggcga agcttgcgaa accgtcggta tgttctgtga
     1681 tgaaggcgtg gtcgatcaca cgctgctggc cactggcttc ggcagccgtg tcaagctcga
     1741 caaccgcttt gcatatgccg gcaagggcgg ccagatcgcc gccggtcttg acctgatgat
     1801 aactggaact gatccgggtt gagctgccgg tcagcatctc gacgggcgac tgcgggttgg
     1861 taaagcgttc aagtccacgc tccggcagcg gattgaacgt gatgatcggg acaccgcgct
     1921 tactggcttc ctgaagcgga tgcagcatcc gcgggctgtt gctgccggga ttctggccaa
     1981 agaacaggat gcactcggca tgggcgaagt cgtcgaggct tacggtgccc accggcacgc
     2041 cgatgctttc cggcagcgcg accgaggttg tctcgtggca catgttggaa ctgtcgggca
     2101 ggttgttgtt gccatacaac cgggcgaaca aaccatacag gtaactcgtc tcgttcgaca
     2161 tgcggccgga gctgtagaac acggtctgct tcgggtccgg gaccgcccgc agttcgcgtc
     2221 cgatttcagc gaaggcgtcc gcccaggaaa ccggcaggta tttgtcggtg gcggcatccc
     2281 agcgcagcgg gtgggtcagc cggccggctt gctccaggct gtagtcgtcc cagtccatca
     2341 gttcggtaac tgtgtgcttc gcgaagaagt ccggagtaca gcgatgactg gtgatgtccc
     2401 aggcggtggc cttggcgccg ttctcgcaat actcgaacgg tagtggatcg gctggcttga
     2461 tccaggcgca actggtgcat tgatagccat ccgccttgtt gtggcggagc aggaccaagg
     2521 gaccgcttgc gagcacgcct tcgcgccgca tgatattgcc gagcgatcga gcagaacccc
     2581 agccgccagc aggcgccgta taaggttgga aagtagggtg tttgcccatg cgtttgctcc
     2641 atccgaattc ggcacctcgt attgtgcccc aaatacaaaa gcccaccggc gcaacacggt
     2701 tgcttcaagt cggaacccgc gtggtgacat tcgtgtttgg ctcgtgcatc gagcgagagg
     2761 aaagatcatg gtggacagga attacctgct caggaagccg cccccgccgc cgccctggaa
     2821 gcagtttctc gacctgacgg ttctgccggc gatgatcgat gccgccggtg cggtggaagg
     2881 attgctcgaa cgggtggccg tccagggtcg gcagcgaccc gccttgacga ccggtatggc
     2941 ccttggactg gcttgtgggc ttgccctgct ggttggccct cgtaagtccc gaaattaagc
     3001 cactacggcg cccggccgca ccgcctgtgc tccgaaagac cattttcggc ctgacggtac
     3061 aggccgaaaa tggtgacagg ccgccaatgg tctttctcct tggttgatcg cataaatcac
     3121 gcgcttcgga cgttccgccc ggcgtgatca cgctcgaact tccagttcgc caggatctcg
     3181 tccgtgctcg cggtctccac ctgctggcca tagcggccat tgtaaagctg caaggatgca
     3241 tcgtgggttt cgtccgcgga actgcagatc gcatcgttcg ccacgataac gcggtagccg
     3301 cggtccacgg ctccgagcac ggtcgcgagg acgcagacgt cggtttcgct gccgctgacg
     3361 atgatggtat ccgcgcccaa tgcctcgatc cgggactgca atccggaatc cacccaaggc
     3421 gaataaaccc gcttgtcaac gacctgcgcc ggtgggacca gtgcggcgag ttcggggacg
     3481 agctcgacca tttcccggcc gaggcgctcg atcgtcatcg acgcccagcg ttcatagtaa
     3541 cgcttccagg ttccgctgcc ttcgccagcg cgaaaagccg ggatgaacct ggtgaagatg
     3601 gtgcgttcgg gatgggcttc cgcgatacgc cgcgcgagcg gtagcacccg cgacatccag
     3661 ggggtatgcc aatcggtatt ctcggcaaac aggcgctgca tgtcgcagca taggtgcacg
     3721 cacgccgatc caatatcgcc atgtcgcagc gatgcgggaa gatcgccggg cggagcctca
     3781 ctcgaactca agctgtgtcc tccgctgccg gtttgatttc gcggcgcagt aactcggccg
     3841 aagcggat
//
LOCUS       DASWQN010001195         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_512375, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001195 DASWQN010000000
VERSION     DASWQN010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512375"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1460)
                     /locus_tag="VGC82_20265"
     CDS             complement(159..1460)
                     /locus_tag="VGC82_20265"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0425664.1"
                     /translation="MMVRPDLHLPQAFVVWMAPFLAAFCQRSRDTAAALAVGALLAIG
                     PRTVSTCLRALGLAEHPSFTAFPRVLNRNVWSSLAVARTLLQQLVPVFEPGRPFVIIG
                     VDHPLERRRGRRIEPASHFHDAVRSTAKQKVTSRGLRWISAMLLVNVPFAGRIWGLPV
                     LTALTPSKAWCESHKRRYRPVTEWAERLLLTLHRWLPDRVIVAVMDGEFAAIDLLHRL
                     CRSMVVITRLRLDASLFDPPAAYKGRGRYPKKGARQPSLKARITDRATVWKRAVQASR
                     TSWRSGGWIDYACGTALWYHAGKQPLPIRWILVRYPDKRHDTEAFLCTDTEMAPLDVL
                     DCYNRRWSMETTYQEARAHLGVETQRQWSDPAIFRTTPLLFGLYSLVSLYVQQNAERL
                     ALSPRRAAWYPKPAATFADALARLRQHVWFERLPRQPMPPT"
     gene            1522..1800
                     /locus_tag="VGC82_20270"
     CDS             1522..1800
                     /locus_tag="VGC82_20270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425665.1"
                     /translation="MSSIDPVIERIVVYPWPRGGVSVRKLRGGYSLSSARTGAPVARL
                     KPMPPDDRFEVLWWRREAWGPAGPFGAVLPFGDALDFIASESFFWINA"
BASE COUNT          339 a          569 c          651 g          323 t
ORIGIN      
        1 catcgcgggc cagtgatgtc gaggttgaat tcgacattcc atctgagcca cgccgcttgg
       61 gatgccgctt cgccgccgct gaccggctga agtgtcagtt cagggcgcgt agcacgcgag
      121 ctggatcaat cggtgcagat caggcgggag gaattccttc atgttggggg catcggctga
      181 cgtggcaaac gctcgaacca gacatgctgg cgcagacgcg ccagagcatc ggcgaaggtc
      241 gccgccggtt tgggatacca ggcggcccgg cgtggtgaga gtgccagccg ctcggcgttt
      301 tgctgaacgt agaggctgac caatgaatac aggccgaaca gcagcggcgt ggtgcggaag
      361 atcgccggat ccgaccactg acgttgggtc tcgacgccga gatgagcccg cgcctcttgg
      421 taggtcgttt ccatcgacca gcggcgattg tagcagtcaa gcacgtcaag cggggccatt
      481 tcggtgtcgg tgcaaagaaa ggcctcagtg tcgtgacgtt tgtccgggta acgaaccaat
      541 atccagcgaa tgggcagcgg ctgcttgccg gcgtggtacc acagcgccgt tccgcaggca
      601 tagtcgatcc agccaccgct gcgccaggat gtgcggctgg cctgcaccgc ccgcttccag
      661 actgtggcac gatcagtgat gcgcgccttc agcgacggtt ggcgcgcacc tttcttgggg
      721 tagcggccgc gtcccttgta ggccgcggga ggatcgaaca ggctggcgtc cagccgcagt
      781 cgggtgatca caaccatgga tcgacacagc cggtgcagca gatcgatggc ggcgaattca
      841 ccgtccatga ccgcgacgat cacgcggtcc ggtagccagc ggtgcagcgt cagcagcaga
      901 cgctcggccc attcggtcac cggccgatag cgccgcttgt ggctctcgca ccaggccttg
      961 gacggggtca gcgcggtcag caccggcagt ccccagatac gaccggcgaa cggcacgttg
     1021 accaacagca tcgcgctgat ccaacgcaga ccccggctgg tgactttctg tttggcggtg
     1081 gagcggacgg catcgtggaa gtggctggcg ggctcgatgc gacgaccgcg gcggcgttcc
     1141 agcggatgat cgacgccgat gatgacgaag gggcggcccg gttcaaagac cggaaccagc
     1201 tgctgcagca aggtccgggc cactgccaag ctggaccaaa cattgcggtt gagcacgcga
     1261 ggaaaggcgg tgaagcttgg atgttcggcc aggccgaggg cgcgcaggca ggtgctgacc
     1321 gtgcgcggcc cgattgccag cagcgcgccg acggccaggg ccgcggcggt atcgcgactg
     1381 cgttgacaga aggcggcgag gaagggcgcc atccagacaa cgaaggcttg cggcagatgc
     1441 agatcggggc gaaccatcat cgatctcctg cgacggtacg gggccggcag gtcaaacagc
     1501 gcggcgctcg ggaagatcgg tgtgtcctca attgatccgg tgatcgagcg gatcgtggtc
     1561 tatccctggc ctcgaggcgg cgttagcgtc cgcaagctgc gtggcggcta tagcttgtca
     1621 tctgcccgca ctggcgcccc cgtcgctagg ctgaagccaa tgccgccgga cgatcgcttc
     1681 gaagtactgt ggtggcgccg cgaggcttgg ggtccggccg gaccgttcgg tgccgtcctc
     1741 ccattcggcg atgctctcga tttcatcgcc tcggagtcat tcttctggat caacgcctga
     1801 tctcaccggc ccgtgatggg caaagtccag gagatcgatc atggcatagg tcatgcgcac
     1861 cacctcgctc cgggtcggcg cg
//
LOCUS       DASWQN010001196         8070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2097022, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001196 DASWQN010000000
VERSION     DASWQN010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097022"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..127
                     /locus_tag="VGC82_20275"
                     /pseudo
     CDS             56..127
                     /locus_tag="VGC82_20275"
                     /inference="COORDINATES: protein motif:HMM:NF015185.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            207..365
                     /locus_tag="VGC82_20280"
     CDS             207..365
                     /locus_tag="VGC82_20280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425666.1"
                     /translation="MANPATGYSVVYHIEIALLFATLIAIGPLVRRPGLPGFRLGELS
                     SHSLSLPR"
     gene            370..1134
                     /gene="puhA"
                     /locus_tag="VGC82_20285"
     CDS             370..1134
                     /gene="puhA"
                     /locus_tag="VGC82_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009521 - photosystem [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center subunit H"
                     /protein_id="HEY0425667.1"
                     /translation="MQVGAITGYIDVAQIALYVFWLFFAGLIYHLRREDKREGYPLVT
                     ERPGQYLQGFPVMPPPKTYLLVEGGTVSLPRVDREEPPYNAQPVAAWPGAPLQASGNP
                     MLSGAGPAASALRAEVPDLLQENGDNRIVPLRVATDHFLDDESPDPRGKEAIGADGAV
                     GGIVSDLWIDRAETAIRYIEVTLAAGASVLVPMPLAKVATNGRVMVSSVMGAQFADAP
                     ALANPDQITLREEDRIQAYFASGHLYAEPGRAEPLL"
     gene            1131..1784
                     /gene="puhB"
                     /locus_tag="VGC82_20290"
     CDS             1131..1784
                     /gene="puhB"
                     /locus_tag="VGC82_20290"
                     /inference="COORDINATES: protein motif:HMM:NF040894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030096 - plasma membrane-derived
                     thylakoid photosystem II [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic complex putative assembly protein
                     PuhB"
                     /protein_id="HEY0425668.1"
                     /translation="MNEHDYEPVPGLPAPLPQGETILWQGAPRWETFARRAMRVPLVA
                     GYFAVLAVWGVADRLSSGMAATDILLSTLRLAGLAVVAIALLELFAWLVSRTTLYTIT
                     TRRVVIRFGIALPITIQIPFRMIASAGARVWSNGTGDIALQLLPGQRIAYLLLWPHAR
                     PWKLMTAEPAMRGIPDAAMVAQILGRALSASASQPAKAVTIPVTASNGAGGHVPAAA"
     gene            1796..2239
                     /gene="puhC"
                     /locus_tag="VGC82_20295"
     CDS             1796..2239
                     /gene="puhC"
                     /locus_tag="VGC82_20295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic complex assembly protein PuhC"
                     /protein_id="HEY0425669.1"
                     /translation="MSTAPFPRGILALAGSAVALSIVAAAVGRLTGAADSTPTAAMVA
                     SRELLFRDQPNGAVVVYDASDTSAPIEVVAPATNGFLRATMRGLARQRIRQDSDREIP
                     FRLTGWADGRLTLEDPTTGRKVEMEAFGITNEEVFAHLLTARVGS"
     gene            2236..2514
                     /locus_tag="VGC82_20300"
     CDS             2236..2514
                     /locus_tag="VGC82_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425670.1"
                     /translation="MRRIEVPCLLDIEQTPQSLHAHAVPEGIEIQPGDKVLIHGAPTK
                     IGYGERISLRCSATVIRATALRRLWTQFTGLLEFTALYEVGFERRERS"
     gene            2511..3557
                     /gene="acsF"
                     /locus_tag="VGC82_20305"
     CDS             2511..3557
                     /gene="acsF"
                     /locus_tag="VGC82_20305"
                     /EC_number="1.14.13.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048529 - magnesium-protoporphyrin IX
                     monomethyl ester (oxidative) cyclase activity [Evidence
                     IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-protoporphyrin IX monomethyl ester
                     (oxidative) cyclase"
                     /protein_id="HEY0425671.1"
                     /translation="MKPVSETTKLAQRDTVLSPRFYTTDFAAMDKLDVTPVRREWDEL
                     MAEFARDTNKHHFVRNADFDMDLSTMPEDLREEFIHFLVSSVTSEFSGCVLYAEIKKR
                     VTNPDIRDWFAYMSRDEARHAGFINDTLKDFGVGVDLSFLTKVKKYTYFQPKFIFYAV
                     YLSEKIGYARYITIFRQLERNPDKRFHPIFKWFKEWCNDEFRHGEAFALIMRANPEIL
                     TGFNRLWIRFFLLAVFATMFVRDHGRPVFHGALGLDPTRYDMDVFRITTEISKQVFPV
                     TLDVDNPAFLAGLQRLLRISQGLDAAKGQFGLGGILKGLGLRASAALTLGRLFLMRPQ
                     RHELPREIRVAPAW"
     gene            3565..4394
                     /gene="puhE"
                     /locus_tag="VGC82_20310"
                     /pseudo
     CDS             3565..4394
                     /gene="puhE"
                     /locus_tag="VGC82_20310"
                     /inference="COORDINATES: protein motif:HMM:NF023711.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative photosynthetic complex assembly protein
                     PuhE"
     gene            4391..5602
                     /gene="hemA"
                     /locus_tag="VGC82_20315"
     CDS             4391..5602
                     /gene="hemA"
                     /locus_tag="VGC82_20315"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEY0425672.1"
                     /translation="MNYETFFRKQIDGLRREGNYRVFADLERQAGKFPQAIHHKERGK
                     APVTVWCSNDYLGMGQHPSVIAAMHEALDRCGAGAGGTRNIGGTNHYHVMLEHELADL
                     HRTEAALLFNSGYMSNWATLSTLAGRIPGCVVLSDESNHASMIEGVRHSRAEKRIWAH
                     NDPEDLRRLLSELDPERPKLVAFESVYSMDGDIAPIAEICDVAEEFGAMTYLDEVHAV
                     GLYGQRGGGVSEREGVAHRLTVIEGTLAKAFGVVGGYIAGSATMCDFVRSFASGFIFT
                     TALPPSVAAGAVASIRYLKTSHTERSRQREQVAKLRRRLEAIGVPHYDNPSHIVPVMV
                     GDPVMCKEISDRLLDEFAIYVQPINYPTVPRGTERLRITPSPLHSDSDIEDLVNALGT
                     IWSEMSLRRAA"
     gene            5657..5914
                     /locus_tag="VGC82_20320"
     CDS             5657..5914
                     /locus_tag="VGC82_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425673.1"
                     /translation="MRTTDDTTRRRVLRAGLAMAGGVVAARAEAQEKLAPALVQYQTT
                     PKDGNKCSACVNFEAPKACKIVSGTIEPEGWCVAFAPKEAK"
     gene            complement(5997..6989)
                     /locus_tag="VGC82_20325"
     CDS             complement(5997..6989)
                     /locus_tag="VGC82_20325"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEY0425674.1"
                     /translation="MDSMEVNKAMAAILVAGIAFFLTGTAGTVLVREHKLEKPAIKID
                     VPEAAPSSGQPAPVTLPPVANLLAKADVAAGQAYASKVCSACHTFTQGGKAGVGPNLY
                     NVVGGPHGHMEGFNYSAALKGKTGPWTFDALNEWLHKPSTYAPGTRMAFAGISDDQER
                     ANVIDYLHTLSPNPVPLPAPVAAQAAAPAQAAAPPAAANNATPPAQAGDPPIETLLAS
                     ADAKEGEGDTKKLGCVACHTFNEGGKAGLGPNLYGVVGAPHGHMSGFNYTAVLKSKQG
                     PWTYDELSEWLKQPNAYAPGTRMSFAGIKDAKERADVIAYLRSLSPNPEPLPAK"
     gene            complement(7059..>8070)
                     /gene="pufC"
                     /locus_tag="VGC82_20330"
     CDS             complement(7059..>8070)
                     /gene="pufC"
                     /locus_tag="VGC82_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="photosynthetic reaction center cytochrome PufC"
                     /protein_id="HEY0425675.1"
                     /translation="LALRIAAGGAIVATAAVVLVTSERPPVEGIQRGYRGVAMQQIYN
                     PRIVADELAANQIPASLPRLPDAGPKAGAVYKNVQVLGDESVGNFTRLMASITQWVSP
                     TQGCAYCHNVNNMAEDSVYTKVVARRMIQMVQHVNADWKPHVAETGVTCYTCHRGQPV
                     PANIWFHDPGPTQAGGFAQAPAGKNHPTTVANNSSLPFDPFTPFLEQDDNNIRVQSTE
                     ALPSGDRQSIKQTDWTYSLMIHFSQGLGVNCTYCHNSRSWSDWNQSTPQRATAWYGIR
                     MVRDLNVNYLNPLQSTLPPARLGPEGDSPKVNCATCHNGVYKPLFGVSMAKDFPELTV
                     AH"
BASE COUNT         1412 a         2431 c         2627 g         1600 t
ORIGIN      
        1 cgcgttgatc ggtttcggcg cgggcttgtt cgcccactgc accctgaccg ccgccatggg
       61 cacagcgcga agcggccaga tcggcctggc gctgggtgtc tggggtgcgg ttcaggcttc
      121 tgccgcaggc accgccgttg ccgccggagg ccttattcgc gacggtgttt ccgccctcgc
      181 gacggccggc gtgctgggtc aaggcgatgg ccaacccggc gacgggatat tccgtcgtct
      241 atcacatcga aattgcgctg ctgttcgcga cgttgatcgc catcggtccg ctggtgcggc
      301 ggcccggact gccaggcttc aggcttggcg aactatcctc ccactcgctc agtcttccga
      361 ggtgatgtca tgcaggtcgg agctatcacg ggttacatcg acgtggcgca gatcgcgctt
      421 tacgtattct ggttgttctt cgccggactt atttatcacc tgcgcaggga agacaagcgg
      481 gagggctatc cgctggtcac tgaacgccca gggcaatacc tgcaaggttt cccggtgatg
      541 ccgccaccga agacctacct gctggtcgag ggaggcacgg tatccctgcc gcgggtcgat
      601 cgggaggagc cgccatataa cgcccaaccg gtcgcggcgt ggcccggcgc accgttgcag
      661 gcttccggca acccgatgct gtccggcgcc ggaccagcgg catcggcgtt gcgtgccgag
      721 gtacccgacc tgttacagga aaacggtgac aaccgcattg ttccgttgcg cgtcgcgacc
      781 gaccatttcc tggacgacga gagccccgat ccgcggggta aagaggcgat cggcgcggat
      841 ggcgcagtcg gcggcatcgt ctcggatctg tggatcgatc gcgccgaaac ggccatccgc
      901 tatatcgagg tgacattggc cgcgggggca tcggtgttgg tgccgatgcc cctcgccaag
      961 gtcgcaacga acggccgcgt catggtttct tcggtgatgg gcgcgcagtt cgccgatgcg
     1021 ccggccctgg cgaatcccga ccagatcacc cttcgtgagg aggatcggat tcaggcctac
     1081 ttcgccagcg gtcatctcta tgccgaaccc ggccgggcgg agccactgct atgaacgagc
     1141 acgactatga gccagtaccg ggacttcccg ccccgttgcc ccaaggcgaa acgatccttt
     1201 ggcagggcgc accgcggtgg gaaacgtttg cccgccgagc gatgcgagtg ccgctggtag
     1261 cggggtattt cgcggtgctg gcggtatggg gcgtcgcgga ccggctgtcg tccggaatgg
     1321 ccgccaccga tatcctgttg tccacgcttc gcctcgccgg gctcgccgtc gtcgccatag
     1381 cgctgctgga actatttgcc tggctggtgt cgcgcaccac gctctacact atcaccacgc
     1441 gccgggtggt gatccgcttc ggcattgcac tgccgatcac catccagatc ccgtttcgga
     1501 tgatcgcctc cgccggagcg cgcgtttggt ccaatgggac cggcgacatc gcgttgcagc
     1561 tgctgccggg gcagcggatc gcatatctgt tgctctggcc gcacgctcgg ccatggaagc
     1621 ttatgactgc cgaacccgcg atgcgtggca tccccgatgc agcgatggtt gcccagatcc
     1681 tggggcgggc gctttcggcg tccgcgtcgc aaccggccaa ggctgttacg ataccggtca
     1741 ccgcatcgaa tggcgccggc gggcatgtcc cggccgcggc ctgacggaga cgatcatgag
     1801 caccgctccg tttccccgtg gcatcctggc gctggccggt tccgcggtcg ccttgtcgat
     1861 cgttgccgcc gctgtgggac gtttgaccgg cgcggccgac agcacgccga ccgcggcgat
     1921 ggtcgcatcc cgcgaactgt tgttccgcga tcagccaaac ggtgccgtgg tggtgtacga
     1981 tgccagcgat acctcggccc cgatcgaagt cgtcgcaccc gcgaccaacg ggttcctgcg
     2041 ggctaccatg cgtgggctgg cgcggcagag aatacggcag gattccgatc gggagattcc
     2101 cttccgcctg accggctggg ctgacggccg cctgacgctg gaagacccca cgaccgggcg
     2161 aaaagtcgaa atggaagctt tcggcatcac caacgaagag gtcttcgccc acctgctgac
     2221 cgcgcgggtc ggatcatgag gcgtatcgaa gttccgtgcc tgctggatat cgagcagact
     2281 ccacaaagcc tgcacgccca cgcggtgccg gaaggcatcg agatccagcc gggcgacaag
     2341 gtgttgatcc acggggcgcc aaccaagatc ggctacggtg agcgaatctc gctgcgctgt
     2401 tcagcgactg tgatccgggc cacagcgttg cgccgcttat ggacgcagtt taccggtctt
     2461 ctggaattta cggcgctgta cgaggttggg tttgagcgga gggagcggtc atgaagccgg
     2521 tcagtgaaac gacgaagctg gcacagcgcg acactgtgct aagcccgcgg ttctacacca
     2581 ccgattttgc tgcgatggat aagctggacg tgacgccggt ccgccgcgag tgggacgagc
     2641 tgatggcgga gttcgcgcgc gacacgaaca agcatcattt cgtccgcaat gcggatttcg
     2701 atatggatct ttccaccatg ccggaggatc tgcgcgagga attcatccac ttcctcgtca
     2761 gttcggtcac ctcggaattt tccggctgcg tgttgtatgc cgaaatcaag aagcgcgtca
     2821 cgaacccgga catccgcgac tggttcgcct acatgagccg cgacgaggcg cgccatgccg
     2881 gctttatcaa tgacacgttg aaggattttg gtgtcggcgt ggacctgagc ttcctgacca
     2941 aggtgaaaaa atacacctat ttccagccca agttcatctt ctacgcggtc tatctgtcgg
     3001 aaaagatcgg ctatgcccgc tacatcacga tcttccggca gctggaaagg aacccggaca
     3061 aacggttcca tccaatcttc aaatggttca aggagtggtg caacgacgag ttccgccacg
     3121 gcgaagcctt cgcactgatc atgcgcgcga acccggaaat tttgaccggc ttcaaccggc
     3181 tgtggattcg ctttttcctg ctggccgtct tcgcgacgat gttcgtgcgc gaccacgggc
     3241 ggccggtgtt tcatggcgcg ctgggcctcg acccgacgcg ttacgacatg gatgtgttcc
     3301 gcatcaccac ggagataagc aagcaagtct tccccgtcac cctggacgtg gacaatccgg
     3361 cgttcctggc agggctacag cggctgctgc gcatcagcca ggggttggat gcggccaaag
     3421 gacaattcgg tctcggcggc attctgaagg gcctgggact gagggcctcg gcggctctga
     3481 cccttggacg cctgttcctg atgcggcctc agcggcatga actgccgcga gagatccgcg
     3541 tcgcgccggc gtggtaagcg gcaaatgctg caatacggac tcccggcgct gcacggcctg
     3601 ctgatctggt ggttcagcac tgggctgatc ctgttgctgg acgggctgcc ccgccatacg
     3661 ttccgctgga gcatggccgc cgcgacggcg ttgctgttcg cctcggtggg tggattgatc
     3721 gccagcgcgt ccgacacgag cgtcaccggc gcatactgca cctttacctg cgcggtggtg
     3781 atctgggggt ggttggaaat cagcttctac atgggatacg tgaccggtcc gcggacgcat
     3841 cactgccgcc atggatgcag cggctggcgc catctgggtc acgccgtcca ggcctgcctg
     3901 tatcacgaga tcgccgtact tgctttggcc ggcatcgtca ttggcctgac ctggtccagc
     3961 ccgaaccagt tcggaacgtg gacattcgtg gttctgtggt ggatgcacca gagcgcgcgc
     4021 ctgaacgtct ttctgggcgt cagcaacctg aatgccgagt tcctgccgga gcattggcac
     4081 atatccgaag cttctttcgc agtgcaccga tgaacctgtt gtttcccttt tcagtcacgc
     4141 tctccaccat cgccgccgtc ctgcttgggc gacaggcatt ggacaacgcg gggaatgcct
     4201 atctggccgt cggctatacc cttgtgacca cgatgatggt actggccatt ctggagcatt
     4261 ggttcctggt tctgccgctg tcggccgcga agctgtggaa cggtctttgg caatggagcc
     4321 tggggccgcg ccgcgcgaac ccgaagacgc cgcggccact gccaagacag gcaacgatcg
     4381 gaggacgtcc atgaactacg aaactttctt ccgcaagcaa atcgacggcc ttcgccgcga
     4441 gggcaactat cgggtgttcg ccgatctgga acgtcaggcc ggaaaatttc ctcaggcgat
     4501 ccatcacaag gagcgcggca aggctccggt cacggtgtgg tgctccaacg actatctagg
     4561 catgggacag catccgtcgg tcatcgcagc gatgcacgag gcgcttgacc gatgcggcgc
     4621 cggtgccggc ggcacgcgca acatcggcgg caccaaccat taccatgtga tgctggagca
     4681 tgaactggcg gacctgcacc ggacggaagc agcgttgctg ttcaactccg gctatatgtc
     4741 gaactgggcg acgctgagca cgctcgccgg ccggataccc ggttgcgtgg tgctgtcgga
     4801 cgagtccaac catgcctcga tgatcgaagg cgtgcggcac agtcgggctg aaaagcggat
     4861 atgggcgcat aacgatccgg aggatttgcg gcggcttctg tcggagctcg atcccgaacg
     4921 gccgaagctc gtggcgttcg aatcggtgta ttcaatggat ggcgacattg cgccgatcgc
     4981 cgagatctgc gacgtggccg aagaattcgg cgcgatgacc tacctcgatg aagtgcatgc
     5041 ggttgggctg tatgggcagc gcggcggcgg cgtcagcgaa cgtgagggcg tcgcgcaccg
     5101 gctgaccgtc atcgagggta cgctggccaa ggccttcggc gtggtaggcg gctatatcgc
     5161 cggatcggca acgatgtgcg acttcgtgcg cagcttcgcc tcggggttca tctttaccac
     5221 ggcattgccg ccttcggtcg cggccggcgc cgtcgcaagt attcgttact tgaagacaag
     5281 tcataccgaa cggtcgcggc aacgggaaca ggtggcaaag ttgcgccggc ggttggaagc
     5341 gattggcgta ccgcattacg acaaccccag ccacatcgtt ccggtcatgg tcggagatcc
     5401 tgtgatgtgc aaagagatca gcgaccgttt gctggatgag ttcgcgatct atgtacagcc
     5461 gatcaactat cccacggtgc cacgaggcac cgagcggctg cggatcacgc cttcgccgct
     5521 gcactccgac tcggacatcg aagatctggt gaacgcgctc ggtaccattt ggtccgagat
     5581 gagcttgcgc agagccgcat gaccaccaga tttccacgcg gggcagattt ccacgagggc
     5641 agagaaaggg actaatgtga ggacaacgga cgatacgacg cggcggcggg ttttacgggc
     5701 cgggctggcg atggctggcg gcgtggttgc tgccagggcg gaagcgcagg agaaacttgc
     5761 gccagcgctg gtgcagtatc agacgacgcc gaaagacggc aataaatgca gtgcgtgtgt
     5821 gaacttcgag gccccgaagg catgcaagat cgtgtcggga acgattgaac ccgaagggtg
     5881 gtgcgttgcc ttcgcgccaa aggaggcaaa gtagtccaaa ggaggcaagt agtccaaggc
     5941 atcgtcccgg cgggtgcttt ggcccgtcgg gacgtcctgc ttcgcggggg gacgtcctac
     6001 ttcgcgggta agggttcggg gttcggcgac agcgatcgaa gatatgcgat cacgtcggcc
     6061 cgctctttcg catccttgat cccggcaaac gacatccggg ttcctggagc ataggcattc
     6121 ggctgcttaa gccactcgct gagttcgtcg tacgtccacg ggccctgctt gcttttcagt
     6181 acggcggtgt agttaaaccc tgacatgtgc ccatgtggcg cccccacgac gccatacaag
     6241 ttcggaccga gaccagcctt gccgccttcg ttgaacgtgt ggcaagcgac gcagccgagt
     6301 tttttggtgt cgccctcgcc ctccttggcg tcggccgagg ccagcaacgt ttcgatgggt
     6361 ggatcgccgg cctgggccgg tggcgtggcg ttgttcgcgg cagccggtgg tgccgcagcc
     6421 tgtgcggggg cggcggcctg tgccgctaca ggcgcgggca gcggcaccgg atttggcgac
     6481 aaggtgtgca ggtagtcgat cacgttggcg cgttcctggt cgtcgctgat gccggcaaac
     6541 gccatgcgcg tcccgggcgc gtatgtgctt ggcttatgca gccactcgtt caacgcgtcg
     6601 aatgtccatg ggccggtctt gcccttcaac gccgcggagt agttaaaccc ctccatgtga
     6661 ccgtgcgggc cgccgacaac attgtaaaga ttgggtccca cccctgcttt gccaccctgg
     6721 gtaaaggtat gacaggcgga gcaaaccttg ctggcgtagg cttggcccgc ggctacgtcg
     6781 gcctttgcaa gcaggttcgc gactggtggc aacgttaccg gggccggttg cccggaggac
     6841 ggcgccgcct ccggcacgtc gatcttgatc gcgggttttt ccagtttgtg ttcccggacc
     6901 aacaccgtgc cggctgtccc ggtcaggaag aaggcgatcc cggcgaccag gatcgccgcc
     6961 atggccttgt tgacttccat cgaatccatt caggcttggc tcctggtcac cgggttagct
     7021 gaagttcccg gcagatatcg tcgcggcgcc caattttcct agtgcgccac ggtcaattcc
     7081 gggaagtcct tcgccatgct gacgccgaac agcggtttgt aaacgccgtt gtggcaggtc
     7141 gcgcaattca ctttcgggga atcgccttcg ggtccgagcc tggccggagg caaggtgctt
     7201 tgcaggggat tgaggtagtt gacgttcagg tcccgcacca tccggatgcc ataccatgct
     7261 gtcgcgcgtt gcggcgtgct ctggttccag tcggaccaag acctggaatt atggcaatag
     7321 gtgcagttga ccccgagccc ctgggaaaaa tggatcatca gggaatatgt ccaatcggtc
     7381 tgcttgatcg actgacggtc gccgctggga agagcctcgg tcgactgcac gcggatgttg
     7441 ttgtcgtctt gttccaggaa cggcgtgaat gggtcgaacg gcagggaact gttgttcgcc
     7501 actgtggttg ggtggttctt tccggccggc gcctgagcga aaccgccggc ttgcgtgggt
     7561 ccgggatcgt ggaaccagat attcgccggc accggctgac cccggtggca ggtgtaacag
     7621 gtgacgccgg tctcggcgac gtggggtttc cagtcggcgt tcacatgctg caccatctgg
     7681 atcatacgac gtgcaaccac cttggtgtac accgagtctt ccgccatgtt gttgacgttg
     7741 tggcagtagg cgcagccctg ggtcggggag acccattggg tgattgatgc catcagccgg
     7801 gtaaagttgc cgacactttc atcgcccagc acctgcacgt tcttatacac agcaccagcc
     7861 ttgggaccgg catcgggaag tcgcggcaga gaggcgggga tctgattggc cgccagctcg
     7921 tcggcgacga tgcgggggtt gtaaatctgc tgcatcgcca caccgcggta tccgcgttgg
     7981 atgccttcca ccggcgggcg ttccgacgtc accaggacaa cggcggccgt ggcgacaatg
     8041 gcgccaccag ccgcgatacg aagggcgagg
//
LOCUS       DASWQN010001197         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_512494, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001197 DASWQN010000000
VERSION     DASWQN010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512494"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VGC82_20335"
     CDS             <1..1059
                     /locus_tag="VGC82_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425676.1"
                     /translation="VGRIASHRGLGRSEAKRTEGRHGSENLAGSRALNGHRLAPGQAA
                     RQDNVMDMRMTKLMRPVLQMAGLFLTLINTSQADDQTFRQASIGFIKTYFQHWSSSNA
                     VALTYLGDVFPDRVTYFNKTLDHTALMNAKHRFMERWPERRFKERGDSLNVTCDEQHL
                     CTVWGLVDWECRSQARHESATGTSAFSFQLQDGMAMVAEDGFVLSRGQILSRHPPGAA
                     EASSNATAGSLAAQGAARASLNGRARDFTDDDIPGLRAAYFAQSSDRDWIKDWLLVQK
                     EFTGTARSLGPVGVQTLSGANGDDMHVIQFDTHQGPIACVTTDNVPSIAQGSDVRIHG
                     IVSIFIDQTMYLANCSFT"
     gene            complement(1104..1448)
                     /locus_tag="VGC82_20340"
     CDS             complement(1104..1448)
                     /locus_tag="VGC82_20340"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY0425677.1"
                     /translation="MTQQGEASYFNGGQNGHTRTANGEPVQPDRNTAASRTLPPGTNA
                     TVTNKATGQSTEVHVNDRGPVRQDRVIDLSKKAAGDVGMSKTGTAPVVVQADPNRQSD
                     PAVRQKLLEQGK"
     gene            1820..>2159
                     /locus_tag="VGC82_20345"
     CDS             1820..>2159
                     /locus_tag="VGC82_20345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY0425678.1"
                     /translation="MSGWRLTLLAALAGGMACAAAAEPLPANAPGARQEAERLNNLPP
                     VQPGRAGHVDQSGRKQKGGASVYAHRFANKKMANGRRMDPNSNMAASKTLPLGSVATV
                     TNLKNGKTATV"
BASE COUNT          438 a          634 c          683 g          404 t
ORIGIN      
        1 gtcggccgga ttgccagcca ccggggtctg ggacgctccg aggcaaagcg aaccgaaggc
       61 agacacggca gcgaaaacct tgccgggtcg cgcgcgttga atggccatag gctggcaccc
      121 ggccaagccg cgcgacaaga caacgttatg gacatgcgta tgacgaaact catgcgacca
      181 gtgttgcaaa tggcgggcct gttcttgact ttgatcaaca caagccaggc agacgaccag
      241 acgttccggc aggcttcgat cggcttcatc aaaacgtatt ttcaacactg gtcatcctcc
      301 aacgccgtgg ctttgactta tctgggcgat gtgtttcccg atcgggtcac gtatttcaac
      361 aaaacgctcg accacacggc cctgatgaac gccaagcatc ggtttatgga acggtggccg
      421 gagcgacgct tcaaggaacg cggcgatagc ctgaacgtca cttgcgacga acagcacctt
      481 tgcactgtat ggggactggt tgactgggag tgccgcagtc aggcacgtca tgaatccgca
      541 acgggtacct cggcgttttc ctttcaactg caagatggca tggcaatggt cgcggaagac
      601 ggtttcgttt tgtcgcgcgg ccaaattcta tcgcggcatc cgcctggtgc cgcggaagcg
      661 tcaagcaatg caacggctgg ctcgctggcg gcacaaggcg ccgcgcgcgc atcgttgaac
      721 ggccgcgccc gggactttac cgatgacgac attcccgggc taagggctgc ctatttcgcg
      781 cagtcgtcgg atcgcgactg gatcaaggat tggctcttgg tccagaagga attcaccggc
      841 acggcccgct cgctggggcc ggtgggcgtg caaaccctat ccggtgccaa cggcgacgac
      901 atgcatgtca ttcagttcga cacgcatcaa ggcccgatcg cgtgtgtgac gacggataat
      961 gtgccctcga tcgcccaagg atcggacgta cgaatccacg gtatcgtatc gatctttatc
     1021 gatcagacca tgtacctggc gaattgttca ttcacctgat caagatgtgc caggtcaccc
     1081 gcacctgatc aagatgcgcc gggtcacttg ccctgttcga gaagcttctg cctgaccgcc
     1141 ggatcggatt gccggttggg atcagcctgc acgacgaccg gcgcggtgcc ggtcttgctc
     1201 atgccgacat cgccagccgc cttctttgac agatcgatga cccgatcctg acgcaccggc
     1261 ccgcgatcgt tcacgtgcac ctcggtcgac tggcccgttg ctttgttggt aacggtggcg
     1321 ttggtcccgg gcggcaatgt gcgcgacgcg gcggtgttgc ggtcgggctg caccggttcg
     1381 ccgttggcgg tccgggtatg gccgttttga cctccgttga agtaggaggc ttcaccctgc
     1441 tgcgtcacgg gtccatccga cgacttcggt tgaccgagag cggcgcctgc aacaagcatg
     1501 aaacctacgg cgaaatggac ggtgcgcatg aggcttgaaa ttctccgttc gaaggtagcg
     1561 ccaccccggc taagtggcac gagttgatgt cagtaacggc aagccgcggc tccggttttg
     1621 taccggtggc gaaaagttgc cgcctgtccg gggacaattg acctggggcg gcttgcatcg
     1681 cattaacgcg cggtcacgca ggacggaatg tccaagagcg tgaatcctgc gatcacgccg
     1741 agcggcacgc acggcattgg ccggccggaa cccgggtcgc actccagcgg ttaccggcga
     1801 ttgattttga ggatgagaaa tgagtggatg gcgactaacg cttctggcag ccctggcagg
     1861 tggaatggcc tgcgcggctg cggcggagcc gctgcccgcc aatgcgccag gcgcgagaca
     1921 ggaggcggaa agactgaaca accttccgcc cgtgcaaccg gggagggcgg gccatgtcga
     1981 tcagtccggc cgcaagcaga aaggcggagc gtcggtttat gcccaccgct tcgccaacaa
     2041 aaagatggcg aacggacgcc ggatggatcc gaactccaac atggcggcga gcaaaacgct
     2101 gccgctgggc agcgtggcga cggtcacgaa cctgaagaat ggcaaaaccg caacggtga
//
LOCUS       DASWQN010001198         3097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_249542, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001198 DASWQN010000000
VERSION     DASWQN010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249542"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /gene="rutD"
                     /locus_tag="VGC82_20350"
     CDS             <1..765
                     /gene="rutD"
                     /locus_tag="VGC82_20350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HEY0425679.1"
                     /translation="YHEIHGRSGAPAVLLSSGLGGAAHYWAPQIPILAQHFRVIAYDH
                     RGTGRSGGTLPEGTTIGDMAAKAAALLDELAVETCHFIGHALGGLIGLQLALDRPKLL
                     DRMVLVNAWAKIHPHTLRCFAIRKSLLLDTGIEAYVRAQPLFLYPASWLADRQAWLAE
                     QDAAGVAHFPPVETVLRRIAAIEAFDVVANLADVATPALVVATNDDVLVPYQCSVNLA
                     ERLPDARLVLCDRGGHACNITDPDYFSETAAKFLLN"
     gene            938..2191
                     /locus_tag="VGC82_20355"
     CDS             938..2191
                     /locus_tag="VGC82_20355"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0425680.1"
                     /translation="MNAPVSFDHRMHAATAPLDQIDVSDPQLYCDDVWHPYFERLRRD
                     DPVHYVPDSPYGPYWAITKYKDIIQLEVNHKAFSSSWDLGGIRIENIPSGYERPSFIL
                     LDPPDHDQQRLVVSPTVNPINLAKMETLIRERTGQVLAALPRNEEFNWVDRVSIELTS
                     MMLCTLFDYPVEDRRQLVYWSEVVITDINAPGALVRSEAERFAIQLEYTERLNELWEE
                     RAKLPPSFDIISILAHSDLMNKMTLRERMGILTLFLVGGNDTTRNSMSGGLWGLSQFP
                     DQMRKLRANPGLVTDTVPEIIRWQTPVIHMRRTALEDVEFGGKTIKKGDKVVVWYISG
                     NRDDTAIDRPNEFIVDRRRPREHLSFGFGIHRCLGNRLAELQLKILWEEVIRRNLEIE
                     VVGKPVYAYSNFLRGIKSLPVRLAA"
BASE COUNT          619 a          961 c          958 g          559 t
ORIGIN      
        1 taccatgaaa tccatggccg gagcggagcg ccggcggtct tgttgtcttc cggtctcggc
       61 ggtgcggccc attactgggc gccgcaaatc ccaatacttg cgcagcactt tcgggtcatc
      121 gcctacgacc atcgcgggac agggcgcagc ggcgggaccc tgccggaggg caccacgatc
      181 ggcgacatgg cagccaaggc cgcggccctt ctggacgaac tggcagtcga gacatgtcat
      241 tttatcggcc acgcattggg cgggctgatc gggctgcaac tcgcgctcga ccgtcccaag
      301 ctgctggacc ggatggttct ggtcaacgcc tgggcaaaaa tccatccgca tacgctgcgc
      361 tgcttcgcga tccgcaaaag cctgctgctg gacaccggca tcgaggccta cgtccgggca
      421 caaccgctgt ttctttatcc cgcttcgtgg ctggccgacc ggcaagcgtg gcttgcggag
      481 caggacgcag ccggggtggc gcattttccg cccgtcgaga cagtcctgcg ccggattgcc
      541 gccatcgaag ctttcgatgt cgtcgcgaac ctggccgacg tcgcgacgcc ggccctggtc
      601 gttgccacga atgacgacgt gctggtcccc taccagtgct cggtcaacct cgccgagcgg
      661 ctgcctgacg ctcgactcgt gctgtgcgat cggggcggcc atgcctgtaa tattactgac
      721 ccggattatt tcagcgagac cgccgcgaag ttcctgctga actgaacact ccgtcgcgcg
      781 acaggtcttc tgcctcgcgc cgacgacgtc gtttgacatc cgcggcacag tcctatctaa
      841 aatcaaaacc gaggccgggc gaacgcggcc gtgataacgg ctgagctgcc aaagcccgat
      901 gtgccgtgca tgaaaaacaa gcagggaagg aaaaacggtg aacgctccag taagcttcga
      961 tcatcgcatg cacgcggcga ccgctccgct ggaccagatc gatgttagcg atccccagct
     1021 gtactgcgac gatgtctggc atccctactt tgaacggctg cgtcgcgacg atccggttca
     1081 ctacgtgccg gacagtcctt acgggccgta ctgggcgatc accaagtaca aggacatcat
     1141 ccagcttgaa gtcaatcaca aggccttctc gtcgagctgg gaccttggcg ggatcaggat
     1201 cgagaacatt ccgtccggct acgagcggcc aagcttcatc ctgctagatc cccctgatca
     1261 cgatcagcag cgcctggtcg tcagtccgac cgtcaatccg atcaacctgg cgaagatgga
     1321 gaccctgatc cgcgaacgga ccggccaggt tctcgcggcg cttccacgca atgaagaatt
     1381 caactgggtg gaccgcgtct cgatcgagct tacctcgatg atgctctgca cgctgttcga
     1441 ttatccggtc gaagaccggc gccagttggt ttactggtcg gaagtcgtga tcaccgacat
     1501 caacgcaccc ggcgcgctgg tgcggtccga ggcggagcgg ttcgcgatcc agttggaata
     1561 cactgagcgg ctgaacgagt tgtgggaaga acgcgcgaag ttgccgccga gtttcgacat
     1621 catctcgatc ctcgcgcaca gcgacctgat gaacaagatg acgctgcggg agcggatggg
     1681 catcctgacc ctgttccttg tcggcggcaa cgacacgacg cgcaattcga tgtccggcgg
     1741 tctttggggg ctgagccagt tccccgacca aatgcgaaaa ctacgcgcca acccggggct
     1801 tgtgaccgac acggtgccgg agatcatccg ctggcaaacg ccggtgatcc acatgcggcg
     1861 gaccgcgctg gaagacgtgg aattcggcgg caagaccatc aagaagggtg acaaagtcgt
     1921 cgtctggtac atctccggga atcgcgacga cacggcgatc gaccggccga acgaattcat
     1981 tgtcgatcgc cgccggccgc gcgagcacct gtcgtttggc ttcggcatcc accgctgcct
     2041 gggcaaccgg ctcgccgagc tgcaactcaa gatcctgtgg gaagaagtga tacggcgcaa
     2101 ccttgagatc gaggtggtcg gcaagccggt ctatgcgtat tcgaacttcc tgcgcgggat
     2161 caagtcgctc ccggtacgcc tggcggcctg acggctcgtc cgtctcgtat gcatatcttg
     2221 aataaagcac cacaggagtc ggcttcgaag taaccgcttc cggcgaggtc ggcagttgac
     2281 cacagatagc ctcatgaccg tgatgggggg ctacccacga cgtgcctgaa cgccagacat
     2341 tcgtgggtag ccggcacagg ccggtcagga caagagcaga aagatcacgc cacaagtaat
     2401 accggtagcc caaaggggcg attgaattga ccgactcaaa tggcagcggg atcaaacaac
     2461 gcgcgtagcc gccatgcaca gtcgttacgc catactcgaa gctcggctgc gcccggccat
     2521 ctctgccaga tagcgacagc gcaaggccag cattggtgat gccgggtctt ggttcggaca
     2581 tcgacgacac aagcggtctg aatgccccat tgcgcgcgta gcgccccagt gatcagcgac
     2641 agattgttgg cgaagcgcgc cacgatcacc tggctgacca cggacagccc ctcgggcggc
     2701 cggcgccgtt caggcggaac tggctgttgg catgctcgcc gcgctgatgc acctggccga
     2761 aactgcccgg acaacgcggg gggcaacaag accggatcgg gggcgcatcg gcacggaacc
     2821 gttccagcgc ttgcggggaa cgcgatagac gccggcgtcc gggctaaccc gcatttctca
     2881 cacgcattac gacggttgca ccatcgcaag cgctttcatt gcctgatcaa gctaacatgg
     2941 caaatggcgt ccaatgtgcc ggattttgaa cgcacgttcc cgccgccgcc cgtgggcgag
     3001 gttggtgggg aagtatgtgg ccttgcggtg tctggttcgt ggggctcagg cgccggcgcg
     3061 gcgcagcagg gcatgcgcca gggcaaactc atgctga
//
LOCUS       DASWQN010001199         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_644891, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001199 DASWQN010000000
VERSION     DASWQN010001199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644891"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(591..1133)
                     /locus_tag="VGC82_20360"
     CDS             complement(591..1133)
                     /locus_tag="VGC82_20360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0425681.1"
                     /translation="MIRLFAAVLGSVPFTPVGASPYHRETVTLVPPVASACVSSPFGP
                     RVLVNRPLAGTFHDGIDLPAPVGSTVSAVAPGRVIRVQRHGVGGLEVLIQHEGFIGVY
                     SHLGLIAPLLAEGGKTIYGGERIATVGRSGLTYGPHLYFGMIVDGRSVDPAPFLRVAA
                     CGSTVDHDGPLRPTRLFSQH"
     gene            complement(1312..>2868)
                     /locus_tag="VGC82_20365"
     CDS             complement(1312..>2868)
                     /locus_tag="VGC82_20365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY0425682.1"
                     /translation="AVALRGDAAAAAVFMDLLCGKEPDRHRPGGQHPDGAKWLSILNE
                     TGFLGRYLPDWARIVGQMQFDTYHIFTVDEHTIEAVRVLNTLERGELAEVAPIASELV
                     DHVQSRRSLYLAMLLHDIAKGRGGDHSALGAEVALDVGPSLGLTDEETETVSWLVLHH
                     LLLSATAFKRDIDDPKTILDLADTIQSPERLRLLLILTVADMRAVSSKVWNGWKATLL
                     RELYSRVAEVLAGGLATPERDVRVARAKQAVGGLLTDWSREELEHFADLGYAGYWLSF
                     DPETHARHARLIRDAEHRNAPLTVDTQPLPARAVTEVTVYCADYAGLFSKISGALAVA
                     GASIVDARIHTMTNGMALDTFWVQDTAGGAFDAPHRLARLSVLIEQALSGRLRLSTEI
                     RRVSKILLGRRMQAIHVPPRVVIDNRASNTHTVLEVNGRDRPGLLHDVTAAISEQGLQ
                     IASAHVTTYGVRAVDVFYVKDVFGLKVENERKLRPLRVALLEALNNPDDVTPPITVPP
                     VRKLKRAAAG"
BASE COUNT          623 a          982 c          792 g          471 t
ORIGIN      
        1 ggagcgtttc acgctcactg gaaaggtgcc ggccagttcg ccgcctggcg cctatgcgtc
       61 atggccgtgc ttggcgcggc cgcccacgac ttcctttgca gccgcgagag aaccctgttt
      121 cagtcacagt gaaaccactt tagaccgtgt tcgaaccgat catctggcac caaacccagg
      181 ccccggagca tgatcacgct cgacaaaagg ttggaggcgg gtgaaccacg cgatcagcca
      241 aggttcaaca ttcgcttgaa atgcggattc acgaaaagcg tcccatgcgc ctcggcaggt
      301 cgcgccgatg gatcctctgc accccgccgc tcagcggccc aaaaccaaag gtcccgatca
      361 tcgacagcga gccaggattt tctttccgtg ccctccgcgc tcctcggtga actccgtgtt
      421 gaaagccgcg gccaaccgcc gcacagtcct cgaagctgaa cccgccgccg accaatccga
      481 caacacggcc ggctgacatc aaacccagcg cgaaatccat ccgggcatcc agacgcactt
      541 catcccgcgc acctccagga gtccgtacag cgcagcctga atgccctacg tcaatgctgg
      601 gaaaacaacc gcgtgggacg aagaggtccg tcatggtcca cggtcgagcc acaggcagcc
      661 acccgaagaa agggcgccgg atcgaccgaa cggccatcga cgatcatccc gaaatacaaa
      721 tgcggcccgt aggtaaggcc actgcgtccg acggttgcga tcctttcccc gccatagatc
      781 gtcttgccgc cctccgccag aagcggggcg ataaggccaa gatggctata aaccccaatg
      841 aacccctcat gctgtatcag cacttccaaa ccacctacgc cgtgccgctg aacccgaata
      901 acccttcccg gcgccaccgc cgacacagta ctgccgacag gcgcaggaag gtcgatgcca
      961 tcatgaaacg taccggccag cggccgattc accagaaccc gaggcccgaa tggcgatgac
     1021 acgcaggccg aagcgacagg cggaacaagg gttactgttt cacgatgata aggactcgct
     1081 ccgaccggcg taaaaggaac cgaaccgagc actgcggcaa atagccttat catccatcgc
     1141 cttgatggac gacaacctcg caacctcgtc aactcccgcc accctctgtc agtttcaacc
     1201 gacgaagcgg acaagagcac ggtggatttg tcgaatcagc attctgtgcg atagccgcag
     1261 gcaaaccgct gcgcacctaa cccccctgcg cacctaaccc cgctgcgcac cttaccccgc
     1321 tgcggctctc ttcaacttgc gaacaggggg aacagtgatc ggcggggtca cgtcgtcggg
     1381 attgttcaag gcctccagca aggccactcg aagcggtcgc agctttcgct cgttttccac
     1441 cttcaggccg aagacatcct tcacgtagaa tacgtcaacg gctcgaaccc cataggtggt
     1501 cacatgcgca gaggcgatct gcagtccctg ttcgctgatc gccgccgtca cgtcatgcaa
     1561 caggcccggc cggtcccggc cgttgacctc cagcaccgta tgcgtgttgg aagcgcgatt
     1621 gtcgatgacg acgcgcggtg ggacatggat cgcctgcatg cgccggccca gcaggatctt
     1681 cgacacgcgc cgaatttccg tggacaaccg caagcggccc gaaagcgcct gctcgatcag
     1741 gaccgacagc cgagccagac gatgcggggc atcgaacgcc ccgcccgccg tgtcctggac
     1801 ccagaacgtg tccagagcca ttccattggt catcgtgtgg atccgagcat cgacgatcga
     1861 tgcacctgca accgcaaggg cccctgagat cttgctgaaa agcccggcat agtccgcgca
     1921 atagactgtc acctcagtaa ccgcccgcgc cggcaacggc tgggtatcga ccgtcaacgg
     1981 ggcgttccga tgctcggcgt cgcgaatcag cctggcatga cgggcatggg tttccggatc
     2041 gaacgacagc cagtagcccg cgtaacccag atcggcgaag tgctcgagtt cctcgcggga
     2101 ccagtcggtg agcaacccac caaccgcctg cttcgcccgc gcgacgcgca catcacgttc
     2161 gggcgtggcg agaccccctg ccaggacttc cgcgacgcgc gaataaagct cgcgcagcaa
     2221 cgtcgctttc caaccgttcc agaccttgct tgaaaccgca cgcatgtccg ccacggtcag
     2281 gatcaacaac agccgaagcc gctccggcga ctgaatcgta tccgccagat ccaggatcgt
     2341 cttcggatcg tcgatatcgc gcttgaacgc cgtcgcgctc agaagcaggt ggtgcaggac
     2401 cagccaggag acagtttccg tttcttcatc ggtcaaaccc agggaagggc cgacatccaa
     2461 tgcgacttcg gcgccaagtg cggagtgatc gccgccccgc cctttggcga tgtcgtgcag
     2521 aagcatcgcg aggtaaagcg aacgccgcga ctggacatgg tccacaagct cggacgcgat
     2581 cggcgccacc tccgcgagtt ccccccgctc cagcgtgttg aggacacgca ccgcctcgat
     2641 cgtatgctcg tccacagtga atatatggta cgtgtcgaac tgcatctggc cgacgatgcg
     2701 tgcccagtcc ggtaaatacc gtcctaaaaa cccggtctca ttcaagatac tcagccactt
     2761 ggcgccgtcc ggatgctgcc cgcccgggcg atgacgatcg ggctccttgc cgcacagcaa
     2821 atccataaag accgcggcgg cggcggcatc gccgcgcaac gcaactgc
//
LOCUS       DASWQN010001200         3583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_512538, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001200 DASWQN010000000
VERSION     DASWQN010001200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512538"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2426)
                     /locus_tag="VGC82_20370"
     CDS             complement(<1..2426)
                     /locus_tag="VGC82_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY0425683.1"
                     /translation="MLGSASGFAARVKAVLGPTNTGKTHLAIERLLAHSSGIIGFPLR
                     LLARENYDRMVAIKGARYVALITGEEKIIPPDARWFSCTVEAMPLDRHTEFVAVDEIQ
                     LCADPDRGHVFTDRLLHARGMVETMFLGAETIAPLIRQLVPSAQIETRPRLSQLSYAG
                     PAKLVRLPPRTAVVAFSAAEIYALAEMIRRRKGGCAVVMGRLSPRTRNAQVALYQERE
                     VDFLVATDAIGMGLNMNVDHVAFAGLGKFDGHRPRRLTASEVAQIAGRAGRGMKDGTF
                     GTTTECPPIPEDVVAAVEAHHFESLEMLCWRNSDLDFSSLEGLIDSLNAPPPRSALVR
                     GNEASDLETLTTLSRDPDIRKLAIGRRRMRLLWDACQIPDFRKLADDTHARLCARVFG
                     HVATDTTLPTDWLAAQIAGLDRIEGDIDTLMQRLSGVRVWSYIAARSDWVKDTAHWQA
                     QAREVEDKLSDALHESLTSRFVDRRSAHLVRRLEEAEGRELLSAVTRRGEVVVEGHDV
                     GHIEGFGFVPDPLAVGDEKKLVLRAARRALRAEMPRRVGILESAATDAFALTVDHRVN
                     WDGVKVARLMPGTTSLRPRVQVLDSEFLDGAERERVRARLQSWVDDQVRTDLGPLFAA
                     ETLAQSSPILRGPLHRLMESLGLIPGVDEETLSPDLRRRLREIGVRSGRFALFMPLLM
                     KPRAAAMRVRLWAVRQGIPMPALPNAALVSVPAHQPTWPGGFAAMAGWVEAGPILLRL
                     DIAEKVAGELAFRSRKGPAALPSGLASRFAIKPDMLPAVLRQLGFRVLPAAGLGADQQ
                     GPPAPPMLMP"
     gene            2772..3035
                     /locus_tag="VGC82_20375"
     CDS             2772..3035
                     /locus_tag="VGC82_20375"
                     /inference="COORDINATES: protein motif:HMM:NF013853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 family protein"
                     /protein_id="HEY0425684.1"
                     /translation="MGRSEVDSVESFLTLMMVHLLKLRVWPDSDACNRWRGEIVGFQQ
                     GANKHFTAFMRQKIDIDDPYSWHQAIEGRGAQHRLPGREPVHA"
     gene            3133..>3583
                     /locus_tag="VGC82_20380"
     CDS             3133..>3583
                     /locus_tag="VGC82_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY0425685.1"
                     /translation="MNDTTTPTPEAGPRYDFAAAEPKWQQAWAERRCFRVEDVPTDGK
                     PKYYVLEMFPYPSGKIHMGHVRNYTLGDVVARYKRARGHSVMHPMGWDAFGLPAENAA
                     RERGVHPAKWTYENIAIMRSEFQRMGLSLEWEREFATCEPEYYRHQQK"
BASE COUNT          678 a         1148 c         1141 g          616 t
ORIGIN      
        1 aacggcatca gcattggcgg tgccggtggc ccctgctgat cggcgcccaa accagcggcg
       61 ggaagaacgc ggaaacccag ttgccgcagc actgccggaa gcatatccgg cttgatcgcg
      121 aagcgagagg ccaagccgga tggcaatgcc gccggtccct tgcgcgaccg gaaggccaat
      181 tcgccggcaa ctttctcggc aatatccagc cgcagcagaa tcgggccggc ttcgacccag
      241 cctgccatgg cggcgaaacc gccgggccag gtgggttggt gcgccggcac ggacaccagg
      301 gccgcgttcg gtagtgcggg catcggaata ccctggcgca cagcccatag ccggaccctc
      361 atcgcggccg cgcggggttt catcaggagc ggcatgaaca acgcgaaacg accggagcgg
      421 acgccgattt cacgcagccg ccgacgtaga tcgggcgaca gggtttcctc atcgacgcca
      481 ggaataaggc ccagcgactc catcagccgg tgcagcggcc cgcgcagaat tggactgctc
      541 tgcgccaagg tttcggccgc aaacagcgga cccaggtcgg tccggacttg gtcgtccacc
      601 caggattgca gcctggcccg gacccgttcc cgctccgcgc catcaaggaa ttcgctgtcc
      661 agcacctgca cccggggccg cagcgaggtc gtgcctggca tcaacctggc caccttcacg
      721 ccatcccagt taacgcggtg gtccacggtc aaggcgaatg catcggtggc ggctgattcc
      781 agaatcccga cgcggcgcgg catttccgcc cgcagcgccc gtctggccgc gcgcagcacc
      841 agtttcttct cgtcgccgac ggcaagggga tcgggcacga agccgaagcc ctcgatatgg
      901 ccgacatcgt ggccttcgac gacgacctcg ccgcgccttg ttacggcaga cagcagttcc
      961 ctgccctcgg cttcctccaa ccggcgcacc agatgggccg aacggcggtc cacgaaccgg
     1021 ctggtgaggc tttcgtgcaa ggcatcggac agcttgtcct cgacttcgcg ggcctgcgcc
     1081 tgccagtggg cggtatcctt cacccagtcc gagcgggcgg cgatatacga ccagacccgg
     1141 acgccagaca gccgctgcat cagggtgtcg atgtcgcctt cgatccgatc cagcccggcg
     1201 atctgcgccg ccagccagtc ggtcggtaat gtggtgtcgg tcgcgacgtg gccgaacacc
     1261 ctggcacaaa gacgcgcatg ggtatcatcg gccagcttgc ggaaatcggg gatctggcac
     1321 gcgtcccaca gcagccgcat ccgccgacgg ccgatcgcca gcttgcggat gtcggggtcg
     1381 cgagacagcg ttgtcagcgt ttccaaatcc gatgcctcgt tgccccggac caatgccgaa
     1441 cgaggcggcg gggcattcag gctgtcgatc aatccttcca gcgaagagaa atccaggtcg
     1501 ctgttccgcc agcacagcat ctccaacgac tcgaaatgat gcgcctcgac cgccgccacc
     1561 acatcttccg ggatcggcgg acattcggtt gttgtgccga acgtgccatc cttcatgccg
     1621 cgcccagccc gtccggcgat ctgggcgacc tcggatgcgg tcagccggcg cggacgatgg
     1681 ccgtcgaatt ttcccagtcc ggcgaaagcc acatgatcga cgttcatgtt caggcccatg
     1741 ccgatggcat cggtggcaac caggaaatcg acctcccgct cctggtacag cgccacctgg
     1801 gcgttgcggg tgcgcggcga caggcgtccc atcaccacgg cacaaccgcc tttgcggcgc
     1861 cttatcattt ccgccagcgc atatatttcg gcggcactga acgcgaccac cgcggtgcgc
     1921 ggcggaaggc ggaccagctt ggctggacct gcatagctca gttgcgacag ccgcgggcgc
     1981 gtttcgatct gtgccgaggg caccagttgg cggatcagcg gcgcgatggt ttcggcgccg
     2041 aggaacatgg tttcaaccat ccctcgggcg tgcagcaggc ggtcggtaaa gacatggccg
     2101 cgatcgggat cggcgcaaag ctggatttca tccaccgcga cgaactcggt gtggcggtcg
     2161 agcggcatcg cttcgacggt gcaggagaac caacgcgcgt cgggcgggat gattttttct
     2221 tcgccggtga tcagcgcgac gtagcgggcg cctttgatcg cgaccatgcg gtcatagttt
     2281 tcccgggcca acaggcgcag cgggaaaccg atgatgccgg atgaatgagc aagcagccgc
     2341 tcaatggcga gatgcgtctt gccggtgttg gtcgggccca ggacggcctt gacgcgtgcg
     2401 gcgaaaccgg aagcagagcc aagcatggga tgaatgtttg gatgctgcga cgcggaatgc
     2461 aagctttgat gttgtgaacg gcggccggcc gggtgaggtt gtgcttcacg agagaatgtt
     2521 atttatcgat cgatcttatt ttgggcgttg ctggatggca tgtcccgggg gatattgcgc
     2581 accatcgagt gacccatggt cgctgacatg acatcgtcgt gtcggtataa gcgtgtccat
     2641 gccggatggt ctctacgaac gtgacgtcct ggcttggtcg cagcaccagg ccgacctgct
     2701 ccgccgcctc ggacgcgggg aacgcgtgaa cgatgtggac tgggccaatc tagccgcgga
     2761 gatcgagggc cttgggtcga tccgaagtgg actcggtcga aagctttctc accctgatga
     2821 tggtgcatct tctcaaactc cgcgtctggc ccgatagcga cgcctgcaat cgttggcgag
     2881 gggagattgt cgggttccaa cagggcgcga acaagcactt taccgccttt atgcggcaga
     2941 agatcgacat cgacgatccg tactcatggc atcaagcgat tgaaggcaga ggcgcccaac
     3001 accgccttcc cggccgagaa cccgttcacg cttgaccatc tgctgtggga agatcggagc
     3061 gtgctgctga accgccttcc gtcctcggca taatacttgc gcgggacgct tctccgcccg
     3121 atacattcgc ttatgaacga tacgaccacg cccaccccgg aagccggccc gcgatacgat
     3181 ttcgccgccg ccgaaccaaa atggcagcaa gcctgggcgg aacgccgctg cttccgcgtc
     3241 gaagacgtac cgacggacgg gaagccgaaa tactacgtgc tggagatgtt tccctatccc
     3301 agcggcaaga tccacatggg gcacgtccgc aactacaccc tcggcgacgt cgtcgctcgg
     3361 tacaagcgcg cgcgcggcca ttcggtcatg cacccgatgg gctgggacgc cttcggactg
     3421 cccgccgaaa acgcggcgcg cgaacgcggc gtgcatccgg cgaaatggac ctatgagaac
     3481 atcgccatca tgcggagcga attccagcgc atgggattgt cgctggaatg ggagcgcgag
     3541 ttcgcgacct gtgagccgga atactaccgc caccagcaga agc
//
LOCUS       DASWQN010001201         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_512670, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001201 DASWQN010000000
VERSION     DASWQN010001201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512670"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VGC82_20385"
     CDS             complement(<1..892)
                     /locus_tag="VGC82_20385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041371609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEY0425686.1"
                     /translation="MQLGTALFLPRDIDANLDRWLEPFLAVTGRSTRRKMAPLYVRGL
                     LGSGGRKSIQPMVERLGLSGHDQLHHFISSPAWDDAPLWRVLAEQVDRQVGGEGAVLV
                     VDDSGLPKKGELSVGVARPYCGELGKVANCQVLVSLTLARGEVPLPVGLRLFLPPTWT
                     DDPEHCDAAGVPEAARTAQTKPEIALAEIDRVREAGLRFSCVLADAGFGSSPVFRHGL
                     DERELAWAAGIAYTQLVYPNTVRLHPTHTPTGRVAKHPLPNRPPRAAAKMLATQRWHR
                     ITWRNATKGPLNARFTAMRVR"
     gene            1254..>2210
                     /locus_tag="VGC82_20390"
     CDS             1254..>2210
                     /locus_tag="VGC82_20390"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY0425687.1"
                     /translation="MADNQRWTRRPPGSTWGDWGPDDERGRLNLLTPEKVLQGVAEVK
                     IGKTFCLSLPLDYPGGAILSPRRHPPVLKPTMRGDKPNMNYPLRCDDPTATDIVCDDQ
                     VVLTLQYSTQWDSLAHVGQMFDADGDGKAEDVFYNGYRAGRDIIGPAYYDEKGNMTPC
                     DQPQGARHLGVQNMAESCMQGRGVMIDLEANFGRSGRRVSYEDLMEVMRKDRVVVEPG
                     DFVLFRTGFDQMLLDMGKQPDREVLFSTSCALDGRDARLQQWVSDSGVVALISDHFAV
                     EATPASPCLDDFCATLPLHAHCLFKLGCYLGEMFYLTELADWL"
BASE COUNT          395 a          715 c          706 g          394 t
ORIGIN      
        1 cgcgcacccg catcgcggtg aagcgggcgt tgagcgggcc cttggtggcg ttgcgccagg
       61 tgatgcggtg ccagcgctgc gtggccagca tcttagccgc cgcgcgcggc ggtcggttgg
      121 gcaaagggtg tttggccacg cgcccggttg gcgtgtgggt cggatgcagc ctgaccgtgt
      181 tcggatagac cagctgagta taggcgatac cggctgccca agccagttca cgttcatcga
      241 gcccatggcg gaagacgggg ctgctgccga acccagcatc agccagcacg caggaaaagc
      301 gcagaccggc ttcccgcacc cggtcgatct cggccagcgc gatctcgggc ttggtctgcg
      361 ccgtgcgcgc cgcctccggc acgccggccg cgtcacagtg ctctggatcg tccgtccagg
      421 tgggcggcag gaacagacgc agcccgaccg gcaacggcac ctcgccacgc gccagcgtca
      481 gcgaaaccag cacctgacag ttcgccacct tgcccagctc gccgcagtat ggccgcgcca
      541 cgcccaccga gagttcgccc ttcttgggaa ggcctgagtc atcgaccacc agcaccgctc
      601 cctcgccgcc gacctgcctg tcgacctgtt cagccagcac gcgccacaac ggcgcgtcat
      661 cccaggccgg gctgctgatg aaatggtgca actggtcatg cccggataag ccgagccgct
      721 cgaccatcgg ctggatgctc ttgcgaccgc ctgatcccag cagcccacgc acatacagcg
      781 gcgccatctt gcgccgcgtg ctgcggccgg tcacggcgag gaagggttca agccagcggt
      841 cgaggttagc gtcgatgtcg cgcggcagga aaagagcagt tcccagttgc atgcttgaga
      901 gatgggatcg cacatacaga ccttgaagtg ccaaggtagt gctagacgcc tcggtcgttc
      961 cctgagcttg agaatttcat gttgatcact cacctcaccg tcccagccac ctgggatttt
     1021 cgggcgatga tgcggcaagc ccatcgcgtt gttgctgaca tacaacgcgc gtgcttgcag
     1081 cgagattgcc ttctgcaggg tcggtcggca ttaatgccga tagccttgct cgcatcatcc
     1141 ctcgatgttg ctgccacgga caaaagtccc gctccccggc ctcgtcgaac ccgctctcga
     1201 cacgcgcctg cggccgaagc agggtttgag ccagccgaag gaggaaaccg ctcatggcag
     1261 acaatcaacg ttggacccgc cgcccgcctg gttccacctg gggcgattgg ggaccagatg
     1321 atgagcgtgg ccggctgaat ctgctgacgc cggaaaaggt cctgcaaggt gttgccgagg
     1381 tgaaaatcgg caagacgttt tgcctgtcgc tgccgctgga ttatcccggc ggggcgatcc
     1441 tgtcgccgcg tcgccacccg ccggtgctga agcccaccat gcgcggtgac aagcccaaca
     1501 tgaactatcc gctgcgctgc gatgatccga ccgccaccga catcgtgtgc gacgaccagg
     1561 tcgtcctgac cctgcagtac tccactcaat gggacagcct ggcccatgtc gggcagatgt
     1621 tcgatgccga tggcgacgga aaggcggagg atgtgttcta caacggctat cgcgccggcc
     1681 gcgacatcat cggtcccgcc tactacgacg aaaaaggtaa catgacccca tgcgaccagc
     1741 cgcagggagc gcgtcatttg ggtgtgcaga acatggctga atcctgcatg cagggccgcg
     1801 gcgtgatgat cgatctggaa gccaattttg gccgctccgg acgccgggtc agctacgagg
     1861 acctgatgga ggtcatgcgc aaggacaggg tcgtggtgga gcctggggac ttcgtgctgt
     1921 tccgcaccgg cttcgaccag atgctgttgg acatgggcaa gcagccggac cgggaggtgc
     1981 tgttcagcac gtcttgtgcc ctcgatgggc gcgatgcgag gttgcagcaa tgggtcagcg
     2041 acagcggtgt cgttgcgctg atctccgatc acttcgcggt cgaggcaact ccggccagcc
     2101 cttgcctgga cgacttctgc gccacgctgc cgctgcacgc gcactgcctg ttcaagctgg
     2161 gctgctatct cggcgagatg ttctacctga ccgaactcgc cgactggctg
//
LOCUS       DASWQN010001202         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1305694, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001202 DASWQN010000000
VERSION     DASWQN010001202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305694"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VGC82_20395"
     CDS             complement(<1..419)
                     /locus_tag="VGC82_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425688.1"
                     /translation="MSEAGTTAAEPMPPDPLRSRRWVLALPVAWLMLLLAAPVAVVLL
                     IAVAQPAEGVPPYDVGFNLDNLWLVASDPLYRDALSRSLRVASVSTLICLVVGFPMAL
                     AIARADSRWQNPLLLAVMLPFWTGFLMRINAWIGLLS"
     gene            complement(416..1441)
                     /locus_tag="VGC82_20400"
     CDS             complement(416..1441)
                     /locus_tag="VGC82_20400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425689.1"
                     /translation="MRYGATVALDRLDMAVGRDELFVLLGGSGSGKTTLLRAIAGFVR
                     PDAGRIVLGGRDLSGLPPHRRPVNTMFQSYALFPHMSVEANIGFGLRQQGVSRADTHR
                     RVESMLALVRLEGFGPRRPNQLSGGQQQRVALARSLAPGPGLLLLDEPLSALDQALRR
                     DTRTELVRLRRTLGISFVLVTHDQDEALEMADRIGVMREGRMAQVGTPAELYEQPANR
                     FVAAFLGAANILPCVVDGNAVALPRLGVVVRASRPGTPGPGLLALRAERLRIGGEASG
                     NAVEGTVTGSAYAGDALAVTVRLADGSELRVKRPLSDGLGAARIEPGTMVRVCWQPEA
                     CMLLPSE"
     gene            complement(1594..>1869)
                     /locus_tag="VGC82_20405"
     CDS             complement(1594..>1869)
                     /locus_tag="VGC82_20405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HEY0425690.1"
                     /translation="LAIPSDSRHKTEALEFINFVLRPDIMARITNATHYPNAVPATRV
                     LVRRELLDDPNVFPTDALMQRFFTIGPSTQAADRDRTRLWAKFKAGN"
BASE COUNT          368 a          668 c          560 g          273 t
ORIGIN      
        1 tcgctcagca agccgatcca cgcgttgatc cgcatcagaa accccgtcca gaacggcagc
       61 atcaccgcca acaacagtgg gttctgccaa cgcgagtcgg cccgggcgat cgccagcgcc
      121 atgggaaatc ccaccaccag acatatcagc gtggagacgc tggcgacccg cagactccgc
      181 gacaaggcat cgcgatacag cgggtcgctc gccaccagcc aaaggttatc caggttgaaa
      241 cccacatcat acggcggaac gccctccgcg ggctgggcaa ccgcgatcag caggacgacc
      301 gccaccggcg cagccaaaag cagcatcagc catgcgacgg gcagtgcgag tacccaccgc
      361 cgcgaacgaa gcgggtccgg cggcatcggt tcggccgccg tcgtaccagc ctcgctcatt
      421 cggacggcag cagcatgcag gcctcgggct gccagcaaac ccgcaccatc gttccgggtt
      481 cgatcctggc cgcgcccaat ccatccgaca atgggcgctt gacccgcagt tcggatccat
      541 ccgccaatcg gacggtcacg gccaaagcgt cgccggcata ggcgctcccg gttaccgtac
      601 cctccacggc gttgcccgag gcctccccgc caattcgcag ccgttcggct cgtagcgcca
      661 acaatccagg gcccggggtg cccggccgcg aggcgcgcac gacaacgccc agccgcggca
      721 gcgcgacagc gtttccatcc acgacacagg gcaagatgtt cgccgcgcca agaaacgccg
      781 cgacgaagcg atttgccggc tgttcataca attccgccgg ggtgccgacc tgggccattc
      841 ggccctcgcg catcaccccg atgcggtccg ccatttccag tgcctcatcc tggtcatgcg
      901 tcaccagaac aaaactgatt cccagcgtgc gtctcagccg caccagttcg gttcttgtat
      961 cccgccgcaa tgcctggtcc aacgccgaca gcggctcgtc cagcaacagc agcccgggtc
     1021 ccggtgccag gctccgggcc aaggccacgc gctgctgctg cccacccgac aactggttgg
     1081 gccggcgagg tccgaagcct tcgagacgca ccagcgccag catggactcc acgcgccggt
     1141 gcgtatccgc gcgggagacg ccttgctgcc gcaacccaaa gccgatgttc gcctcgaccg
     1201 acatatgcgg gaacagcgcg taggactgga acatcgtgtt caccggccgc cgatggggcg
     1261 gcaggccgga caggtccctg ccgcccagga cgatgcgccc ggcgtcgggc cgtacgaacc
     1321 ccgcaatggc acgcagcagg gtggtctttc cggaccccga accgccgagc aggacgaaca
     1381 attcgtcgcg cccaaccgcc atgtccagcc ggtccagtgc gacggtcgcg ccgtaacgca
     1441 cagtcaggct ctggacggat aacccagagg tatccgaccc agaggtattc gaccccgggc
     1501 cggtcaaccc agaagtattc aaccccgggc tggtcagcaa gatcgttcac cgccggcctg
     1561 ccgggtcatg ttgaaaagga cccaaacatc cggtcagttt cctgccttga acttcgccca
     1621 aagccgggtg cggtcccgat cggcggcttg cgtcgagggg ccgatggtga agaaccgctg
     1681 cataagagca tccgtcggaa acacgttcgg atcgtccaac agctcccggc gaaccagtac
     1741 ccgcgtcgcc ggcacggcgt tcggataatg tgtcgcattg gtgatccgcg ccatgatgtc
     1801 cggccggagc acgaagttga taaattccaa tgcctcggtc ttgtggcggg agtcggacgg
     1861 gatcgccag
//
LOCUS       DASWQN010001203         4081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_645166, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001203 DASWQN010000000
VERSION     DASWQN010001203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645166"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..890)
                     /gene="yihA"
                     /locus_tag="VGC82_20410"
     CDS             complement(255..890)
                     /gene="yihA"
                     /locus_tag="VGC82_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY0425691.1"
                     /translation="MTPEQAAAALEAGRLLFAAECRFFFGAQKLDQLPPLSGPEIAFA
                     GRSNVGKSSLLNALTGRNSLARTSSEPGRTRQLNFFSLADRLTLVDMPGYGYARASKD
                     IKADWQGMMFDYLRGRPTLLRVMLLMDARIEFKQADHDVMELLDRAAVTFQIVLTKAD
                     SVKPAALARKMAATETAARTHPAAFPRIVCTSSETGQGIPALRAELASLAG"
     gene            complement(1059..2792)
                     /gene="yidC"
                     /locus_tag="VGC82_20415"
     CDS             complement(1059..2792)
                     /gene="yidC"
                     /locus_tag="VGC82_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY0425692.1"
                     /translation="MDQKRLFLAIAVSLAILLGFQMLVAPHLPKPPPQQIAQKTEHSA
                     ATPLEGAPGAAVPPAPLQEPRTVPRLKIEGTRVRGSIDLLGAKLDDLVLKDYREQIDP
                     NSPLVRLLEPRSEDHPYYIQYGWTAPAGETVKLPTNETIWTASPGDLAPGTPVTLSWD
                     NGAGLTFQIGLSIDNEYMFAVNQIVRNATGEAVKLFPWSRIRRDYKPEVAGYYVLFEG
                     LLGVVNGKLQETSYDNAKSEGDKKGGLAFEATSTGGWAGITDKYWLTSLIPDQDIASK
                     VNFRHIDDHGDHYQVDTISVDPLTAPANGEATTVGHVFAGAKVVTLLDRYQAQFHIPS
                     FDKAVDFGWFYFLTKPIFFALDFFNGLFGNFGVAIMVFTVLVKALFFPLANYSYRSMS
                     KMKLLGPKMTALRERLKDDPQKMQQEMMGLYRAEKVNPASGCLPMVVQIPVFFSLYKV
                     IFVTIEMRQAPFFGWIHDLSQVDPTNVFNLFGLIPFDPAAYVPLLHMGLWPLIMGLTM
                     FLQQRLNPPPPDPVQAKLFQFMPIIFTFMMAKFPAGLIIYWSWNNTLSIGQQWLIQRR
                     TRLAKPSLART"
     gene            complement(2792..3034)
                     /gene="yidD"
                     /locus_tag="VGC82_20420"
     CDS             complement(2792..3034)
                     /gene="yidD"
                     /locus_tag="VGC82_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY0425693.1"
                     /translation="MTPAAAVGIGAVRVYQWTLRPFIGSHCRFWPSCSEYAVDALRVH
                     GAARGTAMAAKRILRCNPWHEGGVDPVPGVDRNMGH"
     gene            complement(3132..3530)
                     /gene="rnpA"
                     /locus_tag="VGC82_20425"
     CDS             complement(3132..3530)
                     /gene="rnpA"
                     /locus_tag="VGC82_20425"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY0425694.1"
                     /translation="MPAATDSFSAVKPGSAAKPGRLKRRAEFLRVAASGRKAAVGGVV
                     LQAMRRTDTQPVRLGFTVTKKVGNAVIRNRTRRRLKEAARLLLSQPALLSQQAVAGFD
                     LVLIGRDSTRKRNFILLQGDIRRALERTGV"
     gene            complement(3555..3689)
                     /gene="rpmH"
                     /locus_tag="VGC82_20430"
     CDS             complement(3555..3689)
                     /gene="rpmH"
                     /locus_tag="VGC82_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY0425695.1"
                     /translation="MKRTYQPSKLVRKRRHGFRARMETVGGRRVLANRRAKGRKKLSA
                     "
     gene            complement(3816..>4081)
                     /locus_tag="VGC82_20435"
     CDS             complement(3816..>4081)
                     /locus_tag="VGC82_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019087984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0425696.1"
                     /translation="PVLLMIGDKDTTAIGKNLAPPAIRPTLGNYPVLGKEAAAQIPNA
                     KLVEFPDLGHAPQLQDPEAFHRALLDGLRRPTGSVGDIGSDGD"
BASE COUNT          736 a         1292 c         1264 g          789 t
ORIGIN      
        1 cgagcaggag gatgctttca ttgacctcat cgacctcctg gaccgggggc gtgttgcgtt
       61 tggccatgat tgcttccctt tcgatgggct gcgactgctc gcttcgccac gcaagcggtc
      121 ctgagcatgg accatctccg gattttgcgc gatagcggcg agaaacatcg gcggttcggc
      181 aagcgtccac cgcgttttcc ggccttcatg aagcgcccga ttacgccttc atgacatgcc
      241 ccactacaac gcggtcaacc cgccaacgac gctaattctg cccttagcgc gggaataccc
      301 tggccggttt cgctgctggt gcaaactatg cgtgggaaag cggcggggtg ggtgcgagcg
      361 gcggtttcgg ttgctgccat cttccgtgcc agagcggccg gctttacgct atcagccttg
      421 gtcagcacga tctggaacgt gaccgcggcg cgatccagta gctccatcac gtcgtggtcg
      481 gcctgcttga actcgattcg cgcgtccatc agcagcatca cgcgaagcag cgtcgggcgg
      541 ccacgaaggt agtcgaacat catgccctgc caatcggcct tgatgtcctt ggaggctcgg
      601 gcgtagccgt acccgggcat gtcaaccaag gtcagccggt cggccagact gaagaaattg
      661 agctggcggg tccgtccggg ttcggacgac gtgcgggcga gcgaattccg cccggtcagc
      721 gcgttcagca gcgaagactt gccgacgttg gagcggcccg cgaaggcgat ctccggcccg
      781 gaaagtggtg gcaactggtc cagcttctgg gcgccaaaaa agaaccggca ttcggccgcg
      841 aacagcagac gtccagcctc cagggcagcc gccgcctgct ccggcgtcat gtcgccaagc
      901 gttccactcc cggtgggctc ggaattgggc gacagttcgc ccgaccttga tgtccgttcg
      961 ccggtaacgg cttcgggtcg agcgtgcttc ggtagatccg tcatgacggg gacggccgga
     1021 tcgtgcacaa aacggagcag tcgctcacgg tctaggcctc aggttcgcgc cagcgacggc
     1081 ttggccaggc gcgtgcgccg ctggatcagc cattgttggc cgatcgacag cgtgttgttc
     1141 cagctccagt agatgatcag gccggccggg aacttggcca tcatgaaggt gaagatgatg
     1201 ggcatgaact ggaacagctt ggcttgcacc ggatcgggcg gcggcggatt taacctttgt
     1261 tgcaagaaca tcgtcaggcc catgatcagc ggccacaatc ccatatgcag cagcgggaca
     1321 taggccgcgg gatcgaacgg gatcaatccg aacaggttga acacattggt cggatcgacc
     1381 tgcgacaggt cgtggatcca accgaagaac ggcgcctgcc gcatctcgat ggtcacgaag
     1441 atcactttgt acaacgagaa gaacaccggg atctgcacca ccatcggcaa gcagcccgat
     1501 gccgggttca ccttttccgc gcggtacagc cccatcatct cctgctgcat cttctgcggg
     1561 tcgtccttca gccgctcccg cagcgccgtc atcttcgggc cgagcagctt catcttgctc
     1621 atggagcgat aggagtagtt cgccagaggg aagaacaggg ccttcaccag cacggtgaac
     1681 accatgatcg cgacaccgaa attgccgaac agtccgttga agaagtccag cgcgaaaaag
     1741 atcggcttcg tcaggaaata gaaccagccg aaatcgaccg ccttgtcgaa gctggggatg
     1801 tggaactgcg cctggtagcg gtccagcagg gtcacgacct tcgctccggc gaagacgtgg
     1861 cccaccgtcg tcgcttcccc attggccgga gcggtcagcg gatcgaccga aatcgtatcc
     1921 acttggtagt ggtccccgtg gtcgtcgatg tggcgaaagt taaccttcga agcgatatcc
     1981 tggtccggta tcagcgaggt cagccagtat ttgtcggtaa tgcccgccca gccaccggtg
     2041 ctggtggctt cgaaggccag gccgcccttc ttgtcgccct cggatttggc gttgtcgtag
     2101 gaggtttcct gcaacttgcc gttgacgaca cccagcaagc cttcgaacag cacataataa
     2161 cccgccacct ccggcttgta gtcgcgccga atgcgggacc acgggaacag ctttaccgcc
     2221 tcgccggtgg cgttcctgac aatctggttg accgcgaaca tgtattcgtt gtcgattgac
     2281 agaccgatct ggaacgtcag gccggcgcca ttgtcccacg acaacgtgac cggcgtgccc
     2341 ggcgcaaggt ctcccggcga ggcggtccag atcgtctcgt tcgtcggcag cttcaccgtt
     2401 tcgccggcgg gggcggtcca accgtattga atatagtagg gatgatcctc cgaacgtggc
     2461 tccagcaggc ggacaagcgg cgagttgggg tcaatttgct cgcggtaatc tttcaggacc
     2521 aggtcgtcca gtttggcgcc gagcaggtcg atcgaacccc gaacccgcgt gccctcgatc
     2581 ttcaaccgtg gcactgtccg aggctcttgc aatggcgcgg ggggcaccgc ggcgcccggg
     2641 gcgccttcca acggcgtcgc ggccgagtgc tcggttttct gcgcgatctg ctgcggcggc
     2701 ggtttcggca aatgcggtgc gaccagcatc tggaagccca gcaaaatcgc cagcgatacc
     2761 gcgatcgcca gaaagagccg tttctggtcc atcaatgccc catattccgg tcaaccccag
     2821 gtacgggatc gactccgcct tcatgccacg ggttgcagcg caagatacgc ttcgccgcca
     2881 ttgccgttcc tcgcgccgcg ccgtggaccc gcagcgcgtc caccgcatat tcgctacagc
     2941 ttggccagaa tcgacaatgc gatccgatga acggacgcaa tgtccactga tacacgcgaa
     3001 ccgcgccgat cccgaccgca gccgccggtg tcataccgac gggcctgaat gttcacccgt
     3061 cgccgggtca actaccgtcg gtatccgatt ggtttggcac gccgccgcac caacctttcg
     3121 ggctgctggt atcatacccc ggttctttcc agcgcgcgcc tgatatcgcc ttgcagcaag
     3181 atgaaattcc gtttgcgtgt gctgtcgcgt ccgatcagca ccagatcgaa gccagccacg
     3241 gcctgctggc tcagcagcgc aggctggctc agtagcaatc gagccgcttc cttcagccgg
     3301 cggcgggtgc gattgcggat cacggcgtta ccgaccttct tggtcacggt gaaaccaagg
     3361 cgaaccggct gtgtatccgt tcggcgcatc gcctgcagga caacgccgcc aaccgcggcc
     3421 ttcctgccgg aggctgccac acgcagaaat tccgcgcgcc gcttcaggcg gccgggctta
     3481 gcggcgcttc cgggcttaac ggcgctgaag gagtccgtcg cggctggcat ggacggcaca
     3541 ccagctttcg ccgctcaggc ggacagcttc ttgcggccct tggcacggcg gttggccaag
     3601 accctgcgac cgccaacggt ctccatgcgg gcgcgaaatc catgcctgcg cttgcggacc
     3661 agcttcgacg gttgataggt gcgcttcacg aatactctcc gaaacctttt ggatcatggc
     3721 cgcgatggcc acttccattt cacgcgtgcc agcgggcgca ccttgattgg cgacccgtgg
     3781 ggaggggcgg cttataggga tgccgtcgtt gtgcgtcaat cgccgtcgct gccgatgtcg
     3841 ccgacgcttc cggttggccg ccgcagaccg tcgagcaacg cccggtgaaa cgcctcgggg
     3901 tcctgcagct gcggggcatg gccgaggtcg gggaattcca ccagcttcgc gttcgggatc
     3961 tgtgccgccg cctccttgcc gaggaccggg tagttcccca gtgtcggcct gatggcaggc
     4021 ggtgccaggt ttttgccgat cgccgtcgtg tccttgtcgc cgatcatcag cagtactggc
     4081 a
//
LOCUS       DASWQN010001204         5010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_382120, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001204 DASWQN010000000
VERSION     DASWQN010001204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382120"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VGC82_20440"
     CDS             <1..572
                     /locus_tag="VGC82_20440"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEY0425697.1"
                     /translation="NEGLVFQVHWNPFQLNPDMPKEGRDRAAYRAWKFGSAEKAQVLD
                     QRITEAAAAAGLAFRTDLMTRTPNTIDAHRLIWFAGQNGAQDAAMEAVFKAYFVEGRD
                     IGEHAVLADCAVAAGLPRQDVLDFLASDLADKEMRAADQAAREAGVSGVPSFFLDGYS
                     LFSGAMPAETIANALRRGREVLRNQSAAA"
     gene            691..1983
                     /locus_tag="VGC82_20445"
     CDS             691..1983
                     /locus_tag="VGC82_20445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY0425698.1"
                     /translation="MTSKHPETLALHAGWRRDSATNSVAVPIYQTTSFQFDSAEHAAN
                     LFALKELGNVYTRIMNPTNDVLEQRLAALEGGVAALALASGQAASAFSVLNLAKAGDN
                     FVSSTDLYGGTWNLFANTMKDQGIEARFVDPNDPEAFARATDDRTRAYYAETLPNPKL
                     IPFPIAEVAAIGRSFGVPLIMDNTAAPLLVRPFDHGAAVVMYSTTKYIGGHGNSIGGL
                     VIDGGNFDWEANPARQPAMNTPDPSYHGAVWSQAAKPLGPIAYILKMRVTLLRDLGAP
                     MAPFNAFLFIQGVETLALRMREHSRNALSVANYLAGRKDVSRVIHPAHHKGIGAERTA
                     KYLTRGQGGLVGFELAGGADAGRKFIDSLKLFYHVANIGDARSLAIHPASTTHSQLTE
                     SEQAATGVTPGYVRLSVGLEHIDDILADLSQALDAARG"
     gene            2002..2712
                     /locus_tag="VGC82_20450"
     CDS             2002..2712
                     /locus_tag="VGC82_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0425699.1"
                     /translation="MNEIQRVALVTGGVNGIGAGIASRLAKDGWRVVVADRQPAGTPV
                     EGGRYFVCDVGDEASVSALINSIAVTEQRLDALICNAGFMIRKPIADLSLAEWSSVLT
                     TNLTSTFLLVRSAETLLRAAKGAVVTIASTRARMSEPDTESYSASKGGLVALTHALAI
                     SLGPDVRVNCISPGWILTKGPEPTEKEHAFHPAGRVGRVEDVAELAAFLVGPVSGFIT
                     GGDFVVDGGVTRKMIYPE"
     gene            2831..3580
                     /locus_tag="VGC82_20455"
     CDS             2831..3580
                     /locus_tag="VGC82_20455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425700.1"
                     /translation="MSETIDREGYRYIPGPFQYSAGVAALPGHAIERVRFANPIPLTE
                     GFKRIETFLNQRGLPLTAFCACELRSPAPFNDAGFIAFNRDYVGTLERWGVVANDRNP
                     VARSNVCPEIDPPATPSFHAFCLVTPSVAAAPSFVCAGSGEAREGAGPYREKTIRYND
                     VTPDAMAEKARFVLAAMEKRMAALGASWASATAVQAYTVHDIYPFLAPEIVRRGAARH
                     GLTLQFCRPPVIGLEYEMDCRGVQTEYVLSE"
     gene            3673..4809
                     /locus_tag="VGC82_20460"
     CDS             3673..4809
                     /locus_tag="VGC82_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0425701.1"
                     /translation="MPDSISATGDTLIIDMIRRFSQERLAPLAAEREKQKRIEPEIIA
                     ELGELGVFGATTPAEWDGSEIDPVTYAMLLEEIAAGDGSVSTMVSVHNSPTCIVVNNY
                     GNAAQKDRWLRKLATGEHIGSFGLTEPQAGSDASNLKTRAVKKGDRYVVNGAKQFISN
                     AAHPGSLVFFAVTDPDAGKKGLSAFVIEKNHPGFSIARTEEKLGQKASDTCALAFDDM
                     EVPEDQRIGAEGEGYKIALSTLESGRIGIAAQSVGMARAALDYAIGYARDRKAFGGAI
                     IDLQAVGFRLADAKTKLEAARQLTLYAARLKADGRPALEAACMAKLFASEAAEAICTA
                     SIQTLGGYGYLADYPVERIYRDVRVCQIYEGTSDVQKLILQRML"
BASE COUNT          951 a         1629 c         1550 g          880 t
ORIGIN      
        1 cgaacgaggg acttgtcttc caggtccact ggaacccgtt tcagctcaat cccgacatgc
       61 cgaaggaagg ccgtgatcgc gctgcctacc gcgcctggaa attcggcagc gccgagaaag
      121 cccaagtgct cgaccagcgc attaccgagg cggcagccgc ggccggctta gccttccgca
      181 cggacctgat gacccgtacg ccgaacacga tcgacgccca ccgcctgatc tggttcgctg
      241 gccagaacgg cgctcaggac gcggcgatgg aggcggtgtt caaagcctac ttcgtcgaag
      301 ggcgcgatat cggcgagcac gctgtcctgg ccgattgcgc tgtcgcagcg gggctgccgc
      361 gccaagacgt gctggatttc ctggcgagcg atctcgcgga taaggaaatg cgagcagcgg
      421 accaagcggc acgcgaagcc ggcgtatccg gcgtgccgtc attcttcctg gacggctaca
      481 gcctattttc cggcgcgatg ccggcggaaa ccatcgccaa tgcgcttcgt cgcggccggg
      541 aggtgctacg aaaccagtcg gcggcggcct gatcttccgt cctcggtcag cggcattgct
      601 ccaccacacg aacgaacagc cacagcttgc tttaccgccc ccgcccgttg cctatagtgc
      661 gccgcgaacc gaaccaggaa ggcaccggtc atgaccagca agcatcccga aacccttgcc
      721 ttgcacgccg ggtggcgccg cgacagcgcc accaattcgg tcgcggtgcc gatctaccag
      781 accacctcgt tccagttcga cagcgctgaa catgccgcga atctgttcgc gctgaaggag
      841 ctggggaacg tttacacccg catcatgaac cccaccaacg atgtgctgga gcagcggctt
      901 gcagcgctcg aaggcggcgt tgcggccctg gccctcgcat ccggccaggc tgcctcggct
      961 ttttcggtgt tgaacctggc gaaggccggc gataatttcg tcagctcgac tgacctttac
     1021 ggcggcacct ggaacctgtt cgccaacacc atgaaggacc agggcatcga agcgcggttt
     1081 gtcgatccga acgaccccga ggcctttgcc cgcgccaccg acgaccgcac ccgcgcttat
     1141 tacgcggaga ccttgccgaa cccgaagctg atcccgttcc cgatcgctga agtcgcggcg
     1201 atcggccggt ccttcggcgt tccgctgatc atggataaca ccgcggcccc cttgctggtg
     1261 cgcccgttcg atcacggagc ggccgtggtg atgtattcga ccaccaagta tatcggcggc
     1321 cacggtaact cgattggtgg cctcgtcatc gacggcggca atttcgactg ggaagccaac
     1381 cctgcacgcc agccggcgat gaacacgccg gacccatcct accacggcgc cgtctggtcc
     1441 caagccgcca agccgctcgg tcctatcgcc tacatcctaa agatgcgggt taccttgttg
     1501 cgcgacctcg gcgcgcccat ggcgccgttc aacgccttcc tgtttatcca gggcgttgaa
     1561 acgctggcgc tgcgaatgcg cgagcacagc cgcaacgcgc tgtccgtcgc caactacctg
     1621 gccggccgca aagacgtctc gcgggttatc catcctgccc accacaaggg gatcggcgcc
     1681 gaacggacgg cgaaatacct gacgcgcggg cagggcggcc ttgtagggtt cgaactggcg
     1741 gggggtgcgg atgccgggcg caagttcatc gactcgctga agctgttcta ccacgtcgcc
     1801 aacatcggcg atgcgcgcag cctggcgatt catccagcca gcaccacaca ctcccagctg
     1861 acggaatcgg agcaggcggc gacgggcgtg acgccgggct atgtgcgact gtcggttggc
     1921 ctggagcata tcgacgacat cctggccgat ctgtcgcagg cgctcgacgc tgccaggggc
     1981 tgagctcgcg ggagccaggg catgaacgag atacagcgcg ttgccctggt caccggcggc
     2041 gtgaacggca ttggcgccgg catcgcgtcc cggcttgcaa aagacggctg gcgggtggtc
     2101 gtcgcggatc gccagcctgc cggcacgccg gtcgagggcg gacgttactt tgtctgcgac
     2161 gtcggcgacg aggcttccgt gtccgcgctg atcaactcga tcgctgtaac cgaacagcgg
     2221 ctggatgcgc tgatctgcaa cgccggattc atgatccgaa agccgatcgc cgacctgtcc
     2281 cttgccgaat ggtcgtccgt actgacgacc aacctgacca gcacattcct tctggtgcga
     2341 agtgccgaga cgttgctgcg tgcggccaaa ggcgcggtcg tcaccatagc ctcgacacgc
     2401 gcgcgtatgt cggaacctga cacggaatcg tactccgcct ccaaaggagg tctggtggca
     2461 ctgacgcacg cattggccat cagcctgggg ccggacgtcc gggtcaattg catcagcccg
     2521 ggttggatcc tgaccaaggg gccggaacca acggagaaag agcacgcgtt ccatcctgcc
     2581 ggccgcgtcg gccgcgttga ggacgtagcg gaactggcag ccttccttgt cgggcccgtg
     2641 agcggcttta taaccggcgg ggatttcgtc gtggacggcg gcgtgaccag gaagatgatc
     2701 tacccggagt agttgcggcg atgatgaatt ggaaaactgg ggccgcatga caatgtctcg
     2761 ataacggccc ttacgataga gttccgagct tgagcggtgc cctgatcggc gtccacgcgg
     2821 gagcacactt atgagcgaaa ccatcgaccg cgagggatac cgatatatcc ctggcccatt
     2881 ccaatactcg gccggcgtgg ctgccctgcc aggtcatgcg atcgagcggg tccgattcgc
     2941 caatccgata ccgctgaccg agggtttcaa gcggatcgag acgtttctga accagcgcgg
     3001 cctgcctctg acggcgtttt gcgcgtgtga acttcgctcc ccggcgccgt tcaacgatgc
     3061 tggcttcatc gccttcaaca gggactatgt cggcacgctg gagcgttggg gagtcgtcgc
     3121 gaacgaccgc aacccggtcg cacgcagcaa tgtctgcccc gagatcgatc ctccggcgac
     3181 accaagtttc catgcgttct gtcttgtcac gccgtccgtg gccgccgcgc catctttcgt
     3241 ctgcgccgga agcggcgaag ctcgggaagg cgctgggcca taccgggaaa agaccattcg
     3301 ttacaacgac gtcacgcctg acgcgatggc ggaaaaggca cgtttcgtgt tggcggcgat
     3361 ggaaaagcgc atggccgccc tcggggcttc gtgggcctcg gccacggcgg tacaggctta
     3421 cacggtgcac gacatctacc cgtttctcgc gccagagatc gtccggcgcg gcgccgctcg
     3481 ccatgggctg accttgcagt tctgccgtcc gccggtgatc gggttggaat acgagatgga
     3541 ttgccgcggc gtacagaccg aatatgtcct gtcggaataa gctgtgccca tgccggcacg
     3601 gcatccaggc tgacaaaccg cccgccgcgg tgtagatcgc ttccaccgtc cgcaaccccc
     3661 gaggctatcg tcatgcccga cagcatttct gccaccggcg acacgctgat catcgacatg
     3721 atccgccgct tttcccagga gcgtctcgcg ccgctggcag ccgaacgcga aaagcagaag
     3781 cgcatcgaac cggagatcat cgcggaactt ggggaacttg gcgtgttcgg cgccaccacg
     3841 ccggccgagt gggacggcag cgaaatcgac ccggtcacct atgcgatgtt gctggaggaa
     3901 atcgccgccg gcgatggatc ggtctccacc atggtttcgg tgcacaactc cccaacctgc
     3961 atcgtcgtca acaactacgg gaatgccgcc cagaaggacc gttggctccg caagttggcg
     4021 accggggagc acatcggctc gtttgggctc accgagccgc aagccgggtc cgacgcgtcc
     4081 aacctgaaga cccgagcagt caagaagggc gaccgctatg tcgttaacgg cgccaagcag
     4141 ttcatcagca acgcagccca tccaggaagc ctggtgtttt tcgctgtcac cgatccggac
     4201 gccggcaaaa agggtttgtc cgctttcgtg atcgaaaaaa atcaccccgg tttctccata
     4261 gccaggaccg aggaaaagct cggccagaag gcctcggaca cctgtgccct ggcgttcgac
     4321 gatatggaag tacccgagga ccagcgcatc ggcgcagagg gcgagggcta caagatcgcg
     4381 ctgtccacgc tggaatccgg ccgcatcggc atcgccgccc aaagcgtcgg catggcccgg
     4441 gccgcactgg actatgcgat cggctatgct cgggaccgca aggccttcgg tggcgcaatc
     4501 atcgacctgc aggcggtcgg cttccgtctc gccgacgcca agaccaagct ggaagcggca
     4561 aggcaactga cgctttatgc cgctcgcctg aaagccgacg gccgaccggc gctggaagcc
     4621 gcctgcatgg ctaaactgtt cgcctccgaa gccgccgagg cgatctgcac tgcctccatt
     4681 caaaccctcg gtggatatgg ctacctcgcc gactatccgg tggagcggat ttatcgcgat
     4741 gtgcgagtat gccagattta cgagggaacc tctgacgtgc agaagctgat cctgcagcgg
     4801 atgctgtaat accaacagcc cctactgatg accgttccat cagtaagggc tgttggtatc
     4861 gcgcgccggg ttggtcggcg gcggcgcgca aaatcaataa gataccggcg gccgctgacc
     4921 cgcctatggg tcaacactta aggccgttgg tatgatgctg accctgaccg atgtccacag
     4981 ctactacggc cgcgcccaca tcctgcaagg
//
LOCUS       DASWQN010001205         4537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1306157, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001205 DASWQN010000000
VERSION     DASWQN010001205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306157"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VGC82_20465"
     CDS             <1..463
                     /locus_tag="VGC82_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEY0425702.1"
                     /translation="LGFLFGLGFDTATEIGVLTMSGLGAAQGLPIWAVLIFPALFTAG
                     MTLIDTTDSILMLGAYGWAFIKPIRKLYYNMTITLVSVVIALVVGGIEALGLIGEKLG
                     LDGGFWGMIAGLNEDFGTLGYLIIGIFIASWALSALIYRVKGYDRIEVTGA"
     gene            complement(540..1733)
                     /locus_tag="VGC82_20470"
     CDS             complement(540..1733)
                     /locus_tag="VGC82_20470"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0425703.1"
                     /translation="MASAKTEGAVPDFLRMDQAISSDLANARKAGNWFGWPTKDLTPV
                     TLADSEGRTVRIRTPVMDLEGLITPTNLHYTVQHFAVPPVVETAAWELQVSGHVKRPL
                     TLNFEQLRRFPGRSVRTVMECSGSDATYFEYLKGEGPKPSRTQEGMILSASEWTGVPL
                     AAVLHEAGLTAKSLYVRAEGNDLGVPPSAAEGTKPFYYDKGLPIEKALHPDTILAWAQ
                     NGQLLEHLHGAPVRLLVPGWSGNWSVKWLNKLELMEREPDCWYHYQFYYYGESADDPD
                     KELITTIGVKSMITHPNDDTETMPPGVHMIRGYAWSGAGAIAHVDVSTDGGENWHSAH
                     IEQPRDRFMWVRWSYRWDASQKGTFSLMSRATDEVGRVQSREPRYNNMRKNFSAIVGY
                     TVTVA"
     gene            1887..2354
                     /locus_tag="VGC82_20475"
     CDS             1887..2354
                     /locus_tag="VGC82_20475"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0425704.1"
                     /translation="MIEINGMAHVILTVGDFAKARAFYCELLPFLGMTKVYDGNNFVY
                     HVGGRTALGIQRCAPPHEGERFVQNRVGLHHLCLRARSREDVDKAAAKLREMGAYIDR
                     GPMEREWAEGYYFFVFEDPDGIRLEINHIPGKGLLTEGGRQNPSDDPNWDQNA"
     gene            2566..>4537
                     /gene="secA"
                     /locus_tag="VGC82_20480"
     CDS             2566..>4537
                     /gene="secA"
                     /locus_tag="VGC82_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043509192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY0425705.1"
                     /translation="MLASIARAIFGSANDRSLKGYQRRVPAINALEDGMKALSDEALS
                     SKTAEFRRRLADGATLDDLLPEAFAVVREASRRTLGQRHFDVQLVGGMVLHDGKISEM
                     KTGEGKTLVATLPVYLNALAGNGVHVVTVNDYLARRDADWMGEIYNFLGLTYGVIVHG
                     QDEETKRAQYAADITYGTNNEFGFDYLRDNMKYRLEDMVQRDFFYGIVDEVDSILIDE
                     ARTPLIISGPAEDSSDLYRQVDLVVKELVKDASTYDKDEKFKTVSLTEPGSEQVEDML
                     KSAGVITEGNLYDIFNVSVVHHVQQSVRAHTLFARDVDYIVRDDQVIIIDEFTGRMMQ
                     GRRYSEGLHQALEAKEHVTVQAENQTLASITFQNYFRLYPKLAGMTGTAMTEADEFAE
                     IYKLDVVEIPTNVAVNRKDEDDEVYRSAAEKYEAVASLIAEARERGQPVLVGTTSIEK
                     SETISDLLKKKKVPHSVLNARFHEQEAEIVSQAGAPGAVTIATNMAGRGTDIKLGGNL
                     DVRLRKELANIHDDPRRATREAQVREEVAIAHQEVKDAGGLFVIGTERHESRRIDNQL
                     RGRSGRQGDPGHSRFFLSLEDDLMRIFGSERMGGMLQRLGLKEGEAIVHPWINKALEK
                     AQKKVEARNFDTRKNVLKYDDVMNAQRKEVYAQ"
BASE COUNT          858 a         1430 c         1418 g          831 t
ORIGIN      
        1 gctcggtttt ctcttcggcc tggggtttga caccgcgacg gaaatcggcg tgctcacgat
       61 gtccggcctg ggcgccgcgc aaggtctgcc gatctgggcg gtgctgatct ttcccgcgct
      121 gttcaccgcc ggcatgaccc tgatcgacac caccgacagc atcctgatgc tgggcgccta
      181 tggctgggcc ttcatcaaac cgatccgcaa actctactac aatatgacca ttaccttggt
      241 gtcggtcgta atcgccctcg tcgtcggcgg catcgaggcg ctcggtctga tcggcgagaa
      301 gctggggctc gacggtgggt tttggggcat gatcgccggc ctgaatgagg attttggcac
      361 cctcggctac cttatcatcg gcatcttcat cgcaagctgg gccctgtcgg cgctgatcta
      421 ccgtgttaaa ggttacgacc ggatcgaggt caccggcgca tagtccttcc gaaggcgcgt
      481 ggatgccgac cttcgtcggc acgacgaggt ggtgccgaca gtgggtcgat ccgcgtaacc
      541 tatgccaccg tgaccgtgta gcccacgatg gcgctgaaat tcttgcgcat gttgttgtag
      601 cgcggctcgc gggactgcac gcggcccacc tcgtccgtag cgcgggacat caggctaaag
      661 gtgccctttt ggctcgcgtc ccagcgatac gaccaccgca cccacatgaa ccggtcgcgc
      721 ggctgctcga tatgggcgga gtgccagttc tccccgccgt ccgtgctgac atcgacgtgg
      781 gcgatggcgc cggcgccgct ccaagcgtag ccccggatca tgtgcacgcc cggcggcatc
      841 gtctcggtgt cgtcgttggg atgggtgatc atggacttca ccccgatggt ggtaatcagc
      901 tccttgtccg ggtcgtccgc cgattcgccg taatagtaga actggtagtg gtaccagcag
      961 tccggctccc gttccatgag ctccagcttg ttgagccatt ttaccgacca gttgcccgac
     1021 cagccaggca ccaacaggcg taccggggcg ccgtggagat gctccagcaa ctggccgttc
     1081 tgcgcccagg ctaggatcgt gtcggggtgc agcgcctttt cgatcggcaa gcccttgtcg
     1141 tagtagaacg gcttggtgcc ctcggcggcg ctgggcggca cgccaaggtc gttgccctcg
     1201 gctcgcacat acagcgattt ggcggtcagg ccggcctcgt gcagcacggc ggccagcggg
     1261 acgccggtcc actcgctggc gctcaggatc atgccttcct gggtgcgcga cggtttgggt
     1321 ccctcgccct tgaggtactc gaaatacgtg gcatcgctgc cggaacactc catcacggtg
     1381 cggacgctac ggccgggaaa gcgccgcaat tgctcgaaat tgagcgtcaa tggtcgcttg
     1441 acgtggccgc tgacctgcag ttcccaggcc gcggtttcca ccaccggcgg caccgcgaaa
     1501 tgctgcaccg tgtagtgcag gttggtcggc gtgatgagcc cctccaggtc catgaccggc
     1561 gtcctgatcc gcaccgtccg gccttcgctg tcggccagcg tgaccggagt cagatccttg
     1621 gtgggccagc cgaaccagtt gccggccttc cgagcgttgg ccaggtccga actgatcgct
     1681 tggtccatgc gcaggaaatc gggaaccgca ccttccgttt tcgccgaggc catgctgctg
     1741 tccttatgct cgccggagct gacgcatggt gagcatgcca atcgggataa atcaatcgat
     1801 cgaccttccg cggcgatgcg tcatcagaac gactgccggg tggcgatgac cgcaggagcc
     1861 tcaacggtgc caaaggaaac tcaacgatga tcgagatcaa cggcatggcc cacgtcatcc
     1921 tgacggtcgg cgacttcgcc aaggcgcgcg cgttctactg cgagctcctg ccgttcctcg
     1981 gcatgacgaa ggtctatgac ggcaacaact tcgtttatca cgtcggcggc cgtaccgcgc
     2041 tcggcatcca gcgctgcgcc ccgccgcacg agggcgagcg ttttgtccag aatcgcgtcg
     2101 gcctgcacca tctgtgtctg cgtgcccggt ctcgcgagga tgtcgataag gccgccgcca
     2161 agctgcgcga aatgggagcg tatatcgacc gcggtccgat ggagcgcgag tgggccgagg
     2221 gctattactt cttcgtcttc gaagatccgg acggcattcg gctcgagatc aaccatatcc
     2281 ccggcaaagg gctgctgacc gaaggcggac ggcagaatcc cagcgacgat cccaattggg
     2341 accagaacgc ttgaaaggtg ttcccgcccg acaaatcgag ctgggtgccg ccgccgaaca
     2401 cggtgccgcg gcgcttgcgg ccgggaacct cgcgaaacgg gataagcgcg ccagccaata
     2461 taaaccgtca aggatttccg ttgtcgttga ccctgcggcg tccgagtcct atctgattgc
     2521 ggcgcgtctg ggtcttcccg gaccggtcgc ccatggaagg tctgcatgct cgcctctatc
     2581 gctcgtgcca ttttcggctc tgccaatgat cgctccctga agggctatca gcggcgcgtt
     2641 ccggcgatca atgccctgga agacggcatg aaagcgctgt ctgacgaagc cttgtcctcc
     2701 aagaccgcgg agttccgccg gcgcctggcc gacggcgcga cccttgacga cctgctgccc
     2761 gaagccttcg ccgtggttcg cgaagccagc cgccgcacgc tcgggcagcg gcatttcgac
     2821 gtgcagctgg tcggcggcat ggtgctgcac gacggcaaga tctcggaaat gaagaccggc
     2881 gaaggcaaga ccctggtagc cacgctgccg gtctatctga acgcgctcgc cggcaacggc
     2941 gtgcatgtgg tgacggtgaa cgactacctc gcccgccgcg acgccgactg gatgggcgaa
     3001 atctacaact tcctcggcct gacctatggc gtcatcgtcc atggccagga tgaggaaacc
     3061 aagcgcgcgc agtatgccgc cgacatcacc tatggcacca acaacgagtt cggcttcgac
     3121 tacttgcgcg acaacatgaa ataccgcctt gaagacatgg tccagcgcga cttcttctac
     3181 ggcatcgttg acgaggtgga ctccatcctg atcgacgagg cccgcacgcc gctgatcatc
     3241 tccggcccgg ccgaggacag ctccgacctg tatcgccagg tcgatctggt ggtgaaggag
     3301 ctggtgaagg acgcctcgac ctacgacaag gatgaaaagt tcaagacggt ttcgctgacc
     3361 gaacccggca gcgagcaggt cgaggacatg ctgaaatccg ccggcgtgat caccgaaggc
     3421 aatctgtacg acatcttcaa tgtttccgtc gtccaccacg tccagcaatc ggtccgagcc
     3481 cacacgctgt tcgcccggga tgtcgattac atcgtccgcg acgaccaggt gatcatcatc
     3541 gacgagttca ccggccgcat gatgcagggc cgccgctact cggaaggtct gcaccaggcg
     3601 ctggaggcca aggagcacgt caccgtccag gcggaaaacc agaccctggc ctcgatcacc
     3661 ttccagaact atttccggtt gtatcccaag ctggccggca tgaccggcac ggcgatgacc
     3721 gaagccgacg aattcgccga gatctacaag ctggatgtgg tcgagatccc caccaacgtt
     3781 gccgtcaacc gcaaggacga ggacgacgag gtttatcgct ccgccgccga gaaatacgag
     3841 gcggtcgcat cgctgattgc cgaggcccgg gaacggggcc aaccggtcct ggtcggcacc
     3901 acttcgatcg aaaagagcga gacgatcagc gatctgctga agaaaaagaa agtgccgcat
     3961 tcggtgctga acgcgcgttt ccacgagcag gaggccgaga tcgtgtcgca ggcgggtgca
     4021 cctggcgcgg tgaccatcgc caccaacatg gccggccgcg gcaccgacat caaacttggc
     4081 ggcaacctcg atgtgcggct gcgcaaggaa ctcgccaaca ttcacgacga ccccaggcgg
     4141 gccacgcggg aggctcaggt tcgcgaggaa gtcgccatcg cgcatcaaga ggtgaaggac
     4201 gcgggcggtc tgtttgtcat cggcaccgaa cgccatgaaa gccggcggat cgacaaccag
     4261 ttgcgcggtc ggtccgggcg gcagggcgat ccgggccatt cgcgcttttt cctgtcgctg
     4321 gaagacgacc tgatgcggat cttcgggtcc gaacggatgg gtggcatgct gcagcggctg
     4381 ggcctgaagg aaggcgaggc gatcgtccat ccatggatca acaaggcgct ggaaaaagcc
     4441 cagaagaagg tcgaggcgcg gaacttcgac acccgcaaga acgtcctgaa atacgacgac
     4501 gtcatgaatg cccagcgcaa agaggtttat gcccagc
//
LOCUS       DASWQN010001206         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1172784, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001206 DASWQN010000000
VERSION     DASWQN010001206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172784"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VGC82_20485"
     CDS             <1..123
                     /locus_tag="VGC82_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0425706.1"
                     /translation="VERCWSRLKEWRAVATRYEKSERSFMGVLCMVATMDWFKA"
     gene            498..899
                     /locus_tag="VGC82_20490"
     CDS             498..899
                     /locus_tag="VGC82_20490"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425707.1"
                     /translation="MGSAGDIGYGRRRLSLPHRSQHVHDHLRGCEHLPQECEDLPITH
                     PKVADAAVFGVPNADLGEEVKAAVQLMVGCAPGPETEAELIAFCSQHLARQKVPRSID
                     FELELPRLPTGKLYKRVLRDRYWANRTSRII"
     gene            1194..>2224
                     /locus_tag="VGC82_20495"
     CDS             1194..>2224
                     /locus_tag="VGC82_20495"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEY0425708.1"
                     /translation="MVATRSLIRIAVNAALDGGLAALSVPVARWLADPAGPVLHPLWT
                     IPLGAISLLLAGIPFRLSLQYWRFAAIGDLLTLAASAAAGAALFAAVTTLLVGAPPTL
                     AFPVIHALTLLLFLGAPRVGYLWRRGRPALKPAHIDASSILLVGSIEDADLFIRALAQ
                     DRRQAFRVEGLLASSDRQTGRRIQGHRILGSLNDAGTVLQQLQAEDCLPGTLVFVTPE
                     LSGTRLAQVVAQADRFGLQVRQAPRPTALADPDKPRPVELRPVAIEDLLNRPQVPLDR
                     DGMARLIQGRRVIVTGAGGTIGSELARQVAAFGPGTLILLDNGEYALWQIDLELAETH
                     PQVNRRTLIA"
BASE COUNT          378 a          714 c          694 g          438 t
ORIGIN      
        1 gtcgagcgct gctggtccag attgaaagaa tggcgcgccg tcgcgacgcg ctatgagaag
       61 agtgagcgct ccttcatggg cgtgctctgc atggttgcaa ccatggattg gttcaaagca
      121 tagcaaagag ttcaagaact aacaggccct cgtggagagc cactgggtca cccacagcca
      181 gcttgtgccg acgatgttct cgcgcatgct caaggtcaag gagcaaatga tcgcctggtg
      241 gggcccgatc gttcatgaat actatggaat gaccgcaggc atgggcatca ccgcctgcga
      301 tagcaccgag tggctgggcc acagggggta cggtcggccg cgtcgtgttc ggcgatcttc
      361 acttcctgga cgcggcgatg aacccctgcc cgactcgaac gatcggtatg atatggttca
      421 ggcttaggct tgagtcgtca ttcgaatatt ttaacgacaa ggcacggacg gcggaggtcc
      481 tttccgctga tggcaccatg ggcagcgccg gcgacatcgg ctatggacga agacggttat
      541 ctctacctca cagatcgcag cacgttcatg atcatctccg ggggtgtgaa catctcccgc
      601 aggagtgcga ggacctgccc atcacccatc ccaaggtcgc cgatgccgcg gtgtttggcg
      661 tgccgaatgc cgacctgggg gaagaggtca aggcggctgt gcagcttatg gtcggctgtg
      721 cccctggacc ggaaaccgaa gctgaactga tcgccttctg tagtcagcat ctggcgcggc
      781 agaaagtgcc acggtcgatc gattttgagc tcgaactgcc gagattgccg accggcaagc
      841 tgtataaacg cgtgcttcgc gatcggtact gggcgaacag gacaagccgg atcatataga
      901 accaaacgcc ggctgaaggg gtcagatccc gtaaggcagt aacctcggtg gaatcgcggt
      961 ggaaatcgcc catccgacag atgccgggca tcttcggtgc ctcggccggt cgatagtcag
     1021 gcgctgctgc ggatggtgac gaagccgcgg cttggaaagc gggtgcgaag ccgcagggat
     1081 gtaactcaaa aaggtgacat gcaccttttg cattgcttcc ggctcgttga tgatcgaacc
     1141 gttggcgtgt tcccggctat gcccccgact tcgcccgcgc tataccccgc cagatggtcg
     1201 cgacacgttc cctgattcgc atcgccgtca acgccgccct ggatggcggc ctggcggctc
     1261 tatcggtgcc ggtggcgcgc tggctcgccg acccggcggg gcccgtactg catccgctct
     1321 ggactattcc actcggcgcg atcagcctgc tgctcgccgg catcccgttc cggctttccc
     1381 tgcaatactg gcgctttgcc gccattggcg accttctgac actggctgcc agcgccgctg
     1441 ccggggccgc cctgtttgcc gccgtcacaa cgcttctggt cggtgcgccg cctaccctcg
     1501 cgtttcccgt catccatgcc ctgacgttgc tgctgttcct gggcgctccc cgggtcggct
     1561 atctctggcg ccgcggccgt cctgcgctca agccggcgca tatcgatgcg tcctcgatcc
     1621 tactggtcgg cagcatcgaa gacgcggacc tgttcatccg cgccctcgcc caggatcgcc
     1681 gccaggcctt ccgtgtcgag ggcttgctgg cctccagcga tcgccagacc gggcggcgca
     1741 tccagggcca tcgcatcctt ggttccctca acgacgccgg tacggtgttg cagcagttgc
     1801 aggcggaaga ctgccttccc ggtaccctgg ttttcgtcac ccccgagctt tccggcaccc
     1861 gccttgccca agtcgttgct caggcggacc gctttgggct tcaggtgcgc caggcgcctc
     1921 ggcccaccgc gctggccgat ccggacaagc cgcgtccggt cgaactgcgt ccggtcgcga
     1981 tcgaggatct gctgaatcgc ccgcaggtgc cgctcgaccg ggacggaatg gctcggctga
     2041 tccagggccg ccgggtcatc gtcactgggg ctggcggcac gatcggcagc gagcttgccc
     2101 gccaggtcgc cgcattcggc ccaggcacct tgatcctgtt ggacaatggc gaatacgcct
     2161 tgtggcagat cgatctggag ctggcggaaa cccatcctca ggtgaaccgc cgcaccctta
     2221 tcgc
//
LOCUS       DASWQN010001207         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1172898, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001207 DASWQN010000000
VERSION     DASWQN010001207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172898"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VGC82_20500"
     CDS             complement(<1..816)
                     /locus_tag="VGC82_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0425709.1"
                     /translation="MKRISLVVTLALAGGFSTQAATAQTLKTVKDRGMLSCGVSQGLP
                     GFSSPDDKGNWTGLDVDVCRAVAAAIFNDPTKVKFVPLSAKDRFTALQSGEIDVLSRN
                     TTWTLSRDTSLGANFTGVTYYDGQGFLVKKSLKVNSALELNSASVCVQQGTTTEQNLA
                     DYFKGNNMKYEVIAFATNDEAIKAYESGRCDVFTTDVSGLYSDRLKLANAADHAVLPE
                     VISKEPLGPMVRHGDDQWFDIVKWSLYAMITAEELGITQKNVDDMAKSDKPEMK"
     gene            1201..>1524
                     /locus_tag="VGC82_20505"
     CDS             1201..>1524
                     /locus_tag="VGC82_20505"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY0425710.1"
                     /translation="MLVTAGRDTKAQKGFVNPPVVHGSTVLYPTAEDLHAHRSEFQYG
                     RRGTPTTKALQEALMALEGPQCAGVGLAPSGLSAITTTLLAVLKAGDHLLVCDNAYRP
                     SRNFCN"
BASE COUNT          303 a          450 c          464 g          307 t
ORIGIN      
        1 cttcatctcg ggcttgtccg atttagccat gtcgtcgacg ttcttctggg taatgccgag
       61 ttcttccgcc gtgatcatcg cgtaaagcga ccacttcacg atgtcgaacc actggtcgtc
      121 gccatggcgc accatcggac cgagcggctc tttcgagatc acttccggaa gcacggcatg
      181 atcggccgcg ttcgcaagct tcaaacgatc ggaataaagc ccggacacat cggtggtgaa
      241 aacgtcacag cgcccggact cgtaggcctt gatggcttcg tcgttggtgg cgaatgcgat
      301 cacctcgtac ttcatgttgt tgcccttgaa gtagtcggcg agattctgct cggtggttgt
      361 gccttgctgc acgcagaccg atgcgctgtt caattccagc gcggaattga ccttaaggga
      421 ttttttcacc agaaaacctt gaccgtcata ataggtcacc ccggtaaagt tggcgccgag
      481 cgaggtgtcg cgggaaagcg tccaggttgt attacgcgag agtacgtcga tttcgccgga
      541 ctgcagcgca gtgaagcggt ccttcgctga caacggtacg aatttgactt tggttggatc
      601 gttgaagatt gcggcggcaa ccgcgcggca gacatcgacg tcgagcccgg tccagttacc
      661 cttgtcgtcc ggcgacgaga agcccggcaa gccctggctc acgccgcaag atagcatgcc
      721 ccggtccttg accgtcttga gcgtttgcgc cgttgcggcc tgggtcgaaa aaccgccagc
      781 gagggcaagg gtaacaacca gagatatacg tttcatgcta aagcctttca agggtcgtcc
      841 gggaacgttt gcttatcatc gcgtgcgccg atgggccaat cctgtgatcc actgctgcaa
      901 acgccagcct gacaggcagt tttgacacgc cgtccggtga agcgaaggat cgaagatcgc
      961 ggcaggcccc tcaacgtgcg gcgactgaat agctcccgcg catcacagaa cgactggcga
     1021 gccgggtcaa ggggttgacg gccctcttct gtgtcctgtt actaacaaaa ccagcccgaa
     1081 cccgccgccc ggcgggtttt cgatggggcg ccgcggctgc ggcaaaaggt cttttagggc
     1141 ggcggacaca tgaacgattc aaggggcgat gcaggcacga gcccgcagaa ggcggaaacc
     1201 ttgctggtaa ccgcgggtcg cgacaccaag gcgcagaagg gctttgtcaa tccgccggtg
     1261 gtgcacggtt ccaccgtgct ctacccgacg gcggaagatt tgcacgccca tcgcagcgag
     1321 ttccagtacg gccgcagggg aactccgacc accaaggcac tgcaggaggc gctgatggcg
     1381 ctcgaaggcc cgcaatgcgc cggcgttggc ctggcgccct cgggcctgtc ggcgatcacc
     1441 accaccctgc tcgcggtgct gaaagcgggc gatcatcttc tggtgtgtga caatgcctac
     1501 cggccgtcgc gtaatttctg caat
//
LOCUS       DASWQN010001208         2920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2229960, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001208 DASWQN010000000
VERSION     DASWQN010001208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229960"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..566)
                     /locus_tag="VGC82_20510"
     CDS             complement(117..566)
                     /locus_tag="VGC82_20510"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0425711.1"
                     /translation="MKTRYGLEIRAAISTDAQGLCDLLHGAGQEMSSRALAERLDAIR
                     LDAGAALIAAEWGPPTGLILLHRYRTLAADQPIAQITTLLVAPDERRRGIGRLLVKAA
                     AQAARVAGCGGLELLSAADEEALHDFCRATGFTSVGPRFMRPLRKKG"
     gene            846..2039
                     /locus_tag="VGC82_20515"
     CDS             846..2039
                     /locus_tag="VGC82_20515"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0425712.1"
                     /translation="MAEPAGPLKGFRILDLTTVLFGPFGTQTLGDWGAEVIKIESLSG
                     DTWRTSGQFRNRGMSGQFMAANRNKRSLAVDLKHPDGKGVLRRLIATADALVSNIRPA
                     GLARLGFSYDTCKELNPKIIYAVATGFGQDGPWGARPAFDEIIQAASGFASAMGTDEE
                     PTFVPSLIGDKICGMALTSAVTAALLHRERTGEGQMVEVPMLETLAAFNSIEMFGGYA
                     FVPPIGPSGYKRMKARRPVRTKDGWLTMLPYSGDNWCAFFEAVGHPECIEEYAVRDPV
                     LRARNIDRIYDRMRDIAPTRTTAEWEELLLRIDVPHAAFAKLTEIAEQPHLKAVGMFP
                     ELDHPTEGKIRQARPPTRFSASPAGIHLMPPQLGEHTSEVLREVGFAQEEIDSLIKTK
                     AVRVA"
BASE COUNT          557 a          935 c          882 g          546 t
ORIGIN      
        1 gttactggga gcttgcccgt ctggctgacc gcctggagga attttcgtta tcgcactcat
       61 ctcgcccgtg acgtgtcctc aggcagacgc cccgcgcgac tgcgtcgtga ctccggctac
      121 ccctttttac gaagcggcct cataaagcgt ggtccgaccg aggtgaaccc ggtggctcga
      181 cagaaatcgt gcagcgcctc ttcatccgcc gcggacagta gctccagccc gccgcagccg
      241 gcgacccttg ccgcctgcgc tgctgccttg accagcaatc gaccgatgcc cctccgccgt
      301 tcatccgggg cgaccagcaa ggtggtgatc tgtgcgatcg gttgatcggc tgccaatgtt
      361 cgataccggt gcagcaagat aaggccggtc ggaggtcccc attcggcggc gatcagtgcc
      421 gcgcctgcgt caagacggat ggcgtctagt cgctccgcca aagcgcgtga ggacatctcc
      481 tggccggccc cgtgcagcaa atcgcataag ccttgtgcgt ctgtgctgat ggcggcacga
      541 atttcaaggc cgtaacgcgt tttcaacagc catctccatc gctgtagcca gcaacctgat
      601 ccgactatgg cgcggggata agcacgctac caagtggctt tccacactgg ctggaggcac
      661 gcaacgcacg aggccgcctg ctcgagatgg gtacgtaatc atccggttgc gcctcaacca
      721 aagccgcagc gctatgccag tcggcgctgt gagcgccggt gcatcttatg cccctcacgc
      781 ccggtgtcgc cacacgccat cactttacct atcctgagcc tgacatagat caaggatgaa
      841 ccgcgatggc cgaacccgcc gggccgctga agggcttccg cattctcgac ctgaccaccg
      901 tgttgttcgg cccgttcggc acccagactc tgggcgactg gggtgccgag gtcatcaaga
      961 tagagagcct gagcggcgac acctggcgca cctcaggtca attccgcaac cgcggcatga
     1021 gcggccagtt tatggcggcg aaccgcaaca aacgcagctt ggcagtcgat ctcaaacacc
     1081 ctgatggcaa gggagtgctc cggcggctga tcgccacggc ggacgcgcta gtcagcaaca
     1141 tccgtcccgc cggcctggct cgtctcggtt ttagctacga cacctgcaag gaactcaacc
     1201 caaagattat ctacgcggtg gccaccggct tcggccagga cggcccctgg ggcgctcggc
     1261 cggcctttga tgagatcatt caggccgcct ctgggtttgc ctcggccatg gggactgatg
     1321 aggagcccac gttcgtcccc agcctgatcg gcgacaagat ctgcggcatg gcgctgacct
     1381 ccgctgtcac agcggccctg ctgcaccgcg agcgaactgg cgagggccag atggtcgagg
     1441 tgccgatgct ggaaacgctg gcggccttca acagcataga gatgtttggc gggtatgcct
     1501 tcgttccccc tatagggcca tcgggctaca agcgaatgaa agcgcgtcgg cccgttcgca
     1561 ccaaggacgg ttggctgacc atgttgccct attcgggcga taactggtgt gcgttctttg
     1621 aggcggtcgg gcatccggag tgcattgagg aatatgcggt gcgcgatccg gtgctgcgcg
     1681 cccgcaacat cgacaggatc tacgaccgca tgcgcgacat tgcgccaacc cgaaccacag
     1741 cggaatggga ggaattgctt ctgcggatcg acgtgccgca cgccgccttc gcgaagctga
     1801 ccgagattgc cgagcagccg catttgaagg ccgtaggcat gtttcccgaa cttgatcatc
     1861 ccaccgaagg caagatccgc caggctcgtc ctccgacgcg gttctccgcc agccccgccg
     1921 gcatccacct catgccgcct caactgggcg aacacacaag cgaggtgctg cgcgaggtag
     1981 gctttgctca ggaagagatc gatagcctga ttaaaacgaa ggcagtacgg gtggcgtagg
     2041 accacccacc cttagccgcc cgccggacta cgacggcctg ccgaccaact ttagctttgt
     2101 tctggttgtt atcgggtgct ggcacgccaa cagcgcggtc cacaggctcg ccgcgcgcac
     2161 ccagaggacc cgcatcgcaa tctcgggctg aagatcgtca tcggccatgg gcaaggcgtt
     2221 tccgttcatg ctggaccgca atgcctggcc gaagcgagca cgaacatgta atcccaccga
     2281 acaacgatta cccactggcg ttctttccgg aacaatcgaa ccacaggagg aagcatgcga
     2341 acctccttga tcagtggaat cgccgccctc ggaatgttga ctgcgtgcga cccttacagc
     2401 ccggaccaac gcgccgttgg tggtggggca atcggcgctg gcaccggggc agcagttggc
     2461 ggattggtgg ggggcggacg cggcgctgga accggagcct cgatcggtgg cggggtgggg
     2521 gcggcaggag gggcgctaac gacaccgtcg cgaccctacg aaccttctac cggttatggc
     2581 tacccatcat accgctcctc gcccaatcgg tattaaactg ccgactaagc agtttccact
     2641 cggtggacca acggatgaac tgtacgcttt gccgtcgcgg ggtgctttac ataagttccg
     2701 gcaatacccc ttccgttggt catcccgcgt atgtctgctt agtttgaagc cgatggttct
     2761 gcccgctggc gccaaaggcc aatccccaac cggcgctgtc gccaggagag acagaaccga
     2821 actggacggg gtccaaatta cctccgctct gacaaacatc gtcggccaat ctttggctgt
     2881 ctggtggtcc ccgaatggtg ccttggaccg cgtatgggac
//
LOCUS       DASWQN010001209        10335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1701610, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001209 DASWQN010000000
VERSION     DASWQN010001209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 10335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10335
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701610"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..3264)
                     /locus_tag="VGC82_20520"
     CDS             complement(175..3264)
                     /locus_tag="VGC82_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEY0425713.1"
                     /translation="MNISAPFIARPIATWLLALAVLLSGALGYRALPVSALPEVDFPT
                     IQVVTQLPGASPETTETLITASLERQFGQIPGLLLMTSQSAEGTSQITLQFVLNRSMD
                     SAAQDVQAAINASAGTLPANLPYPPTYSKVNPADAPILTLALTSEGLPIDTISDAADT
                     LLQPKLSEIDGVGRVTVQGNMRPAVRVRIDPARLAGYGLSMEDVRTAVAAANVNGAKG
                     GFDGPRLAFSLGANDQLVDAAAYQNLVIAWRNGAPVRLSAVGSVVSGVENDRVGAIYN
                     GTPAVVLDIQRQPGANIVQTVQAIQQALPKLRNAIPSGIKVSVVTDRTETIRASVRDV
                     QYTLIMSVVLVVLVIFVFLRSARATFIPAVALPLSLIGTFGVMQLLGYGLDNLSLMAL
                     TIATGFVVDDAIVMIENVVRFIERGVPPLEAAFRGAAQIGFTIVSLTVSLVAVFIPLL
                     FMTGVVGRLFKEFSVTLTVAVVVSAVISLTLTPMMCGRLLRPTHNDKPGLVARWSEGA
                     FDRLLAGYRRTLDWSLGHQTLVLIVGVATLGGTLALYAVVPKGFLPRQDTSVLLAVTE
                     AAQSASIPKLVALQTRMAEIIRRDPAVTGVVSFAGAGTINTTPNTGRLTIGLKPVGAR
                     DPMDVVVARMQAAISGIPGITAFFQPVQDIQIGTRVSRTQFQYTLMDTDAAELAAWAP
                     RLRQKLSTLPELLAVASDQQDEGFRTYISVDRDAAMRLGVSMQAIQDTLYDAFGQRQI
                     STIFGQANQYRVVLEADPLWQADPNSLRLLRIAGPNGVQVPLSAIARVERITAPLVIS
                     HQEQFPAVTLSFDLAAGYSLGHAVDAVARAEKDIGMPETITGNYSGDAAEFQSSLSAE
                     PWLILAAIVVIYIVLGVLYESWIHPITILSTLPSAGIGALLALMICGIDLSLVALVGI
                     VLLMGIVKKNAIMMVDFAIEAERTRGLAPLDAMREACLLRFRPIMMTTMAALLGALPL
                     MLESGAGSELRYPLGVTIVGGLLLSQFLTLYTTPVIYLAFERLRLRTSGSIAPAAAE"
     gene            complement(3281..4528)
                     /locus_tag="VGC82_20525"
     CDS             complement(3281..4528)
                     /locus_tag="VGC82_20525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0425714.1"
                     /translation="MDVLTREPVMTRPKSRVRSWSVILLSVLAVAAIGWAIWFFPSAG
                     PSTKGRDREAGQPVPVLTSVAATKNVPIYMDGLGTVQAFYTVTMKAMVDGPLLAVNFK
                     EGQDVHKGDVLAQIDPRTYHAALDNATAKKAQDQAQLANARLDLARQQKLVANNYTSA
                     QQADTAKAQVAQFEALVQQDQAQIDTARTQFGYTTITAPVDGRTGIRLVDAGNIVHAA
                     DATGIVVITQLQPISVLFTLPQQALPAVAKAMAEGSPPVLALAQDATGGAAKLLDTGS
                     LTVLDNQVDATTGTIKLKAVFPNAERRLWPGGFVSVRLRTETAKDAVVVPPSAIQRGP
                     RGSYVFVIDKDATAKRQAVTVGYEDEQGSIIASGLKAGEIVVVDGASRVSDGGKVRMS
                     KPDSAQASGPDRPSAPGTSPQRQ"
     gene            complement(4698..6215)
                     /locus_tag="VGC82_20530"
     CDS             complement(4698..6215)
                     /locus_tag="VGC82_20530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0425715.1"
                     /translation="MRPSFRIWLVAAAVLLAGCDIGRDYQRPGLDLPEAYRATAESAA
                     ASWPSADWWQGFRSPELNGLIEQARINNFDIAAAIARVRQADAQVRIAGAPLLPDISG
                     NASGNWQHQGLGTGSSSRIGSTTRSGGSNASLDFHSYSVGLSAAYDLDFWGRNRAARQ
                     SAVASAMFSRFDQQTVALSVVTNVANTWFTALSLADRLAVARRSLADAEQTLSVIRGR
                     FDAGTANQLDIAQQESLVAAERALIPGLISQMEQELIALGILIGQPPERVSARPGTLT
                     ALALPLVAPGLPSELLTRRPDIASAEAQLISQNFSITVARAAFFPSIQLTASAGYQAP
                     ALNRLITPGGALASLAAGLTLPIFDGGTLRGQLDLAKGRYDELLADYRKTVVQAFTDV
                     DTALTAWRFASEQENLQRIAVDAARRAATIARAQMAAGTVDVLTVLTTETTLFNDEDT
                     LVQVRLARTQALLNLYKALGGGWTASSQPLGQQFPGLTPGMVPGGVALPLGGNLR"
     gene            6509..7261
                     /locus_tag="VGC82_20535"
     CDS             6509..7261
                     /locus_tag="VGC82_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425716.1"
                     /translation="MTDIILETEGLTRDFGGFLAVDGVNLRVETGTIHALIGPNGAGK
                     TTCFNLLTSFLPPSRGTIKFKNRDITGMKPAMVARLGLIRSFQISAVFPHLTALENVR
                     LGLQRARGASFDFWRSDTALKEYDDRARELLFDVGLSGFAETVVADLSYGLKRALEIA
                     TTLALEPEMMLLDEPTAGMAHGDVDRIVDLIKRIRKGRTILMVEHNLSVVEGLCDKIT
                     VLTRGKVLAEGDYDTVSKNPEVIAAYLGTVDA"
     gene            7254..7949
                     /locus_tag="VGC82_20540"
     CDS             7254..7949
                     /locus_tag="VGC82_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425717.1"
                     /translation="MLELKGLNSWYGESHILHGVDFEVREGEVVTLLGRNGAGKTTTM
                     KSIMGLVPRREGSVKFRGAETIGLMPNQIARTGIAICPEERGIFGSLSVTENLMLPPI
                     VAPGGLSVDEIYTLFPNLRERARTAHGTKLSGGEQQMLAIARILRTGAKMLLLDEPTE
                     GLAPVIVQQIGRTIREIKQRGFTVLLVEQNFRFAATVADRHYVMEHGKIVDMIPNHLL
                     DSSMDKLHEYLGV"
     gene            8119..9315
                     /locus_tag="VGC82_20545"
     CDS             8119..9315
                     /locus_tag="VGC82_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0425718.1"
                     /translation="MLPVVRPARAQTSATTAPTLRIGVLNDQSGPYTNTGGITSVICA
                     KQALEDFGVSGKGMHVEVISADHQNKPDLAVSIARQWFDRDGVDVLLDVPTSSVALAL
                     QSVVREKNKVYLNVGAASSALTGSQCSPNFIHWVYDTYMLSKSTGGAMVKAGGDSWYF
                     LTADYAFGKQLQDDTSTLVKAAGGKVLGSAPYPFPGTTDFSSFLVQAQSSGAKVLGLA
                     NAGGDTVNSIKQAHEFGVNNSMKLAALLMFITDVHALGLDVAGGLNLTESFYWDLNEG
                     TRAFTKRVLPKTPNNYPNMIHAGCYSATLHYLKTVADMGAAEARKDGVATVNRMKAMP
                     VEDDCFGRSKIREDGRNMVPAYLFEVKAPGESKGPWDYYKLKVTTPGDEAYRPLAEGH
                     CSIVKA"
     gene            9386..10273
                     /locus_tag="VGC82_20550"
     CDS             9386..10273
                     /locus_tag="VGC82_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0425719.1"
                     /translation="MFMIFGIPGPLLFGQLLLGLINGAFYAMLSLGLAVIFGMLNIIN
                     FAHGALFMMGAFASWMLLNYVGIGYFPALILAPLLVGAFGILLEKTIISRLYKLDHLY
                     GLLLTFGLSLVIEGLFRNAYGSSGMPYRIPAVLQGATNLGFMYMPNYRGWVVLAALVV
                     CISTWAIIEKTSLGATLRAATENAPMVQAFGVNVPRLITLTYGGGVALAAFAGVLAAP
                     IYSVNPNMGTNFINTVFAVVVIGGMGSIMGSILTGFGLGVVEGLTKVFYPQASATVVF
                     VVMVIVLLVRPRGLFGKAA"
BASE COUNT         1850 a         3359 c         3293 g         1833 t
ORIGIN      
        1 gcgagagggc aggcagaagc ctggacaacg ctggacaaag cgtgcgcgat acgctggatc
       61 cgccgcgcgg tcccatcgaa tccgcgggac gcaaggtgga tcgcacgctg gggaattgac
      121 ttccggttta gaaggcttcg aactcggtca tcaccggtgg atgcggacat tgcgctactc
      181 cgccgccgcc ggcgcgatcg atcctgacgt tcgcagccgc aaccgctcga atgccaggta
      241 gatcaccggc gtcgtgtaga gcgtcaggaa ctgcgacagc agcaagccgc cgacgatcgt
      301 cacgcccagc gggtagcgca gttccgaccc ggcaccgctc tccagcatca gtggcagcgc
      361 tccgagcaac gcggccatcg tcgtcatcat gatcggacgg aaccgcagca ggcacgcctc
      421 gcgcattgca tccagcggcg cgaggccgcg cgtgcgttcg gcctcgatgg cgaagtccac
      481 catcatgatc gcgttcttct tcacgatacc catcagcagc acgatcccga ccaaagccac
      541 cagcgacagg tcgatcccac agatcatcag cgccagcagc gcaccaatcc cggcggacgg
      601 cagcgtggac aggatggtga tcggatggat ccagctttcg tacagcacac ccagcacgat
      661 gtagatcacc acgatcgcgg ccaggatcag ccacggttcg gctgacagcg acgactggaa
      721 ttccgcggca tcgccggagt agttgccggt gatggtttcc ggcatcccga tgtccttctc
      781 cgcccgcgct accgcatcca cggcgtggcc caacgaatat ccggccgcga ggtcgaagct
      841 caacgtgacc gccgggaact gctcctgatg cgatatcacc agcggcgcgg tgatccgctc
      901 gaccctggcg atagcggaca acggaacctg tacgccattc ggcccggcga tgcgcagcaa
      961 tcgcaggctg ttggggtcag cctgccacag cgggtccgcc tccagcacca cgcggtactg
     1021 gttggcctgg ccgaagatgg tggagatttg ccgctgtccg aacgcgtcat acagcgtatc
     1081 ctggatcgcc tgcatcgaca cgcccagccg catcgccgcg tcgcggtcca cacttatata
     1141 ggtgcggaaa ccctcgtcct gctggtcgct cgcgaccgca agcagttccg gcagggtgct
     1201 tagtttctgc cgcagccgcg gcgcccatgc tgcaagctcc gctgcatcgg tgtccatcag
     1261 cgtgtactgg aactgcgtcc ggctaacgcg cgtgccgatc tggatgtcct gcaccggctg
     1321 gaagaacgcg gtaatgccgg gaatgccgga aatggcggcc tgcatccgcg cgaccacgac
     1381 atccatggga tccctggcac ccacgggctt caggccgatc gtcaggcggc cggtgttggg
     1441 agtggtgttt atggtgccgg cgccggcgaa cgacaccacg ccggtcaccg ccggatcgcg
     1501 gcggatgatc tccgccatcc gcgtctgcaa cgcgaccagc ttggggatcg aagcgctttg
     1561 tgccgcctcg gtcactgcga gcaaaacgct ggtgtcctgg cgcggcagaa agcccttggg
     1621 caccaccgca taaagcgcca gcgttccgcc cagggtcgcg acgcccacga tcaaaaccaa
     1681 tgtctggtgt ccgaggctcc aatccagggt acgccggtag cctgccagca gccgatcgaa
     1741 agccccctcg ctccagcgcg ccacgaggcc gggcttgtca ttgtgggtcg gccgcagcag
     1801 ccgcccgcac atcatcggcg tcagcgtcag cgaaatgact gccgacacca cgacggccac
     1861 ggtcagcgta accgagaact ccttgaacag ccgccccacc acgccggtca tgaacagcaa
     1921 gggaatgaac accgcgacca gcgacacggt caaagacacg atggtgaagc cgatctgcgc
     1981 ggcgccgcgg aatgctgcct ccagcggagg cacgccgcgt tcgatgaagc ggacgacgtt
     2041 ctcgatcatg acgatggcgt cgtccacgac gaagccggtg gcgatggtca gagccatgag
     2101 tgacaggttg tccagcccgt agcccagcaa ctgcatgacg ccgaaggtgc cgatcagcga
     2161 cagcggcagg gcgacggcgg ggatgaacgt cgcgcgggca ctgcgcagga acacgaagat
     2221 gaccagaacg accagcacca cggacatgat cagcgtgtac tgcacgtcgc gaaccgatgc
     2281 ccggatcgtc tccgtgcggt ccgtcacgac ggaaaccttg atccccgatg ggatcgcgtt
     2341 gcgcaacttc ggcaacgcct gctggatcgc ctgcaccgtc tggacaatgt tcgcgcccgg
     2401 ctgacgctgg atatccagca ccaccgccgg tgtgccattg tagatcgcgc cgacgcggtc
     2461 gttctccacc ccgctgacca cgctgcccac cgcggacagc cggaccggcg cgccgttccg
     2521 ccaggcgatc accaggttct ggtacgccgc cgcatccacg agctgatcgt tggcgccgag
     2581 cgaaaatgcc agcctggggc cgtcgaagcc tcccttggca ccgtttacgt tggccgccgc
     2641 aacggcggtg cgcacgtcct ccatcgacag gccgtagccg gccagccttg ccgggtcgat
     2701 ccgcacgcga accgccggcc gcatgttgcc ctggacggtc acgcgcccca caccgtcgat
     2761 ttccgagagc ttcggttgca gcagggtgtc ggcggcatcg ctgatggtgt cgatcggcaa
     2821 gccttccgac gtcagcgcca gggtcaggat tggcgcatcg gccgggttga ccttcgagta
     2881 ggtcggcggg tagggcaggt tcgccggcaa cgtgccggcc gaggcgttga tcgccgcctg
     2941 cacatcctgc gccgccgaat ccatcgaccg gttcagcacg aactgcagcg tgatctgact
     3001 ggtcccttcg gcactctgcg aggtcatcaa cagcagcccc ggaatctggc cgaactggcg
     3061 ttccagcgac gcggtgatca gcgtctcggt ggtttcgggg ctggcgccgg gaagctgtgt
     3121 gacgacctgg atggtcggga aatcgacctc cggcagagcc gagaccggca gcgcccgata
     3181 acccaacgct cctgacaaca ggaccgccaa cgccagcagc caggtggcga tgggacgggc
     3241 gatgaagggt gccgagatat tcattttctc gctgccgggc ctactgccgc tgcggactgg
     3301 tgccgggagc gcttggccga tccggcccgg atgcctgggc ggagtccggc ttgctcatcc
     3361 gcaccttgcc gccatccgat acgcgtgagg ctccgtcaac gacgacaatt tccccagctt
     3421 tcagaccgct ggcgatgatc gagccctgtt cgtcttcata gccgacggtt accgcctggc
     3481 gtttcgccgt cgcatcctta tcgatcacga aaacatacga accgcgaggg ccgcgctgga
     3541 tcgccgaagg cggcacgacg accgcgtcct ttgccgtctc ggtgcgcagg cggacactga
     3601 caaacccgcc cggccacaac cggcgttcgg cgttgggaaa aaccgccttc aacttgatcg
     3661 tcccggtcgt ggcatcgacc tggttgtcca gcacggtcag cgagccggta tccagcagct
     3721 tcgccgcgcc gccggtcgca tcctgtgcca gcgccagtac cggcgggctg ccttccgcca
     3781 tcgccttcgc cactgccggg agcgcctgct gcggcagcgt gaacagcacc gaaatcggct
     3841 gtagctgcgt aatcacgacg atgccggtcg catcggcggc atgaacgata ttcccggcat
     3901 cgaccaaccg gatccccgtc ctgccatcga ccggagcggt tatcgtggtg tagccgaact
     3961 gggtgcgcgc ggtatcgatc tgcgcctggt cctgctgcac cagggcctcg aactgcgcga
     4021 cctgagcttt cgcggtatcg gcctgctgtg ccgaggtata attattcgca accagcttct
     4081 gctgccgcgc cagatccagc cgggcatttg ccagttgcgc ctgatcctgc gctttcttgg
     4141 cggtcgcgtt gtccaaggcg gcgtgataag tgcgcggatc gatctgcgcc agcacatcgc
     4201 ctttgtgcac gtcctggccc tctttgaagt tcaccgcgag caacgggcca tcgaccatgg
     4261 ctttcatcgt cacggtgtag aacgcctgaa ccgtgcccaa tccgtccatg tagatcggca
     4321 cgttcttggt tgccgcaacg ctggtcagca cgggcacagg ttggccggcc tcgcgatcac
     4381 ggcccttggt tgaaggaccg gctgacggaa agaaccagat cgcccagccg atggccgcga
     4441 ccgccagcac gctcagcagg atcacgctcc agctgcgcac gcggcttttg gggcgggtca
     4501 tcactggttc acgggtcaga acgtccatgg aatgcagcct tgttgtaggg tagcagccga
     4561 cgcccgcgcc ggctgggtgg gagtcttgct ttcgcccgcc gccgccccga cggcgtatct
     4621 gccagcgcca gtgccactgc cagtgccagc ggggatcgcc ccacgatatc ggtatggttc
     4681 gcggtttcgc cacgcgttca tcggagattt cctccaagcg gcagcgccac gccgccagga
     4741 accatgccgg gcgtcagccc gggaaattgc tgaccgagcg gctggctgga agccgtccag
     4801 cccccgccaa gcgccttata aaggttgagc aacgcctggg tgcgggcgag gcgaacctgc
     4861 accagcgtat cctcgtcgtt aaacagtgtc gtctccgttg tcagcacggt taagacatca
     4921 acggtcccgg cggccatctg cgcccgcgcg atcgttgctg cccggcgggc ggcatccacg
     4981 gcaatgcgct gcagattttc ctgctcgctg gcgaagcgcc aggcagtaag cgcggtatcg
     5041 acatcggtga acgcctgcac caccgtcttg cggtagtccg ccagcaactc gtcatagcgg
     5101 cccttcgcca gatcgagctg gcctcgcagg gtcccgccgt cgaagatcgg cagcgtcagc
     5161 cccgcggcca gcgaggccaa cgcaccgccc ggcgtgatca gccgattcag tgccggcgcc
     5221 tggtacccgg cgctcgcggt caactggatg ctggggaaaa acgccgcgcg agcgaccgtg
     5281 atactgaaat tctgtgagat aagttgcgct tcggccgatg cgatatcagg ccggcgcgtc
     5341 aataattcgg atggcagacc cggcgccacc agtggcaagg ccaaggccgt cagcgtgccg
     5401 ggccgggcgg agacccgctc cggtggctgt ccgatcagga tgccaagagc gatcagttcc
     5461 tgctccatct ggctgatgag ccccgggata agggcacgtt ccgccgccac cagcgactcc
     5521 tgctgggcga tatccaactg gttggcggta ccggcgtcga agcggccgcg gatcacgctc
     5581 agggtctgtt ccgcatccgc cagactgcgg cgcgccaccg ccagccggtc ggccagggat
     5641 aaagcggtga accaggtgtt ggcgacgttg gtgacgacgc tcagcgcaac ggtctgctgg
     5701 tcgaaccggc tgaacatggc ggaggccacg gctgactgcc tggcggcacg gttgcgtccc
     5761 cagaaatcca gatcgtaggc cgcgctgagg ccgacgctgt agctgtgaaa gtccaggctg
     5821 gcgtttgatc cgccactacg ggtcgtgctg ccgatgcggc tgctgctgcc ggtgccgagg
     5881 ccttgatgct gccagttgcc gctggcgttg ccgctgatgt cgggcagcag cggggcgcca
     5941 gctatgcgca cctgggcatc cgcctgccga acccgggcga ttgccgccgc gatgtcgaaa
     6001 ttgttaatac gtgcttgttc gatcagccca ttcagctccg gggagcgaaa accttgccac
     6061 cagtccgccg acggccagga ggccgccgcg gattccgcgg tcgcgcgata agcctccggc
     6121 aggtcgaggc cgggccgctg gtaatcccgg ccgatgtcgc agcccgccaa cagcacggcg
     6181 gctgcgacca gccaaatccg aaaactcggt ctcacgcaag gacatcctga tgacgcgcga
     6241 caagaccgcc ggtggcgcgg tccgtatttt tgcgcggtgt tgcggcatag atctggtggc
     6301 cttatgtaac ggattcatgt cccgccagtc cagaagtaga tgaaaacttg gccatgccaa
     6361 gtgggtccca atgatcccaa cggtgtcccg ggaacctatg ggccgcgacg ttacggcttt
     6421 cacacccgga ggcggccttg cacgctggcc cgctatatgc aaaacgcccc cgcacaacgt
     6481 aaccaattcc accgaggaga cgtccgggtt gacagatatc atcctggaga cagaaggcct
     6541 gacgcgcgac ttcggcggct tcctggccgt ggacggggtg aatttgcgcg tcgagaccgg
     6601 cacgatccat gcactgatcg gccccaacgg ggcgggcaag acgacgtgct tcaacctgtt
     6661 gacgtccttc ctgccgccgt cgcgcggcac catcaagttc aagaaccgtg acattaccgg
     6721 catgaagccc gccatggtgg cgcgcctggg gctgatccgc agcttccaga tctcggcggt
     6781 gttcccccat ttgaccgcgc tggagaacgt gcggctgggg ctgcaacgcg cccgcggcgc
     6841 cagcttcgac ttctggcgtt cggacaccgc cctgaaggag tatgacgacc gcgcccgcga
     6901 attgttgttc gacgtcggcc tgtcggggtt cgccgagacg gtggtcgccg acctgtcgta
     6961 cggcctcaag cgcgccctgg agatcgccac caccctggct ctcgaacccg agatgatgtt
     7021 gctggacgaa cccaccgccg gcatggcgca tggcgacgtg gatcgcatcg tcgatctgat
     7081 caagcgcatc cgcaaaggcc gcaccatcct gatggtcgag cacaacctgt cggtggtcga
     7141 aggcctttgc gacaagatca ccgtgctgac ccgcggcaag gtgctggccg agggcgacta
     7201 cgacacggtg tcgaagaacc ccgaggtgat cgccgcttat ctgggaaccg tcgatgcttg
     7261 agctgaaggg tctgaatagc tggtatggcg agagccacat cctgcacggc gtggacttcg
     7321 aggtgcgcga gggcgaagtc gtcacgctgc tgggccgcaa cggcgccggc aagaccacga
     7381 cgatgaagtc gatcatggga ctggtgccgc gccgcgaagg cagcgtgaag ttccgcggcg
     7441 ccgagacgat cgggctgatg ccgaaccaga tcgccaggac cggcatcgcc atctgtccgg
     7501 aggaacgtgg gattttcggc tccctgtcgg tcaccgagaa cctgatgctg ccgccgatcg
     7561 tcgcccccgg cggcttgtcg gtcgacgaga tctacacgct gtttcccaac ctgcgggaac
     7621 gcgccaggac cgcccacggc accaagctat cgggcggcga gcagcagatg ctggcgatcg
     7681 cccgcatcct gcgcaccggt gcgaagatgc ttctgctgga cgagccgacc gagggcctcg
     7741 cgcccgtcat cgtgcagcag atcggccgca ccatacggga gatcaagcaa cgcggcttca
     7801 cggtgctgct ggtggagcag aacttccgct tcgctgcgac ggtcgccgac cggcattacg
     7861 tcatggagca cggcaagatc gtggacatga tcccgaacca cctgctcgac tccagcatgg
     7921 acaagctgca cgagtatctc ggtgtttgac agcggccgag gacaaagggc ggcgtgacta
     7981 ttatcggcgg attgcatcca tactggcccg caaggcgttc cggccactgg ataggcggcc
     8041 ctttcgtgat caaagcatag aggtttcgtg atggcactca ctcgtcgttc cgctcttcgt
     8101 acagtggccg cggctactgt gctgcctgtc gttcgtcccg cccgagcgca aacatcggca
     8161 accacggcgc cgacgctcag gattggcgtt cttaacgatc agtcgggccc atacaccaat
     8221 accggcggta tcacctcggt gatctgcgcc aaacaggcgc tggaggattt cggcgtctcc
     8281 ggcaagggga tgcatgtcga agtgatctcc gcagatcacc agaacaagcc ggaccttgcg
     8341 gtctcgatcg ctcgccaatg gttcgaccgc gacggcgtcg atgtcctgct ggacgtgcca
     8401 acgtcatcgg tggcgctggc actgcagtcc gtggtccgcg agaagaacaa ggtctatctc
     8461 aatgtcggtg cggcttcctc ggcgctgacc ggctcgcagt gctcgcccaa ctttatccat
     8521 tgggtgtacg acacctatat gctgtcgaag tccaccggcg gcgcgatggt gaaggcgggc
     8581 ggcgattcct ggtatttcct gaccgccgac tatgcgttcg gcaagcaact gcaggacgac
     8641 acctcgacct tggtgaaagc ggcgggcggc aaggttcttg gttccgcgcc ctatccgttc
     8701 cccggcacga ccgatttttc ctcgttcctg gtgcaggcgc agtccagtgg cgccaaggtg
     8761 ctggggctgg ccaatgctgg cggcgatacc gtgaactcca tcaagcaggc gcatgagttt
     8821 ggcgtgaaca actcgatgaa gctcgcggcg ctgctgatgt tcatcaccga cgtgcatgcg
     8881 cttggcctgg acgtggcggg cgggctgaac ctgacggaaa gcttctactg ggacctgaac
     8941 gagggcaccc gggcgttcac caagcgggta ctgccgaaga ccccgaacaa ctatcccaac
     9001 atgatccacg ccggctgcta ttccgccacg ctgcattacc tgaagacggt tgccgacatg
     9061 ggcgctgccg aggccagaaa ggacggcgtc gcgactgtca accgcatgaa ggcgatgccg
     9121 gtggaggacg attgcttcgg ccggagcaag atccgcgaag acggccgcaa catggtcccg
     9181 gcctacctgt tcgaggtcaa ggcgccaggc gaaagcaaag gcccctggga ctactataag
     9241 ctgaaggtga cgacgccggg cgacgaggcc tatcggccgc tggcagaggg ccattgctcg
     9301 atcgtcaagg cataaaaagg aatcaaccgg ggacgggcgc gtccccgggg aagcagcgat
     9361 tgggggaaac cgtggtgaaa caaccatgtt catgatcttt ggcattcccg gtccgctgtt
     9421 gttcgggcag cttctgctcg ggctgatcaa cggtgcgttc tacgccatgc tgtcgctggg
     9481 gctggcggtc atcttcggca tgctcaacat catcaacttc gcccatggtg cgctgttcat
     9541 gatgggcgct ttcgcctcct ggatgctgct gaactatgtc ggcatcggct acttcccggc
     9601 gctgatcctg gccccgctgc tggtcggggc gttcggcatc ctgctggaga agaccatcat
     9661 cagcaggttg tacaagctcg accatcttta tggcctgctg ctgacgttcg gcctttcgct
     9721 ggtgatcgag ggcctgttcc gcaatgccta cggcagctcc ggcatgccct atcgcattcc
     9781 cgcggtgctt cagggagcaa ccaacctcgg cttcatgtac atgccgaact accgcgggtg
     9841 ggtggtgctg gcggcactgg tcgtttgcat ctcgacctgg gcgatcatcg agaaaacgtc
     9901 gctcggcgcc accctgcgcg cggcgaccga gaacgcgccg atggtgcagg cgttcggcgt
     9961 caatgttccg cgcctgatca cgctgaccta cggcggcggc gtcgcgctgg ccgcgtttgc
    10021 cggcgtgctg gcggcgccga tctactcggt gaaccccaac atggggacca acttcatcaa
    10081 cacggtgttc gccgtcgtgg tgatcggcgg catgggctcg atcatggggt cgatcctgac
    10141 gggcttcggc ctaggcgtgg tcgagggcct gaccaaggtg ttctatccac aggcctcggc
    10201 caccgtcgtt tttgtcgtga tggtgatcgt tctgctcgtc aggccgcgcg gcctctttgg
    10261 aaaggccgcc tgatgttcgg attttcgcgg tcgcagtcca tcgccttcgt ggtgatgctg
    10321 gtgttgatcg tcgtc
//
LOCUS       DASWQN010001210         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_776560, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001210 DASWQN010000000
VERSION     DASWQN010001210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776560"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..787
                     /locus_tag="VGC82_20555"
     CDS             98..787
                     /locus_tag="VGC82_20555"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEY0425720.1"
                     /translation="MNISIGSLLEGATQASGAVAIIDVFRAFTTAAVVLANGASRIIM
                     VSTVKEALALRRRGVGQVCIGEVRGRAPDGFDFDNSPFEVSKLDFASKTIIQRTSAGT
                     QGIVAASRAKSLYAASLVTAKATARVLAAAGERIVLVALGKNGTERTDEDELCALHLR
                     NLLQGRSGDPEAVRRLILAGGEIVRFNDPARPHLHPEDVDIALDVDRHDFAVRVTTED
                     GLAVACVERTG"
     gene            905..2062
                     /locus_tag="VGC82_20560"
     CDS             905..2062
                     /locus_tag="VGC82_20560"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0425721.1"
                     /translation="MPFPPETDVLIVGAGPTGLALATCLASHRVSFVLVDRLEMPQAT
                     SRAAAVHARTLEVLEPFGVTEALVNAGRPMAAAVIRDRDKTLLRIDFGQLRSRYRFIL
                     TLPQNETEAILTNRLALLGGAIERGQEAVALVQDDGHATVTLRDASGSSTQVRARYVV
                     GADGYRSLVRQAAGIGFTAGTYDETFILADVHMDWPWPDELGLFLATDGMALVAPFSG
                     DRFRVIATVDQAPAQPGMADVQAVLDRRGPRSSPIRVRDMIWSSRFHIHHGVAAHYRA
                     NRTFVAGDAAHVHSPAGGQGMNIGIQDAVALGDRLAAVIGGRQPDAYLDGYENERRPV
                     ARRVVAMTDQMTRMGTLKGPIRQGLRNIGLRAAGHLPMLLRMIANRMAELS"
     gene            2163..3257
                     /locus_tag="VGC82_20565"
     CDS             2163..3257
                     /locus_tag="VGC82_20565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEY0425722.1"
                     /translation="MIPAYVGSEIYRHSSYGPKHPLAVPRVSVCTDLCRALGWLPDSV
                     YHEAPMATEAELSRFHDPAYLAALRQAEADQAVSDDVRARFRIGAEGNPVYREVYRRP
                     AISAGGVMLAARLAASGGIVHCPGGGTHHGRSDRASGFCFLNDPVLGLLTWLDHGLDN
                     IVYLDIDAHHGDGVQDAFHDDPRVFTISLHEAKRWPFTGAADDRAGGAARNFPLPAGC
                     NDCEFAHVIENAVLPLIEARRPQAIMLQAGADGLEEDPLAKLSLSNNSHRTVVRAMME
                     RSSRLVVLGGGGYNPWTVGRCWALIWGTLNNFPIPERLNPEAETSLRALRFDRAAGRN
                     PPEHWFSTLLDQPRDGPVRDAMRQLTQAGN"
BASE COUNT          583 a          981 c         1138 g          612 t
ORIGIN      
        1 ttcgaagcgt gtgaagcatg cgatgagaca aagggttgga gcatttgcag cctgtactgt
       61 cgggcggaaa tgctctggct gtgatacagg gacgtccatg aacatatcga tcggcagcct
      121 gctggaaggg gccacgcaag catccggtgc cgtcgccatc atcgacgtct tccgggcgtt
      181 caccacagcc gccgtagttc tggccaatgg cgcgtcgaga atcatcatgg tcagcacggt
      241 gaaggaagcg ctggcgctgc gccgccgcgg cgtcgggcaa gtttgcatag gtgaggttcg
      301 cggtcgcgca ccggacggct tcgacttcga caactcgccg ttcgaggtgt cgaagcttga
      361 cttcgccagc aaaacaatca tccagcggac cagtgccggt acccaaggta tcgtggcggc
      421 ttccagggct aaaagcctct acgccgcgtc cctggtgaca gccaaggcga ctgctcgggt
      481 gcttgccgcg gcaggggagc ggatcgttct cgtcgcgttg gggaagaatg ggacggagcg
      541 gaccgatgag gacgaactat gtgccctgca tttgcgtaac ctgctgcaag gcaggtccgg
      601 tgatcccgaa gcggtacgtc gattgattct ggccggcggt gaaatcgtac gcttcaacga
      661 tccggcgcga ccgcacttgc atcccgagga cgtggacatc gctcttgacg tagaccgcca
      721 cgattttgcg gttcgggtga caaccgagga cggccttgcc gttgcctgtg tcgagcgaac
      781 cggctgaacc gcgaagcgcg aagcggcacc cgcctgccgg ttgcggatca agggattccg
      841 agggcgaatg acctagttgg cggtgaatgt gttacctgat acgtcttctt gagggatata
      901 ggccttgcca tttccacccg aaaccgacgt tctgatcgtc ggggcggggc ccacgggcct
      961 tgccctcgcc acatgcctag cgagccatcg cgtatccttc gtactggtcg atagactgga
     1021 gatgccgcag gcaacctccc gcgccgccgc ggttcatgcc cggacgctgg aggttctcga
     1081 gccgttcggt gtaaccgagg ctctggtcaa tgcggggcgg ccgatggcgg cagccgttat
     1141 ccgggatcgc gacaaaacct tgctgcggat cgacttcggc cagctccgtt cccgctaccg
     1201 gttcatcctg acgctgccgc agaacgagac cgaggcgatc ttgacgaatc ggctggcttt
     1261 gttgggcggc gcgatagaac gggggcagga ggccgtggca ctggtccagg atgacggtca
     1321 cgccaccgtc acgctgcgcg acgcgtccgg ttcgagcacg caggttcggg ctcgttatgt
     1381 cgtaggggca gatggctatc gcagcctggt tcgacaggcg gccggaatcg gattcacggc
     1441 gggaacctat gatgagacgt tcattcttgc ggacgtgcac atggactggc cttggccgga
     1501 tgaattgggg ctgtttctgg caacggacgg catggccctg gtggcgccgt tcagcggcga
     1561 ccggtttcgc gtcattgcca cggtggatca ggcgccggca cagcccggta tggccgatgt
     1621 gcaggcagtc ctcgaccggc gcggcccgcg gtcgtcgcca atccgggtgc gggacatgat
     1681 atggtcgtcg cggttccaca tccatcatgg cgtggcggca cattatcggg ccaatcgaac
     1741 gttcgtggcg ggagatgcgg cacatgtgca tagcccggcg ggcgggcagg ggatgaatat
     1801 cggcatccag gacgcggtcg ccctgggcga tcggctggct gcggtcatcg gcgggcggca
     1861 gccggacgcc tatctggatg gctatgaaaa cgaacgccgg ccggtggcgc gccgggtggt
     1921 cgcgatgacg gaccagatga cccggatggg gacactgaaa ggtcccattc gacaaggtct
     1981 ccgcaacatc ggccttcgcg ccgcaggtca tctgccgatg ctgctgcgca tgattgcgaa
     2041 ccgcatggcg gagctgagtt aggtccgcag atgctgggtc agcgacggac gaggatgcaa
     2101 tcaggccggc gtggggacga accgatgcct gtgccctctc accctcaccc gatggcgcgc
     2161 ccatgatccc ggcctacgtc ggcagtgaaa tttaccgcca ctcgagctat ggcccgaagc
     2221 atcccctcgc tgtcccgcga gtttccgtct gtaccgattt atgccgagcg ctgggttggt
     2281 tgccggacag cgtgtatcac gaggcgccga tggcgacaga ggcggaactg agccgtttcc
     2341 acgatccggc gtatctcgcc gcgctgcgcc aggcagaggc ggaccaagca gtcagtgacg
     2401 atgtccgtgc tcggtttcgc atcggcgccg agggcaatcc ggtgtatcgg gaggtatatc
     2461 gccgcccggc gatctccgcc ggcggggtca tgctggcagc caggttggcg gcgtctggcg
     2521 ggatcgtcca ttgcccgggc gggggcaccc accacggccg gtccgatcgg gcgtcggggt
     2581 tctgcttcct gaacgatccg gtgctggggt tgctgacatg gctggaccat gggctggaca
     2641 acatcgtgta tctcgacatc gacgcgcatc atggcgacgg cgtgcaggac gcgttccatg
     2701 acgatccgcg ggtgttcacg atcagcctcc atgaagcgaa gcgctggccg tttaccgggg
     2761 cggcggatga ccgggccggc ggggcggcgc ggaattttcc gctgccggcg gggtgcaacg
     2821 actgcgaatt cgctcatgtg atcgaaaatg cggtgctgcc gctgatcgag gcgcgccggc
     2881 cgcaggcgat catgctgcaa gctggggcgg atgggctgga ggaagacccg ctggcgaagc
     2941 tctcgttgtc caacaacagc caccgcaccg ttgtgcgcgc gatgatggag cggtcgtccc
     3001 gtctggtcgt cttgggcggc ggcggctaca atccctggac cgtgggccgc tgttgggcac
     3061 tgatctgggg tactttgaat aactttccca taccagagcg tttgaacccc gaagcggaaa
     3121 ccagcctgcg agcactgcgc ttcgatcggg cggccggacg caatccgccg gaacactggt
     3181 tctcgacgct actcgatcaa ccgagggacg gaccggtgcg cgatgctatg cggcaactga
     3241 cccaagccgg caactgaccc aagccggcaa ctgacgcaag agagtatgac tgcatgaaat
     3301 ggcgctatct gctg
//
LOCUS       DASWQN010001211         4875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1438650, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001211 DASWQN010000000
VERSION     DASWQN010001211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438650"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VGC82_20570"
     CDS             <1..1030
                     /locus_tag="VGC82_20570"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY0425723.1"
                     /translation="TVGYALPEDMERMFMAVRNRVPGIEKVVLSTHNHNDLGLAVANT
                     LAAIRAGVRQVESTINGIGERAGNASLEEVVMAIRTRQDAIPRVNRINTQSLLRVSKL
                     LATITGFDVQPNKAIVGRNAFAHEAGIHQDGVLKHAGTYEIMTPESVGWAKNSLVLGK
                     HSGRAAFRDKLKTLGYTIGDNKLNDAFRRFKDLADRKKVVFDEDIVALVDDEVMRDHE
                     RIRFVSLDLHAGSKGPARAELELEIDGAMKIAAASGNGPVDATFNAIQAIFPHTANLV
                     LFSVGAVTEGTDAQAKTTVRLEENGKMVDGQGADSDTIVASARAYIHALNKLMVKRLR
                     TEPAALSA"
     gene            complement(1111..1335)
                     /locus_tag="VGC82_20575"
     CDS             complement(1111..1335)
                     /locus_tag="VGC82_20575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425724.1"
                     /translation="MRAMIVLGLLVLLTACSGDPASYGITGPGIQKLPVGPAADTPDS
                     SPTPGISTTGSSYGPSNRPTTGGSGFWGYN"
     gene            1506..2048
                     /locus_tag="VGC82_20580"
     CDS             1506..2048
                     /locus_tag="VGC82_20580"
                     /inference="COORDINATES: protein motif:HMM:NF024317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 family protein"
                     /protein_id="HEY0425725.1"
                     /translation="MRFCLLAVWLAAGLIGGCARKPLDTVLGGSGPTLDPVAFFSGHT
                     HSGGVIESRSGAPTEWIVTDSLGAQDATGRLRMVQHLAFQDGTTQERTWTMWRSGPHL
                     FMATANDMVGSATGQSNGRIFHWQWVLARAPGNALSNVTMEQWMYQMDDDSLLIRTSV
                     SKFGFILAEVTEQFKRTRKD"
     gene            complement(2204..3994)
                     /locus_tag="VGC82_20585"
     CDS             complement(2204..3994)
                     /locus_tag="VGC82_20585"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY0425726.1"
                     /translation="MESATVDFSLYKEALVVLGAAGIVIPLFHRLRVSAVLGFMLIGL
                     VVGPFGLGSLAGYFPWLGVITIHDNASIEPIAQLGVVLLLFMIGLELSFERLWVMRRL
                     VFGLGSLQVAACALVVGGAALLSGYDRTAALVLGLTLAMSSTAVVIQVLSDEHRLSTP
                     TGRASFAVLLFQDLAVVPALFALGALDPARHDSGGAGFGLAIGRAVAAVVVIVVLGRV
                     VLRPLFRSVARTRSEELFVAACLLVVIASGLATAAAGLSMPLGALIGGLLLAGTEYRR
                     QVEVTIAPFKGLFLGVFLLSIGMSLDIRTIGAHPALVLGAAAGTIVLKMLLIAPLARA
                     FGLSWTNGIRAGLLLGPGGEFGFVIVSVAIGEHLLAPETAGNVLFVTALTMAAIPLLS
                     ALGERLASRFTPKAAVDPSLLVPEVDALPRVIIAGFGRVGQMVAAMLEVHAVRYVAID
                     ADVDLVAGQRALGKPVYFGDLTQIRLLRRVHLDSARALVITLDDRQVADELVVAARAE
                     RADLLIIARARDAGHAAHLHSIGASEAVAETIEASLQLSEAVLVDVGVAMGPVIASIH
                     EKRAEMQAAIRTMAPGAARRTLGRRRLRDR"
     gene            4188..>4875
                     /locus_tag="VGC82_20590"
     CDS             4188..>4875
                     /locus_tag="VGC82_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY0425727.1"
                     /translation="MPTIPAERIGIDVGRKLPLEDAIDWAARNDVGILDIQLDTGANV
                     FTSFDQQRAHAIRRSCDHHDIRLGLHTLSAVNVAEYAPLVADAVDAYLKAYIEAAVLL
                     GAEWIVVHAGFHFSADIPARMRAALDRLNRAADYAASRSVLLLLENLNKEPPDAEVHY
                     LAHTIEEWRWFYEKLASPALQLSFTANHAHLVPEGVEGFLDAIPLPRVREVRLADCFR
                     NGHEQHLNPGQ"
BASE COUNT          952 a         1634 c         1516 g          773 t
ORIGIN      
        1 caccgtcggc tacgcattgc cggaagacat ggagcgcatg ttcatggcgg tccgcaaccg
       61 ggttccgggc atcgaaaaag tggtgctgtc cacgcacaac cataacgacc tcgggctggc
      121 ggtcgccaat accctggccg cgatccgggc cggcgtgcgc caggtcgaat ccaccatcaa
      181 cggcatcggc gaacgcgctg gcaacgcctc gctggaggag gtggtgatgg cgatccgcac
      241 ccgccaggat gcgatccccc gcgtcaaccg gatcaacacc cagagcctgc tgcgggtgtc
      301 gaagctgctc gcgaccatta ccggcttcga cgtgcagccg aacaaggcga tcgtcgggcg
      361 caacgctttc gcgcatgagg cggggatcca tcaggacggt gtcctgaagc atgccggcac
      421 ctatgagatc atgacgccgg aaagcgtcgg ctgggccaag aacagcctgg tgctgggcaa
      481 gcattccgga cgggcggcgt tccgtgacaa gctgaagacg ctggggtaca cgatcggcga
      541 caacaagctg aacgacgcgt tccgccgctt caaggacctg gccgaccgca agaaggtggt
      601 gttcgacgag gacatcgttg ccctggtgga cgacgaggtc atgcgcgacc acgagcgtat
      661 ccgcttcgtc tcgctcgacc tgcacgccgg gtcgaagggc cctgcccggg ctgagctgga
      721 actggaaatc gacggcgcga tgaaaatcgc cgcggcttcc ggcaacgggc cagtggatgc
      781 gaccttcaac gcgatccagg cgatcttccc gcacaccgcg aacctggtgc tgttctcggt
      841 tggcgcggtg accgagggaa ccgacgcgca ggcaaagacc accgtccggc tggaggaaaa
      901 cggcaagatg gtcgatggcc agggggcgga ttccgacacg atcgtcgctt cggcacgcgc
      961 ctatatccac gcgttgaaca agctgatggt caagcggctg cggacggagc ctgccgcgct
     1021 gtcggcctga tctcgtgggc ggtatcggtg ttcagaaaac gccggtaccg ccccggctac
     1081 gtgccttgca ggttccgccg gcggcttcgc ctagttgtag ccccagaacc cgctgcctcc
     1141 cgtcgtcggc cgattgcttg gcccatacga agaacctgtc gtcgatattc cgggcgtcgg
     1201 cgaggagtcg ggcgtatcgg ccgccggccc gaccggcagc ttctggattc ctggcccggt
     1261 gatgccgtag gaagcgggat cgccggaaca ggccgtcagg agaacaagca agccaagaac
     1321 tatcatcgcg cgcattgcat gaccttgctc caaccccgcg atatgtgtcc agcatcctga
     1381 cgacttccgt catgcgcctc gagtccccct gacattcctt ttggcctcgc gctgttttcg
     1441 tgccggcgac ctgaaccacg gccgaagtcc gctcgtatcg agaaccgggc cttggaggac
     1501 gagacgtgcg cttttgcctg cttgcggtat ggctggctgc cggcctgatc ggcggttgcg
     1561 cgcgcaagcc gcttgacacg gtattgggcg ggagcggtcc aaccttggac ccggttgcat
     1621 tcttcagcgg ccacacccat tccgggggcg tgatcgaaag ccggtccggc gcgccgaccg
     1681 agtggatcgt aaccgacagc ctcggcgcgc aggacgcgac cgggcggcta cggatggtgc
     1741 agcatctggc cttccaggac gggacgacgc aagagcgcac atggacgatg tggcgcagcg
     1801 gcccgcacct gttcatggct acagccaacg acatggtcgg ttcggcgaca ggccagtcga
     1861 acgggcgcat tttccactgg caatgggttc tggcacgggc acccggcaat gccctgagta
     1921 acgtgacgat ggaacaatgg atgtaccaga tggacgatga ttcgctgctt atccgtacaa
     1981 gtgtcagcaa gtttggattt atcctcgcgg aagtaacgga gcaattcaag cgcaccagga
     2041 aagactgacg tgagcggcgt catgctgttt gcctgacggc atgatccgcg cgcctacggc
     2101 gttcaccctc gctcgcgtac ccggcggtcg ggcgcgatga tgcttaaacg cccgctccga
     2161 agcaccgccc cgcgcgcgcc tcctcatttt atcgccaaat ccttcagcgg tcgcgcagcc
     2221 gcctgcggcc caatgtgcgt ctggccgcac ctggcgccat ggtcctgatc gccgcctgca
     2281 tctctgcccg cttctcatgg atcgaggcga tgaccggtcc catggccaca cccacatcga
     2341 ccagcaccgc ctcagaaagc tgcaagctgg cctcgatggt ttccgccacc gcctccgacg
     2401 caccgatact gtgcagatgc gcggcgtgcc ccgcatcccg agcccgcgcg atgatcagca
     2461 ggtctgcccg ttccgcccgc gctgcgacaa ccagttcgtc cgccacctgc cgatcgtcca
     2521 gtgtgatcac caaagcccgg gcggagtcca gatgcacgcg acgcagcaac cggatttgtg
     2581 tcaggtcgcc gaaatagaca ggtttgccca acgcccgctg gcccgcgaca agatcgacat
     2641 cggcgtcgat ggcgacatag cgaacagcgt ggacctccag cattgccgcc accatttgcc
     2701 caacccggcc aaatcccgcg atgatgaccc gcggcagcgc gtccacctcc ggcaccagca
     2761 gcgatggatc gaccgccgct ttcggagtga accgcgaggc cagacgctcc ccgagcgccg
     2821 acaacaaggg gatcgccgcc atcgtcaggg cggtaacgaa cagcacgttg cccgcggttt
     2881 cgggtgccag caggtgctca ccgatcgcta cggacacgat aacgaagccg aactcgccgc
     2941 ccgggccaag caacagtccc gctcggattc cgttcgtcca gctcaggccg aaggcccgcg
     3001 ccagcggggc gatgagcagc atcttcaaaa cgatcgtgcc ggccgcggcg ccaagcacca
     3061 gcgccggatg cgcgcctatg gtgcggatat ccaggctcat gccgatggac agcaggaaca
     3121 cgccgaggaa caggcctttg aacggcgcga tggtgacctc gacctgacgc cggtattcgg
     3181 ttccggccag cagcagcccc ccgatcagcg cgcccaaagg catggacagg ccggcggcgg
     3241 ccgttgccag gccggacgcg atgaccacaa gcaggcaggc ggcgacgaaa agttcctcgc
     3301 tgcgggtgcg cgcgacgcta cggaacagcg ggcgcaacac cacgcggcct agcaccacga
     3361 tcaccacgac cgccgcgaca gcccgtccga tagcgaggcc gaagccggcg ccgccgctat
     3421 cgtgcctcgc cgggtccagc gcgccgaggg cgaacagcgc cggtaccacg gccagatcct
     3481 ggaacagcaa aaccgcaaag ctcgctcggc cggtaggcgt gctcagccga tgttcgtccg
     3541 acagcacctg gatcacgacg gcggtggagg acattgccaa agtcaggcca agcaccagcg
     3601 cagcggtccg gtcgtatccc gagaggagcg ccgcaccgcc cacgactagt gcacaggccg
     3661 cgacctgcag gctaccaagg ccgaatacca gccggcgcat gacccacagg cgctcgaacg
     3721 acaactccag cccgatcatg aacagcaaca gcaccacgcc cagctgcgcg atcggttcga
     3781 tcgaggcgtt gtcgtggatg gtgatgacac cgagccaggg gaaatagccg gccaaggaac
     3841 caagaccgaa cgggccgacc accaggccga tcagcatgaa gccgagaacc gcactcacgc
     3901 gcaggcgatg gaacagcgga atgacgattc ccgctgcccc caggacaacc agggcttctt
     3961 tgtacagcga aaaatcgacg gtggcggatt ccatcgctga tgtttagcag attcgaacgg
     4021 aacaaggcgg gtcggccatt cgaggcgaaa cgcgggtcgc ggcgtttcag cacgaaccgt
     4081 ctgtgcaaag acgtggattg gcgtaggctt tccctgctcg caatcggggc cgcgaccgta
     4141 tccggatcgc cggtcccgac acctgaaacg accgggagca ccgtcttatg ccaaccattc
     4201 cagccgaacg catcgggatc gacgtgggcc gcaaactgcc attggaagac gcgatcgatt
     4261 gggctgcccg gaacgatgtc ggcatcctcg atatccagct cgacaccggc gccaacgtct
     4321 tcaccagctt cgatcaacag cgggcgcacg ccatccgccg atcctgcgac catcacgaca
     4381 tccgcctggg tttgcacacc ctgtcggccg tcaatgtcgc cgagtatgcg ccgctggtgg
     4441 cggacgccgt cgatgcctac ctgaaggcct acatcgaggc cgcggtgttg cttggtgccg
     4501 aatggatcgt cgtccacgcc ggatttcatt tctccgccga cataccggcg cgcatgcggg
     4561 ctgccctcga tcgcctcaat cgcgccgccg attacgccgc atccagatcc gttctgctgc
     4621 tgctggagaa cctcaacaag gagccgcccg acgccgaggt ccattatctt gctcacacga
     4681 tcgaggaatg gcgctggttc tacgagaaac tcgcctcacc tgccctgcag ctttctttca
     4741 ccgccaatca cgcgcacctg gtgccggagg gcgtcgaggg tttcctcgac gcgatcccgt
     4801 tgccccgcgt ccgcgaggtg cggcttgccg actgcttcag aaacggccat gaacagcacc
     4861 tgaatcctgg ccagg
//
LOCUS       DASWQN010001212         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_646550, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001212 DASWQN010000000
VERSION     DASWQN010001212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646550"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VGC82_20595"
     CDS             complement(<1..713)
                     /locus_tag="VGC82_20595"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0425728.1"
                     /translation="MNAPVDIRTRPADSRLGIIDCDIHPYPKAGALNAYLSKRWRKHL
                     IEYGKFNCGPFADRGTYPRFSPNTSRRDAWPPNGGPPGSDVDFIRRQLLDPYDIAYGV
                     LEPLLGGNTSRNLDEAAALCGAMNDWQAYEFVDPEPRLRASILIPSDDPDASVKEIEK
                     RAGDWRFAQIQLGSKTSEPLGRRRYWPIFAAAQANGMSIGLHIGGTQNSAPSAGGWPQ
                     FYLEDHHMLVHSMQNQAAS"
     gene            complement(710..1768)
                     /locus_tag="VGC82_20600"
     CDS             complement(710..1768)
                     /locus_tag="VGC82_20600"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0425729.1"
                     /translation="MIAGAIDCDIHPGVPDIKALLPYMNEFWQESFVSRGIDGFDMMS
                     YTLNAPITCRADWRVQGQRPGGSLEAMRSHALDRFGIGTAICNPLTGGQVAVSETMGA
                     AICSAVNDWVREHWLDQEPRLRASIVVPAQAPLLAAEEIDRCAGDRRFVQVLMPVACE
                     MMLGRSYYWPIWEAASRHGLPVGIHAGSMYRHAPTSTGWPSHYLHDYVAQSQTFEDQL
                     LSLISNGVFNKFPDLKFVLLESGVSWLPGFIWRAVKTWRGVRAEVPWVNRSPADIIRE
                     NIRLTVQPFDAPDREAVERIVEQIDADHMLLFASDYPHWQFDGDAVLPPGLSADLQRK
                     LRIDNPLATYPRLKEIMQ"
BASE COUNT          377 a          687 c          660 g          378 t
ORIGIN      
        1 atgctcgcag cctggttctg catggaatgg accagcatat gatgatcctc caggtagaat
       61 tgcggccagc cgccggcgga cggcgcactg ttctgcgtcc cgccgatatg caggccgatc
      121 gacatcccgt tcgcctgtgc cgcggcaaag atcggccagt agcgccgccg gcccagcggc
      181 tccgaggttt tcgagccaag ctggatctgc gcgaaacgcc agtcgccggc ccgcttctcg
      241 atctccttga ccgaggcatc cgggtcgtcg gaggggatca ggatcgatgc ccgcaacctg
      301 ggttcgggat cgacgaactc gtacgcctgc caatcgttca tcgcgccgca caacgccgcc
      361 gcctcatcca ggttgcgcga tgtgttgccg cccagcagcg gctccagcac cccataggcg
      421 atatcgtagg gatccagcag ttgccggcga atgaaatcca cgtccgatcc cggcggcccg
      481 ccgttcggtg gccatgcgtc gcgccgcgat gtgttcggcg agaaccgggg ataggtcccg
      541 cggtcggcga acgggccgca gttgaacttg ccatactcga tcaggtgctt ccgccagcgt
      601 ttcgacagat acgcgttcaa tgcacccgcc ttcggatacg gatgaatatc gcaatcgatg
      661 atgcccagcc ggctatccgc cggacgggtt cggatgtcga ccggagcgtt cattgcatga
      721 tctccttcag gcggggatag gttgccaacg ggttgtcgat gcgcagtttc cgttgcagat
      781 cggcggacaa gccaggcggc aggacggcgt cgccgtcgaa ctgccagtgc ggatagtccg
      841 aggcgaacag caacatgtgg tcggcgtcga tttgctcgac gatgcgttcc accgcttccc
      901 ggtccggcgc gtcgaacggc tgcaccgtca ggcgaatgtt ctcgcggatg atgtcggccg
      961 gcgaccggtt gacccatggg acttccgccc gcacgccgcg ccaggttttg accgctctcc
     1021 agatgaagcc cggcagccag gatacacccg attcaagcag cacgaatttc aggtcgggaa
     1081 acttgttgaa cacgccgttg gagatcaggc tcagcaactg atcctcgaag gtctgcgact
     1141 gggcgacgta atcgtgcaga taatgcgagg gccatccggt cgaggtcggg gcgtggcgat
     1201 acatgctgcc ggcatggatg ccgaccggca ggccgtgccg ggacgcagcc tcccagatcg
     1261 gccagtagta cgagcggccc agcatcattt cgcacgccac cggcatcagg acctggacga
     1321 agcgccggtc gcccgcgcaa cggtcgattt cctcggctgc cagcagcggc gcctgcgccg
     1381 gcacgacgat ggacgcgcgc aggcgcggct cctggtccag ccaatgctcc cgcacccagt
     1441 cgttgaccgc cgaacagatg gccgcaccca tggtttcgct gaccgccacc tggccgccgg
     1501 tcaacggatt gcatatcgcc gtaccgatgc cgaaccggtc cagcgcatgg ctgcgcatcg
     1561 cctccaggct gccaccgggg cgctggcctt gaacccgcca gtccgctcgg caggtgatgg
     1621 gggcattcag cgtgtaggac atcatgtcga agccgtcgat accacgcgag acgaacgatt
     1681 cctgccagaa ctcgttcatg tagggcagca gggccttgat gtccggcacc ccgggatgga
     1741 tgtcgcagtc gatcgcgccg gcgatcattg agctactcct gtgcctgcac ggcaagatga
     1801 ggctgatatc aacagcccga agggttggtg tggcggcttc gcgccaaatc aatagcatag
     1861 cgatcgccgc tgacccggca acgggtcaac ttttagggcc gttggtatga caatgcgagc
     1921 ggcaggtttt cgctgtcaaa ctcatgttgg cttcacccct gcccgccgca gcgtccgttg
     1981 ccagtcggcc gctaccccgt ccagcgcagc cttcgccgac tcctgcccga gcagcacctt
     2041 ggatatgccg ctgcgcaact gcagttccat cgacggccag aatggctccc gcggttcaag
     2101 ct
//
LOCUS       DASWQN010001213         5926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1569069, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001213 DASWQN010000000
VERSION     DASWQN010001213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569069"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..983)
                     /gene="lpxA"
                     /locus_tag="VGC82_20605"
     CDS             complement(168..983)
                     /gene="lpxA"
                     /locus_tag="VGC82_20605"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY0425730.1"
                     /translation="MTNTRPHTIDRTSIHPTAIVAHGAELGPGVEIGPFCSVGANVVI
                     EAGAKLLSHVVIEGHTRIGEGAQLYPFCTVGLAPQDMKYKNEPTRCEIGARTLVREHC
                     TIHRGTATGVGVTRIGADCMLMAVVHVAHDCQLGDSVIVANNAVMGGHVTIGDHAVVG
                     GAAAIHQFVRIGRAAMIGGVSGVEADVIPFGSVIGNRARLAALNIIGLRRRGFDKAAI
                     QRLHIAFRALYSKQGVFAQRLAATRARFGDDPLVAEILAFIDAPSHRGLIKAE"
     gene            complement(1222..1818)
                     /gene="fabZ"
                     /locus_tag="VGC82_20610"
     CDS             complement(1222..1818)
                     /gene="fabZ"
                     /locus_tag="VGC82_20610"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY0425731.1"
                     /translation="MHQTGTPPLDNLEPDKIAPDKITPGHRAPGDVTPDAQATASAPE
                     TGKNIDVIDIERIMHAIPHRYPFLLLDRMVDVVVGISAVGVKNVSVNENFFAGHFPNH
                     PVMPGVLIIECMAQTAAVLVVETLGSGAAGKVVYFMSIEGAKFRRPVVPGDQLRIHIV
                     KQRHRGNVWKFHGIARVDGVAVAEATYSAMIMDHAPGS"
     gene            complement(1824..2933)
                     /gene="lpxD"
                     /locus_tag="VGC82_20615"
     CDS             complement(1824..2933)
                     /gene="lpxD"
                     /locus_tag="VGC82_20615"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY0425732.1"
                     /translation="MAPGADRSMLAGDPRFFRRAGPHTLAAVVDAATVNGERAEAPPR
                     RLMLHRIAPLAAATGEEVAFCLNNRKYLPALAATKAAAVIVHPAMRDHVPDTAVAILT
                     SEPLVAWANVAALFHPLPPVVPGIHPSAIVAASARIDPATEVGPLAVIGENVTIGPRG
                     RIGPMVVIGDGVQIGRDVRIGPHASLSHALVGDRVYIYPGARIGQEGFGFAITPEGFH
                     TVPQLGRVLIEDDVEIGANTTVDRGTMEDTIIGAGSRIDNLVQIAHNVRIGRACVIVA
                     LVGVSGSAVLEDQVVLAGQAGVAGHIRIGTKSRIGAQAGVMADVPPRSDLVGAPAQPV
                     KAFFREVATIRRWVRNGGIPHRDTARATKPNPDGD"
     gene            complement(2936..3859)
                     /locus_tag="VGC82_20620"
     CDS             complement(2936..3859)
                     /locus_tag="VGC82_20620"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY0425733.1"
                     /translation="MSKQSIRCANGLKSIGAVVALALAAYPASGFAQQSQSGEWFVPG
                     QARPTASPSRQAPRQTQQSPGPSSGPGALPLPPDPAADQAGGPPPLQVQLPPAPEIPA
                     IPKGQATPAAIVGILSVPDVLRVSTAYQAAYKELNARGQKLNEDAQKEQGALRDLGQS
                     FAAERAKLPPEQVRNKEKEIQERATEAQRRFGERNRIIQEAGQYVMMQINRTMEQVAQ
                     QVALSRGINLVLNRAQILGTTADFDLTPAVADVLNKALPSVVLPPDGVSPLAMAGPAE
                     PAQGGAPGAPAGASSTPASATAAPAKSPQKR"
     gene            complement(3859..>5926)
                     /gene="bamA"
                     /locus_tag="VGC82_20625"
     CDS             complement(3859..>5926)
                     /gene="bamA"
                     /locus_tag="VGC82_20625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY0425734.1"
                     /translation="DSLVVRVVENPVVNRVAYEGNHKLTDDLLRPELQLRPRAVYTAA
                     LAEADRQRILGLYAKRGYYDATVEPKIIRLDQNRVDVVFQINDGSATLVSKIVINGNK
                     AFSEDRLSEVINTREARWWRFLSTSDQYDPERLAFDKELLRRFYLKNGYADYQLVAAS
                     AELLPDRKAFVLSFTVNEGARYRVGNVVIKSQLRNLSEKDLQPSLQLQSGDWYDGDAV
                     GRSADLIEEAVHDRGYAFVDVKPRVARDPEKHIVDLSFDVGEGPRVYVERIDIVGNTR
                     TKDKVIRREFRLAEGDPYSAEAIRRSKTRLTDLGYFQGVDVVTNPGSAPDKAVLTTNI
                     AEKATGELSFGGGYSTDAGALVDIGLAERNLVGTGINASINGVLAQRRSSVSASITDP
                     YFLDRNLIAGLDIFLIQTNFLGTEPYDERREGFALRLGYAFNEHLRQVWSYSLVNRDV
                     FNVQLGASPFIRNEAGTTLLSQISQVLTLDYRDSRIYPHSGYVVEGGTDFAGLGGDAR
                     FIRGNLNAAYYIPLEHLLGNPDWGIKIGGSTGYMWLLPGGREEIIDRFFLGGENLRGF
                     QTGGAGPHDVISGDPLGGRFIWTQSTELRFPLPVSPDLGLNGRAFVDVGSLTDASFRN
                     SNACQTSATVSCQIAASGSPRLGAGFGISWRTAFGLINVDLAPFVIKQPHDQTQIFRF
                     GFGTRF"
BASE COUNT         1009 a         1847 c         1997 g         1073 t
ORIGIN      
        1 ctgccaacgg ttttccaggc gataaagcgc gcgtatgccg gggtcggcta ctctggtggc
       61 ggtcggtaat gcgtcgctca tttttcttgg ctcccggcgg ttcctgtggc gcaggatcgc
      121 agccgggatg gcgacgggca agcggggccg cggagcgagg ggtttggcta ctccgccttg
      181 atcaagccgc ggtggcttgg tgcgtcgatg aacgccagga tctcggccac cagtggatca
      241 tcgccgaacc tcgcgcgggt cgcggccagc cgctgcgcga acacgccttg cttgctgtag
      301 agcgcgcgga acgcgatatg cagccgctgg atcgccgcct tgtcgaagcc gcggcggcgc
      361 aggccgatga tgttgagcgc ggccaggcgt gcgcggttgc cgatgacgct gccaaacgga
      421 atgacgtccg cctcgacccc cgagacgccg ccgatcatgg ccgctcggcc gatgcggacg
      481 aactggtgga tcgccgccgc gccgccaacc acggcgtggt cgccgatagt gacgtgaccg
      541 cccatgacag cgttgttcgc cacgatgacg ctgtcgccga gctggcagtc atgcgcgacg
      601 tgaaccacgg ccatcagcat gcagtcggcg ccgatccggg tcacgccgac gccggtggcg
      661 gtgccgcgat ggatggtgca gtgctcccgc accagggtac gggcgccgat ctcgcagcgt
      721 gtcggctcgt ttttgtattt catgtcctgc ggcgccaggc cgacggtgca gaacggatac
      781 aactgggcac cctcgccgat gcgtgtgtga ccctcgatca cgacgtgcga cagcagcttc
      841 gcgccggcct cgatgacgac gttggcaccc accgagcaga acggcccgat ctctacgccg
      901 gggccgagtt cggcgccatg cgccacgatc gcggtcgggt gaatgctggt ccgatcgatc
      961 gtgtgcggcc tggtattcgt catcaaatcc ataatcgttc cgttcaagga aagcggtttc
     1021 gcggcaggca atagcgaaac tattgtcgga agcgccgacc accaacacaa tgattgttgc
     1081 ccgatgtttc cggccatgcc tgtaacgccc tgtttcgcat ggcattttgg cgttactaag
     1141 gcaggaaagg cctcaggttg aagccgtcag accctgggtt gaaccaacct caaaagatcg
     1201 cgctggaagt tagacctccg gtcacgatcc cggggcgtgg tccatgatca tcgccgagta
     1261 ggtcgcttcg gcgacggcaa cgccatcgac cctggcgatc ccatggaact tccagacatt
     1321 gccgcggtgg cgctgtttga cgatgtggat gcgcagttgg tcgccgggga caacagggcg
     1381 gcggaacttg gcgccctcga tcgacatgaa atagaccacc ttgccggcgg caccggatcc
     1441 cagcgtttcc accacgagta cggcggcggt ctgcgccatg cactcgataa tcagcacccc
     1501 cggcatcacc ggatgattgg gaaaatggcc ggcgaagaag ttctcgttca ccgacacatt
     1561 cttaacgccg acggcggaga tgcccactac gacgtccacc atccgatcca gcagcaggaa
     1621 cgggtagcga tgcgggatgg cgtgcatgat gcgctcgata tcgatcacat cgatgttctt
     1681 gcctgtttcg ggtgccgagg ctgttgcctg cgcgtccggc gtcacatcgc cgggcgccct
     1741 gtggcctggc gtgatcttgt cgggtgcgat cttgtcgggt tccagattat cgagtggggg
     1801 agtgccggtt tggtgcatga tcttcaatcc ccatcggggt tgggttttgt agcgcgcgca
     1861 gtgtcccggt ggggtatgcc gccgttgcgg acccagcgcc ggatggtcgc cacctccctg
     1921 aagaaagcct tcaccggctg agccggcgcg cccaccaagt cggagcgcgg cggcacgtcg
     1981 gccatgacgc cggcttgtgc gccgatgcgc gatttggtgc caatgcggat atggcccgcg
     2041 acgccggcct ggccagccag cacgacctga tcttcgagca ccgcagaacc ggatacgccg
     2101 accaaggcga cgatgacgca ggcgcgccca atgcggacgt tgtgggcgat ctggaccagg
     2161 ttgtcgatgc gcgatccggc gccgatgatg gtatcctcca tggtgccgcg atcaacggtg
     2221 gtgttggcgc cgatctcgac gtcgtcctcg atcagcacgc ggccgagttg aggcaccgtg
     2281 tggaagcctt ccggggtgat cgcgaagccg aatccctctt ggccgatgcg agcgccggga
     2341 tagatataca cgcgatcgcc gaccagggcg tgggacagcg acgcatgcgg accgatgcgc
     2401 acgtcgcggc cgatctgcac gccgtcgccg atgaccacca tgggcccgat gcggccgcgc
     2461 ggaccgatcg tcacgttttc tccgatgacc gcgagcgggc caacctcggt ggccggatcg
     2521 atgcgagcgg aagcggcgac gatcgccgat ggatggatgc cgggaacgac cggcggcagc
     2581 ggatggaaca gggctgccac atttgcccag gcgaccaggg gttcggaggt caggatcgcc
     2641 accgcggtat ccggcacatg gtcgcgcatg gcgggatgga cgatgaccgc cgcggccttg
     2701 gtcgcagcca gtgccggcag gtatttccgg ttgttcagac agaaggcgac ctcttcaccg
     2761 gtggccgcgg ccagtggtgc gatgcgatgc agcatcaggc ggcgcggcgg cgcctcggcc
     2821 cgttcgccgt tgaccgtcgc ggcatccacc acggcggcca gcgtatgcgg gccggcccgc
     2881 cgaaagaagc ggggatcgcc cgccaacatc gagcggtccg cgcctggtgc cattgttagc
     2941 gcttctgggg cgacttggct ggcgcggccg tcgcgctcgc cggggtcgag gacgcgcccg
     3001 ccggagcacc gggcgcacca ccttgggcgg gttcggcggg gccggccatc gccagcgggg
     3061 atacgccgtc cggtggcagg accacgctgg gcagcgcctt gttcagcacg tcggccacgg
     3121 ccggcgtcag gtcgaaatcg gccgtcgtgc cgaggatctg ggcgcggttc agcaccagat
     3181 tgatgccacg cgacagtgcc acctgctgcg ccacctgctc catggtgcgg ttgatctgca
     3241 tcatgacgta ttgaccggct tcctggataa tccggttacg ctcgccgaag cggcgctgcg
     3301 cctcggtcgc gcgttcctga atttccttct ccttgttccg cacctgctcg ggcggcagct
     3361 tggcccgttc ggcggcgaag gactggccca gatcgcgcag cgcgccctgt tccttctgtg
     3421 cgtcctcgtt cagcttctgg ccgcgcgcgt tcagttcctt gtatgccgcc tgatacgccg
     3481 tggacacgcg caggacatcg ggcaccgaca ggatgccgac aatcgccgcg ggggtggcct
     3541 gtcccttcgg tatcgccggg atttccggtg ccggcggaag ctggacctga agcggaggcg
     3601 gaccgccggc ctgatcggcg gccggatcgg gcggcaacgg cagcgcgccc ggcccgctgg
     3661 aggggcccgg agactgctgg gtttgcctcg gcgcctggcg ggaaggcgag gcggtgggac
     3721 gagcctgacc tggcacgaac cattcgccgc tttgcgattg ctgggcgaaa cccgaagctg
     3781 gataggccgc aagcgccagc gcgacgaccg cgccgatact ctttaacccg ttggcacacc
     3841 tgatggactg tttactcact agaacctcgt accgaagcca aaccggaaga tttgtgtctg
     3901 gtcgtgcggt tgcttgatca caaacggcgc caagtcaacg tttattagac caaacgccgt
     3961 tcgccacgag atgccaaatc cggcgccaag acgaggcgaa ccggaggcgg cgatctggca
     4021 ggagacggtg gcggaggttt ggcacgcatt ggagtttctg aaacttgcgt cggtcagcga
     4081 tccgacatcg acgaacgctc ggccgttcag gcccaggtcg ggggaaaccg gcaacggaaa
     4141 gcgcagttcc gtgctctggg tccagatgaa gcgtccaccg agcggatctc cggatatgac
     4201 gtcatgcggg cccgcaccac cggtctggaa gccgcgcaga ttctcgccac ccaggaagaa
     4261 cctgtcgatg atttcctcgc gacctcccgg cagcagccac atatagcccg tgctgccgcc
     4321 gatcttgatg ccccagtccg gattgcccag caggtgctcc agcgggatgt agtaggcagc
     4381 gttcaggttg ccgcggatga accgtgcgtc gccgccaaga cccgcgaaat cggtgccgcc
     4441 ctccaccacg taaccggaat gcgggtaaat ccggctgtcg cggtagtcca gcgtcaggac
     4501 ctggctgatc tgggacagca gcgtggttcc cgcctcgttt cgaataaacg ggctagcgcc
     4561 tagttggacg ttgaagacgt ctcgattgac gagcgagtag ctccagacct gccgcagatg
     4621 ctcgttgaag gcgtagccga ggcgcagggc gaacccttcg cgccgttcgt catacggttc
     4681 cgtccccagg aagttcgttt ggatcaggaa gatgtcgagg ccggcgatca ggttgcgatc
     4741 caggaaataa gggtcggtga tggaggcgct gaccgaactg cgcctttgcg cgagcacgcc
     4801 gttgatgctg gcattgatgc cggtgccgac caggttgcgc tcagccaggc cgatatccac
     4861 cagtgcgccc gcatcggtgg agtaaccgcc gccgaacgac aactcgccgg tggctttttc
     4921 ggcgatgttc gtcgtaagca cggccttgtc gggggcagaa cctgggttgg tgacgacgtc
     4981 aacgccctgg aaatatccga ggtccgtcaa ccgggttttg gagcggcgga tcgcttcggc
     5041 gctgtacgga tcaccttcgg cgaggcgaaa ctcccgccgg atcaccttgt ccttggtgcg
     5101 ggtgttgccg acgatgtcga tgcgctcgac atagacgcgc ggcccctcgc cgacgtcgaa
     5161 gctcaggtca acgatatgct tctcggggtc gcgggccacc cgcggcttga cgtccacaaa
     5221 ggcataaccg cggtcgtgaa ccgcctcctc gatcaggtcg gcgctgcgcc cgacggcatc
     5281 gccatcgtac cagtcgccgg attgcagttg caggctgggc tgcaggtcct tctcgctaag
     5341 attgcgcaac tgcgacttga tgacgacgtt gccgacccgg tagcgggcac cctcgttcac
     5401 ggtgaacgac aagacgaacg ccttgcggtc cggtagcagt tcggcgctgg cggccacgag
     5461 ctggtaatcg gcatagccgt tcttcagata gaagcggcgt agcagttcct tgtcgaaggc
     5521 caatcgctcc ggatcatact ggtcggaggt ggacaggaat cgccaccagc gggcttcgcg
     5581 ggtgttgatg acttccgaca ggcgatcctc gctgaaggcc ttgttgccgt tgatcacgat
     5641 cttgctgacc agcgtggcgg aaccgtcgtt gatctggaac accacatcga cgcggttctg
     5701 gtccagacgg atgatcttgg gctcgacggt ggcatcgtag tagccgcgct tggcgtacag
     5761 gccaaggatg cgctggcggt cggcctcggc caaagcggca gtgtacacgg cgcggggccg
     5821 aagctgcagt tcgggccgga gcaggtcatc ggtgagcttg tggttgcctt cgtaggcgac
     5881 gcggttcacc accgggtttt cgaccacgcg caccaccagt gagtcg
//
LOCUS       DASWQN010001214         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2493453, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001214 DASWQN010000000
VERSION     DASWQN010001214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493453"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VGC82_20630"
     CDS             <1..145
                     /locus_tag="VGC82_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007282649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0425735.1"
                     /translation="QAVHMIADGDFIGPAKNGDPKTSRLVFIGRNLNRPQLRRGFESC
                     AAD"
     gene            206..1252
                     /locus_tag="VGC82_20635"
     CDS             206..1252
                     /locus_tag="VGC82_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY0425736.1"
                     /translation="MSQSTRADFLLQTRGTSRELDAFVVAARFSRDGSRGGFALGDGS
                     VRLVGVGTPDDWTSVTAHDGAVLDFAADPAGDGFVAGGDDGKLNRIGGDGTVANISDF
                     GSKWVEHVATCALDKGKGLIAASAGRSVRLFDQSGQLLKELTHPSTVTGLVFDGKGKR
                     IGASHYNGASLWFVAAKTDTPRKLEWKGSHTGILIHPEGEAVVTAMQENSLHGWKLPN
                     GEHMRMSGYPAKTNSLSFTRNGKWMASSGADSMVLWPFFGGGPMGKAPLELAGGDGII
                     CTQVAANPNNEIVAGGFADGLVVVADITSSRILPVVPPDHGPISTLAWSPDGTQLAVG
                     TETGFAALVDFTRR"
     gene            complement(1259..>1702)
                     /locus_tag="VGC82_20640"
     CDS             complement(1259..>1702)
                     /locus_tag="VGC82_20640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY0425737.1"
                     /translation="RRSIALNEFAPLADLPGIHLVSLQKHGTEQAWTCPPDMTLIDPM
                     ADVNDFAGTAALIANLDLVIAIDTSVAHLAAGMGRPVWLMSRYDGCWRWLHDREDSPW
                     YPGMRIYRQQRPHDWPAVIARIRNHLAHATHDFLCSHDRTLFRSA"
BASE COUNT          315 a          511 c          588 g          288 t
ORIGIN      
        1 ccaggcggtg cacatgatcg ccgacgggga tttcatcggt ccggcaaaga acggcgatcc
       61 gaagaccagc cgcctggttt tcatcggccg caacctgaac cgcccgcagt tgcggcgtgg
      121 gttcgagagc tgcgcggcgg attgagctgg gggattgcca gccaggccat gaccttactc
      181 aggccatgac gaaagaacca ccatcatgag ccaatccacc cgggccgact ttctgttgca
      241 aacccgcgga acctcccgcg agctggacgc ctttgtcgtc gccgcgcggt tttcccgtga
      301 cggaagccgc ggcggcttcg ccctcggcga cggttcggtg cgactggtgg gcgtagggac
      361 gccggacgac tggacatccg ttaccgccca tgacggtgcc gtgctggact tcgccgccga
      421 tcccgcgggc gacggcttcg ttgcgggcgg cgacgacggg aaactgaacc gcatcggcgg
      481 cgacggcacg gtcgccaata tcagcgactt cggatcgaaa tgggtggagc acgtcgccac
      541 ctgcgcgctg gacaaaggca agggactgat cgccgcgagt gccggcagat cggtcaggtt
      601 gttcgaccaa tccggccagt tgctgaagga attgacccac ccctcgacgg tcaccggcct
      661 ggtgttcgat ggcaagggca agcgcatcgg tgcctcgcac tacaatggcg cctcactgtg
      721 gttcgtcgcg gcgaagacgg atacgccgcg aaagctggag tggaagggca gccataccgg
      781 catcctgatc catccggaag gcgaggcggt ggtgacggcg atgcaggaga attcgctgca
      841 cggctggaaa ctgccgaatg gcgagcacat gcgcatgagc ggctacccgg ccaagaccaa
      901 cagcctgtcg ttcacgcgca acggcaaatg gatggccagc agcggcgccg actcgatggt
      961 gctatggcca ttcttcggcg gcggcccgat ggggaaggcg ccgttggaac tcgccggcgg
     1021 cgacggcatt atttgcaccc aggtcgcggc gaacccgaac aacgagatcg tggcgggcgg
     1081 tttcgctgac ggtctggtgg tggtggcgga cattacgtcg tcgcgcattt tgccggtcgt
     1141 gccgcctgac catggtccga tcagtacgct ggcatggagc ccggacggga cccagttggc
     1201 agtgggcacc gaaaccgggt tcgctgcgct ggtggacttc accaggcgct agatcggttc
     1261 acgctgaccg gaacagggtt ctgtcgtggc tgcaaaggaa gtcgtgagtg gcatgcgcga
     1321 gatgatttcg gatgcgggcg atcacggcgg gccagtcgtg cgggcgctgt tggcgataga
     1381 tgcgcatgcc cggataccag gggctgtcct cgcggtcatg cagccatcgc cagcagccgt
     1441 cgtagcgtga catcagccaa acgggccgac ccatgccggc ggccagatgc gcgaccgatg
     1501 tatcgatcgc gataaccaga tccagattgg cgatcaacgc cgcggtgccc gcgaagtcgt
     1561 ttacgtcggc catcgggtcg attagcgtca tatcaggcgg acaggtccac gcctgttcag
     1621 tgccatgctt ctgcaggctg accaggtgga tgccggggag atccgccagc ggggcgaatt
     1681 cattcaaagc gatcgagcgg cg
//
LOCUS       DASWQN010001215         2799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2099274, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001215 DASWQN010000000
VERSION     DASWQN010001215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099274"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1727)
                     /locus_tag="VGC82_20645"
     CDS             complement(129..1727)
                     /locus_tag="VGC82_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEY0425738.1"
                     /translation="MRDFQLPGRSPVYATHGMAATSKPASTLTALDVLRAGGNALDAA
                     VAAVAVQCVIEPQSTGIGGDCFCLYAPAGTGKVYALNGSGRAPAAANMDWYESHQIRA
                     LENTSAHSVTVPGAVNAWETLLKAHGRKGLDELLQPAIRFAEEGWPVHPKVSWDWGRS
                     VEKLRKGGSTPFLPNGAAPKVGDLFTQPALAETLRMISRHGAKAFYEGPVAADIVATL
                     RERGGLHTEEDFAKGLHNAEFVEPITLNWKGYDVYQCPPNGQGLLVLMILGMLGGLPS
                     APDGASGVVRAHRHIEAARLAYRDRDAFVADPGMVDVPVKKLLSPDYLASQRALINDT
                     AAMRNLPAAGESMLPPHKDTVYLCVVDKDGNACSFINSLFENFGSGILAHKSGVMLQN
                     RGFGFRLERGHPNCIAGGKRPLHTIIPGMLMKDGRAVMPFGVMGGHYQPMGQSWFLTN
                     FLEYGLDIQQSIDQFRMFPYGGKLQVERGIPEELVRKLAAMGHQPEPTERPLGGGQAI
                     WIDHDRGVLVGGSEPRKDGLALGY"
     gene            1798..2136
                     /locus_tag="VGC82_20650"
     CDS             1798..2136
                     /locus_tag="VGC82_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEY0425739.1"
                     /translation="MDEITYADFEAVDIRVGTIITAQPFPEARKPSIKLTIDFGPEIG
                     IKKSSAQLTVHYKPDQLIGRQVCAVVNFPPRQIGPFISEVLTLGMPDEDGAVVLVRPD
                     LRVPNGGNLF"
     gene            2265..2714
                     /locus_tag="VGC82_20655"
     CDS             2265..2714
                     /locus_tag="VGC82_20655"
                     /inference="COORDINATES: protein motif:HMM:NF013853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HEY0425740.1"
                     /translation="MYQDDIVSWSERQAQLLRQHAAVARVNDAFDWPNIVEEIGAVGG
                     SERAALASHIGTVIEHLLTLQASPAKEPRSGWRETVIRARSAIAGQLEDSPSLRPKVD
                     SIVARQLSRQRRLVADVLALYNQAPLVPLEGTTFSVAQVLDPWFPAD"
BASE COUNT          540 a          892 c          865 g          502 t
ORIGIN      
        1 cgcgttgacg gcatggtcca cgtcctcgat ccgcccgatg cagctttcca gcagctcaag
       61 cgacgaaagc tgccgggatc cgatcagccg ccgagcttcg acggccgaca ggtcgcaagg
      121 ttccaccgtc agtaaccgag cgcgaggccg tctttccgag gttccgatcc gcccaccagg
      181 actccgcgat catggtcgat ccagatcgcc tgtccgccgc caagcggacg ttccgtcggc
      241 tccggctggt ggcccatggc ggccagcttg cgcaccagct cctcgggaat gccgcgctcg
      301 acctgcaatt ttccgccgta cgggaacatc cggaactgat cgatcgactg ctggatatcc
      361 agcccgtatt ccaggaaatt cgtcaggaac caggactgtc ccatcggctg gtagtgcccg
      421 cccatcacgc cgaacggcat caccgcgcgc ccgtccttca tcagcatgcc cggaatgatc
      481 gtgtgcagcg ggcgcttgcc gccggcgatg cagttcggat gtcctcgctc cagccggaaa
      541 ccgaaaccgc gattctgcag catgacgccc gatttgtgcg ccaggatgcc gctgccgaaa
      601 ttctcgaaca gcgagttgat gaaactgcac gcgttgccgt ccttatcgac gacgcagaga
      661 tacaccgtgt ccttatgcgg cggcagcatg gactcgccgg ccgccggtag gttccgcatc
      721 gccgcggtat cgttgatcag cgcccgctgc gacgccagat agtccgggct gagaagcttc
      781 ttgaccggaa catcgaccat gccgggatcg gccacgaagg cgtcgcggtc gcggtaggca
      841 agccgagctg cctcgatatg gcgatgggcg cgaacaacgc cggaagcacc gtcgggcgcg
      901 gacggcaacc cgcccagcat acccaggatc atgagcacca gcagaccctg gccattgggc
      961 gggcactggt aaacgtcgta gcctttccag ttcagcgtga tcggctcgac gaactcggca
     1021 ttgtgcaggc ctttggcgaa atcttcctcg gtatgcagcc cgcctcgctc gcgtaacgtc
     1081 gcgacgatgt cggcggcgac cggaccctca tagaaagcct tggcgccgtg gcgggagatc
     1141 atgcgcaagg tttcggccaa ggccggctgc gtgaacaggt caccaacctt gggtgctgct
     1201 ccgttcggca ggaacggggt tgatccgccc ttgcgcagtt tctcgacgct gcgtccccag
     1261 tcccacgaga ctttcggatg caccggccag ccttcctcgg cgaaacgaat ggccggctgc
     1321 agcaactcgt cgaggccctt gcggccgtgc gctttcagca gcgtttccca ggcattgacc
     1381 gcgcccggca ccgtcacgga atgcgccgag gtattttcca acgcccttat ctgatgactt
     1441 tcataccaat ccatgttggc ggcggcaggc gcccgcccgg acccgttcag cgcatagact
     1501 ttgccggttc cagccggggc gtacagacag aaacagtcac cgccgatgcc ggtggattgc
     1561 ggctcgatca cgcattgcac cgccaccgcg gcgacggcgg cgtccagcgc attgccccca
     1621 gcacgcagca catccaatgc cgtgagggtc gaggcgggct ttgacgttgc cgccatcccg
     1681 tgagtggcat aaaccgggct acggccagga agctggaagt cgcgcatggt ggtcctcggg
     1741 tcgagaagcc atcactctat cccagccaat cgcagaggcc aacgcgggag gaacagcatg
     1801 gatgaaatca cctacgccga cttcgaggcg gtggacattc gcgtcggcac catcatcacc
     1861 gcccaaccct ttccagaggc gcgcaagccg tcgatcaagc tgacgatcga tttcggcccc
     1921 gagatcggga taaaaaaatc gagcgcccag cttaccgttc actacaagcc ggatcagttg
     1981 atcgggcgcc aggtgtgcgc cgtggtgaac ttcccgccgc gccagatcgg accgttcatc
     2041 agcgaggtat tgacgctggg gatgccggac gaggacggtg cggtcgtgct ggtccggccg
     2101 gaccttcggg tgccgaatgg aggcaacttg ttctgacggg caagataagg agaatgcaac
     2161 cgtgctgtgg ccggtcgcac cggttggatt gctggtttgg acgagaactt gcgtgatcgg
     2221 atcgaacagc tgccatgtgg cggcggagat acggaatgag tgacttgtat caggacgaca
     2281 tcgtatcgtg gagcgaacgc caagcgcaac tgctgcgcca gcatgcagct gtcgcgcgag
     2341 tcaacgatgc attcgattgg cccaacatcg ttgaggaaat cggagctgtg ggcggttcag
     2401 aacgcgcggc actggcgagc cacatcggaa ccgtcatcga acatctgttg acgctgcaag
     2461 cttccccggc aaaggagccg cgcagcggtt ggcgagaaac cgtaatccgc gcccgctccg
     2521 cgattgcggg acagctggaa gacagtccaa gtctgcgtcc aaaggtcgat tctatcgttg
     2581 cccgccagct ttcgcggcag cgccgattgg tggcggatgt tttggcgctg tataaccaag
     2641 cgccgcttgt accgctggaa ggaacaacct tcagcgtcgc gcaggtgctc gatccgtggt
     2701 tcccggccga ttagtcgccg ccgagtagga gtgttacccc acctgataaa accccggacc
     2761 atccggcggt tcggcaagat cctcgtcgat cgacgacac
//
LOCUS       DASWQN010001216         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1439589, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001216 DASWQN010000000
VERSION     DASWQN010001216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439589"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..976
                     /locus_tag="VGC82_20660"
     CDS             131..976
                     /locus_tag="VGC82_20660"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0425741.1"
                     /translation="MATDRISNKRPWTFYALGTLFGAYLLFLYGPMFVIYVLSFQGEN
                     GGVTFPMVGFSLTWFQGIFQKGQMANIPVSFSRSMALAAVVSSLTVLISVAAGLGFRR
                     RFPGSGALFYLAVASLVMPSLLVGFGIGLGFQFLGIEPSLFTSALGAQLTWTLPFGLF
                     AMFAVVNRFNRAWEEAATDLGASAWQRLRYITIPILLPGIIGVAMGAFTLSYDEYART
                     TLTIGQKNTLPLEIYALISSATSPTLFAIGSLTTGLSFLAIFIALLLVLRLQRRRALV
                     AVKVG"
     gene            976..>1560
                     /locus_tag="VGC82_20665"
     CDS             976..>1560
                     /locus_tag="VGC82_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425742.1"
                     /translation="MDSRFDVELISVTKRYGTTLAVDNISLRIPRGTYCCLLGPSGCG
                     KTTTLRMIAGHEAVTEGDILIHGENVSDLPPIKRGTAMMFQSYALFPHMSCLDNVAFS
                     LKMRGVAKKERHAQAREYLTLVHMTDYADRLPAQLSGGQQQRIALARALINRPRVLLL
                     DEPLSALDPFLRVKVREELKRLQRELGITFIHVTH"
BASE COUNT          255 a          474 c          489 g          342 t
ORIGIN      
        1 ccaccgagat ccaggcgatg cagtacccgc cggccgccgc caatgcgatg gtgctggtca
       61 tcttcgtcgc tattctggtg gctgtgatga tgaaggtggt cgatgttcgc aaggaactgg
      121 tgaaatagcg atggcaaccg accgtatcag caacaaacga ccttggacgt tctatgcgct
      181 ggggaccttg ttcggggcgt acctgctgtt cctttatggc ccgatgttcg tcatctacgt
      241 gctgtccttc cagggcgaga acggcggcgt gacgttcccg atggttggtt tctcgctgac
      301 ctggttccag ggaatattcc agaaaggcca gatggcgaac atcccggtat cgttctcgcg
      361 gtcgatggcg ctcgccgcgg tggtcagcag ccttacggtg ctgatttcgg ttgccgccgg
      421 ccttgggttt cgccggcgtt tccccgggtc cggcgcgctg ttctacctcg cggtcgccag
      481 cctggtgatg ccaagcctgc tggtgggttt tgggatcggg ctgggtttcc agttcctggg
      541 gatcgagcca agcctgttca cctcggcgct tggggcgcaa ctgacgtgga ccttgccgtt
      601 cggcctgttc gcgatgttcg cggtggtcaa ccgcttcaat cgagcgtggg aggaagccgc
      661 gaccgacctt ggcgccagtg cctggcagcg gctgcgctat atcaccatcc cgatcctgtt
      721 gccgggcatc atcggtgttg ccatgggcgc gttcaccctg tcctatgacg agtatgctcg
      781 aacgaccctg acgatcggtc agaagaatac gctgccgttg gaaatctacg ctttgatctc
      841 ttccgcgact tcgccgacgt tgttcgcgat cggcagcctg accaccgggt tgtcgttttt
      901 ggcgattttc attgcactgc tgctcgttct gcgcctgcag cggcggcgcg ctctcgttgc
      961 agtaaaggtt ggatgatgga cagtcgcttc gacgtcgagc ttatcagcgt caccaagcgt
     1021 tacggcacaa ccctggcggt ggacaacata tccctgcgga tcccacgcgg cacctattgc
     1081 tgcctgctcg gtcccagcgg ctgcggcaag accacgacgc tgcggatgat cgccgggcat
     1141 gaggcggtga ccgagggcga tatcctgatc cacggcgaga atgtctccga cctgccgccg
     1201 atcaagcgcg gcaccgcgat gatgttccag agctatgcgc tgtttccgca catgtcgtgc
     1261 ctggacaatg tcgccttcag cctgaagatg cgcggcgtcg ccaagaagga gcgtcatgcc
     1321 caggcgcgcg aatacctgac actggtgcac atgacggatt acgccgaccg cctgccggcg
     1381 caactgtctg gtggtcagca gcagcgaatc gcgttggctc gggcgctgat caaccgcccg
     1441 cgggtgctgc tgctggacga accgctatcg gcgctcgacc cattcctgcg cgtcaaggtg
     1501 cgggaggaac tgaagcgcct gcagcgggaa ctcggcatta ccttcatcca cgtcacccac
//
LOCUS       DASWQN010001217         1854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2231217, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001217 DASWQN010000000
VERSION     DASWQN010001217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2231217"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VGC82_20670"
     CDS             <1..649
                     /locus_tag="VGC82_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY0425743.1"
                     /translation="DFDTALVTAAFRLAAENGWRQVSVVAAARDAGLSLAEARGRFPS
                     RGSVLLRFGQLADQAALTDAASEGPVRDRLFDLLMRRFDMLQRHRGGVTALLRVLPFD
                     PLTALMLAAATKRSMRWMLQGAGIQATGARGELQAQGLLAVWLWALRAWERDDSEDLS
                     GTMAAIDSALNRAEQVASWLHGQQPAPPPPPPVSEAAGELDPSVPPAGEPPVAGV"
     gene            646..1437
                     /locus_tag="VGC82_20675"
     CDS             646..1437
                     /locus_tag="VGC82_20675"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0425744.1"
                     /translation="MTDPLQARFDIAMRLAERAGEMAMSMRPPPGSARAVLKGPQDWV
                     TEADGAVERFLSEQLATEFPEDGFQGEEGGISRGGSLRWVVDPIDGTSNYARGANRFC
                     VSLACLEGDTPLIGVIAAPALRETACARLGAGAWLNGEAIRVAETRSAKQAIMEVGWS
                     RRRPDADYLNICARVLADGASLRHGGSGALGLADVAIGRQDGYAELHINLWDVAAGLV
                     ILREAGAKVSDFLAGNGATAGNAIVACAPGLAAAWSDLPGLPSPV"
     gene            1505..>1854
                     /locus_tag="VGC82_20680"
     CDS             1505..>1854
                     /locus_tag="VGC82_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEY0425745.1"
                     /translation="MASYDFATVNVFTDRRFGGNPLAVLPDANGLTDQQMQAIAAEFN
                     LSETTFVLPPTDPRHHARVRIFNRQHEMPFAGHPNVGTGYLLATMAGDPPEHYTFEEM
                     AGLVRVHILRDANRA"
BASE COUNT          281 a          570 c          678 g          325 t
ORIGIN      
        1 ggatttcgac accgccctgg tcactgcggc cttccgcctc gcggccgaga acggctggcg
       61 tcaggtcagc gtggtcgccg ctgcccggga cgccgggctg tcgctggccg aggcccgggg
      121 gcggtttccg tctcgcggtt cggtgctgct gcggtttggc cagcttgcgg atcaggccgc
      181 gctgacggat gccgcgtccg aagggccggt gcgggaccgg ttgttcgacc tgttgatgcg
      241 gcgcttcgac atgttgcaga ggcaccgggg cggggtgaca gccttgctgc gggtgctgcc
      301 cttcgacccg ctcacggcgc tgatgttggc agccgccaca aagcgcagca tgcgatggat
      361 gctccagggt gccggaattc aagcgaccgg cgcgcgcggg gagctgcagg cgcagggttt
      421 gctggcggtg tggctatggg ccctgcgagc ctgggaacgc gacgattcgg aggatttgtc
      481 cggcacgatg gcggcgatcg atagcgcgtt gaaccgggcg gaacaggttg cgtcgtggct
      541 gcatgggcag cagccggcgc ctcctccgcc gccgccggtc tcggaagccg ctggggaact
      601 cgatccgtcg gtaccgcccg ccggggaacc accggtggcc ggcgtgtgac cgatccgctt
      661 caggcgcgct tcgacattgc catgaggctg gccgaacggg ccggggaaat ggcgatgagt
      721 atgcgtccgc cgcccggatc ggcgcgggcg gtgctgaaag gaccgcaaga ctgggtgacc
      781 gaggcggatg gcgccgtcga acggttcctg tccgaacaac tcgccacgga attccccgag
      841 gatggattcc agggcgagga aggcgggatt tcgcgcggcg ggtcgctgcg ctgggtggtg
      901 gatccgatcg atggtacatc aaattacgcg cgtggggcca accggttctg cgtgtcgctg
      961 gcgtgcctgg agggagatac accgctgatc ggggtcatcg ccgcccccgc attgcgggaa
     1021 acggcttgtg cccggttggg cgccggggcg tggctcaacg gcgaggcaat ccgggtggct
     1081 gaaacccggt cggcaaagca ggcgatcatg gaggtcggct ggtcccggcg gcgtcccgat
     1141 gccgattacc tgaacatatg cgctcgtgtg ttagcggatg gcgccagctt gaggcatggc
     1201 ggttccggtg cgctgggact ggcggatgtc gcgatcgggc gacaggacgg ctacgccgag
     1261 ctgcatatca atctatggga tgtcgccgcg ggcctggtga tcttgcggga ggccggggcc
     1321 aaggtcagcg acttcctggc aggcaacggc gcgacggcgg gcaacgccat cgtggcctgt
     1381 gcgccgggcc tggccgcggc atggtcggac ttgccgggac taccgtcccc ggtctgatcg
     1441 catcgtctga cctcgcttag caacttcaga ggcagtatgt ctggatttcc cgcaggaggt
     1501 cgccatggcc agttatgatt tcgccaccgt caacgtcttc accgaccggc gctttggcgg
     1561 caacccgctg gctgtcctgc ccgatgccaa cggcctgacg gaccagcaga tgcaggcgat
     1621 cgcggcggaa ttcaacctgt cggaaacgac gttcgtgttg ccgccgaccg atcctcggca
     1681 ccacgcccga gtgcgtatct tcaaccgcca acacgagatg ccgttcgctg gacatccaaa
     1741 tgtcggcacc ggctaccttc tggcgacgat ggctggcgat ccccctgagc actacacatt
     1801 cgaggaaatg gcgggcctgg ttcgcgtgca catcctgcgc gatgcgaacc gggc
//
LOCUS       DASWQN010001218         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2362474, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001218 DASWQN010000000
VERSION     DASWQN010001218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2362474"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1184)
                     /locus_tag="VGC82_20685"
     CDS             complement(132..1184)
                     /locus_tag="VGC82_20685"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEY0425746.1"
                     /translation="MAPNTKRVFYVKYLPSAVYATLLAERPDVQLDRLENDSPGEQVA
                     PILTQGHVYQIGASRQIIAPHFQVNAALLARTPNLIAASSNGAGYDTIDVDACTEAGV
                     AVVNQSGGNKEAVAEHALAMMLTLSKHIAEANRQARTGQAIDRGTYIGRELNGRTVGV
                     LGIGNVGGRLAAMCRAAFNMRVLAYDPLLTVEQVKARGAEKVELEELLAQSDYVSVHC
                     PLTKQNRRMMGAAQFARMKPEAYFITTARGFIHDEEALADALRKKQIAGAGLDVWEDE
                     PPPHDHPLMAFDNVIVSPHIAGSTIEARENMGRIAAEQALAILDGKRPPRLINPEVWP
                     AYRERFERIMGFKPET"
     gene            complement(1268..>2609)
                     /locus_tag="VGC82_20690"
     CDS             complement(1268..>2609)
                     /locus_tag="VGC82_20690"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0425747.1"
                     /translation="VEDDGKRWKLRLREGLLWHDGEKVRAQDCVASIRRWAVRDGIGS
                     LLMKRTDELAAIDDRTIQFRLNRPFRMLPYALGKISTPMCAMMPERLAKTDPFKVVTE
                     MVGSGPFRFKADEWVSGARAVYTKFEGYVPRQEISKGWTAGGKIVHLQRVEWLTSPDD
                     GTSASAMHSGEMDWWELPTADLLPLLKRTGKIRVEIKDRNGTLGLLKMNNLQPPFNDP
                     AIRRALLGAVDQADFMTAIAGADPTMWRTGVGIFTPGTDMATDVGMNVLNSPRDLVAV
                     RRAIEAAGYKDEKTVLLAPSEPNYRKAMADVSAGMLKAVGFNLEVQSMDWGTAIVRRE
                     NKQPVDKGGWSALCSTANGLDMQTPLLHFLRTTGETAWFGWTSSPRIEELRAAWADAP
                     DLAAQKQIAADIQLQCWIDVPHLPLGLWYQPMAWQNTIDGIPDGFPLFWGVRRV"
BASE COUNT          461 a          878 c          736 g          534 t
ORIGIN      
        1 gaaaagcttc gggatatcgg ccgaggatgg gtcagcgccc ttcgttacgg ccggaccaga
       61 agggcccgcg cattccgacg tgccgctgga ggaagcttca cggcccgatc ggttggcgcg
      121 gctgtctccc gtcaggtctc cggcttgaat cccatgatcc gctcaaaccg ttcccgatag
      181 gccggccaga cctcgggatt gatcagccgc ggcggccttt ttccatccag tatcgccagc
      241 gcctgttccg ccgcgatccg tcccatgttc tcccgcgcct cgatcgtgga gccggcgatg
      301 tgcggactga cgatcacgtt gtcgaacgcc atcaaaggat ggtcgtgcgg cggcggctcg
      361 tcctcccaaa catcgagacc agcacctgcg atttgcttct tacgcaaagc gtccgccagg
      421 gcttcctcat cgtggatgaa gccgcgtgcg gtggtgatga agtaggcctc gggtttcatg
      481 cgggcgaact gggctgcgcc catcatccta cggttttgtt tcgtcagcgg gcaatgcacc
      541 gatacataat cggactgggc cagcaactct tccagctcga ccttctccgc gccgcgggct
      601 ttcacctgtt ccaccgttag aagaggatca taggccagca cccgcatgtt gaaggccgcg
      661 cgacacatgg cagccaaacg tccgccgacg ttgccgatgc caagcacgcc aaccgtgcgc
      721 ccgttcagct cgcggccgat ataggtgccg cgatcgatcg cctgccccgt ccgagcctgg
      781 cggttggctt cagcaatatg ttttgacaac gtcagcatca tcgccagggc gtgttcggca
      841 accgcttcct tgttgccacc cgactgattg accaccgcga ccccggcctc ggtgcaggca
      901 tcgacatcga tcgtgtcata gccggccccg ttcgacgacg cggcgatcag gttcggcgtg
      961 cgggcgagca atgctgcgtt gacctggaaa tgcggcgcga tgatctgccg tgaggcgcca
     1021 atttgatata cgtgcccctg ggtcaggatc ggcgcgacct gttcacccgg actatcgttt
     1081 tccagcctgt ccagttgcac atccggccgt tccgccagca gcgtcgcata caccgcggac
     1141 ggcaaatatt tcacgtagaa cacgcgcttg gtgttcggtg ccatgttggt tccctccttc
     1201 actcgtttcc tggcctacgc cgaccgcaca atcttccatc gacagtgagg cactcccttc
     1261 accaacatca aacccgccgc acgccccaga acaatgggaa cccatcggga atcccgtcga
     1321 tcgtgttctg ccacgccatc ggctgatacc agagcccaag cggcaaatgc ggcacgtcga
     1381 tccagcactg aagctggata tcggcggcga tctgcttctg cgccgcgaga tccggggcat
     1441 ccgcccaggc tgcgcgaagc tcctcgatgc ggggactgct ggtccagccg aaccatgcgg
     1501 tttcacccgt ggtgcgtaga aaatgcagca gcggcgtttg catatccaga ccgttggcgg
     1561 tggagcacaa tgcgctccag ccacctttgt ccaccggttg cttgttctcc cgccgcacga
     1621 tcgccgtgcc ccagtccatc gactgaacct ccaggttgaa gccaacggcc ttcagcatgc
     1681 cggcgctgac atcagccatg gccttgcggt aatttggttc gctcggcgcc agcaggacgg
     1741 ttttctcgtc cttgtagccg gctgcttcga tcgccctccg tacagccaca aggtcccgtg
     1801 ggctgttcag cacgttcatc ccgacgtccg tggccatgtc ggtgcccggc gtgaaaattc
     1861 ccacgccggt gcgccacatc gtcggatcgg cgccagcgat cgcagtcatg aaatccgcct
     1921 gatccacggc accaagcagt gctcgacgaa tcgccggatc gttgaacggc ggctgcaggt
     1981 tgttcatttt cagcaagccg agggtgccgt ttcggtcctt gatctccacc cgaatcttgc
     2041 ccgtccgttt caacagcggg agaaggtccg ctgtcggcag ttcccaccaa tccatctccc
     2101 cggagtgcat ggcactggcc gaagtgccgt catcgggact ggtaagccac tccacgcgct
     2161 gcagatgcac gatttttcca ccggcggtcc accctttgct gatttcctgt cttggaacat
     2221 agccctcgaa cttcgtgtaa acggctcggg cgcccgacac ccattcatcc gccttgaagc
     2281 gaaacggccc gcttccgacc atctcggtga ccaccttgaa cgggtctgtc ttagccaggc
     2341 gttccggcat catcgcgcac atcggtgtcg agatcttgcc cagcgcgtaa ggcagcatgc
     2401 ggaatggcct gttgagacgg aactgaattg tccggtcgtc gatcgcggcc agctcatcgg
     2461 tccgcttcat cagcaggctg ccgatgccgt cgcgcaccgc ccaacggcgg atcgaggcga
     2521 cacaatcctg cgcacgaacc ttttcgccgt cgtgccaaag aagtccttcg cgcagtctca
     2581 gcttccagcg ctttccgtca tcctccacc
//
LOCUS       DASWQN010001219         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_647701, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001219 DASWQN010000000
VERSION     DASWQN010001219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647701"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VGC82_20695"
     CDS             complement(<1..344)
                     /locus_tag="VGC82_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0425748.1"
                     /translation="MGKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKILAKTGGATY
                     TSYDQIDKAPEEKARGITISTAHVEYETKNRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMP"
     gene            complement(447..923)
                     /gene="rpsG"
                     /locus_tag="VGC82_20700"
     CDS             complement(447..923)
                     /gene="rpsG"
                     /locus_tag="VGC82_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY0425749.1"
                     /translation="MSRRHRADKREILPDAKFGDIVITRFMNALMYDGKKSAAEGIVY
                     NALDVMKRRGGAAADPVRMFHEALDNVKPAVEVRSRRVGGATYQVPVEVRTERRQALA
                     IRWIIDAARKRGEHTMQDRLSNELLDAVNNRGSAVKKREDTHRMAEANKAFSHYRW"
     gene            complement(936..1307)
                     /gene="rpsL"
                     /locus_tag="VGC82_20705"
     CDS             complement(936..1307)
                     /gene="rpsL"
                     /locus_tag="VGC82_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY0425750.1"
                     /translation="MPTINQLIAHGRERPKARNSVPALQGCPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGYEVVSYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHILRGVL
                     DTQGIAKRRQRRSLYGAKRPK"
     gene            1739..>3226
                     /gene="asnB"
                     /locus_tag="VGC82_20710"
     CDS             1739..>3226
                     /gene="asnB"
                     /locus_tag="VGC82_20710"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY0425751.1"
                     /translation="MCGIAGLILSSGAAPPDPAVLSKLIGALAHRGPDGSGHAVMGRV
                     ALLHNRLAIIDLVTGDQPFFSGPATLVANGEIYNYRELRESLPGMSFATSSDCEPPLH
                     LWLRDGVDYARQLRGMYAIAIHERVRRTVTLTRDPFGIKPLYIAMIDGGLAFASEPQA
                     LLEAGLVKRSVRPAAVEELLQLQFTTGADTIFTGIQRVLPGETITCADGHILERRRLN
                     PVPALPPEDIDEDTAMRRLDRALMESVDLHQRSDVPYGMFLSGGIDSATLITLMSRLN
                     DQPVLAFTAGFDAPGAADEREQAAAIARAVGARHESIEVSEKMVWQHLPEIVAAMDDP
                     TMDYAIIPSWFLARRAQQDVKVVLTGEGGDEIFGGYKRYRQATLPWWRGGQTMWAGGS
                     FDKVPVLRSRPTSWRDGMAAAEIRAAEGGRSPLAAAQALDMTDWLPHDLLLKLDRCMM
                     AHAVEGRTPFLDAGVMAAAFRLPDAMKLRRGMGKWILRKWLEDNQP"
BASE COUNT          534 a         1044 c         1035 g          613 t
ORIGIN      
        1 tggggcatcg gcccgtcggc cgcagacacg actaggatcg cgccgtccat ctgggcggca
       61 cccgtgatca tattcttcac gtagtcggcg tggccggggc aatcgacgtg cgcgtagtgg
      121 cgattcttgg tctcgtattc cacatgggcg gtggagatcg tgatgccgcg cgccttttct
      181 tccggcgcct tgtcgatctg gtcataggac gtgtacgtcg cgccaccggt cttggccagg
      241 attttagtga tcgcggccgt cagcgacgtc ttgccgtgat cgacgtggcc gatcgtgcca
      301 atgttgcagt gtggcttgtt ccgctcgaat ttcgccttac ccatcgtcgt gtctccgtag
      361 atccaccgga tcttttggtt gtgccgttac cttagtccga agcccggtcg gcgcccttcg
      421 gcatgccgcc taacaagacc aggcttttac caacgataat gactgaaggc cttgttggcc
      481 tctgccatcc ggtgggtgtc ttcgcgcttc ttcaccgcgg aaccgcggtt gttcaccgcg
      541 tccaacaatt cattggacag gcggtcctgc atcgtgtgtt ccccgcgctt gcgcgcggca
      601 tcgatgatcc accttatggc cagcgcctgc cgccgctccg tccggacttc gaccggcacc
      661 tgataggtgg cgccgccaac gcggcgcgag cggacttcga cggccggctt cacgttatcc
      721 agcgcctcgt ggaacatccg caccggatcg gcggccgcgc cgccccgacg cttcatcacg
      781 tcgagggcat tgtacacgat gccttcggcg gccgacttct tgccgtcgta catcagcgca
      841 ttcatgaagc gggtgatgac gatgtcgccg aacttcgcgt ccggcagaat ttcgcgctta
      901 tctgcgcggt gacgacgaga catatgaccg cccttctact tcggccgctt cgcgccatac
      961 agcgaacggc gctggcggcg tttggcgatg ccctgggtat ccaggacgcc acgcaggata
     1021 tggtagcgaa cgcccggaag atccttcacg cgtccaccgc ggatcaggac gaccgaatgc
     1081 tcctgcagat tgtgaccttc gccgggaatg tagctgacga cttcatagcc gttggtgagg
     1141 cgaaccttcg ccaccttgcg cagcgccgag ttcggcttct tcggcgtcgt ggtatagacg
     1201 cgcgtgcaaa cgcctcgctt ctgcgggcag ccttgcagcg ccggaaccga gttcctggct
     1261 ttcggccgct cgcgcccatg ggcgataagc tggttgatgg tcggcatgga cctctttctc
     1321 tttccgttcg cgcttcgccg cggtctggtc gggtcgccgc ttaataggca aagcccgccg
     1381 gcatggggaa gatcccatcc tgcggggtga cggtgggccc acggcctaag cccgattgaa
     1441 ttcgggcggc gccgcatgcg tcgtatgcct gcaaaccagc gaccatccca aatgacccgg
     1501 gtcggccgag ggcgcgatct ctagaagccc gttccaaccg agtcaagcgt tgaagccagc
     1561 ctatattgcc gattcgagca ttctggcagc cgcttaatcc gaatgaaact cgtcccacgc
     1621 atggttcggg acgacaagac gaggcaagtg cattaagcac ctcgcggtaa ccgggacctt
     1681 cgaaggcgga ggaaccggtc cggcaccggc gatacgacat tcggagacac acccatatat
     1741 gtgcggcatc gccgggctga tcctttcctc tggcgccgcg ccacccgatc cggcggtgtt
     1801 gtccaagctg atcggagcgt tggcccatcg cggcccggac ggcagcggcc acgccgtgat
     1861 gggccgggtg gccctgctgc ataaccggct ggcgatcatc gacctggtca ccggcgacca
     1921 gccattcttt tccggacccg ccacgctggt ggcgaacggc gagatctaca attaccgcga
     1981 gctgcgcgaa tcgctgccgg gaatgagctt cgcgaccagc agtgactgcg agccgccgct
     2041 gcacctgtgg ctgcgcgacg gcgtggacta cgccaggcag cttcgcggca tgtatgcgat
     2101 cgccattcac gaacgcgtgc gccgtaccgt taccctgacg cgcgatccat ttggcatcaa
     2161 gccgctttat atcgccatga tcgacggcgg cctggctttc gcctcggaac cacaggccct
     2221 gctggaggcc ggcctggtca agcggtcggt gcgtccagcg gcggtcgagg agctgctgca
     2281 gctgcagttc accacgggcg ccgatacgat ctttacgggc atccagcgcg tcctgcccgg
     2341 cgaaaccatc acctgcgccg atggacatat cctggaacgc cggcggctca atccggtacc
     2401 ggccctgccg ccggaagata tcgacgagga caccgcgatg cggcggttgg atcgggcgtt
     2461 gatggaaagc gtcgatctgc atcagcgcag cgatgttccg tatgggatgt tcctgtccgg
     2521 cggcatcgac agcgccacct tgatcacctt gatgtcgcgg ctgaacgatc agcccgtgct
     2581 ggccttcacg gccggcttcg acgcgcccgg cgccgccgat gagcgcgagc aggcagccgc
     2641 catcgcccgc gcggtgggcg ctcggcacga gtcgatcgag gtatcggaaa aaatggtctg
     2701 gcagcatctg ccggaaatcg tcgcggcgat ggacgatccc accatggact atgccatcat
     2761 cccgtcctgg ttcctggcgc ggcgcgccca gcaggacgtg aaagttgtgc tgaccggaga
     2821 gggcggcgac gagattttcg gcggctacaa gcgctaccgt caggcgacgc tgccgtggtg
     2881 gcgcggcgga cagaccatgt gggccggcgg cagtttcgac aaggtgcccg tgctgcgcag
     2941 cagacccacc tcctggcggg acggcatggc cgcggcggaa attcgggcgg ccgagggcgg
     3001 acgctccccg ctcgcggcgg cgcaggcgct ggacatgacg gactggctgc cacacgacct
     3061 gctgctgaaa ctggaccgct gcatgatggc ccacgcggtc gaaggccgca cgccgttcct
     3121 cgacgccggc gtgatggccg cggcgttccg cctgcccgat gcgatgaagc tgcggcgcgg
     3181 catgggcaaa tggattctcc gtaagtggct ggaggacaat cagccg
//
LOCUS       DASWQN010001220         3999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2099908, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001220 DASWQN010000000
VERSION     DASWQN010001220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099908"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VGC82_20715"
     CDS             complement(<1..784)
                     /locus_tag="VGC82_20715"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0425752.1"
                     /translation="MPRIIRFRDLEPSWTTKQAKEPGFMRWLVTWVGGPAGHINTNPG
                     IAVESAQCAVGMMYLPRGQRQAGKHTHGVTEVYVVLQGELDGFDASGHPHRAGPMDCT
                     YIPAGCPHGVRNCGLQDVVLIWVHDGIERNGTAVYYPDDHEFTNAPPIELVRFADLEP
                     DWSAPGARTPGTMRWSVNWVDAQEDVRKHNPATAIYNAKIAIGMTVLEPGHCNPAEAL
                     PVSRLYLVVEGEAIIDTGGRTTTLGHLDGLHLPAGEAATLRNN"
     gene            complement(898..2556)
                     /locus_tag="VGC82_20720"
     CDS             complement(898..2556)
                     /locus_tag="VGC82_20720"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0425753.1"
                     /translation="MAIRKGVAMANAVTTNKSGHAGEFDALVIGAGFSGLYQLFCLRD
                     RLGLSVQVLEAGEGVGGTWYWNRYPGARCDSESHAYCYSFSKELTQELEWSERYPEQP
                     EIMRYLNYVADRFDLKRDIRFNTRVVAASYDEAANRWTVRTEAGEQFTTKFLITAVGC
                     LSTANVPKIPGLESFEGSWYHTGQWPHEGVDFTGKRVGMIGTGSTGIQAAPVIAETAA
                     HLTVFQRTANYSVPARNAPLTPEFKRYIKENATQIRAVMHATRNGMPFLISERQALAT
                     PPEERDALYEAAWKKGGLEFRATFEDIMVKKAADDTAADFIRRKIRAIVKDPATAAKL
                     SDIDHPYATKRPPIDTNYFETFNRDNVTLVDVKATPIERITPTGVRTSDADHPLDIIV
                     FATGFDAMTGPMLRMNITGRDGISLAEAWEAGPRTYLGLQIVGFPNLFTMTGPGSSSV
                     LCNMPVPIEQYAEWIADCIAHMRAHDLGRIEATQEAVTNWVEQVNAAANTTLLPLAKS
                     SWYLGANVPGKPRVFMPYAGGMARYRTICADVAANNYEGFVFSE"
     gene            2652..2945
                     /locus_tag="VGC82_20725"
     CDS             2652..2945
                     /locus_tag="VGC82_20725"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0425754.1"
                     /translation="MPTERSPNRFDFAPGESRAVLASFDGRRITADAGALLLGAADRV
                     TGLTRCFASCFTDHRNPAFVEHSVQQELPRPQCQPWVIGSLKADRLLTQRSLG"
     gene            3292..>3999
                     /locus_tag="VGC82_20730"
     CDS             3292..>3999
                     /locus_tag="VGC82_20730"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0425755.1"
                     /translation="MPDTHVLAERTAQDQVATYDAIVIGAGISGMFMLYRLRELGMTA
                     RVLEAGTNVGGTWYWNRYPGARFDSESWTYGYSFSKELMQEWDWKEHFSPQPDTLEYL
                     NHVADKFDLRRDMQFRSTVTAAHWDDATDEWTVTLASGQQAKSRFLCTAIGILSAHTL
                     PAIPGRESFHGPAYHPARWPHTPVDFTGKRVGIIGTGATAIQAIPEIAKQAKQLTIFQ
                     RRPNWAAPLHNAKISKEE"
BASE COUNT          745 a         1280 c         1194 g          780 t
ORIGIN      
        1 cgttattacg gagcgtagcg gcctcgcccg ccgggaggtg cagtccatcg agatgaccaa
       61 gtgttgtggt gcgtccgccg gtatcaatga tggcctcgcc ttcgacgacg agatagaggc
      121 gggagactgg cagcgcctca gcaggattgc agtggccagg ctccagcacc gtcatgccga
      181 tggcgatttt ggcgttgtaa atagcggttg ctggattgtg cttgcgcacg tcttcctggg
      241 catcgaccca gttgaccgac caacgcatcg tgccgggtgt tcgagcccct ggcgcggacc
      301 aatcgggttc caaatcggca aaccgcacca gttcgatagg cggtgcattg gtgaattcat
      361 ggtcgtccgg atagtagacg gctgtgccgt tccgctcgat cccatcatgc acccatatca
      421 ggacgacgtc ctgcaggcca caattgcgca ccccgtgggg acaaccggcc gggatgtagg
      481 tgcaatccat cggccccgcg cggtgcggat gaccggaggc atcaaagcca tccagctctc
      541 cctgcagcac cacgtagacc tccgtcacgc catgcgtgtg cttgcccgcc tggcgctggc
      601 cacgcggcaa atacatcatg cctaccgcgc actgtgcgct ctctacagcg attccaggat
      661 tggtgttgat gtgtccagcg gggcctccca cccaggtgac aagccagcgc ataaagcccg
      721 gctctttcgc ctgcttcgta gtccaggagg gttccaggtc gcggaaccgg atgatgcgcg
      781 gcattatttg ctcccccttg aggagggagc ctagccgccg attccaataa gtgtcgaact
      841 tagtgactgc tgccgtgcga ctctgttcga atgctgcccg accttcagcc gtccgcttca
      901 ctcactgaag acaaagccct cgtagttatt ggccgcgaca tccgcacaga tagttctgta
      961 acgcgccatc ccgccagcat aaggcatgaa cacgcgaggc ttcccaggga cgttcgcgcc
     1021 gaggtaccag gaactcttcg ccagcggcaa cagggtggtg ttggcggccg cgttgacctg
     1081 ttccacccag ttggtcaccg cctcctgcgt ggcctcgatg cgtcccaggt catgagcgcg
     1141 catatgggcg atgcaatcgg caatccattc cgcgtactgt tcgatcggca ccggcatgtt
     1201 gcaaagcacg gacgagctgc cggggcccgt catggtgaac agatttggga accccacgat
     1261 ctgcagcccg aggtaggtgc gtgggccggc ttcccatgcc tctgccaatg agatcccgtc
     1321 gcggccggtg atgttcatgc gcagcatcgg gccggtcatg gcatcgaagc ctgtcgcaaa
     1381 gacgatgatg tccaatggat gatctgcgtc acttgtgcgg acgccggtcg gcgtgatccg
     1441 ctcgatcggg gtggctttca catcgaccag cgtgacgttg tcgcggttga aggtctcgaa
     1501 atagttggtg tcgatgggcg gccgcttcgt cgcataggga tggtcgatgt cagacagttt
     1561 cgccgccgtc gcggggtcct ttacgatcgc gcggatcttg cggcggatga aatccgcagc
     1621 cgtgtcgtcc gctgcctttt tcaccattat atcctcgaag gtggcgcgga actccagccc
     1681 tcccttcttc caggccgctt cgtataacgc gtcacgttcc tccggcggcg tggccagcgc
     1741 ctgacgctct gaaatcagga acggcatacc atttcgggtg gcgtgcatga cggcccgaat
     1801 ttgggtggcg ttttccttga tgtagcgctt gaactccggg gttagcgggg cattgcgggc
     1861 tggcacgctg tagttggctg tgcgctggaa aaccgtaaga tgggcggccg tctcggcgat
     1921 caccggcgcg gcctggatgc cggtggagcc ggtgccgatc atgccgaccc gtttaccggt
     1981 gaaatccacc ccttcatgcg gccattgccc agtgtgatac cagcttccct caaagctttc
     2041 gaggccaggg atcttcggga cattcgcggt ggacaggcaa ccgacggcgg tgatcaggaa
     2101 cttcgtagta aactgctcgc cggcctcggt gcgcacggtc catcggttcg ccgcttcgtc
     2161 gtaactggcc gcgacgacgc gggtattgaa gcggatgtcc cgcttcaggt cgaaccgatc
     2221 tgccacataa ttgaggtaac gcatgatctc cggctgctcc ggataccgtt cggaccattc
     2281 caactcttgc gtcaattctt tggagaacga atagcagtag gcatgacttt ccgaatcgca
     2341 tcgcgcgccg ggatagcggt tccagtacca ggtgccgcca acgccctccc ctgcctccag
     2401 cacctgcacc gaaagaccga gccggtcgcg caggcagaac aactggtaga ggccggagaa
     2461 ccccgcccca atgaccaggg catcgaattc gccggcgtgg ccggatttgt ttgtcgtcac
     2521 cgcgtttgcc atcgccacgc cttttctgat tgccaacccg cacttctcac acaattcagg
     2581 cgcatcggga ccttagatcg gcaaaagctc gtctgcggca aagccttgcc gctgaacaag
     2641 ggaggccccc gatgccgaca gagcgtagcc cgaaccgctt tgattttgca cctggtgaaa
     2701 gccgtgcggt ccttgcgtcc tttgacggcc gccgcatcac cgccgacgcc ggcgccctgc
     2761 tgctgggcgc ggccgatcgc gtcaccggcc tgacacgctg cttcgcctcc tgcttcaccg
     2821 accatcgcaa tcccgccttt gtcgagcata gcgtgcagca ggaactgcct cggccgcaat
     2881 gccagccttg ggtcatcgga tcgctaaaag cggaccgact ccttactcag cggtcgctag
     2941 gttaggcaat acgtgacgca ggaaaaccca gcttggcact atgacgtttt cgctggttag
     3001 ccggccaagc taccgactgc tgagtaatgc gccttggcag ctcattcgtg gggcacgcgg
     3061 cgaccccgga ggtagccgtt cctggacatc cgcatccgtc gacaagcata cgtccgacgg
     3121 caacccaata aggtcgtatc cgccccattc ccacgccggt ctcccgatcc ttgcggcctg
     3181 tccggttaaa gggatagcgt ggcaacgacg gaacggttcg ctgcaaatac cgcgtgatcg
     3241 acgggaagcg ccgcggattt tggccgtgcg cgccaaaggg ggagacacac catgccggac
     3301 acccacgtcc tggcggaacg gacggctcag gatcaggtcg cgacctacga cgcaattgtc
     3361 atcggcgccg gcatttccgg catgttcatg ctctaccgcc tgcgtgaact tggcatgacg
     3421 gcgcgggtcc tcgaagccgg caccaacgtc ggcggcacct ggtactggaa ccgctatccc
     3481 ggcgcccgct tcgattccga aagctggacc tatggctatt cgttttcaaa ggagctgatg
     3541 caggagtggg actggaagga acatttttcc cctcagcccg acacgctgga gtacctgaac
     3601 catgtggccg acaagttcga cctgcgccgg gacatgcagt tccgcagcac ggtgacggcc
     3661 gcgcattggg atgacgccac ggacgaatgg accgtgaccc tcgcaagcgg ccagcaggcg
     3721 aagtcgcgct ttctgtgcac cgccatcggc atcctgtccg cccacactct gcccgccatt
     3781 cctggccggg aaagcttcca cggccccgcc taccaccccg cgcgctggcc gcacacaccg
     3841 gtggacttca ccggtaagcg cgtcggcatc atcggcaccg gggccaccgc cattcaggcg
     3901 atccccgaga tcgccaagca agcaaagcag ctcaccatct tccagcgacg cccaaactgg
     3961 gccgcgccgt tgcacaatgc caaaatcagc aaggaagag
//
LOCUS       DASWQN010001221         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_120214, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001221 DASWQN010000000
VERSION     DASWQN010001221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120214"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1076)
                     /locus_tag="VGC82_20735"
     CDS             complement(99..1076)
                     /locus_tag="VGC82_20735"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY0425756.1"
                     /translation="MKVGIFGAGAIGGFLGVRLARAGVDVTFVARGPHLAAMQANGVT
                     LRSAGDSVTVRPRCVASAEEAGVQDYVVVTLKAHGLPPAAPAVAAMMGPAGAVVTGVN
                     GVPYWYFYGLDGPYRDRVVESVDPGGRLWTELPPSRAIGCVVYPAAEVVAPGVIEHTY
                     GDRFTLGEPDGSRSPRVECLSKAMIAAGLKAPVRPRIRDELWVKLWGNLAFNPISALT
                     GATLDRIIADHDLRTLCRGMMLEAKAAAEALGVRFSIDVDKRIAGGADVGVHKTSMLQ
                     DLERGRPMEIDALLGAVVELGALVGEAMPLCRTVLALVRERARIAGCYT"
     gene            complement(1073..>2140)
                     /locus_tag="VGC82_20740"
     CDS             complement(1073..>2140)
                     /locus_tag="VGC82_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0425757.1"
                     /translation="NAEAEDVALILHTSGTTSRPKIVPLRHVNLTASAYNIGASLALC
                     PDDVCMNIMPLFHIHGLVAATLASLAAGGAVSCTAGFNGFRFYSWFEQVRPTWYTAVP
                     TMHQTILELAPRHKQAIADGRLRFIRSSSSSLPAKVMTAMEASFGVPLIESYGMTEAA
                     HQMASNPLPPGRRFPGCVGIAAGPEIAIMDEFGVLLPSGERGEVVIRGRNVTAGYENN
                     PTANASAFTNGWFRTGDQGVLDAEGYLRLTGRLKEQINRGGEKFSPLEVDEVLMDHPA
                     VAQCLTFGVPHAKLGEEVGVAVVLRPGQAAKDTELRAFCAERLAHFKVPRRIVILDEI
                     PKGATGKLQRIGLAEKLGLMA"
BASE COUNT          395 a          753 c          623 g          369 t
ORIGIN      
        1 ttcgggcgga taagccttgg atgccatgca ttcgcccaac atgacaagga gcggaaatgc
       61 ccggacaagg cgcagcgtag gcggcgaagc cagttgcatc aagtataaca accggcgatc
      121 cgggctcgtt cgcgcaccag cgccagcacg gtccggcaca gcggcatcgc ctcgcccacc
      181 agggctccta gttccacgac cgcacctagc agcgcgtcga tctccatagg gcgcccgcgc
      241 tccagatcct gcagcatcga tgtcttgtgc acgcccacgt ccgctccgcc ggcaatccgc
      301 ttatcgacat cgatcgaaaa tcggactcca agcgcctcgg ccgccgcttt cgcttccaac
      361 atcatgccgc ggcacagcgt ccggaggtca tggtccgcga taattcggtc cagcgttgca
      421 ccagtcagcg ccgaaattgg attgaacgcc agattgcccc acagcttcac ccaaagctca
      481 tccctgatcc gcggccgcac cggagccttc aatccggccg cgatcatcgc cttcgacagg
      541 cattcgacac gcggactgcg ggatccatcc ggctctccca gggtgaaccg gtcgccgtag
      601 gtatgctcga ttaccccggg tgccaccacc tcggcggccg gataaaccac gcagccgatc
      661 gcccgactcg gcggcagttc ggtccataac cggccgccag gatccacgct ttcgaccacc
      721 cggtcgcgat acggcccatc caatccgtag aaataccagt acggcacgcc gttgacgccg
      781 gtcaccacgg cgccggcggg tcccatcatc gccgcgacgg cgggtgcggc cggcggcagc
      841 ccgtgcgcct tcagcgtcac gacgacgtag tcctgcacac cggcttcctc agccgaggcg
      901 acacagcggg gccgtactgt cacgctgtcg cctgccgacc gcagcgtcac cccgttcgcc
      961 tgcatcgccg ccagatgcgg tccacgcgcg acgaacgtca catccacgcc agcccgggcc
     1021 agtcggacac cgagaaatcc accaatggcc ccagcgccaa aaattccgac tttcatgcca
     1081 tcaacccgag tttctcggcc aggccgatgc gttgcagctt gccggtcgct cccttcggga
     1141 tttcgtccag gatgacgatc cgccgcggca ccttgaaatg cgccagccgc tctgcgcaaa
     1201 aggcccgcaa ttccgtgtcc ttcgccgctt gcccgggccg tagcacgacc gccacgccga
     1261 cctcctcgcc aagcttggca tgcggtacac cgaacgtcag gcattgcgcc acggctggat
     1321 ggtccatcag cacctcgtca acctccaacg gactgaattt ttcaccgccc cggttgatct
     1381 gttctttcag gcgcccggtc agccgcaagt atccctcggc gtcaagaacg ccttgatcgc
     1441 cggtgcgaaa ccagccgttg gtgaatgcgc tggcgttggc ggttggattg ttctcatatc
     1501 cggcggtcac gtttcggccg cggatcacga cctcgccgcg ttcgccggat ggcagcagaa
     1561 cgccgaactc gtccatgatc gcgatctccg gtcccgccgc gatcccgacg cagccaggga
     1621 accgcctgcc gggcggcagc ggattggagg ccatctgatg cgctgcctcg gtcatgccat
     1681 acgactcgat cagcggcacg ccgaacgaag cttccatcgc cgtcatcacc tttgccggca
     1741 gcgaggacga tgatgagcgg atgaaccgca gccgcccgtc ggcgatcgct tgtttgtggc
     1801 gcggcgccag ttccaggatc gtttggtgca tcgttggtac cgcggtgtac caggtcggac
     1861 gaacttgttc gaaccaggaa tagaaccgga agccattgaa cccggcggtg cacgataccg
     1921 cgccgcccgc ggccaagctt gccagcgtgg ccgcgaccaa cccgtggatg tgaaatagcg
     1981 gcatgatgtt catgcagaca tcgtccggac acagtgccag actcgcaccg atgttataag
     2041 cggaggcggt caggttgacg tgccgcagtg gcacgatctt cggtcgagac gtggtgccgg
     2101 aggtgtgcag gatcagtgcc acatcctcgg cttccgcgtt
//
LOCUS       DASWQN010001222         4697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1440354, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001222 DASWQN010000000
VERSION     DASWQN010001222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440354"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VGC82_20745"
     CDS             <1..524
                     /locus_tag="VGC82_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY0425758.1"
                     /translation="AVRSMPFAVDRRFRVLQFVSSSMLSLAHGGNDAQKTMGIITVLL
                     YSQGLLTGGFHVPLWVVLSCQGAMALGTLCGGWRIVRTVGSRITHLTPMQGFCAETGA
                     ALSLFGATWLGIPVSTTHTVTGSIVGVGSARRASAVRWGVTHGIAVAWVVTLPASALV
                     AAACYRLAVLFVG"
     gene            complement(849..1922)
                     /locus_tag="VGC82_20750"
     CDS             complement(849..1922)
                     /locus_tag="VGC82_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0425759.1"
                     /translation="MAQIGDSKGEFRGITYRNDAELKAWLAKRPTEAALEPDLPIIDP
                     HHHFWDSPGRGTYLLPDLLADLGGGHNIVSTVFLECRAMYRKDGPSEMAPVGEVEFVN
                     GIAAMSASGNYGPCRVAEAIVGGGDLTVGARLRELLEAEMAVSGGRLRGIRHGVAWDG
                     SEAVGRFASRVVPPRLVRDAKFREGFGELAKLGLSFESWQYHPQLSDAIDLARAFPDT
                     TIILNHVGGVLGVGPYSGRRQEILAGWRKDINEFAKCPNVYVKLGGIGMTSFGFDFHE
                     RDVPPSSEDLAAAWRQYIEPCIEAFGVDRCMFESNFPPDKQSCGYTELWNAFKRITAN
                     ASAAEKTALYSGTAARVYRITAP"
     gene            2455..2955
                     /locus_tag="VGC82_20755"
     CDS             2455..2955
                     /locus_tag="VGC82_20755"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY0425760.1"
                     /translation="MARQPAGAQADAARKRPQEGDLLVRADSTDLTPLTPLSLALGEG
                     PLLALPYDPEAKSVRGGSRLNRILLLRLDPASLDASTAQRAADGVVAYSAICPHTGCE
                     VTNWHPDTQMLECPCHNSQYDPKAGARLVAGPSPRGLAALPLRIADGTLAVAHEFIGK
                     LGMQQM"
     gene            3007..>4697
                     /locus_tag="VGC82_20760"
     CDS             3007..>4697
                     /locus_tag="VGC82_20760"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEY0425761.1"
                     /translation="MRNRKTTRRITASAALTLGLLAIPAFARAQAANPPPAPPAAPNP
                     MPANGDPVASQAPMAAVLANYQPVTAERLTNPSDGDWLMFRRTYNGWGYSPLTQITAA
                     NVGSLKLVWTLTTGQLEGHQAPPIVNNGVMFVATPGNQVMAVNATTGDLLWRYKRPIP
                     EDMLQLHPTSRGVALWGDKVLFAATDATIVALDAKTGKEAWTTTVEDYAKGYYMSLAP
                     LVVDGKVIVGTSGGELGVRGFISAYDVATGKELWKTFTVPAPGEPGSETWPKGDQWKT
                     GGGSIWVTGVYDPETKLTFWGTGNGGSWMGDQRPGDNLYTSSVVALDVATGKIKGHHQ
                     YHPNDSWDWDEVSPPIIVDYQHNGKTIKGLVDVARDGYLWMLERTADKINFVAGQPFV
                     YHNTFTDLDPKTGRPNVAEEHKPGTGKLADFCPSLWGGKDWPPVAFSPKTKLLYIPAN
                     ENLCSTITGRPVKYIPGQIFTGAVSALYLHKGADHIGELQAWNLDTGKKVWTHDFGMS
                     PNWGPVLATAGNVLFAGGTNDRKFRAFDATDGKVLFEVPATSGVNGVPMSFEAGG"
BASE COUNT          879 a         1522 c         1443 g          853 t
ORIGIN      
        1 tggccgttcg gtccatgccg ttcgcagtgg accggcgatt ccgcgtcctg cagttcgtat
       61 cgtcgtccat gcttagcctg gcgcatggcg gcaacgatgc acaaaaaacc atggggatca
      121 tcacggtgct gctctattcc caagggctgc tgaccggtgg cttccacgtc ccgctgtggg
      181 tggtactgag ctgccagggc gctatggcac tgggcacgct gtgcggcggg tggcggatcg
      241 tgcgcacggt tggctcccgc atcacccacc ttaccccgat gcagggtttc tgcgccgaaa
      301 ccggcgccgc gctgtcgctg ttcggcgcca cctggctcgg gatcccggtg tccacgacgc
      361 atacggttac cgggtccatc gtcggagtcg gctccgcacg gcgcgcatcc gcggtccgtt
      421 ggggtgtgac acacggcatc gccgtggcct gggtcgttac gctacccgcg tcagccttgg
      481 ttgccgcggc atgttatcgc ctggccgttt tattcgtggg atgaacgcac gagcgctgcg
      541 cttactcatg cgtcctcgtg cacgatggaa atcgtgccat cgtgctcggc caccgcatag
      601 cgaacctggt caagtcgcat aaggccacgc tacgcggcaa tcgcaggcag ctgccggcac
      661 cttgaagtta accggacctt gaagttaacc ggcgtcttga cggttggcgc cgaggcacgc
      721 cgctgcttat tccaagcacc caagctgcgc cggccagtcg aatcacgtcc ttcgcaacaa
      781 cattttcccc ccggttcgtt gatgtcgctg gtcccggtca ggatcgccaa aaggggccat
      841 gacaaggttc agggcgcggt gatccgatag acccgcgccg ccgtcccgct gtagagggcg
      901 gtcttttcgg ccgccgacgc gttggctgtg atgcgcttga acgcgttcca cagttcggta
      961 tagccgcagg attgcttgtc tggtggaaag ttgctctcga acatacagcg gtcgacgccg
     1021 aatgcttcga tgcagggctc gatatactgc cgccacgcgg cggcgagatc ctcggatgac
     1081 ggcggaacgt cacgctcgtg aaagtcgaag ccgaacgacg tcatgccgat gccgccaagc
     1141 ttgacataaa cgttcgggca tttcgcgaac tcgttgatgt cctttcgcca tccggcgagg
     1201 atttcttgcc gcctgcccga ataggggcca acaccgagca ccccgccgac atgatttaga
     1261 atgatggtcg tatccggaaa ggcacgagcc agatcgatcg catcggatag ttgcggatga
     1321 tactgccacg actcgaagct gaggccgagc ttggccaact caccgaaacc ttcccggaat
     1381 tttgcgtcac gcaccagacg cggcgggacc actcgggagg cgaaccggcc caccgcctca
     1441 ctgccatccc atgccacgcc gtggcggatc ccgcggaggc gcccgccact gacggccatc
     1501 tcggcttcca acaactcccg caggcgggcg ccgacggtca gatcgccgcc gccgacgatc
     1561 gcctcggcca cacggcaggg gccataattg ccggaagcgc tcatggccgc gattccgttg
     1621 acgaactcga cctcgcccac cggcgccatc tcgctcggcc cgtccttgcg atacatcgcg
     1681 cggcattcga ggaagacggt tgagacgatg ttgtgcccgc cgccgagatc cgccagcagg
     1741 tcggggagca ggtatgtgcc gcgccccggc gaatcccaga aatgatggtg cggatcgatg
     1801 atcggcagat ccggttccag cgccgcttcg gtaggccgct tcgccagcca tgccttcagt
     1861 tcagcgtcat tccgatacgt gatgccgcga aattcgcctt tcgagtcccc gatctgagcc
     1921 atcttcgttc cctccatttg ccagcgcatt cttgtcgatt gccgctcgtg cggtgcccct
     1981 gggcaattcg ccactgatcg cgatgttgga accgtgactt caatcccgca aggcttcgtg
     2041 gcgttcgcct gcggtgcggc aaacctgtcg gcgttcatcg gcggcttatt cttgttggca
     2101 tccactgttg ccagaccatc atggcatgag cggaaacatg ccgattggat accggcgtat
     2161 cagcggccga atgcgggagt ccctaccgga cggtcgcggg tcgcatgaca atgtcgcggg
     2221 tcgcgtgaca atactgaacc ttgctgatag cattcccgaa taagaacgtg aagcgaacag
     2281 cggaggtggg atcgtgatat tcgcccatcc ggtgcggacc ggggaatttc tgaatcggcg
     2341 ggcaaccgcc cgatgaccgc cccggacgat aatgattgcc gcacctgcct caccgccgtt
     2401 tcggcaacca ggcgccatgc cctccttacc gcaagcgttg gaatcaccgt tgcgatggca
     2461 aggcagccgg ctggcgcgca agccgacgct gcccgcaaac ggccccagga aggcgacctg
     2521 ctggtcaggg ccgattcgac cgatctcacg ccgttgacgc ccttgtcctt ggcgctgggg
     2581 gaaggaccat tgttggcctt gccctacgat ccggaagcaa aatcggttcg cggaggctcg
     2641 cgtctcaacc gcatcctgtt gctgcgtctg gatcccgcct cgctcgacgc atcgaccgct
     2701 cagcgcgcgg cggatggcgt tgtcgcatac tccgcgatct gtccgcatac cggctgtgag
     2761 gtgacgaact ggcatcccga cacgcagatg ctggagtgcc cgtgccacaa ctcgcagtac
     2821 gacccaaaag caggtgccag gctggtcgcg gggccgtcgc cgcgcgggtt ggcggcgctg
     2881 ccgctgcgga tcgccgatgg caccctggcg gtggctcacg aattcatcgg caagctgggg
     2941 atgcagcaaa tgtagccgcc cgtacacagt cctagtcgta tgacggcaca aaaacaggag
     3001 ggaaacgtgc gcaacagaaa gacgacccga aggataacgg ccagcgccgc actgacgctg
     3061 ggcctgctcg cgatccccgc gttcgcccgg gcgcaagcgg cgaacccacc acccgcgccg
     3121 cccgcggccc cgaaccctat gcccgccaac ggcgatcctg ttgcgtccca ggcaccgatg
     3181 gccgcggtcc tcgccaacta tcagccggtg acggcggagc ggctgaccaa cccctccgat
     3241 ggggattggc tgatgttccg ccgcacctac aacggctggg gctacagtcc gctgacgcag
     3301 atcaccgccg ccaacgtcgg cagcctgaaa ttggtctgga cgctcacgac cgggcagttg
     3361 gagggtcacc aggccccgcc gatcgtcaac aacggtgtca tgttcgtcgc cacgcccggc
     3421 aaccaggtca tggcggtcaa tgccacgact ggcgacctgt tgtggcgcta caagcggccg
     3481 attcccgagg acatgctgca attgcatccg accagccgtg gcgtcgcgct ctggggcgac
     3541 aaggtgctgt ttgccgccac cgacgccacg atcgtcgcgc tcgatgcgaa gaccggcaag
     3601 gaggcctgga ccaccaccgt cgaagactat gccaaaggct actacatgtc gctcgccccc
     3661 cttgtggtgg acggcaaggt tatcgtcggc acctctggcg gcgaattggg cgtgcgcggc
     3721 ttcatctcgg cctatgacgt ggcgaccggc aaggaactat ggaagacctt caccgtcccg
     3781 gcgccgggcg agcctggcag cgagacctgg ccgaagggtg accagtggaa gaccggcggc
     3841 ggctcgatct gggtgaccgg cgtgtacgac ccggaaacca agctgacctt ctggggcacc
     3901 ggcaatggcg gctcatggat gggggatcaa cggccaggcg acaaccttta tacttcgtcg
     3961 gtcgtggcgc tggatgtcgc caccggcaag atcaagggac accaccagta tcaccccaac
     4021 gattcgtggg attgggacga ggtctcgccg ccgatcatcg ttgactacca gcacaacggc
     4081 aagacgatca aaggcctggt cgacgtcgct cgcgacggtt acctgtggat gcttgagcgt
     4141 accgcagaca agatcaactt cgtcgcgggc caacctttcg tttaccacaa cacgttcact
     4201 gacctcgatc cgaagacggg acgcccgaac gtcgccgaag agcacaagcc cgggaccggc
     4261 aagttggctg atttctgccc cagtttgtgg ggcggcaagg attggccgcc ggtggcattc
     4321 agccccaaga ccaagctgct ttatattccg gcaaacgaaa atctttgctc taccatcacc
     4381 gggcggccag taaaatatat ccccggccaa atattcaccg gcgcggtcag cgcgctatac
     4441 ttgcacaaag gcgcggatca tatcggcgaa ttgcaggcct ggaacctcga taccggcaag
     4501 aaggtctgga cgcatgactt cggcatgagc ccgaactggg gaccggtcct cgccaccgcc
     4561 ggaaacgtgc tgttcgcagg cggcaccaac gatcgtaaat tccgagcctt cgatgccaca
     4621 gatggcaaag tactgttcga ggttcccgcc acgtcaggcg tcaacggcgt gccgatgtcg
     4681 ttcgaagccg gcggcaa
//
LOCUS       DASWQN010001223         3338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2100501, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001223 DASWQN010000000
VERSION     DASWQN010001223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2100501"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VGC82_20765"
     CDS             complement(<1..855)
                     /locus_tag="VGC82_20765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0425762.1"
                     /translation="MAGSRLYNEYLEHYILADELGIDGIMLNEHHNAPFCMQARANIF
                     ASALAAATKNCKIVMLGNPLPAWDNPVQLSEELSMIDMLSKGRLVSGFVRGGGQEQLA
                     GGINPAFNRERFEEAHDLIVKAWTTPGPFRWEGTHYQQRVVNPWTVPLQKPHPRIWIP
                     GVISKETIIWAAEHGYPYIALNTPVERTRQIWDMYDAAAAKAGFKGGPDYHGLLKQIH
                     VAETEEKAVENARQFMWMQGEFTGLAHPVWSTPAGYGSPENRRAFVEFATGRSKSPRY
                     RPAVEKQME"
     gene            complement(1242..2882)
                     /locus_tag="VGC82_20770"
     CDS             complement(1242..2882)
                     /locus_tag="VGC82_20770"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0425763.1"
                     /translation="MNDRVEMARESSAKVATDFDAIVIGAGVSGLYQLYRLRELGLTA
                     RVFEAGSGVGGTWYWNRYPGARFDSESWTYGYSFSQELLDDWDWEEHFAGQPETERYL
                     NHVADKFDLRRDIQFKTTVTSSHYQEDSRSWKVTLDNGTSCTARFLITAIGVLSASVL
                     PRIPGVESFKGQSCHTHNWPKEPVSFAGKRVAVIGTGATGIQAITEIAKTAGHLTVFQ
                     RTAQWSAPLHNARVTPEEMRDIRKNYADIFARCQETVGCFIHTADPRSVFEVTEQERE
                     AFWEKRYGEPGFGIWMGNFRDVLTDREANKLMSDYLARKIRERVKDPAVAEKLVPKTH
                     GFGTRRVPLESGYFEVYNQPNVKLVDLTETPIERITPEGIRTSDADYQFDMIVYATGF
                     DAITGAFDQIDIRGVGGVSLKDKWANGPVTLLGILVDGFPNFMMVMGPHAGLGNFPRA
                     VEYTSDWVTALIRHARDNGVTRLDATPEAAKAWTDHVIETNEGLLFTEVDSWMTGINR
                     NLEGKQVRRVMRYSGGHPAFRERCEAVSAGGYRELSMA"
     gene            complement(3110..>3338)
                     /locus_tag="VGC82_20775"
     CDS             complement(3110..>3338)
                     /locus_tag="VGC82_20775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425764.1"
                     /translation="DDRVGETTRFTYRPNHEWYWFPQQKPTEVSMLKCYDSVTDGSVS
                     RWSFHTACIDPTAPADARCRKNVVVRSFVFF"
BASE COUNT          576 a         1072 c         1032 g          658 t
ORIGIN      
        1 ttccatctgc ttctcaactg ccgggcgata gcgcgggctt ttcgaccggc cggtggcaaa
       61 ttccacgaag gcacggcggt tttccgggct gccataaccg gcgggcgtgc tccagaccgg
      121 atgcgcgagg ccggtgaact caccctgcat ccacatgaac tgccgagcgt tttctacggc
      181 cttttcctcg gtctcggcga cgtggatctg cttcaacagg ccgtggtagt cggggccgcc
      241 cttgaaaccg gctttggcgg cagcggcgtc atacatatcc cagatctgcc gcgtgcgctc
      301 gaccggcgtg ttcagcgcga tgtaagggta gccgtgctcg gccgcccaga taatggtttc
      361 cttactgatg acaccaggaa tccagatgcg cgggtgcggc ttctgcagcg gcacggtcca
      421 gggattcacc acgcgctgct ggtaatgcgt gccctcccac cggaacgggc cgggggtcgt
      481 ccaggccttg acgatcaggt cgtgcgcttc ttcgaaccgc tcgcggttga aggcggggtt
      541 gatgccgccg gccagttgct cctgcccgcc gccgcggacg aagccggaca ccagccggcc
      601 cttggacagc atgtcgatca tcgacagctc ttccgagagc tgcaccgggt tgtcccaggc
      661 cggcagcgga ttgccgagca tcacgatctt gcagtttttc gtggcggcag cgagggccga
      721 ggcaaagatg ttggcgcggg cctgcatgca gaacggcgca ttgtggtgct cattcagcat
      781 gatgccatcg atgcccaact catcggccag gatgtagtgt tccaggtatt cattgtacag
      841 ccggctgccg gccaccggat cgtaatgctt gttggagaac atcagcgcgg tcgcgccgaa
      901 ctccagcccg gccttggcgt cataggccga catcggctgt tcagtgaaat acatcaggtg
      961 catgtgtgat ggttctccct ggtccgccgg gcgcgctgcg gctgctcgtt gctgtggata
     1021 ccaaagaggt tagcgccggg gcgaagagga ggcaaatccc caggtccggt ttgataacac
     1081 cccgctccac cgttactgcg cgccgtgcga actacatcgg gaacccgtcc actgtctccg
     1141 cggccactcc accaagcgcc tcgcccgccc gaggccggtc agctgccacc ggcctcgccc
     1201 gatggcgaca ttccgagtgc gtttccggtc agttctaact cctaggccat cgacagttcc
     1261 cgatagccac ccgccgaaac cgcttcgcag cgttcgcgga acgccggatg gccgccgctg
     1321 taccgcatta cccggcggac ctgtttaccc tccaggttgc ggttgatgcc ggtcatccag
     1381 gagtcgacct cggtgaacag caatccctcg ttggtttcga tgacatgatc ggtccaggct
     1441 ttcgcggcct cgggtgtcgc atccaggcgg gtcacgccgt tgtcgcgcgc gtgccggatc
     1501 agcgcggtga cccagtcaga ggtgtattcc accgcccgcg ggaagttgcc gaggccggcg
     1561 tgcgggccca tcaccatcat gaagtttggg aacccgtcga ccagaatgcc cagcagcgtc
     1621 actggcccat tggcccactt gtccttcagc gatacgccgc ccacgcccct gatgtcgatc
     1681 tggtcgaatg ccccggtgat cgcgtcgaac ccggtcgcgt agacgatcat gtcgaactgg
     1741 taatcggcgt ccgaggtgcg gataccctcc ggcgtgatgc gctcgatcgg cgtttcggtc
     1801 aggtcgacca gcttcacgtt cggctggttg taaacctcga aatagccact ttccaacggc
     1861 acccgccgcg tcccaaaacc gtgcgttttg gggaccagct tttccgcgac cgccggatcc
     1921 ttgactcgct cgcggatctt ccgcgccaga tagtcggaca tcagcttgtt ggcctcgcga
     1981 tcggttagga cgtcacggaa attgcccatc cagatgccga agcccggttc gccataacgc
     2041 ttttcccaaa acgcctcgcg ttcttgttcc gtcacctcga acacagagcg agggtcggcg
     2101 gtgtggatga agcagccgac ggtttcctgg catcgggcga agatatccgc ataattcttg
     2161 cggatgtcgc gcatttcctc gggcgtgacc ctggcgttgt gcaacggcgc cgaccactgt
     2221 gcggttcgct ggaacacggt caggtggccc gcggtcttgg caatttcggt gatggcctgg
     2281 atgccggtgg cgccggtgcc gatcaccgcg acccgcttgc ctgcaaaact gaccggctcc
     2341 ttgggccagt tgtgcgtgtg gcaggactgg cccttgaagc tctcgacgcc aggaatgcgc
     2401 ggcagaacag acgctgacag cacgccgatc gcggtgatca ggaatcgcgc ggtgcaagac
     2461 gtgccgttgt cgagcgtgac cttccagctt cggctgtctt cctggtaatg cgacgaggtc
     2521 accgtagtct tgaactggat gtcgcgccgg aggtcgaact tgtcggcgac gtggttcaga
     2581 taccgttcgg tttccggctg gccagcgaaa tgctcctccc agtcccagtc gtcgagcagt
     2641 tcctgggaaa aggagtaccc gtacgtccaa ctttcggagt caaaccgggc gccgggataa
     2701 cggttccagt accaggtgcc gccgacaccg gagccggcct cgaacacccg cgccgtcagg
     2761 ccaagttcgc gcaggcggta gagctgatag aggccggaga ccccggcgcc gatgacgatc
     2821 gcgtcgaaat cggtggctac cttcgcggac gactcgcgcg ccatttccac tctgtcgttc
     2881 atgcacacct ctccctgaga taagcctttc ggccctccta gttttttgag ccgctccgcc
     2941 gtcccatgtg ggactaagcg gccgaaggcc taaggcagga taatctggat cgtctcccac
     3001 ccgccaccca ccgccccatc gcaccgttgc gaggaatcga gcgtccgacg gccggcgcgg
     3061 agggatatga tcagtgccgc ccctccgccc gccacgtcct cccatcgccc taaaagaaga
     3121 cgaaggaccg gaccacgacg ttcttgcggc atcttgcgtc ggcaggcgca gtcggatcga
     3181 tgcaggccgt atggaacgac cagcgcgaga cggagccatc ggtcacggag tcatagcact
     3241 tgagcatcga gacttcggtt ggcttttgct ggggaaacca ataccactcg tggttgggcc
     3301 ggtaggtgaa gcgggtggtc tcaccgaccc ggtcgtcg
//
LOCUS       DASWQN010001224         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_778622, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001224 DASWQN010000000
VERSION     DASWQN010001224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778622"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /gene="soxC"
                     /locus_tag="VGC82_20780"
     CDS             complement(<1..992)
                     /gene="soxC"
                     /locus_tag="VGC82_20780"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR04555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HEY0425765.1"
                     /translation="MSNRTEPPEMHRVAGNGLLDRRFLLRAGGALAAGGGVATTAHAD
                     PLKVAQWMREPGAPFVPYGQPSHFESPVVRTYASLPGTTGTGSSRTPLHALEGMITPN
                     GLHFERLHNGIPDIDPEQHQLVIHGLVRRPLSFTIEALSRYPMVSRVAFIECGGNGGA
                     LYQPKPAQLGVQGLHGLLSCAEWTGVPLAVLLDEAGVDPRAKWLLAEGGDAASMSRSV
                     PLSKAMDDAIVALYQNGERVRPGNGYPMRLLLPGFEGNTQVKWLRRLMLIEEPMMTKD
                     ETSKYTITLPDGKSQQFVFPIEAKSVITRPSGGHQLVPGFHEIRGLAWSGRGRIA"
     gene            complement(1094..>1969)
                     /locus_tag="VGC82_20785"
     CDS             complement(1094..>1969)
                     /locus_tag="VGC82_20785"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0425766.1"
                     /translation="SSVVALDVATGKIKGHHQYHPNDSWDWDEVSPPIIVDYQHNGKS
                     IKGLVDVARDGYLWMLERTPDKINFVAGQPFVYHNTFTDLDLKTGRPNVADEHKPGTG
                     KLADFCPSLWGGKDWPPVAFSPKTKLLYIPANENLCSTILGRPVKYVAGQPFTGAAST
                     LYLHKGADHIGELQAWNLDTGKKVWTHDFGMSPNWGPVLATAGNVLFAGGTNDRKFRA
                     FDATDGKVLFEVPATSGVNGVPMSFEAGGKQYIAVQSGWGVDATRMQSRLNLVRPGQY
                     PEVPQGGAIWVFALD"
BASE COUNT          353 a          626 c          612 g          378 t
ORIGIN      
        1 gcgatgcggc cccggcccga ccacgccagc ccgcggatct cgtggaagcc cggcaccagc
       61 tggtggccgc cggacgggcg ggtgatcacc gatttcgcct cgatcggaaa cacgaattgc
      121 tgactctttc cgtcgggcaa cgtgatcgta tatttcgatg tttcatcctt tgtcatcatc
      181 ggttcttcga tcaacatcag gcggcggagc cacttcacct gcgtgttgcc ttcgaaacca
      241 ggcaacagca gacgcatggg ataaccgttg ccgggacgca cgcgctcccc gttctggtac
      301 aacgcgacga tcgcgtcatc catggccttg gacaacggca cgctgcggct catgctggcc
      361 gcgtcgccgc cttccgccaa tagccacttt gcccgtgggt cgacacctgc ctcgtccagc
      421 aggacggcca gcggcacgcc ggtccattcg gcgcacgaca gcaggccgtg cagcccttgc
      481 acgccgagct gcgcgggctt cggctgatac agcgcgccgc cattgccgcc gcactcgatg
      541 aacgcgaccc tggacaccat cgggtagcgc gacagggcct cgatcgtgaa actgaggggg
      601 cgccgcacca ggccgtggat gacaagttgg tgctgctccg gatcgatgtc agggatgccg
      661 ttatgcagcc gttcgaagtg caggccgttg ggcgtgatca tgccctccag cgcatgcaac
      721 ggggtgcggg acgacccggt tccggtcgtt cctggcaagc tggcataggt gcggaccacc
      781 ggggattcga agtgcgaggg ttgcccgtat ggcacgaacg gcgcacccgg ttcgcgcatc
      841 cactgcgcca ccttcagcgg atcggcgtgc gccgtggttg cgacaccgcc gccggcggca
      901 agtgcgccgc cagctcgcag caggaaacgg cggtcaagca gaccattgcc ggcaacccga
      961 tgcatttcag ggggttcggt gcgattcgac atccgtcgtt tcccttgttc cggttcgggc
     1021 acggcacgct gggcgaaatc gcgtcgcgaa acgggctgcg cgacgaggat tggcgcggcc
     1081 cgcgcgcaac gatctaatca agtgcgaata cccagatcgc gccaccttgc ggcacctccg
     1141 gatactgacc gggccgcacc aggttcaagc ggctttgcat gcgcgtggcg tccacccccc
     1201 atccggactg cacggcgata tactgcttgc cgccggcttc gaacgacatc ggcacgccgt
     1261 tgacgcctga cgtggcggga acctcgaaca gtactttgcc atctgtggca tcgaaggctc
     1321 ggaatttacg atcattggtg ccgcccgcga acagcacatt tccggcggtg gcgaggaccg
     1381 gtccccaatt cgggctcatg ccgaagtcat gcgtccagac cttcttgccg gtatcgaggt
     1441 tccaggcctg caattcgccg atatgatccg cgcctttgtg caagtagagc gtgctggccg
     1501 cgccggtgaa tggttggcca gcgacgtatt tgactggacg gcccaggatc gtagagcaga
     1561 gattttcgtt tgcgggaata taaagcaact ttgtcttggg gctgaacgcc accggcggcc
     1621 aatccttgcc gccccatagg ctggggcaga aatcagccaa tttcccggtc cccggtttgt
     1681 gttcgtcagc tacattcggc cgccccgtct tcagatcgag gtcggtgaac gtattatggt
     1741 aaacgaacgg ctgccccgcg acgaagttga tcttgtctgg ggtacgctca agcatccaca
     1801 ggtaaccgtc gcgagcgaca tcaaccagac ctttgatcga cttgccgttg tgttggtagt
     1861 caacgatgat cggcggcgag acctcgtccc agtcccacga atcgttgggg tgatactggt
     1921 ggtgtccctt gatcttgccg gtagcgacat ccagcgccac gaccgacga
//
LOCUS       DASWQN010001225         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2495021, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001225 DASWQN010000000
VERSION     DASWQN010001225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2495021"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1155)
                     /locus_tag="VGC82_20790"
     CDS             complement(241..1155)
                     /locus_tag="VGC82_20790"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0425767.1"
                     /translation="MNSIADCLIIGAGPAGLTAAIYLARFRLTVAVIDNGKSRAGLIP
                     CTHNHAGFPEGISGKELLARMRRQASRYGVVVQEGQVAGLRLRDDLFEADGAGMPTRT
                     RTVLLATGVTNRRPIMPDSVHDAALAEGRLRYCPVCDGYEVSDQRVAVIGTAGRGLKE
                     AEFLRSYTRDVTLVAGDGPHFLSEADRRRIAAIGISTIDGPVRDFALTRQGLRFTAAN
                     GQLEFASVYPALGSEIHSGLAAALGADCTDEGCIRVDAHQRTSVPRLYAAGDVVLGLD
                     QISHAMGDAGVAATTIRNDLAARSPLLR"
     gene            complement(1523..2314)
                     /locus_tag="VGC82_20795"
     CDS             complement(1523..2314)
                     /locus_tag="VGC82_20795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0425768.1"
                     /translation="MEHWKPEPLDPGLIQLHRRGKGKPLVMIHCLGQSWRLWDVLDPL
                     MDQNELIAYSLPGHPGTPLPGHQYGVPELTEQLRQVAVREGLTKFHLIGISLGGSVAQ
                     HLAGTYPEMVDKLVLMDCSPRYDDEARANWPIRAAAARKDGVKSLIPMLLQVFFTADS
                     IAENGPNVRHVRETFEACDGEGYALACEALATLDAREQTRKVTAATLIMLGPTNASRS
                     RTRHNGCMKRSPAASWLKSPSRATRRCGSVLNSLLGNCEHSWTRA"
     gene            complement(2364..3197)
                     /locus_tag="VGC82_20800"
     CDS             complement(2364..3197)
                     /locus_tag="VGC82_20800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0425769.1"
                     /translation="MTDRVAIVTGGLRGLGRGMTLGLAKSGVRVLAVGHLPEDVAAIE
                     GTSNVRAMVADLRSPAECDRVVTEALSAFGRIDILVNNAGLTFTYIDPDRFRREKPRK
                     FWEVGDEVVQNVMATNYVAADQMGRRVALILVRQGWGRIVNVTTKLDTMNKEGSLPYG
                     SSKAALEMATEVWAKEVAGTGVTVNIVNPGAGANTPGMNQEMRDWSREGRAPRLVEPD
                     EMVPPLLFVVSTAADNVNGYRFDALTWDVSLPPAEAARRNGRPAGFVLHPQDESAFPA
                     R"
     gene            complement(3205..>3569)
                     /locus_tag="VGC82_20805"
     CDS             complement(3205..>3569)
                     /locus_tag="VGC82_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425770.1"
                     /translation="PAQVGVARAFFVADTAEEKAAAVERRITAQRRLHAISQRPDGNN
                     TASIMAFSDTREASEESALYGTPDEICRKIELMQAQGVEYLLLNGGGTSRDNLRRFAK
                     EVMPHFRTEAPARTAAAE"
BASE COUNT          623 a         1157 c         1099 g          690 t
ORIGIN      
        1 tatgccccga ttctagcgcc cgtttccgcg tcgcgttaag tccgacaggc tgctagagca
       61 gtttcacggt gactggagac agaggttctc tcgcggctgc aaaggaagtc gtgggtggcc
      121 gcgccaagca cggccatgac acataggcgt caggcggcga acccgccggc accttttcca
      181 gtcaggcgtg aaaacgccca aacctggctg atgcgtcagg tcgatcgcgg tgacgcaatt
      241 tcagcgcaga agcggcgaac gggcggcaag atcgttccgg atcgtcgtcg ctgccacacc
      301 ggcatcaccc atggcatgac tgatctggtc gaggccgaga acaacgtcac ccgccgcata
      361 aagccttgga acgctagtac gctgatgggc atcaacccgg atgcagcctt cgtccgtgca
      421 gtccgcccca agggcagcgg caaggccaga atgtatttcg gaaccaagtg cgggatagac
      481 gcttgcaaac tccagctgtc cattcgccgc cgtgaagcgg aggccttgcc tggtcagggc
      541 gaagtcgcgg acaggcccat cgatcgttga aatgccgatg gcggcgatac ggcgacggtc
      601 cgcctcgctg aggaaatgcg gaccgtcgcc agcgaccagt gttacatccc ttgtgtagga
      661 ccgcaggaat tccgcttcct tcaacccccg ccctgctgtg ccgatgaccg cgacacgttg
      721 gtcggagacc tcgtagccgt cgcaaaccgg gcagtaccgc agccgacctt cggccagcgc
      781 tgcgtcatgg acgctgtcag gcatgatagg gcgccggttt gtcacgccgg ttgccagcag
      841 cacagtgcgc gttcgggttg gcatacccgc gccatcagct tcgaaaagat cgtcacggag
      901 acgcagtccg gccacctgcc cttcctggac tacgaccccg taccgcgatg cctgtcggcg
      961 catcctggcg agcagttcct tgcccgaaat cccctcgggg aagcctgcat gattgtgcgt
     1021 gcatgggatc agcccggcgc ggctcttgcc gttgtcgatg accgccacgg tcagacggaa
     1081 cctggccaag taaatggcgg cagtcagtcc ggccggaccc gcaccgatga tcaggcaatc
     1141 ggcgatgctg ttcacaaagc ctacctctcg atgtggacgt tggtgtcgcg cgccgggtta
     1201 aagggcggcg gcgtgccaac aacgactctt atactagcgg ccgttggcct gcccttgcag
     1261 gtcaacttca aggccgtcgg tataatagcg tccaggtcgg atcggtttgc cgcacatgta
     1321 tagcacgtcg tcgtgacatg gagtgaatca ggacacgaac cctcctgagt ctctggcttg
     1381 tgccgcccgc gatgctcccg gaccccgacc tgttgcctcg agcggataga ccatttccag
     1441 cctggactgt acaggccgaa attcgtctat ttctttgttt gatcgcatga ttcgcacgct
     1501 tcggacgttc cgcccagcgt aatcacgctc tagtccagga atgctcgcag ttgcccaaca
     1561 acgaattcag gacgctcccg caccgacgcg tggcccgcga gggggacttc aaccagcttg
     1621 ctgccggcga tcgtttcatg catccattgt gccgcgtcct tgaacgactg gcgttcgttg
     1681 gacccagcat gatcagggtt gccgcggtta ctttcctggt ctgctctctg gcgtcgagcg
     1741 ttgccagcgc ctcgcaggcc aacgcgtagc cttcgccgtc gcaggcctcg aacgtttcac
     1801 gtacatgacg gacattaggg ccgttctcgg cgatcgaatc cgccgtgaag aagacctgca
     1861 acagcatggg gatcaggctc ttgacaccat ctttgcgtgc cgccgctgct cgtatcggcc
     1921 agttcgcccg agcctcgtcg tcgtagcggg gggaacagtc catcagtacg agtttgtcca
     1981 ccatttcggg gtaggtgccg gcgagatgct gggccacgct gccgcccagc gagataccga
     2041 tcaggtggaa cttcgtcagt ccctcccgta ccgcgacctg tcgcagttgt tcggtgagtt
     2101 ccggcacgcc gtactggtgt ccgggcaggg gcgtaccggg atggccgggc aggctgtagg
     2161 cgatcagctc gttctggtcc atcagcgggt cgagcacatc ccaaagccgc caggactgac
     2221 ccagacagtg gatcatcacc aaaggcttgc ccttgccgcg ccggtgaagc tggatcaggc
     2281 ccggatcaag cggctcgggc ttccagtgtt ccatgcggtt ggtccttttg cttcagggga
     2341 aaccaagcta acggcgctgc catttaccgc gccggaaatg cgctctcgtc ctgcggatgc
     2401 aacacgaatc ccgcggggcg gccgttgcgg cgggccgctt cggcgggtgg caacgaaacg
     2461 tcccaggtca atgcatcgaa acggtagccg ttcacgttgt ctgccgccgt tgaaaccacg
     2521 aacagtagcg gcggcaccat ctcgtccggt tccaccagcc tgggcgcgcg gccctcgcgg
     2581 ctccagtctc gcatttcctg gttcatgccg ggggtgtttg cgccggctcc gggattgacg
     2641 atgttcaccg tgacgcccgt gccggcgact tccttggccc agacctcggt cgccatctcc
     2701 agcgccgcct tggacgagcc gtacggcagg ctgccctcct tgttcatggt gtccagcttg
     2761 gtggtgacgt tgacgatccg gccccaaccc tgccgcacca ggatcagtgc cacgcggcgc
     2821 cccatctgat cggctgcgac gtagttggtg gccatcacgt tctggaccac ctcgtcgccg
     2881 acctcccaga acttgcgcgg cttttcacgg cgaaaccggt ctggatcgat ataggtaaag
     2941 gtcaggccgg cattgttcac caggatatcg atgcgcccaa acgccgacag cgcttcggtc
     3001 acgaccctgt cgcactccgc cggggaacgg aggtcggcga ccatggcgcg cacgttcgag
     3061 gtgccctcga tggcagcgac atcttccggc agatgcccga cggccagcac ccgcacgccg
     3121 gacttagcca gccccagggt catgccccgt cccaaaccac gaagcccgcc ggtaacgatc
     3181 gcgaccctgt cggtcatggc gccgctactc cgcggccgcg gttcgggcag gtgcttccgt
     3241 ccggaaatgc ggcatcacct ccttggcgaa acggcgcaga ttgtcgcgtg aggtgccgcc
     3301 gccgttcagc agcagatact cgacgccctg cgcctgcatc agttcgatct tgcggcagat
     3361 ttcgtcgggc gtgccataca gcgcgctttc ctcgctggct tcgcgggtgt cggagaaagc
     3421 catgatgctg gctgtgttgt taccgtccgg gcgttggctg atggcatgca gacggcgttg
     3481 cgcggtgatc cgccgctcta ccgccgcagc tttctcctca gcggtgtctg cgacgaagaa
     3541 ggcgcgggcc acgccgacct gagcgggat
//
LOCUS       DASWQN010001226         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2100629, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001226 DASWQN010000000
VERSION     DASWQN010001226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2100629"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..627)
                     /locus_tag="VGC82_20810"
     CDS             complement(121..627)
                     /locus_tag="VGC82_20810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425771.1"
                     /translation="MLAPSTAHAGAPEELVGLLIQGCLPFTGNPPALRDWAKRTGLPP
                     VPEQARIAFLHGAPGQAFDASAAEAKLVLVSSDDGICSAVTDRATQQAVGDALEAGFR
                     QAGLAFRLVIERDDARVSAIHNREYLATKNLATKTLATKNKIGWRILAATVKGDTGGE
                     AMLTAAPE"
     gene            complement(657..1433)
                     /locus_tag="VGC82_20815"
     CDS             complement(657..1433)
                     /locus_tag="VGC82_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0425772.1"
                     /translation="MSEQIPKIRIRGLYKRFGVKRVLDGVDLDVPRGTSLVVIGGSGS
                     GKSVLLKCVLGLIEPDDGIIEIDGKDILRLPRGERELARSRIGMLFQAGALFDSLPVW
                     ENVAFGLLASHKATRADARSQADNYLGQVGLAASVGDLSPAELSGGMQKRVALARAIA
                     SQPDIMFFDEPTTGLDPIMGAVIDGLIVDCVKRLGSTAIAITHDMASARRIGDQAAML
                     LNGRIIWSGPAENLMNSGKAEVDQFTHGRRDGPIQMELRR"
     gene            1616..2038
                     /locus_tag="VGC82_20820"
     CDS             1616..2038
                     /locus_tag="VGC82_20820"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0425773.1"
                     /translation="MNDVVGTTETTGGKLAHATLENANYVAGRRSFFKYRDLGVTDAT
                     DGKMRAQVTSATQGLGQPTGWHYHVCEHQLVYMLKGWVDLEFEDGRKIRLMPGESLMI
                     PGGMRHNETGTADELELLEVSVPAEMKTVVCDPPSGRG"
     gene            2192..>3548
                     /locus_tag="VGC82_20825"
     CDS             2192..>3548
                     /locus_tag="VGC82_20825"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0425774.1"
                     /translation="MMLAFTIARRELRGGVRGLWIVLACLALGVGVIAAVGTLRAAVD
                     AGLAADARALLGGDLEIDGGSQPLPQRLADWLQARGATTSAVTQMRSMLVGPSGERQL
                     IELKAVDAMWPLVGEPTLAPAQPLRGALRQRDQQYGLLAEQVVLDRLGLHPGDTARLG
                     TATFRVAGALTREPDHVATPSILGPRVLISADALPSTGLISPGAMVRYAIRLTAPDPA
                     AITTGIRTEFPNQGWRIRDPSEAAPGVSRFIEQTALFLTLVGLTSLLVGGIGVANGVR
                     AWLDARARTIATLRCLGASARLVFAVCLIQVLTLSAAGVIAGVAAGAALPLIGARFLA
                     PILPVPPVLGLYPVPLMLAALYGFLIALCFAIWPLGRAARIPGGALFRDGLIPETVRP
                     SGTLIAVNAMFAGALVALTVATATDRWFALYFCVAAGLTLALFHAGGAAVTRLARLAP
                     VL"
BASE COUNT          539 a         1183 c         1173 g          653 t
ORIGIN      
        1 gaactgcccg tcgatccatc gacaaagcca ccagtacccg gcgatagcct gtacttgttc
       61 ggccaagccc gttgagtgtc ttacggttcc cgttgagtgt ttcgcgggtt gttgccaagt
      121 tcactccggc gccgcggtca gcatggcctc gccgccggta tcgcccttca ccgtcgcggc
      181 caggatgcgc cagccgatct tattcttcgt cgccagggtc ttcgtcgcca ggttcttcgt
      241 cgccagatat tcgcgattgt gaatcgcgct gacacgggca tcgtcccgct cgatcaccaa
      301 acgaaaagcc aagcccgcct gccggaagcc ggcctccagc gcatcgccca ccgcctgctg
      361 cgtcgcccgg tcggtaaccg ccgaacaaat gccgtcgtcc gaggaaacca gaaccagttt
      421 cgcctcggcg gccgaggcat cgaaggcctg gcccggcgca ccatgcagga atgcaatgcg
      481 agcttgttcc gggaccggag gcaggccggt tcgctttgcc caatcccgca acgccggcgg
      541 gttgcccgtg aacggcaaac agccctggat caacaggcca accagctcct cgggcgcgcc
      601 ggcatgtgcc gtgcttggtg ctagcactgc cgccgccagc agcacgaccg cccgcttcag
      661 cgccgcaact ccatctggat cggaccgtcg cgccggccgt gggtgaactg atcgacctcg
      721 gccttgccgc tgttcatcag gttttcggcc ggtcccgacc agatgatgcg gccgttcagc
      781 agcatcgccg cctgatcccc gatccgccgc gcactggcca tgtcatgggt gatggcgatg
      841 gcggtgctgc ccaggcgctt gacgcaatcc acgatcagac cgtcgatcac cgcgcccatg
      901 atcgggtcga ggcccgtggt cggctcatcg aagaacatga tatccggctg cgaggcgatc
      961 gctcgggcca gcgcgacgcg cttctgcatg ccgccggaca actccgccgg agacaggtcg
     1021 ccgacactgg cagccaagcc cacctgcccc agataattgt ccgcctggga gcgggcatcg
     1081 gctcgagtcg ccttgtggct ggcgagcagg ccgaatgcga cgttctccca gaccggcagg
     1141 ctgtcgaaca gggcaccggc ctggaacagc atgccgatac ggctgcgggc caattcccgc
     1201 tcgccgcgcg gcagccgcag gatgtccttg ccatcgattt cgataatgcc gtcgtccggc
     1261 tcgatcaggc cgagcacgca tttcagcagc accgatttgc cggagccgga gccgccgatg
     1321 acgaccagcg aggtccctcg cggcacgtcc aggtcaaccc cgtcgagtac ccgcttgacg
     1381 ccgaagcgct tgtacaggcc gcggatgcgg attttcggta tctgttcgct catgcttgct
     1441 tgatggcgcg ggacgcggat ttcgacaagg tcggatgatg tgggtcgggt atcgtgggcc
     1501 gggtgccgtg gaccgggtat cgtgggttgc ggctggtcgg agatgggatt tagctgtatt
     1561 ggttcgcgcg ccgccggcga caaggcgtga cgcgcgccgc aacaggagaa caacgatgaa
     1621 cgatgtcgtg gggacgaccg agacgactgg cggcaagctg gcccatgcga ccttggagaa
     1681 cgcgaattac gtcgcgggac ggcgatcctt tttcaaatat cgcgacctgg gcgtcaccga
     1741 tgcgaccgac ggcaagatgc gggcgcaggt gacttcggca acgcaaggcc tgggtcagcc
     1801 gacgggatgg cactaccacg tctgcgaaca ccagcttgtg tatatgctga agggctgggt
     1861 tgacctggaa tttgaggatg gccgcaagat ccgcctgatg ccgggtgagt cgctgatgat
     1921 cccgggcggg atgcgccaca acgaaactgg gacagccgac gaactggaac tgctggaggt
     1981 ctcggtcccg gccgaaatga agacggtggt atgcgatcca ccttcgggaa gaggataatc
     2041 cgactggttg gaggccccgc aagccaccct gaaatagccg cttcgagcgc ggtcctggat
     2101 accgtcaagg tggggaagtt cgcccggtcc gaagcggttt tttatcaccg ggcacttggg
     2161 aggcgccccg accgctccac gttgcaatga catgatgttg gccttcacca ttgcccgccg
     2221 agagctgcgg ggcggcgttc gcgggctttg gatcgtgctg gcctgcctgg ccctcggcgt
     2281 cggcgttatc gcggcggtcg gaacattgcg agcggcggtt gacgccgggc tcgccgccga
     2341 cgcccgcgcc ctgctggggg gcgacctcga gatcgacggc gggtcccagc cattgccgca
     2401 acggttggcc gactggctcc aggcgcgagg tgccacgacg tccgccgtga cgcagatgcg
     2461 ctcgatgctg gtcgggccgt ccggcgagcg gcaattgatc gaactcaagg cggttgacgc
     2521 catgtggccg ctggttggcg agcccaccct tgcccccgcc cagccgctcc ggggcgcttt
     2581 gcggcagcgc gaccaacaat acgggcttct ggccgaacag gtcgtgctcg accggctcgg
     2641 tcttcatccc ggcgataccg cgcggctcgg caccgctacg ttccgcgtgg ccggcgcgtt
     2701 gactcgcgaa cccgatcacg tcgcgacgcc atccatcctc ggcccgcgtg tcctgatctc
     2761 ggccgacgcg ctgccttcga ccggcttgat ctcccccggt gcgatggtgc gctacgcgat
     2821 caggctgacc gcgcccgatc cggccgccat cacgactggt atccgaacgg aattccccaa
     2881 ccagggctgg cgcattcgtg atccgtccga ggcggctccc ggtgtcagcc ggttcatcga
     2941 acagacggct ctgttcctca cccttgtcgg cctgacgtcg cttttggttg gcggcatcgg
     3001 cgttgccaac ggcgtgcgag cctggctcga cgcgcgcgcc cgaaccatcg ccacgcttcg
     3061 ctgtctcggc gcctccgccc gtctggtctt cgccgtttgc ctgatccagg tgctgacgct
     3121 gtccgccgcg ggcgtgattg ccggcgtggc tgccggtgcg gccttgccgc ttatcggcgc
     3181 ccgcttcctg gcaccgatcc tgccggtgcc gccggtcctc gggctttacc cggtgccgct
     3241 gatgctggcc gcgctgtatg gtttcctgat cgcgctttgc ttcgcgatat ggccgctggg
     3301 ccgagcggct cgaattccgg gcggtgcgct gttcagggat gggctgatac cggaaacggt
     3361 gcggccctcc ggtacgctga tcgcggtcaa cgcgatgttc gccggcgcgc tggtcgcact
     3421 gacggtggcc actgcgaccg atcgatggtt cgccctgtat ttctgcgttg ccgccggcct
     3481 gacgctggcg ttgttccacg ccggcggggc ggcggtcacg cgccttgcgc ggctggcgcc
     3541 ggtgttgc
//
LOCUS       DASWQN010001227         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1440950, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001227 DASWQN010000000
VERSION     DASWQN010001227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440950"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..575
                     /gene="imm40"
                     /locus_tag="VGC82_20830"
     CDS             222..575
                     /gene="imm40"
                     /locus_tag="VGC82_20830"
                     /inference="COORDINATES: protein motif:HMM:NF026907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm40 family immunity protein"
                     /protein_id="HEY0425775.1"
                     /translation="MPKNFSTVVESILSRGIDMEEFGSANWALCPEDALEALSKLHEL
                     GIPVLGGDVWEKRDGHPSLTLDNWFSRPKTDEPFAGFLERSIAEARNYIRSYRPSGNV
                     EYLFEIVVDRSAGLS"
     gene            886..2172
                     /gene="murA"
                     /locus_tag="VGC82_20835"
     CDS             886..2172
                     /gene="murA"
                     /locus_tag="VGC82_20835"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY0425776.1"
                     /translation="MVEGGHKLSGTIEPSGNKNAALPIIAAALLTEHPVTLKNVPRIR
                     DTETLVELIRSVGASAEWQQRNTLAIHAKSIHATALDPVLCARIRASILLAGPLLARC
                     GGVTLPPPGGDVIGRRRLDTHFLAFEQLGATVVATDRLEFRAARLKGADVFLDEPSVT
                     ATENALMAAVAAHGTTYLRNVASEPHVQDLANFLVALGARIEGIGTNTITVHGPATLG
                     GASFSIGPDHIEVGSLIGLAAVTRSPLRIARAGGAHLRSLRMGFERLGIICEVEGDDL
                     IVPANQTLKIQDDFGGHVPKLEDQPWPAFPADLMSIAIVTATQCDGVILMFEKMFESR
                     MFFVDKLISMGARIVLCDPHRAIVAGASRLRGAPMTSPDIRAGMAMLLAAACAKGVST
                     INNADQIERGYERIDQRLNALGARITRVPARTAESE"
     gene            complement(2383..>2665)
                     /locus_tag="VGC82_20840"
     CDS             complement(2383..>2665)
                     /locus_tag="VGC82_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425777.1"
                     /translation="RGGAASPGPVVVFICIPAAIWPCIVAFRWRHYSEFFRDNIGRPL
                     DPLWSFIGGSSEPPKMNFNRLLLVVNIGWCLFCSIPLWMMLGGCTSALG"
BASE COUNT          560 a          809 c          790 g          506 t
ORIGIN      
        1 ggagaaaatc gaggcaaaga catggaacta agcggggcct ccatagccga tcaagacttt
       61 atcgcacagg ccagggagga tattcctcga ttgctggagg aaattgcgag gctaaagaga
      121 tgcatcgttg aagactgatt ctcaatgcgg aaagctgccg gcggcaaatt cgcatcaccg
      181 gcctgttcgg cggtaatctt tagacctagc ggaaactaac aatgccaaag aacttttcta
      241 cggttgttga atccatatta tctcgtggga tcgacatgga agaatttgga agcgccaact
      301 gggcgctctg tccagaggat gcactagaag ctctctcaaa acttcacgaa ttgggaatac
      361 cggtactcgg cggagatgtc tgggagaaga gggacggcca tccgtctctt actctcgata
      421 actggttttc tcgcccaaaa acggacgagc ctttcgcggg gtttttagaa agaagtattg
      481 cggaagcccg caattatatt cgaagctatc gtccttcagg aaacgtcgag tacctctttg
      541 agatcgtggt tgatcgatcg gcggggttga gctgaacccg ctccttcttg ggtgggcttc
      601 ctaccattcc acctcccaaa tggaactgaa aaattgagaa attgggaact ctcaatgttc
      661 ctgctcttgc aggcgatggt cattcgacct ggatcttgga ccagcttgca aaagctggaa
      721 catcaaggcg ggatgccttc gcaagtcatg aattcgagag tggatgcgtc ggccgcctgt
      781 gctacggtgc atttcagaac ccactgattc cacaccattt aacccaagca tcgggtgaaa
      841 cccatgagtg acccgacagc cgggccggcc tcccctatcc agtacatggt cgaaggcgga
      901 cacaaattgt ccggcacgat cgaaccctcc ggaaacaaga acgccgcgct gccgatcatc
      961 gcggcggcat tgctgaccga gcatcccgtc acgctgaaaa atgtccctcg cattcgggac
     1021 accgaaaccc tcgtcgagct cattcgctcg gtcggcgcgt cagccgaatg gcagcagcgc
     1081 aacacgctcg cgatccacgc caagagcatc cacgccaccg ctctcgatcc ggtgctgtgc
     1141 gcgcggatcc gcgcctcgat cctgctggca gggccgctgc tggcccgctg cggcggagtg
     1201 acgctgccgc cgcctggcgg cgacgtcatc ggccggcggc gtctcgacac gcatttcctg
     1261 gcgttcgaac agctcggggc caccgtcgtc gccaccgacc ggctggaatt ccgggcggcg
     1321 cgcctcaagg gcgcggacgt cttcctcgac gagcccagcg tgacggccac cgagaacgcg
     1381 ttaatggcgg cggtcgccgc ccatggcacc acctatctgc gcaatgtcgc ttcggagccg
     1441 catgtgcagg acctcgcgaa tttcctggtt gcgcttgggg cgcggatcga aggcatcgga
     1501 accaacacga taacggtcca cggacccgcg acgctcggcg gcgccagctt ttcgatcggg
     1561 cccgatcaca tcgaagtcgg ctcgttgatc gggctggccg cagtgacccg ttcgccgctc
     1621 cgcatcgcgc gcgccggggg tgcgcatctg cgctcgctcc ggatgggttt tgaacggctc
     1681 ggcatcatct gcgaagtcga gggcgacgac ctgatcgtgc ccgccaatca gacgctcaag
     1741 atccaggacg atttcggcgg ccacgtgccg aaactggaag atcagccctg gccggccttt
     1801 ccggccgatc tgatgtcgat cgccatcgtg accgcgaccc agtgcgacgg cgtcatcctg
     1861 atgttcgaga agatgttcga atcccggatg ttcttcgtcg acaagctgat atcgatgggc
     1921 gcccgcatcg tgctgtgcga tccgcaccgg gcgatcgtgg cgggagcgag ccggctgcgg
     1981 ggcgcgccga tgacatctcc cgatatccgg gccgggatgg ccatgctgct ggccgcggcc
     2041 tgcgccaagg gcgtcagcac catcaacaac gccgaccaga tcgagcgcgg ctatgaacgc
     2101 atcgaccagc ggctcaatgc gctgggcgcg cggatcaccc gcgttcccgc gcggaccgcc
     2161 gagtccgaat agaccctggt ggctgcggtt tggtcaccgg ccgcgccctt cgcgcggcct
     2221 gatggttttg cgtggccccc agaccagggc gtgaacccat aaatttggaa tgatcggccg
     2281 acgtccgcct tggtgcgcat aacggacttt agtcgggcat cgcaccgtgt ccgttttgtg
     2341 ccagaagcgg tcatggccaa gcaatcgacc ttttgctgct gcttaaccta gtgcgctagt
     2401 gcacccaccc agcatcatcc aaagcggtat cgaacagaac aggcaccagc cgatatttac
     2461 gaccagaaga agacggttga aattcatctt cggcggctca gaggagccgc caatgaaact
     2521 ccacagcgga tcgagtggtc ttccaatgtt gtcacggaaa aattcggagt agtgccgcca
     2581 acggaaggcc actatgcacg gccaaatggc ggccggaata cagataaaaa caacgacagg
     2641 ccccggcgag gcagctcctc cgcgg
//
LOCUS       DASWQN010001228         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2363402, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001228 DASWQN010000000
VERSION     DASWQN010001228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2363402"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..759
                     /locus_tag="VGC82_20845"
     CDS             172..759
                     /locus_tag="VGC82_20845"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0425778.1"
                     /translation="MFAVIFEVNPRPEAWDAYLGHAATLRPELLAIDGFIANERYASL
                     TRSGWLVSLSIWRDEKALIRWRTHALHHDIQGKGRSAVFADYHLRVGEIVADTNLDSV
                     PPRQRFDTTEVADMKVVTLAERPLGAVPVAPPTDGDVFDSITRPGNGLYLSVWPDLPS
                     ARIAMEDMTGHRRIVQIIRDYGMFARAEAPQYFPP"
     gene            756..2231
                     /locus_tag="VGC82_20850"
     CDS             756..2231
                     /locus_tag="VGC82_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0425779.1"
                     /translation="MTIPPEQEDVVRFLAGLAGAPPKETHISAVFVGTDTVWKLKKAV
                     RLPFLNFTTCDAREHFLRRELELNKPAASGIYRDVIGIERDTGQALRFGDDHAIDFVL
                     RMAPVPSQDFLDVIASDNRLTAELLDDLGDAVSACHGKLPPLLGRDFAATMRDITQGN
                     LRSALAAGLPEPDVTAWQAQINEAIDGLVPWMAERSSAGYVRRCHGDLHLGNLCLWEG
                     KPVPFDALEFDEALATIDVGYDLAFLLMDLDQRVGRAAANRVMNRYIARTGDARIMAG
                     LPVFLSQRAIIRAHVLAAMGQDGAAYLTAARRYLAPAEPAVIAIGGLQGTGKSTLARA
                     LAPELGRPPGALIVRSDEIRKRLFGLHPQQRLQQESYGPDANIATNSALIEQTRVAAE
                     AGHTAIVDATFLDIETRRDLKAAVGSTGVRFLGIWLQAPLALLEQRVAARTGDASDAT
                     VAVLRRSAGNDPGPVDWLAVDASDATRALASVRQALLNALT"
BASE COUNT          394 a          764 c          710 g          409 t
ORIGIN      
        1 ggcgtacgga gcggatccga ggaaacttgg cctggatcac tgagcgatgc cgaccatgcg
       61 gatggcactt cggccccagc cgaattgatg aacatggaca gcaccgcctc ccgttctcat
      121 gatccctgcc atgaaaccac cgctttcctg acccaacgga gggactaatc gatgttcgcc
      181 gtgatcttcg aagtaaaccc gcgtcccgag gcatgggacg cctacctcgg ccacgcggcg
      241 accttgcggc cggagttgct ggccatcgac gggtttatcg ccaatgaacg ctatgccagc
      301 ctgacccgtt ccggctggct ggtttcgctg tccatctggc gcgacgagaa agccttgatc
      361 cgttggcgca cccacgcgct acaccacgat attcagggca aaggccgatc ggccgtgttc
      421 gccgactacc atctccgtgt cggcgagatc gtcgcggaca ccaatttgga cagcgtgccg
      481 ccgcgccagc gctttgacac caccgaagtc gccgacatga aggttgtcac cctggcggaa
      541 cggccactag gcgccgtgcc ggtagcgccg cccactgacg gcgatgtctt cgacagcata
      601 acccgtccgg gcaatggcct gtatctgagc gtttggcctg atcttccgtc cgcccgtatt
      661 gccatggagg acatgaccgg acatcggcgg atcgtccaga tcatccggga ttacggcatg
      721 ttcgcccgcg ccgaagcgcc gcaatacttt ccgccatgac gattccgccg gagcaggagg
      781 atgtcgtgcg ctttctcgcc ggtctggccg gcgccccacc gaaggaaacc cacatctccg
      841 cggtcttcgt cggcacggat acggtctgga agttaaagaa ggcggtgcgg ctgccgtttc
      901 tcaatttcac cacctgcgat gcgcgcgagc acttcctgcg ccgcgaactg gaactcaaca
      961 aaccggcggc gagcggcatc tatcgcgacg tgatcggaat cgagcgcgac accggccagg
     1021 cccttcggtt cggtgatgac cacgcgatcg atttcgtgct tcgaatggct ccggtgccca
     1081 gtcaggattt cctcgacgtg atcgcgtcgg acaatcggct gacggcggag ctcctggatg
     1141 acctcggcga cgcggtgtcg gcctgtcatg gcaagctgcc gccgctgttg gggcgcgatt
     1201 tcgccgcgac catgcgcgac atcacgcagg gcaacctgcg gtcggccctg gccgccgggc
     1261 ttcccgaacc cgatgttacg gcctggcagg cgcagataaa cgaagcgatc gacgggctcg
     1321 ttccctggat ggcggaacgg tccagcgccg gctacgttcg ccgctgccat ggcgacctgc
     1381 acctgggaaa cctttgcctg tgggagggca agcccgtgcc gttcgacgcg ctggagttcg
     1441 acgaggcctt ggcgacgatc gacgtcggtt acgacctcgc cttcctgctg atggatctgg
     1501 atcaacgcgt cgggcgcgcg gcggcgaatc gcgtgatgaa ccgctacatt gcccgtaccg
     1561 gcgatgccag gataatggcc ggcctgccgg tcttcctgtc gcaacgggcg atcatccgag
     1621 cccatgttct tgcggcgatg ggtcaggacg gtgccgccta cctaacggcc gctcggcgct
     1681 atcttgcacc ggccgaacct gccgttattg cgattggcgg cttgcaaggc accggcaagt
     1741 ccaccctcgc ccgcgccttg gcgcctgaac tgggccggcc gcccggtgca ttgatcgtgc
     1801 gaagcgacga gatccgcaag cgcctgttcg gcttgcaccc acaacagcgc ctgcagcaag
     1861 agtcctatgg ccccgacgcg aacatcgcga cgaacagcgc gctgatcgag cagacacggg
     1921 tcgccgccga agctggccat accgcgatcg tcgatgcgac cttcctggac atcgagaccc
     1981 gtcgcgatct aaaggccgcg gtcggctcga ccggcgtgcg cttcctcggc atctggctgc
     2041 aagcgccgct tgccctgctt gagcaacgag tcgccgcgag gaccggtgac gcatcggatg
     2101 ccacggttgc cgtcttgcgg cgttcggccg gaaatgatcc cggcccggtg gattggctgg
     2161 cggtggatgc cagcgacgca acccgagcgc tagcctccgt ccgccaggca ttgctcaacg
     2221 ccctgacgtg atcaccctcg gcagcttcat gccaccgacg gcatccagcg ccgatgc
//
LOCUS       DASWQN010001229         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1968447, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001229 DASWQN010000000
VERSION     DASWQN010001229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968447"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(443..1519)
                     /locus_tag="VGC82_20855"
     CDS             complement(443..1519)
                     /locus_tag="VGC82_20855"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEY0425780.1"
                     /translation="MTRSYRIASVPADGIGPEVIAAGLEVLDAVASRDGGFKLIVDHY
                     DWGSNWYRKHGEFMPADGLARLRAADAIYFGAVGDPDIPDHLSLWGLRLQICQGFDQY
                     ANVRPTRILPGVTSPLRDVGIGDLDWVIVRENSEGEYAGQGGRTHRGLPEEVATETAI
                     FTRRGVERIMRFAFDIARSRPRKHLTVVTKSNAQRHGMVFWDEIAALVSLDYKDVTWD
                     KELVDAMTMRMVRKPKTIDTVVATNLHADILSDLAAALAGSLGIAPTGNIDPERRSPS
                     MFEPIHGSAFDITGKGIANPVATFWTGAMMLEHLGEKPAADLLMRAVEQVCAAGILSP
                     DLGGTATTQAVTKAVVEAVRGMNR"
     gene            complement(1598..>2812)
                     /locus_tag="VGC82_20860"
     CDS             complement(1598..>2812)
                     /locus_tag="VGC82_20860"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEY0425781.1"
                     /translation="PRNLRVEPMAEKLARIDDVGGPDTHSNYPLGWAMASNTPLRRYK
                     QNTHGGGIRDPLVISWPAGIRPHGELRHQFAHASDLAPTLLEVIGIDPPAAINGIPQM
                     PVEGSSFAPSLNDPSAPSKAKPQYFEMFGHRGIWDGGWKAVAYHPPGTPYETDKWELF
                     HLDQDFSETDDLADRQPEKLAALVRLWWEEAGKHKVLPLDDRFGPRFIENATRFQGAR
                     RNYVFHAGMGHVPTEVAPDVRSRGYSIEALVRMEAASEGVLIAHGDATSGYSLFLRNG
                     RLVHDMNIGGEHVVVESDRQVQEGDRRLGVRVSRLSRELGPTPITGPGLSAFTLLIDG
                     EAAGRIESRLGFYNFISWSGLDIGRDRGSPVSHYDAPFEFTGKLIKVVVTMDDDQVLD
                     GDGVGRARLSAE"
BASE COUNT          490 a          896 c          883 g          543 t
ORIGIN      
        1 gcaaatcaaa tttatttcat aatatttctt aatgcgacac agttaccgga tttgcggcaa
       61 tctgagcagt taccgtttag ttatggtcat gctcctgcgg ggatcgaagc gcggtctggg
      121 cagtgaccat cttatttatc ggatgctgta atggaatcag gcggcgatat aggcgaacgt
      181 cggcgatgaa accggatgcc ctggatgctc caccgcgata ccgccagtct tgacgaagcc
      241 tccccagtct tgacgaaagt cctcatgaag gcagcattat cgggcaacta agtgaagcgg
      301 actgtggcta ccaaccgggg catggtttaa cgtggtttcc gaccgttaag cggattgcta
      361 atcatcgacc tgacacaggt tttcgcaggc ccgtattgtt cctatctgct cggacttctg
      421 ggggcggagg tccgtcccgg ccctaccggt tcatcccacg caccgcctcg accacggctt
      481 tcgttaccgc ctgcgtcgtc gccgttccgc ccaggtcggg cgacaggata ccggcggcac
      541 agacctgctc caccgcccgc atcagcagat cggcggccgg tttctccccc aggtgctcca
      601 gcatcatcgc cccggtccag aacgtcgcta ccgggttggc gatccccttg ccggtgatgt
      661 cgaacgcgct gccatggatc ggctcgaaca tcgaagggct gcgccgttcg gggtcgatat
      721 ttccggtcgg agcgataccc agcgaaccgg ccagtgccgc cgcaaggtcc gacagaatgt
      781 ccgcatgcag attcgttgca accacagtat cgatggtctt tggcttgcgg accatccgca
      841 tggtcatcgc atcgaccagt tccttgtccc acgtcacgtc cttgtagtcc agcgacacca
      901 gcgccgcgat ctcgtcccag aacaccatgc cgtgccgctg cgcgtttgat ttggtcacca
      961 ccgtcaggtg cttcctggga cgcgaccgag cgatgtcgaa ggcgaagcgc atgatccgct
     1021 cgacgccgcg gcgggtgaag atcgcggtct cggttgcgac ttcctccggt aggccgcgat
     1081 gggtgcgccc gccctggccg gcgtactcgc cttcggagtt ctcgcgcacg atcacccagt
     1141 caaggtctcc gatgccgacg tcgcgcagcg gggaggtaac gccgggcagg atgcgggtcg
     1201 gacggacatt cgcgtactgg tcgaagccct ggcagatctg cagccgcaag ccccacagcg
     1261 agaggtggtc cgggatgtcg gggtcgccaa cagcgccgaa atagattgca tccgcggccc
     1321 gcagccgtgc cagcccatcg gccggcatga actcgccgtg cttgcggtac cagttcgatc
     1381 cccagtcata gtggtccacg atcagcttga acccaccatc gcgggaggcg accgcatcca
     1441 gcacttccag tcccgcggcg atgacctcgg gcccaatgcc gtcggcgggg acggaggcga
     1501 tgcggtagct acgggtcatg ggacggctcc tgatgcgctc atccccgttg gtagcgcttg
     1561 tcgctcgggg ttccaagatc ccggcgaatg cgggtcatca ctccgccgac agccgcgccc
     1621 ggccgacgcc atcgccatcg agcacctgat catcgtccat ggtgacgacg accttgatga
     1681 gcttgccggt gaattcgaac ggcgcgtcgt aatgggacac cggcgatccc cgatcgcgcc
     1741 cgatgtcgag gccggaccac gatatgaagt tgtagaagcc aagtctggac tcgatccttc
     1801 cggctgcctc gccgtctatc agcaaggtga aggcgctcag acccgggccg gtaatcggtg
     1861 tcggccccag ctcacgggaa agacggctta cgcggacccc gaggcggcgg tcgccttctt
     1921 gcacctggcg gtcggattca acgacgacgt gctcgccgcc gatgttcatg tcgtgcacca
     1981 ggcgcccgtt ccgcaggaaa aggctgtatc ccgacgtcgc gtcgccgtgg gcgatcaaca
     2041 cgccttcgga ggcggcctcc atccgcacaa gcgcctcgat cgaatagccg cggctgcgca
     2101 catcgggtgc cacttccgtg gggacatggc ccattccggc gtgaaacacg tagtttcgcc
     2161 gcgcaccctg gaagcgggtg gcgttttcga tgaagcgcgg gccgaagcga tcgtcgagcg
     2221 gcaggacctt gtgtttgccg gcctcctccc accacagccg caccagcgct gcgagctttt
     2281 ccggctgccg gtccgcgagg tcgtcggttt cggaaaaatc ctggtccaga tggaacaatt
     2341 cccacttgtc ggtctcgtac ggcgtgccgg gcggatggta agcaaccgct ttccagccgc
     2401 cgtcccaaat cccgcggtgt ccgaacatct cgaaatactg tggcttcgcc tttgaaggcg
     2461 cgctgggatc gttcaggctg ggggcgaagc tgctgccctc gaccggcatt tgcgggatgc
     2521 cgttgatggc cgcgggcggg tcgatgccga tgacctccag cagcgtgggt gcaaggtcgc
     2581 tggcatgggc aaactggtgg cgcagctcgc cgtggggccg gatgccggca ggccacgaga
     2641 tcaccagcgg gtcgcggatg ccgccgccat gagtgttttg cttgtagcgc cgcaacggcg
     2701 tgttcgaagc catcgcccag cccagcggat agttggaatg ggtgtcgggg ccaccgacgt
     2761 catcgatgcg tgccagtttc tcggccatcg gttcgacgcg aaggtttctt gg
//
LOCUS       DASWQN010001230         2517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1704517, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001230 DASWQN010000000
VERSION     DASWQN010001230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704517"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VGC82_20865"
     CDS             complement(<1..271)
                     /locus_tag="VGC82_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY0425782.1"
                     /translation="MTKRMLIDATHAEETRVVVLDGTRLEDFDVETSTKRQLKGNIYL
                     AKVVRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQIPVADRQALI"
     gene            902..1813
                     /locus_tag="VGC82_20870"
     CDS             902..1813
                     /locus_tag="VGC82_20870"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018979094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY0425783.1"
                     /translation="MSSAPAVTRRLLLRQFSAGMSIFGTFLLPPVLHDAMARAAVARD
                     TMTLKPGPTGKGGPHDRPFVARPVPLVMLDPGHGGKDPGAIGISGTYEKQVALAAALE
                     LKRQLEAGGRYRVELTRTKDVFIPLDGRVEYAQRRGASLFVSMHADALSDHTVRGASV
                     YTLAQTASDAQTAALARKENSADRFIGRQWQAASPEVSHILASLVRQETRVGSVRIAR
                     NLVGSLDQDLPMLQNPDRHAGFVVLKAADIPSVLVEMGFMSNPRDEAALRRADHRKLV
                     AQAMHRAVDGFFAGTGHVADSSAPNLG"
     gene            2023..>2517
                     /locus_tag="VGC82_20875"
     CDS             2023..>2517
                     /locus_tag="VGC82_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY0425784.1"
                     /translation="MRGLLGAVLGIVLLGSVVGAAGFYLAYEHFSADLPDVDGLRNYQ
                     PPVMSRIYAGDSRLLAELASERRIFVPITAIPDIVKQAFISAEDQNFYVHRGIDPLAI
                     ARAALFDLAHAGQGRRPIGASTITQQVAKNMLLTNQMSFARKAKEAILALRIEQNVSK
                     DRILE"
BASE COUNT          465 a          801 c          797 g          454 t
ORIGIN      
        1 cgatcagtgc ctgacggtcg gcgacgggga tctgatagta gtcgggatga atttcgctga
       61 acgccaggaa tccgtggcga ttgccgccgt attcgacgaa cgccgcctgt aggcttggct
      121 cgaccctgac taccttcgcg aggtaaatgt tgcctttgag ttgccgcttc gtcgatgtct
      181 cgacatcgaa atcttcaagt cgagtaccgt ccagcaccac gacgcgggtt tcttccgcgt
      241 gcgtggcgtc gatcagcatg cgcttcgtca tctagtcctg aactccggtt tgccgccggt
      301 atgcgcgagc cgggccgttg ggcctcggtc agcggtcggc atgcaaggct tgcgggaagg
      361 ggcccgcccg aaggttcagg aaaacgatgg cgatcggtgc acgcggcagc gctgataccc
      421 caaagatcgg gactacccga gaaacaggga ccacccgtaa taccggatga aagtccaaat
      481 tcggatagat gtcgcgcgac gtgtctcatc agtccctggc gttcggatgg cgcgtcgcga
      541 aacgctgccc ccgtgtgaaa gtctgcgctg gcgctgcccg cgtggcttga tccgcggcgg
      601 acaggacccc ggcgcgccac aagtccacat ggcgggcgcg acacggacgg cgacgtggcc
      661 acggcgaggc ggatccttcc gcacacttgc aagtctgggt cggaccagac cagccaaggg
      721 atcacgcgcc gccggaaaag gccaagggac cgcgatacgt cgccaccccc gttcgtcgcc
      781 ggagcaaccc gaccatgaag caaaccgtcc gctaccgcaa gcggctcatt tataaacgcc
      841 ctgaactcgc cgtaatcaac atacaagctg cctgaacgta tccaatctgt taagagcaac
      901 catgagttcc gcccccgccg tcacgcgtcg cctgctgctg cgccagttct cggcgggcat
      961 gtcgatcttc ggtactttcc tgttgccgcc ggttctgcat gatgccatgg cgcgtgctgc
     1021 ggtggcgcgc gatacgatga ccctgaagcc aggtccgacc gggaagggcg ggccgcacga
     1081 ccggcctttt gtcgcccggc cggtgccgct ggtcatgctg gaccccggtc acggcggcaa
     1141 ggaccccggc gccatcggca tatccggcac ttacgagaaa caggtagcac ttgccgccgc
     1201 gctggaactg aaacgtcagc tagaagccgg cgggcgctac cgggtggaac tgacccggac
     1261 gaaggacgta ttcatcccgc tggacggccg ggtcgaatat gcgcagcgcc gcggagcctc
     1321 gttgttcgtg tccatgcacg cggatgcgct gtccgaccat acggtacgcg gcgcctcggt
     1381 ctataccttg gcgcaaacgg cctcggatgc gcagaccgcc gcgctggccc ggaaggaaaa
     1441 cagcgccgac cgcttcatcg gccggcagtg gcaggcggcc tcgcccgaag tatcgcacat
     1501 tctcgccagc ctggtgcggc aggaaacccg ggtcggttcc gtccgtatcg cccgcaacct
     1561 tgtcggaagc ctggatcagg acctgccgat gttgcagaat cccgatcggc acgccgggtt
     1621 tgtcgtgctg aaggcggccg acatccccag tgtgctggtt gaaatggggt tcatgtccaa
     1681 cccgcgggac gaggctgcct tgcgccgcgc cgatcaccgc aagctggtcg cgcaggcgat
     1741 gcaccgagcc gttgacggat ttttcgccgg cactgggcac gtggcggaca gttcggcacc
     1801 aaatcttggt tgagcgccgc gtttagctgt cggaaatagc aatctcgttg ctggcgatct
     1861 tacgcaaaag cgttcatatc gccggtcatg acaggaccac tgaccgctgg cgacaagctg
     1921 gcgccggatg cccgcgatcc acgcggcaag ccggaaccga aacgcggcca aaagctgccg
     1981 ggaaaagccc ccgttcggcc gattggccgg ttaaagcggg cgatgcgcgg cttgctggga
     2041 gcagtgctgg ggatcgtgct gctcggcagt gtcgtcggag ccgccggctt ctatctggcc
     2101 tacgagcact tcagtgccga tctaccggat gtcgatggct tgcggaacta ccagccgcct
     2161 gtcatgagcc ggatttacgc tggcgattcg cggttgctgg cggagctggc gtccgaacgc
     2221 cggatatttg tgccgatcac cgcgatcccc gatatcgtca agcaggcctt catttctgcg
     2281 gaagatcaga atttctacgt ccatcgcggc atcgacccgc tggcgatcgc ccgcgccgca
     2341 ttgttcgacc tcgctcatgc cgggcagggg cgccgtccga tcggtgcctc gaccattacg
     2401 cagcaagtcg ccaagaacat gctgctgacc aaccagatgt cgttcgcccg caaggcaaag
     2461 gaggccattc tggcgcttcg gatcgagcag aacgtcagca aggatcgcat cctcgag
//
LOCUS       DASWQN010001231         5851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2629495, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001231 DASWQN010000000
VERSION     DASWQN010001231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2629495"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VGC82_20880"
     CDS             <1..729
                     /locus_tag="VGC82_20880"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEY0425785.1"
                     /translation="NLDGTDVTSQLTGGQIGANIALRDTTLPTYQAELDEFSQGLSSR
                     FAAQGLTLFTDTSGAVPAGGGTQVQAGYVGYAGTIQVNSAVAADPSLLRDGTNNATSG
                     GFTPNPPGGPAGLTALIANIVNYTFSASAQAGAAQPPLNTSGLGASGNLAAPFNAAHV
                     ALSGFATSLVSSQAQQSATATSHLAAEEALQTSLNAKASAVSGVNMDTEMSHMLSLQS
                     AYSANARVIAAVQSMFAQLLQAIQ"
     gene            726..1814
                     /locus_tag="VGC82_20885"
     CDS             726..1814
                     /locus_tag="VGC82_20885"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY0425786.1"
                     /translation="MTGGISPTGVGYSHAATLTANASKIHQQLSVLTEQASTGLIADT
                     YAGLGNGAAVSLDLNPQLTALQTYKSNIDRASGSMQVTQAAMTQLQQIAAKFVADIPN
                     LNGINSSEVDSVAANARAALSQVADLLNTKNGNVYVFGGQDSANPPVPGSDAILSSGF
                     YSQINAAVSGLAANGATATTAATLTIAGSNGSGTSPFSAYLSQPAASLSPPVVQTGDT
                     TMVATGLLASGNSTALSTGPSTTGSYMRDLMRALATLGSMSSTQLNDPGFNALVSDTG
                     ASLNSAVTAMAVDVGVLGTTQANLTKTQTQLSDTTTALTGQLTNVQDADMAATLSKLT
                     AMQTQLQESYRLITSANSLSLINFLPAA"
     gene            1945..2757
                     /locus_tag="VGC82_20890"
     CDS             1945..2757
                     /locus_tag="VGC82_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY0425787.1"
                     /translation="MGAMVALQSLQSTQQQLSVAQKQVSTGFRVADSSDDGAAYAIAQ
                     GIRANVGALTSANQQLGNVQGLLSVTQSGLNNISNTMMSMRDLLVKLSDSSVQGNDRS
                     NYEANYKEILKNVHRDLEDANYNGKTLIGDLGNATGFGSVNVARNETGATYGIGTFSG
                     SALYGSISGTLGTSTAVQALLTSSGTFISMQNKVNNQLNKVGSSINYISNQTTYNNDK
                     INALNGGLGSLIDADLAKESAQLTALQIRQQLGTQALSMANQAPQSLLSLFK"
     gene            2963..3427
                     /locus_tag="VGC82_20895"
     CDS             2963..3427
                     /locus_tag="VGC82_20895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HEY0425788.1"
                     /translation="MSNLVLELRQGEVMIVNGAPIRFRTRSRIELTAKARFLFGKQIM
                     PPDQVDSPARRIYFALQSAYIGNESEREAGLQTARHFIADFQQATTSELVRTILTQSL
                     IAAEEDDCYQALKLVRRIIQHEDAVLGKTPNVSVPTGNSDRHAEREPCHPGL"
     gene            3393..3740
                     /locus_tag="VGC82_20900"
     CDS             3393..3740
                     /locus_tag="VGC82_20900"
                     /inference="COORDINATES: protein motif:HMM:NF018959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HEY0425789.1"
                     /translation="MLNANHAIQAYKTASRYRSQRDQEADVFRQAIGGLKSAAGASPI
                     QQIRALADNRRLWMMVIDLMRDPANALPEPLRASIVSVGLTVHREMDKEAPDFDFLIS
                     INENIAAGLAGKS"
     gene            3745..4593
                     /locus_tag="VGC82_20905"
     CDS             3745..4593
                     /locus_tag="VGC82_20905"
                     /inference="COORDINATES: protein motif:HMM:NF018458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1217 domain-containing protein"
                     /protein_id="HEY0425790.1"
                     /translation="MSGSLSYLASFGGLSSDGGLSLLNALYGIGGSSAGSSGQTAVQA
                     LASAERTQTQQVKSTAAQPEVLHAITAFTKGVNSAKSVAQLLANPAVMKVLLTANGLG
                     DQAVYTALATKVLTSNLNDPKSLANTLTDPRWKTLAKTYNFAADGLSGIQNPKNIATV
                     ANGYAQVTWQNNQDAVTPGLTNALKFKAAASTITSVDQILGDLTIRTVVTGALGIPPQ
                     IAFQSMDAQEKAISSRLDITKFKDPKYVETFVQRYLVANSSDASSSSSTPDLTTLAIQ
                     GQGLFV"
     gene            4627..>5851
                     /locus_tag="VGC82_20910"
     CDS             4627..>5851
                     /locus_tag="VGC82_20910"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0425791.1"
                     /translation="MDTIHVTAGADALRDRAAQLIEAGRIGAARPLLAAARALAPPSA
                     ELALVCAQLAMATGAWEKAIEELDSGIAAAPSHAGLRKCRADARQRIGDHEGAARDAA
                     EAVCIHSGDPQAKAILGAAMLGLGRTVDAVACLAEAVAAAPREVGYNEALASALERAG
                     DTDAALRVLADAILACPGAVSVRNAAILLCIRRRDFSQAVTVAERACSVGVADACTFG
                     MKGHALSSLGEHDRAMIAYQDALKLGPEDPYVRHLVVSSGAMPDSRRAPEDYIRTVFD
                     GYADHFESHLISLRYVIPAMIRSLLQGHPRIAAGLPLGPVLDLGCGTGLVALVIADLP
                     VGPITGVDLSPRMLAHAQAKEIYTELRKADIIADLIAHDQRWPLILAADVLCYFGSLE
                     ELLDLVHRRLEPGGWF"
BASE COUNT         1228 a         1833 c         1686 g         1104 t
ORIGIN      
        1 aacctggacg ggacggatgt caccagccaa ctgaccggcg gccagatcgg cgccaatatc
       61 gcgctgcgag acactaccct gccgacttat caagccgaac tggatgagtt cagtcagggc
      121 ctgtccagcc ggttcgccgc ccagggactg acgctgttca cggataccag cggtgccgtt
      181 ccggccggcg gcggaaccca ggttcaggcc ggctacgtcg gctacgctgg cacgatccag
      241 gtcaattccg cggttgctgc cgatccgtcc ctgttgcgtg acggcaccaa caatgccacc
      301 tcaggtggct tcacacccaa tccgccgggc ggtccggccg gtttgaccgc gctgatcgcg
      361 aacatcgtca actacacctt cagcgccagc gcgcaggccg gcgctgccca gccgccgctt
      421 aacaccagtg gcctcggcgc gtccggcaat cttgctgcac cgttcaacgc ggcccatgtc
      481 gcgctgtcgg gtttcgcgac cagcctggtg tcgtcgcagg cacaacaaag cgccacggca
      541 accagccatc tggcagccga ggaggcgctg cagaccagcc tcaacgccaa ggcgtcagcc
      601 gtcagcggcg tgaacatgga taccgaaatg tcgcacatgc tcagcctgca aagcgcctat
      661 agcgcgaacg cccgagtgat cgccgcggta cagtcgatgt tcgcccaatt gctccaggcg
      721 atccaatgac cggcggtatc agcccgaccg gcgtgggtta cagccacgct gccacgctga
      781 ccgccaacgc cagcaaaatt catcagcaac tcagcgtgct gaccgaacag gcaagcaccg
      841 ggctgatcgc cgacacctac gccgggcttg ggaatggtgc cgccgtgtcg ctggacctga
      901 acccgcaact aaccgcgctg cagacctaca agagcaacat cgaccgggcg agcgggagca
      961 tgcaggtcac ccaggccgcg atgacccagc tccagcagat agcggccaag ttcgttgcgg
     1021 acatccccaa cttgaacggc attaattcgt ccgaagttga cagcgtcgcc gccaatgccc
     1081 gggccgcact ctcgcaggtc gccgatttgt tgaacacgaa gaatgggaac gtctatgtat
     1141 tcggtggcca ggacagcgcc aacccgccgg tgcccgggtc cgacgcgatc ctgtcgtccg
     1201 gcttctatag ccaaatcaac gccgccgttt ccggcctggc ggccaacgga gcgaccgcga
     1261 cgaccgctgc gacactgacc atagccggat cgaacggttc gggcacctcg ccgttctcgg
     1321 cctacctgtc acagccggcc gcctcgctca gtcctccggt ggtgcagaca ggcgatacca
     1381 ccatggtagc gaccggcttg ctggccagcg ggaactccac ggcgctgtcc accggtccat
     1441 ccaccacggg atcctatatg cgcgacctga tgcgcgccct ggccaccctt gggtcgatgt
     1501 ccagcaccca gctaaacgac cccggcttca acgcgttggt cagcgatacc ggagccagcc
     1561 tgaacagtgc cgtcaccgcc atggccgtcg atgttggcgt gttgggaacc acgcaggcca
     1621 acctgaccaa gacgcagaca cagttgtcgg acaccacgac ggcgctgact ggccagctaa
     1681 caaacgttca ggatgccgac atggccgcga ctttgtccaa actgaccgcc atgcaaacac
     1741 aacttcaaga atcctatcga ctgattacca gcgccaacag cctgtcgctg ataaactttc
     1801 ttccggcagc gtaaaaaagc ctaatggatt catgatccgt taactttccg agtgcattgt
     1861 ctcttcgagc ccgcaaaaag cgagcatttt catctggaca caaagggtta agacaccatg
     1921 tccctaaact cagtaaacac caacatgggc gcaatggttg cgctccagtc cttgcagagc
     1981 acgcagcagc aactgtcggt cgcgcagaag caggtgtcca ccggatttcg cgtcgccgac
     2041 tcgtctgatg acggcgccgc atacgcgatc gcgcaaggca ttcgtgccaa cgtcggcgcc
     2101 ctgaccagcg ccaaccagca gcttggcaac gtgcagggtt tgctttcggt gacccagtcg
     2161 ggcctgaaca acatctccaa caccatgatg agcatgcgcg atttgctggt caaactgtcg
     2221 gatagcagcg ttcagggtaa cgaccgctca aactacgaag ctaactacaa agaaattctc
     2281 aagaacgtgc atcgggatct tgaggatgcc aactacaacg gcaaaacact gattggcgat
     2341 ctgggcaatg ctaccggctt cggctccgtc aacgtggcac gcaatgaaac cggtgcgacg
     2401 tatgggatcg ggacgttcag cggctccgct ctttacgggt cgatcagcgg gacgctggga
     2461 acctccaccg cggttcaggc gcttctgacc tcgagcggca cgttcatcag catgcagaac
     2521 aaggtgaata accagctcaa caaagtcggc agctcgataa actatatcag caatcagacc
     2581 acgtacaaca acgacaagat aaatgccctg aacggtggcc ttggatcgtt gatcgacgct
     2641 gatttggcga aagagtcggc tcagctcacg gcgttgcaga ttcgtcagca gctcggtacc
     2701 caggctctct cgatggcgaa ccaagccccg cagtcgctgc tcagcctctt caagtaaacg
     2761 gcttttcggc ggcgtgaccg ggctgatttc agcgcggtcg agccgccggc cgatactttg
     2821 gaagccgagc aagacttcga tctggagcgt gctcgtctgt tataatgacc tgcggcgaag
     2881 gttgcgttcg aacacaagcg gaagcacgac ccgccttgat aatcttactc aggcttctcg
     2941 cttgcgccgc gaaagggacc gtatgtctaa tcttgttctg gagttgcgcc aaggcgaagt
     3001 catgatcgtg aacggagctc ctatcaggtt ccgtacgaga tcgcgcatcg agctaacggc
     3061 caaagcccgc ttcctgtttg gcaagcagat catgcccccg gaccaggtgg acagtcccgc
     3121 ccgacggatc tacttcgcac tgcaatccgc ctacatcggc aacgaatcgg aacgcgaggc
     3181 cgggctgcaa accgcccgtc atttcatcgc cgactttcag caggcaacga cctctgagct
     3241 ggtccgaacg attctaactc agtcgctgat cgcggcggag gaagacgact gttatcaggc
     3301 tttgaagctc gtccgccgga tcatacagca cgaagatgcc gtgcttggaa aaacaccgaa
     3361 cgtctccgtt ccaacaggga atagcgatcg ccatgctgaa cgcgaaccat gccatccagg
     3421 cctataaaac cgcctctcgc taccgctcgc agcgcgatca ggaagcggat gtcttccggc
     3481 aggccatcgg cggactgaaa agtgccgctg gtgccagccc aatccagcag atccgcgcgt
     3541 tggccgataa tcgtcgcctg tggatgatgg ttatcgacct gatgcgcgac ccagccaatg
     3601 ccctgcccga gccgttgcgc gcttcgatcg tttcggtcgg gctgacggtg caccgggaaa
     3661 tggacaagga agcgccggac ttcgatttcc tgatatcgat caacgaaaac attgctgccg
     3721 gcttggccgg gaaatcctga tcccatgagc gggtcgctca gctacctggc atcgtttggc
     3781 ggcttgtctt ccgacggggg actgtcgctt ctcaatgcat tatacgggat tggcggctcg
     3841 tcggccggtt cttccgggca aaccgccgtg caagcattgg catcggcgga gcgaacccag
     3901 acgcaacagg taaaatcgac ggccgcgcag ccagaagtcc tgcacgcgat caccgccttc
     3961 accaaggggg tgaattcggc caaaagcgtt gcgcaacttc tcgctaatcc ggcggtaatg
     4021 aaggtgttgc tgaccgccaa tggtctgggc gaccaggccg tatacaccgc tctggcgacg
     4081 aaggtcctga cgtcgaacct gaacgaccca aaatcacttg ccaatacgtt gaccgatccg
     4141 cgctggaaga cactcgcgaa aacctacaac ttcgcggctg acggattgtc aggaattcaa
     4201 aatcccaaga atatcgcaac cgtcgccaac gggtatgcac aggtcacctg gcaaaataat
     4261 caggatgcgg tgacgcccgg gcttactaac gctttgaagt tcaaggcggc ggcatcgact
     4321 atcacttccg tcgatcagat tctcggcgac ctgacaatac gcaccgttgt caccggcgca
     4381 cttggtatcc cgccgcagat cgctttccaa tcgatggacg cccaagaaaa ggcgatcagc
     4441 agccgcttgg acatcacgaa gttcaaggac ccaaagtatg tcgagacgtt cgtgcaacgc
     4501 tatctggtcg cgaatagctc cgacgcttcg tcttcttcgt caacgcccga cctgacaacc
     4561 ctggcgatac agggacaggg cctgtttgta tgacggcgtt tttcacggct caaaaggagc
     4621 cccctcatgg acacgattca cgtcactgct ggtgctgatg cgcttcgcga tcgagcggct
     4681 cagcttatcg aggccggccg aataggcgcc gccaggcctc ttctggcggc agcgcgggca
     4741 ttagcaccgc catcggccga actcgcattg gtttgcgctc aactggccat ggctactggc
     4801 gcatgggaaa aggcgataga ggaactcgat tccggtatcg ccgcggcgcc ttcccatgcc
     4861 ggtttacgca agtgccgggc tgatgcccgg caaagaattg gtgaccatga aggtgccgcg
     4921 cgcgatgcgg ccgaggccgt ctgcatccat tccggcgatc cgcaagccaa ggcgattttg
     4981 ggtgcggcga tgcttggcct tggacgcaca gtcgatgcgg tagcctgtct ggctgaagcg
     5041 gtggccgccg cgccgcgtga agtgggttat aatgaggccc tggcttccgc gcttgaaagg
     5101 gcaggtgaca ctgacgctgc gctacgggtt ctggccgatg ccattctggc gtgtccgggc
     5161 gccgtgagcg tacgcaacgc cgctatcctg ctttgcattc ggcggcggga ttttagccag
     5221 gcggtgacgg tcgccgaaag agcctgttcc gtcggggtgg ccgatgcctg caccttcgga
     5281 atgaaaggtc atgcgctttc cagcctgggc gagcacgacc gagccatgat cgcctaccag
     5341 gacgcgctga agctgggtcc ggaggaccct tatgtgcgcc acctcgtcgt atcctcgggt
     5401 gcgatgcccg attcgaggcg tgctcccgaa gattacatcc gcactgtctt cgacggttac
     5461 gccgaccact tcgaaagcca tttgatttca ctgcgatacg tcattcctgc catgatccgt
     5521 agtttgcttc agggccatcc aaggatcgcg gccggcttgc cgcttgggcc ggtgctcgat
     5581 cttggatgtg gaaccgggct ggtcgccctt gtgatcgccg atctaccggt gggaccgatc
     5641 accggcgtcg atctgtcgcc gcggatgctg gcgcacgcgc aggccaagga aatctacacc
     5701 gaactccgga aagccgacat cattgccgac ttgatcgccc atgaccagcg ttggccactg
     5761 atcctggcag cggatgtgct gtgttatttt ggttcgctgg aggaactgct cgatctcgtt
     5821 catcggcgtc tggaaccggg cggctggttc g
//
LOCUS       DASWQN010001232         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1309674, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001232 DASWQN010000000
VERSION     DASWQN010001232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309674"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..826)
                     /locus_tag="VGC82_20915"
     CDS             complement(242..826)
                     /locus_tag="VGC82_20915"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEY0425792.1"
                     /translation="MHRFALRNILALFVWCWLAADAAAATLAGVTLPDSYTVDGQTLV
                     LNGIGLRTLTIFNVKVYVAGLYLPRQSHDSRQILNSPGPKVVLLEFIRSGSKAQVEKQ
                     YRAGEETNCGDGGCDPADQADFERLVAAAPAVNPGDTSTYIFTAKGVRVFANDRLIGD
                     FANRDLAYHLLAGFIGDHPPSQRLRRQLLGQPED"
     gene            1091..1345
                     /locus_tag="VGC82_20920"
     CDS             1091..1345
                     /locus_tag="VGC82_20920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425793.1"
                     /translation="MDVTIVEDQGTHLLISQDNRFAIVERRNNRLYNCHGGKRDGIPL
                     ENLSAVGTILDSTDWGDETTARAAFNDIIARGTHLGETML"
     gene            1387..>2908
                     /locus_tag="VGC82_20925"
     CDS             1387..>2908
                     /locus_tag="VGC82_20925"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0425794.1"
                     /translation="METQKKAPGAIEQHPELPAPDAMLSVWSSWMEAATSSAKHWGGR
                     SGDGGTQWWQAAPDAAAEHALASGVKQFNELLAKDPVLHAIDQMWNANPLRDVVPVDW
                     AEIARALRTVWMRSMRQPGKAISSAMEFNTRVLSAATEVWNDAARRWLSGTGGDAKPT
                     GPFDKRFAAPEWQGNPAYRTLKEIYLLASSWLLDQADADDMEEAERQRLNFHLRQFVD
                     AMSPSLLLLSNPEALRRAIETGGASLADGVRNLLHDLKEGRLSMVDATAFAPGRNLAM
                     TPGKVVHRNKLIELIQYTPTTDTVHKVPLLILPPWINKYYILDMQPKNSMVRFLVEQG
                     FTVFVVSWKNPDSSMDGTTIEDYMDLGPLEASDVIREITGSPTLNVMGYCIGGTLLAM
                     TLAWLAAKRDKRFGSATFMVSLQDFSKVGDTAVFLGEPAVDFVEQQMMERGYLDSREM
                     SNMFNLLRSNDLIWSNVVNNYLLGQKPPAFDLLYWNSDGTRMTRVAHSWYLRNTYVE"
BASE COUNT          581 a          908 c          918 g          501 t
ORIGIN      
        1 gatcaggatg tcatgcgcgc caacgccctg aggcgtcacc accgtgtcgc cacggatcac
       61 cagatcgacc atgtcttccc ccgcttgtcg tgttccctgc ggcacatcgt cgcccagccg
      121 aggcggatgg gcaaggcagg gcggacacgt gggccacggc actgccgact gtcttgttga
      181 ttcgcgcgca gccgctacga taatcgtggc cgccgtcagt ccggccctga cgccgccggt
      241 atcagtcctc cggttggccc agcagttgac ggcgcaggcg ctgggatggc gggtggtcgc
      301 cgatgaaccc ggcaagaagg tggtatgcca ggtcgcggtt cgcgaagtcg ccgatcagtc
      361 ggtcgttggc gaacacccgg acgcccttgg ctgtgaagat ataggtcgag gtatcgccgg
      421 ggttgaccgc gggggcagcc gccaccaaac gttcgaaatc cgcctggtcg gctggatcgc
      481 agccgccatc gccgcagttc gtttcctcgc cggcgcggta ctgtttttcc acctgcgcct
      541 tggatccgga gcggataaac tccagcagca ccaccttcgg accaggggaa ttcaaaatct
      601 gccgcgagtc atggctttgc cgcggcagat acaggccggc gacatagacc ttgacgttga
      661 aaatcgtcag cgtgcgcagc ccgatcccgt tcagcaccag cgtctgccca tcgacggtat
      721 agctgtccgg caaggtcacg ccggccaagg tggctgccgc cgcgtcagcc gccaaccagc
      781 accaaacgaa cagcgccagt atgttccgca aagcgaacct atgcaatttc tgtccgatcc
      841 tgaattatag gttccatcgc atgacctccc tttggcgcat gacctccctt tggcgcgcca
      901 acccaacgcc tctggggcaa cgagcgttcc tgaacttcat ggtcgcattg tggcgccgat
      961 caaagcgaac tgagcctgtg ccggtgcgtt accaaggaat gcccgcgtcg ccgggtcagt
     1021 gtcgaacgcc cccaaggggg cctggacgaa ggacacgtcg ggacgcgact gcttggcaag
     1081 ggaggcatgg atggacgtca ccatcgtcga agatcagggt acacacctgc tgatcagcca
     1141 ggataaccgc tttgccatcg tcgagcgacg gaacaaccgg ctttataact gccacggtgg
     1201 caagcgggac ggcattccgc tggagaactt gtcagccgtg ggcacaatcc tggacagcac
     1261 cgactggggc gacgaaacca ccgcccgagc ggcatttaac gacatcatag cgcgtggtac
     1321 gcacctcggc gaaacgatgc tttgacgccg tcggaagtta gagccgaatt ggaaggaaac
     1381 gctggcatgg aaacccaaaa gaaggcacca ggcgccatcg aacaacaccc ggaactcccc
     1441 gcgcccgacg cgatgctgag cgtgtggtcg tcgtggatgg aagctgccac cagctccgcc
     1501 aagcactggg gcggccggtc gggtgacggc ggcacgcagt ggtggcaggc cgcaccggat
     1561 gccgcggccg aacatgcgct tgcctccggc gtcaagcagt tcaacgagct tctggccaaa
     1621 gatccggtct tgcacgccat cgaccagatg tggaacgcca acccgctacg cgacgtggtg
     1681 ccggtggact gggcggagat cgcccgggcg cttcgtacgg tgtggatgcg ctcgatgcgc
     1741 caacccggca aggccatctc gtcggcgatg gaattcaata cgcgcgtgct cagcgccgcg
     1801 accgaggtgt ggaacgatgc cgcgcggcga tggctgagcg gcaccggcgg ggacgcgaag
     1861 ccaaccggac cgttcgacaa gcgcttcgcc gcgccggaat ggcagggcaa tccggcgtat
     1921 cgcacgctga aggagatcta tctgctcgcc tcgagctggt tgctcgatca ggccgacgcc
     1981 gatgacatgg aggaagcgga acgccagcgc ctgaacttcc acctccgcca gttcgtcgat
     2041 gccatgagcc catcgttgct gctgttgtcc aatccggaag ccttgcgccg agcgatcgag
     2101 accggcggag caagcttggc cgacggcgtg cggaacctgc tgcacgacct caaggaaggc
     2161 cggctgagca tggtcgatgc cacggctttc gcgcctggac gaaatctcgc catgacaccg
     2221 gggaaggtgg tgcaccgcaa caagctgatc gaactgatcc agtacacgcc cacgaccgac
     2281 acggtgcaca aggtgccgtt gctgatcctg ccgccatgga tcaacaaata ctacatcctc
     2341 gacatgcagc cgaagaacag catggtgcgc ttcctggtgg agcagggctt caccgtgttt
     2401 gtggtgtcct ggaagaaccc ggacagctcg atggatggca ccacgatcga ggattatatg
     2461 gacctcggtc cgctggaggc gagcgacgtc atacgcgaga tcaccggcag tccgaccttg
     2521 aatgtgatgg gttactgcat cggtggcacg ttgctcgcga tgaccctggc gtggctggcg
     2581 gccaagcggg acaagcgctt cgggtcggcg acattcatgg tgtcgttgca ggacttctcg
     2641 aaggtcggcg acaccgcggt attcctgggc gagccggccg tcgacttcgt cgagcagcag
     2701 atgatggaac gcggctacct ggacagccgt gagatgtcca acatgttcaa cctgctgcgc
     2761 tcgaacgatc tgatctggtc gaacgtcgtg aacaactacc ttctggggca gaagcccccc
     2821 gccttcgact tgctctactg gaacagcgac ggaacacgca tgacccgtgt cgcgcacagc
     2881 tggtacctgc gcaacactta cgtcgaga
//
LOCUS       DASWQN010001233         3551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_516875, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001233 DASWQN010000000
VERSION     DASWQN010001233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516875"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VGC82_20930"
     CDS             <1..382
                     /locus_tag="VGC82_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HEY0425795.1"
                     /translation="LLEQTAENMESLQRVLTRDEENRGQVNQAVLTLTERLGSLADTM
                     RANQQLMLRIAETQTALGPALHRLSELQGGDEASRGHLRSIENYMQRLVAENEQGRAR
                     ATADLRNDLRLLTRTVAALAEPQR"
     gene            388..1692
                     /locus_tag="VGC82_20935"
     CDS             388..1692
                     /locus_tag="VGC82_20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HEY0425796.1"
                     /translation="MAARTRRGHGGNGLDAWPGYVDALSTLLMVIIFVLLVFVLAQAF
                     LTVTLSGRSHELDKANQQLAALADALSLERGKSAELQLSVSQLGKELSARASERDRLA
                     AQLADTNRILQAANVANSQLQTQLTEASGQATATKAELADAQTQLAEMKRQIAELDRT
                     VAVDRDTLQAKLSELATLADQSRALAALRDELETQAQNAAARAMTEQQRREAVQAQLA
                     EEKKLGDSARAQIALLNQQIDQFKSQLASIAASLELTRRLNQDKDTQIANLGQKLNLA
                     LAARVEELQQYRSEFFGKLRSLLANRSGITVVGDRFVFQSEVLFPVGSAELTQAGIAQ
                     MTTLAVTIKDIASEIPSDLHWVLRVDGHTDPQPVKGGQFASNWELSAQRAITVVKLLI
                     ADGVPADHLAATAFGEHQPFGPGDTPEAYSKDRRIELRLTDR"
     gene            complement(2022..3194)
                     /locus_tag="VGC82_20940"
     CDS             complement(2022..3194)
                     /locus_tag="VGC82_20940"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY0425797.1"
                     /translation="MTINGRVVFSQMDEVRFGVPAAEAVAEQATRFGAERVFLMVSGT
                     LNRTTPEIAHVRGALGNRCAGLFDRMPAHSPRHAVIAAAEQARAVGADLIVTVGGGSI
                     TDAAKVVQLCLANDIRSAEALDALRPVKGPNGAMVAPDCNPPVVRQVSVPTTLSAGEF
                     SALAGVTDERTRVKELFRHPRIIPAAVILDPAITVHTPEWLFLSTGIRAVDHCVEGVC
                     SKEANPYADAQGLRGLTLLTSGLARVKADPSDLQARLDCQMGSWLSMGPLASGVPMGA
                     SHGIGYVLGAVFDIPHGHTSCIMLPFVMKWNKMANAERQAMVAAAMGHPERDAGDLLD
                     GFIAGLGMPRSLGAVNIGTDAFDRIAEGAMATPWIPRNPRSIDNPRQVREILELAA"
BASE COUNT          683 a         1142 c         1142 g          584 t
ORIGIN      
        1 gctgctggag cagaccgcgg agaatatgga aagcctgcag cgtgtcctga cgcgggacga
       61 ggaaaaccgc ggccaggtta atcaggctgt gctgaccctg accgaacggc tgggcagctt
      121 ggccgacacc atgcgcgcca accagcaatt gatgctgcgg atcgccgaaa cccaaaccgc
      181 gctgggaccg gcgttgcatc gcctcagcga gttgcaaggg ggcgatgagg ccagccgcgg
      241 gcacctgcgc agcatcgaaa actacatgca gcggctggtg gctgaaaacg aacaaggccg
      301 cgcccgtgcc accgccgacc tgcgcaacga cctgcggctg ctgacccgca cggttgcggc
      361 gctggctgag ccgcagcggt agggggcgtg gcagcgcgca ctcgcagggg ccacggcggc
      421 aacggactgg atgcctggcc gggctacgtg gatgcgctgt ccacgctgct gatggtcatc
      481 atcttcgtgc tgctggtgtt cgttctggcg caggcatttc tgaccgtgac gctgtccggc
      541 cgcagccatg aactggacaa ggccaatcag caactggccg cgttggccga cgccctgtcg
      601 ttggagcgag ggaagtcggc ggaactgcaa ctttccgtat ctcagcttgg gaaggaactg
      661 tctgcccgcg cttccgaacg cgaccgcctg gccgcgcaac tggccgatac gaaccgtatt
      721 ctgcaggcgg ccaacgtggc taattcgcag ttgcagacgc aattgacgga ggcttccggc
      781 caggcaacgg ccaccaaggc ggaactggcg gatgcccaga cccagctcgc cgagatgaag
      841 cggcagatcg ccgaactgga ccgcactgtc gccgtcgatc gggacactct gcaggccaaa
      901 ctttcggagc tggcaacgct ggcggaccag tcgcgtgccc tggcggcctt gcgcgatgag
      961 ctggaaacgc aggcgcaaaa tgcggcggca cgagccatga ccgagcagca gcgacgggaa
     1021 gcggttcagg cgcagctcgc ggaggagaag aagctggggg acagcgctcg ggcgcagatc
     1081 gcattgctga accagcagat cgaccagttc aaaagccagc tcgcctcgat cgccgccagc
     1141 ctggagctga cgcgcagact gaaccaggac aaggatacgc agatcgccaa tctggggcag
     1201 aagctgaacc tggcactggc ggcgagggtg gaggaactgc agcaatatcg cagcgagttc
     1261 tttggcaaat tgcgcagttt gctggcgaac cgatccggca tcaccgtggt cggcgaccgg
     1321 ttcgtgttcc aaagcgaggt gctgtttccg gtcggcagcg cggaactgac tcaggccggg
     1381 atcgcgcaaa tgacgacctt ggcggtgacg atcaaggata tcgcttcgga aatcccgtcc
     1441 gatctgcact gggtcctgcg cgtggacggc cacaccgacc cacagccggt gaagggcgga
     1501 caatttgcct cgaactggga gctttcggcg caacgcgcga tcaccgtagt gaaactgctg
     1561 atcgcggatg gcgtgccggc ggatcatctt gccgcgacgg cattcggcga acaccagcca
     1621 ttcggacccg gcgatacgcc cgaggcgtat tcaaaagacc gacggatcga gctgcggctg
     1681 acagaccggt aggccggccc aggtcgatga gccggtcagg tcgatgagag agcccaaaca
     1741 gacggaaggc cccggaataa atcccgaagc cttcccattg tttgtaagcg tccgctgccg
     1801 gaccaaaaaa tcctacgccg acgcggttac gccggcgatc caagtcttgg cgacggaagc
     1861 cacggaacaa tgagacactg gatcacctcc tttcagttgt tgctacgacg acggtagtgt
     1921 gcatccgatt cgcgcagcgg gcaagaggtt ccgcgcagag gtccgcgcaa tcgggcgcaa
     1981 cggcatcggc cgatcgccgg catcctgttt gtcatcggat cctacgctgc aagttcgagg
     2041 atctcgcgga cctgccttgg attgtcgatt gaacgcgggt tgcgtggaat ccagggcgtc
     2101 gccatggcac cttccgcaat gcgatcgaac gcatccgtcc cgatgtttac cgcgcccaag
     2161 ctgcgcggca tccccaggcc ggcgataaac ccgtccagca agtccccggc atcccgctcc
     2221 ggatgcccca ttgcggcagc caccatcgcc tgccgctccg cattcgccat cttgttccat
     2281 ttcatgacga acggcagcat gatgcaggag gtatggccat gcgggatgtc gaacacagcg
     2341 cccagcacgt agccgatgcc gtggctcgcc cccatcggta cgccgctcgc cagaggcccc
     2401 atcgacaacc aggagcccat ttgacaatcc agccgcgcct gcaagtcgga cggatcggct
     2461 ttcactcggg ccaggccgct ggtcagcagg gtcaggccac gcaaaccttg ggcgtccgcg
     2521 taggggtttg cctcctttga gcatacgccc tccacgcaat gatccactgc ccggatcccg
     2581 gtggacagga acagccactc cggcgtgtgc accgttatgg ccgggtcgag gatgaccgcg
     2641 gccgggatga tgcgggggtg gcgaaacagc tccttcaccc gggtgcgctc gtccgttacg
     2701 ccggccaatg cactgaactc cccagcggac agcgtcgttg ggacgctgac ctggcgcacc
     2761 acgggcggat tgcaatctgg cgcgaccatc gcaccgttcg gccccttgac cggacgcagc
     2821 gcatccagcg cctcggccga ccggatgtcg tttgccaggc acagctgcac caccttcgcc
     2881 gcgtcggtga tcgatccgcc gcctaccgtc acgatcaggt ccgcaccgac cgctctcgcc
     2941 tgttcggccg cggcgatgac ggcgtggcgt ggggaatgcg ccggcatccg gtcgaacagg
     3001 ccagcgcaac ggttaccgag ggcgcctcgt acgtgtgcga tctctggcgt cgtccgattg
     3061 agggtgccgc tgaccatcag gaaaaccctc tcggcgccga accgcgtggc ttgttcggca
     3121 acggcctcgg cggcgggcac gccgaagcgc acctcgtcca tctgactgaa cacgacccgg
     3181 ccattgatcg tcatggcgcg ctttcccccg gtttgcggtt gtctcgcact ataaggaagt
     3241 gcgggcgtgg tgccagggtg cgcgacaggc ggcagggaca tggacagccg cgcccgcgtc
     3301 agcggatggg acaagacgac gatcccggcg gaatgccgga gccgccatca cgctcatcgg
     3361 gcggtttgat acgttctctg ctgtcccagg cagccctgaa catgttctgg tcacaccacc
     3421 gtctccggca cgaccgggcg gcatcgaatc cagcccgcat ccaacgcgat ggccagccgt
     3481 ttcggaagaa agcattatct atttgtaact gtgtatcaat ccagcagggt tgaccctgct
     3541 cgctcctccg g
//
LOCUS       DASWQN010001234         4033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2101665, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001234 DASWQN010000000
VERSION     DASWQN010001234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101665"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            588..1760
                     /locus_tag="VGC82_20945"
     CDS             588..1760
                     /locus_tag="VGC82_20945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEY0425798.1"
                     /translation="MSEACIVGWAHTPFGKLEDPDIESLIGRVAGAAIADAGIAPHDI
                     EGTFVSLFNNGFSAQDFPASLVLQHVPELRFKPITRYENACASGSAAVHGARDFLLSG
                     RGRFALVIGVEKMTSTPGKEVGENLLKASYQKEESEIPAGFAGVFGRIAETYFQRYGD
                     QSDALAAIAAKNHKNGVDNPFAQMRKDLGFDFCRNVSEKNPYVAPPLKRTDCSLVSDG
                     AAALIMADEETARAMGKAVRFKAAVQVNDFLPISKRDITGFEGAAHAWAQALRSSDTK
                     LSDLSFVESHDCFTIAELLEYEAMGLTPKGQGARAVLEGWTAKDGRLPINPSGGLKAK
                     GHPIGATGVSMHAITAMQLTGQAPGGMQLPKADLGAVFNMGGAAVANYVSILEPLR"
     gene            1760..3304
                     /locus_tag="VGC82_20950"
     CDS             1760..3304
                     /locus_tag="VGC82_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEY0425799.1"
                     /translation="MPTANLASMLIQTARRFPDRPAIVWRDKTWNWASFLDRVQCSAA
                     ALTAQGVRHGDRVLLHARNSNAVLETMFASWMIGAVWVPTNFRLTPPEVAYLAQSSGA
                     AVHIFDAAFPDHAAVARAENSACRLEISIGGDGAADWEAFAGYKARLDRPADVDRDHP
                     AWFFYTSGTTGRPKAGVLTHGQMDYVVCNHLCDLMPATTEHDASLVVAPLSHGAGIHA
                     LPQVARGAATILLSGERLDCEEAWRLVERHRVTNMFTVPTILTMLTRHDSVDRYDHSS
                     LRYVIYAGAPMYRADQLYALRKLGKVLVQYFGMGEVTGNITVLPAALHSENDADMPVG
                     SCGFPRTGMDVAILDDTGEPVQAGTTGEICVRGPGVFAGYHDNPEATAKATVHGWFHT
                     GDLGHMDERGFVYITGRASDMYISGGSNVYPREIEEALLLHPAVAEACVVGLPHDRWG
                     EAGVAVLVLEPGTSASAGELLAHLDGRLAKYKWPREFVFWPELPKSGYGKVTKRDVKH
                     LLQRDM"
     gene            3304..3663
                     /locus_tag="VGC82_20955"
     CDS             3304..3663
                     /locus_tag="VGC82_20955"
                     /inference="COORDINATES: protein motif:HMM:NF020457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HEY0425800.1"
                     /translation="MAASPTDPARISDYHMHVYYDPESRERAAQLRAWVEERFPVRMG
                     SWHDEPVGPHVTAMYQIAFQPELFPTLVPFVMMNRMGLTVLLHPQSGRPRDDHTINAT
                     WMGEVLPVKTEVLREVG"
BASE COUNT          744 a         1186 c         1290 g          813 t
ORIGIN      
        1 tttcagtaat tttttcgttg cgcttgtctg ctgtccgaac gagcttctga aacgatgctt
       61 cgagctcgat ggtgttaagc attcctgatt acgcattatc ctcagccgaa tttggacatc
      121 gcaccaatcg cgcaaaatca acgttgtagg ggtttcttca aagcccctgg ctcgcccgcc
      181 gtccgcgccc ggccggatca ttgaccaggt gggcccgcct gaaaggcacg tttaccagac
      241 cgaaattgtg gatttacaag cgcttgtgga agcggtcatg gctgaggatg gtgcgtaatc
      301 aggaaaggaa ttataccgac gacccttgat gttgatccgt tgccgggtcc acttccgttg
      361 gtatcttctt gattcggaat ttgttaccta gatcgcaagg atcagggtac tttgtgcctt
      421 cttggtgaaa cttgctccca ttcacgagcc ggaacgcgac acttcatatt tcctactcca
      481 agcagccgtt cagccggttg ccctgatgca aagccctcct gccctcgcct tcgccctcat
      541 gatccgctag aattcctggc taaaccccac tccaggaggg accgcttatg agcgaagctt
      601 gcatcgtcgg atgggcccat acgcctttcg gcaaactgga agatccggac atcgaatccc
      661 tgatcggccg ggttgccggt gcggcgatcg ccgatgcggg gattgctccg cacgacatcg
      721 agggcacctt tgtcagcctg ttcaacaacg gcttctcggc gcaggatttc ccggcatcgc
      781 tcgtgcttca gcatgtcccg gagctgcgct tcaagccgat cacccggtat gagaacgctt
      841 gcgcctccgg ctccgccgcg gtgcacggcg ctcgggattt cctgctgtcg ggccgcggcc
      901 gctttgcctt ggtcatcggc gtcgagaaga tgacttccac gccaggcaag gaagtcggcg
      961 aaaacctgct caaggccagc taccaaaaag aagagtcgga aattccggcc gggttcgccg
     1021 gggtgttcgg ccggatcgcg gagacgtatt tccagcgcta cggcgaccag tccgacgcgc
     1081 tggcggcgat tgcggcgaaa aaccacaaga acggggtgga caacccgttc gcgcagatgc
     1141 gcaaggatct cgggttcgat ttctgccgca atgtcagcga gaagaacccg tacgtggcgc
     1201 cgccgctgaa gcgcaccgac tgctccttgg tgtccgacgg agccgcggcc ttgatcatgg
     1261 ccgatgagga aaccgcccgg gcgatgggca aggcggttcg tttcaaggct gctgtgcagg
     1321 tcaacgactt cctgccgatc agcaagcgcg acatcaccgg gttcgagggt gccgcccatg
     1381 cctgggcaca ggccctgcgc tcgtccgaca ccaagctttc ggacctttcc ttcgtcgaat
     1441 cgcatgactg ctttaccatc gcggaactgc tggaatacga ggcgatgggc ctgacgccga
     1501 agggccaggg cgccagggcg gtgctggaag gctggaccgc aaaggatggc cgcctgccga
     1561 tcaatccgtc gggcgggctg aaggcgaagg gccaccccat tggcgcaacg ggcgtgtcga
     1621 tgcacgcgat caccgcgatg cagcttaccg gccaggcgcc gggcgggatg caactgccga
     1681 aggcggacct tggggcggta ttcaacatgg gtggcgccgc ggtggccaac tatgtctcga
     1741 tcctggagcc gctgcgctga ttccaaccgc caacctggcg tcgatgctga tccagaccgc
     1801 ccgccgtttt cccgaccggc cggcgatcgt ctggcgtgac aagacctgga actgggcctc
     1861 gttccttgat cgggtccagt gttccgccgc cgccttgacg gcgcagggtg tgcgtcatgg
     1921 cgaccgggtg ctgctgcacg cgcggaactc caacgccgtg ctggaaacga tgttcgcgtc
     1981 gtggatgatc ggcgctgttt gggtgccgac caacttccgc ctgacgccgc ccgaggtggc
     2041 gtacctggcg caatcctccg gtgcggccgt ccacatcttc gatgccgctt tcccggatca
     2101 cgccgccgtc gcgcgggcgg aaaattccgc ctgccggctg gagatttcca ttggcgggga
     2161 cggtgcggcg gactgggaag cgttcgccgg atacaaggcc aggctggatc ggccggcgga
     2221 tgtggatcgc gaccatccgg cttggttctt ctatacatcc ggcacaaccg ggcgtccgaa
     2281 ggcgggcgtt ctgacgcatg ggcagatgga ttatgtcgtc tgcaaccatt tgtgcgacct
     2341 gatgccggcc accacggagc atgacgcttc gctggtggtc gcaccgctgt cgcacggcgc
     2401 gggaattcat gcgctgccgc aggtcgcgcg cggtgccgcc acaatcctgc tatccggcga
     2461 gcggctggat tgcgaggagg cctggcgttt ggtcgaacgg caccgtgtga ccaacatgtt
     2521 caccgtgccc acgatcctga cgatgctgac ccggcatgac tcagtcgatc gttacgacca
     2581 tagttcccta cgctatgtca tctacgccgg tgcgccgatg taccgcgccg atcagcttta
     2641 cgccttgcgc aagctgggga aggtgctggt gcagtatttc ggcatgggcg aggtcacggg
     2701 caacatcacc gtcctgcccg ctgccctgca cagcgagaac gacgcggaca tgccagtggg
     2761 aagctgcggt tttccgcgta caggtatgga tgtcgcgata ctggacgata ccggcgagcc
     2821 ggtgcaggcc gggaccaccg gcgaaatatg cgtccgtggc ccgggcgtct tcgccggcta
     2881 tcatgacaac ccagaagcga cggcgaaggc caccgttcat ggctggttcc ataccggcga
     2941 cctcgggcac atggacgaac gcggctttgt ctatattacc ggaagagcgt cggacatgta
     3001 catctccggc gggtcgaatg tttatccgcg ggaaatcgag gaagcgctgt tgctgcatcc
     3061 ggctgtcgcg gaagcctgcg tggtcgggtt gccgcatgat cgttgggggg aggccggtgt
     3121 ggcagtgctg gtgttggagc caggcaccag cgcatccgcc ggggaacttc tggcgcatct
     3181 cgatggcagg ctggccaagt acaaatggcc gcgggaattc gttttctggc cggagttgcc
     3241 gaaatcgggc tacggcaagg tgaccaagcg cgatgtgaaa cacctgttgc agagggacat
     3301 gtgatggctg cctcacccac cgatccagca cgaatttcgg actaccacat gcatgtttac
     3361 tatgatcccg aaagccggga gcgggctgct caattgcggg cttgggtgga ggagcggttt
     3421 ccggtgcgga tgggaagctg gcacgacgag ccggtcgggc cgcacgtgac ggcaatgtat
     3481 cagatcgcgt tccaacctga acttttcccg acgctggtgc cgttcgtgat gatgaaccgg
     3541 atgggattga cagtgctgct gcatcctcag tccggccgcc cgcgggacga ccacacgatc
     3601 aacgcgacat ggatgggcga ggtattgccg gtgaagaccg aggtgttgcg ggaggttggc
     3661 tgaccttggg tcaggccaga agcgacgcca aggcttcgct ctaatgttga cgtagaaact
     3721 gataacgacg cccgtaatag tggtggcgga gttcccagat cataattgcc ggaacgtgtg
     3781 tttagtgcgt ttcaccatga ctggaagcgg tgttgactgg gaacggtgtt ctcttgtggc
     3841 tgcgaaggaa atcgtggatg gccgtgccca gcacgtccat ccacatgaac gtcaggcggc
     3901 aacctggctc gctctttttc cgaccagcct gaaacgcttt agtcgaacgg cagcccaaca
     3961 tagttttccg ccagcgatcg tgctgaggcc gtggacgtgc gaacaaggtc caggtcggcg
     4021 aagtggatgc gtt
//
LOCUS       DASWQN010001235        12579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1705169, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001235 DASWQN010000000
VERSION     DASWQN010001235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 12579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12579
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705169"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="VGC82_20960"
     CDS             complement(<1..1502)
                     /locus_tag="VGC82_20960"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0425801.1"
                     /translation="MKQNATALAAAALSILITGASGASAQKSGGTLRVSHRDSPASMS
                     IHEEGTVGVIMPMMGVFNNLMIYDQHIAKNSIATIVPELATDWAWNADSTALTLKLRD
                     GVRWHDGRPFTARDVKCTFDLLSNQGKEKLRLNYRESWWVNIAGTSTNGDHEATIHLK
                     QSQPALLALLASGETPVYPCHVAPRDMRQTPIGTGPFKFAEYKPNQTIKVVRNPDYWK
                     PDRPYLDAVEYTIMANRSTALLAFAAGKIDMIFPNEVTVPLLGDVKAQAPQAICQIAP
                     TSEAVGVLVNRTAPPFDNPDLRRAIALTLDRKSFIDILGNGEGNLGGVMQPPPEGAWG
                     LSPEMLADLPGYGGDVRANREEARAIMQSLGYGPDKRLPLKLSVRNLSVYRDPGNLLI
                     DQLREIYMDGELELTETATWVPKLIRKDYKIALNVLGAAVDDPDVVLFQNYVCGSARN
                     YTGHCNKQLDGMINQQSMEADPAKRKLLVQKIDHILQEELARPILYHRRAG"
     gene            1953..3149
                     /locus_tag="VGC82_20965"
     CDS             1953..3149
                     /locus_tag="VGC82_20965"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0425802.1"
                     /translation="MFGRKAGCALFALLLGLSAIPAQAADTPGVTATEIKIGQNAAFS
                     GPASVYGQISTAESHYFQMINDQGGINGRKINLIALDDAYSPPKAVEVIRRLVEDEQV
                     AILFQTLGTASNVAIRKYVNQKKIPDIWIGSGASVFSASSKDFPWTIQFQPSYRVEGQ
                     MYAKYILKTKPDAKIGILYQNDDLGRDYVAGLKDGLGDKFDKMVVRNISYEITDPTID
                     SQIVDLKSSDADVFYDASTPKFSAMSIRKTSDLDWHPLHIIDSNGALVRPALDSAGFD
                     KSVGVMTAQYLKDPTDPGWANDPGMKDFLAWKVKYAPEADVSNPVWYYGYTMAQALVV
                     VLKQAGNDLSRENIMKAATHLPDTTTLPLVLPGIKISTSPTDYQAMKGMRLVQFDGKM
                     FRLVPD"
     gene            complement(3238..4494)
                     /locus_tag="VGC82_20970"
     CDS             complement(3238..4494)
                     /locus_tag="VGC82_20970"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0425803.1"
                     /translation="MSELTETSVRAEVRAWLEANWSVDNGLVEWRHKLIDSGWGVPTW
                     PKDWYGRDLPIALQSVVEDEFRRIGAIGVAKTGIRRLAAATLLAHGTPAQKEKFLRRS
                     LSGEDTWCQLFSEPGSGSDLAGAATRAEFKGNRWVINGQKVWTTSAHHADWGLLLART
                     DWNVPKHQGLSYFIIDMHQPAIEVHPLRQMNGHASFNQVFFTDAEVPPEYLVLDVGRG
                     WAVATTTLMHERREADGIRSWGQASDRKGRIYDEERGETATVMEPYKWYPQRAGRVDL
                     VIERAKATGRNTDPVVRQEIAKLLTLSKSAEWTAHRARAAQMQGRPQGPEGSLGKLAS
                     SHVARAAARVHTLISGADAMLAGEDGPMNGLIAEILISVPAGSIAGGTDEIQRNIISE
                     RVLGMPKEPRTDNDRPFRDVPKNVVG"
     gene            complement(4511..5641)
                     /locus_tag="VGC82_20975"
     CDS             complement(4511..5641)
                     /locus_tag="VGC82_20975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0425804.1"
                     /translation="MQFSFTSEQEEFRSVLRRFLEDRSPTTVVRRLMETETGWQRENW
                     RELNQQLGLSAVHIPEAYGGQGFGHVELGIVLEEMGRALFCAPYFASTVLAATAIMNA
                     GTDGQKRELLPAIADSDTIATLAFTEPNGRWDTAGVTTTAMAAGGRFRIDGVKSFVLD
                     GHTADLIVVLARRPGSTGDDGLSFFTVPGDAPGLTRRALKVLDPTRKQARLEFRSVEA
                     ELLGAEGGGAAPFAKTMMQAAVGLANEMVGGAERLRQSALDYANLRVQFGRTIASFQS
                     MKHRQADMLVDVELAKSAAYYAASAAAADDPELPALASLAKACASDAYMQTAISTIQI
                     HGGIGFTWDNDTHLWFKRAKSSEVFLGDPGYHRELMMRAWNV"
     gene            6126..7784
                     /gene="groL"
                     /locus_tag="VGC82_20980"
     CDS             6126..7784
                     /gene="groL"
                     /locus_tag="VGC82_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423234.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0425805.1"
                     /translation="MAAKDVRFGGDARQRMLRGVDILADAVKVTLGPKGRNVVLDKSF
                     GAPRITKDGVTVAKEIELADKFENMGAQMVREVASKSSDVAGDGTTTATVLAQSIVRE
                     GAKAVAAGMNPMDLKRGIDKAVTAIVEELKSHTRKITTPAETAQVGTISANGDTEIGK
                     MISEAMQKVGNEGVITVEEAKGIQTELDVVEGMQFDRGYVSPYFVTNAEKMTADLDQP
                     YILIHEKKLSSLQPMLPLLESVVQSTRPLLIIAEDVEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAILTGGQVISEDLGIKLESVTLNMLGRAKTVLIEKENTTIVE
                     GAGSTEDIKGRCNQIRAQVEETTSDYDREKLQERLAKLAGGVAIIRVGGSTEVEVKER
                     KDRVDDGLHATRAAVEEGIVPGGGVALARASLVLSKLVAVNDDQRFGIEIVRKAVLTP
                     LRQIAENAGEDGAVVSGRVLDRDEYNWGFDAQSGQFKDLVAAGIIDPTKVVRSALQNA
                     ASVAGLLVTTEAMVAERPEKAAPMPQGGGMGGMGGMGGMGDMGF"
     gene            7938..8240
                     /locus_tag="VGC82_20985"
     CDS             7938..8240
                     /locus_tag="VGC82_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425806.1"
                     /translation="MADLQKLLFDTKARISDTEILAAARLNACVRVFAGRQLSDDLIE
                     HINEAVSKEATQLIAEGVLRERPDWLAVIVGRRLHVAFGSHAVQQLCRDLRDAGAV"
     gene            complement(8380..9354)
                     /locus_tag="VGC82_20990"
     CDS             complement(8380..9354)
                     /locus_tag="VGC82_20990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0425807.1"
                     /translation="MKVGLFINTQFPEGFNVADRVPEIVEQVRTARDAGFSSLWFPHH
                     WLTYPMQMLQITPVMGYVAAHAQGMTIGPNILILPPLNPMHVAEEAATLDVLTGGNYI
                     LGIGLGYRQPEFDAFGIPLSERAPRFNESIGLMKRLWTEDRVSHKGRFYTVNDAGIGV
                     KPVQAGGPPLYIAGQADVSVRRAARIGDAWLIVNSGGLSKVMPLMNTYRAALAEYGRT
                     AREFPITVECYVGDRHSTAHEECREPLEYKYNAYASWGLQGRRTETNFEDFARDKFII
                     GDKVSVKEEIARYRELLGVDHFIMRCNWPGLPQDKTLATIRRLGEIFG"
     gene            complement(9487..10332)
                     /locus_tag="VGC82_20995"
     CDS             complement(9487..10332)
                     /locus_tag="VGC82_20995"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0425808.1"
                     /translation="MSVEVIPTDAAVGAEILGVDLARPLDDASFAAIELAYNDHGVIF
                     FRDQHITPAQQVAFTRRFGDIEFNIFGERWSVPGSPEIVVVSNVTEDGRPIGVRRAGE
                     NWHSDMCYAARPPRGTMLYALEIPDLHGLTLGDTEFASAAAAWEALPEPMRHFLEGRG
                     AVFDFSGRKRAFPLSQAERDRYPPVRHPIVRTHPFTGRKCLYVMRDDCVGIEGMDEPE
                     AEALIAALADHIVKPEFIYRHRWRRGDLLMWDNCTVQHRAIQDYDMPRRRLMHRTTMG
                     GAVPV"
     gene            complement(10435..11313)
                     /locus_tag="VGC82_21000"
     CDS             complement(10435..11313)
                     /locus_tag="VGC82_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0425809.1"
                     /translation="MNFRRDARPRRDFLQWLWVLAVLALPLPAWATDLKIATWNLDWL
                     TTRQAGHGLPADVVPRDEDDFARLAQYARELNADVVAIQEVDGFQAATKVFPRDLYSI
                     HMTRDHVMQRVGIVVRRGLKYDMNPDVTALAMNHLRSGADITLHLGSSDLRILAVHLK
                     TGCRNQPLPRTHDRSCLELRDQVPVLEEWIAARRQEGMAFVILGDFNRWMDGRDALLS
                     DLRRAAPLVRATEGHSSPCWGAENFIDHILAGGDAANWMQPDTLRVMTYRETDRALKD
                     RLSDHCPVSVKFVAPD"
     gene            complement(11460..>12579)
                     /locus_tag="VGC82_21005"
     CDS             complement(11460..>12579)
                     /locus_tag="VGC82_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0425810.1"
                     /translation="LRFVPYIGAPLAALLPLALAAAVSPDWSMMLWTAGLYAGTEAIL
                     GQVVEPLLYGRSTGLSPFSVVVSATFWTWLWGPIGLILSTPLTLCLVVLGRHVERLEF
                     LDVILGDRPALTPVESFYQRMLAGDPDEASDQAEILLRDRPLSSYYDEVALKGLQLAA
                     NDAARGVLTEGQLERIKASMQELIDDLDEHDDKDPETKEALESATTAPPRGEQDIPRH
                     PAPGSVAEDTLPPAWRGPAPVLCIAGRGPLDEVAASMLSQLLHKNGLRARVLPHEAAS
                     RVRIGSLEGDGVAMVCMCYLELGGAPSHLRYLLRRLRQRLPAARLLVGLWPAEQAILT
                     DDRLRAAVGADVYTSSLREAVEACLEAAREEIRPVAAA"
BASE COUNT         2336 a         3975 c         3924 g         2344 t
ORIGIN      
        1 ccggcgcgcc ggtgatagag gatcggccga gccagttctt cctgcaatat gtggtcgatt
       61 ttctgcacca gtagtttacg cttggcggga tcggcttcca tcgactgctg gttgatcatt
      121 ccatccaatt gtttattgca gtgtcccgtg taattccgag cggaaccgca gacgtagttc
      181 tggaacaata cgacgtcggg atcatcgacg gcggccccca gcacgttaag cgcgatcttg
      241 tagtccttgc ggatcaactt cggcacccag gtcgcggttt ccgtcagttc cagctcgcca
      301 tccatgtaga tttcgcgtag ctggtcgatc agcaggttgc cgggatcgcg atacactgac
      361 aggttgcgaa cggatagttt caatggcaac cgcttatccg gcccatagcc cagactctgc
      421 atgatggcgc gggcttcctc gcggttcgcg cgaacatcgc cgccatagcc aggcaggtcc
      481 gccagcatct cgggcgacag gccccaggct ccctccggcg gaggttgcat cacaccgcct
      541 aggtttccct cgccgttgcc gagaatgtcg atgaagctct tgcggtccag cgtgagtgcg
      601 atcgctcggc gcagatccgg gttgtcgaac ggtggcgccg tccggttgac gagcacgccg
      661 acggcttcgc tggtcggtgc gatctggcag atggcctgcg gcgcctgcgc ctttacgtcg
      721 cccaggagcg ggaccgtgac ctcattggga aagatcatgt cgattttccc cgcggcgaac
      781 gccagcagcg cggtcgagcg gttggccatg atggtatatt cgaccgcatc gaggtacggc
      841 cgatccggct tccagtagtc cggattgcgc accaccttga tggtctggtt gggcttgtac
      901 tccgcgaact tgaacgggcc ggtgccgatt ggcgtttgcc gcatgtcacg cggggccacg
      961 tgacagggat aaaccggggt ttcgcccgat gccagcaagg ccagcaatgc cggttgcgat
     1021 tgtttcagat gaatcgtcgc ctcgtggtcg ccgttggtgc tcgtgccggc gatgttcacc
     1081 caccaacttt cgcggtaatt aagccgcagc ttttcctttc cctggttgct cagcaggtcg
     1141 aaggtgcatt tgacgtcgcg tgcggtgaag ggccggccgt catgccagcg gacaccgtcc
     1201 cgaagtttca gcgtaagggc ggtgctgtcg gcgttccatg cccaatcggt cgcaagctcc
     1261 ggcacgatcg tcgcgatgct gtttttcgcg atgtgctggt cgtagatcat caggttgttg
     1321 aacacgccca tcattggcat gatgacgccg acggtgcctt cctcgtggat ggacatgctg
     1381 gcggggctat cgcggtggga gacgcgcaag gtgccgcccg acttttgcgc gctggcgccc
     1441 gatgcgccgg taatcaggat cgagagtgca gccgcggcca atgccgtagc gttttgcttc
     1501 atgacacgct cccctgggaa ttttcccgcg ccggcgacgc ctttaatctt ggtggccgga
     1561 aagcgaccgt atccgggtga tggcggcaag cacaagcgcg ctcctgataa tggcttgagc
     1621 gtcccggcct ctgcgatcgc ccaggctgtc atggccgaga ctcccgcact tggaatgatg
     1681 ccaggcgagg gtgagcggcg gacttcaccg ccattcagcg cttaatcaac gttcccactc
     1741 gtttgtaatg cccggtttgg tcggcgggct gcgacctggg ttacccgaaa tgcctgactg
     1801 agcccagaac atatctcacg ccgtatccac gatacttgga gttgaagcat tcccagctcc
     1861 gacaacacag cgaatggtcc ggatgccgca atggatgctt gccgttaatc cgggcatcaa
     1921 ccacactgcc gctaatattg gggagaaacg caatgttcgg acggaaggcg ggctgtgccc
     1981 ttttcgcgct gttgctgggt ctttcggcca taccggcgca ggcggcggac acaccagggg
     2041 tcactgccac cgaaatcaag atcggccaga atgccgcctt cagcggcccg gccagcgttt
     2101 atggtcagat ttcgaccgcg gaatcgcact acttccagat gatcaacgac cagggcggca
     2161 tcaacggccg caaaatcaat ttgatcgccc tggatgacgc ttatagcccg cccaaggcag
     2221 tcgaggtcat acgccgattg gttgaagacg agcaggtcgc catcctgttc caaacgttgg
     2281 gtaccgcgtc gaacgtcgca atccgaaaat acgtcaatca gaagaaaatt ccggatatct
     2341 ggatcggctc gggtgcctcg gtgttttccg ccagttcgaa agacttcccg tggaccattc
     2401 agttccagcc gagctatcgg gtggaagggc agatgtatgc gaaatacatc ctgaaaacca
     2461 aaccagatgc gaaaatcggc attttgtacc agaacgacga cctgggccgg gactatgttg
     2521 caggcctgaa ggacggcctg ggcgataagt tcgacaagat ggtcgtcagg aatatttcat
     2581 acgaaatcac cgatcccacc atcgattccc agatcgtgga tctgaagtcg tccgatgccg
     2641 atgttttcta cgacgcaagc acgccgaaat tctccgccat gtcaatccgg aagacctccg
     2701 atctcgactg gcatccgctg catatcatcg actctaacgg ggcgctggtg agaccggcgc
     2761 tggattccgc gggtttcgat aagtcggtgg gcgtcatgac ggcgcaatat ctgaaagacc
     2821 caaccgatcc aggttgggcc aacgatcccg gcatgaagga tttcttggcc tggaaggtga
     2881 aatatgcgcc cgaggctgat gtctccaatc ccgtctggta ctatggctac acgatggcgc
     2941 aagcgctggt cgtggtgctg aagcaggcag ggaacgatct gtcccgcgag aatatcatga
     3001 aggcggcaac tcatctgccg gataccacca ctctgccgct ggtcctgccc ggtatcaaaa
     3061 tcagcacgtc gcctaccgat taccaggcga tgaaaggaat gcgcctggtg cagttcgacg
     3121 gcaaaatgtt caggttggtg ccggattagg cgcgaccggg tgcctcgcca tgacactctc
     3181 ggttgaaaca actccggtct ggccgcgtgg ctgacgccgc cgcggccatt gacccgatca
     3241 ccccaccaca ttcttcggca catcccggaa cggccggtcg ttgtccgtac gtggttcctt
     3301 tggcatcccc agcacccgtt cggaaataat gttccgctgg atttcatccg tcccgccggc
     3361 gatcgacccc gccggcaccg agatcaggat ctccgcgatc agcccgttca tcggtccatc
     3421 ctcgccggcc agcatcgcat ccgcgccgct gatcagcgtg tgcacccgcg ccgccgcccg
     3481 tgccacatgg ctgctcgcaa gcttaccgag cgaaccttcg ggtccctgcg gccggccctg
     3541 catctgcgcg gcgcgtgccc gatgcgcggt ccattccgca gacttcgaaa gggtcagaag
     3601 cttggctatt tcctgccgca ccaccgggtc tgtgttccgc cccgtggcct tagcccgctc
     3661 gatcaccagg tccacccggc cggcgcgttg cggataccac ttgtacggct ccatgacggt
     3721 ggcggtctcg ccacgctcct cgtcgtagat ccggcccttg cggtcggacg cctgacccca
     3781 ggagcggatg ccgtccgcct cccgccgttc gtgcatcagt gtcgtcgtcg ctaccgccca
     3841 gccccggccg acatccagca ccaggtactc cggaggcact tcggcgtcgg tgaagaacac
     3901 ctggttgaag gatgcgtgcc cgttcatctg ccgcagcggg tgcacctcga tggctggctg
     3961 atgcatgtcg atgatgaagt aagacagccc ctggtgcttg ggaacgttcc agtcggtgcg
     4021 tgccagcagc aggccccaat cggcgtggtg cgcactagtg gtccagacct tctggccgtt
     4081 gatcacccag cggttgccct tgaactcggc ccgcgtcgcg gcgccggcca aatccgatcc
     4141 gctgccgggc tcgctgaaca actggcacca agtgtcctcg ccggacaggc tgcggcgcag
     4201 gaatttctcc ttctgcgcgg gcgtgccgtg cgccagcagc gtggccgcgg ccaggcggcg
     4261 aatgccggtc ttggcgacgc cgatcgcccc gatacgccgg aactcgtcct cgaccaccga
     4321 ttgcagggcg atcggcaggt cccggccgta ccagtccttc ggccaggtcg gcacgcccca
     4381 gccggagtcg atcagcttgt ggcgccattc caccaggccg ttatcgacgc tccagttcgc
     4441 ttccagccag gcgcgcacct cggcgcggac ggaggtttct gtcagttcgc tcatgctcgt
     4501 tccctccggt ctagacattc caggcgcgca tcatcagctc gcgatgatag cccggatcgc
     4561 cgaggaacac ctcggagctc ttcgcgcgct tgaaccacag gtgggtgtcg ttgtcccagg
     4621 tgaagccgat gccgccatgg atctggatcg tactgatcgc cgtctgcata taggcatccg
     4681 acgcgcaggc cttcgccaat gaggccagcg ccggcagctc agggtcgtcc gcggccgcgg
     4741 ccgaagcggc gtagtaggcg gccgacttcg ccagctcgac atcgaccagc atatcggcct
     4801 gcctgtgctt catcgattgg aacgaggcga tggtgcggcc aaactgcacc cgcaggttgg
     4861 catagtccag tgccgactgg cgcagccgtt cggcgccgcc gaccatttcg ttcgccaagc
     4921 cgacagcggc ctgcatcatc gtcttcgcga atggcgccgc cccgccgccc tccgcaccca
     4981 gaagctcggc ctcgaccgat cggaactcga gccgcgcctg ctttcgggtc gggtcgagca
     5041 ccttgagcgc ccggcgggtc agccccggcg cgtcacctgg cacagtgaag aacgagagcc
     5101 catcgtcgcc ggtggaaccg gggcggcggg ccagcacgac gatcagatcg gccgtgtgac
     5161 cgtcgagaac gaaactcttg acgccgtcga ttcgaaacct gcccccggcg gccatcgccg
     5221 tcgtcgtgac cccagccgta tcccaacgcc cattgggttc ggtaaaggcc agagtggcga
     5281 ttgtgtcgct gtcagcgatg gcgggcagca attcacgttt ttgcccgtcg gtcccggcgt
     5341 tcatgatcgc ggttgccgcc agcaccgtcg aagcaaaata gggcgcgcag aacaaggctc
     5401 ggcccatctc ctccagcacg atgccgagct cgacgtgacc aaaaccttgg ccgccatagg
     5461 cttctgggat gtggacggcg gacaggccga gctgctgatt gagttcgcgc cagttctcgc
     5521 gctgccagcc ggtctcggtc tccatgagcc gccggacgac ggttgtcggc gacctgtctt
     5581 cgaggaaccg tcgtagtacc gaacggaatt cctcctgctc gctggtgaag ctgaattgca
     5641 tgccgtctgc tcccggtacg ttttctgcgc tgatcagttt ctgcgctggt caggctgtcg
     5701 cgtgtgcgca actccacagt gcaggacgat gcagcagctc tgcaaggcgc gctgccccaa
     5761 gggctgccct taccacccat cgaatcaatt cgacggcagg ttcgcctcgg tccggttgac
     5821 gtggccggca ccaattcgta ggtcattggc actccttgga tgggagtgcc aatttcaagg
     5881 acaatggcgt ctcgcgatcg agccgcggtt cgcaggagga cttcatgaac gtccgtccac
     5941 tgcatgacgg gctgttatcc gccttgtggc cgccgaggaa aatccagcga tggcatcacc
     6001 attttggata tcgcaaagcc gatgcagccg cgaagtcgtc gcggcagggc ccggccatca
     6061 ctctgcctgc tggcaagact cgatttcgtt gacacgatct aacggttgtt gaggagggaa
     6121 agcacatggc tgcaaaagac gttcggtttg gcggggacgc tcgccagcgc atgcttcgcg
     6181 gcgtggacat tcttgccgac gcggtaaagg tcacgcttgg tcccaaggga cgtaatgtcg
     6241 tgctggacaa aagtttcggg gcgccgcgga tcaccaagga cggcgtcacc gtggccaagg
     6301 aaatcgaact cgccgacaag ttcgagaaca tgggcgcgca gatggtgcgc gaggtggcca
     6361 gcaagagcag cgacgtggct ggcgatggaa ccaccactgc caccgtattg gcgcagtcga
     6421 tcgtgcgcga gggtgcgaag gccgtcgcgg cgggcatgaa cccgatggac ctgaagcgcg
     6481 gcatcgacaa agcggtgacc gccattgtcg aggaactgaa gagccacacc cgcaagatca
     6541 ccactccggc cgagaccgca caggtcggca ccatatccgc gaatggcgat accgagatcg
     6601 gcaaaatgat ctctgaggcg atgcagaagg tcggcaatga aggcgtgatc acggtggagg
     6661 aggccaaggg gatccagacc gagctagatg tcgtcgaagg tatgcagttc gaccgtggct
     6721 acgtctcgcc ttacttcgtc accaacgcgg agaagatgac ggcggacctc gaccagccct
     6781 acatcctgat ccacgaaaag aagctttcca gcctgcagcc gatgttgccg ctgctggaaa
     6841 gcgtggtgca gtcaacgcgt cccttgctga tcatcgccga ggatgtcgaa ggcgaagcgc
     6901 tcgcgaccct tgtggtgaac aagctgcgtg gtggcctgaa ggtggcggcc gtgaaggcgc
     6961 ctggcttcgg ggaccgccgc aaggcgatgc tggaagatat cgcgatcctg accggcggcc
     7021 aggtcatcag cgaggatctt ggcatcaagc tcgagagcgt gacgctgaac atgctgggtc
     7081 gggcaaagac cgtcctgatc gagaaggaaa acaccacgat cgtcgaaggc gccggcagca
     7141 ccgaagatat caagggacgc tgcaaccaga tccgcgccca ggtcgaggag accacgtcgg
     7201 actatgaccg cgagaagttg caggagcggt tggccaagtt ggccggcggc gtcgcgatca
     7261 tccgggtcgg tggctctacc gaggtggagg tgaaggaacg caaggaccgc gtggacgatg
     7321 gtctgcatgc cacgcgggcc gcggtcgaag aaggcatcgt gccgggcggc ggggtcgcac
     7381 tcgcaagggc gtccctggtc ctgtcgaagc tggttgcagt gaatgacgac cagcgatttg
     7441 gcatcgaaat cgtccgcaag gccgtgctga cgcctctgcg ccagatcgcg gagaatgccg
     7501 gcgaggatgg tgcggtcgtt tccggccggg tgctcgatcg ggacgagtac aactggggtt
     7561 tcgacgctca gagtggccag ttcaaggatc tggtggccgc gggcatcatc gacccaacga
     7621 aggtggttcg atcggcgctg caaaatgccg cttcggtagc agggctgctt gtcacgactg
     7681 aagccatggt ggccgagcgg ccggagaaag cggccccgat gcctcaaggt ggcgggatgg
     7741 gtggaatggg cggcatgggc ggaatgggcg atatgggctt ctgatccggt cagcatcagc
     7801 ctggatcggc gcgaaaaggc attcgacttt gggtgggcga agtgcctttt cgtcgccggc
     7861 cgctgatctg ccctggcgtc ccgctactca ttcgtgccga tctgggattc gttgtcagaa
     7921 ggattggacg ggatgccatg gcggatttgc agaagctgct ttttgatacc aaggcaagga
     7981 tcagcgacac ggaaatcctg gcggctgccc gtttgaacgc gtgtgtcagg gtattcgcgg
     8041 gccgccaact ttctgacgat ctcatcgagc atatcaacga agcagtaagc aaagaagcga
     8101 ctcagcttat cgcggaaggt gtactacgcg aacggccgga ctggctcgcc gtcatcgttg
     8161 ggcgacgact tcatgtagcg ttcggcagtc atgctgtgca gcaactgtgc cgcgatttga
     8221 gggatgcggg cgcggtgtag ctgtcctcca caggcgcatg gcgttcttcg gctggagcgt
     8281 gagcgcgccg ggcggaacgt ccgaggcgtg tgaatcacgc gatcaaacga ctggaccata
     8341 tccccgccgt tccgcgatca ccattccacc gcaaccgctc tacccgaaga tttcccccaa
     8401 tcgcctgatc gtcgccagcg tcttgtcctg cggcaggccg ggccagttgc agcgcatgat
     8461 aaagtggtcc acgccaagca gttcccggta tcgggcgatc tcttctttga ccgaaacttt
     8521 gtcgccgatg atgaatttgt cgcgggcgaa gtcctcgaaa ttggtttcgg tgcgccttcc
     8581 ctgcaggccc caggaagcat aggcgttgta tttatactcc agcggttcac ggcattcttc
     8641 gtgcgcggtc gaatgacggt caccgacata acactcgacg gtgatcggaa attcccgggc
     8701 cgtgcggcca tattcggcca acgccgcccg gtaagtgttc atcagcggca tcaccttgct
     8761 caggccgccg ctgttgacga tcagccaggc atccccaatg cgcgccgcac gcctgaccga
     8821 gacatccgcc tggccagcga tatacagcgg cggccctcct gcctgcaccg gcttcacccc
     8881 gatgccggca tcgttcaccg tgtagaagcg tcccttgtgg ctgacccggt cctcggtcca
     8941 cagccgcttc atcaggccga tgctttcgtt gaagcgcggc gcgcgttcgg acagtggaat
     9001 gccgaacgcg tcgaactcgg gctggcgata gcctagcccg atgccgagga tgtagttgcc
     9061 gccggtcagc acatcgagcg tggcggcttc ctcggcgaca tgcattgggt ttagcggtgg
     9121 caggatcagg atgttggggc cgatcgtcat gccctgcgcg tgcgccgcga catagcccat
     9181 caccggcgtg atctgcagca tttgcatcgg gtaggtcagc caatgatgcg ggaaccagag
     9241 cgaactgaaa ccagcatccc gagcggtccg cacctgctcg acgatctccg gaacacggtc
     9301 cgcgacgttg aaaccttccg gaaactgcgt gttgatgaac agaccgacct tcatgactgg
     9361 cgtccttatg gttttagttg aaccagcgta gagcagctcc acttttcctg gaatatggcc
     9421 gttcccttgg ctgcaggccg agtcgcgggg acaaagaccc ttcagcccgg atcgctccgt
     9481 cccgatctac acaggcactg caccgcccat ggtcgtgcga tgcatcagcc gccgccgcgg
     9541 catgtcgtag tcctggatgg cgcggtgctg caccgtgcaa ttgtcccaca tcagcagatc
     9601 cccgcggcgc caccgatggc ggtagataaa ttccggcttg acgatgtggt ccgcgagggc
     9661 ggcgatcagc gcctcggctt cgggctcgtc catgccttct attccgacgc agtcgtcgcg
     9721 catgacgtac aagcattttc gcccggtgaa gggatgcgtt cgcacgatcg gatgcctgac
     9781 cggaggatag cggtcgcgct ccgcctggct caacgggaac gctcgcttgc ggccactgaa
     9841 gtcgaacacc gcacctcggc cttcgagaaa atgccgcatc ggctcgggca gcgcctccca
     9901 cgccgcggcg gcactggcga actcggtgtc cccgagcgtc aggccatgca gatcggggat
     9961 ttcgagcgca tacagcatgg tcccgcgcgg cggcctggcg gcgtagcaca tgtcgctgtg
    10021 ccagttctcg ccggcgcgcc gcacgccgat cggccgtccg tcctcggtca cgttggagac
    10081 cacgacaatc tcggggctgc cggggacgct ccagcgttcg ccgaagatgt tgaactcgat
    10141 gtcgccgaag cggcgggtga aggcgacctg ctgcgccggg gtgatgtgct ggtcgcggaa
    10201 gaagatgacg ccgtgatcgt tatacgcaag ctcgatcgcg gcaaagctgg cgtcgtcgag
    10261 cggccgggca agatcgacac ccaggatctc tgcaccgacg gcggcatcgg tcgggatgac
    10321 ttcaacggac atcgacggtc ctccccgtgg ccgaaccgcc gagacctccg gcggctggac
    10381 acccacgagc gtatcgtaat caagccggtg cgcaaccgtg gcgcgggaga tgcctcagtc
    10441 cggcgccacg aatttcaccg ataccgggca atggtcggac aaccggtcct tcagtgcgcg
    10501 gtcggtctcg cggtatgtca tcacccgcaa cgtatccggc tgcatccagt tcgcggcatc
    10561 gcccccggcc aggatatgat cgatgaagtt ctccgcaccc cagcagggag aactatgtcc
    10621 ttccgtggcc cgaaccaagg gcgcagcccg gcgcaagtcg ctcaacagcg catcgcggcc
    10681 gtccatccac cgattgaagt cacccaggat aacgaacgcc atgccctcct gccgccgcgc
    10741 ggcgatccat tcctccagca ccggaacctg gtcgcgaagt tccagacaag accggtcgtg
    10801 agtacgcggc aacggctggt tgcgacagcc ggtcttcagg tgaaccgcca ggatgcgcaa
    10861 atcggacgac cccagatgca gcgtgatgtc ggcgccgctg cgcaggtgat tcatggcaag
    10921 ggccgtaacg tccgggttca tgtcgtattt caggccccgg cggaccacga tgccgacgcg
    10981 ttgcatgacg tggtcgcgag tcatgtgaat tgagtaaagg tcgcgcggaa agactttcgt
    11041 tgctgcctgg aacccatcga cctcttgtat ggcgacgaca tcggcgttca gttcccgcgc
    11101 atactgcgca agacgagcga agtcgtcctc gtcacgcggg accacgtccg ccggcaagcc
    11161 atggccggcc tgccgcgtcg tgagccaatc gagattccag gtcgcgatct tgagatcggt
    11221 ggcccacgcc ggcaatggaa gcgccagcac ggccaacacc caaagccatt gcaggaaatc
    11281 cctgcgaggc cgcgcatcgc gccggaaatt catgacgctg gaccggattt cttcatgcgt
    11341 ccctgacttt tacgctgcat ccgtccgcgc cgccggtcag cgatcgccgc ccgcggtgga
    11401 tcgcccgctc ataacgataa ctggttaacc aaacccttct atacggctaa tggggaaaat
    11461 catgccgcgg ccaccggccg gatctcctcc cgggcggctt ccaggcacgc ctccacggcc
    11521 tctcgcagcg agctcgtata gacatccgcg cccaccgccg cgcgcagccg gtcgtccgtc
    11581 aggatcgcct gttcggccgg ccatagcccc accagcaacc gagcggcggg aagccgctgg
    11641 cgcaaccggc gaagcagata ccgaagatgg gagggcgcgc cgccaagctc caggtagcac
    11701 atgcaaacca tcgccacgcc gtcgccctcc aacgagccga tgcgcacccg ggaggccgct
    11761 tcatgaggca gtacccgggc cctcaggccg ttcttgtgca gcaactgcga caacatgctg
    11821 gcggcgacct catccaacgg accgcgtccg gcgatgcaca gcaccggcgc cggtccgcgc
    11881 caggccggcg gcaacgtatc ctcggccacg ctgccggggg cagggtgcct gggaatgtcc
    11941 tgctcgccgc ggggcggagc cgtcgtcgca ctttccagtg cctccttcgt ctcggggtcc
    12001 ttgtcgtcat gctcgtcgag gtcgtcgatc agttcctgca tcgaggcctt gatccgctcc
    12061 agttgcccct cggtcagcac cccgcgtgcg gcgtcgttgg ccgccagttg caggcctttc
    12121 agcgcgacct cgtcataata cgacgacagc ggccggtcgc gcagcaggat ctcggcctgg
    12181 tcgctcgcct cgtccgggtc acccgccagc atgcgctggt agaagctctc gaccggggtc
    12241 agggcagggc ggtcgcccag gataacatcg aggaattcca accgttcgac gtggcgcccc
    12301 agcaccacca ggcacagcgt cagcggagtc gacaggatca gcccgatcgg accccacagc
    12361 caggtccaga acgtcgccga cacgacgacc gagaacggcg acaggccggt gctgcggccg
    12421 tacagcaaag gctccaccac ctggcccagt atcgcctcgg tgccggcata caacccggcg
    12481 gtccacagca tcatcgacca gtccggactg accgcggcag ccaacgccag gggcagcagc
    12541 gcggccaacg gcgcgccgat atagggcacg aagcgcagc
//
LOCUS       DASWQN010001236         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_122027, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001236 DASWQN010000000
VERSION     DASWQN010001236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122027"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VGC82_21010"
     CDS             <1..534
                     /locus_tag="VGC82_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0425811.1"
                     /translation="REGHVLLDGKDANLLPAFERARCGIAMVPEGRGIFASLTVMENL
                     RLAANPGDWTETRVLDLFPRLGQRAGNRGNHLSGGEQQMLAIARALMTNPRLLILDEA
                     TEGLAPLMRDEIWRVIRTVGEAGVAVLVVDKTVSSVLSLADRVIMLVKGKIAWAGEPD
                     MLRADPALMHRHLGIDA"
     gene            complement(620..1141)
                     /locus_tag="VGC82_21015"
     CDS             complement(620..1141)
                     /locus_tag="VGC82_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0425812.1"
                     /translation="MDYRTQALAALVAYTLTAAAYAADVPVERGKYLTQIAGCTDCHT
                     PGHFFGHPDMTRYLGGSDVGFAVPGQGVFIGPNLTPDKETGLGNWTAQQIVTAITTGQ
                     RPDGRILAPSMPWRGLTNLTKTDALAIAAFLKSLPPVSNKVPGPFGPDEQVNVFVMSV
                     QPGRVYASQKPPK"
     gene            complement(1193..2098)
                     /locus_tag="VGC82_21020"
     CDS             complement(1193..2098)
                     /locus_tag="VGC82_21020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY0425813.1"
                     /translation="MKILFVTSNRLGDAILSTGLLDYLIHTYPAARITVVCGPVAEGV
                     FARMPNRERTIVLSKRKWGLHWLPLWAETAARFWDLVVDIRGSALSWLVPTRRRAVFS
                     RADGPKIAQLGAILGLSPPPLPVAWIGKVDRARVSVLLPEDRPIIVLAPTANWQPKVW
                     PAVRFAAAFESLAASVLPGALPVVLGGPGQAERGMADPLLVALPAALDLVGVLSLPEA
                     AAVLQKAALFIGNDSGLMHLSAAAGAPTIGLFGPTDAATYAPAGKRAVAVVAPSMNGI
                     STETVVRAARDLLADAGRPRECWSA"
     gene            2263..>2540
                     /locus_tag="VGC82_21025"
     CDS             2263..>2540
                     /locus_tag="VGC82_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0425814.1"
                     /translation="MSEETLVALGEDYPELRDSVRKICAQYPGTYWSGLEDKEAYPTE
                     FVEELTQAGFLGALIPEEYGGSGLPLRAAAVILEEINASGCVASQGHA"
BASE COUNT          463 a          817 c          815 g          445 t
ORIGIN      
        1 cgggagggcc atgtcctgct cgacggcaag gatgcgaact tgttgcccgc gttcgagcgg
       61 gctcgttgtg ggattgcgat ggtccccgaa ggccgcggca tctttgccag cctcaccgtg
      121 atggagaatc tgcgcctcgc cgccaatcct ggcgattgga ccgaaacgcg tgtgctggac
      181 ctgtttcccc ggttaggcca acgcgccggc aatcggggca atcacctgtc cggcggcgaa
      241 caacagatgt tggcgatcgc ccgggcgctg atgaccaatc cgaggctgct gattctggac
      301 gaggcaaccg aaggccttgc cccgctgatg cgcgacgaaa tctggcgggt cattcggacc
      361 gtaggcgagg cgggcgttgc ggtcctggtg gtggacaaga ctgtctcctc tgtcctgtcg
      421 ctggccgatc gcgtcatcat gctggtcaag ggcaagatcg cctgggccgg tgaacccgac
      481 atgctgcgtg ccgacccggc cctgatgcac cggcatcttg gaatcgatgc ataatcgtga
      541 caaacggcga catggatttg cattaagcgc ttggtcgccg ggtccggctg ccgcgggcac
      601 gacgcggcgc ctcgatgcgc tactttggcg gcttctggct ggcataaacc cggccgggct
      661 gcaccgacat gacgaagacg ttcacttgct catccggccc gaatgggcct gggaccttgt
      721 tgctgacagg tggcaggctt ttcaggaaag cggcgattgc caaagcgtcc gtcttggtca
      781 ggttcgtgag gccacgccac ggcatcgatg gcgccaatat gcgtccatcc ggccgttgcc
      841 cagtggtgat cgccgtgacg atctgctgcg ccgtccagtt tcccaaaccg gtttccttgt
      901 ccggcgtcag gttcgggccg atgaacacgc cctgccccgg aaccgcgaag ccgacatccg
      961 atccacccag gtagcgcgtc atatcgggat ggccaaagaa atgtccgggc gtatggcagt
     1021 cggtgcaacc ggcgatctgc gtcaggtatt tgccccgctc cacgggcacg tcggccgcat
     1081 aggccgcagc ggttaaggtg tatgcgacaa gcgccgccag cgcctgcgtg cggtaatcca
     1141 tgcgaatcct ccccattggt ctggcgattt tattctgttt gttgcttgcc gctcaagctg
     1201 accagcattc ccgaggacgt cctgcatctg ccagaagatc cctcgcggcc ctgacaacgg
     1261 tttcggtcga aatgccattc atggaagggg cgacgaccgc gacggcacgc ttgccggcgg
     1321 gcgcgtaggt cgctgcgtcg gtcgggccga acaggccgat cgttggtgcg ccggccgcgg
     1381 ccgacagatg catcagcccg gaatcgttgc cgatgaacaa ggccgccttc tgaagcacag
     1441 ccgccgcctc cggcagcgac aaaacgccga ccagatcgag cgccgcgggc agcgcgacaa
     1501 gcaacggatc ggccattccc cgttccgcct gtccggggcc acccagcacg accggtagcg
     1561 cccccggaag cacggacgct gccaagcttt cgaaggccgc ggcgaaacgc acggccggcc
     1621 acacctttgg ctgccagttt gctgtcggtg cgagcacaat aattggtcta tcctccggta
     1681 gcaaaaccga cactcgtgct cggtccactt tgcctatcca cgcgaccggc aaaggcggcg
     1741 gcgatagtcc caggatggct ccgagctggg cgatcttggg gccgtctgcg cgactgaaaa
     1801 ccgctcggcg gcgggtcggc accagccatg acaacgccga tccacggata tcgaccacga
     1861 ggtcccagaa ccgcgccgcg gtctccgccc aaagcggcag ccaatgcagg ccccatttcc
     1921 gcttggacag gacgatggtc ctctcgcggt tcggcatccg cgcgaacacg ccctcggcca
     1981 ccggcccgca aaccactgtg atgcgtgccg ccgggtaggt gtggatcagg taatccagca
     2041 ggccggttga caggatcgcg tcgccgaggc ggttagatgt cacgaacagg attttcatct
     2101 gcgacggcga ttagaccatg attgcgcggc gcgaaacgtc cgcagcggat gaaggccgtc
     2161 agaagcgggc cgaggcagcg cacctgacca tgcagcgccc cggtttgacc cgatggcaat
     2221 cgttgggcag cgtgcgcccc aatccaatca acggagaccc acatgtccga ggaaaccctt
     2281 gtcgcgctcg gcgaagatta tccggaactg cgcgactcgg tccgcaagat ctgcgcccaa
     2341 taccccggca cctactggag cggcctggag gacaaggaag cctacccgac ggaattcgtc
     2401 gaagaattga ctcaagcggg gttcctgggt gcgctgatcc ccgaggagta cggcggctct
     2461 gggctgccgt tgcgtgccgc ggcggtcatc ctggaagaga tcaacgcgtc cggttgcgtc
     2521 gccagccagg ggcatgccca
//
LOCUS       DASWQN010001237         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2496664, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001237 DASWQN010000000
VERSION     DASWQN010001237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496664"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..761)
                     /locus_tag="VGC82_21030"
     CDS             complement(51..761)
                     /locus_tag="VGC82_21030"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY0425815.1"
                     /translation="MKRKSLVRSIARRVGLHVEPLMPDLMDFLASREITTVFDVGANM
                     GQFAESLRKKGYCGRIVSFEPIKQVFAQLEAKARRDPLWTVHNCALGERAGQATINVS
                     SESVFSSILGQRHAATVHAAVSANVRTETIAVATLDDVFAEFATENCFLKIDTQGFER
                     QVIAGAARALRSLRGLQMELPVIHLYENVWTLSQAIDHMADRGFVVSQVDPVSFHSRD
                     GCAVIELDRIFRRRSDLD"
     gene            complement(890..>1573)
                     /gene="panC"
                     /locus_tag="VGC82_21035"
     CDS             complement(890..>1573)
                     /gene="panC"
                     /locus_tag="VGC82_21035"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY0425816.1"
                     /translation="VTSIFVNPMQFGANEDLSRYPKDEAGDLAALRAEGCALVWLPDV
                     ATMYPAGEATAVTVAGPAERWEGDARPGHFRGVATVCAKLFGQVRPDRAYFGEKDWQQ
                     LQVIKRMVADLLLPLEIVGIETVREPDGLAMSSRNRFLSAQERATAPVLYSVLREAAK
                     VLVGGMAAGDVLAAARASLERQGFAVDYFTLVDGPGLAPIEVAVPGSRLIAAARLGSV
                     RLLDNVQAA"
BASE COUNT          296 a          515 c          463 g          299 t
ORIGIN      
        1 atctgccagg caggcatgga agtgcgaggc aggcatggca ttgcgcacat ctagtcgaga
       61 tcgcttcgtc gccggaagat gcggtccagt tcaatgacgg cacagccgtc gcgtgaatgg
      121 aagctgaccg ggtcaacctg gctgaccaca aacccccggt ccgccatgtg atcgattgcc
      181 tggctaaggg tccacacgtt ttcgtacagg tggatgaccg gcagttccat ctggaggcct
      241 cgcagggagc gtagcgcccg ggcggctccg gcgataacct ggcgttcgaa cccttgcgta
      301 tcgatcttga ggaagcagtt ctcggtggcg aactccgcga agacgtcatc cagcgttgca
      361 acggcaatag tttccgtccg cacgtttgcc gaaactgcgg catggacggt cgcggcgtgc
      421 cgctgtccga gtatcgaact gaagacggat tcggagctga cattgatcgt tgcctggcca
      481 gcccgctccc caagcgcgca gttatgcact gtccaaagtg gatcgcgtct ggcctttgcc
      541 tcgagctgcg cgaacacctg ctttattggt tcgaaggaga cgatgcgccc gcaatatcct
      601 tttttgcgta gcgattccgc gaactggccc atgttggccc caacgtcgaa taccgtggtg
      661 atctcgcgtg acgctaaaaa atccatcagg tccggcataa gcggttcgac gtgcaagccc
      721 actcgccgtg cgatcgatcg gacgagcgat ttccgtttca tacgcagcct gttccttacc
      781 gttgacgaga aagtttccgg ccttcgcgtg acggagttgt cggggacagt tccctagcgg
      841 aacatggcgt gttttcgcga gcgaattatg ccggtgcgcc gaaagtcggt caggccgcct
      901 ggacgttgtc cagcagccgc accgatccca gccgcgcggc ggcgatcagc cgggaccccg
      961 gcaccgctac ctcgattggt gccaaacccg gtccatcgac cagcgtgaag tagtccaccg
     1021 cgaacccctg ccgttccagc gaagcccgtg cggcggccaa gacatcccca gccgccatgc
     1081 cgcccaccag gactttggcg gcttcccgca gcacagaata aaggacaggt gccgtcgccc
     1141 gttcctgcgc cgacaggaac cggtttcgag acgacatcgc caggccgtcc ggctcccgca
     1201 ccgtctcgat cccgacgatc tcgagcggca gcagcaagtc cgcgaccatg cgctttatga
     1261 cttgcaattg ctgccaatcc ttttccccga aataagcccg atccggccgc acctggccga
     1321 acagcttggc gcagaccgtc gcgacaccgc ggaaatggcc gggtcttgca tcaccttccc
     1381 agcgctccgc cgggccggcc acggtgaccg ccgtcgcttc gccggcggga tacatcgttg
     1441 ccacatccgg cagccagacc agtgcgcagc cttccgcccg tagtgctgcc aggtcgccgg
     1501 cttcatcctt tgggtaccgc gacagatcct cattcgcccc gaactgcatt gggttgacga
     1561 aaatcgaggt gac
//
LOCUS       DASWQN010001238         6443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2364804, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001238 DASWQN010000000
VERSION     DASWQN010001238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2364804"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(463..858)
                     /locus_tag="VGC82_21040"
     CDS             complement(463..858)
                     /locus_tag="VGC82_21040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425817.1"
                     /translation="MSDFLGAGGGMHALAHGMFGAVAGDALLGLAHLPSQVGGLFLQQ
                     YGGGFDEATLRPVLVLDIHVHRIIDGRRGDHRVVGGETDLDDVREPYRGGMQAVLDRQ
                     HGALARPVLHRPKDRFVRRCVGLHSKQPA"
     gene            complement(875..1144)
                     /locus_tag="VGC82_21045"
     CDS             complement(875..1144)
                     /locus_tag="VGC82_21045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425818.1"
                     /translation="MLLRFHRGNDAGGFGVKRALTVGVLLVEAAKLRVIGAEPAAQPL
                     NLSGQLLDLRAQLDHGGGNRGLRFGAGLAASGIEPRIRRRMLRLP"
     gene            1127..2257
                     /locus_tag="VGC82_21050"
     CDS             1127..2257
                     /locus_tag="VGC82_21050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425819.1"
                     /translation="MEAEQHAAAERVGTLEALLLKAKAAARGAEKTQIPLNAMTRELE
                     AAGGQLQGVLERIQQTAQQAAVESSEAHEISLAIPPEHPSSPRTMQAMAGATAAAAAL
                     GLILVYLLQLTDSTLRSGEEVRLLTGVPCLALIPEVRKRALGHLRIHDYVVRRPLTAF
                     AEQVRSLRAGISLDIDHPQIITITAARPAEGKSLLTLALGRSAQLGGERVLAIECDLR
                     QGSFQHRLAGDASPGLMEVLRGEAEWRDVLQDDPITGMKFITAGKPGGDVLGLFQSDR
                     MRQVLTEVRDQYDLILLDAPPVEAMTEARIAAALADATLLCVRWRSTPAKTLARTQEM
                     LHDAHAKIIGTVLTRVDHRAHLRSGYADAGVYHRRYTAYFQG"
     gene            2264..3274
                     /gene="galE"
                     /locus_tag="VGC82_21055"
     CDS             2264..3274
                     /gene="galE"
                     /locus_tag="VGC82_21055"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY0425820.1"
                     /translation="MTQRFLVTGGAGYVGSHLVAALLDRGDDVTVLDNLRTGHRAAVP
                     PGARFVAADLADGEQINEVLCAGTWDAVFHFASLSQVGESMRMPMRYLLENAANGIRL
                     IDACVRHGVGRFVLSSTAALFNLDSDAPIPEDAPIDPQSAYGDSKWMIERALRWAGEV
                     HGLRFACLRYFNAAGADPAGRLGEDHRPESHLVPLVIDAALGRRGALDVFGDDYPTPD
                     GTCIRDYVHVSDLASAHLLAVEQLKDGPVVWNLGNGAGHSVRQVIASVELVSGLKVPY
                     RIVGRRAGDASVLVASSIRAQQAGWRPVHCGLDDIVRTAFDWRRSHPDGYGDSDGKSV
                     SR"
     gene            3535..4899
                     /locus_tag="VGC82_21060"
     CDS             3535..4899
                     /locus_tag="VGC82_21060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425821.1"
                     /translation="MQSAIAWLDRHPVLPTIPMLCWPAVWNGYPIVFADTGTYLSQAV
                     HLYAGWDRPVFYSLFMLPLHATITLWPVIVAQALITSWILWLVCRVLTPRITAAGFVG
                     GIAALSVCTWLPWIVSELMPDLFTPLLVLAICLLAWAPERLSRPEAIALVALAAFMIA
                     SQQSSVPLACVLLPVLWLFRRQRGPIVLDEASRHGLSVAIRRGVFVCAPLAVAIAALC
                     SANLAAHGRFALAPFGKVFLLARVIYDGPGMAVLRRDCPAAEWRLCPFIDSFPSSSDE
                     FLWQPDSPLNRAGGPKVVSQDAADIIATALLSDPAGEIRAVVANLVEQLGRFASGDGL
                     NPWPTQVSPWIERDFPASERAAYAAARQQAGPLSVPSLLAGLHRVVAIGGVVACALLL
                     PVALVRRRPSAGFLLIVLVALPVGAAITGGLSAPHDRYQSRLMWLPPFIAAVSLPTLR
                     RRMA"
     gene            4896..6179
                     /locus_tag="VGC82_21065"
     CDS             4896..6179
                     /locus_tag="VGC82_21065"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0425822.1"
                     /translation="MRRDAFWSGLEAIVSAILSIVASFVVARIVGPAELGVGAAAAVH
                     VILWVAVNSLFADALVQRASLDERVMSSAFWASCLVGCLAMVLQAASGWGLAAMFGDS
                     RLLPMCLVLAAPLPLVGAAGVVQGLLTRERAYRRLALRTLIGQLLGTVVGVLAALGGA
                     GAWAFVCQQAVTSTCGALTLLLGRGWKPARHLDPTAIKSLLTVGLPLTASTLVMITRY
                     RLFAVLLGGTAGAVVLGQTHIAFRLVDTVRELTFTALWRLMLPALSQHQQDRQALLFQ
                     VDLWLRRCVMIVGPLCAILAIGLTQVVALVMGPNWTVAGQAALPLVALLAWSACTFPS
                     GVALVALGQARLALYANLGSLVCSTLGVLAWRPTDPWQAVMIWTVSQILVSPYLMWAS
                     ARALRVGIFRPLTGGFTGRTAAGEAGSRLATRSRT"
     gene            complement(6160..>6443)
                     /locus_tag="VGC82_21070"
     CDS             complement(6160..>6443)
                     /locus_tag="VGC82_21070"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY0425823.1"
                     /translation="RQPLDAKAFYGTIVVSTLIGVAINFVGLDPVKALFWAAVINGVV
                     AVPLMVVMMIMAMAPKVMGQFTLPPVLCTMGWLCTAVMTVAVVIMFATW"
BASE COUNT          999 a         2060 c         2175 g         1209 t
ORIGIN      
        1 caaaaaggcc gttgaacgcg gatgctcgac aagcccgccg atagtgtggg ggcggcgccg
       61 gtcccagagc ggggagcgcg gcacctgcct tccgatcaac agcgcatcat ggcgcgggag
      121 agcctgtcgg ggcttgtgtc cgccattcgc cagcggcggc ggaccctgat cgccacgatg
      181 gtgctggtgc cgctatgtgc ctggctgacg ctgcgccaaa tcaccccgct ttataccgcg
      241 acaggctcgc ttatctacga accgagcggc tacaagctgc gcgagctgca gagtatcctg
      301 cgcgaagatc ccacgaccga agggatgatg gccagccagg cggaaatcct ccacagcctg
      361 cacatcgcgc aacgagtggc ggagcggggc aaccttttcg ccaatccgga attcaacgtc
      421 gcccttcggc cgccaggttt cctccaacgt gcgtttctcg ggctacgctg gctgcttgga
      481 atggagaccg acgcaccgcc ggacgaaccg gtctttgggc cggtgcagga ccggtcgcgc
      541 gagcgcacca tgctggcggt ccaggacggc ctgcatgccg ccgcggtacg gttctcgcac
      601 gtcgtcgagg tcagtttcac cgccgacgac ccgatggtcg ccgcggcggc cgtcaataat
      661 gcgatggacg tgtatatcaa ggaccaatac ggggcgaagc gtcgcctcgt cgaagccgcc
      721 accatattgc tgcaaaaaga ggccgccgac ctgcgacggc aggtgcgcca ggccgaggag
      781 cgcatctccg gctaccgcac cgaacatgcc atgagccagg gcatgcatgc cgccaccggc
      841 accgaggaaa tcactcacct gactgaggac ctcgtcaagg cagtcggagc atgcgacggc
      901 gaatgcgcgg ctcgatgccg ctcgcggcaa ggccggcgcc gaagcgcagg ccgcggttgc
      961 cccctccgtg gtccaactgc gcgcgcaggt cgagcaattg tccggacaga ttcaagggct
     1021 gcgcagccgg ctcggcaccg atcacccgga gtttcgcagc ctcaaccagc aatacgccga
     1081 cggtcagcgc gcgcttcacg ccgaaaccgc ccgcgtcgtt gccgcgatgg aagcggagca
     1141 acatgccgcc gcggaacggg tcggaacgct ggaagcactt ctgctcaagg ccaaggcggc
     1201 ggcgcgcggc gcggagaaga cgcaaattcc gctcaacgcc atgacccgcg agctggaagc
     1261 ggccggagga caactgcaag gggtgctgga gcgtatccag cagacggcac aacaggccgc
     1321 cgttgaatct tccgaagcgc atgaaatatc gctggcgatc ccgccggaac acccgagcag
     1381 cccgaggacg atgcaggcca tggcgggagc gacggccgcg gcggcggctc tggggctgat
     1441 cctggtttac ctgctgcaac tgacggacag caccctgcgc agcggcgagg aagtccggct
     1501 tctgaccggc gtgccctgcc tcgcgttgat cccggaagtg cgcaaacgcg cgctggggca
     1561 cctgaggatc cacgactatg tcgtccggcg cccgctgacg gccttcgccg aacaggtgcg
     1621 ttcgctgcga gccggcatat ccctcgatat cgaccacccc cagatcatca ccatcaccgc
     1681 cgcccgcccg gcggaaggca agtcgctgtt gacgctggcg cttgggcgtt cggcgcaact
     1741 ggggggcgaa cgggtcctgg ccatcgaatg cgacctgcgg caggggtcct tccaacatcg
     1801 cctggccggc gatgcttcac cgggcctgat ggaagtgctg cgcggcgagg cggaatggcg
     1861 cgatgtcctg caggacgatc cgatcaccgg catgaaattc atcaccgcgg ggaagcccgg
     1921 cggggacgtg ctgggcttgt tccagtcgga caggatgcgc caggtcctga cggaagtgcg
     1981 cgatcaatac gacctgatcc tgctggacgc gccaccggtc gaggcgatga ccgaggccag
     2041 gatcgccgcc gccctggcgg atgccacgct gttgtgcgtg aggtggcgct cgacgccggc
     2101 caagaccttg gcccgcacgc aggagatgct gcacgacgcg cacgccaaaa ttatcggcac
     2161 cgtgctgaca agagtcgatc accgtgcgca tcttcgttcc ggatacgccg atgcgggggt
     2221 atatcatcgc cgatacacag cgtatttcca ggggtaggtg ttgatgacgc agcgttttct
     2281 ggtgaccggc ggcgccggct atgtcggcag ccacctggtt gcggccctgc tggaccgagg
     2341 cgacgatgtc accgtactgg acaacctgcg gaccggccat cgtgctgcgg tgccgccggg
     2401 tgcgcggttc gtcgcggcgg atctcgcgga cggcgaacag atcaatgaag tgctgtgcgc
     2461 tggcacctgg gatgcggtct tccatttcgc ctcgctgtcg caggtgggcg agagcatgcg
     2521 catgccgatg cgctacctgc tggagaacgc cgccaacggc atcaggctga tcgacgcctg
     2581 cgtccgccat ggggtgggac ggttcgtgct gtcctccacc gctgcgctgt tcaacctgga
     2641 tagtgacgcg ccgatccccg aggatgcgcc gatcgacccg caatcggcct atggcgacag
     2701 caaatggatg atcgagcgcg cgttgcgctg ggcgggcgag gtgcatggac tgcgcttcgc
     2761 ttgcctgcgc tacttcaatg ccgccggtgc cgatcccgcc gggcgcctcg gtgaggatca
     2821 tcggccggaa tcgcatctcg tcccactggt gatcgatgcc gcattggggc gccgcggcgc
     2881 cttggatgtg ttcggcgacg actatccgac gcccgatggc acctgcatcc gcgactacgt
     2941 gcatgtgtcc gacctggctt cggcgcatct gctggctgtg gagcagttga aggacgggcc
     3001 ggtggtatgg aacctcggca atggtgccgg ccattcggtc cgccaggtga ttgcctcggt
     3061 ggagctggtg tcgggactga aggtgcctta tcggatcgtc gggcgccggg ccggcgatgc
     3121 ctcggtgctg gtggcgtcct cgatacgagc gcaacaggcg ggctggcggc cggtacactg
     3181 cggcctggac gacatcgtca ggaccgcgtt cgactggcgc aggtcgcacc cggatggtta
     3241 tggcgatagt gatggcaaaa gcgtttcacg ctgacgggaa aaggtgccgg ccagctcgcg
     3301 gcatggcgcc tatgtgtcat ggccgcgctt ggcgcgggca cccacgactt cctttgcagc
     3361 cgcaagagaa cccgctgttt ccggtcaccg caaaaccgct ctaaccagca atttcacgca
     3421 gtgccaggcg cgagtgccgc tgcataggga tgtccgacga aaattacgta agcgattagc
     3481 aattgagtaa ccttcgtccg ctatgaagac cgctttcggc gggagggcga attattgcaa
     3541 agcgccatcg catggctcga tcgccacccg gttctgccga cgatcccgat gctgtgttgg
     3601 ccggcggtgt ggaatggcta tccgatcgtg ttcgccgata ccgggactta cctgtcgcag
     3661 gcggtccatc tttatgccgg ctgggaccgg ccggtgttct actcgctgtt catgctgccg
     3721 ctgcacgcaa cgatcaccct ctggccggtg atcgtcgcgc aggcgctgat cacgagctgg
     3781 attttgtggc tcgtttgcag ggtgttgacg cctcggataa cggctgccgg gttcgtcggc
     3841 ggcatcgcgg ccctgtccgt ctgtacctgg ctgccatgga tcgtcagcga actgatgccc
     3901 gatttgttta cgcctctcct ggtgttggca atctgcttgc tggcctgggc accggagcgg
     3961 ctgtcccgtc cggaggctat agcgctggtg gcgttggcgg cattcatgat cgcaagccag
     4021 caatcaagcg tgccattggc gtgcgtattg ttgccggtgc tgtggttatt tcgccgccag
     4081 cgtgggccga tcgttttgga tgaagcctcc cgccacggtc tttccgtcgc catccgtcga
     4141 ggggtgtttg tttgcgctcc gcttgctgtg gcgattgccg cactgtgttc cgctaatctc
     4201 gccgcgcatg ggcgcttcgc ccttgctcca tttggcaagg tatttctgct tgcccgggtg
     4261 atctacgacg gtcctggcat ggctgtccta cgtcgcgact gtccggcggc ggaatggcgt
     4321 ctgtgccctt tcatcgacag ctttccgtcg tcatcggacg aatttctttg gcaaccggat
     4381 agtccgttga accgcgccgg tggtccgaaa gtcgtctcac aggacgccgc ggacatcatc
     4441 gccaccgcgc tgctctctga tcccgctggc gaaatccgag cggtcgtggc caatctggtc
     4501 gaacaactcg ggcggttcgc cagtggcgat ggattgaatc cttggcccac ccaggtttca
     4561 ccgtggatcg aacgtgactt cccggcatcg gaacgcgctg cctacgccgc cgcgcggcag
     4621 caggcgggac cgctgtcggt gccttcgctg ctggcgggcc tgcatcgcgt agtcgcgatc
     4681 ggcggtgtcg ttgcttgcgc gttgctgctg ccggtcgccc tggttcggcg ccggcccagt
     4741 gcgggcttcc tgctcattgt ccttgtcgcc ctgccggtcg gcgcggcgat caccggcggc
     4801 ctgtcggcgc cgcatgaccg ctatcaaagt cgcctcatgt ggctgccgcc cttcatcgca
     4861 gccgtctcgt tgccaacgct tcgccggcgg atggcatgag gcgcgacgca ttctggtcgg
     4921 ggctggaggc aatcgtctcc gccatcctgt cgatcgtcgc ctcgttcgtg gtcgcccgca
     4981 tcgtggggcc cgcggagttg ggtgtagggg cggcggcggc agttcatgtc atcctgtggg
     5041 tggcggtgaa ttccctgttc gccgatgcgc tggtgcagcg cgccagcctc gacgagcgcg
     5101 taatgtccag cgcgttctgg gcctcgtgcc tggtgggatg cctcgcaatg gtattgcagg
     5161 cagcgtccgg ctgggggctt gccgcgatgt tcggcgacag ccggctgttg ccgatgtgcc
     5221 tggtgcttgc ggcaccgctt ccgctggtcg gcgccgccgg cgtggtccag gggttgctga
     5281 cgcgcgaacg cgcttatcgc cggctggcat tgcgcacgct gatcgggcag ctcctgggaa
     5341 cggtcgtcgg agtcctggcg gcattgggcg gagccggtgc ctgggcgttc gtttgccagc
     5401 aggccgtgac ctcaacctgc ggagccctga cgctgctgct ggggcgcggc tggaagccgg
     5461 cacgccacct tgacccgacc gcaatcaagt cgttgctgac ggtgggcctc ccgttgaccg
     5521 cctccaccct ggtcatgatc acgcgctacc gcctgtttgc cgtattgctc ggggggacgg
     5581 ccggcgctgt cgtcctgggc cagacgcata tcgcgttccg gctggtcgat accgtgcggg
     5641 agctgacatt caccgcgctc tggcggctga tgctgccggc cctgtcgcag catcaacagg
     5701 atcgtcaggc gctgcttttc caggtcgatc tgtggctgcg gcggtgcgtg atgatcgtcg
     5761 gaccgctttg cgcgatcctc gccatcgggc tgacccaggt cgtggcattg gtcatggggc
     5821 cgaactggac cgtggccggt caagccgctt tgccgctggt cgcgctgctc gcatggtcgg
     5881 cctgcacctt cccttccggc gtcgcgcttg tcgcgcttgg gcaggcccgg cttgccctgt
     5941 acgccaacct ggggagcctg gtttgcagca cactgggcgt gctggcgtgg cggccgacgg
     6001 acccatggca ggcggtcatg atctggacgg tcagccagat tctggtcagc ccctacctga
     6061 tgtgggcgag tgcccgagcg ttgcgggtcg gcatcttccg cccgttgacc ggcgggttca
     6121 ccggccgaac ggcagcgggc gaggcagggt ctcggttggc taccaggtcg cgaacatgat
     6181 gacgacggcg accgtcatga cggcagtgca cagccatccc atcgtgcaca gcacgggcgg
     6241 gagcgtgaac tggcccatca ccttgggtgc catcgccatg atcatcatca cgaccatcag
     6301 cggcaccgcg accacaccgt tgatcacggc ggcccagaac agcgccttca ccgggtccag
     6361 gccgacgaag ttgatcgcga ccccgatcag ggtggacacg acgatcgtgc cgtagaatgc
     6421 ctttgcatcc agcggctggc ggt
//
LOCUS       DASWQN010001239         2539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2630404, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001239 DASWQN010000000
VERSION     DASWQN010001239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2630404"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VGC82_21075"
     CDS             complement(<1..303)
                     /locus_tag="VGC82_21075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425824.1"
                     /translation="MLKEGSDWIQLHPGLHPKQLMKITALNPVVRLSDVVVGIPPMSV
                     VLGICSLTRNSGWACGMGFQQIPTGEPSESGVRYGFLGFARARRFAGATGWTSPRSA"
     gene            complement(325..398)
                     /locus_tag="VGC82_21080"
     tRNA            complement(325..398)
                     /locus_tag="VGC82_21080"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Gly,seq:ccc)
     gene            complement(507..2210)
                     /locus_tag="VGC82_21085"
     CDS             complement(507..2210)
                     /locus_tag="VGC82_21085"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0425825.1"
                     /translation="MAYDLLIKDGLVVDGSGMPAFRGDVGVKDGKIVEIGKLDGPATR
                     LVHADGRAVAPGFIDNHAHYDGQVTWDPLCTFSPQHGATSVIFGNCSLALAPVRRGGK
                     HRTAEFLSYVEAIPMEVLSTIDMDWETIPQYMDKLDKSLGVNTGTLIGHTPVRYYVMG
                     DDCQKREATPAEIKAMQDVVREGMTAGALGLSLSRQKGHYDPQGVPIPALWASDEEIF
                     ALGDVLRELSTGTIQVGGGVDQELKDGMMARLSDATGRMVVYNSLSQTVRSPDKWKQH
                     MARVDETVAQGIRAYPMCSPNRVTQDFTMRNCQVFRGLPTWHPILLASDDEKMRAYAD
                     PAIRDKLHAEAVERSVPVNVVGFSKTWWDYMWVNEPALEKNRSLQFKSIGEIAQMQGK
                     RVIDAFLDLVVEEKLETRFLQAENNIDDEAMTKILTYPNALVGLGDGGAHVQFHGGYG
                     YTTRLLGEWVRERKVLTIEQAVRKLTFESASAFGIHDRGMLRPGLAADVVIFDPATVK
                     AGKETVVHDFPGGGWRIKEEAEGVHYTIVNGQVLLEDGEHTGALPGRVLRNSWYHAHR
                     N"
BASE COUNT          444 a          815 c          773 g          507 t
ORIGIN      
        1 tgccgaacgg ggtgaggtcc agccggtcgc gccagcgaaa cggcgcgccc gcgcaaagcc
       61 caagaagccg tagcgaacgc cagattccga cggctcgccc gttgggatct gttggaaacc
      121 catcccacaa gcccagccag aatttctggt caggctgcaa atgcctagta cgacgctcat
      181 gggtgggatg ccgaccacga catcgctgag ccgaaccacc gggttcaaag cagttatttt
      241 catcaattgc ttagggtgaa ggccaggatg aagctgaatc cagtccgaac cctctttcag
      301 catcgtgcca aaagcaagga ttaatggagc gggtgaaggg aatcgaaccc tcgtgtgcag
      361 cttgggaagc tgccgttcta ccattgaact acacccgcgc ccggtgctgc ttttaggcgg
      421 ttcttgccgg ggatgcaatt caggccggcg tcgaaccccc gagtcaaagg ggctcgatcg
      481 cacaacctgg taatccaatc actgcgtcag ttgcggtggg cgtggtacca actgttccgc
      541 agcacgcgcc cgggcagcgc cccggtgtgc tcgccgtcct ccagcagcac ctggccgttg
      601 acgatggtgt agtgcacgcc ctcggcctct tccttgatcc gccagccgcc gccggggaag
      661 tcatgcacca cggtttcctt gcccgccttc accgttgccg ggtcgaagat caccacgtcg
      721 gccgccaggc ctggccgcag catgccgcgg tcgtggatgc cgaaggccga ggccgactcg
      781 aacgtcagtt tgcgcaccgc ctgttcgatc gtcaggacct tccgctcgcg cacccactcg
      841 cccagcaaac gggtggtgta gccgtagccg ccgtggaact gcacatgcgc gcctccgtca
      901 cccagtccga ccagtgcatt cggataggtc aggatcttcg tcatggcctc gtcgtcgatg
      961 ttgttctcgg cttgcaggaa gcgagtctcc agcttttcct cgaccaccag atccaggaac
     1021 gcgtcgatca cccgcttgcc ctgcatctgg gcgatctcgc cgatgctttt gaactgcaag
     1081 gaccggttct tctccagtgc cggctcgttt acccacatgt agtcccacca ggtcttggag
     1141 aagccgacga cgttgaccgg caccgaacgc tccaccgcct cggcatgcag cttgtcgcgg
     1201 atggccggat cggcataggc gcgcatcttc tcgtcgtctg aggcgagcag gatcggatgc
     1261 caggtcggca acccccggaa cacctggcag ttgcgcatcg tgaagtcctg cgtcacccgg
     1321 ttcgggctgc acatcggata ggctcggata ccctgggcga cggtttcatc gacccgcgcc
     1381 atgtgctgct tccatttgtc gggtgaccgc acggtctggc tcaggctgtt atagacgacc
     1441 atccgtccgg tcgcgtcgga cagccgagcc atcatcccgt ccttcagttc ctgatccacg
     1501 ccgccaccga cctggatcgt gccggtggac agctcccgca acacgtcgcc gagcgcgaag
     1561 atttcctcgt cgctggccca cagcgccggg atcggcacgc cctgtgggtc gtagtggccc
     1621 ttctgtcggg acaacgacaa acccagcgcg ccggcggtca tgccctcacg gacgacatcc
     1681 tgcatggcct tgatttcggc aggcgtggcc tcccgcttct ggcaatcgtc gcccatgacg
     1741 tagtagcgca ccggggtgtg gccaatcagg gtgccggtgt tgacgcccag gctcttgtcg
     1801 agcttgtcca tgtattgcgg gatggtttcc cagtccatgt cgatggtgct cagcacctcc
     1861 atcgggatcg cttcgacgta cgaaaggaat tccgcggtgc ggtgtttgcc gccgcgccgc
     1921 accggcgcga gtgccagcga gcagttgccg aagatcaccg acgtggcgcc atgttgcggc
     1981 gagaaggtgc agagcgggtc ccacgttacc tgcccgtcat aatgggcatg attgtcgatg
     2041 aacccgggcg ccaccgcgcg tccgtccgcg tggaccaggc gtgtcgccgg accatcaagc
     2101 ttgccgattt cgacgatttt gccgtctttg accccgacgt cgccgcggaa cgcgggcatt
     2161 ccggagccgt caacaaccag gccgtccttg atcaagagat cgtatgccat tggttagccc
     2221 tcccatcgca gggccgccca tcgcgaaccc tgtcgcttag gtcggagttt ttgacctcgt
     2281 ttgaagaatg tcaatcggcg cttgagcggg ggcagtagct gggtgtcccg gcctcatgcg
     2341 cttcaagaac gttcgttgtc cgtcattccg cccgtccaag ggcaggagtc cggcgtgggt
     2401 ttggctgggc cttgggttta gctgggcctt gggtttagct gggcgtagat tgtaaagggg
     2461 caaaaccccg ggcgcgcaga tgcagcatga tgcaacctca acggaccgag tgacggaaat
     2521 ccgccatcgt tgtgctgat
//
LOCUS       DASWQN010001240         4083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2630411, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001240 DASWQN010000000
VERSION     DASWQN010001240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2630411"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VGC82_21090"
     CDS             complement(<1..746)
                     /locus_tag="VGC82_21090"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0425826.1"
                     /translation="MTVLTRRTVLSGAATLLAAPAIGAGKYSPGASDTEIKIGQTMPY
                     SGNASAYGQNGRAQAAYFRMINEQGGINGRKINLISLDDGYSPPKTVELTRQLVERDQ
                     VLFLFSPLGTQCNTAIQKYLNQKKVPQLFVASGASKWGDPAHFPWTMGWQPDYRTEGM
                     IYAQHIKTTVKNPKIAILRQNDDMGTDYVGGFREQIAAFKDTVGPDAIVADATYEVTD
                     PTVDSQILQLKNSGANVFYNITTPKFAAQA"
     gene            complement(743..1783)
                     /locus_tag="VGC82_21095"
     CDS             complement(743..1783)
                     /locus_tag="VGC82_21095"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0425827.1"
                     /translation="MAASWATKRGVGTGAILFWIFMALLPLSGFVLQDSSFDYYMFMG
                     QSMLIYAIAVLGLNLLIGFNGQISLGHSAFFALGAYTAAILMNFGGWPYWATLPVAAA
                     LCFLAGLAFGFPALKLEGHYLALATFTLAIAMPQLLKYKAIAGWTGGVGGVLLEKPDP
                     PEWTGLSPDQWLYYVCLIVAILLFWLVFNLLRGRSGRALMAIRDHAMAASTMGIGLAR
                     YKTIAFGISAGITGIAGALSAITVAYVAPDSFTLQLSISFLVGLVVGGIASLPGCLVG
                     GVFIVLIQNSAQSISDWMKATFALRYDVPAWAIYGVILLLLMYLLPAGIAGGFAQIRS
                     WWTTRGRREARQ"
     gene            complement(1787..2659)
                     /locus_tag="VGC82_21100"
     CDS             complement(1787..2659)
                     /locus_tag="VGC82_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0425828.1"
                     /translation="MEFFVQQIASGLTSGAIYACVALGLVMIYVSTDHINFAQGEMAM
                     FATYLSWAMINAGVPFWVAFGLTIAIAFSGGLFIQRVILKPLENAPVLALVVSFIALL
                     SIFNAMAGMIWSYTIKQFPSPFPNRDLFGNGLVSIQQLGVVGVTLIMLGFLTVFFRYT
                     TLGLAMRAAAQNPVSARLMGVRVSWMLALGWGLAAAIGAVAGMLVAPIVFLDPNMMTG
                     ILVYGFASALVGGIGNPMGAVAGGFIVGVLENLVGSYVIGNELKLTFALVVVIGVLLL
                     KPAGLFGRVIVRRV"
     gene            complement(2669..3382)
                     /locus_tag="VGC82_21105"
     CDS             complement(2669..3382)
                     /locus_tag="VGC82_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425829.1"
                     /translation="MVAVLEAKGLSAWYGPIRALYGLDLVVEQGGVTALLGANGAGKT
                     TTLRAICNMVRRTGTTSFLGSDISRGSTEDIVRMGVAHVPEGRGTFVALTVEENLRLA
                     FHTRRDRAAALRDMDLVFGYFPILKERLHQQAGTLSGGEQQMLAIGRALMLGPKLMLL
                     DEPSFGLAPLVVREIFRILRRINEHENVSMLIVEQNAALALDLADTVYLLETGKVVTS
                     GPSAVIQDDEALRKAYLGY"
     gene            complement(3382..>4083)
                     /locus_tag="VGC82_21110"
     CDS             complement(3382..>4083)
                     /locus_tag="VGC82_21110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425830.1"
                     /translation="VTFDVMPGQIAGLIGPNGAGKTTLFNCLSRLYQPSAGEIIFEGQ
                     SVLSVPRHKIPTIGIGRTFQNVALFDRLTVLENVMVGRHSRSSAGFVENALRLPSVGR
                     EEALVREKARDLIEFMNLTAFTDRVAAGLPFPIRKRVELARALAAEPKLLLLDEPAAG
                     LNHDEVHTLEGQIRRVRDVQGVTVLLVEHHMSLVMSVSDKVVVVNFGRKIADGRPDEV
                     RQNPDVIRAYLGTAA"
BASE COUNT          837 a         1325 c         1232 g          689 t
ORIGIN      
        1 atggcctgcg cggcgaattt cggcgtcgtg atgttgtaga aaacattcgc gccggagttc
       61 ttcaactgca ggatctgtga atcgaccgtc gggtcggtga cctcgtaggt cgcatcggcg
      121 acgatcgcgt cgggaccgac cgtgtccttg aaagcggcga tctgctcgcg aaagccaccg
      181 acatagtcgg ttcccatgtc gtcgttctgc cgcaggatgg cgatcttcgg gttcttcacc
      241 gtcgtcttga tgtgctgggc gtagatcatg ccctcggtcc ggtaatcggg ctgccagccc
      301 atggtccagg ggaaatgcgc gggatcgccc catttcgacg cgccggaggc gacgaaaagc
      361 tgcggcacct tcttctggtt caggtatttc tggatcgccg tgttgcactg ggtgcccagc
      421 ggcgagaaca ggaacagcac ctggtcgcgc tcgacaagct ggcgcgtcag ctccaccgtc
      481 ttgggcggac tatagccgtc gtccaacgag attagattga tcttgcgccc gttgatcccg
      541 ccctgttcgt tgatcatccg gaaatacgcc gcctgcgctc ggccgttctg accataggcc
      601 gaagcgttgc cgctataggg catcgtttga ccgatcttga tctcggtgtc cgatgcgcca
      661 gggctatact tgccggcacc gatcgcaggt gccgcgagca acgtcgccgc tccggacaga
      721 acggttcgcc gcgtcaatac tgtcattgtc ttgcctccct tcttcccctt gtcgtccacc
      781 acgaccgaat ctgcgcgaac ccacccgcga tgccagccgg gagcagatac atcagcaaca
      841 gcagaataac gccatagatc gcccaggccg gcacgtcata ccgcagggcg aaggtcgcct
      901 tcatccagtc ggagatcgac tgcgccgagt tttgtatcag cacgatgaac acgccgccca
      961 ccaggcaccc cggcagcgaa gcgatgccac ccaccacgag tccaaccagg aacgagatcg
     1021 aaagttgcag cgtgaaactg tcgggcgcga catacgccac ggtgatcgcc gacagcgcgc
     1081 cggcgatgcc ggtgatgccg gccgagatgc cgaatgcgat cgtcttgtag cgcgcgagac
     1141 caatccccat cgtgctggct gccatcgcgt ggtcgcggat ggccatcagg gcccgcccgc
     1201 tgcgtccgcg aagtagattg aacacaagcc agaacagcaa aatcgcgacg atcaggcaaa
     1261 cgtagtacag ccattggtca ggcgagagtc ccgtccattc cggcggatcg ggcttttcga
     1321 gcaagacgcc cccgacgcct ccggtccagc cggcgatcgc tttgtatttc agcaactgcg
     1381 gcatggcgat tgccagcgtg aacgtggcca gcgccaaata atgcccctcc agcttcaggg
     1441 ccgggaatcc gaatgccaga ccggcgagga aacacagcgc ggctgccacc ggcagcgtcg
     1501 cccagtaggg ccatccgcca aaattcatca ggatcgcggc ggtataggcg cccagcgcga
     1561 agaatgcgct gtggccaagg gagatttgcc cgttgaagcc aatcagcagg ttcaatccca
     1621 gcacggcgat cgcgtagatc agcatcgact ggcccatgaa catgtaatag tcgaaactgc
     1681 tgtcctgaag aacaaatccg ctcagcggca gcaacgccat gaagatccag aacaggatgg
     1741 cgcctgtccc aacgccgcgt ttggttgccc agctcgcggc catcgctcag acccggcgca
     1801 cgatgacgcg gccgaacagg ccggcgggct tgagcaacaa cacaccgatc acgacgacca
     1861 gcgcgaaggt cagcttcaac tcgttgccga tgacgtagct gccgaccagg ttctccagca
     1921 cgccgacgat gaagccgccg gcgaccgcgc ccatcggatt gccgatgcca cccaccagcg
     1981 cgctggcgaa cccgtacacc aggatgccgg tcatcatatt cggatcaagg aacacgatcg
     2041 gcgccaccag catacccgcg accgccccga tcgccgccgc gaggccccag cccagggcca
     2101 gcatccagga aacccgcacg cccattagac gggccgatac cgggttctgc gccgccgcgc
     2161 gcatcgcgag gcccaaggtc gtgtagcgaa agaacaccgt aagaaagccc agcatgatca
     2221 aggtcacgcc gacaacgcca agctgctgga tgctgaccag cccgttgccg aacagatccc
     2281 gatttggaaa ggggcttggg aactgcttga tcgtgtagct ccagatcatg cccgccattg
     2341 cattgaagat cgagagcagg gcgatgaacg acaccaccag cgccagcacc ggcgcatttt
     2401 caagcggctt gagaatgacg cgctgaatga acaacccgcc ggaaaacgcg atggcgatcg
     2461 tcaggccgaa ggcgacccaa aaggggacac cggcgttgat catcgcccac gacagatagg
     2521 tggcgaacat cgccatctcg ccctgggcga aattgatgtg gtcggtggaa acgtagatca
     2581 tcaccagacc gagcgcgaca caggcataga tggcgccgct ggtcagtccc gacgcgatct
     2641 gctggacgaa gaattccatg cgaaatcctc aatatcccaa atacgccttg cgcagcgcct
     2701 cgtcatcctg aatgacggcg gacgggccgg acgtcacgac cttgccggtt tccagcaaat
     2761 agaccgtatc ggccaggtcc agcgccagcg cggcgttctg ctcgacaatc agcatggaca
     2821 cgttctcatg ctcgttgatc cgtcggagga tgcgaaagat ttcccgcacc acgagcgggg
     2881 ccaggccaaa ggatggctcg tccagcaaca tgagcttggg accgagcatc agcgcgcggc
     2941 cgatggcgag catttgctgt tcgccgccgg acagggtgcc ggcctgttgg tgcagccgct
     3001 ccttcaggat cggaaaatag ccgaacacca gatccatatc acgcagcgcg gcggcccggt
     3061 cgcgcctggt atggaacgcc agccgaaggt tctcctccac cgtcagcgcg acaaacgtgc
     3121 cgcgaccttc cgggacatgc gccacgccca tgcgcacgat gtcctcggtg ctgccgcggc
     3181 tgatatcgga ccccagaaac gacgtggtgc ccgtccggcg caccatgttg cagatcgccc
     3241 gcagcgtggt ggttttgccc gctccgttgg cgccgagcag ggcggtgacg ccgccttgct
     3301 cgacgacgag gtcgagaccg tacaaggccc gtatcggccc ataccacgcg ctgaggccct
     3361 ttgcctccag cacagcaacc atcaggcggc ggtgccaaga taggcgcgga tgacgtccgg
     3421 gttctgccgg acctcatcgg ggcggccgtc ggcgatcttg cggccgaaat tgaccaccac
     3481 caccttgtcc gacacggaca tcaccaggct catgtgatgc tcgaccaaca gcacggtcac
     3541 gccttggaca tcccgcaccc ggcggatctg gccttccagc gtgtgaacct cgtcgtgatt
     3601 gaggcccgct gctggctcgt cgagcagcag cagcttgggt tcggcggcaa gggcccgggc
     3661 gagttccacc cgcttgcgga tcgggaacgg cagcccggct gccacccggt cggtaaaggc
     3721 cgtgagattc atgaattcga tcaggtccct tgccttctcc cgcaccaagg cttcttcccg
     3781 gccgacggag ggcaagcgca gcgcattttc gacgaagccg gcgctgctgc ggctgtggcg
     3841 gccgaccatc acgttctcca gcacggtcag ccgatcgaac aacgcgacgt tctggaaggt
     3901 gcgcccaatg ccgatggtcg ggatcttgtg ccgcggcacc gacagaaccg actggccttc
     3961 aaagatgatt tccccggcgc tgggctgata aagccggctg aggcagttga aaagcgtggt
     4021 tttcccggca ccgttagggc cgatcaggcc ggcgatctgg cccggcatga cgtcaaaggt
     4081 gac
//
LOCUS       DASWQN010001241         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_650103, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001241 DASWQN010000000
VERSION     DASWQN010001241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650103"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VGC82_21115"
     CDS             <1..208
                     /locus_tag="VGC82_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY0425831.1"
                     /translation="IAELALEYDVHPNQIHNWKKILLDGAAVVFEAGSVREDKAGEAQ
                     VGELYRQIGQLKVEKDFLAKRLGK"
     gene            229..1011
                     /locus_tag="VGC82_21120"
     CDS             229..1011
                     /locus_tag="VGC82_21120"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY0425832.1"
                     /translation="MIDRAHPTLPVTHQCRILNVSRATVYRRPAAVSDADLELMALID
                     RQYLARPFYGSRRMAAWLNTQGHAVNRKRVQRLMRLMGLVAICQRPNTSKAASEQVKY
                     PYLLGGMTIDRVNQVWCSDITYIPMPRGFLYLVVIMDWHSRAVLSWRLSNTLHADFCV
                     EALEEALRQFGRPDIFNTDQGSQFTSDDFIGVLKRDQIKISMDGKGRCMDNVFVERLW
                     RSLKYEEIYLHAYASVSEAKSGIGAWLHFYKCVSYCPTWLCH"
     gene            complement(1012..>2279)
                     /locus_tag="VGC82_21125"
     CDS             complement(1012..>2279)
                     /locus_tag="VGC82_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0425833.1"
                     /translation="GPRRGIRRKPREDWLALHPGAHEGYVDWERSETIRAMIRDNNVS
                     EGGRGAAKRGDALLAGLLRCRRCGRKLTIRYTGNHHDVLRYACNRGWLDSGEPQCIAF
                     GGLTVDAAVGAQVLQAVDPGAVEAALQEYRQEARRRDDVRDALRRDLEAARYAADRAF
                     RQYDAADPLNRHVAAELEVRWNRTLERVAALENRIEAHDRALPARSPATADEFVTLAD
                     DLKVVWQDPSADVRLKKRIVRTLLQEVVADVDDEAGAIVLVLHWAGGIHTELRLPRRR
                     RGQCRGTSAEIVEAVRVLVRIAGDDLIAGLLNRNGLVTGRGNRWTRERVAALRSHNKI
                     PVYRPETADADGWMNLTRAAAFLGVSAKTLRLAAENGDIEAEHPLADGPWLFRQVHLE
                     DDAGRRIANQARQRRRSPAGPVVEQQTLF"
BASE COUNT          382 a          735 c          754 g          408 t
ORIGIN      
        1 gatcgccgag ctggcgctgg agtacgatgt gcatccgaac cagatccaca actggaagaa
       61 gatcctgctg gacggcgccg cggtcgtctt tgaggcgggg agcgtgcgcg aagacaaggc
      121 cggtgaggcg caggtcggcg aactgtaccg gcagatcggc cagctgaagg ttgaaaagga
      181 ttttttagcc aagaggctcg gcaagtaacc cgggccgagc gccgcgcgat gatcgaccgc
      241 gcgcatccca ccctgccggt gacccatcag tgccggatcc tgaacgtcag ccgggccacg
      301 gtctatcgcc gcccggcggc ggtcagcgac gcggacctgg agctgatggc actgattgac
      361 cggcagtacc tggcccggcc gttctacggc tcgcggcgga tggcggcgtg gctgaacacg
      421 caaggccacg cggtgaaccg caagcgggtg cagcggctga tgcgcctgat ggggctggtg
      481 gcgatctgcc agcgccccaa taccagcaag gctgcgtcgg agcaggtcaa gtatccctac
      541 ctgcttggag gcatgacgat cgaccgtgtc aatcaggtct ggtgctcgga tattacgtac
      601 attccgatgc cccgcggctt tctgtacttg gtggtgatca tggattggca cagccgtgcc
      661 gttctgtcct ggcgcttgtc caacaccttg catgccgact tctgtgtcga ggccctggag
      721 gaggcgttgc gccagttcgg acggccggac atcttcaaca ccgaccaagg cagccagttc
      781 accagcgatg actttatcgg tgttttgaag agggaccaga tcaagatcag catggacggc
      841 aagggtcgtt gcatggataa tgtcttcgtg gagcggctct ggagaagttt gaagtacgaa
      901 gagatatatc tgcacgctta tgcttctgtt tccgaagcga aatccgggat cggagcatgg
      961 ctgcacttct acaagtgcgt ttcatactgc cctacctggc tatgtcattg atcagaatag
     1021 cgtttgctgt tccactactg gtcccgcggg ggatctgcgg cgctgacggg cctgattggc
     1081 gatccgtcgc ccggcgtcgt cctcaaggtg gacctgccgg aacagccatg gcccatcggc
     1141 gagcgggtgc tcagcctcga tgtcaccgtt ctcggcggcc aggcgcagcg tcttggcgct
     1201 gacgccgagg aatgccgcgg cccgggtcag gttcatccag ccgtctgcgt cggcagtctc
     1261 cgggcggtaa accgggatct tattgtggga ccggagggcg gcaacgcgct cccgtgtcca
     1321 gcggtttccc cggccggtga cgaggccgtt gcgattgagc agtccggcga tcaggtcgtc
     1381 accggcaatc cgcaccagca cgcgcacggc ttcgacgatc tcggcggagg ttccccggca
     1441 ttgcccgcgg cgccggcgcg gcaggcgcag ttcggtgtgg ataccgccgg cccaatggag
     1501 cacgaggaca atcgcgcctg cctcgtcgtc gacgtccgcg acgacctcct gaagcagggt
     1561 ccgcacgatc cgcttcttca ggcggacgtc ggccgacggg tcttgccaca cgaccttcag
     1621 gtcgtcagca agcgttacga actcgtcagc tgtcgccggc gaccgcgccg gcaaggcccg
     1681 atcatgcgcc tcgatccggt tttccagggc ggcgacgcgc tcgagagtgc ggttccagcg
     1741 cacctccaac tccgccgcaa catgacggtt cagcggatcg gcggcgtcat attgccggaa
     1801 ggcacggtcg gctgcgtacc gggcggcctc gagatcgcgc cggagggcat cccggacgtc
     1861 gtcacggcgc cgggcttcct gccggtactc ctgcaaggcg gcctccaccg ctcccgggtc
     1921 gacggcctgc agaacctgtg ccccgacggc ggcgtccacc gtcagcccgc caaaggcgat
     1981 gcactgcggt tcgccgctgt ccagccagcc acggttgcag gcgtagcgca ggacgtcatg
     2041 atggttcccg gtgtagcgga tcgtcagttt acgaccacag cgccggcagc gcaggaggcc
     2101 ggcgaggagc gcgtcccccc gtttggccgc accgcgtccc ccctcactca cgttgttgtc
     2161 ccggatcatc gcgcggatcg tctcggaccg ttcccagtcg acatagccct cgtgcgcacc
     2221 gggatgcaag gccaaccagt cctcccgcgg cttgcgccgg atgccccggc gcggacccg
//
LOCUS       DASWQN010001242         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_517567, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001242 DASWQN010000000
VERSION     DASWQN010001242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517567"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VGC82_21130"
     CDS             complement(<1..425)
                     /locus_tag="VGC82_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEY0425834.1"
                     /translation="MAPSSLAPNRKRRALRWLLRWGFIAAIWLTLAAAVMVLWFCRDI
                     PRPESALDAVRRPSLTLQDRAGRIIATFGDLVGEPLRLKDFSPSLPAAVVAVEDRRFY
                     HHFGLDPIGLARAAWVNLNAGRVVQGGSTLTQQVAKTLFL"
     gene            597..1499
                     /locus_tag="VGC82_21135"
     CDS             597..1499
                     /locus_tag="VGC82_21135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0425835.1"
                     /translation="MKGLFLDAVDDLAGVFRHVSRPGDPPMTVNEQGEIKPEDLPALL
                     AGYDFCFDDHTQMPTEAMRACTGLKHVIFLGTGARSYMNPEELAALGITVHTIKGYGD
                     TAVAEHTIALMWAAARGLARMDNGMRAGNWIRTEGMELTGKTIGLLGFGGIAAEVARI
                     ASGSGMRVLAWNRSPKSAPGVTFVDLDTLLAESHVLSIHLLLNDETRGFLSAQRLGRM
                     RPGAILVNTARAAIVDEDAMLAALQAGALRHAALDVFAVEPLPAGHRLTTLPNVTLSA
                     HSAFRTPEASDKLIRMALDIACEL"
     gene            complement(1518..>1765)
                     /locus_tag="VGC82_21140"
     CDS             complement(1518..>1765)
                     /locus_tag="VGC82_21140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY0425836.1"
                     /translation="DVKQVISKNLNGAAEDQRGDYANSDGTMHALLEKQGMVFNEPDP
                     EAFRQVLIKAGFYNKWKDKYGAEAWAVLEKYTGKIGT"
BASE COUNT          317 a          578 c          569 g          301 t
ORIGIN      
        1 aggaacagcg tcttggcgac ctgctgcgtc agcgtggagc cgccctgaac cacgcggccg
       61 gcattgagat tgacccacgc cgcgcgcgcc aggccaatcg gatccaggcc aaaatggtgg
      121 tagaaccgcc gatcctccac cgccaccacc gcagctggca aagagggcga aaaatccttc
      181 aaacgcagcg gctcgccaac cagatcgccg aacgtggcga tgatgcggcc ggcccggtcc
      241 tgcaacgtca gactcggacg ccggaccgca tcgagtgcgg actcggggcg ggggatgtcg
      301 cggcagaacc acaaaaccat gaccgccgcc gccagggtca gccagatcgc ggcgatgaac
      361 ccccaccgca gcaaccagcg caacgcccgc cgcttccggt tcggcgccag cgatgatgga
      421 gccatcgatg tgccccggcc caccggctcg ggcgggtaga accggggaac ggtgggggca
      481 gggcgcggca tcctgattgc gtttagcaga ctctccgtcg cgggcagcga aggcttcttg
      541 ccgcgcttgg catcgttgcg cagagttgtg gcgtttttgc gtcaggagga tgtaccatga
      601 agggcctgtt tctcgatgcc gtggacgacc tcgccggcgt cttccgccac gtctcccgcc
      661 caggcgatcc accgatgacg gtgaacgagc aaggcgaaat caagcctgaa gacctgcccg
      721 cgctgcttgc cggctacgat ttttgtttcg acgaccacac gcagatgccg accgaggcga
      781 tgcgcgcctg caccggcctg aagcacgtca tcttccttgg caccggcgcc cgcagctaca
      841 tgaacccgga ggaactcgcc gcactcggca tcaccgtgca caccatcaaa ggctacggcg
      901 acaccgcggt ggcggagcac acgatcgccc tgatgtgggc tgccgcgcgc gggctggcgc
      961 ggatggacaa tggcatgcgc gcgggcaact ggatccgcac cgaaggcatg gaactgaccg
     1021 gcaagacgat cggcctactg ggtttcggcg gcatcgcggc cgaggtggcc aggattgcct
     1081 cgggctccgg catgcgcgtg ctggcatgga accggtcgcc taaatcggcc ccaggcgtca
     1141 cattcgtcga tctggatacg ctgctggccg agagccacgt gctgtcgata cacctgttgc
     1201 tgaacgacga gacacgcggg ttcctcagtg cccaacggct tggtcggatg cgtcccgggg
     1261 cgatcctggt gaacaccgcc cgagcggcga tcgtggacga ggacgcaatg ctcgcggcac
     1321 ttcaggcggg ggccttgcgc catgcggcgc tcgatgtgtt cgcggtggag ccgctgccgg
     1381 ccgggcacag gctaacaacg ctgccgaacg tgacgctgtc ggcgcacagc gcgttccgca
     1441 cgccagaggc cagcgacaag ctgatcagga tggcgctgga tattgcctgt gagttgtagg
     1501 ttcggtggat ggtaggttca ggtgccgatc ttgccggtat atttctccag caccgcccaa
     1561 gcctcggcgc cgtatttgtc cttccacttg ttgtagaagc ccgccttgat caggacctgc
     1621 cggaacgctt ccggatcggg ttcgttgaac accatacctt gcttttccag cagcgcgtgc
     1681 atcgtcccgt ccgagttggc ataatcaccg cgctgatcct ccgccgctcc gttgaggttc
     1741 ttcgagatca cctgcttcac atccg
//
LOCUS       DASWQN010001243         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_122487, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001243 DASWQN010000000
VERSION     DASWQN010001243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122487"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..759
                     /locus_tag="VGC82_21145"
     CDS             181..759
                     /locus_tag="VGC82_21145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425837.1"
                     /translation="MDETGAKGVLKVFGTTQYGNSGAIINHGVMTASGGLIDLENPTS
                     GNGRIQVNDNGTVKIGNSFAAGGTITINAGTLAFGPAVVSPNPAWATNPGIQFLSAVT
                     FAASDNPALANTLEQITLDAAWHATSSLSFGLSNIQPSMAEMTVFDGNTKIFGAKLLG
                     HYDATEFTLGHAGADVTVIFHTHPLTDAIPHS"
     gene            complement(1100..1654)
                     /locus_tag="VGC82_21150"
     CDS             complement(1100..1654)
                     /locus_tag="VGC82_21150"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY0425838.1"
                     /translation="MQPVSPAATRYDTTTIIFHWATAILVAEQWLGAQIIDWFPRGAL
                     RVDARSVHITCGVLLAIILLGRIVWRLTQGRRLPLAAGPALSVIAKATHWGLYGLLVA
                     MVLVGMALAWTRGDSIFNSFTIPAFDPGNHALADQVQEVHATIGWMIVALAGLHAAAA
                     LFHRYVWRDGVLGRMLPNTRNRLC"
BASE COUNT          435 a          648 c          636 g          365 t
ORIGIN      
        1 atcacagttg acgctcgtga cgggcgcaag cagcgccgcc gcatcgacgc ggtttgccgg
       61 caacattttc gcggtcaaca gcaccctgac caatgaaggt catatccagg tggtcgctgg
      121 caactcctcg gcggaacttg atatacggct gcgctatgca accttgtgaa cttcggcact
      181 atggacgaga ccggcgccaa gggcgtgttg aaggtgttcg gcaccacgca atatggcaac
      241 tccggcgcca tcatcaatca tggggtgatg accgccagtg gtggtctgat cgatctcgag
      301 aaccccacca gcggcaatgg ccggatccag gtaaacgaca acggcaccgt caaaatcggt
      361 aactcattcg ccgccggggg gacgatcacg atcaacgccg gcacgttggc gttcgggcca
      421 gccgtcgtca gccccaaccc cgcctgggcc accaaccccg gcattcagtt cctcagcgcc
      481 gttaccttcg cggcgtccga caaccccgca cttgcgaaca cgctcgagca gatcacgctc
      541 gacgcggcgt ggcatgccac atcgtcgctc agctttggcc tgagcaacat ccagcccagc
      601 atggcagaga tgaccgtgtt tgatggcaac acgaaaatct tcggtgcgaa actgctcgga
      661 cattacgacg cgactgagtt cactctcggg cacgccggcg ccgatgtcac cgtgatattc
      721 catacccatc ctctcaccga cgccattccg cacagttgat ggcaccgtgg cagatcacga
      781 ccgcgggtcg ctcgcgctgg cgcggctggc acagcgttat ggcgtaatgc aacgcatcat
      841 gctgaacgac tgatcagagc cgccgatctg gtcccgacgc ggatgacgta atcggtgttt
      901 cgcaacggcg accgcatttc cggaaattga ctgctacgac cacagcatga ccaagagcag
      961 cgaagacccg agcggccccg cccgaggctg cgtaagacgg cttcaagcac cgtggcactc
     1021 gtcgtcaatc aacgtcatcc acataattct cacggcagcc cgttgcgcgg ctgagcaaaa
     1081 cgcgaaagca caacaccggc tagcaaagtc ggtttctcgt gtttggcagc atgcgaccga
     1141 gcacgccgtc gcgccataca taccgatgaa acaaggccgc cgccgcgtgt aatcccgcga
     1201 gtgcgacgat catccagccg atggttgcat gaacttcctg cacctggtcg gcgagcgcgt
     1261 gattgccagg atcaaacgcc ggaatggtga acgagttgaa gatgctgtct ccgcgggtcc
     1321 acgccaaagc catgccgacc agcaccatcg ccaccagcaa cccatacagg ccccagtggg
     1381 tggctttggc aatcacgctc agcgcgggcc cggcggcgag cggcaatcga cggccttgcg
     1441 tcaggcgcca gacgatccgg cccagcagga tgatcgccag cagcacgccg caggtgatgt
     1501 gcaccgatcg ggcatcaacc cgcagcgcgc cgcggggaaa ccagtcgatg atctgcgcac
     1561 cgagccattg ctcggccacg aggatagccg tggcccagtg gaagatgatg gtcgttgtgt
     1621 cgtagcgtgt tgcggcaggg ctgacaggct gcatggtttg gctcgggctc cccagatgtg
     1681 accattccag aacgcagatc aatgagtttt gccgggtata tgcgagcgtt tcacagcagg
     1741 cgacgccccg gccgccggtt aaagtcccat taccgggccg aaggtggtga agcaggttgt
     1801 ttatggcata acgatgaagt agctggcgcc gccagcagca tatggccgag gttgcatgcg
     1861 ctcaaagggc tgcggtccga ggcacgctgg catttgttga ggctggcgtt cgttgggatg
     1921 gcgtttgttc gggatggcaa cgccgctacg cggcagcagc atccccggcg acagttaagt
     1981 gggcgcgggg acccaggttt cgacgacttc cggcgggtca gatggctgcc gctccgaatg
     2041 gatcatgcca ggcggcagct gggcatgcag agctgccaga gtat
//
LOCUS       DASWQN010001244         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2234162, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001244 DASWQN010000000
VERSION     DASWQN010001244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2234162"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VGC82_21155"
     CDS             complement(<1..519)
                     /locus_tag="VGC82_21155"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY0425839.1"
                     /translation="MARGGRFGSFLLLVVGIVVIGGAALGAWRMWHDKDAALSASREA
                     LAVGVAHGPVVQVVQVTQGPRERLIQLLGDTRSYQTATLYSKVSGYVTSMAVDRGDHV
                     TAGQLLAVVSSVETDQQYESALRDLENKKRNWDRAKELVTRGWTSRQAADDAQTAFTM
                     ATANVGQIATMKS"
     gene            complement(512..>2236)
                     /locus_tag="VGC82_21160"
     CDS             complement(512..>2236)
                     /locus_tag="VGC82_21160"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0425840.1"
                     /translation="LFDRGFERFRGGYGWMLEGTLRNRGIVLAGFTALLVVTGVVAGT
                     IGLDFFPSADVGLIKMHYRAPPGTRIEETERQVLQVEEAVRQIIPAAELDTVNDTVGV
                     PSSFNLAFVPSDNVGAMDAEILISLKSGHHPTIDYIRTIREKVPPQFPGSIVYFQTAD
                     IVSQVLNFGLPAPIDVQIQDVNFERSYGLAQQLVQRMKTIPGVADPHIVQVLNYPGLQ
                     IDVDRMRAAKLRVSQRDVANNMLTSLSSSVLVSPNFFLNPQNNVNYSVAVQTPLSQIK
                     TLDDLLATPVSAPAPLTTADPAALPFASVMRLGDIATVYPRSSLESVNHYTVQRELDI
                     AANVDGRDLGATAADIQAAIKDISKGLPITTHIDIRGQNEVMQASFRSLALGMILAVI
                     LVYALLVILFQSWVDPFIIMMAVPGGLMGIIWILALSGTTINVESLMGSIMTIGISVS
                     NSILVVSFANDLRARGTLDPHASGAMTVFEAAVRACTIRLRPVLMTALAMIIGMLPMA
                     LGLGDAGEQNAPLGRAVIGGLIVATFATLFIVPIFYTLLRRSPPTLHTLDARFAAEAS
                     DTGVTSHG"
BASE COUNT          440 a          735 c          667 g          394 t
ORIGIN      
        1 cgatttcatt gtcgcaattt gcccgacgtt cgcggtcgcc atggtgaaag cggtttgcgc
       61 gtcatctgcg gcttgccgtg acgtccagcc gcgcgtcacc agctccttcg cccgatccca
      121 gttccgcttc ttgttttcca ggtcccgcaa cgcggattca tactgctgat cggtttccac
      181 cgacgagacg acggccagca attgtcccgc cgtcacatgg tcgccccgat ccacggccat
      241 cgaggtcaca tagccgctga ctttgctgta cagcgtcgcg gtctggtaag acctggtatc
      301 gcccagaagc tggatcagcc gctccctggg tccctgggtc acctggacga cctgcacgac
      361 cgggccgtgg gcaacgccca cagccagcgc ctcacgtgag gcggacaacg ctgcgtcctt
      421 gtcgtgccac atccgccatg cgcccaatgc cgcgccgccg atgaccacga tacccaccac
      481 cagcagcaag aatgagccga aacgaccacc tctagccatg cgacgtcact ccagtatcgg
      541 atgcttccgc ggcgaagcgg gcatcgagcg tgtgcagggt cggcggcgaa cgccgcagca
      601 aggtatagaa gatcggcacg atgaacagcg tcgcgaacgt cgccacgatc agccctccga
      661 tcactgctcg gccgagcggg gcattctgct cacccgcgtc gccgaggccg agcgccatgg
      721 gaagcatgcc gatgatcatg gcaagggcgg tcatcaggac cgggcgcaga cggatggtgc
      781 aggcgcgaac cgccgcctcg aacaccgtca tcgcgccaga agcgtgcgga tcgagcgtgc
      841 ccctcgcacg caggtcgttg gcgaagctca cgaccaggat cgagttggaa accgaaatcc
      901 cgatcgtcat gatcgacccc atcagcgact cgacattgat ggtcgtgccg ctgagcgcca
      961 gtatccagat gatccccatc aggccgccgg gcaccgccat catgatgata aacggatcga
     1021 cccaggactg gaacaagatc accagcaacg cataaaccag gatgaccgcc aggatcatgc
     1081 ccagagcaag gctgcggaaa gaggcctgca tcacctcgtt ctggccgcgg atgtcgatat
     1141 gggtggtgat tggcaagcct ttgctgatgt ccttgatcgc cgcctggata tcggctgcgg
     1201 tggcgccgag gtcgcggccg tcgacattgg ccgcgatatc cagctcccgc tgcaccgtgt
     1261 agtggttcac cgactcgagg ctggagcgcg ggtataccgt cgcgatgtcg cccagtcgca
     1321 tgaccgaggc gaagggcaac gccgccggat ccgctgtggt caacggcgca ggcgcactga
     1381 ccggggtggc caacaagtca tccaacgtct tgatctggga cagcggcgtt tgcactgcga
     1441 ccgagtagtt cacgttgttc tgcgggttca ggaagaaatt cggcgacacc agcaccgatg
     1501 aagacaagga tgtcagcatg ttgttggcca cgtcccgctg cgaaacccgc agtttggccg
     1561 cccgcatccg gtccacgtcg atctgcagcc cgggataatt cagtacctgc acgatgtgcg
     1621 gatccgccac gccggggatc gtcttcatcc gctgcaccag ctgttgcgcc aagccgtacg
     1681 agcgctcgaa attcacatcc tgaatctgca cgtctatcgg cgccggcaat ccgaagttca
     1741 gcacctggct gacgatatca gcggtctgga agtaaacgat gctgcccgga aactgcggcg
     1801 gaaccttttc cctgatggtg cggatatagt cgatggtcgg gtggtgcccg ctcttgagcg
     1861 agatcaagat ctcggcgtcc atcgcgccga cgttgtcgct gggcacgaat gccagattga
     1921 acgacgacgg cacgcccacc gtgtcgttga cggtgtcgag ttcggctgcc gggatgatct
     1981 ggcgaaccgc ctcctccacc tgcaacacct gccgctcggt ttcctcgata cgcgtccccg
     2041 gtggcgctcg gtagtgcatc ttgatcagcc cgacatcggc gctcgggaag aagtccagac
     2101 cgatggtccc ggcaaccacg cccgtcacca ccagcaacgc ggtgaagccg gccagcacga
     2161 tgccacgatt gcgcagcgta ccctccagca tccacccata gccgccccga aaccgttcga
     2221 aaccgcggtc gaacag
//
LOCUS       DASWQN010001245         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2630750, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001245 DASWQN010000000
VERSION     DASWQN010001245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2630750"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VGC82_21165"
     CDS             <1..487
                     /locus_tag="VGC82_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012754167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425841.1"
                     /translation="SAWSEARSGADPVVATAAPSGMAKVLKAARDERMDLVIVDTAPH
                     AAPEAAGIARMVDLVLIPCRPTAFDLAAAGNAAAIVKAVGVRTAFVLSACPFRAPEIA
                     EARVALTAYGLPIAPAEITDRRAFARAVATGQSVTEFETDGRAAAEIRALWAWVQEQI
                     Q"
     gene            1167..1697
                     /locus_tag="VGC82_21170"
     CDS             1167..1697
                     /locus_tag="VGC82_21170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425842.1"
                     /translation="MFGDGPRHPLCRERRTVWKARIEIQRRAGQVTDGESYVGQALLK
                     RLGQDGRCDPSHQTLADDSGESVSTVKRALKAFWDCGLVNWVRRLWRNGTRVSQDTNA
                     YVLTLGEPPKFPRVRCEGQFGRETRSVDKSTDHLLVPSTPANERAAANAALVERQRVI
                     EARLLNKRRGSAVLAT"
BASE COUNT          377 a          546 c          546 g          326 t
ORIGIN      
        1 cagcgcgtgg agcgaggcgc gctcgggtgc tgatccggtg gtggcgaccg cggcaccatc
       61 tggaatggcc aaggtgttga aggctgctcg cgatgaacgg atggacctgg tcattgtcga
      121 tactgctccg cacgctgcac ctgaagccgc cggtatcgcc cggatggtcg atcttgttct
      181 tattccctgc cggcccactg cgttcgatct agcggctgcc ggcaacgctg cggcgatcgt
      241 aaaagcggtc ggcgtccgta ctgctttcgt cctctccgcc tgcccgttca gggcgcctga
      301 gattgccgaa gcacgggtgg cgttgaccgc ctacggtctg ccaatagccc cggctgagat
      361 tactgacaga cgtgcgttcg cgagagcggt ggcgaccggc caatccgtta cagagttcga
      421 aaccgacggc cgagctgcgg ccgagatacg tgccctctgg gcatgggtac aggagcaaat
      481 acaatgacca aaaagccaac tggattggcg gcgttcactg gcggccgggg ctccaccaca
      541 gcaggtgaac cggcaacggt gtctaaggcc agcagcagcg gccgaaagag ggggcagggc
      601 gaaaccgtgg cacttaccgt acgagttcgc cggtctgatt gggcgcggct ccggcaactc
      661 gctgatgccg aaggtgaaag cctgcaagtc ctggccgtaa acggcctatc gaaagtgctg
      721 gcggaccatg ggcttcctcc actcgaaccg ctgttatagc gtcaatactt catgacgcca
      781 ttaattcatg acgtaatagt gggacgtagt gtctaaagtt gttaggattt ccaaagaaaa
      841 cagagcgttg cagatcgcga tttgatgagg caagctaccc gataccgcgc ttgtcgcggc
      901 gagtcgtcta ggagctaacc gaaacccaga acccagacat gacaaagccc gccttttgag
      961 gggcgggcct gacatccggc cggagccggg gatattgatc ctgacaaatc aatcatccct
     1021 taatccgggc tctttcgcaa gaggcgtttt tcttgccacg ggagagctgt gcctggtgcc
     1081 tcgcgcacct cggggcggac ctgtcatcag cacaggacac ccgagctatg cccacaccac
     1141 acccacgccc ctggcaccgt gacagcgtgt tcggcgacgg cccgcgccat cccctatgcc
     1201 gcgaacgccg aactgtctgg aaggcccgga tcgagatcca gcgccgggct ggacaggtca
     1261 ccgatggcga atcctacgtc ggtcaggcat tgctcaagcg gctgggccag gacggtcgct
     1321 gcgacccctc ccaccagaca ctggccgatg acagcggaga aagcgtcagc accgtcaagc
     1381 gggcgctgaa ggcgttctgg gactgcggtc tggtgaactg ggtacgacgg ctctggcgca
     1441 acggtacacg cgtgtcacag gacaccaacg cctacgtgct gacgctaggc gaaccgccta
     1501 aattcccacg ggttcgttgc gaaggccaat ttggccgaga aaccagatct gttgataaat
     1561 caacagatca tcttcttgta ccatccacgc cagcaaacga acgtgccgcc gcaaacgcgg
     1621 ccttggtcga gcgacagcgg gtgatcgaag caagactgct caacaagcgg aggggttcag
     1681 cagttcttgc aacctgatcc agcacactcg gtcaatggtc gtctggccat cgcgtccccc
     1741 cgggcagcgt tgacagcgcc atacacaagc acaaggcttg agcctcacct tcgtt
//
LOCUS       DASWQN010001246         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1572904, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001246 DASWQN010000000
VERSION     DASWQN010001246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572904"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VGC82_21175"
     CDS             <1..371
                     /locus_tag="VGC82_21175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HEY0425843.1"
                     /translation="FFFRTILPCSYPIPTDGPVGEMILQTRRHPMRPAHVHFLVNAPG
                     HEPLVTHVFIDGDKYLDTDVVFGVKNDLIAKIEKRSDPVTPDGKPVTAPWHLMTYDFR
                     VKPGQGTAPNPMMAAATEDA"
     gene            701..>1771
                     /locus_tag="VGC82_21180"
     CDS             701..>1771
                     /locus_tag="VGC82_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0425844.1"
                     /translation="MTKPTDNTSRRSLSRRQILKATAGTAALLAAAKLNFPAGAFAEG
                     AGPEIKTAKLGFIALTDASPLFVAKEKGIFAKYGMPDVEVQKQASWGTTRDNLVLGSE
                     GNGIDGAHILTPMPYLISSGKVTQNNQPTPMYILARLNLNGQCISVAKEYADLKIGVD
                     TAPFKSALEQKKASGKSVKAAMTFPGGTHDLWIRYWLAAGGIDPDKDIETIVVPPAQM
                     VANMKVGTMDAFCVCEPWNLQLIHQQIGYTALTTGELWDKHPEKSFGMRAAYVDKYPK
                     AAKALLMAVMEAQQWCEKAENREETAAICAKRQWINVPVEDVTDRMKGKFDYGTGRVV
                     ENSPQQMRFWKDNASYPFQSHDL"
BASE COUNT          379 a          568 c          534 g          290 t
ORIGIN      
        1 ggtttttctt tcgcaccatc ctgccatgca gctacccgat cccgaccgac ggtccggtcg
       61 gcgagatgat cctgcagacc cggcgccatc cgatgcgtcc cgcgcatgtg cattttctgg
      121 tcaatgcgcc gggccacgag ccgctggtca cgcacgtctt tatcgacggc gacaaatatc
      181 tcgacaccga cgtggtgttc ggcgtcaaga acgacctgat cgcgaagatc gagaagcgca
      241 gcgatcccgt gaccccggac ggcaagcctg taacggcgcc ctggcatctg atgacctacg
      301 atttccgggt caagccgggg caggggaccg cgccgaatcc gatgatggct gcggcgaccg
      361 aggacgcctg aaccatcgtg acttgcaccc ttgctcattt cttgtgcgtc gcacaagaaa
      421 tgagcgaatc gcaaatttta ggggcgcggg aaatatctca tccgttcccg gcgggacgcc
      481 taagcaccta gagacgatac gttttttgca ttgcggtagc ttggcacgaa gcttgaatag
      541 tccgtgctga cgccattgta gccgtccatc gaaaccaatc atccgaactg tgacaccgcc
      601 aagcccgggg cctccccgga acgcgccttc atgcgcttcg agcgcgaacc cggtggcacc
      661 agaacggacg gctctgcaca cgcaaaggac gatcgccgcc atgacgaagc ctaccgacaa
      721 cacctcccgc cggagcctca gccgccgcca gatcctgaag gcgaccgcgg gaaccgcggc
      781 gctgctcgcc gccgcaaagc tgaattttcc cgctggcgcg ttcgcggaag gcgcggggcc
      841 cgaaatcaag acggccaaac tcggcttcat cgcgctgacc gatgcgtcgc cgctgttcgt
      901 cgccaaagag aagggcatct tcgccaaata cggcatgccc gacgtggagg tgcagaagca
      961 ggcgtcatgg ggcaccacgc gcgacaacct ggtgctcggc tccgagggca acggcatcga
     1021 tggcgcgcat atcctgacgc cgatgccgta tctgatctcg tccggcaagg tgacgcagaa
     1081 caaccagccg accccgatgt acatcctggc gcggctcaat ctgaacggcc agtgcatttc
     1141 ggtcgccaag gaatatgccg acctcaagat cggcgtcgat acggcgccct tcaaatccgc
     1201 gctcgagcag aagaaggcct ccggcaaatc ggtcaaggcc gcgatgacgt tcccgggcgg
     1261 cacccacgat ctgtggatcc gctactggct cgccgccggc ggcatcgatc cggacaagga
     1321 catcgaaacc atcgtggtgc cgccggcgca gatggtcgcc aacatgaagg tcggcaccat
     1381 ggatgcgttc tgcgtctgcg agccgtggaa cctgcagctg atccatcagc agatcggcta
     1441 taccgcgctg acgacgggcg aactctggga caagcatccg gaaaaatcgt tcggcatgcg
     1501 cgcggcctat gtcgacaaat atccgaaggc ggccaaggcg ctgctgatgg cggtcatgga
     1561 agcccagcag tggtgcgaaa aagcggaaaa tcgcgaagag accgcggcga tctgtgccaa
     1621 gcggcaatgg atcaacgtgc cggtcgagga cgtcaccgac cgcatgaagg gcaagttcga
     1681 ctacggcacc ggccgcgtcg tcgagaacag cccgcagcag atgcggttct ggaaggacaa
     1741 cgcgtcctat ccgttccaga gccacgacct c
//
LOCUS       DASWQN010001247         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1311093, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001247 DASWQN010000000
VERSION     DASWQN010001247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311093"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..580
                     /gene="folK"
                     /locus_tag="VGC82_21185"
     CDS             29..580
                     /gene="folK"
                     /locus_tag="VGC82_21185"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY0425845.1"
                     /translation="MDKFCGHRNGKRSAPALIVLVGVGANLPGLNGRPAIDTCRQAVA
                     MLDSLPDVRLCGLSRWFVSAPVPPSGQPPYVNAVAALLVDPRSTLDPQMLLTRLMAVE
                     TACGRERSVPNAARTLDLDIISIGDLARVAPDPILPHPRAHLRAFVLAPLLDVAPHWI
                     HPVLGKTAAALLADLPPQEIQPL"
     gene            655..1911
                     /locus_tag="VGC82_21190"
     CDS             655..1911
                     /locus_tag="VGC82_21190"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0425846.1"
                     /translation="MAGPVEKEPRLGWLDGLRGLAAMQVVLLHYAFVFLPAIVAIHPL
                     NMYGFSLKGLVGTPLVFMYDGNAAVFLFFIISGVALTHAFAARPLDFPTAVLRRVIRL
                     GLPMAAAVVFAAALFALLPDAHVAASERSGSTALRDIGPREISLPAIIHQIAFEGLLS
                     GFSDFSLLPEWLTKDMGLVPRGQGFNTPLWTLHFEFYGSLLVMLLVGVRASTSRTIYR
                     AGCVLLACAFALSPLGLFIVGHVSAGYLRRVDGRRWQLLVGPAFLGSGILLCTAQIIA
                     PESPLGGLLPPLSSASQAFEQVLQKMLGAILVFGGLALMPVLHRQLERPALRWLGKIS
                     FSLYLSHYPLLYTCAAACFILLSDLSFGAGVAAVTVVGIVLSLLIAVPFERWIDRPAI
                     RLSRMVGGARRRAAAPMMPVAVTEPA"
     gene            complement(2012..2629)
                     /locus_tag="VGC82_21195"
     CDS             complement(2012..2629)
                     /locus_tag="VGC82_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEY0425847.1"
                     /translation="MAQRAAADIPEGWYVNLGIGIPTIIADHVPMEREVIFHSENGVL
                     GIGPAPEKDKIEPWLINAGKQYVTMRAGGSFCHHADSFSMIRGGHLDLCVLGAFQVAD
                     NGDIANWATSENDTAPAVGGAMDLAAGAKRLWVLMEHTTKDGTPKLVTKCTYPLTAPG
                     AVKRVYTNLATLDVTERGFVVLSIAPGLSFENLQARSDAKLHLPA"
BASE COUNT          419 a          814 c          809 g          595 t
ORIGIN      
        1 caggtctgga acagagctca caataaccat ggacaaattt tgcggacatc gaaacggtaa
       61 gcgctccgca cccgctttaa tagtcctagt tggtgtcggc gccaacctac ccggattgaa
      121 tggccggccc gccatcgaca cgtgccgcca agccgttgcg atgctggact cgctacctga
      181 tgttcgcctg tgtggcctct cccgttggtt cgtgtcggca cccgttcctc cttccgggca
      241 gccgccgtat gtcaacgcag tcgccgcact actggttgac ccgcgttcga cactggatcc
      301 acagatgttg ctgacgcgcc tgatggcggt ggagaccgct tgcgggcggg agcgcagtgt
      361 accgaatgcc gcgcggaccc ttgatctgga tatcatctcg atcggcgatt tggcgcgggt
      421 ggcacccgat ccaatcctgc cgcatccacg ggcgcatctg cgggctttcg tgttggcacc
      481 actactcgat gtcgcgccgc attggattca tcctgtcctg ggcaagaccg ccgctgcatt
      541 gctggccgat ctaccaccac aggaaatcca gccgctttga aagatcgaag cggcactgat
      601 accagtttgc aaccggtttc tgattatacc gccagcgaag gcttggagag acgcatggcg
      661 ggaccggttg aaaaagagcc ccgactcggc tggctggatg gactgcgtgg ccttgccgcc
      721 atgcaggtcg tcctgttgca ttatgccttc gtgtttctgc cggcgatcgt tgcgatccat
      781 ccgctgaaca tgtacggctt ctcgctaaaa ggtcttgtcg gcacaccgct cgttttcatg
      841 tatgacggca atgcagcggt gtttctgttc ttcatcatta gtggcgtggc cctgacgcac
      901 gccttcgccg cccgcccgct cgatttccca actgctgtgt tgcgccgggt gatccggttg
      961 ggcctgccca tggccgccgc ggtcgtgttc gctgccgcct tgttcgccct gctgcccgat
     1021 gcgcacgtgg ccgccagcga acgatccgga tcgaccgcac tgcgcgacat cggcccacgt
     1081 gagatttcct tgcctgccat catccatcaa atcgcattcg aaggacttct ttctgggttt
     1141 agcgacttta gccttctgcc tgaatggctg actaaagata tgggtttggt gccaagaggg
     1201 caggggttca atacgccact ttggacgctg catttcgagt tctatgggtc tctgctggtt
     1261 atgctgctgg tcggagtccg cgcctcgacc tcccggacga tctacagagc agggtgcgtg
     1321 ctgctcgcct gcgccttcgc gctgagccct ttgggtctat tcatcgtcgg ccatgtgtct
     1381 gctggctacc tgcgacgcgt cgatggacga cggtggcaac tgcttgttgg gccggcattc
     1441 ctgggttcgg ggatcctgct gtgcacggca caaatcattg ccccagaatc gccattgggc
     1501 gggctgttgc caccgctatc gtctgcatcg caggcctttg aacaggtgct gcagaaaatg
     1561 cttggcgcca tcctggtgtt cgggggcctc gcattgatgc cggtcttgca tcggcaattg
     1621 gagagaccgg ccctgcgctg gctgggaaaa atctccttca gcctttattt gtcgcattat
     1681 ccgctgcttt acacttgcgc cgcggcttgc ttcattcttc tgagcgacct gtcctttggg
     1741 gccggtgtcg ccgctgtgac cgtggtcggc atcgtcctga gcctcctgat cgcggtgccg
     1801 ttcgagcgtt ggatcgatcg cccggcgatc aggctgagcc gcatggtcgg aggggcgcgg
     1861 cggcgcgctg ccgcacccat gatgccggtg gcggtgaccg aaccggcgtg agcaaagaag
     1921 ccatcgaacg ataggatcgt gtgtccctgc tatgagtccc gcggtggatc accaacgggc
     1981 cagggcagcc ggtcgttttg tttcctcgcg gtcaggccgg caaatgcagt ttagcatccg
     2041 aacgggcctg cagattttca aaactgaggc cgggcgcgat gctcagcacg acgaagccgc
     2101 gctccgtcac gtcaagcgtc gcgaggttcg tgtaaacgcg cttgacggct ccgggcgcgg
     2161 tcaacggata ggtgcatttc gtcaccagct tgggcgtgcc gtccttggta gtgtgctcca
     2221 tcagcaccca aagccgcttg gcccctgccg ccagatccat cgcgccgccg acagccggcg
     2281 cggtgtcgtt ttcgctggtc gcccagttgg cgatgtcgcc gttgtcggca acctggaatg
     2341 cgcccagcac gcacaggtcc agatggccgc cgcggatcat cgagaagctg tcggcgtggt
     2401 ggcagaagct gccgccggcg cgcatcgtga cgtactgctt gccggcgttg atcagccagg
     2461 gctcgatctt gtccttctcg ggcgccgggc cgatgcccag cacgccattt tcggagtgga
     2521 agatcacctc ccgctccatc ggcacatggt cggcgattat cgtggggatc ccgatgccaa
     2581 gattgacata ccagccttcg gggatgtcgg cggcggcgcg ctgtgccatt tcggtac
//
LOCUS       DASWQN010001248         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_122927, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001248 DASWQN010000000
VERSION     DASWQN010001248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122927"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1649)
                     /gene="infB"
                     /locus_tag="VGC82_21200"
     CDS             complement(<1..>1649)
                     /gene="infB"
                     /locus_tag="VGC82_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY0425848.1"
                     /translation="ITVQELANRMAARTPEVIKALMKMGVMATVTQTLDADTAELVVQ
                     EFGHRVRRVAESDVELGLEGDADVDDDLFSRPPVVTIMGHVDHGKTSLLDALRATDVA
                     AGEAGGITQHIGAYQVKMPAGELITFIDTPGHEAFTAMRMRGASVTDIVVLVVAADDG
                     VMPQTIEAIRHAKAANAPMIVAINKMDKPDSNPNRVRQELLSYEVVVEEMGGETQDVP
                     VSALKRTGLDKLEEAILLQAEILDLKANPHRSAEGTVIESRLDRGRGPVGTVLVQRGT
                     LMQGDIVVAGSEWGRVRAMLDDKGKQMTVAGPSTPVEILGLSGAPSAGEPFVVVDNES
                     RAREITEFRERRQRDKAAGAAAGARGTLDQMLARIQAGEQKEVAVLIKADVQGSAEAI
                     QVVVTKLAHEEVKVRVLLAGVGQITESDIQLAKASSAVIIAFNVRATSQARELATREG
                     VDIRYFSIIYEVADDVEKLVKGKVAPKAREKFLGYAEVRKVFNITKTGRVAGCYITEG
                     LVKRGTGVRVLRDNVVIHNGELTQLKRFKDDVREVARGYECG"
BASE COUNT          253 a          562 c          505 g          329 t
ORIGIN      
        1 ccgcattcgt agccgcgcgc cacttcgcgc acgtcgtcct tgaagcgctt cagctgggtc
       61 agctcgccgt tgtggattac cacgttgtca cgcagcacgc gcacgccggt gccacgcttg
      121 accaggcctt cggtgatgta acagcccgcg accctgccgg tcttggtaat gttgaacact
      181 ttccgcacct cggcgtagcc caggaacttc tcgcgcgcct tcggggcgac cttgcccttg
      241 accagctttt caacgtcgtc cgccacttcg tagatgatcg agaagtagcg gatgtcgacg
      301 ccttcccgcg tcgccagttc gcgcgcctgc gatgtggcgc gcacgttgaa ggcgatgatc
      361 accgcgctgg acgccttggc caactggata tcgctttcgg tgatctgacc gacgccggcc
      421 aacagtacgc ggaccttgac ctcttcatgc gcgagtttgg tgaccacgac ttggatggct
      481 tcggcgctgc cctgcacgtc ggccttgatc aggacggcga cttccttctg ctcgccggcc
      541 tggatgcggg ccagcatctg gtccagcgtg ccacgcgcac cggcagccgc accggccgcc
      601 ttgtcgcgct gccgacgttc gcggaactcg gtgatctccc gggcccggct ttcgttgtcg
      661 acaacgacga atggttcgcc ggcggacggg gcgccgctga gaccaaggat ttccaccggg
      721 gtcgacggcc cggccacggt catctgcttg cccttgtcgt cgagcatcgc acggacacgc
      781 ccccactcgg agccagccac gacgatgtcg ccctgcatca gggtgccgcg ttgcacaaga
      841 accgtgccga ccggtccgcg accgcgatcc aggcggcttt caatgaccgt gccttccgcg
      901 gagcgatgcg gattggcctt caggtcgagg atttccgcct gcagcagaat tgcctcttcc
      961 agcttgtcca gcccagtccg cttcagcgcc gaaaccggca cgtcctgggt ctcgccgccc
     1021 atttcctcaa ccacgacctc atagctgagc agttcctgcc gcacgcggtt cggattggag
     1081 tccggcttgt ccatcttgtt gatggcgacg atcatcgggg cattggccgc cttggcgtga
     1141 cggatggcct ctatggtctg cggcatgacg ccatcgtcgg ccgccaccac cagcaccacg
     1201 atgtcggtga ccgaggcgcc acgcatgcgc atggcggtga acgcttcatg gcccggcgtg
     1261 tcgatgaagg tgatcagttc acctgccggc atcttcacct ggtaggcgcc gatgtgctgg
     1321 gtaatcccgc cggcctcgcc cgccgccaca tcggtggcgc gcagtgcgtc cagcaaggag
     1381 gtcttgccgt ggtcgacgtg gcccatgatg gtcacgaccg gtgggcggga gaacagatcg
     1441 tcatcgacgt cggcatcgcc ttccagcccg agttcgacgt cggactcagc gacacgccgc
     1501 acacgatggc cgaattcctg caccaccagt tcggcggtat cggcgtccag tgtctgggtg
     1561 acggtggcca tcacccccat cttcatcagg gccttgataa cttccggggt gcgggctgcc
     1621 atacggttgg ccagttcctg cactgtgat
//
LOCUS       DASWQN010001249         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2631163, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001249 DASWQN010000000
VERSION     DASWQN010001249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2631163"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..928)
                     /locus_tag="VGC82_21205"
     CDS             complement(164..928)
                     /locus_tag="VGC82_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY0425849.1"
                     /translation="MSDVLVTQQGPVLEVLFNRPEKKNALTVAMYAAVVDALHRADED
                     RAIRVVLLSGSGDTFTSGNDIKDFQSRAASNEATGASPFLTALSSLAKPLVAAVNGAA
                     IGVGTTMLAHADLVIAAASARFLMPFTSLGLVPEAASSLLFPRLVGHQRASALLLLGE
                     PIDAQTARDWGFVNQVVADADLMTTARSYANRLAALPPASVRQTKDLIKNGRPDVPGR
                     IAEELVLFRERLHSPEAAEAFQAFVEKRKPDFSKFS"
     gene            complement(945..1826)
                     /locus_tag="VGC82_21210"
     CDS             complement(945..1826)
                     /locus_tag="VGC82_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY0425850.1"
                     /translation="MDGPASLPQTDEAGPIRLLAPERPFAPVVFTSAHSGRAYSADLM
                     TGVRLCPLSLRRSEDCFVDELFGAAPAHGAPLLAANFPRAFCDANREAWELDPAMFTD
                     RLPDWVNTTSIRVGAGLGTIAKVVASGEPIYAVKLPFAEAERRVFTYWRPFHDTLLRL
                     IADIRARFGYCLVIDCHSMPSNAPGKRPGARPVEFVLGDLHGVACASRVTRMAETLLS
                     SRGFLVRRNDPYAGGFITKHYGKPADDVHVLQIEIARPLYMDEALFERSSNFPFIQQQ
                     MTDLVAAITLRVHDLIG"
     gene            1945..2421
                     /gene="gpt"
                     /locus_tag="VGC82_21215"
     CDS             1945..2421
                     /gene="gpt"
                     /locus_tag="VGC82_21215"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEY0425851.1"
                     /translation="MAAHYYTVTWDQLHRDARALAWRLMGLGPFTGIVAISRGGLIPA
                     AIIARELDLRLVESVCIVTYQDEKRGEPFVSKAPVAAGDGSGFLIIDDLVDTGTTARV
                     VRGLLPEAYFACVYAKPAGRPTVDSFVTEVSQDTWILFPWDTEPQFIEPLARRNMP"
     gene            complement(2475..>2666)
                     /gene="cpsB"
                     /locus_tag="VGC82_21220"
     CDS             complement(2475..>2666)
                     /gene="cpsB"
                     /locus_tag="VGC82_21220"
                     /EC_number="2.7.7.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421372.1"
                     /note="capsular polysaccharide colanic acid biosynthesis
                     protein; catalyzes the formation of GDP-mannose from GTP
                     and alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEY0425852.1"
                     /translation="TARVTRDAETVILRENESIYLPLGCQHRLENPGRIPLTLIEVQS
                     GAYLGEDDIVRLQDTYGRT"
BASE COUNT          501 a          866 c          843 g          456 t
ORIGIN      
        1 ggaaacaggg ttctcgcgcg gcggcaaagg aagtctgcac gcagaacaat cgtgggtaat
       61 cgccaccaga ccggtcatcg cataaataag cgtcatccgc cccctcctcg ctcgctggac
      121 accctccgcg aagcaagaat ggcgccagcg aacgtgtcgt gcgctaggaa aactttgaga
      181 aatccggctt cctcttttcc acgaacgcct ggaatgcctc cgccgcctcc ggcgagtgca
      241 gccgttcgcg gaatagcacg agttcctcgg cgatccgccc gggcacgtcg ggccgcccgt
      301 tcttgatcag gtccttggtc tggcgcacgg aagccggcgg cagggctgcc agcctgttcg
      361 cataactccg ggcggtggtc atcaggtcgg catcggcgac gacctgattg acgaaacccc
      421 aatcgcgggc ggtttgcgcg tcgatcggct ccccgagcag caacagggca ctggcgcgtt
      481 gatggccgac gaggcgggga aacaacaacg agctggccgc ctccggcacc aggcccaggc
      541 tggtgaatgg catcaaaaac cgagcggatg cggctgcgat cacaaggtcc gcatgcgcca
      601 gcatggtggt gccgacgccg atcgcggcac cattgaccgc cgccaccaag ggcttggcca
      661 gggacgacaa cgcggtcagg aacggagacg cgccggttgc ctcgttcgaa gcggcgcgcg
      721 attgaaaatc cttgatgtcg ttgccggacg tgaaggtgtc gccggaaccg ctcaacagca
      781 ccacccggat cgcgcgatcc tcgtcagccc gatgcaacgc atcgaccacc gcggcgtaca
      841 tcgccacggt cagggcgttc ttcttttccg gccggttgaa cagcacctcc agcaccgggc
      901 cttgctgcgt gacgagtacg tcgctcatgg gattttcctg cctgttatcc gatcaggtca
      961 tgcacccgca gcgtgatcgc cgcgaccaga tcggtcatct gctgttggat gaagggaaaa
     1021 ttgctcgacc gctcgaacag cgcctcgtcc atgtagagcg ggcgggcgat ctcgatctgc
     1081 agcacatgca cgtcgtcggc cggtttaccg taatgcttcg ttatgaagcc gcccgcatag
     1141 gggtcgttgc ggcgaaccag aaagcccctg ctgctcagca gggtctctgc catgcgcgtg
     1201 acccgggacg cgcaggccac tccgtgcagg tcgccaagga cgaattccac cggccgcgcc
     1261 ccgggccgct tcccgggcgc gttggacggc atggaatggc agtcgatgac caggcagtag
     1321 ccaaaccgtg ccctgatgtc cgcgatcagc cgaagcaacg tgtcgtggaa aggacgccag
     1381 taggtgaaaa cccgccgctc ggcctctgca aagggcaact ttaccgcata gatcggctca
     1441 cccgaggcga ccaccttggc gatcgtgccc aggccggcac cgacgcgtat gctggtggtg
     1501 ttgacccaat ccggcaggcg gtcggtgaac atggccgggt ccagttccca cgcttcgcgg
     1561 ttcgcgtcgc agaaggcgcg cgggaaattg gcggccagca ggggcgcgcc atgtgccggc
     1621 gcggccccaa acagctcatc gacgaagcaa tcctcgctgc gccgaagact cagcgggcac
     1681 aaacggacac ccgtcatcag gtcggccgaa taggctcggc cggaatgtgc cgaggtgaaa
     1741 accacggggg cgaacggccg ctccggcgcc agcagccgga tggggccggc ttcatcggtt
     1801 tgcggcaggg atgcggggcc gtccatcgcc ccatcaaaga cggtgtggac caaaatgtca
     1861 ctatattgaa tccagccatc ggtgggtaac ggcggcggcc aaaacccact tgccacggcg
     1921 ggcgatttgc gccgacaaac tggcatggcc gcacattatt acaccgtcac ctgggaccaa
     1981 cttcatcgcg atgcccgtgc cttggcctgg cggctgatgg ggctgggtcc gttcactgga
     2041 atcgtcgcga tcagccgcgg cggcctgatc ccggctgcca tcatcgcccg cgaactcgac
     2101 ctgcggctcg tggaaagtgt ttgtatcgtg acctaccagg acgaaaaacg cggcgagcca
     2161 ttcgtcagca aagcgccggt agcggctggc gacggcagcg ggttcctgat catcgacgac
     2221 cttgtggata ccggcacgac cgctcgggtg gtacgcggtt tgctgccgga ggcgtacttt
     2281 gcttgcgtct atgccaaacc cgcggggcgg ccgacggttg acagctttgt caccgaagtc
     2341 tcccaggata cctggatcct gtttccctgg gacaccgaac cccagttcat cgaaccgctt
     2401 gcccgtcgca acatgccctg acctggcccg gtctgacctg acctgacctg acccggatcg
     2461 gtcaagggcg ggggctacgt ccgtccatac gtgtcctgca gccggacgat gtcgtcttcg
     2521 ccaagatagg cgcccgactg cacctcgatc agtgtcagcg gaatccggcc ggggttttcc
     2581 agccggtgct gacacccaag cggcaggtaa atgctctcgt tctcacgcag gatgacggtc
     2641 tccgcatcgc gcgtgacccg cgctgt
//
LOCUS       DASWQN010001250         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_123133, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001250 DASWQN010000000
VERSION     DASWQN010001250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123133"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VGC82_21225"
     CDS             <1..713
                     /locus_tag="VGC82_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425853.1"
                     /translation="SMSDGLRFPWDVPTLPVAPPANPEPDEPVVRPKRRGRRPASAMR
                     TARAQDAPDWLFHHLTISGPRPAMETFAAAARGSGITPWQLDYAAIEDDIFVRAVSQP
                     ASRRSLTVEGCRILARQFREKVELRQARAAALVGQSLACPFDLHALLPVPDRILHLGP
                     THPETLAWLTTHWGVTDRLRQVSVRDKATTGRRLPAGHSAVGYSFFTDRETPDAAIRA
                     IGAAWPALRFRLERRPLD"
     gene            725..1561
                     /locus_tag="VGC82_21230"
     CDS             725..1561
                     /locus_tag="VGC82_21230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY0425854.1"
                     /translation="MDRLPDPKSGLDEAVADESRAGEGGSPYLTLDGFTGPLDHLLVL
                     ARAQTIDLAAISLTALLDQLSAALHQAPAATSLGQKADWVVMAAWLVQLRTRLLLPAD
                     APGQQEATAEADELRLRLVALEAMQALAFWLEQRPQLGHDVFARGRPELFGVSVEAGP
                     AIDVIEFLWASLALFDDETPPDTAMTYQPAPLQLHTVAQARERILQRLQDQPEGVALD
                     QLLPDEAPDADEPSRRTRLRRSGWAATLVAGLELAKQGEVALGQGEDFEPIHVAPARS
                     TT"
     gene            complement(1654..2493)
                     /locus_tag="VGC82_21235"
     CDS             complement(1654..2493)
                     /locus_tag="VGC82_21235"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0425855.1"
                     /translation="MNPRVLLDGLTFPEGPRWHDGRLWFSDFYSHRVLALSLDGRMET
                     MATVDQLPSGLGWMPDGDLLVVSMLDRKLLRQHSGRLTVHADLATLAGGPCNDMVVDR
                     QGRAYVGNFGYERFQGAAEQPAALIRVDPDGATRVAATGLRFPNGSVITPDGKTLIVG
                     ETMGNRLTQFSIGPDGELAASRTFARVEGLFPDGICLDAEGAVWVADPRGNRVVRVRE
                     GGEIVQTIPLPGRNAYACMLGGADRRTLFICTAPGSGPEREGKTDGKIEIIEVTVPGA
                     GLP"
BASE COUNT          478 a          919 c          868 g          453 t
ORIGIN      
        1 gatctatgag cgacggacta cgcttccctt gggatgttcc gacactgcca gtcgccccac
       61 cggcgaaccc agagccggat gaaccagtcg tcaggccgaa gcggcgtggc cggcgaccag
      121 cttcggccat gcggacagcc cgggcacagg atgcgccgga ctggctgttc catcatctga
      181 ccatcagcgg gccaaggccg gcgatggaga ccttcgcagc tgcggcgcgc gggtccggga
      241 tcacgccctg gcagctggat tacgccgcca tcgaggacga catttttgtc cgtgcggtgt
      301 cccagccagc cagtcgcagg agcctgacgg tggaaggctg ccgcatcctg gcgcggcaat
      361 ttcgcgaaaa ggtggaactc cgtcaggcgc gcgccgcagc gctggtcggc cagagcctcg
      421 cctgcccgtt cgacctgcat gcgctcttgc cggtgccgga caggatcctg catctcgggc
      481 ccacgcatcc agagactctg gcctggctaa caacgcactg gggcgtcacc gaccggctgc
      541 gccaggtcag cgtgcgcgac aaggccacca ccggccgacg gctgccggca ggccactcgg
      601 cggtcggcta cagcttcttc accgatcgtg aaacgcccga tgcggcgatc cgtgcaatcg
      661 gggcagcctg gccggcgcta cggtttcggc tcgagcggcg gccgctggac tgaaccagac
      721 ggccatggac agactccctg atccgaagag cgggcttgat gaggcggtgg cggacgaatc
      781 ccgggcaggg gagggaggtt caccctatct gacgctcgac ggctttaccg gtccactgga
      841 ccatctgctc gtgctggcgc gtgcccagac aatcgacctg gcggcaattt cgctgaccgc
      901 gctgctcgac caactgagcg cggcgctgca ccaggcaccg gcggcgacat cgctggggca
      961 gaaagccgac tgggtggtga tggcggcctg gctggtgcag ctgcgcacgc gcctgctgtt
     1021 gccggcggac gcgccgggcc agcaggaggc cacagcggaa gcggacgaac tccgcctccg
     1081 tctcgtcgcg ctggaggcga tgcaggcgct cgccttctgg ctggagcagc gcccgcaact
     1141 cggtcacgac gtgttcgcgc gcggacggcc tgagctgttc ggcgtttcgg tcgaggccgg
     1201 accggcgatc gacgtgatcg aattcctctg ggccagcctc gcactgttcg acgacgagac
     1261 accgccggac acggcaatga cctatcagcc cgcgccgcta cagcttcaca ccgtcgcgca
     1321 ggcccgcgag cgcattctgc agcggctgca agaccaaccc gagggcgtcg cactcgatca
     1381 gctcctgcct gacgaagctc cagacgccga cgagccatcg cgccggaccc ggctgcggcg
     1441 ctccggctgg gcggcgacac ttgtggccgg gctggaactg gccaagcagg gggaggtggc
     1501 gctggggcag ggggaggatt tcgaacccat ccatgtggca ccggctaggt ccacgaccta
     1561 atcgtcagaa gcgctatagc ctgtgcagtc cagcggcgga tattgttgcc gttcaaagca
     1621 cctatgccga acacggccac tgcattcaga cagtcacggc agtcccgcgc ccggcacagt
     1681 aacctcgatg atctcgatct tgccgtcggt tttgccttca cgctctggtc cgctgccggg
     1741 cgctgtgcag atgaataagg ttcgacgatc cgctccaccc agcatacacg cataggcatt
     1801 ccgaccaggc aacgggatcg tctggacgat ctcgccgcct tcccgcactc ttacgacgcg
     1861 gttaccgcgc gggtcagcca cccagacggc gccctctgca tccaggcaga tcccgtccgg
     1921 aaacaagcct tcgacccggg cgaacgtccg agaggctgca agctcaccgt ccggaccaat
     1981 cgagaactgc gtcaaccggt tgcccattgt ctccccgaca atcaaggttt tgccgtcggg
     2041 cgtgattacg ctgccattcg ggaagcgcag ccccgtggct gccacacgcg ttgcgccgtc
     2101 tggatcgacg cggatgagcg ccgccggctg ttccgccgca ccctggaacc gctcgtagcc
     2161 gaagttgccg acgtaggctc gtccctgacg atccaccacc atgtcattac atgggccgcc
     2221 tgcaagcgtc gccaaatcgg cgtgcaccgt taaccggccg ctgtgctggc gcaacagctt
     2281 gcggtccagc atggacacta ccagcaggtc tccgtccggc atccagccca ggccggaagg
     2341 caattggtcc accgttgcca tcgtttccat gcggccgtcc agcgacagcg ccagcacgcg
     2401 gtgcgagtag aagtccgaaa accacaagcg gccatcatgc cagcgcgggc cttcgggaaa
     2461 agtcaggccg tctagaagta cgcgtggatt catggcgtgg tttcctctgt tcgaggatac
     2521 ttcacgcgtc ggacgtattc tggtcaaacc ctaatcttac tgtgtcattt tctcattcac
     2581 tgattgcgtc tgttgctgca gacagcacgg acatctgggc aacgtcctga ccagccggat
     2641 ggcaagctga cgacggatgc tcgcgatcga gtgcggcacg tgccgctcag ggcgcaccgg
     2701 cagctccccg cggctgga
//
LOCUS       DASWQN010001251         4403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2497604, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001251 DASWQN010000000
VERSION     DASWQN010001251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2497604"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..>4403
                     /locus_tag="VGC82_21240"
     CDS             50..>4403
                     /locus_tag="VGC82_21240"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY0425856.1"
                     /translation="MNAAPIAIIGAACRLPGAPGLDALGELLFAGRDAVGEVPPGRWA
                     RELFYHPAAGQPGKAFTMAAGCIDAIDRFDAAFFGISAREAAFIDPQQRLLLELAHET
                     LEDAGVPAFRLAGQSVGVFVGGSSWDYATIAAADLLGTDNSSMQGVALSSMSNRLSYQ
                     FDWRGPSLTIDTACSSSLVALHMACEAIRRSEVPIALVGGVNLILAPHPFVGFARAAM
                     LSRRGRCQAFDAKADGYVRAEGGGAVLLKPLRDAIADGNPIRAVILASGVNSDGRTNG
                     FSVPSTVAQERLLREVHARAGIAPDDISYLEAHGTGTAVGDPLEASAIGRALGRYRRV
                     PLPIGSVKSNIGHLEAASGMAGLMKLIASLDRREIPASLHFETPNPHIPFAALKIEVV
                     TAPRPMIQGAAGYVAGLNSFGFGGTNAHVVLAAPPDSRAEAAGASPMPADEGLPPLLL
                     SARAPKALQAVAQSWQRTLTTLPPDALVPLLRGAARLRDHHPHRLAIPGAPAVMMASS
                     LRAWLDDPDALSGHNPAAQGKVAFVFAGNGSQWPGMASDAITRSRPFQQMLSEVDAVL
                     TPLLGWSVKARLRQPAGAGDLRDTAIAQPLLFAVQLGCVAALRQHGVVADAHVGHSVG
                     EVAASWAAGALDLEQAARVIVARSMAQQSRHGIGGMAVLGLGAEDAAAAIARSTPDLA
                     IAAINSARSVTIADQEPALAELQRYAEAMGWLCFPLDIDYAFHSAAMDPIRDTVVGSL
                     AGLEPIVPTTMLVSTVTGQEIIEPLMDAEYWWRNVREPVRFSDAVETLIASGVHLFLE
                     IGPQPVLQSYLHEAIKRSAVPGRVMSTLARSREETDPFPAIAARCYVAGCSLHDAPAF
                     EGPSTPRGLPSYPWQRERFWHDRTPGTHEIIAPARDHPLLGYRQATAPGAWFSHPSTA
                     TERWLQDHVVDGAAVMPAAGMIEMALAAARTLYPGAASLELRDLEITRTLPLEQDASH
                     EVTVHVGSDGEGLTIASRSLLSADEPIVHAAGRIFRGTKTPTVFDLPAGRGNLDTGNA
                     VEICAATVYQAAAALQLSYGPAFRTVRRVLASGSGRAVVELDHLGVDRVAEGYLVDPA
                     LVDGALQGLIGLAATRPSGREREAVLPWRFGRVRLLRSDGVRPVKAVLHLRQRGVRTL
                     LADIALLDADDVVVMELLECWFATADFGAAPEPAERMTWTALVPSLRQPDGAIADWLD
                     PVFDAVAGAAATPETVILAEAMLALAARDAVAAGLQPNEHLLAPPADRRLAHMLHWLA
                     QDGLAQRDAVAQRDGLAQRDGTCWRIAEATDLPRWQDVWRDLLFDVPAAAADAALAGA
                     VAQALRPAGGQQATQTLPTSLVDQMLFASPTGGGAIDALLAGVEAWLDHWPAGRPLRV
                     AEIGVAWPGLTRQLLGLLARRVPAMRYVALAADQDVLAALAESLADWPGATGNVWDRA
                     AEQEEFDLVLGLYPF"
BASE COUNT          653 a         1416 c         1509 g          825 t
ORIGIN      
        1 gggcaattta tgtggcctga ccaatgatta tgacccgcct gttgcttctg tgaacgccgc
       61 accgatcgcc atcattggcg ccgcctgccg cttgccaggg gcgccggggc tcgatgcgct
      121 tggcgaactc ctgttcgccg gccgcgacgc ggttggcgaa gtgccgccag gccgttgggc
      181 ccgagaactg ttttaccatc cagcagccgg gcagcctgga aaagcattca ccatggcggc
      241 gggctgcatc gacgcgatcg accgtttcga cgctgcgttt ttcggcatat cggcgcgcga
      301 agctgctttc atcgatccgc agcagcgcct tcttctggaa ctggcgcatg agacgctgga
      361 ggacgcgggc gttcccgctt tccggcttgc cgggcaaagc gtcggcgtct tcgtcggtgg
      421 gtcgtcctgg gattatgcca caatcgcggc ggccgatctg ttgggcaccg acaattcctc
      481 gatgcagggt gtggcgcttt cctcgatgtc gaaccggttg tcctatcagt tcgattggcg
      541 tggaccgagc ctgacgatcg ataccgcctg ctcgtcctcg ttggtcgcgc tgcacatggc
      601 ctgcgaggcg atccgccgtt cggaggttcc gatcgccttg gtcggtggcg tcaacctgat
      661 cctggcgccg catcctttcg tgggctttgc ccgggccgcg atgctgtcgc gccgtggccg
      721 ctgccaagcc ttcgacgcca aggccgacgg ctatgtgcgt gctgagggcg gcggcgccgt
      781 gttgctgaag ccgcttcggg atgccatcgc cgacggcaac ccaattcgcg cggtgatctt
      841 ggcatccggg gtcaactcgg acggacggac gaacgggttt tccgtgccga gtactgtggc
      901 acaggaacgt ttgttgcgcg aggtgcacgc gcgcgccggc atcgcgcctg atgacatcag
      961 ctatcttgaa gcgcacggga ccggcacggc ggtgggcgat cccctggaag ccagtgcgat
     1021 cggcagggcc cttgggcgat accggcgtgt cccgctgcca atcggttcgg tgaaaagcaa
     1081 tattggccat ctcgaagccg ccagcggcat ggccggtctg atgaagctga ttgcctcatt
     1141 ggatcggcgc gaaattccgg cttcgctgca cttcgagact cctaacccgc acatcccgtt
     1201 tgcggccctc aagatcgagg tcgtcaccgc gccgcggccg atgatccaag gagcggcggg
     1261 atacgtcgcc ggcctgaact cgttcggttt cggcggcacc aacgcccatg tcgtgctggc
     1321 tgcaccgccg gattcccgtg ccgaagccgc cggtgcatcc ccaatgccgg cagacgaagg
     1381 attgccgccg ctgctgttgt cggcgcgcgc accgaaggcc ctgcaagccg tcgcgcaatc
     1441 gtggcagcgc acgctgacca ccctgccgcc cgatgccctt gtcccactgt tgcggggtgc
     1501 cgcgcggctg cgcgaccatc atccgcatcg tttagctatt ccaggggcgc cagccgtaat
     1561 gatggccagt tcactgcggg catggctgga cgatcctgat gccctgagcg gtcacaatcc
     1621 cgccgcgcag ggcaaagtag cattcgtttt tgccggcaac ggcagccagt ggcccggcat
     1681 ggcaagcgat gcgatcacgc gcagccggcc ctttcaacaa atgctcagcg aggtggatgc
     1741 ggtgctgacg ccgctgctgg gatggtcggt caaggcccgt ttgcgccagc cagcgggcgc
     1801 cggcgatctg cgcgataccg ccatcgccca gcccctgctg ttcgccgttc agcttggttg
     1861 cgtcgccgcg ctgcggcagc acggggtggt cgccgatgcc catgtcggac acagcgtggg
     1921 cgaggtcgcc gcttcctggg ctgcgggcgc gcttgacctg gaacaggcgg cacgggtcat
     1981 cgtcgcgcgc agcatggcac agcaatcgcg tcacggcatc ggcggcatgg cggtgctggg
     2041 gcttggcgcc gaggacgcag ccgcggcgat tgcgcggtcc acgcccgatc ttgccatcgc
     2101 ggccatcaac tccgcccggt cggtgacgat cgccgaccag gaaccggcac tggcggagtt
     2161 acagcgctac gccgaggcga tgggctggct gtgtttcccg ctggacatcg actacgcctt
     2221 ccacagtgcc gcgatggatc cgatccgcga taccgtggtc ggcagccttg cgggcctgga
     2281 accaattgtc ccgacgacaa tgctggtctc caccgtcaca ggccaggaaa tcatcgagcc
     2341 gctcatggac gcggagtact ggtggcgcaa cgtgcgcgaa cccgtgcggt tcagcgacgc
     2401 cgtggaaaca ttgatcgcct ccggtgtcca tctgttcctg gagatcgggc cgcagccggt
     2461 gctgcaatcc tatctgcacg aagcgatcaa gcgcagcgca gtgcccggac gcgtcatgag
     2521 caccctggcg cgaagccggg aggagaccga tccgtttccc gcgattgcgg cacgctgcta
     2581 tgtcgctggg tgcagcctgc atgacgcgcc cgcgttcgag ggaccgtcaa cgccgcgtgg
     2641 cctgccctcc tatccttggc agcgcgagcg tttctggcac gaccggactc cgggaacgca
     2701 tgaaatcatc gcgccggccc gcgatcatcc gctgcttggt tatcgccaag ccacggcacc
     2761 gggagcctgg ttcagtcacc cttcgaccgc gacggagcgt tggctacagg accatgtcgt
     2821 cgatggagcg gccgtgatgc cggcggccgg aatgatagag atggccctgg ccgccgcccg
     2881 gacgctttat cctggtgccg cgagcctgga gctgcgtgac ctcgagatca cccgtacctt
     2941 gccgcttgaa caggacgcgt cgcatgaggt gacggtgcat gtcggttccg acggcgaggg
     3001 gcttaccatc gccagtcggt cacttctgtc cgccgacgag cctatagtgc atgctgccgg
     3061 ccggattttc cgcggcacca agacaccgac ggtattcgac ctgcccgccg gccgcggaaa
     3121 cctcgatacc gggaacgcgg tcgaaatctg tgcggcgacg gtttatcagg cggccgcggc
     3181 attgcagttg tcctatggtc cggccttccg aaccgttcgc cgcgttctgg cgtccggttc
     3241 gggacgcgcg gtggtggagc ttgaccacct cggcgttgac cgggtggcgg agggttacct
     3301 tgttgacccc gctctggttg acggggcgct gcaggggttg atcggcctcg ccgcgacccg
     3361 gccatcagga cgggaacggg aagctgtgct gccctggcga tttgggcggg tgcggctgct
     3421 gcgctccgac ggtgtccggc cggtcaaggc agtgctgcac ctgcgtcagc gcggtgtgcg
     3481 gacgctcttg gccgatatcg ccctgctcga tgccgacgat gtggtggtga tggagttgct
     3541 ggagtgctgg ttcgcgaccg cggattttgg cgcggcgcca gagccggctg agcggatgac
     3601 ctggacggcg ctggtgccaa gcctcaggca gccggatgga gccattgcgg actggctgga
     3661 tcccgtcttc gatgcggttg ccggtgcggc ggctacgccg gagaccgtga tactggccga
     3721 ggcgatgctg gcactggcgg cgcgtgatgc ggtcgcagcc gggttgcagc caaacgagca
     3781 cctgttggcg ccaccggccg atcggcggtt ggcccacatg ctccactggc tggcacagga
     3841 tggactggcc caacgtgatg cagtggccca gcgtgatgga ctggcccagc gcgacggcac
     3901 ctgttggcga atcgccgaag ccacggatct tccgcggtgg caggatgtct ggcgagacct
     3961 tctgttcgac gtgccagccg ccgcggccga tgcggccctt gccggcgccg tggcgcaagc
     4021 gctgcgtccc gccgggggtc aacaggcgac acaaactctt ccgacgtcgc tggtcgatca
     4081 gatgctgttt gcctcgccca cgggcggcgg cgcgatcgat gcgctgcttg ctggggtcga
     4141 agcctggctc gatcattggc cggcgggccg gccgttgcgg gtagcggaaa taggcgtggc
     4201 ttggcccggt cttacgcgac aacttctcgg tctcttggct cgtcgtgtgc cggcaatgcg
     4261 ctatgtcgcg ctggcggccg accaggacgt gctggccgcg cttgccgaat ccttggcgga
     4321 ctggcccggg gccaccggca atgtctggga ccgcgcggcg gagcaggagg aattcgacct
     4381 tgttttaggt ctttatccat tca
//
LOCUS       DASWQN010001252         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_255536, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001252 DASWQN010000000
VERSION     DASWQN010001252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255536"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /gene="istA"
                     /locus_tag="VGC82_21245"
     CDS             complement(<1..1149)
                     /gene="istA"
                     /locus_tag="VGC82_21245"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY0425857.1"
                     /translation="MDMIAEIRRRHLVSKESISSIARALKLSRPTVRKHCRSQCEPIY
                     QRHKQPTPMLGAFEETLETWLRSERLLPKAQRRSARRLFEGLQAEGYRGAYDSVQRFV
                     QRWKVAKSGPALTQAYVPLAFAPGEVCQFDWSHEHVELAGVMQTIKVAHFRLAFSRQM
                     FVVAYPRETQEMVFDAHNRAFAFFGGVPERMVYDNLKAVVETIFTGKERLFNRRFMVL
                     ANHYLFEPVACTPASGWEKGQVENQVGNIREWLFTPLARFADFAALNAWLATRCWELG
                     QRKHPVTPEQSIATCFIAEQLALRRITAPFDGYVEHMLRVSSTCLVVLDRNRYSVPAA
                     FAGRAVSVRSTASEIRIVADGAVIAEHARRFGRDQLICDPWHYLPILER"
     gene            complement(1259..>1567)
                     /locus_tag="VGC82_21250"
     CDS             complement(1259..>1567)
                     /locus_tag="VGC82_21250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425858.1"
                     /translation="EDIGGGLMATKPLPPIDAELVQPRPRPNLANLRPREEISDSAIE
                     ANSRSIGGQWRASTQLPQLEEATPLASIRIDIPEYVDKQLKLRIVENGGTKALYILKV
                     "
BASE COUNT          299 a          500 c          475 g          293 t
ORIGIN      
        1 gcgttccagg atcggcaggt agtgccaggg atcacagatc aactgatcac gcccgaagcg
       61 gcgcgcgtgt tcggcgatca ccgcgccgtc ggccacgatg cggatctcgc tcgccgtgct
      121 gcgcaccgag accgcacgcc cagcaaacgc cgccggaacg ctatagcggt tgcggtccag
      181 caccacgagg caggtgctgg agacccgcag catatgctcc acatagccat cgaacggcgc
      241 ggtgatccgc cgcaaagcaa gctgctcggc aatgaagcaa gtggcgatcg actgctccgg
      301 ggtcaccgga tgcttgcgct gccccagttc ccagcagcgc gtcgccagcc atgcgttcaa
      361 cgccgcgaag tcggcaaacc gtgccagcgg cgtgaacagc cattcgcgga tgttgccgac
      421 ctggttttcg acctggccct tctcccagcc tgacgccggg gtgcaggcga ccggctcaaa
      481 cagataatgg ttggccagca ccatgaaacg gcgattgaac aggcgttcct tgcccgtgaa
      541 gatcgtttcc acgaccgctt tgaggttgtc ataaaccatg cgctcgggca cgccgccaaa
      601 gaaggcgaag gcgcggttat gcgcgtcgaa caccatctcc tgggtttctc gggggtaggc
      661 cacaacaaac atctggcggc tgaatgccag acggaaatgc gcgaccttga tggtctgcat
      721 cacgcctgcg agctcgacat gttcgtggct ccagtcgaac tggcagacct cccccggcgc
      781 aaacgccagc ggcacatagg cttgcgtcag ggcaggacca gatttggcga ccttccagcg
      841 ctgcacgaac cgctgcacgc tgtcataggc accgcgatac ccctcggcct gcaagccctc
      901 gaacaaccga cgcgcggagc gccgttgggc tttcggcagc aggcgttcgc ttcgcagcca
      961 ggtctccagc gtctcctcga acgcgcccag catcggcgtc ggctgcttgt ggcgttggta
     1021 gattggttcg cactggctgc ggcaatgctt gcgcaccgtc gggcgagaca gtttcagggc
     1081 acgcgcaatc gagctgatgc tctccttgct gaccaaatga cggcgccgga tctcggcaat
     1141 catatccatg aacaacatcc cagattctcc ggccaaaccg ccggatgatc gcatgaacca
     1201 gggtggaaac tattgggcgc tgtttacccc cggaatctgg aaagttttgc acgctgtttt
     1261 acaccttgag gatataaagg gccttcgtgc cgccgttctc gacgatcctg agcttcaact
     1321 gcttgtccac atattccgga atgtcgatcc ggatgctggc cagaggcgtc gcctcctcca
     1381 gctgaggcag ctgcgtcgac gctcgccatt ggccgccaat cgagcgggag ttcgcctcga
     1441 tggcgctgtc ggatatctcc tcgcgcgggc ggaggttggc aaggttcgga cgtggccgtg
     1501 gctgcacaag ttcagcgtcg atgggcggca gaggtttcgt cgccattaag ccgcctccga
     1561 tatcttc
//
LOCUS       DASWQN010001253         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2234893, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001253 DASWQN010000000
VERSION     DASWQN010001253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2234893"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..535)
                     /locus_tag="VGC82_21255"
     CDS             complement(80..535)
                     /locus_tag="VGC82_21255"
                     /inference="COORDINATES: protein motif:HMM:NF022612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HEY0425859.1"
                     /translation="MPAPEQLRLVALDADDLSVISAQVQDARIQAADIVWRQDEKRLV
                     VGLNRLDWEQTLSGGTAPRRLLAALRFDRVLACKSRNIDLEQLDAVLELVGIEFHPAE
                     PPGGSALLLFSHGGALRLDVECLECELTDLGGDDLGTSDVDAESSGMGA"
     gene            complement(728..1387)
                     /locus_tag="VGC82_21260"
     CDS             complement(728..1387)
                     /locus_tag="VGC82_21260"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY0425860.1"
                     /translation="MKVILFGATGMVGQGVLRECLLDAGVESVLVVGRSPSGQQHAKL
                     REIIHGDFTDFSAIESQLAGYDACFFCLGVSSVGMEAERYRHLTYDVTMAAAKTLSRL
                     NPNLVFTYVTGRGTDSTERGPQRWARVKGKTENDLLKLPFKAAYMFRPGGIQPLHGVR
                     SKTAWVQAIYVVAAPLWSYLARTQPQFMTTSEKLGRAMIKVARDGYPKRILESADINS
                     I"
     gene            complement(1384..>1909)
                     /locus_tag="VGC82_21265"
     CDS             complement(1384..>1909)
                     /locus_tag="VGC82_21265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0425861.1"
                     /translation="VVHYIARHGESRVAKAAILSAVPPLMVQTAANPGGLPKSVFDGL
                     QQALAASRTQFYRDLPSGPFYGYNRPGAKPSEAVIQNWWRQGMMGGAKAHYDGIVAFS
                     QTDFTEDLKKITVPVLVMHGDDDQIVPYADSAPLSAKLLKNGTLKTYKGFPHGMPTTE
                     AATINADLLAFIKS"
BASE COUNT          333 a          620 c          620 g          336 t
ORIGIN      
        1 tgggcaggct gtagcaatac cgcgcgtgac cacaactgac cgggattatg ggtccccgcg
       61 ttcgcggggg cgacggaaat cacgccccca tccccgacga ttccgcgtcc acatcgctgg
      121 tgccgaggtc gtccccgccg aggtcggtca gctcgcattc caggcattcg acgtcgagcc
      181 gcagtgcccc gccatggctg aacagcaaga gcgcacttcc gccggggggc tcggctgggt
      241 ggaattcaat cccgaccagt tccagcacgg catcgagctg ttccaggtcg atgttgcggg
      301 atttgcaggc cagcacccgg tcgaaacgaa gcgcggcgag cagccggcgc ggggcggttc
      361 cacccgacag ggtctgttcc cagtcgagcc ggttcagccc gaccaccagc cgtttttcgt
      421 cctgccgcca gacgatgtcg gcggcctgga tccgggcgtc ctggacttgc gccgagatca
      481 ccgaaagatc gtcggcgtcg agcgcgacca gtctgagctg ttccggcgcc ggcatcgtca
      541 aacccctttg ctggcgtcga tcatgcgaat gcggtccatc aaggcgcaag ctacaggagg
      601 tgtgcggcca aggcaaaagg cgcggggttc caccgtcatt ccggggcgcg cgccgcgcga
      661 acccggaaga tcgaggatac gatctcgaga ttccgggttc agtcctgcgg actgccccgg
      721 aatgacatca gatactgttg atatccgcgc tctccaggat ccgcttcgga tagccgtcgc
      781 gcgccacctt gatcatggcg cggccgagct tttcgcttgt tgtcatgaac tgcggctggg
      841 tccgcgccag atacgaccac agcggcgcgg cgacgacgta gatcgcctgc acccaggcgg
      901 tcttggagcg gacgccgtgc agcggctgga tgccgccggg gcggaacatg taggccgcct
      961 tgaacggcag cttgagcagg tcgttttcgg tcttgccctt gacgcgcgcc cagcgctgcg
     1021 ggccccgttc ggtcgagtcg gtgccgcggc cggtgacata ggtgaacacc aggttcgggt
     1081 tgagccgtga caacgtcttc gccgccgcca tggtgacgtc gtaggtcagg tgacggtaac
     1141 gctccgcctc catgcccacc gacgaaacgc cgaggcagaa gaagcaggcg tcgtagccgg
     1201 caagctggga ctcgatcgcg gaaaaatccg tgaaatcgcc atggatgatt tcacgcagct
     1261 tggcatgctg ctgccccgac gggctgcgcc cgacgaccag cacgctctcg accccggcat
     1321 cgagcaggca ttcgcgcaac acgccctgcc cgaccatgcc ggtggcgccg aacaggatca
     1381 ccttcatgat ttgatgaaag caagcaggtc cgcgttgatc gtcgccgctt ccgtcgtcgg
     1441 catgccgtgc ggaaagccct tgtaggtttt cagcgtgccg ttcttcagca gtttggccga
     1501 cagcggcgcc gagtcggcat agggcacgat ctggtcgtca tcgccatgca tcaccagcac
     1561 cggcaccgtg atcttcttga ggtcttcggt gaaatcggtt tgcgagaacg cgacgatgcc
     1621 gtcgtaatgc gctttggcgc cgcccatcat gccctgccgc caccagttct ggatcacggc
     1681 ctccgacggc ttggcgccgg gacggttgta gccatagaac ggaccggagg ggagatcgcg
     1741 gtagaattgc gtgcggctgg ccgccagcgc ctgctgcaag ccgtcgaaca ccgatttggg
     1801 aagaccgccg ggattggccg ccgtctgcac catcagcggc ggcaccgcgc tgagaatggc
     1861 cgccttcgcc acacgagatt cgccgtggcg cgcgatgtaa tgcaccacc
//
LOCUS       DASWQN010001254         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1311568, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001254 DASWQN010000000
VERSION     DASWQN010001254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311568"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VGC82_21270"
     CDS             complement(<1..500)
                     /locus_tag="VGC82_21270"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEY0425862.1"
                     /translation="MTDIAIIGAGPYGLSLAAHLSAAGLNVRVFGQPMNTWRTSMPEG
                     MVLKSEGFASNLWHPDGAFTLRDYCAEQGLPYQDSGLPVPLETFSDYGVAFQKRFVPT
                     LDERWVTGLDREDDAFVLRLQDNEIVRAQRVVVAAGIGSFHYTPEELEPIKGPLCSHS
                     IDHHFL"
     gene            694..>1917
                     /locus_tag="VGC82_21275"
     CDS             694..>1917
                     /locus_tag="VGC82_21275"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY0425863.1"
                     /translation="MPTALATAFATPEVIEDATRDDGGTPEIGPRVKQDPHFLLATTG
                     QLPSTARLAMELHDAGARVSLIAPKNHPGWALDLLSHRKTYRALTAPRCLEAALLRFR
                     PDLVIPCDERAVRDLHRIWRDTSDPFVKQLVEKSTAPAEGFPTITSRAELLSLAQRHG
                     VRIPASMPLADVMALDQWTSDNAAPFVLKADGSWAGFGVRIISNAATAKDAFRRMTKP
                     VSLRLALRESLLEGNNFDIRSCLRRERRAMSVQGFVDGWPANIGVACWQGEVLATICA
                     ESVATTSTTGPSTVARVIDNAEMVDAAGRIVAALGLSGMVGFDFMIEAASGAAYLIEM
                     NPRNTPICAVRLGEGRDLAEALVARIANRPRRQRPPRTSRDIVVFFPDTWREDPANQF
                     LYSEYHDVPWEQPELV"
BASE COUNT          386 a          579 c          580 g          372 t
ORIGIN      
        1 tccaggaaat ggtgatcgat gctgtgggag cagagcgggc ctttgatcgg ctcgagttct
       61 tccggcgtat agtgaaagct gccgatgccg gccgcgacga ccactcgttg cgccctgaca
      121 atctcgttgt cctgcagccg caacacgaat gcgtcatcct cacggtccag tccggtaacc
      181 caacgctcgt ccaacgtagg gacaaagcgt ttctggaacg cgacgccgta atccgagaat
      241 gtttccagcg gaacggggag gccggaatcc tgatagggaa gtccctgttc cgcgcagtag
      301 tcgcgcaatg tgaaagcgcc gtccggatgc cagagattcg acgcaaaacc ttcggatttc
      361 aacaccatgc cttcgggcat gctggtgcgc caggtgttca tcggctgacc gaacacgcgc
      421 acgttgaggc ccgcggcgga caaatgcgcg gcaagcgaca ggccgtaggg gccggcgccg
      481 ataatcgcga tatccgtcat cttgcgcatg aacggcctga ttctaaccgt gacgtgaaca
      541 gatccatcac aagcacctca tcactatggt aatcgctttt atcgacgttg cgcctgtggt
      601 cttctgacgc aagggcaaca taagcattga ttgcagaacg gaccgtatat tgtccagtaa
      661 ctcactcgta aatgatggta aggacagggt ctgatgccca cggcgttagc aacggcattc
      721 gcaacacccg aggtgatcga ggatgctacc cgtgatgacg ggggcacccc ggaaatcggg
      781 cctcgggtga aacaggatcc gcatttcctg ttggcgacga ccggccaact gccgtcaacc
      841 gcccgcctgg cgatggagct gcacgatgcc ggcgcgcggg tcagcctgat cgcccctaag
      901 aaccatccgg gatgggcgct cgatctgcta tcgcatcgca agacctatcg cgcattgacc
      961 gcaccccgct gccttgaagc cgctttgctg cggtttcggc cggatctcgt cataccctgc
     1021 gatgaaagag ccgtacgtga cctacatcga atttggcggg acacaagcga tccgttcgta
     1081 aagcaactgg ttgaaaagtc gaccgcgccg gccgaaggct ttccgacaat cacctcccgc
     1141 gccgaattgt tgtcccttgc acagcgccac ggggtccgga taccggcttc catgccactc
     1201 gccgatgtca tggcattgga ccaatggaca agcgataacg cggcgccatt tgtactgaaa
     1261 gcggatggaa gctgggccgg cttcggtgtg cgcataatct cgaacgccgc cacggccaag
     1321 gatgccttca ggcggatgac caagcctgtc agcctgcgtt tggcgctccg cgaatcgctg
     1381 cttgaaggca ataacttcga catcagatcg tgtttgcggc gggaacgccg ggcgatgtcg
     1441 gtgcagggct tcgtcgacgg ctggcccgcc aacatcgggg tcgcgtgttg gcagggcgaa
     1501 gttctcgcaa ctatttgtgc cgagtccgtc gcaacaacct cgactaccgg cccgtccaca
     1561 gtcgcccggg tcatcgacaa cgccgaaatg gtcgatgccg ctggacgaat cgtggcggcg
     1621 cttggccttt ccggcatggt cggcttcgat ttcatgatcg aggcggccag cggcgctgcc
     1681 tatttgatcg aaatgaaccc ccggaatacg ccgatctgtg ccgtgcgcct cggcgaggga
     1741 cgcgacctcg ccgaggcctt ggtggcacgt atcgcgaacc ggccgcggcg ccagcggccg
     1801 ccacggacca gtcgtgacat cgtggttttt tttcccgata cgtggcgcga ggatccggcg
     1861 aaccagttcc tgtacagcga gtatcatgac gtgccctggg agcaaccgga gttggtc
//
LOCUS       DASWQN010001255         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1311711, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001255 DASWQN010000000
VERSION     DASWQN010001255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311711"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..648
                     /locus_tag="VGC82_21280"
     CDS             133..648
                     /locus_tag="VGC82_21280"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY0425864.1"
                     /translation="MRRVSLMDRFIDTIEWLAAAFVGIVALDIFISVLLRYFFSVSIP
                     DSYDFGKLLLGILIFWGIAATSYRGSHITVDLVWSACGPFGKRLIDIFATLVLLFVVT
                     IQTIMLFDKVRLTMQDNVLTYDLRIPTWPFALVAWLGDVSAVLLIAIRTWRLIFRPED
                     VADATEPKAVE"
     gene            648..1952
                     /locus_tag="VGC82_21285"
     CDS             648..1952
                     /locus_tag="VGC82_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEY0425865.1"
                     /translation="MEFSSDAVAVTGFVVLFALMLLRVPVGMAMGLVGVSGFGYLVGG
                     GPALKMVGQTSMRIVTDYTFGVIPMFLLMGAIASRTGMSRELFQAANRFVGHWRGGLG
                     IATIAACAGFAAISGSSVATAATFSTVAYPEMRRFGYPKSFATGVIAAGGTLGAMFPP
                     SIVLAVYGLITQQDIGKLFIAGILPGLLAVSMYMITISIIGAARPGWLPATRAASWRE
                     RYQAVREVWAPLLLFVFVIGGLYGGLFTPTEAGGMGAGGAVLISMVRRRLSWADLLAS
                     LLQATRTAAAVFTVLIGAILFGYFLTVTQTPQKVTAFITGLGFSPIGALVLIMLMYLV
                     LGCLMDAMAMVILTVPIIFPAVTALGFDPIWFGIIIVMTVELGLIHPPVGMNVFVIKT
                     VVQDVSFSTIFYGVIPFVLTDILRLAILIAFPAIATWLPGRM"
     gene            2003..2470
                     /locus_tag="VGC82_21290"
     CDS             2003..2470
                     /locus_tag="VGC82_21290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HEY0425866.1"
                     /translation="MWTMSSEALAAEVLGIEVPQGRQLSLIWIMHAVTNGLPISSLDR
                     VFRQVAPDDKAFVYRVVRRPTLARRREAFRTKSRPEQAVLSPDEGSKVARLAKGWAMG
                     REVWGTDDAARAFLFRPHPLLEGQLPIDVVLATEFGPPIVERIVGRLQYGAAA"
BASE COUNT          404 a          729 c          846 g          544 t
ORIGIN      
        1 tgcggaatgg cgcaaggcgg cggaaccgct gacggcgaaa tgggcagcga aagtcgataa
       61 tgccgatgcg gtcatggcgg gcctgaaggc ggcattgaag cagcacggtg cgctcgccga
      121 gtgaccgggc caatgcgccg cgtcagcctg atggaccgct tcatcgacac catcgagtgg
      181 ctggccgcgg cgtttgtcgg gatcgtcgct ctggacatct tcatcagtgt gctgctgcgc
      241 tatttcttca gcgtctcgat ccccgacagc tatgacttcg gcaagctgct gctgggaatc
      301 ctgattttct ggggtatcgc ggcgaccagc taccggggca gccacatcac ggtcgatctg
      361 gtgtggtcgg cgtgcggccc gttcggtaaa cggctgatcg atatcttcgc aacgctggtg
      421 ctgctgttcg tcgtgacgat ccagaccatc atgctgttcg acaaggtccg gctgacgatg
      481 caggacaacg tgctgaccta cgatttgcgc atcccgacct ggcccttcgc gctggtcgcc
      541 tggctgggcg acgtatcggc ggtgctgctg atcgcaatcc gcacctggcg gctgatcttc
      601 cgtcccgagg acgtcgccga cgcaaccgaa cccaaggcag tcgaatagtg gaattttcca
      661 gcgacgcggt ggccgtcacc ggctttgtgg tgctgttcgc cctgatgctg ctgcgtgtgc
      721 cggtcggcat ggcgatgggc ctggtcggtg tcagcgggtt cggctacctg gtgggcggcg
      781 gcccggcgct gaaaatggtc gggcagacca gcatgcggat cgtcaccgac tacacatttg
      841 gcgtgatccc gatgttcctg ctgatgggag caatcgcctc gcgcaccggc atgagccggg
      901 agttgttcca ggccgccaac cgcttcgtcg gccattggcg cggcggcctg gggatcgcga
      961 cgatcgccgc ctgtgccggc ttcgccgcga tcagcgggtc gtcggtggcg accgcggcga
     1021 cgttctcgac agtggcgtat ccggaaatgc ggcggttcgg ctatccgaag tccttcgcca
     1081 ccggggtgat cgcggccggc ggcacactcg gggcgatgtt cccgccgtcc atcgtgctgg
     1141 cggtctatgg gctgatcacg cagcaggaca tcggcaaatt gttcatcgcc ggtatcctgc
     1201 cggggctgct cgcggtcagc atgtacatga tcacgatatc gatcatcggc gcggcgcggc
     1261 ccggctggct gccggccacg agggcggcat cgtggcggga gcgataccag gcggtcaggg
     1321 aagtctgggc gccgctgctg ctgttcgtgt tcgtcatcgg cggattgtat ggcggattgt
     1381 tcacaccgac cgaggccggc ggcatgggcg ccggaggcgc ggtgctgatc agcatggttc
     1441 gtcgtcggct gtcatgggcg gatctgctgg cttcgctgct gcaagccact cgcacggcgg
     1501 cggcagtgtt caccgtgctg atcggcgcga tcctcttcgg gtacttcctg accgtgactc
     1561 agacgccgca gaaggtcacc gcattcatca ccggccttgg tttcagccca atcggcgcgc
     1621 tggtgctgat catgctgatg tacctggtgc tgggttgcct gatggacgcc atggcgatgg
     1681 tgatcctgac ggtgccgatc atctttccgg cggtaacggc gctgggattc gatccgatct
     1741 ggttcggcat catcatcgtc atgacggtcg agctgggcct gatccatcct ccggtcggga
     1801 tgaatgtgtt cgtgatcaag acggtggtgc aggacgtttc attcagtacg atcttctacg
     1861 gagtcattcc gttcgtgctg accgatatcc tgcggctggc gatcctgatt gcgtttccgg
     1921 caatcgcaac gtggctgccc ggccggatgt agctattgca aaacgttact acaagatagt
     1981 gatgtgtatc atgtgagcag acatgtggac tatgtcgagt gaggctcttg ctgctgaagt
     2041 tctcgggatt gaagtgccgc aaggcaggca actgtctctg atatggatca tgcacgcggt
     2101 gacgaatggc ttgccgatct cgtcgctgga ccgcgtcttc cgtcaggttg ctccagacga
     2161 taaagcattc gtttatcgcg tcgtccgcag acccacgctg gcgagacgcc gcgaagcgtt
     2221 caggacgaaa tccagacctg aacaggcagt tctgtccccg gatgagggtt cgaaggttgc
     2281 gcgccttgcc aaaggctggg cgatgggtcg ggaggtatgg gggaccgacg atgcagcccg
     2341 tgctttcttg tttcggccgc acccgctcct tgaaggccaa ctaccgattg atgtggtgct
     2401 ggcgacggaa tttggcccgc ccatcgtcga acggattgtg ggaaggcttc agtacggtgc
     2461 ggctgcgtga cggctcggac gctggatcgc gtcctcacag cctgtcgcat tggcgatccc
     2521 agc
//
LOCUS       DASWQN010001256         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_915529, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001256 DASWQN010000000
VERSION     DASWQN010001256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915529"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VGC82_21295"
     CDS             complement(<1..496)
                     /locus_tag="VGC82_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0425867.1"
                     /translation="MEQLLASLRAAAEPTRLRLLALAARGAFCVMEFTEILGQSQPRL
                     SRHLKLLCDCGLLDRVREGANVWFALPTGDGGTLPRELVARLPADDPVLEADRRQAAR
                     VLAERARVASESFRQQGADWDEMRALDLPAQAVEDALLSLIPAGPDGRLLDIGTGTGR
                     VLELL"
     gene            complement(1078..1866)
                     /locus_tag="VGC82_21300"
     CDS             complement(1078..1866)
                     /locus_tag="VGC82_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0425868.1"
                     /translation="MGIFFAINPLFMLSGFFVGLLVGQTGVGGGSLMTPILVILFGIH
                     PATAVGTDLLYASATKSVGTLVHGLNHTIDWRIVGRLASGSVPATLITLLVVSHYDIA
                     GPTGGRIISVILGVMLLLTAVTLIFRRRFLLVAGPLLDRVTPRQAARLTVLTGVLLGT
                     AVTLSSVGAGALGVTALVMLYPRVPMAVIVGSDIAHAVPLTLVAGVGHWWLGSVDWPL
                     LTSLLSGSIPGIILGSYLSAHVPDAVLRPILATVLCVVGGRLVF"
     gene            complement(2091..>2672)
                     /locus_tag="VGC82_21305"
     CDS             complement(2091..>2672)
                     /locus_tag="VGC82_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEY0425869.1"
                     /translation="VAGPVRVLLPMVTNVAEVRAAREVYERVARCLRERGDRIPEKLP
                     PMGIMIETPGAALSADALALEADFFSIGTNDLTAYTLAVDRSESDVAELYDPMHPAVL
                     RLVQFATEAALRVHMPVSICGELAAAPKLTPLLLGLGVRSLSMNASAVPKVKQVIRSV
                     SIDACVRFAQRVMEQGDSDRIKAMLAGFGRGEG"
BASE COUNT          552 a          873 c          819 g          428 t
ORIGIN      
        1 cgagcaattc cagcacgcgg ccggtgccgg tgccgatatc gagcaaccgt ccgtcggggc
       61 cggccgggat cagcgacagc aaggcatcct cgaccgcttg cgcaggcaga tcgagcgcgc
      121 gcatttcgtc ccaatcggcc ccctgctggc gaaaactttc cgacgcgacg cgggctcgtt
      181 cagcgagcac cctggcagcc tggcggcggt cggcctccag taccggatcg tccgcaggca
      241 gccgcgcgac aagttcccgc ggcagcgtgc cgccatcgcc ggtgggcagg gcaaaccaga
      301 cattagcccc ttccctcacc cgatcgagca aaccgcagtc gcatagcagc ttaaggtgac
      361 gcgacagcct gggctgtgac tggccaagga tttcggtgaa ttccatgaca cagaacgcac
      421 cccgagcggc tagcgccagc aggcgcaaac gggttggttc ggcggcggcg cgcaggctag
      481 caagaagttg ttccatggtc aggatctaat gacataaaga tatgcttatg tctaggttgc
      541 aattcggagc gcacgtttgg ctcgctgacg acagcggacc ggtaaatgtc gcgtgcgggt
      601 gccgcataag ccttcagcga cccgcctcgt cggaggatta caaccagccg gctggaagac
      661 gatcgccgac aggataccat caggcatcac gtggacgacg ttgccgagga agctgaaggc
      721 gaagagctta ggacatggcc gtggcttgcc gggactggct tctcacgcgc ctaggtgtag
      781 gccgcttcca ccatccgcct gacggcttcc tcggccgggt ccggctctgc tggtcgcggt
      841 tccatcttgt cctgcttctg gtcggcgtcg gagacgaacg tcggcaccgg tgggatctcg
      901 gatgcagaag gatggcggcg tggtctggcg gatcgacgta aattgggtat tcgcggcatg
      961 gtcttcgctt ctggcgctca ggctgtggca gggaaccggc cggtgaccac ccgccgggcg
     1021 ggtcgaactg caaggtgccg ttccgaacga tccgtgtcag gatcgtccca atcaaatcta
     1081 aaacaccaga cgccccccga cgacgcaaag cacggtcgcc aggatcggac gcagcaccgc
     1141 atccgggacg tgcgccgaca ggtagctgcc gaggataatt ccggggatcg agccgctcag
     1201 cagcgaggtc agcaacggcc agtccaccga tcccagccac caatgtccaa ccccggccac
     1261 caatgtcagc ggcaccgcgt gggcgatgtc ggagccgacg atgaccgcca tcggcacgcg
     1321 gggatagagc attaccagcg ccgtaacgcc caacgccccc gcaccgacag acgacagcgt
     1381 tacggccgta cccagaagta caccggtcag aacggtcagc ctggccgcct gtcgcggtgt
     1441 taccctgtcc agaaggggac ccgcgacaag cagaaaccgc ctgcgaaaga tcagtgtcac
     1501 cgccgtcagc aacaacatga cccccaggat gaccgagatg atgcgtccgc cggtcggacc
     1561 ggcgatatca tagtgggaga ccaccagcag ggtgatcagc gtagccggca cactgccgga
     1621 tgcgaggcgt cccacgatac gccagtcgat cgtgtggttc aggccatgca ccagcgtgcc
     1681 gacggacttc gtcgccgaag cgtacagcag gtcggtgccg accgctgttg ccggatggat
     1741 gccgaacagg atgaccagga tcggcgtcat caaggagcct ccgcccacgc cggtttgtcc
     1801 caccagaagg ccaacgaaaa acccggaaag catgaacagc gggttaatgg cgaaaaagat
     1861 gcccatgcgg cctcacgtaa tgatccggat tattagcaca ctcattgcgc cggtagcagt
     1921 acgtcgtttt ccacattcga tcgtaatcgt gtctaaacgt cacgaaaccc tgacaagtcg
     1981 aattcgatct ggcggaggtc agcacccaag cggaggccag caccaaaagc ttcagaccga
     2041 gatgccagcc gcggaatgcg ccagttcgcc gggatagcga cgacggagtc ctatccctcc
     2101 ccgcgtccaa atccggccaa catagccttt atccggtcgc tgtccccctg ctccatgaca
     2161 cgctgggcga atcggacgca ggcatcgatc gacaccgacc ggatgacttg cttcaccttc
     2221 ggcacggcgg aggcattcat gctcagcgac cgtacaccca gccccagcag caatggcgtc
     2281 agcttggggg ccgcggccag ttcgccgcag atcgataccg gcatgtgcac gcgcaacgct
     2341 gcctcggtgg cgaattgcac cagtcggagg acggcggggt gcatcgggtc gtacagctcc
     2401 gccacgtcgc tctccgaacg gtccaccgcg agcgtatagg cggtcaggtc gttggttccg
     2461 atcgagaaga agtctgcctc cagtgccagc gcatcagccg acaacgccgc accgggcgtt
     2521 tcgatcatga tccccatcgg cggcaacttt tccggaatac ggtcgccgcg ctcccgcagg
     2581 catcgcgcca cccgttcata aacctcgcgc gctgcccgaa cctcggcaac gttggttacc
     2641 attggcagca acacccgcac cgggcccgcg ac
//
LOCUS       DASWQN010001257         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1706846, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001257 DASWQN010000000
VERSION     DASWQN010001257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706846"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..2006)
                     /locus_tag="VGC82_21310"
     CDS             complement(225..2006)
                     /locus_tag="VGC82_21310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEY0425870.1"
                     /translation="MSNANTAQRNDLPTWDLSDLYSSPDSPQVMADLAKAEQSAKSFG
                     GTWAGTLENAPGAALAAAITEFERIQEMLGRVASYAQLLFAGDSTDPAIGRFYQTVNE
                     RVTAIGSDLIFFTLELNRLDDAVLETRFADPALARYRPWLRDLRVFRPHQLSDELEKL
                     THERDLTANGAWVRLFDETVASMRIDLNGESLTVSDTLNKLSDRDRSVREAAGKAIGK
                     AFGDNIRLFSLITNTLAKDKEIEDTWRHHARPTSSRNRANMVEDEVVDALATAVRDSY
                     PRLSHRYYQMKAKWLGLPKLQHWDRNAPLPNDDDRLIEWPDATKQVLDAYGSFSPELA
                     SVGRRFFDRPWIDARLKPGKAGGAFAHPTVPSVHPYLLLNYHGKTRDVMTLAHELGHG
                     VHQVLAAPQGALMSHTPLTLAETASVFGEMLTFRSLLDTANPAQRRLMLAAKTEDMLN
                     TVVRQVAFYQFETLLHDERRSGELLPERIGELWMKVQTESLGPAFEFTPEYSVFWSYV
                     PHFIHSPFYVYAYAFGDCLVNALYSVFQSGHPGFQAKYLDMLRAGGTKRHKELLAPFG
                     LDASDPAFWNKGLDVISGFIDELEAGG"
     gene            2138..>2739
                     /locus_tag="VGC82_21315"
     CDS             2138..>2739
                     /locus_tag="VGC82_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0425871.1"
                     /translation="MQSYQTWPNLASMMFALARGWPGKPMLRAWRDGNWHSVTWGEFG
                     RMAASCARNLRSAGVCAGDRVMLCMENRPEFPIAETALMAIRAVPVPAYTTNTVDDHA
                     HLLRDSGARAAIVSSGALAERVLAAAAKAGGLDLLVVMAGEAAPEVRHGATIRQWQEL
                     IQDQRPQDDIEAEAAGIPATALACLIYTSGTGGSPRGVMLP"
BASE COUNT          489 a          828 c          890 g          532 t
ORIGIN      
        1 ttgtaatgca gccttagatt tttatggtgc gtttttcttg aattctgagc cgttcgctgc
       61 ggcgaccgca taattgcgca atatggaaag ttggataccg ttggctcaac gaccgcaata
      121 acatgacggc gccgccaagg gctcgcgtcc gctcgtccga agccggcaat acatcgcggt
      181 gtctcgcttg gaaatgatga agcgaccaaa tctggcccgg ccgcctaacc gcccgcctcc
      241 aactcgtcaa tgaatcccga gatcacatcc aggcccttgt tccagaacgc tggatcggac
      301 gcatcaagcc cgaacggcgc cagtagctct ttgtgccgct ttgtcccgcc ggcccgcagc
      361 atgtcgaggt actttgcctg gaaccccgga tgccccgact ggaacaccga gtacagcgcg
      421 ttcaccaggc agtcgccaaa cgcgtaagca tacacgtaaa acggcgagtg tatgaagtgc
      481 ggcacatacg accagaacac gctgtactcc ggcgtgaact cgaacgccgg acccaggctt
      541 tccgtctgca ctttcatcca tagctcgccg atgcgctccg gcagcaactc gcctgagcgg
      601 cgttcgtcgt gcagcagggt ctcgaactgg tagaacgcga cctggcggac gaccgtgttc
      661 agcatgtcct ccgtcttggc agccagcatc aggcggcgct gggctggatt cgcggtatcc
      721 agcagtgacc ggaacgtcag catttcgccg aacacgctcg cggtttccgc cagggtcaac
      781 ggcgtatggg acatcagcgc gccctgcggg gcagcgagca cctggtgtac gccgtgacca
      841 agttcgtgcg ccagtgtcat cacgtcgcgc gtcttgccgt gataattcag cagcaaatac
      901 gggtgcacgc tcggtaccgt cggatgcgcg aaggcgccac ccgccttgcc aggtttcaac
      961 cgggcatcga tccagggcct gtcgaagaag cgtcgtccaa ccgacgcgag ctcggggctg
     1021 aagctgccgt acgcatccag cacctgtttc gtggcatcgg gccactcgat cagccggtcg
     1081 tcgtcgttcg gcagcggcgc attccgatcc caatgctgca gtttgggcaa accgagccac
     1141 ttggctttca tctgatagta gcggtgcgac aggcgcgggt aggagtcgcg aacggcggtc
     1201 gccagtgcat ccacgacctc gtcctcgacc atgttggcgc gattgcggga gctggtcggg
     1261 cgggcgtggt ggcgccaggt gtcctcgatc tccttgtctt tggccagggt gttggtgatc
     1321 agggagaaca agcggatgtt gtcgccgaac gccttaccga tagccttgcc ggcagcctcg
     1381 cgcacggagc gatcgcgatc ggacagtttg ttcagggtgt cgctgacggt cagcgattcg
     1441 ccgttcaggt cgatccgcat ggaagcgacg gtttcgtcga acagccggac ccaggcgccg
     1501 ttcgcggtca ggtcgcgttc gtgcgtcagc ttttccagtt catcggacaa ctggtgtggc
     1561 cggaacacgc gcaggtcgcg cagccagggg cgatagcggg ccaaagcggg gtccgcgaac
     1621 cgggtctcca gcacggcgtc gtccaggcgg ttgagttcca gggtgaagaa gatcaggtcg
     1681 ctgccgatcg cggtcactcg ttcgtttacc gtctgataga aacgtccgat cgccggatcg
     1741 gtggaatcgc cggcgaacag cagttgggcg taggaggcga cgcgcccaag catttcctgg
     1801 atccgctcga attccgtgat cgccgccgct agggcggcgc ctggcgcgtt ctccaaggtt
     1861 cctgcccagg tgccgccaaa ggatttggcg gattgttcgg ctttcgccag atcggccatt
     1921 acctgcggac tgtcgggcga ggaatacaga tcggacaagt cccaggtcgg cagatcattc
     1981 cgctgggcgg tattggcgtt gctcatgtaa gattcgatat ccctgtgaat tgccggattt
     2041 ggtgcgggta gcctgctgcg tcgttttctt ttgtaagcta ggaagggcca acttcaaaga
     2101 acaggctacc ccggctccgc ccggcaccgg aggatacgtg cagagttacc agacatggcc
     2161 caacctggcc tcgatgatgt tcgctttggc ccggggctgg ccaggcaagc cgatgctgcg
     2221 cgcatggcgc gacggcaact ggcactccgt aacgtgggga gagtttggcc gcatggcggc
     2281 gtcctgcgcg cgcaatctgc gatcggcggg tgtctgtgcg ggtgaccggg tcatgctgtg
     2341 tatggaaaac cggcccgagt tcccgatcgc cgaaacggcg ctcatggcga tccgggcggt
     2401 tccggtgcca gcctatacga cgaacaccgt ggatgaccac gcgcatctgt tgcgcgactc
     2461 cggcgcacgg gctgcgatcg tttcgtccgg agcgttggcc gagcgcgtgc tggcggcggc
     2521 ggcgaaggcc ggtggactcg atctgctggt ggtcatggcc ggcgaggccg ctcccgaagt
     2581 caggcatggg gcgacgatca ggcaatggca ggaactgatc caggatcagc ggccgcagga
     2641 tgatatcgaa gccgaagcgg ccggcattcc cgcgaccgcg ctggcctgcc tgatctatac
     2701 ctcgggcacc ggcggttctc cacggggcgt gatgctgcc
//
LOCUS       DASWQN010001258         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1443964, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001258 DASWQN010000000
VERSION     DASWQN010001258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443964"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="VGC82_21320"
     CDS             <1..1462
                     /locus_tag="VGC82_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEY0425872.1"
                     /translation="DFVEFTHGCRQRGIRVIIDLVVNHTSDQHPWFREARRSKDSPYR
                     DWYVWADKKPANADKGVVFPGVQKSTWTYDKEARAWYFHRFYDFQPDLNTSNPHVQAE
                     ILKIMGAWIQLGVSGFRMDAVPFVIATKGPKVRTPVEQYDMLRSFREFLQWRQGNAII
                     LGEANVVPKTDMEYFGKDGDRMPMMFNFHVNQHLFHAMASADSRPLAKALMETRPRPA
                     TAQWGQFLRNHDELDLGRLTKAQRDLVFERFGPDKDMQLYDRGIRRRLAPMLGGDRRR
                     IELAYSLLYTMPGTPVIRYGDEIGMGDNLDLPERNCARTPMQWSTEPHAGFTESDKPV
                     LPVIDKGPYGYEHVNVAKQRRDPNSLVNWTERLIRMRKEVPEVGWGDFKVIATRDPAV
                     LIIRYDWRNNSVLFVHNLDEKPREISFATGLAGEAGKLLVNLLSEDHSQADKRGKHNL
                     LLEAYGYRWYRVGGLDYLLKRSDIDADVSGKSSHPA"
     gene            complement(1599..>1824)
                     /locus_tag="VGC82_21325"
     CDS             complement(1599..>1824)
                     /locus_tag="VGC82_21325"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEY0425873.1"
                     /translation="DYVPIAAQGDLLLYRRRGGQGSAVVALNLGAEPVSLALDALDGE
                     ILLSTLMDRQGEKLHGALDLRDNEGVIIGE"
BASE COUNT          373 a          590 c          545 g          316 t
ORIGIN      
        1 cgactttgtc gaattcaccc atggctgccg gcaacgcggc atccgcgtca tcatcgatct
       61 cgtcgtcaat cacacctcgg accagcaccc atggtttcgc gaagcacgcc gctcgaagga
      121 ctcgccctac cgcgactggt atgtctgggc cgacaagaag cccgccaatg ccgacaaggg
      181 cgtggtgttt ccgggtgtgc aaaaatccac atggacctac gacaaggaag cccgcgcctg
      241 gtattttcac cgcttctatg acttccagcc cgacctcaac acctcgaacc cgcacgtgca
      301 ggccgagatc ctcaagatca tgggggcctg gatccagctc ggcgtgtccg gattccggat
      361 ggatgcggtg ccgttcgtga tcgccaccaa ggggccgaag gtccgcaccc ccgtcgagca
      421 atatgacatg ctgcggtcgt tccgggaatt cctgcagtgg cggcaaggca acgccatcat
      481 tctcggcgaa gccaatgtgg tgcccaaaac cgatatggaa tatttcggca aggacggcga
      541 ccgcatgccg atgatgttca attttcacgt caaccagcat ctgttccatg cgatggcgtc
      601 ggccgattcc cgtccgctgg cgaaggcgct gatggagacc aggccgcggc ccgcgaccgc
      661 gcaatggggc caattcctgc gcaaccatga cgaactcgac ctcggccggc tgaccaaggc
      721 gcagcgcgac ctggtgttcg aaagattcgg cccagacaag gacatgcagc tgtacgaccg
      781 tggcatccgg cgccggctgg cgccgatgct gggcggcgac cggcggcgca tcgaactggc
      841 ctacagcctg ctgtacacga tgccgggaac gccggtgatc cgttatggcg acgaaatcgg
      901 catgggcgac aatctcgatc tgcccgagcg caattgcgcg cgcacgccga tgcagtggtc
      961 gaccgaaccg cacgccggct tcaccgagag tgacaaaccg gtgctgccgg tgatcgacaa
     1021 ggggccttac ggctacgagc acgtcaacgt cgccaagcag cgccgcgatc ccaactccct
     1081 ggtgaactgg accgagcgcc tgatccggat gcgcaaggaa gtccccgagg tcggctgggg
     1141 cgacttcaag gtgatcgcaa cgcgcgatcc ggcggtgctg atcatccggt acgactggcg
     1201 caacaattcg gtattgttcg tgcataatct cgacgagaag ccgcgcgaaa tatcattcgc
     1261 gacaggcctt gccggggaag ccggcaagct tctggtcaac ctgctgagcg aggaccacag
     1321 ccaggccgac aagcgcggca agcacaattt gctgctggaa gcctacggct atcgctggta
     1381 ccgcgtcggc ggcctcgact atctgctcaa gcgcagcgac atcgacgccg acgtgtcagg
     1441 caagagcagt catccggcgt gagaaaattt atccggtcgt ccccgcgaac gcggggaccc
     1501 atagccgctg acgttggcta ttgcatgagc ggctccggcg tcctgcaaat caaacatcgg
     1561 tgtttatggg tccctgcttt cgcagggacg acagcgagct attcgccgat gatcacgcct
     1621 tcattgtcgc gcaaatccag cgcgccgtgc agcttctcgc cttgccggtc catcaaggtg
     1681 gagagcagga tttccccgtc gagcgcatcc aatgccagcg aaaccggctc cgctcccaga
     1741 ttgagcgcga ccaccgccga accctggcct ccccgcctgc ggtacagcag caagtcgccc
     1801 tgtgccgcaa tcggaacgta atcg
//
LOCUS       DASWQN010001259         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1839593, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001259 DASWQN010000000
VERSION     DASWQN010001259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839593"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VGC82_21330"
     CDS             <1..1111
                     /locus_tag="VGC82_21330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0425874.1"
                     /translation="FERIALEGPILDQPVPATLDDISTILYTSGTTGRPKGAIITHGM
                     NFWNTVHSMSLAGIARSTVFLGLLPLFHTGGLNVFSYPVFQVGGTVLIMRSFDPGEAL
                     RLIGDPSVGVTHMFGVPANYQFMGQHPRFQETDMGRLVFAGVGGAPTPDTILRTWQEQ
                     GAILQQGYGMTETSPLVLVLDKEDAVRKAGSAGKPALNMEMRVVGDDGTDAAFGMIGE
                     LWVKGPNITKGYWNQPEVTAASFTDGWLHTGDAALVDDEGYYFIVDRWKDMYISGGEN
                     VYPAEIENVIYQNELVAEAAVIGISDARWGQVGRAIVVVKAGHTLTEDAVVAHCAANL
                     ARYKLPHSVVFAEALPRNATGKVHKPTLRQAFGEG"
     gene            complement(1401..2576)
                     /locus_tag="VGC82_21335"
     CDS             complement(1401..2576)
                     /locus_tag="VGC82_21335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEY0425875.1"
                     /translation="MLNSTNRILTTHTGSLPRPPALTELYARRAEGEAIDEIELDAAG
                     KAATRHVVRQQIASGIDVGNNGEQQREAFFLYVRRRMSGFGGSWIRRPMADIVKYPGY
                     RAWAAEQDAVRRSVSNRGALPKAIGAVIYLDPALVKAECDEFLATLSEVEGGFAEPFM
                     TAPSPGIIAAALQNEHYDTEDAYLHALGQALRVEYEAIVGAGFLLQIDAPDLALERHV
                     SFQDRPLGDFLDFAERVVGTINQALVHVPRDRVRIHACWGNYEAPHDCDVDLHDILPT
                     ISQAKVGGWVLPFANPRHAHEYRCLKNLPLGEGQVVVAGVIDPLTNFVEHPEVVADRL
                     ERVVQVVGDPTRVLAGTDCGFDTSAGRGRVAEDVVWAKLRAMSDGARIASKRLGMRG"
     gene            complement(2576..>3115)
                     /locus_tag="VGC82_21340"
     CDS             complement(2576..>3115)
                     /locus_tag="VGC82_21340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY0425876.1"
                     /translation="GGGSNRVGALGYVSCAQELMQQADEMGLRIDRIVTATGSAGTHA
                     GLVVGLEGMNAQVPVLGIGVRLPRERQEENVYRLALATAEYVGMKSGVFREAVVANCD
                     YVGEGYGIPTEGMGEAVRMMAQEEGILLDPVYSGKAMAGMIDLIRRRVLPKDETIVFL
                     HTGGAVGLFGYTPFLEGAV"
BASE COUNT          562 a         1021 c          954 g          578 t
ORIGIN      
        1 gttcgagcgg atcgcgctgg aaggtccgat cctcgatcag ccggtgccgg cgacgctgga
       61 cgatatttcg accattcttt acacatcagg aacgacaggc cggcctaagg gcgcgatcat
      121 cacccacggc atgaacttct ggaacaccgt gcacagcatg agcttggctg ggatcgcacg
      181 ctccacggtg ttcctcggct tgttgccact gttccacacc ggcggattga atgttttctc
      241 ctacccggtg ttccaggtcg gcggaacggt gctgatcatg cgcagcttcg acccgggcga
      301 ggcgctgcgg ctgatcggcg atccgtcagt cggtgttacg cacatgttcg gcgtgccggc
      361 gaactatcag ttcatggggc agcatccgcg tttccaggaa accgacatgg gccggttggt
      421 cttcgccggc gtcggcggcg ccccgacgcc tgatacgatc ctgcgcacct ggcaggagca
      481 aggtgccatc ctgcagcagg gctacggcat gaccgagacc agcccgctgg ttctggtgct
      541 ggacaaggag gacgcggttc gcaaggctgg ttcagccggc aaaccggcgc tgaacatgga
      601 gatgcgcgtg gtcggcgatg atggcaccga cgctgcgttc ggcatgatcg gcgagctgtg
      661 ggtgaaaggt ccgaacatca ccaagggtta ctggaaccag ccggaggtca ccgcggcttc
      721 gtttaccgat ggctggctgc ataccggcga cgcggccttg gttgacgacg agggctacta
      781 cttcattgtg gaccggtgga aggacatgta catctctggc ggtgagaatg tttatccggc
      841 ggagatcgag aacgtaattt atcagaatga attggtcgcc gaggccgcgg tgattggaat
      901 ttccgacgcg cgctgggggc aggtcggacg cgcgatcgtt gtcgtaaagg cggggcacac
      961 gctgaccgag gatgcggtgg tcgcgcattg cgcggccaat ctggcccgct acaagctgcc
     1021 gcattcggtg gtctttgccg aagcgctgcc tcgtaatgcg actggcaagg tgcacaaacc
     1081 gacgttgcgg caagcttttg gcgaaggttg agggcggaga gaagggcatg cctcgccttc
     1141 atgaccaggc atacatgctt cgcttccatt ggatccgcca gatcgcggcg gccttggtgg
     1201 gccttcagct gcacttgctt atccgggcat aaaccagttc gtgacaaaga actggccgtg
     1261 acctcctcgc gttcggcggt tcgacgacgc gccaggccga agcgttcagg cgcgaccatt
     1321 gggccggcgg cggagaggcg tcacgcccat ttcgagcaac gcgccagccg gtctgcggca
     1381 ggctctcgac gacacggggc tcaaccccgc atccccagcc gtttggacgc gatccgcgca
     1441 ccgtcggaca tggctcgcag cttcgcccag acgacatcct ccgccacgcg gccgcgtccg
     1501 gcggacgtgt cgaacccgca atccgtcccg gccaaaacgc gggtcggatc gcccaccact
     1561 tgcaccacgc gttccagccg gtccgccacc acctcggggt gttccacaaa gttggtcagc
     1621 ggatcgatca cgccggcgac cactacctgc ccttctccca acggcaggtt cttcaggcat
     1681 cggtactcat gcgcgtgccg tgggttggcg aacggcaata cccatccccc aaccttggcc
     1741 tggctgatcg ttggcagaat atcgtgcaga tcgacgtcgc aatcgtgcgg tgcctcgtaa
     1801 ttcccccagc aggcgtggat acgcacccgg tcgcgcggaa cgtgtaccag cgcctggttg
     1861 atcgtcccga cgacacgttc ggcgaaatcg agaaagtcac ccagcggccg atcctggaat
     1921 gagacgtgcc gctccagcgc aaggtccggg gcgtcgatct gcaacaggaa tcccgcgccc
     1981 acgatcgcct catactccac gcgcaatgcc tggccgagtg cgtgcaggta agcgtcttcg
     2041 gtatcgtaat gttcgttctg caacgccgcg gcgataatgc cgggggacgg cgcagtcatg
     2101 aacggttcgg caaaaccacc ctcgacctcg gacagcgtcg cgaggaactc atcgcactca
     2161 gccttcacca gcgcggggtc cagatagatc accgcgccga tcgccttcgg caaagctccg
     2221 cgattgctca ccgatcgccg cacagcgtcc tgttccgccg cccaggcccg gtaacccgga
     2281 tatttcacga tgtcggccat cggccggcgg atccagctcc cgccgaaccc gctcatgcgc
     2341 cggcggacat agaggaaaaa cgcctcccgc tgctgctcgc cgttgttgcc gacatcgatg
     2401 ccggaggcga tctgctgccg cacgacgtgg cgggtcgccg ccttgccggc ggcatcgagt
     2461 tcgatttcgt caatagcttc gccctctgcc cgccgtgcgt acagttccgt caaggccggc
     2521 ggacgcggaa ggcttccggt gtgcgtcgtc aggatgcggt tcgtgctgtt cagcatcaga
     2581 ccgccccttc cagaaatggc gtatacccaa acaatcccac cgcgccgcca gtgtgcagga
     2641 agacgatggt ctcgtccttg ggcaggaccc tgcggcggat caggtcgatc attccggcca
     2701 tcgccttccc agaatagacc ggatctagaa ggatcccctc ctcctgcgcc atcatgcgaa
     2761 ccgcttcgcc catgccctcg gttggaatgc cgtagccctc gccaacatag tcgcagtttg
     2821 caacgacagc ttcccggaaa acgccgctct tcattccgac atattccgcc gtcgcgagcg
     2881 ccaggcgata aacgttttct tcctgtcgtt cgcgcggcag tcgcactccg atgccaagta
     2941 cgggcacctg agcgttcatt ccctcaaggc ccaccaccag tccggcgtgg gtcccggcac
     3001 taccggtcgc ggtcacgata cggtcgatcc gcagccccat ctcgtcggcc tgctgcatta
     3061 gctcctgcgc gcaggagaca taaccgagcg cgccgacccg gttcgatccg ccgcc
//
LOCUS       DASWQN010001260         3948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2235531, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001260 DASWQN010000000
VERSION     DASWQN010001260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2235531"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VGC82_21345"
     CDS             <1..1404
                     /locus_tag="VGC82_21345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425877.1"
                     /translation="GSVVVLMTIDWRLAAALLVAVLGLTALLRRVGARGFPVHRAYHA
                     QSAEVLGALSDVLSNISLVHAFDSRHRESERLRADLSVESRAHARSWSYLERTRCLHD
                     VSFWLVTAGLLTGSIMVWHSGRITTGDVVVASTLALRVLSGSRELALSLLGLADQLSG
                     LSESIEVLCAPPDIVETGSTPRLYRCRGPIEFRGVTLSQEGAPALFREFDLYIPEGQR
                     VGIVGPSGAGKSTLFRLLQRQAEPQSGSVWIGGQRVDAVTRQSLAAVFAVVTQEVALL
                     HRSVLENLRYARPDASDEEVHAVAVASGCDSFIRALPRQYDSLVGERGVRLSGGQRQR
                     IAIARAMLKNAPIVLLDEATSALDTEAELDVQAGLENLTRGRTVLAIAHRLSTIMSFD
                     RVIVLQNGRIVEDGPPGILSHGIGPFGRMWRLQERANARPAQQGMDLAWREVIYAAGP
                     DNAAFATVPRQPGRATG"
     gene            1778..2062
                     /locus_tag="VGC82_21350"
     CDS             1778..2062
                     /locus_tag="VGC82_21350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425878.1"
                     /translation="MLSQDHPGKGSDMAEQETAVLIAAIKSALSTIPEVHRKAALKHA
                     SEAVWAELIDQSEHAKAASRGAPPELGAKAAAATAAIQELAGHTTNPKRD"
     gene            complement(2162..2392)
                     /locus_tag="VGC82_21355"
     CDS             complement(2162..2392)
                     /locus_tag="VGC82_21355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425879.1"
                     /translation="MVASHEKPTDANANLPQGDETERASGGRTDADKAADAAPKDRAQ
                     DENLKQRQEDLLDEAIEETFPASDPIAPKQIT"
     gene            2912..3463
                     /locus_tag="VGC82_21360"
     CDS             2912..3463
                     /locus_tag="VGC82_21360"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY0425880.1"
                     /translation="MMTNFSRFGLTALLFLAACQRAEQAATTAQSAVQAQTTPTLSTS
                     DATFLNLAGTSGIEEVTFGQLAQTKASRAAVRNFAAQMVADHTTANQQLVALARSKQL
                     TPPSSMDLMHDQLFQKLQGLRGSAFDRAYMDSQVQDHAMVVEAFQTEAKNGTDPQVRS
                     FAQQNLPTMQQHLQMARSLTSQH"
BASE COUNT          703 a         1239 c         1266 g          740 t
ORIGIN      
        1 ggatcggtgg ttgtgctgat gacgatcgat tggcgcctcg ccgccgccct gttggtggcc
       61 gtccttggct tgaccgcgct gctgcgcagg gttggcgccc gcggcttccc ggtccaccgc
      121 gcctaccacg cgcagagcgc cgaggtcctg ggcgcgcttt ccgatgtgct gtccaacatc
      181 tccctggtgc atgccttcga cagccgccac cgcgaaagcg agcgcctgcg ggccgatctt
      241 tcggtagaat cgcgcgccca tgcgcgaagc tggagctatc tggagcgtac gcggtgcctt
      301 cacgatgtct cgttctggct ggtcacggcc ggcctgctga cgggatcgat catggtctgg
      361 cacagcggac gaatcacgac gggcgatgtc gtggtcgcca gcacacttgc gctccgcgtg
      421 ctgagcggtt cgcgggagct ggcgctgtcg ctcctcggcc tggcggacca actgagcggc
      481 ttgtccgagt cgatcgaggt cctttgcgca ccacccgaca ttgtagaaac gggctcaacg
      541 ccgcgcctgt accgttgtcg cggtccgatc gagttccgcg gcgtcaccct gtcgcaggaa
      601 ggcgctcccg cgctgtttcg cgagttcgat ttatatatcc cggaaggcca gcgcgtgggg
      661 atcgtcggtc cgtccggtgc cggcaagtcc acgctgttcc ggctgctcca gcggcaggct
      721 gaaccacaaa gcggaagtgt atggatcggc ggccagcgcg tcgatgcggt gacgcgccag
      781 agtctggcgg ctgttttcgc cgtcgtgacg caggaagtcg cgttgctgca ccggtcggtg
      841 ctggagaatc tgcgctacgc gcggcctgat gcatcggatg aggaggtgca tgcggtggcc
      901 gtggcttccg gatgcgacag ctttatccgg gcactgccgc gccagtacga cagcctcgtc
      961 ggcgaacgcg gcgtgcggct ttcaggtggg cagcggcagc gtatcgccat cgcccgtgcg
     1021 atgctgaaga atgcgccgat cgtgttgctg gacgaagcga cttccgcact ggacacggaa
     1081 gcagaactcg acgtgcaggc aggtctggag aacctgaccc gaggccgcac ggttcttgcc
     1141 attgctcatc gactgtccac catcatgagt ttcgaccgag tgatcgtgct gcaaaacggg
     1201 cggatcgtgg aagatggccc gccgggcata ctgtctcacg gcatcgggcc cttcggccgc
     1261 atgtggcgtc tccaggaacg tgccaatgct cggcccgcgc aacagggcat ggatctcgcc
     1321 tggcgcgagg tgatctatgc agccggcccg gacaatgcgg cgttcgccac cgtgcctcgg
     1381 caaccgggca gagcgacggg gtagatcggc gctggccttc gttgtcgccc gtagtcgtcc
     1441 tatgaagccg gcggatcacg gcgcccggca ttggcattgt ccccgaatgc cgctgatggg
     1501 atccgaacgg caccaatcgc ctcgccgaat ccccgtgttg cacatgaccg catcgatcaa
     1561 ttctgccttg cctggttttt cgccgatcgc ccctgcacca aggacgagcg ggccaatcgg
     1621 agcgatcatg tcgcccgccc ttgcctcacg ttccagtaac agatgatacg cgggttcgcc
     1681 gcacgcaggc ctgtgcgcct gacgaagtcc gctgtgcgac ctgaacactg gcggtcggca
     1741 tcgaagactt cggcaactcc gttcggaagc cggcgttatg ctgtcccagg atcaccctgg
     1801 caaaggttcg gacatggcag agcaggaaac cgccgtactg atcgcagcaa tcaaatcggc
     1861 actttccacc attccggagg tgcatcgcaa agcggccttg aaacacgcca gcgaggcggt
     1921 ttgggcagag cttatcgacc agtcggagca tgcgaaagcc gcctcgcgag gggccccgcc
     1981 cgaactgggg gccaaggcgg cggcagcgac ggctgccatt caggaactcg cggggcatac
     2041 gacgaaccca aagcgggatt aagctcgtaa aggccggcgt tctacccgac ccacggctgt
     2101 cgttcggaag ctcgggtgtc gttcggaagg ccgggggatc cgcgggtgca aaagttagcc
     2161 gtcaggtaat ctgcttcggc gcgatcggat cactggccgg aaaggtttcc tcgatggcct
     2221 cgtccagcag gtcttcttgc cgctgcttca ggttctcgtc ctgggcgcgg tccttgggcg
     2281 ccgcgtctgc ggccttgtca gcgtccgttc ggccgccact ggcgcgctcg gtctcatcgc
     2341 cctggggcag gttggcgtta gcatcggtgg gcttttcatg ggaagctacc ataagtctct
     2401 ccgagaggtg ttcatctttt ggataaaggc tccgtttcgg aacgaccgct tcggtcgcga
     2461 tctccagttc cgttacggca ggcgcgggcc catgggcgct gcaacaaggc cgcggcgccc
     2521 gggatcgagg ccgcagatgt agagaatgtc tggccatgct cggcgcggcc gtccacgctt
     2581 tcccctgaac gcgcgaaagt cgcggataac cggcacaagg cctatcgtga cgccggtggc
     2641 gcagaccgtg ccatgacggc aaagcctgcc ggttcttcat gcggtcgtcc ttcacccatc
     2701 aatgccaaca gcgtggccgc cagcagcggg tttcggggtc acgacaaaga catgcccaga
     2761 aggcggccgt gaaccaccct gccgcacccg cccctaacgc tggaagccga tggtttcctg
     2821 ccacccggga cacatttgcg actgcggcca accatggttc tcgagatgcg ttgcggcatg
     2881 agccgtattg ttgcgtcgca atggagccaa catgatgacg aatttctctc gcttcgggct
     2941 tactgccctg ttgttcctcg ccgcgtgtca acgcgcggag caggcggcca ccaccgcaca
     3001 gtcggcagtg caggcgcaga cgacgccaac gctgtcaacg tccgacgcta ccttcctcaa
     3061 cctggccggc acatccggaa tagaggaggt cacgtttggt cagcttgcgc agacaaaggc
     3121 cagtcgagcc gccgtacgga atttcgccgc tcagatggtc gctgaccata cgacggcgaa
     3181 ccagcagctt gttgcgctgg cgcgaagcaa gcagctgacg ccgccttcca gtatggatct
     3241 gatgcacgat cagttattcc aaaagctgca ggggctgcgt ggatcggcgt tcgatcgcgc
     3301 ctatatggac agtcaggtgc aggaccatgc catggtcgtc gaggcattcc agaccgaagc
     3361 caaaaacggg accgatccac aggtccggag cttcgcgcag cagaaccttc caaccatgca
     3421 gcagcacctc cagatggcac gtagtctgac aagtcagcat tgatcgagcg ctgaaggctg
     3481 acgccgtcgc aagatgccgt ccgtcgagcg acggccattt cgggctcagg ctgatgccgg
     3541 tgaatgcgga tcgcttcgat ccttccgagg caaagatgcg gcacttgccg tgcaacaccg
     3601 gcctggaccc aggtcgtaat gcatcctgta tcctagggtc ccgatcacct atgacaaaca
     3661 tgccgctggc tgtggaatcc gtagccgcgt cggctgtaag tcgttccacc agcaagccca
     3721 gaatctgcgt ctagtgtgcc cagactctgt gtgtgtaccc agactgggtg gctacgtcac
     3781 gatcaggacg atcggccgcc gctgggtacc tcgcatataa cgcctgaacg cggatcacgt
     3841 gtgacggagc ggccaggatc agcggaaagc ctgccaccga ctggggttta tgccgcatgg
     3901 atcagaacca gcagcaggga cggccgcgga tcgtggtcat cggaggag
//
LOCUS       DASWQN010001261         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1707319, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001261 DASWQN010000000
VERSION     DASWQN010001261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707319"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VGC82_21365"
     CDS             <1..180
                     /locus_tag="VGC82_21365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425881.1"
                     /translation="VRSRSTAAEAEGAWATVIPSLLQGAFGPPDGGGANKEDFSGGAA
                     GAGILPMTPPSGALD"
     gene            complement(316..1863)
                     /locus_tag="VGC82_21370"
     CDS             complement(316..1863)
                     /locus_tag="VGC82_21370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide chain length
                     determinant"
                     /protein_id="HEY0425882.1"
                     /translation="MLSTYLRAAWRRRWMGVMVAWMVCGVGWVGVYTVPNQFESAARL
                     FVDADAVLTPLLRGLAADSAPTTQLEILQRTLLSRPNLEKLVSKTDLDLTLNSPSDRE
                     RLLTRLSTEIKVNPQTKNLFTIVYRDKSPKLAHDVVQTLLTIFVESATGGSRSDMENA
                     RRFLERQIQSYEQQLRAAEKRRADFRARYIEILPADNNPNVPALESARAGAQALEGKL
                     QDMLISRDALKQEVENTPPMLVVESGSNGLPYGFPVGPVPKSRLQEAQEQLSMLLLKD
                     TEQHPDVVAQRKLIESLKNAPREASGAKETSGGRDNASTLKRSVPNQVYDQLKVRLID
                     ADTMVISLQRQRDEAVRYRDRLEKVQREQPGLIAEYQNMDRDYTVLRKNYEELLGRLQ
                     SANIAQAADTQADKVKLQVIDPPEIPRLPVAPNRMLLITGVLLGGLSTGAGLTVLFGQ
                     LDSSFSTVEGLRQLGLPVLGGISVLGLIPFRQRLLTVLRFSAAIAILVGVYGGLVVNI
                     LRSAALI"
BASE COUNT          370 a          609 c          619 g          377 t
ORIGIN      
        1 gttcgctcca ggtccacggc agccgaggcg gaaggcgctt gggctacagt gatcccttcg
       61 ttgctgcagg gtgcgttcgg gccgccggac gggggcggcg cgaacaagga agacttctcc
      121 ggcggtgccg ccggcgctgg catcttgcca atgacgccgc cttcgggtgc gttggattga
      181 cgaagaagct gggccgccga agcgtccagg ccgccggatt gcgacaagcg ttcggcaacg
      241 cgttcgatca gatgggatgc cttgggtgtc aacatagacc gccagcttcg cccgatgggt
      301 gcttgtaggt ggccactaga tcaaagccgc cgaccgcaga atgttgacca cgagaccgcc
      361 atacacgccg acgaggatcg cgatggcggc gctgaagcga agcacggtca gcagccgttg
      421 gcggaacggg atcaagccga ggaccgaaat gccgcccagc accggcagac caagctggcg
      481 caacccctcg acagtggaga acgaactgtc caactgcccg aacagcacgg tcagtcctgc
      541 gccagtcgaa agtccgccca gcaacacgcc ggttatcagc agcatccggt tcggtgccac
      601 cggcaaacgg gggatctctg gcggatcgat gacctgcagc ttgaccttgt cggcctgtgt
      661 gtccgccgcc tgagcgatat tcgcggactg cagccggccc agcaactcct catagttctt
      721 ccgcagaacg gtgtaatcgc gatccatgtt ctgatattcc gcgatcaatc cgggctgctc
      781 ccgctggacc ttttccagcc ggtcgcggta acgcaccgcc tcgtcgcgct gtcgctgaag
      841 cgaaatcacc atggtatcgg cgtcgatcag cctgaccttc aactggtcgt aaacctggtt
      901 gggcaccgaa cgtttgagcg tgctcgcgtt gtcccgtccg ccggaggttt ccttggctcc
      961 ggaggcttcg cgcggcgcat tcttcagcga ttcgatcagc ttgcgctgag ccacgacgtc
     1021 cggatgctgt tcggtatcct tcagcaacag catgctgagt tgttcctggg cttcctgcag
     1081 ccgcgacttc ggtactggcc cgaccgggaa tccatagggc aggccgttcg agccgctctc
     1141 gacaaccagc atgggcggcg tgttctcgac ctcctgtttc aaggcgtcgc gggaaatcag
     1201 catgtcctgc aacttgccct ccagcgcctg agccccggcg cgcgccgatt ccaatgccgg
     1261 aacgttggga ttgttatcgg ccggcaggat ctcgatatag cgggcgcgga aatcggccct
     1321 gcgcttctcc gcggcgcgaa gctgctgctc gtaggattgg atctgccgtt ccaggaagcg
     1381 gcgggcattt tccatgtccg aacgcgaccc gccggtcgca ctttcaacga aaatggtcag
     1441 cagggtctgc accacatcat gcgccagctt ggggcttttg tcccgataga cgatcgtgaa
     1501 caggttcttg gtctggggat tgaccttgat ttcagtgctg aggcgagtca gcagccgctc
     1561 gcggtcggac ggactgttca gggtcagatc gaggtcggtc ttcgaaacca gcttttccag
     1621 attcggccgg ctaagcaacg tgcgctgaag aatttcgagt tgcgtggtcg gtgcagaatc
     1681 cgcggcgagg ccgcgtaaca gcggcgtcag gacggcgtcc gcgtcaacga acaggcgggc
     1741 ggccgattcg aactggttgg ggacggtata gacgccgacc cagccaacgc cgcaaaccat
     1801 ccaggcaacc atcacgccca tccagcgacg gcgccaggct gcgcgaagat acgtggaaag
     1861 catgacacgc aaggcatcca taaccggctg ttccctagaa ccaggattgc ggaacgatca
     1921 gggtgtcgcc tgcattcatc tcgatgttct ggccgatgtc gccgtccttg atcag
//
LOCUS       DASWQN010001262         3365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1312399, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001262 DASWQN010000000
VERSION     DASWQN010001262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312399"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..1321
                     /locus_tag="VGC82_21375"
     CDS             383..1321
                     /locus_tag="VGC82_21375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0425883.1"
                     /translation="MLIFALRRLLQAIPILFGVSLVVFGLVHIVPGNPIDMLMPPEAS
                     PEVIAQMKAAFGFDKPLYMQYLLWLMRAVQGDFGLSVFNATPVWGQLIAALQNTLVLA
                     VLGAALGFSMGILCGMVSALYHGRWPDKVFSAIATVGVSLPHYWCAIVLVLMCAVLHD
                     WLPAQGMGDDSLPLYERLQYLVLPVITLSLIPMGVIGRLVRATVLDILGQDFVGALSA
                     KGLTRWPVIRHIAKNAAPPVLALMGLQFGYLLGGSILVETVFNWPGSGNLLNLAIFRR
                     DIPVLQATILVLATFFVLLNLLVDIAQAAIDPRIRR"
     gene            1335..2240
                     /locus_tag="VGC82_21380"
     CDS             1335..2240
                     /locus_tag="VGC82_21380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0425884.1"
                     /translation="MSDITLDTRHAAQADAPAAPAPGYWKSVSQRLLRDKTTVVVTLM
                     LLGILFITLGAPLVTDYDPYQGNVLSRLKPIGTAGHWLGTDETGRDLWTRLCYGGRLS
                     LLAAILPVTISLFVGGFLGILAGYAGGIAGTLIMRTMDVFYAFPSILLAIAICGLLGN
                     GLSNSIIALAVVFIPPMVRVSETVTTQARNLDFVEAARASGTTTMQIIRHHVLANVLG
                     PILVYATSLISVSIILSAGLSFLGLGVTPPQPEWGLMLNSLRQAIYVQPYVAALPGLM
                     IFLTSMCFNLMSDGFRSAMDVRLNK"
     gene            2237..3292
                     /locus_tag="VGC82_21385"
     CDS             2237..3292
                     /locus_tag="VGC82_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425885.1"
                     /translation="MSAALRQDPRDRGGPAQPLLIVENLTKHFPVRGGLLNRQVATVQ
                     AVDDVSFSVRKGETLGIVGESGCGKSTTARLLMRLMVPDAGSVVFDGDAVGEKRGISV
                     SEMRRQMQMVFQDSYTSLNPRMTMAESIAYAPRMHGLARKDAYDRAESLLSRVGLNPR
                     QYARRYPHELSGGQRQRVNIARALALEPRLVILDEAVAALDKSVEAQVLNLLGDLKQS
                     LNLTYVFISHDLNVVEYMSDRVMVMYLGRVVEAGSVESIYRHPRHPYTRALFASKPTL
                     DPDRRTTEMPLTGDPPNPINPPSGCRFRTRCVFAQPRCAETVPPLAAPFDGDADPTHV
                     VACHIPEAQSAKAVHHA"
BASE COUNT          563 a         1119 c         1025 g          658 t
ORIGIN      
        1 cgacgatgtc ccttgccccg gcgatctccg ccaccgcccc aggagccatg aacccgttct
       61 gtaaatcgac tacgatatgg gcagtccgct tacaatcgag gctgtcgaac acgcgaacgc
      121 ggcctctcct ctgtacaatg cggtcaatta cggcaggatt gatgacgctc gtatgcatgg
      181 cctctcctga tatgcctgtt tgttagactg aaccttccgt acgaggctaa atcggcaggc
      241 gatttaatgc aatcgctcaa aattcgctca aatacccctg gacagcgatg caggctgtgc
      301 taggagttat tggattgctg caatgcaaca tattcccgcg caccatgagc gggcccaacc
      361 caggagcggg aaacaggatc gcatgctcat cttcgccctc cgccgcctgc tgcaggccat
      421 tcctattctt ttcggcgttt ccctcgtggt cttcgggctg gtgcacatcg tgcccggcaa
      481 cccgatcgac atgctgatgc cccccgaagc ctcgcccgag gtcatcgctc aaatgaaggc
      541 ggcgttcggc ttcgacaagc cgctctacat gcagtacctg ctctggttga tgcgggcggt
      601 gcagggcgac ttcggcctgt cggtgttcaa tgccaccccg gtatggggcc aactgatcgc
      661 cgcgctgcaa aacaccctcg tcctcgccgt cctgggcgcg gcgctcggtt tttccatggg
      721 catcctctgc ggcatggtct ccgcccttta tcacggccgt tggccggaca aggtgttctc
      781 cgccatcgcc accgtcgggg tttcgctgcc gcactactgg tgcgcgatcg tgctggtgct
      841 gatgtgcgcc gtgctgcacg actggctccc ggcccagggc atgggagacg actcgctgcc
      901 cctgtacgag cgactgcaat acctcgtact gccggtcatc accctgtcgc tgatcccgat
      961 gggcgtgatc ggacggctgg tcagggccac cgtgctggac atcctgggcc aggatttcgt
     1021 cggcgcgctg tcggccaaag ggctgacccg ctggccggtc atccgccaca tcgccaagaa
     1081 cgccgccccg ccggtgctgg ccctgatggg attgcagttc ggctatctgc tcggtggatc
     1141 gatcctggtg gagacggtgt tcaactggcc tggctccggc aacctgctga acctcgcgat
     1201 cttccgccgc gacatcccgg tcctgcaggc cacgatcctg gtgctggcaa cattcttcgt
     1261 cctgctcaat ctgctggtcg atatcgccca ggcggcgatc gacccgcgca tccgtcgctg
     1321 aacggagatc cgatatgtca gacataacgc tcgacacgcg ccacgccgca caggccgatg
     1381 cgcctgccgc ccctgccccc ggctactgga aatcggtttc ccagcgcctg ctgcgcgaca
     1441 agacgacggt ggtggtcacg ctgatgctgc tgggcatcct gttcatcacg cttggcgcgc
     1501 cgctggtcac cgattacgat ccctaccagg gcaacgtcct ttcgcgcctg aaacccatcg
     1561 gcaccgccgg ccactggctg ggcacggatg aaaccggccg agacctctgg acccggcttt
     1621 gctacggcgg acgattgtcg ctgctcgcgg ccatcctgcc ggtgacgatc tcgttgtttg
     1681 tcggcgggtt ccttgggatc ctggcgggat atgccggcgg catcgccggg acgttgatca
     1741 tgcgcacgat ggacgtgttc tacgccttcc cctcgatcct gctggccatt gcgatctgcg
     1801 gcctgctggg gaacggcctg tcgaattcga tcatcgccct ggccgtggtg ttcattccac
     1861 ccatggtgcg ggtatcggaa acagtaacga cgcaagcccg caacctcgac ttcgtggagg
     1921 cggcgcgggc ctccggcacc accacgatgc agatcatccg ccaccatgtg ttggcaaacg
     1981 ttctggggcc gatcctggtc tatgcgacca gcctgatcag cgtgtcgatc atcctgtcgg
     2041 cgggcctgtc cttcctcggg cttggcgtca ccccgccgca gccggaatgg ggccttatgc
     2101 tgaattcgct gcgccaggcg atctatgtgc agccctacgt ggcagccctg cccggcctga
     2161 tgatcttcct gacctcgatg tgcttcaacc tcatgagcga cggattccga agtgcgatgg
     2221 atgtgaggct caacaaatga gtgctgctct gcgacaagac cccagggacc gcggcggccc
     2281 ggcccagccg ctgctgatcg tggaaaatct gaccaagcac ttcccggtgc gcggcggtct
     2341 gctgaaccgc caggtcgcca cggtgcaggc ggtcgatgac gtatcgttct cggtccgcaa
     2401 gggcgaaacg cttggcatcg tcggagaatc cggctgcggc aaatccacca ccgcgcggtt
     2461 gctgatgcgg ctgatggtcc cggatgccgg atcggtcgtg ttcgacggcg acgcggtcgg
     2521 cgagaagcgc gggatttcgg tcagcgaaat gcgccggcag atgcagatgg tgttccagga
     2581 cagctacacc tcgctgaacc cgcgcatgac catggccgag agcatcgcct atgccccgcg
     2641 catgcacggc ctggcgcgca aggacgctta cgaccgagcg gaaagcctgc tgtcgcgcgt
     2701 cggcctgaac ccgcgtcaat acgcgcgtcg ttacccgcat gagctgtcgg gcggccagcg
     2761 ccagcgcgtc aacatcgccc gggcactggc gctggagcca cgcctggtga tcctggatga
     2821 ggccgtagcg gcgctggaca agtcggtcga ggcgcaggtg ctgaacctgc tgggcgacct
     2881 caagcaaagc ctgaatctga cctacgtgtt catcagccac gacctgaacg tcgtggagta
     2941 catgagcgac cgggtcatgg tgatgtatct cggccgcgtg gtcgaagccg gttcggtgga
     3001 atcgatctac cggcacccaa ggcatcccta cacgcgtgcg ttgttcgcat cgaagccgac
     3061 gcttgacccg gaccggcgca cgacggagat gccgctgacc ggcgatccgc cgaacccgat
     3121 caacccgccc tccggctgcc gcttccgcac ccgttgcgtc tttgcccagc cgcggtgcgc
     3181 ggaaactgtg ccgccattgg cggcgccatt cgatggcgat gccgatccga cccatgtggt
     3241 cgcctgtcac atcccagagg cccaatcggc caaggcggtg catcatgcat aacgatcccg
     3301 tcctgcgcgt gcaggacctg acggtgcgct ttacccgccg caacaccgat gtggcggcgg
     3361 tcaac
//
LOCUS       DASWQN010001263         2532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1574293, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001263 DASWQN010000000
VERSION     DASWQN010001263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574293"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="VGC82_21390"
     CDS             complement(<1..1588)
                     /locus_tag="VGC82_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0425886.1"
                     /translation="MQTYKAPLRDMRFVLHELHDSAALTRLKGLEDVSPELMDSILEE
                     AAKLAEEVLLPTNAPGDELGCVLENGVVRTPKGFKEAYGIFRDGGWTALASDPRYGGQ
                     GLPESLNKLVEEMICSSNLAFSLYPGLTHGAYQAIASHASDELKDTYLPKMVDGTWSG
                     TMCLTEAHCGTDLGMLRTRALPMSDGSYKVSGSKIFISAGEHDLTENIIHLVLARLPD
                     APKGVRGISLFLVPKFIPAADGRPGARNGVVCTALEHKMGIKGSATCQLTFEDATGWL
                     VGQPNKGLATMFTMMNSERLSVGIQGLGAAETSYQSAVAYARDRIQGRSLSGAKFADK
                     AADPIIVHPDVRRMLMTIRSYTEGCRALGGWVARELDEMERSDDPDVRKRAEDFTALM
                     TPVVKALFTDLGFEATSIGMQVYGGHGYIRDHGMEQLVRDGRISMIYEGTNGIQALDL
                     VGRKLPQGTGRMLQTFFHPVSEFIELHSSHAQLGEMIRGFGKAFGALQLATRHIAAKG
                     MADPEEAGAAATEYLRLFGLV"
     gene            complement(1646..>2532)
                     /locus_tag="VGC82_21395"
     CDS             complement(1646..>2532)
                     /locus_tag="VGC82_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0425887.1"
                     /translation="KSNALDDKTIELLGKSIEVVRQKFKAMVIYNEGSNFSLGANLGL
                     ALFAANIAAWGEIEMSIAAGQQAYKALKYAPFPVVAAPSGMALGGGCEIVLHADAVQA
                     HAETYLGLVECGVGLVPGWGGCGEMLARWRAEPKLPRGPMPAPAKVFEGISTADVSKS
                     AHQAMERKFLRPSDGISMNRDRLLADAKQRALSMVDGYAPPKPPEFVLPGTSGRAGLN
                     AAAEGFHRRGLATGHDLVVADALAEVLCGGDTDIIDVVSEQQILDLERRAFMRLARTA
                     PTLARIEHTLETGKPLRN"
BASE COUNT          430 a          853 c          767 g          482 t
ORIGIN      
        1 ccaccagccc gaacagccgc aggtattcgg tcgcggcagc acccgcttcc tccggatcgg
       61 ccatgccctt cgcggcaata tgccttgtcg ccagttgcag cgcgccgaac gccttgccga
      121 accccctgat catctcgccc aactgcgcgt gactggaatg aagttcgata aactcgctga
      181 cggggtggaa gaacgtctgc agcatccgtc cggtgccctg cggcagcttg cggcccacca
      241 gatcgagcgc ctggatgccg ttggtgccct cgtagatcat cgaaatccga ccgtcccgga
      301 cgagttgctc catgccgtgg tcgcggatgt agccgtggcc accatacacc tgcatgccga
      361 tactggtcgc ctcgaacccg agatcggtga acagcgcctt caccaccggc gtcatcaatg
      421 ccgtgaaatc ctcggcacgt ttacggacgt cgggatcgtc ggaccgttcc atctcatcca
      481 gttcccgcgc cacccaaccg cccagcgccc gacaaccctc ggtgtaggag cggatcgtca
      541 tcagcattcg ccgcacgtcg ggatggacga tgatcgggtc tgcggccttg tcggcgaatt
      601 tggccccgct caggctgcgc ccctgtatgc gatcccgagc ataggcgacg gcactttggt
      661 agctggtttc cgcggcaccc aggccctgga tgccgaccga caggcgctcg ctgttcatca
      721 tggtgaacat cgtcgccaat cccttgttcg gctggccgac caaccagccg gtggcgtcct
      781 cgaacgtcaa ctggcaggtc gccgaaccct tgatgcccat cttgtgctcc agcgcggtgc
      841 aaaccacgcc gttgcgggcg ccaggccggc catccgcggc ggggatgaac ttcggcacca
      901 ggaacaggct gatgccgcgc acgcccttgg gcgcatccgg taaccgcgcg agaaccaggt
      961 ggatgatgtt ctcggtcagg tcatgctcgc cggcactgat gaaaatcttc gacccgctga
     1021 ccttgtagct gccgtccgac atgggcagcg cccgggtccg cagcatgccc aggtcggtgc
     1081 cgcaatgcgc ctcggtcagg cacatcgttc ccgaccaggt gccatcgacc atcttcggca
     1141 ggtaggtgtc cttcagctca tcggatgcat ggctggcgat tgcctgataa gcgccgtggg
     1201 tcaggccagg atacaggctg aaagcaagat tggacgagca gatcatctcc tcgaccagct
     1261 tgttcaggct ttcgggcagg ccttggccgc cgtagcgcgg atcagaggcg agcgcggtcc
     1321 agccgccgtc gcgaaaaatg ccgtaggcct ccttgaagcc ttttggcgtg cgcacgacgc
     1381 cattttccaa cacgcagcca agctcgtcgc ccggggcgtt ggtcggcagt agcacttcct
     1441 cagccagctt ggcagcttcc tccaggatgc tgtccatcag ttcgggggac acgtcctcca
     1501 ggcccttgag tcgcgtaagg gctgcgctgt cgtgcagttc gtgcaggacg aagcgcatgt
     1561 cgcggagtgg cgctttatag gtctgcattg gggctacctt gttacgcgtt ggatcaatcc
     1621 tgaagtctgg ctgaaagctg gcatcctaat ttcgcagcgg tttgccagtt tcaagcgtgt
     1681 gctcgatccg ggccagtgtc ggggctgttc gcgcgagccg catgaaggca cgccgctcca
     1741 gatcgaggat ctgctgctcg ctgacgacgt cgatgatgtc ggtatcgccg ccgcacagca
     1801 cttccgccag cgcatcggcg acgaccagat cgtggccggt tgccagcccc cgccgatgga
     1861 acccctcagc cgccgcgttc aacccggctc ggccggacgt tcccggcagc acgaactcgg
     1921 gcggcttcgg tggcgcgtag ccatcgacca tcgacagcgc ccgctgcttg gcatccgcca
     1981 gcaggcggtc ccggttcatg ctgatgccat cgctcgggcg gaggaacttc ctttccatcg
     2041 cctggtgcgc cgatttggag acgtcggccg tgctgatgcc ctcgaacact ttcgccggag
     2101 cgggcatcgg accacgcggc agcttcggtt ccgcccgcca gcgcgccagc atttcaccgc
     2161 acccacccca gccggggacc agcccgacgc cgcactcgac cagcccgaga taggtttcgg
     2221 catgagcctg caccgcgtcg gcatgcagca cgatctcaca cccgccaccc agcgccatgc
     2281 cggagggtgc cgcgacgacg gggaaggggg cgtatttcag cgccttatag gcctgttgcc
     2341 cggctgcgat gctcatctcg atttctcccc aggcggcgat gtttgcggca aacaaagcga
     2401 gtccaaggtt ggcgccgagc gagaaattgc tgccttcgtt atagatcacc atcgccttga
     2461 acttctgccg cactacctcg atcgacttgc ccagcagctc gatggtcttg tcgtcgagcg
     2521 cattggactt cg
//
LOCUS       DASWQN010001264         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_519376, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001264 DASWQN010000000
VERSION     DASWQN010001264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519376"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1644)
                     /locus_tag="VGC82_21400"
     CDS             complement(<1..>1644)
                     /locus_tag="VGC82_21400"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY0425888.1"
                     /translation="CHYRIAVPSAKVGLPEVLIGILPGGGGTQRLPRLVGAQTALDLI
                     TSGRHVPAPEAAMLGIIDEVLPEGANLREAAIAHASKIAAVRPLPRVRDKTVTAEPGV
                     FDAMRKSIARRARNQKAPYSCIAAVEAGCTLPFDDGMQREAELFAELESSDEAKALRY
                     AFFAEREVAKLPDLPTNVAPRDFTAPAVIGAGTMGGGIAMSFADFGYDVKIMDATQEA
                     LDRGMERIRNNYATSVKRGSLAEDEMQRRLARIHPVAGYDDIGDCDVVVEAVFERMDV
                     KKPIFAKLDEVMKPGAFLFSNTSALDMDELAAETKRPEYVAGTHFFSPANVMKLLEVV
                     RPSKASPETLMTAMAMGRRIGKISAMAGNTDGFVANRSRAPFNSEMVILLEEGCLPEQ
                     VDKVMIDFGYPMGPFAVGDLAGLDIGAEGRKRRALANPDYRKLPIADKLVEMGRYGQK
                     TSAGWYRYEKGDRTPHPDPEVAAIIKSVAAEMGVPQKVFTDTEILRRLLFASVNEACR
                     ILEEGKAYRASDIDVMWLHGFGFPRYRGGLMYWADGIGVR"
BASE COUNT          278 a          549 c          512 g          305 t
ORIGIN      
        1 gcggacgccg atcccgtcgg cccagtacat caggccaccg cgatacctcg gaaacccgaa
       61 cccgtgcagc cacataacat cgatatcgct ggcccgatat gccttgcctt cctccaggat
      121 acggcaagcc tcgttgaccg acgcgaacag caatcgccgc aggatctcgg tgtcggtgaa
      181 aaccttctgc ggcacgccca tctcggctgc gaccgacttg atgatcgcgg cgacttccgg
      241 atcgggatgc ggcgtgcgat cacccttctc gtaacgatac catccggcgc tagttttctg
      301 gccgtaacgg cccatctcca ccagcttgtc ggcgatcggc agtttacggt aatcgggatt
      361 ggcgagcgcc cgccgtttgc gcccttccgc gccgatatca agacccgcca ggtcgcccac
      421 cgcgaacggc cccatcggat agccgaaatc gatcatcacc ttgtccacct gctccggcag
      481 gcagccttcc tccagcagga tgaccatctc cgaattgaac ggcgcgcgcg accggttggc
      541 gacgaaccca tcggtgttgc ccgccatcgc cgagatcttg ccgatgcgcc gccccattgc
      601 catggcggtc atcagcgttt ccggcgacgc cttcgacgga cgaacgactt ccagcagctt
      661 catcacgttg gcgggcgaga agaaatgcgt gccggcgacg tattccggcc gcttcgtttc
      721 cgcggcaagt tcatccatgt ccagcgccga ggtgttggag aacaggaacg ctcccggctt
      781 catgacctcg tcgagcttgg cgaaaatcgg cttcttcacg tccatccgct cgaacaccgc
      841 ttcgacgacg acatcgcagt cgccgatgtc gtcgtagccg gcgaccgggt ggatgcgggc
      901 caggcggcgc tgcatttcgt cttccgccag gctgccgcgc ttgacggagg ttgcatagtt
      961 gttgcggatc cgctccatgc cgcggtcgag cgcttcctgg gtcgcgtcca tgatcttcac
     1021 gtcgtaaccg aaatcggcga acgacatggc gatgccgccg cccatggtgc cggccccaat
     1081 gactgccggg gcggtgaagt cacgcggcgc cacattggtt ggcaggtcgg gcagtttcgc
     1141 aacctcgcgt tcggcgaaga aagcgtagcg cagcgccttt gcttcgtccg atgattccag
     1201 ttccgcgaac agttcggcct cgcgctgcat gccgtcgtcg aacggcagcg tgcagccggc
     1261 ttccaccgcg gcaatgcagc tatagggcgc tttctggttg cgtgcccggc gggcgatcga
     1321 cttgcgcatg gcgtcgaaaa cgccgggttc ggcggtgact gtcttgtcgc gcacgcgcgg
     1381 caacggcctg acagcggcga tcttgcttgc atgcgcgatg gcggcctcac gcaggttggc
     1441 accttccggc aggacctcat cgatgatgcc gagcatggcg gcttccgggg cggggacgtg
     1501 gcggccggag gtgatgaggt ccagcgcggt ttgcgctcca accagacgcg gcaggcgctg
     1561 cgttccgcca ccaccgggca ggatgccgat cagcacctcc ggcaagccga ccttcgcgga
     1621 gggcacggca atgcggtaat ggca
//
LOCUS       DASWQN010001265         2724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_782242, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001265 DASWQN010000000
VERSION     DASWQN010001265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782242"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VGC82_21405"
     CDS             <1..738
                     /locus_tag="VGC82_21405"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEY0425889.1"
                     /translation="LLQWLRLFAVPSALSILATYAVLRWTQRVRLQQPVATEIELPAL
                     TLCGKLAALGIAATAVVLLLSSGFDIQLGLPTFLASIATAVLVLCCSKTGPFAVVKSI
                     SWGVLPLVAGLFVLVEALQKTGVTDDLAGLLRELVQWSATGAAWTAGLALAFGCNLVN
                     NLPAGLIAGRVVQLAAVPEHVRVAVLMAIDLGPNLSVTGSLATILWLTALRREGLRVG
                     AWSFLKLGVLVMPPALALAIAGAFVFG"
     gene            1386..>2724
                     /locus_tag="VGC82_21410"
     CDS             1386..>2724
                     /locus_tag="VGC82_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0425890.1"
                     /translation="MQAVNLPTASMVNALRAIVGDRGILTDASDTAPYSEDWRRLYQG
                     RTAAVIRPGTTEELAAVVRLCAGFGTPIVPQGGNTSMVGGAVPNQDGSELVLSTARLN
                     RIRDLDPADMTLTIEAGVTLKAAQTAAAEAGCLLPLSISSEGTAQIGGVLAVNAGGNN
                     TVRYGNARDLVLGLEVVLPDGTIWNGLRRLRKDNTGYCLRQLFVGSEGTLGIITAAVL
                     KLVPQPKEIAVALCGVASPEAALSLFTRFQAFDAASISAFELMSGLGMSFVLKQIPNA
                     TMPLETAAEFYVLVELASPRPGAGLRGMLEQILEQALEDDIIGDAAIAESDTQRAAIW
                     RLREEHSEAQKREGASVKNDVSVPVSKVPAFIRKATAACETLMPGVRVVPFGHMGDGN
                     IHFNLEQPLGADAAWVLAQDHAIMDAVNEVVREYDGSFSAEHGIGKLKPYMMPD"
BASE COUNT          425 a          857 c          922 g          520 t
ORIGIN      
        1 ctgttgcaat ggctgcgact tttcgcagtg ccgtccgcac tgtcgatcct cgcgacttat
       61 gccgtgctgc gctggaccca gcgggttcgg ctgcaacaac ccgttgcgac cgaaatcgag
      121 ctccctgcac tgacgctttg cggcaagctc gcggcacttg gcatcgccgc caccgccgtt
      181 gttctgctgt tatcctccgg ctttgacatc caactcggtt tgccgacgtt cctggccagc
      241 atcgcgacgg cggtgctggt gctgtgctgt tccaaaaccg ggccgttcgc cgtggttaag
      301 agcatctcct ggggcgtact gccgctggtt gccggactat tcgtgctggt ggaggcgttg
      361 caaaagaccg gcgtcaccga cgatctggcc ggattgctgc gcgaactggt tcaatggtcg
      421 gccactggcg cggcctggac tgccggcctg gcgctcgcgt ttggctgcaa cctggtgaac
      481 aatctgccgg ccgggttgat cgcggggcgg gtggttcagc tcgccgctgt ccccgagcat
      541 gtgcgggtgg cggtcctgat ggcaatcgat ttaggtccga acctgtcggt gaccggatcg
      601 ctggccacca tcctttggct gaccgctttg cgccgcgagg gtctgcgggt cggcgcctgg
      661 agctttctga agcttggcgt gctcgtgatg ccccccgcgc tggcactggc gatcgccggt
      721 gccttcgtat ttggctgaac agactccgaa tgcgatcggc atggccaaga cggcggcttg
      781 ttccatcctg tccggtcgtg ctctgtccgg tcgtgctctt ggcgcggccc gcggaaggcg
      841 accagataca gtgcgtcttc gaggcaatga taccgaagta gtaccggaca ccgccagagc
      901 gtatggcacc cgatccgatc cggtgttccg ctggtttcgg cgctccagcc gccggtcgcc
      961 gatacccaag ggagcaagtc gatgcctgtc acccgccgca cattcctcgg ctccggcgcc
     1021 atccccgccg ccggctcgtc ccgggcggct gcgccgctcg atccgtcgcc ggccggtggc
     1081 atcactgccg tcgcaggaga cccgcggcgg tgcagcttta tccggactag tgcctgtcgg
     1141 aggagggtca gacagtgctg atccgcgacc tcggtcacct tacggcattg cgacgtgcgc
     1201 cgctgaatcc ccaggtctcg atcccgaggt gcagcgacta tggttcgccg atcgaggcga
     1261 atcggaccgg ttgcgccgac cgtcgctgga ggactgggga cgagcctacg gggtgcggca
     1321 gtagccgttc ggcagccggc gcggtaaagg atgcgctgga ggatccgtcg atgcccgata
     1381 tcgcgatgca agctgtcaat ctgcccaccg ccagcatggt gaacgccctt cgcgcgatcg
     1441 ttggcgaccg gggtatcctg accgacgctt cggacaccgc gccatacagc gaagactggc
     1501 gccgtctcta ccaaggccgc accgctgcgg tgatccggcc cggcacgacc gaagaactcg
     1561 ccgccgtggt gcggttgtgc gccgggttcg gcaccccgat cgtgccgcaa ggcggcaaca
     1621 cctccatggt tggcggcgcg gtgccgaacc aggacgggtc ggagctggtc ctgtccaccg
     1681 cccgcctgaa ccgcatccgc gatctcgatc ccgccgacat gacgctgacg atcgaggctg
     1741 gagtgacgtt gaaggcggct cagacggcgg cggcagaggc tggatgcctg ctgccgttgt
     1801 cgatttcctc ggaaggcacc gcacagatcg gtggcgttct ggcggtgaac gccggaggca
     1861 acaacaccgt gcgctacggc aatgcccggg atctggtgct gggcctggag gtggtgttgc
     1921 ccgacggcac catctggaac gggctgcgcc ggctgcgcaa ggacaacacc gggtattgcc
     1981 tgcgccagct ctttgtcggg tccgaaggca cgctgggcat catcaccgcc gccgtgctga
     2041 agctggtgcc tcagccgaag gaaatcgccg tggcactttg cggtgtcgca tcgccggagg
     2101 cggcgctatc gctgttcacg cgattccagg cgttcgacgc ggcctccatc agcgcgttcg
     2161 aactgatgtc cggcctgggg atgtcgttcg ttctgaagca aatacccaac gccacgatgc
     2221 cgctggagac ggcggcggaa ttctatgtgc tggtcgaact tgcctcgccc cggcccggtg
     2281 ccggcctgcg cggcatgctg gagcagattt tggaacaggc tctggaggac gacatcatcg
     2341 gcgatgcggc catcgcggaa tcggacacgc aacgggctgc gatctggcgg ctgcgggagg
     2401 aacactcgga agcgcagaag cgcgaagggg cgagtgtcaa gaacgacgtt tcggtgccgg
     2461 tttccaaggt tccggcgttc atccgcaagg ctaccgctgc ctgcgaaacc ttgatgccgg
     2521 gcgtccgtgt ggttccgttc gggcacatgg gcgacggcaa catccatttc aatctggaac
     2581 agcctctcgg cgccgacgcg gcttgggtcc tggcgcagga ccacgcgatc atggacgcgg
     2641 taaatgaggt tgtgcgggag tatgacggga gtttctcggc ggagcatggg attgggaagt
     2701 tgaagcctta catgatgccg gact
//
LOCUS       DASWQN010001266         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2366899, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001266 DASWQN010000000
VERSION     DASWQN010001266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2366899"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VGC82_21415"
     CDS             complement(<1..646)
                     /locus_tag="VGC82_21415"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425891.1"
                     /translation="MPLASELAPKAAKLGLRGTVAIEEGYAFFHSFPDHRVDEADIEA
                     KAPVLAEFIEASCARHNFARRPVAVGFSNGAIMAAALLLAHPDLLAGAILFRPLSPFT
                     YDLPHRLDSTPVLIIDGAKDSRRSPGDGVRLAERLVSAGALVTHHVLPARHSITAEDK
                     RIASEWLQAIRIWYTYSLRRGNVINGKSLGGSAAVEQIAFRRHRYVEGVHHTTQR"
     gene            complement(1018..1431)
                     /locus_tag="VGC82_21420"
     CDS             complement(1018..1431)
                     /locus_tag="VGC82_21420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEY0425892.1"
                     /translation="MRVVFDTNVVVSALVFGGRLSWLRAAWAARTVTPVVCRETVAEL
                     LRVLAYPKFRLTREDTDILLAQFLPFCETASLPSAWPPMPAACRDRDDVVFIALTLTS
                     GAEGLVSGDADLTVLGDTVRVLSVAGLWRRLEREG"
     gene            complement(1438..1698)
                     /locus_tag="VGC82_21425"
     CDS             complement(1438..1698)
                     /locus_tag="VGC82_21425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY0425893.1"
                     /translation="MQWPPYFLTLPIMLAKITSKNQLTLPKRVVQALGFPTHLDVEVK
                     DGRLILTPPSVGAADAVRSKLEALGITESDVADAVAAARRWG"
     gene            complement(1741..>2162)
                     /locus_tag="VGC82_21430"
     CDS             complement(1741..>2162)
                     /locus_tag="VGC82_21430"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0425894.1"
                     /translation="MAYTKVKYIIEQTVASGLIYLNSHARDFQSPEIRPYLDKYLRGS
                     NGYAAEDRVKLMKLLWDSIASEFGGRHELYEINYAGSTEEIRRYCLFGAQASGNAAAF
                     KGFAEQCMAEYDLNGWTVPDLTDPGDLSYHLMRGSRS"
BASE COUNT          433 a          662 c          656 g          411 t
ORIGIN      
        1 cgcgctgtgt ggtgtggtga acgccttcga cgtacctgtg ccgccggaag gcgatctgtt
       61 cgacagcggc cgaccctcct aatgatttac cgttgataac gttgccgcgc cgcaggctat
      121 acgtatacca aatccgtatc gcctgaagcc actcacttgc aattcgcttg tcctcggcgg
      181 taatcgagtg gcgggccggc agcacatgat gggtcaccaa tgctccagca gaaaccagcc
      241 gctcagccag tcgcacgcca tcgccgggcg atctgcgact gtccttcgca ccgtcgataa
      301 tcagcaccgg cgtactgtcc aggcggtgcg ggagatcgta agtgaacgga gagagcgggc
      361 ggaacaggat tgcccccgcc agtaggtcag gatgggccag caacagggct gccgccatga
      421 tcgcaccgtt ggagaagccg acagctactg gccgcctggc gaagttgtgg cgggcgcagg
      481 acgcttcgat gaattccgcc agcacaggcg ctttggcctc aatgtcagcc tcatccaccc
      541 gatgatcggg aaagctatgg aagaaggcat atccctcctc tatggcaacc gtgccgcgaa
      601 ggccgagctt tgccgcttta ggtgccaatt cggaggccaa aggcacaagg tcgaattcgt
      661 tgccgccaga gccatgtagc agaaccagcg gcgcaccgac ggtatctgtg ccaggaaggc
      721 agcaaaaggc atttatcggg tcaggcatag gagcaaggcc gttcaattgg tgagagatga
      781 attgatcttg tagcttacag gtgaagtctg cttacagatg agggccgcat agcctcccct
      841 cccttccgct ccagctgcct cagcgtgcag tttggacaga tcgtgtagtg agccattgga
      901 cgcctggtaa ctgcgggcat ccgctttcgg gacctttcca tggctgattc gcgtccgacc
      961 cgggtcggta gcggtcgttc aaacccattt atggcagtga gaaagattca atttcgacta
     1021 cccctcgcgc tccaacctcc gccagaggcc tgcgaccgag agcacccgaa ctgtatctcc
     1081 cagcaccgtc aggtcggcgt cgccactcac gaggccttcc gcaccagacg tcagcgttag
     1141 cgcgatgaac accacgtcgt ctcggtcgcg gcacgcagcg ggcatcggtg gccacgcaga
     1201 cggcagggat gcggtttcgc aaaatggtag aaattgagcc agcaggatgt ccgtatcttc
     1261 tcgagtcagc cggaattttg ggtaggccag gacgcgcaga agttcggcca cggtttcccg
     1321 gcacaccacg ggcgtcaccg tccgggcggc ccaggcggca cgcaaccagc tcagccggcc
     1381 accaaatacc agcgccgaga ccacgacgtt ggtatcgaag acaacccgca gcttgtgtca
     1441 gccccaacgc cgggcggcgg cgaccgcgtc ggcgacatcg gactcagtga tgccgagtgc
     1501 ctccagcttg ctgcgaacgg cgtcggccgc accgacactg ggcggtgtca ggatcaggcg
     1561 gccgtccttt acctcaacgt ccagatgggt cgggaagcca agcgcctgaa cgacccgctt
     1621 gggaagcgtg agttggtttt tggaggttat tttcgcgagc atgatcggca atgtaaggaa
     1681 gtaaggcggc cactgcaact gccttcgtca cgtttggcgc cctcgaccaa gcggcagcca
     1741 tcaagatctg ctgccgcgca tcaggtggta gctcaagtcg ccagggtcgg tcaggtccgg
     1801 cacggtccag ccattcaggt cgtattccgc catgcattgt tcggcgaagc ccttgaacgc
     1861 cgccgcattg cccgatgcct gcgcgccgaa caaacagtaa cggcggattt cctctgtcga
     1921 gccggcgtag ttgatctcgt acagctcgtg ccgaccgccg aactcggacg caatgctgtc
     1981 ccacagcagc ttcatcagct tcacccgatc ctcggcggca tagccgttgg acccgcgcag
     2041 atacttgtcg agatacggcc ggatctcggg gctctggaag tcccgggcgt ggctgttcag
     2101 gtagatcagg ccggacgcga ccgtctgttc gatgatgtat ttgaccttcg tataggccat
     2161 cg
//
LOCUS       DASWQN010001267         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_916426, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001267 DASWQN010000000
VERSION     DASWQN010001267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916426"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VGC82_21435"
     CDS             <1..1094
                     /locus_tag="VGC82_21435"
                     /inference="COORDINATES: protein motif:HMM:NF019944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HEY0425895.1"
                     /translation="AAGVPGLRPQDVTVVDSNLHLLVQAGDGNDPRAKSAMEEDVRQK
                     TEARLVQGVELMLERSLGSGHVHAEASIRMNFDKVSETQEKFDPDGSVVRSTQNVTSN
                     NKTTDKIGPVSLQNNLPNADAGATASGSQEGRQEETTNYEIGKTVRAIVHDQPQIERI
                     TLAVMVDGVDEVGADGKRNWKAREPADLERIEKLVKSAVGFDDKRGDRLDVVSMPFVA
                     TQEAVEAAPSTRPGKTNGDLQTFVQMIAFGVVGFGIIILTARSIFTALGKQPASLSIA
                     GGGVPGEIGGTSATGIGQSGGGGGALGIGHSRAEAGAAAADDDETMTMSNIQGQLRAS
                     SIRKVTQLVDRHPDTTLGIIRGWMAEDAG"
     gene            complement(1199..>1601)
                     /gene="purH"
                     /locus_tag="VGC82_21440"
     CDS             complement(1199..>1601)
                     /gene="purH"
                     /locus_tag="VGC82_21440"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812402.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY0425896.1"
                     /translation="VTKRAPTGPEMADLLFAFRVCKHVKSNAIVYAKSGATVGIGGGQ
                     PNRVDSARIAAWKSEEAAKAAGLSERMTHGSVVASDAFFPFADGLEVAVAAGATAVIQ
                     PGGSIRDAEVIAAADAAGLAMVFTGMRHFRH"
BASE COUNT          317 a          482 c          544 g          258 t
ORIGIN      
        1 ttgcggccgg cgtgcccgga ctccggccgc aggatgtgac ggtggtggat tcgaacctgc
       61 atctgctggt ccaggctggg gacggcaatg acccacgggc gaagtcggcg atggaggaag
      121 atgttcgcca gaagaccgaa gcgcggctgg tgcagggggt cgagctgatg ttggagcgca
      181 gccttgggtc cggccacgtg catgccgagg cctcgatccg gatgaacttc gacaaggtca
      241 gcgagacgca ggagaaattc gacccagacg ggtcggtggt ccgcagcacg cagaacgtga
      301 cctccaacaa caagaccacg gacaagatcg ggccggtctc cttgcagaac aatctgccga
      361 atgccgatgc cggtgcgacc gcgagcggca gccaggaagg gcggcaggag gagacgacga
      421 attacgagat cggtaagacc gtccgggcga ttgttcacga tcagccgcag atcgagcgga
      481 ttacgctggc ggtgatggtc gatggcgttg acgaggttgg cgccgatggc aagcgcaact
      541 ggaaggcgcg cgagccggcc gatctggagc ggatcgagaa actggtgaaa agtgccgtcg
      601 gcttcgatga caagcggggc gaccggctgg acgtcgtcag catgccgttc gtcgccacgc
      661 aggaggctgt ggaggcggca ccttcgacac ggcctggcaa aacgaatgga gaccttcaaa
      721 ccttcgtcca gatgatcgcg ttcggagtgg tcggtttcgg gatcatcatt ctgacggcac
      781 gttcgatttt cactgcactg ggcaagcaac cggcgagcct ttcgatcgct ggtggcgggg
      841 tgcccggaga aattggcggc acttcggcca ccggcatcgg gcaatcgggc ggtggaggcg
      901 gcgcgctggg gatcggacac agccgggccg aggctggagc agcagcggcc gacgacgacg
      961 aaacaatgac gatgagcaac attcaggggc agttgcgggc gtcgtcgatc cgcaaagtca
     1021 cgcagttggt ggaccggcat ccggacacca cgctgggcat tatccgggga tggatggcag
     1081 aggatgcggg ttaaccactt aggccttgtt ccgcggcagg acgtagctcc aacggccgga
     1141 cgacgtgcct gagccaatcc acctataagt acccgcgttg ggaccgaccc tggcggcttc
     1201 agtgccggaa atgacgcatc ccggtgaaca ccatcgctaa cccagctgca tccgctgccg
     1261 ctatcacctc ggcatcccgg atcgacccgc ctggctggat gacggccgtc gcgcccgccg
     1321 cgaccgccac ctccaaacca tcggcgaacg ggaagaatgc gtccgaggcc accacgctcc
     1381 cgtgggtcat ccgttccgac aagccggccg cctttgctgc ttcctcgctc ttccaggcgg
     1441 cgatccgcgc cgagtccacg cggttcggtt gcccgccacc gattccgacc gtggcgccag
     1501 acttggcata aacgatcgca ttcgacttca catgcttgca tacccggaag gcgaacaata
     1561 agtcggccat ctccggcccg gtcggcgcgc gtttggtgac g
//
LOCUS       DASWQN010001268         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_652127, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001268 DASWQN010000000
VERSION     DASWQN010001268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652127"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(426..875)
                     /locus_tag="VGC82_21445"
     CDS             complement(426..875)
                     /locus_tag="VGC82_21445"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY0425897.1"
                     /translation="MSKIEDNKTIVGRWFKDFWGPTCNLAIVDELAAPDMLLQYSLHA
                     PRRGHDDIKAFMAGFREAFPDLSFSGAADLIAEGDYVVGRWEGGGTHSGPAFSDFLIG
                     SLPAATGRKMRFSGTTVLRLQDGKIAEEIGLDDGVTALQQLGLMRAA"
     gene            complement(1029..1436)
                     /locus_tag="VGC82_21450"
     CDS             complement(1029..1436)
                     /locus_tag="VGC82_21450"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY0425898.1"
                     /translation="MCKEDDNKALVGRWFKDFWSAPSDLAVVDELAAPDVLLQYSPDT
                     PRRGRDAVRAFRTSLRDAFPDLVIAGASLIADRDMVVVRWSGIGTHTGPAFDDFKIGP
                     LPAASGRTVRLSGTTAVRVENGRIAEEAIWIAA"
     gene            complement(1540..2202)
                     /gene="alkB"
                     /locus_tag="VGC82_21455"
     CDS             complement(1540..2202)
                     /gene="alkB"
                     /locus_tag="VGC82_21455"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HEY0425899.1"
                     /translation="MPPETDAPSLGLLEPGPRTMALGPGAVLLGGFALAAEADLLAGL
                     DGVLGSSPFRHMVTPSGFPMSVAMSNCGVAGWVSDRTGYRYDRSDPETGRSWPDMPYA
                     FGRLAADAAERAGFPGFAPDACLINRYVPGARLTLHQDKNERDFGQPIVSVSLGLPAI
                     FLWGGQTRAERPRRVPLVHGDVVVWGGPARKTFHGVLELAPGEHRLTGPVRYNLTFRK
                     AL"
BASE COUNT          414 a          739 c          716 g          382 t
ORIGIN      
        1 aatgtcggtg cagcaactgc gacggttgat tccgacccgt cagtccaacg aaaaaacccc
       61 cgaaggcttg aacctcgggg tttttgattt tgatcggccg tatgaaggcg atgccctcga
      121 ctgcaggggg agccttccgg ctgaaaccac cggccgatgg tgttgaagac tgccgttgcc
      181 aagtttaggg aatataaatc cggaccctgc caccttcgca gactgccgca attgcatgtc
      241 aattggcacg aaggtgacga cacggacaaa gggctgggtc accgctcccg acttaccata
      301 aactggcgat tatgcggtta gtccctgtag cagagccatg tagacaacgc taagctggaa
      361 caccctgcga acacgctggg ctgatgccgc cgccgctcgg caccggcggc ggccgcatct
      421 tctgctcagg cagcgcgcat gagaccaagc tgctggagcg cggtcacgcc atcgtccaga
      481 ccgatctcct ctgcgatctt gccatcttgc aggcgtagca cggtcgtgcc gctgaagcgc
      541 atcttccggc cggttgccgc tggcagggat ccgatgagaa aatcactgaa ggctgggccg
      601 gaatgcgttc ccccaccttc ccagcgaccg accacgtagt cgccctcggc gatcaggtcg
      661 gcagcgcccg agaagctgag gtcaggaaag gcttcacgaa atccggccat gaacgccttg
      721 atgtcgtcgt ggccgcgacg tggcgcatgc agcgagtact gcaagagcat gtcgggggcg
      781 gccaactcgt cgacgatggc gagattgcag gttgggcccc agaagtcctt gaaccagcgg
      841 ccgacgatgg tcttgttgtc ttcgatcttc gacatgttgg cgtctcctgt tgaccatttc
      901 tgaaactcag ccggtggatg gtggcacccc gcccgacctg accattcctg gagccggcaa
      961 gccgccggcg aactccggtt cctgttctcg aatcgaacat gggcggatct ggattcattc
     1021 tgacggcatc aagcggcgat ccagattgct tcctccgcga tcctgccatt ctcgacccga
     1081 accgcggtgg ttccactcaa ccggacggtg cggcccgacg ctgccgggag gggcccgatc
     1141 ttgaagtcgt cgaaggccgg gccggtgtgc gtgccgatcc cgctccaacg cacgaccacc
     1201 atgtcgcgat cggcaattag gctcgcaccc gcgatgacga ggtcggggaa cgcgtcgcgg
     1261 aggctggtcc ggaacgcgcg caccgcgtca cggccgcgcc gtggcgtatc gggcgagtac
     1321 tgaagaagca cgtccggtgc ggccagctca tcgacaaccg cgagatcgga tggcgcactc
     1381 cagaaatcct tgaaccagcg gccgacgaga gccttgttgt cgtcttcctt gcacatgatc
     1441 cgttgctcct gcttgcgggc cgcttccgct cggcctggat catgcgtagg ccgggccggg
     1501 gcagggcaaa ctccggttct ggtttccaaa accgaaaact cagagcgcct tgcggaaggt
     1561 gagattgtag cggaccggac ccgtcagccg atgctccccg ggtgccagtt caagcactcc
     1621 gtggaaggtc ttgcgggccg gaccgcccca cacgaccacg tccccatgca ccagcggcac
     1681 acgccgtggc cgctcggccc gggtctggcc accccacagg aagatggcgg gcaggcccag
     1741 cgacacggac acgatgggct ggccgaagtc cctctcgttc ttgtcctgat gcagcgtcag
     1801 ccgggcgccg ggcacgtagc ggttgatgag gcaggcatcc ggtgcgaagc cgggaaagcc
     1861 ggcccgttcg gcggcgtcgg ccgcgagcct accgaaggcg tacggcatgt ccggccagga
     1921 ccgcccggtt tccggatcgc tccggtcgta gcggtatccg gtgcggtccg acacccagcc
     1981 cgcgacgccg cagttgctca tcgccaccga catcgggaag ccgctcggcg tcaccatgtg
     2041 acggaagggc gaagagccaa gcaccccatc aagccccgcc agcaggtccg cctccgctgc
     2101 gagggcgaag ccgcccagca gcaccgcgcc aggccccagc gccatcgtgc gcggccctgg
     2161 ttccagtagc cccagcgatg gagcgtccgt ttcaggcggc atcgtggaag atcaccccga
     2221 gcgtatggcg atggccggag cgcacctggc t
//
LOCUS       DASWQN010001269         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2236076, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001269 DASWQN010000000
VERSION     DASWQN010001269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2236076"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VGC82_21460"
     CDS             complement(<1..711)
                     /locus_tag="VGC82_21460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HEY0425900.1"
                     /translation="MALNLFAVPPRVPFLDAVADGWLARRRLSGNDDPLGASRGLILL
                     PTRRSARALAEVFLRVNDGRPMLLPRITAIGALDEAPLALAGALDLPPAVEPMQRLAA
                     LSRLIQAMDGKAGAPRSADRAWLLARELASLMDEAERAGIDLARRLPDAADPEYAAHW
                     TQTLRFLHIVTTAWPAWLADNGVMNPAARQVALLEAQARIWEETPSEDPVLVAGITAC
                     IPAVARLLRVVVGMPGGQV"
     gene            complement(702..1445)
                     /locus_tag="VGC82_21465"
     CDS             complement(702..1445)
                     /locus_tag="VGC82_21465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0425901.1"
                     /translation="MTVSPRSAMVLAAGLATRMRPLTESTPKPLLMLGGCSLLDHALD
                     HLVDAGVETVAVNAHWQADAIAVHLAHRPAPPRTVLLREEKLLETGGGVRAALAVLGA
                     DPFFVVNGDAFWLNGPISALLRLAAVFDDSVDGVLLVQRTSHVHADVGLGDFAVDKWG
                     VPRRRREREVVPYIYAGVQLIHPRLLHEMPPGAFSMNRAWDIALAAGRLRAVVHDGLW
                     FHLSTPPDLAEAEQILQARITGDENWPWR"
     gene            complement(1592..2599)
                     /locus_tag="VGC82_21470"
     CDS             complement(1592..2599)
                     /locus_tag="VGC82_21470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0425902.1"
                     /translation="MNRDDLIAVFLARNGYAPSDAEPLAGDASTRRYLRIRGERRAVL
                     MDAAPPEDVRPFLRIAAHLHGIGLKVPAILAADEIHGLVLEQDLGDDLLSLLLDRGDA
                     PDPLLDAAVDALVIMHRAAPLDGLPLWDAEAMASTALGTLFDWWWPVQFGSPAAEAAR
                     DDVAAGLAAILRPLVAGPRCLVHRDYFAGNLLRLSDQRVGIIDFQDAALGHPAYDLVS
                     LTQDARRDIAPASRDRAVKRYLVARPEIDPTEFAAALTACAAQRHLRVACQWVRLALR
                     DGRPQYLGYGPRTWRLLEDALSQPAAKPLAAALNRWIPAVRRGNPPGLNHPQHDDAGQ
                     Q"
     gene            complement(2596..3084)
                     /gene="tsaE"
                     /locus_tag="VGC82_21475"
     CDS             complement(2596..3084)
                     /gene="tsaE"
                     /locus_tag="VGC82_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY0425903.1"
                     /translation="MPVSSIVSLPDLAATEALAKRLASVARPGDCILLEGPLGAGKTA
                     FARAFLRAASGDPAMEVPSPSFTLVQTYETDIGLVFHYDLWRLEGSASLAELDWEDAL
                     DGITLVEWPDRLGPLRPSDALTITLRLREGEAREATLVGWNGRLGSNHAANAPEAARK
                     PA"
BASE COUNT          583 a         1071 c         1050 g          484 t
ORIGIN      
        1 gacctgtccg ccgggcatcc caaccaccac gcgcaacagg cgcgcaacgg caggaatgca
       61 ggcagtgatg cctgcgacca gcaccggatc ctcggacggt gtttcctccc agatccgcgc
      121 ctgtgcctcc agcagcgcga cttgcctcgc ggcggggttc ataacgccgt tgtcggcaag
      181 ccaggccggc caagccgtcg tgacgatgtg cagaaaccgc agagtctggg tccagtgcgc
      241 cgcgtattcc ggatcggcgg cgtccggcag gcgcctcgcc aggtcgatcc cggcacgttc
      301 cgcttcgtcc atcagcgatg ccagttcccg agccagcagc caggctcggt cggctgatcg
      361 aggtgcgccg gccttgccgt ccatcgcctg gatcagcctg gacagggctg ccagccgctg
      421 cattggttcc accgccggcg gcaggtccaa cgcgccggcc aacgcaagcg gtgcctcatc
      481 cagcgcgccg atcgccgtga tccggggcag cagcatgggg cggccgtcat tgacccgcaa
      541 aaacacctcg gccaaggcgc gagcggatcg gcgggtgggc agcaggatta gcccccggct
      601 cgcccccagc ggatcgtcgt tcccgctcag gcggcgacgg gccagccatc cgtccgccac
      661 cgcatcgaga aagggaaccc gcggcgggac agcgaaaaga tttagcgcca cggccaattt
      721 tcgtcgccgg tgatgcgcgc ctgaaggatt tgttcggctt cagccagatc gggtggcgtc
      781 gagaggtgga accacaaacc gtcatgcacg acagcgcgaa ggcggccggc tgccaaggcg
      841 atgtcccagg cgcggttcat gctgaacgcg cccggcggca tttcgtgcag caggcggggg
      901 tggatcaact gcacgccggc gtagatgtag gggacgacct cgcgctccct ccgccgccgg
      961 ggcacccccc atttgtccac cgcgaagtcg ccaaggccga catcggcgtg gacatgagac
     1021 gttcgctgca ccagcagaac gccgtccacc gagtcgtcga agaccgcggc caaccggagc
     1081 agtgccgaaa tcggcccgtt cagccagaac gcatcgccgt taacgacgaa gaacgggtcg
     1141 gcgccaagga cggccagcgc ggcccggact ccgccgccgg tttccagcag tttttcttcg
     1201 cgcagcagca cggttcgcgg cggcgctgga cggtgtgcca gatggacggc gatggcgtcc
     1261 gcctgccaat gggcgttgac cgccacggtc tcgacaccgg catctacaag gtgatccagg
     1321 gcgtgatcca gcagcgagca tccccccagc ataagcaacg gcttgggggt gctctcggtc
     1381 aacggccgca tgcgggtcgc gagaccggcc gccagaacca ttgcggaacg cggcgacacg
     1441 gtcatggttc agctccgggc atcgctagtg agcatgatcg cgctaggcgg aacgtccaaa
     1501 gcgcgtgaat catgcgatcg gacaaaaacc atgatcgcgc taggcggaag atccaaagcg
     1561 cgtgaatcat gcggtcagac atatccatag atcattgttg gcccgcatca tcatgttgcg
     1621 gatggttcag tccgggtggg ttgccgcgcc gaacggctgg tatccaacgg ttcaacgcgg
     1681 ctgcaagtgg tttggcggcg ggttgcgaca gtgcgtcttc cagaagccgc cacgtccgcg
     1741 gtccgtaacc aaggtattgc ggccggccat cgcgcaaagc aagcctgacc cactggcaag
     1801 cgacccgaag atggcgttgt gccgcgcagg cggtgagggc cgccgcgaac tcggtcgggt
     1861 cgatctccgg ccgggcaacg agataccgct ttaccgcccg gtcgcgggac gccggcgcga
     1921 tgtcccgccg tgcgtcctgc gtcagcgaga caaggtcgta ggctgggtgg ccgagggcag
     1981 cgtcctggaa gtcgatgatc ccgacgcgct ggtccgacag ccgaagcagg ttgccggcaa
     2041 agtagtcgcg atggaccagg catcgcgggc ccgccacgag aggtctcagt atcgccgcca
     2101 gacctgccgc gacatcgtcc cgcgctgctt ccgcggccgg cgagccgaat tggaccggcc
     2161 accaccagtc gaacaacgtg cccaatgcgg tcgaggccat cgcttccgcg tcccatagcg
     2221 gcaagccatc cagcggggcc gctcgatgca tgatcaccag cgcatcgacc gcggcatcca
     2281 gcagcgggtc cggggcgtcg ccccgatcca gcagcaacga cagcaggtcg tcgcccagat
     2341 cctgttcgag gaccaggcca tggatctcgt cagcggccag gatcgctggc accttcaggc
     2401 cgataccgtg cagatgcgcg gcgatgcgca ggaaaggacg aacgtcctcg ggcggcgcgg
     2461 cgtccatcag cacagcccgc cgttcgccgc gaatacgcaa ataccgcctt gtgctggcat
     2521 ccccggcgag cggttcggca tcgctggggg cgtagccgtt gcgggccagg aagacggcga
     2581 tgaggtcgtc gcggttcatg ctggttttcg tgccgcctcg ggcgcgttcg cggcatgatt
     2641 ggaaccgagg cgcccgttcc agcccacaag cgttgcctcg cgcgcttcgc cttcccgcag
     2701 acgcagggtg atcgtcaggg cgtccgacgg ccgtaaaggg cccaaccggt ccggccattc
     2761 caccagcgta attccatcga gcgcgtcctc ccagtctaac tcggccagtg aagcggaccc
     2821 ttcgagccgc cataagtcat aatgaaacac cagaccaatg tcggtttcgt aggtttgcac
     2881 cagggtgaac gacggactgg gcacctccat cgctggatcg cccgacgccg cccgcaggaa
     2941 cgcccgggca aaggcggttt ttccagcacc caagggacct tccagcagga tgcagtctcc
     3001 gggccgggcg accgaagcca gccgcttcgc cagcgcttcc gtcgcggcga ggtcaggaag
     3061 ggagacgatc gaggacaccg gcatcgcgtt aatctggacc aacccccgag tcgcaatcaa
     3121 caagtcacgt aggaagcatg acggttcggg tttgtcggcc ccgcgcctca tgctacaggg
     3181 gccccttg
//
LOCUS       DASWQN010001270         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_652248, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001270 DASWQN010000000
VERSION     DASWQN010001270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652248"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..941
                     /locus_tag="VGC82_21480"
                     /pseudo
     CDS             123..941
                     /locus_tag="VGC82_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019084628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
     gene            942..1250
                     /locus_tag="VGC82_21485"
     CDS             942..1250
                     /locus_tag="VGC82_21485"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY0425904.1"
                     /translation="MATGLLLTAAPSARADDPPVLVFHNHRMEPSRIEVPAHQKFRLL
                     VKNADDAAEEFESTDLNREKLVSPGQTITVFLGPLEPGEYKIFGDFHQDTARGVMVAK
                     "
     gene            1247..>1929
                     /locus_tag="VGC82_21490"
     CDS             1247..>1929
                     /locus_tag="VGC82_21490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425905.1"
                     /translation="MTCRFILFAAALALLAPEQARADFHVRSPYEIDLGELEVEHNGA
                     ASFDRLPENRGAQSYTIEFGTGLTSWWHSEIELGFERSPGFNQSTLVTAAVSENTFQL
                     TEPGQYFADFGFYAEYGQSLTTGRHAGPNEFTFGPLIGKDIGQTSHLLNLFLTRQLGP
                     NQDNHGQTFSYAWQSRWNLWQPLSPAIEIYGDAGTLGRSPKLSQQSLRVGPVGVGSMS
                     LRDLGLGNRG"
BASE COUNT          315 a          616 c          600 g          398 t
ORIGIN      
        1 gccgttggtt gccgatccgc tacgagtctc acgatagtga taatcctcct attgcgtcat
       61 tcgccgtgct tgtgtagctt tgttgcaagt cattcgcatt tgcagctcac gggaaacgtc
      121 gcatgttggt cagcctgatc atcgtctttc gcgaagccat ggaggccggg ttgatcgtcg
      181 gcatcatcct cgccgccacc cagggcgtcg tcggccgcgg ccgctggatc gccggcggga
      241 tcgccggcgg tgtcgccggc gcaagcttgg ttgcggtctt tgcggccgcc ctgtcggacg
      301 cattcgaagg ggccgggcaa gaaatcttca ccgccgcgat cctgggcttt gccgtcgtca
      361 tgctgggctg gcacatcgtc tggatgtcgc gtcacgcccg cggcatggcc gccgagttcc
      421 gcgaggtcgg ccaggccgtt cggctcggcc agcgttcgct ggcggctctg gccggagtcg
      481 tcgcgatcgc ggtgctgcgc gaaggcgccg aagtcgtgct gttcctttac ggcagcatcg
      541 tgggatcgca gacgaccgcg gcatccatcg ccgtgggcgg tatcggcggg ctggctttgg
      601 ctgccgcctt gtcattgctg ctgtatcgcg gcttggtcgc aattccgctg caccacctgt
      661 tcagggttac caattggctg atagcgctgc tggcggccgg catggccggt caggcggcgg
      721 ccatcctgca cagcgccgac ctgctgccgg gtttcgggga acatctttgg gattctagcg
      781 cgctgctgtc cgattccagc atcgccggca aagcgcttca tgcacttgtg ggctattccg
      841 cgcgcccgtc cggtattcag ctcgcggcat gggtgggcac attgcttctg ctggcggtgt
      901 tttcccgcac ggtcagccgg ccgcgcccga tcgccgcggc tatcgcaacc ggcctgttgc
      961 tgaccgctgc gccatccgcc cgcgcggacg acccgcccgt gctggtgttt cataaccacc
     1021 ggatggagcc gtcgcgcatc gaggttcccg ctcaccagaa attccgcctg ctggtcaaga
     1081 acgccgacga tgccgccgaa gaattcgaaa gcaccgacct gaaccgcgag aagctggttt
     1141 ccccgggaca aacgatcacc gtgttcctgg ggccgttgga accaggggag tataagatct
     1201 tcggcgactt ccaccaggat accgcccgcg gcgtcatggt agcgaagtga cctgccgctt
     1261 cattcttttt gccgcggcgc tggcgctgct ggcaccggag caggcacggg ccgattttca
     1321 cgtgcggtca ccttatgaaa tcgaccttgg ggaactggaa gtcgagcaca acggtgctgc
     1381 gtcgttcgac cgtctgccgg agaaccgtgg cgcacaaagc tacacgattg aatttggcac
     1441 cggcctgacc agttggtggc acagtgagat cgaactcggt ttcgagcgca gtccgggctt
     1501 caaccaatcc acgttggtca cggcggccgt gtccgaaaac acgttccagc tgacagaacc
     1561 tggccagtac tttgccgact tcggattcta cgccgaatat ggccagtcgc tgacaaccgg
     1621 ccgccatgcc gggcctaacg aattcacctt cggcccgttg atcggcaagg atatcggcca
     1681 aacttcacac ttgctgaacc ttttcctcac gcgccaactc ggtcccaatc aggacaatca
     1741 cggccagaca ttcagctatg cctggcaaag ccgctggaac ctgtggcaac cactctcgcc
     1801 ggcgatcgaa atctacggcg atgccggaac gcttggccgc tcgcccaaac tgtcgcaaca
     1861 atcgttgcgc gtcgggccgg ttggcgtggg gtcaatgagc ctgcgcgatc tcggcctcgg
     1921 taatcgagg
//
LOCUS       DASWQN010001271         3376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_519774, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001271 DASWQN010000000
VERSION     DASWQN010001271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519774"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /gene="ggt"
                     /locus_tag="VGC82_21495"
     CDS             <1..1768
                     /gene="ggt"
                     /locus_tag="VGC82_21495"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY0425906.1"
                     /translation="AINDRQAARLLMLAAALLLLSPSSRAASLPPTESATGLVVSAQH
                     LAAEAGVAILRQGGNAIDAAVAVGYAEAVVNPCCGNIGGGGFLTAHLPDGRDIFLDFR
                     ETAPAGASREMYLDAAGQPVHGASLFGWKAVAVPGSVLGLDTALATYGTLPRSIVMAA
                     SIHLARDGFVLGSADADILAHGAKLLRRDPMVARIFLRPDGSPFQAGDRLIQPELAAT
                     LQAIADQGPAAFYQGRIPQAVEAASHSGGGIITAADFAAYRVVETAPLSCTYRGHKIL
                     SAPPPSSGGTTICETLNMLEGYDLHGMGFHAAEAVHVMTEALRLSFFDRNTYLGDPAF
                     VHAPMNRLLSKDYATRLRGLVGERATLSASLGPAVPPVSEKLETTHFSVIDKAGGAVA
                     VTYTLNGAFGSGMMAGGTGFLMNDEMDDFTLKPGAPNQFGLVHGEANAIAPGKRPLSS
                     MAPTIVLKDGAVEMVVGSPGGPRIITATLETILNVIDYGMNAQEAVDAPRLHHQWLPD
                     VLFVEPFALSPDTTALLDQMGYRIQEQKPWGAVALIASGKLGASRPAGAAADSVATRA
                     TEPNMFFGANDPRRPAGVALAP"
     gene            complement(1793..3037)
                     /locus_tag="VGC82_21500"
     CDS             complement(1793..3037)
                     /locus_tag="VGC82_21500"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0425907.1"
                     /translation="MNAPVSHDVLGDPWTMPLDQIDVADARIFQDDVWPDWFARLRRD
                     DPVHFTADSQFGPYWSITKYKDIMKVETNPQVFSSALGITLLDQQTQFRLAMFIAMDP
                     PKHDVQRLVVAPIVAPGNLATMAGTIRERVIKILDGLPRNETFDWVEHVSIELTTQML
                     ATLFDFPFERRRDLTYWSNVATTNFKDPNALVKTEEERFEELKKMAAAMMGMWNERVN
                     TPGGNDLISMLAHGEATRNMDPMEFMGNCVLLIVGGNDTTRNSISGGLWALNKHPDEY
                     QKLRENPGLIPNMVPEIIRWQTPLSYMRRTALEDYELGGKTIKAGDKLAMWYISGNRD
                     PDAIENPDQFIIDRARPRQHLSFGFGIHRCVGNRLAELQLTILWEEIMKRFPNIELVE
                     EPQRVFSSFVHGISSMMVRIPG"
     gene            3244..>3376
                     /locus_tag="VGC82_21505"
     CDS             3244..>3376
                     /locus_tag="VGC82_21505"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY0425908.1"
                     /translation="MHEAEALLALCRSKGIMLAIAECCTGGLIAAALTALAGSSDVLD
                     "
BASE COUNT          587 a         1087 c         1013 g          689 t
ORIGIN      
        1 cgccattaac gatcgacaag cggcgcgcct tttgatgctg gcggcggcac tccttctgct
       61 gtcgccctca agccgtgcgg cctctctgcc ccccactgaa tcagccactg gtctcgtcgt
      121 atccgcgcaa catctagcgg ccgaggctgg ggtcgcgatc ctccgtcagg gcggcaacgc
      181 gatcgatgcc gctgtcgcgg tcggttacgc cgaggcggtg gtcaacccct gttgcggcaa
      241 tatcggcggc ggcggtttcc tgacggcgca tcttccggat ggccgcgata ttttcctgga
      301 tttccgggaa accgcgccgg ctggggccag tcgggagatg tacctggacg ctgccgggca
      361 gccagtgcat ggcgccagtc tttttggctg gaaagctgtc gcagttcccg gttccgtcct
      421 cggtttggat actgcgcttg cgacgtacgg cacactgccg cgcagcatcg tcatggcggc
      481 ttccattcac cttgcccggg acggttttgt gctgggctcg gctgatgccg atatcctggc
      541 ccacggtgcc aaactgcttc gccgggatcc gatggtcgcg cgcatcttcc ttcgtccgga
      601 tgggtctcca ttccaggccg gggaccggct gattcaacct gagctcgccg cgaccttgca
      661 ggctatcgcc gaccagggtc ccgccgcgtt ctaccagggc cgcattccgc aagcggtcga
      721 agccgcgtcc cattcagggg gcgggatcat aaccgccgcc gattttgccg cgtatcgcgt
      781 cgtcgaaacc gcacccttat cctgtactta ccgaggccac aagatcttgt ccgcgccgcc
      841 gccttcgtcc ggcggtacaa cgatctgcga aaccctcaac atgctggagg gctacgatct
      901 ccacggcatg ggctttcacg ccgcggaagc tgtccatgtg atgaccgagg ccctgcgcct
      961 gagctttttc gaccgcaaca cctatctggg cgacccggcc ttcgtgcatg cgccaatgaa
     1021 ccggctgctg tccaaggatt atgcgacccg cctgcgcggc ctggtcggcg aacgagcgac
     1081 cctatcggcc agcctgggtc cggcggtccc gccggtcagt gagaagctgg agaccacgca
     1141 tttctcggtt atcgacaagg ccggtggcgc tgtcgccgtg acatataccc ttaatggcgc
     1201 gttcgggtct gggatgatgg ccggcggcac cggcttcctg atgaacgacg aaatggacga
     1261 cttcacgctc aagccgggcg cccccaacca gttcggcttg gtgcatggcg aggccaacgc
     1321 aattgccccc ggaaagcggc cactgtcctc catggcgcct acgatcgtgc tgaaggacgg
     1381 cgcggtggaa atggtcgtcg gatcaccagg agggccgcgc atcatcaccg cgacgctgga
     1441 aacgatcctc aacgtgatcg attacggcat gaatgcgcag gaggcagtgg acgcgccccg
     1501 cttgcaccat caatggctgc cggatgtgct gttcgtcgaa ccttttgcct tgtcgccgga
     1561 taccacggcg ttgttggacc agatgggata ccgcatccag gagcagaagc cctggggtgc
     1621 cgttgctctg atcgcctccg gcaagctggg cgcttcgaga ccagccgggg cagcggcgga
     1681 ctcggtggcc acccgcgcaa cggagcccaa catgtttttc ggtgcaaacg acccgcgccg
     1741 tcccgccggt gtcgcgctgg cgccttgaac agtcggccga accctgttgc tcctaccccg
     1801 gaatgcgcac catcatcgac gaaatcccat gcacaaaact ggaaaagacc cgctgcggct
     1861 cttccaccag ttcgatgttc gggaaccgct tcatgatttc ctcccatagg atcgtcagct
     1921 gcagttccgc caggcggttg cccacacacc ggtgaatgcc gaatccaaac gacagatgct
     1981 gccgaggccg agcccggtcg atgatgaact ggtcggggtt ctcgatcgca tcgggatcgc
     2041 gattgccgga gatgtaccac atcgccagct tgtcgccggc cttgatcgtc ttgccgccta
     2101 gctcatagtc ctcaagcgcg gtccgccgca tatacgacag tggcgtctgc cagcgaatga
     2161 tctccggcac catgttcgga atcaggccgg gattttcccg tagtttttga tactcgtccg
     2221 gatgtttgtt cagtgcccac aacccgccgc tgatcgagtt ccgcgtggtg tcgttgccgc
     2281 cgacaatcag cagcacgcag tttcccatga actccatcgg gtccatgttc cgcgtcgcct
     2341 cgccatgggc gagcattgag atcaggtcgt tcccgccggg cgtattcacc cgctcgttcc
     2401 acatgcccat catcgccgcg gccatttttt tcagttcttc aaagcgctct tcctcggtct
     2461 ttaccagcgc gttcgggtcc ttgaagtttg tcgtcgcgac gttcgaccag taggtcaggt
     2521 cgcgccgccg ctcgaacggg aaatcgaata gcgtggcgag catctgggtg gtcagctcga
     2581 tcgacacatg ctcgacccaa tcgaaggttt cgttgcgcgg cagtccgtcc aggatcttga
     2641 tgacccgttc gcggatggtg ccggccatcg tcgcgaggtt ccctggtgcg acgatcggcg
     2701 ccaccaccag ccgctgcaca tcgtgctttg gcgggtccat ggcgatgaac atcgccagcc
     2761 ggaactgggt ttgctggtct agcagtgtga ttccgagcgc cgaggagaag acctggggat
     2821 tggtctccac cttcatgatg tccttgtatt tggttatcga ccagtacggc ccgaactgac
     2881 tgtccgcggt gaaatgaacc ggatcatcgc gccgtaaccg cgcgaaccag tcgggccaga
     2941 cgtcgtcctg aaaaatccgt gcatccgcca cgtcgatctg atcgagcggc atggtccagg
     3001 ggtcgcccaa tacatcgtgg ctgaccggcg cgttcatgtt tagcctcctg tggcagcatg
     3061 cggcagaata gaggggcgtg tcgaacgccg caaatatcaa ttgatcggct gcctgccgga
     3121 taggttggcc ggatcgagcg ccatccgatc acgacaaaat ccgcctagtg gacagtcgtc
     3181 ccatcacgga tcacgctcca agccggatac tgaaacggac ccacgatgct tccagatacc
     3241 acaatgcacg aggccgaagc cttgcttgcc ctgtgcaggt ccaagggcat catgctcgcg
     3301 atagcggaat gctgcaccgg cggcctgatc gccgcggcgc ttacggccct tgccggatcg
     3361 tccgatgtgc tggatc
//
LOCUS       DASWQN010001272         4438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_257063, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001272 DASWQN010000000
VERSION     DASWQN010001272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257063"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1659)
                     /locus_tag="VGC82_21510"
     CDS             complement(127..1659)
                     /locus_tag="VGC82_21510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEY0425909.1"
                     /translation="MAESDIAIDIVTSPSGKPEVRQSQTTVFTPRPRLAARALRLIGL
                     DATVTASSAFLCFAMFHPARQFASSPVVIPMACLTVCLILSFTERGLYAPAEIVSRRL
                     PWPKLAWAWLQAVAIGTLVTLCVAAISSALAPAATSSGFDRMGTVLRGVWLPAFLLVG
                     FCALASARLLRLRFHAGPEPLNRTVIIGEGEQVDDLLFRIQAAAHRSFDVIGIVGQAA
                     DTSARRSTPELPSLGDIDVLERLIRQDGVDTVLVALPWSAGEQMRAIIDRISMAPVDV
                     YIYPGMNGLNLPLRRADKAFDLPLLMACTRPIGGWGALIKRAEDIALSLALLLFISPV
                     MLTIAAAIKCSSRGPVFFRQRRLGYNNRVIEILKFRSMYTHLSDADATQQSLRGDKRV
                     TAIGGWLRRTSLDELPQLLNVLKGEMSLVGPRPHALATTAGGLALEEAVPVYASRHRV
                     KPGITGWAQVNGYRGALDSVDKIVHRVNHDLYYIENWSLCLDLKILWRTARLVFADDN
                     AF"
     gene            complement(2178..3290)
                     /locus_tag="VGC82_21515"
     CDS             complement(2178..3290)
                     /locus_tag="VGC82_21515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0425910.1"
                     /translation="MIAPLGQDVLPAEVRARFVDGVNGLRVHLVEAGDPGNPCILLLH
                     GFPELAYSWRKVMPALAGAGYHVIAPDQRGYGRTTGWDADYDGDVGSFRIMNLVRDTV
                     GLLAAMDRPFVTAVVGHDFGASVAAWCALLRPDVFHRVVLMSAPFSGPPGLVQEARSA
                     DIHRALAALPRPRKHYQWYYATREANGDMTRCRQGVHAFLRGYYHHKSADWPGNRPFR
                     LEGWTAGELARMPTYYVMDRDQDMAQTVAREMPRPEAVAACDWLPERELRVYSGEYQR
                     IGFQGGLQWYRCRTQGIGIAELQVFHGRTISVPSLFIAGSRDWGIYQTPGAVERMRAS
                     ACTDLRGCHLLDDAGHWVQQEKPIEVIASLLTFFRG"
     gene            complement(3287..>4438)
                     /locus_tag="VGC82_21520"
     CDS             complement(3287..>4438)
                     /locus_tag="VGC82_21520"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0425911.1"
                     /translation="PYFERLRREDPVHFRKDGMYGSFWSVTKFKDIMTVETHPEIYSS
                     DAKLGGISITDRPMEFRRSSFISMDPPRHDEQRKVVSPIVAPANLNNMSGIIRERVCR
                     VLDGLPRGETFDWVERVSIELTTLMLTTLFDFPEAERSKLTYWSDCATADVNAGGIVD
                     SEEKRLEILAEALRTFTGMWHERAARPPGFDLISMLAHGEATKDLVNDPQEFLGNLTL
                     LIVGGNDTTRNSMSGGLWALCKHPAEYQKLRDNPGLITSMVPEIIRWQSPIVHMRRTA
                     IADAELGGKQIKAGDKVVMWYVSGNRDEDSIDNPNEFIIDRARPRHHLSFGFGVHRCV
                     GNRLAEMQLIILWEEIMKRFPRIELVEEPKRIYSNFIHGISEMQVRIPA"
BASE COUNT          905 a         1371 c         1351 g          811 t
ORIGIN      
        1 aatcgtcagt tcggggttcg atagcctgtt cacgtgggct tctccaaatc cagatctggc
       61 ttcaatcgaa atgaccgggc ctgtgctcag gtcgcaagcg tagaggtctc tgaacgccct
      121 gcgaaatcaa aacgcattgt catcggcgaa tacgagcctc gcggttcgcc acaaaatctt
      181 gaggtcgagg cataatgacc agttctcgat gtaataaaga tcgtgattca ctcgatgaac
      241 aatcttatct acgctgtcca gcgccccgcg gtaaccattg acctgcgccc agccggtgat
      301 gcctggcttg acacgatgac gtgaagcata caccgggacc gcctcctcca gcgccaagcc
      361 gccggcggtg gtcgcgaggg cgtgaggacg cgggccaacc agcgacattt cgcccttgag
      421 cacgttcagc aactgcggca attcatccaa gctggttctg cgcaaccatc cgccgatggc
      481 cgtcacgcgc ttgtcgccgc gaagcgactg ctgcgttgcg tcggcgtcgg acaaatgcgt
      541 gtacatcgac cggaacttca ggatttcgat cacccggttg ttatatccaa gccggcgctg
      601 gcggaagaag accgggcctc gtgaactgca cttgatggcg gccgcgatcg tcagcatcac
      661 gggcgagata aaaagcagca gagcgagcga cagcgcgatg tcttcggcgc gctttatcaa
      721 ggctccccag ccgccgatcg gccgtgtgca tgccatcagc agcggcaggt cgaatgcctt
      781 gtcggcacgg cgaagcggca ggttcaggcc gttcataccg ggatagatat agacatcgac
      841 cggtgccatg gaaattcggt cgataatggc ccgcatctgt tcgccagccg accacggcag
      901 cgcgaccaac acggtatcca cgccgtcctg gcggataagg cgttccagca catcgatatc
      961 cccgagcgaa ggcagttcgg gagtactgcg ccgcgctgac gtatcggccg cctgaccgac
     1021 aattccgatc acgtcgaaac tgcgatgcgc ggctgcctgg atacgaaaca gaagatcgtc
     1081 gacctgctcg ccctcgccga tgatgacggt gcgattgagc ggttccggcc cggcatgaaa
     1141 tcgaagccgc agcagccggg cagatgccag cgcgcagaag ccgacgagca ggaatgccgg
     1201 cagccacacc ccgcgcaaca cggtgcccat gcgatcgaat ccggagcttg ttgccgccgg
     1261 cgccagcgcg ctggatatcg ccgccacgca tagggtgacc aacgtgccga tcgcgaccgc
     1321 ctgaagccat gcccaagcga gcttcggcca gggaagccta cggctgacga tctccgctgg
     1381 cgcatagagg ccgcgttcgg tgaagctcag gatcaggcag accgtcaggc acgccatcgg
     1441 gatgacaacc ggcgacgatg cgaactgcct tgcggggtgg aacatcgcga agcaaaggaa
     1501 ggcggaactg gcggtgaccg tggcgtccag gccgatcagt cgcaatgcgc gggctgccag
     1561 gcggggacga ggggtgaaaa cggttgtctg gctttgccga acctccggtt tgccggaagg
     1621 cgatgtgacg atgtcgatgg ctatatccga ttcagccatt cctggcatca gcctgttcct
     1681 gtcctgtgat aaccatcatc acgaatgtgc ccacgagtat gcgaccgcgc gcgggttcgt
     1741 cgggtcgata ccgatcagcc tattcacaac agatgtcgcc tggttttcgt ctgcgaagta
     1801 cttcaggttc cgggtgatcg agaagtccat ccggacatag gaaggctaac gccctgggaa
     1861 gagcgtacga aaccaaattc tacgtgtacc gcgctgacgt gaaacgtttc ggcataaatc
     1921 agagcgctca attgcgtgag caggcaactg agaaaaatac ggctgtttga tggcgggatc
     1981 acccatatca gcacctcatg ttcggatctt gcaccgtatg tgcacaatca cgacacaata
     2041 aacgtggtta tgtattgtct ggttgcaatg cacaaagtag gtattgtatc aaaacgaggt
     2101 tactatacgt gcaattagtt cagcaaccgc gatcggccat caggcccgac ggcggcgcca
     2161 cccgccggca caggtgatca gccccgaaaa aacgtcagca atgaggcgat gacttcaatt
     2221 ggtttttcct gctgcaccca atgcccggca tcatccagca gatggcagcc tcgcaagtcc
     2281 gtgcaggcac ttgcccgcat gcgttcgacc gccccgggag tctggtagat accccagtca
     2341 cgggagccag cgatgaacag ggagggaaca ctgatcgtgc ggccgtggaa cacctgaagc
     2401 tccgcgatgc cgatcccttg cgtgcggcaa cggtaccact gcaggccgcc ctggaagccg
     2461 atccgttggt attcgccact gtaaacacga agttcgcgct ccggcagcca gtcgcacgcc
     2521 gcgacggcct cgggccgcgg catttcccgc gcaaccgtct gcgccatatc ctgatcccga
     2581 tccatgacat agtaggtcgg catccgcgcg agttcgccag ccgtccatcc ttccaggcgg
     2641 aagggcctgt tgccgggcca atcggcgctt ttgtgatggt agtagccgcg caggaaggca
     2701 tgcaccccct gtctgcaacg cgtcatgtcg ccattggcct cgcgcgtcgc gtagtaccac
     2761 tgatagtgct tgcgcgggcg cggcagcgcc gccagggccc tgtggatgtc cgcggagcga
     2821 gcttcttgca caagccccgg cggaccgctg aacggcgcgc tcattaatac cacgcggtgg
     2881 aagacatcgg gccgcaacaa cgcgcaccag gcagcgaccg aggcgccaaa gtcgtgcccg
     2941 accaccgcgg ttacgaacgg gcggtccatc gcggccagca gcccgacggt atcgcgtacc
     3001 aggttcatga tgcggaagga gccgacatcg ccatcgtaat ccgcatccca gccggtggtg
     3061 cggccatagc cgcgctggtc gggggcgatg acatggtagc cggcccctgc caacgccggc
     3121 atgaccttgc gccaggaata cgccagctcc ggaaagccat gcagcagcag aatgcaaggg
     3181 ttgcccggat cgccggcttc taccaggtga acgcgcagcc cgttgacgcc gtcaacgaag
     3241 cgggcgcgaa cctccgccgg caggacgtcc tgaccgagcg gagcgatcat gccgggatgc
     3301 gcacttgcat ttcggagatg ccgtggatga agttggagta gatacgcttc ggctcttcca
     3361 ccagctcaat tctcgggaag cgcttcatga tttcttccca caggatgatc agctgcattt
     3421 cggccaggcg attgccgacg cagcggtgca cgccgaagcc gaaggacagg tggtgccgcg
     3481 gacgagcccg gtcgatgata aattcgttag gattatcgat cgagtcttca tcgcgattgc
     3541 ccgagacata ccacatcacg accttgtcgc cggccttgat ctgcttgccg ccaagttcgg
     3601 catcggcgat cgcggtgcgc cgcatatgca cgatgggcga ctgccagcgg atgatctcgg
     3661 ggaccatgct ggtgatcagg ccggggttat cgcgcagctt ctggtactcc gccggatgct
     3721 tgcacagcgc ccacaggccg ccgctcatgg aattgcgcgt ggtgtcgttg ccgccgacga
     3781 tcagcagggt cagatttccc aggaactcct gcgggtcgtt gacaaggtcc tttgttgcct
     3841 cgccatgagc aagcatggaa atcaggtcga acccgggcgg acgggcggcg cgttcgtgcc
     3901 acatgccggt aaaggttcgc agcgcctcgg ccaggatctc gaggcgtttt tcctcggaat
     3961 ccacgatccc gccggcgttc acgtcggccg tggcacaatc ggaccagtag gtcagtttgc
     4021 tccgctccgc ttccgggaag tcgaacagcg tggtcagcat cagcgtcgtc agctcgatgg
     4081 acacgcgttc cacccagtcg aacgtctcgc cgcgcggcag cccgtccagc acccggcaca
     4141 cccgctcccg gatgatgccg gacatgttgt tcaggttggc cggcgcgacg attgggctga
     4201 ccaccttgcg ctgctcatcg tgccgcggtg ggtccatcga gatgaagctc gaccgccgaa
     4261 attccattgg acggtcggtg atcgagatgc cgcccagctt cgcgtccgac gaatagatct
     4321 ctggatgggt ctccaccgtc atgatgtcct tgaacttggt caccgaccag aaggagccat
     4381 acatgccgtc cttgcggaaa tggaccgggt cctcccgccg cagccgctca aaatacgg
//
LOCUS       DASWQN010001273         1632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2499181, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001273 DASWQN010000000
VERSION     DASWQN010001273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2499181"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VGC82_21525"
     CDS             <1..916
                     /locus_tag="VGC82_21525"
                     /inference="COORDINATES: protein motif:HMM:NF020683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose oxidase-like domain-containing
                     protein"
                     /protein_id="HEY0425912.1"
                     /translation="QDGRIFFSGGRMDDPMNAQPGIFDLTRNPVPFDPVPDLLAPVRR
                     NQSASVILPPAQDQRVMVSGGGPPEKPDKTHATDQVSIVDLKVPHPAYAEAMPMALAR
                     MHLNLVILPDRTVFVSGGSLKQESEPVSRLQSELFDPATGTWRLMATATVTRLYHSTA
                     LLLPDGRVVAAGGNPEGGKSVRFEPPDPQEEMRLEVFSPPYLFRGPRPVIGAVQDQWR
                     YGQNVTIASPQAGAIKWISLVRNGVTTHSFDNGQRLIDIPITSRTGTELQARVPDNGN
                     LAPPGWYMVFLVNQQGVPSVAKWVHLQA"
     gene            1150..>1632
                     /locus_tag="VGC82_21530"
     CDS             1150..>1632
                     /locus_tag="VGC82_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY0425913.1"
                     /translation="MSGNAGQAWSGKIGMTIKDSVPGWPEPMQPPKGAPNILVVLFDD
                     TGFADFGCYGSPIKTPTIDRLASEGLRYTGFHTTAMCSTTRSALLTGRNHHSVGVGCL
                     ANFDSGFPGYRGKIAKEAGTLAEMLRPHGYRNYMLGKWHVTPLTESGATGPFDGWPLG
                     R"
BASE COUNT          306 a          532 c          504 g          290 t
ORIGIN      
        1 tcaggacgga cggattttct tttccggcgg gcgcatggac gacccgatga acgcccaacc
       61 cggcatcttc gacctgaccc gtaatcccgt tccgttcgat ccggttcccg acctgctggc
      121 cccggtccgg cgcaaccagt cggccagcgt cattctgccc ccggcacagg atcaaagggt
      181 catggtcagc ggcggcgggc cgccggagaa gcccgataag acgcacgcca ccgaccaggt
      241 cagcatcgtc gacctcaaag tgccgcatcc ggcctatgcc gaggccatgc ccatggcact
      301 ggcccgcatg cacctcaatc tggttattct cccggaccgg acggtctttg tcagcggcgg
      361 ctcgctgaag caggagagcg aaccggtttc ccgtctgcaa tcggagctgt tcgacccggc
      421 caccggcacc tggcgattga tggcgacggc gacggtgacg cggctgtatc actcgaccgc
      481 cctgctgctg ccggatggcc gtgtggtggc cgccggcggc aatcccgaag gcggtaagtc
      541 ggtccggttc gagccgcccg acccgcagga agaaatgcgc ctggaagttt ttagtccgcc
      601 ctacctgttc cgtggaccac ggccggttat tggtgcggtt caagaccaat ggcgctatgg
      661 gcagaacgtc acgatcgcct cgccccaggc tggggcaata aagtggatca gcctggtgag
      721 gaatggcgtc accacgcatt ccttcgacaa cggacagcgc ctgatcgaca ttccgatcac
      781 gtcccgaacc ggcaccgaac ttcaggccag ggttcccgac aacggcaacc tcgcgcctcc
      841 ggggtggtac atggtgttcc tggtgaacca gcaaggcgtg ccgtcagtcg ctaaatgggt
      901 gcatctccag gcttagcgtc tggcgcgacg caacggcttc gggtgcggtc cgtcatggtg
      961 gtccgtcaag gtgaacgaag gctgcagggt tcgatgatcg tgtcatggcc gtgcttggcg
     1021 tggccattca cgactttatc ctggcaagca aagtcgtgag ttggtggtgc acgaccgccg
     1081 ggttgcggga acagcggcga tgggtccaac atcgccgcgg caacgaaaag caaccgggga
     1141 acacagtcca tgagcggaaa tgcaggccag gcatggtccg gcaagatcgg catgaccatc
     1201 aaggactcgg tccccggttg gcccgaaccg atgcaaccgc caaaaggcgc gcccaacatt
     1261 cttgtcgtgc tgttcgacga caccggcttt gccgatttcg gctgctatgg ctccccgatc
     1321 aagaccccga ccatcgaccg cctggcctcc gagggccttc gctacaccgg ctttcatacc
     1381 accgcgatgt gctccaccac acgttcggcc ctgctgaccg gacgcaatca tcattcggtc
     1441 ggcgtcggct gtctcgccaa cttcgacagc ggctttccgg ggtatcgcgg aaagatcgcc
     1501 aaggaagccg gcacgcttgc cgagatgctt cggccgcacg gctatcgaaa ctacatgctg
     1561 ggcaaatggc atgtgacacc gctgaccgaa agcggcgcca ccgggccctt cgacggctgg
     1621 ccactcgggc ga
//
LOCUS       DASWQN010001274         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1313148, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001274 DASWQN010000000
VERSION     DASWQN010001274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313148"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VGC82_21535"
     CDS             complement(<1..271)
                     /locus_tag="VGC82_21535"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0425914.1"
                     /translation="MIYRRHFLTLAALSALFPLSVRAQSTDKAAAFVKATGDKLVAVV
                     NGRGSASAKRAQMTQVINSDVDVDGIGRFCLGRYWRQATPEQQKQY"
     gene            complement(303..1127)
                     /locus_tag="VGC82_21540"
     CDS             complement(303..1127)
                     /locus_tag="VGC82_21540"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HEY0425915.1"
                     /translation="MRLVRPFRSSVFGTPGLLAILAIFTLGLAGCATRPPADDPDAVA
                     DYVQTDDPLEPTNRVFYAVNNGLDTVILRPAALAYRYVVPGPVRQGVHNVLANLGTPV
                     QLTNDILEAKPRRAGDTTMRFLINTTAGVLGIFDVASKWGYPEHDADFGMTLATWGVP
                     EGPFLFLPVLGPSNPRDASGFGADIVLDPFTWIGPKHDPGLRAFGWTRYGLNAVDARE
                     RVLDALDQIKKTALDPYATFRSLYRQHRRAQIEELRNDNRATVPVWFPQPVKTSAQ"
     gene            1368..2312
                     /gene="metF"
                     /locus_tag="VGC82_21545"
     CDS             1368..2312
                     /gene="metF"
                     /locus_tag="VGC82_21545"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEY0425916.1"
                     /translation="MDNASATLERWLTGQRFSALPTQAAGHPAPKLSFEFFPPRTEAL
                     EQQLWTCIERLAPLAPRFVSVTYGAGGTTQARTHATVARIARETKLVAAAHLTCIGAT
                     REEVDKVARAYWDAGIRHVVALRGDPPPGMAYEPHPGGYRYASDLVAGLRNVADFEIS
                     VAAYPEVHPGASSPMADLENLKRKLDAGATRAITQYFFDTATYLRFLDLCLAAGIKAP
                     IVPGIMPVSNFAQAVKFSDMCGASVPKWMERLFEGTENDPETRRMIGMVVAAEQVRLL
                     QANGVDEFHFYTLNRPDLTFAIAHVLGVRATSGTRASL"
     gene            complement(2384..>2871)
                     /locus_tag="VGC82_21550"
     CDS             complement(2384..>2871)
                     /locus_tag="VGC82_21550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY0425917.1"
                     /translation="GLQFWGDGADDFSSIPTGTSVASSVITVGNLGSYSFSSLDGSFA
                     AAPSIIIGGSTYTSAVVGTSGSLAAGSESLSLYIVGTFTPAGDLSGFDPNNASETISF
                     TETGITNGAKPKFGSFSVSATFASPAAASPVLTNAPEPASAMVLGAGLLGLGAFRRRK
                     A"
BASE COUNT          558 a          859 c          909 g          545 t
ORIGIN      
        1 ggtattgctt ctgctgctcg ggcgtcgcct ggcgccagta acgtcccagg caaaagcggc
       61 cgatcccatc gacgtcgaca tcgctgttga tgacctgggt catctgcgca cgctttgcgc
      121 tcgctgaacc gcggccgttc accaccgcga caagcttatc gcccgtcgcc ttgacgaagg
      181 ccgcagcttt gtcggtactc tgcgcacgaa ccgagagtgg aaacaacgct gacagggcag
      241 cgagggtcag gaagtgccgg cgataaatca tcgagtgttc cgagctccgg tagcgaaatc
      301 gtttattgcg cggacgtctt tactggctgc gggaaccaga cgggaactgt cgcgcgattg
      361 tcgttacgca attcctcaat ctgagcgcgc cggtgctgac gataaagact gcggaatgtt
      421 gcataagggt cgagtgccgt cttcttgatc tggtccaggg catcaagcac ccgctcccgc
      481 gcatcaaccg cgttcaaccc ataccgtgtc cagccgaacg ccctaagccc gggatcatgc
      541 ttcggaccga tccaggtgaa tgggtcaagc acgatatctg ccccaaaacc cgacgcgtcg
      601 cgcgggttgc tcgggccaag aaccggcagg aacaggaatg gaccttccgg cacgccccaa
      661 gtcgccaagg tcatgccgaa atcggcgtca tgctcgggat agccccactt ggaggcgacg
      721 tcgaagattc ccagcactcc ggccgtcgta ttgatcagga agcgcattgt cgtgtcgccg
      781 gcgcggcgtg gtttagcctc gaggatgtcg ttcgtgagct ggaccggcgt gccgagattg
      841 gccagcacat tatgcacgcc ctgccggacc gggcctggca cgacgtagcg gtaggccaac
      901 gcagctggcc gcaggattac cgtgtcaaga ccattgttga ctgcgtagaa taccctattg
      961 gtcggctcca gcgggtcgtc ggtctgaacg taatcggcca ccgcatcggg atcgtccgcc
     1021 gggggccggg tcgcacaacc tgccaaaccc agcgtaaaga ttgccaagat ggccaatagt
     1081 cccggtgtgc cgaaaaccga ggagcggaat ggacggacta ggcgcatcga cttcctcgga
     1141 ccagttgcgg acacggttgt aaggcgcgcg catccgaacc gccagacact tgcggacaca
     1201 cacatggcac gttccacccg aaatggaaca cccggtactg agttttccgt gatttgaatt
     1261 gtcggcaccg gcttatgacg cgacggaagg caaacgtacc gctcgatgaa tttccgtcct
     1321 ggtgagattc ccttgcgttt cggctcgaaa ccggagattc agcgccgatg gacaatgcat
     1381 ccgccaccct tgaacgttgg ctgaccggcc agcgcttcag cgccctgcca actcaggccg
     1441 ccggccatcc ggcgcccaag ctgtcgttcg agttctttcc gccgcgaacc gaagcgctgg
     1501 aacagcagct atggacgtgt atcgagcgcc tggcaccgct ggcaccgcgc ttcgtttccg
     1561 tcacctatgg cgccggcggg acaacgcagg cccggaccca tgccacggtt gcgcgaatcg
     1621 cccgggaaac gaagctggtt gcggcggcgc acctgacctg tatcggtgcg acgcgcgagg
     1681 aggtggacaa ggttgcccgt gcctactggg acgccgggat ccgtcatgtc gtcgccctgc
     1741 ggggcgatcc gccgccgggg atggcttatg agcctcatcc ggggggctac cggtatgcgt
     1801 ccgacctcgt tgccggatta agaaatgttg ccgacttcga aatttccgtg gcggcctatc
     1861 ccgaggtgca tccaggcgcg tccagtccga tggccgattt ggaaaacctg aagcgcaagc
     1921 tggatgctgg ggctacgcgg gcgatcaccc agtacttttt cgacaccgcg acgtatttgc
     1981 gctttttaga tctgtgtttg gccgcgggca tcaaagcgcc gatcgtgccg gggatcatgc
     2041 cggtgtccaa cttcgcgcag gctgtaaagt tctccgacat gtgcggggcg agtgtcccga
     2101 agtggatgga gcggctgttc gagggtacgg aaaacgatcc tgagacgcgt cggatgatcg
     2161 gcatggtggt cgcggcggag caggtgcggt tgttgcaggc aaacggcgtg gatgagtttc
     2221 acttctacac gctgaaccgt cctgatctga cgtttgcgat cgcacatgtg ctaggggttc
     2281 gggcgacgag cggaacccgg gcttcgcttt aaaggttcga cgtcgccggc aacctcaaca
     2341 tgcccggatg gcgcccctca tgagtcgcca cactttcgct ggttcaggcc ttgcgccggc
     2401 ggaatgcgcc cagcccgagc agacccgcgc ccagcaccat cgcggatgcc ggctctggcg
     2461 cattcgtgag aacaggtgag gcagccgcag gggatgcaaa cgttgcgctg acggaaaagg
     2521 agccaaactt gggcttcgcg ccgttggtga tgccggtttc ggtaaaggat atcgtttcgc
     2581 tggcgttgtt cggatcaaac cccgaaagat caccggccgg ggtgaacgtg ccgacgatgt
     2641 agagcgaaag gctttccgaa ccggccgcca atgatccgga tgtcccgaca accgccgagg
     2701 tgtacgtgct gccgccgatg atgatgctcg gcgcggcggc gaagctgccg tcgagcgacg
     2761 agaaactata tgatccaagg ttgccgacgg tgatgacgct gcttgcgacg ctggttcctg
     2821 tgggaatgct ggaaaaatca tcggcaccat cgccccagaa ttgcaggccg a
//
LOCUS       DASWQN010001275         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1708118, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001275 DASWQN010000000
VERSION     DASWQN010001275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708118"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VGC82_21555"
     CDS             <1..458
                     /locus_tag="VGC82_21555"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0425918.1"
                     /translation="LGKEGNGWNQVTSELAFERSAPDRFLVLFQMLREMVRMAGPNPD
                     RATAAALGKLIAQLATLRSMSLSIAGMLHEGLQPNNESAVVKDLGTRYEQDIPVVARQ
                     LFPTEPELEAVDAYVSMIARSTMNAPRFSIQGGTREILRGIIARGLGLR"
     gene            472..1557
                     /locus_tag="VGC82_21560"
     CDS             472..1557
                     /locus_tag="VGC82_21560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0425919.1"
                     /translation="MAIRDETRMLLESMNRLFEDKSTKQVIDRAETGVFADDLWHAVA
                     ETGVPLAALPESAGGADAEWADLFAVLRVAGRFSAPIPLAETMLAGWVAASAGLELSD
                     GPMTVGPVRGSDRLSIVRDGNGWRLSGKASRLPYAAHATKSILIADGPDGEMAVALEG
                     TGEADVTPGKNIANEPRDTLGFSGIRLSADQVGALGKGVSRGALYRRGALARATMMSG
                     ALERAMDLAVGYAQERVQFGRPISKFQAVQQNLAVLAGQTAAAVAAANLGIEALGKTD
                     PERETFLIAVAKTRVGEAATLACEIAHQVHGAIGFTKEYALQLSTRRLWSWREEFGPD
                     PEWAARVGSFACANGADGLWEMLTAAV"
BASE COUNT          290 a          494 c          567 g          273 t
ORIGIN      
        1 tacttggcaa ggaaggcaac ggctggaacc aggtaaccag cgaactggcg ttcgagcgca
       61 gcgcccccga ccgcttcctg gtgctgttcc agatgctgcg cgaaatggtc cgcatggccg
      121 gacccaaccc ggaccgcgcc accgccgccg cgctgggcaa gctgatcgcc cagctcgcga
      181 ccttgcgcag catgtcgctg tcgatcgccg gcatgttgca cgaggggtta cagccaaata
      241 acgaatccgc ggtggtcaag gacctgggca cccgctacga gcaggacatc ccggtcgtcg
      301 cccgacaact gtttccgacg gaaccggagc ttgaggcggt agatgcctat gtctcgatga
      361 tcgcccgctc cacgatgaac gcgccgcgct tttcaattca gggtggcaca cgcgaaatcc
      421 tgcgcgggat catcgcccgc ggccttggtc tgcgctgaag gaatacttga catggccatt
      481 cgcgatgaaa cccggatgct gctggaaagc atgaaccggc tgttcgagga caaatccacc
      541 aagcaggtca tcgaccgggc ggagaccggg gtattcgccg acgacctgtg gcatgcggtt
      601 gccgaaaccg gcgttccgct ggcagccctt ccggaatccg ccggcggtgc cgatgccgag
      661 tgggccgacc tgttcgcggt gctgcgtgtc gcaggccgct tctcggcgcc gattccactg
      721 gctgaaacga tgctggcggg ctgggttgcc gccagcgccg gcctagaact ttctgacggc
      781 ccgatgaccg tagggccggt tcgcggttcg gaccggttga gtatcgtccg tgatggcaat
      841 ggctggcggc tgtccggcaa agcaagcagg ctgccgtacg ctgcgcacgc gacaaaatcg
      901 atcctgatcg cggacggccc ggatggcgag atggcggttg cgctggaggg caccggcgag
      961 gcggatgtca cgccaggcaa gaacatcgcc aacgagccca gggacacgct gggcttcagc
     1021 gggatacgcc tgtccgccga ccaggtgggg gcgttgggca aaggcgtgtc ccgcggtgcg
     1081 ctgtatcgcc gaggcgcact cgcccgagcg acgatgatgt cgggggcatt ggagagagcg
     1141 atggatctgg cggtgggtta tgcgcaggag cgcgtgcagt tcggccgccc gatctccaag
     1201 tttcaggcgg tgcagcagaa cctcgcggtg ctggccgggc agacggcggc agcggtcgcg
     1261 gcggccaacc ttggcattga agcgctggga aagaccgatc ccgagcggga aacctttctg
     1321 atcgccgtgg ccaagaccag ggtaggggag gcggcgacgc tggcctgcga gatcgcccat
     1381 caggtccatg gcgcgatcgg cttcaccaag gagtacgcat tgcagctctc cacccgccgt
     1441 ctctggtcgt ggcgcgaaga gtttggcccc gatccggaat gggccgcccg tgtcgggtcc
     1501 tttgcgtgcg cgaatggcgc ggacgggctg tgggagatgc tgacggccgc tgtttgacgg
     1561 cgaggccatg atctggggac gacatcccaa aggtgttcgg atgccgaatt ggacaaaaac
     1621 ctct
//
LOCUS       DASWQN010001276         3401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_782878, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001276 DASWQN010000000
VERSION     DASWQN010001276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782878"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VGC82_21565"
     CDS             <1..302
                     /locus_tag="VGC82_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425920.1"
                     /translation="LVAHQRVNQQDLSSKPVGILAAVQKGPSKRAGATVGQEAGAFVC
                     VVDNAKIRYKPAEKRFHCDHLLLAASVCPTMRWLGGDHFASTAGSDCAGHIVVRA"
     gene            complement(416..1495)
                     /locus_tag="VGC82_21570"
     CDS             complement(416..1495)
                     /locus_tag="VGC82_21570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0425921.1"
                     /translation="MNFRNGLVAATILALPVAANAQPLTGLYLGGGAGVNIMQNETAK
                     LNTPIPGVQSSPRLNSRVGPAVVLSLGYGLGNGLRAEVEGSYRYNNFSGTSGQTTSGG
                     LEQKFGAMFNVLYDFGAIIPIVKPYVGVGAGYQWAKESNLHASGLGLAAAAKTGTEGS
                     FAYQGILGVDLPIAAAPGLTFAVDYRFMGLAQNRNYSGVVNTPGSVGRAATFKLSDDY
                     NHTVLIGFRYAFGATAPAPVAPAPAAVAPSPVSRSYLVFFDWDKAALTDRARQIVSEA
                     AANSTKVQYTQLEVNGYTDTSGTPKYNQGLSVRRAQAVAAELVKDGVPKSAIAIQGFG
                     ETHLLVPTGEGVREPQNRRVEIIIR"
     gene            complement(1666..1758)
                     /locus_tag="VGC82_21575"
     tRNA            complement(1666..1758)
                     /locus_tag="VGC82_21575"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1722..1724),aa:Sec,seq:tca)
     gene            complement(1711..3207)
                     /gene="selA"
                     /locus_tag="VGC82_21580"
     CDS             complement(1711..3207)
                     /gene="selA"
                     /locus_tag="VGC82_21580"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEY0425922.1"
                     /translation="MSRSVLSLGMCMARYAPLGGARTMGAMAESSSPLPSVDALLRAA
                     ESQVLLNRFGRSAVTATLRDVLSAKRAAGAFGASAADIINEAADHLVVRFARSQRPVF
                     NMTGTVLHTNLGRAPLPPEAADAAAEALRNATTLEFDLATGRRGERDDHISGLLCELT
                     GAEAATVVNNNAAAVLLVLNTLALGREVPVSRGELIEIGGAFRVPDIMARSGAILKEV
                     GTTNRTHARDYVEAIGPATGALMRVHQANYAIVGFTAMVPIETLGGIARHAGVPLIED
                     LGSGSLVDLERWGLPHEPTPRESIAAGATVVTFSGDKLLGGPQAGLIVGHKNAIAAIN
                     RNPLKRALRLDKGRLAALEVLLRMYLDPDRIAQLVPALRLLTRPQAEIRATAERVGSA
                     ISARWPGRQIVIEACRSQIGSGALPVDLLPSAAVTIGGNALDNIIEYLRRLPRPVIGR
                     VARGHLILDCRCLERADEEPFIAQLSQAADTASGGEAAPVGRPDFKPG"
     gene            3173..>3401
                     /locus_tag="VGC82_21585"
     CDS             3173..>3401
                     /locus_tag="VGC82_21585"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0425923.1"
                     /translation="MHIPKLRTERLILRGLRASDWDAYAAINADPVVRDWLGGNLLSR
                     EQSWAQMETFLGQWALRGYGIFAVQVDGNLAG"
BASE COUNT          657 a         1107 c         1058 g          579 t
ORIGIN      
        1 acctggttgc gcaccagcgc gtcaaccagc aggacctgtc gtcgaaacct gtcggcatcc
       61 tcgccgccgt tcaaaagggt ccaagcaagc gagccggggc caccgtcggt caagaggccg
      121 gcgcgttcgt atgcgtcgtg gacaacgcca agatccgcta caagcccgcc gaaaagcgct
      181 tccactgcga tcatctcctg ctggcggcat cagtttgccc caccatgcga tggcttggcg
      241 gtgaccattt cgcctcgacc gccggcagcg attgcgccgg ccacatcgtg gtccgagcgt
      301 gatctagtca tcgccccgcc ggcaaggcag ggaacacgat gtatagagag aagatgtgac
      361 caacgaaaag gggcgcccga aggcgccccc aatccctgca tccgaaaagc gaagtctatc
      421 ggatgatgat ctcgacgcgc cggttctgcg gctcccgcac gccttcgccg gtgggaacca
      481 gcagatgggt ttcgccgaag ccctggatcg cgatggcgct cttcggcacg ccatccttga
      541 ccagttccgc ggcaacagcc tgcgcacgcc gcacggacag gccctggttg tactttgggg
      601 tgcccgacgt gtcggtatag ccgttgacct caagctgcgt gtactgaacc ttcgtggagt
      661 tcgcagccgc ttccgagacg atctggcggg cccgatcggt cagagctgcc ttgtcccagt
      721 cgaagaacac caggtaggag cgcgacaccg gagacggcgc gacggcggcc ggggccgggg
      781 ccacgggagc aggcgcagtc gcaccgaaag cataccggaa gccgataagc actgtgtggt
      841 tatagtcgtc gctcagtttg aaagtcgcag cacggccgac cgaaccgggg gtgttcacca
      901 cgccgctgta gttgcggttc tgggccagtc ccataaagcg ataatcgacc gcaaaagtca
      961 gaccgggtgc ggcagcaatc ggcaggtcca cacctagaat tccttgatag gcaaagctac
     1021 cttcggtccc ggtttttgca gcggctgcca gtccaagtcc ggaagcgtgc agattactct
     1081 ctttcgccca ctgataaccg gcaccaacac caacatacgg cttcacaatt ggaataattg
     1141 cgccaaagtc gtacagcacg ttgaacatcg cgccaaactt ctgttccaaa ccacccgagg
     1201 tggtttgtcc tgacgtgccg ctaaagttgt tgtagcggta actaccttcc acctcagccc
     1261 ggagcccatt gcccaggcca tacccgaggc tcaggacgac cgcaggccca accctggagt
     1321 ttagacgcgg actggattgc acgccgggga tcggggtgtt caacttagca gtttcgttct
     1381 gcatgatgtt cacgccagcg ccaccgccga gatagaggcc ggtaaggggc tgggcgttcg
     1441 ccgcgacagg cagtgcgagg atggtggccg ctaccaggcc attgcggaag ttcatataac
     1501 tctccttagt tcaacagacc gggcgaagca ggtccgaaaa tttccgccga cccgcctttt
     1561 cgctgctaca accatagcga ccggatatgg cgatagcgag aggcgtcgca acgatcgtgc
     1621 cacactgttg ctgaacagcc acaacacgtc ccacccaagc gtgggcggaa gagagcagga
     1681 gtcgaacctg cccaggacca cgatgcagcc ctaaccgggt ttgaagtccg gccgacccac
     1741 cggggccgct tctcctccgg atgcagtatc ggcggcttgc gataactggg caatgaacgg
     1801 ctcctcgtcg gcacgttcaa gacaccggca atccaggatc aggtgaccgc gggcgacccg
     1861 gccgatcacc ggccgcggca gccggcgaag gtattcgatg atattatcca atgcgttacc
     1921 gccgatcgtc acggcggcgg acggcagcag gtcaaccggc agggcgcctg atccgatctg
     1981 gctgcggcag gcctcgatga cgatttggcg ccccggccac cgcgccgaaa tagctgaccc
     2041 aacgcgctcg gcggtcgctc ggatctcagc ctggggccgg gtcagcaagc gtaaggcggg
     2101 cacaagctgt gcaatccggt caggatccag gtacatccga agcaagactt ccagcgccgc
     2161 caaacgcccc ttgtcgagac gcagcgcccg cttcaacgga tttcggttga tcgcagctat
     2221 cgcgtttttg tgaccaacga tcagcccggc ttgcgggccg cccagcagct tgtcgcccga
     2281 gaaggtaacg accgtggctc cagcggcgat cgactcgcga ggggtcggtt cgtgcggcaa
     2341 cccccaccgc tccagatcca ccagactgcc actgccgaga tcctcgatca gcggaacacc
     2401 ggcatggcgc gcgatcccgc ccaatgtctc gatcggcacc atcgccgtga agccgacgat
     2461 cgcgtagttg gcctgatgga cccgcatcag cgccccggtt gccggaccga tcgcctcgac
     2521 atagtccctg gcatgcgtcc ggttggtggt gcccacctcc ttcaggatcg cacccgaccg
     2581 ggccatgatg tccggcaccc ggaacgcgcc gccgatctcg atcagttccc cgcgcgagac
     2641 cggcacctcc ctgcccagcg ccagggtgtt cagcaccagc agcaccgcgg cggcgttgtt
     2701 gttgaccacc gttgccgcct cggcgccggt cagttcgcac aacaggccgc tgatgtggtc
     2761 gtcccgttcg ccgcggcggc cggtcgcgag atcgaattcc aaggtcgtgg catttcgcag
     2821 cgcctcggcg gccgcgtcgg ccgcttcggg cggcaatggt gcccggccga ggttggtatg
     2881 cagcacggtg ccggtcatgt tgaatactgg ccgctgggaa cgggcgaacc gcacgaccag
     2941 atgatcggct gcctcgttga tgatgtcggc tgcactcgcg ccaaatgcgc cggccgctcg
     3001 cttggcggac aagacgtcac gcagggttgc cgtgacggcg gagcggccga agcggttcag
     3061 cagcacctgg ctttcggccg cgcgcagcag ggcatcgacg gacggcagcg gactggaact
     3121 ttcggccatg gcgcccatcg tacgcgcgcc acccaaagga gcatagcgtg ccatgcacat
     3181 tcccaagctg aggaccgagc ggctcatatt acgcggcctt cgagcgagcg attgggatgc
     3241 ctacgccgcc atcaacgcgg atcccgtcgt gcgagactgg ctcggcggca atctccttag
     3301 ccgcgagcag tcatgggcac agatggaaac gttcctggga cagtgggcac tgcgaggcta
     3361 tggcatcttt gcggtgcagg tcgacggcaa tctggcgggg c
//
LOCUS       DASWQN010001277         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1840935, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001277 DASWQN010000000
VERSION     DASWQN010001277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840935"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VGC82_21590"
     CDS             complement(<1..530)
                     /locus_tag="VGC82_21590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0425924.1"
                     /translation="MKLGVFDHMDRGLVPLNRFYEERLKLVEAYDRAGFYGYHVAEHH
                     ATPLGVAPAPGIWLAAVAQRTRQLRFGPLVYLLPLYHPIKLLEEICMLDQISGGRLML
                     GVGRGISPIELRFYGLDPDETPAMYAEALEVIQRGMTSPILNFQGKYYNYRDVPMEMT
                     PFQTPHPPLWYGLARA"
     gene            complement(716..1126)
                     /locus_tag="VGC82_21595"
     CDS             complement(716..1126)
                     /locus_tag="VGC82_21595"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0425925.1"
                     /translation="MLKRRGFVSCAICTAVGLVASSVEAGAQGTPGLSRTILQKTEYP
                     GDKHVSILVMAEIDPNFFVARHTHPGVESSYLVAGAGTLSVKGQPDRALKAGDGFQIP
                     PEVPHSLRNGPEKTRIAITYIVEKDKPLASSAPE"
     gene            complement(1197..>1576)
                     /locus_tag="VGC82_21600"
     CDS             complement(1197..>1576)
                     /locus_tag="VGC82_21600"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0425926.1"
                     /translation="VPANMRQGLPQDDILRRGCAIDAVQARSLIGCKDIVLVDLREKT
                     ERQKHGSIPGSLHAPYSNLEENIQPGGLLFELASVANRRLLFYCAFGERSAMAVCAAH
                     DAGLETACHIHGGIDAWKKAGSA"
BASE COUNT          300 a          502 c          473 g          301 t
ORIGIN      
        1 tctgcccggg caaggccata ccacagcggc ggatgcggtg tctggaacgg cgtcatctcc
       61 atcggcacat cccgataatt gtagtacttg ccttggaagt tcaggattgg gctggtcatt
      121 ccgcgctgga tcacctccag cgcctcggca tacatcgccg gtgtctcatc ggggtcgagc
      181 ccatagaagc gcaattcgat gggagagatg ccgcgcccga cacccagcat cagacgcccg
      241 ccgctgatct gatccagcat acagatctcc tccagcagct tgatcgggtg atacagcggc
      301 aggagataga ccagtggccc gaaacgaagc tgcctggtcc gctgcgcgac cgcagccagc
      361 cagatgccgg gtgccggtgc gacacccaac ggcgtggcat gatgttccgc cacatgatag
      421 ccgtagaaac cggcgcggtc gtaggcttcg accagtttca gccgctcctc gtagaagcgg
      481 ttcagcggca cgagacctcg gtccatatgg tcgaagacac caagcttcat gatggcgcca
      541 ttgtaatgtt tcctctccag gtctggagtc gtactcgcgg ccccggcagt tgtccagttt
      601 ggccggtcga gcagctttga cagacggcgg tcggatgtag ccgagacgaa agggtgtgga
      661 ccacattgct tccgccgcgg ccaccaaccg ctcgggtcgc ttcaggaccg gcaggttact
      721 ccggcgcgga cgacgccaac ggcttatcct tttcgacaat gtaggtgatg gcgatgcggg
      781 tcttctccgg gccattgcgc aagctgtgcg gtacttccgg tggaatctgg aacccgtcgc
      841 cggccttcag ggcgcgatcg ggctgtccct tcaccgacag ggtgcccgcg cccgcgacca
      901 gatagctcga ctcgacgccc ggatgcgtgt gccgggcgac aaagaagttc gggtcgatct
      961 ccgccatgac caggatcgag acatgcttat caccgggata ttcggtcttt tgcaggatag
     1021 tgcgagagag tcccggcgtg ccttgcgcac ccgcctcaac cgaagaagcg accaagccga
     1081 ccgccgtaca aatcgcgcac gatacgaaac cgcgtctctt gagcattttt gttccctccc
     1141 gtttttttcc gctcccgact ttaggggcga tgcgacgcgt gtccagtccg aaccggctag
     1201 gccgaccccg ctttcttcca cgcatcgatg ccgccatgga tatggcaggc ggtttccagt
     1261 cccgcgtcgt gcgcggcgca cacggccatg gccgaacgct cgccgaaggc acagtagaac
     1321 aggagccggc ggttggcgac cgaggcgagt tcgaacagca agccgcccgg ctgaatattc
     1381 tcttccaggt tcgaatacgg cgcatgtaac gaaccaggaa tcgatccatg cttttgccgc
     1441 tcggtttttt cccgcaggtc aaccagcacg atgtccttgc agccaatcag cgaccgcgcc
     1501 tgaacggcat cgatggcgca accacgacgc aggatgtcgt cctgcggaag gccctgccgc
     1561 atgttcgccg gcaccg
//
LOCUS       DASWQN010001278         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_783139, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001278 DASWQN010000000
VERSION     DASWQN010001278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783139"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VGC82_21605"
     CDS             <1..1160
                     /locus_tag="VGC82_21605"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY0425927.1"
                     /translation="LRFGGLDLTVVLRTPALAALRPLLSPNLPALTNVRFDGRVVVPE
                     NAASIAFKNAKLLTREGDVTGDWTLGQQATLMMDGKLVSPRLDMDLMLAAFGVELPPA
                     PAFDGNMGPVISIAPLPWALLRGPVVSLSARTEAMTFQGQVWNNVDFVLDLNGGRLRI
                     SPLTLSLPNGQLRMSMAVDASRDTVPVSLEMHAPGIPLALVARYAGLPGPMDGAVRVD
                     AVLHATGLSPHDLAASLDGTVSAILVGGRMTNAAFIMLTSASLEALGISVPAQGETTL
                     RCLGLVGSFTKGVGLLPTIALDTTYLQVDGTGQVDLAQETVAFKLHPLAQISGSAIAV
                     PVVVEGPFHAVKGRLDANGIDKLGLFIDSLFGTDRSTACIDAGFVPGGKPG"
BASE COUNT          299 a          541 c          563 g          343 t
ORIGIN      
        1 cgcttcgttt tggcggtctg gatcttacgg ttgtgttgcg gacgccggcg ctcgcggcac
       61 ttcggccgtt actgtcgccg aacctgccgg cgctgacaaa cgttcgtttc gatggacggg
      121 tcgttgttcc cgagaacgcc gcatcgattg ccttcaagaa cgcgaagctg ctgacccgtg
      181 agggggacgt gaccggagat tggacgcttg gccaacaagc gacgctgatg atggatggca
      241 agctcgtgtc cccgcggctc gacatggacc tgatgcttgc ggcgttcggg gtcgaattgc
      301 cgcccgcacc tgcttttgat ggcaacatgg ggccggtcat ttcgatcgcg cccctgccct
      361 gggcactgct gcgcgggcca gtcgtcagcc tttcggccag gaccgaagcg atgacatttc
      421 aaggtcaggt ctggaacaac gttgacttcg tactcgatct caacggcggg cggctgcgga
      481 tttctccact aacgctttcg ctcccgaacg gtcagctgcg gatgtccatg gccgttgacg
      541 cgtcgcgcga tacggtgccg gtcagcctgg agatgcatgc cccgggtatc ccgctcgccc
      601 tcgtcgcgcg ttacgcaggg ctgcccggtc cgatggatgg ggccgtccgg gtcgatgcgg
      661 tgctccacgc aaccgggctg agtccgcacg acctcgcggc gtcattggat ggtacggtct
      721 ctgcgatcct cgtcggcggc cgcatgacga acgcggcctt catcatgttg acgtcggcat
      781 cgctggaagc gctggggatt agcgtgcctg cacaagggga gaccacgctg cgttgcctcg
      841 gcctggtcgg ctcgttcacc aagggcgttg gccttctgcc gaccattgcg ttggatacga
      901 cctaccttca ggtggacggc accggccaag tcgacctcgc ccaggagacc gtcgcgttca
      961 agctgcaccc gctggcccaa atttcgggtt ccgcgatcgc ggtgccggtt gtcgtcgagg
     1021 gaccgttcca tgcggtgaag ggacggctcg atgccaatgg catcgacaag ctgggattgt
     1081 tcatcgacag cctgtttggc actgatcgtt cgactgcgtg catcgacgca gggtttgtgc
     1141 ctggcggcaa gccagggtga gaaggcccga taggccaccc tgggatgaag tccgatacga
     1201 cacagagcct gctgacgaag cgcgatccga agcgccaacc aagggtggtg aaggcatgtc
     1261 agctttccgc gctctcgacc gatcagccga cagcccgggt tcggccacac cgcggcttgg
     1321 cagatcattc gctcattcgt ggggcacgcg gtgatcccgt agacggtcgt gcctggccat
     1381 ccgagaccat cgcaagaata cgcccggtgg caatccaatg aggttgtatt agcaacattc
     1441 ccaaaccgtc tcccgatcct tgcggcctgc ccggttgaag cgatagcgtt gacagcgacg
     1501 gaacggtcag ccgcaagtaa acgcttgatc gacgggaagc gccgcggatt ttggccggcg
     1561 cgcgccaaag cggggagacg catcatgccg gatacccaca tcctgactga acggccggct
     1621 caggatcagg ccgctaccta cgacgcgatt gtcatcggcg ccggcatttc cggcatgttc
     1681 atgctctacc gcctgcgcga actcggcatg acggcgcggg tctttgaagc cggcaccaac
     1741 gtcggc
//
LOCUS       DASWQN010001279         6226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2633267, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001279 DASWQN010000000
VERSION     DASWQN010001279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2633267"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..913)
                     /locus_tag="VGC82_21610"
     CDS             complement(8..913)
                     /locus_tag="VGC82_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY0425928.1"
                     /translation="MSGEAAREAFLGWMEQERHAAANTVEAYGHALAGFLGFLTQHLG
                     AEPDLAALAALRAADVRAWLAALANRKLVAASRAQHLSALRSFFRFIGRRHGVEIAAV
                     RLVSTARARPALPRALRVEQARDVTDNVGEVSDDPCIQLRDVAFFSLLYGSGLRIAEA
                     LGLNVRDAIALRTNPSLTVIGKGSKTRIVPVLPVVRTAIDAWLAVHPAVQPETALFLG
                     ARGKRLDPAVAQKVLRTYRRLAGLPEHATPHALRHSFATHLLASGSDLRSIQDLLGHA
                     SLSTTQRYTTVDDTALLAVWRKTHP"
     gene            complement(1086..1967)
                     /locus_tag="VGC82_21615"
     CDS             complement(1086..1967)
                     /locus_tag="VGC82_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0425929.1"
                     /translation="MDVLRGNNGPRYRHKPENAAPYETITVDKLTPIIGAEIGGVDLS
                     APLPNHQMDEVHRALAENCVIFFRDQTLTQEQHLSFGRNFGNLHLHPAAPHAPGHPEL
                     MIIHADKDSPRANGEGWHSDVSCDPEPPMGSILYIKQCPPRGGDTLFASMYAAYEALS
                     DRMKAYLDGMVALHDGEESYRGTYANLGVADKPVYPRAEHPVIRTHPVTGRKALYVNR
                     GFTRRLIGVPRDEGAAVLAYLYGHAENPLFQCRFRWQENSVAFWDNRCVQHRAMWDYW
                     PHTRSGFRVTVAGDKPV"
     gene            2316..4175
                     /locus_tag="VGC82_21620"
     CDS             2316..4175
                     /locus_tag="VGC82_21620"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HEY0425930.1"
                     /translation="MPQPTTESGTLASQESSARPTRDTAPSPQATAEMRPRKAVLNDW
                     VRALENTQRRRASPITLLSALRAVADRQGGEPALIGLHEQLTYDELAQRANRYARWAL
                     DQGLKPAEIVCLLMPNQPDYVAIWLGLTQVGCVVALLNTHLTVDALAHSIRESGASHV
                     IAAGSLMGNARAVVGQLPAATMWWAHGAGDATMPRIDLAVASHSGHELDVHEQRSPSP
                     HDCALLIYTSGTTGLPKAAKVTHSRIMEWSDWFAGMMDAQRNDRLFNCLPMYHSTGGV
                     VAIGSMLVTGGSVVIRERFSASRFWGDLADTDCTIVQYIGELCRYLIQAPPHPHETSH
                     RVRLFCGNGLRGEVWPTLQQRFGIPRILEFYAATEGGVSLYNCEGKPGAIGRVPAFLA
                     HRFPVTLIRCDPETGIPQRDAAGRCVPCGPDEPGEAIGQLDADGTLRRFDGYTNKAAS
                     REKVLHDVFMPGDSWFRTGDLMRKDASGYYYFVDRLGDTFRWKGENVSTTEVASVLNA
                     CPGVIEAAVYGVTVPGTEGKAGMAALAIDNAFSLSAFRAHLHANLPAYARPLFIRICD
                     NIETTGTFKLKKSELVRQGYTDTANDPTWFDDRISGAFVKCDAGFLAQNGLKL"
     gene            complement(4191..4913)
                     /locus_tag="VGC82_21625"
     CDS             complement(4191..4913)
                     /locus_tag="VGC82_21625"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0425931.1"
                     /translation="MRNIVVTGASRGVGLEIAQTLVRSGFRVIAIARSKTEALGAAMA
                     AQADQLHFHSGDLADLAALPDLARSLRGRFGPVYGLVNNAGIGTAGILSTMPDTAIEY
                     LVRLNVVSPMTLTKYLVRPMMAARSGRVVNISSIVGSTGYSGLAAYSASKAALVGFTR
                     SLARELGPLGITVNAVAPGFLETDMTHDLDAKQREQIRRRSALHRMANPNDVAAAVDY
                     LISDKAANITGTVMTVDAGNTA"
     gene            complement(4906..6207)
                     /locus_tag="VGC82_21630"
     CDS             complement(4906..6207)
                     /locus_tag="VGC82_21630"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEY0425932.1"
                     /translation="MVFGGLVEGSCLGVPPETLHGRSVLIACQDQLPAVLAAIELDGI
                     ARRILLCPPDLPASCLPAILTDAQVDAIAWDGVSAAGREADGQSVIACGSELQPRSSA
                     QASRSVETEWVLFTSGTTGQPKMVRHTLTSLSGPIDDGLVVASKAVWSTFYDVRRYGG
                     LQILLRALIGGGSMVLSRAEEPAGEFLLRAGKSGVTHISGTPSHWRRTLMSSFGHLMS
                     PAYVRLSGEVADQAILDQLRRAYPASSVAHAFASTEAGVAFDVRDGLAGFPADYIGQP
                     GAKVEMRVEDGTLRIRSSRTSTGYLGDHDRALRGPDGFVDTGDIVERRGDRYYFNGRR
                     EGVINVGGLKVYPEEVEAVINQHPAVRMSRVWARSSPITGALVAADVVLQANQESFAT
                     LRAAIIDHCRRGLTAYKVPVSVRQVQSLEIAPSGKLMRHHA"
BASE COUNT         1217 a         1937 c         1918 g         1154 t
ORIGIN      
        1 cctaacttca aggatgagtc ttgcgccaga ccgccagcaa cgcggtgtcg tccaccgtcg
       61 tgtagcgttg cgtcgttgac aggcttgcat gtccaagcag gtcctggatc gagcgcaggt
      121 cgctgccgct ggcaagcaaa tgggtggcga aggagtgccg caacgcatgc ggggtggcat
      181 gttccggcag gccggcgagg cggcgatagg ttcgcagcac cttttgtgcc accgcgggat
      241 cgagccgctt gccccgcgcg cccaggaaca gagccgtttc aggttgcacg gccggatgga
      301 ccgcaagcca tgcgtcgatt gccgtacgca ccaccggcaa gaccggcacg atgcgggttt
      361 tcgaaccttt gccgataacc gtcagcgacg ggttggtccg caatgcgatg gcatcccgca
      421 cgttcagtcc caaagcctcg gcgatgcgca ggccgctgcc atacagcagg gagaagaaag
      481 cgacgtcccg tagctggatg caaggatcgt ccgaaacttc gcccacattg tccgtcacgt
      541 cccgcgcctg ttcgaccctc agcgctcggg gcagggcggg acgtgcgcgg gcagtggaaa
      601 ccagccgcac ggccgcaatc tctaccccat gccggcggcc gatgaagcga aaaaagcttc
      661 gcaaagccga cagatgctgg gcccgcgacg ccgccaccaa ttttcgattg gccaaagccg
      721 ccagccacgc gcgaacatcc gccgcccgca gcgccgccag cgcggccaga tcaggttcag
      781 caccgaggtg ctgtgtcaga aaccccaaga aacccgccag cgcgtggcca taggcttcaa
      841 ccgtgttggc cgcggcgtgg cgttcctgtt ccatccagcc gagaaaggcc tcgcgggcag
      901 cctcgccgct cagggcgggg attggacccg ccgagggcgc ggtggtggga tttcggtttg
      961 aggtatgccg gggcatcagc cggtcgcccg ccggagtgtc gcagcgaaca gagcccggag
     1021 tgtcgctgcg aaaagagaaa aagcgagccc cgccccgacg ttcgcatgtg ctgcggtatg
     1081 agggttcaaa ccggcttgtc gcccgccacg gtcacgcgga accccgaacg ggtgtgcggc
     1141 cagtaatccc acattgcccg atgctgcaca cagcggttgt cccaaaacgc caccgagttc
     1201 tcctgccaac gaaaccggca ctgaaacaac ggattttccg catggccata caagtatgcg
     1261 agcaccgccg caccctcgtc ccgaggcacg ccgatcaatc gcctggtaaa tccgcggttg
     1321 acatagagcg ccttgcgccc tgtcaccgga tgcgtgcgga tcacggggtg ttcggcccgc
     1381 gggtagaccg gtttatcggc gactccaagg ttcgcataag ttccacggta ggattcttcg
     1441 ccgtcatgca gtgcgaccat cccgtccaga tacgccttca tccggtcgga tagtgcctcg
     1501 taggccgcat acatgctggc gaacagcgtg tccccaccgc gcggagggca ctgcttgatg
     1561 tagagaatgc tccccatcgg cggctccgga tcgcaggaga cgtcgctgtg ccagccctca
     1621 ccattggccc gcgggctgtc cttgtcggcg tggatgatca tcaactccgg atggccgggc
     1681 gcgtgcggtg cggcgggatg caggtgaagg ttgccgaaat tgcgcccgaa cgacaggtgc
     1741 tgctcctgcg tcagggtctg atcgcggaag aaaatcacgc agttctcagc gagagcccgg
     1801 tggacctcgt ccatctgatg gttcggcagc ggcgccgaca gatccacgcc accgatctcc
     1861 gcgccaatga ttggcgtcag cttgtcgacg gtgatggtct cgtacggcgc agcattctcg
     1921 ggtttgtggc gataccgagg gccattgttg ccgcggagca cgtccatgat tcggtccttt
     1981 tcctctgacg tttggcggga tcatcggccc cacgtctcgg gatcgcaagt gggacggtac
     2041 ctttgggacg gttaccttct taaacggcaa acctggccgc gtcatgccgc atgatgctcc
     2101 cggcccctgg gaacaccgaa atcgtccggg cctgcgcttg aaaggttcag gccgaagctg
     2161 cgcttcaaag catcgggaaa acggacatct gagcggatac ggctggaatc ttgattggca
     2221 ggcttacgag atcggcctta ctgctaccaa gtgtgatttc actggtaccg agtgtgattt
     2281 cgaggcttcc aagtattgag acgatcaacg gaatgatgcc gcagccgacg acagaatccg
     2341 ggacattggc ttctcaagaa agctcggcac gcccaacccg cgatacagcc ccatcaccgc
     2401 aagcaaccgc cgaaatgcgg cccaggaaag ccgtgctcaa cgactgggtg cgagcgcttg
     2461 aaaacaccca aaggcgacgc gccagcccaa tcacgctctt gtccgctcta cgagcggtcg
     2521 cggatcggca aggcggcgaa ccggcactca tcggactaca cgagcagctg acctatgatg
     2581 aattggctca gcgggccaat cgttatgctc gatgggcgct cgaccaagga ttgaagccgg
     2641 ccgaaatcgt ttgcctgctc atgccgaacc agcccgacta cgtcgccatc tggctcggac
     2701 tgacgcaggt gggttgcgtc gttgccctgc tgaacaccca tctgactgtc gatgcgcttg
     2761 ctcacagcat ccgcgaatcg ggagcaagcc atgtgatcgc agctggatcg ttgatgggca
     2821 acgcccgcgc tgtcgtcggt caacttcccg cggcgacgat gtggtgggcg catggggccg
     2881 gcgacgcgac catgcctcgg atcgacctgg cggtggcatc ccatagcgga catgaactcg
     2941 atgttcatga gcagcgatcg ccgtcgcccc atgactgcgc gcttttgatc tacacctcgg
     3001 gcaccacagg cctgccgaag gccgccaaag tgacccattc gcgtatcatg gagtggagcg
     3061 actggttcgc cggcatgatg gatgcgcagc ggaacgaccg gctgtttaac tgcctgccaa
     3121 tgtatcacag taccggcggt gtcgtggcca tcggatcgat gctggtcacc ggtggttcgg
     3181 ttgtcatccg ggagcgtttt tcggccagcc ggttctgggg cgatcttgcg gacaccgatt
     3241 gcacgatcgt ccaatacatc ggcgaactct gccgctacct catccaggct ccgccgcacc
     3301 cgcacgaaac cagccaccgc gtcaggctgt tttgcggcaa cggcctgagg ggggaagtgt
     3361 ggccaaccct gcagcaacgc tttggtatcc cacggatcct cgagttctac gccgcgaccg
     3421 aaggcggcgt ttcgctgtac aactgcgaag gaaagcctgg tgctatcggc cgcgtgcctg
     3481 cgtttttggc gcaccggttt cctgtgacct tgatccgctg tgaccccgag accgggatcc
     3541 cgcaacgtga cgccgcggga agatgcgttc cctgcggccc agacgaacca ggcgaggcaa
     3601 tagggcagct tgacgcggat ggcacgttgc ggcgcttcga tggctacacc aacaaagcgg
     3661 cttcccggga gaaggtgctg catgacgtat tcatgccagg agacagttgg ttccgaaccg
     3721 gcgacctgat gcgtaaggat gcatccgggt attactactt cgtcgatcgg ctgggcgata
     3781 cgtttcgttg gaaaggtgag aacgtctcta cgaccgaggt tgcctccgtg ctgaatgcct
     3841 gtcctggcgt catcgaagcc gccgtttatg gggtaaccgt gccgggtacc gagggcaaag
     3901 caggaatggc cgccttggcg atagacaatg cattcagctt gtcggcgttt cgcgctcatc
     3961 tccatgcgaa tttgcccgcc tacgcccgtc ctctattcat acggatctgc gataacatcg
     4021 agacgacggg caccttcaag ctgaaaaaga gcgaacttgt tcggcaaggc tacacggaca
     4081 cggcgaacga tccgacctgg ttcgatgaca ggatttccgg cgccttcgtg aaatgcgacg
     4141 ccggctttct ggcgcagaac ggcctgaagc tatagttcgt ccgaaattgt ttaggccgtg
     4201 ttcccggcat cgaccgtcat cacggtgcca gtgatattcg cggctttgtc gctgatgaga
     4261 taatcgactg ccgcggcaac atcgttcggg ttcgccatac ggtgcagtgc gctgcgacgc
     4321 ctgatttgct cgcgctgctt tgcatccaga tcatgcgtca tgtccgtctc gaggaagccg
     4381 ggcgccaccg cgttcaccgt gatgcccaaa ggcccaagtt ctcgtgccag cgagcgggtg
     4441 aagccgacaa gtgcggcctt gctcgcactg taggccgcaa ggccgctgta acccgtgctg
     4501 ccgacgatag acgaaatgtt caccaccctg ccggatcgcg ccgccatcat tgggcgcaca
     4561 aggtatttcg tcagggtcat tggcgaaaca acgtttagac gcaccaaata ctcgattgcc
     4621 gtatccggca tggtcgagag gataccggcg gtgccgatcc cggcgttgtt caccaagccg
     4681 taaaccgggc cgaacctgcc gcggagactt cgcgccagat cgggcaacgc ggcgaggtcc
     4741 gccaaatctc cggaatgaaa atgcagttgg tccgcttgtg ccgccatagc tgcgccaagt
     4801 gcctcggtct tgctccgggc aatggcgatg accctgaagc cggagcgaac cagggtttgt
     4861 gcgatttcga gaccgacgcc gcgactggcg ccggtgacga cgatattacg catgatggcg
     4921 catcaacttg cccgatgggg cgatttccag cgattgcacc tgtcgaacag agaccgggac
     4981 tttgtaggcg gtcaggccgc gccgacaatg gtcgatgatg gcggcgcgca gggtggcgaa
     5041 gctttcctga tttgcctgaa gcacgacatc ggctgcgacg agggctccgg tgatcggact
     5101 gctgcgtgcc catacacggg acatcctgac cgccggatgc tggttgatga ccgcctccac
     5161 ctcctccgga tagaccttca gcccgccgac gttgatcacg ccttccctgc gtccgttgaa
     5221 gtaatatcgg tcgcctcgcc gttcgacgat gtcacccgtg tcgacaaagc catccggacc
     5281 acgaagtgct ctgtcgtgat cacccaagta gccggtggaa gttcgcgacg agcggatgcg
     5341 caaggtgccg tcttcaacgc gcatctccac tttcgccccg ggctggccga tatagtccgc
     5401 aggaaaaccg gccaggccgt cacgaacatc gaaagcgaca ccggcttcgg tcgatgcgaa
     5461 ggcgtgggct acactcgatg cagggtacgc gcgtcgcaat tggtcgagga tcgcctggtc
     5521 ggccacttct cccgagagcc tgacataggc cggtgacata aggtgtccga acgaactcat
     5581 gagtgttcgg cgccagtgcg acggcgtgcc ggaaatatgg gtgacgccgg atttgcccgc
     5641 gcgcagcaaa aattcgccag cgggctcttc ggcgcgcgac aggaccatgg agcccccgcc
     5701 aatcaaagcc cgcaacagga tctgcaggcc gccgtagcgg cgaacgtcat aaaacgtact
     5761 ccagaccgct ttgctcgcga ccacgaggcc atcatcgatt gggccgctca ggctggtcaa
     5821 cgtgtgacga accatcttgg gctgcccagt cgttcccgac gtgaacagca cccactctgt
     5881 ttcgaccgac cggcttgctt gcgcggagga tctgggttgg agttcgcttc cgcaggcgat
     5941 gacagactga ccatccgcct cacgtcccgc tgcgctgact ccgtcccatg cgattgcatc
     6001 gacctgtgca tcggtcaaaa tagcgggcaa gcatgacgcc ggcaaatcgg gtgggcacaa
     6061 cagaatgcgg cgagcgattc cgtccagttc gatcgcggcc agcactgccg gcaactgatc
     6121 ctggcaagcg atcaaaaccg agcggccatg caatgtctcg ggcggtacgc ccagacagga
     6181 accctcgacg aggccaccga acaccacctg tctgggccca tcgctg
//
LOCUS       DASWQN010001280         2639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1575380, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001280 DASWQN010000000
VERSION     DASWQN010001280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575380"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VGC82_21635"
     CDS             complement(<1..267)
                     /locus_tag="VGC82_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEY0425933.1"
                     /translation="MLMAVLGIVYGDIGTSPLYAFKASLQLFDAKATTPTEILGVLSL
                     IFWSLIVIVTLKYVFLVMRADNRGEGGILALMALAQRVSVGRTVK"
     gene            complement(446..1417)
                     /gene="thyX"
                     /locus_tag="VGC82_21640"
     CDS             complement(446..1417)
                     /gene="thyX"
                     /locus_tag="VGC82_21640"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEY0425934.1"
                     /translation="MIHTREQAAEIAEARAHAASTLRPTVPALEAMLFQAVPVLDHGF
                     VRVIDYMGDDTAIVQAARVSYGRGTRRVQEDAGLIRYLMRHRHTTPFEMCEIKFHVKL
                     PIFVARQWIRHRTASVNEYSARYSILDKEFYIPSAEHLAAQSATNRQGRGDVLDGQEA
                     AEVLDLLRSDAERSYANYARMLNEGEAADPARRGLARELARMNLTLNTYTQWYWKTNL
                     HNLFHFLSLRADAHAQYEIRVYADAMTRMTEAWVPVAAAAFRDYRLGAVTLSAQMLVV
                     LRRMLAGETVTQETSGLNRREWVEFEAQFPAQLVGKSADQHPGGTSI"
     gene            1656..>2639
                     /locus_tag="VGC82_21645"
     CDS             1656..>2639
                     /locus_tag="VGC82_21645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003625820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY0425935.1"
                     /translation="MQAQGTMQGQGTMQGYDPASFFCEMLRPGQPQHPTLSLLMTRLA
                     ALPIDSLRRRAAEAERDLLERGITFTVYSDATAIDRILPFDLIPRVITPAEWHQIETG
                     VCQRVRALNMFLYDIYHERKILADKIVPADLVLKNAGYCQAMVGFDVPFNTYVHVCGT
                     DIVRDETGVFRVLEDNARTPSGVAYVVENRHMSLRVMSDLMTSLRVRGVDEYGLRLHN
                     AMAEIAPAGVDDPQVVVLSPGIFNSAYFEHVFLAREMGVPLVEGRDLTVEGGKVWMRT
                     TSGLAEVHTIYRRIGDDFLDPDAFNPDSLLGVRGLIEAWRRGAVAIANAVGT"
BASE COUNT          458 a          824 c          879 g          478 t
ORIGIN      
        1 tttcacggtg cgcccgaccg agacgcgctg cgccaatgcc atcagcgcca ggatgccgcc
       61 ctcgccacgg ttgtcggcgc gcatcaccag gaagacgtat ttcagcgtga cgatgacgat
      121 cagcgaccaa aagatcagcg acagcacccc gaggatttcg gtcggcgtcg tcgctttggc
      181 gtcgaacaat tgcaggctgg ccttgaaggc gtagagcgga ctggtgccga tatcgccata
      241 gacgatcccc agcaccgcca tcagcatgcc caaggtcggc tttggcctgc cgtgctcccc
      301 gggctcccga tcctggctca aatggttgac ctcatgctgt gcgggtccga cgcgtaagac
      361 ccgttccggg cgcgctgtcc ctaccccggc aactggcttg tagcaagcaa cgcacaattt
      421 ccactgtgcc ggcagcttca tccgatcaaa tagaagtgcc cccagggtgc tggtcggcgg
      481 atttgccgac gagctgggca gggaactggg cttcgaactc gacccactcc cggcggttca
      541 ggccgctggt ttcctgcgtg acggtttcgc cggccagcat ccgccgcagc acgaccaaca
      601 tttgggcgga cagcgtgacc gcccccagcc ggtagtcgcg gaacgcggcg gcggcgaccg
      661 ggacccaagc ctcggtcatc cgcgtcatcg cgtcggcata caccctgatc tcgtattgcg
      721 catgggcgtc agcgcgcagc gacaggaagt ggaacaggtt gtggaggttg gttttccaat
      781 accactgcgt gtaagtgttc agggtcaggt tcatccgagc gagttcgcgc gccaagccgc
      841 ggcgggcggg atcggcggct tcgccttcgt tcagcatccg ggcgtaattg gcatagctcc
      901 gttcggcgtc cgagcgcagc aggtccagga cctcggccgc ttcctgcccg tccagcacgt
      961 cgccgcggcc ctggcggttg gtggccgact gtgccgcgag gtgttctgcc gatggaatgt
     1021 agaactcctt gtccaggatc gagtagcgcg cggaatactc gttcacgctg gcggtgcggt
     1081 ggcgaatcca ttgccgggcg acgaagatcg gcagtttgac gtggaacttg atctcgcaca
     1141 tctcgaacgg cgtggtgtgg cggtggcgca tcagatagcg gatcaggccg gcatcctcct
     1201 gcacgcgcct tgtgccacgc ccgtatgaca cccgggcggc ctgaacgatc gcggtgtcgt
     1261 cacccatata gtcgatgaca cggacgaagc catggtccag caccgggacg gcctggaaca
     1321 gcatggcttc cagtgccggc accgtcgggc ggagcgtgga cgcggcatgt gcgcgggctt
     1381 cggcgatctc ggctgcctgt tcccgtgtgt ggataatgtc agccaaggca acctccgcga
     1441 accggccttg ccaatacagc gtggcgcggc cgggaaaaag cggcttttcg tatcgccacg
     1501 gcgaaccgag gaaattgctt tcctgcctgc cggtttaagt tatcatgccg cattgcaaaa
     1561 tggagacacg aatgccggcg ggtggcatga gccagatcca gagccagccg cagccgcgtc
     1621 tgcaggccca ggggacgatg caggcccaag ggacgatgca agcccagggc acgatgcagg
     1681 gccagggcac gatgcaaggc tacgatccgg cttcgttttt ctgcgaaatg ctgcgccccg
     1741 ggcagcctca gcaccccacg cttagcctgc tgatgacgcg gctggcggca ctgccgatcg
     1801 acagcctgcg ccggcgggcg gcggaggctg agcgggatct gctggaacgc gggatcacct
     1861 tcacggtcta ttccgatgcg acggcgatcg accgcatcct gccgttcgac ctgattccgc
     1921 gggtgatcac gccggccgag tggcatcaga tcgaaaccgg ggtctgccag cgtgtgcggg
     1981 cgctgaacat gttcctgtat gacatctacc acgaacgcaa gatcctcgcc gacaagatcg
     2041 tgccggccga tctggtgttg aagaatgccg gctactgtca ggccatggtc ggtttcgacg
     2101 tgccgttcaa cacctacgtg catgtctgcg gcacggatat cgtgcgcgac gagaccggcg
     2161 tctttcgggt gctggaggac aatgcccgca cgccatccgg cgtggcctac gtggtcgaga
     2221 accgccacat gagcctgcgg gtgatgtccg acctgatgac cagcctgcgc gtgcgcggcg
     2281 tcgatgagta cggactgcgg ctgcacaacg cgatggcgga aatcgcgccc gcgggggtcg
     2341 atgatccgca ggtcgtggtg ttgtcgccgg gcatcttcaa ctcggcctat ttcgaacatg
     2401 tcttcctggc gcgcgagatg ggggtgccgc tggtcgaggg acgggacctg accgtcgagg
     2461 gcggcaaggt ctggatgcgg accacctcgg gccttgcgga ggtgcacacg atctatcggc
     2521 gcatcggcga cgatttcctg gatcccgacg cgttcaatcc ggacagcctg ctgggcgtgc
     2581 gcggcctgat cgaagcttgg cgccgggggg cggtggcgat cgccaacgcc gtcggtacc
//
LOCUS       DASWQN010001281         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_125332, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001281 DASWQN010000000
VERSION     DASWQN010001281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125332"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /gene="dxs"
                     /locus_tag="VGC82_21650"
     CDS             <1..1758
                     /gene="dxs"
                     /locus_tag="VGC82_21650"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY0425936.1"
                     /translation="GHQAYPHKILTGRRDRIRTLRQGGGLSGFTKRSESEYDPFGAAH
                     SSTSISAGLGMAVSRDLKSARYAAASETERAAKGLQNDDRNVICVIGDGAMSAGMAYE
                     AMNNAGALHSRLIVILNDNDMSIAPPVGAMSAYLSRLMSSRSFLSLRELAGRMAKRFP
                     RGIERTARRAEEYARGILTGGTLFEELGFYYVGPIDGHNIDHLLPVLRNVREAEETGP
                     ILVHVITQKGKGYAPAEQSADKYHGVSKFNVITGEQAKAPPGPPGYTKVFADALIAEA
                     TKDPTIVAITAAMPSGTGLDRFAKAFPDRTFDVGIAEQHAVTFAAGMATEGMKPFCAI
                     YSTFLQRAYDQIVHDVALQSLPVRFAMDRAGYVGADGATHAGSFDLAYLGCLPNMVIM
                     APSDEAELVHVVATAVAIGDQPSAFRYPRGEGTGVALPSAGVILPMGKGRVMREGNNI
                     AILSLGTRLADALKAADELAARGFPTTVADARFAKPIDTALVEQLARNHEVLIIIEEA
                     AIGGFGARVMHHLAWKGLLDSGLKIRPMVMPDLFIDHDSQPKQLAEAGLSAKDIVAVA
                     LGAVGIEVPRVGSKVVTSR"
BASE COUNT          329 a          601 c          618 g          339 t
ORIGIN      
        1 ggccatcagg cctatccgca taaaatcctg accggccggc gcgaccggat caggacgctg
       61 cgccagggcg gcgggctgtc cgggttcacc aaacggtcgg aaagcgagta cgacccgttc
      121 ggcgcggcgc attcctccac ctcgatttcc gccggcctgg gcatggcggt gtcgcgtgac
      181 ctgaagtcgg cgcgatatgc ggccgccagt gaaaccgagc gggcggcgaa gggtttgcag
      241 aacgatgacc gcaatgtgat ctgcgttatc ggggacggcg cgatgagcgc gggcatggcc
      301 tacgaggcga tgaacaacgc tggtgccctg cattctcggc tgatcgtcat cctgaacgac
      361 aacgacatgt cgatcgcgcc gccggtcggc gccatgagcg cgtatctgtc gcggctgatg
      421 tcgtcgcgca gcttcctgtc gcttcgcgag ctggccggac ggatggccaa gcgctttccc
      481 cgcggcatcg agcgcaccgc ccgccgggcc gaggaatatg ccaggggcat cctgaccggt
      541 ggaacgctgt tcgaggagct gggattctac tatgtcggcc cgatcgacgg gcacaacatc
      601 gatcacctgc tgccagtgtt gcgcaacgtc cgtgaagccg aggagacggg accaatcctg
      661 gtccatgtca tcacccagaa gggcaagggc tatgcgccgg ccgaacagtc ggcagacaag
      721 tatcacggcg tgtccaagtt caacgtcatc accggcgagc aggccaaggc gccgcctggt
      781 cctccaggct ataccaaagt gttcgccgac gcgctgatcg ccgaggcgac aaaggatccc
      841 accatcgtcg ccatcaccgc cgcgatgccg tccggcaccg gactcgatcg gttcgccaag
      901 gcgttccccg atcgcacgtt cgatgtcggc atcgccgaac agcacgccgt gacctttgcc
      961 gccggcatgg caaccgaggg tatgaagccg ttctgcgcga tctattcgac gttcctgcaa
     1021 cgcgcctacg accagatcgt gcacgatgtc gcgctgcaat cgctgccggt ccgcttcgcc
     1081 atggatcgag ccggttacgt cggcgccgac ggtgcgactc acgccggcag cttcgacctt
     1141 gcctaccttg gctgcctacc caacatggtc atcatggcgc cctccgatga agccgaactg
     1201 gtacatgtcg tcgcgacagc ggtcgccatc ggcgatcagc cgagcgcgtt ccggtacccg
     1261 cgtggtgagg gcaccggtgt cgcgttaccg tccgcaggtg tgatcctgcc catgggcaaa
     1321 ggccgcgtca tgcgcgaagg caacaacatc gccatcctgt cgctggggac gcgtctggcg
     1381 gatgcgctga aggcggcgga cgaattggcg gcccgcggtt ttcccaccac ggtcgccgat
     1441 gcgcgtttcg ccaagccgat cgacaccgcg ctggtcgagc aactggcgcg gaaccacgag
     1501 gtcctgatca tcatcgaaga agccgcgatc ggcgggttcg gtgctcgggt gatgcaccat
     1561 ctggcttgga aagggttact cgattcggga ttgaagatcc gtccgatggt catgccggac
     1621 ctgttcatcg accacgattc ccagcccaag cagttggctg aggctggcct ttccgccaag
     1681 gacattgtcg ccgtggcgct gggggccgtt ggaatcgagg tgccccgcgt aggttcgaag
     1741 gtcgtcacct cgcgatgatc gggctgttgg caggggatgc gtttttcggc ttggcggatc
     1801 ggcgtgccgg caccggtcct tttgaattga gccgcgcctg accatgacgg acgggactgt
     1861 tccaagatca gccgcggggc cggctgt
//
LOCUS       DASWQN010001282         5276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2367977, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001282 DASWQN010000000
VERSION     DASWQN010001282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2367977"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VGC82_21655"
     CDS             <1..209
                     /locus_tag="VGC82_21655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY0425937.1"
                     /translation="MAGHVMLEVFGSFIVMLGGLGLLGYFPAALSLGVNTILIGFELL
                     VATLQAYVFAILTCIYLHDAVHLH"
     gene            283..507
                     /locus_tag="VGC82_21660"
     CDS             283..507
                     /locus_tag="VGC82_21660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C family protein"
                     /protein_id="HEY0425938.1"
                     /translation="MDVASAKMLGAGIAMIALAGVGIGMGTIFGSLVTSVARNPASRD
                     RVFGLAILGFALTEAVALYALVIAFIILFV"
     gene            520..1137
                     /locus_tag="VGC82_21665"
     CDS             520..1137
                     /locus_tag="VGC82_21665"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HEY0425939.1"
                     /translation="MKQPDVMQPFAPMKVLRRLAPVLMAGLVVLPSVAMAEGMPQLDF
                     KTPLTISQVVWGAIIFVVLYLLLSRTGLPMVESVLQERAAKISKDLDEARAAKAGADA
                     GMMQADQATAKARAESQSAINAALDEAKKAAAAQSETLNARLERQLQDSEAQIAQARA
                     AAMSALRQVATETAATVVTRLTGTAPDAGRLDSAIGSALAARGIG"
     gene            1153..1668
                     /locus_tag="VGC82_21670"
     CDS             1153..1668
                     /locus_tag="VGC82_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY0425940.1"
                     /translation="MHHETSFFADPRNWVLIAFFLFFILFGRKLWGALSAMLDARAIA
                     VRTEIEEASRLRREAEAMLRDAEKQRADALADAKALVEGARAEANRVTAAAAAEAEAS
                     AKRRERMAIDRIAAAEKAAVDDVRLAAADIATTAARQVLAEGLTADADAVLIDHAISQ
                     LPSALAARRAA"
     gene            complement(1811..2269)
                     /locus_tag="VGC82_21675"
     CDS             complement(1811..2269)
                     /locus_tag="VGC82_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0425941.1"
                     /translation="MQRKSFSDMQCPIARGLEHVGEWWSILILRDAFRGLTRFDEFSE
                     SLGIAPNMLARRLTALVEAGLLRRQRYTDRPPRDEYMLTDRGRDFRPVLLAMLAFGNK
                     HFPPRDREVQIINTETGQRVNPILVDPATGQPVDGPGYQVVSTMLRQTAA"
     gene            2330..3058
                     /locus_tag="VGC82_21680"
     CDS             2330..3058
                     /locus_tag="VGC82_21680"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0425942.1"
                     /translation="MSNCTASVAVVGAGDYIGSAIARRFAREGYHVFAGRRNGEKLAP
                     LVAEIEAAGGVCTGLSLDARQEDGVADFMRQADEAAPLEVAVFNIGANVNFPVLETTE
                     RVFRKVWELACYAGFFTGREAARRMLPHGRGSIFFTGATASVRGGSGYTAFASAKFGL
                     RAVAQSMARELAPRNIHVAHLVIDSGVDTEWVRQRIAARGGSTEDRVLMQPASIAETY
                     WQLHRQPRDAWTHELDVRPSSEPW"
     gene            3069..3671
                     /locus_tag="VGC82_21685"
     CDS             3069..3671
                     /locus_tag="VGC82_21685"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEY0425943.1"
                     /translation="MQVEFHFDFGSPNAYLAHRVIPAIEARTRVKFRYVPVLLGGVFK
                     ATGNRSPAEAFAGIQNKPAYERLETERFLRRHRIEDFARNPYFPVNTLQMMRGAVAAE
                     IDGELERYVNAMFHHMWEAPKKMDEPEVLRDALTASGLDAAAMMARMQDPAVKAKLIA
                     NTEMSVARGAFGSPTFFVGDAMYFGKDRLRDVEEAIQGIG"
     gene            3761..5068
                     /locus_tag="VGC82_21690"
     CDS             3761..5068
                     /locus_tag="VGC82_21690"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425944.1"
                     /translation="MRGIRRREVAAGLFATGIAPGLARAAAPIPQEGIGNNIKHLSYS
                     DQGGRPDGVQVMVNRLHVYVGHMFSNGITVLDATDPAKLKPVGFWTAGDFTRTHHIQV
                     FDDTMLVANGANIVAMQSYDSMRGYFENNLADSITNRKKFRSGLSIHDISKPAELREI
                     AFLEMPGLGINRLWWVGGRYAYVSAHFDGFTDHILCIVDLKDLAKPEIVSKWWLPGMN
                     RAAGETTVAPKGKRWALHHMITAGDLGYAAWRDGGLTIHDVSDPTAPKLLSHINWSPP
                     FPGGTHTPLPLPGRGLAVVADESNADNCAKGIFHTFVLDVRAPQNPVPISTLPTPTDR
                     DFCHLGNFGPHNLHENRPGSFQSEEIIFATYHNAGLRVFNIKDAFAPKEVGYWVPPVP
                     AKLIDPRPNIALAAQTCDAYVSKDGIIYLSDWNAGLHVLEYKG"
BASE COUNT          995 a         1614 c         1691 g          976 t
ORIGIN      
        1 tgatggccgg ccatgtgatg ctcgaggtgt tcggatcgtt catcgtcatg ctcggcggcc
       61 ttggattgct gggctacttt ccggccgctc tttcgctcgg agtcaacacc atcctgatcg
      121 gatttgaact gctggtggcg actctgcagg cttacgtctt tgcgatcctg acgtgcatct
      181 atctgcacga cgcggttcac ctgcactgac gccacttcaa caccacatgg caacggggcg
      241 gacttcttcg ttcgcgcccg gataacgaaa ggaacttgga ctatggacgt cgcttccgcc
      301 aagatgctgg gtgccggcat tgcaatgatt gccctcgccg gggtcggcat cggcatgggc
      361 acgatcttcg gctcgctcgt taccagcgta gcccggaacc cggcctcccg cgaccgcgtg
      421 ttcggcctgg ccatccttgg cttcgcgctg acagaagccg tagcgctcta cgcgctggtg
      481 atagccttca ttattctttt cgtctgatca ggcggcaccg tgaagcagcc tgacgtcatg
      541 cagccattcg cgccgatgaa ggtcttgcgc cggctggctc ctgttctgat ggcagggctg
      601 gtggtgctgc cttcggttgc gatggctgag gggatgcctc agctcgactt caagactcca
      661 ctcacgatca gccaggtcgt ctggggggca atcatcttcg tggtgctgta tctcctgctg
      721 tcccggaccg gcctgccgat ggtggaatcg gtgctgcaag agcgggcggc gaagatctcc
      781 aaggatctcg atgaggctcg cgccgccaag gccggtgccg acgccggcat gatgcaagcc
      841 gatcaggcga ccgccaaggc gcgggcggaa tcccagtcgg cgatcaacgc ggcgttggac
      901 gaagcgaaga aagccgccgc ggcgcagagc gaaaccctga acgcccgtct ggaacggcaa
      961 ctgcaggatt cagaagccca aatcgctcag gcaagagcag cggccatgag cgctctgcgt
     1021 caggtggcga cggaaacggc tgccacggtg gtgacccggc tgaccgggac ggctcccgat
     1081 gccggccggt tggacagtgc cattggatcg gctttggccg cccgcggtat cggctgaaca
     1141 cgggagcgga cgatgcatca cgaaacaagc ttcttcgccg atcccagaaa ctgggtcctg
     1201 attgcgtttt ttctgttttt tatcctgttt gggcggaagc tctggggcgc cctctccgcc
     1261 atgctggatg cccgggcgat cgccgtcagg accgagatcg aggaagcgtc ccggctgcga
     1321 cgggaagccg aggccatgct ccgcgatgcc gaaaagcaac gtgcggatgc cctggctgat
     1381 gccaaggcgc tggtcgaggg cgcacgagcc gaagccaacc gtgtgaccgc cgccgcggcc
     1441 gccgaagccg aggcctctgc taagcgccgt gaaaggatgg cgatcgatcg tattgccgct
     1501 gccgagaaag ccgctgtgga cgatgtccgc ctggccgccg ccgacatcgc gaccactgcc
     1561 gcgcgtcagg tcctagccga aggtctgacc gcggatgcgg atgcggtgct gatcgaccac
     1621 gccatttcgc agcttccatc ggcgctggca gcccggcgcg cggcctaaac aaaaccgacc
     1681 tccagcgtcg caatcgagcc cgccaaggca gtgccatcgc gcaggccctg cgtcgggtcc
     1741 agaccagcca ttcgcgacag gcgtcggcgc gctcagcttc gcatcgtcca actgaaaacg
     1801 cggtcgttga tcatgccgcg gtctgcctca gcatggtgga aacgacctgg taacccgggc
     1861 catccaccgg ctgaccggtc gcgggatcga ccagaattgg attcacgcgc tgtcccgttt
     1921 cggtattgat gatctggacc tcccggtccc ggggcgggaa atgcttgttg ccgaacgcca
     1981 gcatcgccag cagcaccggg cggaaatcgc gtccccgatc cgtcagcata tattcatccc
     2041 gcggcggacg atcggtgtag cgctggcgcc gcaacagccc ggcttccacc aacgccgtca
     2101 gccggcgggc cagcatattt ggtgcgatcc caagactttc agaaaactcg tcgaagcgcg
     2161 tcaggccgcg aaacgcgtcg cgcaggatca ggatgctcca ccactcaccg acatgttcca
     2221 gcccccgagc gatcgggcac tgcatgtcgc tgaaactctt tcgctgcatt tacaagcact
     2281 cgaccattaa ctacgatgtt gatagttagc caggtcggaa ggaacgtcaa tgtcaaactg
     2341 caccgcgtct gtcgctgtcg tcggcgccgg cgactacatt ggcagtgcca tcgcccgccg
     2401 gttcgcccgc gagggctacc acgtgtttgc cggccggcga aacggcgaga agctggcgcc
     2461 cttggtcgcg gagatcgagg cggcgggagg tgtctgcacc ggcctcagcc tcgacgcgcg
     2521 gcaggaagac ggcgttgccg acttcatgcg tcaggccgac gaggcggcac cgctggaagt
     2581 ggcggttttc aacatcggcg ccaacgtcaa tttcccggtg ctcgaaacga ccgaacgcgt
     2641 gttccggaaa gtctgggaac tcgcctgcta cgcgggattc ttcaccggac gggaagccgc
     2701 gaggcggatg ctgccgcacg gaaggggctc gatctttttc accggtgcaa cggccagcgt
     2761 acgcggcggc agcggttaca cggcgttcgc cagcgcgaaa ttcggcctcc gagccgtcgc
     2821 ccagtccatg gctcgggaac ttgcgccgcg gaacattcat gtcgcgcatc tggtgatcga
     2881 ctcaggcgtc gatactgaat gggtccgtca gcgcatcgca gcgcgaggcg gttcgaccga
     2941 ggatcgcgtc ctgatgcagc ctgcgtcgat cgccgaaaca tactggcagt tgcaccggca
     3001 gccgcgcgat gcctggaccc atgaactgga cgtacggccc agcagcgaac cgtggtgagg
     3061 gacgcgtcat gcaagtggaa tttcatttcg acttcggcag cccgaacgcc tacttggcgc
     3121 accgcgtcat tccggcgatc gaggcacgga cgcgggtgaa atttcgctac gttccggtgc
     3181 tgctgggcgg cgtgtttaaa gccaccggca atcgttctcc ggcggaagcg tttgccggca
     3241 ttcaaaacaa gccggcatat gagcgcttgg aaaccgagcg tttcctccgc cggcatcgga
     3301 tcgaggactt tgcgcgcaac ccgtatttcc cggtgaacac gctgcagatg atgcggggcg
     3361 cagtggcagc tgaaatcgac ggggaactgg aacggtatgt caacgcgatg ttccaccaca
     3421 tgtgggaagc gccgaaaaag atggatgaac cggaggtgct ccgcgatgcg ctgaccgcgt
     3481 cgggccttga tgcggcggcg atgatggcgc ggatgcagga tccggcagtg aaggcgaagt
     3541 tgatcgccaa caccgagatg tcggtggcgc gcggtgcctt cggctccccc accttcttcg
     3601 tcggcgacgc gatgtatttc ggcaaggatc ggctgcggga cgtcgaggaa gcgatccagg
     3661 ggatcggctg agcgcgggcg ccaagctccg ttagggtggg tcccattgac ccggacggtg
     3721 cacaaagtca ccatcgcccg ataataaaaa ggggcgtgcc atgcggggaa tcagacggcg
     3781 cgaggtcgcg gccgggcttt tcgcaaccgg catcgcgcct ggcctggcgc gcgccgcggc
     3841 accgatccca caagagggaa tcgggaacaa catcaagcac ctgtcctatt ccgaccaagg
     3901 gggacggccg gatggcgtgc aggtgatggt caaccgcttg catgtctatg tcggccacat
     3961 gttcagcaac ggcatcaccg ttctggatgc gacggatccg gcgaagctga agccggtggg
     4021 attctggacc gccggcgatt tcacccgcac ccatcatatc caggttttcg acgatacgat
     4081 gctggtggcg aacggcgcca acatcgtggc gatgcagtcc tatgacagta tgcgcggcta
     4141 cttcgagaac aatctcgccg acagcatcac caaccgcaag aagttccgtt ccggactgtc
     4201 gatccacgat atctccaagc ccgcagagct gcgcgagatc gcgttcctgg agatgccggg
     4261 gttggggatc aaccggctgt ggtgggttgg cggacgctac gcctacgtgt ccgcccactt
     4321 cgacgggttc accgaccata ttctatgcat cgtggacctg aaggacctcg cgaaaccgga
     4381 gatcgtctcg aaatggtggt tgccggggat gaaccgggcg gccggtgaga ccacggtcgc
     4441 gccaaagggc aagcgttggg cgctgcacca catgatcacc gccggtgacc tgggctatgc
     4501 cgcatggcgc gatggcggtc tgaccattca cgacgtcagt gacccgacgg caccgaagct
     4561 gctttcgcat atcaactggt cgccgccatt ccccggcgga acccatacgc cgctaccgtt
     4621 gcccggacgc ggcttggctg tagtcgcgga cgagtccaac gccgacaact gcgcgaaggg
     4681 aattttccac acgttcgtgc tggatgttcg tgcgccgcag aacccggtgc cgatctcgac
     4741 cctgcccacg ccgacggacc gggatttctg ccatctcggc aattttggcc cacataacct
     4801 gcatgagaac cgacccgggt cgttccaatc ggaggaaatt attttcgcca cctatcacaa
     4861 cgcgggcctg cgggtattca atatcaagga cgcgtttgca cccaaagagg ttggctactg
     4921 ggttccgccg gtgccggcaa agctgatcga tccgcggccg aatatcgcgt tggcggcgca
     4981 gacttgcgat gcgtacgtgt ccaaggacgg gatcatctac ctcagtgact ggaacgctgg
     5041 actgcatgta ctggagtata aaggttaggc cgatgctaat cgcgctacag tccgtgagcc
     5101 gccatcgtcg ctgaaatgac ctgcgcggca tcctttaacc cgagttacgg gtttcttatg
     5161 ggatagaggc cgtgatcggc tttggcggca ggggattgat ctgataggcg acgatcgccg
     5221 ccgtcaggtg agtgaaagca ttgaagacgg agcgatgctt gggcagtcgg agggag
//
LOCUS       DASWQN010001283         3107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_390098, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001283 DASWQN010000000
VERSION     DASWQN010001283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390098"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VGC82_21695"
     CDS             complement(<1..682)
                     /locus_tag="VGC82_21695"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0425945.1"
                     /translation="MSSTTTPSPYLAVRDEWLARRTETIREPELPIVDPHHHLWERPD
                     WRYMLDDLLLDINSGHNIIGTVFVQCRSMHRASGPEAFRPVGETEFVNGVAAMSASGG
                     YGPAKICAGIVGHVDLRNGEKSRDVLEAHIRAGGGRFRGIRHITAYDPDPALMNPAYA
                     PPPGLMYDPTFRAGFACLAPLNLSFDAWLYHPQIGDLTALAQAFPGTPICLNHVGGPL
                     AIGSYEGKR"
     gene            complement(751..2109)
                     /locus_tag="VGC82_21700"
     CDS             complement(751..2109)
                     /locus_tag="VGC82_21700"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0425946.1"
                     /translation="MIPDLVIVGAGPAGLGAATEAVKHGLSVVLLDENAAPGGRIWQG
                     LEARGAKDTEDAAALALIRRFRDSAVEAHWNASVWAIEPDGHVFWSDRDGAAHSVSGT
                     NVLLATGAIERPMPIAGWTLPGVMTVGAAQIALKTGGLLPDHRTWLAGQGPLLLLYAT
                     QVLEAGGWIAGVIDLSDGFAPIRAMRRLSVAALPDLRRGLAWRRQLSQAGVRWIAAFE
                     LRAEAAITTASGTSSAGFGGQAAQTLGGVSFRTATGWQTEPADLLLLHDGVVPSVQIT
                     RALSCVHAWSETQRCWHPVVDDLGRTSVQGILVAGDGAGIGGAAAAVLSGRIAVLGLA
                     GGDASALRAERVRCLAVRPLLDAMFPSRKLHLDDATLVCRCEEVTAGAVRHAARSGCQ
                     GMNQLKAYTRCGMGPCQGRICGPVAIEVLAEAFGVPPSAVEPLRTRFPTKPVSVGALA
                     GL"
     gene            complement(2106..>3107)
                     /locus_tag="VGC82_21705"
     CDS             complement(2106..>3107)
                     /locus_tag="VGC82_21705"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY0425947.1"
                     /translation="WKQDYVETVAWCDMGLYVGCTKTNIPSLPELELGRGVCGVKIFA
                     GSSTGDLMIEDDEHLEKAMRSGRRRIAFHSEDEYRLQERKPMFKSGDPYSYHMVWRDE
                     ECAFLGTRRLMALARKTGRPAHILHVSTAQELEYLRDFRDIATCEVLVNHLTQVAPDC
                     YDRLEGFGVMNPPIRGPEHMEAAWKAINNGTVDTIGSDHAPHPRDKKLLPWPDCPAGL
                     TGVQTLVPIMLDHVNAGRLPLTRMVDLMCAGPARVYGVVGKGRLAAGYDADFTLVDLK
                     RQRRIEESWIAAPCGWTPFAGMQVTGWPIATIVRGQVVMRDDEVLGEPSGRLVKFIG"
BASE COUNT          544 a         1084 c         1005 g          474 t
ORIGIN      
        1 cccgcttacc ctcataagac ccgatcgcca acggcccacc aacatggttc aggcagatcg
       61 gcgtccccgg aaacgcctgt gccagagcgg tcagatcgcc aatctgagga tgatacagcc
      121 acgcatcgaa cgacaggttc aaaggtgcca ggcacgcgaa gccagcgcga aacgtcggat
      181 cgtacatcaa gcccggaggc ggcgcatagg ccgggttcat cagcgcaggg tcgggatcat
      241 aggccgtgat atgcctgatc ccacggaacc gcccgccgcc cgcccgaatg tgagcctcca
      301 gcacatcgcg ggacttctcg ccgttgcgca ggtcgacatg accgacgatg ccggcgcaga
      361 tctttgccgg accataaccg ccggatgcgc tcatggccgc tacgccattg acgaattcgg
      421 tctcgccaac cgggcggaac gcttccggcc cggacgcgcg gtgcatcgac cggcactgca
      481 cgaacacggt gccgatgatg ttgtggccgc tgttgatgtc caacagcagg tcatcgagca
      541 tataccgcca gtccggccgc tcccacagat ggtggtgcgg atcgacgatc ggcagctccg
      601 gttcccggat ggtctcggtg cggcgggcga gccattcgtc gcgaacggcc agatacgggc
      661 tcggggtcgt ggtgctgctc atgccggcgt ttccctcatg tggttgcggc cattgtttcc
      721 cgccggacgc gggctgccaa gagggccaga tcacaaaccg gccaaggccc cgacgctgac
      781 cggcttggtc gggaagcggg tgcgcagggg ctcgaccgcc gacggcggga cgccgaacgc
      841 ctcggccagt acctcgatcg cgaccggccc gcagattcgc ccttggcacg gacccatgcc
      901 gcagcgggta taagccttga gctggttcat cccctggcag ccggagcgtg cggcatggcg
      961 gacggcaccg gcggtgacct cctcgcaccg gcagaccagg gtggcgtcat ccagatgcag
     1021 cttccgggac gggaacatgg cgtccagcaa tgggcgcacg gccaggcatc gcacccgttc
     1081 agcacgcagg gcggaggcat cgcccccggc cagcccaaga acggcgatcc gccccgacag
     1141 caccgccgct gcggccccgc cgatgccggc tccgtcgccg gcgacgagga tcccttgcac
     1201 cgacgtccgg cccaagtcgt cgaccaccgg atgccagcag cgctgcgtct cgctccaggc
     1261 atgaacgcag ctaagcgccc tggtgatttg gaccgatgga acgaccccgt catgcagcag
     1321 caacaggtcg gccggttcag tctgccatcc ggtggcggtg cggaaggaaa ccccgcccag
     1381 cgtctgcgcg gcctgtccgc caaaccctgc agacgacgtg ccggatgcag tggtgatggc
     1441 ggcctcggcc cgcagttcga aggctgcaat ccacctcacg ccagcctggg acagttgccg
     1501 ccgccaagcc aggccgcggc gaaggtccgg cagtgccgcc accgacagac gccgcattgc
     1561 ccggatgggc gcgaacccgt ccgacaggtc gatcaccccg gcaatccagc cgcccgcctc
     1621 aaggacctgc gtggcataga gcagcagcag cggtccttgc ccagcgagcc aggtgcggtg
     1681 gtccggcagc aggcctccgg tcttcaacgc gatctgtgcc gcacccaccg tcatcacccc
     1741 gggcagggtc cagccggcga tcggcatcgg ccgctcgatc gctccggtcg ccagcaagac
     1801 gttggtcccc gacacggaat gggcggcgcc atcgcgatcg gaccagaata cgtgaccgtc
     1861 cggctcgatc gcccagacgg aagcgttcca atgagcctcg acagcactgt ctcgaaagcg
     1921 ccggatcagc gccagggccg ccgcgtcctc ggtatccttc gcgccgcgcg cctcaagccc
     1981 ctgccagatg cggccgccgg gtgccgcgtt ctcatccagc aacaccaccg acaggccgtg
     2041 tttcaccgct tcggtcgcgg cacccagacc cgccgggccg gcgccaacga taaccaggtc
     2101 ggggatcacc cgatgaattt caccagcctg ccagacggct cgcccagaac ctcgtcgtca
     2161 cgcatgacga cctggccgcg cacgatcgtg gcaatgggcc agccggtcac ctgcatgccg
     2221 gcgaaggggg tccagccgca gggcgcggcg atccaggatt cctcgatccg ccgctgccgc
     2281 ttcaggtcca ccagcgtgaa gtcggcatca tatccggcgg ccaaacggcc cttgccgacc
     2341 acgccataaa cccgagcggg accggcacac atgagatcga ccatgcgggt gagaggcagg
     2401 cggccggcgt tcacgtggtc cagcatgatc ggcaccagcg tctgcacacc agtcaggccg
     2461 gcgggacagt ccggccacgg cagcagcttc ttgtcccggg gatgcggcgc gtggtcggac
     2521 ccgatggtgt ccacggtgcc gttgttgatg gccttccagg ctgcctccat gtgctcgggg
     2581 ccgcggatcg gcgggttcat cacgccgaaa ccctccagcc ggtcgtagca atccggggca
     2641 acctgggtga ggtggttcac cagtacctca caggtcgcga tgtcgcggaa atccctcagg
     2701 tattccagct cctgcgcggt cgagacgtgc aggatatgcg ccgggcggcc ggtcttgcgg
     2761 gcaagcgcca tcagccgcct tgtgccgagg aacgcacatt cctcgtcgcg ccagaccatg
     2821 tggtaggaat acgggtcgcc cgacttgaac atcggcttgc gttcctgcag gcggtattca
     2881 tcctcgctgt ggaaggcgat gcggcggcgg ccggagcgca tggctttttc caggtgctcg
     2941 tcgtcctcga tcatcaggtc gcccgtggag ctgccggcga agatcttgac gccgcagacg
     3001 ccgcggccga gctccagttc aggcagcgat ggaatgttgg ttttggtgca gccgacatac
     3061 aggcccatgt cgcaccaggc gacggtttcg acatagtcct gcttcca
//
LOCUS       DASWQN010001284         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_653312, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001284 DASWQN010000000
VERSION     DASWQN010001284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653312"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VGC82_21710"
     CDS             complement(<1..224)
                     /locus_tag="VGC82_21710"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425948.1"
                     /translation="MSGSFALGFVTGLAAEARIASRFGYPVRVGGTPAGAGVAASELI
                     EQGATALVSFGLAGGLDPALRPGTVVVASIV"
     gene            complement(221..2146)
                     /gene="shc"
                     /locus_tag="VGC82_21715"
     CDS             complement(221..2146)
                     /gene="shc"
                     /locus_tag="VGC82_21715"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008491974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEY0425949.1"
                     /translation="MDTDAIISPVVDDATLLASVSRAVDALTRRQRPDGHFLFELEAD
                     ATIPAEYVLLEHYLARIDPDLEARIGVYLRSIQGRHGGWPLFHDGAFNISASVKAYFA
                     LKAIGDDPDAPHMRRAREAILAAGGAERTNVFTRAQLALFGLVPWRAVPVMPVEIMHL
                     PLWFPFHLSKVSYWSRTVIVPLLVLMALRPRARNPRGVQIAELFRTAPDQVTDWIRGP
                     YRSRWGRFFKGIDVLLRGAEPLFPKASRSRAIGKAVAFVTERLNGKDGLGAIYPAMAN
                     SVMMLDALGYAADHPDAAIAWQSVRNLLVVEKDRAYCQPCLSPVWDTSLAGHALAEAG
                     ISTQAACDWLIPLQVTDVVGDWAVRRPGLRPGGWAFQYNNPHYPDVDDTAVVAMLLHR
                     NGNPAYAEAVERAREWVIGMQSSDGGWGAFEPENNHHYLNHIPFADHGALLDPPTADV
                     SARCVSFLAQIGMTHEDPVMRRALAYLRREQEPDGSWFGRWGTNYIYGTWSVLCALNA
                     ANVPHSDPAMLRAADWLVSVQRDDGGWGEDEESYADAPHGRYTESTPSQTAWAVLGLM
                     AAGAIEHPATARGIAYLTRTQHSDGEWAEALYTAVGFPRVFYLRYHGYKLYFPLLALA
                     RYRNLIRGNARRVEVGF"
     gene            complement(2351..>2500)
                     /locus_tag="VGC82_21720"
     CDS             complement(2351..>2500)
                     /locus_tag="VGC82_21720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0425950.1"
                     /translation="EQDARRPKTRTALASNLALAGDWTDTGLPATIEGAIRSGRTAAN
                     VVLSL"
BASE COUNT          482 a          849 c          770 g          399 t
ORIGIN      
        1 acgattgagg cgaccacgac agtccctggc cgcaacgccg gatccagtcc accagccagc
       61 ccaaaactga ccagggcggt ggcgccctgc tcgatcaact cggacgccgc cactccagcg
      121 cctgccggcg tgccgccgac ccggacagga taaccgaagc ggctggcgat ccgcgcctcc
      181 gccgccagac cggtgacgaa cccaagtgcg aacgatccgc tcagaagccg acctcgaccc
      241 ggcgggcgtt gccgcggatc aggttgcggt accgggcaag ggcaagcagc ggaaaataca
      301 gtttatagcc atggtagcgc aggtagaaga cacgcggaaa accaaccgcg gtatagagcg
      361 cctcggccca ttcgccatcg gaatgctggg tccgcgtcaa gtaggcgatg ccgcgcgctg
      421 tggcgggatg ttcgatggcg ccggccgcca tcagtcccaa aaccgcccag gcggtctggc
      481 tgggcgtgct ttccgtgtag cggccatgcg gcgcgtcggc gtagctttcc tcatcctcgc
      541 cccagccgcc atcgtcacgc tgcacggaaa ccagccagtc cgccgcccgc agcatcgccg
      601 gatcgctatg tgggacattg gccgcgttca gagcgcagag caccgaccat gtgccgtaga
      661 tatagttcgt tccccaccgc ccgaaccagc ttccatccgg ctcctgctca cgccgcagat
      721 aggccagcgc gcgacgcatc accgggtctt catgggtcat gccgatctgc gccagaaacg
      781 agacgcagcg cgcactgaca tcggctgtgg gagggtccag cagtgcgcca tgatcggcga
      841 acgggatgtg gttcagataa tgatggttgt tttccggctc gaacgcaccc caaccgccat
      901 cggatgactg cataccgatc acccactccc tcgcccgctc gacggcttcg gcataggccg
      961 gattgccatt cctgtgcagc aacatcgcca cgaccgccgt gtcgtccacg tccgggtaat
     1021 gcggattgtt atactggaac gcccagccgc ccggacgcag gcccggacgc cgcaccgccc
     1081 aatcgccgac gacatcggtg acctgtaaag gtatcagcca gtcgcaagcc gcctgcgtcg
     1141 aaatgccggc ctcggccagg gcatggcccg cgagactggt gtcccagacc ggagacaggc
     1201 agggctggca ataggctcgg tccttctcga ccaccaatag attgcgcacc gattgccacg
     1261 cgatggcggc gtcgggatgg tccgcggcgt agcccagggc gtcgagcatc atcacgctgt
     1321 tggccatcgc cgggtagatc gcgcccaacc cgtccttgcc gttgagccgt tcggtcacga
     1381 aggcgaccgc cttgccgatc gcacgggacc gggatgcctt tgggaacaac ggttcagccc
     1441 cgcgcagcaa gacgtcgata cctttgaaga accgccccca gcgagatcga tacgggccgc
     1501 gaatccagtc ggtcacctgg tcaggagccg tacggaacaa ttccgctatc tgcacgccgc
     1561 gcggattgcg cgcccggggg cgtagcgcca tcagcaccag cagcggcacg atcaccgttc
     1621 gcgaccagta cgataccttc gacaggtgga acggaaacca tagcggaagg tgcatgattt
     1681 cgaccggcat gaccggcacc gcacgccagg gcaccaaacc gaaaagcgcc aactgcgccc
     1741 gggtaaacac attggtccgt tccgcgccac cggccgccag gatcgcctca cgcgctcggc
     1801 gcatatgtgg cgcatccggg tcgtcgccga tcgcctttag cgcgaaatac gcctttacgc
     1861 tggcggagat gttgaacgcg ccatcgtgga acaacggcca gccgccatgc cgaccctgaa
     1921 tggaacggag gtaaacgccg atcctcgctt ccaggtcggg atcgatgcgc gccaggtaat
     1981 gctccagcag gacatactcg gccggaatcg tcgcatccgc ttccagttcg aacaggaaat
     2041 gcccatccgg acgttgccgc cgcgtcagcg catcgaccgc ccgggagacc gaagccagca
     2101 acgtcgcgtc gtcgacgacc ggcgagatga tcgcgtcggt gtccatccta tcccttaacg
     2161 gcccggctac gaattgctgc ttcggttcga gctcgcgccg atagatcggc cgtggtggac
     2221 gaaggcccac cagccacggc ttcgggtgct aaatctggca caggcgtgga tggtgggcct
     2281 tcgcccacca tgacggaaat ggtcggatcg tccgcaagtc ggggcagcac ttcataacca
     2341 ggccctaaag tcagagtgaa agcacgacat tggccgcggt tcggcccgac cttatggcgc
     2401 cctcgattgt ggccggcaac ccggtgtccg tccaatcgcc cgcgagtgcg aggttgctgg
     2461 cgagagccgt ccgggttttc gggcgccttg cgtcctgctc
//
LOCUS       DASWQN010001285         4066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1841525, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001285 DASWQN010000000
VERSION     DASWQN010001285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841525"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VGC82_21725"
     CDS             complement(<1..797)
                     /locus_tag="VGC82_21725"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEY0425951.1"
                     /translation="MRQNAAGKRGQTLVIFALIFVTLVGFAGLALDATHLYLAQHTAQ
                     KAADAAALAAGKRLAGATQQSPMANSNDLAVIAAHDFAAADGFITTRTTACDTTTTVG
                     SITRFTATWTDAASCSSTTFTNQVSVAVPPFTLTPHCQITPYNCMQVTIQSVVTNYVM
                     GTAGIPTSRVQGSATVYAQPTGTTFDYPYPVAVYLYQPAVATIAACPAGQQCFDRTKV
                     PARTLLSCSTAGSNCPTYWARPEASSLIVGVDGTTLNPPQDVVALES"
     gene            complement(813..1310)
                     /locus_tag="VGC82_21730"
     CDS             complement(813..1310)
                     /locus_tag="VGC82_21730"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY0425952.1"
                     /translation="MVEFALLVPIIFMTLFLSIDFARLVYTYAAVSWAARQAARVVSM
                     QPQSSSDCLAYQTAESTAQGFVLRADGNSIVGNADPNSTGTPNADPTPPAGVGKIYIW
                     PAVATAAPADAAANCNGSARAVSASVQDVAVQVKYTYQPLMPMISQMIPPITLTTISV
                     VHVEY"
     gene            complement(1519..1917)
                     /locus_tag="VGC82_21735"
     CDS             complement(1519..1917)
                     /locus_tag="VGC82_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425953.1"
                     /translation="MSGVAASAKLDSSSNQNGQQGQQNQSQNDKKTAPKPATKPADKP
                     AVAATKPAATKTKPATTATSPAATTAGQCGFDGEQTGEHEDAACNDTNGQAGANESKS
                     EANETADLEKNDNQQGNNQQGDNQQGDNQD"
     gene            complement(2100..2597)
                     /locus_tag="VGC82_21740"
     CDS             complement(2100..2597)
                     /locus_tag="VGC82_21740"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY0425954.1"
                     /translation="MEMALISPLLFFILLGITDIGRAYYQYTVMTDAVREGARYVAAQ
                     WSNSAGGAAATNSNAAAAPFNSVQGRIQYVGNTAGMTFANDASHIAITYYDGTSAALT
                     QCAHWDAATNAIVQDNSYPVTQPRTGDIVKIHLVYTFRPIAPILSQVTGAALNLTADA
                     QVRIE"
     gene            complement(2633..3757)
                     /locus_tag="VGC82_21745"
     CDS             complement(2633..3757)
                     /locus_tag="VGC82_21745"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEY0425955.1"
                     /translation="MKRRKFRQQKGQAIVLVALMVVALSGMLALTVDVGNSYTQRRLL
                     QTAADAAAMAGTHLGMEQITNAGGTQDYSAQRTTAIKFANFNGATGSDTITVVWVDNT
                     GVAFANNGSNLPIIPAGKVVQGMKVTIAGNRNTFLFRALGIQSVGVSVSAMAQFGTAN
                     AIVGASPLLMNSDAGSGSHSLYTPFLVTTDNGGPACCSSGQNDSLGRAIPSTFTSSPP
                     GQQNFHILHDPPTSSVALATRSSAANGISGTLSIGPNYYIETAGQPANDNFALGLMDR
                     IQRSIANPLFAGDRPQAGNFSPYNPRVFMVAVNNSASASSPTTIKEFLAFYIEFVIYD
                     ASHKVIIGGYWVNSSGVPGSGGLGVPTNGTGNPMVFRLTQ"
     gene            complement(3754..>4066)
                     /locus_tag="VGC82_21750"
     CDS             complement(3754..>4066)
                     /locus_tag="VGC82_21750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425956.1"
                     /translation="QGRIRLADGGLAIPNNAAHITIQFYDMTASPVALCAHFDYATNA
                     ISWDAPYTAPNSGSGQKCPKEGDGVKITAVSDYTPITPLMAQIMGNPFHLTATAETRI
                     E"
BASE COUNT          651 a         1220 c         1335 g          860 t
ORIGIN      
        1 ttcgactcca gtgcgacgac atcctgcggc gggttgagcg tggtcccgtc gacccccacg
       61 atcaggctgg aggcctccgg ccgagcccag taggtcgggc agttgcttcc tgcggtgctg
      121 cagctcagca gggtgcgcgc cggaaccttg gtccggtcaa agcattgctg tcccgccggg
      181 caggcagcga tcgtcgcgac ggccggctgg tagaggtaaa ccgcgaccgg gtacggatag
      241 tcgaaggtgg ttcccgttgg ttgggcatag acggtggcgc tcccctgcac ccggctggtg
      301 gggatcccag cagtacccat gacgtagttc gtcaccaccg actggatcgt cacctgcatg
      361 cagttgtagg gggttatctg gcagtgcggc gtcagcgtaa agggaggaac cgcgacactc
      421 acctggttcg taaaggtggt gctggaacag gaagcagcgt ccgtccaggt tgctgtgaac
      481 ctcgttatgc tgccaaccgt cgtagtcgta tcgcaggccg tggtacgcgt cgtgatgaag
      541 ccgtcggccg ccgcgaagtc atgggcggcg atgaccgcga ggtcgttgga gtttgccatc
      601 ggcgactgtt gcgtcgcccc cgccaaccgc ttgccggcgg cgagcgccgc cgcgtcggcc
      661 gccttctgag ccgtgtgctg cgcgaggtag aggtgagtgg cgtccaacgc aaggccggcg
      721 aacccgacca gcgtgacaaa aatgagggcg aagatgacca gggtctggcc acgtttgccc
      781 gccgcgtttt gccgcatcca ggtaatcacg ttctagtact ccacgtggac cacgctgatc
      841 gtggtgaggg tgatcggggg aatcatctgg ctgatcattg gcatcaaggg ctggtaggtg
      901 tacttaacct gtacggccac gtcctgcacg ctcgccgaga ccgcacgggc ggagccgttg
      961 cagttagcgg ccgcatcggc gggcgctgct gttgcgacgg cgggccagat gtagatcttg
     1021 cccacgccgg ccggcggtgt ggggtccgca ttcggcgtcc ccgtgctgtt cgggtcggcg
     1081 tttccgacga tcgagttgcc atcggcgcgg agtacgaacc cttgtgccgt cgactcagcc
     1141 gtctggtagg cgaggcagtc gctgcttgat tggggctgca tcgataccac gcgggcggcc
     1201 tgccgagctg cccaggagac ggctgcatag gtgtagacga gccgggcaaa gtcaatcgac
     1261 aggaacagcg tcataaagat gatcggcacc agcagggcga attcgaccat cgattgcgcg
     1321 ctgcttgccc gacgacggcg ggcgcgggtc gtgttcagca ccttcgctac ggtacgtcca
     1381 cttggatacg aagaagattg caacttttgg ttccaggtgg gcctgcaggc ttgtcaccaa
     1441 acggtcaccg ttccgttgcg gtagcagttg ctacaaatgg aaagaggccg ccgaagcggc
     1501 ctctttctgc tgcggggctt agtcctggtt gtcgccctgc tggttgtcgc cctgctggtt
     1561 attgccctgc tggttgtcgt tcttctcaag gtcggccgtc tcgttggcct cgcttttcga
     1621 ctcgttcgcg ccggcctggc cgttggtgtc gttgcatgcg gcgtcctcgt gctcgccagt
     1681 ctgctcgcca tcgaaaccac actggcccgc cgttgtagcc gcggggctgg tcgcggtcgt
     1741 cgcaggcttg gtcttcgtcg cggcgggctt ggtcgccgcc acggccggct tgtcggcagg
     1801 cttggtggcc ggcttcggcg ccgtcttctt gtcgttctgc gactggttct gctggccctg
     1861 ctggccgttc tggttgctcg agctatcgag tttggcactg gccgcaacgc cgctcaccag
     1921 gagcagacca ccggcgataa gtgcgatgac gatcctacgc atatgagtca gtctccttcg
     1981 gacaaagcgg taacttcgtc cacttactta acggctcgac cataccggct attcgtctcg
     2041 atgctgcttg cgactggagc ctgtagcgcc gccgtaccgt gctcgttacg aggccgtaac
     2101 tactcgatcc ggacctgggc atccgcggtc aggttcaagg cggcccctgt cacctggctc
     2161 aggatgggcg cgatcggccg gaaggtgtag acgaggtgaa tcttgacgat gtcgccggtc
     2221 cgcggctggg tgaccggata gctgttgtcc tggacgatcg cgttggtcgc tgcgtcccag
     2281 tgggcgcact gggtcaaggc tgcgctcgtc ccgtcgtaat acgtgatcgc gatatgagat
     2341 gcatcgttag cgaaggtcat ccccgccgtg tttccgacgt attggattcg cccctgcaca
     2401 ctgttgaacg gggcggcggc agcatttgag ttagtggcgg cggcgccccc agcactgttt
     2461 gaccattggg ccgcaacgta gcgggcacct tcgcggaccg catccgtcat gaccgtgtac
     2521 tggtaatacg cacggccaat gtcggtgatt ccgagcagga tgaagaaaag cagcggcgat
     2581 atcagcgcca tctccaccag cgactggccc cggctcctcc gacgtaggcc cgtcattgag
     2641 tcagccggaa taccatcggg ttcccggtgc cgttggttgg gacgccgagg ccgccgctgc
     2701 ccgggacgcc actcgagttg acccagtagc cgccgatgat gaccttgtgg ctggcgtcgt
     2761 agatgacgaa ctcgatgtaa aaggccagga actccttgat cgtggtcggg ctgctcgcgc
     2821 tggcactgtt gttgaccgcg accatgaaga cacgagggtt gtacggcgag aagttaccgg
     2881 cctgcggcct gtcccccgcg aacagcgggt tggcaatcga ccgctggatc cggtccatca
     2941 gcccgagcgc aaagttatcg ttggcaggct ggcccgccgt ctcgatgtaa tagtttggcc
     3001 cgatgctgag agtgccgctg atcccgttgg cagcgctgga ccgggtggcg agcgccaccg
     3061 agctcgtcgg gggatcatgc aggatgtgga agttttgctg gccgggcggg ctgctcgtaa
     3121 aggtgctggg gatggcgcgt ccaaggctgt cgttctgccc cgaggagcag catgccggtc
     3181 caccgttgtc ggttgtcacg aggaacggcg tataaaggct gtgcgagcct gatccggcgt
     3241 cactgttcat cagcaacggc gaggcgccta cgatcgcatt tgcggtcccg aactgcgcca
     3301 tggcgcttac cgaaaccccg actgactgga ttcccagggc gcgaaacagg aaagtattcc
     3361 ggttccccgc aatcgtcacc ttcatgccct ggacgacctt cccggccggg attatcggca
     3421 ggttgctccc gttgtttgcg aaagccacgc cggtgttatc gacccagaca acggtgatag
     3481 tgtcggaccc agttgccccg ttgaagttcg cgaacttgat cgccgttgtg cgttgtgcgc
     3541 tatagtcctg cgttcccccg gcattcgtga tctgctccat gccgaggtgc gtgccggcca
     3601 tggccgctgc atcggccgct gtttgaagca gccgcctttg cgtgtagcta tttccgacgt
     3661 caaccgtcag cgccagcatg ccgctgagcg cgaccaccat cagcgccacc agcacgatcg
     3721 cctgcccttt ttgctgccgg aacttcctgc gtttcattcg atcctcgttt ccgcggtggc
     3781 ggtcaaatgg aatgggttgc ccatgatctg ggccatcaac ggcgtgattg gcgtgtagtc
     3841 ggaaacggcg gtgatcttca cgccgtcacc ttctttcgga catttctgtc cgcttccgct
     3901 gttcggcgcg gtgtagggcg cgtcccagct gatcgcattc gtggcgtaat cgaaatgcgc
     3961 gcagagcgcg accgggcttg ctgtcatgtc atagaactga atggtgatgt gcgcggcatt
     4021 gttcgggatt gccagtcccc catccgcaag gcgaatccgg ccctgt
//
LOCUS       DASWQN010001286         5971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1841533, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001286 DASWQN010000000
VERSION     DASWQN010001286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841533"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VGC82_21755"
     CDS             <1..766
                     /locus_tag="VGC82_21755"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HEY0425957.1"
                     /translation="AAGMAPLTSMFLYDTNDHLRFDDWRAAVHDSSGLQIHTGHDEAI
                     WRPLTNPRNLQISAFEDIGPRGFGLLQRKRTFFDYHDLESHYEKRPSLWIEPIGDWGH
                     GVVELVEIPSDREVNDNMVAFWRPHDPMRAKGEYLLNYRLHWCWAPPNDARLAQVMQT
                     RCGLSFDQKHRQFIIDFVGDSLKALKPDSPPALDAASDKGKIVSAFTQPNPDVNGWRV
                     SIELDTQDNKLVELHARLMQGDQPLTETWIYRWTEG"
     gene            763..2895
                     /gene="mdoH"
                     /locus_tag="VGC82_21760"
     CDS             763..2895
                     /gene="mdoH"
                     /locus_tag="VGC82_21760"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEY0425958.1"
                     /translation="MTFRPLPDESPLAMPTQSLRDAPRRTARFASSPRAMGLRRLVVV
                     GGAVVMTAFGAREMYRVLAVNGATPLAIFMLALFVALFAWIALSFTSAIAGFVSVITG
                     GGRRLVAPGGLPVSRTALLMPTYNESPHRIMAGLEAIDEELRALRASDLFDLFILSDT
                     TQPETWIEEEKAFLDLRARTGGTTRIFYRHRAKNTARKAGNIAEWVGRFGGAYPQFLI
                     LDADSVMRGDILVSLVRAMEAHPDVGLIQTLPIITGGTTLFARMQQFAGRVYGPVIAH
                     GIAWWHGSEGNYWGHNALIRTVAFAEQAGLPELPGRKPFGGHIMSHDFIEAALMRRGG
                     WAIHMMPGLEGSYEESPPSLMDLAIRDRRWCQGNLQHIAILPARGLNWVSRMHLLTGI
                     GSYITAPLWLLFLLSGILIALQARFIPPDYFPDAGKALFPQWPVIDPVRAMWVFIGTM
                     GLLLVPKLLGMITVLVRRDLRRGCGGFSGLVVSLLLETLIAGLLAPVVMLTQTIDVVA
                     ILLGRDSGWNAQRRDDGAIPLRDIARLYRRHTILGLLLGLGGWAVSPYLALWMLPVVL
                     GLALAVPLASLTGRRSLGVALRRVGLLRIPEESNPPAVLQRASSLYRQAADAPMDSLS
                     ALLHDPELLAAHRRMLPPARRPGIDPLNIPLVIGAARLSEAAILDTVWPTLSAAEKTA
                     VLSDGPAIDRLVMLSDPRTARNPASVAR"
     gene            3192..3761
                     /locus_tag="VGC82_21765"
     CDS             3192..3761
                     /locus_tag="VGC82_21765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0425959.1"
                     /translation="MSDTSASTNPRSKPFLSDVQTLRDRARRNLSEGVVTSTYGGDVK
                     KTIEILQSVLATEIVCLLRYTMHSVTATGISSEAIKAEFAEHAKEEQEHMMAVAERID
                     QLGGTPNFNPEGLATRSASQYAEGDNLVDMIRENLIAERIAVDHYRELIRYFADDDPS
                     TRTMLEGILSVEEEHATDMQDLLEAHQGT"
     gene            3758..4645
                     /locus_tag="VGC82_21770"
     CDS             3758..4645
                     /locus_tag="VGC82_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425960.1"
                     /translation="MICALIRSCNAAPATAAACAALLLADQALAQPAPTPTGANANRT
                     VNLNYVYAAQLGFGGYSLAGLSADVYTLPLADTLQNLPYEGWALKLLLPVQAGIYSFR
                     ATDVGGQRIAIDQQSVSVVPGAELQIPLGERIVMKPFAQVGEAHAFGTGAGNADSWIF
                     LAGVRALTQWHAGDYTISLGNAVVYAGDQTIGPGFAEHYVSLQLGAEVRRPLGFQVGD
                     IAPDIGVYVADYYYPAPLQFSRFLKPTLRVANQNEIGLSIGSATPFQMLWLANPRIGV
                     GFVFGGGLNVWHVNFGFQF"
     gene            complement(4774..5127)
                     /locus_tag="VGC82_21775"
     CDS             complement(4774..5127)
                     /locus_tag="VGC82_21775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425961.1"
                     /translation="MAQCVFRLLAWMQVGRREQGAVFVPNFNRLTGRQYGFLAGAWKR
                     QACLHLRHRHSAVQVFHRKLALVSALKDVKLVDRTAHQCGPKTARHPQESLVYLDVSY
                     VGTPDDQASAGLAPN"
     gene            5565..5867
                     /locus_tag="VGC82_21780"
     CDS             5565..5867
                     /locus_tag="VGC82_21780"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY0425962.1"
                     /translation="MSGIAPRPFHRLPARPRAREVLDLLAQGLSDTATAAKSDVTSDT
                     VCNHVAAIYKKLGVNRCGAAVAWARERGFGAEPKILTKLINLGATNGRSNFVQGTR"
BASE COUNT         1055 a         1909 c         1856 g         1151 t
ORIGIN      
        1 cgccgccggc atggcgccgc tgaccagcat gttcctgtac gacaccaacg accacttgcg
       61 cttcgacgat tggcgggcgg cggtgcatga ctcgagcggg ttgcagatcc acaccggaca
      121 tgacgaggcc atctggcgcc cgttgaccaa tccccggaac ctgcagatca gcgctttcga
      181 agacatcggt ccccggggct tcggcctgct gcagcgcaag cgcacctttt tcgattatca
      241 cgacctggaa agccattacg aaaagcggcc gtcgctctgg atcgagccga tcggcgactg
      301 gggccacggc gtcgtcgagc ttgtggaaat tcccagcgac cgcgaggtca acgacaacat
      361 ggtcgcgttc tggcgcccgc acgatccgat gcgagcgaag ggcgaatact tgctgaacta
      421 ccggctgcac tggtgctggg caccacccaa cgacgccagg ctggctcagg tgatgcagac
      481 ccgctgcgga ctgtccttcg atcagaagca ccgccagttc atcatcgact tcgtcggcga
      541 ctcgctgaag gcgctaaagc cggacagccc acccgcgctc gatgccgcct cggacaaagg
      601 caaaatcgtc agtgccttca cgcaaccgaa ccccgacgtc aacggttggc gcgtgagcat
      661 cgaactcgac acgcaggaca acaagcttgt ggaactgcac gcgcgcctga tgcagggcga
      721 ccagccgctg acggaaacct ggatctaccg atggaccgaa gggtgacctt tcgtcccctg
      781 ccggacgagt cgccgctggc gatgccgacg cagtcgttgc gtgatgcgcc aaggcggacc
      841 gcccgcttcg cctcctcgcc tcgcgccatg ggtctgcgcc gcctggtcgt tgtcggtggc
      901 gccgtcgtca tgaccgcgtt cggcgccagg gaaatgtatc gcgtgctggc ggtgaacgga
      961 gcgacgccgc tggccatctt catgctggcg ttgttcgtcg cgctgttcgc ctggattgcc
     1021 ttgtcgttca ccagcgcgat cgccgggttc gtttccgtca tcaccggcgg aggccgccga
     1081 ctggtggcgc cggggggctt gcccgtcagc cgcaccgcgt tgctgatgcc gacctacaat
     1141 gaatcccccc accgcatcat ggccgggctg gaggcgatcg acgaagaact ccgcgccctg
     1201 cgagccagcg accttttcga cctctttatt ctgagcgaca ccacgcagcc ggaaacctgg
     1261 atcgaggagg aaaaggcgtt cctcgatctc cgtgcgcgaa ccggcggtac aacccgcatc
     1321 ttctaccgcc accgggcgaa gaacaccgca cgcaaggccg gcaatatcgc cgaatgggtc
     1381 ggccggttcg gcggcgccta tccccagttc cttatcctgg atgccgacag cgtcatgcgc
     1441 ggcgacattt tagtgtccct ggttcgcgcg atggaggcgc atccggacgt cggcctgatc
     1501 cagaccctgc cgatcatcac cggcggcacc accttgttcg ccaggatgca gcaatttgcc
     1561 ggccgggtgt acggtccggt catcgcccat ggcatcgcct ggtggcatgg ctccgagggc
     1621 aactactggg gccacaacgc gctgatccgc actgtcgcct tcgctgaaca ggcagggctg
     1681 cccgagttgc ccggccgcaa acccttcggc ggtcacatca tgagccacga cttcatcgag
     1741 gctgccctga tgcggcgcgg tggttgggcc atccacatga tgccgggtct ggaagggagc
     1801 tacgaggaga gtccgccttc gctgatggac ctggcgatcc gcgaccgtcg ctggtgccag
     1861 ggcaacctgc agcacatcgc cattctgccg gctcggggcc tgaactgggt cagccggatg
     1921 catctgctga ccggtatcgg atcctacatc acggccccgt tatggctgct gttcctgctc
     1981 agcggcatcc tgatcgccct gcaggcgcgg ttcatcccgc ccgattactt ccccgatgcc
     2041 ggcaaggcgt tgttcccgca atggccggtg atcgatccag tccgagcgat gtgggtgttc
     2101 atcggcacaa tgggattgct gctggtgccg aagctgctgg gaatgatcac ggttttggtt
     2161 cggcgcgatc tgcggcgggg ctgtggcggc ttttccggac tggtcgtcag cctgttgctg
     2221 gaaacgctga tcgccgggct gctggctccg gtggtgatgc tgacccagac catcgatgtc
     2281 gtcgcgatcc tgctggggcg ggattccgga tggaatgcgc aacgccgcga cgatggcgcc
     2341 atcccgttgc gcgacatcgc ccgactctac cggcgccaca caatccttgg cctgctgctc
     2401 ggtctcggcg gctgggcggt ttcgccatac ctggcattgt ggatgctgcc ggtcgtgctt
     2461 ggtctcgcgc tggctgtccc gcttgcctcg ctgaccggac ggcgctccct gggtgtggca
     2521 ctgcgccgtg tggggctgct tcgcataccg gaggaaagca atcctcccgc ggtgctgcaa
     2581 cgcgcctcat cgctgtaccg ccaggcagcc gatgccccca tggacagtct gtccgccctg
     2641 ttgcacgacc cggagctgct ggctgcgcat cggcggatgt tgccgcccgc acgtcggccc
     2701 gggatcgatc cgctgaacat tccgttggtc attggggcag cgcgactcag cgaggcggcc
     2761 attctggaca ccgtttggcc gacgctgagt gcggcggaga agaccgcggt gctgtcggac
     2821 ggacctgcca tcgaccggct ggtcatgttg tcggatcccc ggacagcccg aaatccggcc
     2881 agcgtcgcca ggtgatattc gcgagttgat attcgggccc gaaagtcccg cccaggcact
     2941 gaccgcgtgg acccttgagg aaaagccggc cggactgcct acccggacgg accgccgccc
     3001 cgccggactg cctggcgcgg acggactgcc tgttggtccg gctatcggat tagtgtgcca
     3061 agactagggg actatcttga cctgctctcc actgccccta ctacacgata ccaggcaacg
     3121 ccccacttgc acctcgcaac gccttcgatt tcgtgcaacc gactcaggta accgacgccc
     3181 aggagagcat catgagtgac acatccgcga gtacgaaccc acgctcgaag ccctttcttt
     3241 ccgatgtcca gacattgcgg gaccgggccc gccggaacct gtcggaaggg gttgttacct
     3301 cgacctacgg cggcgacgtg aaaaagacca tcgaaatcct gcagtccgtc ctggccaccg
     3361 agatcgtctg cctcctgcgc tacacgatgc actcggtcac cgcgaccggt atttccagcg
     3421 aagcgatcaa ggcggagttc gccgaacacg ccaaggaaga acaggaacac atgatggccg
     3481 ttgccgaacg catcgaccag ctcggcggca cccccaactt caaccccgaa ggcctcgcca
     3541 cccgttcggc ctcgcaatac gccgagggcg acaaccttgt ggacatgatt cgtgaaaacc
     3601 tgatcgcgga gcgcatcgcg gtcgatcatt accgggaatt gatccgctat ttcgccgacg
     3661 acgacccctc gacccggacg atgctggagg gcatcctgtc ggtcgaagaa gagcacgcaa
     3721 ccgacatgca ggatctgctg gaggctcacc agggtacgtg atttgcgcgc tgatcaggtc
     3781 ctgcaatgcg gcacccgcca ccgcggcggc gtgtgccgca ttgcttttgg ccgatcaggc
     3841 gcttgcccaa cctgccccta cccctaccgg cgccaacgcc aaccgcaccg tgaacctcaa
     3901 ttacgtgtat gccgcccagc ttggttttgg cggttattcg ctcgcgggcc tgagcgccga
     3961 tgtctataca ttgccgctgg ccgatacgtt gcagaacctg ccgtatgaag gctgggcgct
     4021 gaagctgttg ctgccggttc aggcgggtat ctacagcttc agggccaccg atgttggtgg
     4081 ccagcgcatc gccatcgacc agcaatcggt gtcggtcgtt ccgggcgctg aattgcagat
     4141 cccgctcggc gagcggatcg tgatgaaacc gttcgctcag gttggcgaag cgcatgcctt
     4201 cggcaccggc gccggaaacg ccgattcgtg gatcttcctg gcgggggttc gtgcgctgac
     4261 gcaatggcac gccggcgatt atacgatttc ccttggtaat gctgttgtct atgcgggtga
     4321 ccagacgatt ggaccaggtt tcgccgaaca ctacgtttcg ctgcaacttg gcgccgaagt
     4381 acgacgtccg ctgggttttc aggttggcga catcgctccc gatatcggtg tttatgtcgc
     4441 cgactattac tatccggcgc cactgcagtt ctcgcggttc ctgaagccga cgctgagggt
     4501 ggccaaccag aacgagatcg gtctctcgat cgggtcggcc acgccattcc agatgctttg
     4561 gcttgccaac ccgcgtatcg gcgtaggttt tgttttcggc ggtgggctga atgtctggca
     4621 cgtcaacttc gggttccagt tctgaccggc ggtcgagacg gaacgtacct catggtttcc
     4681 cgggaaatgg tcgcttcaaa tgcggcttcg gattccatcg cggtcggcgg aggcccacca
     4741 tgatagtgac cgaacggttc gccgggttcg aagttaattt ggcgccaaac cagcgcttgc
     4801 ctgatcatct ggcgtgccga catacgacac gtcaaggtaa accaagcttt cctggggatg
     4861 acgggctgtc ttcgggccgc attggtgggc tgttctgtcc acgagcttga catccttcag
     4921 ggcgctgaca agcgcgagct tgcggtggaa cacctgcacg gcggaatggc ggtgccgcag
     4981 atgcaggcat gcctgccgct tccaggcgcc ggcgagaaaa ccgtactgcc tgccggtcag
     5041 acgattgaaa ttgggcacga acaccgcacc ttgttcacgt ctgccgacct gcatccatgc
     5101 aagcaggcgg aagacgcact gcgccaaagc gaggagcgat cttccaaggc gctccaaatg
     5161 gcaccgggac cggtggcgac cgtcgcgctg gaaggattcc gcatcctcta cgtgaaccag
     5221 atttttgcct cggcgacggg ctggcggctt gaagaagccg tgcgccgagg cgaactggac
     5281 tggcgttgcg gcgcaatgcc gcagcgcggg cgcaactgtg gcggcaggtc cagcagaccg
     5341 gacacatcag cagcatcgac atcgaggtcc ggactggatt tgtgaccacc tgctgtcggc
     5401 ggaaaccgtc gcggtccagc aggagatgtg catcttaagt gtcatgcttg tcatcaccga
     5461 gcgtcggaaa acggggatgc ggttgctgtc cgccattgag tcggtcatgc aggatacgtc
     5521 ctggtttggc caaaagatcg tcgagaaatc gatcggcatt acgggtgtcg gggatcgccc
     5581 ccaggccgtt ccacagattg ccagcccgac cccgcgcccg ggaggtgctc gacctgctgg
     5641 cgcagggtct ttccgacacc gccaccgccg cgaagtcgga tgttacatcc gataccgttt
     5701 gtaatcacgt cgccgcgatc tacaagaaac tcggcgtcaa tcgctgcggc gcggccgtcg
     5761 cttgggcgcg agagcgtggt ttcggagcag agccgaagat attgacaaag ctgataaatc
     5821 tcggcgccac aaacggccgc agtaacttcg tccagggtac ccgatagttt tcgtcttgac
     5881 ctgttcggtt ttgtcttcgc ggcgttcggc ttcccgtggt ggaacttatc gccacgcacc
     5941 gcgctgtgcc tgtgcgaagc agcaagcgtc c
//
LOCUS       DASWQN010001287         3006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_521012, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001287 DASWQN010000000
VERSION     DASWQN010001287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521012"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..949
                     /locus_tag="VGC82_21785"
     CDS             164..949
                     /locus_tag="VGC82_21785"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEY0425963.1"
                     /translation="MSLKRKVALVTGSTSGIGLGIARALAAAGADIMLNGFGDAAEIE
                     ALRQEIAASHGVKVNYSSADISQPEQIDAMVAETAATLGAVDILVNNAGVQFTANVED
                     FPNERWDQIIAINMSGVFHGMKAAIPGMKAKGWGRIINIASAHGLVASAQKVAYVAAK
                     HGVLGMTKVAAIELANAGVTVNAICPGWVMTPLVEKQLRDRATRDGTTIEYQTHAMVA
                     EKQPMTKFTTPEQVGGLAVFLCSDAAATITGAPLSIDGGWVAQ"
     gene            997..1596
                     /locus_tag="VGC82_21790"
     CDS             997..1596
                     /locus_tag="VGC82_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY0425964.1"
                     /translation="MQIHEAARFTAMMTKSADLKRQIATELADAVVKVVGACEASLRA
                     GGKLMFCGNGGSAADSQHLATEMLIRLRSGVERPSIAALALTLDPAMLTACGNDYGYD
                     RVFERPLRGLGRRGDVLFGITTSGRSANVIRALEAAREMGIVTVGLLGGTGEPAMSHC
                     DVALLVPDTETARIQECHIALGHGILELLEDRLLARPYQ"
     gene            1609..2583
                     /locus_tag="VGC82_21795"
     CDS             1609..2583
                     /locus_tag="VGC82_21795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0425965.1"
                     /translation="MKVLVTGCAGFIGYHVAEALLARGDTVIGADNLNDYYDVRLKQA
                     RLARLRQRAGFTFEHTDISDKAAVGALVTDHPNIAGIVHLAAQAGVRHSLVDPYAYVQ
                     ANVMGHLVMLEAARRLPELRHLVYASTSSVYGANTVLPFRETDRTDRPVSLYAATKRA
                     DELMSYAYGHLFDLRQTGLRFFTVYGPWGRPDMAYYSFAKAIVAGEPITLYDGGLLRR
                     DFTYIDDIVAGILGALDQPPEQEVPPVRVLNIGNHRGEEVRTLVALLEQALGQAAVVR
                     NAARPAADVAETFASVEAIGALTGFAPRTTLAEGIPRFVAWFKAWHGI"
BASE COUNT          573 a          800 c         1039 g          594 t
ORIGIN      
        1 ctatgacacg aggcaatgct cggtcatgat gcaacgcagt ataacagcat gatcgcgccg
       61 ggcggaacgt cctaagtgtg caatcatgcg acacactgac catttagccg gcagggtcag
      121 gctgataatg gtccaggatc ggatttgcgt ggaaggatta tgcatgagtc taaagcgaaa
      181 ggtcgcactg gtaaccggat cgaccagcgg tatcgggttg ggcatcgccc gcgctctggc
      241 ggcagcggga gcggatatca tgttgaacgg tttcggcgat gccgccgaga tcgaagcatt
      301 gcgtcaggag atcgcggcgt cgcacggggt gaaagtcaac tacagcagcg ccgacatcag
      361 ccagcctgaa cagatcgacg cgatggtggc ggaaacggca gcgacgctcg gggctgtgga
      421 catcctggtc aacaacgccg gcgtgcagtt taccgcgaac gtggaagatt tccccaacga
      481 gcggtgggac cagatcatcg cgatcaacat gtcgggcgtg tttcacggca tgaaagcggc
      541 aatcccgggg atgaaagcga agggctgggg gcggattatc aacatagcgt cggcgcatgg
      601 cctggtcgca agcgcgcaaa aagtcgccta cgtggccgcc aagcatgggg tgctggggat
      661 gaccaaggtg gcggcgatcg aactggccaa cgcgggcgtg acggtgaacg cgatctgccc
      721 cggctgggta atgacgccgt tggtggaaaa acagctccgg gatcgggcaa cgcgcgacgg
      781 aacgacgatc gagtaccaga cccacgcgat ggtcgccgag aagcagccga tgacgaagtt
      841 caccacgccg gaacaggtcg gtgggctggc ggtttttctt tgctccgacg ccgcggcgac
      901 aatcaccggg gcgccgcttt cgatcgatgg cgggtgggta gcgcagtaac ggacaggtga
      961 tgtagaactt tcaaggttca acgagacgga gtgcgcatgc agatccacga ggccgcccgc
     1021 ttcacggcga tgatgacgaa atcagcggat ctgaagcgcc agatcgcgac tgaactggcc
     1081 gatgcggtcg tcaaggtggt cggtgcgtgc gaggccagct tgcgggccgg cggcaagctg
     1141 atgttctgcg gcaatggcgg atccgcggcc gacagccagc atctggcgac cgagatgctg
     1201 atccggttgc ggtccggcgt ggagcgtcct tcaattgccg cgttggcatt gacgctggat
     1261 ccggcgatgt tgaccgcgtg cggcaacgat tatggctacg accgtgtgtt cgagcgtccg
     1321 ttgcgcggat tgggacggcg cggcgacgta ttgttcggca tcaccacctc tggccgatcg
     1381 gcgaatgtca tccgagcgct ggaggccgcg cgggagatgg ggatcgttac ggtcggactg
     1441 ctcggtggga cgggcgagcc ggcgatgagc cattgcgatg tggcgctgct ggtgccggat
     1501 accgagacgg cgcgcattca ggagtgccac atcgctctcg gtcacgggat cttggaactt
     1561 ttggaagatc ggctcctggc gcgcccgtac cagtgaggag cttgaccaat gaaggtgctt
     1621 gtcactggct gcgcggggtt cattggctat catgtggccg aggcattgct ggcccgtggc
     1681 gacacggtga tcggcgccga caacctgaat gactattatg acgtaaggct gaagcaggct
     1741 cggcttgccc gattgcggca acgggcagga tttaccttcg aacataccga catctcggac
     1801 aaggcggcgg tcggcgcgct ggtgaccgac catccgaaca tcgcggggat cgtgcatctc
     1861 gcggcccagg ccggggttcg acattcgctg gtggaccctt atgcctacgt gcaggcgaac
     1921 gtgatggggc acctcgtgat gctggaggcg gcgcggcggc tgccggagct gcggcatctg
     1981 gtttatgcca gcacatcatc cgtttacggc gcgaacacgg tgctgccatt tcgcgaaacc
     2041 gaccggaccg acaggcctgt gtcgttgtat gccgcgacca agcgcgcgga tgaactcatg
     2101 agctatgcct atgggcatct gtttgacctg cggcagacgg ggttgcggtt cttcaccgtc
     2161 tatggcccgt ggggacggcc ggacatggca tactacagct tcgcgaaggc gatcgtcgcc
     2221 ggggagccga tcaccttgta cgacggtggc ctgctgaggc gtgacttcac ctatatcgac
     2281 gatatcgtgg ccggaatact gggtgccttg gaccagcccc ccgaacagga ggttccgccg
     2341 gttcgggtgc tgaacatcgg caaccatcgt ggcgaggaag ttcgaacgct ggtcgcgctg
     2401 ttggagcagg cactggggca ggcagcggtg gtacgcaacg cggcgcggcc ggcggcggat
     2461 gtcgcggaaa ccttcgcttc ggtggaggcg atcggcgcgc taacgggatt tgcccctcga
     2521 accacgctgg cggagggaat cccgcgcttt gtcgcctggt tcaaggcgtg gcacgggatc
     2581 tgacttggcg gctgtccgcc ggctgcagga agaaaagccg gacccaatga aattcgcgaa
     2641 ccaggatttc atctgttttc cgtaggttgc cgttgtcacg cggcgagtcg gcaccggcgg
     2701 gcttcaattt tttgaaaata cctgcttgac ggtccggcgg cagaagccta aatccccgtt
     2761 caccgagacg gacagtttct cttgcgttgg ttcgtctctt agactaggtt ctccgatctg
     2821 caccaggatg gtgctcatca aagcagcttt agcctggtgg cccatagagt atgggtggcg
     2881 ggtcgatgtt tgcttttgtt ctttgaaatt tgaataggct aggaagggat acgttggcgg
     2941 cgtctgtgct gtttgctgtg tattaattta cgcggtggat gggattgggt ttggtttcgg
     3001 tttgga
//
LOCUS       DASWQN010001288         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2368531, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001288 DASWQN010000000
VERSION     DASWQN010001288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2368531"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1502
                     /gene="glmM"
                     /locus_tag="VGC82_21800"
     CDS             132..1502
                     /gene="glmM"
                     /locus_tag="VGC82_21800"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY0425966.1"
                     /translation="MTKTRRLFGTDGIRGRANTDPMTAGIALMLGQAAGLLFTRGEHR
                     HRVVIGKDTRLSGYMIEPALTAGFIGAGMDVTLLGPLPTPAVAMLTRSLRADLGVMIS
                     ASHNAYEDNGIKLFGPDGTKLSDEMELEIEALMERGFAERLADSHLLGRAARLEDAAG
                     RYVEQAKASFPRGLRLDGLRIVVDCANGAAYKVAPTVLWELGATVIPVGVTPDGFNIN
                     KDCGSTVPEFLCAKVLEHGAHLGIALDGDADRVIIADENGDVVDGDQILALIAQTWHQ
                     SGTLRGGGVVATVMSNLGLERHLARLGLRLHRTKVGDRYVAEHMRACGLNVGGEQSGH
                     LILSDFATTGDGLLAALQVLAVLVQQGRPASQVLQVFPRLPQRLKNVRFQGTSPLREE
                     RVQAAIREAEARLNGIGRVLIRESGTEPLVRVMAEAEDSAVVADVVDSLCEMIAGVAR
                     VRQGAV"
     gene            1502..2341
                     /gene="thiD"
                     /locus_tag="VGC82_21805"
     CDS             1502..2341
                     /gene="thiD"
                     /locus_tag="VGC82_21805"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY0425967.1"
                     /translation="MRGRVLVIAGSDSGGGAGIQADIKTITALAAFASTAITALTAQN
                     TLGVHDVMAVPPGFIRRQIEVVLTDIGADVIKIGMLGDVATTNSVADALADFAPNVPV
                     VLDPVMVAKGGHTLLAAEAVECLRRRLLPLAAVITPNLPEAEALTGMTIRTEADMRAA
                     AAALLAIGAPAVLLKGGHLDSDIVVDLLVTHGGVEAFAAPRIVTRHTHGTGCTTASAI
                     AAGLAQGLGLRDAVIRARLYVRAAISGAPGLGQGHGPLDHSVTVDLSFGRLHFPGRDG
                     LAG"
BASE COUNT          454 a          841 c          951 g          497 t
ORIGIN      
        1 cgcgtacacg atgtgcggga aaccgtgcag gccatgcgtg tctggtatac attgttcgcg
       61 gccgacacgg ttgcgcttcc tcaacacccc ggcagtggac tagaggcacc ggacgcggcg
      121 atggatcacg aatgaccaag acaagacgct tgttcggcac ggatggtatc cggggcaggg
      181 ccaacaccga cccgatgacg gccggcatcg ccctgatgct cggacaagcc gccggattgc
      241 tgttcacccg cggtgagcac cggcatcgag tcgtgatcgg caaggacaca aggctgtcgg
      301 gctacatgat cgagccggcc ctgaccgccg gcttcatcgg cgccggcatg gatgtgacgc
      361 tgctcggacc tttaccgact cctgcggtag ccatgctgac gcggtcgttg cgtgccgacc
      421 tgggcgtgat gatctcggcg tcgcacaacg cctatgagga caatgggatc aagctgttcg
      481 gccccgacgg taccaagctg tcggacgaga tggagcttga gatcgaggcg ctgatggaac
      541 gcggctttgc cgaacgcctt gcggactctc atctgcttgg cagggcggct cgcctggagg
      601 acgccgcggg acggtatgtc gagcaggcga aggcaagttt cccgcgcggc ctgcggttgg
      661 acgggctgcg aatcgtcgtg gattgcgcca acggcgccgc ctacaaggtt gcgccgaccg
      721 tgctatggga actcggtgcg accgtcatac ccgtcggcgt cacgcccgat ggtttcaata
      781 tcaacaaaga ctgcggttcc acggtgccgg aattcctctg cgccaaggtg ctggaacatg
      841 gcgctcacct gggcatcgcg ttggacggcg acgccgatcg agtgatcatc gccgatgaaa
      901 atggcgatgt ggtggacggc gaccagattc tggcgctgat cgcgcagacc tggcaccagt
      961 cgggaacgct gcgcggcggc ggcgtggtcg ccacggtgat gtccaacctc ggcctggaac
     1021 gccacctggc gcgcctcggc ttgcgcctgc accgcaccaa ggtcggcgac cgctacgtcg
     1081 cggagcacat gcgcgcttgc ggacttaatg tcggcggcga gcaatccggc catttgatcc
     1141 tgtccgattt tgctacgacc ggcgatggat tgctcgcggc gcttcaagtc ctggcggtgc
     1201 tggtgcagca gggccgccca gcctcgcaag tcttgcaagt gttcccgcgg ctgccgcagc
     1261 ggctgaagaa tgtgcggttc cagggtacct cgccgctgcg ggaggaacgc gtgcaggccg
     1321 cgatccgcga agcagaggcc cgcctgaacg gcatcgggcg tgtgctgatt cgtgaaagcg
     1381 gcacggaacc cctggtgcgg gtgatggccg aggcggagga cagcgccgtg gtcgccgatg
     1441 tggtggattc gctttgcgag atgatcgccg gcgtcgcccg cgttcggcag ggcgcggtat
     1501 gatgcgtggg cgagtcctgg tcatagccgg ttccgattcc ggtggcggcg ctggcattca
     1561 ggcggatatc aagacgatta cggcactggc ggccttcgcg tcgaccgcga tcaccgcgct
     1621 gacggcgcaa aacacgttgg gcgtccacga cgtgatggcg gtgccccccg ggtttattcg
     1681 ccgccagatc gaggttgtcc tgaccgacat cggtgccgat gtcatcaaga ttggcatgtt
     1741 gggcgatgtg gcgacgacga attccgtcgc ggatgcgctt gctgacttcg ccccgaacgt
     1801 gcctgtggtt cttgacccgg tcatggtggc caaaggcggc catacgctat tggccgccga
     1861 ggcggtcgag tgcctgcgcc ggcggctgct cccactcgcg gcggtgataa cgcccaatct
     1921 gccggaagcc gaggcgctga ccggcatgac gatccggacg gaggcggaca tgcgggccgc
     1981 cgccgccgca ctgttggcca tcggggcgcc tgccgtgctg ctgaagggag gccatctcga
     2041 ttcggacatc gtggtcgatc tgctggtgac ccatggcggt gtcgaagcct tcgccgcgcc
     2101 ccgcatcgtc accaggcaca cgcatgggac cggctgcacc accgcctcgg ccattgcggc
     2161 gggtctcgcc caaggccttg gcctgcgcga cgccgtcatc cgcgcgcgat tgtatgtgcg
     2221 ggcggcgatc agtggggctc ccggtctggg ccagggtcac gggccgctcg atcattcggt
     2281 gacggttgat ctaagttttg gccgcttgca cttccccggg cgggatggtt tggcggggta
     2341 gcgagaagcg tttcatcacg gattgcccgg atcaacgcac ggatctgagt cgatacccga
     2401 ttcaagctcg taacgcgttc cgggtggatg ccactgtggc ctggtgccag tgcggtccgg
     2461 tgccagtggg tccggtgcca gtgcggttcg acgcatggcg gcggttgctc cttgcgcgca
     2521 tcaggcgcta aggttctgcc cacgatcgga acagatcgac ggccaaaaaa taagcctgcg
     2581 cattcaggtg aaaccagggc gccggccgca ggggaaaaca aaatgtctcc gattttacgg
     2641 cgtaaactgc tgcaactttc gggaaccggc atggctgccg ccaccacggg cggcctggca
     2701 ggcattctgg cgtccggcca ggcgccggcc tatgcccagg gaa
//
LOCUS       DASWQN010001289         5119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1576272, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001289 DASWQN010000000
VERSION     DASWQN010001289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576272"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..765
                     /locus_tag="VGC82_21810"
     CDS             214..765
                     /locus_tag="VGC82_21810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY0425968.1"
                     /translation="MRLFLSVLLCLLVMACAPGRGLPDLPSIGPGAYRLGPGDVIRLI
                     TFGEEALTGEFRVSDSGMIALPLIGAVPAAGLSPDVLAVHVGEALVKANLLRAPSVSA
                     EVIAYRPIFVLGEVSKPGQYPYQPGMTVVTAVAVAGGFTYRAFNDYASVVRTRDGVAI
                     EGKAIRQSFVEPGDVISVFERRF"
     gene            795..1574
                     /locus_tag="VGC82_21815"
     CDS             795..1574
                     /locus_tag="VGC82_21815"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEY0425969.1"
                     /translation="MMPFAAVAESGGSASAPLHIIGGPNTGGCIGGAVSLPPDGPGFQ
                     TIHSNNSAFWGAPRTISHLEAFGREAAGAGLPTLLVEDISRIHGGPMPGGHVSHQIGL
                     DADVALDMRARPPLPASERDTIVLRSMVRPDGRDVDPGAWSPRVTTLLRLAAGLPEVD
                     RILVNPAIKQQLCREVAGDRSWLHLIRPWYGHAAHMHIRFRCPDGQPDCIQAPPPPAG
                     DGCDATLQWWFDQLDAPARPSSPSKVPPLPAACTAILAGGG"
     gene            complement(1984..2160)
                     /locus_tag="VGC82_21820"
     CDS             complement(1984..2160)
                     /locus_tag="VGC82_21820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425970.1"
                     /translation="MTDSKFEARVKATGLRPKPDDMSMLEAMVKDLDRAAAMLRGPRP
                     YADEPLNAFRLKPA"
     gene            complement(2171..3574)
                     /locus_tag="VGC82_21825"
     CDS             complement(2171..3574)
                     /locus_tag="VGC82_21825"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0425971.1"
                     /translation="MEPFLSIAEASGLIAAKKLSPVELTRMCLDRVKKLDPTLHSFLL
                     VTEDRALADAKAAEGRMMSATLKGPLDGIPIGHKDIYNTAGIRTTGHSRLLEHNVPNE
                     DSVVVGKWAAAGTVLMGKLATHEFAIGGPSFDLPWPPARNPWNPDYFTAGSSSGTGAA
                     VAAGLVLGGTGSDTGGSIRGPAALCGIAGIKPTYGLSSRRGVLPLSQTLDHTGPMAWT
                     AQDCALLLQGMAGHDPFDPASADRPVTNFTADLDKGVKGLRIGVIRHFHEVDHPVSPA
                     TRQGIEDALDIFGSRGAEICDVTLSPMMDYNAPGWLILTAEAYAVHEPWLKERFNDYG
                     ELLRDRLAFGALLQATDYVQALRRRRVLCLELRDAMADLDILITASAPGEAPKIHEVP
                     KWAMLEKPNFTMPFNLTGLPALSICTGYGAEGLPLSMQIVGKPFAEATVFRVGHAYET
                     ATTWRSRRPPMSIALAS"
     gene            complement(3574..3750)
                     /locus_tag="VGC82_21830"
     CDS             complement(3574..3750)
                     /locus_tag="VGC82_21830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425972.1"
                     /translation="MDKDDFGKLVDQSGLSLTNEQKSVLFEVYPLFQAMIARATPPMP
                     REAEPAVIFMPEVR"
     gene            complement(3992..4972)
                     /locus_tag="VGC82_21835"
     CDS             complement(3992..4972)
                     /locus_tag="VGC82_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0425973.1"
                     /translation="MHNGPVLRVQDLTVRFTRRNTDVAAVNGVDFSLAAGETLTILGE
                     SGSGKSVSLKALMGLLPVYAGVSGKVWLNGREILGLPAAELAKLRGGAVSMIFQEPMA
                     ALDPVYTIGEQIAETIVHHEGCDQRTAMKHALGLLEQVKIPSAARRLKNFPHEMSGGM
                     RQRAMIALALACKPSVLLADEPTTALDVTVQIQILLLLRQLQEEMGMAIVFVTHDVGA
                     AAEISDRIAVMYAGRFIETGTTREVMRSPMHPYTQGLLSSTVHGGMRGRALETIPGSP
                     PNLAELPPGCPFAPRCRYAVDACRGGEIPVVTTATGAMARCVRARELVEA"
BASE COUNT          850 a         1614 c         1670 g          985 t
ORIGIN      
        1 gtgccccggg cgttttgcca aaggcgggat ggagatgagc gctgtggtag ggtggaaaac
       61 cttaagaatt cgctaacgcg ttacgcgcag cggttaccag cgggcaaaat gattttgcgc
      121 cgttaaccaa ttattaggga accgcccggt atgtctcgtc agaccggaaa cccggtgccg
      181 tgtctgcaac cctaaaatag ggttacgctt atcgtgcgac tattcctgtc tgttctgctg
      241 tgcctgctgg tgatggcgtg tgctcccggg cgcggcctgc cggaccttcc gtcaatcggg
      301 ccaggcgctt accggcttgg cccgggcgac gtgatccggt tgattacctt tggcgaggaa
      361 gcactgaccg gcgagttccg cgtcagtgac agcggcatga tcgcgttgcc gctgatcggc
      421 gcggtgccag ccgcggggct atcgccggat gtgctggccg tccatgtcgg cgaggcgctg
      481 gtgaaggcga acctgttgcg cgcgccatcg gtgtcggccg aggtgattgc ctaccgtccc
      541 atttttgtcc tgggtgaggt gagcaagccg ggccagtatc cgtaccagcc gggtatgacc
      601 gtggtaaccg cggtcgccgt agcgggcggc ttcacctatc gggcgttcaa tgattatgct
      661 tccgtggtgc ggacccggga tggagtcgcc atcgagggaa aagcaatccg gcaaagcttt
      721 gtcgagccgg gtgacgttat ctcggtcttt gagcgcaggt tttgaatttt cctctggttg
      781 tcgcggtttt gtcgatgatg ccgtttgcgg ccgtggcgga gtccggcggt tccgcatcgg
      841 cccccctgca tatcatcggc gggccgaata ccggcggctg catcggcggc gccgtcagcc
      901 tgccgccgga tgggccgggt tttcagacca tccattcgaa caacagcgcc ttctggggcg
      961 cgccgcgaac gatttcccac ctggaagcgt tcgggcgcga agccgccggt gcggggctgc
     1021 cgacgctgct ggtcgaggac atctcccgca tccatggcgg cccgatgccg ggtgggcatg
     1081 tcagccatca gatcggcctg gacgccgacg ttgccctgga catgcgggcg cgtccgccgt
     1141 tgccggcttc ggagcgcgat acgattgtgt tgcgaagcat ggtcaggccg gacggacgtg
     1201 acgtcgatcc cggggcgtgg tcgcctcggg tcaccacgct tctgcgcctg gcggccggtt
     1261 tgccggaggt ggaccgcatt ctggtcaacc cggcgatcaa gcagcagctt tgccgggagg
     1321 tggccggcga ccgatcgtgg ctgcatctga tccggccgtg gtatgggcat gccgcgcata
     1381 tgcatatccg gttccgctgc ccggacggcc agccggactg tatccaggcg ccgcctccgc
     1441 cggccgggga tggctgcgat gcgacgctgc aatggtggtt cgatcagttg gatgcgccgg
     1501 ccaggccgtc gagcccatcc aaggtgccgc cgctgccggc agcctgcacc gcgatcctgg
     1561 cgggtggagg ctgagtttcg agggtcgccg gcaagcggcg gtgcggcccg tcacggttca
     1621 ggacctgctc cgaacaaccg cttcaggtac gatatccagt tcatcgtcgt gggcgaaaac
     1681 cccagccgtc aggcaaacgg ctgaacaatg tgcctcgttc tcgtgtctca gttccggggg
     1741 gtcattccaa ccacgatccc tggcctgtcc cggtcagcaa ggatcggctc ctgacccatc
     1801 cgcagccttg caatcgttca cccgattggc ctggtccagc ggttgacatt tattatgctg
     1861 ttgtggtcgt tgcgacaatg tcatgtcatg tcatgacatg ccttgtgccg ttgccacgac
     1921 tttccggcgt tggaaacaaa gccgcgccag gcacggccat gacacaagcg gcagcccaag
     1981 tttctacgcc ggcttcagcc ggaaagcgtt cagtggttcg tccgcatacg gcctcggtcc
     2041 tcggagcatc gccgcggcgc gatccagatc tttcaccatc gcttccagca tcgacatatc
     2101 gtcgggcttg ggacgcaggc cggtcgcctt cacgcgagcc tcgaacttgc tatctgtcat
     2161 tctgcctccc tcagcttgcc agagcgatgg acatcggcgg acggcggctt cgccaggtcg
     2221 ttgccgtttc ataggcatgg ccaacgcgga acacagttgc ctcggcgaat ggcttgccaa
     2281 cgatctgcat gctgagcggc aatccctcgg cgccgtagcc ggtgcaaatg ctcaaggccg
     2341 gaagcccggt caggttgaac ggcatggtga aattgggctt ctccagcatc gcccatttcg
     2401 gcacttcgtg gattttcggc gcctcgcctg gcgcggaggc ggtgatcagg atgtcgaggt
     2461 ccgccatcgc atcgcgtaac tcgaggcaca gcacccgccg ccgccgcagc gcctggacgt
     2521 aatccgtcgc ttgcagcaac gccccgaacg cgagccggtc gcgcagcaat tcgccatagt
     2581 cgttgaagcg ctccttcagc cagggctcgt gcaccgcgta ggcttctgct gtcaggatca
     2641 accagccggg ggcgttgtag tccatcatcg gcgacagcgt gacgtcgcag atttcggcgc
     2701 ctcgcgagcc gaaaatatcc agcgcgtctt cgatcccctg ccgtgtggct gggctgaccg
     2761 gatgatcgac ctcgtggaaa tgccggataa cgccgatacg aaggcctttg acccccttgt
     2821 cgaggtcggc agtgaagttc gtcaccggac ggtcggcgct ggcgggatcg aacgggtcgt
     2881 ggccggccat tccctgcaac agcagcgcgc aatcctgcgc cgtccaggcc attggaccgg
     2941 tatggtccag cgtttgcgac agcggcagta ccccgcggcg gctggacagc ccgtaggtcg
     3001 gcttgattcc ggcgatgccg cacaatgcgg cggggccgcg gatcgagccg ccggtatcgg
     3061 aaccggtgcc gccgagaacc aggccggccg ccaccgcggc accggtgccc gatgacgatc
     3121 cggcggtaaa atagtccgga ttccacgggt tgcgcgccgg cggccatggc agatcgaacg
     3181 acgggccgcc gatggcgaat tcatgcgtcg ccagcttgcc catcagcacc gtgccggcgg
     3241 cggcccattt gccgacgacg accgaatcct cattcggaac gttgtgttcg agcagcctgg
     3301 aatggccggt cgtgcggatg cccgcggtgt tgtagatgtc cttgtgtccg atcgggatgc
     3361 cgtcgagcgg gcctttcagc gtggccgaca tcatccggcc ctcggcggcc ttcgcgtcgg
     3421 ccagggcgcg atcctcggtg accagcagga aactgtgcaa ggtaggatca agcttcttga
     3481 cccgatcgag gcacatcctg gtcagctcga cgggcgaaag tttcttcgcc gcgatcagcc
     3541 cggaggcctc ggcgatgctg aggaagggtt ccatcaccgc acctccggca tgaagatcac
     3601 cgcgggttcc gcttcgcgcg gcatcggcgg cgtcgctcgg gcgatcatgg cctggaacag
     3661 cggatagact tcgaacagga cagatttctg ctcgtttgtc agtgaaagac cactctgatc
     3721 gaccagcttg ccgaagtcgt ccttgtccat gttcacgtcc tcctgtgccg ctcgttcagt
     3781 cattggtgcg tggatgccgg ggcgcgtccg gccacgccga ataaacacac tggaacggca
     3841 agttgcaaat tggaccgcgg cgaaatcgtc cgcggaagag tctgttccga aatgaccgct
     3901 tcggatacgg tttccagtac gacgttctgg gcggttaccc ggttcaaaac ggttattcga
     3961 catcggaccc tcaggcccgg gcgcacatat tctatgcctc taccagttcg cgggcccgca
     4021 cgcagcgggc catcgccccg gttgccgtcg tcacgaccgg gatttcgcca ccccggcagg
     4081 catcgacggc ataccggcac cgtggcgcga agggacatcc gggtggcagt tcggcaaggt
     4141 tcggtgggct gcctggaatg gtttccagcg cgcgtccccg catgccgcca tgcaccgtcg
     4201 aggacagcag accctgcgtg tagggatgca tcgggctgcg catcacttcg cgtgtcgttc
     4261 cggtttcgat gaagcggccg gcatacatca ccgcaatccg gtcggagatt tcggccgccg
     4321 cgccgacgtc gtgcgtcacg aatacgatcg ccatgcccat ttcttcctgc aactggcgca
     4381 gcagcagcag gatctggatc tgcaccgtca cgtccagggc cgtcgtcggc tcgtcggcca
     4441 gcagcacgct gggtttgcag gccagtgcca gcgcgatcat cgcccgctgc cgcatgccgc
     4501 cggacatctc atgcgggaaa ttcttcaggc ggcgagcggc ggaggggatc ttgacctgct
     4561 ccagcagccc aagcgcgtgc ttcatggcgg tgcgctggtc gcagccttca tggtggacga
     4621 tggtctcggc gatctgttcg ccgatggtat agaccgggtc gagggcggcc atcggttcct
     4681 ggaatatcat cgacaccgcg ccaccgcgca atttcgccag ttcggccgcg ggaaggccga
     4741 gaatttcccg cccgttcaac cagaccttgc cggaaacgcc ggcataaacc ggcaacaagc
     4801 ccatcagcgc cttcaggctg acgctcttgc ccgacccgga ctcgcccagg atcgtcagcg
     4861 tttccccagc cgcgagggaa aaatcgacgc cgttgacggc cgccacatcg gtgttgcggc
     4921 gcgtaaaacg caccgtcaga tcctgcacgc gcaggacggg accgttatgc atgatgcacc
     4981 gccttggcgg attgagcttc agggatgtgg caggcgacca cgtgggtcgg atcggcatcg
     5041 ccatcgaatg gcgccgccaa tggcggcacg gtttccgcgc accgcggctg ggcgaaggcg
     5101 caacgggtgc ggaagcggc
//
LOCUS       DASWQN010001290         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2106206, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001290 DASWQN010000000
VERSION     DASWQN010001290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2106206"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1335
                     /locus_tag="VGC82_21840"
                     /pseudo
     CDS             146..1335
                     /locus_tag="VGC82_21840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611153.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          307 a          435 c          510 g          285 t
ORIGIN      
        1 cgtgcacggg acctgggtct gccaatggaa aagatcaagc gtctagtcgc actctggaaa
       61 gagaggtggc cgggagaatt cagacaggcc gttaagtgga ttgtctgcct gagcgcggcg
      121 aggatgccgc ggatcaggag caagaatgac aaagcgtata cgccggaacc acaacccggc
      181 tttcaaggcg aaggtggcgt tggccgccat caagggggag aagacgctgg ctgagctggc
      241 gcatctgtat gacgttcacc cgaaccagat cacggcgtgg aaggctcacc tgcttgacgg
      301 tgccgccggg gtattcgggt ccggcttggt gaccaaggag gcggcaccgg cgattgatct
      361 caaaacgctg cacgcgaaga tcggcgagtt gacgctggag aacgattttt gtccggtgcg
      421 ctcggcaaag ccggcctgct gagcgcaaag cgatgatcga ccgcacccac gacctgccgc
      481 tggcacgcca ggcggccctg ctgcggctca gccggagcgg cctgtactac gaaccacact
      541 ccgtgccgga gactgagctt gcgatcatgc gtcggatcga cgcgctgcat ctggactacc
      601 cattcgcggg cagccggatg ctgcgtgacc tgttgcgcgg tgaaggcgtc gtcattggcc
      661 gcgagagagt gcggacgatg atgcggcgga tgggcaaggc tggctaaagg ttccagcggc
      721 gaagccgctg gaatagccag atgagggatc gaggcactgt atcgccgccc gaacacgtca
      781 aagccgtgtc caggacacaa gatttacccg tatctgctgc gcggccttgc ggtcgagcgg
      841 gcgaaccagg tctggtgcat ggacatcacc tacattccga tggcacgtgg ttttgtctat
      901 ctcgcggcgg tcgtggactg gttcagccgg cgcgttctgg cgtggcgggt ttcgatcacc
      961 atggaggttg atttttgcct ggaggcggta gaggaggcgc tggccaggta cggcaagccg
     1021 accatcttca atactgatca gggttcgcag ttcaccagcg cgggcttcac cggcctgttg
     1081 ctggacaacg ggattgcgat cagcatggac ggccgcggcg cgtggcggga caatgtcttt
     1141 gtcgagcggt tatggcgatc ggtaaaatac gaagaggtct atctgcgggc ctatgagacc
     1201 gtcaccgagg cccgcaggct gatcggccgg tatctggatt tcttcaaccg caagcggcca
     1261 cattcgagcc ttggcgcgcg aacgccggac gacgcctact tcacccagtt gccgtcgttg
     1321 gcagccgtcg catgaatttc gccgttgctg ttggctcgcc tctccggtcg ggctacgccc
     1381 tccctgcgcg gcgagccaac agcaacacat tacgcaacca accggcaggg gatccactta
     1441 acggaggcga aacgctgtag cgaacaagcc ggccacctct gtcagtagaa ctttgcgcat
     1501 cggcctgcca cgtaggatgg tatctataag gaaggat
//
LOCUS       DASWQN010001291         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1973740, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001291 DASWQN010000000
VERSION     DASWQN010001291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973740"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..1721
                     /locus_tag="VGC82_21845"
     CDS             372..1721
                     /locus_tag="VGC82_21845"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0425974.1"
                     /translation="MRVIPGIRLLLWVLWTVAISGTTPGAIAGTGQPIDMIADVIARV
                     QPSVVRVVVVRPPAKANEPDTKLAGAAASGPMSSSGSGFVIASGLVATNRHVVEDAVS
                     IFVSTADGARYRAEVVGMASKADIALLRIDPNAHLPALTLGDSDKLRTGNAVIAIGSP
                     FGFDNSVTAGIVSSVNRDIMESPFDNYIQTDAAINHGNSGGPLFNLAGEVVGMNSVLF
                     APGAGSVGVGFAIPSNDLQFVFDRLEKYGEVRAGMLPIRTQQVTGLMAEALGAPTSGG
                     ALIAALGPEADRMNGEIQPGDVIVRINDQKVVDPRDLARKVALLPVGDSAVLELCRTG
                     KFMSVKVSTLPLEGAMPSTPANASHPKTLGLLFAAVPGEHPPHDVLLADLDPAGSAAD
                     SGLRKGDIIERVQQQAVSSPSQADMALKSLIDAKKTYAALLVKRDDKESWMPVALPQ"
     gene            1761..2171
                     /locus_tag="VGC82_21850"
     CDS             1761..2171
                     /locus_tag="VGC82_21850"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0425975.1"
                     /translation="MQTVRFDHLHLRSPDPDATARFFETMFDADVTRSVYPPGTLYPG
                     QMRVTMRVGGQKILIAPTHPHDPMSPSPAFPYYGLEHIGFTVEDLDATIAELREKGAD
                     VAVGPLTRDAGTYLAFIRGPEGIMVELVQQRRQA"
     gene            2296..3279
                     /locus_tag="VGC82_21855"
     CDS             2296..3279
                     /locus_tag="VGC82_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0425976.1"
                     /translation="MISRRALGIASLAIPSLAIPSLVTRPARAAKVEEIRIDWATYNP
                     VSLVLRDQKLLETEFAKDRIAIRWVQSAGSNKALEFLNAGSLDFGSTAGAAALIAKVN
                     GNPLKTIYVFSRPEWTALVTQGGSSIKAVADLKGQRIAVTRGTDPHIFLVRALAEAGL
                     SEKDVKLVLLQHADGRTAMERGDVAAWAGLDPMMAAAEVESGARLFYRKPEANTWGVL
                     NVREEFAAANPALVLRVLATYETARKWAVAHPAELGAALEAAAKLPQPVIARQLQRTD
                     ISSGQIGEKQAATIIEAGKALQSAGVLDGSLDMAAVTNGMIDPSFCRTIGA"
     gene            3358..>4118
                     /locus_tag="VGC82_21860"
     CDS             3358..>4118
                     /locus_tag="VGC82_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0425977.1"
                     /translation="MKPGNWLPGLALPVGLALLWEIAVRTGVAPGRLMPPPSRLAQTA
                     WSLARSGELQTHVVATMTRVLLGFAIGAATGTLMGMLNGASTWAARLLDPSIQALRAI
                     PSIAWVPLFILWLGIFETSKLALIALGVFFPVYLGILTAIQSVDRKLIEVGRVFGLSR
                     LGIALRITWPAVMPTWIASLRTGLGLGFMFVVAAELMGASEGLGYLLLDGQQMGRADS
                     ILVAMIVFALLGKLSDSVLVALSRPVLRWQDTNRI"
BASE COUNT          725 a         1281 c         1336 g          776 t
ORIGIN      
        1 ccgaaacgaa gcgtcacgcc tcgtacggat aaaagaggcg ccgagttttc cgacattgtt
       61 ccgatgtttc ctccaggcgg cgagccggtg cggcaggttc gcttgttcga ccgccttcac
      121 cttcggacac actggccaag ccaagcgccg gacgcaagcg gctgtcgcga ggaaaaccgc
      181 gaattgccgg gtggatttcg taccgggcga acgcggctgg tgcatccgga taaagtaaca
      241 gaaccgttat gggatgctgc caagaggccc tctcgttatc gagtgccacg atggccctcc
      301 ccgtggtaac ctacccacaa tcggcatcgg tgcttccagg tcggcggcat cgtgcggcag
      361 gaggactcgc cttgcgcgtc atacccggaa tcaggctgct attgtgggtc ctatggacgg
      421 tcgcgatctc tggtacgacc cctggggcga tcgcgggaac cgggcaaccg atcgatatga
      481 tcgcggacgt gatcgcacgc gttcaaccca gtgtcgtgcg agtcgtggtc gtgcgcccgc
      541 ccgccaaagc gaatgagcct gataccaagc tggccggggc cgctgcgtcc ggtcccatga
      601 gcagtagcgg ttcgggtttc gtcatcgcct ccggcctggt cgccaccaac cgtcatgtcg
      661 tggaggacgc ggtttcgatc ttcgtcagca ccgccgatgg cgcccggtat cgagccgagg
      721 ttgtcggcat ggcgtcgaag gcggatatcg cgttgctgcg catcgatccc aacgcccatc
      781 tgccggcact gacgctgggc gacagcgaca agttgcgcac cggaaatgcc gtcattgcga
      841 tcggcagccc gttcgggttc gacaacagcg tgaccgcggg gatcgtcagc agcgtcaacc
      901 gcgacatcat ggaaagcccg ttcgacaatt acatccagac cgatgcggcg atcaatcatg
      961 gcaattcagg cgggccgctg ttcaatctgg ctggcgaggt ggtcggcatg aacagcgtgc
     1021 tgttcgcgcc cggtgccggt tccgtcggcg tcgggttcgc cataccctcg aacgacctgc
     1081 agttcgtttt cgaccggctg gagaaatacg gggaggttcg cgcggggatg ctgccgatcc
     1141 gcacccagca ggttaccggc ttgatggctg aggcgctcgg cgcgcccacg tcaggaggcg
     1201 cactgatcgc ggcactgggc ccggaggctg atcgcatgaa cggcgagatc cagcccggcg
     1261 acgtgatcgt acgcatcaac gatcagaagg ttgtcgatcc gcgcgacctg gcccggaagg
     1321 tggctttgct cccggtgggc gattcagcgg ttctggagct ttgccgaacc ggcaagttca
     1381 tgtcggtgaa ggtatcgaca ttgcctctgg agggcgcgat gccgtcaaca ccggcgaatg
     1441 ccagtcatcc gaagacgttg ggattgcttt tcgccgcggt tccgggggaa cacccgccgc
     1501 acgacgtgtt gctggccgac ctcgacccgg ccggaagtgc cgcggatagc gggttgcgca
     1561 agggcgacat catcgagcgg gtgcaacagc aagccgtctc atcgccaagc caggctgata
     1621 tggcactgaa atcgctgatc gatgcaaaaa agacctatgc agcgttgctg gtcaaacggg
     1681 acgacaagga gtcatggatg ccggtcgcgt tgccgcaata gatcgtcgat tgcctgcacg
     1741 tgagcgccag ggagaggatt atgcaaaccg tccggttcga ccacctccat cttcgcagtc
     1801 cggacccgga cgcaacggcc cgtttctttg agaccatgtt tgacgcggac gtcacacgca
     1861 gcgtgtatcc gccgggcacc ctgtacccgg ggcagatgcg ggtgaccatg agggtcggcg
     1921 gccagaagat cctgatcgcc ccgacccatc cgcacgatcc tatgtctcct tcgcctgcgt
     1981 ttccttacta tggcctggag catattggct tcacggtcga ggatctggac gcgacgatcg
     2041 ccgaactgcg agaaaagggc gcggatgtgg cggttggacc gctgaccagg gacgccggca
     2101 cctacctcgc attcatccgg ggaccggaag gcattatggt ggaattggtt cagcaacggc
     2161 ggcaagcgta ggatttctgc ctcctgtcgg tgaagctccc gacatggagt tctcttttgc
     2221 gggatcccct tgccaaccgg cggaattttt tgctctctgc gctccggaaa ctccggagaa
     2281 aagcctgtga cgaccatgat cagccggcgc gccctcggca tcgccagctt ggccatcccc
     2341 agcctcgcca tccccagcct tgtaacgcgt cccgcgcggg cggcgaaggt cgaggaaatc
     2401 cgcatcgact gggcgaccta caacccggtc agcctggtac tgcgggatca gaagctgctg
     2461 gaaaccgagt tcgccaagga ccgcatcgcg attcggtggg tgcagtccgc cgggtcgaac
     2521 aaggcattgg aattcctcaa cgcgggatcg ctggatttcg gttccaccgc cggggccgcg
     2581 gcgctgatcg ccaaggtcaa cggcaatccg ctcaaaacga tctatgtctt ctcccggccg
     2641 gaatggaccg cattggttac ccagggcggc agctcgatca aggcggttgc cgacctgaaa
     2701 ggccagcgca tcgcggtcac ccgcggcacc gatccccata tcttcttggt ccgcgcgctg
     2761 gcggaagccg ggctgtcgga gaaagacgtg aagctggtgc tgctgcagca tgccgatggc
     2821 cgcaccgcga tggaacgcgg cgacgttgcc gcctgggccg ggctcgatcc gatgatggcg
     2881 gccgccgagg tcgaatctgg tgcccggttg ttttaccgca agccggaagc caacacttgg
     2941 ggggtcctga acgtccgtga ggaattcgcc gccgcgaacc ccgcgctcgt gcttcgcgtg
     3001 ctggcgacat acgaaaccgc ccgcaaatgg gcggttgcgc atccggccga actgggtgcc
     3061 gccctggaag ccgcggcgaa actgccgcag ccagtcatcg cccgacagtt gcagcgcacc
     3121 gatatctcca gcggccagat tggggaaaag caggccgcta cgatcatcga agccggcaag
     3181 gcgcttcagt cagccggcgt tctggacgga agcctggaca tggcggcggt caccaacggc
     3241 atgatcgatc cctcgttctg ccgaaccatt ggggcctgaa tcgggttcag gcagtcgttt
     3301 gctcagtcgc atgatgcaca gggttcgctc agcgggatca tgcgccggtc aaggcgcatg
     3361 aagccgggca actggctacc cggcctggcg cttcccgtgg gcctcgcctt gctatgggaa
     3421 atcgcggttc gaaccggagt ggctcccggc cggttgatgc cgccgccgtc ccggctggca
     3481 caaaccgcat ggtccctcgc ccgctccggt gagctgcaaa cccacgtcgt cgccacgatg
     3541 acgcgcgtgc tgcttggctt cgcgatcggg gccgccaccg gcacgctgat gggaatgctg
     3601 aatggcgcca gcacctgggc agcgcggttg ctcgacccgt ccatccaggc gctgcgggcg
     3661 ataccgtcga tcgcctgggt accgctgttt atcctgtggc tgggtatttt cgagacctcg
     3721 aagttggccc tgatcgcgtt gggcgtgttc ttcccggtct atctgggtat cctgaccgcc
     3781 atccagtcgg ttgaccgcaa gctgatcgag gttggccgcg ttttcggcct gtcgcgcctc
     3841 ggcatcgcgc ttcgcatcac ctggcccgcc gtcatgccaa cctggatcgc gtccctgcgt
     3901 accggcctgg ggctcggttt catgttcgtg gtggccgccg aattgatggg ggcgtccgag
     3961 ggtcttgggt acctgctgct ggacggtcag caaatgggac gagccgattc catccttgtg
     4021 gcgatgatcg tcttcgcgct gctgggaaag ctgtcggata gcgttcttgt ggcactcagc
     4081 cggccggtgc tgcgctggca ggacaccaac aggatcag
//
LOCUS       DASWQN010001292         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_259016, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001292 DASWQN010000000
VERSION     DASWQN010001292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259016"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VGC82_21865"
     CDS             <1..360
                     /locus_tag="VGC82_21865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0425978.1"
                     /translation="LDTKGRPVPGLAPAAKEAGRHVARSIRLRLRGKADKPFRYRHYG
                     NLATIGRNRAIIEFGRLHLKGFLAWMLWGVAHIFFLIGFRNRVVVAVDWLWAYVWFRG
                     NARLITNVDRGDNDAER"
     gene            complement(498..1721)
                     /locus_tag="VGC82_21870"
     CDS             complement(498..1721)
                     /locus_tag="VGC82_21870"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0425979.1"
                     /translation="MDTRPSDRSSSRKRAAAGGCRVSARPAPEAAARRALVLSGGIAL
                     GAFEAGAFAAIEEADDNLPEWITAASIGAVNAAIIAGNRPGERAAALRRFWSCAASDP
                     TPAVTALLGPPPVSGAWRRAYNEAAALQSLTFGRPGMFRPRMMPGFMIGRAPALFDLA
                     PLTARLREVVDFGRLNAGNPRLSIVATDVVSGERVVFDTAHGTRIGAEHVTASGALLP
                     LFAPVEVEGRLLGDGGLASNAPLDLVLDEPGEGALHCIVLELFTLSGPRPRFLSASLG
                     RATDLAFGNQTSRLLEGRAREYQLLALLGRVADRLPPDLREDPEVAAVLRQVREPPQQ
                     TTVVNLHYRPGLDEAGPGKLFDFSSATLAERWAAGENGMREAVQRLLRPERATALAPG
                     LLLHELEAGPDRVPE"
     gene            complement(1727..2776)
                     /locus_tag="VGC82_21875"
     CDS             complement(1727..2776)
                     /locus_tag="VGC82_21875"
                     /inference="COORDINATES: protein motif:HMM:NF004792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0425980.1"
                     /translation="MSLHNDPGVVVITGASSGIGRATAQAFARRGARLVLAARRADML
                     NDAVRECEALGGVACAVPTDVTDQEQVEALGRYALERFGRIDVWFNNAGIAAFGRLES
                     IPMDAWRRVIEINLFGYVHGARVAIRQFREQGHGVLVQNASIVGRTAKPDGTPYAASK
                     FAIRGFSEALRQEVLDQPGIQVCTVLPSVIDTPFFQHAANFSGHDVRAAPPVYTAEEV
                     AETVVGLVEQPQAEAIVGGFGKIAAAQERIAPTFATWFTGRALHRGFLADTPSADTSG
                     ALYEPVRDGRGIAGGWRDNPDSGGPFLAAITSMMALPIEMAMMPLRMADIGMTMAAPI
                     LEAMMPPAARGPVSR"
BASE COUNT          547 a         1022 c          985 g          506 t
ORIGIN      
        1 ctggacacca aaggaagacc tgtcccgggg ctggcaccgg ctgcgaagga agccggccgt
       61 catgttgcgc gctcgatccg cctgcgcctg cgcggcaaag ctgacaaacc ctttcgttac
      121 agacactatg gcaacctggc caccatcggc cgcaaccgag cgatcatcga attcggcagg
      181 ctgcatctca aggggtttct tgcctggatg ctgtgggggg tcgcccacat cttctttctg
      241 attgggtttc gcaatcgtgt ggtcgtggca gtggactggc tgtgggctta tgtctggttc
      301 cgcggcaatg cccgtctgat cacaaacgtc gatcgcggcg acaatgacgc ggagcggtag
      361 tcacgttgtt caggcggttg ccgtcatggt cggtgaaggc cataagcgct gaagcagcat
      421 tcaactggca tccagaaccg gtccaccggt tgatgatagt gtcatggccg tgctcggtgc
      481 ggtcacctgc aatcgtctta ttcaggtacc cgatcggggc cggcctccag ctcatgcagc
      541 agcagcccgg gcgcgagcgc agtggcacgc tccggtctca gaagccgctg aacggcctcg
      601 cgcatgccat tttcaccggc cgcccatcgt tcagccagtg tagcactaga gaaatcgaac
      661 aacttgccag ggcctgcctc atcgagtccg ggccgataat gcaggtttac caccgttgtc
      721 tgctgcggcg gctcgcgcac ctgccggagc accgcggcga cttcgggatc ttcgcgcaga
      781 tccggtggca agcgatcggc gacgcggcct agcagtgcaa gaagctgata ttcccgtgcc
      841 cgtccttcca ggagccgcga tgtctgattg ccgaaagcga gatcggtcgc gcgccccagc
      901 gacgcactca aaaagcgcgg cctcgggccg gacagcgtga agagctcgag cacgatgcaa
      961 tgcaaggcac cctcgccagg ctcatccagt accagatcga gcggcgcgtt agaggccaag
     1021 ccgccatcgc ccagcaaccg cccctccacc tccaccggtg cgaacagagg cagcaacgcg
     1081 ccggatgctg tcacatgttc cgccccgatg cgggtaccat gcgccgtgtc gaaaacgacg
     1141 cgctcgccgc tgaccacgtc cgtcgccacg atgctgagcc gtggattgcc tgcattaagg
     1201 cggccgaaat ccaccacctc gcgcaggcgg gcagtcaatg gcgccaggtc gaacagcgcc
     1261 ggtgcacggc caatcatgaa gccgggcatc atccggggtc gaaacatccc tggtcggccg
     1321 aatgtgaggc tttgcagcgc ggcggcttcg ttgtacgccc gccgccacgc tccgctgact
     1381 ggaggcggcc caagcaacgc ggtgaccgcg ggcgtcgggt cactggcggc acaactccaa
     1441 aaccgccgca acgccgcggc acgttcgccc ggccggttgc cggcgatgat ggcggcattt
     1501 accgcgccga tggaggccgc ggtgatccat tccggcaggt tgtcgtccgc ctcttcaatc
     1561 gccgcgaacg ccccggcttc gaaagcacca agggcgatgc cgccggacag caccagagcc
     1621 cgccgcgccg ccgcttcagg agccggtcgt gccgagacgc ggcacccgcc ggcggccgcc
     1681 cgctttcttg aactgctccg gtcggaaggc cgagtatcca tgccggttac cgtgagaccg
     1741 gtccgcgcgc ggcaggaggc atcatggctt ccaggatcgg cgccgccatt gtcatgccga
     1801 tatcagccat ccgcagcggc atcatggcca tctcgatcgg cagtgccatc atggatgtga
     1861 tggctgccag gaagggcccg ccgctgtccg ggttatcgcg ccagcccccc gcgatgccgc
     1921 ggccgtccct cactggctcg tacaacgcgc ccgacgtatc cgcgcttggg gtgtcggcga
     1981 ggaatcctcg gtgcaatgcc ctcccggtga accaagtggc aaaggtcggc gcgatccgtt
     2041 cctgtgccgc ggcaatcttg ccgaagccac cgacgatcgc ctccgcctgg ggctgctcga
     2101 ccagtcctac aacggtttct gccacttcct cggcagtata gaccggcggg gcggcccgca
     2161 cgtcgtgtcc gctgaagttc gcggcatgct gaaagaacgg cgtgtcgatg acagaaggaa
     2221 gcaccgtgca aacctggatg cccggctggt ccagcacctc ctggcgaagc gcctcggaga
     2281 agccgcggat ggcgaacttg ctggcggcat agggcgtgcc gtcgggcttc gcggtgcgtc
     2341 ccacgatcga ggcattctgc accagcaccc cgtgcccctg ctcgcggaac tgccggatcg
     2401 ccacgcgggc gccatggaca tagccgaaca ggtttatctc gatcacccgc cgccaggcat
     2461 ccatcggaat ggattcaagc cggccgaaag cggcgatccc ggcattgttg aaccagacat
     2521 cgatgcggcc gaagcgctcc agcgcgtagc ggcccagggc ctccacctgt tcctggtccg
     2581 tgacatcggt cgggaccgca cacgcgacgc cgcccagggc ctcgcattca cggacggcgt
     2641 cgttcagcat gtcggcgcga cgggcagcga gcacgagacg ggcgccgcgc cgcgcgaagg
     2701 cctgcgctgt cgcccggccg ataccgcttg acgcgccggt gatgacgacg acgccggggt
     2761 cgttgtggag ggacatgata gctcctgcga gaattcggca atggccgttg aatgccggtg
     2821 cgccggttgg ctaccggccg ggttgcgcaa aggatccgcc aaagatggtt ccgacagggc
     2881 ccgccatggc catgagtggg gttgcatgcg ttcgggcacg ccagaccccg gccgcgggga
     2941 caataacgga cgggtgtcag ggtaaggccc gcaacttggt ggccgttccg tgccgatcaa
     3001 cccaatcccc aagtcagctt gacccgtcaa cggcgatgtg aatggaacac ctgtagagac
//
LOCUS       DASWQN010001293         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_126721, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001293 DASWQN010000000
VERSION     DASWQN010001293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126721"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..809)
                     /locus_tag="VGC82_21880"
     CDS             complement(282..809)
                     /locus_tag="VGC82_21880"
                     /inference="COORDINATES: protein motif:HMM:NF012500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425981.1"
                     /translation="MSSMSAAAFLLPIRIRNRLRWGRSSPKLRPDSNLLVCPAHACGR
                     SRDERWWQPEPRWWCRSRRGDALYVNDGVYGSLADAGTLGFRYPVRLIRPDGDAAGET
                     TGFTLFGPTCDSADVMFGPFMLPANMAEGDWIEIGQLGAYGACLRTAFNGFDRARIIE
                     VADAPPVTEPLRLAA"
     gene            complement(715..1515)
                     /locus_tag="VGC82_21885"
                     /pseudo
     CDS             complement(715..1515)
                     /locus_tag="VGC82_21885"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
     gene            complement(1888..2790)
                     /gene="speB"
                     /locus_tag="VGC82_21890"
     CDS             complement(1888..2790)
                     /gene="speB"
                     /locus_tag="VGC82_21890"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY0425982.1"
                     /translation="MSLTPTYAVPPSFLGIARTDRDAAYIVAGIPMDIGTTNRSGTRE
                     GPAAIRRASRMLTDGDHPEHWVEPARMAVSDIGDFNLALGDIPASLAAIQAQAQPIAH
                     LIALGGEHGITLPLLRALTHRLGRPVALVHFDAHVDTWPDNFGQAFAHGSPFYHAINE
                     GLVDPRRMIQIGIRSPVQKDVMDWTLAKGVTVLSAQDVHVATPAAIAARIAAVVGDVP
                     AYLSFDIDALDPAFAPGTGTPEIGGLASWQAQAILRRLGSVGFAGMDVVEVAPAYDKS
                     EITALAAATMVWEYLALQGAAAMR"
     gene            complement(2865..>3226)
                     /locus_tag="VGC82_21895"
     CDS             complement(2865..>3226)
                     /locus_tag="VGC82_21895"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEY0425983.1"
                     /translation="RGLSGVSDDIRVLLDSKEPLAKLAVDYFVYHIARTAGAQAVSMG
                     GIDGMVFTAGIGEHSVPVRARVLERLAWLGFELDPTANARGEGRITSANSRLAAFVIP
                     TDEERVIARETMALIKP"
BASE COUNT          579 a         1044 c         1093 g          510 t
ORIGIN      
        1 agcacatccg ggacgacgat ggcgccgacg tcgttgtatt cctcgatctg agccttggtg
       61 agcatcgatc cctccgttct tggatcagag gctacaccga ctttctgcga cgaggctaca
      121 gccgagccgg ccaagtcccc gggagcagag acgaattccc gtggttagag tgaaatccgt
      181 gctgatgggc gcacggcttc cactctagcc cttgttttca cgagcatcct tatccggtgg
      241 gtgaccccac ttggtcgcaa tgatgctcta ggctttgaag gtcaggcggc gagacgaagc
      301 ggttccgtaa ccggcggcgc gtccgcgacc tcaatgatgc gggcgcggtc gaagccgttg
      361 aacgcggtgc gcaggcaggc gccgtaggca ccgagttggc cgatctcgat ccagtcgccc
      421 tcggccatgt tggcgggcag catgaacggg ccgaacatca cgtcggcgct gtcgcaagtc
      481 ggcccgaaca aggtgaagcc ggtcgtctcg cccgcggcgt cgccgtcggg acggatcagc
      541 cgcacagggt agcggaaccc cagcgtcccg gcatccgcga ggctgccata cacgccgtcg
      601 ttcacataca gcgcatcgcc gcgcctggac ctgcaccacc accgaggttc cggctgccac
      661 caacgctcgt cccggctccg cccacaggcg tgcgccggac aaaccaagag attcgaatcc
      721 ggcctcaatt tcggcgaaga acgcccccat cggaggcggt tcctgatccg gataggcaac
      781 aggaaagccg ccgccgacat cgacgacatc gatcttcacc ccagccgccg cgatcacctg
      841 cccggccagc gccagcgcgg cgcgccaagc cagcggatcg aggcactgcg agccgacatg
      901 gaagctgaca ccgacggtcg ccgcacaggt tcgggcttcc cgcagcaagg cagcggcttc
      961 gtccgcccgc gcaccaaact tgccggacag atccagcatc gccccgccct tcggcaaagc
     1021 gatgcgcacg atcagcccca acgcgctggc cacgccggtg gcggcgacct cgttgcggat
     1081 cttggcgagt tcgtcggtgg aatcgagcac gaagtcgcgc acgccgtgct gcgcccaggc
     1141 ttcgcggatc gcgccgcgtg ccttgaccgg gtgcatgaaa tggatgaccg cgtccgcgaa
     1201 catggtccgg atcaggcgga cctcgggcag tgaggcgcag tcgaaatgcc gtatgccgcc
     1261 gttccacagc gcgcgcagca ccgatggatc ggggttgcac ttcaccgcgt acagcacgtc
     1321 gccgggaaag ctttcgacga agtcccgtgc ggtgtcggcc acgacgccgg ggcgaaagca
     1381 atgaatcggc tcgtccggcc gttcggcggc cacgacctca tccaccgtcg ggcggcgaag
     1441 cggagaggca acgagctggc ggcgcagcgg agaaacggca gagcgggtgc gggtaccaaa
     1501 agacatgtcg aacatcctcg cggacagccg aacccgcgag gacgcggttc ggcggtacgg
     1561 ataacgcgcg cagcagcacg cgtgatggaa cggggattgg gccgagaccg cgcgaaaacg
     1621 ccgcggttcc gggtcagacg atgctggtgg tacttcgcat cgcgccggtg cagtcggaca
     1681 taggcgtgtt cctaaacgac cccggcccac ggttggccgg gtctatcgaa aagagcgcgt
     1741 ttacgtcgtt gctgtgcctt atgggtttta cccaaacggg ctcgcggcac gtgcggatgc
     1801 gtcaccgccc cattcagagg cgagagtcgc gatatagggt gaagtgaccg ctgccctgta
     1861 atgcgttttt ggaggaccga cagaccctca gcgcatggct gcggctccct gaagcgctag
     1921 gtattcccag accatggtcg ccgccgccag cgcggtgatt tcagacttgt cataggccgg
     1981 tgcgacctcg accacatcca ttccggcgaa acccaccgaa cccaggcggc ggaggatcgc
     2041 ctgggcctgc caggacgcca ggccgccgat ttcgggcgtg ccggtgccgg gggcgaacgc
     2101 cgggtccagc gcatcgatgt cgaagctgag gtaggcgggc acgtcgccaa cgaccgccgc
     2161 gatgcgcgcg gcaatggcgg ccggggtggc gacatgcaca tcctgcgcgg acagcacggt
     2221 cacgcccttc gcgagggtcc agtccatcac gtccttctgc acgggggagc gaatgccgat
     2281 ctggatcatc cgccggggat cgacaaggcc ttcgttgatc gcgtgataga atggcgaacc
     2341 atgcgcgaat gcctggccga aattatccgg ccaggtatcg acatgggcat cgaaatgcac
     2401 cagcgcgacg ggacggccca gccggtgcgt cagcgcccgc agcagcggca gggtgatgcc
     2461 gtgctcgccg ccgagcgcga tcaggtgggc gattggttgt gcctgcgcct ggatggccgc
     2521 gagcgaagcc gggatgtcac cgagcgcgag gttgaaatcg ccgatgtcgg agaccgccat
     2581 gcgggcgggt tccacccagt gttcggggtg gtctccatcg gtcagcatcc ggcttgcgcg
     2641 gcggatcgca gcggggcctt cccgcgtgcc ggagcggttg gtggtgccga tatccatggg
     2701 gatgccagcg acaatgtaag cggcatcccg atcggtgcgg gcgatcccca ggaagctggg
     2761 cgggacggcg taggtcgggg tgagggacat ggcggcgtga tatacccctc gccgcggccg
     2821 gcacagggca tggcgggaag cggcggatcc aaaggttcaa gacgttacgg tttgatcaac
     2881 gccatcgttt cccgcgcgat gacccgttcc tcgtctgtgg gaatcacgaa ggccgccagc
     2941 cggctgttgg cgctggtgat ccgcccctcg ccgcgagcgt ttgctgtcgg gtccagttca
     3001 aaaccgagcc aggccagcct ttctagcaca cgcgcgcgca ccgggacgct gtgctcgccg
     3061 atgccggcgg tgaacaccat cccgtcgatg ccccccatgc tgacggcttg ggcgccggcg
     3121 gtgcgggcga tgtggtaaac gaagtaatct acggccagtt tcgccagcgg ttccttgctg
     3181 tccagcagga cgcggatgtc gtccgacacg ccggacaggc cgcgca
//
LOCUS       DASWQN010001294         4888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_391243, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001294 DASWQN010000000
VERSION     DASWQN010001294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391243"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VGC82_21900"
     CDS             <1..381
                     /locus_tag="VGC82_21900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425984.1"
                     /translation="PEPYPGLSWNTLTLANEYPGEVRYFAVPMVSVGPLRMELTACAG
                     ARSNVVFLFSSNGLFRLSYRLIADTTCSDTNPAAQEVFARYVPVSPVVALSMRYRTGR
                     TEVVDITDSTAGYLTQIHWRQGGR"
     gene            381..971
                     /locus_tag="VGC82_21905"
     CDS             381..971
                     /locus_tag="VGC82_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425985.1"
                     /translation="MGRQVTDRWFTGLAAWLLFAGRAAEAGPLPFEMQLYVPPVIADS
                     KAPTPCTVTYMVADIANNDLYAARMRIEPSETDAPVNNRLRCPAVMPPRVGLRALDVC
                     ITRTGESKYCVFADMGRSFEREPTINNSAENASRCASDKASDIGVACWTSGKLSVCDV
                     ACGNSPEEAKVSAQARCQDKQQQSCPVTASVPVSGP"
     gene            complement(940..1902)
                     /locus_tag="VGC82_21910"
     CDS             complement(940..1902)
                     /locus_tag="VGC82_21910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425986.1"
                     /translation="MRRILLTCVASLALYGTAFACLLDRPLTLGLLRARIEANLASGE
                     AIRRPKLVILAGSNGPYSHRCETIAPIVGLPCVNAGVAVGIGLDYLFARWKPLLRPND
                     VVYLPMEEAQYVRPRATADLGPDAAIMLRHDRTTLSTLPPRRQVAALFAGDLRGAVMS
                     LIETVLAGSGFVDPRTAATGATNAEGDHIGHTAALAASNRAALAAIAPFHPTASQIAS
                     GYGTAQIVAFLVWAKANGVRVIGGLPTGFIDSPIGDDALAAIRTIYRGHAADFLELPN
                     QGRYPRAAFFDTQDHLNEAAQIAHSIAIAGALSRFMDRIPAPTL"
     gene            complement(1902..2777)
                     /locus_tag="VGC82_21915"
     CDS             complement(1902..2777)
                     /locus_tag="VGC82_21915"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEY0425987.1"
                     /translation="MGLGKKLVLADMFGGFADIGFEAASHGASLTFFEAWYATLSYAL
                     QIYFDFSGYSDMAIGLARMLNIRFPRNFDSPYQATDIADFWRRWHITLGAFLRDYLYI
                     PLGGNRVGKLRQTGNLMATMLFAGLWHGAAWNFIVWGGLHGAFLVIHTQFRRRFRPLP
                     VLIGRALTLLAVVVAWVPFRAASLASTISVLRGMAGLHGATLPRMIVSAVPPLANVAE
                     PVSVMPFLGDARTLSFPEVSACLLLGWLIVLTLPNVHDMTGRARHWALTASFALSIQA
                     VFFAPHIAPFLYFQF"
     gene            complement(2786..3346)
                     /locus_tag="VGC82_21920"
     CDS             complement(2786..3346)
                     /locus_tag="VGC82_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425988.1"
                     /translation="MMFQTVPFIFGFLPVCLGGFFATGRFWGVNGALYWLAACSLFFY
                     AWWNPAHGPLLIASVVGNHLIARKLRNAPASRVWLTAGIAANLAVLGWFKYADFLLHI
                     VAPTAPDLNVTLPLAISFFTFQQIMFLVDTARGGATVSLLPYAAFVTFFPRLIAGPIV
                     RPAEIIPQLTSPASFIRAPRTSPTES"
     gene            3922..4611
                     /locus_tag="VGC82_21925"
     CDS             3922..4611
                     /locus_tag="VGC82_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425989.1"
                     /translation="MVLTASRFVLLAFVLSLACAASARANPIAIGFSALIGTSNSIDT
                     GNVFGEGVGANLRNQTIAGMVTIDPTSLRQVCNLGGACYGDFGAGAISVSFTLNGITS
                     TVVSTGRLGYLAGSSGGLVAIGDRLPGGFNYLAVGAASEDGTIQESIGVLFNRKTAFS
                     TRGGPTAAIDSLGMIGGGLGLVEGGITFMSPIEHLDATIMSIHVAEPAAFYVLGMGLI
                     LLALTRRIVNA"
     gene            4656..>4888
                     /locus_tag="VGC82_21930"
     CDS             4656..>4888
                     /locus_tag="VGC82_21930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY0425990.1"
                     /translation="MTDDLPPNPALLPAGLRDLLPPDAETEASAVEALMTVFAAHGYQ
                     RVKPPLLEFEDSLLAGSGVAVADQTFRLMDPES"
BASE COUNT          934 a         1434 c         1667 g          853 t
ORIGIN      
        1 cccgagccgt atccggggtt gtcgtggaat acgctgacgc tggccaacga ataccccggc
       61 gaggtgcgat attttgccgt gccgatggtt tccgtcgggc cgctgcggat ggaattgacc
      121 gcctgcgccg gcgccaggag caatgtcgtt ttcctgttca gctccaacgg cctgttccgg
      181 ttgtcctacc ggctgatcgc ggacaccaca tgcagcgaca cgaaccccgc ggcgcaggag
      241 gtcttcgcgc gatacgttcc cgtcagcccg gtcgtggcgt tgagcatgcg ctaccggacg
      301 ggcaggaccg aggtggtgga catcaccgat tcgaccgcgg gttacctgac gcagatccat
      361 tggcgccagg gcgggagata atgggccggc aagtgacgga tcgatggttt acgggactcg
      421 ccgcatggct gctgttcgcc ggccgtgccg ccgaagcggg tccgttgcca ttcgagatgc
      481 aactgtatgt gcctccggtc atcgccgaca gcaaggcgcc gactccttgc acggtgacct
      541 acatggtcgc tgatatcgcc aacaacgact tgtatgccgc gcgcatgcgg atcgaacctt
      601 cggaaaccga tgcgccggtg aacaatcgct tgcgctgccc ggccgtcatg ccaccccggg
      661 tcggtttgcg ggcgttggat gtctgcatca cccgaaccgg cgagtcgaaa tactgtgtgt
      721 tcgcggacat gggccgcagc ttcgaacgcg agccgacgat caacaacagc gcggagaatg
      781 cctcacgctg cgcatcggac aaggcgtcgg atatcggcgt ggcgtgctgg acgtcgggca
      841 agttgtcggt ctgcgacgtg gcgtgcggca actcgccgga ggaagcaaaa gtttcggcac
      901 aggcgcgatg ccaggataag caacagcaga gttgcccggt cacagcgtcg gtgccggtat
      961 ccggtccatg aaccgcgaca acgcgcccgc gatggcgatc gaatgggcta tctgcgcggc
     1021 ttcgttgagg tggtcctggg tgtcgaaaaa cgcagcgcgg ggatagcggc cttgattggg
     1081 cagttccagg aaatcggcgg cgtggccgcg gtagattgtc cggatcgcgg ccagggcatc
     1141 gtcgccgatg ggtgaatcga tgaagccggt gggcagtccg ccgatcacgc gcacgccgtt
     1201 cgcctttgcc cataccagga acgcgacgat ctgcgctgtg ccgtaaccgg aagcgatctg
     1261 gctggccgtg gggtggaagg gtgcgatcgc cgcgagtgcg gctcggtttg atgcggcgag
     1321 ggcggcggta tggccgatat ggtcaccttc ggcattcgtg gctcccgtgg cggcggtgcg
     1381 cggatcgacg aagccgctgc ccgccagcac ggtttcgatc aggctcatga cggcgcctcg
     1441 aaggtcgccg gcgaacagcg cggcgacctg gcggcgcggt ggcagcgtgg ataatgtggt
     1501 cctgtcgtgc cgcagcatga tggcggcatc gggaccaaga tcagcggttg cgcgcgggcg
     1561 tacgtactgg gcttcctcca tcggcagata gacgacgtcg ttcggtcgca gcaacggctt
     1621 ccagcgggcg aacaggtagt cgaggccgat gccgaccgcg acaccggcgt tgacgcaggg
     1681 taggccgacg atcggcgcga tcgtctcgca gcgatgggaa taggggccgt tcgatcctgc
     1741 caggatgacc agcttgggtc gccggatggc ctcgcctgac gccaggttcg cctcgatgcg
     1801 cgcccggagg aggccgagcg tgagggggcg gtccagcagg caagcgaagg cggttccata
     1861 aagggcgagc gaggcaacgc aggtgagaag gatgcggcgc atcagaactg gaaatacagg
     1921 aagggggcga tatgcggggc gaagaatacc gcctggatcg acagcgcgaa gctggcggtc
     1981 agggcccagt ggcgggctcg cccggtcatg tcgtgaacgt tcggcagggt cagcacgatc
     2041 agccagccca gcagcaggca ggcggagacc tccgggaagc tgagggttcg cgcatcgccg
     2101 aggaatggca tcaccgaaac cggttcggcc acattcgcga gcggcggcac ggcggagacg
     2161 atcatgcgcg gcaaagtggc gccgtggagg ccggccatgc cgcgaaggac gctgatcgtg
     2221 ctggccaggg atgcggcgcg aaacgggacc cacgcaacga caacggccaa gagggtcagt
     2281 gctcggccga tgaggacggg aagtgggcga aagcggcggc ggaactgggt gtggatgacg
     2341 agaaacgcgc catgcaaccc gccccagacg atgaagttcc acgccgcccc gtgccacagt
     2401 ccggcgaaca gcatggtcgc catcaggttt ccggtctggc gcagctttcc gacgcggttg
     2461 ccgcccaacg ggatgtagag ataatcccgc aggaaggcgc cgagggtgat gtgccagcgg
     2521 cgccaaaaat ccgcaatatc agtggcttgg tagggactat cgaagttccg cgggaagcgg
     2581 atgttcagca tccgggcgag gccgatcgcc atgtcggaat agccggagaa gtcgaaatag
     2641 atttgcagcg cataggatag ggtggcgtac caggcttcga agaaggtcag cgacgcgccg
     2701 tgtgaggcgg cctcgaagcc gatgtcggcg aacccgccga acatgtcggc cagcaccagt
     2761 ttcttcccga gacccaacag aaaaatcagg attccgtcgg cgatgttctc ggcgcgcgga
     2821 tgaacgacgc cggtgacgtc agttgcggaa tgatctccgc tggccggacg atgggaccgg
     2881 cgatcaggcg cggaaagaat gtgacgaagg cggcataagg cagcagggac accgtcgcgc
     2941 cgccgcgcgc ggtatcgacc aggaacatga tctgctgaaa ggtaaagaag ctgattgcca
     3001 gcggcagcgt gacgttcagg tccggcgccg tgggtgcaac gatatgcagg aggaagtcgg
     3061 catacttgaa ccaccccagg acggccaggt tcgccgcaat accggcggtc agccagaccc
     3121 ttgacgcggg cgcattccgt aacttccggg caatgagatg gttgcccacg accgaggcaa
     3181 tgagcaacgg gccatgggcg gggttccacc acgcgtaaaa gaacaggctg cacgctgcca
     3241 gccagtaaag cgcgccgttg acgccccaga aacggccggt cgcgaaaaaa ccgcccaggc
     3301 acaccggcag gaagccaaag ataaatggga ccgtttggaa catcacggcg cggaatatcc
     3361 agcttcaaag gatcggcttg gaactgctgc aagtgcggtc ttcagttcct catgccgggc
     3421 gcgcgccggt agatccgtcg cggtgggcgc aggcccgcca tcgatgcctg ttccagattt
     3481 agcaccgcga gccgtggatg atggcttgcg cccaccacga cggggacggt cctgcctggc
     3541 acgatgggga tggccccacc gccgcccatg atggggatgg tcccgcccat gacggggacg
     3601 gtcccgcgtg gcacgacgag gatggtccag cccaccatga gggggacggt cccgcttggc
     3661 gtgatgttgc cgggagagga tcactcggtt cgtagcggtt atttgtcatc aaacctcggc
     3721 accacagtga gaaaattccg gtctatcatc gagaccctaa tggaaggtta acaccggctc
     3781 cgtcggcact atttacggat tttctatgta tgcgggctta accatctgaa actataggga
     3841 ttcaagtcgt ggcatgatgc ttgctgttct tcccctgttc cgacttccgc ccgtcctggt
     3901 gggttaaaaa aggggttcgt catggtcctt acagcgtctc gctttgtcct gctggcgttt
     3961 gtcctctcgc tggcatgcgc cgcctcggct cgcgccaacc cgattgccat tgggttctcg
     4021 gccctcatcg ggaccagcaa tagtatcgac acagggaacg tcttcggcga gggcgtaggg
     4081 gcgaatctca ggaaccaaac catcgccggg atggtgacca tcgacccgac ctcgcttcgc
     4141 caggtctgca acctaggcgg cgcctgctac ggcgattttg gcgccggagc gatctcggtc
     4201 agctttactt tgaacgggat cacctcaacc gtcgtctcca ctggcaggct tggctatctt
     4261 gccggctcat cgggtggcct ggtggcgatc ggtgacaggc tcccgggtgg tttcaactat
     4321 ctggcggtcg gcgcggccag cgaagacggg actatacaag aatccatcgg ggtgctgttc
     4381 aacagaaaga ccgcgttctc cacccgcggc ggtccgacag cggcgatcga cagcctgggc
     4441 atgatcggcg gcggattggg attggtggaa ggcggcatta cgttcatgag cccgatcgaa
     4501 catctggacg caacgattat gagtatccat gtcgccgaac cagccgcctt ttacgttctt
     4561 ggcatgggtc tgatcctgtt ggcgctgacg cggcggatcg tcaacgcctg aacaagtgtt
     4621 tcccggggag ccgtttgctg gtagctgacg cctgaatgac cgacgacctt cctcctaatc
     4681 cggctctgct tcccgccggc ctgcgcgacc tgttgccgcc cgacgcggag acggaagcct
     4741 ccgccgtcga ggcgctgatg acggtcttcg ccgcgcacgg ctaccaacgg gtgaaaccgc
     4801 cgttgctgga gttcgaggat agtcttcttg ccggttccgg cgttgcggtg gcggatcaga
     4861 ccttccggct gatggacccc gaaagcca
//
LOCUS       DASWQN010001295         3479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1050437, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001295 DASWQN010000000
VERSION     DASWQN010001295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050437"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="VGC82_21935"
     CDS             complement(<1..1256)
                     /locus_tag="VGC82_21935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEY0425991.1"
                     /translation="MSGTNKDNARAEEFKRATAGVLRAIAEQPDVQVAFQPGPSGVSG
                     KRARLPLPTRALPPQEMAKLRGQSDAIALRLRHHDDGVHAQRMPTRREAKDAYDALEQ
                     VRVEVIGSRFMNGVNANLRAKLSDECESEGYDRMTRKDQLPIDKALALLTRERLSGVA
                     VPEAARRVLNLWRDTLGESADQALSEMMNTQDNQTAFARASRKLLAALDLAEAEVDAE
                     PDDNSEDGEDGGEQSGSEDNSQDGEGDSESEQDSMLGAQPEEMEGEAADDDGSESEDE
                     AAVAEGDDRPGGPQPRRERPNPDSEAIYRAYTRQFDEEVDAEDLCDADELSRLRQQLD
                     QQLQHLQGVISKLANRLQRRLLAQQTRSWEFDLDEGMLDAGRLARVVINPLLALSYKR
                     ELDTEFRDTVVTLLIDNSGSMRGRPIT"
     gene            complement(1355..2371)
                     /gene="cobS"
                     /locus_tag="VGC82_21940"
     CDS             complement(1355..2371)
                     /gene="cobS"
                     /locus_tag="VGC82_21940"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HEY0425992.1"
                     /translation="MNTAVDIAPPRPTSEINLPDTKISAREAFDLDIDMEVPAFSLRT
                     EHVPDLDTAYKFDRETTLAILAGFAYNRRVMIQGYHGTGKSTHIEQVASRLNWPCIRV
                     NLDSHISRIDLIGKDAIVLKDGKQITEFREGILPWALQHACTLVFDEYDAGRPDVMFV
                     IQRVLEVEGKLTLLDQNRVIRPHPSFRLFSTANTVGLGDTTGLYHGTQQINQGQMDRW
                     NIVATLNYLPHAMETGIVMAKMGIDGSKDPATRKRIESMVALADLTRAGFINGDISTV
                     MSPRTVITWAENARIFGDVGFAFRVTFMNKCDEAERSTVGEYYQRCFNEDIPTGGFTT
                     RKFN"
     gene            complement(2455..3048)
                     /locus_tag="VGC82_21945"
     CDS             complement(2455..3048)
                     /locus_tag="VGC82_21945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY0425993.1"
                     /translation="MMRRPTRPRAYAPDPAAPPGRPCDMPGCDQPGEYRAPKSRSSLN
                     DYHWFCLEHVRAYNAGWDFYKGMSPAEVEAQLRADTSWQRPTWPIGSLGHTAWDDDAL
                     KDPLNVLAAARMKTSRRPDPQKPPAELRAPLATLGLEWPTTLDAVKSRYKELAKRHHP
                     DANGGSRASEERLKTINLAYAAVRSRLGTESRVAAAE"
     gene            complement(3272..>3479)
                     /locus_tag="VGC82_21950"
     CDS             complement(3272..>3479)
                     /locus_tag="VGC82_21950"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0425994.1"
                     /translation="EHRQRALVAVAAFTALRLPEQVAKFGQSALNLGLTKTQVIEAIM
                     QTAPLTGFPPALNALAAIGAALAT"
BASE COUNT          576 a         1117 c         1086 g          700 t
ORIGIN      
        1 gtgatcggcc gcccccgcat cgaacccgag ttgtcgatca acagggtgac gaccgtgtcg
       61 cggaactcgg tgtccagctc gcgtttgtag gacagcgcca gcagcgggtt gatcacgacc
      121 cgagctaacc gcccggcgtc cagcatgccc tcgtcgaggt cgaactccca ggatcgcgtc
      181 tgctgcgcca gcaaccgccg ctgcaagcgg ttcgccagct tggagataac cccttgcaaa
      241 tgctgtaact gctgatcgag ctgctgccgc agccgcgaaa gctcgtctgc gtcacacaaa
      301 tcttcggcgt cgacctcctc gtcgaactgc cgtgtatagg cgcggtagat cgcttcactg
      361 tcgggattcg gccgttcgcg ccgcggttgc gggccgccag ggcgatcgtc gccttcggct
      421 accgccgcct catcctccga ctcagaacca tcgtcgtcgg cggcttcgcc ttccatctcc
      481 tccggctgag cccccagcat cgaatcctgc tcggactcgg aatcgccctc gccgtcctgc
      541 gagttatcct ccgacccgga ctgttcgccg ccgtcctcgc catcctcaga attatcatcc
      601 ggttccgcat cgacctcggc ttccgccaga tccagagccg ccagcagctt ccgcgacgcg
      661 cgggcaaatg ccgtttggtt gtcctgggtg ttcatcattt cggacaacgc ctggtcggcg
      721 gattccccga gcgtgtcgcg ccacaggttc aagacccgcc gcgccgcctc gggcacggca
      781 actccggaca gccgctcacg cgtgagcaac gccagtgcct tgtcgatcgg caactgatcc
      841 ttgcgggtca tccggtcgta accctcggac tcgcattcgt ccgacagttt ggcgcgcaaa
      901 ttggcattga cgccgttcat gaagcgcgag ccgatgactt cgacgcgcac ctgctccagc
      961 gcgtcgtagg cgtccttcgc ctcccgccgc gtcggcatcc gctgggcatg aactccgtcg
     1021 tcatggtggc gcaggcgcag cgcgatggcg tccgactggc cgcgcagctt ggccatctcc
     1081 tgcggcggca gcgcgcgggt tggaagcggc aacctggcgc gcttgccgga aaccccggat
     1141 ggcccgggct ggaacgccac ctgaacatcc ggctgttcgg caatggcccg aagcacacct
     1201 gccgttgcgc gcttgaactc ctcggcgcgg gcgttgtctt tgttggtccc gctcatggtt
     1261 gcacccgcat cttctctagc taacccgatt caagccgagg atggcgagcc cacgacagcc
     1321 atgacggtga ctgtccatcc tgcaagcctt acttctaatt gaacttccgc gtggtgaaac
     1381 cgccggtagg aatatcctcg ttgaagcagc gctggtaata ttccccgacg gtggagcgtt
     1441 cagcctcatc acacttgttc atgaacgtga cgcggaaggc gaacccgacg tcgccgaaga
     1501 tacgggcgtt ttccgcccag gtaatcaccg ttcgcggcga catgacggtc gagatatcgc
     1561 cgttgatgaa gcccgcccgg gtcaggtcgg ccagtgcaac catgctctcg atccgtttcc
     1621 gggtcgcggg gtccttcgat ccgtcgattc ccatcttcgc catcacgatg ccggtttcca
     1681 tagcatgtgg caggtaattc aacgtcgcaa cgatattcca gcggtccatc tgaccctgat
     1741 tgatctgttg ggtgccatga taaagtccgg tcgtgtcgcc caggcccacc gtgttggccg
     1801 tcgagaacag ccggaaggac ggatgcggcc ggatgacccg gttctggtcg agcagcgtca
     1861 gcttgccttc gacctccagc acccgctgga tcacgaacat cacgtccggc cggccggcgt
     1921 catactcgtc gaacaccagg gtgcacgcgt gctgcagcgc ccatggcaga atgccttcgc
     1981 ggaattcggt gatctgcttg ccgtccttca ggacgatcgc gtccttgcca atcaagtcta
     2041 tgcggctgat gtggctgtcg agattgaccc ggatgcaggg ccagttgagg cgcgaggcga
     2101 cctgttcgat atgggtcgac ttgccggtgc cgtggtatcc ctggatcatc acgcggcggt
     2161 tgtaggcaaa gcctgccagg atcgccaacg tggtttcccg gtcgaacttg taggcggtat
     2221 ccaggtctgg aacgtgttcg gtgcgaagcg agaacgccgg cacctccatg tcgatatcga
     2281 ggtcaaaagc ctcccgcgcc gagatcttgg tgtccggaag attgatctcc gaggtagggc
     2341 ggggcggggc aatgtctaca gccgtgttca tagtccagcg tttcctgatg caattcctgg
     2401 aaagtctagc gttggcggcg cgtggcgcaa gggcaactcc gcgaggtcgg tttgctactc
     2461 ggctgcggcg accctggatt cggtgcctag acgggatcgg accgccgcat aagcgagatt
     2521 gatggtcttc aagcgttcct cggaggcgcg gctgccacca ttggcatcgg gatgatgtcg
     2581 tttggccaat tccttgtagc gacttttcac cgcgtccagc gtggtcggcc actccaggcc
     2641 taaagtggcc aaaggcgccc gcagttccgc cggcggcttt tgcggatcgg gccgtcgaga
     2701 agttttcatt cgggccgcgg cgaggacgtt gagcgggtct ttcagcgcgt cgtcgtccca
     2761 ggcggtgtgg ccaaggctgc cgatcggcca ggttggccgc tgccacgatg tgtcggcccg
     2821 caactgcgct tcgacctcgg cgggcgacat gcctttgtag aaatcccagc ccgcattgta
     2881 ggcccggaca tgttccaggc agaaccagtg atagtcgttc aggctgctgc gcgacttcgg
     2941 cgcgcggtac tcgccgggct gatcgcagcc gggcatgtcg catggccgac ccggaggcgc
     3001 ggccgggtcg ggtgcgtagg cccggggtcg cgtggggcgt cgcatcatcg tggatctata
     3061 tgtgcgtggg gaccgggcgt tggcaatggt atttgcgtgt ggcgaaacgg ataccgaatc
     3121 ggcggccccg tccggacacg ccgcctgtcc tggacactgc gtaagtccgg ttgtgatttc
     3181 ccgccatata aagggcgtcg ccttccgtgt tcgcgcggct ctagccacct ctcagacgaa
     3241 gctggcgcag cagtcacgaa aaacgtgccg gtcacgtggc taacgccgca ccaatcgccg
     3301 ccagcgcgtt caatgctgga ggaaatccgg ttaatggggc tgtctgcatg atcgcctcga
     3361 tcacctgcgt cttcgtcagg ccaaggttca aggcggattg gccgaatttg gccacctgct
     3421 ccggcagccg cagagcggtg aaggcggcca cggcgaccag cgcccgctgg cgatgctcc
//
LOCUS       DASWQN010001296         4512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1447265, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001296 DASWQN010000000
VERSION     DASWQN010001296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447265"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..969)
                     /locus_tag="VGC82_21955"
     CDS             complement(142..969)
                     /locus_tag="VGC82_21955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0425995.1"
                     /translation="MDRIEKHGLHMPRMGLGTYRMTGAVCQAAVESALSLGYRHIDTG
                     QMYANQDAVGAGMAASGIARGDVHLTSKAHHSIQSPDEIRAAFAESLRLLRTDYLDLY
                     LLHWPRPGTDLPSALETLMGLREAGLVRQIGVANCTVAHLRQAIEDVRAPIVCNQIEY
                     HVLIDQSPVLDYMRRHSLAVIAHVPLAQGRLAENPALAAIGRRYGASAAQVALAWLLD
                     QLDVAVIPKAARRESQLENLEAVRLRLDDADRTAIAALPKTLRCVNPPFAPAWDSVG"
     gene            complement(972..1874)
                     /locus_tag="VGC82_21960"
     CDS             complement(972..1874)
                     /locus_tag="VGC82_21960"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0425996.1"
                     /translation="MITLSENFRALFYTPFYAAHATGAYAKAGVEVDFRLSSDPNRTA
                     SALRSGEIEVMWGGPLRVLLTHDADPASEIVCFCDVVARDPFFVIGREPRPSFRPADL
                     SRVRLGTVSEVPTPWLCLQDDIRRDGVDPAVVNRATDGTMAANADALRTGTLDAVQVF
                     QPYAEELLASGAGHLWYAAANRGLTAYTTLVTRRSVLEQKRDELLAMVQAMHWTLGWI
                     RDTPGAEIHRVLASYFPDVPAAIFAAAIDRYRALELYGHDPVTRREGFDRLAAAMRSG
                     GVLHRAIPFEECIDNGLAEQVVGA"
     gene            1945..2913
                     /locus_tag="VGC82_21965"
     CDS             1945..2913
                     /locus_tag="VGC82_21965"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0425997.1"
                     /translation="MRIVLIGVSHWHTPFFLSPCLEMPDVAIVGVCDPDVARAEPYGA
                     KAGCKVFTDYREMCSALRPDFAFALAQHRDMAELARFLIGERIPFAMEKPCAINAAEA
                     AEIAALAQAAGVFAAVPYVIRYSPLVETVLSMNEPVQYAMFKFIGGMIDRYHQQRVEW
                     VIDRKASGGGPLLNLGVHFMDLCRVLLQGAELTVTGAMMSNHTAHLDIEDHAVVLMQG
                     GGASCMVETGYLYPAPNSVFDLHYSIRTENHYFAARDDSTLEIVTNDRRRTTREMKLT
                     NVHFYPVFVRDTLQRLQDGRKPIAGLADNAAAVALVEAAYAMSPLR"
     gene            complement(2966..3739)
                     /locus_tag="VGC82_21970"
     CDS             complement(2966..3739)
                     /locus_tag="VGC82_21970"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0425998.1"
                     /translation="MAYSPFDLTGKVALVTGGNSGIGLGMALAMAQAGADIALWGTNA
                     AKNAAAAETLAPTGRKVLTLECDVGDEAAVEAAFARTVETLGRIDGCFANAGVSGRGG
                     KSFLEMTSAEWHRVTRVNLDGAFYTFRTAAKHMVERGGGGVLVGTASLAAIEAAPRSE
                     HYAATKGGMISMVKAMAVEFARYDVRAHSILPGWIETNMTANAVQTEGFKTKVLPRVP
                     MRRWGVGDDFGGIAVYLMSSASKYHTGDTFVIDGGYSLF"
     gene            3838..>4512
                     /locus_tag="VGC82_21975"
     CDS             3838..>4512
                     /locus_tag="VGC82_21975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0425999.1"
                     /translation="MILTADSDVARSTSRRKTANGWLTPFIGKNRNTLKENEAAPSAE
                     GLYPMAFLVEKEAEAVVRPHFHQADQYQVVVQGSGRLGVHDIASIAVHYTDAYSAYGP
                     IVAAGEGVSWFTLRNAWDPGARYMPAERQQLRAARAKFQHREATCGPLPPLTERELAA
                     LPAACGSAVIQETADGLGTWRFTVPGDGTVTGPAPSTGGGQFWLVSSGSASVAGGALL
                     PVQSCIF"
BASE COUNT          821 a         1487 c         1405 g          799 t
ORIGIN      
        1 cccggcttcg cctgtggtgc tctccgcggt tcccggaaag cctccgtgca atgacgcttc
       61 cgatcgccgg cagcaagcga taatgcgcta tagtctgcgc agatcacgcc gttcgacctt
      121 tcggtcctgc ggcgatccgc tttatccaac tgaatcccag gccggcgcga acggcgggtt
      181 gacgcagcgc agcgtcttcg gcaacgccgc gatcgccgtt cggtcggcat cgtccagccg
      241 cagtcggaca gcctccagat tctcgagctg gctctcccgc cgagcggctt tcggtatgac
      301 cgccacatcg agttgatcga ggagccaggc cagcgccacc tgggctgcac tggcgccgta
      361 gcgacgcccg atggcagcca gagccgggtt ttcggcaagc cgtccctgcg cgagcgggac
      421 atgggcgatc accgccagac tatgccggcg catgtagtcc agcacggggg actggtctat
      481 cagcacatga tattcgatct ggttgcagac gatcggcgca cggacgtctt caattgcctg
      541 acgcagatga gccaccgtgc aattcgccac gccgatctgc cggaccaacc cagcctcgcg
      601 cagccccatc agggtctcca gcgcagaagg cagatcggtc ccgggacgcg gccagtgcag
      661 caggtaaaga tccaggtaat cggttcgcag caggcgcagg ctttcggcga aggcggcgcg
      721 gatttcgtca gggctctgga tgctgtggtg ggccttgctg gtcagatgga cgtcgccgcg
      781 cgctatgccg gacgcggcca tgccagcgcc gacagcgtcc tggttggcgt acatctgacc
      841 ggtatcgata tgccggtagc ccagggacag cgccgattcc acagccgcct ggcaaacggc
      901 tccggtcatc cgataggtgc ccagccccat ccggggcatg tgcagcccat gcttttcgat
      961 cctgtccaca gctacgctcc caccacctgc tcagccagtc cgttgtctat acactcttcg
     1021 aacggaatcg cccggtgcag cacgccgccg gagcgcatcg cggcggccag ccgatcgaag
     1081 ccctcgcggc gggtgaccgg atcgtgacca tagagttcca gcgcacggta acgatcgatc
     1141 gccgctgcga agatcgccgc ggggacatcc ggaaagtacg aggccaggac ccgatggatt
     1201 tccgcgccgg gcgtgtccct gatccagccc agcgtccagt gcatcgcctg taccatcgcc
     1261 agcaattcat cgcgcttttg ctccagcacc gaccggcggg tcaccagcgt ggtatatgcg
     1321 gtcagcccgc gattggccgc cgcataccac agatgcccgg cacccgaggc gagcagttcc
     1381 tcggcatagg gctgaaagac ctgcaccgca tccagcgtcc ccgtccgcaa ggcgtctgcg
     1441 ttcgctgcca tcgtgccgtc cgtcgcacgg ttaaccacgg ccggatcgac gccgtcgcgg
     1501 cggatgtcgt cctgcagaca cagccatggc gtcggcacct ccgacaccgt tcccagtcgc
     1561 acgcgggaca ggtcggccgg acgaaacgac ggtcgaggtt cccgcccgat cacgaagaac
     1621 gggtcgcggg cgaccacgtc gcaaaaacac acgatttccg aagccggatc cgcatcgtgg
     1681 gtcagcagca cgcgcagcgg gccaccccac atgacctcga tctcgccgga ccgcagggcg
     1741 gaggcggtcc ggttggggtc ggaggacaaa cggaagtcga cctccacacc cgccttcgca
     1801 taggcgccgg tggcgtgcgc cgcatagaat ggcgtgtaaa acagcgcgcg gaaattctcg
     1861 gacagggtga tcatcgcgga tcctcggctt gcggcagccg ctacgatgcg ccagggagag
     1921 acgacgcaaa agaggggaag cgacatgcgc atcgtgctga tcggcgtctc acattggcat
     1981 acgccattct tcctcagccc gtgtctggag atgccggatg ttgccatcgt cggggtctgt
     2041 gatccggatg tggcgcgggc ggagccctat ggcgccaagg ccggctgcaa ggtgttcacc
     2101 gactaccgcg agatgtgttc agccttacgg ccggatttcg ccttcgccct tgcccagcat
     2161 cgcgacatgg ccgagcttgc tcggtttttg atcggtgaac gcattccgtt cgcgatggag
     2221 aagccttgcg cgatcaacgc tgcggaggct gccgaaatcg ccgcgctggc acaggcggcg
     2281 ggtgtgttcg cggccgtgcc gtatgtgatc cgctatagtc cgctggtcga gacggtgctg
     2341 tcgatgaacg agccggtgca gtacgcgatg ttcaagttca tcggcggcat gatcgatcgt
     2401 taccaccagc agcgcgtcga gtgggtgatc gaccgcaagg cctcgggcgg tggtccgcta
     2461 ctgaacctcg gcgtccactt catggatctg tgccgcgtat tgcttcaggg tgcggagctg
     2521 actgtgacag gcgcgatgat gtcgaaccac acggcgcatc tggacatcga ggatcatgca
     2581 gtcgtgctga tgcaaggcgg cggcgccagc tgcatggtgg aaacaggtta tttgtatccc
     2641 gcaccgaact cggtgttcga cctgcactat tccatccgca ccgaaaacca ttacttcgcc
     2701 gcgcgcgacg acagcacgtt ggagatcgtg accaacgacc gccggcgcac gacgcgggag
     2761 atgaaactga cgaacgtcca cttctacccg gtcttcgtgc gcgacacgtt gcagcggctg
     2821 caggacgggc ggaaacccat cgcaggtctg gccgacaacg cggcggcggt ggcgctggtg
     2881 gaggcggctt acgcgatgtc gccgttgcgg taatcgtgcc agagaaccag ggatcgtgtc
     2941 agagaaccag ggatgatgcc gccgcctaaa acaacgagta cccaccgtcg atcacgaatg
     3001 tatcgccggt atgatatttc gacgccgaac tcatcagata taccgcgata ccaccgaaat
     3061 catcaccgac gccccagcgg cgcatcggca cgcgcggcag caccttggtc ttgaaaccct
     3121 cggtctgcac agcgttggcc gtcatattcg tttctatcca gcccggcagg atcgagtgcg
     3181 cgcgcacatc ataccgggcg aactcgacgg ccatggcttt gaccatagag atcatgccgc
     3241 ccttggtcgc tgcgtagtgt tcgctgcgag gggctgcctc gatcgccgcc agcgatgccg
     3301 tcccaaccag cacgccaccg ccgccacgct ccaccatgtg tttcgccgcg gtgcgaaacg
     3361 tgtagaacgc accatcgaga tttacccggg tcacccggtg ccattcggcg ctcgtcatct
     3421 ccaggaatga cttcccaccc cggccggaga cgcccgcatt ggcaaagcac ccgtcgatcc
     3481 ggcccaacgt ttccaccgtc cgcgcgaaag cggcctcgac cgcggcttcg tcaccgacat
     3541 cgcattccag cgtcagcacc ttgcgtccgg tcggcgccaa tgtttcggcc gcggcggcgt
     3601 tctttgccgc gttggtcccc cagagcgcga tatcggcacc ggcctgagcc atcgccaagg
     3661 ccattcccag gccaatgccg ctgtttccgc cggtaaccag cgccaccttt ccggtcagat
     3721 cgaatggact atacgccacg gtgccgtctc cttgctgggt tgaggcaccg tgtctaacag
     3781 cgaaggttgg caggtgaaca gatcgcggct agtctaagca cagcaggaga tcggaccatg
     3841 atccttaccg ctgacagcga cgttgcacgc agcacatcca gacgcaaaac cgccaacggc
     3901 tggcttaccc cgttcatcgg caagaatcgc aacacgttga aggaaaacga ggctgcgcct
     3961 tccgccgaag gcctttatcc catggcgttt ctcgtcgaga aagaagccga ggctgtggtt
     4021 cgcccgcatt tccaccaggc ggaccaatat caggtcgtcg tgcagggctc cggccggctc
     4081 ggcgtgcacg acatcgccag tatcgccgtt cattataccg acgcttactc tgcctacggc
     4141 ccaatcgtgg cggccggcga gggggtatcc tggttcacac tgcgcaacgc ctgggatccc
     4201 ggcgcgcgct atatgccagc ggagcgccag cagctccgcg cggccagggc aaagttccag
     4261 caccgggaag ccacttgtgg tcccttgccg ccgctgacgg agcgggaact cgctgcgctg
     4321 ccggcggcct gcggaagtgc ggtgatccag gagaccgccg acgggctggg cacctggcgc
     4381 ttcactgttc ccggtgatgg cacggtgacc ggtccggccc catcgaccgg agggggtcag
     4441 ttctggctgg tgtcgtcagg gtccgcttcg gtagccggag gcgcattgct gccagtccag
     4501 tcctgcatct tt
//
LOCUS       DASWQN010001297         1579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_127278, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001297 DASWQN010000000
VERSION     DASWQN010001297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127278"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VGC82_21980"
     CDS             complement(<1..398)
                     /locus_tag="VGC82_21980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426000.1"
                     /translation="MPWLAPLLLLLGLFYLFPLLDVVRLSFTDMTLVGGTYRYSLASF
                     TNLLTSVDFIEMAQTTAIFVATSVAGQLLLGLLIAALLIAGERRALPGAALVRSVVLI
                     GWILPGVVVGIVWKLVLDESPSGILAYLLSL"
     gene            complement(477..1292)
                     /locus_tag="VGC82_21985"
     CDS             complement(477..1292)
                     /locus_tag="VGC82_21985"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0426001.1"
                     /translation="MFYRRDLVPTPPKTWDELLGLAPGLRKNGVIPYVFPGGRGEASL
                     MDHLPMLWTQGGRLINAAGRPVFGEGTNREALIQILAFLRKAVVSGASPVRVVNFRFE
                     ADMYPDILRGNVAMFLGGSWIPKQLHDLGDKAEWSMAAIPTRSPAGPSTVAGGWTYGV
                     FTPDPVKQRLIVDFINFIAAGPEGMIGAVSAQGNLPTRTSVADGDAPYLQMATTKAAV
                     EMLPAARARDTAAIYPAVSADLQVAIADVISGQESPESAIDHAFHDVQRQMAK"
     gene            complement(1314..>1579)
                     /locus_tag="VGC82_21990"
     CDS             complement(1314..>1579)
                     /locus_tag="VGC82_21990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426002.1"
                     /translation="VEAWAIRHPAVKLEVEYLGTDINLGMTQLQERVAAGRAPDVASI
                     DSFFLPRFFDQLQPLDLYVHDVDDFMPFVRAGMHGPDGKLKAL"
BASE COUNT          338 a          513 c          453 g          275 t
ORIGIN      
        1 agtgatagca gataagcaag aatgccggac ggcgattcat caagcaccag cttccagact
       61 atgccgacca caacgcctgg caagatccag ccaatcagca cgacgctgcg gaccagcgcc
      121 gcgcccggca atgctcgacg ctcgccggct attagcaggg ccgcgatcag caagccaagc
      181 agcaactgac cggcgacact tgtggcaacg aaaattgcgg tggtctgcgc catttctata
      241 aagtcgacgg atgtaagcag gtttgtgaag cttgccagcg aatagcgata tgtcccgccg
      301 accaaggtca tgtcggtgaa actcaggcgc accacgtcga gcagcggaaa gaggtagaat
      361 aagccaagca gcagcagcag cggagcgagc cacggcaatg gccggttctc cagcggcggt
      421 gcaggccgtt ttcggtcgat cccgccgctt ggaacaggcg caccacccat ccgtggctac
      481 ttcgccatct gccgctggac atcgtggaac gcatgatcaa tcgcggattc agggctctcc
      541 tgaccggaga tcacgtcggc gatcgccacc tgcaggtcgg ctgagaccgc cggatagatc
      601 gcggcagtgt cgcgtgcccg cgctgcgggc agcatttcga cggctgcttt tgttgtcgcc
      661 atctgcaggt agggcgcgtc gccgtcagcc acactggtcc gggttggcag gttgccctgc
      721 gccgacactg ccccaatcat tccctcggga ccagctgcaa tgaagttgat aaaatccacg
      781 atcagcctct gcttcaccgg gtccggcgtg aacacgccat acgtccagcc gccggcgacc
      841 gtggacgggc cagcgggact gcgcgtcggg atcgctgcca tcgaccactc cgccttgtcg
      901 ccaagatcgt gcagctgctt gggaatccag ctgccgccaa ggaacatcgc aacattgcca
      961 cggaggatgt ccggatacat gtccgcctca aagcggaaat taaccacccg caccgggctc
     1021 gccccggaga cgacagcctt tctcagaaac gccaggatct ggatgagcgc ctcgcggttc
     1081 gtcccttcgc caaacactgg cctgccagca gcgtttatga gccgcccgcc ctgtgtccac
     1141 agcatcggca aatggtccat caggctcgcc tcgccgcgtc cgccggggaa aacgtatggt
     1201 attaccccat tttttcgcag acccggtgcc aaccccagca attcgtccca cgtcttcggc
     1261 ggcgttggta ccagatccct gcgataaaac agggcgcgga catcggtgtt gacctaaagc
     1321 gccttcaact ttccatcggg tccatgcatg cctgcccgca caaaaggcat gaagtcgtct
     1381 acgtcatgaa cataaaggtc gagcggctgg agttggtcga agaaccgcgg cagaaaaaac
     1441 gagtcgatcg aagcgacatc gggcgcccgc cccgccgcaa cccgctcctg aagctgcgtc
     1501 atccccaagt tgatgtcggt gcccagatac tccacctcga gcttgacggc aggatgccgt
     1561 attgcccagg cctcaacgc
//
LOCUS       DASWQN010001298         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1974450, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001298 DASWQN010000000
VERSION     DASWQN010001298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974450"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VGC82_21995"
     CDS             complement(<1..731)
                     /locus_tag="VGC82_21995"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF004783.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY0426003.1"
                     /translation="MTNVAVVGAGLIGRAWSIVFARAGFHVTLWDQFPLQITQAMTFV
                     ADRLPELRAAGLLPDEPEAVLARIHPMHSMWETVRDAEYIQECGPERADIKRPLFEEL
                     ERATRSDAIIASSSSGIPASAFTEQLKTRHRCLIAHPVNPPYLVPLVEICPTPWTDPA
                     VTERTREIMVQAGQVPATVKKEIDGFALNRLQGALLAEAFRLLADDVISPADLDSLVK
                     HGLGLRWSFMGPLETIDLNAPGGLA"
     gene            845..1837
                     /locus_tag="VGC82_22000"
     CDS             845..1837
                     /locus_tag="VGC82_22000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEY0426004.1"
                     /translation="MSNMARVAVLDDWQGVARGAADWTPLQARADVVFFSDAFADADD
                     ATAKLADFDIVLSMRERTPLPASLINRLPKLRMLGMTGARNLSLDTAACTARGIPVCC
                     TQGGGYADSATAELALGLLIAAARGLAVGDANIRAGGFQMGVPVGIGLAGKTLGVIGL
                     GRLGGYMATYAKALRMNVIAWSQNLKAERAAEVGVKLVSKDELMATADAISIHMVLSD
                     RSRGLVGRSDIGRMKQGAILVNTSRGPIIDEAAMLDALHAGKIVAALDVYDREPLPVD
                     HPLRSAPNTVLSPHLGYCVKETWDGFYPQMIENALAFMDGKPVRVTNPEVLKGT"
     gene            complement(1865..>2217)
                     /locus_tag="VGC82_22005"
     CDS             complement(1865..>2217)
                     /locus_tag="VGC82_22005"
                     /inference="COORDINATES: protein motif:HMM:NF027067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0426005.1"
                     /translation="SRTDVDEDKALADLTAAPQWTEVQIEAMTAADNDAWTDDDFSNA
                     VKVYPVQTPEQVRTLRARLGLSQAQFALQFGFTVDTVQQYEQGRRKPSGPASTLLRVI
                     EAEPQAVVRALRRR"
BASE COUNT          396 a          662 c          760 g          399 t
ORIGIN      
        1 tccgctagac cgccgggagc gttcagatcg atcgtttcca gcggccccat gaagctccat
       61 cgcaagccca ggccgtgctt gaccagcgaa tccagatcgg cgggtgaaat gacgtcgtcc
      121 gcgagcagcc ggaatgcctc agccaatagc gccccttgca gacggttcag cgcgaacccg
      181 tcgatttcct tcttcaccgt cgcgggaacc tgaccggctt ggaccatgat ctcccgcgtg
      241 cgctccgtta ccgccggatc ggtccacggc gtcgggcaga tttccaccag cggcaccaga
      301 tagggtgggt tgaccggatg cgcgataagg caccggtgcc gggtcttcag ttgttcggtg
      361 aacgcgctgg cgggaatgcc ggaggacgag gacgcaataa tggcatccga cctcgttgcg
      421 cgctccagtt cctcgaacaa ggggcgcttg atgtcggcgc gctccggccc gcactcctgg
      481 atatattcgg cgtcccgcac cgtttcccac atcgagtgca tgggatggat gcgggcaagg
      541 acggcttcgg gttcgtccgg cagcaggccg gcagcgcgta attccggcag gcggtcggcg
      601 acgaaagtca tggcctgggt gatctgaagc gggaactgat cccacaaggt cacatggaag
      661 cccgcccgag cgaaaacgat cgaccaggct cggccgatca gcccggctcc gacgacagcg
      721 acgttggtca tggtgggttc tcctttgggt gggagatgcc acggccgaac cccaatcggc
      781 aaccgcttgc gcgaccggtg cgggggcgtc aggtttccgc gacgatcaac ggaggaaacg
      841 tgcgatgagc aacatggccc gagtggcggt gctggacgat tggcaaggcg tggcacgggg
      901 tgctgccgac tggacgccac tacaggcccg ggccgatgtg gtgttcttct ccgacgcgtt
      961 cgccgacgcc gacgatgcca cggcgaagct ggcggatttc gacatcgtgt tgtcgatgcg
     1021 ggagcggaca ccgctgcctg catcgctgat caaccgcttg ccgaaactgc gcatgctggg
     1081 gatgactgga gcacggaacc tgtcgttaga tacagcggcc tgtacagcac gagggattcc
     1141 cgtgtgctgc acgcagggcg gcggatacgc cgactcggcg accgcggaac tggcgctggg
     1201 cctgctgatc gcagcggcgc gtggattggc ggtgggtgac gcgaacatcc gggctggcgg
     1261 cttccagatg ggtgtgcccg tggggattgg gttggccggg aagacgctgg gcgtgatcgg
     1321 cctcggccgg cttggcggct acatggcgac gtacgcgaag gcactgcgga tgaacgtcat
     1381 cgcctggagc cagaacctga aggcggaacg ggcggcggag gtcggcgtga aactggtatc
     1441 aaaagatgaa ctgatggcca ccgcggacgc gatcagcatc cacatggtac tgtcggatcg
     1501 cagccgtggg ttggtgggcc gatcggatat cggccgcatg aagcagggcg cgatcctggt
     1561 caatacatcg cgcggcccga tcatcgatga ggcggcgatg ctggacgcgc tgcacgcggg
     1621 caagatcgtc gccgcgcttg atgtgtatga ccgggaacct ttgccggtcg atcatccgct
     1681 gcgatcggcg ccgaacacgg tgttgagccc gcatctgggc tactgcgtga aggaaacctg
     1741 ggatggattc tacccgcaga tgatcgagaa cgccctggcc ttcatggatg gcaagccggt
     1801 acgggtgacg aatccagagg tgctgaaagg cacatagcga tcgtcgggat caggcgcagc
     1861 ggcgctatcg tcgccgcaac gcccggacca ccgcttgcgg ttcggcctcg attacccgca
     1921 gcaacgtcga ggccggcccc gacggcttcc gccgtccttg ttcgtattgc tgtacggtat
     1981 ccaccgtgaa cccgaactga agggcgaact gcgcctggct gaggcctagt ctggcccgta
     2041 gcgtccgcac ctgttcgggc gtctggaccg gatagacctt gacggcgttc gagaaatcat
     2101 cgtctgtcca ggcatcgttg tcggccgccg tcatggcttc gatctgtact tcggtccatt
     2161 gcggggccgc agtcagatcg gccagggcct tgtcttcatc gacgtcagtt ctggaac
//
LOCUS       DASWQN010001299         3454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2369860, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001299 DASWQN010000000
VERSION     DASWQN010001299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2369860"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1951
                     /locus_tag="VGC82_22010"
     CDS             113..1951
                     /locus_tag="VGC82_22010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY0426006.1"
                     /translation="MSGLFQDDPTDQDDAPPVPDGPGLFGEALPVVLPDPVYATPYRV
                     LARKYRPTTFDDLIGQEAMVRTLRNAFAASRVAHAFMLTGVRGVGKTTTARIIARALN
                     CIGPDGTGGPTADPCGVCPNCIGILADRHPDVVEMDAASRTGVDDMREVIEATRFRPM
                     QARTKVFVIDEVHMLSRNAFNALLKTLEEPPPHVKFVFATTEIRKVPITVLSRCQRFD
                     LRRVRIAELSQLFSRIAVKEAVTIEPAALEQIARSADGSVRDGLSMLDQAIAQAEGAI
                     TTAQVVDMLGLADREAVFELMDAVMGGKPAAALSITDRAYERGADLGALLQDLLDLLH
                     TITRLKSIPGLRESQDLPEAERTRGAELADRLNVPVLARAWQMLLKGVGEVEIAPDRR
                     AAAEMVLIRLCHVADMPTPGDLVKRLTSAQPGGPAPFPPGGGGGVRAVANGGAVISAE
                     PVVQAGPRLGTFREVVAWVGEQKEAMLHAHLLHSVHLVRFAPPVIELRPQAEAPRDLA
                     SRLAALLSEATGTRWTIAMSAMTGEPTIAEQGNAADTVRKAAAADHPLVRAILAAFPG
                     ARIDTVHDKTADAYGLPASVPEAPDMPDFAPPDAEYFEEDPMEIDP"
     gene            1948..2271
                     /locus_tag="VGC82_22015"
     CDS             1948..2271
                     /locus_tag="VGC82_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY0426007.1"
                     /translation="MKNLAGMMKQAQQMQEKMQEMQAKLESLELTGEAGAGLVKVTLN
                     GKSEMRRVQIDPKIIDPADAEMLQDLVLAAHRDAKNKIDAASAEEMQKVTGGLQLPPG
                     MKLPF"
     gene            2328..2921
                     /gene="recR"
                     /locus_tag="VGC82_22020"
     CDS             2328..2921
                     /gene="recR"
                     /locus_tag="VGC82_22020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY0426008.1"
                     /translation="MGGPEIDHLIALLSKLPGMGPRSARRAALKMLQQPESRMLPLAA
                     ALAQAARAVRPCADCGNLDSQDPCSICANQQRDRSIICVVEGVGDLWALERAGVHHGL
                     YHVLGGTLSAMSGIGPEDLNVASLISRISNGNVMEVILALGATVDGATTAHWLTDQLR
                     PTGVEITRVGQGVPMGGALDVLDDGTLAAALRTRRAT"
     gene            complement(3260..>3454)
                     /locus_tag="VGC82_22025"
     CDS             complement(3260..>3454)
                     /locus_tag="VGC82_22025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY0426009.1"
                     /translation="GREAAESIQLGIEYAPAPPFDAGSPSTARPELVAAVRARMTSNR
                     VEREARVAKVAATLSSDAAA"
BASE COUNT          590 a         1155 c         1133 g          576 t
ORIGIN      
        1 ggaagcagcc gcaacgggtc accgctcggg tcgtttgtcg gtctcccacc tttcccctat
       61 tggtgctcca agccgacgat aaccccagca gactggcagg tgaccaacaa gcatgtccgg
      121 cctttttcag gacgatccca ccgaccagga cgatgcgccg ccggtgcccg atggccccgg
      181 cctgtttggc gaagcattgc cggtggtgct gccggacccg gtctatgcca cgccatatcg
      241 cgtgctggcg cggaaatacc ggccgaccac cttcgacgac ctgattgggc aggaggcgat
      301 ggtgcgcacc ctgcgtaacg ccttcgcagc cagccgggtc gcccacgctt tcatgctgac
      361 gggcgtgcgt ggcgtcggca agaccaccac cgcccgcatc atcgcccgcg cgctgaactg
      421 cattgggccg gacggtaccg gcggccccac ggccgatccc tgcggcgtat gcccgaactg
      481 catcggcatc ctggccgacc ggcaccccga cgtcgtcgag atggacgccg ccagccgcac
      541 tggcgtggac gacatgcgcg aggtcatcga ggccacccgc ttccgcccga tgcaggcccg
      601 caccaaggtc ttcgtcatcg acgaggtcca catgctcagc cgcaacgcct tcaatgcgtt
      661 gctgaaaacg ctcgaggaac cgccgccgca tgtgaagttc gttttcgcga ccaccgaaat
      721 ccgcaaggtg ccgatcaccg tgctgtcgcg ctgccagcgc ttcgacctgc gccgggtacg
      781 gatcgccgag ctgtcgcagt tgttcagccg catcgcggtt aaggaagcgg tcaccatcga
      841 gccggcagcg ctggagcaga tcgcccgctc cgccgacggg tccgtccgcg acgggctttc
      901 gatgctggac caggcgattg cccaggccga gggtgccatc accaccgcgc aggtcgtcga
      961 tatgctcggt ctcgccgacc gtgaggcggt atttgagctg atggacgcgg tgatgggcgg
     1021 caaacccgcc gctgcattgt cgatcaccga ccgggcgtat gaacgcggcg cggatctggg
     1081 cgcgctgttg caggatctgc tcgacctgct gcacacgata acccggctga aatcgatccc
     1141 tggcctgagg gaaagccagg atctgcctga agccgaacgt acccgcggtg ccgaactggc
     1201 cgatcgtttg aacgtgccgg tgctcgcccg cgcctggcag atgctgctga aaggcgtcgg
     1261 cgaggtggaa atcgcccccg accggcgtgc ggcggccgag atggtgctga tccgactgtg
     1321 ccacgtcgcc gacatgccga cgccaggcga tctggtaaag cggctgacct cggcgcagcc
     1381 gggtggaccg gcgccattcc cgcccggcgg tggtggcggg gttcgtgccg tcgccaacgg
     1441 cggggcggtg atttccgcgg agccggtggt tcaggccggg cctcggctgg gcaccttccg
     1501 cgaagtcgtc gcctgggtag gggagcagaa ggaggcgatg ctgcatgcgc atctgctgca
     1561 ctcggtgcat ctggtgcgct ttgctccccc ggtgatcgaa ttgcgtccgc aggccgaggc
     1621 gccccgcgat ctcgcgtccc ggttggcggc cctgctgtcc gaggcgaccg gcacgcgctg
     1681 gaccatcgcg atgtcagcca tgaccggcga gccgaccatc gccgaacaag gcaatgccgc
     1741 ggacacggtt cgcaaagccg ccgccgcgga tcacccgttg gtgcgtgcta tcctggcggc
     1801 gtttccaggc gcgcgtatcg acacggtgca tgataagacg gcagatgctt acgggctgcc
     1861 ggcgtcggtt cccgaggcgc cggacatgcc ggacttcgca ccccccgacg ccgaatattt
     1921 tgaggaagac ccgatggaga tcgacccatg aagaatctgg ccggcatgat gaagcaggcg
     1981 cagcagatgc aagaaaagat gcaggagatg caggcgaagc tggagagcct ggagctcacc
     2041 ggcgaagcgg gcgccggcct ggtgaaggtt actttgaacg gcaagagcga aatgcggcga
     2101 gtgcagatcg atccaaagat catcgacccg gcggatgccg agatgcttca ggacctggtc
     2161 cttgccgcac accgcgatgc aaaaaacaaa atcgacgctg cctcggccga ggagatgcag
     2221 aaggtgacgg gcgggctgca gcttccgccc gggatgaagc tgccgttttg atgcgccacc
     2281 gcgcttcatg ctcttcgttg tctggtcgga cgctcccgta gatccacatg ggcggccctg
     2341 agatcgacca tttgattgcc ctgctgtcga agttgccggg catgggtccg cgcagcgctc
     2401 gccgcgcggc gctgaaaatg ctgcaacagc cggaatcccg catgttgccg ctggccgcag
     2461 cactcgcgca ggctgcccga gccgtccggc cgtgtgcgga ttgcggcaac ctggacagcc
     2521 aggatccgtg cagcatctgc gcgaatcaac aacgcgaccg ctctattatt tgcgtggtcg
     2581 agggcgtcgg tgacttgtgg gcactggagc gcgccggcgt gcatcacggc ctgtaccacg
     2641 tcctgggtgg caccttgtcc gcgatgtcgg gcatcggccc ggaggacctg aatgtcgcga
     2701 gcctgatcag tcgtatttcc aatggcaacg tgatggaggt gatcctcgcg cttggcgcca
     2761 ctgtcgatgg tgccaccaca gcgcattggc tgacggatca gcttcgcccg acgggcgtgg
     2821 agatcacccg cgtcgggcaa ggcgtcccga tgggcggcgc actggatgtg ctggacgacg
     2881 gcaccctcgc cgccgccttg cgcacacgcc gggcaacgta atcgccgccg caggaagcgt
     2941 tggtccaatg ccgagagaac ccgcgctcgg cgaaattccg ccactgtcgc aggtgatccc
     3001 gttcatcggc gccgcccgtg cccgcgcaat gttcgcttga aacgcagagc gcgccgagaa
     3061 cacgcagatc gcccagaagc gtccgttgca tcaccgacga atgcgccacc cccggcggca
     3121 ccgcgtccaa aaagacgttt ctttcgcagc gacctccgcg ctctcggtga ccgctgcgtt
     3181 gaatcgccca agccagccta cgcacgaacc tcgcagcata accggttatc aaccgcccgg
     3241 ccgctcggtt ccgacgggtt caggccgctg cgtcgctgga caatgtagcc gccaccttcg
     3301 cgacccgcgc ctcccgctcg acccgattac tggtcattcg tgcccgcacc gcggcgacca
     3361 gttccggccg cgcagtgctg gggctaccgg cgtcgaaggg tggggcaggg gcatactcga
     3421 tccctagttg gatgctttcc gcagcctccc ggcc
//
LOCUS       DASWQN010001300         3519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_127499, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001300 DASWQN010000000
VERSION     DASWQN010001300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127499"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VGC82_22030"
     CDS             complement(<1..280)
                     /locus_tag="VGC82_22030"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0426010.1"
                     /translation="MMTARNDAELALVERARGLLPAGGLGNVASDTIIARGRAGRVWD
                     VSGREYVDLLLGSGPMLVGHAHPEVTQAVLEQVPLGTTFFTNNEPAIRL"
     gene            complement(718..3267)
                     /locus_tag="VGC82_22035"
     CDS             complement(718..3267)
                     /locus_tag="VGC82_22035"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEY0426011.1"
                     /translation="MHRIDPTRLDLAREFKAAPLGPHSPDLQKLLKIMRWDPVDGRIV
                     AMQPDQEGPWYLARLSGRKGHPIELFRQRPYATLLEAYWGLFRKRWEDHTGQKLALDD
                     ADERDALPAHGEHTLSATRRPIIGYVDKFSVENGQPIAFKVSTEAPGPYCAEIRRLRC
                     GDHVGVGLKTAVVNAPVNGPYEGRHQPIRTGSFVVIPESADLRPDTFTLIATIWPTIP
                     LKGGQAIMGDWNETTGRGYCLTIDAAGALALIVGDGTGKSAISTNVPLLERHWYIVAA
                     SFDAATGEAWIGQRPLMPYALGDNGSDASRTVSIRPAPLGQFRIAAWTDAAGPAGHYN
                     GKIEQPSLLRRPLEPHERAELIAHAKHPPLDRDLIASWDFSKDIPSTEVSDVSGHAHH
                     GETVNLPTRAMKGWRWDGSEYNWSHKPEHYGAIHFHDDDLYDCGWETDFTYDVPLDLP
                     SRLYCARLTCGDAEDFMPFVVRPPRGKARAALALLLPTASYWAYANTHLGLEWGEGEN
                     VRGMFTAVDSTSLFLHEHPEFGCSLYDRHTDGSGVCYSSRLRPILNMRPRERLWQLPA
                     DTHVIDWLEEKDFAFDIITEDDLEAEGEALLSRYRCVMTGTHPEYPSLRMLDAFAAFQ
                     NQGGRFIYLGGNGFYWRTSYHQTLPGVIEMRRGEDGIRSWLAEGGEYYHGFTGELGGM
                     WRRMGRAPQSVAGTGMTAQGFDRSTHYERTADSFNPRASFIFEGIGADERIGDFGLIG
                     GGAAGWEVDRADAALGTPPHALIVATATDFSSSYHWMKEELTHTHSAINGETCPLVRS
                     DMVFYEAPNGGAVFSVSSIAWAGALAHAGYINNVSRITENVIRRFLDPIPL"
     gene            3337..>3519
                     /locus_tag="VGC82_22040"
     CDS             3337..>3519
                     /locus_tag="VGC82_22040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426012.1"
                     /translation="MLVTLSLQEGPVGEEHSEGIVVGLPIEAGLAADLTPAGMNRSTL
                     SSPLCRLMSSERVTAAL"
BASE COUNT          644 a         1120 c         1076 g          679 t
ORIGIN      
        1 ccagccggat cgccggctcg ttgttggtga agaaggtcgt gccgagcggc acttgttcca
       61 gcaccgcctg cgtcacctcc ggatgggcat gacccaccaa catcggtccc gaccccaaca
      121 gaaggtctac gtattcgcgc ccgctgacat cccacacccg gccagcacgg cccctggcga
      181 tgatggtgtc ggaggctacg ttgccaagcc ctccggccgg cagcagtccc cgcgcccgct
      241 ccaccagtgc cagttcggcg tcattccgtg ccgtcatcat gcgcgacgtc ctttccgggt
      301 cagtagcttc aggcgagtgt gccgtctcgc ctcagtgttg gccaccgatc atctggtcag
      361 cgagctgcat ctcgcggaat aggtgatata tggcccaaca ccgaggagcc tgacaccatg
      421 aaactcccgc tgagcggcgg ctgcatatgc agtgcggccc gctatgagat cacgcaggac
      481 cctatcgatg tctacgcatg ccactgcgcc gactgccagc gcgcgacagt cagtgcgttt
      541 tcaagtggcg tggcagtccc cggcgaagcg ttccgactga cgggaaagga acttcaacca
      601 gcctcgggcg gcgtcaccgc tggcggtcgt tccaaaacac gcgggtagac cgaggaagcg
      661 gcagtcaagc catcgttcac cgacgtggcg tcgcctggca caaaagcgca ggcgcaatca
      721 cagcgggatc gggtcgagga agcgccggat cacgttctcg gtgatgcggg aaacgttgtt
      781 gatgtagcct gcgtgagcca gcgcgccagc ccaagcgatc gagctgacgg agaatacggc
      841 gccgccgttc ggcgcttcgt agaagaccat gtcagatcgg accaacgggc aggtctcgcc
      901 gttgatcgct gaatgggtat gcgtcagctc ctccttcatc cagtgataac tggacgaaaa
      961 gtcagtcgcg gttgcgacga tcagcgcatg tggcggcgtg cccaaagctg cgtccgcacg
     1021 atcgacctcc caccccgccg cgccgccgcc aatcaggccg aagtcgccaa tccgttcatc
     1081 ggccccaatg ccttcgaaaa tgaacgaggc acgggggttg aagctgtccg ctgtgcgttc
     1141 gtagtgggtc gatcgatcaa acccctgcgc tgtcatgccg gttcccgcga ccgactgcgg
     1201 cgctcggccc atgcgtcgcc acatcccgcc cagttcgccg gtgaagccat gataatactc
     1261 accgccctcg gcgagccagg atcgaatgcc gtcctcgcca cgacgcatct cgatcacccc
     1321 tggcagcgtc tgatgatagg acgtgcgcca gtagaagcca tttcctccga ggtagataaa
     1381 gcggccgccc tggttctgga acgccgcgaa ggcatcgagc atgcgcagcg aaggatattc
     1441 aggatgcgtg ccggtcatga cgcagcggta gcgcgacagc agggcctcgc cttcggcttc
     1501 aaggtcgtct tctgtaatga tgtcgaaggc gaagtccttc tcctcgagcc aatcgattac
     1561 atgtgtatcc gccggcaact gccacagtct ttctctgggc ctcatgttca ggatcggacg
     1621 cagccgtgag gagtagcaga cgccgctgcc gtccgtgtgc cggtcgtaca gcgaacatcc
     1681 aaactccgga tgctcatgca ggaacagcga tgtagagtcg acagcggtga acatgcctcg
     1741 cacattctcg ccttcgcccc attccaaacc tagatgcgtg ttggcatagg cccagtaact
     1801 ggcagtcggc agaagcaaag cgagtgccgc acgcgccttg ccgcgcggcg gccggaccac
     1861 gaacggcata aaatcctcgg cgtccccgca tgtcagccgc gcgcagtaaa gtctgctcgg
     1921 gaggtccagg ggaacgtcgt acgtgaaatc ggtttcccag ccgcagtcgt ataggtcatc
     1981 gtcgtggaaa tggatggcgc cgtaatgttc gggcttgtgc gaccagttgt attcgctgcc
     2041 atcccagcgc cagcccttca tggcacgcgt cggaaggttg accgtctcgc catgatgggc
     2101 atggccggac acatcagaca cttcggtgga cgggatgtcc ttgctgaaat cccatgacgc
     2161 gatgaggtcg cggtccagcg gcgggtgttt ggcgtgcgcg atcaactccg cccgttcatg
     2221 cggttcgagc gggcgccgta ggagcgacgg ctgttcaatc ttgccgttgt aatgccctgc
     2281 aggacctgcc gcgtccgtcc aggccgcgat ccggaactgg cccagcggag caggccgaat
     2341 gctcaccgtc cggctcgcgt cgctgccgtt gtcgccgagc gcgtagggca ttagtgggcg
     2401 ctgtccgatc caggcttcgc cagtcgcagc gtcgaaactg gcagcaacga tgtaccagtg
     2461 acgctccagc agcggaacgt tggtgctgat cgcagactta ccggttccgt cgccaactat
     2521 cagcgcgagg gcaccagcgg cgtcgattgt caggcagtag ccgcggccag tcgtctcgtt
     2581 ccagtcgccc ataattgcct ggccgccctt cagcggtatc gttggccaga tggtggcgat
     2641 cagcgtgaag gtatccggtc gaaggtctgc actctctgga attacaacga aagaacccgt
     2701 acggatcggt tggtgccggc cttcataggg accgttgacg ggtgcgttaa cgacggcggt
     2761 cttcagcccg acgccgacat ggtcgccaca gcgcaggcgt cggatttcag cgcagtaagg
     2821 cccgggggcc tcggtgctca ccttgaatgc aatgggctga ccgttctcaa cgctgaactt
     2881 gtcgacgtag ccaatgatcg gcctgcgcgt ggcggacaga gtgtgttcgc catgcgccgg
     2941 cagcgcatca cgttcgtccg cgtcatccag agccagcttc tggcctgtgt gatcttccca
     3001 gcgcttgcgg aacaggcccc agtacgcttc cagcagcgtt gcgtagggcc gctgacggaa
     3061 cagctcaatt ggatgtccct tccgtcccga cagccgcgcc agataccaag gcccttcctg
     3121 gtccggctgc atcgccacaa ttcggccgtc aaccggatcc catcgcatta tcttcagtag
     3181 cttctgcagg tccgggctgt gcggcccaag cggtgccgcc ttgaactccc gcgctagatc
     3241 gagccgtgtt ggatcaatgc ggtgcatcct gcttctctcc tcgctccgcg ccgggcgctt
     3301 cggttcgcca cggcgccttt cgccgcatgt gtgtccatgt tggttaccct tagccttcag
     3361 gaaggccctg tcggcgagga acacagtgaa ggaattgtag tcgggctgcc aattgaggcg
     3421 gggctggcgg ctgacctcac accggctggt atgaatcgtt ctacgctttc atcaccgctg
     3481 tgtcggctga tgtcatcaga gcgcgtcact gccgcattg
//
LOCUS       DASWQN010001301         4752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_785300, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001301 DASWQN010000000
VERSION     DASWQN010001301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785300"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1460)
                     /locus_tag="VGC82_22045"
     CDS             complement(150..1460)
                     /locus_tag="VGC82_22045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0426013.1"
                     /translation="MSYNASIPLSKPLTAADEEATYAKVTWRLMPFLFVCYVVAYLDR
                     VNVGFAKLQMLTDLKFSETAYGLGAGIFFIGYFLFEVPSNVLMYRVGARAWIARIMIT
                     WGIISGMMMFVSSPTHFYILRFLLGVAEAGFFPGIILYLTYWYPSHRRGKMVALFMTA
                     NPVSGVIGGPLSGWILNSFAGLYGWAGWQWLFLIEALPSIVMGIAVFFYLDDGIRKAN
                     WVSEGPKQILEKNIESEAGQKEDHSVFGVFRSGKVWLISLIHFCLVMGVYGIGFWLPS
                     IIRATGVKDAFDVGLLTMIPYGAAIVAMILVSRSADKHRERRWHLAVAAVIGGIGLIF
                     SAIYSDHTMLAMAGLTVATAGIISSLPVFWSLPTAFLGGTAAAAGIALINSVGNLAGF
                     VSPYLIGWIKDVTQSTNIGVYMLAGALFAGAVLVLTVPARLVNK"
     gene            complement(1515..3242)
                     /locus_tag="VGC82_22050"
     CDS             complement(1515..3242)
                     /locus_tag="VGC82_22050"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEY0426014.1"
                     /translation="MSEKKPLRSQAWFGRQDKMGFYYRSWLKNSGVPQDQFEERPVIG
                     ICNTWSELTPCNSHFRDLAEHVRRGVLDAGGFPLEFPVMSLGETMLRPTAMLYRNLAS
                     MDVEESIRGNPLDGVILLMGCDKTTPALMMGACSVDMPTIGVSGGPMLRGIHCGRYVG
                     SGTNTISMSEQLRAGEITLEEYHDAEASMNRSHGHCMTMGTASTMACMVEALGVGMPG
                     NAAIPAVDARRNTLAREAGRRIVAMVHEDLTLSKILTRDAFENAIRLNGGIGGSTNAV
                     IHLIAIARRIGVKLDLDDWDELGRDVNCLVDLMPSGQYLMEDFYEAGGLPAVIREFGE
                     RGLLHKDALTVNGKTLWENNSEAPCWKREVIHSFEQPFKPKAGMAVLRGNLAPDGAII
                     KPTAASPHLMQHTGRAVVFESIEELHHQINDDTLDIDENCVMVLKYCGPKGYPGMAEV
                     GNMPLPPKVLKKGVTDMIRISDARMSGTAYGTVVLHVAPEAAAGGPLALVKTGDLITL
                     DVAGRSLHLAVDEEELARRRAAWQPPPPHTDRGYMKLYVDHVLQANHGCDLDFLVGSS
                     GRPVYRDNH"
     gene            complement(3307..>4752)
                     /locus_tag="VGC82_22055"
     CDS             complement(3307..>4752)
                     /locus_tag="VGC82_22055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0426015.1"
                     /translation="PSAMVLEAVSHAAAFQLTLNPKGMTIASFPANLSRRLIVSATAF
                     LDSIISKVTKRLLPFLLLMYVLSFLDRANVGFAKKAFQIDTGISDAVFAFGAGIFFIG
                     YALFEVPSNLIMHRVGARAWMCRIMVTWGIISAAMAYVHTETMFYTMRFLLGVSEAGF
                     FPGVILYLTYWFPAEARGRVTGIFYFGPPLAFIFGSPLSGILLDLDGALGFKGWQWMF
                     LVEGIMASIVGVWAYFYLDNKPINAKWLNPEEKQALQNAIDTEESIKQSHGPKGVLGV
                     LTNPRVLYLCLIYLLIQMSVYGVIFYLPTQVASLVGQKVGLVVGLVTAIPWLCALAAA
                     YWIPRFSDRTGERRLVAAATLAMAGIGIGLSAVVSTPIIGMIALCLAAAGFIAVQPLF
                     WTFPTSYLAGAAAAGGIGLINALGNMGGFVAPNVRTWAESAFHSNSAGLFILAGTTLI
                     GALLIVGTNRLGLSGQVTATAESRREIVAGA"
BASE COUNT         1085 a         1432 c         1316 g          919 t
ORIGIN      
        1 tgaaatcacg gcgcgttcag cgattcacca ggtagcccgg cacgctcagc acgaagatcg
       61 ccccggcgac caatcgtcag aaacgcatga cgaaaacatg atcggggtac tgacgagcat
      121 gaagacaaat tacggcctcg acaggaagat tacttgttta cgagccgcgc cggcaccgtc
      181 agcaccaaga ccgcgcctgc gaacaacgct cccgcgagca tgtacacgcc tatattagtg
      241 ctctgggtca cgtctttgat ccagccgatc aagtaaggac tcacgaaccc tgccaggttg
      301 ccgaccgaat tgatcaaagc aattcccgcc gcggccgcag tgccgccgag gaaggccgtt
      361 ggcaggctcc agaataccgg caagctggaa atgatgcctg ccgtggcaac ggtaagtcct
      421 gccatcgcca acatcgtatg gtcgctatag attgcgctga agatgagccc gataccgccg
      481 atgacagcgg cgaccgcgag gtgccagcgg cgctcccggt gcttgtcggc actgcggctc
      541 actaggatca tggcaacgat cgcggcgccg tagggaatca tggtgagcaa acccacgtcg
      601 aaggcgtcct tgacccccgt cgctctgatg atcgaaggca gccagaaacc tatcccgtac
      661 acacccatca ccaaacaaaa gtggatcaga ctaatcagcc acaccttgcc actacgaaac
      721 acgccgaaaa cggaatggtc ttccttttgg ccggcctcgc tctcaatgtt tttttcgagg
      781 atctgctttg gcccctcact cacccaattg gccttgcgga tgccgtcatc gagatagaaa
      841 aacacggcga tgcccatcac gatcgacgga agcgcctcga tcaggaacag ccactgccag
      901 ccggcccatc cgtagagtcc tgcgaatgaa ttaaggatcc agccggatag cggtccaccg
      961 atgacgccgg aaaccgggtt ggccgtcatg aataatgcga ccatctttcc gcgccggtgt
     1021 gacggatacc agtaggtcag gtaaaggatg atgccgggaa agaagccggc ttcggcaacg
     1081 cccaggagaa agcgcaggat gtagaaatgc gtcggagagc tgacgaacat catcattcca
     1141 gaaatgatgc cccaggtgat catgatgcgc gcgatccagg ctcgcgcgcc gacgcggtac
     1201 ataaggacgt tactcggcac ctcgaacaga aaatagccga tgaagaagat accggctccc
     1261 aacccgtaag cggtttcgct gaacttcaga tcggtcagca tttgaagttt ggcgaaaccg
     1321 acgttgacgc ggtccagata agcgacgacg tagcagacga acagaaaggg catcaatcgc
     1381 caggtgacct ttgcataggt cgcttcctcg tccgcggccg tcaatggttt gcttagagga
     1441 atgctggcgt tataggacat gtctctctcc tcggattaaa tttcaacacg tcctgagcga
     1501 tgcagcgaat gggatcaatg attgtcccgg taaaccggac ggccactact gccaaccagg
     1561 aagtcgagat cgcagccatg gttcgcctgc aatacatggt cgacgtagag cttcatgtag
     1621 ccgcgatcgg tgtgcggcgg cggcggctgc caggcagccc tgcgccgcgc cagttcctcc
     1681 tcgtccactg cgagatgcag gcttcgtcca gcaacgtcca gtgttatcag gtcgccagtc
     1741 tttaccaacg ccagcggacc cccggccgcg gcctccggtg cgacgtgcag caccaccgtc
     1801 ccgtaagccg ttccggacat gcgcgcatcc gagatccgga tcatgtcggt aacgcccttc
     1861 ttcagcactt tgggtggcag cggcatgttg ccgacctcgg ccattccggg gtagcccttc
     1921 gggccacaat acttcagcac cattacgcag ttctcgtcta tgtccagcgt gtcgtcgttg
     1981 atttggtgat gcagctcctc gatcgactcg aagacgaccg cgcgcccggt atgctgcatc
     2041 aaatgcggcg atgccgcggt cggtttgatg atagcgccgt cgggagccag gtttccacgg
     2101 agtacagcca ttcccgcctt cggcttgaaa ggctgttcga aggaatggat cacttcccgc
     2161 ttccagcagg gtgcctcgct gttattttcc cacagagtct tgccattcac tgtgagcgcg
     2221 tctttgtgca acagcccgcg ctctccgaat tcacggatga cggcgggcag acctcccgcc
     2281 tcataaaaat cttccatgag gtattggccc gatggcatca gatcgaccag acaattcacg
     2341 tcgcgcccga gctcatccca atcgtccaga tcgagcttga cgcctattcg gcgcgcaatc
     2401 gcgataaggt ggatcacggc gttggtcgaa ccgccgatgc cgccgttgag gcgaatggca
     2461 ttttcgaacg cgtcgcgggt cagtattttt gagagcgtga ggtcctcgtg gaccatcgcg
     2521 acgatgcgcc ggccggcctc gcgcgccaac gtattgcggc gagcatccac tgccgggatc
     2581 gcagcgttgc ccggcatgcc gacgccgagc gcttcgacca tgcaggccat cgtcgaagcc
     2641 gttcccatcg tcatgcagtg accgtgcgag cgattcatcg atgcctctgc atcgtgatac
     2701 tcctcgagcg tgatctcgcc ggcgcgcagc tgctcgctca ttgaaatcgt attggtgccc
     2761 gaacccacgt acctgccgca atgaattccg cgcagcatcg ggccgccaga tacgccgatc
     2821 gtcggcatgt cgacgctgca ggcacccatc atcagcgcag gtgtcgtctt gtcgcagccc
     2881 atgagaagaa tcacgccgtc gagcggattg ccgcggattg actcttccac gtccatcgaa
     2941 gcgaggttgc gataaagcat ggccgtcggc cgcagcatcg tttcacccag cgacatgacg
     3001 ggaaactcga gagggaagcc gcccgcatca agcacgccgc ggcgcacatg ctcagccagg
     3061 tctcggaaat gcgaattgca cggcgtgagt tcggaccagg tattgcagat tccgattacc
     3121 ggacgttcct cgaattgatc ctgcggtacc ccgctgtttt ttagccagga gcgatagtaa
     3181 aaacccatct tatcctgccg cccaaaccag gcttggcttc gaagcggttt cttttcactc
     3241 atggccatac tccttttata gatggtaaca aatcttgctc gcaagtatcc gcttgtcgct
     3301 atcgagctat gcgcccgcaa caatttcccg gcgactttcc gccgttgctg tgacttgacc
     3361 gctcaacccg aggcgatttg tcccgacaat cagcaatgcg ccaatcaaag tggttcccgc
     3421 cagaataaac agtccggcgc tattggagtg aaacgcggat tcggcccagg ttctcacgtt
     3481 cggcgcgacg aagccgccca tgttgcccaa agcattgatc aacccgatcc ccccggcggc
     3541 cgcggcgcca gccaaataac tcgtggggaa ggtccagaag agcggctgga ccgcgatgaa
     3601 tccggcagcc gccaggcaca gcgcaatcat tccaatgatt ggcgtactca ccacggccga
     3661 gagtccgata ccgattcccg ccatcgccaa ggttgcggcg gcgaccaaac ggcgctcgcc
     3721 ggtacggtcc gaaaaacgcg gaatccagta ggcggccgcg agtgcgcaaa gccatggaat
     3781 cgcagtaacc aatccgacca ccaggcctac cttttggccg acgaggctgg caacctgcgt
     3841 tggcaggtaa aaaatcaccc cgtacacgct catttgaatc agcagatata tcaggcacag
     3901 gtagagcaca cgcggattgg tcaggacgcc cagtacgccc ttggggccgt ggctctgttt
     3961 aatcgactct tcggtatcga tcgcgttctg gagcgcctgc ttttcttccg gattcagcca
     4021 ttttgcattg atgggtttgt tatcgaggta gaagtacgcc cagacgccga cgatggatgc
     4081 cattatccct tccaccagga acatccattg ccatcccttg aaaccgagcg cgccatccag
     4141 atccagcaat atgcctgaca aagggctgcc gaaaatgaag gcaagcggcg gtccgaaata
     4201 aaatatgccc gtaacgcggc cccgtgcttc cgctggaaac caataggtga gatacaggat
     4261 aacgccgggg aaaaatcccg cttccgacac ccccagcaag aagcgcatcg tgtaaaacat
     4321 cgtttcggtg tgcacgtatg ccatcgcggc ggagatgatg ccccaagtga ccatgatgcg
     4381 gcacatccag gcgcgtgcgc cgacacgatg catgatcagg ttggaaggca cttcgaacag
     4441 cgcatagccg atgaaaaata tgcccgcgcc gaatgcgaac accgcatctg aaatgccggt
     4501 atcgatctga aaggcttttt tggcgaaacc gacgttggcc cggtccagga aggaaagcac
     4561 atacatcagc aaaagaaacg gcagcaggcg cttcgtgacc ttgctgatga tggaatctag
     4621 aaatgctgtg gccgacacga taagtctcct cgataaattg gcggggaatg atgcaatcgt
     4681 catccctttt ggattgagag tgagctgaaa agcggcagca tgcgagacag cctccaacac
     4741 catcgcagac gg
//
LOCUS       DASWQN010001302         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1710692, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001302 DASWQN010000000
VERSION     DASWQN010001302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710692"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VGC82_22060"
     CDS             <1..1344
                     /locus_tag="VGC82_22060"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEY0426016.1"
                     /translation="GRNLRQQVPRRSLGEWKPPGNRPDPVEQIKLSHQGRMEALIPIR
                     VGRMVASPYGFLRGTAVVMAWDVAQLPATGITPVVCGDAHLGNFGFYASPERDLVIDL
                     NDFDEAHPGAWEWDLRRLTASIWVAGRRNGSTEAQCHDSVAACVAAYRQEVSFLAYQP
                     LLMRSYQRLDVDRLHETATEKSLRGEIARAAKRARNRTSDRALPRFTAEIVGRRHIVD
                     EPPLITRVPEAEAQRVAEGLDEYLGTLAPHWRRVLGGYTLLDIAHKVVGVGSVGLRAY
                     VALLEGSSADDVVFLQLKQARRSVLAPFIHGGSAWHAHQGQRVVEYQQALQTVSDPLL
                     GWTTIDGLQYYVRQFRNMKGTVALDAIDAAALTDYAGIVGHLLAKGHARTSGASMIAG
                     YVGGSDQLDKALCRFARAYADQTESDHQALVKAVARGALPVEHIEQGTSRSAFRP"
     gene            1842..3038
                     /locus_tag="VGC82_22065"
     CDS             1842..3038
                     /locus_tag="VGC82_22065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY0426017.1"
                     /translation="MANARFNWDDPLLLEQQLNDGERMVRDAANAYCQERLLPRVTED
                     FRAGQTNPAVFREMGELGLLGPTIPEEYGGHGLSYVGYGLIAREIERVDSGYRSMASV
                     QGSLVMVPIHEFGTEAQKRQYLPKLATGELIGCFGLTEPDHGSDPGGMASRARKVPGG
                     YSLSGSKTWISNSPVADVFIVWAKEVSRDGALGPIRGFVLEKGMKGLSTPEIHGKIGL
                     RTSITGEIVMDGVFCPEENAFPEVRGLKGPFTCLNSARYGIAWGALGAAEDCWFRARR
                     YVLDRKQFGRSLAANQLIQKKLVDMQTEITLGLQGCLRLGRMKDEGTASAEITSIMKR
                     NSCGKALEVARLARDMMGGNGISDEFGVARHLVNLEVVNTYEGTHDIHALILGRAQTG
                     ISAFAN"
BASE COUNT          618 a          942 c          980 g          577 t
ORIGIN      
        1 ggccgcaacc tgcgccagca agttccccgc cgttcactcg gagagtggaa gccacccggg
       61 aaccgcccgg acccggtcga acagatcaaa ctttcgcacc aaggcagaat ggaagcactg
      121 attccgatcc gggtcgggcg catggtcgct tcaccgtatg gcttccttcg cggcaccgca
      181 gtggtgatgg cctgggacgt tgcgcaactc ccggcaaccg gcatcacacc ggtcgtctgc
      241 ggcgatgcgc acctgggcaa cttcggcttc tacgcctcgc cggaacggga tctggtcatt
      301 gacttgaatg atttcgacga ggcccatccc ggcgcgtggg agtgggattt gcgcaggctg
      361 acggcaagca tctgggtggc cggacggcgc aacggatcca ccgaggcgca atgccatgat
      421 tcagtggcag cttgcgtggc cgcgtaccgt caggaggtga gtttcctggc ctaccagccc
      481 ttgctgatgc gctcctatca gcggctggac gtcgaccgcc tgcacgagac ggccaccgag
      541 aagtcgttgc ggggcgagat cgcgcgggct gccaagcgcg ctcgcaaccg caccagcgac
      601 cgcgcactac cccgcttcac ggcagagatc gtgggtcgcc ggcatatcgt ggacgagccg
      661 ccactgatca cccgagtgcc ggaggccgag gcgcaacgcg tggccgaagg actggacgag
      721 tacctgggca ccctggcgcc gcactggcgc cgtgtgctgg gcggctacac ccttctcgac
      781 attgcccaca aggtagtcgg cgtcggaagt gtcgggctgc gtgcctacgt cgcactgctc
      841 gagggcagca gtgccgacga cgtcgtgttc ctgcaactca agcaagcgcg gcgctcggtg
      901 ctggccccgt tcattcacgg cggctcggcg tggcatgccc accagggcca gcgggtcgtg
      961 gaatatcagc aagcgctgca gacggtcagt gatccgctgc tcggttggac cactatcgac
     1021 gggttgcaat actacgttcg gcagttccgt aacatgaagg gcaccgtggc cctcgatgcg
     1081 atcgacgctg ccgcgttgac cgactacgcc ggcatcgtcg gccaccttct ggccaagggg
     1141 catgcccgta caagcggagc ctcgatgatc gccggatatg tcggtggatc ggaccagttg
     1201 gacaaggccc tgtgtcgctt cgcgcgggcc tacgccgacc agaccgaaag cgaccaccag
     1261 gctctggtca aggcggtggc gcgcggcgcg ttgccggtgg agcacataga gcagggcacg
     1321 agccggtccg catttcggcc atagcggcgc ccgccgagca aagaaaacca aaaccgcctt
     1381 ctcgcgtcac acatccgttg cgcttagttc ggtcgctctc aagcgcatgg aagcgatcct
     1441 caaatggatt gccgcacggg tgctttgatg gtttaaaact cgtactcggc ctacatcctg
     1501 aatgtcttgc tatgccggct gtcggaggca tcgatgagat atgcggcgtt gtatttaaaa
     1561 aattgccgcc cgcccagcga gaactttccg aatactttca agattcgact gtcacgaaac
     1621 ccgaatcagc cctctgcatc gttcgtgaag gcaactgaca gttgattttc cgtcgtcaca
     1681 cgctcaaatc gctcaaggtc atgtggcgag atcgagaact cacacttttc ggccattatg
     1741 gagagttctc cataatggcc ggcagccgtc tttccctaat gtttgtgcag accggccgct
     1801 ggatacaatg gcccttcgta gaagctctca ccgcaacctc aatggcgaat gcacgtttca
     1861 actgggatga cccgctgctg ctcgaacaac agttgaacga cggcgaacgc atggtgcgcg
     1921 acgcggcgaa cgcctactgt caggagcggc tgttaccaag ggtgacggag gatttccgcg
     1981 ccggtcagac gaatcctgcg gtgtttcgcg aaatgggcga gctcggcctg ctcgggccta
     2041 cgatcccgga agaatacggc ggacatggcc ttagctacgt cggctacggg ctgattgcgc
     2101 gcgaaatcga gcgcgtcgac tccggctacc ggtcaatggc cagcgtgcag ggttcgcttg
     2161 tgatggtgcc gatccatgaa tttggcacag aggcgcagaa gcgacagtat ttgcccaagc
     2221 tcgctaccgg ggaattgatc ggctgctttg ggctaacgga gcccgaccac ggctccgatc
     2281 ccggtggaat ggccagccga gccaggaagg tacccggcgg ctattcgctc agcggctcca
     2341 agacgtggat cagcaacagc ccggtcgccg acgtgttcat cgtctgggcc aaggaagtca
     2401 gccgagacgg cgcgctcggt ccgatccgtg gtttcgtcct cgaaaagggc atgaagggtt
     2461 taagcacccc tgagatccac ggcaagatcg gcctgcgcac cagcatcacc ggcgagatcg
     2521 tgatggatgg cgtgttctgt cccgaagaaa acgcatttcc ggaagtgcgc ggcttgaaag
     2581 gtcccttcac gtgcctcaac tcggcgcgtt acggcatagc ctggggggca ctcggagcgg
     2641 ccgaagattg ctggttccgt gcacggcgct acgtgctcga ccggaagcag ttcggacggt
     2701 cgctggcggc gaaccaattg attcagaaga agttggtcga catgcagact gagatcacct
     2761 tgggcctgca gggctgcctg cgcctcggcc gcatgaagga cgagggaact gcttcagcgg
     2821 agatcacctc tatcatgaag cgcaattcat gcggcaaggc gctcgaagtc gcccgtttgg
     2881 ctcgcgacat gatgggtggc aatggcatca gcgacgagtt cggggtggcc cgtcacctgg
     2941 tgaatctcga agtggtcaac acctatgaag gcacacacga tatccatgcc ctgatcctcg
     3001 gccgtgcaca gaccggcata tcggcattcg cgaactgacc tgcgagttta gcgtcgagca
     3061 cgactggtcc aaagcctagc agacgaagat cgacatggtg cgaagcgtga gcaactc
//
LOCUS       DASWQN010001303        10170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1448000, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001303 DASWQN010000000
VERSION     DASWQN010001303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10170
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448000"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VGC82_22070"
     CDS             <1..892
                     /locus_tag="VGC82_22070"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY0426018.1"
                     /translation="IKGDLQAGGRLHVRRADIRIPDKMPSSIAVLPVRDANAPPPPPQ
                     PTEPPTTIALNLTIDAPQQVFIRGRGLDAELGGTITIRGTTAKPIPDGGLHLRRGTLT
                     VVGSTLNFTEGTIDFSGAGIADPSVHFVATSTTATIVATLTVSGSAKDPKITLSSVPD
                     MPQDEILAQLLFNTSTSKLSPFQLAEIAAALASLSGATSGFDPLESLRNTFGLDRLSV
                     GSNSSGSPTLEAGRYLTRGVYLGAKQSATGGGTQATVQVDIAKGLKLESTAGSGSTTA
                     TGSTSSSDAASIGLTYQFEY"
     gene            987..1217
                     /locus_tag="VGC82_22075"
     CDS             987..1217
                     /locus_tag="VGC82_22075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HEY0426019.1"
                     /translation="MSTNQKSTPDSNPPSNAEKDPDDWTTGQESMTGAQASYLKTLCE
                     EAGETFDPALTKAEASKMIDALQGKTGRGRTH"
     gene            1522..2751
                     /locus_tag="VGC82_22080"
     CDS             1522..2751
                     /locus_tag="VGC82_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HEY0426020.1"
                     /translation="MPPSLPVMDKLAILADAAKYDASCASSGGAKRNSRDSNGVGSST
                     GNGICHSYTPDGRCVSLLKILLTNACVFDCAYCINRRSSNVRRARFTVDEVVNLTLSF
                     YKRNYIEGLFLSSGIIRSSDYTMESLLRVARTLRVTHSFRGYIHLKTIPDASPELIRQ
                     AGLYADRMSINLEMPTAEALRQFAPEKRIESINATMDDLATHIEDAKSEPKAPKFSPA
                     GQSTQIIVGADSSSDGVILTAAASLYDKYRMKRIYYSAFSPIPDSSRILPARAAPLER
                     EHRLYQADWLIRFYGFSAAEILAGTTDDMLDPQIDPKLAWALRNRGRFPVDINTADRE
                     MLLRIPGLGVRSVDRLIKARTHSRLRLDDLGRLAGSVKRLRAFVVTDDHRPTRLLDIA
                     DLRTIVAPRAEQLPLFG"
     gene            2748..3569
                     /locus_tag="VGC82_22085"
     CDS             2748..3569
                     /locus_tag="VGC82_22085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HEY0426021.1"
                     /translation="MIYTVGIRQPDDLDEFRRAARRLLAASVAPSDVVWSDEQTGTLF
                     AEALPGEELVVSVPRSFRELASAVVCHRDDQRWSLLYQLLWRINQGERSLMHQEADPL
                     VHRLHRMALAIKHDQHRMTAFVRFRGVPDPDGEIFVAWYEPRHHILRRTASFFIDRFA
                     NMRFSVLTPDIALHWDGVTERFISGLTREDAASEDRVEAWWRRYYAATFNPARINTRL
                     MQSHMPQHFWRNLPEAKVILELIGEAGARTDRMIRAPAGGAAERANAAAPAALPA"
     gene            complement(3566..4159)
                     /locus_tag="VGC82_22090"
     CDS             complement(3566..4159)
                     /locus_tag="VGC82_22090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY0426022.1"
                     /translation="MKLYFSPTSPYVRKVMACAIIRGLDGRVQKHPSNPHESPGDLVA
                     DNPLSKVPCLVTDDGLSLFGSQLICEYLDSLGDGLPLFPEHGAPRWRALKFQSLGDGI
                     LDAAVPCRGEQGKPREAARDAQIARFKAAISRTVAVLEADPPHKHIDIGSITVACALG
                     YLDFRFASDRWRDGHPKLAAWYEAFAQNKGLAETAPA"
     gene            complement(4175..4900)
                     /gene="gloB"
                     /locus_tag="VGC82_22095"
     CDS             complement(4175..4900)
                     /gene="gloB"
                     /locus_tag="VGC82_22095"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEY0426023.1"
                     /translation="MTVTASPVPILKDNYAWLLREDSTGATAIVDPADAQPIIEAIEK
                     AGGRLDLILLTHHHADHIAGVDEVRARFNCPVVGALADQHRLPKLDEAVKEGDTVRFG
                     NADARVIDTPGHTRGQINYFFPDGAVLLSGDTLFSLGCGRLIEGTPAEMFDSLHKLAA
                     LPADTLVCCGHEYTESNARFALAVDPDNKALHEFVGKVEQLRAAGQPSVPSVLREELK
                     ANPFLLAPDVASFAAMRAKKDKF"
     gene            4946..5674
                     /locus_tag="VGC82_22100"
     CDS             4946..5674
                     /locus_tag="VGC82_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0426024.1"
                     /translation="MLYSGAMTADAHFAAEFYGSARGAVAARILRERLLAMWPDAAGQ
                     SVLGIGYSMPYLRLWRDRAKRCIALTPVQMGAARWPPGLPNLSCAAEEDSLPFADLTF
                     DRILLVHGLEMAENGRRLLREVWRVLKDDGRLLIVVPNRAGVWAYSENTPFGYGQPYS
                     TGQLGRLLASGLFRIERRDAALWMPPSGLRIVLRSSPLFERGGRKMAPGLAGVTLTEA
                     VKDVYAAMPVRAAPRRRLVLAEAA"
     gene            complement(5783..6142)
                     /locus_tag="VGC82_22105"
     CDS             complement(5783..6142)
                     /locus_tag="VGC82_22105"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0426025.1"
                     /translation="MSLGPASRGTADPTPLSERDEEAAGADWRNALSQWVSQHAYYPE
                     QARRELQEGDAKVRVVAAPNGRVKEVELIGKSGSMWLDLALLSLFRDQHLPPLPHGIS
                     DPIEFNFTMHYMLIRPR"
     gene            6141..6359
                     /locus_tag="VGC82_22110"
     CDS             6141..6359
                     /locus_tag="VGC82_22110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426026.1"
                     /translation="MSIVPGICGLDRDEGDDRGAGSGVPKLKFVGGGNSDDFGVGTGL
                     GAGRMGGGLGAGDGGCGTGTGLGSGGAG"
     gene            complement(6768..7406)
                     /gene="metW"
                     /locus_tag="VGC82_22115"
     CDS             complement(6768..7406)
                     /gene="metW"
                     /locus_tag="VGC82_22115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEY0426027.1"
                     /translation="MSTLDPVRFIAKNLRVDLAVIAGMIAPGSRVLDIGCGDGTLIDH
                     LFQDKNCDARGIEIDMAEVTRAVAHGVPVMHGDADFDLAHYPDGAFDYVVLSRALQAV
                     ERPRAVLREMLRIGTRAVVSFPNFGHWLVRWQLLSTGRMPMTATWDRPWHETPNIHPC
                     TIRDFFVLCEEEGYVVERWLAADEDGRRAPWRRAAGLANLFGEQGVFLLRRR"
     gene            complement(7403..8593)
                     /locus_tag="VGC82_22120"
     CDS             complement(7403..8593)
                     /locus_tag="VGC82_22120"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY0426028.1"
                     /translation="MDQPVTHADAAIFSGHTHVTFDDGIALECGTTLRRHTVAYRTYG
                     TLNADRSNAILVCHALTLDQYVAEEHPVTGKPGWWDAVVGPGRPVDTDRFFVICPNVL
                     GGCMGSTGPLSNRELPDGSEIDEPWGTDFPPVTIRDMVRAQKRLIDHLGIQRLFAVIG
                     GSMGGMQVLQWAASYPEAVFAALPIASASYHSAQNIAFHEVGRQAIFADPDWQNGQYW
                     RSGRIPARGLAVARMCAHITYLSEEALTRKFGRRLQNAPKDSTEAISLFGEMFEVESY
                     LRHQGSTFVQRFDANSYLTITRAMDYFDVAADCGGDLATAFKGTRTRFLLASFTSDWL
                     FPTTESRAVARALNRAGANVSFVEFPTDKGHDAFLLDEPDFHRTLAGFLRGCAVHARL
                     DIGS"
     gene            8733..9632
                     /locus_tag="VGC82_22125"
     CDS             8733..9632
                     /locus_tag="VGC82_22125"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY0426029.1"
                     /translation="MSIPVFTVDDIQASGEEEPAPRDGWRSGGLPALRAELDRIDNAV
                     QDLLIQRAEIVEHVARSGKPAAFRPGREASIIRRLVGRHHGSLPPVTLVRIWRELLAG
                     TTSMQGGFSLAVCDPGHGAPMTQLAREHFGALTPLRAYASPDQALIDVNQGVASVAVL
                     PYPSEAESWWVALLHREPRLHIIGRLPFWKPRPDGFPSVQAVVVASTPADASGEDRTF
                     LGFECDGDVSRSRLSSELAPTGLKWASVVWVRQQGSPVAHILVEVEGYLSPDDVRLAR
                     LGPVLRRPVVLGSYAVPTAGGAG"
     gene            9629..>10170
                     /locus_tag="VGC82_22130"
     CDS             9629..>10170
                     /locus_tag="VGC82_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0426030.1"
                     /translation="MTSPSPRPEILTIHPYVAGESELPGANRTVKLSSNEGAFGVPPS
                     ARAAIAKAAAESYRYPDGGADRLREAIGKRWGLDPRRIVCGAGSDDLLYQFCLSYGGP
                     GRDIVMSAHGFSIYHIAGTYAGSRVIKVPERNLTTDLDAMLAAVSPATRLMFIANPNN
                     PTGSMVPVEEIARVRAALPP"
BASE COUNT         1872 a         3261 c         3230 g         1807 t
ORIGIN      
        1 tatcaagggc gacctgcagg ccggaggcag gctgcatgtc cgccgcgccg acatccgcat
       61 ccccgacaag atgccatcga gcatcgcggt cttgccggtg cgcgatgcca atgcgccgcc
      121 gccaccgccg cagccgaccg aaccgccgac cacgattgcc ctcaatctga cgatcgacgc
      181 gccgcagcag gtcttcatcc gcggccgcgg cctcgacgcg gagctgggcg gcaccatcac
      241 catccgcggc acgacggcca agccaatccc cgacgggggg ctgcacctgc gccggggcac
      301 tcttaccgtc gtcggaagta cgttgaattt caccgaaggc acgatcgatt tctccggcgc
      361 cggcatcgcc gatccgtcgg tgcatttcgt cgcaacgtcc accaccgcca cgatcgtcgc
      421 gacgctgacg gtcagcggca gcgcgaaaga cccaaaaatc accctttcga gcgtgccgga
      481 catgccgcag gacgagatcc tggcgcagct tctgttcaac accagcacct cgaagctcag
      541 cccgttccag ttggcggaga tcgcggcggc attggcgtcg ttgtccgggg cgacatcggg
      601 attcgatccg ttggaaagcc tgcggaacac gttcgggctc gatcgcctgt ccgtcggcag
      661 caacagttcc ggcagcccga ccctggaggc gggacgctac ctcacccggg gcgtttacct
      721 tggcgccaaa cagagcgcga ccggcggagg tacgcaggcg accgtacagg tcgatatcgc
      781 caagggcctg aagctggaat caaccgccgg gtccggctct accaccgcca ccggcagcac
      841 cagcagttcg gatgccgcca gtattggttt gacctaccag ttcgagtact gaacaccttc
      901 tgccctcaga aataggcgcc cgcacgacac aggcgaaaaa actccaacta tgcggaacaa
      961 agcaagacca cccacgttga agccgtatga gcacgaacca gaagtccacc cccgactcca
     1021 accctccatc caatgcggag aaggatcccg acgattggac gacaggccag gagtcgatga
     1081 ccggcgccca ggcatcctat ctgaagacgc tctgcgagga agccggcgag acatttgacc
     1141 cagcccttac aaaggccgag gcatcgaaaa tgatcgacgc ccttcagggc aagaccgggc
     1201 gcggccggac ccactagagc gatctgacgc cggctgaaaa agctgcgggt tcgttcgccg
     1261 cccggccttg atgtgccatg gcggtgcttg gcgtggccac ctacgacatt ttcagcctga
     1321 gtgtgccggc cgaaagtggt ctacctccct gttttgtcac gtggttcgca tgcttcggac
     1381 gttctgccca gcgtgatcac gctgggcaga gaacgctgtt gccagtcacc gtggaaccgc
     1441 tgtagcgccc ggcgcatcga gacgaaaatt agtgacaaaa aatcattgac agtcgggata
     1501 ggtttcgaac atatcaagaa catgccaccc tcccttccag tcatggataa actggcgatc
     1561 cttgccgacg ctgcgaaata cgacgcgtcc tgtgcttcgt ctggtggagc caagcggaac
     1621 tcccgcgaca gcaatggggt aggctccagc accggcaacg gcatctgcca ttcctacacg
     1681 cccgacggac gctgtgtctc gctgttgaaa atcctgctga cgaacgcctg cgttttcgac
     1741 tgcgcgtatt gcatcaaccg gcgttcctcc aacgtgcgcc gggcgcgctt caccgtggac
     1801 gaagtcgtca acctgacctt gtcgttttat aaacgcaact acatcgaggg actgttcctc
     1861 tcctcgggca tcattcggtc gtccgactac accatggaaa gtctgcttcg cgtagccagg
     1921 acacttcgtg taacccattc gttccgcggc tacatccacc tgaaaacgat tcccgacgcg
     1981 agccccgagc ttatccggca agccggcctg tatgccgatc gcatgtcgat caatctggaa
     2041 atgccgaccg ctgaagcact gcggcaattc gcgccggaga agcggatcga atcgatcaac
     2101 gccacgatgg acgatctggc gacccacata gaagatgcta aatcagaacc gaaggccccg
     2161 aaattctctc cggccgggca gagtacccaa attatcgtcg gggcggacag cagcagcgat
     2221 ggagtgatcc tcaccgccgc cgcctcgctc tacgacaaat atcggatgaa acgaatctac
     2281 tactccgcct tctcgccgat ccctgactcc agccgtatcc ttcccgcccg cgcggcaccg
     2341 ttggaacgcg aacacaggct gtaccaggct gattggctga tacgatttta tggcttttcc
     2401 gccgctgaaa tcctcgccgg caccaccgac gatatgcttg atcctcaaat cgaccccaag
     2461 ctcgcgtggg cactgcgaaa tcgcggccgc ttcccggtgg acatcaatac cgccgaccgg
     2521 gaaatgttgt tgcgcattcc cggattggga gtccgctccg tcgatcgcct catcaaagcc
     2581 aggacgcaca gccgtctgcg actggacgac ctggggcggt tggcggggtc ggttaagcga
     2641 ctacgcgcgt ttgtggtgac agacgaccac cggccgacgc gcctgctcga catagccgat
     2701 ctccgaacga tcgtggcccc cagggcggaa caactaccgc tgttcggatg atctacaccg
     2761 tcggcatccg ccaacccgac gatctggacg agttccgccg ggcagcgcga aggcttcttg
     2821 ccgctagcgt tgcgccatcg gatgtcgtct ggagcgacga gcaaaccgga accttgttcg
     2881 cggaagcgct gcccggcgag gaactggtcg tgtcggtacc acgctccttc cgtgagttgg
     2941 cgagcgcggt cgtctgccat cgcgacgacc aacgttggtc gctgctctat cagttgcttt
     3001 ggcgcatcaa ccagggtgag cggtcgctta tgcaccagga agccgaccca ttggtgcacc
     3061 ggctccatcg tatggcgctg gccatcaaac atgatcagca caggatgacc gccttcgtcc
     3121 gttttcgagg ggtgcccgac ccggacggcg aaatcttcgt cgcatggtat gaaccgcggc
     3181 atcacatcct tcgccgcacc gcgtcctttt ttatcgaccg cttcgccaac atgcggttct
     3241 ccgttctgac tccggatatc gccctgcact gggacggagt caccgaacgt tttatttctg
     3301 gattgaccag ggaggacgcc gcctcggagg acagggtaga ggcctggtgg cgccgctact
     3361 acgcagccac gttcaatccc gccaggatca acacgaggct gatgcaaagc cacatgccac
     3421 aacatttctg gcgcaacttg ccggaagcaa aagtcattct tgaactgatc ggggaagccg
     3481 gggcgcggac cgacaggatg attcgggcgc cggcgggggg ggctgcggaa cgcgccaatg
     3541 ccgcggcacc agccgcactt ccggcctaag ccggagcggt ctccgccagt cccttgttct
     3601 gcgcgaatgc ctcataccag gccgccagct tcgggtgccc gtcgcgccag cgatccgaag
     3661 caaagcggaa atccagatat cccagcgcac aagcgaccgt gatcgaaccg atatcgatgt
     3721 gcttgtgcgg cggatcagcc tccagcacag ccaccgttcg gctgatcgct gccttgaacc
     3781 gagcgatctg ggcatcgcga gccgcttccc gcggcttgcc ctgctcgccg cgacaaggca
     3841 ccgcggcatc gagaatccca tcgcccagcg actggaactt cagcgcccgc caccgtggcg
     3901 cgccatgctc ggggaacaac ggcaatccgt cacccaggct gtcgaggtac tcgcagatca
     3961 actgactgcc aaacaatgac agcccatcat cggtgaccag acaaggaacc ttcgaaagcg
     4021 gattgtccgc tacaaggtca ccaggagatt catgcggatt gcttgggtgc ttctgaaccc
     4081 ggccgtccag gccgcggata atcgcgcacg ccatgacttt gcggacgtac gggctggtcg
     4141 gcgagaagta aagcttcacg tcaatgcgtc cctctcagaa tttgtccttc ttcgcacgca
     4201 tcgctgcgaa gctcgccaca tccggggcaa gcaggaatgg gtttgctttc agctcctcgc
     4261 gcagcaccga cggcacgctg ggctgacccg cagcccgcaa ttgctcgact ttgccgacga
     4321 actcatggag tgccttgttg tccggatcga cggccagtgc gaaacgggca ttgctctcgg
     4381 tatattcatg cccgcagcac accaaggtat ccgcgggaag ggcggccagc ttgtgcaggc
     4441 tgtcaaacat ctctgctggc gtgccctcga tcagccggcc gcagccaagg ctgaacagcg
     4501 tgtcgccaga cagcagcacc gcgccgtcgg ggaagaagta gttgatctgg ccgcgcgtgt
     4561 ggccgggtgt gtcgatcacc cgggcgtcgg cgttgccgaa ccgtacggta tcaccctcct
     4621 tgaccgcttc atccagcttc ggtagccggt gctggtccgc caacgcgccc acaaccgggc
     4681 agttgaaccg agcgcgaacc tcatcgacgc cggcaatgtg gtcggcatgg tgatgggtca
     4741 acaggatcaa atccagccgg ccgccagcct tttcgattgc ttcgatgatc ggctgggcgt
     4801 ccgccggatc gacgatggcg gtcgcgccag tgctgtcttc gcgcagcagc caggcgtagt
     4861 tgtccttcag gatgggaacg ggggatgctg tgactgtcat gacaccttct ctagcactcg
     4921 ttcatacgat cgaaaaccac cgggcgtgtt atacagtggc gcgatgacag cggacgcaca
     4981 ctttgcggcg gaattctacg gttcggcacg tggcgctgtg gccgcccgca tcctgcggga
     5041 gcgactgctg gcgatgtggc cggacgccgc gggacagtcg gtgttgggta ttggatattc
     5101 gatgccgtat ctgcggcttt ggcgtgatcg tgcgaagcgc tgcatcgccc tgacgccggt
     5161 ccagatgggc gccgcgcgct ggccgcccgg attgccgaac ctgtcctgtg cggcggagga
     5221 ggacagcctg cccttcgctg acctgacgtt tgaccgcatc ctgctcgtgc acggtttgga
     5281 aatggcggaa aacggccgcc ggctgctgcg tgaggtctgg cgggtgctca aggacgacgg
     5341 aaggttgctg atcgtcgttc caaaccgggc cggcgtatgg gcctatagcg aaaatacccc
     5401 gttcggctac ggccagccat actcgacagg ccagctcggc agactcctcg ccagcggtct
     5461 gttccgcatc gagcggcgcg acgctgcttt gtggatgccg ccctcggggc tgcggatcgt
     5521 gctgcgcagc agtccgctgt tcgaacgcgg gggcaggaaa atggcgcccg ggctggcggg
     5581 cgtgaccctg acggaggcgg tcaaggacgt ttacgctgcg atgcctgttc gagccgcccc
     5641 gcgccgccgc ctggtgctcg cggaggcggc ctgacgaggc aggagcgtga tcacggtggg
     5701 tggaacgtcc aaagcgtaag aatcatgcga caaaagaaag agatagacca tttgcggcct
     5761 gcacagtcag gctgaaagtg acttagcgtg gccggatcag catgtagtgc atcgtgaaat
     5821 tgaactcgat cgggtcgctg atgccatgcg gcagtggcgg caggtgctgg tcgcgaaaga
     5881 gtgacaataa ggccaggtcg agccacatcg atcccgactt gccgatcagt tcgacctctt
     5941 taacccgccc gttcggcgcc gcaacgacgc gtacttttgc atccccctcc tgcaactccc
     6001 ggcgcgcttg ttcgggatag taggcgtgtt gggaaaccca ctggctcagc gcgttccgcc
     6061 agtccgcgcc cgccgcttcc tcgtcgcgct ccgacagcgg cgtaggatcg gctgtcccgc
     6121 gcgacgccgg ccccagcgac atgtcgatcg ttcctgggat atgcgggctg gaccgcgacg
     6181 aaggggatga tcgaggtgcg gggagcggcg tgccgaagct gaaattcgtc ggcggcggaa
     6241 attcggacga cttcggcgtg ggaactggcc tcggagccgg acgcatgggc ggaggtcttg
     6301 gcgccggcga cggtggctgc gggactggaa ccggactcgg ttcgggtggc gccggctgag
     6361 gtggcgccgg ctccggtatg ggcggactaa tcgggggcgg actgaccggg ggcggactta
     6421 atagcggcgg cgcgggcgtt gcgggaggtc gaacctccgg gggtgggggc ggcgaaaccg
     6481 gcttcgacgg tggctgttcg ggcggcggtg tcgcctgaag ccctggctcc ggcaacgtcg
     6541 ggccggtccg ccggccgctc tcgaacaaca tggtgaccgc cgagggcggc ggcagcagtt
     6601 cggactgcct ttcccgccgg atcgtgacca gcagcaaaac ggcgatcgcg acatggataa
     6661 gcagcgaaac caggatcgcg aggcgaaacc gccacggccg gtgacgaggg cgcaatcctg
     6721 ccagcgcgtc ggatcgccgc catcccctca gcggcatggc ccgaatccta tcggcgccgc
     6781 agcaggaaca cgccctgttc cccgaacagg ttggctaatc cagcggcgcg gcgccagggg
     6841 gcacggcggc catcctcgtc ggcggcgagc catcgctcca ccacgtagcc ttcttcctcg
     6901 cataacacga aaaaatcgcg gatcgtgcag ggatggatgt tcggtgtttc gtgccagggg
     6961 cggtcccagg tcgcggtcat cggcatccgg ccggtggaca gaagctgcca gcgcaccagc
     7021 caatggccaa aattcgggaa gctgaccacg gcacgcgtgc cgatccgcag catttcgcgc
     7081 agcacggcgc gtggccgttc aaccgcctgc aaggcacggg acagcacgac gtagtcgaat
     7141 gcgccatcgg ggtagtgcgc caggtcgaaa tcggcgtcgc catgcatcac cggcacgcca
     7201 tgcgccaccg cccgcgtgac ctccgccata tcgatctcga tgccgcgggc atcgcagttc
     7261 ttgtcctgaa aaaggtggtc gatcagcgtg ccgtcgccgc aaccgatgtc gagcacccgc
     7321 gaccccggcg cgatcattcc ggcgatcacc gccagatcga cccgcagatt cttggcgatg
     7381 aagcggaccg gatcgagcgt gctcatgatc cgatgtccag tcgggcatga accgcgcagc
     7441 cccgcaggaa cccggcgagg gtgcggtgaa aatccggctc gtccagcagg aatgcgtcgt
     7501 ggcccttgtc ggtcgggaac tccacaaacg acacgttcgc ccctgcccgg ttcagcgccc
     7561 gcgccacggc tcggctttcc gtcgtcggaa acagccagtc ggaggtgaac gacgccaaca
     7621 ggaagcgggt ccgcgtgccc ttgaaggctg tcgccaggtc accgccgcaa tcggcagcca
     7681 cgtcgaaata gtccatcgcc ctggtgatgg tcaggtagct gttggcgtcg aagcgctgca
     7741 cgaaggtgct gccctggtgg cgcaggtagc tctcgacctc gaacatctcg ccgaacaggc
     7801 tgatcgcctc ggtgctgtcc ttcggcgcgt tctgcagccg gcggccgaac ttccgggtca
     7861 gggcttcttc ggacagatag gtgatgtggg cgcacatccg agccaccgcg agaccgcggg
     7921 cggggatgcg gccgctgcgc cagtactggc cgttctgcca gtctggatcg gcgaaaatcg
     7981 cctggcggcc aacctcgtga aaggcgatgt tctgtgccga gtgataggac gcggacgcga
     8041 tcggcaaggc cgcgaaaacc gcttcggggt aggatgccgc ccattgcagc acctgcattc
     8101 cgcccatcga tccgccgatg acggcgaata atcgctggat cccgaggtga tcgatcaggc
     8161 gtttctgcgc ccgcaccatg tcgcggatcg tgaccggcgg gaaatcggtg ccccagggtt
     8221 cgtcgatttc gcttccgtca ggcagttcgc ggttgctgag cggtcctgtg ctacccatgc
     8281 agccgccgag gacgttgggg cagatgacga aaaagcggtc tgtgtccacc ggccgacccg
     8341 gcccgacgac cgcatcccac cagcccggct tgccggtgac cgggtgctcc tccgcgacat
     8401 actggtccaa cgtcagtgca tgacacacca ggatcgcgtt cgatcgatcg gcgttgagcg
     8461 tgccgtaggt gcgataggcg acggtatggc ggcgtagggt cgtgccacac tccagcgcga
     8521 tcccatcgtc gaacgtgacg tgggtatggc cggagaaaat agccgcatcg gcgtgtgtga
     8581 ctggctgatc catcgcgacg cttgcatagg acctgccggc cctgctcgca accagcttcg
     8641 ctatgcgccg cattcgcggt gtgatcgcgg cggaatgggt ttgcagaccg atgcgcttcc
     8701 gctaagtctg ccaccccctg acggcgagaa cgatgtccat cccggtattc acagttgacg
     8761 atattcaggc cagcggcgag gaggaacctg ctccccggga cggctggcga tccggtggtt
     8821 tgcccgcgct tcgggccgaa ctggatagaa tcgacaacgc ggtgcaagac ctgctgattc
     8881 agcgcgctga aatcgttgaa catgttgccc gttcgggcaa gccggccgca ttccgaccgg
     8941 gccgcgaagc ctccatcatc cggcgattgg tcggccgcca ccacgggtcc ctgccgccgg
     9001 tcacgttggt ccgcatctgg cgagagcttc tggccggcac gacgtcgatg caaggcggtt
     9061 tttcgctggc ggtttgcgac cccggtcacg gggcacctat gacgcagttg gcgcgcgagc
     9121 atttcggcgc actgacaccg ctgcgcgcgt acgcaagccc tgaccaggcg ttgatcgacg
     9181 tcaaccaggg cgtcgcatcg gtcgcggtgc tgccttaccc gtcggaagca gagagctggt
     9241 gggttgcgct gctgcaccgt gaaccgcggc tgcatatcat cggccgcctg ccgttctgga
     9301 aaccgcgtcc ggatggattt ccatccgtgc aggctgttgt cgtggcctcc accccggcgg
     9361 atgccagcgg cgaggatcgg accttcctcg ggttcgaatg cgacggcgat gtcagcaggt
     9421 cgcggctgtc gagcgaactg gccccgacag gattgaagtg ggcatcggtt gtttgggtcc
     9481 gtcagcaagg gtcgcccgtg gcccacattc ttgtggaggt ggaggggtat ctgtccccgg
     9541 acgatgttcg cttggcacgc ctggggccgg ttttgcgccg gccggtcgtg ctgggcagct
     9601 atgccgtgcc aaccgccgga ggcgctggat gacgagcccg tcccctcgcc cggaaatcct
     9661 gacgatccat ccctatgtgg ccggcgagtc ggaattgccg ggggccaacc gaacggtgaa
     9721 gctgtcgtca aacgagggtg cgttcggtgt gccgccctcc gcccgggcgg caatcgccaa
     9781 agcagccgcg gaatcctacc gttatccgga tggcggcgcc gaccggctgc gtgaggcgat
     9841 cggcaagcgg tggggcctgg atccgcggcg catcgtctgc ggcgccggat cggacgatct
     9901 gctgtaccag ttctgcctgt cctatggcgg tccagggcgc gacatcgtca tgtcggcgca
     9961 tgggttctcg atctatcaca ttgccgggac ctatgccggc agccgggtga tcaaggttcc
    10021 cgaacgcaac ctgaccaccg atctggacgc catgctggcg gcggtgtcgc cagcgacgcg
    10081 gctgatgttc atcgccaatc ccaacaatcc gaccgggtcg atggttccag ttgaggagat
    10141 agcgcgggtc cgcgccgcgc tgccgccgga
//
LOCUS       DASWQN010001304         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1578132, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001304 DASWQN010000000
VERSION     DASWQN010001304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578132"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VGC82_22135"
     CDS             complement(<1..348)
                     /locus_tag="VGC82_22135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0426031.1"
                     /translation="MSLVTVEDLTVSFGNRVVVDRVGFTLDRGETLALVGESGSGKSL
                     TALSLLQLLPPGAVCGGRVVVDGQSVIGAGDGLLRRLRGGVAGMVFQEPMTSLNPLKR
                     IGKQIAEAVRLHRR"
     gene            complement(345..1388)
                     /locus_tag="VGC82_22140"
     CDS             complement(345..1388)
                     /locus_tag="VGC82_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0426032.1"
                     /translation="MHVSPLTRRRLAIFRAHKRGYWSLWIFLALFVVTLFAEFLANDR
                     PLLIHFDGHWYVPVLRDYSEDHFGAGFMPTDADYTDPEVQEAINAQGWMLWPAVRYSY
                     NTLVKNLPTPAPSPPSRKNLLGTDDQARDVLARVIYGFRISVLFGFTLTAISSVVGIA
                     AGAIQGYRGGMVDLLFQRFIEIWSGMPQLYLLIILASIITPSFWILLVFLLLFSWMNL
                     TGVVRAEFLRGRSLEYVRAAKALGVSDVSVMWRHILPNAMVATLTFLPFTLSGSVTIL
                     AGLDFLGFGLPPGSPSLGELVSQGKNNLQAPWLGITAFVVLGGLLTLLVFIGEAVRDA
                     FDPRKTPRIGPLA"
     gene            complement(1388..>2081)
                     /locus_tag="VGC82_22145"
     CDS             complement(1388..>2081)
                     /locus_tag="VGC82_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0426033.1"
                     /translation="SISLGLWSTLLVYFVSIPLGIAKAVRDGSRFDVVSSAAVLVGYA
                     VPGFLFAILLVVLFAGGSFVQWFPLRGLHSAGSENWSAGARIVDYLWHMVLPTVALVV
                     GGFAGLTVLTKNCFLEEIRKQYVITARAKGASEQRILYRHVFRNAMLLIIAGFPSAFI
                     GILFSSALLVEIIFSLDGLGLLGFESAIRRDYPVMFGTLYMFTLLGLVMQIVGDLLYT
                     VVDPRIDFEARQ"
BASE COUNT          476 a          682 c          628 g          295 t
ORIGIN      
        1 ccgacgatgc agccgcacgg cctcggcgat ctgtttgcca atccgcttca gtggattgag
       61 gctggtcatc ggctcctgaa acaccatgcc ggcaaccccg ccgcgcaacc gacgcagcag
      121 gccgtcccct gccccgatca cgctttgccc atccaccacg acgcgaccgc cgcagaccgc
      181 gcccggcggc agcaactgca gcagcgacaa tgcggtcagc gacttgccgg agccgctctc
      241 gccgaccagc gccagcgttt cgccgcgatc cagcgtgaag ccgacgcggt ccaccaccac
      301 gcggttgcca aaggaaacgg tcagatcctc gacggtcacc aggctcatgc gagcgggcca
      361 atccgtggcg tcttgcgcgg atcgaatgcg tcgcgaaccg cctcgccgat gaacaccagc
      421 agggtgagca gtccgcccag caccacgaag gcggtgatcc ccagccacgg ggcctgcagg
      481 ttgttcttcc cttgtgacac cagttcgccc agcgacggag accccggcgg caggccgaag
      541 ccaaggaaat ccaggccagc caggatcgtg acggagccgg acagcgtgaa cggcaggaat
      601 gtcagcgtcg cgaccatcgc attgggcagg atatgccgcc acatgactga aacatcggac
      661 acgcccaggg ctttggcggc acggacatat tccaggctcc ggccacgcag gaattccgcc
      721 cgcaccacgc ctgtcagatt catccagctg aacaacagga gaaacaccag aaggatccag
      781 aagctgggcg tgatgatgct cgccaggatg atcagcagat aaagctgcgg catcccggac
      841 cagatctcga tgaagcgctg gaacagcaga tccaccatgc cgccgcgata cccttggatc
      901 gcaccggcgg caattccgac caccgaggaa atcgccgtca gggtgaagcc gaacagcacc
      961 gaaatacgaa acccatagat gacacgagcc aaaacatccc gcgcctggtc atcggttccc
     1021 agcaggttct tgcggctggg cggcgagggt gccggcgtcg gtagattctt caccagcgta
     1081 ttgtaggagt agcgaaccgc cggccaaagc atccagcctt gggcgttgat cgcctcctgc
     1141 acttcgggat cggtgtagtc cgcatcagtc ggcatgaagc cggcgccgaa gtggtcctcg
     1201 ctgtagtcgc gcagcaccgg cacataccag tggccatcga agtggatcag cagcggccgg
     1261 tcgttggcga gaaactccgc aaacagggtc accacgaaca acgccaggaa gatccacaac
     1321 gaccagtagc cgcgcttgtg ggcgcggaag atcgccagtc gccggcgcgt caacggcgag
     1381 acgtgcatta ttgccttgcc tcgaagtcga tacgcggatc gaccacggtg taaagcaggt
     1441 cgccgacgat ctgcatcacc agaccaagca gggtaaacat gtaaagggtg ccgaacatca
     1501 ccggatagtc gcggcggatg gcggattcaa accccagcaa gcccaggccg tcgagcgaaa
     1561 aaatgatctc caccagcaaa gcggaggaga acaggatgcc gatgaacgcc gacggaaacc
     1621 cggcgatgat cagcagcatc gcattgcgga acacatggcg gtacaggatg cgctgttcgc
     1681 tggcaccttt ggctcgggcg gtgatgacgt attgtttgcg gatttcctcc aggaagcagt
     1741 tcttggtcag caccgtcagc ccggcgaagc cacccaccac gagtgcgacg gtcggcagca
     1801 ccatgtgcca aaggtaatcc acgatgcggg cgccggcgct ccagttttcg ctgccggcgc
     1861 tgtgcagccc gcgcagcgga aaccactgca cgaagctgcc gccggcgaac agcaccacca
     1921 gcaggatggc gaacaggaaa cccggcaccg cgtagccgac cagcaccgcg gcactgctga
     1981 ccacgtcgaa acggctgcca tctcgcaccg ccttggcgat gccgagggga atcgaaacga
     2041 aatacaccag cagcgtggac cacagcccca acgagatcga g
//
LOCUS       DASWQN010001305         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1051447, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001305 DASWQN010000000
VERSION     DASWQN010001305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051447"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VGC82_22150"
     CDS             <1..495
                     /locus_tag="VGC82_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0426034.1"
                     /translation="EMTADVEEMDRMIGGYLAFARGEGTEQALPVNLSAILDEVAAGA
                     RRAGTRLELNVPADLTLKLRADAVRRAITNLVDNAGRHAGRVVLAAVAQGRSVVVTVE
                     DDGPGIPADKRENVFRPFESGSSGGTGLGLTIARDIVRAHGGEIMLDDSPLGGLRARI
                     RLPV"
     gene            complement(677..2323)
                     /locus_tag="VGC82_22155"
     CDS             complement(677..2323)
                     /locus_tag="VGC82_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEY0426035.1"
                     /translation="MDFSITLVNLAGSVALLLWGVHMVQTGVQRALGARLRGLLSVAL
                     RNRFKAFLAGIGVTAILQSSTATGLMVTGFAAGGLVELVPALAVMLGANVGTTLIVQI
                     LSVNVAEVAPALILIGVLMFRRTSASPRDFGRVLIGLGLLLMALRQFVGLLDPFEDAP
                     SLRLLLGAVSTQPVLDVILAAGLTWAAHSSVAVVLLTMSFAAKGVVPPHAAFALVLGA
                     NLGSAINPVLEGGAGDDPSARRLPIGNLINRGIGVAIGLALLPLIGPWLVTVDPDNAR
                     VVADFHTGFNLILALAFFPLLTPYANLLRRMLPARIDPADPSRPIYLDAAAIEVPMVA
                     LGAATREALRMADALESMLQGLRDALQRPDRRQLGELKRLDDVLDTLNTAIKSYMTAL
                     PSEAMTEQDHRRVTEILSFATNIEHAGDVVDKGLLGIVAKQVKRGLVFSEADAEPLRR
                     IVDRLMTNLRAAAALLVTGDERAARLLVEEKEIFRSLEAEATAAHFEQLREGRIGSVE
                     TSAMHLDALRDLKRVNAHLVAAAAYPVLESKGDLLPSRLR"
     gene            complement(2651..>2855)
                     /locus_tag="VGC82_22160"
     CDS             complement(2651..>2855)
                     /locus_tag="VGC82_22160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426036.1"
                     /translation="HERHGIANTFGHTFQGVGEDAGIVGHGLSDGLPSCLTTASMDLL
                     RMRGLPQRFKIHFCRWRGQNHAA"
BASE COUNT          499 a          908 c         1003 g          445 t
ORIGIN      
        1 gagatgaccg ccgacgtaga ggagatggat cgcatgatcg gtggctacct cgctttcgcc
       61 cgcggcgagg ggacggagca agcgctgccg gtcaacctgt cggcgatcct ggacgaggtc
      121 gcggccggtg ccaggcgcgc cgggacacgg cttgagctga acgttccggc cgacctgacg
      181 ctgaagctgc gggccgacgc ggtgcggcgg gcgatcacca atcttgtcga taatgccgga
      241 cgccatgccg ggcgtgtagt actggcggcg gtggctcagg gacgctccgt cgtggtgacg
      301 gtggaggacg acgggcccgg cattcccgcc gacaaacggg agaacgtgtt ccgcccgttc
      361 gaaagcggat cctccggcgg caccggcctg gggttgacga tcgcccgcga tatcgtccgc
      421 gcccatggcg gggaaatcat gctggacgac agtccgctgg gcggcctgcg cgcccggatc
      481 agattgccgg tgtgacacca gcccgccgaa gaccgacaca tccgcatcag actcccggcg
      541 ccatggtggc cgaagcgcca tcatccacgg ctttggtcat acgcgcggaa ggcgcggatg
      601 ccgaccctgc gtcggtgtgc ggaggtggtg ccgccagtgg gtcgatctca tcgcgggtcg
      661 gtgtaacagg actggactac cgcagccggc tcgggagcag atcgcctttg ctttccagca
      721 ccgggtaggc cgcggcggcg acaagatggg cgttcacccg cttcaggtcc cgcagcgcgt
      781 ccagatgcat cgcgctggtt tccacgctgc cgatgcgccc ttcgcgcaat tgttcgaaat
      841 gcgcggccgt cgcctcggct tccaagctgc ggaagatctc tttttcttcg accagcagcc
      901 gcgcggcgcg ttcgtcgccg gtcaccagca gtgcggcggc ggcgcgcagg ttggtcatca
      961 gccggtccac gatgcgccgc aacggttcag cgtccgcttc ggagaacacc aggccgcgct
     1021 tgacctgttt ggcgacgatg cccagcagac ctttgtccac gacgtcgccg gcgtgctcga
     1081 tgttggtggc gaaggacagg atctccgtga cgcgacggtg atcctgttcg gtcatcgcct
     1141 ccgacggcag cgcggtcatg taggacttga tggcggtgtt cagcgtgtcc agcacgtcgt
     1201 ccaggcgttt cagctcacca agctgccggc gatccggccg ttgcagggca tcgcgcagac
     1261 cttgcagcat gctctccaac gcgtccgcca tgcgcagggc ttcccgggtc gcggcaccga
     1321 gcgccaccat tggaacctcg atcgcggcgg catccaggta tatcgggcgg cttgggtcag
     1381 ccgggtcgat gcgggcgggc agcatgcggc gcagcagatt cgcgtacggc gtcagcaggg
     1441 gaaagaaggc cagtgccagg atcaggttga agccggtgtg gaaatccgcg acgacccggg
     1501 cattgtccgg atcgacggtc accagccacg gcccgatcag gggcagcagc gcgagaccga
     1561 tagcgacgcc gatgccccgg ttgatcaggt tgccgatggg caggcggcgg gcggacgggt
     1621 cgtcgccggc gccgccctcc agcaccgggt tgattgccga acccagattg gcacccagca
     1681 caagggcgaa cgcggcatgc ggcggaacca cgccctttgc cgcgaacgac atcgtcagca
     1741 gcaccaccgc gacgctggaa tgcgccgccc aggtcaggcc ggcggccagg atcacatcca
     1801 gcaccggctg ggtggagacg gcgccgagca gcaaccggag gctgggggcg tcctcgaacg
     1861 gatcgagcag gccgacgaac tggcgcagcg ccatcagcag caggccgagg ccgatcagca
     1921 cccggccgaa atcgcgcggg ctggcgctgg tgcggcggaa catcagcacc ccgatcagga
     1981 tcagggccgg ggcaacctcg gcgacgttga ccgacaggat ctgcacgatc agcgtcgtgc
     2041 cgacgttggc gccgagcatg accgcgaggg cgggcacgag ctcgaccagg ccgccggcgg
     2101 cgaaaccggt gaccatcagg ccggtcgcgg tgctgctctg caggatcgcg gtcacgccga
     2161 tgccggcgag gaaggccttg aaccggttgc gcagcgcgac actcagcagg ccgcgcagcc
     2221 tggcgccgag cgcgcgttgc acgccggttt gaaccatgtg gacaccccac agaagcaggg
     2281 cgaccgagcc ggcgagattc accagcgtga tggagaagtc cacgagcgcc ctccagcggt
     2341 cgaagcagcc ttgaacgccg atggtagcag caatgttgcc ttgggcagag gggatggaag
     2401 gtccggtgcg ggactgttgc ggttggaagg catttgccgg cattaccgcc gcggtgacgg
     2461 cgatcagcgg tgcggtaaca gctttggaga attatctgcc cccgctgatc agcagccgat
     2521 attcccgctt ggcgcgtgat caaattcctg ccgcgccggc gaaaggccag acgtcggctg
     2581 cgcgcggtgg cgatcctcgc ggttgactat ggaccgttcg ggcttgattg cgaggaggct
     2641 ggatactcat tcatgcggca tggttctgcc cccgccagcg acaaaagtgg atcttgaaac
     2701 gttggggtag accacgcatc cgcagcaggt ccatagaggc tgtcgtcaag caggatggca
     2761 acccatcgga gagaccatga ccgacgatgc cggcatcttc accgacaccc tggaacgtgt
     2821 ggccgaacgt gttggcgatc ccatgccgct cgtgt
//
LOCUS       DASWQN010001306         3555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_655749, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001306 DASWQN010000000
VERSION     DASWQN010001306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655749"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VGC82_22165"
     CDS             complement(<1..140)
                     /locus_tag="VGC82_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011047528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEY0426037.1"
                     /translation="MARVKFLCDADRCIECNACVTACKNENDVPWGINRRRVVTINDG
                     KPG"
     gene            complement(154..3120)
                     /locus_tag="VGC82_22170"
     CDS             complement(154..3120)
                     /locus_tag="VGC82_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEY0426038.1"
                     /translation="MLMKRRDGSAGRARLQGIAAGLASGVLNRRTFLRRSGLAAGAGA
                     ALGLMPLGSVRKAQAGPKIVGAPTEIKKNVCTHCSVGCTVIAEVQNGVWVGQEPAWDS
                     PINRGSHCAKGASVRELVHGDRRLKYPMKLVNGEWQRLSWDQAINEIGDKMLEIRAKS
                     GADSVYLLGSAKFSNEGGYLFRKFAAFWGTNSIDHQARICHSTTVAGVANTWGYGAMT
                     NSYNDMRNSKTVVFMGSNAAEAHPVSLQHILTGKEINRANVFVLDPRFTRTAAHATEY
                     VRFRSGTDIAVIWGMLHHIFNNGWEDKQFIAQRVYGMDQIRAEVAKWTPEEVERVTGI
                     PGEQLKKVAETFAKQRPSAFVWCMGGTQHSVGTANVRAYCNLLLATGNVGTFGGGANI
                     FRGHCNVQGATDIGLDIVTLPLYYGLVEGAWKHWARVWEVEYDYLQSRFDEVPAKAGR
                     PARTRKQNMELPGIPSTRWFDATLANPDDVDQRDSLKAMVIMGHGGNTVPRMTEMVKG
                     IEKLELLVVADPHPTTFAAISERKNGTYLLPACTQFETSGSRTASNRSLQWGEQIVKP
                     IFESKDDYEIIYRLSAKLGFAEPMFKNIKVEDNHPSSEDLLREINRGGFSTGYSGQSP
                     ERLKAHMKNQGKFDLVTLRAKAGEPDIGGDYYGLPWPCWGTPEIKHPGTHTLYNTNLH
                     AKDGGGTFRARFGVVYEEKQPDGSVKNVNLLAEGSYSKGSELTDGYPEFTYGVLKKLG
                     WDKELTEAELATINKIGGNNPDGVGWAVDLSGGIIRVTLEHGVMAYGNGKARAVAWNL
                     PDPVPVHREPIYTARPELVAKYPTRPDGRQFRMPNLGFAIQKAAVDKGLAKQFPIILT
                     SGRLVEYEGGGEETRSNRWLAELQQDMFVEVNTGDAAERGIKDGGWVWVFGPENGSKT
                     RVKALVTDRVGKGVAFMPFHFAGWFQGVDQRGKYPKGNDPIVLGESVNTLTSYGYDPV
                     TGMHEGKVTLCQIQSA"
     gene            complement(3134..3367)
                     /locus_tag="VGC82_22175"
     CDS             complement(3134..3367)
                     /locus_tag="VGC82_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426039.1"
                     /translation="MNKPSKSKTPAGEGKSQKAAGVDRRRFFMMGGSAVAAAAVVPLA
                     STEAEAEESQAERVKARYKETEHVKNYYRVNRY"
BASE COUNT          618 a         1131 c         1088 g          718 t
ORIGIN      
        1 ccaggtttgc cgtcattgat ggtgacgacg cgccggcggt tgatgcccca gggcacgtca
       61 ttctcgttct tgcaggcggt gacgcaggcg ttgcactcga tgcaacggtc ggcatcacag
      121 agaaacttga cgcgagccat atctattctc ccctcacgcc gattggatct ggcacagggt
      181 cactttgccc tcgtgcatgc cggtgaccgg atcgtagcca taggacgtca gcgtattcac
      241 gctttcgccg agcacgatcg gatcgttgcc cttcggatat ttgccgcgct ggtcgacgcc
      301 ctggaaccag ccggcgaagt ggaacggcat gaacgcgacg cccttgccga cccgatcggt
      361 gacgagcgcc ttgacgcgcg tcttcgagcc gttttcgggc ccgaacaccc agacccagcc
      421 gccgtccttg atgccgcgtt cggcggcatc acccgtattg acctcgacga acatgtcctg
      481 ctgcaactcg gcaagccagc ggttcgaccg ggtttcctcg ccgccgcctt cgtattccac
      541 caggcgtccc gaggtcagga tgatcgggaa ttgcttggca agccccttgt ccacggccgc
      601 cttctgaatc gcgaaaccga gattgggcat gcggaactgg cgcccgtccg gtcgcgtcgg
      661 atatttggca acgagttcag gacgcgcggt gtagatcggc tcgcgatgaa ccggcaccgg
      721 atccggcaaa ttccacgcca ccgcgcgggc cttgccgttg ccatacgcca tcacgccatg
      781 ttccagcgtg acgcgaatga tgccgcccga cagatcgacg gcccaaccga cgccgtcggg
      841 attgttgccg ccgatcttgt tgatcgtcgc cagttcggct tcggtgagtt ccttgtccca
      901 gccgagtttt ttcagcacgc cgtaggtaaa ctcgggatag ccgtcggtca gttccgaacc
      961 cttgctatag gaaccttcgg ccagcaaatt gacgttcttc accgagccgt ccggctgctt
     1021 ctcttcatag acgacgccga agcgggcgcg gaacgtgccg ccgccgtcct tggcgtgaag
     1081 gttggtgttg tagagggtat gcgttcccgg atgcttgatc tcaggcgtgc cccagcaggg
     1141 ccacggcaaa ccgtaatagt cgccgccgat gtcaggttcg ccggccttgg cgcgcaaggt
     1201 caccaggtcg aacttgccct ggttcttcat gtgcgctttc agccgctccg gcgactggcc
     1261 ggaatagccg gtcgagaacc cgccccggtt gatctcccgc agcaagtctt cggatgatgg
     1321 atggttgtcc tccaccttga tgttcttgaa catcggctcg gcaaaaccga gcttcgccga
     1381 aagccggtag atgatctcgt aatcgtcctt cgactcgaag atcggcttga cgatctgctc
     1441 accccattgc agcgaacggt tggaagcggt gcgcgagccc gaggtctcga actgggtgca
     1501 agccggcagc aaataggtac cgttcttgcg ttccgagatc gcggcgaagg tggtcggatg
     1561 cggatcggcg accacgagca gttcgagctt ctcgatgccc ttcaccattt cggtcatgcg
     1621 cggcacagtg ttgccgccat ggcccatgat gaccatggcc ttcaggctgt cgcgttgatc
     1681 gacatcatca ggattggcca gcgtcgcatc gaaccagcgg gtagacggga tgcccggcag
     1741 ctccatgttc tgcttgcggg tgcgggcggg acggcccgcc ttggccggca cttcgtcgaa
     1801 gcgcgactgc aggtaatcgt attcgacctc ccagacccgc gcccagtgct tccacgcgcc
     1861 ttcgaccaga ccgtaataga gaggcagcgt cacgatatcg agaccgatat cggtcgcacc
     1921 ctgcacgttg cagtggccgc ggaagatgtt ggcgccgccg ccgaaggtgc cgacattccc
     1981 ggtcgccagc agaagattgc aataggcgcg gacgttggcg gtgccgacgc tgtgctgggt
     2041 gccgcccatg caccagacaa aggccgatgg ccgctgtttg gcgaaggtct cggcgacctt
     2101 cttcaactgc tcaccgggaa tcccggtcac acgttcgacc tcttccggcg tccacttggc
     2161 gacttcggcg cggatctggt ccatgccgta gacgcgttgc gcgatgaatt gcttgtcttc
     2221 ccagccattg ttgaagatgt ggtgcagcat gccccagatc actgcgatgt cggtgccgct
     2281 gcggaaccgg acatattcag tggcgtgagc tgcggtgcgg gtgaaacgcg ggtcgagcac
     2341 aaacacgttg gcgcggttga tctccttgcc ggtcagaatg tgctgcagcg acaccgggtg
     2401 cgcctcggcg gcattcgatc ccatgaagac gacggtcttc gagttgcgca tgtcgttgta
     2461 ggaattggtc atcgcgccgt agccccaggt attggcgacg ccggcgacgg tggtcgaatg
     2521 gcagatgcgg gcctggtgat cgatcgagtt ggtgccccag aacgccgcga acttgcggaa
     2581 cagatagccg ccttcattgg agaattttgc ggagccgagc agatacacgg aatcggcgcc
     2641 ggacttggcg cggatttcga gcatcttgtc gccgatctcg ttgatcgcct gatcccacga
     2701 caagcgctgc cactcgccgt tgaccagctt catcggatac ttcaggcggc ggtcgccatg
     2761 cacgagttca cgaaccgacg cccccttggc gcaatgcgag ccgcgattga tcgggctgtc
     2821 ccaagccggc tcctgaccga cccagacgcc attctggact tccgcgatca cggtgcagcc
     2881 gaccgagcaa tgcgtgcaga cgttcttttt gatttccgtc ggtgccccga cgattttcgg
     2941 gccggcctgc gccttgcgca ccgagcccag cggcatcaaa cccagcgcgg cacccgctcc
     3001 tgccgccaga ccggaccggc gcaggaacgt gcggcggttg agaactccgg aggcgaggcc
     3061 ggcggcgata ccctgcaaac gcgccctgcc cgcggatcca tctcttcgct tcatcagcat
     3121 gtgcgtggtc ccatcaatag cggttgacgc gatagtagtt cttgacgtgc tcggtttcct
     3181 tgtagcgcgc cttgacgcgc tcggcctggg actcctccgc ttcagcctcc gtgctggcca
     3241 gcggcacgac ggcagccgca gcgaccgccg acccacccat catgaagaag cggcgccggt
     3301 ccacacctgc tgccttttgc gacttgcctt cgccggcggg cgtcttgctt ttggatggct
     3361 tgttcattgc gatctccttc gcggcagttg cggtagccct ggcgtcactc ggacagcgta
     3421 aaggcctcgg attccagttc cataaagaca cgaccgacac gtccgacggc gcggtagaag
     3481 ttggccgacc cggatatctc aaggtcggca aacatccgcg cagcccacgg cttgaggtgc
     3541 cgctcgaaga atcgc
//
LOCUS       DASWQN010001307         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1578285, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001307 DASWQN010000000
VERSION     DASWQN010001307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578285"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VGC82_22180"
     CDS             complement(<1..542)
                     /locus_tag="VGC82_22180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0426040.1"
                     /translation="MIDFYYWPTPNGWKVSIMLEETGLPYTVKPVNIGAGDQFKPEFL
                     AISPNARMPAIVDHDAPDGPLPIFESGPILIHLAERSGRFRPTTPRGRKECLEWLFWQ
                     VGNLGPMAGQLSHFVNYAKSVQEGDHQYAHKRYAGEYNRCLGVLEHRLEGREYILDAY
                     SIADIISWPWVLIARPLGQSL"
     gene            complement(539..1723)
                     /locus_tag="VGC82_22185"
     CDS             complement(539..1723)
                     /locus_tag="VGC82_22185"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0426041.1"
                     /translation="MPGPLDGYRIVDLTSMISGPLATMILADQGADVIKVENPDGGDH
                     TRAANNRRNGFSASFLNNNRNKRSVALDLKLPSALQAMLRLLGTADVFIQNFRPGVAD
                     RMGLGESAVRAVAPEIIYVSISGFGETGPYAQKPVYDPLIQSVSGLASIQAGSDTERP
                     RLVRTIVPDKLTAYNAAQAITAALLSRERTGKGQHVRLSMLDAIIAFLWSSDMGSQTF
                     VGQDIPQQEAASFIDLIYETATTPISAAVQTNREWTALTRALDKPEWLEDSRFKSPAL
                     RQRHINGRLVMTQEALLTRSAEEWLVRLTQHGVPCAPVLTRTAMLSNEQVVANGIVVE
                     TDHQEAGRLRQARSAARFSRTPASIRHGGPALGEHTAEVLAELGYSSADIQALDTKEH
                     AV"
BASE COUNT          390 a          683 c          825 g          425 t
ORIGIN      
        1 agcgactgac cgagcggcct ggcgatcagg acccagggcc aggagatgat gtcggcgatg
       61 gaataagcgt ccaggatgta ttcgcgcccc tccagacggt gttccagtac gcccaggcag
      121 cggttgtatt cccccgcata gcgcttgtgg gcatactggt ggtcgccctc ctgcacgctt
      181 ttcgcatagt tgacgaaatg gctgagttgc ccggccatcg ggccgaggtt gccgacctgc
      241 cagaacagcc actccaggca ttccttccgg cctctgggcg tcgtcggcct gaagcggccg
      301 gatctttccg cgaggtggat caggatgggg ccggactcga agatcggcag gggaccgtcc
      361 ggcgcgtcgt gatcgacgat cgcgggcatc cgggcgtttg gactgatcgc caggaattcc
      421 ggcttgaact ggtcgccggc gccgatgttg accggcttca cggtgtacgg aaggccggtc
      481 tcctccagca tgatggagac cttccagccg ttgggtgtcg gccagtagta gaaatcgatc
      541 ataccgcgtg ttccttcgta tcaagcgcct ggatgtcagc cgacgaataa ccgagttcgg
      601 ccagcacttc ggccgtatgc tcgcccagcg cgggaccgcc gtggcggatc gaggcagggg
      661 tgcgggagaa ccgggccgcc gaacgcgcct ggcgcaaccg gccggcttcc tggtggtctg
      721 tctcgacaac gatgccgttg gcgacgacct gttcgttcga cagcatcgcc gtccgcgtca
      781 gcaccggagc gcagggcacg ccgtgttggg tcagccgaac cagccattcc tcggccgaac
      841 gcgtaagcaa ggcctcctgc gtcatgacca agcgcccgtt gatgtggcgc tggcgcaggg
      901 ccggactctt gaaacgagag tcctccagcc actccggctt gtcgagcgct cgggtcagcg
      961 cggtccattc ccggttggtc tgcaccgcgg cgctgatcgg agtggtggcg gtctcgtaga
     1021 tcaggtcgat aaagctggcg gcttcctgct gcgggatgtc ctgcccgaca aaggtctggc
     1081 tgcccatgtc ggaggaccag aggaacgcga tgatcgcatc cagcatcgac aggcggacat
     1141 gctggccctt gccggtgcgc tcacgcgaaa gcagcgccgc ggtgatcgcc tgggcggcat
     1201 tataggcggt cagcttgtcc gggacgatgg tgcggaccag ccgcgggcgc tccgtgtcgg
     1261 aaccggcctg gatggaggcc aggcccgaga cggactggat cagcgggtcg taaaccggtt
     1321 tctgcgcgta tgggccggtt tcgccgaacc cgctgatcga cacgtagatg atttccggcg
     1381 ccaccgctcg gacggccgac tcgcccaggc ccatgcggtc ggcgacgccg ggtcggaagt
     1441 tctggataaa gacgtcggcg gtgccgagca ggcggagcat ggcttgcagg gccgagggca
     1501 gcttcaggtc cagggccacg gaccgcttgt tgcggttgtt gttcaggaag gaggcggaga
     1561 aaccgttgcg gcggttgttg gcggcccttg tatggtcgcc gccgtccggg ttttcgacct
     1621 tgatgacgtc cgcgccctgg tcggcgagga tcatggtcgc cagcgggccg gagatcatcg
     1681 aggtcagatc gacgatccgg tatccgtcca agggtccggg catggtgttt ccttcgggtg
     1741 ttcggcgcag gatatggggg tggcggcgga cggtgcaaat cggcggttgg atagggggca
     1801 tggtgcccgc tggttgtttg gatcgacgcg tcacgtatgc tcgcagcaac gctgtaggcg
     1861 gcggcttcca gtcgccgtca acgctggggt gaaagcaaca aacggaaggc gccggccctg
     1921 gcctgggcgg atgctggagg atgcatgatg gcgggtcacc caatgcggca cattgtcctg
     1981 gttttcgcta ccctgctcgc gggcgccgca gacgccaggg tggtcttggc ggaagacggt
     2041 cagcctcagt cggccgcccc ggcgaccggt ggtcgaactg gcaaggaacg tctcggcgac
     2101 aaagggtccg atgaacagcg catcgatgat tgcaaagtgc cgaaggcgcg acggagcagg
     2161 gatagaccag ctaattgcca gtccgttgtt cacccatcgc attatgttcc atgataaacg
     2221 gaggagcgag cccggcttgg gggcgatcgt ggtggaggcg tcgagagcgc acaggccgac
     2281 ggttggggcc ttcagcctca caccatcgat ctgtgggctc gaa
//
LOCUS       DASWQN010001308         3810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_786133, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001308 DASWQN010000000
VERSION     DASWQN010001308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786133"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1637
                     /locus_tag="VGC82_22190"
     CDS             342..1637
                     /locus_tag="VGC82_22190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0426042.1"
                     /translation="MDAATEASAMRKVYLRILPLTMLLYFLCYIDRINVSFAALTMNK
                     DIGLDAWTYGLSSSAFYLGYVLFEIPSNLIMDKVGARLWIARIMVTWGIASAATAAVV
                     GPTSFLVVRFLLGVGEAGLFPGMLLLFTYWFPDKHRGRIIGSFTLALPISVALGAPIS
                     TAILGMDGILGIKGWQWIYLIEALPTVILGFFVLLMMTDNPAAAKWLTVPERTWLTRT
                     LEVERRAVESGGKYSVWQGITNPKVLLLSVNYLGIVTASLGLLLFVPQIIKSLGASTM
                     GVGYATSLAYVCAAISMFGFGWLSDRIGDRRWLLCLTCMMATVGLVIAGAMMGTWWSL
                     AGMCLATAGFYGTKGPFWAMPTMLLTGSAAAGGLAFINSFGNIGGAVGPAVVGWLKDT
                     TGSYSGGLYGLAAFTAASAVIAAGALHIPRRVASVAPAE"
     gene            complement(1798..>3810)
                     /locus_tag="VGC82_22195"
     CDS             complement(1798..>3810)
                     /locus_tag="VGC82_22195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426043.1"
                     /translation="FDPESEQTYKQKMSANRPAGSDAMLLGEHTFELPDGACNVRIEA
                     LRTRTHFRVVLSHPDFTASAEGAWSFDNEELTQTKVVRTGDRDTIEAAVEQIIIELED
                     DGIDEAEFAGFMNGLEGDADEMPLIGDDPAEPPEATSLDRNRMKAIAKRIAKKPDADI
                     GIEDRAWLEQTPQILPAITDALIAAASAAKRDEALVLAYHQLLSLQLEFVRYRQDRGW
                     EWANDMLVAFQRRLIALGQEEIIPRDDWFMMGTALAQARVPVADEVQIALADAGFKPD
                     EVDGPPEEMMRTLRGFMDQMAQMVSSPFEVVDSLQSSGAMLPATLRSFMATEFALSPQ
                     PILRDATPLLLLDDDAPVREAAARALEQTAHPDTLSPSSLRRAIAVRNWIPASNRPTL
                     DAAIRKARLDGVEIGAWPAPTPDLEYYASSIDGSGAQSILAASRAGKKGLFVGLLLRY
                     GTGLVDGWVDLDLPRGKINKLLREAQMATPCTRVGKPLVDTLVQHAIGTAVEQGAVPP
                     PSLLEIAELIGGTEWKDRRLDVKAEADRMFEALDPADRTPNGVEAGFVRGLEWMTKDD
                     VFATWFEDGPQVQKALAKLPRSNKTEMVALVMTEILPAKRLQWAERFLMLAKLCEAAA
                     ESKQRAKARDLVLVAHALASDDPIGAIPIMSVIALQTVRATLVGGW"
BASE COUNT          612 a         1197 c         1236 g          765 t
ORIGIN      
        1 gcgatgcgcc ggatcggatt gcagatcgta cagcgatgcg atcgctcggg cgcccccggc
       61 cagaccgggg tgcgtggtcg cgtccgtcac gcgttcgccg cgcagccaga cctcggtctt
      121 gcgctggcgc aaaccggcca ggtactgcgc gcccgtgcgt gccggcatgg cattcctccc
      181 gccctgtgtg ttggcaaaga ctttccaggg cgactggcgg cgggtcaagg atgcaggcgc
      241 ataaatgcgg cgatcggcgt ccgccggagt gggtctgcac cttttcgcca caccgcgatt
      301 tgccttaaca tgccgaaagg aaacgccaag gaggccatcg aatggatgcc gcgaccgaag
      361 cctcggccat gcgcaaggtg tatttgcgca ttctgccgct gacgatgctg ctgtatttcc
      421 tctgctacat cgatcgcatc aacgtcagtt ttgcagcgct gacgatgaac aaggacatcg
      481 gtctggacgc gtggacctac gggctttcat ccagtgcgtt ttatctcggc tatgtattgt
      541 tcgagatccc gagcaacctg atcatggaca aggtcggggc ccgtctgtgg atcgcgcgga
      601 tcatggtgac ctggggcatc gcttcggccg ccaccgccgc ggttgtcggc ccaaccagct
      661 tcctggtcgt ccgttttctg ctgggagtgg gggaagcggg gctgttcccc ggcatgctgc
      721 tgctgttcac ctactggttt ccggacaagc accgtggtcg catcatcggg tctttcacgc
      781 tggcgctgcc gatctccgtc gcgctgggcg cgccgatctc gaccgccatt ctcggcatgg
      841 acggcatact ggggatcaag ggctggcagt ggatctacct gatcgaggcg ctgcccacgg
      901 tcatcctcgg cttcttcgtg ctgttgatga tgacggacaa tccggcggcg gccaaatggc
      961 tgaccgtgcc cgagagaacc tggctgacgc ggaccctgga agtcgaacgc cgtgccgtcg
     1021 aatcgggcgg caagtacagc gtgtggcagg ggataacaaa tccgaaggtg ctgctgctgt
     1081 cggtcaatta cctcggcatc gtcaccgcga gcctggggct gttgctgttc gtgccgcaga
     1141 tcatcaagtc gctgggcgcc tccaccatgg gtgtgggcta cgccacgagc ctggcctatg
     1201 tctgcgcggc gatctcgatg ttcgggttcg gctggctgtc cgaccggatt ggcgaccgcc
     1261 gctggctgtt gtgcctgacg tgcatgatgg cgacggtggg cctggtgatc gccggcgcca
     1321 tgatgggcac ctggtggtcg ttggccggaa tgtgcctggc gaccgctggg ttttatggca
     1381 ccaaaggccc gttctgggcg atgccgacaa tgctgctgac cggttcggcg gcggcgggag
     1441 gcctcgcgtt catcaactcg ttcggcaata tcggcggtgc cgtagggccg gcagtggttg
     1501 gctggttgaa ggacaccacc ggcagctatt cgggtggatt atacgggctg gccgcgttca
     1561 ccgccgccag cgccgtcatt gcggcggggg ctctgcatat tccacgccgg gtggcgtcgg
     1621 tggcgccagc cgaataatcg tcatggctgg gagagcagac cttatcggcc tgtttgtgca
     1681 ggccgcaaat ggcctacctc attgttttca cgctcgggac atccgcccaa agtgatcacg
     1741 ctgtagcgct cccggccacg attgcggaac gtaatgcggc ttccgctacg gctgggatta
     1801 ccacccgccc accagtgtgg cgcgaaccgt ctgcagcgca atcaccgaca tgatcgggat
     1861 ggcaccgatc ggatcatcgc tggccagcgc atgggccacc agaacgagat cccgagcctt
     1921 ggcacgttgc ttggattccg ccgccgcctc gcacaacttg gccaacatca ggaaccgttc
     1981 ggcccactgc aggcgcttgg cgggaaggat ttcggtcatc accagcgcga ccatctcggt
     2041 cttgttggag cgcggcagct tcgccagggc cttctgcacc tgcggcccat cttcgaacca
     2101 ggtggcgaag acgtcgtctt tcgtcatcca ctccagtccg cggacgaacc cggcttcgac
     2161 accgttcggc gtgcgatcgg ccgggtccaa cgcctcgaac atgcgatcgg cttcggcctt
     2221 gacatccagc cggcggtcct tccattcggt gccgccgata agctcggcaa tttccagcag
     2281 cgacggcggg ggtacggcgc cctgctccac ggccgtgcca atcgcgtgct gcaccagcgt
     2341 gtcgaccaac ggcttgccga cgcgggtgca aggcgtggcc atttgcgcct cccgcagcaa
     2401 tttgttgatt ttgccgcgcg gtagatccag gtccacccag ccgtccacca agccggtgcc
     2461 gtaccgcaac agcaggccga cgaacagccc cttctttccg gcgcggcttg ccgccaggat
     2521 actctgcgct cccgaaccgt cgatgctcga cgcatagtat tccaggtccg gtgtaggggc
     2581 gggccaagcg ccgatctcga ccccgtcaag ccgggccttg cggatcgctg cgtcgagggt
     2641 agggcggttc gacgcgggaa tccaattgcg gacggcaatt gctcttcgca acgaacttgg
     2701 cgacagcgtg tccgggtggg cggtttgctc cagggcacga gcggcggcct cgcgcaccgg
     2761 ggcgtcatcg tccagcagca gcaatggcgt ggcatcccgc aaaataggtt gtggcgagag
     2821 tgcgaactcg gttgccatga agctgcgcag cgtcgccggc aacatcgcgc cggagctctg
     2881 cagcgagtcg actacctcga acggtgaaga caccatctgc gccatctgat ccatgaagcc
     2941 gcgcagggtg cgcatcattt cttctggcgg cccatccacc tcatctggtt tgaagccggc
     3001 atcggccagc gcgatctgga cctcgtcggc gaccggcacg cgtgcctggg ccagtgcggt
     3061 gcccatcatg aaccagtcgt cccgagggat gatctcttcc tgaccgagcg cgatcagccg
     3121 ccgctggaag gcgacgagca tgtcgtttgc ccactcccag ccccggtcct gccggtagcg
     3181 gacgaattca agctgcaggg acagcagttg atggtaggcg agcaccaggg cttcatcgcg
     3241 tttggcggcg ctggccgcgg cgatcaaggc gtctgtgatg gccgggagaa tctgcggcgt
     3301 ctgttccagc catgcgcggt cttcgatgcc aatatcggcg tccggctttt tggcgatgcg
     3361 tttggcgatc gccttcatgc ggttgcggtc gagcgacgtc gcttccggtg gttcagccgg
     3421 gtcatcgccg atcagcggca tctcgtcggc atcgccttcc aggccgttca tgaatccggc
     3481 gaattcagcc tcgtcgatgc catcatcttc aagttcgata atgatctgct cgacggcggc
     3541 ctcgatggtg tcccggtcgc cggtgcggac aaccttggtc tgcgtgagtt cctcgttatc
     3601 gaagctccag gcaccttccg cgctggcggt gaaatccgga tgcgacagga cgacgcggaa
     3661 atgggtccgc gtccgcaggg cttcgatccg gacgttgcat gcgccatcgg gcaactcgaa
     3721 cgtgtgctcg ccgagcagca tcgcatcgga accggccggc cggttcgccg acattttctg
     3781 cttataggtc tgttcgcttt cggggtcgaa
//
LOCUS       DASWQN010001309         6575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1183171, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001309 DASWQN010000000
VERSION     DASWQN010001309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183171"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..857
                     /locus_tag="VGC82_22200"
     CDS             276..857
                     /locus_tag="VGC82_22200"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY0426044.1"
                     /translation="MARFYEQALDQSEVPVIVDCGANIGVASVWYAQRFPQARIIAVE
                     PEPENFRILMMNAAVYPNITPVQGGISDRQTRISLTNVGDAPWAWETRESDDSGEIAT
                     FTIPELLATVPRSRLMTVKIDIEGAEVELFRSNLEWSVWAPLIVFESHDRLFAWRGTF
                     HAIAAALTHQPRDYIQKGENTFAFAHHFPSGSG"
     gene            1060..2637
                     /locus_tag="VGC82_22205"
     CDS             1060..2637
                     /locus_tag="VGC82_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0426045.1"
                     /translation="MRRRTLMQAGLAAALARPALVRAQSATTLKFIPYADLALLDPAV
                     SAFVTRNHVMMVFDTLFAMDEAGVAQPQMLEGFTTEHDGLTWTLTLRDGLHFHDGTPV
                     LARDAVASIRRWQINDAFGQALAAATDELSAPADKVIKFRLKHRFHLLPDALAHPTNT
                     MAVIIPERLAETPADVRLTEIVGSGPFRYLPAERVAGARNVYARFADYVPRPQGSPSF
                     CAGPRVAYFDRVEWLTTPDPGTQIAALMAGEVDWVEQPLMDLVPSLRAKSSLKTEVVE
                     TKGLIGFLRFNHLFPPFDNPAIRQAVLRAVNQSAFMEAVVGGNAAFDARCGIFTPNTP
                     LANDAGMAALNSTHGIAEARRALEAAGYKGERIVYLVPTDVPRINAIAEVGADMLRKI
                     GMNVDEVATDWGTTVQRSVSRQPLDKGGWSMFAAFTGGYDMSSPATAQLLRGNGPRAY
                     NGWPELPRLEALRDAWLAAEDQATQLAIARQIQAQALIDVPYLPVGLYLQPTAYRADL
                     TDMRKGLVQFTGVRRVG"
     gene            complement(2733..3140)
                     /locus_tag="VGC82_22210"
     CDS             complement(2733..3140)
                     /locus_tag="VGC82_22210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426046.1"
                     /translation="MLAEITRRSRFTAEEFQVLATEEPIDVSGLHRRIRRMIEDAEDF
                     IGRLPSEAVGVVFLDRGRPVQPDVNALDRYQRNSGALGGVWPTSSSIGHAMLERYKGQ
                     EPGYLAQVTDRFSGRAVMMDEDFIIYALRCQFR"
     gene            complement(3130..3645)
                     /locus_tag="VGC82_22215"
     CDS             complement(3130..3645)
                     /locus_tag="VGC82_22215"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEY0426047.1"
                     /translation="MPITKFQSNVLRLLAAQRSPDSYIAGGIAINRSGPRFSEDIDIF
                     PDSADRQTDAVKADEAALAAAGYAIIWPPNQRTAKQHAGIEKDGKQMQLEWVTDSAFR
                     FFPTQPDELFGYVLHPVDLAANKASAAADRRVPRDIVDLVTIHESILPLGSIVAASVG
                     KFPGTTGGDAG"
     gene            complement(3632..3841)
                     /locus_tag="VGC82_22220"
     CDS             complement(3632..3841)
                     /locus_tag="VGC82_22220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426048.1"
                     /translation="MSDNRAGDLQALYRQAFRDFGSVALWNMRPTQKPTPADALAITA
                     ALRRHGSMDGRRLAERIETLCRAHH"
     gene            complement(3994..4581)
                     /locus_tag="VGC82_22225"
     CDS             complement(3994..4581)
                     /locus_tag="VGC82_22225"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0426049.1"
                     /translation="MMHTADVLKVFAEHRGDSIVVSGRGGRHWIEISDTALDVPLGDP
                     AMGGHAGFGLGLALAQPNRRVVLFDSEGDILMSLGQLVTIAEQAPANFYHFILDNEVY
                     ATTGGQPVPGAKQVDYAAIARASGYPVARSFADLGDFSHGLPGILASPGPAFVTLKVL
                     PEVENTPIGQRVRWRKRSADKVVSDLLGALGNDRP"
     gene            complement(4597..5115)
                     /locus_tag="VGC82_22230"
     CDS             complement(4597..5115)
                     /locus_tag="VGC82_22230"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0426050.1"
                     /translation="MNGQTTAALSPETVMRQMKQNGVTDVVWLPDSETNWLYLLMQAD
                     PDLRLVGVTREGHACSIAAGLFAGGRRPMMLIQNTGMMESGDSIRGWLMGLNVPVVLM
                     VGYRGYTRHGSNTDTAATYTERFLMAFNIPFFLIENDADSDRITKAFELADKTRQPVV
                     VLVGDEYHGFNR"
     gene            5235..6113
                     /locus_tag="VGC82_22235"
     CDS             5235..6113
                     /locus_tag="VGC82_22235"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0426051.1"
                     /translation="MATTTFKLGDMTVHRIIEMEFGLTPALEFLPKLTPQMLDESRSW
                     MRSPMALDEQDRLVLCFQAYIVRTGRHVILVDSCVGNDKDRPARPVWHMKTDNVFMQG
                     LAEVGLTVNDIDFVLCTHLHVDHVGWNTRLENGRWVPTFPKARYLFSKTELDFWLAEN
                     DKTTLPPIADSVIPIVEAKRHDLVTSDHALSDLVRLIPTPGHTIDHYAVTLGKTGKDA
                     VITGDLIHTPLQAKWPDLAMRIDYDPLQSSATRWKFLESVCDTNTQCWFAHFPSPSRG
                     YVKRSGDGFKCEYITA"
     gene            complement(6114..6404)
                     /locus_tag="VGC82_22240"
     CDS             complement(6114..6404)
                     /locus_tag="VGC82_22240"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY0426052.1"
                     /translation="MAKTYWIGTYRSISSPEKLAAYAQIAGPAITAGGGKFIVRGTPA
                     KTYEAGLNQRTVVIEFESLAQAVAAHDSPAYQEALAALGDGAERDIRLVEGV"
BASE COUNT         1228 a         2089 c         1983 g         1275 t
ORIGIN      
        1 ctcttgtccc gaaaaagccg aataggagac aagttatctt acagggattt tcacgagccg
       61 ttcgtcagtt cggcctacgc accacgactc tcgcctatag cgctcgagta ttctgccgat
      121 ttgttccagg gaatcaatgg agggtcgtaa cgctgtcgct gaagggtgtt gcgtacccga
      181 tctcgctgcg acccgcaacg agtgattggg acgttctgca acaggttttc gtcgccgagg
      241 agtacgattc cgcatcggaa gtacacgaaa ccagtttggc gcgattctac gaacaagcgc
      301 tcgaccagtc ggaagtcccg gtgatcgtcg attgcggggc gaatatcggc gtcgcctcgg
      361 tctggtatgc ccagcgcttt ccccaggcca ggattatcgc ggtcgagccc gaaccggaga
      421 attttcggat tctcatgatg aatgccgctg tttatccgaa catcacgcct gtgcaaggcg
      481 ggatctcgga ccggcagaca cggatttctc tcaccaatgt cggcgacgca ccctgggcgt
      541 gggaaaccag ggaaagcgac gattcagggg agatcgcgac cttcaccatt ccggagcttc
      601 tggcgaccgt gccccggtcc cggctgatga cggtcaagat cgacatcgag ggagccgaag
      661 ttgaactgtt ccgctccaac ctggagtggt cagtttgggc accactgatc gttttcgagt
      721 cgcacgaccg gctgttcgcc tggagaggaa cgtttcacgc catcgccgca gccctgacgc
      781 atcagcctcg tgactatatc cagaagggtg aaaacacctt cgccttcgcg catcattttc
      841 caagtgggtc aggctagggc ggtttctgct caccttggtt cgcggacgcc gctctaagcc
      901 catggtctgg gggcgtaatt caccttctgg tgatccgccc gcaagagtcg cgcctaacca
      961 tccccagtct ccgcggcacg gcgccatcat cgcggcgata ttcgccaacg ccatggcata
     1021 cgccttgcct cagatcggcc gaacaatcgg aggatctcga tgcgccgccg caccctgatg
     1081 caagcgggac ttgccgccgc tcttgcccgt cccgcgctgg ttcgtgcgca atcggcgacc
     1141 acgctgaaat tcattcccta tgccgacctg gcgctgctcg atcccgcggt cagtgccttc
     1201 gtgacgcgca atcatgtcat gatggtgttc gacacgctgt tcgccatgga tgaagccggt
     1261 gtcgcccagc cgcaaatgct agagggtttt accaccgagc acgacggcct gacatggacg
     1321 ctgacactgc gggacgggct gcattttcat gacggcacgc cggttctcgc tcgggacgcg
     1381 gtcgctagta tccggcgctg gcagatcaac gacgcctttg gccaggcact ggccgcggca
     1441 accgacgaac tgtccgcacc cgctgacaaa gtgatcaaat tccgcctcaa gcaccgcttt
     1501 cacctgctgc cggacgcact tgctcatccc acgaacacca tggccgtgat cattccggag
     1561 cgtctggcgg aaacgccggc cgatgtccgg ctgacggaga tcgtcggcag cggcccgttt
     1621 cgctacctgc ccgccgaacg ggtcgccggc gctcgcaacg tttacgcccg gttcgctgac
     1681 tacgtccctc gcccgcaagg cagccccagt ttctgcgccg ggccgcgcgt agcgtatttc
     1741 gaccgggtcg aatggctgac cacgccggac cccggcacgc agatcgccgc cctgatggcg
     1801 ggcgaagtgg attgggtgga acagccgctg atggacctcg tgccatccct gcgcgcgaaa
     1861 tccagcctaa aaaccgaggt ggtggaaacc aagggcctga tcgggttcct gcggttcaat
     1921 cacctgttcc caccgttcga caatccggcg atccgccagg cggtgttgcg ggcggtcaat
     1981 cagtcggctt tcatggaggc ggtggtgggc ggcaatgccg ctttcgacgc gcgttgcggc
     2041 atcttcaccc cgaacacccc gctcgcgaac gatgccggga tggcggcgct caacagcacc
     2101 cacgggatcg ccgaggccag gcgagcgctt gaagccgccg gatacaaggg cgagcggatc
     2161 gtctatctgg ttcccaccga cgtgccccga atcaatgcga ttgccgaggt cggtgccgat
     2221 atgctgcgca agatcggcat gaacgtggac gaggttgcca ccgactgggg cacgacagtg
     2281 caacgttcgg tcagccgcca gccgttggac aagggcggct ggagcatgtt cgccgcgttc
     2341 accggcggct acgacatgag cagcccggcg actgcccagt tactgcgggg gaacggaccg
     2401 cgggcctata acggctggcc agagctgccg cgactggagg ccctccgcga cgcgtggttg
     2461 gccgcggagg accaggccac gcaattggcc atcgcccggc agatccaggc gcaggcactg
     2521 atcgacgtgc cctatctgcc ggtgggcttg tacttgcagc ccacggccta ccgggcggat
     2581 ttgacggaca tgcgcaaggg tcttgtgcag tttaccggcg tgcgccgggt tgggtagccg
     2641 ccaccttgtt ttgccgataa tccgccgcga attacggctt caggcaaggc cccagcattc
     2701 acaggcaact tgctctaccg ccattcactg ctttaccgga actggcaccg gagggcgtag
     2761 atgatgaagt cttcgtccat catcacggca cgaccgctga acctgtcggt tacttgggcc
     2821 aagtaaccag gctcctgtcc cttgtaacgc tcaagcatag catgcccaat actcgacgat
     2881 gtgggccata cgccgcccaa tgctccggaa ttgcgctggt atcggtccaa tgcattcaca
     2941 tcgggctgca caggcctgcc gcgatccaga aaaaccacgc ctactgcctc gctcggaagc
     3001 ctgccgatga aatcctcggc gtcctctatc atgcggcgta ttctgcggtg caggccgctc
     3061 acatcgatcg gttcctcggt tgccaacacc tgaaattcct cggcggtaaa tcggctgcgc
     3121 cgcgtaattt cagccagcat ctcctccggt ggtgccggga aacttgccga ccgaagccgc
     3181 gacgatggac ccaagcggca ggatgctttc gtggatggtg accagatcga caatatcgcg
     3241 cggcacccgg cgatccgccg ccgccgatgc cttgttggca gcaagatcga ctgggtgcag
     3301 cacgtagccg aatagctcgt cgggctgcgt tgggaagaac cggaacgcgg agtccgttac
     3361 ccactcaagc tgcatctgtt tgccgtcttt ttcgattccc gcgtgctgtt tggccgtgcg
     3421 ctgattaggc ggccatatga tggcgtaacc ggcggcggcg agcgcagcct cgtccgcctt
     3481 gacggcatcg gtctgccggt cggctgaatc agggaatatg tcgatatctt cggaaaaacg
     3541 tgggcccgac cgattgatcg cgatgccgcc cgcgatgtag ctgtcggggc tgcgctgtgc
     3601 cgccagcagc cgaagcacat tcgattgaaa cttagtgatg ggcacggcaa agcgtttcga
     3661 tacgctcagc gagtcgccgc ccgtccatac tgccgtgtct gcgcaaggcg gccgtaattg
     3721 ccagtgcatc agcgggagtc ggcttctgag tcgggcgcat gttccacagg gcgacggagc
     3781 cgaagtcccg gaacgcctgc cggtagagcg cttgtagatc gcccgcgcgg ttgtcactca
     3841 tggcaaggtc cttcccgcac agtatagcga gacggcaagc aaaaagggag ggcccttcga
     3901 ggcgccgcgt ccagtcagat cgttcaacct ttctgcaaca tgctgctttc ggccgggtgc
     3961 taactttcgc cggcccagcc ccgactccat gagttatgga cgatcgttcc ccaacgcccc
     4021 aagcaggtcg gaaaccacct tgtccgccga ccgcttccgc caccgcactc gctggccgat
     4081 cggcgtattc tcgacctccg gcagcacttt cagcgtgacg aatgctggcc ctggcgaggc
     4141 caaaatccct ggcaacccgt ggctgaaatc ccccagatcg gcgaagctgc gagccaccgg
     4201 atagccgctg gcccgcgcga tggctgcata atcgacctgc ttcgcccctg gcaccggctg
     4261 accgccagtt gttgcgtaca cctcgttgtc caggatgaag tggtagaagt tcgccggcgc
     4321 ctgttcggcg atggtgacca actgtcccag actcatcaga atgtcaccct ccgaatcgaa
     4381 cagcaccacg cgccggttcg gttgcgccaa cgccaggccc agcccgaaac cggcatggcc
     4441 acccatcgcg ggatcaccca ggggcacgtc caaagccgtg tcgctgatct cgatccagtg
     4501 acggccaccg cggcccgaga ccacaatcga gtcgccccgg tgttcggcga agactttcag
     4561 tacgtcagct gtgtgcatca tcgtctcacc ctcctgctag cggttgaagc cgtgatactc
     4621 gtcgccgacc agcacgacga ccggttgccg cgtcttgtcg gccagttcga acgccttggt
     4681 tatgcggtcg gaatccgcgt cgttctcgat caggaagaac gggatgttga aggccatcag
     4741 gaaccgttcg gtataggtcg cggcggtgtc ggtgttgctg ccgtgccgcg tgtagccgcg
     4801 atagccgacc atcaggacca ccggcacgtt cagccccatc agccagccgc ggatcgaatc
     4861 accggactcc atcatccccg tgttctgaat cagcatcatc ggcctgcgcc ccccggcaaa
     4921 cagtccggcg gcgatcgagc aggcgtgccc ctcacgcgtc accccgacca gccgcaggtc
     4981 cggatcggcc tgcatcagca gatacagcca gttcgtctca ctgtcgggca gccagacgac
     5041 gtcggtaacg ccgttctgct tcatctgccg catcaccgtc tcgggactta atgccgccgt
     5101 ggtctggccg ttcatgcacc ttcccccatc acgtttccca ccgctttccc ccaagtttcc
     5161 ctggggctcg tttgtcgggc cgccgatacg cagctaacat cggatcggta gccagaacca
     5221 ggggagcacg cagaatggcg acaacaacat tcaagcttgg tgacatgacc gtccaccgga
     5281 ttatcgagat ggaattcggc ttgacccccg cgctggagtt cctgcccaag ctgacgccgc
     5341 agatgctgga cgaaagccgg tcatggatgc gaagcccgat ggcgctggac gagcaggacc
     5401 ggctggtgct gtgcttccag gcctatatcg tgcgcaccgg caggcacgtc attctggtcg
     5461 atagttgcgt cggaaacgac aaggaccgcc cggcccgccc ggtttggcac atgaagacgg
     5521 acaacgtgtt catgcagggc ctggccgagg tcggcctgac cgtaaacgac atcgacttcg
     5581 ttctttgcac gcatcttcac gtcgatcatg tcggctggaa cacgcggctc gaaaacgggc
     5641 gctgggtccc gacctttccg aaggcccggt atctgttctc gaagacggaa ctggatttct
     5701 ggctggccga gaacgacaag acgacgctgc cgccgatcgc cgacagcgtc attccgattg
     5761 tagaggcaaa acgtcatgat ctggtcacca gcgatcacgc gctcagcgat ctggtaaggc
     5821 tgattccgac ccccggtcac acgatcgacc attacgccgt gacgctgggg aaaaccggca
     5881 aggatgcggt gatcaccggc gacctgatcc acacgccgct acaggcgaag tggcccgatc
     5941 tggcgatgcg aatcgattat gatccactgc aaagctccgc cacgcggtgg aagttcctgg
     6001 agtcggtgtg cgacaccaat acgcagtgct ggtttgcgca tttcccgtcg ccgtcccgcg
     6061 gctacgtcaa gcggtcgggc gacgggttca agtgcgagta tattactgcg tagctacacg
     6121 ccttcgacca gcctgatgtc gcgttcggcg ccatcaccga gcgcggcaag ggcctcctga
     6181 taggctgggg agtcatgcgc ggcgacagcc tgcgccaggc tttcgaactc gatcacgacc
     6241 gtgcgctggt tgagacccgc ttcgtaggtc ttggccggtg tgccgcggac gatgaatttg
     6301 ccgccgcccg cggtaatggc gggtccagcg atttgcgcgt aggcggcaag cttttctggg
     6361 ctggagatgg agcggtaggt gccgatccag tatgtcttgg ccatgggttc ctccttgatt
     6421 ggaggttcgt ttaacacccg tcggcggccg gcgacaaatc gcggcatctg tgctacagct
     6481 acccgaatga gtgttgccct tcgtcagcca atgtcggtcg ctgaattcct ggcctgggaa
     6541 gaaaggcagg agttgcgatg ggagttcgat ggatt
//
LOCUS       DASWQN010001310         1717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1316977, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001310 DASWQN010000000
VERSION     DASWQN010001310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316977"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1625
                     /locus_tag="VGC82_22245"
     CDS             147..1625
                     /locus_tag="VGC82_22245"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0426053.1"
                     /translation="MLNPVSPLPLKDPTLFRQANCLDGKWVDADGGQTIVVKNPATGE
                     AIGEVPNMGVAETRRAIEAANRAYPGWRAMLAKERGAILRKLSDLMLANADDLAVIMT
                     AEQGKPLSESKGEIVYAASFIDWFADEAKRIYGDTIPQNAKGRRILVLKEPIGVFAAI
                     TPWNFPAAMITRKAGPGWAAGCTGVIRPASQTPFSALALAVLAERAGMPPGVCNVLTG
                     GSAAMGGELTSNPLVRKLSFTGSTEVGAKLLAQCAPTIKKTSMELGGNAPFIVFDDAD
                     LDAAVVGAMGSKFRNTGQTCVCANRILVQDGVYEAFSSKLKTAVEAMKVGNGMEPGVS
                     QGPLINAGALAKVEEHVANALSLGASVVTGGKKHRLGGNFYEPTVLANVPHDALIFRE
                     ETFGPVAPLFRFKTEEEAIRLANDTEFGLAAYFYSRDIGRIFRVGEALEYGIIGINEG
                     IISTAEAPFGGMKSSGLGREGSKYGIEDYLEIKYLAIGGIGT"
BASE COUNT          338 a          487 c          610 g          282 t
ORIGIN      
        1 gcgaaggaca ccttctgacc ggtggttgct gcttccgcgc cggcgacccc ggcgcctcag
       61 cccgtaaatc gacgagacct cctaccctga caccgccgct ggaagcccct atgttccgtg
      121 gcacaaggga taaggagaac ccagccatgc tgaacccagt atcgccgttg ccgctgaagg
      181 acccgacgct gttcaggcaa gcgaattgcc tggacggcaa gtgggtagat gcggatggcg
      241 gccagaccat cgtggtgaaa aacccggcga ccggggaagc gatcggcgaa gttccgaaca
      301 tgggcgtcgc ggaaacacgg cgggcgatcg aggcggcgaa ccgggcatat cccggttggc
      361 gggcgatgct ggcgaaggaa cgcggggcga tcctgcggaa actatcggac ctgatgctgg
      421 cgaatgccga cgacctggcg gtgatcatga ccgccgaaca gggcaagccg ctgagcgaaa
      481 gcaagggcga gatcgtctat gccgccagct tcatcgactg gttcgccgac gaggccaagc
      541 gcatctacgg cgacacgatt ccgcagaacg ccaagggccg ccgcatcctg gtgctgaagg
      601 agccgatcgg cgtgttcgcc gcaatcacgc catggaactt ccccgctgcg atgatcacgc
      661 gcaaggcggg gccgggctgg gcggcgggct gcacgggcgt gatacggccg gcaagccaga
      721 cgccgttctc ggccttggcg ctcgcggtgt tggcggagcg ggccgggatg ccgcccggcg
      781 tctgcaacgt gctgacgggc ggttcggctg cgatgggagg cgagctgacc tccaacccgc
      841 tggtccggaa actgtcgttc accgggtcca ccgaggtcgg ggcgaagctg ctggcgcagt
      901 gcgcgccgac aatcaagaag accagcatgg aactgggcgg caatgcgccg ttcatcgttt
      961 tcgacgatgc tgatctggac gccgcggtgg tgggcgcgat gggcagcaag ttccgcaaca
     1021 ccgggcagac ctgtgtttgc gccaaccgca tcctggtgca ggacggggtg tatgaggcgt
     1081 tctccagcaa gctcaagacg gcggtggaag caatgaaggt cggcaacggg atggagccag
     1141 gtgtcagcca aggcccgctg atcaatgccg gcgcgctggc aaaagtcgag gagcacgtgg
     1201 cgaacgccct gtcgctgggt gcgtcggtcg tgaccggtgg caagaagcac cgactcggcg
     1261 gtaatttcta cgagccgact gtgctggcga acgtgccgca tgatgcgctg atcttccgcg
     1321 aggaaacctt tggacccgtg gcaccgctgt tccggttcaa gaccgaggag gaggcgatcc
     1381 ggctggccaa cgacaccgag ttcgggttgg cggcctactt ctactcgcgc gatatcggcc
     1441 ggatattccg cgttggcgaa gcgctggagt atgggatcat cggcatcaac gaggggatca
     1501 tctcgactgc cgaggctccg ttcggcggga tgaagtcgtc aggccttggg cgggagggat
     1561 cgaagtatgg gatcgaggac tatctggaga tcaagtacct cgcgatcggc gggattggga
     1621 cctaaggcgg tttcacggcg accggaaaga cagttgtaga agtctagact taaactgaca
     1681 gttggggttt gatcggtggg acaaagatga ccggtgt
//
LOCUS       DASWQN010001311         2913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1052276, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001311 DASWQN010000000
VERSION     DASWQN010001311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052276"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VGC82_22250"
     CDS             complement(<1..306)
                     /locus_tag="VGC82_22250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY0426054.1"
                     /translation="MFTGIITALGRIRTIDPIGEGRDMRLVIDTPRAFLTDPPVALGA
                     SIACSGCCLTAVEFGADWFAVDASAETLAHTKLGTWTVGSRVNLERSLHVGDELGGHL
                     "
     gene            complement(288..1421)
                     /gene="ribD"
                     /locus_tag="VGC82_22255"
     CDS             complement(288..1421)
                     /gene="ribD"
                     /locus_tag="VGC82_22255"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY0426055.1"
                     /translation="MTSQDRDHMRAALVLARRGLGTTWPNPSVGCVIVHDGRVVGRGT
                     TAPGGRPHAEPIAVNMAGDQAKGATAYVTLEPCCHWGRSPPCTDALIAAGISRVVVAA
                     TDPDPRVNGQGLMQLRDAGIEVETGLLEAEALETLIGFNHRIRLGRPMVTLKLASTLD
                     GRIATHGGESQWITGTPARRLAHAMRGRHDAVMVGVGTVLADNPDLTCRIPGFRPPAS
                     NPTPNVRVIADSHLRTPLTSRLLASAGEIPTWFLLREGSNLARESAFAGIGAKLIKVP
                     GSSAGVDLAAAMTALGAAGLTRVLVEGGGQLAAALLRDDLVDRIAWFHAPSVMGGDGW
                     PAVQAFGVARLDQMPRFTRDSETIVGDDMLSEYTRQRSCSPAS"
     gene            complement(1632..2108)
                     /gene="nrdR"
                     /locus_tag="VGC82_22260"
     CDS             complement(1632..2108)
                     /gene="nrdR"
                     /locus_tag="VGC82_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY0426056.1"
                     /translation="MRCPFCGFDDTQVKDSRPTEDNGAIRRRRFCGNCGQRFTTIERV
                     QLRELTVIKSDGRRVPFDRDKLGRSIRISMRKRPIQEERIERIVNSVVRQLEASGDSD
                     IPSKQIGELVMDTLKELDKVAYVRFASVYRNFREAKDFEDFVGGLGPVAEGPVAEG"
     gene            complement(2115..>2913)
                     /locus_tag="VGC82_22265"
     CDS             complement(2115..>2913)
                     /locus_tag="VGC82_22265"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY0426057.1"
                     /translation="EHLARTEKPKMIIAGGSAYPRFIDFPRIRKVADEVGAWFMVDMA
                     HFAGLVAAELFPSPLPHAHVVTTTTHKTLRGPRGGMVLSNDLDLGKKINSAVFPGLQG
                     GPLMHVIAGKAAAFNEALQPEFRIYQKAVQDNARELAETLKQQGLDIVSGGTDSHLML
                     VDLRPKRVTGKAAEASLERAHMTANKNAIPFDPERPAITSGVRLGSPAGTTRGFGTTE
                     FHEIGLMINEVLEGLGRSNDGTNTAVEQAVGARVKELCSRFPLYPSR"
BASE COUNT          509 a          950 c          937 g          517 t
ORIGIN      
        1 cagatgacca cccagctcgt cgcccacatg cagcgatcgt tcgaggttga cgcgcgatcc
       61 gacggtccag gtgcccagct tggtatgggc cagcgtttcc gcggaggcat cgacagcgaa
      121 ccagtcggca ccgaattcaa ccgcggtcag gcaacagccg gagcaggcga tggacgcacc
      181 caatgcgacc ggcgggtcgg tcaggaatgc gcggggcgta tcgatgacga ggcgcatatc
      241 gcggccctcg ccgatcgggt caatggttcg aatccgaccg agggcggtta tgatgccggt
      301 gaacatgatc tctgccttgt gtactcgctc agcatatcgt cgccgacaat agtttcgcta
      361 tcccgcgtga aacggggcat ttggtcaagc ctcgcgaccc cgaacgcctg gaccgccggc
      421 cagccgtcac cccccatgac ggagggagca tggaaccagg cgatgcggtc caccaggtcg
      481 tcacgcagca gcgcggcagc gagttgaccc ccgccctcga ccaggacgcg cgtcagtccg
      541 gcggcgccaa gcgcggtcat tgccgcggcc agatcaacgc ccgcggacga tcctgggacc
      601 ttgatcagtt ttgcgccgat gcccgcgaaa gcgctttctc gggcgaggtt gctcccctcg
      661 cggagcagga accaggttgg aatttcgccg gcgctggcca gaagccggga cgtcagcggc
      721 gtgcgcaggt ggctatcggc gatcacccgg acattgggcg tgggattcga cgcggggggg
      781 cgaaaccccg ggatgcggca ggtcaggtcc ggattgtcag ccaggaccgt gccgacgcca
      841 accatcaccg cgtcatgccg tccgcgcatg gcatgcgcga ggcggcgtgc cggtgtgccg
      901 gtgatccact ggctttcacc gccatgcgtg gcgatgcggc cgtccagcgt ggaggccagc
      961 ttcagcgtca ccatcgggcg cccgagtctg atgcgatgat tgaagccgat cagcgtttcc
     1021 agcgcctcgg cttccagcag gccggtttca acttcgatgc cggcgtcgcg aagttgcatc
     1081 aggccttggc cgttgacccg ggggtccggg tcggtcgctg cgaccacaac gcggctgatt
     1141 ccggcggcga tcaacgcatc ggtgcacgga ggcgagcggc cccagtggca gcagggttcc
     1201 agcgtgacgt aggcggtggc gcctttcgcc tggtcgccgg ccatgttgac ggcaatcggt
     1261 tcggcgtgcg gccggccgcc gggtgccgtg gtaccccggc caaccacgcg gccatcgtga
     1321 acgatgacgc agccgacgga tgggttcggc caagtggtgc ccaggccacg acgggccaac
     1381 acaagggcag cgcgcatgtg atcccggtct tgggaagtca cgctcgaacg cgtccggggc
     1441 agcggtccat tacggcgtca cggctgtggc tggcacggtg accacctaca gtgtcacggc
     1501 catgtttggc acggcgaccc gctatggtgt cacggccgtg cttggcacgc ccatccagga
     1561 attcgcctga ccccaaaaaa gtcgtgaatg gccggcatcg ggccgagcgt ggcaccggag
     1621 gcacaggcca actatccctc tgccaccgga ccctctgcca ccggacccaa accgccgaca
     1681 aaatcttcga aatccttcgc ttccctgaag ttgcgataaa cactggcaaa acgcacatag
     1741 gcgactttat caagctcctt gagcgtgtcc atcaccagct caccgatctg tttgctgggg
     1801 atatcgctgt caccggacgc ctcgagctga cgcacgacgg aattgacgat ccgctcgatc
     1861 ctttcctcct ggatcggccg cttgcgcatc gagatccgga tcgaccgccc gagcttgtcg
     1921 cggtcgaagg gaacgcggcg tccgtccgat ttgatcaccg ttagttcgcg caactgcacc
     1981 cgctcgatcg tggtgaagcg ctggccgcaa ttgccgcaga aacgccgccg ccggatggct
     2041 ccgttatcct cggtcgggcg gctgtccttc acctgggtat cgtcaaagcc acagaacggg
     2101 cagcgcatcg cagcctaccg tgacggatac agcgggaagc gagagcatag ttccttaacc
     2161 cttgccccga ccgcctgctc caccgccgtg ttggtgccgt cattcgatcg acccagaccc
     2221 tccagaacct cgttgatcat caggccaatc tcgtggaatt ccgtagtgcc gaaaccgcgc
     2281 gtggtgccgg ccggcgagcc aagccgaaca cccgaggtga tcgccggcct ttccggatcg
     2341 aacgggatcg cgttcttgtt ggcggtcatg tgggcgcgtt caaggctggc ctcggctgcc
     2401 ttgccggtga cgcgcttggg gcggagatcg accagcatca gatggctgtc cgttccgccg
     2461 ctgacgatat ccaggccctg ctgcttcagc gtttccgcca gttcgcgggc attgtcctgc
     2521 accgccttct gatagatgcg gaattccggc tgtaacgcct cattgaatgc cgcggccttg
     2581 ccggcgatga cgtgcatcag cggaccgcct tgcagcccgg ggaagaccgc ggagttgatt
     2641 ttcttgccca ggtccaggtc gttgctcagg accatgccgc cgcgcgggcc gcgcagggtc
     2701 ttgtgcgtcg tcgtcgtcac gacatgggca tgcggcagcg ggctgggaaa caactcagcc
     2761 gcgaccaggc cagcaaaatg cgccatatcg accatgaacc aggcgccgac ttcgtccgcg
     2821 actttcctga ttcgcggaaa atcgatgaag cgcggatagg ccgatccgcc ggcgatgatc
     2881 attttcggct tttccgtccg agcgaggtgc tcg
//
LOCUS       DASWQN010001312         6639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_393684, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001312 DASWQN010000000
VERSION     DASWQN010001312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6639
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393684"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1925
                     /locus_tag="VGC82_22270"
     CDS             291..1925
                     /locus_tag="VGC82_22270"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0426058.1"
                     /translation="MSSPLDACRSKRSRTSCREIFTQLANPYYIRDQAGLTQTSGWAD
                     AWTSTPSAYAIAVLRKADVVAAVNFARDRNVRLVIKGGGGGHSYQGTSAAPDSLLIWT
                     RDMNAITLHQAFQGIGCEATQAPQPAVTMGPGTIWMHAYEAVTTKGGRYVQGGGCATV
                     GVAGLIQSGGFGSFSKNYGTAAAGLLEAEIVTADGVVRIANACTNPDLFWGIKGGGGG
                     SLGVLTRLTLRTRELPNFFGTATGAIQASSEAAFRRLIGQFVTFYADALFNRHWGESV
                     TFRPDNIMELHMVFQGLNQRQAEAVWRPFLSFVAASPHDFTLAPAPRMLAIPARKFWD
                     PTFLNAAAPGLLVTDNRPGASADNVFYAGDQHEAGQFISGYDSVWLPAALLDKDQQQR
                     LADALFASTRHRQMALHFNKGLAGAGADEVEAARDTAMNPAVLDAFALAICGATGPAA
                     FPDIPGHEPDIAAARDRAASVGRAMQEVLKLVPNGGSYLSESNFFNDRWQQAFWGQNY
                     DRLAAVKKKYDPSGLFFVHHGVGSEEWSGDGFTRLT"
     gene            complement(2021..2983)
                     /locus_tag="VGC82_22275"
     CDS             complement(2021..2983)
                     /locus_tag="VGC82_22275"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0426059.1"
                     /translation="MQYTTLGRTGLKVSVAGLGSGGFSRLGLGTGHSAEHAVAIIRQA
                     LDLGVNLIDTAAAYGTEDVVGAALHGVPRDSVVVCTKASKPSGDPAFSIEKVVGSLNA
                     SLKRLRLDYVDVFQLHAVTQAAYDQVRDMIVPALLRERERGKFRFLGITEAASADPQQ
                     EMLGRAARDPIWDTAMLAFHLMNQVARTQVLPHTIANRIGTLMMFAVRSIFARPAQLV
                     AAMRDLAAAGQVPSSLADSDDPLGFLVHPEGASSVTDAAYRYVRHEPGVDVVLFGTGD
                     ARHLRTNIESILKPKLPEADRARLQALFSHLRGVGLVAPPAHAT"
     gene            3033..3878
                     /locus_tag="VGC82_22280"
     CDS             3033..3878
                     /locus_tag="VGC82_22280"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY0426060.1"
                     /translation="MRRPLDIQGHRGARALFPENTLEGFLAASKLGVAAFELDVGMTA
                     DGVAVVSHDLALNPDIVRDGFGAWLAQPGPTIWSLTYAELSRYDVGRLRPGTRTEALF
                     PDQRPHDGARIPTLASVLAALPTARFTIEVKTDPTHSDWTAPADDLADATLAVIEQAG
                     AIDRVIVEAFHWRIQRRIRGKRPDVTLAWLTRPETILDAASWWDGAASGRSVPATVAA
                     EGGPIWAPDYTSLTEALVHEAHALGLIVLPWTVNQPADMRRLIGWGVDGLISDRPDLA
                     LAVSA"
     gene            complement(4009..4860)
                     /locus_tag="VGC82_22285"
     CDS             complement(4009..4860)
                     /locus_tag="VGC82_22285"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0426061.1"
                     /translation="MTLSHHTIYANGIKQHYVDAGEGAPVVLLHGFPETWYAWRKQIP
                     ELARHYRLIVPDLRGYGETEKPPSGYDKRTMAADIKALMTALDIERAPIIGHDRGARV
                     ATRFAKDYPEAITHLGVFDNVPTRVIFEKMNATIARGHWFFIFNQTPDLPEALIAGRE
                     EIWLRYILQGWTYDPEALTPEDLTAYVRAYQKPGAVRGAMSDYRAAPEDVAQDQQDAA
                     VKIDCPTLALWGEDFEIGGRMWDMAGIWHEMGTNVRTVAIPKCGHLPHEERPEQVNAA
                     ILDFLSD"
     gene            5351..6019
                     /locus_tag="VGC82_22290"
     CDS             5351..6019
                     /locus_tag="VGC82_22290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY0426062.1"
                     /translation="MCGRYASFLPPDLIARLFGTKNPLPNLKPTWNMAPTMDAPVVRR
                     HPESGERHLDALTWGFIPAFTKSLKEARRPVNARSETVATSGMFRNAFTKRRCIVPAA
                     AFYEWQAGPTGKMPYAIARADGQPLAFAGIWEGWRAPDGDVLRTFAILTTAANAEMSI
                     LHERMPVILESSDWPAWLGEQPGDPVTLLRSAPEGTLRLWAVDKRVGNVRNDGPELLE
                     PLAA"
BASE COUNT         1228 a         2086 c         2067 g         1258 t
ORIGIN      
        1 tcctcctacg ctgtcccgtt gttttgcccc atactggccg gtcaccaatc ggagcattcc
       61 cgttttgcgc ttaattaagt tgtcacggcg ttatagatca ggcaacgttt cggtatgaaa
      121 agacggaact tgctgcaggg cgcgggctct atcccgttcc tcccggtaga tttgccgcgc
      181 gctgctgcag ccgcgaatcc ggagcggcgc gttcgcccat ccgatcaggc atggcccgat
      241 catggcagtt gggacaagtt aaaccatgaa gtgggtggcg tcttgtaaag atgtcgtcgc
      301 cactcgatgc ttgcagaagt aagcgcagca ggacaagttg ccgcgaaatc ttcactcagt
      361 tggcgaatcc gtactacatt cgcgatcaag ccgggctaac ccaaacctcc gggtgggccg
      421 acgcatggac ctcgacgcct agcgcctacg cgattgccgt tctgcggaaa gcggacgtcg
      481 tggctgccgt caatttcgcc cgcgaccgaa acgtgcgtct ggttatcaag ggcggtggtg
      541 gcggacatag ctaccagggc acctctgccg caccagactc tctcctgata tggacgcggg
      601 atatgaacgc catcaccctg catcaggcgt tccagggcat cgggtgcgag gcaacacagg
      661 caccgcaacc cgcggtcacg atgggacctg gtacaatctg gatgcatgcc tacgaggcgg
      721 tgaccacaaa gggtggacgt tacgtgcagg gcggcggatg cgcgacggtc ggtgtcgccg
      781 gactgatcca aagcggcggc tttggaagtt tctcaaagaa ttatggaacg gccgcggcgg
      841 gattgctgga agccgagatc gtcaccgccg acggcgttgt gcggatcgcc aatgcttgca
      901 ccaatcccga cctgttctgg ggaatcaaag gcggcggcgg cggcagcctg ggcgtgctga
      961 cccggctcac gctgcgaacg cgtgagctgc ccaatttctt cggtacggca accggcgcga
     1021 tccaggcctc gtccgaggcc gcatttcgtc gcctgatcgg acagtttgtc accttctacg
     1081 ccgatgctct cttcaatcgc cattggggcg agagcgtcac cttccggccg gacaacatca
     1141 tggaattgca catggtgttc caagggttga accaacgcca ggccgaggcg gtctggcgcc
     1201 cattcctgag cttcgtggcg gcgtcgccgc atgatttcac cctcgcgccc gcacctcgga
     1261 tgctggcaat accggctcgg aaattctggg atccaacgtt tttgaatgcc gcggcaccag
     1321 gtcttctggt taccgataat cgccctgggg catccgctga caatgtgttt tacgccggcg
     1381 accagcacga agcgggacaa ttcatttctg gatacgattc ggtatggttg cccgctgcat
     1441 tgttggacaa ggatcagcaa caacgcctcg ccgatgcgtt gttcgccagc acacggcacc
     1501 ggcagatggc gttgcatttc aacaaaggtc tagcgggagc tggtgccgac gaggtggagg
     1561 ccgcgcggga tacggcaatg aatccagcgg tgctggacgc cttcgcactt gcgatttgcg
     1621 gcgccaccgg tcccgccgcg tttcccgata ttcctggcca tgaaccggat atagcagccg
     1681 cacgcgatcg cgccgcttcg gtcggccgag cgatgcagga ggtgctaaag ctggtcccga
     1741 acggcggctc gtacctgtcg gaaagcaact tcttcaacga cagatggcaa caggctttct
     1801 ggggccagaa ctatgacagg ctcgcggcgg taaagaagaa atacgatccg tccggcctgt
     1861 tcttcgttca tcacggcgtt ggttccgaag aatggagcgg cgatggcttc acgcgattga
     1921 cgtgaacctc aggccctcac gtcgcggctc gctttgttat gaaccgattg ccgtagtcgc
     1981 tgtcggcgca cgatcttgcg catcgcctgc aatccgtgat tcaggtcgcg tgagccggtg
     2041 gtgccaccag ccctacgccg cgcaggtggc tgaacagtgc ctgcagcctc gcccggtccg
     2101 cctccggcag cttcggcttc aggatcgact cgatgttcgt gcgcagatgg cgggcgtcgc
     2161 cggtcccgaa cagcacgaca tcgaccccgg gctcgtgccg cacgtaacga tacgcggcat
     2221 cggtcacgct gctggcgcct tcgggatgca ccagaaagcc caacggatcg tcgctgtctg
     2281 ccagcgatga tggaacctgg ccagccgccg cgagatcgcg catcgccgcg accaactgcg
     2341 ccggccgagc gaagatgctg cgcacggcga acatcatcag cgtgccgatc cggttggcga
     2401 tggtatgggg caatacctgt gttcgcgcga cctggttcat cagatgaaaa gccagcatcg
     2461 ccgtgtccca gatcgggtcc cgtgccgctc ggccgagcat ttcctgctgc ggatcggcgg
     2521 acgcggcttc ggtgatgccg aggaagcgga atttcccccg ttcccgctcc ctcagcaggg
     2581 ccggaacgat catgtcgcgc acttggtcgt aggccgcctg tgtgacggca tggagctgga
     2641 atacgtccac ataatccagc cgcagacgct tcagcgaagc gttcaggctg ccgaccacct
     2701 tctcaatgct gaacgccggg tcgccggagg gcttcgacgc tttcgtgcaa accaccacgc
     2761 tgtcccttgg cacgccgtgc agcgcagcgc cgaccacatc ctcggtgcca taggcggcag
     2821 cggtgtcaat caggtttacc cccaggtcca atgcctgccg gatgattgcg accgcatgtt
     2881 cggcggagtg gccggtgccg aggcccagcc ggctaaagcc gccgctgccg agtccggcga
     2941 cgctgacctt caggccggtg cggccaagcg tggtgtattg catcggcgag gtcccggact
     3001 atggtccgcc gaacgctagc agggcaggac ggatgaggcg acctctcgac atccagggcc
     3061 accggggtgc cagggcgctg ttcccggaaa acaccctgga aggctttctg gccgcatcaa
     3121 aacttggcgt cgcggcgttc gaactcgatg tcggcatgac cgccgatggg gtggcggtgg
     3181 tcagccacga cctcgcgctg aaccccgaca tcgtgcgtga tgggtttggc gcctggctcg
     3241 cccaacccgg cccaacgatt tggtcgctga cgtatgccga gctcagccgc tacgacgttg
     3301 gccgtctccg tcccggcacc cgcaccgagg cgctgtttcc ggatcaacgg ccgcatgacg
     3361 gtgcgcgcat tccaactttg gcttccgtgc ttgcggcact gccgacggcc cgcttcacca
     3421 tcgaggtcaa aaccgacccc acacactcgg actggaccgc ccccgccgac gatctggccg
     3481 atgccacgct ggcggttatc gagcaagccg gcgccatcga tcgggtcatc gtcgaagcct
     3541 tccactggcg gattcaacgc cgcatccgtg gcaaacgccc ggatgtcacg ctggcttggc
     3601 tgactcgacc ggagaccatt ctcgatgctg cctcgtggtg ggatggggcc gcctctggac
     3661 gatccgtgcc ggccaccgtc gcggccgaag gcggtccgat ctgggcgccg gactatacca
     3721 gcctgaccga agcactggtt cacgaagccc acgcacttgg tttgatcgtc ctgccgtgga
     3781 cggtgaacca gccggccgat atgcgccgcc tgatcggctg gggagtcgat ggcctgatct
     3841 ccgacaggcc ggatctcgcg ctggcggtgt ccgcctgatc agccttatcc agggcatgat
     3901 cgcgctgggc ggaacgtccg tcgtccgaag gacgcgctgt tcgcgcgaaa cgtgtgatca
     3961 ggcaaggaaa cagacaattt tggcctgcac agccaggcta aaaataatct agtcgctcag
     4021 gaaatccagg atcgcggcat tgacctgctc cggccgttcc tcgtgcggga ggtggccaca
     4081 tttcgggatg gcgacggtgc gaacgttggt gcccatttcg tgccaaatgc ccgccatgtc
     4141 ccacatcctg ccgccgattt cgaaatcctc gccccacaga gccaaggtgg gacaatcgat
     4201 tttaacggcg gcgtcttgct ggtcctgcgc cacatcctcc ggtgccgcac ggtagtcgct
     4261 catggcgccg cgcacggccc ctggcttctg gtaggcgcgg acataagccg tcaggtcctc
     4321 gggagtcagt gcctcgggat cgtaggtcca gccttgcagg atatagcgca gccagatttc
     4381 ctcgcggccg gcgatcagcg cctcgggcag gtccggcgtc tggttgaaga taaagaacca
     4441 atggccgcga gcgatcgtgg cgttcatctt ttcaaagatg acgcgggtcg ggacgttgtc
     4501 gaacacgcca agatgcgtga tcgcttcggg atagtccttg gcgaaacgag tcgccacgcg
     4561 agcccctcgg tcatgaccga tgattggtgc ccgctcgatg tcgagtgcgg tcatcagcgc
     4621 cttgatatcg gccgccatgg tgcgcttgtc atacccggac ggcggctttt cggtttcgcc
     4681 ataaccgcgc agatccggaa cgatcaggcg gtagtggcgg gccagttccg ggatttgctt
     4741 gcgccaggcg taccaggtct cggggaatcc gtgcagcagg acgaccggcg ccccttcacc
     4801 ggcatcgacg tagtgctgct tgatgccatt ggcgtagatc gtgtggtggg acagagtcat
     4861 gatcgttcgg tcgccgttct gctcgaaccc gccggcgcgg gcacgttgac ggcaatgttc
     4921 aatgtgaagc tgcgttcctc tcaggtgtct caagaccgcg cgagccagcg gtaacaccga
     4981 atcgagtgct gaccggtcat cggccgtccc atgcctgcac cacgctcgct tgcaacgcag
     5041 agcgcgccaa cgaccccgag gtcgcagaga atttccatgc acctcggcat gtcacgccga
     5101 tggatccttc acaccagccg catggcggcc caatggcaag gatcccgatc atcaacagca
     5161 aggaaggctt cctttctcct tggcctcggt gatcttcggg tcaaaagcct tggccagcct
     5221 tcgcacgccc cttgaagctg aacccgccga cggcacgatt ctccagcatc ggatcctaaa
     5281 tattccggtt cgtcgcggtc cgaccaccat ggggtttgac tgccgggacc gatcgcgcat
     5341 tctgtctcac atgtgtggac gctatgccag tttcctgcca cccgacctga tcgcccgcct
     5401 gttcggcacc aagaacccac tgccgaacct gaagccgacg tggaacatgg cgccgaccat
     5461 ggatgcaccc gtggtgcgtc gccatcccga atcgggcgaa cggcatctcg atgcgctgac
     5521 ctggggcttc atcccggcct tcacgaaatc gctgaaggaa gcccgccgcc cggtcaatgc
     5581 tcgttccgag acggtcgcca cgtccgggat gttccgcaac gccttcacca agcgccgttg
     5641 tatcgttccg gccgccgcat tctacgaatg gcaggcaggc ccgactggca agatgcccta
     5701 cgccatcgcc cgcgccgatg gccagccgct ggctttcgcc ggcatatggg aaggctggcg
     5761 cgcgcccgac ggcgacgtgc tgcgtacttt cgccatcctg acgaccgccg ccaatgccga
     5821 aatgtcgata ctgcacgagc gcatgccggt gatcctggaa tcatccgact ggccggcatg
     5881 gctgggcgaa caacccggcg atcccgttac tttgctgcgg tccgcaccag aaggcacgct
     5941 gcgcctgtgg gcggttgaca agcgggtcgg caatgttcgc aacgatggac cggagctgct
     6001 cgagccattg gcggcgtgac tttagagcag atcccgttca gatagattat ctgaacgggt
     6061 gaagatgctc gctaaaacaa tgagctaggc ggttttcagg taaaaggggc aaaaccccgg
     6121 gcacgcagat gcagcatgat gcaacctcaa cggaccgagt gacggaaatc cgccatcgtt
     6181 gtgctggtga aaagcgcaac aacgctcaag ataacataac ttgcccggga ttttgcccct
     6241 cttacgttgc cagtgagcct aggggaacgg aaacggtcta ataccaacgg ccgttgaccc
     6301 tgatttttgc gcgaccgtat ggacgggctc gcgacatgca tgtcccgggc atcttcgcta
     6361 cggcggccag tacgccgaga tggcctggac gcccgaggca ggcgcctggc catgacagcc
     6421 accatgggtc aaggtcaacg gccgctggta taagcgccgc ggggaacctt tagcttggaa
     6481 gccggctctg cgcgcgagcg tcaattcagg tcgggccgcg gtgcggcggt ggaggcagga
     6541 tgggaaatgg cgcatcatcc cggccattta acgatgacat gggaggacag gatgcgactg
     6601 cagggcaagg tggcactgct gaccggcgcg gcggcggcg
//
LOCUS       DASWQN010001313         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1052629, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001313 DASWQN010000000
VERSION     DASWQN010001313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052629"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="VGC82_22295"
     CDS             <1..1339
                     /locus_tag="VGC82_22295"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0426063.1"
                     /translation="LRPLPYRRYEFEDVVDAFRQLQASGHLGKIVLTPRRTAVSKRPI
                     ARPPPSFAVRPDRTYVLSGAVGGFGLRAALFLARHGARHIALLGRRGPNTPGAEAALL
                     QLRSAGAEARIFGCDVADAEHLGDTLDRIRSEMPPIVGVIHSAMVLDDSWAADLDADR
                     FAAVLRPKLGGAEALDRLTRADQVELFLLFSSITTLLGNPGQANYVAANAAMEAVAER
                     RHAEGLPALAVNWGPIADAGYLTRMERVSEALERLLGNAHMEADAALEALPALLATGL
                     PVCGLADVQWGMLTRQLPGLRAPLLAEMPLDTRTETSGRSMRLRLTALSPEEAAASVL
                     QVLVNEIATILRVDPVAVNADQAISGLGMDSLTSIELRTALEARLGIDIPLSALSSGA
                     TLRDMSARIARMAVAPTAPAASASPPSVLADDVVSRIRRFEDADDEEWIEAAQ"
     gene            1366..>2475
                     /locus_tag="VGC82_22300"
     CDS             1366..>2475
                     /locus_tag="VGC82_22300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0426064.1"
                     /translation="MTAATKARLLSRFAEQRHDADPAPEAGRARPPLGDAGVRADLRL
                     MHDAGALLGIESPFFRPHDGLAGAETRIAGQDLLNFSSYNYLGLNGHPVVAAAAKAAI
                     DRYGTSVSASRIVSGERRLHRELEASLAALYGTADCLVLVSGHATNVTVIGHLMAAGD
                     VVVHDALAHNSIVQGAVLSGAQRLSFPHNDIDALDRQLAGLRGRVKRMLVVVEGHYSM
                     DGDIPDLPRLVATVRRHGAYLMVDEAHSLGVLGAGGTGIAEHSGVDPNAVDIWMGTLS
                     KTLSGCGGYIAASQDIVDYLRCSAPGFVYSVGLSPPLAAASLAALGVMRAEPERVVRL
                     NANGQAFLSAAKSAGLNTGTSIGSAIVPVIVGSSIG"
BASE COUNT          367 a          811 c          856 g          441 t
ORIGIN      
        1 gttgcgacca ttgccgtacc gccgctacga gtttgaggat gtggtcgatg cattccgcca
       61 gctccaggca tccggccatc tcggcaaaat cgtgctgacc ccgcgccgga cggcggtctc
      121 gaagcggccc atcgccaggc cgccgccgag ctttgccgtg cgtcccgacc ggacctatgt
      181 gctgtccggc gcggtaggcg ggttcgggct cagggccgcg ttgttcctcg cccgccacgg
      241 cgcccgtcat atcgccttgc tcgggcggcg cgggccaaac acgccgggag ccgaggcggc
      301 actcctccaa ctgcgatccg ccggggccga ggcccgcatc ttcggctgtg acgtggccga
      361 cgccgagcac ctgggagaca cactggaccg catccgctcg gagatgccgc cgatcgtcgg
      421 ggtgatccac tccgccatgg tgctagacga ttcctgggcc gccgacctgg atgccgatcg
      481 cttcgccgcg gtcctgcgtc ccaagcttgg cggcgccgaa gcactggacc ggctgacccg
      541 cgccgatcag gtcgaactat tcctgctgtt ttcctcgatc accacgttgc tcggcaatcc
      601 tggtcaagcc aactatgtcg ccgccaatgc cgccatggaa gcggtcgcgg aacgccggca
      661 tgcggagggt ctgccggcgc tggccgtgaa ctgggggccg atcgccgacg cgggctacct
      721 gacccggatg gaacgggtga gcgaggcact ggaacgactg cttggcaacg cacacatgga
      781 agccgacgct gcgctggaag cactgccggc gctgctcgcc acaggtcttc ccgtctgcgg
      841 attggccgat gtgcaatggg gcatgctgac ccgccagttg cccgggctgc gcgcgccact
      901 gctggcggaa atgccgttgg ataccaggac ggagacctcc ggccggagca tgcgcctaag
      961 gctgaccgcg ctgtcgccgg aggaggccgc ggcctctgtg ctgcaggtgc tggtgaacga
     1021 aatagccacc attctccggg tcgatcccgt tgccgtgaat gccgatcagg cgatttcggg
     1081 attgggcatg gattcgctca cctcgatcga gctgcggact gcgctggaag cccggcttgg
     1141 catcgacatc ccgctatcgg ccttgtccag cggcgccacg ctgcgcgaca tgtccgctcg
     1201 tatcgcgcgg atggccgtgg ccccgactgc gccggccgcc tccgcttcgc cgcccagtgt
     1261 gctggccgac gatgtggtca gccgcatccg gcgcttcgag gatgccgacg acgaggaatg
     1321 gattgaggcc gcgcaatgac catcgggcgg gaatcgctgt ttggcatgac cgcggccacg
     1381 aaggcccggt tgctctcgcg gttcgccgaa caacggcacg atgccgatcc agccccggaa
     1441 gctggccgcg cgcgaccgcc gctgggcgat gccggagtgc gcgcggatct gcggctgatg
     1501 cacgacgccg gagcattgct gggaatcgag agcccgttct tccgcccgca tgacggtctg
     1561 gcgggggcgg agacgcgaat tgccggtcag gacctgctca acttctcttc ctacaattat
     1621 ctcggcctga atgggcatcc tgttgtcgcg gcggcggcta aggcggccat cgatcgctac
     1681 ggcacctcgg tttccgcgag tcgcatcgtg tccggcgagc ggcggctgca tcgcgagctg
     1741 gaggcatcac tggcggcgct gtatggcacg gcggactgcc tcgtactcgt gagtgggcac
     1801 gcgaccaatg tcacggtgat cgggcacctg atggctgccg gcgatgtcgt cgtgcatgac
     1861 gcgcttgctc acaacagcat cgtgcagggt gcggtgctgt ccggggcaca gcgtttgtca
     1921 tttccgcata acgatatcga tgcgttggat cgccaactgg ccgggctacg gggccgggtg
     1981 aagcggatgc tggtcgtggt cgaaggccat tacagcatgg atggcgacat tcccgacctg
     2041 ccgcggctgg tcgcgacggt tcggcggcat ggcgcctacc tgatggtgga tgaggcgcac
     2101 tcgcttgggg tgttgggcgc cggcggcacc ggcatcgccg aacactccgg cgtcgatccc
     2161 aacgcggtgg acatatggat gggcacgctc agcaagacgc tgtccggttg cggcggctac
     2221 atcgcggcga gccaggacat cgttgactac ctgcgctgtt cggcacccgg cttcgtctat
     2281 tcggtcggcc tgtcgccacc ccttgccgcc gcgtccctgg cggcgctggg tgtgatgcgt
     2341 gcggagccgg agcgggtggt gcggttgaac gccaatggcc aggcgttctt gtcggcggcg
     2401 aaatccgcag gcctcaacac cggcacgtcg atcgggtccg ccatcgtgcc ggtgatcgtc
     2461 ggcagttcca tcggt
//
LOCUS       DASWQN010001314         3608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_921692, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001314 DASWQN010000000
VERSION     DASWQN010001314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921692"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1620
                     /locus_tag="VGC82_22305"
     CDS             28..1620
                     /locus_tag="VGC82_22305"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0426065.1"
                     /translation="MVGAGFSGLYLLHRLRRKGFSTRCFERGDGVGGTWYWNRYPGAR
                     CDVESMQYSYGFDNELQQEWNWPEKFSGHADIRAYADHVADRFGLRAHIDFETEVCAA
                     HYDEGLRLWRIETSAGEKVSARHFVMAGGCISTAQIPKFEGLADYTGKTYHTGRWPHE
                     PVSFAGLRVAVIGTGSSAIQAIPVIAEEAAHLTVFQRQANFTIPSRNHPMTADYANSW
                     KSGYAEKRVEMRYMPNGVLRELNDKSALEASEEERLATYEERWRMGGSGFLGAFNDIL
                     TNREANDTMAEFVRDKIRQTVKDPVTAELLCPKTHPIGTKRLCVDSGYFETYNRDNVD
                     LVNISQTPIERLTPDGLMVNGRGFTFDAIVFATGFDAMTGTLFNVDIRGRAGLELKSK
                     WAAGPRTYLGLMTESFPNLFMITGPGSPSVKSNMITSIEQHVDLVADTIIHMRDQGLA
                     VIEPEHSAEDEWVDHVQEVAYKTLFPQANSWYMGTNIPGKPRLFMPYIAGVGRYRRIC
                     EEVVGEGYKGFRFEAEQALAAE"
     gene            1965..>3608
                     /locus_tag="VGC82_22310"
     CDS             1965..>3608
                     /locus_tag="VGC82_22310"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY0426066.1"
                     /translation="MTKSKPVLIDRHPGRSAQSIGLAHALGTDPDLIHYPSVGVVGTK
                     GDSQCYLGVMSKVDAIHASLRNRIGQAPGQLKLRLVQPEYTIATSDGIRNGTREMRYS
                     LIGREVTHDALCEHLEATGLAGTIAVVACDKPPVGTLAALLEHNQPAIIMSDGPIHPG
                     IDPNTGEPLDIVSAYQVAGNPDDAFRHHIACHACPGIGSCGGMFTYNTMQTFIGVVGM
                     QPLHMVAPASDDPRRVKEFADELVGYLADMMEKGLNPRDIVKRDAIRNAVIVAMAIGG
                     STNVTLHAPEIARAAGYADFWKDIMTPEEFNHLSRDVVPVLTDARPYGNYSMVDIDRV
                     GGVQVIVRELLEAGLLNGDMMTCTGETLSAQVARLGAKASDGKVIRTVEKPYKPTGGL
                     RVLGGNLSPDFSAILKLAGVEGGLENNTFRGIARVFEGEQGLLDALDRTPESFQNNDM
                     IIVRYEGPSGAPGMPEMLDPTSRITTLCRERGIVVGLMTDARFSGGSVGLVIGHVGPE
                     AALGGPIAFIENGDEIVVDLNTSTLNCTALNDPTTLVKRK"
BASE COUNT          726 a         1118 c         1115 g          649 t
ORIGIN      
        1 gccgtcggcg gtaccgtcga tgccgtcatc gttggagccg gtttctccgg tctttacctg
       61 ctgcaccgtc tgcgtaggaa gggcttctcc acacgctgtt tcgaacgcgg tgatggcgtg
      121 ggcggcacct ggtactggaa tcgctatccc ggcgcgcgtt gtgacgtgga aagcatgcag
      181 tattcctatg gcttcgataa cgagttgcag caggaatgga attggccgga gaagttctcc
      241 ggtcatgccg acatacgcgc ctatgccgat catgttgcgg accgctttgg cctgcgagct
      301 cacatcgatt ttgaaaccga agtgtgcgcc gcgcattacg acgagggtct gcgactgtgg
      361 agaatcgaaa ccagtgcggg agagaaggtc agcgccaggc attttgtgat ggccggtggc
      421 tgcatctcga ccgcgcagat ccccaaattc gagggcttgg cggactacac tggcaagacc
      481 tatcacaccg gccgctggcc gcatgagccg gttagcttcg ctggtttgcg ggtggcggtc
      541 atcggcacgg gctcctctgc catccaggcg attccggtga tcgccgaaga ggccgcgcat
      601 ctgaccgttt tccagcggca ggcgaatttc accatcccct cgcgcaatca tccgatgacc
      661 gcggactacg cgaactcatg gaaaagcggc tacgctgaaa agcgcgtgga aatgcgctac
      721 atgccgaacg gtgtattgcg tgagctcaac gacaaatcgg cgttggaggc gagcgaagag
      781 gagcggctcg ccacctacga ggagcgttgg aggatgggcg gctccggctt ccttggcgcg
      841 ttcaacgaca ttttgacgaa ccgcgaagcc aacgacacga tggcggagtt cgtccgcgac
      901 aagatccgcc agacggtgaa agatccggtg acggcggagt tactctgccc gaagacccat
      961 cccatcggaa ccaagcggct atgcgtcgat agcggttatt tcgagaccta taaccgtgac
     1021 aacgtcgact tggtgaacat cagccaaaca ccgatcgagc ggctgacgcc ggacgggctg
     1081 atggtgaacg ggcgcggttt cacatttgat gccatcgtct tcgcgactgg cttcgacgcc
     1141 atgactggca ccctgttcaa tgtggacatc cgtggccgcg ctggcctgga actgaagagc
     1201 aagtgggcgg cgggcccgcg cacctacctc ggcctgatga ccgagagttt cccgaatctg
     1261 ttcatgatca ccggtcccgg cagcccctcg gtgaaaagca atatgattac ctcgatcgag
     1321 cagcacgtgg acctggttgc cgacaccatc atccatatgc gcgatcaagg cctcgcggtc
     1381 atcgagccgg aacattcggc cgaggatgag tgggtcgatc atgtgcagga agtggcgtac
     1441 aaaacgttgt ttccgcaggc gaactcctgg tacatgggca ccaatattcc gggcaagccg
     1501 cggctgttca tgccctatat cgctggcgtc ggcagatacc gcaggatctg tgaagaagtg
     1561 gtgggggaag gctacaaagg ttttcgcttc gaggccgaac aggcattggc ggcggagtag
     1621 cttcgaagtc catcgcataa tcgcggacca gtggcggtgg acaacaaacg acgcttgtgg
     1681 gttctttagt cctcggttcg accccgtcaa ttgcccatgc agcgtgagac tccaacggag
     1741 aaaccccgcc cgcgcgccgc gcgaattgct tccggcgaaa gcacccaatg caccgccgac
     1801 taccacgtct tgggacgtcg atctggttag gcccgggcaa tcgctctaag ttcccggctt
     1861 gcaccgaaac cgcgattgca gcgatagacc tctcccgacg atcgggtgac cgcagcactc
     1921 cctgaagctc agcccaccgc acacgccacc ttaaggaagc cttcgtgaca aagtcaaaac
     1981 cggtccttat cgatcgccat cccggccgct cggcccagag catcggcctg gcgcacgcgc
     2041 tcggcaccga tcccgatctg atccattatc cttcggtcgg cgtggtcgga accaagggcg
     2101 acagccagtg ctatcttggc gttatgtcga aggtggacgc gatccacgca agcctccgga
     2161 accgcatcgg tcaggccccc ggtcaactca agctgcgtct cgtccagccc gagtatacga
     2221 tcgccacctc ggacggcatc cgcaacggca cgcgggaaat gcgctactcg ctgatcggcc
     2281 gcgaagtcac gcacgacgcg ctgtgcgagc atctggaagc caccggcctt gccggcacca
     2341 tcgcggtcgt ggcctgcgac aagcccccgg tcggcacgct cgccgcgctg ctggaacaca
     2401 accagcccgc gatcatcatg tcggacggcc cgattcatcc cggcatcgac cccaacaccg
     2461 gagagccgct ggacatcgtc agcgcctacc aagtggccgg aaacccggac gacgcctttc
     2521 gccatcacat cgcctgccac gcctgcccgg gcatcggaag ctgcggcggc atgttcacct
     2581 acaacacgat gcagacgttc atcggcgtcg tcggcatgca gccgctgcac atggtcgcgc
     2641 cggcgtccga cgatccgcgc cgggtcaaag aattcgccga cgagttggtc ggctacctcg
     2701 ccgacatgat ggaaaagggc ctgaacccgc gcgacatcgt caagcgtgac gctatccgca
     2761 acgcggtgat cgtggcgatg gcgatcggcg gttccaccaa tgtcacgctg cacgcacccg
     2821 aaatcgcgcg cgccgccggc tatgccgact tctggaagga catcatgacg ccggaggagt
     2881 tcaatcacct ctcgcgcgat gtcgtgccgg tgctgacgga cgcccgaccc tatggcaact
     2941 actcgatggt cgatatcgac cgggtcggcg gcgtgcaggt gatcgtgcgc gaactgctgg
     3001 aagcaggtct gctcaatggc gacatgatga cctgcacggg agagacgctc agcgcccagg
     3061 tcgcccgcct cggcgccaaa gcctccgacg gcaaggtgat ccgcacggtc gagaagccct
     3121 ataagccgac cggcggcctg cgcgtattgg gtggcaacct gtccccggat ttctcggcga
     3181 tcctgaaact agcgggcgtc gaaggcggcc ttgagaataa taccttccgc ggcatcgcgc
     3241 gggtgttcga gggtgagcag ggcctgctcg acgcgctcga ccggacgcca gaaagcttcc
     3301 agaacaacga catgatcatc gtccggtacg aggggcccag cggagcaccg ggcatgccgg
     3361 agatgctgga tccgacgtcg cggattacga cgttgtgtcg cgaacgcggc atcgtcgtcg
     3421 ggttgatgac ggatgcgcgc ttctccggcg gatcggttgg cctggtcatc gggcatgtcg
     3481 gaccggaagc cgcgctgggc ggaccgatcg cgttcattga gaacggcgat gagatcgtgg
     3541 tcgatttaaa cacgagcaca ctgaactgca ctgcgctgaa tgatcccacc acactggtaa
     3601 agcgcaag
//
LOCUS       DASWQN010001315         5540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1579607, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001315 DASWQN010000000
VERSION     DASWQN010001315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579607"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..594)
                     /locus_tag="VGC82_22315"
     CDS             complement(10..594)
                     /locus_tag="VGC82_22315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY0426067.1"
                     /translation="MDLKLLSWGLRPNRLPRLWLFTDSARLPNPRASIAALPRGKAGV
                     VLRHDGDRGRLALGWDVARICRTRRLMLVVAGDARLAARLHAGVHLRGGYWPGPLRPR
                     GLVSSSAHSVSDIRRARRAGASLVFLSPAFPTASHVDALALGPLRWAAMARRGGAGSA
                     IAALGGIDGRSVKRLPRDLCQAVGAIGALGTRSG"
     gene            complement(719..988)
                     /locus_tag="VGC82_22320"
     CDS             complement(719..988)
                     /locus_tag="VGC82_22320"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426068.1"
                     /translation="MAVSHPDVAEAAVIAALHPKWDERPLLLVVAAPGKTVDPASVLA
                     LYEGKVAKWWLPDDVVVVPELPHTATGKLLKTSLRTRFKDHYVKS"
     gene            complement(995..2422)
                     /locus_tag="VGC82_22325"
     CDS             complement(995..2422)
                     /locus_tag="VGC82_22325"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0426069.1"
                     /translation="MASVLRPTERSCSASDNPEVTPVLGLMQSQGLMISSILLHAARH
                     HGKGEIVSRTHENTTHRYSWAAAERRSRRLVRVLQKLGVGPQDRVGTLAWNDFRHLEV
                     YYAAPGMAAICHTINPRLHPDDVAYIINHAQDKVLFVDSSFAPLITAVAPKIAGTVKT
                     VVMLTDPVNMPDVTLAPGMTLACYDQLMDAADEDYVWPLFDENTASALCYTSGTTGRP
                     KGVLYSHRSTWLHAYATALPDVLCIRATSRVLPVVPMFHVNAWGMPYAAALTGASLVL
                     PGRHLDGASLANLLNAEQVSFTAGVPTVWLGLLQYLRTSGQRLETVERIMTGGSAAPP
                     LLIEAFRDEYGIAVEHGWGMTELSPVGTYNAPKSAQAGLTQDQAAAHMLKQGRILPGI
                     DMKIVDGQGQELPWDGVAFGDLMVKGPWIASAYYGDAADSALDKDGWFATGDVATIDA
                     DGFMEITDRSKDVIKSGGEWISSIT"
     gene            2487..3233
                     /locus_tag="VGC82_22330"
     CDS             2487..3233
                     /locus_tag="VGC82_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0426070.1"
                     /translation="MQQVMIVTGGSQGIGEAVARLAAARGYAVALTYQSNEALADTVV
                     ADIAASGGKAIAVRAEMADEASILSLYRRVDETLGPVTALVNNAGTPGTMGKIDTVTT
                     QTLDTVLAVNVRAPFLMIREAVSRMATDRGGAGGAIVNISSRAAELGGAGEWIHYAAS
                     KGALDSLTVGASKELAARGIRVNAVSPGLIQTDLHARAGLPDRLTRLVGGVPMGRVGS
                     TFEVATAVLWLLSAEASYITGVIVPISGGR"
     gene            3234..3926
                     /locus_tag="VGC82_22335"
     CDS             3234..3926
                     /locus_tag="VGC82_22335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0426071.1"
                     /translation="MDMKTALIVGVGPGLSASLARLFAKHGLQVAMASRDPDKLQKLA
                     EETAGVTFACEATEPEEVAALFDVVIATQGVPDVVVYNASARARGPVTDLIPEEVAHA
                     ISVSAYGGFLVAQQAARHMVARGSGAILLTGASASVKGYPNSSAFAMGKFALRGLAQS
                     MARELSPKGVHVAHFVIDGGIRSSVRPDPNDNPDSMLDPDAIAETYWYVANQPRSAWT
                     WEVELRPWVEKF"
     gene            complement(3958..5172)
                     /locus_tag="VGC82_22340"
     CDS             complement(3958..5172)
                     /locus_tag="VGC82_22340"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0426072.1"
                     /translation="MQKTLPTAARIRPKPLLDDPRASTATADLNLLLVSRNPAWFKAV
                     TGATRTLGHGDVLACGARDALARLAGSASHYSHLLVDRNDADGLLDELADLTAEVAAP
                     DTDMLVLGASDVRHPHIRSIATATPRSVLEALMTASPTRARPVMELAELRAALKGAMI
                     ETRYQPIVSITDRSPIGLEALVRLNHPEQGTLPPDLFVPQIEDAGLAAELTGLVSARA
                     FSDLAGPSLAGRGLRMSVNFPLDVLLMPAALDRLEEQRQAAGVAANRVIIELTESRPV
                     TDIPTLRRSLERLRMLGYGVAIDDVGPAVPRLAPLLELPFTSLKLDKILVQQVATSKA
                     ARSFLARTTANAKARGFTVVAEGIETEEIWDQMAALGVDEVQGFLVARPLPVAAVPVW
                     WDSWVGRNVPIP"
     gene            complement(5310..>5540)
                     /locus_tag="VGC82_22345"
     CDS             complement(5310..>5540)
                     /locus_tag="VGC82_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0426073.1"
                     /translation="ADQLLPEVMKIAERIANLSPVAVQFAKQAVNRAFESTLVEGVRH
                     ERALFLSLFGTPDQKEGMAAFVEKRKPNFQLS"
BASE COUNT         1011 a         1805 c         1798 g          926 t
ORIGIN      
        1 agcgactcgt cagcccgaac gagtgcccag cgccccaatc gctcccacgg cttggcaaag
       61 gtcccgcggc agtcgtttca ccgaccgacc gtcgatgcca ccgagcgctg cgatggctga
      121 gccggcaccg ccgcgacgcg ccatcgcggc ccagcgcagc ggtccaagtg ccagtgcatc
      181 gacatggctt gcggtgggaa acgccggcga caggaataca aggctcgcac ctgcccgccg
      241 cgcccgccgt atatcggaaa ccgagtgggc cgaactgctc accagtccgc gggggcgcaa
      301 cggacctggc cagtaaccgc cgcgaaggtg aacgccggca tgcagccggg ccgccagccg
      361 cgcgtcgccc gccacgacca gcatcagccg ccgggttcgg caaatgcgcg ccacatccca
      421 cccaagggcc aggcggccac gatcgccgtc gtgacgcagc accacgccag ctttgccgcg
      481 cggcagcgcg gcaatcgatg ctcgtgggtt gggaagccgg gcgctgtcgg tgaacaacca
      541 aaggcgcggc aaacggttcg gccgcagtcc ccagctcagg agcttcaggt ccatccggcg
      601 gcgcatccgt cacaggccgc caccggcatc aggttcgcgc gccaatcctt tgccatgccg
      661 atccttcgcc gtcaggccaa catggctcat cgcgagccgg cggcaggcca cacaccggct
      721 acgatttcac gtaatgatcc ttgaaccggg tcctcaggct tgtcttcagc aacttgcctg
      781 tcgcggtatg gggaagctcc ggtactacca ccacgtcatc cggcagccac cacttggcga
      841 ccttgccttc gtacagcgcc agcaccgacg ccggatcgac cgtcttgcca ggtgcggcaa
      901 ccaccagcag caacgggcgc tcgtcccatt tcggatgcag cgcggcgatc accgcggcct
      961 ccgccacgtc ggggtgggaa accgcaatat tttcctaagt aatggagctg atccactcgc
     1021 cgccggactt gatcacgtcc ttcgaccggt cggtgatctc catgaagcca tcggcatcga
     1081 tggtggcgac gtcaccggtg gcgaaccagc catccttatc cagcgcgctg tccgcggcgt
     1141 cgccgtaata ggcgctggcg atccacgggc ctttgaccat caggtcgccg aacgcgacgc
     1201 cgtcccacgg cagttcctgc ccctgaccat cgacgatctt catgtcgata cccggcagga
     1261 tgcggccctg cttcagcata tgcgccgccg cctggtcctg cgtcagtccc gcctgcgccg
     1321 actttggcgc gttgtaggtg ccgacgggac tgagttcggt catcccccag ccatgctcca
     1381 cagcgatgcc atattcgtcg cggaacgcct cgatcagcag cggcggcgca gcggaaccgc
     1441 cggtcatgat gcgctctacg gtttctaagc gttggccact agtacgcaga tattgcagca
     1501 atccaagcca aacggtggga accccggcgg taaagctcac ctgctcggcg ttcagcaggt
     1561 tcgcgaggct ggccccgtcc agatgccgcc cgggcagcac cagcgacgcg ccggtcagcg
     1621 cggcggcata cggcatgccc caggcattga catggaacat cggcaccacg ggcagcacac
     1681 ggctggtggc ccggatgcac agcacgtcgg gcaacgcggt cgcataggca tgcagccagg
     1741 tcgagcggtg gctgtacagc acgcccttcg gccgccccgt ggtgccggag gtgtaacaca
     1801 acgcgctggc ggtgttctcg tcgaacagcg gccagacata gtcctcgtcc gccgcgtcca
     1861 tcagctggtc gtagcaggcc agggtcatcc ccggcgccag cgtgacgtcc ggcatgttga
     1921 caggatcggt caacatgacg accgtcttta cggtcccggc gatcttcggc gcgacagcgg
     1981 tgatcagcgg cgcgaaactg ctgtccacga acagcacttt gtcctgcgcg tggttgatga
     2041 tgtaggcgac atcgtccgga tgcaggcggg gattgatggt gtggcagatc gccgccatcc
     2101 ccggggcggc ataatacacc tcgagatggc ggaagtcgtt ccatgccaag gtgccgaccc
     2161 gatcctgagg cccaactccg agcttttgca gaacgcgaac caggcggcga gacctgcgtt
     2221 cggctgctgc ccaggaatag cggtgcgtgg tgttctcatg ggtacgggat acgatctcgc
     2281 ccttcccatg atgccgcgcg gcatgcagga ggatcgagga aatcatcagt ccctgcgatt
     2341 gcatcagacc aagcacgggc gtcacctccg ggttgtcgct ggcggaacat gatcgttcgg
     2401 tgggtcgaag taccgaagcc atgttttgtt gttcttcact tctgatgata cgggaatgat
     2461 cggccaacaa aaggggggta ggcgccatgc aacaggtcat gatcgtaacc ggtggcagcc
     2521 aggggatcgg cgaggcggtg gccaggctgg cggcggcgcg gggatatgcg gtggcgctga
     2581 cctaccagtc gaacgaggca ttggccgaca cggtggtcgc cgacatcgcc gcatcaggcg
     2641 gcaaggccat cgccgtgcgc gccgagatgg ccgacgaggc ctcgatcctg tcgctttacc
     2701 gccgcgtgga tgaaacgctg ggtccggtta ccgcactggt caacaacgcc ggcacaccgg
     2761 ggacgatggg caagatcgac acggtgacga cgcagacgct ggacaccgtc ctggcggtca
     2821 atgtcagggc gccgttcctg atgatccgcg aggcggtgtc gcgcatggcg acagaccgcg
     2881 gcggcgccgg aggtgcgatt gtcaacatct cgtcacgcgc ggccgaactc ggcggcgccg
     2941 gtgaatggat ccactacgcc gcgtcaaaag gggcactgga cagcctgacg gttggggcgt
     3001 cgaaggaact ggcggcacgc ggcatccggg tgaacgccgt cagccccggc ttgatccaga
     3061 ccgacctgca cgcccgcgcg ggactgccgg atcgcctgac acggttggtt ggcggggttc
     3121 cgatgggtcg tgtcggcagc acctttgagg tggcgaccgc cgtcctatgg cttctttccg
     3181 ccgaagcatc ctatattacg ggcgtcatag tgccgatttc cggaggacgt tgaatggata
     3241 tgaaaaccgc actgatcgtc ggcgtcggcc ccggcctgag cgcctcgctc gctcggttgt
     3301 tcgcgaaaca cggattgcag gtggccatgg cgtcccgcga tcccgacaag ctgcagaaac
     3361 tggccgagga aaccgcgggc gtcaccttcg cctgcgaggc caccgaacct gaggaagtcg
     3421 cagcgctgtt cgacgtggtg atcgcaaccc agggcgtgcc tgacgtcgtc gtttacaatg
     3481 ccagcgcccg ggcacgcggc ccggtgacgg acctgatccc cgaggaggtc gcacatgcga
     3541 tctcggtcag cgcctatggt gggttcctgg tcgcgcagca ggccgcacgg cacatggtcg
     3601 cgcgcggttc cggagcgatc ctgctgaccg gggcgtcagc cagcgttaaa gggtatccca
     3661 actcgtcggc attcgcgatg gggaagttcg cgttgcgtgg gctggcgcag agcatggcac
     3721 gggagctctc gccaaagggc gtgcatgtcg cgcatttcgt gatcgatggc gggatacgga
     3781 gttccgtacg gccggacccg aacgacaacc cggacagcat gctggacccc gacgcgatcg
     3841 ccgaaaccta ttggtacgtc gcgaaccagc cgcggagcgc gtggacgtgg gaggtcgagt
     3901 tgcggccctg ggtggagaaa ttctgaagcg ccagtcatcg ctaccggcag ccgcgcatca
     3961 ggggatcggc acgtttctgc cgacccacga atcccaccac accgggacgg ctgccacggg
     4021 taacgggcgg gccaccagga agccttgcac ctcgtccaca cccagggcgg ccatctgatc
     4081 ccagatctcc tcggtttcta tgccctcggc aacgacggtg aagccccgag cctttgcgtt
     4141 ggcggtcgtc ctggccagaa agctgcgcgc tgccttggac gtggcaacct gctgcacgag
     4201 gatcttgtcc agcttcaggc tggtgaacgg cagttccagc agcggcgcca gtcgcggcac
     4261 ggccggaccg acatcgtcga tcgcgacgcc gtagcccagc atccgcaaac gctccagcga
     4321 ccggcgcaag gtcggaatat cggtgacggg gcgactttcc gtcagctcga tgatgacacg
     4381 gtttgccgcg acgccggcgg cttgccgctg ttcctccagc cggtccagcg cggccggcat
     4441 cagcagaacg tcgagcggaa aattgaccga catccgtagg ccgcggcccg ccagggaagg
     4501 ccccgcaagg tcgctgaacg cgcgtgcgga caccaggccg gtcaactcgg cggcgaggcc
     4561 ggcatcttcg atctgcggca cgaaaagatc cggcggaagc gttccctgtt ccgggtgatt
     4621 gaggcgaacc agggcctcta gcccgatcgg gctgcgatcg gtgatgctga cgatcggctg
     4681 atagcgggtc tcgatcatcg cacccttcag cgccgcacgc agttccgcga gctccatcac
     4741 cggccttgcc cgcgtcggag aagctgtcat caacgcctca agaacagacc gcggcgtcgc
     4801 agttgctatc gatcggatat gcgggtggcg gacatcggat gcacccagga cgagcatgtc
     4861 ggtatcgggt gccgccactt cggccgtcag atccgccaat tcgtcgagca gtccgtccgc
     4921 gtcgttgcgg tccaccagga gatgggaata gtgcgaggcc gaaccggcca ggcgggcgag
     4981 tgcgtcgcgg gcgccgcatg ccagtacgtc gccatgaccc agggtccggg ttgccccggt
     5041 tacggccttg aaccaagccg gattgcggct gaccaggaga agattgaggt cagcggtcgc
     5101 ggtcgaagcc cggggatcgt ccagcagggg tttgggtcga atgcgcgccg ccgtgggaag
     5161 ggttttctgc atgaccgctt ctaaccccat tcgacaggac gaggcgaggg aaatcccgat
     5221 agcggaatgt cacgcaagca aaatatccag acccgatcaa atatagtggc ttggtgccgg
     5281 ttccccgcca gcaacttcca agtgccgcgt tatgacaatt ggaagtttgg cttccgtttt
     5341 tcgacgaatg ccgccatgcc ttccttctgg tccggcgtcc cgaacagcga caagaacaac
     5401 gcgcgttcat gccgaacgcc ttccaccagg gttgattcga acgcccgatt aaccgcctgc
     5461 ttggcgaact gcacggccac cggcgacaga ttggcgatcc gttcggcgat tttcatcacc
     5521 tccggcaaca actgatcggc
//
LOCUS       DASWQN010001316         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2504200, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001316 DASWQN010000000
VERSION     DASWQN010001316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2504200"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VGC82_22350"
     CDS             <1..206
                     /locus_tag="VGC82_22350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine acetyltransferase"
                     /protein_id="HEY0426074.1"
                     /translation="IKARVLLMPGATDLYFRTADNEAELPFLTTASLRPIPSIWGHRA
                     GNPVANPADMQFIKTEVRAWLAR"
     gene            234..623
                     /locus_tag="VGC82_22355"
     CDS             234..623
                     /locus_tag="VGC82_22355"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0426075.1"
                     /translation="MAAEVTPSNPPAVRAPTGYTHAIQVTGDYRRLIISGQVGMALDG
                     SVPSTGEGQIAQALANLRAILTAHGMTVQNIVKTTAFLTDRGMLPAYRQARAAVFGEH
                     APASTLLFVAGLADPRWVVEIEAEAVA"
     gene            625..2124
                     /locus_tag="VGC82_22360"
     CDS             625..2124
                     /locus_tag="VGC82_22360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-CoA synthetase"
                     /protein_id="HEY0426076.1"
                     /translation="MDLLRRNTVGDAVRRAACLFRDRSALVFGDRDWSFNALDRAADR
                     VAGHFAGLGLHAGDRIAAYGRNSDAYFIAWLACVRGGFVHVPVNYALTGEELTYIVRQ
                     SGSGLVLTGAGLEGNLAGTDVASRSLDDLIAVALNDAAPSAAEPDLEDDSLAQIVYTS
                     GTTAAPKGAMMTHKAMVSQYYSVIHNMDYAETDRALAALPLYHTAQTHAFTMPQLLVG
                     ARTILIEAPAADVVLRLIEQERITSFFAPPTVWISLLRHQDFAVRDLSSLEKIYYGAS
                     IMPVPVLQELRERLPKARPYNCYGQTEIAPLATVLRPEEHDARPASCGRPCLNVQTRV
                     VDPDMNDVPPGTHGEIVHRSPHLMVGYWDKPAETAEAFAGGWFHSGDVGYFDEDGYLY
                     IVDRIRDVINTGGVLVASREVEEILFMHPAVSEVAVVGLPDDKWIEAVTAFVVLRPGH
                     DADATALLSLARQHLAPFKVPKKVVFVDNLPRNTAGKLLKRELRKQAAS"
BASE COUNT          404 a          682 c          692 g          404 t
ORIGIN      
        1 ccatcaaggc gcgcgtcctg ctgatgccgg gcgctacgga cctttacttc cgcaccgccg
       61 acaacgaggc ggaattgcca tttttgacaa ctgcctcgct gcggccgatt ccgagcatct
      121 ggggacatcg tgccggcaac ccggttgcca atccggccga tatgcagttc atcaagactg
      181 aagtgcgggc atggcttgca cggtaatcac gccaacaacg agggagacat cacatggccg
      241 ccgaagtaac tcctagcaac ccgcccgcgg tgcgtgcgcc gaccggctac acccatgcga
      301 tccaggtcac cggggattac cgccgactga tcatatccgg tcaggtcggc atggcgctgg
      361 atggcagcgt gccgagtacc ggcgaaggcc agatcgctca ggcgctggcc aatctacggg
      421 cgatcctgac ggcgcacggg atgactgtgc aaaacatcgt caagaccacc gcctttctta
      481 ccgaccgcgg catgttgccg gcctaccgcc aggctcgagc agcggtcttc ggcgagcacg
      541 cgccggcttc gacgttgctg tttgtcgccg gactggcgga cccgcgttgg gtggtggaaa
      601 ttgaagctga agccgtcgcc tgacatggac ctgcttcgtc gcaacacggt cggcgacgca
      661 gtgcgccgag ccgcctgttt gttccgcgac aggtcggctt tggtgttcgg ggaccgagac
      721 tggtcgttca acgcgttgga tcgggccgct gaccgcgttg ccggacattt cgccggtctc
      781 gggttgcacg ccggcgaccg gatcgccgcc tatggccgga actccgacgc gtatttcatc
      841 gcatggcttg cctgcgtacg cggcgggttc gtccatgtgc cggtgaacta tgcccttacc
      901 ggagaggaat taacctacat cgtgcgccag tcgggatccg ggctcgtgct gaccggtgct
      961 ggactggaag gaaacctcgc ggggacggat gtcgcctcgc gcagcctgga cgatctgatc
     1021 gctgtggctt tgaacgacgc tgcgccaagc gcggcggaac cagacctgga ggacgattct
     1081 cttgcacaga tcgtctatac gtccggcacg acggcggcgc cgaagggtgc aatgatgacg
     1141 cacaaggcga tggtgtcgca gtattactcg gtcatccaca acatggacta cgccgagacc
     1201 gaccgggcgc tggcggcact tccgctttat cacaccgcgc agacgcatgc tttcaccatg
     1261 ccgcagctcc tggtcggcgc acgcaccatc ctgatcgaag cgccggcggc ggatgtcgta
     1321 ctgcgcctga tcgagcagga gcggatcacc tctttcttcg cacccccgac ggtctggatc
     1381 agcctgctac gtcaccagga tttcgccgtg cgcgatttgt cgtcgctgga aaaaatttat
     1441 tatggcgcgt cgatcatgcc ggtgccggtg ctgcaggagc tgcgggaacg actgccgaaa
     1501 gcccgtccat acaactgtta tggccagacc gagatcgcgc cactcgcgac cgtgctgcgc
     1561 cccgaggaac acgatgcgcg tccggcctcc tgcggacggc catgcctgaa cgtgcagacc
     1621 cgcgtcgtcg atccggacat gaacgatgtg ccgccgggga cacatgggga aatcgtgcac
     1681 cgctccccgc atctgatggt gggctactgg gacaagccgg ccgagaccgc cgaggcgttc
     1741 gccggcggtt ggtttcattc cggcgacgtg gggtatttcg acgaggacgg gtacctctat
     1801 atcgtcgatc gcatccgcga cgtgatcaac accggcggtg tcctggtggc gagtcgggaa
     1861 gtcgaggaaa ttctgttcat gcaccccgca gtctccgaag tcgccgtggt gggcttgccc
     1921 gacgacaagt ggatcgaggc ggtaaccgct ttcgtcgtcc tgcgaccagg tcacgatgcc
     1981 gatgcaacag cgctactgag cctcgctcgg cagcatctgg cgcccttcaa ggtgccgaag
     2041 aaggtcgtgt tcgtggacaa ccttccgcgc aataccgcag gtaaacttct gaagcgggaa
     2101 ctcaggaagc aggctgcatc ataaagtcgc gcacgatcgg caccgcgtcc cgcttggggg
     2161 aaagcagaag ctggaagacc at
//
LOCUS       DASWQN010001317         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2504212, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001317 DASWQN010000000
VERSION     DASWQN010001317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2504212"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1736
                     /locus_tag="VGC82_22365"
     CDS             210..1736
                     /locus_tag="VGC82_22365"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0426077.1"
                     /translation="MAIAGSVAAPRRLTRAVAAPGSPIGSPYSHATELTTALTNKTVS
                     SRELVDDAIARIETLDTKVNAVVVRDFAAAREAAKAADEALARGERRPLLGLPMTVKE
                     SFNVAGLPTTWGNPKSRNWRPDADALAVSRLKAAGAVLLGKTNVPIDLADWQSYNEIY
                     GTTNNPWDLTRTPGGSSGGSAAALAAGFVALELGSDIGGSLRAPAHFCGVFAHKPSID
                     LVPLRGGGPPGTPPLLPIRSDLAVFGPMARSAADLTVALAVLAGPDEMTDGIGYKLTL
                     PPARHTVLKDFRVLLLDTHPLCPTANSVKGALDQLADRLSQTGCAVSRTSRRLPDLAR
                     TTQLYGELLLASYMTDLPATVRQRAEDAAQALSPEDESLAAYRLRGLTISHPDWVRAN
                     RTRAVLRQRWQDLFNDFDVVLCPVMPTPAFPHDHSPKPMRRLDIDGKLVAYDDQIVWA
                     GIATLFGLPATVAPIGHSEVGLPIGVQIIGGFLEDLTTIAFAEQVEREYGGFVPPAMQ
                     "
BASE COUNT          390 a          636 c          632 g          368 t
ORIGIN      
        1 gctaacgcat ctgcgcgagg cgaaagacta cggttctcaa actggacgcg gtttagagcg
       61 gtggcggcta caagatattg tgtttcatcg gagtttttcc gcagcctgtt aaaccccatg
      121 cgggggcgac gagcatcgcg atctcgcgaa agggagcatc aatggggaac gatccgaata
      181 ggcgcttctt cttagaaggc gtcggcgccg tggctatcgc cggctctgtc gccgccccgc
      241 gcaggctgac aagggccgtc gcggctcccg gctctccaat cggatcgccc tatagccatg
      301 cgaccgaact caccacggcg ctcacaaaca agaccgtgtc gtcccgcgaa ctcgttgacg
      361 acgccatcgc tcgcatcgag accctcgaca cgaaagtcaa cgccgttgtc gtgcgggatt
      421 tcgcggccgc ccgcgaggcc gcgaaggcgg ccgatgaggc gctggcgcgt ggcgagcggc
      481 ggccactgct ggggctgcca atgacggtga aggagagttt caacgtcgct ggcttgccga
      541 ccacctgggg caacccaaaa tccaggaatt ggcggccaga cgctgatgcc ctggcggtat
      601 cgcggttaaa ggcggcggga gcggtccttc ttggcaagac taacgttccc atcgatttgg
      661 cggactggca gagctacaac gagatctacg gcacgaccaa taatccctgg gatctcaccc
      721 gcacccccgg cggatcgtcc ggtgggtcgg cggcggcgct cgcggccggg ttcgtcgcac
      781 tcgagctcgg ctccgatatc ggtggttcat tgcgggcgcc ggcgcatttc tgcggtgtgt
      841 tcgcgcacaa accgagcatc gatttggtgc cgttacgcgg tggcggtccg cccggaacac
      901 cgccgttact tccgatacgc agcgatcttg ccgtctttgg gccgatggcg cgtagcgccg
      961 cggatctgac agttgcactg gcggtgctgg cgggacccga cgagatgacg gacggaatcg
     1021 gctacaagct cacgctaccg cccgctcgcc acaccgtgct caaggatttt cgtgtgctgc
     1081 tgcttgatac gcacccactc tgtccaaccg cgaacagcgt gaagggcgcg ctggatcagc
     1141 tggctgatcg cctcagccag accggctgcg cggtttcgag gacgagcagg cgcctgccgg
     1201 atcttgcgcg gaccacgcaa ctttacgggg aactgctcct ggcttcttac atgacggatc
     1261 tgcccgccac ggttcgccaa cgcgccgaag acgccgccca agcattgtcg ccggaagacg
     1321 agagtctcgc ggcataccgt ctgcgcggcc tgacgatctc acatcctgat tgggtccggg
     1381 cgaaccgcac ccgggctgtg ttgcgtcagc ggtggcaaga cctgttcaat gacttcgatg
     1441 tcgtgctgtg cccggtgatg ccgaccccag cgtttccaca tgatcattcg ccgaagccca
     1501 tgcggcggct cgatatcgac ggcaaactgg ttgcctatga tgaccagatc gtgtgggccg
     1561 gcatcgcaac attgtttggt ctgccggcaa cggtggcacc gatcggccat tcggaggtcg
     1621 ggctgccgat cggggtgcaa atcatcggtg gcttccttga agatctcacc acgatcgcat
     1681 tcgccgaaca ggtcgaacgc gaatacggcg gcttcgttcc accagcgatg cagtaaggtc
     1741 cgatgtgaaa cgggacagag ccgagcggcg caaggtcatg gcgctcgccc agccgcagat
     1801 tgatacggtg ctggtggccg aactgtccgc tggggccatc tgatcgagaa ctacctcgcc
     1861 aaactgaagg agttccgctc catcgccacg cgctatgaca agaccgccga aagcttcagc
     1921 gccaatatca agctcgccgc cgccatcatc gcagccagat aatgtcaaca ggccctaaaa
     1981 ggcgcagaaa ctttaagaac gggcgacgga agcaaacact cccgcc
//
LOCUS       DASWQN010001318         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1318251, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001318 DASWQN010000000
VERSION     DASWQN010001318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318251"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VGC82_22370"
     CDS             <1..1115
                     /locus_tag="VGC82_22370"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEY0426078.1"
                     /translation="YSPQDVEARHWLAKRLTEAGLEASIDGIGNVFGRDPTPGRKLLI
                     GSHMETQNHAGWLDGAMGVIYGLEVARALGRGIDVGCWADEEGHFGSFLGSRSFCGLL
                     TEHEIDVCSNRDNGTSLRDALRQAGFSGRPRRQIDTARYRGYLEAHIEQGAELEDHHL
                     RLGIVTAIVGSRRFRIQFKGQQNHAGTTRMAARKDAGVALVKLVAAIEARFPAVAGPR
                     TVWTTGDIKLDPGAASIVPGGAELVFQFRDTNPAILDALDAALTELLDEAAKGPCEVT
                     VAYRGTTAPSVMDAGFQGALEEAAKRHAPGLYQPMPSGAGHDAQILSQLLPSAMLFVP
                     SIGGISHHYAENTRDEDIVLGCQVFSDAAAAILATA"
     gene            1179..1985
                     /locus_tag="VGC82_22375"
     CDS             1179..1985
                     /locus_tag="VGC82_22375"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0426079.1"
                     /translation="MTQPPVLVFIDDCGVATITLNRPEVGNAYNAAMLDALIEGLTNL
                     AADAAVRCLVIRGAGRHFQAGADIGWLNEVAHYPPAENYAASIATTRAMQLLNEFPKP
                     TIALVHGACFGGGVGIVCCADVAFATPDALFGITEVRVGVAPTPISTHMVNAIGLRHT
                     RRYALTGDRFDAKEAERIGLIHEVVAEDAMETKLAGVLDAVFLGSPTAIAMTKSSFLG
                     ANGLLLDERQIATLAHEGWMQRYSPDGHEGTAAFREKRTPLWYQPAHPRV"
     gene            2113..>2215
                     /locus_tag="VGC82_22380"
     CDS             2113..>2215
                     /locus_tag="VGC82_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426080.1"
                     /translation="MVIFGAGGDLTKRLLVPALYNLATTGLLPEQFQL"
BASE COUNT          397 a          691 c          716 g          411 t
ORIGIN      
        1 cgtattcgcc gcaggatgtc gaagcccgcc actggcttgc taaacggctg accgaagctg
       61 gactcgaggc gtctatcgac ggtatcggca atgtttttgg ccgcgacccg acgccggggc
      121 gcaagctgct gatcggcagc catatggaga cccagaatca cgcgggatgg ctggacgggg
      181 caatgggcgt catctacggc ctcgaagtcg cccgcgcgct cgggcgaggc atcgatgtcg
      241 ggtgctgggc cgacgaggag ggccatttcg gctccttcct cggcagccgg tcattctgcg
      301 gcttgctgac cgagcatgag atagacgtct gttcgaaccg ggataacggg acgtcgttgc
      361 gggacgcctt gcggcaggct gggttttccg gccggccgcg acggcagatc gataccgcgc
      421 gctaccgggg ttacctggaa gcgcatatcg agcagggcgc cgaactggag gatcaccatc
      481 tccggcttgg catcgtaacc gcgatcgtcg gtagccggcg tttcaggatc cagttcaagg
      541 gccagcagaa ccacgccggt acgacccgca tggccgctcg caaggacgcc ggcgttgccc
      601 ttgtgaaact ggtcgccgct atcgaggcgc ggtttcccgc cgttgccggg cctcggacgg
      661 tttggaccac gggtgacatc aagctcgacc ctggtgcggc cagcatcgtg ccagggggtg
      721 ccgaactggt gtttcagttc cgcgatacca atccggcgat tctggacgcc ctggacgccg
      781 cgctgaccga attgctggac gaagccgcga aaggtccttg cgaggtgacc gttgcttatc
      841 gcggcaccac cgccccaagc gttatggatg cgggctttca gggcgctctg gaagaagcgg
      901 ccaaacgcca tgctccggga ctatatcagc caatgccatc gggtgccggc cacgatgccc
      961 agatcctgtc gcaactgctg ccttcagcca tgctgttcgt ccccagcatc ggcggcatca
     1021 gccatcatta tgcggaaaac acgcgcgacg aggatatcgt cctcggctgc caggtgttca
     1081 gtgacgcggc ggcggctatt ctcgccacgg cataagggtc ccgtccacgc atgaggttcc
     1141 agctttcgga cggcctgcag caggaataag gagccatcat gacccagcca cctgtccttg
     1201 ttttcatcga cgactgcggc gtcgccacga tcacgctcaa caggccagag gtcggcaatg
     1261 cctacaatgc ggccatgctg gatgccctga tcgaagggct gacgaacctc gccgctgatg
     1321 ccgccgtccg ctgcctcgtg atccgcggtg cgggcaggca cttccaggcc ggcgccgata
     1381 tcgggtggct gaatgaagtc gcccattatc cgccggccga gaactatgcc gcctccattg
     1441 caaccacgcg agcgatgcag ttgttgaacg agtttccgaa accgacgatc gcactggtgc
     1501 atggtgcttg cttcggcggt ggggttggta tcgtgtgctg cgccgatgtt gccttcgcga
     1561 cgccggacgc gttgttcggc attaccgagg tgcgggttgg ggtcgcgccg acgccgattt
     1621 ctacccatat ggtcaacgcg atcggcctgc gccacacccg ccgctatgcc ctgaccggtg
     1681 atcgctttga tgccaaggag gcggagcgga ttggcctgat ccacgaagtc gtcgccgaag
     1741 acgcaatgga gacgaagctg gccggtgttc ttgacgcggt cttcctcggc agcccgaccg
     1801 cgatcgccat gaccaaaagc agcttcctcg gcgccaacgg tctgctgttg gacgaacgtc
     1861 agattgctac gctggcgcac gaagggtgga tgcagcgtta ttcaccagac ggtcacgaag
     1921 gcacggctgc tttccgcgag aagcgcacgc cgttgtggta ccagccggct caccccaggg
     1981 tgtagccaag tcgggaacgg gggacctcat ttgccgggaa tgacagacgc gaactcgacc
     2041 acagtgcatc ctcggggcga ggtgctgaac gctccgccac ggcgagcggt gaaaccacca
     2101 ggaccttgcg ccatggtgat ctttggagcc ggcggcgacc tcaccaagcg cctgttggtg
     2161 ccggcactgt acaaccttgc caccaccggt ttgctgccgg agcagttcca gttga
//
LOCUS       DASWQN010001319         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_394471, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001319 DASWQN010000000
VERSION     DASWQN010001319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394471"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VGC82_22385"
     CDS             <1..263
                     /locus_tag="VGC82_22385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426081.1"
                     /translation="ARSYGGSISVRYVSAILLIRLRYCRRRCLCCQDLRQVRLQAACR
                     PAGEALPRRRRPCRWTDVDDQAAPPDPRLWHRQCRITAFVAP"
     gene            complement(343..696)
                     /locus_tag="VGC82_22390"
     CDS             complement(343..696)
                     /locus_tag="VGC82_22390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426082.1"
                     /translation="MTEQPDQSTRQQEAAIAGPGVIAGQQANPQSGARGIASGALPDV
                     TGPTTHHIGEVAVGTPGMGTDQAGIAPGGTGMSGQPTNDQSPGGGRRDDADQGRTPPD
                     MVTTPGSENRSGQAD"
     gene            complement(758..1096)
                     /locus_tag="VGC82_22395"
     CDS             complement(758..1096)
                     /locus_tag="VGC82_22395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426083.1"
                     /translation="MEPPHDRTAVRKDLHPDVGEFVWTQGNNYFGWRSLLGREPASLE
                     LARQGEVHLAQDGVFTPIHVRGPSADPGADGGEPAASLPLVPSRQVTRRHLVHSAGRT
                     DGTTLTFPMN"
     gene            complement(1331..>1603)
                     /locus_tag="VGC82_22400"
     CDS             complement(1331..>1603)
                     /locus_tag="VGC82_22400"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEY0426084.1"
                     /translation="PHRRSQSELLILAQWPDPAETERNVRWVRAFFEAMQPFAAGGVY
                     VNDLGEEGEARVRAAYGANYDRLVALKNKFDPTNLFRMNHNIAPAA"
BASE COUNT          263 a          521 c          488 g          331 t
ORIGIN      
        1 aagcccggtc ctacggcgga agcatcagcg tccgctacgt ttctgcgatc ctgttgatcc
       61 gcctgcggta ttgccgccgc cggtgcctat gctgccaaga cttgcgccag gtccgcctcc
      121 aggcagcgtg ccgcccggct ggagaggcgt tgccacgtcg ccggcggccg tgccgctgga
      181 ccgatgtgga cgatcaggcg gcaccgcctg atccccggct gtggcaccgc cagtgccgga
      241 taacggcgtt cgttgcccct tgagcttcga acgatcattt ggattgtcat ccactgccat
      301 attctccaat ctcgttgtcg gtcatcagcg tccgggatgc gctcaatcgg cttgtccaga
      361 gcggttctcg gaacctgggg ttgtcaccat gtcaggaggt gtgcgtccct gatcggcatc
      421 gtccctgcgt cccccaccag gtgactgatc gtttgtgggt tgtcccgaca tcccggtgcc
      481 gccaggtgcg ataccagcct ggtcggtgcc catccctggc gtgcccacgg cgacctcgcc
      541 gatgtgatgg gtcgtcggcc cggtaacgtc aggtagtgca ccggatgcaa tgccgcgcgc
      601 gccgctctgc ggattcgcct gttgcccggc aatgacaccc ggcccggcga tcgccgcctc
      661 ttgctgccgc gtggattggt ccggttgctc ggtcatggtg gtctccctct tgcttcgaat
      721 agaacgagag ccggggttca cggttgacca aagattccta attcatcgga aaggtcagcg
      781 ttgttccgtc ggtacgccct gcgctatgaa ccaagtggcg gcgcgtgact tggcgggatg
      841 gcaccaaggg caagcttgcc gccggttcgc cgccatccgc gcccggatca gcggacggcc
      901 ccctcacgtg aatgggcgtg aagacaccat cctgcgccag gtgcacttcg ccttgccggg
      961 ccagttccaa actggctggc tccctgccga gcagcgaacg ccagccgaaa tagttgttcc
     1021 cctgcgtcca cacgaattcg ccgacatccg gatgcaagtc cttgcgcact gccgtgcggt
     1081 cgtgcggcgg ttccacgacg gatcgaacgc cagcgaaccg atcagcgcgt ctttttcggc
     1141 gtggcccagg tggttgaggt cgggaagctc gggcacgccc tcgtttagaa tcgactcgac
     1201 cgtgaccgcg tcaacatctt ttgcagaccg tcagacggca ggtaacctgg ccctcgaaat
     1261 ccgacttaaa ccagggttga gcagatacgt ccttattctg tcttgacgct aacaatccag
     1321 tagaaggttg tcacgccgcc ggcgcaatat tatggttcat tcgaaacaag ttggttggat
     1381 cgaacttatt cttcagagcc accaggcgat cgtagttcgc cccgtaggcg gccctcaccc
     1441 gagcctcccc ttcttcgccc aaatcgttga cgtagacccc gcccgcggcg aagggttgca
     1501 tcgcctcgaa gaaggcgcgg acccagcgga cgttgcgctc cgtctcagcc gggtcaggcc
     1561 actgggccag gatcaggagt tcgctctggg acctgcgatg tgg
//
LOCUS       DASWQN010001320         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_525191, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001320 DASWQN010000000
VERSION     DASWQN010001320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525191"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VGC82_22405"
     CDS             complement(<1..651)
                     /locus_tag="VGC82_22405"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEY0426085.1"
                     /translation="MAPTLLRQVLGLLLLMLLTRCEAVQGGCKLTMITEMPLQVQDRL
                     LVVPAGINGKWTQLVVDSGAERTTISDATATRLSLPHDARFTNRAMGIGGATTTTDVT
                     LDRLVLGGVHFPIDRLAVGTFKLQNDRGLSADGLLGADILLAFDLDIDVPNNRLTFYR
                     TRTCANAQPPWQEPWIEIAGVQARRDRLLLPFELDNATGLAILDTGAQGNVLGVDMA"
     gene            767..>2276
                     /locus_tag="VGC82_22410"
     CDS             767..>2276
                     /locus_tag="VGC82_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY0426086.1"
                     /translation="MTDPITEELFRNAISAIGDEMVLTIYRTAYSGVLKNIMDYSAAI
                     CDAEGRLVAQGLSLPGHLCSIPVALQAVLRHFGDDVHQGDIFINNDPYDGGMHLPDIF
                     IFRPLFAAGRPIAYAATICHHTDIGGRVPGSNASDSTEIYAEGLRIPPLKLYEKGQAN
                     PTLFRMIERNVRLPGRVFGDIRSQLAACEIAARGMTDLVARYGGADGVTTLMVAMMDY
                     SERLTRQCLLELPDGEATFTDWIDDDQIDMGKPIALVCTIRKHGDTMEVDWTGSAPQV
                     KGAINNTLSYTAAMSFTAVKSVLSVNMPNNDGVFRPIRIIAPAGTITHGKLPAACAAR
                     GLTGFRGVDCAFGALAQLYPDKVFAASDGGNTGLTIGGYDKELNPFIYVDFISGAWGG
                     RPWADGLDGNTCMFANMASFSVEVIEAENPLEVLDYEFVPDTGGAGRFRGGMAQRKTW
                     RMLADEGILQVRADRQTHRPYGLQGGGPGAYGRNVLDPGLPTEAVLHAKLTMT"
BASE COUNT          409 a          720 c          757 g          390 t
ORIGIN      
        1 cgccatgtcc acaccaagca cattgccctg cgcgccggta tccaggatcg caagacccgt
       61 cgcgttgtcc agctcgaacg gcaacagcaa ccggtcccgg cgtgcctgga cgccggcgat
      121 ctcgatccac ggctcctgcc agggcggctg ggcgttcgcg caggtccggg tgcggtagaa
      181 cgtcagcctg ttgttcggca catcgatatc aaggtcgaac gccagcagga tgtcagcacc
      241 aagaaggccg tcggcactca gcccgcggtc gttttgcagc ttgaaggtac cgacggcgag
      301 ccggtcgatt gggaagtgca cgccaccaag caccagccgg tccagggtca cgtcggtggt
      361 ggtggtggct ccgccgatgc ccatggcgcg gttggtgaac cgggcgtcgt gcggcaagct
      421 cagccgggtt gccgtcgcat cgctgatcgt ggttcgttcg gcgccgctat cgaccacgag
      481 ctgcgtccat ttcccgttga tgccggccgg caccacgagc aggcgatcct gtacctgcaa
      541 cggcatttcg gtgatcatcg tcagtttgca gccgccttgc accgcctcgc atcgcgtcag
      601 aagcatcagg agcagcaggc caagcacctg acgaagcaaa gttggagcca tcaaatcggg
      661 agcctcgggt gacggatcgt tcctgttgca taacacggcg ggcaggtcgg ttgccacgaa
      721 accgcatcgg tggtcggata atccgccacc cgaggaccac cgccgcatga ccgatccgat
      781 taccgaagaa ctgttccgca atgcgatctc cgccatcggc gacgagatgg tgctgacgat
      841 ctaccggacg gcctattcgg gcgtcttgaa gaacatcatg gactacagcg cggcgatctg
      901 tgatgccgaa gggcggctgg tggctcaggg cctttcgctg ccggggcatt tgtgctcgat
      961 ccccgtggcg ttgcaggctg tgctcaggca cttcggcgac gacgtgcatc agggcgatat
     1021 tttcatcaac aacgacccct atgacggggg catgcacctg ccggatatct ttatcttccg
     1081 gccattgttc gcagccggac ggccgatcgc ctatgccgcg acgatctgcc accacaccga
     1141 tatcggtggg cgggtgccgg ggtcgaacgc ttcggacagc accgagatct atgccgaggg
     1201 actgcggatt ccgccgttga agctgtacga gaagggccag gcgaacccga ccttgttccg
     1261 catgatcgaa cgcaacgtcc ggctgccggg ccgggtgttc ggcgacatcc gctcgcagct
     1321 cgccgcctgc gagatcgcgg ctcgcgggat gaccgacctg gtcgcccgct atggtggggc
     1381 cgacggggtc acgacgctga tggtcgcgat gatggattat tcggagcggc tgacccggca
     1441 atgcctgctg gagctgccgg acggcgaggc gacgttcacg gactggatcg acgacgacca
     1501 gatcgacatg ggcaagccga tcgcgctggt ctgcacgatc cgcaagcacg gcgacacgat
     1561 ggaggtcgac tggaccggca gcgcaccgca ggtcaaaggc gcgatcaaca atacactcag
     1621 ctatacggcg gccatgagct ttaccgcggt gaagtcggtg ctgtcggtca acatgccgaa
     1681 caatgacggg gtgttccgtc ccatcaggat catcgcgccg gcggggacga tcacccatgg
     1741 caagctgccg gcggcgtgtg ctgcgcgagg gctgaccggg ttccgcggcg tggactgcgc
     1801 gttcggcgcg ctggcgcagc tttatccgga caaggtgttt gcggcgtccg acggcggcaa
     1861 caccggcctg accatcggcg gctacgacaa ggagctcaat cccttcatct acgtcgactt
     1921 catctccggc gcctggggcg gtcggccctg ggccgacggg ctggacggca acacctgcat
     1981 gttcgccaac atggccagct tctcggtcga ggtgatcgag gcggagaacc cgctggaggt
     2041 gctggactac gaattcgtgc cggacaccgg cggcgccggc aggttccgcg gcggcatggc
     2101 acagcgaaag acctggcgga tgctggccga cgagggcatc ctgcaggtcc gcgccgatcg
     2161 tcagacgcat cggccgtacg gcttgcaagg cggcggaccc ggcgcatacg gccgtaacgt
     2221 gctggacccc ggattgccga ccgaggccgt gctccatgcc aaactgacca tgaccc
//
LOCUS       DASWQN010001321         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2241764, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001321 DASWQN010000000
VERSION     DASWQN010001321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241764"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..396
                     /locus_tag="VGC82_22415"
     CDS             175..396
                     /locus_tag="VGC82_22415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426087.1"
                     /translation="MADPDVASAAFARAWSAYRLIHPNVHQNDDKRASLERFIRQRCE
                     AGIDDPEMLAVAGLKFLKQLDQSGTPWSD"
     gene            504..731
                     /locus_tag="VGC82_22420"
     CDS             504..731
                     /locus_tag="VGC82_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426088.1"
                     /translation="MKKLMMIGAMAFAATTFANSAMAAEFYVVRDATTKKCTVVDTKP
                     TTTTTTVVDNGIYKTKTEAETGMKTTKVCTE"
     gene            1712..2245
                     /locus_tag="VGC82_22425"
     CDS             1712..2245
                     /locus_tag="VGC82_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0426089.1"
                     /translation="MPALPTWPDEVYRVLKDAGVKQVAMVPDAGHSRLIRSFEADPET
                     RVVTLTTEEEGVAMLAGAWLGGQRGVLLLQSSGVGNCINMLSLPTICHMPLLMIVTMR
                     GDWGEFNPWQIPMGQGTRAALESVGVIVDKVDEPDLVASAVQGAAHLAFNTWKPVALL
                     IGQRVLGAKNFKELARK"
BASE COUNT          508 a          720 c          723 g          399 t
ORIGIN      
        1 aaggggcatt caccaccatg attgttgtct gccgagcccc gaatcgctga ttcaaagcga
       61 gtagccgacc caacgggaaa aatcagcaat tgttccaccc gaactgagct agccaagatc
      121 gggaaaaaat ggcaaccagc cccgctgtcg gtgttgtcat aacgggggaa gtggatggct
      181 gatccagatg tcgcgagcgc cgcctttgcg cgggcctgga gtgcctatcg gctgatccat
      241 ccgaacgtcc accaaaacga cgacaagcgc gccagtctcg agcggttcat tcgacaacgc
      301 tgcgaggcgg ggatcgatga tccggaaatg ctggcggtcg ccggcctcaa gttcctcaag
      361 caactggacc aatccggaac cccatggtcg gactagccca gccgaagcct tccggtcgcg
      421 gcatagacga atcgggaaaa agtggaacat cgggccggga tgcgggttga cggtggcaat
      481 cgtgcaaacc ggaggacgac cccatgaaga aacttatgat gatcggcgca atggctttcg
      541 ctgcgaccac ctttgccaat tcggcgatgg ccgctgaatt ctacgtggtt cgcgacgcca
      601 ccaccaagaa gtgcaccgtg gtcgatacca agccgaccac cacgaccacc accgttgtcg
      661 acaacggcat ctacaagacc aagacggaag ccgaaaccgg catgaagacg accaaggtct
      721 gcaccgagta acgacgggcc ttccaggaga gaaagggccg cggcatcgct gcggcccttt
      781 ttgtttgccg gcgatcacgc ggcaggccgt tgcaagctgc accttcagga ttgcttgcat
      841 cctggttccg tgaagttcag cggacgcgcg ggtccggccg caaggacgaa ttgctgtatc
      901 gccctgcgac aaatcaaccc gacgggcaaa tcacttctga gtttcagaaa tagtgtcaag
      961 gccgggaatc aaaaatattc ctgttgccgc gtcgggcaaa tcagatgtct aacttcgccc
     1021 gtctaccgcg acaagagggg cgatcgcgat cgtcacgacc gtgcggtggg atgcgatgga
     1081 cgcgaggtca cgactgacga gcgtggcttt aagcgtacgg cgaaatcgtg agggtccgac
     1141 gccccggtgc tggcgtcaag ttggcggaag cgaaagccga tgccggcgat ggtggcaaca
     1201 aagccggtca ccaggacgac cacgtataag ccgtaaagcc attgcgcagg gaaggccgga
     1261 tgcctccgcc aaacctgtat gctcatgtgc agcatcttgt tgcaactatc gcacatgaga
     1321 ccgcgggtgc ggcgcgcacc cggtcttccc tgcgccctct ttagtgaaga gggcaaacga
     1381 aatgcaaaac tccgggcaga tcatgccgcg agaacaatct cggctgtcat cgtccgcgaa
     1441 ggcggacgat ccagtattcc agaggccgca gtgaattaac cgataggctg cggtgtactg
     1501 gttcgcccgc cttcgcgggc gatgacagca gaaatactgc ccccatccca gcctttcaat
     1561 tttgcaatag aagccatgct gacattgaaa taccttgccc cggcgcggcg ctctaaggtc
     1621 cgcgccaatc acgccccaaa ccccctttga gggactcgcg gaggaatacg gcccgatgac
     1681 cgatgttgca gcgaaatcga aaggcgcggc cgtgccggcg ctgccgacct ggccggacga
     1741 ggtctatcgc gtcctcaagg acgccggcgt caagcaggtc gcgatggtgc cggatgccgg
     1801 ccatagccgg ctgatccgct cgttcgaggc cgacccggaa acccgcgtcg tcaccctgac
     1861 caccgaggaa gagggcgtgg cgatgctggc cggtgcctgg ctcggcggcc agcgcggcgt
     1921 gctgctgctg caatcgagcg gcgtcggcaa ctgcatcaac atgctgtcgc tgccgacgat
     1981 ctgtcacatg ccgctgctga tgatcgtgac catgcgcggc gactggggcg agttcaaccc
     2041 gtggcagatt ccgatggggc agggcacccg tgcggccctg gaatccgtcg gcgtgatcgt
     2101 cgacaaggtc gatgaaccgg atctggtcgc ctccgcggtg cagggcgcgg cgcacctcgc
     2161 cttcaacacc tggaagccgg tcgcgcttct gatcggacag cgcgtgctcg gcgccaagaa
     2221 cttcaaggag ctcgcccgca aatgacttcg caaaccaatc caaacgcgct gctgcatcgc
     2281 cgcgacgtcg tcaacgaact ggtgcgcgcc cgcggcgaac tgctggtgat cgccggcctc
     2341 ggcgcgccga
//
LOCUS       DASWQN010001322         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_130384, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001322 DASWQN010000000
VERSION     DASWQN010001322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130384"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..761
                     /locus_tag="VGC82_22430"
     CDS             195..761
                     /locus_tag="VGC82_22430"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEY0426090.1"
                     /translation="MDALELMLSRESALKLEAPGPSADELDRIFASAVRAPDHGRLRP
                     WHFVVIDTERRAAFGQLMADSLHRRDPSVPEAGLQRERDKAFRSPIIVAVAAKVQRGH
                     KIPEIEQIASASASAQTIMLAATALGYGAVWKTGEAAYDPIVKTALGLAAEDDIIGFL
                     YIGTRAGGSSPFPRPEARDFVTTWSGHA"
     gene            complement(1327..2436)
                     /gene="tcuB"
                     /locus_tag="VGC82_22435"
     CDS             complement(1327..2436)
                     /gene="tcuB"
                     /locus_tag="VGC82_22435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HEY0426091.1"
                     /translation="MQETEAVIDARREMEICNACRYCEGFCAVFPAMELRREFSAGDL
                     SYLANLCHNCRGCYYACQYAPPHEWGINVPAVLAEVRAQSYAEYAWPKALASAFDRNG
                     TLLSLIAALGIAVVLILSMLLNTQDSFFGAYDTAPRSFYNVIPLWAMQVVSIATFGFS
                     LVALAMGTRNFWRDAGPKGKIRAGDVAIAVWDVLTLKNLGGGGNGCNDNSEAFSMRRR
                     YLHHAMFYGFLLCLASTITAFFYHTFRDWPAPYPFISVPVLFGTVGGILMIVGTVGLL
                     AMKFVDDPMPAVRRLLGADVAMLMLLAMIAVTGLVLLAVRATGAMGLAMAVHLGFILA
                     FFVAIPYSKMVHAIYRSAALVRRAAERNMKPLGGE"
     gene            complement(2423..>2806)
                     /locus_tag="VGC82_22440"
     CDS             complement(2423..>2806)
                     /locus_tag="VGC82_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0426092.1"
                     /translation="PTKVRETIDGFNKAIKPGQVFDNLKLDGNATDGLTPNKTNWARS
                     VDKPPFYGYPLRPGITFTYLGVRVNEKAQVLMDNGQPAGNLFASGEIMSGNILGKGYL
                     AGFGMTIGTVFGRIAGQEAARRARN"
BASE COUNT          546 a          894 c          864 g          502 t
ORIGIN      
        1 gaccggctac acgggcaatg cgaccgtgca tcatggacgt gtcgatcctg gagtcccatt
       61 cttgatggca acggtcgctg aaatgcgcat accgccgatc acgaagggcg ccaatcgcca
      121 gccgccgcga agccgtaggg cactgattgg cgaactgctg acttacgctt aggctgtgga
      181 gaacggagga acaaatggac gcactcgaac tgatgctgag ccgggagtcg gcgctgaagc
      241 tggaagcccc cggcccctcg gccgatgagc tcgacaggat ctttgcaagc gctgtgcggg
      301 caccggatca tggcaggttg cggccgtggc acttcgtcgt catcgacacc gaacgccggg
      361 ccgcgttcgg acagctcatg gcggactcgc tgcaccgacg ggacccatcg gtgcccgagg
      421 ctggtttaca gcgcgagcgc gacaaggcgt tccgctcgcc gatcatcgtc gcggttgccg
      481 cgaaggtgca gcgcgggcac aagattccgg agatcgagca gatcgcctcg gcgtccgcct
      541 cggcgcaaac aatcatgctg gcggcaaccg cgctcgggta tggcgcagtg tggaaaaccg
      601 gcgaagcagc gtacgatccg atcgtgaaga cggctctcgg tttggccgcc gaggacgata
      661 tcatcgggtt tctgtatatc ggcacgcggg caggcggttc ttcaccgttc ccgcgaccag
      721 aggcccgcga tttcgtgacc acctggtcgg ggcacgcctg accaccgcga acgacgcgcg
      781 accggaaccg aggcctaaca gactgcggaa agccctccgg cacttccagc ctgctctcgt
      841 tcgcctcgat gtttggacgg cgctgatact ggaaggctgg acgctgttcg cttcatcgac
      901 tccgtccgct tcatcgacgc cgtccgtgcc tgtccaacgt ttgcttgaaa cgcagagcgc
      961 gccgagatcg ctgggaagtt tctcatgcgc ctgtgaatgc cgcacctgtt gatccgccac
     1021 accccgccgc gaagcggccc aggccaaagg tcccgatcat caacggcgag gcaggattac
     1081 ttctttgtgc cctccatgat ctcggcgccc tccgcgtcaa gcctttgcca gtcgccgcac
     1141 tgccctctaa gcgatgccca cccgatccca cgaatccgca ccatcaagac ccggcgtaaa
     1201 atctgcctcg gcattgaaaa tgcgcttcaa agcgtcaagc cggtacccgg cacaggatcc
     1261 accaagacgc ctccgctctc cgccacgcat gccgggccag tacgtcgcct ctaagccgag
     1321 ctggtattac tccccgccca gcggcttcat attgcgttcg gctgctcgcc gcaccagcgc
     1381 agcggaacgg taaatggcat gcaccatctt cgagtatggg atcgctacaa agaatgccag
     1441 gatgaaccct aggtgcaccg ccatcgccag tcccatggca ccggtcgccc gcaccgccag
     1501 gagaaccaat ccggtcacgg cgatcatggc cagcagcatc aacatcgcga cgtccgctcc
     1561 gagcaggcgc ctcacggcgg gcatcgggtc atcgacgaac ttcatcgcca acaggccgac
     1621 cgttccgacg atcatcagga tgccgccgac cgtgccgaac agcaccggca cgctgatgaa
     1681 cgggtagggc gcgggccagt cgcgaaacgt gtggtagaag aacgccgtga tcgtcgaggc
     1741 caggcacagc aggaaaccgt agaacatggc gtggtgcaaa taacgccggc gcatcgagaa
     1801 cgcctcggaa ttatcgttgc atccgttgcc gccgccgcca agatttttca gtgtcagcac
     1861 gtcccataca gcgatcgcta cgtcgccggc gcgaattttt cccttcggcc cggcgtctcg
     1921 ccagaagttc cgggttccca tcgccagcgc cacaagcgaa aagccgaacg tggcaatgct
     1981 gacgacctgc atcgcccaaa gcgggatgac attgtagaac gatcgcggcg cggtgtcgta
     2041 ggcgccgaag aagctgtcct gggtgttcaa cagcatgctc aggatcagca ccaccgcgat
     2101 gccgagtgcc gcgatcagcg acagcagcgt tccgttgcgg tcgaatgccg aggccagagc
     2161 tttcggccag gcatattcgg cgtagctttg cgcgcgaacc tcggccagta cggcgggcac
     2221 gttgatgccc cattcgtgcg gcggcgcgta ttggcatgcg tagtaacagc cgcggcagtt
     2281 atggcagaga ttggccaggt agctcaggtc gccggcggag aactcccggc gcagttccat
     2341 ggcggggaag acggcgcaaa agccttcgca gtagcggcag gcattgcaga tttccatttc
     2401 gcgccgagca tcgatgacgg cctcagtttc ttgcacgacg agctgcctcc tggcctgcga
     2461 tgcgaccgaa gaccgtcccg atcgtcattc cgaacccggc gaggtagcct ttgccgagga
     2521 tattgcctga catgatttcg ccggatgcga agagattacc ggcgggttgg ccgttgtcca
     2581 tcagcacctg ggccttttcg ttcacgcgga cgcccaggta ggtaaaggtg atgccggggc
     2641 ggagcgggta accatagaac ggcggcttgt ccacggagcg agcccagttg gtcttgttcg
     2701 gggtcaggcc gtcggtggcg ttgccatcca gtttcaggtt gtcaaacacc tgcccgggct
     2761 tgatagcctt gttgaagccg tcgatggttt cgcggacctt tgtcgg
//
LOCUS       DASWQN010001323         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1450464, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001323 DASWQN010000000
VERSION     DASWQN010001323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450464"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VGC82_22445"
     CDS             <1..975
                     /locus_tag="VGC82_22445"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0426093.1"
                     /translation="TAAFVLHSYVAMLFGIGVNRALRVIMSYVMHPYRPALSLKSWRG
                     LAGYSTWTWLLSLAVLVRDRGDSLLLGRLMNIASVGFYSVGAEIAALPTTELIEPLGR
                     AAFSGFAASRQQQTEVGETFLRLIGSAALLTLPAGVGLSLVAAPLVALAFGPGWEPAV
                     PALRILSLSFSVMVFGHLSLHLLSAHALLGRLVGITLTGAAVRIVLLALLIPPFGMVG
                     AATGAAIGVLLEQVLTVLTALRRFRIDPRAIGQLIWRPGLAVSCMATVLFGTGLGWTD
                     DADPMLLAEAVFAGAAIYTAVLLAAWALAGRPAGSETDLLTVLRRAVR"
     gene            complement(1051..1764)
                     /locus_tag="VGC82_22450"
     CDS             complement(1051..1764)
                     /locus_tag="VGC82_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEY0426094.1"
                     /translation="MTWSILARDPLTGTLGAAVATRFFAVGALAIHVEGGVAALATQA
                     LINPMYAVQGMPRLRAGETPDGVIEALLAADAGRAHRQIHILDADGRLACHTGADCVG
                     WCGSIHGIDVSVAGNMLAGAAVVAATLSAFESTKGSMAERLLAALEAGEAAGGDKRGK
                     QSAALKVCSRDPYPDLDIRTDDHPDPLAELRRLFAVSQERFALFRRFLAGEDSPCGVF
                     DRDVIEAAILAHDHAGRNV"
BASE COUNT          299 a          740 c          671 g          399 t
ORIGIN      
        1 acggcggctt ttgtcttgca ttcctacgtc gcgatgctgt tcggcatcgg cgtcaaccga
       61 gcgcttcgcg tgatcatgag ctatgtcatg catccgtacc gccctgcctt gtcgttgaaa
      121 tcctggcgcg gcctggcggg ctattcaacg tggacctggc tgctcagcct ggcggtgctg
      181 gtccgcgacc gcggcgatag cctgcttctg ggccggctca tgaacatcgc ctcggtcggt
      241 ttctattcgg tcggtgccga gatcgcggct ttgccgacaa ccgaactgat cgagccgctg
      301 gggcgggcgg cgttttccgg tttcgcggcg agccggcagc agcaaacgga agtcggcgaa
      361 acctttctcc gcctgatcgg atcggcggcg ctgctgacct tgccggccgg cgtcggccta
      421 tcgctggtgg ccgcgcccct ggtggcattg gcgtttggcc cggggtggga gccggcggtg
      481 ccggccctgc gtatcctgtc cctgagtttc tccgtcatgg tgttcggcca tctgagcctg
      541 cacttgctga gcgcccacgc acttctgggc cgcctcgtcg gcatcacgct gaccggtgcg
      601 gccgttcgga tcgtgctgct ggcgctgctg atcccgccgt tcggcatggt cggcgccgcc
      661 accggtgccg ctatcggcgt cctgctggag caggtgctga cagtcctcac cgcgctgcgc
      721 cggttcagga tagacccgcg agcgatcggc caacttatct ggcgtccggg actggcggtt
      781 tcctgcatgg cgacggtgct gttcggcacg ggattgggct ggacggacga tgccgatccg
      841 atgctgctgg ccgaagccgt gtttgcaggt gcggcaatct acaccgccgt gctgctggcc
      901 gcctgggcgc tggccgggcg gccagcaggg tctgaaaccg accttttgac cgtgctgcgt
      961 cgcgccgtgc gctagaccgt catcttatcg accgccatct tgtcgaccgc catcttgtcg
     1021 accgtcgtct tgtcgaataa gtcgcgtgct tcaaacgttc cgcccagcgt gatcatgcgc
     1081 taggatcgcc gcctcgatca cgtcccggtc gaacaccccg cagggactgt cctccccggc
     1141 caggaagcgg cggaacagcg cgaaccgctc ctggctaacg gcaaacaggc ggcgcagttc
     1201 ggccaacgga tccgggtgat cgtcggttcg gatatcgaga tcgggataag ggtcgcggct
     1261 gcaaaccttc aacgcggcgg actgcttccc gcgtttgtcg cctccggccg cctcccccgc
     1321 ctccagcgct gccagcaacc gttcagccat ggagcccttg gtgctttcga acgccgacaa
     1381 cgtggcggcg acgaccgccg cgccagccag catgttgccg gcgaccgaga catcgatccc
     1441 gtggatcgac ccgcaccagc cgacgcagtc cgccccggta tggcaagcga gccgtccatc
     1501 cgcatcgagg atatggatct gccggtgcgc gcggcccgca tcggccgcca gcagcgcctc
     1561 gatcacgccg tccggcgttt ccccggcgcg cagccgcggc atcccctgca cggcgtacat
     1621 cggattgatc agcgcctgag ttgccaacgc ggccacgcca ccctcgacat ggatcgccag
     1681 cgcgccaacc gcgaagaacc gcgtcgcgac agcggccccc agggttccgg tcagcggatc
     1741 ccgagcgagg atcgaccatg tcattgcgtg cgctccgtgg ttgcggtgca ttttcaacgg
     1801 ccggccagca tcgtcccact ccgaggagat gtcacccgcc ggctttgacg ccgccatatc
     1861 gtccgcccga acgcactaat ccgttacctt gctgccgccc ttgatgaccc ggcgcgacga
     1921 cccgccccgc cggggttatt gtgtcagcat gcaatcgccc cgtccgatcg agaccgacat
     1981 cgtgctgctt ggcgccggcc acgcccatgt cgaggttctt cgccgcttcg ccatgcgccc
     2041 ggaacccggc gttcgcctga ccttgatcgg ccgcgaagcg gaaacgccct ataccggcat
     2101 gctgccagg
//
LOCUS       DASWQN010001324         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_262786, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001324 DASWQN010000000
VERSION     DASWQN010001324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262786"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VGC82_22455"
     CDS             complement(<1..269)
                     /locus_tag="VGC82_22455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0426095.1"
                     /translation="MLFNPYLTFDGRCEEAFRHYKKVFGGDITAMIPHEGTPAAAQVP
                     PEWREKVLHACLSKNGFLLMGSDAPPGHFQQSQGVAVHAATKTVE"
     gene            complement(613..1449)
                     /locus_tag="VGC82_22460"
     CDS             complement(613..1449)
                     /locus_tag="VGC82_22460"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEY0426096.1"
                     /translation="MADAGEASPHLDQKEQRQAAAGAPMGALVIHEIVRAQGEEELER
                     SFGGLAWSGVAAGLSIGFSFLVQATLEAHLPDAPWRPLVAGFGYSIGFLIVILGRQQL
                     FTETTLTAIIPVLTRPNLKSILLALRVWAIVLTANLAATWVFAAISAFPGVFRPETME
                     AMQTLAAHSMAGPFWHTTLTGGSAGWLIGLMVWLLPSAHASRALIIILLTYVIAICQF
                     PHIIAGSVEAAFGVLTGHATVADYTFDFLLPTLIGNTAGGTILAALLNHAPLTGELSE
                     QN"
     gene            complement(1548..1760)
                     /locus_tag="VGC82_22465"
     CDS             complement(1548..1760)
                     /locus_tag="VGC82_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HEY0426097.1"
                     /translation="MTQNLKPGDKVTWRTSRGETKGTVEQKVTSSTKVKGHTARATEA
                     DPEYKVRSDKTGAEAVHKPDSLSKTR"
     gene            complement(1886..>2497)
                     /locus_tag="VGC82_22470"
     CDS             complement(1886..>2497)
                     /locus_tag="VGC82_22470"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0426098.1"
                     /translation="VDNNESRLQAARRIADILGVERAIDWRVGGLPSLPLMDHEADVT
                     FCVEVIEHVGDAPEVVHDLGRASKDILIISSPNRNFPVIRHDTGLPFCHWLPPSSRNR
                     YAAFFNRLHLQDSNIFWSPAMVSAALPDFERSSRFLQFLSYRHFLRSEKEIRSVPGRA
                     PPLDRTIRKFLLGAAAPLGSNAINVLPNLASTFRRRPVRRTHQ"
BASE COUNT          513 a          720 c          772 g          492 t
ORIGIN      
        1 tcttcgactg tctttgttgc cgcatgtacc gcgacgcctt gggattgttg gaagtggccc
       61 gggggagcgt cggagcccat caatagaaat ccgttcttgc taaggcaggc gtgcagaacc
      121 ttctcccgcc actccggggg aacttgggcc gcggcgggtg tgccctcatg cgggatcatc
      181 gccgtaatgt cgccgccaaa aactttctta tagtggcgga acgcttcctc gcaccggccg
      241 tcgaaggtga gatatgggtt gaacagcatt ttacctctcg atacgacttg aagacgtcga
      301 tggcggatta caggaagggc gggctagcgg attaagttga tatcaacgta tctccgcatt
      361 gtcaaattct ggcttcaccg aaaggcctag ttgccactgc ttcaggtgcg gctatcccgg
      421 tcatgggttg gggagccgtt acgaattcat tacatgacag gttgattcgg cccggcgatg
      481 cgcataagcg aaagcatcag gacacacgaa ccgccgcgcg gttaacggag ttcggatcgc
      541 cggaaatgcc acccccggtc gctgaccgac gccaattccc ataacccgcg gatgatgcgg
      601 caaggcaacc ggtcagttct gttcgcttag ttcgcctgtc aacggtgcgt ggttcagcaa
      661 agcagccaga atcgtgccgc cggcggtgtt cccaatgagc gtcggcagca gaaaatcgaa
      721 cgtatagtca gccacggtgg cgtgcccggt cagcacgcca aaggcggcct ccactgaacc
      781 cgcgatgatg tgcggaaatt ggcagatcgc gatgacatag gtcagcagga tgatgatcag
      841 cgcccgactg gcgtgggcgg agggaagcag ccacaccatc aggccgatca accagccggc
      901 cgaaccacca gtcagcgtgg tatgccagaa aggtccggcc atggagtgag ccgccagcgt
      961 ctgcattgcc tccatagttt ccggtcggaa gaccccggga aatgcggaga tggcagcgaa
     1021 cacccaggtc gccgccaggt ttgcggtcaa cacgatcgcc caaacccgca acgccaacag
     1081 gatcgatttc aggttgggtc gtgtcaggac ggggatgatc gccgtcaggg tggtctcggt
     1141 gaacaactgt tgccggccaa ggatgacgat cagaaagcca atggaataac cgaagccggc
     1201 gaccagcgga cgccacggcg cgtccggcag atgggcctcc aaggtcgctt gcaccaggaa
     1261 ggaaaagccg atcgaaaggc ccgcagcgac acccgaccag gcgaggccgc cgaaggagcg
     1321 ttcgagttcc tcctcaccct gggctcggac gatttcgtgg ataaccagcg cgcccatcgg
     1381 cgctccggcg gcggcctggc gctgctcttt ctggtctaga tgaggggacg cctctccggc
     1441 atctgccatg ttcgctcccg ttgcgatgcc ggggcgtaac gcgcaacgca ggcttgagca
     1501 ggattggccg gcttcaattt accgtttgac agccgacggt cggtcgttca gcgggtcttg
     1561 ctgaggctat ccggtttgtg gacagcctcg gcgccggtct tgtcgctgcg gaccttgtac
     1621 tcggggtctg cttcggtggc tctcgccgtg tgacccttca ctttggtaga ggatgtgacc
     1681 ttttgctcca ccgtcccctt ggtttctcct cgcgacgtgc gccaggtcac cttgtctcca
     1741 ggcttcaggt tttgcgtcat cgcggcctcc ggttgatgga agggcaggtc ggcgcagcgc
     1801 ggccgctgac aagcgtcatg ccgcaatcgt gagtaagcag agtcaccgga ccaatgacga
     1861 tagcgcaata atttgcgcaa ctttgttact gatgcgtgcg ccgcacaggc ctccgacgga
     1921 aggtggacgc caggttcggt aacacgttaa tggcgttgct gcccaggggt gcagccgcgc
     1981 ccagcagaaa tttgcgtatc gtccgatcca gcggcggagc gcggcccgga acgcttcgga
     2041 tctccttctc gctccggaga aaatgccggt agctgaggaa ttgcagaaaa cgggatgatc
     2101 gctcgaaatc ggggagcgcc gctgacacca tagctggaga ccagaatatg ttgctgtctt
     2161 gaagatgtaa tcggttgaag aacgccgcgt atcggttccg cgacgatgga ggtagccaat
     2221 ggcagaacgg tagccccgtg tcgtgacgaa tgaccggaaa gttgcggttt ggactactga
     2281 tgatcaaaat gtccttcgat gcacgtccga gatcatgcac gacttcgggg gcgtcgccga
     2341 cgtgctcgat cacttcaaca caaaacgtga catcagcctc gtggtccata agcggaaggc
     2401 tcggcaatcc accaacacgc cagtcaatcg ctcgttcaac gccaagtatg tcggcaatgc
     2461 gacgtgccgc ctgtaaccgc gactcgttgt tgtccac
//
LOCUS       DASWQN010001325         8265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2110383, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001325 DASWQN010000000
VERSION     DASWQN010001325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110383"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3076)
                     /locus_tag="VGC82_22475"
     CDS             complement(<1..3076)
                     /locus_tag="VGC82_22475"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0426099.1"
                     /translation="MPVSFRRLRISGFKSFAEPTSVEILPGLTGIVGPNGCGKSNVVE
                     ALRWAMGESSARSLRGGEMEDVIFAGTAGRSSRNIAEVTVFLDEAQGRAPPPFHEQAE
                     LEIVRRIERGGGSAYRINGREARARDVQTLFADLASGSRASAMVSQGRVSALVMAKPE
                     DRRAVLEEAANITGLHARRHEAELKLRAAEANMARAEDLRAQLESQLGNLKRQVRQAS
                     RYRNISGAIRAAEAELLSLQRGRVEQVRTRAASALHDAQVAVVAATDAATAAGARSAD
                     AAAALPGLREVESNARTVLERRRIAQEQVAGEEQAARTALAEAQRRLGQIRQDLTHAT
                     QLQRDAASADQRLAAEENTLAQAGEGHDERAASAEAAAIACADAVRLAEMEANGATEA
                     AAEANARAQAAAQLLAQAELRSNRLNDQFRTVSQERERLAAQQVDPAAIARAATETSE
                     AEAAAGAARHAVEQAEQVRASATASLASAREFQATADSARARLAAEAQALGEMLAVRD
                     NERWPPMIDSLSVATGLEAALGAVLGEELTSALDPGAARHWRELSAFNPAPALPGATR
                     PLTEFVQGPPALARSLSHIGLVEDDQTGLTHQTALLPGQSLVARSGAIWRWDGYTIRA
                     GTPTQATVRLQQRNRLAGIRIRLAAAEQEAAASRAARTVAEAASQAATSAEQSARTIR
                     RDCEQKLEKCRSGLANLRNQAATATARLAAADDQLTRMTADRDEAVAALAQVRAATGN
                     LPDIAELRATVDRARTALAAARSREAASRSERDALAREHAVRTNRQRVIVAERAGWTE
                     RAKDAADRVTDLAVRLEEAEAALATLDARPAEIAVRRAQALDELSAAEAHHRRAAEAL
                     SAAATLASEADRASRAAESALAARREDTVRAEGGLQQASHAWGTIAERILERLGANPA
                     LPDPPPDLSQEAEDRARKRLERQLREREEMGPVNLRAEQEADDVEKQMGTIETEREEL
                     TTAIAKLRGSIGHLNREGRERLSAVFQEVDRNFQQLFTRMFGGGRAHLALVGS"
     gene            complement(3094..3708)
                     /locus_tag="VGC82_22480"
     CDS             complement(3094..3708)
                     /locus_tag="VGC82_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0426100.1"
                     /translation="MPFSRRSVLIVLPAGFIAGSLALRPGSAFADTDNPRADRSVGKP
                     DAKVVVGEYFSLTCTHCAAFAQTTFPDIRKNLIDAGTVRWVFHDYPLDQIALTASMVA
                     RYLPVDRYEPFINALFASQNRWAFGRGNDPTEELWKIAGLAGMSRTTFDKAVADKELR
                     DWILSEQKADSDKWKIDSTPSFVVNGQKYAGEMNYEAFRKLIPS"
     gene            complement(3724..4230)
                     /locus_tag="VGC82_22485"
     CDS             complement(3724..4230)
                     /locus_tag="VGC82_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEY0426101.1"
                     /translation="MADDDTRDGMTGAARFVYGPRSVGSLVPGVTRPGFRKHNSASAQ
                     ILADWEIIVGPKVASMTIPRRLDRGTLTIACAGPAAMDLHYMGVEVINRINTHLGGQP
                     VHTLKFTQAGMPRKPAQVRPLPPEAIMEAEAAVADLPDGDLKSALTALGRVVIGRAKH
                     STRRLKKL"
     gene            4259..5302
                     /gene="mutY"
                     /locus_tag="VGC82_22490"
     CDS             4259..5302
                     /gene="mutY"
                     /locus_tag="VGC82_22490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY0426102.1"
                     /translation="MICGGDLLRWYDRHRRRLPWRAEPGETPDPYRVWLSEIMLQQTT
                     VTAVIPYYERFVSRFPTVQALAEAPLDAVLSAWAGLGYYARARNLHECARIVASTGGF
                     PADLEQLQALPGIGAYTAAAVGAIAFAIPAVPVDGNVERVVSRLFAIEEPLPAGKTAI
                     RAAAVRLGTDPAARVRPSDFAQALFDLGAGICTPASPGCAVCPLLAGCAARGLGIQTS
                     LPRKAPKKTRPVRYGVHFWLTDDDGRVLLRTRPPSGLLGGMTELPGTDWRALPWTAAE
                     ALSAAPMRLDWRGVGQVRHGFTHFELIIDLLSAHVPRIEGGGFAHPLDALDDAALPSV
                     MRKCVRIAANGKA"
     gene            5372..6481
                     /locus_tag="VGC82_22495"
     CDS             5372..6481
                     /locus_tag="VGC82_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEY0426103.1"
                     /translation="MKTRAAVARKAGDKLSLETIDLTGPRDGEVLVEIKATGICHTDE
                     YTLSGADPEGLFPAILGHEGAGVVVDVGKNVTSLKKGDHVIPLYTPECRQCEYCLSRK
                     TNLCVAIRATQGRGLMPDGSSRFSCEGETLFHYMGTSTFANFTVLPEIALAKIRPDAP
                     FEKVCYIGCGVTTGIGAVINTAKVEVGAKAVVFGLGGIGLNVIQGLRMAGADQIVGVD
                     LNPGRVPMAEKFGMTHFVNPSEVQGDLVPYLVDLTKGGADYTFECIGNTKVMRQALEC
                     AHRGWGKSVIIGVAGAGQEISTRPFQLVTGRVWMGTAFGGARGRTDVPQIVDWYMDGK
                     INIDDLITHVLPFEQINEGFDLMRRGESIRSVVVY"
     gene            6724..7989
                     /locus_tag="VGC82_22500"
     CDS             6724..7989
                     /locus_tag="VGC82_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0426104.1"
                     /translation="MDEPIQLTRLPSGLTVVTERMDRVETVSIGAYVATGSRNESEAE
                     NGVSHFLEHMAFKGTASRSAAAIAEEVEAVGGHINAYTAREQTAYYVKFLKEDTALGV
                     DIIGDILTHSVFEPEELERERSVILQEIGQANDTPDDIIFDHFQSIAYPHQSLGRAVL
                     GSADAIRNMPRSALTGYMTRNYTNSNVVVAATGKLRHDEILDLVQRHFADLPSFSPPR
                     FDDATYMGGEFREERDLDQVHVVLGFPSAGYSDPDYYATILLSTLLGGGMSSRLFQEI
                     REKRGLVYSIYSFASPFKDGGLFGIYAGTGESEAAELMPVMLEELGKVQAHVTDQELS
                     RARAQVKASLLMSQESTGSRCEQLARQIQTFGRVISTEEVLGKINAVTEADIRRAAIR
                     HFRAAPTLATVGPSAQVPTLANIVETLGA"
BASE COUNT         1328 a         2630 c         2784 g         1523 t
ORIGIN      
        1 ccgacccgac cagcgccagg tgtgcccggc cgccgccgaa catgcgggtg aaaagctgct
       61 ggaagttgcg atcgacctcc tggaataccg cgctcagccg ttcgcggcct tctcggttca
      121 gatgtccaat cgatccgcgc agcttggcga tcgccgtcgt caactcctcc cgctcggttt
      181 cgatcgtgcc catctgcttc tccacatcgt cggcttcctg ttcggcgcgc aggttgaccg
      241 gccccatctc ctcgcgttcc ctaagctgcc gctccaggcg tttgcgggcg cggtcttcgg
      301 cttcctgcga caggtccggc ggtggatcgg gcagcgcggg gttcgccccg agccgttcca
      361 ggatgcgttc ggcgatggtg ccccaggcgt gactggcctg ctgcaagccg ccctcggcgc
      421 gcaccgtatc ctcccgccgc gcggcaagtg cggattcggc cgcccgagaa gcccgatcgg
      481 cctcagaggc cagtgtcgcg gcggcagaca aggcctctgc cgcccgacga tggtgcgctt
      541 ccgcggccga caattcgtcc aaagcctgcg cgcgtcgaac cgcgatctcg gcggggcgcg
      601 cgtccagtgt tgcaagcgcg gcttcggctt cttccagccg aacagcaagg tcggtcacgc
      661 gatcggccgc gtccttcgcc cgttcggtcc atcccgcgcg ttccgcgacg ataacccgtt
      721 ggcggttggt ccgcacggcg tgttcgcgcg ccagtgcgtc acgttcgctc cgcgaagctg
      781 cctcccgcga gcgagccgcc gcgagcgcgg ttctggcccg atccaccgtg gcacgcagtt
      841 cggcgatatc gggcaggttg ccggtggccg cgcgcacctg ggccaaggcc gccaccgctt
      901 catcccgatc ggccgtcatg cgggtcagtt ggtcgtccgc ggcggccagg cgtgcggtcg
      961 cggtcgccgc ttgattgcgc aggttcgcga ggcccgaccg gcacttttcc agtttttgct
     1021 cgcagtcgcg gcggatggtg cgggcgctct gctccgcaga tgtcgcggcc tgcgatgcgg
     1081 cctcggcaac ggttcgcgcc gcgcgggaag ccgcggcctc ctgttcggcc gcggcgaggc
     1141 ggatcctgat gccggccaag cggttgcgtt gctgcagccg cacggtcgcc tgcgtcgggg
     1201 tgccggcccg aatggtatag ccgtcccacc gccagatcgc tccggaccgc gcgaccaatg
     1261 actggccggg aagcaaagca gtctggtgag ttaagccggt ttgatcatct tcgaccagtc
     1321 caatatggga tagcgaccgc gccagcgccg gcggaccttg cacgaactcg gtcaagggtc
     1381 gcgtggcgcc gggcagcgct ggggctggat tgaacgccga cagctcccgc cagtgccgcg
     1441 ccgcaccggg atcgagcgcc gatgtcagtt cctcgcccag taccgcacca agcgccgcct
     1501 ccagcccagt ggcgacgcta aggctgtcga tcattggcgg ccagcgctcg ttgtccctta
     1561 ccgccagcat ttcgccgagc gcctgtgcct cggccgccag cctggcgcgt gccgaatcgg
     1621 ccgttgcctg gaattcccga gcggaggcca atgaagcggt tgccgaggcg cgcacctgtt
     1681 cggcctgttc gaccgcatgt cgcgcggcgc ccgcggcggc ttcggcttcg gacgtttccg
     1741 tggctgcccg ggcgatcgcg gcggggtcca cctgctgcgc cgcgaggcgt tcacgctcct
     1801 ggctgacggt ccggaactgg tcgttcagcc ggttggagcg aagttccgcc tgggccaaga
     1861 gctgcgccgc cgcctgggcc cgtgcattcg cttcggcggc cgcttcggtg gcgccgttgg
     1921 cctccatttc cgcaaggcga accgcgtcgg cacacgcgat ggcggctgct tcggcggatg
     1981 cggcgcgttc gtcatgtccc tcgccggctt gggccaaagt gttttcttcc gcggcgagac
     2041 gttgatccgc cgaagcggcg tcgcgttgta gctgtgtggc gtgcgtcaga tcctgcctga
     2101 tctgccccag ccgccgttgc gcttccgcca acgccgtccg cgcagcctgt tcctcgcccg
     2161 cgacctgttc ctgagcgatg cgccggcgct ccagcaccgt gcgggcgttc gactcgacct
     2221 cgcgcagtcc aggaagcgcg gccgcggcgt ccgccgaccg ggcgccggcg gcggtcgcgg
     2281 catcggtcgc cgccaccacg gcgacctgcg catcgtgcaa agccgaagct gccctggtcc
     2341 gcacttgttc gacccggccg cgttgcagcg acagcaattc ggcttccgcc gctcggatcg
     2401 cacccgagat attgcgatac cgggaagcct gccggacctg gcgcttgagg ttgccaagtt
     2461 ggctctccaa ctgcgcccgc agatcctcgg cgcgtgccat gttggcctcg gccgcgcgga
     2521 gtttcagttc ggcttcgtgg cggcgtgcgt gcaggccggt gatgttggcg gcttcttcca
     2581 ggacggcgcg ccggtcctcc ggctttgcca tcaccagggc gctgacgcgt ccctggctga
     2641 ccatcgcgga ggcgcgcgat ccggacgcca ggtcggcgaa caacgtttgc acgtcgcgcg
     2701 ctcgggcttc gcggccgttg atgcgatacg ccgaaccgcc gccgcgctcg atccggcgaa
     2761 cgatctccag ttccgcttgc tcatggaacg gtggcggcgc gcgtccctgc gcttcgtcga
     2821 ggaacaccgt gacctcggcg atgttgcggg acgaccggcc ggccgtaccg gcgaagatga
     2881 cgtcctccat ctcgccgcca cgcaggctgc gggcgctgct ttcgcccatc gcccagcgta
     2941 gcgcctccac cacattggac ttgccgcagc cgttgggtcc gacgattccg gtcagtccgg
     3001 gcaggatctc cacgcttgtg ggttcggcga agcttttgaa cccggagatg cgaagacggc
     3061 ggaaactgac cggcaatgtt tactccttgg gcgtcatgac ggaatcagtt tccggaacgc
     3121 ctcgtaattc atttccccag cgtatttctg accgttcacc acaaaactcg gagtcgagtc
     3181 gatcttccat ttgtccgagt cggctttttg ctcgctcagg atccagtcgc gcagctcctt
     3241 gtcggcgacg gctttgtcga aggtggtgcg gctcatgccc gcgaggccgg cgatcttcca
     3301 cagttcctcg gtcggatcgt tgccgcggcc aaaggcccaa cggttctggc tggcgaacag
     3361 cgcgttgatg aacggctcgt agcgatcgac cggcaggtaa cgggcgacca tcgaggcggt
     3421 cagggcgatc tggtccagcg ggtagtcatg gaacacccag cgcacggtgc cggcgtcgat
     3481 caggttcttc cggatgtcgg ggaaggtggt ctgggcgaag gccgcgcaat gcgtgcaggt
     3541 gagcgagaaa tactccccta cgacgacttt ggcgtctggt ttgccgaccg aacgatcggc
     3601 gcggggattg tcggtgtcgg cgaaagccga accgggacgc agcgcgaggc tgcccgcgat
     3661 gaaaccggct ggcagcacga tcagaacgga acgacgagaa aatggcatag gaacctcttg
     3721 gttttacagc ttctttaggc gccgcgtcga atgtttagcg cggccgatca caacccggcc
     3781 gagcgcggtc aaggctgatt tcaggtcgcc atccggcaag tcggcgaccg cggcctcggc
     3841 ttccatgatg gcttccggcg gcaagggccg cacctgggcc ggtttccgcg gcatcccggc
     3901 ctgggtgaac ttcaaggtat ggaccggctg accgcccaga tgcgtgttga tccggttgat
     3961 cacctcgacc cccatgtagt gcaggtccat tgccgcgggg ccggcgcagg cgatggtcag
     4021 tgtcccgcgg tcgagccggc ggggtattgt catcgacgcg accttggggc cgacgatgat
     4081 ctcccagtcg gcgaggatct gtgccgaagc ggagttgtgc ttgcgaaagc ccggacgcgt
     4141 gacgcccggc accaagctgc ctaccgagcg cggcccgtag acgaatcggg ctgctccagt
     4201 cataccgtcg cgcgtgtcgt cgtctgccat caatcccgct ccggttaccc cttatccaat
     4261 gatttgcggc ggcgacctgc tgcgttggta cgaccgccac cggcgacgcc tgccgtggcg
     4321 tgccgaaccc ggcgagacgc ctgatccgta ccgcgtctgg ctgagcgaga tcatgctaca
     4381 gcagaccacc gtgaccgcgg taatacccta ctatgagcgc ttcgtaagcc gcttcccgac
     4441 ggtacaggcg ctggcggagg ctccactgga cgcggtgctg tcggcttggg cggggcttgg
     4501 ttactatgcg cgcgcgcgga acttgcacga gtgtgcccga attgtcgcca gcacgggtgg
     4561 ctttcctgcc gatcttgaac agctccaggc gttgccgggc atcggcgcct atactgccgc
     4621 ggcggttggg gcgatcgcgt tcgccatccc ggcggttccg gtggacggca atgtggaacg
     4681 ggtcgtttcc cgcctgtttg ccatcgagga acccttgccg gcgggcaaga cggcgatacg
     4741 ggcggccgcg gttcggctgg gcacggatcc ggcagcccgg gtcaggccca gcgacttcgc
     4801 acaggcgctg ttcgatcttg gggctggcat ttgcacgccg gcttcccctg gctgcgcggt
     4861 ctgcccgctg ctggccggct gcgccgctcg ggggctgggt atccagacat cactgccgcg
     4921 caaggcgcca aaaaagaccc ggcccgtgcg ctatggcgtg cacttctggc tgaccgacga
     4981 cgacggtagg gtgctgctgc gcacgcgtcc acccagtgga ctgctcggcg ggatgacgga
     5041 actgcctggc accgactggc gcgccttacc ctggacggcg gccgaagcgc tgagcgcggc
     5101 acccatgcga ctggattggc ggggtgtggg ccaggtacgg catgggttta cccacttcga
     5161 gttgatcatc gacctgctgt cggcgcatgt gccacggatc gaaggcggcg ggttcgcgca
     5221 tccgctggat gccctggacg acgccgcgct gccgtcggtg atgcggaaat gcgtccggat
     5281 tgccgccaac ggcaaggctt gatagttttc ggacgtatcg catggtcgct ccgggtttgg
     5341 atggaaatcc gggtttgaag ggaaagcgga tatgaaaacg cgtgccgcgg tggctcgtaa
     5401 agccggtgac aagctcagcc tggaaacgat cgacctgacc gggccacgcg acggcgaggt
     5461 ccttgtggag atcaaggcga ccggcatctg ccacaccgat gaatacacct tgtcgggcgc
     5521 cgacccggaa gggttgtttc cagcgatcct ggggcacgag ggtgctggcg tggtggtcga
     5581 tgtggggaag aatgtcacca gcctgaaaaa gggcgaccac gtgatcccgc tgtacacgcc
     5641 ggaatgccgg cagtgcgaat actgcctgtc gcgcaagacc aacctttgcg tggcgatccg
     5701 agcgactcag ggcagggggt tgatgccgga tgggagcagc cgtttctcgt gcgaggggga
     5761 gacgctgttc cactacatgg gcacctccac cttcgcaaat ttcaccgtgc tgccggagat
     5821 cgcgctggcc aagatccggc ccgacgcgcc gttcgagaag gtttgctaca tcggctgcgg
     5881 cgtcaccacg gggatcggcg cggtgatcaa cactgcgaag gtcgaagttg gggcgaaagc
     5941 ggtggtgttc ggactggggg ggatcgggct gaacgtgatc cagggcctgc ggatggccgg
     6001 agccgaccag atcgtcggcg tggaccttaa tcccgggcgg gtgccgatgg cggaaaaatt
     6061 cggcatgacg catttcgtta acccgtccga ggtgcagggc gacctggtgc cgtacctggt
     6121 cgatctgacc aagggtggtg ccgactacac gttcgagtgc atcggcaaca ccaaggtgat
     6181 gcgtcaggcg ctggaatgcg cacatcgtgg ctggggcaaa tcggtcatca tcggcgttgc
     6241 cggagccggg caagagatct cgacgcgccc gttccagttg gtgaccggcc gcgtctggat
     6301 gggcaccgcg ttcggcggcg cccggggacg caccgatgtg ccgcagatcg ttgactggta
     6361 tatggacggc aagatcaaca tcgacgacct gatcacccat gtcctgccgt tcgagcagat
     6421 caacgaaggc ttcgatctga tgcggcgcgg cgagtcgatc cggtcggtgg tggtttactg
     6481 agatcggggt ctctttcaac gtaaccgcct cagagcggtc tccggtcgtg gtgggcaaga
     6541 tgcaccagtt aaaggcgttt gtttgtcccg aacctgtaca gccactggga gacgtcggcg
     6601 tcctgtattg ataaggtatt catagcagac gcgccacgaa gtgctatgtt gtgacggttc
     6661 cggttgcgcc gtccgccgcg accccatatg catggactga catttgaaag agaacgccta
     6721 gccatggacg aaccgatcca actgacccgc ctgccgtctg gcctgacggt cgtcaccgaa
     6781 cgaatggatc gcgtcgagac tgtctcgatc ggcgcctatg tcgccaccgg ctcgcgcaat
     6841 gaatccgaag cggaaaacgg cgtctcccac tttcttgagc acatggcctt caaaggaacg
     6901 gcgtctcgca gcgccgcggc catcgccgag gaagtcgagg ctgtcggcgg tcacatcaac
     6961 gcctacactg cccgtgagca gaccgcgtat tacgtcaaat tcctgaagga agacaccgcg
     7021 ctgggcgtgg atatcatcgg cgacatcctg acccactcgg tcttcgaacc cgaggaattg
     7081 gagcgcgaac gcagcgtcat cctgcaggaa atcggccagg ccaacgacac gcccgacgac
     7141 atcatcttcg accacttcca gtccatcgcc tatccgcacc agtccttggg acgagctgtg
     7201 ctggggtcgg cggatgcgat ccggaacatg ccccggtcgg cgctgaccgg atacatgacg
     7261 cggaactaca ccaacagcaa cgtcgtggtc gccgcgaccg gaaagctgcg gcacgatgaa
     7321 atcctggatc tggtgcaacg gcatttcgcc gatctgccca gcttctcgcc gccgcgcttc
     7381 gacgacgcga cttacatggg cggggagttc cgcgaggagc gcgacctcga tcaggtgcac
     7441 gtcgttcttg ggtttccgtc ggctggttat agcgacccgg attactacgc gacgattttg
     7501 ctctcgacgc ttcttggcgg cggcatgtcg tcgcggctgt tccaggaaat ccgggagaag
     7561 cgcggtctgg tgtactcgat ctattccttc gcttcgccgt tcaaggatgg tggcctgttc
     7621 ggcatctatg ccggcaccgg tgaaagcgag gcggcggagt tgatgccggt gatgctcgag
     7681 gagctcggca aggtccaggc tcatgtcacg gaccaggagc tcagccgagc ccgggcgcag
     7741 gtgaaggcga gcctgctgat gtcgcaggaa agcaccggca gccggtgcga gcagttggcg
     7801 cggcagatcc agacgtttgg acgggtgatc tccaccgagg aagtgttggg gaagatcaac
     7861 gccgtgacgg aagccgatat tcgccgggcg gcgatacggc actttcgtgc agcgccgacg
     7921 ctggcgacgg tcggaccatc cgcgcaggtg ccgacactgg cgaatatcgt ggagacgctg
     7981 ggggcgtagg ttgttgcggg tcgtggcgga cttgatttgt ttagacagga aatccgtgac
     8041 gtaggttcgg ggaacttagt gacagctgag gttcgcggcc gtaatggaac gcgccacagc
     8101 gagagcagct tcacggggat tggaaacagt gtttccttgt ggtcgcaaag aaagtcatgg
     8161 gtggccgtgc caagcacggc catgacccct atggacgtca ggcggcaaaa ctggctcgca
     8221 cctttccagc gagtcgaaag cactccaagc acgatcaagc cggac
//
LOCUS       DASWQN010001326         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1318922, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001326 DASWQN010000000
VERSION     DASWQN010001326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318922"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            427..>1586
                     /locus_tag="VGC82_22505"
     CDS             427..>1586
                     /locus_tag="VGC82_22505"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEY0426105.1"
                     /translation="MTRDTLVLPSLSPIGDKPISAVIDDRCTSANGGLLLFQDVERRL
                     NLATRLATCLRDPRQPGKITHRLDEILRFRMLAIAAGYPDGNDCDVLRDDPIFKMALD
                     RPPVSGPALCSQPTVSRLENMPRRPELYRMAKAMLDLYCDSFPVVPSHLTLDLDESFD
                     RVHGEQELRKFNAYYDDWGFLPIHIFDTSGRLVVSVLREAATPSGREILTLVKRVVGH
                     LRERFPRVRIRLRGDSHYACPEVMSWCEAEAIEYIFGLAGTASLAAQVQTLEGRTAAR
                     YAARRTAEPPKFKLRRYMDFRGGAKSWKCQRRIVARVEAGPDGVDTRSIVTNLTDRRA
                     QRLYERKYCQRGRMENLLKAHKLHLASDRTSCTRACANQVRLFLHGAAYWLL"
BASE COUNT          288 a          515 c          485 g          298 t
ORIGIN      
        1 gcctcttgcc caagcttcaa cggtccgtgg tgcaaccccc agaagcaggg atacccgatt
       61 tgcccaacca ttgtgttgcc cgccgttgaa caggtaccca agcttgcgga cgagtgtggg
      121 aggtaggata aagttagttg gcatagtgct tgaaaaacct tttgcttcac ggatgcgtct
      181 gccagtttaa tgcgtaccga ggggaccgat caggacttcc taaaccaagg ccagttagaa
      241 ccccctcggc tccagctttc caaagccgcc ccgagggcga aaatctggct cgaaatctat
      301 ggttcccgaa ttgaacacgg tttagcccgg attattcacg aaggcggtgg gtgtggcgcg
      361 agcccgtgag cgggcgtagg atttggttgt caacgccaat ccgagccctg ggagcgccca
      421 cccaccttga cgcgagatac gcttgtcctg cccagcctgt caccaatcgg tgacaagccg
      481 atttccgccg tcatcgacga ccggtgcacg tccgccaacg gcggcctcct gctgttccag
      541 gatgtcgaac gacgcctgaa ccttgcgacc cggttggcaa cttgcctgcg tgatccccgg
      601 cagccgggga agatcaccca tcggctcgat gagatcctgc ggtttcgcat gctggcgatt
      661 gcggccggct atcccgacgg caacgactgc gacgtgctcc gggacgatcc catcttcaag
      721 atggcgctgg accgaccgcc ggtctcgggg ccggcgctct gctcgcagcc gaccgtctcc
      781 cgcctggaga acatgccgcg gcgcccggaa ctctatcgca tggccaaggc gatgctcgac
      841 ttgtattgcg acagctttcc ggtcgtcccc agccatctga cccttgatct tgatgaaagc
      901 tttgatcggg tccatggcga gcaggaactg cgcaagttca atgcctatta cgatgactgg
      961 ggttttctgc cgattcatat cttcgacacc agcggccggc tggtcgtgtc cgtgctgcgg
     1021 gaggcggcga cgccgagcgg ccgcgagatc ctgaccctgg tcaagcgggt ggtcggccat
     1081 ctgcgcgagc ggttcccccg ggtccgcatc cgtctgcgcg gcgacagcca ctatgcctgt
     1141 ccggaagtga tgagctggtg tgaggccgag gccatcgagt acatcttcgg cctggccgga
     1201 acggcttccc tggcggcgca ggtccagacg ctcgaaggcc gcacggcggc gcgatatgcc
     1261 gcgcgccgca cggccgagcc acccaagttc aagctccgcc gctacatgga cttccggggc
     1321 ggggccaagt cctggaaatg ccagcgccgg atcgtcgccc gggtcgaagc cggacccgat
     1381 ggggtcgaca cccgctccat cgtcaccaac ctgaccgacc gccgggcgca gcggctctac
     1441 gagcgaaaat actgccagag aggccggatg gagaacctgc tgaaggccca caagcttcac
     1501 ctggcctccg atcggacttc ctgcacgaga gcctgcgcca atcaggtccg gctctttctg
     1561 catggcgccg cctattggct gctctg
//
LOCUS       DASWQN010001327         3790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_395740, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001327 DASWQN010000000
VERSION     DASWQN010001327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395740"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VGC82_22510"
     CDS             <1..1135
                     /locus_tag="VGC82_22510"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY0426106.1"
                     /translation="SVGEVLGWDIGEMAESVTIHAFVRSPFDSYVRDQTRFWNASGVA
                     VKLGGAGVEMQVESLRALLLGGVAFETPATGPSSEVSLANHGFPLFPNQDAAKAASYS
                     RKIPLLGYFNSSVRGLAPGSDVVMHGLKVGKVTDVRLSFDLANDTVLTPVHFELEPER
                     VLGIGQQVFKDAADAVQTMVSKGLRATLQSGNLLTGEMLISLEIIPDAPDVKVTMQDG
                     SFVIPITQSAGLSGLQSSAGDLLRNVNAIPFGSIGQSLDAMVKNMSALTNGPQLQQSV
                     TALAATMSDAQLVMKSLSTDMGPALKQLPQIASSLQSTLKQSDQLLRSVSAGYGDNTQ
                     FHRDLDRLMAQLNDAVRSFQALADLLVRHPEALVRGRTDSGVQ"
     gene            1132..1722
                     /locus_tag="VGC82_22515"
     CDS             1132..1722
                     /locus_tag="VGC82_22515"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HEY0426107.1"
                     /translation="MTYYRRFQSAVALCALLMAASCTSSDPKLYTIAPLPGSPLSDGP
                     KVVALHGVGIARYLQRSQIVQSSEEYRIALRPGDWWGEPLDAMLARVLAEDLTLRLPQ
                     STIYTSSGVVTGSPEATVEIEVQRLDLDRDGNLLLIAQGSVSFKSRSSPDTRSFRISE
                     PPRSPGAAGQVAATSIALGELADRIAGMLVVRSGRK"
     gene            1719..3020
                     /locus_tag="VGC82_22520"
     CDS             1719..3020
                     /locus_tag="VGC82_22520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEY0426108.1"
                     /translation="MSSPSTAEIAADRVEAALRECPGCGFFQIEPVLAPGTTAQCERC
                     GTMLRKARQHPLEHSMALAVAALILLTVMCLTTLMTVRTSGIVHQAGIFSGPVELVQR
                     GMSGLAIVVIFVTVAAPLAKLLGTIYVLLRVRETDPPRHLRRIFTLVERLSTWSMTEV
                     FVLGVFVAYVKLGDLVKIELDAGVYALLALTFVLVWADGSLDREAIWDALDGGRARDV
                     ARRALVTGRVPPGAIGCETCTLVVVPDGPEGCPRCGSAVHARKPDSIAWTWALLIGAM
                     VFYLPANLYPVLTVMQLGAGQPSTILGGVRELIETGMYPLAALVFFASIAVPMLKMVG
                     LSVMLISVHTGHMHWLRDRTRLYYLIHWIGRWSMIDIFMEALLGALVQFGGVVTIEPG
                     IGAVAFCAVVILTIFAAETFDPRIMWDAAERRTNISKQAQA"
     gene            complement(3011..>3790)
                     /locus_tag="VGC82_22525"
     CDS             complement(3011..>3790)
                     /locus_tag="VGC82_22525"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0426109.1"
                     /translation="ILFLTTPGPPIFCNGFEIGYGDVLLCGPNVTFLSRLSGHTNWAS
                     MTLSADDMEAISTSYFGGSQTHIDGLRVITPPAAALAHLRSMHECIESSIEAAGELLD
                     CGAAQGRLEQILITAMLNIINEAGVRSDTMARQHHHIVINRFFEILDANLGKSLNMQV
                     VSKEIGISSRTFRMACQEQLGICPTQYLLLRRMGLARRALQQNDPTLTTVTDIATELG
                     FWELGRFAVKYHEIFGETPSTTLRDCNSKGTRLRIGNHVHA"
BASE COUNT          736 a         1071 c         1119 g          864 t
ORIGIN      
        1 atccgttggc gaggtgttgg gttgggatat cggcgaaatg gcagaaagcg tcaccatcca
       61 cgcattcgtg cgctcaccct ttgattcgta cgtccgtgac cagacccgtt tctggaatgc
      121 gtctggtgtg gcagtgaaac tggggggcgc tggcgtcgaa atgcaggtgg aatcgctccg
      181 cgcactgctt ttaggcggcg ttgcctttga aacgccagcg actggaccat ccagcgaagt
      241 cagtttggcg aaccatggtt ttccactatt cccgaaccag gatgccgcca aggctgcttc
      301 ctacagccgg aaaatcccgt tgcttggtta cttcaattcc tccgtgcgcg gtcttgcccc
      361 aggatcggat gtcgtcatgc acggcctgaa ggtcggtaag gtcaccgatg tcagactaag
      421 cttcgatctc gcaaacgaca ccgtgctgac gccggtacat ttcgaacttg agcccgagcg
      481 tgttctgggt atcggacaac aagtattcaa agatgccgcc gacgcggttc agacgatggt
      541 gtcgaaaggg cttcgtgcaa cgctgcaaag tgggaacttg ttgaccggtg aaatgttgat
      601 ctcactcgaa atcatcccgg acgcgcctga tgtcaaagtg acgatgcagg atgggtcgtt
      661 cgtgatccca attacgcagt ccgccggatt gtcgggtctc cagtcgtccg ccggtgatct
      721 attgcgcaac gtaaacgcca ttccctttgg cagcatcggc caaagcctgg atgccatggt
      781 caagaacatg agtgcgctca ccaacggtcc gcaactccag cagtccgtga cagcactggc
      841 cgccactatg agcgatgcgc agttggtgat gaagagcctg agcaccgaca tgggtccggc
      901 attgaagcaa ctcccgcaga tagccagctc gctgcaatcc acgctgaagc aatcagacca
      961 gctgctgcga tcggttagtg cgggttatgg cgataacaca cagtttcatc gtgatctgga
     1021 ccggctgatg gcgcaactaa acgatgcggt gcgttcgttc caggcgctgg ctgatctgct
     1081 ggtaaggcat ccggaagcgc tggtgcgtgg ccggacggat tcaggtgtgc aatgacgtat
     1141 tatcgccggt tccagtctgc ggtggctctg tgcgccttgc tgatggccgc ttcgtgcacg
     1201 tcatccgatc cgaagcttta taccatcgct ccgttgcctg gatcgccctt gtccgacggc
     1261 cccaaagttg ttgcgctgca cggggtgggt atcgcgcgct acctgcagcg tagccagatc
     1321 gtgcagtctt cggaagaata ccggatcgcc ctgaggccag gcgactggtg gggcgagccg
     1381 ctcgacgcga tgttggctcg cgtgctggcg gaggatttga ctctgcggct gccccaaagc
     1441 acgatctaca cgtcctctgg cgttgtcacc gggtctcctg aagcaacagt cgagattgag
     1501 gttcagcggc tcgatttgga tcgggatggc aacctgctcc tcatcgccca gggctcggtg
     1561 tcattcaaga gccggtcatc gcccgatacg cgtagcttcc gcatatcgga gccaccgcgg
     1621 tcgccagggg cggcaggcca ggtcgcggca acgagtatag cgcttggcga actggcagac
     1681 cgcatcgccg gcatgcttgt cgtcaggtcg ggtcgaaaat gagttcgccg tccaccgcgg
     1741 aaatagcggc ggaccgagtg gaggctgcgc tccgcgaatg tcccggctgc gggtttttcc
     1801 agattgagcc tgtgcttgcg ccaggtacga cagcccaatg cgaacgttgc ggaaccatgc
     1861 tgcgcaaagc ccgccagcac ccgctggagc atagcatggc gctggccgtt gccgccctga
     1921 tcctgctgac ggtcatgtgc cttaccacgc tcatgaccgt gcgtacatca ggcatcgtgc
     1981 atcaggcggg aatcttctcc ggcccggtgg aactggtgca gcgcggcatg tcgggcctcg
     2041 ccatcgtggt catattcgtc actgtcgcgg caccgttggc caagctgttg ggcaccatct
     2101 acgtgctgct gcgcgttcgc gaaacggatc cgcctcggca tcttcgccgc atattcacgc
     2161 tggtcgagcg gctctccacg tggtcgatga ccgaagtctt tgtgctcggc gtgtttgttg
     2221 cctatgtaaa gctgggtgat ctcgttaaga ttgagctgga cgccggcgtt tatgccctgc
     2281 ttgcgctgac gttcgttctg gtctgggccg acggatcgct ggatcgagaa gcgatctggg
     2341 acgcgcttga cggcggccgc gcccgggatg tggcccgcag agccctcgtc acgggacgtg
     2401 tgccacctgg ggcaatcgga tgcgaaacct gcaccctggt tgtcgtgcct gatgggccgg
     2461 aaggttgccc tcgctgcggt tccgctgtgc atgcccgcaa acccgacagc attgcctgga
     2521 cctgggcgct gctgatcgga gccatggtat tttatcttcc cgcgaacttg tatcctgtcc
     2581 ttaccgtaat gcaacttggt gccggacaac ccagcacgat tctgggcggc gtacgcgagc
     2641 taattgaaac ggggatgtat ccgttggctg cgttggtgtt ttttgccagc atagccgtac
     2701 caatgctgaa aatggtcggt ctcagcgtga tgttgatatc ggtgcacacg ggccacatgc
     2761 attggctccg agaccggacg cgattgtatt acctcattca ctggattggc cgttggtcga
     2821 tgatcgatat attcatggaa gcgcttcttg gcgctctcgt tcaatttggc ggagtcgtta
     2881 ccatcgaacc cggtatcggc gcagtggcgt tttgcgcggt cgtcatcctc acgatatttg
     2941 ctgccgaaac tttcgatcct cgaatcatgt gggatgctgc tgaaagacgg acgaatatca
     3001 gcaagcaggc tcaggcatga acgtgatttc caatcctgag ccgtgtgcct tttgaattac
     3061 aatccctcaa agttgtggac ggcgtctccc caaatatctc atggtatttc acggcaaacc
     3121 gcccaagctc ccaaaagcct aattccgtcg caatatcggt caccgttgtg agcgtgggat
     3181 cgttttgctg caacgcgcgc cgcgccaggc ccatgcgacg aagtagcagg tattgtgtgg
     3241 ggcatatccc cagttgttct tggcacgcca ttcggaacgt ccggctagag ataccgattt
     3301 ccttgctgac cacctgcatg ttcaacgact tgccgaggtt cgcgtccaat atctcgaaga
     3361 accggttgat gacgatgtga tgatgttgcc tggccatcgt gtccgaacgc acacctgcct
     3421 cattgatgat gttcaacatg gcagtgatca atatctgctc gagcctgcct tgggctgcac
     3481 cgcaatccag taattctcca gcggcttcta tcgaactttc gatgcactcg tgcatcgagc
     3541 gaaggtgggc caatgccgcc gccggtggtg tgatgaccct taaaccatcg atatgcgtct
     3601 gactgccgcc aaaatatgaa gtggagatag cctccatatc atcggcagaa agtgtcatgg
     3661 aagcccaatt tgtatgcccc gacaatcgtg ataggaatgt gacattaggt ccgcacagca
     3721 ggacgtcacc atatccgatt tcgaaaccgt tgcagaaaat cggtggcccc ggcgttgtca
     3781 gaaacaatat
//
LOCUS       DASWQN010001328         3487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1978374, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001328 DASWQN010000000
VERSION     DASWQN010001328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978374"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..846)
                     /locus_tag="VGC82_22530"
     CDS             complement(229..846)
                     /locus_tag="VGC82_22530"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEY0426110.1"
                     /translation="MIIEMRTYTLQPGTLAEAEKRFGEALPAREKHSKLAAFWHSEIG
                     PLNQIIHVWAYDSFQHRADVRAAALKEPGWPPAIREFVTSQQSEVFLPAPFSPPLEPR
                     QVGPLFEIRQYTLIPGAIPGLIERWSEKIEGRQKFSPLVGGWYSEFGALNKWVHIWAY
                     KDANERFSVRAAASAGGQWPARNPPGVVVKQENALVMPAAFSPIR"
     gene            complement(915..1457)
                     /locus_tag="VGC82_22535"
     CDS             complement(915..1457)
                     /locus_tag="VGC82_22535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426111.1"
                     /translation="MGSAYGTGWHLPLAWMAWLATTDLRLWVYEQRALYGAMRFLHLA
                     GMASFVGGVAVNEFRRMNVSASTVVLPVRGSLIALMHGSFGIVLLSGVWLFLRDPLGM
                     GLHTMFLPKLALICAGAVYAHVARHPGLRLAGNPGKHMVALTSALLWMTVVGLSTWNH
                     VERPVNVNAALRATNLGRNQ"
     gene            1568..2341
                     /locus_tag="VGC82_22540"
     CDS             1568..2341
                     /locus_tag="VGC82_22540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426112.1"
                     /translation="MRPIRPASVMFAALLFGTAASAQALEAASPDALRAGQAQGAAAS
                     DRAADMDFLFRLAMVEGHLIVGHDLLAAREGDLALPHFGHPVRELYDDLSEYLEARKF
                     PAFDTQLLKLEAAAASAPYAAATEAQYTAVIETVHKAREIAPADLRASVPAMIKVCAE
                     TLDAASGEYNGSLEGGQVKLLVEYHDSRGYVSWVSQYVDSLIAGHKDAASQDLLGRMK
                     TIAGKAQWIVEALLPAPAPRASVDQYRAVAVEAAALSKQ"
     gene            2426..3145
                     /locus_tag="VGC82_22545"
     CDS             2426..3145
                     /locus_tag="VGC82_22545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEY0426113.1"
                     /translation="MTWSIVAHDPNTGAFAVAVATKAFAVGASCPFVRAGVGAVSTQS
                     MTNRYLGPAVLDAMQRGLPPEAAIEGALAGDNGSGLRQVHAVDSQGRTAAWTGQNCVE
                     WCGGVSAGGISVAGNMLAGEPTVAATLAAWKANHQLPMPDRLMLSMEAGEAAGGDRRG
                     KQSAAMVMVTTEDFPDLNLRVDDHPEPLRELRRLLAIWKVEGVPRLGIAPSKANPSGL
                     TDLDAIEAVWIAKGMDLRFRR"
BASE COUNT          652 a         1087 c         1096 g          652 t
ORIGIN      
        1 gtgagcctcg gtatcggcca tgacacggcg gagttcgtcg tgaatgcggt tcgccgctgg
       61 catcaagcga ttggttctgc ccgctgtccg aaggtggatc gacggctgat caccgccaat
      121 ggcggtggct caaacggatc gcggagaccc gtaattcggc gtgcgccggc gaaccggcga
      181 gcgtggcaac caagccggag ctgcaacgtt cgccggtatg ttccggttct accggattgg
      241 cgagaacgcc gccggcatca ccaacgcatt ctcctgcttc accacgacgc ccggcggatt
      301 ccgagccggc cattggccgc cggccgaggc ggctgccctt acgctgaaac gctcgttggc
      361 gtccttgtag gcccagatgt ggacccattt gttgagcgca ccgaactccg agtaccagcc
      421 gccgaccagc ggcgagaatt tctgccggcc ttcgattttt tccgaccagc gctcgatcag
      481 tccggggatc gcacccggga tcagcgtgta ctgccggatc tcgaacaatg gcccgacctg
      541 gcgtggttcc agcggcggcg agaacggagc cggcaggaag acctcgctct gctgcgacgt
      601 cacgaactcg cggattgccg gcggccagcc gggttctttc agtgcagcgg cgcgtacatc
      661 ggcacgatgt tgaaaactgt cgtacgccca cacatggatg atctggttca gcggcccgat
      721 ctcgctgtgc cagaacgcgg caagcttgga gtgcttttcg cgcgccggca gggcttcgcc
      781 gaaacgtttc tcagcctcgg ccagtgtgcc gggctgcagc gtgtaggtcc gcatttcgat
      841 aatcatgttt cgtctccccg tgatagccac gccaagctac ggcaagtgcc gcgttgcggg
      901 aagggcgggc gaggtcactg attccggccg agatttgtag cccgcagcgc ggcattcacg
      961 ttcaccggcc gctccacatg gttccaggtg gagaggccga ccaccgtcat ccacaacagt
     1021 gcggaagtaa gcgccaccat gtgctttccc gggttcccgg ccagccttag cccaggatgg
     1081 cgcgcaacat gggcgtaaac cgcgccagcg cagatcagcg ccagtttcgg caggaacatc
     1141 gtgtgcaaac ccatccccag agggtcgcgc aaaaacagcc ataccccgct tagcagcaca
     1201 atcccgaagg agccgtgcat cagggcaata agtgaaccgc gcaccggcag cacaaccgtg
     1261 gacgcggaca cattcatccg acgaaactcg ttgacggcga cgccgccgac gaagctcgcc
     1321 attccggcca aatgcaggaa ccgcatcgcg ccatacagcg cgcgctgttc ataaacccaa
     1381 agccgcaaat ccgtcgttgc cagccacgcc atccatgcga gcggtagatg ccacccggta
     1441 ccataagcac ttcccatcgc gatcctgtca gtcctgcttg cggtgtgtat tgaccctgct
     1501 tgcggtgcgt attgacacgg cgggccgcta gacgcaaatg agagttattc gcagacagga
     1561 aatcagaatg agaccgattc ggcccgcctc cgtgatgttc gcggcactgt tgttcggcac
     1621 cgcggcatcg gcgcaagcgc tagaggcggc ttcgccagac gctttgcgcg ctggccaggc
     1681 ccaaggtgcc gccgcgtccg atcgggccgc cgacatggat tttctgtttc ggcttgcgat
     1741 ggtggagggt cacctgatcg tcgggcacga cctgctcgcc gctcgcgagg gtgatctggc
     1801 actcccgcat tttgggcacc cggtgcggga actatatgac gatctgtcgg aatatctgga
     1861 ggcaaggaag tttccggcgt tcgacactca gctattgaag ctggaagccg ctgctgcctc
     1921 cgcgccctat gccgccgcga ccgaagcgca atataccgcg gtgatcgaga ccgtccacaa
     1981 agcgcgcgaa attgcccccg ccgacctgcg agcctcggtt cctgccatga tcaaggtttg
     2041 tgccgaaacg ttggacgcgg catcgggaga atacaacgga tcgctggaag gcgggcaagt
     2101 caaacttctt gtcgagtatc atgacagtcg tggctacgtt tcctgggtct cccagtacgt
     2161 ggacagcctg atcgcgggcc acaaggacgc cgcttcccaa gatctgcttg gtcgaatgaa
     2221 gacaattgcc ggaaaagccc agtggatcgt cgaagcgttg cttccggctc cggcacctcg
     2281 tgcttcggtt gaccaatacc gagccgtcgc ggtcgaagcg gctgctttgt cgaagcagtg
     2341 aagggattgc cgggttggtg cggccgctgg acggtggcgc atgcctgacc tacgaactgg
     2401 ctcctgacat cgcgggagct cggttatgac ctggtccatc gtcgcccatg atccgaacac
     2461 gggagccttt gccgtcgctg tcgcaaccaa ggcgtttgcc gtgggtgctt cctgtccctt
     2521 cgtgcgggcc ggtgtcgggg cggtgtccac ccaatcgatg accaaccgct accttggccc
     2581 cgcggttctg gacgccatgc agcggggttt gccgcctgaa gcggcgatcg aaggggcttt
     2641 ggcgggcgac aacggaagcg gcctgcgtca ggtccatgcg gtggacagcc agggccgcac
     2701 cgccgcgtgg accggacaga actgcgtgga gtggtgcggc ggtgtgtcgg ccggcggtat
     2761 cagtgtcgct ggcaacatgc tggcggggga accgacagtc gccgccaccc tggcggcatg
     2821 gaaagccaac catcagcttc cgatgccgga ccggctgatg ctgtcgatgg aagccgggga
     2881 ggccgccggc ggcgaccggc gcggcaagca atccgccgcg atggtcatgg tcacgaccga
     2941 ggatttcccc gacctcaatc tgcgcgttga tgaccatccg gaaccgctgc gggagctccg
     3001 ccgcttgctg gcgatctgga aggtggaagg cgttccccgc ctcggtatcg cgccatccaa
     3061 agccaatcca tccgggctga ccgacctcga tgcgatcgag gccgtttgga ttgccaaggg
     3121 catggatctg cgcttccggc ggtaggacgc gtagcctcga tcattttcag cctgactgtg
     3181 caggccgcaa ccggtagatg gagggccttc gccaccaaga caggatgatc cgatcatgca
     3241 gcaatcgaag caactggacc atgatcgttt gatgttgaac cgtccccgat gttcgaccaa
     3301 ggcctaaccc tatttggttg cgtaatttac gctttctgac tgggtcaacc tcaggcgatt
     3361 acgctaatca tgaccagacc cggagtgtgt ctcaggtccc ggcgaccacg tccggtgacg
     3421 gaacgcccgc gttacggcgc tattcgataa cgggaatgct gtcggaatca ccagtcggtt
     3481 ggcgtca
//
LOCUS       DASWQN010001329         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1319544, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001329 DASWQN010000000
VERSION     DASWQN010001329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319544"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VGC82_22550"
     CDS             complement(<1..1207)
                     /locus_tag="VGC82_22550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019092009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEY0426114.1"
                     /translation="MLTASAATRREHDLLGEADVPVSAYWGIHSQRAVENFPITGVPV
                     GHFPNLVRALALVKLAAARANKRLGHLDAGKAAAIEKACLLIAEEGRFHDQFVVDAVQ
                     GGAGTSTNMNANEVIANIGLEFMGQERGHYAALHPNDDVNMAQSTNDTYPTALRLSVI
                     FAAMPLAQALDDLAFAFKTKSVEFADVLKMGRTQLQDAVPMTVGQEFDAFHATLKEDV
                     ARLGEIGGLFREISLGATAIGTGINADPRYASLAVEELARAARQPLVPATNLIEATSD
                     MGAFVLFSGLLKRIAVKLSKICNDLRLLSSGPRTGIGELRLPAVQAGSSIMPGKVNPV
                     IPEVVNQVAFLVIGHDLTVTMCAEGGQLQLNPFEPTIGYCVLNSLRMLTAAVETLRTR
                     CVEGIEVDRE"
     gene            complement(1405..>1912)
                     /locus_tag="VGC82_22555"
     CDS             complement(1405..>1912)
                     /locus_tag="VGC82_22555"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0426115.1"
                     /translation="AIQVAKAFGAKVIATAGSEAKRQACLEEGADHAIDYRSGFGDRV
                     KELTGGRGVDIVYDPIGDKVADESLRCLAWCGRLLILGFLGGGPTNIRSNYLLIKGID
                     AIGVRIGGLNEANPDLAVANMKALIVLAEQGKLRPRISHTFRLDQAAEALQVVIDRAV
                     IGKAVLLS"
BASE COUNT          333 a          646 c          577 g          356 t
ORIGIN      
        1 gttcccggtc cacctcgatg ccctcgacgc atcgtgtgcg tagcgtttcc accgccgcag
       61 tcagcatccg cagtgaattc agcacgcagt agccgatcgt gggttcgaac gggttcaact
      121 gaagctggcc gccttcggcg cacatcgtca ccgtcaggtc gtgaccgatg accaggaacg
      181 ctacctggtt gaccacctcg gggatcaccg gattgacctt gcccggcatg atcgaggacc
      241 ccgcctgcac cgccggcaac cgcaactcgc caattccggt ccgcggcccg ctcgatagca
      301 ggcgcaaatc attgcagatc tttgacagct tcaccgcgat gcgtttcagc aatccggaga
      361 acagcacgaa cgcccccatg tccgaggtcg cctcgatcag gttggtggcc ggcaccagcg
      421 gctggcgggc ggcccgagcg agctcctcga ccgcaagact ggcgtagcgg ggatcggcgt
      481 tgataccggt cccgatcgcg gtcgcgccca ggctgatttc gcgaaacaat cccccgatct
      541 cgcccaggcg ggccacgtct tctttcagcg tggcatgaaa agcatcgaac tcctgtccga
      601 ccgtcatcgg caccgcgtcc tgaagctgcg tgcggcccat tttcaacaca tcggcgaact
      661 cgacggactt ggtcttgaac gcgaaggcca ggtcgtccag cgcctgggcc agcggcatcg
      721 cggcgaaaat caccgacagg cgcagcgcgg tggggtacgt gtcgttggtc gattgcgcca
      781 tgttcacgtc gtcattggga tgcagcgcgg cgtaatgtcc ccgctcctgt cccatgaatt
      841 ccaggccgat gttggcgatg acctcgttgg cattcatgtt ggtcgaggtg ccggcgccgc
      901 cctggaccgc atccacaacg aactgatcgt ggaaacgtcc ctcctcggcg atcagcagac
      961 aggccttctc gatggcggcg gccttgccgg catccagatg ccccagccgc ttgttagctc
     1021 gcgcggcggc cagtttcacc agcgccagcg cccgcacgag gttggggaaa tgcccgaccg
     1081 gaacgccggt tatgggaaaa ttctcgacgg cgcgttggct gtgaatcccc caataggcgg
     1141 agaccggcac gtcggcctcc cccaacaaat catgttcgcg ccgcgttgcc gccgaggctg
     1201 tcagcaacgg gttcggcggc tcctgcccgg tacgctcccc agcaacaaga ggtgcgggca
     1261 ccggatcggc ggcttcgttc acgacgcggt ctcctgttcg ggatcagctt gaaccatttt
     1321 cagcctgatc atgcgtgcgg aaagtggtct gtctcgttgg ttgttcgcat gattcacacg
     1381 ccttggacgt tccgccctgc gtgatcagct cagcagcaca gcctttccta taacggcacg
     1441 atcgatgacg acctgcaacg cctcagcggc ctgatccagc cggaacgtat gcgagatccg
     1501 gggccgcagc ttgccctgtt cggccagcac aatgagcgcc ttcatgttgg cgaccgcgag
     1561 gtcggggttc gcctcgttca ggccgccgat gcgcacgccg atcgcgtcga tccccttgat
     1621 caacagatag ttgcttcgga tattggtggg cccgccgccg agaaagccca ggatcagcag
     1681 gcggccgcac catgccaggc agcgcagcga ttcgtcggcg accttgtcgc cgatcgggtc
     1741 gtaaacaatg tccacgcccc gtccgccggt cagctccttc acccgatcgc cgaacccgga
     1801 gcggtagtcg atcgcatgat ccgcaccttc ctcaagacag gcctggcgct tcgcctcgct
     1861 gccggcggtg gcgatgacct tggcgccgaa cgccttcgcc acctggatcg cg
//
LOCUS       DASWQN010001330         5063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_395753, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001330 DASWQN010000000
VERSION     DASWQN010001330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395753"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VGC82_22560"
     CDS             complement(<1..1037)
                     /locus_tag="VGC82_22560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0426116.1"
                     /translation="MSVKADFAMRSIGCTLCAMSLVVGWSLDRAAAQSAPAVPVTTTT
                     AVRRDVPVWLRGLGTVQAYYAVQLRPRVDGTLTQVPVKEGQDVKKGDLLAVIDPRPYQ
                     AALDGALAKKQQDQAQLANAQADLARYSSLVRQDFASRQQLETQQATVKQVSAAIQGD
                     EAQVEMAQLNLSFSYITAPFDGRIGLRNIDPGNIVHSSEAAPILSMTQVQPISVTFTL
                     PQDNFPSIMRAMEQHPLDVVVYGSDNKTELDRGVLLTPDNAIDTTTGTIKLKATFPNA
                     RRTLWPGQFVNVRLLLGIQPNVIAVAATAVQHGPSSLFVYQVGPNDTVAVQPIQVASQ
                     EDDIYVVSSGLT"
     gene            complement(1433..4771)
                     /locus_tag="VGC82_22565"
     CDS             complement(1433..4771)
                     /locus_tag="VGC82_22565"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HEY0426117.1"
                     /translation="MAWHQEGGWFHRSKSHAAVRHTRWAIHHTSRAVHRLLIVGTGLL
                     VVASCLLAGAAWRLAQGPIDLGWLADRVKAELVDDTAPIRVSFDRLALAWEGFHKGVD
                     YPLDLQISNISITDSAGRRLIAAPEAHLTLSFAGILLGRIVPRTIEVDHGQVSVTRDD
                     TGAIDLGWDLTPDDAHGVGPDRAAPGSAGPGSRGPGSRGPVDLRQLREQLAGPANSDR
                     GRSRGLLDQLQRAHFRDTEVSLHDRRSGLTARTSGMDVNLVRTTNGHIRGALEAPLLF
                     GNRAGGDQAGGEHAGLSIAVDFVPGSDGSLDFGLTPVRPAGVDALPPALAVLSCLDVP
                     ISFKGTVAFDAGFTLRHMQLAARAGTGQVLTSQGAIPLRGGLIDLSGTAELVSISKFH
                     LDLSQLTDPLRQAADLSGTIALVSDRLTASLTVGLEQIDIASLPRLWPVGVGGGARSW
                     VTEHVTGGLVTRATASFVIEADEALHDVILTKGTGELDGSNAAFSWLDNVPPIEQAEV
                     HLRLVDPDTLDIRVSAGRQRIANRGADLLVKDGQMRITGLSLSDQNTTIHTRIDGSVG
                     SALALLSEPRLHLLSEHPINLKSSGGEVSATLDLQFPLKTDLPVDDIQIHADAHLRRV
                     KLLDIAGGHVLEDGAFDLGITKEGLSVKGRGLLAAMPVTLDGTMDFSPGPADQVVQRL
                     VVSGRPDASQLDAAGLPLKHVVGGPIPMTAVVIERRNGDGSVQVDGDLTEAALEVGPL
                     AWSKPSGVNASATAALITSHGRLTKIDRIAVRGVGLLLSGSANLADGRLRSVRVDGFQ
                     LGRSQGHGLINIAANDDINIVVQGDQIDLSPKLKQKSSAANVSDGPSATRPVWALNAR
                     FDRAILANEEIASGLLANATGAGDAIRLLDAVGATQTGAGFAIKIEPSAGGRHLLVEA
                     KDAGRFLRGLDAVRVMQGGHLTVDAMFDSPSGFQPLAGKALLNDFIVKDSPLFGKLLQ
                     AITLYGLVDVLRGPGMTFSTAVVPFRYDGTNLNLNEVRAENPSLGLTAQGRIGLSSGQ
                     AAIAGTIVPAYFFNSLPGQLPLVGKLFSPEKGGGVFAARFGIDGLIDDPSITINAASA
                     LTPGFLREIFGVFDRPASAAPPSR"
     gene            4760..>5063
                     /locus_tag="VGC82_22570"
     CDS             4760..>5063
                     /locus_tag="VGC82_22570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426118.1"
                     /translation="MPGQWPTPYDEAAADRLVERFRTLGETESRLLEAAGVLPMLRCL
                     GGNSPYLAALALREPESIHDLATHGPSAVFAHGLSRLSNLSPAAKRPAVAAGLRQAK"
BASE COUNT          989 a         1632 c         1524 g          918 t
ORIGIN      
        1 gtcaggccgc tggaaacgac gtagatgtca tcctcctggc tcgcaacctg aatgggctgg
       61 acggcaacag tatcgttcgg tccaacctga taaacaaaca gactgctggg tccgtgttgt
      121 accgcggttg ccgcaacggc aatgacgtta ggctgaatcc ccaatagcag ccggacattg
      181 acgaactgac ccggccagag cgtacggcgg gcattgggaa aggttgcttt cagcttgatc
      241 gtgccggtgg tcgtgtcgat ggcattgtcc ggcgtcaaca ggacgccccg gtcgagttcg
      301 gtcttgttgt cgctgccata aacgacgaca tccagcggat gctgctccat cgcccgcatg
      361 attgacggaa agttgtcctg cggaagggta aaggtgacgg aaatcggctg gacttgtgtc
      421 atcgacaaga tgggcgccgc ctcagaggaa tggacgatgt tgccggggtc gatgttgcgc
      481 aagccgatgc ggccgtcgaa cggcgcggtg atgtagctga aactgaggtt gagctgcgcc
      541 atttccacct gcgcttcgtc cccctgaatc gcagcggaaa cctgcttcac cgttgcctgc
      601 tgggtttcaa gctgctgacg cgacgcgaaa tcctgccgca cgagagaact atagcgcgcc
      661 agatcggcct gggcgttggc caattgcgcc tggtcttgct gctttttagc caaggcccca
      721 tccagagccg cttgatacgg gcgggggtcg atcaccgcca gcaggtcgcc cttcttgacg
      781 tcctgaccct ctttgaccgg aacctgcgtc agggttccat cgacgcgcgg tcgcaactga
      841 acggcataat acgcttgcac ggtacccaga ccacgcaacc aaaccggcac gtcccgccgc
      901 acagccgtcg ttgtcgtaac aggcaccgcc ggagccgact gggcagcagc ccgatcaaga
      961 ctccagccaa cgacgagcga catagcacac agcgtgcacc cgatcgaccg catcgcgaag
     1021 tcagccttca cagacattcc atttcccatc gcagaaggca tagtttgccc gaacatgata
     1081 cggtggccaa cccggaatcc agatcccgcc aacacccatc acgcggtgat actcgggcat
     1141 cgattagaac aaaaatagcc accaaaggta acaaaatcga tcggtctgtt atcgaagcac
     1201 atcacgatcg cgaactcggt ccgtcacaaa ggcaggtcgc tgactgaagc aagcgtcgct
     1261 gaccgaagca agcaatctgc cgaagccagc tctgctgcct ccgtggtcta gccagccggc
     1321 tccgtggtgc agtcagcccg agtatccaaa atatacttct gaaggctcgc tcggacaatc
     1381 tagcctgacc gttcactctg gcaatgcttc aacgccttgt ttcgtcgcta tttcagcgtg
     1441 aaggcggtgc cgcactcgcg ggccggtcga aaacaccgaa gatttcccgc aggaagcctg
     1501 gcgtcaacgc gctggccgcg ttgatcgtaa tactggggtc gtcgatcaag ccgtcgatac
     1561 cgaagcgcgc cgcgaagacg ccgccaccct tttcgggact gaacaacttt ccgacgagcg
     1621 gcagctggcc aggcagtgag ttgaagaaat aggccggtac gatcgtcccg gctatcgccg
     1681 cctgcccgga cgacaaaccg atccggccct gcgcggtcag gccaagagaa gggttctccg
     1741 cccgtacctc attcaggttc aggttggtgc cgtcgtagcg gaacggcacg acagcggtgg
     1801 agaacgtcat gccaggccca cgaaggacat cgaccaagcc atacagggtg atggcctgca
     1861 acaacttacc gaacaacggg gagtctttga cgatgaaatc gttcagcagt gccttcccgg
     1921 ccagtggctg aaatcctgat gggctgtcga acatcgcatc gaccgtcaga tggccgcctt
     1981 gcatgacccg aaccgcgtcc aggccccgca gaaagcggcc ggcatctttt gcttcgacca
     2041 gtagatgccg tccgccggcg ctgggttcga tcttgatagc gaatccggca ccagtttgcg
     2101 tcgcaccgac agcatccagc aggcggatcg cgtctcctgc ccccgttgcg tttgccagca
     2161 gaccgctcgc gatttcctcg ttggccagga tcgcacggtc gaatcgggca ttcagcgccc
     2221 agacgggccg cgttgccgac gggccgtcgg aaacatttgc ggctgacgat ttctgcttca
     2281 atttgggcga caaatcgatt tgatcgccct gcacaacgat gttgatgtcg tcgttcgcgg
     2341 cgatattgat caatccatgc ccctggcttc ggccgagttg gaaaccatcg actcgcacgg
     2401 accgaaggcg gccgtcggct aagtttgccg acccgctcag aagcagccca acaccccgca
     2461 ccgcgatacg atcgatcttg gtcaaccggc cgtgcgaggt tatcaacgcg gccgtggcgc
     2521 tcgcgtttac ccccgaaggt ttactccagg ccagcggacc gacttcaagt gcggcctccg
     2581 tcaggtcgcc atccacctga accgatccgt cgccattgcg ccgttcgatc acgaccgcgg
     2641 tcatcgggat gggcccgccg acaacatgct tcaggggcaa gccggcggcg tcgagttgcg
     2701 atgcatccgg ccggccggac acaaccagcc tttgaacaac ctggtccgcg gggcccggac
     2761 taaaatccat cgtgccgtcg agcgtgacgg gcatggcggc caacaatccg cgtcccttta
     2821 cactcaggcc ctccttggtg atgccgagat cgaaggcgcc atcctcaagc acatgaccac
     2881 cggctatatc cagcagcttg acgcgcctga gatgtgcgtc cgcatgaatt tggatgtcgt
     2941 cgaccggcag atcggtcttg agcggaaact gcaagtccag cgtcgccgac acctcgccac
     3001 cgctggattt caggttgatc gggtgctccg agagcaagtg cagccgcggt tcactcaaaa
     3061 gtgccagggc gctgccgacg ctgccgtcga tgcgggtatg gatcgttgtg ttctggtccg
     3121 acaacgacaa gccggtgatg cgcatctgac cgtccttgac gagcaggtcg gcaccgcgat
     3181 tggcgattcg ctggcggccg gcagacacgc ggatgtccag cgtatccgga tcgaccagcc
     3241 gcaggtgaac ctccgcttgc tcgatcggcg ggacgttatc gagccagctg aatgccgcgt
     3301 tcgacccgtc gagttcacct gtccccttgg tcaggatgac gtcgtgcagc gcttcgtccg
     3361 cttcaatgac gaacgacgcg gtcgcacgcg tgaccaatcc gccggttaca tgctcggtta
     3421 cccatgagcg ggcaccgccg ccgacgccaa cgggccacaa tcgcggaaga ctggcgatat
     3481 cgatctgctc gaggccgacc gtcagcgacg ccgtcagccg atccgacaca agcgcgatgg
     3541 tgccgctgag atcggccgcc tgacgcagcg ggtcggtcaa ctggcttaga tccaggtgaa
     3601 atttgctgat cgagaccagt tctgccgttc cggacaggtc gatgaggccg ccccgtagcg
     3661 ggatggcacc ctggctggtc agaacctggc cggtacccgc ccgagccgca agctgcatat
     3721 gccgaagcgt gaagccggcg tcgaaggcga cggtgccctt gaacgagatt ggcacgtcca
     3781 gacaggacag gacggcaagc gcaggcggca acgcgtcgac cccagcgggc cggaccgggg
     3841 ttaggccaaa atcgaggctg ccatccgatc ccgggacgaa atccacggca atactcaggc
     3901 ccgcgtgctc gcctcccgcc tgatcgcctc ccgctcgatt gccgaaaagt agcggtgcct
     3961 caagagcgcc acggatgtgc ccatttgtcg tccgcaccag gttgacgtcc atgccggatg
     4021 ttcgtgcggt cagcccggat cgccgatcgt gtagcgacac ctcggtatcg cgaaaatgcg
     4081 cgcgctggag ttggtccaga agtccccggc tccggccgcg gtcactgttg gcagggccgg
     4141 cgagctgctc gcgaagttgc cgaagatcca ccggccccct gctccctggc cccctgctcc
     4201 ctggccccgc gctccctggt gcagccctat ctggtccaac gccgtgcgca tcgtccgggg
     4261 tcagatccca gccaagatcg atcgcgccgg tgtcgtctct tgtgacagag acctggccat
     4321 gatccacttc gatcgtccgc gggacgatac ggcccagcag gatgccggcg aaggagagtg
     4381 tcaggtgggc ttccggagcg gcgatcagtc gccggcctgc cgagtcggta atgctgatat
     4441 tggagatctg gagatcgagc gggtaatcga cgcctttatg aaaaccctcc catgccagcg
     4501 cgaggcggtc gaacgacacc cgaattggtg ccgtgtcatc gaccagttca gccttcacgc
     4561 gatccgcgag ccagccaagg tcgatcggcc cttgcgccag ccgccatgcg gccccggcca
     4621 gcaggcagct cgcgacgacc aggagcccgg ttccgacgat cagcagccgg tgtaccgctc
     4681 tggaggtgtg gtggatggcc catcgcgtgt ggcggaccgc cgcgtggctt tttgagcgat
     4741 ggaaccagcc accttcctga tgccaggcca atggccgacc ccttacgacg aagccgccgc
     4801 ggatcggctt gtcgagcgct tccgaacgct cggcgaaacc gaaagccggc tgttggaggc
     4861 ggcaggcgtc ctacccatgc ttcgatgtct cggcggcaat agtccctacc tcgctgcatt
     4921 ggctttgcgg gaacctgaat cgatccacga tctggcaacg catggaccct ccgccgtgtt
     4981 cgcgcatggc ctgtcccgcc tcagcaatct ttccccggcc gccaagcgcc ccgccgtcgc
     5041 cgcgggtctt cggcaggcga aaa
//
LOCUS       DASWQN010001331         3573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_263578, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001331 DASWQN010000000
VERSION     DASWQN010001331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263578"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VGC82_22575"
     CDS             <1..1154
                     /locus_tag="VGC82_22575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY0426119.1"
                     /translation="LAQQKRSSARSAHRGKDAGIPTGSPADTPLGSVDTAAKWAYIQD
                     FETGATLLEKHADEEMPPSSMTKLMTVYIVYDRLKQGRLKLEDELPVTEKAWRTQGSK
                     TFVQIGTSVKVEDLIRGVIVQSGNDAAIVLAEAIAGSEEQFAEQMNAKAKELGLTHSF
                     FKNCTGWPDPEQHMSCRDIATLAARFIQDFPEYYHYDAEKSFRYNGIEQGNRNPMVQK
                     GTADGLKTGHTDAGGYGLVASSLRNGRRVILVLNGMTSMHERAEEAERLMDWAFFNFE
                     DVTLFAAGDVVESVPVWLGTTRTVPLVAGRNVVVTMPRNWRQKASVKVSYASPLAAPV
                     AKGDSLGKLTVTGDGVPNLSVPLMAAADVPLLGLPGRAMAVLSKYVTGT"
     gene            1151..1783
                     /gene="tmk"
                     /locus_tag="VGC82_22580"
     CDS             1151..1783
                     /gene="tmk"
                     /locus_tag="VGC82_22580"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY0426120.1"
                     /translation="MTPARGRFITLEGGEGTGKTTQSDLLSEALVARGITVVRTREPG
                     GSPGAELLRGILLDGTVDWSPRAETLLHFAARAEHVAKIIQPALDTGSWVVCDRFYDS
                     TLAYQGYGQGADLGVIHEQIRLLGMRPDLTLVLDLEEAVAAGRRRIRGERADRYERLD
                     AAFHARVRRGFREIAAKVPDRCVLLDASGDVQSVHASIMTAVWTRLGWDP"
     gene            1902..2132
                     /locus_tag="VGC82_22585"
     CDS             1902..2132
                     /locus_tag="VGC82_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEY0426121.1"
                     /translation="MLHRYTPEVDIRTNIEIDDGLIGEAMRAIGQTTKRGTVEEALRR
                     VVINHRRQQAIADMAGLGWDGDLQAMRTGQTD"
     gene            2129..2518
                     /locus_tag="VGC82_22590"
     CDS             2129..2518
                     /locus_tag="VGC82_22590"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0426122.1"
                     /translation="MIVVDTSVWIANLRDQPLAVVRRLREPAITDDILLGDIVLTEVL
                     QGARNDTHAAALENAFRQFPIVAMVGGAIAAKAAYNYRKLRGAGFTMNKIAALFIGTY
                     CIEHGHHLLQCDADFQPMTSVCGLRLH"
     gene            complement(2593..>3573)
                     /gene="tcuA"
                     /locus_tag="VGC82_22595"
     CDS             complement(2593..>3573)
                     /gene="tcuA"
                     /locus_tag="VGC82_22595"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEY0426123.1"
                     /translation="IPVLYDTTAYQLLTDDARIQGEKVRHGGRPYDLRAKSVVLACGG
                     FESNAEMRARYIGPNWDLAKVRGTRFNNGYGHKMAMDIGAASAGHWSGAHAVQWDMNA
                     PPYGDISIGDRFQKHNYPFGVILNARGERFLDEGLDFHSYTYAKYGHEVMKQPGLFAW
                     QVVDQKIINLLRDEYRIARITKETASTMEELVVKLTGVDPAAALATLKAYNDAPRPDT
                     PFNPNIHDGLRTCGLPIDKTNWAQRLDTPPYHAYGVTAGVTFTFGGLKVSTQAEVQDT
                     SGVAINGLFAAGEIVGGLYYHNYGSGTGLVAGVTFGRIAGDGAARFASGR"
BASE COUNT          663 a         1047 c         1173 g          690 t
ORIGIN      
        1 gcttggccca gcagaaacgt tcgtccgcgc gttccgccca caggggcaag gacgccggca
       61 ttcccaccgg aagcccggct gacacgccgc tgggatccgt cgataccgcc gcgaaatggg
      121 cctatatcca ggacttcgaa accggcgcca ccttgctgga aaagcacgcg gacgaggaga
      181 tgccgccgtc gtcgatgacc aagctgatga cggtgtacat cgtttatgac cggctgaagc
      241 aaggccggtt gaagctggaa gacgaactgc cggtcaccga gaaagcctgg cggacccagg
      301 gttcgaagac gttcgtgcag atcggcacct ctgtcaaagt cgaagacctg atccggggcg
      361 tgatcgtgca gtccggcaac gacgcggcga tcgtgctggc cgaggcgatc gccggttcgg
      421 aagagcagtt tgccgaacag atgaacgcga aggcgaagga acttgggtta acccactcat
      481 tttttaaaaa ctgcaccggc tggcctgatc ctgaacagca catgagctgc cgcgacatcg
      541 cgaccctcgc ggctcggttt atccaggatt ttccggaata ctatcactac gacgccgaga
      601 aatcgttccg ctacaatggc atcgagcagg gcaaccgcaa tccgatggtg cagaaaggca
      661 cggcggacgg cctgaagacg ggtcacaccg atgccggtgg atacggtttg gtcgcaagtt
      721 cattacgtaa cgggcggcgg gtgatcctgg ttctgaacgg catgacctcg atgcatgagc
      781 gggccgagga agccgaacgg ctgatggact gggcgttctt caatttcgag gacgtgacgc
      841 tgttcgccgc cggcgacgtg gtcgagagcg ttccggtctg gttaggcacc acgcggacgg
      901 tgccgctggt agccggccgc aacgtcgtgg tgacgatgcc acgtaactgg cggcagaaag
      961 cctcggtcaa ggtcagctat gccagcccgc tggcagcccc ggttgccaaa ggcgactccc
     1021 tgggcaaact gaccgtgacc ggcgacggcg tgccaaacct gagcgtgccg ctgatggcgg
     1081 cggccgatgt gccgctgctt gggttgccgg gccgcgcgat ggcggtgctt tcgaaatacg
     1141 tgaccggcac gtgacgcctg cccgcggtcg cttcatcacc ctggaaggcg gcgaaggaac
     1201 aggcaagacg acacaatccg acctgttgtc cgaagctctg gtggcacgcg gcatcacggt
     1261 cgttcggacc cgcgaaccgg gcggctcgcc gggtgccgag ctgctgcggg gaatcctgct
     1321 ggacggtacg gttgactggt cgccgcgggc cgaaacgctg ctgcatttcg cagcgcgcgc
     1381 ggagcacgtg gcaaagatca tccaaccggc gctggacacc ggaagctggg tggtctgcga
     1441 ccggttctac gattcgacgc tggcctatca gggatacggg caaggcgccg acctgggtgt
     1501 catccatgaa caaatacggc tgctgggaat gcggcccgat ctgacgttgg tactggatct
     1561 ggaggaggct gtcgccgccg gccgccggcg gatccgtggc gagcgtgccg accgctacga
     1621 gcgcctggat gcggcgtttc acgctcgggt acgacggggt tttcgggaga tcgcggccaa
     1681 ggtgcctgat cgttgtgtct tgctggatgc ctcgggcgat gtccagtcgg ttcatgcttc
     1741 catcatgaca gcggtttgga cgcggctggg ttgggacccg tagctgagtt cgatggacga
     1801 agattggggc tctctcgtcc gaacggccgt cgctcggggt tgcgcgctcc aggcttcgtc
     1861 cagcttgttc cactgcgctc tggttgaatg atgtgtattg tatgctacat cgatacacac
     1921 cggaggtcga tatacgaacg aacattgaga tcgacgacgg cttgattggc gaagccatgc
     1981 gggcgatcgg acagacgacc aagcgcggaa ccgtcgagga agccctgcgc cgggtggtga
     2041 tcaatcatcg tcgccagcaa gcgattgccg acatggcagg gctggggtgg gacggcgatc
     2101 tgcaagcgat gcggaccggc cagaccgatt gatcgtcgtc gatacgtcgg tctggattgc
     2161 caacctgcgc gatcaacctt tggccgtcgt ccgaaggttg cgggagccgg cgatcacgga
     2221 cgacatcctt ctcggcgata tcgtcctgac cgaggtgttg caaggcgctc ggaacgacac
     2281 ccacgctgcc gctcttgaaa acgccttcag gcagttcccg atcgtcgcga tggttggcgg
     2341 tgcgatcgcc gccaaggcgg catataatta ccgcaagctg cgcggcgccg gcttcaccat
     2401 gaacaagatc gccgctctgt tcatcggaac gtattgtatc gagcatgggc atcacctgct
     2461 gcaatgcgac gccgacttcc agccaatgac ttcggtttgc gggctgcgat tgcattgacg
     2521 acaaatgtcg tcaatgcagc gagatgcagc ttcgggagcg ccgcgatatc tcgctcatgt
     2581 gtgtctcggc cattatcggc cgcttgcgaa ccgtgccgcg ccatccccgg cgatgcgccc
     2641 gaaggtcacg ccggcgacca ggcccgtgcc actgccgtag ttatgatagt ataggccgcc
     2701 gacgatctcc cccgccgcga acaggccgtt gatcgcaacg ccggacgtat cctgcacctc
     2761 ggcctgcgtg ctgaccttca ggccgccgaa ggtgaaggtg acgccggcgg tcacgccata
     2821 ggcgtgatag ggtggggtat ccagcctctg cgcccagttg gtcttgtcga tcggcaggcc
     2881 gcatgtgcgc aggccatcat ggatattcgg attgaacggc gtgtccggcc gcggcgcatc
     2941 gttgtaggcc ttcagcgttg ccagcgcggc ggccgggtcg acgccggtca atttaactac
     3001 gagttcttcc attgtactag ccgtttcttt ggtaatccga gcgatgcgat attcgtcgcg
     3061 tagcaggttg atgatttttt ggtcgactac ttgccaggcg aataatccgg gctgcttcat
     3121 tacctcgtgg ccgtatttgg catacgtgta gctgtggaaa tccagacctt catccaggaa
     3181 ccgttcgccg cgggcgttca ggatgacacc aaacggatag ttgtgtttct ggaagcggtc
     3241 gccgatggag atgtcgccgt aaggcggagc gttcatgtcc cactgcaccg cgtgcgcccc
     3301 cgaccagtgc ccagcggagg cggcgccgat gtccatcgcc atcttgtggc cgtagccgtt
     3361 gttgaagcgg gtgccgcgga cttttgcaag atcccagttc gggccgatat agcgggcgcg
     3421 catctcggca ttggattcga agccgccgca ggccagcacg acggatttgg cccgcagatc
     3481 gtatggccgg ccgccatggc gcaccttctc gccctggatg cgggcgtcat cggtcagcag
     3541 ttgataagcg gtcgtgtcat acagcactgg gat
//
LOCUS       DASWQN010001332         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1978571, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001332 DASWQN010000000
VERSION     DASWQN010001332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978571"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VGC82_22600"
     CDS             complement(<1..210)
                     /locus_tag="VGC82_22600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426124.1"
                     /translation="MTAFLVVRAVVADPADRPPFDHWYRTEHLPDALKAFGAVAAMRA
                     WSTVDPTVHTAFYRFNALEDVQAISG"
     gene            complement(207..1136)
                     /locus_tag="VGC82_22605"
     CDS             complement(207..1136)
                     /locus_tag="VGC82_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0426125.1"
                     /translation="MKLAFFDDFKLGVVKGDAIHDVSAVVKDIPHVGPGDLINSLIAN
                     FADYRTKLEAAASGTGTKLASVRIRPPLPKPGNIVCMAVNYMENGTLAKPAPINAFHK
                     APTAIIGPGDTMVLPDVPATIFEGEAEMAVVIGKRAHQVPAAQAMDYIFGYINFIDGS
                     ARGLPPAGNVFFQMKSRDTFAPIGPYLVTKDEIEDPQKLPINLWVNGELKQSFNTDDM
                     AHNIARCIEWVTSIHTLEAGDILATGTNHRGLSSFMDGDTVELETQGLGKLSFKIRDD
                     LKRTWSRETRLDRQKKNQEGPTHQLTGKYANPA"
     gene            1267..1579
                     /gene="ssrA"
                     /locus_tag="VGC82_22610"
     tmRNA           1267..1579
                     /gene="ssrA"
                     /locus_tag="VGC82_22610"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            complement(1587..2282)
                     /locus_tag="VGC82_22615"
     CDS             complement(1587..2282)
                     /locus_tag="VGC82_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0426126.1"
                     /translation="MSDITRMASVAALLGDPARANILTALMDGRALTAKELAFAAHVS
                     PQTASGHLARLTEAHLLEVEKQGRHRYFRLASPLVGQMLESVMAVAGPEPGPINRWRG
                     GEALRMARTCYDHLAGRLGVALTDSLIEAGHITLSPDGGEVTECGHAFLAHFGAEPAA
                     GKRVFCRPCLDWSERRPHIAGRLGAALAMRCLELGWIQRQRDSRAVTITAAGALGFAD
                     KFGVQLPHCQQSH"
     gene            2416..>2654
                     /locus_tag="VGC82_22620"
     CDS             2416..>2654
                     /locus_tag="VGC82_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426127.1"
                     /translation="MNDLFGSSPQKSTPKPPAKPPSSQVKPPSSQVKAETERRLPLPV
                     RDDSDYSAKDIEVLEGLEPVRKRPGMYIGGTDEAA"
BASE COUNT          485 a          804 c          856 g          509 t
ORIGIN      
        1 cccgctaatc gcctgcacgt cttccagcgc attgaagcgg taaaacgcag tatgtaccgt
       61 cggatcgacg gtactccagg cacgcatcgc cgccactgcg ccgaacgcct ttagcgcgtc
      121 cggcagatgt tcggttcgat accagtggtc gaacggcggg cggtccgccg ggtcggcgac
      181 gaccgcgcga accacaagga atgccgtcat gccggattcg catatttgcc ggtaagctga
      241 tgcgtcgggc cctcctggtt cttcttctgc cggtcgaggc gggtttcgcg cgaccacgtg
      301 cgcttgaggt catcacggat cttgaagctc agcttgccga ggccctgggt ttccagttca
      361 accgtgtcgc cgtccatgaa gctggacagg ccacgatggt tggtgccggt tgccaggatg
      421 tcgcccgctt ccagcgtatg gatcgaggtc acccactcga tgcagcgcgc gatgttatgg
      481 gccatgtcgt cggtgttgaa ggattgtttc agctcgccgt tgacccaaag gttgatcggc
      541 agcttctgcg ggtcctcgat ctcgtccttg gtcaccaggt acggcccgat gggtgcgaag
      601 gtatcgcgcg acttcatctg gaagaacacg ttgccggcag gtggcaggcc acgggcggag
      661 ccgtcgatga agttgatata gccgaaaatg tagtccatcg cctgcgctgc cggaacctgg
      721 tgggctcgtt tgccgatgac gacggccatt tcggcctcgc cttcgaagat cgtcgcgggt
      781 acgtccggga gcaccatggt atcgcccggc ccgataatgg ccgtcggggc cttgtggaag
      841 gcattgattg gcgcgggctt cgccaacgtg ccgttttcca tgtaattgac cgccatgcag
      901 acgatgttgc cgggcttggg cagcggcgga cggatgcgga cgctggccag cttcgtgccg
      961 gtgccgctcg cggcggcttc aagcttggtc cggtaatcgg cgaagttggc gatcaggctg
     1021 ttgatcaggt cgccgggtcc gacatggggg atatccttca ccacagccga aacatcatgg
     1081 atcgcgtcgc ctttgacgac gcccagcttg aagtcatcga aaaatgccag cttcatggcg
     1141 gatcctcccg aggattgttg tgtcgggcga tcatagagca ttccgcgtgg gctgggaacg
     1201 caggatgccg gatcaccgat cgggcccgaa ccttgcgatc ggggccctgc gacaggccct
     1261 gcgatcgggc ttgaagcggc agggcaatta gcttagatac attccgtcag cttgctgact
     1321 atggcgataa acggtgggtg aaataatcgt tgtggacccg ggggcagtgc ccggcgcctc
     1381 caccatccgt cccgggttat tgggacatag gggggcgaaa caggctcgac acgcgtggta
     1441 aagacacatc ttttgcccgg catgataccg ccgttatcgg gtcaaatcac aagtgccaat
     1501 gataacgagg cgctcgccgt cgctgcataa gcgataggcg cggttcgggg gggcaccggg
     1561 caacagaagc ccccccaatt cttctttcag tgcgattgct gacagtgcgg tagctggacg
     1621 ccgaatttat cggcaaaacc gagcgccccg gccgcggtga tggttaccgc gcgggagtcg
     1681 cgctggcgtt ggatccagcc cagttccagg catcgcattg ccagcgccgc gcccagccgg
     1741 ccggcgatgt gcgggcgtcg ctcgctccaa tccaggcagg ggcggcagaa gacgcgcttt
     1801 cctgccgccg gttcggcgcc aaaatgtgcc aggaacgcat ggccgcactc ggtgacctcc
     1861 ccaccgtccg gtgataacgt gatgtgaccg gcttcaatca acgaatcggt caacgcgaca
     1921 ccgagccggc ccgcgaggtg gtcgtaacag gtgcgggcca tgcgcagcgc ctcgccaccg
     1981 cgccagcggt taatcggtcc gggttcagga ccggcgactg ccatgacgct ttccagcatc
     2041 tgcccgacca gcggcgaggc caaccgaaaa taccggtggc gtccctgttt ctcgacttcc
     2101 aacaggtgcg cctcggtcag gcgggccagg tgtccgctgg ctgtctgcgg cgagacatgg
     2161 gccgcgaaag ccagttcctt cgcggtcagc gccctgccgt ccatcaatgc cgtcaggata
     2221 ttggctcggg ccggatcacc cagcagcgcg gcgaccgagg ccatgcgtgt gatgtcgctc
     2281 atggagcgaa acatagaccg gatcggcgtc cagcgcatgc gacgatagtt cggcggaagc
     2341 cgaattgttc tttcaacact gttgccgtga catgccgtcg ccatgtaggg tgccattcag
     2401 cactgggaac acagcatgaa cgacctgttt ggatccagcc cccaaaaatc gaccccgaag
     2461 ccgcctgcca agccaccttc ttcgcaggtg aagccacctt cttcccaggt gaaggcggag
     2521 acggagaggc ggctgccatt gccggtgcgt gacgactcgg actactccgc caaggacatc
     2581 gaggttctgg aagggctgga gccggttcgc aaacggcccg gcatgtatat cggcggcacc
     2641 gacgaagcgg cctt
//
LOCUS       DASWQN010001333         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2374107, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001333 DASWQN010000000
VERSION     DASWQN010001333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2374107"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VGC82_22625"
     CDS             complement(<1..708)
                     /locus_tag="VGC82_22625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY0426128.1"
                     /translation="MLLQHQTWQEVEAYLERSKAIILPIGSTEQHGPNGLIGTDAICA
                     EVIARGVGEAAGAMVGPTIGVGIAVHHMGFAGSMTVRPSTLIAIMHDYVLSLARHGFR
                     RFFFINGHGGNIATVQAGFSEIYATFEGAIGVRRDDELKCRLANWWDGKVMHELGKEV
                     FGDDEGSHATASEVAVTQYVLPETVKQAQLDPPRAPTGRFHGPADYRRRFPDGRIGSN
                     PGLATPEKGKRFYDAAIP"
     gene            821..1408
                     /locus_tag="VGC82_22630"
     CDS             821..1408
                     /locus_tag="VGC82_22630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426129.1"
                     /translation="MEMATIGDSKGEFRGLSYRNDADLKAWLAKRPTESALEPALPII
                     DPHHHFWDTPQRGRYFLPDLLADIGGGHNIVSTVFMECQAMYRKDGPAEMAPVGEVEF
                     VNGIAAMSASGTYGPCRVAEVIIGHADLRRGARVRDVLEAEIAVGGGRFRGIRYGLSW
                     DADPSISRFASRIVPPHLARDATFREVSLFLRNWD"
BASE COUNT          293 a          529 c          492 g          309 t
ORIGIN      
        1 cggtatggct gcgtcgtaga atcgcttgcc cttttccggc gtggcaagcc cagggttgga
       61 accgatgcga ccatccggga agcgccgccg gtaatccgcc gggccatgga accgaccggt
      121 cggcgcgcgc ggcggatcga gctgtgcttg tttgaccgtt tccggcagca cgtattgggt
      181 caccgccacc tcgctggccg ttgcgtggct gccctcgtcg tcgccgaaca cttcttttcc
      241 cagctcatgc atcaccttcc cgtcccacca gttcgccagg cggcacttca attcgtcatc
      301 ccgccgaacg ccgatcgcgc cttcgaatgt ggcatagatc tcggagaaac ctgcctgaac
      361 ggtggcgatg ttgccgccat ggccattgat gaaaaagaat cgccggaacc cgtggcgcgc
      421 gagggacagg acataatcgt gcatgatcgc gatcagcgtc gacggccgca ccgtcatcga
      481 tccggcaaat cccatgtggt ggaccgcgat accgaccccg atcgtgggac caaccatggc
      541 accggccgcc tcgcccacgc cgcgggcgat cacctccgca cagatcgcgt ccgtcccgat
      601 cagcccgttc gggccatgct gctcagtcga tccgatcggc aagatgattg cttttgatcg
      661 ctcgaggtaa gcctcgactt cctgccatgt ctggtgttgc aaaagcatgt gggatgatcc
      721 tcctgataga ctattctacc gcagcggccg gaaagacgcg aacctggcgt cgttgacggt
      781 aatgtccagg cgccctcatg ctgtgttcaa cgaacggggg atggaaatgg cgaccattgg
      841 cgactcgaag ggcgaattcc gcggtctcag ctatcgcaac gatgccgacc tgaaggcatg
      901 gctggcgaag cgcccgaccg agtcggcctt ggaaccggcc ttgccgatca tcgatccaca
      961 tcaccatttc tgggacacgc cgcaacgcgg gcggtacttt ctgccggatc tgctcgccga
     1021 tatcggcggc ggacacaaca tcgtctccac cgtgttcatg gaatgccaag ccatgtaccg
     1081 caaggacggt ccggcggaga tggcaccggt cggcgaggtc gaattcgtca acggcatagc
     1141 cgccatgagc gcatccggca cttacggccc gtgccgcgtc gccgaggtaa ttatcgggca
     1201 cgccgaccta cgccgggggg ctcgggtgcg cgacgtgctg gaggccgaaa tcgccgtggg
     1261 cggtggccgc tttcgcggca tacgctacgg cctatcgtgg gacgccgatc catcgatcag
     1321 caggttcgcc tcccgtatcg tgccgccgca cctggctcgc gacgccacgt tccgcgaagt
     1381 ttcgctgttc ttgcgaaact gggactgagt ttcgaatcgt ggcagtacca tccgcaactt
     1441 tcggacgcga tcgacctggc ccgcgccttt cccgacacga ccatcattct gaatcatgtc
     1501 ggcggcgcgc tgggcgtcgg accctattcc ggtcgccgcc aggaatcctt gccggctggc
     1561 agaaggacat caaggagttc gccaagtgcc ctaacgtcaa cgtcaagctc ggtggcgtcg
     1621 gca
//
LOCUS       DASWQN010001334         3666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1186437, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001334 DASWQN010000000
VERSION     DASWQN010001334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186437"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /gene="glgX"
                     /locus_tag="VGC82_22635"
     CDS             complement(<1..1254)
                     /gene="glgX"
                     /locus_tag="VGC82_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY0426130.1"
                     /translation="MPPAILKSRVSEGFSHQLGATWDGLGVNFALFSANATRVELCLF
                     DDAGERELERIELPEYTNEVWHGYLPDARPGTTYGYRVHGPYEPDFGHRFNPNKLCLD
                     PYAKAHVGSLNWDPAIFGYTMGEEDTTFDERDSARFVPKSRVIDPAFTWGRERAPSVP
                     WDRTIIYETHVRGYTMQHPAVPERLRGTFAGLGRKEVVDYIKTLGVTSIELLPVHAFV
                     NDSYLLDKGLTNYWGYNSIGFFAPDPRYSAVPDFVFSEFKEMVARLHDAGLEVILDVV
                     YNHTAEGNENGPTLSFRGIDNMSYYRLMPEQRYYINDTGTGNTLNMSHARVVQMVTDS
                     LRYWAGEMHVDGFRFDLGTILAREADGFNEAHGFLQSCLQDPVLSATKLIAEPWDIGP
                     GGYQVGGFPPGWAEWNDKFRDTVRAY"
     gene            complement(1359..1589)
                     /locus_tag="VGC82_22640"
     CDS             complement(1359..1589)
                     /locus_tag="VGC82_22640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY0426131.1"
                     /translation="MSERVEGTAREFGGRVQEAAGNLTGDTRNQAEGLYNQASGQAQQ
                     AAAQFSDVVKAQPVVASLVALAIGYVLGRLTA"
     gene            1696..2403
                     /locus_tag="VGC82_22645"
     CDS             1696..2403
                     /locus_tag="VGC82_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY0426132.1"
                     /translation="MRTFDLASENWSASALADEMAAEGYATRILLPPQACQALAALYD
                     QDDRFRKRIVMEQHGYGRGEYKYFGYPLPDPVARLRTTLYPPLAQVANAWRRTLGEPE
                     AFPDALEDYLAQCRAAGQNRPTPLLLRYGAGGFNCLHQDLYGDLVFPLQVTVLLNAPG
                     EDFTGGEFIMVEQRPRAQSRPIVVPLRQGEAVIFPVHHRPGMGTRGPYRLTMRHGVSR
                     INSGRRHTLGIIFHDAA"
     gene            complement(2439..3044)
                     /locus_tag="VGC82_22650"
     CDS             complement(2439..3044)
                     /locus_tag="VGC82_22650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0426133.1"
                     /translation="MNSTPKSNRILYATMGLLLAILLIGAGGFLWLGGSGGNNPIGIG
                     GPFVLQDGDGKPVTDKDFRGRYMLVYFGYTFCPDVCPTTLNAVADAMDRLGPASARIQ
                     PLFITVDPKRDTPAVVKQYVAAFGSSIRGLTGTPEEIAKVAKEYRVYYVEHPAESGAT
                     DYSVDHSSVLYLMDPKGGFLAPVRADQSGAEIAANLKKLTG"
     gene            complement(3065..>3666)
                     /locus_tag="VGC82_22655"
     CDS             complement(3065..>3666)
                     /locus_tag="VGC82_22655"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY0426134.1"
                     /translation="TGFAQGLELIGGMPGLFGTPYGHIALLKIALFLLALVLAAVNRL
                     WLTDRLSDETTGAKRHLSMSVCVETIIGLTIVTAAASLASVVPGVHQTPVWPLSWPFS
                     LGTVNQDAQFHQQFAFSLITVGAAVALLAAALLARRFRLLATAVLAATVLWRGPTLSP
                     LPIRAAPTTSLTAPVRSPASFLVGVIASTADRQHLRPSY"
BASE COUNT          701 a         1078 c         1177 g          710 t
ORIGIN      
        1 gtacgcccgg acggtgtcgc ggaacttgtc gttccattcc gcccaacccg gcgggaagcc
       61 gccgacctga tagccgccgg gtccgatatc ccagggttcg gcgatcagct tggtggccga
      121 cagtacgggg tcctgcaggc agctctgcaa aaagccgtgc gcttcgttga atccgtcggc
      181 ttcgcgcgcc aggatcgtgc cgagatcgaa ccggaagccg tcgacgtgca tttcgccagc
      241 ccaatagcgc aggctgtcgg taaccatctg cacgacgcgg gcgtggctca tgttgagcgt
      301 gtttccggtg ccggtatcat tgatgtagta gcgctgttca ggcatcaacc ggtagtacga
      361 catattgtcg atgccgcgga aagacagggt cgggccattc tcgttgcctt ccgccgtgtg
      421 gttgtaaacg acgtccagga tgacttccag gccggcgtcg tgcaggcggg cgaccatctc
      481 cttgaattcc gagaacacaa agtcgggcac ggccgaatag cgcggatcgg gggcaaagaa
      541 gccgatcgag ttgtagcccc agtagttggt cagccctttg tccagcaggt agctgtcatt
      601 aacgaaggcg tggaccggca gcagctcgat cgaggtgacg ccaagggtct tgatgtagtc
      661 cacgacctcc ttgcgcccca ggccggcgaa tgtgccgcgc aatctttccg gcaccgccgg
      721 atgctgcatc gtatagccgc ggacatgggt ttcataaatg atggtgcggt cccagggaac
      781 cgatggcgcg cgttcccgtc cccaggtgaa tgccgggtcg atgacgcggg atttcggcac
      841 gaagcgggca ctgtcgcgtt catcgaacgt ggtgtcttcc tcgcccatcg tgtagccaaa
      901 aatcgccgga tcccagttca ggctgccgac atgcgccttg gcgtaagggt ccaggcacag
      961 tttgtttggg ttgaagcgat ggccgaaatc cggctcgtag gggccatgaa cgcgataacc
     1021 gtaggtcgtg ccggggcggg catcgggcag gtatccgtgc cagacttcat tggtgtattc
     1081 gggtagttcg atcctttcga gctccctttc accggcatcg tcgaacaggc aaagctcgac
     1141 tctcgtggca ttcgcagaaa acagcgcgaa attgacgccg agcccgtccc atgtcgcgcc
     1201 cagctgatga gaaaatcctt cactgacgcg cgacttcagg atggcagggg gcatttttga
     1261 acctcttcac ggtgcaagtg acaaggggaa gttgggggca acgtcagccg ggcaaaaaaa
     1321 agggggcgca ctgccccctc tttctcacgt cgattgcgtc aggccgtcag acgaccaagg
     1381 acgtaaccaa tggccagcgc caccagcgaa gcgacaacgg gctgtgcttt gaccacatcg
     1441 ctgaattgtg cggcggcctg ctgcgcctgc ccactcgctt gattatacaa gccctccgct
     1501 tggttcctgg tatcgcctgt caggttcccg gcggcttcct gcacgcgacc cccaaattcg
     1561 cgggccgtgc cttcgacgcg ttcgctcatg ctcgtctcct gtcaaatctg gagtgatatt
     1621 aacgcacgcg gcgggttttg gttgcaaagc accaaccggg atggcggacg gcggaaagcg
     1681 ggatagggta gggtgatgcg aacatttgat cttgcttccg aaaattggtc cgcgagcgcg
     1741 ctggctgacg aaatggccgc cgagggttac gcgacccgga tcttgctccc gccgcaagcg
     1801 tgccaggcgc tggccgcgct ttacgaccag gacgatcgct tccggaagcg tatcgtgatg
     1861 gagcaacacg gctacggccg aggggaatac aaatatttcg gctatccgct gccggatccg
     1921 gtcgccaggt tgcggaccac attgtatccg ccgcttgctc aagtggcgaa tgcctggcgc
     1981 cggacgttgg gcgaacccga ggcgtttcct gacgcactgg aagattatct ggcgcaatgc
     2041 cgtgccgcag ggcaaaaccg tccgacgcct ttgttgctgc gctatggggc gggcggcttc
     2101 aactgcctgc accaggattt gtatggggat ctggttttcc cgttgcaggt cacggtgttg
     2161 ctcaatgcgc cgggcgagga tttcaccgga ggcgagttca tcatggtgga gcaacggcct
     2221 cgcgcgcagt cccggcctat cgttgtgccg ttacggcagg gcgaagcagt gatctttccg
     2281 gtgcatcacc gaccggggat gggtacgcgc gggccttata ggctgacgat gcggcacggt
     2341 gtcagccgga tcaactccgg ccggcggcac accctgggga ttatcttcca tgacgcggct
     2401 taaacgcgaa tgactgcggg atctgtcctg tcggacggtc accccgtcag ttttttcaga
     2461 ttggcggcaa tttcggctcc ggactgatct gcccgcaccg gcgccagaaa gccgcctttc
     2521 ggatccatca gataaagcac cgaactatgg tcaaccgagt agtccgtggc gcctgactcg
     2581 gcggggtgtt caacataata gacccgatat tcctttgcga ccttcgcgat ttcttctggc
     2641 gtcccggtga gtccccggat gctggacccg aacgcggcca cgtactgctt caccaccgcc
     2701 ggcgtgtccc gctttggatc gaccgtgatg aacagcggct gaatcctggc cgaggcggga
     2761 ccgagcctgt ccatcgcatc ggcgacggcg ttgagcgtgg tggggcagac gtccgggcag
     2821 aaggtatagc cgaagtaaac cagcatatat ctgccccgga agtccttgtc ggtgacgggc
     2881 ttgccgtcgc cgtcctgaag tacgaagggc ccaccgatgc cgatcggatt gttgccgccg
     2941 ctgccgccca gccacaggaa accgccggcg ccgatcaaca ggatcgccag cagcagtccc
     3001 atcgttgcat acaggatccg gtttgacttt ggggtagagt tcatgcggtt ctccgctgaa
     3061 ttgttcaata ggatggtcgc agatgctgcc gatcagcggt ggatgcgatg acgccgacaa
     3121 ggaaggaggc aggggatcga actggagcgg tcagcgaggt cgtcggagcg gccctgatgg
     3181 ggagcggtga cagggtgggg ccacgccaca ggacggtggc cgccaggacg gccgtcgcca
     3241 acagacggaa ccggcgcgcc aggagcgctg cggccaataa tgccaccgct gctccgactg
     3301 tgatgaggct gaaggcgaat tgctgatgga attgtgcatc ctggtttacc gtgccgaggc
     3361 tgaacggcca cgatagcggc cagaccggcg tctggtgtac gccgggcacg accgatgcca
     3421 aggatgccgc cgcggtaacg attgtcaggc cgatgatggt ctcgacacag acagacatcg
     3481 aaagatgccg cttcgccccg gtcgtttcgt ccgacagccg gtcggtgagc cagagccggt
     3541 tcaccgcagc cagcaccaaa gcgagcagga acagggcgat tttcagcaag gcgatgtgcc
     3601 catacggcgt accgaagagg cccggcattc cgccgatcag ctccaaccct tgagcaaagc
     3661 cggtgc
//
LOCUS       DASWQN010001335         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1186857, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001335 DASWQN010000000
VERSION     DASWQN010001335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186857"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..424)
                     /locus_tag="VGC82_22660"
     CDS             complement(188..424)
                     /locus_tag="VGC82_22660"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0426135.1"
                     /translation="MTELKLAGRPADLFDLGNTLLERGQAGAAIDAFKRSLALAPNHA
                     ASLYNLGNALLQAGTPQVSVAHDMSGLGSTRSVR"
     gene            complement(528..>1859)
                     /locus_tag="VGC82_22665"
     CDS             complement(528..>1859)
                     /locus_tag="VGC82_22665"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0426136.1"
                     /translation="LADDMGLGKTVQTLAHLMIDKAAGRLDRPSLIVCPTSLIPNWTA
                     EARRFAPELSVLPLHGPARKANFPAIPKHDLVLSTYPLLSRDHEVLQKQKWHAVILDE
                     AQSIKNPNAETTRQALRLQARQRLCLSGTPLQNHLGELWSLFDFLAPGFLGGQKSFKS
                     RYRTPIEKHGDVERQELLTRRIRPFMLRRTKQEVISELPPKTEIVEPVEMETTQRAIY
                     EAIRLSMHAKVQAAIAQKGLARSGIIILDALLKMRQACCDPRLLKLKTVAKSKAGSAK
                     LDRLMEMLSIMFAEGRRVLLFSQFTEMLALIETRLTDDGVDFVKLTGDTKDRGTPVKK
                     FQAGDVPLFLISLKAGGVGLNLTAADTVIHYDPWWNPAVEDQATDRAHRIGQTKKVFV
                     HRLVTLGTIEEKMEVLKDKKRAIVASVMDAEHGGALTMTEADVEELFAPAG"
BASE COUNT          329 a          591 c          551 g          388 t
ORIGIN      
        1 ccgtccgcct cgaagcgaac gcaccaacgg cggaacgtcc gttcggtgat gcccaacaac
       61 tcagccgctt caatctggtt gaggtcgctc gattcatagc ggcccaagac actccggaat
      121 ttgatcactc gcacaccctg aagagcttcc gtccagcgca tctgggctct ctccaaagag
      181 cccaatatca ccggacagat cgtgtgctac ctaaaccgga catatcgtgt gctaccgaca
      241 cttgcggggt tccggcctgg agcagcgcat tgccgagatt ataaagactc gccgcatgat
      301 ttggcgcgag ggccagcgac cgcttgaagg cgtcgatcgc cgcgccggcc tgtccacgtt
      361 cgagcagcgt gtttcccaag tcgaataagt ccgccggacg acccgccagt ttgagctctg
      421 tcatccacgc tcaccgtcac tgtccatcag ccatggtatc tggccgagga ttaacgactc
      481 gttaacgatg atgatgatga tcgcattgtc ggcaatcgcg ccggcagtca cccggcgggg
      541 gcgaacagct cttccacgtc ggcctcggtc atggtcagcg cgccgccatg ctccgcgtcc
      601 atgacactgg cgacgatggc gcgtttcttg tccttcagaa cctccatctt ctcctcgatc
      661 gtcccgagcg tcaccaagcg atgaacgaag actttcttcg tctgcccgat ccggtgtgcc
      721 cgatcggtcg cctggtcctc caccgccgga ttccaccaag gatcgtagtg aatgaccgtg
      781 tcggcggcgg tcaggttcaa cccgacgccg ccggccttca ggctgatcag gaacagcggc
      841 acgtcgccgg cctggaattt tttcaccggc gttccccggt ccttggtatc gccggtcagc
      901 ttgacgaaat cgacgccatc gtccgtcagc ctggtttcga tcaatgccag catctcggtg
      961 aactgcgaga acagcagcac gcgccgccct tcggcgaaca tgatgctgag catctccatc
     1021 aggcggtcca gctttgccga tccggctttc gacttcgcta cggtcttcaa cttcagcagc
     1081 cggggatcgc agcaggcctg ccgcatcttc agcagcgcgt ccagaatgat gatccccgac
     1141 ctggccaggc ccttctgggc gatcgccgcc tgcaccttgg cgtgcatcga tagccggatc
     1201 gcctcgtaga tcgcgcgctg ggtggtttcc atctccaccg gctcgacgat ttccgtcttt
     1261 ggcggaagct cgctgatcac ctcctgcttg gtccgccgca gcatgaaggg gcggatgcgg
     1321 cgtgtcagca gttcctgccg ctcgacatcg ccatgttttt cgatcggcgt ccggtagcgc
     1381 gacttgaacg atttctgtcc gccgaggaac cccggcgcca ggaagtcgaa cagcgaccat
     1441 agttcgccaa ggtggttttg cagcggcgtg cccgacaggc acagccgttg ccgagcctgg
     1501 aggcgtaatg cctggcgggt ggtttcggcg ttggggtttt tgatcgattg cgcctcgtcc
     1561 aggatgaccg cgtgccattt ttgcttctgc aggacttcat ggtcgcggct cagcagggga
     1621 taggtggaga gcacgaggtc atgcttcgga atggccggga aatttgcctt gcgagccgga
     1681 ccatgcagcg gcaggacgga caattccggg gcgaagcggc gtgcctccgc cgtccagttg
     1741 gggatcaggc tggtcgggca tacgatcaat gacgggcggt ccaatcggcc ggctgccttg
     1801 tcgatcatca gatgggcaag cgtctgcacg gttttgccca gacccatgtc atcggccag
//
LOCUS       DASWQN010001336         3828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1715431, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001336 DASWQN010000000
VERSION     DASWQN010001336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715431"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3142
                     /locus_tag="VGC82_22670"
     CDS             <1..3142
                     /locus_tag="VGC82_22670"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEY0426137.1"
                     /translation="SPASAHQERTAINIYYLHPLVAGPLDTWAGTFSRIRAMGFSHVC
                     LAPPFEPGGSGDIFIHGSFDRLHPALGFSGPAEQGLGMAVELAAHAGLRLMLDVAPGQ
                     IAIDSPLRQRQPDWFDPVDHIQLPDPRRPPRRIDVATPRFNETTVADAVSDWWIGLLT
                     RLTGAGIEGFRCLTLDQVPASFWRRLTRTFPQALFLAWTPGMSGVRNLVGLGFDLTCS
                     SAGWWDGRSPCFLDEHNALQEVAPALGALEPSFVERLAQRLAPEADIAASYRLALRIA
                     GSTGAGLFVPMGFEYVTTRRFDSVLASPADLDAAKRDMRADLSEDIAAAIRLTTELAP
                     VETLRSLTSPASPVTALLRAEAVVLINPDIVRPSPAEFELAPLTAATGMAWSTESDVE
                     TPLRPGEVRILPCRPTHDIGGGSLELTRHWAETTRIAVEAVRPGGEFPAKTVVGREFV
                     ITADIFGDGHDVLAADVLWRAADASDWQRIPMLKLLNDKWEVRIRPDRIGLYWFAVEG
                     WWDQWGTFTHDLHAKVAAGQDVRLEIQEGQQLIKAALGRTEPAPTAEALLADSLGCAM
                     KQADSRPFASRSAVHSVRVDRQAAEFASWYELFPRSVTHDPATHGTFRSVMDRLPDIQ
                     AMGFDVLYFPPIHPIGRINRKGRNNSLRAEPDDVGSPYAIGSAEGGHDATHPALGTLD
                     DFRQLLRRTQQHGLEIAIDFAIQCAPDHPWVSQHKDWFRWRPDGSMRYAENPPKKYED
                     IVNPDFYGEASFPSVWIALRDVIQFWVDQGVRIFRVDNPHTKPLPFWHWMIADIQSRH
                     PDVLFLAEAFTRPKLMYRLAKVGFTQSYTYFTWRNTKQEITEYLTELTAPPPADFFRP
                     NFFVNTPDINPIFLQTSGRPGFLIRAVLAATLSGLWGMYSGFEICEAAPLPGREEYID
                     SEKYEIRPRDYAAPGNIVAEITALNRIRRQHPALQSHLGLVFYTAHNEQIIFYGKRDP
                     LDRSMVLVAVSLDPHIAQEAAIEVPLWEFGLDDQAAISVTDLMGGNHFMWYGENQRIR
                     LDPMDLPFRIWRLEAR"
     gene            3144..>3828
                     /locus_tag="VGC82_22675"
     CDS             3144..>3828
                     /locus_tag="VGC82_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0426138.1"
                     /translation="MNVGHPRPRIAQATDADPLWYKDAIIYQIHIKSFFDSNNDGIGD
                     FPGLISRLDYIADLGVNTIWLLPFYPSPRLDDGYDIADYRGVHSDYGTLADVKRFIAA
                     AHERGIRVIAELVVNHTSDQHPWFQRARLAKPGSMYRDFYVWSADDHKYAGTRIIFVD
                     SERSNWTWDETAKSYYWHRFFYHQPDLNFDNPRVMREVLSVLRYWAELGIDGLRLDAV
                     PYLIEREGTN"
BASE COUNT          695 a         1163 c         1194 g          776 t
ORIGIN      
        1 ctcccccgcg tcagcgcacc aggagcggac tgccatcaat atttattacc tgcatccgct
       61 cgtggccggc ccgctggaca cttgggcggg gacattttcg cgcatccgcg ccatgggctt
      121 cagccatgtc tgcctggcac cgccgttcga gcctggcgga agcggcgaca ttttcatcca
      181 tggatcgttc gaccggctgc atccggccct gggcttcagc ggaccagccg aacagggtct
      241 gggaatggct gtcgagttgg ccgctcatgc gggcttgcgg ctgatgctcg atgtggcgcc
      301 gggacaaatc gcgatcgact cgccgctgcg gcagcggcag cccgactggt tcgatccggt
      361 cgatcacatc cagttgccgg acccgaggcg gccgccgcgc cggatcgacg tcgcaacgcc
      421 ccgtttcaat gagaccacgg tcgcggatgc cgtgtcggat tggtggatcg gactgttgac
      481 ccggctgacc ggcgcgggga tcgagggctt ccgctgtttg acgctggacc aggttccggc
      541 ctcgttctgg cgccggctga ccaggacgtt tccacaagcg ttgttcctgg cctggacacc
      601 cggaatgagc ggcgtcagaa atcttgtcgg gttgggcttc gacctgacct gctcctccgc
      661 cggatggtgg gatggccggt caccctgctt cctggatgaa cacaacgcct tacaggaagt
      721 tgcccccgcg ctcggggcgc ttgaaccatc gtttgtcgag cgtttggcgc aacgcctggc
      781 gccggaggcc gatatcgccg cgtcctaccg tttggcactg cggatcgcgg gttcgaccgg
      841 agccggcctg ttcgtgccga tgggcttcga atacgtcacc acccggcgct tcgactcggt
      901 gcttgcaagc cccgccgacc tggacgccgc caagcgtgac atgcgggccg acctgtcgga
      961 ggacatagcg gcggcgatcc gactgacgac ggagttggcg ccggtggaaa cgttgcggtc
     1021 cctcaccagc cccgcatcgc cggtgaccgc gctgcttcga gccgaagcgg tggtactgat
     1081 caatccggat atcgttcgcc cgtcgcccgc ggaatttgaa cttgcgccgc tgacggcggc
     1141 cacgggaatg gcgtggtcca cggaaagcga tgttgagacg ccgcttcggc cgggtgaggt
     1201 acgcatcctg ccatgccgtc caacccacga tatcggcggc ggcagtctgg aactgacccg
     1261 gcattgggct gaaacgacac ggatcgccgt ggaagcggtg cggccgggcg gcgagtttcc
     1321 cgccaaaacc gtcgtcggcc gagagttcgt catcaccgcc gacattttcg gcgacgggca
     1381 cgatgttctg gcggccgatg tgttgtggcg ggcggcggat gcgtccgatt ggcagcgcat
     1441 cccgatgctg aagctgctca acgacaaatg ggaggttcgg atcaggccgg accggatcgg
     1501 tctttactgg ttcgccgtgg aaggatggtg ggaccagtgg ggcacgttca cccacgacct
     1561 gcacgccaag gttgccgcgg gccaggacgt aagactggaa atccaggaag gccagcagtt
     1621 gatcaaggcg gccctggggc gcaccgaacc ggcaccaacc gctgaagcct tgctcgcgga
     1681 cagccttgga tgcgcgatga aacaggcgga ttccaggccg tttgcctcac gcagtgcggt
     1741 tcattcggtc cgggtggacc ggcaagcggc ggagttcgcc agttggtatg aattgtttcc
     1801 gcgttcggtc acccacgatc cggcgacgca cggcacattt cggtccgtga tggaccgtct
     1861 cccggacatc caggcgatgg gtttcgacgt gctgtatttt ccgccgatcc atccgatcgg
     1921 ccggatcaac cgcaaggggc gcaacaacag tctgcgtgcc gaacccgacg acgtcggcag
     1981 tccgtatgcg atcggcagcg ccgaaggcgg tcacgatgcg acgcatcccg ctctcggcac
     2041 gctggacgat ttccggcaac tgcttcgccg aacccagcag cacggtctgg agatcgcgat
     2101 cgatttcgcc atccaatgcg cgccggacca cccctgggtc agccagcaca aggattggtt
     2161 tcggtggcgt cccgacggct cgatgcgcta cgccgagaat ccacccaaga agtacgagga
     2221 catcgtcaat cccgattttt acggtgaggc atcgttcccc tcggtgtgga ttgcattgcg
     2281 cgacgtgatc cagttctggg ttgaccaggg ggtgcgtatc ttcagggttg acaaccccca
     2341 taccaagcca ttgccattct ggcattggat gatcgccgac attcaaagcc ggcatccgga
     2401 cgtcctgttc ctggcggaag cgttcacccg gccgaaactg atgtaccgac tggcgaaggt
     2461 cggcttcacg cagtcctata cgtactttac ttggcggaac acgaagcagg agattacgga
     2521 atatctcacc gaactgacgg ccccgccgcc ggcggatttt tttcggccta atttcttcgt
     2581 caacacgccc gacatcaatc cgattttcct gcagacatcc gggcggcccg gttttctgat
     2641 cagggcggtg ctggccgcca cattgtccgg attgtggggg atgtattcgg gcttcgaaat
     2701 ctgtgaggct gcgccgctac cgggccgcga agaatatatt gattccgaga aatacgagat
     2761 ccggcctcgg gattatgcgg caccaggcaa catcgtggcg gaaatcaccg cgctcaaccg
     2821 gatcaggcgt cagcaccccg ccttgcagtc gcacctcggt ttggtatttt acaccgctca
     2881 caatgagcag atcatttttt atggaaaacg tgaccctctc gatcgcagca tggtcctggt
     2941 ggctgtcagc cttgatcccc acatcgcgca ggaggctgca atcgaggtgc cgctgtggga
     3001 atttgggctg gacgatcagg cggcgatctc ggtgacggat ctgatgggcg ggaatcattt
     3061 catgtggtac ggcgaaaatc agcgcatccg gctggatccc atggatttgc cattccgcat
     3121 ttggcggctc gaggcgcgat gatatgaatg ttggccaccc gcggccgcgc atcgcgcaag
     3181 cgaccgacgc agatccactt tggtataaag atgcgatcat ctatcaaatc cacatcaaat
     3241 ctttcttcga tagcaataat gatggcattg gcgattttcc aggtttgatt tcgcggctgg
     3301 actatattgc cgaccttgga gtcaatacga tttggctgct gccgttctac ccttcgccac
     3361 gattggacga cgggtatgac atcgccgact accgcggggt gcactcggat tacggcacgc
     3421 tggcggatgt gaaacggttc attgccgcgg cccatgaacg cggtattcgc gtcatcgcgg
     3481 agttggtggt caatcacacc tccgaccagc atccgtggtt ccagcgtgcg cggcttgcca
     3541 aacccggctc gatgtaccgg gacttctacg tctggtccgc cgacgaccac aaatacgccg
     3601 gcacccgcat cattttcgtc gactcggaac ggtcgaactg gacgtgggac gaaaccgcga
     3661 agtcgtacta ttggcaccga tttttttatc atcagcccga cctgaacttc gacaacccgc
     3721 gggtgatgcg cgaggttctg tcggtgcttc gctattgggc cgaacttggg atcgacgggc
     3781 tgcgcctgga cgcggtgcct tacttgatcg agcgcgaggg taccaaca
//
LOCUS       DASWQN010001337         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_527422, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001337 DASWQN010000000
VERSION     DASWQN010001337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527422"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VGC82_22680"
     CDS             complement(<1..486)
                     /locus_tag="VGC82_22680"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY0426139.1"
                     /translation="MGVTSAILGTPMLARDQRTPFSEWWWTVDKLLLAAISALMLAGV
                     ILSLAASPPVATRIGLDPFHFFSRHVMFLLPSFMVLIAVSFMSPKAIRRSALLVFAVS
                     VLLIVATLAIGPEVKGSRRWITLLGVNIQASESAKPAFVVIAAWLFAESTKRPEMPAT
                     SM"
     gene            639..>1538
                     /locus_tag="VGC82_22685"
     CDS             639..>1538
                     /locus_tag="VGC82_22685"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY0426140.1"
                     /translation="MRSFARFVTASLLALVSQAAAARDGEKWVVSWTGPVQGPYPVGN
                     PSAQPDQKFAFPEPAAGARDQTFRLMVRPDIWGRQARIRLSNAFGKKPVTFDGVYAGL
                     QLGSAALVRGTNRPVTFGGKSSITVSPGDSVWSDPVALAFVKQPDDLAGRRLAVSFHV
                     AGDSGPMTWHAKALTTSYVTAPGAGAKGALEDEAAFPYTTASWFFLDAVEMMAPSDAF
                     AIMAFGDSITDGTASTMNGDDRWPDVLSRRLKALYGNKVAVVNGGIGGNQIAGPAEYG
                     ANKPFPGGPASGQRLDRDVLTLSG"
BASE COUNT          275 a          495 c          523 g          245 t
ORIGIN      
        1 catcgaggtc gccggcattt ccggccgctt ggtcgattcc gcgaacagcc acgccgcgat
       61 caccacgaac gcgggcttgg cggattcgga agcctggatg ttgacgccga gcagcgtgat
      121 ccagcgccgc gaacccttga cctccggccc gatcgccagc gtcgccacga tcaggagaac
      181 ggacaccgcg aacaccagca gcgccgagcg gcggatcgcc ttcggcgaca tgaacgacac
      241 cgcgatcagc accatgaagg acggcagcag gaacatcacg tggcggctga agaaatgaaa
      301 gggatcgagc ccgatccgcg tcgccaccgg cgggctagcc gccagcgaca ggatgacgcc
      361 ggccagcatc agcgccgata tcgcggcaag cagcagcttg tccacggtcc accaccattc
      421 cgagaacggg gtgcgttggt cacgggcgag catgggcgtc cccagaatgg cagaggtaac
      481 gcccattggt ggccgaggat ggttgccaag gggttaatgg gatcggtaac ttttgaggga
      541 tgcgcggtgg cccgctggcg ggcgacgacg gcttggcatc ggctggccgg aaactctagg
      601 atgtcgccaa cgataacaaa atctggagga aacccgtcat gcgttcattc gcccgcttcg
      661 tcacggcttc gctgctcgct ctggtatcgc aggccgccgc cgcccgggat ggcgaaaagt
      721 gggtggtgtc gtggaccggc ccggtgcagg ggccctaccc cgtcggcaat ccatccgccc
      781 agcccgacca gaaattcgcc tttccggagc cggcggccgg cgcgcgggat cagacgtttc
      841 ggctgatggt gcggccggac atctggggac gccaggcgcg catccggctc agcaacgcct
      901 tcggcaagaa gccggtgaca tttgatggtg tgtatgccgg cctccagttg ggaagtgccg
      961 cgctggtgcg cgggaccaat cgcccggtga cgttcggcgg aaaatcttcg atcacggtgt
     1021 cgccgggaga ctccgtatgg agcgatccgg ttgcgctggc ttttgtaaaa cagccggatg
     1081 atcttgcggg ccgccggctg gcggtgagtt tccacgtcgc cggtgacagc ggcccgatga
     1141 cctggcacgc caaggcgctg accacctcct acgtcaccgc gcccggcgcc ggcgccaagg
     1201 gcgcgctgga ggacgaagcg gccttcccct acaccacggc gtcgtggttt ttcctcgacg
     1261 cggtcgagat gatggcgcca tcggatgcgt tcgcgatcat ggcgttcggc gattccatca
     1321 ccgacggcac cgcctcgacc atgaacggcg acgaccgctg gcccgacgtg ctgtcgcgtc
     1381 gcctcaaggc gctctacggc aacaaggtcg cggtagtgaa tggcggcatc ggcggcaacc
     1441 agatcgcggg acctgccgag tatggtgcga ataagccgtt tcccggcggc ccagcctcgg
     1501 gccagcggct cgatcgcgac gtgctgacgc tctcgggc
//
LOCUS       DASWQN010001338         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1187123, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001338 DASWQN010000000
VERSION     DASWQN010001338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187123"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1295)
                     /gene="mtnA"
                     /locus_tag="VGC82_22690"
     CDS             complement(207..1295)
                     /gene="mtnA"
                     /locus_tag="VGC82_22690"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEY0426141.1"
                     /translation="MKIAGTEYRSVWRDADDGWSVHILDQTRLPWALEILRLTTCDDA
                     ARAIRSMQARGAPLIGAVAAYGLCLALRGDTSTEAMERDAALLNATRPTAVNLRWALD
                     RMLIRLRNTLPAERVAVAYAEAALIADEDVAQNEAIGQHGLKLIEAAVRPGRPVNVLT
                     HCNAGWLATVDWGTALAPIYTAFNAGIDLHVWVDETRPRNQGAALTAWELGKHGVPHT
                     VVADNAGGHLMQHGMVDLVIVGADRVSRTGDAANKIGTYLKALAAHDNAVPFWVAMPS
                     TTIDWTLSDGVADIPIEERSAAEVTDMTGRLPDGTIATMRIVSAGSQAANPAFDVTPA
                     RLVTGLITERGCCAASSDGLLGLFPERR"
     gene            complement(1292..1792)
                     /locus_tag="VGC82_22695"
     CDS             complement(1292..1792)
                     /locus_tag="VGC82_22695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HEY0426142.1"
                     /translation="MTGIAVTTSRLDRVILDGDSPNNLSPFIEADREQAVADLAAANH
                     FAPLIGTENPGPYALHLSIQEGRLIFDIRNTDGGRLTAIGLALGPFRRLVKDYQLLVD
                     SHIKAVEEGREQRIQAIDMGRRGLHNEGAELMTQRLAGKIDIDFDTARRLFTLVCVLH
                     QRVSSR"
     gene            2008..2661
                     /locus_tag="VGC82_22700"
     CDS             2008..2661
                     /locus_tag="VGC82_22700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY0426143.1"
                     /translation="MARILVINPNCSDACSVGISQALQPFRLAGGPAIDVVTLKEGPP
                     AIYTWRDWHSVVEPMCRLVERSDADVHVIACASDPGVEAVRTATPRPVLGIFRSAVAA
                     AAARAERFGVIAVVESSKARHRAALRAMGLESRLAGEVALNLSMDVLLDAAAAQAALV
                     SAGRELAAMGAETIILGCTGMAHHRAAVREAVGIPVIEPVAAAAGLALGLVLGEGPQ"
BASE COUNT          465 a          903 c          952 g          484 t
ORIGIN      
        1 accgaaggac cgccgacatg gcaacgatcc gcacgctcac cttggccctt gttgtcctga
       61 tcggcatcgc gccgatgctg ggcgcctatc ataccgctgc cggggttggg gaggatctct
      121 ccgcgggcgg ccaggcgatc agcaacagcg ccaagaagct tacgccctga tggcggcggc
      181 tgtggtccgg aggtcgcaaa cagggtctaa cgccgctccg ggaacagccc cagcagtccg
      241 tccgatgagg ccgcacagca gccgcgttcg gtaatcagac cggtgaccag gcgcgcaggg
      301 gtgacgtcga aagccggatt ggcggcttgg ctgccggcgg aaacgatgcg cattgtcgcg
      361 atggtcccgt ccggcagtcg gcccgtcatg tcggtcacct cggcggcgga ccgttcctcg
      421 atgggaatgt cggcgacacc atcgctcagc gtccagtcga tcgtggtgga cggcatcgcg
      481 acccagaacg ggactgcatt gtcatgcgcc gccagggcct tcaggtaggt gccgatcttg
      541 ttggcggcgt cgcccgtgcg ggaaacccgg tcggcgccga cgatcaccag atcgaccatg
      601 ccgtgctgca tcaagtggcc gccggcgttg tccgccacca ccgtgtgggg cacgccgtgc
      661 ttgccgagtt cccaggccgt cagcgcggca ccctggttgc gcgggcgcgt ctcgtccacc
      721 cagacatgca gatcgatccc ggcgttgaac gcggtgtaga tcggcgccag cgcggttccc
      781 caatcgacgg tggccagcca cccagcgttg caatgcgtca gcacattcac cggacgaccc
      841 ggccgcacgg ccgcctcgat cagcttcagc ccgtgctggc cgatcgcctc gttctgcgcg
      901 acatcctcgt ccgcgatcag cgccgcttcg gcataggcga cggcaacgcg ttcggcgggc
      961 aaagtgttcc gcagccggat cagcatccgg tccagcgccc agcgcaggtt gacggcggtc
     1021 ggacgggtcg cgttcagcag cgccgcgtcg cgctccatcg cctcggtgga cgtatcgccc
     1081 cgaagggcca ggcaaagccc ataggccgcc acagcaccga tcagaggcgc gccacgggcc
     1141 tgcatcgacc tgatcgcccg ggcggcgtca tcgcaggtgg tcaggcgcag gatctccagc
     1201 gcccagggca accgggtctg gtccaggatg tggaccgacc agccatcgtc ggcgtcgcgc
     1261 cagacgctgc gatactcggt gccagctatt ttcatctcga ggagactctt tggtgcagca
     1321 cgcaaaccag cgtgaacagc cggcgcgcgg tgtcgaaatc gatgtcgatc ttgccggcca
     1381 gccgctgggt catcagctcc gcaccttcgt tgtgcaagcc gcgacggccc atgtcgatgg
     1441 cctggatgcg ctgctcgcgg ccttcctcaa ccgctttgat gtggctatcg accagcaact
     1501 ggtagtcctt caccaaccgg cggaacgggc ccagcgcaag gccgatcgcg gtcagccggc
     1561 caccatcggt gttgcggata tcgaagatca ggcggccttc ctggatcgac aggtgcagcg
     1621 cgtaggggcc cgggttctcg gtgccgatca acggcgcgaa atggttcgct gccgccaggt
     1681 cggcaacagc ctgttcgcga tccgcctcga tgaaagggga cagattgttg ggagaatccc
     1741 catccaggat cacgcgatcc agccgagagg tcgtgacggc gataccggtc atatctggtt
     1801 acagcctgat aaatggggcc aggaattcgt tgcggcagac cacgcgggct gccacggagg
     1861 cgggttcatg ttcaggtcct tctggccgct cgcgggacca tgcccacaac gcacgaacga
     1921 cgatcggcac gcaaggtaac ccttgctgcg ctgcaatacc atatcggttc tcttgattcc
     1981 ggatctcagc ggcggaagag tggccgcatg gcacgtattc tggtgatcaa tccaaactgc
     2041 tccgatgcct gttcggtcgg catttcgcaa gcgctgcaac catttcggct tgctggagga
     2101 ccggccatcg atgtggtgac actgaaagaa ggcccgccgg cgatctatac ctggcgggac
     2161 tggcactcgg tggtggaacc catgtgccgg ctggtggagc gatccgatgc cgatgttcat
     2221 gtcatcgcct gtgcctccga tccgggggtc gaggcggtgc gaaccgccac accgcggccg
     2281 gtgttgggga tctttcgttc ggccgtcgcc gccgcggcgg ctcgggcgga acggttcggg
     2341 gtcatcgcgg tcgtcgagtc ctccaaggct cggcatcgcg ccgcgctgcg cgccatgggc
     2401 ctggagtcgc ggctggcagg tgaggtggcg ctgaaccttt cgatggacgt gctgctggat
     2461 gccgcggccg cacaggcagc tttggtctcg gcggggcggg aactggcggc gatgggggcg
     2521 gaaaccatca tcctgggatg caccgggatg gcgcaccatc gcgcggcggt gcgggaggcg
     2581 gtgggaatac cggtgatcga accggtcgcg gctgccgcag ggctggcgtt ggggctggtg
     2641 ctgggggagg ggccgcagtg aacgcgaatg tttagccaga ccgtgacttc cgccacaccg
     2701 gaggaaccac cgaacctacc tgttcggtaa tccggccata cgcttccggc cgccgatgct
     2761 tgtcgaacgc gaaaatcgtg gtgcggccca gcgtgcacat ctcc
//
LOCUS       DASWQN010001339         5097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2506840, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001339 DASWQN010000000
VERSION     DASWQN010001339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2506840"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..349
                     /locus_tag="VGC82_22705"
     CDS             20..349
                     /locus_tag="VGC82_22705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0426144.1"
                     /translation="MSFGADPQNVEKARQLVVHDLKAMQTNQVDESELTRAKAELLRR
                     LPMQRASIHGIAGQYLRLTELGLPLDSAQTAAERYLAITATEIQQAFAKWLRPDDLVQ
                     VVKGPQE"
     gene            complement(415..1980)
                     /locus_tag="VGC82_22710"
     CDS             complement(415..1980)
                     /locus_tag="VGC82_22710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEY0426145.1"
                     /translation="MSTTLIRAERPLFEGADWEFGTIQRCYDEIEKVALNEMGLSIYT
                     NQIEVIGTEQMLDAYSSIGMPLFYKHWSFGKHFSRNEAMYRHGMQGLAYEIVINSNPC
                     ISYIMEENTAMMQTLVMAHAAFGHNHFFKNNYLFQQWTDANGILDYLEFAKDYIMSCE
                     ERYGEAAVERLLDAAHALMNHAVHKYPRKRAADLRSEEKRERERREHDEKVFNDLWRT
                     VPGKENTRADPTAAEQRKALLELPQENILYFMEKTAPRLAPWQREVLRIVRIIAQYFY
                     PQTQTKVMNEGCATYSHYTIMNRLHEKGLLTDGAMVEFLSSHTNVVFQPEFDDRRYSG
                     LNPYALGFAMMQDIERICTLPTAEDHQWFPHIAGCNDHMNVLRDVWANYRDESFILQY
                     LSPKLVRQFGLFHVMDDASEPNLRVEAIHDERGYRKIKQALAKQYDVSWNSADIQVVD
                     VNLAGDRRLIIHHRALNRVLLEEKDARLVLQHLADLWGYDVLMKEIDPSTDAVLKEHA
                     ASPKPGIVRIETT"
     gene            complement(1977..3278)
                     /locus_tag="VGC82_22715"
     CDS             complement(1977..3278)
                     /locus_tag="VGC82_22715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HEY0426146.1"
                     /translation="MHIVDRRLNPSGKNLANRQRFMRRAKAQIRKAVHESAGSRSIKD
                     ADEGGEVVLPAQGVHEPSFRRSGSGGIRDHLLPGNKEYIAGDTIQRPPQGGGGSGSEG
                     SPDGDGEDDFRFALSREEFVDLFLEDLELPDLAKRRVVNADALVYHRAGYSVSGSPSN
                     LALNRTVRNSLSRRIALRRPKPEELQALRDEIEALEPADEDKRVALQEELARKQRRTG
                     MIPYIDPLDVRYRRFEAHPKPVAQAVMFCLMDVSGSMTEHMKDLAKRFYVLLYIFLKR
                     RYKNVEVVFIRHTHQASEVDEETFFNSPETGGTVVSTALDEMKRVIDERYSPDNWNIY
                     AAQASDGDNTASDNDKTAALLTKVLLPACQYYAYLEVGRDSEHFPPGFIRRDSDLWQT
                     YSTLIKAGAPMAMRKVGHRRDIYPVFRELFAKKTGVEAAAE"
     gene            complement(3318..>5097)
                     /locus_tag="VGC82_22720"
     CDS             complement(3318..>5097)
                     /locus_tag="VGC82_22720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEY0426147.1"
                     /translation="DTSKDPRLGRIFMNRTIRVYPAFNEFYGMEETIERIVGFLRHAA
                     QGLEERKQIMYLLGPVGGGKSSLAERLKALMETFPIYVLKAGDDLSPVFESPLGLFDP
                     ERMGSLLEDRYNIPRRRLTGLMSPWCIKRLDEFHGDISKFRVQKIIPSRLRQIGIAKT
                     EPGDENNQDISSLVGKVDIRKLEMHAQNDPDAYSYSGGLNRANQGMLEFVEMFKAPIK
                     MLHPLLTATQESNYIGTENIGAIPFQGIIMAHSNESEWQSFKNNKNNEAFIDRIYVIK
                     VPYCLRVTEEQKIYEKLVTRSELGNAPCAPATLEMLARFSVLSRLKRHENSTIFAKMR
                     VYDGESLKETDPRARSLQEYKDTAGVEEGMDGASTRFAFKVLAATFNHDTTEIAADPV
                     HLMYVLEQSIRREQMPQETEKRYLEFIKGELAPRYAEFIGNEIQKAYLESYHDYGQNL
                     FDRYVSYADAWIEDIDHKDADTGQLLNRELLNQELTKVEKPAGIANPKDFRNEVVKFC
                     LRARANNNGKNPSWTSYEKIRDVIERRMFSQVEELLPVISFGSKKDSDTEKKHSDFVD
                     RMTARGYTERQVRRLVEWYMRVKQAG"
BASE COUNT          923 a         1548 c         1487 g         1139 t
ORIGIN      
        1 ctcccgatcc gattacagtg tgtcgtttgg cgccgatccg cagaatgtgg agaaagcgcg
       61 ccagctggtc gtgcatgacc tgaaggccat gcaaaccaac caagttgacg agagcgaact
      121 gacgcgggcg aaggcggaac tgctgcggcg tctgcccatg cagcgcgcca gtatccacgg
      181 catcgccgga caatatttgc gcctgaccga actgggcctg ccgctggatt cggcgcaaac
      241 cgcagcagag cgatatcttg ctatcaccgc gacggagata cagcaggcgt ttgccaagtg
      301 gctgcgtccg gacgatctgg tgcaggtcgt gaaagggccg caagagtagg ggcgggcctt
      361 ctcccaatgg ccgtggcccg tgccgggccg aacggccgga ggacccgcct tttcttaggt
      421 tgtctcgatc cgcacgatgc cgggcttggg cgacgcggca tgttccttca gcacggcatc
      481 ggttgaagga tcgatttcct tcatcagcac atcgtagccc cacaggtcgg ccagatgctg
      541 cagcacgaga cgcgcatcct tttcctccag cagcacccgg ttgagcgcac ggtggtggat
      601 gatcaatcgc cggtctccgg caagatttac gtccacaacc tgaatgtcgg cggagttcca
      661 cgacacgtcg tactgctttg ccagtgcctg cttgatcttg cgatagccac gctcatcgtg
      721 gatcgcctcg acccgcaggt tcggctcact cgcgtcgtcc atgacgtgga acaggccgaa
      781 ctggcggacc agtttcgggc tgaggtactg caggataaaa ctctcgtccc ggtagttagc
      841 ccacacgtcg cgcagcacgt tcatatggtc gttgcagccg gcaatatgcg ggaaccactg
      901 atggtcctcg gcggtcggca gcgtgcagat ccgctcgatg tcctgcatca tggcaaatcc
      961 cagcgcatag ggattgaggc cggagtaacg gcggtcgtcg aattcaggct ggaagacgac
     1021 gttggtatgg gagctcagaa actccaccat ggcgccgtcc gtcagtaggc ccttttcgtg
     1081 cagccggttc atgattgtgt aatgggagta ggtggcgcag ccctcgttca tcaccttggt
     1141 ctgcgtctgc ggatagaagt actgggcgat gatccggacg atgcgcagaa cctcgcgctg
     1201 ccagggtgcc agccttggcg cggtcttctc cataaaatac aggatgtttt cctgcggtag
     1261 ttccagcagc gccttgcgtt gctccgccgc cgtcgggtcg gcgcgggtat tttccttgcc
     1321 tggcacggtg cgccataggt cgttgaacac tttctcatcg tgttcgcgcc gttcgcgctc
     1381 gcgcttttcc tcggaccgca gatcggcggc gcgcttgcgc ggatatttat gtacggcatg
     1441 attcatcagc gcatgagccg cgtccagcag ccgctcgacc gcggcctcac cgtagcgttc
     1501 ttcgcacgac atgatgtagt ctttggcgaa ttccaggtaa tccaggatcc cgttggcgtc
     1561 ggtccactgc tggaacagat agttgttctt gaagaagtgg ttatgtccga acgccgcatg
     1621 cgccatcacc agcgtctgca tcatggcggt gttctcttcc atgatgtagc tgatgcacgg
     1681 gttggaattg atgacgatct cgtacgccag cccctgcatg ccgtgccgat acatcgcctc
     1741 gttgcgggag aaatgcttgc cgaacgacca gtgcttataa aacagtggca tcccgatcga
     1801 ggaataggca tccagcatct gctcggtgcc gatgacctcg atctggttgg tatagatact
     1861 caggcccatt tcgttcaggg cgactttctc gatctcgtcg tagcaccgtt ggatggtgcc
     1921 gaactcccag tcggcgcctt cgaacagcgg gcgttcggcc cggatcaatg tcgtgctcat
     1981 tcggctgcgg cctccacccc ggttttcttt gcgaacaact cacggaacac cggatagata
     2041 tcccgccggt gcccgacctt ccgcatcgcc attggcgcgc ccgctttgat aagcgtggaa
     2101 taggtttgcc aaaggtcgct gtcgcggcgg atgaagccgg gcggaaaatg ctccgagtcg
     2161 cgaccgactt ccagatacgc gtagtactgg cacgccggca gcagtacctt ggtcaacagc
     2221 gccgccgtct tgtcgttgtc gctggcggtg ttatcgccgt ccgatgcctg ggctgcgtaa
     2281 atgttccagt tatccgggct ataccgctcg tcgatgaccc gcttcatctc gtcgagcgcg
     2341 gtggacacca cggtgccacc ggtctccgga ctgttgaaga aggtctcctc gtcgacctcg
     2401 gaggcctggt gcgtgtggcg gatgaagacc acctcgacgt tcttgtagcg gcgcttcaag
     2461 aagatataca gcagcacgta gaatcgcttc gcgaggtcct tcatgtgttc ggtcatcgat
     2521 ccggacacgt ccatcaggca gaacatcacc gcctgggcaa ccggtttcgg atgcgcctcg
     2581 aaccgcctgt agcgcacatc gagcggatcg atgtagggga tcatcccggt gcggcgttgc
     2641 ttgcgggcca actcttcctg cagcgcaacg cgcttgtcct cgtcggcggg ctccagggct
     2701 tcgatctcat cccgcaacgc ctgcaattct tccggctttg gccggcgcag cgcgatccgg
     2761 cgcgacaggc tgttccgcac cgtacggttc agcgccagat tcgacggcga gccactcacc
     2821 gagtaaccgg cacggtggta aaccagcgca tcggcgttca ccacccggcg tttggccagg
     2881 tcgggcagtt ccagatcctc caggaacagg tcgacgaatt cctcgcgcga cagggcgaaa
     2941 cggaagtcgt cctcgccgtc gccatccggg ctgccctcag acccgctgcc gccgccgccc
     3001 tgaggcggcc tttggatcgt gtcgccggcg atgtactcct tgttccccgg caacagatgg
     3061 tcgcggatgc cgcccgaacc gctgcgccgg aacgaaggtt cgtgcacccc ctgagccggc
     3121 agaacgacct cgccgccttc atcggcatcc tttatgcttc ggctgccggc actttcgtgc
     3181 accgccttgc ggatttgcgc tttcgcgcgc cgcatgaagc gctggcgatt agccaggttc
     3241 ttgccactgg ggttcaggcg ccggtcaact atgtgcatag tttgtctatt ggacctctcc
     3301 ggtgatcggc gggacgccta tcccgcctgt ttcacacgca tataccactc aaccagccgt
     3361 ctgacttgcc tttcggtata accgcgggcg gtcatgcgat cgacgaagtc gctgtgtttc
     3421 ttttcggtat cactgtcctt cttcgaaccg aagctgataa ccggcaacag ctcttcaacc
     3481 tggctgaaca tccggcgttc gatcacgtcg cgaattttct cgtagctggt ccacgacggg
     3541 ttcttgccgt tgttgttcgc tcgggcgcgg aggcaaaact tcacgacctc gttgcggaaa
     3601 tccttgggat tggcgatgcc cgccggcttc tcgactttgg tcaactcctg gttgagcaac
     3661 tcccggttca gcaactgccc tgtatcggcg tccttgtggt ctatgtcctc gatccaggca
     3721 tcggcatagg acacataccg gtcgaacagg ttctggccgt agtcgtgata cgactccaga
     3781 taggctttct ggatctcgtt gccgatgaat tctgcgtagc gcggtgccag ttcgcccttg
     3841 atgaactcca gataccgctt ttccgtctct tgcggcatct gctcgcggcg gatggactgt
     3901 tccagcacat acatcagatg caccggatcg gccgcgattt cagtcgtgtc atggttgaac
     3961 gtcgcggcaa gcaccttgaa ggcgaagcgg gtcgaagccc cgtccattcc ttcctcaacc
     4021 cccgcggtat ccttgtattc ttgcaaggac cgcgcgcgcg gatcggtctc tttcaggctt
     4081 tcgccgtcat aaacgcgcat tttcgcgaag atggtcgagt tctcgtgccg cttcaggcgc
     4141 gacaacaccg agaaccgcgc cagcatttcc agcgtggccg gcgcgcaggg agcgttgccg
     4201 agctccgaac gggtaaccag cttctcgtag attttctgct cttcggtcac ccgcaggcag
     4261 tacggaactt taatgacata aatgcggtcg atgaaggctt cgttgttctt gttgttcttg
     4321 aaggactgcc actccgactc gttggagtgc gccatgatga tcccctggaa tgggatcgcg
     4381 ccgatatttt cggtgccgat gtagttgctt tcctgcgtcg cggtcagcaa cggatgcagc
     4441 atcttgatcg gtgccttgaa catctcgacg aactcgagca tgccctggtt cgcccggttc
     4501 aacccccccg agtagctata ggcatcggga tcgttctggg cgtgcatttc cagcttgcgg
     4561 atatcgacct tacctaccag cgaggagatg tcctggttgt tctcgtcacc aggttcggtt
     4621 ttagcaatcc cgatttgccg caaccgcgac gggataatct tttgaacgcg gaacttggag
     4681 atgtcgccat ggaattcatc cagccgcttg atgcaccacg ggctcattaa acccgtaagc
     4741 cgccgccgcg gaatgttgta gcgatcttcc agcaaagacc ccatccgctc ggggtcgaac
     4801 aacccaagcg ggctttcgaa taccggactg agatcgtcgc ccgctttcag tacgtagatg
     4861 ggaaaagtct ccatcagcgc cttcaggcgt tccgcgaggg atgacttgcc gccgccgacc
     4921 ggcccgagca ggtacatgat ctgcttacgc tcttcgagac cctgagcagc atggcgcagg
     4981 aagccgacga tacgctcgat cgtctcttcc atgccgtaga attcgttgaa agcagggtac
     5041 acccgaatgg tccggttcat aaagatccgg ccgagacgcg gatctttgga ggtatcg
//
LOCUS       DASWQN010001340         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_527662, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001340 DASWQN010000000
VERSION     DASWQN010001340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527662"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VGC82_22725"
     CDS             <1..626
                     /locus_tag="VGC82_22725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0426148.1"
                     /translation="LSWIVGIPVAVISALRPNSLGDNISRFVSILFLAIPGFWLGMLI
                     MLASLFWFGYRAPLTGASLFTDPWTNLEIVVGPAVVLGLGQAAYIARMARSSLLEVVR
                     EDYVRTARAKGLNGRMVITWHALPNALLPVITLSGILLGFVLAGSIPVERAFGTPGLG
                     FAMFTAVNERDVFVMQNLVLLYAVVFVVLNILVDITIAWLDPRIRHQ"
     gene            623..1543
                     /locus_tag="VGC82_22730"
     CDS             623..1543
                     /locus_tag="VGC82_22730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0426149.1"
                     /translation="MTVIEADNEVLAPRRVGAARASWLRRAGRNLILFVRHSPLSAFW
                     GLIAAGIIVVSVAAPVFAPYEPLKVDFRRMTKPPSEMHYFGTDQIGRDTLSRVIYGGR
                     ASLTVAVSAVLFGTTLGALWGLASGYLGGRFDIISQRLIEFLQSFPDLILAMAIAMAL
                     GAGTGTVIVAIAITRVPFGGRVIRSVVLSLKEMAYVESARGLGASKMRIMLKHILPQC
                     MAPFLILATTHLGVAIIIEASLGFLGVGIPPPTPTWGNMLSDSLNAGLVPPWWLVLFP
                     GLAITLTVLAFNLLGDGIRDRLDPRLRGSV"
     gene            complement(1535..>1855)
                     /locus_tag="VGC82_22735"
     CDS             complement(1535..>1855)
                     /locus_tag="VGC82_22735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY0426150.1"
                     /translation="LGRIVEYADKDTLFRRPMHPYTEALLAAVPVAKSGARDRQRLIV
                     KGDVPSPINPPSGCHFHTRCIYATARCRHEAPALREIVPGHLAACHLHDAEVRFPLMG
                     SASD"
BASE COUNT          275 a          607 c          588 g          385 t
ORIGIN      
        1 tgctatcctg gatcgtcggc atcccggtcg ctgtcatcag cgcgcttcgg cccaacagcc
       61 tcggcgacaa catttcccgg ttcgtgagca tcctgtttct cgcgatcccc ggtttctggc
      121 tgggcatgct catcatgctc gcgtccttgt tctggtttgg ctatcgcgcg ccactgaccg
      181 gtgcgtcctt gttcaccgat ccatggacca atctggaaat cgtcgtgggc cccgcggtcg
      241 tcctggggct ggggcaagct gcctatatag cccgcatggc gcggtccagc ctgctggagg
      301 tcgtccgcga ggactatgtg cgaaccgccc gggccaaagg attgaacggg cgcatggtca
      361 tcacctggca cgccctgccg aacgccctgc tgcccgtcat caccctgtcc ggtatcctgc
      421 tcggttttgt gcttgccgga tcaatcccgg tggaacgtgc gttcgggacg ccgggacttg
      481 gtttcgccat gttcaccgcg gtgaacgagc gggatgtctt cgtcatgcag aacctggtgc
      541 tgctgtacgc cgtcgtgttc gtggtgctga acatccttgt cgatatcacc atcgcctggc
      601 tcgacccgag gatccgccat caatgaccgt catcgaagca gacaacgagg tcctggcacc
      661 acggcgcgtt ggggcggccc gggcatcgtg gctccggcgc gctggccgca acctgatcct
      721 gttcgtcaga cactcgccgc tgtcggcgtt ctggggcctt atcgccgccg gcatcatcgt
      781 tgtttcagtc gccgccccgg tcttcgcgcc ctatgaaccc ctgaaggtcg atttccggcg
      841 catgaccaag ccaccgagcg aaatgcacta ttttggcacc gaccagatcg gccgggatac
      901 gctcagccgg gttatctatg gcggccgagc atcgctgacc gtggccgtat ccgccgtgtt
      961 gttcggcacc acgctgggtg cgctgtgggg gctggcaagc ggctatctcg gcggccggtt
     1021 cgatatcatc agccagcgcc tgatcgagtt cctgcagtcg tttccggacc tgatcctggc
     1081 catggccata gccatggcgc tcggtgcagg caccggcacc gtgatcgtcg ccatcgccat
     1141 cacccgagtg ccgttcggcg gccgggttat ccgatccgtg gtgctgtcgc tgaaggaaat
     1201 ggcctacgtc gagtcggcac gcggtttggg cgcgtcgaaa atgcgcatca tgctgaagca
     1261 catcctgccg caatgcatgg cgcccttcct gatcctggca acgacccacc ttggcgtcgc
     1321 aatcatcatc gaggcctcgc tcggttttct tggcgtcggc attccgccgc caaccccaac
     1381 ctggggcaac atgctgtccg actcgctgaa cgccgggctg gtgccgccgt ggtggctggt
     1441 gctgttcccc ggcctcgcca tcaccctgac cgtgctggcg ttcaacctgc ttggtgacgg
     1501 tatccgcgac cggctggacc cgcggcttcg gggatcagtc tgaggcggaa cccatcagcg
     1561 ggaaacgcac ctcggcatca tgtaggtggc aggctgccag gtgtccgggg acgatttccc
     1621 gcagcgccgg cgcctcatgc ctgcagcgcg ccgtcgcgta aatgcagcgc gtgtggaagt
     1681 ggcatccgga agggggattg atcgggctgg ggacatcgcc tttgacgatc aggcgctggc
     1741 ggtcgcgcgc acccgacttc gccaccggca ccgcggccag cagcgcttcg gtatacgggt
     1801 gcatcgggcg gcggaagagc gtgtccttgt cggcatattc gacgatgcgg ccgag
//
LOCUS       DASWQN010001341         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_1187331, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001341 DASWQN010000000
VERSION     DASWQN010001341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187331"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1631
                     /gene="xdhA"
                     /locus_tag="VGC82_22740"
     CDS             225..1631
                     /gene="xdhA"
                     /locus_tag="VGC82_22740"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HEY0426151.1"
                     /translation="MRDTIHFRLGQENRALRDIDPTMTVLEYLREPERLYGTKEGCAE
                     GDCGACSVVLVDQQGRYQAVNACILFMTALDGKQLLTVEHLAAADGTLHPVQQAMVDH
                     HGSQCGFCTPGFVMSIFALRTEAKPDRAAVNEALAGNLCRCTGYRPIVDAALSVCTGH
                     HNPPAVALPVPQGTLALTNGDKYSFSPRCGDELARILLDHPQAVIVAGGTDVGLWVTK
                     QHRALPVTVSLDAVADLREVSETADTIRIGAAATYHDALPMLEKHFSDFGAMIRRIGS
                     RQIRNRGTIGGNIANASPIGDSAPVLLALDASLVLRLGNTRRTVKLDDFFAGYRRTVL
                     APSEFIERIDVPIPRPGELFRCYKVSKRFDQDISAVCGAFRLTLQDGIVRDIRIGFGG
                     MAATPTRARTVEDALIGRPWTAATIQAAQSAMDNAFSPLSDMRASAAYRRIVARNLLL
                     KCFLETSEEAAVTRLVPA"
     gene            1628..>2340
                     /locus_tag="VGC82_22745"
     CDS             1628..>2340
                     /locus_tag="VGC82_22745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0426152.1"
                     /translation="MNTASSIGASIPHDSASRHVSGRAVYIDDMAEPPGLLHAFLALS
                     PHAHARIKSIDLSAVVATPGVVAVMTARDVPGVNDVGPAFMGDPIFADGLVEYHGQSI
                     FAVAATSMKLARDAAANAVIEYEILPPILTIDEALAAQSFVLPTQVMQRGDADAALAQ
                     SPLRLSGRIDVGGQEHFYLEGQVAMAIPGEDGDMLVHSSTQHPTEVQHLVARALKLAD
                     HSVVCETRRMGGGFGGKES"
BASE COUNT          421 a          727 c          756 g          436 t
ORIGIN      
        1 ggtgccggtg cgacaacgcc atgacacacg tatgacgaat gcctgctgcg aggtcatata
       61 tatggaacaa acggtcgcat tttgattaaa gttgatgcaa caggtagtct gggagtctgc
      121 gcggcgtttc ttcgttgacc ggttcgcgat aatcggcctt agcttcatcc cgcttaaccg
      181 cttgcatacc ggagggactg aatatcggaa agactggcag cgtgatgcgc gacacgattc
      241 attttcgcct cggacaggaa aatcgagcat tgcgggacat cgacccgaca atgaccgtgc
      301 tggaatacct gcgcgagccg gagcgtctgt acggcaccaa agaagggtgc gccgaaggcg
      361 actgcggcgc ctgttcggtc gtgctcgtgg atcagcaggg ccgctatcag gcggtaaacg
      421 cctgcatcct gttcatgacg gcgctcgatg gcaagcaact gctgacggtt gagcatctgg
      481 cggctgccga cggaaccttg catccggtgc aacaggcgat ggtggaccac catggctccc
      541 aatgtggctt ctgcacgccg ggcttcgtca tgtcgatatt cgccctgcga accgaggcca
      601 aaccggaccg cgccgcggtc aacgaggcgc tggccggcaa tctgtgccgc tgcaccggat
      661 atcgcccgat cgttgatgcg gcgctttcgg tctgtaccgg acatcacaac ccgccagccg
      721 tagcgctacc ggttccacag ggaacactcg ctctgaccaa tggcgacaaa tactcgttct
      781 caccccgctg tggcgatgaa ctggcccgaa ttctactcga ccatccgcag gcggttattg
      841 ttgccggcgg cacggatgtc ggactttggg tgaccaagca gcatcgggca ctgccggtga
      901 ccgtatcgct ggacgcagtg gcggacctgc gggaggtttc cgaaaccgcg gacaccatcc
      961 gcattggcgc ggcggcgacc taccacgacg cgctaccgat gctggagaag catttttccg
     1021 actttggcgc gatgatccgc cgcatcggct cgcggcagat ccgcaaccgg gggaccatcg
     1081 gcggcaacat cgccaacgct tcgccgatcg gggattcggc gccggtcctg ctggcgttgg
     1141 acgcgtcgct ggtcctccgc ctcggcaaca ccagacggac ggtgaagctg gatgacttct
     1201 tcgccggtta tcggcgtacg gtgttggcac cgagcgagtt catcgagcgt atcgatgtgc
     1261 cgatccctcg gcccggagaa ctctttcgct gctacaaggt ctcgaaacgc ttcgaccagg
     1321 acatttcggc ggtgtgcggg gctttccgcc tgacgctcca ggatggcatc gtccgcgaca
     1381 tccgcattgg ctttggcggc atggcggcga cgccgacgcg tgccagaacg gtcgaggacg
     1441 cactgatcgg gcgcccctgg accgcggcta ccatacaggc ggcgcagtcg gcgatggaca
     1501 atgcgttctc cccgctgtcg gatatgcggg cgagtgctgc ctaccgccgg atcgtcgcac
     1561 gcaacctgct gctgaagtgt ttcctggaaa caagcgagga ggcagccgtg acacgtctgg
     1621 tgccggcatg aatacagcct cctccattgg cgcctccatt ccgcacgaca gcgcgtccag
     1681 gcatgtcagc ggccgagcgg tttatattga cgacatggcg gaaccgccgg ggctgctgca
     1741 tgcgtttctg gcgctcagcc cgcacgccca tgctcggatc aagtcgatcg acctgagcgc
     1801 ggtggtcgca acgccgggcg tcgtggcggt gatgacggcc agggatgtcc cgggcgtcaa
     1861 cgacgtcggt cccgccttca tgggcgaccc gatcttcgcc gatgggttgg tcgagtacca
     1921 cgggcagtcg atctttgccg tcgccgccac cagcatgaag ctggctcggg acgctgctgc
     1981 caacgcggtg atcgaatacg aaatcctgcc gcccatcctg acgatcgacg aggcgttggc
     2041 ggcgcaaagc ttcgtgctgc cgacccaggt gatgcagcgc ggcgatgccg atgccgccct
     2101 ggcgcaatcg ccgctgcgcc tgtccggtcg catcgatgtc ggcgggcagg agcactttta
     2161 cctcgaaggc caggttgcca tggcaatccc cggcgaggac ggcgacatgc tggtgcacag
     2221 ttccacccag cacccgaccg aggtgcagca tctggttgcc cgcgcgctga agctggcgga
     2281 tcattcggtg gtgtgcgaaa cgcggcggat gggcggcggc ttcggcggca aggaatcgca
//
LOCUS       DASWQN010001342         3742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_2375089, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001342 DASWQN010000000
VERSION     DASWQN010001342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2375089"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VGC82_22750"
     CDS             <1..258
                     /locus_tag="VGC82_22750"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEY0426153.1"
                     /translation="AEIDHWIRNTVITVNRPLGTCAMGLGSQAVLDPELKVRGIEGLR
                     VVDAAALPDMPSAHINAAVMMLAERASDLIRGRQPLAPLNV"
     gene            complement(286..1746)
                     /gene="gatB"
                     /locus_tag="VGC82_22755"
     CDS             complement(286..1746)
                     /gene="gatB"
                     /locus_tag="VGC82_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY0426154.1"
                     /translation="MSYSLQGSTGPWEVVVGLEVHAQVISRAKLFSGAATVFGAEPNS
                     QVSFVDAAFPGMLPVVNAECVAQAVRTGLGLNARINTVSRFDRKNYFYADLPSGYQIS
                     QFEHPIVGSGEIEVELSDGSSKTIGITRLHLEQDAGKSIHDQHPSKTLIDLNRSGVGL
                     MEIVSEPDIRSPEEAGAYVRKLRSILRYLGTCDGNMEEGSLRADVNVSVRKHGEPYRT
                     RCEVKNVNSIRFVMQAVEAEAARQIEVWESGGTVMQETRLFDSGRGVTRSMRSKEDAH
                     DYRYFPDPDLLPLVLEEDWIASLKASLPELPDAKRARFIQAYGLTPYDASVLVAEKAT
                     AEFYETVAHGRDPKIAANWVTGDFFAALNRFGRSIEDPPVTAAALGGLLDLMADNTIN
                     GRIAKDVFEAMLESGKDAATIVEEKGLRQVTDTGAIDEAVDRIIAANPDKVQEYRSGK
                     DKLFGFFVGQTMKAMAGKGNPGLINETLKRRLAKQG"
     gene            1887..2129
                     /locus_tag="VGC82_22760"
     CDS             1887..2129
                     /locus_tag="VGC82_22760"
                     /inference="COORDINATES: protein motif:HMM:NF019324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEY0426155.1"
                     /translation="MAFHVRDRATDVAVRRLAQVKRKTLTETIREAVEHEYAAVNAVP
                     PLIERLQSIQAQFQAMKQSGGRAADKAFFDELSGQP"
     gene            2129..2524
                     /locus_tag="VGC82_22765"
     CDS             2129..2524
                     /locus_tag="VGC82_22765"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0426156.1"
                     /translation="MFLDASAIIGIIAMEADAASLAERLAGASQVHTSAVAIFEATLG
                     LARIANVPSGDALAVVDRFLTETGGTVVPIDRPTAVAAIEAFDRFGKGHHPAALNMGD
                     CFAYACARQIDAPLLCKGNDFPRTDVNLG"
     gene            complement(2752..>3742)
                     /locus_tag="VGC82_22770"
     CDS             complement(2752..>3742)
                     /locus_tag="VGC82_22770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0426157.1"
                     /translation="SAVAARLAMGATGTDTGGSIRQPASFCGLVGVKPTYGRCSRWGV
                     VAFASSLDHPGPFARTVRDGAILLGSMAGHDPKDSTSAMIAVPDFEAACARGVKGLRI
                     GVPAEYRSDGMPAEIDALWQQGLGWLREAGTEIVDVSLPHTKYGLATYYIVAPAEASS
                     NLARYDGVRFGAREHGTDLRDLYERTRAEGFGAEVRRRILIGTYVLSAGYYDAYYLRA
                     QKIRALILKDFSDAFGQVDALLTPTTPSAAFAQGENMDDPIKMYLNDLFTVPANLAGV
                     PAISVPVGLDANGLPLGLQVIGKSFDEETVFAVSAALEQAACFTALPAIRAGG"
BASE COUNT          683 a         1228 c         1129 g          702 t
ORIGIN      
        1 gccgagatcg accactggat ccgaaatacg gtcatcacgg tcaatcgtcc gcttggcacc
       61 tgcgccatgg gacttggttc gcaggcggtg ctcgacccgg agttgaaggt tcgcggcatc
      121 gaaggtctgc gcgttgtaga tgccgccgcg ctgccggaca tgccgtcggc acatatcaat
      181 gcagccgtga tgatgctggc ggaacgtgcc tctgatctga ttcgtggtcg acagccattg
      241 gctccgctca atgtctgacg gcggcacatg acacgactgt cgggtttacc cctgcttggc
      301 caggcggcgt ttcagggttt cgttgatcag gccgggattg cccttgccgg ccatcgcttt
      361 catcgtctga ccaacgaaga agccgaacag cttgtccttg cctgaacggt attcctgcac
      421 cttgtccggg ttcgcggcga tgatccggtc aacagcttcg tcgatcgcac cggtgtcggt
      481 gacttggcgc aggccctttt cttccacgat ggttgcggcg tccttgccgc tttccaacat
      541 tgcctcgaac acgtccttgg caatgcggcc gttgatggtg ttgtcagcca tcaagtccag
      601 taacccgcca agtgccgcgg cggtcaccgg cggatcctcg atcgaccggc cgaaccggtt
      661 cagcgcggcg aagaaatcgc cggtcaccca gttggcagcg atcttcggat cgcgaccgtg
      721 ggcgaccgtt tcatagaatt cggctgtggc cttttccgcc accagaaccg aggcatcata
      781 tggcgtcagt ccatacgcct ggataaaccg tgcccgcttg gcgtccggca attccggcag
      841 cgacgccttc agcgaggcga tccagtcctc ctccagcacc aacggcagca gatccgggtc
      901 gggaaagtaa cgataatcat gcgcgtcctc cttggaccgc atggatcggg tgacgccgcg
      961 gccgctgtcg aacaagcggg tctcctgcat caccgtgccg ccggactccc aaacctcgat
     1021 ctggcgtgcg gcctccgcct ccaccgcttg catgacgaag cggatggagt tgacgttttt
     1081 cacctcgcac cgggtgcgat acggttcgcc atgcttgcgg acggatacgt tgacgtccgc
     1141 gcgcaaggag ccctcctcca tgttgccgtc gcaggtgccc agatagcgca ggatggagcg
     1201 cagcttgcgg acataggcgc cggcttcctc cgggctgcgg atatcgggtt cggaaacgat
     1261 ctccatcagc ccgacgccgg agcggttcag gtcgatcagc gtcttgctgg gatgctggtc
     1321 gtggatcgac ttgccggcgt cctgttccag atgcagccgg gtgatgccga tcgttttgct
     1381 ggagccgtcg gacagttcca cctcgatctc gccgctgccg acgatcggat gttcgaactg
     1441 gctgatctga tagccggagg gcaggtcggc gtagaagtag ttcttccggt cgaaccggct
     1501 gacggtgttg atgcgcgcgt tcagcccaag gccagtgcgc accgcctgag ccacgcactc
     1561 ggcgttgacg accggcagca tcccgggaaa tgcggcatcc acgaaactga cctgactgtt
     1621 cggttccgca ccaaaaaccg tcgccgcccc actgaacagt ttcgctcggc tgataacctg
     1681 tgcgtggact tccaggccga ccacgacctc ccacggtccg gtgctgcctt gcagtgagta
     1741 gctcatcgct gcgcctttca actgtatccg aatgttgggg atgcgtttag tctggcagac
     1801 ggccggtcgc aacctgtgcg cgatgcgaaa gatttgacca agcgccggcg acccggtata
     1861 cacgacttac tcaaggagtg tatccgatgg cctttcatgt tcgtgatcgc gcgaccgatg
     1921 tggctgtccg caggcttgcg caggtcaagc gcaaaacgct gaccgagacg atccgtgaag
     1981 cggtggaaca cgaatacgcc gcggtcaacg cggttccgcc cctcatcgag cggctgcaat
     2041 cgatccaggc gcagttccag gcgatgaagc aaagcggcgg acgtgcggcg gacaaggctt
     2101 ttttcgatga gttgtccggg cagccgtgat gttcctcgat gcctcggcga tcattggcat
     2161 catcgcaatg gaggcggatg ctgccagcct ggccgaacgg ttagcgggag catcccaggt
     2221 gcatacatcg gctgtggcga ttttcgaagc cacgctgggg ctggcgcgca tcgcgaacgt
     2281 gccttccggt gacgctttgg ccgttgtcga tcgtttcctg actgagaccg gcgggacggt
     2341 tgtgccgatc gaccgcccaa ccgcagttgc ggcgatcgag gcgttcgacc gtttcggcaa
     2401 gggccatcac ccggccgccc tgaacatggg tgactgcttt gcctacgcct gcgcacggca
     2461 gatcgacgcg ccgttgctgt gcaaaggaaa cgatttcccg cgcaccgatg tcaaccttgg
     2521 ctagagcatg atcacgctga gcggaacgtt cagagccgga tcacacgatc caacaagaag
     2581 gtagagcatt ctcagcctat tcggtcaggc cgagaatgct ccagcgattg tcctgcccgc
     2641 gaagttcgcc gcatcacgcg gcggacgcag gggatcagca tgccactgaa aacaaagaga
     2701 gtggcgcgag actgaggtcc gccggatatg gcggacttca gattcgggac tctagccccc
     2761 tgccctgatc gccggcagcg cggtgaagca agctgcctgc tccagcgccg cggataccgc
     2821 aaacacggtt tcctcatcga acgactttcc gatcacctga agccccagcg gcagcccgtt
     2881 ggcgtcgagg ccgaccggta cagagatcgc gggcacaccg gccaggttgg ccggcacggt
     2941 gaacaggtcg ttcaaataca tcttgatggg atcgtccatg ttctcaccct gggcgaacgc
     3001 agccgacggc gtggtcggcg tcagcagcgc gtccacctga ccgaacgcgt cgctgaaatc
     3061 cttcaggatc agcgcccgga ttttctgtgc ccgcaggtaa taagcgtcat agtagccggc
     3121 agacagtaca taggttccaa tcaggatccg ccgccgcacc tccgccccga acccctcggc
     3181 gcgggtccgc tcgtacaggt cccgcaagtc cgtcccatgc tctcgagcgc cgaaccgcac
     3241 gccgtcgtac cgggccaaat tggaagacgc ctctgccggc gcaacgatat aatacgtcgc
     3301 cagcccatat ttcgtgtgcg gcagtgacac gtcaacaatc tcagtcccgg cctcgcgcag
     3361 ccagcccagg ccctgctgcc acagcgcgtc gatctccgcc ggcatgccgt ccgaccgata
     3421 ctccgccggg acaccgatcc gcagcccctt cacgccgcgc gcgcaggccg cctcaaaatc
     3481 cggcaccgcg atcatcgcgc tggttgagtc ctttggatca tgccccgcca tcgaccccag
     3541 caggatcgcc ccatcgcgca ccgtccgcgc gaacggcccg gggtgatcca gcgacgatgc
     3601 gaacgccacc accccccagc gggaacaccg cccataggtc ggctttaccc ccaccagccc
     3661 gcagaacgac gccggctggc ggatcgaccc gccggtatca gtcccggtcg cccccatcgc
     3721 cagccgcgcc gcgaccgcgg ac
//
LOCUS       DASWQN010001343         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhodopila sp. isolate SMAG_U3496 k141_791014, whole
            genome shotgun sequence.
ACCESSION   DASWQN010001343 DASWQN010000000
VERSION     DASWQN010001343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959312
            Sequence Read Archive: SRR10998589
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodopila sp. (soil metagenome)
  ORGANISM  Rhodopila sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae; Rhodopila.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:36:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,556
            CDSs (total)                      :: 4,525
            Genes (coding)                    :: 4,472
            CDSs (with protein)               :: 4,472
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 24 of 53
            Pseudo Genes (incomplete)         :: 32 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 11 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Rhodopila sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791014"
                     /isolate="SMAG_U3496"
                     /isolation_source="soil"
                     /db_xref="taxon:2480087"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="38.7966 N 120.3833 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..388)
                     /locus_tag="VGC82_22775"
     CDS             complement(83..388)
                     /locus_tag="VGC82_22775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HEY0426158.1"
                     /translation="MTARIFKPAKNAMQSGRAKTREWQLDYEPEQPRAIEPLMGWTSS
                     GDMKQQLTLQFDTREDAVAYCERNGIAYQVIEPKDSKQRQVAYSDNFAFRRGEPWTH"
     gene            636..1781
                     /locus_tag="VGC82_22780"
     CDS             636..1781
                     /locus_tag="VGC82_22780"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEY0426159.1"
                     /translation="MKINAILPEKGRDLRLDLFRGVANWAIYLDHIPDNVVNWITTRN
                     YGFSDAADLFVFISGYTASFVYARMMLDRGFLVGATRLTKRVWQLYVAHIILFVIYIA
                     SISYLALRFGDSELVNEFNVVGLVDNATETLRQGLFLKFKPVNLDVLPLYIVLMGFFP
                     PVLWMMLRQPDLTMAASIVLWLAARYFGWNFAAYPVGTWYFNPYAWQVLFVFGSWCAL
                     GGARKSMAIINAPVTLYFCLAYLVFALVMTMAGRFPDFAALLPHWLDSAFNPNDKTNL
                     APYRFLHFVVIVVMVIRFVPKGWPGLEWKGFDPLIVCGQQSLAVFCVGVFLSFVGHFT
                     LMMSSGSLLAQILVSVAGIAIMTTVAYYISWSKRQDKPLKPAAPKAA"
BASE COUNT          305 a          574 c          536 g          431 t
ORIGIN      
        1 tccccgcaac cggtccccga accgacccct ggcgctaaag cgccttagag ggctccagga
       61 cccttcttgg tgggcccgaa gcttagtgcg tccagggctc gccgcgccgg aacgcaaaat
      121 tgtcggaata ggcgacctgc cgctgcttgg aatccttcgg ctcgatcacc tgataggcaa
      181 tgccattgcg ctcgcaatag gccaccgcgt cttccctggt gtcgaattgc agcgtgagct
      241 gctgcttcat gtcgccggag gaggtccagc ccatcaaggg ttcgatcgcc cggggctgct
      301 cgggctcgta atcgagctgc cattcccggg tcttggccct gcccgattgc atcgcgtttt
      361 tggcgggctt aaaaatgcgt gcggtcatgg atcgtaggac ccccagagat atgcgactgc
      421 gacgttttga cgggacattt ggcggcaacg gctgggatag tatagagaca cctttcagga
      481 atgtgatcgg tgattccaaa accctaccat ttccggaccg gtatagtcat tatgctgcac
      541 tgtgacaatc ttcggccgcc cggcggtcaa ggtatggtac gagccctggt cttgcggcac
      601 gatcctcccg aaagtatcac ctcgaagcgg cacccatgaa aatcaacgct atcctgcccg
      661 aaaaaggacg cgacctccgg ctcgacctgt ttcgcggggt cgcaaactgg gcgatctatc
      721 tggatcacat tccagacaat gtcgtgaact ggatcaccac ccgcaattac ggcttcagtg
      781 acgccgccga cctgttcgtt ttcatctccg gatataccgc ctccttcgtc tatgcccgga
      841 tgatgctgga ccgcggcttc ctcgtcggcg ccacgcggct gaccaagcgc gtctggcagc
      901 tctatgtcgc ccatatcatc ctgttcgtga tctatattgc ctcgatcagc tatctggcgc
      961 tgcgctttgg cgattctgag ctggtcaacg agttcaacgt cgtcggcctg gtcgacaacg
     1021 ccaccgagac gttgcggcaa ggcctgttcc tcaagttcaa gccggtcaat ctcgacgtgc
     1081 tgccgctcta catcgtgctg atgggctttt ttccaccggt gctgtggatg atgctgcgcc
     1141 agcccgacct gaccatggcc gcctccatcg tgttgtggtt ggcggcgcgc tatttcggct
     1201 ggaattttgc cgcctatccg gtcggcacct ggtatttcaa tccgtatgcc tggcaggtgc
     1261 tgtttgtgtt cgggtcctgg tgcgcgctgg gcggcgcccg caagtcgatg gccatcatca
     1321 atgcgccggt gacgctgtat ttctgtctcg cctatctcgt gttcgcgctg gtcatgacca
     1381 tggccggccg ttttccggat ttcgcggcgc tgctgccgca ttggctcgat tcggccttca
     1441 atccgaacga caagaccaac ctcgctccct accggttcct gcatttcgtg gtgatcgtgg
     1501 tcatggtgat ccgcttcgtc cccaagggct ggccgggact ggaatggaaa ggattcgatc
     1561 cgctgatcgt ctgcggccag cagtcgctgg ccgtgttctg tgtcggcgtg ttcctgtcct
     1621 tcgttgggca cttcaccctg atgatgagtt ccggctcgct gcttgcgcag atcctggtga
     1681 gcgtggccgg catcgccatc atgacaacag tcgcctacta catttcctgg tcgaagcggc
     1741 aggacaagcc gctcaagccg gcggcgccga aagccgcctg agcggcgccc ggctgtgctt
     1801 gggcagccgc cttcaaatcg gctgcgcggt cgacgccacc agtgtc
//
